LOCUS       DAPHBJ010000001        85031 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.001, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000001 DAPHBJ010000000
VERSION     DAPHBJ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 85031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.001"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..159
                     /locus_tag="RHZ76_000001"
     CDS             <1..159
                     /locus_tag="RHZ76_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HDV2810709.1"
                     /translation="FIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKII
                     GGCGPTAQ"
     gene            403..1791
                     /gene="frlA"
                     /locus_tag="RHZ76_000002"
     CDS             403..1791
                     /gene="frlA"
                     /locus_tag="RHZ76_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HDV2810710.1"
                     /translation="MTANSPLQRIGQEKGIAMGSQELQRKLGFWAVLAIAVGTTVGSG
                     IFVSVGEVAKAAGTPWLTVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGS
                     RPLAFLSGWASFWANDAPSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHL
                     RSVEGGAAFQTLITIAKIIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGI
                     SATSWSYTGMASICYMTGEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKL
                     ANSETPISDALTWIPALGSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFF
                     KCFGHVHPKYNTPDVSIILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWC
                     RKRDDYKPLWRTPAFGLMTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAF
                     WAKRSRQLNALS"
     gene            1812..2834
                     /gene="frlB"
                     /locus_tag="RHZ76_000003"
     CDS             1812..2834
                     /gene="frlB"
                     /locus_tag="RHZ76_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HDV2810711.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            2884..3714
                     /gene="frlC"
                     /locus_tag="RHZ76_000004"
     CDS             2884..3714
                     /gene="frlC"
                     /locus_tag="RHZ76_000004"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HDV2810712.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIERGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            3711..4496
                     /gene="frlD"
                     /locus_tag="RHZ76_000005"
     CDS             3711..4496
                     /gene="frlD"
                     /locus_tag="RHZ76_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HDV2810713.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDETLRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAIAQGTACAAKTIQYHGAW"
     gene            4596..5327
                     /gene="phoB"
                     /locus_tag="RHZ76_000006"
     CDS             4596..5327
                     /gene="phoB"
                     /locus_tag="RHZ76_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HDV2810714.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(5479..6564)
                     /locus_tag="RHZ76_000007"
     CDS             complement(5479..6564)
                     /locus_tag="RHZ76_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDV2810715.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(6576..7880)
                     /locus_tag="RHZ76_000008"
     CDS             complement(6576..7880)
                     /locus_tag="RHZ76_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HDV2810716.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENGIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(7892..8245)
                     /locus_tag="RHZ76_000009"
     CDS             complement(7892..8245)
                     /locus_tag="RHZ76_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HDV2810717.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(8256..9134)
                     /locus_tag="RHZ76_000010"
     CDS             complement(8256..9134)
                     /locus_tag="RHZ76_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HDV2810718.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(9131..10357)
                     /locus_tag="RHZ76_000011"
     CDS             complement(9131..10357)
                     /locus_tag="RHZ76_000011"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDV2810719.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFCDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDKKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPAAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(10357..11520)
                     /locus_tag="RHZ76_000012"
     CDS             complement(10357..11520)
                     /locus_tag="RHZ76_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HDV2810720.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRIQSVLLKVYSDDDFLY
                     PGQESGFALKVLPEIVAEIQNLPGLHLAGLTHFPCLLWDEAVGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPEIVGRYDSLGNSLGA"
     gene            complement(11604..11966)
                     /locus_tag="RHZ76_000013"
     CDS             complement(11604..11966)
                     /locus_tag="RHZ76_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDV2810721.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(11983..12888)
                     /locus_tag="RHZ76_000014"
     CDS             complement(11983..12888)
                     /locus_tag="RHZ76_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2810722.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELANECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLIYRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(13178..14182)
                     /gene="trpS"
                     /locus_tag="RHZ76_000015"
     CDS             complement(13178..14182)
                     /gene="trpS"
                     /locus_tag="RHZ76_000015"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDV2810723.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(14175..14933)
                     /gene="gph"
                     /locus_tag="RHZ76_000016"
     CDS             complement(14175..14933)
                     /gene="gph"
                     /locus_tag="RHZ76_000016"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HDV2810724.1"
                     /translation="MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(14926..15603)
                     /gene="rpe"
                     /locus_tag="RHZ76_000017"
     CDS             complement(14926..15603)
                     /gene="rpe"
                     /locus_tag="RHZ76_000017"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDV2810725.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(15621..16457)
                     /gene="dam"
                     /locus_tag="RHZ76_000018"
     CDS             complement(15621..16457)
                     /gene="dam"
                     /locus_tag="RHZ76_000018"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDV2810726.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(16564..17850)
                     /gene="damX"
                     /locus_tag="RHZ76_000019"
     CDS             complement(16564..17850)
                     /gene="damX"
                     /locus_tag="RHZ76_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HDV2810727.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRETAKTQTAERPATTRPARQQAVIEPKKSQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(17942..19030)
                     /gene="aroB"
                     /locus_tag="RHZ76_000020"
     CDS             complement(17942..19030)
                     /gene="aroB"
                     /locus_tag="RHZ76_000020"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDV2810728.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(19087..19608)
                     /gene="aroK"
                     /locus_tag="RHZ76_000021"
     CDS             complement(19087..19608)
                     /gene="aroK"
                     /locus_tag="RHZ76_000021"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HDV2810729.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(20009..21247)
                     /gene="hofQ"
                     /locus_tag="RHZ76_000022"
     CDS             complement(20009..21247)
                     /gene="hofQ"
                     /locus_tag="RHZ76_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HDV2810730.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGNDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPLFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(21159..21563)
                     /gene="hofP"
                     /locus_tag="RHZ76_000023"
     CDS             complement(21159..21563)
                     /gene="hofP"
                     /locus_tag="RHZ76_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HDV2810731.1"
                     /translation="MRVKRWLLAGVALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGEHIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTSGKAAKSDADGG"
     gene            complement(21553..21993)
                     /gene="hofO"
                     /locus_tag="RHZ76_000024"
     CDS             complement(21553..21993)
                     /gene="hofO"
                     /locus_tag="RHZ76_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HDV2810732.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAVWLLMLVTLIFLSSTHQEESDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(21977..22516)
                     /gene="hofN"
                     /locus_tag="RHZ76_000025"
     CDS             complement(21977..22516)
                     /gene="hofN"
                     /locus_tag="RHZ76_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HDV2810733.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQGEQQLAHSLQITKPRLLEQQQLREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(22516..23295)
                     /gene="hofM"
                     /locus_tag="RHZ76_000026"
     CDS             complement(22516..23295)
                     /gene="hofM"
                     /locus_tag="RHZ76_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HDV2810734.1"
                     /translation="MAFKIWQIGLHLQQQEALAVAIVRDAKECFLQRWWRLPLAHDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQMAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQQFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            23415..25967
                     /gene="mrcA"
                     /locus_tag="RHZ76_000027"
     CDS             23415..25967
                     /gene="mrcA"
                     /locus_tag="RHZ76_000027"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HDV2810735.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(26135..26695)
                     /gene="nudE"
                     /locus_tag="RHZ76_000028"
     CDS             complement(26135..26695)
                     /gene="nudE"
                     /locus_tag="RHZ76_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HDV2810736.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            27015..29150
                     /gene="igaA"
                     /locus_tag="RHZ76_000029"
     CDS             27015..29150
                     /gene="igaA"
                     /locus_tag="RHZ76_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HDV2810737.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANTGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            29215..29883
                     /gene="yrfG"
                     /locus_tag="RHZ76_000030"
     CDS             29215..29883
                     /gene="yrfG"
                     /locus_tag="RHZ76_000030"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HDV2810738.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLSVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            29894..30295
                     /gene="hslR"
                     /locus_tag="RHZ76_000031"
     CDS             29894..30295
                     /gene="hslR"
                     /locus_tag="RHZ76_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HDV2810739.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            30320..31198
                     /gene="hslO"
                     /locus_tag="RHZ76_000032"
     CDS             30320..31198
                     /gene="hslO"
                     /locus_tag="RHZ76_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDV2810740.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(31261..32985)
                     /locus_tag="RHZ76_000033"
     CDS             complement(31261..32985)
                     /locus_tag="RHZ76_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDV2810741.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLSGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFNTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGVLAVLVLLVWSLGY
                     FVSIVWRKGQNPVVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TSDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQQQVSEKV
                     LADNVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLNVNLNE"
     gene            33364..34986
                     /gene="pckA"
                     /locus_tag="RHZ76_000034"
     CDS             33364..34986
                     /gene="pckA"
                     /locus_tag="RHZ76_000034"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDV2810742.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(35062..36414)
                     /gene="envZ"
                     /locus_tag="RHZ76_000035"
     CDS             complement(35062..36414)
                     /gene="envZ"
                     /locus_tag="RHZ76_000035"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HDV2810743.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(36411..37130)
                     /gene="ompR"
                     /locus_tag="RHZ76_000036"
     CDS             complement(36411..37130)
                     /gene="ompR"
                     /locus_tag="RHZ76_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HDV2810744.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            37358..37834
                     /gene="greB"
                     /locus_tag="RHZ76_000037"
     CDS             37358..37834
                     /gene="greB"
                     /locus_tag="RHZ76_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HDV2810745.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            37931..40252
                     /locus_tag="RHZ76_000038"
     CDS             37931..40252
                     /locus_tag="RHZ76_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDV2810746.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            40690..40917
                     /gene="feoA"
                     /locus_tag="RHZ76_000039"
     CDS             40690..40917
                     /gene="feoA"
                     /locus_tag="RHZ76_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HDV2810747.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            40934..43255
                     /gene="feoB"
                     /locus_tag="RHZ76_000040"
     CDS             40934..43255
                     /gene="feoB"
                     /locus_tag="RHZ76_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HDV2810748.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            43255..43491
                     /gene="feoC"
                     /locus_tag="RHZ76_000041"
     CDS             43255..43491
                     /gene="feoC"
                     /locus_tag="RHZ76_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HDV2810749.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            43694..44572
                     /gene="rpnA"
                     /locus_tag="RHZ76_000042"
     CDS             43694..44572
                     /gene="rpnA"
                     /locus_tag="RHZ76_000042"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HDV2810750.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYAYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(44601..45371)
                     /gene="bioH"
                     /locus_tag="RHZ76_000043"
     CDS             complement(44601..45371)
                     /gene="bioH"
                     /locus_tag="RHZ76_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HDV2810751.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            45409..46092
                     /gene="gntX"
                     /locus_tag="RHZ76_000044"
                     /pseudo
     CDS             45409..46092
                     /gene="gntX"
                     /locus_tag="RHZ76_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
     gene            46151..46726
                     /gene="nfuA"
                     /locus_tag="RHZ76_000045"
     CDS             46151..46726
                     /gene="nfuA"
                     /locus_tag="RHZ76_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HDV2810752.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            47087..48403
                     /gene="gntT"
                     /locus_tag="RHZ76_000046"
     CDS             47087..48403
                     /gene="gntT"
                     /locus_tag="RHZ76_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDV2810753.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(48448..50532)
                     /gene="malQ"
                     /locus_tag="RHZ76_000047"
     CDS             complement(48448..50532)
                     /gene="malQ"
                     /locus_tag="RHZ76_000047"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDV2810754.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(50542..52935)
                     /gene="malP"
                     /locus_tag="RHZ76_000048"
     CDS             complement(50542..52935)
                     /gene="malP"
                     /locus_tag="RHZ76_000048"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HDV2810755.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            53547..56252
                     /gene="malT"
                     /locus_tag="RHZ76_000049"
     CDS             53547..56252
                     /gene="malT"
                     /locus_tag="RHZ76_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HDV2810756.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMTQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREDTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQDAVQHAQQLLKMMGYGV"
     gene            complement(56295..57311)
                     /gene="rtcA"
                     /locus_tag="RHZ76_000050"
     CDS             complement(56295..57311)
                     /gene="rtcA"
                     /locus_tag="RHZ76_000050"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HDV2810757.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLLFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKMGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     TAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFSLIETDGVTR
                     VSIE"
     gene            complement(57315..58541)
                     /gene="rtcB"
                     /locus_tag="RHZ76_000051"
     CDS             complement(57315..58541)
                     /gene="rtcB"
                     /locus_tag="RHZ76_000051"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HDV2810758.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            58730..60328
                     /gene="rtcR"
                     /locus_tag="RHZ76_000052"
     CDS             58730..60328
                     /gene="rtcR"
                     /locus_tag="RHZ76_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HDV2810759.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWLPTLCLCQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTQLLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEALQDQHSSS"
     gene            complement(60310..61068)
                     /locus_tag="RHZ76_000053"
     CDS             complement(60310..61068)
                     /locus_tag="RHZ76_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HDV2810760.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAQPPVSVMQVLTDHHIQLELC"
     gene            complement(61085..61915)
                     /gene="glpG"
                     /locus_tag="RHZ76_000054"
     CDS             complement(61085..61915)
                     /gene="glpG"
                     /locus_tag="RHZ76_000054"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HDV2810761.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(61960..62286)
                     /gene="glpE"
                     /locus_tag="RHZ76_000055"
     CDS             complement(61960..62286)
                     /gene="glpE"
                     /locus_tag="RHZ76_000055"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HDV2810762.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            62476..63981
                     /gene="glpD"
                     /locus_tag="RHZ76_000056"
     CDS             62476..63981
                     /gene="glpD"
                     /locus_tag="RHZ76_000056"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HDV2810763.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLNVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(64197..64799)
                     /locus_tag="RHZ76_000057"
     CDS             complement(64197..64799)
                     /locus_tag="RHZ76_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2810764.1"
                     /translation="MMNIIRLYIYPLFLGFVIVPLLVWPMVVALAACIITLSFLGEML
                     FSIPLMIRRISLLQLQLWLSAEYALFFCVMCCVGWQFTRRTQSELKSRLHCWLVFAPV
                     YLWLILWNIIFYIAPAKIALLESIRNFFLLVVWLPLNFSPFWPQPWTDFIGPISAQLG
                     FALGYYCQWCSKNRCQIRKLSEWGTCLSFVTLGMMAWALR"
     gene            complement(64799..65791)
                     /locus_tag="RHZ76_000058"
     CDS             complement(64799..65791)
                     /locus_tag="RHZ76_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDV2810765.1"
                     /translation="MNKFTGVLLLGTALLAGCVDREGYYNSVREEESHGLTSLRGQPA
                     LRYSDDWSRWPRVYGATALYPLYASAYYKLVPEPKDKDRTSLAWQAYGLQQTRTAEAY
                     DSLIKGSATVIFVAQPSEGQKKRAEEAGVKLKYTAFAREAFVFIVDINNPVNSLSEHQ
                     VKDIFSGKTSRWNKVGGSDEHIKVWQRPEDSGSQTIMKGLVMQDTPMLPAKKSTVIDL
                     MGGLITEVADYQNTPSSIGYTFHYYVTRMNDNMLKMRKHIKLLAINGVAPTEENIRNG
                     TYPYIVDAYMVTRENPTPETQKFVDWFISQQGQQLVEDVGYVPLYEASPESSGQ"
     gene            complement(65817..67325)
                     /locus_tag="RHZ76_000059"
     CDS             complement(65817..67325)
                     /locus_tag="RHZ76_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDV2810766.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NIFITPTIYLLLNVFMLTLGAIIIFFSGRVWAGDSAPENREIAVWRQCFFLLPALLTL
                     VGWIITLHLADYQFRQMGAGWLANLMLPWLGVFLVSLVGGEYWWMVIIPVGAHISFSL
                     GYAWPTRYPLSGTSGLRCRNLLLFLLLLLGIVAGYQAHLYKQQNPGVGVRENIDIWAW
                     RPDKLNNRLTPLRGKPQIQFRQNWPRIDGATAAYPIYASAFYALSVISEDFHVWDYLE
                     NSRTPEAYNKIVKGDADIIFVAQPSGGQKKRAKESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRAVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETKVASVMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGITPTAENI
                     RNGKYAYIVDAFMVTRENTTSETQKLVEWFLTPQGQSLVEDVGYVPLYPTME"
     gene            complement(67450..69897)
                     /gene="glgP"
                     /locus_tag="RHZ76_000060"
     CDS             complement(67450..69897)
                     /gene="glgP"
                     /locus_tag="RHZ76_000060"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HDV2810767.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(69916..71349)
                     /gene="glgA"
                     /locus_tag="RHZ76_000061"
     CDS             complement(69916..71349)
                     /gene="glgA"
                     /locus_tag="RHZ76_000061"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDV2810768.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(71349..72644)
                     /gene="glgC"
                     /locus_tag="RHZ76_000062"
     CDS             complement(71349..72644)
                     /gene="glgC"
                     /locus_tag="RHZ76_000062"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDV2810769.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(72662..74635)
                     /gene="glgX"
                     /locus_tag="RHZ76_000063"
     CDS             complement(72662..74635)
                     /gene="glgX"
                     /locus_tag="RHZ76_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HDV2810770.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSVHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(74632..76818)
                     /gene="glgB"
                     /locus_tag="RHZ76_000064"
     CDS             complement(74632..76818)
                     /gene="glgB"
                     /locus_tag="RHZ76_000064"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HDV2810771.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGARFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(77091..78194)
                     /gene="asd"
                     /locus_tag="RHZ76_000065"
     CDS             complement(77091..78194)
                     /gene="asd"
                     /locus_tag="RHZ76_000065"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDV2810772.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            78386..78979
                     /gene="yhgN"
                     /locus_tag="RHZ76_000066"
     CDS             78386..78979
                     /gene="yhgN"
                     /locus_tag="RHZ76_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HDV2810773.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(79036..80376)
                     /gene="gntU"
                     /locus_tag="RHZ76_000067"
     CDS             complement(79036..80376)
                     /gene="gntU"
                     /locus_tag="RHZ76_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDV2810774.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(80380..80907)
                     /gene="gntK"
                     /locus_tag="RHZ76_000068"
     CDS             complement(80380..80907)
                     /gene="gntK"
                     /locus_tag="RHZ76_000068"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDV2810775.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(81046..82041)
                     /gene="gntR"
                     /locus_tag="RHZ76_000069"
     CDS             complement(81046..82041)
                     /gene="gntR"
                     /locus_tag="RHZ76_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HDV2810776.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLLLTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(82265..82960)
                     /gene="yhhW"
                     /locus_tag="RHZ76_000070"
     CDS             complement(82265..82960)
                     /gene="yhhW"
                     /locus_tag="RHZ76_000070"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HDV2810777.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(83083..84120)
                     /locus_tag="RHZ76_000071"
     CDS             complement(83083..84120)
                     /locus_tag="RHZ76_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDV2810778.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFSADDSVGV
                     LEYVNDEGVTVREEIKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            84453..84941
                     /gene="yhhY"
                     /locus_tag="RHZ76_000072"
     CDS             84453..84941
                     /gene="yhhY"
                     /locus_tag="RHZ76_000072"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV2810779.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSDYMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSHWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT        20443 a        23255 c        21383 g        19950 t
ORIGIN      
        1 tttattaaag ttgcactggt aggtgcagta ctggctacgt taactgcatg tactggtcat
       61 attgaaaacc gtgataagaa ctgctcttac gactacctgc tgcacccggc aatttctatt
      121 tctaaaatca ttggcggttg cggtcctact gcacagtaag tctctgacag aaatcggcta
      181 acaccggttt aattgcccga cgccctctgg attttccgag ggcgtatttg tttctatgtc
      241 cttcttgccc ccgcccgtaa aaatattttc actttaaatt caatttgata actacatcca
      301 ttagccacag cgcgatcctg tgcgaaattt tgtgatcttc ctccacatta cataacatca
      361 tatgttgtta tattcatcat gcattgtcat gttacctttt aaatgactgc aaactctccc
      421 ctacaacgta ttggacaaga aaaaggtatc gctatgggaa gccaggaact ccaacgcaag
      481 ctcggatttt gggccgttct tgcaatcgcc gtcgggacaa ccgtcggctc cggtattttt
      541 gtatctgtgg gtgaagtggc aaaagcagcg ggcacgccgt ggcttacggt gctcgcgttt
      601 gtcattggcg ggttaattgt gatcccgcaa atgtgcgtct atgcggaact atccaccgct
      661 tatccggaaa atggcgcaga ttatgtttat ctgaaaaatg ccggaagccg accgctggct
      721 ttcctctccg gctgggccag cttctgggcc aacgatgcgc cgtcattgtc gattatggcg
      781 ctggcgattg tcagcaatct tggcttttta acgcctatcg atccgttgct cggtaaattt
      841 atcgccgccg gattaattat cgcctttatg ttgctacacc tgcgctccgt tgaaggcggc
      901 gcagcgtttc agacgctaat taccatcgcc aaaattatcc cgttcactat cgtcattggc
      961 cttgggatct tctggtttaa agcggagaat tttgccgccc ctaccaccac tgcgattggc
     1021 gcaacgggca gctttatggc gctgctggcg gggatctctg ccaccagttg gtcgtatacc
     1081 ggcatggcct ctatctgtta tatgaccggc gaaattaaaa accccggaaa aaccatgcca
     1141 cgagcgctga ttggttcctg tctgctggtt ctggtgctct acaccctgct ggcgctggtg
     1201 atttccggcc tgatgccctt cgacaaactc gccaattctg aaacgccgat ttccgacgcc
     1261 ctgacctgga tccccgcact cggcagcacc gctgggatct ttgttgccat cacggcgatg
     1321 atcgtcattc ttggttcgct ttccagctgc gtgatgtacc agccgcggct ggaatacgcg
     1381 atggcgaaag acaacctgtt ctttaaatgc ttcggccatg tgcatccgaa atacaacacg
     1441 ccggatgtct ccatcatcct gcaaggggcg ctggggatct tcttcatctt cgtttccgat
     1501 ctcaccagcc tgctgggtta tttcaccctg gtgatgtgtt tcaaaaatac cctcaccttc
     1561 ggctccatca tctggtgtcg taaacgcgac gattacaaac cgctgtggcg tactccggct
     1621 ttcgggctga tgaccaccct cgccattgcg tcaagcctca ttctggtcgc ctcaaccttt
     1681 gtctgggcac cgattcccgg ccttatctgc gccgtcatcg ttattgctac tggtctgcct
     1741 gcttacgcct tctgggcgaa gcgtagccgc cagctcaacg ctttgtcgta atgttatctg
     1801 gagaaaataa aatgttggat attgataaaa gcaccgtgga ctttctggtc accgaaaata
     1861 tggttcagga agtggaaaaa gttctcagcc atgacgttcc gctggtgcac gccatcgtgg
     1921 aagagatggt gaagcgcgac attgatcgta tttatttcgt tgcctgcggc tcgccactca
     1981 acgcggcgca aacggcgaaa catctggcgg atcgcttttc cgatcttcag gtctacgcca
     2041 tttccggctg ggagttctgc gataacaccc cgtatcgcct cgacgatcgt tgcgcggtga
     2101 ttggcgtttc tgactacggt aaaacggaag aggtaatcaa agcgctggag ctgggccggg
     2161 cctgcggcgc actcactgcg gcgttcacca aacgcgcgga cagcccgatt acctcggcgg
     2221 cggaatttag cattgattat caggccgact gtatctggga aattcacctg ctgctctgct
     2281 acagcgtggt gctggagatg atcacccgcc tcgcgccgaa cgcggaaatc ggcaagatca
     2341 aaaacgatct caagcagttg ccgaatgccc tcggtcatct ggtacgcacc tgggaagaaa
     2401 aaggccgcca gcttggtgaa ctggccagcc agtggccgat gatttatacc gttgctgcgg
     2461 gtccgctgcg tccgctgggt tacaaagaag gcattgtaac gctgatggaa tttacctgga
     2521 cgcacggctg cgtgattgag agcggagagt tccgccatgg cccgctggag attgtcgaac
     2581 cgggcgttcc gttcctgttc ctgctcggca atgatgaaag tcgccacacc accgaacgcg
     2641 ccattaactt tgttaaacag cgtactgaca acgtgatcgt catcgattac gccgaaattt
     2701 cgcaagggct gcacccgtgg ctggcaccgt tcctgatgtt cgtgccaatg gagtggctct
     2761 gctactacct gtctatttac aaagatcaca acccggatga acgccgctat tacggtggtc
     2821 tggtggaata ttaatccctc tcccggcccg gcaacgggcc ggatttacgc aaggagttac
     2881 ccgatgaaaa caggtatgtt tacctgcggc caccagcggc tgcctattga acacgcattt
     2941 cgtgatgcaa gcgagctggg ttacgacggc atcgaaattt ggggcggtcg cccgcacgcg
     3001 ttcgcgccgg acttaaaagc gggcggcatc aaacaaatca aggcgctggc gcagacgtat
     3061 cagatgccga ttatcggcta tacgccagaa accaacggct atccgtataa catgatgctg
     3121 ggcgatgaac atatgcgtcg cgaaagcctc gacatgatca agctggcgat ggatatggca
     3181 aaagagatga acgcgggtta tacgctgatt tccgcggccc atgcgggcta tctcacgcca
     3241 cctaatgtta tctggggacg gctggcagag aacctaagcg aactgtgtga gtacgcggaa
     3301 aacatcggca tggatctaat cctcgaaccg ttaacgccgt atgaatcgaa cgtcgtgtgt
     3361 aatgctaatg atgtgcttca tgcgctggcg ctggtgcctt cgccgcgcct gttcagcatg
     3421 gtcgacattt gcgcgccgta tgtccaggcg gaaccggtga tgagttattt cgacaaactg
     3481 ggcgataaat tacgtcatct gcatattgtc gacagcgacg gggccagcga cacgcattac
     3541 attcctggag aaggcaaaat gccgctgcgg gaactgatgc gcgatattat tgagcggggc
     3601 tatgagggtt actgtacggt ggagctggtg acgatgtata tgaacgagcc cagactctat
     3661 gcccgccagg cgctggaacg ctttcgcgcg ctgctgccgg aggatgagag atgaaaaccc
     3721 tggcgacaat cggcgataac tgcgtcgata tctacccgca actgaataaa gcgttttctg
     3781 gcggtaatgc ggtcaatgtg gcggtgtact gcactcgcta cggcatacag ccgggatgca
     3841 ttacctgggt gggtgacgat gactacggca caaagctgaa gcaggatctc gcccgcatgg
     3901 gcgtcgatat cagccatgtc catacgaaac acggcgttac cgcacaaact caggtggaac
     3961 tgcacgacaa tgatcgcgtt tttggcgact acaccgaagg cgtgatggcc gactttgccc
     4021 tgagtgaaga ggattacgcc tggctggcgc agtatgacat tgtgcacgcc gcaatctggg
     4081 gacatgcgga agacgcattc ccacagctgc acgctgcggg caaacttacc gctttcgact
     4141 tctccgacaa gtgggacagc ccgctctggc agacactggt gccgcatctc gattttgcct
     4201 ttgcctccgc accgcaagaa gacgaaacgc tgcgtctgaa gatgaaagcg attgttgccc
     4261 gtggcgcagg aacagtgatt gtcacgctgg gtgaaaacgg cagcattgcc tgggatggcg
     4321 cgcagttctg gcgtcaggct cctgaaccgg tgacggttat cgacaccatg ggtgccggag
     4381 attcgttcat tgccggattc ctttgcggct ggtctgcggg gatgacatta ccgcaggcga
     4441 tagcgcaggg aacggcgtgc gcggcgaaaa ccattcagta ccacggtgcc tggtaggtat
     4501 aacgttggcg tgagcatctt cacgccaacg tgctgttact tgccggaaaa cgaccctata
     4561 atccgagtaa ttcattcttt atttcagggt cgattatgtc agctacggac cgctactctc
     4621 atcaactcct ctacgctacc gtccgccagc gactgctgga tgatatcgcg cagggggttt
     4681 accaggccgg gcaacagatc cctaccgaaa acgagctttg tacacaatat aacgtcagcc
     4741 gcattaccat tcgcaaagcc atcagcgact tagtggcaga cggcgtactg atccgctggc
     4801 agggaaaagg cacctttgta caaagccaga aagttgaaaa cgccctgctt actgtcagtg
     4861 gttttaccga ttttggcgtc tcacaaggca aggcgacgaa agagaaagtg atcgaacagg
     4921 aacgggtcag cgccgcgccg ttttgcgaaa agctgaacat ccccggaaac agcgaagtgt
     4981 tccatctctg ccgggtgatg tatctcgata aagagccgct gtttattgat agttcatgga
     5041 tcccgctgtc gcgttatcct gactttgatg agatttacgt cgaaggaagc tccacctatc
     5101 agttatttca ggagcgtttt gacacgcgag tggtcagcga caaaaagacc atcgatatct
     5161 ttgccgccac ccgcccacag gcaaaatggc tgaaatgcga actgggcgaa ccgttgtttc
     5221 gcatcagcaa aatcgccttt gatcagaatg acaaaccggt gcacgtctcc gaactcttct
     5281 gccgcgccaa tcgcatcacc ttaactattg ataataaaag acattaaccg taggccggat
     5341 aagatgcgcc agcatcgcat ccggcgatgc tggcgcgttg aattttacat cccgtacgtt
     5401 cccctcaccc taaccctctc cccaaagggg ctaggggacc gttcagtacg gaatttagct
     5461 tgacggtgct atccagcatt aaatactggc aatactctca cgcaaaatcc gcagcaccgt
     5521 ctcttcaccg ctgcgattcg ggttaatgcg aatcgcgcaa tgttcgagct gtggattcgc
     5581 ctggcgaaac gtcccggaaa gtcgataaaa aagtggcggg atttcatatt tcgactctgc
     5641 accgaccggg taaggtaagg caccgcgttt ctgcgcttct tccagcaccc tggcggcaat
     5701 cggctgatga aactccacaa tcaatacctt cgactgcgca ttagcaatga cggcgctttt
     5761 cacttccggc actgcaccac cgttgagcaa agccagcaac cgttcagata cccccgcctg
     5821 caccgcgtgc atcactggcg caaacaccag accacgcaag acttccagcg cctgagcgcc
     5881 ctggatctgg ctaccgcctg agtaaagcgt ggcgcgaata cggttgataa catcagcatc
     5941 gccgaccact gcaccaacac cctctggccc aaatagcttg aagcaggaaa atgtcgagac
     6001 attcgcgccg cattcacagc cgattcgcgc caccttcatc accgcatagt tgtcatcggt
     6061 taacgctgga acacctgccg cgcgcaacgt tgccagcaca tctgccagca cgtagctgtc
     6121 ctgcggctgc tggcgcgtat gctgcaccag cgccgcatcc ggttgttgct cgtcgacgac
     6181 ctgcttcagt gccgacaggt cattgaaatc aacagtaata agcgtcagcc ccatctgctc
     6241 aataataacc cgtgtcgtcg ggtaaacagg cgcgtcatgc accagaagac gctgccccgg
     6301 tttgagcaaa gccgccagcc cggcgcgaat cgcgccagtc cccgcgccct gcaccagcgc
     6361 cgcagcctgt gcgtgaaatg catcagccag cacctgttcc acacgctggg taacgcgcgg
     6421 ttgattcagt cctggcgtta agcctaaatc accgccagta agaaactcgc tgccggggaa
     6481 atggcgacaa atgctatcca ccagcgcaaa ctgcttttgc tgcgcctcaa taatcgtcag
     6541 gctttgcaga ggaaacgtct tcataggtgc cttccttaag ccggaacgaa caggccaagc
     6601 cagtaaagaa cattcagcag aataccggtg atcattacgg ccacgaccgg tgccgccatt
     6661 ttctgtaccg gacggcccag ggattcgttg aggaagtaaa tggcaaccgc gatagagaat
     6721 ccggtataac ccgccatttt aattgctgcg aaaatcgaac cgaccagcag cgccacttcc
     6781 atcagcatat tcatggcgtt acggatgtta tccgacgcat tacgcaccga cggatagcgc
     6841 cccagccatt tgccgatcga acgaagtaac aggacttctg ccgaaataac cactgcgcct
     6901 aataccgccg caaccatcgg attcggcgag agatagccca ccgcataaac aaaggtaaag
     6961 cccgcaactg catatacgcc ggtggctaac gcggtggtgg caatcaacgg cacaaaaccc
     7021 agtccgcgca taaactccgc cagagcagcc tggttgatca gcgtttgcga ttgttccggc
     7081 gttacgcctg cggagtacgc tttctccaga gtgaagatcg acacttcact gccggcgaaa
     7141 atcttcatac tggcaacggc ggcaatcaat gctcccacga tggcgatata gggtaagttt
     7201 ttgataatcc gcgacgtgcg ctcttcaaac accgaaagcc cgctggcatc gatgccattt
     7261 tcatcacgat gacgcaggtc gtgagttatc gcgattccca gcagcatcac catgccaata
     7321 aagatttcga tggattcagg gttaagatgt gggaaatagc gcacgactac cacacgggtc
     7381 atcagaacaa ccacggcggc gaccagactt tgcttccagc caaactggta gaaaatcgcc
     7441 accaacggga agagtgcaaa agcagaaacc accggcgagc ttaattcccc gaggctacct
     7501 aatacatcca ccggcagcgc ggtcagcagc tggtttactg gcaacaggca agtaaggatc
     7561 aacacgcccc agatagcgcc aagaccaaac gccatcaggc tgtttatcgc cagtacgccg
     7621 aggatatcgg taggaagaaa gagtaaccat gcgtttagca gcccggtttt cagggtaaaa
     7681 gagatcccca ccgaggccac aaaaccaatg ctcagaccaa aagcgatact ccccgcctcg
     7741 cgacggttca tatagccttc aatcagttgc ggcaggatcg ggcggatgcc gtcatgaaaa
     7801 acagccgccg agcgatgcgc cagaagcgat gtcatacccg tcaggcacgc caccacgata
     7861 atctgaatat acagatccat agtcgtgcct tttatttcag atggttaatc agcatcggaa
     7921 tcgcgtgttc gacgtgctca acggaaaggc caaacgccac tttcccttca gcgatcattt
     7981 tggcgatatg ctcgtctttc gctttaatgc ctggtttggc aatggtgcaa cttttgttat
     8041 agccaatcac cgcgatggca atcgacaatg ccgcgcccgc accggtatta cacgcgccga
     8101 tgtaataatc cagttgcccg gatttcactt tcattgccgc ttccatgtcg ttgtgaatga
     8161 aaacttcaaa actgccagga gccgtcgctt caatggtttt tttaatctgc tcacgctgta
     8221 agcctgcaac gccaatcttt ttcatgagtc tgtccttatt ggaaaaattg agagggagtt
     8281 tcacgtaaca tcacatccac atcggcctga ctgaatcctg actggcgcaa ttgcggaata
     8341 aaggtggtca ataaatagtc atagccataa ccaccattgg cttttaaatg ggagcggcgc
     8401 gtaatatcca tcgacagcat gacgcggttc agcaacccac ggtcacgtag tgcgtggagc
     8461 atcgcaatgc gcttttcgtc cgggtagtaa ctgttcttac caatggtgtc gaactgcacg
     8521 tacgcgccga gatcgatcat cttcaaaatg ttgtcgaggt tgtctttcag atcgcagtga
     8581 ccaacggtga cgcgcgaaag atcaactccg tgggcttgca acaaagccag ttgctccagc
     8641 cccatcgtgc tgaacgacgt atgcgtggag atcgggcgtc cggtctggtt atgtgccagc
     8701 gcggcggcaa taaacacctt ctcttccagc ggcgtaatct tcccttcgct ggagccgatc
     8761 tccgcgatga tcccggcttt cagttccgtg ccatcgatac cctgctcaat ttcatcgacc
     8821 atctcctgcg ccagttcctg cacgctgcgg gtcgccacat gttccgggaa aaacgcgtcc
     8881 tggtaataac cggtacaggc caccacgttg atccccgtct cgcgcattac atcgagcata
     8941 aattgcgcat tgcgccccat gtaacggttg gtcatctcaa tcacattacg cacgccccgg
     9001 gtcatcaggt cgttcatctc ctggcaaatg aacgcatact gatcaaggcg gcagtccacg
     9061 ttgtttttaa agccggagag atcaatatgc agatgctcat gggcgagggt gtaacccgtc
     9121 ggatcaaaac tcatagggtg tctcctgcaa accggaggga ggaaagaaaa gagcgaccat
     9181 tttgtggcgg tggcgcgcgg aaaaattcac acacggtagc ccccacatca gaaagcgtgg
     9241 tgcgcacacc gagctgcgta gcgaccagcc cttgctgata aaccagcaca ggcaccactt
     9301 cgcgggtatg gtggctgtga ccaatggtcg gatcgttgcc gtgatccgcc atcacgacca
     9361 ggcaatcatc tggctgcatc gcctcaacaa gccgggcaag gttacggtca acgacctgca
     9421 aacgttcggc ataacgtgcg acgtcttctg catgaccagc gaggtcggtt tcctggatat
     9481 tggtgcaaat aaacgccgcc ggatgggtgt taaattcgtc gagggtgata tccataatcc
     9541 gctggctatc caccaggttt tgccagctca cgccataagg attgctgacg atatctgcca
     9601 ccttacccac cagcacggtt ggcacgcccg cctcatacag tttttgcggc acctgcactt
     9661 ttttatcgac accgtagccc atatgcacga cctggaagcc gttgtcataa gcgccagaac
     9721 gcggcgcatt gataccaata aagcgccctt ctttgctttc tgcggcatcg agaatgcgtt
     9781 gactgtcggt taacaggcca ccaaatgtaa tgacccgacc gacctgtacc tgctcacgca
     9841 cgatacgacc aattttgatt gcgtcgtcaa aagagatcac agagagattg gcggtaatgt
     9901 tatagacctg gcctaaatcc gcctcgagat tatcgccaat cgcaaccgcc tgattgaccc
     9961 acagaaattg cagttcatcg ccacggcgct ccacctgcca accggcggat actaatgcct
    10021 gctcaacacg gtcaatcaca tcgcaaaaag gcatccgcag cggcggtaac gggcgcgtgc
    10081 ctaaaatttc ctgatgcccc ataaaggtat cgccaccttc atgttgcagc tctgccacgc
    10141 cccaggttgc ggaatctgac ggctgcatat cgcctggcgc ataacccaat gcgttgatta
    10201 gccccagctt ctccagcgtt ggtagctgca aatgcggcaa ctggctcagg atgtgaccac
    10261 atgtattcgc tcccgcatct tgcggacgca ccagcgtgac atctttcatt gcccctacgc
    10321 caaagctatc aatcactaac accacaaatc gcgccattac gcccccagag agtttccaag
    10381 actgtcataa cgaccgacga tttccggttc gccacgatga ataccggaca ccagcaccac
    10441 atcactacga gtgacaaaaa tctgcgtgcg aaaacagagc accactgcac tgcttaccgg
    10501 aaactcgcct gccagcggca gggtgtagtc gatactgctg tcatccacag ttttgagatt
    10561 ggtttcagta atcttttgat tttctggcgt aaacaccaac gcatgttgcg catgaccacg
    10621 acgatagtaa ccgccgccgt agcagtagct gtcgccacgg aaatgatggg agatttcact
    10681 taaccagagc atcgcgatac gttcaggctg atcgccctgc tggtttgccg gaatagtgcc
    10741 cgtcagcgca tgaccgggtt cggcatgagt cacaccgtat tgcgccagta atggcagcga
    10801 agtgcagctg gtcgctgaag gcgcgttcag ttgctcaagt gcaataccag atttcgccag
    10861 ttgatcccgt gcctgtatca gcgtgtgaag attcggtgtc ggcaaaactt ttccgacagc
    10921 ctcatcccaa agcaggcaag ggaaatgggt aagtccggct aaatgcagcc ctggcagatt
    10981 ctggatttcg gcgacaatct ccggcaacac cttgagggca aaaccgctct cctggcccgg
    11041 ataaagaaaa tcatcgtcgc tataaacttt aagcagcaca gactggattc gcccggcctt
    11101 caccgccgcc gcagaaactt cccgcgcttt gtcgagagta aacacggtga tgacgtcggt
    11161 gccctgttca acggcgtcag caacctgatg acaagggatt tgtaccagat gcccctgatg
    11221 cgccaccggc aaaccagcgc ggcgcatgac tcgcgcctct ttgtaatcca ccgccacaat
    11281 gccgctatag cctaatgcca gcaatttttc cgccagccac ggattacgac caaactgctt
    11341 ggtcatcaga tacagttcaa tgccgtaaag ccgcgccgtc tcaatcagtc gcttaccgtt
    11401 ttccagtacc tgatccacgt cgatcaccca gctgtccgga gcgatctttc cctgctgcca
    11461 caggcttagt gcggcggaaa tcagcgccgg gttctggcgt ttcaatgctt ctacaaacat
    11521 cttgttgcct cgtttctgtt ccttgaatta aatattcatt tttttgaata tttaggttgt
    11581 acgacgtgga tttgtcggga gcttcaaacc tcttcgttag cagccatcca taatccataa
    11641 aggttggcca gcaaatagcc ttcttcacac gggttaactt ccagtgcgaa ttccttcaac
    11701 aacacctgat gcaattgcac aacggcttgc cagtggctgg attgcgccag ctctgccagc
    11761 aactcgttat ccagtggctc aatttcttca ccacggcgac tgcgcatcag tgcactcgcc
    11821 atatgtgtca tcgccatcgt tccttgctca ctgcgcaccg gcagatggca ctctgtttcc
    11881 agtacgttga cgacctgcat catgcctttg cagatgtcct tatcaataac gcctgcctcg
    11941 caaagcaggt tgagtctggt ttccatatca gatgcctttc gtttaataac tcggttcctg
    12001 ttccccactc actacccgtt gttgatgggc gagcagggcg tgtttatcta caacggcacg
    12061 cagcagttgt tgcattgggt aatcatcgac tcgcgtcagg accacggctt cggtggcctg
    12121 taaaaatcgc ggatcgtctg tgagcggcgt cgcctctaat cccagcatgg tcagttcgtt
    12181 ttccgccacc acgttccaga tcaccgcatc gacatcgcct ttaacaatgc gttgtaaact
    12241 ctcgtgataa gagagatcga ctcgttccac atcactaccg ccaaaaaaaa catcggtcat
    12301 gattttctga tccgccgaac ggttatccag ccccacgcgc ttcacgtttg cggactcgcc
    12361 tttacggtaa atcaactggt gctcgccaac gtaggtgtgc ggccccaact ccagcgcgag
    12421 gcataagcct ttttgcgtga gataactttc cgccgctagt cgcgaaacca ccgccatgtc
    12481 atacacgcca ttaagcagac actccacgcg aatatccgcg ccgcgcatat gcgcatagta
    12541 aaaaggaatg ccatcaaact gggctttcaa tccgctcgcc aggccttcgt acaaacgggt
    12601 atagggcaag ggcatcgcgc acaccacgtt gttgatatcc acatgactca gcagcgcttt
    12661 gttatccatc tcgaccagat aactgccatt gcgcccacgg cgttcaatcc gtatcgctcc
    12721 gcttgattcc agcgttttca acgcggcctg cgtcaggcca acggatgaac ggcattcatt
    12781 tgccagctca tctatggttt tcaatcggtt accgcacttt tcacccaaca aataacgggc
    12841 cagcgtcgtt atgacgacgc cttctttttt gataaacgtt cgacgcataa taaattttca
    12901 gtaaattgaa tatttatatc ttcaggaatt tgaagataag gcgcaacagc gagatgtgga
    12961 aacggcgagg cacttcacat tttttcggat tattgcaaat gaggataaaa aaaccgggtt
    13021 tccccggttt cagagtgatg ataaaagcaa aattgcctga tgcgctacgc ttatcaggcc
    13081 tacatttcct tgcaatatgt gcattacttt gtaggccgga taaggcgttc acgccgcatc
    13141 cggcatgaac aaagcgcaat ttgccagcaa tagtgaatta cggcttcgcc acaaaaccaa
    13201 tcgcttcgta taccgctttt agcgtacggg aagcgtgcgc gctggctttt tccgcgccat
    13261 ctttcatcac ttgttgcagg aaggcttcat cgttgcggaa acggtgatag cgttcctgca
    13321 attcagtcag cataccggaa acggcatcag ccacttcgcc tttcagatga ccatacatct
    13381 tgccttcgaa ctgtttttcc agttccggga tgctctggcc cgttaccgca gaaaggatat
    13441 ccagcaggtt ggaaacgccc gctttgttct gcacatcgta gcgaactacc ggcggctcgt
    13501 cggagtcagt gaccgcgcgt ttgattttct tcactaccga tttcggatct tccagcaggc
    13561 caataacgtt attgcggtta tcgtcagact tggacatctt cttggtcggc tccagcagcg
    13621 acattacgcg cgcgccagat ttcggaataa acggctccgg caccttaaag atctcgccat
    13681 acagcgcgtt gaaacgctgg gcaatatcgc ggctcagttc gaggtgctgt ttctggtctt
    13741 cacccaccgg taccagatta gtttgataca gcaggatgtc cgccgccatc agcaccggat
    13801 agtcaaacag accagcgttg atgttctcgg cataacgcgc agatttatct ttaaactgcg
    13861 tcatgcggct cagctcgccg aagtaggtat agcagttcag tgcccagcct aactgcgcat
    13921 gttccggtac gtgggactga acaaaaatgg tgcttttctc aggatcgata ccacatgcca
    13981 gatacaaggc cagcgtatcc agcgtcgctt tacgcagctt ctgtgcatcc tggcgcacgg
    14041 tgatcgcgtg ttggtcaacg atacagtaaa tgcaatggta gtcatcctgc atgtttaccc
    14101 actgacgcag cgcacccatg tagttaccaa tggtcaattc acctgagggc tgtgcgccac
    14161 taaaaacgat gggcttagtc atttttcgat tcctgatttt cgctatgcgg aagccctaat
    14221 gcgggcagaa ggtcatttat agactgataa attacatcag gctggctgag atcgatagct
    14281 tcgccgtagt tatatccgta ggttaagcca acggatgggc aacctgccgc ttttgccgcc
    14341 tgaatatcat tgcgtgagtc gccgacaaac agcatctgtt gtggggcaat tcccatccgc
    14401 tcagccacca gtaacagcgg gtccggatga ggttttttgt tttgcacatc atcgccgccg
    14461 ataaccacgc taaagtattt ggcaatatct aaggcttcga gcagcggcgc gacgaacggc
    14521 gtcggtttgt tggtgaccag gcctagcggc aggcctttag cctgcaacgc gcccaacgta
    14581 tcggcaacgt gcgggaacaa aaacgtcccc tcttcggcaa cctcgccata atagcgatcg
    14641 aacagtttac gcagaatacg tacctgttct tctgccggaa tgtcgtcatc aacgggcggt
    14701 ttacccattg ttttacgcag agtcgcacgt tcctgacgcg cccaggtcaa tgcgcgctcc
    14761 atcagaacat ctgcgccgtt accaatccag gtaataacgc gttcttcacc tgcgacgggt
    14821 aactccaacg catacagcgc catatctacc gcagcagcaa gaccgggagc actgtccacc
    14881 agcgtaccgt cgagatcaaa agcgacgccg cgaatatctt caaacttact catgacttac
    14941 ctttgccagt tcactgcgca tttcatcaat gactttttta tagtctggct ggtcgaagat
    15001 tgccgaaccg gcgacgaaca tatccgcgcc cgccgcagcg atttcgccaa tgttgttcac
    15061 cttcacgcca ccgtccactt ccagacgaat gtcaaagcca gactcgtcga tacgacgacg
    15121 tacttcgcgc agtttatcca gtgtttgagg aatgaaagac tgaccgccga aaccaggatt
    15181 gacggacatc agcaggatca catccagctt atccatcacg taatccagat agctcagagg
    15241 tgtcgccggg ttaaatacca gacccgcttt acagccattt tctttaatca gttgcagcgt
    15301 gcggtcaaca tgctcggagg cttctggatg aaaggtaatg atgctggcac cagcggcagc
    15361 gaaatcaggc acaatgcgat cgacgggttt caccatcaga tgtacgtcga taggggcggt
    15421 aatgccgtag ttacgcaagg atttcagcac cattggccca atcgtcagat tgggaacata
    15481 gtggttatcc atgacgtcaa aatgcacgac atcagcgcca gctgccaggg cttttgcggt
    15541 atcttcaccc aggcgggcaa aatcagccga cagaattgag ggggcaatca aatactgttt
    15601 catccgcttc tccttgagaa ttattttttc gcgggtgaaa cgactcctgg tttgtacaaa
    15661 gccagcagtt cgtccacctt tttacgtgtg ccgccgttgc tgcttatact gcgtcgaact
    15721 ttgacgacat gcaattttgc gcgctgatac cactcacgcg ttaacatcgt atcgtgattg
    15781 gagatcagca ctggaatatg gcgctcaacc agaccttcgg cgatctccgc cagatgcgct
    15841 tgttgttcaa gcgtaaaact gtttgtgtga tacgccgtaa agttggcggt cgcagacagc
    15901 ggtgcataag gcggatcgca atagacgacg gatgcatcat ctgcgcgcgc catgctatcg
    15961 gcgtaagact cacaatagaa aaaggcattc tgcgcttttt cagcgaagtg atacaactct
    16021 gcttccggga aatagggttt tttgtagcgg ccgaacggca cgttaaactc accgcgcaga
    16081 ttgtaacgac acaggccgtt gtaaccgtag cggttcaaat ataaaaacag taccgcccga
    16141 cggaacggat cctggctttt gttgaactct tcgcggaact gatagtaaac ctcggcgcaa
    16201 tttgtttcgg gaacaaacag ctcgcgtgcg gcctgtacgt actcatcagt acgcatcttc
    16261 acaatgttat agagactgat caggtcgcta ttgatatcgg caaggatata acgagaaaag
    16321 tcggtgttga gaaacaccga cccggcacct acaaaaggct caaccagaca ttcgcccttg
    16381 ggcaaatgcc gtttaatatc atcaagcagg ggatacttgc cccctgccca cttcaaaaaa
    16441 gcgcgatttt tcttcatgct gactaactaa ttacaccttc tccggctgtg gagaaagctc
    16501 cgacagcatc cagcgcttca ggcaactccc gcaacgataa accattgcgg gagataacct
    16561 tgattacttc agatcggcct gtacctgacg cagcggtttc gcccacgggt ttttggcctg
    16621 aacatctgct ggcaatgtag ataccgcttt tttcgcctct tctttcgaag cgtacacgcc
    16681 agaaaccagg acataccacg gctgaccatt acgcgtcgtt tcatagacaa cgtagttttt
    16741 cagattctct ttcttcgccc aaccgttcag gttgtcgtag ttagaggaac tgctcagctg
    16801 cagagtgtaa tggctggacg gtgccgattt caacgaacca acattacctg cggtcttccc
    16861 tccagcggct ggtgttgccg tggtttgcgc cggggatgcc gtctgtactg gagccgtagt
    16921 cgccgtctct tttggtgctg gcgtagaagt cgcagccggt gccttcgtgc tcgccacagg
    16981 ggcagctggt tcagtacgct tcggcgtctg tgctaccggc ttcggctccg ttttcacggt
    17041 tgcttgcgat tttttcggtt caatcactgc ctgctggcga gctggacgcg tagtggccgg
    17101 acgttcagcg gtttgcgttt tcgccgtttc acgcgatgca ttgccattgc gaacaggcgc
    17161 gacagtcgcg ggttcggtcg gcaatgtgga attgaccgcc acattgttca actgttgctg
    17221 attttgtggc tgggtcagcg cattgttcag gtcaccctgc acttcaacac gttgttgacc
    17281 atccgttgcc gccggggttt gcccttgagt cggcgtagaa gagatcggtg gcagagaaac
    17341 atcctgctga gtattttccg cagacgtggt tcccggcgct ggctgcaccc cattcgcctg
    17401 atcggtcgca ttgcctgcaa gatcaatact cttctcgcca gacgcggttt gatcgctgga
    17461 agaggtcgag ggggctttta gcgcagaacc gataccgatg atcaacagca gtagaaccag
    17521 aatgccgacg cccatcatca tatactgacg agaagcgggt ttactggctg cttttttgcg
    17581 cttacgcgga cgacgctcta cgcgctcttc atccacggtt tcatcttcgg attcgtcaat
    17641 ttcttcttcg atttccggtt cctcattgcg ctctttttgc gcacgagtcg gacggcgatc
    17701 gtcagtgtca tcaagttcaa tatcatcaaa attgatctgc ggttcgccac gttcagtacg
    17761 ctcagaacgt tcagaagatt gacgagaacg accagtacga cgatcgctgg gatcgggttt
    17821 cagctcgtct tctggtttga attcatccat ttaacacccc actaaaaggt taatgcttac
    17881 cacgttgcaa ttaacctgaa gctaatagac cgcttgataa gcggcctgac ctttcttgtt
    17941 gttacgctga ttgacaatcg gcaatggcgt taagaacaag ctcgtgcgaa acgccgctgc
    18001 gaacttcact cttaccaatt gccaacggaa gaattaagcg catctctccc gcaaggactt
    18061 tcttgtcacg cagcatatgc ggtaaatacg cctgcgcgga catttcgcgc ggcccattga
    18121 ccggtaaccc agcccgcgtg agcagggtta taatacgctg cgtttcggca gaactaaact
    18181 gcccgagacg ttccgacgtc cgcgccgcca tcaccatacc cgcagcgacc gcttcaccat
    18241 gtaaccaatt gccatacccc atttcagctt caatggcatg accaaaggtg tgtcccagat
    18301 tcagtaaagc acgtaacccg gtttcgcgct cgtcggcggc gacaacttct gccttcagtt
    18361 cacaacaacg gcgaatacag tacgccattg ccggaccgtc cagacgcaac aacgcatcca
    18421 gattctcttc cagccagttg aaaaacgcac cgtcaagaat aatgccgtat ttgatgactt
    18481 ctgccagccc cgacgctaac tcacgcgggg gaagcgtttt cagacagtcg agatccacca
    18541 ccactgaagc aggctggtag aacgcgccaa tcatgttttt accgagggga tggttgaccg
    18601 cagttttgcc gccaacggag gaatcgacct gcgacagtaa cgtcgtcggg acttgaatga
    18661 aacgaacacc gcgctgataa ctcgccgccg cgaagccggt cagatcgccc actacgccgc
    18721 cgccaagcgc caccagcgta gtatcgcgac catgcggctt ttgtaacaac gccgtaaaga
    18781 cggtatcgag tacagccagg cttttatact gctcgccgtc agggaggata acgctatcga
    18841 cgttaacacc cgcctgttca agtacgccgc ggaccttatc gagatacaga ggagccaggg
    18901 tttcgttggt gaccaacatg acctgctcgc ccgatttcag cggtaagaat gaagctggtt
    18961 cattaaacaa accagatgcg atggtaattg ggtaactacg ttccccgaga gtaacgacaa
    19021 tcctctccat aacgcgacat ccaccttaat tactgtaccc gcagacgagt gtatataaag
    19081 ccagaattag ttgctttcca gcatgtgaat aatctggtta gcgaccactt tagcgctttg
    19141 atcatcagta cgaatggtca cgtcggcaat ctcttcatac agcggattgc gttcattggc
    19201 caacgcttcc agaacttcac gtggcggtgt ttcaacgtgc agcaacgggc gttttttatc
    19261 acgctgcgtg cgtgcaagtt gcttttcgat ggtcgtttca agataaacga caacgccacg
    19321 agcggaaaga cggttacgcg tttcacggga tttcacagag ccgccgccag tagccagcac
    19381 aataccctgt ttctcggtca actcattgat gaccttttct tcgcgatcgc ggaagccttc
    19441 ttcgccttct aaatcgaaaa cccagcccac atcagctccg gttcgtttct caatctcttg
    19501 atcggaatcg taaaattcca tattgagttg ttgagctaac tggcgcccaa tagtgctttt
    19561 tccggcaccc ataggcccaa ccagaaagat attgcgtttc tctgccattt tttcggtact
    19621 actaagacta ttcgttaatg ataaacccgc ttcgctcaga gagcgccgca ggacatgaac
    19681 tgaaacctca taagatattg cgagagtcag actgaaaatt atctcaatac tccagcgggt
    19741 ttggcaactg aataaatcac ccatccctct gcatatctgg tcgctgcaag cgcgctgcct
    19801 tgctaccacc gctctggcga taaatcaccg ggtaagatta gcgtaaaaaa gacagcaaaa
    19861 tgccgcctga atgataaatc atcatcatgg aacgcaggca gcgaagagca ccaactctca
    19921 aatcggtact ccttgtatgc taaatacctg cgcgcgtcaa atagatgaaa cacgttcagc
    19981 gtaaaaacat taccgctttt acggctgttt actcactgga aaccagtcgt ggcgtgataa
    20041 acaccactaa ctcgcgtcgt tcatcttctt ttccgtcatg acgaaataat tgcccgaaca
    20101 agggaatgtc gccaagcaac ggtacgctat cctgacccga tttattttta cgggtaaaaa
    20161 tgccgcccag cgccaacgtt tctccgcttt tgacctcgac ctgcgtttcg atctcctgct
    20221 tatcaatcgc cagcacttcg ccatcagcct gctgtagcac ctgccccgga acgttctggc
    20281 tgatgtgtaa tttcagccgg atgcgacctt tttgtaacac cgtgggcgtg acctccatcc
    20341 ccaggacggc ctctttaaat tccaccgacg tcgcgccact ttccccgctg gaaacctgat
    20401 atggaatttc gctcccctgt ttaatgctgg caggctgaag atgtgaggcc agcagacgcg
    20461 gactagcgat aatatccagc tgctgttttt gttcgagcgc agaaagctca agatccagca
    20521 aacgtccgtt gatgcgccca atgttaaaac cgacatgcgt tgtcgccgtc gctacggaga
    20581 ggtcgttacc aagcgtggtg acttgcccaa cgccaccagc gtgttgcgca tcggccagcg
    20641 tccatttcac gcctaactca cgcaaacttt tttcattaat ggtgacaata tgcgccgaca
    20701 gctcaacctg cccgaccggc agatccattt gcgctaccca ctgttcaagt gcgcttaacg
    20761 ccgttttgtt atctcgtagc aaaaggcgat tggtgcgttt atcgacggtc atactccctt
    20821 tggcactcag tagcttctcc cccgctttcg ccagttctcc cgcgtcggcg tattgcaggg
    20881 ttatactgcg attttccagc ggcagatttg cctgcgcccg cgcctgctcc gcctcctggc
    20941 gggcgatatt gttattctgc caggcaatgg aatgcactga gagaatgttg ccctcctggc
    21001 gcgttatcag tccggcgctt ttcactacag tttgtagtgc ctgcttccag ggaacatctg
    21061 ttagatgtaa cgacaccgta ccgctgacgt ctggcgacac gaccaggttc aacttctcct
    21121 gttcagccag cgcctgcaac acctgagcta ccggaacgtc atccaccatc agcgtcactt
    21181 tttgcggctt tgccgcttgt acgccgggta tcagcatcaa cagtagtgcg gctatccatt
    21241 gcttcatttg tatctccttg ccgttgccac aaccattgtg gcggttcgca gtttgtcccg
    21301 gttcccagcg ttagtgcgtc tggcgtcagc tgtaaaattg tccagccgtt ttccagcaca
    21361 tcgttttgct gcacccgtcg ccatttcttt tgcccgtctt ttattacacc gatgatgtgc
    21421 tcgcctcgcc ctaccatccc ctgatagcgc cactggctaa gttcgctaat ccggcataga
    21481 tcttccggcg gtttaaaagg gtcacgcata ccggttaaaa ggcacaatgc aacacctgcc
    21541 aacaaccagc gtttaaccct catgcggcgt ctccagttgt agcgtgaaca aaagatcatc
    21601 accttccacg cttaacgaaa aacggctcac gctgacgttg cgctctgcca gccgtgtaaa
    21661 tgccgacggc actgcttccc acagcgtttt caacgccaac tcacctccct gcgcggatgg
    21721 atgccaggaa accagttgcg cgccggataa ctgaaaatcc agtggcgaaa agggcagcgt
    21781 tttttcctcg ctgaagggag tggtgtctac caggcgatac agtgcggccc actgctggtg
    21841 atgacttgcc cgtagtcgaa ttaatgcgtc gctctcttcc tggtgtgtcg atgacagaaa
    21901 aatgagcgta actaacatca gcaaccagac tgcccagcaa agctggcgga gacggggtga
    21961 tgtggcgaac caccagtcaa agaacatgtt catcgctaac cttccttgtt aactgatact
    22021 caaattgcca gcgtccctgc gcatcctgct gcgtggcccc ccgctgattg agatgaaaag
    22081 aagcatcctg gcggagtgac gtttccagtg cgtttaacgc ggtaatgctt gttgtaagcc
    22141 ccttgatctc cagcgttccc tgctgccagc ttatcgttgt cagccaggcg tgctcgggta
    22201 aaagcgccgc cagagcttcc agcgcagatt gccagtcgcg ggtaaattgt cgctggcgct
    22261 gccgttgcaa acgctgttcg cgtaactgct gttgctccag caaacgtggt ttcgttatct
    22321 gtaagctgtg ggcgagttgt tgttccccct gaagcaaaac ggcgtctatg cgcgcttcgg
    22381 cgctgcctgt cagacgcagt attagcgtta tcccgacggc cagcagcaga ggcgcaacga
    22441 acatcagcaa ccagaaacgc agaaaagcgg tccggcgttg ctgtcgccag ggcaaaaaat
    22501 taattggcgg gttcatcagt acgccttccc aagcgccagc cccagcgcga tggcaaagtc
    22561 tccaccaggc ggcggtagcg gcggctgacg aacagaaacg gcctcccagg gatcaaaacc
    22621 gccttcgcca catatcgcga cgctgtcggg atcaacggat aacgctgccg ccagctcctt
    22681 cgcgctagtc atccctaccg ccagtttgcg cccccagcta tagcgcgtcg cccacagcca
    22741 ctgttcgtta tcacgccagg ccagacattg ctgatgagaa ggtaaaaaag gcaggaattg
    22801 ctgtaatgca ctggcatccg gggtgatcgc gctcacatga acacgcaacc tttctgccag
    22861 agtaagtagc gttgccagct ctttgctttg cgcggcagtc acgttataag cggggctgag
    22921 tgagtcttcg ctgtaatcga agcgcaggga gtccggatcc atatccagct cgcgggccat
    22981 cgtcccagac agccaggcca tttgctcccg ctcaccgagg gacatcgacg ggcgcggaaa
    23041 tgaccgctgt aatgtgcgac tggcgggaaa cgccaacatg atgtgatggc gctgcggcag
    23101 ttcgcgactc cacggcaaca acgttttagc cagccgctgc gcatcaacaa tccgcccatc
    23161 tttgataatg tcgtgcgcca acggcaaccg ccaccagcgt tgcaaaaagc attcttttgc
    23221 gtcccgcacg atcgcaaccg ctaacgcttc ttgctgttgt aaatgcaaac caatttgcca
    23281 gatcttaaat gccattgtga tgatctcctt atcacccgtc actctgacgg gtatatcaat
    23341 gcgtctggct tgcctttata ctaccgcgcg tttgtttata aactgcccaa atgaaactaa
    23401 atgggaaatt tccagtgaag ttcgtaaagt attttttgat ccttgcagtc tgttgcattc
    23461 tgctgggagc aggctcgatt tatggcctat accgctacat cgagccacaa ctgccggatg
    23521 tggcgacatt aaaagatgtt cgcctgcaaa ttccgatgca gatttacagc gccgatggcg
    23581 agctgattgc tcaatacggt gagaaacgtc gtattccggt tacgttggat caaatcccac
    23641 cggagatggt gaaagccttt atcgcgacag aagacagccg cttctacgag catcacggcg
    23701 ttgacccggt ggggatcttc cgtgcagcaa gcgtggcgct gttctccggt cacgcgtcac
    23761 aaggggcaag taccattacc cagcagctgg cgagaaactt cttcctcagt ccagaacgca
    23821 cgctgatgcg taagattaag gaagtcttcc tcgcgattcg cattgaacag ctgctgacga
    23881 aagacgagat cctcgagctt tatctgaaca agatttacct tggttaccgc gcctatggtg
    23941 tcggtgctgc ggcacaagtc tatttcggaa aaacggtcga ccaactgacg ctgaacgaaa
    24001 tggcggtgat agccgggctg ccgaaagcgc cttccacctt caacccgctc tactcgatgg
    24061 atcgtgccgt cgcgcggcgt aacgtcgtgc tgtcgcggat gctggatgaa gggtatatca
    24121 cccaacaaca gttcgatcag acacgcactg aggcgattaa cgctaactat cacgcgccgg
    24181 agattgcttt ctctgcgccg tacctgagcg aaatggtgcg ccaggagatg tataaccgtt
    24241 atggcgaaag tgcctatgaa gacggttatc gcatttacac caccatcacc cgcaaagtgc
    24301 agcaggccgc gcagcaggcg gtacgtaata acgtgctgga ctacgacatg cgccacggct
    24361 atcgcggccc ggcaaatgtg ctgtggaaag tgggcgagtc ggcgtgggat aacaacaaga
    24421 ttaccgatac gctgaaggcg ctgccgacct atggtccgct gctgcctgcc gcagtcacca
    24481 gcgccaatcc tcaggaagcg acggcaatgc tggcggacgg gtcgactgtc gcattgagta
    24541 tggacggcgt tcgctgggcg cgtccttacc gttcggatac tcagcaggga ccgacgccgc
    24601 gtaaagtgac cgatgttctg caaacgggac agcaaatctg ggttcgtcag gtcggcgatg
    24661 catggtggct ggcacaagtg ccggaagtga actcggcgct ggtgtcgatc aatccgcaaa
    24721 atggtgccgt tatggcgctg gtcggtggct ttgatttcaa tcagagcaag tttaaccgcg
    24781 ccacccaggc actgcgtcag gtgggttcca acatcaaacc gttcctctac accgcggcga
    24841 tggataaagg tctgacgctg gcaagtatgt tgaacgatgt gccaatttcc cgctgggatg
    24901 caggtgccgg ttctgactgg cagccgaaga actcaccacc gcagtatgcc ggtccaattc
    24961 gcttacgtca ggggctgggt cagtcgaaaa acgtggtgat ggtacgcgca atgcgggcga
    25021 tgggcgtcga ctacgctgca gaatatctgc aacgcttcgg cttcccggcg caaaacattg
    25081 tccacaccga atcgctggcg ctgggttcag cgtcctttac cccaatgcag gtggcgcgtg
    25141 gctacgcggt catggcgaac ggcggcttcc tggtggaccc gtggtttatc agcaaaattg
    25201 aaaacgatca gggcggcgtg attttcgaag cgaaaccgaa agtagcttgc ccggaatgtg
    25261 atattccggt gatttacggt gatacgcaga aatcgaacgt gctggaaaat aacgatgttg
    25321 aagatgtcgc tatctcccgc gagcagcaga atgtttctgt accaatgccg cagctggagc
    25381 aggctaatca ggcgttagtg gcgaagactg gcgcgcagga gtacgcaccg cacgtcatca
    25441 acactccgct ggcattcctg attaagagtg ctttgaacac caatatcttt ggtgagccag
    25501 gctggcaggg taccggctgg cgtgcaggtc gtgatttgca gcgtcgcgat atcggcggga
    25561 aaaccggtac cactaacagt tcgaaagatg cgtggttctc gggttacggt ccgggtgttg
    25621 tgacctcggt ctggattggc tttgatgatc accgtcgtaa tctcggtcat acaacggctt
    25681 ccggagcgat taaagatcag atctcaggtt acgaaggcgg tgccaagagt gcccagcctg
    25741 catgggacgc ttatatgaaa gccgttcttg aaggtgtgcc ggagcagccg ctaacgccgc
    25801 caccgggtat tgtgacggtg aatatcgatc gcagcaccgg gcagttagct aatggtggca
    25861 acagccgcga agagtatttc atcgaaggta cgcagccgac acaacaagcg gtgcacgagg
    25921 tgggtacgac cattatcgat aatggcgaag cacaggaatt gttctgatta aaaaggcgct
    25981 tcggcgcctt tttcagtttg ctgacaaagt gcactttgtt tataccggat acggcgtgaa
    26041 cactttaccc gtctagcaac atctagaaaa atcaatatat tgcagaaact tcgtaagcct
    26101 gataagcatt atgcatcagg cgaactttac gcatttacac tcgcccctgc cctttcaacc
    26161 attcgcgcac gaggaacagc gcgctgacat tacgcgcttc gttgaagtca gggtcttcca
    26221 gcaaatccat catatgcgcc agcggccagc gcacctgtgg tagcggctct ggctcatcgc
    26281 cttccagtga ttccgggtag agatcttgcg ctaccacgat attcattttg ctggaaaaat
    26341 aagacggtgc catgctgagc ttcttcaaaa aagtcagatc gttcgcacca aatccaacct
    26401 cttcttttag ctcgcggtta gcggcttcgt agacgctttc acccggatca attaatcctt
    26461 tcgaaaaacc taattcgtag gattcagttc ccactgcgta ttcgcggatc aggatcaggt
    26521 gatcgtccac aatcggcaca atcatcactg cttcccggtt ggttggacgc attcgttcat
    26581 aaacacgccg cacgccattg ctgaactcca gatccacgct ctcgacggta aacagtcggg
    26641 aacgggctac agtttcaaca ttcagaatgg tgggtttttg taatgatttg ctcatcgtgg
    26701 gatctatgct gtgaaatcag cggttattgt gcgatatcgg acacgctttc ggcaatgtga
    26761 attgcatgtt atttacattt atgtaactta ataaataatc gtcctcaaat caaattaaaa
    26821 gtcaataggt tgaaataact ccaggaattt gctgatattc cgccttcaga gggtttgcta
    26881 tgatcagcgg ttactgtgat gtgcttaatg atgctcaagt taaactccac gcttgccgat
    26941 agccaaccgc agaatcatgt attgtgtccg gtgcgactga ccacgcctga cagactaagt
    27001 aagatgggga aagcatgagc accattgtga tttttttagc tgctttgctg gcctgctcac
    27061 tacttgcggg atggctgata aaagtgcgat ccagacggcg tcagctgccc tggaccaacg
    27121 ccttcgcgga tgcgcaaacg cgtaaactca cacctgaaga acgtagcgcc gttgaaaatt
    27181 atcttgagag cctgacgcag gtattacagg tgcctggccc aacgggagcc agcgcggcac
    27241 cgatctctct ggcgctgaat gccgaaagca acaacgtcat gatgctgaca cacgctatca
    27301 cgcgttacgg catctctacc gacgatccga ataaatggcg ttactacctc gattcggtag
    27361 aagtccacct gccccctttc tgggaacagt acatcaacga tgagaatacc gttgaactga
    27421 ttcataccga ttcgctgccg ctggttattt cactcaacgg tcatacgctg caggagtaca
    27481 tgcaggaaac tcgcggctat gccttgcaac ctgttccgtc aacgcaggcg tcgattcgcg
    27541 gggaagagag tgagcaaatc gagctactca atattcgcaa agaaacgcat gaagaatatg
    27601 cgctgagtcg tccgcgcggg ctgcgtgaag cgttgctgat cgtcgcctcc ttcctgatgt
    27661 tctttttctg cctgattacc ccggatgtat ttgttccgtg gctggcaggc ggcgcgttac
    27721 tgctgctggg cgcaggtctg tgggggctat tcgcgccccc ggcaaaatcc tccctgcggg
    27781 aaattcattg tctgcgcggt acaccccgtc gttggggatt gtttggcgaa aacgatcagg
    27841 aacagatcaa caatatttca ctcggtatta tcgacctggt ctatcccgca cactggcagc
    27901 catacattgc tcaggatctc ggtcaacaaa ccgatatcga tatctatctc gaccgccatg
    27961 tagtgcgtca gggacgatat ctttcgctgc atgatgaagt aaaaaacttt ccgttacagc
    28021 actggctgcg cagtacgatt atcgctgcgg gctcgctgct ggtgctgttt atgctgttat
    28081 tctggatccc gctggatatg ccgctgaaat tcactctctc atggatgaaa ggcgcgcaga
    28141 ccattgaagc caccagcgta aaacaactgg ctgacgctgg cgtgcgggta ggcgatacat
    28201 tgcgtattag cggtacggga atgtgtaata ttcgcacttc cggaacctgg agcgcgaaaa
    28261 ccaattcacc ttttttaccg tttgactgct cgcagatcat ctggaacgac gcccgctcat
    28321 taccattacc agaatctgaa ctggtcaaca aagcgacggc attgaccgaa gcagttaatc
    28381 gccagctgca ccctaaaccg gaagatgaat ctcgcgtcag tgcctcatta cgttcagcga
    28441 ttcaaaaatc cggcatggta ttgcttgatg attttggcga cattgtactg aagacagcgg
    28501 atttatgttc tgccaaagat gactgtgtgc gactgaaaaa tgcgctggtc aatctcggca
    28561 acagtaaaga ctgggacgcg ctggtaaaac gcgccaacac cgggaagctc gatggcgtga
    28621 atgtgttatt acgcccggtg agtgcggaat cgctggataa cctggtggca acctccaccg
    28681 cgccgtttat cacgcatgaa acggcgcgag cggcacaatc actaaacagt ccggcccccg
    28741 gcggattcct gattgtcagc gacgaaggca gcgattttgt tgatcagccc tggccttcgg
    28801 catcacttta cgactacccg ccgcaagaac agtggaacgc tttccagaaa ctggcacaaa
    28861 tgctgatgca tacgccgttt aacgccgagg gtatcgtcac aaaaatcttc actgacgcca
    28921 atggtacgca gcatattggc cttcatccga tcccggatcg ttccggcctg tggcgctatc
    28981 tcagcaccac attgctgcta ctgacgatgc tgggtagcgc catttacaat ggcgtacagg
    29041 cctggcgtcg ttaccagcgt catcgcactc gcatgatgga gattcaggcc tattatgaaa
    29101 gctgcctgaa cccgcaactg atcacccctt cagaaagcct tatcgaataa cacgtttgcg
    29161 cggcaggtta tgctaccctg tcgcgcaaat tgcttcactc tggagatttc cctcatgcat
    29221 atcaacattg cctggcagga cgtagatacc gttctgctgg atatggacgg cacgttgctc
    29281 gacctcgcct tcgataacta tttctggcaa aagctggtgc ctgaaacatg gggcgcgaaa
    29341 aacggggtta cgccacagga agcgatggaa tatatgcgcc agcaatatca cgacgtacag
    29401 catacgctaa actggtactg tcttgattac tggagtgagc aactgggtct ggatatctgt
    29461 gcgatgacca ccgagatggg accgcgtgcc gtactgcgtg aagataccat tccgtttctt
    29521 gaggcactga aagccagcgg taagcagcga attttgctca ccaatgcgca tccgcacaac
    29581 ctgtcggtaa aacttgagca tacgggtctg gacgcacacc ttgatttatt actttccacc
    29641 cacacatttg gttatccgaa agaggatcag cggttatggc atgcagtggc cgaagctacg
    29701 ggtctgaaag ctgaaagaac gctgtttatt gatgacagcg aagcgattct cgatgctgcc
    29761 gcgcaatttg gtattcgtta ctgcctcggc gtgactaatc ctgattccgg gattgccgag
    29821 aaacagtatc aacgccatcc gtcactgaat gactaccgcc gcctgatccc ctcgctaatg
    29881 tgaaggagac gccatgaaag aaaaacctgc tgttgaggtt cgactggata aatggctatg
    29941 ggctgcccgt ttttataaaa cccgcgcgct ggcccgtgaa atgattgaag gcggtaaggt
    30001 gcattacaac gggcagcgca gcaagccgag caaaatcgtc gagctgaatg ccacgctcac
    30061 tctgcgccag ggaaatgacg aacgcacggt gattgtaaag gcgattactg aacagcgtcg
    30121 ccccgccagc gaggcagcct tgctgtatga agagactgcg gaaagtgtag agaaacgcga
    30181 aaaaatggcg ctggcacgta aacttaatgc cttaaccatg ccgcacccgg accgacgccc
    30241 ggacaaaaaa gagcgccgcg acctgttacg atttaaacac ggcgacagtg aataactgtc
    30301 acctgcaaga gagatgatta tgccgcaaca tgaccaatta catcgctatc tgtttgaaaa
    30361 ctttgccgtg cgcggcgaac tggtaaccgt ttcggaaacc ctgcaacaga tccttgagaa
    30421 ccacgattat ccgcagcccg ttaaaaacgt gctggcagaa ctgctggttg cgaccagcct
    30481 gttaaccgct acgctgaagt ttgatggtga tatcaccgta cagctgcagg gcgacggtcc
    30541 gatgaatctg gcggttatta acggtaacaa taaccagcag atgcgcggtg tggcgcgcgt
    30601 gcagggcgaa attccagaaa atgccgacct gaaaacgctg gtcggcaatg gttacgtggt
    30661 gatcaccatt accccgagcg aaggcgaacg ctatcagggc gtagttggtc tggaaggtga
    30721 taccctggcg gcctgcctgg aagattactt tatgcgttct gaacagctgc cgacgcgcct
    30781 gtttattcgc accggcgacg tagacggcaa accggctgca ggcggtatgt tgttgcaggt
    30841 aatgcctgcg caaaatgccc agcaggacga ctttgaccac ctggcgacgc taaccgaaac
    30901 catcaaaacc gaagaactgc tgaccttacc ggcaaacgaa gtgttgtggc gtttgtatca
    30961 cgaagaagag gtgacggttt acgatccgca ggatgtggag ttcaaatgca cctgctcgcg
    31021 tgaacgttgc gccgatgcgc tgaaaacgct gcctgatgaa gaagttgata gcatcctggc
    31081 ggaagatggc gaaattgaca tgcattgtga ttactgcggt aaccactatc tgttcaatgc
    31141 gatggatatt gctgaaatcc gcaacaacgc gtctccggca gatccgcaag ttcattaatg
    31201 agtatgtccg gcagagaggg ctctgccgga ttttacgact aatacacctg atgcgctttg
    31261 tcactcattc agattcacat tcagcctttc accatccact acaagatcgc gccacgcttt
    31321 aggcttcgtc cccagttttc catccttcgc agctgtgagt aatttttctt tcgttatttc
    31381 gttcggaagt aaactcatat caagcgcgtc ccattctttt ctgtctgagt caaagccata
    31441 gacaatgact ctgtcatcat taaaagcaaa caggatccgc tccgcctgac catcactatt
    31501 cagatcttgc tcgaccagga cgcaggcgtc tttttcaata caggtcatca cgttatagcg
    31561 atcctggatt aaggccgacc agaatgtcgc atcaggttta acagaaccag gggcaattaa
    31621 cacattatcg gctaatactt tttccgatac ctgttgctga agatgttgct ctccatctaa
    31681 cgccatcagc agatcccgcg cgcgtttcgg gtctttcata aacccggcat cgcttttcag
    31741 cgactcaagc gcagcacgtc catagcgacc gctctgctcg agcatgtaga tagttacctg
    31801 gtctgacgtg tttttgccgc tctgataacg cgccatatgg ctgttcacgc taatgcgcat
    31861 actgtccagc accggcgaat taagaagcac cagtatcacc aacaccaata acgaaaccgc
    31921 aaggttcact ttgccctgaa gaacaacggg attttgccct ttacgccaga cgatgctgac
    31981 aaaatacccc agcgaccaca ccagtaacac cagcaccgcc agcacgccct gcaagcggtc
    32041 gacagtccag ccgtactgag cgacccgcag ccataacgcc caggcggcga cgaacacata
    32101 cagcggagca accagcaaag cggttttaat caggcaacgc aacggccctg tccagggaag
    32161 tgacgctttt tgcggatcgc ggacaatagc cattaagatc aattgcaaaa aggccagcgt
    32221 caacagcaac ccggcggcgg agatgtggcg agaaatcgcg ctcaggcccg taaacggcag
    32281 ggtgatgata aacatcaggg ttagcaatga tactaacggc agcaaccccg tggcgattaa
    32341 cgtgaacaac ttttgaatag agtcgattaa acgtgactgt gttcgcgcga ggatcaccgc
    32401 cagcgcggta accagaccta acgtgagata aataaaccag tcggttgcaa aaaagagcgt
    32461 attgaaaaac gtgatgccaa caagtttaaa caactcactc cacagtaaaa gcaccagcca
    32521 cgtcaagcca ttggcgagaa aaatcaccag taatataagt acgttatgcc atactgactg
    32581 gtaaaaatag cgatagcggc tactgtcatt gcggatgcga aggctttgtt gtatccacgg
    32641 tagcaacaac attgccatca acagcagata gcaaccaaaa tcccaaagcg ccttttcagc
    32701 tctccagggg ttcatgccat cagtttgcca cttcagccag ccgctcatcc ccagcgtggc
    32761 aataaacact agcgccagcc atccccaaag gcgcttctgt ttaaaagaga tcacggagaa
    32821 gagtaaaacg gatgaaaacg cgacggtcgc aggcacgcca taaacaatcc aactgtgatt
    32881 ttttccggaa agccaggcga tcagcaggta gcaaaccaga ccctgaagta atccggtagc
    32941 aatcatcccc cagcgtgttg cgggtgaaag ttcaacgtta tccatgttgg ttatccagaa
    33001 tcaaaaggtg ggttaattat cgcatccggg cagtagtatt ttgctttttt cagaaaataa
    33061 tcaaaaaaag ttagcgtggt gaatcgatac tttaccggtt gaatttgcat caatttcatt
    33121 caggaatgcg attccactca caatattccc gccatataaa ccaagattta accttttgag
    33181 aacattttcc acacctaaaa tgctatttct gcgataatag caaccgtttc gtgacaggaa
    33241 tcacggagtt ttttgtcaaa tatgaatttc tccagatacg taaatctatg agccttgtcg
    33301 cggttaacac ccccaaaaag actttactat tcaggcaata catattggct aaggagcagt
    33361 gaaatgcgcg ttaacaatgg tttgaccccg caagaactcg aggcttatgg tatcagtgac
    33421 gtacatgata tcgtttacaa cccaagctac gacctgctgt atcaggaaga gctcgatccg
    33481 agcctgacag gttatgagcg cggggtgtta actaatctgg gtgccgttgc cgtcgatacc
    33541 gggatcttca ccggtcgttc accaaaagat aagtatatcg tccgtgacga taccactcgc
    33601 gatactttct ggtgggcaga caaaggcaaa ggtaagaacg acaacaaacc tctctctccg
    33661 gaaacctggc agcatctgaa aggcctggtg accaggcagc tttccggcaa acgtctgttc
    33721 gttgtcgacg ctttctgtgg tgcgaacccg gatactcgtc tttccgtccg tttcatcacc
    33781 gaagtggcct ggcaggcgca ttttgtcaaa aacatgttta ttcgcccgag cgatgaagaa
    33841 ctggcaggtt tcaaaccaga ctttatcgtt atgaacggcg cgaagtgcac taacccgcag
    33901 tggaaagaac agggtctcaa ctccgaaaac ttcgtggcgt ttaacctgac cgagcgcatg
    33961 cagctgattg gcggcacctg gtacggcggc gaaatgaaga aagggatgtt ctcgatgatg
    34021 aactacctgc tgccgctgaa aggtatcgct tctatgcact gctccgccaa cgttggtgag
    34081 aaaggcgatg ttgcggtgtt cttcggcctt tccggcaccg gtaaaaccac cctttccacc
    34141 gacccgaaac gtcgcctgat tggcgatgac gaacacggct gggacgatga cggcgtgttt
    34201 aacttcgaag gcggctgcta cgcaaaaact atcaagctgt cgaaagaagc ggaacctgaa
    34261 atctacaacg ctatccgtcg tgatgcgttg ctggaaaacg tcaccgtgcg tgaagatggc
    34321 actatcgact ttgatgatgg ttcaaaaacc gagaacaccc gcgtttctta tccgatctat
    34381 cacatcgata acattgttaa gccggtttcc aaagcgggcc acgcgactaa ggttatcttc
    34441 ctgactgctg atgctttcgg cgtgttgccg ccggtttctc gcctgactgc cgatcaaacc
    34501 cagtatcact tcctctctgg cttcaccgcc aaactggccg gtactgagcg tggcatcacc
    34561 gaaccgacgc caaccttctc cgcttgcttc ggcgcggcat tcctgtcgct gcacccgact
    34621 cagtacgcag aagtgctggt gaaacgtatg caggcggcgg gcgcgcaggc ttatctggtt
    34681 aacactggct ggaacggcac tggcaaacgt atctcgatta aagatacccg cgccattatc
    34741 gacgccatcc tcaacggttc gctggataat gcagaaacct tcactctgcc gatgtttaac
    34801 ctggcgatcc caaccgaact gccgggcgta gacacgaaga ttctcgatcc gcgtaacacc
    34861 tacgcttctc cggaacagtg gcaggaaaaa gccgaaaccc tggcgaaact gtttatcgac
    34921 aacttcgata aatacaccga cacccctgcg ggtgccgcgc tggtagcggc tggtccgaaa
    34981 ctgtaatgat ttgaagctgg agaatatcta tccagtatct tatagaaagc aaaacgggag
    35041 gcaccttcgc ctcccgttta tttacccttc ttttgtcgtg ccctgcgccc gcgttaccgg
    35101 cactggcagc caggcgcgaa tggaaagccc gccccgctcg ctggtgccaa gctccagcat
    35161 cccgttatgg ttatccacga tacgctgcac aattgccagc cctaatcccg tgccgctaat
    35221 ggtgcgcgca ctgtcgccgc ggacaaacgg ctggaacagg tgcttacgtt gttccggcgc
    35281 aattcccgga ccgtcatctt ccacctggaa ccaggcgcga ttcggctccg ttccgctgct
    35341 gactttgatc cagccattgc cataacgggc ggcgttgacc accatattcg ccaccgcgcg
    35401 tttgatcgac agcgggtgca ttttcacttc aatgctgccg gggtaaagcg cggtttcaat
    35461 ttcccgctca tagccacttt cggcagcaat cacctcaccg agtactgcat taagatccgc
    35521 catttccatc ggcatctcct gcccggtgcg caggtagtcg ataaactgct caatgatggc
    35581 gttgcactct tcgatatctt tattgatcga ttctgccaga tagccatcct gctcgctcat
    35641 catctcagtc gccaggcgaa tacgcgtcag cggcgtgcgc aagtcgtgac ttacccccgc
    35701 catcagcagc gtgcggtcat ccgccagttg cttaacacca gccgccatat ggttaaaggc
    35761 acgggtaacg gaacgcacct cggacgcgcc atactcacgc agcggcggcg gaataatccc
    35821 tttaccaacc tgcaaggctg cgtgttcgag atcgaccaac ggtcggttct ggatacgaat
    35881 aaacagccac gccccgccta tcgccaatag cataatcgcc agcgtatagc ggaacagcgg
    35941 agagaaatcg ccctgatgaa tttcggtcag cggcacgcgt acccagatat tgggcgacag
    36001 ccaggttttc agccagacga caggcgaact tttgttgacc tcaacgcgca cttccgtcgg
    36061 gccgcccagt tgctgcgcca tctgatggct taagaattca tagtgttgcg cccaacgcag
    36121 acctgcctct tcggcagcct cgttggagta gagagagatc cccagctcac ggtagatctc
    36181 ccgacggaaa gcgggaggca caaccaactg cgtgccgtcc tccagttgca gtttgtcggt
    36241 catcaacata cgcacttcgt acgcgaggac tttattaaac tgctggaggc tcggcaaaat
    36301 cgcgaagttc agcaccacca gataagtcgt caccaggctg gcgaacagca aggtgacgat
    36361 gagcaataac gtacgggcaa atgaacttcg tggcgagaag cgcaatcgcc tcatgcttta
    36421 gagccgtccg gtacaaagac gtagcccaga ccccagacgg tctgaatgta acgcggatgc
    36481 gctggatctt cttccaccat gcggcgcaga cgcgaaatct gcacgtcgat ggagcgttcc
    36541 attgcggaat attcacgacc acgggcaagg ttcatcagct tatcgcggga gagcggctca
    36601 cgcggatggc tgaccagtgc cttcagtacc gcaaactcac cgctggtgag cggcatcggc
    36661 tcgtcttcgc ggaacatttc gcgcgtaccg aggttaagtt tgaacttacc gaaagcaatt
    36721 accgcctctt cctgtgacgg tgcgcctggc agttcgttcg cctgacgacg cagcaccgca
    36781 cggatacggg ccagcagttc acgcgggtta aacggttttg gaatgtagtc gtcagcgcca
    36841 atctccaggc ctacgatacg gtccacttct tcccctttcg ccgtcaccat aatgatcggc
    36901 atcgggttgc tctgactacg aagacgtcgg caaatcgaca agccatcttc accaggtaac
    36961 attaaatcca gtaccataag atggaaagat tcacgagtca gcaggcgatc catctgttct
    37021 gcattagcga cgcttcgaac ctggaagcct tgttcggtga gataacgttc cagcagcgca
    37081 cgcaggcgca tgtcgtcatc gaccaccaga atcttgtagt tctcttgcat tgtttgtact
    37141 cccaaaggtt cgcaacaatt tgtaagcgtg tattcttaaa aaagctcacg ttcgtcacca
    37201 gctaaatctg gtatgaattt cagcctaaat tgttacaaag catattaaac agcagcttaa
    37261 gtatacaatt tattcggcga aacattattg attctgttga tatgatcacg ttatacccaa
    37321 tgtgcgcatt atcaaacaga caaagggaat caacgagatg aaaacgcccc tggttacccg
    37381 ggaagggtat gaaaaactca aacaagagct taattatctc tggcgtgaag aacgcccgga
    37441 ggtcacaaaa aaggtgacct gggccgcaag tctgggcgac cgcagcgaaa atgctgacta
    37501 tcagtataat aaaaagcgtc tgcgtgaaat cgaccgtcgc gtgcgctatc tcactaaatg
    37561 cctggaaaat ctcaaaatcg tcgattactc ccctcagcag gaaggcaaag tcttttttgg
    37621 cgcgtgggtg gagattgaaa acgacgatgg cgtgactcac cgtttccgta ttgtcggcta
    37681 cgatgaaatt tttggccgta aagattacat ctctatcgat tccccgatgg cccgcgcatt
    37741 gctgaaaaaa gaagtcggcg atctggcggt ggtgaatacc cctgccgggg aagcgagctg
    37801 gtatgttaat gctatcgagt acgtgaaacc gtaaggaaga gtcttaacct cctgccgatg
    37861 gctggcattt ttgccagcca gtccgtataa ctatcccctg atttttgatc cgaaaagatg
    37921 aactcaaacc atgatgaatg attcgttctg ccgcattatt gcgggtgaaa ttcaggcgcg
    37981 cccggaacag gttgacgctg ccgttcgcct gcttgacgaa gggaataccg tgccgtttat
    38041 cgcacgttat cgtaaggaaa tcaccggcgg tctggatgac acgcagctgc gtaatctgga
    38101 aacgcgtctg agctatctgc gcgagctgga agagagacgt caggcgatcc tcaagtccat
    38161 ttccgagcaa ggcaaactca ccgatgatct ggcgaaggcc atcaacgcca ccctaagcaa
    38221 aaccgaactc gaagacctct acctgcccta caaacctaaa cgccgcaccc gcgggcaaat
    38281 cgccattgaa gcagggcttg agccgttggc tgacctgctg tggagcgatc cgtcacacac
    38341 gccagaagtc gccgctgcac aatatgttga tgccgataaa ggcgtggcag ataccaaagc
    38401 cgcgctggat ggcgcgcgct atatcctgat ggaacggttt gccgaagatg ccgcgctgct
    38461 ggcgaaagtg cgtgattatc tgtggaagaa cgcgcatttg gtttctacgg tggtgagcgg
    38521 taaagaagag gaaggggcga aattccgcga ctatttcgat catcacgaac cgttgtccac
    38581 ggtgccttct caccgcgcgc tggcgatgtt ccgtgggcgt aacgaaggcg tactccagct
    38641 ttcgctgaat gccgatccgc agttcgatga gccgcccaaa gagagctatt gcgagcaaat
    38701 catcatggat caccttggcc tacgcctgaa caatgccccg gcggatagct ggcgcaaagg
    38761 cgtagtgagc tggacctggc gcatcaaggt gctgatgcat ctggaaaccg aactgatggg
    38821 taccgtgcgc gaacgcgcgg aagatgaagc aatcaacgtc tttgcccgta acctgcacga
    38881 tctgctgatg gcggcccctg ccggactgcg tgcaacgatg ggcctcgatc cgggtctgcg
    38941 tactggggta aaagtggcgg tggtcgatgc cactggcaaa ctggtagcga ccgataccat
    39001 ttacccgcac accggacaag ccgcaaaagc agcgatgacc gttgctgcct tgtgtgaaaa
    39061 acataacgtt gaactggtgg cgatcggcaa cggtacagct tcccgcgaaa ctgaacgttt
    39121 ctatctcgac gtgcagaagc agttcccgaa agtgaccgca cagaaagtga tcgtcagcga
    39181 agctggcgcg tcggtttact cagcttccga gctggcagcg caggagttcc cggatctcga
    39241 cgtttcgctg cgtggcgcgg tgtctatcgc ccgccgtttg caggatccgc tggcggagct
    39301 ggtgaaaatc gatccgaaat ctatcggcgt aggtcagtat cagcatgacg tcagccagac
    39361 gcaactggcc cgcaaactgg acgcagtagt agaagactgc gtaaacgccg ttggcgtcga
    39421 tctcaacact gcttctgttc cgctgttaac ccgcgtggcg ggcctgacgc gcatgatggc
    39481 gcaaaacatc gtggcctggc gcgatgagaa cggccagttc cagaaccgtc agcaactgct
    39541 gaaagtcagc cgtctggggc cgaaagcctt cgagcagtgc gcgggcttct tgcgcattaa
    39601 ccacggtgat aacccgctgg atgcttctac cgtccacccg gaagcctatc cggtggtgga
    39661 acgcattctg gcagcaacac agcaggcact gaaagatctg atgggtaaca gcagcgaact
    39721 gcgtaacctg aaagcgtctg actttactga tgaaaaattc ggtgtgccga cagtaactga
    39781 catcatcaaa gagctggaaa aaccgggtcg cgatccgcgt ccggaattta aaaccgctca
    39841 gtttgccgat ggcgtcgaga caatgaacga cctgcaaccg ggtatgatcc tcgaaggtgc
    39901 ggtgaccaac gtcaccaact ttggcgcgtt tgtcgatatc ggcgtgcatc aggacggcct
    39961 ggttcacatc tcttcattgt cgaacaagtt tgtggaagat ccgcataccg tggtgaaagc
    40021 gggcgacatt gtgaaggtga aagtgctgga agtggatctt cagcgtaaac gtatcgccct
    40081 gactatgcgt ctggacgagc agcctggcga aaccaacgcc cgtcgcggcg gcggtaatga
    40141 acgcccgcaa aacaaccgcc cggcagccaa accacgcggt cgtgaagcgc agcctgccgg
    40201 taatagcgcg atgatggatg cgctggcggc ggcaatgggc aaaaaacgtt aaacgcccgg
    40261 actggcccct aaacctgcgc gataaggcgt tcacgccgca tctggcaacg aacgccttgc
    40321 ctgacataaa agtgccggag aatatctccg gcctttttat tccacaacca aactcataat
    40381 atattccggc aatatttatc acttcattaa caactgaaac cttaattaaa cattagccag
    40441 tccgggtaat tcactattcg aattatattt tcgctgcgat ataaccttga gccacatcaa
    40501 cattgagtca gattattatt caaaccaaca ttcgcacaca ttttaagtat tgctgataga
    40561 aaccattctc attatcattg tgttgttgat tatttaatct ctcctttgtt ggcaaatcat
    40621 ctggtctcat gtcgctgtca aacgccccat gaggtagtta tccagttaat gagaaacaag
    40681 taggcaccta tgcaatacac tccagatact gcgtggaaaa tcactggctt ttcccgtgaa
    40741 atcagcccgg catatcgcca aaaactgctt tctcttggca tgctacctgg ctcctctttt
    40801 aatgtggtgc gcgtcgctcc actcggcgac cccattcata tcgaaacccg tcgtgtgagc
    40861 ctggtattac gcaaaaaaga tctggcctta ttagaagtgg aagcggtttc ctgttaatac
    40921 ggtgataaca acaatgaaaa aattaaccat aggcttaatt ggtaatccaa attctggcaa
    40981 gacaacctta tttaaccagc tcactggcgc acgtcagcgt gtaggtaact gggctggcgt
    41041 taccgtcgaa cgtaaagaag ggcaattctc caccaccgat catcaggtca cgctggtgga
    41101 cctgcccggc acctattctc tgaccaccat ctcatcgcag acctcgctcg atgagcaaat
    41161 cgcctgtcac tacattttga gtggcgacgc cgacctgctg attaacgtgg tggatgcgtc
    41221 taaccttgag cgtaacctgt acctgacgct acaactgctg gaactcggca ttccctgcat
    41281 tgtggcactg aacatgctcg acattgccga gaagcaaaat attcgtattg aaattgatgc
    41341 tctgtcggcg cgtctgggct gtccggtgat cccgctggtt tcaacccgtg gtcgcggtat
    41401 tgaagcgctc aagctggcga ttgatcgcta taaagctaac gagaatgtgg aactggtaca
    41461 ttacgcacag ccgctactca acgaagcaga ttcactggca aaagtgatgc cttccgacat
    41521 cccgctgaaa caacgtcgct ggctgggcct gcaaatgctg gaaggcgata tctacagccg
    41581 cgcctacgcc ggtgaagcgt cgcagcatct tgatgccgcc ctcgcccgtc tgcgtaatga
    41641 gatggacgat ccggcgctgc acattgccga tgcgcgttac cagtgcattg ctgccatctg
    41701 tgatgtggta agcaacaccc tgacggcaga acccagccgt ttcaccactg cggtagataa
    41761 aatcgtgctc aaccgtttcc tcggtctgcc gattttcctc tttgtgatgt acctgatgtt
    41821 cctgctggct atcaacatcg gcggggcgtt acagccgctg tttgatgtcg gctccgtggc
    41881 gctatttgtg catggtattc aatggattgg ctacacgctc cacttcccgg actggctgac
    41941 tatcttcctc gcccagggcc tgggtggcgg tattaacacc gtgctgccac tggtgccgca
    42001 gattggcatg atgtacctgt tcctctcctt ccttgaggac tccggctata tggcgcgtgc
    42061 ggcgtttgtg atggaccgcc tgatgcaggc gctgggcttg cctgggaaat cctttgtgcc
    42121 gctgatcgtc ggtttcggtt gtaacgtacc gtcggtaatg ggcgcacgta cgcttgatgc
    42181 accgcgtgaa cgtctgatga ccatcatgat ggcaccgttt atgtcctgcg gcgcgcgtct
    42241 ggctatcttc gcagtattcg cggctgcctt cttcgggcag aacggtgccc tggcggtttt
    42301 ctcgctgtat atgctgggta tcgtgatggc ggtgctgact ggcctgatgc tcaagtacac
    42361 catcatgcgc ggtgaagcga cgccgtttgt catggagctg ccggtctacc atgtaccaca
    42421 cgttaaaagc ctgattatcc agacctggca gcgtctgaaa ggcttcgttc tgcgtgctgg
    42481 taaagtgatt atcatcgtca gcattttcct gagcgccttc aacagcttct cgctgagcgg
    42541 aaaaatcgtc gataacatca acgactcggc gctggcttcc gtcagccggg tgatcacccc
    42601 agtcttcaag ccgattggcg tgcatgaaga taactggcag gcaacggttg gcctgtttac
    42661 aggtgcaatg gcgaaagaag tggtggtggg cacgctcaac accctctaca ccgcagaaaa
    42721 tattcaggac gaagagttca atccggcaga atttaacctc ggtgaagagc tgttcagcgc
    42781 ggtagatgaa acctggcaga gcctgaaaga caccttcagc cttagcgtac tgatgaatcc
    42841 cattgaagcg agcaaaggcg acggcgaaat gggtaccggg gcgatgggcg tgatggatca
    42901 gaaattcggt agcgcagctg ccgcatacag ctacctgatt ttcgtcctgc tgtatgtacc
    42961 atgtatctcg gtgatgggag ccatcgcccg tgaatcaagc cgtggctgga tgggcttctc
    43021 catcctgtgg gggctgaata tcgcttactc actggcaaca ttgttctatc aggtcgccag
    43081 ctacagtcag cacccaactt acagcctggt gtgcattctg gcggtaatcc tgtttaacat
    43141 cgtagttatc ggtctgctgc gccgcgcgcg tagccgggtg gatatcgaac tgctggcaac
    43201 ccgcaagtcg gtaagcagtt gctgcgcagc cagcaccacc ggtgattgcc attaatggct
    43261 tcacttattc aggtacgtga tttgctggcg ttacggggcc gtatggaagc gacccagata
    43321 agccagacat tgaacactcc gcagccaatg attaacgcca tgctgcaaca gctggaaagc
    43381 atgggcaaag ccgtgcggat tcaggaagaa cctgacggct gcctctctgg cagttgtaaa
    43441 agctgcccgg aaggcaaagc ctgtctgcgc gagtggtggg cgctgcgtta accatactcc
    43501 atcgcctgtt ttgaaaagcc cggtatgcgt ttgcatccgg gcttttttgc gtgcggcttt
    43561 ccataaaaat gcaattcttg cagcgcgacg taagttcctc tgaaagcgtc tcgcagggat
    43621 gaaaactggc taatgcacag atgtggagtg gcgcgtagag tcgcggcatt caaacaacag
    43681 gtgaaggaac gccatgagta aaaagcagag ttccacccca catgatgcgc tgttcaaact
    43741 ctttttacgc caaccggaga cggcacgcga ttttcttgcg tttcatttac cggcacccat
    43801 tcacgcgctc tgtgatatga aaaccctcaa gctggagtcg agcagcttta ttgatgacga
    43861 tctgcgtgaa agctattccg atgtgctgtg gtcggtgaaa acggaacaag gaccaggata
    43921 catctattgt ctgattgaac atcaaagcac ctcaaacaaa ctgatcgcat ttcgcatgat
    43981 gcgttacgct attgccgcaa tgcaaaatca ccttgatgct ggatacaaaa cgttgccgat
    44041 ggtggtgcca ttgttgtttt accacggtat tgaaagcccc tatgcctatt cgctgtgttg
    44101 gctggattgt ttcgccgatc ccaaactggc aaggcaactt tatgcctccg catttccgct
    44161 gattgatgtc accgtcatgc ctgatgatga aatcatgcag caccgacgta tggcgctgct
    44221 ggagttaatt caaaaacata ttcgtcaacg cgatctgatg gggctggtag agcaaatggc
    44281 ctgcttatta agtagtggat acgctaatga cagacaaatc aaagggctgt ttaattacat
    44341 actgcaaact ggcgacgctg tacgttttaa cgattttatc gacggcgttg ccgaacgttc
    44401 accgaaacac aaggagagtt taatgactat tgcggaaaga ttgcggcagg agggggaaca
    44461 atccaaagcc ctgcatatag ccaaaataat gcttgaatcc ggagtccctc ttacagacat
    44521 catgcgcttt accggggtgt cagaagaaga gttggctgcg gcgagccagt aaatttctgt
    44581 ctcgccattt caaaagttgc ctacaccctc tgcttcaacg ccaccagcag gtgacaaaac
    44641 tcgaccggat gcgaaataaa cggcgcatgg gccgctttgg cgaagatata tgattcgctg
    44701 tgaggccaaa gtttatccag catcggcacc actttgcgcg gcaccagacc gtcgagatag
    44761 ccatacaatc gcaaaaacgg catggacacg ttttgcagcg gctgacggag atcgaccgtt
    44821 ttcaggattt ccagcccgcc attaagcacg tcaacctccg gcatcggtaa cgccagaacg
    44881 gttttcttca acgcccgcgc atcctggcgc gccgtttcag tccccatggt ttgtaacgcc
    44941 aggaaccgct ccactgtacg ctgaaaatca tcactgagtt gctgctgaaa tcccgccagc
    45001 acgtccggtt ttatccccgg ccactcgtca cgagcactaa aacaaggtga cgacgccacg
    45061 gtgaccagcg cctgaacacg ctcgggatgg gttaacgcaa tctggcttgc caccagcccg
    45121 cccagactcc agcctaacca aatggcttta tcaggtgcct gttgcagcac ggcttcggcc
    45181 atatcagcaa gtgacagcgc accaaatccc cggctacgcc cgaagccggg caggtcaaca
    45241 aggtgcagcg taaaatgcga gctaagttcc tcgtcaatgc aacgccacac ttcggcattc
    45301 agtccccatc cgtgcagcag cacaagatga acattcccct gacctttggt ctgccaccag
    45361 atgttattca tccgctattg ttctcttttg acttacaagg atgaacatat gctaacagta
    45421 ccgggattat gctggctatg ccgaatgcca ctggcgttag gtcattgggg gatttgttcg
    45481 gtctgttcac gcgccacccg cacagataaa acgttatgcc cacaatgtgg attacccgcc
    45541 acacactccc atcttccctg cggtcgctgc ctgcaaaaac cgccgccctg gcaaagactg
    45601 gtcacagttg ccgactatgc gccgccgtta agtctgctca tccaccagct taaattttcc
    45661 cggcgcagtg aaatcgccag tgccctgtca cgtctgttac tattggaagt cttgcacgct
    45721 cgtcgcacca ccgggttgca attgccggat cgcatcatca gcgttccgtt atagcagcgg
    45781 cgtcactggc gtcggggatt taatcagagc gatttgctgt gtcagccgtt atcacgctgg
    45841 ttgcactgcc aatgggatag cgaagccgtc acacgtacac gggccactgc gacccagcat
    45901 tttctcagtg cccggctgcg caagcgcaac ctgaaaaatg cctttcgtct tgaattgccc
    45961 gtgcaaggtc gccatatggt gattgtggat gatgtcgtta ccaccggaag taccgtcgca
    46021 gagattgcgc agttgctttt acgcaatggt gcggcgactg tccaggtctg gtgcctttgt
    46081 cgaaccttgt agagcctcga tgatgggcgt attataacca actaaaatag tcaactatta
    46141 ggccattact atgatccgta tttccgatgc tgcacaagcg cactttgcca aactgctggc
    46201 aaatcaggaa gaagggacac aaatccgcgt atttgtgatt aaccctggca cgcctaacgc
    46261 tgaatgtggt gtttcttatt gtccgccgga tgccgtggaa gccaccgaca ctgccctgaa
    46321 atttgacctg ctgaccgcgt atgttgatga gttaagcgca ccatacctgg aagatgcaga
    46381 gatcgacttt gttaccgacc agttgggttc tcagttaacg ctgaaagcgc ctaacgccaa
    46441 aatgcgtaaa gtggcagacg atgcaccgct gatggagcgc gtggagtata tgctgcagtc
    46501 gcagatcaac ccacagcttg ctggtcacgg tggtcgcgtt tcgctgatgg aaatcaccga
    46561 agacggttac gccattctgc aatttggcgg cggctgtaac ggttgttcca tggtggatgt
    46621 gacgctgaaa gaagggatcg agaagcagct gctaaacgaa ttcccggagc tgaaaggtgt
    46681 gcgcgatctc accgaacacc agcgcggcga acactcctac tactaagtta tcccctcttt
    46741 tgtagattgc ccgatgcgac gctaaagcgt catttcgggc ctacattgaa catgtgccag
    46801 acgttggcgc agcattgctt tggcaaatcc ctcccttcgt ttccccgcga taatatgacc
    46861 aacctctcat aatttaaatt taccccgctc tggtgattct caaacgccag atgttacccg
    46921 tatcattcac atgggtacca aacatactcc tgacatctga ctacaataat tagttttagt
    46981 gggtatcagt cgtggtgccg caatatctct gttcccgatt gggataatta gagtttgtcg
    47041 tcagaaaatt gacgttaccc ataacaaatg aaaggccaag taaatcatgc cattagtcat
    47101 tgttgctatc ggtgtaatct tgttgttgct cctgatgatc cgcttcaaaa tgaacggctt
    47161 catcgctctc gtcctcgtgg cgcttgctgt tggattaatg caaggaatgc cgctggataa
    47221 agttattggc tccatcaaag ccggtgtcgg cgggacgctc ggtagccttg ccctgatcat
    47281 gggttttggc gcaatgctgg gcaaaatgct ggcagactgc ggtggcgcac aacgtatcgc
    47341 caccacgctg attgccaaat ttggtaaaaa acacatccag tgggcggtgg tactgaccgg
    47401 ttttaccgtt ggttttgccc tgttctatga agtgggcttt gtgctgatgc tgccgctggt
    47461 gtttaccatc gcggcttctg cgaatattcc actgctgtat gttggtgtac caatggcagc
    47521 tgcactgtct gtgacccacg gcttcctgcc accgcatccg ggtccgactg cgattgccac
    47581 cattttcaat gccgatatgg gtaaaaccct gctgtacggt actattctgg caatcccgac
    47641 cgtgattctc gccggtccgg tttacgctcg cgtgttgaaa ggtatcgata agccaattcc
    47701 ggaaggtctg tacagcgcga aaaccttcag cgaagaagag atgccgagct ttggcgtcag
    47761 cgtctggacc tctctggtgc cggtagtact gatggcgatg cgtgcgattg ccgaaatgat
    47821 cctgccgaaa ggtcacgctt tcctgccggt agcggagttc ctcggtgacc cggtaatggc
    47881 aacgctgatt gccgtgctga ttgcgatgtt cacctttggt ctaaaccgtg gtcgttcaat
    47941 ggatcagatt aacgacacgc tggtttcttc catcaaaatc attgcgatga tgctgttgat
    48001 catcggtggt ggcggtgcgt tcaagcaggt gctggtagac agcggcgtgg acaaatacat
    48061 tgcttccatg atgcacgaaa ccaacatttc tccgctgctg atggcctggt cgattgctgc
    48121 cgtactgcgt atcgcgctgg gttccgcaac cgttgcggca atcactgcgg gtggtatcgc
    48181 ggcaccgctg attgcaacga cgggtgttag cccggagctg atggttattg cggttggttc
    48241 cggtagtgtg attttctctc atgtgaacga tccgggcttc tggctgttca aagagtactt
    48301 taacctgact atcggcgaga ccatcaaatc ctggtcgatg ctggaaacca ttatctcggt
    48361 gtgcggtctg gtaggctgtc tgctgctgaa tatggtgatt tgataacact gactgccgga
    48421 tgcatcgcat ccggcatcaa ccgcactcta cttcttcttc gctgcagctc tgcgccgtct
    48481 gtccaaatcc ttcagcaact tgttcacgcc atcatcggca aacatcgtct caagcgttgc
    48541 ggagagcttg cgccgccagt ttttatactg gtaactggtg ccaggaatat tcaccggttc
    48601 ggccatatcc agccagtctt ccggctgtag tcctaacaga gcactgttac tgtcggcaat
    48661 gtagcgctgc aaaccacgat taagcgtcgg cgtcatcgac atcaacgatg ccttatgccc
    48721 ggcacgtttt ggcagacaac catatttatg cagtgcatcc agcagccctt gcttcgccag
    48781 ttcgcgatcc tgatacagac cgcgcagtac cacttcatcc ggatacagcc ccagggtttt
    48841 gcccagcgtt agatccccgc tctcccagta gccgcgtagc gtaggcaggt catgtgtcgc
    48901 cgcaaccgcc atcgactgct ccggatacgc tttcggtgca cggaacgtct tctcgtggtc
    48961 gttttcgaaa tagagcactt tgtaggagta cacaccgctg ctgcgcagct taccgacaat
    49021 ctctaccggt acggtaccga gatcttcacc aatcaccata cagcgatgac gtttactttc
    49081 gagtgccaga atcgaaagca gatcatccac cggatagtga acatacgcgc cctgatctgc
    49141 cgtctcgcca tacggtatcc accacaaacg cagcatcgac atcacatggt caattcgtaa
    49201 tgcgccgcag ttttgcatat tggcacgcaa cagctcgata aacggttcat aggcacgcgc
    49261 ggtaataata tgcgggtcca ttggcggtaa tccccagttc tgccccaacg ggccgaggat
    49321 atccggcggc gcgccaaccg acgctttcag gcaatacagt tcacggtcac accaggtttc
    49381 cgccccacct tccgctacgc caaccgccag atcacgatac aagccaatcg gcatttcata
    49441 gccctggctt atctcccagc aggcggcaaa ctggctgtaa gccagccact gcaaccagag
    49501 ataaaaatca acgtcatcac gatgttcttc gcagaactga cgcacttctg gtgaatccac
    49561 gttctgatac atctctggcc atgcaggcca gccccagcgc atttcgtcct ctttcacttg
    49621 ctgggcatgt agcgcatcaa aggctgcctg ccagaacagg ctgtcgcctt gatctgcaac
    49681 aaactggcga aacgcggcca tctgctcatc atcacgttgc gcgaaacctt tccacgccat
    49741 tcgtaatgct gtcattttta gggcggtaac cgtggagtaa tcgacccagt cggcatcgcg
    49801 cgcctgttgc agcgtctgtt gcgtggtcgg caactgccac caggcctgag cctcttcgct
    49861 aagatggaaa tcttcaacgg cgttgacgtc gatataaatc acattcagcc aacggcgaga
    49921 agacgggctg tatgggctgg cactctccgg atttgccgga tagagcgcat gaatcgggtt
    49981 caggccgatg aacgacccgc cacgttttgc cacatccacc agcatcgctt tgagatcgcc
    50041 aaaatcccca ataccccagt ttttttccga tcgcagcgta taaagctgga cgcaggcacc
    50101 ccacagcttt tgtttattca gcaacgcctg cggttcgtaa cagcgcttcg gggcgacaat
    50161 cacccggcaa tgcgcgcgct ggtcgtcctg ggtgagtgtc agcgtgtgat aaccttccgg
    50221 cagcttcgtc ggtagattga acgctttgcc ccccgttaca tggcctttgt actgtgttcc
    50281 ttcttcggtg gtcagcagcc agctatattc gccgctgccc tccaccacca tcggcatttt
    50341 tttgccgctg gtataaacca tgacattcgg gactggcgtt accgccactt tcgtggcggt
    50401 acgttgatgc atcgcgtcaa gcaaacgccg tttggtttcg gcgctaatcg actgcggttt
    50461 accgtgggca ttgatgtaat tggggctaat ccccgccgcc agcgcggcat tatccagacg
    50521 tttgctttcc atcgagcttc cttagcgttt tgcctgccag atacgagcct gataatcgcg
    50581 aatagagcga tccgagctaa acataccgca gcgggcggta ttgaggatcg ccgcgcgagt
    50641 ccaggcctcc tggtcgcggt acagcacatc cacctgcttt tgtgcctcaa cataggctgc
    50701 gaaatccgcc atcaccagat acggatcgcc gccttgtttg ccgatactgt gcagcatctg
    50761 gtcgaaggca tgcttatcgc cgtcgctgta tttaccgctt tccagctctt tcaatactgc
    50821 gtccagcacc ttatctttct tccgccattt caccgggtcg tagcctttgg ccagaattgc
    50881 cttcacttgt tccacggtat gaccaaaaat aaagatattt tcttcaccga ctttctcggc
    50941 gatttcaacg ttcgcaccat ccagcgtacc gacagtaagc gcaccattga gcgccagttt
    51001 catattgccg gtaccggaag cttctttacc tgcagtcgaa atttgttcgg agatatccgc
    51061 cgccgggatc agtttttccg ccgccgaaac gcaataatcc ggcaggaaca ccaccttcaa
    51121 cttatcgcca accagcggat cgttgttgat cacgtcagcc actttgttga tcgcaaagat
    51181 aatattcttc gccaggtagt agcccggtgc tgctttcgcg ccgaagagga agacgcgcgg
    51241 tacgcgatca gcctgcgggt tttcacgaat ttctttgtac aacgccagaa tatgcagcag
    51301 attcaggtgc tggcgtttgt actcatgcag acgtttgatc tgaatatcga aaatcgcctg
    51361 tggattgatc tcaataccgg tacgaacttt cacaaactcc gccagacgga ctttattcgc
    51421 ctgcttgatc tcgcgatatt gctgacggaa tttcgcatca tcagcgaatt tttccagatt
    51481 gatcagctga tcgagatcgt tagcccactc ttttttcagt gatttatcca acagagccgc
    51541 cagtgccggg ttgcactgtt tgatccagcg acgtggggta ataccgttgg tgacgttatg
    51601 gaatttgttc ggccatagct ggtgatattc cgggaacaga tctttcacca ccagatccga
    51661 gtgcagcgcc gcaacaccgt tcaccgcgaa accgccaacc acacacaggt tcgccatatg
    51721 cacttgtttg tcgtgcacca ccgccagttt ggcccacact ttttcatcgc ccggccaggt
    51781 tttctccacc agcgttttaa agcgagtatt aatttcgtta ataatctgca tgtggcgcgg
    51841 cagtaagcct ttcaccagtt tcacatccca gcgttccagc gcttctggca tcagggtatg
    51901 gttggtgtaa gcgaaagttt tgctggtaat ggcccaggcg tcatcccagc tcatctggtg
    51961 ctcatcgatc agcacgcgca gcagttctgg aatcgcgata gttgggtggg tatcgttcag
    52021 ctgaataact tcgtaatccg ccagttcgtg cagtttacgc cccgccagat gatggcgacg
    52081 caaaatatcc gctaccgaac aggcacactg gaagtactgc tgcatcaagc gcagcttttt
    52141 accggcagta tggttgtcgt ttggatagag aactttggtc agtttttccg cattgatgcc
    52201 ctgctgttcg gcacgcagga aatcaccgtc gttaaattta gtcagatcaa acggatgcgc
    52261 gtgcgtcgcc tgccacagac gcagcggctg cgccacgcca ttacgatagc cgacaacggg
    52321 gagatcccac gcttgaccgg taatggtaaa ctccggctcc cagcgtccgt ctttcgtcac
    52381 tttaccgcca atccctacct gcacatccag tgcttcgttg tggcggaacc acgggtagtt
    52441 actgcgatgc cagtcatccg gcgcttcaac ctgtttgcca tcgacaaaag actggcggaa
    52501 caaaccatat tgatagttca gaccgtaacc cgtcgcagac tgaccgacag ttgccattga
    52561 gtcgaggaag cacgccgcca gacgtcccag accaccgtta cccagcgccg ggtcgatctc
    52621 ttcttccagc aggtcagtca gattgatgtc ataagccttc aacgaatcct gtacatcctg
    52681 ataccagccg agattcaaca ggttgttgcc cgtcagacga ccaatcaaaa actccattga
    52741 gatgtagtta acatgtcgct gattcgccac cggtttggcg aatggctgag cacgcagcat
    52801 ttcggccagt gcttcactca ctgccagcca ccactggcga ggagtcattt cagccgcaga
    52861 atttaagcca taacgctgcc actgacgtga aagcgcttcc tgaaattgct tatcgttaaa
    52921 aataggttgt gacataggag ttccactttt cttagatttt caacacaacg ttatcgctag
    52981 tttgccaggc tcgatgttga ccttcctcat cctgcggggg attaggcagg gaggagttgc
    53041 ggggatgagc aaggaaatgt gatctcaacc acttaaagct agtgcaaacc acaggattag
    53101 cagcaaatca atgcaataca gcgcagaaaa tctgtatcta agtgcaaaaa atggccgttg
    53161 cgtattttca aaaagcggaa ggtaactcta taaattaagt aaaggagtga aacagtttca
    53221 taagtaaaat atccagtgtg ctccatctca ttcttaatag atttattaag atcatctttt
    53281 tagatggcac tttcatcagg aatgaagaag aaacccttgc ttaaatgaat ctgatgaaca
    53341 taagggaaac cagtattcac gctggatcag cgtcgtttta ggtgagttgt taataaagat
    53401 ttggaattgt gacacagtgc aaattcagac acataaaaaa acgtcatcgc ttgcattaga
    53461 aaggtttctg gccgacctta taaccattaa ttacgaagcg caaaaaaaat aatatttcct
    53521 cattttccac agtgaagtga ttaactatgc tgattccgtc aaaactcagt cgtccggttc
    53581 gactcgacca taccgtggtt cgtgagcgcc tgctggctaa actttccggc gcgaacaact
    53641 tccggctggc gctgatcacg agtcctgcgg gctacggaaa gaccaccctc atttcccagt
    53701 gggcggcagg caaaaacgat atcggctggt actcgctgga tgaaggtgat aaccagcaag
    53761 agcgtttcgc cagctatctc attgccgccg tgcagcaggc aaccaacggt cactgtgcga
    53821 tatgtgagac gatgacgcaa aaacggcaat atgccagcct gacgtcactc ttcgcccagc
    53881 ttttcattga gctggcggaa tggcatagcc cactttatct ggtcatcgat gactatcatc
    53941 tgatcactaa tccagtgatc cacgagtcaa tgcgcttctt tattcgccat caaccagaaa
    54001 atctcaccct ggtggtgttg tcacgcaacc ttccgcaact gggcattgcc aatctgcgtg
    54061 ttcgtgatca actgctggaa attggcagtc agcaactggc atttacccat caggaagcga
    54121 agcagttttt tgattgccgt ctgtcatcgc cgattgaagc cgcagaaagc agtcggattt
    54181 gcgatgacgt ttccggttgg gcgacggcac tacagctaat cgccctctcc gcccggcaga
    54241 atacccactc agcccataag tcggcacgcc gcctggcggg aatcaatgcc agccatcttt
    54301 cggattatct ggtcgatgag gttttggata acgtcgatct cgcaacgcgc cattttctgt
    54361 tgaaaagcgc cattttgcgc tcaatgaacg atgcactcat cacccgtgtg accggcgaag
    54421 aaaacgggca aatgcgcctc gaagagattg agcgtcaggg gctgttttta cagcggatgg
    54481 atgataccgg cgagtggttc tgctatcacc cgctgtttgg taacttcctg cgccagcgct
    54541 gccagtggga actggcggcg gagctgccgg aaatccaccg tgccgccgca gaaagctgga
    54601 tggcccaggg atttcccagc gaagcgattc atcatgcgct ggcggcaggc gatgcgctga
    54661 tgctgcgcga tattctgctt aatcacgcct ggagtctgtt caaccatagc gaactgtcgc
    54721 tgctggaaga gtcgcttaag gccctgccgt gggacagctt gctggaaaat ccgcagttgg
    54781 tgttattgca ggcgtggctg atgcaaagcc aacatcgcta cggcgaagtt aacaccctgc
    54841 tagcccgtgc tgaacatgaa atcaaggaca tcagagaaga caccatgcac gcagaattta
    54901 acgctctgcg cgcccaggtg gcgattaacg atggtaatcc ggatgaagcg gaacggctgg
    54961 caaaactggc actggaagag ctgccgccgg gctggttcta tagccgcatt gtggcaacct
    55021 cggtgctggg tgaagtgctg cactgcaaag gcgaattgac ccgctcactg gcgctaatgc
    55081 agcaaaccga acagatggca cgccagcacg atgtctggca ctacgctttg tggagtttaa
    55141 tccagcaaag tgaaattctg tttgcccaag ggttcctgca aaccgcgtgg gaaacgcagg
    55201 aaaaagcatt ccagctgatc aacgagcagc atctggaaca gctgccaatg catgagtttc
    55261 tggtgcgcat ccgagcacaa ctgttatggg cctgggcgcg gctggatgaa gccgaagcgt
    55321 cggcgcgtag cgggattgaa gtcttgtcgt cttatcagcc acagcaacag cttcagtgcc
    55381 tggcaatgtt gattcaatgc tcgctggccc gtggtgattt agataacgcc cgtagccagc
    55441 tgaaccgtct ggaaaatctg ctggggaatg gcaaatatca cagcgactgg atctctaacg
    55501 ccaacaaagt ccgggtgatt tactggcaaa tgaccggtga taaagccgcc gctgccaact
    55561 ggttgcgtca tacggctaaa ccggagtttg cgaacaacca cttcctgcaa ggtcaatggc
    55621 gcaacattgc ccgtgcacaa atcttgctgg gcgagtttga acctgcggaa attgttctcg
    55681 aagaactcaa tgaaaatgcc cggagtctgc ggttgatgag cgatctcaac cgtaacctgt
    55741 tgctgcttaa tcaactgtac tggcaggccg gacgtaaaag tgacgcccag cgcgtgttgc
    55801 tggacgcatt aaaactggcg aatcgcaccg gatttatcag ccattttgtc atcgaaggcg
    55861 aagcgatggc gcaacaactg cgtcagctga ttcagcttaa tacgctgccg gaactggaac
    55921 agcatcgcgc gcagcgtatt ctgcgagaaa tcaatcaaca tcatcggcat aaattcgccc
    55981 atttcgatga gaatttcgtt gaacgtctgc taaatcatcc tgaagtacct gaactgatcc
    56041 gcaccagccc gctgacgcaa cgtgaatggc aggtactggg gctgatctac tctggttaca
    56101 gcaatgagca aattgccgga gaactggaag tcgcggcaac caccatcaaa acgcatatcc
    56161 gcaatctgta tcagaaactc ggcgtggccc atcgccagga tgcggtacaa cacgcccagc
    56221 aattgctgaa gatgatgggg tacggcgtgt aagtttagcc ggataacgcg ccagatccgg
    56281 cttacatctc tgcatcattc aatgctcacc cgcgttacgc catctgtttc tatcaaacta
    56341 aaccgcaccg gcaagaaacg ctccaccacc gcgatattgg tcagcagatg gcatgaggga
    56401 tgggcgaccg taaattcccc cgcgcccgcc agcgccatcg gtagcaccag ttggtcagca
    56461 agatattccc ccaccgccgc cgtgcttgcc aggtagcgtt tcacctcttt caccaactgt
    56521 gccgcaacca cctcggcact gacgcgcttt tcaccgacga caaaaaagcg ttcggtgata
    56581 ttttcacttt cgacttcaag cgagacggta ttacccggcc cctggtcgcg cggcaggtta
    56641 tgaatattct gttcatgcag ggaaaaactc cccgccagtg tagcgatttc acgctcagca
    56701 acatggcgcg gcacaccagc taatagaact tctccacgca tctgcacaat gttcccgcgc
    56761 tcgccaagtt gcaaggtgtt aaacgatgcc acgggcgaga cttccgttgc caccacaccg
    56821 cctccggcag ggtaaaaacc gtggcgtaac agcgtggttt gctgatgaat tcccattttc
    56881 gccagcagcg gctccagcac ccggcggata aaatccgcag gtggcgccga cgggttatcg
    56941 gtaccgccgc tcacttcaac acgcgaaggt ccatcggcaa accacagcgc gggcagcaca
    57001 gtttgcagca ccagcgtaca acttccggcg ctaccgatag caaagcggta atcgccgccc
    57061 cgcacggtgc caggccggaa gagcagacgc tgcgatccca gctccgcacc ttccaccgtt
    57121 gccctacaaa tttccgcagc cgcttttacc gcggtcagat gctggcgcag cagccccggt
    57181 ttcgcccgtc cggctcgaat gccagtgatg gtaaatggct ggccggttat catcgacagg
    57241 ctcagcgccg agcgcaggat ctgtccgccg ccttcgccct gtgcgccatc cagcgcaatc
    57301 atccttttca tcatttatcc ttttacgcac accacctgac gcagggtgta gataacttcc
    57361 accagatcgc tttgtgccgc catcaccgca tcaatatctt tatacgccat cgggatttcg
    57421 tcgatcactt cggcatcttt acggcattcc acatgcgcgg tggcacgaat ttgatcttcc
    57481 acgctgaaca gttttttcgc tttagtacgg ctcattaccc gcccggcacc gtggctgcac
    57541 gaacagaacg actcttcatt tcccaaccca cggacgataa agcttttcgc tcccatcgaa
    57601 ccgggaataa ttccatattg accagcacgc gcagacaccg cgcctttacg cgtcacgtag
    57661 atctcttcac caaagtgctg ttctttttgc acatagttgt ggtgacagtt gatctcttcc
    57721 atcgccagcg tttgtggctg tctgaccgtt ttctgcgtaa tgctctgcaa tgccgttacc
    57781 acgttttcca tcatcgcatc gcggttaagg ctggcaaaaa gctgcgccca ggccacggct
    57841 ttcaggtaat catcaaagta ttccgtacct tccataaagt acgccagatc acgcgacggc
    57901 aacgtctcaa gcgtttcctg catctctttt tgtgccagat cgataaagta agtcccgatg
    57961 gcgttaccaa ttccgcgtga accggagtgc agcataatcc acacctgctc cgactcatca
    58021 aggcagattt caataaagtg gttaccggtt cccagcgttc ccaggtgttt atagttattg
    58081 gtattcagga aacggggata tttttgcgtt aaccactgat aaccggcttc aagctcagcc
    58141 catttcgcat cgacgttaac aggcgggttt tcccatgcgc ctttatcacg tttacaacgg
    58201 ccagtggtac gcccgtgcgg cacggccgtt tcaatcgcct gacgcagctc tgccaggttt
    58261 tcaggcaggt ctgccgccgt taacgcggta cgcagcgcgt tcattccaca gccaatatcc
    58321 acgcccaccg ccgccggaat aatcgcccct ttggtcggga tcacgctacc aatggtggaa
    58381 cctttaccca ggtgtacatc aggcattacc gcaatgtgtt tgaaaataaa cggcatcttc
    58441 gccgtattaa taagttgctg acgcgcatcg gcttctaccg gcacgccttt ggtccacatt
    58501 tttaccgggg cattttcagt ggtcagtaat tcgtaattca ttttgttttt ctcttttcgt
    58561 tgtttgctgt cgtgataatt gcaaccgtcg tgccagaaaa ttaaataagc agattaattt
    58621 tttaattcat tgtttttaaa taggttaaat ctttacgtcc gtaaacggag attttccaca
    58681 aaaccaattc accgataatg aaatatcgtc ttttataagg atatctaaga tgcgtaaaac
    58741 agtggctttt ggctttgtcg gtaccgtgct ggattatgcc gggcgcggca gtcagcgctg
    58801 gtcgaaatgg cttccgacac tctgtttatg ccagcaagaa tcgttggtca tcgatcgact
    58861 ggaattattg catgacgccc gctcgcgctc gctatttgaa acgcttaaac gcgatatcgc
    58921 cagcgtttcg ccagaaacag aagtggtgag cgttgagatt gaactgcata acccgtggga
    58981 tttcgaagag gtttacgctt gcctgcatga tttcgcccgt ggttacgagt ttcagccaga
    59041 aaaagaagac tatttaattc acattaccac cggcacccac gtcgcgcaga tttgctggtt
    59101 tctgctggcg gaagcgcgtt acctgcccgc ccggctgata caatcttcac ctccgcgcaa
    59161 aaaagaacag ccgcgcggcg caggtgaagt gacgattatc gatctcgatt taagccgtta
    59221 taacgccatc gccagccgct ttgccgagga acgccagcaa acgcttgatt ttcttaagtc
    59281 cggcattgcc acgcgtaacc cccacttcaa ccgcatgatt gagcagatcg aaaaagtggc
    59341 gatcaaatcc cgcgcgccga ttctgcttaa cggtccaacc ggcgcgggca agtcatttct
    59401 ggcgcgacgc atcttcgagt taaaacaggc gcggcatcag tttagcggcg cgtttgtgga
    59461 agtgaactgc gccaccctgc gcggcgatac cgccatgtcg acgctgtttg gtcatgtaaa
    59521 aggcgcgttt accggggcgc gggaatctcg tgaaggttta ttacgcagcg ccaacggcgg
    59581 aatgttgttt cttgatgaga ttggcgaact gggcgcagac gaacaagcaa tgctgctaaa
    59641 agccattgaa gagaaaacct tttacccgtt tggcagcgat cgccaggtga gcagtgattt
    59701 tcagcttatc gccggaacgg tgcgcgattt gcgccagctg gttgccgaag gcaaattccg
    59761 cgaagatctg tacgcgcgga tcaatctctg gaccttcacc ctgccggggc tacgccagcg
    59821 ccaggaagat attgaaccta acctggatta tgaagtggag cgccacgcct cactcaccgg
    59881 cgacagcgtg cgttttaaca ccgaagcgcg gcgtgcctgg ctggcctttg caacatctcc
    59941 tcaggcgaca tggcgcggta actttcgcga gctttctgcc agcgtcacgc ggatggcaac
    60001 ctttgccact agcggacgca tcactctgga cgtggttgaa gatgagataa accgtctgcg
    60061 ctataactgg caggagagcc gtccctccgc gcttacgcaa ctgctgggcg cagaggcaga
    60121 aaacatcgat ctcttcgacc gtatgcaact ggaacacgtt atcgctatct gccgccaggc
    60181 aaagtcgctt tctgctgccg gacgacagct ttttgatgtt tcgcgccagg gcaaagccag
    60241 cgtcaatgac gcggatcggc tacgcaaata cctggcgcgt tttggtctga cctgggaagc
    60301 attgcaggat cagcacagct ccagttgaat atggtggtcc gtcagcacct gcatcacgct
    60361 tactggcggc tgggcgtcgg tgtagacggc atctaccatg ctgatgctgc ccatattgac
    60421 catcgcgtta cggccaaatt tcgagtgatc gacaaccagc ataacgtggc gcgagttctc
    60481 aataatggcg cgtttggtgc gaacttcgtg gtaatcgaac tccagcagcg agccgtcgct
    60541 atcgatgccg cttatcccca gaatgccgaa atcaaggcgg aactgggaga taaaatcgag
    60601 cgtcgcttcg ccaatgatcc cgccatcgcg gctgcgtaat tcgccaccgg cgagaatgat
    60661 gcgaaaatct tcttttacca tcaacgtgtt agcaacgttg agattgttgg tgacaatgcg
    60721 caaattgctg tgattgagca gtgcgtgcgc taccgcttcc ggcgtggtgc cgatatcgat
    60781 aaacagcgtc gagccattgg ggatttgctc cgccactttg cgggcgatgc gctctttttc
    60841 ttcggtctgg gtggccttgc gatcgtgcca cggcgtgtta accgaactgg aaggcagcgc
    60901 cgcaccgcca tgatggcgca ggatcaggtt ttgctccgcc agctcattga ggtcgcggcg
    60961 aatagtctgc gggctgacgg agaaatgctc taccagctct tcggtactga cataaccctg
    61021 ctgtttaacc agttcgataa taccgttgtg acgttgtgtt tgtttcattt ataaatccct
    61081 ggaattattt tcgttttcgc gcattgagcg aatcaacaaa agccatcgct aaacccacgg
    61141 ctaacccggc gatgtgtgct ccgttcgcca tcgacatccc aaacaaatca aaccatccgg
    61201 cgacaatcca gatcagcgca aagataatta acccacgttg caggtaaatg ccactttgcg
    61261 gatcgcgttc gccacgtagc cagacgtagc ccatcagcgc atacaccacg ccagaaagcc
    61321 cgccaaacca cggcccgctg aatttttgct gcacatagcc gcttaacagg gcgctgataa
    61381 gcgtaatgac aattagctta ccgctaccga ggcgtttttc caccgcaccg ccgagatacc
    61441 accaccagag caggttaaag aggatatgca tcagcgagaa gtgcattaac gcgtgggtga
    61501 agtaacgcca gaactcaaat ttcagtgttg gatcgaatgg ccaggccagc cataacatca
    61561 cttcctgatc gccgagaatt tgcatggcaa taaacaccac cacgcaggcg atcatcatca
    61621 cccaggttac cggacctgcg cgttcacgca aggcggcaaa gaaaggataa cggcgataat
    61681 gcaggccact gccggtatgg cctgcctgcc agctcgccgc cagataacgc ggatctgccg
    61741 ggttttcgag aaaacgcgcc agctccgccc gtacgcgctc ggcctgggac tcatccgcca
    61801 gccagacatc gctttggtta tgttgttgaa tcgtgaggat aacaccctgc gtcgccatgt
    61861 aatcaacaaa cgcctgcgcc acgcgggggt tagcaaaaga ggtaatcatc aacatcgttg
    61921 ctgtcgctta ttccacacaa aaggggacag tataaagcgt tacgcgccgt acgccacctc
    61981 tgcgggaaac tgacgttgcc aggcttcaaa gccgccgtca atgctataga ccacatcgta
    62041 gccctgttgc agcagatact gcgccgcgcc tttgctgcta ttgccgtgat aacacatcac
    62101 catcaccgga gtgtcaaagt cgttatcacg cataaaagcg cccagcgtgt cgttggttaa
    62161 atggaaagcc tgcaccgcat gtcccattgc gaaactctgt ggatcacgaa tatcgaccag
    62221 caccgcctct ttttcctgca acttctggtg cgcgtcggca acgttaatac attcgaactg
    62281 atccatgcgt ctctctttct ttacaaacaa gtgggcaaat ttaccgcaca gtttacgtcg
    62341 aagcggcaga taaacgccat aatgttatac atatcactct aaaatgtttt ttcaatgtta
    62401 cctaaagcgc gattctttgc taatatgttc gataacgaac atttatgagc tttaacgaaa
    62461 gtgaatgagg gcagcatgga aaccaaagat ctgattgtga tagggggcgg catcaatggt
    62521 gctggtatcg cggcagacgc cgctggacgc ggtttatccg tgctgatgct ggaggcgcag
    62581 gatctcgctt gcgcgacctc ttccgccagt tcaaaactca ttcacggtgg cctgcgctac
    62641 cttgagcact atgaattccg cctggtcagc gaggcgctgg ctgaacgtga agtgctgctg
    62701 aaaatggccc cgcatatcgc cttcccgatg cgttttcgcc tgccacatcg tccgcatctg
    62761 cgcccggcgt ggatgattcg cattggtctg tttatgtacg atcatctggg taaacgcacc
    62821 agcttgccgg gatcaactgg tttgcgtttt ggcgcaaatt cagtgttaaa accggaaatt
    62881 aagcgcggat tcgaatattc tgactgttgg gtagacgacg cccgtctggt actcgccaac
    62941 gcccagatgg tggtgcgtaa aggcggcgaa gtgcttactc ggactcgcgc cacctctgct
    63001 cgccgcgaaa acggcctgtg gattgtggaa gcggaagata tcgataccgg caaaaaatat
    63061 agctggcaag cgcgcggctt ggttaacgcc accggcccgt gggtgaaaca gttcttcgac
    63121 gacgggatgc atctgccttc gccttatggc attcgcctga tcaaaggcag ccatattgtg
    63181 gtgccgcgcg tgcataccca gaagcaagcc tacattctgc aaaacgaaga taaacgtatt
    63241 gtgttcgtga tcccgtggat ggacgagttt tccatcatcg gcactaccga tgtcgagtac
    63301 aaaggcgatc cgaaagcggt gaagattgaa gagagtgaaa tcaattacct gctgaatgtg
    63361 tataacacgc actttaaaaa gcagttaagc cgtgacgata tcgtctggac ctactccggt
    63421 gtgcgtccgc tgtgtgatga tgagtccgac tcgccgcagg ctattacccg tgattacacc
    63481 cttgatattc atgatgaaaa tggcaaagca ccgctgctgt cggtattcgg cggtaagctg
    63541 accacctacc gaaaactggc ggaacatgcg ctggaaaaac taacgccgta ttatcagggt
    63601 attggcccgg catggacgaa agagagtgtg ctaccgggtg gcgccattga aggcgaccgc
    63661 gacgattatg ccgctcgcct gcgccgccgc tatccgttcc tgactgaatc gctggcgcgt
    63721 cattacgctc gcacttacgg cagcaacagc gagctgctgc tcggcaatgc gggaacggta
    63781 agcgatctcg gggaagattt cggtcatgag ttctacgaag cggagctgaa atacctggtg
    63841 gatcacgaat gggtccgccg cgccgacgac gccctgtggc gtcgcacaaa acaaggcatg
    63901 tggctaaatg cggatcaaca atctcgtgtg agtcagtggc tggtggagta tacgcagcag
    63961 aagctgtcgc tggcgtcgtg atttaatgta aggtggccag gtcagatttc aatctgacct
    64021 gagactgatg acaaaccaaa aaactgccag atgcgctacg cttatctggc ctacgttgtt
    64081 tatacaatat attgaatttg catgattttg taggcctgat aaggcgttcg cgccgcatca
    64141 ggcatgaaca aagcgccacg ttgtcaacaa tctgacctac tttttgattc agggaattac
    64201 cgcaaagccc acgccatcat acccaacgta acaaaactta agcacgttcc ccactcactc
    64261 aacttccgta tctggcatct atttttgctg caccactggc aataatatcc caacgcaaaa
    64321 ccaagctgtg cgcttattgg accgataaaa tccgtccacg gctgaggcca aaatggcgaa
    64381 aaattcaggg gtagccagac aacaagcaga aagaaattgc ggatactttc cagcaacgca
    64441 attttcgctg gcgcgatgta aaaaatgata ttccagagga tcaaccataa atagaccggt
    64501 gcgaatacca accaacaatg gagcctgctt ttcaattcag attgagttct acgagtaaat
    64561 tgccaaccaa cgcaacacat tacacagaag aaaagcgcat attcagccga taaccaaagt
    64621 tgtaattgca gaagtgaaat acgcctgatc atgagcggga ttgagaacag catttccccc
    64681 agaaaactca gcgtgataat acaagcagcc agcgcaacca ccatcggcca caccaacaac
    64741 ggcacaataa caaagccgag aaataacgga taaatataaa gtctgattat attcatcatt
    64801 attgtcctga tgattcgggg gatgcttcat acagcggcac ataccccaca tcctctacca
    64861 actgttgccc ctgctgactt ataaaccagt caacaaattt ctgcgtttcc ggcgtgggat
    64921 tttcacgcgt caccatatag gcatccacaa tgtatggata agtgccgttg cggatatttt
    64981 cctcggtagg cgcaacgcca tttatagcca aaagtttaat atgcttgcgc attttgagca
    65041 tattgtcatt catacgagtg acgtaatagt ggaaggtgta cccaatggaa gatggcgtgt
    65101 tttgatagtc ggcaacttca gtaattaaac cgcccataag atcaatcaca gtggattttt
    65161 tagctggcag cattggggtg tcttgcataa ccaacccctt cataatcgtt tggcttccag
    65221 aatcttcagg gcgctgccag acttttatat gttcgtcact accacctact ttattccagc
    65281 ggctagtttt gccgctaaaa atatctttaa cctggtgctc agagagagaa tttaccgggt
    65341 tattaatatc aacgataaag acaaaggctt cgcgggcgaa agcggtatat ttcagtttaa
    65401 cacccgcttc ttctgcacgt tttttctgtc cttccgacgg ttgtgcaaca aaaataaccg
    65461 tcgcggaacc tttaatcaga ctatcgtagg cttcagctgt tcgggtttgc tgcaaaccat
    65521 acgcctgcca ggccagcgag gttcgatcct tatcttttgg ctcgggtact aatttataat
    65581 acgcggaggc atacagcggg tataaggctg tagcgccgta cactctcggc catcttgacc
    65641 aatcatcgct gtaacgtaat gcaggttgcc cccgcagaga cgtcagtcca tggctctctt
    65701 cttccctaac gctgttatag tacccttccc ggtcgacaca tcccgccagt aacgccgtgc
    65761 ctaatagtaa aacaccggtg aatttattca tctcgctgat tcctttctct cgagcgttat
    65821 tccattgttg gatacagcgg cacatatccc acatcttcta ccagactctg cccctgcggc
    65881 gttaaaaacc attcgaccag tttttgtgtt tctgacgttg tattttctct cgtcaccata
    65941 aatgcatcga cgatgtacgc atatttgccg ttgcgaatgt tttccgccgt cggtgtaata
    66001 ccgttaatcg ctagcaattt tatattttta tcagcattca tttgcgtcgc gtaatagcgg
    66061 aaggtatagc ctattgcgtt gtttgtatta cggtattcgg caacgacttt aatcattccc
    66121 tccatcacgc ttgccacttt cgtttcctgc ggcgagatca tgcggacatt tttcatgacc
    66181 tgtgattgca tcactgtctg gctgccagag tcttccgggc gctgccaggt ctggatctcc
    66241 tgatcgttac cgccaacagc acgccaattg gtaattgcac cactgaaaat gtcacgcacc
    66301 tgttgttcag tcagggaatt aaccggatta tccgcattga cgatgaaaac gaatgcttca
    66361 cgggcaaagg gggtgtacag caaagtgaca ccagattcct ttgcacgttt tttttgccca
    66421 ccggaaggtt gcgccacgaa aataatatca gcatcgccct taacgatttt gttatatgct
    66481 tctggcgtac gagagttttc cagataatcc caaacgtgaa aatcctctga tattacactt
    66541 aatgcataaa atgcagaagc ataaattggg tacgcagccg tggcgccatc gattcgcggc
    66601 cagttttgcc taaactgaat ttgcggtttg ccacgcagcg gcgtcagtcg attattgagt
    66661 ttatcgggtc gccaggccca gatatcaata ttttcgcgta caccgacacc aggattttgc
    66721 tgcttatata aatgggcctg atacccggcg acaataccaa gtaagagaag tagaaacagg
    66781 agtaagttac ggcaacgtag tccggacgtg ccggataaag gatatctggt cggccaggcg
    66841 tatcccagcg aaaaactgat atgcgcccca acgggaataa tgaccatcca ccagtactca
    66901 ccacccacta atgagactaa aaaaacgccc aaccagggaa gcataaggtt tgccaaccaa
    66961 ccagctccca tctggcgaaa ttgataatct gccagatgta gcgtgattat ccagccaacc
    67021 agggttaata gcgcgggtaa gagaaaaaag cattgccgcc agacggctat ttctctgttt
    67081 tctggcgcgc tatcaccggc ccacacgcga ccagagaaaa atattattat cgcccccagc
    67141 gtcagcataa agacgttgag cagaaggtaa attgttggcg tgataaagat attacaaact
    67201 tcaataatac cggcaagtcc gacacccagc atggcaataa ccgccgccaa aaactgcgcc
    67261 agaatgggga tgccgaaaaa agtcattacc agcgaggtca aaatccattt tctgttttgc
    67321 attattcttt ccattctttc tgaatggcga aattatactc cccgagtccc cttgcccctt
    67381 ctggacactt ttccgaaatg atggcgaaaa aaacgggacc ctttggcccc gttctattta
    67441 ttagtgaaat tacaatctca ccggatcaat atgccagata tgatcggcgt actctttgat
    67501 agtacggtca gaagagaagt agcccatatt ggcaatgttc agcatcgctt ttgcggtcca
    67561 ctcttcctga agctcgtaga gttcatcgac tttatcctga caatcgacat agctgcgata
    67621 atccgccagt acctggtagt gatcgccgaa gttgatcagc gaatcaacca gatcgcgata
    67681 gcgacccgga tcttccggac tgaatacacc gctgccgatt tgcgtcagca cctgatgcag
    67741 ctcctcatct ttctcgtagt attcacgcgg tttgtagccc tgacgacgca gttcttccac
    67801 ttcttccgct gtgttaccaa aaataaagat attgtcagca ccgacatgat ccagcatctc
    67861 gacattcgca ccgtccaacg taccgatagt cagcgcaccg ttaagcgcaa acttcatgtt
    67921 actggtgccg gaagcttccg tccccgccag cgaaatctgt tcagacagat cggccgccgg
    67981 aatgatcaac tgcgccaggc taacgctgta gttcgggatg aacacgactt tcagcttgtc
    68041 gccaatctgc ggatcgttgt tgatcacttt cgctacgtca ttgatcaaat gaataatgtg
    68101 cttcgccatg taataggccg aagccgcctt accgccaaaa atattcacgc gcggtaccca
    68161 cttcgcatcc gggtcggcct tgatgcggtt atagcgggta atcacatgca acacattcat
    68221 caattgacgt ttgtattcgt gaatacgttt gatttgtaca tcgaacaacg cctttggatt
    68281 caccaccaca ttcagctgct gggcgatata ctctgccaga cgctttttgt tctccagctt
    68341 cgcctgatgc acagcgtgat taaccattgg gaaatcacag tgttgttgca gctcattaag
    68401 caggctaagg tcggtgcgcc agttacggcc caggtgttcg tccagcacgg ctgaaagcga
    68461 tgggttcgct accgctaacc agcgacgcgg cgtcacaccg ttggtgacgt tggtgaaacg
    68521 acccgggaag attttcgcaa agtcggcaaa caacgattgc accatcagat tagagtgcag
    68581 ttccgataca ccgttaactt tgtggctcac aacaaccgcc agccaggcca tacgcacacg
    68641 acgaccgttg gattcatcaa tgatcgacgc ccgtcccagc agatcggtat cgttcggata
    68701 ctgttcctgc aaggttttca ggaaatagtc gttgatttca aagatgatct gcaggtgacg
    68761 cggcagaatt ttacccagca tatcaaccgg ccaggtttcc agcgcctcgc tcatcagcgt
    68821 gtggttagtg taggagaaga cctgacaaca cacctcaaac gcgtcgtccc agctaaattg
    68881 gtgctcatcg atcagcagac gcatcatctc aggaatcgac agtaccggat gggtatcatt
    68941 gagatgaatc gcgattttat ccgccaggtt atcgtaggtt ttatgcaact gataatggcg
    69001 gcttaaaatg tcctgaatgg tcgaggaaac caggaagtat tcctgacgca ggcgcagctc
    69061 acgcccggag taggtggagt catccggata cagtacgcga gatacgttct cggagtggtt
    69121 tttatcttcc actgccgcga agtagtcacc ctggttgaat ttaccgaggt taatttcgct
    69181 actggcttgc gcactccaca aacgcaacgt gttggtcgcg tcggtatcgt aaccagggat
    69241 tatctgatcg taagcgactc ccagaatctc ttcggtttca atccagcgcg tttttttacc
    69301 ttcctgctga atgcgaccac caaaacggac tttatagcgc gtgttgtggc gtttgaattc
    69361 ccacgggtta ccgtattcca gccagtagtc tggcgactct ttctggctac cgttaacgat
    69421 gttctgcttg aacataccgt agtcatagcg gatgccgtaa ccgcgccccg gcaaccctaa
    69481 cgtcgccaga gaatcaagga agcaagccgc cagacgtccc aggccaccgt taccgaggcc
    69541 cggatcattt tcttcatcaa tcagctcttc gagatttaac cccatcgctt ccagtgcgcc
    69601 ctgtacatct tcgtaaattc ctagcgacaa catggcgttg gagagcgtac ggccaatcaa
    69661 aaactccatc gacaggtagt aaacctgacg agtttcttgc gacaactggg cacggtttga
    69721 acgtaaccag cgctccacga gacgatcgcg cacagcaaat aacgttgcgt tcagccattc
    69781 atgtttattg gcgacgaccg ggtcctttcc aatcgtaaac atcagcttgt aagcgataga
    69841 gtgcttaaga gcttctacgc taagcgtggg cgatgaatat gtaaacggag cattcatata
    69901 ggcgtttcct gaaaactatt tcaagcgata gtaaagctca cggtacgact tcgccgcgac
    69961 ctgccagcta aaatccattg ccatagcctg acgttgcaca aaccgccaca gtgaaggacg
    70021 ggaccacagt acaaaagcac gccgaatagc ccgtaacagc gaccaggcat tactatcttc
    70081 aaagacaaac ccactggcga cgccatctgc aaggttctca agagaacagt cagaaaccgt
    70141 atcagcaagc ccaccggtgc gccgcactaa cggcagcgta ccgtacttca atccataaag
    70201 ttgcgttaag ccgcacggtt caaaacggct gggcaccaga atgacgtccg cgccgcccat
    70261 aatgcgatgc gaaaatgctt cgtgatagcc aatctgaacg cccacctgac cggggtattc
    70321 cgctgccgcc gcaaggaaac cttcctgcag caccggatcg cccgcgccga gtagcgccag
    70381 ctgcccgccc tgctccagaa ggcccggtaa ggcttccagc accaggtcga gacctttctg
    70441 gctggtcaga cggctcacca ctgcaaaaag cggcacttta tcgtcaacct taagccccat
    70501 tgcgatttgt aactggcgct tattttccgc tttatcttcc aacgtatcgc gggtgtaacg
    70561 cgaggccaac agtaagtccg tctcaggact ccagattttc tcgtccacgc cgttcagtac
    70621 gccggaaaga cgcccttcac ggtgacgctg ttgcaacaga ccttccatac cgtaggcaaa
    70681 ctgcggttcg gtgatctcgc gagcgtaggt tggactgacc gccgtaatgt gatcggcata
    70741 gtacagaccg gccttcagga aagagatttg tccgttgaat tccagcccat gaatattaaa
    70801 gaatgaccat ggcaattgga tgtcattcat gtgatgtgca taaaacatgc cttgataggc
    70861 caagttgtgc acagtaaaca ccgacttcgc cggacgcccg cgcgccgcca gatacgcagg
    70921 cgcaaggcct gcatgccagt cgtgcgcatg caccacatca ggacgccaga atgggtcaag
    70981 cccgctggcc atttctgccc caacccaccc cagcagcgca aaacgcaata cgttgtcggt
    71041 ataggcaaat aagttggtat cgtgatacgg gcttcccgga cgatcataga gatgcggcgc
    71101 gtcaatcagg tatatgccaa ccccgttgta atgaccgaac aacagcgtga tatgtccggc
    71161 gaaggtatca cgacgggata ctacttgcgc atcggtcacg ccacggcgaa tatcgggaaa
    71221 tgcaggcaac agtacgcgag cgtcaacgcc gtctgcgatt tgtgctgcgg gtaatgcccc
    71281 aataacatca gccagaccgc cggttttaag cagcgggaac atctctgaac atacatgtaa
    71341 aacctgcatt atcgctcctg tttatgccct aacttccgta gcatttcgcg cgttaccagc
    71401 acgatgcctt cttctgaacg atagaaacga cgtgcatctt cctctgcgtt ttcaccaatc
    71461 accatgcctt ccggaataac acaagcacga tcgatgacgc agcggcgcag acggcacgag
    71521 cgacctaccc atacttccgg taacaatacg gcggaatcaa tgttgcagaa tgaattcacg
    71581 cgaacgcgcg agaacagaac ggactgcacc accaccgaac cggagatcac acagccgccg
    71641 gataccagtg agttaagggt catcccgtgg ctaccggagc gatcctgcac gaatttcgct
    71701 ggcggtaatg attcattgta ggtgcgaatt ggccaattgc gatcgtacat atccagttcc
    71761 ggcaccacag aggccagatc gaggttcgct ttccagtaag cttccagcgt acccacatcg
    71821 cgccagtacg gctcggcatc cgggtcggat tgtacgcaag agagcgggaa cgggtgcgca
    71881 taggccagac cggcttcggt gatcttggga atcaaatctt tgccaaagtc gtggctggag
    71941 ttctcatcac gatcgtcttc ttccagcagt tcatacagat agtcggcgtc aaagacgtag
    72001 atacccatac tcgccagaga tttgctcgga tcgttcggca ttgacggtgg gttagcaggt
    72061 ttttcaacga attcgataat tttatcgttc tcatcaaccg ccataacgcc aaatgcggag
    72121 gcttcttcaa tcggtactgg catacaagca acggtgcaac gtgcgccttt ttcgacgtga
    72181 tcgataagca tacgcgagta atcttgcttg tagatatggt cgcccgccag gatcaccacg
    72241 tattccgctt tataacggcg gataatatcg aggttttggg tgaccgcatc tgcggtgccg
    72301 cgataccagt tttccccttt cattctctgc tgcgctggca gcagatcgac aaactcgttc
    72361 atttcttcat taaagaatga ccagccgcgc tgaatgtgct gcaccagagt gtgggactgg
    72421 tactgggtga tcacgcccat acgacggatc ccggagttga tgcagttaga cagcgcaaag
    72481 tcgataatgc ggaacttacc gccgaagtgt acggccggtt ttgctcgctt attggttaaa
    72541 tccttcaggc gggtaccacg tcctcccgcc agtatcaggg caacagattt caatggcagc
    72601 tggcgcgcca acattaagtg atcgttcttc tctaaactaa ccatgactaa ctcctttttt
    72661 atcatctctg gaacacacac aatccgtgtg caggtccctg ccagacagcc gtaatcactg
    72721 ggttatcctc tccagcgaat gggggaatgg cgtgccactc cccagcaggt aaaacaatct
    72781 ctgttacctc aagcgtggcg ttaattgcga tcaaaaagcg atccgagagc agaatttgca
    72841 gctgtttcgg cccgttttgc cactcatccg tgcttaaagg ttgagcatat cgatttagcc
    72901 aacggacatt gccgtcgcct tcttcccacc agcgattctc caccaaagcg ggaatacgct
    72961 tgcgcagatg gattaacgcg gcggtaaatg cggttaaacc actgcttgcc tgcgaccagt
    73021 ccaaccaggt taattggtta tcctgacagt aggcattgtt attgccatgc tggctgtgac
    73081 catgttcgtc accggccagt aacatcggcg tgccctggga gagcaacaac gttgttaaca
    73141 gggcatgaat gctgtcgcgc cgccgttcaa ccagatcaag agtaccgcct aacccttctt
    73201 taccatgatt gttactgtaa ttgttgttgg tcccgtcgcg attttcttct ccgtttgctt
    73261 cattgtgttt atggttgaag caaacgcagt cgcgaagcgt aaaaccgtca tgtgcggtga
    73321 cgagattaat cgcggcactc ggcagacgac cattacgttt aaaaacatcg ctggaggcag
    73381 caaaacgccc ggcaaacgcc cccagaggca aatcataatg cagccagaaa cgacgggcag
    73441 catcgcggaa atgatcgttc cactcggcaa acagcggcgg gaaatttccc acctgataac
    73501 caccaggagc gatatcccac ggttcagcaa ttaacttcac ctgcgagagc accgggcagt
    73561 tctggatagc ggtaaacaac ggcgcatcct gacggaactc tggcgtacgg cccatgactg
    73621 ccgccagatc aaagcggaaa ccatcgacgt ggcaggtttc tacccaataa cgcaggcagg
    73681 cgctggcata atccaccacc gccggatgac tcaaattgag cgtgttgccg caaccggtcc
    73741 agttgtgata atcgccgtct tctcttatcc aataatagct acggttatcg atcccacgca
    73801 gcgagaataa cgggccgtcg aggtccagtt ccgcactatg gttgagcacg atatcaagaa
    73861 tgacttcgat acccgcttta tgcagtgctt tgattgcatc gcgaaactca tccagcgccg
    73921 tttctggcga gcaggcatac gccggatgca gcgcaaacat cgccaccggg ttgtaacccc
    73981 agtagttact tagccccatg cgttgcagac gtggttcact ggcaaactgc gccactggca
    74041 gcagttccag cgcggtaatg cccaattgtt tcaaatagtt gatcatcacc ggatgcccga
    74101 gggctttata agtgccacgg atctcgaccg ggatctccgg gtgcaagtac gttaatcctt
    74161 tgacatgggc ttcataaatg atggtgctgc cccacggcgt gcgcggcggg gcatcatctt
    74221 cccagtcata gtgatcaacc accactacgc atttcggcgc aatggcggcg ttgtcgcgat
    74281 agtcaggttc attatgaccg gcgtgcagca gcgggttatc tttaaactcc ccgtcaattt
    74341 gccgcgcgca aggatcaatc aacaacttcg ccgggttaaa gcgatgcccc tcggcgggtt
    74401 gccaggggcc atgaacgcga taaccataac gcaaacccgg gcgcgcatcc ggcagataac
    74461 cgtgccaaat gtcgccactg tgccctggca agtcatagcg atgttcctgg ccattggcgt
    74521 caaagacaca cagttctacc cgctcggcat gaacggagaa aagtgtgaag ttgacgccct
    74581 gaccgtcgta atgcgcgccg aggggagcgg gtttgccaat ggcgagttgt gtcattctgc
    74641 ctcccgaacc agccagatag tggccagcgg tggtagcgtc aggcttagtg aatgctgacg
    74701 accgtggctg gcaatctcat cgctgtgtac cgtgccgcca ttgcctgcat tactgccgtg
    74761 atagtgcatg gaatcggtat tgaggatttc acgccatttg cccggctgat taatgccgaa
    74821 gcgataatca tgacgcggta ccggcgtaaa gttactggca acgatgattt cgttaccctc
    74881 tttatcgcga cgcacaaaga tcagcaccga gcgttctttg tcatccacca ccagccattc
    74941 aaagccgtac gggtcaaaat ccagttcatg cattgcttta tggtggcggt aggtgaggtt
    75001 cagatcgcgc accagacgct ggacaccgtg gtgccagtta tcgccgcctt ccaacagatg
    75061 ccagtcgagg ctggcgtcat ggttccactc gcggccctgg gcaaattcgt tacccatgaa
    75121 cagtagtttc ttgcccggga atgcccacat ccagccatag taggcgcgca ggttcgcgaa
    75181 tttctgccat gcgtcgcccg gcatgcggtc gagaatcgat tttttaccgt ggaccacttc
    75241 atcatgcgac aacggcagga cgaagttttc agtgtagttg tagagaatcc cgaaggtcag
    75301 tttatcgtga tgatactgac gataaaccgg gtcgagcttc atgtagtcca gggtgtcatg
    75361 catccagccg aggttccact tgtaccagaa gcccagaccg cccatatcct gcggacgaga
    75421 aacgccaggg aaatcggtag actcctcagc cattgtcacc gcaccggaaa cctgctcacc
    75481 aagaatacgg ttggtattac gcaagaattc aatcgcttca agattctcgc gcccgccaaa
    75541 ttcgttcggg atccactccc cctctttacg gctgtagtcg cgataaatca ttgacgccac
    75601 cgcatcgacg cgcagcgcat caataccaaa acgttcaatc cagtaaagcg cgttaccgac
    75661 gaggaagtta ctgacttcac ggcgaccata gttgtagatc agcgtgttcc agtcctgatg
    75721 atagccttca cgcggatcgc tgtgttcata caagttcgtg ccatcaaatt cggcaagcgc
    75781 aaagtcatca gtcgggaagt ggcctggcac ccagtcgaga atcacgttca gaccagctgc
    75841 gtgtgcggca tcaatgaaat aacggaagtc gtcgcgagta ccaaaacggc gggttggtgc
    75901 atacaggccg gttggctgat aaccccaact gccatcgaag ggatgctcgt taatgggcag
    75961 tagttcgagg tgggtaaagc ccatccattt agcataaggc accagttgat cggccagctc
    76021 gcggtagctc aaccagaaat tgttgtcggt gtggcgacgc caggaaccca ggtgaacttc
    76081 ataaatagag attggcgcat caaactgatt cgcttttttg cgctcttcag tctgtacaac
    76141 cttttccggc agcccgcaaa taagagacgc ggtttccggg cgcatttgcg cttcgaaggc
    76201 ataagggtcg gacttcagac gcaagttgcc attggcatca atcatctcgt atttatagag
    76261 ctgaccgtta tgcgccccag ggataaacag ttcccagatg ccgctctctt tacgcaggcg
    76321 catcgggtga cggcgaccgt cccagtagtt gaattgccca accaccgaga cccgacgggc
    76381 gtttggagcc cagacagaga aacgcgcacc tgtgacgcca tccatagtat ctgcatgcgc
    76441 gcctaaggtt tcatacgggc gcaggtgagt accttcagat aatagccagg catccatttc
    76501 ctggattagc ggaccaaaac ggtaaggatc atcaatcagg ttttgctgac catgccagac
    76561 aacagccaac tgatagcgga aaaaattctt acgtcgcgga atgacgccgc taaagaatcc
    76621 ccgtgagtcg agacactcca gttttgcgag tttgcgcccg gttttcggtt caatcaccca
    76681 cacatcggta gcgtcgggta aaagggcacg gacttccagt cccgcggtgg ttttatgcat
    76741 tcccagtacg gaaaaaggat ccgcaaaatg gcctgcaatt agcgcgttaa tcacgtctct
    76801 atcgatacga tcggacatgc ttgtcttcct gttttattgt gtcaccccat ccaattggat
    76861 ttttgacttc tggttgtgac atttttttga cctgaacggc gcagcactct gtgcatcctc
    76921 tctgcgtcgt cctcacttca ggtaaggctg tgaatactca tgtattcagc cacccttaaa
    76981 gaatagccaa tgctctattt aactcccggt aaatcatgaa acatctgcgc ttactcctgt
    77041 attacgcact aacaggggcg gcatcgcgcc ccagatttaa tgaataaaga ttacgccagt
    77101 tgacgaagca tccgacgcag cggctccgcg gccccccaca gcagctggtc gcccactgta
    77161 aaggctgaca ggaactctgg tcccatattc agcttacgca ggcggcctac cggcgtggtc
    77221 agcgtgccgg taacggcagc tggggttagc tcacgcatag tgatttcccg atcgttcgga
    77281 acgactttcg cccacggatt gtgcgcagcc agcagttctt ccacggtcgg aatagacaca
    77341 tcttttttca atttaatagt gaatgcctgg ctgtggcagc gcaatgcccc gacacgcaca
    77401 cataaaccat ctaccggaat tacggaagat gtgttgagga tcttgttggt ttccgcctgc
    77461 cctttccact cttcgcggct ctgaccgtta tcgagctgtt tgtcgatcca cggaatcagg
    77521 ctacccgcca gcggcacgcc aaagttatcc accggcagct caccgctacg ggttaaggtt
    77581 gtgactttgc gttcgatatc gagaatagca gaggacgggg tcgcgagttc atctgccaca
    77641 tggccataca gatggcccat ctgggttaat aactcacgca tatgtcgcgc accaccgccg
    77701 gaagcggcct ggtaggttgc aacggacacc caatcaacaa gatcattggc gaataaacca
    77761 cccaacgaca tcaacatcag gcttacggta cagttaccgc caacaaaagt cctgatgcca
    77821 ttatttaatc cgtcggtaat gacgtcctga ttgacggggt caagaatgat gatggcgtca
    77881 tctttcatgc gcagagacga tgctgcgtca atccagtaac cttgccatcc gctttcacga
    77941 agctttggat agatttcgtt ggtataatcg ccgccctgac aggtcacaat gatatcgagg
    78001 gcctttagcg cctccagatc aaaggcatcc tgaagtgtgc cagtggttcc gccaaaagac
    78061 ggcgcagcct ggccaagctg agaagtagaa aagaagacag ggcgaatggc gtcgaagtcg
    78121 cgctcttcaa ccatgcgttg catgagaacg gagccgacca taccgcgcca gccgataaaa
    78181 ccaacatttt tcataagcgt tttttcctgc aaagatgtgt gctgtataaa tgtgccggtc
    78241 tcctcttggc acatctttca ccatacaaaa agcagccaaa gtcgcaagtg aaattaatca
    78301 atgatagcga agccatcagt aatgcgactt atcctgcttt gttagcacgc agaaagtccg
    78361 cggcaattat cagggaattt gagttatgaa tgaaatcatt tctgcagcag ttttattgat
    78421 cctgattatg gatccgctcg gaaacctgcc tattttcatg tccgtactga aacatactga
    78481 accgaaaaga cggcgggcaa tcatggtgcg agagttgctt attgctctcc tggtgatgct
    78541 ggtgttcctg tttgcgggtg agaaaattct ggcatttctc agcctgcgag cagaaaccgt
    78601 ctccatttct ggcggcatca ttctgtttct aatcgccatt aagatgattt tccccagcgc
    78661 ttcaggaaat agcagcgggc ttccggcagg tgaagagcca tttatcgtgc cgttggcaat
    78721 tccgttagtc gccgggccga ctattctcgc cacgctgatg ttgttgtctc atcagtaccc
    78781 gaatcagatg ggacatctgg tgattgctct gctgctggcc tggggcggca cctttgtcat
    78841 cctgctacag tcttcgctat ttttacgtct gctgggcgag aaaggggtga acgcacttga
    78901 acgcctgatg ggattgattc tggtgatgat ggcaacccag atgttcctcg acggcattcg
    78961 aatgtggatg aaggggtaac gaggagagtt tttcgaaaag aacccggtag tgtcacaact
    79021 accgggcgaa caaacttaac tcaacagctg gaacgcaatc atcccaacga tggcaccgac
    79081 agtgccgagg atggtttcca tcatggtcca ggttttcagc gtttcggctt cggtcgcgcc
    79141 ggtaaattta ccgaacaacc agaaaccggc gtcgttaacg tggctgacaa caatcgaacc
    79201 accagcgata caaatcgaca gcgccgccat ttgcgcaccg gagtagttca gttgttcaat
    79261 aaccggcatc accagtccta ccgccgttaa acaggctacg gtggcagaac cctgaatgat
    79321 gcgcactgca gcggccagca cgaagcaggt gatggctatc ggcaggccca tgccagttaa
    79381 cgcttcgccc agtgccggac ctacgccaga gtcaaccagc acctgtttga acacgccgcc
    79441 tgcaccaatc accagcagaa tgatccccgc cggttgcagc gcgtgaccgc aaatctccat
    79501 cactttgtct tttggcatgc cctgacgcat tgccaggcca taaatcgcta ccagacaagc
    79561 aaccagaatc gcggtaaacg gatgaccaat aaactcgaac cattcgtaag cggtagagcc
    79621 ttctggcaca aaacgcgcgg caatggtttt cagccccacc agcaccagcg gcaacaggat
    79681 cagcgacagg ctgaatccga aggacggcat tttgccttcg ccgagatgcg gttcgctgat
    79741 gtcgtcagga atatgcaact ccacgtaacg gctgatgaaa ttaccccaca gcggcccggc
    79801 aataatcatt cccggaattg ccgcacacag gccaatcagg atcatccagc caaaatcggc
    79861 gttcatctgc gatgccagca gcattggcgc tggccctggc accaggaacg ccgccgctgc
    79921 cgccacgcct gcgaataatg ggattaccag cttcaccagg ttcgtaccgg tgtggcgcgc
    79981 cattgagaaa gcaacgctaa tcagcagaac aatcgccact tcaaagaaca gcggtagcgc
    80041 acagaccagc cccgcaaggc cgatggcata atgcgcgcgg ctgtgaccga aggatttgag
    80101 cattttgacg gcaatctgat cgactgcgcc ggtttcatgt aagatcttgc caaacatagc
    80161 tcccagggcg acaaccaccg ccaggaagcc gagggtgcct cccatccctt tttccatcgt
    80221 cgctgcgatt ttatcgagcg gcataccgga aaaaaggcca gcccccatgg acaccaccat
    80281 taaagccagg aaagcgtgca tacgcgcctt catgactaaa aacagcagca gtaaaacaga
    80341 ccctactgct gttaaaacaa gcgttaatgt agtcactact tatttgcctt ttttaataac
    80401 ctcaatggtg cttgccacaa caccttccag cggttgatcg atatccacca ccagtacatc
    80461 ggtttcgtcc gcacccggct cctgcagcgt ttcaaactgc gtcaccaaca tttgggtttt
    80521 aaagaaatgg cctttgcgcg ctttcaggcg gctttcaatc acatcaaaat cgcctttcaa
    80581 atagatgaaa gagagattcg gattaccttc acgcagcaag tcgcgatagt gttttttcaa
    80641 tgcagaacag acgatcagcg acactttatt agtgcgctgc atagcaaacg cggcgtcgtt
    80701 cagcgcctgc aaccacggtt tgcgatcgtc gtcattcagt ggttcgccag acgccatttt
    80761 ttcgatattg cgccgtggat ggaggaaatc gccatcaaga aacgcggcat gaagttgatg
    80821 cgccacttca ctggcgaccg cagatttgcc gctgcccgat acgcccatca agacgtaaat
    80881 gtggtgatca tggttagtcg tgctcaaagt ggtgccccca caatacaaga attaaaaatg
    80941 ttacgggtaa ctgttatcgg taacattgtc cagccggaca atatcagaag caatatccat
    81001 ccatgcctta agtgtataag tgtgagctac ttcaaatttg tgggcttaaa tagatccgcc
    81061 cggtgacaag gtgaaaccta aatctaacat tttcggtgtc acagattcgc cacgaatacg
    81121 cgccagcagg cgttcagcgc caatactgcc catccgctca cgcggcgtca gcacgctcgc
    81181 aagtcgtggc tccatcacct gaccaatgtc atgaccgtgg aaaccggcaa tcgccatatc
    81241 gtcaggaact tttaacccca gacgctgaca ttcaaacgcc gcgccgaccg ccaggtcatc
    81301 attcgtacag aacacgccat ccagctgcgg atattcccgc cgcgcctggc gaatcagttc
    81361 aataccggaa gagtaagaag aagattgctc aaccatcacg ctatatggca ccaggcctgc
    81421 atccagcatc gcctgttcgt atcccttctg tttgatgata gtacgttcgt cgagacgtgc
    81481 gccgagatag gcaatgtggc gatgcccgcg agcaataatg gcagtggtca tctggcgtgc
    81541 tgcttcaaag ttatcaaaac cgacggcgat atcaaggcat ggcgacttgc tgtccatcag
    81601 ttccaccacg ggaatacccg ccacttcaat catctttaag gtgcgcggcg tgtgggtacg
    81661 ttcggtgagg agcaggccgt cgatattcca ggagagcatg gattcgaggc gttcttgctc
    81721 catttccggt ttataaccgt agtgcgccag catggtctga taaccgtgcg cgtcggtgac
    81781 gctttcgatt ccgcgtaata cttccgcgaa aacctggttg gtgagagaag gtaacaggac
    81841 gccaatcgcc cggctggtgg cgttagagag gatatcgggc gcacgattgg gaatatagcc
    81901 cagttcatca agagccgcgg caatcttgcc tcgtagagcg acggaaacct gctccgggtt
    81961 gcgtaaaaaa cggctgaccg tcattttggt cacgcctaca cggtcagcca catcctgaag
    82021 tacgggtctt ttctttttca tcgtcctgaa ggtacaaaag agatagattc tcttagttta
    82081 acacggacac atcagaacct tcccggggaa aacagtgctt gtttacaatt attagatata
    82141 cactaaatca attgcatcgc attgtgctaa cgacgcggca aaaccccgga agcgtacatc
    82201 agcacgcgac cggagcgagc gaggaagcct gcaaaaaggc aggcttcaaa gatgccgttg
    82261 agttttaaac cggcggcaga tcgaacagta acacttcgct gtcgctatcc gcatggatgg
    82321 agattgcctg ctcatcccag attgccagac catcgctggt cgaggctttc acgccgttaa
    82381 tggtgacatt gcctttcacc acctggatcc agacgcggcg ttcagcggca atctgatgca
    82441 ccgactgctc atctttcagc aacgcccagc ggtacagttc catatcctga tgcactttca
    82501 atgaaccatc tcgcgcatcc ggcgagagca ccagctgttt gccctgtacg gcatcgaagc
    82561 gacgctgttc ataacgcggc gtaataccgt tttcttcagg catgatccag atctgataca
    82621 gatgcagacg ctcggtgctg cttgggttgt actctgagtg acgaataccc gtaccagcac
    82681 tcataatctg gaactcaccc gccggaacct gctctttatt gcccatgctg tcctggtgct
    82741 caacagtacc ttccagcacg taggtcaaaa tttccatatc tttatgtggg tgagtgccga
    82801 agccctgccc tgcttcaatc acgtcgtcgt taatcacgcg cagcgcggag aagcccataa
    82861 agttcggatc gtaatagttg gcaaaagaga aagtatgcca ggagtccagc cagccatgat
    82921 ttgcatgacc acgttcattt gctttgcgta agtagatcat ttgtattcgc cccctgaatg
    82981 atttcgatgg gcttagtgtg gacccgatct gcctgggatg atagagggtg aaaattgacc
    83041 cctctgttca aaaaaattga actattcgag gggccatcaa acttacttcg cgagagtcac
    83101 tgtggaggga gaggcttgct cgaatccgcg ttcaaggatt tccagattcg taagaacttc
    83161 agattccttg acgtaatttg gcgcaccgtg ggtgatggtt tgatacaacg catcataaac
    83221 gcgcccgtaa tcgcccatct ccggcttaat ctcttctctg accgtcacgc cctcgtcatt
    83281 gacatactcc agcacaccga ccgaatcatc cgctgagaat cccggttcgc ccggcataat
    83341 attagccttc aggctggttt cctgctggtc gataccatat ttaataaacg aacctttctt
    83401 accgtgaacg ataaatttcg gataatcgat tttcaccaga tggctggttt tgacgatggc
    83461 ttttagatcg ccataaaaca gttgcgcttc gaaagtatcg tcaggattgg ctttattgcg
    83521 caggctgcgg atgtcataag cgacgtgatc cgggcgaccg aacagagaaa taatctggtc
    83581 catcgtatgc acaccaaggc catagaacgc gccatcctgc ggcaacccag gtttggtttc
    83641 tgcgaccggg cggtaatagt caaaatggct ttccacttca acaatctctc ccaacttgcc
    83701 actttcaatc gcttttttcg ctgtcaggaa gcaggagtca aagcgacgat tctgatacgg
    83761 cgtgacggtc agccctttgc ttttcgccaa cgcaaacagc tccttcgcct gggcaagcgt
    83821 cggggtgaac ggtttttcga ccagcacatt tttcccggct tccagcgcgc gtttcgcgta
    83881 ctcgaaatgg ctgtccgcgt gggtgcagac aacaaccagc ttaacatcgg gatcgtttag
    83941 tacttcgtcg agatcgctgg tgaaatggat atgggaataa atgggagcct gttcttccgg
    84001 cttcgcatgg cgacgaaaaa tatgcgcgac atgccagcta tccttgcggt taagtacata
    84061 cggcagatgg taacgggtgg tgcttttgcc gaagccaata aaggcgcagt tgatgaccat
    84121 gatttcgtcc tttttaaggt ggttatttac accttagcgc aaagcggacg tggttcctac
    84181 tggagtgcgc ataacgaaca caagcactcc cgtggataaa ttgagaacga aagatcaaaa
    84241 aaaaagccag cacccggctg gctaagtaat actggaagca atgtgagcaa tgtcgtgctt
    84301 tcaggttctc cgcgagggtc ttcctgatcg cgagacaata ataatcattc tcattcgcac
    84361 ttgtccaaca ctttttgcaa aaaaatgcat ttgactcgca ttcgaaagtc aatgatgttg
    84421 aaagggacat ttaccccaaa gaggacaaag gaatgagtga gatagtgata cgccatgctg
    84481 aaacgcgcga ttacgaggcc atcaggcaga ttcacgccca gccggaggtg tattacaaca
    84541 cgctacaagt gcctcatcct tccgattata tgtggcagga gcgactcgcc gatcgtcccg
    84601 gcatcaagca actcgtcgcc tgtattgatg gagacgtcgt gggccatctc accattgacg
    84661 tgcaacagcg cccacgccgc agtcatgttg ccgattttgg tatctgtgtc gactcccatt
    84721 ggaagaatcg cggcgtcgcc agcgccctta tgcgagagat gattgaaatg tgcgacaact
    84781 ggttgcgggt agatcgcatt gaactaaccg tgtttgtcga taacgccccg gcaattaagg
    84841 tctataaaaa atacggcttt gaaattgaag ggactggtaa gaagtacgca ttgcgtaatg
    84901 gtgaatatgt cgatgcgtat tatatggcgc gggtgaagta agatagtgcc ctttttctga
    84961 gatgggaaaa gggtgtcatt caaaatcggc ataccttcct ttaatgtatt tattttccca
    85021 ataaatatat t
//
LOCUS       DAPHBJ010000002        79869 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.002, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000002 DAPHBJ010000000
VERSION     DAPHBJ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 79869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.002"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            30..614
                     /locus_tag="RHZ76_000073"
     CDS             30..614
                     /locus_tag="RHZ76_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV2810780.1"
                     /translation="MNKGTKIAIAGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPMTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGIAATGVGIRIDDAESGNIMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            694..1389
                     /locus_tag="RHZ76_000074"
     CDS             694..1389
                     /locus_tag="RHZ76_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV2810781.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKIDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1419..3935
                     /locus_tag="RHZ76_000075"
     CDS             1419..3935
                     /locus_tag="RHZ76_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV2810782.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGVGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSVNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3946..5037
                     /locus_tag="RHZ76_000076"
     CDS             3946..5037
                     /locus_tag="RHZ76_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2810783.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSGVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKNNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5080..5940)
                     /gene="rsmI"
                     /locus_tag="RHZ76_000077"
     CDS             complement(5080..5940)
                     /gene="rsmI"
                     /locus_tag="RHZ76_000077"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDV2810784.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            6004..8040
                     /gene="lpoA"
                     /locus_tag="RHZ76_000078"
     CDS             6004..8040
                     /gene="lpoA"
                     /locus_tag="RHZ76_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HDV2810785.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDAQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITLRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            7998..8393
                     /locus_tag="RHZ76_000079"
     CDS             7998..8393
                     /locus_tag="RHZ76_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDV2810786.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTIFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8413..9003
                     /gene="diaA"
                     /locus_tag="RHZ76_000080"
     CDS             8413..9003
                     /gene="diaA"
                     /locus_tag="RHZ76_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HDV2810787.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            9013..9588
                     /gene="yraP"
                     /locus_tag="RHZ76_000081"
     CDS             9013..9588
                     /gene="yraP"
                     /locus_tag="RHZ76_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HDV2810788.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9702..10742)
                     /locus_tag="RHZ76_000082"
     CDS             complement(9702..10742)
                     /locus_tag="RHZ76_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDV2810789.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWEPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10815..11450)
                     /locus_tag="RHZ76_000083"
     CDS             complement(10815..11450)
                     /locus_tag="RHZ76_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDV2810790.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAESMRPEHEGVTILSSSELRKRAE"
     gene            11578..12096
                     /gene="yhbO"
                     /locus_tag="RHZ76_000084"
     CDS             11578..12096
                     /gene="yhbO"
                     /locus_tag="RHZ76_000084"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HDV2810791.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12076..12519)
                     /locus_tag="RHZ76_000085"
     CDS             complement(12076..12519)
                     /locus_tag="RHZ76_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HDV2810792.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            12570..12872
                     /gene="yhbQ"
                     /locus_tag="RHZ76_000086"
     CDS             12570..12872
                     /gene="yhbQ"
                     /locus_tag="RHZ76_000086"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HDV2810793.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12859..13362)
                     /locus_tag="RHZ76_000087"
     CDS             complement(12859..13362)
                     /locus_tag="RHZ76_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV2810794.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13356..13880)
                     /locus_tag="RHZ76_000088"
     CDS             complement(13356..13880)
                     /locus_tag="RHZ76_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HDV2810795.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14089..15084
                     /locus_tag="RHZ76_000089"
     CDS             14089..15084
                     /locus_tag="RHZ76_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDV2810796.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15093..15971
                     /locus_tag="RHZ76_000090"
     CDS             15093..15971
                     /locus_tag="RHZ76_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDV2810797.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16052..17059
                     /locus_tag="RHZ76_000091"
     CDS             16052..17059
                     /locus_tag="RHZ76_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HDV2810798.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17177..18421)
                     /gene="mtr"
                     /locus_tag="RHZ76_000092"
     CDS             complement(17177..18421)
                     /gene="mtr"
                     /locus_tag="RHZ76_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HDV2810799.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18575..20464)
                     /gene="deaD"
                     /locus_tag="RHZ76_000093"
     CDS             complement(18575..20464)
                     /gene="deaD"
                     /locus_tag="RHZ76_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HDV2810800.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20457..20537)
                     /gene="yrbN"
                     /locus_tag="RHZ76_000094"
     CDS             complement(20457..20537)
                     /gene="yrbN"
                     /locus_tag="RHZ76_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HDV2810801.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20644..21528)
                     /gene="nlpI"
                     /locus_tag="RHZ76_000095"
     CDS             complement(20644..21528)
                     /gene="nlpI"
                     /locus_tag="RHZ76_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HDV2810802.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21637..23772)
                     /gene="pnp"
                     /locus_tag="RHZ76_000096"
     CDS             complement(21637..23772)
                     /gene="pnp"
                     /locus_tag="RHZ76_000096"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDV2810803.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(24019..24288)
                     /gene="rpsO"
                     /locus_tag="RHZ76_000097"
     CDS             complement(24019..24288)
                     /gene="rpsO"
                     /locus_tag="RHZ76_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDV2810804.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24437..25381)
                     /gene="truB"
                     /locus_tag="RHZ76_000098"
     CDS             complement(24437..25381)
                     /gene="truB"
                     /locus_tag="RHZ76_000098"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDV2810805.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25381..25782)
                     /gene="rbfA"
                     /locus_tag="RHZ76_000099"
     CDS             complement(25381..25782)
                     /gene="rbfA"
                     /locus_tag="RHZ76_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDV2810806.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(25946..28618)
                     /gene="infB"
                     /locus_tag="RHZ76_000100"
     CDS             complement(25946..28618)
                     /gene="infB"
                     /locus_tag="RHZ76_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDV2810807.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28643..30130)
                     /gene="nusA"
                     /locus_tag="RHZ76_000101"
     CDS             complement(28643..30130)
                     /gene="nusA"
                     /locus_tag="RHZ76_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDV2810808.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30158..30610)
                     /gene="rimP"
                     /locus_tag="RHZ76_000102"
     CDS             complement(30158..30610)
                     /gene="rimP"
                     /locus_tag="RHZ76_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDV2810809.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30817..30893)
                     /locus_tag="RHZ76_000103"
     tRNA            complement(30817..30893)
                     /locus_tag="RHZ76_000103"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30857..30859),aa:Met,seq:cat)
     gene            31241..32584
                     /gene="argG"
                     /locus_tag="RHZ76_000104"
     CDS             31241..32584
                     /gene="argG"
                     /locus_tag="RHZ76_000104"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDV2810810.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLHSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(32592..34217)
                     /locus_tag="RHZ76_000105"
     CDS             complement(32592..34217)
                     /locus_tag="RHZ76_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDV2810811.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(34676..34762)
                     /locus_tag="RHZ76_000106"
     tRNA            complement(34676..34762)
                     /locus_tag="RHZ76_000106"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34726..34728),aa:Leu,seq:gag)
     gene            complement(34777..35109)
                     /gene="secG"
                     /locus_tag="RHZ76_000107"
     CDS             complement(34777..35109)
                     /gene="secG"
                     /locus_tag="RHZ76_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDV2810812.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(35337..36674)
                     /gene="glmM"
                     /locus_tag="RHZ76_000108"
     CDS             complement(35337..36674)
                     /gene="glmM"
                     /locus_tag="RHZ76_000108"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDV2810813.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(36667..37515)
                     /gene="folP"
                     /locus_tag="RHZ76_000109"
     CDS             complement(36667..37515)
                     /gene="folP"
                     /locus_tag="RHZ76_000109"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDV2810814.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(37605..39539)
                     /gene="ftsH"
                     /locus_tag="RHZ76_000110"
     CDS             complement(37605..39539)
                     /gene="ftsH"
                     /locus_tag="RHZ76_000110"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDV2810815.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(39639..40268)
                     /gene="rlmE"
                     /locus_tag="RHZ76_000111"
     CDS             complement(39639..40268)
                     /gene="rlmE"
                     /locus_tag="RHZ76_000111"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HDV2810816.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            40394..40687
                     /gene="yhbY"
                     /locus_tag="RHZ76_000112"
     CDS             40394..40687
                     /gene="yhbY"
                     /locus_tag="RHZ76_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDV2810817.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(40843..41319)
                     /gene="greA"
                     /locus_tag="RHZ76_000113"
     CDS             complement(40843..41319)
                     /gene="greA"
                     /locus_tag="RHZ76_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDV2810818.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            41567..43000
                     /gene="dacB"
                     /locus_tag="RHZ76_000114"
     CDS             41567..43000
                     /gene="dacB"
                     /locus_tag="RHZ76_000114"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDV2810819.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(43186..44358)
                     /gene="cgtA"
                     /locus_tag="RHZ76_000115"
     CDS             complement(43186..44358)
                     /gene="cgtA"
                     /locus_tag="RHZ76_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HDV2810820.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(44374..45339)
                     /locus_tag="RHZ76_000116"
     CDS             complement(44374..45339)
                     /locus_tag="RHZ76_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV2810821.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWLAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(45466..45723)
                     /gene="rpmA"
                     /locus_tag="RHZ76_000117"
     CDS             complement(45466..45723)
                     /gene="rpmA"
                     /locus_tag="RHZ76_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDV2810822.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(45744..46055)
                     /gene="rplU"
                     /locus_tag="RHZ76_000118"
     CDS             complement(45744..46055)
                     /gene="rplU"
                     /locus_tag="RHZ76_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDV2810823.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            46314..47285
                     /gene="ispB"
                     /locus_tag="RHZ76_000119"
     CDS             46314..47285
                     /gene="ispB"
                     /locus_tag="RHZ76_000119"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HDV2810824.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            47514..47792
                     /gene="sfsB"
                     /locus_tag="RHZ76_000120"
     CDS             47514..47792
                     /gene="sfsB"
                     /locus_tag="RHZ76_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HDV2810825.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(47840..49099)
                     /gene="murA"
                     /locus_tag="RHZ76_000121"
     CDS             complement(47840..49099)
                     /gene="murA"
                     /locus_tag="RHZ76_000121"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDV2810826.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(49154..49408)
                     /gene="ibaG"
                     /locus_tag="RHZ76_000122"
     CDS             complement(49154..49408)
                     /gene="ibaG"
                     /locus_tag="RHZ76_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HDV2810827.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(49568..49861)
                     /gene="mlaB"
                     /locus_tag="RHZ76_000123"
     CDS             complement(49568..49861)
                     /gene="mlaB"
                     /locus_tag="RHZ76_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HDV2810828.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(49861..50496)
                     /gene="mlaC"
                     /locus_tag="RHZ76_000124"
     CDS             complement(49861..50496)
                     /gene="mlaC"
                     /locus_tag="RHZ76_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HDV2810829.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(50515..51066)
                     /gene="mlaD"
                     /locus_tag="RHZ76_000125"
     CDS             complement(50515..51066)
                     /gene="mlaD"
                     /locus_tag="RHZ76_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HDV2810830.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(51071..51853)
                     /gene="mlaE"
                     /locus_tag="RHZ76_000126"
     CDS             complement(51071..51853)
                     /gene="mlaE"
                     /locus_tag="RHZ76_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HDV2810831.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(51861..52670)
                     /gene="mlaF"
                     /locus_tag="RHZ76_000127"
     CDS             complement(51861..52670)
                     /gene="mlaF"
                     /locus_tag="RHZ76_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HDV2810832.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            52880..53845
                     /locus_tag="RHZ76_000128"
     CDS             52880..53845
                     /locus_tag="RHZ76_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDV2810833.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEVIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            53859..54845
                     /gene="kdsD"
                     /locus_tag="RHZ76_000129"
     CDS             53859..54845
                     /gene="kdsD"
                     /locus_tag="RHZ76_000129"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HDV2810834.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            54866..55432
                     /gene="kdsC"
                     /locus_tag="RHZ76_000130"
     CDS             54866..55432
                     /gene="kdsC"
                     /locus_tag="RHZ76_000130"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HDV2810835.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            55429..56004
                     /gene="lptC"
                     /locus_tag="RHZ76_000131"
     CDS             55429..56004
                     /gene="lptC"
                     /locus_tag="RHZ76_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HDV2810836.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            55973..56530
                     /gene="lptA"
                     /locus_tag="RHZ76_000132"
     CDS             55973..56530
                     /gene="lptA"
                     /locus_tag="RHZ76_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HDV2810837.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            56537..57262
                     /gene="lptB"
                     /locus_tag="RHZ76_000133"
     CDS             56537..57262
                     /gene="lptB"
                     /locus_tag="RHZ76_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HDV2810838.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            57310..58743
                     /gene="rpoN"
                     /locus_tag="RHZ76_000134"
     CDS             57310..58743
                     /gene="rpoN"
                     /locus_tag="RHZ76_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDV2810839.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            58766..59053
                     /gene="hpf"
                     /locus_tag="RHZ76_000135"
     CDS             58766..59053
                     /gene="hpf"
                     /locus_tag="RHZ76_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HDV2810840.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            59171..59662
                     /gene="ptsN"
                     /locus_tag="RHZ76_000136"
     CDS             59171..59662
                     /gene="ptsN"
                     /locus_tag="RHZ76_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HDV2810841.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            59708..60562
                     /gene="rapZ"
                     /locus_tag="RHZ76_000137"
     CDS             59708..60562
                     /gene="rapZ"
                     /locus_tag="RHZ76_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDV2810842.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            60559..60831
                     /gene="npr"
                     /locus_tag="RHZ76_000138"
     CDS             60559..60831
                     /gene="npr"
                     /locus_tag="RHZ76_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HDV2810843.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            61045..61677
                     /gene="yrbL"
                     /locus_tag="RHZ76_000139"
     CDS             61045..61677
                     /gene="yrbL"
                     /locus_tag="RHZ76_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HDV2810844.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(61674..62402)
                     /gene="mtgA"
                     /locus_tag="RHZ76_000140"
     CDS             complement(61674..62402)
                     /gene="mtgA"
                     /locus_tag="RHZ76_000140"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HDV2810845.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(62399..63052)
                     /gene="elbB"
                     /locus_tag="RHZ76_000141"
     CDS             complement(62399..63052)
                     /gene="elbB"
                     /locus_tag="RHZ76_000141"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HDV2810846.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(63282..65618)
                     /gene="arcB"
                     /locus_tag="RHZ76_000142"
     CDS             complement(63282..65618)
                     /gene="arcB"
                     /locus_tag="RHZ76_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HDV2810847.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(65714..66643)
                     /locus_tag="RHZ76_000143"
     CDS             complement(65714..66643)
                     /locus_tag="RHZ76_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDV2810848.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            67318..71778
                     /gene="gltB"
                     /locus_tag="RHZ76_000144"
     CDS             67318..71778
                     /gene="gltB"
                     /locus_tag="RHZ76_000144"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HDV2810849.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPTDLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDALAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            71791..73209
                     /gene="gltD"
                     /locus_tag="RHZ76_000145"
     CDS             71791..73209
                     /gene="gltD"
                     /locus_tag="RHZ76_000145"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HDV2810850.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMHGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            73393..73872
                     /locus_tag="RHZ76_000146"
     CDS             73393..73872
                     /locus_tag="RHZ76_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDV2810851.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            73889..74194
                     /locus_tag="RHZ76_000147"
     CDS             73889..74194
                     /locus_tag="RHZ76_000147"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2810852.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(74270..74734)
                     /locus_tag="RHZ76_000148"
     CDS             complement(74270..74734)
                     /locus_tag="RHZ76_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDV2810853.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTINLKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(74731..75606)
                     /gene="nanK"
                     /locus_tag="RHZ76_000149"
     CDS             complement(74731..75606)
                     /gene="nanK"
                     /locus_tag="RHZ76_000149"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDV2810854.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHMLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(75603..76292)
                     /locus_tag="RHZ76_000150"
     CDS             complement(75603..76292)
                     /locus_tag="RHZ76_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDV2810855.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(76340..77830)
                     /gene="nanT"
                     /locus_tag="RHZ76_000151"
     CDS             complement(76340..77830)
                     /gene="nanT"
                     /locus_tag="RHZ76_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HDV2810856.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(77939..78832)
                     /gene="nanA"
                     /locus_tag="RHZ76_000152"
     CDS             complement(77939..78832)
                     /gene="nanA"
                     /locus_tag="RHZ76_000152"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDV2810857.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(78954..79745)
                     /gene="nanR"
                     /locus_tag="RHZ76_000153"
     CDS             complement(78954..79745)
                     /gene="nanR"
                     /locus_tag="RHZ76_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HDV2810858.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        19708 a        21559 c        19820 g        18782 t
ORIGIN      
        1 atgtacttgt tacaaggata aggttatata tgaataaagg tacaaaaata gccattgcgg
       61 gcttacttgc gcttttcgca ggtaatgccg ctgcaacaga tggtgaaata gtttttgatg
      121 gcgagatttt aaaatccgct tgtgaaatca atgactctga taagaaaatt gaagttgctc
      181 ttggtcacta taatgccgaa cagtttcgtt ctgttggcga tcgtagccca aaaattccat
      241 tcactattcc tttagttaac tgcccaatga ctgggtggga gcacgataac ggcaacgttg
      301 aagcgtcttt ccgtctatgg ctagaaacgc gggataatgg taccgttcct aacttcccta
      361 atctggcaaa agtaggctct tttgctggca tagcggcaac aggtgtgggt atccgtattg
      421 acgatgcgga aagcggaaac attatgccac tgaatgctat gggcaatgat aacacggttt
      481 atcagatccc ggcggactcc aatggtattg tcaatgttga cctcatcgct tactacgtat
      541 caaccgttga agcatcagaa atcaccccag gggaagcaga cgctgtcgtt aacgtaacgc
      601 tggattaccg ttaatacgtt acggcgttat ctgacctgtc agataacgcc cttttccttc
      661 ctctttctcg ttgtatcagg ttgaaaaata actatgtcaa aacgaacatt cgcggtgata
      721 ttaaccttgt tgtgtagctt ctgtattggc caggcgcttg caggaggaat cgttttacag
      781 cgaacgcgag tgatctatga tgccagccgc aaagaggctg cgttacctgt cgcaaacaaa
      841 ggcgcagaaa cgccttattt actgcaatca tgggtagata atatagatgg taaaagccgt
      901 gccccattta ttataacccc accgctattt cgtcttgagg ctggcgatga ctcatcactg
      961 cgaattatta aaacagctga taacctgcct gaaaataaag agtcgctgtt ctacattaat
     1021 gttcgtgcca ttccagcaaa gaaaaaatca gatgatgtta atgctaacga gttgacgctg
     1081 gtatttaaaa cacggatcaa aatgttttat cgccccgcac acctgaaggg acgggtaaac
     1141 gatgcgtgga aatcactgga atttaaacgt agtgatcatt cactcaatat atataaccca
     1201 actgaatatt acgtcgtatt tgccggattg gcagtcgata aaatcgatct cacaagcaaa
     1261 attgaatata tcgcgcccgg agaacataaa cagttaccac ttcctgcatc cggcggaaag
     1321 aacgtgaaat gggctgcgat caatgattat ggcggcagtt ccgggacaga aactcgtcca
     1381 ctgcaataac aaatataaaa aacacaggtc atcagggaat gccacaacga caccaccagg
     1441 gacataaacg cacaccgaaa cagttggcgc tcattatcaa acgctgtttg ccgatggtgc
     1501 tcactggcag cggcatgctt tgcactaccg ctaacgccga agagtattat ttcgacccca
     1561 ttatgctgga aaccacaaaa agtggtatgc aaacaaccga tctgtcacga ttttcaaaga
     1621 aatacgcaca actaccagga acttatcagg ttgatatctg gctgaataaa aagaaggttt
     1681 cacagaaaaa aattacattt accgccaatg cagagcaact tctgcagcca cagtttacgg
     1741 tagaacaact acgtgagctg ggtattaagg tggatgaaat cccggcgctg gctgaaaaag
     1801 atgacgatag cgtgatcaac tcgcttgaac aaatcattcc cggtacagct gctgaatttg
     1861 atttcaatca tcagcgactt aatttgagca ttccccaaat tgcactgtac cgtgatgcaa
     1921 gaggttacgt ctccccttct cgttgggacg atggtatacc aacgctgttt accaactact
     1981 cgtttacagg ttctgataac cgttaccgcc agggcaatcg tagccaacga cagtacctga
     2041 atatgcaaaa tggtgctaat tttggcccct ggcgattacg taactattct acgtggacac
     2101 gcaacgatca ggcgtcaagc tggaacacta tcagtagtta tttacaacgt gatatcaagg
     2161 cgttgaagtc tcagttgctt ctgggagaaa gcgccaccag cggcagtatt ttttccagct
     2221 acacctttac tggcgtgcaa ctcgcttccg acgataatat gttgccaaac agccagcgcg
     2281 gatttgcccc aacggtacgc ggtatcgcaa acagtagtgc aatcgtgact atcaggcaaa
     2341 atggttatgt gatctatcaa agcaacgtgc cagcgggtgc ctttgaaatt aacgatctct
     2401 acccctcttc caacagcggc gatttagaag tcacgattga agaaagtgac ggtacacaac
     2461 gtcgctttat ccagccttat tcttcattac ccatgatgca gcgacctggg catctaaagt
     2521 atagcgcgac cgctggacgc tatcgcgctg atgcaaacag tgatagcaag gaacccgaat
     2581 ttgctgaagc cacggcaata tatggtttga ataatacttt tacgctgtat ggcggcctgc
     2641 tcggttctga agattattat gcgctgggga tcggtatcgg cggcacactt ggcgcactgg
     2701 gcgcgttgtc gatggatatc aacagagctg acacccaatt cgataaccag cactcttttc
     2761 atggctatca atggcgtacg cagtacatca aagatatccc ggaaaccaac accaatatcg
     2821 ctgtcagcta ctatcgctat accaacgatg gctattttag ttttgatgaa gccaataccc
     2881 gtaattggaa ctataacagt cgccaaaaaa gtgaaattca attcaacatc agccagacaa
     2941 tatttgatgg ggtaagtctg tatgcctccg gttcgcagca agactattgg ggcaataacg
     3001 ataaaaacag gaatatctct gttggggttt ccggccagca atggggagtt ggttacagcc
     3061 tgaattatca atacagccgc tacactgatc aaaataatga ccgcgcactc tctttgaatc
     3121 tcagtattcc gttagaacgc tggttaccgc gtagccgggt ttcctatcag atgaccagcc
     3181 agaaagatcg cccaacccaa catgaaatgc gtcttgatgg ttcactgctg gatgatggtc
     3241 gcctgagcta tagcctggaa caaagtctgg atgacgataa caaccataac agtagcgtga
     3301 acgccagtta ccgttcacct tatggaacct tcagtgccgg atacagttac ggtaatgaca
     3361 gtagccaata caattacggc gttaccggcg gcgtggttat ccatcctcat ggtgtgacgc
     3421 tctcgcaata tctgggcaac gcttttgcgc ttattgatgc taacggggca tctggcgtga
     3481 ggatacaaaa ctatccgggg attgctactg atccctttgg ctatgcagtg gttccttatc
     3541 tcacgactta tcaggaaaac cgtctctcgg tagatactac gcagctgccc gataacgtcg
     3601 atcttgagca aacaacacag tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt
     3661 tcaacgccaa tatcggttat cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt
     3721 tgccctttgg cgctcttgcc agcaacgatg agacggggca acaaagtatc gtcgatgagg
     3781 gcggcatact atatctctct gggatatcga gtaaatcaca aagctggact gtacgctggg
     3841 gaaatcaggc agatcaacaa tgtcagtttg cttttagtac accggattca gaaccaacaa
     3901 cctctgtatt acaaggcacg gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct
     3961 cttataacag gacttttgtt gatatccaca tcctgcgctt atgcctcctc aggagggtgt
     4021 ggagccgata gcactagcgg tgcgacaaat tacagcagtg gggttgatga tgttacggtg
     4081 aaccagacag ataacgtgac aggacgggag tttacctctg caacgctaag tagcactaac
     4141 tggcaatacg cctgttcctg ctctgcgggt aaggcagtta aacttgtcta tatggtcagc
     4201 cccgtactta ccaccactgg acatcagaca ggatattaca aactcaatga cagcctggat
     4261 attaaaacca cattacaggc aaacgacatt ccaggactca caaccgacca ggttgtctct
     4321 gttaacaccc gattcacaca gataaaaaac aacacggtat attctgctgc aacccaaacg
     4381 ggtgtttgcc agggtgatac gtctcgttat ggacccgtta atattggtgc aaataccacc
     4441 tttaccctgt atgtcaccaa gccatttctc ggctcgatga ccattccgaa aacggatatt
     4501 gccgtcatta aaggcgcgtg ggtcgatgga atgggaagcc cgtctacagg tgacttccat
     4561 gatttagtca agttatcgat acagggaaat ctcaccgccc cacagtcgtg caaaattaat
     4621 cagggcgatg ttattaaggt taattttgga ttcatcaatg gtcagaagtt taccacccgc
     4681 aatgccatgc cagacggttt tactccagta gactttgata tcacttatga ctgtggtgat
     4741 acttcaaaga ttaaaaactc gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac
     4801 cagtacaacc tggtcgccag acgaagaagt tcagacaatg cgcccgatgt cggtattcgt
     4861 attgaaaatc tcggtggcgg agttgcaaat attccttttc agaacggtat ccttcccgtt
     4921 gatccttccg ggcatggcac cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt
     4981 gagctggaaa caggaaaatt tcagggcacc gccaccatta ccgtcatcgt gcggtaaaag
     5041 aagacgttgc gttttgatta acgcaacgct tcggcactct tacccctgct gctccagcgc
     5101 atacttatac agcgcatttt tcttcacgcc gtgaatttct gccgccagcg ccgccgcttt
     5161 tttcaacggc agttctgcct gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc
     5221 ttcttcctgt gctttatgac cttcgataat cagcaccatt tcgcctttgc gacggttttc
     5281 atcttctttt acccacgcca gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt
     5341 tttggtcagc tcacgcgcca gaaccacgta gcgggattcg cctaataccg caacgatatc
     5401 ttccaggcta tctaacagac ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc
     5461 ttcaatggct tttagcgcat cacggcggcc ttttgatttg gcaggtaaaa agccttcgta
     5521 acagaaacgg tcagagggta aacccgcggc gcttaacgca gtgatagcag cacacggtcc
     5581 gggtagcggc accacacgaa tccccgcttc acggcaggta cgcaccagat ggtagccagg
     5641 atcgttaatt agcggcgttc cggcatcgga aaccagcgca atgttctgcc cttcttgcag
     5701 cttcgccagc agcgtttcgg ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg
     5761 ggcattaatc ccaaaatgtt gcagcaataa accggtgtga cgagtatcct cggcggcaat
     5821 cagatcaacg gcctgtaata cctctaacgc acgctgggtg atatccgcca gattgccgat
     5881 tggcgtcggt acaatgtaaa gctggccctg agaattatcc gccgattggt gttgtttcat
     5941 tgtgtcgtcc gtattgccga tttaatattg agcattgcgt aaaaaaatat cactggatac
     6001 attatggtac cctcaacatt ttctcgtttg aaagccgcgc gttgtctgcc tgttgttctg
     6061 gcagccctga ttttcgccgg ttgtggcacc catactcccg atcagtccac tgcttatatg
     6121 cagggcacgg cgcaggctga ttctgccttt tatcttcagc agatgcagca aagctctgat
     6181 gataccagga tcaactggca attactcgcc attcgtgcac tggtgaaaga aggtaaaacc
     6241 gggcaggcgg ttgagttgtt taaccaacta ccgcaagaac tgaacgatgc tcagcgtcgc
     6301 gagaaaacac tgctggcggt agagattaaa ctggcgcaga aagattttgc tggcgcgcaa
     6361 aacttgctgg cgaaaatcac acctgccgat ttagaacaaa accagcaagc gcgttactgg
     6421 caggcaaaaa tcgatgccag ccaggggcgt ccttccattg atttactgcg cgcgttaatt
     6481 gctcaggaac cgctgcttgg cgcgaaagaa aaacagcaga atattgatgc cacctggcag
     6541 gcgctctcct ccatgactca ggaacaggcg aatacgctgg tgatcaacgc cgacgaaaat
     6601 attctgcaag gctggctgga tctgcagcgc gtctggtttg ataaccgtaa cgatcccgac
     6661 atgatgaaag ccgggatcgc cgactggcag aaacgttatc cgaacaatcc gggcgcgaaa
     6721 atgctgccaa cgcagttggt taacgtaaaa gcgtttaaac cagcctcgac caacaaaatc
     6781 gccctgctgt tgccactgaa tggccaggca gcggtatttg gtcgcactat tcagcaaggc
     6841 tttgaagcgg cgaaaaatat cggcactcag ccagtggcag ctcaggtagc tgccgcacct
     6901 gccgcagacg tagcagaaca acctcaaccg caaaccgtgg atggcgttgc cagcccggca
     6961 caagcctcgg ttagcgatct gaccggtgaa cagcctgcag cccagccggt gcctgtaagc
     7021 gccccggcga caagcaccgc agcggtaagc gcacccgcaa atccatccgc agagctgaaa
     7081 atctacgata cctcatcaca accacttagc cagatcttaa gccaggttca gcaggatggc
     7141 gcgagtattg tggtcggtcc gttgctgaaa aataacgttg aagagttgct gaagagcaac
     7201 actccgctga acgtactggc actgaaccag ccggagaata tcgaaaatcg cgtcaatatt
     7261 tgttacttcg cgctttcacc ggaagacgaa gcgcgcgatg cagcgcgtca tattcgtgac
     7321 cagggtaaac aagcgccgct ggtgctgatc ccacgcagtt cattgggcga tcgcgtagcc
     7381 aatgcgtttg cgcaagagtg gcagaaactg ggcggcggca ccgttctgca acaaaaattt
     7441 ggttccacca gcgaattacg cgcgggtgtt aacggcggtt ctggtattgc tttaacgggt
     7501 agcccgatta ctctcagagc gacaaccgac tccggcatga cgaccaacaa tccaacgctg
     7561 caaaccacgc caaccgatga ccagttcacc aataatggcg gtcgtgtcga tgcggtgtac
     7621 attgtggcaa cgccgggtga aatcgctttt atcaaaccga tgatcgccat gcgtaacggt
     7681 agccagagcg gtgcaacgct gtacgccagc tcccgcagtg cgcaagggac cgctggcccg
     7741 gatttccgac tggagatgga aggcttgcag tacagcgaaa tcccgatgct ggcaggcggt
     7801 aatctaccgt taatgcagca ggcactcagc gcggtgaata acgattattc actggctcgc
     7861 atgtatgcga tgggcgtcga tgcctggtcg ctggcaaatc atttctcaca aatgcgccag
     7921 gttcagggtt ttgaaatcaa cggtaatacc ggaagcctga cggctaaccc ggattgcgtg
     7981 attaacagga agttatcatg gctacagtac caacaaggtc aggtagtccc cgtcagttaa
     8041 ccaccaaaca gaccggcgat gcgtgggaag cacaagcgcg tcgctggctg gaaggcaaag
     8101 gactgcggtt tatcgccgct aacgtgaacg agcgtggcgg cgagattgat ctgataatgc
     8161 gtgaaggccg gaccaccatt tttgtcgagg tacgctatcg ccgctctgcg ctttatggcg
     8221 gcgcggcagc cagtgtgacc cgcagcaaac aacacaaatt attacagact gcccgcttgt
     8281 ggctcgcgcg tcataatggg agttttgata ctgtggattg ccggttcgat gtggtagcct
     8341 tcaccgggaa tgaggttgag tggattaagg atgcctttaa tgaccactca taattaaggt
     8401 ttaaggatta gcgtgcaaga aagaattaaa gcttgcttca ctgaaagcat tcaaactcaa
     8461 attgcggcgg cagaggcgct tccggatgcc atctcccgtg cagccatgac gctggttcag
     8521 tctctgctca atggcaacaa aatcctctgt tgtggtaatg gaacttccgc tgccaatgca
     8581 cagcattttg ctgccagcat gatcaaccgt ttcgaaacgg agcggcccag cttacctgcc
     8641 attgcactaa atactgataa tgttgtctta acggcgattg ccaacgatcg cttacatgat
     8701 gaagtgtatg caaaacaggt gcgggcgctg ggtcatgcgg gagatgtatt gttagccatt
     8761 tccacccgtg gcaacagccg cgatattgta aaagcggtag aagctgccgt tacgcgtgat
     8821 atgaccattg tggcattgac cggctatgac ggcggcgaac ttgcaggttt gttagggcca
     8881 caagatgtgg agatccgcat tccttcgcat cgtagtgctc gcattcagga aatgcatatg
     8941 ctgacggtaa attgcctgtg cgatctgatc gataacacgc ttttccctca ccaggatgat
     9001 taaggagaat acatgaaggc attatcgcca atcgcagtcc ttatttccgc gctgctgttg
     9061 caaggttgtg ttgccgctgc cgtagtgggt accgctgctg tgggtaccaa agccgcaact
     9121 gacccacgca gtgtcggcac ccaggtggac gatggtaccc tggaagtgcg cgtgaacagc
     9181 gcattgtcga aagacgaaca gattaagaaa gaagcgcgca ttaatgtaac ggcctatcag
     9241 ggcaaagtgc tgctggttgg gcagtcacca aatgctgaac tttcggctcg cgccaaacag
     9301 attgctatgg gcgtagacgg tgccaacgaa gtgtataacg agattcgtca gggccagccg
     9361 attggtctgg gcgaagcatc taacgatacg tggatcacca ccaaagtgcg ttcgcagctc
     9421 ttaaccagcg acctggtgaa atcgtccaac gtgaaagtga ccaccgaaaa cggtgaagtg
     9481 ttcctgatgg ggctggtgac tgaacgtgaa gcgaaagcgg cggcagatat tgccagccgg
     9541 gtgagcggcg tgaagcgggt aactacggcg tttacgttta ttaaatagca gccccttgta
     9601 atgcctgatg cgacgcttgc cgcgtcttat caggcctaca acgatacaaa ccgtaggtca
     9661 gataaggcgt ttacgccgca tccgacatgt tttccctcaa atcagaacaa cagcgccagc
     9721 ccgccgacaa tcacaccaga cactgccacc atcgcccccg tcagccataa ggctttcgcc
     9781 gggaacgctt tgcgcagcat aatcagtgac ggcaaactca ccgccgggag cgtcatcaac
     9841 aatgccagcg ccggagcggt tcccatacct gccagcatca tcgtttgtac aatcggaatt
     9901 tctgctgccg tgggaatgac aaacaagcat cctgctaccg ccatcgccac cacccacatc
     9961 aggctgttat cgacagcacc atcggcatgg gggaataacc agacgcgagc ggcacccaac
    10021 accagtactg caaggatgta aaccgggatc gtactccaga aaagcgtcca tagcgccctg
    10081 ccccagcggc taaaaaaccc gccctgtgct tccggtatgt caatttcgac cggtgcctgc
    10141 gtttgcggtg tttcacgcac ccatttttgc accagcgtcg caatcagcaa caccatcacc
    10201 agcccggcca ccagacgaat cgccgcaaaa ccccagccga ggacaaagcc cataaacacc
    10261 agcgtcgccg ggtttaacac cggattgccc atccagaatg ccagcgcacc gcccatcgac
    10321 acctgttggc gacgcattcc cgccgcgacc ggagccgcac agcaggtaca catcatgccc
    10381 ggcaacgaaa acagcgttcc cagcagcgtg ccgcgaaagc gcgattgccc aagcgtacgc
    10441 aacaaccagt cacgcgggat cagcacctga atcaacgaac cgaggatcac ccccagcacc
    10501 gccgctttcc ataccgcgag gaaatagatc atcgcgtaat ccaacgccgc ctgccatggg
    10561 ttagcatccg cctgcgcaag gatagattta ccgatactgt gggtttcggc agcagtaaac
    10621 gctttgccgt agtaaggttc ccatttcaca taccagagac cggcaatgac aacgagaaag
    10681 aaaagcgcgg gtttccacca ctgaatgggc gttgccgcct gagatgaaga ctgaccagtc
    10741 atagcattcc ccaggaataa gttatgataa ttgagcgcgt gaatattacg ctcactatca
    10801 attcttggga ataattattc agctcttttg cgtaattctg aagagcttaa aatcgtcacg
    10861 ccttcatgct caggccgcat cgactctgcc agcatgaccc gcgccacgtc tctcgcatca
    10921 atggatttcc agttacctgg taacaaacgg aacagcggcg caaaaagcgt ttcgttcatc
    10981 cgctgtttgc tacgatcgcc cagtaacatc gacgggcgag caatggtcaa tttcggccag
    11041 ttctgggcga ttaatgcttc ttccatctcc cctttgacgc ggttatagaa aaacggcgag
    11101 tgggcattgg cacccatcgc actgactacc aacatatgct gcgcgcccag tcgccgcccg
    11161 gttaatgcgg tatccactac cagcgtgtaa tcggcatgaa taaacgcctc tttgctcccc
    11221 gcttctcgcc gcgtggtgcc gagacaacaa aacacaatgt cgatgggatc ggtgacctgc
    11281 gccaacgcgt cactcagttg cggatcatgg ggattaaaca ccccgggcat atcgcccaac
    11341 ggacgtcgcg tcggcgcagc aatggcgtta actttcggtt cgttaatcaa catccgcagc
    11401 aggtgaccgc ccaccagccc cgttgcgcct gtaatcagta cctgactcat cttcgctcct
    11461 ttacagaatt gtctgccttg cgctccacgg ctcatgcacc aggcttaata gaccatgagg
    11521 taattatccc cgattgtggg aaattcgcct catccaatgc aacaacgcgg aggaagcatg
    11581 agtaagaaaa ttgccgtttt aatcactgat gaatttgagg attcagaatt tacttcaccc
    11641 gcagacgagt tccgtaaagc cggacacgaa gtgattacca ttgaaaaaca agcgggtaaa
    11701 acggtgaaag gcaaaaaagg agaagccagc gtgaccatcg ataaatccat cgatgaagtg
    11761 acgcctgcgg agtttgatgc cctgctgcta ccgggcggcc attcaccgga ttatctgcgt
    11821 ggggacaacc gttttgtcac ctttacccgt gattttgtga atagtggcaa accggtgttt
    11881 gccatctgtc acggcccgca gttgctgatc agcgccgatg tgattcgggg gcgcaaactg
    11941 accgcagtta aaccgatcat tattgatgtt aaaaatgcgg gcgcggaatt ttacgatcag
    12001 gaagtcgtgg ttgataaaga tcagctggtt accagccgga caccggacga tctgccagcg
    12061 tttaaccgcg aggcgttacg cctgctcggt gcctgagtcg cgtagccaaa tcattttttt
    12121 accaaagccc agcgtgttgt cggtgaattt gatttcatcg agccggattt cccacaccgg
    12181 ggccgacagc attctggcaa ccggaaagcg acgattgtac gccttgcgcg cgaggtcgct
    12241 ttcctcacct tccagcctgc ggatctcacc tttaaactgc acaccgcgaa ttaacgctac
    12301 cgttttcggc tgaccgttta ccgttccggc aactgccgcc tgcggcccgc tcatctgcgc
    12361 gtggcgcgtt ttttcttccg tcaaaatgta gaaggcgact ttctgcgcat caaaaagata
    12421 aaaggcatta gcgcaccaga gttccccttc ctgctgcaca caccaggtga caacatgttg
    12481 ttttgccagc caacggctga tggcgatgag tgtttccatt gctgttctct tttatactgt
    12541 gggcctgacc ttaacatgcg atgcagacga tgacaccctg gtttctttac ttgatccgta
    12601 ccgccgacaa taagctttat accgggatca ccacggatgt cgaacgccgc tatcagcagc
    12661 accaaagcgg caaaggggcg aaagcactgc gcgggaaagg agaactgacg ctggcgtttt
    12721 ctgctccagt gggcgatcgt tcgctggcgt tacgggcgga atatcgcgtt aagcaactga
    12781 ccaaacggca aaaagagcgt ctggtagcgg aaggcgcagg gtttgcagag ctgttaagca
    12841 gtctgcaaac cccggagatt aaaagcgatt gaaatgctcg tgatactcaa ccaggccggt
    12901 aacgccattc agcgcgtcat ccgctaaacg atgtacctgg aaggcgcttt cagtgcccgg
    12961 ccagcggcaa cgcagatcgt gatgcgccgc cagttcaaag ccgaaacgac tgtacagcgc
    13021 cggatcgccc agcgtcacca ctgcggcata gccgaactca ttaagcgaat cgagtccttc
    13081 atagaccagt tggcgtgcca gcccctgtcc acggtatttt tcatcaaccg ccaacggtgc
    13141 catgccgacc cattgcaggt cttcgccctg cacatcaacc ggactaaatg ccacatagcc
    13201 aatgacctga ccttcgtcat ctgtcgccac cagccccagc gtcagaaagc catcttcacg
    13261 cagatcgtga accagcttcg cttccgcatc actttcgaat gagcgacgca gcagggcatc
    13321 aatacccggc gcatcaatgg gaatttctac tcgaattagc atggttcacc taccgatgtc
    13381 tgtttggttt caggcgcggt tttcattccc gcctcaacaa aatccgccag ttgcagcagc
    13441 atcatgcgta aggctttcgg catttgctcc agttcaatgg cgtccatcag gtttttcaca
    13501 tacagcccca gctccgtatc gccttcaatc accagccgac gctggaagaa gagcgtatcc
    13561 ggatcctgtt tacgcgccgc aatcatcagc agatcgctgg cgtcggcact aaaactcaca
    13621 tcagcttgcg cgttctggct gaccaccagt ttgccattca ccaccgaggt aaaccattgc
    13681 aggtcaatat cacgcacatg aatacttaac cagcggcctt caagaaactc cagctcgcca
    13741 tcatccagcg cctgacggaa ttgccagctt aagacctgct caagaacctg gcgttttagc
    13801 gcaaatggcg tcagttttac cggtacactc aacagagatg gccccaaatg cacaatacgg
    13861 gaacgcagtt tatccaacac gagttttact ccctgtttca acaatcatcc tattttgcca
    13921 tatcagaaaa ataacatagc ggtataaatc aacaattcca tatgaaattg ctgctaccac
    13981 caatacaact ttaactgcct taaatcaaaa attgtcgcag caaggttaac taaaatccca
    14041 gttcgttaac atttttgcgt tttgatagcg caaccttcag gaaaaattat ggagctgctc
    14101 tgccctgccg gaaatctccc ggcgcttaag gcggccatcg aaaacggcgc agatgctgtt
    14161 tatatcgggc taaaagatga taccaatgcc cgtcacttcg ccggccttaa ctttaccgag
    14221 aaaaaattgc aggaagcggt gagttttgtc catcaacatc gccgcaaact tcacatcgcg
    14281 attaacactt ttgcgcatcc ggacggttac gcccgttggc agcgcgccgt ggatatggcg
    14341 gcgcagctgg gtgccgacgc gctgatcctc gccgacctcg ccatgctgga gtacgccgcc
    14401 gagcgttacc cgcatattga gcgtcatgtg tcagtgcagg cttcggcgac caatgaagag
    14461 gcgattaact tttatcatcg ccattttgac gttgcccgcg tggtgctgcc gcgcgtgttg
    14521 tcgattcatc aggtgaaaca gctggcacgg gtcacacctg taccactgga agtctttgct
    14581 ttcggcagcc tgtgcattat gtcggaaggt cgttgctatc tgtcgtcgta tctgacgggt
    14641 gagtcgccca acaccgtggg cgcgtgttct ccggcccgtt tcgtgcgctg gcagcaaacg
    14701 ccgcaggggc tggaatcccg cctgaacgaa gtgctgatcg accgttatca ggacggcgaa
    14761 aacgcaggtt atccgacgct gtgtaaaggg cgttatctgg tggacggcga gcgctatcac
    14821 gcgctggaag aaccaaccag tctcaatacc ctggaactgc tgccggagtt aatggcggcg
    14881 aatattgctt cggtgaaaat tgaaggccgc cagcgtagcc cggcgtatgt cagccaggtg
    14941 gcgaaagtct ggcgtcaggc tatcgaccgt tgtaaggccg atccgcaaaa cttcataccg
    15001 caaagcgcgt ggatggagac gctcgggtcg atgtccgaag gcacgcaaac cactcttggc
    15061 gcatatcacc gtaaatggca gtgagaaaag caatgaaata ttccttaggg ccggtgctgt
    15121 ggtattggcc aaaagagacg ctggaagatt tttatcagca ggccgccgcc agcagcgccg
    15181 acgtgattta tctcggtgaa gcggtctgta gcaaacgccg ggcaaccaaa gttggcgact
    15241 ggctggagat ggcaaaatcg ctcgccggaa gtggcaagca gattgtcctc tccacgctgg
    15301 cgctggtgca ggcatcctcg gaactgggcg aactgaaacg ctatgttgag aacggtgagt
    15361 ttctgattga agccagcgat ctcggcgtgg tgaatatgtg cgctgagcgc aaactgccgt
    15421 tcgtcgccgg gcacgcgctg aactgctaca acgcggtgac gctgaaaata ttgctcaaac
    15481 agggcatgat gcgctggtgt atgccggtgg agctttcccg cgactggctg gtgaatctgc
    15541 ttaatcagtg cgatgagttg gggattcgca accagtttga agtggaagtc ctgagctacg
    15601 gtcatctgcc gctggcctac tccgcccgct gctttaccgc gcgttcggaa gaccgtccga
    15661 aagatgagtg cgaaacctgc tgcattaagt atccgaacgg gcgcaacgtg ctgtcgcagg
    15721 aaaaccaaca agtgtttgta ctcaatggca ttcagaccat gagcggctac gtttacaacc
    15781 tcggtaacga gctggcatcc atgcagggcc tggttgatgt ggtccgcctg tcaccgcagg
    15841 gtactgacac tttcgcgatg ctcgacgcct tccgcgctaa tgaaaatggc gctgcgccac
    15901 tgccgttgac ggcaaacagc gactgtaacg gctactggcg gcggctggct gggctggaac
    15961 tgcaagcctg aacaaatagc tcactttgtt aacaacttta actactcttt aatgcagtat
    16021 taaagattaa tcggtaacaa agtgagctgt tatgactgat aaaaccattg cgttttcgct
    16081 actcgatctg gcccctatcc ccgaaggttc ttcagcgcga gaagcgttct cccactctct
    16141 cgatctcgcc cgtctggctg aaaagcgcgg ctatcatcgc tactggctgg cggaacacca
    16201 caatatgacc ggcattgcca gtgctgcaac atcggtatta attggttatc tggcagcgaa
    16261 taccaccacg ctgcatctgg ggtccggtgg cgtgatgttg cctaaccact caccattggt
    16321 cattgccgaa cagttcggca cgcttaatac actctatccg gggagaatcg atttggggct
    16381 gggccgcgcg ccgggtagcg atcagcgaac catgatggca ctgcgtcgtc atatgagtgg
    16441 cgatattgat aatttccccc gcgatgtcgc ggagctggtg gactggtttg acgcccgcga
    16501 tcccaatccg catgtgcgcc cggtaccagg ctatggcgag aaaatccccg tgtggttgct
    16561 aggctccagc ctttacagcg cgcaactggc ggcgcagctt ggtctgccgt ttgcgtttgc
    16621 ctcacacttc gcgccggata tgttgttcca ggcgctgcat ctttatcgca gcaacttcaa
    16681 accgtcggca cgactggaaa aaccatacgc gatggtgtgc atcaatatta tcgccgccga
    16741 cagcaaccgc gacgctgaat ttctgtttac ctcgatgcag caagcttttg tgaagctgcg
    16801 ccgcggcgaa accgggcaac taccgccacc gattcagaat atggatcagt tctggtcacc
    16861 gtctgagcag tatggcgtgc aacaggcgct gagtatgtcg ctggtgggcg ataaagcgaa
    16921 agtgcgtcat ggcttgcagt cgatcctgcg cgaaaccgac gccgatgaga ttatggttaa
    16981 cgggcagatt ttcgatcacc aggcgcgcct gcattcgttt gaactggcga tggacgtgaa
    17041 ggaagagttg ttgggatagt gtgtcttaac gcgggaagcc ttatccgagc tggcaacgct
    17101 gtcctacgta gacctgataa gcgaagcgca tcaggcattg tgtaggcagc agaaatgtcg
    17161 gataaggcac cgctgattac tgatacaccg gcagcaaatt aaagctcgat aagatatgga
    17221 ccagtgcgtt gccaacgcca aacaccagaa tcagcgtaat catcggcttg ccgccccaga
    17281 cacggaattt cgggctgcca aagcgtttac gcgatgcacg ggctaacagc gccggaacaa
    17341 ttgccgccca gatggtagcc gctaaaccag cataaccaat ggcgtacagg aatccgttcg
    17401 ggaacagcag cccccccaca actggcgggg caaaggtcag caatgccgtt ttcaagcggc
    17461 ccacagctga gtcatcgaaa ccaaacagat ctgccagata gtcaaacaaa cccagcgtta
    17521 cgccaaggaa cgaactcgct accgcaaagt ttgagaacac gaccagcaga aggtccaggc
    17581 tacggctgtt cagcacgcca cttaatgcct gtaccagcac atcaatgtta ccgcccttct
    17641 cggcgatgcc aataaactcc ggacgaggaa tgttgcccat cgtcgccagc aaccagatgg
    17701 tatacagcgc cagtgccatc agcgtaccgt acaccaggca tttcacgatg gttttcggat
    17761 ctttgccgta atacttcatc aggcttggca cgttaccgtg ataaccaaac gacgccaggc
    17821 agaacggcaa tgtcatcagc agatacggcg cataagatgc attgctttct gcaacgttga
    17881 acaatgtcgc aggctgcaca tgccccaaca ggctgccaaa ggtgaggaag aaggtaatga
    17941 ctttcgcccc cagcacaatc gccgtcatgc ggctgacggc cttagtactc aaccacacca
    18001 caaacgctac cagcagtgca aaaccaaaac ccgccgcccg tgccgggacg tttagcgaca
    18061 tctctgcgaa ggtgtgatgc agaattgaac cactggcaga aatataggca taggtcagga
    18121 tatagagcac aaaggcaatg gaaataccgt tgaccacgtt ccagcctttg cccagcaaat
    18181 ctttggtgat ggtgtcaaaa ctcgaaccga ttcggtaatt caggttagct tccagaatca
    18241 tcaagccgga atgcagcata cagaaccagg taaagatcag cgccgccatt gaccagaaaa
    18301 accacgcccc ggacatgacc actggcagag aaaacatccc tgcgccaata atggtgccgc
    18361 cgataatcac cacgccgcca agcagcgacg gtgacgtttg ggtggtggtt agtgttgcca
    18421 tgagggcttc tctccagtga aaaatagtgc gactacgttg ttatgtattg cactgtacca
    18481 gtacacgagt acaaaagaca gaaaaaaagc cccgatggtt aaaatcgggg ctgtatatat
    18541 tattttacag attgttttcg ctgttcagcg atgattacgc atcgccaccg aaacgacgac
    18601 gaccggtaga gtcatcacga cgcggtgcgc ggccttcacg acgttcgccg ctaaaacgac
    18661 gaccatcacc acgaccacct tcacggcgtt caccgctgaa gttacgaccg ccttcacgac
    18721 gttcgccacc gaaaccacga ccaccgccac gacgctcacc gccagtatgc ggctgtgcat
    18781 cgcccagtaa ctgcatgttc atcggcttgt tgagaatgcg agtgcgcgta aagtgttgca
    18841 gcacttcacc cggcatacct ttcggcagtt cgatggtgga gtgagaagca aacagcttga
    18901 tgttaccaat gtaacggctg ctgatgtcgc cttcgttagc aatcgcacca acgatatgac
    18961 gaacttcaac accatcatcg cggcccactt caatgcggta cagctgcata tcgccaacat
    19021 cacgacgttc acgacgcgga cgatcttcac ggtcaccacg cgggccacgg tcgttacgat
    19081 cgcgcggacc acggtcatca cggtcacgga attcacgttt cggacgcatc ggcgcatctg
    19141 gcggtacgat cagagtacgt tcaccctgtg ccattttcag cagtgcggcg gccagagttt
    19201 cgaggtccag ctcttcacct tctgcagtcg gctgaatttt gctcagcagt gcgcggtatt
    19261 gatccagatc gctgctttcc agctgctgct gtactttagc ggcgaatttt tccagacggc
    19321 gtttgcccag cagttctgcg ttcggcagtt ctacttccgg aatagtcagc ttcatagtac
    19381 gttcaatgtt gcgcagcaga cgacgctcgc ggttctcaac gaacaacagc gcgcggccag
    19441 cacgacccgc acgaccggta cgaccgatac ggtgaacgta agactcagaa tccataggga
    19501 tatcgtagtt aaccaccagg ctgatacgct caacgtccag gccacgggcc gcaacgtcgg
    19561 tcgcaatcag gatgtccaga cgaccgtctt taaggcgttc cagtgtctgc tcacgcagcg
    19621 cctggttcat gtcaccgttc agcgcggcgc tattgtagcc gttacgctca agagcttcag
    19681 ccacttccag agtcgcgttt ttggtacgaa cgaagataat cgccgcatca aaatcttccg
    19741 cttccaggaa acgtaccagt gcttcgtttt tacgcatacc ccacacagtc cagtagctct
    19801 ggctgatgtc aggacgggta gtcacgctgg actgaatacg cacttcctgc ggctctttca
    19861 taaagcggcg ggtaatgcga cgaatcgctt ccggcatggt tgcagagaac agagcggtct
    19921 gatgaccttc cgggatctgc gccataatgg tttcaacgtc ttcgatgaag cccatgcgca
    19981 gcatttcgtc agcttcatcc agaaccagac cgctcagttt agagaggtcc agagtgccac
    20041 gtttcaggtg gtccagcaga cggcccggag taccgacaac gatctgcggc ccctgacgca
    20101 gggcgcgtaa ttgcacgtca taacgctggc cgccgtacag ggcaaccaca tttacgccgc
    20161 gcatgtgttt agagaaatcc gtcattgctt cagcaacctg taccgccagt tcgcgggtcg
    20221 gtgccagcac cagaatctgt ggtgctttca gctcaggatc aagattctgc agcagaggta
    20281 aagagaatgc tgcagttttt ccgctccccg tctgggccat acccagaacg tcgcggccat
    20341 tcagcagatg tggaatacac tctgcctgaa ttggagatgg tttttcgtaa cccagatcgt
    20401 taagggcttc aaggatagga gccttcaggc ccagatctgc aaaagtggtt tcgaattcag
    20461 ccatgtagta cgtgtgcctc aaaattaatg gcggccagtc tacataactc atcatgaaat
    20521 tgatcagcaa ttttcattga aaagtgtgaa ccggctcaaa gtaggtgtat taacgaacaa
    20581 caacgccctc acccgttaag gtgatggcaa tcaaaaaaga ttacgggctg atgtgtacgt
    20641 cagctattgc tggtccgatt ctgccaggtc atcttggtcc tggcccagga gcgataattc
    20701 caacaatgcg tatcggtgct caacaaagtt atgaacgttg ttggcaaccg ccagtttgaa
    20761 cagtgccgtg gcgctgtcca aatcccccag acttaggtag tacttaccta aatagaagtt
    20821 ggtttcactg agatgctcag cgagcgaggt gttatccgtt gcgtccgcct tgagcctttc
    20881 cattaacgtt tgttcgctaa tgttgcccag gtagaactcg acaatgttcc atccccactg
    20941 ttccttatcc gatttttcga agtgctgttt caacacttct ttagcctgct tctcatcgag
    21001 cttctgctcg gcgagataaa gccacagact acggaaagga tcattgggat cgtcttgata
    21061 aaacgccagc agatcatctt gcgctaactt gtcacgaccg ccgtaatata atgcgatccc
    21121 gcgattcaag tgcgcgtagt tgtaagttgg atcaagctca agtacagaat caaacgcttc
    21181 ataggcagca tcaaaattgc ctgcctgcgt taaatatatg cctaagtaat tgaatacttc
    21241 aggcatatcc ggtcggattg ccagcgcttg cgaaaaatcg ttacgcgcta atgccctcag
    21301 accgagacta tcatacaaca ctccgcgctc atataaaagc tgtgcgcgtt cgtcatcggt
    21361 taaagcccga ctggcaagga tttgttccat acgtgccaga atcacttcct gctgtaaagt
    21421 cggttgcaat ggtaccgcga ggacttcact tttacgccag gaagtattac tgcatcctgc
    21481 aagcgtaagt gctgtcgcaa cgaaacacca gcgcaaaaaa ggcttcattt cccactcccg
    21541 aagaccacgg ttgaatgaac gtcctgttcc cggttgctaa caaggcgtcc tgcccggtta
    21601 aaagcccccc gccgcagcgg agggcaaatg gcaaccttac tcgccctgtt cagcagccgg
    21661 agcttccggt gctgcagcag gttgagactg ctcagtcgct tctttaatgc tcagacggat
    21721 acggccctgg cgatcaactt ccagaacttt caccggtact tcctgaccca tctgcaggta
    21781 atcggtcact ttctcaacgc gtttgtcagc gatttgagag atgtgtacca gaccttcttt
    21841 accgccgccg atggcaacaa atgcgccaaa gtcaacgata cgggtcactt taccagtgta
    21901 gacgcggccc acttcgattt ctgcagtgat ctcttcgata cgacgaatag catgtttcgc
    21961 tttctcgccg tcggtcgctg caatcttcac agtaccgtca tcttcgattt cgatggtggt
    22021 gccagtttct tcggtcagag cacggattac agaaccgcct ttaccgataa catctttgat
    22081 cttgtccggg ttgatcttga tggtatggat acgcggtgcg aactcagaga tatcgccacg
    22141 cggcgcgttg atcgcctgtt ccattacgcc caggatatgc agacgcgcac ctttagcctg
    22201 gttcagcgca acctgcatga tctctttggt gataccttca attttgatat ccatctgcaa
    22261 tgcagagata ccgtcgcggg aacccgcaac tttgaagtcc atatcgccca ggtgatcttc
    22321 gtcgcccaaa atgtcagaca gtacaacgta gttgtcgcct tctttcacca gacccattgc
    22381 gatacccgca acggcagctt tgatcggcac acctgcgtcc atcagcgcaa gagacgcgcc
    22441 gcacacggaa gccatagaag aggaaccgtt ggattcagtg atttcagaca caacacgtac
    22501 ggtgtacggg aatttgtcca tatccggcat gactgccagc acgccgcgct tcgccagacg
    22561 accgtgacca atttcacgac gcttcggaga accgaccatg ccggtttcgc ctacggagta
    22621 cggagggaag ttgtagtgga acaggaaggt atcggtacgt tcgcccatca gttcatcaag
    22681 aacctgcgcg tcacgagcag tacccagcgt tgcggtaacc agcgcctgcg tttcaccacg
    22741 ggtgaacagc gcagaaccgt gagtacgcgg cagcacgcca gtacgcacat ccagaccacg
    22801 gatcatatct ttttcacgac cgtcgatacg cggttcgcct gccagtacgc ggctacgaac
    22861 aacgtttttc tcgatagcgt gcagaatttc acccagttcg ttttcgtcca gggtttcgtc
    22921 ttcagcaagc agcgtcgcga tggtttcaga tttgatgaca tcaacctgcg cataacgctc
    22981 ttgtttgtcg gtgatgcggt aagcatcgct caggcgagct tcagccagtg cagcaacgcg
    23041 cgcgttcagc gcttcgttta ccggctccgg ctgccagtcc caacgtggtt taccggcttc
    23101 tttcaccagt tcattgatgt tctgaataac aacctgctgt tgttcatgac cgaacactac
    23161 tgcgcccagc atctggtctt cgctcagcag ttcagcttca gattcaacca tcagaacagc
    23221 ggcttcagta ccggcaacaa ccagatccag tttgctctct ttcagctcgt cctgagtcgg
    23281 gttcagtacg tactggtcat tgatgtaacc tacgcgagca gcaccaatcg gaccattgaa
    23341 cggaatacca gacagagaca gtgctgcgga agcaccaatc atcgcgacga tatccgggtt
    23401 aacttgcggg ttaacagaaa ccacggtggc gataacctga acttcgttga cgaagccttc
    23461 cgggaacagc gggcgaatcg ggcggtcaat cagacgcgcg atcagggttt cgccttcgct
    23521 tgggcggcct tcacgacgga agaagctacc cgggatacga ccagcagcgt aggtacgctc
    23581 ctgatagtta acggtcagtg ggaagaagtc ctgacctggt ttggcttttt tctggccaac
    23641 aacggtaacg aataccgcgg tgtcatccat gctaaccata acagcggcag tagcctgacg
    23701 agccatcatg ccggtttcca gagtcacggt gtgttggccg tactggaatt tacgaacgat
    23761 cggattaagc aatgtaatat cctttctctt tcttagacag taccttacgg cactggtgtt
    23821 aatacccgat cttctgcgca tcctcgcgac taatgacaac cctaacccag ctctatgtgg
    23881 gtaaagcctc tcattagccg cgcgaacctc tgcaacggaa gatcattcat agcaacaata
    23941 cattagtttc cagtgaattg ctgccgtcag cttgaaaaaa ggggccactc aggccccctt
    24001 ttctgaaact cgcaagaatt agcgacgcag acccaggcgc tcgatgagct gggtgtaacg
    24061 tgctacgtct ttacgtttca ggtagtcgag cagtttacga cgctgagaaa ccatgcgcag
    24121 cagaccacga cggctgtggt gatctttttt gtgctctgca aagtggccct gcaggtggtt
    24181 gatctgtgca gtcagcagtg ctacctgaac ttcggtagaa ccggtgtcgt ttgcgtcacg
    24241 accaaactca gaaacgattt tagctgttgc ttcagtactt agagacattt taaaactcca
    24301 aagtatatag aatgaaagga cgccgatctc taattcagcg atcccagtgt acgttacgca
    24361 aagtgttaaa caatttacgc gacgttaagc ggcagtattc tactcgtagc gacctgttat
    24421 cgcaagacgg ttaacattac gccgggtatt caaccaccag gcgacgaggc gcaacgcggc
    24481 cttcatcgtc aatttcgccc ataccgataa atttgccgtt ctcaccttcc gtgacgcgaa
    24541 ccagtccttc cagtggcgca ccagatgtac gaaccgggtt accatttttg aagtaaacag
    24601 aagacgttaa cggaagattc accaccggat agtccgaagc tggactgtcc attggcatca
    24661 gtaatggatc aagtaactcc gcggctggaa tatcctgctg ttcagcttgc tcaacaagtt
    24721 cacgcaggtg ctccagggtc accatccgtt caaccggata tttacttacc gccagacggc
    24781 gcaggtaaat aacatgcgcg ccacagccga gtttttcacc caggtcatca atgatggtgc
    24841 ggatataagt gccttttgag cagtgaattt ccagctccag ctcattgcct tcatggcgaa
    24901 taaacagcaa ttcataaacg gtaatcggac gcgcttcacg cggaacttca atgccctgac
    24961 gcgcatattc gtacagtttt ttgccctgat atttcagtgc tgaatacatc gaagggatct
    25021 gttcgatatc gccacggaaa gtatccagtg ccgctgccag ctgctctgcg ctaaaggtta
    25081 ccggacgttc ttcaacgatc tgtccgtcgg catcagaagt atcggtacgc tgtccaagac
    25141 gcgcaatgac ccgatagcgt ttgtcggagt ccagcagata ctgggaaaac ttcgtcgctt
    25201 ccccgaggca aatcggcaac atgccggtcg ccagcgggtc cagcgcaccg gtatgcccgg
    25261 cacggttggc gttatatata cgtttcactt tttgcagcgc atcgttgctg gacataccct
    25321 gaggtttatc cagcaacaaa acgccgttaa tgtcgcgacc gcgacgacga ggacgactca
    25381 ttagtcctcc ttgctgtcgt ccgggttaac acgacgttct tcgtcatgtt tgaccacgct
    25441 ggtcaccagg tttgacatgc gcatcccttc aaccagagag ttgtcgtaga agaaggtcag
    25501 ttccggcacg atacgcaggc gcatcgcttt ccccagcagg ctgcggatga aaccagaagc
    25561 ttcttgcaac gctttgatgc ccgctttaac cgcgtcttca tctttgtcgt tgaggaacgt
    25621 cacatatact ttggcatacg ccaggtcgcg agacatttcg acaccggaaa cggtggtcat
    25681 catgcccagg cgaggatctt taatttcacg ctgcaggatg agagcgatct ctttttgcat
    25741 ttcctgcgct acgcgctgcg ggcgaccaaa ttctttcgcc ataataaatt ctcctgacaa
    25801 aaaaggggct gttagcccct ttttaaaatt aatttcaggt ggaagggctg ttcacgttga
    25861 cctgataaga cgcgccagcg tcacatcagg caatccatgc cggatgcagc gtaaacgcct
    25921 tatcccgcat ggaaccctaa aaaccttaag caatggtacg ttggatctcg atgatttcga
    25981 atacttcgat cacatcgcca gtgcggacgt cgttgtagtt cttaacgccg ataccacatt
    26041 ccataccgtt acggacttcg ttaacgtcat ctttgaagcg gcgcagggac tccagctcgc
    26101 cttcgtagat aaccacgttg tcacgcagaa cgcggatcgg gttgtgacgt ttaaccacac
    26161 cttcggtaac catacagcct gcgatggcac caaatttcgg cgatttgaac acgtcacgaa
    26221 cttccgccag accgataatc tgctgtttca gttccggaga cagcataccg ctcatcgccg
    26281 ctttcacttc gtcaatcagg ttatagatga cggagtagta acgcagatcc aggctttccg
    26341 cttcaatcac tttacgtgca gaggcatcag cacgtacgtt aaagccaacc aggatggcgt
    26401 tggacgccgc agccagggtg gcgtcggttt cggtgatacc acctacgcca gaaccgatga
    26461 tcttcacttt aacttcgtca gtagacagtt tcagcaagga gtcggagatc gcttcgacag
    26521 aaccctgtac gtctgccttc aggacgatat tcacttcgtg aacttcgcct tcggtcatgt
    26581 tggcgaacat gttctcgagt ttagatttct gctgacgcgc cagtttaact tcgcggaatt
    26641 taccctgacg atagagtgca acttcacgcg ctttcttctc gtcacgtaca acggtaactt
    26701 catcacccgc agccggtacg ccggacaggc cgaggatttc caccggaatg gacggacccg
    26761 cttccagcac ttcctgaccc agttcgttac gcatcgcacg aacacgaccg tattcgaagc
    26821 cacacagaac gatatcgccc ttgtgcagag taccttcacg taccagaacg gtagcaaccg
    26881 gaccacgacc tttatcgagg aaggattcga taaccgcacc gctcgccata cctttacgta
    26941 ccgctttcag ctccagaact tccgcctgca gcaggatagc gtccagcagt tcatcgatac
    27001 cggtacccgc tttcgcagat acgtgtacga actggctttc accgccccac tcttccggca
    27061 ggatgccgta ctgggagagt tcgttcttaa cgcgatccgg atcagcttct ggtttatcga
    27121 tcttgttcac tgcaaccacc accggtacct gcgccgcttt cgcgtgctgg attgcttcga
    27181 tggtctgcgg catcacaccg tcgtcggcag caacaaccag gactacgatg tccgttgcct
    27241 gcgcaccacg agcacgcatt gaagtaaacg cggcgtgccc cggggtgtcc aggaaggtga
    27301 tcatgccgtt ttcagtttca acgtggtatg caccaatgtg ctgggtaatg ccgcccgctt
    27361 cgccagaggc cactttcgtt gaacgaatgt agtccagcag agaggtttta ccgtggtcaa
    27421 cgtgacccat gatggtcaca accggcgcgc gcggttcagc cgcagcaccc gtgtcacggt
    27481 cgctcattac cgcctcttcc agctcgtttt cacgacgcag gataacttta tggcccatct
    27541 cttcagcaac cagctgtgcg gtttcctgat cgataacctg gttgatggtt gccattgcgc
    27601 ccagtttcat catcgctttg atgacctgag agcctttaac cgccatcttg ttcgccagtt
    27661 cgccaacggt gatagtttcg ccgatcacaa cgtcacggtt aacggcctga gcaggcttct
    27721 ggaagccttg ctgcagcgaa gaacctttac gttttccgcc tttaccgcca cgtactgctg
    27781 cgcgtgcttc ttcacgatca gcttttgatt cagcgtgttt gttgcctttc ttcggacgcg
    27841 ctgctttcgc gttacgacca cggccacggc cgccttcgac ttcacgatcg ctttcgtctt
    27901 ctgcctggcg agcatgttga gaagtagtga cgtgataatc gctggaatct tcagtcggtt
    27961 ccgcgttatc agtccatttg ttttcttccg ccatacgacg tgcttcttca gcaacgcgac
    28021 gtgcttcttc ttcgagttta cgacgcgctt cttcttcagc tttacgcttg agctctgcag
    28081 cttcctgctc acggcgggct ttttcagcct gggcgttttt agtcatatcg tcttgttgat
    28141 tgctcacttt gtctttttcc gcagcttcac gtttcgcttg ttcagcagct tcacgcttag
    28201 cttgttctgc ggcctcacgt tcagcttttt gttgcgcctc gcgtttagcc gattcttctg
    28261 cctcacgacg ggcttgctct tccgcttcac gctgcgcttg ctcttccgct gcaaggcgtt
    28321 cagcctcttg cggatcgcgt ttcacaaagg tgcgtttctt gcggacttcg atttgtaccg
    28381 atttgctttt tccaccggta ccaggaatgt taagggtgct gcgtgtttta cgttgcagcg
    28441 tcaatttgtc cgggcctgaa tttttctgat tcaggtggtc aatcaaagtc tgtttctctt
    28501 gtgcagacac agagtcgtca gcagacttcc ggatacctgc atcagcaaat tgctgtacca
    28561 ggcgttccac ggaggtctgt cgctctgcgg ccagcgtttt aatcgttaca tctgtcatgc
    28621 tgttccttcc tgctacagtt tattacgctt cgtcaccgaa ccagcaaata ttacgggcag
    28681 ccataatcag tgctccggct ttttcgtcgg tcaacccttc gatatcagcc agatcatcaa
    28741 tgccctgttc ggcgagatct tccagcgtac aaacgccacg ggcggccagt ttgaatgcca
    28801 aatcacgatc taccccttca aggttcagca gatcgtcagc cggtttgtta tcaccgaggc
    28861 tttcttcctg ggcctgtgca atggtggcca gtgcattttt agcacgctcg cgcagtgctt
    28921 caacggtcgg ctcatcaagg ccttcgattt ccaacagctc tttcatcggc acataggcca
    28981 gttcttccag cgtcgagaag ccttcttcta ccagaacagt cgcgaagtct tcgtcgatgt
    29041 cgagatattt ggtgaaggtg tcgatcgctg cgtgcgcttc cgcctgatgc ttagcctgca
    29101 ggtcgtcaac ggtcatcacg ttgagttccc aaccgctcag ctgcgaagcc agacgcacgt
    29161 tctgaccgtt acggccaatc gcctgcgcca ggttaccggc ttcaacggcg atatccatgg
    29221 tgtgtttatc ttcatccacc acgatagaag caacgtctgc cggtgccatt gcgttaatca
    29281 cgaactgcgc cgggttatca tcccacagga cgatatcgat acgctcgcca cccagttcag
    29341 tagacaccgc ctgaacacgc gcgccacgca tacctacgca agcacctacc ggatcgatac
    29401 gtttatcgtt ggttttcacc gcgattttcg cacgagaacc cggatcgcga gccgctgctt
    29461 taatttcaat cacttcttcg ccgatttctg gcacttcaat acggaacagt tcgatcagca
    29521 tttccggctt ggaacgagtg acgaacagtt gcgcgccacg cgcttccggg cgaacggaat
    29581 aaagcacgcc acgaacgcgg tcgccagggc ggaagttttc acgcggcagc atatcttcac
    29641 gcaggatcac ggcttcagcg ttgttgccca gatccagaga gatgttgtcg cggtttactt
    29701 ttttcaccac gccggtgatg atttcacctt cgtgttcacg gaactgatca accaccatcg
    29761 cacgttcggc ttcacgcact ttctgcacga taacctgttt tgccgtctgg gtagtgatac
    29821 ggtcaaaggt aacagactca atctgatctt caacgtaatc gcccaggttc aggctttcat
    29881 cttcataacg tgcggcttca agggtgattt ccttggtcgg ctgggtgact tcatcaacaa
    29941 ctaaccagcg acggaaggtg tcaaaatcac cgcttttgcg atcgatctgt acgcggacgt
    30001 cgatctcttg ttcatatttt ttctttgttg ctgtcgccag cgcgctttcc aatgcttcga
    30061 aaatcttctc gcgaggtagc gccttttcat tggatacggc ttcaactaca gccaaaattt
    30121 ctttgttcat cgcgggcttt tcacctcatc cagactatta aaagtgggga accaggttcg
    30181 ccttctggat attactcagc gcgaacactt catctttacc ttcgacggta actgtgatca
    30241 tttcaccgtc taccgctttg ataacgccct gccatttacg acggttttgt accgccatac
    30301 ggagaaccag agtcacctct tctccgacaa aacgggcgta gtgttcagcc gtgaacagtg
    30361 ggcgatcgag acccggtgag gagacttcca ggttataagc aacggtgatg ggatcttcaa
    30421 catccagcac agcacttacc tggtggctca catcagcaca atcatcaaca ttgatgccat
    30481 cttcactatc aatatagatg cgcagtgtgg atgtgcgacc gcgaataaat tcgatgccaa
    30541 ccagttcaaa acccagggcc tcaactggcg cagtaatcat ctctgttaat ttttgctcta
    30601 atgtggacaa gcccaccccc aagacataaa aaaagggcct aaagcccagt tattctgtag
    30661 tcagataaca aaaaaccccg ataaatcggg gctttatata actgaaccct ataaccgcaa
    30721 ctgcggtctg gagcactttc cagaaggatt ttttcaaatc ccactacgaa ggccgaagtc
    30781 ttcacagtat atttgaaaaa gaactctaag ggaaagtggt tgcgggggcc ggatttgaac
    30841 cgacgacctt cgggttatga gcccgacgag ctaccaggct gctccacccc gcgcctgaaa
    30901 cgtggcaaat tctactcgtt ttgggtaaaa aatgcaaata ctgctgggat ttggtgtacc
    30961 gagacgggac gtaaaatctg caggcattat agtgatccac gccacatttt gtcaacgttt
    31021 attgctaatc atgtgaatga atatccagtt cactttcatt tgttgaatac ttttgccttc
    31081 tcctgctctc ccttaagcgc attattttac aaaaaacaca ctaaactctt cctgtctccg
    31141 ataaaagatg attaaatgaa aactcattta ttttgcataa aaattcagtg agagcggaaa
    31201 tccaggctca tcatcagtta attaagcagg gtgttatttt atgacgacga ttctcaagca
    31261 tctcccggta ggtcaacgta ttggtatcgc tttttctggc ggtctggaca ccagtgccgc
    31321 actgctgtgg atgcgacaaa agggagcggt tccttatgca tatactgcaa acctgggcca
    31381 gccagacgaa gaggattatg atgcgatccc tcgtcgtgcc atggaatacg gcgcggagaa
    31441 cgcacgtctg atcgactgcc gcaaacaact ggtggccgaa ggtattgccg ctattcagtg
    31501 tggcgcattt cataacacca ccggcggcct gacctatttc aacacgacgc cgctgggccg
    31561 cgccgtgact ggtaccatgc tggttgctgc gatgaaagaa gatggcgtga atatctgggg
    31621 tgacggtagc acctataaag gaaacgatat cgaacgtttc tatcgttatg gtctgctgac
    31681 caatgctgaa ctgcagattt acaaaccgtg gcttgatact gactttattg atgaactggg
    31741 cggccgtcat gagatgtctg aatttatgat tgcctgcggt ttcgactaca aaatgtctgt
    31801 cgaaaaagcc tactccacag actccaacat gcttggtgca acgcatgaag cgaaggatct
    31861 ggaatacctc cactccagcg tcaaaatcgt caacccgatt atgggcgtga aattctggga
    31921 tgagagcgtg aagatcccgg cagaagaagt cacagtacgc tttgagcaag gtcatccggt
    31981 ggcgctgaac ggtaaaacct ttagcgacga cgtagaaatg atgctggaag ctaaccgcat
    32041 cggcggtcgt cacggcctgg gcatgagcga ccagattgaa aaccgtatca tagaagcgaa
    32101 aagccgtggt atttacgaag ctccggggat ggcactgctg cacattgcgt atgaacgcct
    32161 gttgaccggt attcacaacg aagacaccat tgagcagtat cacgcgcatg gtcgtcagtt
    32221 gggccgtctg ctgtaccagg ggcgttggtt tgactcccag gcgctgatgc tgcgtgactc
    32281 tctgcaacgc tgggttgcca gccagatcac tggtgaagtt accctggagc tgcgccgtgg
    32341 gaacgattat tcaatcctga ataccgtctc agagaacctg acctacaagc cagagcgtct
    32401 gacgatggaa aaaggcgact cggtgttctc gccagatgat cgtattggtc aattgaccat
    32461 gcgtaacctg gatatcactg atacccgcga gaaacttttc ggttatgcca aaactggcct
    32521 gctttcctcc tctgccgctt caggcgtgcc gcaggtggag aatctggaaa acaaaggcca
    32581 gtaattcgac atcaaccctg cccttcaggg gtggggttac cttgcttatc ctctgtaaac
    32641 tcctttctga gcatcacata atcgaaaaca tcatggtttg catccacaac ccgtgagtct
    32701 cctttatagt gtgcaatcac ttcctcaagc gtttgcggct gttccggctt tgttaccccc
    32761 atccacgcat taataaggta attattgtaa gctgtcgaaa agatgttgtt ttccgttgtg
    32821 cgatccacgc catccccaag aacggggcta taccagataa acatcgggac atgatatgcc
    32881 tgctggctag cctccctgcc tccatgaaaa tagacgttct tcttcgtagg gtcacgttcc
    32941 aggccgtggt cggcaaaata catgaccgag gcgcggcgat cttttaataa ttcaaaaacc
    33001 tgacctagca aactatctgt gtaatgaatg gagttgtcat agcaggcatc ctgatcgtcc
    33061 tgcggttgaa acacggcgct ggattgcgga taggcgctac aagccggttc atggcttcca
    33121 tttaaatgaa gaacaatcag tttcttttgc tgcgtatttt gctgtaatgc ttgcgataaa
    33181 tgcggcaaca acaattcatc aaatcctctg acataaactg tttccatggc gcgcatggcg
    33241 atactggtaa ctgctgtacc attctgccga aaagcggatt gcgagcttag ccagaaagtc
    33301 tgaaatcctg cttgattagc catattaata atgttgtcgg ggtaattatg aatgtcatga
    33361 ctcaaaacag aatcagcagt taaagaaagg ggaaccgaca gcgcagtgta aggtgcgccg
    33421 cttattgctt gattaaacag tttgatctgt tttctttgtg cttcaacttg cggtgtcgta
    33481 gagcgtgtat atccatacaa agacatattg tcgacacgta cagactcccc cacaatcaac
    33541 acgtaggtat caatacctgt atccctgact gataattgaa aatacggcac cgtgtttgca
    33601 attgagagta atctttgatg ctctttcgct gctaaagcaa aatagttgag attgaaaaac
    33661 ggcgtatagg tagcaaatcg cgacgctagt atatatggac tgaacgcttt tttatttttt
    33721 gcatctttat aagcaaattg acaagcggaa aataaactgc ccgaaatgac aatcagcaat
    33781 aatattcctg tcactttttt tgtcggcaag gaaacatcat attttattat tactgccaaa
    33841 aaaagaaggg ataaaaaagc aaaggcacat agataaggga tatacatccc cagcattttg
    33901 acaacttcat ccggatcact ctggagcaca ctaatcgcaa atccatcatt gaaagttgta
    33961 ccaaaggtac accatgaata gaggctaata ctcatgtctg ccgcagtcag taacacaaat
    34021 ggtgcggcaa caagcaggcg aagcggaagg gatgatatac gttttacaac caggatggtc
    34081 agaacgaaga acagcaagcg ctgaaccata tgcgcgccgg aagaggcgac taagttcgta
    34141 ataccaaaaa caataacaca aagatacaac aaccaatgag atttaaaact tctagcaaat
    34201 ttgttgaata ctgtcatcca gaaatccaag ttcacaacca atataaacaa ttatcaacaa
    34261 tgttcaattt tatatcagac atgtttttca actctgatcc atatcagacc gaaacactgt
    34321 tagttttttc tttttaaatg atgcggccat tgcgttctca gtaattaaat aacattatca
    34381 atgcgtctct aaacattacg cagaacgtag ccatcagtag ttatactgac aggagcagga
    34441 tggtttatcg agaagtaaac atatccgtat tcgtaccgga actggagttg acacaataaa
    34501 gtgccaatta tgtcagcaga agggaaaagt ttataactaa ggcgtaccgg caccatcgtt
    34561 tcaaggtacc agctacgagt aaagcaactg gacgagatac agatacctga caaccattcc
    34621 tcagaccagg accaaaacga aaaaagacgc ttttcagcgt ctcttttctg gaatttggta
    34681 ccgaggacgg gacttgaacc cgtaagccct attgggcact accacctcaa ggtagcgtgt
    34741 ctaccaattc caccacctcg gcacggatac tactttttag ttcgggatat cgctggtcgg
    34801 cttagccgga gcagctggtt gagtttgttc ggttttcacc ggtgcactca gattttccca
    34861 ttcgctacct ttattggttt tgttgctatt gatgttaccc agcaccagac tgatgatgaa
    34921 gaataacgtt gccagcagcg ccgtcatgcg ggtcatgaag ttaccagaac cacttgaacc
    34981 aaacagcgta gcggaagcgc ctgctccgaa ggaggctccc atatcagcgc ctttaccttg
    35041 ctgcagcatg atcagaccaa caaggccaat tgccacaata aggaaaacta ctaaaagagc
    35101 ttcgtacata atcaacctgt tccttgcgga gttgccgcgt accaatgctt caaccaataa
    35161 agcgggagtt tttaatcttt cccactgaag cgggtgtgaa tactaaccaa agcgaatgac
    35221 cttcgcaagg gcaattttat cgcattgtat caactgcgga aaaaaacagc aaaaccgatg
    35281 tgttcgctga gaaaaaaggc gacaatcgcc gcctttttag ccagttatct aacgctttaa
    35341 acggctttta ctgcatcggc gatgcggtgt gcaaattcag tcacctgcgc ttcgtcttcg
    35401 ccttccacca tcacgcgaat taacggttcg gtgccggatt tacgcagcaa cacgcgtcca
    35461 cggttgccca gcgcagcttc aacctctgcg gtcacggctt taactgactc atgctcaagt
    35521 ggatcgccgc tacctgcggt gtaacgtacg ttaaccagaa tctgcgggaa cattttcatg
    35581 ccgctgcaaa ggtcgtgcag gctcatatgg ttacgtgcca tagccgccag cacctgcaag
    35641 ccagcaacga tgccgtcacc ggtagtagtt ttatccagca ggatcacatg accggaattc
    35701 tctgcaccga tacgccagcc tttctcctgc attttttcca gtacgtagcg gtcacccact
    35761 ttcgcgcgcg caaatggaat tcctaactgt ttcagcgcca gttcaagccc catgttgctc
    35821 atcaatgtac ccacagcgcc accacgcagc tggccctgac gaagaccttc acgcgcgatg
    35881 atatacatga tctgatcgcc atcgacttta ttgccttcat ggtcaaccat aatcacgcga
    35941 tcgccatcgc cgtcgaaggc aataccgaga tccgcttttt cagccagcac acgagcctgg
    36001 agcgcgcgaa cgtcggtagc ccccacttcg gcattgatgt ttacaccgtt tggctcacaa
    36061 ccgatagcga taacgttcgc ccccagttcg cgcagcacgt tcggcgcgat gtgataagtc
    36121 gcaccgtttg cacaatccac cacaatcttc agttcactga ggctaagttc gttcgggaac
    36181 gtggctttgc aaaactcgat atagcgaccc gcggcatcaa cgatacggct ggctttaccc
    36241 agttctgccg aatcaacgca gctgatctcc ttttccattt ccgcttcgat ggcctcttct
    36301 accgcatccg gcagtttggt gccgtcgata gagaagaatt taatgccatt atcgtagaac
    36361 gggttatgcg atgcagatat cacaattccg gcctctgcgc ggaaggtacg cgtcagataa
    36421 gccacggccg gtgttggcat cgggccagtg aagagtgcgg aaaggcccgc tgccgccaga
    36481 cccgcttcca gtgctgactc cagcatatag ccagaaatac gcgtgtcttt accaataatg
    36541 atcttacggg agccgtggcg cgccagcact ttacccgcgg cccaacccag cttaagcaca
    36601 aaatcaggtg tgatcggcgc atcccctaca cgaccacgaa tcccatcggt accgaaatat
    36661 ttacgattac tcatagcgtt tgttttcctt tgcagacaga gtggcttcca ccacccgcat
    36721 cgcttctacg gtttctttga cgtcatgaac acgaataatg tgcgcgcctt gcattgcggc
    36781 aatgaccgca caggccagac taccgctcag gcgctcggac ggccccacgt tcagcagctg
    36841 cccaatcatc gattttcgtg acatacctac caacagcggc aggttgaaat ggtgaaattc
    36901 agccaggcgc gccagtaatg aatagttatg ggagagattt ttaccgaaac cgaatccggg
    36961 gtcgagcaac aatttctctt ttgcgatacc cgcctgctcg caacgtgcta tttgctcaat
    37021 aaagtagcga ttcacttctg caaagacatc gtcatacttc ggagcttcct gcatggtttt
    37081 tggatttccc tgcatatgca tcagacaaac cggtaaaccg gtttctgcag ccgcctccag
    37141 agcgccaggt tcggaaaggg agcggatatc attaataatg tgagcgccaa ctttcgctga
    37201 ctcacggatg acttctggtt tggatgtatc taccgagatc cagacttcga agcgttgagc
    37261 aattgcctca accacaggaa taacacgttg caactcttct tcaacgctaa cttccgccgc
    37321 ccctgggcgc gtggactcgc caccaacgtc aatgatcgtc gcgccagcgt tgatcatcag
    37381 attcgcatgt ttcaccgcat ctatcagcga gttatgcgtg ccaccatccg aaaaggaatc
    37441 aggcgtgacg ttgaggatcc ccattacgtg aggatggcta aggtccagtg aagtaccctg
    37501 ggcaaagagt ttcatgatgt tatccctggt atgaattgat aagaaaaaac cccggagcac
    37561 gccccggggt tttcggtaca aatacagtca tctgatgcgg gaacttactt gtcgcctaac
    37621 tgctctgaca tggtgttacc cgggttcggc gtacgcggtt catcaaccgg acgaggagcc
    37681 tttggactac cattgtcgcc agaattgtta gaagcgcctg gttcttccca gcccgctggc
    37741 ggacgtacat cgcgacgtgc catcaggtca tcaatctgcg gtgcgtcgat agtctcatat
    37801 ttcatgagag catctttcat cgcatgcaga atatccatat tgtcggtcag aagctgacgc
    37861 gcacgattat agttacgctc aatcagtgct ttcacttcct ggtcgatgat acgtgcagtt
    37921 tcatcggaca tatgtttcgc tttcgctacg ctacggccga ggaacacttc accttcttct
    37981 tccgcgtaca gcagtggacc caatttctca gagaagcccc actgagtcac catgttacgt
    38041 gccaggttgg tcgcaacttt aatatcgttg gacgcaccgg tagatacatg ttccggcccg
    38101 tagatgatct cttctgccag acgaccaccg tacagcgtag aaatctggct ttccagtttc
    38161 tgacggctgg cgctgattgc gtcgccctca ggcaagaaga aagtcacacc cagcgcacga
    38221 ccgcgtggga taatcgtcac tttgtgcacc ggatcgtgtt ccggcaccag gcgaccgata
    38281 atcgcatgac ccgcttcgtg gtaagccgtc gattctttct gcgcttccgt catcaccatg
    38341 gagcgacgtt ccgcacccat catgattttg tctttcgctt tctcgaactc aaccatcgac
    38401 acaacgcgtt tgttgccacg agcagcgaac agtgccgctt cgttcaccag gttcgccagg
    38461 tcagcaccgg agaaaccagg agtaccacgg gcaatgattg ccgcgtcgat atcgggtgcc
    38521 aatggtacgc gacgcatgtg aactttcagg atctgctcac gaccgcgaac atctggcaag
    38581 ccgaccacaa cctgacggtc gaaacggcca ggacgcagca gggccgggtc gagaacgtcc
    38641 ggacggttag tcgcggcgat aacgatgata ccttcgttac cttcgaagcc atccatctca
    38701 accagcatct ggttcagagt ctgttcacgt tcatcgtgac caccgcccag accagcgcca
    38761 cgctggcggc ctacggcgtc gatttcatcg ataaagatga tgcacggtgc cgctttcttc
    38821 gcctgttcga acatgtcacg aacacgggat gcacccacac cgacgaacat ttctacgaag
    38881 tcagaaccgg agatagtaaa gaacggaact ttcgcttcgc ctgcaatcgc tttcgccagc
    38941 agcgttttac cggtacccgg aggaccgacc atcaagacgc ctttcgggat cttaccgccg
    39001 agtttctgga agcggctcgg ctcgcgcaga tactcaacca gttcagcaac ttcttctttt
    39061 gcttcgtcgc agcccgcaac gtcagcaaag gtcgttttga tctgatcttc cgtcagcatg
    39121 cgcgctttgc tcttaccaaa cgacatggca cctttgccac cgccgccctg catttgacgc
    39181 atgaagaaga tccagacacc aatcagcaac agcatcggga accaggagat gaagatagaa
    39241 gccagcaggc ttggttcttc aggcggttca ccgacaacct tgacgttctt ggtcaacagg
    39301 ttatccagta atttcggatc ctgaaccgga atgtaagtgg tataacggtt actatctttc
    39361 ttggtaacgt tgatttcacg tccgttgata cgcgcttcac gaacctggtc gttattgacc
    39421 tcttgtagga aggtagagta atccacctta cggccattag actcgctggg cccaaagctc
    39481 tggaatactg acatcagcac aacggcaatg accagccaga gtattaggtt tttcgccatg
    39541 tcactcaagg gattaacctc ttattacaac tgtgttaaaa acagcgtcag gatactctat
    39601 atccagcatc tttcaaactt tcgtctgaaa tctcccggtt agggtttacg cccggtcgct
    39661 acaatataca cttcccgcga acgtgcacga gaagagtccg gcttacgaac tttgaccttc
    39721 gtaaacaggg agcgaatttc ccttagatac tcatcgaaac cttcgccctg gaacaccttc
    39781 actacaaaac tgccacctgg cgctaataca tcacgacaca tttctagcgc cagttccacc
    39841 agatacatgg cacgggggat atccaccgcc ggtgttccgc tcatgtttgg tgccatatcg
    39901 gacatgacaa cctggacttt gctgtcgcca acgcgctcca gcagtgcttt catcaccagt
    39961 tcatcacgaa aatcgccctg aagaaagtcc acaccaacga taggatccat aggtaaaaga
    40021 tcgcaagcga tgatgcggcc tttgccgcca atttgggtga ccacatattg tgaccaacca
    40081 cccggagcag caccaaggtc gacaaccgtc attcccggtt taaagagttt gtcactttgc
    40141 tgtatttcat caagtttaaa ccaggcacgg gaacgtaacc cctttttctg tgcctgttga
    40201 acatatttat cgctaaagtg ttcctgaagc cagcggctgg agctggcaga acgcttctta
    40261 cctgtcattt aactttccca tggggataac tcatcgtaac caattgcgta aatttttacg
    40321 cgcctatttg gtgatatatg ggagatggcg gtagaatgac ccgttttcaa tcccaacgta
    40381 agcaaaaaat acgatgaatc tgagtactaa acaaaaacag cacctgaaag gtctggcaca
    40441 tccgctcaag ccagttgttc tgcttggcag taatggtttg accgaagggg tgctggccga
    40501 gattgaacaa gcgttagagc accatgaact catcaaggtg aaaatcgcca ccgaagatcg
    40561 cgaaactaaa accttgatcg tggaagctat cgtgcgcgaa accggcgcct gtaatgtaca
    40621 ggtcatcggt aaaacgctgg tgctttatcg cccaactaaa gaacgtaaaa tctcgctgcc
    40681 acgctaagat tatcctaaag ttacacacat ttgctgtgta aaacgagggg ttttccgcag
    40741 gcaggagagc aaaatgccac gctctgttcg ttgataaaag gccgcatagc ggcctttttc
    40801 ctttctttac aatacatcaa catcttgagt attgggtaat tcttacaggt attccacctt
    40861 aattacttca aattctactt cgccgcccgg cgttttgatg accacaacat catcttcttc
    40921 tttgccgatc aggccacgag caataggcga gtttacagaa atcaggtttt gtttaaagtc
    40981 agcttcgtca tcgccaacga tgcgataagt ctgttcttcg tcagaatcga gattcagcac
    41041 cgttacggta gcaccaaaaa taacgcgccc attgttgggc attttggtga catcaatcac
    41101 ctgcgcgttt gacagcttgg cttcgatgtc tttgatacgg ccttcgcaga agccctgctg
    41161 ttcacgagct gcgtggtatt cggcgttttc tttcaggtcg ccatgctcac gcgcttccgc
    41221 gatagcagca atgatttcag gacggcgcac agatttcaga aaatccagct cttcgcgtaa
    41281 tttttcagcg ccgcgtaagg tcatcggaat agcttgcatt tgttatacct cttgaatatt
    41341 cctgatgggg caagtcttca cccaccccgg ctgttcggcc tgcccggcat aactcctcac
    41401 cgggaccaga agcaaaaaaa taccgacccg ggtacaagtc ccaggtcagc tacaattcac
    41461 attttgatag tcattttacc ctgaagttcc cgaagggtca tcgtttactt tatagggcgt
    41521 tgcgccgtag tatgacggct cgattccagg ttgttagcgc gagattatgc gattttccag
    41581 atttatcatc ggattgacca gctgtatagc gttcagtgtt caggccgcaa atgttgatga
    41641 gtatattact caactccccg ctggtgccaa ccttgccctg atggtgcaaa aagtcggcgc
    41701 gtcggccccc gctattgatt accacagtca gcagatggcg ctgcccgcca gtacgcagaa
    41761 agtgattact gcgctggcgg cgttgattca actcggcccc gattttcgtt ttaccacgac
    41821 gcttgaaacc aaaggcaatg tggaaaacgg cgtacttaag ggtgacttag tggcgcgatt
    41881 tggtgccgat ccgacgttaa aacgtcagga tattcgcaat atggtcgcga ctttgaaaaa
    41941 atctggcgtc aaccaaatcg atggcaatgt gttgatagat acctccattt tcgccagcca
    42001 cgataaagcc cccggctggc catggaatga catgacacaa tgctttagcg ctccgcctgc
    42061 cgccgccata gttgaccgca actgtttctc cgtctcgctc tacagtgccc aaaaacccgg
    42121 tgatatggcc tttatacgcg tggcatctta ttaccccgtt acgatgttca gccaggtacg
    42181 caccctcccc cgtggttctg ccgaagcgca atactgcgaa ctggatgtgg tgccaggcga
    42241 cctgaaccgc tttacgctga cgggatgcct gccacaacgt tctgagccgc tcccgttggc
    42301 ttttgccgta caggatggag ccagctatgc tggggcaatt ctgaaagatg agttaaaaca
    42361 ggcgggtatc acctggagtg gaacactgct gcgccagact caggttaacg aacctggaac
    42421 ggtagttgcc agtaaacagt cggccccgct gcacgatctg cttaagatta tgctgaaaaa
    42481 gtcagacaac atgatcgccg atacggtttt ccgcatgata ggccatgcgc gcttcaatgt
    42541 gcctggaaca tggcgggccg ggtcggacgc cgtgcgtcag atcctgcgcc agcaagccgg
    42601 tgtcgatatt ggaaacacca ttattgccga tggttcaggg ctttcgcggc ataacctgat
    42661 tgcccccgcc accatgatgc aggtgctgca atacattgcc caacacgaca atgaacttaa
    42721 ctttatctcc atgctgccgc tggcgggcta tgacggctct ttgcagtacc gtgcaggtct
    42781 gcatcaggcg ggcgtggatg gcaaagtctc agcgaaaacc ggttcgttgc agggggtata
    42841 taacctggcg ggattcatta ccacagcgag cgggcaacga atggcgtttg tgcaatatct
    42901 ttctggctat gcagtagaac cggcggatca gcgtaatcgc cgtattccgt tagtgcgttt
    42961 cgaaagccgt ttgtataaag atatttacca gaacaattag tcaaaaagaa accccggcat
    43021 atggccgggg tttcagatta ttgacaaagt gcgctttgtt tatgccggat gcggcgtgaa
    43081 cgccttatcc ggcctacaaa atcgtgcaaa ttcaatatat tgcaatcatc ttgtaggcct
    43141 gataagtgta gcgcatcagg cttatttggc attggtcatt agtgattaac gcttgtaaat
    43201 gaactcaacg ccttcttcgt cgtcttcgtc ccagtcgtca tcccagtcct catcatcctc
    43261 ttcagcaatc tcttcaagct gctggcgatg ataatcatcc cacatgaatt cgactttctc
    43321 tggctgtttc gcttcttcag cctgcacgac cgggttttca atgataaagg tcatcacatc
    43381 ccagcagaga tctttcacgc ccagtccgct cgccgcagag atcagataat atttatcttc
    43441 ccagcccagc gcctcagcga tcgctttcgc tttctcttcg gcttctgcct tatccagcag
    43501 atcgatcttg ttgaacacta accaacgcgg tttcgccgcc agatcctggc tgtatttttc
    43561 cagctcgctg ataataatac gcgcgttttc aaccggatcg gtgccgtcaa tcggatcgat
    43621 atcgatgagg tgcaacagga cgcggcaacg ttccaggtgc ttcaggaagc ggatacccag
    43681 acctgcgcct tccgcagcgc cttcaatcag tcctggaata tcggcaacaa cgaagctctt
    43741 ttcgttgtcc attcgtacca cacccagact tggcaccaga gtggtaaacg gataatccgc
    43801 cactttcggt ttagccgccg ataccgcgcg aataaaggtc gatttccccg cgtttggcat
    43861 ccccaacata ccgacgtcag ccagcagcat cagctccagc agcagctcac gcttatcacc
    43921 cggcgtaccg ttggttttct gccgtggtgt acggttaacg gacgatttga aacgggtatt
    43981 gcccagaccg tgccagccgc ccttagcaac cagcagacgc tgaccgtgtt tagtcatatc
    44041 gcccatggtt tcaccagtac cctggtcgat tacacgcgta cctaccggca ctttaatcgt
    44101 cacgtcttta ccgcgcttac cggtacaatc gcggcttgcg ccgttctgac cgcgctctgc
    44161 acggaaagat ttttcaaaac gataatcgat aagcgtgttc aggttttcgt cggcttccat
    44221 ccatacgtca ccaccatcac caccgtcgcc gccatccggg ccgcctttcg gaatatactt
    44281 ttcgcggcgg aagctcacgc aaccattacc gccatcacct gcaacgacca gaatcgatgc
    44341 ttcatcaaca aacttcattt tattctccgt aaatcattcg cctgcgcggg gttgcgatac
    44401 caccgttgta tgcttacgta atccgcccca aatacgatga ccaatggcgg aatacatcgc
    44461 gcccgcaacc acgacaaacg caccgagata acctaaaagg tttaacatcg gtctggcgaa
    44521 gaaatcgggc caggccagtg ataaaagatc tgaaaaaaac agcgtaaaca gtggggtgag
    44581 cgtgatgatc gcgctcacct gcgctgccag ccagcgagcc atcgcttccg ccagggcgcc
    44641 atatcctacc aatgtattca gtccgcaaaa aattaaacat gcgagctgcc agtggctaag
    44701 ctgcgctatc actccaggct ttgccagagg gaagagcgcg attgtacata aagtgtacag
    44761 caaaaacagg atctgcggtg aggccagccg acgcaataaa accttttgcg ccacgccata
    44821 actcacccaa accgtcgccg caccgacccc aaagatgact ccccaggtgt aatcagtgag
    44881 ctttgtaaat atctcgacca gactggtgtt aaaaaacatc accaggccgc tcaggagcat
    44941 caatgcccct acaacctgag tgctgcgcat tttctctttc aggataaata cgctggcaac
    45001 catcatgcca actggcgaga gttgcccaat cacctgcgaa gcggtcggac tcaggtattg
    45061 caaggatgag ctgaacagga tgaagttccc aaacagcccg gcggtcgcca ctgccaacaa
    45121 aatcaaccag cgtggcttac gaaacacgcg taatggcggc aacctcttct tcaccgcaag
    45181 aatggcaccc aggccaatac tcgccatcaa gaaacggtaa aacacgattg tcggaggttc
    45241 catcacctcc agcacctgct tcattgcgat tggcaacgcc ccccagcaaa ttgctgtggt
    45301 gagcgccaaa agaatgccaa tgcctgcctg ctgcttcata ccgtattccc tgcaaagcgc
    45361 atttgcggat taacgtcgca ccatgctcat ttttaccggt tatcgaatgt aaaaagcccc
    45421 gcaacgtgtt gcggggcttt catccgttac cgggacgcga aaaacttatt cagcttcgat
    45481 gctgataaat ttacggtttt tcgggccttt aacttcgaat ttcactttac cgtctgcttt
    45541 agcaaacaga gtgtggtcac gaccgcaacc tacgttagcg ccagcgtgga atttggtacc
    45601 acgttgacga acgatgatgc tacccgccag aacggattcg ccaccgaaac gcttaacgcc
    45661 caggcgttta gcttctgaat cgcgaccgtt acgtgtggag ccgccagcct ttttatgtgc
    45721 catttgaaat ctctcctcag gtcttaggcg ctgatgccag taattttcac atcagtgaac
    45781 cactgacgat ggccctgctg cttacgatag tgtttacgac gacgaaactt aacgatttta
    45841 actttctcgc cacgaccgtg agcaacaact tcagctttga ttacgccgcc atcaacgaaa
    45901 ggaacgccga ttttgacttc ttcaccgttt gcgatcatca gcacttcagc gaactcaaca
    45961 gtttcgccag ttgcgatgtc cagcttttcc aggcgaacgg tctgaccttc gcttactcgg
    46021 tgttgtttac caccactttg gaaaaccgcg tacatataaa actccgcttc cgcgcacacc
    46081 ttttcaatga ttcagagtgc gctataaata ttcacaatag ggcgcgaata ttacgcaaaa
    46141 cgcacgcctt tgacaagtgc tacagtcaat acacgaagaa aaaaaacaca acttgtacgg
    46201 taacgtttat ctgtgccatt ttttcagtac aatcacccta tattcctaac cataaaccct
    46261 aagttgcctt tgttcacagt aaggtaatcg gggcgaaaag cccggctttt gcgatgaatt
    46321 tagaaaaaat caatgagtta accgcgcaag atatggcggg tgttaatgcg gcaatccttg
    46381 agcagcttaa ttccgacgtc caactgatca atcagttagg ctattacatc gtcagcggcg
    46441 gcggtaaacg tattcgtccg atgattgctg tactggctgc acgagctgtt ggctatgagg
    46501 gaaatgcgca tgtcaccatt gctgccttga tcgagtttat ccacacggcg actctgctac
    46561 acgacgacgt tgtggatgaa tcagatatgc gcaggggtaa agctaccgcc aacgccgcat
    46621 ttggcaatgc cgccagcgtg ctggtaggcg attttattta tacccgcgct ttccagatga
    46681 tgaccagcct cggttcactc aaagtgctgg aagtcatgtc agaagccgta aacgtcatcg
    46741 cagaaggtga agttctgcaa ctgatgaatg ttaacgatcc ggacatcact gaagaaaact
    46801 acatgcgcgt tatctatagc aaaaccgcgc gtctgtttga ggctgccgcg cagtgttccg
    46861 gaattctggc tggctgtacg ccggaggagg agaaaggcct gcaggattat gggcgctatc
    46921 tcggcactgc tttccagttg atcgacgatt tactcgatta caatgccgat ggcgaacagt
    46981 taggtaaaaa cgtcggcgac gatctgaacg aaggtaaacc gacgctgccg ctgctgcatg
    47041 cgatgcatca tggcacacca gaacaggcac agatgatccg taccgccatc gaacagggta
    47101 acggtcgcca tcttctggaa ccggttctgg aagcaatgaa cgcttgtgga tctcttgaat
    47161 ggacgcgtca gcgcgccgag gaagaagcag acaaagccat cgcagcgtta caggtgctcc
    47221 cggacacccc ttggcgagaa gcactcatcg gcctcgcgca catcgctgtt caacgcgatc
    47281 gttaatcccc tcccctcatc ccgcgcagtg cgcgggatga ttccagtaaa ctccataaga
    47341 atacttattc agcgtaataa tgcactgaaa aaccagtgta tacatcttta tatattctga
    47401 atattcacac tctttacagg aactttttag agcaatcggc catcaggagt atagtgatgc
    47461 tcgacagaag aagtgttctg aatgaaagcg actaacttaa ggagtgagga aagatggaaa
    47521 gtaatttcat tgactggcat cccgctgaca tcattgcggg tttgcgcaag aagggaacgt
    47581 caatggcggc ggaatctcgc agaaatggtt tgagttcctc aacgctggcg aatgcattat
    47641 cgcgcccatg gccgaaagga gagatgatta ttgcgaaggc cctgggaact gacccctggg
    47701 ttatctggcc atcacgctac catgatccgc agacccatga gtttatcgac agaacgcagt
    47761 tgatgcgtag ctacactaaa ccgaaaaaat gagtggtctg gcggtagccc cgcgtacggg
    47821 gctgccagct ttcagacgat tactcgcctt tcacacgctc aatatttgca cctaaagcgc
    47881 gcagtttgtc ttcaatgcgt tcgtagccac gatcgatgtg ataaatacga tcaaccaccg
    47941 tcgtcccttc cgcaatacag ccagccagca ccaggcttgc tgatgcacgc agatcggttg
    48001 ccataacctg tgcgccagaa agtttttcaa caccgtgaca aataacggta ttgctttcga
    48061 tttcggcgtg cgcgcccata cggctcagct ctggcacatg cataaagcgg ttttcaaaga
    48121 ccgtttcggt gatgaatcca gtcccttctg ccaccaggtt caacagcgtg aactgggcct
    48181 gcatatcggt cgggaatgcc ggatgcggcg cggtacgtac gttaacagcc ttcggacgtt
    48241 tgccatgcat atccaggcta atccagtctt cgccgacttc gatgtccgct ccagcgtcac
    48301 gcagtttcgc cagcacggcg tcgagagtat ctggctgcgc gttacggcag ataattttgc
    48361 cgcgagaaat cgccgccgcc accaggaatg taccggtttc gatacgatcc ggcagcacgc
    48421 gatagacacc gccgcctaaa cgttccacac cttcgatgac gatacgatcg gtgccctgac
    48481 cgctaatttt cgcacccagc gtaatcagga agttcgcggt atcgacgatt tccggttcac
    48541 gcgctgcgtt ttcaataatc gtggtgcctt ccgccagggt tgcagcacac atgatggtca
    48601 ccgttgcgcc aacgctgact ttatccatca cgatatgtgc gcctttcaga cgaccatcga
    48661 cggaagcttt aacgtaacct tcttccagtt tgatggtcgc gcctaattgt tcgaggccag
    48721 aaatgtgtag atcaaccgga cgcgcaccga tcgtacaacc gccaggtagt gaaacttgcc
    48781 cctgaccaaa gcgcgctacc agcggcccca gtgcccagat agaagcacgc atggttttaa
    48841 ccagatcgta aggtgcgcag aatacattaa cgtcgcgggc atcaatatgc acagaaccat
    48901 tacgttctac tttcgcaccc agctggctta gcagcttcat tgatgtatcg acgtctttca
    48961 gtttcgggac gttctggatc tctaccggtt cttccgccag tagagcggca aaaaggatag
    49021 gcagagcagc atttttagcg ccggaaattg tgacttcgcc ctggagcttc gttggcccct
    49081 gaacacgaaa tttatccatt tagtttgttc tcagttaaca attcatatcc gctaccggcg
    49141 aatcgcccat agctcaaaag ccgttcagtt tgcgatcgcg cgcccactcc gcaggggtat
    49201 acgctttgat cgacacagca tgaatgcggt tatccgcaat atattccatc agcggaccat
    49261 agaccgtctg ctgtttttta acccgactca tgccgtcaaa caactcaccc acggcaataa
    49321 cctgaaagtg gctgccatcg ccggaaacgt ggacttcctg gagggagaga gcgttcatca
    49381 acacgctctg aatttcatta ttttccatgg gatcttcaat catcagttaa taaaccagcg
    49441 aaacatctta gagcaaagtt gcgctggcat aaataagcaa aaagcctcgc tgataaatca
    49501 gacaaggctc gacttgcagg caggtttgcc ggacaggcgg ttaacgccat atccggcctg
    49561 aaaaaattta acgaggcaga acatcagcag gcaaattata caatttcgcc agggtataca
    49621 ctttgtcgtt taccccctga agcgtcacat tgttgccctg ctttttcgcc agatcgataa
    49681 gatggagcag cagtgccagt ccccccgtat ccacgcggga gacacggcta agatcgatgc
    49741 aggtaatccc cttcaccgct tcctcacgca tttcccaaag cggtagcaaa acgtcctgat
    49801 ccagctctcc ggataacgcc agcgtgtcac ccgtctgcat ccagctcagt gactcgctca
    49861 ttattttttc tcttccagag tgattttctg ttgagaaatc gatttcagtt gcgcagtcag
    49921 gccgtcgata cctttggtac gcagcagcgt tccccactcg ttttgtttgg tggtgatcat
    49981 actgacgcct tcagcaatca tgtcgtaagc ctgccaattg cccgtctggg agtttttacg
    50041 ccactggaag tccagacgca ccggcggacg gccattcggg tcaataatgg taacgcgaat
    50101 aggcacaatg gttttatcgc ccagcggctg ttctggcgca atctgatagg tttgaccgtg
    50161 atacatcgcc agcgcctgac cgtaagcctg cttcaggtac tcacggaaag cggcaaagta
    50221 ggcttcacgt tgagcagggg tcgcactctt gtaatactgg cccagcacca gcgcaccggc
    50281 gtatttcacc tgtacgtatg gcagcagttc ctgatcaaca atggtacgca gataatccgg
    50341 gttggcccga atttgcggtt gctcattctt caggcgatcg aacgttttct gcgccgcctc
    50401 gtccatcagc ttatacggat tggtctggtc tgccgcggtt gccgcactca gaggtgcaat
    50461 caccagcaaa gcgaccatca ttaaacgttt aaacatgcgt cggttctcct gaaattattt
    50521 cgttgtaccc acaggttcag tggtttcatt attacctggc gcagcagctg gcgcatcgcc
    50581 actattctta ttgtcatcgc ctttactacc gtaaaggaac tgaccaatga gatcttccag
    50641 caccatcgca gacttagtgt cctgaattgt atcgccatcc ttcaggatag cagtccccag
    50701 ttccgggtct tcaaaaccga cgtttaatgc cagatattgt tcccccagca ggccggaagt
    50761 acgaatgctc agcgaactgg tatctggaat gtggttataa cgttgttcaa tttccagcgt
    50821 tacgcgcggc agataggttt tcgggtccag cgtaatatcc gccacccgac ccacaacaac
    50881 gccaccaata ctgaccggag agcgcgcttt caggccgcca atgttatcga acgtcgcata
    50941 aagcgtgtag gtcggttcag tacgtatgga cgtcacgttc gccgccttca ggcaaacaaa
    51001 cagcgccgcc agcagtgctg ctaataaaaa gatacccacc caaatttcat tttttttcgt
    51061 ttgcatgaac tcaattccca aacatcaatg cggtcagcac aaaatccagc cccagaacag
    51121 ccagagacga gtggacaacg gtgcgagtgg ttgcccggct aatcccggca gacgtcggga
    51181 tggcgtcgta gccgttaaac aacgaaatcc acgtcaccgt gatggcgaac accacgctct
    51241 taatcagaca gttgaccaga tccatacgcc agtcgacggc attttgcatt gccgaccaga
    51301 agaacccgct atcaatgcct ttccaactga cgccgaccag agatccgccc cagatcccca
    51361 cggcgacgaa aataaccgtc aacagtggta atgaaataac cccagcccag aaacggggag
    51421 aaataacccg acgcagcgga tccaccgcca tcatctccat actggagagt tgctctgtag
    51481 cgcgcatcag gccgatttct gcggttagcg ccgaaccagc acgcccggca aacaacaacg
    51541 cggcaaccac cggccccagt tcacgcagta gcgataacgc caccagcata cccagactgg
    51601 tttccgcact ataagtggtc agaaccagat aaccttgcag ccccaacacc attccgatga
    51661 acacgccaga aaccacaata atcagcatcg acaggacgcc gacattatag agctggcgca
    51721 ccagcagcgg cgcatgtttg cgaaattccg gtttgccgac cagcgcattg aataacatta
    51781 acccggcccg cccgaacgtt ctcagggttt taatcccttt atgtccgagc gacgccagcg
    51841 catttaacag catgagtggc ttaactccct ggtaaaagat cagcgtgata atcgccggca
    51901 ggatagcgga acggaacagg cccgtcagct atcccgtcca gaaactgacg tacgcgcgga
    51961 tcaggattcg cctgcaacgc ctgggcactg ccatgagcga caattttttt gtccgccagg
    52021 atccaggcgt gatccgcaat acttaacact tccggcacat cgtgagaaac caccacacaa
    52081 gtcacgccca gcgcgctgtt cagctcagaa atcagcttca ccagtacgcc catggtgatg
    52141 ggatcttgcc caacaaaagg ttcatcaaac atgatgagat ccggctccag cgcaatcgca
    52201 cgcgccagcg ctgcacgccg cgccatccca ccggaaagtt cagaaggcat tagtttagcc
    52261 gctccacgca gccccacggc ctccagcttc atcatcaccg tactatgcaa caatggcgcg
    52321 ggaagttggg tatgttcgcg cagtggatag gcgacgttgt caaatacgtt catatcagtg
    52381 aacaacgccc cggactgaaa taacatgctc atccgtttgc gcactgtata caggcgcgaa
    52441 cgagacatcg ccggaatatt ctcaccatcg aaaaggatct caccatgatc tggtgcgatt
    52501 tgcccgccaa tcagacggag tagcgtcgtt ttaccgatgc ccgatggccc catgatcgcc
    52561 gtgatcttcc ctcgcggcac ggtcagggaa atattatcga agatgcagcg attgccacgc
    52621 gtaaaactga catcgcgcat atcgactaaa ttcgccacag actgctccat aattcaccct
    52681 tcgtcttgcg ttgattttct aagcatggcg ctcaatttaa ccttgaaccc aacatattta
    52741 cagaatatta cccgccgtgg ttagcgaaag ctggcatttg ttttactttt tagccgcata
    52801 aagtcaaaat taagaatccg ttacggcttt ctgaaaatct tcagcggacc ggcgagtata
    52861 cctgaagaaa ggacgttaga tgcttttagc tacggcactg ttaattgttg gtttactttt
    52921 ggtcgtttac agtgccgacc gcctggtttt tgccgcgtct attctttgcc gaacctttgg
    52981 catcccgccg ctgatcatcg gcatgacggt ggtcagtatt ggtacatcgt taccagaagt
    53041 catcgtctcg cttgccgcgt ctctgcacga acaacgcgat ttagccgttg gtacagccct
    53101 cggctcaaac attatcaata tattgctgat cctcggtctg gctgcgctgg ttcgtccttt
    53161 taccgtccat tctgatgttc tacgccgtga attaccctta atgttgttgg tcagcgtggt
    53221 ggccggttcc gtactctatg acggacaact tagtcgcagc gatggtatct ttctcctctt
    53281 tctggctgtg ctatggctgc tgttcattgt taaacttgca cgtcagggga ctgacagcct
    53341 gaccagagag cagcttgcag agctgccgcg tgacggcgga ttgcccgtcg cgtttttatg
    53401 gctcggcatt gcgcttatca tcatgccagt ggccacgcgg atggtggttg ataacgccac
    53461 ggtgctggcg aattactttg ccatcagcga gttgacgatg ggtctgacgg caattgctat
    53521 cggaaccagc ctgccggaac tggcaaccgc aatagcgggg gttcgcaaag gtgaaaacga
    53581 cattgctgtc ggaaatatca ttggcgcaaa catttttaat attgtcatcg tgttgggttt
    53641 acccgcgctg ataacgccag gagagattga tccactggcg tacagtcgtg actacagcgt
    53701 gatgttgctg gtgagcatta tttttgcgtt gctgtgctgg cggcgctccc cgcaaccggg
    53761 ccgtggtgta ggggtattat taactggcgg atttatcgta tggctggcga tgttgtactg
    53821 gttatcgcca atactcgttg aataactgga aacgcattat gtcgcacgta gagttacaac
    53881 cgggttttga ctttcagcaa gcaggtaaag aagtcctggc gattgaacgt gaatgcctgg
    53941 cggagcttga tcaatacatc aatcagaatt tcacgcttgc ctgtgaaaag atgttctggt
    54001 gtaaagggaa agttgtcgtc atggggatgg gaaaatcggg gcatattggg cgaaaaatgg
    54061 cggcaacgtt tgccagcacc ggtacacctt catttttcgt ccatcctggt gaagccgcgc
    54121 atggtgattt aggcatggtt accccacagg atgtggtgat tgctatctct aactctggtg
    54181 aatccagcga aatcacggcc ttaattccag tgcttaagcg tcttcacgta ccgttaatct
    54241 gcatcaccgg tcgcccggag agcagcatgg cgcgcgccgc agatgtgcat ctgtgtgtta
    54301 aagtagcgaa agaagcctgt ccgttagggc tggcaccgac cagcagcacc accgccacgc
    54361 tggttatggg cgatgccctc gctgtcgcgc tgttaaaagc acgcggcttt actgctgaag
    54421 attttgcgct ctcacaccca ggcggcgcac tgggtcgtaa acttctgctg cgcgtaaacg
    54481 atattatgca tacgggcgat gagatcccgc atgttaagaa aacggccagt ctgcgtgacg
    54541 cattgctgga agttacccgc aaaaatcttg gtatgactgt catttgcgat gacaatatga
    54601 tgattgaagg catctttacc gacggtgatt tacgccgtgt cttcgatatg ggcgtggatg
    54661 ttcgtcagtt aagtattgcc gatgtgatga cgccgggggg aatacgtgtg cgccctggca
    54721 ttctggccgt tgaggcactg aacttaatgc agtcccgcca tatcacctcc gtgatggttg
    54781 ccgatggcga ccatttactc ggtgtgttac atatgcatga tttactgcgt gcaggcgtag
    54841 tgtaaagatt caaggataaa caacaatgag caaagcaggt gcgtcgcttg cgacctgtta
    54901 cggccctgtc agcgccgacg ttatggcaaa agcagagaac attcgtctgc tgatcctcga
    54961 cgttgatggc gtactgtcag atggcctgat ttatatgggc aataacggcg aagagctgaa
    55021 agcgttcaat gttcgtgacg gttatggcat tcgttgtgcg ctcacctctg atattgaagt
    55081 cgctatcatt accgggcgaa aggctaaact ggtagaagat cgttgtgcca cattggggat
    55141 cactcacttg tatcaggggc agtcaaacaa actgatcgcc tttagcgatc tgctggaaaa
    55201 actggcgatt gccccggaaa atgtggctta tgtcggcgat gatctcatcg actggccggt
    55261 aatggaaaaa gtgggtttaa gcgtcgccgt ggccgatgcg catccactgt tgatcccgcg
    55321 cgccgattac gtgacgcgca ttgctggcgg tcgtggcgca gtgcgcgaag tttgcgactt
    55381 attactcctg gcgcagggca aactggatga agccaaaggg caatcgatat gagtaaagcc
    55441 agacgttggg ttatcattgt gctatcactg gcggttctgg tgatgatcgg cattaatatg
    55501 gccgaaaaag acgataccgc ccaggtggtc gtcaacaaca atgatcccac ctataaaagc
    55561 gagcatacgg acacgctcgt ctataaccca gaaggggcac taagctatcg attgattgct
    55621 caacacgttg aatattattc cgatcaggcc gtttcgtggt ttacgcagcc ggtacttacc
    55681 acgtttgata aggataaaat cccgacatgg tccgtaaaag cagataaagc caagctgacc
    55741 aatgaccgga tgctctattt atatggacac gttgaagtca acgcacttgt gccggactct
    55801 caacttcgca gaatcacgac ggataacgcg cagatcaatc tggtgacgca ggatgttacc
    55861 tctgaagacc tcgtcacgtt atacggaaca acatttaact ccagcggtct gaaaatgcgc
    55921 ggcaacttac gcagcaagaa cgccgagctg attgaaaagg ttagaacatc ctatgaaatt
    55981 caaaacaaac aaactcagcc ttaatcttgt gcttgccagc tcacttctgg ccgccagcat
    56041 tccggcattt gccgtaaccg gagacactga tcagccgatc cacattgaat cggaccagca
    56101 atctcttgat atgcaaggca acgtggttac ctttaccggt aatgtcatcg tcacccaggg
    56161 caccatcaaa attaatgccg acaaagtggt cgttacccgt ccgggcggcg aacaaggtaa
    56221 agaagtgatt gacggctacg gtaaaccggc aacgttctac cagatgcagg acaacggtaa
    56281 acccgttgaa ggtcacgctt cccagatgca ctacgaactg gcaaaagatt ttgtcgttct
    56341 gacgggtaat gcttatctgc agcaggtcga tagcaacatt aagggcgata agatcactta
    56401 cctggtgaaa gagcagaaaa tgcaggcttt cagcgacaaa ggcaagcgcg taaccaccgt
    56461 tctggtgccg tcgcagctgc aggataaaaa caacaaaggc cagaccccgg cacagaagaa
    56521 gggtaattaa ttcgttatgg caacattaac tgcaaagaac cttgcaaaag cctataaagg
    56581 ccgtcgcgtg gtagaagacg tcagcctgac cgtcaactcc ggggaaatcg tcggtctgct
    56641 ggggccaaac ggtgccggta agaccaccac tttctacatg gttgtaggca ttgtgccgcg
    56701 cgatgcgggc aacatcatta ttgatgatga cgatatcagt ctgctgccac tgcatgcacg
    56761 cgcgcgccgc ggtatcggct atctgccaca ggaagcctcc attttccgtc gcctcagcgt
    56821 ttacgataac ctgatggcgg tactgcaaat tcgtgacgac ttgtctgctg aacaacgtga
    56881 agaccgcgcg aacgagctga tggaagagtt tcacattgag cacctgcgtg acagcatggg
    56941 gcagtcactc tccgggggtg aacgtcgccg tgtagaaatt gcccgcgcac tggctgcgaa
    57001 tccgaaattt attctgctcg acgaaccgtt tgccggggtt gacccgatct cggttatcga
    57061 cattaaacgc atcattgagc acctgcgcga cagcggcctg ggcgtgctga tcactgacca
    57121 caacgtgcgt gaaacactgg cggtttgtga acgcgcttat atcgtcagtc aggggcattt
    57181 gatcgcccac ggcacgccta cagaaatctt acaagacgaa cacgttaagc gtgtatacct
    57241 tggggaagac ttcagactct gatagggtag aagtttgcga cgttttagca ggagagtacg
    57301 attctgaaca tgaagcaagg tttgcaactc aggcttagcc aacaactggc gatgacgcca
    57361 cagctccaac aggcaattcg tctgttgcag ttgtcgacgc tggaacttca gcaggagcta
    57421 cagcaggcgc tggagagtaa tccgctgctt gagcaaatcg acactcatga agaaatcgac
    57481 acccgcgaaa cgcaagacag tgaaacgctg gacaccgccg acgcgctcga acaaaaagag
    57541 atgccggaag agctgccgct cgatgccagt tgggacacca tttacaccgc tggtacacca
    57601 tccggcacca gcggtgacta cattgacgat gagctgccgg tctatcaggg cgaaacgacg
    57661 cagaccttgc aggattacct gatgtggcag gtcgagctga caccgttttc cgacactgac
    57721 cgcgctattg ctacctctat cgtcgatgcc gttgatgaca ccggttatct gactgtcccg
    57781 ctggaagata ttctcgaaag tatgggcgat gaagagatcg acatcgacga ggttgaagcc
    57841 gtccttaagc ggatccaacg gtttgatccg gtcggtgtag cggcaaaaga tctgcgtgac
    57901 tgcctgctaa tccaactctc ccaattcgat aaaaccacgc cgtggctgga agaggccaga
    57961 ttgatcatta gcgatcatct cgatctgtta gccaatcacg acttccgcac tttaatgcgc
    58021 gtcacgcgtc tgaaagaaga tgtgctgaaa gaagccgtca atctgatcca gtcgctcgat
    58081 ccgcgccccg ggcagtcgat ccagactggc gaacctgagt atgtcattcc agatgtgctg
    58141 gtgcgtaagc ataacggtca ctggacagta gaactcaaca gtgacagcat tccgcgtctg
    58201 caaatcaacc agcactacgc ctcgatgtgc aataacgctc gcaatgatgg tgacagccag
    58261 tttatccgca gcaatctgca ggatgccaaa tggttgatca agagtctgga aagccgtaac
    58321 gatacgctac tgcgcgtgag tcgctgtatc gttgaacagc agcaagcctt ctttgagcaa
    58381 ggcgaagaat atatgaaacc gatggtactg gccgatatcg cccaggctgt cgaaatgcat
    58441 gaatcgacga tatctcgcgt gaccacgcaa aaatacctgc atagtccacg aggcattttt
    58501 gaactgaagt atttcttttc cagtcacgtc aataccgagg gcggcggcga agcttcctcc
    58561 acggcgattc gtgcgctggt gaagaaatta atcgcggcgg aaaacccagc gaaaccgttg
    58621 agcgacagca agttaacctc tttgctgtcg gaacaaggta tcatggtggc acgccgcact
    58681 gttgcgaagt accgagagtc tttatccatt ccgccgtcaa accagcgtaa acaactcgtt
    58741 tgacccaacc gataaggaag acactatgca gctcaacatt accggaaata acgtcgagat
    58801 caccgaggca ctgcgcgaat ttgttacagc caaatttgcc aaacttgagc aatattttga
    58861 ccgaatcaac caggtctatg ttgttctgaa agtggagaaa gtcacccaca cctcagatgc
    58921 aacactgcat gtaaacggtg gtgaaattca tgccagcgcg gaaggtcagg acatgtacgc
    58981 tgccattgat ggcttaattg ataagctggc acgccagttg accaaacata aagataaact
    59041 gaaacaacac taattgtccg ggcaattagc atgtgcatgg cggcctgttg tgcggcacaa
    59101 cgggccattt gtacggttaa tgctccgagc ctgttccact gtttgagtgg gcaggttctt
    59161 aggtgaaatt atgacaaata atgatacaac tctacagctt agcagtgttc ttaacaggga
    59221 atgtacgcga agccgcgtcc actgtcagag caaaaaacgc gccctggaaa tcatcagcga
    59281 gctggcggcg aaacaactta gcctgccgcc tcaggtggtt tttgaagcta tcctgacgcg
    59341 ggaaaaaatg ggcagtaccg gtatcggcaa tggtattgca attccgcatg gcaaactgga
    59401 agaagatact ctgcgtgccg ttggcgtttt cgttcagctc gaaacgccta tagctttcga
    59461 tgccatcgac aaccaaccgg tggacttact ttttgccctg ctggtgccgg cagaccaaac
    59521 taaaacgcac ctgcatactc tgtcgctggt ggcgaaacgt ctggcggaca aaaccatctg
    59581 ccgccgttta cgcgcagccc agagcgatga agagctgtat caaatcatta cggataccga
    59641 aggtactccg gatgaagcgt agttattcgg taatgtctct tttagacgtt gtgaggagaa
    59701 acagtacatg gtactgatga tcgtcagcgg acgttcaggt tcaggtaaat ctgtcgccct
    59761 gcgtgcgctg gaagatatgg gtttttactg cgtggataac cttcccgtag tgttgttacc
    59821 cgatctggct cgaaccctgg ccgatcgcga gatttctgcc gccgtcagca ttgatgttcg
    59881 taatatgccg gagtcaccag aaatattcga acaggcgatg agtaacctgc ctgacgcttt
    59941 ctcaccgcaa ctactgttcc tggatgccga ccgtaatacc ttaattcgtc gttacagtga
    60001 cacgcgccga ctgcatccgc tttccagcaa aaacctgtcg ctggaaagtg ctatcgacaa
    60061 agaaagcgat ttgctggagc ctctgcgttc gcgagcggat ctgattgtcg atacctcaga
    60121 aatgtccgtt cacgaactgg ctgaaatgct gcgtacccgt ctgctgggta aacgtgaacg
    60181 cgaactgacc atggtctttg agtctttcgg cttcaaacac ggtatcccta tcgatgcaga
    60241 ttacgtcttt gacgtgcgct tcttgccgaa cccgcactgg gatccgaaac tgcgtccaat
    60301 gacaggtctt gataagcctg tcgccgcgtt cctcgaccgc cacacagaag tacacaattt
    60361 tatctaccag acgcgaagct atcttgagct atggttacct atgctggaaa ccaacaaccg
    60421 tagctacctg acggtcgcca ttggttgtac cggcgggaag caccgttcgg tgtatattgc
    60481 agagcaactg gcagactact tccgctcgcg cggtaaaaac gtccagtcac gccatcgtac
    60541 gctggaaaaa cgtaaaccat gaccgtcaag caaactgttg aaatcacaaa caagctgggc
    60601 atgcatgccc ggcctgcaat gaagctgttt gaattaatgc agggttttga cgctgaagtg
    60661 ctcttacgta atgacgaagg caccgaggct gaagccaaca gcgttattgc gctgctgatg
    60721 ttggattctg ccaaaggacg gcagattgaa gttgaagcga ccggtccaca ggaagaggaa
    60781 gcactggccg ccgttatcgc cctctttaat tctggttttg atgaagatta atcttcatca
    60841 ctttgacata caactccctt caaactcccc ccgacaataa gaaaatcacg tactgaaatc
    60901 gttctcaatc aacgtcattt gtacattttg tgcgcttttc actttcagaa gaaccttaag
    60961 aaaaccttaa gaggcattgt ttaggttttg tttaagttaa tcgaccatac tggagatcgt
    61021 cagaaaatat ttccaggaga tggcatgatt cgcttatctg aacaaagtcc cctgggcacc
    61081 ggaagacatc ggaaatgtta tgcgcatccg gaagatgccc aacgctgtat caagattgtc
    61141 taccatcgtg gcgatggtgg cgataaagag atccgccgtg agttaaagta ctacgcgcat
    61201 cttggtcgcc ggttaaaaga ctggagtgga ataccgcgct atcacggtac cgttgagacg
    61261 gattgcggaa ccggttatgt ctacgatgtg attgcagatt ttgacggcaa gccttccatt
    61321 acgctgaccg aatttgctga acaatgccgt tacgaagaag acattgcaca actgcgccag
    61381 ctactgaaac agctgaagcg ttatttgcag gataaccgta tcgtgacgat gtcgctgaag
    61441 ccgcagaata tcctctgtca ccgcatcagt gagtcagagg ttatcccggt cgtctgcgac
    61501 aacattggtg aaagcacatt gattccactg gcgacctggt cgaagtggtg ttgtctgcgt
    61561 aagcaagagc ggttatggaa acgatttatc gcccaaccgg cgctagctat tgccctgcag
    61621 aaagatctgc aacctcgtga gagcaaaacg ctggccctca cttcgcgcga agcttaatcc
    61681 agctggtgtt gttgcataaa cggctcaccg cctaactgat acatctgccg taaaatccac
    61741 gcctgacggc tacgcacgta gcccgatggc gaggagactt tgaaacgaag tggattaggt
    61801 aatacagctg ccagtaatgc agcttccgac cgggtaagtt tgctcgcggg tttgtggaaa
    61861 taacgttgtg ccgcagcttc gacgccaaac acaccgtcgc caaattcggc gatattcagg
    61921 taaaccgtca ggatacgctt tttgctccag accgtttcta tccccagcgt taatccggct
    61981 tccagccctt ttcgaaccca gctacgccca tcccataaaa agagattttt ggctgtctgt
    62041 tgagaaatcg ttgaagcacc gcgaatacgg ttttcattgc gctcgttgtg cgccagggct
    62101 ttctcaatgg aagcgacatc aaagccccag tgctcaggaa atttctgatc ttctgcggca
    62161 atcaccgcca gtcccatcca cggcgagatt tgatccatac tgacccagtc agaatgtgcc
    62221 acgtaacgaa aattgccatg cagccaggcg ctgacctgtc gctcgaccat taccgctgag
    62281 aagggaacag gcgcaacgct aaacaacgcg atgcccccgc cccagaaaac ggcgaggaca
    62341 accattaacc gcaaaagaaa acggcgaacg aaactaaaca ccgttaagcg gcttttactc
    62401 attcagccag aaccagcacg cgggaaacca gcttatcaat gccgctcgcc gcttctgcaa
    62461 tgttctgcgc cagcatatat gctggggtgg tgacaatctt attgtcttca tcaaccacga
    62521 tatcatcgac aggacacggc acatgctccg cgcccatctc ttccagcact tctgcggtat
    62581 cgatatcagt accgatggtc aaacgcagcg ggaaatcgaa aattttcggc agcatcgccg
    62641 gggcaataca cataaaacca agcggttttc cggcctgatg catcgcttgt gctagcgcct
    62701 ttaattcacg gtcaacggtg cattcgctac caagactggc aaaattgctt aaattcttcg
    62761 ccgcgccaaa cccccccggc acaatcaacg catccagttc agcggcatcg gcctgggcca
    62821 gaggacggat ttcaccgcgc gttattcgtg ccgcttcaat cagcacattg cgcgtttccg
    62881 tcatcgcttc gccagttaaa tggttgataa catcaacctg ctgcttatcc ggtgcaaagc
    62941 agaccgcctg cgcaccgctg cgtgaaatag ctaacaacgt caacaccgct tcatgaattt
    63001 cagaaccgtc atagacgccg catccgctca gaattacgcc aattttcttc attgtgatca
    63061 tcctttcgca attgactgaa acacattaat ttttttaata aaaatggtac gcatcacaca
    63121 tttaactgat tcatgtaaca aatcatttaa gttttgctat cttaactgcg tgcggcctga
    63181 aaaacagtgc tgtgcccttg taactcatca taataattta cggcgcagcc aagatttccc
    63241 tggtgttggc gcagtattcg cgcaccccgg tctagccggg gtcatttttt agtggctttt
    63301 gccacccacg ctttcagcac ttctacgtcg tgacgccact cttctttcat ctcttcaatc
    63361 cattcaccga cgttatcttc ccaggccgga aggtcaggag actgaatttg ctgacccagc
    63421 tgttgcagat ggcgtaaccc cactgacccc gccgcacctt taattttatg tccttcctca
    63481 acaatgcctt ttttatcctg cgccgtcaga ttcgactcca gcacgctgac atagcccggc
    63541 atcatcttct caaacaccgc taacccgtcg gtgatcagct tcggtcctac aagttcgaga
    63601 tactgttcca gcatgggaat atcgagcaat gcttctgatt tactgttctc ttctgtcgtc
    63661 accgtactct cctcatcatc ctgggtatcc cagaatttct tgatcatcgc ggttagcgcc
    63721 ggaacagaaa gcggcttact cagcacatca tccattccag cattgaggta ctcttgtttg
    63781 tctttcagca cgttagcggt taaggccacc agcggcggta aatcctcgcg cggataacgt
    63841 ttcgtcagtt cacgagagat atccagcccg gtcatatctg gcaactgaat atccagcaac
    63901 accaggtcgt attcgcccgg tttaaacatc tccagcgccg ccttgccggt catggcgaca
    63961 tcaacgctgt tacctaattt ttccagcaca gaacgcgcaa caatcacgtt cagttcaatg
    64021 tcttccacca gcagcacatt cagcgccggt aaaggcatat cgtcttcatc aaacgcatca
    64081 tcgacctctt ctgctaccga cggtgcgtgg atcgtcaacg taaaggttga acctttgccc
    64141 tgttcgctgg taaccgtaat atcgccgccc atatttttcg ccagacgacg agaaacggcc
    64201 agaccaatac cggtgccggt ggcaggttta ccgccatgac tgtctttcac ctggtaatac
    64261 atggcgaaaa ttttatccag ctcatcctgc ggaatgccga taccagagtc ttccacttca
    64321 aaatgcagca tatcgccttc atcgtagcgc acgcgcacgg taacctggcc ttgctgggtg
    64381 aatttgacgg cgttactgat gaggttccac aggatctgcc gtaaacgcgt cccgtcggta
    64441 atgacctgat gcggtaatgg cagcgtcggc tccaggttaa agcgcaatcc tttttgttgc
    64501 gcctgcaatg cggagagatt ttccagatcg gcaaggaagc tggtgaaatc aaccggttga
    64561 ttatcaagct ggaccttgcg ccgttccatc ttatccatgt cgataatatc gttaaagata
    64621 ttccccagcg tgacggccga aacatggatg gtcttgagat atttttcctg ctcggcggtg
    64681 agttcggtat ccagcagaat gcggctcaga ccgacgatac cgttcagcgg tgtacgcaat
    64741 tcgtgactga tggtggagat aaacgtcgtt ttgtcgcggc tggcccgttc aagcgcatcc
    64801 tgataccgct tacgctcggt aatgtcgcga ccaaagccca tcaaaccgtg acgtttaccc
    64861 acgcggtcgt agtacggcac tttacggatt tcaaagcagg ctttgcgccc gtccgggtaa
    64921 tccagccact gttcataggt cagtgacaca ttatgacgga acactttttc atcggtttca
    64981 atgacttttg cggcggcttc cggtgagtaa acatcagcag gtttcaggtg aaccagttgt
    65041 ttttcgcttt ttccggtcag cagctccatc gcgcggttac agccggaaaa ctctttatct
    65101 tcgttacgat aaaaaaccag gtcgggtgaa gcatcaagga aggaacgtaa gaatgaggat
    65161 tgctgctcga gctgaatttg tgtctcttcg cgctctttga tttcaatttt cagttggccg
    65221 aaggtttcct gcagttctgc ttccgctttt tcacgaacgg caatttcctg atttagctgg
    65281 gcaatattat cttttaactg aacgttgagg ctcaaatcgc gctcgcgcat ctcctccagt
    65341 ttttgcacca gccgtgacag acgttgtcgt gactcctcca gttgctcgac gaccaccgat
    65401 agaaagtaga ccgcccacgg cgtaatcagc aaaccaaaga agatagaacg aataacatca
    65461 atgctttcga cctgaccatg cagcaccatg gttaccgcca tttgtaccac aatggcaaga
    65521 acgacgaggg ccagcgccag caacattgag aagcgcacca gacctaactt catcatcagg
    65581 tcaacataat actgcgccag cagacgaatt tgcttcatta gggaattcct tcacgacaac
    65641 ctgccccaat aatacccaat tctgacagtt acgttataaa ttgtgtgaga aatgcggaat
    65701 tcccctcaac gacctactcc gttggaggta gccacggacg tcccagcgcc gatccctgta
    65761 cgccatgttc attcagatag cgatccagct cgaccattcc cgtccatcga ttttcgcacc
    65821 acagcggagc aagcagcgtc gggcgacggg cgctggcgga aatgcggtgg tagatcactt
    65881 ccggtggcgt atggcgaatc atttctccgg cagtgagcgt gtaatcctcc agttcaatac
    65941 cgttcaaacg ccccgcttcc caggctttcg ccataatgct gcctttcaca atatgcagcg
    66001 gatgcagctt tatgccgtcc acgccggttt caaccactcg ctccagcgtt tgcaaacatt
    66061 cagcctgccc ttcgccaggc aggccgacaa ttaagtggga acacaccttc agcccgcgct
    66121 gacgtgccag ctgggttgta cgctgataac aggcaaaatc atggccgcgg ttgatgcgat
    66181 gcagtgtttt gtcgtgggcg gtttgtagcc ccagctccag ccacacttcg taaccctggt
    66241 ccttatattc gcaaagcaga tccagcaccg catccggcac gcagtccggg cgggtaccca
    66301 cacacaaacc gacaatattg gcctggctca ccgcctgctg atacatagaa cgcagcacct
    66361 gaacttccgc aaaggtgctg gtatacgcct gaaagtaggc cagatagcgt ttagcgcggt
    66421 taactaaatt cgcctggtgc gccagttgct cggcaatgga acgatgctgc tgcgcttcat
    66481 cggcaaacga ggcaacatta cagaatgtgc agccaccacg cccgatggta ccgtcacggt
    66541 tagggcagct aaaaccgccg tgcagcgtca gcttatgcac cttttgccca taacgacggg
    66601 tgagatcacc accaaacata ttgactaatt tctgtaactg cataatctga tagaccgcgc
    66661 cttgaaaaga ggccaaagcc tgccattttt agctcaattc ggcgatgacc tggatcaatc
    66721 gtcccggcct gcttttatca actgcataat caatcaaaat taccgaaatt tcatgcataa
    66781 tcacataaat cacttttgct tatcttgtgt cagatttttt tatctcctga tggattttag
    66841 gcaaaaacag tagcatgaaa cgtcattacc aattaaggca gtataaaatg ctggttttgt
    66901 cgtcagttca aggcaggata agggttaaca cacctttatg acagtcagga attgactgtt
    66961 tctctaacga cttccctttt agccttaaag ataaaatcca ttttaatttc agtcatttaa
    67021 taaagaattt tgcgctaaag cacatttctg taccaataag cttgccattt gacctgtatc
    67081 agctttcccg ataagttgga aatccgctgg aagctttctg gatgagcagc ctgctcatca
    67141 tatttatgca gtaattgaga tcccctcttc accgtattaa ccgatgcgaa aaggacaaca
    67201 agggggcgaa tgcgaggcgc gcgtatgaca cgcaaacccc gtcgccacgc tctttctgtg
    67261 cccgtgcgca gcggttcgga agtggggttc ccgcagagcc tgggggaggt tcacgatatg
    67321 ttgtacgata aatcccttga gagggataac tgtggtttcg gcctgatcgc ccacatagaa
    67381 ggcgaaccta gccacaaggt agtgcgtact gcaatacacg cactggcccg catgcagcac
    67441 cgtggcgcga ttctcgccga tggtaaaacc ggcgacggtt gcggcttgct gttacaaaaa
    67501 ccggatcgct tttttcgcat cgttgcgcag gagcgcggct ggcgtttagc aaaaaactac
    67561 gctgtcggga tgctcttcct gaataaagat cctgaactcg ccgctgccgc acgccgcatc
    67621 gttgaagaag aactgcaacg cgaaaccttg tcgattgtgg gctggcgtga tgtccccact
    67681 aacgaaggcg tgctgggtga aatcgccctc tcctctctgc cacgcattga gcaaattttt
    67741 gtgaacgccc cggcaggctg gcgtccacgc gatatggagc gccgtctgtt tatcgcccgc
    67801 cgccgcattg aaaagcgtct cgaagccgac aaagacttct acgtctgtag cctgtcgaat
    67861 ctggtgaaca tctataaagg tctgtgtatg ccgacggatc tgccgcgctt ttatctggat
    67921 cttgcggacc tgcgtctgga atcggccatt tgcctgttcc accagcgctt ctccactaac
    67981 accgtaccgc gctggccgct ggcgcaaccg ttccgctatc tggcgcataa cggtgaaatc
    68041 aacaccatca ccggtaaccg ccaatgggcg cgtgcgcgta cttataaatt ccagacaccg
    68101 cttatccctg acctgcacga cgccgcaccg ttcgtcaacg aaaccggctc tgactccagt
    68161 tcgatggata acatgctgga actgctgctg gcaggcggga tggatatcat ccgcgccatg
    68221 cgtctattag taccacccgc ctggcagaac aacccggata tggacccgga actgcgtgcc
    68281 ttctttgact ttaactccat gcatatggag ccgtgggatg gcccggcggg catcgtgatg
    68341 tccgacggtc gttttgccgc ctgtaacctc gaccgtaacg gtctgcgtcc ggcgcgctac
    68401 gtcatcacca aagataagct catcacctgc gcctctgaag tcggtatctg ggattaccag
    68461 cctgacgaag tggtcgaaaa aggccgcgtc gggccaggcg aactgatggt tatcgacacc
    68521 cgcagtgggc gtattctgca ctcggcagaa accgatgacg atctgaaaag ccgccatcca
    68581 tataaagagt ggatggagaa aaacgtccgc cgactggtac cgtttgaaga tctgcccgat
    68641 gaagaagtgg gtagccgcga actggacgac gacacgcttg ccagctacca gaaacagttt
    68701 aactacagcg cggaagagct ggactccgta attcgcgtac tgggcgaaaa cggtcaggaa
    68761 gcggtcggtt cgatgggcga tgatacccca ttcgccgtgc tctccagtca gccgcgcatt
    68821 atttacgact acttccgcca gcagtttgcc caggtgacta acccgccaat cgacccgctg
    68881 cgtgaagcgc atgttatgtc gctcgccacc agtatcggtc gtgaaatgaa cgtcttttgc
    68941 gaagcagagg gccaggcgca ccgtttaagc tttaaatcgc cgattctgct ctactccgat
    69001 ttcaaacagc tcacgacgat gaaagaggag cactaccgcg cagatacgct ggatatcacc
    69061 tttgacgtca ctaaaaccac gctcgaagcg acagtcaaag agctgtgcga caaagccgaa
    69121 aaaatggtac gtagcggcac cgtgctgctg gtgctctccg accggaatat cgctaaagat
    69181 cgcctgccgg ttccagcccc gatggcggtt ggcgcgatcc agacccgtct ggtcgatcaa
    69241 agcctgcgtt gcgatgccaa catcatcgtc gaaaccgcca gcgcccgcga tccgcaccac
    69301 ttcgccgtgt tgctgggctt cggcgcgacg gctatttatc cataccttgc ctatgaaacg
    69361 ctgggccgcc tggtagacac ccatgcgatt gccaaagatt atcgtaccgt gatgctcaac
    69421 taccgtaacg gcatcaacaa aggcttgtac aaaatcatgt ccaaaatggg catctccacc
    69481 atcgcctctt accgctgctc gaaactgttt gaagcggtcg gtctacacga tgatgtagtg
    69541 ggcctgtgct tccagggggc ggtcagccgc attggtgggg caagctttga agacttccag
    69601 caggatctgc tgaatctgtc gaaacgtgcc tggctggcgc gtaagcccat cagccagggc
    69661 ggcctgctga aatacgtcca cggcggcgaa taccacgcct acaacccgga cgtggtgcgc
    69721 acgctgcaac aagcggtaca aagcggcgag tacagcgact atcaggaata cgcgaagctg
    69781 gttaatgagc gtccggcaac cacgctgcgc gatctgctgg caattacgcc gggtgaaaac
    69841 gcggtcaaca ttgctgatgt tgaaccggca agcgaactgt ttaaacgctt tgataccgcc
    69901 gcgatgtcta tcggcgcgtt aagcccggaa gcccacgagg cgctggcgga agcgatgaac
    69961 agcatcggcg gtaattcgaa ctccggtgaa ggcggcgaag acccggcgcg ctacggcacc
    70021 aacaaagtgt cgcgcatcaa gcaggtggct tccggtcgct ttggggttac tccggcgtat
    70081 ctggtcaatg ccgacgtcat tcagattaaa gtcgcccagg gcgcgaagcc aggcgaaggc
    70141 ggtcagttgc cgggtgataa agtcacgcct tacatcgcca aactgcgcta ttcggtgccc
    70201 ggagtgacgc tgatctcccc gccgccgcac cacgatatct actctatcga ggacttagcg
    70261 cagctcattt tcgacctcaa gcaggttaac ccgaaagcga tgatctccgt gaagctggtt
    70321 tccgaaccgg gagtaggcac catcgcgact ggcgtggcaa aagcttatgc ggacttgatc
    70381 accatcgcag gctatgacgg cggcaccggc gcaagtccgc tttcatcggt gaaatacgca
    70441 ggctgtccgt gggagctggg gcttgttgaa acccagcagg cgctggttgc taacggcttg
    70501 cgtcacaaga tccgtttgca ggtcgatggc ggcctgaaaa cgggtgtcga tatcatcaag
    70561 gcggcgattc tcggcgcaga aagcttcggc ttcggcactg gcccgatggt ggcgctcggc
    70621 tgtaaatatc tacgtatttg ccatctgaac aactgcgcaa cgggtgtagc aactcaggat
    70681 gacaaactgc gtaagaacca ctatcacggc ctgccattca aggtgacgaa ttactttgag
    70741 tttatcgccc gtgaaacccg cgagctgatg gcacagcttg gcgtaacacg tctggtggat
    70801 ctgattggtc gcaccgacct gctgaaagag ctggacggtt tcaccgccaa acagcagaag
    70861 ctggcgctgt cgaagctgct ggagactgcc gaaccgcatc caggtaaggc actctactgc
    70921 accgaaaaca acccgccgtt tgataacggc ctgctgaacg cgcagttgct gcaacaggcg
    70981 aaaccgtttg tcgatgagcg ccagagcaaa accttctggt tcgatattcg caacaccgac
    71041 cgttctgtcg gcgcgtcgct ttcaggctat atcgcccaga cgcacggcga tcaggggctg
    71101 gcagccgatc ctatcaaagc gtacttcaac ggcaccgcag gccagagctt cggcgtgtgg
    71161 aacgcgggcg gcgtggaact gtacctgacc ggtgatgcca acgactatgt cggtaaaggc
    71221 atggcgggcg gcttaatcgc cattcgtcct ccggttggtt ccgccttccg cagccatgaa
    71281 gcaagcatta tcggcaacac ctgcctgtat ggcgcgaccg gtggtcgtct gtatgccgca
    71341 ggccgcgcgg gtgaacgttt cggcgtgcgt aactccggtg ctatcaccgt ggtagaaggc
    71401 attggcgaca acggttgtga atatatgacg ggtggtatcg tctgcattct gggtaaaacc
    71461 ggcgttaact tcggtgcggg catgaccggc ggtttcgctt acgttctcga tgaaagcggc
    71521 gatttccgca aacgcgttaa cccggaactg gtcgaggtct taagcgttga cgctctggcg
    71581 atccatgaag agcatctgcg tggtcttatc accgagcatg tgcagcatac cggctctcag
    71641 cgcggtgaag agattctggc gaactggtca accttcgcca ctaaatttgc gctggttaaa
    71701 ccgaagtcca gtgatgtaaa agcactgctg ggtcaccgta gtcgtagcgc agctgagttg
    71761 cgcgtgcagg cgcagtaagg ggtagcaaca atgagtcaga atgtttatca atttatcgac
    71821 ctgcagcgcg ttgatccgcc aaagaaaccg ctgaagatcc gcaaaattga gtttgttgaa
    71881 atttacgagc cgttttccga aggccaggcc aaagcgcagg ctgaccgctg cctgtcgtgc
    71941 ggcaacccat actgcgagtg gaaatgcccg gtacacaact acatcccgaa ctggctgaag
    72001 ctcgccaacg aggggcgtat ttttgaagcg gcggaactgt cgcaccagac caacaccctg
    72061 ccggaagttt gcggacgagt ctgcccgcaa gaccgtctgt gcgaaggttc ctgcactctg
    72121 aacgatgagt ttggcgcggt gaccatcggc aacattgagc gctatatcaa cgataaagcg
    72181 ttcgagatgg gctggcgtcc ggatatgtct ggtgtgaaac agaccggtaa aaaagtggcg
    72241 attatcggcg caggcccggc aggtctggcg tgtgcggatg tcctgacgcg taacggcgta
    72301 aaagccgttg tcttcgaccg tcatccagaa attggcgggc tgctgacctt cggtattccg
    72361 gccttcaagc tggaaaaaga ggtaatgacg cgtcgccgtg aaatcttcac cggcatgggt
    72421 attgaattca aactcaatac cgaagtgggc cgcgacgtgc agctggacga tctgctgagt
    72481 gattacgatg ccgtcttcct tggcgtcggg acttatcagt caatgcacgg cgggctggaa
    72541 aacgaagacg ccgatggcgt gtacgcagcg ctgccgttcc ttatcgccaa caccaaacag
    72601 ttaatgggct ttggtgaaac ccgcgacgaa ccgttcgtca gcatggaagg caaacgcgtg
    72661 gtggtccttg gcggtggcga cactgcgatg gactgcgtgc gtacgtccgt gcgccaggga
    72721 gcgaagcacg ttacctgtgc ctatcgtcgt gatgaagaga acatgccggg ttcccgccgc
    72781 gaagtgaaaa acgcgcggga agaaggcgta gagttcaaat tcaacgtcca gccactgggg
    72841 attgaagtga acggtaacgg caaagtcagc ggcgtaaaaa tggtacgtac cgaaatgggc
    72901 gaaccggatg ccaaaggccg tcgccgcgcg gagatcgttg caggttccga acatatcgtt
    72961 ccggcagatg cggtgatcat ggcgtttggt ttccgtccac acaacatgga atggctggca
    73021 aaacacagcg tcgagctgga ttcacaaggc cgcatcatcg ccccggaagg cagcgacaac
    73081 gccttccaga ccagcaaccc gaaaatcttt gctggcggcg atatcgtccg tggttccgat
    73141 ctggtggtaa ccgctattgc cgaaggtcgt aaggcggcag acggtattat gaactggctg
    73201 gaagtttaag ctctgtccca gcgccgcctc ctcagaaaag agtcggcgct gaaattctcc
    73261 tttctttctg gaaagcgcct cgcataatct gaagaaaacc aatgtaatac gctcatctgg
    73321 attgttaccg gtagatgccc ggcgtatagc tgattccatt attcaacgca gcgccatata
    73381 aagggacaga taatggaatc gctctcagag agaacctcaa caggctacca gcaaatccat
    73441 gacggtatta ttcacctggt cgatagcgcc cggacggaaa cggtacgtag cgttaacgcg
    73501 ctgatgaccg cgacatactg ggaaattggc cgacgaattg tcgaatttga acaaggtggc
    73561 gaggccaggg ctgcgtatgg tgcgcagcta atcaagcgac tatcaaagga tttaagtcta
    73621 aggtataagc gtgggttctc tgcaaaaaac ttacgccaaa tgaggctttt ttacctcttt
    73681 tttcaacatg ttgaaattcg ccagacagtg tctggcgaat taacaccatt gggaattccg
    73741 cagacaccat ctgcagaatt cccatcggca aagatttggc agacgctgtc tgccaaattg
    73801 agaggctgtt tgatatttcc acagcttttg tattgcatct cctattttcg gtttgtatat
    73861 tcatgcaaat aaacgaagga aagttgccat gatttacatt gaacagcttg aattaatcca
    73921 taaaagtggt gatgtgttgt atccagtaaa aataaccaga aaatcatcag gaaaaactgc
    73981 cttccacctt gttccattcg gcttaaataa gactcacgac cttctagagg tggaagatgc
    74041 ttcagaagca attcggctgg tgattgatga acgtcattcc attcgctgca gtacacttac
    74101 agcaactatt acgaataaaa agggcaagcg aattaagcgg acaggtatat acagtatcaa
    74161 gggtgttaac ataaaagaat acaacgtcag gtaatggcac agagcggtcc gtcagttcag
    74221 gtttggctct gtgccaaaga tgggtcaggt cacacctgat ctaataaatt taagccatta
    74281 aatcagcctt aaccttcacc accacctttt taatctcacc cggctcgccg acaacgcatc
    74341 ctggtttatg cggttctccc ggcataaaca cggcgaacat tcccggtttt aagttgatgg
    74401 tttgctcgtt ttcaatggcg ctgcaaagct ggtaatcatc ttcatggtgg aactcttcac
    74461 attggcgcgc agtgcctgcc atgccaaaca gaatccgttc ctcaccgttt aataacagct
    74521 ggatatcaat gtattgctcg tgcaattccg cttttttctc gacgggcgat tgagtgttaa
    74581 acgtcatgac attcataaaa atattgtcgc cctgtaattc gtaacgaccc ggcgcttttt
    74641 cttgcggtct ggcagctaag gccagcgtta acgcgtcctg taacgcagga tgtaacccag
    74701 cagacggtaa tgactgtact tcacccatca tcataatttt tctccctggg ccaacagcgc
    74761 agccccaagt aaacctgcat catggcggta atgcgccgcc agtaaatcaa catgaaatgc
    74821 cgctggctcc tgcgccagat acatttccac caacgccaga tacccttctg ccagaccaac
    74881 gctgccacca accaccacgc actggcaatc agttgtggct ttaatatcag cgatcagcct
    74941 tgcaagcgta cgtgcggagc gatgaatcag ttgctgcgcc tgctcatcgc cctgcccggc
    75001 gcgtgtgaaa atagttctcg catccgcgcc agccagctcg ccctgcgctg ccgccgcaat
    75061 gccgcgacca gaagcaattg cttccacgca acctgtgcgt ccacagccgc agactgggcc
    75121 gtgtggatcg gcaagcatat gcccgatgtg ccccgccaga ccgccagggc cggtaagcag
    75181 tttgccgccg ctcactacac cgccgccgac gccagtggaa acggtgataa agaccatatc
    75241 ggttatatcg ccttccagcg cctgatactc tgcccatgct gcggcctgcg cgtcgttaat
    75301 ggcaatggtc ggcaaattgg taagttgttc cagcgttttg actaacggaa agtgtagcaa
    75361 tccaccgaga ttatgcggat tcagcgccag caaactgccg tcgcgaataa ttccggttga
    75421 agcgatggca acccgctgcg catgagcttg caacggagag actaatgcgg ataaggcatc
    75481 acgcaaggct tctggtgtct ggctggctgg cgtaggaagt tcacgacgat cgcggatctg
    75541 cccgtcagcg ccaatcagcg cggcggcaag tttagtaccg ccgatatcaa tcgccagtgt
    75601 ggtcatagca ccgccttttt catcgctgtg ttgtaccact gacaaatgtg ttcaagacgc
    75661 gtaatggcag aaccgaccgt caccgcccac gcgccgtggc gcatcgcatc tgccgcctga
    75721 gcaggcgtgt tgtaacgccc ttcggcaatc acccgacatc cggcttcgct caacgttttc
    75781 accagcgcca gatccggctc ttctggcgtt tcaggcgtgg tatagccaga aagcgtagtg
    75841 ccaataattt cggctcccag cttttggcat gccaggccgt cttccggcgt tgagcagtcg
    75901 gtcatcgcca gtaaaccgtg atggtgaata cgtgccagca gcgtttcaac aggcaccgga
    75961 cgcgggcggt cggtgccgtc aatggcgata atgtccgcgc ccgcctgcgc cagcgcatca
    76021 acatcttcaa tataggccgt gatgcgtacc ggagaatcct ccagatcgcg tttcacaatc
    76081 ccgataattg gcacgctcac caccgcacgc gtggcttgca gatttgccac accttcaatg
    76141 cgaatggcaa ccgcgcccgc ctgttctgcc gctaatgcca tggcggcgac gatttcgggt
    76201 ttatcgagcg ggctgtccgg aaccggctgg caggagacaa tcaggccacc gttagcagcg
    76261 attttttgat ccagttgtgc aagtaacgac atacatcttc ccttagcgaa aggcccggta
    76321 catagaccgg gcaacaggat taacttttgg ttttgactaa atcgtttttg gcgctgccaa
    76381 acggcacggc accgctgaat ggtttaccgt cgatagcgtc atgagtacgc aacgcttccg
    76441 ggcgcaacca acgctgaacg cgagaaggca tatccagccc aatcagcagg atcaccacga
    76501 acgtcagact gaacgagagc gatgccagcg cagtacccag atccagacgt tgagcgatca
    76561 acgcgccgat gattggggcc agtgcaccgc ccaatgcgcc aacgttgtag gtaaagccca
    76621 ggcccgctgc acgctggtcg gtatcgaaat aaccgccaat cagttttggt aagatcccgg
    76681 cgatcccttg tccaagcatt tgctggaaga acagtaacag accgagcacc cagacgtttg
    76741 cgccgccaat cgcaaatacc ggaataatca gcagctgcga ggccagcagg ctacaaacgt
    76801 acgctttgcg ggttcccagc cagtcaccga ggaagccacc tacgcagcat cccaccgccg
    76861 cgccaaagcc actaaagaac agcacattgg ctacagtatg cgggttataa gccagatcgg
    76921 ttttcagata cgttggcagc agcgcctgaa tcggccatga gtagaggaaa gcaaacaaca
    76981 cgaccaccat cagcattacg cccgttggcc agcgtttgcc tgtactctgc accataaagc
    77041 tgataaagat tgcggcgcat aacagcccaa gaacagcgac gatcgcggca ttttgcaggt
    77101 taccggcgaa gcagaaccac agcgcagtag ccgccgccag tgtcattacg atattggcaa
    77161 tacgatgttc accacggtag agaatatcca ccattgtgcg tactggtgct ttacctgcgt
    77221 gtttctcttt ccagtcttcc gcttccggga tgtttttacg cagccagaga gcaaagatga
    77281 ttggcaaaat gccgataaag aacagcgcac gccagcccca gaccggaacc accaggctat
    77341 agacctgagc ggcaacgacg gcccccacag agaagcctga aatcaaaaaa ccactggctt
    77401 tgttacgcag atgttttggc cagctttcaa tgacataggt ggcgctggaa ccgtattcac
    77461 ccgccatccc catgccgatg accagacgag cgataaacat ggtgatgtag cctggcgcaa
    77521 agccgcaggc cagcgtcccg gccgagaaga gaacgatgct ggtgaccatt gccagacgac
    77581 gcccgtagcg gtcacccata gcgccgagca tcaggccgcc gaaccagcga gagataaagg
    77641 ctgcagagat cagacttgcc gcctgcaccg tcgtcagccc gaattcacct tgtacttcgg
    77701 tgagtaccag ggcgattaaa acgaaatcaa aaccgtcaag cagatatccc aaccaggcag
    77761 cggaaaatgc gcgccattgt gcacggttga gatggcgata ccacgggata ttctgggttg
    77821 tagtactcat tgtgagtctc ccgcggtggg cgatgcccac acgctttggt atgaaaattg
    77881 tagggtacag atgcgtttat ttcccctcac ccggtagggg cgagcgaggg gaaacaactc
    77941 acccgcgctc ttgcatcaac tgctgggcca gcgccttcag ttctggcaga tatttttcat
    78001 ctaccggtcc aaacggtttg cggcacagcg gcacagaaac gacatccata taatggagga
    78061 cagttttcag gccgcggaat acgcccgttt tgatcagtaa atcaatgact ttattgcatt
    78121 cagtttgcag tttctgcgcg gtctggatat cgccttcttt cagcgcctta acgatcccct
    78181 gatagcgcca gcccatgatg ttgtaggtac tgccgatacc accatcagcg cccgccagca
    78241 gaccagaggc gaagatttcg tcgtaaccgt tatagagcac aagatcagga tgttcacgac
    78301 ggatctgctc catctgatag agatcgccag aggtctgttt cagcgcacct acgccaggca
    78361 atgtaacaag tgtgttgatc tgatccaggg tcagttttac cccactcagg gctggaatgt
    78421 tgtacaccac catcggcaaa ccatccgccg aatcaataat tgcccgatag tgatcgcagt
    78481 gttcttcaaa gctgaaagga tagtagaacg gcgtgacggc ggagacggca tcgaagccat
    78541 aacgtttagc cgatgccgca agttgttggc tttcggcggt gctgacgcaa ccgacgtggg
    78601 cgatgagttt aatcttacct ttcgcctctt cggcgacgat ttccagtacc tgttcacgct
    78661 cggaaaggct ttgtacaaag gcctcgccgg tcgaaccacc cacgtataaa ccgtcgatgc
    78721 cctgctgaat attgaactga accaggcgac gcagactcgc tttatccagt gcttgttgtt
    78781 ggtcaaaagg agtcaggagt gcagccatta cgccacgtaa attcgttgcc ataaatacct
    78841 ctgaagtgat gcttgtctga taaacgatat acctttatac ctgttatacc agatcaatta
    78901 agcaacaccc catacagaaa gcttataatg cgatctgctt cactaaagtg gcattatttc
    78961 tttttgttgg tggtctgacc gaaagcgtgc caggtagcag agacgctgtt gagatgcgat
    79021 tgcaacgcac gatcggcttc gtcaggatca tgacggcgga tcgcatcaac gatcgcaata
    79081 tgctgttgat aactaacgtt gttatgttcg tgcagtgcct gatcggtaac cgttgggcgt
    79141 gcggcaataa gccagtcgag cagggcaacg tggatcgcca tgaagattgg gttaccgggg
    79201 atctccgcca gcacgcggtg gaaatcaacg tctgaacgaa tgaatgccgc gttgttatcc
    79261 agcgactgac tgttgatttc cagtgctttt gccagcaaat cgatttgctc atcggtggca
    79321 tgttcagccg catagcgcac cagactggat tcaaagaaca gacgtaattg ttcgaaatgg
    79381 gcaatcccac cgggatgaga aaggaaatct ttcgccatgc cggaaagctc accgatgata
    79441 gtgtccgcag aaggacgcga gacgcgagcg cgttcgccgt tgtttatttg caccagacct
    79501 ttgcgtttta acgctgccag cgcttcacgc accgaaggac gcccgacgtt aaagaacgcc
    79561 atcagttcgc gttcagacgg taattgttca ccttcgccaa attcacgacg gcggatcatc
    79621 tgttccagct cttcttccac catttcggag agttttttac gcgccagcgg gcggctacgc
    79681 aagttgcgac caattgcagg tgaagaatct tcggtttgcg aatcaaatgc gttcataagg
    79741 cccattctgt aaggtcagtg tgattaacat catcagtgac atcctatcac aggattgaaa
    79801 gtaggggaaa atggcagggt tttctctttg tgcctcatca ttaccataat taacggaata
    79861 attaactat
//
LOCUS       DAPHBJ010000003        73591 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.003, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000003 DAPHBJ010000000
VERSION     DAPHBJ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 73591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.003"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(13..870)
                     /gene="murI"
                     /locus_tag="RHZ76_000154"
     CDS             complement(13..870)
                     /gene="murI"
                     /locus_tag="RHZ76_000154"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDV2810859.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPGAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(815..2659)
                     /gene="btuB"
                     /locus_tag="RHZ76_000155"
     CDS             complement(815..2659)
                     /gene="btuB"
                     /locus_tag="RHZ76_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HDV2810860.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     regulatory      complement(2724..2914)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3028..4128
                     /gene="trmA"
                     /locus_tag="RHZ76_000156"
     CDS             3028..4128
                     /gene="trmA"
                     /locus_tag="RHZ76_000156"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HDV2810861.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4168..4527)
                     /locus_tag="RHZ76_000157"
     CDS             complement(4168..4527)
                     /locus_tag="RHZ76_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HDV2810862.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4527..5177)
                     /gene="fabR"
                     /locus_tag="RHZ76_000158"
     CDS             complement(4527..5177)
                     /gene="fabR"
                     /locus_tag="RHZ76_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HDV2810863.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5508..6908
                     /gene="sthA"
                     /locus_tag="RHZ76_000159"
     CDS             5508..6908
                     /gene="sthA"
                     /locus_tag="RHZ76_000159"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HDV2810864.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFIIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(6891..7808)
                     /gene="oxyR"
                     /locus_tag="RHZ76_000160"
     CDS             complement(6891..7808)
                     /gene="oxyR"
                     /locus_tag="RHZ76_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HDV2810865.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(8075..9448)
                     /gene="argH"
                     /locus_tag="RHZ76_000161"
     CDS             complement(8075..9448)
                     /gene="argH"
                     /locus_tag="RHZ76_000161"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDV2810866.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(9509..10285)
                     /gene="argB"
                     /locus_tag="RHZ76_000162"
     CDS             complement(9509..10285)
                     /gene="argB"
                     /locus_tag="RHZ76_000162"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDV2810867.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(10293..11297)
                     /gene="argC"
                     /locus_tag="RHZ76_000163"
     CDS             complement(10293..11297)
                     /gene="argC"
                     /locus_tag="RHZ76_000163"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDV2810868.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            11451..12602
                     /gene="argE"
                     /locus_tag="RHZ76_000164"
     CDS             11451..12602
                     /gene="argE"
                     /locus_tag="RHZ76_000164"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HDV2810869.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            12954..15605
                     /gene="ppc"
                     /locus_tag="RHZ76_000165"
     CDS             12954..15605
                     /gene="ppc"
                     /locus_tag="RHZ76_000165"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HDV2810870.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPIRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            15787..17520
                     /locus_tag="RHZ76_000166"
     CDS             15787..17520
                     /locus_tag="RHZ76_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HDV2810871.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            17735..18586
                     /locus_tag="RHZ76_000167"
     CDS             17735..18586
                     /locus_tag="RHZ76_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2810872.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFRLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(18573..18914)
                     /locus_tag="RHZ76_000168"
     CDS             complement(18573..18914)
                     /locus_tag="RHZ76_000168"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDV2810873.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(18916..19794)
                     /locus_tag="RHZ76_000169"
     CDS             complement(18916..19794)
                     /locus_tag="RHZ76_000169"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HDV2810874.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(19760..22057)
                     /locus_tag="RHZ76_000170"
     CDS             complement(19760..22057)
                     /locus_tag="RHZ76_000170"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDV2810875.1"
                     /translation="MTNRISRLKTALFSNTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQTSLTQGEDAAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(22108..22428)
                     /locus_tag="RHZ76_000171"
     CDS             complement(22108..22428)
                     /locus_tag="RHZ76_000171"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDV2810876.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(22443..23522)
                     /locus_tag="RHZ76_000172"
     CDS             complement(22443..23522)
                     /locus_tag="RHZ76_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDV2810877.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICVPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            23831..26332
                     /gene="ptsP"
                     /locus_tag="RHZ76_000173"
     CDS             23831..26332
                     /gene="ptsP"
                     /locus_tag="RHZ76_000173"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDV2810878.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVYSGSAGGILTPISSLDLNALSNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            26344..27006
                     /gene="fsa"
                     /locus_tag="RHZ76_000174"
     CDS             26344..27006
                     /gene="fsa"
                     /locus_tag="RHZ76_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDV2810879.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGVVVKIPVTSEGLAAIKLLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQTLLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            27017..28120
                     /gene="gldA"
                     /locus_tag="RHZ76_000175"
     CDS             27017..28120
                     /gene="gldA"
                     /locus_tag="RHZ76_000175"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HDV2810880.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            28395..29012
                     /locus_tag="RHZ76_000176"
     CDS             28395..29012
                     /locus_tag="RHZ76_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HDV2810881.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGNTLFYDPAYVRLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFSRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFNWRMVGHYRYFVK"
     gene            complement(29039..29944)
                     /gene="yijE"
                     /locus_tag="RHZ76_000177"
     CDS             complement(29039..29944)
                     /gene="yijE"
                     /locus_tag="RHZ76_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HDV2810882.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(30037..32217)
                     /gene="katG"
                     /locus_tag="RHZ76_000178"
     CDS             complement(30037..32217)
                     /gene="katG"
                     /locus_tag="RHZ76_000178"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HDV2810883.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKFTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(32546..33436)
                     /gene="metF"
                     /locus_tag="RHZ76_000179"
     CDS             complement(32546..33436)
                     /gene="metF"
                     /locus_tag="RHZ76_000179"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDV2810884.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(33785..36217)
                     /gene="metL"
                     /locus_tag="RHZ76_000180"
     CDS             complement(33785..36217)
                     /gene="metL"
                     /locus_tag="RHZ76_000180"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HDV2810885.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(36214..37380)
                     /gene="metB"
                     /locus_tag="RHZ76_000181"
     CDS             complement(36214..37380)
                     /gene="metB"
                     /locus_tag="RHZ76_000181"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDV2810886.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKGSK"
     gene            37657..37974
                     /gene="metJ"
                     /locus_tag="RHZ76_000182"
     CDS             37657..37974
                     /gene="metJ"
                     /locus_tag="RHZ76_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HDV2810887.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            38158..38766
                     /locus_tag="RHZ76_000183"
     CDS             38158..38766
                     /locus_tag="RHZ76_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HDV2810888.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            38773..39396
                     /locus_tag="RHZ76_000184"
     CDS             38773..39396
                     /locus_tag="RHZ76_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02117 family protein"
                     /protein_id="HDV2810889.1"
                     /translation="MKKIIPAVVCLLLCSCSTFPQAVAPVNNDFAGQHNIYIVSHGWH
                     TGIVVPAAIVNRVLPQLDARFAQPKWYEIGWGDKGFYQAQEITSRLTLQAMFWSTGAV
                     MHVVAFSAPPERYFPGSEVKPLTINNGQLATLMLFIGRSFSRSEAGNVIPLKKGIYGD
                     SQFYAANGRYGILNTCNKWTAKGLQSAGIEIEPALKLTAGSVISAMP"
     gene            complement(39451..39663)
                     /gene="rpmE"
                     /locus_tag="RHZ76_000185"
     CDS             complement(39451..39663)
                     /gene="rpmE"
                     /locus_tag="RHZ76_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDV2810890.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            39866..42064
                     /gene="priA"
                     /locus_tag="RHZ76_000186"
     CDS             39866..42064
                     /gene="priA"
                     /locus_tag="RHZ76_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDV2810891.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQHHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADEKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            42220..43245
                     /gene="cytR"
                     /locus_tag="RHZ76_000187"
     CDS             42220..43245
                     /gene="cytR"
                     /locus_tag="RHZ76_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HDV2810892.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            43337..44296
                     /gene="ftsN"
                     /locus_tag="RHZ76_000188"
     CDS             43337..44296
                     /gene="ftsN"
                     /locus_tag="RHZ76_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HDV2810893.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASSQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            44389..44919
                     /gene="hslV"
                     /locus_tag="RHZ76_000189"
     CDS             44389..44919
                     /gene="hslV"
                     /locus_tag="RHZ76_000189"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDV2810894.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            44929..46260
                     /gene="hslU"
                     /locus_tag="RHZ76_000190"
     CDS             44929..46260
                     /gene="hslU"
                     /locus_tag="RHZ76_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HDV2810895.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            46327..47253
                     /gene="menA"
                     /locus_tag="RHZ76_000191"
     CDS             46327..47253
                     /gene="menA"
                     /locus_tag="RHZ76_000191"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDV2810896.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            47346..47831
                     /gene="rraA"
                     /locus_tag="RHZ76_000192"
     CDS             47346..47831
                     /gene="rraA"
                     /locus_tag="RHZ76_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HDV2810897.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(47916..48161)
                     /gene="zapB"
                     /locus_tag="RHZ76_000193"
     CDS             complement(47916..48161)
                     /gene="zapB"
                     /locus_tag="RHZ76_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HDV2810898.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            48586..49431
                     /gene="glpF"
                     /locus_tag="RHZ76_000194"
     CDS             48586..49431
                     /gene="glpF"
                     /locus_tag="RHZ76_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HDV2810899.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            49454..50962
                     /gene="glpK"
                     /locus_tag="RHZ76_000195"
     CDS             49454..50962
                     /gene="glpK"
                     /locus_tag="RHZ76_000195"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDV2810900.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            51097..52107
                     /gene="glpX"
                     /locus_tag="RHZ76_000196"
     CDS             51097..52107
                     /gene="glpX"
                     /locus_tag="RHZ76_000196"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDV2810901.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            52204..52950
                     /gene="fpr"
                     /locus_tag="RHZ76_000197"
     CDS             52204..52950
                     /gene="fpr"
                     /locus_tag="RHZ76_000197"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HDV2810902.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPHCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESTIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(52955..53383)
                     /gene="uspD"
                     /locus_tag="RHZ76_000198"
     CDS             complement(52955..53383)
                     /gene="uspD"
                     /locus_tag="RHZ76_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HDV2810903.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(53410..53709)
                     /locus_tag="RHZ76_000199"
     CDS             complement(53410..53709)
                     /locus_tag="RHZ76_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HDV2810904.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAATSSADEGGSVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(53921..54361)
                     /locus_tag="RHZ76_000200"
     CDS             complement(53921..54361)
                     /locus_tag="RHZ76_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDV2810905.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            54462..55061
                     /locus_tag="RHZ76_000201"
     CDS             54462..55061
                     /locus_tag="RHZ76_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HDV2810906.1"
                     /translation="MKPGCTLFFLLCSALTVTTAAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            55169..55936
                     /gene="tpiA"
                     /locus_tag="RHZ76_000202"
     CDS             55169..55936
                     /gene="tpiA"
                     /locus_tag="RHZ76_000202"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDV2810907.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(55991..56746)
                     /gene="cdh"
                     /locus_tag="RHZ76_000203"
     CDS             complement(55991..56746)
                     /gene="cdh"
                     /locus_tag="RHZ76_000203"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HDV2810908.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFIMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(56853..57842)
                     /gene="sbp"
                     /locus_tag="RHZ76_000204"
     CDS             complement(56853..57842)
                     /gene="sbp"
                     /locus_tag="RHZ76_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HDV2810909.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(58162..59124)
                     /gene="pfkA"
                     /locus_tag="RHZ76_000205"
     CDS             complement(58162..59124)
                     /gene="pfkA"
                     /locus_tag="RHZ76_000205"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDV2810910.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(59305..60207)
                     /gene="fieF"
                     /locus_tag="RHZ76_000206"
     CDS             complement(59305..60207)
                     /gene="fieF"
                     /locus_tag="RHZ76_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HDV2810911.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(60356..60856)
                     /gene="cpxP"
                     /locus_tag="RHZ76_000207"
     CDS             complement(60356..60856)
                     /gene="cpxP"
                     /locus_tag="RHZ76_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HDV2810912.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            61006..61704
                     /gene="cpxR"
                     /locus_tag="RHZ76_000208"
     CDS             61006..61704
                     /gene="cpxR"
                     /locus_tag="RHZ76_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HDV2810913.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            61701..63074
                     /gene="cpxA"
                     /locus_tag="RHZ76_000209"
     CDS             61701..63074
                     /gene="cpxA"
                     /locus_tag="RHZ76_000209"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HDV2810914.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(63180..63854)
                     /gene="yiiM"
                     /locus_tag="RHZ76_000210"
     CDS             complement(63180..63854)
                     /gene="yiiM"
                     /locus_tag="RHZ76_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HDV2810915.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(64003..64986)
                     /gene="kdgT"
                     /locus_tag="RHZ76_000211"
     CDS             complement(64003..64986)
                     /gene="kdgT"
                     /locus_tag="RHZ76_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HDV2810916.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(65246..65866)
                     /gene="sodA"
                     /locus_tag="RHZ76_000212"
     CDS             complement(65246..65866)
                     /gene="sodA"
                     /locus_tag="RHZ76_000212"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HDV2810917.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPIMGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            66151..67185
                     /gene="rhaT"
                     /locus_tag="RHZ76_000213"
     CDS             66151..67185
                     /gene="rhaT"
                     /locus_tag="RHZ76_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HDV2810918.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLISTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKSLIIHNVLLSTLGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(67182..68048)
                     /gene="rhaR"
                     /locus_tag="RHZ76_000214"
     CDS             complement(67182..68048)
                     /gene="rhaR"
                     /locus_tag="RHZ76_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HDV2810919.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(68104..68940)
                     /gene="rhaS"
                     /locus_tag="RHZ76_000215"
     CDS             complement(68104..68940)
                     /gene="rhaS"
                     /locus_tag="RHZ76_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HDV2810920.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            69228..70697
                     /gene="rhaB"
                     /locus_tag="RHZ76_000216"
     CDS             69228..70697
                     /gene="rhaB"
                     /locus_tag="RHZ76_000216"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDV2810921.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQEQQINDLPALISATQALPACRFIINP
                     NDDRFINPETMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            70694..71953
                     /gene="rhaA"
                     /locus_tag="RHZ76_000217"
     CDS             70694..71953
                     /gene="rhaA"
                     /locus_tag="RHZ76_000217"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDV2810922.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTAGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSRRG"
     gene            72404..73228
                     /gene="rhaD"
                     /locus_tag="RHZ76_000218"
     CDS             72404..73228
                     /gene="rhaD"
                     /locus_tag="RHZ76_000218"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDV2810923.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            73238..73552
                     /gene="rhaM"
                     /locus_tag="RHZ76_000219"
     CDS             73238..73552
                     /gene="rhaM"
                     /locus_tag="RHZ76_000219"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDV2810924.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT        17957 a        19799 c        18829 g        17006 t
ORIGIN      
        1 cattcaacca aatcagccta aaactgccag tttttcgagc gtttcgaagc cgtaacgctg
       61 taaaacgggc aataattgtt cagctcctgg cgtcattgcc atacaaaagg caatattcgc
      121 atcggcagat tttgcatccg gggcttcatg ttctaacaac caggccgttc ggcgagcaat
      181 cgctgcgcca gaatccacca gccgggttcc ctctggcagc acttgtaaca gttcttcttg
      241 tagtagaggg aaatgggtgc aacccaatac aacggtatct ggcggctctt tcattcttaa
      301 ccacgggcgt aggatacgtt ttagtgcatc cagagaaaca tcttcgccat gtagcttcgc
      361 ttcagccaac tcaaccatct ctgccgagcc cagcatttct atctggcatt cattagcgaa
      421 acgcgcgatc agctcatgag tataagaacg tttaactgtt ccgcgggttg ccagtaatcc
      481 gacaatgcca tttgccgtca gacgtgcagc aggtttaatc gccggcacga caccaacaac
      541 cgggaagtcg aacttttcgc gtaatgcagg aagtgaaacg gtactggcag tgttgcaagc
      601 gaccacagcc agcgcaaggg gataacgttc ttgcaccgcg gtgacaattg ccaccactcg
      661 ctcaacaata aacgcttcgc ttttttcgcc atacgggaaa gcgacgttat cgaaagcata
      721 aatgtaatgg agatccggta agagatgccg gatctcgtca tagaccgaca acccaccgac
      781 gccggagtca aacaccagca cggtgggacg tggttcagaa ggtgtagctg ccagacaagg
      841 tgtattcccg tcctgcagtt tggtagccat agactgtctc ataatctttg tcgaacaggt
      901 tggctatttt accacgaact gtcaggtgag aggtgaccgg atacgcaacc gcaagatccc
      961 acaagctcac accgcccatt ttaacggttt gataaggata agatgagtaa tccttatcat
     1021 agcgagtgcc taaatactga taagtaatac cccagtcgaa gtcatacaac tgccagtcga
     1081 gctggtattt cacctgctgt ttagcacggc gtaacaacgg cgtgtcggta atcgcattgc
     1141 gcgcatcgac ataatcataa ctcacagtat gcgtcagtgg tccggtatca aaattggcgg
     1201 tcgcctcgac gcccttaatc cgcgctttcc cttcgttgta atatttcagg gtgtgatcat
     1261 cataatcgat caagtcactg acatcgttac gatatccgga aatacgccag ttcaccccag
     1321 cggttaagcc ttcaaacgcg ccttcccact gtttgctttt ctccgggtcc agattcggat
     1381 ttccgtagaa gccatacagt tgccccagat ttggtgcctt ataagatgtc ccgtaggaag
     1441 caatgaagcg ataaccttcg atgaattccc aaccggcgct ggtttgccag gttccatgac
     1501 gaccaaactg tgagttatcg tcgctgcgtg ctgcgccttc aaaggtaaaa tcgccgactt
     1561 gttgcagccc ggtcagatag atgccggtat tacgttgatc atatccatcc tcaacataac
     1621 ctgtacccgg cgtcgtagtc tgtttctgcc agtcgacacc cgcaccaata ctaccgtgac
     1681 caacgatgac attgtttgcc cactggacgg tgtattgctt catctcatcg agcgtcgccg
     1741 acgaatcata acgaccataa tggggatcgt agttgtaatc tttgctatgg ctatagctgg
     1801 taatgagttg tgatttaatc agttcgccgt tatagcgcag cccggcgtcc caactttggc
     1861 tatagagttt acgggtatcg agcaacggtg aaccgggaga ataatacgcg tcataattgg
     1921 tacggttatc atagccatag ccgcgcacaa agccgctcca ggcatcagta aagttatgct
     1981 ccagcgcgcc ataaagcgtt ttacttaaaa aaccatcgtt atctgtctgc gcttgcgttc
     2041 cggtattacc ataggcaaca acatcataac catgagtatg ggcataatcg cccaacagcg
     2101 ttacccgtgt cttatccccc agttgttgct gcgtagagac atcatagttc tgataactat
     2161 tgcttcccca ccctgctgaa atttccgttc cgggttcatc gcgcgtcgtg atgatattca
     2221 ccaccccgcc tattgcatcg gaaccataaa cagcggagcg cggcccacgg atatattcaa
     2281 cacgctggac aagcgcaata gggaactggc taaggtcggc agaaccactc acccccgcca
     2341 gattcaggcg tacgccatca attaacacca acacatgact ggcatttgta ccgcgaataa
     2401 aaatagatga gagctgacct gaaccgccgt tttgggtgat atcgacgccc ggaagacggc
     2461 gcagcacatc attgaccgag gtcgactgcc agcggtcgat atcctgacgg gtcacaacgg
     2521 tggttggtgc aagcacagtg ctgcgcggct gttcaaaacg gttagcagta acgacgagag
     2581 tatccgggct ggtatcctgt gcccaagcgg aaaatgccgt gacggaacac gccgtcagca
     2641 gcgaagcttt tttaatcatt gtaaagcatc cacaatagaa gaaggatgcc gcaggtttca
     2701 tcaatattac gcgatgatga gaaccagatg cgacgttggc cggcaggtct tcgggcttgg
     2761 aggggtatct aagatactaa gagatgatga cttcccaccg gatggcagtg tccgcataac
     2821 gcaatcatcg cacctttcct taccgctgcg cgtcagctcc agattcgcac tggattccct
     2881 attaactcac aggaccggca agtggatgct acaggttgta acaagttact gtccagacgt
     2941 agctcacaaa taggaattca tcaagatctg gacatctgat gagcaatccc tacaatcgcc
     3001 gcgtacttta atttttcagg atacatcatg acccccgaac accttccaac agaacagtat
     3061 gaagcgcagt tagccgaaaa agtggtacgt ttgcaaagta tgatggcacc gttttctgac
     3121 ctggttccgg aagtgtttcg ctcgccggtc agtcattacc ggatgcgcgc ggagttccgc
     3181 atctggcacg atggcgatga cctgtatcac atcattttcg atcaacaaac caaaagccgc
     3241 atccgcgtgg atagcttccc cgccgccagt gaacttatca accagttgat gacggcgatg
     3301 attgcgggtg tgcgtaataa tcccgttctg cgccacaagt tgttccagat tgattacctc
     3361 actacgctga gtaatcaggc ggtggtttcc ctgctatacc ataagaagct ggatgatgag
     3421 tggcgtcagg aagcggaggc cctgcgcgat gcactgcgcg cgcagaatct gaatgtgcat
     3481 ctgattggtc gggcaacgaa aaccaaaatc gagctggatc aggattacat cgatgaacgt
     3541 ctgccggtcg cagggaaaga gatgatctac cgtcaggtag aaaacagctt tacccagccg
     3601 aacgcggcga tgaatattca gatgctggaa tgggcgctgg acgtaaccaa aggctcaaaa
     3661 ggcgatttac tggagctgta ctgcggcaac ggtaactttt cattagcgct ggcgcgcaat
     3721 tttgatcggg tattagccac cgaaatcgct aagccgtcgg ttgctgctgc gcaatacaac
     3781 atcgcagcta accatattga taacgtacaa attattcgta tggcggcaga agaatttact
     3841 caggcgatga atggtgtgcg cgagtttaac cgcctgcaag ggatcgactt aaagagttat
     3901 cagtgcgaaa ccatttttgt cgacccaccg cgcagcggtc tggacagtga aaccgagaaa
     3961 atggtgcagg cgtatccgcg tattttgtac atctcctgta acccggaaac gttatgcaag
     4021 aatctggaaa cattaagcca gacgcacaag gtcgaacgtc tggctctgtt tgatcagttc
     4081 ccctacacgc accatatgga gtgcggcgta ttactgaccg cgaagtaaaa cccatgccgg
     4141 atgcgccagc atccggcata ataccgatta ctcagcaact tcctgcttac ggttacgcat
     4201 cttcgcgcca atccagaaca ccataatgac ggagagtact gccgggaaga agttagagcc
     4261 gatatccgga tattccgcac gaactaccgt gctgtacagc agtacgccga gaataaaaca
     4321 ggcagctgcc agacccggca agcctaccgg catagtgcga ttaagataac gttgatgcag
     4381 gcagtaaacc gtcagcacca gggaaataat cgggaataca gaaaatggca caatggagct
     4441 aaacagcgct gcgaaagtac cattaatcga taagccagca actaacgcca gcagcagcgt
     4501 acctctatct tgatttgctt gtttcattac tcgtccttca catttcccgg aataattgcg
     4561 gttttctctt gttcacggcg ataccagtaa taagcccctt tcgaaatcat tcgcagttgc
     4621 agtaccagtc gctcttctaa ttgccgacgt tgttcgacgc cgacgtccaa cgcctcggca
     4681 cccgcactga agacaattgt caccattgct tcggcttgcg cttcagtaaa cgcacgcggc
     4741 atatggtttt cgagttccag atagtccgca agttccgcaa tgaagtgctg aatttcacgc
     4801 gcaacggcgg cacgaaacgc agcggaggtg ccggagcgtt cccgcaataa taaccggaag
     4861 gcgttaggat tattaccgat gaactccata aatgtggaga ccgaggtgcg gatcacactc
     4921 ccgcctttgg cgatacgctg acgcgcctgg cgcatgagtt ggcgtagcat taaaccgctc
     4981 tcatcaacca tggtcagacc cagttcgtct acgtcgcgga aatgccgata aaaagaggtg
     5041 ggagcaatgc ccgcttcacg cgccacttca cgcaaactca ggctggcgaa gctgcgttca
     5101 gcacttaatt ggctaaatgc ggcttccacc agcgaacggc gggttttttc tttttgttgc
     5161 gctcttacgc ccatcacgat gtctgaatcc ttgccaaaag tactgctggc actataccag
     5221 agaatgaaca tactggattg ctccagtatt gtgaatgaac ggtaacgcaa taaaacatat
     5281 ttactgcaat tctttggccg gttcttttac gtacagcgga aacctgccgc ttaaacggag
     5341 agtatcgtcg ataaaaatcc aataaaacgt cagggcaaaa gtaagaaaca gacaaagcaa
     5401 aggccgctca ggatatagcc agataaatga cggggatcaa ttggcttacc cgcgataaaa
     5461 tgttaccatt ctgttgcttt tatgtataag aacaggtaag ccctaccatg ccacattcct
     5521 acgattacga tgccatagta ataggttccg gccccggcgg cgaaggcgct gcaatgggcc
     5581 tggttaagca aggtgcgcgc gtcgcagtta tcgagcgtta tcaaaatgtt ggcggcggtt
     5641 gcacccactg gggcaccatc ccgtcgaaag ctctccgtca cgccgtcagc cgcattatag
     5701 aattcaatca aaacccacta tacagcgacc attcccgact gctccgctct tcttttgccg
     5761 atatccttaa ccatgccgat aacgtgatta atcaacaaac gcgcatgcgt cagggatttt
     5821 acgaacgtaa tcactgtgaa atattgcagg ggaacgctcg ctttgttgac gagcatacgt
     5881 tggcgctgga ttgcccggac ggcagcgttg aaacactaac cgctgaaaaa tttattattg
     5941 cctgcggctc tcgtccatat catccaacag atgttgattt cacccatcca cgcatttacg
     6001 acagcgactc aattcttagc atgcaccacg aaccgcgcca tgtacttatc tatggtgctg
     6061 gagtgatcgg ctgtgaatat gcgtcgatct tccgcggtat ggatgtaaaa gtggatctga
     6121 tcaacacccg cgatcggctg ctggcatttc tcgatcaaga gatgtcagat tctctctcct
     6181 atcacttctg gaacagtggc gtagtgattc gccacaacga agagtacgag aagatcgaag
     6241 gctgtgacga cggtgtgatc atgcatctga agtcgggtaa aaaactgaaa gctgactgcc
     6301 tgctctatgc caacggtcgc accggtaata ctgattcgct ggcgttacag aacattgggc
     6361 tggaaactga cagtcgcgga cagctgaagg tcaacagcat gtatcagacc gcacagccgc
     6421 acgtttacgc ggtgggcgac gtgattggtt atccgagcct ggcgtcggcg gcctatgacc
     6481 aggggcgcat tgccgcgcag gcgctggtga aaggtgaagc caccgcacat ctgattgaag
     6541 atatccctac cggcatttac accatcccgg aaatcagctc tgtgggcaaa accgaacagc
     6601 agctgaccgc gatgaaagtg ccatatgaag tgggccgcgc ccagttcaaa catctggcac
     6661 gtgcacaaat cgtcggcatg aacgtgggca cgctgaaaat tttgttccat cgggaaacaa
     6721 aagagattct gggcattcac tgctttggcg agcgcgctgc cgaaattatt catatcggtc
     6781 aggcgattat ggaacagaaa ggtggcggca acactattga gtacttcgtc aacaccacct
     6841 ttaactaccc gacgatggcg gaagcctatc gggtagctgc gctaaatggc ttaaaccgcc
     6901 tgttttaaca ctttatcgaa atggccatcc attcttgcgc ggatggcctc tgccagctgc
     6961 tcatagcggc tgcgcagcgg tgagccagga cgataaacca ggccaatcgt gcggcgaggt
     7021 tccggcttaa tacacggcaa ataaacaacc ccatcgcgtt tgcgctccgg cggcacagcc
     7081 agcgcaggca gtaaagtgat cccgctacct gccgccacca tgttacgcag tgtctccagg
     7141 ctggtcgcgc ggaagtgtgt atcttcatcc gccccggctt caaagcagaa acccattgcc
     7201 tgatcgcgca aacagtgacc atcttccagc atcagcagtt tttcccctgc cagatcggcc
     7261 atcggtacgc attcgcggtt cgcccacggg tgatcttcat agatagccag caacattggc
     7321 tcatcaaaca acggcacttc aatgaatgct tcgctctctt tcaccagcgc gaggatcacg
     7381 caatcgagtt tgccactgtc cagttgcgcc agtaactggt gggtctgtgc ttcatgcaga
     7441 tacatttcca gctttggaaa ggtctggtgc agcatcggga taatatgcgg tagcaggtac
     7501 ggtccaaccg tgggaatcaa accaatatgc agcggtccgg acatcgtctc gccctgctgg
     7561 cttgccatct ctttaaggac tttcacctca cgcagcacgg tacgcgcctg atccaccagc
     7621 agcattcccg cctgggtgaa caacacttta cggctggtcc gctccagcaa catcacgccc
     7681 aactcatctt ccagcttacg aatttgcccg ctaagcgtcg gctggctaac gtggcaggaa
     7741 tctgccgcac gccgaaaatg gcggtgttca gccaatgcca ccaggtactc aagatcacga
     7801 atattcatta tccatcctcc atcgccacga tagttcatgg cgataggtag aatagcaatg
     7861 aacgattatc cctatcaagc attctgactg ataattgctc acagaaacgg agcggcacct
     7921 cttttaaccc ttgaagtcac tgcccgtttc gagagtttct caactcgaat aactaaagcc
     7981 aacgtgaact tttgcggatc tccaggatcc gctttttttt gccataaaaa agcccggcga
     8041 taagccaggc tcaaatttgt acatataaat gttcttaccc caaccgagcc tgcgcaaaag
     8101 caatcgcctg cgccacctgc tgcggtgaga cgccgccttt tgccgcacgc ttgtcgaggc
     8161 acgattgcag cgacagaatc ggatagacat cttcgtcaat cacctgactg aatttctgca
     8221 actcactgag cggcagatct tccagcggtt tgccctgacg aatggcttcc accaccgctt
     8281 caccaacaat atggtgcgcc tcgcggaacg gtacgccttt cgccaccaga taatccgcca
     8341 gttcggtggc gttggcgtaa ccctgctgag ccgcttcctg gcaacgtgga cgtttcacct
     8401 gaatgccgtc cagcaccagc gccgccatat gcaggcagtc cagccaggta tcgagcgcgt
     8461 cgaacagacc ttctttgtct tcctgcatat ctttgttgta agccagcggc aaacctttca
     8521 gcgtcatcat catgccggtt aacgccccct gcacccgacc gcatttaccg cgaatcagct
     8581 ccagcgcatc cgggtttttc ttctgcggca ttaatgatga accggaagtc acgcggtcag
     8641 aaagctccac aaaccccgct tcgccggtgt taaagaaaat cagatcttca gcaaaacgcg
     8701 acagatgcac catgccgata gcggcagcag aaagcagttc caacacatgg tcacggtcag
     8761 aaacgctgtc gagactgtta cgggtcgccg aagcaaagcc cagccagcct gctaactgtt
     8821 cacggtcgat ttcataggcc gttcccgcca gcgcgccaca gcctagcggg ctgacatcca
     8881 gacgcttaag cgcatcctgc aaacggcttt catcacgcgc cagcatctca acataggcca
     8941 ggcaccagtg cgcgaacgtc accggctggg cgcgttgcag gtgagtgtaa cctggcatta
     9001 ccgcgtcctg attgttttgt gcggtttcca ccagcgccga ttgcagctgc cggttagccg
     9061 tcagtaactc gctaacggta tctttgcacc acagtttcag gtcagtcgct acctgatcat
     9121 tacggctacg cccggtatgc agctttttgc ctaactggcc cactttgtcg atcagtttgc
     9181 cttccaccca gctatggata tcttcggcgt cgctttcaag gatttgttgt ggcctggcgc
     9241 gaacatcttc cagcaacacg ttcagcgcct cttccagttg cgcctgctct tctgcggtta
     9301 acacgcctac cgtgaccagg gctttggacc aggccacaga gccaacaata tcctgctccg
     9361 ccagacggta atcaaagcgc agtgagtcgt tgaattgttt gaaccgttga tctgctgcct
     9421 gggtaaaacg cccgccccaa agtgccataa ctctgtttcc ttattttgaa attcaatgcc
     9481 ggaaagctgc gcctccggcc aacaaaactt aagctaaaat ccgcgtaccc atcggcatac
     9541 cgttaaacag tgccggaagc tgctccgcat gacgccagga ggcgatatct accggacggc
     9601 ccagcgtgcg ggccgcatcc agcgccgcgt tcactttcac tatcatgccg tcagtaataa
     9661 tgccctgctc aatcagttgt tctgctttcg cggcggtcat ttcggcaatg cgttgccctt
     9721 tgccgtcgag aatgccgctg acgtcggaga gcaaaatcag atccgcgccc agcgttgccg
     9781 ccagcgccgt tgccgcctgg tcggcattga cgttcatcag ttgcccttcg tctgttacgc
     9841 caatggagct gaccaccggc agataaccgt tctccagcaa ggagttgata agcttaggcg
     9901 aacctggctg cgccagtcca acatgaccta actcttcatc aagctgggtc actttgacgc
     9961 tgtcgccgtc accgagaaac aaacctacgg ccgcaatctg atgtttcttc gcccatgcca
    10021 acagggtttt atttgccgtt cccgccagtg ctccggtgat aatgtctatc tgatcagcag
    10081 gcgtcacccg caggccgttt ttctttttca ccggcagatt tagccctttc atcagctcat
    10141 ccaccacgca accgccgccg tgcacaatca ccagcggacg ctgatgtgac tcacgataat
    10201 tcaccagtgc gctaaacaga cgttccagcg cctcttcact atccagcagt acgccgccca
    10261 gtttgataat taatggattc atcattgcac ccttaaataa gagactgcgt ttccgcatag
    10321 ccgaaacgaa tattggcgca ctgtaccgct tgtgccgccg cgcctttcag taagttgtct
    10381 tcggtcgcca caataatcaa atgctcgccc tgaacggcaa acccgatatc gcaaaatggc
    10441 agcccaacga catttttcag cgccggaacg cctttgtcat acagccgcac cagcggttta
    10501 tgggcatacg cctgctgtaa cacttgcgcg acttgcgcct gggtcacacc cgatttcagg
    10561 cggcaggtaa tggtttcgag aatgccgcgc gggaaattgc ccagatgtgg ggtgaagata
    10621 acgtcagcac cgaggtgtgt ggcgatctct ggttgatggc gatgagtaaa gacgccatac
    10681 ggttgcaggc taacttcaca aaagctgttt gaaatggccg ctttacgccc tgcaccgctc
    10741 acgccgctgg tggcgttgat caccggccac tgattgaggt caagaagatc ggcatcaatc
    10801 aacggtttca gcgccagctg tgccgccgtc ggataacagc ccggcaccgc aatcaaattc
    10861 gcttctttta atttattacc gcaccactcc gccagaccgt aggctgcctg ttccaacagt
    10921 tccgggtatt gatgggtaaa gccgtaatat ttttcataga aggtggcgtc gttaacacga
    10981 aacgcgccgg aaaggtcgaa caccacgcag cccgcttcaa gaaactgcgg cgctaaatcg
    11041 tggctaactt catgggcggt ggcgagaaac actacgtcca cccctgggct aaactcgctg
    11101 atatccgaca tcggctgcaa cggcagatca acgatgcctt ttagctgcgg atgcaaatcg
    11161 gagattaact ttcccgcatc attgctttgc gctgaaacag tcaaagcggt tatgttcata
    11221 tgcggatggc gatttacata ggtcactagc tctgcgccag cgtagccgct ggcacccaca
    11281 atcagcgtat tcaacatcgg ggctattcac cttcttatgt ctggttgcca ggttaaacgt
    11341 aaaacattca ccttacggct ggtgggtttt attacgctca acgttagtgt atttttattc
    11401 ataaatactg catgaatatt gatactatca tgaccagagg tgtgtcaaca atgaaaaaca
    11461 aattaccgcc atttatcgag atttaccgcg ctctgattgc cacaccttca ataagcgcca
    11521 cggaagaggc actcgatcaa agcaatgcag atttaatcac tctgctggcg gactggttta
    11581 aagatttggg cttcaatgtg gaagtgcagc ctgttccagg aactcgcaac aaattcaata
    11641 tgctggcaag tatcggacag ggggctggcg gcttgttgct ggcggggcat accgatacgg
    11701 tgccatttga tgacggtcgc tggacgcgcg atccgtttac actgacggag catgacggca
    11761 agctttacgg cttaggcacc gccgacatga aaggcttttt tgcgtttatc cttgatgcgc
    11821 tacgcgatgt cgacgtcacg aaactgaaaa aaccgctcta cattctggcg actgctgatg
    11881 aagaaaccag tatggccgga gcgcgttatt ttgccgaaac taccgccctg cgcccggatt
    11941 gcgccatcat tggcgaaccg acgtcactac aaccggtacg cgcacataaa ggtcatatct
    12001 ctaacgccat ccgtattcag ggccagtcgg ggcactccag cgatccagca cgcggagtta
    12061 acgctatcga actaatgcac gacgccatcg ggcatatttt gcaattgcgc gataacctga
    12121 aagaacgtta tcactacgaa gcgtttaccg tgccataccc tacgctcaac ctcgggcata
    12181 ttcacggtgg cgacgcttct aaccgtattt gcgcttgctg tgagttgcat atggatattc
    12241 gtccgctgcc tggcatgaca ctcaatgaac ttaatggttt gctcaacgat gcattggctc
    12301 cggtgagcga acgctggccg ggtcgtctga cggtcgacga gctgcatccg ccgatccctg
    12361 gctatgaatg cccaccgaat catcaactgg ttgaagtggt tgagaaattg ctcggagcaa
    12421 aaaccgaagt ggtgaattac tgtaccgaag cgccgtttat tcaaacgtta tgccctacgc
    12481 tggtgctcgg tccgggttcc atcaatcaag ctcatcagcc tgatgaatat ctggagacgc
    12541 ggtttattaa acccacccgt gagttgatta tccaggtaat tcaccatttt tgctggcatt
    12601 aaaacgtagg ccggataagg cgctcgcgcc gcatccggca ccgttgccaa actcctgtgc
    12661 cgcaataatg tcggatgcga tgcttgcgca tcttatccga cctacacctt tggtgttatg
    12721 tatggcgatt ttttaacatt tccataagtt acgcttattt aaagcgtcgt gaatttaatg
    12781 gcgtaaattc ctgctattta ttcgtttgct gaagcgattt cgcagcattt gacgtcaccg
    12841 cttttacgtg gctttataaa agacgacgaa aagcaaagcc cgagcatatt cgcgccaatg
    12901 cgacgtgaag gatacagggc tatcaaacga taagatgggg tgtctggggt aatatgaacg
    12961 aacaatattc cgcattgcgt agtaatgtca gtatgctcgg caaagtgctg ggagaaacca
    13021 tcaaggatgc gttgggagaa cacattcttg aacgcgtaga aactatccgt aagttgtcga
    13081 aatcttcacg cgctggcaat gatgctaacc gccaggagtt gctcaccacc ttacaaaatt
    13141 tgtcgaacga cgagctgctg cccgttgcgc gtgcgtttag tcagttcctg aacctggcca
    13201 acaccgccga gcaataccac agcatttcgc cgaaaggcga agctgccagc aacccggaag
    13261 tgatcgcccg caccctgcgt aaactgaaaa accagccgga actgagcgaa gacaccatca
    13321 aaaaagcagt ggaatcgctg tcgctggaac tggtcctcac ggctcaccca accgaaatta
    13381 cccgtcgtac actgatccac aaaatggtgg aagtgaacgc ctgtttaaaa cagctcgata
    13441 acaaagatat cgctgactac gaacacaacc agctgatgcg tcgcctgcgc cagttgatcg
    13501 cccagtcatg gcataccgat gaaatccgta agctgcgtcc aagcccggta gatgaagcca
    13561 aatggggctt tgccgtagtg gaaaacagcc tgtggcaagg cgtaccaaat tacctgcgcg
    13621 aactgaacga acaactggaa gagaacctcg gctacaaact gcccgtcgaa tttgttccga
    13681 tccgttttac ttcgtggatg ggcggcgacc gcgacggcaa cccgaacgtc actgccgata
    13741 tcacccgcca cgtcctgcta ctcagccgct ggaaagccac cgatttgttc ctgaaagata
    13801 ttcaggtgct ggtttctgaa ctgtcgatgg ttgaagcgac ccctgaactg ctggcgctgg
    13861 ttggcgaaga aggtgccgca gaaccgtatc gctatctgat gaaaaacctg cgttctcgcc
    13921 tgatggcgac acaggcatgg ctggaagcgc gcctgaaagg cgaagaactg ccaaaaccag
    13981 aaggcctgct gacacaaaac gaagaactgt gggaaccgct ctacgcttgc taccagtcac
    14041 ttcaggcgtg tggcatgggt attatcgcca acggcgatct gctcgacacc ctgcgccgcg
    14101 tgaaatgttt cggcgtaccg ctggtccgta ttgatatccg tcaggagagc acgcgtcata
    14161 ccgaagcgct gggcgagctg acccgctacc tcggtatcgg cgactacgaa agctggtcag
    14221 aggccgacaa acaggcgttc ctgatccgcg aactgaactc caaacgtccg cttctgccgc
    14281 gcaactggca accaagcgcc gaaacgcgcg aagtgctcga tacctgccag gtgattgccg
    14341 aagcaccgca aggctccatt gccgcctacg tgatctcgat ggcgaaaacg ccgtccgacg
    14401 tactggctgt ccacctgctg ctgaaagaag cgggtatcgg gtttgcgatg ccggttgctc
    14461 cgctgtttga aaccctcgat gatctgaaca acgccaacga tgtcatgacc cagctgctca
    14521 atattgactg gtatcgtggc ctgattcagg gcaaacagat ggtgatgatt ggctattccg
    14581 actcagcaaa agatgcggga gtgatggcag cttcctgggc gcaatatcag gcacaggatg
    14641 cattaatcaa aacctgcgaa aaagcgggta ttgagctgac gttgttccac ggtcgcggcg
    14701 gttccattgg tcgcggcggc gcacctgctc atgcggcgct gctgtcacaa ccgccaggaa
    14761 gcctgaaagg tggcctgcgc gtgactgaac agggcgagat gatccgcttt aaatatggtc
    14821 tgccagaaat caccgtcagc agcctgtcgc tgtacaccgg ggcgattctg gaagccaacc
    14881 tgctgccacc acctgagccg aaagagagct ggcgtcgcat tatggatgaa ctgtcagtca
    14941 tctcctgcga tctctaccgt ggctacgtac gtgaaaacaa agattttgtg ccttacttcc
    15001 gctccgctac gccggaacaa gaattgggca aactgccgtt gggttcacgt ccggcgaaac
    15061 gtcgcccaac cggcggcgtc gagtcactac gcgccattcc gtggatcttc gcctggacgc
    15121 aaaaccgtct gatgctcccc gcctggctgg gtgcaggtac ggcgctgcaa aaagtggtcg
    15181 aagacggcaa acagagcgag ctggaggcta tgtgccgcga ttggccattc ttctcgacgc
    15241 gtctcggcat gctggagatg gtcttcgcca aagcagacct gtggctggcg gaatactatg
    15301 accaacgcct ggtagacaaa gcactgtggc cgttaggtaa agagttacgc aacctgcaag
    15361 aagaagacat caaagtggtg ctggcgattg ccaacgattc ccatctgatg gccgatctgc
    15421 cgtggattgc agagtctatt cagctacgga atatttacac cgacccgctg aacgtattgc
    15481 aggccgagtt gctgcaccgc tcccgccagg cagaaaaaga aggccaggaa ccggatcctc
    15541 gcgtcgaaca agcgttaatg gtcactattg ccgggattgc ggcaggtatg cgtaataccg
    15601 gctaatcttc ctcttctgca aaccctcgtg cttttgcgcg agggttttct gaaatacttc
    15661 tgttctaaca ccctcgtttt caatatattt ctgtctgcat tttattcaaa ttctgaatat
    15721 accttcagat atccttaagg aattgtcgtt acattcggcg atattttttc aagacaggtt
    15781 cttactatgc attccacaga agtccaggct aaacctcttt ttagctggaa agccctgggt
    15841 tgggcactgc tctacttttg gtttttctct actctgctac aggccattat ttacatcagt
    15901 ggttatagtg gcactaacgg cattcgcgac tcgctgttat tcagttcgct gtggttgatc
    15961 ccggtattcc tctttccgaa gcggattaaa attattgccg cagtaatcgg cgtggtgcta
    16021 tgggcggcct ctctggcggc gctgtgctac tacgtcatct acggtcagga gttctcgcag
    16081 agcgttctgt ttgtgatgtt cgaaaccaac accaacgaag ccagcgagta tttaagccag
    16141 tatttcagcc tgaaaattgt gcttatcgcg ctggcctata cggcggtggc agttctgctg
    16201 tggacacgcc tgcgcccggt ctatattcca aagccgtggc gttatgttgt ctcttttgcc
    16261 ctgctttatg gcttgattct gcatccgatc gccatgaata cgtttatcaa aaacaagccg
    16321 tttgagaaaa cgttggataa cctggcctcg cgtatggagc ctgccgcacc gtggcaattc
    16381 ctgaccggct attatcagta tcgtcagcaa ctaaactcgc taacaaagct actgaatgaa
    16441 aataatgcct tgccgccgct ggctaatttc aaagatgaat cgggtaacga accgcgcacc
    16501 ttagtgctgg tgattggcga gtcaacccag cgtggacgca tgagtctgta cggttatccg
    16561 cgtgaaacca cgccggagct ggatgcgctg cataaaaccg atccgaatct gaccgtgttt
    16621 aataacgtgg ttacgtctcg tccgtacacc attgaaatcc tgcaacaggc gctgaccttt
    16681 gccaatgaaa agaacccgga cctgtatctg acgcagccgt cgctgatgaa catgatgaaa
    16741 caggcgggtt ataaaacctt ctggatcacc aaccagcaga cgatgaccgc ccgcaatacc
    16801 atgctgacgg tattttcgcg ccagaccgac aagcagtact acatgaacca gcaacgtacg
    16861 cagagtgcgc gtgaatacga caccaacgtg ctgaagccgt tccaggatgt gctgaatgac
    16921 cctgcgccga agaaactgat catcgttcat ctgctgggta cgcatatcaa atacaaatac
    16981 cgctacccgg aaaatcaggg caagtttgat ggcaataccg atcatgttcc gccaggatta
    17041 agcgcagaag agctggaatc atataacgat tatgacaacg ctaacctgta taacgatcat
    17101 gtggttgcca gcctgattaa agactttaaa gcggcagacc cgaacggatt cctggtttac
    17161 ttctctgacc acggtgaaga ggtttacgac acgccgcctc ataaaaccca ggggcgtaat
    17221 gaggacaacc cgacgcgtca tatgtacacc attccgttcc tgctgtggac gtcggaaaaa
    17281 tggcaagcga ctcatccccg tgatttctca caggatgtcg atcgtaaata cagcctggcg
    17341 gaactgatcc acacctggtc agatttggcg ggcttatctt acgacggtta cgatccaacc
    17401 cgttcagtgg tgaatccgca gttcaaagaa actacccgct ggattggtaa cccgtacaag
    17461 aaaaacgcgc tgatcgatta cgacacactg ccgtatggcg atcaggtggg taatcagtaa
    17521 ttatcagcca ggcgctattt gccggatgcg acgcttgacg cgtcttatcc ggcctacaga
    17581 tcgcggcggt aggctggata aggcgtttac gccacatccg gcaatacaac tgccgcaatg
    17641 tttacatcga actcccctca ggccacactt ttcaggtggc cttttttaat aatgccagtt
    17701 actgttttcc cgtctacata cctaagggaa taacatgtat cacgacgtca gctatctgct
    17761 ctcccgcctg atcaacggcc cgctgtccct gcgccagatt tactttgcca gcagtaacgg
    17821 cccggtgccc gatcttgcgt atcaggtcga ttttccacgg ctggaaattg tgctggaagg
    17881 tgagtttgtt gataccggcg ctggagcaac gttagttccc ggcgatgtgc tgtacgttgc
    17941 cgctggtggc tggaatttcc cacaatggaa aacacccgct accaccttta gcgtgctgtt
    18001 tggcaaacag caactcggct tcagcgtcgt gcaatgggat ggcaaacaat atcaaaatct
    18061 ggcgaagcaa cacgtcgccc ggcgcggccc acgcataggt tcttttcggc tacaaacgct
    18121 caatgaaatg caaatgcagc cgcaggagca gcaaacggca aggcttatcg tcgccagcct
    18181 gcttagccac tgccgcgatt tgcttggcag ccaaatccag accgcctcac gcagccaggc
    18241 actatttgaa gctattcgcg attatatcga cgaacgctat gcctccgcgc ttacccgcga
    18301 atctgttgcg caggcgtttt atatttcgcc aaattacctc tcgcacctgt ttcaaaaaac
    18361 gggggccatt ggttttaacg aatacctgaa tcacacgcga ctggagcacg ctaagacgtt
    18421 attgaaaggc tatgacctga aggtaaaaga agtggcgcac gcctgcggct ttgtcgacag
    18481 caactacttc tgccgtctgt ttcgtaaaaa caccgaacgc tcgccgtcgg aatatcgtcg
    18541 gcagtaccac agccaactga cagaaaaacc gactactcca gaataagatg ggtttgctgc
    18601 ggcgcagaaa gcactttgcg caccgcgctc attacccgct gcggctcacg caggaatgcg
    18661 tagatgctgc attgcacata gcgacaatgt tcaaatcgct cggcaccggc aagctcgata
    18721 tccgtaatca acagagcaac atccgcccga cggatatcct cgtccgctaa acgattctcc
    18781 gttcccagcg cgccctgagt ttcaatgctg actccccact tctctaacag gcacaacttt
    18841 tccagccgtt ccgccgccat ataagtatgc gccacgccac tgacgcaggc ggttactgcc
    18901 accaggtatg ccattttaac ctcccacggt aacctgaaat ccggcccgtt ctgccatttc
    18961 gcgcatcgtt gtcacatcgg ctgacgacgg cgcaggcacc tctttcatcg accatgtttt
    19021 ccccagcagg cggtatttcg gttcgccgta ctgatgaaac ggtaacagat ggatctgcct
    19081 gattttcagc gggatcagta catccagcgc ctgctgcata ttctcccggc tgagcgtgaa
    19141 accagggatc aacggtaaac gcgggatcac gttgacaccc tcactcacca gcaaacgcag
    19201 attctccagc acgcgtggca ggttcatctt caccacatcc cgcgcctgag tcgcgtccat
    19261 aatttttaaa tcgaacagca cttcatcgca caatttcgcc agcggtaaca gcttgcttgc
    19321 tggcgcgtct ccggcagttt caatggcgca tgacacaccc cacagccgca gtcgctgtaa
    19381 aaaacgggta gcaaactccg cctgcattaa cacttcgccg ccagaaagcg tgacgccgcc
    19441 gccggacgtg cgaaaaaaaa tgtcatcttt catcacttcc cgttccagag cgtcaaggct
    19501 gatatcgcga ccaatccgtt caaacgcccc ggaggggcat tcatccgcat cacgcaaaca
    19561 tttcgcacag tgcagacatt tcgcctctct gcgtaccgtc tggattttgc cggagatcga
    19621 ctccggatta gcacaccacg ggcaaagatg cggacagcct ttaaaaaaga ccaccgtacg
    19681 aatgccctca ccgtcattca gtgaataacg ctgaatgttg aaaatgcgcg ccacatcatt
    19741 gcggcgcgtt tccacaacgt tacagctgat gcgctgtccg gcggatgatg tcatcctgga
    19801 tctccttcga cagttcgaca aagaaggcgc tgtatccggc aacgcgcacc accagcccgg
    19861 cataatcttg tgggcgctgt tgcgcttccc gcaacgtgtc ggcgttcacc acgttaaact
    19921 gaatatgttg taacttaagc tgggtaaacg cccgtaagaa gtcggccagt ttgcgtaatc
    19981 ccgcttcacc ttccagggtc gccggagtga atttcacgtt cagcaatgta ccgttagaca
    20041 gcagtgtgtt atcgagctta ctgactgact tcagtaccgc cgttggccct tgtgcgtcct
    20101 gacccagcat aggtgacaag ccgccgtctg ccagctgttc tccggcaaaa cgaccgtctg
    20161 gcgtcgcgcc aaccaccgat cccaacggaa cgtgagcaga aacggtatac gatcccggcg
    20221 tgaagtagcc gccgcgcggg ttctggtatt tttccacttc tttgcagtag tggcgcaaca
    20281 gttcggcgct aatgttatcc acctcgtcga tatcgttacc gtatttctca aagcggttaa
    20341 ttaagcgagc gcggactttt tcgccttctg gcgttgcaaa gttggctttt aataccgaca
    20401 gcaattcgtc aaaacttaaa cgctgttgat caaaaaccat ccctttcaac gcatggagag
    20461 aatcgctcag gttggcgata ccgatcccct gtacgccgga gaagttataa cgcgcgccgc
    20521 catcggtaat atcgcggcct ttttccagac aatcgctgat aaaagacgag agcagcggta
    20581 caggtgccca gtcgcgatgg ccgatatcgc aaatattact gccttcaacc atcagggtga
    20641 tgtagtggct gatcttggca cggatctgtt ccagtaaacc ttcataagtc agcgcggcat
    20701 tgccttcatt ttcatgcagg cagatttcca tcactttcag caggttaaac atcgcgatgt
    20761 catgcaagcc gtaggttctg ccgggaatag ataattccac acagcccact acggaatagt
    20821 cgcgcgcatc ttccagcgaa acgccacggt tgaggaacgc tggcaccacc acttcatcgt
    20881 taaagatttg cggaataccg gtgccgaggc gaatggtttc ggcggttttc atcaggaacg
    20941 gcgtgtcgat caaggcgtta gtgcgcacgc cgaggttcgg ttgcggtaat tgcacgcttt
    21001 gataggcgtc aaggcaaagg aacgaaagca cgttcaccgc gctacgtccg ttctcggtta
    21061 acccgccgag cagtgcggta tagccggtcg ggaaaccggc gaaataacgc gcgctgctgg
    21121 tggagcgcaa cagcacgatg tcgttgcatt tcacccataa agattcgagc agttctttca
    21181 ggaacgccgc atcttcgccc tgggttaatg atgtctgata gaacggcaac atatactggt
    21241 cgaagcgccc caacgatagc gaactggcgt tggattcgta ttgcagaatg atgttcatgt
    21301 accagaataa ctggcacgcc tgccagaacg tctgcggctt atgttgcgcg ttatggcggg
    21361 agatctccgc aatagtcagc agctcttcgc gacgctgggc atctgtgcag tttgccgcca
    21421 tcgtttccgc cagttcggcg taacgcaaaa tgtgtttctg cgaggcttcc agcagtaaca
    21481 gcgctgcctg ataaaagtga ttctccggct gttgctgaca atgttgctgc atctgtgcta
    21541 ccagctcccc cagcccgtga ttcagcaggc gtgggtaatc aataataatg tgcccctggc
    21601 ctttatctgt ctggttgatg ctgaaaatct gcgtgctggt cgcggctttt acttcatctg
    21661 tcatctgccc gttgatgaaa tctttcatcg aacgtttttc ccagtacggg aacaactctt
    21721 cgcggtagat acgtttatct tcttcgctga tagcaaagcg gtcctgcgga cgcgtcggga
    21781 attgatccag ctctttcagc agccagtaag ggtccatttc cggcgacata atcccggcgc
    21841 gcggttttac ggtgcggtta ccggcaatca gttcttcatc acgaatcgaa atttcaacat
    21901 gttcaaggat atacgctgtt gctttcgccc ggcgcaatat caccggttcg ccttcggttt
    21961 gccgatggct ggcggtataa agcagcgccc gctccagcga gatttcacgg gtattggaaa
    22021 acagtgcagt tttgaggcga gagatacgat tcgtcatgag gaactcctgt gggaaagcga
    22081 acccgccctt gcgagcggga gaagaactta agcggtttgc gccaggtgag cttcaatttt
    22141 gctcataatg gcgtcagcac gttttaccgc atcgctgatg ttgacgcgca cgatggtctt
    22201 gccagcaaaa cgctcttcaa acttgatgcc gatatctttg gtcagaatca ccatatcagc
    22261 gctcgccacg tcttccgctg tcagttcatt ttccagacca attgacccct gggtttctac
    22321 tttcacttcc cagcctttcg ctttcgcggc actttccagc gcctctgctg ccatataggt
    22381 atgggcaaca cctgaaggac atgcggttac tgcaataatt ttcgtcatgg cttgttcctc
    22441 aattaattaa tttcaaaatc caaatccagg tcgtcttctt tttcatcagt cgaagaccca
    22501 tttttacgcg ccagactttt cagcacgttc acacaaacag ccgtcaccac tgctccgaca
    22561 gccactgcgg cgatataacc cagcttgcct tcaaccaccg gcagtacaat cagaccaccc
    22621 cagcctgcat agcactgcgc accgaccagc gccgcagtta ccgcaccaca aacagaacca
    22681 accatgatgg aaggaatgac acgcagcgga tcggcagcgg cgaaaggaat cgccccttca
    22741 gtaaccccaa cgcaccccat caccagcgcc gctttaccag tttcgcgctc ttctgcggag
    22801 aaatttttac gaccaatcag cgtcgccagc cccatcccca gcggtggaac acaaatccca
    22861 acagcggcaa tagccaccac ggtataaaca ccctgagcaa cgcaaatcag catgaaggca
    22921 taggccactt tgttaaccgg accgcccata tcgaacgcca gcatcagacc catgatcacc
    22981 gccagcataa caatgctgcc ctgctgcatc ccctgaagcc actgagtcag gctgttggtc
    23041 aacgccccta caggctcgcc caagccccac atcatgatgc ctgcggtaat cagtgtgccg
    23101 acgataggaa tgatgaagat aggcatcacc gagcgcagaa ctttatgcac cggaattttc
    23161 ttcaggtaat gcaccacgat gccgccgata atcccggcga tcagcgcacc aaagaagccc
    23221 gcaccaaagc tgttaccaac ccaggcaccg atagcgcacg gagccagcgc agaacgctct
    23281 gcaatggagt aaccaatgta agcggcgagg aaaggcacca tcagcgtcaa acccgcaacg
    23341 ccgatatcaa acagtttttt cagattcgga tcggctacgg catccggcac tgcgcctttg
    23401 ccatacaaca tgacggaaac cgccagcaaa ataccgcccg ataccacgaa gggaatcatg
    23461 tgtgaaacgc ccgtcattaa atgctgacgg gtatttttca ggatctgcac caactcattc
    23521 ataagctgct cccgggcttg caaaagccca tgtccatatt tttgatggag gaaacaatag
    23581 gcaatcgcag gcggagcaaa cagtggataa aaaggccatt tactggactt ttgtaatatc
    23641 agtacaaaaa tgcgatccgc ctcataactt gcgataatcc ggaagcatgc cgcaaaacgg
    23701 cgtcttgtga gacgcgtcgc aaattgacgg ttacattaca tttgtccagc ttttggcaga
    23761 tttgtcacat ggcaggccgt tgccgtgcgc tcttatcctg taaacagaaa accgtaccgg
    23821 gagagaggca atggccctga ttgtggaatt tatttgtgag ctacctaacg gcgtacatgc
    23881 acgtccggca agccacgttg aaacgctgtg taatactttt tcatcacaaa ttgagtggca
    23941 taacctgcgc actgaccgta aaggcaacgc caaaagcgcc ctggcgctga ttggcaccga
    24001 cacgctggtg ggcgataatt gccagttact gatttccggg gccgacgaac aggaagcgca
    24061 ccagcgttta agccaatggc tgcgcgatga attcccccac tgcgatgcgc cgctggcgga
    24121 agttaaatct gacgaactgg aaccactgcc ggtttcattg accaatctga atccgcaaat
    24181 tatccgcgcc cgcaccgtgt acagcggtag cgcaggcggc attctgacgc cgatctcttc
    24241 tttagatctc aatgcgctga gtaatcttcc cgctgccaaa ggcgttgacg ccgaacaatc
    24301 cgcactggaa aacggcctga cactggtatt gaaaaacatt gagtttcgtc tgctggatag
    24361 cgacggtgct accagcgcga ttctggaagc gcaccgatcc ctggctggcg atacttccct
    24421 gcgcgaacat ttactggcag gcgtcagcgc cggattaagc tgcgccgaag cgattgtcgc
    24481 cagtgcgaat cacttttgcg aagagttcgc ccgttccagc agcagctacc tgcaagaacg
    24541 tgccctggac gtacgcgacg tctgcttcca gttactccag caaatctacg gtgagcaacg
    24601 cttcccggca ccgggcaaac tgacgcagcc cgccatttgt atggctgatg aactgactcc
    24661 cagccagttc ctcgaactgg ataaaaatca cctcaaaggt ctgttactga aaagtggcgg
    24721 caccacctca cataccgtaa ttcttgcccg ttcgttcaac attccgacac tggttggtgt
    24781 ggatattgat gccctcactc cgtggcagca tcaaacgatt tatatcgacg gcaacgctgg
    24841 ggcgattgtg gttgagccag gggaagccgt agctcgttat tatcagcaag aagcccgcgt
    24901 acaggacgcc cttcgtgagc aacagcgtgt ctggctgacc caacaagccc gtaccgctga
    24961 cggtatccgt attgaaattg ccgctaacat cgctcactcc gtggaagcgc aggccgcatt
    25021 cggcaatggt gcggaaggcg ttggtttgtt ccgcactgaa atgctctata tggatcgcac
    25081 cagtgcaccg ggtgaaagcg agctgtacaa cattttttgt caggcgctgg agtctgccaa
    25141 cggacgcagc attattgtgc gcactatgga cattggcggt gataaacccg ttgattatct
    25201 gaacattccg gcagaagcta acccgttcct cggttatcgc gcggtgcgta tttatgaaga
    25261 atacgcgtcg ttgtttacca cacaactacg gtcaatcctg cgcgcctctg ctcacggcag
    25321 cctgaaaatc atgatcccga tgatctcctc aatggaagag atcttatggg tgaaagaaaa
    25381 actggcggaa gccaaacagc aactgcgtaa cgaacacatt ccgtttgatg agaagatcca
    25441 gctcggcatt atgctggaag tgccgtcggt gatgttcatc atcgatcaat gctgcgaaga
    25501 gattgatttc tttagtattg gtagtaatga cctgacgcaa tatctgctgg cggtggatcg
    25561 cgataacgct aaggttactc gtcactacaa cagcctgaat ccggcattct tgcgggcgct
    25621 cgattacgcc gtgcaggcgg tgcatcgcca gggcaaatgg attggtctgt gcggtgagct
    25681 gggagcgaaa ggttccgtgc tgccgttgct ggtcggttta gggctggatg aactcagcat
    25741 gagcgcaccg tcaattccgg cggcgaaagc gcgaatggcg caacttgata gccgtgagtg
    25801 ccgccagttg ctcaaccagg caatggcctg ccgtacttcg ctggaagtgg aacatctgct
    25861 ggcgcaattc cgcatgaccc aacaggacgc accgctggtc accgccgagt gcatcacgct
    25921 ggaaagtgac tggcgcagca aagaagaagt gctcaaaggc atgaccgata acctgctgct
    25981 ggcgggccgc tgccgctatc cgcgtaaact ggaagccgac ttatgggcgc gcgaggccgt
    26041 tttctctacc ggtctgggct ttagttttgc cattccccac agcaaatcag aacacattga
    26101 gcaatccacc atcagcgtag cgcgtctgca agcgccggtg cgctggggcg atgatgaagc
    26161 gcaattcatc attatgttaa ccctgaacaa acacgctgcg ggcgatcagc atatgcgcat
    26221 tttctcgcgc ctcgctcgcc gcatcatgca cgaagaattc cgtaacgcgc tggttaacgc
    26281 cgcctctgcc gacgctatcg ccagcctgct gcaacatgaa ctggaactgt aaaaggaaac
    26341 atcatggaac tgtatctgga caccgctaac gtcgcagaag tcgaacgtct ggcacgcata
    26401 ttccccattg ccggggtgac aactaacccg agcattatcg ctgccagcaa ggagtccatc
    26461 tgggaagtgc tgccgcgtct gcaaaaagcg attggtgatg agggcattct gtttgctcag
    26521 actatgagtc gtgacgcgca gggtatggtg gaagaagcga agcgcctgcg cgacgccatt
    26581 ccgggcgttg tggtgaaaat cccggtgact tccgaaggtc tggccgccat taagttgctg
    26641 aaaaaagagg gtattactac acttggcacc gctgtatata gcgccgcaca agggttatta
    26701 gccgcactgg caggggcaaa atacgttgct ccgtatgtta accgcgtaga tgcccagggc
    26761 ggggacggca ttcgtacggt tcaggagctg caaacgctgt tagaaatgca cgcgccagaa
    26821 agcatggtgc tggcggccag ctttaaaacg ccgcgtcagg cgctggactg tttactggca
    26881 ggatgtgaat ccatcaccct gcccttagat gtagcgcaac aaatgctcaa cacccctgcg
    26941 gtagagtcag ctatagagaa gttcgaacac gactggaatg ccgcatttgg cactactcat
    27001 ctctaaagga gcaattatgg accgcattat tcaatcaccg ggtaaataca tccagggcgc
    27061 tgatgtgatt aatcgtctgg gcgaatacct gaagccgctg gcagaacgct ggttagtggt
    27121 gggtgacaaa tttgttttag gttttgctca atccactgtc gagaaaagct ttaaagatgc
    27181 tggactggta gtagaaattg cgccgtttgg cggtgaatgt tcgcaaaatg agatcgaccg
    27241 tctgcgtggc atcgcggaga ctgcgcagtg tggcgcaatt ctcggtatcg gtggcggaaa
    27301 aaccctcgat actgccaaag cactggcaca tttcatgggt gttccggtag cgatcgcacc
    27361 gactatcgcc tctaccgatg caccgtgcag cgcattgtct gttatctaca ccgatgaggg
    27421 tgagtttgac cgctatctgc tgttgccaaa taacccgaat atggtcattg tcgacaccaa
    27481 aatcgtcgct ggcgcacctg cacgtctgtt agcggcgggt atcggcgatg cgctggcaac
    27541 ctggtttgaa gcgcgtgcct gctctcgtag cggcgcgacc accatggcgg gcggcaagtg
    27601 cacccaggct gcgctggcac tggctgaact gtgctacaac accctgctgg aagaaggcga
    27661 aaaagcgatg cttgctgccg aacagcatgt agtgactccg gcgctggagc gcgtgattga
    27721 agcgaacacc tatttgagcg gtgttggttt tgaaagtggt ggtctggctg cggcgcacgc
    27781 agtgcataac ggcctgaccg ctatcccgga cgcgcatcac tattatcacg gtgaaaaagt
    27841 ggcattcggt acgctgacgc agctggttct ggaaaacgcg ccggtggagg aaatcgaaac
    27901 cgtagctgcg cttagccatg cggtaggttt gccaataact ctcgctcaac tggatattaa
    27961 agaagatgtc ccggcgaaaa tgcgaattgt ggcagaagcg gcatgtgcag aaggtgaaac
    28021 catccacaac atgcctggcg gcgcgacgcc agatcaggtt tacgccgctc tgctggtagc
    28081 cgaccagtac ggtcagcgtt tcctgcaaga gtgggaataa cctactccaa actcccggct
    28141 tgtccgggag tttgaacgca aaattgcctg atgcgctacg cttatcaggc ctacgcaatc
    28201 tctgcaatat attgaatttg cgtgcttttg taggccggat aaggcgttca ctccgcatcc
    28261 ggcactctgt gccagtaatc tgaaactccc acgttgttca ggaatttttt atccgcttct
    28321 ggacattttc tctacagaac aatcgttagc ccggaaggtc gaatctgtga ctcttatttt
    28381 cacggacgaa ccagatgaaa gcttcactgg cgctgctcag cctgctaacg gcatttacca
    28441 gccactcctt aaaatctccc gccgttccac caaccgtggt acaaatccag gccaatacca
    28501 accttgccat cgcagacggt gccagacagc aaatcggcaa cacgctattt tacgatccgg
    28561 cgtatgtgcg gcttacctat cctggcggtg atgtcccgca agaacgcggt gtttgttctg
    28621 atgtggtgat ccgcgcattg cgcagccaga aggtcgattt gcagaaactg gttcacgaag
    28681 atatggcgaa gaattttgct gagtacccgc aaaaatggca gttaaagcgc ccggacagca
    28741 acatcgatca ccgtcgggtg cctaatctgg aaacctggtt tagccgccat gataaaacac
    28801 gccccaccag caagaacccc agtgactatc aagcgggcga tattgtctcc tggcgactgg
    28861 ataacgggct ggcacatatt ggggtggttt cagatggctt cgcccgcgac ggtacgccgc
    28921 tggtgataca taacattggc gcaggcgcgc aggaagaaga tgtgctgttt aactggcgga
    28981 tggtggggca ttatcgttat tttgttaaat aagagatcgc cccacatgaa gaaaaaattc
    29041 agatcctttt tacactgacg gcttcttttt tcttacggct caccagcgcc agtgccagca
    29101 caatcagcac aataccgcta ccttcaacgg cccccggatt ctcgccgagc agccaccagg
    29161 aaaagagtac gccgcaaacg ggaacggcca gtgtgcttaa gctggcaata ctggcaggca
    29221 agtttttcaa tacaaacaac cataagctcc acgccagtgc cgtcgccaga atcgcactgt
    29281 aggccagcgc ccagaacacg gtgggctgcc agtcaatttc acgttgcggc accagtaaag
    29341 cgaccacact catcaccagc gccgcataca gcatctgcca ggatgttaac gacaataaat
    29401 ccacgcgcgg atgacgggca tacagacgtt tagcaacaat cgcgctcgcc ccccaactga
    29461 cgccggagag gattgccagc atggcacttt tcatcgaaga gaaatcgagt tgccacggct
    29521 gcaacaccaa aaataagccg aaagcggcaa tcagaatcgc gaaatattgc ccacgtcgca
    29581 ggcgttcacc gagaaacaac gcggcgaaaa tcaccaccca gaacggcatg gtatagctca
    29641 ggatcgccac cttccccgca cctccgctga ccaacgccca ctgcgccaga ccaaccatcc
    29701 cgcaggtttg taacagggca atggctaagg tgtatttaaa cggtgtcggg cgcattccgc
    29761 gaccacgtaa taaaaggacg atgaataaaa cgagagcgcc gaaaatgcag cgtaaggcgg
    29821 taaagtcgaa ggcaccgatg taactggtga cttgcttcat gaaaatccag ctataactcc
    29881 agataagtgt gagcacaacc aggccactga ttgccagtgg gttgctcttt cctgcggcag
    29941 acatgagaga tccagtgtgt agttttcgtt cgcccactat acgctggtaa agaacgttca
    30001 gcgactgcca gcaagcagcc gctgaacggg gtcagattac agcaggtcga aacggtcgag
    30061 gttcatcact ttcacccatg ccgccacgaa gtctttaaca aacttctcgt gggcatcgct
    30121 actggcgtaa acttccgcca ccgcacgcag gacggagtta gaaccaaaca ccagatccgc
    30181 acggctggcc gtaaatttca cttcgccggt ttcacggtca cggccttcga acagctcttt
    30241 cgattcgtcg gtcgctttcc actcgtaacg catatccagc aagttcacga agaagtcatt
    30301 gctcaatacg ccaacgcggt cagtgaagac gccgtttttg ctgccatcga agttggcacc
    30361 cagtacacgc atgccgccca ccagcgcagt catttccggc gcggtcagcg tcagttgctg
    30421 tgctttgtcg atcagcagtg actcggtggt ggaaacgtcc agacgagcgc gatagttacg
    30481 gaaaccgtca gcaattggct ccagcagctc aaacatctca atgtcagtct gatcctgacg
    30541 cgcatcaacg cgacccggcg caaacggtac atgaatgctc aaacctgcgg cgcttgcggc
    30601 tttctcaaca ccaaccacac cagccagcac tatgatatcc gccagcgagg ctttaccaga
    30661 ctctttctgg attttctcca gaacaggcag agcacgaacg gctgcggcgt tcacatccca
    30721 gtcgcgctgc ggcattaatg ccagacgcgc accgttggca ccaccgcgtt tgtcgccacc
    30781 acggaaggta gaagcagatg cccaggccac cgataccagc tcactaacag acagaccaga
    30841 atccgcaatc gcgaatttca gatcgataat gtcctgctcg gtcgggttgt agatcggctg
    30901 cggcagcgga tcttgccaga tcagatcttc tttcggcact tccggcccga tgtagcgaga
    30961 tttcggcccc atatccctgt gcgtcagttt gaaccaggca cgggcaaagg cttcgttgaa
    31021 cgcctgcgga tcgttgagga aacgacgaga gatcttctcg aactcaggat caaaacgcag
    31081 cgtcaggtcg gtcaccagca ttgtcggttt acgtttcttc gacggatcaa acggatccgg
    31141 gataatttcc ggtgcgtcta ccgcttcgaa ctggattgcg ccagccgggc tgcgggtctg
    31201 tacccactca tacttgaaca ggttctcgaa gaaatagttg ctccactggg tcggcgtctg
    31261 ggtccagact acttccagac cagaggtaat ggcatctgcg ccaacgccgc tgccgtaagt
    31321 gctcgcccaa cctaaacctt gttcttcaat cggtgcagct tctggatcag gacctacatt
    31381 tgatgtcgga ccggcaccgt gggttttacc cagcgtatga ccacccgcaa tcagcgccac
    31441 ggtttcttcg tcgttcatgc ccatgttgcc gaaggtcgcg cggatagctg ctgccgcaga
    31501 aagcggttcg ccgctgtgat ccgggccttc cgggttaacg taaatcagac ccatctcggt
    31561 tgcacccagc ggtgctttcg ccagcgcttc cggatgacgg tgagtcagcc aggctttttc
    31621 atcaccccag ttaacatcca gatccggttc ccagacgtct tcacgaccgg caccaaaacc
    31681 gaaggtacgg aagccggagt tttctagcgc cacgttaccc gcgaggataa acaggtcggc
    31741 ccaggagatt ttctgaccat atttctgttt gattggccac aacaggcgac gcgctttatc
    31801 gaggcttacg ttatccggcc aggagttcag cggtgcaaaa cgttgctgac cacgacccgc
    31861 gccaccgcgt ccatcgattg aacggtaagt ccccgcgccg tgccaggcca tacgaataaa
    31921 cagaccggcg taactgcccc agtcggctgg ccaccacggt tgagattctg tcaacagggc
    31981 tttcagatct tttttcaggc cgtagtaatc taatttgctg aattctttgc ggtagtcaaa
    32041 gtcctcaccc agtgggttag aacgattaga atgttggttt aacaggtcaa cacgaagttg
    32101 atttggccac cagtcgcgag tggttgtgcc cgcccccgca ctctggtcgt gaccgccctg
    32161 atggaacggg catttgccag tggctgtggt gttatggata tcgtctgacg tgctcatcaa
    32221 tgtgctcccc tctacagtgt taccgttacg atacacagcg ttagagagaa gttataattg
    32281 aattaatcca cggatgcgat agttgagatc ttacatattg ttggttaaag agatgtagat
    32341 caaattgatc ttaattaagc ttttttgatt atgtccgaat tcggacaaat gctttataaa
    32401 aaagggtttt agcttcatat ccttcaagta atgagggaga aaacaaggct gataacctta
    32461 ttttcccgcc ctcatttcga ggcagcattt tgtgctctgt ttaaaatttg tgatcactgt
    32521 gtgattttca caaaagccac actatttata aaccaggtcg aacccccagc gtatggcaaa
    32581 tcgcgtaact catttcagca cggttaagcg tatagaagtg gaaatctttc actccttcac
    32641 ggcttaaaat cttcaccata tccatggcaa tattcgcgcc aaccagtttg cgggtttcgg
    32701 catcatcatc cagaccgtcg aacatttgcg ccatccacgc cggaatacgc acgttggtca
    32761 tatcggcaaa tttcttcgcc tgtttaaagt tagataccgg caaaattccc ggaataattt
    32821 ccacatcaat gcccgccgat acacagcggt cacgaaaacg caggtagctt tcgacatcga
    32881 agaagaactg agtaatcgcg cggttggctc cggcatccac tttgcgtttc agattaagca
    32941 aatccgcctg agcgcttttt gcttccgggt gaacttccgg atacgccgcc acggagatat
    33001 cgaaatctgc cacttctttt aatagcgtca ccaggtcaga agcatacatt tctggcttac
    33061 cacttcccgg cggcagatcg ccacgcagcg ccacgatatg acgaataccg ttattccagt
    33121 agtcgcgtgc aatggtgcgc agctcgtcgg gcgtcgcatc aatgcaagta agatgcggtg
    33181 ccgcttccag accagtgcga tctttaatgc ctttaataat gctgtgcgta cggtcgcgct
    33241 cgccggagtt cgcgccatag gtcaccgata caaacttcgg tttcaggctg ctaaggcgat
    33301 cgatggagtt ccacagggtc tgctccattt cactggtacg cggcgggaaa aactcgaacg
    33361 aaacgttaat ctgcccctgg acttctgcca ggctctgatt cagggcatcc cgctggctgg
    33421 cgtgaaaaaa gctcatacct tacctcatca atcgcttgta gttatatgtt gtgttgtgca
    33481 catctatccg tttagacgtc cagatgtaaa gatgaaggaa aagccgaagg gcgtcaaccg
    33541 aaaaatgacc aatgatggtg aggaaaaatc agggaagatg aaaaaacttc aggatggtaa
    33601 gaaaaagaaa atccggtatg caaagcaaac cggactgcaa atataaacta accatgtact
    33661 tattgcttct tgccggattc gtcgtaaacg cttaatccgg cctaatccta tgataattca
    33721 atatatttca ttagaaacca taaacccgaa aacatgagta ccgggcatta ttaaatttct
    33781 gaaattacaa caactgtgcc agccggttga tatccgactg aatcgccccg gcggtgacgt
    33841 cgcgcccagc gccaggtccg cggatcacca gagggttatc gcgataccag cggctttcga
    33901 tggcaaagac gttatcgcac ggcagcagtg atgccaacgg atgatcttca cgcaccgctt
    33961 ccacgcctac acgcgcttta ccgttggcat cgaaacgcgc cacgtagcgc agcaccagcc
    34021 ccatttcgcg ggccgcttcc agccgttgca ccatctgctc gttcagttca tcgccatttt
    34081 caaagaaatg gtcgatgctg ccgccttcgc aatgagcagg caccagcgat tccacacgta
    34141 cctgatccgg ttcgatgttg taacctgctt cacgcgccag aatcaccagc ttgcgcatca
    34201 cgtctttgcc agagagatcg tcacgcgggt caggttcggt taagccctgc tgccacgcct
    34261 gatccaccag ctcggtaaac ggcacgctac cgtcgaattg caggaacagc caggagagcg
    34321 tgccggagaa gatcccgctg atcgacaaaa tagtatcgcc gctgtcgatc agatcgcgca
    34381 cggtgtggtt gatcggcaag cccgcaccga cggtggcatt gtacagccag tgacgcccgg
    34441 ttttttcgaa ggcgtcgtgg atctggcgat atttattgct gtcgctggct cccgccagtt
    34501 tgttggcgct gataacgtgg aaaccgtggc tggcgaaatc aagatactga tcagcaagct
    34561 gctggctggc ggtaacgtcc agcaccacta aatcatcata cggatgggcg cgcatccaca
    34621 ggaacaacga ctcttcatcc tgctcaaccg cttcatcgtt gaagaaggct aacgcgcggc
    34681 tggcgtccag cccgtcatag ctcaacaggc tgcggcggct gtccaccaca cctgccagca
    34741 caaactcaaa gccggtacgt gccgaaagcg tgctctgctc acgggcgaac agttccagcc
    34801 aacgggaacc gatattgccc ttaccgaaca ataccaggcc gatgcgtttt tctgcgcgga
    34861 agacggactg atgcagcccc tgaatcaggc tttcggtcgg gccggtgcgc agtactgcca
    34921 ccaggctgat gccgtcatcg gactgccagg taaattcgac cggctggcct ttcagttgct
    34981 gccagaagcg gtggcaatgc agcgggttac gggtgacgcc tgcaccgacc atcgccacca
    35041 gcgccagccc ctgacgcagg cgcagttcgc caggtaatcc cgcttcgtcg aggattttca
    35101 gcgcactgtc ggccacttct gaggtgtagc aaaattgcag caactggcga tcgttatgta
    35161 cgccaaccgc cagcgggcgt acctgcgcgc gtttcaggat ttggtcgatc tctttatgcg
    35221 ccagtttgaa atcctgactg gcgggcacct gaaactcaat caaacagaca tcatcgtggc
    35281 tggtgacaat acgcgcacca gtaccggagg ccagcacgcg ttcaatgcgc gtggaacctt
    35341 gatccggcgt gtagctacag cgcagttgca ggtcgatttc gctgccagaa accggctgta
    35401 aagtacgggc gtgaagaacg ggagccgcca ggcgcgccag ttcgctggcc tcatccagac
    35461 gcagcaacgg cagcaggcag gcatctttca ctttacgcgg gtcggcactg tataccccgg
    35521 cgacgtcgct ccagatggtt acgcgagaaa cacccgccag cgcaccgatt tgtgtcgcgg
    35581 aatagtcgga accgttacgc cccagcagca ccgtttcacc ggcgttgttg cggctgataa
    35641 atccggtcac caccagacgt ttgcccggat gttgcaccag cagctgttgc agcaacgggt
    35701 aagaaagccc ttcatcaacc tgcggttgtg cggcgcgttc agcgcgtaaa aactcgcggg
    35761 catcaagcca ggccgctggc agcccttgtt gattaagtac cgcagacatc aggcgcgccg
    35821 accacacttc accgtggccc accacttccg catacaccgc atcgttaata ccactgtcga
    35881 gcaacgccgc caggcgttcc agatcgctga caaaagcgct aatgaggcta tcggcttctt
    35941 cagcgggtag cagaccgcta atcagatcgc actgatagcg acgcagcgtt tgttgaacct
    36001 gatgcgcaga gagacgatcg gtctggctta gtttcaacca gttaatcaac tggttagtgg
    36061 tgctaccggc ggcggaaacc accatcatat cgtcaggctg agagtactcc gccataatgc
    36121 ccgcgacacg caaataacac ttcacatcag ccagactact gccaccaaat ttatgcagct
    36181 gacgaccttt cgcccctgcc tgcgcaatca cactcatttt gaccccttgt ttgcagcccg
    36241 gaagccattt tccaggtcgg caattaaatc ttcgccatct tcaataccgg tggagatacg
    36301 cagcagcgtc tcggagatcc cggcggcagc acgcgcttct ggtgccatgc ctgcatgtgt
    36361 catggttgcg gcgtgagaga ttaaactttc cactccccct aatgattccg ccagcgtaaa
    36421 caacgacagc ccgcccagga aacgacgcag cgtctgctca tcgccatcca gttcaaaact
    36481 caacattgcg ccaaagcctt tttgctggcg cgcggcaatt tcatgcccct gattttccgg
    36541 cagcgacggg tgatacagtt ttttcaccaa cggctgggtt tgcagatatt tcacaatcgc
    36601 ctgcgcgtta cgctgcgcca gctccatacg cggcaccagc gttcgcaacc cacgtagcag
    36661 cagatagctg tcaaacgcgc cgcccgtcac gccaatattg tttgcccacc aggccagttc
    36721 agtgacaacg tccgggtctt tagcaatcac cacgccggcc actacgtctg agtgaccgtt
    36781 cagatatttc gtgcatgaat gcaacaccag atcggcacct aatgccagcg gattttgtaa
    36841 tgccgggctt aagaaggtgt tatccaccac gctcaccgcc ccgacttccc ttgccagatg
    36901 gcagattttc gcaatatcca cgacgcgtaa caatggatta cttgggcttt ctaccagtac
    36961 cagtttgggt ttttctgcca gcgctgcccg taatgcctgt tcatcgcctt gatcaacaaa
    37021 caacacgcga tagcaaccac gtttcgccag gctgtcgaac aggcgatagc taccgccgta
    37081 gcagtcgtgc ggcgcaacca gcaaatcgcc aggtttcaaa aagacggtgg ttaccagatg
    37141 gatggctgac atgccggtat tggtgagcac tgcaccggca ccaccttcca gttctgccag
    37201 cgcacgctga accacatcgc gcgttgggtt gccgcgacgc gagtaatcat gcgcgcgcgg
    37261 ttcattaaat ccggtaaagt tataggtgct ggaaagatgg atcggtggga caacgcaacc
    37321 atactgttcg tcgtcattta acccgctacg cactgcgatg gtggcctgtt tacgcgtcat
    37381 gtgatgaagt tccctgggct ttgtcggtga aatgtcaggc accagagtaa acattgtgtt
    37441 aatggacgtc aatacatctg gacatctaaa cttctttgcg tatagattga gcaaatccca
    37501 aatagccgtt aaaattatat gcattatcac gccgacaggt gcattacacg atgtcacggt
    37561 aacgcctgta cggtaaacta tgcgggttta cggtcagtac ccacatcaac tgtgtggtct
    37621 ggtctcaatt tattgacgaa gaggattaag tatctcatgg ctgaatggag cggcgaatat
    37681 atcagcccat acgctgagca cggcaagaag agtgaacaag tcaaaaagat tacggtttcc
    37741 attcctctta aggtgttaaa aatcctcacc gatgaacgca cgcgtcgtca ggtgaacaac
    37801 ctgcgtcacg ctaccaacag cgagctgctg tgcgaagcgt ttctgcatgc ctttaccggg
    37861 caacctttgc cggatgatgc cgatctgcgt aaagagcgca gcgacgaaat cccggaagcg
    37921 gcaaaagaga tcatgcgtga gatggggatt aacccggaga cgtgggaata ctaaagcaaa
    37981 aaagagcggc gcggagtgga atcgcctgat gcgctacgct tatcaggcct acgtcatatt
    38041 gcaatttatt gaatttgcac gaacttgtag gccggataag gcgttcacgc cgcatccggc
    38101 ataaacaacg agcacgttgt ctgcgaccca ccgcttttta tacatggacg tttaactatg
    38161 aaaaacaggc tgctgatcct cagcctgctg gtttctgtac ctgcctttgc ctggcagcca
    38221 caaaccggcg acatcatctt tcagatctct cgctcatcgc aaagtaaagc gatccaactg
    38281 gcgacccatt ccgattatag ccacaccggt atgctggtga tacgcaacaa aaagccctac
    38341 gtttttgaag cggtcggccc ggtgaaatac accccgctca agcagtggat cgcccatggt
    38401 gaaaagggca aatacgttgt tcgccgcgtt gaaggcggac tgagtgttga acaacagcaa
    38461 aaactggcgc aaacggcaaa acgttatctc ggtaaaccgt atgatttcag cttctcatgg
    38521 agcgacgatc gccagtactg ttcggaagtg gtgtggaagg tttaccagaa cgcgctggga
    38581 atgcgcgtgg gcgagcagca gaagttgaaa gagttcgatc tcagcaaccc gctggtccag
    38641 gctaagctca aagaacgcta cggcaaaaat attccgcttg aggaaacggt agtttcacca
    38701 caagccgttt tcgacgcgcc acaactcacc acagtcgcca aagagtggcc gctgttttcg
    38761 tggtaatgga tgatgaaaaa aataattccc gccgtagtct gtttgttgct ttgtagttgc
    38821 tcgacctttc cgcaagctgt cgctccggta aacaatgact ttgcaggtca gcataatatt
    38881 tatattgtca gtcacggctg gcataccgga attgtggtgc ccgccgcaat cgttaatcgt
    38941 gttttaccgc aactcgacgc gagatttgcg caaccaaaat ggtatgaaat cggctggggc
    39001 gataaaggct tttatcaggc acaggaaatt accagtcgcc tgacattgca ggcgatgttc
    39061 tggtctactg gcgcagtaat gcatgtggtg gcgttctctg ccccacctga acgctatttt
    39121 cctggcagtg aagttaagcc actgacaatt aacaacggtc aacttgcgac cttaatgcta
    39181 tttattggcc gcagtttttc ccgtagcgag gcgggaaatg ttattccact caaaaaaggc
    39241 atttatggtg acagccagtt ttatgccgcc aatggtcgct atgggatttt aaatacctgt
    39301 aataaatgga cggcaaaagg gttacaaagc gcggggatag agattgaacc ggctttaaaa
    39361 ttgacggcgg ggagtgtgat aagcgcaatg ccttaacgat tgtcaggcac aaaaaaagcg
    39421 ccgtgcggcg cttttttcgg aaatccggtc ttatttgctg cccgggatgt tgaaacgctt
    39481 gttgaagcgg tcaacacggc caccggtagc aacatcacgc tgtttgccag tgaagaatgg
    39541 gtggcacttg ctgcacacgt cgaggttcag gtcatgacca acggtggagc ggattttcat
    39601 tacgttaccg caagagcagc tagcagtaat ttcttcgtat ttcgggtgaa tatctttttt
    39661 catgggaaaa cctcggttta aggccgcgtc gctcttccag ccctaacgcc agacaccacg
    39721 cgatgttaaa agtatagctt caatacgatc atttcgtacg aaggcgcgaa atcatacaga
    39781 aattaaccag cgtatgcaaa ctgatccgca ctcttctacg gcaatgtgta tactaaccca
    39841 ccgaatttca agtcaggatg atgctatgcc cgttgcccac gttgccttgc ccgttccgct
    39901 tcctcgtacc tttgactatc tgctgccaga aggcatgacg gttaaagccg ggtgtcgcgt
    39961 gcgcgtgccg tttggcaaac agcaggagcg catcggggtt gtggtgtcag ttagcgatgt
    40021 cagtgagctg ccgcttaacg agctgaaagc ggtagtggaa gtgctggacg ttgagccggt
    40081 atttactcac tccgtctggc ggttactgct ctgggcggca gattactatc atcatccgat
    40141 tggcgatgtg ctgtttcatg ccttgccgat tttactgcgc caggggcggc ctgcggcgaa
    40201 cgcgccgatg tggtactggt ttgccactga acaaggccag gcggtggatc tgaacagcct
    40261 gaaacgctcc cccaagcaac aacaggcgct ggcggcgtta cggcaaggca aaatctggcg
    40321 cgaccaggtc gccacgctcg aatttaatga tgccgcgttg caggcgctac gcaaaaaagg
    40381 tctgtgtgat ttagcaagtg aaacaccaga gtttagcgac tggcgaacga actatgccgt
    40441 ttctggtgag cggttgcgat tgaataccga acaggccacc gccgttggcg caattcatag
    40501 cgcggcagat actttttctg cctggctgct ggcgggcgtt accggttccg gtaaaacgga
    40561 ggtttatctc agcgtactgg aaaacgtgct cgctcagggc aaacaggcgc tggtgatggt
    40621 gccggaaatc ggcctgacac cgcaaactat cgcccgtttt cgtgaacgtt ttaatgcccc
    40681 cgtggaagtt ctgcattccg gcctgaacga cagcgagcgt ctttcggcgt ggctgaaagc
    40741 gaaaaatggt gaggcggcga ttgtgatcgg cacccgctcc gcgctgttta cgccgtttaa
    40801 aaatctcggc gtgattgtca ttgatgaaga gcacgacagc tcctacaagc agcaggaagg
    40861 ctggcgctat catgcccgcg acctggcggt gtatcgtgcg cacagcgagc aaatcccgat
    40921 tattcttggc tccgcaacgc ccgcgctgga aacgttatgc aacgtccagc agaaaaaata
    40981 ccgcctgctg cgcctgaccc gtcgggcagg gaatgcgcgt ccggcaattc aacatgtgct
    41041 ggatttaaaa ggtcagaagg tgcaggcagg tctggctccg gcgttaatca ctcgtatgcg
    41101 ccagcattta caggctgata accaggtcat tctctttctt aaccgccgtg gctttgcgcc
    41161 tgcactgctg tgccacgact gtggctggat tgccgaatgc ccacgttgcg atcactacta
    41221 cacgctgcat caggcgcagc accatctgcg ctgccaccac tgtgacagtc agcgtccggt
    41281 gccgcgccag tgcccttcct gcggttccac gcacctggtc cccgtggggc tgggcaccga
    41341 acagcttgaa cagacgctcg cgccgttgtt ccccggcgtg cccatttctc gtatcgaccg
    41401 cgataccacc agccgcaaag gggcgctgga acagcaactg gcagaagtac atcgcggcgg
    41461 cgcgcggatt ttgattggta cacaaatgct ggcgaaaggt caccatttcc cggatgtgac
    41521 gctggttgca ttactggacg tggacggcgc gctgttttct gccgattttc gctcggcaga
    41581 gcgtttcgct cagctttaca cccaggtcgc cggtcgtgcc gggcgtgcgg gtaaacaggg
    41641 cgaagtggtg ctgcaaacgc accatccgga acatcctctg ttgcaaacgt tgctctataa
    41701 aggctacgac gcctttgccg aacaggcgct ggctgagcgg cgaatgatgc agctaccgcc
    41761 gtggaccagc catgtgattg tgcgtgcgga agatcataac aatcagcacg cgccattgtt
    41821 cctgcaacaa ctgcgtaatc tgatcctctc cagcccactg gcagacgaga aactgtgggt
    41881 tctcggtccg gttccggctc tggcacctaa acgtggcggt cgctggcgct ggcagatatt
    41941 gttgcagcac ccttcccgcg tgcgcttgca acacatcatt aacggtacgc tggcgctcat
    42001 caatacaata ccggattccc gtaaggtgaa atgggtgctg gatgttgatc cgattgaggg
    42061 ttaaaccgct cacgatgcga ggcggatcga aaaattcaat attcatcaca cttttcatga
    42121 aaattctgta accgttttca cgcgctatct gctaaaaatg ttgccgatgt gaagtaaaca
    42181 tggatgtagt acgcctgacg tgccaggcga ggagtgagtg tgaaagcgaa gaagcaggaa
    42241 actgccgcga ccatgaaaga cgttgccctc aaggcaaaag tctctacagc gaccgtctcc
    42301 cgagcattaa tgaatcccga taaagtctca caggccaccc gtaatcgggt tgaaaaagcg
    42361 gcccgggaag tgggttattt accgcagcct atggggcgca acgtcaagcg taatgaatcc
    42421 cgcaccattc tggtgattgt cccggatatc tgcgatccct tctttagcga aattattcgc
    42481 ggtatcgaag ttacggcggc aaatcacgga tatctggtgc tgattggcga ctgtgcgcat
    42541 caaaatcagc aggaaaaaac ctttatcgat ttgatcatca ccaagcaaat tgatggcatg
    42601 ttgttgctgg gttcaaggct gccgtttgat gccagcattg aggaacagcg taatctgccg
    42661 ccgatggtga tggcgaacga atttgcaccg gagctggagc tgcctacagt tcatatcgac
    42721 aatctgaccg ccgcatttga tgcagtaaat tatttatatg agcaagggca taaacggatt
    42781 ggctgtatag ccggtcccga agagatgccg ctgtgtcact accgcctgca aggctatgtt
    42841 caggcgctgc gtcgctgcgg cattatggtt gatccgcaat acatcgcccg tggcgacttc
    42901 accttcgaag ccggaagcaa agcgatgcag cagctgcttg atcttccaca accgcctact
    42961 gctgtcttct gccatagcga tgtgatggcg ctcggcgcac tttctcaggc aaaacgccag
    43021 gggctgaaag tcccggaaga cctttccata atcggttttg ataacatcga cctgacgcaa
    43081 ttttgtgatc cgccgctgac aaccatcgcg cagccgcgtt acgaaatcgg tcgggaagct
    43141 atgctgttat tgcttgatca aatgcagggg caacacgttg gcagtggctc tcgtttaatg
    43201 gactgcgaac ttatcatccg gggatcaaca cgcgcgttac cttaaagtaa acctttaaga
    43261 ctgccgtgac tggtcaaagg cccgccgctt aagtaacatg gcgggctgac gaacgaataa
    43321 atacagcgaa acgatagtgg cacaacgaga ttatgtacgc cgcagccaac cggcaccttc
    43381 gcggcgaaaa aagagcacct cacggaaaaa gcaacgaaat ctgcctgcgg tttctcccgc
    43441 tatggtcgct attgctgccg ccgttcttgt gacctttatc ggtggtctgt acttcattac
    43501 gcatcacaag aaagaagagt ccgagacgct gcaaagccag aaagtgaccg gaaacggact
    43561 accaccaaaa ccagaagaac gctggcgcta cattaaagag ctggaaagtc gccagccggg
    43621 agtgcgtgcg cccacagaac cttctgccgg tggtgaagtg aaaacgccgg agcaactgac
    43681 accagaacaa cgtcagcttc ttgaacaaat gcaggctgat atgcgccagc agccaacgca
    43741 gctggttgaa gttccgtgga atgaacagac gccggaacag cgtcagcaaa cgctacagcg
    43801 ccaacgtcag gcgcagcaat tagcggaaca gcaacgcctg gctcagcagt cgcgtacgac
    43861 tgaacaaagc tggcagcagc agacgcgtac gtcgcaagcc gcaccggtac aggcgcaacc
    43921 gcgccagtct aaaccggctt cttctcagca gccgtaccag gatctgctgc aaactcctgc
    43981 gcacacgact gcgcagtcga agccgcagca agctgcgcca gttgctcgtg ccgctgacgc
    44041 gccaaaaccg acggcggaga aaaaagacga acgccgctgg atggtgcagt gcggttcgtt
    44101 cagaggcgcg gaacaggcag agacggtacg tgctcagctg gcgttcgaag gctttgactc
    44161 gaaaatcacc accaacaatg gctggaatcg tgtggtcatt ggcccggtga aaggcaaaga
    44221 gaacgcagac agcaccctca atcggttgaa gatggcgggt catacaaact gcattcggct
    44281 cgccgccggg ggttgaaacc ctcaaaatcc cccccatcta taattgcatt atgccccgta
    44341 cttttgtacg gggtttgtac tctgtattcg taaccaaggg gtcagctcgt gacaactata
    44401 gtaagcgtac gccgtaacgg ccatgtggtc atcgctggtg atggtcaggc cacgttgggc
    44461 aataccgtaa tgaaaggcaa cgtgaaaaag gtccgccgtc tgtacaacga caaagtcatc
    44521 gcgggctttg cgggcggtac tgcggatgct tttacgctgt tcgaactgtt tgaacgtaaa
    44581 ctggaaatgc atcagggcca tctggtcaaa gccgccgttg agctggcaaa agactggcgt
    44641 accgatcgca tgctgcgcaa acttgaagca ctgctggcag tcgcggatga aactgcatcg
    44701 cttatcatca ccggtaacgg tgacgtggtg cagccagaaa acgatcttat tgctatcggc
    44761 tccggcggcc cttacgccca ggctgcggcg cgcgcgctgt tagaaaacac tgaacttagc
    44821 gcccgtgaaa ttgctgaaaa ggcgttggat attgcaggcg acatttgcat ctataccaac
    44881 catttccaca ccatcgaaga attaagctac aaagcgtaag gatctcccat gtctgaaatg
    44941 accccacgcg aaatcgtcag cgaactggat aagcacatca tcggccagga caacgccaag
    45001 cgttctgtgg cgattgctct gcgtaaccgc tggcgtcgca tgcagctcaa cgaagagctg
    45061 cgccatgaag tgaccccgaa aaatatcctg atgatcggcc cgaccggtgt cggtaaaact
    45121 gaaatcgccc gtcgtctggc taagctggcg aatgcgccgt tcatcaaagt tgaagcgacc
    45181 aaattcaccg aagtgggcta cgtcggtaag gaagtggatt ctattattcg cgatctgacc
    45241 gatgccgccg tgaaaatggt acgcgtccag gctatcgaga aaaaccgtta tcgcgctgaa
    45301 gaactggcag aagaacgtat tctcgacgtg ctgatcccac ctgctaaaaa caactgggga
    45361 cagaccgaac agcagcagga accgtccgct gctcgtcagg cattccgcaa aaaactgcgt
    45421 gaaggccagc ttgatgacaa agaaatcgag atcgatcttg ccgcagcacc gatgggcgtt
    45481 gaaattatgg ctcctccggg catggaagag atgaccagcc agctgcagtc catgttccag
    45541 aacctgggcg gccagaagca aaaagcgcgt aagctgaaaa tcaaagacgc catgaagctg
    45601 ctgattgaag aagaagcggc gaaactggtg aacccggaag agctgaagca agacgctatc
    45661 gacgctgttg agcagcacgg gatcgtgttt atcgacgaaa tcgacaaaat ctgtaagcgc
    45721 ggcgagtctt ccggtccgga tgtttctcgt gaaggcgttc agcgtgacct gctgccgctg
    45781 gtagaaggtt gcaccgtttc caccaaacac gggatggtca aaactgacca cattctgttt
    45841 atcgcttctg gcgcgttcca gattgcgaaa ccgtctgacc tgatcccgga actgcaaggt
    45901 cgtctgccaa tccgcgttga actgcaggcg ctgaccacca gcgacttcga gcgtattctg
    45961 accgagccga atgcctctat caccgtgcag tacaaagcac tgatggcgac tgaaggcgta
    46021 aatatcgagt ttaccgactc cggtattaaa cgcatcgcgg aagcggcatg gcaggtgaac
    46081 gaatctaccg aaaacatcgg tgctcgtcgt ttacacactg ttctggagcg tttaatggaa
    46141 gagatttcct acgacgccag cgatttaagc ggtcaaaata tcactattga cgcagattat
    46201 gtgagcaaac atctggatgc gttggtggca gatgaagatc tgagccgttt tatcctataa
    46261 tcgcgttcaa tcattttcat cattgtttga tggggctgaa aggccccatt tttattggcg
    46321 cgtattatga ctgaacaaca aattagccga actcaggcgt ggctggaaag tttacgacct
    46381 aaaaccctcc ccctcgcctt tgctgcaatt atcgtcggga cagcgctggc atggtggcaa
    46441 ggtcacttcg atccgctggt cgccctgctg gcactaatta ccgccgggct attacagatc
    46501 ctttctaacc tcgccaatga ttacggcgat gcggtaaaag gcagcgataa acctgaccgc
    46561 attgggccgc tacgcggcat gcaaaaaggg gtcattaccc agcaagagat gaaacgggcg
    46621 ctcattatta ccgtcgtgct catctgtctc tccgggctgg cactggttgc agtggcatgc
    46681 catacgctgg ccgattttgt cggtttcctg attcttggcg ggttgtcgat cattgccgct
    46741 atcacctaca ccgtgggcaa tcgtccttat ggttatatcg gtctgggtga tatttccgta
    46801 ctggttttct ttggctggtt gagtgtcatg gggagctggt atttacaggc tcatacattg
    46861 attccggcac tgatccttcc ggcgaccgca tgcggcctgc tggcaacggc agtactgaat
    46921 attaataacc tgcgtgatat caatagcgac cgcgaaaatg gcaaaaacac gctggtggtg
    46981 cgcttaggtg aagtgaacgc gcgtcgttat catgcctgcc tgctgatggg ctcgctggtg
    47041 tgtctggcgc tgtttaatct cttttcgctg catagcctgt ggggctggct gttcctgctg
    47101 gcggcaccat tactggtgaa gcaagcccgt tatgtgatgc gggaaatgga cccggtggcg
    47161 atgcgaccaa tgctggaacg tactgtcaag ggagcgttac tgactaacct gctgtttgtt
    47221 ttagggatat tcctaagcca gtgggcagca taactgacaa atatcaatta acaattgatg
    47281 attttgccaa cagcccacat agcgcgatat actgaaaatt ctcgcagcaa ctgaatgtta
    47341 agcctatgaa atacgatact tccgagcttt gtgacatcta tcaagaagat gttaacgtcg
    47401 tggaaccgct gttctccaac tttggcggac gggcgtcgtt tggcggacaa ataatcacgg
    47461 taaaatgttt cgaggacaac gggttgctgt acgatctgct cgaacagaat ggccgtggtc
    47521 gtgttcttgt cgtcgatggc ggtggttctg ttcgtcgcgc actggtcgat gctgaactgg
    47581 cgcgtctggc agtacaaaat gaatgggaag gtctggtcat ttacggcgcg gtgcgtcagg
    47641 tagatgacct ggaagagttg gatatcggca tccaggcgat ggcggcaatt ccggttggtg
    47701 ccgctggcga aggcattggc gaaagcgatg tccgcgtcaa ttttggcggt gtcaccttct
    47761 tctccggcga ccatctttat gccgacaata ccgggattat tctttcagaa gatccgctgg
    47821 atattgaatg ataagaaagg caccgcaagg tgcctttttt ctgcgttacc tgttggccta
    47881 cacagtaaag aaattacgcg gaagatgaag cgtaatcaga cctcttccat gcgacccagc
    47941 agggcctgca gacgttcctg ccagccgttc tgctgttctt tcagatggtt gttctcacgc
    48001 tccagctctt cgcgctgatg ctgggcattt tgaacttcct gcgacagtga gttgtttttt
    48061 tctttcagct cttcgatttc catctgcaac agagtgatgg tatcaatcgc ctgctgtact
    48121 tttgcttcca gtttctcaaa cacttctaat gacattgtca tacctctcct gaattgcaag
    48181 gcgttgatgg ataaaaatcc tcgtcccgat taccggtgac gccttaataa atacgagcgc
    48241 actttagtta gctccgattg tatgaagccg cgccatcgct gtccagcggc acgccttgca
    48301 gattacggtt tgccacactt ttcatccttc tcctggtgac ataatccaca tcaatcgaaa
    48361 atgttaataa atttgttgcg cgaatgatct aacaaacatg catcatgtac aatcagatgg
    48421 aataaatggc gcgataacgc tcattttatg acgaggcaca cacattttaa gttcgatatt
    48481 tctcgttttt gctcgttaac gataagttta cagcatgcct acaagcatcg tggaggtccg
    48541 tgactttcac gcatacaaca aacattaact cttcaggatc cgattatgag tcaaacatca
    48601 accttgaaag gccagtgcat tgctgaattc ctcggtaccg ggttgttgat tttcttcggt
    48661 gtgggttgcg ttgcagcact aaaagtcgct ggtgcgtctt ttggtcagtg ggaaatcagt
    48721 gtcatttggg gactgggggt ggcaatggcc atctacctga ccgcaggggt ttccggcgcg
    48781 catcttaatc ccgctgttac cattgcattg tggctgtttg cctgtttcga caagcgcaaa
    48841 gttattcctt ttatcgtttc acaagttgcc ggcgctttct gtgctgcggc tttagtttac
    48901 gggctttact acaatttatt tttcgacttc gagcagactc atcacattgt tcgcggcagc
    48961 gttgaaagtg ttgatctggc tggcactttc tctacttacc ctaatcctca tatcaatttt
    49021 gtgcaggctt tcgcagttga gatggtgatt accgctattc tgatggggct gatcctggcg
    49081 ttaacggacg atggcaacgg tgtaccacgc ggccctttgg ctcccttgct gattggtcta
    49141 ctgattgcgg tcattggcgc atctatgggc ccattgacag gttttgccat gaacccagcg
    49201 cgtgacttcg gtccgaaagt ctttgcctgg ctggcgggct ggggcaatgt cgcctttacc
    49261 ggcggcagag acattcctta cttcctggtg ccgcttttcg gccctatcgt tggcgcgatt
    49321 gtaggtgcat ttgcctaccg caaactgatt ggtcgccatt tgccttgcga tatctgtgtt
    49381 gtggaagaaa aggaaaccac aactccttca gaacaaaaag cttcgctgta atatgactac
    49441 gggacaatta aacatgactg aaaaaaaata tatcgttgcg ctcgaccagg gcaccaccag
    49501 ctcccgcgcg gtcgtaatgg atcacgatgc caatatcatt agcgtgtcgc agcgcgaatt
    49561 tgagcaaatc tacccaaaac caggttgggt agaacacgac ccaatggaaa tctgggccac
    49621 ccaaagctcc acgctggtag aagtgctggc gaaagccgat atcagttccg atcaaattgc
    49681 agctatcggt attacgaacc agcgtgaaac cactattgtc tgggaaaaag aaaccggcaa
    49741 gcctatctat aacgccattg tctggcagtg ccgtcgtacc gcagaaatct gcgagcattt
    49801 aaaacgtgac ggtttagaag attatatccg cagcaatacc ggtctggtga ttgacccgta
    49861 cttttctggc accaaagtga agtggatcct cgaccatgtg gaaggctctc gcgagcgtgc
    49921 acgtcgtggt gaattgctgt ttggtacggt tgatacgtgg cttatctgga aaatgactca
    49981 gggccgtgtc catgtgaccg attacaccaa cgcctctcgt accatgttgt tcaacatcca
    50041 taccctggac tgggacgaca aaatgctgga agtgctggat attccgcgcg agatgctgcc
    50101 agaagtgcgt cgttcttccg aagtatacgg tcagactaac attggcggca aaggcggcac
    50161 gcgtattcca atctccggga tcgccggtga ccagcaggcc gcgctgtttg gtcagttgtg
    50221 cgtgaaagaa gggatggcga agaacaccta tggcactggc tgctttatgc tgatgaacac
    50281 tggcgagaaa gcggtgaaat cagaaaacgg cctgctgacc accatcgcct gcggcccgac
    50341 tggcgaagtg aactatgcgt tggaaggtgc ggtgtttatg gcaggcgcat ccattcagtg
    50401 gctgcgcgat gaaatgaagt tgattaacga cgcctacgat tccgaatatt tcgccaccaa
    50461 agtgcaaaac accaatggtg tgtatgtggt tccggcattt accgggctgg gtgcgccgta
    50521 ctgggacccg tatgcgcgcg gggcgatttt cggtctgact cgtggggtga acgctaacca
    50581 cattatacgc gcgacgctgg agtctattgc ttatcagacg cgtgacgtgc tggaagcgat
    50641 gcaggccgac tctggtatcc gtctgcacgc cctgcgcgtg gatggtggcg cagtagcaaa
    50701 caatttcctg atgcagttcc agtccgatat tctcggcacc cgcgttgagc gcccggaagt
    50761 gcgcgaagtc accgcattgg gtgcggccta tctcgcaggc ctggcggttg gcttctggca
    50821 gaacctcgac gagctgcaag agaaagcggt gattgagcgc gagttccgtc caggcatcga
    50881 aaccactgag cgtaattacc gttacgcagg ctggaaaaaa gcggttaaac gcgcgatggc
    50941 gtgggaagaa cacgacgaat aatgtaaatg ccgaatgaag cgtttatgcc gcatccggta
    51001 gtcccgaaac gtgcgggggc aaccccgcac acatcaataa tccctccctt cccctgtgct
    51061 acacttcgcg ccattcctta ctgcttagag tttgctatga gacgagaact tgccatcgaa
    51121 ttttcccgcg tcaccgaatc agcggcgctg gctggctaca aatggttagg acgcggcgat
    51181 aaaaacaccg cggacggcgc ggcggtaaac gccatgcgta ttatgctcaa ccaggtcaac
    51241 attgacggca ccatcgtcat tggtgaaggt gaaatcgacg aagcaccgat gctctacatt
    51301 ggtgaaaaag tcggtactgg tcgcggcgac gcggtagata ttgctgttga tccgattgaa
    51361 ggcacgcgca tgacggcgat gggccaggct aacgcgctgg cggtgctggc agtaggcgat
    51421 aaaggctgct tcctcaatgc gccggatatg tatatggaga agctgattgt cgggccggga
    51481 gccaaaggca ccattgatct gaacctgccg ctggcggata acctgcgcaa tgtagcggcg
    51541 gcgctcggca aaccgttgag cgaactgacg gtaacgattc tggctaaacc acgccacgat
    51601 gccgttatcg ctgaaatgca gcaactcggc gtacgcgtat ttgctattcc ggacggcgac
    51661 gttgcggcct caattctcac ctgtatgcca gacagcgaag ttgacgtgct gtacggtatt
    51721 ggtggcgcgc cggaaggcgt agtttctgcg gcggtgatcc gcgcattaga tggcgacatg
    51781 aacggtcgtc tgctggcgcg tcatgacgtc aaaggcgaca acgaagagaa tcgtcgcatt
    51841 ggcgagcagg agctggcacg ctgcaaagcg atgggcatcg aagccggtaa agtattgcgc
    51901 ctgggcgata tggcgcgcag cgataacgtc atcttctctg ccaccggtat taccaaaggc
    51961 gatctgctgg aaggcattag ccgcaaaggc aatatcgcga ctaccgaaac gctgctgatc
    52021 cgcggcaagt cacgcaccat tcgccgcatt cagtccatcc actatctgga tcgcaaagac
    52081 ccggaaatgc aggtgcacat cctctgattg atttgatcga ttgagccttc cagtccttcg
    52141 ggactggaat ttttttgttc ggagaacgaa gataaggcaa gtcaatcaaa acaggagaaa
    52201 aacatggctg attgggtaac aggcaaagtc actaaagtgc agaactggac cgacgccctg
    52261 tttagtctca ccgttcacgc ccccgtgctt ccgtttaccg ccgggcaatt taccaagctt
    52321 ggccttgaaa tcgacggcga acgcgtccag cgcgcctact cctatgtaaa ctcgcccgat
    52381 aatcccgatc tggagtttta cctggtcacc gtccccgatg gcaaattaag cccacgactg
    52441 gcggcactga aaccaggcga tgaagtgcag gtggttagcg aagcggcagg attctttgtg
    52501 ctcgatgaag tgccgcactg cgaaacgcta tggatgctgg caaccggtac agcgattggc
    52561 ccttatttat cgattctgca actaggtaaa gatttagatc gcttcaaaaa tctggtcctg
    52621 gtgcacgccg cacgttatgc cgccgactta agctatttgc cactgatgca ggaactggaa
    52681 aaacgctacg aaggaaaact gcgcattcag acggtggtca gtcgggaaac ggcagcgggg
    52741 tcgctcaccg gacggatacc ggcattaatt gaaagtgggg aactggaaag cacgattggc
    52801 ctgccgatga ataaagaaac cagccatgtg atgctgtgcg gcaatccaca gatggtgcgc
    52861 gatacacaac agttgctgaa agagacccgg cagatgacga aacatttacg tcgccgaccg
    52921 ggccatatga cagcggagca ttactggtaa gcggttactt atcgataaac ggcacgatga
    52981 gcaaatccgc actcatctta ttgatcatcc cgcgatatgc cggcatcaaa cggttgataa
    53041 atgagtgatg atgaccacag acaaggaggt cgcactgctc tttttgcata atttccagca
    53101 gtgtttccgg catttctccg cgttcaatac gcagttttgt cttcggccat tgaatatttt
    53161 tcgtcagttt atacagcttg ttatccgact tattcttcaa caattgaaga atatcttctg
    53221 ttgcagggaa gtagataccc ggatacaact cgcttaagcc atcatcaata tgaattaacg
    53281 tcaggtgagc gtcattatgt ctggcgagct ccagggcttt attcaccagt aaggcatctt
    53341 cttcattccc ggaaattgcc acgccaatgt gtttataagc catgtttaac tccttctaaa
    53401 gcaactccat caagctaacg aacgcagtga taactcaaaa ataatcatct cggcctggca
    53461 tgagaaagtg aaggccgcat caagctcaac ttgcccctcc agatccttaa tcttataggc
    53521 cacgctgccc ccttcatctg cagaggatgt agcggcggca agctctttca gtttggtcat
    53581 aaaagactct gcttcttcgc gggtggcaaa aaagcgcgaa aacttactgg tacagttgtc
    53641 gttatcaatc accgtaccga tatcaatcgc acatccttta gtactgcatt tatctacgac
    53701 atctttcata gggcacctct gtatattcgc ctctctgtcg attacgactt aagaataatg
    53761 cgccgttaag ggaagaacaa acaagtaatg atcttatgga ttaccatttt ttttgatatt
    53821 gcttaaggtg aagagttcag aaggattttc cgggcattgt cattttcatc gtggaaataa
    53881 caatcccgga aaaaaatagc agtaagagat atattaaagg ttaattactt gatttattgt
    53941 cggctttata cttcacatcc tgggtatctt taccatattt attttcgcct tgtgtgccaa
    54001 caaacgcacc aagatcgata agcatcatca ccagaatcaa cgtcgggaca aaacgcccca
    54061 ccgcccattg ccagacaccc ggtaaaatcg cccagttacc cgccagcagc atccacgcca
    54121 caatcatcag aaatgcccat gcgccggaac gcccgcgatc atgcaagcgc ttaacggtta
    54181 ctgccgctgt tggccagagc aagcacacaa ggcaaaacgc cgcggtctga atatcgagta
    54241 aatttttacc cgccagtgag aaaagcacca gcatgcctgc gaaccacagg cctatccaaa
    54301 tccagaaatc acggcgtcca atacgccctt taaatgagaa taaccattgc tgtatggtca
    54361 tgtaagttcc ttgatggttg tcttttccag gattctaccc ttttgacaag ggcgacagat
    54421 atcgttttaa tcggagccag tcataacaaa aggtactgtc aatgaagcca gggtgtacgc
    54481 tgttttttct cttatgttct gcattaaccg ttacaacagc ggcgcatgca caaacaccag
    54541 atacggcaac gaccgcgcct tatctgctgg ctggagcccc tactttcgat ctctccatca
    54601 gccagtttcg agaagacttt aacagccaga atcccagcct gccactgaac gaatttcgtg
    54661 ccatcgacag cagtcccgac aaagccaatc tcactcgtgc tgcaagtaaa attaatgaga
    54721 acttgtatgc ttctacagcg ctggagcgcg gtaccttaaa aatcaaaagc attcaaatga
    54781 cctggctacc catccagggg ccagagcaaa aagccgcgaa agcgaaagct caggaataca
    54841 tggcagcggt gatccgcaca ctcaccccat taatgaccaa aacacaaagc cagaaaaaac
    54901 tgcagtcgct actaacggcg gggaaaaaca aacgttatta caccgagaca gaaggtgcac
    54961 tgcgttatgt tgtcgcggac aacggcgaaa aggggctgac cttcgctgtt gaaccgatta
    55021 agctggcgct atctgaatcg cttgaaggtt tgaataaatg acaaaaagca aagcctttgt
    55081 gccgatgaat ctctatactg tttcacagac ctgctgccct gcggggcggc catcttcctt
    55141 tattcgctta taagcgtgga gaattaaaat gcgacatcct ttagtgatgg gtaactggaa
    55201 actgaacggc agccgccaca tggttcacga gctggtttct aacctgcgta aagagctggc
    55261 aggtgttgct ggctgtgcgg ttgcaatcgc accaccggaa atgtacatcg atatggcgaa
    55321 gcgcgaagct gaaggcagcc acatcatgct gggtgcgcaa aacgtggacc tgaacctgtc
    55381 cggcgcattc accggtgaaa cttctgccgc tatgctgaaa gacatcggcg ctcagtacat
    55441 catcatcggc cactctgaac gtcgtactta ccacaaagag tctgacgaac tgatcgcgaa
    55501 aaaattcgcg gtgctgaaag agcagggcct gactccggtt ctgtgcatcg gtgaaaccga
    55561 agctgaaaac gaagcgggca aaactgaaga agtttgcgca cgtcagatcg acgcggtact
    55621 gaaaactcag ggtgctgcgg cattcgaagg tgcggttatc gcttacgaac ctgtatgggc
    55681 aatcggtact ggcaaatctg caactccggc tcaggcacag gctgttcaca aattcatccg
    55741 tgaccacatc gctaaagttg acgctaacat cgctgaacaa gtgatcattc agtacggcgg
    55801 ctctgtaaac gcgtctaacg ctgcagaact gtttgctcag ccagatatcg acggcgcgct
    55861 ggttggcggt gcttctctga aagctgacgc tttcgcagtg atcgttaaag ctgcagaagc
    55921 ggctaaacag gcttaagtct gacaggtgcc ggatttcata tccggcactt actttcctta
    55981 actcttcgcc ttaacgcaaa atctcacact gatgatcctg aatttcctcg gctgaagcac
    56041 ggttaagcgt cagtagattt cgttgtgtcg ccagcaatac aaatgagtta tcactctgcc
    56101 gtaccatcgc cagcccgtag cttcccatat gttcccgcgc ctcaggtact tcttctgcca
    56161 gcattataaa tgggctgcgt tgtaccagtt cgctttccgt tacccgacgc gccaggtatt
    56221 catgcccgcg caaaccacct ggcagtggca accagcggct gctgatgttc gccagattgt
    56281 tatccagttg ttcgcgcaca tcaggacgaa tacaagagat atgaatatga aaatggtttt
    56341 gcgtacgccc ggtgcgggag ttgatcgcca aagaaaccgc gcgatcggga accggctggc
    56401 cgtatttttt gctcataaaa tcacgcgcct gccaggccaa ccaaaagaag ttcggcgttg
    56461 aaggatcggt caacaaagga ctttcagtac cgttaatacg atacgttggc atcaacagat
    56521 attgcagtgg accattaaga tcttttaaaa ccacgtatcc ggcattgggt ttgacttccg
    56581 cacatggcga aggattttga ttttgctgct gattgggcaa acattcctca aggacaatct
    56641 tacgtaatgt atccgactct tcaccggtta atttccagta accaataccg gcagccacaa
    56701 cggcgataac tatcatcacc aaaaaaagaa gacccgcttt tttcatcttt ttttccctgt
    56761 acctcaaaga gatgcaaggg taacgcaaaa tcgtgacaaa taaaaaaccc ggtcgcgaga
    56821 aaccaccggg tttgataatt atcctgggga aatcagcgtt tgctgatctg atcgaacgta
    56881 ccgccgttag caaaatgctc tttttgcgct ttcgtccagc cgccgaactc ttcatcaatg
    56941 gtgaataact tcagctttgg aaacgcattt tcgtactttt tcgccacctc agcgtcgcgc
    57001 ggacggtagt agtttttcgc ggcaatttcc tgaccttctg gcgagtagag atatttcagg
    57061 taggcttccg ccacctcttt agtacctttt ttctcgacca ctttatcgac caccgacacg
    57121 gttggttccg cgaggataga ctcactcggc gtgacgattt cgaatttatc tttccccagt
    57181 tcattcgctg ccaacagagc ttcgttttcc caggcaatca gtacatcgcc aattccgcgc
    57241 tcgacaaaag tgttagtgga gccgcgcgcg ccagaatcca gaacttcgac gtttttatac
    57301 agtgcccgaa caaaatcctg tgcttttgct tgatcgttgt tgttgtgatg cagcgcgtag
    57361 ccccaggctg ccaagtaatt ccagcgcgcg ccaccagagc ttttcggatt aggcgtgatc
    57421 accgaaacac ccggtttaat cagatcgttc cagtcatgga tctgcttcgg atttccctta
    57481 cgtaccagga aaacaatggt ggaagtgtac ggtgcggagt tatccggcag acgtttgatc
    57541 cactctttat caatccgccc gcgttccgca attgcgtcca cgtcataggc cagagccagc
    57601 gtgacaacat cagcttcaat accgttgatt accgacgtcg cttgtttacc tgagccaccg
    57661 tgtgactgac gaatcaccac gttatcacca gtttgctgtt tccagtgggc gctgaatgcc
    57721 ttgttgtact gttcgtacaa ttcgcgcgtt ggatcatatg aaacgttaag aagctgaata
    57781 tcctttgcca taacgctggt tgccgccagc aaaaatgtta accctacgcc ccacttgttc
    57841 atcgcccgac tctcttatgt tgtgttgtga tgagcaaagc gtgccagaag gttaaccaaa
    57901 cattaaagaa taaaaaaaga ttggctataa cttgcgggta tatgttgggg gattaaaaag
    57961 gcggaagaaa tccgcctcat attgctgaca aagtgcgctt tgtccatgcc ggatgcggcg
    58021 cgaacgcctt atccggccta caaaagtttg caaattcaat aaattgcaga attcatgtag
    58081 gcctgataag cgaagcgcat caggcatttt tgcttctgtc atcggtttca ggctaaagga
    58141 atctgccttt ttccgaaatc attaatacag ttttttcgcg cagtccagcc agtcgccttt
    58201 gaacggacgc ttcatgtttt cgatagcgtc gatgatgtcg tggtgaacca actgttcgtt
    58261 ctggatacct acgcaacgac cgccgtaacc tgccagcagc agatcgatag cgtaagcgcc
    58321 catacgggaa gccagaatac ggtcgtaagg caccggagaa ccaccgcgct ggatgtggcc
    58381 cagcacagtt gcgcgggttt cacgaccggt ttctttctcg atgaaatgcg ccagttcgtc
    58441 aacatcacac atatgttcgg taatcgccac gatcgcgtgt tttttacctt tcgcgatacc
    58501 cgctttgatt tcgtttacca ggtcttcacg gctgaattca acttccggaa ccacaacgaa
    58561 ttcacagcca ccggcaatgg ccgcagccaa cgtcagatcg ccacaataac ggcccatcac
    58621 ttccaccacg gaaatacgct ggtgagaaga agaggtgtca cgcagacggt cgatcgcttc
    58681 tacaacggtg ctcagcgcag tgaagaaacc gatagtgtag tcagtgcctt tgatgtcgtt
    58741 gtcgatagtg cccggcagac cgatgcacgg gaagcccatt tcggtcagac gcattgcacc
    58801 catgtaggaa ccgtcaccgc cgataaccac cagcgcgtcg ataccacgtt ttttcaggtt
    58861 ttcgatagcc acggcgcgga tgttctcgtc gcggaattcc gggaaacgcg cagaaccgag
    58921 gaacgtaccg ccacggttga tcatgtcaga cacgctgtaa cggtctagct gtaccatacg
    58981 gtcttcatac agacccagat agccgtcata aatgcccatt acttccagac cttctgtcag
    59041 cgcagaacga acaaccccgc gaattgcggc gttcatgcct ggcgcatcac cgccgcttgt
    59101 caacacaccg attttcttaa tcatgactac ctctgaactt tggaatgcaa aataaaatct
    59161 gttgccggaa gtcttcttgc acatcgaagt gatccaacga atgtgcaaat agtataacaa
    59221 tcacttcctg ctgaattgat tcaggtcagg ccaaatggcg gtattttata cacaaaatgc
    59281 gggtctggct ctcttttata ctgattatga aagcatagac cgtttaccct ccctgggtac
    59341 gacggaacag gggtcctgat ggataattac atccgatccc ggaaaacgcc gtaaaatagc
    59401 ctgctctacc tgatccgcca ccatatgtgc ctgaaccaaa ggcagagagt cttccatttc
    59461 caaatgaatc tgaataaagc gggtcggccc tgactgccgc gtgcgaagat cgtgagcgcc
    59521 gctaacaccc ggccaggaag tcacgatatc aataatttct tgccgttcct catcaggcaa
    59581 tgcgcgatcc agtaatgact gtaccgcctc atatcccatg cgtaacgcgc tatataaaat
    59641 atagatgccg attcccaatg caaacagagc atcggcgcga tgccagccgt accaggacaa
    59701 ccccagcgcc agcagaattg cgccgttcat cataacatca gactggtaat gtagcatatc
    59761 agcccgcacc gcctggcttt gcgtccgccg caccacccaa cgctgaaacg agacaaggat
    59821 aatcgtacaa attagcgcca caattgtcac gataaccccg acgcctggat ctgtcatcgg
    59881 tgttggagat atcagatgtt gaatacccgt caaaaacagg aatagtgccg aaccggagat
    59941 aaacatactt tgcgccagcg ccgcgaggga ctctgcttta ccgtgaccaa acgagtgatt
    60001 atcgtcggca ggttgcaggg aatatcgcac caccagtaaa ttcgtcaacg acgcgccgat
    60061 atccaccagc gaatccacca gcgcggcgag aatactcacc gacccggtat accaccatgc
    60121 aaaaatttta atcagcaata gcaacgaagc catcgccgtc gcagcaatcg ccgcccgact
    60181 gaccagccgt ccataagatt gattcataaa tactcccgct atcaactgac gctagtataa
    60241 cggaagcaaa tcatctgcaa tgcattaagc agcaggcaaa ttgaggataa aaaaaacccc
    60301 cacatcatgt gggggaagac agggatggtg tctatggcaa ggaaaacagg gtttactact
    60361 gggaacgtga gttgctacta ctcaatagct tcaacgatga acttttttgc cattgcgtca
    60421 cgtcgcgcaa ctgctccatt cgttgttgat gtttctcgtt taaaaccgct tgctgctccg
    60481 gcgttaacag gcgatacatt tggttgcgga cttttgccat ctcaacctga cgagcaattt
    60541 gctcattcgc cattttttct gcctgtgcgc gcacagcgtt ttcatcaaaa ttttctgcgg
    60601 tgacaaggcg atgcattgtc tccagttcgc taacattaac aggaggctgt tcgtgccggg
    60661 cctgttgcat aagatctcgc atctgctgac gctgatgttc ggttaaactt atgccgtcga
    60721 acatatggct ctgcgtactg cgctgcgtaa gttcttcacc cggatgccag ttatcgcctg
    60781 aaccgacttc agcagcgtgg cttaatgaac tgactgccag cgttgaggcc atgacggcag
    60841 cggtaactat gcgcatcatt tgctcccaaa atctttctgt cgcgattcaa cgatagagag
    60901 tttacgattc aggctgcaaa catgcgtcag ggggtgtaaa acaacgtaaa gtcatggatt
    60961 agcgacgtct gatgacgtaa tttctgcctc ggaggtattt aaacaatgaa taaaatcctg
    61021 ttagttgatg atgaccgaga gctgacttcc ctattaaagg agctgctcga gatggaaggc
    61081 ttcaacgtga ttgttgccca cgatggggaa caggcgcttg atcttctgga cgacagcatt
    61141 gatttacttt tgcttgacgt aatgatgccg aagaaaaatg gtatcgacac attaaaagca
    61201 cttcgccaga cacaccagac gcctgtcatt atgttgacgg cgcgcggcag tgaacttgat
    61261 cgcgttctcg gccttgagct gggcgcagat gactatctcc cgaaaccgtt taatgatcgt
    61321 gagctggtgg cacgtattcg cgcgatcctg cgccgttcgc actggagcga gcaacagcaa
    61381 aacaacgaca acggttcacc gacactggaa gttgatgcct tagtgctgaa tccaggccgt
    61441 caggaagcca gcttcgacgg gcaaacgctg gagttaaccg gtactgagtt taccctgctc
    61501 tatttgctgg cacagcatct gggtcaggtg gtttcccgtg aacatttaag ccaggaagtg
    61561 ctgggcaaac gcctgacgcc tttcgaccgc gctatcgata tgcacatttc caacctgcgt
    61621 cgtaaactgc cggatcgtaa agatggtcac ccgtggttta aaaccttgcg tggtcgcggc
    61681 tacctgatgg tttctgcttc atgataggca gcttaaccgc gcgcatcttc gccatcttct
    61741 ggctgacgct ggcgctggtg ttgatgttgg ttttgatgtt acccaagctc gattcacgcc
    61801 agatgaccga gcttctggat agcgaacagc gtcaggggct gatgattgag cagcatgttg
    61861 aagcggagct ggcgaacgat ccgcccaacg atttaatgtg gtggcggcgt ctgttccggg
    61921 cgattgataa gtgggcaccg ccaggacagc gtttgttatt ggtgaccacc gaaggccgcg
    61981 tgatcggcgc tgaacgcagc gaaatgcaga tcattcgtaa ctttattggt caggccgata
    62041 acgccgatca tccgcagaag aaaaagtatg gccgcgtgga actggtcggt ccgttctccg
    62101 tacgtgatgg cgaagataat taccaacttt atctgattcg tccggccagc agttctcaat
    62161 ccgatttcat taacttactg tttgaccgcc cgctattact gctgattgtc accatgttgg
    62221 tcagtacgcc gctgctgttg tggttggcct ggagtctggc aaaaccggcg cgtaagctga
    62281 aaaacgctgc cgatgaagtt gcccagggaa acttacgcca gcacccggaa ctggaagcgg
    62341 ggccacagga attccttgcc gcaggtgcca gttttaacca gatggtcacc gcgctggagc
    62401 gcatgatgac ctctcagcag cgtctgcttt ctgatatctc tcacgagctg cgcaccccgc
    62461 tgacgcgtct gcaactgggt acggcgttac tgcgccgtcg tagcggtgaa agcaaggaac
    62521 tggagcgtat tgaaaccgaa gcgcaacgtc tggacagcat gatcaacgat ctgttggtga
    62581 tgtcccgtaa tcagcaaaaa aacgcgctgg ttagcgaaac catcaaagcc aaccagttgt
    62641 ggagtgaagt gctggataac gcggcgttcg aagccgagca gatgggcaag tcgttgacgg
    62701 ttaacttccc gcctgggccg tggccgctgt acggcaatcc gaacgccctg gaaagtgcgc
    62761 tggaaaacat tgttcgtaat gctctgcgtt attcccatac gaagattgaa gtgggctttg
    62821 cggtagataa agacggtatc accattacgg tggacgacga tggtcctggc gttagcccgg
    62881 aagatcgcga acagattttt cgtccgttct atcggaccga tgaagcacgc gatcgtgaat
    62941 ctggcggtac aggattgggg ctggcgattg ttgaaaccgc cattcagcag catcgtggct
    63001 gggtgaaggc agaagacagc ccgctgggcg gtttacggct ggtgatttgg ttgccgctgt
    63061 ataagcggag ttaaactccg catttgtagg caggataagg cgtttacgcc gcatccggca
    63121 tttgagcagg atgcctgatg cgacgctgat agcgtcttat caggcctaca ctccgggggt
    63181 tattttcccc acaaccggcg ggaaaaatct tcgatcttgc cgctcagtcg gcgcttttgc
    63241 atcgtccgcg tccagctttt cgataacccg gcggcggaga gtaaacggtg atattgatcg
    63301 tcatcaaacg gcatatgcca tgcgatggcg gcagcctcct gcacggtcac atcgctgaca
    63361 cgggaaacca actccagcgg cgcgtccgca gacactttcc ctggcgctat cacgctatac
    63421 aaccacccca ctttacccgt gttttgcatc aactgcgcta tatcgctgat atcaaaatgg
    63481 taattgagtt tatagcaagg cgagcgcggc tggctgacct gaattaatgc ctctccccag
    63541 cggaaaatat cgcccatata aacattactt tccgtgagac cgtcggttga gagattttcg
    63601 ccaaacgcag gcgcaacaaa caactccgcc tgttccggaa attcccgcgc ccagtagaga
    63661 tagtgctcac gaggataatg acacagcgct ctgtctggcc cgccgtgaac ctttttctcc
    63721 gcctgctcgt caccttccag ccccagctcc gtcagcatca actcaccatc aacctggatt
    63781 ttggcaattg cgctgggttt gccttcggga taagcctgaa tcttgcctgt gtatacatca
    63841 accggatatc gcatctgttc cctctccacg agaaatttcc ccattagacc atatttattt
    63901 tatgtcaggc gtgctgtttt cgcggtaagt atccaggcag gaactcaata gcaaaccgga
    63961 cgaacgttag ttcatccggc tgtggggatt aaggaagcag gtttatttca ccgtacctaa
    64021 aatttcgatt ttcgctgctc tggctttgac tttacgtgac cagatagagg taagaattgg
    64081 caccagaatc gaagtcacaa tgaccgccgt cgctaccagc gaagttgctg ccggagccat
    64141 cggtttaaac gcaggcacca tttctgcaat cagcacaggt gtcgctaccg cggcccctgc
    64201 ggaactggaa gcggcaattc cggcagtgcc atcgccaccg ccaatcaatt tatcggcgat
    64261 aatcaacgga ataccggtca cgataattac tgccacaccc aacaggatcc ccagcaaacc
    64321 agtctgggca atcacagtca aatcaatggt attgcccagc gcgaaggcaa agaatggaat
    64381 cagcgtttgc accgctttgc tgaaaaattc tcgtaattca gggtcaaggt tccccagggc
    64441 aaagcccacc aggaacggta atactgcgcc gacgaaaaca tgcggttcaa acgaggcaat
    64501 ccctgcagtg cccagaataa tcatcgtcat gagcggcccg gactccaacg acatcaacac
    64561 aaatgcccca gcttcttctt ttgtgccgta ctgctgcatg atggaagcgt aaagtccgcc
    64621 attggtcata tccatcgccg ccaccagcgc cagcgttgaa agtccggcaa agaatccaac
    64681 ttcaacacca tgttccggaa taatgcgcga ggcaattgcc gcaaccaccc acgcgacggc
    64741 aatttttgtt accaccaacg taccggattt acgcaatacc gttcccgtcg cgcttaattt
    64801 tattgacgcc cccatgcaaa aaaaccacac cgccagaatg ggcaccgtac cggtaatcat
    64861 cccgttggtg aatgatccaa aatatttccc cgcccccggc gagaaggtgt ggcacagtgc
    64921 gccaaggaat agcgggacga gcatcatccc ccccgggatt ttctcaatcg agcgttttat
    64981 ctgcatttcc atcacctgtc ggcatatttt taagggctga tgcgtcattc ctcaatggag
    65041 catcagtgtt gatggaaacg aagatagaca ttcatttaat caataaaagt gatcaaaacc
    65101 acatcaattg aaacgctgtt ttatttttat cggatcattg tttttatttt gaaattgatc
    65161 acaaaaaaac caccgccgtt ggcgatggtt cattttttaa gctgatatgc ggcctatacg
    65221 cctcattgca gcaggcggca aatgattatt ttttcgccgc aaaacgtgcc gctgcttcgt
    65281 cccagttcac cacgttccag aactctttaa tgtagtccgg acggcggttc tggaatttca
    65341 ggtagtaagc atgttcccac acatccaggc ccataatcgg gaagccggaa gcgccagaaa
    65401 tagcttcacc catcagcgga gaatcctggt tagcagtaga aaccaccgcc agtttatcgc
    65461 ctttcagcac cagccatgcc cagccggaac caaagcggga agctgccgct ttttcaaatt
    65521 ctgctttgaa gttatcaacg gagccgaagt cacgttcgat agccgctttc aggtcaccct
    65581 gcagggtggt gccttttttc agacctttcc agaacaggct gtggttagcg tgaccgccag
    65641 cgttgttgcg cagtacggtt ttcttgtctg ctggcagctg gtccagtttg gtgatcagct
    65701 cttcaaccgg caggttggca aattctggca ggctttccag cgccgcgttg gcgttgttta
    65761 cgtaggtctg atggtgtttg gtgtggtgga tttccatggt ctgcttatcg aagtgcggtt
    65821 ccagggcatc gtaagcatac ggcagggatg gcagggtata gctcatattc atctccagta
    65881 ttgtcgggcg gccgattgtt aatgccgcgt aagcagttgg ttcattatag ttaattaaat
    65941 gatattgaaa atgattatca atgccgtact tttcgtaagg gtatggtttt gcaggaaaat
    66001 gcccgagatg tgaagcaaat cacccactta atgccgtgat tgccagtaaa tcgacaacgg
    66061 cggcaacagg cgaaaggtta atcgacagca cgatttttac actcatctcg tcggagatgt
    66121 gacgcgacga aaaatgatga ggataagaag atgagtaacg cgattacgat ggggatattt
    66181 tggcatttga tcggcgcggc cagtgcagcc tgtttttacg ctccgttcaa aaaagtaaaa
    66241 aaatggtcat gggaaaccat gtggtcagtc ggtgggattg tttcgtggat tattctgccg
    66301 tgggccatca gcgccctgtt actaccgaat ttctgggcgt attacagctc gtttagtctc
    66361 tctacgctac tgcctgtttt tctgttcggc gctatgtggg ggatcggtaa tatcaactac
    66421 ggcctgacca tgcgttatct cggcatgtcg atgggaattg gcatcgccat tggcattacg
    66481 ttgattgtcg gtacgctgat gacgccaatt atcaacggca atttcgatgt gttgattagc
    66541 accgaaggcg gacgcatgac gttgctcggc gttctggtgg cgctgattgg cgtagggatt
    66601 gtaactcgcg ccgggcagtt gaaagagcgc aagatgggca ttaaagccga agagttcaat
    66661 ctgaaaaaag ggctggtgct ggcggtgatg tgcggcattt tctctgccgg gatgtccttt
    66721 gcgatgaacg ccgcaaaacc gatgcatgaa gccgctgccg cacttggcgt cgatccactg
    66781 tatgtcgctc tgccaagcta tgttgtcatc atgggcggcg gcgcgatcat taacctcggt
    66841 ttctgtttta ttcgtctggc aaaagtgaag gatttgtcgc taaaagccga cttctcgctg
    66901 gcaaaatcgc tgatcattca caatgtgtta ctctcgacac tgggcgggtt gatgtggtat
    66961 ctgcaattct ttttctatgc ctggggccac gcccgcattc cggcgcagta tgactacatc
    67021 agttggatgc tgcatatgag tttctatgta ttgtgcggcg gtatcgtcgg gctggtgctg
    67081 aaagagtgga acaatgcagg acgccgtccg gtaacggtgt tgagcctcgg ttgtgtggtg
    67141 attattgtcg ccgctaacat cgtcggcatc ggcatggcga attaatcttt ctgcgaattg
    67201 agatgacgcc actggctggg cgtcatcccg gtttcccggg taaacaccac cgaaaaatag
    67261 ttactatctt caaagccaca ttcggtcgaa atatcactga ttaacaggcg gctatgctgg
    67321 agaagatatt gcgcatgaca cactctgacc tgtcgcagat attgattgat ggtcattcca
    67381 gtctgctggc gaaattgctg acgcaaaacg cgctcactgc acgatgcctc atcacaaaat
    67441 ttatccagcg caaagggact tttcaggcta gccgccagcc gggtaatcag cttatccagc
    67501 aacgtttcgc tggatgttgg cggcaacgaa tcactggtgt aacgatggcg attcagcaac
    67561 atcaccaact gcccgaacag caactcagcc atttcgttag caaacggcac atgctgacta
    67621 ctttcatgct caagctgacc gataacctgc cgcgcctgcg ccatccccat gctacctaag
    67681 cgccagtgtg gttgccctgc gctggcgtta aatcccggaa tcgccccctg ccagtcaaga
    67741 ttcagcttca gacgctccgg gcaataaata atattctgca aaaccagatc gttaacggaa
    67801 gcgtaggagt gtttatcgtc agcatgaatg taaaagagat cgccacgggt aatgcgataa
    67861 gggcgatcgt tgagtacatg caggccatta ccgcgccaga caatcaccag ctcacaaaaa
    67921 tcatgtgtat gttcagcaaa gacatcttgc ggataacggt cagccacagc gactgcctgc
    67981 tggtcgctgg caaaaaaatc atctttgaga agttttaact gatgcgccac cgtggctacc
    68041 tcggccagag aacgaagttg attattcgca atatggcgta caaatacgtt gagaagattc
    68101 gcgttattgc agaaagccat cccgtccctg gcgaatatca cgcggtgacc agttaaactc
    68161 tcggcgaaaa agcgtcgaaa agtggttact gtcgctgaat ccacagcgat aggcgatgtc
    68221 agtaacgctg gcctcgctgt ggcgtagcag atgtcgggct ttcatcagtc gcaggcggtt
    68281 caggtatcgc tgaggcgtca gtcccgtttg ctgcttaagc tgccgatgta gcgtacgcag
    68341 tgaaagagaa aattgatccg ccacggcatc ccaattcacc tcatcggcaa aatggtcctc
    68401 cagccaggcc agaagcaagt tgagacgtga tgcgctgttt tccaggttct cctgcaaact
    68461 gcttttacgc agcaagagca gtaattgcat aaacaagatc tcgcgactgg cggtcgaggg
    68521 taaatcattt tccccttcct gctgttccat ctgtgcaacc agctgtcgca cctgctgcaa
    68581 tacgctgtgg ttaacgcgcc agtgagacgg atactgccca tccagctctt gtggcagcaa
    68641 ctgattcagc ccggcgagaa actgaaatcg atccggcgag cgatacagca cattggtcag
    68701 acacagatta tcggtatgtt catacagatg ccgatcatga tcgcgtacga aacagaccgt
    68761 gccaccggtg atggtatagg gctgcccatt aaacacatga atacccgtgc catgttcgac
    68821 aatcacaatt tcatgaaaat catgatgatg ttcaggaaaa tccgcctgcg ggagccgggg
    68881 ttctatcgcc acggacgcgt taccagacgg aaaaaaatcc acactatgta atacggtcat
    68941 actggcctcc tgatgtcgtc aacacggcga aatagtaatc acgaggtcag gttcttacct
    69001 taaattttcg acggaaaacc acgtaaaaaa cgtcgatttt tcaagataca gcgtgaattt
    69061 tcaggaaatg cggtgagcat cacatcacca caattcagca aattgtgaac atcatcacgt
    69121 tcatctttcc ctggttgcca atggcccatt ttcctgtcag taacgagaag gtcgcgaatt
    69181 caggcgcttt ttagactggt cgtaatgaaa ttcagcagga tcacattatg acctttcgca
    69241 attgtgtcgc cgtcgatctc ggcgcatcca gtgggcgcgt gatgctggcg cgttacgagc
    69301 gtgaatgccg cagcctgacg ctgcgcgaaa tccatcgttt taacaatggg ctgcatagtc
    69361 agaacggcta tgtcacctgg gatgtggata gccttgaaag tgccattcgc cttggattaa
    69421 acaaggtgtg cgaggaaggg attcgtatcg atagcattgg gattgatacc tggggcgtgg
    69481 actttgtgct gctcgaccaa cagggtcagc gtgtgggcct gcccgttgct tatcgcgata
    69541 gccgcaccaa tggcctaatg gcgcaggcac aacaacaact cggcaaacgc gatatttatc
    69601 aacgtagcgg catccagttt ctgcccttca atacgcttta tcagttgcgt gcgctgacgg
    69661 agcaacaacc tgaacttatt ccacacattg ctcacgctct gctgatgccg gattacttca
    69721 gttatcgcct gaccggcaag atgaactggg aatataccaa cgccacgacc acgcaactgg
    69781 tcaatatcaa tagcgacgac tgggacgagt cgctactggc gtggagcggg gccaacaaag
    69841 cctggtttgg tcgcccgacg catccgggta atgtcatagg tcactggatt tgcccgcagg
    69901 gtaatgagat tccagtggtc gccgttgcca gccatgatac cgccagcgcg gttatcgcct
    69961 cgccgttaaa cggctcacgt gctgcttatc tctcttctgg cacctggtca ttgatgggct
    70021 tcgaaagcca gacgccattt accaatgaca cggcactggc agccaacatc accaatgaag
    70081 gcggggcgga aggtcgctat cgggtgctga aaaatattat gggcttatgg ctgcttcagc
    70141 gagtgcttca ggagcagcaa atcaacgatc ttccggcgct tatctccgcg acacaggcac
    70201 ttccggcttg ccgcttcatt atcaatccca atgacgatcg ctttattaat cctgagacga
    70261 tgtgcagcga aattcaggct gcgtgtcggg aaacggcgca accgatcccg gaaagtgatg
    70321 ctgaactggc gcgctgcatt ttcgacagtc tggcgctgct gtatgccgat gtgttgcatg
    70381 agctggcgca gctgcgcggt gaagatttct cgcaactgca tattgtcggc ggaggctgcc
    70441 agaacacgct gctcaaccag ctatgcgccg atgcctgcgg tattcgggtg atcgccgggc
    70501 ctgttgaagc ctcgacgctc ggcaatatcg gcatccagtt aatgacgctg gatgaactca
    70561 acaatgtgga tgatttccgt caggtcgtca gcaccaccgc gaatctgacc acctttaccc
    70621 ctaatcctga cagtgaaatt gcccactatg tggcgcagat tcactctaca cgacagacaa
    70681 aggagctttg cgcatgacca ctcaactgga acaggcctgg gagctagcga aacagcgttt
    70741 cgcggcggtg gggattgatg tcgaggaggc gctgcgccaa cttgatcgtt tacccgtttc
    70801 aatgcactgc tggcagggcg atgatgtttc cggttttgaa aacccggaag gttcgctgac
    70861 cggggggatt caggccacag gcaattatcc gggcaaagcg cgtaatgcca gtgagctacg
    70921 tgccgatctg gaacaggcta tgcggctgat tccggggccg aaacggctta atttacatgc
    70981 catctatctg gaatcagata cgccagtctc gcgcgaccag atcaaaccag agcacttcaa
    71041 aaactgggtt gaatgggcga aagccaatca gctcggtctg gattttaacc cctcctgctt
    71101 ttcgcatccg ctaagcgccg atggctttac gctttcccat gccgacgaca gcattcgcca
    71161 gttctggatt gatcactgca aagccagccg tcgcgtttcg gcctattttg gcgagcaact
    71221 cggcacacca tcggtgatga acatctggat cccggatggt atgaaagata tcaccgttga
    71281 ccgtctcgcc ccgcgtcagc gtctgctggc agcactggat gaggtgatca gcgagaagct
    71341 aaaccctgcg caccatatcg acgccgttga gagcaaattg tttggcattg gcgcagagag
    71401 ctacacggct ggctccaatg agttttacat ggggtatgcc accagccgcc agactgcgct
    71461 gtgcctggac gccgggcact tccacccgac tgaagtgatt tccgacaaga tttccgccgc
    71521 catgctgtat gtgccgcagt tgctgctgca cgtcagccgt ccggttcgct gggacagcga
    71581 tcacgtagtg ctgctggatg atgaaaccca ggcaattgcc agtgagattg tgcgtcacga
    71641 tctgtttgac cgggtgcata tcggccttga cttcttcgat gcctctatca accgcattgc
    71701 cgcgtgggtc attggtacac gcaatatgaa aaaagccctg ctgcgtgcgt tgctggaacc
    71761 taccgctgag ctgcgcaagc tggaagcggc gggcgattac actgcgcgtc tggcactgct
    71821 ggaagagcag aaatcgttgc cgtggcaggc ggtctgggaa atgtattgcc aacgtcacga
    71881 tacgccagca ggtagcgaat ggctggagag cgtgcgggct tatgagaaag aaattttgag
    71941 tcgccgcggg taaacactgc cggatgcggc gcgagcgcct tatccggcct acgggtcggc
    72001 aacagttgta ggcctgataa gacgcgacag cgtcgcatca ggcattgatt gccggatgcg
    72061 gcacaagtgc cttatccggc ctacaggtcg gcaatagttg taggcctgat aagacgcgac
    72121 agcgtcgcat caggcattga ttgccggatg cggcacaagt gccttatccg gcctacaggt
    72181 cggcaacagt tgtaggcctg ataagacgcg acagcgtcgc atcaggcatt gattgccgga
    72241 tgcggcacaa gtgccttatc aggcctacag gtcggcaata gttgtaggcc tgataagacg
    72301 cgacagcgtc gcatcaggca ttgattgccg gatgcggcgc gagcgcctta tccggcctac
    72361 gggtcgtgca tccgacaaca ccgaatttac aggaacacag aacatgcaaa acattactca
    72421 gtcctggttt gtccagggaa tgatcaaagc caccaccgac gcctggctga aaggctggga
    72481 tgagcgcaac ggcggcaacc tgacgctacg cctggatgac gccgatatcg caccatatca
    72541 cgacaatttc caccaacaac cgcgctatat cccgctcagc cagcccatgc ctttactggc
    72601 aaatacaccg tttattgtca ccggctcggg caaattcttc cgtaacgtcc agcttgatcc
    72661 tgcggctaac ttaggcatcg taaaagtcga cagcgacggc gcgggctacc acattctttg
    72721 ggggttaacc aacgaagccg tccccacttc cgaacttccg gctcacttcc tttcccactg
    72781 cgagcgcatt aaagccacca acggcaaaga tcgggtgatc atgcactgcc acgccaccaa
    72841 cctgatcgcc ctcacctatg tacttgaaaa cgacaccgcg gtcttcactc gccaactgtg
    72901 ggaaggcagc accgagtgtc tggtggtatt cccggatggc gttggcattt tgccgtggat
    72961 ggtgcccggc acggacgaaa tcggccaggc gaccgcacaa gagatgcaaa aacattcgct
    73021 ggtgttgtgg cccttccacg gcgtcttcgg cagcggaccg acgctggatg aaaccttcgg
    73081 tttaatcgac accgcagaaa aatcagcaca agtattagtg aaggtttatt cgatgggcgg
    73141 catgaaacag accatcagcc gtgaagagtt gatagcgctc ggcaagcgtt tcggcgttac
    73201 gccactcgcc agtgcgctgg cgctgtaagg agcaaacatg atccgcaaag cctttgtcat
    73261 gcaggtaaac cccgacgccc acgaagagta tcagcgtcgg cataatccca tctggccaga
    73321 actggaagca gtgctgaaat ctcacggtgc gcataactac gccatctatc tcgacaaagc
    73381 gcgtaatctg ctgtttgcca tggtagagat tgaatctgaa gaacgctgga atgcggttgc
    73441 cagcactgat gtttgccaac gttggtggaa atatatgacc gatgttatgc ccgctaaccc
    73501 ggataacagc ccggtgagta gcgagctgca agaagtgttt tacctgccgt aattgcccaa
    73561 tgtgccagag atgcctccgg cacaatattt t
//
LOCUS       DAPHBJ010000004        73344 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.004, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000004 DAPHBJ010000000
VERSION     DAPHBJ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 73344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.004"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            77..520
                     /gene="cmtB"
                     /locus_tag="RHZ76_000220"
     CDS             77..520
                     /gene="cmtB"
                     /locus_tag="RHZ76_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HDV2810925.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQETIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            548..1936
                     /gene="cmtA"
                     /locus_tag="RHZ76_000221"
     CDS             548..1936
                     /gene="cmtA"
                     /locus_tag="RHZ76_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDV2810926.1"
                     /translation="MENKSARAKVQAFEGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1951..3228
                     /locus_tag="RHZ76_000222"
     CDS             1951..3228
                     /locus_tag="RHZ76_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDV2810927.1"
                     /translation="MKTKVAAIYGKRDVRLRVFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDAGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3225..4190
                     /gene="yggF"
                     /locus_tag="RHZ76_000223"
     CDS             3225..4190
                     /gene="yggF"
                     /locus_tag="RHZ76_000223"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDV2810928.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAASAIDLSLPLTDNLRNVAKALGKPLDKLRIVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4212..4721
                     /gene="fumE"
                     /locus_tag="RHZ76_000224"
     CDS             4212..4721
                     /gene="fumE"
                     /locus_tag="RHZ76_000224"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HDV2810929.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHTILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4718..5431
                     /locus_tag="RHZ76_000225"
     CDS             4718..5431
                     /locus_tag="RHZ76_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HDV2810930.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLHMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLTENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WLELASFCDFSIFIHAPAQILRERLISRKIAGGLTRQVAEAFYARTDGPNVERVLMNS
                     RQANLIVEMTEEGRYHFTS"
     gene            5716..6735
                     /gene="epd"
                     /locus_tag="RHZ76_000226"
     CDS             5716..6735
                     /gene="epd"
                     /locus_tag="RHZ76_000226"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HDV2810931.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNNLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            6785..7948
                     /gene="pgk"
                     /locus_tag="RHZ76_000227"
     CDS             6785..7948
                     /gene="pgk"
                     /locus_tag="RHZ76_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDV2810932.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            8163..9242
                     /gene="fbaA"
                     /locus_tag="RHZ76_000228"
     CDS             8163..9242
                     /gene="fbaA"
                     /locus_tag="RHZ76_000228"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HDV2810933.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            9600..10460
                     /gene="mscS"
                     /locus_tag="RHZ76_000229"
     CDS             9600..10460
                     /gene="mscS"
                     /locus_tag="RHZ76_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HDV2810934.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            10599..11234
                     /gene="argO"
                     /locus_tag="RHZ76_000230"
     CDS             10599..11234
                     /gene="argO"
                     /locus_tag="RHZ76_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HDV2810935.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VMKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLALLAAWLAPRLRTAKAQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            11327..12067
                     /locus_tag="RHZ76_000231"
     CDS             11327..12067
                     /locus_tag="RHZ76_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HDV2810936.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            12234..13130
                     /locus_tag="RHZ76_000232"
     CDS             12234..13130
                     /locus_tag="RHZ76_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2810937.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSITALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(13127..14605)
                     /gene="scpC"
                     /locus_tag="RHZ76_000233"
     CDS             complement(13127..14605)
                     /gene="scpC"
                     /locus_tag="RHZ76_000233"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HDV2810938.1"
                     /translation="METQWTRMTANEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     VIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     LMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(14629..15414)
                     /gene="scpB"
                     /locus_tag="RHZ76_000234"
     CDS             complement(14629..15414)
                     /gene="scpB"
                     /locus_tag="RHZ76_000234"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HDV2810939.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(15425..16420)
                     /gene="meaB"
                     /locus_tag="RHZ76_000235"
     CDS             complement(15425..16420)
                     /gene="meaB"
                     /locus_tag="RHZ76_000235"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HDV2810940.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(16413..18557)
                     /gene="scpA"
                     /locus_tag="RHZ76_000236"
     CDS             complement(16413..18557)
                     /gene="scpA"
                     /locus_tag="RHZ76_000236"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HDV2810941.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVHQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(18761..19654)
                     /gene="argP"
                     /locus_tag="RHZ76_000237"
     CDS             complement(18761..19654)
                     /gene="argP"
                     /locus_tag="RHZ76_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HDV2810942.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            19796..20026
                     /locus_tag="RHZ76_000238"
     CDS             19796..20026
                     /locus_tag="RHZ76_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2810943.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLVNGELVRLFAPWSGIP
                     RLLHALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            20082..20741
                     /gene="rpiA"
                     /locus_tag="RHZ76_000239"
     CDS             20082..20741
                     /gene="rpiA"
                     /locus_tag="RHZ76_000239"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDV2810944.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            20997..22229
                     /gene="serA"
                     /locus_tag="RHZ76_000240"
     CDS             20997..22229
                     /gene="serA"
                     /locus_tag="RHZ76_000240"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDV2810945.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            22458..22517
                     /gene="ibsC"
                     /locus_tag="RHZ76_000241"
     CDS             22458..22517
                     /gene="ibsC"
                     /locus_tag="RHZ76_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HDV2810946.1"
                     /translation="MMRLVIILIVLLLISFSAY"
     gene            complement(22618..23220)
                     /locus_tag="RHZ76_000242"
     CDS             complement(22618..23220)
                     /locus_tag="RHZ76_000242"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDV2810947.1"
                     /translation="MTQLPELPLILSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(23241..23424)
                     /gene="ssrS"
                     /locus_tag="RHZ76_000243"
     ncRNA           complement(23241..23424)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="RHZ76_000243"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23466..23795)
                     /gene="zapA"
                     /locus_tag="RHZ76_000244"
     CDS             complement(23466..23795)
                     /gene="zapA"
                     /locus_tag="RHZ76_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDV2810948.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            23963..24541
                     /gene="ygfB"
                     /locus_tag="RHZ76_000245"
     CDS             23963..24541
                     /gene="ygfB"
                     /locus_tag="RHZ76_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HDV2810949.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24567..25892
                     /gene="pepP"
                     /locus_tag="RHZ76_000246"
     CDS             24567..25892
                     /gene="pepP"
                     /locus_tag="RHZ76_000246"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HDV2810950.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            25889..27067
                     /gene="ubiH"
                     /locus_tag="RHZ76_000247"
                     /gene_synonym="visB"
     CDS             25889..27067
                     /gene="ubiH"
                     /locus_tag="RHZ76_000247"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HDV2810951.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            27090..28292
                     /gene="ubiI"
                     /locus_tag="RHZ76_000248"
                     /gene_synonym="visC"
     CDS             27090..28292
                     /gene="ubiI"
                     /locus_tag="RHZ76_000248"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HDV2810952.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(28366..28449)
                     /locus_tag="RHZ76_000249"
     CDS             complement(28366..28449)
                     /locus_tag="RHZ76_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2810953.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            28739..29833
                     /gene="gcvT"
                     /locus_tag="RHZ76_000250"
     CDS             28739..29833
                     /gene="gcvT"
                     /locus_tag="RHZ76_000250"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HDV2810954.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            29856..30245
                     /gene="gcvH"
                     /locus_tag="RHZ76_000251"
     CDS             29856..30245
                     /gene="gcvH"
                     /locus_tag="RHZ76_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDV2810955.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATTYEALLEDE"
     gene            30363..33236
                     /gene="gcvP"
                     /locus_tag="RHZ76_000252"
     CDS             30363..33236
                     /gene="gcvP"
                     /locus_tag="RHZ76_000252"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HDV2810956.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFNVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            33502..34245
                     /locus_tag="RHZ76_000253"
     CDS             33502..34245
                     /locus_tag="RHZ76_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV2810957.1"
                     /translation="MAIALVTGGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(34302..35735)
                     /gene="bglA"
                     /locus_tag="RHZ76_000254"
     CDS             complement(34302..35735)
                     /gene="bglA"
                     /locus_tag="RHZ76_000254"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HDV2810958.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            35780..36091
                     /locus_tag="RHZ76_000255"
     CDS             35780..36091
                     /locus_tag="RHZ76_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDV2810959.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            36255..36914
                     /locus_tag="RHZ76_000256"
     CDS             36255..36914
                     /locus_tag="RHZ76_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDV2810960.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(36991..37971)
                     /gene="ygfZ"
                     /locus_tag="RHZ76_000257"
     CDS             complement(36991..37971)
                     /gene="ygfZ"
                     /locus_tag="RHZ76_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HDV2810961.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            38214..38480
                     /gene="sdhE"
                     /locus_tag="RHZ76_000258"
     CDS             38214..38480
                     /gene="sdhE"
                     /locus_tag="RHZ76_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HDV2810962.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            38461..38868
                     /locus_tag="RHZ76_000259"
     CDS             38461..38868
                     /locus_tag="RHZ76_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HDV2810963.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(38908..39429)
                     /gene="fldB"
                     /locus_tag="RHZ76_000260"
     CDS             complement(38908..39429)
                     /gene="fldB"
                     /locus_tag="RHZ76_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HDV2810964.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            39541..40437
                     /gene="xerD"
                     /locus_tag="RHZ76_000261"
     CDS             39541..40437
                     /gene="xerD"
                     /locus_tag="RHZ76_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDV2810965.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRT"
     gene            40462..41172
                     /gene="dsbC"
                     /locus_tag="RHZ76_000262"
     CDS             40462..41172
                     /gene="dsbC"
                     /locus_tag="RHZ76_000262"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HDV2810966.1"
                     /translation="MKKGFMLFTLLAAFSGFAQDDDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYVLGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            41178..42911
                     /gene="recJ"
                     /locus_tag="RHZ76_000263"
     CDS             41178..42911
                     /gene="recJ"
                     /locus_tag="RHZ76_000263"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDV2810967.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMILKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            43002..44100
                     /gene="prfB"
                     /locus_tag="RHZ76_000264"
     CDS             join(43002..43076,43078..44100)
                     /gene="prfB"
                     /locus_tag="RHZ76_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDV2810968.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNELERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            44110..45627
                     /gene="lysS"
                     /locus_tag="RHZ76_000265"
     CDS             44110..45627
                     /gene="lysS"
                     /locus_tag="RHZ76_000265"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDV2810969.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(45670..46218)
                     /gene="idi"
                     /locus_tag="RHZ76_000266"
     CDS             complement(45670..46218)
                     /gene="idi"
                     /locus_tag="RHZ76_000266"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDV2810970.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            46273..46344
                     /locus_tag="RHZ76_000267"
     CDS             46273..46344
                     /locus_tag="RHZ76_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2810971.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(46341..46466)
                     /locus_tag="RHZ76_000268"
     CDS             complement(46341..46466)
                     /locus_tag="RHZ76_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2810972.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(46468..47916)
                     /gene="uacT"
                     /locus_tag="RHZ76_000269"
     CDS             complement(46468..47916)
                     /gene="uacT"
                     /locus_tag="RHZ76_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HDV2810973.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDLLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            48352..50271
                     /gene="ygfT"
                     /locus_tag="RHZ76_000270"
     CDS             48352..50271
                     /gene="ygfT"
                     /locus_tag="RHZ76_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HDV2810974.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVTIGNLERYITDTALAMGWCPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            50271..50759
                     /locus_tag="RHZ76_000271"
     CDS             50271..50759
                     /locus_tag="RHZ76_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDV2810975.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQANSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(50795..52162)
                     /gene="ghxQ"
                     /locus_tag="RHZ76_000272"
                     /gene_synonym="ygfQ"
     CDS             complement(50795..52162)
                     /gene="ghxQ"
                     /locus_tag="RHZ76_000272"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HDV2810976.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(52198..53514)
                     /gene="guaD"
                     /locus_tag="RHZ76_000273"
     CDS             complement(52198..53514)
                     /gene="guaD"
                     /locus_tag="RHZ76_000273"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HDV2810977.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWENGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(53532..54932)
                     /gene="xanQ"
                     /locus_tag="RHZ76_000274"
     CDS             complement(53532..54932)
                     /gene="xanQ"
                     /locus_tag="RHZ76_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HDV2810978.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(55097..57967)
                     /locus_tag="RHZ76_000275"
     CDS             complement(55097..57967)
                     /locus_tag="RHZ76_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HDV2810979.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKANIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IALTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(57964..58743)
                     /locus_tag="RHZ76_000276"
     CDS             complement(57964..58743)
                     /locus_tag="RHZ76_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HDV2810980.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(58794..60122)
                     /gene="ssnA"
                     /locus_tag="RHZ76_000277"
     CDS             complement(58794..60122)
                     /gene="ssnA"
                     /locus_tag="RHZ76_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDV2810981.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SFKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDITLL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(60125..63223)
                     /gene="ygfK"
                     /locus_tag="RHZ76_000278"
     CDS             complement(60125..63223)
                     /gene="ygfK"
                     /locus_tag="RHZ76_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HDV2810982.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTG
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTIEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLLVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFNLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSKGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(63545..64123)
                     /gene="mocA"
                     /locus_tag="RHZ76_000279"
     CDS             complement(63545..64123)
                     /gene="mocA"
                     /locus_tag="RHZ76_000279"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDV2810983.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAFQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAKKRYTEI"
     gene            64226..64996
                     /gene="yqeC"
                     /locus_tag="RHZ76_000280"
     CDS             64226..64996
                     /gene="yqeC"
                     /locus_tag="RHZ76_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDV2810984.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQT
                     RGRRVLITTTTHMFMPKSHWPVVFCRDPAMLLHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            65044..66669
                     /locus_tag="RHZ76_000281"
     CDS             65044..66669
                     /locus_tag="RHZ76_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDV2810985.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(66890..67822)
                     /gene="arcC"
                     /locus_tag="RHZ76_000282"
     CDS             complement(66890..67822)
                     /gene="arcC"
                     /locus_tag="RHZ76_000282"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDV2810986.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(67870..69255)
                     /gene="hyuA"
                     /locus_tag="RHZ76_000283"
     CDS             complement(67870..69255)
                     /gene="hyuA"
                     /locus_tag="RHZ76_000283"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HDV2810987.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(69308..70519)
                     /locus_tag="RHZ76_000284"
     CDS             complement(69308..70519)
                     /locus_tag="RHZ76_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HDV2810988.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(70577..71773)
                     /gene="dpaL"
                     /locus_tag="RHZ76_000285"
     CDS             complement(70577..71773)
                     /gene="dpaL"
                     /locus_tag="RHZ76_000285"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDV2810989.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNTIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(71831..73018)
                     /gene="ygeW"
                     /locus_tag="RHZ76_000286"
     CDS             complement(71831..73018)
                     /gene="ygeW"
                     /locus_tag="RHZ76_000286"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HDV2810990.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
BASE COUNT        17698 a        18258 c        19466 g        17922 t
ORIGIN      
        1 attgctttca ttaaaccgca cgagtatttt atttttaccg ccggacggta ccctaaaaat
       61 aaaataagga taatttatgc ggcttagtga ttattttcca gaatcatcaa tctcagttat
      121 acattcagca aaagattggc aggaaactat cgatttctcg atggtatcat tgctggataa
      181 aaactatatc agcgagaatt acattcaggc aattaaagat tccaccatta acaatggccc
      241 ttattatatt ctcgcaccag gcgtggcaat gcctcatgcg cgaccggaat gtggggcgct
      301 taaaaccggg atgtctttga cattacttga acaaggtgtt tattttccgg ggaatgacga
      361 accaattaaa ttactcatcg gactctctgc tgccgatgcc gattcgcaca ttggcgctat
      421 tcaggcgtta agtgaattac tgtgcgaaga agaaatactc gaacaactct taacagcatc
      481 atcagaaaaa caattagcgg acattatcag ccgcggataa ttactctctc ctctacagga
      541 taatattatg gaaaacaagt ctgctcgtgc aaaggtccag gcttttgagg gctttttgac
      601 tgcaatggtc atccccaata ttggtgcttt tattgcctgg ggttttatta ctgcgttatt
      661 tattcccacc ggttggctgc ctaacgaaca tttcgccaaa attgtcggcc cgatgattac
      721 ctatttattg cccgtgatga ttggttctac aggtggtcat ctggtcggcg gtaaacgcgg
      781 ggcggtcatg ggcggaatag gtactattgg tgtgatcgtt ggcgcagaga tcccgatgtt
      841 ccttggctca atgatcatgg ggccgctcgg tgggttggtc ataaaatatg tcgataaggc
      901 actggaaaaa cgcatacctg ccggttttga gatggttatc aataacttct cattaggtat
      961 cgcggggatg ctcctttgtc tgctgggttt tgaagttatc ggcccggcgg tgttaattgc
     1021 caatactttc gtcaaagagt gtattgaggc gctggtacat gcgggttatc tgccattgtt
     1081 gtcagtcatc aatgaaccgg cgaaagtgct tttcctcaat aatgcgatcg atcagggcgt
     1141 ctattatccg ctgggaatgc aacaggcttc ggttaacggt aaatccatct tctttatggt
     1201 ggcctctaac ccaggtccgg gcctggggct gctgctggcg tttaccttgt ttggtaaagg
     1261 gatgagtaaa cgttctgcgc ccggggcgat gattattcac ttcctcggtg ggatccacga
     1321 actgtatttc ccgtatgtgc tgatgaagcc gctgaccatt attgccatga ttgcgggcgg
     1381 tatgtctggc acctggatgt ttaacttact ggacggtggt ctggtggctg gcccaagtcc
     1441 ggggtctatc tttgcttacc tggcactgac gccgaaaggc tcgttcctgg cgacaattgc
     1501 cggtgttacg gtaggtaccc tggtgtcctt tgctattact tcgctgatac tgaagatgga
     1561 aaaaacggtg gaaacggaga gcgaagatga gtttgctcag tcagccaatg cggttaaggc
     1621 gatgaaacaa gagggtgcat tctcgttaag cagggttaag cgtatcgcct ttgtttgcga
     1681 tgcggggatg ggctccagtg cgatgggcgc gaccaccttc cgtaaacgcc tggaaaaagc
     1741 ggggctggca attgaagtaa aacattacgc catagaaaac gtgcctgcgg atgcggatat
     1801 cgtcgttact catgccagtc tggaagggcg cgtgaaacgt gtgacggata aaccactgat
     1861 attgattaat aactatattg gcgatccaaa actcgacact ttatttaatc aattaaccgc
     1921 cgaacataaa cactgattac agaggtaaaa atgaaaacca aagttgctgc tatttatggc
     1981 aagcgggatg tccgtctgcg cgtatttgaa ctgccagaaa ttaccgataa tgaattactg
     2041 gtgagtgtaa tttctgacag cgtctgttta tcgacctgga aagcggcgtt actcggtagt
     2101 gaacataaac gcgtacccga cgatttagaa aatcatccgg tcattaccgg gcatgaatgt
     2161 gccggggtta ttgtcgaagt gggtaaaaat ctcactggca aatataaaaa aggccagcgt
     2221 tttgtattgc aaccggcgat ggggttacca agcggatatt cagcgggcta cagctacgaa
     2281 tattttggcg gcaatgccac ttatatgatt attcccgaaa tcgccattaa tttgggctgc
     2341 gtattaccgt atcacggctc ttattttgct gcggcgtcgc tggcagagcc tatgtgctgc
     2401 attattggtg cttatcatgc caattatcac accacgcaat atgtttatga gcatcgcatg
     2461 ggcgtcaaac ctggcggcaa tattgcactg ctggcgtgtg caggtccgat gggcattggc
     2521 gctatcgatt acgccattaa cggcggcata caaccgtcgc gggtggtggt ggtcgatatc
     2581 gacgacaaac gtctggcgca ggtacagaag ctgctgccgg tggaactggc ggccagtaaa
     2641 ggcattgagc tggtgtatgt gaataccaaa gggatgagcg atcctgtcca gatgctgcgg
     2701 gcgctgacag gagatgccgg gttcgatgac atttttgttt atgcggcggt gcctgctgtc
     2761 gttgagatgg ctgatgaatt actggcggaa gatggctgtc tgaacttctt tgccgggccg
     2821 acggataaaa acttcaaagt gccgtttaat ttctacaacg tccattacaa cagcacgcac
     2881 gtcgtcggta catctggcgg ttcaacggac gacatgaaag aggcgattgc ccttagcgcc
     2941 actgggcagt tacagccgtc gtttatggtg acccatatcg gtggcctgga tgcggtgcca
     3001 gaaaccgtgc tcaatctgcc ggatatccct ggcggtaaaa aactcattta taacggcgtg
     3061 accatgccgc tcactgccat tgccgatttt gccgaaaaag gcaaaaccga tccgctgttt
     3121 aaagagttgg cgcggctggt tgaggaaacg cacggcatct ggaatgaaca ggccgagaaa
     3181 tatctgctgg cacaatttgg cgttgatatc ggggaggccg cgcaatgatg tccctggcgt
     3241 ggccattatt tcgcgttacg gaacaggcag cgttggctgc ctggccgcaa accggatgtg
     3301 gcgacaaaaa taaaattgat ggcctggcgg tcaccgcgat gcgccaggca ttaaacgacg
     3361 tcgcttttcg tggacgagtg gttatcggcg aaggagaaat tgaccatgcg ccgatgctgt
     3421 ggattggcga agaggtgggc aaaggcgatg ggccagaagt cgatatcgcg gttgatccca
     3481 ttgaaggcac gcggatggtg gcgatggggc agagcaatgc gctggcggtc atggctttcg
     3541 cccctcgaga tagcctgtta catgcgcccg atatgtatat gaaaaagctg gttgttaatc
     3601 ggctggcggc gagcgcgatt gatctgtctt tgcctctgac ggacaacctg cgcaacgtgg
     3661 cgaaagcgtt aggcaaaccg ctggataagc tgcgcatagt tacgctggat aaaccgcgcc
     3721 tgagtgccgc gattgaggaa gcaacacagc tgggcgtgaa ggtttttgcc ctgccggatg
     3781 gcgatgtcgc cgccagcgtg ctgacctgct ggcaggataa tccttacgat gtgatgtaca
     3841 ccatcggcgg tgcgccagaa ggcgtgattt ctgcctgtgc cgttaaagca ttaggcgggg
     3901 atatgcaggc cgagctgatt gacttttgcc aggctaaagg ggattacacg gaaaatcggc
     3961 agattgcaga gcaggagcgc aagcgttgca aggcaatggg cgttgacgtt aaccgtgtgt
     4021 actcgctcga tgaactggtg agggggaacg atatcctctt tagcgctacg ggcgtgacgg
     4081 gcggtgagct ggtgaacggt atccaacaga cggcgaatgg ggtgcggacg cagacattac
     4141 tgatcggcgg cgcggaccaa acgtgtaata taatagactc cctgcattga tggtgatcta
     4201 tcggaagacc aatggcgacg ctgacagaag atgatgtgct tgagcaactg gatgcacagg
     4261 acaatttatt ttcatttatg aaaactgcgc ataccatttt gctccagggg atacgccagt
     4321 ttctgccgtc gctgtttgtc gataacgatg aagagatcgt ggaatatgca gtgaagccgt
     4381 tactcgccca aagcggcccg cttgacgata ttgatgttgc actgcgtttg atttatgcgt
     4441 tggggaaaat ggataaatgg ttgtacgccg atatcacgca tttttcccag ttctggcatt
     4501 acctgaacga acaggatgaa acgcccggat ttgccgatga catgacctgg gattttatca
     4561 gcaacgtcaa tagcataacc tgcaatgcga cgctctatga cgcgttgaaa gcgatgaagt
     4621 tcgccgattt cgccgtctgg tcagaggcgc gttttagcgg aatggtcaaa acggcgctga
     4681 cgctggcagt aacgaccacc ttaaaggaat taacgccgtg aaaattgaat taacggtgaa
     4741 tgggctgaag atacaggcac agtaccagaa cgaagagatt gaaaatgtac ataaaccgtt
     4801 gctgcacatg ttggcggcct tacagacggt aaatccgcag cggcgtacgg tggtttttct
     4861 ttgcgctccg ccggggacgg gtaaatcaac cctaaccacc ttctgggaat atctcgcgca
     4921 acaagacccg gaactgcccg ctattcagac gctcccgatg gatggttttc accattacaa
     4981 tagctggctg gatgcgcatc aattgcgccc cttcaaaggc gcaccagaga catttgacgt
     5041 tgcgaaactg acggaaaatc tgcgccaggt cgtggaaggg gattgtacgt ggccgcagta
     5101 cgatcgacaa aagcatgatc ctgttgaaga tgcgttgcac gttaccgcac cactcgtcat
     5161 cgtcgaagga aactggttgt tactggatga tgaaaagtgg ctggagttgg catctttctg
     5221 cgatttttct atttttattc atgcacctgc tcaaattctg cgggagcgct tgattagtcg
     5281 taaaattgcg ggaggattaa cgcggcaagt agccgaagca ttttatgccc gcaccgatgg
     5341 ccccaatgtc gagcgtgtgc tgatgaatag ccgtcaggcc aatctgatag tagagatgac
     5401 tgaagaaggc cggtatcact tcacaagctg acaatttatt ccagttattg tagggttatt
     5461 attacgccag cctggaataa agcttcccac aaaaggggat aaaagtgtga tgtgagtcag
     5521 ataaatgtct tcttcggctg gacaaacatt ccttttattc cacgtttcgc ttatcctagc
     5581 tgaagcgttt cagtcgatta aatgttcgac aattaaccaa tcagtcgcag tttgcgacag
     5641 gtaaggtttc cccggacgat ttgctggatt actctgtctg gcaaatttgc tcagggaaaa
     5701 ccttgcagga gatctatgac cgtacgcgta gcgataaatg gcttcggtcg catcgggcgt
     5761 aatgtggttc gtgctttgta tgaatccgga cgccgggcgg aaattaccgt ggtggcaatc
     5821 aacgaactgg cggatgctgc gggcatggcg catttgttga aatatgacac cagccatggc
     5881 cgttttgcat gggaagtacg acaggaacgc gatcaacttt ttgttggtga tgacgccatc
     5941 cgcgtattgc atgaacgttc actgcaatcg ctcccctggc gtgaacttgg cgttgatgta
     6001 gtcctcgact gcaccggcgt atatggctcc cgcgagcatg gcgaagcgca tattgccgcc
     6061 ggggccaaaa aagtgctctt ttcacatcct ggcagtaaca atctcgacgc gaccgttgtt
     6121 tacggcgtca atcaggatca acttcgtgcg gaacaccgca tcgtttctaa cgcttcctgt
     6181 accacgaatt gcataattcc cgtcatcaaa ttgttagatg atgcgtacgg tattgagtcc
     6241 ggcactgtga ccacaattca ctccgccatg cacgatcaac aggttattga tgcataccat
     6301 cctgacctgc gtcgcacccg ggcagccagc cagtcgatca ttccggtcga tactaaactg
     6361 gccgccggta tcacacgatt ttttccgcaa tttaacgatc gctttgaagc gattgcggta
     6421 cgtgtgccaa ccataaatgt gacggcaatc gatttaagcg tgacggtgaa gaaacctgta
     6481 aaagccaatg aagtcaacct gttgctgcaa aaagcagcac aaggtgcatt tcatggtata
     6541 gttgactata cggaattgcc gttggtctct gtagatttta accacgatcc gcacagtgcc
     6601 attgtcgatg gcacccaaac ccgggtcagt ggcgcacacc tgatcaaaac gttggtctgg
     6661 tgcgataacg aatggggctt tgctaaccga atgctcgaca cgacgttagc tatggctact
     6721 gttgctttca ggtaagacgc aagcagcgtc tgcaaaactt ttagaatcaa cgagaggatt
     6781 caccatgtct gtaattaaga tgaccgatct ggatcttgct gggaaacgtg tatttatccg
     6841 tgcggatctg aacgtaccag taaaagacgg gaaagtaacc agcgacgcgc gtatccgtgc
     6901 ttctctgccg accatcgaac tggccctgaa acaaggcgca aaagtgatgg taacttccca
     6961 cctgggtcgt cctaccgaag gcgagtacaa cgaagaattc tctctgctgc cggttgttaa
     7021 ctacctgaaa gacaaactgt ctaacccggt tcgtctggtt aaagattacc tcgacggcgt
     7081 tgacgttgct gaaggtgaac tggttgttct ggaaaacgtt cgcttcaaca aaggcgagaa
     7141 gaaagacgac gaaaccctgt ccaaaaaata cgctgcactg tgtgacgtgt tcgtaatgga
     7201 cgcattcggt actgctcacc gcgcgcaggc ttctactcac ggtatcggta aattcgctga
     7261 cgttgcgtgc gcaggcccgc tgctggcagc cgaactggac gcgctgggta aagcactgaa
     7321 agaacctgct cgcccgatgg tggctatcgt tggtggttct aaagtatcta ccaaactgac
     7381 cgttctggac tccctgtcta aaatcgctga ccagctgatt gttggtggtg gtatcgctaa
     7441 cacctttatc gcggcacaag gccacgatgt gggtaaatcc ctgtacgaag ctgacctggt
     7501 tgacgaagct aaacgtctgc tgaccacctg caacatcccg gttccgtctg atgttcgcgt
     7561 agcaaccgag ttctctgaaa ctgcaccggc taccctgaaa tctgttaacg atgtgaaagc
     7621 tgacgagcag atcctggata tcggtgatgc ttccgctcag gaactggctg aaatcctgaa
     7681 gaatgcgaaa accattctgt ggaacggtcc ggttggcgtg ttcgaattcc cgaacttccg
     7741 caaaggtact gaaatcgtgg ctaacgctat cgcagacagc gaagcgttct ccatcgctgg
     7801 cggcggcgac actctggcag caatcgacct gttcggcatt gctgacaaaa tctcctacat
     7861 ctccactggc ggcggcgcat tcctcgaatt cgtggaaggt aaagtactgc ctgcagtagc
     7921 gatgctcgaa gagcgcgcta agaagtaaaa aatcacaggg cagggaaacc tgcccttgtt
     7981 tcagcgcgct tttagagcac gcaatttttc atactctaaa taattcgagt tgcaggaagg
     8041 cgacaagaga gtgaatcccc aggagcttac ataagtaagt gactggggtg agcgaacgca
     8101 gacgcagcac atgcaacttg aagtatgacg agtataaggc ccgacgatac aggacaagag
     8161 acatgtctaa gatttttgat ttcgtaaaac ctggcgtaat cactggtgat gacgtacaga
     8221 aagttttcca ggtagcaaaa gaaaacaact tcgcactgcc agcagtaaac tgcgtcggta
     8281 ctgactccat caacgccgta ctggaaaccg ctgctaaagt taaagcgccg gttatcgttc
     8341 agttctccaa cggtggtgct tcctttatcg ctggtaaagg cgtgaaatct gacgttccgc
     8401 agggtgctgc tatcctgggc gcgatctctg gtgcgcatca cgttcaccag atggctgaac
     8461 attatggtgt tccggttatc ctgcacactg accactgcgc gaagaaactg ctgccgtgga
     8521 tcgacggtct gttggacgcg ggtgaaaaac acttcgcagc taccggtaag ccgctgttct
     8581 cttctcacat gatcgacctg tctgaagaat ctctgcaaga gaacatcgaa atctgctcta
     8641 aatacctgga gcgcatgtcc aaaatcggca tgactctgga aatcgaactg ggttgcaccg
     8701 gtggtgaaga agacggcgtg gacaacagcc acatggacgc ttctgcactg tacacccagc
     8761 cggaagacgt tgattacgca tacaccgagc tgagcaaaat cagcccgcgt ttcaccatcg
     8821 ctgcgtcctt cggtaacgta cacggtgttt acaagccggg taacgtggtt ctgactccga
     8881 ccatcctgcg tgattctcag gaatatgttt ctaagaaaca caacctgccg cacaacagcc
     8941 tgaacttcgt attccacggt ggttccggtt ctactgctca ggaaatcaaa gactccgtaa
     9001 gctacggcgt agtaaaaatg aacatcgata ccgataccca atgggcaacc tgggaaggcg
     9061 ttctgaacta ctacaaagcg aacgaagctt atctgcaggg gcagttgggt aacccgaaag
     9121 gcgaagatca gccgaacaag aaatactacg atccgcgcgt atggctgcgt gccggtcaga
     9181 cttcgatgat cgctcgtctg gaaaaagcat tccaggaact gaacgcgatc gacgttctgt
     9241 aagatattcc tttctgctta tctcaaggcc cgctctgcgg gtcttttttt cgccaaaagc
     9301 aaaattgcct gatgcgctac gcttatcagg cctacacgta tcctgcaata tattgaattt
     9361 gcaggttttt gtaggccgga taaggcgttc acgccgcatc cggcatgaca aaacgcacat
     9421 tgatatctgt ctgagccccg ctctgcgggg attttttcgc caaaagcaag gtgattcaga
     9481 tgagaaatct gtgatctatt tggcaaaatt atgctttatt gtttaccctt gtcagactgc
     9541 ccgtcataag gcggcggagt gtatttctcc attttgagtc agttgaaaag gaatattgaa
     9601 tggaagattt gaatgttgtc gatagcataa acggcgcggg aagctggctg gtagctaacc
     9661 aggcgctgct gctaagttat gcagtaaaca tcgtggcggc actcgcgatc atcatcgttg
     9721 gtttgattat cgcgcggatg atttccaacg cggtgaatcg cctgatgatc tcccgtaaaa
     9781 tcgatgccac tgttgctgat tttctttctg cattagtccg ttacggtatt atcgccttta
     9841 cgctaatcgc agccctggga cgcgtgggtg tacaaaccgc gtcagtcatt gctgtactcg
     9901 gtgccgcagg cttagctgtt ggtctggctt tgcaggggtc actttctaac ctggccgctg
     9961 gcgtgttact tgtcatgttc cgcccgttcc gtgccggaga atatgttgac ctgggcggcg
    10021 tagccggtac tgtgctgagt gtgcagattt tctccaccac catgcgtact gcagacggta
    10081 aaattatcgt tattccgaac ggtaaaatta ttgccggaaa tattattaac ttctcccgcg
    10141 agccagttcg ccgtaacgaa tttattattg gcgtggcgta tgattccgat atcgatcagg
    10201 ttaagcagat cctgaccaat attatccagt ctgaagatcg cattttgaaa gatcgcgaaa
    10261 tgactgtgcg cctgaacgaa cttggtgcat cgtcgattaa tttcgtggtc cgcgtctgga
    10321 gcaacagcgg cgatctgcaa aacgtgtact gggatgtgct ggagcgtatt aaacgtgaat
    10381 ttgatgccgc cggtatcagc ttcccgtacc cgcaaatgga tgtgaacttt aagcgggtga
    10441 aagaagacaa agctgcgtaa tcaacgctga ggccagataa tactctctgg cctctctctt
    10501 attagttttt ctgattgcca attaatatta tcaatttccg ctaataacaa tcccgcgata
    10561 tagtctctgc atcagatact taattcggaa tatccaacgt gttttcttat tactttcaag
    10621 gtcttgcact tggggcggct atgatcctac cgctcggtcc acaaaatgct tttgtgatga
    10681 atcagggcat acgtcgtcag taccacatta tgattgcctt actttgtgct atcagcgatt
    10741 tggtcctgat ttgcgccggg atttttggtg gcagcgcgtt attgatgcag tcgccgtggt
    10801 tgctggcgct ggtcacctgg ggcggcgtag ccttcttgct gtggtatggt tttggcgctt
    10861 ttaaaacagc aatgagcagt aatattgagt tagccagcgc cgaagtcatg aagcaaggca
    10921 gatggaaaat tatcgccacc atgttggcag tgacctggct gaatccgcat gtttacctgg
    10981 atacttttgt tgtactgggc agccttggcg ggcaacttga tgtggaacca aaacgctggt
    11041 ttgcactcgg gacaattagc gcctctttcc tgtggttctt tggtctggct cttctcgcag
    11101 cctggctggc accgcgtctg cgcacggcaa aagcacagcg cattatcaat ctggttgtgg
    11161 gatgtgttat gtggtttatt gccttgcagc tggcgagaga cggtattgct catgcacaag
    11221 ccttgttcag ttaggcgcta tctgatggaa aaataaaaca gaggcgctaa gcttgcctcc
    11281 agaggtcctg aattttgggc aagtcaatta acacggagag actaacgtga agttcaaagt
    11341 tatcgccctg gcggcattaa tgggtattag cgggatggca gcgcaggcta acgaattgcc
    11401 ggatggaccg catattgtca cctccggtac ggcaagcgtg gatgcggtgc cagacattgc
    11461 cactcttgcg attgaagtta acgtggccgc gaaggatgcc gctactgcca agaaacaggc
    11521 agatgagcgc gtcgcacaat acatttcctt ccttgaactc aatcagatcg cgaaaaaaga
    11581 tatcagctca gcgaacttac gcacccagcc agattatgat tatcaggatg gtaaaagtat
    11641 ccttaaaggc taccgcgctg tgagaacggt ggaagtcacg ctccgtcagt tagacaaact
    11701 gaattccttg ctggatggcg cgctgaaggc gggtcttaac gaaattcgtt ctgtgtcgct
    11761 gggcgtggcg cagccggatg cctataaaga caaagcgcgt aaggcagcga ttgataacgc
    11821 gattcatcag gcgcaggaac tggcgaacgg ctttcatcgt aaactggggc cggtatatag
    11881 cgtgcgctac catgtttcca actatcagcc cagcccaatg gtgcggatga tgaaagccga
    11941 tgccgcgccg gtgtccgccc aggaaactta cgagcaggcc gctattcagt ttgatgatca
    12001 ggtcgatgtg gtcttccagt tagaacctgt ggatcaacaa cccgctaaaa cacctgcagc
    12061 acaataatat ttgtagcccg acgtattcgt cgggctaatg cctttacccg atattgcctc
    12121 ctttttctgt aattttctgt aaaaccatga cgtaatcacc tatctttgct cgcggtttca
    12181 tcaaggatag gctgactgaa acggttaagc ggacaaggat gcgggggagg gggatggaca
    12241 tttttatttc gaaaaagatg cgcaatttca ttttattagc gcaaaccaat aatattgccc
    12301 gggcggcaga aaaaatccat atgacggctt cgccatttgg taaaagcatt accgcgctgg
    12361 aagaacaaat tggctatacg ctatttaccc gcaaagataa taatatcagc ctcaataaag
    12421 ccggacagga actgtatcaa aaactgttcc cggtttatca acggctttct gcgattgata
    12481 atgaaatcca taactcaggg cgtcgttcac gagaaattgt gattggcata gataatactt
    12541 atccgacaat tattttcgat cagttgatta gtctcggaga taagtatgaa ggcgtgaccg
    12601 cccagccagt tgagttcagc gaaaatggcg ttatcgataa tctttttgac cgccagttag
    12661 attttattat ctccccacag catgtatctg cacgagttca ggaactggaa aacctgacta
    12721 tcagtgaact accaccgctg cgattaggct ttctcgtttc tcgccgttat gaagagcgac
    12781 aagagcaaga gctgcttcag gagctgccct ggctacaaat gcgctttcag aaccgggcca
    12841 atttcgaagc aatgattgat gcgaatatgc gaccttgtgg gatcaatcca accattattt
    12901 atcgtcccta cagttttatg gccaaaatca gcgccgtgga gcgcggacat tttctgacgg
    12961 tgatcccgca ttttgcctgg cgactggtga atccggcaac gctgaaatat tttgatgcac
    13021 cgcacaggcc gatgtatatg caggaatatc tttattcaat cagaaatcat cgttataccg
    13081 ccacgatgct tcagcatatt gctgaagatc gtgacgggac gagtcattaa cccagcatcg
    13141 agccggttgc aattaaatta cggtgtaagt cgaagacgtg gctaagatcg tggtgaatat
    13201 gtccgccagg cgcattttcc agatagcgat gcagataatc ccgatacata ggatgtgcac
    13261 aattatcaat gatagtgcgg gcgcgttgaa gcggggaaag accgcgcaga tcggcgatcc
    13321 cttgttcggt gatgatcact ttgacgctgt gttcgctgtg atcaacatgg ctgcacattg
    13381 gcacgacggt tgagatcttg ccttctttag caatcgacgg ggccatcaag atcgacagat
    13441 acgcgttgcg ttcaaaatca ccgctgccgc cgatgccgtt catcagatcg accccggcta
    13501 cgtgtgttga gttggcatgc ccgtaaatat caaactccag gccgacgttc agagcgatga
    13561 cgcccagacg acggatgatt tccgggttat tggaaatctc ctgcggacgc aacacaatgc
    13621 ggctggcaaa gtaatccata ttgtcgtaaa tcttgcgcag ggaatcggcc gagattgtca
    13681 ggctggaggc gctggccccg ctgattttgc cggtttccag taaatgcacc accgattcct
    13741 gtagcacttc cgaatacatc ataaacggag gaattaccgg gttttccccc agacgcgcca
    13801 ttaccgcatt attgatattg cccacgccac tttgcagagg cagaaattcc ggcggaatac
    13861 gcccatgcgc catttcctgc aataagaacg tgaccacgtt atcggcaatc tgctggcaca
    13921 tgggattttg cttatccagc atattaccgg cgtcgggcaa gttggtttcc acgacggcga
    13981 caatcttttt cggatcgatt tgcacatagc gggtaccgac gcgatccatt gcatggaaga
    14041 tcgacacgct attgcgccgc ggtggcgcgc caggaatcac aatatccgcc agttctgcaa
    14101 cgcgcggatc gtgatagtga ttgagttcaa tgatcacttt cttcgcccgc agcagccagg
    14161 tcggcgcatt accgatcccg ctggttaacc agactcgacc atccggtgcc agtgccgatg
    14221 cttcaatgac ggcaacatca atgtcgccga agaaaccgta attgaccatt tgcgccactt
    14281 cgctcaaatg caggtcaacg aaactcaccg cgccctgatt gatcttttta cgtaaaccgg
    14341 acgatgtttg atatggcgca cgccaggaaa cagcatcggc gtcagaaagt acatcgtcag
    14401 cggcggcgct gattgacgca cccgtcagaa ggcgaatttg atacggcttt ttggcctcat
    14461 gctgttcgtt agctctgcgg gcaatcgcgg tgggtagggc tttcggcgaa cccgccgggg
    14521 taaagccgct aaatgccacc atgtcgttat gctggataat ttctgccgct tcattggcgg
    14581 tcatccttgt ccactgagtt tccattttta ctccttcgtt cgcagggatt aatgaccaac
    14641 gaaattaggt ttacgttttt cgaggaaagc gttcatccct tcctggtaat cttcgctgtc
    14701 atacaccgcg cggcgcatcc cctgaatacg ttcaaattca tcggagttca tggtgtgtgc
    14761 ttcgcccagt acacgcagct cttctttgat aacggcaatg gctaacggcg ctttctcaga
    14821 gatgtggtgc gccatttgta aggtgaaatc ttccagttct tccacttcca caacatggtt
    14881 gaggatgccg acagccagcg cgcgctgggc ggtgattggc gaagcggtaa aaatcagctc
    14941 tttgacaatg tggaagcccg cgtcgcgggt caggttgtga atgccgacca ggttatacgg
    15001 gacgccgagg tttacaggcg tcattgagaa ggttgaggta ctggcggcga tgatcagatc
    15061 ggaactcatg atcatttcaa atgcgccacc ccaaacacta ccttccacca tcgaaatgat
    15121 cggtttcggg aatttttgga tcatgcgggt gatttgacgc aatggatcat cataggagag
    15181 cggatcgcga ccgccagacg gcagttcgtg aatatcgtga cctgcggaga agactttgga
    15241 tccactcggt gcgcgcaaaa tgatacagcg aatttccggc cggttgagat cgcttaacgc
    15301 ctgcataaga tcatcaataa agactttact taaggcatta agttttcggc catagttaaa
    15361 ctcaatgacc gccactttgt tgatagtgac aacgttaaca tactgataag acataaaaat
    15421 tcctttaatc aaaatattgc gtctggataa attcactgag ctgccgcagg ccggtgcgcg
    15481 gtgagagcgt attgtttttg accgctaaaa gcgtctggcg gtaatagcga tcgaaatctt
    15541 cattcgcgaa caggtgattc agtacttctt cttcggtctg cttacgcagc cattccaccg
    15601 attgttgttg ccgcacttgt tgtaaacgac cactggcagt tagcgcggtt ttgaagtcga
    15661 tgatggcgtg ccagatctca tcgattccac gtttttccag tgcgctacaa gtcagaaccc
    15721 gtggctgcca ttcgtcgtat ttacgtcgca gaatatgcag ggcactctcg tacatatgcc
    15781 gggcaatggc gacattggta tggttatcgc catcgtcttt gttgataacg atcagatcag
    15841 ccacttccat cagccctttt ttaatgccct gcagatcatc gccgccaccg gcaatttgca
    15901 acgagataaa acagtccacc atgcgggcga cttctgtttc cgactgcccg acgccaaccg
    15961 tttcgacaat cactacgtca taacccgctg cttcgcataa cagcattaat tcccgcgctc
    16021 gctgactggc accgcccaga tgaccggagg atggtaccgg gcgaataaac gccgcttcgg
    16081 cacgcgccag gtcattcatg cgggttttat ccccgagaat gctaccgcca gtgaccgggc
    16141 tgctgggatc gaccgcaata accgcgacct ttaatccctc tcgaatcaac aacatgccaa
    16201 aggcctcaag aaaggtactt ttccccgcgc cgggggtgcc ggtaacgccc agtcgcaggg
    16261 tgttaccgca gtacggcata atggcatcaa gcagctgcgt acttagtgcc tgatgacgcg
    16321 ggtgacggct ttccaccagc gtcatggcct gggcgagtgt ggcacgctca ccctgacgta
    16381 agcggcgaat actttctgcc agcgtggctt cattaatcat gatgctggct tatcagattc
    16441 agtacgtcgc gcacactgtc gagcataggt gtacctggac cataaatcgc cgccacgccg
    16501 cgctcttgca ggaaggcgta atcctgcggc ggaatgacgc cacccgcgac cacgcagata
    16561 tcttcgcgtc cccatttttt cagcgcttcg accagttccg ggatcagcgt tttatgaccg
    16621 gcagccagtg aggatgcgcc cactacgtga acgtcgtttt ctacggccag gcgggcgatc
    16681 tcttcaggtg tagagaacat cgggcttaaa tctacgtcga aaccgagatc ggaataggcg
    16741 ctggcgatca ctttcgcgcc gcgatcgtgt ccatcctggc ccatcttagc gatcagaatg
    16801 cgcgggcgac gaccattgtc ggcaaggaac tgctccgttt gcgcaacaat ggcatcgaac
    16861 tcggaggccg atttctcaga ctgatgatag ctttgcgcaa tcacgccggt aacacactgg
    16921 cttggcacca gataacggtc gaaagcgact tccagcgcat cggaaatttc acccagggtg
    16981 gcgcgaacgc gagcggcatt aacagcggca gccagcaggt tttcgttatg ctgtgcggcg
    17041 tgagtcaggg cgttcaacgc ggcggttacg gcggcatcat cacgggtggc gcgaatgcgt
    17101 tccagcgaag caatttgctc gttacgcacc atcacgttgt cgatctcaag tacatcggtt
    17161 tcgtcttcgt gatccagttt gtacttgttg acaccaacga tgacacgctt gccctggtcg
    17221 atcagcgact gttcgcgcgc tgaggcctct tcgatcattc gttttggcag acctgcttcg
    17281 atcgctttcg ccatgccacc ggcttcgtcg atctgttgga taatagctct ggcttgtttg
    17341 acgatttgat cggtcagcga ctcaatgtaa taggatccgg ccagtggatc gacggtgcgg
    17401 cagagttctg attcttcctg gatgatgatc tgggtgttgc gggcaatgcg tgctgagaaa
    17461 tcggtaggca aaccaagcgc ttcgtcaaag gcgttggtat gcagtgactg agtaccgccc
    17521 agcgtcgcag ccagcgcttc aatggtggtg cggataacgt tgttatacgg atcctgttca
    17581 gtcaggctcc agcctgaggt ctggcagtgg gtacgcagcg ccagtgattt cgggtcctgt
    17641 gcgccaaatc cactgaccgc ttcgctccat aaataacgtg ccgcacgcaa catggcgacg
    17701 ttcataaaca gatccatgcc gatgccgaag aagaacgaca ggcgaggagc gaagtcatca
    17761 attttcagtc cggcagagat tgctgctttg atgtactcaa tcccatcagc gagcgtaaat
    17821 gctacctgct gcacgcagtt ggcacccgct tcacccatgt ggtaaccgct gatactgatg
    17881 gtattaaatc gcggcatgtt gccggaacac caggcgatga tgtcggcgat aatgcgcatt
    17941 gacggttttg gtgggtaaat ataggtgttg cggcagaggt actctttgag aatatcgttt
    18001 tgaatggtgc cggtcagttt atcaggtgta acaccttgct cttctgcggc gacgatataa
    18061 aacgccagta ctggtagcac tgcgccattc atggtcatcg aaaccgacat tttatccagc
    18121 gggatctggt cgaacaggac tttcatatct tccacggtgt cgatagcgac gcccgctttg
    18181 ccgacgtcgc ccgccacgcg cgggttatcg gagtcgtagc cacggtgggt ggcaaggtca
    18241 aacgcaacgg aaagaccttt ttgcccggcg gccaggttac ggcgataaaa agcgttggac
    18301 tcttttgctg ttgaaaaacc agcatactga cggatggtcc acggttgggc ggtatacata
    18361 gtggcacgcg ggccacgaac gtagggcggc aaaccaggaa gggtacctgt cacctccaga
    18421 ttatcgagat cggcttcggt atacagcggc ttgatggcga tcccttccgc ggtttgatga
    18481 accagcgagt cgacagtttt ctcccgacgg ctcaattcct tgttggcaag ctgttgccac
    18541 tcctgcacgt tagacattat ttgctccgta aaacgcaatc aaaatgaggg tatttgcgcc
    18601 tacggtgagc tttttacgtg ggtttgtcgc cagcaaaaaa aatgcattca gacgtcgttt
    18661 tttgccgcct gagatttgtc tattttgtga tgtgattcaa ggaatacaaa aagaagtgag
    18721 gacaacgcct gatatgtgct atcaggcatt atttgatgga ttaatcctga cgaaggactt
    18781 tgtgaccata atcgagtaac gcatcagtga ctttacgcat catgcggctt tcaggagcaa
    18841 agcggtgcca gtagagcatc cgtcgttgaa atagcccagg cgttaagtca atcagttcac
    18901 cgctggccag ctctttctcg atttgcaggt gcgggatcat acagcaggtg gtgccctggc
    18961 gagcaagttg tacgaacgct tctgaagaat taacgatatg gcagggcacg ctgcctggag
    19021 gcagatcgaa gttttgctgc aaaaaggcct ggtgcatatc gtcaagatgg tcaaacgcga
    19081 ccactggcgc tttcagtaat gccgaacgcg ttacgccgtt agggaaatat ttttcggcaa
    19141 agggttttga gctgacgaac agatagtcga gcgcaccaag tttatcgaca agacaactcg
    19201 gcagcgcctg atgttgaata ctcaccgcgc cgaccacttc gccgcggcgc agacgttcct
    19261 gagtgcgggt ttcatcttct acctgcaagt tgaggcggat aggcgaatca gccaacacag
    19321 gagccagtgc aggaagcaac cacgtcgcca gactgtcggc gttgaccgcc agtgaaagca
    19381 gcagcggagt cgaaccggtt tgttcatcgc ccagccactc ttcttccagc aactccacct
    19441 ggcgcagcag tgccagcagt ttttgccctt gttccgtcgg gcgcggcggt acggtacgca
    19501 ccaacagcgg ctgcccgaac atattttcca gttgcttaat gcgctgtgag acggctgatt
    19561 gtgtaatgca cagcttttgt gccgcgcgct caaatcctcg ttcacgtatc accgcatcca
    19621 gtgcctgtaa tgttctgtag tccgggcgtt tcattgctgt tgtctgctcc tggtaaatgc
    19681 tttttcctgc actatgacac aattttgtgt caggttgcat ataccgaagc cgtcggcggg
    19741 taacatttgt attcgtggtt aaacgcgcag cttaaaagat cagggatttg cgctaatgca
    19801 tttcgctcag cgagttcgcg ccctggttgt gttgaatggc gtggcgttat tgcctcaatt
    19861 tgcctgtaaa caggggcttg tgaacggtga actggtgcgc ctgtttgctc cgtggagcgg
    19921 catacccaga ttgttgcatg ccttatttgc ggggcgaaag gggatgcctg ccattgcgcg
    19981 atattttatg gatgagttaa ccacgcggct tgccaacggg gtctgaatcg ctttttttgt
    20041 atataatgcg tgtgaaattt cataccacag gcgaaacgat catgacgcag gatgaattga
    20101 aaaaagcagt aggatgggcg gcacttcagt atgttcagcc cggcaccatt gttggtgtag
    20161 gtacaggttc caccgccgca cactttattg acgcgctcgg tacaatgaaa ggccagattg
    20221 aaggggccgt ttccagttca gatgcttcca ctgaaaaact gaaaagcctc ggcattcacg
    20281 tttttgatct caacgaagtc gacagccttg gcatctacgt tgatggcgca gatgaaatca
    20341 acggccacat gcaaatgatc aaaggcggcg gcgcggcgct gacccgtgaa aaaatcattg
    20401 cttcggttgc agaaaaattt atctgtattg cagacgcttc caagcaggtt gatattctgg
    20461 gtaaattccc gctgccagta gaagttatcc cgatggcacg tagtgcagtg gcgcgccagc
    20521 tggtgaaact gggcggtcgt ccggaatacc gtcagggcgt ggtgaccgat aatggcaacg
    20581 tgatcctcga cgtccacggc atggaaatcc ttgacccgat agcgatggaa aacgccataa
    20641 atgcgattcc tggcgtggtg actgttggct tgtttgctaa ccgtggcgcg gacgttgcgc
    20701 tgattggcac acctgacggt gtcaaaacca ttgtgaaatg atctgacggg ggaacctccc
    20761 ccgttaaaaa aattctcttc attaaatttg gtgacatgtg tcacgttttt acctggctat
    20821 tgtcgattgc tctaaataaa tcttctaaac cagcatattc atccaagaat tacctttgcg
    20881 tgatatttcc tcaacatcgc gacgcaaacg ttcatattgc cgcaatatta ttttttgata
    20941 tgttgaaagg cggacgcaaa tccgcacaca acatttcaaa agacaggatt gggtaaatgg
    21001 caaaggtatc gctggagaaa gacaagatta agtttctgct ggtagaaggc gtgcaccaaa
    21061 aggcgctgga aagccttcgt gcagctggtt acaccaacat cgaatttcac aaaggcgcgc
    21121 tggatgatga acaattaaaa gaatccatcc gcgatgccca cttcatcggc ctgcgatccc
    21181 gtacccatct gactgaagac gtgatcaacg ccgcagaaaa actggtcgct attggctgtt
    21241 tctgtatcgg aacaaaccag gttgatctgg atgcggcggc aaagcgcggg atcccggtat
    21301 ttaacgcacc gttctcaaat acgcgctctg ttgcggagct ggtgattggc gaactgctgc
    21361 tgctattgcg cggcgtgccg gaagccaatg ctaaagcgca ccgtggcgtg tggaacaaac
    21421 tggcggcggg ttcttttgaa gcgcgcggca aaaagctggg tatcatcggc tacggtcata
    21481 ttggtacgca attgggcatt ctggctgaat cgctgggaat gtatgtttac ttttatgata
    21541 ttgaaaataa actgccgctg ggcaacgcca ctcaggtaca gcatctttct gacctgctga
    21601 atatgagcga tgtggtgagt ctgcatgtac cagagaatcc gtccaccaaa aatatgatgg
    21661 gcgcgaaaga aatttcacta atgaagcccg gctcgctgct gattaatgct tcgcgcggta
    21721 ctgtggtgga tattccggcg ctgtgtgatg cgctggcgag caaacatctg gcgggggcgg
    21781 caatcgacgt attcccgacg gaaccggcga ccaatagcga tccatttacc tctccgctgt
    21841 gtgaattcga caacgtcctt ctgacgccac acattggcgg ttcgactcag gaagcgcagg
    21901 agaatatcgg cctggaagtt gcgggtaaat tgatcaagta ttctgacaat ggctcaacgc
    21961 tctctgcggt gaacttcccg gaagtctcgc tgccactgca cggtgggcgt cgtctgatgc
    22021 acatccacga aaaccgtccg ggcgtgctaa ctgcgctcaa caaaattttt gccgagcagg
    22081 gcgtcaacat cgccgcgcaa tatctgcaaa cttccgccca gatgggttat gtggttattg
    22141 atattgaagc cgacgaagac gttgccgaaa aagcgctgca ggcaatgaaa gctattccgg
    22201 gtaccattcg cgcccgtctg ctgtactaat tccccttctc tgaaaatcaa cgggcaggtc
    22261 actgacttgc ccgttttttt atcccttctc cacaccgttg ccgcgttgcg cattctactt
    22321 tgcgagtctg ctccagaaaa taaatcaaag tcagatcaat gcgttgtgtt tatttaatgg
    22381 ggatttacga tggcagggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg
    22441 cttgaaggag aagggttatg atgcgacttg tcatcatact gattgtactg ttactcataa
    22501 gtttcagcgc ttattaacag tcagtctcag gggaggagca atcctccctt acccttactc
    22561 actaaattag gtcaaagaat caacgatgtc aatcagggcg atgcaggtgt gtcgccctta
    22621 ccactcccag actttcgacg gtgtaaccac cgcaggcagt gggatatccc actcttcaac
    22681 ggggagtttt tccaccaact gacaatcatg cgcataaccc accggttgca ttttatagtg
    22741 ctgccagttt tgtaaggtcc gatcataaaa acctccgccc attcccaggc gctgaccgta
    22801 ctcatcaaag gcgaccaggg gggtgatcag gacgtctaat cgggaaaggg ggagcacgtc
    22861 acgcacatcc aattttggct catggatctt caacctgttc atcaccagtt cgctttgcgg
    22921 atggtaattc aggaacagca aattaccggc actaaaggga tgcaaaactg gaagatatac
    22981 gcgcttaccg gcgcgccaga gttgttctat gagtggctgg gtgtcgagtt cgccatcaaa
    23041 agagaggaat acagcgaccg tatgtgccat caccaccggg ggataagtca tcatccgggt
    23101 agcggcttgt tgacccattt cctgctgttg ttccggcgtt aacgcacgac gacgttgccg
    23161 aatcattttg cggatttctt gtcgggataa tatcagtggg agttctggta gttgcgtcat
    23221 ggctggtgcc agataagaag ggaatctccg agatgccgcc gcaggctgta acccttgaac
    23281 ccttggttca aggtgaatgt gtcgtcgcag ttttaaggct tctcggacgg accgagcatg
    23341 ctcaccaacc gcggagcgcc acattcttgt ggtatgaaat atcggctcag gggactggcc
    23401 cgcttgcgaa catctcagag aaattttgtc ttcacggtta ctctaccaca gtaaaccgaa
    23461 aagtgtcatt caaagttttg gttagttttt tcggtgatgc gaccttgttc aagtaacgct
    23521 tgttctatgg tctgctgcag catccgaata cgctgttcca tacttgccgc gtagtcacga
    23581 gtcttcgctt tttcttgcgc taactcatag ctgatattca atgcggcaat gaagaccaac
    23641 tgttcagtat ttgtgactct agtgcgttct ttcagatctt gcaaccgttg gttcagatcg
    23701 tccgctgcct gattcaacgc atccctttgg tcaggcgggc agttcacacg cagtgaacgg
    23761 ccaaaaattt ggatatcgac gggttgtgca gacatgccac cttcctgctg attgactgcg
    23821 ctgccttcgt ctttagaccc atggtctgcg aaggggcgac actatagcta ccctgatgag
    23881 aagagacaag cccttttctg gtccaccagg gcccaaagtg gtagcatatc atgaatattc
    23941 ctccctttga cgacgaatgc ttatgtctat acagaacgaa atgcctggtt acaacgaaat
    24001 gaaccagtat ctgaaccaac aagggacggg tctgacccca gctgagatgc atggtttaat
    24061 cagcgggatg atatgtggcg gtaacgatga cagctcatgg ctaccgctac ttcacgacct
    24121 gacgaacgaa ggcatggctt tcggtcatga gctggcacag gcactgcgta aaatgcactc
    24181 tgccaccagc gatgccctgc aggatgacgg cttccttttt cagctttatc tgcctgatgg
    24241 cgatgatgtc agcgttttcg atcgggctga tgcattggca ggttgggtca atcacttcct
    24301 gcttggtctt ggcgttacgc aaccgaagct ggataaagtg accggcgaaa ccggtgaagc
    24361 tatcgacgat ctgcgtaaca ttgcgcaact gggttacgac gaagacgaag atcaggaaga
    24421 gcttgaaatg tcgcttgaag agatcatcga atacgttcgt gttgccgcgc tgttatgcca
    24481 cgacaccttt actcatccgc aaccgaccgc gccagaagta caaaaaccga ctctacacta
    24541 aaaacaaaaa acgtaaggag agtgttatga gtgagatatc ccggcaagag tttcagcgtc
    24601 gccgtcaggc cctggtggag caaatgcaac ccggcagcgc cgcgctgatt tttgctgcac
    24661 cagaagtaac acgtagcgcc gacagcgaat acccctatcg tcagaacagt gacttctggt
    24721 acttcaccgg ctttaacgaa ccggaagcgg tgctggtgct gattaaaagc gatgacactc
    24781 ataaccacag cgttctgttt aaccgcgttc gcgacctgac ggcggagatc tggtttggcc
    24841 gtcgcttagg ccaggatgcc gcgccagaga aactgggcgt tgaccgcgca ctggcattca
    24901 gcgaaatcaa tcagcaactt tatcaactac ttaacggcct ggatgtggtt taccatgccc
    24961 agggcgaata tgcatatgct gatgaaatcg tgaacagtgc gctggaaaaa ctgcgtaaag
    25021 gttcgcggca aaatctcacc gcaccggcaa cgatgatcga ctggcgtcct gttgttcatg
    25081 aaatgcgcct gttcaaatcg ccagaagaga ttgccgtact ccgccgcgcg ggagaaatca
    25141 ccgccatggc acatacccgg gcgatggaaa aatgccgtcc gggaatgttc gagtaccatc
    25201 tggaaggcga aattcaccac gaatttaacc gccacggtgc gcgctatccg tcctataaca
    25261 ccattgtcgg cagcggtgaa aacggctgca ttctgcacta caccgaaaac gagtgtgaaa
    25321 tgcgcgacgg cgacctggtg ttgattgacg cgggttgcga atacaaaggt tacgctggcg
    25381 atattacccg caccttcccg gtcaacggca aattcaccca ggcccagcgt gaaatctacg
    25441 acattgtgct ggagtctctc gaaaccagcc tgcgcctgta tcgtccggga acttccattc
    25501 tggaagtcac tggtgaagtg gtgcgcatca tggttagcgg cctggtaaaa ctcggcatcc
    25561 tgaaaggtga tgttgatgaa ctgatcgctc agaacgccca tcgtcctttc tttatgcatg
    25621 gccttagcca ctggttagga ctggatgtcc atgacgttgg cgtttatggt caggatcgct
    25681 cgcgcattct ggaaccgggg atggtactga ccgtagagcc tggtctgtat atcgcgccgg
    25741 atgcggatgt gccagaacaa tatcgcggta tcggcattcg tattgaagac gacattgtga
    25801 ttaccgaaac cggtaacgaa aacctcaccg ccagcgtggt gaaaaagccg gaagaaatcg
    25861 aagcgttgat ggctgctgcg agaaagcaat gagcgtaatc atcgtcggtg gcggcatggc
    25921 gggcgcgacg ctggcgctgg ctatttcccg gttaagtcac ggggcgctgc cggtacattt
    25981 gattgaagcg actgcgccag agtcacatgc tcatccgggc tttgatggac gagcgatagc
    26041 gctggcggcg ggtacctgtc agcaactggc gcgcatcggc gtctggcaat ctctggcgga
    26101 ttgcgcaact gccatcacca ccgtgcatgt cagcgatcgt ggtcacgctg gatttgtcac
    26161 cctcgccgca gaagattacc aactggcggc gctgggacag gttgtcgaat tgcacaatgt
    26221 cgggcaacgg ctgtttgcat tgctgcgtaa agcacctggc gtaacgctgc attgccctga
    26281 tcgcgtggct aacgttgccc gtactcagag tcacgttgaa gtgacgctgg agagtggcga
    26341 gacgctgacg ggccgcgtgc tggtagccgc tgatggcacc cattcagcgt tagccaccgc
    26401 gtgcggcgtt gactggcagc aggagcctta cgaacaactg gcagtgattg ccaacgttgc
    26461 tacttctgtt gcgcacgaag gccgcgcttt tgaacgcttc acgcaacatg gcccgctggc
    26521 gatgttgccg atgtctgacg gacgctgttc gctggtctgg tgtcatccac tggaacggcg
    26581 cgaagaggtg ctgtcgtgga gtgacgagaa gttttgccgt gagctccagt cggcctttgg
    26641 ctggcgactt ggaaaaatta cccacgccgg taaacgcagt gcttatccgc tggcgttaac
    26701 ccgcgccgcc agagctatta cccatcgtac cgtgctggtg ggcaatgcgg cgcaaaccct
    26761 gcacccgatt gccgggcaag ggttcaacct cggtatgcga gatgtgatga gtcttgcgga
    26821 aaccctgact caggcgcagg agcgcggaga agacatgggg gattacggcg tattgtgccg
    26881 ttatcagcag cgtcgacaga gcgatcgcga agcaaccatc ggcgtcacgg acagcctggt
    26941 acatcttttt gccaaccgtt gggcaccgct ggttgtcggg cgcaacatcg gactgatgac
    27001 gatggaatta ttcaccccgg cacgcgatgt gctggcgcag cgcaccctcg gttgggtggc
    27061 gcgttgaagg cggttttaag gaataaacaa tgcaaagtgt tgatgtagcc attgttggtg
    27121 gcggcatggt ggggctggcg gttgcctgcg gcttacaggg gagcggctta cgcgttgccg
    27181 tactggagca gcgcgtaccg gaacctctgg cggcggatgc accaccacaa ctgcgcgttt
    27241 cggctatcaa tgccgccagc gaaaaattac tcacccgtct tggcgtctgg caggacattc
    27301 tctctcgtag ggccagttgt tatcacggta tggaagtgtg ggacaaagac agttttggtc
    27361 acatttcgtt tgacgatcaa agcatgggct atagccatct tgggcatatc gttgaaaact
    27421 cagtgattca ctacgcgctg tggaacaaag cgcagcagtc gtcagatatc actctgttgg
    27481 cccccgcaga attacagcag gtcgcctggg gagaaaatga aactttcctg acgctgaaag
    27541 atggcagcat gttaacggcg cgtctggtga ttggcgcgga cggtgctaat tcctggttgc
    27601 gcaacaaagc agatattccg ctgactttct gggattatca gcatcacgcg ctggtagcga
    27661 ccattcgcac ggaagaaccg catgatgcgg tggcgcggca ggttttccat ggcgaaggca
    27721 ttctggcctt tttaccgctt agcgatccgc atctttgctc aattgtctgg tcactgtcgc
    27781 cagaggaagc gcagcggatg cagcaggcaa gtgaagatga atttaatcgc gcgttaaata
    27841 tcgcttttga taatcgcctg ggcttatgca aggttgagag cgcgcgtcag gtgttcccac
    27901 tgacggggcg ttatgcgcgc cagtttgccg cgcaccgtct ggtgctggtg ggcgacgccg
    27961 cgcataccat tcacccgctg gcggggcagg gagtaaatct cggctttatg gatgctgcag
    28021 agctgattgc tgaactgaaa cggttgcatc gtcaggggaa agacatcggg cagtacattt
    28081 acctgcgccg gtatgagcgt agccgcaagc acagtgcggc gctgatgctg gctggtatgc
    28141 agggattccg cgatctgttt tccggtacca atccggcgaa aaaactgctg cgtgatattg
    28201 gtttgaaact ggccgacacg cttcctggcg ttaaaccaca acttatccgc caggcaatgg
    28261 gattaaacga tttgcctgaa tggctgcgtt aaaaatttct cttctgctgt ttatttgata
    28321 cccatcacac tttcatctcc cggttttttc gccgggagat tttcctcatt tgaaataaac
    28381 taatttcacc tccgttttcg cattatattt tctaatgtca ttattttttg atttagtgtt
    28441 ttttgacatt tttttagctc ttaatattgc cttattcaaa ttagctttct aatggcatca
    28501 tctttatata gaaactggtg tgttttttgg ttttttattc tgtcgcgatt ttggcgtttt
    28561 ttaaccataa gctaatgtga tgatcaattt taccttatgg ttaacagtct gtttcggtgg
    28621 taagttcagg caaaagagaa cgattgcgtt ggggaccggg agtggctccg atgctgggtt
    28681 tcgtggtgat aatttcacca tgaaaagttg tcagccccgc ttattcaatg aggacaagat
    28741 ggcacaacag actcctttgt acgaacaaca cacgctttgc ggcgctcgca tggtggattt
    28801 ccacggctgg atgatgccgc tgcattacgg ttcgcaaatc gacgaacatc atgcggtacg
    28861 taccgatgcc ggaatgtttg atgtgtcaca tatgaccatc gtcgatcttc gcggcagccg
    28921 cacccgggag tttctgcgtt atctgctggc gaacgatgtg gcgaagctca ccaaaagcgg
    28981 caaagccctt tactcgggga tgttgaatgc ctctggcggt gtgatagatg acctcatcgt
    29041 ctactacttt actgaagatt tcttccgcct cgttgttaac tccgccaccc gcgaaaaaga
    29101 cctctcctgg attacccaac acgctgaacc tttcggcatc gaaattaccg ttcgtgatga
    29161 cctttccatg attgccgtgc aagggccgaa tgcgcaggca aaagctgcca cactgtttaa
    29221 tgacgcccag cgtcaggcgg tggaagggat gaaaccgttc tttggcgtgc aggcgggcga
    29281 tctgtttatt gccaccactg gttataccgg tgaagcgggc tatgaaattg cgctgcccaa
    29341 tgaaaaagcg gccgatttct ggcgtgcgct ggtggaagcg ggtgttaagc catgtggctt
    29401 gggcgcgcgt gacacgctgc gtctggaagc gggcatgaat ctttatggtc aggagatgga
    29461 cgaaaccatc tctcctttag ccgccaacat gggctggact atcgcctggg aaccggcaga
    29521 tcgtgacttt atcggtcgtg aagccctgga agtgcagcgt gagcatggta cagaaaaact
    29581 ggttggtctg gtgatgaccg aaaaaggcgt gctgcgtaat gaactgccgg tacgctttac
    29641 cgatgcgcag ggcaaccagc atgaaggcat tatcaccagc ggtactttct ccccgacgct
    29701 gggttacagc attgcgctgg cgcgcgtgcc ggaaggtatt ggcgaaacgg cgattgtgca
    29761 aattcgcaac cgtgaaatgc cggttaaagt gacaaaacct gtttttgtgc gtaacggcaa
    29821 agccgtcgcg tgatttactt tttggagatt gattgatgag caacgtacca gcagaactga
    29881 aatacagcaa agaacacgaa tggctgcgta aagaagccga cggcacttac accgttggta
    29941 ttaccgaaca tgctcaggag ctgttaggcg atatggtgtt tgttgacctg ccggaagtgg
    30001 gcgcaacggt tagcgcgggc gatgactgcg cggttgccga atcagtaaaa gcggcgtcag
    30061 acatttatgc gccagtaagc ggtgaaatcg tggcggtaaa cgacgcactg agcgattccc
    30121 cggaactggt gaacagcgaa ccgtatgcag gtggctggat ctttaaaatc aaagccagcg
    30181 atgaaagcga actggaatca ctgctggatg cgaccacata cgaagcattg ttagaagacg
    30241 agtaacggct ttattcctct tctgcgggag aggatcaggg tgaggaaatt tacgcctcac
    30301 cctcactctc ttcgtaagga gagaggttca caattcactg cacgtttcag gaaccatcgc
    30361 tcatgacaca gacgttaagc cagcttgaaa acagcggcgc ttttattgaa cgccatatcg
    30421 gaccggacgc cgcgcaacag caagaaatgc tgaatgccgt tggcgcacaa tcgttaaacg
    30481 cgctgaccgg ccagattgta ccgaaagata ttcagcttgc gaccccgccg caggttggcg
    30541 caccggcgac cgaatacgcc gcgctggcgg aactcaaggc tattgccagt cgcaataaac
    30601 gcttcacgtc ttacatcggc atgggttaca ccgccgtgca gctaccgccg gttatcctgc
    30661 gtaacatgct ggaaaatccg ggctggtata ccgcgtatac tccgtatcaa cctgaagtct
    30721 cccagggccg ccttgaagca ctgctcaact tccagcaggt aacgctggat ttaaccgggc
    30781 tggatatggc ctctgcttct cttctggacg aggccaccgc tgccgccgaa gcaatggcga
    30841 tggcgaaacg cgtcagcaaa ctgaaaaatg ccaaccgctt cttcgtggct tccgatgtgc
    30901 atccgcaaac gctggatgtg gtccgtactc gtgccgaaac ctttggtttt gaagtgattg
    30961 tcgatgacgc gcaaaaagtg ctcgaccatc aggacgtctt cggcgtgctg ttacagcagg
    31021 taggcactac cggtgaaatt cacgactaca ctgcgcttat tagcgaactg aaatcacgca
    31081 aaattgtggt cagcgttgcc gccgatatta tggcgctggt gctgttaact gcgccgggta
    31141 aacagggcgc ggatattgtt tttggttcgg cgcaacgctt cggcgtgccg atgggctacg
    31201 gtggcccaca cgcggcattc tttgcggcga aagatgaata caaacgctca atgccgggcc
    31261 gtattatcgg tgtatcgaaa gatgcagctg gcaacaccgc tctgcgcatg gcgatgcaga
    31321 ctcgcgagca acatattcgt cgtgagaaag cgaactccaa catttgtact tcccaggtac
    31381 tgctggcaaa catcgccagc ctgtatgccg tttatcacgg cccggttggc ctgaaacgta
    31441 tcgctaaccg cattcaccgt ctgaccgata tcctggcggc aggcctgcaa caaaaaggtc
    31501 tgaagctgcg ccatgcgcac tatttcgaca ccttgtgtgt ggaagtggcc gacaaagcgg
    31561 gcgtactgac gcgtgccgaa gcggctgaaa tcaacctgcg tagcgatatt ctgaacgcgg
    31621 ttgggatcac ccttgatgaa accaccacgc gtgaaaacgt aatgcagctt ttcaacgtgc
    31681 tgctgggcga taaccacggc ctggacatcg acacgctgga caaagacgtg gctcacgaca
    31741 gccgctctat tcaacctgcg atgctgcgcg acgacgaaat cctcacccat ccggtgttta
    31801 atcgctacca cagcgaaacc gaaatgatgc gctatatgca ctcgctggag cgtaaagatc
    31861 tggcgctgaa tcaggcgatg atcccgctgg gttcctgcac catgaaactg aacgccgccg
    31921 ccgagatgat cccaatcacc tggccggaat ttgccgaact gcacccgttc tgcccgccgg
    31981 agcaggccga aggttatcag cagatgattg cgcagctggc tgactggctg gtgaaactga
    32041 ccggttacga cgccgtttgt atgcagccga actctggcgc acagggcgaa tacgcgggcc
    32101 tgctggcgat tcgtcattat catgaaagcc gcaacgaagg gcatcgcgat atctgcctga
    32161 tcccggcttc tgcgcacgga actaaccccg cttctgcaca tatggcagga atgcaggtgg
    32221 tggttgtggc gtgtgataaa aacggcaaca tcgatctgac tgatctgcgc gcgaaagcgg
    32281 aacaggcggg cgataacctc tcctgtatca tggtgactta tccttctacc cacggcgtgt
    32341 atgaagaaac gatccgtgaa gtgtgtgaag tcgtgcatca gttcggcggt caggtttacc
    32401 ttgatggcgc gaacatgaac gcccaggttg gcatcacctc accgggcttt attggcgcag
    32461 acgtttcaca ccttaacctg cataaaactt tctgcattcc gcacggcggt ggtggtccgg
    32521 gtatgggacc gatcggcgtg aaagcgcatc tggcaccgtt tgtaccgggt catagcgtgg
    32581 tgcaaatcga aggcatgtta acccgtcagg gcgcggtttc tgcggcaccg ttcggtagcg
    32641 cctctatcct gccaatcagc tggatgtaca tccgcatgat gggcgcagaa gggctgaaaa
    32701 aagcaagcca ggtggcaatt cttaacgcca actatattgc cagccgcctg caggatgcct
    32761 tcccggtgct gtataccggt cgcgacggtc gcgtggcgca cgaatgtatt ctcgatattc
    32821 gcccgctgaa agaagaaacc ggcatcagcg agctggatat tgccaagcgc ctgatcgact
    32881 acggtttcca cgcgccgacg atgtcgttcc cggtggcggg tacgctgatg gttgaaccga
    32941 ctgaatctga aagcaaagtg gaactggatc gctttatcga cgcgatgctg gctatccgcg
    33001 cagaaatcga ccaggttaaa gccggtgtct ggccgctgga agataacccg ctggtgaacg
    33061 cgccgcacat tcagaacgaa ctggtcgccg agtgggcgca tccgtacagc cgtgaagttg
    33121 cagtattccc ggcaggtgtg gcagacaaat actggccgac ggtgaaacgc ctggatgatg
    33181 tttacggcga ccgtaacctg ttctgctcct gcgtaccgat tagcgaatac cagtaattca
    33241 ctgattcgac tatcttctaa aggcgcttcg gcgccttttt agtcagatga caaagtacaa
    33301 aagcgctcag acagtcccct cacccctttg gggagagggt tagggtgagg ggaacaggcc
    33361 ggcactggcg cgaatattta ccctcacccc ggccctctcc ctgaaagggc gaggggaaaa
    33421 gcgtgccaac attgaagatt gagccagttt gttagcaatc tcaaagatac gtcaacgaat
    33481 taatttttct cggaaaaaca aatggctata gcacttgtga ccggtggcag tcgcggcatc
    33541 gggcgggcaa ctgcattact gttggcgaaa gaagagtata cggtggcggt taattatcaa
    33601 caaaacctcc atgcggcgca ggaagtggtg aacttaatca cgcaagccgg tggcaaggca
    33661 ttcgtgctcc aggcggatat cagcgacgaa aatcaggtca tcgcaatgtt tacagcaatc
    33721 gatcagcacg atgaaccgct agcagcgctg gtcaataacg ccgggatctt gtttacccag
    33781 tgcaccgttg aaaaccttac cgcagagcga atcaaccgag tactttccac caacgtgacg
    33841 ggatattttc tctgctgccg cgaggcggta aaacgcatgg cgcttaaaaa tggtggcagt
    33901 ggcggcgcta tcgtcaatgt ctcttcggtg gcctcacggt tgggttcgcc aggggaatat
    33961 gttgattacg cggcatcgaa aggggcgatt gatacgttaa ccaccggact atcgctggaa
    34021 gtcgccgcgc aggggatccg cgttaactgc gtgcggccag ggtttattta taccgaaatg
    34081 cacgccagcg gcggcgagcc tggacgcgtc gatcgcgtta agtcgaacat ccccatgcag
    34141 cgtggtggac aggcagaaga ggtcgcgcag gccattgtct ggctactaag tgataaagcc
    34201 tcttacgtca cgggaagttt tatcgatttg gcgggcggga aataaaacag ggaagttgtc
    34261 tgaccggatg caacaagtat tgcatccggt acttcatcga cttaaagctt ctcgccgttg
    34321 ctggcaatca cctctttgta ccagttaaag ctcttcttac gtgaacgcga catatcgcca
    34381 gtaccgtcgt catgtttatt cacatagata aagccgtagc gtttgctgta ctgcccggta
    34441 gtgaatgaca cgcagtcgat gcagccccac ggtgtgtagc ccatcagatc cacaccatcg
    34501 taagtcactg cttttttcat ctcttcaata tgggcgcgca ggtagtcaat gcggtagtcg
    34561 tcgttgatgc tgccatcttc ttccactttg tcgtaagcgc caaaaccgtt ttcgacaata
    34621 aacagcggcc tctgataacg ctcatacagt tcgcaaaggg catagcgcag acctactgga
    34681 tcaatctgcc agccccagtc agacgcttta acatacgggt tcggtacgct gccttcaaaa
    34741 ccagagatcg catcgccggt gccgccttcg gccttcactg cgttggtcat gtaatagctg
    34801 aaaccaagat aatcgcaggt gccttcacgc agtacatcca gatcgccatc ttccattttg
    34861 atgttaaatc cgcgacgctc ccactcgttc aacacatagg acgggtaata gccgcgtagc
    34921 tgcacatcgg taaagacgta gcgttcgcgc atcgactcct gagcgaacat cacatcgtcc
    34981 gggttacagg agtagggata gagcggcacc atcgccagca tacagccgac tttcatctcc
    35041 gggttaatgc gacgcgcggc tttcactgcc agggcgctgg cgacaaactg gtgatgcagc
    35101 acctggtaca tcgtctcttc cgggttgtca tgctcggtat acaccacgcc ggagcagcag
    35161 taaccaaaca gcggcgcacg ccagttacgc tggttgttta tttcgttgaa ggtcatccag
    35221 tatttgactt tatgcttgta gcgttcgaat accacttccg cgaaacggac aaagaaatca
    35281 acgactttac ggttggtcca gctaccgtat tgctgcacca gatgcagcgg catttcaaag
    35341 tgggagaggg ttatcaccgg ttcgatgttg tatttcagca gttcatcgaa catatcatcg
    35401 tagaacttca gcccttcttc gtttggctga gcttcatcac cttttggaaa aatacgcgtc
    35461 caggcgatgg aagtccgaaa acatttgaag cccatttcgg caaatagctt gatgtcttcc
    35521 ttgtagtgac cataaaaatc aacggcttca tggtttggat agtatttgcc tggcaagact
    35581 tctttggtga tttcgcgcgg cacgccgtgt gcgccaccgg tcagaacgtc acaaatgctc
    35641 ggccctttgc cgcctttgtt ccagccgcct tcgacctgat gagcggcaac tgcgccgccc
    35701 cataagaaat ctttcggtaa ggtgagtttt ttcactatca taggctccag atgagtcaat
    35761 tcttacagga aatagtacca tgcagccaaa cgacatcact ttttttcaac gtttccagga
    35821 tgacattctg gctgggcgta aaaccatcac catccgcgac gagtctgaat cgcacttcaa
    35881 aacgggtgat gtgcttcgtg tcgggcgttt tgaagatgac ggttattttt gcacgattga
    35941 agtcaccgca acctcaaccg taacgctgga tacgctgacg gaaaaacatg cagagcagga
    36001 aaatatgacc ctgactgaac tgataaaggt cattgccgac atctatcccg gtcagacaca
    36061 gttttatgtg attgaattta aatgtcttta aattgaactc acgcctgtta gttgaaattt
    36121 cgcatttaat ttcatgattt ttaggagatt agtctcaaat cagaatttat tttagctaac
    36181 aggtgttcac tggaactatt ctcagttacg ctggagaggt atacatgtgc gtgtacgttt
    36241 ctccggagta agttatggtt cagaagcccc tcattaagca gggatattcg ctggcagagg
    36301 aaattgccaa cagcgtcagt cacggcattg ggttggtgtt tggtatcgtt gggctggtgt
    36361 tgctactggt tcaggcggtg gatctcaatg ccagcgccac agcgataacc agttacagcc
    36421 tctatggcgg cagtatgatc ctgctgttcc tcgcttcgac gctctatcac gccattcctc
    36481 atcaacgggc aaaaatgtgg ctgaagaaat ttgaccattg cgctatttac ctgttgattg
    36541 ccggaaccta cacgccgttt ttgctggtgg ggctggattc tccgttagcg cgcgggttga
    36601 tgattgttat ctggagcctg gcattgctgg gtattctgtt taaactgacc atcgcgcacc
    36661 gattcaaaat tttatctctg gtgacctatc tggcgatggg ctggctgtcg ctggtggtaa
    36721 tttatgaaat ggcagttaag ctcgcggcgg gcagcgttac cttactggcg gtaggcggcg
    36781 tggtttattc gctcggggtg attttctacg tctgcaaacg cattccatac aaccatgcca
    36841 tctggcacgg cttcgtgctc ggcggtagtg tgtgccactt tctggcgatc tatttgtata
    36901 ttgggcaggc gtaatgggta tgcctttgta ggcctgataa gacgcgccag cgtcgcatca
    36961 ggcgataaat gcctgatgcg ataaagagat ttactcttcg agcgaatacg gcagcggctc
    37021 gatatgcaat gtattcgcat cgtcgcgtac gcggaagatg ctatccggtt ccatatcgtt
    37081 attcatgacg acctgtacca cgacctgacc atcttccagt tttaccgcag ccagcaccgt
    37141 accggtacga cgccagttct cgcccatttt cagctctaag tcttcaccag cttccggcag
    37201 tcggctggcg ctacctgcca gcaaccagag cgcacgttta ttggcaccac ggaattttgc
    37261 tcgcgccacc atctcttgtc cggtataaca gcctttctta aagctgatac cgcccagcgc
    37321 ctggagattg gtcgcctgtg ggataaactg cccgctgttg gcggcatcaa tcaccgggaa
    37381 acccgcttca atgtttaatg ccagccactg ttggctattg ttcagttccg cttcaccgcg
    37441 cagtttatcg gtcagcatat tagcagtagc ttcatcggtt acgatcagga aacgttctgc
    37501 cgggtgttca aaccatagca aagtggtcgc gccttctttg actacctgtt tttctttcga
    37561 aggcagttcg ctaaagagat ttgccagcgc ggcgcgcgcc tgaaaaccgg caacaccaag
    37621 cagcacacgc tcgtcgtctg gcgcgatggt cactttagag aataccgcat attttttcag
    37681 ttcagtcagc tgcggttcac gcacgctgcg ccgttcaatc catgcaaagc catcgccgtc
    37741 gcggaacaga cgtaaattgc tccacatttt acctttggcg tcgcaatggg cggcgagcag
    37801 gtgctgatct tctgccatct ggctgacatc tgctgtcacc tgaccctgca tatatttttc
    37861 gctgtccgcg ccagtaatgg tggcaagcgc ccagtcatca agcgtcatca gcgtcagcgg
    37921 taaacgggca gaagccgtag gctgacgggg aggaaaaggt gtaaaagcca taatagagtc
    37981 ctgagttgct taacggcgta ttaatgacta atggtaaaag agctatgagg caatgcaagt
    38041 gctttcacta cgcgatctgt gccttctgcg aggcgacttc caaaaaaatg aagccattga
    38101 attgttgtga attaatttcg cgatcgcgat ggtgtttact gatattcccg taaaagaagg
    38161 cgaaaaacac gttacaatag ccgggtactg gattttcgtg agacaggaag aagatggaca
    38221 ttaacaacaa agcccgcatt cattgggcat gccgccgtgg tatgcgcgaa ctcgatattt
    38281 caatcatgcc gtttttcgaa catgagtacg acagcttaag cgatgacgaa aaacgcatct
    38341 ttattcgtct gctggaatgt gacgatccgg acctgtttaa ctggctgatg aatcacggta
    38401 aaccggccga tgcagaactg gaaatgatgg tccgactcat ccagacacgg aaccgggaac
    38461 gtggtcctgt ggcaatctga tttgcgcgtc tcctggcgcg cacagtggct ttccttgctg
    38521 attcatgggc tggttgccgc tgttatttta ctcatgccct ggccactcag ttacaccccg
    38581 ttatggatgg tgttactttc gctggtggtg tttgattgcg ttcgcagcca gcggcgtatt
    38641 aatgctcgcc agggggaaat tcgcttgttg atggacgggc gtttgcgttg gcaagggcag
    38701 gagtggagca tcgtcaaagc accgtggatg attaagagcg gcatgatgct gcgtttacgt
    38761 tctgatggcg gtaaacggca acatttatgg ctggcagccg acagcatgga cgaagctgaa
    38821 tggcgggatt tacggcggat tttgttgcaa caagagacgc aaagataaag atgacggcaa
    38881 agcatatgcg ctaagccgtc agtgagatca ggcgtaatgc tctgccattt cgttgaggat
    38941 ttgctcgcac cagctctgaa tacgctcgtc gctaaggtca tactggttag tttcatccag
    39001 cgccagaccc acgaacagtt gcccgtcagc aatcaccggt ttcgggctgg taaattcata
    39061 tccttccgtt ggccagtagc cgacgaactt cacgcctttg gtcgagagtt tgtcatgcag
    39121 cataccgagc gcatcgagga accactcgcc gtatcccagt tgatcgccaa gcccatacag
    39181 cgcaacaatt ttaccttcaa ggttcaggtc gtcgagctga tcccagacgg cttcccagtc
    39241 ttcctggatt tcaccaaaat cccaggtcgg gatacccaga atgagcacat cgtactgctc
    39301 cattaatttc ggggagtcgt ccttgaggtt atgtaaggtc accagttctg ggccgataat
    39361 atcgcggatt ttttctgccg ccatttcggt gtaacaggtg ctggaaccgt aaaaaagacc
    39421 catattcata acgtaagctt ctcgattctg ctgtggcttt tgcagctagt gtatcagaat
    39481 cgctttagtc acggcacaat aaggcataat ggatcaaatg agtgaccata aggggcgcaa
    39541 gtgaaacagg atctggcacg catcgagcag tttcttgatg ctctgtggct ggaaaaaaat
    39601 ctggctgaaa atacgttgaa cgcttaccgt cgcgatctgt caatgatggt ggagtggttg
    39661 catcaccgcg ggttgacgct ggcgacggcg caaagtgacg atttgcaggc attactggca
    39721 gaacggctgg agggcgggta taaagccacc agctcagcgc gtttgctgag tgcagtgcgc
    39781 cgattgttcc agtatcttta tcgcgaaaag tttcgtgaag acgatcccag tgcgcatctc
    39841 gcttcaccga aattgccaca gcgtttgcca aaagatttaa gcgaagcgca ggtcgaacgt
    39901 ttattacagg caccattaat tgatcagcca ctggagctac gcgataaagc catgcttgaa
    39961 gtgttgtatg ctaccgggct gcgtgtctct gaactggtcg gactgacaat gagtgatatc
    40021 agcctgcgtc agggcgtagt gcgggtcatt ggtaaaggca acaaagagcg cctggtgccg
    40081 ttaggtgaag aggcggttta ctggctggaa acgtatctcg agcatgggcg accgtggttg
    40141 ttgaacggtg tctctattga tgtgttgttc cccagccagc gtgcgcagca gatgacgcga
    40201 cagaccttct ggcaccgcat taaacattat gccgtgctgg cgggtatcga cagcgaaaag
    40261 ctgtcaccgc atgtgttgcg tcacgctttt gccacccatt tattaaatca tggtgcggat
    40321 ttacgcgtgg tgcagatgct actgggccac agcgacctct ccaccacgca aatttatacg
    40381 catgtcgcta ccgagcgtct gcggcaactt catcaacagc atcacccgcg gacgtgatgt
    40441 ctgaaaagaa cgggaagatt tatgaagaaa ggttttatgt tgtttacttt gttagcggcg
    40501 ttttcaggtt ttgctcagga tgatgacgcg gcaattcaac aaacgttagc caaaatgggc
    40561 atcaaaagca gcgatattca gcccgcgccc gtagctggca tgaagacagt tctgactaac
    40621 agcggcgtgt tgtacatcac cgatgatggt aaacatatca ttcaggggcc aatgtatgac
    40681 gttagcggca cggctccggt caatgtcacc aataagatgc tgttaaagca gttgaatgcg
    40741 cttgaaaaag agatgatcgt ttataaagcg ccgcaggaaa aacacgtcat caccgtgttt
    40801 actgatatta cctgtggtta ctgccacaaa ctgcatgagc aaatggcaga ctataacgcg
    40861 ctggggatca ccgtgcgtta tcttgctttc ccgcgccagg ggctggacag cgatgcagag
    40921 aaagaaatga aagctatctg gtgtgcgaaa gataaaaaca aagcgtttga tgatgtgatg
    40981 gcaggtaaaa gcgtcgcacc agccagctgc gacgtggata ttgccgacca ttacgtactt
    41041 ggcgtccagc ttggcgttag cggtactccg gcagttgtgc tgagcaatgg cacacttgtt
    41101 ccgggttacc agccgccgaa agagatgaaa gaattcctcg acgaacacca aaaaatgacc
    41161 agcggtaaat aattcgcgtg aaacaacaga tacaacttcg tcgccgtgaa gtcgatgaaa
    41221 cggcagactt gcccgctgaa ttgcctccct tgctgcgccg tttatacgcc agccggggcg
    41281 tgcgcagtgc gcaagaactg gaacgcagtg ttaaaggtat gttgccctgg cagcaactga
    41341 gcggcgtcga aaaggccgtt gagatccttt acaacgcttt tcgcgaagga acgcggatta
    41401 ttgtggtcgg cgattttgac gccgacggcg cgaccagcac ggctctaagc gtgctggcga
    41461 tgcgctcgct tggttgcagc aatatcgact atctggtacc aaaccgtttc gaagacggtt
    41521 acggcttaag cccggaagta gtcgatcagg cccatgcccg tggcgcgcag ttaattgtca
    41581 cggtggataa cggtatttcc tcccatgcgg gcgttgaaca cgctcgctcg ttgggcattc
    41641 cggttattgt taccgatcac catttgccgg gcgaaacatt acccgcagcg gaagcgatca
    41701 ttaaccctaa cttgcgcgac tgtaatttcc cgtcgaaatc actggcaggc gtgggtgtgg
    41761 cgttttatct gatgctggcg ctgcgcacct ttttgcgcga tcagggctgg tttgatgagc
    41821 gtggcatcgc aattcctaac ctggcagaac tgctggatct ggtcgcgctg ggaacagtgg
    41881 cggacgtcgt gccgctggac gctaataatc gcattctgac ctggcagggg atgagtcgca
    41941 tccgtgccgg aaagtgccgt ccagggatta aagcgctgct ggaagtggca aaccgtgatg
    42001 cacaaaaact cgccgccagc gatttaggtt ttgcgctggg gccacgtctc aatgctgccg
    42061 gacgactgga cgatatgtcc gtcggtgtgg cgctcttgct gtgcgacaac atcggcgaag
    42121 cgcgcgtgct ggcaaatgaa ctcgatgcgc taaaccagac gcgaaaagag atcgaacaag
    42181 gaatgcaagt tgaagccctg accctgtgcg agaaactgga gcgaagtcgc gacacgctac
    42241 ccggcgggct ggcaatgtat caccccgaat ggcatcaggg cgttgtcggt attctggctt
    42301 cgcgcatcaa agagcgtttt caccgtccgg ttatcgcctt tgcgccagca ggtgatggta
    42361 cgctgaaagg ttcaggtcgc tccattcagg ggctgcatat gcgtgatgca ctggagcgat
    42421 tagacacact ctaccctggc atgatactga agtttggcgg tcatgcgatg gcggcgggtt
    42481 tgtcgctgga agaggataaa ttcgaactct ttcaacaacg gtttggcgag ctggttaccg
    42541 agtggctgga cccttcgcta ttgcaaggcg aagtggtgtc agacggcccg ttaagcccgg
    42601 ccgaaatgac catggaagtg gcgcagctgc tgcgcgatgc tggcccgtgg gggcagatgt
    42661 tcccggagcc gctgtttgat ggtcatttcc gtctgctgca acagcggctg gtgggcgaac
    42721 gtcatttgaa agtcatggtc gaaccggtcg gcggcggtcc gctgctggat ggtattgctt
    42781 ttaatgtcga taccgccctc tggccggata acggcgtgcg cgaagtgcaa ctggcttaca
    42841 agctcgatat caacgagttt cgcggcaacc gcagcctgca aattatcatc gacaatatct
    42901 ggccaattta gcgtcatctt ctctataaaa aagagcgtgg attgggtaca atcccgctct
    42961 tatcaccgca ttttgactag ctcaataaaa gaaatcagac catgtttgaa attaatccgg
    43021 taaataatcg cattcaggac ctcacggaac gctccgacgt tcttaggggg tatctttgac
    43081 tacgacgcca agaaagagcg tctggaagaa gtaaacgccg agctggaaca gccggatgtc
    43141 tggaacgaac tcgaacgcgc acaggcgctg ggtaaagagc gttcctccct cgaagccgtt
    43201 gtcgacaccc tcgaccaaat gaaacagggg ctggaagatg tttctggtct gctggaactg
    43261 gctgtagaag ctgacgacga agaaaccttt aacgaagccg ttgctgaact cgacgccctg
    43321 gaagaaaaac tggcgcagct tgagttccgc cgtatgttct ctggcgaata tgacagcgcc
    43381 gactgctacc tcgatattca ggcggggtct ggcggtacgg aagcacagga ctgggcgagc
    43441 atgcttgagc gtatgtattt gcgctgggca gaatcgcgtg gtttcaaaac tgaaatcatc
    43501 gaagagtcgg aaggtgaagt ggcgggtatt aaatccgtga cgatcaaaat ctccggcgat
    43561 tacgcttacg gctggctgcg tacagaaact ggcgttcacc gcctggtgcg taagagcccg
    43621 tttgactccg gcggtcgtcg ccacacgtcg ttcagctccg cgtttgttta tccggaagtt
    43681 gatgatgata ttgatatcga aatcaacccg gcggatttgc gcattgacgt ttatcgcgcg
    43741 tccggcgcgg gcggtcagca cgttaaccgt accgaatctg cggtgcgtat tacccacatc
    43801 ccgaccggga tcgtgaccca gtgccagaac gaccgttccc agcacaagaa caaagaccag
    43861 gccatgaagc agatgaaagc gaagctttat gaactggaga tgcagaagaa aaatgctgag
    43921 aaacaggcga tggaagataa caaatctgac atcggctggg gcagccagat tcgttcttat
    43981 gtccttgatg actcccgcat taaagatctg cgcaccgggg tagaaacccg caacacgcag
    44041 gccgtgctgg acggcagcct ggatcaattt atcgaagcaa gtttgaaagc agggttatga
    44101 ggaaccaaca tgtctgaaca acacgcacag ggcgctgacg cggtagtcga tcttaacaat
    44161 gaactgaaaa cgcgtcgtga gaagctggcg aacctgcgtg agcagggaat tgccttcccg
    44221 aacgatttcc gtcgcgatca tacctctgac caattgcacg cagaattcga cggtaaagag
    44281 aacgaagaac tggaagcgct gaacatcgaa gtcgccgttg ctggccgcat gatgacccgc
    44341 cgtattatgg gtaaagcctc ttttgttacc ctgcaggacg ttggcggtcg tattcagctg
    44401 tacgttgccc gtgacgatct tccggaaggc gtttacaacg agcagttcaa aaaatgggat
    44461 ctcggcgaca tcctcggcgc gaaaggtaaa ctgttcaaaa ccaaaaccgg cgaactgtct
    44521 atccactgca ccgagctgcg tctgctgacc aaagcactgc gtccgctgcc ggacaaattc
    44581 cacggcttgc aggatcagga agcgcgctat cgtcagcgtt atctggatct catctctaac
    44641 gatgaatccc gcaacacctt taaagtgcgt tcgcagatcc tctctggcat tcgccagttc
    44701 atggtaaatc gcggctttat ggaagttgaa acgccgatga tgcaggtgat cccaggcggt
    44761 gcggctgcgc gtccgtttat cacccatcat aacgcgctgg atcttgacat gtacctgcgt
    44821 atcgcgccgg aactgtacct caagcgtctg gtggtcggtg gcttcgagcg tgtattcgaa
    44881 atcaaccgta acttccgtaa cgaaggtatt tccgtacgtc ataacccaga gttcaccatg
    44941 atggaactct atatggctta cgcagattac aaagatctga tcgagctgac cgaatcgctg
    45001 ttccgtactc tggcacagga tattctcggt aagacggaag tgacctacgg cgacgtgacg
    45061 ctggacttcg gtaaaccgtt cgaaaaactg accatgcgtg aagcgatcaa gaaatatcgc
    45121 ccggaaaccg acatggcgga tctggacaac ttcgactctg caaaagcgat tgctgaatct
    45181 atcggcatcc acgttgagaa gagctggggt ctgggccgta tcgttaccga gatcttcgaa
    45241 gaagtggcag aagcacatct gattcagccg accttcatta ctgaatatcc ggcagaagtt
    45301 tctccgctgg cgcgtcgtaa cgacgttaac ccggaaatca cagaccgctt tgagttcttc
    45361 attggtggtc gtgaaatcgg taacggcttt agcgagctga atgacgcgga agatcaggcg
    45421 cagcgcttcc tggatcaggt tgccgcgaaa gatgcaggtg acgacgaagc tatgttctac
    45481 gatgaagatt acgtcaccgc actggaacat ggcttaccgc cgacagcagg tctggggatt
    45541 ggtatcgacc gtatggtaat gctgttcacc aacagccata ccatccgcga cgttattctg
    45601 ttcccggcga tgcgtccggt gaaataagca ttacgttatg ctcataaccc cggcaaatgt
    45661 cggggttttt taattgtgct gcgcgaaagc agacaacaat tttcttgctt cactattggc
    45721 tgcctgcatc accatccacg gactgaacgc ccacggcgtg gcatcaatac cgtgtaatac
    45781 atctgctaaa tcacaccatt gataatccat cacttcatca tcattgatct gtaacgcact
    45841 ggtcgtgcgt gcggcaaata ccggacacac ttcattttcc acaatgccac tcggatcggt
    45901 agcgcggtag cgaaagtcag gatagataga ttcaggaggc gtaatttcca cgccaagctc
    45961 ataacggcaa cggcggatca ctgcgtcttc gttgctttct cccagttgtg ggtgcccaca
    46021 aaccgagtta gtccacacgc caggccatgc ttttttgctc agtgcgcggc gggtaactaa
    46081 taattgtcct ttggcattaa acagccaact ggagaacgcg agatgtaaga gggtgtctgc
    46141 cgtgtgtgcg gcatactttt ccagcgtacc cgtgggaact ccctgtgcat tcaataaaat
    46201 gacgtgttcc gtttgcataa tttctcacat gtaattctga tcactcatca ttatagataa
    46261 ttgaagagtg aaatgattaa ccaagtgagc gtttatcgac aaccgcccgt tttgagcgga
    46321 tgccgacagg taaaaaccat ttaaaaaagt agcatcatat atttgaagcg actttcaatg
    46381 gccattgcaa cacagtcaga aatgacagga atagagagag taaaaagcaa caacagactg
    46441 aatatagcgc gcattaaaag attcatatta ttctccatgc tcatttttct tcagcttgcg
    46501 catcagaagc cacatacgta ctgtcctgac ttttaaatct ttatcagaga cggattcctt
    46561 caccaggtca gcatgatgct gatagccatt aaagaagacg ttcagcacaa cggcgctaag
    46621 ggttgcgagc ataatgccgc tatgtagcag cggttgcagt acggccggta acttagaaaa
    46681 gaaatcgtga gagagcgtcg gagtcatgcc aacgccgaga ctgatcgcca caatatagag
    46741 gttgtaacgg ttggtggtgt agttacagcg cgacagaatt cgaatccctg tcgccagtac
    46801 catgccgaac atcaccagcc cagcgccgcc cagcacaaat tgcggaatgg aggcgaccag
    46861 caccgccatt tttggcacca tgccaaacag gattaatata attcccgaag aaatacacac
    46921 ccagcggcta tgaacacgcg tcacgctaac caggccaacg ttttgtgaaa atgacgtgtg
    46981 ggggaagctg ttaaacgttc cgcctatcat tgtccctacg ccatcgacac gcagcccgcg
    47041 aataatatcg tgtgaagaga gtttacgacc gactatttca cccagtgcca ggaacatccc
    47101 catcgactcg ataaacacga tgattaacac ggcagtcatg gtcagaatgg aaacgggatc
    47161 gaaaatcggc ataccaaacg acatcggggt gacaatcgca aaccacgagg catcatgtag
    47221 cccggataaa ttgacttcat tcatcatcca cgaaagtaaa aagccaaata caatccccag
    47281 taatacggcg acgttggaca taaatccttt cgcatagcga gtaatgagca agataaaaat
    47341 taagacggca aaggagatac ctaaataaac gggattacca tattgcggat tccctttacc
    47401 tccggcggcc cagtcaatac ccacctgaat gatgctaagc ccaatagaag taataaccac
    47461 accggtaacc agtggcggga ataaaggcat caagcgaccg ataagtggcg ctaataatgt
    47521 ggtgataaaa cccgcggcaa tagtggcacc aaatatcccc agcaggccga tatccgggtt
    47581 catcccaatg gctatcattg gtgttacagc agcaaaggtc accgacataa tcaccggcag
    47641 gcggatcccc ataaagcggc cgataccgat acattgcaat aatgtgacga tcccgcagca
    47701 aaagagatcc gagctaatga gcatcgcaat agcttctttt gagaggccca gtaggtcacc
    47761 aatcataaga ggaacagcga ctgcacctgc gtacatgaca aggacgtgtt gcagaccgag
    47821 tatgatcagc tttcctggtg ataatatgcg gtcaacctca tcagttgggc ggtcttgccc
    47881 agaagatgag ggaagttggg aatctatggc gctcatcagt gtttctcctt tcgcccgcca
    47941 gagtaaaaag caaccgttta cgttaatgaa actgtctaaa cacatgctcg tggagtgcca
    48001 gtttttgcga ggaagggaag ttagagacag gaattgcgga ggcaatcaca taatcgtgct
    48061 taaaccacga aaatgacaag agaacctgac aatggaatta tctgtaattt tctcatattg
    48121 atatttcatt aatcattctg gcatgtcgca ctctcgcatt taatcgtttt tatctggata
    48181 gcgctctttt gatcggcaat gttgaaataa tgaaagcggc atagtttgag gggcaataat
    48241 ttgaactcag tcgcaaaatt ataattaaaa cggtcatgct acctaatata tgcccgttta
    48301 ttgcatcgcg aaatttgaga agacataact cattaaatga aagggatgca aatgaataag
    48361 tttatcgctg ctgaagctgc ggaatgtata ggctgtcatg cttgtgaaat tgcctgtgcg
    48421 gtggcacaca atcaagaaaa ctggccgctg agtcacagtg actttcgacc gcgtatccac
    48481 gttgtaggga aaggccaggc tgcgaatccg gtggcctgcc atcactgcaa caatgcccct
    48541 tgcgttacgg cttgtccggt taatgctctg actttccagt ccgatagcgt acaactggac
    48601 gagcaaaaat gtattggttg taaaagatgc gcaatcgctt gcccctttgg cgtcgttgag
    48661 atggtcgata cgattgcgca gaaatgcgac ctttgtaacc agcgcagttc cggcacgcaa
    48721 gcctgtattg aagtctgccc aacacaggcg ttacgactga tggacgataa agggttacag
    48781 cagataaagg tggcccgcca gcgcaaaacg gcagcaggaa aagcgtcatc agacgctcag
    48841 ccatctcgca gtgcagcgtt gctccccgtt aactcgcgta aaggcgcaga taaaatttca
    48901 gcgagtgaac ggaaaaccca ctttggcgaa atatattgcg ggctggatcc acaacaagcg
    48961 acttatgaga gtgaccgctg tgtttattgt gccgaaaaag ctaactgcaa ctggcattgc
    49021 ccgctgcata acgctattcc ggattacatc cgtctggtac aggaaggaaa gattattgaa
    49081 gcggcagaac tttgccacca gaccagttcc ttaccagaaa tctgcggcag ggtatgtcca
    49141 caggaccgtc tttgtgaagg cgcatgtact ttgaaagatc actctggcgc agtcactatc
    49201 ggtaatctgg aacgctacat caccgatacc gcgctggcga tgggctggtg tcccgatgtc
    49261 agcaaagttg ttccccgtag cgaaaaagtg gcggtgattg gcgctgggcc agcagggtta
    49321 gggtgtgctg atattctggc gcgcgcagga gttcaggttg atgtctttga tcgccatcca
    49381 gaaattggcg gtatgctgac ttttggcata cctcctttca aactcgataa aacggtatta
    49441 agccagcggc gagagatatt caccgcaatg ggaatcgact ttcatcttaa ctgtgaaatt
    49501 ggccgcgata ttacctttag cgatttaact tctgaatatg atgcagtttt catcggcgtg
    49561 gggacttacg ggatgatgcg agctgatctg ccgcatgaag atgcgcccgg tgtcattcag
    49621 gctctaccgt tcttgactgc ccatacccgc cagctcatgg ggttgccgga gtctgaagag
    49681 tatccgctga cggacgtgga aggtaagcga gtcgtggtat tgggcggtgg cgatacgaca
    49741 atggattgtt tgcggacttc catccgcctc aatgccgcca gcgtgacctg cgcgtatcgt
    49801 cgtgatgaag tcagtatgcc gggctcgcgc aaagaggtgg tcaatgcgcg cgaggaaggt
    49861 gtcgagtttc aattcaatgt tcagccgcaa tatatcgctt gtgacgaaga tgggcgttta
    49921 actgcggtgg gcctgattcg taccgccatg ggtgagccgg ggccggatgg tcgccgtcgt
    49981 cctcgtccgg ttgcgggttc agagtttgaa ttgcccgcag atgttctcat tatggccttt
    50041 ggtttccagg cgcatgccat gccgtggttg cagggcagcg gaattaaact cgataaatgg
    50101 ggcctgattc aaaccggcga cgttgggtat ttacctaccc agacgcatct gaaaaaagtc
    50161 tttgctggtg gggatgcagt tcatggcgcg gatctggttg tcactgccat ggccgcagga
    50221 aggcaggcgg cgcgcgatat gttaactctg tttgatacga aggcatcgtg atgaaatcgt
    50281 taattatcgt taatccggct gactgtattg gctgccgtac ttgtgaagtg gcctgtgtgg
    50341 tcgctcaccc ttcagaacag gagttgaatg ccgatgtctt tttaccccgg ctgaaggtac
    50401 agcgactgga cagcatcagt gcgccggtga tgtgccatca gtgtgaaaac gccccttgtg
    50461 ttggcgcttg ccccgtgggg gcgctgacga tgggcgagca ggtggtgcag gccaattctg
    50521 cccgttgtat tggctgtcag agctgcgtca gtgcgtgccc gtttgggatg atcaccattc
    50581 agtcattgcc gggggatacc cggcaacaaa tcgtgaaatg cgatctctgt gaacagcggg
    50641 aagaggggcc agcctgcgtt gaatcttgcc caacacaggc gctgcagttg cttactgaaa
    50701 gagaactcag gcgagtccgc cagcagcgta ttgttgccag cggtgagaat cctctctgaa
    50761 agaaatctcc gcgctgaaag gcgcggagaa tctattagat tgcccaacca cccgcgtaaa
    50821 atgcgaccag tgcggcagta atgatcaccg taccaatatt cagcttttgc cattcgcgtg
    50881 caaagacgcg gcctacgacc agtgtcacaa agcccagcat aataccggta acgatattac
    50941 aagtcagaac gatgaacacg gcacacacca ggccagccat agcgtcaata aaatcattga
    51001 aatccagctt cgagacgtta cttaacatca gcaaacctac gtacatcaga gcgggtgcag
    51061 tggcgtaacc agggatcaaa aatgacagcg gtgataagaa cagaatcagc aggaataacg
    51121 cccccactac ggttgcggtt aaacctgttt tacccccggc ggcggttcct gccgctgatt
    51181 cgatataaac cgctgcgggc gctgcgccca ccaggccgga gaatattgaa cttactgagt
    51241 cactggtcag ggctttgccg ccgttgatga tctggttgtc tttatccaac aaattcgcct
    51301 gaccggcgac ggcacggatg gtgccagtag cgtcgaacac tgcggtcatc accaatgcca
    51361 gtacactcgg aagtacagtt ggctggagtg cacccataat atcgaggctg aaaatcagag
    51421 acttaccatc ttcgccagtc aggcttggca tcgccaccag accgtggtat ttcaccgctg
    51481 ggtcaaagat taagccgatg atcgaaattg caataatcac caacaagatc ccgccgggta
    51541 cgcgacactt ctccaggccg aagatgaccg ccagccccag caagctcatc atcaccggga
    51601 aggaggtaaa cgcaccgagc gcaactggca agccttcaat cgggtttttg ataaccatac
    51661 ccacaccgtt agcagcaatc agcagcagaa acagcccgat accgatacct gtaccgtgag
    51721 cgatacccat cggcaaatta cgtaagatcc aggtacgcac accggttacg gaaatggcgg
    51781 tgaagatgac gcccatcaga aataccgcgc ccagtgcgac aggaacgcta atttgttgcc
    51841 cgagtaccag actgaatgcg gtaaacgccg tcaaggaaat cgcgcaacca atcgccattg
    51901 gcaaattagc ccataatccc atcagcaacg agccgaagcc cgcgaccaga caggtggcaa
    51961 caaacacagc tgcgggagga aaacctgctt tgcccagcat tcccggaacg acgataacgg
    52021 aataaaccat ggccagaaag gtcgttaagc cagccagtac ttcctgacga acggtactgc
    52081 cacgagcggt aattttaaaa taattatcca gcgcgccctg tggctttggt gcgtccggtg
    52141 tttgtaggat gtctccagac ataatgatgt cctcacgagg atgttaaagt tttattatta
    52201 gttgcgttcg tacaccagac gaccatcaac gtaggtgcgg tagatcgaac ggtcatcgcc
    52261 caacgtcatc atcacgaaca atttgtcgac taaagaaaca gagttgtcat agcgcagctg
    52321 ctgtagcgga gtggcggtgg gttccatcac cacgaaatca gcctctttgc caggtaaaaa
    52381 gttgccaatc aaatcgtcaa ggcccagaga tttcgctccg ccgagcgtgg ccaggtaaaa
    52441 cgcttcatat gccgagaggc gatagccttg taattgcaat actttgtagg cttcgttcag
    52501 cgtttgcagc atgttgaaag tggttccggc accgatatcc gttcccatgc ccactttaac
    52561 tttcttctgc catgcttttt tcaagttgaa taagccgctg ccgaggtaaa ggttggaggt
    52621 cggacagaaa gcaatgctgg atttggtttc gctgagacga tcccactctt tttcttcgag
    52681 atggacgcag tgagcaaaga cacagttttt accggtcagg ccgtactgat ggtaaacatc
    52741 cagataacca tcatggtcag gataaagcga tttcacccag gcaatttcat ctttgttttc
    52801 acagagatgg gtatgtaccc acgtatccgg atattcttct ttcaggcgtt gcgccatcgc
    52861 catctgttca ggagatgagg tcggggcgaa gcgtggcgta atcgcatata gcagacgacc
    52921 atttttgtgc cagcgttcaa tcagttcttt gctttggtga tagctgcttt cggcagtgtc
    52981 gagcagataa tccggtgcgt tacggtccat catcacctta ccggcaatca tacgcatatt
    53041 gatatgactg gcggcttcaa acagcgcatc aacggattgc ggatgaacag tgccaaacac
    53101 cagcgccgtg gtggttccgt tacgtaaaag ctgcttgatg aagaacgccg acatttcgcg
    53161 ggcgtactct aaatcctcat aacgccgttc agtagggaag gtgtgtttat tcaaccactc
    53221 cagcaattgc tcaccatagg cccccaccat ttcactttgc ggataatgga tatgtgtatc
    53281 gacaaagccc ggtactatca gtttgccgcg atagtcgcgc acgcgaatgg tgtcaggaat
    53341 ttgatgcttt ccgttttccc attcgccaaa ccattccact tttccctgtt taatgagtaa
    53401 taaaccatcc tcaataaacc gcagcgcaga ggcaatctct tccgggttat cgaccgtacg
    53461 ggtgacatca ataaaactgc ctcgtaccgc ttttaacgtg tgttctcctg acatcattga
    53521 ctcctttact gttaatccat ctcttccgct gaggtaatac caggcagaac gttttcctgt
    53581 cggtagccac cagggaggat aatatttaat aaaatcgcag ttaacccgcc agcacaaata
    53641 gggttttcaa ctaatacata aatagaggct ggcaatattt taaaaatttc gggatcgtag
    53701 gagacgccaa gccctaaacc taaagaagtg gcgacaataa gtgtttcacg ccgctttaat
    53761 ccgttggtga tgatgatgcg aatcccagcg atggcaatca tggaaaacat caacgtcatt
    53821 gcgcctccca gaactgccga gggaatggtc gtgaagaagc cgccaatcat cggaaataag
    53881 ccgaggataa ccagcattac cgcgatggtt cgcccgacat aacgtgaagc gacgccagtc
    53941 atctgaataa ccccattatt ttgcgcaaac gtggttaatg gtaatgaacc gacagcggag
    54001 gcgataacag aaaccagacc atctgccagc acgccgcctt tcagtcggga ctgatactct
    54061 tccccctgaa tggggcggcg ggaaaccatt gccgtggcgg tgatatcgcc gactgcttcc
    54121 agcacgctaa gcagataaat cgtgccaacc accaggaact gatggaagct aaaactaaag
    54181 ccgtatttga acggatgcgg gatggtgatt aacggcaaat tgcgcatact gctgaaatcc
    54241 accatgccca ggcataacga tgcgatatag ccgacacata gcccaatggc gatccctccc
    54301 atgcgtagca acggactgcg acagcagtta aagccgatca ccacaattaa aaccaataaa
    54361 ccaacgccga gatgttcgta attgccgaac gtaccgctgc ttttggctgc aaatcctcca
    54421 ccaaaatcga taatgccgac tttaatcagg cttaagccga tcatcagtac cacaataccg
    54481 ctgacggtag gcgtaataac ccgacgtaaa tagggcaaga taaatgaaga tccgacaacc
    54541 agaaatgcgc caacgaagga gacgccgaga agcgacgaca tgattaactc ttcgtgaaaa
    54601 ccgtcgcttt tcatgctgct gcccagcgca atcatgaccg taacaaatga aaaattgact
    54661 gactgaattg agagtaggcc agaaccgacg atgccgtagc ggtttacttg taaccaggta
    54721 ccaataccag aggcgatcat cgccatagaa acaagatagg cagttgtttc agcggaaagc
    54781 tgtaaggccg cacccacgat taacgcgggg gttaccatcg gaacgaaaat tgccaacaga
    54841 tgggtaatgg caccaacgag agcctgatga aagggagggc gatcctccag ttcaaatata
    54901 aggtcagaac ctgcatggtt tatatcagac atcccttcac tccttttaaa gtaaatttga
    54961 tcatctgaat tagtaaaata aattcccacg aatttggggc gcaggacgcc ttgagaacgg
    55021 cttatctcat tttcgcagtc actatttagt catcgttcaa acagagaaac aggccgccga
    55081 agcggccatt tattatttat attttttcca gcgcagtgag tattttctcc ggtgtgaaat
    55141 gccattcgcg taaccagatg ccgcatgcat cgtgaattgc ggtagcaatc gccggagctg
    55201 cgccatttac accgatttcc gagatcgatt tcgccccgaa cgggccgact ttatcgtcgc
    55261 tcggcaccag cacagcgcgg aaatcacgcg gaatgtcacc aattttcggt gcgccgtaac
    55321 tgcgtaaatc acgcgttaac gggtgacctt cggcgtcata aatgatctct tcgctcatac
    55381 tgtggccgat agcacgcagg gtggcaccgt agatttgtcc caacgctaac tctggattga
    55441 ccggtgtacc gcagtccagc aaggcgtaga atttatccag gcggatttca cccgtacgcg
    55501 tgttgacggc aacttcagcg aagtttgcgc catacgggaa ggcgaaatca ggcgtgatat
    55561 aactgccagt tcccaccagt gaaccaaagc cggtgccggt ttcgccttta tgggcaatat
    55621 ccccgaaact aacttcgcct ttcttgccgc gcacgacgcc cggcgttgcc agttgaacat
    55681 ctgccactgg ctcacccaac atttgcgcgc cgtggaacag aattttctcc cgtagatttt
    55741 ccgctgccaa acgcgccgcg ttacccgaga agcaagtacc ggacgaggca tatgcgcctt
    55801 tatcaaacaa cgcatgatcg gtatcaccgg agataacatg cacgtcctgc ggtgggcagt
    55861 gcagcacttc tgctgccagt ttcgtcacta cagtatccag accagtacca atatccgcac
    55921 cgccagaatg aacgataaag gtgccgtccg attccagttt gatcatgcag ttagcctgat
    55981 cgatatccgg gatacccgat ttctgcatga taatggcgac gccgcgaccg atgtgccagt
    56041 caccattttg cggtttcggt gacgaccatt ggatcatctc gcgaccctga cgcaggatct
    56101 cttccagtgc acagctggcg gcggaaggaa cggaggtcgg cgctttacct tcaccgattg
    56161 caccgagaat tttcagctct tgcccttcgt gtacccggtt acgttcgata atttccagtt
    56221 ggtcgatctg taactgttca gccagttccg ctaatgccat ggtgatagcg aagttacctt
    56281 tcggtgcgcc ataaccctga taagcaccat ttgggcaaat gttgctgtag taggtggtga
    56341 cctggaaatc gacgttatcg cacggatata acggcagcga cagcgccggt ccgttacacg
    56401 gtacggtgag tgagtggttg ccgtaagggc cagtgttggc gcggaaatcc atcttcactg
    56461 ccgtcaggcg accatctttt ttcgctccca gtttgacggt gactttcgcg acgtgacgag
    56521 aggtgttagc aataaactct tcttcacggg tgtagcggaa cagtaccgga cgcccggtca
    56581 cgcaggttgc ccaggcgcac acttcttcca gcaggatgtc ctgtttggaa ccaaaaccgc
    56641 cgccaactcg ctctttaatg acatgaactt tatgctgttt catgcccacg aggcgcgcga
    56701 cctggcggcg taagtgccat ggtacctggg tggaggcgtg gataaccaga cgatcgccgt
    56761 ccatacgagt aaagcagata tgtgtttcag tcgggcactg ctgcgcttga gttgagttat
    56821 aggttcgctc aatgatcaca tcggcatcgg caaagccttt gtccatatcg ccaatatgac
    56881 catgaatact ggcggcgata tttttgcgag ggcgagaacc gatcgggaag ttgatgatca
    56941 tatgctcgcc gcgctgggct gcatggctgt tatcgtcttc cagagtatct ggcgcaccag
    57001 caacatacac caccggttca tcgtgcacga caggcgcatc ttccgccatt gcttcgtcga
    57061 tcgacattac cggcttaagc acttcatatt caacgtcgat gagcttcagt gcttcgagcg
    57121 caatttcttc actttctgcg acgaccgcag caacacgatc gccgacgtga cgcattttct
    57181 tgccgaacat acggcggtca agcggtgacg gttccggtgc gctctgacca cccggggtat
    57241 agtagatatc cgggcaattc aggtgagtaa taacgtgaac gacgcccggt aaggcttcag
    57301 ctttgctgac atccagatga gtaatcagtg cgtgagcgtg tgggctacgt aacattttaa
    57361 tgacgcaggc gtcagccgtt acgcggtctt caacatagca gggtttcgcc tgcaccattt
    57421 tcgcggcatc agttttagga taatgcttac caatgacttc taggtcgtca cggaaagtca
    57481 gagcgatatc aatggtggcc tgcggattat ttttacgtgc aaccgccagt tcaatgacct
    57541 gatagtattg ctgccagcca gcatcgcggc tgaacaaacc agaaagcgcg tcgtcgatct
    57601 cttcgcgggt aggtgcggcg atgcgatcga gaagatcggt gataatcaga gctgcagctg
    57661 gatcgttata accagactgc accacgccaa catcaaccat tgcctgttga accagactta
    57721 actcgttcca tttgcccaga gattctgcgg tacgaatatt tgccttctct aactgtgcgg
    57781 caataagcaa ggacgcgtta acgatattac cgttaaagat tattgcgtca gaaccggcaa
    57841 acccgaaacc atcatcactg ttgcgtacag agtgcattcc catgttaaac aacagctttt
    57901 gcacgttttc gcctggatta acggttagct cctgaggcgc gccatttaaa gtaaagtgga
    57961 tgatcatacg gcttcctccc cagcctgttg gcagtcggca tacaggtcgg ctaccagaac
    58021 tcccgtgata tagcgtttat aggccacgct gccccgcaaa tcttcctgcg ggaaaatggc
    58081 gttggcgaca gcctgttcaa gtgcattgcc ttccagattt tgtttttcga catcgtgcag
    58141 acgcagtgct ttactggcga cgccatccag cgcaattcgc ataccgtcgt ggtctgtcat
    58201 tgcaacggct gccgtcacga cggttaaacc tgcctgagaa cggctaattt tgcgggtcgc
    58261 acaggtgcga tacggatctt taatgataat ttcggttagc aggcgatcgc atgggcaggc
    58321 caggtagtcc tcgattgaca gcgtttcgcc gttgccaaaa accagttcag catccagtgc
    58381 caacaggacg ggaagcagca ccgactcttc ctggcgggcg gcgatttcac caccaatggt
    58441 cgactgatta cgaacatggc gtgagtaaac aaaaccgaga gcttcacgca gcgctgcagg
    58501 aataaatcgc gcatcacgca gtggctgcaa gcgagacatt gcgccaatcc gcagtgcacc
    58561 gttatcccag tcaacccagt ccagttccag atcctgtaag gaaatggcaa tctttttatc
    58621 ggtacgggtt ggtgtagcat tgagtttgct gcccccggcg aaccagacgg cttcatcctg
    58681 gtagcggcgc ttcagttcca gcgcctgttc gacagagtcg ggcctgaaaa attgttcaat
    58741 catcttcttt ccttatttaa ccctcttccc tgcgggaaga gggcatctgt catttatgcc
    58801 agcgcatcca tccgacgcca catactggca gcggcttttc tggcttgtgc ataaatggaa
    58861 tcgcaatcga agttaaactg acggtcttca tagaccatca caccattgac catcacgctg
    58921 tgaacgctgc ctgagcccat accgaaagcg atatgcccgg cgatattgtc tgccagcagc
    58981 ggcgtcggcg agttgtaatc gcaaatggtt aaatcagctt tgtaaccggc ttccagaagc
    59041 ccaaatttcg cgccaaagtt gcggctcatc agttcgttac cgttagtcag ggctttggca
    59101 aaactgtcag gccacagcgg accacccgca tcgcgatgtt taaagaaggc aaatttcatc
    59161 tcttcaaaca tgtccgaacc aatgccgtcc gttcccaacg ccagattgcg gatgtcgcta
    59221 aggtgatggt tgtagccgac atggttgttc atgtttgaac gggcgttatg caccaggaac
    59281 gcatcgcgct gattgagtag ggtgatgtca tctttcgaca agtacagccc atgagcgacc
    59341 agcgttttgc tgtcgatgag atcgaattgc gccagtcgtg ccagcaggtc tttgccgtac
    59401 cagtggtgac tgtaggaaac gtcgtaaagg tcttccgcag cgtgaatatg caaaccacgg
    59461 cctgtggctt tcacggcttc acgcagcatc tccagaccgg catccggcac ggtaaacggc
    59521 gcgtgagcac cgatatgtgc ttccaccaga tacggctcgc ttgtcgcttt cttcgcctca
    59581 tcaatcaaac gggcgaaacg gatgttttct tctacacctt cctgcaactc tttgatgccg
    59641 ttgttacggt cagtagtttc aaaacaggtc atcgcgcgca ggccaacttt taaaaatgcg
    59701 tcgcgcaatg tggagagcga cccgccgata tacgccggag aggcatggtg atcgataacc
    59761 gatgtacatc cgctcttaat cgcttccagg gaacaaatca gtccgctgta atagagcgac
    59821 tcttcatcaa gggcgcgatc gagccgccac cagagatttt tcagcgttga gatgaaatcc
    59881 gggcaagggg cgatgtttgc cataattccg cgggaaagcc ccgagtaaaa atggttgtgc
    59941 gagcagacaa tccctggcat cacaatccgg ccatgcatct ctttgaagct ggcgtcgggg
    60001 tagcgttgcg tcagggcatc gccgatagcg acaatcacat cgttttcgat ggcgatatca
    60061 acgccttcct gcacttttgc cgggtgtagc tgtaccgcag tgacattctt cagaatcaac
    60121 atgattacac ctccacgcgg cccagcagat aatgatgatg ctggtggaca tggctgatga
    60181 tgcggcacat atcgttcagc tccggtggta cgttgttaaa ctgacctttg ctgtcgatgt
    60241 ttaacaccca gctttggtta ttcagacgta ctcgtacccg gcaatcttcc acaaggaagc
    60301 ctgggttgct gctgttatca aagtcttgcg ccaggttgaa gacggtgatt ttgtctttgt
    60361 acggtttacc gttccacgga cagaactgag cgcagttgcc gcattcgtta cagtaagcgt
    60421 cgaggtgcag cgtctggaaa cggttctgga agcctgggac cgcaatggag acgttggcgc
    60481 ggttcgggca gacatccaca cacttgctgc aaacgtagtt acattcgagg cagcgagcgg
    60541 cttcctgggc gacaaacgcg tcacgatcgt cactgttcac cagcgtgata gagatatcgc
    60601 ctttacgttg atagatttcc gctggattga cgttgttcca gtatttatcg ttctggtggg
    60661 aacggatatt ttcccgacta aggatggcat cggtcgcccg acgcgcggtt ccgacagcag
    60721 cgacaatgga ggatggtccg cgctgtacgt cgccgatcat aaagacgtca gtcagacgag
    60781 tttcgccatt atggtcgacg tctggccagc cgtttttgtc cagcggcacg cccatcgcat
    60841 tcagggcttc agtatcctgc tgttcaccaa tggcggtgat caggctgtct acaagcagtg
    60901 ttactgtttc attggtttca accggacgac gacgaccttt ctcatccggt tcgccaagcg
    60961 acataacgcg caaggttaag gtgccatcag catcgaaacg ttccggatta ttcaggaaac
    61021 ggaactctac gccgtcgtgc aacgcttctt catactcttc gcgccatgcg ggcatctctt
    61081 gtagtgaacg acggtaaacg atcgttgctt tttctacgcc tggaacgcgt aacgccgcac
    61141 gagcgcagtc cattgcggtg ttacccgccc cgacaacgac cacatgtttg cccagcttga
    61201 gcgctgtacc cttgttgtat tcacgcagga aggggagtga tttccagaca ttttggttgt
    61261 cgcccgccag tttcacaccg ctatttttat cagtgccggt ggcaatcaga acatagtgga
    61321 agccctgatt ttttaactgc tcaatggtta aatcgggtga gcagccatac tcaaatttca
    61381 cgccgtgagc ggcaacaaaa tcgatatcgt gctgaattaa ctctgcagga atacggaact
    61441 gaggaatgat atttttcacc acgccgcccg cattggcttc gcgttcaaac agcgtaaccg
    61501 gatggcccgc tctggcaagg aagtaacctg ctgccagacc cgccggacct gcaccaatca
    61561 cggcaaccgg atggcgtgaa ccagaaccgg ctggtttgtg ccagcgttgc ttatattcat
    61621 cccaaccttt ttccagcgcg acttttttca gttcgcggat attcagcgca ctgtcgtaat
    61681 ccaggcgggt acagttgtat tggcactggt gatcgcaaat atgaccggta atggcgggca
    61741 gggcgttgcg ttggtagata agttccagcg cgtcggcata gcggtgttcg ccaagcagac
    61801 ggatgtattc cggaatatct tgcttgatag cgcaggcagt aacacagggg gcaacgtagc
    61861 agtcggtcag cggcaggtct tctgccactt caatacgctc ttctggcttc cagtgtttct
    61921 gggtgtattc catggttagc gcgtctgctg ccagtctgtt cagtcgttcg acgtcaacat
    61981 ggtcaagtcc ccaggcgtcg gagccttcca gctcgcgcat gcaggcactt aagcgcagat
    62041 agccaccagg tttcagcagg tcggttgcca tcgtgatagg gcgaatacca gtatcaaaaa
    62101 tatcgcggat agtcagctga ctggcaccac cggaataaga aatgggcagt ttgccgtcaa
    62161 aggcgcgtga gagaactgct gcaacattga tggagagcgg gaacagcgca cggcctgaca
    62221 tatacatctc ttcaccgggc agtgcgcctt tattgttgat ggtgccgaga gtgttagtca
    62281 gttttacgcc aaagccgagt gatttttctt ttgccagtgc catcaggcgt tccagcattt
    62341 ccagcgcttg cgtcagcttg aggtcgtgat caaatgactc ttcttttaag cctatgtaac
    62401 cgaaaccgca gacatcgagg atctcacgaa cacgcgcgta ccccagtaag gttgggttaa
    62461 gtttgacaaa ggtgttgagc cctttttctt ccagcatgta acggcaaatg gcttcgattt
    62521 catgcggagg acagccgtgc atggtggaga gggtgacgcc ctgcaccata ccggtgggga
    62581 tgcgagcggg taaagcttgc aagctttcgc gtttttcctg caatccgtgg cgggctaaaa
    62641 atgcgtcatc ctggagtaat ttattcagcg tatcgcgata ttgcgcgaat ttcggatggt
    62701 cagatgcgtc catcatattg tcgatgaact gttgcatcgg cggttgctta ataccttcaa
    62761 ggttgtaacc gacgctcata ttaaagatga acgatttacc ggaatcagaa ggctggaaca
    62821 tcgcttcgag aaggtgcagg gcaaaccagg ctttgaggta ttcatcccag gctttaagca
    62881 gggtaaattc ggtagaccat tcggtgttaa agcactcgtc ttcggcatcg atacaaggct
    62941 tttccagctc caggcggtca agaatttgga cggtttttag ttcgatgaag cgtccgccag
    63001 tcagccagga agtgacaata ttttgcgcga gctgcgtgtg cgggccagcg gcagggccga
    63061 cgggagtggc acaggtttca ccgaagacgc taacagtttt accttttacg ggtgagtaaa
    63121 actgttgctc gggaatacca aagattgagc gttgttgttg gtattcatca aatatgcgcg
    63181 tcaaaagttc ctcaaacgga atgggacgca taatatcccc cataaccctc tccttagctt
    63241 atacagatat cattgagtga ccggtgttga gaaatccggt ggtttagggg gatagagcaa
    63301 caatcgcacc actcttgcca ggtttggcgg gaaatataaa aaaatgtgag gcggctcgaa
    63361 acttaagggc tttttgtgaa tgaactcaca ggtgccaaat gagaattaaa atcactcctg
    63421 aaaattatta ttgccagccc atttatcact ttttctcttt tctcaaaatc agtgattcct
    63481 tatcattatg ataaagagtg ctggaggggt taaatatttg aacgcaatcg tttaactaca
    63541 ttttctaaat ttcagtatac cttttcttcg ctgtaataaa gtcgtccgga gtatcaatat
    63601 ctaaaattat ttctgcattt tctatttcga cggaataatg atctcccata aggagtgcct
    63661 gacgcatatt ggtgacattg ggtcgctgga atgcctgcat caagcatggt tttgacacta
    63721 aaatcggatg gccggggatg ccattatgga gtggcagtat tgcgccatca tttcgtaacg
    63781 accagatttt tctaaaaata tcaatggtga gggttggcat gtcaccgtgg gtgagaaaac
    63841 aatgttctgt ttgcactgcg ggtactgcgg ccttgacgga tgttagtaaa ccctgggcat
    63901 aatctgggtt gtgaataata gtgatattac tctggttcgc ataacgttcg tgcagttcat
    63961 taccacgata gccggtgact aaaataattc ggctacaaaa ctgcaacgca tttttgatac
    64021 ttgtatcaag aattgttccc tgttgccagg gtaacatcat tttccattgc cccattcttg
    64081 atgataatcc ggcggcggta attatacagt cgatggctga catagtggtt ccattattta
    64141 taggggctaa taacgcctga taattgcgtt acttcctgtt ggattaagtt gggggtaatt
    64201 tatcccagag aggtcataaa gactcgtgaa aagtatagtt gacccatcgg cattattcat
    64261 tgatttaggt gcgcagaaac gtcccactgt gatttctgtt gttggtgctg gggggaaaac
    64321 cagcttgctt ttttggctgg cagaactgct ccagactcgc ggcaggcgtg tgttaatcac
    64381 tacgacgaca catatgttta tgccaaagtc acactggccc gtggttttct gtcgtgatcc
    64441 cgccatgctt cttcatgcgt ctcttacatc ccccatttca ttttgttttc acagctggaa
    64501 agcgaaccag ggaaaagtgc agggatttac gccagaagcg attgatgcac tggtgcaacg
    64561 accagagtgt gacgtaattc tcattgaagc agatggctcg cgtggaatgc cgttaaaagc
    64621 gcctgatgag cacgaacctt gcatacccaa aagcagttgc tgcgtgattg ctgtgatggg
    64681 aggacatact ttgggcgcga aagtgagcac agaaaatgtc catcgctggt cgcagtttgc
    64741 tgatattacc gggttaacac ctgatgcaac cttgcaactg agcgatctcg ttgcgctggt
    64801 tcgccaccct cagggggcgt ttaaaaacgt accgcaaggt tgtcggcgag tctggttcat
    64861 taaccgtttt tctcaatgtg agaatgcgat tgcacaaagc gagctccttc aaccgctgca
    64921 acaacatgac gtagaggcaa tctggctggg cgatatacaa gaacatcctg caatcgcgcg
    64981 cagatttgtg aattagcggc ccgggtattc cgggttactg aaaggatatc cattgaggtt
    65041 ggtatgaata ttttcacaga ggctgcaaaa ctcgaagagc aaaattgtcc gtttgcgatg
    65101 gcgcaaattg ttgatagccg aggctcgact ccccgccatt ctgcacaaat gttagtgcgc
    65161 gccgatggtt ctatcgtcgg tacaattggt ggcggaatgg ttgagcggaa ggtgattgaa
    65221 gagtcgcttc aggcattgca ggaacgtaag ccgcgattat tccatggacg tatggctcgt
    65281 aacggtgcgg atgctgtcgg gtcagattgt ggaggtgcaa tgtcagtatt tatcagcgtc
    65341 catggtatgc gtccacgtct ggtgttgatt ggcgcggggc atgtcaaccg ggcgatagcc
    65401 cagagtgccg cgctattagg atttgatatt gccgttgccg atatttatcg cgaaagcctc
    65461 aatcctgaac tattcccacc atcaaccacg cttctccatg ctgagtcgtt tggtgcggca
    65521 gtggaagcac tggatattcg ccctgataat tttgtcctga ttgccacgaa taatcaggat
    65581 cgtgaagccc tcgacaaact cattgaacag cccattgcat ggttggggtt gctggcaagt
    65641 cgccgcaagg ttcagctttt cctgcgtcaa ttgcgtgaga aaggcgtggc tgaagaacat
    65701 attgcccgtt tacatgcgcc cgttggttac aacataggtg cggaaacgcc gcaggagatc
    65761 gccatcagcg tgctggcaga aatattacag gtgaaaaata acgcgccggg tgggctgatg
    65821 atgaaacctt ctcatccttc cggacaccag ctggtggtga ttcgcggtgc gggagatatc
    65881 gccagtggtg tggcgctacg tctgtatcat gcgggtttta aagtgatcat gctggaagtg
    65941 gaaaaaccga cagtgattcg ttgtaccgtg gcgtttgccc aggccgtgtt cgatggcgaa
    66001 atgacggtcg aaggcgtcac tgctcgcctg gcaaccagct ctgcggaagc gatgaaactt
    66061 accgaacgcg gattcatccc tgtgatggta gatcccgcct gttcattgct tgatgaactg
    66121 aaaccgcttt gcgtggtgga cgctattctg gcgaaacaga atttgggaac gcgggcagat
    66181 atggcaccag taacaatcgc gcttgggccg ggctttactg ctgggaagga ttgtcatgcg
    66241 gtaattgaaa caaatcgcgg gcactggctc ggtcaggtga tttactctgg ttgtgcgcag
    66301 gagaataccg gtgttcctgg caatattatg gggcatacca cccgacgggt gatccgtgct
    66361 cctgctgcag gcattatgcg atccaacgtg aaattaggcg atctggtgaa agagggcgat
    66421 gtgattgcct ggattggtga gcatgaaatt aaagcaccgt tgacggggat ggtgcgtggc
    66481 ttgttgaacg acggcctggc agtggttggt ggttttaaaa ttggtgatat cgatcctcgt
    66541 ggtgaaaccg ctgatttcac cagcgtttct gataaagccc gggcgattgg cggcggcgta
    66601 cttgaggcgt taatgatgtt gatgcatcag ggcgtgaaag cgacaaaaga agtgctggaa
    66661 gtggcttaaa tgaaaatggc cggatggtat tacccacccg gcctgagact gatgacaaac
    66721 gccaaattgc ctgatgcgct acgcttatca ggcctacaag tgcattgcaa tctattgaat
    66781 ttgcgatgtt ttgtaggacg gataaggcgc tcgcgccgca tccggcatca acaaagcgca
    66841 cttacttgac aatctttggc cggatggtat tacccacccg gcctttaaat tactgacaaa
    66901 tcaccgttcc tgttttgcct tcaatccctt ctttcgcttt gctcaatacg gtaatcagtg
    66961 cttcacgacc tgcgcgagag cgagcaaacg aggcggcagc ttccacttta ggcagcatag
    67021 aacctttcgc aaaatgacct tcttcgataa agcgttcagc atcacttaat gacaggcgat
    67081 cgagccactg ttcattctct ttaccgaagt taatggccac tttttctacc gcagtcagaa
    67141 tgatcagcat atcggcatca atcatttctg ctaaacgggc gctggcccag tctttatcga
    67201 taaccgcgct ggcaccacgc agatggttac cttcacgaat aacagggata ccaccaccgc
    67261 caacggtgat caccacctga cctgcatcta ccagagcttt aaccgtttct ttttcaatga
    67321 tatcaaccgg ttttggcgag gcaacaacac ggcgatagcc gcgaccagca tcttctttca
    67381 gagtgtaacc ctgttttgtc agttgctctg cttcttgctc ggtaaagaac gaaccgatcg
    67441 gcttggtcgg gttgaggaat gccggatcgt tagcgtcgac ttcgacctga gtaaccagcg
    67501 ttgctacagg tttattaatg ccccgagaaa gcagttcttc tcttaaagcg ttttgtagat
    67561 cgtaaccaat ataaccctgg cttaatgcaa cacagacaga catcggcagc attggcgagt
    67621 gcgcttcagt ttttgcggca gcttcaaagg cctggttaat catgcccacc tgcggcccgt
    67681 taccatgagt gacgataact tcatgtccct gggcaattaa atcaacaatt gcctgagaag
    67741 taatttttac cgctttcatt tgcccggcca ggtcatcacc cagcgcattc ccacccaggg
    67801 cgagaacaat tttcttactc atttttcctc actcatcatt cttgattgtg tatagatccc
    67861 tggcagtggt tagagcacgg gagggacaaa cggtttgcgt cgcaggaaac gacctcgccc
    67921 ggctttgcct gtaaaggtgc cgtcacagaa aatcgtttca ccacgggata aggttctgac
    67981 aatcgcgccc tgacaggtaa aaccctccca tggcgagtag tcggcgttgt cgtggagatg
    68041 gcgatgctga atttgttggc tctgacgtgg gtcgataatc accacgtcgc cgtcggaacc
    68101 gggcgctaat aatccttttt gcggccacag gccaaacagc ctggcgggca ttgcgctggt
    68161 taattcaaca aagcgttccg gtgttatacg tcccgtcatc acgccactgg aaaacagtaa
    68221 ctgcatgcgg ttttccacac cgggtaagcc atttgggcag cgactgaaat cgcctttaga
    68281 aatttgcagg cgttgagcca tcgagaaggt gcagtgatcg gttgccacca cgtcaatcgc
    68341 accatcgctg atgccacacc acagtttgtc ctgctcgcgt acgttacgca gcggtgggct
    68401 aagaatgaac ttcatgccat cttctgtatc gtaactgcgt tcgtccaaca ggagatattg
    68461 tgggcaggtt tcaacccaga ctggctggtg attcgcacgg gcaagacgca gataatccag
    68521 acctaagccg ttagacaggt gcacgatata gagcggggcg ttaccggcaa tttgtgccag
    68581 gttaatcatg cgggcgatgg cttccgcttc gcattccaga gggcgactca aggcgtgata
    68641 gcgcggcgcg gttaaccctg cggcgataaa ctccgcccgc ttgctggcga tagccgcatc
    68701 attttccggg tgcacggtgg tcagcgcgcc ggattcatgc agacggcgta atgcctgcaa
    68761 aacctcgtca tcgttgagtt tgtattgata ggttaaatag agtttaaaac tgctcagtcc
    68821 ttcctcgacc atcatcggga tttcgtcgag gattgcgtga ttaatgtgct ggatcacacc
    68881 gtgaaagctg taatcgatga ccgctttatg ggcggcataa ccacgataaa cctccagttg
    68941 atggcgtaac cgacagccgt ttgggccaaa tcccatatgg tcaataatgg ttgttgtacc
    69001 gccacacgca gctgcgcggg taccggtaaa aaaatcatca caactgcgcg cgatgccgac
    69061 atcaatattg aaatgcgtat ggacatccac gccgccaggg aaaacgtaac agccagtggc
    69121 atcaatttct tcatacggga gctgcggcga aatattgttg cccaactggc gaacaatccc
    69181 gctttcaatc agcaaatcct gtttggcttg tccatctgcg ttaacgacag tgccgttttt
    69241 gatcaatacg cgcatagcaa actccaaaaa ccctccggcg aaccggaggg gaataagtga
    69301 agagtaatta ttcggttgcc agccagctta acgggattgc agcgtacatc gcggcacagg
    69361 tcaccaggtg agatttccag gttttttcgt taggtgcatg cgcttcaggt tctttacccg
    69421 ggccaaagcc gatcaccgga atgccgtgac ggcccatgat agatacgccg ttagttgaga
    69481 aggtccactt atcaacaacc ggcgctttgc caaacagccc ttcgtaggca ttcaccagtg
    69541 ctttaacggt gaagtgatct tcttccactt tccaggtcgg gaagtagcat tcggttgggt
    69601 aaaccaggcc agtccaggac ggacggtcgt agttgtacat agaaacaacc gcgttagctt
    69661 tctgtactgc aggcagggcg cggatttcgt ccagcgcgcc ttcccaggtt tcgccccagg
    69721 tcagacggcg gtcaatagag acggcgcagc tatctgctac agcgcaacgg cttggggatg
    69781 tgaagaagat ttcagaaacg gtgagggtgc ctttgccgag gaattcgtca taacccagac
    69841 gttgggagag ttcttgtaat tcgccaagaa tcggacccat tttgaaaatg gcgttgtcac
    69901 cgcgttctgg cgcagaaccg tggcagctaa caccctgaac atcaatacga atttccatac
    69961 gaccgcgctg accacggtat acctggcagt cggttggttc ggtactgacc acaaattccg
    70021 ggcgaatgcc ggattgttca ataatgtact gccagcacag accgtcgcag tcttcttcct
    70081 gcacagtacc ggtaaccagc agggtatatt catcttccag accgaggtct ttaatgattt
    70141 taccggcata aaccatagat gccatgccgc cttcctggtc ggaagtaccg cgaccaccga
    70201 ttagctcatc agtttccatg ccttcgtacg gatcgaagtc ccagtttttg atgttgccaa
    70261 tgccgacggt atcgatatga gcgtccattg ccaccagacg cgggccgtgg ccgatataac
    70321 cgagaacgtt gcccatcggg tcgatttcaa ctttatcgaa gccgactttt tccatctctt
    70381 ctttaatacg atgtactacg cgtttttcgt cgcagctttc actgggaata gcaaccatgt
    70441 cgcgcaggaa gcgagtcatg tccgcctggt aatcttttgc tttttcaaga atcagtttga
    70501 atggaatatt cttagccatt atttttctcc agatttttta ctccccgttt tgcagggagt
    70561 agttccaata gggtgattaa ggtgctacag cgtgtttgcc ttcccagaca acttcgcggt
    70621 agtgcttcac gtcggtgtcg ccttcagtac tgataaccag cactacggca tctttgttca
    70681 gcgccagttt ttccatcagg ctttgacgtt gcgggtgata atgaaccgct gcgagaacgc
    70741 ccaaaccgac agcgccggat tcaccggaga tgatgcgcgg gtcgttgccg tacggattac
    70801 ccagcacgcg catacctaat gcggcaacgc tgtcctggca ggagatgaat tgggtggcgc
    70861 agttacgtag gatttcccag cccagcgggt taggttcgcc acaggccagg cccgccatga
    70921 tggtggccat atcaccgcca acgttgacga tgtcgccttt gacgccggag cgataaatac
    70981 agtcagcttt gtcaggttca acaataatgc tgtgcagatt ttgcgggctg tagacgtcga
    71041 ccagataacc cagcacacca ccggccattg ctccgacacc ggcttgcagc agaacgtgcg
    71101 tcggggttac gcccatttca cgcatttgct cgacggcttc atctgccagg gttgcgtagc
    71161 cttgcatgat ccaggttggg attttggtgt aaccttccca tgccgtgtcc tgtaccactt
    71221 cccagccgtg ctgctgcgcg tgttgcatgg tcaggcgaac ggtatcgtca tagttcatat
    71281 ctgtgacgat gcactcggca ccgaggttca gaatggcgtc aacgcgttcc tgagcagaac
    71341 ctttcggcat gtaaatcacc gcattctgtc cgagttgctg tgctgcccac gccacaccgc
    71401 gcccgtggtt gccgtcggtg gtggtcgcga aagtcatttt ttcgccgatg gtatttttca
    71461 ggtgctcaaa tgacagcgtt tcgatatcaa gatgatattt ttcacacaat aattgagcga
    71521 tggcgtacgc accgccaagc attttgaagg cgttcagacc gaatcgtttt gattcgtctt
    71581 tgacgagaat tttcttcaca ccaaaaaggt ttgcgagatc gtccagcgca caaagcggtg
    71641 ttgggcgata accagctatt ttctggtgga actggcgcgc cagtttggct tggctttgcg
    71701 aaaagagcgg tgatgtttcg ccgttgaaaa atttgttatc ggcgatatca atcttcaatg
    71761 agaaaacgga catatcctat ccttttggcc tctggaaata gagggccgac cagctgatcg
    71821 gcccgagggg ttatttcacg cgttcttgcg cttctttcag cagttgctcg agcagtgcac
    71881 ctggtttggc gtatttacgg gacaggatca tcgcggcgat gatataaggc ttccagctgg
    71941 cttctttgta ggtagcgata cggtattttt cgaatacgcc ttcagtcact tcaccttctt
    72001 tacaggatac gccgctgata tcagctggca ggcagtgcat gtacagggct tcgccatcac
    72061 gggtcagttc catcatctct tcagtacaat gccagtcttt gtgttgcgcg ttctgtgcca
    72121 gacactgttt ttccagtgct tttaagcctt cgtgatcgtt cgcacgcagc aattcagtac
    72181 gctcttccat cactttgtaa ggtgcccatg acttcggata aacgatgtct gcgtctttga
    72241 aggcttcttc catgctggtg acctgacgga agctaccacc ggaggcttta gcattgtttt
    72301 tcgccacttc aaccacatcc gggatcaggt cgtagccttc cggatgggcc agggtgacat
    72361 ccataccgaa gcgagtcatc agaccgatga tgccttgtgg tacagagagc ggtttgccat
    72421 agcttggaga gtaggcccag gtcatggcga tttttttacc tttcaggttt tccagtgaac
    72481 caaagtgttc acgtaaccac gcgaggtcag ccattgactg agtcgggtgg tcaatatcgc
    72541 attgcaggtt cactaaagcc ggacgctgtg gcagtacacc ctgcttgtaa ccgtcatcaa
    72601 gtgcagcgcc aacttcacgc atataggcgt tgcctgcgcc cagatacata tcgtcgcgaa
    72661 taccaatagc gtcggcgcag aaggagatca tattggcggt ttcacgcacg gtttcgccgt
    72721 gagcgatttg tgatttgcct tcgtcgagat cttgttgtgc aaggccgagc aggttaagcg
    72781 cggaagcata agagaagcgg gtacgggtgg agttgtcgcg gaatacggaa atacctaatc
    72841 cactattaaa gactttggtt gagatgtttt cagcacgcag tgcttttaat gctgcggcaa
    72901 cgtccagtac ttgtttcagt tcatctggcg tctgttccca cgttaacaaa aaatctttct
    72961 cgtgaaggtg agaggtcagc gaattgatat ccttaatcag ctcattaaca gttttcatca
    73021 taaagtcccg gtagtggaat gacaggcaaa gtggtttatt tcactgtgac ttgccaaagt
    73081 gattggtgtt ttcacagcta tacctgggac taacaataag tgtgccagaa ttatccgcgc
    73141 ggctgcaaaa gactaatttg acggataatg tgtggggttg atcacaaatt gatgccagct
    73201 tctggttgtt tgtaaaatac gaaaacgttt ggcttttttt atcgcgttat ttatttgctg
    73261 ctaagtatca gaaataagat aaaggggcag gctatctctc ttcagtttga taaactcacc
    73321 gccgtaattc tcatattgat atag
//
LOCUS       DAPHBJ010000005        66963 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.005, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000005 DAPHBJ010000000
VERSION     DAPHBJ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.005"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..458)
                     /gene="folK"
                     /locus_tag="RHZ76_000287"
     CDS             complement(<1..458)
                     /gene="folK"
                     /locus_tag="RHZ76_000287"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDV2810991.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILTVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGEMLQNRLTILNT"
     gene            complement(455..1873)
                     /gene="pcnB"
                     /locus_tag="RHZ76_000288"
     CDS             complement(455..1873)
                     /gene="pcnB"
                     /locus_tag="RHZ76_000288"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HDV2810992.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNISDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            complement(1912..2838)
                     /gene="gluQRS"
                     /locus_tag="RHZ76_000289"
     CDS             complement(1912..2838)
                     /gene="gluQRS"
                     /locus_tag="RHZ76_000289"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HDV2810993.1"
                     /translation="MLPPYFLFKEMTDTHYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKSDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWRLTAVPESAIVNSTFSNASC"
     gene            complement(2875..3330)
                     /gene="dksA"
                     /locus_tag="RHZ76_000290"
     CDS             complement(2875..3330)
                     /gene="dksA"
                     /locus_tag="RHZ76_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HDV2810994.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3508..4212)
                     /gene="sfsA"
                     /locus_tag="RHZ76_000291"
     CDS             complement(3508..4212)
                     /gene="sfsA"
                     /locus_tag="RHZ76_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDV2810995.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     FRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILTYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4227..4757)
                     /gene="thpR"
                     /locus_tag="RHZ76_000292"
     CDS             complement(4227..4757)
                     /gene="thpR"
                     /locus_tag="RHZ76_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HDV2810996.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRAKHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4831..7260
                     /gene="hrpB"
                     /locus_tag="RHZ76_000293"
     CDS             4831..7260
                     /gene="hrpB"
                     /locus_tag="RHZ76_000293"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HDV2810997.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQILLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAILVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDRPAVDNESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7455..9989
                     /gene="mrcB"
                     /locus_tag="RHZ76_000294"
     CDS             7455..9989
                     /gene="mrcB"
                     /locus_tag="RHZ76_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HDV2810998.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSSNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10209..12452
                     /gene="fhuA"
                     /locus_tag="RHZ76_000295"
     CDS             10209..12452
                     /gene="fhuA"
                     /locus_tag="RHZ76_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HDV2810999.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSDPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12503..13300
                     /gene="fhuC"
                     /locus_tag="RHZ76_000296"
     CDS             12503..13300
                     /gene="fhuC"
                     /locus_tag="RHZ76_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HDV2811000.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSCCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAALVSFVY"
     gene            13300..14190
                     /gene="fhuD"
                     /locus_tag="RHZ76_000297"
     CDS             13300..14190
                     /gene="fhuD"
                     /locus_tag="RHZ76_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HDV2811001.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14187..16169
                     /gene="fhuB"
                     /locus_tag="RHZ76_000298"
     CDS             14187..16169
                     /gene="fhuB"
                     /locus_tag="RHZ76_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HDV2811002.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITITTLWALPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16327..17607)
                     /gene="hemL"
                     /locus_tag="RHZ76_000299"
     CDS             complement(16327..17607)
                     /gene="hemL"
                     /locus_tag="RHZ76_000299"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDV2811003.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTSRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17832..19253
                     /gene="clcA"
                     /locus_tag="RHZ76_000300"
     CDS             17832..19253
                     /gene="clcA"
                     /locus_tag="RHZ76_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HDV2811004.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19277..19342
                     /locus_tag="RHZ76_000301"
     CDS             19277..19342
                     /locus_tag="RHZ76_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2811005.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19335..19679
                     /gene="erpA"
                     /locus_tag="RHZ76_000302"
     CDS             19335..19679
                     /gene="erpA"
                     /locus_tag="RHZ76_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HDV2811006.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19726..20349)
                     /locus_tag="RHZ76_000303"
     CDS             complement(19726..20349)
                     /locus_tag="RHZ76_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HDV2811007.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20387..21187)
                     /gene="btuF"
                     /locus_tag="RHZ76_000304"
     CDS             complement(20387..21187)
                     /gene="btuF"
                     /locus_tag="RHZ76_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HDV2811008.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21180..21878)
                     /gene="mtnN"
                     /locus_tag="RHZ76_000305"
     CDS             complement(21180..21878)
                     /gene="mtnN"
                     /locus_tag="RHZ76_000305"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDV2811009.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21962..23479
                     /gene="dgt"
                     /locus_tag="RHZ76_000306"
     CDS             21962..23479
                     /gene="dgt"
                     /locus_tag="RHZ76_000306"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HDV2811010.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23609..25033
                     /gene="degP"
                     /locus_tag="RHZ76_000307"
     CDS             23609..25033
                     /gene="degP"
                     /locus_tag="RHZ76_000307"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HDV2811011.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25187..26344
                     /gene="cdaR"
                     /locus_tag="RHZ76_000308"
     CDS             25187..26344
                     /gene="cdaR"
                     /locus_tag="RHZ76_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HDV2811012.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKTMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26433..26819)
                     /locus_tag="RHZ76_000309"
     CDS             complement(26433..26819)
                     /locus_tag="RHZ76_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HDV2811013.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26981..27793)
                     /gene="yaeI"
                     /locus_tag="RHZ76_000310"
     CDS             complement(26981..27793)
                     /gene="yaeI"
                     /locus_tag="RHZ76_000310"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HDV2811014.1"
                     /translation="MISRRRFLQATAATIATSSGFGYMHYCEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMSLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSAGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLVLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(27847..28671)
                     /gene="dapD"
                     /locus_tag="RHZ76_000311"
     CDS             complement(27847..28671)
                     /gene="dapD"
                     /locus_tag="RHZ76_000311"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HDV2811015.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28702..31374)
                     /gene="glnD"
                     /locus_tag="RHZ76_000312"
     CDS             complement(28702..31374)
                     /gene="glnD"
                     /locus_tag="RHZ76_000312"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HDV2811016.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECILEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGNPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31436..32230)
                     /gene="map"
                     /locus_tag="RHZ76_000313"
     CDS             complement(31436..32230)
                     /gene="map"
                     /locus_tag="RHZ76_000313"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDV2811017.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32598..33323
                     /gene="rpsB"
                     /locus_tag="RHZ76_000314"
     CDS             32598..33323
                     /gene="rpsB"
                     /locus_tag="RHZ76_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDV2811018.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33581..34432
                     /gene="tsf"
                     /locus_tag="RHZ76_000315"
     CDS             33581..34432
                     /gene="tsf"
                     /locus_tag="RHZ76_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDV2811019.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34579..35304
                     /gene="pyrH"
                     /locus_tag="RHZ76_000316"
     CDS             34579..35304
                     /gene="pyrH"
                     /locus_tag="RHZ76_000316"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDV2811020.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35596..36153
                     /gene="frr"
                     /locus_tag="RHZ76_000317"
     CDS             35596..36153
                     /gene="frr"
                     /locus_tag="RHZ76_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDV2811021.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36245..37441
                     /gene="ispC"
                     /locus_tag="RHZ76_000318"
     CDS             36245..37441
                     /gene="ispC"
                     /locus_tag="RHZ76_000318"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDV2811022.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37630..38388
                     /gene="ispU"
                     /locus_tag="RHZ76_000319"
                     /gene_synonym="uppS"
     CDS             37630..38388
                     /gene="ispU"
                     /locus_tag="RHZ76_000319"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HDV2811023.1"
                     /translation="MLSAIQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38401..39258
                     /gene="cdsA"
                     /locus_tag="RHZ76_000320"
     CDS             38401..39258
                     /gene="cdsA"
                     /locus_tag="RHZ76_000320"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDV2811024.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39270..40622
                     /gene="rseP"
                     /locus_tag="RHZ76_000321"
     CDS             39270..40622
                     /gene="rseP"
                     /locus_tag="RHZ76_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HDV2811025.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40652..43084
                     /gene="bamA"
                     /locus_tag="RHZ76_000322"
     CDS             40652..43084
                     /gene="bamA"
                     /locus_tag="RHZ76_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDV2811026.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            43206..43691
                     /gene="skp"
                     /locus_tag="RHZ76_000323"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             43206..43691
                     /gene="skp"
                     /locus_tag="RHZ76_000323"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HDV2811027.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43695..44720
                     /gene="lpxD"
                     /locus_tag="RHZ76_000324"
     CDS             43695..44720
                     /gene="lpxD"
                     /locus_tag="RHZ76_000324"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDV2811028.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44825..45280
                     /gene="fabZ"
                     /locus_tag="RHZ76_000325"
     CDS             44825..45280
                     /gene="fabZ"
                     /locus_tag="RHZ76_000325"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDV2811029.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45284..46072
                     /gene="lpxA"
                     /locus_tag="RHZ76_000326"
     CDS             45284..46072
                     /gene="lpxA"
                     /locus_tag="RHZ76_000326"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HDV2811030.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            46072..47220
                     /gene="lpxB"
                     /locus_tag="RHZ76_000327"
     CDS             46072..47220
                     /gene="lpxB"
                     /locus_tag="RHZ76_000327"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDV2811031.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            47217..47813
                     /gene="rnhB"
                     /locus_tag="RHZ76_000328"
     CDS             47217..47813
                     /gene="rnhB"
                     /locus_tag="RHZ76_000328"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDV2811032.1"
                     /translation="MIEFVYPHTHLVTGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPYEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPSMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47850..51332
                     /gene="dnaE"
                     /locus_tag="RHZ76_000329"
     CDS             47850..51332
                     /gene="dnaE"
                     /locus_tag="RHZ76_000329"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDV2811033.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51345..52304
                     /gene="accA"
                     /locus_tag="RHZ76_000330"
     CDS             51345..52304
                     /gene="accA"
                     /locus_tag="RHZ76_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDV2811034.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLSVPTICTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52403..54544
                     /gene="ldcC"
                     /locus_tag="RHZ76_000331"
     CDS             52403..54544
                     /gene="ldcC"
                     /locus_tag="RHZ76_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HDV2811035.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALLAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            54601..54990
                     /locus_tag="RHZ76_000332"
     CDS             54601..54990
                     /locus_tag="RHZ76_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV2811036.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            55055..56353
                     /gene="tilS"
                     /locus_tag="RHZ76_000333"
     CDS             55055..56353
                     /gene="tilS"
                     /locus_tag="RHZ76_000333"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDV2811037.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQVRYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTPQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVAGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGVDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYDETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(56402..56662)
                     /gene="rof"
                     /locus_tag="RHZ76_000334"
     CDS             complement(56402..56662)
                     /gene="rof"
                     /locus_tag="RHZ76_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HDV2811038.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56649..56849)
                     /locus_tag="RHZ76_000335"
     CDS             complement(56649..56849)
                     /locus_tag="RHZ76_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HDV2811039.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            57015..57560
                     /locus_tag="RHZ76_000336"
     CDS             57015..57560
                     /locus_tag="RHZ76_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HDV2811040.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57557..57979
                     /gene="arfB"
                     /locus_tag="RHZ76_000337"
     CDS             57557..57979
                     /gene="arfB"
                     /locus_tag="RHZ76_000337"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDV2811041.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKALRGKVRSGRE"
     gene            57993..58703
                     /gene="nlpE"
                     /locus_tag="RHZ76_000338"
                     /gene_synonym="cutF"
     CDS             57993..58703
                     /gene="nlpE"
                     /locus_tag="RHZ76_000338"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HDV2811042.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEGNPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(58903..59727)
                     /locus_tag="RHZ76_000339"
     CDS             complement(58903..59727)
                     /locus_tag="RHZ76_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HDV2811043.1"
                     /translation="MDKPKAYYRLLLPSFLLLSACTVDISQPDSSATAVDAGAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKLGIYIKAGRFVGLTR"
     gene            complement(59780..61498)
                     /gene="proS"
                     /locus_tag="RHZ76_000340"
     CDS             complement(59780..61498)
                     /gene="proS"
                     /locus_tag="RHZ76_000340"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDV2811044.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(61609..62316)
                     /gene="tsaA"
                     /locus_tag="RHZ76_000341"
     CDS             complement(61609..62316)
                     /gene="tsaA"
                     /locus_tag="RHZ76_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HDV2811045.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(62313..62717)
                     /gene="rcsF"
                     /locus_tag="RHZ76_000342"
     CDS             complement(62313..62717)
                     /gene="rcsF"
                     /locus_tag="RHZ76_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HDV2811046.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62835..63650)
                     /gene="metQ"
                     /locus_tag="RHZ76_000343"
     CDS             complement(62835..63650)
                     /gene="metQ"
                     /locus_tag="RHZ76_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HDV2811047.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(63690..64343)
                     /gene="metI"
                     /locus_tag="RHZ76_000344"
     CDS             complement(63690..64343)
                     /gene="metI"
                     /locus_tag="RHZ76_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HDV2811048.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(64336..65367)
                     /gene="metN"
                     /locus_tag="RHZ76_000345"
     CDS             complement(64336..65367)
                     /gene="metN"
                     /locus_tag="RHZ76_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HDV2811049.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKYDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            65555..66127
                     /gene="gmhB"
                     /locus_tag="RHZ76_000346"
     CDS             65555..66127
                     /gene="gmhB"
                     /locus_tag="RHZ76_000346"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDV2811050.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
     gene            66491..>66963
                     /locus_tag="RHZ76_000347"
     rRNA            66491..>66963
                     /locus_tag="RHZ76_000347"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        15822 a        16696 c        18469 g        15976 t
ORIGIN      
        1 gtattcagga tagtaagtct attttgcaac atctccccat caggaaacac caactccggc
       61 gcgatttcaa acagcggcca cagcataaat ccacgattct tcatatcgta gtgcggaacg
      121 gtcaggcgtt cagtatttat cacttcatta ccaaacagca tgatgtcgag atccagcgtg
      181 cgtggtcccc agcgttcagc tttgcggacg cgaccttgct gcaattcaat acgctgtgtg
      241 tgattgagta gctcttcagg tgcaagagag gtttccagcg ccacggctgc gtttaagtaa
      301 tcgggttgat cttgcggccc cagcggtggg gtgcggtaaa acgaagaaac ggtaagaatg
      361 tggctttcag ggatatcgcc taatgctttc agggcagcat tgacctgctc cagcggagag
      421 gccagattgc tgcctatggc aatatacgcc actgtcatgc ggtaccctca cgacgtggtg
      481 cgcgtttgcg tggacgacga gtacgacgac gcggtgacgg ttcttcatcc agctcgttga
      541 gcatcccttt ttggtctggt ggcgcggaaa cctggaactc accccaccat ttcaccagac
      601 gctgcagttc agcgttacgc tcaacttcag ctcgcaaggc caacaggtca taagccgcac
      661 ggaacttagg atgctccagc agtttccatg cgcgtttacc ctgacgacgg gacatacgca
      721 actgcaactg ccagatatcg cgggttaatg tcgtcagacg tttcgggatt gccagtgaac
      781 ggcaggcttc gtccagcacg tcgttcatcg ccagcgcgaa agcgtcgtga taggtcaggc
      841 cgctttcctg ggcgatcttc tgtgccgtct ccagcagtgg gtaccagaac atggcggcaa
      901 acaggaacgc cgggttcacg cgcatatcgt tatggatacg cgtatcggta ttcttcagca
      961 cctgttcaat gatccgctcc atcgggctgt cgccattttc cgtgaagtag cgggtaatgg
     1021 tcgggaacag cggctggaac agatgatatt cacacaacag cttataggtt tcgtaaccgt
     1081 agcccgcttg tagcagttta agcgattctt caaacaggcg tgccggtggg atatcgttca
     1141 gcagggtagc gaggcgaggg atcggttctg cggtttccgg gctgatgcgc atacccaatt
     1201 tggcggcaaa acgtaccgcg cgcagcatac gtaccggatc ttcacggtag cgcgtttccg
     1261 ggttaccaat cagacggata acgccgtcct tcagatcctt catgccgcca acgtaatcac
     1321 ggacggtaaa atccgctacg ctgtaataca ggctgttgat agtgaaatcg cggcgctggg
     1381 cgtcttcttc gatggagccg aaaatgttgt cgcgcagcaa catgccgttt tgcccgcgtt
     1441 gggaggtcgt gcggtcgctg atgttacctt cgtggtgtcc acggaaggtc gcaacttcga
     1501 taatctccgg gccaaacatc acatgagcca gacggaaacg gcgacccaca aggcggcagt
     1561 tacggaacag tttgcgcacc tgctcaggcg tggcgttagt ggttacgtca aagtctttcg
     1621 gctttttgcc aagtaacagg tcgcgcacgc cgccgccaac cagccaggct tcgtatcccg
     1681 ctttattgag cctgtacatt accttcaggg cattttcact gatatctttg cgggaaatag
     1741 catgctgctc acgcgggatc accgtcacct gtggacgggc gactgcctgt tcagcctcgc
     1801 tttcctcgcg gcttagcacc ttgcggcaaa aattagcgac tcgggtaaaa atagtacacc
     1861 tcggtagtgt caaacatcat tcaggacaaa aaaatagcgg ctaatcatag ctcagcatga
     1921 cgcatttgag aatgttgaat ttacaattgc tgactcaggc acggcggtaa gccgccagtt
     1981 tttgacggct gactggagga tttgctcgac gctgaaatcc tgccagtgtg cttctgcctg
     2041 ctgacccaga aattgaagtg ccgcgattag taccgggcgt ggatcgcttt tcggcaacgc
     2101 aggcgcatga ttctgcttgg aaagtttagc gccttgtgga ttaagcgcca gcggcagatg
     2161 aatgtaatct ggcactttcc agccaaaaag ctggtacagc gagatttgcc gcactgtcgg
     2221 ttcgatcaaa tctgccccgc ggacaatttc gctcacgccc tggaagtgat catccaccac
     2281 caccgccagg ttataggcaa acaaaccatc acggcgatga ataataaaat cttcccgtgc
     2341 cagtttttcg tcggcgtgaa taatgccgcg cagctggtca gtaaattgcg tgaccggatg
     2401 ctgctggcgg atacgcactg cggcgttgtc tggtccatga tgcaacaccc ggcaatgacc
     2461 gtcgtaaata ccgccaatgc tttgaatacg cgcacgcgtg caggtgcagt aataacttag
     2521 cccttgctca tgtaaccagg cgagtgcttc acgataggcg tcgtgacgtt gcgattgcca
     2581 gagaacatcg ccgtcccagt gcagaccgta atgttccagc tggcgcagga tagtttctgc
     2641 ggcaccggga acttcacgag gcgggtcgat atcttctata cgtaccagcc agcgaccttg
     2701 ccgggcgcga gcctgcaaat agctgccaag cgcagcaatc agagaaccaa aatgaagctc
     2761 gccggaagga gagggggcga agcggccaat atagtgtgtg tctgtcatct ctttgaacaa
     2821 aaaataaggc gggagcattt cccgcctgtg gtaaacgtga tggaacggct gtaattagcc
     2881 agccatctgt ttttcgcgaa tttcagccag cgttttgcag tcgatgcaca gatcggctgt
     2941 cgggcgcgct tccagacggc gaataccaat ttcaacaccg caggattcgc agtagccgaa
     3001 atcttcgtct tccacttttt tcagcgtctt ctcgatcttt ttgatcagct tacgctcgcg
     3061 atcgcggtta cgcagttcga ggctgaactc ttcttcctgg gctgcacggt ctaccgggtc
     3121 cgggaagttg gctgcttcat cctgcatatg tgtaacggtg cgatcgactt catccctgag
     3181 ttgattacgc catgcttcca gaatacgacg gaagtgcgcc agctgggctt cattcatata
     3241 ctcttcgccc ggcttctcct gatatggttc caccccagcg atggcgagaa tactcaggga
     3301 cgatgtttta cggttttgcc cttcttgcat gttgcttctc cttaacacgc actatcgatc
     3361 cccatgttcg ggggaaaaat gaggccgcta taaatagcag atgcttttcc ggatagcaat
     3421 tatctaaacg taacacttga caactgtgtg aggaaaagcg tatttgcgca cgcgaccaga
     3481 atgtaaatta accagttact tactttacta caatgtaacc ggcagtgatt ttttaagagc
     3541 catgccttca gcagaaattt ccgctttgta agtcagaatt tctacccccc tctgttgagc
     3601 ttctgacaat agttgcgcgt atttctcatc gatgtggcgc gcgggtgaaa accgtgtaat
     3661 ggctgaatgc agcacggcga aaaagataac cgcacgctgg ccttcagccg ctacgctcat
     3721 caactcccga aagtgtttct gacctcgttc agtgaccgca tcgggaaaat atccctgttc
     3781 gttctccgct aacgtaaccg atttcacttc aatatagcag tctggacgcg aatccgcctg
     3841 caacataaag tcaatacggc tgcgttctgc gccgtatttt acttcgcttt tcagcgagct
     3901 atagcctgac agttctgaaa ttgattcatt aaggatagcc tctttcgtca acctgttagc
     3961 ccaaagcgtg ttgacgcaaa taaatgcgcc gctctggctt tgagttaatt cccaggtgtg
     4021 tgggtatttc cgtttggtgt tgtctgaagt cgaataccag acggtatcgc caggcgttgc
     4081 acaaccggtc atcgcacccg tattcgggca gtgtagcgtt aattcgcgac catcgggtgt
     4141 gatcacatcg gctaaaaaac gtttgtaacg ctgaattagc gtcgcgcgct gtagaggggg
     4201 agaaaattcc attgcgacaa tccttgttat tgcgttagcg cccagcgttt tagcggcgtg
     4261 tagcgtgtgc gtccacgggc aaacgaggag gcgtaaaggg tgaactccgt caccgcataa
     4321 gaccagttaa aacccggcgg cgggattgtc accgcctcgc tggcgtcgcg caataaggta
     4381 atatgtggat gaaacggacg attgctttga aaacaaccgc tgcgggcagc ctgtgaacgg
     4441 agcatattcg ccagctggat taagccgcgt ggaggctgac gcatccctaa ccacaccaca
     4501 cgcgaacgca gccattgtcc ggcgtcatca agcgtgagtg tgaaaccagg ttgacgaatc
     4561 cgtccggcta aaagagaaag cgccttctct ttctcggcgc tcacttcgcc taaaaacgcc
     4621 agagtcagat gcaaattatc ggcggcgacc ggacgtcccg cctcaggtgg gaagtgtttg
     4681 gcgcgccagt ggataatctg ttcgcggatt tctgcaggta agtcgatagc aaagaacaga
     4741 cgttgcggtt cagacatgtg aggcactcgg ttatgaatta ccgcgatgct acaatgtgcc
     4801 gcgaagaatg ttaaccctct ggagcgtttt gtgtcgtcgt tgcccgttgc tgccgtctta
     4861 cctgaattac ttaccgctct cgattgtgcg ccgcagatat tattaagtgc gccgaccgga
     4921 gccgggaaat caacctggct gccgctgcaa ctgctggcgc atcccggcat taacgggaaa
     4981 attatcctcc tggagccgcg tcgtctggcg gcgcgtaacg tcgcgcaacg gctggcggag
     5041 ctgcttaacg aaaagccagg cgataccgtt ggctaccgga tgcgtgcgca aaactgcgtc
     5101 gggccaaata cccgcctgga agtggttacc gaaggcgtgc tgacgcgcat gatccagcgt
     5161 gacccggaac tgagcggtgt tggattggtg atccttgatg aatttcatga gcgcagcttg
     5221 caggcggatt tggcgttggc gctgttactc gatgtgcaac aaggtctgcg tgatgacctt
     5281 aaactgctga ttatgtcggc tacgctggac aacgaccgct tgcagcaaat gctgccagaa
     5341 gcgcccgtcg tcatctcaga agggcgctcg tttccggttg aacgccatta tttaccgctg
     5401 cccgcgcatc agcgttttga cgaagccgtt gcggtagcca ccgccgaaat gctgcgtcag
     5461 gaaagcggat cattactgtt atttttacct ggcgtcggag aaattcagcg tgtgcaggaa
     5521 caactggctt cgcgcatcgg cagtgatgtg ttgctctgcc cgctgtatgg cgcgttgtcg
     5581 ctgaacgatc agcgaaaagc gatcctcccg gcaccgcaag ggatgcgcaa agtggtgctg
     5641 gcgaccaata ttgctgaaac cagtttaacc atcgaaggta ttcgtctggt ggtggattgt
     5701 gcccaggagc gtgtggcgcg ttttgagccg cgcacggggc ttacgcgact gattactcaa
     5761 cgcgttagcc aggcatccat gacgcagcgt gccgggcgcg ccgggcgtct ggagccgggt
     5821 atctgcctgc atttaatcgc caaagaacaa gcagaacgcg ctgccgcgca aagtgaaccg
     5881 gagatcttac aaagcgatct ttccggtttg ctgatggaat tactgcaatg gggatgcagc
     5941 gatccggcgc agatgagctg gctggatcaa ccgccagtag tgaatctaat ggccgcgaaa
     6001 cgtctgttac aaatgctggg ggcgctggac ggtgaacggc ttagtgcgca agggcaaaaa
     6061 atggcagcgc tgggtaacga tccgcgttta gcggcaattc tggttagcgc gaagaacgac
     6121 gacgaagctg ctaccgcggc aaaaattgcc gccattctcg aagagccgcc acggatgggc
     6181 aatagtgacc tgggcgtggc gttttcgcgc aatcaaccag cctggcagca acgtagtcag
     6241 caactgttaa aacgcttaaa cgtacgcggc ggtgaggcag acagttcgct tatcgcgccg
     6301 ctacttgccg gggcgtttgc cgatcgcatt gctcatcgcc gtgggcaaga tggacgctat
     6361 caactggcaa acggcatggg agcgatgctc gatgccgacg acgcgctaag ccgccacgaa
     6421 tggttgatcg caccgttatt attgcagggc agcgcctcgc cggatgctcg gattttactg
     6481 gcgctaccgg tcgatattga tgaattagta caacgctgtc cgcagctggt gcagcaatct
     6541 gacaccgtgg aatgggatga cgcgcaaggt acgctgaaag cctggcgtcg gctgcaaatc
     6601 ggtcagttga cggtgaaagt gcagccgtta gcgaaaccgt cagaagacga gttgcatcag
     6661 gcgatgctta atggcatccg tgataaaggt ttaagcgtgc tcaactggac ggcggaagcg
     6721 gaacagctac gcttgcgttt gttatgcgcc gcaaagtggt tgccggaata tgacaggcct
     6781 gcggttgata atgaaagttt gttggcgacg ctggaaacgt ggctgctgcc acatatgacc
     6841 ggcgtgcatt cactacgcgg cctgaaatca ctcgacattt atcaggcact acgcggatta
     6901 cttgattggg gaatgcagca acgtctggat agtgaattgc ctgcgcatta cactgtgccg
     6961 acgggaagcc ggatcgccat tcgttatcat gaagataacc cgcccgcgct ggcggtgaga
     7021 atgcaggaga tgtttggtga ggccaccaat ccgacgatcg cccaggggcg cgtgccgctg
     7081 gtgctggagt tgctttcccc tgcccaaagg ccattacaaa tcacacgaga tttgagcgcc
     7141 ttctggaaag gagcgtaccg tgaggtgcaa aaagagatga aagggcgtta tcccaaacat
     7201 gtctggccgg acgacccggc aaacactgca ccgacgcgac ggacgaaaaa gtattcgtaa
     7261 ggttggtttt ctccctctcc ctgtgggaga gggccggggt gagggcatca gcgcgcacgt
     7321 tcaccctcac cctaaccctc tcctcaaggg agaggggacc gatcgagcac aaattttgag
     7381 agatatcttc ttctgtcttg taacagaaga acagaaaatc gggcttttgc gcctgaatat
     7441 tgcggagaaa aagcatggcc gggaatgacc gcgagccaat tggacgcaaa gggaaaccga
     7501 cgcgtccggt caaacaaaag gtaagccgtc gtcgttacga agatgacgat gattacgacg
     7561 attatgatga ctatgaggat gaagaaccga tgccgcgcaa aggtaagggc aaaggcaaag
     7621 ggcgtaagcc tcgtggcaaa cgcggctggc tatggctact gctaaaactg gctatcgttt
     7681 ttgccgtgct gatcgccatt tacggcgttt atctcgatca aaaaattcgt agccgtattg
     7741 atggcaaggt ctggcaactg cctgcggcag tttatggccg aatggtcaat cttgagccag
     7801 acatgaccat cagcaagaac gagatggtga agctgctgga ggcgacccag tatcgtcagg
     7861 tgtcgaaaat gacccgtcct ggcgaattta ccgtgcaggc taacagcatt gagatgatcc
     7921 gccgtccgtt tgatttcccg gacagcaaag aagggcaggt gcgtgcgcgt ttgacctttg
     7981 atggcgatca tctggcgacc atcgtcaata tggagaacaa ccgtcagttc ggcttcttcc
     8041 gtcttgatcc gcgcctgatc accatgatct cctcgccaaa cggtgaacaa cgtctgtttg
     8101 tgccgcgcag tggtttccct gatttgctgg tggatacttt gctggcgaca gaagaccgtc
     8161 atttttacga gcatgacggc atcagcctct actccatcgg acgtgcggtg ctggcaaacc
     8221 tgaccgcagg tcgcacggtg cagggtgcga gtacgctgac gcaacagctg gtgaaaaacc
     8281 tgttcctctc cagcgagcgc tcttactggc gtaaagcgaa cgaagcctat atggcgttga
     8341 tcatggatgc gcgttacagc aaagatcgca ttcttgagct gtatatgaat gaggtgtatc
     8401 tcggtcagag cggcgacaac gaaattcgtg gcttcccgct ggcgagcctg tactactttg
     8461 gtcgcccggt agaagaattg agccttgacc agcaggcgct gttagttggc atggtgaaag
     8521 gggcgtccat ctacaacccg tggcgtaacc cgaaactggc gctggagcga cgtaatctgg
     8581 tgctgcgttt gctacaacaa cagcagatta tcgatggtga actgtatgaa atgctcagcg
     8641 cgcgtcctct gggcgtacag ccgcgcggtg gggtaatctc tccacagcca gcctttatgc
     8701 aactggtgcg tcaggagttg caggcgaaat taggcgataa ggtaaaagat ctgtccggcg
     8761 tgaagatctt cactaccttt gactcggtgg cccaggacgc ggcagaaaaa gccgccgtgg
     8821 aaggcattcc ggcactgaag aaacagcgta agctgagcga tctggaaacc gcgattgtgg
     8881 tcgtcgaccg ctttagtggt gaagttcgtg cgatggtcgg aggttctgag ccgcagtttg
     8941 cgggctacaa ccgtgcgatg caggcgcgtc gttcgattgg ttcccttgca aaaccagcga
     9001 cttatctgac tgccttaagc cagccgaaaa tctatcgcct gaacacgtgg attgcggatg
     9061 cgccaatcgc gctgcgtcag ccgaatggcc aggtctggtc accgcagaac gatgatcgtc
     9121 gttatagcga aagtggcaga gtgatgctgg tggatgcgtt gacccgttcg atgaacgtgc
     9181 cgacggtaaa tctggggatg gcactggggc tgcctgcggt tacggagacc tggatcaaac
     9241 tgggcgtgcc gaaagatcag ctgcatccgg ttccggcgat gctgctgggg gcgttgaact
     9301 taacgccaat cgaagtggcg caggcattcc agaccatcgc cagcggcggt aaccgtgcac
     9361 cgctttctgc gctgcgttcg gtaatcgcgg aagatggcaa agtgctgtat cagagcttcc
     9421 cgcaggcgga acgcgctgtt ccggcgcagg cggcgtatct gacactatgg accatgcagc
     9481 aggtggtaca acgcggtacg ggtcgtcagc ttggggcgaa atacccgaac ctgcatctgg
     9541 cagggaaaac agggactact aacaataacg tagatacctg gtttgcgggc attgacggca
     9601 gcacggtgac catcacctgg gtaggccgtg ataacaacca gccgaccaaa ctgtatggag
     9661 ccagcggggc aatgtcgatt tatcagcgtt atctggctaa ccagacgcca acaccgctga
     9721 atcttgttcc gccagaagat attgcagata tgggcgtgga ctacgacggc aactttgttt
     9781 gcagcggtgg catgcgtgtc ttgccggtct ggaccagcga tccgcaatcg ctgtgccagc
     9841 agagtgagat gcagcagcag ccgtcaagca atccgtttga tcagtcttct cagccgcagc
     9901 aacagccgca acagcaacct gctcagcaag agcagaaaga cagcgacggt gtagccggtt
     9961 ggattaaaga tatgtttggt agtaattaac gtctaagcgt gaaataccgg atggcgagtt
    10021 gccatccggt aaaataacat cccacccaaa gtattaaccc tttcttttca tctggttgtt
    10081 tattaaccct tcaggaacgc tcagattgcg taccgcttgc gaacccgcca gcgtttcgaa
    10141 tattatctta tctttataat aatcattctc gtttacgtta tcattcactt tacatcagag
    10201 atataccaat ggcgcgttcc aaaactgctc agccaaaaca ctcactgcgt aaaatcgcag
    10261 ttgtagtagc cacagcggtt agcggcatgt ctgtttatgc acaggcagcg gttgaaccga
    10321 aagaagacac tatcaccgtt accgctgcac ctgcgccgca agaaagcgca tgggggcctg
    10381 ctgcaactat tgcggcgcga cagtcagcta ccggcactaa aaccgatacg ccgattcaaa
    10441 aagtgccaca gtctatttct gttgtgaccg ccgaagagat ggcgctgcat cagccgaagt
    10501 cggtaaaaga agcgcttagc tacacgccgg gtgtctctgt tggtacgcgt ggcgcatcca
    10561 acacctatga ccacctgatc attcgcggtt ttgcggcaga aggccaaagc cagaataact
    10621 atctgaatgg cctgaagttg cagggcaact tctataacga tgcggtcatt gatccgtata
    10681 tgctggaacg cgctgaaatt atgcgtggcc cggtttccgt gctttacggt aaaagtagtc
    10741 ctggcggtct gttgaatatg gtcagcaagc gtccgaccac cgaaccgctc aaagaagttc
    10801 agtttaaagc cggtactgac agcctgttcc agactggttt tgactttagc gatgcgctgg
    10861 atgatgacgg cgtttactct tatcgcctga ccggtcttgc gcgttctgcc aatgcccagc
    10921 agaaagggtc agaagagcag cgttatgcta ttgcaccggc gttcacctgg cgtccggatg
    10981 ataaaaccaa tttcaccttc ctttcttact tccagaacga gccggaaacc ggttattacg
    11041 gctggttgcc gaaagaggga accgttgagc cgctgccgaa cggtaagcgt ctgccgacag
    11101 actttaatga aggggcgaag aacaacacct attctcgtaa tgagaagatg gtcggctaca
    11161 gcttcgatca cgaatttaac gacaccttta ctgtgcgtca gaacctgcgc tttgctgaaa
    11221 acaaaacctc gcaaaacagc gtttatggtt acggcgtctg ctccgatccg gcgaatgctt
    11281 acagcaaaca gtgtgcggca ttagcgccag cggataaagg ccattatctg gcacgtaaat
    11341 acgtcgttga tgatgagaag ctgcaaaact tctccgttga tacccagttg cagagcaagt
    11401 ttgccactgg cgatatcgac cacaccctgc tgaccggtgt cgactttatg cgtatgcgta
    11461 atgacatcaa cgcctggttt ggttacgacg actctgtgcc actgctcaat ctgtacaatc
    11521 cggtgaatac cgatttcgac ttcaatgcca aagatccggc aaactccgac ccttaccgca
    11581 ttctgaataa gcagaaacaa acgggcgttt atgttcagga tcaggcgcag tgggataaag
    11641 tgctggtcac cctgggcggt cgttatgact gggcagatca agaatctctt aaccgcgttg
    11701 ccgggacgac cgataaacgt gatgacaaac agtttacctg gcgtggtggt gttaactacc
    11761 tgtttgataa tggtgtaaca ccttacttca gctatagcga atcgtttgaa ccttcttcgc
    11821 aagttgggaa ggatggtaat attttcgcac cgtctaaagg taagcagtat gaagtcggcg
    11881 tgaaatatgt accggaagat cgtccgattg tagttactgg tgccgtgtat aatctcacta
    11941 aaacaaacaa cctgatggcg gaccctgagg gttccttctt ctcggttgaa ggtggcgaga
    12001 tccgcgctcg tggcgtagaa atcgaagcga aagcggcgct gtcggcgagt gttaacgtag
    12061 tcggttctta tacttacacc gatgcggaat acaccaccga tactacctat aaaggcaata
    12121 cgcctgcaca ggtgccaaaa cacatggctt cgttgtgggc tgactacacc ttctttgacg
    12181 gtccgctttc aggtctgacg ctgggcaccg gtggtcgtta tactggctcc agttatggtg
    12241 atccggctaa ctcctttaaa gtgggaagtt atacggtcgt ggatgcgtta gtacgttatg
    12301 atctggcgcg agtcggcatg gctggctcca acgtggcgct gcatgttaac aacctgttcg
    12361 atcgtgaata cgtcgccagc tgctttaaca cttatggctg cttctggggc gcagaacgtc
    12421 aggtcgttgc aaccgcaacc ttccgtttct aatttctctt ttggggcacg gatttccgtg
    12481 cccatttcac aagttggctg ttatgcagga atacacgaat cattccgata ccacttttgc
    12541 actgcgtaat atctcctttc gtgtgcccgg gcgcacgctt ttgcatccgc tgtcgttaac
    12601 ctttcctgcc gggaaagtga ccggtctgat tggtcacaac ggttctggta aatccactct
    12661 gctcaaaatg cttggccgtc atcagcctcc gtcagaaggg gagattcttc ttgatgccca
    12721 gccgctggaa agctggagca gcaaagcgtt tgcccgcaaa gtggcttatt tgccgcagca
    12781 gcttcctccg gcagaaggga tgaccgtgcg tgaactggtg gcgattggtc gttacccgtg
    12841 gcatggcgcg ttggggcgct ttggggcggc agatcgcgaa aaagtcgagg aagctatctc
    12901 gctggttggc ttaaaaccgc tggcgcatcg gctggtcgat agtctctctg gcggcgaacg
    12961 tcaacgggcg tggatcgcca tgctggtggc gcaggatagc tgttgtctgt tgctcgacga
    13021 accgacttcg gcgctggata tcgcccacca ggttgatgtg ctggcgctgg tgcaccgttt
    13081 aagtcaggag cgtggcctga cggtcattgc cgtgttgcac gatatcaata tggcggcacg
    13141 ctactgtgat tatctggtcg ccctgcgcgg tggtgaaatg attgctcagg gaacgcctgc
    13201 ggaaattatg cgcggcgaaa ctctcgaaat gatttatggc atcccgatgg gtattttgcc
    13261 gcatccggcg ggtgctgcac ttgtgagttt tgtttattga tgagcggctt acctcttatt
    13321 tcgcgccgtc gactgttaac ggcgatggcg ctttctccgt tgttatggca gatgaatacc
    13381 gcccacgcgg cggctattga ccccaatcgt attgtggcgc tggagtggtt gccggtggaa
    13441 ttactgctgg cgctcggcat cgtgccttac ggcgtggcgg ataccatcaa ctatcgcctg
    13501 tgggtcagcg aaccaccatt gccggactca gtgattgacg tcggtttgcg tacagaacct
    13561 aaccttgaac tgctgaccga aatgaaaccg tcgtttatgg tctggtcggc aggatatggc
    13621 ccttcaccag aaatgctggc acgtattgcg ccgggtcgcg gatttaactt cagtgacggc
    13681 aaacagccgt tggcgatggc gcgtaaatcg ctgacggaaa tggcagattt acttaacctg
    13741 caaagcgcag cggaaacgca tttagcgcaa tacgaagact ttatccgcag catgaaaccc
    13801 cgctttgtga agcgtggtgc acgcccgtta ctgctgacga cgcttatcga tccgcgccat
    13861 atgctggtct tcggtccaaa cagcttgttc caggaaattc tcgatgagta cggcatccca
    13921 aatgcctggc aaggggaaac caacttctgg ggcagtaccg ccgtcagtat cgatcgtctg
    13981 gcggcgtata aagacgttga tgtgctctgt tttgatcacg acaacagcaa agacatggat
    14041 gcgctaatgg caaccccgct gtggcaggcc atgccgtttg tccgcgccgg acgctttcag
    14101 cgcgtacctg cagtctggtt ttatggtgcg acgctatcgg caatgcactt tgtgcgcgtt
    14161 ctggataacg ccatcggagg taaagcgtga gtaaacgaat tgcgcttttc ccggcgttat
    14221 tgctggcgct gttggtgatt gtcgctacgg cgctcacctg gatgaacttc tcacaggcgc
    14281 tgccgcgcag tcagtgggcg caggccgcct ggtcgccgga tattgacgtc atcgagcaga
    14341 tgatttttca ctacagcttg ttgccgcgtc tggcgatttc gctgctggtg ggcgcgggcc
    14401 tggggctggt gggcgtgctg tttcagcaag tactgcgtaa cccgctggcg gaaccgacga
    14461 cgttgggcgt tgcaacaggc gcgcaactgg ggattaccat caccacgctc tgggcgctcc
    14521 ctggcgcgat ggcgagccag tttgctgcgc tggcaggggc ttgtgttgtt ggcttaattg
    14581 tctttggcgt cgcgtggggg aaacggctgt cgccggtaac gctgattctc gcggggttgg
    14641 tagtgagcct ttattgcggc gcaatcaatc agttactggt tatcttccat catgaccagt
    14701 tgcaaagcat gtttctgtgg agcactggaa cgctgacgca aaccgactgg ggcggtgttg
    14761 agcgtttatg gccgcagttg ctgggcggtg tgatgctgac gttgctgcta cttcgtccgt
    14821 taaccctgat ggggcttgat gatggcgtgg cgcgcaatct cgggctggcc ttgtcgcttg
    14881 cgcgtctggc ggcgctgtcg ctggcgattg tcatcagtgc gctgctggtg aacgctgtgg
    14941 ggattatcgg ctttatcggg ttgttcgcgc cgctgctggc aaaaatgctg ggggcgcggc
    15001 gtctgctgcc acgactgatg ctggcgtcgt tgattggtgc gctgatcctc tggctttccg
    15061 atcaaatcat cctctggctg actcgcgtgt ggatggaagt gtccaccggt tcggtcactg
    15121 cgttgatcgg tgcgccgctg ctactgtggc tgttgccgcg tttacgcagc attagcgcgc
    15181 cggatatgaa ggtcaacgat cgtgtcgcgg ctgaacgcca acatgtgctg gcgtttgccc
    15241 tcgcgggcgg cgtgctgctg ttgatggctg tggtggtggc gctgtcgttt ggtcgtgatg
    15301 cgcacggctg gacgtgggcg agcggggcgt tgctcgagga tttaatgccc tggcgctggc
    15361 cgcgaattat ggcggcgctg tttgcgggcg tcatgctggc ggtggcgggc tgtattattc
    15421 agcgactgac cggaaacccg atggcaagcc cggaagtgct ggggattagc tccggcgcgg
    15481 cgtttggcgt ggtgttgatg ctgtttctgg tgccgggtaa tgcctttggc tggctgttac
    15541 ctgcaggcag tctcggcgcg gcggtgacgc tgttgatcat tatgatcgcc gccggccgcg
    15601 gtggattttc cccacaccgt atgttactgg cggggatggc gttaagcacc gcgttcacca
    15661 tgcttttgat gatgttgcag gcaagtggtg acccgcgaat ggcgcaagtg ctgacctgga
    15721 tttccggttc gacctacaac gcgaccgatg cgcaggtctg gcgcaccgga attgtgatgg
    15781 tgattttgct ggcgattacc ccgctgtgcc gccgctggct gaccatttta ccgctgggtg
    15841 gtgataccgc ccgagccgta ggaatggcgc tgacgccgac gcgaattgcg ctgctgctgt
    15901 tagcggcttg cctgacggcg accgcgacga tgactattgg accgttgagt tttgttggtt
    15961 taatggcacc gcatattgcg cggatgatgg gttttcgacg gacgatgcca cacatcgtaa
    16021 tttcggcgct ggtgggtggt ttactactgg tgttcgctga ctggtgtggg cggatggtgc
    16081 tgtttccgtt ccagatcccg gcggggctgc tgtcgacctt tatcggcgcg ccatatttta
    16141 tctatttgct gagaaagcag agccgttaat ttttccatat aacggccctg tacgcctgga
    16201 cggataaagc gtttacgcga caacggatgc ccgatgcgac gctggcgcgt cttatcgggc
    16261 ctacaaacgg ccccgaatcg taggtcggat aaggcgttca cgccgcatcc gacaaaccat
    16321 gctggatcac aacttcgcaa acacccgacg tgcagcatcg atggtgttat tgatatcttc
    16381 catgctgtgc gccacggaca taaagcccgc ttcaaacgct gacggtgcca ggtaaacacc
    16441 ttcgtccagc atcatatgga agaaacgctt aaagcgttcc acgtcacagg ccatcacatc
    16501 ctgatagcac gtcacggact cggcgtcggt aaagaaaata ccgaacatgc cgccaacgtg
    16561 gttaacgacc agcggaattc cggcttcttc tgccgcttcc agcagacctt ctgccagacg
    16621 tgatgtcagc tcatccaacg tttcgtgaac gcccggctgc gcgacttcat tcagacaggc
    16681 gaaacccgct gccatcgcaa ttgggttacc ggaaagcgta cccgcctgat agaccggacc
    16741 cgtcggggcc agcgcatcca ttacatcacg acgaccaccg aatgcgccta ccggcattcc
    16801 accgccgatg attttgccca ggcaggtgag atccggttcc acaccgtaat aatcctgtgc
    16861 gccagccagt gccacgcgga agccggtcat tacttcatcg atgatcagca atgcgccaaa
    16921 ttcgtcgcac agcgcacgca gacctggcag gaactctggc agcggtggaa cgcagttcat
    16981 attgcctgcc accggctcga cgataataca ggcaatctct tgcgggtatt gctcaaacgc
    17041 ggcgcgtaca gaagccagat cgttataagt acaggttaag gtatgtttgg cgaaatctgc
    17101 cggaacgccc ggcgagtttg gctggcctaa cgtgagtgcg ccagaaccgg ctttcaccag
    17161 caggcagtca gcgtgaccgt ggtaacaacc ttcaaattta ataattttgt cgcgaccggt
    17221 aaaaccacgg gccaggcgga tggcgctcat cgtcgcctcg gtgccggagt tcaccatgcg
    17281 caccatatcc atggtcggga ccagttcggt caccagttgc gccattttca cttccatttc
    17341 ggttggtgca ccaaagctta aaccacgctc ggcggcttca atcacggcat tgcggattgc
    17401 cggatggtta tggcccagca ccatcggccc ccaggaaccg acataatcga tataggcttt
    17461 gccatcaaca tcgtacagat aagcgccgtc cgctttttcg ataaacagtg gagtgccgcc
    17521 cacgccagta aaggcgcgaa caggggagtt cacaccgcca gggatcagct cgcgcgctgc
    17581 gctgtaaaga ttttcagact tactcatgga gggttcctga ttcgtagaaa aagtgaatgg
    17641 ctgctattct atgttattca taacaagtta aatgctcgtc aaacatcagg ctgcttgtac
    17701 tggtgcaatc ctgatttctt agagtataaa agttttgtgc atttgaaaca ttacgctttg
    17761 caaaggattt tcatggaacg tgcgagtaaa atgccgtcat cttatttgta tgaccaataa
    17821 gtgatcattg gatgaaaact gatactccct ctttagaaac accgcaggcc gcgcgcctgc
    17881 gacgcagaca actgattcgc caacttcttg agcgcgataa aaccccgtta gccattttgt
    17941 ttatggcggc agtcgtcggc acgcttgtcg ggctggcagc ggttgctttt gacaaaggtg
    18001 tcgcctggtt gcagaaccaa cgtatggggg cgctggtaca tactgctgat aattatccgc
    18061 tactgttaac cgtcgctttt ctctgttcgg cggtgctggc gatgttcggc tactttttgg
    18121 tgcgcaaata cgcgccggaa gcaggtggtt cggggatccc ggaaattgaa ggggcgctgg
    18181 aagatcaacg tcccgttcgc tggtggcgtg tattgccggt gaagttcttt ggcgggctgg
    18241 ggacactcgg cggaggcatg gtgttggggc gcgaagggcc aaccgtgcag atcggcggta
    18301 acattggccg tatggtgctt gatattttcc gcctgaaagg tgacgaagct cgccatacgc
    18361 tgctggcaac cggtgctgct gcggggctgg ctgcggcctt taacgcgccg ctggcgggta
    18421 ttttgtttat tatcgaagag atgcgtccgc agtttcgcta tacgttaatt tcgattaaag
    18481 cggtatttat tggtgtcatt atgtcgacca ttatgtaccg gatttttaat catgaagttg
    18541 cgttgattga cgtcggtaaa ctttctgatg cgccgcttaa tacgctgtgg ctttatctga
    18601 tcctcggtat tatttttggc attttcggcc ctatttttaa taaatgggtg ctggggatgc
    18661 aggatttgct gcaccgtgtg cacggcggca atattaccaa atgggtgctg atgggcggtg
    18721 cgattggcgg tctgtgtgga ttgctggggt ttgtggcacc agcaacgtcg ggcggcggtt
    18781 ttaacctgat tcctatcgct accgcgggga atttcagcat gggaatgctg gtgtttatct
    18841 tcgtcgcgcg ggtcattacc accttactct gcttctcttc cggcgcgccg ggcggtattt
    18901 ttgccccgat gctggcgctg ggtactgtgc tgggaaccgc tttcggaatg gttgccgttg
    18961 agctgtttcc gcaatatcac cttgaggcgg ggacgtttgc tattgccgga atgggggcgt
    19021 tactggcggc atctattcgc gcgccgttaa cggggatcat tctggttctg gagatgaccg
    19081 ataactacca gctcattttg ccaatgatta ttaccggtct tggcgcaaca ctattagcgc
    19141 aatttaccgg cgggaaaccg ctatactcgg cgattcttgc gcgcacgctg gcaaaacagg
    19201 aagctgagca actagcgcga agcaaggccg catcagccag cgagaatact tgaacgaaat
    19261 accagggtat tagataatgg cgattattat tgggttagaa tttgcccaat tgccgatgtc
    19321 gtttggagca aaatatgagt gatgacgtag cactgccgct ggagtttacc gacgcagcag
    19381 ccaacaaagt taaaagcctg atcgctgacg aagataaccc gaatctgaaa ttacgcgtgt
    19441 atatcaccgg tggcggttgc agcggcttcc agtatggttt cacctttgat gatcaggtga
    19501 acgaaggcga tatgaccatc gaaaaacagg gcgttggcct ggtggttgat ccgatgagcc
    19561 tgcaatatct ggtcggcggt tccgttgact ataccgaagg actggaaggt tctcgtttca
    19621 tcgtgaccaa cccgaacgcg aaaagcacct gcggttgcgg ttcttccttt agcatctaat
    19681 ctgttgttca tgtgccggat aaactatccg gcacatcttc tctcttcaac gcccattctc
    19741 atccagcgca aacgtcggta gcttaagatg ccaacgaata gccgccagcc gaatcaatag
    19801 cgtcacgacc atgcccatca tactggctgt ttccagtggt acggaaaatg tgtaataagc
    19861 cgtagcgtgg acaataccgc cgataataca ggcagttgcg tagatttctg tacgtaaaat
    19921 catggggatt tcgcgggcca gaacatcacg aatgatcccg ccgccaacgc cagtaatgac
    19981 gcccatacaa accgcgatta acggaccggc ttccgcatta aaggctttat tcacgccaat
    20041 gccgacaaac accgccagac caacggcgtc cagcaccggc aacatccatt ttggtaagcg
    20101 tcttggctgg cgcaccagca cgatggtcag catgctggtg accattgcaa cgaccagatc
    20161 ggtgggatct ttcacccaaa ataccgggcc gtgatccagc gccatgtcgc gaattgtccc
    20221 gccgcctaca gcggtaacca cgcccagtac cagaacacca aaagggtcca tacgcaattt
    20281 tccggctaac aaaacgccgg agatggcaaa taccgctgtg ccgactatat ccagccaata
    20341 gacgagcatt gttcaatccc cactgagcac ctgaaaaggt ctggcgctaa tctacctgtg
    20401 aaagcgcatt acagagctgt tgtgcagcga ggataatacg tgggcttgca cgttcaaacc
    20461 agtcactcgt gagaggaata acgggaattt tgagctgttc accccagtat tgtttgattt
    20521 taggaatttg gtccggtccg cctgtaatga caatcgcctg tggcgagcgt gctaacacct
    20581 gttcgcggct aacttgcggc cagggaaccc ggctgtcttt aaagatgttt tctccgccac
    20641 aaacttcgag tacctggttt tgaatcgact cttttccact ggtaaatggc ggattaatgc
    20701 cgaattgcag aaaaacacgt tttttaggtt tatcagcata ttgcgctttc aattgcgcgt
    20761 actgatccag cagggattgc gcggcttgtt cggccttatc cggttgcgga ctccaggggg
    20821 ccagttgacg taacgcattg gcaatttgtt caatgcttgt cgcatcgacc cacatcactt
    20881 ttattcccag cgaagccagc tggtcaacct gccgctcggc attacctcca cgccaggcaa
    20941 tcaccagatc gggtttcagc gcgacaatgc gttccagatt catcccctgc caggtggaaa
    21001 cctgctcaat cttttgcgct tgtggaggat agtcggaata gctgctgacc ccaaccggcg
    21061 tgatcccggc ggcaaaggca agttcagtgt tggcgggaga aagcgtgatg acgcgcggcg
    21121 cggcgttgag ccacagtggc gcgagaaaag acagggcgac cagcgccctg aacagtgact
    21181 tagccatgtg ccagtttctg caccagtgac tcaaccatca ggctggactg tttagcggca
    21241 acagccagga actcatcgaa gctaagatga gactgttgat cggccacgtc ggagatggcg
    21301 cgtactacga caaacgggac gttgaaattg tggcagacat gggcgattgc cgtcgcttcc
    21361 atctctacag caatggcctg tgggaagttg tggcggattt tcgccagacc aacagaaccg
    21421 ttgatgaaag cgtcgccgct aacaatcagg ccacgtacag cgttaagatt cagttcggca
    21481 atgcaggcct cagcggcagc gatcagttta tcgtcagctt taaagcctgc cggacagcct
    21541 ggtaactgac cgtattcata accaaatgcc gtgacatccg cgtcgtgata acgtgcttcg
    21601 tccgagacaa cgatatcgcc cactttcaac gttggtgcca ggccaccggc agaaccggtg
    21661 ttaataatca catctggctt gcagtgttcc aacagcagag tggcacccag cgccgcagcg
    21721 actttaccga tgcccgattt cagaagcgca acctcggttc cattcagttg gccggtatag
    21781 atttcgcaac cgccgagact gatagtttga cggttttcga ttttgtcacg cagcagcgta
    21841 acttcttctt ccattgcacc aatgatgccg attttcatag atttactcgc gataagcccg
    21901 atttgaaggc atagtttacc atgcgcttac ggggaagcgt atttctcacg cgggagagga
    21961 catggcacag attgatttcc gaaaaaaaat aaactggcat cgtcgttacc gttcaccgca
    22021 gggcgttaaa accgaacatg agatcctgcg gatcttcgag agcgatcgcg ggcgtatcat
    22081 caactctccg gcaattcgtc gtctgcaaca aaagacccag gtttttccac tggagcgcaa
    22141 tgccgccgtg cgcacgcgtc ttacccactc gatggaagtc cagcaggtgg gacgctacat
    22201 cgccaaagaa attttaagcc gtctgaaaga gcttaaattg ctggaagcat acggcctgga
    22261 tgaactgacc ggcccctttg aaagcattgt tgagatgtca tgcctgatgc acgatatcgg
    22321 caatccgccg tttggtcatt ttggcgaagc agcgataaat gactggtttc gccaacgttt
    22381 gcacccggaa gatgccgaaa gccagcctct gactgacgat cgctgcagcg tggcggcact
    22441 acgtttacgg gacggggaag aaccgcttaa cgagctgcgg cgcaagattc gtcaggactt
    22501 atgtcatttt gaggggaatg cacaaggcat tcgtctggtg catacattga tgcggatgaa
    22561 tctcacctgg gcacaggttg gcggtatttt aaaatatacc cgtccggcgt ggtggcgtgg
    22621 cgaaacgcct gagacacatc actatttaat gaaaaagccg ggttattatc tttctgaaga
    22681 agcctatatt gcgcggttgc gtaaagaact taatttggcg ctttacagtc gttttccatt
    22741 aacgtggatt atggaagctg ccgacgacat ctcctattgt gtggcagacc ttgaagatgc
    22801 ggtagagaaa agaatattta ccgttgagca gctttatcat catttgcacg aagcgtgggg
    22861 ccagcatgag aaaggttcgc tcttttcgct agtggttgaa aatgcctggg aaaaatcacg
    22921 ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac gagtaaacac
    22981 cctaaataaa ctggtaccct acgcggcaca acgatttatt gataatctgc ctgcgatttt
    23041 cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg atcttcttaa
    23101 gctatataaa aatgtcgctg taaaacatgt gtttagccat ccagatgtcg agcagcttga
    23161 attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt tattaagcct
    23221 gtcgttatca gactttactg aactggtaga aaaagaacgg gtgaaacgtt tccctattga
    23281 atcgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg aggctgtcag
    23341 taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattacc gttgccgcct
    23401 gctgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat accgacgtct
    23461 gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt ttaccttttg
    23521 cagaaacttt agttcggaac ttcaggctat aaaacgaatc tgaagaacac agcaattttg
    23581 cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac tgagtgcact
    23641 ggctctgagt ttaggtttgg cgttatctcc gctctctgca acggcggctg agacttcttc
    23701 agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg tgatgccttc
    23761 agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta tgccgcgtaa
    23821 tttccagcag ttcttcggtg atgattctcc gttctgccag gaaggttctc cgttccagag
    23881 ctctccgttc tgccagggtg gccagggcgg taatggtggc ggccagcaac agaaattcat
    23941 ggcgctgggt tccggcgtca tcattgatgc cgataaaggc tatgtcgtca ccaacaacca
    24001 cgttgttgat aacgcgacgg tcattaaagt tcaactgagc gatggccgta agttcgacgc
    24061 gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg atccaaatcc agaacccgaa
    24121 aaacctgacc gcaattaaga tggcggattc tgatgcactg cgcgtgggtg attacaccgt
    24181 agcgattggt aacccgtttg gtctgggcga gacggtaact tccgggattg tctctgcgct
    24241 ggggcgtagc ggcctgaatg ccgaaaacta cgaaaacttc atccagaccg atgcagcgat
    24301 caaccgtggt aactccggtg gtgcgctggt taacctgaac ggcgaactga tcggtatcaa
    24361 caccgcgatc ctcgcaccgg acggcggcaa catcggtatc ggttttgcta tcccgagtaa
    24421 catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac gcggtgagct
    24481 gggtattatg gggactgagc tgaactccga actggcgaaa gcgatgaaag ttgacgccca
    24541 gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag cgggcattaa
    24601 agcgggtgat gtgatcacct cactgaacgg taagccgatc agcagctttg ccgcactgcg
    24661 tgctcaggtg ggtactatgc cggtaggcag caaactgacc ctgggcttac tgcgcgacgg
    24721 taagcaggtt aacgtgaacc tggaactgca gcagagcagc cagaatcagg ttgattccag
    24781 ctccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag atcagggcgt
    24841 ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga agaaaggtga
    24901 tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc gtaaagttct
    24961 cgacagcaaa ccgtctgtgc tggcactcaa cattcagcgc ggcgacagca ccatctacct
    25021 gttaatgcag taatctccct caaccccttc ctgaaaacgg gaaggggttc tccttacaat
    25081 ctgtgaactt caccacaact ccatacatct tcatcatcct ttaggcattt cacaatgccg
    25141 tacgttacgt acttccttat gctaagccgt gcataacgga ggacttatgg ctggctggca
    25201 tcttgatacc aaaatggcgc aggatatcgt ggcacgtacc atgcgcatca tcgataccaa
    25261 tatcaacgta atggatgccc gtgggcgaat tatcggcagc ggcgatcgtg agcgtattgg
    25321 tgaattgcac gaaggtgcat tgctggtact ttcacaggga cgagtcgtcg atatcgatga
    25381 cgcggtagca cgtcatctgc acggtgtgcg gcaggggatt aatctaccgt tacggctgga
    25441 aggtgaaatt gtcggcgtaa ttggcctgac aggtgaacca gagaatctgc gtaaatatgg
    25501 cgaactggtc tgcatgacgg ctgaaatgat gctggaacag tcgcggttga tgcacttgtt
    25561 ggcgcaggat agccgtttgc gggaagaact ggtgatgaac ctgattcagg cagaggagaa
    25621 tactcccgca cttactgaat gggcgcaacg gctggggatc gatctcaatc aaccgcgagt
    25681 ggtggctatt gttgaggtcg acagcggtca gcttggcgtg gacagcgcaa tggcggagtt
    25741 acaacaactg caaaacgcgc tgactacgcc cgagcgtaat aatctggtgg cgattgtctc
    25801 gctaaccgaa atggtggtgt tgaaaccggc gttgaactct tttgggcgct gggatgcaga
    25861 agatcatcgt aagcgagttg aacaactgat tacccgcatg aaagagtacg gccagctgcg
    25921 ttttcgcgtt tcactgggca actattttac cggtcctggc agtattgccc gatcctatcg
    25981 tacggcgaaa acgacgatgg tggtgggtaa acagcggatg ccagaaagtc gctgctattt
    26041 ttatcaggat ctgatgttac ctgtgttact cgacagtttg cgtggcgact ggcaggccaa
    26101 cgaactggcg cgaccgctgg cgcggctgaa aacgatggac aataacggct tgctgcgacg
    26161 aacgctggcg gcgtggtttc gccacaatgt gcaaccgctg gcaacgtcaa aggcgttgtt
    26221 tattcatcgt aataccctgg agtatcggct taatcgtata tcggaactga ccgggcttga
    26281 tttgggcaat tttgatgaca ggttgctgct gtatgtggcg ttacaactgg atgaagagcg
    26341 gtaggttatg cgttaaggtt gaggcggcgc tggctcattc ccctcagccc gaagggtaga
    26401 ggggaatgcc cgaattaacc gtcagttcgg gattatttac gcgttagttt ttccagatcg
    26461 gcttcaatct cgctgatctt attggttacg actgactcca ggtgacgtaa gtcgtcgagg
    26521 atcttacgct taagatcaac ttcgctgcgg tcacgctggc agatttgatc aagctcatca
    26581 atgatatacc gtagattcgg gctgatttcc tggacttctt tataaccctg acccacacca
    26641 tcagcgacga ccgttttacg ctgacgcgga tatttaaact taacgctctt ggcgaaaaac
    26701 tcgcctttgt ctttctggaa atagattttc agaatatcgt tgttggcttc ctgccggagg
    26761 ctgtaacgat caatttcttc aggattggta atacccagac ttttcagatt gtcgtacata
    26821 gcgttacctc aaaatgagtc agtaaattgt gcttatttta gcatttggcc tggcccgccc
    26881 ggctcgactg tgatcgcaga aagccttttt cttccagact tctatcatgg cgtaaaaatc
    26941 aaaaaattac ctgctttatt ctggtgataa aattcacgat ctacaccagt tccagcatcg
    27001 ttacttccgg gcggcagttc agacgcaaac catacaaact gcccacgcca cgggttgtgt
    27061 agatgtgtct ttcgccaaag gcatttaatc cggcgacgta acgtttatct tcgacagggg
    27121 caaaaggttc accaaccaac ggtacgcgca gttgcccgcc gtgagtatgg ccgcacagca
    27181 tcagatccca gggttcatca cgcatgactt ctttgctgtc gggattatgc gccagcacca
    27241 gtcttggcag attggcttcg ctggcgggag gcggtttgca ttgtccggcc cataagtcac
    27301 cagtgcctac cagttcgaat tgcctgttcg gcgtggcgat caccgtagcc tggttaaaca
    27361 acaccgtgat gcccgctgat ttcaacgtct cgccaattaa gtgatttttt tctgtaccaa
    27421 cagggcgatc gtggttgccg aagcaggcaa acgtcggcgc acattcggca aggggggaga
    27481 gtacgtcact aaacgccgaa aaattcagcg acatatcaaa taatacgtaa tcgccgccca
    27541 gtaatatcaa atcgggcttt tgttctatgc caagagcaat cgcgtcagaa atcaggctta
    27601 aaggaacaaa acgagagtaa tggagatcgg ccagaaaaag aattttgaat ggtgctgcgt
    27661 tgtctttaaa aaaggcgagg cggtgacgga ttaattcaaa ccagcctggc tcacagtaat
    27721 gcatataacc gaaacctgag ctcgtggcta tcgtcgcggc agtagcctgc aaaaatcggc
    27781 ggcgtgaaat catcgctcat ccctgcaaaa aaatcgggca gcgtcgtgct gcccgtgtgc
    27841 atacttttag tcgatggtac gcagcagttc gttaatgccg actttgccgc gagttttcgc
    27901 gtcaactttc ttaacgataa ccgcacagta gaggctgtat ttgccatctt ttgacggcag
    27961 attacctgaa acaaccacag accccgccgg aacgcgaccg tagtggattt cgccggtttc
    28021 acggtcgtaa atacgggtgc tctgaccaat gtatacgccc atggaaatga cggaaccttc
    28081 ttcgacaatc accccttcaa ccacttcaga gcgcgcgccg atgaagcaat tatcttcaat
    28141 gatggttggg ttagcctgca gcggttccag cacgccgccg atgcccacgc caccggaaag
    28201 gtggacgttt ttaccaatct gcgcacaaga accgacggtc gcccaggtat caaccatggt
    28261 gccttcatca acatatgcgc cgatgttgac gtaagacggc atcagcacgg tgttacgggc
    28321 aataaacgca ccctgacgta ccgccgctgg tggcacaacg cggaagcctt ctttctggaa
    28381 acgtgcttcg tcgtagtcgg cgaatttcat cggcactttg tcgaagtagc ggctttctgc
    28441 cccttcgatc acctgattat cattaatacg gaaagagagc agcaccgctt ttttcaacca
    28501 ctgatgcgtc acccactgac cgtcaatttt ttccgctaca cgcagtgcgc cggaatccag
    28561 cagggcgatc acctgattta ccgcttcgcg ggtaacggtg tctgcattgg ctggcgtgat
    28621 ctcggcacgg cgttcaaaag cggtttcaat aatgttctgt aactgctgca ttgttaaact
    28681 cttttcatat cagtaaacac atcacccttt atcgtttgga ttgagggcct ctgtcaaccg
    28741 ctgatgcact tcctgctgca actcgttatt aagcgcacgc cggtcagcgg tggcaattat
    28801 gaataaatct tctactcgct cgccaatggt tgtaattcgg gcaccatgaa gcgaaattcc
    28861 cagatcggca aaaattttcc cgactcgcgc cagcagtcca ggttggtcga gggcgatcag
    28921 ttcgaggaac gatttgcggt cggtatgggt cggcaaaaac gttacttcgg tttcaacagt
    28981 aaaatggcgt aatttggcgg gttggcgacg gggctgcggt ggctgccagc tactttgcgt
    29041 cagtacttgc tccagaccaa accgaataac ctcatgacga tctgcggaca gcgggttgcc
    29101 atcgggttcc agcacgataa aggtatccat cgccataccg tcgcgagtgg tgaaaatttg
    29161 tgcgtcgtga acacttaaat tgcggcggtc taattcggca cagacggcgg caaacagata
    29221 agggcggtcc gggctccaga taaaaatctc ggtgcctcca cgcgtagcct gcgggctaag
    29281 caataccagc ggtttgctta aatcatgctg taataaatgg cgggcatgcc aggccagttg
    29341 atttgggcta tggcggacaa aatagttagc acgacagcgt gaccaaattt ggtgcagcgc
    29401 ctcttcgtcg atgttatcca tgcgcagtag tgccagtgcc tggagttggt gatggcgaac
    29461 ccgttcgcgc atatccggcg tgttttgcat tccgcgtcgt agctgctttt cggtggcaaa
    29521 gtagagctca cgcaacagac tttgcttcca gctattccac agcgtttcgt tggtggcgca
    29581 aatgtcagcc acagtcaggc ataccagata gcgcagacga ttttccgttt gcacttcttc
    29641 ggcaaactgc ttgatgactt ccgggtcctg aatatcgcgg cgttgggcgg tcaccgacat
    29701 caacaggtgc tggcgaacca gccacgccac cagttgcgtt tcgcgcgagt taagcccgtg
    29761 gagttcggca aaatgcacta catcctgagc accgagaatg gagtggtcgc cgccgcgtcc
    29821 tttggcgata tcgtgaaaca gcgcggcgat gaaaatcagc tcagttgacg gtaggcgcgg
    29881 ccagacgtcc acacacaacg gatggcgctg gcgcgtttct tcactggcaa aactctccag
    29941 tttcagcatc acgcggatag tatgttcatc caccgtgtag gcgtggaaca gatcaaactg
    30001 catctgcccg acgatatgtg accattgcgg catatacgcg ccgagtacgc tatggcgatg
    30061 cattggcaat agcccgcgcc gcaccgctcc ggggtgacgc agaatgctca aaaacagttt
    30121 tcgtgcttcc ggaatattac acagcggttg ttgcagatgg cgccgggcat ggcgtaactg
    30181 gcgcagcgtg gtggagtaaa tgccggtgat cgcactgttg cgcaccatgg tgtagaacat
    30241 acgtaagata gcttccggct ggcgcataaa tagtgtttca tcacgcaggt cgattagcgt
    30301 accgcgtagc tgaaactcat cgtcgattgg acgtggtttt tcgtcggcgg gaagggcgag
    30361 gatggcttca tcgaacagtt gcagcagcat ctggttgagt tcactgacgc ggcgtgtcac
    30421 gcggaagtaa tccttcatca tccgctcgac cggttcgtta ccttcgccac tgtaattcag
    30481 acgctgggcg acgctaagct ggcgatcgaa taacaggcga ttatcgtaac ggctgacgac
    30541 cagatgcagg gcaaagcgaa tacgccacaa tatatgcaga cattcgttta attccgcccg
    30601 ctccgctgag gttaagaagc caaacccaac catttcatcc agcgatgttg cgccaaaatg
    30661 acggcgggcc acccattgca gagtgtggat atcgcgcaag ccgccagggc tacttttgat
    30721 gtctggttca agattgtagc tggtgccgtg gtaacgctga tggcgctggt tctgttcttc
    30781 aactttcgcc gcgtagaact tgtcggaagg ccagaatcct tcgctgaaaa tatgtttttg
    30841 cagttcaaga aatagcgcaa catcgccaat taataagcgg gattcgatca aattggtggc
    30901 gacggttaaa tccgataacc cttccagaat gcactcttca agcgtgcgca cactatgacc
    30961 gacttccagc tttacatccc agagcagcgt taacagctcg cccacttttt gcgcctgatc
    31021 gtccgggagc tttttacggc ttaaaatcag taaatcgatg tcagaaagtg gatgcagctc
    31081 gccacgaccg tagccaccga cggcgaccaa tgccaggtcg gcaatctggc tgaatcccgc
    31141 ttcaatccat aatcgttgca ggagctggtc gataaactcg gtgcgcgcct caatcaactg
    31201 ttctgcagag atcccattgt caaaggcatc acccagccaa cgctggaaag tatcgatatg
    31261 ggcttttatc ccaccgacgg ttaattcatc acggggccag acgcatggat tttgcggttg
    31321 accgggcagg gtggggagag cggtgtttgc gtactgttct ggaagggtat tcattgtgcg
    31381 ccacccataa gattaaatta tcgcattaaa aaagccggca ttcgccggct tcatcttatt
    31441 cgtcgtgcga gattatcgcc gggatggtgt catccttgcg tagcgtcaga atttcgcagc
    31501 cgttatcagt caccacaata gtatgctcat attgtgcaga caagctgcga tctttggttt
    31561 ttaccgtcca gccatctttc atggtgcgga tctctttttt acccgcgttg accattggct
    31621 cgatggtgaa cgtcatccca ggtttcagta cgacgttggt ttcacgggag tcatagtgca
    31681 gcacctgcgg ttcttcatgg aagccgcgac caataccgtg tccgcaatat tcacgaacga
    31741 cggagaagcc ttctgcttcg acaaatttct gaatcgccgc accgatttcg cgcagattaa
    31801 tgcctggttt taccatgcgt agcgccaggt acaggctttc ttgcgtgatg cggcacagac
    31861 gttcgcccat gatggtcggc ttaccgacga taaacatttt cgaggtatcg ccgtggaaac
    31921 catctttgat tacggtgaca tcaatgttaa cgatatcgcc atctttcagc agcttagcat
    31981 cgtccgggat tccgtggcac accacttcat taatagaaat gcaaacggat ttcggataac
    32041 cgtgatagcc gaggcaggca gaaaccgcgt gttgttcatt aacaatgtaa tcattacaga
    32101 tgcgatccag ctcgccggtg ctgacgcccg gtttaacata tggttcgatc atctccagca
    32161 cttcggcagc cagtcggcca gcgacgcgca ttttttcgat atcttctggg gtcttgattg
    32221 agatagccat taattctgtc catcagcgtc ggtgataccg acaatatata tgtaagtgcc
    32281 gtcaatggta tcacacccgg gcaaattgag aatcattctg aatttcgcca aacgtgccac
    32341 tgaacgtttt ctataataga aaattcaacg tctgatgctg tacacagcgc caacaattat
    32401 tggtgtccat gacgtatttg tggtataaag cgcgccggac ttccgatcca tttcgtatac
    32461 acagactgga cggaagcgac aatctcactt tgtgtaacaa cacacacgta tcggcacata
    32521 ttccggggtg ccctttgggg tcggtaatat ggggtacgtg gaggcataac cccaactttt
    32581 atatagaggt tttaatcatg gcaactgttt ccatgcgcga catgctcaag gctggtgttc
    32641 acttcggtca ccagacccgt tactggaacc cgaaaatgaa gccgttcatc ttcggtgcgc
    32701 gtaacaaagt tcacatcatc aaccttgaga aaactgtacc gatgttcaac gaagctctgg
    32761 ctgaactgaa caagattgct tctcgcaaag gtaaaatcct tttcgttggt actaaacgcg
    32821 ctgcaagcga agcggtgaaa gacgctgctc tgagctgcga ccagttcttc gtgaaccatc
    32881 gctggctggg cggtatgctg actaactgga aaaccgttcg tcagtccatc aaacgtctga
    32941 aagacctgga aactcagtct caggacggta ctttcgaaaa gctgaccaag aaagaagcgc
    33001 tgatgcgcac tcgtgagctg gagaaactgg aaaacagcct gggcggtatc aaagacatgg
    33061 gcggtctgcc ggacgctctg tttgtaatcg atgctgacca cgaacacatt gctatcaaag
    33121 aagcaaacaa cctgggtatt ccggtatttg ctatcgttga taccaactct gatccggacg
    33181 gtgttgactt cgttatcccg ggtaacgacg acgcaatccg tgctgtgacc ctgtacctgg
    33241 gcgctgttgc tgcaaccgta cgtgaaggcc gttctcagga tctggcttcc caggcggaag
    33301 aaagcttcgt agaagctgag taataaggct tgataactcc cccaaaatag ttcgagttgc
    33361 agaaaggcgg caagctcgag aattcccggg agcttacatc agtaagtgac cgggatgagc
    33421 gagcgaagat aacgcatctg cggcgcgaaa tatgaagggg gagagccctt atagaccagg
    33481 tagtacacgt ttggttaggg ggcctgcata tggccccctt tttcactttt atatctgtgc
    33541 ggtttaatgc cgggcagatc acatctccga ggattttaga atggctgaaa ttaccgcatc
    33601 cctggtaaaa gagctgcgtg agcgtactgg cgcaggcatg atggattgca aaaaagcact
    33661 gactgaagct aacggcgaca tcgagctggc aatcgaaaac atgcgtaagt ccggtgctat
    33721 taaagcagcg aaaaaagcag gcaacgttgc tgctgacggc gtgatcaaaa ccaaaatcga
    33781 cggcaactac ggcatcattc tggaagttaa ctgccagact gacttcgttg caaaagacgc
    33841 tggtttccag gcgttcgcag acaaagttct ggacgcagct gttgctggca aaatcactga
    33901 cgttgaagtt ctgaaagcac agttcgaaga agaacgtgtt gcgctggtag cgaaaattgg
    33961 tgaaaacatc aacattcgcc gcgttgctgc gctggaaggc gacgttctgg gttcttatca
    34021 gcacggtgcg cgtatcggcg ttctggttgc tgctaaaggc gctgacgaag agctggttaa
    34081 acacatcgct atgcacgttg ctgcaagcaa gccggaattc atcaaaccag aagacgtatc
    34141 cgctgaagtg gtagaaaaag aataccaggt acagctggat atcgcgatgc agtctggtaa
    34201 gccgaaagaa atcgcagaga aaatggttga aggccgcatg aagaaattca ccggcgaagt
    34261 ttctctgact ggtcagccgt tcgttatgga accaagcaaa accgttggtc agctgctgaa
    34321 agagcataac gctgaagtga ctggcttcat ccgcttcgaa gtgggtgaag gcatcgagaa
    34381 agttgagact gactttgcag cagaagttgc tgcgatgtcc aagcagtctt aattatcgaa
    34441 aaggagccgc ctgagggcgg cttctttttg cgcccatctt gtaaattcag ctaaccctta
    34501 tggggctgcg ctgaaaagcg acgtaaaatg tcgccggtat taattcattt caatcgttga
    34561 cagtctcagg aaagaaacat ggctaccaat gcaaaacccg tctataaacg cattctgctt
    34621 aagttgagtg gcgaagctct gcagggcact gaaggcttcg gtattgatgc aagcatactg
    34681 gatcgcatgg ctcaggaaat caaagaactg gttgaactgg gtattcaggt tggtgtggtg
    34741 attggtgggg gtaacctgtt ccgtggcgct ggtctggcga aagcgggtat gaaccgcgtt
    34801 gtgggcgacc acatggggat gctggcgacc gtaatgaacg gcctggcaat gcgtgatgca
    34861 ctgcaccgcg cctatgtgaa cgctcgtctg atgtccgcta ttccattgaa tggcgtgtgc
    34921 gacagctaca gctgggcaga agctatcagc ctgttgcgca acaaccgtgt ggtgatcctc
    34981 tccgccggta caggtaaccc gttctttacc accgactcag cagcttgcct gcgtggtatc
    35041 gaaattgaag ccgatgtggt gctgaaagca accaaagttg acggcgtgtt taccgctgat
    35101 ccggcgaaag atccaaccgc aaccatgtac gagcaactga cttacagcga agtgctggaa
    35161 aaagagctga aagtcatgga cctggcggcc ttcacgctgg ctcgtgacca taaattaccg
    35221 attcgtgttt tcaacatgaa caaaccgggt gcgctgcgcc gtgtggtaat gggtgaaaaa
    35281 gaagggactt taatcacgga ataattcccg tgatggataa ataagggtaa gattccgcgt
    35341 aagtatcgcg ggggcgtaag tctggttata aggcgttatt gttgcaggca gtttggtcac
    35401 ggccagcgcg cagcaaccgg agcgtacaaa agtacgtgag gatggcgagc actgctcggg
    35461 gccaaaatgg caaataaaat agcctaataa tccagacgat tacccgtaat atgtttaatc
    35521 agggctatac ttagcacact tccactgtgt gtgactgtct ggtctgactg agacaagttt
    35581 tcaaggattc gtaacgtgat tagcgatatc agaaaagatg ctgaagtacg catggacaaa
    35641 tgcgtagaag cgttcaaaac ccaaatcagc aaaatacgca cgggtcgtgc ttctcccagc
    35701 ctgctggatg gcattgtcgt ggaatattac ggcacgccga cgccgctgcg tcagctggca
    35761 agcgtaacgg tagaagattc ccgtactctg aaaatcaacg tgtttgatcg ttcaatgtct
    35821 ccggccgttg aaaaagcgat tatggcgtcc gatctcggcc tgaacccgaa ctctgcgggt
    35881 agcgacatcc gtgttccgct gccgccgctg acggaagaac gtcgtaaaga tctgaccaaa
    35941 atcgttcgtg gtgaagcaga acaagcgcgt gttgcagtac gtaacgtgcg tcgtgacgcg
    36001 aacgacaaag tgaaagcact gttgaaagat aaagagatca gcgaagacga cgatcgccgt
    36061 tctcaggacg atgtacagaa actgactgat gctgcaatca agaaaattga agcggcgctg
    36121 gcagacaaag aagcagaact gatgcagttc tgatttcttg aacgacaaaa acgccgctca
    36181 gtagatcctt gcggatcggc tggcggcgtt ttgcttttta ttctgtctca actctggatg
    36241 tttcatgaag caactcacca ttctgggctc gaccggctcg attggttgca gcacgctgga
    36301 cgtggtgcgc cataatcccg aacacttccg cgtagttgcg ctggtggcag gcaaaaatgt
    36361 cactcgcatg gtagaacagt gcctggaatt ctctccccgc tatgccgtaa tggacgatga
    36421 agcgagtgcg aaacttctta aaacgatgct acagcaacag ggtagccgca ccgaagtctt
    36481 aagtgggcaa caagccgctt gcgatatggc agcgcttgag gatgttgatc aggtgatggc
    36541 agccattgtt ggcgctgctg ggctgttacc tacgcttgct gcgatccgcg cgggtaaaac
    36601 cattttgctg gccaataaag aatcactggt tacctgcgga cgtctgttta tggacgccgt
    36661 aaagcagagc aaagcgcaat tgttaccggt cgatagcgaa cataacgcca tttttcagag
    36721 tttaccgcaa cctatccagc ataatctggg atacgctgac cttgagcaaa atggcgtggt
    36781 gtccatttta cttaccgggt ctggtggccc tttccgtgag acgccattgc gcgatttggc
    36841 aacaatgacg ccggatcaag cctgccgtca tccgaactgg tcgatggggc gtaaaatttc
    36901 tgtcgattcg gctaccatga tgaacaaagg tctggaatac attgaagcgc gttggctgtt
    36961 taacgccagc gccagccaga tggaagtgct gattcacccg cagtcagtga ttcactcaat
    37021 ggtgcgctat caggacggca gtgttctggc gcagctgggg gaaccggata tgcgtacgcc
    37081 aattgcccac accatggcat ggccgaatcg cgtgaactct ggcgtgaagc cgctcgattt
    37141 ttgcaaacta agtgcgttga catttgccgc accggattat gatcgttatc catgcctgaa
    37201 actggcgatg gaggcgttcg aacaaggtca ggcagcgacg acagcattga atgccgcaaa
    37261 cgaaatcacc gttgctgctt ttcttgcgca acaaatccgc tttacggata tcgctgcgtt
    37321 gaatttatcg gtactggaaa aaatggatat gcgcgaacca caatgtgtgg acgatgtgtt
    37381 atctgttgat gcgaacgcgc gtgaagtcgc cagaaaagag gtgatgcgtc tcgcaagctg
    37441 aggataatcc ggctacagag agtcgcgcta tttgttagcg tagggcttca gtgatatagt
    37501 ctgcgccatc tgatcgtaag tagttggctt tataaggtca ggtatgccgt ggttttacac
    37561 ggcttttttt tgtataggct tcagtattcc tgagtaccgt aaaccctgtc agggaataaa
    37621 aaacgcgtga tgttgtctgc tattcaacca cttagcgaaa aattgccagc gcatggctgc
    37681 cgtcatgttg cgatcattat ggacggcaat ggtcgctggg caaaaaagca agggaagatt
    37741 cgagcctttg ggcataaagc cggggcaaaa tccgtccgcc gggctgtctc ttttgcggcc
    37801 aacaacggta ttgaggcgtt aacgctgtat gcctttagta gtgaaaactg gaaccgacca
    37861 gcgcaggaag tcagtgcgtt aatggaactg tttgtgtggg cgctcgatag cgaagtaaaa
    37921 agtctgcacc gacataacgt gcgtctgcgt attattggcg ataccagtcg ctttaactcg
    37981 cgtttgcaag aacgtattcg taaatctgaa gcgctaacag ccgggaatac cggtctgacg
    38041 ctgaatattg cggcgaacta cggtggacgt tgggatatag tccagggagt caggcaactg
    38101 gctgaaaagg tgcagcaagg aaacctgcaa ccagatcaga tagatgaaga gatgctaaac
    38161 cagcatgtct gtatgcatga actggcccct gtagatttag taattaggac tgggggggag
    38221 catcgcatta gtaacttttt gctttggcaa attgcctatg ccgaacttta ctttacagat
    38281 gttctctggc ccgatttcga tgaacaagac tttgaagggg cgttaaatgc ctttgctaat
    38341 cgagagcgtc gtttcggcgg caccgagccc ggtgatgaaa cagcctgatg ggggtcgctt
    38401 ttgctgaagt atcgcctgat atctgctttt gtgttaatac ccgtcgtcat cgcggcgttg
    38461 tttctgttgc cgccggtggg gttcgccatc gtaacgctgg tggtctgcat gctggcagcg
    38521 tgggaatggg gacagcttag cggttttacc actcgttcgc agcgagtatg gttggcggtg
    38581 ttatgcgggt tattgttggc gctgatgctt tttctgttgc cggaatatca ccgaaatatt
    38641 catcaaccgc tggttgaaat ctcactttgg gcttcgctgg gttggtggat tgtcgcgcta
    38701 ttgctggtgc tgttttaccc aggttccgca gcaatctggc gtaactctaa aacattgcgc
    38761 cttatttttg gcgtgctaac cattgttccc ttcttctggg gcatgctggc gttacgggcc
    38821 tggcactatg acgagaatca ttacagtggc gcaatatggc tgctctatgt catgatcctg
    38881 gtatggggcg ctgactctgg cgcatatatg tttggcaaat tgtttggtaa acataagctg
    38941 gcaccgaagg tttctccggg taaaacctgg caaggcttta taggtggact cgcgaccgca
    39001 gcagtaatct catggggtta tggcatgtgg gcgaatctcg acgtcgctcc cgtcacctta
    39061 ctcatttgct ctattgtcgc agcgttagcc tcagtgctcg gcgatctgac cgagagtatg
    39121 tttaagcgtg aagcaggaat taaggacagc ggtcatttaa ttccaggaca cggtggtatt
    39181 ttagatcgta ttgatagcct gacggctgcg gtaccggtct ttgcttgctt gttgttactg
    39241 gtattcagga cgctttaacg gaaggtaata tgctgagttt tctctgggat ttggcttcgt
    39301 tcatcgttgc actgggtgta cttatcaccg tgcatgaatt tggtcatttc tgggttgccc
    39361 ggcgttgtgg tgttcgcgtt gagcgtttct caatagggtt tggtaaggcg ctctggcggc
    39421 gaactgataa gctcggcacc gaatatgtta tcgccctgat cccgctgggc ggttatgtca
    39481 aaatgctgga tgagcgcgca gaaccggtcg ttccggaact ccgccaccat gccttcaata
    39541 ataaatctgt cggccaacga gcggcgatta ttgccgcagg tccggttgca aacttcattt
    39601 ttgctatctt tgcctactgg ctggttttta ttattggtgt gcctggcgta cgtccggtgg
    39661 ttggcgaaat agcagccaat tcgatagctg cggaagcaca aattgcacca ggtacggaac
    39721 taaaagccgt agatggtatc gaaacgcctg attgggatgc cgtgcgtttg cagttggtcg
    39781 ataaaattgg cgatgaaagc accaccatta cagtagcgcc atttggcagc gaccaacggc
    39841 gggatgtaaa gctcgattta cgtcactggg cgtttgagcc tgataaagaa gatccggtat
    39901 cttcgctggg gattcgtcct cgtgggccgc aaattgaacc tgtactggaa aatgtgcaac
    39961 caaactcggc ggcaagcaag gcaggtttgc aagcaggcga caggatcgtt aaagtcgatg
    40021 gtcagccctt aacgcagtgg gtgacctttg tgatgcttgt ccgggataac ccgggtaaat
    40081 ccttagcgtt agaaatcgaa aggcagggaa gtcccttgtc tttgacatta atcccggaga
    40141 gtaaaccggg taatggtaaa gcgattggtt ttgtcggtat tgagccgaaa gtcattcctt
    40201 tgccagatga gtataaagtt gtacgccagt atgggccgtt caacgccatt gtcgaagcca
    40261 cggacaaaac gtggcagctg atgaagctga cggtcagtat gctgggaaaa ttgatcaccg
    40321 gtgatgtgaa actgaacaac ctcagtgggc cgatctctat cgccaagggg gctgggatga
    40381 cagcggaact cggggttgtt tattacctgc cgtttcttgc gcttattagc gtgaacttag
    40441 ggataattaa cctgtttccg ttgcccgtac ttgacggggg gcatctgctg ttccttgcga
    40501 tcgaaaagat caagggcgga ccggtatccg agcgggttca agacttttgt tatcgcattg
    40561 gctcgatttt gctggtgctg ttaatggggc ttgcactttt caatgatttc tctcggttat
    40621 gagagttagt taggaagaac gcataataac gatggcgatg aaaaagttgc tcatagcgtc
    40681 gctgctgttt agcagcgcca ccgtatacgg tgctgaaggg ttcgtagtga aagatattca
    40741 tttcgaaggc cttcagcgtg tcgccgttgg tgcggccctc ctcagtatgc cggtgcgcac
    40801 aggcgacacg gttaatgatg aagatatcag taataccatt cgcgctctgt ttgctaccgg
    40861 caactttgag gatgttcgcg tccttcgtga tggtgatacc cttctggttc aggtaaaaga
    40921 acgtccgacc attgccagca ttactttctc cggtaacaaa tcggtgaaag atgacatgct
    40981 gaagcaaaac ctcgaggctt ctggtgtgcg tgtgggcgaa tcccttgatc gcaccaccat
    41041 tgccgatatc gagaaaggtc tggaagactt ctactacagc gtcggtaaat atagcgccag
    41101 cgttaaagct gtcgtgaccc cgctgccgcg caaccgtgtt gacctaaaac tggtgttcca
    41161 ggaaggtgtg tcagctgaaa tccagcaaat taacattgtt ggtaaccatg ctttcaccac
    41221 cgacgaactg atctctcatt tccaactgcg tgacgaagtg ccgtggtgga acgtggtagg
    41281 cgatcgtaaa taccagaaac agaaactggc gggcgacctt gaaaccctgc gcagctacta
    41341 tctggatcgc ggttatgccc gtttcaacat cgactctacc caggtcagtc tgacgccaga
    41401 taaaaaaggt atttacgtca cggtgaacat caccgaaggc gatcagtaca agctttctgg
    41461 cgttgaagtg agcggcaatc ttgccgggca ctccgctgaa attgagcagc tgactaagat
    41521 cgagccgggc gaactgtata acggcaccaa agtgaccaag atggaagatg acattaaaaa
    41581 gcttctcggt cgctatggtt atgcctatcc gcgcgtacag tcgatgcctg aaattaacga
    41641 tgccgacaaa accgttaaat tacgcgtgaa cgttgatgcg ggtaaccgtt tctacgtgcg
    41701 taagatccgt tttgaaggta acgatacctc gaaagatgcc gtcctgcgtc gcgaaatgcg
    41761 tcagatggaa ggtgcatggc tggggagcga tctggtcgat cagggtaagg agcgtctgaa
    41821 tcgtctgggc ttctttgaaa ctgtcgatac cgatacccaa cgtgttccgg gtagcccgga
    41881 ccaggttgat gtagtctaca aggtaaaaga gcgcaacacc ggtagcttca actttggtat
    41941 tggttacggt actgaaagtg gcgtgagctt ccaggctggt gtgcaacagg ataactggtt
    42001 aggtacaggt tatgctgttg gtatcaacgg gaccaaaaac gattaccaga cctatgctga
    42061 actgtcggta accaacccgt acttcaccgt agatggcgta agcctcggtg gtcgtctctt
    42121 ctataatgac ttccaggcag atgatgccga cctgtccgac tataccaaca agagttatgg
    42181 tacagacgtg acgttgggct tcccgattaa cgaatataac tcgctgcgtg caggtctggg
    42241 ttatgtacat aactccctgt ccaacatgca gcctcaggtt gcgatgtggc gttatctgta
    42301 ctctatgggt gaacatccga gcacctctga tcaggataac agcttcaaaa cggacgactt
    42361 cacgttcaac tatggttgga cctataacaa gcttgaccgt ggttacttcc cgacagatgg
    42421 ttcacgtgtc aacctgaccg gtaaagtgac cattcctgga tcggataacg aatactacaa
    42481 agtgacgtta gacacggcga cttatgtgcc gatcgatgac gatcacaaat gggttgttct
    42541 ggggcgtacc cgctggggtt atggtgatgg tttaggcggc aaagagatgc cgttctacga
    42601 gaacttctat gccggtggtt ccagcaccgt gcgtggcttc cagtccaata ccattggtcc
    42661 gaaagcagtt tacttcccgc atcaggccag taattatgat ccggactatg attacgaatg
    42721 tgcgactcag gacggcgcga aagacctgtg taaatcggat gatgctgtag gcggtaacgc
    42781 tatggcggtt gccagcctcg agttcatcac cccgacgccg ttcattagcg acaagtatgc
    42841 taactcggtt cgtacttcct tcttctggga tatgggtacc gtttgggata caaactggga
    42901 ttccagccaa tattctggat atccggacta tagtgatcca agcaatatcc gtatgtctgc
    42961 gggtatcgca ttacaatgga tgtccccatt ggggccgttg gtgttctcct acgcccagcc
    43021 gttcaaaaag tacgatggag acaaggcaga acagttccag tttaacatcg gtaaaacctg
    43081 gtaagtgttc tccacaaagg aatgtagtgg tagtgtagcg atgactttag gcgatcaata
    43141 taagatcgcc gggccacgca aagaactgca ccctccggtg caaatgggat ggtaaggagt
    43201 ttattgtgaa aaagtggtta ttagctgcag gtctcggttt agcactggca acttctgctc
    43261 aggcggctga caaaattgca atcgtcaata tgggcagcct gttccagcag gtagcgcaga
    43321 aaaccggtgt ttctaacacg ctggaaaatg agttcaaagg ccgtgccagc gaactgcagc
    43381 gtatggaaac cgatctgcag gctaaaatga aaaagctgca gtcaatgaaa gcgggcagcg
    43441 atcgcactaa gctggaaaaa gacgtgatgg ctcagcgcca gacttttgct cagaaagcgc
    43501 aggcttttga gcaggatcgc gcacgtcgtt ccaacgaaga acgcggcaaa ctggttactc
    43561 gtatccagac tgctgtgaaa tccgttgcca acagccagga tatcgatctg gttgttgatg
    43621 caaacgccgt tgcttacaac agcagcgatg taaaagacat cactgccgac gtactgaaac
    43681 aggttaaata agtaatgcct tcaattcgac tggctgattt agcgcagcag ttggatgcag
    43741 aactacacgg tgatggcgat atcgtcatca ccggcgttgc gtccatgcaa tctgcacaaa
    43801 caggtcacat tacgttcatg gttaacccaa aataccgtga gcatttaggc ttgtgccagg
    43861 cgtccgcggt tgtcatgacc caggacgatc ttcctttcgc gaaaagtgcc gcgctggtag
    43921 tgaagaatcc ctacctgact tacgcgcgca tggcgcaaat tttagatacc acgccgcagc
    43981 ccgcgcagaa cattgcaccc agtgcggtga tcgacgcgac ggcgaagctg ggtaacaacg
    44041 tatcgattgg cgctaacgcg gtgattgagt ccggcgttga actgggcgat aacgtgatta
    44101 tcggtgccgg ttgcttcgta ggtaaaaaca gcaaaatcgg tgcaggttcg cgtctctggg
    44161 cgaacgtaac catttaccat gagatccaga tcggtcagaa ttgcctgatc cagtccggaa
    44221 cagtggtagg cgcagacggc tttggttatg ccaacgatcg tggtaactgg gtgaagatcc
    44281 cacagattgg tcgcgtaatt attggcgatc gcgtggagat cggtgcctgc acaaccatcg
    44341 atcgcggcgc gctggatgac actattattg gcaatggcgt gatcattgat aaccagtgcc
    44401 agattgcaca taacgtcgtg attggcgaca atacggcggt tgccggtggc gtcattatgg
    44461 cgggcagcct gaaaattggt cgttactgca tgatcggcgg agccagcgta atcaacgggc
    44521 atatggaaat atgcgacaaa gtgacggtta cgggcatggg tatggtgatg cgtcccatca
    44581 ctgaaccagg cgtctattcc tcaggcattc cgctgcaacc caacaaagtc tggcgcaaaa
    44641 ccgctgcact ggtgatgaac attgatgaca tgagcaagcg tctgaaatcg cttgagcgca
    44701 aggttaatca acaagactaa cgttccatct tttgttcgcc aaactttacg gcctgtctca
    44761 ttcttacgat tgcggcaggc cgtgttatta ttgtcgtttc ttatattttg acaggaagag
    44821 tatcttgact actaacactc atactctgca gattgaagag attttagaac ttctgccgca
    44881 ccgtttccct ttcttactgg tggatcgcgt gctggatttt gaagaaggtc gttttctgcg
    44941 cgcagtaaaa aatgtctctg tcaatgagcc attcttccag ggccatttcc ctggaaaacc
    45001 gattttcccg ggtgtgctga ttctggaagc aatggcacag gcaacaggta ttctggcgtt
    45061 taaaagcgta ggaaaactgg aaccgggtga gctgtactat ttcgctggta ttgacgaagc
    45121 gcgcttcaag cgcccggtcg tgcctggcga tcaaatgatc atggaagtca ctttcgaaaa
    45181 aacgcgccgc ggcctgaccc gttttaaagg ggttgctctg gtcgacggta aagtagtttg
    45241 cgaagcaacg atgatgtgtg ctcgtagccg ggaggcctga tacgtgattg ataaatccgc
    45301 ctttgtgcat ccaaccgcca ttgtggaaga gggcgcgtcg attggcgcga acgcacacat
    45361 tggtcctttt tgtatcgttg gaccccatgt cgaaattggt gagggtaccg tactgaaatc
    45421 tcacgttgtc gtgaatggcc atactaaaat tggccgcgat aatgagattt atcagttcgc
    45481 ctccatcggt gaagttaacc aggatctgaa atatgctggc gaaccgaccc gtgtggaaat
    45541 cggcgatcgt aaccgcattc gcgaaagcgt caccattcat cgtggcacag tccagggcgg
    45601 tggattgacg aaggtgggca gcgacaactt actgatgatc aacgcgcaca ttgcgcacga
    45661 ttgtacggta ggtaaccgct gtattctcgc caacaacgca acgctggcgg gtcacgtatc
    45721 ggtagacgac ttcgcgatca tcggcggcat gaccgcagtc catcagttct gcatcattgg
    45781 tgcgcatgtg atggttggcg gttgctccgg tgtggcgcag gacgtccctc cttatgtcat
    45841 tgcgcagggt aaccacgcaa cgccgttcgg tgtcaatatc gaagggctga agcgccgcgg
    45901 attcagccgc gaggcgatta ccgctatccg caatgcgtat aagctgattt atcgcagcgg
    45961 taaaacgctc gatgaagtga aaccggaaat tgctgaactg gcggaaacgt atccggaagt
    46021 gaaagctttt accgatttct ttgcacgctc aacgcgcggt ctgattcgtt aatgactgaa
    46081 cagcgtccat taacgattgc cctggtcgcc ggagaaacct ccggcgatat cctgggggcc
    46141 ggtttaatcc gcgctctgaa agaacgtgtg cccaacgccc gctttgttgg cgttgccggg
    46201 ccacgaatgc aggctgaagg ctgcgaagcc tggtacgaaa tggaagaact ggcggtgatg
    46261 ggcattgttg aagtgctcgg tcgtctgcgt cgcttactgc atattcgtgc cgatctgaca
    46321 aagcgttttg gcgaactgaa gccagatgtt tttgttggta ttgatgcgcc cgacttcaat
    46381 attactcttg aaggtaacct caaaaagcag ggtatcaaaa ccattcatta cgtcagtcca
    46441 tccgtctggg cgtggcgaca gaaacgcgtt ttcaaaatag gcagagccac cgatctggtg
    46501 ctcgcatttc tgcctttcga aaaagcgttt tatgacaaat acaatgtacc gtgccgcttt
    46561 atcggtcata ccatggctga tgccatgccg ttagatccag ataaaaatgg tgcccgtgat
    46621 gtgctgggga tcccttacga tgcccactgt ctggcattgt tgccgggcag ccgtggtgca
    46681 gaagtcgaaa tgcttagtgc cgatttcctg aaaactgccc agcttttgcg ccagacatat
    46741 ccggatctcg aaatcgtggt gccgctggtg aatgccaaac gccgcgagca gtttgaacgc
    46801 atcaaagctg aagtcgcgcc agacctgtcg gttcatttgc tggatggaat gggccgtgag
    46861 gcgatggtcg ccagcgatgc ggcactactg gcgtcgggga cggcagccct ggagtgtatg
    46921 ctggcgaaat gcccgatggt ggtgggatat cgcatgaagc cttttacctt ctggttggcg
    46981 aagcggctgg tgaaaactga ttatgtctcg ctgccaaatc tgctggcggg cagagagtta
    47041 gtcaaagagt tattgcagga agagtgtgag ccgcaaaaac tggctgcggc gctgttaccg
    47101 ctgttggcga acgggaaaac cagccacgcg atgcacgata ccttccgtga actgcatcag
    47161 cagatccgct gcaatgccga tgagcaggcg gcacaagccg ttctggagtt agcacaatga
    47221 tcgaatttgt ttatccgcac acgcacctgg ttacgggtgt ggatgaagtc ggacgcgggc
    47281 cgttagttgg cgcggtcgtc accgctgcgg tgattctcga cccggcgcgc ccgattgccg
    47341 ggctgaatga ttccaaaaag ctgagcgaaa aacgtcgtct ggcgctctgt gaagagatca
    47401 aagagaaagc gttgagctgg agtctgggcc gcgcggaacc ctacgaaatc gacgagctga
    47461 acattcttca tgcgaccatg ctggcgatgc agcgagccgt cgctgggctg catattgcgc
    47521 cggaatatgt gttgattgat ggtaaccgct gcccgaaatt accgatgcct tcgatggcag
    47581 tggtgaaagg cgatagccgc gtaccggaaa tcagtgccgc gtctatcctg gcgaaagtga
    47641 cgcgcgacgc cgaaatggcg gcgctggata ttgttttccc gcaatatggt tttgcccaac
    47701 ataaagggta tccaaccgct tttcatctgg aaaaactggc tgaatatggc gcgactgaac
    47761 atcatcgtcg cagctttggg cctgtcaaac gcgcactggg acttgcgtcc tgattcttgt
    47821 gtcgagatta agtaaaccgg aatctgaaga tgtctgaacc acgtttcgta cacctgcggg
    47881 tgcacagcga ctactcgatg atcgatggcc tggccaaaac cgcaccgctg gtaaaaaagg
    47941 cggcggcgtt gggtatgcca gcactggcga tcaccgattt caccaacctt tgcggtctgg
    48001 tgaagttcta cggagcggga catggcgcag ggattaagcc tatcgtcggg gcagatttta
    48061 atgtccagtg cgacctgctg ggtgatgagt taacccacct gacggtactg gcggcgaaca
    48121 ataccggcta tcagaatctg acgttgctga tctcaaaagc gtatcagcgc gggtacggtg
    48181 ccgccgggcc gatcatcgat cgcgactggc ttatcgaatt aaacgaaggg ttgatccttc
    48241 tttccggcgg gcgcatgggc gacgtcggac gcagtctttt gcgtggtaac agcgcgctgg
    48301 tagatgagtg tgtcgcgttt tatgaagaac acttcccgga tcgctatttt ctcgagctga
    48361 tccgcaccgg caggccggat gaagaaagct atttgcacgc ggcggtggaa ctggcggaag
    48421 cgcgcggttt gcccgtcgtg gcgaccaacg acgtgcgctt tatcgacagc agcgactttg
    48481 acgcacacga aatccgcgtc gcgatccacg acggctttac cctcgacgat cctaaacgcc
    48541 cgcgtaacta ttcgccgcag caatatatgc gtagcgaaga ggagatgtgc gagctgtttg
    48601 ccgacatccc agaagccctt gccaacaccg ttgagatcgc caaacgctgt aacgtaactg
    48661 tgcgtcttgg tgaatacttc ctgccgcagt tcccgaccgg ggacatgagc accgaagatt
    48721 atctggtcaa gcgtgcaaaa gagggcctgg aagagcgtct ggccttttta ttccctgacg
    48781 aggaagaacg tgttaagcgc cgcccggaat atgacgaacg tctggagact gaacttcagg
    48841 ttatcaacca gatgggcttc ccgggctact tcctcatcgt tatggaattt atccagtggt
    48901 cgaaagataa cggcgtaccg gtagggccgg gccgtggttc tggtgcaggg tcactggtgg
    48961 cctacgcgct gaaaatcacc gacctcgatc cgctggaatt tgacctgctg ttcgaacgtt
    49021 tccttaaccc ggaacgtgtc tccatgcctg acttcgacgt tgacttctgt atggagaaac
    49081 gcgaccaggt tatcgagcac gtagcggaca tgtacggtcg tgatgcggta tcgcagatta
    49141 tcaccttcgg tacgatggcg gcgaaagcgg tgatccgcga cgtaggccgc gtgctggggc
    49201 atccgtacgg ctttgtcgac cgtatctcga aactgatccc gcccgatcct ggcatgacgc
    49261 tggcgaaagc gtttgaagcc gagccgcagc tgccggaaat ctacgaagcg gatgaagaag
    49321 ttaaggcgct gatcgacatg gcgcgcaaac tggaaggggt cacccgtaac gccggtaagc
    49381 acgccggtgg ggtggttatc gcgccgacca aaattaccga ttttgcgccg ctttattgcg
    49441 atgaagaggg caaacatccg gtcacccagt ttgataaaag cgacgttgaa tacgccggac
    49501 tggtgaagtt cgacttcctt ggtttgcgta cgctcaccat catcaactgg gcgctggaga
    49561 tgatcaacaa gcggcgggcg aagaatggcg agccgccgct ggatatcgcc gcgatcccgc
    49621 tggatgacaa gaaaagcttc gacatgctgc aacgctcgga aaccacggcg gtattccagc
    49681 ttgaatcgcg cggcatgaag gacctgatca agcgtctgca acctgactgc ttcgaagata
    49741 tgatcgcact ggtggcactg ttccgccctg gtccgttgca atcagggatg gtggataact
    49801 ttatcgaccg taaacatggt cgcgaagaga tctcctatcc ggacgtacag tggcagcatg
    49861 aaagcctgaa accggtactg gagccaacct acggcatcat cctgtatcag gaacaggtca
    49921 tgcagattgc gcaggtgctt tctggttata ccctcggtgg cgcggatatg ctgcgtcgtg
    49981 cgatgggtaa gaaaaagccg gaagagatgg ctaagcagcg ttctgtattt gctgaaggtg
    50041 cagaaaagaa cggaatcaac gccgaactgg cgatgaaaat cttcgacctg gtggagaaat
    50101 tcgctggtta cggatttaac aaatcgcact ctgcggccta tgctttggtg tcatatcaaa
    50161 cgttatggct gaaagcgcac tatccggcag agtttatggc ggcggtaatg accgccgata
    50221 tggacaacac cgagaaggtg gtgggcctgg tggatgagtg ctggcggatg gggctgaaaa
    50281 tcctgccacc agatataaac tccggtcttt accatttcca cgtcaacgac gacggcgaaa
    50341 tcgtgtatgg tattggcgcg atcaaagggg tcggtgaagg tccgattgag gccatcatcg
    50401 aagcccgtaa taaaggcggc tacttccgcg aactgtttga tctctgcgcc cgaaccgaca
    50461 ccaaaaagtt aaaccggcgg gtgctggaaa aactgattat gtccggggcg tttgaccgtc
    50521 ttgggccaca ccgcgcggca ctgatgaact cgctgggcga tgcgttaaaa gcggcagatc
    50581 aacacgcgaa agcggaagct atcggtcagg ccgatatgtt cggcgtgctg gccgaagagc
    50641 cggaacaaat tgaacaatcc tacgccagct gccaaccgtg gccggagcag gtggtattag
    50701 atggggaacg tgaaacgtta ggtctgtacc tgacgggaca ccctatcaac cagtatttaa
    50761 aagagattga gcgttatgtc ggaggcgtaa ggctgaaaga catgcacccg acagaacgtg
    50821 gtaaagtcat cacggctgcg gggctcgttg ttgccgcgcg ggttatggtc accaagcgcg
    50881 gcaatcgtat cggtatctgc acgctggatg accgttccgg gcggctggaa gtgatgttgt
    50941 ttactgacgc cctggataaa taccagcaat tgctggaaaa agaccgcata cttatcgtca
    51001 gcggacaggt cagctttgat gacttcagcg gtgggcttaa aatgaccgct cgcgaagtga
    51061 tggatattga cgaagcccgg gaaaaatatg ctcgcgggct tgctatctcg ctgacggaca
    51121 ggcaaattga tgaccagctt ttaaaccgac tccgtcagtc tctggaaccc caccgctctg
    51181 ggacaattcc agtacatctc tactatcaga gggcggatgc acgcgcgcgg ttgcgttttg
    51241 gcgcgacgtg gcgtgtctct ccgagcgatc gtttattaaa cgatctccgt ggcctcattg
    51301 gttcggagca ggtggaactg gagtttgact aatacaggaa tactatgagt ctgaatttcc
    51361 ttgattttga acagccgatt gcagagctgg aagcgaaaat cgattctctg actgcggtta
    51421 gccgtcagga tgagaaactg gatattaaca tcgatgaaga agtgcatcgt ctgcgtgaaa
    51481 aaagcgtaga actgacacgt aaaatcttcg ccgatctcgg tgcatggcag attgcgcaac
    51541 tggcacgcca tccacagcgt ccttataccc tggattacgt tcgcctggca tttgatgaat
    51601 ttgacgaact ggctggcgac cgcgcgtatg cagacgataa agctatcgtc ggtggtatcg
    51661 cccgtctcga tggtcgtccg gtgatgatca ttggtcatca aaaaggtcgt gaaaccaaag
    51721 aaaaaattcg ccgtaacttt ggtatgccag cgccagaagg ttaccgcaaa gcactgcgtc
    51781 tgatgcaaat ggctgaacgc tttaagatgc caatcatcac ctttatcgac accccggggg
    51841 cttatccggg cgtgggcgca gaagagcgcg gtcagtctga agccattgca cgcaacctgc
    51901 gtgaaatgtc tcgccttagc gtaccgacta tttgtaccgt tatcggtgaa ggtggttctg
    51961 gcggcgcgct ggcgattggc gtgggcgata aagtgaatat gctgcaatac agcacctatt
    52021 ccgttatctc gccggaaggt tgtgcgtcca ttctgtggaa gagcgctgat aaagcgccgc
    52081 tggcggctga agcgatgggt atcatcgctc cgcgtctgaa agaactaaaa ctgatcgact
    52141 ccatcatccc agaaccgctg ggtggtgctc accgtaaccc ggaagcgatg gcggcatcgt
    52201 tgaaagcgca actgttggcg gatctggccg atctcgacgt gttaagcact gaagatttaa
    52261 aaaatcgtcg ttatcagcgc ctgatgagct acggttacgc gtaattcgca aaagttctga
    52321 aaaagggtca cttcggtggc ccttttttat cgccacggtt tgagcaggct atgattaagg
    52381 aaggattttc caggaggaac acatgaacat cattgccatt atgggaccgc atggcgtctt
    52441 ttataaagat gagcccatca aagaactgga gtcggcgctg ctggcgcaag gctttcagat
    52501 tatctggcca caaaacagcg ttgatttgct gaaatttatc gaacataacc ctcgaatttg
    52561 cggcgtgatt tttgactggg atgagtacag tctcgattta tgtagcgata tcaatcagct
    52621 taatgaatat ctcccgcttt atgccttcat caacacccac tcgacgatgg atgtcagcgt
    52681 gcaggatatg cggatggcgc tctggttttt tgaatatgcg ctggggcagg cggaagatat
    52741 cgccattcgt atgcgtcagt acaccgacga atatcttgat aacattacac cgccgttcac
    52801 gaaagccttg tttacctacg tcaaagagcg gaagtacacc ttttgtacgc cggggcatat
    52861 gggcggtacc gcctatcaaa aaagcccggt tggctgtctg ttttatgatt ttttcggcgg
    52921 gaataccctc aaggctgatg tctctatttc ggttaccgag cttggttcgt tgctcgacca
    52981 caccgggcca cacctggaag cggaagagta catcgcgcgg acttttggcg cggaacagag
    53041 ttatatcgtt accaacggaa catcgacgtc gaacaaaatt gtgggtatgt acgccgcgcc
    53101 atccggcagt acgctgttga tcgaccgcaa ttgtcataaa tcgctggcgc atctgttgat
    53161 gatgaacgat gtagtgccag tctggctgaa accgacgcgt aatgcgttgg ggattcttgg
    53221 tgggatcccg cgccgtgaat ttactcgcga cagcatcgaa gagaaagtcg ctgccaccac
    53281 gcaggcacaa tggccggttc atgcggtgat taccaactcc acctatgatg gcttgctcta
    53341 caacaccgac tggatcaaac agacgctgga tgtcccgtcg atccacttcg attccgcctg
    53401 ggtgccgtac acccattttc atccgatcta ccaggggaaa agtggtatga gcggcgagcg
    53461 tgttgcggga aaagtgatct tcgaaacgca atcgacccac aaaatgctgg cggcgttatc
    53521 gcaggcttcg ctgatccaca ttaaaggcga gtatgacgaa gaggcgttta acgaagcctt
    53581 tatgatgcat accaccacct cgcccagtta tcccattgtt gcttcggttg agacggcggc
    53641 ggcgatgctg cgtggtaatc cgggcaaacg gctgattaac cgttcggtgg agcgggcgct
    53701 gcattttcgc aaagaggtcc agcggttgcg ggaagagtct gacggttggt ttttcgatat
    53761 ctggcaaccg ccgcaggtgg atgaagccga atgctggccc gttgcgccag gcgaacagtg
    53821 gcacggcttt aacgatgcgg atgccgatca tatgtttctc gatccggtta aagtcactat
    53881 tttgacaccg gggatggacg aacagggcaa tatgagcgag gaggggatcc cggcggcgct
    53941 ggtggcaaaa ttcctcgacg aacgtgggat cgtagtagag aaaaccggcc cttataacct
    54001 gctgtttctc tttagtattg gtatcgataa aaccaaagca atgggattat tgcgtgggtt
    54061 gacggaattt aaacgctctt acgatctcaa cctgcggatc aaaaatatgc tacccgatct
    54121 ctatgctgaa gatcccgatt tctaccgcaa tatgcgtatt caggatctgg cgcaagggat
    54181 ccataagctg attcgtaaac acgatcttcc cggtttgatg ttgcgggcat tcgatacttt
    54241 gccggaaatg atcatgacgc cacatcaggc atggcagaga cagattaaag gtgaagtaga
    54301 aaccattgcg ctggaacaac tggtcggtag agtatcggca aatatgatcc tgccttatcc
    54361 accgggcgta ccgctgttga tgccaggaga aatgctgacc aaagagagcc gcacggtact
    54421 cgattttcta ctgatgcttt gttccgtagg gcaacattac cccggttttg aaacggatat
    54481 tcacggcgcg aaacaggacg aagacggcgt ttaccgcgta cgagtcctaa aaatggcggg
    54541 ataacttgcc agagcggctt ccgggcgagt aacgtgctgt taacaaataa aggagacgtt
    54601 atgctgggtt taaaacaggt tcaccatatt gcgattattg cgacggatta tgcggtgagc
    54661 aaagctttct actgcgatat tcttggtttc acgctgcaaa gcgaagtcta tcgcgaagcg
    54721 cgcgactcgt ggaaagggga tttggcgctt aatgggcaat atgtgattga gcttttctct
    54781 tttccgttcc cgccggagcg ccccagccga ccggaagctt gcggcctgcg tcatctggct
    54841 tttagcgttg atgacatcga tgcggcagtg gcgcaccttg aaagccataa cgtgaagtgt
    54901 gaagccatcc gtgtcgatcc atacacgcaa aaacgcttca ccttctttaa cgatccggac
    54961 gggctgccgt tggaactgta tgagcagtaa ggcttgtcat cgccgcattt gcccggtaac
    55021 gtgccgggca ttgctactgt aaaatcgcac catcatgaca ctcacgctca atagacaact
    55081 tctcacctca cgccagattc tggtggcctt tagcggcggg cttgactcca ccgttctgct
    55141 gcatcagttg gtgcagtggc ggacggaaaa tccgggcgtc actctgcgcg ctattcatgt
    55201 gcatcacggt ttaagtgcca atgccgatgc ctgggttacg cattgcgaaa acgtctgcca
    55261 acagtggcag gtgccgctgg tggtcgaacg cgtacaactt gcgcaagagg ggctggggat
    55321 tgaggcccag gcgcggcagg tgcgttatca ggcatttgcc cgcaccttgt tgcccggtga
    55381 agtgctggtc accgcgcaac atctcgacga tcaatgtgaa acttttctgc tggcgctaaa
    55441 acgcggtagc ggtcctgccg ggctttcggc tatgggggaa gtttcggagt ttgccggaac
    55501 acggcttatc cgcccgttgc tcgcccgcac gcggggggaa ctggagcagt gggcattagc
    55561 gcatggttta cgctggattg aagacgaaag taatcaggac gactcatacg atcgtaactt
    55621 tctgcgcctg cgtgtagtgc cgttattgca gcagcgttgg ccgcattttg ccgaagcaac
    55681 ggctcgcagc gccgcacttt gtgctgaaca agagagcctg ctggatgaat tgctggcaga
    55741 tgatttagcg cactgccaaa cgccgcaggg gacgctgcag attgcgccaa tgctggcgat
    55801 gagtgatgcc cgtcgcgcgg caattatccg ccgctggctg gcagggcaga atgcaccgat
    55861 gccttcccgc gacgcgctgg tgcggatctg gcaggaagtg gcgctggcgc gggaagatgc
    55921 ctccccctgt ttacgctttg gcgcgtttga aatacgccgc tatcaatcgc aactgtggtg
    55981 gattaaatcc gttgccgggc aaagcgaaac cattgtgccg tggcagacgt ggctacaacc
    56041 gctggaatta ccggcggggc tgggaagtgt acagcttact gcgggagtcg atattcgccc
    56101 tccgcgtgca gacgaagcgg tcagcgtgcg tttcaaagcg ccaggattgc tgcatattgt
    56161 cggtcgcaac ggcggacgta agctaaagaa aatctggcaa gagctgggcg tgccgccgtg
    56221 gctacgtgac accacgccac tgctgtttta tgacgaaacg ctgattgcgg cggcaggggt
    56281 atttgtgacg caagaaggtg tggctgaagg tgagaatggc gtaagttttg tctggcagaa
    56341 aacgcttagt taagtgaaag ccggataaga cgcatcaaac gtcgcatccg gcgaaagtca
    56401 atcaggactc gctcaccacc accgtaccga tttccgggtg gctaaagctg gtaattttat
    56461 ccagacgtag ctcacgggtt tcgccagcgg cctcgacgac cagatactcc acatttttgc
    56521 gggagactaa atcgctggct tttgcctgca atttttcgcc atctttcagc tcaagtgtca
    56581 gcattaaatg atgctggcag gcgagctcaa gattatcgta atcatcacaa ttgattggtt
    56641 gatacgtatc attcattgac ataatcgctc accagtaagt ttgccgcagc gtatgctgct
    56701 ttttccctga cagcctcaga aagggcgtcg tcggcagcca tttcattcag cactttcaaa
    56761 acgcagccca gcgcgtccgg aacgtatcct aagtctccgc tggcgatttc cgcgtaccgc
    56821 ttgcgtatta actcacaata tttttccaca tgccctcctg tcagcactct gacttaaccg
    56881 tggatgcaag tctaagccta cgaagataaa ctctgtttcg caaggtgact ataccacact
    56941 catttctgca atatcagcgc cgcaactgca cgtattccgt tacaatggcc tcctgattcg
    57001 aaaggagttt tcttatggcg cttaaagcga caatttataa agcgacggtt aatgtggccg
    57061 atctcgaccg caaccagttt ctcgatgcct ctctgacgct ggcgcgccac ccttcagaaa
    57121 cccaggagcg tatgatgctg cgcttgctgg cgtggctgaa atatgctgat gaacgtctgc
    57181 aatttacccg tggtttgtgt gccgatgatg agccggaagc gtggctgcgt aacgatcacc
    57241 tgggcattga tttgtggatt gagctggggc tgccggatga gcggcggatt aagaaagcct
    57301 gcacccaggc cgcagaagtg gcgctgttta cctataatag tcgggcggcg caaatctggt
    57361 ggcagcaaaa tcagagcaaa tgtgtgcagt ttgccaatct ttccgtctgg tatctggacg
    57421 atgaacaact ggcgaaagta agcgcctttg ccgatcgtac catgacgctg caggcaacga
    57481 ttcaggatgg cgtgatctgg ttatcggacg ataagaataa tctggaagtt aacttaaccg
    57541 cctggcaaca accttcatga ttgtgatttc ccgacatgtt gctattcccg atggtgagct
    57601 tgagatcacc gccattcgtg cgcagggcgc gggcgggcag catgttaata agacctcaac
    57661 ggccattcat ctgcgttttg acattcgggc gtccagcctg ccagagtatt acaaagagcg
    57721 tctgctcgcc gccagccatc atttgatcag cagtgatggc gtgattgtca ttaaggcaca
    57781 ggaataccgc agtcaggaac tgaaccgcga agccgcgctg gcccggctgg tggcagtgat
    57841 taaagattta acaacagaac aaaaagcccg acgacccacg cggcccaccc gtgcatcgaa
    57901 agagcgcagg ctggcatcga aagcacaaaa atcaagcgtg aaagcgttgc gcggcaaagt
    57961 gcgcagcggt cgggaataaa aagaaggaat ggatggtgaa aaaagcgata gtgacagcga
    58021 tggctgtaat cagcctcttt acactgatgg gatgtaataa tcgggccgaa gtcgatacgc
    58081 tttctccggc gcaggctgcc gaactgaaac cgatgccgca aagttggcgc ggcgtgctgc
    58141 cgtgtgctga ttgcgaaggg atcgaaacct ctttgtttct tgaaaaagac ggaacatggg
    58201 tgatgaacga acgttatctg ggcgcgcgtg aagaaccttc ctccttcgct tcctacggta
    58261 catgggcgcg aaccgctgac aagttggtat taaccgacag caaaggtgaa aagtcatatt
    58321 atcgggcgaa aggcgatgcg ctggagatgc tcgatcgtga aggcaatccg attgaatcgc
    58381 agttcaacta tacgctggaa ccggcacaat ccagtttacc tatgacgccg atgaccctgc
    58441 gaggcatgta tttttatatg gctgatgcgg cgaccttcac tgattgcgcg accggaaaac
    58501 gtttcatggt agcgaataac gcagagctgg agcgtggcta cctggctgcg cgcggtcaca
    58561 gtgagaaacc ggtgttactg tcagtagaag gtcactttac gcttgagggt aatccggata
    58621 ccggtgcgcc gactaaagta ttggcacccg atacggcagg gaaattttac cccaaccagg
    58681 attgcagtag tttggggcag taacccgtct tgagacagaa acaaacgcaa aacaggccag
    58741 aaggatatat ttcaacattt tgaatttgca cgttttttgt aggccggata aggcgtttac
    58801 gccgcatccg gcaatggtgc tcaacgcctg atgcgacgct ggcgcgtctt atcatgccta
    58861 caacccccct cataccttaa ggcggataag gcatttactt tatcaccggg tcaaaccaac
    58921 aaagcgaccc gccttgatat aaatcccaag cttcaggtgc ccaacatact gtaactgcgt
    58981 ttctggctta aacgcagaga catcgccagt acgcatatgc atcagatcgg cagggctaat
    59041 ccagcctgat tgcgccagcg taagcggatg cccggctttg gcaaatgcat cagtgacaaa
    59101 ttccgaacaa aaccacgact ttttgtctcc ttcgcccaca ctgcttaact gcgctttcgc
    59161 caggccgctg acgcactgtt ggcgaaaatc ctcggagaac ggattcagtg agcacatctg
    59221 gcgagtcacc ataaagggaa taaattcgac aatgccgcga tagttataac cgctatcttt
    59281 gattttattg gcaaacgcgg tgatatctgt ggcttgttgc ggggtaagat ccgggactcg
    59341 taagacgaaa agcttatcac tatgcttcat cgctttttta agggaaacaa tctggacgcc
    59401 agcgcctgtc gcttctgcaa cgttattatc acccagaaag attgcaacgt gactcacaga
    59461 ggaagtgctg aagacgcgga ttccaaatga ggttacccca aggcttgagg agaacagcaa
    59521 atcgccgggt ttgagatcag gtgccgttat ttcttttatt gattgttcgg tgaaagagct
    59581 ttgatgctgg aatttgactg cccatgtttt cgccccggca tctaccgctg tggctgatga
    59641 gtctggctgg ctgatatcta cggtacaggc cgaaagtaaa agaaagctgg ggagaagcag
    59701 gcggtagtac gcctttggtt tatccatttt atacaatcca tgtaaaaaag ggccctgaaa
    59761 ttcaggaccc tttctggcat cagcctttaa tctgtttcac cagatattcg acgatgtcac
    59821 cagtcttaat taactgtttc tcgccgttgc gacgatattt atattcgata tcgtcgttgt
    59881 cgaggttacg gtcgcccagc acaatagtgt gcggaatacc gatcagttcc atatcagcaa
    59941 acatcacgcc cggacgctct ttgcggtcat ccagcagcac ttcgatacct tgtgcacgca
    60001 gttcgctgta cagtttctca gcaagctcct gtacgcggaa ggatttgtgc atgttcatcg
    60061 gcagaatcgc cacctggaac ggtgcgatcg cgtcaggcca tacgatgccg cgttcgtcgt
    60121 agttctgctc aatcgccgca gccacaacgc gcgttacccc gataccgtag caacccatag
    60181 tcaggatttg gttacggcca tcttcaccct gtacggaggc tttcagtgct tcggagtact
    60241 tggtacccag ctggaagatg tgaccaactt cgataccacg tttgatcagc agcgtaccct
    60301 ggccatccgg gcttggatcg ccagccacca cgttacggat atccgcaact tccggggtag
    60361 cgacatcgcg atcccagttg atgccgaagt agtgtttacc atcgatgtta gcaccagcag
    60421 cgaaatcact catcgccgca acggtacggt caatcaccac cggaatcggc atgtttaccg
    60481 gacccagtga acccggaccg gctttaacca cagcacgaat ttcttcttcg gtcgcgaaag
    60541 tcagcgggct tgcaacctgc ggcagttttt ctgctttaac ttcgttcagc tcgtggtcac
    60601 cgcgcaccag cagcgcaact aacgggaagc tactgccttc aaccgcttta accagcagag
    60661 tcttaaccgt tttctcaatc ggcagattga actgttcaac cagttccgcg atggttttcg
    60721 cgttcggcgt atcaaccagc gtcatttcct gggtagccgc agcgcgcggt tctttcggcg
    60781 cgatagcttc tgccagttca atgttcgctg catagtcaga ggtgtcggag aagaccacat
    60841 cgtcttcacc gctctgcgcc agcacctgga attcgtgaga ggcgctgccg ccgatagaac
    60901 cggtgtcggc ctgtacggcg cggaaatcca gccccatgcg gctgaagatt ttgctgtagg
    60961 ccgcatacat tgcatcgtag gtttcctgca gggattcctg ggaagtatgg aaagagtaag
    61021 catctttcat caggaattcg cgggaacgca tgacgccgaa acgcggacgc acttcgtcgc
    61081 ggaacttggt ctggatctga tagaagttca gcggcagctg tttgtaagag ctaagctcgt
    61141 tacgaatcag gtcagtgata acttcttcat gagttgggcc gagtacgaac ggacgctcgc
    61201 cacggtcaac aaaacgcagc agttccggac cgtactgttc ccaacgacca ctctcttgcc
    61261 acaaatcggc tggctgaacc accggcatcg acacctcgat tgcaccggcg ttgttcatct
    61321 cttcacgcac gatgttttcg acttttttca gaacgcgcac gccggtcggc agccaggtat
    61381 ataacccgga ggccagcttg cggatcatcc cggcgcgcag catcagctga tggctgatca
    61441 cctcggcgtc ggcaggtgtc tccttgagag tggagagcag gtattggcta gtacgcatgt
    61501 tgttacggtt ccagttggaa ggtagaacag gctcaaggcg agcctgggac aaaaaaagtg
    61561 atttagttta ccagtgcaaa agaaatgtca aaagagaagg gcgtgaattt aacgcggttc
    61621 cagcgcaaag acttcaaaac ctgcgtcggt gacgcgccag cgaacgttaa aatcatgtag
    61681 ccagacggca taggttttgc ccgtttcctc acctttacga taggccgggc gcgggtcctg
    61741 cgccagtact tcgcggataa acagcgttaa ctgcggataa cgcttctcca gcgtcaaaag
    61801 ctgcttttcg acctctgcgg taaaactcac cgccatctct gcagctggcg cactttgcgc
    61861 atagctggca ctggcatcgg gaagcgattc ggcaaagggg agatacggtt tgatatccac
    61921 taccggcgta ccatcgacca gatccagact gccgagcttc agaatcacgc tgtctttatg
    61981 gcaaacaacc tctttcagct ctaccagcga catgccaatt gggttagggc ggaaagtaga
    62041 gcgtgttgcg aaaaccccca ttctggcgtt accgccgagg cgcggtggac gcacagtcgg
    62101 acgccagccg ccttccatcg tttgatgaaa gacgaaaagg atccataaat ggctgaacgc
    62161 ttccaggccg cgaacggcgt cggcctggtt gtagggagca atgagatgca gttcgccgtt
    62221 ggcgcttttt accagacctg gctggcgcgg aacggcgaat ttttctttat agggcgagcg
    62281 aataacgcct atttgctcga actggaaact gctcatttcg ccgtaatgtt aagcgcagaa
    62341 ccgatacata cagcctgacg atagcagccc ggcgtaccgc tggtgacttc gcagctatgc
    62401 agtaatacag cattggcttt cattttagag gcgttgattt gcatccgctt acgtgcggtt
    62461 ggaatgctcg gcggagagtc ctgattagag gcctggcaag agtcgccact gacttcaccg
    62521 agatcgcgga acggtttgcc gactaattct tctgcattgg tataaattcg gaccggcgtg
    62581 gcgcgcggcg ctttcggttt tgcaggctcc gctttcggct ggggtgcagt gctttgaacg
    62641 ggttcgacag gggatctgct taacatggaa cagccgctta gcatgagtgc tactaaacag
    62701 atcggtaaag cacgcatagt atttcctcaa tgtatgatca aaacgtcaat attgaatcag
    62761 gagcttgtaa aaatgacaag acgggcaagc gcccgtcctg aatgatatta caaattgtgg
    62821 aaacagccta aaaattacca gcctttaaca gcgccgccgt taaacacttt gtttgctgct
    62881 tcgtaaactt cgtcagactg ataagcctga acgaatttct tcacgttctc ggcgtctttg
    62941 ttatcttcac gcgttacgat caggtttacg tacggggact ctttatcttc gacaaagata
    63001 ccgtctttcg ccggagtcag gccaatctgg ctggcatagg tggtattgat aactgccaga
    63061 gcgatttgcg cgtcgtccag agaacgcggc agctgcggtg cttccagctc aacaattttc
    63121 agatttttgg ggttctcaac aacatcaaga acggtcggca gcaggccaac gccatctttc
    63181 agtttgatca agcccacttt ttgcagcagc agcagtgaac gaccaaggtt agttgggtcg
    63241 tttggcacgg caacctgcga accatcctgc agttcatcca gtgatttgat tttcttggag
    63301 taaccagcaa tcggataaac aaaagtgttg cctactgcga ccagtttgta gccacgatct
    63361 ttcagttgct gatcaaggta cggtttatgc tggaaggcgt tggcgtcgat atcgcctttg
    63421 ctcaatgctt cgtttggcag aacatagtcg ttgaaggtta ccagctcaac gtccaggcca
    63481 tatttgtctt tcgcaacttt ctgcgcgact tctgctacct gctgttcggc accaacaatc
    63541 acgccgactt taatgtggtt tggatctttt tcatcctgac cgcagcctac cagtgccagt
    63601 gatccaatca gggctcccac tgccgcaaag gttttgaatt tgaacgccat accttattcc
    63661 ttcttcaatt atttatgttg tgttgaacgt tacttgcgag tgacagcccg gacgatgcgg
    63721 tcgcctgcga actgaattaa ataaaccaga atgaccagca ataccagtac cgtattcatc
    63781 accgtcgcgt tgtagccgat gtagccatac tgatagccaa tctgacctaa accaccggca
    63841 ccgactgcac cacccatcgc ggaataaccg accagggtaa tcagggtgat agtcgcagca
    63901 ttcaccagac ccggcaatgc ttccggtaac agcactttac ggacgatctg catcggcgtg
    63961 gcacccattg cgcgggaagc ttcaattaat ccggttggga tctccagcag agcgttctcg
    64021 accatacggg caataaacgg tgctgcacca acggttaacg gaacaatcgc tgcctgcaaa
    64081 ccaatcgatg taccgacaat aacgcgggta aacggaatca tccataccag caagataatg
    64141 aacgggatgg aacggaaaat gttcacaatc gcagaaacgg tacgatacag cttcgcgtta
    64201 gcaataattt gccccggacg cgtgacataa agtaggacgc caaccggcag accaatcaca
    64261 aagccaaaaa atccggatac gaaggtcatt gccagcgttt cccatacgcc acgaaccagc
    64321 agccacatca tcggctcaga cataacccag tacctctact tttacatggt gttcctgcag
    64381 ccaggcaatg gcggcttgcg tatcttgttg tgtgccgtgc atttcagtca gcatgatgcc
    64441 gaacttaacg ccaccggcgt aatccatctg cgcgctaata atgttgttgt tgacgttgaa
    64501 acgacgcgcg gtttcagaaa gcagtggggc atcgaccgat tgaccggtaa actccagacg
    64561 caacatcggc acgcagtcag tgaatggctc cgcttgcaga cgttcctggt aatcttccgg
    64621 gatatccaga tgcagggtag actgaataaa cttctgcgcc agcggcgttt tcggatgtga
    64681 gaacacttca cttaccgtgt cctgctcgat cagttcgccg ttgctgatga ccgccacgca
    64741 atcacaaata cgcttcacaa cgtccatttc gtgggtgatc aacagaatcg tcaaacccag
    64801 acggcggttg atgtctttca gcagttcgag aatagaacgt gtcgttgccg ggtccagcgc
    64861 gctggtggct tcatcacaca gcaatacttt gggattgctg gctaatgcac gggcaatcgc
    64921 cacacgttgt ttctgcccac cggaaagatt cgacgggtag ctatcatact tatcgccaag
    64981 accaaccaat gacagcaatt ccgtcacgcg acgtttgatc tcgtctttcg gtgtgttgtc
    65041 cagctccagc ggcagagcca cgttgccaaa aacagtacgc gaagagagca ggttaaaatg
    65101 ctggaaaatc ataccaatct ggcggcgagc tttggtcaac tcagattctg acagcgtggt
    65161 cagctcctgg ccatcgacca gcacgctacc ctcggttggg cgctccagca ggtttacaca
    65221 acgtataagc gtactcttac ccgcgcctga ggcaccgata acgccataaa tttgtccagc
    65281 tggcacatgc aggctgacgt tgttcaacgc ctggatggtg cgggtgccct ggtggaacac
    65341 tttggtgata ttcgaaagtt ttatcattga ttatttatta tcgtcattaa gttagtcgtg
    65401 gcatttcgaa tgcctgaaac gggcaacgcc gtcaatgaaa tggatgttaa ggcatccaga
    65461 cgtctaaatc aatcaggttt atgcgaagag cactttcttg caggtcgaaa catgcgatac
    65521 tagcgtcaca tgccttatta aggagctata aaaggtggcg aagagcgtac ccgcaatttt
    65581 tcttgaccgt gatggcacca ttaatgtcga tcacggctat gtccatgaga tcgacaactt
    65641 tgaatttatc gacggtgtta ttgacgccat gcgcgagcta aaaaaaatgg gctttgcgct
    65701 ggtggtagta accaaccagt ctggcattgc tcgcggtaaa tttaccgaag cacagtttga
    65761 aacgctgacc gagtggatgg actggtcgct ggcggaccga gatgtcgatc tggatggtat
    65821 ctattattgc ccgcatcatc cgcagggtag tgttgaagag tttcgccagg tctgcgactg
    65881 ccgtaaacca catccgggga tgcttttgtc agcacgcgat tatttgcata ttgatatggc
    65941 cgcttcttat atggtgggcg ataaattaga agatatgcag gcagcggctg cggcgaacgt
    66001 gggaacaaaa gtgctggtgc gtacgggtaa acctattacg cctgaagcag aaaacgcggc
    66061 ggattgggtg ttaaatagcc tggcagacct gccgcaagcg ataaaaaagc agcaaaaacc
    66121 ggcataatga ttaaaagatg agcgattgaa ataaaaatgc atttttccac ttgtcttcct
    66181 gagacgactc cctataatgc gcctccatcg acacggcgga tgtgaatcac ttcacacaaa
    66241 cagccggttc ggttgaagag aaaaatcctg aaattcaggg ttgactctga aagaggaaag
    66301 cgtaatatac gccacctcgc gacagtgcgc taaagcgcgt cgcaactgct ctttaacaat
    66361 ttatcagaca atctgtgtgg gcactcgaag atacggattc ttaacgtcga aagacgaaaa
    66421 atgaatacca agtctcaaga gtgaacacgt aattcattac gaagtttaat tctttgagca
    66481 tcaaactttt aaattgaaga gtttgatcat ggctcagatt gaacgctggc ggcaggccta
    66541 acacatgcaa gtcgaacggt aacaggaaac agcttgctgt ttcgctgacg agtggcggac
    66601 gggtgagtaa tgtctgggaa actgcctggt ggagggggat aactactgga aacggtagct
    66661 aataccgcat aacgtcgcaa gaccaaagag ggggaccttc gggcctcttg ccatcggatg
    66721 tgcccagatg ggattagcta gtaggtgggg taacggctca cctaggcgac gatccctagc
    66781 tggtctgaga ggatgaccag ccacactgga actgagacac ggtccagact cctacgggag
    66841 gcagcagtgg ggaatattgc acaatgggcg caagcctgat gcagccatgc cgcgtgtatg
    66901 aagaaggcct tcgggttgta aagtactttc agcggggagg aagggagtaa agttaatacc
    66961 ttt
//
LOCUS       DAPHBJ010000006        63235 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.006, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000006 DAPHBJ010000000
VERSION     DAPHBJ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.006"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            201..731
                     /gene="rppH"
                     /locus_tag="RHZ76_000348"
     CDS             201..731
                     /gene="rppH"
                     /locus_tag="RHZ76_000348"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HDV2811051.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            744..2990
                     /gene="ptsP"
                     /locus_tag="RHZ76_000349"
     CDS             744..2990
                     /gene="ptsP"
                     /locus_tag="RHZ76_000349"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDV2811052.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            3141..4016
                     /gene="lgt"
                     /locus_tag="RHZ76_000350"
     CDS             3141..4016
                     /gene="lgt"
                     /locus_tag="RHZ76_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDV2811053.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            4023..4817
                     /gene="thyA"
                     /locus_tag="RHZ76_000351"
     CDS             4023..4817
                     /gene="thyA"
                     /locus_tag="RHZ76_000351"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDV2811054.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGNTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            5001..5471
                     /locus_tag="RHZ76_000352"
     CDS             5001..5471
                     /locus_tag="RHZ76_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDV2811055.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            5462..6025
                     /locus_tag="RHZ76_000353"
     CDS             5462..6025
                     /locus_tag="RHZ76_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDV2811056.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVNASYSVTGFNL"
     gene            6022..6429
                     /locus_tag="RHZ76_000354"
     CDS             6022..6429
                     /locus_tag="RHZ76_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HDV2811057.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAETDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            6414..6737
                     /locus_tag="RHZ76_000355"
     CDS             6414..6737
                     /locus_tag="RHZ76_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDV2811058.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            6750..10118
                     /gene="recC"
                     /locus_tag="RHZ76_000356"
     CDS             6750..10118
                     /gene="recC"
                     /locus_tag="RHZ76_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HDV2811059.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLLDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            10294..13182
                     /gene="ptrA"
                     /locus_tag="RHZ76_000357"
     CDS             10294..13182
                     /gene="ptrA"
                     /locus_tag="RHZ76_000357"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HDV2811060.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            13175..16717
                     /gene="recB"
                     /locus_tag="RHZ76_000358"
     CDS             13175..16717
                     /gene="recB"
                     /locus_tag="RHZ76_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HDV2811061.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRKEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDVVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQEQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDVSTAELNAKTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVASVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VREKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSARNKQVEMEFYLPISEP
                     LIASQLDTLIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYEHHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            16717..18543
                     /gene="recD"
                     /locus_tag="RHZ76_000359"
     CDS             16717..18543
                     /gene="recD"
                     /locus_tag="RHZ76_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HDV2811062.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(18605..19936)
                     /gene="argA"
                     /locus_tag="RHZ76_000360"
     CDS             complement(18605..19936)
                     /gene="argA"
                     /locus_tag="RHZ76_000360"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HDV2811063.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            20168..21421
                     /gene="amiC"
                     /locus_tag="RHZ76_000361"
     CDS             20168..21421
                     /gene="amiC"
                     /locus_tag="RHZ76_000361"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HDV2811064.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIKDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            complement(21495..21571)
                     /locus_tag="RHZ76_000362"
     tRNA            complement(21495..21571)
                     /locus_tag="RHZ76_000362"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21535..21537),aa:Met,seq:cat)
     gene            21780..22877
                     /gene="mltA"
                     /locus_tag="RHZ76_000363"
     CDS             21780..22877
                     /gene="mltA"
                     /locus_tag="RHZ76_000363"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HDV2811065.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            23116..23922
                     /gene="tcdA"
                     /locus_tag="RHZ76_000364"
     CDS             23116..23922
                     /gene="tcdA"
                     /locus_tag="RHZ76_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HDV2811066.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(23973..24416)
                     /gene="csdE"
                     /locus_tag="RHZ76_000365"
     CDS             complement(23973..24416)
                     /gene="csdE"
                     /locus_tag="RHZ76_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HDV2811067.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(24416..25621)
                     /gene="csdA"
                     /locus_tag="RHZ76_000366"
     CDS             complement(24416..25621)
                     /gene="csdA"
                     /locus_tag="RHZ76_000366"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HDV2811068.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATQQFYS
                     LSAGNVHRSQFAEAQRLTTRYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDLYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            25813..26040
                     /locus_tag="RHZ76_000367"
     CDS             25813..26040
                     /locus_tag="RHZ76_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HDV2811069.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            26391..27308
                     /gene="gcvA"
                     /locus_tag="RHZ76_000368"
     CDS             26391..27308
                     /gene="gcvA"
                     /locus_tag="RHZ76_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HDV2811070.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            27327..27722
                     /locus_tag="RHZ76_000369"
     CDS             27327..27722
                     /locus_tag="RHZ76_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDV2811071.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            27715..28815
                     /gene="rlmM"
                     /locus_tag="RHZ76_000370"
     CDS             27715..28815
                     /gene="rlmM"
                     /locus_tag="RHZ76_000370"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HDV2811072.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNFKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(28859..29590)
                     /gene="fucR"
                     /locus_tag="RHZ76_000371"
     CDS             complement(28859..29590)
                     /gene="fucR"
                     /locus_tag="RHZ76_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HDV2811073.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(29648..30070)
                     /gene="fucU"
                     /locus_tag="RHZ76_000372"
     CDS             complement(29648..30070)
                     /gene="fucU"
                     /locus_tag="RHZ76_000372"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HDV2811074.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(30072..31490)
                     /gene="fucK"
                     /locus_tag="RHZ76_000373"
     CDS             complement(30072..31490)
                     /gene="fucK"
                     /locus_tag="RHZ76_000373"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HDV2811075.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGIGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(31599..33374)
                     /gene="fucI"
                     /locus_tag="RHZ76_000374"
     CDS             complement(31599..33374)
                     /gene="fucI"
                     /locus_tag="RHZ76_000374"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HDV2811076.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(33407..34723)
                     /gene="fucP"
                     /locus_tag="RHZ76_000375"
     CDS             complement(33407..34723)
                     /gene="fucP"
                     /locus_tag="RHZ76_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HDV2811077.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            35270..35917
                     /gene="fucA"
                     /locus_tag="RHZ76_000376"
     CDS             35270..35917
                     /gene="fucA"
                     /locus_tag="RHZ76_000376"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDV2811078.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            35945..37093
                     /gene="fucO"
                     /locus_tag="RHZ76_000377"
     CDS             35945..37093
                     /gene="fucO"
                     /locus_tag="RHZ76_000377"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDV2811079.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALNDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(37148..37903)
                     /gene="xni"
                     /locus_tag="RHZ76_000378"
     CDS             complement(37148..37903)
                     /gene="xni"
                     /locus_tag="RHZ76_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HDV2811080.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(38015..39382)
                     /gene="sdaB"
                     /locus_tag="RHZ76_000379"
     CDS             complement(38015..39382)
                     /gene="sdaB"
                     /locus_tag="RHZ76_000379"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HDV2811081.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(39440..40729)
                     /gene="sdaC"
                     /locus_tag="RHZ76_000380"
     CDS             complement(39440..40729)
                     /gene="sdaC"
                     /locus_tag="RHZ76_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HDV2811082.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(41286..42650)
                     /gene="ppnN"
                     /locus_tag="RHZ76_000381"
                     /gene_synonym="ygdH"
     CDS             complement(41286..42650)
                     /gene="ppnN"
                     /locus_tag="RHZ76_000381"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HDV2811083.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(42762..43610)
                     /gene="queF"
                     /locus_tag="RHZ76_000382"
     CDS             complement(42762..43610)
                     /gene="queF"
                     /locus_tag="RHZ76_000382"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDV2811084.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            43678..44223
                     /gene="syd"
                     /locus_tag="RHZ76_000383"
     CDS             43678..44223
                     /gene="syd"
                     /locus_tag="RHZ76_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HDV2811085.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            44845..45174
                     /locus_tag="RHZ76_000384"
     CDS             44845..45174
                     /locus_tag="RHZ76_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HDV2811086.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            45174..45956
                     /gene="truC"
                     /locus_tag="RHZ76_000385"
     CDS             45174..45956
                     /gene="truC"
                     /locus_tag="RHZ76_000385"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HDV2811087.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            45974..46423
                     /locus_tag="RHZ76_000386"
     CDS             45974..46423
                     /locus_tag="RHZ76_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDV2811088.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            46858..48210
                     /gene="gudP"
                     /locus_tag="RHZ76_000387"
     CDS             46858..48210
                     /gene="gudP"
                     /locus_tag="RHZ76_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HDV2811089.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNKKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            48212..49552
                     /locus_tag="RHZ76_000388"
     CDS             48212..49552
                     /locus_tag="RHZ76_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase-related protein"
                     /protein_id="HDV2811090.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGDVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQREAITVLGYLFYIGDRTKTD
                     LPYVENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTQNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            49573..50913
                     /gene="gudD"
                     /locus_tag="RHZ76_000389"
     CDS             49573..50913
                     /gene="gudD"
                     /locus_tag="RHZ76_000389"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDV2811091.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(51145..53901)
                     /gene="barA"
                     /locus_tag="RHZ76_000390"
     CDS             complement(51145..53901)
                     /gene="barA"
                     /locus_tag="RHZ76_000390"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HDV2811092.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEAVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            53958..55259
                     /gene="rlmD"
                     /locus_tag="RHZ76_000391"
     CDS             53958..55259
                     /gene="rlmD"
                     /locus_tag="RHZ76_000391"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDV2811093.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            55307..57541
                     /locus_tag="RHZ76_000392"
     CDS             55307..57541
                     /locus_tag="RHZ76_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDV2811094.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            57619..57867
                     /gene="mazE"
                     /locus_tag="RHZ76_000393"
     CDS             57619..57867
                     /gene="mazE"
                     /locus_tag="RHZ76_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HDV2811095.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            57867..58202
                     /gene="mazF"
                     /locus_tag="RHZ76_000394"
     CDS             57867..58202
                     /gene="mazF"
                     /locus_tag="RHZ76_000394"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HDV2811096.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            58273..59064
                     /gene="mazG"
                     /locus_tag="RHZ76_000395"
     CDS             58273..59064
                     /gene="mazG"
                     /locus_tag="RHZ76_000395"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDV2811097.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            59287..60924
                     /gene="pyrG"
                     /locus_tag="RHZ76_000396"
     CDS             59287..60924
                     /gene="pyrG"
                     /locus_tag="RHZ76_000396"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HDV2811098.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            61012..62310
                     /gene="eno"
                     /locus_tag="RHZ76_000397"
     CDS             61012..62310
                     /gene="eno"
                     /locus_tag="RHZ76_000397"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDV2811099.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(62370..>63235)
                     /locus_tag="RHZ76_000398"
     CDS             complement(62370..>63235)
                     /locus_tag="RHZ76_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HDV2811100.1"
                     /translation="FILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKELTHK
                     LESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSDRTV
                     RIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAINALNNQLTSQHQYPTN
                     PSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDLFLF
                     SDKIFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSDSGGFSGGGG
                     SSGGGGASGRW"
BASE COUNT        15403 a        15456 c        17054 g        15322 t
ORIGIN      
        1 ttccgtagtt cttcacaact ctatccacag aaaaggtgaa taaaaacggc tatccacccc
       61 ttcctctgtt tataactctg cacataactg tgagttattc aattgttatt agtgtgtaat
      121 gccctgcaga gagtggttta ccgtcttcct ggagtatgaa acaatcattc gtatataaag
      181 ctttattttg aggtagtccg gtgattgatg acgatggcta ccgcccaaac gtaggtatcg
      241 tgatttgtaa tcgccagggg caggtaatgt gggcccggcg atttggtcag cactcctggc
      301 aatttccgca aggcggaatc aaccccggag aatccgcaga gcaggcgatg taccgtgaat
      361 tgtttgaaga agtaggatta agccgcaaag acgttcgaat ccttgcttca acgcgtaact
      421 ggttgcgcta caaattaccg aaacgtttgg tgcgttggga cacgaagccg gtttgtatcg
      481 gccaaaaaca aaaatggttt ctcttgcagc tggtgagcgg cgatgcagaa atcaatatgc
      541 aaaccagcag tacaccagag tttgacggct ggcgatgggt aagttactgg tatccggtca
      601 gacaggtggt gtcatttaaa cgtgatgtct accgtagggt aatgaaagag ttcgcgagtg
      661 tggtgatgtc actgcaggaa aatacgccaa aaccacaaaa cgcatctgct tatcgacgta
      721 aaagaggtta agtcacgcca attatgctca ctcgcctgcg cgaaatagtc gaaaaggtag
      781 ccagcgcacc acgcctgaat gaggcgttaa atattctggt taccgacatc tgtcttgcga
      841 tggataccga ggtctgttcg gtctacctgg ccgatcatga tcgacgttgt tactacctga
      901 tggcgacccg ggggctgaaa aaaccacgcg gtcgcactgt aacgctcgcg tttgatgaag
      961 ggatcgtcgg cctggttggc aggctggcgg aaccgataaa ccttgcagat gcgcaaaagc
     1021 accccagctt caaatacatc ccctccgtaa aagaagaacg tttccgcgcg tttttaggcg
     1081 taccaattat tcaacgtcgc cagttgcttg gtgtactggt ggtacagcaa cgagagttgc
     1141 gccagtatga cgaaagtgaa gaatccttcc tggtgacgct tgccacccag atggcagcta
     1201 ttctttctca gtcgcagttg actgccttgt ttgggcaata tcgccagacg cgaatccgcg
     1261 cattaccggc agcacctggt gtggcgattg ccgaaggctg gcaggatgcc acgttacctt
     1321 taatggaaca ggtgtatcag gcatcaacgc tggatccggc tctggaacgc gaacgactga
     1381 ccggggcgct ggaagaagcg gcaaacgagt ttcgccgcta cagcaaacgc tttgccgccg
     1441 gtgcacaaaa agaaacggcg gctattttcg atctttactc gcacctgctt tcggacaccc
     1501 ggctgcgtcg cgaattgttt gccgaggttg ataaaggctc ggtggcagag tgggcggtaa
     1561 aaacggtcat tgaaaaattt gccgaacagt ttgccgcgct aagcgataac tatctcaaag
     1621 agcgggctgg cgatttacgt gcgctgggtc agcgattgct gtttcatctt gatgacgcta
     1681 atcaagggcc gaacgcctgg ccggaacgtt tcattctggt ggcagatgaa ctgtcagcga
     1741 caactcttgc tgagctgccc caggatcgct tagtcggtgt tgtcgtgcga gatggcgcag
     1801 ccaactccca tgctgcgatc atggtacgtg cgctggggat ccctaccgtg atgggcgcgg
     1861 atattcagcc ttcggtgctg catcgtagga cgctgatcgt tgatggctat cgcggtgaat
     1921 tgctggtcga tccggagccg gtactgctgc aagaatatca gcggctaatt agtgaagaga
     1981 ttgagcttag ccgtctggcg gaagatgacg tcaatttacc cgcgcagtta aaaagcggtg
     2041 agcgtataaa agtcatgctc aatgctggtt taagcccgga acatgaagaa aaactgggca
     2101 gccgtattga tggcatcggt ctttatcgca ctgaaatccc attcatgctg caaagtggtt
     2161 tcccgtcgga agaagaacag gtggcgcagt atcaggggat gctgcaaatg ttcaatgata
     2221 aacccgtcac cttgcgtacg ctggatgtcg gagcagataa gcagctgcct tacatgccga
     2281 tcagcgaaga gaatccatgc ctgggttggc gtgggattcg cattacgctc gatcagccgg
     2341 agatcttctt gatccaggtg cgggcgatgc tgcgtgctaa tgccgctacg ggcaacctga
     2401 atattctgtt gccgatggtc acaagcctcg atgaagttga tgaagcacgc cgcctgattg
     2461 aacgtgccgg acgtgaagtc gaggagatga tcggttacga aattcccaaa ccacgtatcg
     2521 gcatcatgct ggaagtgccg tcaatggtat ttatgctgcc gcatctggca aagcgggtcg
     2581 atttcatctc tgttggcacc aacgatctga ctcaatacat tctggccgtt gatcgcaaca
     2641 atacccgggt ggcgaacatt tatgacagtc ttcatcctgc aatgttacga gctctggcga
     2701 tgatcgcccg ggaagcggaa atacatggaa tcgatctccg tttgtgcggt gaaatggcgg
     2761 gcgatcccat gtgcgtggca atcctcattg ggcttgggta tcgccatctg tctatgaacg
     2821 gacgttctgt agcgcgggta aaatacctgc tgcggcgcat tgattatgcc gaagcagaaa
     2881 atcttgcgca gcgtagtctg gaagcgcaac tggcgaccga agttcgccat caggttgcag
     2941 cctttatgga gcgtcgcggc atgggcgggc tgattcgcgg agggttatag cgcggatcat
     3001 atacatatct tttaacggta tccggcaacc agccaggtcc ccttgtgcta ttattcgcac
     3061 ctttggagcg cctgaaacct gcggcgcgca tttcaatcgc tgttctcttt cagcgaaata
     3121 acaagaactt gtggtgacag atgaccagta gctatctgca ttttccggag tttgatccgg
     3181 tcattttctc aataggaccc gtggcgcttc actggtacgg cctgatgtat ctggtgggtt
     3241 tcatttttgc aatgtggctg gcaacacgac gggcgaatcg tccgggcagc ggctggacca
     3301 aaaatgaagt tgaaaactta ctctatgcgg gcttcctcgg cgtcttcctc gggggacgta
     3361 ttggttatgt tctgttctac aatttcccgc agtttatggc cgatccgctg tatctgttcc
     3421 gtgtctggga cggcggcatg tctttccacg gcggcctgat tggcgttatc gtggtgatga
     3481 ttatcttcgc ccgccgtact aaacgttcct tcttccaggt ctctgatttt atcgcaccac
     3541 tcattccgtt tggtcttggt gccgggcgtc tgggcaactt tattaacggt gaattgtggg
     3601 gccgcgttga cccgaacttc ccgtttgcca tgctgttccc tggctcccgt acagaagata
     3661 ttttgctgct gcaaaccaac ccgcagtggc aatccatttt cgatacttac ggtgtgctgc
     3721 cgcgccaccc atcacagctt tacgagctgt tgctggaagg tgtagtgctg tttattatcc
     3781 tcaacctgta tattcgtaaa ccgcgcccaa tgggagctgt ctcaggtttg ttcctgattg
     3841 gttacggcgc gtttcgcatc attgttgagt ttttccgcca gcccgacgcg cagtttactg
     3901 gtgcctgggt gcagtacatc agcatgggac aaattctttc catcccgatg attgtcgcgg
     3961 gtgtgatcat gatggtctgg gcatatcgtc gcagcccaca gcaacacgtt tcctgaggaa
     4021 ccatgaaaca gtatttagaa ctgatgcaaa aagtgctcga cgaaggcaca cagaaaaacg
     4081 accgtaccgg aaccggaacg ctttccattt ttggtcatca gatgcgtttt aacctgcagg
     4141 atggattccc gctggtgaca accaaacgtt gccacctgcg ttccatcatc cacgaactgc
     4201 tgtggttcct gcagggcaat actaacattg cttatctaca cgaaaacaat gtcaccatct
     4261 gggacgaatg ggccgatgaa aatggcgacc tcgggccagt gtatggtaaa cagtggcgcg
     4321 cctggccaac gccagatggt cgtcatattg accagattac tacggtactg aaccagctga
     4381 aaaacgaccc ggattcgcgc cgcattattg tttcagcgtg gaacgtaggc gaactggata
     4441 aaatggcgct ggcaccgtgc catgcattct tccagttcta tgtggcagac ggcaaactct
     4501 cttgccagct ttatcagcgc tcctgtgacg tcttcctcgg cctgccgttc aacattgcca
     4561 gctacgcgtt attggtgcat atgatggcgc agcagtgcga tctggaagtg ggtgattttg
     4621 tctggaccgg tggcgacacg catctgtaca gcaaccatat ggatcaaact catctgcaat
     4681 taagccgcga accgcgtccg ctgccgaagt tgattatcaa acgtaaaccc gaatccatct
     4741 tcgactaccg tttcgaagac tttgagattg aaggctacga tccgcatccg ggcattaaag
     4801 cgccggtggc tatctaatta cgaaacatcc tgccagagcc gacgccagtg tgcgtcggtt
     4861 tttttaccct ccgttaaatt cttcgagacg ccttcccgaa attttgcaac gtcctgcaac
     4921 ggcgtaaata gtccggaaga tgcgccgaag aaatagaaac tggccctcgt ctttttttct
     4981 cctcgctcca tactgccggc atgaaaacac aacgtggtta tacgctgatt gaaacgctgg
     5041 tcgcgatgct gattttggtc atgctaagcg caagtggact ctatggctgg caatactggc
     5101 agcagtcgca acggctatgg caaaccgcca gccaggcgcg ggactatttg ctctatttac
     5161 gtgaagatgc caactggcat aaccgcgacc acagtatcag cgttatcagg gaggggacgt
     5221 tatggtgcct tgtgagttcc gctgctgggg ccaatacctg tcatggcagt tcaccattgg
     5281 tctttgtgcc acgctggccc gaagtcgaaa tgagcgacct gacaccttcg cttgctttct
     5341 ttggcctgcg caataccgca tgggccgggc atattcgctt caaaaactca acgggcgagt
     5401 ggtggctggt ggtttcgccg tggggaagac tccggctttg tcagcaagga gaaacagaag
     5461 gatgcctgta aaagagcaag gtttttctct gctggaagtg ttgattgcta tggcgatcag
     5521 tagcgtattg ttgctggggg cggcacgctt tctgcctgcg ttacagcgtg aaagtttaac
     5581 gagcacccgt aagctggcgc tggaagatga aatctggctg cgggtattta ccgtcgcgaa
     5641 gcatctccag agggcgggtt attgtcatgg catctgtacc ggcgaagggc tggaaattgt
     5701 cggacagggt gactgtgtca ttgtgcagtg ggatgcgaac agtaacggta tctgggatcg
     5761 cgaaccggta aaagagtccg accagattgg atttcgtctg aaggagcatg tgctggaaac
     5821 gctacgcggt gcgacatcct gtgaaggtaa gggctgggat aaagtcacta atccggatgc
     5881 catcattatc gacacttttc aggtcgtacg tcaggatgtc agcggcttct cgccggtgtt
     5941 gacggttaat atgcgtgctg ccagtaagtc tgaaccgcaa accgtggtga atgccagcta
     6001 tagcgtgaca ggattcaacc tgtgaaccgc gaaaagggtg tttcgtcact ggctctggtc
     6061 ctgatgctgc tggttttggg tagtttgcta ttacaaggaa tgagccaaca ggatcgcagt
     6121 tttgcctctc gcgtgagcat ggaaagtcag tcattgcgcc gccaggccat cgttcagtcg
     6181 gcgctggcgt ggggaaaaat gcactcctgg cagacgcagc cagcagttca gtgctcgcag
     6241 tacgctgaaa ccgatgccca ggtttgtttg cgtttactgg cagataatga agccttattg
     6301 attgccggtt atgaaggcgt ttcgttgtgg cgaacaggcg aagtcattga tggaaacatt
     6361 gttttttcgc cacgcggctg gagcgatttt tgtccgctga aagagagggc gttatgtcag
     6421 cttccctgaa gaatcaacaa ggctttagcc tgccggaggt aatgttggcg atggtgttga
     6481 tggtgatgat tgtcactgcg ttatcgggtt tccagcgaac attaatgaac agtcttgcca
     6541 gcagaaacca gtaccaacag ctctggcgac atggctggca gcaaacgcaa ctgcgcgcga
     6601 tttcgccacc tgccaactgg caggtcaacc gaatgcagac atcgcaggcg ggatgtgtca
     6661 gcatcagcgt tacgctagtt tcacccgggg gcagagaagg cgagatgacc cgcctgcatt
     6721 gcccgaatcg tcagtagtca ggagccgcta tgttaagggt ctaccattcc aatcgtctgg
     6781 acgtgctgga agcgttgatg gagtttattg tcgaacgcga acggctggac gatcctttcg
     6841 aaccagagat gattctggtg caaagtaccg gtatggcaca gtggctgcaa atgaccctgt
     6901 cgcaaaagtt tggtattgcg gcaaacattg attttccgct gccagcgagc tttatctggg
     6961 atatgttcgt ccgggtgtta ccggaaatcc ccaaagagag cgcctttaac aaacagagca
     7021 tgagctggaa actgatgact ctgctgccgc aattgctgga gcgcgaagac tttaccctgt
     7081 tgcggcatta tctgactgac gatagcgaca agcgaaaact gttccagctt tcctcaaaag
     7141 cggcggacct gcttgaccag tatctggtct atcgtccgga ctggctggca cagtgggaaa
     7201 caggacattt ggttgaaggg ctgggagaag cacaggcctg gcaagccccg ttgtggaagg
     7261 cgctggtgga atatacccat caactcgggc aaccgcgctg gcaccgcgcc aatctctatc
     7321 agcgctttat cgaaacgctg gagtccgcga cgacctgccc gccggggtta ccttcgcgcg
     7381 tctttatatg cggtatttcc gcgttaccgc ctgtttatct ccaggcgcta caggcgctgg
     7441 gtaaacatat tgaaatccat ctcctgttta ccaacccctg ccgttattac tggggcgata
     7501 ttaaagatcc tgcttatctg gcgaaactgc tgacccgtca gcgtcgacac agttttgaag
     7561 atcgcgaatt gccgctattt cgtgatagcg aaaatgccgg gcagctcttt aacagcgatg
     7621 gtgaacagga tgtcggcaac ccgctgctgg cttcatgggg taagcttggg cgcgactaca
     7681 tttatctcct ttctgacctg gagagcagcc aggagctgga cgcctttgtc gatgtcacgc
     7741 cagataacct gctgcataac attcagtctg acattctgga actggaaaac cgcgccgttg
     7801 ctggtgtgaa catcgaagag ttttcccgta gcgataacaa acgcccgctt gatccactgg
     7861 atagcagtat caccttccac gtttgccata gcccgcagcg tgaagttgaa gttttacacg
     7921 atcgcctgct ggcgatgctg gaggaagacc cgacacttac tccgcgcgac atcatcgtga
     7981 tggtggctga tatcgacagc tacagtccgt ttattcaggc tgtgtttggt agcgcacctg
     8041 cggatcgtta cctaccttac gccatttccg accgtcgtgc gcggcagtca catccggtac
     8101 tggaagcgtt tatcagcctg ttatcactgc ctgacagtcg ttttgtgtca gaggatgtgc
     8161 tggcgttgct ggatgtgccg gtgctggcgg cgcggtttga catcaccgaa gaagggctgc
     8221 gttatttacg ccagtgggtc aacgaatccg gcattcgttg gggcatagat gacgacaacg
     8281 ttcgcgagct ggaactcccc gccaccggac aacacacctg gcgatttggc ctgacgcgta
     8341 tgttgttggg ctacgcgatg gagagcgcgc agggcgagtg gcaatcggtt ctaccttatg
     8401 atgaatcgag cggcttaatt gcagaactgg tggggcatct ggcttcactg ctaatgcagc
     8461 taaacatctg gcgtcgcggg ctggcacagg agcgtccgct agaagagtgg ttgccggttt
     8521 gtcgcgatat gctcaacgcc ttcttcctgc cggatgcgga aaccgaagcg gcgatgacgc
     8581 tgatcgaaca acaatggcag gcgattatcg ccgaaggttt aggtgcgcag tatggcgacg
     8641 cggtgccgct gtcactattg cgtgatgaac tggcgcagcg tctggatcaa gaacgtatca
     8701 gccagcgttt tctcgccgga ccggttaaca tttgtactct gatgccaatg cgttcaattc
     8761 cgttcaaagt ggtttgcctg ctgggaatga acgacggcgt ttatccacgt cagcttgcgc
     8821 cattgggctt tgacctgatg agccagaaac cgaagcgtgg cgaccgtagc cgtcgcgatg
     8881 acgaccgcta cctgttcctg gaagcgttaa tttccgcgca gcaaaaactc tatatcagct
     8941 atatcgggcg ttccattcag gataacagtg aacgtttccc gtcggtactg gtgcaggaac
     9001 tgatcgacta catcgggcaa agtcattatc taccgggcga tgaagcgctc aactgtgatg
     9061 aaagcgaggc aagggtaaaa gcgcatctta cttgcctcca tacccggatg ccgtttgatc
     9121 cacaaaacta ccagccaggc gaacgacaaa gctatgctcg tgaatggcta cctgcggcca
     9181 gccaggctgg taaagcacat tctgaatttg ttcagccgct gccgtttacc ttaccggaaa
     9241 ccgtgccgct ggaaacgcta caacgattct gggcacatcc ggtgcgggcg ttcttccaga
     9301 tgcgtttgca ggtgaacttc cgtactgaag acagcgaaat cccagacacc gagccattta
     9361 ttctggaagg acttagccgt tatcaaatca atcagcagtt attgaatgca ctggttgagc
     9421 aggatgatgc cgaacgcttg ttccgccgct tccgggcggc aggggattta ccgtatggcg
     9481 cttttggtga aattttctgg gaaacacagt gccaggagat gcagcagctt gccgacagag
     9541 tcattgcctg tcgccagccg gggcagagta tggaaattga tctcgcctgc aacggtgtgc
     9601 agataactgg ctggttgccg caggtgcagc cggatggcct gttgcgctgg cgtccctctt
     9661 tattaagtgt ggcgcaggga atgcaacttt ggctggaaca ccttgtctac tgtgccagcg
     9721 gtggtaatgg cgaaagtcgc ctttttctac gcaaagacgg tgagtggcgt tttccgccgc
     9781 ttgcagccga acaggctttg cattacctct cacaactgat tgaggggtat cgtgaaggaa
     9841 tgtccgcgcc attgctggtg ttacctgaaa gtggcggcgc gtggctaaaa acctgttatg
     9901 acgcgcaaaa cgatgccatg ctggatgacg attccacgtt gcaaaaagcc cgtacgaaat
     9961 tccttcaggc ttacgaaggc aacatgatgg tgcgtggcga aggtgatgat atctggtatc
    10021 aaaggctctg gcggcaatta acaccagaga caatggaggc catcgttgaa cagtcgcaac
    10081 gtttcctgtt accgctgttt cgctttaatc agtcatgagt gctgtataaa aattgcgcaa
    10141 tctatccgct tactttatga tgcgcaccag tcacggactg atggttatat aaacataggc
    10201 tgactcgtgc agcacaagat taaattctgg cagatgattt gcgttaacgt gttgaatctg
    10261 gacagaaaat taagttgatt atgaggtccg tgaatgcccc gcagcacctg gttcaaagca
    10321 ttattgttgt tagttgccct ttgggcaccc ttaagtcagg cagaaacggg atggcagccg
    10381 attcaggaaa ccatccgtaa aagtgataaa gataaccgcc agtatcaggc tatacgtctg
    10441 gataacggta tggtggtctt gctggtttct gatccgcagg cagttaaatc gctctcggcg
    10501 ctggtggtgc ccgttgggtc gctggaagat cccgaggcgt accaggggct ggcacattac
    10561 cttgaacata tgagtctgat ggggtcgaaa aagtacccgc aggctgacag tctggccgaa
    10621 tatctcaaaa tgcacggcgg tagtcacaat gccagcactg cgccgtatcg cacggctttc
    10681 tatctggaag ttgagaacga cgccttgcct ggtgcggtag accgcctggc cgatgctatt
    10741 gctgaacctt tgctcgacaa gaaatatgcc gaacgtgagc gtaatgcggt gaacgctgaa
    10801 ttaaccatgg cgcgtacgcg tgacgggatg cgcatggcac aggtcagcgc agaaaccatt
    10861 aacccggcac accccggttc aaagttttct ggtggtaacc tcgaaacttt aagcgacaaa
    10921 cctggtaatc cggtgcagca ggcgctgaaa gatttccacg agaagtacta ttccgccaat
    10981 ttgatgaagg cggttattta cagtaataaa ccgctgccgg agttggcaaa aatggcggcg
    11041 gacacctttg gtcgcgtgcc gaacaaagag agcaaaaaac cggaaatcac cgtgccggta
    11101 gtcaccgacg cgcaaaaggg cattatcatt cattacgtcc ctgcgctgcc gcgtaaagtg
    11161 ttgcgcgttg agtttcgcat cgataacaac tcagcgaagt tccgtagtaa aaccgatgaa
    11221 ttgattacct atctgattgg caatcgcagc ccaggtacac tttctgactg gctgcaaaag
    11281 cagggattag ttgagggcat tagcgccaac tccgatccta tcgtcaacgg caacagcggc
    11341 gtattagcga tctctgcgtc tttaaccgat aaaggcctgg ctaatcgcga tcaggttgtg
    11401 gcggcaattt ttagctatct caatctgtta cgtgaaaaag gcattgataa acaatacttc
    11461 gatgaactgg cgaatgtgct ggatatcgac ttccgttatc cgtcgatcac ccgtgatatg
    11521 gattacgtcg aatggctggc agataccatg attcgcgttc ctgttgagca tacgctggat
    11581 gcagtcaata ttgccgatcg gtacgatgct aaagcagtaa aggaacgtct ggcgatgatg
    11641 acgccgcaga atgcgcgtat ctggtatatc agcccgaaag agccgcacaa caaaacggct
    11701 tactttgtcg atgcgccgta tcaggtcgat aaaatcagcg cacaaacttt cgccgactgg
    11761 cagaaaaaag ccgccgacat tgcgctctct ttgccagagc ttaaccctta tattcctgat
    11821 gatttctcgc tgattaagtc agagaagaaa tacgaccatc cagagctgat tgttgatgag
    11881 tcgaatctgc gcgtggtgta tgcgccaagc cgttattttg ccagcgagcc caaagctgat
    11941 gtcagcctga ttttgcgtaa tccgaaagcc atggacagcg cccgcaatca ggtgatgttt
    12001 gcgctcaatg attatctcgc agggctggcg cttgatcagt taagcaacca ggcgtcggtt
    12061 ggtggcataa gtttttccac caacgctaac aacggcctta tggttaatgc taatggttac
    12121 acccagcgtc tgccgcagct gttccaggca ttgctcgagg ggtactttag ctataccgct
    12181 acggaagatc agcttgagca ggcgaagtcc tggtataacc agatgatgga ttccgcagaa
    12241 aagggtaaag cgtttgagca ggcgattatg cccgcgcaga tgctctcgca agtgccgtac
    12301 ttctcgcgag atgaacggcg taaaattttg ccctccatta cgttgaaaga ggtgctggcc
    12361 tatcgcgacg ccttaaaatc aggggctcga ccagagttta tggttatcgg caacatgacc
    12421 gaggcccagg caacaacgct ggcacgcgat gtgcaaaaac agttgggcgc tgatggttca
    12481 gagtggtgtc gaaacaaaga tgtagtggtc gataaaaaac aatccgtcat ctttgaaaaa
    12541 gccggtaaca gcaccgactc cgcactggca gcggtatttg taccgactgg ctacgatgaa
    12601 tacaccagct cagcctatag ctctctgttg gggcagatcg tacagccgtg gttctacaat
    12661 cagttgcgta ccgaagaaca attgggctat gccgtgtttg cgtttccaat gagcgtgggg
    12721 cgtcagtggg gcatgggctt ccttttgcaa agcaatgata aacagccttc attcttgtgg
    12781 gagcgttaca aggcgttttt cccaaccgca gaggcaaaat tgcgagcgat gaagccagat
    12841 gagtttgcgc aaatccagca ggcggtaatt acccagatgc tgcaggcacc gcaaacgctc
    12901 ggcgaagaag catcgaagtt aagtaaagat ttcgatcgcg gcaatatgcg cttcgattcg
    12961 cgtgataaaa tcgtggccca gataaaactg ctgacgccgc aaaaacttgc tgatttcttc
    13021 catcaggcgg tggtcgagcc gcaaggcatg gctattctgt cgcagatttc cggcagccag
    13081 aacgggaaag ccgaatatgt acaccctgaa ggctggaaag tgtgggagaa cgtcagcgcg
    13141 ttgcagcaaa caatgcccct gatgagtgaa aagaatgagt gatgtcgccg agacactaga
    13201 tcctttgcgc ttgcccttac agggtgagcg cctgattgaa gcctctgccg gcacaggcaa
    13261 aacctttacg attgcggcgc tctatttgcg cctgttactt ggactaggcg gttccgccgc
    13321 ctttccccgc ccgctgaccg ttgaagaact gctggtggtc acctttaccg aggctgccac
    13381 ggcagaattg cgtggtcgta tccgtagcaa tatccacgag ttgcgcatcg cctgtctgcg
    13441 tgaaaccacc gacaatccac tgtacgaacg cctgctggaa gagatcgacg ataaagcgca
    13501 agccgcgcag tggttgttgt tagccgaacg gcagatggat gaagcggcag tctttactat
    13561 tcacggtttt tgccagcgca tgctcaacct gaatgccttt gaatccggta tgctgtttga
    13621 gcagcagctg attgaagatg agtctctgct acgctaccag gcctgcgccg atttctggcg
    13681 tcgccactgc tacccgctgc cgcgtgaaat agcccaggtc gtctttgaaa cctggaaagg
    13741 gccgcaggcg ttgctgcgcg atattaatcg ttatctgcaa ggcgaagcgc cggttatcaa
    13801 agcaccgccg cccgatgatg aaacgctggc ttcccgccac gcgcaaattg tggcgcgtat
    13861 tgatacggta aaacagcagt ggcgcgacgc agtgggtgaa ctggatgcgc tgatcgaatc
    13921 ttctggtatt gatcgacgca agtttaaccg tagcaatcag gctaaatgga tcgacaagat
    13981 cagcgcctgg gcagaagaag agaccaacag ttatcagttg ccagagtcgc tggaaaaatt
    14041 ctcccagcgt ttcttagaag atcgcacgaa agccgggggg gaaaccccgc gacatccact
    14101 gtttgaggcg atcgatcaac tgcttgcaga accattgtcg atccgcgatc tggtgatcac
    14161 ccgcgcattg gctgagatcc gcgaaacagt agcgcgtgaa aaacgccgcc gtggcgaatt
    14221 gggttttgat gacatgttaa gtcggctcga ttccgcgctg cgtagcgaaa gcggtgaggt
    14281 gttggcagcg gcgatccgta cgcgattccc ggtggcaatg atcgatgaat ttcaggatac
    14341 cgacccccag cagtaccgaa tttttcgccg tatctggcac catcagccgg aaaccgcatt
    14401 gttgctgatt ggcgacccga agcaggccat atatgcattc cggggtgcgg atatcttcac
    14461 ttatatgaag gcgcgtagcg aagttcacgc ccactacact ttagacacca actggcgttc
    14521 cgcaccagga atggtgaaca gcgtgaataa gcttttcagc cagactgatg acgcgttcat
    14581 gtttcgcgaa ataccgttta ttccagtgaa atcagccggg aaaaatcagg cgttacgttt
    14641 tgtatttaaa ggtgaaacac agcctgcgat gaaaatgtgg ctgatggaag gcgaaagctg
    14701 cggcgttggc gattatcaaa gtaccatggc gcaggtatgt gctgcgcaaa tccgcgactg
    14761 gctacaagcc ggacagcggg gcgaagcgtt gctgatgaac ggcgacgacg cgcgtccggt
    14821 gcgtgcttcg gacatcagtg tgctggtgcg cagccgcaag gaggccgccc aggtgcgcga
    14881 tgccttaacg ttgctggaaa tcccttccgt ttacctttcg aaccgcgaca gtgtttttga
    14941 aactctggaa gcgcaggaaa tgctttggtt gttgcaggcg gtgatgacgc ccgaacgtga
    15001 gaacaccctg cgtagtgcgc tggcaacgtc aatgatgggg ctgaacgcgc tggatatcga
    15061 aacgctgaac aatgacgaac atgcgtggga tgtggtagtc gaagagttcg atggttatcg
    15121 gcaaatctgg cgcaaacgtg gcgttatgcc gatgctgcgg gcgctgatgt cggcgcgtaa
    15181 cattgctgaa aacttgctgg caacggcagg cggtgagcgg cgtcttaccg atatcttgca
    15241 tatcagcgaa ctgctacaag aagccggaac gcagctggaa agtgaacatg cgctggtacg
    15301 ctggttatcg caacatatcc tcgagccaga cagtaatgcc tccagccaac aaatgcgtct
    15361 cgaaagtgat aaacatctgg tgcagattgt cacgatccac aaatcgaaag ggctggaata
    15421 tccattggtc tggctgccgt ttatcaccaa tttccgcgtc caggagcagg cgttttatca
    15481 cgatcgccac tcgtttgagg cagttctgga tcttaatgct gcgccagaaa gcgtcgacct
    15541 cgcggaggcc gaacgtctgg cggaagatct gcgtttgctt tacgtggcgc tgacacgttc
    15601 ggtttggcat tgcagtctcg gcgttgcacc gctggtgcgc cgtcgtggcg ataaaaaagg
    15661 tgacaccgac gtccaccaaa gtgcgctcgg gcgtttgctg caaaaagggg aaccgcaaga
    15721 tgcggcaggg cttcgcacct gtattgaagc gttatgcgat gatgatattg cctggcaaac
    15781 ggcacaaact ggtgataacc aaccctggca ggttaatgat gtttctacag cagagctgaa
    15841 tgcgaagacg ttacaacgat tgcccggcga taactggcgc gtcaccagct actctggttt
    15901 gcaacagcgt ggtcacggta tcgcccagga tttgatgcct cggctggatg tcgatgctgc
    15961 aggcgttgcc agcgtcgttg aagaaccgac gttaacacca catcagtttc cgcgcggtgc
    16021 gtcaccgggg acgttcttgc acagtttgtt tgaagacctg gattttaccc agccggttga
    16081 cccgaactgg gtgcgggaaa aactggaact cggcggcttt gaatcgcagt gggaaccggt
    16141 attgaccgag tggatcacgg ctgtcctcca ggcacctctc aatgaaaccg gcgtaagcct
    16201 gagtcaactt tccgcccgca ataaacaggt ggagatggag ttttatctgc cgattagtga
    16261 accgcttatc gccagtcagc ttgatacgtt aatccgccag tttgacccgc tatccgcagg
    16321 ctgcccgccg ctggagttca tgcaggtacg tggcatgtta aaaggcttta tcgacctggt
    16381 gttccgccac gaagggcgtt attacctgct cgactataaa tccaactggt tgggtgaaga
    16441 cagttcggct tacacccaac aggctatggc agcggcaatg caggcacacc gctatgatct
    16501 gcaatatcag ctttataccc tggcgctgca tcgttatctg cgccatcgca ttgctgatta
    16561 cgactatgag caccactttg gcggcgttat ttatctgttc ctgcgtggcg ttgataaaga
    16621 acatccgcaa caggggattt acacaacccg acccaacgcc gggttgattg ccctgatgga
    16681 tgagatgttt gccggtatga ccctggagga ggcgtaatga aattgcaaaa gcaattactg
    16741 gaagctgtgg agcacaaaca gctacgcccg ctggatgtgc aatttgccct gaccgtggcg
    16801 ggagatgaac atcctgccgt caccctcgcg gcggcactgt taagtcatga tgccggagag
    16861 ggacacgttt gtttgccgct ttcacgactg gaaaataacg aggcgtcgca tccgctgttg
    16921 gcgacctgtg tcagtgaaat cggtgagcta caaaattggg aagaatgctt gctggcttct
    16981 caagcggtca gcaggggaga tgaacccacg ccgatgatcc tctgtggcga tcgtctttat
    17041 ttgaatcgca tgtggtgtaa cgagcgcaca gtggcacgct ttttcaacga agtgaatcat
    17101 gccattgagg ttgatgaagc tctactggcg caaaccctgg acaaactttt tccagtaagc
    17161 gatgaaatta actggcaaaa agttgcggcg gcagtggcgc tgacgcggcg gatctcggtg
    17221 atttccggcg gccctggcac cggtaaaacg accaccgtag cgaagttgct ggcagcgtta
    17281 attcaaatgg ccgacggcga acgctgccgt atccgtctgg ctgcaccaac gggtaaagct
    17341 gccgcgcgct taaccgaatc tctcggcaag gctttgcgac agttaccgct gaccgatgaa
    17401 caaaagaaac gcattccgga agatgccagc actttgcacc gattgctggg cgcgcagccg
    17461 ggtagccagc gtttacgtca tcatgccggt aacccgctgc atcttgatgt gctggtggta
    17521 gatgaagcgt caatgatcga tctgcctatg atgtcgagac tgatcgacgc cttgcccgat
    17581 catgcgcgag tgatctttct cggcgatcgt gatcaactgg cctcggttga ggctggggct
    17641 gtgctgggcg atatctgcgc ttatgccaac gcgggcttta ccgccgagcg tgccaggcag
    17701 ctaagccgcc tgacggggac tcacgttccg gcaggaaccg gcacagaagc ggcatctttg
    17761 cgcgacagtc tctgcctgct gcaaaaaagc tatcgtttcg gcagcgattc tggcattggt
    17821 cagttagctg cggcgatcaa ccgtggtgat aaaacggcag tgaaaaccgt ttttcagcag
    17881 gattttactg atatcgaaaa acggctttta cagagcggcg aagattatat tgcgatgctt
    17941 gaggaagctc ttgcgggtta cggacgttat ctggatctgc tgcaagcgcg tgccgagccg
    18001 gatttaatca ttcaggcgtt caatgagtac cagcttttgt gcgccctgcg ggaagggccg
    18061 tttggcgtgg ctggactgaa tgagcgaatt gagcagttta tgcaacagaa gcgcaaaatt
    18121 catcgtcatc cgcactctcg ttggtacgaa ggtcgaccgg tgatgattgc ccgtaatgac
    18181 agcgcgcttg ggttgtttaa tggcgatatc ggtattgcgc tggatcgcgg gcaggggacg
    18241 cgcgtctggt ttgcgatgcc ggacggcaat attaagtctg tgcaaccgag tcgcttgcca
    18301 gagcacgaaa ctacgtgggc gatgacggta cataaatcgc agggatcgga gttcgaccat
    18361 gcggcgttga ttttgccgag ccaacgcacg ccggtagtaa cgcgagagct ggtttatacc
    18421 gcggtgaccc gcgcgcgtcg ccgtctgtcg ctgtatgccg atgagcgcat attaagtgcg
    18481 gcaatcgcca ctcgtactga gcggcgcagt ggtctggcgg cgttgtttag ttcacgggaa
    18541 taaacgtaat tgccggatgc gacgtacgag tgttacgcat gtcgcatccg acgattttca
    18601 tcgcttaccc taaatccgcc atcaacactt tggatttacg ctggtagttg tacaactgct
    18661 ttttgctctc gggcagtaaa tcaatatcca ctggggtaaa tccacgttcc tggaaccagt
    18721 gaatactgcg cgtggtcagc acaaacaatt tgcttaagcc gctctgcttc gcctgagcgg
    18781 caatgcgttc cagcagaact tcaccccttg atgaactgcg gtaatccggg tgaactgcca
    18841 cacaggccat ttccccaatc ttctcttccg ggaacggata gagcgcggcg caggcaatag
    18901 tcgtgttatc gcgctgaata atggtgaatt tgtcgatttc catctccagc tgctcgcgag
    18961 aacggcgtac cagaatacct tgctgctcca gtgggcgaat caactccaga ataccgccaa
    19021 tatcgttgat tgttgcgcga cgaatctgct cggcgctttc catcacaatc tgcgtaccga
    19081 taccgtcgcg tgagaacaac tcttgcaaca gcgcgccatc ttcctgataa ctgattaaat
    19141 gacagcgacg cacgccgctg cggcaggctt tcactgcgcc acgcaaaaag cgcaccgtac
    19201 cggagttgta atcgcctttc tcttcctggg cttctacccg cgcttgcgct tcgttaggga
    19261 aaagttcgga gacaatatca ccgtcgtcat tagtgacgcc ctgggaagag caaaaaccaa
    19321 tcatcttttc agctttcagt ttgatggcca gttgagtggc aatctcttcc gaggtcaggt
    19381 taaagctctc gccagtgact gaaacagcga ccggccccat tagcactatt gcaccgctgt
    19441 ccagttgacg atggatcgcg tcttcatcaa tccgccggat acgcccgcta tggcagtaat
    19501 ccacgccgtc atcgacgccc agcggctggg caataataaa attgccactg acgacgttga
    19561 tatgcgcgcc ctgcagcggc gtgttattga gactcatcga caggcgagca gtaatatcca
    19621 gttgcaatgt tcccgcagcc tgcttcacca gttccagtgt tttggcgtcg gtcacacgta
    19681 tattcttgtg atacagcggt tcgtggtgat gcgcagccag atttgcgtcg atctgcggac
    19741 gtgcgccata gaccaccacc agacggatgc cgaggctgtg caacaacccg atatcattaa
    19801 cgatactgga gaaattctca tgctcaatgg cttcaccgcc gagcatgatg acaaacgttt
    19861 ttccccggtg ggtattgata tagggaaccg aatggcggaa tccctcgacc aactcggttt
    19921 tacgttcctt taccacggca cacctctttg catgattatt cgaaattagt gtatttttat
    19981 tctgtttttt cgcagggtgc aagtgtaaat tttatgcgag agcgactttt ttatcagtaa
    20041 tgccgtgaat ataaaaagaa tgtttaccgt ttattagatg acagattatg cgtctttcgc
    20101 taaagtttcc ggtcaaatta gtcgtttact tgttacacag cttagatttc ttttgctcag
    20161 gagaggcatg tcaggatcca acactgcaat cagccgtcgt cgtttactgc aaggcgcggg
    20221 tgccatgtgg ctattgagcg taagtcaggt cagtctggct gcggtcagcc aggtcgtggc
    20281 ggtgcgcgtc tggcctgcgt ccagctacac ccgcgtgacg gtggaatcaa atcgtcagct
    20341 gaaatataag cagttcgcgt tgagtaaccc tgaacgcgtg gtggtggata tcaaagatgt
    20401 aaacctgaac tcggtactca aggggatggc ggcacaaatt cgtgcagacg acccgttcat
    20461 caagtcggcg cgcgtcgggc aatttgaccc gcaaaccgta cggatggttt ttgaattaaa
    20521 gcaaaacgta aaaccgcagc tgtttgccct tgcgccggtc gccgggttta aagagcgtct
    20581 ggtgatggac ctctatccgg ccaatgcaca ggatatgcag gacccgctgc tggcgctgct
    20641 ggaggattac aacaaaggcg acctcgaaaa gcaggtgccg ccagcgcaaa gtggtccaca
    20701 accgggtaaa gcagggcgcg atcgtccgat tgtcattatg cttgaccctg gccacggtgg
    20761 cgaagactcc ggtgcggtgg ggaaatacaa aacgcgcgaa aaagatgtgg tattgcaaat
    20821 agctcgccgt ttgcgctctc tgatcgagaa agagggcaat atgaaggtgt acatgacgcg
    20881 caatgaagac atcttcattc cattgcaagt gcgcgtagca aaagcccaga aacagcgtgc
    20941 tgacctgttt gtctctatcc atgccgacgc ctttaccagt cgtcagccga gcggttcctc
    21001 tgtgtttgcg ctctcaacca aaggtgcaac cagtactgcg gcaaaatatc tggcacaaac
    21061 ccagaacgcc tcggacttga ttggtggcgt gagcaaaagc ggtgaccgct atgtcgacca
    21121 caccatgttc gatatggtac agtcgctgac cattgccgac agcctgaagt ttggtaaagc
    21181 ggtactgaat aagctcggta aaatcaacaa gctgcataaa aatcaagttg aacaggccgg
    21241 gtttgccgta ctaaaggcac cagatattcc ctccattctg gtcgaaacgg cgtttatcag
    21301 taacgttgag gaagagcgta aactgaaaac ggcgactttc cagcaggaag ttgcggagtc
    21361 tattcttgcg gggattaaag cgtattttgc cgatggggcg acgctggcga gaaggggatg
    21421 ataaaaaggc gctgaatggc gcttttttat gggagaccag aaacaaaaaa acacccgtta
    21481 gggtgttcaa taattggttg cgggggccgg atttgaaccg acgaccttcg ggttatgagc
    21541 ccgacgagct accaggctgc tccaccccgc gtccgtggat gcgcactata ctccgctcgc
    21601 gttttcatgc aacctttttt ttaccccgat cacggatttg gcatgatttt gaacaaaaac
    21661 aaatcactaa agcctatttt ttgtgcaaaa tttgcgtcag ggagaggctg ctcatttcat
    21721 taaacgcggc ggtttgttat cttcgttgcg ccttattttt taacctgaag aagagaacaa
    21781 tgaaaggacg ttgggtaaag taccttctta tgggcacggt tgtggcaatg cttgccgcct
    21841 gctcttccaa accaaccgat cgcggacagc aatataaaga cgggaaattt acccagcctt
    21901 tctctctggt gaaccagcca gatgccgttg gcgcgccgat taacgccggt gattttgccg
    21961 agcaaattaa ccatatccgt aattcgtcac cgcgtctgta tggcaaccag agtaatgttt
    22021 ataacgcggt acaagagtgg ctgcgcgcag gcggtgatac ccgcaatatg cgtcagttcg
    22081 gcattgatgc ctggcagatg gaaggtgccg acaactatgg taacgtgcag tttaccggtt
    22141 attacacacc agtaattcag gcgcgccata cccgccaggg cgagtttcag tatcctattt
    22201 accgtatgcc gccaaaacgt ggtcgtctgc cgtctcgtgc ggagatctac gcgggtgcat
    22261 tgagcgataa gtacatcctc gcttacagca actctctgat ggataacttc attatggatg
    22321 tgcagggtag tgggtatatc gactttggtg atggcagtcc gcttaacttt ttcagctatg
    22381 cagggaaaaa cggtcatgcc tatcgcagca ttggtaaggt gctgatcgac cgtggcgaag
    22441 tgaaaaaaga agatatgtcg atgcaggcga ttcgtcactg gggcgaaaca cacagtgaag
    22501 ccgaggttcg cgagctgctg gaacagaacc cgtctttcgt cttctttaaa ccgcaatctt
    22561 ttgcaccggt gaaaggggca agtgcggtgc cgctggttgg ccgtgcttct gttgcctctg
    22621 atcgttccat tattccgcca ggtacaacct tgctggcaga agtgccgctg ctggataata
    22681 acggcaaatt taatggtcag tacgaattgc gtctgatggt ggcgctggat gtcggtggcg
    22741 caatcaaagg ccaacacttc gatatctatc aagggatcgg gccggaagcc gggcaccgcg
    22801 caggttggta caaccactat ggacgtgtct gggtgctgaa aaccgccccg ggcgcaggta
    22861 acgtctttag cggctgatgt gatattctgt atgccgtttt tacggatatg acagggtgag
    22921 ggataacctc acatcctgat tattgactaa gtgcgcgttg ttcatgccgg atgcggcgtg
    22981 aacgccttat ccggcctaca aatcatgcaa atccaaaata ttgcaggagt tatgtaagcc
    23041 tgataagcgt agcgcatcag gcatttatgc gtttagggtg agggataacc tcgccctttt
    23101 taattctgag gtgctatgtc tgtggtaatt agtgatgcat ggcgtcagcg ttttggtggc
    23161 acagcgcgtc tgtatggtga aaaagcgttg caactgtttg ctgacgcgca tatttgtgtg
    23221 gtcggtattg gcggtgtcgg ttcctgggcg gcggaagcgc tggcgcgcac ggggattggc
    23281 gcaatcacgc ttatcgatat ggatgatgtg tgcgtcacca ataccaatcg gcaaattcat
    23341 gccctgcgcg ataacgttgg gctggcaaaa gcggaagtca tggcggaacg tattcgccag
    23401 attaacccgg agtgccgtgt aacggtggtg gatgatttcg tgacgccgga taacgtagcg
    23461 cagtatatga gcgtgggtta ttcgtacgtg attgatgcca ttgatagtgt acggcccaaa
    23521 gcggcgctga ttgcttattg tcggcgcaat aaaatcccgc tggtcacgac cggtggcgcg
    23581 ggtgggcaga ttgacccgac gcagattcag gttaccgatc tggcgaaaac gattcaggat
    23641 ccgctggcgg cgaagttgcg cgagcgcctg aaaagcgatt ttggcgtagt gaaaaacagt
    23701 aaaggtaagc tcggcgtgga ttgcgtgttt tctactgaag cgctggtgta cccgcagtca
    23761 gacggtacgg tgtgtgcgat gaaagccacg gcagaagggc caaagcggat ggattgtgca
    23821 tctggatttg gcgcggcaac gatggtgacc gccacctttg ggtttgttgc ggtttctcat
    23881 gcgctgaaga agatgatggc gaaagcggcg cgtcagggtt aaacaatcag gtcggatgag
    23941 acgcgaacgt cttatccgac ccggttctgt gcttaaacct gcttcgtcgc agcgataatc
    24001 gcctcgctta acgcatttaa cccctggcta cgtgaggcgc taagctgcgc acgcaatccc
    24061 agctcatcaa acaatgccag cggcgactgt gcctgcaatt cggcggcggt tttcccctca
    24121 acggcagtca acagcaccgc cagcaggccg cgcactatac gtccttcgct gtcgccaaag
    24181 aagtgcattt taccgttttc agccacggta tatcccagcc agacgcggtt ttcgcatccg
    24241 gcaatctctt tagcctgcgc ttttaactcg tctggcaaag ccggaagctg tttccccagc
    24301 atgatcaact ggcgatattt atcttcccat tgcgtcaacg gtgcgaaggt attgcgtaac
    24361 gtttctgcgg ttacggttgt gccgaacgga tgtccggcga attgcgggtt cgtcattaat
    24421 ccaccaataa ttccagcgcg cggtcaacgg cattcaccag cgcatccaca tcactctttg
    24481 tattatatgg cgcaaaagag gcgcgcagtg tgccggttac gcctaattct gccagtagcg
    24541 gctgagcgca atgctgcccg gcgcgcaggg caataccgta ctccgccagc agcgtcacca
    24601 tatcgctgtg atgaacgcca gcaaaatcaa aggccagcag gctggaatcc tggcagcgga
    24661 atgaacgaaa gcctggacgt tttgccagcg catcttccgc cagcgtcgcc aggctgcgac
    24721 tccaactttc tgcctggttg atatcgtaat ctgccagcca ttccagcgcc gcgcttaatc
    24781 ctatgacacc agccacattt ggcgttcctg cttccagttt ccacggcgca gattgagtcg
    24841 tgaagccgtc aaaactcact tcgtgaatca ttttgccgcc acccagccag ggcgacatcg
    24901 cttccagcag ttctgatttg ccatacagca cgccgatacc cgtcgggcca tacagtttgt
    24961 gaccagagaa agcatataaa tcgatatcca gttgctgaac atctgcgggg aaatgcaccg
    25021 ccccctgagc accatcaacc atcaccacca tcccggctga atgagcaaag gtaatcgctc
    25081 gcgccagatc cgggcaaccg ccagtaacgt tcgacatctg acccaacgcc agaatccgac
    25141 tacggggagt aatcagttct ggcaacaaat cgacatccgg cagtcgctgc gcattaagcg
    25201 gcaatttcac cactttggct ccagtttgtt gggcgaccat cagccagggg acgaggttag
    25261 cgtggtgttc tgccacgctg acaataatct catcgcccgg ttgcagacgc ggacgcgcat
    25321 agcattgtgc caccatgttg atggattcag tggtgccgcg cgtccagacg atagttttat
    25381 catccggtgc attcagtaat tgcgccactt tctctcgtgc agcttcataa cgcgtggtca
    25441 ggcgttgggc ttcggcaaac tggctgcgat ggacgtttcc ggcgctcaga ctgtaaaact
    25501 gttgggtggc ttcaaccacg gcttcaggtt taagcgcggt cgcggcgctg tcgagataga
    25561 cgcccgcatc ctgtagtgcg ggaaactggg cgcgaaactg cgctggatta aaaacgttca
    25621 tggtactcct cggcttgatg caccgatcgt gacgcaaaat tcactttgat acaaggagag
    25681 tgctaaccat cagaattatc tggatatcct ggcgaagccg cgtaagtaac atatgctgaa
    25741 tagtgttagg cgttaatttt agcctgttaa tcaatactat taagatttat taatcgcttt
    25801 aaggagaaga tgatgaaaaa gactgccgca attatttctg cctgtatgct gacttttgcc
    25861 ctgagcgcct gttccggttc gaactatgtg atgcacacca atgacggacg taccatcgtc
    25921 tctgacggca aaccacagac tgataacgat accggtatga tttcgtataa agacgctaat
    25981 ggcaataaac agcagatcaa ccgtactgac gtgaaagaga tggtcgaact ggatcagtaa
    26041 ttcgcgatcg caaggtaaaa aaaagcaccg caattaggcg gtgctacatt aatcactatg
    26101 gacagacagg gtaaatgtac aggaagtgaa aaaaggtagc tttgctacca tggtctgaat
    26161 cgcagaccaa ttgcaaacac aacaacacaa catcacaacc gtaagccaaa agttcaccag
    26221 aacacgcatt ccgataaaac ttttcgttcc ggctcaggaa gtgccgccac tataggtatt
    26281 tgctggtaga agctcaacgg acaatttata atggctcaga ttaaaaaaac taataggtta
    26341 catagtgtga tctaattgtt aaattcattt aacatcaaag tttaaaagcc atgtctaaac
    26401 gattaccacc gctaaatgcc ttacgagttt ttgatgccgc agcacgccat ttaagtttca
    26461 ctcgcgcagc agaagagctt tttgtgaccc aggccgcagt aagtcatcaa atcaagtctc
    26521 ttgaggattt tttggggcta aaactgttcc gccgccgtaa tcgttcactc ctgctgaccg
    26581 aggaagggca aagctatttc ctcgatatca aagagatatt ttcgcaatta accgaagcga
    26641 cgcgtaaact ccaggcccgt agcgccaagg gggcgttgac ggtcagttta ctccccagtt
    26701 tcgccattca ttggttggtt ccgcgacttt ccagctttaa ttcagcttat ccgggaattg
    26761 acgttcgaat ccaggcggtt gatcgtcagg aagataagct ggcggatgat gttgatgtgg
    26821 cgatatttta tggtcggggc aactggccgg ggctacgggt ggaaaaactg tacgccgaat
    26881 atttattgcc ggtgtgttcg ccgctactgc tgacaggcga aaaacccttg aagaccccgg
    26941 aagatctggc taaacatacg ttattacatg atgcgtcacg ccgtgactgg cagacatata
    27001 cccgacagtt ggggttaaat catatcaacg ttcagcaagg gccaattttt agtcatagcg
    27061 ccatggtgct gcaagcggct attcacgggc agggagtggc gctggcaaat aacgtgatgg
    27121 cgcaatctga aatcgaggcc ggacgtcttg tttgcccgtt taatgatgtt ctggtcagta
    27181 aaaacgcttt ttatctggtt tgtcatgaca gccaggcaga actgggtaaa atagccgcct
    27241 ttcgccaatg gatcctggcg aaagccgctg ctgaacaaga aaaattccgc tttcgttatg
    27301 aacaataatt tacgtagggt acgaccatga ccagccgttt tatgctgatt ttcgccgcca
    27361 ttagcggctt catttttgtg gctctgggcg cttttggcgc gcatgtgtta agtaaaacca
    27421 tgggggccgt tgagatgggc tggatccaga ccggcctcga ataccaggcg tttcatactc
    27481 tggcgatctt aggtctggcg gtggcaatgc agcgtcgcat cagtatctgg ttttactgga
    27541 gtagcgtttt cctcgcgtta ggcacggtgc tgttcagcgg cagcctttat tgcctggcac
    27601 tgtcccatct gcgtttgtgg gcgtttgtca ctccggttgg cggcgtgagc ttccttgcgg
    27661 gctgggcgtt aatgttagtt ggtgctatcc gtttaaagcg caagggcgta agtcatgaat
    27721 aaggttgtat tgctgtgccg tccgggcttt gaaaaagagt gcgccgcaga aattaccgat
    27781 aaagccggcc agcgggaaat tttcggtttt gcccgcgtga aagagaatgc gggttatgtc
    27841 atttatgaat gttatcaacc tgatgatggc gataagttaa tccgtgagct gccgttcagt
    27901 tcattaattt ttgcccgcca gtggtttgtg gtgggggaac tcctgcagca tttgccgcca
    27961 gaagatcgta ttacccccat tgtcggcatg ttacagggcg tagtagagaa gggcggtgaa
    28021 ctgcgtgttg aagttgccga taccaacgaa agcaaagagt tgctgaaatt ctgccgtaaa
    28081 ttcaccgtgc cgctacgcgc tgccttgcgc gatgccgggg tgctggcgaa ctatgaaacg
    28141 ccgaagcgtc cggtagtgca tgtattcttc attgcaccag gctgctgcta taccggttac
    28201 tcatacagca acaataattc gccgttctat atgggcattc cgcgcctgaa atttccggca
    28261 gatgcgccga gtcgttctac gctcaaactg gaagaggcat ttcatgtgtt tattcctgcg
    28321 gatgagtggg atgaacgcct ggcgaacggg atgtgggcgg tggatttagg cgcttgccct
    28381 ggcggctgga cctaccaact ggtgaagcgc aacatgtggg tttattccgt cgacaacggc
    28441 ccgatggcgc aaagtctgat ggataccggg caggtgacgt ggctgcggga agacggtttc
    28501 aaattccgtc cgacacgcag caatatctcc tggatggtat gcgatatggt tgaaaaaccg
    28561 gctaaagttg cggcattgat ggcgcagtgg ctggttaatg gctggtgccg tgaaaccatt
    28621 ttcaatttca aactgccgat gaaaaaacgc tacgaagaag tatcacacaa tctggcgtat
    28681 attcaggcac agcttgatga acatggcata aatgctcaga ttcaggcgcg gcagttgtat
    28741 cacgatcgcg aagaagtgac ggtgcacgtc cgccgcatct gggctgcggt gggtggtcgt
    28801 cgcgacgagc gataacaggc aaggccgggg cgtcgtcttc ggcctgctac cctttccgtc
    28861 aggctgttac caaagaagtt gcaacctggc gctcatcaga aataatgtgc gttacctcat
    28921 ccagatgccc gatgcgggct tcccctgaac gattaaattt acttttatca atcaataaca
    28981 acgattgcgc ggcacgtttt aatagcatcg atttgtaatc agcgttgatc gcattggagt
    29041 cccacagtgc gccgctgcta tcgatccctt cacaagaaaa aataaacaga tcgatttcca
    29101 gcgatttaag ttgggaaatc agcgagggat tgacgtaaca gccatatttg cgctcaagtg
    29161 tgccgccgga actgatcagt tgaatgcgtt cgcgtttacc gagttcatgg caaatcggat
    29221 ggctattggt gaagacctga atgttgatgt caggcaactg gcgtgccaga taccagcaag
    29281 ttgaactggc gtctaaggct atcaccatcc cttcttcaat ccacgcgagc gcctcgcgcg
    29341 cgatatctgc tttatgcgca taatggcttt tcagcctgat gtgaaaggga tcgccactgt
    29401 cttgattttg acggtggata tatttagcgc gtccatgatt gcgcagaatt ttaccctgcg
    29461 tctgtaattc attgagatcg cgacgaatgg tttctttact gacctttagc tgttcagaga
    29521 gagcttccgt ggtcaggctg gtatggttca gcagcaggtc gactatcgct tgctggcgtg
    29581 ccgctttcat atttttcacc ccatcattcg ataaccgccc gcgtcatgcg ggcgtaccgg
    29641 tatgagatta cggtgttacc ccttttttta aaagaatatt cccgtacttc gctcgttcgc
    29701 ctgtgataac gatcgcaaag gctttttgcg cccgttcata aaacgcaaaa cgattgatgc
    29761 ggatgatgtc aggacacggg gcttgtagtg aaagcgcatt acggtaacgt cgttctactt
    29821 caggatcgag agtgtcacct tctaccgccg ccatcatcac cagcggcggt gcataactgt
    29881 ccagttcaaa taacgggata atcgcctgga gcaagtcgct caccaacagg ccatcagcgc
    29941 ggatcacctg cggtcccatc gaatgggcgg gaaagtgagc atcggaaaaa ataatttcat
    30001 ctccatgtcc catctctgcc agcactttca atagttcggg agaaattaac ggcgaaattg
    30061 ttttcagcat ttcacacttc ctctataaat tcaggttcag tttgcgggta gaaataacgg
    30121 tactgataat gaatctgcgc gcgggcttct tccgggctgt taaattcccc tatgccatac
    30181 caaccgaaca gcgcagctcc tgcgacggtc gtttcggcgt cgtcgagaac ttttaccgga
    30241 atatcaagca tattggcttt aatctgattc cacaatgtgt tgcgacttcc tccgccgact
    30301 aacaataatt cagaggcttt aaagtgcccg attttttcca gcatctgtag attgcgctgt
    30361 aattgcgcgg ttaacccttc cagcgccgcg cgatagaaat gcccccgcgt ggtattaagc
    30421 gtcactcctt gccagccagc gttctgacac gacaataaat cacactgcat ttttacgcca
    30481 tccgcgccag gcgcgatcag acgagcctct tcaatcaaca tttgccaggg tgtttcagcc
    30541 gtccagaaca gttttctcac ccattccagc acgccggatg ccagccattg catacctggg
    30601 ttatacaacc ctgcctggct atccagttcg caggtggaac cggcgtactg acttaacagc
    30661 gaagtatcaa cctgggcgct acggaccatt aaaatttccc atgtaccgga agagagcacg
    30721 ggttcatttt ggtcggcacc agcgccaaaa agggcgaact gggtatcgtg acctgcggaa
    30781 atcaccggta tgccaacggg taagccgagc attgctgcgg cgctgttctg tagcgtacca
    30841 atctgttcac ccgcttccac cagacgaggg aagagtcggc gtggaatacc ggtggcttgt
    30901 aaaatttgcg gactgaaatc gcgttgctgg atatccagca tctggctggt tccggccatc
    30961 gtgatatcag tagtgaattc gccggttaaa cggtggttaa tcagcgacga aataaagagc
    31021 caggcgtgcg cgcgttccag cagttgtgga tgattttctt tcaaccacac caacttatat
    31081 aacgtattga aactaaaggc tccgacgcca gaaatagcct gcaaccgctg tgcggagatc
    31141 aactgttcaa tattgtccat aaccgctgct gttcgcggac atttccagct aatgatcgga
    31201 tagagcagat tgccttgctt atctaccaga gcgccatcca caccaaaggt ggtgacggcg
    31261 ataccgcgga tgtggcattc agtcagttca ctattgattt gccgacagca atcagcaaag
    31321 cgttgcaaaa tggcgtctaa agaccactgg tgccaggtgt tgttttccat cgcgatatcg
    31381 ctggcattag gcgttgaggc gcgggcaaca atttttccct gccgattaac cgcgatggcc
    31441 ctgacattgg tcgcgccaca gtcgagtacc aggataactt cttgtttcat aatcgctcct
    31501 gcaatatagc cggataacat tgcttatccg gctaaccact cttgcttgtt caggcccggt
    31561 agccggagcg accgggcatc acatcaggga gtaatgtatt aacgcttgta caacgggccg
    31621 tagttctggc aagcgcggta atcctggcct tcaatatcca tgccgtgcgc agcccaggca
    31681 gaaggacgat acactttggt ctcttcaacg ttgtgcatac ataccgggat acgcagcatg
    31741 gaggcgagag tgataaagtc agcgccaacg tggccgatgg tcagaacccc atggttagcg
    31801 ccccagttcg ccattaccga gtacacatcc gtaaacggcc ctttaccggt gaggcgcggt
    31861 gcaaaccagg tggttggcca ggttgagttg gtgcgtttgt tgaggatgtc atgcacatcc
    31921 ttcggcaatt ccacgctcca gccttccgcg atttgcagta ccggtcccag gcctttgatg
    31981 atgttgacac gagtcatggt gaacgggacg ccgccttcgg taaggaagcg ggaagagtaa
    32041 ccgccgccac ggaagtattc gtggatcgcc gggcaccatt cggtagcggc gaggcaagcg
    32101 tcagcctctt gctgagagat ttcccagtgc ggcttcatcg tcgggttacc ttcgctgtcg
    32161 cgttgtttac aggaaccgtc cagcgcagca gaacctgagt tgatcaaatg gatgatgccg
    32221 tgttctgcca gtccatcgag tttatgcccc gttacacgct caattgcttc tggtgaccag
    32281 taggtacgca catcggcaaa tacctgagcg gtgccggtga gctggtgacc cattagcatt
    32341 gccacgccgt taagactgtc gttttcggtc gccacgacaa agggttcgcg cacgccattc
    32401 cagtcaaatg aactgttgag gatcgcttcg gcggtgtcac cattgggata ttgatcggtc
    32461 cagtgacgtt gcccctggaa gcccgcagcg atggcgttgt agccaagtga ttcttccacg
    32521 cgaccaatat cggccagttt gctgttgcct tgcatcatgt cgcggataca catcgccatc
    32581 agtaaacttt cgcgcagaac tgcgcggctt tgttcggcat tgcgttgata ctgtttgtta
    32641 ttttcatctt cgccatagcg gaagttttta tcagcccagg ccagtgccat ttccagttcg
    32701 gcttcgtcgt aaatcttctg atcgatacgg cgacgcagtt cggtcatatc caccgcctgg
    32761 actttcattc ccagccagga ttcaaagaag ttgtgatcaa cgatggaacc ggcgataccc
    32821 atcgaaacgc cgcccagcga cagatagctt ttacctttca tgctggcgac ggccaaaccg
    32881 gcgcgggcaa agcgcagcag tttttcttca acatcggcag gaatcgatgt gtcatcggca
    32941 tcctgaacgt catgaccgta aatggagaat gccgggatgc ctttctggct gtgagctgcc
    33001 agagccgctg ccaggtaaac agcgccggga cgttcagtgc cgttaaagcc ccaaatggct
    33061 ttcgggcggg ttggatccat gtcgatggtt tcactgccat agcaccagca aggcgttacc
    33121 gtaatggtga ggcctacatt ctggctgctg aatttttctt cgcaagcagc ggcttcagcc
    33181 atacccgcga tacaggtatc ggaaatgaca cactcgacgg cagctccgca ggcatggcgc
    33241 agtttctcgg tcagcagtgc ggccgtagct ttcgccatat tcattgtttg ttcttcaagc
    33301 gactcacgaa cacccatgcg acgaccgtca ataaccgggc ggataccaat tttcggtaag
    33361 ctgatttttt tcattacaga ttcctcactt tattcggaaa atatgttcag ttagttgccg
    33421 tttgagaacg gaaacgggca aagataaaga tgaccgcgaa gcagagtgcg gggatcagtt
    33481 cagcagtggg gatgttgccc gccgcgtcac tgacaaaacc catgaccgga gtgacaatac
    33541 cgccgccaat aatggtcata acgatgaagg acgaaccgta tttggtgtcc tggccgagat
    33601 tcttaatgcc cagcgagaag attgttgggt actgaatcga cataaaggcg ctgcataaag
    33661 tcagggctat taagcccaca tgaccgccag cgaaggctga gatcaggcac agtgccatag
    33721 cgattaatgc gtaggcggcc aggactttgt gtggtgcgaa gcgactgatg agccaggtac
    33781 cggtgaaacg accaataaag aagcacacca tggttccggt taaatagtta gcggcaaagc
    33841 cggcagtcat acctggaatt tcttctacag cgtagcgaat caaatagctc cagcaggccg
    33901 tttgtgcgcc gacatagcag aattgcgcta ataccgccca gcgccagtga cgaatacgcg
    33961 ccagacgaga aagcgatgcg gagaacgatc cttgtttggc gtcactgtga ttatcactct
    34021 gcaatgccgg gaatttcgtc agcatgatca gcagggcgac cagtaacacg atagccacga
    34081 tgatcatata aggtgtctgt accgataata ccaggctgtg tttatacgca ctcaattgct
    34141 ctggagacat tttatcgaga acgtcttgcg attgatgtgg cacgttagac aaaataagac
    34201 tttgcccaaa gacaaccgcg ataattgcgc caaacgagtt aaatgtttgc gcaagattta
    34261 agcggaagtg accactgctt tctggcccta ataccgtaac aaaagggttt gcggcagttt
    34321 ccagacaacc taatccggct gcaataataa ataggccaac taaaaataag gtgtagttca
    34381 ttatttctgc agcgggccag aataatgcag cgcccaaggc atataaaaat aatccggtaa
    34441 taatccctgc tttataactg agttttttca tcaatatccc agcagggatt gggataatga
    34501 aataaccaaa gtaaaaggcc gattggatca ggccagcctg gaaatttgtc agcgtaaaag
    34561 cctgctggaa ttgaggtaat aaaatgtcgt taaggttatt ggctaccgcc caaagaaaaa
    34621 acagtgagca cagcagcgcg aatggaataa tgtaacttct gctttgccct gcatctttat
    34681 ctaccgcacg gtaactctgc gtttgtattg atgtgtttcc catagcatcc tcttaggttc
    34741 agaagcttaa gcacgtaatc ggttatgttt tcaaaagtgc aaaaccagcg agttcgggct
    34801 tcatatcagt gaatatttgc ctgtctgaaa tgaactcatg gattcatttt caaataaaaa
    34861 acgctctgta atatgacggc ggtcacactt attagctgga aggtgacttt atgtgactac
    34921 catcacttta ataatcgaaa tttcatgccc gtttcagaaa ataaatcata aaaaacacaa
    34981 accagatgac cgtattaatt gtttatgccg aaaacgggca ttcctggctt gggtgaatag
    35041 aaaaatgacc gttttgataa atatttttaa gagcggtcat ttttaatggg gttataaaaa
    35101 gagaacttgc agttatttac tgtgattagt tgaaccaggt cacaaaatat aagcttgtga
    35161 atttctttct ggttaatcag gaagaagatg gtgggtaatt aaacggctaa ttcaatagtg
    35221 tgaaaggaac aacattattg ccctgttttg aatcagagag aggtagctaa tggaacgaaa
    35281 taaacttgct cgtcagatta ttgacacttg cctggaaatg acccgcctgg gactgaacca
    35341 ggggacagcg gggaacgtca gtgtacgtta tcaggatggg atgctgatta cgcctacagg
    35401 cattccatat gaaaaactga cggagtcgca tattgtcttt attgatggca acggtaaaca
    35461 tgaggaagga aagctcccct caagcgaatg gcgtttccat atggcggcct atcaaagcag
    35521 accggatgcc aacgcggttg ttcacaatca tgccgttcat tgcacagcag tttccattct
    35581 taaccgaccg atccccgcta ttcactacat gattgcggcg gcaggtggta attctattcc
    35641 ttgcgcgcct tatgcgacct ttggaacacg cgaactttct gaacatgttg cgctggctct
    35701 caaaaatcgt aaggcaactt tgttacaaca tcatgggctt atcgcttgtg aggtgaatct
    35761 ggaaaaagcg ttatggctgg cgcatgaagt tgaagtgctg gcgcaacttt acctgacgac
    35821 cctggcgatt acggacccgg tgccagtgct gagcgatgaa gagattgccg tagtgctgga
    35881 gaaattcaaa acctatgggt tacggattga agagtaattt cgtaaagcaa caaggagaag
    35941 gatgatggct aacagaatga ttctgaacga aacggcatgg tttggtcggg gtgctgttgg
    36001 ggctttaacc gatgaggtga aacgccgtgg ttatcagaag gcgctgatcg tcaccgataa
    36061 aacgctggtg caatgcggcg tggtggcgaa agtgaccgat aagatggatg ctgcagggct
    36121 ggcatgggcg atttacgacg gcgtagtgcc caacccaaca attactgtcg tcaaagaagg
    36181 gctcggtgta ttccagaata gcggcgcgga ttacctgatc gctattggtg gtggttctcc
    36241 acaggatact tgtaaagcga ttggcattat cagcaacaac ccggagtttg ccgatgtgcg
    36301 tagcctggaa ggactttccc cgaccaataa acccagtgta ccgattctgg caatccccac
    36361 cacagcaggc actgcggcag aagtgaccat taactacgtg atcactgacg aagaaaaacg
    36421 gcgcaagttt gtttgcgttg atccgcatga tatcccgcag gtggcgttta ttgacgctga
    36481 catgatggat ggtatgcctc cagcgctgaa agctgcgacg ggtgtcgatg cgctcactca
    36541 tgctattgag gggtatatta cccgtggcgc gtgggcgcta accgatgcac tgcacattaa
    36601 agcgattgaa atcattgctg gggcgctgcg aggatcggtt gctggtgata aggatgccgg
    36661 agaagaaatg gcgctcgggc agtatgttgc gggtatgggc ttctcgaatg ttgggttagg
    36721 gttggtgcat ggtatggcgc atccactggg cgcgttttat aacactccac acggtgttgc
    36781 aaacgccatc ctgctaccgc atgtcatgcg ctataacgct gactttaccg gtgagaagta
    36841 ccgcgatatc gcgcgcgtta tgggcgtgaa agtggaaggt atgagcctgg aagaggcgcg
    36901 taatgccgct gttgaagcgg tgtttgctct caaccgtgat gtcggtattc cgccacattt
    36961 gcgtgatgtt ggggtacgca aggaagacat tccggcactg gcgcaggcgg cactgaatga
    37021 tgtttgtacc ggtggcaacc cgcgtgaagc aacgcttgag gatattgtag agctttacca
    37081 taccgcctgg taaatgcgct gatgtgataa tgccggatac gacgtttgcg gcgtatccgg
    37141 ctcgccgtta ccgtaccaac cgcaattgct gtaaattgcc gtcgatatgc aaatcggttt
    37201 gtaagcgggc aatatcgcgg cacagaaacg ccatctcttt atgggtttct aattttttgc
    37261 gccacttttc ggcaaccgca tccagatttt catatatccc ttccagactc tgaaactcga
    37321 ccagcagctg cgtggcgctt tttggtccga ttcccgcaac acccggtacc tttgaactgc
    37381 tgatccccgc aagtccccag taatcgggca actgctgcgg ttgaacgcca aattctttat
    37441 cgataaatgg cgcatccagc caacgtttct ggaagtaatc acgaatacgt aatgtcggtg
    37501 aaagtaactg acagtagcct ttatctgtcg aaacaatcgt tgcctgatgc ccggcctgtg
    37561 tcactttgac cgccagcgtg gcggctaagt catcggcttc gttgccgctg gttgaccagc
    37621 acgggacgcc gcgttgctca aaggcggcgc gtaatgcagg catctcgtcg tgcaactctt
    37681 ccggcattgg cggtcgaccc gctttgtaat ctggtaaacg ctgatgacgc cagccgctac
    37741 tgcggttttc atcatcaaaa acggcgaccg cgtgggttgg ctggctgtgc ataatgagct
    37801 gatcgagcgc atgctggcag gtttcgacac agggcgatcc ctgaacggca tgaatgcgac
    37861 gaataagatt cagtgcatcg acaataagca aatgaacagc cacgataacc cccttaaaga
    37921 tcaggcgata agggtaacat tcgatgccgg aacaggctac ggctggatga gaaatcggga
    37981 agaggcctcg caaaacgagg cctctggaga gcgattaatc gcaggcaacg atcttcattg
    38041 ccaggccgcc gcgagaggtt tcgcggtact tggcgttcat atctttacct gtttcgtaca
    38101 tggtttcgat aactttatcg aggcagacgc gcggctcgct ggtacggcgt agcgccatac
    38161 gtgcggcgtt caccgctttt actgccgcaa tggcgttacg ctcgatgcat ggcacctgga
    38221 cctgtccggc gaccgggtca cacgtcagac cgaggttgtg ctccatggcg atttccgccg
    38281 cgatgcacac ctgcgccggg cttgcaccta acagttctgc cagaccagcc gccgccattg
    38341 agcacgccac accaacttca ccctggcagc cgacttcagc accagaaatt gacgcgttca
    38401 tcttataaag agaaccaatg gcactggcta ccagcaggta acgagccagt gagttagcgt
    38461 tcacttcgcg gataaacttg tcgtagtacg ccagcactgc cgggataatc ccgcacgcac
    38521 cgttagtcgg cgcagtcacc acgcgtccgc cagcagcgtt ctcttcgttc actgccagtg
    38581 caaacatgtt gatccagtca acaaccgcca tcgggtcagt ggtggtttta tcctggctga
    38641 ccagcatccg gcgtagtgcc gcagcacggc gtggaacgcg cagtttgcca ggcaacacgc
    38701 cttcggtgga aataccgcgc tcaataccgc cacgcatgac ttcccatacg ttcgccaggt
    38761 gctgttccag ctcttctttg ctgtgcagcg ccagctcatt tttcatcatc aggccagaga
    38821 gtgacagccc ggtttcctga caatgttttt gcagatcggc tgctgaactg tacggataag
    38881 gaacttcaac cggtgcgcta ttctgctggc caaaatgctc ttcatcaacg ataaagccac
    38941 cgccaataga gtagtaagtc tggctgtaaa cgactttatc gcccgccagc gcggtaatgc
    39001 gcataccgtt ttcatgcaga gaaaggttgt ctgcatggaa gttcatgcac tgatcaaccg
    39061 ggaactccac ttcatgctga ccgtttgcca gcatcaggcg accatgagta ttcacatcct
    39121 gaataaaacc agggatggaa tcgatatcca cggtatccgg caggttaccc gccaggccca
    39181 taataatggc gatatcagtg tggtggcctt taccggtcag agagagcgag ccgtacacgt
    39241 caaccaccac gcgggtcacg tctttaagca gattacgggc aatcagatcg tcggtaaatt
    39301 gtttacccgc tttcattggt ccaacggtat gagaactgga agggccaatg ccgattttga
    39361 aaatatcgaa tacgctaatc ataggaaata catcgcgtta aaacggagga agcgccgccc
    39421 gaaagcggcg cgaaaggact tagctgaaca gagagtagaa gattgcggag attgcaatca
    39481 gacccatcac gacaacgaat acgttgctga tgtgaccgct gtacttacgc attgccggta
    39541 ctttctgaat tgcgtacatc ggcatcagga acaggatcat cgcgatgatt gggccgccca
    39601 gggtttcaat catacccagg atgctcgggt tcagggtggc aacaatccag gtcgttacca
    39661 gcatgaacag cgcagtgata cggttcagct tgttgatttc gatagactta cctttaccac
    39721 gcagagattt aatcaccata ccgttgaagc cttcacgtgc gcccaggtag tggccgagga
    39781 aggatttggt gatagcgata atcgcgataa tcggagccat ccacgcgata accggtgcgt
    39841 taaagtggtt agccaggtaa gacagaatcg agatgttctg ctctttagcc gcagccaggt
    39901 ctgccggagt caggctcagt acacagctga agacgaagaa cattacggtc agcaccatca
    39961 tgatgtgtgc gaatgccagg atcttcgagc atttctgttc tgccatatcg ccgtactctt
    40021 cacgcttcgc aacggcgaaa gaagagatga tcggagagtg gttgaacgag aacaccatta
    40081 ccggaattgc cagccacagg gtcatccaca gaccgtttcc ggttgcagat gcagtgtcca
    40141 gagacagcgt ttccagtgct gcgccgttcc actgcgggat caggtacaga gccagcagca
    40201 tcaatacgcc aacaaacggg aacaccagaa tactcatcgc tttaacgatc atctgctcac
    40261 cgaagcgaac gatggtcatc atacccacga tcaggatcag cgacagaatc gcacgcggcg
    40321 gtggcgtcat acccagctgg tgagacatga agctttcaac tgtattggtg attgccacgc
    40381 tataaaccag caggatcggg tagatagcga agaagtagag cagggtaatc agtttacctg
    40441 cgccaatacc aaagtgttct tctacaacct cggtgatgtc ttcgcccggg tttttaccag
    40501 acagtacgaa gcgagtcagg ccgcggtgag caaaaaacgt catcgggaac gcaaggatag
    40561 ccatgatgat cagcgggatc ataccgccaa caccggcgtt gattggcagg aacagcacgc
    40621 ccgcgccgat tgccgtgccg taaaggccca gcatccacat ggtgtctgtc ttgcgccagg
    40681 cactacgaga gtctttcgac gcaatcgtgc tggtttgagt cgtttccatc tatttctcct
    40741 ggaggaatgc aaataattca gcttttagcc agttcagccc gggaaggtgc ctgaccgtaa
    40801 aaatgaaatc tgtctatcgg cgatggctta atcatttacc gccgtaataa tttgagggcg
    40861 gaaagataca cacaagttat gtatctatca gtgatcttga tcccaaatac aataatctac
    40921 ttggaagata gattatccca acgctttact ctgtaaatta aaagtaaaag agaatttaag
    40981 ggcaatattt gcccattaaa cgacatctga cttcttccgg ggcgcgcaat atatgtaggt
    41041 tgaactgaaa aaatcaatac ttttagttaa tcatggcgat aatttctttt tcatagcgat
    41101 aaaatacggt ttatgaaaat agggaaacgt ttgcgttgca acataaattg tatgacatat
    41161 taactgtgat gatatttgtc gcgtgagggt ggtaagaaac attcatataa caaatgaaat
    41221 gagttgatga gtaagtgacc aaaaacgaga aaacccggca acaaacgtca ccgggggagg
    41281 ggagattacg tgcagatttc gtagcaaggg atgtaggctg agcctggcaa cttcatacga
    41341 tgctgggcta caaaaccctg tagcaggtcg tccatacgac gcataatctc tttatcgccg
    41401 ttgattttgt aaggaccaaa ctcttcaatg gcgcgaatac cgacttcttt tacgttaccc
    41461 gccacaatac cggagaacgc acggcgcagg tcggcagcca gcacttcaac aggttgatcc
    41521 gggtaaagct tcagattagc catattctcg tgagacggct caaacggcat ttgcaaatct
    41581 ggcgcaatgc gcattgacca gttaaagctg taggcatcgc ctgtatcacg gcgattttct
    41641 ttcaccagcg gcatcgattt tttcatctga cgagcgactt cagcggcgtc atcaatgatg
    41701 atgcggtaat ggcggcgcgc gttttcaccc agcgtatgca cgacaaactc gtccagtacg
    41761 cggaagtagt cggcgctctc tttcgggccg gtgaggatca atggtaaaac ctgatctttg
    41821 ttggccgggt tcattaaaat tcccagcaaa tacagcaact cttctgccgt acccacaccg
    41881 ccagggaaga taatgatacc gtgagcgata cggacaaacg cttccagacg tttttcgata
    41941 tctggcatga tgatcaattc gttgaccagc gggttaggcg gttcagcggc gataatcgac
    42001 ggctctgtca taccaataaa acgactgtct ttgtaacgct gctgcgcgtg tccgaccgca
    42061 gcacctttca tcggcgcttc catcgctccc ggaccacagc cggtgcagat attcagctca
    42121 cgcaggccca gctggtttcc gacgcgacgg gcatacaaat actcgttttc gttaattgag
    42181 tgaccgcccc agcagaccac catatttggc gcttcaccca catgcagcgc gcgagcgtta
    42241 cgcaagatgg aaaagaccag gttagtgatg tggacggagt tgtcgagatt caggttggga
    42301 aaacgaacgg tgttatggat ttgcccgtaa acgaagagaa tgtcacgcag gaccgcgaac
    42361 aagttggcct gcaaagcgcg aataattcgc ccatcgacaa aagcctcttc cgggggatta
    42421 atcagttcca gctttacgcc gcgttcacgg cgcaagacgt taatatcgaa attttcaaaa
    42481 cgagacagca attctttgct gttatcggtc aaactaccgg agttcagtac ggcaagtgaa
    42541 cagttgcgaa acagttgata gaggtcgctg ctggcggtgc gtttaagcat atccacttcc
    42601 agctgcgaca acatatccat ggagccaagc gggctaatat gtgtaatcaa gaaaaactcc
    42661 ttatgggacg aatatcgtca ttccctttga ttacaatagc cctgcctgat gacctttcac
    42721 aaccttctgc gtgaatccag cacgcaaaat tgagaaaaaa tttattgccg aaccagtctt
    42781 gtggtcgatg ggacaaaatc gctattactg cgccacgggt taatgtccag accgccacga
    42841 cgggtataac gtgcgtaaac gctcaatttc tctggctggc agaagcgtaa caggtcatta
    42901 aagatgcgtt ccacgcactg ttcgtggaac tcgttgtgat gacggaatga gaccaggtaa
    42961 cgcagcagtt tttctctgtc gatttggcgt ccacgatact gaatttggat cgatccccaa
    43021 tctggttgat gggtgatcag gcagtttgat ttcagcaggt ggctgacaag cgtctcttcc
    43081 actacttttt caccactggt ggcattctcc agatagtcag tagtgaattc atagttatcg
    43141 atagtgatat cctggtcatc aatgcaagtg ccattgaaat gacctatcgg ctggccttcc
    43201 agttcatcaa gacgatataa cgccacgcta accttaccct gagcgcaagt gcttaagtcg
    43261 cgctccagcg tctggcgcac ctcatcccag ttattaaaac gcgtctggtt aaaactgttg
    43321 agataaagct taaaactctt cgactcaatc agatttacgc tggtgtaatc aagttcaaca
    43381 tgaccgacag cgacctgcgg caaacctttc gcattcagcc aggaaagttc atacagcgtc
    43441 cagatatccg taccgtgaaa aggcaggtta tccgctttca gacccagcgg gtcgcgattc
    43501 aggctgcgtg gaacgccttg cagtaggctg gcgtcatagg tatcccggta atcggttgat
    43561 tttccaagag tcaggcccgc aagtgcctga tggtttgcat aagaagacat gtttcaccgt
    43621 cataaaccag ataaactaaa cgacaagtgt atctggttta agtgaagaga gaaattagtg
    43681 gacgatttga ccgcacaagc cctgaaagat tttactgcac gctactgcga tgcgtggcat
    43741 gaagagcata aaagctggcc gttaagtgag gaactgtacg gtgttccttc accgtgcatt
    43801 atttcaacta ccgaagatgc cgtatactgg caaccccagc cgtttaccgg ggaacaaaat
    43861 gtaaacgcgg ttgaacgcgc ttttgatatt gtgatacaac ccacaattca taccttttat
    43921 acgactcagt ttgccgggga tatgcacgcg cagtttggcg atatcaaact gacattgctg
    43981 caaacctgga gtgaagacga cttccgtcgg gtgcaggaaa atctgattgg tcatctggtg
    44041 actcagaaaa ggctaaaact gccgcccacg ctatttatcg cgacgctgga agaagagtta
    44101 gaggtcatct cggtgtgtaa tctctccggt gaagtgtgca aagagacgct gggaacacgt
    44161 aaacggacgc atttagcctc aaatcttgcg gaattcctta atcaacttaa gcctcttctg
    44221 taatccaaat accccatctg gttgtgagag atctcttaca gactctgtag gagatcgcca
    44281 ggaaataagc gaatacttaa aaagataaga atcgttattt tcatttaaaa tcaaaatgtt
    44341 gattgttaat tcttaacttt catatgaaat tttccttaag gcattgtcgg taagcgcctt
    44401 gtaagacttc gcgaaaaaga cgattctatc ttcgtcgaca gggagtcaga caacgaagtg
    44461 aacatcagga tgatgacact tctgcaggac acaccaggat ggtgtttcag ggaaaggctt
    44521 ctggatgaag cgaagaggat gacgcaggac gcgttaaagg acacctccag gatggagaat
    44581 gagaaccggt caggatgatt cggtgggtca ggaaggccag ggacacttca ggatgaagta
    44641 tcacatcggg gtggtgtgag caggaagcaa tagttcagga tgaacgattg gccgcaaggc
    44701 cagaggaaaa gttgtcaagg atgagcaggg agcaacaaaa gtagctggaa tgctgcgaaa
    44761 cgaaccggga gcgctgtgaa tacagtgctc ccttttttta ttcctgctat ccttcgcggc
    44821 agtttttctt attgaggttg ctttatgacc actcatgacc gcgttcgtct ccagttgcag
    44881 gcgcttgaag cgttactgcg tgaacatcag cactggcgaa acgatgaacc ccagccgcat
    44941 caatttaata gtacccaacc gttctttatg gacaccatgg aaccactgga gtggttgcag
    45001 tgggtgctga tcccgcgaat gcacgatttg ttagataaca aacagccgtt accgggcgct
    45061 tttgccgttg caccctatta tgaaatggcg ctggcgacgg accatccgca acgcgcgctt
    45121 attctggctg agctggaaaa actggatgct ctttttgcgg atgatgcgag ctaatgctgg
    45181 aaatactcta tcaggatgaa tggctggttg cggtaaataa accctccggc tggctggttc
    45241 accgcagctg gctggatcgc gacgagaaag tagtggtcat gcaaaccgtg cgtgaccaga
    45301 taggccagca tgtttttact gctcatcgtc tggaccgacc cacttctggt gtgttgttga
    45361 tgggattatc cagcgaggcc ggacggctgc tggcacaaca gtttgaacag caccaaatcc
    45421 agaaacgtta ccatgcgatt gtgcgcggct ggttgatgga agaagcggtg ctggattatc
    45481 cactggtgga agaactggac aaaatcgctg ataaatttgc ccgcgaagat aaaggcccgc
    45541 agccagcagt gacgcattat cgcggtctgg cgaccgtaga aatgccggta gcgaccggac
    45601 gttacccgac cacgcgctac ggcctggtgg aactggagcc gaaaaccgga cgcaaacatc
    45661 agcttcgccg acatcttgcc catttgcgtc atccgattat tggcgatagc aaacatggcg
    45721 atttacgcca gaatcgcagc ggtgctgagc attttggcct ccagcggtta atgctacatg
    45781 ccagtcagtt gtcattgacg catcctttta ctggcgagcc gctgactatt cacgcgggcc
    45841 tggacgacac ctggatgcag gcgttatcac aatttggctg gcgcggtctg cttcctgaaa
    45901 atgaaagggt tgagttcagt gcgccatcgg gtcaggatgg agagataagt tcctaatcca
    45961 gggagaaaac ggtatggcgg aaattggtat ttttgtcggc accatgtacg ggaattcact
    46021 gttagtggcc gaagaagcgg aagcgattct gaccgcgcag ggccacaaag caacggtatt
    46081 tgaagatcct gaattaagcg actggctgcc ctatcaggat aagtatgttc tggtggttac
    46141 gtccacgacc gggcagggcg accttcctga tagcattgtg ccactctttc agggaatcaa
    46201 agatagtctg ggcttccagc cgaatctgcg ttatggcgtg attgcgctcg gcgacagtag
    46261 ttatgtgaat ttctgcaatg gcggcaaaca gttcgatgcc ttgttgcagg aacagagcgc
    46321 tcagcgggtt ggcgaaatgc tgttgattga cgccagtgag aacccggaac cggaaacgga
    46381 atcaaatccg tgggtcgaac agtggggcac gctgttgtcc tgatatgttc agcgagcggt
    46441 aaatgtcgtt ttagcggtgc tgaatcgaat cttttcaggc aaatgccagt aaaaactgct
    46501 tcatagcgcg gatttttact ggcgtttgcc tggagtcaag cgatccattt catactcttc
    46561 tttatttctt cgttttaacc cttcctttct tgttcttgtt ttcatttccg tgaagtggat
    46621 tccaccgtcc agggctaatg ccaaaatcgg gcctcattga acgcattaat gttgtgttgt
    46681 tgcacggtga gccgctatgg cgcgcttttt atactgctat tgccagatat aaacacgcgc
    46741 cgtattcggc gaacgaccta taaaaacggc aaaaaacacc ctacgtcacc tctgatttcc
    46801 tggcgatgtc gcagtccaga gtgagcgtgg ctaacgcgaa ttttcaggag tgcaacaatg
    46861 agttctttaa gtcaggctgc gagcagtgtg gaaaaacgca caaatgctcg ttactggata
    46921 gtggtgatgt tgtttatcgt cacatccttc aactacggcg accgcgctac gctctctatc
    46981 gccggttcgg aaatggccaa agatatcggc cttgatcccg tgggaatggg ctatgtgttc
    47041 tctgctttct catgggctta tgttatcggg cagatccctg gtggctggtt gctggaccgt
    47101 tttggttcaa aacgcgtcta cttctggtcg atctttatct ggtcgatgtt taccttgctg
    47161 caaggcttcg tcgatatctt tagtggattc ggcattatcg ttgccctgtt tacgctgcgc
    47221 ttcctggtcg ggcttgctga agcgccatct ttccccggca acagtcgcat tgttgcggcc
    47281 tggtttccgg cgcaggaaag gggaacggcg gtgtcgattt ttaactccgc tcaatacttc
    47341 gcaacggtga tcttcgcgcc gattatgggc tggctgacgc atgaagtggg ctggtcacac
    47401 gtcttcttct ttatgggcgg tctggggatt gtcatcagct ttatctggtt gaaagtcatc
    47461 cacgagccaa atcaacatcc gggggtaaat aagaaagagc tggagtacat cgccgcgggt
    47521 ggcgcgctga tcaatatgga tcagcaaaac accaaagtta aagtgccgtt cagcgtgaag
    47581 tgggggcaga tcaaacagct gctagggtca cggatgatga tcggcgttta tatcggtcag
    47641 tactgtatca acgccctgac ttacttcttt attacctggt tcccggttta tctggtgcag
    47701 gcacgcggaa tgtcgattct gaaagcgggc tttgtggctt ccgttccggc ggtttgcggt
    47761 tttatcggcg gtgtgctggg tgggattatt tccgactggc tgatgcgccg cacgggatcg
    47821 ctgaacattg cgcgtaaaac accgatcgta atgggcatgt tgctgtcgat ggtgatggtg
    47881 ttctgcaact acgtcaacgt tgagtggatg atcatcggct ttatggcgct ggccttcttc
    47941 ggtaagggca tcggggcgct gggttgggca gtaatggcag ataccgcgcc aaaagagatc
    48001 agcggtcttt ccggtggcct gttcaacatg ttcggtaaca tttctggcat cgtcacgcca
    48061 atcgcaattg gttatatcgt tggcacgact ggctcgttta atggggcgct gatttatgtt
    48121 ggtgttcatg ccttaatcgc ggtactgagc tacctggtgc tggtgggcga tatcaagcgt
    48181 atcgagttga aacctgttgc ggggcaataa gatggcgaca caatccagtc ctgttattac
    48241 tgatatgaaa gtcattccgg tggccgggca tgacagcatg ttgcttaata ttggtggggc
    48301 acataacgca tatttcaccc gcaatattgt ggtactcacc gataacgccg ggcataccgg
    48361 cattggtgaa gcgccgggcg gagatgtgat ttatcagacg cttgtcgatg ctattccgat
    48421 ggttctgggc caggaagttg cgcgactgaa taaagtggtc cagcaggtgc ataaaggtaa
    48481 tcaggccgcc gattttgata ccttcggtaa aggtgcctgg acctttgaat tacgcgttaa
    48541 cgccgtggcg gcgctggaag ccgctttgct tgacctgcta ggtaaggcgc tgaatgttcc
    48601 ggtctgcgaa ctgttagggc caggcaagca acgcgaggct attaccgtcc tcggttatct
    48661 gttttatatc ggtgatcgga ccaaaaccga tcttccttat gtggaaaata cgccgggcaa
    48721 ccatgagtgg tatcagttgc ggcatcagaa agcaatgaac agcgaagccg ttgtgcgtct
    48781 ggcggaagcc tcacaggatc gctacggctt taaagatttc aaacttaagg gcggcgtgtt
    48841 acctggcgag caagaaatcg acactgttcg tgcattgaag aaacgcttcc cggatgcgcg
    48901 gattaccgtt gatcccaacg gtgcatggct gcttgatgaa gccatttctt tatgcaaagg
    48961 gctgaatgat gttcttacct atgccgaaga tccatgcggc gcagaacagg gcttctccgg
    49021 acgtgaagtg atggcggaat ttcgacgggc gaccggcttg cccgtcgcga ctaacatgat
    49081 cgccaccaac tggcgcgaaa tgggtcatgc ggtgatgctc aatgcggtag atattccact
    49141 tgccgatccg cacttctgga cgctttccgg tgcagtccgt gtggcgcagc tttgcgacga
    49201 ctgggggctg acctggggtt gccattctaa taaccatttc gatatctctc tggcgatgtt
    49261 tacccatgtg ggcgcggcgg caccgggtaa tcctaccgct atcgataccc actggatttg
    49321 gcaggagggc gattgtcgcc tgacccaaaa tccgctggag attaaaaacg gaaaaattgc
    49381 cgttcctgat gcgcccggtc tgggcgtgga actggactgg gaacaggtac aaaaggcaca
    49441 tgaggcctat aaacgtctgc ctggcggtgc gcgtaacgac gcaggtccga tgcagtacct
    49501 gatccccggc tggacctttg accgtaaacg tcccgttttc ggccgtcatt gattctgaaa
    49561 aaggacataa atatgagttc tcaatttacg acgcctgttg ttactgaaat gcaggttatc
    49621 ccggtggcgg gtcatgacag tatgctgatg aatctgagtg gtgcacacgc accgttcttt
    49681 acgcgtaata ttgtgattat caaagataat tctggtcaca ctggcgtagg ggaaattccc
    49741 ggcggcgaga aaatccgtaa aacgctggaa gatgcgattc cgctggtggt aggtaaaacg
    49801 ctgggtgaat acaaaaacgt tctgacgctg gtgcgtaata cttttgccga tcgtgatgct
    49861 ggtgggcgcg gtttgcagac atttgaccta cgtaccacta ttcatgtagt taccgggata
    49921 gaagcggcaa tgctggatct gctggggcag catctggggg taaacgtggc atcgctgctg
    49981 ggcgatggtc aacagcgtag cgaagtcgaa atgctcggtt atctgttctt cgtcggtaat
    50041 cgcaaagcca cgccgctgcc gtatcaaagc cagccggatg actcatgcga ctggtatcgc
    50101 ctgcgtcatg aagaagcgat gacgccggat gcggtggtgc gcctggcgga agcggcatat
    50161 gaaaaatatg gcttcaacga tttcaaactg aagggcggtg tactggccgg ggaagaagag
    50221 gccgagtcta ttgtggcact ggcgcaacgc ttcccgcagg cgcgtattac gctcgatcct
    50281 aacggtgcct ggtcgctgaa cgaagcgatt aaaatcggta aatacctgaa aggttcgctg
    50341 gcttatgcag aagatccgtg tggtgcggag caaggtttct ccgggcgtga agtgatggca
    50401 gagttccgtc gcgcgacagg tctgccgact gcaaccaata tgatcgccac cgactggcgg
    50461 caaatgggcc atacgctctc cctgcaatcc gttgatatcc cgctggcgga tccgcatttc
    50521 tggacaatgc aaggttcggt acgtgtggcg caaatgtgcc atgaatttgg cctgacctgg
    50581 ggttcacact ctaacaacca cttcgatatt tccctggcga tgtttaccca tgttgccgcc
    50641 gctgcaccgg gtaaaattac tgctattgat acgcactgga tttggcagga aggcaatcag
    50701 cgcctgacca aagaaccgtt tgagatcaaa ggcgggctgg tacaggtgcc agaaaaaccg
    50761 gggctgggtg tagaaatcga tatggatcaa gtgatgaaag cccatgagct gtatcagaaa
    50821 cacgggcttg gcgcgcgtga cgatgcgatg ggaatgcagt atctgattcc tggctggacg
    50881 ttcgataaca agcgcccgtg catggtgcgt taagacagaa aaccaaaacc ggctctatct
    50941 ggagccggtt tgagactgat gctaaacgca aaatagcctg ttgtgctatg tatcaggtct
    51001 acatcatttt ttaattcatt gaatttaatt ggttttgtag gccggataag gcgttaacgc
    51061 cgcatccggc ataaacacag gcactttgtc accaatctga aaccagcgtc ataaaaagcc
    51121 gattgctact cgacaagaca tccattaccc gagaattttg ctggcttcgc gcgcgacatt
    51181 atccatctcg tccaacagtt ccagcagctc cggttccaaa tcttcttctt tagtaccgct
    51241 acgtagctgt tgttcgatca gttggcagag attcttcata cgtggcacac cgctatagcc
    51301 gcaactgcca tgcagtttat gaatcaaatc aaccaggcct tccgggtttt ctccaaccag
    51361 ctgttcctca actttgttgc gaacttcagg caggaaatcg agtaacattt gcagcatatc
    51421 gcgcgctaaa tcggtttttc ctgctgcctg gcgtagtgcc agttgccagt cgagggtcgc
    51481 attcgggttc accacaattt cgttgacttc gggcgtcacg acgcgagagg aaataccgct
    51541 gccaggcttg tagcgcaaca acaaattatg caatcgctct tcttcaatcg gtttcgccag
    51601 ataatcgctc atccctgcgc caagcagctt ctctttttgc ccggccattg catgcgccgt
    51661 taccgcgata accggcgttt gttgctgatg cgggagttgg tggatgagtt cgcaggcccg
    51721 aatgccatcc atgtccggca tttgaatatc cattaagatc aaatcgaacg gcatctgttt
    51781 cgcccgttca accgcctgat gcccgctatc gcaaagttcc acatgttgca ccatatcttc
    51841 cagcaatgcg ccgataagtt tcaggttagc ggggttgtca tcaaccgcca tgactgtcat
    51901 tgccagctta ctttcatcgg ttacaggcaa aagcgtgttt tgtttgtgat gacaaaattc
    51961 cgtcagggca ggcaacaggc gtgtaggtgt taatggtttc agcagacacg cgccgatacc
    52021 atcttgcttg agtttttcag cattgacttg tgcatggcaa ggaagtgcca gcatcaggaa
    52081 atcggtcatc gataccgctt tcgctaatcg ctcatgttgc attgttagcg gctcgcggaa
    52141 ggtcaccgcg atgcctaaca acatcatgtc gtaatgcgcg ggaggcagcg cggagaacgt
    52201 tgggctataa accacttcca gcggcgtttc acttaaaata tccagcgtgc attgcgctgc
    52261 tgcggagttt ggttcgacat aggccaggcg cttacctgcg aggcactggg tggatggccc
    52321 ttcgataata atgttcgggt tcagatcgag attaatgtgg aaccagaaag ttgaaccgcg
    52381 attcggctgg ctatggaacg aaatatcgcc gcccatttca ttaaccagtt tttgtgtaat
    52441 caccagcccc agcccggtgc caccatgacg gcgggaaata ctggcatcag cctgtcggaa
    52501 ggcctggaat aagcgcgatt gatcgcgttc aggaatacca atgccggtat cccgaatctg
    52561 cacttcaatc tgcactttgg tattactcag cgcacgtttt tctaccagaa tatcaatgtt
    52621 gccattctcg gtgaatttaa ttgcattccc caccaggtta gtgatgattt gctgtaatcg
    52681 cagcgggtcg ccgatcacgt tatcaggcac gtcgctttta atattgagcg tcagttctaa
    52741 ccctttatcg tgagaagaat gtgccagcag agtaacggct tcatccagcg tgctgcgtag
    52801 tgggaatgga atactttcca gaatcagctt acctgcttcc agtttcgaga agtcgagaac
    52861 atcattaata attgccagca aattatttgc cgaacgttca atcgtattca ggtgatcgcg
    52921 ctgcgttggt gttaattctg ttttcagcgt caggcgggta aagccaataa caccattcag
    52981 tggtgtacgc agctcgtgtg acatatttgc cagaaactcg gatttaatac gcgccgcttc
    53041 ctgggcgcgc tttttcgcca gatctaactc aacgttctga atttccatct gctccagcgt
    53101 ttcacgcaga tcggacgtcg cctggtcgat attgtgctgc atctcttcgt gataagcagc
    53161 cagcgacatt gccatcgagt tgataccgtt tttcagcata tccagctcgc cgagcataaa
    53221 tccttccacc cggctgtcga gttgcccgcg acggatgcgg tcgacggtat tcaccatgtt
    53281 gcgaatcgga ccggttacat cgcgcattaa gcgccagcca aaaataagcg caataccgat
    53341 acaaaacagc atcatcacgc tggaaataaa gatctcttta tattgctgca agcgaaccga
    53401 tttaagatcc agctccagcg caatatatcc caacatattt tgactatttt tggcatcgct
    53461 actgggcgat tcgtcggggg agtaactctc agaaataatc ggcgtgcgga ggatcataat
    53521 atcgccgtca cgagtgacag tgagctggcg aggaaacggc acgttgctgc cgagctgcat
    53581 tgatgaggga tcaagatgaa aattggaggt gacaaagagt cggttatttt catcataaac
    53641 cgaaatcgcg cgaacaatat cggaatggcg acgatgcagt acgcttatta actgaccgat
    53701 agattcgcga ttttgcaggc tcatgccata ttcagtagaa actgcaagcg gctcaataat
    53761 gctggcaccg gcatcttcca gttgacgctg caagtcgtta tagcgatgca cgacgaaaaa
    53821 gatactcagc aataaaccaa taaggacggt cggtgccagg atcagaatca tcatgcgtgc
    53881 gcgcaggctg tagttggtca tggagttccg ttatgggaca attaaggtca cactgttaaa
    53941 ttgagaaagt ctcgccaatg gcgcaattct actctgcaaa acgacgcacg acgacgcgtc
    54001 agatcataac cgtttcagtc aacgacctcg actcttttgg tcagggcgtg gcgcgacata
    54061 acggcaaaac gctatttatc cccggattat tgccgcagga aaacgcggaa gttactgtta
    54121 ctgaagataa aaaacagtat gcccgcgcta aagtcgtacg ccggttaagc gatagcccgg
    54181 aacgcgaaac gccacgctgt cctcattttg gcgtatgcgg tggctgtcag caacaacacg
    54241 ccagcgtgga tttacagcag cgaagcaaaa gtgcggcact cgcccgatta atgaaacacg
    54301 atgtctctga agtgatcgcc gatgttccct ggggctatcg ccgtcgcgcg cgtttaagtc
    54361 tgaactactt accgaaaaca cagcaacttc agatggggtt tcgcaaagcg ggctccagtg
    54421 acattgtcga cgtcaaacaa tgccccattt tagcgcccca acttgaagca ttgctgccca
    54481 aagtcagggc atgtctgggc agcttacaag ctatgcgcca tcttggtcat gttgaactgg
    54541 tacaggcaac cagcggcacg ctgatgattt tgcgccatac cgcaccgcta agttcggcag
    54601 atcgcgaaaa actggaacgc ttttcgcatt ctgaaggcct ggatctgtat ctcgcccccg
    54661 atagtgagat actcgaaacc gtctctggtg agatgccctg gtatgactca aacgggttgc
    54721 gcttaacttt tagcccgcgc gattttattc aggtcaatgc gggtgtgaac caaaaaatgg
    54781 tagcgcgtgc gttggaatgg ctggatgtgc aacctgaaga tcgcgtactg gatctgttct
    54841 gcggtatggg caactttaca ctgccattgg cgacacaagc tgccagtgtg gtcggtgtag
    54901 aaggtgttcc ggcgctggtg gaaaaaggcc agcagaatgc gcgtcttaat ggcttacaga
    54961 atgtgacgtt ttatcacgaa aatcttgaag aagatgtcac aaagcagccg tgggcgaaaa
    55021 acggcttcga taaagtgttg ctggacccgg cgcgagcagg tgccgcaggt gttatgcagc
    55081 aaattataaa actggaacct attcgtatag tttatgtatc ctgtaaccct gcaacgctgg
    55141 ctcgggatag cgaagcgtta ttaaaagcag gatataccat tgcgcgactg gcgatgctgg
    55201 atatgttccc acacacggga catctggaat cgatggtact tttctcgcgc gttaaatagt
    55261 tgcgatttgc cgatttcggc aggtctggtc cctaaaggag aggacgatgg ttgcggtaag
    55321 aagtgcacat atcaataagg ctggtgaatt tgatccggaa aaatggatcg caagtctggg
    55381 tattaccagc cagaagtcgt gtgagtgctt agccgaaacc tgggcgtatt gtctgcaaca
    55441 gacgcagggg catccggatg ccagtctgtt attgtggcgt ggtgttgaga tggtggagat
    55501 cctctcgaca ttaagtatgg acattgacac gctgcgggcg gcgctgcttt tccctctggc
    55561 ggatgccaac gtagtcagcg aagatgtgct gcgtgagagc gtcggtaagt cggtcgttaa
    55621 ccttattcac ggcgtgcgtg atatggcggc gatccgccag ctgaaagcga cgcacactga
    55681 ttctgtttcc tccgaacagg tcgataacgt tcgccggatg ttattggcga tggtcgatga
    55741 ttttcgctgc gtagtcatca aactggcgga gcgtattgct catctgcgcg aagtaaaaga
    55801 tgcgccggaa gatgaacgtg tactggcggc aaaagagtgt accaacatct acgcaccgct
    55861 ggctaaccgt ctcggaatcg gacaactgaa atgggaactg gaagattact gcttccgtta
    55921 cctccatcca accgaataca aacgaattgc caaactgctg catgaacggc gtctcgaccg
    55981 cgaacactac atcgaagagt tcgttggtca tctgcgcgct gagatgaaag ctgaaggcgt
    56041 taaagcggaa gtgtatggtc gtccgaaaca catctacagc atctggcgta aaatgcagaa
    56101 aaagaacctc gcctttgatg agctgtttga tgtgcgtgcg gtacgtattg tcgccgagcg
    56161 tttacaggat tgctatgccg cactggggat agtgcacact cactatcgcc acctgccgga
    56221 tgagtttgac gattacgtcg ctaacccgaa accaaacggt tatcagtcta ttcataccgt
    56281 ggttctgggg ccgggtggaa aaaccgttga gatccaaatc cgcaccaaac agatgcatga
    56341 agatgcagag ttgggtgttg ctgcgcactg gaaatataaa gagggcgcgg ctgctggcgg
    56401 cgcacgttcg ggacatgaag accggattgc ctggctgcgt aaactgattg cgtggcagga
    56461 agagatggct gattccggcg aaatgctcga cgaagtacgt agtcaggtct ttgacgaccg
    56521 ggtgtacgtc tttacgccga aaggtgatgt cgttgatttg cctgcgggat caacgccgct
    56581 ggacttcgct taccacatcc acagtgatgt cggacaccgc tgcatcgggg caaaaattgg
    56641 cgggcgcatt gtgccgttca cctaccagct gcagatgggc gaccagattg aaattatcac
    56701 ccagaaacag ccgaacccca gccgtgactg gttaaaccca aacctcggtt acgtcacaac
    56761 cagccgtggg cgttcgaaaa ttcacgcctg gttccgtaaa caggaccgtg acaaaaacat
    56821 tctggctggg cggcaaatcc ttgacgacga gctggaacat ctggggatca gcctgaaaga
    56881 agcagaaaaa catctgctgc cgcgttacaa cttcaatgat gtcgacgagt tgctggcggc
    56941 gattggtggc ggggatatcc gtctcaatca gatggtgaac ttcctgcaat cgcaatttaa
    57001 taagccgagt gccgaagagc aggacgccgc cgcgctgaag caacttcagc aaaaaagcta
    57061 cacgccgcaa aaccgcagta aagataacgg tcgcgtggta gtcgaaggtg ttggcaacct
    57121 gatgcaccac atcgcgcgct gctgccagcc gattcctgga gatgagattg tcggcttcat
    57181 tacccagggg cgcggtattt cagtacaccg cgccgattgc gaacaactgg cggaactgcg
    57241 ctcccatgcg ccagaacgca ttgttgacgc ggtatggggt gagagctact ccgccggata
    57301 ttcgctggtg gtccgcgtgg tagctaatga tcgtagtggg ttgttacgtg atatcacgac
    57361 cattctcgcc aacgagaagg tgaacgtgct tggcgttgcc agccgtagcg acaccaaaca
    57421 gcaactggcg accatcgaca tgaccattga gatttacaac ctgcaagtgc tggggcgcgt
    57481 gctgggtaaa ctcaatcagg tgccggatgt tatcgacgcg cgtcggttgc acgggagtta
    57541 ggccgaaatt tgctcgtatc tacaatgtag attgatatat actgtatcta catatgatag
    57601 cggtttgagg aaagggttat gatccacagt agcgtaaagc gttggggaaa ttcaccggcg
    57661 gtgcggatcc cggctacgtt aatgcaggcg ctcaatctga atattgatga tgaagtgaag
    57721 attgacctgg tggatggcaa attaattatt gagccagtgc gtaaagagcc cgtatttacg
    57781 cttgctgaac tggtcaacga catcacgccg gaaaacctcc acgagaatat cgactgggga
    57841 gagccgaaag ataaggaagt ctggtaatgg taagccgata cgtacccgat atgggcgatc
    57901 tgatttgggt tgattttgac ccgacaaaag gtagcgagca agctggacat cgtccagctg
    57961 ttgtcctgag tcctttcatg tacaacaaca aaacaggtat gtgtctgtgt gttccttgta
    58021 caacgcaatc aaaaggatat ccgttcgaag ttgttttatc cggtcaggaa cgtgatggcg
    58081 tagcgttagc tgatcaggta aaaagtatcg cctggcgggc aagaggagca acgaagaaag
    58141 gaacagttgc cccagaggaa ttacaactca ttaaagccaa aattaacgta ctgattgggt
    58201 agtgttacta actcacttct attctggtca caggtttcca cctgacagac ccgcaatttt
    58261 caggacaatt caatgaatca aatcgaccgt ttgctcacta ttatgcagcg cctgcgcgat
    58321 ccggaaaacg gctgcccgtg ggataaagag cagacatttg ccaccattgc gccttacacc
    58381 cttgaagaaa cctacgaagt gctggacgcc atcgcccgtg aagattttga cgatcttcgc
    58441 ggtgaactgg gcgatctgct attccaggtg gtgttttacg cgcaaatggc tcaggaagaa
    58501 gggcgctttg actttaatga tatttgcgct gctattagcg ataaattaga gcgtcgccat
    58561 ccgcatgttt ttgctgatag ttctgccgaa aacagtagtg aagtgcttgc ccgttgggag
    58621 caaatcaaaa ccgaagagcg cgcgcagaaa gcgcagcatt cggcgctgga cgatattcct
    58681 cgtagtttac cggctttaat gcgtgcgcaa aaaatccaga aacgttgcgc caacgttggc
    58741 ttcgattgga cgacgcttgg tccggtagtc gataaagtct acgaagagat cgacgaggtg
    58801 atgtacgaag cgcggcaggc tgttgtcgac caggctaaac tggaggagga aatgggggac
    58861 ctgctgtttg ccacggttaa tctggctcgc catttaggga cgaaagcaga aatcgcattg
    58921 caaaaagcga acgaaaaatt cgagcgtcgt tttcgcgaag tggagcgtat tgttgccgcg
    58981 cgtggactgg aaatgacagg tgttgacctc gaaacaatgg aagaagtctg gcaacaggta
    59041 aaacggcagg aaattgatct ctaagggaat tacgcggtca agcgcttgtc attttttaaa
    59101 tgacaagcgc ttgatttgcg tcaaaaacat ttaccccaaa ggggctattt tctcactcct
    59161 gatttcaata gtgcgctggc gaagaggagg gataatgaaa gtttgtggca caggtcatgt
    59221 tcgggtatac tgctttcccg tcctggttat tccatcgtct tttcaaccta acttctcagg
    59281 ttcagcatga caacgaacta tatttttgtg accggcgggg tcgtatcctc tctgggtaaa
    59341 ggcattgccg cagcctccct cgcagccatt cttgaagccc gtggcctcaa tgtgaccatc
    59401 atgaaactgg atccgtacat caacgtcgat ccaggtacta tgagcccaat ccaacacggg
    59461 gaagtgttcg ttactgaaga cggcgctgaa accgacctgg acctggggca ctacgagcgt
    59521 ttcattcgta ccaaaatgag ccgccgcaac aacttcacca cgggtcgtat ctactctgac
    59581 gttctgcgta aagaacgccg cggtgactac ctcggcgcaa ccgtgcaggt tattccgcac
    59641 atcactaacg caatcaaaga gcgcgtgctg gaaggtggcg aaggtcatga cgtagtactg
    59701 gtagaaatcg gcggtacagt aggtgatatc gaatccttgc cgttcctcga agcgattcgc
    59761 cagatggctg ttgaaattgg ccgtgagcac actctgttta tgcacctgac gctggtgccg
    59821 tacatggcag cgtctggtga agtcaaaacc aaaccgactc agcactctgt aaaagagctg
    59881 ctctccatcg gtatccagcc tgacatcctg atttgtcgtt cagatcgcgc tgttccggcg
    59941 aacgaacgtg cgaagattgc attgttctgt aatgttccgg aaaaagcggt tatttctctg
    60001 aaagacgtcg attccatcta taaaattccg ggcctgttga aatctcaggg gctggacgat
    60061 tatatttgta aacgattcag cttaaactgc ccggaagcga atctgtccga atgggaacag
    60121 gttatcttcg aagaagcgaa cccggtaagt gaagtcacca tcggtatggt cggcaagtac
    60181 attgaactgc cggatgctta taaatcagtg atcgaagcac tgaaacacgg tgggctgaag
    60241 aatcgtgtca gcgtcaacat caaactgatc gattcacaag atgttgaaac gcgcggcgtt
    60301 gaaatcctta aaggtctgga cgcaatcctc gtacctggcg gtttcggcta tcgtggcgta
    60361 gaaggcatga ttacgaccgc gcgttttgcg cgtgagaaca atattcctta tctgggcatt
    60421 tgcctgggta tgcaggtggc gttaattgat tacgctcgcc atgttgccaa catggagaac
    60481 gccaactcta cggaatttgt gccagactgt aagtacccgg ttgtggcgct gattaccgag
    60541 tggcgcgatg aaaacggcaa cgttgaagtt cgtagcgaga agagcgatct cggcggtacc
    60601 atgcgtctcg gcgcacagca gtgccagttg gttgacgata gcctggttcg ccagctgtac
    60661 aatgcgccga caattgttga gcgtcatcgt caccgttacg aagtcaacaa catgctgttg
    60721 aaacagattg aagatgcagg tctgcgcgtt gcgggccgtt ccggggatga tcagttggtc
    60781 gagatcatcg aagttccgaa tcacccgtgg ttcgtggctt gccagttcca tccggagttt
    60841 acttctactc cacgtgatgg tcacccgctg tttgcaggct ttgtgaaagc cgccagcgag
    60901 ttccagaaac gtcaggcgaa gtaagtaaaa aagttagagc ggcaacgcgt accctgggta
    60961 cgcgttgttt gtctggagtt tcagtttaac tagtgacttg aggaaaacct aatgtccaaa
    61021 atcgtaaaaa tcatcggtcg tgaaatcatc gactcccgtg gtaacccgac tgttgaagcc
    61081 gaagtacatc tggagggtgg tttcgtcggt atggcagctg ctccgtcagg tgcttctact
    61141 ggttcccgtg aagctctgga actgcgcgat ggcgacaaat cccgtttcct gggtaaaggc
    61201 gtaaccaaag ctgttgctgc ggtaaacggc ccgatcgctc aggcgctgat tggcaaagat
    61261 gctaaagatc aggctggcat tgacaagatc atgatcgacc tggacggcac cgaaaacaaa
    61321 tccaaattcg gcgcgaacgc aatcctggct gtatctctgg ctaacgccaa agctgctgca
    61381 gctgctaaag gtatgccgct gtacgagcac atcgctgaac tgaacggtac tccgggcaaa
    61441 tactctatgc cggttccgat gatgaacatc atcaacggtg gtgagcacgc tgacaacaac
    61501 gttgatatcc aggaattcat gattcagccg gttggcgcga aaactgtgaa agaagccatc
    61561 cgcatgggtt ctgaagtttt ccatcacctg gcaaaagttc tgaaagcgaa aggcatgaac
    61621 actgctgttg gtgacgaagg tggctatgcg ccgaacctgg gttccaacgc tgaagctctg
    61681 gctgttatcg ctgaagctgt taaagctgct ggttatgaac tgggcaaaga catcactttg
    61741 gcgatggact gcgcagcttc tgaattctac aaagatggta aatacgttct ggctggcgaa
    61801 ggcaacaaag cgttcacctc tgaagaattc actcacttcc tggaagaact gaccaaacag
    61861 tacccgatcg tttctatcga agacggtctg gacgaatctg actgggacgg tttcgcatac
    61921 cagaccaaag ttctgggcga caaaatccag ctggttggtg acgacctgtt cgtaaccaac
    61981 accaagatcc tgaaagaagg tatcgaaaaa ggtatcgcta actccatcct gatcaaattc
    62041 aaccagatcg gttctctgac cgaaactctg gctgcaatca agatggcgaa agatgctggc
    62101 tacactgcag ttatctctca ccgttctggc gaaactgaag acgctaccat cgctgacctg
    62161 gctgttggta ctgctgcagg ccagatcaaa actggttcta tgagccgttc tgaccgtgtt
    62221 gctaaataca accagctgat tcgtatcgaa gaagctctgg gcgaaaaagc accgtacaac
    62281 ggtcgtaaag agatcaaagg ccaggcataa gactgacttt atctgattta aaaatgccag
    62341 cctccgggct ggcatttttt atggttgagt taccagcggc cggatgcgcc gccacctcca
    62401 gaagaaccgc ccccgccgct aaatccgccg ctatctgagc ctccagcgga accgctgttg
    62461 tctgaatgat aacttctacc agatgctctt ttctgaagta cacataaaca ggtaaagacc
    62521 ataaatatgg tgaaagtaaa aaaaaaggaa aatacagcaa tggaaaaaat tttatcggag
    62581 aacaggaata aatctaaaag atagattgct gaaatacaga ctccactttt acatgcgcga
    62641 caaaaattac tgccttgatg aaaaaaccag aaagggaaga acatcactgc aaatacccaa
    62701 aaaatagcaa agtaataatg atcactggaa gacgctgatt cactttctga aggattagtc
    62761 ggatattggt gttgtgaagt gagttggtta ttcaaagcgt ttatagctag ctctaatccc
    62821 ttagctaatt tttgttgttt aaatgcgggt atcatgttgc tacggatgat atctccagcc
    62881 agagcatcgg ttaccttttc ttccagccca taacctacct ggatgcggac agtgcgatcc
    62941 gaccaggcaa caacgatcag tatcccatca ttacgtttgg catctcctaa acgccaattg
    63001 tcaaaaactc ttgtagcata ttgttcaatt gtctcgtctt tggttgtggg aacaattaat
    63061 acggcaattt gcacttgggt attattgaaa atagattcta acttgtgcgt cagttctttc
    63121 ttttcttgtg agtttaagct actcgtaaga tccgtaactt gttgctgtaa ttgtgggaca
    63181 atagttggtt gggctgcaac aaaggaacat acgaaagtga acattagtat gaaat
//
LOCUS       DAPHBJ010000007        52800 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.007, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000007 DAPHBJ010000000
VERSION     DAPHBJ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.007"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            77..640
                     /gene="srlA"
                     /locus_tag="RHZ76_000399"
                     /gene_synonym="gutA"
     CDS             77..640
                     /gene="srlA"
                     /locus_tag="RHZ76_000399"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDV2811101.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            637..1596
                     /gene="srlE"
                     /locus_tag="RHZ76_000400"
                     /gene_synonym="gutE"
     CDS             637..1596
                     /gene="srlE"
                     /locus_tag="RHZ76_000400"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDV2811102.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGIAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPHPSSVGRDYDTSKKITEQSDGLLAKVGMSMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            1607..1978
                     /gene="srlB"
                     /locus_tag="RHZ76_000401"
                     /gene_synonym="gutB"
     CDS             1607..1978
                     /gene="srlB"
                     /locus_tag="RHZ76_000401"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDV2811103.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            1982..2761
                     /gene="srlD"
                     /locus_tag="RHZ76_000402"
     CDS             1982..2761
                     /gene="srlD"
                     /locus_tag="RHZ76_000402"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDV2811104.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            2867..3226
                     /gene="gutM"
                     /locus_tag="RHZ76_000403"
     CDS             2867..3226
                     /gene="gutM"
                     /locus_tag="RHZ76_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDV2811105.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTIFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            3293..4066
                     /gene="srlR"
                     /locus_tag="RHZ76_000404"
                     /gene_synonym="gutR"
     CDS             3293..4066
                     /gene="srlR"
                     /locus_tag="RHZ76_000404"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HDV2811106.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            4059..5024
                     /gene="gutQ"
                     /locus_tag="RHZ76_000405"
     CDS             4059..5024
                     /gene="gutQ"
                     /locus_tag="RHZ76_000405"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HDV2811107.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(5021..6535)
                     /gene="norR"
                     /locus_tag="RHZ76_000406"
     CDS             complement(5021..6535)
                     /gene="norR"
                     /locus_tag="RHZ76_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HDV2811108.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            6722..8161
                     /gene="norV"
                     /locus_tag="RHZ76_000407"
     CDS             6722..8161
                     /gene="norV"
                     /locus_tag="RHZ76_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HDV2811109.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALKLCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            8158..9291
                     /gene="norW"
                     /locus_tag="RHZ76_000408"
     CDS             8158..9291
                     /gene="norW"
                     /locus_tag="RHZ76_000408"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HDV2811110.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGIQA
                     TLDRQRNIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNTDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(9419..11671)
                     /gene="hypF"
                     /locus_tag="RHZ76_000409"
     CDS             complement(9419..11671)
                     /gene="hypF"
                     /locus_tag="RHZ76_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HDV2811111.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETFLVQLYQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGKIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALAGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRFFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(11824..12351)
                     /gene="hydN"
                     /locus_tag="RHZ76_000410"
     CDS             complement(11824..12351)
                     /gene="hydN"
                     /locus_tag="RHZ76_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HDV2811112.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(12500..13510)
                     /gene="ascG"
                     /locus_tag="RHZ76_000411"
     CDS             complement(12500..13510)
                     /gene="ascG"
                     /locus_tag="RHZ76_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HDV2811113.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAP
                     SR"
     gene            13770..15227
                     /gene="ascF"
                     /locus_tag="RHZ76_000412"
     CDS             13770..15227
                     /gene="ascF"
                     /locus_tag="RHZ76_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HDV2811114.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDSITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKYQSVQPTVAKEVSLN"
     gene            15236..16660
                     /gene="ascB"
                     /locus_tag="RHZ76_000413"
     CDS             15236..16660
                     /gene="ascB"
                     /locus_tag="RHZ76_000413"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HDV2811115.1"
                     /translation="MSVFPESFLWGGALAANQSEGAFREGDKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDEFAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(16819..17289)
                     /gene="hycI"
                     /locus_tag="RHZ76_000414"
     CDS             complement(16819..17289)
                     /gene="hycI"
                     /locus_tag="RHZ76_000414"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HDV2811116.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(17282..17692)
                     /gene="hycH"
                     /locus_tag="RHZ76_000415"
     CDS             complement(17282..17692)
                     /gene="hycH"
                     /locus_tag="RHZ76_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HDV2811117.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(17689..18456)
                     /gene="hycG"
                     /locus_tag="RHZ76_000416"
     CDS             complement(17689..18456)
                     /gene="hycG"
                     /locus_tag="RHZ76_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HDV2811118.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(18456..18998)
                     /gene="hycF"
                     /locus_tag="RHZ76_000417"
     CDS             complement(18456..18998)
                     /gene="hycF"
                     /locus_tag="RHZ76_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HDV2811119.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(19008..20717)
                     /gene="hycE"
                     /locus_tag="RHZ76_000418"
     CDS             complement(19008..20717)
                     /gene="hycE"
                     /locus_tag="RHZ76_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HDV2811120.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWITVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(20735..21658)
                     /gene="hycD"
                     /locus_tag="RHZ76_000419"
     CDS             complement(20735..21658)
                     /gene="hycD"
                     /locus_tag="RHZ76_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HDV2811121.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(21661..23487)
                     /gene="hycC"
                     /locus_tag="RHZ76_000420"
     CDS             complement(21661..23487)
                     /gene="hycC"
                     /locus_tag="RHZ76_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HDV2811122.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAAVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(23484..24095)
                     /gene="hycB"
                     /locus_tag="RHZ76_000421"
     CDS             complement(23484..24095)
                     /gene="hycB"
                     /locus_tag="RHZ76_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HDV2811123.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(24220..24681)
                     /gene="hycA"
                     /locus_tag="RHZ76_000422"
     CDS             complement(24220..24681)
                     /gene="hycA"
                     /locus_tag="RHZ76_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HDV2811124.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            24893..25243
                     /gene="hypA"
                     /locus_tag="RHZ76_000423"
     CDS             24893..25243
                     /gene="hypA"
                     /locus_tag="RHZ76_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDV2811125.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            25247..26119
                     /gene="hypB"
                     /locus_tag="RHZ76_000424"
     CDS             25247..26119
                     /gene="hypB"
                     /locus_tag="RHZ76_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HDV2811126.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            26110..26382
                     /gene="hypC"
                     /locus_tag="RHZ76_000425"
     CDS             26110..26382
                     /gene="hypC"
                     /locus_tag="RHZ76_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HDV2811127.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGVLLYGEEK"
     gene            26382..27503
                     /gene="hypD"
                     /locus_tag="RHZ76_000426"
     CDS             26382..27503
                     /gene="hypD"
                     /locus_tag="RHZ76_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HDV2811128.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVQQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            27500..28510
                     /gene="hypE"
                     /locus_tag="RHZ76_000427"
     CDS             27500..28510
                     /gene="hypE"
                     /locus_tag="RHZ76_000427"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HDV2811129.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAVDKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            28584..30662
                     /gene="flhA"
                     /locus_tag="RHZ76_000428"
     CDS             28584..30662
                     /gene="flhA"
                     /locus_tag="RHZ76_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HDV2811130.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLDAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVALEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(30699..31052)
                     /locus_tag="RHZ76_000429"
     CDS             complement(30699..31052)
                     /locus_tag="RHZ76_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HDV2811131.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPHE"
     gene            complement(31129..31263)
                     /locus_tag="RHZ76_000430"
     CDS             complement(31129..31263)
                     /locus_tag="RHZ76_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2811132.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSVG
                     "
     gene            31339..33900
                     /gene="mutS"
                     /locus_tag="RHZ76_000431"
     CDS             31339..33900
                     /gene="mutS"
                     /locus_tag="RHZ76_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDV2811133.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAGLQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            34006..34662
                     /gene="pphB"
                     /locus_tag="RHZ76_000432"
     CDS             34006..34662
                     /gene="pphB"
                     /locus_tag="RHZ76_000432"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDV2811134.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFTPVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEIINDNIKYVIAHADYPGDEYQFGKEIVESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
                     "
     gene            complement(34713..35480)
                     /locus_tag="RHZ76_000433"
     CDS             complement(34713..35480)
                     /locus_tag="RHZ76_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV2811135.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            35676..36584
                     /locus_tag="RHZ76_000434"
     CDS             35676..36584
                     /locus_tag="RHZ76_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDV2811136.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIAIALAGFDLEMLDAPVSGGAVKAANGEMTIMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            36581..37843
                     /locus_tag="RHZ76_000435"
     CDS             36581..37843
                     /locus_tag="RHZ76_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HDV2811137.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQARKAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSIETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDDAFFSRAQREFLS"
     gene            37840..38478
                     /locus_tag="RHZ76_000436"
     CDS             37840..38478
                     /locus_tag="RHZ76_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDV2811138.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            38483..39259
                     /locus_tag="RHZ76_000437"
     CDS             38483..39259
                     /locus_tag="RHZ76_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDV2811139.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            39348..40712
                     /locus_tag="RHZ76_000438"
     CDS             39348..40712
                     /locus_tag="RHZ76_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HDV2811140.1"
                     /translation="MSTITLLCIALTGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGVVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(40806..41798)
                     /gene="rpoS"
                     /locus_tag="RHZ76_000439"
     CDS             complement(40806..41798)
                     /gene="rpoS"
                     /locus_tag="RHZ76_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HDV2811141.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(41861..43000)
                     /gene="nlpD"
                     /locus_tag="RHZ76_000440"
     CDS             complement(41861..43000)
                     /gene="nlpD"
                     /locus_tag="RHZ76_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HDV2811142.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPAVTTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(43140..43766)
                     /gene="pcm"
                     /locus_tag="RHZ76_000441"
     CDS             complement(43140..43766)
                     /gene="pcm"
                     /locus_tag="RHZ76_000441"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HDV2811143.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(43760..44521)
                     /gene="surE"
                     /locus_tag="RHZ76_000442"
     CDS             complement(43760..44521)
                     /gene="surE"
                     /locus_tag="RHZ76_000442"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HDV2811144.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(44502..45551)
                     /gene="truD"
                     /locus_tag="RHZ76_000443"
     CDS             complement(44502..45551)
                     /gene="truD"
                     /locus_tag="RHZ76_000443"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HDV2811145.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLNDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(45548..46027)
                     /gene="ispF"
                     /locus_tag="RHZ76_000444"
     CDS             complement(45548..46027)
                     /gene="ispF"
                     /locus_tag="RHZ76_000444"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDV2811146.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKMGFTGRGEGIACEAVALLIKATK"
     gene            complement(46027..46737)
                     /gene="ispD"
                     /locus_tag="RHZ76_000445"
     CDS             complement(46027..46737)
                     /gene="ispD"
                     /locus_tag="RHZ76_000445"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDV2811147.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(46756..47067)
                     /gene="ftsB"
                     /locus_tag="RHZ76_000446"
     CDS             complement(46756..47067)
                     /gene="ftsB"
                     /locus_tag="RHZ76_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HDV2811148.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(47261..47584)
                     /locus_tag="RHZ76_000447"
     CDS             complement(47261..47584)
                     /locus_tag="RHZ76_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HDV2811149.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(47634..48239)
                     /gene="cysC"
                     /locus_tag="RHZ76_000448"
     CDS             complement(47634..48239)
                     /gene="cysC"
                     /locus_tag="RHZ76_000448"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HDV2811150.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRYFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(48239..49666)
                     /gene="cysN"
                     /locus_tag="RHZ76_000449"
     CDS             complement(48239..49666)
                     /gene="cysN"
                     /locus_tag="RHZ76_000449"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HDV2811151.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEKTFTRICEDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMAWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(49668..50576)
                     /gene="cysD"
                     /locus_tag="RHZ76_000450"
     CDS             complement(49668..50576)
                     /gene="cysD"
                     /locus_tag="RHZ76_000450"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HDV2811152.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            50828..51865
                     /gene="iap"
                     /locus_tag="RHZ76_000451"
     CDS             50828..51865
                     /gene="iap"
                     /locus_tag="RHZ76_000451"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HDV2811153.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEVFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   51886..52768
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=52008..52036
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaactc"
BASE COUNT        12406 a        14376 c        13865 g        12153 t
ORIGIN      
        1 cacttttaaa tctttcaatc tgattagatt aggttgccgt ttggtaataa aacaataaat
       61 cctgaaggag agaacaatga tagaaaccat tactcatggt gctgagtggt ttatcgggct
      121 gttccaaaag ggcggagagg tgtttaccgg gatggtgacc ggcattcttc cgctgttgat
      181 tagcctgctg gttatcatga acgcactgat taattttatc ggtcagcatc gtattgaacg
      241 ttttgctcaa cgttgcgccg gtaaccctgt ttcccgttac ctgctgttac cgtgcattgg
      301 cacgtttgtc ttttgcaatc cgatgacctt aagcctgggt cgctttatgc cggaaaagta
      361 caaacccagc tactacgcgg cggcctctta tagctgccac tcaatgaatg gcctcttccc
      421 ccatatcaac cctggcgaac tgtttgttta tcttggcatt gccagcggtc tgacaacgct
      481 gaacctgcca cttggcccac tggcggtgag ttatctgctg gttggtctgg tcaccaactt
      541 cttccgcggc tgggtgaccg atctgaccac cgccattttt gagaaaaaga tgggcattca
      601 acttgaacaa aaagttcacc tggcaggagc aacatcatga cgcatattcg gatcgaaaaa
      661 ggaacgggtg gctggggcgg cccgcttgag ctgaaagcca cgccgggcaa aaaaatcgtc
      721 tatatcaccg ccggtacccg gcctgcgatt gttgacaaac tggcacagct tactggctgg
      781 caggctattg acggatttaa agaaggtgaa cccgcggagg cggaaattgg cattgcggta
      841 atcgactgtg gcggcacatt acgctgcggc atctatccga aacggcgtat tcccaccatt
      901 aatatccact cgacgggcaa gtccggtccg ctggcgcagt acattgtgga agatatttat
      961 gtctctggcg taaaagaaga aaacatcact gtagtgggtg atgcgacacc acacccctct
     1021 tccgtgggcc gtgactatga caccagcaag aaaatcaccg aacaaagcga tggtttactg
     1081 gcgaaggtgg gaatgagtat gggttctgcc gttgccgtgt tgtttcaatc tggtcgtgac
     1141 accatcgaca ctgtattaaa aaccattctg ccgtttatgg cattcgtttc ggcgctcatt
     1201 ggcatcatta tggcttctgg ccttggtgac tggattgccc acggtcttgc tccgctggcg
     1261 agccatccac tgggtctggt catgctggcg ctcatctgct ccttcccgct gctttcacct
     1321 ttcctcggcc caggcgcagt tatcgcacag gttatcggcg tattgattgg cgtgcagatt
     1381 ggtctcggca atattccgcc gcatctggct ttaccggcac tgtttgccat caacgcgcag
     1441 gcggcctgcg acttcatccc ggtcggtttg tcgctggcgg aagcccgtca ggacacggtt
     1501 cgcgtcggtg tcccttctgt actggtgagc cgctttttaa ccggcgcacc gactgtactg
     1561 atcgcctggt ttgtctccgg ttttatctat caatagaggc tgaaacatga ccgttattta
     1621 tcagaccacc atcacccgta ttggcgcgag tgccactgac gccctcagcg accagatgct
     1681 catcaccttt cgtgaaggcg cgccagcgga cctcgaagag tattgcttca ttcattgcca
     1741 cggcgagttg aaaggtgcac tccatcccgg tttgcaattt acgctcgggc agcatcgcta
     1801 tccggtgacc gctgttggca gcgtggcgga agacaacctt cgcgaactgg gtcatgtcac
     1861 cctgcgcttc gatggtttaa acgaagcgga atttccgggc actgtccatg tggcaggccc
     1921 tgtacccgac gatatcgcgc cgggatcggt tttgaagttt gaatctgtta aggagtaaaa
     1981 aatgaatcag gttgccgttg tcatcggtgg tgggcaaacc ttaggcgcgt tcctgtgcca
     2041 cggtctggct gccgaggggt atcgcgtcgc ggttgtcgat attcagagcg acaaagccgc
     2101 aaatgtggca caagaaatta acgccgaata tggtgaagga acagcgtatg gttttggtgc
     2161 tgacgccacc agcgagcaaa gcgttctggc gctctctcgt ggggtagatg aaatctttgg
     2221 tcgcgtggat ttgctggtct acagcgccgg aattgcaaaa gcagccttta tcagcgactt
     2281 ccagctcggc gattttgacc gttcgctaca ggtgaatctg gtgggttatt tcctgtgtgc
     2341 ccgtgaattt tcgcgtttga tgatccgcga cgggattcag ggtcgcatta ttcagatcaa
     2401 ctcgaaatcc ggcaaagtgg gcagcaaaca caactctggc tacagcgcag cgaaatttgg
     2461 tggcgtcggg ctgactcaat cactggcgct ggatctggca gagtacggca ttacggtgca
     2521 ttcactgatg ctgggtaacc tgctgaaatc gccgatgttt cagtcgctgt tgccacaata
     2581 cgcgaccaag ctgggtatca aaccggatca agtcgagcag tattacatcg ataaagtacc
     2641 gctcaaacgc ggctgcgatt atcaggatgt gctgaatatg ctgctgttct acgccagtcc
     2701 taaggcgtcg tactgcactg gtcagtcgat caatgtcacc ggcggtcagg tgatgttctg
     2761 atcaacagcg gagatccgtt aaggatctcc gtgagactaa tagaatgcct gatgcgctac
     2821 gctcatcagg cctacaggac ttccgccact acattaagga aaagttatgg tatccgcact
     2881 catcactgtc gccgttatcg cctggtgtgc gcaactggcc ttaggcggct ggcaaatttc
     2941 tcgttttaac cgtgccttcg acacactatg ccagcaaggt cgggttggcg tgggccgttc
     3001 cagcgggcgc tttaaaccgc gggtcgtggt cgccatcgcg ctggacgatc agcagcgcat
     3061 cgtcgacacc ttgtttatga aaggactgac catcttcgcc cgaccgcaaa aaattcccgc
     3121 aattaccggc atgcatgcgg gtgatttaca acccgatgtg atctttcccc atgatccact
     3181 atcacagaat gctctatcat tggcgcttaa actgaaacgt ggataatttc gttgtgaatg
     3241 ttacttgctt gcgaagttat cattttgaaa cctaaatcag gtaatcacgc ccatgaaacc
     3301 tcgtcagcgt caggccgcca ttctggagta tctgcaaaag cagggtaaat gctcggttga
     3361 agaattggcg caatactttg acaccacagg cacaaccatt cgcaaagatc tggtcattct
     3421 ggaacatgca ggaaccgtca ttcgtactta tggcggagtg gtgttgaata aagaggaatc
     3481 cgatccgcct atcgatcata aaacactcat caacacccac aagaaagagc tgattgcaga
     3541 agctgccgtt agttttattc atgatggcga ctcaataatt ctggatgctg gcagtaccgt
     3601 tttgcagatg gtgcccctgc tctcgcgctt taataacatc acggtgatga ccaacagcct
     3661 gcatatcgtc aatgcgctat ccgaactgga taacgaacaa actatcctga tgccaggcgg
     3721 aacatttcgt aaaaaatcgg cctcatttca cgggcagctg gcagagaatg ccttcgagca
     3781 tttcaccttc gataaattgt ttatgggcac cgacggcatc gatctcaatg cgggcgtaac
     3841 caccttcaac gaggtctata ccgtcagtaa ggcgatgtgt aatgccgcgc gcgaagtgat
     3901 tttgatggcg gactcatcaa agtttggccg taaaagcccc aacgtagttt gcagtcttga
     3961 aagcgtcgat aagctgatta ccgacgcagg tatcgatccg gcgtttcgtc aggcgctgga
     4021 agagaaaggg atcgatgtga tcataaccgg agagagcaat gagtgaagca ctactgaacg
     4081 cgggacgtca gacgttaatg ctggagttgc aggaagcaag ccgtttaccg gaacgtctgg
     4141 gcgatgattt tgttcgcgcc gccaatatca tcctgcactg cgaaggcaaa gtggtggttt
     4201 cgggaattgg caaatcgggc cacattggta agaaaatcgc cgcaacactt gccagtaccg
     4261 gcacaccggc tttttttgtc catccggcag aagcactgca cggcgatctg gggatgattg
     4321 aaagccgcga tgtgatgctg tttatctctt actccggtgg cgcgaaggaa ctggatctga
     4381 ttattccgcg tctggaagat aaatctatcg cgctgctggc gatgaccggc aaaccgacgt
     4441 caccgctggg cctggcggca aaagcggtgc tggatatctc cgtagaacgc gaagcctgcc
     4501 cgatgcacct tgcgccgacc tccagcaccg tcaataccct gatgatgggt gacgcgctgg
     4561 cgatggcggt catgcaggcg cgcggattta atgaagaaga ttttgcccgc tcccacccag
     4621 ccggggcact gggcgctcgc ttgctgaata aagtgcatca tctgatgcgc cgtgacgatg
     4681 ccatcccaca ggtggcgtta accgccagcg tgatggatgc gatgctggaa ctcagccgca
     4741 ccggtctggg gctggtggcg gtatgtgacg ctcaacaaca ggtacaaggc gtctttaccg
     4801 acggcgattt acgtcgctgg ctggtaggcg gcggggcact caccacgcca gttaatgaag
     4861 cgatgacgac aggcggcacc acgttacagg cgcaaagtcg cgccatcgac gccaaagaga
     4921 tcctgatgaa gcgcaaaatc actgccgcac cggtggtgga tgaaaacggc aaactcaccg
     4981 gcgcaattaa cctgcaggat ttctatcagg ccgggattat ttaatccttc attcccagac
     5041 gtttcgccag ccgatgcagg ttggcgacgt cggtttccag catccgcgcg caggcagccc
     5101 agttatgatg attttgtgcc agtgcctggc gaatagtttc acgctggaac gcttctgtcg
     5161 cttcacgcag gttttgctta acaacgggca ccgccgccgc ttctggcggc ggcaacgtca
     5221 cctcaggaaa agcaaaatgt tgcgcctcaa gaatcacttc atcgccgttg cgggtggctc
     5281 tcgccagcac taccgcccga tgaatagcat gttccagttc gcgcacgttc cccggaaaac
     5341 gatagtgttg cagtaaattt cgcgctccgg cacttaatac cacgcgggag agccccagcc
     5401 gcaaacgaca ctgctcgcag aaataccccg ccagcagaat gacatcatcg ccccgctcac
     5461 gcagcggcgg caccgaaagt ggaaacacgc tcaggcgatg aaacaagtca gcgcgaaatc
     5521 gccctgccag cacctcttcg cgtaagtcgc ggttagtcgc cgccagcacg cgcacatcga
     5581 cccgcaaact acggtcatcg ccaacgcgct gaatatcgcc atactgcaac accctcagca
     5641 gcttggcctg caatgccaac gacaactcgc cgatctcatc cagaaacaga gtgccgttat
     5701 ccgccatttc aaacttcccg ctgcggttac tgatagcgcc agtaaacgct cctttcacat
     5761 gcccgaacaa ctcactttcc gccacacttt ccggcagtgc agcacagttg agatagacca
     5821 gcggattcac cgcccgtggc gaggcttcat gaatcgcttt cgccaccagc tccttaccgg
     5881 ttcccgtctc accgctgatc aggacgttga gatcggacgc cgccacaatc tcaatctctt
     5941 ttttcagttg cgtcatgcca ggggacaagc caatcatctg cgtctgtttc accgcttcaa
     6001 acggcgtggc atcgcctggc atcatattct ggctttccag ttgttcaatc agcaacgcat
     6061 tgcttaacgc tcccgccgcc agcgcagcaa tcagccgtag ctcttcgtcg ctgaaaacat
     6121 cgaactgatc gggctgcatc ccgtcgagcg tcagtgcgcc gatcaggttt tgcccggcaa
     6181 acaatggcag accaacgcag gcgtgaacct tcagactctc ctgcccagga atcaaaccgt
     6241 catagggatc gggcaattcg ctgtctgcgg gaaagcgcac cacatccccg gcgcgggcaa
     6301 tcgcttccag ccgtggatgc ccttccagcg caaagcgtct accgagtaca tcctttgcca
     6361 gaccgtcgat ggcaagcgga ataaactgcc gcgaatcgta acgtagcaac gcagacgcat
     6421 cgcactccag cacctgacgt agcgtggtga tcaggcgctg aaaacgatcc tgatgaccaa
     6481 tcccacgctg caattcgatg gcgatattcg ccagcacatc aacggaaaaa ctcatctttg
     6541 cctcactgtc aatttgacta tagatattgt catatcgacc atttgattga tagtcatttt
     6601 gactactcat taatgagcat aattttattt atatagtaaa aacaatcaga taaaaaactg
     6661 gcacgtaatc tgcaattagc aagacatctt tttagaacac gctgaataaa ttgaggttgc
     6721 tatgtctatt gtggtgaaaa ataacattca ttgggttggt caacgtgact gggaagtgcg
     6781 tgattttcac ggtacggaat ataaaacgct gcgcggcagc agttacaaca gctacctcat
     6841 ccgcgaagaa aaaaacgtgc tgatcgacac cgtcgaccat aaattcagcc gcgaatttgt
     6901 gcagaacctg cgtaatgaaa tcgatctggc ggatatcgat tacatcgtga ttaaccatgc
     6961 agaagaggac cacgccgggg cgctgaccga actgatggca caaattcccg atacgccgat
     7021 ctactgtaca gccaacgcta tcgactcgat aaatggtcat caccatcatc cggagtggaa
     7081 ttttaatgtg gtgaaaactg gcgacacgct ggatatcggt aacggcaaac agctcatttt
     7141 tgtcgaaaca ccaatgctgc actggccgga cagcatgatg acttacctga caggcgacgc
     7201 ggtgctgttc agtaacgatg ctttcggtca acactactgc gacgagcatc tgttcaacga
     7261 tgaagtggat cagacggagc ttttcgagca gtgccagcgt tactacgcca atatcctgac
     7321 gccgttcagc cgcctggtaa caccgaaaat taccgagatc ctgggcttta acttaccagt
     7381 cgatatgata gccacttccc acggcgtggt atggcgcgat aacccgacgc aaattgtcga
     7441 gctgtacctg aaatgggcgg ctgattatca ggaagacaga atcaccattt tctacgacac
     7501 catgtcgaat aacacccgca tgatggctga cgctatcgcc caggggattg cggaaaccga
     7561 cccacgcgtg gcggtgaaaa ttttcaacgt cgcccgaagc gataaaaacg aaatcctgac
     7621 taatgtcttc cgctcaaaag gcgtgctggt cggcacttcg acgatgaata acgtgatgat
     7681 gccgaaaatc gccgggctgg tggaggagat gactggttta cgcttccgta acaaacgcgc
     7741 cagtgctttc ggctctcacg gctggagcgg cggtgcggtg gatcgtcttt ccacgcgcct
     7801 gcaggatgcg ggtttcgaaa tgtcgcttag cctgaaagcg aaatggcgac cagaccagga
     7861 cgctctgaag ttatgccgtg aacacggtcg cgaaatcgcc cgtcagtggg cgctcgcgcc
     7921 gctgccgcag agcacggtga atacggtagt taaagaagaa acctctgcca ccacgacggc
     7981 tgacctcggc ccacggatgc agtgcagcgt ctgccagtgg atttacgatc cggcaaaagg
     8041 cgagccaatg caggacgttg cgccaggaac gccgtggagt gaagtcccgg ataacttcct
     8101 ctgcccggaa tgctccctcg gcaaagacgt ctttgaagaa ctggcatcgg aggcaaaatg
     8161 agtaacggca ttgtgatcat cggttcgggc ttcgccgccc gccaactggt gaaaaatatt
     8221 cgcaaacagg acgccactat tccattaacc ctgattgccg ccgacagcat ggatgagtac
     8281 aacaaacctg acctcagcca tgttatcagt caggggcaac gtgccgatga ccttacccgc
     8341 cagacggcgg gtgaatttgc cgagcagttt aatctgcacc tgtttccaca aacctgggtg
     8401 acggatatcg atgccgaagc ccgtgtggtg aaaagccaga ataatcagtg gcaatacgac
     8461 aagctagtac tggcaaccgg tgccagtgcc tttgtcccgc ctgtgcctgg gcgtgagtta
     8521 atgctgacgt taaatagtca gcaagagtat cgcgcctgtg aaacgcaact gcgggatgcc
     8581 cgacgcgtgt tgattgttgg cggtggtttg attggtagcg aactggcgat ggatttttgt
     8641 cgtgcaggca aagcggtcac gctaatcgac aacgctgcca gtattctggc gtcgttaatg
     8701 ccaccggaag taagcagccg cttgcagcat cggttgacgg agatgggcgt tcatctgctg
     8761 ttgaaatctc agttacaggg gctggaaaaa acggattctg gcattcaggc aacgctggac
     8821 cgccagcgca atatcgaagt ggatgcggta attgccgcca ccggactgcg cccggaaacc
     8881 gccctggcac gacgcgccgg gctgacgatt aatcgcggcg tttgcgtcga tagttatctg
     8941 caaaccagta ataccgatat ttacgcgctg ggcgattgcg cggaaattaa cggtcaggta
     9001 ttgccgttcc tccagccgat tcaacttagc gcgatggtgc tggcaaaaaa tcttctcggc
     9061 aataacacgc cgctgaaact cccggcgatg ctggtgaaaa tcaaaacgcc ggaattaccg
     9121 ctgcatctgg caggcgaaac ccagcgtcag gatttacgct ggcaaattaa taccgaacgc
     9181 cagggaatgg tggcgcgcgg cgttgacgat gctgaccagc ttcgcgcctt tgtggtcagt
     9241 gaggatcgga tgaaagaggc atttggattg ttgaaaacat tgccgatgta ggtgggctac
     9301 tgtgcctaaa atgtcggatg cgacgctggc gcgtcttatc cgacctacgg ggacgcatgt
     9361 gtaggccgga taaggcgttt acgccgcatc cggcaatggt gtccaaatgc aacacgtttt
     9421 atccgttctg gacttcaccc gctaaccaac gcgccgcagc aataaccccc tgccccagag
     9481 acaaaccgcc atcacccgcc ggtaaactct gtggaaagag caatgtgaaa tcagcgagat
     9541 aatgcgccag acgtgcacgc agcaaacggt tatgaataac cccgccgcta aataccagcg
     9601 tagtgatacc acgcatcgtg gcctgctcac gcatcaacgc ggcaaaaccc tgcgccagcg
     9661 catcatgaaa cgcccacgcg cgttgattaa ccggtgcctg ccagttcagc cactgctgcc
     9721 agaaagtggc gagatccagt tgattgtcca cccgcggcat tgtcaccgga tgcgtcactc
     9781 cgtggcatga ggctgcgagc gcctccagag cacaagccgc ttcaccttca taacttaacg
     9841 tggctggcgc acagcccagt gccgccgcca ctgcatcgaa aaaacgccca cacgatgacg
     9901 ccagcggcgc gttaattcca cgctcaatgg cccgcgccag cacgctccag ttttgctgtt
     9961 gcacactcgc tgtttcggga taattctgcc actccggcac aaagcgcagg cactgcgcca
    10021 gcaggtttcg ccacggctgc ttcgccgcca aatcgccacc agcaagcgcc actgcaggca
    10081 agccgcccag gtgctcacat tcgcgatagt tcacccgcag gcactcgccg ccccacaaag
    10141 cgccattctc ccccataccg ataccatcga gcgtcaaagc aatgacatca ccgccatcca
    10201 gcggccactg atgctctgcc agacacgccg ctgcgtgggc atgatgatgc agcaccgttt
    10261 gcgtcggcag attcatttcg cgcgcccact ggctggagac atagcccgga tgtgcgtcat
    10321 gcacaacgta ttgcggggtg aaatcgtaga tgttttgcat caggcgtaac gcttcgcgcc
    10381 actgcatctg gatgccatcg tcacttaaat cgcccagatg ctgactcaac accgcttgtt
    10441 caccgcgcac caggcagaag gtatttttca gatccgcgcc gagacacagc acaggcggaa
    10501 catttttaaa gcccggaggc aaagccagcg catccggcac atacccccgc gaacggcgca
    10561 gcatttcgcc gctttcgcgc accaccgaat catccatccg ctgcacgatg tcgcggttat
    10621 gtatcaaaaa tccgtcggca atgccctgca aatccgccag cgcctgttcg ttgctgatag
    10681 ctggtggttt accgctcagg ttgccggagg tcatcaccag cgggcattgc agttcctgta
    10741 acagcaaatg ctggagcggg ttcgcaggca acattacccc gacttcgtta aggtcagggg
    10801 cgatatcatc acaaagctca ggaacgtatt ttttatccac cagcacaatc ggcgcggcgg
    10861 gcgtggtaag caactggcgc gcagcgtctg gtaaaccgtc agccactggc aacatgaccg
    10921 ccagcggttt cgccgggcga tgtttgcgcg cccgaagtgt cgccaccgcg ttactgttac
    10981 gtgcatcgca ggcaagatga aatccgccaa tccctttgat ggcgacaatt ttgcccattt
    11041 ttaactgtgc gatagctgcc tgtaatgccg cctcttgttc cgcatgttca ccatgactta
    11101 cccattcaag atgcgggcca cactccgggc aggccaccgg ctgggcgtgg aagcgacgat
    11161 cgagcgggtc acggtactct ttgtcacagg ccggacatag cggaaacgcc gccatcacgg
    11221 taaacgggcg gtcgtaaggc atggcgcgaa taatggtgaa acgcgggccg cagtgggtac
    11281 agttgataaa cggataacga taacgccgtt cgcctggggt attcatttcg gcaaggcaag
    11341 cagggcaagt agcggcatcg ggaacaattt gcgtattcat ggtgccgcct gtgctctggc
    11401 gtatagtgaa ctcggtgggc agttgtgacc agataaacgg ctcacgctcg acgctatcaa
    11461 tacgcgccag cggcgggcag tgctgataca attgaacaag aaacgtttcc gggtcttccc
    11521 gcagccggac ttctacgcca tcgccgtcat tacagacatc gccgtgaaga tttaattgct
    11581 gtgccagctg ccagacaaac ggacgaaaac cgacgccctg cactttgcca cgaatacgca
    11641 gttggacacc gcaagatgtg ttttttgcca ttgagttatt cccgccatca tgaattgcgt
    11701 aacccgccct gccggacacg acagcgtcgc atccggcagt cacaggtcgg cgataccgcc
    11761 cgctccgtat tctacgaata tttccgggaa ttcctttgat gccagaacag ttctgtaaga
    11821 tttttagaac atcagcgccg tacggcggcg tttttctgcg ctcagttgtt caagtttatt
    11881 acgatcgaca caaatcagcg catgagtcgg gcaagccgcc atacacgccg ggccgtcttc
    11941 acgatggttg cacaggtcgc atttattggc ttcggctttg tcagcccgta cattcagacc
    12001 cgcgccgctg ttgcggatca ccggacgtac caccacttcc atcgcaccat acgggcaagc
    12061 cacaacgcag gttttgcaac caatgcaacg ttcctgcatc acatgaacaa accctttatc
    12121 acggctgata gcaccattcg ggcagacgtt agcgcacggt gcatcttcac actgacggca
    12181 aactgtcgcc gtggaaatgt tcacaccttt aatgacatgg atacgcggta aaaaagtttc
    12241 cggggtcagc gatgcacagt cctgattttc ctgatgagaa accacgcacg ctacttcaca
    12301 ggtacggcaa ccaatacatt tactcgcgtc agcaatgatg aaacggttca tcaaattctc
    12361 cagcaatgac agttaatgcg ccgatacatt cacaaatcat gccagttttt aatttactgt
    12421 tatttaagga aattaatttc tgtaatgcag gaaaaacgat gtcatcgaca ctagtgacga
    12481 tgacatgtga tgacaatgtt tatcgcgaag gagcaatgag tgagtcgcgg cggatcagtt
    12541 ttccgctgaa ggttttcggc ggtgagaaat ccccgccatc gagcataaaa atcagccgtc
    12601 caataatttc ctgaatcatc tcagtcaccg gaatttttac gctggagagc gccggaacgg
    12661 tgtagggggc aatagcgata tcatcgaatc cgataactga cacctgctct ggcaccgcta
    12721 cgccgcgctc gtgtaacgct ttcatcgcac ctatcgccat atcgtcgtta ctggcaacta
    12781 acgcgctaaa tttagcccca cgttcgagca acatttctac cccttcggcc ccgctggcag
    12841 gcgtccattt accgttagcg ataagttttt cattgagcgc aataccatgc tgcgccagcg
    12901 cgtctttata cccggcaaga cgttcaatgc tggtggggga atccatcgag ccggtaagga
    12961 aagcaatctc ctgatgcccg gcgtttatca actctgccac ggcgttaaaa ctggtctgtt
    13021 tatgatcgca ccagacgcta tggctgctgt ttttgcgcag gcggcgatta agcaccatta
    13081 tcggctgact gtgcgcgtca atgatgtcat cgatctcatc cacgcttaaa aaacgcgggt
    13141 aaatcatgat cgcgtcgcag cgcagatcca gcagatactg aatcgcctgg cgctcttctt
    13201 ctgcgctgtg tttaccatct gccaatagca actgccgccc tttctcttcc gccattcgcg
    13261 cggcatgaaa gagtaattca ctaaaataaa tgccgtggta aagcgtgttg gtcactacca
    13321 gccccagcgt ctgagtactc ttcgccgaca gattgcgcgc cagcaagttt ggacggtaac
    13381 cgctctcttc taccgcctga aacacgcgat ctttagtctc ctggctgacg tagccattac
    13441 ctgaaagcac gcgggaaacg gtcgcttttg aaaccccggc gcgcttcgcc acttccagca
    13501 tcgtcgtcat cattttcatc cctttacacg caatcaacgc agtgtactgc accgtttgcc
    13561 gattgtcctt gcacaatcgg cgggaaaaat attcaggtga ccggtttcac aaatataaaa
    13621 aatgaacaat tcactctctt gcttatttag tgacaactat tcatgatttt gtgaaaccgg
    13681 tttcttaatt ccgtttcagc atcggcattt ttccgtcacg tcgactgata acaactacat
    13741 ctaccctact gataacagga taaaatccga tggccaaaaa ttatgcggcg ctggcacgct
    13801 cggtgatagc ggcactgggc ggcgttgata acatctcggc ggtcacgcac tgtatgacgc
    13861 ggttgcgctt tgttatcaaa gatgatgcgc ttatcgacag cccgacgtta aaaaccatcc
    13921 ccggcgtgct cggcgtggta cgtagtgaca accagtgtca ggtgattatc ggcaataccg
    13981 tttcacaagc ctttcaggaa gtcgtcagcc tgctgccggg agatatgcag cccgcacagc
    14041 ccgtgggtaa acccaaactc acgctacgtc gcattggtgc ggggatcctc gatgcgctga
    14101 tcggcaccat gtcaccgctg atcccggcga ttatcggcgg atcgatggtc aaactgctgg
    14161 caatgatcct cgagatgagc ggcgtgctga caaaaggatc gccgacctta accattctga
    14221 atgtgattgg tgacggtgct ttcttcttcc tgccgctgat ggtcgcggca tctgccgcca
    14281 tcaaatttaa aaccaatatg tcgctggcga ttgccatcgc gggtgtgctg gtacatccga
    14341 gttttattga actgatggcg aaagcggccc agggtgaaca tgtcgaattt gccctgattc
    14401 cggtcaccgc ggtgaaatac acctacacgg tgatcccggc gctggtgatg acctggtgcc
    14461 tgtcatatat cgaacgctgg gtggacagca ttacaccggc ggtgaccaaa aacttcctca
    14521 aaccaatgct gattgtgttg attgccgcac cgctggcaat cctgctgatt ggcccgattg
    14581 gtatctggat cggtagcgcc atttcggcgc tggtttacac cattcatggt tatctgggct
    14641 ggctttcagt cgccattatg ggcgcgctgt ggcctctgct ggtaatgacc gggatgcacc
    14701 gcgtctttac gccaaccatc attcagacca ttgccgaaac cggcaaagaa gggatggtca
    14761 tgccgtcaga gatcggcgct aacctgtcgc tgggcggttc atcactggca gtggcgtgga
    14821 aaacgaaaaa cccggaactg cgtcagacgg cgctggctgc ggcggcatca gccattatgg
    14881 cggggatttc cgaaccggcg ttatatggcg tggcgatccg cctgaaacgt ccgcttatcg
    14941 ccagtcttat cagcggtttt atttgcggcg cggttgccgg tatggcgggg cttgccagcc
    15001 actcaatggc agcgccgggg ctatttacca gcgtgcagtt cttcgatccg gcgaatccaa
    15061 tgagcatcgt ctgggtgttc gcggtcatgg cgctggcggt ggtgctgtcg tttatcctca
    15121 cactgttgct cggctttgag gatattcctg ttgaggaagc ggctgcccag gcgcgaaagt
    15181 atcagagcgt acaaccgacc gtcgccaaag aagtaagtct taattgagga tgaaaatgtc
    15241 agtatttcca gaaagttttt tatggggcgg cgcgcttgcc gccaaccagt ctgaaggtgc
    15301 gttccgtgaa ggtgacaaag gtctgaccac tgtcgatatg atcccacacg gcgagcatcg
    15361 aatggcggtg aaactggggc tggaaaaacg ttttcagttg cgagatgacg agttttatcc
    15421 cagccatgag gcgacggatt tttatcatcg ttataaagaa gatatcgccc tgatggcaga
    15481 gatgggattc aaggttttcc gtacctcaat tgcctggagc cgtctctttc cgcagggcga
    15541 tgaaatcacg cccaatcagc agggcattgc tttttatcgt tctgtctttg aagagtgtaa
    15601 aaagtacggt atcgaaccgc tggtcacgtt gtgccacttc gatgtgccga tgcatctggt
    15661 caccgaatat ggctcctggc gtaaccgcaa gctggtggag tttttcagcc gctacgccag
    15721 aacctgcttt gaagcatttg atggtctggt gaaatactgg ctaaccttca atgaaatcaa
    15781 cattatgttg catagcccgt tctccggcgc gggtctggtg tttgaagaag gtgaaaatca
    15841 ggatcaggtg aaatatcagg ccgcgcatca ccagctggtt gccagtgcgc tagccaccaa
    15901 aatcgcccat gaggttaacc cgcaaaatca ggtgggctgt atgctggcgg gcggtaactt
    15961 ctacccttac agttgcaagc cggaagatgt ctgggcggcg ctggagaaag accgggaaaa
    16021 cctgtttttt atcgatgtgc aggcgcgggg cacgtatccg gcttactctg cccgcgtatt
    16081 ccgcgaaaaa ggggtaacca tcaacaaagc accgggcgat gatgaaatcc tgaaaaacac
    16141 cgtcgatttt gtctctttca gctattacgc ctcgcgctgc gcctcggcgg agatgaacgc
    16201 caacaacagc agtgcggcga acgtggtgaa atcgctgcgt aatccgtatc tacaggtgag
    16261 cgactggggc tggggaattg atccactcgg tctgcgtatc accatgaata tgatgtacga
    16321 tcgttatcag aagccgctgt ttctggtgga aaacggcctg ggcgcaaaag atgaatttgc
    16381 tgccaatggc gagattaacg acgactatcg catcagctac ttacgcgaac atatccgcgc
    16441 aatgggcgaa gcgattgcag acggcattcc gctgatgggc tacaccacat ggggctgtat
    16501 tgatttagtt tccgcctcta cgggtgaaat gagcaaacgc tacggctttg tctttgttga
    16561 ccgtgacgac gcaggcaacg gtacgctgac gcgcacgcgt aagaaatcat tctggtggta
    16621 taaaaaagtg attgccagta atggggaaga tttagagtag gtaacagtgc cggatgcggc
    16681 gtgaacgcct tatccggcct acatgtcgca cgaaccatga aggcccgata agcgcagcgc
    16741 atcgggcaat ttagcatttg gcatcagagc tggttcaccc catcaagaac atccctgtcc
    16801 tgattcctta atgaaaaact actcttcttc caccgctaac tgcgcgaagc cgccatttcc
    16861 ttcccagcct tccagtcgtt gataaacggt ttctaccgca tctttaatcg gctgggtcat
    16921 cgggtagtaa aagccgacga tatccggctg aatgccgagg aaaatcactt cgccaatatc
    16981 ttctttcaac tggtcgataa ggtaattcaa cggcatgtta tgggtagtca tcataaacat
    17041 ctcggcgata tcatccgggt cgatgatgcg gatctcgccg gggtttagcc ccatatccgt
    17101 ggcgtcgaca atcagcagtc gtgtcgggcg cagttcacgg atagcgacga tgtcgttttc
    17161 cggtgcgcta ccgccgtcaa tcaccaccca gttacctttc ggcgcggcgg cgcacttttc
    17221 cgccagcagc ggacctgcgc catcatcgcc catcatgcta ttgccaacac agagtaaaac
    17281 gtcagtcacg cagtctcctc accatcaaat agatggcgtt ttcctgatga atatcatgca
    17341 gcatacttaa cagcattgta ctccattcct gctgctgcga agtttgcgct gcccgggctt
    17401 tatcgaatgc gttggccagc atcggcacat ggttaatgtc gatgacgatc tcaccatatt
    17461 tcggcacgcc ttccattttg cggcgggctt cactgcctgc ctccagagtg gcaatccatg
    17521 ccagatattc atcccacggg caggtgagcg cagcttccag gcaatcgata accccaaggt
    17581 ggtgaccaat cgccaggctg taatagacca cctgctgcgc ctcggcgggc gtggcatcgt
    17641 tctcatcaat aaatttacgg ctcagttgac tgaacaccac cttttcactc atcggatacg
    17701 cgcctcttca acaacatgat tcagatggct gacaatctcg ttcagacgcg gatcgttttc
    17761 cgcttccagc cagcgtgcaa cctgttcttc gccctgccct aactgtgtaa ggtaatcatc
    17821 ggcaatctga cgaccgtaac gataacccgc caggcgacgt gcttcgcgat ccactttcac
    17881 gcgcagcggc tgcaccatat cgccatgcag gatctccgcc ggttgttcat ccagttcacc
    17941 cggcccacgg gcgtgaattt tctgctccag caggccgagc gccattgcaa agccgtacag
    18001 cgtggcggca ggcgttggcg ggcagccagg gatataaaca tccacaggga caattttatc
    18061 cgtaccgccc cacacgcagt agagatcgtg gaagatcccg ccgctgttac cgcaggcacc
    18121 gtaggagata caaattttcg ggtccggcgc ggactgccac gcacgcagcg caggggatcg
    18181 cattgcacgg gtgaccgcgc cggtaaacag taaaatatcc gcatgacgcg gtgaaggaac
    18241 gactttaatg ccgaagcgtt ctgcatcaaa cagcggcgat aaagtggcga aaatttcgat
    18301 ttcgcaaccg ttgcagccgc cgcagtccac gcggtaaaca taggcagaac gtttgatttt
    18361 tttcagtaac gacgccttca tgctggcgat ggattcatcc accgtcatgg ggaccggaat
    18421 gccgttggcg tcacgggggc ctaataaatt gctcatcaga tggcctcttt catatggcga
    18481 gtcagttcaa tacggtcgga cggcaccagg catttctggc gcttacattc cgggcaagtc
    18541 tcaaagcttt cgcggtggtt ttccgcgcgg ctgtcgccgt tgtgcttaag cagcgcaatg
    18601 gcgtagtcga tctctttctg gacggcgaaa ggacgattgc agacgcggca gttgcacagc
    18661 gcgaagcggg actgttgcag gaagtcttct ttcttccaca ccgccagttc gtactcttgc
    18721 gacagtttga tcgccgccgt cgggcagact tcttcgcagc gtccacagaa gatgcagcgc
    18781 ccaagattaa actcccaggc aagctctcct gtggcgaggt cagtttcaac cgttaaggcg
    18841 tttgacgggc aggcattgac gcaggccgcg cagccgatgc actgctgcgg gttctgctct
    18901 ggcttaccac ggaagttttt atcaaccgca atcggctcca gcggataaga cgaggtcgcc
    18961 gtgccggttt tgatgacttt tttgataaag gtaaacatgg cgattcctta tttcagcggc
    19021 gagtttttac gctcaatgct gtaacgctcg agttctttgt acggcaccac tttgctcttc
    19081 ttcttacgca catcgaccac ggtcatgcgg tcggtacagg agtagcaagg gtcgaggcta
    19141 ccgataatca gcggcgcatc ggaaacggtg ttgccgcgca gcatgtagcg cagggtcggc
    19201 cagttcgcgt aggtcgcggc acggcagcgc cagcggtaca gcttctggtt gtcgccggtc
    19261 atgctccagt ggatatcatc gccgcgcggc gcttcggcaa agcccagcgc aaagcggtgc
    19321 ggaatgtagg taaagccttc caccatcagt gggccacccg gcaggttatc cagaccgtag
    19381 tcgatcatgt tcagcgcggt atagacttcg ttgatacgca ctttcagacg ggaaataacg
    19441 tcgcagccct gctcgctgtg gacttccatt ggcagcaggc catagccgac aaacgggtga
    19501 tcggcgcggg tatcacgggc gtgaccgctg gcacggacca tcgggccgac gttactgaag
    19561 tcgcgagcga tttccgggtc cagacgacca atgccgacag tgcgctgttc catgttcgga
    19621 gtgctcagca gcacatccac cagctcctgc acttcacgac gcatctgttg tgccagctgg
    19681 cgggtctgga tcatgtcgtc tttcagcaga tcgcgacgaa tcccgccgat caagttcagg
    19741 ccgtaggttt tacgcgcacc ggtaaggatc tctgccattt tcatggaggt ttcacgcacg
    19801 cggaagaact gcataaagcc ggagtcgaag ccggtaaagt gacaggccag gccaaggttg
    19861 agcagatgcg agtgcaagcg ttctacctcc agcagaatgg cgcggatcat ctgcgcacgt
    19921 tctggcacct gaatacccat cgcgttttcc accgacgtgg tgtaggcggt gctgtgggca
    19981 aagccgcaga tcccgcacac acggtcagag aggaaggtca cttcgttata acccatacgg
    20041 gtttccgcca gtttttccat gccgcgatgg acgtagaaca gacggtagtc ggcgtcgata
    20101 atgttttcgc catcgacgaa cagacggaag tggcccggtt catcagaagt gacgtgcagc
    20161 ggaccaatcg gcacgacgtt gtttttcttg tcgcccagtt cgttgatgaa ctcgtaggtt
    20221 tcagcatcgg tggtcggtgc cggacgctga cgataatcca tgctgtcttt acgcagcgga
    20281 taaagttcat ccggccagtc atccggcagc accagacgac gttcatccgg cagaccaacc
    20341 ggaatcaaac cgtacatatc gcgcacttca cgctcgcccc acaccgccgc cggaacgcgc
    20401 ggcgtcacgg acggatattc cggtttgttg gcgtcaactt cgacgcgaac cgtaatccaa
    20461 cacttagtgc ccttctccat cgacagcacg tagtaaacgg cgtaatgacc attcagtttg
    20521 cgttcgtcgt taccaaacag caccgacagc cagccaccct gtttgtagta aagaaactcc
    20581 accacttccg gcaggtagtt cacctttacg gtgacagtca gctgatcttt ggtctgccag
    20641 gcgtggtcca gcacgacgcc cggaaatgcc tcattcagcg cggcgagata atgttgacct
    20701 aatttttctt cagacatgct caaactctct ttaatcacgc cgccagcaag gagacgaacg
    20761 ctaaaaatgc aaagccaaac ccagcccagg taatgcgcgg agtaatatca agacgcagac
    20821 gggccatgct gttttcgaac agcgcgataa ccaggacgcc gaccaccagt tttacgatgg
    20881 caatcaccag cgccagcagc agtccaccgg cggtgaaggt ttccatttgt ccccacggaa
    20941 taaacacccc gacgaacatc tgcaacacca ccagctgttt caggctgata ccccatttca
    21001 tgacgccaaa gccgctgccg ctgtattcag agagcgggcc ttcctgcagc tcctgctcgg
    21061 cttccgccag gtcgaacggc agtttgccca tttcgataaa ggtggcgaac gcacaggcac
    21121 aaagcgccag taccagcggg atgctctggc tcagcggcca gtgataaacg gtgtcggtga
    21181 tgttgctgat gttggtggaa ccggcaacct gtgcggcaac ccacagacca agcagcagca
    21241 tcggttcgac cagcacgcca agcatcgctt cacggctcgc gccgatagcg gtaaacgggc
    21301 taccggtatc cagaccagaa atggcaaaga agaaacgcgc gatggcaaag agatacagta
    21361 aggtgatcaa atcacccagt tgcggcagcg gagaaccgac ggtcaccacc ggcagcgcag
    21421 tagcgatagt cagcatgacg cccaccatca catacggcgt caggcggaac acccagccgg
    21481 aggcatccgg gccgacgctc tgacgcccca gcagtttgat aatgtcgcga tactcctgca
    21541 acacgcccgg cccgcgacgg ttatgcaagc gggcgcgcgc cacgcgggtt ataccggaga
    21601 gcagcggcgc aacggcaaat aacaccagcg cctgaattaa cggatataaa acactcattc
    21661 tcaggctcct cgtgaaacaa taatcaccac cagtaccgcc agttcaacca gcgccatccg
    21721 gcggaacagc aacgcactcc cctcgcactg ccagcccggc accagagaca caggattcag
    21781 ccatttgcgt agtttcagca ccgccgcaaa cgcctgtttc accggcatgg caaaaccgtg
    21841 agcggtaatc accattgatt tttcgtggtc gtaaccgcac acccaggccg caccgcggga
    21901 acgcgatggc aaacgatcgc ctttgcaaat cgccataatg atgaatggca gcagcgggca
    21961 ggcaatcagc agcaacgtga tcatcggttg agaaacggtg gtgttagcag gctccagcgg
    22021 cagaggtaca gcagcagaga gcatcggcag tagccacggc gcagcaacac cgccaattac
    22081 gcagcaaatc gccagtgcca ctacgcttac gctcatcagg agcggcgcac aggtggcgtt
    22141 ttcggcttct ttggtacgcg gcgcgccgag gaacgtgacg ccatagactt tcgccataca
    22201 catcaccgcc agcgcaccgg taattgccag ccccacagcg agcagcggcc ccagcagacg
    22261 ggcaacaaac gcgccactat tgctcagttt gaaaaatgat tgatagataa cccattcccc
    22321 ggcaaaacca ttcagcggcg gcagcgcagc cattgccatc agcccgacta acatggcgat
    22381 ggagataacc ggcattttct tgccaatacc accgagtttt tcgatatcgc gatgaccggt
    22441 acggaaccag acgctccccg ccccgaggaa cagtacgctt ttgaacaggc tatggttaag
    22501 cagatggtac agaccaccga ccaggccaag agcaatcagc gccggttgtt cgagcgcgat
    22561 acccgttacg ccagcgccca gccccagcag gatgatgccg atattttcca gggtgtggta
    22621 agccagcagg cgctggatgt tgtgctccat cagcgcatac agaccaccga caaacgcggt
    22681 gatcatgccg agcaccagca gcgcgatccc ccaccacagc ggtgcattac cgcccagcag
    22741 tgacagggtt aaaatgccca gcaggccaat tttcatgact accgtagaaa acaacgcggc
    22801 agctggcgca gaggcgttcg catgtgcctg cggcacccag ccgtgcagcg gaataatccc
    22861 ggccagcagg ccaaagccaa tcactccgag cagccagata tcggaaccga gcggcagctg
    22921 ttgcatacgc atatccagca ggcgcagatc cagcgtgccg taacgctgcc acagcagcca
    22981 gcaagcaatc gccagcagca gagtgccaag acgccccagc gcaaaccaca gtttgccctc
    23041 tttgctgttg ctggtgagga acaccgcgca cagggccatg atttcggcca ttaccacgaa
    23101 catgccgagg ttgctggcaa tgacggcgca gacggcggca gccatcaaca tattgatctg
    23161 caagccgttg cacttcacct gcgcgtggcg atgccagtca atgttgtaga ggctgacaaa
    23221 cagaccgcac agaccgagcg taatcagcca aatcgcgtta agcggagaga tttgcacatc
    23281 gtagcttacc agcgacagcg caccgctcac gccaaccgcg ccagtcagta cagtgaagcc
    23341 cgcggctgcc gtatacagac taccaaccgc gccgccaatt ccagctatcc agccacttaa
    23401 cgctttttga aaagaaaaga gaaatgccag aacagcggcg gcgacaaacc acgccacgcc
    23461 gctattgatc agggaaattg cgctcattta gcctctccac tttgagcctg ctgaaacaag
    23521 gtgagatcgc caaagtccgt gttaaaggtc agctcacgct tacgtttgct gacgcgggcg
    23581 atatcggtgt tatccaccag atgcagggct ttagtcgggc acatccgcac gcaggccgga
    23641 ccttgttcat caaagctaca aaggtcacat ttgacggcga tcgcgcgaat acctggcacc
    23701 cagtcaagca atgtgctgac acgcgccgga gcaggcggtg ccggtggcgc tttcggggta
    23761 ttggcgtttg ccggaatatc cagcggacgg ctgccggaaa attcaattgc gccaaacggg
    23821 caggcgatgc cgcacagctt gcagcttacg cacaggcttt cattcaactg cacggcccca
    23881 tcaacgcggg tgatggcgtt aaccgggcag accaccgcgc agggtgcatc ttcacagtgg
    23941 tgacagagct gcggcgcaga ttctttttca ttcagcatca ctctcaggcg cggcattgat
    24001 tgcaggccgt gctggcgatg cgtctctgaa caggcggcct cacaagtgtg gcagccgata
    24061 cagagcgtgg agtcagcaat tacaaaacga ttcaccaggc attcctcagg tgattgtcat
    24121 ttttgacgaa aacatgccga tgaaatgtca ttttcgacac tcatcgacac gcccatcccc
    24181 aaacaggcgt aacgcctgca aaacgggcaa agcctcagct catgctgccg ggctttgtcc
    24241 ctttaccagt tggcttaaat tcaccggcac attgttttca atagcggtgt ataagctgtc
    24301 gtaaacgcca gcgatttttt cgagatagtg ttccagaacc tgttcgcgat cgcggatgtt
    24361 gaccttgcca tcaatcatgc cgtcaatgct cagttgcgcc tgcgcaatcc gctgtttgtc
    24421 ttcgccatct tttgtttcga cgtaatactc gatggtgtgg ctgttaaagc catccgccag
    24481 ggtgacgtaa atgcgaaaat gttcaaaaag gacgaaacag agttctttgt ccggcgcgca
    24541 gctcatggcg tgatgcaggc gcgctttcga taacgtgatc ccctgaacca gcgaattgca
    24601 gtagatgtgc cactggtcct gtaggcgacg atgccgctgt gcgatgtaat cggctttctc
    24661 gcttatttcc caaatagtca ttgtcaggtt acccgtttaa cagagatgcc agctttaagc
    24721 attttttgtg ccaactttta atttattgtt attaaagaga tttttaagct aaagatgaat
    24781 ttcgtcgccg tgtcgacgtg tcatttcgac atcatcgaca ttattcaccg cagggataat
    24841 caacactggc acaattattg cttgtagctg gcaatagtta atgggaggcg atatgcacga
    24901 aataaccctc tgccaacggg cactggaatt gatcgaacag caggccgcaa aacacggcgc
    24961 aaaacgcgta actggggtct ggctcaaaat tggcgcattt tcttgtgtcg aaaccagctc
    25021 tcttgccttt tgttttgatc tggtttgccg cggcagcgtg gcggaaggtt gtaaactgca
    25081 cctcgaagaa caagaggccg aatgctggtg tgaaacatgc caacagtatg tgacgctact
    25141 gacccagcgc gtccgccgct gtccacagtg tcatggtgac atgctgcaga ttgtggcaga
    25201 cgacggttta cagattcggc ggatagaaat agaccaggag tgagcgatgt gtacaacatg
    25261 cggttgcggt gaaggcaacc tgtatatcga gggtgatgaa cataaccctc attccgcgtt
    25321 tcgtagcgcg ccatttgccc cggcggcacg cccgaagatg aaaatcaccg gcattaaagc
    25381 gcctgaattt acccccagcc agactgaaga aggcgacctg cattacggtc atggcgaagc
    25441 gggcactcac gcaccgggca tgagccagcg tcggatgctg gaagtcgaaa ttgacgtgct
    25501 ggacaaaaat aaccgtctgg ctgaacgcaa ccgcgcgcgc tttgctgccc gcaagcaact
    25561 ggtgctcaac ctggtttcca gccctggttc cggtaaaacc accctgctga cggaaaccct
    25621 aatgcgcctg aaagacagcg ttccgtgcgc agttattgaa ggcgaccagc aaaccgtgaa
    25681 cgatgccgca cgcattcgcg ctaccggcac accagcgatt caggtgaaca ccggtaaagg
    25741 ctgccatctt gacgcacaga tgattgccga cgccgcaccg cgtctgccac tggacgataa
    25801 cggtattctg tttatcgaaa acgttggcaa cctcgtatgc ccggccagct tcgatctcgg
    25861 tgaaaaacac aaagtggcgg tgctttccgt taccgaaggt gaagacaaac cgctgaaata
    25921 tccgcatatg tttgccgccg cctcgctgat gctgctcaac aaagttgacc tgttgccgta
    25981 tctcaacttt gacgttgaaa agtgcatcgc ctgcgcccgc gaagtcaatc cagaaattga
    26041 aatcatcctt atttccgcca ccagcggcga agggatggac cagtggctga actggctgga
    26101 gacacagcga tgtgcatagg cgttcccggc cagatccgca ccattgacgg caaccaggcg
    26161 aaagtcgacg tctgcggcat tcagcgcgat gtcgatttaa cgttagtcgg cagctgcgat
    26221 gaaaacggtc agccgcgcgt gggccagtgg gtactggtac acgttggctt tgccatgagc
    26281 gtaattaatg aagccgaagc acgcgacact ctcgacgcct tacaaaacat gtttgacgtt
    26341 gagccggatg tcggcgtgct gttgtatggc gaggaaaaat aatgcgtttt gttgatgaat
    26401 atcgcgcgcc ggaacaggtg atgcagttaa ttgagcatct gcgcgaacgt gcttcacatc
    26461 tctcttacac cgccgaacgc cctctgcgga ttatggaagt gtgtggcggt catacccacg
    26521 ctatctttaa attcggcctc gaccagttac tgccggaaaa cgttgagttt atccacggtc
    26581 cggggtgccc ggtgtgcgta ctgccgatgg gtagaatcga cacctgcgtg gagattgcca
    26641 gccatccgga agtcatcttc tgtacctttg gcgacgccat gcgcgtgccg gggaaacagg
    26701 gatcgctgtt gcaggcaaaa gcacgcggtg ccgatgtgcg catcgtttac tcgccgatgg
    26761 atgcgttgaa actggcgcag gagaatccaa cccgcaaagt ggtgttcttc ggcttaggtt
    26821 ttgaaaccac tatgccgacc accgctatca ctctgcaaca ggcgaaagcg cgtgatgtgc
    26881 agaattttta cttcttctgc cagcacatta cgcttatccc gacgttgcgc agtttgctgg
    26941 aacagccgga taacggtatc gatgcgttcc tcgcgccggg tcacgtcagt atggttatcg
    27001 gcaccgacgc ctataatttt atcgccagcg attttcatcg tccgctggtg gttgctggat
    27061 tcgaacccct tgatctacta caaggcgtgg tcatgctggt gcagcagaaa atagcggccc
    27121 acagcaaggt agagaatcag tatcgtcgag tggtaccgga tgccggtaac ctgctggcgc
    27181 aacaggcgat tgccgatgtg ttctgtgtca acggcgacag cgaatggcgc ggcttaggcg
    27241 tgattgaatc ttctggcgtg cacctgacgc cggattatca acgattcgat gccgaagcac
    27301 atttccgccc ggcaccgcag caggtctgcg atgacccgcg cgcgcgttgt ggtgaggtat
    27361 taacgggcaa atgtaagccg catcaatgcc cgctgtttgg taacacctgt aatcctcaaa
    27421 ccgcgtttgg tgcgctgatg gtttcctccg aaggagcgtg cgccgcgtgg tatcagtatc
    27481 gtcagcagga gagtgaagcg tgaataatat ccaactcgcc cacggtagcg gcggccaggc
    27541 gatgcagcaa ttaatcaaca gcctgtttat ggaagccttt gccaacccgt ggctggcaga
    27601 gcaggaagat caggcacgtc ttgatctggc gcagctggta gcggaaggcg accgtctggc
    27661 gttctccacc gacagttacg ttattgaccc gctgttcttc cctggcggta atatcggcaa
    27721 gctggcgatt tgcggcacag ccaatgacgt tgcggtcagt ggcgctattc cgcgctatct
    27781 ctcctgtggc tttatcctcg aagaaggatt gccgatggag acactgaaag ccgtagtgac
    27841 cagcatggca gaaaccgccc gcgcggcagg cattgccatc gttactggcg atactaaagt
    27901 ggtgcagcgc ggcgcggtag ataaactgtt tatcaacacc gctggcatgg gcgcaattcc
    27961 ggcgaatatt cactggggcg cacagacgct aaccgcaggc gatgtattgc tggtgagcgg
    28021 tacactcggc gaccacgggg cgactatcct taacctgcgt gagcagctgg ggctggatgg
    28081 cgaactggtc agcgactgcg cggtgctgac gccgcttatt cagacgctgc gtgacattcc
    28141 cggcgtgaaa gcgctgcgtg atgccacccg tggtggtgta aacgcggtgg ttcatgagtt
    28201 cgcggcagcc tgcggttgtg gtattgaact ttcagaagcg gcactgcctg ttaaacctgc
    28261 cgtgcgtggc gtttgcgaat tgctgggact ggacgccctg aactttgcca acgaaggcaa
    28321 actagtaata gctgttgaac gcaacgcggc agagcaagtg ctggcagcgt tacattccca
    28381 tccactgggg aaagacgcgg cgctgattgg tgaagtggtg gaacgtaaag gtgttcgtct
    28441 tgccggtctg tatggcgtga aacgaaccct cgatttacca cacgccgaac cgcttccgcg
    28501 tatatgctaa taaaattcta aatctcctat agttagtcaa tgaccttttg caccgctttg
    28561 cggtgctttc ctggaagaac aaaatgtcat atacaccgat gagtgatctc ggacaacaag
    28621 ggttgttcga catcactcgg acactattgc agcagcccga tctggcctcg ctgtgtgagg
    28681 ctctttcgca actggtaaag cgttctgcgc tcgccgacaa cgcggctatt gtgttgtggc
    28741 aagcgcagac tcaacgtgcg tcttattacg cgtcgcgtga aaaagacacc cccattaaat
    28801 atgaagacga aactgttctg gcacacggtc cggtacgcag cattttgtcg cgccctgata
    28861 cgctgcattg cagttacgaa gaattttgtg aaacctggcc gcagctggac gcaggtgggc
    28921 tatacccaaa atttggtcac tattgcctga tgccactggc ggcggaaggg catatttttg
    28981 gtggctgtga atttattcgt tatgacgatc gcccctggag cgaaaaagag ttcaatcgtc
    29041 tgcaaacatt tacgcagatc gtttctgtcg tcaccgaaca aatccagagc cgcgtcgtta
    29101 acaatgtcga ctatgagttg ttatgccggg aacgcgataa cttccgcatc ctggtcgcca
    29161 tcaccaacgc ggtgctttcc cgcctggata tggacgaact ggtcagcgaa gtcgccaaag
    29221 aaatccatta ctatttcgac attgacgata tcagtatcgt cttacgcagc caccgtaaaa
    29281 acaaactcaa catctactcc actcactatc ttgataaaca gcatcccgcc cacgaacaga
    29341 gcgaagtcga tgaagccgga accctcaccg aacgcgtgtt caaaagtaaa gagatgctgc
    29401 tgatcaatct ccacgagcgg gacgatttag ccccctatga acgcatgttg ttcgacacct
    29461 ggggcaacca gattcaaacc ttgtgcctgt taccgctgat gtctggcgac accatgctgg
    29521 gcgtgctgaa actggcgcaa tgcgaagaga aagtgtttac cactaccaat ctgaatttac
    29581 tgcgccagat tgccgaacgt gtggcaatcg ctgtcgataa cgccctcgcc tatcaggaaa
    29641 tccatcgtct gaaagaacgg ctggttgatg aaaacctcgc cctgaccgag cagctcaaca
    29701 atgttgatag tgaatttggc gagattattg gccgcagcga agccatgtac agcgtgctta
    29761 aacaagttga aatggtggcg caaagtgaca gtaccgtgct gatcctcggt gaaactggca
    29821 cgggtaaaga gctgattgcc cgtgcgatcc ataatctcag tgggcgtaat aatcgccgca
    29881 tggtcaaaat gaactgcgcg gcgatgcctg ccggattgct ggaaagcgat ctgtttggtc
    29941 atgagcgtgg ggcttttacc ggtgccagcg cccagcgtat cggtcgtttt gaactggcgg
    30001 ataaaagctc cctgttcctc gacgaagtgg gcgatatgcc actggagtta cagccgaagt
    30061 tgctgcgtgt attgcaggaa caggagtttg aacgtctcgg cagcaacaaa atcattcaga
    30121 cggacgtgcg tctaatcgcc gcgactaacc gcgatctgaa aaaaatggtc gccgaccgtg
    30181 agttccgtag cgatctctat taccgcctga acgtattccc gattcacctg ccgccactac
    30241 gcgagcgtcc ggaagatatt ccgctgctgg cgaaagcctt taccttcaaa attgcccgtc
    30301 gtctggggcg caatatcgac agcattcctg ccgagacgct gcgcaccttg agcaacatgg
    30361 agtggccggg taacgtacgc gaactggaaa acgtcattga gcgcgcggta ttgctaacac
    30421 gcggtaacgt gctgcagctg tcattgccag atattgtttt accggaacct gaaacgccgc
    30481 ctgccgcaac ggttgtcgcc ctggagggcg aagatgaata tcagttgatt gtgcgcgtgc
    30541 tgaaagaaac caacggcgtg gttgccgggc ctaaaggcgc tgcgcaacgt ctggggctga
    30601 aacgcacgac cctgctgtca cggatgaagc ggctgggaat tgataaatcg gcattgattt
    30661 aactgcaaat tgccggacag atctgcctgt ccggcatact attcatgagg ttttttcgga
    30721 cgatattttt ccggcagttc tggcaccgga cgcttgtcat cgatgagatg acgcacggtt
    30781 aagatcggat gacgccacag cattctcggc ccggcccaac gcataatctg tttcatctct
    30841 tcacgctttg caggctggta acagtgcacc ggacactgct tacaggctgg tttctcttcg
    30901 ccgaacacac atttatccag ccgcttttgc gcgtaaacaa acaacgcctc gtaatgctcc
    30961 ggctccgctg acgcctgcgg gcatttcgct tgataaagat cgatcatttt tttaatcgtc
    31021 agtttttcac gagagatacg cttgccggac atgctgcctc cacctcatta agatgtattt
    31081 atattacatc ttaatcttaa agggcactat gactccaaag aagaagggtt agccaaccga
    31141 tacaattttg cgtacttgct tcataagcat cacgcaaaag ctgcaaaaca gcatctttcc
    31201 cggaaccagc atcaagaact cgccgttcgc ttcttcccct gaaatgatta actccggtat
    31261 catgtgcgcc ttatgtgatt acaacgaaaa taaaaaccat cacaccccat ttaatatcag
    31321 ggaaccggac ataaccccat gagtgcaata gaaaatttcg acgcccatac gcccatgatg
    31381 cagcagtatc tcaggctgaa agcccagcat cccgagatcc tgctgtttta ccggatgggt
    31441 gatttttatg aactgtttta tgacgacgca aaacgcgcgt cgcaactgct ggatatttca
    31501 ctgaccaaac gcggtgcttc ggcgggagag ccgatcccga tggcggggat tccctaccat
    31561 gcggtggaaa actatctcgc caaactggtg aatcagggag agtccgttgc catctgcgaa
    31621 caaattggag atccggcgac cagcaaaggt ccggttgagc gcaaagttgt gcgtatcgtt
    31681 acgccaggca ccatcagcga tgaagccctg ttgcaggagc gtcaggacaa cctgctggcg
    31741 gctatctggc aggacagcaa aggtttcggc tacgcgacgc tggatatcag ttccgggcgt
    31801 tttcgcctga gcgaaccggc tgaccgcgaa acgatggcgg cagaactgca acgcactaat
    31861 cctgcggaac tgctgtatgc agaagatttt gctgaaatgt cgttaattga aggccgtcgc
    31921 ggcctgcgcc gtcgcccgct gtgggagttt gaaatcgaca ccgcgcgcca gcagttgaat
    31981 ctgcaatttg ggacccgcga tctggtcggt tttggcgtcg agaacgcgcc gcgcggactt
    32041 tgtgctgccg gttgtctgtt gcagtatgcg aaagataccc aacgtacgac tctgccgcat
    32101 attcgttcca tcaccatgga acgtgagcag gacagcatca ttatggatgc cgcgacgcgt
    32161 cgtaatctgg aaatcaccca gaacctggcg ggtggtgcgg aaaatacgct ggcttctgtg
    32221 ctcgactgca ccgtcacgcc gatgggcagc cgtatgctga aacgctggct gcatatgcca
    32281 gtgcgcgata cccgcgtgtt gcttgagcgc cagcaaacta ttggcgcatt gcaggatttc
    32341 accgccgggc tacagccggt actgcgtcag gtcggcgacc tggaacgtat tctggcacgt
    32401 ctggctttac gaactgctcg cccacgcgat ctggcccgta tgcgccacgc tttccagcaa
    32461 ctgccggagc tgcgtgcgca gttagaaact gtcgatagtg caccggtaca ggcgctacgt
    32521 gagaagatgg gcgagtttgc cgagctgcgc gatctgctgg agcgagcaat catcgacaca
    32581 ccgccggtgc tggtacgcga cggtggtgtt atcgcatcgg gctataacga agagctggat
    32641 gagtggcgcg cgctggctga cggcgcgacc gattatctgg agcgtctgga agtccgcgag
    32701 cgtgaacgta ccggcctgga cacgctgaaa gttggcttta atgcggtgca cggctactac
    32761 attcaaatca gccgtgggca aagccatctg gcacccatca actacatgcg tcgccagacg
    32821 ctgaaaaacg ccgagcgcta catcattcca gagctaaaag agtacgaaga taaagttctc
    32881 acctcaaaag gcaaagcact ggcactggaa aaacagcttt atgaagagct gttcgacctg
    32941 ctgttgccgc atctggaagc gttgcaacag agcgcgagcg cgctggcgga actcgacgtg
    33001 ctggttaacc tggcggaacg ggcctatacc ctgaactaca cctgcccgac cttcattgat
    33061 aaaccgggca ttcgcattac cgaaggtcgc catccggtag ttgaacaagt actgaatgag
    33121 ccatttatcg ctaacccgct gaatctgtcg ccgcagcgcc gtatgttgat catcaccggt
    33181 ccgaacatgg gcggtaaaag tacctatatg cgccagaccg cgttgattgc gctgatggcc
    33241 tatatcggca gctacgtacc ggcgcaaaaa gtcgagattg gcccgattga ccgtatcttt
    33301 acccgcgtag gcgcggcaga tgacctggct tccgggcgct caacctttat ggtggagatg
    33361 actgaaaccg ccaatatttt acataacgcc accgagtaca gtctggtgct gatggatgag
    33421 attgggcgcg gaacttccac ttacgatggt ctgtcgctgg cgtgggcgtg cgcggaaaat
    33481 ctggcgaata agattaaagc gttgacgctg tttgccaccc actatttcga gctgacacag
    33541 ttaccggaga aaatggaagg cgtcgccaac gtgcatctcg atgcactgga gcacggcgac
    33601 accattgcct ttatgcacag cgtgcaggat ggcgcggcga gcaaaagcta cggcctggcg
    33661 gttgcagctc tggcaggcgt gccaaaagag gtgattaagc gcgcacggca aaaactgcgt
    33721 gagctggaaa gcatttcgcc gaacgccgcc gctacgcaag tggatggtac gcaaatgtct
    33781 ttgctgtcag taccggaaga aacctcgcca gcggtcgaag ctctggaaaa cctggacccg
    33841 gattcactga ctccgcgtca ggcgctggaa tggatttatc gcttgaagag cctggtgtaa
    33901 taacaattcc cgatagtctt ttgctatcgg gaatattaac gacaactgac gaataaaata
    33961 aaaacaccct gtataatagg aaagcttatt ttacagggta aaaccatgcc atctacacgc
    34021 tatcaaaaaa ttaatgccca tcactatcgc catatatggg tcgttggtga tattcatggt
    34081 gaatatcaat tattacaatc ccgcttacat caactctctt tttgccctga aaccgactta
    34141 cttatttcgg taggcgataa catcgatcgt gggccggaga gtcttaacgt gctgcgcctg
    34201 ctaaaccagc cctggtttac gccggttaaa ggcaaccacg aagcaatggc gctggatgcg
    34261 ttcgcgaccg gcgatggaaa tatgtggctt gccagcggtg gtgactggtt tttcgattta
    34321 aatgattcag agcaacaaga ggcaatagat ctgttgctga aattccatca ccttccacat
    34381 attattgaaa tcattaatga caacataaaa tatgtcatcg cacatgcaga ttatccgggt
    34441 gatgaatatc agtttggtaa agaaatagta gaaagcgaat tactctggcc tgttgatcgt
    34501 gtgcagaaat cgcttaatgg cgagttacaa caaataaacg gcgctgatta ttttatattt
    34561 ggacatatga tgtttgataa cattcagacg ttcgctaacc agatttatat tgataccggc
    34621 acgccgaaaa gcgggcggct gtcattttat aaaataaagt agtctcatgc ttcttctgtg
    34681 aagcatgagt aaccaaatgt tattgcaggc cattattcat ttttcgctac cagcaaagag
    34741 agatcctgct tcgccagcgc gcgactggca ctctccggca aaccgtcgtc tgtgataatc
    34801 tgatcaaact cgcttaatgg taacgccagc catgtcgcca cctgaccata tttcgtcgca
    34861 tcacagacca aaactcgctg gcggctggca ctggcaatcg cccgtttcac agtgacttta
    34921 tcttccgctg gcgtagaaat cccccgcaca ctccatgacg atgcagaaat aaaagcttga
    34981 tcaatcatca ggctgcgcag catggtcgca gcggcttccc cgacacagga acggttttcc
    35041 cgacacactg caccgccagt gtgaataatt gtgcaattac tgttgtcgag caagtagtct
    35101 gcaataacga aatcgtttgt gaccacagtc agtgactcca tgtgaatcag atgctgtgct
    35161 atcgctaacg tggtcgttcc cgcatccaga tagatacaac ttcccggctg aacaagactt
    35221 gccgccagct tgccaatagc cgctttttgc gtcattgcca gcgcagtttt tacctgatga
    35281 gaaggttcat gcgccacgcg tcccggagac tggacgcctc cggacaccag cacaacggct
    35341 ccctgctgct ccagtttttg taaatcccga cgaatggtca tatgtgacac attcattctg
    35401 tccgttagtt cagcaatact gacaatgcct ttttcagcta ccatctcaag gatgatttgg
    35461 cgacgctcta cgggtatcaa cttttgctcc ttcctttgtc ctgctgacat tctacgctat
    35521 ttgcctgcga aacgtgcgcg gcgcaactaa cgcttagttc acataaaata acacacaatg
    35581 ttaatttatg tgaatcagct caccatatcg ttatcttcca gcgcttatat tcacaatatc
    35641 aaacaaaata tcacttaaat taacaaggag agcagatgaa aacgggatct gagtttcatg
    35701 tcggtatcgt tggcttaggg tcaatgggaa tgggagcagc actgtcatgt gtccgcgcag
    35761 gtctttctac ctggggcgca gacctgaaca gcaatgcctg cgctacgttg aaagaggcag
    35821 gtgcttgcgg ggtttctgat aacgccgcga cgtttgccga aaaactggac gctctgctgg
    35881 tgctggtggt caatgcggcc caggttaaac aggtgctgtt tggtgaaaca ggcgttgcac
    35941 aacatctgaa acccggtacg gcagtaatgg tttcttccac tatcgctagt gctgatgcgc
    36001 aagaaattgc tatcgctctg gctggattcg atctggaaat gctggatgcg ccagtttctg
    36061 gtggtgcagt aaaagccgct aacggtgaaa tgactatcat ggcctccggt agcgatattg
    36121 cctttgaacg actggcaccc gtgctggaag ccgttgccgg aaaagtttat cgcataggtg
    36181 cagaaccggg actaggttcg accgtaaaaa ttattcacca gttgttagcg ggcgtacata
    36241 ttgctgccgg agccgaagcg atggcacttg cagcccgtgc ggggatcccg ctggatgtga
    36301 tgtatgacgt cgtgaccaat gccgccggaa attcctggat gttcgaaaac cggatgcgtc
    36361 atgtggtgga tggcgattac accccgcatt cagccgtcga tatttttgtt aaggatcttg
    36421 gtctggttgc cgatacagcc aaagccctgc acttcccgct gccattggcc tcaacagcat
    36481 tgaatatgtt caccagcgcc agtaacgcgg gttacgggaa agaagacgat agcgcagtta
    36541 tcaagatttt ctctggcatc actctaccgg gagcgaaatc atgatcaaga ttggcgttat
    36601 cgccgatgat tttaccggcg cgacggatat cgccagtttt ctggtggaaa acggtctacc
    36661 aacggtacaa attaacggtg ttccaacagg taaaatgccg gaagcaatcg acgcactggt
    36721 gatcagcctg aaaacgcgct cctgtccagt ggttgaagcc acacagcaat cgctggcggc
    36781 tctgagctgg ttgcaacagc aaggttgcaa acagatctat ttcaaatact gctctacttt
    36841 cgacagtacg gcgaaaggta atattggccc ggttaccgat gccttaatgg atgctctcga
    36901 cacgccgttt acggtcttct ctccggccct gccggtcaac ggacgtacgg tttatcaggg
    36961 gtatttgttc gtaatgaatc aactgctggc cgaatccggg atgcgccatc acccggtaaa
    37021 tcccatgacc gacagctatc ttccccgtct ggttgaagcg caatccacag ggcgctgcgg
    37081 cgtcgtttcg gcacatgttt tcgaacaagg tgtggatgcc gttcgtcaag agctggctcg
    37141 cttacagcaa gagggctacc gctacgcggt gcttgatgcg ctgaccgaac accatctgga
    37201 aattcaggga gaagccttgc gcgatgcccc actggtaacg ggcggttctg gtctggcgat
    37261 tggcctggcc cggcagtggg cgcaagaaaa cggtaaccag gctcgcaaag cagggcgtcc
    37321 gctcgctggg cgcggcgtag tgctctccgg ttcatgctct caaatgacca accgccaggt
    37381 agcacattac cgtcaaattg caccagcccg tgaagttgat gtggcacgct gcctctcaat
    37441 tgaaactctg gccgcttatg cacacgaact ggcagagtgg gttctgggcc aggaaagtgt
    37501 acttgctcca ctggtttttg ccaccgccag cactgacgca ttggcagcaa ttcaacagca
    37561 atacggtgca caaaaagcca gtcaggcagt agaaacactg ttttctcaac tagcggcgcg
    37621 gttagcagcg gaaggcgtga cacgctttat tgtcgcaggc ggtgagacct ccggcgtagt
    37681 cacacagagc ctgggaataa aagggtttca tattggccca accatttccc ccggcgtgcc
    37741 gtgggtaaac gcactggata agcctgtctc actcgccctt aaatctggca acttcggtga
    37801 tgacgccttt ttttcacgag cccaaagaga gtttttatca tgagcgattt cgcaaaagta
    37861 gagcagtctt tgcgagagga gatgacgcgg attgccagtt cattctttca gcgcggctat
    37921 gcaaccggtt cggctggcaa tctgtcgctg cttttacctg acgggaattt actggcgaca
    37981 ccgacaggtt catgcctggg caatctcgat ccgcagcggc tttccaaagt cgccgcggat
    38041 ggcgaatggt taagtggtga caaaccctcg aaagaggtgc tctttcatct ggcgctgtat
    38101 cgcaacaatc cgcgttgtaa agcggtggtg catttgcaca gcacatggtc gacggcgctt
    38161 tcctgcctgc aagggctgga cagcagcaac gttattcgtc cgttcacacc atacgtggtg
    38221 atgcggatgg gaaatgtccc gctggtgcct tattaccgac cgggcgataa acgcatcgca
    38281 caggatctgg cggaactggc agcagacaat caggcttttt tactggcaaa tcatggccca
    38341 gtggtttgcg gtgaaagcct gcaagaagcc gccaacaata tggaagagct ggaggaaacg
    38401 gcaaagctga tttttattct cggtgaccgc ccgatccgtt atctgaccgc aggtgaaatt
    38461 gcggaattaa ggagttaatg caatgcctcg ttttgcagct aatttatcca tgatgttcac
    38521 cgaagtgcct tttattgaac gcttcgccgc agcgcgaaaa gccgggttcg atgctgtgga
    38581 atttctgttt ccctatgact actccaccct gcaaatccaa aagcaactgg agcaaaacca
    38641 tctgacactg gcgctgttca ataccgctcc cggagatatt aatgcggggg aatggggatt
    38701 atccgccctt cccggacgtg agcacgaagc acacgcagac atcgacctgg cactggaata
    38761 tgcattggcg ctcaattgtg aacaagtcca tgtgatggca ggcgtcgtgc ccgctggcga
    38821 agatgctgaa cggtaccggg cagtatttat cgataatctc cgctacgctg ccgaccgctt
    38881 tgcgccacac ggcaagcgaa tattagttga agcactcagc cccggcgtta agcctcatta
    38941 tctcttctcc agccagtatc aggcactggc tattgttgaa gaagttgcgc gagataacgt
    39001 gtttattcaa ctagatactt ttcatgcgca aaaagtagat ggcaacctga cgcatttaat
    39061 ccgcgactac gccgggaaat atgcgcacgt acaaattgcc ggactacctg atcgacatga
    39121 accggacgat ggagaaatca actatccgtg gctgttccgc ctgttcgatg aggtgggata
    39181 tcagggctgg atcggttgtg aatataaacc tcgtggcctc accgaagaag ggcttggctg
    39241 gtttgacgcc tggcgctaat tcgttaaatc cctaattaca acgtacccat acatcccccc
    39301 atctacggat ggggtaggtt aactataact ttcagacagg gtttcccatg tccacaatta
    39361 cattgttatg cattgcgtta actggcgtaa tcatgctgtt gctgctggtc atcaaggcaa
    39421 aggtacaacc attcgttgct ctgctcctcg tcagcctgtt agtcgcactt gcggcgggta
    39481 taccggcggg cgaagtgggt aaagtgatga tcgccgggat gggcggtgtg cttggttccg
    39541 tcactattat tattggtctg ggcgctatgc tggggcgtat gatcgaacac tctggtggtg
    39601 cagagtcact ggctaattat ttcagtcgca agttaggtga caaacgaact atcgctgcgc
    39661 tgactctggc agcgttcttc ctcggtattc ccgtcttctt tgatgtcggc tttattattc
    39721 ttgcgccaat catttacggt tttgccaagg ttgccaaaat atcgccactc aaatttggcc
    39781 tgcctgtcgc tgggatcatg ctcactgttc acgtggcggt accgccgcat ccaggccctg
    39841 tcgccgcagc ggggttactc cacgcagaca tcggctggct aaccatcatc ggtattgcga
    39901 tttctattcc cgtaggggtt gttggctact ttgcagcgaa aataatcaat aagcgccaat
    39961 atgcgatgtc agtagaagta ctggaacaga tgcaactggc tccggccagt gaggaaggcg
    40021 cgacaaaatt aagcgataaa ataaatccac cgggcgtcgc gctggtcacc tcgctaattg
    40081 ttattcctat cgcgattatc atggcgggta cggtttccgc aacactgatg ccgccttcgc
    40141 atcccctgct tggtacgcta cagctgatcg gctcaccaat ggtagctcta atgattgcgc
    40201 tggtgctggc attctggtta ttggctttac gtcgcggctg gagcttacaa cacaccagcg
    40261 acattatggg ctcagccctt cccactgcgg cggtagtgat tttggttact ggtgctggag
    40321 gggtatttgg caaagtgctg gtggaatcgg gcgtcggcaa agcccttgcc aacatgctac
    40381 aaatgattga cctgcctctg ttaccagccg catttattat ttcactggcg ctgcgtgcat
    40441 cgcaggggtc agcaaccgta gcaatcctga caaccggcgg gttactctca gaagcggtga
    40501 tgggattgaa tccgattcag tgcgtattgg tgacgctggc agcctgcttt ggtgggcttg
    40561 gcgcgtcaca tattaatgac tcagggttct ggattgtgac caaatatctg gggttgtcgg
    40621 tagcagacgg tctgaaaacc tggacagtgt taacgaccat tctcggtttt accggattct
    40681 taattacctg gtgcgtatgg gcggtaattt gaccttaata aaaaggtccg atgggcatcg
    40741 gaccttttat tgtgcacaga aaaggccagc ctcgcttgag actggccttt ctgacagatg
    40801 cttacttact cgcggaacag cgcttcgata ttcagcccct gcgtttgcag gatttcacgc
    40861 aaacggcgca ggccttcaac ctgaatctgg cgaacacgtt cacgggtgag gccaatttca
    40921 cgacctacat cttccagtgt tgccgcttcg taccccagca aaccgaatcg acgtgccagc
    40981 acttcacgct gtttggcgtt cagctcgaac agccatttga cgatgctctg cttcatatcg
    41041 tcatcttgcg tggtatcttc cggaccgttc tctttttcat cggccaggat gtccagcaac
    41101 gctttttcgg aatcaccacc cagcggggtg tctaccgagg taatgcgctc gttaagacga
    41161 agcatacggc tgacgtcatc aactggctta tccagttgct ctgcgatctc ttccgcactt
    41221 ggttcatggt ccagcttatg ggacaactca cgtgcggttc gcaggtaaac gttcagctcc
    41281 tttacgatgt gaatcggcaa acgaatagta cgggtttggt tcataatcgc ccgttcaatc
    41341 gtctggcgaa tccaccaggt tgcgtatgtt gagaagcgga aaccacgttc cgggtcaaac
    41401 ttctctaccg cgcggatcag ccccaggttg ccctcttcga taaggtccag caacgccaga
    41461 ccacgattgc cataacggcg ggcaattttt accaccagac gcaagttact ctcgatcatc
    41521 cggcggcgag aggcgacatc tccacgcagt gcgcgacgcg caaaataaac ttcttcttcg
    41581 gccgttaaca gtggtgaata accaatctca ccaaggtaaa gctgagtcgc gtccaacaca
    41641 cgctgtgtgg ctccctgcga taacagttcc tcttcggcca aatcgttatc actgggttcc
    41701 tcttctacta aggccttttc gtcaaaaacc tcaactccgt tctcatcaaa ttccgcatct
    41761 tcatttaaat catgaacttt cagcgtattc tgactcataa ggtggctcct acccgtgatc
    41821 ccttgacgga acattcaagc aaaagcctgg ttccgccgat ttatcgctgc ggcaaataac
    41881 gcagcgggtt tacggatttc cccttgtaac gaatttcaaa atgcaagcgt gttgaactgg
    41941 ttccggtgct acccatggtt gctatttttt gccccgcctt cacttcttgt tgttcccgga
    42001 ccagcattgt gtcgttatgg gcgtaggcac tcaggtaatc atcattatgt ttgatgataa
    42061 tcagattacc gtagccgcgc agcgcgttac cagcataaac aacgcggcca tctgcggtcg
    42121 cgataattgc ctgtcctttg ctgcctgcga tatcaatccc cttgttgccc ccctcagaag
    42181 cgccaaaggt ttcgatcact ttgccctcag tcggccagcg ccaggtggag ataggcgtac
    42241 tggttgatgt actgctgaca gttggctcgg ttgtgcttgc tgttggtacc gttacaggcg
    42301 ctgtgaccgt ggtcacagct ggcttgttgt tcggcaacat tttgttagca ctctgttcac
    42361 ccgaagattc agaatacgta attgtcggtt gcgacgcaac agcaacggtg gaattttgtg
    42421 caggcttgat cacaactcct tgctctgctg cgtcggcctg ggtaatggca tttccgccag
    42481 tgattggcgt accggaagca ttacccacct gcaaggtctg accaacgttc agcgcgtatg
    42541 gtgcctgaat attgttgcgc tgagcaaggt cacggaaatc gttgccagta atccaggcga
    42601 tatagaaaag tgtgtcgcct tttttcacgg tataggtact gccgctataa ctgcctttcg
    42661 gaatgttccc atactgacgg ttatagacga tgcgtccgtt ttccatctgt accggctgct
    42721 gagctactgg ctgcactggc tggatttgcg gttgttgagt agcctgaatt tgtggctgct
    42781 gtaccggctg aatttgcggt tgctgcgctg tagacgtcgt ccccattttc ggcggcggcg
    42841 taatcaacat accagaatta gtatttgcag gcgcattgcc attaacggag ctgaccggtg
    42901 ccggtggatt tgaagtgtca gaacagcctg ccagccatag cgaaaccagt gacaaagccg
    42961 caatgcggcg aacggtgaat tttgggcttc ccgcgctcat ttatccccca ggaaaaattg
    43021 gttcataacc agtgacataa ttaccgtgca aggcacccta ctgaacactg gaaaagatgt
    43081 tcacgatacg ctgacctgcg gcaaaataac caggaaaaat ccaggtattt cctcacgttt
    43141 taagccagct cacccttcac taaagggaca aagcgcacgg cctccacggt atcgataata
    43201 aattcgcctc cccgacgacg cacccgtttc aaatactggt gctcctcccc tacgggtaag
    43261 acgagaatcc cgccttcgtc cagctgcgtc attagcgcag ttggaatttc cggcggtgcc
    43321 gccgtaacaa tgatagcgtc aaacggcgca cgtgcctgcc aaccttgcca tccatcgcca
    43381 tgacgggttg aaacattatg taaatcaaga tttttcaggc ggcgacgtgc ctgccactgc
    43441 aagcctttaa tccgttcaac cgagcaaaca tgctggacaa gatgcgccag gattgccgtt
    43501 tgatatcccg aaccggtgcc aatttccagc acccgcgact gcggcgtcag ctcgagtaat
    43561 tcggtcattc gcgccaccat atatggctgc gaaattgtct gcccctgacc tatcggcaag
    43621 gcgatattgt cccaggcttt ttgttcaaac gcttcatcaa cgaatttttc acgcggcacg
    43681 gcggcaagtg cattcagcac ctgctcatcc tgaatacctt gcgcacgtaa ttgatccaga
    43741 agtgcttgta cgcgtctgct taccattgcg tgccaactcc cacgctgttt aaccagtctg
    43801 aaaccacatc ttgcgcgcta tgcgcagtta aatccacatg cagcggcgtg atggagacat
    43861 agccctcatc taccgcagca aaatcggtcc ccggaccggc atcacattta ccgcccggcg
    43921 ggccaatcca gtacagcgta ttgccgcgcg gatcttgctg cgggatcacc tgatctgccg
    43981 gatgtcgtgt accgcagcgc gtcacgcgaa tacctttgat ttgatccaag ggtaaatccg
    44041 gaacgttaat attaagaata cgcccggtgc gcagcggctc tttacacagt gcgcgcaaaa
    44101 ttgaacaggt taccgccgcg gcagtgtcgt aatgtttatg cccgtcaagc gagacggcaa
    44161 gcgccggaaa acctaaatga cggccttcca tcgcggcggc taccgtaccg gaataaataa
    44221 catcatcccc cagattcggc ccggcgttaa ttccggacac aacaatgtcc gggcgcggac
    44281 gcatcagagc attcacgcca agatagacgc aatcggtcgg ggttcccatt tgcacagcaa
    44341 tatcaccatt ttcaaaggta aacgtgcgca gggaggattc cagtgtcaga gaatttgaag
    44401 cgccgctgcg gttacgatcg ggggcgacca cctgaacgtc agcaaactca cgcaaggctt
    44461 tcgccagcgt ttgtataccg ggtgcatgta ccccgtcatc attactcagc aatatgcgca
    44521 taatcacctg ttgtgttgat aagttccctg acaacgctgg ttgcaaaact acccgccgga
    44581 agccagaaac ggatctctac ggtgacgtca tcccaccaat tccagcttaa ttgttgcgga
    44641 tacagcagca tcgctctgcg cgcggcttca actttttcgc gcaccagtaa agcttgtaat
    44701 tcagtttctg cggcgacagc cgcttgttcg aatgccagcg cttcacgctg agttccccat
    44761 tcgccactgc ccggcaatgc ggcggttatc atcagctctt tatcgttaac gcgacgctgt
    44821 aattccgcca gttcttcggt ggttgcgaca aaccagctac cacgtccggc taattgtagc
    44881 gcatcgccgt caacaacttg attaacgtct gcttttttga ggcgctcagc aacaatctga
    44941 ttaaacaacg cactgcgggc tgccgacaac caaaaactcc gtttattgcg atcgcgcacc
    45001 ggagtattgg tttgcgccca gcgctgcgcc ccctgcaagt tgctaccgcc aatcccaaaa
    45061 cgttgggcac cgaagtagtt cggtacacct tttacgcaaa tatcgttcag acgttgttca
    45121 acgtcatcgc gattgctcac ttcgcgcaga accagggtaa aggcgtttcc tttcagcgcc
    45181 cctaaacgca gcttgcgctt gtgccgcgca tactccagca cctggcagcc ttccagttga
    45241 aaggcgctca gatcgggcat ttccttgccc ggcacgcgag cgcataacca ctgttccgta
    45301 acagcatgtt tgtctttttg cccagcgaag ctgacttcac gggcatgaat tttcaggaat
    45361 ttcgccagtg catccgccac aaaacgggta ttgcagccgt ttttgaggat tctaaccaga
    45421 atatgctcac cttcaccatc aggctcaaag cccaaatctt ccaccaccac aaagtcttcc
    45481 ggattggctt tcagcagccc ggtgccttgc ggtttaccgt ggaggtaagt gagattatca
    45541 aactcaatca ttttgttgcc ttaatgagta gcgccaccgc ttcacaggca atcccttccc
    45601 cacgtccggt aaatcccatt ttttccgtag tagtggcttt cacgttaaca tcatccatat
    45661 ggcagccgag atcttcggca ataaacacgc gcatttgtgg aatgtgcggc aacatcttcg
    45721 gtgcctgagc gatgatagtg acatcgacgt tgccaagggt ataacccttc gcctgaatac
    45781 gacgccaggc ttcgcgtagc agctcgcggc tatcggcacc tttaaatgcc ggatcggtat
    45841 ccgggaacag cttgccgata tcccccagcg ccgccgcgcc aagcaatgca tcggtcaacg
    45901 catggagcgc cacgtcgcca tcagaatgcg ccagcaatcc tttttcgtaa ggaatgcgta
    45961 cgccaccaat gataattggg ccttcaccgc caaaggcatg tacgtcaaaa ccgtgtccaa
    46021 ttcgcattat gtattctcct gatggatggt tcgggtgagg taaaactcgg ccagtgccaa
    46081 atcttccggg cgcgtgactt taatgttatc cgcacggcct tcgaccaact gaggatggaa
    46141 tccgcaatat tccagcgccg aggcttcgtc ggtaatagtc gcgccttcat ttagagcgcg
    46201 cgtcagacag tcatgtaaca gctcacgagg gaaaaattgc ggcgtcagcg cgtgccataa
    46261 gccgttgcga tcaacggtat gagcaatggc atttttgccc ggttcggcac gtttcatagt
    46321 atcgcgcact ggtgcggcga ggatcccccc cgtgcggctg gtttcgctca acgccaacaa
    46381 tcgcgcgagg tcatcctgat gcaaacaagg acgagcggcg tcatgcacca atacccactg
    46441 cgcgtcgcca gcggctttca gacctgccag cacggaatcg gcacgctcat caccgccatc
    46501 tacaacggtg atttgcggat gattcgccag aggaagttgt gcaaaacggc tatcgccagg
    46561 acttatggca atgacgactc gtttcacccg gggatgcgcc agcagcgcat gcaccgagtg
    46621 ttcaagaatg gtttgattac cgattgagag atattgctta ggacattccg tttgcattcg
    46681 acggccaaat ccggccgccg gaaccacggc gcaaacatcc aaatgagtgg ttgccatgtt
    46741 aattcccggg ctgatttatc gattgttttg ccccgcagac tgtgcgcgct tcgacgcgtc
    46801 aggcaccaga cgataaaaag tttcgcccgg cctggtcatg ctgagttcat tacgcgcacg
    46861 ctcttcgagc gcctcctggc cgccattgag atcgtcaatt tcggcaaaaa gttgatcgtt
    46921 tcgcgcttta agtttcgcgt ttgtagcttg ctgtgccgcc acatcatcat tgacgcgggt
    46981 atagtcatgt ataccgttct taccgaacca cagcgaatac tgtagccaga ccagaatagc
    47041 cagcaacagc agcgttagtt tacccatcct gccccctgaa aaacggcatc atcatcccat
    47101 gcatccgaag acgactctac atcctctgtt ggggataccg cgacaacgcg ggcaaatgta
    47161 ccacatttgt ccattgttac gtatacccag ggcgtgcaga acataatctc attattagtt
    47221 acggtttgaa ttatgaacag aggagacggg aaagtacaaa ttagcccagt agccacataa
    47281 acagtgcgcc aaacataatg cctactgtca ccagggtgaa aacaatactg tagcgtagct
    47341 ttccgtccat caatgaatgc agcgcaatcc ccaccactac cgcgacgggc atcagcgcaa
    47401 gaaagaaagg ccaggtgtag ataaagaaga acagcgtgtt agagccataa atcaacatcg
    47461 gcatcgccag cgcaaataac caggagataa aaccgaccac ggcaccaggc agtgaccatg
    47521 tggtttcttc atcctcagta aggctgtcgt tatttgttag tgtaatgtta tggctattac
    47581 gcatatttga tcctgttact ttgacgaacc gggcatggaa acccggtggt gtctcaggat
    47641 ctgataatat cgttctgtct caacagatct aataattgct gtaccaaatt tgttactaat
    47701 tgttcaccat tgagatgaat ttctgccgat tcaggcgctt cgtaaacgga atctattccc
    47761 gtaaagtagc gcagttcacc ggcacgcgct ttcttatata agccttttgg atcgcgggct
    47821 tcgcaaatcg ccagcggcgt atcgacaaac acttcgataa agcgcccttc tcctacgcgt
    47881 tcacgaacca tctggcgttc ggcgcggtgt ggcgagataa atgcggtcag caccaccagt
    47941 ccggcttcaa ccatcaaatt cgccacttca ccgacgcgac ggatattctc tttacgatcg
    48001 gcatcgctaa aaccgagatc gctgcataat ccgtggcgaa cattatcgcc atccagcaga
    48061 tacgtactga cgccgagttt atgtaacgcc tcctccagcg ccccggcgac cgttgattta
    48121 ccggacccgg agaggccggt aaaccacagc actacaccac gatgaccgtg gtgtagctcg
    48181 cgttgttgca cagtgaccgg atggctatgc cagacgacgt tttcgtcatg cagcgccatt
    48241 atttatcccc cagcaaatcg cgcgcgcccc agtgtggaaa gtggcggcga accagggcat
    48301 tcaattccag ttcgaatgca ctaaattcag atggcgcagc agttgcctgg ctaactggct
    48361 catgcaccat accggcacct acggtcacat tgctcaggcg atcgataaaa atcagcccac
    48421 cggtaaccgg gttttgctga taacgatcta acaccagtgg ctcgtcaaaa gtgagatcca
    48481 ccaggccgat gccgttcagc ggcaggtttt caacttcgcg ttgggtaagg ttattaatat
    48541 caacctgata acgaatgcca tcaacacgag cacgcgtctt cttaccggca attttgatgt
    48601 cataactctg gcccggggaa agcggctgtt ccgccatcca taccacatcc accgacgcgc
    48661 tctgcacagc tggtaacgct tcgtctgccg ccagcagcag atcgccacgg ctgatgtcga
    48721 tctcatccgt cagcaccagg gtgatcgctt ctccggcaaa ggcttcttcg cgatcgccat
    48781 caaaagtcac gatccgggcg acgtttgatt ccacaccaga gggcagcact ttgactcgtt
    48841 gcccgacttc tacgcgaccg gatgccagcg ttccggcgta accacgaaaa tcgagattcg
    48901 ggcgattaac gtactgcacc gggaagcgca ttggctgagc atccaccact cgctggatct
    48961 ccacggtttc cagcacttcg agcagtgttg gaccgctgta ccacgccata ctttcacttt
    49021 gcgaagccac gttgtcgcct tccagtgcag agagcggcac aaagcggata tccagattac
    49081 ccggcagctg cccggcaaag gtcagataat cttcacaaat acgggtgaac gtcttttcac
    49141 tgtaatccac cagatccatt ttgttgatcg ccacgaccag atgtttgatc cccaacagtg
    49201 tggagataaa actgtgacga cgggtttgat cgagcacgcc tttacgggca tcgatcagta
    49261 agatcgccag ttcacatgtc gatgcgccag tcgccatatt gcgggtgtac tgctcgtgcc
    49321 ctggggtgtc ggcgataata aatttacgct tctcggtaga gaaatagcgg taagccacgt
    49381 caatggtgat gccctgttcg cgctcagctt gcaggccgtc caccagcagt gccagatcca
    49441 gcttttcgcc ctgggtgccg tgacgtttac tgtcgttatg cagcgatgag agctgatctt
    49501 cgtagatttg gcgggtatcg tgcagcaggc gaccaatcag ggtacttttg ccgtcatcga
    49561 cgctaccaca ggtcagaaaa cgcagcaggc ttttatgttg ttgcgcaatc atccaggctt
    49621 cgacgccgcc ttcattggcg atttgttgtg caagtgcggt gttcatctta aaaatacccc
    49681 tgacgttttt tcagctccat agaccccgcc tggtcgcggt caatcacgcg gccctgacgt
    49741 tcactggtgg tagaaaccag catctcttcg atgatctccg gtagtgtttg tgcatttgac
    49801 tccaccgcac cggtcagcgg ccagcagccc agcgtacgga aacgcaccat ccgtttttta
    49861 atcacttcgc ccggttgcag gtcgatacgg ttgtcatcaa tcatcatcaa cataccgtcg
    49921 cgttccagaa ccggacgttc cgcagcgaga tacagcggca caatgtcgat attttccagc
    49981 cagatgtatt gccagatatc ctgctcggtc cagttagaga gcgggaagac gcggatgctt
    50041 tcgcctttgt taatttgccc gttgtagttg tgccacagct ctgggcgctg atttttcgga
    50101 tcccagcgat ggaagcggtc acggaaagag tagattcgct ctttagcgcg ggatttctct
    50161 tcgtcacggc gcgcaccacc gaaggcggca tcaaaaccgt atttgttcag cgcctgtttc
    50221 aggccttcag ttttcataat atcggtatgt ttcgcgctgc cgtgcacgaa tggattaatc
    50281 cccatcgcca cgccttccgg gtttttatgc accagcagct cgcagccgta ggctttcgcc
    50341 gtacgatcgc ggaactcata catctcgcgg aatttccagc cggtatcgac atgcagcaac
    50401 gggaaaggca gcgtacctgg ataaaacgcc ttgcgcgcca gatgcagcat gacgctggaa
    50461 tctttaccga tagagtagag catcaccgga tttgagaatt ctgccgccac ctcgcgaata
    50521 atgtggatgc tttccgcctc cagttgccgc aggtgagtaa gtcgtatttg atccataacc
    50581 gttcctttgc aataccgcta ttttcttgcc atcagatgtt tcgactatag ggagcgtaag
    50641 agaacgaatg aaattaccaa ttagaatgag tagttcctta acggaataac gatttggcaa
    50701 agctaatatc aaaaagtgct taaggcaccg gatttcgggc gtttaggaag atttgaaatt
    50761 gttttagcgc agcggcagtt tcatactatg gcggtaaaaa aatttgcatg gtatttaagg
    50821 actcactatg ttttccgcat tgcgccaccg taccgctgcc ctggcgctcg gcgtatgctt
    50881 tattctcccc gtacacgcct cgtcacctaa acctggcgat tttgctaata ctcaggcacg
    50941 acatattgct actttctttc cgggacgcat gaccggaact cctgcagaaa tgttatctgc
    51001 cgattatatt cgccaacagt ttcagcaaat gggttatcgc agtgatattc ggacatttaa
    51061 tagtcggtat atttataccg cccgcgataa tcgtaagagc tggcataacg tgacgggaag
    51121 tacggtgatt gccgctcatg aaggcaaagc gccgcagcag atcatcatta tggcgcatct
    51181 ggatacttac gccccgctga gcgatgctga cgccgatgcc aatctcggcg ggctgacgtt
    51241 acaaggaatg gatgataacg ccgcaggttt aggtgtcatg ctggaattgg cagaacgcct
    51301 gaaaaatacg cctaccgagt atggtattcg atttgtagcg accagcggtg aagaggaagg
    51361 gaaattaggc gctgagaatt tactcaagcg gatgagtgac accgaaaaga aaaatacgct
    51421 gctggtgatt aatctcgata acttaattgt tggcgataaa ttgtatttca acagtggtgt
    51481 aaaaacccct gaagcagtaa ggaaattaac gcgcgacagg gcactggcaa ttgcgcgtag
    51541 tcatggaatt gccgcaacga ccaatccggg tttgaataaa aattatccga agggtactgg
    51601 ctgttgtaat gacgccgaag ttttcgacaa agccggtatt gccgtacttt cggtagaagc
    51661 aacaaattgg aatcttggga ataaggatgg ttatcagcaa cgcgcaaaaa cagctgcatt
    51721 ccctgcggga aatagctggc atgacgtaag actggataat caacaacata ttgataaggc
    51781 acttccagga agaatagaac gtcgctgccg tgatgttatg cggataatgc taccgctggt
    51841 gaaggagctg gcgaaggcgt cttgatgggt atgaaaatgg gagttgggag ttctaccgca
    51901 ggggcgggga actccaagtg atatccatca tcgcatccag tgcgtccggt ttatcctcgc
    51961 tgacgcgggg aactcttggt tgttcggcgt tttgaagaga acaaaaacgg tttatccccg
    52021 ctggcgcggg gaactcttcc cgcgcgattg cgatcgccat cgtccgatcg gtttatcccc
    52081 gctggcgcgg ggaactcatg taatgaatat taagcttttc acattgtccc ggtttatccc
    52141 cgctggcgcg gggaactctg ttgcagcggc gcaccgctga cggactcgcc cggtttatcc
    52201 ccgctggcgc ggggaactca aaaccaccgg acctgaccat ttgaaatgag ccggtttatc
    52261 cccgctggcg cggggaactc agccggatgc tgatcgatac cgcgcatcac cgcggtttat
    52321 ccccgctggc gcggggaact caattcataa attaatttac tattttgagt gcgcggttta
    52381 tccccgctgg cgcggggaac tccattaatt gggaacgcct catcactgcc accgcggttt
    52441 atccccgctg gcgcggggaa ctcagatcgg aatggctgcg gtcgtcgcat tttttcggtt
    52501 tatccccgct ggcgcgggga actcggtgaa ccccatcacc aagaacatga agccgtcggt
    52561 ttatccccgc tggcgcgggg aactctgagg tattccgcca gggcggacaa tttatcacgg
    52621 tttatccccg ctggcgcggg gaactcaagc gcgccccatt taacgccgct gccgacggcg
    52681 gtttatcccc gctggcgcgg ggaactccct tttgatattc gtgaaccaga cttttagtac
    52741 ggtttatccc cgctggcgcg gggaactctc taaaagtata catttgttct taaagcattt
//
LOCUS       DAPHBJ010000008        49501 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.008, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000008 DAPHBJ010000000
VERSION     DAPHBJ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.008"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(135..839)
                     /locus_tag="RHZ76_000452"
     CDS             complement(135..839)
                     /locus_tag="RHZ76_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDV2811154.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(976..1428)
                     /gene="moaE"
                     /locus_tag="RHZ76_000453"
     CDS             complement(976..1428)
                     /gene="moaE"
                     /locus_tag="RHZ76_000453"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HDV2811155.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVNALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDRQAAKRW"
     gene            complement(1430..1675)
                     /gene="moaD"
                     /locus_tag="RHZ76_000454"
     CDS             complement(1430..1675)
                     /gene="moaD"
                     /locus_tag="RHZ76_000454"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HDV2811156.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHLLTDGDEVAFFPPVTGG"
     gene            complement(1668..2153)
                     /gene="moaC"
                     /locus_tag="RHZ76_000455"
     CDS             complement(1668..2153)
                     /gene="moaC"
                     /locus_tag="RHZ76_000455"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDV2811157.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2156..2668)
                     /gene="moaB"
                     /locus_tag="RHZ76_000456"
     CDS             complement(2156..2668)
                     /gene="moaB"
                     /locus_tag="RHZ76_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HDV2811158.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2690..3679)
                     /gene="moaA"
                     /locus_tag="RHZ76_000457"
     CDS             complement(2690..3679)
                     /gene="moaA"
                     /locus_tag="RHZ76_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDV2811159.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDAQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3667..3811)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4076..4984
                     /gene="yvcK"
                     /locus_tag="RHZ76_000458"
     CDS             4076..4984
                     /gene="yvcK"
                     /locus_tag="RHZ76_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HDV2811160.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5176..7197)
                     /gene="uvrB"
                     /locus_tag="RHZ76_000459"
     CDS             complement(5176..7197)
                     /gene="uvrB"
                     /locus_tag="RHZ76_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HDV2811161.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7776..8453)
                     /gene="bioD"
                     /locus_tag="RHZ76_000460"
     CDS             complement(7776..8453)
                     /gene="bioD"
                     /locus_tag="RHZ76_000460"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDV2811162.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(8446..9201)
                     /gene="bioC"
                     /locus_tag="RHZ76_000461"
     CDS             complement(8446..9201)
                     /gene="bioC"
                     /locus_tag="RHZ76_000461"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HDV2811163.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPKGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9188..10342)
                     /gene="bioF"
                     /locus_tag="RHZ76_000462"
     CDS             complement(9188..10342)
                     /gene="bioF"
                     /locus_tag="RHZ76_000462"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HDV2811164.1"
                     /translation="MSWQDKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQSAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQMVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTHADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10339..11379)
                     /gene="bioB"
                     /locus_tag="RHZ76_000463"
     CDS             complement(10339..11379)
                     /gene="bioB"
                     /locus_tag="RHZ76_000463"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDV2811165.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            11466..12755
                     /gene="bioA"
                     /locus_tag="RHZ76_000464"
     CDS             11466..12755
                     /gene="bioA"
                     /locus_tag="RHZ76_000464"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDV2811166.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12814..13290
                     /locus_tag="RHZ76_000465"
     CDS             12814..13290
                     /locus_tag="RHZ76_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HDV2811167.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            13442..14725
                     /locus_tag="RHZ76_000466"
     CDS             13442..14725
                     /locus_tag="RHZ76_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HDV2811168.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(14959..17220)
                     /locus_tag="RHZ76_000467"
     CDS             complement(14959..17220)
                     /locus_tag="RHZ76_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HDV2811169.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(17403..18833)
                     /locus_tag="RHZ76_000468"
     CDS             complement(17403..18833)
                     /locus_tag="RHZ76_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDV2811170.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLTAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLGIVTWEDVVKNKGGWNTLIWYGGIIG
                     LSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAMLP
                     VFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGAV
                     LTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(18909..19961)
                     /locus_tag="RHZ76_000469"
     CDS             complement(18909..19961)
                     /locus_tag="RHZ76_000469"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HDV2811171.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            20145..21098
                     /locus_tag="RHZ76_000470"
     CDS             20145..21098
                     /locus_tag="RHZ76_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2811172.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(21139..22134)
                     /gene="pgl"
                     /locus_tag="RHZ76_000471"
     CDS             complement(21139..22134)
                     /gene="pgl"
                     /locus_tag="RHZ76_000471"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HDV2811173.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            22289..23107
                     /gene="ybhA"
                     /locus_tag="RHZ76_000472"
     CDS             22289..23107
                     /gene="ybhA"
                     /locus_tag="RHZ76_000472"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HDV2811174.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVIKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(23108..24166)
                     /gene="modC"
                     /locus_tag="RHZ76_000473"
     CDS             complement(23108..24166)
                     /gene="modC"
                     /locus_tag="RHZ76_000473"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HDV2811175.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(24169..24858)
                     /gene="modB"
                     /locus_tag="RHZ76_000474"
     CDS             complement(24169..24858)
                     /gene="modB"
                     /locus_tag="RHZ76_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDV2811176.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(24858..25631)
                     /gene="modA"
                     /locus_tag="RHZ76_000475"
     CDS             complement(24858..25631)
                     /gene="modA"
                     /locus_tag="RHZ76_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV2811177.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQFKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASVQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTIK"
     gene            complement(25798..25947)
                     /gene="acrZ"
                     /locus_tag="RHZ76_000476"
     CDS             complement(25798..25947)
                     /gene="acrZ"
                     /locus_tag="RHZ76_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HDV2811178.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            26076..26864
                     /gene="modE"
                     /locus_tag="RHZ76_000477"
     CDS             26076..26864
                     /gene="modE"
                     /locus_tag="RHZ76_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HDV2811179.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            26932..28404
                     /gene="modF"
                     /locus_tag="RHZ76_000478"
     CDS             26932..28404
                     /gene="modF"
                     /locus_tag="RHZ76_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HDV2811180.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            28666..29682
                     /gene="galE"
                     /locus_tag="RHZ76_000479"
     CDS             28666..29682
                     /gene="galE"
                     /locus_tag="RHZ76_000479"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDV2811181.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            29692..30738
                     /gene="galT"
                     /locus_tag="RHZ76_000480"
     CDS             29692..30738
                     /gene="galT"
                     /locus_tag="RHZ76_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDV2811182.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESHDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            30742..31890
                     /gene="galK"
                     /locus_tag="RHZ76_000481"
     CDS             30742..31890
                     /gene="galK"
                     /locus_tag="RHZ76_000481"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDV2811183.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            31884..32924
                     /gene="galM"
                     /locus_tag="RHZ76_000482"
     CDS             31884..32924
                     /gene="galM"
                     /locus_tag="RHZ76_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HDV2811184.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            33127..33879
                     /gene="gpmA"
                     /locus_tag="RHZ76_000483"
     CDS             33127..33879
                     /gene="gpmA"
                     /locus_tag="RHZ76_000483"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDV2811185.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(34037..35089)
                     /gene="aroG"
                     /locus_tag="RHZ76_000484"
     CDS             complement(34037..35089)
                     /gene="aroG"
                     /locus_tag="RHZ76_000484"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HDV2811186.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            35405..35785
                     /locus_tag="RHZ76_000485"
     CDS             35405..35785
                     /locus_tag="RHZ76_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HDV2811187.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            35899..36840
                     /gene="zitB"
                     /locus_tag="RHZ76_000486"
     CDS             35899..36840
                     /gene="zitB"
                     /locus_tag="RHZ76_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HDV2811188.1"
                     /translation="MAHSHSNTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(36837..37556)
                     /gene="pnuC"
                     /locus_tag="RHZ76_000487"
     CDS             complement(36837..37556)
                     /gene="pnuC"
                     /locus_tag="RHZ76_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HDV2811189.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(37594..38637)
                     /gene="nadA"
                     /locus_tag="RHZ76_000488"
     CDS             complement(37594..38637)
                     /gene="nadA"
                     /locus_tag="RHZ76_000488"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDV2811190.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVEERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(38915..38990)
                     /locus_tag="RHZ76_000489"
     tRNA            complement(38915..38990)
                     /locus_tag="RHZ76_000489"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38955..38957),aa:Lys,seq:ttt)
     gene            complement(39123..39198)
                     /locus_tag="RHZ76_000490"
     tRNA            complement(39123..39198)
                     /locus_tag="RHZ76_000490"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39163..39165),aa:Lys,seq:ttt)
     gene            complement(39345..39420)
                     /locus_tag="RHZ76_000491"
     tRNA            complement(39345..39420)
                     /locus_tag="RHZ76_000491"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39385..39387),aa:Lys,seq:ttt)
     gene            complement(39424..39499)
                     /locus_tag="RHZ76_000492"
     tRNA            complement(39424..39499)
                     /locus_tag="RHZ76_000492"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39464..39466),aa:Val,seq:tac)
     gene            complement(39551..39626)
                     /locus_tag="RHZ76_000493"
     tRNA            complement(39551..39626)
                     /locus_tag="RHZ76_000493"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39591..39593),aa:Lys,seq:ttt)
     gene            complement(39629..39704)
                     /locus_tag="RHZ76_000494"
     tRNA            complement(39629..39704)
                     /locus_tag="RHZ76_000494"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39669..39671),aa:Val,seq:tac)
     gene            complement(39840..39915)
                     /locus_tag="RHZ76_000495"
     tRNA            complement(39840..39915)
                     /locus_tag="RHZ76_000495"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39880..39882),aa:Lys,seq:ttt)
     gene            complement(40080..40871)
                     /gene="cpoB"
                     /locus_tag="RHZ76_000496"
     CDS             complement(40080..40871)
                     /gene="cpoB"
                     /locus_tag="RHZ76_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HDV2811191.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(40881..41402)
                     /gene="pal"
                     /locus_tag="RHZ76_000497"
     CDS             complement(40881..41402)
                     /gene="pal"
                     /locus_tag="RHZ76_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HDV2811192.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(41437..42729)
                     /gene="tolB"
                     /locus_tag="RHZ76_000498"
     CDS             complement(41437..42729)
                     /gene="tolB"
                     /locus_tag="RHZ76_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HDV2811193.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(42862..44127)
                     /gene="tolA"
                     /locus_tag="RHZ76_000499"
     CDS             complement(42862..44127)
                     /gene="tolA"
                     /locus_tag="RHZ76_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDV2811194.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(44192..44620)
                     /gene="tolR"
                     /locus_tag="RHZ76_000500"
     CDS             complement(44192..44620)
                     /gene="tolR"
                     /locus_tag="RHZ76_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HDV2811195.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(44624..45316)
                     /gene="tolQ"
                     /locus_tag="RHZ76_000501"
     CDS             complement(44624..45316)
                     /gene="tolQ"
                     /locus_tag="RHZ76_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HDV2811196.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(45313..45717)
                     /gene="ybgC"
                     /locus_tag="RHZ76_000502"
     CDS             complement(45313..45717)
                     /gene="ybgC"
                     /locus_tag="RHZ76_000502"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDV2811197.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(45867..46160)
                     /gene="ybgE"
                     /locus_tag="RHZ76_000503"
     CDS             complement(45867..46160)
                     /gene="ybgE"
                     /locus_tag="RHZ76_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HDV2811198.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(46160..46273)
                     /gene="cydX"
                     /locus_tag="RHZ76_000504"
     CDS             complement(46160..46273)
                     /gene="cydX"
                     /locus_tag="RHZ76_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HDV2811199.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(46288..47427)
                     /gene="cydB"
                     /locus_tag="RHZ76_000505"
     CDS             complement(46288..47427)
                     /gene="cydB"
                     /locus_tag="RHZ76_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDV2811200.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREVGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(47443..49011)
                     /gene="cydA"
                     /locus_tag="RHZ76_000506"
     CDS             complement(47443..49011)
                     /gene="cydA"
                     /locus_tag="RHZ76_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDV2811201.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT        12139 a        13181 c        12274 g        11907 t
ORIGIN      
        1 ttttgtattt atggatacgg aaaacgcttc cgctcgtcca taaggcgtat tttatacctg
       61 gtacttgtta agtgtcgttt ttagtaagaa aaaaaatccc gacacatggc cgggatattg
      121 atggtgaaaa agaattaacg gcggttgccg aagatccgca gcaacatcag gaacaggttg
      181 atgaagtcca gatacaaggt taacgcgcca agaatggaat atttgcgcag gttcgacgtg
      241 tcgcgggtat caatctgctc acccatattt ttcagtttct gggtgtcata cgccgtcaat
      301 ccgacaaaga caatcacgcc gatgtaggtg actgcccaca tcaacgcttc gcttttcagc
      361 cagaagttga ccagcgatgc cagcacaatg ccgattaacg ccataaacag catattgcca
      421 aagccgctta gatcgcgctt cgtggtgtaa ccatacaggc tcattgcgcc gaacatcccg
      481 gcagtaacga cgaaagtact ggcgatagaa gcagcggtat agacaatgaa aatactggaa
      541 agcgtaagac ccgtcagcgc cgaataaagc ataaagagca tagtcgttac acctgcgctc
      601 agcttttgaa tcatcgctga taacacaata accaatgcta attgcgcgat gatcagaccg
      661 attaaaaaga cacggttagt gaacaacagc tccatcactg ccgcagaatt agctgcatac
      721 caggcaacaa atgcggtcag caacaagcca acggtcatcc agccatagac ttgcgccata
      781 taagtctgca agccagcccg gggttgtaca attgaatcag aacgtgggaa tctgtccatg
      841 acgatctcct gaagatatta ggaatatctt aaggatactg caaaatgatg aggctgtgca
      901 tcgacgcagc gtaaacgcat gtactgagcg gtgaaattgc cggacgcagc ggtgccttat
      961 ccggctaaca aaaaactacc agcgttttgc cgcctgccga tcgctctccc gagcttcgac
     1021 ccagcggtcg ccttccggcg tggcttcacg cttccagaac ggtgcgcggg ttttgagata
     1081 atccataata aactgcccgg cttcaaacgc actgctgcga tgcgcactgg tgacaccgac
     1141 aaaaacgatt tcatcgcccg gccataattc cccgatgcgg tgaatcacag tgacgcgccc
     1201 cagcggccag cggttacgcg cttcatcaac aatttctgcc agtgcttttt cagtcatccc
     1261 cggatagtgt tcgagggtta atgcgttgac gctgtcgccc aggttatggt tgcgcacctt
     1321 accagtaaag gtgactaccg caccgtcttc gtcacgctcc gccagccacg ggtactcttc
     1381 tcctacgctg aacggctgcg gaccaacaac aattttggtt tctgccatct taacctccgg
     1441 ttaccggcgg gaagaaagct acttcgtcgc cgtcagtcag cagatggtca aaactcacca
     1501 gcgtctggtt gacggcagcc agtaatttgc catcttccag cgccagcgcc cagcgatcgc
     1561 tctgcgcagc catgtgctgg cgtaacgctt caacagttgg gaaatccgca gccacttcgg
     1621 ttgcatctgt tcccaccaac tcgcgcacct gggcgaaaaa aagaacttta atcatccgct
     1681 tccaccttaa agtcacccga cttgccgccg ctcttcgcca gcaaacgtac cggaccaatc
     1741 accatatctt tttgcaccgc tttgcacatg tcataaatgg tcagcgccgc cacggaggcc
     1801 gcggttaatg cttccatttc gacaccggtt ttcccggtca ggcggcataa ggtttctata
     1861 cgcacccgat tgtgctccgg ctcggcctgt aaattgactt caactttgct gagcatcagc
     1921 ggatgacaga gcgggatcag atcccaggtg cgttttgccg cctgaatacc ggcaatacgc
     1981 gcagtggcaa atacgtcgcc tttgtggtgg cgaccatcaa taatcatcgc cagcgtctcg
     2041 ctgcgcatgg tgacaaaggc ttccgcccgc gcttcacgca cggtttccgc tttggcggag
     2101 acatccacca tgtgcgcttc gccagcggcg ttgatatggg tcagttgcga catacttatt
     2161 tcttcaaatg tggatggaaa ttacacggac gcgtacgggc atccaactgc ggcgcgatga
     2221 tattttccca tgcggtacgg cacgctttgg tcgaacccgg catggcgaaa atcagcgttt
     2281 tgttagcgac gcccgctacc gcacgagatt gcaacgtgga agtgccaatc tcttcaaacg
     2341 acaacatacg gaacacttca ccaaaacctt caacttcacg gtcgaacaac ggcagcaatg
     2401 cttcgggagc ctgatcacct tccgtcaggc cagtaccacc cgtaatcaac accacttgta
     2461 catcgtcgct ggcgatccac gcagatacct gagcgcgaat agcgtagcgg ttttctttca
     2521 caatggcttt atcgacaacg tgatggcccg cttcttgcgc cgaatcgcgc aaatagtgac
     2581 cggaggtatc gtcttcttca ccgcgccgat tagaaaccgt aagaatagca atacgggtcg
     2641 ggataaattc agtgcttacc tgactcatct gatctctcct tttgacgttt tagccgccaa
     2701 tgtacgataa gttttgcgta ataccggtgt tgttttgatg caggaaatgg gtctgtttct
     2761 tctcccgcag cgccgctgaa atacgcgctt ccagcgcctg ttgctgggca tcgtcttcca
     2821 gcagatcgcg caggttaacg ccgccttcac caaacaggca gagatggagt ttaccaatgg
     2881 aggaaacgcg caggcggttg caagtggcgc agaagtcttt ttcatacggc atgataaggc
     2941 caatctctcc ggcgtaatcc ggatggcaaa agacttgcgc gggaccgtcg ctgcgttgac
     3001 gtaattggtg gatccagccg cgacgcagta gctcgtcacg cagaacctga ccagagatgt
     3061 gatgcttacg gaagagctca ctgccctcgc ccgtttccat cagttcgatg aaacgcagct
     3121 ggatagggcg atgctggatc cagttcagaa aggtgtcgag ctggtgatga ttaacatcac
     3181 gcatcagcac ggtattgact ttgaccttct caaaaccggc ctcaaatgca gcatcaatcc
     3241 ctgccatgac ctggttgaat ttatcctgcc cggtaatagc gtgaaactgg cgggcgtcca
     3301 gactgtcgac actgacgtta atgccagtaa gtcccgcatc gcgccagttc gccacatcgc
     3361 gttccagacg gtaaccattg gtggtgaccg caatctggcg gatagcgtcg ttttcccgca
     3421 cagcggcgat gatatcggta aagtcgcggc gtaaagacgg ctctcctcct gtcaggcgca
     3481 ctttttcggt gcccagactg gcgaaggcgc gcgtaacccg gcgaatttca tcgacggtaa
     3541 gaaagccttt attggtgacg ccgctcggtt tgtagccatc cggcaggcag taggtgcaac
     3601 gaaagttaca cacatcggta atcgacaggc gcaagtagta aaacttacgc gcaaatgcat
     3661 cagtcagttg tgaagccatg tacacctttc cagatacggg aggcgaagtc atttcttcct
     3721 tcgccctggt ggcaatcttt acgagcattg tcacggccaa agcaccgtat cagttgaccc
     3781 aggtgcagag gctagagtgt ttagtggtta agccgatact agcgtgtaaa tgagattttt
     3841 accatccaca ctttcgctat gtaatcatgt atatagcgtc atgatcgtgc tatttcgcta
     3901 cagaataagg gaaaatggca gttttacttt gatatacatc atcagggcgg catagcgtgc
     3961 atcgtccttt agaggtagaa gaaaatagtt tgtatcctca atattggcag gttaattgct
     4021 gtttccccgc aatttgcgct actgtagcgc ggataatctg actccaggaa actatatgcg
     4081 caatcgtacg ctggctgatc ttgatcgtgt cgttgctctc ggcggagggc atggactggg
     4141 acgcgttctc tcatcacttt cgtctttggg ttctcgttta acgggtatcg tcaccaccac
     4201 cgataatggt ggctcgacgg ggcgtattcg ccgttcagaa ggcggcattg cctggggcga
     4261 tatgcgcaac tgcctcaatc agctgataac ggaaccgagc gtcgcctccg cgatgtttga
     4321 ataccgtttt ggtggcaatg gcgaactttc cggtcataat ctcggaaact tgatgttaaa
     4381 ggcgctggat caccttagcg tgcggcctct ggaagccatc aatttaattc gtaatctgct
     4441 gaaagtggat acgcatttga ttccaatgtc agagcatcct gttgatctga tggcgattga
     4501 tgatcagggg catgaagttt acggcgaggt caatatcgac cagttaacta cgccgattca
     4561 agagttattg ttaacgccta atgtacccgc aacgcgtgag gcggttcacg ctatcaatga
     4621 agcggatctc atcattattg ggcctggcag tttttatacc agcctgatgc caattctgct
     4681 gctgaaggaa atcgcccagg cattacgccg cacgccagcg ccgatggttt atatcggcaa
     4741 tctggggcgt gagttgagtt tacctgcggc taatttgaag ctggaaagca agctggcaat
     4801 tatggagcag tatgttggta aaaaggtcat tgatgcggtc atcgtcgggc caaaagtgga
     4861 tgtctcggcg gtgaaagagc ggattgtgat ccaggaggta ctggaggcca gcgatattcc
     4921 gtatcgtcat gaccgccagt tgttacataa cgcgctggaa aaggcgttac aggctttagg
     4981 ttaacggaaa tccgaaggaa aattccggct tcctattgaa gacaaagtgc gcgttgttta
     5041 tgccggatgc ggcgtgaacg ccttatccgg cctacaaacc gcgctaattc aatgtattgc
     5101 agagatcatg taggcctgat aagcgtagca catcaggcaa ttttgctttt gtcatctgtc
     5161 ttcatcgtta tcctgtcacg acgccgcgat aaacaattca cgcagctgat gcaactggtc
     5221 acgaatttgc gccgcttctt cgaactccag attctgcgcg tgttgcatca tcaacccttc
     5281 cagctcatgg attttctgct gcaacgcttt aggcgacata tccatcggca cattatccgg
     5341 ctcaacaatc gggcgcgatt ttcctctgcc cttcgctttg gttttggcaa tgttctgccc
     5401 cagcgccagg atatcgacca ctttcttgtt caagccttgc ggcgtaattc cgtgttcctc
     5461 gttgtacttc tgctgtttct cgcggcgacg ttcggtttcg ccaatcgctt tcgccattga
     5521 tggggtgatc ttatcgccgt agagaatcgc tttaccgtta acgttacgtg ccgcacgacc
     5581 aatggtctgg atcaacgaac gttcggaacg caggaagcct tctttgtcag cgtcgaggat
     5641 cgccacaagc gacacttccg gcatatccag accttcgcgc agtaagttga tccctaccag
     5701 cacgtcgaac tcacccagac gcaagtcgcg gataatctcc atacgttcga cggtgtcgat
     5761 atctgagtga agataacgca cgcgctcgcc atgttcttcg agatattcgg taagatcttc
     5821 cgccatccgc ttggtcagtg tggtgaccag tacgcgttcg ttaattgccg ctcgctgacg
     5881 aatctccgaa agaagatcat caacctgtgt cgccaccggc cgcacttcga taatcgggtc
     5941 aagcaatccg gttggacgca ccacctgatc caccacatcg ccgccggatt tttccagctc
     6001 gtaattaccc ggcgtcgccg aaacatagat ggtttgcggc gctaatgctt cgaactcttc
     6061 aaacttaagc ggacggttat ccagcgctga tggcaggcgg aagccgtact ccaccagtgt
     6121 ctctttacgc gcccggtcac cgcgatacat gccgccaatt tgtggaatgg tgacgtgaga
     6181 ttcatcgacc accagcagcc catcagcagg gaggtaatca aacagcgtcg gcggtggctc
     6241 acccggtcca cgaccggaga ggaagcgcga gtagttttca atccccgaac agtagcccag
     6301 ctcgttcatc atctccagat caaactgggt acgctgggtc agccgctgct cttccagcag
     6361 tttgttgttt tccaacagca ctttgcgtct ggcggccagc tcttctttga tctcctccat
     6421 cgcctgtacg atgcgctcgc gcggtgtgac gtagtgcgtt ttcgggtaga tggtaaaacg
     6481 tggaatagtg gaaacaatct gcccggtcag cgggtcaaat aacgacaatc gttccacttc
     6541 ctcgtcaaac agttccacgc gaagtgcaat gtcatccgat tctgccggga agatatctat
     6601 cacctcgcca cgaacgcgga aagtaccacg ctggaatgct tgatcattac gagcgtattg
     6661 cagctccgcc agtcggcgca gaatcgcgcg ctgatcgata atcataccga ccgtgagatg
     6721 gagcatcatc ttgagatata aatcaggatc gcccagacca taaatcgcgg aaacagacgc
     6781 caccacaacc acatcacgcc gctccagcat cgctttggtg gcggataaac gcatctgctc
     6841 aatatgttcg ttaaccgagg catctttctc aatgaaagtg tcggaactcg gtacataggc
     6901 ttccggctga tagtagtcgt agtaggagac gaaatactcc accgcgtttt ccgggaagaa
     6961 ctctttcatt tcgccataca gctgggccgc cagcgttttg ttgggcgcaa gtaccatggt
     7021 tgggcgctga aggtcagcaa tgacattggc aatggtgaag gttttccctg agccagtcac
     7081 gccaagtaac gtctggtgcg ccaggccatc ttccagcccc tcttcgagac gtcgaatcgc
     7141 ctctggctga tcgccagaag gtttaaaagc ggaattcagt ttgaacggtt tactcatgag
     7201 tcgctacctg aaggagttgg gcgggcaggt atgtaatttt actcgtcgta cttaattatg
     7261 ccaacaaatt atactggata aaaaaacagt tcatcaccat aatatttctg atacagcgta
     7321 aactccgctg tcaatcatga gcaaaattta ctctgtggcg agataaaact ccggccttac
     7381 cgggttatcc ccaaagcaac ggctttttta acatttgtca agatgagtga tgacagtttt
     7441 atggcaagag atgcctgttc agtgactgcc attgcattta tctaaccagt taaaaaataa
     7501 aagatatttc tttgagccgt ctttaacgcc aattcgcgtg caagccgcgt attctctcgc
     7561 ttgcctcgtg ttttctaact ctaatacaca tggttatcca caggaatagt ggataactgt
     7621 ctccagcccc tatcccccgc cgcctgggca attcccacat tcgccgcatg aggcggaagt
     7681 aaaatttttt tcgtgattat tttagaattt aattggttaa attgcagtca atcgaagacg
     7741 cgatctcgct cgcaatttaa ccaaatacag aatggctaca acaaggcaag gtttatgtac
     7801 tttccggttg ccgcattttc tggattttct gcaagccagg ggatctctcc cagcagcggc
     7861 gcgggaatca tgcgggtgag cgtggtcata tattcagcgt gacgttttcc cggaggcgta
     7921 acatcgttcg ccacccaacc cgccagagtc agtccggcgt gttgtattgc ctgcgcagtc
     7981 aacatcgcgt gattaataca gccgagtttc acaccaacta ccagtatcac cggcagttgt
     8041 tcctgtgtta cccaatctgc aaaagtgaaa gtgtcagaaa gcggcgtaaa ccagccgcca
     8101 gcaccttcca ctaacaccca gtcagcctgt tgttcaagcg cgcgtaatcc ggcgctcatt
     8161 accaatgatt ctatcggtct gccctcttgc gcgctgatga tgtgcggcga agtgggttct
     8221 gcgaaggtgt aaggatttac tgttgcgtaa tccagctgca ggctgctgtt gcgctgtaac
     8281 gccagcgcgt cgctattgcg taaaccttcc ggggtctttt cgctgccaga ggcgaccggt
     8341 ttataacctg ccgtccggta gcctgctgcc tttgcggctt gtaaaagtgc acaactggcg
     8401 acagttttcc ccacttcggt atccgttccg gtgacaaaat aacgtttact cacgagcaat
     8461 cactcccaaa aaaagatgat acgtcagagg atatcgcccc tgctgttgcg gccaggccag
     8521 ttgcaatcgc tgcaactgcg aacgcgttaa tattcgcggg tcgcgccctt catgaagatg
     8581 cgtggcaccg atgcctttca gcgaacgcat ggcactgagc gcatcatcaa accacagcgt
     8641 gatgggctga atatgatgtt gataatgcac gccgttcagc gactgttcga tttcatctgg
     8701 cggtaaaaag cgattagcat gcggacgctc gtccaccgcc tgccacgcct gatgcagttc
     8761 gggtaacgat ccctgcacca gcgtggtaaa cgcgaccacg cctttggggc gcaccacccg
     8821 atacagctcg cggagtgccg tggataaatt accgcaccac tgcactgcga gattgctcca
     8881 tgcaagatcg aacgtcgcag tcgctaacgg cagggattcg atatctcccg ccagataatg
     8941 gtctgcggca tccttctggc gtgcctgaac aagcattggc ggcgagagat ctaaggccgt
     9001 cacctgcgcg tgacgttccc gccagtggcg gctcatccag ccaggtccac aacccgcgtc
     9061 cagtacgtgg gtgtatttac gctgtggaag cattgccagt aaggcgtcag cactctggcg
     9121 ctgtagatct gcatgttgct catagtgtgc ggctgcccga ccaaatgccg ctgcaatggc
     9181 ttgtttatta accgttgcca tgcagcacct ccagcagacg gtcgatatcc tgcatttcat
     9241 gcgcagcggt tagcgttaag cgcagtcgcg cagtaccagc gggtacggtt ggcgggcgaa
     9301 tcgccgtgac ccagcagcct tgctggcgca gtttttctgc cagttgtaac gcacggctgt
     9361 tatcaccgac aatcaatggc tggatggcgc tgcatgaatc agcatgcgta aacggcaaat
     9421 cctgtactcc ggcacgaaaa cgcgtaatga gtgccgccag tttttcgcgc cgtgcatcac
     9481 cctcatcact gcgaatgacc gccagcgacg cacgtaatgc ctgcgcctga gcgggcggca
     9541 tactggtgct gtagataagg tggcgggcga attgcagcag ataatccgcc accgtactgg
     9601 agcaaagcac cgctgccccg ctgacgccaa atcctttgcc aaaagtcact accagcaatt
     9661 ctggttttac cttttgcagc cagcagctgc cgcgcccctg ctccccgata acgcccgtgc
     9721 cgtgggcatc atcgaccatc aaccagccat tgtgctgttg cgttacctgc tggatttccg
     9781 ccagtggcgc actatcgccg tccatgctga acacgccttc tgtcaccacc atttgctgcc
     9841 ccggacaggg ggaagcaagc agtcgcgcca gatgagtgac atcgttatga acaaaacggc
     9901 gaagctgcga cgggcttaaa ctggcagcct ccagcaatga ggcatggcta agccggtcgg
     9961 caacaatacg gtcctctttc gccatcatcg cggcaatgac cgcctgatta gcggcaaaac
    10021 cagagataaa cagcagtgcc cgcgaatagc ccagccactc ggccagttct tcttccagcg
    10081 cctgatgtgc cacgctataa ccgctgacgt gaccggagcc gccgctaccg acgccaaatt
    10141 gctccgcact ctgcttccag gcacggataa tttgcggatg atggcttaaa cctaaataat
    10201 cgttactgga aaagttcaga tactggcgat cgtccgccac cagccagcgt cctgctcctt
    10261 gcgccactgg ataacggcga cgcagggcat cggcagcacg ccgcgcatcg agggccgcgt
    10321 tgattttatc ctgccagctc atagtgctgc cgcgttgtaa tattcgtcag tgtccggggt
    10381 catcagtgcc tgttccagac gctgctgttg ttcgttatcg cccgccagca cggcagtttg
    10441 ctgcggattt agccccagtt tgcggaacag ttgcaggtct ttatcttctt ccggattcgg
    10501 cgtggtcagc agtttgcaac cgtagaaaat cgagtttgcg cctgccataa agcacatcgc
    10561 ctgagtctgt tcgttcatct gctcgcgtcc ggcagaaagg cgcacgtaag aggttggcat
    10621 catgatccgc gcgaccgcaa tggtgcgaat aaaatcaaag gcatcgacat catcgttatc
    10681 ggcaagcggc gtgcctttca ccttcaccag catgttgatt ggcacgcttt ccggcggcgt
    10741 cggcaggttt gccagttgca gcaataatcc ggcgcgatct tttaccgttt cgcctaagcc
    10801 cacaatgccg ccagaacaga ctttgatccc ggcatcgcgc actttttcca gcgtatcgag
    10861 gcgttcctga taagtgcgtg tggtgatgat attgccgtaa aactccggcg aggtgtccag
    10921 gttgtggttg tagtaatcca gcccggcgtt cgcgaggcgc tgcgcctgag attcactcaa
    10981 cgtgcccagc gtcatacacg cctccagccc catcgctttt accccctgca ccatttgttc
    11041 caggtacggc atatcgcgtt cgtggggatt cttccacgcc gcgcccatac agaagcgcgt
    11101 cgatcctgcc gctttcgctt tgcgcgccga ctccagcacc tgttcaactt ccatcaaccg
    11161 ctcggcttcc agcccggttt tgtagcgcga gctttgcggg cagtatttgc aatcttccgg
    11221 acaagctccg gtcttaatcg acagcaaagt gctgacctgc acctgacgag gatcgaaatg
    11281 ctgtcgatgt acctgctgcg cttcaaacag cagatccagc aacggttttt caaataattc
    11341 tgtgacttgc gacaatgtcc agcgtgggcg gtgagccatg gggcttctcc aaaacgtgtt
    11401 ttttgttgtt aattcggtgt agacttgtaa acctaaatct tttcaatttg gtttacaagt
    11461 cgattatgac aacggacgat cttgcctttg accaacgcca tatctggcac ccatacacat
    11521 ccatgacctc ccctctgccg gtttatccgg tcgcgagcgc cgaaggttgc gagctaattt
    11581 tgtctgacgg cagacgcctg gttgacggta tgtcgtcctg gtgggcggcg atccacggct
    11641 ataatcaccc gcagcttaat gcggcgatga agtcgcaaat tgatgctatg tcgcatgtga
    11701 tgtttggcgg tatcacccat gcgcccgcca tcgagctatg ccgcaaactg gtggcaatga
    11761 cgccgcaacc gctggaatgc gtttttctcg cggactccgg ctccgtagcg gtggaagtgg
    11821 cgatgaaaat ggcgttgcag tactggcagg ccaaaggcga agcgcgccag cgttttctga
    11881 ccttccgcaa tggttatcat ggcgatacct ttggcgcgat gtcggtgtgc gatccggaca
    11941 actcaatgca cagcctgtgg aaaggctatc tgccagaaaa cttgtttgct cccgccccac
    12001 aaagccgtat ggatggcgaa tgggatgagc gcgatatggt gggctttgcc cgcctgatgg
    12061 cggcgcatcg tcatgaaatc gcggcggtga tcattgagcc gattgtccag ggcgcaggcg
    12121 ggatgcgcat gtaccatccg gaatggttaa aacgaatccg caaaatatgc gatcgcgaag
    12181 gtatcttgct gattgccgac gagatcgcca ctggatttgg tcgtaccggg aaactgtttg
    12241 cctgtgaaca tgcagaaatc gcgccggaca ttttgtgcct cggtaaagcc ttaaccggcg
    12301 gcacaatgac cctttccgcc acactcacca cgcgcgaggt tgcagaaacc atcagtaacg
    12361 gtgaagccgg ttgctttatg catgggccaa cttttatggg caatccgctg gcctgcgcgg
    12421 cagcaaacgc cagcctggcg attctcgaat ctggcgactg gcagcaacag gtggcggata
    12481 ttgaagtaca gctgcgcgag caacttgccc ccgcccgtga tgccgaaatg gttgccgatg
    12541 tgcgcgtact gggggccatt ggcgtggtcg aaaccactca tccggtgaat atggcggcgc
    12601 tgcaaaaatt ctttgtcgaa cagggtgtct ggatccggcc ttttggcaaa ctgatttacc
    12661 tgatgccgcc ctatattatt ctcccgcaac agttgcagcg tctgaccgca gcggttaacc
    12721 gcgcggtaca ggatgaaaca tttttttgcc aataacgaga agtccgcgtg agggtttctg
    12781 gctacacttt ctgcaaacaa gaaaggaggg ttcatgaaac tcatcagtaa cgatctgcgc
    12841 gatggcgata aattgccgca tcgtcatgtc tttaacggca tgggttacga tggcgataat
    12901 atttcaccgc atctggcgtg ggatgatgtt cctgcgggaa cgaaaagttt tgttgtcacc
    12961 tgctacgacc cggatgcgcc aaccggctcc ggctggtggc actgggtagt tgttaactta
    13021 cccgctgata cccgcgtatt accgcaaggg tttggctctg gtctggtagc aatgccagac
    13081 ggcgttttgc agacgcgtac cgactttggt aaaaccgggt acgatggcgc agcaccgccg
    13141 aaaggcgaaa ctcatcgcta catttttacc gttcacgcgc tggatataga acgtattgat
    13201 gtcgatgaag gtgccagcgg cgcgatggtc gggtttaacg ttcatttcca ctctctggca
    13261 agcgcctcga ttactgcgat gtttagttaa tcactctgcc agatggcgca atgccatctg
    13321 gtatcactta aaggtattaa aaacaacttt ttgtcttttt accttcccgt ttcgctcaag
    13381 ttagtataaa aaagcaggct tcaacggatt catttttcta tttcatagcc cggagcaacc
    13441 tgtgaacaca ttttcagttt cccgtctggc gctggcattg gcttttggcg tgacgctgac
    13501 cgcctgtagc tcaaccccgc ccgatcaacg tccttctgat caaaccgcgc ctggtacctc
    13561 ttctcgcccg attctgtcgg caaaagaagc gcagaatttc gatgctcaac actattttgc
    13621 atccctgaca ccaggtgctg cagcgtggaa tccttccccg attaccctgc ctgcgcaacc
    13681 tgactttgtt gtcggcccgg cgggcactca aggtgtaacg cataccacga ttcaggcggc
    13741 ggtagatgcg gcaattatca agcgtaccaa caagcgccag tatattgccg tgatgcctgg
    13801 tgagtatcag ggaacggtat atgtccctgc cgctccgggt ggaattactc tgtacggtac
    13861 aggtgaaaaa ccgattgatg tgaagattgg gctttccctt gatggtggca tgagccctgc
    13921 cgactggcgt cacgacgtca acccgcgcgg caaatatatg ccaggtaaac cagcgtggta
    13981 tatgtacgat agctgccaga gcaaacgcag cgacagtatc ggtgttctct gctctgcggt
    14041 cttctggtca caaaacaatg gcctgcaact gcaaaatctg accatcgaaa acacgctggg
    14101 cgatagcgta gatgcaggta accatccggc ggtggcactg cgtactgatg gtgaccaggt
    14161 acagattaac aacgttaaca ttctcggtcg tcagaacacc ttctttgtca ccaacagcgg
    14221 tgtgcagaac cgtctggaaa cgaatcgtca gccgcgtacg ctggtgacca acagctacat
    14281 tgaaggggat gtggatatcg tttctggtcg cggcgcagtg gtgttcgata acaccgaatt
    14341 ccgcgtggtg aactcacgta ctcagcaaga agcgtatgtg tttgcaccgg ctacgctgtc
    14401 caacatttac tacggtttcc tcgccgtaaa cagccgtttc aatgctttcg gtgatggtgt
    14461 ggcgcaactg ggccgctcgc tggatgttga tgccaatacc aacggtcagg tggtgatccg
    14521 tgatagcgcc atcaacgaag gttttaacac ggctaaaccg tgggccgatg cggtgatctc
    14581 taatcgtccg tttgcgggta ataccggcag cgtagatgat aacgacgaaa tacagcgcaa
    14641 tctgaatgac actaactaca accgcatgtg ggaatacaat aaccgcggcg tgggtagtaa
    14701 agtggttgca gaggcgaaga agtaagagca attaactatt tgccggatgc ggcgtaaacg
    14761 ccttatccgg cctacggttc gatgcgattt gtaggtcgga taagatgcgc aagcatcgca
    14821 tccgacaata agtgccggat gctgcgaaaa tgccttatct ggcctacaga ttcgatgcga
    14881 ttcgtaggtc ggataagatg cgcaagcatc gcatccgaca ataagtgccg aatgcgacct
    14941 acattcacat ggcgcttttt acatctgacg gtttttattg aagttaatca aactacccgc
    15001 cttgataatc tcgcgctctt cagcagtcag actttccata tagagcgtaa tttccgttac
    15061 cggcgcatct tcatggatca cataaccttt aaacgtcgta cccggattat ccagcgccgc
    15121 tttaatgcca gggatgtaaa tgtaatcccc cacttcaaag gttggcactt ccgccatctg
    15181 cagcggtaac atcccccagt tgatgacgtt agaacggtag cgtttagtcg cgtactcttc
    15241 ggcaatattc gccagaccgc caatcacacg ctggcagctc gccgcctgtt cacgcgcaga
    15301 accatcgcct ggtttcaccg catataccat gctgccaatt tcagtttgca gcggatcaat
    15361 atgctcctga ccagcaatct gcttaatgcg cgcaaacacc tctgtcagct cgctgacatt
    15421 ccccgccaga cgctgatttt ccagctcagc agtcgcttta cttctgccaa cataaccggg
    15481 atcgcggcgt gacagggtaa actccgccag accaatcgga tttgaacgat aagaagaggt
    15541 ttcaccggaa ggaatcagtt cgtcggtggt ggtcacttcg tcgaggatct tcgagcacac
    15601 tttcaggacg atattgtcag tcagcgcacc caattccggc cagtctttaa tgttcggtcc
    15661 gtaaatcagc ggttgctgag ttgccccttt cacaaagccc tgataaacac ggtttttata
    15721 cggcgttaca tcgaaggcgt actccggcac gttgtcccag caatcaagtt cgctggcaga
    15781 ggttaaatag ccaccgtttg ccgcagtcgc agcgatagaa cgagcgtcca tcaacgccac
    15841 cgctgacatc tgcccattag ctggcttaga gccttcgcgg ttcgggaagt tgcgcgtggt
    15901 gtggcgaata cttaatccgt tgttgattgg cgtatcgccc gcgccaaagc atgggccgca
    15961 gaacgcggtt ctgatgattg cgcctgcgcc aatcaaatct gctaccacac cttttttggc
    16021 gagatccata aacaccggct gtgatgacgg gtaaactgcc agcgagaagg tgtcattgcc
    16081 acaggattga ccgcgcagtg cattcgccgc cgcgatgacg ttttcgtagt taccgccaga
    16141 acagcccgcg ataatcccct gctgcacttt caggcgacca ttttccactt tatccagcag
    16201 cgagagtttg gctttaccgt gcgccacgcg ttcggactca atttcaatct cacgcagaat
    16261 gtcggtcagg ttctggttca gtgtgtcgat ttcatacacg ttgctcgggt ggaacggcag
    16321 cgcaatcatt ggtttgatgg cgcttaaatc aacgctgatg cagccatcgt agtacgccat
    16381 cggttgaggg ttaagctggc agtaatcctg gccgcgaccg tgcagcgcca gccagttatg
    16441 gacttcttca tcggtttgcc agacagaact taaacaggtc gtttcagtgg tcataacgtc
    16501 aacgctgtta cggaaatcgg tagagagcgc agcaacaccg ggacctacga attccatcac
    16561 tttgtttttg acgtagccgt ttttgaacac ggcaccgatg atagccagcg ccacatcctg
    16621 cggccccaca tacggcgctg gttttccggt cagatgcacc gcaacaactc ccggatagtc
    16681 gatatcccag gtgtcattaa gcagctgttt taccaactca ccgccgccct caccgactgc
    16741 cattgtccct aatgcaccgt aacgggtgtg gctgtctgac ccgaggatca ttttgccgcc
    16801 gcctgccatc atctcacgca tatattgatg gatgaccgca atatgcggag gcacaaaaat
    16861 accgccataa cgctgagctg ccgataaacc aaaaacatgg tcatcaccgt taatggtgcc
    16921 gccgacggcg cagagtgagt tatggcagtt ggtcagcaca tacggcagcg ggaaacgttc
    16981 cattccggac gctttagcgg tctgtacaat accaacaaag gtaatatcgt gagaggctaa
    17041 tgaatcaaac ttaattttaa gtttatccat atttccggac gtattatgag aagagagaat
    17101 agaccaggca atagtgcctt ttttggcttc ttctttttta atttcgccgg tgaaatgttc
    17161 ttcggcaatt atttcgttat tactggcgag aaacacgcct ttttcagata acttgatcat
    17221 caaatgctcc agatatctgc gcaccgtgaa gtgcgcagaa agataataaa tggatatact
    17281 ctaaataatt cgagttgcag gaaggcgaca agtgagtgaa tccccaggag cttacataag
    17341 taagtgactg gggtgagcga acgcaaacgc agcacatgca acttgaagta tgacgagtat
    17401 atttacagca tgttccagcc gatcagcata ttccaccacc acacgccgag ggtgatatgc
    17461 accaggaagg ttaatatcgt cagtaccgca ccgaccaacc accaggattt aatatcgtta
    17521 taacccacgc caaagatgac cggacctgcc gcgccgccat agtgagtaac catgccgcca
    17581 taggagttgg agaacaacag tgccagcgcg gttaacatta acggtgcgcc ggagacgttc
    17641 gccagcatgg caaataccgg taacatagca acgatatagg cactaccgga agcgaagaaa
    17701 taacgcacga taatgctgag gaaaataata acgaagaaag caacgttacc gtgaccatca
    17761 aatgccaggt tatttttaaa gacttcagct aaccattcga agaatttaac tttcgataat
    17821 aaggagctta agccgataat accgccgtac cagattaagg tattccagcc gcctttattt
    17881 ttaaccacgt cttcccaggt aacgataccc agcagcatgg ttgccataac aacgattgcc
    17941 acggtggatt catcaacccc cagagactta ctgaaaatcc agcccagcag cgccagcaca
    18001 aagacaccga gcagcatttt ttcgcggatt ttcatcggtc ctagttcggc aaggcccgct
    18061 ttagcgatgg ttttgttatc caccttctta atttctggtg gatacatggt gtaaatcacc
    18121 agcggggtga ccagcagcat aatgatgccc ggcaatccgg ctgccagcgc ccatccaccc
    18181 cagctaattt gcaggtgcag aatgtcgttg atcattttca gcgccagaat gttccccgcc
    18241 attgcggtaa agaacatata gctggtggtt ttggtgacca tgtaaatgga catcatcagg
    18301 taatgtccga cacgacgcgg acttttttcc ggttcggacc ccaaagccac cgccacgctg
    18361 ttgatgatcg gtaacacaat gccgcccgca cgcgcggtgt tagacggtgt tgccggagcc
    18421 agtaccagat cgaggaatac cgtaacgtaa cccagaccca gcgtggtgtt accgatttta
    18481 ccaatcagca gataggcaat acgtttacct aaaccggtgg tcacaaatgc ggcgcttaag
    18541 gtaaacgccg agaacaccag ccaggtggta cctgaagagt aaccgcttaa tacggcggtg
    18601 gttttaaacg caccgtcgga taagttaccg accaccacca ttgaggcagc aacggcaatt
    18661 aacagtacga caggttccgg gaaaggcttg ataaccagcc ccacaatggc cgtcaggtaa
    18721 ataccaaaaa gcacccacgc cagttcgctt aatcctgccg gagctggcat aaagccaata
    18781 atacatggga tcgctaatat cagaattagc ttccataacg atttcttatt catttaaaat
    18841 cctgttcttt ctgtatgact catacagaag tcctttattg attgtcctta tcctgacaac
    18901 gaattttttc agggaagata aacttcaccg gaaaatattt ttctggtcgt ccgaataaca
    18961 gatgcgcgta acgtcgtggc atcctgacct tcattactta aatgaacgtc gagcgcacca
    19021 ctggggtgtt caatattgat attgccgtat cctacagaag ggacgatttg tcgggtgacg
    19081 gtgccttcca atgcacaact actggaaata gcaatagcac cggttatcgc cagcgcgcga
    19141 tggcaagaat gcggcataaa ataacgcaca ttaattgccc cgcctttctg cgctggagaa
    19201 ataagcacag gtttagggat aaccatatta ctgacatcac ctaagcccat tgctttaccc
    19261 gcttgtagac ggatagattc aatgcgggct aataatgctt tgtcggcatc cagttccgcc
    19321 ggtaattcat aacctgtttt tcccagatat tcagccggaa taatgacgac tggcatcgcc
    19381 atatcgatac aggtcaccgg gacatcgtca aaataatcaa tctgattatc agtcgggaaa
    19441 acttttccgg ttttggttcc agcggcattc aggaaagtga gcgcaaccgg tgcggcagta
    19501 cccggtacgc cgtcaattct ggcgctaccc tcgtactcga caacaccatt tggcgtttgc
    19561 acatcagctt cgatgaacgt acccgtattg acgttgcgga tacgtacgcg agtaactggc
    19621 gaagtcgctg caatcaaacc attttcaatg gcgaatgccc caacaccaga cagcatattg
    19681 ccgcagttag gcgtggtatc gacacgttgc tcatgaacga ttacctgcgc aaacagataa
    19741 tcgacatcag cacgcggatc gctggaacgg ctaataatgg cgactttact ggtcagtgga
    19801 ttaccgccgc caataccgtc aatttccaga tcgttaccgg aacccataat tgccatcaat
    19861 attttatcgc gctgcgtttg atcttcgggt aaatgttccg ctaacaggaa cgcgcccctc
    19921 gaggttccac ctcgcatcat cacgcagggt atttttttca taataatgcc ctcaatttaa
    19981 aaacataact tattgcgata tggttacatt aagggcaaag catctctaat gactttagct
    20041 caacgattaa taacattttt tcattaattt aagttggcta aatgcagtta aaatttttaa
    20101 cggccagcca cccaaaatcg tcgcttccat tttccggagt aataatgaag catgaattat
    20161 caagtatgaa ggcatttgtc atactggcag agtccagttc gtttaacaat gccgctaaat
    20221 tactcaatat tacgcaacct gcattaacgc gaagaataaa aaagatggag gaagatttac
    20281 atattcaact atttgagcgt acaactcgca aggttacgtt aacaaaagca ggaaaaaggt
    20341 tgctcccgga ggcgcgggaa ttaataaaga aattcgatga aacgcttttt aatattcgtg
    20401 atatgaatgc ttatcatcgt ggtatggtga cgttagcgtg tattccgacc gcagtatttt
    20461 attttttacc gctggcaatc ggtaaattta atgagctgta tcccaatatt aaagtgcgga
    20521 ttctggaaca aggcacgaat aattgcatgg aatcggtgct gtgtaacgaa tcagactttg
    20581 gcataaacat gaacaacgtc acaaattcat ccattgattt taccccactg gtcaacgagc
    20641 cgttcgtgct ggcctgtcgg cgtgaccatc cattggccaa aaagcagctt gtagaatggc
    20701 aggagttagt gggttacaaa atgattggcg tacgctcctc cagcggcaac cgactgttga
    20761 tagaacaaca gctggccgac aagccctgga agctggactg gttttacgaa gtgcgtcatc
    20821 tttcgacgtc attaggactg gtggaggcgg gactggggat ttcggcgctt cctggcctgg
    20881 caatgcctca tgcgccctat tcttcgatta ttggtatccc attggtggaa cccgttatac
    20941 gtcgaacatt gggaataatt cgccgtaaag atgccgtact ttctccagca gcagaacgct
    21001 tttttgcctt gctgattaat ttatggactg acgataaaga caatttgtgg accaatatcg
    21061 tcgaacgcca gagacacgcg ttacaggaga taggttaata caccggcgca ggagagactg
    21121 cgccgggtaa atcagcggtt agtgtgcgtt aaccaccacc cacattggtc cctgcccgac
    21181 cgcatagcgg cctttttcat gcagtagccc ctgctcgcca acaatttcgt ataccgagat
    21241 gtggtgagat ttttgcccag cggcaatcag atacttgccg ctgtgatcaa cattgaagcc
    21301 gcgcggctgg gtttccgttg gctggaagcc ttctttactc aacacgctgc catcttccga
    21361 aacgctgaag acagtaatca ggctggcagt acggtcacag gcatataagt gacgcccatc
    21421 cggggtgata tgaatatcag ccgcccaacg ggtatcggag aagttttccg gcatcatatc
    21481 cagcgtctgg acacattcga tattaccgtg cggatctttc agttcccaga catccactga
    21541 gctgtttaac tcattgacgc aatacgcata ttgttcgttt ggatggaata ccatatgacg
    21601 cgggccggcc ccttcaacgg tggtcacttc cgcagggtcc tgcgccacga gatgaccatc
    21661 atcgctgacc gtaaacaggc aaatgcgatc ctgctttaat gccggaaccc acagcgtacg
    21721 gttgtccggt gagatattgg cggaatggca accgtccagc ccctcgacca catcgacgac
    21781 gcccactggc aggccatctt ccagacgcgt tacgctcacg ttacccgcat tgtaagaacc
    21841 tacaaagaca aactgcccct ggtgatcggt ggaaatatgc gtcggactac ccggcagcgc
    21901 agactctgcg gcaaaggtca gtgcgccatc gtccggggcg atacgatacg ccaggacgcg
    21961 aaactcaggg cgaacaccaa catagagata acgtttgtcc gggctgacca ccatcggctg
    22021 cacctgcccc ggcacatcga caacctgtgt cagcgtcagt gcgccttcat gattcaggtt
    22081 ccagacgtga atttgctggc tctcagggct ggcgatataa actgtttgct tcatgaatgc
    22141 tcctttgcat ttagcaactg actgtaacag ctaaaattag tcgcttttgg cggtgaatgc
    22201 tgaattaaaa cgcagaattt tgtccgatag tggtatcggt gtaccattcg cgcagacgaa
    22261 aattcttaac cttaatctgg aatacgccat gaccacacgc gtgattgctc tcgacttaga
    22321 cggcacctta ttgaccccga aaaagaccct gcttccttca tcgatagaag ccctggcccg
    22381 cgctcgcgaa gcaggctatc aattaatcat cgtcacaggt cgccatcacg tcgctattca
    22441 tcctttttat caggcgctgg cgctggatac acctgctatt tgctgtaatg gcacctattt
    22501 gtatgattat catgcaaaaa ccgtgctgga agcggaccca atgcccgtta ttaaagccct
    22561 gcaactcatt gagatgctga atgaacacca cattcacggt ctgatgtatg tcgatgatgc
    22621 aatggtctat gagcacccga ccgggcatgt cattcgcaca tctaactggg cgcaaaccct
    22681 gccgccggaa cagcgtccga ctttcacaca agtcgcttct ctggctgaaa cggcgcaaca
    22741 agttaacgcc gtatggaagt tcgccctgac gcacgatgac ctgccgcaat tgcagcattt
    22801 tggtaagcat gtcgaacatg aactgggact ggagtgtgaa tggtcctggc acgatcaggt
    22861 tgatattgca cgcggcggca acagcaaagg taaacgtttg acgaaatggg ttgaggcgca
    22921 aggttggtcg atggaaaacg tcgtggcatt cggcgataac tttaatgata tcagtatgct
    22981 ggaagccgct ggtacaggcg tggcgatggg caacgccgat gacgcggtaa aagcgcgcgc
    23041 caacattgtg attggtgata acaccaccga cagcattgcc caattcattt atagccacct
    23101 gatttaatca ggcggttatc gacacacttt taatttgcgc gtacagccac aggccaggtt
    23161 tgatcgccag ttcatccctg gcccacgggc tgatacgcgc ccacagcgtt ttaccgccga
    23221 cttccagttc cacttccacc tggccgttgt cgtcataact attaacaact tttgcccgca
    23281 atacgttacg aatgctggtt tgctgcggcg gttgtaaaac cagagaaaca tcggaagcct
    23341 gaatgcggat acgtagcgca gcttgcagcg gttcgtccag cttattgacc cacaaatgct
    23401 gatcgcccag cgccagcgcg gtcatcgcgt aatgcggatg atgctcaagc accgtcactt
    23461 tcagaatgct actttgttgc tctttcggca gccacggatt catcacgcta ctgccccaca
    23521 cttcctccag cgcgccaaag gctttcacct gaccgttttc cagtaccatc actctgtctg
    23581 ccagatggag gatctcatcc agcgaatggc tgacatacaa catcggaatg ttgatttccc
    23641 gtgtcagccg ttgcagataa ggcaacagtt cgcgtttacg cggaatatcc agtgacgcca
    23701 gcggttcatc cagcaacagc aattccggtg ctgtcagcaa agcccgacca atcgccacgc
    23761 gctgtttttc gcctccggac aggctgcctg gtaaacggtc aagcaacggt tcaatgccta
    23821 aaagcgccac cagcttatcg aactgatcga ccatactttt cgacatgccg tagcgcagat
    23881 tgccacgcac tttgtaatgc gggaacagcc gcgcatcctg aaaaacatag ccaacgcgac
    23941 gcttttccgg cgtcaggcag ataccttttt cggcatcatt tagtacccgc ccattgagga
    24001 caatccgccc tttttgcggg cgcgtcagtc cactgatggc gttaatcagc gaagtttttc
    24061 cggcaccgga gacgccaaag atagcagtga tgccattggc gggcagcgtt tcattaatag
    24121 tcaggcaatg gttgcccaac gtctgggaaa aattcagttc cagcatgatt agcgccccgc
    24181 ccgttcacgg ctgattctgg ccagccattc tgaaatcaac agggagatca tcgccagcgc
    24241 aatagaaata atgcacagtc tcgccgctcc actttccccg ccgggggtct ggatcagggt
    24301 atacatggca gaaggaatgg ttcgcgtttc accaggaatg ttcgacacaa aggtgatggt
    24361 tgcaccaaac tcaccgagag aacgagcaaa agccagtacc gtaccaacaa taattcccgg
    24421 taaggtcagc ggtaacgtga tagtaaagaa aacgcgccag cgcccggccc ccagtgttct
    24481 tgcggcctgt tccagtttga catcaacccc ttccagcgcc agacgaattg cccgcaccat
    24541 cagcggaaac gacatgacgg cggcagcgag aaccgcgccg cgccagctaa aggcgaaggt
    24601 aataccaaac cagtcataca gacgttcacc gataaatccg cgccgtccca tcgaaactaa
    24661 taataagtaa ccgacgacca cgggcggtaa caccagcggt agatgcagta cgctgtcgag
    24721 cagagctttg cccggaaacg tgcaacgcac cagtaaccag gcaaaaaaga tcccaaacgg
    24781 caggctaaac agcacagcca gggaagaaac tttcaggctt aataaaactg cctgccattc
    24841 tggatcggtc agtatcatta cttgattgta aatccgtaac gtttaaagat ttccgctgcc
    24901 tgcggtccct tcagataatc ataaaatgct ttcactgtcg cattgttatg cccttccaca
    24961 acagcaaccg gatattccac ttttttatgt gaatcttccg ggaaggtggc aaccactttt
    25021 acccctttgc tggcaactgc gtcagaaccg tagacaatgc ccagaggcgc ttcgttacgt
    25081 tcgaccagcg ccagcgcccc acgaacatct tccgctgggg ccagtttcgg agagagcgta
    25141 tcccatgcgc ccagtttttg cagtgcttct tttgcataaa tgccagcggg aacatgttcc
    25201 ggatcgccaa ccgccaggcg accgccattc agcagtgaag tccagttggt tttgctgtcg
    25261 atggtgaaat ctttctgcac gctggctttc ggtgctacaa cgaccaggct attgccgagc
    25321 agtgtctgac gcgtagctgt atcgatcgct tttttatcaa ccgcataatc catccatttc
    25381 tgatcggcag aaataaacag atccgcaggc gcacccgctt caatctgacg ggcgagagta
    25441 gatgacgaag cgaaagaaga aaccacatcc acgcctttct cttttttaaa ctgcgtagca
    25501 atgtcctgca ttgcgttagt cagtgatgcg gcggcgaaca ccgtgatttt cccttcatct
    25561 gccagtgcat tgccagcaac agcgaaagag agtgccgccc cggcaaacaa gttcaaccat
    25621 ttacgagcca ttggtaaccc cttaatgtaa cgttatgtag gcgacaatat aacgactaac
    25681 tcggaatttt cccagcagtt attgctaacc tttgtgaggt agataagaaa aaatatcggc
    25741 aggaaaagca ggaagttgag aaaaagaaaa tgcccgacta agcgggcatt cagggaatca
    25801 atgattttgt ccgggctggt cttttttacc aacaccagaa aagatgttga atacttcacc
    25861 aagaccgtaa atcagaccca ggatgatggc catcacgaca ggtaccatga ttacggcgaa
    25921 taccagactt tttaataact ctaacatggt caactccaga tatagtcatg agactattct
    25981 aaccgctaag cacagaaaag cactcccctt ttgtgcggtc agctttgcgt ggcgttcgtt
    26041 ttccgtcaca ataagacttt tgccaggaca ttgttatgca ggccgaaatc cttctcaccc
    26101 ttaagctcca acaaaaatta ttcgccgacc cgcgccgcat ttcgctacta aaacacattg
    26161 cgctttccgg ttccattagc cagggagcga aagatgccgg tattagctat aaaagcgcct
    26221 gggatgccat taacgagatg aatcagttaa gtgagcatat tctggtcgag cgcgcaacag
    26281 gcggtaaagg tggcggcggc gcagtactga cccgctatgg tcagcgactg attcagctct
    26341 atgacttact ggcgcaaatc cagcaaaaag cctttgatgt gttaagtgac gatgacgccc
    26401 tgccgctgaa cagcctgctg gccgcgatct cacgtttttc actgcaaacc agcgcccgta
    26461 accagtggtt cggtaccatc accgcccgcg atcatgatga cgttcaacag catgttgatg
    26521 tcttactggc tgacggaaaa acacgcctga aagtcgcaat taccgcacaa agcggcgcgc
    26581 gtctggggct ggatgaaggc aaagaagtgt tgatattgct aaaagcgccg tgggtaggta
    26641 ttactcagga cgaggcggtc gcgcaaaacg ccgacaacca attaccgggt attattagtc
    26701 atattgagcg cggcgcagag cagtgcgaag tattaatggc gctacccgac gggcaaacac
    26761 tgtgcgccac agtgccggta aatgaagcga cttctcttca gcaagggcag aatgtcacgg
    26821 cctactttaa tgccgacagc gtgattatcg ccacgctgtg ctaagcgtgt tgacaatttg
    26881 ttatgaaaca cgtatccctg tcagtaatcg ctgcacaaaa tgggatataa aatgtcatcg
    26941 ttgcaaattt tgcaaggcac gtttcgtctt agcgacacaa aaacgctgca attgcctcag
    27001 ctaacgttaa acgcgggtga tagttgggcg tttgtcggtt cgaatggaag cgggaaatcg
    27061 gccctggccc gcgcgctggc gggggaactt ccgcttttga aaggtgaacg gcaaagccag
    27121 ttttcccaca tcactcgtct ctccttcgag caattgcaaa agctcgtcag cgacgaatgg
    27181 caacggaata acaccgatat gctcggccct ggcgaagatg acaccggacg cactacggct
    27241 gagattattc aggatgaagt aaaggatgca ccgcgttgca tgcaactggc gcagcagttc
    27301 ggtattaccg ccctcctcga ccgacgcttt aaataccttt ccactggcga gacgcgaaaa
    27361 accctgctgt gtcaggcgct gatgtcggag cctgacttgt tgattcttga tgagccgttc
    27421 gatggcctgg atgttgcctc acgtcagcag ctggctgagc gactcgcctc gttacatcag
    27481 tccggtatta ctctggtact ggtgctcaat cgcttcgatg agatcccgga atttgtccag
    27541 tttgctggcg tgctggcgga ttgtacgtta gcggaaacgg gcgctaaaga ggaactgctc
    27601 cagcaagcac tcgtcgcgca actggcgcat agcgaacagc ttgaaggtgt gcaactgccg
    27661 gaaccggatg aaccttcagc acgtcacgcc ttacccgcca acgaaccgcg cattgtgctg
    27721 aacaatggcg tggtttctta taacgatcgc cccattctta ataaccttag ctggcaggtg
    27781 aatccaggcg aacactggca aattgtcggg ccaaatggtg cgggaaaatc gacgttatta
    27841 agcctgatta ctggcgatca tccgcaaggt tacagcaacg atttgacgct tttcggccga
    27901 cgtcgtggca gcggcgaaac catctgggat atcaaaaagc atatcggtta cgtcagcagt
    27961 agtttgcatc tggattaccg ggtcagcact accgtgcgta atgtgattct ttctggctat
    28021 ttcgattcga ttggcattta tcaagccgtt tcggatcgcc agcaaaaact ggtgcagcag
    28081 tggctggata ttctcggcat tgataaacgc acggctgacg ctccgttcca tagtctttcc
    28141 tggggacagc agcgtctggc gctgatcgtc cgcgcactgg tgaaacatcc gacgttgctt
    28201 attctcgatg aaccactaca ggggcttgat ccgctcaatc gccagcttat ccgccgtttt
    28261 gttgatgtgc tgattagcga aggtgaaacg caattgttgt ttgtttcgca ccacgctgaa
    28321 gatgcgcctg cctgtattac ccatcgcctt gagttcgtgc cggacggtgg actctatcgc
    28381 tatgtgctga caaaaatata ttgagtcggt agtgctgacc ttgccggagg cggcctaagc
    28441 gccctctccg gccaacggtt cgacgcatgc aggcattaaa ccgcgtcttt ttttcagata
    28501 aaaagcgcaa tcattcataa accctctgtt ttataatcac ttaatcgcgc ataaaaaacg
    28561 gctaaattct tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt
    28621 gttatgctat ggttatttca taccataagc ctaatggagc gaattatgag agttctggtt
    28681 accggtggta gcggttacat tggaagtcat acctgtgtgc aattactgca aaacggtcat
    28741 gatgtcatca ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag
    28801 cgtttaggcg gcaaacatcc aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg
    28861 accgagatcc tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc
    28921 gtgggcgaat cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggcactctg
    28981 cgcctgatta gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc
    29041 accgtttatg gcgatcagcc caaaattcca tacgttgaaa gcttcccgac cggcacaccg
    29101 caaagccctt acggcaaaag caagctgatg gtggaacaga tcctcaccga tctgcaaaaa
    29161 gcccagccgg actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg
    29221 tcgggcgata tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc
    29281 caggttgctg taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa
    29341 gatggtactg gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg
    29401 gcgatggaaa aactggcgaa caagccaggc gtacacatct acaacctcgg cgctggcgta
    29461 ggcaacagcg tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaat
    29521 tatcattttg caccgcgtcg cgagggcgac cttccggcct actgggcgga cgccagcaaa
    29581 gccgaccgtg aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc
    29641 tggcactggc agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa
    29701 tttaatcccg ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg
    29761 gtttcaccgc accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag
    29821 gtgttacctg cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc
    29881 gataaaaacc ccgattacac cgggacttac gttttcacta atgactttgc ggctttgatg
    29941 tctgacacgc cagatgcgcc agaaagtcac gatccgctga tgcgttgcca gagcgcgcgc
    30001 ggcaccagcc gggtgatctg cttttcaccg gatcacagta aaacgctgcc agagctcagc
    30061 gttgcagcat tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga actggggaaa
    30121 acgtacccat gggtgcaggt ttttgaaaac aaaggcgcgg cgatgggctg ctctaacccg
    30181 catccgcacg gtcagatttg ggcaaatagc ttcctgccta acgaagctga gcgcgaagac
    30241 cgcctgcaaa aagaatattt tgccgaacag aaatcaccaa tgctggtgga ttatgttcag
    30301 cgcgagctgg cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgtcgtg
    30361 ccttactggg ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttacgg
    30421 atcaccgatt tgaccgacgc ccagcgcagc gatctggcgc tggcgttgaa aaagctgacc
    30481 agtcgttatg acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgcg
    30541 ccatttaatg gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg
    30601 ctgcgctccg ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag
    30661 cgagacctga ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt
    30721 cgcgaatccg gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac
    30781 gcatttggct accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt
    30841 gaacacaccg actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg
    30901 atcagttgtg caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat
    30961 cagctcgacg agttttccct cgatgcgccc attgtcgcac atgaaaacta tcaatgggct
    31021 aactacgttc gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggcgtg
    31081 gacatggtga tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg
    31141 gaagtcgcgg tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa
    31201 atcgcgctta acggtcagga agcagaaaac cagtttgtag gctgtaactg cgggatcatg
    31261 gatcagctaa tttccgcgct cggcaagaaa gatcatgcct tgctgatcga ttgccgctca
    31321 ctggggacca aagcagtttc catgcccaaa ggtgtggctg tcgtcatcat caacagtaac
    31381 ttcaaacgta ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt
    31441 gcgcgtttct tccagcagcc agccctgcgt gatgtcacca ttgaagagtt caacgctgtt
    31501 gcgcatgaac tggacccgat cgtggcgaaa cgcgtgcggc atatcctgac tgaaaacgcc
    31561 cgcaccgttg aagctgccag cgcgctggag cagggcgacc tgaaacgtat gggcgagttg
    31621 atggcggagt ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac
    31681 actctggtag aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc
    31741 ggcggatttg gcggctgtat cgtcgcgttg atcccggaag agctggtgcc tgccgtacag
    31801 caagctgtcg ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt
    31861 aaaccatcac aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc
    31921 agccgtaccg actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact
    31981 ggggtgcgac tttactttca gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc
    32041 tgctcggctg tgccagcccg gaatgctatc aggatcaggc cgcgtttctg ggggcctcta
    32101 ttggtcgtta tgccaaccgc atcgccaata gccgttatac ctttgacggt gaaaccgtga
    32161 ccctttcgcc aagtcagggc gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac
    32221 gtcgctggca gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg
    32281 atggcgatca gggcttcccg ggtaatctcg gcgcaacggt gcaatatcgt ctgaccgacg
    32341 ataaccgtat ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga
    32401 ctaatcacgt ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc
    32461 agattctggc ggacgaatat ctgccggttg atgaaggcgg cattccgcac gacggcctga
    32521 aatctgtcgc cggaacgtct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc
    32581 ttgccgacga cgatcagcgc aaagtgaaag gttacgatca cgcattcttg ttacaggcca
    32641 aaggcgatgg caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga
    32701 aggtctacac caccgctccg gctcttcaat tctactccgg caacttcctc ggcggcacac
    32761 cgtcgcgggg aaccgaacct tacgccgact ggcaagggct ggcgttggaa agcgagtttc
    32821 tgccggacag cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg
    32881 aagagtattc cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca
    32941 tgagggcttt atcttcatat tgttcaaatc accagcaaac accgacatat ttgcaactca
    33001 atattcacaa caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc
    33061 tgttgcttcg tcgcggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt
    33121 aagtatatgg ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa
    33181 gaaaaccgtt tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca
    33241 aaagcagcag gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct
    33301 gtgctgaaac gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg
    33361 ctgcccgttg agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg
    33421 aacaaagcgg aaactgctga aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt
    33481 tttgcagtga ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg
    33541 cgttacgcga aactgagcga gaaagaactg ccgctgacgg aaagcctggc gctgaccatt
    33601 gaccgcgtga tcccttactg gaatgaaact attctgccgc gtatgaagag cggtgagcgc
    33661 gtgatcatcg ctgcacacgg taactcttta cgtgcgctgg tgaaatatct tgataacatg
    33721 agcgaagaag agattcttga gcttaatatc ccgactggcg tgccgctggt gtatgagttc
    33781 gacgagaatt tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcagcg
    33841 aaagcagcgg cggttgcaaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaatgag
    33901 aaagccgact gcaagtgagt cggctttttt gttgctaaca atggagcaca ttgcctgatg
    33961 cgacgctgcg cgacttatca ggcctgtggt gattcatcgg atacgccact ctgacggcgc
    34021 atccgacaat taaaccttac ccgcgacgcg cttttactgc attcgccagt tgacgtaaca
    34081 gagcatcggt atcttcccag ccgatgcagg catcggtgat gctcttaccg taggccagcg
    34141 gctccccgct ctcgaggctc tgattgcctt ccaccagatg gctttccacc atcacgccaa
    34201 taatggcctt ttcgccaccg gcaatctgct ggcaaacgtc agcacaaaca tccatctgct
    34261 ttttgaattg tttggacgag ttagcatggc tgaaatcgat catcacctgt gctggcaggc
    34321 ctgctttgtt cagcccttct ttcacttcag caacgtgctt cgcgctgtag ttaggctctt
    34381 taccgccgcg cagaatgata tggcaatcgc cgttaccgct ggtattcaca atcgccgaat
    34441 gcccccattt cgttacggac aggaagcagt gcggcgcacc ggcggcatta atggcatcga
    34501 tagccacttt aatcgtaccg tcggtgccat ttttgaagcc gaccggacaa gaaagccctg
    34561 atgccagttc gcggtgcacc tgcgattcgg tggtacgtgc gccaattgcg ccccagctca
    34621 tcaggtcagc gagatattgt ggggtgatca tatcgagaaa ctcacctgcc gctggcagac
    34681 cgctgtcgtt aatatcaagc agcaatttac gggctatacg cagaccgtcg ttgatctgga
    34741 agctattatc catatgcgga tcgttaatca gccctttcca gcccaccgtg gtacgcggct
    34801 tttcaaaata gacgcgcatt acgatttcca gctcatcttt cagctcttca cgcagcgcca
    34861 gcaagcgagt ggcatactct tttgccgcga caggatcatg aattgagcat gggccaatca
    34921 caaccaacag gcgatcatca ttacctttca ggatcttatg gatcgctttt cgggcatggg
    34981 caaccgtatt cgcggcattt tcagtagcgg ggaatttttc cagcaatgcg acaggaggaa
    35041 gtaactcttt gatttctttg atgcgtaaat cgtcgttctg ataattcatg tctgttccag
    35101 tgttgccata cttatcttag tgaatgcaat acttccaatc tatatcttcc gtcagaatgt
    35161 gtaaacgggg ttttacacta tgaacggatt aatcctggaa ttagcaagaa aaacgccaga
    35221 ttgtcgcgaa aaacgagatc tctcctacaa tttctaactg taactccttt gtttgttaat
    35281 tatttcaaga ttctctgctg cgtttcataa cctggctgaa atcttaaacc aatgccttat
    35341 attcacctgc aaatgcactg ttggaagagg ttatccgaca taacgaccat aacaggagca
    35401 tcctatgaaa atgacaaaac tggccacatt atttctgact gccactctaa gccttgccag
    35461 cggtgccgca ctggctgccg atagcggagc gcaaactaat aacggccagg caaacgccgc
    35521 agctgatgcg ggccaggtag cccctgacgc ccgtgaaaat gtcgcgccaa ataacgtcga
    35581 caataacggg gtaaataccg gttctggcgg cacaatgctg cattcggatg gttcttcaat
    35641 gaacaatgac ggaatgacca aagatgaaga gcacaagaac accatgtgca aagatggtcg
    35701 ctgtccggat atcaataaaa aggtacaaac cggtgatggc atcaataatg atgtcgatac
    35761 caaaaccgac ggtaccacgc agtaaccctg caaaatagcc aataatggga gagcggagtc
    35821 tctccctttt aatttttcac ttctaaaagc agtatgatgt cataaagtta agtgagaata
    35881 attaagggat gactcgtcat ggcgcactca cactcaaaca cgtcctcaca cctgccagaa
    35941 gataataatg ctcgtcgctt gttgtatgct ttcggcgtga ctgccgggtt tatgctggta
    36001 gaagtcgttg gtggttttct ttctggttct ctggcattgc tggccgatgc gggtcatatg
    36061 ttgaccgata ctgccgccct gcttttcgcc ctactcgccg tgcaattttc acgtcgtcct
    36121 cccaccattc gccacacttt cggttggcta agactgacca cgctcgcggc gtttgtgaat
    36181 gccatagccc tcgtggtaat taccattttg attgtctggg aggcgataga acgcttccgc
    36241 acgccgcgtc cggtcgaggg cggcatgatg atggcaattg ccgtggctgg gttgctggca
    36301 aatatacttt ctttctggtt acttcatcac ggcagcgaag agaaaaacct caacgtacga
    36361 gcagcggcac tgcatgtgct cggggacctg ctgggttcgg ttggggctat tattgccgcg
    36421 ttgattatta tctggactgg ctggacacct gctgacccca ttctctcgat actggtgtcg
    36481 cttctggttc tgcgtagcgc gtggcgactg ttgaaagata gcgtgaatga attacttgaa
    36541 ggtgcaccgg tatcgctgga tatcgctgaa ctgaagcgtc gtatgtgccg ggaaatcccg
    36601 gaagtccgca atgtgcacca tgtacatgta tggatggtag gcgagaagcc ggtgatgacg
    36661 ttgcatgtgc aggtgatccc gccacacgat cacgatgcct tgttggatca gatccaacac
    36721 tacctgatgg atcactatca gattgagcac gccaccattc agatggaata tcaaccttgt
    36781 catgggccag actgccacct gaatgaaggg gtgtctggcc attcacatca tcaccattaa
    36841 tgggacagcg cgcgtgagcc tctttcacgt gcgctgttga tccacatccg gctgccgttg
    36901 agcgcaataa aggtcaggat gatgtactcc agagacatgg cgtaaacgcc ctgaagtgca
    36961 aaaataacga cgctaatcac gttaataatc acccacaaca gccagttttc cacatactta
    37021 cgcgtcatca gaatcattgc cacgatagat aacaccatca tgcatgaatc ccagaacggg
    37081 aaagcgtccg gttgcagttc aggcatcaca acctgtaatc ctaatgcttg catgatcatg
    37141 actgccacgc gggtcaaaaa tgcaaacacc ggattgataa ataccgtcat cagaccaatc
    37201 gaaacaacgc aaaccgccaa ccagctgagt gccttcggca atggcaacca gcgaattttc
    37261 aactccgcct cgttctgact ggtttgtcgc gaccacgcat accaaccgta aatattcgcg
    37321 gcaaagaaaa acacctgtaa tagcaggctg gcatacagct gaatctgaaa gaaaataatg
    37381 ccaaacaagg tgacgttgat caggccaaag aagtagttgc tgatcttctc cagactggca
    37441 aggccaatac acagcaaccc ggcgatcgtg cctaccgctt cgatccatga gagatcataa
    37501 ccgcctgccc ctattggtat atgtaccagg atattctgca cactaaaaaa atccatcttt
    37561 cccccaaagc gtaacgttac gccttattat tcgttatcca cgtagtgtag ccgcaaaatc
    37621 cagcatacga ttgagcggca ccagcgccct ctctcgcagc ctttcttcaa catgaacctc
    37681 gtgattgctt ccttcctgtt ctaatgcctc tgcgatggcc tgaaggccat tcatggccat
    37741 ccacggacaa tgcgcgcagc tgcggcaggt tgcaccctca cctgcggttg gtgcttccag
    37801 taactcttta tctggcaccg cctgctgcat tttgtagaaa ataccccgat cggttgccac
    37861 aataagcctc tgatgtggca atgttttcgc agcagcgatc agttgactgg tggaaccgac
    37921 cgcatccgcc atatcgacaa tagcttgtgg tgattctgga tgcaccagta tggcagcatc
    37981 cgggtattct tcttgcaagc gggttaacgc ctgagtctta aattcatcat gcacaataca
    38041 ggcaccctgc cagcatagaa tgtctccacc cgtctgtttt tgcacgtaac gccccagatg
    38101 tttgtcgggt gcccagatga ttttttcacc caaactatca agatgatcaa taagttcgac
    38161 ggcaatgctt gaagttacca cccaatctgc gcgcgctttt accgcagcag aagtgttggc
    38221 gtagacgacg acagtacgat cgggatgggc atcgcaaaat gcgttaaatt cttcaacagg
    38281 gcagccgaga tccagtgaac attcagcctg aagtgtcggc atcagaattg ttttttccgg
    38341 actgagaatt ttggcggttt ctcccataaa tctcacccca gcgactaaca aagtagaagc
    38401 gggatgcttt gcaccgaagc gcgccatttc cagagaatca gaaatacagc caccggtttc
    38461 ttctgccagt tgttgaattt cgggatcggt atagtagtgg gcaaccatca ccgcattacg
    38521 ttcttttagc agacgtttta tcttctcgcg gtaatacgct ttttcatcaa tgcttaacgg
    38581 cgtcggcttc ggggggaaag gataaatcgc cgtgtctgga tcaaacatta cgctcatctt
    38641 accatctcgt tttacaggct taacgttaaa accgacatta gcgtaatatt cgctgttatc
    38701 ggtgtaattt gttttatatg ctaaacaaga tagtcgaatg gtggggaaaa gtcactcgaa
    38761 ttacgtatga gatgccgaat gccacacttt gcgtcttatt cctccgacgt aaagttgttt
    38821 acgcagcaag cgcggagtat gtcatcaggg tcaagaattg aaattgagag atgcctgaat
    38881 agcaaaaaag cgcctttagg gcgctttttt acattggtgg gtcgtgcagg attcgaacct
    38941 gcgaccaatt gattaaaagt caactgctct accaactgag ctaacgaccc cttgcgggat
    39001 tatgctgctt taatcattca ggatggtggg tcgtgcagga tgactggact tcgtctcgcc
    39061 cttcgggccg ttgctaacgc aacgttatcc ttcacgttct ctaccagtaa ccacctttaa
    39121 attggtgggt cgtgcaggat tcgaacctgc gaccaattga ttaaaagtca actgctctac
    39181 caactgagct aacgacccat atgggtgtta cctgaaagat ttctactcgg aaccaatctt
    39241 taatattggt ggctcgtgca ggatgactcg cttcgctcgc cctacgggcc gttgctggcg
    39301 taacgttatc cttcacgctt aacatctgag tttgatgtta aaactggtgg gtcgtgcagg
    39361 attcgaacct gcgaccaatt gattaaaagt caactgctct accaactgag ctaacgaccc
    39421 gagtggtggg tgatgacggg atcgaaccgc cgaccccctc cttgtaaggg aggtgctctc
    39481 ccagctgagc taatcacccg atactgcgct ggatactaca aactacttcc accgaaccca
    39541 ccgtaattag tggtgggtcg tgcaggattc gaacctgcga ccaattgatt aaaagtcaac
    39601 tgctctacca actgagctaa cgacccggtg gtgggtgatg acgggatcga accgccgacc
    39661 ccctccttgt aagggaggtg ctctcccagc tgagctaatc acccaatact acgctggata
    39721 ctacaactaa ctatgttagt ggtgggtcgt gcaggatgac tcgacttcgt ctcgcccttc
    39781 gggccgttgc tgatgcaacg ttatccttca cgttttacta ccgcttccac cttagcgatt
    39841 ggtgggtcgt gcaggattcg aacctgcgac caattgatta aaagtcaact gctctaccaa
    39901 ctgagctaac gacccacttt ttcgttgctt tcggtttgtt tgatatcccg tggcaacggc
    39961 ggcatatatt actgatttca gacttgagcg caacaaaaat ttcgatgcag atcactcaac
    40021 tgcttatgat tcgcacgaca cgaccagaaa taatgcgact tctggtcgtg tgttatgcat
    40081 tacatcgcgt tcagacgttt ttgtgcctgt ttagcgccat cagtaccagg gtatttactg
    40141 ataacctgct ggtatacggc tttcgctttt gcggtgtcac ctttgtcctg catgatgacg
    40201 ccgactttaa acatcgcatc agcagccttt ggtgacttcg gatagttttt cactaccgaa
    40261 gcaaaatagt acgccgcatc atctttttta cccttgttgt agtttaactg acccagccaa
    40321 taattggcgt ttggcaggta agttgaatca gggtaatttt tgatgaaatt ctgaaatgcc
    40381 accattgcgt catcctggcg ggatttatcc tgcaccagcg caatagctgc attgtaatcc
    40441 gtgtttgcat cgccgctttt caccggcgcg ccagcattcg cagtaccagc atcagctgtc
    40501 ggcgtcgttg atgccgccgc accgctttga tcgctgctgg ttgattgcgc cgctgcacca
    40561 ccgctgctga ggctgtcgat ctgcaacagg atctgctttt gccgctccac gacctgattc
    40621 agttgatact gattttcctg aatctgacca cgcagggaat caatatcgga ttgattatca
    40681 gaaagttgtt gctggagttg ggttaaaagc tggctgtgag cattagaaat acgctcaagt
    40741 tgagtgacgc ggtcttcgac cgagcctgag ccgacactac tgattggtgc ctgagcaaaa
    40801 gcggcccagg gggccgctat accaaccagt aacgacagac tcaatagttg atgtctgaag
    40861 ttactgctca tgcaattctc ttagtaaacc agtaccgcac gacggttttt ggagtatgcc
    40921 gcttcgtcat gacccagtac tgcaggtttt tctttaccgt aagaaacgat ggagatctgg
    40981 tctgcagaaa cgcctttacc ctgcaggtac atcttaacgg cgttcgcacg acgttcaccc
    41041 agggagatgt tgtattccgg agtaccacgt tcgtccgcgt gaccttctac ggtgactttg
    41101 taagacgggt tgctacgcag gaagtttgca tgtgcatcca gcatttgagc gaagtcagaa
    41161 cggatatcgt acttgtccag atcgaagtaa acgatgttgt tctgctgcag ctgttgcatt
    41221 tgcagacgag cctgctcttc ggaagacatg ttgccgttgc cgccgttcgc atccatacca
    41281 gtgccggcac ccagcatgcc ttcgctgccg tcattgctgg cgttcttgtt ggaagaacat
    41341 gccgcaattg ccataacagg cagagcaatc atcagccctt tcagcacttt gttcagttgc
    41401 atttcaatga ttcctttact attcaattaa ttattatcac agatacggcg accaggcagg
    41461 gaatttgacc tgtccatcag ttgccggaag acgcgctttg aaacgcccat ctgtagaaac
    41521 caaattcagc acggatccca tcccctgaga agagctgtag attaccatag tgccgttagg
    41581 tgccagactt ggcgtttcat ccaggaacgt ggacgacaga acttgtacgc ctcccgttgc
    41641 cagatcttgt ttggcaatgt gctgctgccc accattggag ctgaccatta ccataaattt
    41701 accgtcgctg ctgacatccg catcctggtt ctgcgaacct tcccaggtaa tacgttgtgg
    41761 cgcaccgccg ttgatattca ctttataaac ctgcggacga ccggcctggt cagaagtaaa
    41821 tgccaggttc tggctatccg ggaaccaggt cggttcggta ttgttactgc gaccatcagt
    41881 cacctggcgg atctgaccag aagccaaatc cattacgtac aggttcagac taccggtttt
    41941 cgacaaggcg aatgccagtt tgctgccgtc tggcgagaat gcaggtgcac cgttgtgacg
    42001 cgggaatgaa gccacctgac gtacagcgcc atttgccagc gtctgaataa ccagcgcgga
    42061 acgaccgctt tcgaaggtca cataagccag tttagaaccg tctggtgacc acgccggaga
    42121 catcagcggc tgcggtgaac ggtgaacgac aaactggttg taaccgtcat agtcagatac
    42181 gcgcagttca tacgggaact gaccgccgtt ggtctgaaca acgtaggcaa tacgagtacg
    42241 gaacgcacct ttaatgccgg tcagtttttc aaacacttca tcactggcgg tatgaccagc
    42301 ataacgcagc cactgcttgt tcactttgta cgagttctga gcaagtacag tacccggtgc
    42361 gccgccagtg tcaacaagtt gataagcaac attgtaagaa ccatccggat tcggagtgac
    42421 ctgaccgaca actacagcgt caatgcccag tgcggaccat gcagctggtt gtacttcctg
    42481 cgcactaccc ggctgctgtg gcagacgagc gcgatctaac ggattaaatt taccgctgtt
    42541 acgcaagtca gcagcaacga tgccgccaat atcttcaggt gccgcaccag gccccgccca
    42601 ctggaaagga acaacaccaa taggacgacc ggaatctaca ccgctgtcga tcacaatgcg
    42661 gacttcagca tgcagaactg atgcccacag tatgagaaaa ccaaatgcta ctcgtaatgc
    42721 ctgcttcatc atatctccct tatctggacc gatggcccac gataatttag cagaatgtta
    42781 acaaactaaa atacacaaaa ctaccagaac cccgtggcaa ccggtgcctg atgttgaccg
    42841 tccgaacagt caacatcgcg attacggttt gaagtccaat ggcgcgtttt tgaacacttc
    42901 atatactgcc tggcttggtg gtttcgggat cttcgcaagt ttagctgctg ccaacgcagc
    42961 ctgacaaagt gcgggatcgc caccttcagg tttgatatcc agtaacatac catcgggtgc
    43021 cagttttatg cgcagcgtac aggttttgcc tgcataggac gatgcgtcat agaacttact
    43081 ttcgatagca gatttaatct gcccggcata gttattgata tcggcccctg atgcgccatt
    43141 gtttttagta ttaccactcc cggcaggcga agcattgttc cctttcgccc ctccccccgt
    43201 tttcggtgca ttcttaccag agcttagctc accgaaaata tcatctgcct ctgcggccgc
    43261 ttttgctgca gcggcttttt cagctgcagc ttttgccgct gccgcttttt tgtctgcggc
    43321 ggctttttct gccgctgctt tcttatcagc tgctgccttt tcagcagccg ctttttctgc
    43381 tgcttttttg tcggctgcag ctttctctgc tgccgctttc ttatcagctg cagccttttc
    43441 agcagccgct ttcttctcag cttctgcttt ggctttttca gcagcttcag ttgccgcttt
    43501 ctttcttgct tcagctgcag ctgcttctgc cgcttccgct ttcttcttca gtgccgcagc
    43561 ggctgcctcg gcttttttct gcgcttcggc tgcggctttg gcggcttctg cttctgcttt
    43621 tttctttgcg tctgcagccg ctttcttcgc tgcttcttcc gcagctttag catctgcttc
    43681 ggccttcgct ttagcatctg ccgccgcttt cgctgccgcc tcttcagctt gcttctgctt
    43741 taactcggcc tgttttgcgg cttcttcagc ctgctttttc tgctcctgag ccgctaaccg
    43801 ctctttctca agttgcttca ggcgttcctg ttcagccgct tgtttctcac gcagttcttc
    43861 agcagcctgc tgttccttca tcttgcgctg ttcatcagaa cgcttcgcgc ttgattcctg
    43921 gctttgcatg cgtttgtact gctcaactac cgcacctgaa tcaaccatga cagcgtcgat
    43981 ggacgaacca ccgccgcctc cggctgaagc ttctatattc tcatcgaacg aactccagat
    44041 cagcgccgca aataagatga catgcagcac tgctgaaata attatcgccc gcttgagctt
    44101 gtcgttttgt tcggttgcct ttgacactct cggtttccaa aaactgttcg cctgttaccc
    44161 gctctctttc aagcaaggga aacgcagatg tttagatagg ctgcgtcatt aaaccaaccg
    44221 atttcacacc cgcactatgt aacaagttca gtgctttaat tatttcatcg taaggcacat
    44281 cttttgcgcc accgatcaga aagaccgttt tcgggttggc cttgaaacgg ctggacactt
    44341 ccgccaccac ctgctctggt ggtaaacgct ccaggcgatc tttctcaacc accacggtgt
    44401 actgaccaat accagacact tcaacaatca ctggcggatt atcgttactg ctcaccgcct
    44461 gtgattcagt agcgtctggc agatcgacct ccacgctctg ggtgatgatg ggcgctgtcg
    44521 ccataaagat cagcaacagc accagcagta cgtccagcaa cggtacaatg ttgatttcgg
    44581 acttgagatc gcgacgacct cgtccacgcg ctctggccat ggcttacccc ttgttgctct
    44641 cgctaacggt aaacgcctgg cggtgcagaa tcgcggtaaa ctcttccata aagttgtcgt
    44701 aattcagttc cagtttgttt acgcgctggt tgaggcggtt gtaggccata actgccggga
    44761 tagcggcaaa cagaccaatt gcagtcgcaa tcaacgcttc tgcgataccg ggcgcaacca
    44821 tttgcagtgt tgcttgtttt accgccccga gggcgataaa ggcgtgcatg atcccccaga
    44881 ccgtaccaaa cagaccaata tacgggctga tggagccaac cgtaccgagg aacggaatgt
    44941 gcgtttccag attttcaagt tcacggttca tggagatacg catagcacgc gacgcccctt
    45001 ccactacggc ttccggcgca tggctattgg cacgatgcag gcgcacaaac tctttgaacc
    45061 cgctgtaaaa gatttgttcc gaaccagtca gattatcccg tttcccctgg ctctcttgat
    45121 agaggcgaga gagttcgatt ccagaccaga atttatcttc aaacgcttcg gcttcgcgcg
    45181 ccgctgcgtt aagaatacgg gtccgctgga taataatggc ccaagatgcg attgaaaaac
    45241 caatcaaaat caacatgata agtttaacca gaaggctagc cttcaggaac aaatcaagga
    45301 tattcatgtc agtcactgct taaactccgc gacaatagac ttgggaagcg cacgaggctt
    45361 cattttgagt gggtcaacgc aaacaaccag aacctctgct tcattcagca aagtattctc
    45421 ggcgttgaca atacgttgcg tgaaaaccaa agaggtgcca cgcattgatg ttatttcagt
    45481 ctgtatttcg agcatatcgt cgagccgcgc aggtgcgtaa tattccaccg tcattttacg
    45541 taccacaaag gcaacgcgtt cagccatcag cgcctgctga ctgaagtgat gatgacgcag
    45601 catctctgtg cgtgctcttt cataaaaagc gacgtaactg gcgtggtaca ccacaccacc
    45661 ggcatcggta tcttcatagt agacgcgaac cggccatcga aacagcgttg tattcacttt
    45721 acatcccggt aatgcaacaa aagttagagc ttttaaacgc tgctactata cgcgcgcgat
    45781 tgaggtttgg gaatgagggc aagttaaggg agcgtaaaaa tttatgggcc tggagcggcc
    45841 cataattttt taaagagaat tctgatttaa aagaagaaga aaatcagccc gacaatgaga
    45901 acaatatcgg caagcaatgg gcaaaaaatc ccttgccaaa gaaccttctg cggacgaaag
    45961 cccacgccgt gaatcacacc agcacagacg gcccacatca gcaataaacc atgccagatt
    46021 tccagttcac tggtcttcgc ggcaaaacga gatgggtccc aaaacataca tcctgctaac
    46081 agaagcgcca tcacgaagga aagcgcccgc agggggcgct tgtccattac cgcatacaaa
    46141 gtcgcgataa tcttactcat cagatgtctt cttgaccggc tttgcctgat tcgacgtgtt
    46201 caagcgccag cgcggtgatt accccaaacg aacaggcaag aagcgttccc agaatccatg
    46261 cgaaatacca catttttagc tccttactta gtacagagag tgggtgttac gttcaatatc
    46321 ttctttggtg atacgaccga acattttcca gtaacaccag gcggtgtaga gcagaatgat
    46381 cggtaccaga accaccgcaa cccaggtcat gacgttaagc gtcagctggc tggaagttgc
    46441 atcccacatt gtcagacttg cgttcatcat ggtgctggac ggcatcacaa acgggaacat
    46501 tgcgataccg gctgtcagga tgatgcaggc cagcgtcagg gaggagaaca caaacgccca
    46561 cgcggcttta tccatacgtg cagtcaggat ggtcagcagc ggcagaacca cacccagtgc
    46621 cggaatagcc cacagaattg gcgtgttgtt gaagttaacc agccatgcgc caacttcacg
    46681 aaccacttct ttattcagtg ggttagaggc tgcgtaatgg tccattgtcg atttcacgac
    46741 ataaccatcg ataccgtaca tcacccatac gccagccagt gcgaaacaga ccagtgtcac
    46801 cagcgcagcc acctgagccg ttgcacgggt acgcaggtgc agttcgccca cggtacgcat
    46861 ttgcagatag gttgcgccct gagtaatgat catccctacg ctcaccacgc ctgccagcag
    46921 gccgaacggg ttaagcaact ggaagaagtt accggtgtag tacagacgca gatattcatc
    46981 aacgttgaac ggtacgccct gcaacaggtt accgaacgct acaccaatta ccagcggcgg
    47041 aacgaagcta ccaatgaaga tgccccagtc ccacatgtta cgccagcggg tttcttcaat
    47101 cttggagcgg tagtcaaaac cgaccggacg gaagaacaaa gacgccagca cgaggatcat
    47161 cgccacatag aagccggaga acgcagcggc atagaccatc ggccaggcag caaagagtgc
    47221 gccgcccgcg gtgatcagcc aaacctggtt accgtcccag tgtggtgcaa tggagttaat
    47281 cataattcga cgctcggtgt cgttacgacc gaggaaacgg gtgagcatgc ccacccccat
    47341 gtcgaaaccg tcagtgactg caaaaccaat cagcagaacg ccaaccagca gccaccagat
    47401 aaaacgcaat acttcataat cgatcatttg acgactcctg tcttagcgtg ccggctgagt
    47461 agtcgtggaa gactgctcaa agtgatagcg accggttttc aggctgcttg ggccgaggcg
    47521 tgcaaacttg aacattaaga acaattctgc caccaggaac agggtataca ggccgcaaat
    47581 cagcaccatt gagaagatga gatcgcctgc ggtcagtgac gagttcgcca cagctgtcgg
    47641 cagcacttca ccgatagccc acggttggcg gccatattca gccacgaacc agcccgcttc
    47701 tacagcaatc cacggcagcg gaataccgta cagcgcggcg cgcagaagcc attttttctc
    47761 gccaatgcgg ttgcggatga cactccagaa agagagcgcg atgattgcca gaagcaggaa
    47821 gccacacgcc accatgatac ggaacgcaaa gtacagcggc gctacacgcg ggatggagtc
    47881 tttggttgcc tgttgaatct gcgcttcagt cgcatcagcc acgtttggcg tatagcgttt
    47941 cagcagcaga ccgtaaccga ggtctttctt catgctattg aactggtcac gaaccgcctg
    48001 gtcggtagaa ccagaacgca gttgttcgag cagagagtac gccttcatcc cgttacgaat
    48061 gcgttcttca tgctgcacca tcagctcttt caggccgata accggggtat ccacggaacg
    48121 cgttgcaatg atgcccagtg cgtaagggat ctgaatcgca aatttgttcg tctcctcttc
    48181 ctgatcagga atgccgaaca gagtaaaggc agcaggcgca ggttgcgttt cccactcggc
    48241 ttcaatagca gccagtttgg ttttctgcac gtcgcccatt tcgtagccgg attcatcacc
    48301 cagaacaata acagacagaa cagcagccat accgaagctg gcagcgatag caaaggagcg
    48361 tttagcgaag gcgaagtcac gacctttcag catataccat gcgctgatac cgaggatgaa
    48421 catcgcgcca gtcacataac cagacgctac agtgtgaacg aatttcacct gagcaaccgg
    48481 gttaagcacc agctcggaga agctcaccat ctccatacgc atagtttcaa agttgaaatc
    48541 ggacgcgatt gggttttgca tccagccgtt cgcaaccaga atccacagtg cggacaggtt
    48601 tgaaccgagc gccaccagcc aggtgacaca catatgctga actttaccca gacgatccca
    48661 accgaagaag aacagaccta caaaggtgga ttcgaggaag aaggccatca gaccttcgat
    48721 tgccagcggc gcaccgaaga tatcccctac atagtgggaa tagtaagacc agttagtccc
    48781 gaactggaac tccatggtca gaccggtagc cacacccaga gcgaagttga taccaaacaa
    48841 cttgccccag aacttggtca tatctttata aatctgtttg ccggagagga cgtagaccgt
    48901 ttccataatg gccagcagga acgccatacc gagcgtcagt ggcacaaaaa ggaagtggta
    48961 catcgcggtc aaggcaaact gtaagcgcga cagttcgact atatctaaca tcatgactcc
    49021 ttgctcatcg catgaagact ccgagagtga accccgttag aaagggtcac acgcatgccc
    49081 caatagaaat ttgtttgaac actttcatca taccatgtga atgcacacaa aaacggcgta
    49141 aagaaaggtt gcaaacatgt taataaaaac tcaaattgat cccacgtata tattacgccg
    49201 caaaatcctt acaataaaca ggtttttgtt gaagcaaatt tgcatttttc gacggtgatc
    49261 aatttatagc taaattaccg cctttcagcc aatttgatcg agaacaattt atctcttttt
    49321 gatgcccatt tccaagactt atacattgat aaatatcaat tcctacaaat tatgttaatc
    49381 atgtttcaat aaggtaactt tagtgacatt tatgtttaaa atgtgtgagt tataaggctg
    49441 aaattagttt cggttggcaa ttaaagataa aaagagacga agcaaaaaca gcacggttga
    49501 c
//
LOCUS       DAPHBJ010000009        48185 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.009, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000009 DAPHBJ010000000
VERSION     DAPHBJ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.009"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            163..1380
                     /gene="alaA"
                     /locus_tag="RHZ76_000507"
     CDS             163..1380
                     /gene="alaA"
                     /locus_tag="RHZ76_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HDV2811202.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1464..2063
                     /gene="yfbR"
                     /locus_tag="RHZ76_000508"
     CDS             1464..2063
                     /gene="yfbR"
                     /locus_tag="RHZ76_000508"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HDV2811203.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2122..3954)
                     /locus_tag="RHZ76_000509"
     CDS             complement(2122..3954)
                     /locus_tag="RHZ76_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDV2811204.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4041..4691)
                     /gene="hxpA"
                     /locus_tag="RHZ76_000510"
     CDS             complement(4041..4691)
                     /gene="hxpA"
                     /locus_tag="RHZ76_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HDV2811205.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLSHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4702..5196)
                     /locus_tag="RHZ76_000511"
     CDS             complement(4702..5196)
                     /locus_tag="RHZ76_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HDV2811206.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5279..5734)
                     /locus_tag="RHZ76_000512"
     CDS             complement(5279..5734)
                     /locus_tag="RHZ76_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HDV2811207.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6073..7275
                     /gene="ackA"
                     /locus_tag="RHZ76_000513"
     CDS             6073..7275
                     /gene="ackA"
                     /locus_tag="RHZ76_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDV2811208.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7350..9494
                     /gene="pta"
                     /locus_tag="RHZ76_000514"
     CDS             7350..9494
                     /gene="pta"
                     /locus_tag="RHZ76_000514"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDV2811209.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9684..11204
                     /gene="yfcC"
                     /locus_tag="RHZ76_000515"
     CDS             9684..11204
                     /gene="yfcC"
                     /locus_tag="RHZ76_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDV2811210.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLLGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11237..11779)
                     /gene="yfcD"
                     /locus_tag="RHZ76_000516"
     CDS             complement(11237..11779)
                     /gene="yfcD"
                     /locus_tag="RHZ76_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HDV2811211.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11837..12388)
                     /gene="yfcE"
                     /locus_tag="RHZ76_000517"
     CDS             complement(11837..12388)
                     /gene="yfcE"
                     /locus_tag="RHZ76_000517"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDV2811212.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12444..13088)
                     /gene="yfcF"
                     /locus_tag="RHZ76_000518"
     CDS             complement(12444..13088)
                     /gene="yfcF"
                     /locus_tag="RHZ76_000518"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HDV2811213.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13224..13871
                     /gene="yfcG"
                     /locus_tag="RHZ76_000519"
                     /pseudo
     CDS             13224..13871
                     /gene="yfcG"
                     /locus_tag="RHZ76_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
     gene            13928..14290
                     /gene="folX"
                     /locus_tag="RHZ76_000520"
     CDS             13928..14290
                     /gene="folX"
                     /locus_tag="RHZ76_000520"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HDV2811214.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14311..15204
                     /locus_tag="RHZ76_000521"
     CDS             14311..15204
                     /locus_tag="RHZ76_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDV2811215.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15252..16142)
                     /gene="rpnB"
                     /locus_tag="RHZ76_000522"
     CDS             complement(15252..16142)
                     /gene="rpnB"
                     /locus_tag="RHZ76_000522"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HDV2811216.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16339..17112)
                     /gene="hisP"
                     /locus_tag="RHZ76_000523"
     CDS             complement(16339..17112)
                     /gene="hisP"
                     /locus_tag="RHZ76_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HDV2811217.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQRFLKGSLK"
     gene            complement(17120..17836)
                     /locus_tag="RHZ76_000524"
     CDS             complement(17120..17836)
                     /locus_tag="RHZ76_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2811218.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17833..18519)
                     /gene="hisQ"
                     /locus_tag="RHZ76_000525"
     CDS             complement(17833..18519)
                     /gene="hisQ"
                     /locus_tag="RHZ76_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HDV2811219.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18609..19391)
                     /gene="hisJ"
                     /locus_tag="RHZ76_000526"
     CDS             complement(18609..19391)
                     /gene="hisJ"
                     /locus_tag="RHZ76_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HDV2811220.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19612..20394)
                     /gene="argT"
                     /locus_tag="RHZ76_000527"
     CDS             complement(19612..20394)
                     /gene="argT"
                     /locus_tag="RHZ76_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HDV2811221.1"
                     /translation="MKKSILALSLLVGLSTAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20660..21229)
                     /gene="ubiX"
                     /locus_tag="RHZ76_000528"
     CDS             complement(20660..21229)
                     /gene="ubiX"
                     /locus_tag="RHZ76_000528"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HDV2811222.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21324..22841)
                     /gene="purF"
                     /locus_tag="RHZ76_000529"
     CDS             complement(21324..22841)
                     /gene="purF"
                     /locus_tag="RHZ76_000529"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDV2811223.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22878..23366)
                     /gene="cvpA"
                     /locus_tag="RHZ76_000530"
     CDS             complement(22878..23366)
                     /gene="cvpA"
                     /locus_tag="RHZ76_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HDV2811224.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23625..24287)
                     /gene="dedD"
                     /locus_tag="RHZ76_000531"
     CDS             complement(23625..24287)
                     /gene="dedD"
                     /locus_tag="RHZ76_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HDV2811225.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24277..25545)
                     /gene="folC"
                     /locus_tag="RHZ76_000532"
     CDS             complement(24277..25545)
                     /gene="folC"
                     /locus_tag="RHZ76_000532"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDV2811226.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSLVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25615..26529)
                     /gene="accD"
                     /locus_tag="RHZ76_000533"
     CDS             complement(25615..26529)
                     /gene="accD"
                     /locus_tag="RHZ76_000533"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HDV2811227.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26685..27344)
                     /locus_tag="RHZ76_000534"
     CDS             complement(26685..27344)
                     /locus_tag="RHZ76_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV2811228.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27427..28239)
                     /gene="truA"
                     /locus_tag="RHZ76_000535"
     CDS             complement(27427..28239)
                     /gene="truA"
                     /locus_tag="RHZ76_000535"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDV2811229.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTALRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28239..29252)
                     /locus_tag="RHZ76_000536"
     CDS             complement(28239..29252)
                     /locus_tag="RHZ76_000536"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDV2811230.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFVQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29318..30454)
                     /gene="pdxB"
                     /locus_tag="RHZ76_000537"
     CDS             complement(29318..30454)
                     /gene="pdxB"
                     /locus_tag="RHZ76_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HDV2811231.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLYDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30553..31548
                     /gene="flk"
                     /locus_tag="RHZ76_000538"
     CDS             30553..31548
                     /gene="flk"
                     /locus_tag="RHZ76_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HDV2811232.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGTGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRVMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31545..32616)
                     /locus_tag="RHZ76_000539"
                     /pseudo
     CDS             complement(31545..32616)
                     /locus_tag="RHZ76_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(32881..34101)
                     /gene="fabB"
                     /locus_tag="RHZ76_000540"
     CDS             complement(32881..34101)
                     /gene="fabB"
                     /locus_tag="RHZ76_000540"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HDV2811233.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34260..36266
                     /gene="mnmC"
                     /locus_tag="RHZ76_000541"
     CDS             34260..36266
                     /gene="mnmC"
                     /locus_tag="RHZ76_000541"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HDV2811234.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQARLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAISAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36387..36665)
                     /locus_tag="RHZ76_000542"
     CDS             complement(36387..36665)
                     /locus_tag="RHZ76_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HDV2811235.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(36699..37247)
                     /gene="epmC"
                     /locus_tag="RHZ76_000543"
     CDS             complement(36699..37247)
                     /gene="epmC"
                     /locus_tag="RHZ76_000543"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HDV2811236.1"
                     /translation="MNSTHHYEQLIEIFNSCFADDFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37247..38056)
                     /locus_tag="RHZ76_000544"
     CDS             complement(37247..38056)
                     /locus_tag="RHZ76_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDV2811237.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38056..38880)
                     /gene="mepA"
                     /locus_tag="RHZ76_000545"
     CDS             complement(38056..38880)
                     /gene="mepA"
                     /locus_tag="RHZ76_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HDV2811238.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCFDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(38884..39969)
                     /gene="aroC"
                     /locus_tag="RHZ76_000546"
     CDS             complement(38884..39969)
                     /gene="aroC"
                     /locus_tag="RHZ76_000546"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDV2811239.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40004..40936)
                     /gene="prmB"
                     /locus_tag="RHZ76_000547"
     CDS             complement(40004..40936)
                     /gene="prmB"
                     /locus_tag="RHZ76_000547"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDV2811240.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            41102..41653
                     /gene="smrB"
                     /locus_tag="RHZ76_000548"
     CDS             41102..41653
                     /gene="smrB"
                     /locus_tag="RHZ76_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HDV2811241.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(41725..42582)
                     /locus_tag="RHZ76_000549"
     CDS             complement(41725..42582)
                     /locus_tag="RHZ76_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HDV2811242.1"
                     /translation="MKILRYVFYFLLLICLSTGVQAAKKIATLYIPAGSMGVLTGFQF
                     NLSILTPEGAQYGVYQSRSVAILNVPLVSWTGQGTAPELHLEDARTILPTSRCPGLEE
                     YDARTPQTTWACVEMKVTVFYDGDMHGCPWIVSSYVKSEDATEAIYTGNNVPYIGPTK
                     VSSDCSAIPLDPYDVSWNENTVVHNKIVRIQSTGAVIEQTLPTYLMEGGKLCDGSKYD
                     ERGAYCRFVAQQMTFSTSGCDDAKVTVTPEPQPITSRQLHDMKLRVDTTAQQPIDATC
                     RFTYILNMY"
     gene            complement(42584..43123)
                     /locus_tag="RHZ76_000550"
     CDS             complement(42584..43123)
                     /locus_tag="RHZ76_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2811243.1"
                     /translation="MKTAHIYSPGYALLAGLLLTASTGARAVDNNLHFYGNLLSKSCT
                     LVVDGANLAEIHFPTVSNRDLIVAGQSVHLPVVFKLKDCKGPAGYNVKVTLSGTEDSG
                     QPGFLAVDDTSTAQGVGIGMETLDGKRVDINNTTGATFALTNGNNDINFRARIQAKSG
                     RDVTIGEFTASLTATFEYI"
     gene            complement(43120..43608)
                     /locus_tag="RHZ76_000551"
     CDS             complement(43120..43608)
                     /locus_tag="RHZ76_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2811244.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQEVDYELSCESDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTTFSAYATLRVD
                     YQ"
     gene            complement(43605..44114)
                     /locus_tag="RHZ76_000552"
     CDS             complement(43605..44114)
                     /locus_tag="RHZ76_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2811245.1"
                     /translation="MSHKFLAGAGLLVLAGWGVSGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVEFGNVVINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            complement(44130..44882)
                     /locus_tag="RHZ76_000553"
     CDS             complement(44130..44882)
                     /locus_tag="RHZ76_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV2811246.1"
                     /translation="MFNLTNTAKIVVPALALLATAVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFKCTGNNCQVDEEQSRKG"
     gene            complement(44902..47547)
                     /gene="yfcU"
                     /locus_tag="RHZ76_000554"
     CDS             complement(44902..47547)
                     /gene="yfcU"
                     /locus_tag="RHZ76_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HDV2811247.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTWPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVG
                     GSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDS
                     VSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSG
                     AEASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYG
                     KGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSEMVRTGNDKE
                     MYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNMSVSLTGY
                     RYEYDNRADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVG
                     TSDKHTSVDGYYSHDGSLAQVDLCANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEA
                     TQSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDD
                     DGNVYLAGVKPGEHMRVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVTSPA
                     VKPAIQEQTQRVTPTEPPTSISVNQ"
     gene            complement(47630..>48185)
                     /locus_tag="RHZ76_000555"
     CDS             complement(47630..>48185)
                     /locus_tag="RHZ76_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV2811248.1"
                     /translation="FVKTAIAAAMVMGAFTSTATIAAGNNGTARFYGTIEDSVCSIIP
                     DDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSTNSGN
                     YYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTATNKGKAKQTLNFKAW
                     LVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        11696 a        13060 c        12093 g        11336 t
ORIGIN      
        1 cattattttg tcattattaa gttattaaca gcacaatcga gccttcccct ctggcaaaat
       61 cttattctgc agaccttcaa aacaccgtcc tgggggagta cattgttcta agctgacttc
      121 cacggcaggg agtggcgata acagcaaaaa aggtcaagat tcatgtcccc cattgaaaaa
      181 tccagcaaat tagagaatgt ctgttatgac atccgtggtc cggtgctgaa agaagcaaaa
      241 cgcctggaag aagaaggtaa caaggtactg aaactgaaca tcggcaaccc agccccgttc
      301 ggttttgacg cgccagatga aatcctcgtt gacgtgatac gcaacctgcc taccgctcaa
      361 gggtattgcg attccaaagg tctttactcc gcgcgtaaag ccatcatgca gcactaccag
      421 gctcgtggca tgcgtgatgt taccgtggaa gatatttaca tcggcaatgg tgtatcggag
      481 cttatcgttc aggcaatgca ggcattgctg aacagcgggg acgaaatgtt ggttcctgca
      541 ccagattacc cactatggac cgcggcggtt tcgctttcca gcggtaaagc ggtgcattat
      601 ctttgcgatg aatcctctga ctggttcccg gacctcgatg atattcgcgc taaaattacg
      661 cctcgtacgc gtgggatcgt tattatcaac ccaaataacc caaccggcgc ggtatattcc
      721 aaagagcttt taatggagat tgtggagatt gcacgtcagc ataatctcat tatcttcgcc
      781 gatgaaattt atgacaaaat tctctacgac gacgctgagc atcactcaat tgcaccgctg
      841 gcacctgacc tgctgaccat tacctttaac ggactgtcga aaacgtaccg cgttgcaggc
      901 ttccgtcagg ggtggatggt gttgaacggg ccgaaaaaac acgccaaagg ctacatcgaa
      961 ggtctggaaa tgctggcttc aatgcgcctg tgtgctaacg ttcctgcgca acacgccatt
     1021 cagaccgcgc taggtggtta tcagagcatc agtgaattta ttacccctgg cggtcgtctt
     1081 tatgagcagc gtaaccgcgc gtgggaactg atcaacgata ttccgggcgt ttcctgcgtg
     1141 aaacctcgtg gtgcgctgta tatgttcccg aaaatcgacg ccaaacgctt taacattcac
     1201 gacgatcaga aaatggtgct ggatttcctg ttgcaggaaa aagttctgtt ggtgcaaggg
     1261 acggcattca actggccgtg gccggatcac ttccgcattg tcacgctacc gcgtgtcgat
     1321 gatatcgagc tgtctttgag caagttcgcg cgtttccttt ctggttatca tcagctgtaa
     1381 tcttaatttc actgccggag attgcatccg gcagcgttat cccgccacaa tgacctgatg
     1441 atgtcatcat acgtaaggtc actatgaaac agagccattt ttttgcccat ctctcccgcc
     1501 tgaaactcat taaccgctgg ccgctcatgc gcaacgtgcg gacggaaaat gtgtccgaac
     1561 acagtttgca ggtagcgatg gtcgcccatg cgctggcagc tatcaaaaat cgaaaatttg
     1621 gcggtaatgt caacgccgaa cgtatcgctt tactggcgat gtaccacgat gcctcagaag
     1681 tgctcaccgg cgatctccct actccggtga aatacttcaa ttcgcaaatc gctcaggagt
     1741 acaaggctat tgaaaaaatc gctcagcaaa aactggtcga tatggttccg gaagagctgc
     1801 gggatatctt tgcgccgtta attgacgagc atgcatatag cgatgaagaa aaatcgctgg
     1861 tgaaacaggc agatgcactg tgtgcatatc tgaaatgtct ggaagaactc gcggccggaa
     1921 ataatgaatt cttgctggca aaaacgcgac tggaagcgac gcttgaagcg cgtcgcagcc
     1981 aggagatgga ctacttcatg gaagtatttg ttcccagctt ccatctttcg ctcgatgaga
     2041 ttagccagga ttcaccgctg taagcagccg gagtctgcgt cgcatcaggc aataagcgcc
     2101 ggatgcgaca tcaggctctt gtcaaaacgg aaacagcatc gggatcatca ccacacaaac
     2161 cgccatcacg ataatggtga acggtacccc caacttcaca aagtcactga agctgtaatt
     2221 tcccggacct aaaaccagtg tgttaacagg tgaagaaacc ggtgtcataa aggcggcgga
     2281 tgctgccatc gccacgacca tcgcgaatgg ataaggcgac acccccatcg ttttggcagc
     2341 agccagcgca atcggagcca tcaacaccgc cgtcgcggta ttagagataa atagcccaat
     2401 aaccgccgac aagacaaaca aacaccccag catcatatgt ggcccgtaac caccgccaat
     2461 gtccatcagc cctttcaccg ccagcgcgac acctcccgtt ttctgtaatg ccacagcaaa
     2521 cggcatcatc ccaacgatca aaataatgct cggccagtga atggatttat aggcgctttc
     2581 agcatctata cagcggaatt tccccatcag caggcaggcg atgatagcgg caacaggatt
     2641 aggaatttca tcggtcagca ttaacgccac cattagcacc agacagaaaa tggcatgggg
     2701 tgcctggctg tgcgcgggcg atgcttcact cacctcttcc ggtaagttca gcgctacgaa
     2761 gtcgcggccc tgtttggcca gcataccgat cagtttccag ttacccacaa ccaggatgat
     2821 atcgcccagc agcagaggct catccgccag cgaaccttcc agcgccacgc cattgcgctt
     2881 tagccccacc acattcagtc cgtagcgggt acgaaaacca atttcgcgca ccgatttacc
     2941 aatcagttct gactcaggaa ttaatgaaat ctctgccatg cccacatcaa gggcctggtc
     3001 agaaaaatac tcgccgcgca gtaccatcgg ctccagcaat tgctcactac aaaattgccg
     3061 gagatcgaca tcagccgcag acatatcaat aagcaaaacg tcacgcgcgc gaaattcaga
     3121 aaccccatta acgttcacga taacgcgacg aaaacgccgc cagcgttcaa caccgatgac
     3181 gttagcgcca taacgctcac gtaatttgag atcatccagc cgttgaccaa tcattggcga
     3241 tccggggcga atagccagac gtcgcgcacg cccggtcagt cgatattcac ggataagatc
     3301 gcgaaaggtt cgacgcgtcc agccttcgcg ctgcggggtc tgggtatccc ctttcagcat
     3361 gaaacgcatc actaacatat acaaaatacc cagcaccagc acgaccaggc caataggtgt
     3421 tacgctaaag aaactgaagc cgtgatagcc ttcacgcagc aattcactgt tgactaccag
     3481 gttcggcggc gtcgccacca gcgtcatcat gccgctaatc agcccggcaa aacttaacgg
     3541 catcatcaga cgtgacggcg aagtttgcat acgcatggca acgcttaaca ccacgggaat
     3601 aaagatagcg acaacgccgg ttgaactcat aaacgcgcca agccccgcga cggtcagcat
     3661 caacaaaacc aacattttga tttcactgtt gcccgccact ttgaccagcc atgttcccat
     3721 tacggtggca acaccggtac ggaccaaacc atcgccaata ataaacaagg cggcaatcag
     3781 gacaacgtta ggatcagaaa agccggaaaa tacttcgggg accgtcagcg ttccgcttaa
     3841 tgcaaacgcg acaataacaa acaaagcgac cgcatccata cgcactctgc ccgtcgcaaa
     3901 caagacaatg gcaacggcca gtaatgaaag aacccaaatc aattcaccgt tcacaactta
     3961 tccttgttaa ttgagggaga tgacttgatt ctgccataaa aaagccccga cgagacgggg
     4021 ctaaatcatg atcaggtgtt tcactgaata ataacatcgc catttggctg tttggtcaca
     4081 gtaatttgct ccagactgtg gaggaccaaa tcgacctcat tcaggcgcgg ggtatctgcc
     4141 ggagcgttaa ccgcaatgac atggcaaccc gccgccaggc cagaaagcac gccagcggga
     4201 gcatcttcca ccaccacaca ctcctgcggc gcaagcccca gcagctgcgc gcctaacaga
     4261 tacgcatcag gttctggttt tccgcgcttc actcgctcag cggttacaaa cacctctggt
     4321 gcgggaagcc cagctatttt atggcgcgct cgcgctaccg gcatggagcc agaagtcaca
     4381 atggcccacg gaatacctgc tttattcaaa tgactgagta aggcgattgc ccccggaagc
     4441 gcggtaatac cttcggtttc cgtggcctcg atgtgctcca gacgcgtaaa ctcggcggca
     4501 atatcagcct cggatttgcc cgccataaaa tggcgcagag aggtgatcgc ctgtttaccg
     4561 tgaatgaaag ccagcacctc ttccggcgct aacccatgac gtctggccca gttgctccac
     4621 gcccgttcta ccgcaggcag ggaatccacc agcgttccat caagatcaaa cagaaaacct
     4681 ttgcaccgca cgcgggcctc ctcaggcatt gataatttga ttaatttcgt tggcgctcaa
     4741 atggtactga cgcgggcagg catgccacac attaagcatg cgctgatatt tttcccacat
     4801 tggcgtctgg gcgttaaaac cgtgagttcc ggcgtcaaaa tgggtatagc gcccttccac
     4861 attaaccata aagcggacat aaccgaggta acgtgcttca gtggcggcgt caaagccgag
     4921 gaaggtgaca cgacgttcat cgatggattg ctggtcctgc aaattagacc aggaaacatg
     4981 caacgcatga tacatctcca taatgtcgat gatagtgcgg caggtttctt ctttcagctc
     5041 gccaaactcg cgatccaatt cacgcatctg taatccgtaa ccacgctcaa taattgtttg
     5101 caggcgacgg taacgttcag catttgccgg atcgagcata gtcatcatct tgtactgatt
     5161 agacaaaata agacgttgcg cgttggtcat ttccattgtt gactcctgta tcactctact
     5221 acggtgaaaa aaaagaaggc tgagtatgcc ttcttttata tgcgtaatca ggggtcaatt
     5281 acaaatcatc aaggaaagtt ttatccagtt gtttgaaggc gcgcttaagc gtgtcagcta
     5341 atgcctggta atcaggcttg ccttcaacgg gtgccaacac ctgtccagac tcctgcaatt
     5401 taccgcgaac ttcataaaac cagttgagga tagcaggggg taatggcgtt acagaacgct
     5461 tgcccagcca ccacaatccc tgcatgggta aacttaaggc gaacagcgca gtggcaactg
     5521 ccggcccaag ctgaccgccc agggcaatct gccagcagag agtaaatacg gcgatcggcg
     5581 gcataaaacg gatcgcataa cgcgtcatct tgataacgcg attttcgaca aagaccgggg
     5641 caaggcgttt ttccagcggc cacgtctttg agtaatgctg tccccggcga aacaagctaa
     5701 aaaaattaac agaacgatta tccggcgttg acatgcttca cctcaacttc acatataaag
     5761 attcaaaaat ttgtgcaaat tcacaactca gcgggacaac gttcaaaaca ttttgtcttc
     5821 catacccact atcaggtatc ctttagcagc ctgaaggcct aagtagtaca tattcattga
     5881 gtcgtcaaat tcatatacat tatgccattg gctgaaaatt acgcaaaatg gcatagactc
     5941 aagatatttc ttccatcatg caaaaaaaaa tttgcagtgc atgatgttaa tcataaatgt
     6001 cggtgtcatc atgcgctacg ctctatggct ccctgacgtt tttttagcca cgtatcaatt
     6061 ataggtactt ccatgtcgag taagttagta ctggttctga actgcggtag ttcttcactg
     6121 aaatttgcca tcatcgatgc agtaaatggt gaagagtacc tttctggttt agccgaatgt
     6181 ttccacctgc ctgaagcacg tatcaaatgg aaaatggacg gcaataaaca ggaagcggct
     6241 ttaggtgcag gcgccgctca cagcgaagcg ctcaacttta tcgttaatac tattctggca
     6301 caaaaaccag aactgtctgc gcagctgact gctatcggtc accgtatcgt acacggcggc
     6361 gaaaagtata ccagctccgt agtgatcgat gagtctgtta ttcagggcat caaagatgca
     6421 gcttcttttg caccgctgca caacccggct cacctgatcg gtatcgaaga agctctgaaa
     6481 tctttcccac agctgaaaga caaaaacgtt gctgtatttg acaccgcgtt ccaccagact
     6541 atgccggaag agtcttacct ctacgccctg ccttacaacc tgtacaaaga gcacggcatc
     6601 cgtcgttacg gcgcgcacgg caccagccac ttctatgtaa cccaggaagc ggcaaaaatg
     6661 ctgaacaaac cggtagaaga actgaacatc atcacctgcc acctgggcaa cggtggttcc
     6721 gtttctgcta tccgcaacgg taaatgcgtt gacacctcta tgggcctgac cccgctggaa
     6781 ggtctggtca tgggtacccg ttctggtgat atcgatccgg cgatcatctt ccacctgcac
     6841 gacaccctgg gcatgagcgt tgacgcaatc aacaaactgc tgaccaaaga gtctggcctg
     6901 ctgggtctga ccgaagtgac cagcgactgc cgctatgttg aagacaacta cgcgacgaaa
     6961 gaagacgcga agcgcgcaat ggacgtttac tgccaccgcc tggcgaaata catcggtgcc
     7021 tacactgcgc tgatggatgg tcgtctggac gctgttgtat tcactggtgg tatcggtgaa
     7081 aatgccgcga tggttcgtga actgtctctg ggcaaactgg gcgtgctggg ctttgaagtt
     7141 gatcatgaac gcaacctggc tgcacgtttc ggcaaatctg gtttcatcaa caaagaaggt
     7201 acccgtcctg cggtggttat cccaaccaac gaagaactgg ttatcgcgca agacgcgagc
     7261 cgcctgactg cctgatttca caccgccagc tcagctggcg gtgctgtttt gtaacccgcc
     7321 aaatcggcgg taacgaaaga ggataaaccg tgtcccgtat tattatgctg atccctaccg
     7381 gaaccagcgt cggtctgacc agcgtcagcc ttggcgtgat ccgtgcaatg gaacgcaaag
     7441 gcgttcgtct gagcgttttc aaacctatcg ctcagccgcg taccggtggc gatgcgcccg
     7501 atcagactac gactatcgtg cgtgcgaact cttccaccac gacggccgct gaaccgctga
     7561 aaatgagcta cgttgaaggt ctgctttcca gcaatcagaa agatgtgctg atggaagaga
     7621 tcgtcgcaaa ctaccacgct aacaccaaag acgctgaagt cgttctggtt gaaggtctgg
     7681 tcccgacacg taagcaccag tttgcccagt ctctgaacta cgaaatcgct aaaacgctga
     7741 atgcggaaat cgtcttcgtt atgtctcagg gcactgacac cccggaacag ctgaaagagc
     7801 gtatcgaact gacccgcaac agcttcggcg gtgccaaaaa caccaacatc accggcgtta
     7861 tcgttaacaa actgaacgca ccggttgatg aacagggtcg tactcgcccg gatctgtccg
     7921 agattttcga cgactcttcc aaagctaaag taaacaatgt tgatccggcg aagctgcaag
     7981 aatccagccc gctgccggtt ctcggcgctg tgccgtggag ctttgacctg atcgcgactc
     8041 gtgcgatcga tatggctcgc cacctgaatg cgaccatcat caacgaaggc gacatcaata
     8101 ctcgccgcgt taaatccgtc actttctgcg cacgcagcat tccgcacatg ctggagcact
     8161 tccgtgccgg ttctctgctg gtgacttccg cagaccgtcc tgacgtgctg gtggccgctt
     8221 gcctggcagc catgaacggc gtagaaatcg gtgccctgct gctgactggc ggctacgaaa
     8281 tggacgcgcg catttctaaa ctgtgcgaac gtgctttcgc taccggcctg ccggtattta
     8341 tggtgaacac caacacctgg cagacctctc tgagcctgca gagcttcaac ctggaagttc
     8401 cggttgacga tcacgaacgt atcgagaaag ttcaggaata cgttgctaac tacatcaacg
     8461 ctgactggat cgaatctctg actgccactt ctgagcgcag ccgtcgtctg tctccgcctg
     8521 cgttccgtta tcagctgact gaacttgcgc gcaaagcggg caaacgtatc gtactgccgg
     8581 aaggtgacga accgcgtacc gttaaagcag ccgctatctg tgctgaacgt ggtatcgcaa
     8641 cttgcgtact gctgggtaat ccggcagaga tcaaccgtgt tgcagcgtct cagggtgtag
     8701 aactgggtgc agggattgaa atcgttgatc cagaagtggt tcgcgaaagc tatgttggtc
     8761 gtctggtcga actgcgtaag aacaaaggca tgaccgaaac cgttgcccgc gaacagctgg
     8821 aagacaacgt ggtgctcggt acgctgatgc tggaacagga tgaagttgat ggtctggttt
     8881 ccggtgctgt tcacactacc gcaaacacca tccgtccgcc gctgcagctg atcaaaactg
     8941 caccgggcag ctccctggta tcttccgtgt tcttcatgct gctgccggaa caggtttacg
     9001 tttacggtga ctgtgcgatc aacccggatc cgaccgctga acagctggca gaaatcgcga
     9061 ttcagtccgc tgattccgct gcggccttcg gtatcgaacc gcgcgttgct atgctctcct
     9121 actccaccgg tacttctggt gcaggtagcg acgtagaaaa agttcgcgaa gcaactcgtc
     9181 tggcgcagga aaaacgtcct gacctgatga tcgacggtcc gctgcagtac gacgctgcgg
     9241 taatggctga cgttgcgaaa tccaaagcgc cgaactctcc ggttgcaggt cgcgctaccg
     9301 tgttcatctt cccggatctg aacaccggta acaccaccta caaagcggta cagcgttctg
     9361 ccgacctgat ctccatcggg ccgatgctgc agggtatgcg caagccggtt aacgacctgt
     9421 cccgtggcgc actggttgac gatatcgtct acaccatcgc gctgactgcg attcagtctg
     9481 cacagcagca gtaatctcgt catcatccgc agctttgcgc tgcggatatc tgaaccggaa
     9541 ataatcacta tttccggttt tttattctct taatctgcat taatcctttc tgattatctt
     9601 gcttaactgc gctgcatcaa tgaattgcgc catttcactt tgcatactta ccactttgtt
     9661 ttgtgcaagg gaatatttgc gctatgtccg caatcactga atccaaacca acaagaagat
     9721 gggcaatgcc cgatacgttg gtgattatct tttttgttgc tattttaacc agccttgcca
     9781 cctgggtagt tccggtgggg atgtttgaca gtcaggaagt gcagtatcag gttgatggtc
     9841 aaacaaaaac acgcaaagtc gtagatccac actcatttcg cattctgact aacgaagcag
     9901 gcgaacctga gtatcaccgc gtacagctgt tcacgacggg cgatgaacgc ccgggcctga
     9961 tgaacttccc gtttgaaggg ttaacctcag gatcgaaata cgggacagcc gttggcatca
    10021 tcatgtttat gctggtgatt ggcggcgcgt ttggcattgt gatgcgtaca ggaaccattg
    10081 ataacggtat cctggcgctt attcgccata cccgcgggaa tgaaattctc tttattcctg
    10141 cgctgtttat tctgttttca cttggcggcg cggtatttgg tatgggagaa gaggccgtcg
    10201 cctttgccat tatcatcgca ccgctaatgg tccggctggg ctatgacagt attaccaccg
    10261 tcctggtgac ctatattgcc acgcaaatcg gttttgccag ttcgtggatg aacccgtttt
    10321 gtgtggtcgt tgctcagggg attgccggcg ttccggtgct ttctggctcc gggttgcgca
    10381 tcgtggtgtg ggttatcgcc actatgattg gcctgatctt taccatggtg tacgcctcac
    10441 gagtgaaaaa gaatcctctt ctgtcacgcg tgcatgagtc cgaccgcttc tttcgtgaaa
    10501 agcaggcgga tgttgaacaa cgtccgttta cctttggtga ctggctggta ttgattgtcc
    10561 tgaccgccgt aatggtctgg gtgatttggg gcgtgatcgt taatgcctgg tttattccag
    10621 aaattgccag ccagttcttc accatgggtc tggtgattgg catcatcggc gttgttttcc
    10681 gccttaacgg catgacggtt aataccatgg cttcatcctt taccgaaggg gcgcgaatga
    10741 tgatcgcccc tgccctgctg ttgggtttcg ccaaagggat tttgctgctg gtcggtaatg
    10801 gtgaagcggg tgatgccagc gtgttaaata ccatcctcaa cagcattgcc aatgccatta
    10861 gcggtctgga caacgcggtc gcggcctggt ttatgttgct cttccaggcg gtatttaatt
    10921 tcttcgtgac gtccggttct ggtcaggcgg cgttaaccat gccgttactg gcaccgcttg
    10981 gcgatctggt cggtgttaac cgtcaggtta ccgtgctggc attccagttt ggtgatggct
    11041 tcagtcacat catttaccca acctcagctt cgttaatggc aactctcggt gtgtgcaggg
    11101 tggacttccg taactggctg aaggtgggcg cgaccctact tggactgctg tttattatgt
    11161 ccagcgtcgt agtgatcggc gctcagttga tgggctacca ctaaaaatgt taagagccgc
    11221 aattgcggct cttttttcat tctgccgttt cagtctctac ggcttcattt ttggcattgc
    11281 gcttcatcca caacgccagc gctttcagcg agtctggagt gaactcatcg cagcgtgcgg
    11341 tgatttcttc cggcgtcagc cagcaaactt cactgacttc atcttcctgt agggcgaagg
    11401 gaccgtgaga gacgcagctg aacaacgcgc cccagacacg gcaattttta tcttcgaaat
    11461 agaactgccc gtgctcggca aaggggacac cggcaatgcc caactcttct tccgcttcgc
    11521 gacgcgcgga ttccagcagt tgctcatcgg cctggactac accgcctgcg gtcgcatcca
    11581 acatgccggg taaaaagtct tttgtctcgg tacgacgctg gaccagaatt ttgcccatgc
    11641 catcatgcac gacgatgtaa gttgcacgat gacgcagaca ctgtgcccgc atttgttccc
    11701 ggctggcttg tgcaatgact tcgttctctt cattgacaat atccacccat tcagtacttg
    11761 ccaaacgacg ctgttccatt atcaggaaac cttctttttc tggcgctctt acggcgcatt
    11821 ttgagttgtg ggtaaattac ggattaatcg cgacctgcgc aatgatactt tgatcattga
    11881 gtgcgattac actaagtacg tcattatcca gcatgccata actcgccgga ttaccgcctt
    11941 tcggaatact caccgagccg gggttgaagt ggaaaatctc cccccgctgt tctgccactg
    12001 gcagatgggt atgaccgtac accagcacat cgttctggtt taaagcaggt agattttccg
    12061 ggccaaaaag atgaccatgc gtcaaaaaca gacgttgttt ttccagtaat acctgttgcc
    12121 acggcgcggt tatcgggaaa tgcagcagca tttgatccac ttcgctgtcg cagttgccgc
    12181 gcacagcgat aaccttatgt gccacttcat taagccgttc agcgactttg gctggcgcgt
    12241 aaccctccgg taaagcatta cgcgggccgt gattcaacac gtcgccaagg atcaccagcc
    12301 actgggcacc gctttgggca aacaactcca gaacacgttc cgtcgccggt aacgacccat
    12361 gaatgtccga tgcaaacatc agtttcatca ctcactcctc gtcgaaaaaa cgtaagctat
    12421 gatactggat tctgtcgttg ctatcagcca gattgcttcg ccgaaagtgc aataaaacgc
    12481 tggacagacg ctcgctgcca ctggaatgtc gcataatcca ccaggcgttc tggcacctca
    12541 tcgccatgta gtaccaggcg gttaatcatt agcgccagat cagtatcagc aatgcaccat
    12601 tcaccaaata aattcggctg acccagtgct aacaaatgtt ctgccatcgc gaacagtttc
    12661 tctgcactgg cttttccctc ggccgttagt ggcgcttttt tcgcccccgc aaagacaaca
    12721 tccgtcggac gctcttcgcg gatgggcatc agatcgctgc gcagccaggc ctgaatctgt
    12781 cgtgcacgcg cacgattttc taaatcaagc ggataaatac gttcccaggt cggtggcgca
    12841 aatcgatctt ccagatactc cgcaatggca gaagattcac tcaactcaaa atcatcgatt
    12901 tgtaataacg gcacacggcg tgtctgaccg taaccttgcc acgtcggctg caaatgttca
    12961 ccgctgtcga ggtcgatggt cttgatatga aacgacaggc ctttttcctg caacgccacc
    13021 caggcggata acacataagg ggagaaaaag tgggcatctg accaaagcgt gatagcgggt
    13081 ttactcatta tgtcctcggc aataaagggg gctggctatc aaactataga gcctttttgt
    13141 cactgtcacg tgacgaatac tcacgtaagt cgccgcgctt tctatacttg tggtgttcag
    13201 atgattaaac aaccggagct gcaatgatcg atctctattt cgccccgaca cccaatggtc
    13261 acaaaattac gctgtttctc gaagaagcag agctggatta tcgcttgatt taggtagacc
    13321 tggggaaagg cggtcagttt cgtccggaat ttttgcgcat ttcgcccaac aacaaaattc
    13381 cggcaattgt tgatcattct cctgccgatg gcggcgaacc gctaagcctc tttgagtctg
    13441 gtgccatttt gttgtatctg gctgagaaaa caggactctt tttgagtcat gaaacgcgtg
    13501 agcgcgccgc cacattacag tggttattct ggcaggtagg cggactgggg ccgatgcttg
    13561 ggcaaaatca tcattttaat cacgcagccc cccaaaccat tccttacgct attgaacgtt
    13621 atcaggttga aactcagcgt ctttaccatg tactgaacaa gcggctggaa aactcgccct
    13681 ggctgggagg cgagaactac agcattgcgg atattgcctg ctggccgtgg gttaatgcct
    13741 ggactcgcca gcgaattgac ctagcaatgt atccggcagt caagaactgg catgagcgga
    13801 tccgttcgcg ccctgccacc gggcaggcac tgctaaaagc acaactcggt gatgagcgtt
    13861 cggatagtta acagaaacag gttctcgtgt attatttcat cctaagtaaa acaacggaga
    13921 acctgcaatg gcacaacctg ccgctattat tcgtataaag aaccttcgtt tgcgtacgtt
    13981 tatcggaatt aaggaagaag aaattaacaa ccgtcaggat attgttatca atgtgacgat
    14041 ccactacccc gccgataaag cgcgcactag cgaagatatc aacgatgcgc tgaattatcg
    14101 caccgtaacg aaaaacatta ttcagcatgt agagaataac cgtttctctt tgctggaaaa
    14161 attaactcag gatgtgctcg atatcgcacg tgaacatcac tgggtgacgt atgctgaagt
    14221 ggagatcgat aaactgcacg cgctgcgcta cgccgattcg gtatccatga ccttaagctg
    14281 gcagcgttaa tcgccatatc gggaggctgc atgaacatag tgatcaccgg agggacagga
    14341 ttaattggtc gccatttgat tccgcgtttg ctggagctgg gccatcaaat tacggtagtg
    14401 acgcgtaacc cgcagaaagc cagttccgtt ctcggccctc gggtgacact atggcaaggg
    14461 cttgccgatc aaagcaacct caacggcgtt gatgcggtaa tcaacctggc cggagaaccg
    14521 attgctgata aacgctggac tcacgagcaa aaagagcgtc tctgccaaag ccgctggaat
    14581 atcacgcaaa aactggtcga tttgattaat gccagcgaca cgccaccgtc ggtactcatt
    14641 tccggctcgg caacgggcta ttatggtgac ttaggtgaag tggtggttac cgaagaggaa
    14701 ccgccgcata acgaatttac ccataaactc tgcgctcgct gggaagaaat tgcctgccgg
    14761 gcgcaaagtg acaaaacgcg agtgtgcctg ctgcgtaccg gtgtagtgct ggcaccggat
    14821 ggcggtattc tcggtaaaat gctgccgccg tttcgtcttg gcctgggcgg gccgattggc
    14881 tctggtcggc agtatctggc ctggattcat attgatgata tggtcaacgg tattctctgg
    14941 ctgctggata acgagctgcg cgggccattt aatatggttt cgccctaccc ggtacgcaat
    15001 gaacaatttg cccatgcgct cggtcatgcg ctgcatcgcc cggccatttt gcgcgtcccc
    15061 gcgaccgcca ttcgactgtt aatgggcgaa tcttcagtac tggtattagg tggacaacgc
    15121 gcgctgccta aacggctgga agaagcgggt tttgcgtttc gctggtacga tttagaagag
    15181 gcgctggcgg atgtcgttcg ctgatgtggt ttacagcaaa catccgccag ttaactcccg
    15241 gtgttacagg attagtggct ttgcgcgata agatcgtctg gtgaaagtcg ggtcaccatc
    15301 ataactaact ctctgtctaa acctctatcc agcatctcct gagcaatacg cagggcttct
    15361 tcgtgtttgc cctgcattgc gccttcttca cgtaatctgt cagcaatggt catcagtttc
    15421 tccttttctt gtggtgcgcg ttccgctatc tcaccaataa atgcacgaaa acgctgggca
    15481 tcccctgttt gtaatacgta attaaacagg gcttttagct gtctgtcatt agtgttccct
    15541 gtaactagca gcgaaacaat ttggtcgact aatcccaaca gatcgcgctg acgaatatgt
    15601 ttctgaatta actccaacag cgccattttg cggtgttgca taatctcgtc atccggcacc
    15661 acggtaatat ccaccaacgg aaaagccgat gaatatattt tgcgggctat agcaggctcg
    15721 gcaaattcat caagccagca gagtgaataa ggataaggac ttctgcaacc atgataaaac
    15781 agcatcggga tcaccaatgg aagctcttta tagcccgcat caagatggtt ttgcattgcc
    15841 gcaatggaat aacgcatcat gcgaaaagcc attaattctt ccggcttact ttggtgctct
    15901 atcactacat aaatataacc cactccctcc tgcgttttca cagaccacaa gaggtcggaa
    15961 taatattgcc gcaggtcttc atcaataaaa ctgtttggtt ccagtttaag cgtcgttaaa
    16021 tcacacagtt tgcgcagcgg cgcgggaaga tgaatatcaa taaaatcccg cgcggtgtct
    16081 ggatggcgta aaaaagattt aaataccgca tcatgcggcg tggaagttgt cgatattgtc
    16141 atggcgttcc gtcacccctc aaatttaaga tgacgcgaca ataaccacga ttaacaccca
    16201 atgcagcagg cagtttttat ctttggacag cgaattcaga agtattttta gcggactgta
    16261 acttttacat ttcagcggaa gctgactcgc aaattcccca tgaaaatact ccggcagcgt
    16321 tagccaccgg agaggaaatt atttcagcga tcccttaagg aaccgttgca gacgagggct
    16381 ttgcgggttg ccaaataact gctccggcgc gccctcttct tctattttcc cctgatggag
    16441 gaaaatgaca tgagtagaaa catggcgagc aaagcccatt tcgtgagtca ctaccaccat
    16501 ggttttcccc tcttctgcca gttgctgcat aatacgcaac acttcgccta ccagttcagg
    16561 atcgagcgcc gaggtaggtt catcaaacag taaaacttcc ggttccatcg ccagcgcccg
    16621 cgcgatagaa acacgctgtt gctgaccgcc ggaaagatgc accggatatt tcccctgcgc
    16681 acgttcgtct atcccgactt ttgccagata cttcaccgcc cgctcgcgcg cttcctgctt
    16741 gctcaggccc aacacctgaa tcggcgcttc catgacgttt tccagcaccg tcatatggct
    16801 ccagagattg aagtgctgga ataccatcgt caggcgtgtg cgcagtaagc gcagttgatt
    16861 tttatcggcg actttgagtt gaccgtcttt gtcgcgcacc agattgatcg tctggccatt
    16921 gaccacgatc gacccttcac tcggtttttc gaggaagtta atgcagcgca gaaaggtact
    16981 tttccccgat cccgacgatc cgatgatgct tattacatct ccggcattcg cttgcagtga
    17041 tacccctttc agcacttcat gttcgccgta gcgtttgtgc aaatcgataa cgtttaattt
    17101 attctcggac atcgtattct cagtgcgttg aagaaggttt cacatgctgc aaccagcgtt
    17161 tttccgctct gcgaaagagg ctgatcagga cataagagat gattaaatag agcaccgcgg
    17221 caatgccgaa ggcggtaaaa ggttgatacg tggcggcgtt aatatcgcgg gctattttca
    17281 gcagatccgg caccgtggca gtaaatgcca acgcagtaga gtgcagcatc aggatcactt
    17341 cgttgctgta tgccggtaac gcaatacgca gcgcagaagg caaaataatg cagcgataca
    17401 ttttaaaagt cgagaagcca taggctctgg cggcttcaat ttccccatgc ggaaccgaac
    17461 ggattgcccc agcaaaaatc tcggtagtgt aagcgcaggt gttaagcgtc agcgccagca
    17521 cggtacagtt caggccactg cggaagaaag cgttaaggaa ttcggttccc ttaacaatct
    17581 caagcgtgta catgccggaa tagaacacca gcaactgaac atacagcggc gtaccgcgaa
    17641 aaatataggt aaataaccag attggaaact ggatgtattt attactggag acacgaccaa
    17701 tcgccagaaa cagcgccagg actccgccta tcactaccga caaaataagc agccacagag
    17761 tgatcgccac accagtaaag cgataaccgt cggtccacag cagcggtttc cagtattcat
    17821 gtaagatttc gatcacaggt cagccctctt cacacccacg gagtagcggc gctcaaggaa
    17881 cagcagcaca ccattggaaa cagtggtgaa aaccaggtaa atcacgccac agacgatggc
    17941 gaaatagaac ggttcccagg tacttttgcc tgccagttgg gtggctttga ccacatcttc
    18001 caggccgagt aacgaaacca gtgcggtaga tttgaggatc acctgccagt tgttgccaat
    18061 gcctggcagg gcgtaacgca tcatcgccgg aaacatgatc cgccgaaaca cttgcccacg
    18121 agtaaaaccg aacgccgtcg ccgcctctat atgtcctttc ggcactgcca taaaagcacc
    18181 acggaacgtt tcggtaaaat aagcaccgta gataaaaccg agagtgataa taccagcgac
    18241 catcggatcg atatcaatct gcccgacgcc catcgcctcc gtcaccgtgt ttagcgcaat
    18301 ctgcaaaccg tagaaaatca gcagcatcaa cactaagtcc ggcacgccac gaatcagcgt
    18361 ggtgtaccct tcgaaaatca gccccgaaag ccgattttgc gagagcttac cgccagcgcc
    18421 aattaaaccg atgattacag cgagcactac agagctgata gccagctcca gcgtgacgag
    18481 cgcaccctgt aaaataacac ctgaaaaccc atacaacatg ctgcctgtcc tgtcgtgtgt
    18541 ggtgaattac cgcctcttgt ctccctccgg ttacccgaag ggagcgggct ttctttcacc
    18601 gatggcgatt agccaccata aacatcaaaa tcgaagtact ttttcgctaa tttctcgtaa
    18661 gtaccgtcag cgcgcatttc ggcaaaggct ttgttcagtg cttcgcgcag ttcgttatct
    18721 tctttacgca ggcccatgcc ggtccctacg ccaaacagtt tttcatcttt aacagacggg
    18781 ccaccgaatt tgtaatcttt accgacaggt tgtttgagga aaccttcgct ggcagcgacc
    18841 tcatcctgga acgcggcatc aatacgtccg gcagtcaggt cagaataaat gttgtcctgc
    18901 ccctgatacg agacgatttc aatgcctttt ggtgcccaat gttcattacc gaacgtctcc
    18961 tgggtggtgc cctgcaatac gcctacccgt ttgcctttca gcgactcgac tgtcggctga
    19021 atgtcagaat ttttcgccac caccaaacga gaatcggcag cgtacagttt gtcggtgaag
    19081 gctatttctt gctgacgttt ttccgtaatg gaaagcgatg acatgatggc gtcaatcttc
    19141 ttcgctttta aggacgggat taacgcatcc agcggatttt cgacaaacgt acattgcgta
    19201 ttgatgcgtt tgcataattc ctttgccaga tcgatatcga agccaaccag ttcgccttgt
    19261 gaattttttg attcaaatgg cgcataggtc gggtcggtac cgatgcggat gttttgcgga
    19321 atcgcagcaa acgccgcagt tgcgctggag aaggccagaa ccagagagag cgatagcacc
    19381 agttttttca taatgtcctc aactgacagt ctttttatag ggaatattta caggtttgtt
    19441 acacttatcg tgccataaaa ttgaccatca aggcaaaata tcccgccccg ttagacgtat
    19501 tatttttcat taaaaatgct taagtatgca tttcaatgca ccacgatagt gcaacaaacc
    19561 tggcgtgcac catgatgatc attccatcag gtacagcttc ccagcgacgt atcagtcacc
    19621 gtagacatta aagtcgaaat actttttcgc catcttgtcg taggtgccgt cctgacgcag
    19681 ctcgccaagc gccttattga aggcagccgt cagttcagca tcatctttac gtagccctac
    19741 accggtgcca tcaccgaagt attttttgtc ttttactgat gagccagcaa aggcgaaatc
    19801 tttaccagca ggttgcttga ggaatccttc gctggcagca acttcatctt gtaacgcagc
    19861 atccagacgt cctgcagcca gatcggaata gaccaaatcc tggttggcat aggccaccac
    19921 atccacgcct ttactacgcc aggtctcgtt agcgtaagct tcctgggttg atccctgcag
    19981 cacaccaaca tgtttacctt tcagtgaatc cagcgttggc tgaatcggtg aacctttggc
    20041 cgcaatcaaa cgagaatctg cggcgtacag cttgtcggag aaggcaatct cctgctgacg
    20101 tttatcggta atggaaagcg acgaaataat agcgtcgatt tttttcgctt tcagtgaggg
    20161 gatcagcgcg tcaaagtcac tggcaaccca ggtacatttc acctgcatcc gtttgcacat
    20221 ctcgttaccg agatcgatat caaagccaac aaaatcacct ttagcatctt tcgatgagaa
    20281 cggtgcgtag gtggtatcgg ttccgatacg taccgtctcc ggtagcgccg catagctgga
    20341 agccgctgtg gagagaccga ctaacaaaga cagagcgaga atcgacttct tcatacataa
    20401 ccctcaagtg aaaatggctt ttttatgttg tgtattgtgt tgtgtgtttg caggctcttt
    20461 catgcaggtc ttatgccatc ttgacgacaa cagtaacatt caacgttaaa tatgttaata
    20521 agacgttgca ttattgtcct gaagttgaag atagcaggta tagcggttga atcgcagcgt
    20581 ttcgattgaa tggcagaaac aaaatgtcga ggatttgatc gcggttgcaa aattgccctg
    20641 aaacagggca acagcggagt tatgcgccct gccagcgggc aaagagatct tcaggaaggg
    20701 ttatcgcaaa ctggtcaaga acacgattaa ccgtctgatt tatcacatca tcaagggatt
    20761 gcgggcgatg ataaaacgcc ggaacgggag gcataatcac cgcaccgatt tctgccgcct
    20821 gagtcattaa acgcagatgg cctaagtgca atggtgtttc acgcacgcag agcaccaacg
    20881 ggcgacgctc tttcagcacc acatctgccg cacgggtcag taagccatca gtatagctat
    20941 ggacaatgcc ggaaagggtt ttgattgaac agggtaaaat caccatcccc agcgtctgga
    21001 aagaaccgga agagatgctg gcggcaatat cgcgcgcatc gtgcgtgaca tcggctaatg
    21061 cctgcacttc gcgcagagaa aaatccgttt cgagggataa ggtctggcgc gctgcctggc
    21121 tcatcaccag atgcgtttcg atatctgtga catcgcgcag aacctgtaat aagcgcacgc
    21181 cataaatcgc gccgctggca ccgctgatgc ctacaatgag tcgtttcata aatagttgcc
    21241 ctgtcagact tgcaggcaga ctttgcagga tttcggcggg agttgcaagt cagggtgcca
    21301 gaccggcacc ctcagcgaag gcatcatcct tcgttatgca tttcgagatt ttccacttcg
    21361 ttctgacgtt gcactgcttt ggcgtcatca ttacgtaacg tatcgaggaa atcgaggtag
    21421 ccctgatcaa catctttggt gacgtagacg ccgttgaaca ccgagcattc aaactgctgg
    21481 atatccggat tttcagcgcg aacggcgtcg atcagatcgt tcagatcctg gaaaatcaac
    21541 ccgtcagcac cgatgatctg gcgaatttca tcaacttcgc gaccgtgagc gatcagttcc
    21601 gtggcgctcg gcatatcaat accataaacg ttcgggaagc gaatttccgg tgccgcagaa
    21661 gcgaggtaca ctttcttcgc tccggcttcg cgtgccatct cgataatctg ctcagaagtg
    21721 gtgccacgga cgatggagtc gtcgaccagc aggacgtttt tatcgcggaa ctcggcgcgg
    21781 ttggcattca gtttacggcg cacggactta cgacgcagct gctggcccgg catgataaag
    21841 gtgcggccaa catagcggtt tttaacgaag ccctggcggt acggtttgcc cagaatacga
    21901 gcaatttcca gcgcgatatc acacgaggtt tctgggatcg ggatcaccac gtcgatatcc
    21961 agatcttccc attcgcgggc aattttctcg cccagtttcg tgcccatatt cacacgcgcg
    22021 ctgtaaacgg aaattttgtc gataaacgag tccgggcggg caaagtatac atactcaaac
    22081 aggcacggat tgctgaccgg attgtcagca cattgacggg taaacaactg cccttcttca
    22141 gtgatgtaaa tcgcttcgcc cggcgcgacg tcacgcagga aatcaaagcc cagcgtatcg
    22201 agcgctacgc tttcggaagc gaccatatat tctgtacggt tctcgtcaat atcacgtttt
    22261 cccagtacca gcggacgaat cccgtttgga tcgcggaaag caaccatacc gtggccgata
    22321 atcatcgcca cacaggcata cgcgccgcgg attaagcggt ttgtggcagc aatggcagcg
    22381 aaaatattgt cggcttccag cgggtagtgg cggaagttgt ccagctcgct ggcgaagata
    22441 ttaagcagaa tttccgagtc ggaagtggtg ttgatgtggc ggcgtttttc ttcaaacagt
    22501 tttttacgca actcgtgagc gttggtcaga ttgccgttgt gggcaagcgt aatgccatac
    22561 ggggagttaa cgtaaaacgg ctgcgcttca gaggcgctgg agctgccagc cgtggggtaa
    22621 cgcacatgac caatgcccat attgccctgc aaacgctgca tatggcgagc ttcaaataca
    22681 tcgctcacca gcccgttcgc tttacgcaaa cggaagcagt tattggcatc tatggtgatg
    22741 atgccggcgg catcctgacc gcgatgctga agcaccgtta aggcatcata aatcgactgg
    22801 ttaaccggca taacaccggc gataccgaca ataccgcaca tacgtctttt tcctcgttaa
    22861 gccacatctc agagcactta cgctctgggc aagaaacttg acgagctttg cagataatca
    22921 aaaaaccatc tgatgataaa actgaattgc gggatcagct gtgatttgct ccagtcttcg
    22981 cttttcgaca ccccggtaaa ggagtcgaga aagaagagaa tggcagcaac aatcaacaca
    23041 ccgcgcaacg caccgaaaca gacgcccagc acccgatcgg tgcctgacaa ccccgttttc
    23101 tccaccaact ggcctatcac gaagttcacg atagcaccaa cgatcagggt agcgataaac
    23161 agtaccgcga tggcaatccc atttcgaacc agttcgtctt caaagcccgt aaaccagact
    23221 gacaggtaag tgtagtaatg actggcaaca aagaaagcac aaccccatgt caccagcgat
    23281 aacgcttcac gaacaaagcc gcggatcagg ctaaccagag aggaaaaagc aatcaccgcg
    23341 attatggcgt aatcaatcca gaccatatgt gtcccacgat tttacgccct gtcatcctgt
    23401 tcggggcgca ttctaacaga aaaagaaaac gtttgcgtag ggatttcctt cccgcgcatc
    23461 aataaaaatg gcgctgaaaa aatattcaac gccatcgact ttttatgcct ttgcggcatc
    23521 gggcaatgcg tgtcggatgc ggcgtaaacg ccttatccga cctacggttc taacactgcc
    23581 caggcctgat aagacgcgcc agcgtcgcat caggcaagac tgtattaatt cggcgtatag
    23641 cccattacca cgccacttaa gccagaaagt tgcttcaact cacccagcga acctttcagc
    23701 ttatctttcg aggcatccgg cccaaccaga atacgggtaa ttttaccctg cactggcgtg
    23761 gatggcgacg tataaacccg ataaccggca ccgcgcagct taccgacaat ctcattcact
    23821 ttatcggcat ttttcagcgc acccagttgc acaacatagg ctttacccgt tggcgctgct
    23881 ttttcttcca cgaccggctt cggttctggc ttcggcgcag gtggtgcttc aaccttcggt
    23941 ttaggcggct ccaccggttt cggcttcggt ggggcgaccg gtgcaggttc cggttcaaac
    24001 tcggtgttat tggctgcaat agtggccgga tcgagcgacg gtgctgcggc atcacctgcc
    24061 cgcacctctt ccgctgcgcc ttccggcggc tgcgtcggta acgcctgggt ggcggctggc
    24121 atcatatcag gctcatcacg atcgcccgct ttcggcacca gcgggatagc cgcgaactca
    24181 tcctgataat gttttttctg cccgtccagc agccctggaa gtacaatcac ccccagcgcc
    24241 accagcacga tcgtgcccac taaccgattc tgaaacttac ttgccaccgc ttctcctcgc
    24301 gtcaatcact tccatgacat gtgcgaccgt gtggaaagaa ccacacacca gcacggtgtc
    24361 ttccgcttta gcgtccgcca ttgcggcatc ccatgcctgc gcaacgctat caaatgattt
    24421 gccgttaccc aaatgctcaa gcagttgttc tgccgtggca ccgcgcggcc cttccagtgg
    24481 cgcacaatac cagtcatcaa ccacgctttt caaccaggcc agagttccgg caatatcttt
    24541 atcatgtagc ataccgataa ccgccagcac gcgcccgttt ttcggtagcg ctttcatacg
    24601 cccggtgaga tattccgccg catgtggatt atgcgcgaca tcaaaaataa cgcgtggcga
    24661 ctcgctcaca atctggaaac gtcccggcaa aattgcgctg gcaatcccgt cgcgaatggc
    24721 attttcactg acttccagcc cgctggcacg cagtgccgcc agcgctgttg cggcattcgg
    24781 ttgcgggaca agcggcaacg gcagattttc cagcgtgccg tgagcatcgc taaacgccca
    24841 gtcatgatcg gtgacggaat agttccactc aacgccccga cgttgtaaca gtgcaccttt
    24901 ttcctgcgcc acatcagcaa tggtagaagg catttccggc tcaccgacaa ttgccggttt
    24961 ttcgctgcgg aagatgcctg ctttctcgcg accaatactt tcgcgatctg gacccagcca
    25021 gtcggtatga tccagcgcaa tactggttac taccgcgaca tcggcgtcga caatattggt
    25081 tgcgtccaga cgaccgccca gccctacttc cagaatcacc acgtcaagtt gtgcctgctt
    25141 gaacagccac aacgccgaca gcgtaccgta ctcgaaatag gtcagggaaa tatcaccgcg
    25201 tgccgattca atctccgcaa aagaggcggt gtgggccgat tccggcaatt cctggccctg
    25261 cacacgtacg cgctcggtat aacgcaccag atgaggcgaa ctgtagacgc ccactttgta
    25321 ccctgccgcc atcagaatcg actccagcgt acggcaggtg gtgcctttgc cattcgtacc
    25381 cgcaacggta aacacaaatg gcgctggttt caggacgcca agacgcgccg cgaccaggct
    25441 cacgcgctca aggccgagat cgatagtttt actgtgcagg ttttccagat aagaaagcca
    25501 cgaagccaga ggcgacgcgg cttgaggagt gcgtttgata atcatggtat ccgctgattc
    25561 gttacggtga gaatagcaaa agggcagagc cagtggccct gcccttatca gttatcaggc
    25621 ctcaggttcc tgatccggta ccgggggtac cactacgcct tcacgcggcg cttcaggatt
    25681 cggcgctggc agattcatca acttcgccag aatgctcgcc agtttcaggc gcatttccgg
    25741 acgacggacg atcatgtcga tcgcgccttt ctcgatcagg aattcactgc gctggaatcc
    25801 aggcggcagt ttttcgcgaa cggtctgttc gataacacgc ggaccggcaa agccgattaa
    25861 cgctttcggt tcagcgatgt tgagatcgcc cagcatggcg aaacttgcag aaacaccgcc
    25921 catcgtcggg tcggtcagca cggagatgta cggcaagccg cgctcctgca tttttgccag
    25981 tgccgcagag gttttcgcca tctgcatcag cgacatcagt gcttcctgca tacgtgcgcc
    26041 accagaggcg gagaagcaga tcagcgggca gttatcttcc agcgcctgct caacggcacg
    26101 cacgaaacgt gcacccacaa cagaccccat tgaaccgccc ataaaggcga actcgaatgc
    26161 cgcagcgaca accggcattc catacagagt gcctttcatc accaccagcg catctttttc
    26221 gccggtttct ttctgcgcag atgccagacg gtctttatac ttcttggagt cacgaaactt
    26281 cagcacatct ttcggctcaa gctcgctacc cagctccaca aggcttcctt catctaacag
    26341 gctatgcagg cgattacgcg ctgtcatacg catgtgatgg tcacacttcg gacagacctc
    26401 aagattacgt tccagctcag cgcggtataa aacctgaccg cagctatcac acttagtcca
    26461 caccccttca ggaatgctcg ccttgcgggt gggagtaatg ttgcttttaa ttcgttcaat
    26521 ccagctcatt agggaccttt ctgtctgaac ctggttcgat gccagtttta tctttgggga
    26581 cgcataatgc catttttgcc cccaacagac catgaatgtt gcacattaaa acataacagc
    26641 ccgaaacttt ggataaaaaa gtggtcgaac cgcggagtta ctttttattt tgcggcgcgt
    26701 gccgcagcgc gtttgtgacg gattatttcg atgacgcccg gcaaaataga aaccacaata
    26761 atcccgacga tcagcagctt aaggttatcc tgaaccatcg ggattgtacc gaagaaatag
    26821 cctgcgtagg taaaaagcag tacccacaac agtgcgccga tcacgttata agcggcgaaa
    26881 tgacggtacg acatgtggcc cattcccgca acaaacgggg cgaacgttct gacgatcggc
    26941 acaaaacggg cgagaataat cgttttgccg ccatgtttct cataaaactg atgggttttg
    27001 tcgagataac tgcgacggaa aattttcgaa ttgggattac tgaacaactt ttcaccgaac
    27061 aaccgcccga tagtgtaatt gaccgcgtca cccacaatcg cggcgatcag catcagcacc
    27121 accatcatat ggacgttgag atcgttggtt tccagtgatg ccaacgctcc ggcaacaaac
    27181 agcaatgaat cacccggtaa aaacggcgtt accaccagac cggtttcaca gaacaaaatt
    27241 aagaacaaaa tggcataaac ccagacgccg tactccgcga ccagctctgc cagatgcacg
    27301 tcaatgtgca ggataaaatc gatgaggaaa taaatcaggt ccatatttgc ctatgccttg
    27361 tactcgtcat ctttcaggct gtaactacgt tggctacagc ctgaaatgtt ccgagcatta
    27421 ttctcgttag tccgccagaa atagcgggcc cattggcggt tttggaagat cataccggtc
    27481 agggtaatcc accgcgacca gatacagccc ttccgctttt gccgttgctg ccgccagcgt
    27541 tctgtccttt gccgccagca actctgctat ccagctctcc ggctggttgt gggcaccgac
    27601 ttccatcagg ctgccgacaa tattcctgac catatgatgt acaaaagcat tcgctttaat
    27661 atctaccacc acataaggac cgtgacgcgt gacattaata tgcataacgt tgcgccacgg
    27721 ggttcgggac tggcactgca ccgcacggaa cgaggtgaaa tcattctcgc ccagcaagca
    27781 ttgcgcagcc cgatgcatcc gttcagcgtc cagcggttcg taaaaatggg ttaccccttt
    27841 actcagtacc gccgggcgca gccgatgatt gtagatgatg tagcgataac ggcgagccgt
    27901 ggcgctaaat cgggcatgaa aatcatcagg tacagcttta acccaacgca cagcgatgtc
    27961 accaggtaaa ttcgcattta cgcccaaggt ccacgccgcg tctttgcgca gcgcggttgt
    28021 ttcgaaatgc acaacctgcc cggtaccgtg tacccctgcg tcagtacgcc cggcgcagaa
    28081 gacggtgatg ggttcgttcg ccacctggga gagcgccttt tccagcttct cctgcacact
    28141 gcggacttcg ttctgccgtt gccagccgta atacttactg ccgtcgtact caatgcccag
    28201 cgcaatttta taaactggcg gttgttgctg gtcggacatt agtacagata ctcctgcacc
    28261 agtttctcgg cgatttttac tgccatcagc gcgccgccaa agcgaacgtt atcggccacc
    28321 gaccagaact ggacttgctc cggcataccg tagtcattac gaacgcagcc aacagaaaga
    28381 tgcggcgtac ccgaagcatc acctacctga gttgggaatt cgttctcttc agagagcaca
    28441 atatcttcgc cttgaacaaa cgcatcacgc gcttcttctg ctgccagtgg acgcagagct
    28501 tcaaagttga ccatctgggc atgaccgtag aataccggtg cctggacgac gctagccgaa
    28561 atcatcagcc cttcgtcctg caggattttg cgtacttcgt caacgatacg acgttcttca
    28621 cgcacgctac cttcgctatc cggcagtaac ggcagcatgt tgaacgccag ctgacgcccg
    28681 aagaaatctt cttcgtcaat cggaatgccg ttgagcaatt tcgcactctg ccccgctaac
    28741 gcatcgaccg cttttttgcc ctgggcggag gctgaaatca ggctggtaac gctgatacgt
    28801 gataaaccgc cctgatcgat taacggtttc agtgccgcca gcaactggct ggtcagactg
    28861 tctggtacgg cgatgacatt ccggttccgg taatctgtca gtacaaacgg gtttacttcc
    28921 ggcaccacca gcggtacgtc gggttcgaga gcaaacaatc cactgctgtc gatcaccagg
    28981 caacctgagt tggtcgcttc ttcaacccag gcagcggtag cttctttgcc tgcgacaaaa
    29041 aatgccaact gcgcctgcgt ccagtcgaat tcagcggcat cctgcacggt gattgtctta
    29101 ccaccaaagc gcagttgttc gcctgcgctt tcgttacgtg ccagtgcata aatttcccca
    29161 accgggaact gacgttcagc cagcgtttca agcagggctt cgcccacagc gccagttgcg
    29221 cccaggacgg caatgttcca gccttcagac atggtggttt actccagaaa tagcaaagct
    29281 ccctgccaat gttacagcag agagcatgaa gaagagatta acgtgccgga tgatgaacgg
    29341 cgttaaaacc cagtttacac agcaatgatg ccgcactggc gtcatcacaa attacataca
    29401 gagatgacca ttcacggcgc tcaagatagt ttttgcgcag tttatcgaac tcacccggta
    29461 tcccggcgac tttacgcagc ggtgcgtcat cgcggcgcac atcatacacc aaatgcacca
    29521 gccttttcag cgtcggttga tcgagcgggc catgcagcgt aatgcgacca aactctggcg
    29581 caggcagtaa tgtatccagc gcaacgtgct gttcatgccc aataaacttg ctataagctt
    29641 caaacacttg cgtagtaccg cgtgctttac cttccagggt atagcctgcg atatgcggcg
    29701 tgccgatatc cacttttttc agcagctcga cgttgagttc cggttcgcct tcccagacat
    29761 ccagcactac gcttaacttc tggccttcat tcaggcaggt cagcaacgca gtattatcga
    29821 cgactgcgcc acggcaggcg ttaatcagaa tcgctccggg cttcaggcta cggatcagtt
    29881 tttcatccgc cagatgtagc gttttgtacg gaccatcttt aaagagtggc gtatggaaag
    29941 tcagaatatc cgcgcgctgg actaactcat ccagcgagcg gaaatcaccc tcatccccac
    30001 ggtcggcgcg aggcggatcg caaagtaagg ttttaatccc taacgcttcc agtcgcgcct
    30061 gcaatcgacg tccaacgtta ccaacgccca cgatccccac cgtacggtcg tacagtgaaa
    30121 atccatcgcg ttcggcaagc atcagcaggg aggagaaaac atattccacc accgcaatcg
    30181 cattacagcc aggtgcagcg gaaaaaccaa ttcccgcctg cttcaaccat gcttcatcga
    30241 catggtcggt ccccgcagtg gcagtgccaa caaatttaat gggttttcct gccagcaaag
    30301 attcattcac tttcgtgacc gaacgcacca tcagcgcatc cgcgtctgcc agttgagcga
    30361 cggggattgg acgcccggga accgcggtca cctcacccaa acggctaaat aagtcgcggg
    30421 cataaggcat attttcatca acaaggattt tcacgtttgt gttacctgta tgagacgaga
    30481 gttaaccgga caagtgtgcc ataatctcgc ggccaggcat acttgcgaag atttcaggta
    30541 taaggatacg taatgataca acctatttcc ggccctcctc ctgggcaacc accaggtcag
    30601 ggagataatc tgccgtctgg cacgggcaat cagcctttat ccagtcagca acgtacttcg
    30661 ctggaaagct taatgacgaa agtgacctca ctgacgcaac agcaaagagc agaactgtgg
    30721 gcgggtatca ggcacgatat tggtctgtcg ggagattcac cgctgctttc gcgtcacttc
    30781 cctgccgctg agcataatct ggcgcaacgt ctgctggccg cgcaaaaaag ccattctgcc
    30841 cgccagcttt tagcgcaatt aggggagtat ttacgtctgg ggaataatcg tcaggcggtc
    30901 acggattata tccgtcataa ctttggtcag acgccgctga atcagctctc accggagcaa
    30961 ttaaaaacca ttctcaccct gttgcaggaa gggaagatgg ttattccgca accacagcag
    31021 cgcgaggcga ccgaccgtcc tttattaccg gcggagcaca atgcgctaaa acagctggtg
    31081 accaaacttg cggcggcaac gggggaaccc agcaaacaga tctggcaatc gatgctggaa
    31141 ctttccgggg tgaaagatgg cgagttaatt ccagcgaaac tgtttaacca tctggtgacc
    31201 tggctacagg cgcgtcagac gctaagccag caaaatacgc cgacgctgga atcactacag
    31261 atgacgctaa aacaaccttt agatgccagt gaactggcgg cgttatcggc atatatccag
    31321 caaaaatatg gtctttctgc gcaatcatcg ctttcttctg cccaggccga ggatattctt
    31381 aatcagcttt atcaacggcg ggttaaaggg attgatccgc gtgttatgca accgctgctt
    31441 aatccttttc caccgatgat ggacacgttg caaaatatgg caacgcgtcc cgcgctgtgg
    31501 atactgttag tcgcgattat cctgatgctg gtctggctgg ttcgttaacc ccgacgaaat
    31561 gacagtatcg tgacaataat acccagcacc gcagagatcg ccccggcaag aaataccgaa
    31621 gagtaaccaa acgtggtcgc cagcattccc gccagcggcc cggagacgcc gagggcgata
    31681 tcctgaaacg cggcgtaacc gcccagtgcg gtgccgcgaa cttgtgaggg gacgcgttta
    31741 accacctcca cgcccagcgc aggaaagata agcgaacatc cggctccggt taacgccgcg
    31801 cccgctaatg cgacccatgc acctggggct tgccagagca gcaacaagcc caccgtttct
    31861 acaagcagag agacaatcgc cactttcacg ccgccaaaac ggtccggcat ccagccaaac
    31921 atgacgcgca tcacgacaaa tgcgccgcca aacgcggtaa gagtaaagcc cgacaacgct
    31981 ccacagcgat ggacgttctc ccgccagggc cggtactttg cgcactgtgc cgttacaggc
    32041 ccacgccagt acgggtaata ccattgtggt gatcgccagt gcggcaaaac cgtaatggct
    32101 atgaatcaac aggccaagcg gagcaccaac agcgagggca ccgtaaatcg ccattccgtt
    32161 ccatgacatc actttgccag agtgttttgg ccctacgatg cctagtcccc aggtcagagc
    32221 gcctgtcagt aactggcttt caccaaagcc aagaatcaaa cgcccgacga ccaacagagc
    32281 aaatttgaac ggtgcggaga caggcaaaat cgccgccagc agcaacgcgc cgccagccag
    32341 accacaagct aacattccct gaagcgccga acgttttgca ccatattgat cggccagtcg
    32401 cccggcgtaa ccacgcgtca gcaccgtagc cagaaactga atcccgacgg caatgccgac
    32461 catggtattg ccatagccca gttcatgatg aacaaacagc gggataaccg gcaacggcaa
    32521 ccctacggtc atgtaggtga gaaaaaccgc aaaagcgatg cggaagagcg aaaaattggc
    32581 agaagatcgt gtttcggttt ggcttacagc agtcatgcat tactccagaa tgcagcgcaa
    32641 ggcgaggagt atccccgtct catctctctg gtttcagggt tacggtgcgt tggcaggatt
    32701 taacgcgtac gtcttttcag aaggaaatcg acaaagcggg aagtttgcct ggaactggcg
    32761 gcgattgtca atgatgtgaa aaagggaacc atcaggttcc cttttgcgtt agtgccggag
    32821 gccgcattgg cgcgtaacgt cggatgcgac gctggcgcgt ctactccgac ctactgcgaa
    32881 ttaatctttc agcttgcgca ttaccagcgt ggcgttggtg ccgccgaagc cgaagctgtt
    32941 agacataacg gtggtcagtt cgcgatcggt cgtttcggtc acgatgttca gacccgcagc
    33001 ctgctcgtcc agctcttcaa tgttgatgct cggggcgata aagccgtgtt ccagcatcag
    33061 cagagagtag atagcttcct gtacgccagc agcgcccaga gagtgaccgg tcatggcttt
    33121 ggttgcagaa atcgccgggc tcttatcgcc gaacacttca cggatagctg ccagctcttt
    33181 cacgtcgcca accggagtcg aagtaccgtg ggagttcagg taatcgattg gggtatcaac
    33241 gccatgcatc gccatcttca tgcagcgtac tgcgccttcg ccagacggag caaccatgtc
    33301 tgcaccatca gaggttgcgc cgtagccaac gatttcagca tagatgtgag caccacgcgc
    33361 cagcgcgtgt tccagctctt caaccactac cataccgccg ccgccagcga taacgaaacc
    33421 gtcacggtga gcgtcgtaag tacgggaggc tttttccggg gtgtcgttgt atttagtaga
    33481 cagcgcaccc attgcgtcga attcgcaagc catttcccag cacagctctt cgccgccgcc
    33541 agcaaacacg atgtcctgtt tgcccagttg gatctgctct actgcgttac cgatacagtg
    33601 tgcggaagtc gcacacgcgg agctgatgga gtagttaacg ccatgaattt taaacggggt
    33661 ggcgaggcag gcagaaacgc cggatgccat cgctttggtg accacatacg ggccaaccgc
    33721 tttcaggccg cgcgggccgc gcattgcgtc agcgccgaac acctggaaac gcggggagcc
    33781 gccgccggaa cctgcaatca ggccaacgcg cgggttattc tggtaagctt ccggagagag
    33841 gcccgcatca gcgattgcct gctccataga aaggaatgca taaatggatg cgtcgctcat
    33901 aaagcgcaca actttgcggt caatgaggcc agtggtatcc agttttacgt tgccccagac
    33961 gtggctacgc atgccggaat ccttcagctc ctgagagaaa gtgatccctg aacgtccttc
    34021 acgcagagat gccaggactt cctgctggtt attaccgatg ctggaaacaa tgcccaggcc
    34081 agtaatcact gcacgtttca ttcaatacct ctgtaagtcg cacatagagt aagtttcgaa
    34141 tgcacaatag cgtacacttg tacgccgaac aagtccgatc agccatttaa tagagaaatt
    34201 tgcgcagcct tacacacatc gctaagatcg agccaccgcc tgtaagacga gtaacttacg
    34261 tgaaacacta ctccatacaa cctgccaacc tcgaatttaa tgctgagggt acacctgttt
    34321 cccgagattt tgacgatgtc tatttttcca acgataacgg gctggaagag acgcgttacg
    34381 tttttctggg aggtaaccaa ttagaggtac gctttcctga gcatccacat cctctgtttg
    34441 tggtagcaga gagcggcttc ggcaccggat taaacttcct gacgctttgg caggcatttg
    34501 atcagtttcg cgaagcacat ccgcaagcgc gattacaacg cttacatttc attagttttg
    34561 agaaatttcc cctcacccgt gcggatttag ccttagcgca tcaacactgg ccggaactgg
    34621 ctccgtgggc agaacaactt caggcgcagt ggccaatgcc cttgcccggt tgccatcgtt
    34681 tattgctcga tgaaggccgc gtgacgctgg atttatggtt tggcgatatt aacgaactga
    34741 ccagccaact ggacgattcg ctaaatcaaa aagtagatgc ctggtttctg gacggctttg
    34801 cgccagctaa aaacccggat atgtggacgc aaaatctgtt taacgccatg gcaaggttgg
    34861 cgcgtccagg cggcacgctg gcgacattta cgtctgccgg ttttgtccgc cgcggtttgc
    34921 aggaagccgg attcacgatg caaaaacgta agggctttgg gcgcaaacgg gaaatgcttt
    34981 gcggggtgat ggaacagaca ttaccgctcc cctgctccgc gccgtggttt aaccgcacgg
    35041 gcagcagcaa acgggaagcg gcgattatcg gcggtggtat tgccagcgcg ttgttgtcgc
    35101 tggcgctatt acggcgcggc tggcaggtaa cgctttattg cgcggatgag gcccccgcac
    35161 tgggtgcttc cggcaatcgc cagggggcgc tgtatccgtt attaagcaaa cacgatgagg
    35221 cgctaaaccg ctttttctct aatgcgttta cttttgctcg tcggttttac gaccaattac
    35281 ccgttaaatt tgatcatgac tggtgcggcg tcacgcagtt aggctgggat gagaaaagcc
    35341 agcataaaat cgcacagatg ttgtcaatgg atttacccgc agaactggct gtagccgttg
    35401 aggcaaatgc ggttgaacaa attacgggcg ttgcgacaaa ttgcagcggc attacttatc
    35461 cgcaaggtgg ttggctgtgc ccagcagaac tgacccgtaa tgtgctggaa ctggcgcaac
    35521 agcagggttt gcagatttat tatcaatatc agttacagaa tttatcccgt aaggatgact
    35581 gttggttgtt gaattttgca ggagatcagc aagcaacaca cagcgtagtg gtactggcga
    35641 acgggcatca aatcagccga ttcagccaaa cgtcgactct cccggtgtat tcggttgccg
    35701 ggcaggtcag ccatattccg acaacgccgg aattggcaga gctgaagcag gtgctgtgct
    35761 atgacggtta tctcacgcca caaaatccgg cgaatcaaca tcattgtatt ggtgccagtt
    35821 atcatcgcgg cagcgaagat acggcgtaca gtgaggacga tcagcagcag aatcgccagc
    35881 ggttgattga ttgtttcccg caggcacagt gggcaaaaga ggttgatgtc agtgataaag
    35941 aggcgcgctg cggtgtgcgt tgtgccaccc gcgatcatct gccaatggta ggcaatgttc
    36001 ccgattatga ggcaacactc gtggaatatg cgtcgttggc ggagcagaaa gatgaggcga
    36061 taagcgcgcc ggtttttgac gatctcttta tgtttgcggc tttaggttct cgcggtttgt
    36121 gttctgcccc gctgtgtgcc gagattctgg cggcgcagat gagcgacgaa ccgattccga
    36181 tggatgccag tacgctggcg gcgttaaacc cgaatcggtt atgggtgcgg aaattgttga
    36241 agggtaaagc ggttaaggcg gggtaatctg ctctcgcagt gtttgccgga tgcggcgtga
    36301 acgccttatc cgacctacgt gtgacgtgta ggcctgataa gacgcgataa gcgtcgcatc
    36361 aggcatggtg ctccagacgc cgcaacttac tgctgtgacg cctgctgaaa caggttttcc
    36421 cacatatcgg tcaccagcgc ctggtcacgc ggcgacaatt caccggcacc aatggctttt
    36481 tccagactct ggctaacggt cgtatgcacc gcctgagcgg agtggtcatc accactttcc
    36541 agttctgcga tggctaatgt caggtggcca cgcaaatacc cactggcaaa caactcatca
    36601 tcactggcat ggtcaaccat accgtcgatt aatgccagaa tgcgtgattc aaactccgcg
    36661 atcatcttct ttcctcattg taaaacgtgg cgctggcttc agttgagcgc ttccggccac
    36721 gggaactgtt ccgccgtaag ttccggcgtg tgataataat tttgtagtgc tttaatgaag
    36781 cgtgccggac gttcgggaat gccgtttgcc agataatcca ttacctgcgc atgtacccgc
    36841 cgttgaaaca ccacgcggtc cggttcgaaa tccccttcca gattgtcgca gctaacatta
    36901 aacggatatc ccgccgccac gcagaacaac cagtccagcg cctgcggctt cacttcaaca
    36961 tcttcaaact gactttgcgt ctgggcatcg cgtccgtccg ggcaatacca gtagccgaaa
    37021 tcaaccagct cacggcgcgc tttcccggca atacaccagt gcgaaatctc atgaattgcg
    37081 ctggcataaa agccatgagc aaagacgatt cggttatacg gtacttccgc atcagcagga
    37141 agatagatcg gttcgtcgtc gcctttaatc agacgggtat taaaatcatc ggcaaagcag
    37201 ctattaaaaa tttcaatcaa ctgctcatag tggtgtgtac tgttcattag ttcatcccca
    37261 accagtggag gatctcctgt ccgtggctat cataaagtaa tttggcactc atcaccgccg
    37321 agacgataac gatcatcggg cggatcagtt tttgtccttt gctcaacacc agtcttgaac
    37381 ccatgcgcgc gccgaggaac tgccccacca gcatgacaaa gcccgtcgcc caaatcactt
    37441 tgccaccgag aataaacagc agtaaaccgc cgatatttga cgttgcgttg agtaatttgg
    37501 cgtgagccgt ggctttggcg aggttgaatc cgcacagcgt aacgaaggcc agcgcgtaaa
    37561 acgatccggc agctggccca aagaatccgt cgtaaaaacc gacgcagcca cctgcaatta
    37621 acgcgaaggg taaaccgtac atccggcgct ggcgatcttc ttcacccaat tttggcatca
    37681 gcaagaaata gaggccgata caaatcacca gaatgggcaa aatctgtcgt aagacatcag
    37741 cctgaacgta ttgcaccagc agtgcgccgc tcattgagcc gacaaaggtc atggcgatat
    37801 tgagtttctg atcgcttaaa ctaaccactt tgcggcgaat aaagtagata gtggcggaaa
    37861 tagagccgcc gcaggcttgc agtttattgg ttgccagcgc attagcggga gacatccccg
    37921 ccgccatcaa tgccggaatg gtgagtaacc caccgccacc ggcaatcgag tcgataaatc
    37981 ccgccagcat ggcgacaaaa aagagaactc ccagcaacag cggggaaacc ataaacaggc
    38041 tattaaacgt ttccattaga tcacgtgctc atccagtagc gcctggcagg aaggcggcaa
    38101 cggaggcggt gtcttcttct caggctttgt tgttcccggt tttggaggtt caaaccagct
    38161 ttgcagttct gccccgcaac catcgcctgg tggcggtaaa ggttgatctt cacactccag
    38221 actatcggca ggacaacgta atcgtacatg catatgcgcg cgatgctgga accagggccg
    38281 cactttgcgc aaccagtcgc gatcggtgcc cgcatcaaag caaagttgtt gtttaatcgc
    38341 cggattaaca aaaatgcgtg tgacgtcttt gtcctgggcg gcgagtttga tcaagctgaa
    38401 aatttctggc ttccacagcg tggagacaac gtgtttaccg tcgcgggaaa ccaaatctaa
    38461 cgcttgcggg cgcaaaagtt gcgcggaggt ccagcgtgtt ttcggcagtt gtaagaagat
    38521 atcaacatcc agtccggtct ggtggctggc atgaccgccg ttgaaacgcc caccagcggg
    38581 catccccata tcgccaatca gcaccgtacc catgcccaga ttgctcacct ggctactcag
    38641 acgctgaata aacatcacca gatccgggtg accgaaatag cgacgctgat cggtacgcat
    38701 gacctgataa tgttcggact gtatcggcaa cgtgtcagcg ccgacaatac agccattaga
    38761 aaaactgcct atcgattgtg cgctacccgg cacaggttgg gttatttttt gccacggcgt
    38821 cgctgccagg ctggcgctac tggcaagcag ggccagcagc gcaatcgcgg ttttgttcat
    38881 tttttaccag cgtggaatat cagtcttcac atcggcattt tgcgcccgct gccgtaacag
    38941 gtgatccatt aatacgatcg ccagcatcgc ttctgcgatc ggcacggcgc ggatcccgac
    39001 acagggatcg tgacggcctt tggtgatcat ctcaacttct tcgccaaagc ggttaatggt
    39061 acgccccggc acggtgatac tggaggtcgg tttcagcgcc atatgagcaa tgatttgctg
    39121 cccgctgctg ataccgccga gaatgccgcc cgcatggttg ctctggaaac cgtctttggt
    39181 gatttcgtcg cggttctggc tgccgcgcag cgccactacg tcaaaaccgt cgccaatttc
    39241 cacgcctttc accgcgttga tgctcatcag cgcatgggcg atgtcagcat ccaggcggtc
    39301 aaagaccggc tcgccaagtc cggcggggac gccactggca acaacggtga ctttcgcgcc
    39361 gatggagtcg ccctcttttt tcagcgcgcg catcagttca tctaacgcgt cgattttgtc
    39421 cgggtccggg cagaagaacg gattttgttc aaccagcgac cagtctttga tttccagcgg
    39481 aatgtcgccc atctgggtca ggcagccgcg gatttcaata ccaaatttct cggcgagata
    39541 ttttttggca atcgcccctg ccgccacgcg catggcggtt tcgcgggcgg aagaacgtcc
    39601 accgccgcga taatcgcgca gaccgtattt ttgttcgtag gtgtaatcag catggcctgg
    39661 acggaagacg tctttaattg cgctgtaatc ctgagaacgc tggtcggtat tttcgatcaa
    39721 caagccaatg ctggtgccag tagtaacgcc ttcaaaaaca ccggagagta ttttgacctg
    39781 atccggctcg cggcgctggg tggtatagcg cgatgtccca gggcgacgac gatcaaggtc
    39841 atgttgcagg tccgcttccg tcagcggaat gcctggcgga acaccatcga cgatgcagcc
    39901 gagcgccagc ccatgcgatt cgccgaaggt ggttacgcga aagagttgtc caattgtgtt
    39961 tccagccatc acggctccgt tattgttgtg tttgcgtgtt tacttaatct ttataaatcg
    40021 cgaaatattc acgggccgcc agcagctgct ctttggtgag cataaacaca ccatcgccgc
    40081 cgttatcaaa ctccagccag gtgaacggaa catccggata ttgttccata agatgtacca
    40141 tgctgttgcc gacttcacaa atcaacacgc catcatcagc aaggtaatct gccgcgtttc
    40201 caagaatgcg acgcgtcagt ttcaggccgt cggtgccaga tgccaggccc agttccggct
    40261 cgtggcggta ttcatttggc aggtcggaca tatcttccgc atcgacatac ggcgggttag
    40321 tgacaatcag atcgtactgc actttcggca agtcgcggaa cagatcggaa cgaatcggaa
    40381 tgacgttgtg gatcagaccg tgttcttcga tgttctgttc ggcaaccgcc agcgcgtcgg
    40441 gagagatatc taccgcgtcg acttccgctt ccgggaaggc ataagcacag gcaatggcga
    40501 tgcagccact accggtacac atatctaaaa tatgctgcgg ttgcttgctg ataagtccgg
    40561 caaatttatt gttgatcagt tcaccaatcg gcgagcgcgg caccagcacg cgttcatcga
    40621 cgtaaaattc atggccgcag aaccacgctt tgttggtcag gtaagccacc ggaatgcgtt
    40681 cgttgacgcg gcggatcacg cgctcaacaa tgcggtgttt ttcgctggag gtcagacgcg
    40741 cggtgcgcat atcttccgga atatccagcg gcaggtagag cgaaggcaac accagttgca
    40801 cggcttcatc ccacggatta tcggtgccgt gaccgtacca gatatttgcc gcgctgaagc
    40861 ggctaaccga ccagcgcaac atgtcctgaa tggtttgcag ctcatttact gcttcatcaa
    40921 cgaaaatttt atccacgtat tcctccaggg catgatcgca ataatttcgg cggctagttt
    40981 gccatgaaga tgacgataaa tcagcattca cgcgcggtga gtgaggaaaa atacgtttta
    41041 aacgatcgat tgtgctacga gtcgggtaaa ctgtaggaaa attagaaata gagacagata
    41101 aatgaaaaag aaaacaacac ttagcgagga ggaccaggct ctgtttcgcc agttgatggc
    41161 ggggactcgc aagattaagc aggacacgat tgtccaccga ccgcagcgta aaaaaatcag
    41221 cgaagtgccg gtgaaacgct tgatccagga gcaggctgat gccagccatt atttttccga
    41281 tgagtttcag ccgttattaa ataccgaagg tccggtgaaa tatgttcgcc cggatgtcag
    41341 ccattttgag gcgaagaaac tgcgccgcgg cgattattca ccggaattgt ttttggattt
    41401 acacggtctg acgcagctgc aagctaagca ggaactgggc gcgttgattg ccgcctgccg
    41461 ccgtgaacat gtgttttgcg cctgcgtgat gcatgggcac gggaagcata ttttgaagca
    41521 acaaacaccg ctgtggctgg cacaacatcc gcatgtgatg gcctttcatc aggcaccaaa
    41581 agagtatggt ggtgatgcgg cgttgctggt attgattgaa gtggaagagt ggctgccgcc
    41641 ggagttaccc tgagaagttt agcgcgttgc gccatgtacg gggaatggca gggtcaaatg
    41701 accctgcctt gtgaaaaaga cgcactagta catattcagt atgtaagtga accggcaggt
    41761 ggcatctatt ggctgttgcg ccgttgtgtc cacgcgtagc ttcatatcat gcagttgcct
    41821 ggacgtgatc ggttgcggct ctggcgtcac tgtcactttg gcatcgtcac agcccgaagt
    41881 ggagaaggtc atctgctgtg cgacaaaacg gcagtaagca ccacgctcgt catatttact
    41941 accatcacac agttttccac cttccatcag ataagtgggc agcgtttgtt caatcacagc
    42001 acctgttgat tgtatcctga ctattttgtt atgcacgacc gtattttcat tccaggagac
    42061 atcataagga tctaaaggta ttgcgctaca gtcagaactg acttttgtcg ggccaatata
    42121 gggaacatta ttacctgtat atattgcctc cgttgcgtcc tcactcttta catacgagga
    42181 aactatccac ggacaaccat gcatatcacc gtcatagaaa actgtgactt tcatttccac
    42241 gcaggcccag gtcgtttgtg gggtgcgagc atcatattct tcaaggcccg ggcaacgtga
    42301 agttggtaat atggttcttg cgtcttctaa atgcagttcc ggagcagtac cttgaccagt
    42361 ccatgatact agtggcacat tcaaaatcgc aactgacctg gattgatata ccccatattg
    42421 cgcgccttct ggtgtgagga tagaaagatt aaactgaaaa cctgtgagca ctcccattga
    42481 tcccgcagga atgtataacg tcgcgatctt tttcgcggcc tgcactcctg tgctgaggca
    42541 aatgagcagc aaaaagtaaa agacataacg tagaattttc attttaaata tactcgaagg
    42601 tagcggtcag gctggcagtg aattcaccga tcgtcacatc gcgaccgctt ttcgcctgga
    42661 tcctggcccg gaaattaata tcattgttgc cgttggtaag cgcaaaagtc gccccggtag
    42721 tgttgttaat atccacccgc ttgccgtcga gcgtttccat ccctatcccc acgccctgcg
    42781 cggtggaggt gtcatcgact gccagaaaac ccggctgacc gctgtcttcg gtgccgctca
    42841 gcgtgacttt gacgttgtag cccgctggcc ctttgcagtc tttcagttta aacacaaccg
    42901 gcagatgcac agactgtccg gcgactatca aatcccggtt gctgacggtg ggaaaatgga
    42961 tttctgccag attggcacca tccaccacca gcgtgcagga tttactcagc aggttgccgt
    43021 aaaaatgcag gttgttatcg actgcccgtg cccccgtgct ggcggtcagc agcaggcctg
    43081 ctaacagcgc ataaccgggt gaataaatgt gtgctgtttt cattggtaat ccacccgtaa
    43141 ggttgcgtaa gcagaaaagg tcgtgtccgt cagcaccgca tcagccgctt tgaccggtac
    43201 cgcctccagc tttggcttct ggctaaaatc ggtggcgtta atggaaagcg gctcgttgag
    43261 cttaaaacgc tgaccgtcat gctgaagctc aattccgaag cccggtacat ccgtctcaat
    43321 agcagagtcg ttgtaggagg tggcagtccc cgtccatgag agtgtcatat tccactcggg
    43381 atcgcggaca tcagactcac agctcagttc gtagtccact tcctgacgac cataattgcc
    43441 gtcaatgctg tcgataatga gatcgcgaaa ttgcacttcg atggtttgcc cgccgctgat
    43501 gctacaggcg ggcggcgaca tcagcgtacc gtggaatgtg atgtcatctt ccgccgccag
    43561 cacagccccg caaccagcgc acagtaaaag gagcaggaat tttttcattg gtaatccacc
    43621 accatggtca tcgtggcgtt aaactccgac ggcgtcagtt gcaccccgct ctgttttacc
    43681 ggcaccgctt tcagctgagg ctgtgtgttg acattaaaag gcgtccagtt attttcacca
    43741 actgcaaaca gggagttgtc ggcggcgttt tctatccgca cccccaggcc ggcgatatcc
    43801 gtgctgagaa ccgtttctcc gttgatcacg gcggtattgc ctttgagctg catccgtaag
    43861 tcatcgctga ctttattacc gcagttgagg gtgtatttcg catcctgttg gtaattggta
    43921 ccatcgatcc tgttgatcac cacattgcca aattccaccg cgcttccctt cacggagcac
    43981 ggcggcgggc catcgaccag gattcgcagc gtcagatcca cctgtttgat atcgctgcca
    44041 gcaacggcta aaccgctgac accccatccg gccagtacca gtagccccgc gcccgctaaa
    44101 aacttgtgac tcataagtcc ctcccggcat cagcctttgc ggctctgctc ttcatccacc
    44161 tggcagttgt tgccggtgca tttgaagtac agcggcatcc gcgcgccgta gtcgttgatg
    44221 taggtcagca ccggcacgct gtccatcttc gcattcagcg gtgcggaggt tttcggctcc
    44281 atcatcagcg gtgtgaatcc cgccggcggg ttgccgcctt ttttagcgct ggcgttgctg
    44341 atgatcacgt agtacggcgt cgggttattg acggtgaagt cctggccgtt acggatgagc
    44401 gtcaccttat gctgccacgg acttttcatg ctggtgttct ccaacgcctt cggtcgccag
    44461 aacagtttga tacgcgtctg cagcgcaatc tgcagtgtgt tcggtttatt tgatttcggc
    44521 ggaatttcgc gcacgttgaa atagaacacg ctctcgcggt cggccggcag tttgttgata
    44581 tccggcagcc cctgcacctt cacctggccg ttcatcatcg catcgatgcg ctgcactggc
    44641 ggaagcaccg caagcggcga gctgattttg ttgccctttg catcctctat ccagctctgc
    44701 gccagataag gcagtttggg atcgttgttg cgcagcgtga cgctgatgga cttgtcgctt
    44761 tcgttaaaca ccagccgtgt cctgtcgggc gtcaccgaag cgtggctgga gaaactgacc
    44821 gctgtcgcca gcagcgccag tgccgggaca acgatttttg cagtgttagt cagattaaac
    44881 atggatcatt ccttaatcac gttactggtt tactgaaatt gaagtcgggg gttccgttgg
    44941 cgtcacccgc tgtgtctgtt cctgaatcgc cggtttgacc gccggcgaag tgacaggtgc
    45001 tacattgcct ttatgctggc atggcagtaa caagccgtta aacagatcgg caggcagcgg
    45061 gtccggcagg ttgatatcgc aatgcgcaac accactccag aacactctca tgtgttcgcc
    45121 aggtttcacc ccagccagat aaacattgcc gtcatcatcg acaaggccca ctgtctgctc
    45181 gttatcattt tttacttctg cgccaaacgg aggatggctg ccgtcttgta aacgcagcac
    45241 cgccatcgct ttttgaccac tgatgacggc aaatttgcgg tagccaatcg ccccttcagt
    45301 tagcgtggct tgtaccaccg actgggttgc ttcggcgttt tccggcagtt tgttgaggtc
    45361 gatatacgcc tgattgcggt aatagttatt gacgtcagaa acgacggctt taccaaacat
    45421 attggtataa acagcagcac cgttaccttc taccggaaca tcggcaacgc catcggcatc
    45481 aatcaacaag cgtgtcccgc ccatattctg ggtacggtga agtgcgccac cgtgggcagt
    45541 aagcgttgcg ccgccctgta acgacaagcc cgcagaggtg tattgccctt catggtagtt
    45601 cgcacagagg tcaacctgcg ccagcgaacc atcatggctg taatagccgt caacgctggt
    45661 gtgtttgtca ctggtgccga cgttcaactg atagtgagtc gcgtcatcga cacggctgaa
    45721 ataaccgacc tgactgctgt ccgtcccact gccatagtta ccgttatagc taacggtact
    45781 gttgtcgccc cacggcatac tgagcgaaat gtacatgccc ttatccgccc ggttgtcata
    45841 ctcgtagcgg tagccagtca gggaaacgct catattacga atgctgccca tattgaaata
    45901 gtgggagagc atgatgttgt agtttgtctg ctcctcacga tcccagtagg tatggcgggt
    45961 atagttgaga taaaccgaaa cacccgcatc gcggaagttc tggttataag tggcggtgta
    46021 catctctttg tcgttgcccg tgcggaccat ttcgctgtca ctggcatcca gatactcgct
    46081 catggtcata aagttctctt ccgagaagcg atatccagcg aaagtgacgc ggctgttgag
    46141 ctggtcaaag tctttggaat aactcacacg gaaggagtta ccgtccagcg aacctttgcc
    46201 ataagcggtg tctttatcca gtttggtatg cgaatgagta acatcaaacg cgaccgcgcc
    46261 gaaggtagac aaatcgcgac cgacgcccag cgccgcagac tgatagtttt catcacccag
    46321 cgcaccgcca tataacgacc agccgttagc aatcccccag gaagcttcag caccagagaa
    46381 aaattcgcct tcaacatgat gcccccactc ttgcggacgg cccatcatga tcttatagcg
    46441 aacctgacct ggacgagtga ggtatggcat cgaggcggtg ctgatgtcat attcctgcac
    46501 ctggccgttc tgttcttcaa tgcgaatatg caacgtaccg gagacagaat caccaagatc
    46561 ctgaatacga aacggtccag ccggaacctg cgtttcgtaa atcacacgcc ccatctggct
    46621 gacggtcact tttgctgtgg tgtgtgccac gccggaaatg tctggcgcgt agccgcgcag
    46681 attgggaggc aacatttgat cgtcggtgct aacgctgcca ccaacatagt taaagccatc
    46741 aaaaatatcg gatctgaggt aatcctcgcc cagcgccagt ttggctttta atgatggtaa
    46801 cgcccgccag gcatagtagc gactccactc ccattttttt tgagtttcat cgccgctgaa
    46861 ttcatcgtca tcatcattac tgcgagtatg ttgatagtta gtctgccagt cagcacgcat
    46921 acgccacggc cccaggttaa ccccgaccgt cccgttgccg ctgatctcgt tactatcatc
    46981 accgccattt tcttcgtgcc gggtttgtgc gttgatgctg tagtccgcga cgatcccgga
    47041 gatgccgtca tcccaacgtg aaggcggatc ccagtcgggc caggtatatt cgaggtaagc
    47101 ctgcggtagt gaaatgacta atgcggactg gcttaaatca gccttaattt ccatgccttc
    47161 cagttgaccg gatttcaggc atttagcatc gtggctccat tgcagatttt tcgccacgtc
    47221 ttcttttaaa ccaaactgcg ctaccagttc cggtgtcaga caagcatagc ttttgctcgc
    47281 gtcatcttca ccagcatacc agtaaatatc gtactcttcc gccaatggct gtttatttag
    47341 ttgaacctgt aaattgtatt ttccgggctc aacatatccc tggctggaaa aacgcttcag
    47401 atcaattttt gtgtcgcctt ttaattccag aaaacgggaa tcaaactgaa tgtcgtcttc
    47461 agcccagaca ctgctataac tccctgacat tgccagcgca atgcaccacg gaagtatccg
    47521 taatcgaaaa agagaatggt caggcataca cgtttctatc cgtttttatt cttattcggt
    47581 caaaatccat ttaaatcccc ccatagcggt gtcgctatgg ggggatgcct tacaggtaag
    47641 taatctggaa ggtggtattt gcttcgaaat tacctaagtc tggcgcatca gctgcgccga
    47701 ccagccaggc tttaaagttc agtgtctgct tcgctttgcc tttattagtg gcagtgtctt
    47761 tcacgatttt ctggtcgata cccatgccac ttttgtaaga ggtccccaga gagtcaccga
    47821 ttgccaggct aacgttgtta aatgccgcac cggtatcggt attgaaaatg gtgtaataat
    47881 tgccgctatt tgtagaagaa acggtgccgg tgaaggtagt ggtcattgtt tcctgagtat
    47941 caaatacgca atcttgcagg cgaatctgga aacttttcgg cgtggtggtg ccgttatttt
    48001 tcagtttttc ggcaccgata tcacccatat caacttccag tttgtgatcg tccggaatga
    48061 tagaacacac tgagtcttca atggtgccgt aaaaacgtgc agtaccgttg ttacccgcag
    48121 cgattgttgc cgtagaggtg aacgcgccca tcaccattgc cgcagcaata gctgttttaa
    48181 caaac
//
LOCUS       DAPHBJ010000010        46803 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.010, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000010 DAPHBJ010000000
VERSION     DAPHBJ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.010"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(329..871)
                     /locus_tag="RHZ76_000556"
     CDS             complement(329..871)
                     /locus_tag="RHZ76_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV2811249.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(894..1919)
                     /gene="nagZ"
                     /locus_tag="RHZ76_000557"
     CDS             complement(894..1919)
                     /gene="nagZ"
                     /locus_tag="RHZ76_000557"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HDV2811250.1"
                     /translation="MGPVILDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEKGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(1930..2754)
                     /gene="thiK"
                     /locus_tag="RHZ76_000558"
     CDS             complement(1930..2754)
                     /gene="thiK"
                     /locus_tag="RHZ76_000558"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HDV2811251.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENAEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2735..3376)
                     /gene="lpoB"
                     /locus_tag="RHZ76_000559"
     CDS             complement(2735..3376)
                     /gene="lpoB"
                     /locus_tag="RHZ76_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HDV2811252.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3390..3767)
                     /locus_tag="RHZ76_000560"
     CDS             complement(3390..3767)
                     /locus_tag="RHZ76_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HDV2811253.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3770..4129)
                     /gene="hinT"
                     /locus_tag="RHZ76_000561"
     CDS             complement(3770..4129)
                     /gene="hinT"
                     /locus_tag="RHZ76_000561"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HDV2811254.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4463..6652
                     /gene="fhuE"
                     /locus_tag="RHZ76_000562"
     CDS             4463..6652
                     /gene="fhuE"
                     /locus_tag="RHZ76_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HDV2811255.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTNTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGTPRNFSITSTYQF"
     gene            complement(6712..8145)
                     /gene="ptsG"
                     /locus_tag="RHZ76_000563"
     CDS             complement(6712..8145)
                     /gene="ptsG"
                     /locus_tag="RHZ76_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HDV2811256.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8440..9237)
                     /locus_tag="RHZ76_000564"
     CDS             complement(8440..9237)
                     /locus_tag="RHZ76_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV2811257.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9248..10252)
                     /gene="holB"
                     /locus_tag="RHZ76_000565"
     CDS             complement(9248..10252)
                     /gene="holB"
                     /locus_tag="RHZ76_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDV2811258.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10249..10890)
                     /locus_tag="RHZ76_000566"
     CDS             complement(10249..10890)
                     /locus_tag="RHZ76_000566"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDV2811259.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAKDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(10880..11902)
                     /gene="yceG"
                     /locus_tag="RHZ76_000567"
     CDS             complement(10880..11902)
                     /gene="yceG"
                     /locus_tag="RHZ76_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HDV2811260.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11905..12714)
                     /gene="pabC"
                     /locus_tag="RHZ76_000568"
     CDS             complement(11905..12714)
                     /gene="pabC"
                     /locus_tag="RHZ76_000568"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HDV2811261.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12834..14075)
                     /gene="fabF"
                     /locus_tag="RHZ76_000569"
     CDS             complement(12834..14075)
                     /gene="fabF"
                     /locus_tag="RHZ76_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDV2811262.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14163..14399)
                     /gene="acpP"
                     /locus_tag="RHZ76_000570"
     CDS             complement(14163..14399)
                     /gene="acpP"
                     /locus_tag="RHZ76_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDV2811263.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14610..15344)
                     /gene="fabG"
                     /locus_tag="RHZ76_000571"
     CDS             complement(14610..15344)
                     /gene="fabG"
                     /locus_tag="RHZ76_000571"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDV2811264.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15357..16286)
                     /gene="fabD"
                     /locus_tag="RHZ76_000572"
     CDS             complement(15357..16286)
                     /gene="fabD"
                     /locus_tag="RHZ76_000572"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDV2811265.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16302..17255)
                     /gene="fabH"
                     /locus_tag="RHZ76_000573"
     CDS             complement(16302..17255)
                     /gene="fabH"
                     /locus_tag="RHZ76_000573"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HDV2811266.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17323..18393)
                     /gene="plsX"
                     /locus_tag="RHZ76_000574"
     CDS             complement(17323..18393)
                     /gene="plsX"
                     /locus_tag="RHZ76_000574"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDV2811267.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18474..18647)
                     /gene="rpmF"
                     /locus_tag="RHZ76_000575"
     CDS             complement(18474..18647)
                     /gene="rpmF"
                     /locus_tag="RHZ76_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDV2811268.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18699..19220)
                     /gene="yceD"
                     /locus_tag="RHZ76_000576"
     CDS             complement(18699..19220)
                     /gene="yceD"
                     /locus_tag="RHZ76_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HDV2811269.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            19419..20003
                     /gene="yceF"
                     /locus_tag="RHZ76_000577"
     CDS             19419..20003
                     /gene="yceF"
                     /locus_tag="RHZ76_000577"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HDV2811270.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20115..21074)
                     /gene="rluC"
                     /locus_tag="RHZ76_000578"
     CDS             complement(20115..21074)
                     /gene="rluC"
                     /locus_tag="RHZ76_000578"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HDV2811271.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            21647..24832
                     /gene="rne"
                     /locus_tag="RHZ76_000579"
     CDS             21647..24832
                     /gene="rne"
                     /locus_tag="RHZ76_000579"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HDV2811272.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVHVQPMVTEVPVAAAIEPVVSAPVVEEVAGVVEAPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEQAEPVVEPQEETADIEEVVETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(25028..25981)
                     /gene="flgL"
                     /locus_tag="RHZ76_000580"
     CDS             complement(25028..25981)
                     /gene="flgL"
                     /locus_tag="RHZ76_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDV2811273.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(25993..27636)
                     /gene="flgK"
                     /locus_tag="RHZ76_000581"
     CDS             complement(25993..27636)
                     /gene="flgK"
                     /locus_tag="RHZ76_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDV2811274.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDEGEDFFAIGKPA
                     VLQNTKNNGNVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(27702..28643)
                     /gene="flgJ"
                     /locus_tag="RHZ76_000582"
     CDS             complement(27702..28643)
                     /gene="flgJ"
                     /locus_tag="RHZ76_000582"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HDV2811275.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(28643..29740)
                     /gene="flgI"
                     /locus_tag="RHZ76_000583"
     CDS             complement(28643..29740)
                     /gene="flgI"
                     /locus_tag="RHZ76_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HDV2811276.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMHVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(29752..30450)
                     /gene="flgH"
                     /locus_tag="RHZ76_000584"
     CDS             complement(29752..30450)
                     /gene="flgH"
                     /locus_tag="RHZ76_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HDV2811277.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(30503..31285)
                     /gene="flgG"
                     /locus_tag="RHZ76_000585"
     CDS             complement(30503..31285)
                     /gene="flgG"
                     /locus_tag="RHZ76_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HDV2811278.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGYKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(31457..32212)
                     /gene="flgF"
                     /locus_tag="RHZ76_000586"
     CDS             complement(31457..32212)
                     /gene="flgF"
                     /locus_tag="RHZ76_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HDV2811279.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(32232..33440)
                     /gene="flgE"
                     /locus_tag="RHZ76_000587"
     CDS             complement(32232..33440)
                     /gene="flgE"
                     /locus_tag="RHZ76_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HDV2811280.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLKFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQD
                     QILNTLVNLR"
     gene            complement(33465..34160)
                     /gene="flgD"
                     /locus_tag="RHZ76_000588"
     CDS             complement(33465..34160)
                     /gene="flgD"
                     /locus_tag="RHZ76_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HDV2811281.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34172..34576)
                     /gene="flgC"
                     /locus_tag="RHZ76_000589"
     CDS             complement(34172..34576)
                     /gene="flgC"
                     /locus_tag="RHZ76_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDV2811282.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(34580..34996)
                     /gene="flgB"
                     /locus_tag="RHZ76_000590"
     CDS             complement(34580..34996)
                     /gene="flgB"
                     /locus_tag="RHZ76_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDV2811283.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35152..35811
                     /gene="flgA"
                     /locus_tag="RHZ76_000591"
     CDS             35152..35811
                     /gene="flgA"
                     /locus_tag="RHZ76_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HDV2811284.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIARGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            35887..36180
                     /gene="flgM"
                     /locus_tag="RHZ76_000592"
     CDS             35887..36180
                     /gene="flgM"
                     /locus_tag="RHZ76_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HDV2811285.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            36185..36601
                     /gene="flgN"
                     /locus_tag="RHZ76_000593"
     CDS             36185..36601
                     /gene="flgN"
                     /locus_tag="RHZ76_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HDV2811286.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(36641..38176)
                     /gene="murJ"
                     /locus_tag="RHZ76_000594"
     CDS             complement(36641..38176)
                     /gene="murJ"
                     /locus_tag="RHZ76_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDV2811287.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(38286..39209)
                     /locus_tag="RHZ76_000595"
     CDS             complement(38286..39209)
                     /locus_tag="RHZ76_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV2811288.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASMDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVRAQRIVDKIWRDAMSE"
     gene            complement(39211..39858)
                     /locus_tag="RHZ76_000596"
     CDS             complement(39211..39858)
                     /locus_tag="RHZ76_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HDV2811289.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(39869..40453)
                     /gene="rimJ"
                     /locus_tag="RHZ76_000597"
     CDS             complement(39869..40453)
                     /gene="rimJ"
                     /locus_tag="RHZ76_000597"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HDV2811290.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            40689..41897
                     /gene="mdtH"
                     /locus_tag="RHZ76_000598"
     CDS             40689..41897
                     /gene="mdtH"
                     /locus_tag="RHZ76_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HDV2811291.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFNLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            41961..42608
                     /gene="grxB"
                     /locus_tag="RHZ76_000599"
     CDS             41961..42608
                     /gene="grxB"
                     /locus_tag="RHZ76_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HDV2811292.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            42742..43302
                     /locus_tag="RHZ76_000600"
     CDS             42742..43302
                     /locus_tag="RHZ76_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV2811293.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            43408..44454
                     /gene="pyrC"
                     /locus_tag="RHZ76_000601"
     CDS             43408..44454
                     /gene="pyrC"
                     /locus_tag="RHZ76_000601"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDV2811294.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            44528..44773
                     /gene="dinI"
                     /locus_tag="RHZ76_000602"
     CDS             44528..44773
                     /gene="dinI"
                     /locus_tag="RHZ76_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HDV2811295.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            45063..45317
                     /gene="bssS"
                     /locus_tag="RHZ76_000603"
     CDS             45063..45317
                     /gene="bssS"
                     /locus_tag="RHZ76_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HDV2811296.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            45432..46550
                     /gene="solA"
                     /locus_tag="RHZ76_000604"
     CDS             45432..46550
                     /gene="solA"
                     /locus_tag="RHZ76_000604"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HDV2811297.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            46598..46711
                     /locus_tag="RHZ76_000605"
     CDS             46598..46711
                     /locus_tag="RHZ76_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HDV2811298.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT        11339 a        12587 c        11623 g        11254 t
ORIGIN      
        1 ttacagcaat gaaaatgttc ttcgctattg agaataacat gaatggtgca ttggtcatac
       61 caaaattgat gttaatcaag ttttgttgct gaaaagatag gcgactttac cgggtgagat
      121 caaaaaacag agtttattgt ttattatttg agcgaatttg tgagagtgat atagagtgag
      181 gggtgtatgc agggcgttcg gtaggcctga taagaggcgc atcaggcatt gatgctcgac
      241 tgtcggatgc ggcgtaaacg ccttatccga cttacagaat tcggagcgca tcaggtaccg
      301 gcaaggttac ctgatgcgac gagggcattt acccgagggt tttgaacgct ttaatgcgct
      361 gcaaatgcgg ggagatattc ttgaatttgt gcgtctgctc ttcgtcccag acaatctcgt
      421 aataatgatg caactcttca gatgtccgct ggctgttaag cgcttcatca ttacgcgaca
      481 aaatcaccag gcaacgatcg cgattcttct cgcggaagtt ggtcacacac ttagtggcaa
      541 tatcggcata ctcttccggg cgatcaatct tgccttccat gttctcataa gggaacaaat
      601 tagggttgaa gatcacctgg cggatatcgc aaagaaaacc aatccgttcc gcccagtatc
      661 cgcccagacc aacgccgcaa atcagcggac gctcgtcgac gttcagttgc aacattttgt
      721 ccacttcttt gagcagatgc tgcatatcat gtttcggatg ccgcgtactg tagcttatca
      781 agcgtacatc cgggtcaata aactgcaatt gtaagacttt ctcgtggtta cccggactgt
      841 tagagtcaaa accgtgtaaa tagataatca tcgcttcctc acataagcca gggttagtga
      901 cctgctttct cttcctgcca gcgttcatgt aactgattca gacgggtgct gatcgccttc
      961 cagcgagccg agtccatcag ttcctgtcgc gaaaatgaac ctttatgata caaacgtgta
     1021 acacgttctg ccttgatcgg tgacagatta tctaacacgc tgacggcccc tttacgatta
     1081 ttgcagacca ggatcatatc gcaacctgcg tccagtgatg cctgcccgcg ttcggcataa
     1141 ctgcccataa tcgcggcacc ttccatcgat aaatcgtcag agaaaatcac gccgtcaaaa
     1201 cacagttcct gacgcaaaac ggttttcagc cagtaggaag aaccgctcgc cggacgcgga
     1261 tcaacatcac tgtagatcac atgcgcaggc ataatggcgt cgagtttatt ttcgcggatt
     1321 aacgtgctga aaaccgacat atctttggca cgaatttccg cttgcgggcg tgggtcgcac
     1381 ggcgtctctt tgtgtgaatc tgcagttact gcaccgtgtc ctgggaagtg tttcccggtc
     1441 gttttcattc cggcttcatg catcccatca ataaaccgac tggcgattgc cagggctttt
     1501 tctgggtcgg catgataaga acgctcgcca atcgccgcgc tgatatgtcc tacatccagc
     1561 actggcgcaa agctgatatc aatatccata gcgatcattt cgctggccat cagccaaccc
     1621 gcctcttgcg ccagtttgcc gcccttttcc attcccaaca gcgcagcaaa ggattgtgct
     1681 gccggtaagc gggtaaaacc ttcgcgaaaa cgctggacgc gtccaccttc ctgatctacc
     1741 gccaccacca gatgattgcg cgatgctgcg cggatctggc gcaccagttc acgcaactgg
     1801 gcaggatcat gatagttacg cgtaaagaga atcagccctc ccaccagcgg atgcgcaagt
     1861 atttcacgct cttccgcgtc cagttcgtaa ccttcgacat ccaatattac tggacccaca
     1921 ctgctctcct tattgtttta ttaatagctg ccgccaggtg tcatcggcca gcctgataaa
     1981 ttgttgatcg ccggtttgtc gccagcggta ctcaaaccac cctgctttga gcatcagcag
     2041 ccagggaaac catcgcctga cctgacgcca taattgcgcc ggataaatct tcgcgcgagt
     2101 ggcatagtca ttgaccaatt gccggtgctg ttcagcattt tccacccaca ccgccgccag
     2161 ttccagcgcg atatcaccat ctccggcata ctcccagtcg atgagtttta accctgacgc
     2221 gctatgcact aaatttccgg cgtggacatc catatgcaat ggacttaagc gtaaaggccg
     2281 tggttcccgc gctttgcgca gacgttttaa cattcgcagc caacccactg tccgccgcgc
     2341 cggatcgctt tgctgccagt acagttccag taacggcaac agcgttattc gccagccaaa
     2401 acgtggttgt tgatgtagat aatacagcaa gcctgccagt tcgttggtat ccggcaaata
     2461 cgtttttacc gcgccgggca gatagtcgac taccatccag tcacggagat ataaatgcgg
     2521 cttcggtgca atgcttgcgg gtagttgtga taaagcccga tactggcgca agaacgcgga
     2581 ctgcggcgca tcaggatcgt gcggctgacg cacaacaaaa cgctggccct gatgttcaat
     2641 gagaaaactc ccgccactaa gcccactatt aaacgtcgtg acgggatgaa actgcgggaa
     2701 aaagcgcgac agcaattcgt cgcgcatgat gggattattg ctgcgaaacg gcacctttac
     2761 ctgaccagat aatttcgccc gtctgcacca gcatcagctg catttgtagg gtcggagcgt
     2821 taacgttgcc agaggcgctg gagtacagca cgtaatgagc gccgacattg cgggcaatgc
     2881 ctatggcttt actacgggta cctaaactgt cctgcggcga caaacctaac tgttgcttag
     2941 ccatcgacag ctgctgggcg gaaaccaggg taaatttccc gttattagcc agcgcatttc
     3001 gcagcgtttc ggtcgcttct gcggcattca gcgaaccgtt agtacggttg ttaacgctat
     3061 caaccagcag gacgctaccc gcagtcaccc cgtcagcccc aagcatctta ctgaccatcg
     3121 gctgcattgc gccattccag tcataatggc gaatatgcgg cgcaggcggt gccgtttgat
     3181 cttcgtgctc aattgggcct ggctgctgcg ggatcgtcgg caccgagggc actgtgggga
     3241 caggctgttg tggctcggct ggttgttccg gcgctggttt cacttcttct accggtgcag
     3301 gttcacgttg ccccacacac ccggcgagaa acatcgccag cgcggtaatc aaggcgtagc
     3361 gactcatttt tgtcatcaag attcacccct tacaaatata gataaagtct gactttgtgc
     3421 gcccccagaa aattggcgct gccgtacagc gttaccgccg aatgtgcggg aatgctaacg
     3481 ctgcgtggcc tttccagagg atgcatctcc agccctctgg cgtcatacca gtaaaaacga
     3541 taatgaacgg taacgggttc ttgcctttcg ttataaagcg ttgaggatgc tgaaggttga
     3601 atatcagacg tcgaaaggac gggcttttct gcactgatgc ccgcagccaa taaagatgac
     3661 tccatcacca gcgattgctc atcattcacc ggaatttccg gatgtgaacg acagcccacc
     3721 agcagtaaca acgccagaga caccagccca aagcatcctt ttctcatcgt tacagacctt
     3781 tatgcgccag cattggtccc agcggacggc cacccaacaa gtgcatatgg atgtggtaaa
     3841 cctcttgtcc gccatggcgg ttggtattca tgatcagacg atagccatct tcggcaatac
     3901 cttcttgctc agcaattttt gccgctacgg tgatcatgcg tcccagcgcc tgctcatgct
     3961 cagctgagac gtcgttcaca gtcggaatga ggatattcgg aatgatcaga atatgcgttg
     4021 gcgcttgcgg cgaaatatcg cgaaacgccg ttaccagatc atcctggtag acgatatcgg
     4081 aggggatctc acgacgaata attttgctga atatagtttc ttctgccacg acattttcct
     4141 ttttcaaata gccatgcgtt gcgccatgct acgccgcgcc acactgcgag tatagagtat
     4201 ggtcgagtta aacctgctct ttcaacctta actcacgatt tcttaagcaa aaaatcgttt
     4261 tgctgccgta tatatcgcca ttattcccat ttctgcctgc gaatgcgctg aaaagacatt
     4321 ctctgtttgg tttacatgac aaatatgaat gcgtatattt ctcatttgca tttacaaaca
     4381 aaattattcg cacactaaat ataatcgctc atcaccctcc tccggcatga gcctgacgac
     4441 aacataaacc aagagatttc aaatgctttc aacacaattt aacagggata atcaatatca
     4501 agccatcacc aaaccgtcac tacttgccgg ttgcatagca ctggcactat taccttctgc
     4561 cgcttttgct gcaccagcca ctgaagaaac ggtgattgtt gagggttcag ccacagctcc
     4621 agatgatggc gaaaatgatt acagcgtaac gtctacctct gcgggtacca aaatgcagat
     4681 gactcaacgt gatattcctc agtcggtcac tattgttagc cagcagcgga tggaagatca
     4741 gcagttacaa acgctgggcg aagtgatgga aaacacgctg gggatcagca aaagtcaggc
     4801 ggattccgat cgtgctcttt attattcccg cggattccag atcgataact atatggttga
     4861 tggtatcccc acctattttg aatcgcgctg gaatctgggc gacgcacttt ctgatatggc
     4921 actctttgaa cgcgtagaag tagtgcgtgg cgcgacagga ctcatgaccg ggacgggtaa
     4981 tccatctgcg gcaattaata tggttcgaaa acacgcgacc agtcgtgaat ttaaaggcga
     5041 tgtctcggcg gaatacggta gctggaacaa agaacggtat gtggcggatt tacaaagccc
     5101 actcaccgaa gacggtaaaa tccgcgcgcg aattgtcggc ggctaccaga ataacgactc
     5161 atggctggac cgctacaaca gtgaaaagac cttcttctcg ggcattgtcg atgctgattt
     5221 aggcgatctt acgacgcttt cagccggtta cgaatatcag cgcattgatg ttaatagccc
     5281 tacctggggc ggtttaccgc gctggaatac tgatggcagc agcaacagtt acgatcgtgc
     5341 acgcagtacc gcacctgact gggcgtacaa cgataaagag atcaacaagg tctttatgac
     5401 cctgaagcag cggtttgctg atacctggca agcgacactg aatgccaccc actctgaagt
     5461 cgaatttgac agcaaaatga tgtatgtcga tgcctatgta aacaaagcgg atggtatgct
     5521 ggttgggcca tacagtaatt atggacctgg ctttgattat gtcggcggca ccggttggaa
     5581 cagtggcaaa cgtaaagttg atgcgctgga tttgttcgct gacggtagtt atgaattgtt
     5641 tggtcgtcag cacaatctaa tgtttggtgg cagttacagc aaacaaaaca atcgttactt
     5701 cagttcatgg gccaacatct tcccggatga aattggcagt ttctacaact ttaatggcaa
     5761 tttcccacaa accgactggt caccacagag cctggcgcag gacgatacca cacatatgaa
     5821 atcgttatat gctgccactc gtgtcaccct tgccgatccg ctgcatctga tcctcggcgc
     5881 acgttatacc aactggcggg ttgatacgct gacttacagc atggagaaaa accacaccac
     5941 gccttacgct ggtctggtgt ttgacatcaa tgacaactgg tcgacctacg ccagctatac
     6001 ctctattttc cagccgcaaa atgatcgtga cagttcaggc aaatatctgg ctccaatcac
     6061 cggtaacaac tacgagctgg gtctgaaatc ggactggatg aatagccgtc tgaccaccac
     6121 gttagccatc ttccgtattg agcaggataa tgtcgctcag tccaccggta cacctatccc
     6181 cggcagcaac ggcgaaaccg cctataaagc ggtggatggg acagtcagta aaggggtgga
     6241 atttgaactc aacggcgcaa ttaccgacaa ctggcagctg acatttggcg caacgcgcta
     6301 tattgcagag gataacgaag gaaacgccgt taatcctaat ctgccacgca ccacggttaa
     6361 aatgttcacc agctatcggt tgcctgtcat gccagagttg acggtcggcg gtggtgttaa
     6421 ctggcaaaat cgcgtgtata ccaacaccgt gacaccgtat ggcaccttcc gcgccgagca
     6481 aggtagctac gcgctggtgg atctcttcac ccgctaccag gtgacgaaaa acttctcgtt
     6541 acaggggaac gtcaataacc tgttcgacaa aacctacgat accaacgtgg aaggttctat
     6601 cgtctacggc acaccgcgta atttcagcat taccagcacg tatcaattct gaataacacc
     6661 tgtaaaaaag gcagccatct ggctgcctta gtctccccaa cgtcttacgg attagtggtt
     6721 acggatgtac tcatccatct cggttttcag gttatcggat ttagtaccga aaatcgcctg
     6781 aacaccagaa ccagcaacca ctacgcccgc tgcgcccagt ttcttcaggc cggcctgatc
     6841 cactttagac acatcagcaa cgctgacgcg cagacgggta atacatgcgt cgaggttagt
     6901 aatgttttct ttaccaccaa atgcagcaac cagagccggt gccatttcgc tggtacctgt
     6961 cgcttttgca tcttcagtcg cgtcttcacg acccggcgtt ttcagatcca gtgctttaat
     7021 cagcacgcgg aagatggtgt agtaaacaat cgcataaccg ataccgacga tcgggaacag
     7081 ccacagtttg ctgctgttac cagacagaac gatgaagtcg atcagaccgt gcgagaacga
     7141 cgtaccgtca cgcatcccca gaagaataca gattgggaat gccaggcctg ccagaatcgc
     7201 gtggatgatg tacaggatcg gcgcaacgaa catgaaggag aactcgatcg gctcggtgat
     7261 accggtcagg aacgaggtca gcgccgcgga gatcataata ccgcccactt tcgcgcggtt
     7321 ttctggttta gcagagtgcc agatagcaat tgcggcagct ggcagaccgt acattttgaa
     7381 caggaagcca ccagacagtt tacccgcagt cgggtcaccc gccatataac gcggaatgtc
     7441 gccgtggaaa acctgacctg ctgcgttggt gtattcacca atctgcatct ggaaaggtac
     7501 gttccagatg tggtgcagac caaacggtac caggcaacgt tcgatgaaac cgtaaatgcc
     7561 aaacgcaact accgggttct ggtaagcagc ccactgagag aaggtctgga ttgcagaacc
     7621 aatcggcggc caaatgaagg acagcacaac gccagtaaag atggcagcca ggccagaaat
     7681 gatcggcaca aagcgtttac cggcaaagaa gccaagatac tcaggcagct taatacggta
     7741 gaaacggtta aacatgtacg ctgcgatcgc accggagata atccctccga gtacgccagt
     7801 atccgccagg tgtttagagg cgatttcttc agcaggtaaa tgcagtacca gtggcgcaac
     7861 cacggccatg gttttaacca tgatgccata ggcaacaact gcggccagcg cggatacgcc
     7921 atcgttattg gtaaagccga gggcgacacc gatcgcaaaa atcagtggca tgtttgcaaa
     7981 gacggaaccg cctgcttctg ccataacatg cgatacaacg gcgggcagcc agctgaaatt
     8041 cgcggaaccg acgcccagca gaatacctgc gataggcagt acggataccg gcagcatcag
     8101 cgatttaccg accttttgca ggttagcaaa tgcattctta aacataattg agagtgctcc
     8161 tgagtatggg tgcttttttt acgttctcac gcgtggcaag gggggagagc ctcgccgtgt
     8221 acagggcatc taagcgccct ttatttatta cacagagtaa aataattcag tgccaatttg
     8281 tttgacggct atcacgtttc aacttcagat cattggagaa gttgcacatt ttgacaaaac
     8341 atatgtcaca atatttcaaa cgtggttatt caccgcccaa ttttcggcgg tgaactttac
     8401 tcgttttagc cattaattac gagctttaaa aaaactcatt caacggatgg attgaaggcg
     8461 ggaagcgtcg atgtgaaaca gacgggcgaa gttatcggtg gttacctgcg ccagttcttc
     8521 aacggcaaca cctttcaaca cagccatgta ttctgcaacg tcacgaacca tcgcaggttg
     8581 attctctttt cctcgatgcg gtaccggcgc aaggtaaggt gagtcagttt ccaccagtaa
     8641 ccgatccagg gggacataac gcgcagcatc gcgcagttgc tccgcattac ggaaggtcac
     8701 aatgccggaa aaggagatgt aaaatccgag atccagtaat ttacccgccg tttctctgtc
     8761 ctctgtaaaa cagtgtagta cgccaccgca atccgtcact ttttcttcgc gcagaattgc
     8821 cagcgtatcg gcacgggcgt cacgggtatg gacgataacc ggcttgttca gttcacgacc
     8881 aatctggata tgatggatga aggactcttg ctgacgtact ttagtttccg gtgtgtaata
     8941 ataatccagc ccggtttcac ccagcgctac aacaccctct tctgccgcca gacggcgtaa
     9001 atcttctaca tcgtagggat cattctggtt gagtggatgc acgccacaag aaaatacgac
     9061 gttgtcacgt tcgcctacca gatcccgcat atgtaaataa cccggtaatg ttgtggcgac
     9121 tgccagacaa aatttcacat cgcgtgcggc ggctttcgcc agaacgtcat ccacgtcctt
     9181 atgcaaagat tcataatcca gaccatcgag atggcagtgt gagtcgacta aaaacatgat
     9241 gtctctctta aagatgagga accggtagca caacgcccgg ttgcaggtaa tgctcaatac
     9301 gcagtaaaag atcggtgatg agaagctcgc ggttgatgcc tgtgacagac attaactgtt
     9361 cacgaatgtg gcaaacatcc cccagtatag cctgcaggcg cgagggagaa agatggtttg
     9421 ccagttcggc gaccaggccc ggcacatcaa cattggtcac ctgcgcagcg ccatgatggc
     9481 gttttagcgc atccatcagc aacgttgcca gccagtgtaa acgcgccgga gcttgttcat
     9541 gattaagggc cgccagcagc gaataccagt cgcccgatgg cacgctatat gccaacgcct
     9601 gacacaatgt ttcacgagcc tgccagttat ctccctgaaa caacgccagt gccgcgccag
     9661 gcgaaccggc gcttaagcgc aatgcggcaa gtaatgcatc ctgtgacatt gtcacttcgc
     9721 gtgaaagcca ggtcacggcg tactgttctg gaggcggcgc aaggtaatgt aaccgacaac
     9781 gactacgtaa tgttgccagt aaacgttcag gctcgcgggt agccaggaaa aaccaggttt
     9841 ctgctggtgg ctcttcaagc gttttcagca atgcgttagc cgcggcgtcg gttagtaagg
     9901 cagcatcggt tacccagaca actttcgcac cacctaagcg tgcgtgctca ttcagctttt
     9961 cggtgacctc acgtaccgca tcaatgccca acgcattttt ccctttttcg ggagccaggg
    10021 tgtagtaatc gggatgcgtg ccagcctgca tcaactgaca tccacgacag tgaccgcaac
    10081 ttttgtggcc ctgcggttgt tggcagagta aataacggct cagggcgtag attaaagcat
    10141 catcgcccat gcccggtaac gcctgaatga gcagcgcatg gtgacctctt ccggcctgat
    10201 agctggctac cagtttttcg aaatcaggtc gtaaccatgg ataccatctc atgcgtccaa
    10261 ctccttcacc cagtgggtca cggtagtgcg gattgcatcc atcacggcct ccagcggctg
    10321 ggtggcatca atggtatgaa tgcttttatc ttttgctgcc agttccagat agcgggcgcg
    10381 ggtgcgatta aagaaatcga aagactcttg ctcaatacga tccagctcgc cgcgcgcacg
    10441 cgcgcgtttt aagccaactt ccggggtaac atcgagatag agcgttaagt cggggcgaaa
    10501 atcaccgaga acagcatcac gcagtgttgc cagcatatgt tggtcaatac cacgtccgcc
    10561 gccctgatac gcctgagtgg agagatcgtg gcgatcgcca atcacccagg tgccgttagc
    10621 cagagctggt ttgatgactg tttctaccag ttgaacgcgc gcggcataaa acatcagaac
    10681 ttcggcttta tcggtaatga cttcatcgcc taccgatttg atatccagca ccaggcttct
    10741 taacttttcg gcaagttgcg taccgccagg ttcccgagtg aaaaccatgt cgcggatacc
    10801 cagttgctcg agcgtctcaa ccaccacatt acgcgcggta gttttgcctg cgccttccag
    10861 cccctcaatg acgatatact tactgcgcat ttttttcctt aagcactttc agataatcct
    10921 gcacagactt gttatggctg gcaagattgg tattaaacgt gtgaccacct ttaccatcgg
    10981 ccacaaaata gagatacggc gtttttgccg gatgcgcagc agccttcagc gaatccgccc
    11041 ctggcgtagc tatcgcaccc ggcggtaaac cagtaatggt ataggtgtta tacgctgtcg
    11101 gcgtttccag gtctgcacga gaaagtttgc cattataacg ctctcccatc ccgtaaatca
    11161 cggttgggtc ggtctgcagg cgcataccaa tgcgtaaacg gttgataaat accgaggcaa
    11221 cctgatcgcg ttcactggca acggcggttt ctttttcgat aattgatgcc atcgtcacca
    11281 gctgattttt atctttataa ggcagaccgt ccgcacgccc ttcccaggcg ctatcgaccg
    11341 ctttcaccat tttcttgtgc gctcgcttga gtaacgcgac atcggttgta ttggcggtat
    11401 acatccaggt gtctggccag aaccaacctt caatccactc cgggttttcc agttcaagtg
    11461 cctgcgctac ggtggcgtac ttatcgtcgc tcagcgtatg cttgatatac ggggcctcac
    11521 gcaattgctt gaggtaatcg ctcagacgca tcccttctac cagtcgcaga gggaactgtg
    11581 cttctttacc gctttccagc aatttcagca tctcgcgcac ggtcatctgc ggtgtaaagc
    11641 ggtaagtccc ggctttaaag tgagaaagat ccggttcgat acgcagcagc cattgaaaaa
    11701 cccgtgggcg attgatgatc ttatcggcat aaagctgttc accgagcgcc agacgtccgg
    11761 tccctggctt cagggtaaat atcgtctctt ctttgataag caatttgctg tcggcaagat
    11821 ggcgaacctt ccagacgccc acaccagcgg cgatacccag taccaccagc aataacaaga
    11881 taatcaataa cacttttttc atgactaatt cgggcgctca caaagtgggg ctaaatattc
    11941 atataacgtt gctgacgaaa aggagacatc gccacaggca cgtacgggca tcactggcat
    12001 taacgcatta caaataacca tctcatctgc ctgcaacgcc tcttccagag aggcttgcac
    12061 ttcgacaagc tgataagagg attgtgccag caaacggata cagaattgtc gcataatgcc
    12121 gttaacacct gcctgatcca gtcgcggcgt ataaactacg ttgcccttcc gccagaacaa
    12181 attagccgca cagcattccg taacccaccc ttcgctgtca aggaccagcg cctcatcagc
    12241 gtttgtctgc tcaagatgag agcgaatcaa tacttgctca agacgattga gatgtttaat
    12301 acctgcaaga tgaggattgc gccccagccg caccgggctt agcgccaacg tcatcccctc
    12361 gttacgcaaa cggtcgtaat gtgcaggata agccgtaacg gagagaatcc gcgttgccgg
    12421 tccactgttc aatgtgctgt accctcgccc gccactaccg cgactgatca cgactttcag
    12481 tacaccattt tgctgttctg ctgccagcgt tttcatctct tgttcaagct gtggccagaa
    12541 gtcacaggaa atcatcaacc gctgacaagc atcctgtagt cgctggatat gcgccgataa
    12601 caaactgact ttaccgtcga taactctggc ggtggtaaaa caaccatcac caaactgcgt
    12661 tgcccgatcg cttactgcca gcgattcctg cttataaccg ttaattaaga acataccggc
    12721 tccttatcag agaattgtta gtgtggcagc atgttcacta cggaacaagt cggaataaaa
    12781 gctaagaaaa aaggcccgca agcggacctt ttataagggt ggaaaatgac aacttagatc
    12841 tttttaaaga tcaaagaacc attagtgcca ccgaagccga aggagttaca cagcgtgtat
    12901 tccattccgc tgacctgacg tgcttcatgc ggtacgaaat ccagatcgca accttcatcc
    12961 gggttatcca ggttgatggt tggcggaaca gcctgatcgc gcagcgccag gatggagtag
    13021 atagattcta ctgcacccgc cgcacctaac aggtgaccgg tcatagattt cgtggagctt
    13081 accaacacac ggcttgcagc ttcaccgaag atggttttca ccgcctgcgc ttcagcttta
    13141 tcgccagccg gcgtagaagt accgtgcgcg ttaacgtagc caatctgact cgcttcaatg
    13201 cctgcatcac gcagagcatt tgccattgcc agagctgcgc ctgcgccatt ttctggcggt
    13261 gacgtcatat gataagcatc gctgctcata ccaaagccga cgagttcagc gtaaattttc
    13321 gcaccgcgtt ttttcgcgtg ttcgtactct tcaagtacca gcataccggc accatcgccc
    13381 agtacgaaac catcacgctc tttatcccac gggcggctcg ccgcttgcgg gttatcattg
    13441 cgggtagata atgcacgtgc cgcgccaaaa ccaccaacac ccagcggcgt actggctttc
    13501 tctgcgccac ctgcaaccat cacgtcagca tcgccatacg cgataatacg cgcagcatgg
    13561 ccaatgttgt gcacgccgga agtacaggcg gtcgcgatag agatgcttgg gccacgcagg
    13621 ccatacatga tagtcagatg acctgccacc atgttcacaa tcgttgacgg aacgaagaat
    13681 gggctgatct tacgtggacc accgttcatc agagatgtgt ggttttcttc gatcagtccg
    13741 aggccgccaa tcccggagcc aattgcggca ccaatgcggg ttgcgttctc ttccgttatt
    13801 tcaaggccag aatcctgcat ggcctgaacg ccagcgacaa ttccatattg aatgaaggca
    13861 tccatcttgc gctgttcttt gcgcgagata atgtcctcac agttaaaatc ctttactaag
    13921 ccagcaaatt tcgttgcata ggcgctagta tcgaaatggt cgattaggct gatgccactc
    13981 tgaccggcaa gcagagcttt ccaggtagac tctacggtat tgccgacagg agacaacatg
    14041 cccagtccgg tcacaactac acgacgctta gacacgtttg tcctccaggg agggaaaaaa
    14101 tgattctagt gggacaaaaa gataaaactc aggcggtcga acgaccgcct ggagatgttc
    14161 acttacgcct ggtggccgtt gatgtaatca atggcagcct gaacggtggt gattttctca
    14221 gcttcttcgt ccggaatctc agtatcaaac tcttcttcca gagccattac cagctcaacg
    14281 gtgtcaagag aatccgcgcc caggtcttca acgaaagaag cattgttggt aacttcttcc
    14341 tgcttaacgc ccagctgttc gccgataatt ttcttaacgc gttcttcgat agtgctcata
    14401 ctcttaaatt tcctatcaaa actcgctttc gcgatggttt tcgtagtgta taaaatgttg
    14461 aaaaatttgc aactaaatcc cggcaggtct taccacgatt ttacgttatt ttgaggcctt
    14521 accccccaat aacgcaaatc attttgcact aattattact cataaccacg cagactgcgc
    14581 gcaacatgag cattttgtgc aaatcgcggt cagaccatgt acatcccgcc gttcacatgc
    14641 aaagtttcac ccgtgatgta agctgcttcg tcggatgcca ggaatgcaac cgcgttggcg
    14701 atttcctgtg cgccgccgag gcgacccgca ggaacctgcg ccaggatacc cgcacgctgg
    14761 tcatcgctca gcgcacgtgt catgtccgtt tcaataaagc ccggagcaac aacgtttaca
    14821 gtaataccgc gtgacgcaac ttcgcgcgcc agtgatttac tgaagccgat caagcccgct
    14881 ttcgccgcag cgtagttggc ctgaccgcca tttcccatgg taccaaccac agaaccgata
    14941 gtgataatac gaccatgacg ctttttcatc atagcgcgca ttaccgcttt tgacagacgg
    15001 aaaacagatg aaaggttggt ttcgataata tcgttccact cttcatcttt cattcgcatt
    15061 aacaggttat cacgagtgat accggcatta ttgaccagga tatccacttc accaaattct
    15121 gcgcgaattt tttccagaac agattcgata gatgccgggt cggtcacatt caacatcaga
    15181 cctttgccgt tggcacctaa ataatcactg atcgcctgag cgccattttc actggtcgca
    15241 gtgccaataa ctttcgcgcc acgggctgcg agcgtttcag caattgcgcg gccaattccg
    15301 cggcttgcac cggttaccag tgcgattttt ccttcaaaat tcatgatttt cctcttttaa
    15361 agctcgagcg ccgctgccat cgctgaaggt tcgttcagcg ccgaggcggt cagggtgtcg
    15421 acaatgcgtt tcgtcaggcc agtaagcact ttgcccgggc cgacttcata gagatgttct
    15481 acgccttgcg ctgccatgta ctcaacagac ttcgtccact gaaccgggtt atacaactga
    15541 cgtaccagtg cgtcacggat ggcatcacca ttggtttcgc atttcacatc aacgttattc
    15601 acaacaggaa ctgttggtgc gttaaaggtg attttcgcta attctactgc cagtttgtcg
    15661 gctgctggtt tcatcagcgc acagtgagac ggtacgctca ctggtaacgg cagagcgcgt
    15721 tttgcgcccg cagctttaca ggcagcgcca gcacgctcaa ccgcttcttt atgaccggca
    15781 ataaccacct gtcccggaga gttaaagttt accggagaaa cgacctgacc ttctgcagct
    15841 tcttcacacg ctttcgcaat agacgcatca tccagaccga tgattgccgc catagcgccc
    15901 gtgccttccg gtacggcttc ttgcataaac ttgccgcgca tctcaaccag acgcaccgca
    15961 tcagcgaaat caatcacacc agcgcaaacc agcgcggagt attcccccag gctgtgaccg
    16021 gccatcattg ccggtgcttt accgccctgc tgctgccata cgcgatacag cgcaacagag
    16081 gcagtcaaca gcgcaggctg agtttgccag gttttattca gttcttcagc tggcccctgc
    16141 tgggtcagcg cccacaggtc gtagcccagc gccgcagaag cttcagcaaa cgtttcttcg
    16201 acaattggat agctcgccgc catatcagcc agcattccaa cggtttgaga accctgtcca
    16261 gggaacacaa atgcaaattg cgtcatgttt taatccttat cctagaaacg aaccagcgcg
    16321 gagccccagg tgaatccacc gccaaaggct tcaagcagaa ccaactgccc cggcttaatg
    16381 cgcccgtcgc gtacagcttc atccagcgcg cacgggacag aggccgcaga ggtattaccg
    16441 tggcgatcca gcgtcaccac gacattgtcc atagacatac cgagtttttt cgccgttgca
    16501 ctgataatac gcaggttagc ctgatgcgga accagccagt ccagttgaga acggtcaaga
    16561 ttattcgccg ccagcgtctc atcaacgatg tgcgccagtt ccgttaccgc aaccttgaag
    16621 acttcgttgc ccgccatcgt cagatgaatt gaattctctg gattcacgcg gtcggcgttt
    16681 ggcagcgtca gcaattcacc ataactaccg tcggcatgca gatgggtgga aatgattccc
    16741 ggctcttcag aggcagccag caccgcagcg cccgcgccat cgccaaaaat aataatagtc
    16801 ccacgatctg ttggatcgca ggtgcgcgcc agtacatcgg aaccgacgac cagagcatac
    16861 ttcaccgccc cagatttcac gtattgatcg gctacgctta atgcataggt gaaacctgcg
    16921 caggctgctg caacgtcaaa tgccgggcaa cctttaatgc ccaacatgct ttgaatctga
    16981 caagctgcgc tcgggaaagc gtgcgtagca gaagtcgttg ccacaacgat caggccaatc
    17041 tggtctttct caatgcccgc catctcaatt gcgcgtgtcg ccgcttcaaa gcccatggtt
    17101 gaaacggttt cgtttggcgc ggcaatgtgg cgttcgcgga taccggtacg agtgacaatc
    17161 cactcgtcag aggtgtccac cattttttcc aaatcggcgt ttgtccgcac ttgttcgggc
    17221 agatagctgc cagtaccaat aatcttcgta tacatgtacg ctcagtcact tttcggttat
    17281 ataccgtcac ttgcaaactg cgagttcgct ggcagcgtcc tgctaccgca gagttccgct
    17341 tttgccaccg tccagcagct caaaaccagc tgggtataca gattccaggc gagcggcaat
    17401 tcgctgagga acttgtcgct gcaccgcctg cactgcctgt tcaatcgcga ccgcaaaagc
    17461 tcgctgattg gctgcaccat gactttttat caccgtgccg cgcaatccta acagacaggc
    17521 gccgttatac tggtcggggt tgaggtgact gaatcgcctc gtcaggctct tttgtagcca
    17581 acgctttaat aacagtagcc accacgaccg ttttttccct tcaccctgag atttcagcag
    17641 agaaaggaac atcctgacaa caccttccat cgtctttaat gtgacatttc ctgtaaagcc
    17701 gtcacaaacc agcacatctg tcttgccagt taacaactca ttggcttcaa gatagccgat
    17761 ataattgata gaagggattg ttttaagcac cgctgaggca tcccgaatac tgtcgagacc
    17821 ctttacttct tcttcaccaa tattgagcaa cgccacgcga ggattgggaa tttccaccac
    17881 ctcttcagcc agaactgagc ccataatggc aaattgcacc aacattgtac tgtcacaatc
    17941 gacgttggcc cctaagtcaa ggaccaccgt tttgcccttt tgctgatgtg gtaataccgt
    18001 caccagcgcc ggacgctcaa tcccctccag gggcttgagt aataattttg ccagccccat
    18061 cagtgccccg gtattaccgg cactgacaca ggcttgcgct cgaccttctt tcaccagctc
    18121 cagggccacg cgcattgaac tcccacgact ggcgcggata gcttgcgaag gccgggcatc
    18181 actggcgata actgactgcg caggaataat ctgcagacgc gaacgttgtt caaagtcagc
    18241 tttagcaagt aatggcgtga tggcgtcggg attgccgact aaaagaagag tgagttgcga
    18301 attagaattc agtgcctgca atgctgcagg cactgtcacg gaagggccaa aatcccctcc
    18361 catgacatct aacgccaggg ttagacgtgt caaggtatcg tcgccgcccg gtttggtctt
    18421 tccccagttg cctggggaaa atcctcacta agcttcatca cgcagacgcg tgattactta
    18481 gcgatgacct tgcggccgcg gtagtaaccg tcggcagtga tgtggtgacg caggtgtttt
    18541 tcaccagaag ttttgtctac agacaggctg gtgactgcgg tcagcgcgtc atgggaacga
    18601 cgcatgccac gtttggaacg ggttggttta ttctgttgta cggccatgga ccttactcct
    18661 caattacggt ttatccccga tccaacgggg agcaccaatt acttacgctt taagctggct
    18721 aatacggcaa atgggtttgg cttttgcgct tcttcaggca gttcaccaaa gaccatgtcc
    18781 gcttcggaca cttcacagtg ttcagaatca tgcaccggaa ctaccggcaa ggcgaggatg
    18841 atttcatctt caaccattgc aagcagatcg atttcaccga attcgttaac ctcaatcggt
    18901 tcatacgctt ccggcagtgc ttcagcctgt tcgtctgaac gcacaggact aaaacaatac
    18961 gttgtgtaga cctgatgagt aaacggcttc ccgcaacgct gacactcgag cgttaccgtc
    19021 accttcgcat cgccgtttaa cactgcgaga cgttggttat cgatagcgaa cgacatggag
    19081 cattccacat cactgtccac actgactacg gattcggcga cgcgctcaac ctgatcaggg
    19141 gtatagatac cctggtaatc aaggcgtttt tgagccgtac gaaccggatc gagagtcagg
    19201 ggtaatttta ccttttgcat agggcgcgca tattaacttt gtaacgtcat agagtcaaag
    19261 aaaaaggcag cctgtggttg ccttttgcca ataattcgca cacattgcgg gttactgctt
    19321 tattttcgtt cagtgctgat aacgaacatc aacctgcaaa acgaagcgca tagtttaaaa
    19381 tggctatcat caatacgcta tatctgatgg aaaaaaatat gcctaaactt attttagcct
    19441 ccacctcgcc atggcgtcgc gctcttctgg aaaaactgca aatctctttt gaatgtgcag
    19501 caccagaggt cgacgaaacc ccacgcagcg acgaatcacc gcgacagttg gtgcttcgac
    19561 tggcacaaga aaaagcgcaa tctctggcgt cacgttatcc ggatcattta attattggtt
    19621 cagaccaggt atgtgttctt gatggcgaaa tcaccggtaa accgttaacg gaagaaaatg
    19681 cccgtctgca attacgcaaa gccagcggca atatcgtcac cttctatact ggactggcgt
    19741 tgtttaattc ggcgaatggg catctgcaaa cagaagtgga gccttttgac gtccatttcc
    19801 gtcacctgag cgaggcggag attgataatt acgtgcgtaa agagcatccc ctgcactgcg
    19861 cgggtagctt taagagtgaa ggatttggca ttacgctgtt tgagcgttta gaggggcgtg
    19921 atcctaacac gctggttggt ttaccgctaa tcgcgctgtg tcagatgtta cgtcgggaag
    19981 ggaaaaaccc gctgatggga tagttttttg cgccggatgt ggttacgtcg catccggcaa
    20041 tcggtgctgg cggcctgatg cggcgtaaac gccttatcag gccaaccgcc cattatcagg
    20101 tttatatgct tgtattagcg cgcgttacgc agcttttgca aacaacgctt caaaccttca
    20161 tccatcggag cttcgatacg catcacctca ccggtccccg gatgagtaaa cttcaacgct
    20221 gcagcgtgca ggaacaggcg atttaatccc gtgcctgctt cagtgagctg cctgtcaaat
    20281 tcacggtcac cgtagcgatc gtcaaaggca atcggatgac ccgcatattg tgtatgcaca
    20341 cggatctggt gagtacgccc tgttaccggg ctacaacgca ccagggtggc aaatgcatag
    20401 cgttcttcca ctttaaagcg tgtttccgac ggtttgcctt cctgactcac acgcacgata
    20461 cgttcgccgc tttgcagaat atttttcagt aacggtgctt gaacgctctt cacatgcgac
    20521 tgccactgac cgcgcaccag cgccaggtaa tctttttgca tccctttttc acgtaattgc
    20581 tcatgcagag aacgcaacgc cgagcgtttt ttcgctacca gcaaaacacc tgaggtgtcc
    20641 cggtcaagac gatgaaccag ttcaaggaac cgcgcttccg ggcgcaacgc ccgcaaacct
    20701 tcaataacgc cgaagcttaa accactgccg ccatgtaccg ccgtaccgga aggtttattc
    20761 agcaccagga tgtgatcatc ttcatataag atgacgtccg ccagcgccgc caccttttgc
    20821 agatgtggcg aaaccgcctc ttcttcccgc tcagcaacgc gaaccggtgg aatacgcacc
    20881 tcatcacccg cttcgagttt atattcaggc ttaatacgtt ttttgttcac ccgcacttcg
    20941 cctttacgca aaatacggta aatcatactt tttggtacgc ctttcaattg ggtacgcaaa
    21001 aagttatcga tacgttgccc cgcttcgtca gcggtgatag caacaatttt tacggatgga
    21061 gtctctgttt tcatggttgg cgattctaat tagccaacag gattcgcgcc actcattttt
    21121 ctatgcttat atttactttt gcaccttatt acttcactgc gtgatcactt tattgatggt
    21181 tattaaacca atcaccagca agaagtgaaa aaactgtgag taagcgggtg ataaatggta
    21241 aaagtcatct tgctataaca aggcttgcag tggaataatg aggccgtttc cgtgtccatc
    21301 cttgttaaaa caagaaattt tacggaataa cccattttgc ccgaccgatc atccacgcag
    21361 caatggcgta agacgtattg atctttcagg cagttagcgg gctgcgggtt gcagtcctta
    21421 ccggtagatg gaaatatttc tggagagtaa tacccagtct gtttctttga taattgcgct
    21481 gtttttccgc atgaaaaacg ggcaaccgac actctgcgcc tctttgagct gacgataacc
    21541 gtgaggttgg cgacgcgact agacacgagg ccatcggttc acacccggaa aggcgttact
    21601 ttgcccgcag cttagtcgtc aatgtaagaa taatgagtaa gttacgatga aaagaatgtt
    21661 aatcaacgca actcagcagg aagagttgcg cgttgccctt gtagatgggc agcgtctgta
    21721 tgacctggat atcgaaagtc cagggcacga gcagaaaaag gcaaacatct acaaaggtaa
    21781 aatcacccgc attgaaccga gtctggaagc tgcttttgtt gattacggcg ctgaacgtca
    21841 cggtttcctc ccactaaaag aaattgcccg cgaatatttc cctgctaact acagtgctca
    21901 tggtcgtccc aacattaaag atgtgttgcg tgaaggtcag gaagtcattg ttcagatcga
    21961 taaagaagag cgcggcaaca aaggcgcggc attaaccacc tttatcagtc tggcgggtag
    22021 ctatctggtt ctgatgccga acaacccgcg cgcgggtggc atttctcgcc gtatcgaagg
    22081 cgacgaccgt accgaattaa aagaagcact ggcaagcctt gaactgccgg aaggcatggg
    22141 gcttatcgtg cgcaccgctg gcgtcggcaa atctgctgag gcactgcaat gggatttaag
    22201 cttccgtctg aaacactggg aagccatcaa aaaagccgct gaaagccgcc cggccccgtt
    22261 cctgattcat caggagagca acgtaatcgt tcgcgctttc cgcgattact tacgccagga
    22321 catcggcgaa atccttatcg ataacccgaa agtgctcgaa ctggcacgtc agcatatcgc
    22381 tgcattaggt cgcccggatt tcagcagcaa aatcaaactg tacaccggcg agatcccgct
    22441 gttcagccac taccagatcg agtcgcagat cgagtccgcc ttccagcgtg aagtccgtct
    22501 gccatccggt ggttcgattg ttatcgacag taccgaagcg ttaactgcca tcgacatcaa
    22561 ctccgcacgc gcgacccgcg gcggcgatat cgaagaaacc gcgtttaaca ccaacctcga
    22621 agctgccgat gagattgctc gtcagctgcg cctgcgtgac ctcggcggcc tgattgttat
    22681 cgacttcatc gacatgacgc ctgtacgcca ccagcgtgcg gtagaaaacc gtctgcgtga
    22741 agcggtgcgt caggaccgtg cgcgtattca aatcagccat atttctcgct ttggcctgct
    22801 ggaaatgtcc cgtcagcgcc tgagcccatc actgggtgaa tccagtcatc acgtttgtcc
    22861 gcgttgttct ggtactggca ccgtgcgtga caacgaatcg ctgtcgctct ctattctgcg
    22921 tctgatcgaa gaagaagcgc tgaaagagaa cacccaggaa gttcacgcca ttgttcctgt
    22981 gccaatcgct tcttacctgc tgaatgaaaa acgttctgcg gtgaatgcga ttgaaacgcg
    23041 tcaggacggt gttcgctgcg tgattgtgcc aaacgatcag atggaaaccc cgcactacca
    23101 cgtgttgcgc gtgcgcaaag gggaagaaac gccgacctta agctacatgc tgccgaaact
    23161 gcatgaagaa gcgatggcgc tgccgtctga agaagagttc gctgaacgta agcgtcctga
    23221 acaacctgca ctggcaacct ttgccatgcc ggatgtgccg ccagcgccaa ccccagctga
    23281 acctgccgcg actgtcgtag ccccagcacc gaaagctgca acggcaacac cagcagctcc
    23341 tgcacaacct gggctattaa gccgcttctt cggcgcactg aaagcgctgt tcagcggtgg
    23401 tgaagaagcc aaaccgaccg agcaaccaac accgaaagca gaagcgaaac cggaacgtca
    23461 acaggatcgt cgcaagcctc gtcagaacaa ccgccgtgac cgtaatgagc gccgcgacac
    23521 ccgtagcgaa cgtactgaag gcagcgataa tcgcgaagaa aaccgtcgta atcgtcgcca
    23581 ggcacagcag cagactgccg agacgcgtga gagccgtcag caagctgagg taacggaaaa
    23641 agcacgtacc accgacgagc agcaagcgcc gcgtcgtgaa cgtagccgcc gccgtaacga
    23701 tgataaacgt caggcgcaac aagaagcgaa ggcgctgaat gttgaagagc aatctgttca
    23761 ggaaaccgaa caggaagaac gtgtacgtcc ggttcagccg cgtcgtaaac agcgtcagct
    23821 caatcagaaa gtgcgttacg agcaaagcgt agccgaagaa gcggtagtcg caccggtggt
    23881 tgaagaaact gccgctgccg aaccaattgt tcaggaagct ccagctccac gcacagaact
    23941 ggtgaaagtc ccgctgccag tcgtagcgca aactgcacca gaacagcaag aagagaacaa
    24001 tgctgataac cgtgacaacg gtggcatgcc gcgtcgttct cgccgctcgc ctcgtcacct
    24061 gcgcgtgagt ggtcagcgtc gtcgtcgcta tcgtgacgag cgttatccaa cccagtcgcc
    24121 aatgccgttg accgtagcgt gcgcgtctcc ggaattggcc tctggcaaag tctggatccg
    24181 ctatccaatt gtacgtccac aagatgtaca ggttgaagag cagcgcgaac aggaagaagt
    24241 acatgtgcag ccgatggtga ctgaggtccc tgtcgccgcc gctatcgaac cggttgttag
    24301 cgcgccagtt gttgaagaag tggccggtgt cgtagaagcc cccgttcagg ttgccgaacc
    24361 gcaaccggaa gtggttgaaa cgacgcatcc tgaagtgatt gctgccgcgg taactgaaca
    24421 gccgcaggtg attaccgagt ctgatgttgc cgtagcccag gaagttgcag aacaagcaga
    24481 accggtggtt gaaccgcagg aagagacggc agatattgaa gaagttgtcg aaactgctga
    24541 ggttgtagtt gctgaacctg aagttgttgc tcaacctgcc gcgccagtag tcgctgaagt
    24601 cgcagcagaa gttgaaacgg tagctgcggt cgaacctgag gtcaccgttg agcataacca
    24661 cgctaccgcg ccaatgacgc gcgctccggc accggaatat gttccggagg caccgcgtca
    24721 cagtgactgg cagcgcccta cttttgcctt cgaaggtaaa ggtgccgcag gtggtcatac
    24781 ggcaacacat catgcctctg ccgctcctgc gcgtccgcaa cctgttgagt aataattagc
    24841 tcaaagtaat caagccctgg taactgccag ggctttttta tttcatcttt gaatcaacat
    24901 attaaaattc atcgaatggc atccttgcta accaacaatg caaaataggc aaaaaaatgc
    24961 ccgccccagc gatcccagag caggcagaca aaacataccc agtttcatga tatgttttaa
    25021 agcgaaatta tttgttgagc tggaagagcg acaatccctg catatcggta aatgctttat
    25081 acgatgcctg caatgccgtt tgctgcatga tgtaagatga aatagttgca ttccagtcca
    25141 catcaaccag atcgctcatc tgctgcgttt gccctaaagc gcgatcgcta cctaatgaat
    25201 ccagcgactc cagttcgttc agctgcgtgc ctaattccgc gcgaacagtc agcacattgt
    25261 tcagcgagtt tttcagtccg cggttggttt tatctaacgc cgcagcggcg gtttctttat
    25321 ccgcttcgct atccgcgacc ggcgttttca gggctgcgat ggcactatcc agcatggcaa
    25381 aaagattggt ttcagaagcg ctaccgtctg gttccgctac cgcgttgctg gtaatactgt
    25441 cgaaaatttt gtcacccgtg tgccctatca ccatcgaacg cgaagcatcg acctgttgtt
    25501 taatactttc tgctccaccg acgtatttcc ctttctcttc gctaaacggc gcagtctctg
    25561 ttttataacc ggcaaaaatg tagcgcccgt taccgtcagt ggtgttcgcc agattcagca
    25621 actggtcacg aagcccctga atatccgtag ccagcgaggc ccggtcatcg tcactcaaag
    25681 tgccattgct ggcatagaca attttttcct gagcattctg gatagcagtg gtgacctggc
    25741 taagtacact ctcttccagt gacacttttt gagtggcgaa agttcgcgcc agcgtgtact
    25801 ggctgttttg cgcctgtgcc tgggagagaa ctaccgcttg tgatgctgca atgggatcgt
    25861 cagaagggtt aacgactcgc ttacccgtcg acatctgctc gccgtacttc atccattctg
    25921 cctgagaatt ggtgatgccg cgcatgtttt gctggtacat catctgggta ctgaaacgca
    25981 ttttatctcc ccttagcgaa tgttaatcag cgcatcaaaa atcgcgtttg ccgtctgcag
    26041 aacctgcgca ttcgccaggt aatactgctg aaaacgttgc agatttccgt actcctcatc
    26101 gagattgaca ccggaaatcg actgctgctg attggaaagc tgcgtcacca cattaccttg
    26161 cgtggtgcta ctggttttca acgtcgcggt tttattaccg atatcactca ctaacgaggc
    26221 ataagcgtcg ttaaaggatt tcgctccgcc caccgtttta ctgttgcttt gcagatccag
    26281 cagggcctga ccgttgcggt tgtcgctatc acccgcatct tcttcgctcg ccatcgcgat
    26341 tttcgcttcg tcggtgatta atacatccat gttgacgatg gcgtcactta ctggtttcag
    26401 cgtgaagctg tcgttaacgg caggcgttcc tgtaaacgtc aactccagac catcaaatgc
    26461 cactttaccg ttggcatctg gtgtcaccgt aaaagtggta ttgctggcaa ggcgggtgac
    26521 ctgccactga ttattatcga acgagatttt gtaatctgtc gccagtacca cggaggcatc
    26581 agttaccgtg gcaccgatcg caacgttacc gttgttttta gtgttttgca gaaccgcggg
    26641 cttaccgata gcaaagaaat cttcaccctc atcgccgttg gcatcaaatc cggctttgtg
    26701 ttggctgttg aaagcctcgg caaatgccag cgccagttgt ccaagcgtat tacgcgtctg
    26761 gtccagatcc tgagaacgga atgtcagaat gccgcccagc gacccggtat tcagtaattt
    26821 ctccgggatc tcaatattgc ctgccgtccc atcaacgtaa gcgacagtcg tacgagaagg
    26881 gtcggcgctg gaaggaactg ccgccagttg ccgcgccgta cttccctgaa ccagtgagta
    26941 accattggcc atcgtgatgt tataagtacc gccatcctga acgctgactt ctacgccaac
    27001 aatctggttt aattcgctca ccagttgatc gcgttgatcc agcagattgt taggtgaggc
    27061 ccctgccccc acgcctgtca ggcgcgagat ttgatcgttc aggctggcaa tttgtttagc
    27121 gtaattgttg atctgatcaa cgctggcacc tatcgcgata ttgacctgtt tgtcctggtc
    27181 gcgcagatat tgatcggtgg ttttaaactg attcaccaac ccttctgatt tcccaatcag
    27241 cgcctggcgc gctgccgggt cttccgcgtt actcaccagc gtttgcaggc tggtgaagaa
    27301 atcctgcatc tgtgttgcca gcgaagaggt actggtggag agcatattgt cgattttcga
    27361 catctgctca tagcgggcag tcaggccgct actttgcgtc tgcgccgcac gtaactggtt
    27421 ggtaataaaa gcatcatact cacgctgcac accagaaacg tagacaccat tgccaaccca
    27481 gccgccagcg cccaacgtgc tattggcctg cgccataata gtggtttggc gggtatatcc
    27541 ggcaacgtta tagctggaga tattattact tgccgtattt aacgccgcct gggccgcgtt
    27601 cagtccgctc atggcgttat tgatcaagct ggacatggag gttccttata agccttcaat
    27661 tacagagtat tatcggcagc gacccgccgg acttgagtta ttcagaacag attatcaatg
    27721 ttcatgctgt aggttttgct caccttgtcg cttatcgatt tcatctgctg gatcatgttg
    27781 gtgagtttac gggcatagtg aggatcggta gcataccccg cgtcctgtag ggcctgcgcc
    27841 ccctgctccg cactcgcggc ggtcgtcacg gcggcgtagc gcgggttacg tgttaacagc
    27901 ccaacgtaat ccgacaatgc ttccagatac gagctgtaga cccgaaactt cgcttttact
    27961 ttcttcgctt cgccgttttc atattcagtc gtggtgattt cagtaactgg ccctttccag
    28021 ttgccagagg ctttgacacc aaacaggtta tagctcggct cgccgttttc gcggcggatt
    28081 tgccgttgcc cccaaccaga ttccagtgcc gcctgagcga ggatcaaatg atgtggcaca
    28141 ccgctttgct ggcttgccag ttgggcgggc agcgagagtt gcgcgaggaa tgctttactg
    28201 tcacccggca gcgaatcatc gtagttacgt ggcacggcct tttgcaccag ctgcgaaagc
    28261 gcctgatttt gataacgcac cacagtttcg agcgggaatt tcatcggtgc tgctggcgta
    28321 gactcctctg gcaatggttg ttctggcgtc atctgtttaa ccatcatctc tgcaagcccc
    28381 agacctttgc ccgccgtcat ctgttgggca atctgctggt catacatact ggtatacagg
    28441 cgagtgtgct cgctgctgaa caggccatct tttggtaaag cgtcgcgcat gcttttcaac
    28501 atcatctgca cgaacatccc ttccacctga cgggccaccg gacggatatt tgccgccgga
    28561 tcttcgcccg ctttcgcctt tagttcgttg agtgattgcg catcccaggc cgcacttgcc
    28621 agtagtttgc tgtcgctgat catcagatga tttccagttt tgcccgcaga catcccgcac
    28681 tttgcattga ttgcagtatg gacatcagat ccatcggcgt agcgcccagc gcattgagcg
    28741 cgcgcaccac gttattgagg ctggcgctgg aacgtacgct ttgcagcgaa ccgccgctct
    28801 ggcgtaaatc gatctgcgtt tgtggagtaa ccacagtctg tccaccacca aacggtgtat
    28861 ctggctggct gacattggcc tgacgattaa ctgttactga gagattcccc tgcgctaccg
    28921 cgcagctgtc gagggtcact tcgcgattca tcaccaccga accggtgcgc gagttaatca
    28981 ctactttagc gtcctgcggg gtgacattaa catgcatatt ctgaatatcg gcaaggaagc
    29041 ggacctggga actgttgcca ctcggtacgc gcacctgaat agtccgcgca tctaacgcgg
    29101 tggcgctgcc atatccacgc acacggttga tggtatcagc gatttgctgc gccatgctga
    29161 aatcttcgtc gttaagttgc aaattaaggg tattcccgac gccaaactgg ctgggcaatt
    29221 cacgttcaat aaccgcacca ttggtgatcc gtccaccgtt cagttggtta acctgaacac
    29281 tgctaccgcc agcggaggct cctgcgccgc caaccagaat attgccctgc gccagcgcat
    29341 acacctgact gtcaacgccc ttaagcggtg tcatcaacaa cgtacctcca cgcaagcttt
    29401 tggcattacc catggaagaa accaccacat cgatggtttg cccctgacgt ccaaacggag
    29461 gaagtgacgc tgtcaccatt accgcagcga cgttttttag ctgcatattg gtgcccgtcg
    29521 gaacggtaat tcccagctgt gagagcatgt tattaagcgt ttgtgtggta aacggcgtct
    29581 gggttgtctg gtcaccggtg ccatccagcc ccaccaccag accatagcca atcagtgagt
    29641 tttgccttac cccctgaaca ctggtgagat cgcgaatacg ctcagcctga gccgccgtcg
    29701 tgaccagtag aagaattaat gcagagagaa atttaatcac cacagcctca cttacattgg
    29761 cgacaggtta aggaagaaac gctgcaacca gcccatattt tgcgcttcat taatgtagcc
    29821 attgcctacg tattcaatgc gcgcatccgc cacctgagta gacggtacgg tattgctgcc
    29881 gctgatagtg cgtggattaa ccacgccaga gaagcgaata aattcggtac cctgattaat
    29941 ggcaatctgt ttttcaccca ccacatgcag gttgccgttg accagtacct ggtcaaccgt
    30001 caccgtcaac gtgccgctaa aggtattgct ggcattggcc ccgccctttc cgttgaacgt
    30061 gttaccaccg gaggcttcga catcggcacg agcgttacca aacagcccct gcaaatagcg
    30121 cggcacagta tcaaagccaa aattagtttt accgtcacgg ctggcattcg cagaggagct
    30181 tttgctggcg ctgacgttct cctgcaacac gatggtcagc gtatcgccaa tattgcgtgg
    30241 tcgacgatct tcaaacagcg gttgatagcc atagttaatc ggctgagcag actggaaaat
    30301 agaaccgttg gcgacgggcg tcggaccggg aaccggttgt gcactggtcg ccccctgcac
    30361 cagcggcgtg gagggtatcc aggcgcagcc ggttagtgaa agcaccaaca agctggaaat
    30421 ggcataagta tgcgcagcgt ttttttgcat ggctatcatc ttcaaaaatc agtaaaccgg
    30481 tgaacctgcc accggttaag ccttagagtt gcgtcagttt ttgcagcatc tgatcggtgg
    30541 tggacaccgc tttactgttg atttcgtaag cgcgttgcac ctgaatcata ttgaccagtt
    30601 cttccgccac gttgacgtta gacgtttcaa catacccttg atacagcagt cccgcgccgt
    30661 tcaggcccgg cgtgctttcg ttcggtgcac cggaggattg cgtttcggtg tagaggtttt
    30721 cgccaatgct ctccagcccg gtatcattca taaaggtggt gagattgagc tgcccaacct
    30781 gaaccggagc tgcctggcct tgttgggtta cgctgaccac gccatcacga ccgatggtga
    30841 tacttaacgc attcgccgga atggtgatcg ctggctgtac ctgaaaacca ccagccgtca
    30901 ccagctgccc gttctgatcc acctggaaag agccgtcacg ggtataggct gatgagccat
    30961 ctggcaacat cacctggaaa aagccctgcc ctttaatcgc gacatctttg ctgttgttgg
    31021 tctgcgacag gtttccctgg ctgtgtaagc gttcagtggc gaccgggcgt acccccgtgc
    31081 cgatttgtaa tccggagggt aaggtggttt gttcggaaga ctgtgccccc ggctggcgaa
    31141 tggtttgata aagcagatct tcaaacaccg cgcgctgacg cttataaccg ttagtactga
    31201 cgtttgccag gttgttggca atgacgtcca tattggtttg ctgggcgtca aggcccgttt
    31261 tggcgatcca taatgaactg atcataaaat gtcctgtagt ggattagtga caaagtgcat
    31321 atgtcttctt gccggatgcg gcgtaaacgc cttatccggc ctacaagtgc tcgcaaaatc
    31381 gataaattgc gattgccccc atagacccga taaacttgcg catcgggcaa cttatacttg
    31441 tcatgtatcc tttcaattaa ctcatcgaca gcagttggtt ggcacggcct gcgttatcat
    31501 cgacgctgct gatcaccttc atctgcattt caaaacgccg cgcgctggca atcatgtcgc
    31561 tcattgccgc aacggcattg acgttactgc cttccagaac ccccgacatc acacgcaagg
    31621 ttggatctgc ctgcagtatc ggcccacgcg tggcctggct ttctgcgctt aaacgaaaaa
    31681 tgccgtcgtc accgcgttgc acttcgctgc ccgtggcttt caccagtttc agacgcccca
    31741 ctggcgcaac cgtatttgcc ggatcgcccg gattgagcgc cgagattgtg ccatcggcag
    31801 caatagtgat ttccgcccct tccggcacag caattggccc cgcctcgcct atcaccggat
    31861 gcccctgaat tgtcagttgc ccggtgggat caacctgaat gctgccatta cgcgtatacc
    31921 cttcgctgcc gtcagcggtc tgcacggcca gccagccatc ctgctgcaac gcaacgtcca
    31981 gcgggcgcga ggtgtaatcc atcttgccgg gcgtcatgtc tgcacccggt gttgacgccg
    32041 ttaccagcgt gcgcgtaggc agtgaaagcc cttccaccgg cactgcacgc aaggcgttta
    32101 actgcgcgcg aaaaccgggc gttgaggcat tggccagatt actggcggtt accgcctgtt
    32161 gattcagcgt ctggctggct gctcccatcg cggtataaat tgcgtgatcc attgagctat
    32221 cccgtcagcg attagcgtaa gttaaccagc gtgttgagga tctggtcctg ggttttgatg
    32281 gtctgggcgt tagactgata gttacgctgg gcaacgatca tattgaccag ttctttactg
    32341 agatcgacgt tggacgcttc cagcgcaccg ttggtcaggg tgccaaagtt gcccgtcccg
    32401 gctgtcccca acagcgccac gccagaagat tgcgtcgcag accagacgtt gtcgccttcg
    32461 gatgccagac cttcgttgtt ggcaaagttc gccagtacaa tctgccccag cagttgggtt
    32521 tgttcgttgg aatagttgcc gacaaccgta ccgtcatcat tgatttgata actcaccaga
    32581 tcgcccggtt tgtagccatt ctgggtggtt gccacaatgt tgttagcgcc ggtattttgc
    32641 tgcatggagt tgaggaagct caggctaaac gtggcgggtt ctgcgccgtt aattgcgccg
    32701 gttgcgatat tattcgccat cgcaccatcc actaatgtgc cattagcatt aaatttcagt
    32761 gttgtcgctg tcttcgcaat gctgtttgga tcactgctat cctgggtgta gacctgccag
    32821 ttattatccc cggtcttcac aaagtagacg ctcatgtcat gagcattacc ctgactgtcg
    32881 aaaacagtca ccgaaccttt tttgttatag ctatccgcat tgctggcatc aaatgcgtta
    32941 acagagggaa gcggatcgct ggaattcagg ttgatctgca tcgacgccgt agtggtagtt
    33001 ttcgctgcca tcagggtatt cgggatcgaa atattagtcg gattcgcccc ttgctgaata
    33061 gtcggcggcg taccggttgc cgggtaaccc gtcagctgta aaccttgcat attcaccagg
    33121 ttacggtttt catccagctt aaattgtccg ttacggctgt agaacaccga accgttgcta
    33181 tctaccagac ggaaaaaacc gttctggctg atagcaacgt ccagaccgcg cccggtgttg
    33241 gtggtcgtgc catcggtaaa gtcctgagtg ataccggcaa cttttacccc cagtcccact
    33301 ttcgaaccgg caaacatatc ggcaaaagag gccgtgcctg atttaaagcc gtaggtggcg
    33361 gagttggcga tattgttgcc aataacatcg aggttggtgg cggcagcgtt taatccgcta
    33421 accgcttgag aaaaggccat gactgactcc tgaaatgtga aggcttaaat tatctgccgt
    33481 acttcgtcga gggtggtggt gccgtaagtg ccgagatcca gcgtattacc gttgttgccg
    33541 cggatcacac cctgcaccag agcaaactgc agcggctggg caaccaattg tgtaccaccg
    33601 ttactggcgc taatcgctac gttgtaagaa ccgttcggcg cagtgctgcc gtcagtcaac
    33661 gtaccgtccc aagtgaaact gtggactccg gcggtcagtt caccaatatc aatggtgcgc
    33721 acaaccgcgc catttttatc ggtgatggtg gccgtaactt tgtctgccgc ctgttgcagc
    33781 tcaacaccaa acggcgtggt cgtggtcaca gccccttctt cactaccggt tcccgcaaga
    33841 acagtggtgc cggggatcat cacgccgtga ccgatcaggt tactggcctg taacgactgg
    33901 ctgttatcaa tctgtccgga aatagagccg agcgtggtat tgagtttttc aatcccgctg
    33961 accgtgctga tttgtgccaa ttgcgacgtc agctcgttgt tttccattgg attggtcggg
    34021 tcctggtttt tcagctgcgc caccagcaaa gtcagaaaac tgctttgtaa atctgcggcg
    34081 ttgctgcccg tgagcgaact actgctggtg gtactgacgc cggtatttgt cggatcggtg
    34141 gtggttaccg caatggacat agctttctcc tttattgacc gagcgtaagg gttttcagca
    34201 tcatgctttt caccgtgttg agcacttcaa cattggcctg atagctgcgt gacgccgaca
    34261 tggtgttaac catctccccg acgacatcaa cgttcggcat ttttacgtag ccctttgcat
    34321 ctgccagcgg attacccggt tcataaacca gtttgtccgg ggcctgactt tctataacat
    34381 cggcaacctt tacgccgcct gtcgcagcac ctggtgcagc gttaacctgg aataccacct
    34441 gttttgcccg atatggctgt ccatcaggac cggtcacgct atcagcattt gccagattac
    34501 tggccgccac gttcaggcgc tgggactggg cagttaacgc cgacccggcg atatcaaaaa
    34561 tattcagcag tgccatccgt taatttccgc tctgtaaaac gttcatcatg cctttgattt
    34621 gcccgctcaa cgcgctaagg ctcatctggt attgcaggct gttatcggca aactgggtgc
    34681 gttcgcgatc catatcgacg gtattaccgt caagcgaagg ctggtccgga atacggtatt
    34741 gcagttctgc ggtaggaggc gtcagcgcct gcgccgggat gtgttgcgtt gaggtcatcg
    34801 tcagtgcaac cacactggtt gcatcccgtc cacgttgcat gactttttta agttcactgg
    34861 caaaatcgat atcgcgcgcc tgataaccag gggtatcggc attggcgatg tttgctgcca
    34921 gcacttcctg acgctgggcg cgcagattga gcgcctcttg ttgaaaacgt aaggcggcgt
    34981 cgagcttatc gagcatatct cctccgcagg tatcaaaatt ctgccatcag cttaaatgcc
    35041 tttacgcgcg cgttatcggc ggaataaacg caaaatgggt cgctatttat gccgttgatg
    35101 accattgcgg acaggtacaa ttcacgttgt agaaatggct ggggggcgaa aatgctggca
    35161 ataaaacgta gcgtggcgat cattgcgata ctgttcagtc cgttaagtgc ggcgagcaat
    35221 ctcacatcgc aattgcaaac cttttttagc gcccagctcg cgggggtaag tgatgaggtt
    35281 cgtgtttcta ttcgtacagc gcccaatcta ctaccgccgt gcgagcagcc attgctttcg
    35341 atgagcaata attcccgcct gtggggcaat gtgaatgtgt tggcacgctg cggtaacgac
    35401 aaacgatatt tacaggttaa tgtacaggcc acaggaaatt atgtggttgc cgcgatgccc
    35461 attgcgcggg gaggaaagct ggaagctggc aatgtcaaac tgaaacgcgg acggctggat
    35521 accctgccac cgcgtacggt gctggatatc aatcaacttg ttgatgccat tagcctgcgc
    35581 gatctatcac ccgatcaacc tatccagtta acccagtttc gccaggcatg gcgggtaaaa
    35641 gcgggacaac gcgtcaacgt gatcgccagc ggtgatgggt ttagcgccaa cgcagaaggt
    35701 caggcgctga acaatgcagc cgtcgcacag aatgcgcggg tgcgcatggt atcgggacag
    35761 gtagtcagcg gcgttgttga tgcagatggg aatattctta taaacctgta agctgttaaa
    35821 gattacccgt cccttgccga taaataagca acacatgata aaagcgccct caatgaggaa
    35881 taaaccatga gtattgatcg cacttcgcct ctgaagcctg taagcaccgt tcaaccgcgc
    35941 gaaaccactg acgcgccggt aacgaacagc cgggcggcaa aaacaaccgc ctccaccagc
    36001 accagtgtga cgttaagcga cgcgcaagca aaactgatgc aacccggcag cagtgatatc
    36061 aatcttgaac gtgtcgaagc gttaaaactg gcgattcgta acggtgaact aaaaatggac
    36121 accggcaaaa ttgccgatgc gctgatcaac gaagctcagc aagatttgca gagtaactga
    36181 tcgtatgaca cgtcttgcag agatcctcga ccagatgtcc gctgtgctta acgatctcaa
    36241 aacggtaatg gatcaagagc agcaacatct ctctatgggg cagatcaacg gcagccagtt
    36301 gcaatggatt acagaacaaa aaagctcact gctggcgacg ctggattacc tcgaacagtt
    36361 acgcaagaaa gaacccaata cagcaaatag cgttgatatt agtcaacgct ggcaggaaat
    36421 tactgtgaaa acgcagcaac tacgccaaat gaatcaacat aacggctggt tactggaagg
    36481 acagattgag cgcaatcaac aggcgctgga aatgttgaaa ccgcatcagg aaccgacgct
    36541 atatggggcg aacggtcaga cctcaacaac ccatcgcggc ggtaaaaaga tttcgatctg
    36601 aatattatct ccggcctgca ctgcaggccg gaatgcattg ttacaccgtc cggcgggcaa
    36661 attctttaac tttgaagccc agtaccgcca gtgcagcgaa gtacgcggca atccccgcca
    36721 gcacgaccgc cattaaacgc agtaagcgcc agggcatggt acccaatgac cactccggca
    36781 tgatatgtaa catacctaaa agcacgccag acatcaccag taccgccacc accagacgca
    36841 acagaaacgc catccagccg ggttgcggag taaagatttt ctgcttacgc aactgccagt
    36901 aaagcagcga agcattcaga cacgccgcca gaccaataga aagtgacagc ccggcatgtt
    36961 tcaacggacc aataaacgcc aggttcatca attgcgttaa aatcagcgta acgatggcaa
    37021 ttttcactgg cgttttaatg tcctggcggg aataaaagcc aggagccaac actttcacta
    37081 caatcaggcc aatcaaaccc accgagtagg caattaacgc ccgctgggtc atcagcgcat
    37141 caaacgcggt aaatttaccg tactggaaca gcgaaacggt taacggacct gaaagaatgc
    37201 ccaacgcaac cgcactcggc agcgccaaca ggaaacaaag acgcaacccc cagtccatca
    37261 aacggttgta ttcatcatga ttgccactgg caaaactttt cgacagcgat ggcagcaaaa
    37321 tggtgccaag cgccacgccc agcacaccgg agggaaactc cattaaacgg tcggcgtaat
    37381 acatccaaga caccgaaccg gaagcaagaa acgaggcaaa aatggtgttg atgattaagg
    37441 agatctggct cacagagacg ccaaggatcg ccggtcccat ctgtttcacc acgcgcattg
    37501 ctccggcatc gtggaagtta atgcgcggca ggaccagcat gccgatcttc ttcaggtgcg
    37561 gtagctgata caccaactgc aggatgccgc cgaccgtaac agcccacgcc agcgccagca
    37621 ccggtgggtt aaagtacggt gcggcaaaca gcgcgaaacc aatcatgctg atgttaagca
    37681 gtgttggcgc aaacgccgga atcgagaagc ggttccacgt attcagaatc gctcccacca
    37741 gcgacgccag ggagataagc aagatatagg gaaaggtaat ctttagtagc tggctggtca
    37801 gggcaaattt gtcagctgtg tcagcgaagc ctggcgcggt caccatgatc acccacggtg
    37861 cggcgagcat gccagcgacc gtcacaaccg ccagcgcaag tgtcagcagg ccagaaacat
    37921 aagagacaaa gacccgcgtg gcgtcttcac cctgcttact tttatattcc gccagaatcg
    37981 gtacaaatgc ctgggaaaat gccccttcgg caaagatacg gcgtaacaag ttaggaagtt
    38041 taaaagcgac gaaaaaggcg tcggttgcca tccctgcgcc aaagattctg gcgacaattg
    38101 cgtctcgtgc gaagccaagc acacgcgaaa acatggtcat cgagctgacg gcggccagcg
    38161 attttaataa attcatcggt gttctaatcc agacccacgg gcaaaacgcc tgcaaggcag
    38221 gcgctgaaag aggcgaatag tctacctgga ttatggtgaa ttgctaccgc cagatgttac
    38281 agggtttatt cactcatcgc atcgcgccag atcttgtcaa cgatacgttg cgcccgcact
    38341 gcctgttcgc cggcggtttg cggaactgtc tgattttgca cacattcaat gaagtgccgc
    38401 gcacagccga caaatccacg ttgctcaaga gtgctctgcc aaccaggaat cggtttattc
    38461 actacgccct gcccacgctc ctcacgccat tcgcgcatat ccgtaatgtc gatgagcgca
    38521 ccgtcagtca cggcctgcac ggtttcacgc tggcttccgg cacggcgatg catacaggtg
    38581 gtgatctgca aaggaccggc cgaaaaatgg tgctcggcaa acagcatttc gccagcgtcg
    38641 ttagtcagta gcgtaccgcc atccatagag gctttgccgc ccgacaacca cagcgcggta
    38701 tccaccacat gcagataatc atccagcaac gtgaaataaa gatcgtgtgg cccgacgcta
    38761 ttgctacgat gtttatccat tcttagcgag gctgcggtgg cgagttgcgt ttttaactca
    38821 ccgtagagtg gtgcgaaacg acggttaaaa ccgaccatca acgtcaattt tttccgcgcc
    38881 gccagttcca ccagccgttc agcatcgcgc agattttctg ccagcggttt atcgacacag
    38941 acatgtaccc ccgcattgag taacgtacta accacgtcaa agtggctggc ggtgctggaa
    39001 tgcacaaaaa ccgcatcgca actggcggca aggctggata acgaatcggc ataaggaatg
    39061 cgccagcttt cacaaattgg cagggctttc gcgcgcgtag gcgaccaggc tccttgtaac
    39121 atccagtcag acgctgccgc cagcaccggt aaccacgctt tttgcgcaat gccgcctaat
    39181 cccactacgc cgatacgtaa ttttttcact ttaatctccc agatgggcca gcaacgaatc
    39241 aagacgctgt ttcagttctg ccacttcgat ttccagagct tcgacgcggg cctgtaaatc
    39301 accgtcaacc gcgttcgaca tatccgttac cgccggctga tcttcaacct caccactgaa
    39361 aagatgcatg tagcggcttt cacgtttacc cggttcgcgg gccagacgca ccacaaaagg
    39421 accatcttcg cgatttgcca gttgttccag cgtcgactca acttccgcca tatcgctgaa
    39481 ttcatacatt cgcgcggcgc ggctgcgcag ttcacccggc gtctgggcac cacgcaataa
    39541 caacgtggtg attaacgcca cttctgcggc gctcagtttc agatcgccaa attctgaatt
    39601 acaaaaacgt tgttcatatt tggtgacccg attaccaaag ccactcaccg tgcgcagata
    39661 atggcgtttg accagattat ccagttgctc ctgcacctcg gattcactca gattcatgac
    39721 cggttcacgg ttcgttttct gattacaggc cgtgactaca ccattgactg agagcgggta
    39781 ttgctccggc gtggtcacct gcttttccag caagcaacca atcacacgag cttccagggc
    39841 agtaagttga tatttcatga gattctcctt agcggccggg cgtccagtct ggggtagtta
    39901 atgccgtcag tacgtgatcg cgccattgtc catcaatcaa cagatagtct ttcgcatagc
    39961 cttctttttc aaaacccagt cgtgccagta aatcaccgct gcgtttattg tgcggcatat
    40021 aattagccat aatgcgatga atatgttggg tgcgctgcat ataacgaatg gctgcggtca
    40081 gggcttcaaa catgagtcct ttgccctgcc atttttgccc aatcgaataa ccgagatagc
    40141 aggcatgaaa agagccgcga acaacattgg aaaaattggc aacgccaata atctcttttt
    40201 cgtccgggtc gaataagcca aagtagaacg ctgaaccttg tttatgaaat tcgttaatca
    40261 tccccagcct ggcctgccag cctgatggat aacagtggct ttcgtcgcgc actggctccc
    40321 agggcttgag gaaatggcga ttctctgcgt aataatccgc aagacgccag gcatcacgat
    40381 catgcaccag acgcacgacc agtcggtctg tggttaagcg cacttttggc acgttactgc
    40441 gatagccaaa cattctatac ctactccttc ccgtaacgtc tttaatacgc tgtaagtatt
    40501 actatacctg cgcaaccaga ccctgtgaaa agtccaaaat gccattgtct gcattattca
    40561 catatttgat aaaaactctc tatcaaagcg acgttttgat aaaaaaatat tgtccccgaa
    40621 gtgatgggaa aagtcttagc ggcaatgcag aatagaaagt gctcatcttt tatttcccgg
    40681 gaggggaaat gtcccgcgta tcgcaggcga ggaacctggg taaatatttc ctgctcatcg
    40741 ataatatgct ggtcgtgctg gggttctttg ttgtcttccc gctgatctct atccgcttcg
    40801 ttgatcaaat gggctgggcc gccgtcatgg tcggtattgc tctcggtcta cgccagttta
    40861 ttcagcaagg tctgggtatt ttcggcggcg caattgccga ccgctttggt gccaaaccga
    40921 tgattgttac cggtatgctg atgcgcgccg ccggattcgc cacaatgggt atcgcccacg
    40981 aaccgtggct attgtggttt tcatgcctgc tctcgggact cggcggcacg ttgtttgatc
    41041 cgccgcgttc ggcgctggtg gtgaaactaa tccgtccaca acaacgtggt cgttttttct
    41101 cgctgttgat gatgcaggac agtgccggtg cggtcattgg cgcattgttg gggagctggc
    41161 tgttgcaata cgactttcgc ctggtctgcg ccacaggggc cgttttgttt gtgctgtgtg
    41221 cggcgttcaa tgcgtggttg ttaccggcat ggaaactctc caccgtacgc acgcccgttc
    41281 gcgaaggcat gacccgcgtg atgcgcgaca agcgttttgt cacctatgtt ctgacgctgg
    41341 cgggttacta catgctggct gtacaagtga tgctgatgct gccaattatg gtcaacgacg
    41401 tggctggcgc gccctctgcc gttaaatgga tgtatgccat tgaagcgtgt ctgtcgttaa
    41461 cgttgctcta ccctatcgcc cgctggagtg aaaagcattt tcgtctggaa caccggttga
    41521 tggctgggct gttgataatg tcattaagca tgatgccggt gggcatggtc agcggcctgc
    41581 aacaactttt caacctgatt tgtctgtttt atatcgggtc gatcattgcc gagcctgcgc
    41641 gtgaaacctt aagtgcttcg ctggcggacg caagagctcg cggcagctat atggggttta
    41701 gccgtctggg tctggcgatt ggtggcgcta ttggttatat cggtggcggc tggctgtttg
    41761 acctgggcaa atcggcgcac cagccagagc ttccgtggat gatgctggga attattggca
    41821 tcttcacttt ccttgcgctg ggttggcagt ttagtcaaaa acgcgccgcg cgtcgtttgc
    41881 tggaacgcga cgcctgattt gatactcccc cctgactcct ccatactgaa agtgaactga
    41941 cagccaaatg gaggagtcac gtgaagctat acatttacga tcactgccct tactgcctca
    42001 aagcccgcat gattttcggc ctgaaaaata tccccgtcga attacatgtt ctgctcaacg
    42061 acgacgcaga aacacccacc cggatggtcg gtcaaaaaca ggttcccatt ctgcaaaaag
    42121 atgacagccg ctatatgcca gaaagcatgg atatcgttca ctatgtcgat aaactcgacg
    42181 gcaaaccgtt actgaccggc aaacgttccc ctgccattga agagtggctg cgcaaagtca
    42241 atggctacgc caacaaactg ctgttgccgc gttttgccaa atcggcattt gatgagtttt
    42301 ctactcccgc cgcgcgcaaa tatttcgtcg acaagaaaga ggccagcgcg ggtaattttg
    42361 ccgacctgct ggcccactct gacggtctga ttaagaatat cagcgatgat ttgcgtgcgc
    42421 tggacaaact gatcgtcaaa ccgaacgccg tgaatggcga actttcggaa gatgatattc
    42481 agctatttcc gctactgcgt aatctgacgc tggtagccgg aatcaactgg ccaagccgcg
    42541 ttgctgatta ccgcgataat atggcgaaac agacacaaat caatttgtta tcatcaatgg
    42601 cgatttaatc ctgacccgcc gcggcagcct gtattcgcgg gttgccgccg ttttcagttt
    42661 tttctgctgg cgagtttatc gctggtgaca catgctgcgt tgtgtcagga tattgacgca
    42721 gatttcatgt cgaggactac catgaacaag tttttatttg ccgctgcatt gatcgtcagt
    42781 ggcctgctcg ttggctgtaa tcaactcacc caatacacca tcaccgaaca agaaattaac
    42841 cagtcgcttg cgaaacataa taatttctca aaagatatcg gtttacccgg cgtggctgac
    42901 gcccatattg ttctgacaaa cttgaccagc caaattggtc gcgaagagcc gaataaggtt
    42961 accctaaccg gagacgccaa tctggacatg aactccctgt tcggtagtca gaaagcgacc
    43021 atgaaactga agctgaaagc gctgcccgtg tttgataaag aaaaaggtgc gatcttcctg
    43081 aaagagatgg aagtggtcga tgcgacggta caaccggaaa aaatgcaaac ggtgatgcaa
    43141 acgttgcttc cctatttgaa ccaggcatta cgcaattact ttaaccagca acctgcttac
    43201 gtcctgcgcg aagatggcag tcagggcgaa gcaatggcga aaaaactggc gaaaggcatt
    43261 gaagtgaagc caggcgaaat tgtcattccg tttacggatt aatgacgagg gcgcattcgc
    43321 gccctttatt tttcgtgcaa aggaaaacgt ttccgcttat cctttgtgtc cggcaaaaac
    43381 atcccttcag ccggagcata gagtttaatg actgcaccat cccaggtatt aaagatccgc
    43441 cgcccagacg actggcacct tcacctccgc gatggcgaca tgttaaaaac tgtcgtgccg
    43501 tataccagcg aaatttatgg acgggctatc gtaatgccca atctggctcc gcccgtgacc
    43561 accgttgagg ctgccgtggc gtatcgccag cgtattcttg atgccgtacc tgccgggcac
    43621 gatttcaccc cattgatgac ctgttattta acagattcgc tggatcctaa tgagctggag
    43681 cgcggattta acgaaggcgt gttcaccgct gcaaaacttt acccggcaaa cgcaaccact
    43741 aactccagcc acggcgttac gtcagttgac gcaatcatgc cggtacttga gcgcatggaa
    43801 aaaatcggta tgccgctact ggtgcatggt gaagtgacac atgcagatat cgacattttt
    43861 gatcgtgaag cgcgctttat agaaagcgtg atggaaccgc tgcgccagcg cctgactgca
    43921 ctgaaagtcg tttttgaaca catcaccacc aaagacgcag ccgactatgt ccgtgacgga
    43981 aatgaacggc tggctgccac catcactccg cagcatctga tgtttaaccg caaccatatg
    44041 ctggttggtg gcgtgcgtcc gcacctgtat tgtttaccca tcctcaaacg caatattcac
    44101 caacaggcat tgcgtgaact ggtcgccagc ggttttaatc gagtattcct cggtacggat
    44161 tctgcgccac atgcacgtca tcgcaaagag agcagttgcg gctgcgcggg ctgcttcaac
    44221 gccccaaccg cgctgggcag ttacgctacc gtctttgaag agatgaatgc tctgcagcac
    44281 tttgaagcat tctgttctgt aaacggcccg cagttctatg gcttgccggt caacgacaca
    44341 ttcatcgaac tggtacgtga agagcaacag gttgctgaaa gcatcgcact gactgatgac
    44401 accctggtgc cattcctcgc cggggaaacg gtacgctggt ccgttaaaca ataaaaatcg
    44461 tagcttcctg ttgtcattag gttattttac ctgtataaat aaccagtata ttcaacaggg
    44521 ggctattatg cgaattgaag tcaccatagc gaaaacttct ccattgccag ctggggctat
    44581 tgacgccctg gctggcgaac tttcccgccg tattcagtat gcgtttcctg ataatgaagg
    44641 ccacgtatcg gtacgttatg ccgcagcgaa taatttatcg gttattggcg caacaaaaga
    44701 agataaacag cgcattagcg aaattctcca ggaaacgtgg gaaagcgccg atgactggtt
    44761 tgtcagcgaa taatatgcag tgatttttgt ttttgccggg tcgccccggc ttttttattt
    44821 tacttacgaa ttttgagtac aaaatttcta cacttcctca aaataattgc acaatatgct
    44881 aaaaaaatat gaatgacatg ctgttttatt gttcatataa atcttaaaag cagaaattta
    44941 tctgttcccc cctttaaaaa ttcggtgaat acccttactt attggtatac tgaagttgct
    45001 tcagataaaa acgcattgaa cctcgaataa cgttgtctag taacacgaat tagggggcca
    45061 tgatggaaaa aaataatgaa gtcattcaga ctcatccgct cgtagggtgg gacatcagca
    45121 ccgttgatag ctatgatgcg ctgatgttgc gtttgcacta ccagacccca aataagtccg
    45181 agcaggaagg gactgaagtt ggtcagacgc tctggttaac cactgatgtt gccagacaat
    45241 ttatttcgat attagaagca ggaatcgcca aaattgaatc cggtgatttc ccggtaaacg
    45301 agtatcggcg tcattaacat gacatgacca tccacccaaa aggcacctca ccggtgcctt
    45361 taccatttct gggttgtata tctgcattca gttaattact ctgcttaaac catgagagca
    45421 gagagaaaaa aatgaaatac gatctcatca ttattggcag cggttccgta ggcgctgccg
    45481 ccgggtatta tgcaacccgc gccggtttaa acgtgctaat gaccgacgcc catatgccac
    45541 cgcatcaaca cggcagccac cacggcgata cgcgattaat tcgccatgct tatggtgaag
    45601 gcgaaaagta tgtcccactg gtactccgcg cgcaaacact gtgggatgag ctctcccgcc
    45661 acaacgaaga tgatcccatt tttgtacgct ctggtgtcat taacctcggc ccggctgact
    45721 ccgcatttct cgccaacgtc gcccacagcg ccgaacagtg gcaactcaac gtcgaaaagc
    45781 tcgacgcgca agggattatg gcccgctggc cagaaatacg cgtcccggac aactacatcg
    45841 gcttatttga gactgattcc ggttttttgc gcagcgaact ggcgattaaa acctggatcc
    45901 aactggcgaa ggaagcgggc tgtgcgcagc tgttcaactg cccggtcacc gcaattcgtc
    45961 atgacgatga tggcgtaact attgaaacgg ctgacggaga gtatcaggcg aaaaaagcga
    46021 ttgtctgcgc gggaacatgg gtaaaagacc tgctcccgga gctgcctgtc cagcccgtac
    46081 gcaaagtatt tgcctggtat caggccgatg gtcgctatag cgtgaagaat aaattcccgg
    46141 cgtttaccgg tgaactgccc aatggcgatc aatattatgg ttttccggca gaaaacgacg
    46201 cgttgaagat tggcaaacat aacggaggcc aggttatcca ttcagcggat gaacgtgttc
    46261 cgtttgcgga agtggtcagc gatggttcgg aagccttccc gttcttgcgc aatgtcttgc
    46321 cgggtatcgg ttgctgcctg tacggcgctg cctgcaccta tgataattcg cctgacgaag
    46381 attttattat cgatacccta cccggtcacg ataatacact gctcattacc ggcctgagcg
    46441 ggcacggttt taaatttgcg tctgttttag gggaaatagc tgccgatttt gcgcaagaca
    46501 aaaaaagcga ttttgattta acgccattca ggctttcccg cttccaataa tcatcattac
    46561 ggcctccaga atgaggccgt ttttacagga gtatttaatg cgtcggctgt tgcactatct
    46621 catcaataat attcgcgagc atctgatgct ttatcttttt ctctggggat tgttggcaat
    46681 tatggatctg atctatgtgt tttattttta gttttccgca ctgacataaa ctgacaaata
    46741 tttaatctcc ctttcattta gcgtgaaaaa ttgaatgaga ttattcctgc ttctacagca
    46801 ctc
//
LOCUS       DAPHBJ010000011        46437 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.011, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000011 DAPHBJ010000000
VERSION     DAPHBJ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.011"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..607)
                     /locus_tag="RHZ76_000606"
     CDS             complement(<1..607)
                     /locus_tag="RHZ76_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2811299.1"
                     /translation="MAKISDLNCYQHMASADNVKPKNEVLNTWCVGMNDFARNAEGQD
                     SRRNILSPGAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMNF
                     VMNNPEGERADSSKVIIEISLSTTTTMGTRQGHTAIIFPQSDFLSFRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKNMALLLNNHQLNWKDAIDYNEL"
     gene            complement(730..1182)
                     /gene="tabA"
                     /locus_tag="RHZ76_000607"
     CDS             complement(730..1182)
                     /gene="tabA"
                     /locus_tag="RHZ76_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HDV2811300.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYKARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPETDWLVDKDIAF
                     LPEVVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(1327..1920)
                     /locus_tag="RHZ76_000608"
     CDS             complement(1327..1920)
                     /locus_tag="RHZ76_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDV2811301.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGEAIKTLLQE"
     gene            1991..2704
                     /gene="bdcA"
                     /locus_tag="RHZ76_000609"
     CDS             1991..2704
                     /gene="bdcA"
                     /locus_tag="RHZ76_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV2811302.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAHETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            2835..3230
                     /locus_tag="RHZ76_000610"
     CDS             2835..3230
                     /locus_tag="RHZ76_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDV2811303.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMMVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            3511..3645
                     /gene="pyrL"
                     /locus_tag="RHZ76_000611"
     CDS             3511..3645
                     /gene="pyrL"
                     /locus_tag="RHZ76_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HDV2811304.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            3649..4584
                     /gene="pyrB"
                     /locus_tag="RHZ76_000612"
     CDS             3649..4584
                     /gene="pyrB"
                     /locus_tag="RHZ76_000612"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDV2811305.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            4597..5058
                     /gene="pyrI"
                     /locus_tag="RHZ76_000613"
     CDS             4597..5058
                     /gene="pyrI"
                     /locus_tag="RHZ76_000613"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HDV2811306.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            5131..5517
                     /gene="ridA"
                     /locus_tag="RHZ76_000614"
     CDS             5131..5517
                     /gene="ridA"
                     /locus_tag="RHZ76_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HDV2811307.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(5723..8419)
                     /gene="mgtA"
                     /locus_tag="RHZ76_000615"
     CDS             complement(5723..8419)
                     /gene="mgtA"
                     /locus_tag="RHZ76_000615"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDV2811308.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(8560..8613)
                     /gene="mgtL"
                     /locus_tag="RHZ76_000616"
     CDS             complement(8560..8613)
                     /gene="mgtL"
                     /locus_tag="RHZ76_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HDV2811309.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            8798..9745
                     /gene="treR"
                     /locus_tag="RHZ76_000617"
     CDS             8798..9745
                     /gene="treR"
                     /locus_tag="RHZ76_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HDV2811310.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            9864..11285
                     /gene="treB"
                     /locus_tag="RHZ76_000618"
     CDS             9864..11285
                     /gene="treB"
                     /locus_tag="RHZ76_000618"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HDV2811311.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            11335..12990
                     /gene="treC"
                     /locus_tag="RHZ76_000619"
     CDS             11335..12990
                     /gene="treC"
                     /locus_tag="RHZ76_000619"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDV2811312.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDQRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIISRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            13384..15522
                     /gene="nrdD"
                     /locus_tag="RHZ76_000620"
     CDS             13384..15522
                     /gene="nrdD"
                     /locus_tag="RHZ76_000620"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDV2811313.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWSIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            15682..16146
                     /gene="nrdG"
                     /locus_tag="RHZ76_000621"
     CDS             15682..16146
                     /gene="nrdG"
                     /locus_tag="RHZ76_000621"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HDV2811314.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPDKDIW
                     VWTGYKLDDLNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(16191..16577)
                     /gene="cybC"
                     /locus_tag="RHZ76_000622"
     CDS             complement(16191..16577)
                     /gene="cybC"
                     /locus_tag="RHZ76_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HDV2811315.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDTLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(16632..17984)
                     /gene="pmbA"
                     /locus_tag="RHZ76_000623"
     CDS             complement(16632..17984)
                     /gene="pmbA"
                     /locus_tag="RHZ76_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HDV2811316.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGIKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            18078..18629
                     /locus_tag="RHZ76_000624"
     CDS             18078..18629
                     /locus_tag="RHZ76_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HDV2811317.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(18780..20153)
                     /gene="mpl"
                     /locus_tag="RHZ76_000625"
     CDS             complement(18780..20153)
                     /gene="mpl"
                     /locus_tag="RHZ76_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HDV2811318.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESNFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            20329..21327
                     /gene="fbp"
                     /locus_tag="RHZ76_000626"
     CDS             20329..21327
                     /gene="fbp"
                     /locus_tag="RHZ76_000626"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HDV2811319.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(21360..22355)
                     /gene="yjfF"
                     /locus_tag="RHZ76_000627"
     CDS             complement(21360..22355)
                     /gene="yjfF"
                     /locus_tag="RHZ76_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HDV2811320.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIATGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(22342..23364)
                     /locus_tag="RHZ76_000628"
     CDS             complement(22342..23364)
                     /locus_tag="RHZ76_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2811321.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(23378..24880)
                     /locus_tag="RHZ76_000629"
     CDS             complement(23378..24880)
                     /locus_tag="RHZ76_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDV2811322.1"
                     /translation="MTTNQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFDLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETCELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(25020..25976)
                     /gene="ytfQ"
                     /locus_tag="RHZ76_000630"
     CDS             complement(25020..25976)
                     /gene="ytfQ"
                     /locus_tag="RHZ76_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HDV2811323.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            26286..26816
                     /gene="ppa"
                     /locus_tag="RHZ76_000631"
     CDS             26286..26816
                     /gene="ppa"
                     /locus_tag="RHZ76_000631"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HDV2811324.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(27196..27537)
                     /locus_tag="RHZ76_000632"
     CDS             complement(27196..27537)
                     /locus_tag="RHZ76_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HDV2811325.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(27540..31319)
                     /gene="tamB"
                     /locus_tag="RHZ76_000633"
     CDS             complement(27540..31319)
                     /gene="tamB"
                     /locus_tag="RHZ76_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HDV2811326.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDVGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVSTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(31316..33049)
                     /gene="tamA"
                     /locus_tag="RHZ76_000634"
     CDS             complement(31316..33049)
                     /gene="tamA"
                     /locus_tag="RHZ76_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HDV2811327.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            33255..33893
                     /gene="msrA"
                     /locus_tag="RHZ76_000635"
     CDS             33255..33893
                     /gene="msrA"
                     /locus_tag="RHZ76_000635"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDV2811328.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            34216..35559
                     /locus_tag="RHZ76_000636"
     CDS             34216..35559
                     /locus_tag="RHZ76_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDV2811329.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(35621..35827)
                     /locus_tag="RHZ76_000637"
     CDS             complement(35621..35827)
                     /locus_tag="RHZ76_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HDV2811330.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            36152..36709
                     /locus_tag="RHZ76_000638"
     CDS             36152..36709
                     /locus_tag="RHZ76_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDV2811331.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(36699..37439)
                     /gene="cysQ"
                     /locus_tag="RHZ76_000639"
     CDS             complement(36699..37439)
                     /gene="cysQ"
                     /locus_tag="RHZ76_000639"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HDV2811332.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNIMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            37629..39572
                     /gene="cpdB"
                     /locus_tag="RHZ76_000640"
     CDS             37629..39572
                     /gene="cpdB"
                     /locus_tag="RHZ76_000640"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HDV2811333.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYISAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVNKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNNSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPDNTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMVNANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(39695..40075)
                     /locus_tag="RHZ76_000641"
     CDS             complement(39695..40075)
                     /locus_tag="RHZ76_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDV2811334.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            40164..41024
                     /gene="qorB"
                     /locus_tag="RHZ76_000642"
     CDS             40164..41024
                     /gene="qorB"
                     /locus_tag="RHZ76_000642"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001491469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDV2811335.1"
                     /translation="MIAITGATGQLGHYVIKSLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKTAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSETDFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNK"
     gene            41133..42098
                     /locus_tag="RHZ76_000643"
     CDS             41133..42098
                     /locus_tag="RHZ76_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV2811336.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLIRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLTESVSES"
     gene            42206..42868
                     /gene="ytfE"
                     /locus_tag="RHZ76_000644"
     CDS             42206..42868
                     /gene="ytfE"
                     /locus_tag="RHZ76_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HDV2811337.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(42913..44325)
                     /gene="cycA"
                     /locus_tag="RHZ76_000645"
                     /gene_synonym="dagA"
     CDS             complement(42913..44325)
                     /gene="cycA"
                     /locus_tag="RHZ76_000645"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HDV2811338.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(44634..45254)
                     /gene="fklB"
                     /locus_tag="RHZ76_000646"
     CDS             complement(44634..45254)
                     /gene="fklB"
                     /locus_tag="RHZ76_000646"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDV2811339.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLQGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            45473..46111
                     /gene="ytfB"
                     /locus_tag="RHZ76_000647"
     CDS             45473..46111
                     /gene="ytfB"
                     /locus_tag="RHZ76_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HDV2811340.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIILVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(46095..>46437)
                     /locus_tag="RHZ76_000648"
     CDS             complement(46095..>46437)
                     /locus_tag="RHZ76_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDV2811341.1"
                     /translation="WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKH
                     NIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSW
                     LLVDPSSTAHE"
BASE COUNT        11388 a        12362 c        11630 g        11057 t
ORIGIN      
        1 ataactcgtt ataatcaatt gcgtccttcc agttcaactg atgattattt aatagaagcg
       61 ccatattctt gttagcttca cgttggtaac acgccagaac cttgttagta atcaggtgta
      121 atgaactatc atcttttctt tcaaaagact taccttcata acgaaaactc aaaaaatcag
      181 attgtggaaa tataatggct gtatgtcctt gacgagtccc catagtagtg gtagtagaaa
      241 gtgaaatttc tataatcacc ttagatgaat ccgcacgctc accttcagga ttattcataa
      301 caaaattcat tatttcactg ccatcattat ttttgatata agatgaggac tcaatatgag
      361 ccatcatatt tctacccgct tcgttggagc gtttgctaaa atccacataa cccagggtaa
      421 atatttttgc caaaaactcc aaaaatgctc caggagatag aatattcctt ctgctatcct
      481 gcccctcggc attacgagca aaatcattca ttcccacaca ccaggtattt aagacttcat
      541 tttttggttt aacattgtcg gctgatgcca tgtgttgata acaattcaaa tctgatattt
      601 ttgccatgac gaactccttg agttaataag aacgtctaag cattacaaat tttactcaca
      661 aaaaaatatc aaaaatcgct cttttcacaa actcgccgta aacctggagt tatttacggc
      721 gaaaagtcat cacgccatca gcatcttcac tactgctttg cgaacctggg ctggtgcacc
      781 cactgcgcac agcggtttat gcacttcccc cggataaaac acaacaaaat caccttcatt
      841 caggataact gttttctcat caacgacttc cggcaaaaat gcgatgtctt tatctactaa
      901 ccagtcggtc tccggcgtac ctgcaggttg tgtgctgaag gtcatgcctt cctgaccttt
      961 taacacaatc tgaatgtcga gataacgggc atggtactcc gcacggcgag ctttgtacgg
     1021 ctcggtcata tcttccgaga taagataaaa cagacgattg ccttcgatat cgtgcttacc
     1081 ctttggcgtt tctgccgtaa cgtgtgcttt gatatgctca atcgcctggc gtaactcctg
     1141 gggtagccat ggctgaagat tatgaatatt tccgatgatc atcttttcca ctccttaatt
     1201 aaaacaatgt ttcatttcca ccgttatacg aaaagatgat caattccgcc agccttaacg
     1261 cgtgaattat tgtcttatca caacttatcg gcaggcgcgt tcccgccttt cggatcaagg
     1321 tcagcatcac tcctggagaa gagtttttat cgcttccccc gccagtgcag ctgttgcaca
     1381 gagttgttcg agggagtgcc cctctcgtgc cttcgcagaa agccctgaca tcacggtact
     1441 cataaaatca gtcacacatt gtgcgctttg tggatgccgc ctggcgatat agtcataaat
     1501 ggtcgtttct gcggcgtgat aatattgaac ggcaatatca cgcgcttgtg gatcatgact
     1561 atgaatgcct tcaagaacca tacagccagc gcagccgccg ttttggctat atctgcgcgc
     1621 cgcttctttt aatacctcag ccaggcactc ccctaccgga cgatcatcac gaagaatatc
     1681 ggcaagcgga atagcttccg taccgacata ttcattgagt acacggctaa ataacccagc
     1741 cttactgcca aaagccgcgt agagactcgg ggggttaata ccaagataat cagtaacttc
     1801 agcaacactg acagcatcga aacctttttg gtgaaaaagc acttttgccg cagagactgc
     1861 ctgctcagga gcgaaacgtc tgggacgacc tggaacgcga gattgttttt tagtgaccat
     1921 tacaaaactt gttgacagaa agttaaaaca gttttgtaat gcatgttaca taataaatca
     1981 aggagtcctt atgggcgctt ttacaggtaa gacagttctc atcctcggtg gtagccgtgg
     2041 aataggtgcc gctatcgtac gtcgtttcgt caccgatgga gccaatgtac gattcaccta
     2101 tgcggggtcg aaagatgccg ctgaacgcct ggcacatgag actggagcga cagcagtatt
     2161 cacagatagt gctgacagag acgctgttat tgatgtcgtt cgtaagagcg gcgcattgga
     2221 tatcctggtg gtaaatgcag gtattggcgt ctttggcgat gccctggaat taaatgccga
     2281 cgatattgat cgccttttca aaatcaatat tcatgcccct taccatgcct ctgttgaagc
     2341 cgcccggcag atgcccgaag gcgggcgcat cttaatcatc ggctccgtga atggcgatcg
     2401 tatgcctgtt gcaggcatgg ctgcttatgc cgccagtaaa tctgccctgc aaggcatggc
     2461 gcgcgggctg gcccgtgatt ttggaccgcg tgggatcacc atcaacgttg tccagccagg
     2521 gccaatagat accgacgcta atcccgccaa cgggccaatg cgcgatatgt tgcatagttt
     2581 tatggctatc aaaagacatg ggcaaccgga agaggtcgct ggaatggtcg catggttagc
     2641 agggccagaa gccagttttg ttaccggcgc gatgcatacc attgatggcg cgtttggggc
     2701 ataaccgact acgctcaatt aagcccagcc attatccatg atgtctgggt tttgtttact
     2761 cacgtcgttc cctaaaagcg gctcccggta aatataaatc ttctgttcat gtcgtaaatc
     2821 ttgaggataa ccaaatggta gaaagaaccg ctgttttccc tgctggccga cattcactgt
     2881 atgctgaaca tcgttattcg gcggctattc gttccggcga tttgctgttt gtttccgggc
     2941 aagtaggaag tcgagaggac ggaacaccag aacccgattt tcagcaacaa gtcagactgg
     3001 catttgataa tttgcatgcg accctggcag ctgcgggatg cacttttgac gatatcattg
     3061 atgttacgag ctttcatacc gatccggaaa aacaatttga agacatcatg atggtcaaaa
     3121 atgaaatatt tagcgcccct ccttatccaa actggacggc ggtgggcgtt acctggctgg
     3181 caggctttga ttttgaaatt aaagtgatag cgcgcatccc tgagctgtaa gcaatagtgt
     3241 tagccgttcg ttttcacact ccgccgtata agtcggatga atggaataaa atgcatatct
     3301 gattgcgtga aagtgaaaaa gaaaaaagca ggaaatgtct gcaattattg ataccgaacg
     3361 acagttctct tccagaatca aagcaaataa aaatgcatac attttgactt ttaattcaaa
     3421 taaaccgttt gcgctgacaa aatattgcat caaatgcttg caccgcttct ggctatgagt
     3481 ataatgccgg acaatttgcc gggaggatgt atggttcagt gtgttcgaca ttttgtctta
     3541 ccgcgtctga aaaaagacgc tggcctgccg tttttcttcc cgttgatcac ccattcccag
     3601 cccctcaatc gaggggcttt tttttgccca ggcgtcagga gataaaagat ggctaatccg
     3661 ctatatcaga aacatatcat ttccataaac gaccttagtc gcgatgacct taatctggtg
     3721 ctggcgacag cggcgaaact gaaagcaaac ccgcaaccag agctgttgaa gcacaaagtc
     3781 attgccagct gtttcttcga agcctctacc cgtacccgcc tctctttcga aacatctatg
     3841 caccgcctgg gtgccagcgt ggtgggcttc tccgacagcg ccaatacatc actgggtaaa
     3901 aagggcgaaa cgctggccga taccatttcg gttatcagca cttacgtcga tgcgatagtg
     3961 atgcgtcatc cgcaggaagg tgcggcgcgc ctggccaccg agttttccgg caatgtaccg
     4021 gtactgaatg ccggtgatgg ctccaaccaa catccgacgc aaaccttgct ggacttattc
     4081 actattcagg aaacccaggg gcgtctggac aatctccacg tcgcaatggt tggtgacctg
     4141 aaatatggcc gcaccgttca ctccctgact caggcgttag cgaagttcga cggcaaccgt
     4201 ttttacttca tcgcgccgga cgcgctggca atgccgcaat acattctgga tatgctcgat
     4261 gaaaaaggga tcgcatggag tctgcacagc tctattgaag aagtgatggc ggaagtagac
     4321 atcctgtaca tgacccgcgt gcaaaaagag cgtctggacc cgtccgagta cgccaacgtg
     4381 aaagcgcagt ttgttcttcg cgccagcgat ctccacaacg ccaaagccaa tatgaaagtg
     4441 ctgcatccgc tgccgcgtgt tgatgagatt gccaccgatg tggataaaac gccacacgcc
     4501 tggtacttcc agcaggcagg caacgggatt ttcgctcgcc aggcgttact ggcactggtt
     4561 ctgaatcgcg atctggtact gtaaggggaa atagagatga cacacgataa taaattgcag
     4621 gttgaagcta ttaaacgcgg cacggtaatt gaccatatcc ccgcccagat cggttttaag
     4681 ctgttgagtc tgttcaagct gaccgaaacg gatcagcgca tcaccatcgg tctgaacttg
     4741 ccttctggcg agatgggccg caaagatctg atcaaaatcg aaaatacctt tttgagtgaa
     4801 gatcaggtag atcaactggc attgtatgcg ccgcaagcca cggttaaccg tatcgacaac
     4861 tatgaagtgg tgggtaaatc gcgccccagt ctgccagagc gcatcgacaa tgtgctggtc
     4921 tgcccgaaca gcaactgtat cagccatgcc gaaccggttt cttccagctt tgccgtgcga
     4981 aaacgcgcca atgacatcgc gctcaaatgc aaatactgtg aaaaagagtt ttcccataat
     5041 gtggtgctgg ccaattaatt gcggttggta ataaaagtct ggctccctat aatgagccag
     5101 actttttacc gctgtaataa aggagaaatc atgagcaaaa ctatcgcgac ggaaaatgca
     5161 ccggcagcta tcggtcctta cgtacagggc gttgatctgg gcaatatgat catcacctcc
     5221 ggtcagatcc cggtaaatcc gaaaacgggt gaagtaccgg cagacgtcgc tgcacaggca
     5281 cgtcagtcgc tggataacgt aaaagcgatc gtcgaagccg ctggcctgaa agtgggcgac
     5341 atcgttaaaa ccaccgtgtt tgtgaaagat ctgaacgact tcgctaccgt taatgctacc
     5401 tacgaagcct tcttcaccga acacaacgcc accttcccgg cacgttcttg cgttgaagtc
     5461 gcccgtctgc cgaaagacgt gaagattgag atcgaagcga tcgctgttcg tcgctaatct
     5521 tgatggaaat ccgggctatc atgcccggat taagtctgat gacaaacgca aaatcgcctg
     5581 atgcgctacg cttatcaggc ctacgtgatt cctgcaattt attgaatttg ttggccggat
     5641 aaggcattta cgccgcatcc ggcatgaaca aagcttactt tgtctgcaat ctgaatcggg
     5701 gctatcgtgc ccagtttatt ctttattgcc agccgtaacg acggctatag aaccctttca
     5761 ccaactgggt taatgtcata taccctgcca gaatcgcaac cagccacggg aaatagctta
     5821 acggcagcgc ctgtaattgc agataactgg ccagcggtga aaacggcaat gcgatcccga
     5881 caatcatcac gatcacggtc atgatcatta acggccacga tgcacagctc tgaataaacg
     5941 gcacacggcg ggtgcggatc atatgcacaa tcagcgtttg cgacagtaag cccaccacaa
     6001 accatcccga ctggaacagc gtttgcgttt ccggcgtgtt ggcatggaat acccaccaca
     6061 tcaggcaaaa cgtcagaata tcgaagatcg agctgatcgg tccgaagaag atcataaagc
     6121 gccccagatc cgccgggttc caacgctgcg gcttttgaat ttgctcgtcg tcgacgttat
     6181 caaacgggat cgccacctgt gacacatcat acagcaggtt ctgaataagc aagtgtaacg
     6241 gcaacatcgg caggaagggc aagaaagcac tcgccaccag cacgctgaac acattaccga
     6301 agttggagct cgccgtcatt ttgatgtatt tcagcatgtt ggcgaaagtg cgacgtcctt
     6361 caataacccc ctcttccagc accatcaggc ttttttccag caggatgata tcagccgctt
     6421 cacgggcaat atctaccgcg ccgtccacag aaatgccgat atccgcagcg cgtaaggcgg
     6481 gcgcatcatt aataccatcg cccataaagc caaccacatg cccttcgcgc ttcagtaagg
     6541 tcacaatgcg ttctttatgc atcggcgtca gacgagcaaa cagcgtggtg cgctgtgcga
     6601 gatttgccag ttcgtcgtca gatagcgttt caatatcact accaatgacc acctctcccg
     6661 cgtccagccc cacttcatgg cacactttcg ctgcaactaa ctcactgtcg ccagtaagga
     6721 tttttacggt aattccactc gcttttaatg ccttcagtgc cggagcagtt gtctcttttg
     6781 gcggatcaag gaaagcaata tatccttcga ggatcaggtc ggattcatcc gcccgctggt
     6841 aatctccttc acgcgctggc aggtatttcg tcgccaccgc aaccacgcgc agcccctgac
     6901 gattcagcgt atcagtaacc cgcttaatct tacgcagcat gatgtcatcg agcggcacaa
     6961 tctcgccatt gtgacgcacc tgcgaacaca cattgaggat ttcctgcaat gcacctttgc
     7021 aaaccagctg atggtgctcg gtattttctg ccactaccac cgacatccgg cgacgctcga
     7081 aatcaaacgg aatctcatcg attttttgcc aacgactggc cagcgagcgc gctgactctt
     7141 catccgtacc ttcgagcacc gctgtatcga gcaggttttt aagtccggtc tgataatgac
     7201 tgttcaacca cgcgctatgc agcacgcgtt cgctggtttt accggagata tcggtatgat
     7261 tctccagcac aattttgtcc tgtgtcaagg tgccagtttt atcagtgcac agaatatcca
     7321 ttgcgccaaa gttctgaata gcatccagat gtttgacgat cactttctgt ttcgacagct
     7381 ttactgcgcc gcgcgccagc gtcgaggtaa caatcatcgg caacatttcc ggcgttaagc
     7441 ctaccgcgac cgaaagcgca aacagtgccg cttcccacca gtcgcctttg gtgtaaccat
     7501 tgattaacag caccaccgga gccatcacca gcataaagcg aatcagcagc atactgacgc
     7561 ggctgatccc ttgctgaaag gcattcggct cgctttcctg ctcactaaca cgccccgcca
     7621 gttgaccaaa ccaggtattg gcacctgtag caatcaccat tgcttgtgcc gtgccgctca
     7681 ccacggtggt gcccataaaa cacagggtgt cgcactccag cggattgctg tgctccggct
     7741 ggcgagtggt agctgctttt tctacgggca gagactcacc ggttaacgac gcctgagcga
     7801 cgaacagatc ccgcgcctgc aagatacgta aatctgccgg gatcatatcc cccgccgcca
     7861 gttttataat atcgccgggc accagctggt cgatcgggat ctccagccag ccattttcgc
     7921 ctttgtcgtt aattacgcgc agcaccgtcg cagtattgct gaccatcgct ttcagggcat
     7981 ctgccgcttt cgtggaacgt gcttcctgaa taaagttcag caacgtagaa atagcgacca
     8041 ttagcgcgat aacgcccgcg gcaaataaat cttccgtggc gtaagaaata gcgccgagaa
     8101 tggtgagtaa aatattaaat gggttgcgat agcagaccca taaatgtacc caccacggcg
     8161 acggttgttg tgcgggtaat ttattttcac catgttgttc gcgggcagat tccacttccg
     8221 cctgatttaa cccttccgga tgggtgtcga acgttttcca taattcttct tcgggcatca
     8281 ccgccatttt caggcaatgc gcacttaagg acggcgggac cgccgtattc actgtctgct
     8341 gcgcgccagg caatggatcg cgatgaacca gacgggaagg taaatggcga atgagccggg
     8401 taaaaatttc tttaaacata aggagtccct ccgcactgtc tgaaacagta cagaaatgtc
     8461 ttcaggcgtg gcgaagcctt acctgaaggt aataaagctg agcagggacg ttaaaacgtc
     8521 acggccttac gcatccggta acgccgtgaa aagacgggct taccggaaaa gttttaatct
     8581 ccgtcgaggg agaggcgtgg gatcaggttc catataacct ccggtaagtg aaaaaagcgc
     8641 cgcaaataat aaggagaatc caataatatt cgtagcatct atggcgatat aatacctgct
     8701 ggcaactaaa ccaacgataa accagacttt acccattgct gaatgcacgg gtaaagttag
     8761 gctcaaataa ttaaacaaca cgttacagga caacaggatg caaaatcggc tgaccatcaa
     8821 agacatcgca cgcttaagcg gcgtggggaa atctacagtt tcccgggtgc tgaataacga
     8881 aagcggcgtg agccagcgca cccgcgagcg tgttgaagca gtgatgaatc agcatggatt
     8941 ttccccttcc cgctctgcgc gcgctatgcg tgggcaaagc gataaagtgg tcgccatcat
     9001 tgttacccgt ctggattcgt tgtcagaaaa tctcgccgtt caaaccatgc tgccagcgtt
     9061 ctatgaacaa ggttacgacc caatcatgat ggaaagtcag ttttccccac aattagttgc
     9121 cgaacatttg ggggtgctga aacggcgtaa tatcgacggc gtagtgctgt tcggttttac
     9181 tggcataaca gaagaaatgt tagcccactg gcagtcatcg ctggttctgc tggcgcgtga
     9241 cgcaaaaggc tttgcttcgg tctgttatga cgacgaaggg gcaatcaaga tcctgatgca
     9301 acggctgtat gaccaggggc atcgtaatat cagttatctc ggcgtgccgc atagtgacgt
     9361 gacaaccggt aagcgacgtc acgaagccta cctggcgttc tgcaaagcgc ataaactgca
     9421 tcccgttgcc gctctgccag ggcttgctat gaagcaaggc tatgagaacg tggcaaaagt
     9481 gattacgcct gaaactactg cgttactgtg cgcaaccgac acgctggcac ttggcgcaag
     9541 taaatacctg caagagcaac gcatcgacac cttgcaactg gcgagcgtcg gtaatacacc
     9601 gttaatgaaa ttcctccatc cggagatcgt caccgtagat cccggttacg ccgaagctgg
     9661 acgccaggcg gcctgccagt tgatcgcaca ggtaaccggg cgcagcgaac cgcaacaaat
     9721 catcatcccc gccaccctgt cctgatcgtt tcctgaacga taaattgtga tcttcgctgc
     9781 gtttcgggaa cgttcccgtt tttaaatttt tccgcgcaat atattctgca gccaaccaaa
     9841 aatgtcatct gccatggggc tttatgatga gcaaaataaa ccaaacggat atcgatcggt
     9901 tgattgaact ggtcggcggg cgcggcaata ttgcgacggt gagccactgt attactcgcc
     9961 tgcgctttgt cctcaaccaa ccggccaatg ccagaccgaa agaaattgag caactcccca
    10021 tggtgaaagg ctgtttcacc aatgccgggc aatttcaggt ggtgattggc accaatgtgg
    10081 gtgattacta tcaagcactg attgcgtcaa ccggacaggc gcaggttgat aaagagcagg
    10141 taaaaaaagc tgcccggcag aatatgaaat ggcatgagca gttgatctct catttcgcgg
    10201 agatcttctt cccgttgctg cccgcgttga ttagcggcgg tttgatcctc ggttttcgca
    10261 atgtgatcgg cgatttgccc atgagcaacg gtcaaacgct ggcgcaaatg tacccttccc
    10321 tgcaaacgat ctacgatttt ctgtggttga tcggtgaagc gatcttcttc tacctgccgg
    10381 tcgggatttg ctggtcagcg gtgaaaaaaa tgggcggcac gccgatcctc ggtatcgtgc
    10441 ttggcgtgac actggtttct ccacagctga tgaacgctta tctgctcggg cagcagctgc
    10501 cggaagtgtg ggactttggc atgttcagca tcgccaaagt aggctatcag gcgcaggtga
    10561 tcccggcact gttagccgga ctggcgctgg gcgttattga aactcgcctt aaacgcatcg
    10621 tgccggatta cctctatctg gtggtggtac ccgtctgttc gctgatcctc gcggtgttcc
    10681 tcgcccatgc gctgattggt ccgtttggtc gcatgattgg cgatggcgtt gcctttgcgg
    10741 tacgtcacct gatgaccggc agctttgctc cgattggtgc ggcattgttt ggcttcctgt
    10801 acgcgccgct ggtgatcacc ggcgtacacc agaccaccct tgctattgat ttgcagatga
    10861 ttcaaagcat gggtggcacg ccagtgtggc cgctgattgc gctgtcgaat atcgctcagg
    10921 gctccgccgt gataggcatt atcatttcca gccgcaagca caatgaacgc gagatctccg
    10981 tgcctgccgc tatctccgcc tggcttgggg tcactgagcc tgcaatgtac ggcatcaacc
    11041 tgaaatatcg cttcccgatg ctgtgcgcga tgattggttc tggtctggca ggattgctat
    11101 gcggcctgaa cggcgttatg gcgaatggca tcggcgtagg cggcctgccg ggaattctct
    11161 cgattcaacc gagctactgg caggtatttg cgctggcaat ggctatcgcc atcatcatcc
    11221 cgattgtact cacctcgttt atctatcagc ggaaataccg cctgggcacg ctggacattg
    11281 tttaattttc ttcggggcgc aattgcgccc cctcgcattt gcaggaataa cgtaatgact
    11341 aatcttcccc actggtggca aaacggcgtt atctaccaga tttatccaaa gagttttcag
    11401 gacaccacgg gtagcggcac cggcgattta cgtggcgtta tccaacgcct ggactatctg
    11461 cataaactgg gcgttgatgc catctggcta accccctttt atgtctctcc ccaggtcgat
    11521 aacggttacg atgtagcgaa ctatacggcg attgatccca cctacggcac gctggacgat
    11581 tttgacgaac tggtgacgca ggcaaaatcg cgcgggattc gtatcattct cgatatggtg
    11641 tttaaccata cctctaccca acatgcctgg tttcgcgagg cgctgaacaa agaaagccct
    11701 taccgccagt tttatatctg gcgcgatggc gaaccagaaa cgccaccgaa caactggcgt
    11761 tcaaaatttg gcggtagtgc gtggcgctgg catgcggaaa gcgaacagta ctatttgcat
    11821 ctctttgcac cagaacaggc ggatctcaac tgggagaatc cagcggtacg cgcagagctg
    11881 aaaaaagtct gtgaattctg ggccgatcgt ggggtcgacg ggttgcgcct ggatgtggtg
    11941 aatctgatct ccaaagacca gcgtttccct gaagacctgg atggcgacgg gcgtcgcttc
    12001 tacaccgacg ggccacgagc acacgagttt ttgcacgaga tgaaccgcga tgtgtttacg
    12061 ccacgcgggt taatgaccgt aggtgaaatg tcctccacca gccttgagca ttgccagcga
    12121 tacgcggcac tgacaggcag tgaattgtcg atgaccttta attttcatca cctgaaggtc
    12181 gattatcccg gtggtgaaaa atggacgctg gctaaacctg actttgtggc gttgaaaaca
    12241 ttgttccgcc actggcaaca aggaatgcac aacgtggcat ggaatgcctt gttctggtgt
    12301 aaccacgatc agccgcgcat tatttctcgc tttggtgatg aaggtgaata ccgcgtgcct
    12361 gcggcaaaaa tgctggcgat ggtgctgcat ggcatgcagg gaacgccgta tatctaccag
    12421 ggcgaagaga ttggcatgac caacccgcat ttcacgcgca ttactgacta tcgcgacgtg
    12481 gagagcctca atatgtttgc cgagctgcgc aacgatggtc gtgatgccga cgagttattg
    12541 gcaatcctcg ccagtaaatc ccgtgacaac agccgcacgc ccatgcaatg gagcaacggc
    12601 gataatgccg ggtttacggc tggcgaaccg tggattggcc tgggcgataa ctatcaacaa
    12661 atcaacgtag aagccgcgct ggccgatgag tcctcggtgt tttacaccta ccaaaagtta
    12721 atcgcactgc gtaagcaaga agccatcctg acatggggca attaccagga tctgctgcca
    12781 aacagccctg tattgtggtg ctatcgccgt gaatggaagg ggcaaacctt gctggtcatt
    12841 gccaacctta gccgtgagat ccaaccctgg cagccagggc aaatgcgcgg caactggcag
    12901 cttgtgatgc ataactacga agaagcctca ccacaaccct gtgccatgaa tttacggcct
    12961 tttgaggctg tctggtggtt acagaagtaa atcttcccta agccccggta atgccggtca
    13021 ttccggggtt ttgtctgtca gcatgttgtt tttgttgatt tttcaaccag caaattcatt
    13081 aaaaaattta cataacgctg tagcgcccgt catccgtacg ctctgctttt tactttgagc
    13141 tacatcaaaa aaagttaaaa catccttgat gcaaagcact atatatagac tttaaaatgc
    13201 gtcccaaccc aatatgttgt attaatcgac tataattgct actacagctc cccacgaaaa
    13261 aggtgcggcg ttgtggataa gcggatggcg attgcggaaa gcaccggaaa acgaaacgaa
    13321 aaaaccggaa aacgcctttc ccaatttctg tggataacct gttcttaaaa atatggagcg
    13381 atcatgacac cgcatgtgat gaaacgagac ggctgcaaag tgccgtttaa atcagagcgc
    13441 atcaaagaag cgattctgcg tgcagctaaa gcagcggaag tcgatgatgc cgattattgc
    13501 gccactgttg ccgcggttgt cagcgagcag atgcagggcc gcaaccaggt ggatatcaac
    13561 gagatccaga ccgcagttga aaaccagctg atgtcgggtc catacaaaca actggctcgc
    13621 gcttacatcg agtaccgtca cgatcgcgac attgaacgtg aaaaacgcgg tcgcctgaac
    13681 caggagatcc gtggtctggt cgagcagacc aacgcctcgt tactcaacga aaacgccaac
    13741 aaagacagca aggtgattcc aacccagcgc gacctgctgg ccgggatcgt ggctaaacac
    13801 tatgcacgtc agcacctgct gccgcgtgac gtggtgcagg cacatgagcg tggcgatatt
    13861 cactatcacg atctcgatta ctcaccgttt ttcccgatgt tcaactgcat gttgatcgac
    13921 ctgaaaggca tgctgaccca gggctttaaa atggggaacg ccgagattga accgccgaag
    13981 tcgatctcaa cggcaaccgc ggtaactgcg cagattattg ctcaggttgc cagccatatt
    14041 tatggcggca ccaccattaa ccgtatcgat gaagtgctgg caccgtttgt caccgccagc
    14101 tacaacaaac atcgcaagac cgcagaagag tggagcatcc cggacgcaga aggctacgct
    14161 aactctcgta ccattaaaga gtgctacgat gccttccagt cgctggagta cgaagtaaac
    14221 accctgcata ccgccaacgg tcaaacgccg tttgtaactt ttggttttgg tctgggcacc
    14281 agctgggaat cgcgcctgat tcaggaatct atcctgcgta accgtatcgc aggtctcggt
    14341 aaaaaccgta aaactgcggt gttcccgaaa ctggtgtttg cgattcgcga tggtctgaac
    14401 cataaaaaag gcgatccgaa ctacgacatc aaacagctgg cgctggagtg cgcaagcaag
    14461 cgcatgtatc cggatatcct gaactacgat caggtagtga aagtcaccgg ttcgtttaaa
    14521 accccgatgg gctgccgcag cttcctcggc gtgtgggaaa atgaaaacgg cgagcagatc
    14581 cacgatggtc gtaacaacct cggcgtgatc agcctgaacc tgccgcgtat tgctctggaa
    14641 gcaaaaggcg atgaagccac cttctggaag ctgctggatg aacgtctggt gctggcacgt
    14701 aaggcgctga tgacccgtat cgctcgtctg gaaggcgtga aagcgcgcgt ggccccgatc
    14761 ctttatatgg aaggtgcttg tggcgtgcgt ctgaatgctg acgatgatgt ttctgaaatc
    14821 ttcaaaaacg gtcgtgcgtc tatttcgctg ggttacatcg gcatccacga aaccattaac
    14881 gcgctgttcg gcggcgagca tgtttacgac aacgagcagc tccgcgcgaa aggtatcgcg
    14941 attgttgaac gtctgcgtca ggcagtggat cagtggaaag aagaaacggg ttatggtttc
    15001 agtctctaca gcacgccgag tgaaaacctg tgcgaccgct tctgccgtct cgatactgct
    15061 gagtttggcg tggtgccggg cgtgaccgat aaaggttact acaccaacag tttccacctt
    15121 gatgtggaga agaaggtgaa cccgtacgac aagatcgact ttgaagcgcc ttacccgccg
    15181 ctggcgaacg gtggtttcat ttgctacggc gagtatccaa acattcagca caacctgaaa
    15241 gcgctggaag atgtctggga ttacagctat cagcatgtac cgtattacgg caccaataca
    15301 ccgattgatg agtgctacga gtgtggcttt accggtgagt tcgagtgcac cagcaaaggc
    15361 ttcacttgcc cgaaatgtgg taaccatgac gcctcccgtg tgtcggtaac tcgccgcgtg
    15421 tgcggatatt taggtagccc ggatgcacgt ccgtttaacg ctggtaagca ggaagaagtt
    15481 aagcgccgcg ttaaacattt ggggaatggg cagataggtt aatcttcact ctgcgctaag
    15541 caaatgccgg atgcggcgta aacgccttat ccggcctaca tttggcaacg gctgtgtagg
    15601 cctgataaga cgcgacagcg tcgcatcagg cattgattac cggatgcagc ctacacgcgg
    15661 actgataagc caggcgaaat aatgaattat catcagtact atcctgtcga catcgtcaac
    15721 ggccccggca ctcgctgcac cctgtttgtc tccgggtgtg ttcatgaatg ccccggttgc
    15781 tataacaaaa gcacatggcg ggtaaattct ggtcagccat ttaccaaagc aatggaagac
    15841 cagatcatta acgatctgaa tgacactcgt atcaaacgcc aggggatttc cctctccggc
    15901 ggcgatccgc tgcatccgca aaacgtgccg gatattctga aactggtaaa acgcatccgc
    15961 gccgagtgtc cggataaaga catctgggtg tggacaggct ataaactcga cgatctcaac
    16021 gctgcgcaaa tgcaggttgt tgatttgatt aacgtactgg tcgacggcaa atttgtgcag
    16081 gatttaaaag acccttccct gatctggcgc ggcagcagca atcaggtggt gcatcatttg
    16141 cgatgataag ttccctgtta cctgagtgca ggcaacaggg aaatgaggaa ttaacgatac
    16201 ttctggtgat aggcgttgcg ggtcgttttc agttgctctg cggcagcctg cgcttctttt
    16261 actttacctt catttgccag cttcagcgcg tcgtcaatct gaccaaccag aatatcgaaa
    16321 ccgtgacgga agtctttcat ttccgggctg tccggtgatt tatcttcgag cttcggcggc
    16381 gttgcttttt gcgcatccag cgctgcggcg cgcatcttcg ttaacgcgtc tttgacttgc
    16441 gccgcgttat cggctttttc gaccactttt aaagtgtcgt tgagggtttc catattgtct
    16501 tcaagatcag cggcaaacga cgccgaactg aataccaacg aggagactgc aagaatagct
    16561 aacaggcttt tacgcattgc tcacttcctt ttttattatg aagatttggc gcggcaggtc
    16621 gcgccagttt tttactgccc ggcgattttc atctccggca acagcacaga accacactgt
    16681 atattactgc gtgtttcaat atcgttaccg acggtgacaa tattgcgcca catatctttt
    16741 aaattacctg cgatggtgat ttcgctaacc ggatactgaa tttcgccgtt ttctacccag
    16801 aagcccgccg caccacggga ataatcaccg gtaattgcac tcacgccctg gcccatcagc
    16861 tccgtcacca ccagcccggt gcccatctct ttgagcattt gttcgaagct tagcccttgt
    16921 ccggcaatcc gccagttgtg aataccgccc gcatgtccgg tgcttttcag ccccagtttt
    16981 cgcgccgaat agctggtcag cagccactga gtcaggatgc catctttaat aatatcgcga
    17041 cgctcggtgc gcacaccttc gctgtcgaat ggcgtcgacg ccagcccttt cagcagatgc
    17101 ggatgctctt caatggtcag ccagtccggc agaatttgtt tacccagcga atccagcaga
    17161 aaggtagatt tacggtaaac cgagccaccc gctatcgccc ccaccagatg gccaaaaagc
    17221 ccggttgcca cttcattggc aaaaatgacc ggcgctttca tggtggagag tttacgcggt
    17281 gacaggcgcg ataaagtacg gcgagcacag tcggccccaa cccactctgg cgtttgcaga
    17341 tcgctcatcg cacgaccaat ggtgtaggcg taatcacgct ccatatcgcc attttcttcg
    17401 gcaattacac agctggagag cgaatgacgc gttgagcagt aaccctgcaa catgccgtgg
    17461 ctgttgccaa aaactttgat accgtagtgg ctgttaaagc tgccaccttc ggtattggtg
    17521 atgcgtttgt ccgcctgcaa tgccgcctgt tctgcgcggg ccgccagttc aatggcttca
    17581 tccggggaaa cttccgcagg gtggaacaag tcgagatctg gtgcgtcaaa ggccagcagc
    17641 tctttgtctg ccacgccggc acagggatct ggcgaggtat aacgggcaat atccagcgcc
    17701 gcctgtacag tacgggcaat ggcctgcggg cttaaatcgg tggatgatgc gctacctttg
    17761 cggttctggt gataaacagt gatccccagc gcgccatcgc tattgaattc aacattctcc
    17821 acttcaccat aacgcgtgct tacgctaatg ccggtggtct tgctgacggc aacttccgca
    17881 ccgtccgatt tgcctgaggc caactccagc gcagtggaaa ctgcttcttc cagaatcttg
    17941 cgctgcgctt caacttgaga gattactttc attgcaagtg ccataatgta gagagtttct
    18001 ctgaagtcta acagagaacc gtttttcagt gcgcatctta actggtaaca ttagcctctt
    18061 tttttaagga gcctgagatg actaagcagc ccgaagactg gctcgacgac gttcccggtg
    18121 atgacatcga agacgaagac gatgaaatta tctgggtcag taaaagtgaa attaaacgtg
    18181 atgccgagga gctaaaacgc cttggcgcgg aaattgttga tctggggaaa aacgcgctgg
    18241 ataagatccc gttagatgcg gatctgcgcg cggctattga gctcgcccag cgtattaaga
    18301 tggaaggtcg ccgccgccag ttgcagctca tcggtaagat gctgcgccag cgcgatgtag
    18361 aaccgatccg tcaggcgctg gataagctga aaaaccgtca caaccagcag gtggtgctgt
    18421 tccataaact ggaaaacctg cgcgatcgcc tgatcgatca aggtgatgac gccatcgctg
    18481 aggtattaaa tctgtggccc gatgccgatc gtcagcaact gcgtactctg atccgtaacg
    18541 cgaagaaaga gaaagaaggg aataagccgc cgaaatccgc acgccagatt ttccagtatc
    18601 tgcgcgaact ggcggagaat gaagggtaat tttcgttgtt gatgtgagta tggtttgccg
    18661 gatgcggtgc acctgtaggc ctgataagac gcatcaagcg tcgcatcagg cactatgctc
    18721 aaatgccgga tgcggcgtga acgccttatc cggcaatgct atctcaggct gaggccgaat
    18781 tactgcgcag cttccgcctt cttcgccaga ccatccagca gtttctgatg gatcccacca
    18841 aaaccgccgt tgctcatcac cagaatatgg tcgccaggct gagcggtttt caccaccata
    18901 tctgccagcg tatccacatc gccactccag tgtgcaggct gaacgcaggc ttctgccact
    18961 tctgccacct gccacggaat atgcgccggt tgcagcagga agacttcatc ggcacgacct
    19021 aatgaaggtg ccagatcgtc tttgcagatc cccattttca tggtattcga gcgcggttcc
    19081 agcacagcaa taatgcgcgc cgtaccacca actttgccac gcagcgccgc cagcgttgcc
    19141 agaatcgccg tcgggtgatg ggcaaaatcg tcatataccg tgacgccatt cgcttcacca
    19201 cgcaactcca gacggcgacg agcattaata aacgaaccca gcgcgttagc ggcatctgcc
    19261 ggcgctacac caacatggcg agccgctgca atcgccatca ggccattgtg catattatgt
    19321 tcgcctacca gcgaccattt cacttcgccc actttttcgc catccagcaa aacttcccat
    19381 tcggaagcat cggtggtcag ctttttcgcc tgccagtgac cctgctcgcc caccagctcc
    19441 tgctcgctcc agcagcccat cgccatggtc tgtttcaggt tgatgtcgtt ttctggccag
    19501 ataatacggc cctgccctgg aacgatacgc accagatggt ggaactgttt ctggatcgct
    19561 ttcaggtcgt caaagatatc ggcgtgatcg aactcaaggt tgttgaggat cagcgtacgc
    19621 gggcagtaat gaacaaattt agagcgtttg tcgaagaagg cgcagtcata ctcatccgct
    19681 tcgataacaa agaagttgct ttcgcccaga cgcgccgaaa cctcaaagtt ccccggcaca
    19741 ccgccgatca caaatcccgg tttgtaaccg cactgttcca gaatccaggt cgccattccc
    19801 gcggtggtgg ttttgccatg tgtaccggca acggccagca cccagcggtc gcgcagcaca
    19861 aaatcgtgca gccactgtgg acctgacata taagggatgt ttttttccag taccgcttcc
    19921 acacacggat ttccacgggt catggcgttg ccaataatca ccagatccgg ctgcggatcg
    19981 agctggctgg catcgtaacc ctgaatcagt tcaatgcctt gcttctcaag taaggtgctc
    20041 atcggcggat acacattggc gtccgaaccc gttacttcat ggcctaactg gcgcgccagc
    20101 atcgccagac cgcccataaa cgtgccacaa attcctaaaa tatgaatgcg catacgtcac
    20161 tatccttttt taatctgccg ctcattttac gcatatgtcg ggcaagtgag aaacgcattt
    20221 caggataatc cgcaatttgc tggcgcgatt cacctgagcg caacattccg attatttgtt
    20281 aagattgtta cggtcgcttt actccataaa cattgcaggg aaagttttat gaaaacgtta
    20341 ggtgaattta ttgtcgaaaa gcagcacgag ttttctcatg ctaccggtga gctcactgct
    20401 ttgctgtcgg caataaaact gggcgccaag attatccatc gcgatatcaa caaagcagga
    20461 ctggttgata tcctgggtgc cagcggtgct gagaacgtgc agggcgaggt tcagcaaaaa
    20521 ctcgacttgt tcgctaatga aaaactgaaa gccgcactga aagcacgcga tatcgttgcg
    20581 ggcattgcct ctgaagaaga agatgagatt gtcgtctttg aaggctgtga acatgcaaaa
    20641 tacgtggtgc tgatggaccc actggatggc tcgtccaaca tcgatgttaa cgtctctgtc
    20701 ggtaccattt tctccatcta ccgccgcgtt acgcccgtcg gcacgccggt aacggaagaa
    20761 gatttcctcc agcctggtaa caaacaggtt gcggcaggtt acgtggtata cggctcctct
    20821 accatgctgg tttacaccac cggatgcggt gttcacgcct ttacttacga tccttcgctc
    20881 ggcgttttct gcctgtgcca ggaacggatg cgcttcccgg agaaaggcaa aacctactcc
    20941 atcaacgaag gaaactacat taagtttccg aacggggtga agaagtacat taaattctgc
    21001 caggaagaag ataaatccac caaccgcccg tatacctcac gttatatcgg ttcactggtc
    21061 gcggatttcc accgtaacct gctgaaaggc ggtatttatc tctacccaag caccgccagc
    21121 cacccggacg gcaaactgcg tttgctttat gagtgcaacc cgatggcatt cctggcggaa
    21181 caagcgggcg gtaaagcgag cgatggcaaa gagcgtattc tggatatcat cccggaaact
    21241 ctgcaccagc gccgttcgtt ctttgtcggc aacgaccata tggttgaaga tgtcgaacgc
    21301 tttatccgtg agttcccgga cgcgtaatct cctgcgcctc tccacacgga gaggcgtttt
    21361 tatcgctgcg caatgttgac tcttgtcact ggcgaactct gacgattctc ccacagcacc
    21421 gttaatccac gctgtaatgc tataaaaata aacaacaaaa taccggtggc gattttcgtc
    21481 caccatgagc tcagcgtgcc atcaaagttt atgtaagtct gaatcagtcc ctgaatcgcc
    21541 acgccaaaaa gcgtccctaa taccgttcca acgccaccgc tcagaagcgt accgccaatt
    21601 accactgagg cgatagcatc cagttccaca cctacgcccg ccagcgcata tccggcctgg
    21661 gtataaatcg agaagacaat ccccgccagc gttgccagtc cggtggagag catataaatg
    21721 cgaatagtgg tgctgcgagt ggaaatcccc atcaggttcg ccgacgttgc gttgccgcca
    21781 atggcgtata cctgattacc aaaacgggta cgatgcgcga ggaaaatacc gataaccacc
    21841 accgccaaca tcagcagtcc catcgcactt aagcgaccgc cgccagggat tttccacgca
    21901 aggcttgaga gcgtgtcata aatgggatgg tttatcggaa tcgactcttc cgaaacgaga
    21961 tagctgacac cgcgcaaaaa gaacatcccc gccagggtaa tgataaatgc cgggatcttc
    22021 agggcgtcga tcaaaagccc cataaatgcg ccgaaggcac agcccatcac cagcactagc
    22081 ggaaacgcca gtagcggcga gaggccgaaa tcgccaatca cttttgccag aaacacgcca
    22141 gtaaaggcga tcaccgaacc gacggagaga tcgatcccac cggagaggat cacaaaggtc
    22201 atgccaacgg caatgatccc aagaaaggcg ttatcggtca ggatattgca gatcactctt
    22261 gtggaagcaa aaccgggaaa ctgggtcagg cagtaaagat aacccaacac aaagacgccg
    22321 atggtgatca tcagtggcaa attacgtttt atcacggcta cgtactcctt taatcagact
    22381 gataaagcgt tgcgactgga caatcagcac gcaaagaacc accaccgctt ttacaacctg
    22441 gttcatctct ggcggaaagc ccgaaagcaa aattccggtg ttcatcccct gaataatcag
    22501 cgcccccacc accgaaagca gtaggttaaa acgtccgccc atcagcgatc cgccgccaat
    22561 aaccaccgcg agaatggcgt ccagctccag ccataacccg gcgttattgg catcggcacc
    22621 gcgaatatcc gccgccacga taatgcccgc aatcgccgca cacagcccgc tcaacacgta
    22681 ggtaagcatg acgatgatcc gcgtgtttac cccggcattt tttgccgccc gaatgttgat
    22741 accaacggct tcgataaaca tccccagcgc cgttttgcgg gtcaacagcc agaacaggat
    22801 aagcgtcagc accgcgataa tgaccggcgt tggcaggaac aacagcgatc cactgccaaa
    22861 ccatgagaga tccggcgagt taaacgtgac gatctgtccg gaggtgatca gttgcgccac
    22921 gccgcgcccg gcgaccatca ggatcagggt ggcaacaaac ggctgaattt tgaggatcgc
    22981 taccagtatg ccgttccaca atcccgccag gatgccagtg cccagggcgc ttaacaaaac
    23041 aatcggcagg ctgaatcccg cgaccgtcat cgcagccgtt gtggctccgg cgatagccat
    23101 caccgccccc acggagagat caatcccacc tgttgcgatc accagcgtca ttccgatagc
    23161 caacagcgca acgggggccg cacggttaag aatgtctatg gggctaccga ataaacgccc
    23221 atcctggagc accacctgcc agaaatgcgg ggccaccagg ctatcgacca gcagcaccag
    23281 caacagtgcc gccagctgcg gcattcccgt tggccagcga aagcgccttt tcggcggcgt
    23341 agtgtccggg agagattgag gcatcacact gttctcctta cgccgcaatg gcgttcatga
    23401 tcgccggaac ggaaagctct gccagcggga tctccgccac ctgtttgcga tcgcgcatga
    23461 tgatcacccg gtcggcatag cccaccagtt cttccagttc ggaggagatc accagcagcg
    23521 ccagaccatc ggcgcatagc gtttcaatca ggcggatgat ctcggcgtgg gcaccaacat
    23581 caatgccgcg agttggctca tcaaggatca gaaattgcgg tcgggtcagt agccaacgtg
    23641 aaagcaacac tttttgctga ttgccgccag agagaaattc aatcggttgt tcagttgaag
    23701 gtgtgcgaat gccaagctgg cggataaagc gttcggcaat ctcttgctgt tctttgcggg
    23761 aaatgggacg tagccagccg cgctgggcct ggagagcgag gatgatattt tcccgcaccg
    23821 aggcggcagc gatgatgcca tcggttttcc tgtcttccgg gcagaagcca atgcccagta
    23881 ccgaagcctg atgtggcgat cgcaggtttt gcggtttgcc tttgatcaac gccgtgccgc
    23941 tgtcagcagg tttgataccg aagatcactt cggcggtttc ggtacgtcct gatcccagca
    24001 atccagccag accgacgatc tcgccggggc gtacttcgag atcaaacggt gcgatcgttc
    24061 cttttttgcc gtaatttttg aacgcggcaa cgggtttgtc gctcaacaat gttcgcccgg
    24121 cacgctgtag cgcgtgggta tccagctcgc gccccagcat catttttacc agttcgatct
    24181 gcggtagctc gcacgtttcc cgacagccta cgaaactgcc gttgcgtaag acggtgatcc
    24241 gatcgctgac ctgatagacc tgatcgagaa agtgagtgac aaaaatcagg ctgacgccgc
    24301 gatcgcgcaa ctgacgcatc aggtcaaaca gtaactccac ttcctgggtg tcgagactgg
    24361 cggtgggttc atcgaggatc agcactttgg cagagagatc gatagcccgg caaatagcga
    24421 cgatttgctg catcgcgact gaaaagcggt tgagcggttc gcgcacgtcg agggagaaac
    24481 cgtaagatgc catcagttcg gtggcgcgct tttccatctc tttgcggcgt agaaggccga
    24541 agcgtttggg ttcgcggcct ataaatagat tatcagcgac cgacatattg ggtagcaggt
    24601 ttacttcctg atagacggtg ccgataccga gttgttgtgc atgggcggta tttttcggtg
    24661 agatagcctg gccttccagc cagatggtgc cgcgatcggc gtggtataca ccagttaatg
    24721 ctttgattag cgttgatttt cctgccccgt tttcaccgag cagcgccatg atttcgccgc
    24781 ggcgcaggct gaaatcaacg ttgtctaacg ctttgacgcc ggggaaaaat ttacttaatc
    24841 cttcggtgcg gaggatctcc tggtgttggt tagtcgtcat ggtttccccc ggtgtaaagc
    24901 gtgctcccag taggcatgat aagacgcggt aagcgtcgca tcaggcttca gcgcaccacc
    24961 gccggatgcg gcgcgagcgc cttatccggc ctacgaatcg gcatcaggca tagcaaccct
    25021 caatacccca tatttttctt cttctctaac tcttcttttg cggtatcagg cagataaagg
    25081 gtggatttgg tcagcgtcag cttttcaggc atggtgccgt cttttttgta tttctccagc
    25141 gcgtcgaagg cgggacctgc catattcggc gtcagttcaa cactggcgtt cgcttcgcca
    25201 tcaatcatcg ctttgtagat atccggtacg ccgtcgatag aacctgtcag aatatctttg
    25261 cctggtttca ggcccgcttc tttaattgcc tgaattgcac caatcaccat gtcgtcgtta
    25321 tgggcgtaaa ccatgcagat gtttttgccg ttgttttccg ctttgataaa gctctccatg
    25381 acttctttgc ctttactgcg ggtgaagtca cctgactgcg agcggataat tttgatattt
    25441 ggcgcattct taatggcttc ggcaaagcct ttcttacggt caatggcgac gctggcccca
    25501 acagtgccct gcagctccac cacgttgcat ggtttgccat tcacttcttt taccagccag
    25561 tcaccaatca acttgccttc gagaatgttg tcggcagtga cggtggtcat atagagagat
    25621 ttgtctttca catcaataga acgatcgagc aagaagaccg ggatttcggc atctttcgcc
    25681 tcttttaata ccggttccca accagtcgca accaccggag cgataaagat cgcatccacc
    25741 ccttgcgcga cgaaggaacg taccgcttta atctggtttt cctgcttttg ctgaccatcg
    25801 gcaattttca gcgtaattcc gcgtttttcg gcctcacttt tcgccacatt ggtttctgcg
    25861 gcgcgccagc cagattccga tccgacctgc gaaaatccta cggttaatgg agcggccaac
    25921 gccatagacg acatggctgc cgagactgca gagactacaa gtaagcgttt ccacataggg
    25981 ttgtcctcgt cggggtaatt attattggtg aaaagatgtt cgcgaaaaaa ctatagacaa
    26041 ttcgttatgt aacggattgc gttacatcac acttcaaaga agtaaataaa acgttaatca
    26101 caagtttgta atcgctttca tctcactatg aaaaatgcgg ctacggttat ggattttcct
    26161 gctctgtata ccgtcttaaa attggcgaaa aaggaaaatg aagacgaaaa caagcgaaga
    26221 cattcggcgc gagttggcta taatactcgg cacttgtttg ccacatattt ttaaaggaaa
    26281 cagacatgag cttactcaac gtccctgcgg gtaaagatct gccggaagac atctacgttg
    26341 ttattgagat cccggctaac gcagatccga tcaaatacga aatcgacaaa gagagcggcg
    26401 cactgttcgt tgaccgcttc atgtccaccg cgatgttcta tccgtgcaac tacggttaca
    26461 tcaaccacac cctgtctctg gacggtgacc cggttgacgt actggtcccg actccgtacc
    26521 cgctgcagcc gggttctgtg atccgttgcc gtccggttgg cgttctgaaa atgaccgacg
    26581 aagccggtga agatgcgaaa ctgattgctg ttccgcacac aaagctgagc aaagaatacg
    26641 atcacattaa agacgttaac gatctgccag agctgctgaa agcgcagatc gctcacttct
    26701 tcgagcacta caaagacctc gaaaaaggca agtgggtgaa agttgaaggt tgggaaaacg
    26761 cagaagccgc taaagctgaa atcgttgctt ccttcgagcg cgcaaagaat aaataagttc
    26821 ttctagcgca ataaccctga acgccgggct tcggttagta agggtttttt tatgcccgcg
    26881 ataaataaac tctctattcc accatcatta ttctcagcgg ttgcaaggct tgaacggtaa
    26941 gaacaagcaa acccgaccac cattttgctg ttcatagcca cttgctggaa gttagccgac
    27001 ctcactcata ctcaccgttc aaagcgtgcc cccgttttac ccctgcacat ttgtgaacgc
    27061 cagcagagtt ttttatgcga ttgcagatat ggaaagcggc tcggagagaa aagcaaaaga
    27121 tggaaaagtt acaaaaaaaa atcgcgagat acatcgcata aaacaacgcc acccgaaggt
    27181 ggcgtgcttt tattattact tatccctgtc taaccagtcg ccgctttcaa ttagctttaa
    27241 tccatcgacg ggtcgttgat aaacgtacat ccatgcactc ccgtacggcg tctgaatcaa
    27301 ctggcgcgcg tattcaccgc ccctggtgcg caaggcatca agttcggcca gcgtggcgtt
    27361 gtcaatacga taaacttcac cgtgtactgt tccgttcccc ggaactgcgc ctggatagtg
    27421 gcccaggcta tacaactggt agttatcgat actgaaatcg cccagtaact gggcattggt
    27481 catccagtga ctgttgcctt gtttgtggcg taaactgccg tagacaaata ttcgcattgc
    27541 taaaactcga actgatagag caaatccagt gcctggtcta caccagacac ggcttccaga
    27601 tatagcttag gcatcaggcg ataacgtaac gtgagtgttg ctatagagtc aaatataccc
    27661 acgccgtatt tcacttgcag acctggcaat acatagccgc tgaccactac ctgggaggag
    27721 tcgcctactc cctgggtgtc gagcgctaaa ttgcttacgc caaacgtctc gccgatttta
    27781 cccacaatct ggccactttg cgcaaccccc agaccaatca gcatcgaggt cattgccgca
    27841 ctgtcgctct gatcgctctc cagcccttgt ccacgtagca aataagacaa tgcagcttgt
    27901 tgcgacatcg ccgggtcaga gaagatctcc gctttcggtt cgtccgccag accagtgacg
    27961 cgaactccgg cgattacgtc gtcttctgta gcatccgggt tacgaatagc ttcaatatta
    28021 agatacggtt gatccggcgg accagagaac agtaactcac ctttacgcac aatcagatcc
    28081 tgaccatagg catggaagcg cccttcaggg atgttgatct gcccgttcag gcccagccct
    28141 tgtttgtcct gaacgacgtt aagatcgccc gtcagccgcg ctttcaggcc aaaggcgtca
    28201 atgcgcacat tgttgccaac gtggacaatc aggttactgt taatcggaat cgacgccgtt
    28261 ttcggctctt ccggttgcag gttatcgtta agcatcacca catcgctgga gacgcctact
    28321 gcgctttccg gcagatcgtg caccacgatg cgcgcccacg ggacatccac gcgaccatcg
    28381 agggtaaaca ggtttggtgt agcctcgaag acaacatctg gcgatacatc cattcgtacc
    28441 atcggcggca cggtgatccg cactttactg cctttcgccg ttacccgcgc ccgccagttt
    28501 tcaatttggc tccagtcggc atcaccgttc aggtagattt caccctgctg ggtccgtact
    28561 gtaccggcaa gcgtcgagcg cataccatta aagttgaccg caagctggct cggttgcata
    28621 tcaaacggca taaagttgcc gtcgatatcc acacccgtaa cctgaagctg accaaacaac
    28681 tgcgggcttt gcacatcacc acccagacgc aagttggcac tcaccatccc cgctgctttt
    28741 tccccacggg taaagatggg gtttatcatc gcaaggttga agttacggat attgacgttg
    28801 ccaccaagat tacggcggcc ttgcggatcg gtcacctgca cctgtccatc aaactggccg
    28861 ttattggtca ggcggatggt ccagcccaat tcggcacggt tgttacgcaa ttccgccgtc
    28921 agattcagtg tctgaaacgc cacgggcagc gccgcatcgt tgacggtttg cgttacctgc
    28981 acgttacgcc ccgaaagggt gatactgccc tgcggcagcc cctctttggt ggtgtcccag
    29041 gcaacgtccg ctttacccgt gaagataccg ctggcctgag tggtttctgg cataaatggt
    29101 ttcagcatgg ccaggtcgaa gcggttgaga ttcaccaccg cacgcccttc ggcacccgca
    29161 tcgatagttt gcggcacgca cagttccgca ttcgggttaa gccagcagtg tggcccgatg
    29221 ctgatttttt gctccttatt gcggtaatcc agcgcaatat cgcgggtcag cgaccacggg
    29281 ccgaccggcg tctggaagcg ggtattgcta agagttccct tccagcgttc ttctttgcga
    29341 tcaaaacttc ctgccagatt aagctgcccg gagacaggct cgccctgaat ccgcaactgt
    29401 aactcgtgct gtttttcgct gcctttggca ttcagggtga cgaggttgat atttacatcc
    29461 ggctgcgaaa tttgctcaac gcgtacgtcg agtttcccgg cgatctgatc ggtggattta
    29521 atgtcgcctt ccacgcgaac ctgcgccacg gaaagttcct gccagcgcag gccgcgcgcg
    29581 gtgatatctg ccagtagttg tggcgcttcc accgtgccgc gtactttcac cagccctttc
    29641 gctgtaccgc caagccccgg cagcgcgtta tccagccccg gcgcgttgat ggtggcatca
    29701 agattgagat cttttacccc cagctcgcct ttcacttcgg cactgtttgg ccccagttcc
    29761 agatgaagcc ctgggatcat ccactgcata taactgttgc ctttcagcgt gccgtcaacg
    29821 ttcactttgt tctgtttgac gttaccggtc agcttcagtt ctggcacctc catctgccag
    29881 gtgccgccgt acaggctacc gcgggtttta atcaagccat tgagtttcga cggccactct
    29941 gggatctctt tggcggtgtt aatgccgtta agcgttagct caccgcgcca actaatggcc
    30001 tgctgccagt cgagcaacgc cttgagttca gttttccctt ccagcgccgc gacggtgagt
    30061 ttgtcgagat tgacctgctg ttcattacct ttggcatcaa gggtaatggt ggctggcggg
    30121 atctccagtc ccttcactgc cgtacgcata gagagcgtgt aatcggtcat tttgccggta
    30181 agtttcagtt tcagatcatc cgcctgatac tgcttctcac cagtgaacgg ccagtaaatc
    30241 tgtttgctgt tcacttccac gttgagcggc aatccggctt ccgccagtcg cgtctgggcg
    30301 cgtaaatcca tatccaccgg accggaaaga ttaacgccaa tctccagctg ttcgcgcagc
    30361 gcgccgccca ctttcagctt caccttttca cctttcaacg gctccacgtt cagtgtacta
    30421 ttgagagtga tgtccaccgg ccagttgtct gacagctgcg ccgtgccgct ggcgttgacg
    30481 atcccttgac tggaatcgat atccagggcg tccagtttag tattgccgtc aatgctgctc
    30541 actttcagca gcatggtgct cacggtgatg tccgtgtcgc ccgtcacgcg caactgctcg
    30601 cccttaaact cttcaatgtt caggttaagc ggcaaatgca cgtcggtcat ttccggcaat
    30661 accgggcgag aaaagagatc tttcagcgtt tcgccgagcg gcttttcctc cggctgcgga
    30721 ttttcaattt tcggttcgac cacttcttcc tgcgccactt ccgccacttt cggcagagca
    30781 atcagcaggc ctttcagcga cgtcggtttc agggtcaggg ttttctcctg ccagttcagg
    30841 ccggaggtga agtccatcac cgataccgtg gtgtcatcaa tcttgatgtt gacgttgtcc
    30901 agtgccaccc gtgttagggt gatgggatac ggcgtggaga gatccagcgg accgctatct
    30961 tcctcttctt caacctgttc agaagggggc atttttttgc tgtcgatatt gacctggatg
    31021 tctttcagcg ccagatcatt aatacaaaca ctgctgttcc acaggcactc aagcccgacc
    31081 gccagatgca gattgcctgc ttttaccgcc acgcctggct gctcataacg aacgtcagac
    31141 aaggtgagat cgcgccagcc gccggtgacc ttgccaacat ccagtcctgg cacccagcga
    31201 tccgccgctt taaataccag atgcaggccg ctggtggtgc ccaccagaaa cgccaccgat
    31261 cccagcaaca gtaagataac gataaccacg ccgaggctga tttttttcca taaactcata
    31321 attctggccc cagaccgatg taaaactgta acccgtgttc gtctttatcc gcgaccggta
    31381 cggcaaaatc gagtttgatt ggcccgaccg gcgattccca gcgtacgccg accccggtac
    31441 cggttttaaa gtcgctgcgg cgaatatcgc ttaccgcttc gccactatcg acaaacaccg
    31501 cgccccacca ttttccggtc acgttgtact ggtactccag cgatccggtt atcaacttcg
    31561 aggccccttt caggtcaccg ttggcgtatt tcggagcgat agatttgtat ttgtagccac
    31621 gaatactgcg gtcgcccccg gcgaaaaaac gcagatccgg cggtactttg tcgaaatcac
    31681 cggtttcaat ccagcccagc gtgccgcgtg tcacaaaacg atggcgatcg tacagtgtgc
    31741 ggatccagac gttttgtgcc tggaaaacgg agaaatcgac atctgagccc caggcagtgt
    31801 tggagtagtc gatagagtag cgttgcgagt cgccccaggt tggcatcagg ccaccacgag
    31861 aacgcgtgcg gctaatcatc accccaggat aaaacagcat cgtggtgttg gtaatttcac
    31921 cctgggtaaa gtggtcgaga ctccagcgca ggttaatggc acgctgccag ccgctggaga
    31981 gatcccagta gcgagaagcc accagcgtag tggagtcaga ttcggtatcg ttcaggtcag
    32041 tgcgcttaaa accgccctgc accaaataat attgttccag tggattcttc agcagcggca
    32101 ttttatagct gaagtcgagg atctgttccg gcgcggaaat actggtactg gtggtcagac
    32161 tgtgaccgta tgagttcatc cacggttttt tccacgtcgc tttcacgcgc ggtcccacgt
    32221 ccgtagagta accgaccccg gtttcgatgg tgttttctgt tcgcggcgaa accacgcccg
    32281 tcaatggtaa tactttcgtt tcgcgcgctt tatcaaattg tggagccacc accaccgagt
    32341 taaaccagcc ggtagcagaa agtcggcggt tcagttccgc cagatctttc gattcgtact
    32401 catcgccctc tttaaacggc accagatttt gcaggtattc atcgcggatt tgtgatcctt
    32461 caaaggtcac atgcccaaag cggtaacgtt cgccactgtt ataatcaata tcccagaagg
    32521 ctttatgcag gccgagcgca atgcccagct gcgctttggt aaattcgcta tcgaaataac
    32581 ctttacgcaa cgcaatgctg gttaaggact ttttgaaatt ttcataatcg ccctggttca
    32641 gcaccgtgcc aatagccggg cgagtatcga gcaatttcaa atagtcttta tcggtccgcg
    32701 cgccgccgcg caataccaca tcggtgccgc caattaacac cggcacgcct ggcgtgactt
    32761 tggcgatcaa tacctgccgc cctttctttg gcggtggacg gagatcaaat tcaatggtcg
    32821 gctggtaata acccagcgct ttcagacctt cgcggatggc atcatcgacg cgtgcgcgaa
    32881 agcgacggtc tggcgtcact tcatcacttt caatcgtaga aagctgcgca cgaacgttct
    32941 tttccagctg tcccgataac ccctcgacct gtagacggac gttcgcggcg acggcagatc
    33001 cgcttaagca gagtaagctt acacagcata actgtcggat atagcgcaca ttttctcctg
    33061 aatatccttt tttcctgccc ctggaacgcc gttaaacggc ttaacaaaaa tccagtaata
    33121 tggattaaaa agcagactaa accccaaata tttcttatgt tttactttag acctattcac
    33181 ggtggttatt gtgtgcaaat acgcctcttg ttacaacctt aaccccaatg accgattttc
    33241 gggagagcga caccatgagt ttatttgata aaaagcatct ggtttccccc gccgatgccc
    33301 tgcctggacg taacaccccg atgcccgtag ccacgctgca tgcggtcaac ggtcactcaa
    33361 tgaccaatgt acctgacgga atggagattg ccatttttgc gatgggttgt ttctggggtg
    33421 tggagcgtct gttctggcag ttacccggcg tttacagcac cgccgcaggc tataccggag
    33481 gctatacgcc aaatccgact tatcgggaag tgtgctccgg tgatacgggt catgccgaag
    33541 cggtacgcat tgtttacgat ccttccgtca tcagctatga gcagttgcta caggtatttt
    33601 gggagaatca cgatcccgcc cagggaatgc gtcagggcaa tgaccacggc acgcagtatc
    33661 gttcagcgat ttatccgctg accccagaac aggatgccgc agctcgcgcc agtctggaac
    33721 gttttcaggc ggcgatgctt gccgccgatg atgatcgtca catcaccacg gaaatcgcta
    33781 acgccacacc gttttattat gccgaagatg accaccagca atatctgcat aaaaacccgt
    33841 atggttactg tggaattggc ggaattggcg tctgtctgcc accggaagca tagcgttacg
    33901 ggtacaaatg tagattgttg ataaagtgcg ctttatttat gccagatgcg gcgtaaacgc
    33961 cttaccaggc ctacaaaatc gtccaaattc aatatattgc aaggactgcg taggcctgat
    34021 tggcatagcg catcaggcag tattgcattt atcagcagtc tgaattttaa ccctctggcg
    34081 actttacagt accttacgct atactagcca ctgaaaatgc cggttcactt tcttcgaatc
    34141 ggctttcaat gtgtatttca cacaaattaa tcaacttccc cttccgagga tctggcctga
    34201 aaggtcggat aagatatgtt aaacagtatt ttagtcatac tctgcttgat cgctgtaagt
    34261 gcgttcttct cgatgtccga gatctcactt gccgcctcac gcaaaatcaa acttaaactg
    34321 ctggctgatg aaggcaatat aaatgcccaa cgcgttctga atatgcagga aaatcccggc
    34381 atgttcttta ccgtggtcca aatcggtctg aacgcagtgg cgattctcgg cggtatcgtc
    34441 ggtgatgcgg cattttctcc agcttttcac agcctgttct cccgctatat gtcggcagag
    34501 ctctctgagc aactgagctt tattctctct ttctcgttag tgactggcat gtttatcctg
    34561 tttgcggatt taaccccgaa acgcatcggt atgattgcgc cagaagctgt cgctttgcgt
    34621 atcatcaacc cgatgcgctt ctgcctgtac gtttgcaccc cgctggtgtg gttcttcaac
    34681 ggactggcga acataatctt ccgtattttc aaactgccaa tggtacgtaa agatgacatc
    34741 acttctgatg acatctacgc ggtagtggaa gccggtgcgc tggcgggcgt gttacgtaaa
    34801 caggaacacg agctgattga aaacgtcttt gagctggaat cccgtaccgt tccgtcgtca
    34861 atgacaccgc gtgaaaacgt gatttggttt gatctccacg aagatgagca aagcctgaag
    34921 aataaggtgg cggaacatcc gcactctaag ttcctcgtct gtaatgaaga tattgaccac
    34981 atcatcggct atgtcgattc taaagacctg ctgaaccgcg tgctggctaa ccaaagcctg
    35041 gcactgaaca gcggcgtaca aattcgcaac acgctgattg tgccggatac gttaaccctt
    35101 tcagaggcgt tggaaagttt taaaaccgca ggtgaagact tcgcggtgat catgaacgag
    35161 tacgcgctgg tggtggggat catcaccctc aatgacgtga tgaccacgct gatgggcgat
    35221 ctagtcggtc aggggctgga agagcagatt gtcgcccgtg atgagaactc atggctgatt
    35281 gacggcggca ccccaattga cgacgtcatg cgcgtgctgg atattgacga gttcccgcag
    35341 tcgggcaact acgaaaccat cggcggcttt atgatgttta tgctgcgtaa gatcccgaaa
    35401 cgcaccgatt cggtgaaatt cgccggctac aaatttgaag tggtggatat cgataactac
    35461 cgcatcgacc agctgctggt gacccggatc gacagcaagg ccaccgccct ttcgccaaaa
    35521 ctgcctgacg ctaaagataa agaagaaagc gtcgcgtaac ccccagaaac atcaacggct
    35581 cctgaatcag gagccgtctt attactgcat agcactttgg ttaagccatc tctgtttgca
    35641 gacgcataac ctgacggtta acttcggaca tcactgacaa atgcagctta tccttcactt
    35701 ttgggataag aatcttaccc ttatcaaatt caaaagcgcc aacgtccttg atgtataacc
    35761 gtccacggaa caggatcttt acgtacttcg ccacctgaag tgggttgtac cgttggaaaa
    35821 ttttcattgt tatctcctgc tgagtattac gcctgtgcgg tgccacaatc ggcccaacta
    35881 ttatgaggcg caaattttaa tgcttagtga ctatagacta tccgggcaat gtttccaccg
    35941 cgtataactt tttttaccta aaggttacaa ttattcagaa ttatcttttt accgaagcgc
    36001 ggtcttcagt ataagcattc atttttcata tgatttgtgc gcttgaccgc aaactggcat
    36061 cacacttgcg ggaaattcga taaatagcac atatgattaa aactcagacc caagtggtcg
    36121 gatcacctgc atatcataag aaggaaacac catgacccta cgcaagattc tggcactcac
    36181 ctgcctgctg ttgccgatga tggcttccgc acatcagttc gaaaccggtc agcgagtgcc
    36241 gccgattggc attaccgatc gaggcgagtt ggtgcttgat aaagatcagt ttagctacaa
    36301 aacctggaac agcgcacagt tagtgggaaa agtgcgagta ctgcaacata ttgctggtcg
    36361 cacctctgca aaagagaaaa acgcgacgct gattgaagcg attaaatcag cgaagttacc
    36421 gcacgatcgc taccagacca ccaccattgt taacaccgac gacgcgattc cgggttcagg
    36481 gatgtttgtg cgcagcagtc tggagagtaa taaaaagctt tatccctggt cgcagtttat
    36541 tgtcgatagc aatggcgtcg cgcgcggtgc ctggcagctg gatgaagaaa gttccgctgt
    36601 agtggtgctg gataaggatg gtcgcgtgca atgggccaaa gacggggcgc tcacacagga
    36661 agaggtgcag caagtgatgg acctgctgca taaattaatt aataaataga cactctgaat
    36721 cccggattca ggaacgactc gcgcggagtg taatccagcg gtttaccctg ccagtcatga
    36781 acgtgcgctc cggcagctgc agccacagca tgaccagcgg cggtatccca aatattcgtt
    36841 ggtccgaagc gcgggtacag ctgcgcctgc ccttccgcca ccaggcagaa tttcagcgaa
    36901 gagccgatgg acgtggtctg atgttcgcca agctgttgca gatactcttt cagctccgca
    36961 tctgcatggg aacggctgat aaccaccagc ggcgggcgcg catcgcggac ctgaatctgc
    37021 ttgcgcacac cgcactcttc tttccaggcc ttgccttctg ccgcgctgta cattatgttc
    37081 attaccggcg catacaccac acctaaaatc ggtttgccat ggtcaatgag cgcaatgtta
    37141 acggtgaatt cgccattacg tttaataaac tctttagtac catccagcgg gtctaccagc
    37201 cagtaacgct gccagtgctg acggacttcc caaccgggag gatcttcttc agaaaggacc
    37261 ggaatatccg gtgtcagcgt acgtaaaccg tccatgataa cggtgtgagc ggcaatatcc
    37321 gctgccgtta ccggagaatt gtccgctttg ctgacgacgt ccatcggttt cgtcccgtcg
    37381 tagacctgca taatggcatc gcctgcattc cgtgcaagct ggcatacttg atctaacatt
    37441 tctccacctc gtctctgtga gcggtgttaa cttattgttt tacttatacc ctatcgttaa
    37501 tgaatgcgcc aactgtgata gtgtcatcat tttcaaagcg taaaattgtg gcattcttca
    37561 ctgttctata agtaaggcgt ttattctccc ttttctttcg tattcccgat gataaaagga
    37621 tgtccctgat gattaagttt agcgcaacgc tcctggccac gctgattgcc gccagtgtga
    37681 atgcagcgac ggtcgatcta cgtatcatgg aaaccactga tctgcatagc aacatgatgg
    37741 atttcgatta ttacaaagac accgccacgg aaaaattcgg actggtacgt acggcaagcc
    37801 tgattaacga tgcccgcaat gaagtgaaaa acagcgtact ggtcgataac ggcgatttga
    37861 ttcaggggag tccgctggcc gattacatat cggcgaaagg attaaaagca ggtgatgttc
    37921 atccggttta taaggcgctg aatacgctgg attatacggt cggtacactc ggcaatcatg
    37981 aatttaacta cggtctggat tacctgaaaa atgcgttggc gggagcgaaa ttcccttatg
    38041 taaatgccaa cgtcattgac gccagaacca aacagccaat gtttacaccg tatttaatta
    38101 aagataccga agtggtcaat aaagacggaa aaaaacagac gctgaagatt ggctatattg
    38161 gcgtcgtacc gccgcaaatc atgggctggg ataaagctaa tttatccgga aaagtgacgg
    38221 tgaatgatat taccgaaacc gtgcgcaaat acgtgcctga aatgcgcgag aaaggtgccg
    38281 atgtcgttgt cgttctggcg cattccgggc tatctgccga tccgtataaa gtgatggcgg
    38341 aaaactcagt ttattacctc agtgaaattc ctggcgttaa cgccattatg tttggccatg
    38401 ctcacgccgt tttcccaggt aaagattttg ctgatatcga aggggctgat atcgcgaaag
    38461 gcacgctgaa tggtgttccg gcggtaatgc cgggcatgtg gggcgatcat cttggggtgg
    38521 tcgacttaca actcagtaat aacagcggta aatggcaggt gacgcaggcg aaagcggaag
    38581 cacggccgat ttacgacatc gccaataaaa aatccctcgc ggcggaagac agcaagctgg
    38641 tagaaacact caaagccgat cacgatgcca cacgccagtt cgtcagcaag ccaatcggta
    38701 aatccgccga caatatgtat agctatctgg cgctggtgca ggacgatccg accgtgcagg
    38761 tggtgaacaa cgcgcaaaaa gcgtatgtcg agcattacat tcagggcgat ccggatctgg
    38821 caaaactgcc ggtgctttca gctgctgcac cgtttaaagt tggtggtcgc aaaaatgatc
    38881 cggcaagcta tgtggaggtg gaaaaaggcc agttgacctt ccgtaatgcc gccgatcttt
    38941 atctctaccc caatacgctg attgtggtga aagccagcgg taaagaggtg aaagagtggc
    39001 tggagtgctc cgccggacag tttaaccaga ttgatcctga caacacgaaa ccacagtcac
    39061 tcatcaactg ggatggtttc cgcacctata actttgatgt gattgatggt gtgaattatc
    39121 agattgatgt tacccagcct gcccgttatg acggcgagtg ccagatggtt aatgccaatg
    39181 cggaaaggat taagaacctg acctttaatg gcaagccgat tgatccgaac gccatgttcc
    39241 tggttgccac caataactat cgcgcttacg gcggcaaatt tgccggtacg ggcgacagcc
    39301 atatcgcttt tgcttcaccg gatgagaacc gctcggtgct ggcagcgtgg attgctgatg
    39361 agtcgaaacg tgcgggggaa attcacccgg cggcagataa caactggcgt ttagcaccga
    39421 tagctggcga taagaaactg gatatccgtt tcgaaacctc tccatcagat aaagccgcgg
    39481 cgtttattaa agagaaaggg caatatccga tgaataaagt cgcgaccgat gatatcgggt
    39541 ttgcgattta tcaggtggat ttgagtaagt aatacacttc tttttcggcc tataaatcat
    39601 caaccgcatc cggcactttt gtcggatgcg atgctggcgc atcttatccg ccctacaagt
    39661 catgaaccgt aggccggata agcgcagccg gcagttatgc cgcacgttca tcccgcaccg
    39721 ccaacacctc gggcaaattc aactcaatcc agtccgccag tgcagcaacc ttttcgctca
    39781 cctgctcgcc cagcggcgtg aggctatatt ccacatgcgg cggcaccacc ggatacgcga
    39841 tacggttaag aaaaccatcc tgttctaacg cctgtaacga ctgcgcaagc atcttttcac
    39901 tcaccccacc gattttgcgc cgcaggtcgc taaagcgatg agtaccttcg cgtagcgcca
    39961 ccagaatcaa caccccccaa cggctggtga cgtgtttcaa cacctcgcgc gacgggcact
    40021 gttccgcaaa gagattaccc tctttcagtt gttgtgacag gctaacctga ctcatttcat
    40081 acttaccttt ttgtacgtac ttactaaaag taagtttagg tgttagcgta tttaaacaca
    40141 agacaaaacg atggagactt cccatgatcg ctattactgg tgccactggc caacttggtc
    40201 actatgttat taaatccttg atgaaaacgg ttcctgccag ccaaatagtg gctatcgttc
    40261 gtaatccggc aaaagcccag gcactgacag cacaaggcat taccgtgcgt caggctgact
    40321 acggcgatga agccgcactg acatctgcac ttcagggagt ggaaaaacta ctgctgatct
    40381 cttccagcga agtgggtcaa cgtgccccgc agcatcgtaa tgttattaat gccgcaaaga
    40441 cggctggcgt gaaatttatc gcttatacca gtctgcttca cgcggataaa tctccgctcg
    40501 gcctcgccga tgagcacatc gagacggaga aaatgttggc tgattctggc atcgtttaca
    40561 ccctgctgcg caacggctgg tacaccgaaa actatctcgc cagcgccccg gcagcactgg
    40621 agcacggcgt atttatcggt gccgcgggcg atggcaaaat tgcctcggca acgcgggcag
    40681 attatgcggc agctgcggca cgcgtgatta gcgaagccgg tcacgaaggc aaggtttacg
    40741 agctggcggg cgatagtgcc tggacgttga cacagttagc ggcagagctg accaaacaga
    40801 gcggcaaaca ggttacctat caaaatctga gcgaaaccga tttcgctgcg gcgctgaaaa
    40861 gcgttggcct gcctgacgga ctggcggata tgctggcgga ttctgacgtt ggcgcatcga
    40921 aaggcggtct gtttgatgac agcaaaacgc tgagcaaatt gattggccgc ccaacgacaa
    40981 cgttagccga aagcgtaagc catcttttta atgttaataa atagttaatt aaagtggcat
    41041 cctcccgcat cctctctgat aatgacggga tgccgggagc aatcatgtct gcttcctgaa
    41101 cgtttcttct gacagaccaa tggatgccag taatgattag cggcgtgctg tacgccctgt
    41161 tagcagggtt gatgtggggg cttatttttg tcgggccgtt gatcgtgccg gaatacccgg
    41221 cgatgttgca gtcgatgggg cgttatctgg cgttagggtt aattgcgcta cccattgcct
    41281 ggctgggacg cgtgcgtctg cgtcagttgg cgcgtcgcga ctggcttacc gccttgatgc
    41341 tcactatgat gggcaacctc atctattact tctgccttgc cagtgccatt caacgtactg
    41401 gcgcgcctgt ttctacgatg attattggca ccctgccggt ggtcattcct gtctttgcca
    41461 atctgcttta tagccagcgc gacggcaaac tcgcgtgggg aaaactcgcc ccggcactga
    41521 tttgtattgg catcggcctg gcgtgtgtga atattgctga gttaaaccac ggactccccg
    41581 attttgactg ggcacgttat acctctggca tcgtgctagc gttagtttcc gtggtctgct
    41641 gggcatggta tgccctgcgc aacgcccgct ggctgcggga aaatcccgac aaacatccga
    41701 tgatgtgggc gacggcgcag gcgctggtca cgctgccggt ttctctcatc ggctatctcg
    41761 tcgcctgtta ctggctgaat atacaaacgc cggacttctc cttacctttt ggcccccgtc
    41821 cgctggtgtt tattagtctg atggttgcga tagccgtgct ttgctcatgg gttggcgcac
    41881 tctgctggaa cgtcgccagc cagcgattac cgacagtgat tctcgggccg ctgatcgttt
    41941 tcgaaacact ggcaggtttg ctgtacacct ttttgatacg ccagcaaatg ccgccgctga
    42001 tgacgctgag cggtatcgcg ctgttagtgg ttggcgtggt cattgcagtc agagcaaaac
    42061 cggaaaagcc tttaactgaa tctgtctcag aaagttgaca tactggcagt gagttaaata
    42121 agcctctgct acgtaagggt tatagcattt gccttaaaga tgcatttaaa atacatctta
    42181 tcttattaag aatgaggtat cagctatggc ttatcgcgac caacctttag gtgaactggc
    42241 gctctctatc cctcgcgctt cggctctgtt tcgtaaatat gatatggatt actgctgcgg
    42301 cggtaagcaa acgctggcgc gtgcggcggc acgtaaagaa ctggatgttg aggtcattga
    42361 agctgagctg gcaaagcttg ctgaacaacc gattgagaaa gactggcgta gcgccccgct
    42421 ggcagaaatc atcgaccata tcatcgtgcg ctaccacgat cgtcaccgcg agcaactgcc
    42481 ggagctgatc ctgcaagcga ctaaagtcga gcgcgttcac gccgacaaac cgagcgtgcc
    42541 aaaagggctg acaaaatacc tgaccatgct gcatgaagag ctttccagcc acatgatgaa
    42601 agaagagcag atcctcttcc cgatgatcaa acaaggcatg ggcagccagg caatggggcc
    42661 aatcagcgta atggaaagcg agcacgatga agcgggcgaa ctgctggaag tgatcaaaca
    42721 cactaccaat aacgtcacac cgccgccaga agcctgcacc acctggaaag cgatgtataa
    42781 cggcattaat gaactgattg atgacctgat ggatcacatc agtctggaaa acaatgtact
    42841 gttcccacgc gcgctggcgg gtgagtaatc taaaagctgg atggcattgc gccatccagc
    42901 atgataatgc ggttatttcc gcagttcagc agcccgcttc ttaccaataa acaaccagcc
    42961 cagccccagt gcgataaacc acagcggggt gaccaacagc gcctggcgag tgtcatcttc
    43021 cagtgtcagc aacacgacca cgaacacaaa gaacgccata catacccagc acatcagctt
    43081 gccgagcggc atcttgtaga tcgacttctc atgcagatga ggacgctgtt tgcggtaaac
    43141 caggtacgag caaaggataa tcgtccagac gaacataaac agaatcgcgg aaacggttgt
    43201 aatcatcgtg aacgcgccaa tcacgctagg attcacatac agcatcacca cgccaccgag
    43261 caggcagatg caggagaagg tcagcccttt cgctggtact gcgcgcttag aaagtttagc
    43321 gaacgctttc ggtgccacac cttcctgcgc cagaccaaac agcatacggc tggtagagaa
    43381 gacgccgctg ttagcggaag acgccgcaga ggtcagcacc acaaagttga tcacgcttgc
    43441 ggcagcaggc agccctacca acacgaacag ttcaacaaac gggcttttct ccgggactac
    43501 cgaactccac ggcgtcacgg acataatcac aatcagcgcg aagacgtaga acataatgat
    43561 acggatcgga atggagttaa tcgcgcgtgg cagtgatttc tccggatctt tggtttccgc
    43621 agctgttgta cctaccagct caatccccac gaaagcgaaa accgctatct ggaatccggc
    43681 aaagaagcca cttaaacctt tcgggaacca gccgccgtca ttccacaaat gcgcgaatga
    43741 cgcttccaca ccggtcggtg actgaaagtg catcgccacc atgaccaggc cgacgacaat
    43801 cagcgacacg atggcgacga ttttgatcat cgcaaaccag aactccatct caccgaacat
    43861 tttcacggtg gcaaggttga gcgtcaacag cagcactatc accgccagcg aggcgaccca
    43921 gtcggagaga tcggggaacc agaactgagc ataagcagtg atcgccacca cgtctgccat
    43981 accggttaca acccagcaga accagtaagt ccagccggtg aaatatcctg cccacggccc
    44041 gagtaaatcg gaagcgaagt cactaaaaga tttgtattcc agattcgaaa gcagcaattc
    44101 ccccattgcc cgcatcacga aaaagagcat aaaaccgatg atcatataaa cgaaaatgat
    44161 cgacggcccg gcaaggctaa tcgttttgcc agaccccata aacaaccccg taccaatggc
    44221 accgccaatg gcaataagct gaatatgtcg gtttgtgaga ttgcgccgta gcgactgttc
    44281 agccggagcc tgatcatcgg caacgacttt tacctgatct accatgtttt tttcttcctg
    44341 tacctgtctg tgttgttcag gctctacggc ctttagtcag tctatgatac gacgaatctg
    44401 tatcgggact catcgatatt aggtaacaat acgcgggatg aatactaaga tttagatcta
    44461 atgttaattt tatgtttaaa gtgagtgtca tatcacggtg ataacgcgaa acagctcaca
    44521 aaaatacacg cttacggggt ctttgcaaca caaaatagcg ccgtttggca aactgaaggg
    44581 tttattgctg aatgcctgct cccctctggt tcgaggggaa cagaatgcgc tgcttagagg
    44641 atttccagca gttcgacttc aaacaccagg gtgctgaacg gagggatgga tgcgcctgcg
    44701 ccgcgctcgc catatgccag ttcctgcggg atagtcagtt cccatttaga acctaccggc
    44761 atcagagtca gtgcttcaat ccagccaggg atcacgccat taaccgggaa ttcagcgggt
    44821 tcaccacgag caacggagct gtcaaacacg gtgccgtcga tcagtttacc ggtgtaatga
    44881 acacgaacgc ggtcagtacg tgccggaatt gcgccttcac cctggttgat cacgcggaat
    44941 tgcaggccag attcggtgct gttcacacct tcttttttgg cattttcttc aagatatttc
    45001 acaccttcgg cagccatcgc ctggaaacgc tgacgacgaa cggcatcggc gcgttcgtgg
    45061 atttcacgca gcgcgcgatg caccacatca accggaacag ccggatgttt gccttccagc
    45121 gcatctgcaa taccagccac cagcgcctcc ggcagcagcc cttgcaggcc agattcactc
    45181 agttgttggc ctacctgtaa accaatgccg tagcttgctt gcgcttcgat ggtgtcaaaa
    45241 gttggggtgg tcatcgtatt tcctttctta gggtataaaa gtagcgggca gcatatcagc
    45301 cacacttctt tgggtaaaac tttgtcgcag gaatgatgac aaatcccggg gaaaaagaaa
    45361 caatagcaat atcccgtatg aatataataa aggcgggttt accacggaca tcatagcgtt
    45421 agcttattca taggctatga ttgaggaaca agacgcggag caggaggaaa ccatgcccgg
    45481 gcgctttgaa ctaaaaccaa ccctggagaa agtctggcac gcgccggaca attttcgctt
    45541 catggacccg ctgccgccga tgcatcgtcg ggggatcatt attgccgcca ttatattggt
    45601 ggtcggattt ctgcttccat ctgatgatac gcccaacgcg ccggtggtga cgcgtgaagc
    45661 gcagttagat attcagtcgc aatcacaacc gccgacagaa gaacagctgc gggcgcaatt
    45721 agtcacacca caaaatgatc ctgatcaggt ggctccggtc gctccagaac ctatacagga
    45781 aggccagcca gaagaacagc cgcaaactac ccaaacacaa ccattccagc cagatagcgg
    45841 aattgataat caatggcgct cttaccgtgt cgaacccggt aaaacgatgg cgcagctgtt
    45901 ccgcgaccac ggactgcccg ctaccgatgt ctatgcaatg gcgcaggtag aaggcgcggg
    45961 taagccgctg agtaatttgc aaaacgggca aatggtgaaa atccgccaga acgccagcgg
    46021 cgtggtgacg ggcttaacca ttgatacggg gaataatcaa caggtcctgt tcacccgcca
    46081 gccggatggc agttttattc gtgcgcggta gatgaagggt caaccaaaag ccagctaagg
    46141 attgtctctt tgatgacctt acgtctggct tccagtgcct ggggggaaac catactgata
    46201 tgataaacca ggccaagcgt atgctggttg atcaaataat atccgccaag cgcagcaata
    46261 ttaatattca cctgcaatgg gtcgatatct ggcttaaaaa tattgtgttt cttcccttca
    46321 tccaataagg attccattaa ctgcaagtgc gcatggttaa tttctaagag acgctgtgat
    46381 tttgcataat gcacaccttt actttgattt tcactatgca caatttttaa aaaccag
//
LOCUS       DAPHBJ010000012        43290 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.012, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000012 DAPHBJ010000000
VERSION     DAPHBJ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.012"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            238..1185
                     /gene="cyoA"
                     /locus_tag="RHZ76_000649"
     CDS             238..1185
                     /gene="cyoA"
                     /locus_tag="RHZ76_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HDV2811342.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            1207..3198
                     /gene="cyoB"
                     /locus_tag="RHZ76_000650"
     CDS             1207..3198
                     /gene="cyoB"
                     /locus_tag="RHZ76_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HDV2811343.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            3188..3802
                     /locus_tag="RHZ76_000651"
     CDS             3188..3802
                     /locus_tag="RHZ76_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HDV2811344.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPERSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3802..4131
                     /locus_tag="RHZ76_000652"
     CDS             3802..4131
                     /locus_tag="RHZ76_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HDV2811345.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            4143..5033
                     /gene="cyoE"
                     /locus_tag="RHZ76_000653"
     CDS             4143..5033
                     /gene="cyoE"
                     /locus_tag="RHZ76_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HDV2811346.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            5182..6546
                     /locus_tag="RHZ76_000654"
     CDS             5182..6546
                     /locus_tag="RHZ76_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2811347.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVIAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWINGMFDGQGVFLAGAMLAAVWLTVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEIEQA
                     IRQA"
     gene            complement(6674..7165)
                     /gene="yajQ"
                     /locus_tag="RHZ76_000655"
     CDS             complement(6674..7165)
                     /gene="yajQ"
                     /locus_tag="RHZ76_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HDV2811348.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            7333..8244
                     /gene="panE"
                     /locus_tag="RHZ76_000656"
     CDS             7333..8244
                     /gene="panE"
                     /locus_tag="RHZ76_000656"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDV2811349.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            8207..8797
                     /gene="yajL"
                     /locus_tag="RHZ76_000657"
     CDS             8207..8797
                     /gene="yajL"
                     /locus_tag="RHZ76_000657"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HDV2811350.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRSGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWLDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(8851..10299)
                     /gene="thiI"
                     /locus_tag="RHZ76_000658"
     CDS             complement(8851..10299)
                     /gene="thiI"
                     /locus_tag="RHZ76_000658"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDV2811351.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            10505..10747
                     /gene="xseB"
                     /locus_tag="RHZ76_000659"
     CDS             10505..10747
                     /gene="xseB"
                     /locus_tag="RHZ76_000659"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDV2811352.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            10747..11646
                     /gene="ispA"
                     /locus_tag="RHZ76_000660"
     CDS             10747..11646
                     /gene="ispA"
                     /locus_tag="RHZ76_000660"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HDV2811353.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            11671..13533
                     /gene="dxs"
                     /locus_tag="RHZ76_000661"
     CDS             11671..13533
                     /gene="dxs"
                     /locus_tag="RHZ76_000661"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDV2811354.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            13713..14687
                     /gene="yajO"
                     /locus_tag="RHZ76_000662"
     CDS             13713..14687
                     /gene="yajO"
                     /locus_tag="RHZ76_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HDV2811355.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(14741..15259)
                     /gene="pgpA"
                     /locus_tag="RHZ76_000663"
     CDS             complement(14741..15259)
                     /gene="pgpA"
                     /locus_tag="RHZ76_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDV2811356.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(15237..16214)
                     /gene="thiL"
                     /locus_tag="RHZ76_000664"
     CDS             complement(15237..16214)
                     /gene="thiL"
                     /locus_tag="RHZ76_000664"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDV2811357.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(16292..16711)
                     /gene="nusB"
                     /locus_tag="RHZ76_000665"
     CDS             complement(16292..16711)
                     /gene="nusB"
                     /locus_tag="RHZ76_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDV2811358.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(16731..17201)
                     /gene="ribE"
                     /locus_tag="RHZ76_000666"
     CDS             complement(16731..17201)
                     /gene="ribE"
                     /locus_tag="RHZ76_000666"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDV2811359.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(17290..18393)
                     /gene="ribD"
                     /locus_tag="RHZ76_000667"
     CDS             complement(17290..18393)
                     /gene="ribD"
                     /locus_tag="RHZ76_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDV2811360.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQLLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPIRIVIDSQNRVTPVHRIVQQPGETWFARTQEDSREWPETVRTLLIPAHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(18397..18846)
                     /gene="nrdR"
                     /locus_tag="RHZ76_000668"
     CDS             complement(18397..18846)
                     /gene="nrdR"
                     /locus_tag="RHZ76_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HDV2811361.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            18997..19536
                     /locus_tag="RHZ76_000669"
     CDS             18997..19536
                     /locus_tag="RHZ76_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001430049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDV2811362.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMH"
     gene            19835..20719
                     /gene="tsx"
                     /locus_tag="RHZ76_000670"
     CDS             19835..20719
                     /gene="tsx"
                     /locus_tag="RHZ76_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HDV2811363.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(20896..21243)
                     /locus_tag="RHZ76_000671"
     CDS             complement(20896..21243)
                     /locus_tag="RHZ76_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HDV2811364.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(21371..22342)
                     /gene="secF"
                     /locus_tag="RHZ76_000672"
     CDS             complement(21371..22342)
                     /gene="secF"
                     /locus_tag="RHZ76_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HDV2811365.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(22353..24200)
                     /gene="secD"
                     /locus_tag="RHZ76_000673"
     CDS             complement(22353..24200)
                     /gene="secD"
                     /locus_tag="RHZ76_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HDV2811366.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(24228..24560)
                     /gene="yajC"
                     /locus_tag="RHZ76_000674"
     CDS             complement(24228..24560)
                     /gene="yajC"
                     /locus_tag="RHZ76_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDV2811367.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(24583..25710)
                     /gene="tgt"
                     /locus_tag="RHZ76_000675"
     CDS             complement(24583..25710)
                     /gene="tgt"
                     /locus_tag="RHZ76_000675"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDV2811368.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(25766..26836)
                     /gene="queA"
                     /locus_tag="RHZ76_000676"
     CDS             complement(25766..26836)
                     /gene="queA"
                     /locus_tag="RHZ76_000676"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDV2811369.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            26929..27510
                     /gene="acpH"
                     /locus_tag="RHZ76_000677"
     CDS             26929..27510
                     /gene="acpH"
                     /locus_tag="RHZ76_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HDV2811370.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASRKAL"
     gene            complement(27515..29332)
                     /gene="malZ"
                     /locus_tag="RHZ76_000678"
     CDS             complement(27515..29332)
                     /gene="malZ"
                     /locus_tag="RHZ76_000678"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HDV2811371.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(29488..30861)
                     /gene="proY"
                     /locus_tag="RHZ76_000679"
     CDS             complement(29488..30861)
                     /gene="proY"
                     /locus_tag="RHZ76_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HDV2811372.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(30937..32256)
                     /gene="brnQ"
                     /locus_tag="RHZ76_000680"
     CDS             complement(30937..32256)
                     /gene="brnQ"
                     /locus_tag="RHZ76_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HDV2811373.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIIWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(32663..33958)
                     /gene="phoR"
                     /locus_tag="RHZ76_000681"
     CDS             complement(32663..33958)
                     /gene="phoR"
                     /locus_tag="RHZ76_000681"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HDV2811374.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(34016..34705)
                     /gene="phoB"
                     /locus_tag="RHZ76_000682"
     CDS             complement(34016..34705)
                     /gene="phoB"
                     /locus_tag="RHZ76_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HDV2811375.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            34895..36097
                     /gene="sbcD"
                     /locus_tag="RHZ76_000683"
     CDS             34895..36097
                     /gene="sbcD"
                     /locus_tag="RHZ76_000683"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HDV2811376.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            36094..39237
                     /gene="sbcC"
                     /locus_tag="RHZ76_000684"
     CDS             36094..39237
                     /gene="sbcC"
                     /locus_tag="RHZ76_000684"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HDV2811377.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQHLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            39363..40547
                     /gene="araJ"
                     /locus_tag="RHZ76_000685"
     CDS             39363..40547
                     /gene="araJ"
                     /locus_tag="RHZ76_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HDV2811378.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADTPVLAKP
                     LG"
     gene            complement(40792..41700)
                     /gene="mak"
                     /locus_tag="RHZ76_000686"
     CDS             complement(40792..41700)
                     /gene="mak"
                     /locus_tag="RHZ76_000686"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HDV2811379.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFAMDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            41825..42736
                     /gene="rdgC"
                     /locus_tag="RHZ76_000687"
     CDS             41825..42736
                     /gene="rdgC"
                     /locus_tag="RHZ76_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDV2811380.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(42814..42897)
                     /locus_tag="RHZ76_000688"
     CDS             complement(42814..42897)
                     /locus_tag="RHZ76_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2811381.1"
                     /translation="MTQRSWSKLQRKTHNIAALKIIARRSE"
     gene            complement(42894..43235)
                     /locus_tag="RHZ76_000689"
                     /pseudo
     CDS             complement(42894..43235)
                     /locus_tag="RHZ76_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT        10532 a        11559 c        11077 g        10122 t
ORIGIN      
        1 tttatttcag attggttata ccaattgccc gtccaaactc cttctgcact cccctttcgt
       61 tataacgccc ttttgcaaca gcttcttaaa atcaacttga tatgttttgc aacatatgtg
      121 acctgacagc caaatccaag taacaggaat ttaatcatgt ttacagtaat gtaaccttcc
      181 cgtaaaatgc ccacacactt taaacgccac cagatcccgt ggaattgagg tcgttaaatg
      241 agactcagga aatacaataa aagtttggga tggttgtcat tatttgcagg cactgtattg
      301 ctcagtggct gtaattctgc gctgttagat cccaaaggac agattggtct ggagcaacgt
      361 tcactgatac tgacggcatt tggcctgatg ttgattgtcg ttattcccgc aatcttgatg
      421 gctgttggtt tcgcctggaa gtatcgtgcg agcaataaag atgctaagta cagcccgaac
      481 tggtcacact ccaataaagt ggaagctgtg gtctggacgg tacctatctt aatcatcatc
      541 ttccttgcgg tactgacctg gaaaaccact cacgctcttg agcctagcaa gccgctggca
      601 cacgacgaga agcccattac catcgaagtg gtttccatgg actggaaatg gttcttcatc
      661 tacccggaac agggcattgc taccgtgaat gaaatcgctt tcccggcgaa cactccggtg
      721 tacttcaaag tgacctccaa ctccgtgatg aactccttct tcattccgcg tctgggtagc
      781 cagatttatg ccatggccgg tatgcagact cgcctgcatc tgatcgccaa cgaacccggt
      841 acttatgacg gtatctccgc cagctacagc gggccgggct tctcaggcat gaagttcaaa
      901 gctattgcaa caccggatcg cgccgaattc gaccaatggg tcgcaaaagc gaaacagtcg
      961 ccgaacacca tgtctgacat ggctgcgttc gaaaaactgg ccgcgcctag cgaatacaac
     1021 caggtggaat atttctccaa cgtgaaacca gacttgtttg ccgatgtgat taacaagttt
     1081 atggctcacg gtaagagcat ggacatgacc cagccagaag gtgagcacag tgcacacgaa
     1141 ggtatggaag gcatggacat gagccacgcg gaatccgccc attaaagggg ttgaggaaga
     1201 ataaagatgt tcggaaaatt atcacttgat gcagtcccgt tccatgaacc tatcgtcatg
     1261 gttacgatcg ctggcattat tttgggaggt ctggcgctcg ttggcctgat cacttacttc
     1321 ggtaagtgga cctacctgtg gaaagagtgg ctgacctccg tcgaccataa acgcctcggt
     1381 atcatgtata tcatcgtggc gattgtgatg ttgctgcgtg gttttgctga cgccattatg
     1441 atgcgtagcc agcaggctct tgcctcggcg ggcgaagcgg gcttcctgcc acctcaccac
     1501 tacgatcaga tcttcaccgc gcacggcgtg attatgatct tcttcgtggc gatgcctttc
     1561 gttatcggtc tgatgaacct ggtggttccg ctgcagatcg gcgcgcgtga cgttgcgttc
     1621 ccgttcctca acaacttaag cttctggttt actgttgttg gtgtgattct ggttaacgtt
     1681 tctctcggcg tgggcgaatt tgcgcagacc ggctggctgg cctatccacc gctatcggga
     1741 atagagtaca gtccgggagt cggtgtcgat tactggatat ggagtctcca gctatccggt
     1801 ataggtacga cgcttaccgg tatcaacttc ttcgttacca ttctgaagat gcgcgcaccg
     1861 ggcatgacca tgttcaagat gccagtattt acctgggcat cactgtgcgc gaacgtactg
     1921 attattgctt ccttcccaat tctgacggtt accgtcgcgt tgttgaccct ggatcgctat
     1981 ctgggcaccc atttctttac caacgatatg ggtggcaaca tgatgatgta catcaacctg
     2041 atttgggcct ggggccaccc ggaagtttac atcctgatcc tgcctgtttt cggtgtgttc
     2101 tccgaaattg cggcaacctt ctcgcgtaaa cgtctgtttg gttatacctc gctggtatgg
     2161 gcaaccgtct gtatcaccgt gctgtcgttc atcgtttggc tgcaccactt ctttacgatg
     2221 ggtgcgggcg cgaacgtaaa cgccttcttt ggtatcacca caatgattat cgccatcccg
     2281 accggggtga agatcttcaa ctggctgttc accatgtatc agggccgcat cgtgttccat
     2341 tctgcgatgc tgtggaccat cggttttatc gtcactttct cggtgggcgg gatgactggc
     2401 gtgctgctgg cagtaccggg cgcggacttc gttctgcata acagcctgtt cctgattgcg
     2461 cacttccata acgtgatcat cggcggcgtg gtcttcggct gcttcgcagg gatgacctac
     2521 tggtggccta aagcgttcgg tttcaaactg aacgaaacct ggggtaaacg cgcgttctgg
     2581 ttctggatca tcggcttctt cgttgccttt atgccgctgt atgcgctggg cttcatgggc
     2641 atgacccgtc gtttgagcca gcagattgac ccgcagttcc acaccatgct gatgattgca
     2701 gccagcggtg cagtactgat tgcgctgggt attctctgcc tcgttattca gatgtacgtt
     2761 tctattcgcg accgcgacca gaaccgtgac ctgactggcg acccgtgggg tggccgtacg
     2821 ctggagtggg caacctcttc cccgcctccg ttctataact ttgccgttgt gccgcacgtt
     2881 cacgagcgtg atgcattctg ggaaatgaaa gagaaaggcg aagcgtataa aaagcctgac
     2941 cactatgaag aaattcatat gccgaaaaac agcggtgcag gtatcgtcat tgcagctttc
     3001 tccaccatct tcggtttcgc catgatctgg catatctggt ggctggcgat tgttggcttc
     3061 gcaggcatga tcatcacctg gatcgtgaaa agcttcgacg aggacgtgga ttactacgtg
     3121 ccggtggcag aaatcgaaaa actggaaaac cagcatttcg atgagattac taaggcaggg
     3181 ctgaaaaatg gcaactgata ctttgacgca cgcgactgcc cacgcgcacg aacacgggca
     3241 ccacgatgca ggcggaacca aaatcttcgg attttggatc tacctgatga gcgactgcat
     3301 tctgttctca atcttgtttg ctacctatgc cgttctggtg aacggcaccg caggcggccc
     3361 gacaggtaag gacattttcg aactgccgtt cgttctggtt gaaactttct tgctgttgtt
     3421 cagctccatc acctacggca tggcggctat cgccatgtac aaaaacaaca aaagccaggt
     3481 tatctcctgg ctggcgttga cctggttgtt tggtgccgga tttatcggga tggaaatcta
     3541 cgaattccat cacctgattg ttaacggcat gggtccggaa cgcagcggct tcctgtcagc
     3601 gttctttgcg ctggtcggca cgcacggtct gcacgtcact tccggtctta tctggatggc
     3661 ggtgctgatg gtacaaatcg ctcgtcgcgg cctgaccagc actaaccgta cccgcatcat
     3721 gtgcctgagc ctgttctggc acttcctgga tgtggtttgg atctgtgtgt tcactgttgt
     3781 ttatctgatg ggggcgatgt aatgagtcat tctaccgatc acagcggcgc gtcccatggc
     3841 agcgtaaaaa cctacatgac aggctttatc ctgtcgatca ttctgacggt gattccgttc
     3901 tggatggtga tgacaggggc tgcctctccg gccgtaattc tgggaacaat cctggcaatg
     3961 gcagtggtac aggttctggt gcatctggtg tgcttcctgc acatgaatac caaatcagat
     4021 gaaggctgga acatgacggc gtttgtcttc accgtgctaa tcatcgctat cctggttgta
     4081 ggctccatct ggattatgtg gaacctcaac tacaacatga tgatgcacta agagcggcgg
     4141 ttatgatgtt taagcaatac ctgcaagtaa cgaaaccagg catcatcttt ggcaacctga
     4201 tctcggtgat tgggggattc ctgctggcct caaagggcag cattgattat cccctgttta
     4261 tctacacgct ggttggggtg tcactggttg tggcgtcggg ttgtgtgttt aacaactaca
     4321 tcgacaggga tatcgacaga aagatggaaa ggacgaagaa tcgggtgctg gttaaaggcc
     4381 tgatctctcc tgctgtctcg ctggtgtacg ccaccttgct gggtattgct ggctttatgc
     4441 tgctgtggtt tggcgcgaat ccgctggcct gctggctggg ggtgatgggc tttgtggttt
     4501 atgtcggcgt ctatagcctg tacatgaaac gccactcagt ctacggcaca ttgattggtt
     4561 cgctctccgg cgctgcgccg ccggtgattg gctactgtgc agtaaccggc gagttcgata
     4621 gcggcgcagc gatcctgctg gctatcttca gcctgtggca gatgcctcac tcctatgcca
     4681 tcgccatttt ccgctttaag gattaccagg cggcaaacat tccggtattg ccagtggtaa
     4741 aaggcatttc ggtggcgaag aatcacatca cgctgtatat catcgccttt gccgttgcca
     4801 cgctgatgct ctctcttggc ggttacgctg gatataaata tctggtggtc gccgcggcgg
     4861 ttagcgtctg gtggttaggt atggctctgc gcggttataa agttgctgat gacagaatct
     4921 gggcgcgcaa gctgttcggc ttctctatca tcgccatcac tgccctctcg gtgatgatgt
     4981 ccgttgattt tatggtaccg gactcgcata cgctgctggc tgctgtgtgg taacaaaacc
     5041 tctcagttaa aaaggtgcta cggcaccttt tttcttagca ttagaaacat atccctctcg
     5101 aaatatttac taaaaaatcc gcatgtttac cccattcgtt tgccgcttta cactagtcgc
     5161 gaatttaaaa cagaggtggt aatgaacgat tataaaatga cgccaggtga gcggcgcgcg
     5221 acctggggtt taggaaccgt tttctcgttg cgcatgctgg gcatgtttat ggttctgccg
     5281 gttctgacca cgtatggcat ggctctgcaa ggtgccagcg aagcattaat cggtattgcc
     5341 attggtattt atggtctgac tcaggccgtt tttcagattc cgtttggcct gctttccgac
     5401 cgcattggtc gcaaaccatt aattgtcggt gggctggcgg tgtttgccgc cggtagcgtt
     5461 atcgctgcgc tttctgactc catctgggga attattctgg gccgggcgct acaaggctcc
     5521 ggtgcgattg ccgctgccgt tatggcgctg ctttccgatc tcacgcgcga acaaaaccgt
     5581 accaaagcaa tggcgtttat cggcgtgagc tttggcatta cctttgccat tgcgatggtg
     5641 cttggcccga tcatcactca caaacttggg ctgcacgcgc tgttctggat gatcgctatt
     5701 ctggcaacga ccggcattgc gttgaccatt tgggttgtgc ccaacagtag cactcacgta
     5761 cttaatcgtg agtccggaat ggtgaaaggc agtttcagta aagtgctggc ggaaccgcgg
     5821 ctgctgaaac tcaactttgg cattatgtgt ctgcatattt tgctgatgtc gacgtttgtt
     5881 gccctgcccg gacaactggc tgatgcaggg ttcccggcgg ctgaacactg gaaggtctat
     5941 ctggcgacaa tgctaatcgc ctttggctcg gtcgtgcctt tcattatcta cgctgaagtt
     6001 aagcgcaaaa tgaagcaagt ctttgtcttc tgcgtcgggt tgatcgtgat tgcggaaatt
     6061 gtgttgtgga acgcgcaaac gcagttctgg caactggtgg tcggcgtgca gcttttcttt
     6121 gtggcgttta atttgatgga agccctcctg ccctcactta tcagtaaaga gtcgccagca
     6181 ggttacaaag gtacggcgat gggtgtttac tccaccagcc agtttcttgg cgtggcgatt
     6241 ggcggttcgc tgggcggctg gattaacggc atgtttgacg gtcagggggt atttctcgct
     6301 ggcgcaatgc tggccgcagt gtggctgaca gtcgccagta ccatgaaaga accgccgtat
     6361 gtcagcagtt tgcgcattga aatcccggcg aacattgccg caaacgaggc gttaaaagtg
     6421 cgtttgctag aaactgaagg catcaaagaa gtgttgattg cagaagaaga acattcagct
     6481 tatgtgaaaa tcgacagcaa agtgacgaat cgctttgaga tagaacaggc aattcgtcag
     6541 gcataaaaaa cgcggcgaat tgcctgctgc gctacgctta tcaggcctac aaagtttctg
     6601 aattacaaac ctttgtaggc cgaatcaggc gttcatgccg catctgacat gaacaaaacg
     6661 cacatagtcg cgattaatcg cggaagtttt tgaactggaa cggctgaccg agatcgccac
     6721 cacgtaccat cgccatgaca gcctgcaaat catcacgaga tttgcccgtt acgcggattt
     6781 catcgccctg aatttgcgcc tgcactttca gtttgctgtc tttgatcatc ttgacgattt
     6841 tcttctgggt cgcgctctca atgccctgtt tcagtttcgc ttccacaaac caggttttac
     6901 cgctatgaac gatattctcc ggtacatcca gcgaactgcc ttcaatgccg cgcttcagca
     6961 gcttggcacg cagaatatcc agcaactgat tgacctggaa atcggactcg ctcaacactt
     7021 tgatggtttt gctggcgtcg ttcagctcaa atgaggcttc aacgttacgg aagtcaaaac
     7081 gggactccac ttcgcggctc gcgttatcga ccgcgttacg tgcttcctga agatcaactt
     7141 cagagacaat atcgaaagat ggcatctttt cctctccctt catttttgat gcgaagcata
     7201 atacccgcaa agttaaataa ccgacagtcc tgcaccagga cgtatccctt aattgtgcat
     7261 agtcgcaaca gcatgcacaa ttaagggata gccttattga acacccggag tggttgcggg
     7321 tgaggaggaa caatgaaaat taccgtattg ggatgcggtg ccttagggca attatggctt
     7381 acagcacttt gcaaacaggg tcatgaagtt cagggctggc tgcgcgtacc gcaaccttat
     7441 tgtagcgtga atctggttga gacagatggt tcgatattta acgaatcgct gaccgccaac
     7501 gatcccgatt ttctcgccac cagcgatctg ctcctggtga cgctgaaagc atggcaggtt
     7561 tccgatgccg tcaaaagcct cgcgtccaca ctgcctgtaa ctacgccaat actgttaatt
     7621 cacaacggca tgggcaccat cgaagagttg caaaacattc agcagccatt actgatgggc
     7681 accaccaccc atgcagcccg ccgcgacggc aatgtcatta ttcatgtggc aaacggtatc
     7741 acgcatattg gcccggcacg gcaacaggac ggggattaca gttatctggc ggatattttg
     7801 caaaccgtgt tgcctgacgt tgcctggcat aacaatattc gcgccgagct gtggcgcaag
     7861 ctggcagtca actgcgtgat taatccactg actgccatct ggaattgccc gaacggtgaa
     7921 ttacgtcatc atccgcaaga aattatgcag atatgcgaag aagtcgcggc ggtgatcgaa
     7981 cgcgaagggc atcatacttc agcagaagat ttgcgtgatt acgtgatgca ggtgattgat
     8041 gccacagcgg aaaatatctc gtcgatgttg caggatatcc gcgcgctgcg ccacactgaa
     8101 atcgactata tcaatggttt tctcttacgc cgcgcccgcg cgcatgggat tgccgtaccg
     8161 gaaaacaccc gcctgtttga aatggtaaaa agaaaggaga gtgaatatga gcgcatcggc
     8221 actggtttgc ctcgcccctg gtagtgaaga gactgaagcc gtcaccacta tcgatctgct
     8281 ggttcgcagc ggtatcaaag tcaccactgc cagcgtcgcc agcgatggta acctggcgat
     8341 tacctgctcg cgcggcgtga agctgctggc ggatgcgccg ctggtcgaag tggctgatgg
     8401 cgaatatgac gtgatcgtgc tgcctggtgg cattaaaggc gcggagtgtt ttcgcgatag
     8461 cactctgctg gttgaaaccg ttaaacagtt ccaccgttcc gggcgtatcg tcgcggctat
     8521 ttgcgccgcg ccagccaccg tgctggtgcc gcacgatatc ttcccgattg gtaatatgac
     8581 cggcttcccg acgctgaaag acaaaattcc cgccgaacaa tggctggaca agcgcgtcgt
     8641 ctgggatgca cgggtaaaat tgctgaccag ccaggggccg ggtacagcta tcgactttgg
     8701 tctgaaaatt atcgacctgt tggttgggcg tgaaaaagcc catgaagtgg catcacaact
     8761 ggtgatggcg gcagggattt ataattatta cgagtagtgt cggatgcggc aaacgtcgca
     8821 tctgaccaga tgcgacgtaa aaaccacaaa ttacgggcga tataccttca cattgttaaa
     8881 gccctgctcg cgcagataga gcgcctgcag acggctcatc accccgcgct cacaccacag
     8941 cagccaagtt ttgttctggt cgagatcgcc aaatttggtg ctcagtttat agaacggcag
     9001 agaaaccaca tcaatccctt cgactttcag tggcttatct tcctgttcat cgatagaacg
     9061 gatatcgagg atcacgtcgt tcgggccgaa gccattgacg gtttccactt ccaccacttc
     9121 ctgctcggtc tgctgggcga tttcgcggat atcaacgtta ttcgcctcct caaccacttt
     9181 atcgagaatg ctgaagtcga acttctcttc ttccgcttca atcttcgatt taaccgcttt
     9241 caccgtcggg cttttggaga tcacgccaca atattccggc atcgtgcgag caaagtcttc
     9301 agtgccaatc tggcgggcca ggttgatgat gtgctctttg tcgtaagaaa tcagcggacg
     9361 caggatcagc gtatcagaga cgttatcaat caggcgcagg ttggtcagcg tctgactgga
     9421 cacctggccg agcgcttcgc cggtgaccag cgcctgtacg ccgtaacgtt cagccacttt
     9481 agacgcggca cgtaccatca tacgtttgag gataacgccc atctgaccgt cgtcgatttt
     9541 ctcgagaatt tccccgacga ccggttcgaa attaatagcg acaaaacgca cgcggtggga
     9601 gctgccaaaa cgattccaca gataatgcgc cacctgacgc acgccaattt catgcgccgc
     9661 gccgccgagg ttaaagaagc agtaatgcac gcggcagccg cgacgcatca acatataact
     9721 ggaaacaccg gagtcgaaac caccggaaat gagcgacagc acatcttcct gggtgccgat
     9781 cgggaaaccg ccaatacctt cgtagcggcc tttaatcagc aggagacgat cgtcttccac
     9841 ttccagatgg acagtcactt ccggattggt cagcttcacg cgcgcggact caatatgctg
     9901 atttaaaccg ccgccgacgt aacgttccac atcaatcgag ctaaaatcat gtttgccacg
     9961 gcgcttcacg cgtacgcaga aggttttgcc ttccagctga tcgcgatact gaaccaacgc
    10021 tttctcgaaa atatcgtgca tgtcggtaaa cggcacgtct tcgacttcga gaatatggtg
    10081 gatacccgga atacgggtca gggcgtcgcg aatagccaga cgctggtttt catcttttgc
    10141 gcgaacttcg atgttatccc agtggcggac gacagcgagc gtctcatcat agtgctttaa
    10201 aacgttacga atgttcccgg taaggatttt tataaagcgc aagcgcacag attggctttt
    10261 gatggtgatt tccgggaaca atttaatgat aaacttcatg gcggcaatgg ttcgttggca
    10321 agccttaagc gacttgtata gggaaaaata cagcagccca cacctgcggc tgcatccagg
    10381 cgcggaagta taccactaac atcgctttgc tgcgcacatc accttaccat tgcgcgttat
    10441 ttgctatttg ccctgagtcc gttaccatga cggggcgaaa aatattgaga gtcagacatt
    10501 cattatgccg aagaaaaatg aggctcccgc cagctttgaa aaggcgctga gcgagctgga
    10561 acagattgta acccgtctgg aaagtggcga cctgccgctg gaagaggcgc tgaacgagtt
    10621 cgaacgcggc gtgcagctgg cacgtcaggg gcaggccaaa ttacaacaag ctgaacagcg
    10681 cgtacaaatt ctgctgtctg acaatgaaga cgcctctcta acccctttta caccggacaa
    10741 tgagtaatgg actttccgca gcaactcgaa gcctgcgtta agcaggccaa ccaggcgctg
    10801 agccgtttta tcgccccact gccctttcag aacactcccg tggtcgaaac catgcagtat
    10861 ggcgcattat taggtggtaa gcgcctgcga ccttttctgg tttatgccac cggtcatatg
    10921 ttcggcgtta gcacaaacac gctggacgca cccgctgctg ccgtagagtg tatccacgct
    10981 tactcattaa ttcatgatga tttaccggcg atggatgatg acgatctgcg tcgcggtttg
    11041 ccgacctgcc atgtgaagtt tggcgaagcg aacgcgattc tcgctggcga cgctttacaa
    11101 acgctggcgt tctcgatttt aagcgatgct gatatgccgg aagtgtcgga tcgcgacaga
    11161 atttcgatga tttctgaact ggcgagcgcc agcggtattg ccggaatgtg tggtggtcag
    11221 gcgttggatt tagacgcgga aggcaaacac gtacctctgg acgcgcttga gcgtattcat
    11281 cgtcataaaa ccggcgcatt gattcgcgcc gccgttcgcc ttggcgcatt aagcgccgga
    11341 gataaagggc gtcgtgctct gccagtactc gacaagtacg cagagagcat cggccttgcc
    11401 ttccaggttc aggatgacat cctggatgtg gtaggagata ctgcaacgtt gggaaaacgc
    11461 cagggtgccg accagcaact tggtaaaagt acctaccctg cacttctggg tcttgagcaa
    11521 gcccggaaga aagcccggga tctgatcgac gatgcccgtc agtcgctgaa acaactggct
    11581 gaacaatcac tcgatacctc ggcactggaa gcgctagcgg actacatcat ccagcgtaat
    11641 aaataaacaa taagtattaa taggcccctg atgagttttg atattgccaa atacccgacc
    11701 ctggcactgg tcgactccac ccaggagtta cgactgttgc cgaaagagag tttaccgaaa
    11761 ctctgcgacg aactgcgccg ctatttactc gacagcgtga gccgttccag cgggcacttc
    11821 gcctccgggc tgggcacggt cgaactgacc gtggcgctgc actatgtcta caacaccccg
    11881 tttgaccaat tgatttggga tgtggggcat caggcttatc cgcataaaat tttgaccgga
    11941 cgccgcgaca aaatcggcac catccgtcag aaaggcggcc tgcacccgtt cccgtggcgc
    12001 ggcgaaagcg aatatgacgt attaagcgtc gggcattcat caacctccat cagtgccgga
    12061 attggtattg cggttgctgc cgagaaagaa ggcaaaaatc gccgtaccgt ctgtgtaatc
    12121 ggcgatggcg cgattaccgc aggcatggcg tttgaagcga tgaatcacgc gggcgatatc
    12181 cgtcctgata tgctggtggt cctcaacgac aacgaaatgt cgatttccga aaatgtcggc
    12241 gcgctcaaca accatctggc gcagctgctt tccggtaagc tttactcttc actgcgcgaa
    12301 ggcgggaaaa aagttttctc tggcgtgcca cccattaaag agctactcaa acgtaccgaa
    12361 gaacatatta aaggcatggt agtccctggc acgttgtttg aagagctggg ctttaactac
    12421 atcggcccgg ttgacggtca cgatgtgctg gggcttatca ccacgctgaa gaacatgcgt
    12481 gacctgaaag gcccgcagtt cctgcatatc atgaccaaaa aaggtcgtgg ttatgaaccg
    12541 gcagaaaaag acccaatcac tttccacgcc gtgcctaaat ttgatccctc cagcggttgt
    12601 ttgccgaaaa gtagcggcgg tttgccgagc tattcaaaaa tctttggcga ctggttgtgc
    12661 gaaacggcag cgaaagacaa caagctgatg gcgattactc cggcgatgcg tgaaggttcc
    12721 ggtatggtcg agttttctcg taaattcccg gatcgctatt ttgatgtagc gattgccgag
    12781 caacacgcag tgacctttgc tgcgggactg gccattggtg gatacaaacc cattgtcgcg
    12841 atctactcca ccttcctgca acgcgcctat gatcaggtgc tgcatgacgt ggcaattcaa
    12901 aaactcccgg tcctgttcgc catcgaccgc gcgggcattg ttggtgctga cggtcaaacc
    12961 catcagggtg cttttgatct ctcttacctg cgctgcatac cggaaatggt cattatgacc
    13021 ccgagcgatg aaaacgaatg tcgccagatg ctctataccg gctatcacta taacgatggc
    13081 ccgtcagcgg tgcgctaccc gcgtggcaac gcggtaggcg tggagctgac gccgctggaa
    13141 aaactgccaa ttggcaaagg cattgtgaag cgtcgtggcg agaaactggc gatccttaac
    13201 tttggtacgc tgatgccaga cgcggcgaaa gtcgctgaat cgctgaacgc cacgctggtc
    13261 gatatgcgtt ttgtgaaacc gcttgatgaa gcgttaattc tggaaatggc cgccagccat
    13321 gaagcgctgg tcaccgtaga agaaaacgcc attatgggcg gcgcaggtag cggagtgaac
    13381 gaagtgctga tggcccatcg taaaccagta cccgtgctga acattggtct gccggacttc
    13441 tttattccgc aaggaactca ggaagaaatg cgcgccgaac tcggcctcga tgccgccggt
    13501 atggaagcca aaatcaaggc ctggctggca taatccctac tccactcctg ctatgcttaa
    13561 caaattattc atagactcta aataattcga gttgcaggaa ggcggcaaac gagtgaagcc
    13621 ccaggagctt acataagtaa gtgactgggg tgaacgaatg cagccgcagc acatgcaact
    13681 tgaagtatga cgagtatggc aggagtggca gcatgcaata caacccctta ggaaaaaccg
    13741 accttcgcgt ttcccgactt tgcctcggct gtatgacctt tggcgagcca gatcgtggta
    13801 atcacgcatg gacactgccg gaagaaagca gccgtcccat aattaaacgc gcgctggaag
    13861 gcggcataaa tttctttgac accgccaata gctattccga tggcagcagc gaagagatcg
    13921 tcggtcgcgc actgcgggat ttcgcccgtc gtgaagacgt ggtcgttgcc accaaagtat
    13981 tccatcgcgt tggtgatttg ccggaaggat tatcccgtgc gcaaatattg cgctctatcg
    14041 acgacagcct gcgccgtctc ggcatggatt atgtcgatat cctgcaaatt catcgctggg
    14101 attacaacac gccgatcgaa gagacgctgg aagccctgaa cgacgtggta aaagccggga
    14161 aagcgcgtta tatcggcgcg tcatcaatgc acgcttcgca gtttgctcag gcactggaac
    14221 tccaaaaaca gcatggctgg gcgcagtttg tcagtatgca ggatcactac aatctgattt
    14281 atcgcgaaga agagcgcgag atgctgccac tgtgttatca ggagggcgtg gcggtgattc
    14341 cgtggagccc gctggcgcgg gggcgactga cgcgtccgtg gggagaaact accgcacgac
    14401 tggtgtctga tgaggtgggg aaaaatctct ataaagaaag cgatgaaaat gacgcgcaga
    14461 tcgcagagcg gttaacgggc gtcagtgaag aactcggtgc aacacgagca caagttgcgc
    14521 tggcctggtt gttgagtaaa ccgggcattg ccgcaccgat tatcggtaca tcgcgggaag
    14581 aacagcttga tgagctattg aacgcggtgg atatcacttt gaagccggaa cagattgccg
    14641 aactggaaac gccgtataaa ccgcatccgg tagtaggatt taaataagag tccaggcctg
    14701 atgagacgca acaagcgtca catcaggcat cggtgcacaa ctacgacaga atgcccagcg
    14761 gccagtgatg accaataaag tacaggatgc cagcggagat caccccggcg acaatatcat
    14821 ctatcatgat ccccatgccg ccatgcacat tgcgatcaaa ccagcggatc ggccacggtt
    14881 tccacatatc cagaatacgg aaaatcacaa acccggcggc aacccactgc cagtcattgg
    14941 tcggcagcgc catgagcgtg atccacatac caataaattc gtcccagaca atgctgccgt
    15001 gatcgtgcac gcccatatct tttgcggtct gatgacaaag atagacgccg atacagatcc
    15061 ccagcatcac caccagcgag tagagctgcc agggcaaaaa ggtcatcaga taccagaacg
    15121 gaatcgctgc cagcgagccc atcgtcccag gaacgatcgg gcttaatcca cttccgaatc
    15181 cgacagcaag cagatgccac ggattactca tcttcaggcg acttttcgcg acatctttat
    15241 ggcgtggcaa aatggtcata tcctttccag tcaaacgtga caggttcgcc gtcacgaata
    15301 aaacaaagcc cttcgatatc ggcggtcatt tgcccgatac aggtaaacgg tacgccaagg
    15361 tgtccgagag caacatccag cgcgccacgg ttcagttccg gcaccgtgaa acacaactcg
    15421 taatcttcac cgccagagag cgcccagcgc agcgcctgtt ccggttcaac atggcgagaa
    15481 agcgcatcag aaaacggcag caatgccagg tcaatacgtg cgccgcagtc gctggctttc
    15541 acgatatgcc cgagatcgga aatcagaccg tcagagagat cgatagctga atttgccaga
    15601 tcgcgcagtg cctgcccctg taaaatacgc ggcgatggac ggagatgacg tttgatcaag
    15661 tagtcagcat ctttagcatc ggcaacctgc aaacggtttt gcaaaatcgc cagcccggcg
    15721 gcgctatcgc ccggtgtacc ggtcacatag atccagtcac ccggtttcgc cccagagcgc
    15781 gttaaggctc gtcccatcgg aacaaagccg tggataccca acgtcattga taatggccca
    15841 cgcgtggtat cgccgccaat gagttgcata tcgtaataat tgagaagatc aaacaaactg
    15901 tcgctgaagg actcaagcca cgcttcgtct acgtccggta aggttaatgc cagcgtcagc
    15961 caggccggat cggcccccat cgctgccaga tcgcttaggt tcaccgccag tgctttataa
    16021 gccagatcag caggatcgat atcagggagg aaatggttac ccgccaccag cgtatcagtg
    16081 ctgatcgcca gggtctgttt ctcggggata ttgagaagtg cgcaatcgtc gccgatgccc
    16141 agttcgacat caagacgaga acttcttaca cggtcaaaat aacgggcaat cagggagaac
    16201 tcgccacatg ccatacgtta tgcctcagca ggtaaaaaag aaaaggccgg cgatcatgga
    16261 acggtcttcc gtgaatctac cggcctggat atcacttttt gttagggcga atcacaggtg
    16321 ctgctttatc gagtacgccg ttgacgaact tatggctgtc ttctgcgccg aacgatttcg
    16381 ccagttcgat cgcttcgtta atggccactt tgtatggcac atcgctacgt ttagacagtt
    16441 cgtacagcgc aatgcgcagt actgcttttt ctacctgtcc cagttcttcc agcaggcggg
    16501 acaggtatgg cttcatcagt ccgtcgaggt atgcggtatt agtcgccacc ccggccagca
    16561 gctcacggaa gtacaggacg tcaacgtctt ttacatcctg ttcagccagg aactggtatt
    16621 caacatcagc gatgtcgttc tgggacaact gccaggagta gagcgcctgg acggcacact
    16681 cacgagcgcg gcgacgagca gcaggtttca cggatttccc cttactaatt tcaggccttg
    16741 atggctttca atacattaat catttcaagc gcggtcagtg cagcttctgc acctttgttg
    16801 ccagctttgg tgccagcacg ttcgatcgct tgttcaatgc tttcagtggt cagaacccca
    16861 aaagcaaccg gaatttcgct gtcctgggca acatgcgcca ggccgttgct tgcaccacca
    16921 gcgacatatt caaagtgggc agtgccacca cgaataaccg tacccagcgc aatcaccgcg
    16981 tcgtatttac cggttttagc cagtgcaccc gccgccagcg gcagctcata ggcaccaggc
    17041 acccaaacaa cggtaatgtt ttcatctttt acctgaccga tacgtttcag tgcgtcaatt
    17101 gcaccttcca gcaggctgtc attgataaag ttgttgaaac gcgcgatggt gatggcgacg
    17161 cgagcgtccg gggtagcaac gttagcttca ataatgttca ttttcttcct tcgggttcga
    17221 gtatggcccc gcaggggggc ggattttagc ataatatttc gtgcgctgct tccctttcga
    17281 gccgggagat catgcaccta ctaaatgcag gcaaacatcc gggcctacat gacgtatctc
    17341 tttgaattta aattgggggg cgtcggctaa tttctcaagc cctggcagcg agcataatcc
    17401 acgggcgtcg ctgcctaata gtttaggtgc gatatagaca atcagctcat cgactaaacc
    17461 cgcctgcagc aatgcgccag cgagcgttgg ccccgcttcc acccagatgc tgttaatttg
    17521 ctgtttaccc agttgcatca tcagtacaac cagatccaga tgacctttat gcgctggaat
    17581 cagcaaggta cgcaccgttt ccggccactc acgagaatct tcctgcgtac gcgcgaacca
    17641 ggtttcgccg ggctgctgca caatgcgatg taccggcgtc acgcgatttt ggctatcaat
    17701 cacaatacgt atcggctgac ggagattttg ttgcggatag agcgcctgag tttgttcatc
    17761 cagttcagac caacgcaccg ttaaggcagg atcatccgcc agcaccgtgg cgctgctggt
    17821 taaaatggca tgactttgcg cgcgcagtag ttgtacatca cgccgcgcct ggggcgaagt
    17881 gatccactgg ctttcgccgc tcgccatcgc cgtgcgacca tcaagcgatg cgccaagttt
    17941 taactgaata taaggaaagc cggtgcgcat ccgcttgaga aagcctttat tcaattgctc
    18001 ggcttcactc atcatcaggc cgtggctgac gtcaatgcca gcctgttgca gacggtaaag
    18061 tccacgccca gcgacctgcg ggttaggatc ttgcatcgag gcaaccacgc gcgctacgcc
    18121 agcggcgatg agtgcgtcac agcacggtgg cgtacgacca tgatggctac agggttcgag
    18181 tgtgacatag gcggtcgcac ctttggcttt ttcacccgcc atacgcaacg cgtgtacttc
    18241 ggcatgtggt tcacccgcac gttggtggta accttcaccg acaatttcgc catctttgac
    18301 aatgacgcac ccgacattcg ggttgggatg cgtggtaaaa cgtcctcgtt gcgccagctt
    18361 tagcgcccgc gccatgtaat actcgtcctg cacggcttag tcctccaggc gcgcgatctc
    18421 ttcgccaaat tctttgatat cttcgaaact gcggtagaca gaggcaaaac ggatataggc
    18481 gactttatcg agctttttca attgctccat caccagattg ccaatcatct tgctcggcac
    18541 ttcgcgctca ccggtggcgc gcagctgcga tttaatatga ttgattgcca tttcgacgtc
    18601 atcggaactc accggacgtt tttccagcgc ccgcagcatt ccgctacgca atttctcttc
    18661 attaaacggt tcacgcacgt cgttgctttt tacaacacgc ggcataacca gctccgccac
    18721 ttcaaaggtg gtgaaacgtt cattacacac cagacactgc cgacggcggc gtacggatga
    18781 accctcgccc acgagacgag agtcaattac cttagtgtcc acggcgaaac agaatgggca
    18841 atgcatacgg cgtcctgacc aggtggttaa cagaaatcta ttttaccctg aactgacgat
    18901 acaacaaagg cgcaacgctt ttgagataaa ggatcgatgc tttcgctgct tttaccctct
    18961 accattaggc taattgcgac gagaaaggga cgacctatga atactaacgt ttttcgactg
    19021 ctcctgctgg gaagcctgtt cagccttagc gcctgtgtgc agcagagcga agtgcgacag
    19081 atgaaacaca gcgtcagcac gctgaaccaa gagatgacgc aactcaataa agaaacggtc
    19141 aaaatcactc agcaaaacag gctgaatgca aaatccagca gtggggttta ccttctgcca
    19201 ggagcgaaaa caccggcaag actggaaagc cagatcggta ctttacgtat gtcgctggtg
    19261 aatattacgc ctgatacaga tggcaccacc ctgacgctac gtattcaggg cgagtcgaat
    19321 gatcccttgc ccgcattcag cggaactgtt gaatatgggc aaattcaggg aacaatagac
    19381 aactttcagg aaatcaatgt gcagaatcaa ttgattaatg cccctgccag cgtccttgcc
    19441 cccagcgatg ttgatattcc gttacagtta aaggggattt ctgtggatca attaggcttt
    19501 gtacgtatcc acgacattca acctgttatg cattaaacgt attgcggggc aattttgtgc
    19561 gtcccgcaac atctttcccc gtcattttgt tactccgctt acatcaccca gattgatagt
    19621 aaaagtttgc aacaagggtg aaagtcagta caatccctgc ccgaatgtgt gtaaacgtga
    19681 acgcaatcga ttacgtaaat gatagaactg tgaaacgaaa catatttttg tgagcaatga
    19741 tttttataat aggctcctct gtatacgaaa tatttagaaa cgcaatttgc gcctttttca
    19801 ctcccgcaag ggattttcaa acagtggcat acatatgaaa aaaacattac tggcagccgg
    19861 tgcggtactg gcgctctctt cgtcttttac tgtcaacgca gctgaaaacg acaaaccgca
    19921 gtatctttcc gactggtggc accagagcgt taacgttgtc ggaagctatc acacccgttt
    19981 cggaccgcag atccgcaacg atacctacct tgagtacgaa gcattcgcta aaaaagactg
    20041 gttcgacttc tatggttatg cggatgcgcc ggtattcttc ggcggtaact ccgatgcaaa
    20101 aggtatctgg aaccacggtt ctccgctgtt tatggaaatc gaaccacgtt tctccatcga
    20161 caagctgacc aatactgacc ttagcttcgg tccgttcaaa gagtggtact tcgcgaacaa
    20221 ctacatttac gacatgggtc gtaataaaga tggtcgccag agcacctggt acatgggtct
    20281 gggtaccgat atcgacactg gcctgccgat gagcctgtcc atgaacgtct atgcgaaata
    20341 ccagtggcag aactatggcg cagcgaacga aaacgagtgg gacggttacc gtttcaaaat
    20401 taaatacttt gtgccgatta ccgatctgtg gggcggtcag ctgagctaca tcggcttcac
    20461 caacttcgac tggggttccg atttagggga tgacagcggt aacgcaatca acggtattaa
    20521 gacccgtact aataactcta tcgcttccag ccatattctg gctctgaact acgatcactg
    20581 gcactactct gtcgtagctc gttactggca cgacggtggt cagtggaacg acgatgcaga
    20641 actgaacttc ggcaacggca acttcaacgt tcgctctacc ggctggggtg gttacctggt
    20701 agtaggttac aacttctgat tatgaaaatg ccgggattta ttcccggcat ttctgattgt
    20761 taacaacgtg tgctttgttc atgccggatg cggcgtaaac gctttgtccg gccaacaaaa
    20821 gcacgtaaat tcaatatatt gctgaaataa cgtaggcctg ataagcgtag cgcatcaggc
    20881 aattttacgt tttaatcact tcttcttgtt catcatcgct ttcaggtcag cgaatgggtt
    20941 gtacttcgct tcaccgacat ctttctgcgc atcttcccct gcgataacgg tcgtaccata
    21001 ctgatccgct tcggtatatt tcgaatgctc atgatcgtgg caatagagac acaacaattc
    21061 ccagttactg ccatcttccg ggttattggt atggtcgtga tcaatgtggt gaacggtaag
    21121 ttcacgcagg ttggaataaa caaactcgcg ggaacagcga ccgcagaccc acggatagat
    21181 ttttaatgct ttttcgcgat agccgctttc taaccgcgca tagttttttg ggatgatagc
    21241 catgaaaagt tacctggatg aaatgtaggg ttacgcgcag tttcccaaga tgcggggatt
    21301 ttcgcaatct ccatacatca aaaaaatccc gatcttctga ccgggatttt caacatcaac
    21361 gggaacttga ttacggcaga attgacggct gatccgcccc ttctttttcc actttctgct
    21421 gcaacatgtg ttcgcgcttc atacccagtt tcagagccaa cgcagatgcc acatagatgg
    21481 aagatgcagt accgatggaa acaccgataa gcatggtcag cgagaagcct tccagtaccg
    21541 gaccaccgaa gaggtacagc atcaggataa ccatcaaggt agtaccggat gtgatcaagg
    21601 tacggtgcag cgtctgggtc aaggacacgt taaagatttc gtaaggcgta ccgcgacgga
    21661 tcttgcggaa gttttcacga atacggtccg ataccacgat actgtcgtta agcgagtaac
    21721 cgataaccga catcaacgat gccacaatgg tcaggtcaat ctcgatatgg aataacgaca
    21781 aaatacccag cgtaatgata acgtcgtgcg ccagcgcaat aaccacccct gccgccagtc
    21841 gccactcaaa gcggaaacct acgtacacga ggatagacag cagcgctgcc atcaacgcca
    21901 tcgcaccggt ttgcgcaagg tctgccccca cgctcggacc gacgaactca atacgcttca
    21961 ctgctgcatt ctgattggtg gattcgttaa tcaccttcag aacctggctg cccaacacct
    22021 gaccgccggt ttcgccttca gcaggcggca tacggaccat gatgtcatgg ctgctaccaa
    22081 agttttgcag catcggctct tcaaaaccgg ctttttgcaa tgcatcacgc attacgtcaa
    22141 tttcagccgg tttttcgagc gtaatttcaa taaccgtacc accggtgaaa tccagccccc
    22201 agttaaagcc gcgcacgccc ataataacga tagcggcgat taacagcaga ccagagatgc
    22261 cgaaagccca gtagtcccag cgcataaagt catagacttt acggccatgg tttagttgtt
    22321 caacagtata ttcctgtgcc acatcgcact cctcagattg acagcttctt gacgcgcttg
    22381 ccgccatata gcaggtttac gatggcacgc gtaccgacaa tcgcggtaaa catcgacgtc
    22441 gccacaccga taccggtagt aatcgcgaac cctttaattg ccccggtacc cactgcgtac
    22501 aggatgatga ctttaatcag cgtggtgatg ttcgcatcga agatagaact gaatgcgcca
    22561 cgataacctt catcaattgc ctgttgaaca gtacgtccgt tgctcaactc ttctttaata
    22621 cgttcgttga taagtacgtt cgcatcgacc gctaccgcaa gtgttaagac gatacccgcg
    22681 atacctggca tactcagcgt tgcgcctggc aacagcgaca taatgccgac gatcaagatc
    22741 aagttggcaa tcagagcact ggtcgcaatc agaccaaatt tcttatagaa gatgatcatg
    22801 aacagaatag aaaccagcag accggcaagg caagcctcca gcccctgctc aatgttctgc
    22861 atacccaggg tcgggccaat ggtacgttct tcaacaatct gaatcggcgc gatcaacgca
    22921 ccggcacgca gcagcagtga cagctgacgg gcttcgttcg ggttgttgat gccggtgata
    22981 cggaagctgt tacccagacg agactggatg ttggcgatgt taatcacctc ttcctgtttc
    23041 accagaaccg cacgaccatt tgcatctttc ttaccgctgt ctttgtactc cacaaacagg
    23101 gttgccatcg gtttgccgat gttgtcctta gtgaagttag acatgatgtt accaccagcg
    23161 ctatcgagcg agatgttaac ctgcggttgg ttgtattcgt cctggctgga agtggagtcg
    23221 gtgatatggt caccggtcag aattacgcgt ttgtacagca caactggctg accttcgcgg
    23281 gtctgtttca cttcagagtc gcccggtacg cgaccggatg ccgcagcggc ctggtcaacg
    23341 ttggtgttta ccagacggaa ttccagcgtt gccgtcgcac ccagaatctc tttcgcacgc
    23401 gcagtgtcct gaatacctgg cagttcaaca acgatacggt cagcaccctg acgctgaacc
    23461 accggctccg ccacgccaag ttggtttaca cggttacgca ggatattgat gttctgctgc
    23521 accgcatatt cacgcgcttc actcagacga gcatcgctca ttaccgcacg cagctggttg
    23581 ctgccctggc tactaatcac caggtccgga tggcgcttgc tcagatacgc aatggcttca
    23641 tcacgagctt tagcatcgcg gaaagtaatg ctcaggccgt agttgttttc tttacgaacg
    23701 gtggtatacg ggatgccctt ttcacgcaga tcgctgcgca ggctatcgat attttgttcc
    23761 tggagtttgc caagcgcggt gtccatatcc acttccatca ggaagtgaac gccgccacgc
    23821 aggtcaaggc cgagcttcat cggctcagcg tgaatagctg ccagccagcg cggcgttgcc
    23881 ggggcaaggt taagcgccac gacgtatttg tcacccataa cgcccattaa tgcttcacga
    23941 gcgcgcagct gggtgtcagt ggagtcgaag cgcgcaagaa tagcgccctc ttccagtgcc
    24001 acagacttag cagttatttt ttcttcttgt aacgttttct ggacctggat cagcgtttgc
    24061 tcactggcgg cgactccgcg cgcaccagtg atctgaacag ccggatcctc accaaacagg
    24121 ttgggaagcg catacagcag accgatgaca atcaccacga tcagcatgac gtacttccac
    24181 aaaggataac ggtttaacac ggcaattccc ttagggaaaa attttaatta cagcgccttc
    24241 atggtgcctt tcggcaggac ggcagctacg aagtcacgtt taataactac ttcagtggtg
    24301 tcattcagcg cgatagcaat gtagccgttt tccgctactt tggttacgcg accaaccagg
    24361 ccaccgttcg tcagaacttc atcacctttg gcaatggagt ccatcagctt tttgtgttct
    24421 ttggtgcgct tctgctgtgg acgcaggatc atgaaataga aaatcagacc gaataccacc
    24481 agcatcaaaa tcaaagacat cgggctacct tgcgccggtg cacccgttgc cgctaccgca
    24541 tcagaaataa aaaagctcat taaatttccc tcattattaa tattaatcaa cgttcaaagg
    24601 tggtacttct cgcccctgac gctggtaaaa atcagttacg aagctctcta atttaccctc
    24661 ttcaatagcc ttgcgtaaac ccgccatcaa acgctggtag taacgaaggt tatgaatggt
    24721 gttgagtcgc gcgcctaata tttcgttgca acggtcaaga tgatgcaagt aagcgcgtga
    24781 ataattgcga caggtgtagc aatcacactc aggatcgagt gggccagtat cgctcttata
    24841 cttcgcattg cggattttca ccacgccatc ggtcacgaac aaatgaccat ttcgggcgtt
    24901 gcgggttggc attacgcagt caaacatatc gataccacga cgtacgcctt caaccaggtc
    24961 ttctggttta ccaacgccca tcaggtaacg cggtttgtct gccggaattt gcgggcatac
    25021 atgctccaga atgcggtgca tatctgcttt cggctcaccc acagccagac cgccgacagc
    25081 gtagccatca aaaccgatat ctaccagacc tttaacagaa atatcacgta aatcttcgta
    25141 aacgctgccc tggatgatac caaacagcgc atttttgttt ccgagactgt caaaacgctc
    25201 acggctacgc ttcgcccaac gcagagacat ctccatggag cgttttgcgt aatcccagtc
    25261 agcaggatac ggcgtacact catcaaagat catgacgata tccgaaccaa gatcgtactg
    25321 aatctccatt gatttttcag gatcgaggaa aatcggatcg ccgttgatcg ggttacggaa
    25381 gtgcacgccc tgttcggtga ttttacgaat atcgccaagg ctgaagacct ggaagccgcc
    25441 ggagtcggtg aggatcggcc ccttccactg cataaaatcg tgcagatcgc cgtgcagttt
    25501 catgatttcc tggcccgggc gcagccacag gtggaaggtg ttgccgagga taatttgcgc
    25561 gccagtggct tcaacttctt ccggcgtcat cccttttacg gtgccgtagg tgccaacagg
    25621 cataaaacaa ggcgtttcca ctacgccacg atcaaagacc aggcggccac ggcgtgcgcg
    25681 accgtcggtg gtgtccagtt caaatttcat tttttctcca ctacgtcaga aaaacagtcc
    25741 aacgttttaa accagcgccg cggaattact ccccgacgcg ctcattaatt gcctgcggat
    25801 tgtacgtgat aaacatcgca tcaccgtaac taaaaaagcg atatttctct tctaccgctg
    25861 ctttataggc gttcatggtg tgttgataac cggcaaaggc cgaaaccagc ataatcagcg
    25921 tcgactctgg caagtggaag ttcgtcacca gcgcatcgac cactttgtac tggaagcccg
    25981 gatagataaa gatttgggta tcgtcgaaga acggttcaat gagatcgttt ttcgctgcct
    26041 gagccgcgct ttccagcgaa cgtactgaag tggtgccaac cgcaatcacc cggttaccgc
    26101 gcgctttcgc cgccagtacc gcgtctacca catcctgcgg tacttcagcg tattccgagt
    26161 gcatgatgtg atcttcaatg gtgtcgacgc gcaccggctg gaaggtgccc gcaccaacgt
    26221 gcaacgtcac aaacgccatc tccacgcctt tggcgcgcaa tttttccagc aaaggctcgt
    26281 caaaatgcag acctgcggtc ggggctgcaa ccgcgcccgg tttttcgcta taaacggttt
    26341 gataaagttc gcggtcagcg tcttcgtccg gacggtcgat atacggcggc agcggcatat
    26401 ggccgatgct gttgagaata tccagcaccg agcgttcatc attaaattcg acttcaaaca
    26461 gtgcgccgtg gcgcgcggtc attgttgcgt taatactttc gtcatcgccc agcagcagtt
    26521 ctgcgccagg ttttggcgct ttcgaggcgc gaatatgcgc aagaatgcgt ttgtcgtcga
    26581 gcatccgttc aaccagcact tcaatcttgc cgccgctggc tttacgccca aacaggcgcg
    26641 ccgggatcac gcgggtatta ttaaaaacca gaagatcgcc ggggttgagc ttatcaagta
    26701 aatcggtgaa agtaccgtgc gtcagcgcgc ccgtcggccc gtccagcgac agtaaacgac
    26761 agctactgcg ttcaggcatg ggatagtggg caatcaggga ttcgggcaat tcaaaggaga
    26821 aatcggtaac gcgcatgaca ctgactcaga ctaaaataag aggcgggtag tctagtgccg
    26881 gggcgctctc cctgcaacct ttaccccctt ttggatagaa tataatcgat gaatttttta
    26941 gctcacctgc atttagccca tctcgcggaa agctcgcttt ccggcaattt actggctgat
    27001 ttcgtacgcg gaaatcctga agaaagtttt ccgcccgacg tcgtggctgg cattcatatg
    27061 catcgacgta tcgacgtatt gactgacaat ctgccggaag tccgcgaagc acgggagtgg
    27121 tttcgtagtg aaacgcgccg cgttgcgcct attacgctgg atgtcatgtg ggatcacttt
    27181 ctttcccgcc actggtcgca gctgtcgccg gactttccgc tacaggaatt tgtctgttat
    27241 gcccgcgagc aagtgatgac gattttgccg gactcaccgc cacgttttat caatctgaac
    27301 aattacttgt ggtcagagca gtggctggtg cgctatcgcg atatggattt catccagaac
    27361 gtgttaaacg gcatggcaag ccgccgccca cgtctggatg ccctgcgtga ctcctggtac
    27421 gatttagacg ctcattatga cgccctcgaa acccgcttct ggcagtttta tccgcggatg
    27481 atggcgcagg cgtcacgcaa ggcgttataa aacgttagtt catccatacc gtagccgaaa
    27541 tggcaggcaa agccagaatc ccgtcagtca gttgcccatg cccttctttg cattgccatt
    27601 gcacggcatt gagaaacggt gacgcgggta gcaccacttc acaggcctcg ccacggttga
    27661 ttgccaccag tacacgttgc tgattcagca cgcggacaaa taccaccacg ttatcttccg
    27721 catacagcac ctgacagccg ccatgacgta gcgcctgact tttcttacgc agcgcaatca
    27781 ttcgctggta cagcgcgaat aacgccgtat cctgcttttc cacctgccag gggaacggtt
    27841 tacggcaaaa cggatcgttt ttgccatcca gtcctacttc atcaccgtaa taaatgcacg
    27901 gtacaccagg ccaggtgaac agccagacca ccgccagcgg caggcgcgca atatcccgac
    27961 cgagcagcgt tttaaatcgc gcagtatcgt ggctgtcgag ctgattaaac atacgtaatt
    28021 gttgttgatg agaaagccct gcgcggtaat tatccatcca ggccatacag gtttgggcat
    28081 caatttgctg cggatcgtaa gagatatcgg tattggcaag aaatccccac aacgggaatg
    28141 tgaagccacg atagttcatg gcggcatctt ccacatcggc ctgtaaccat tgccgtgcat
    28201 cgccaaaatg ttcgccgaca atatacgctt ccggctgggt ttctttcgcc gcttcggtga
    28261 tcccagcaac gtgctgcata ttattgcgcg ccccacccgc ctcccccagc atatgcacca
    28321 catccagccg ccagccgtcc atactccacg gcgctttcag ccaatggcgg acaatactgt
    28381 cttccccgcg ataaatttca ttcaccagac tttccgactg ataatccagc ttcggcaagc
    28441 tggcatagcc aagccagtcg agcgccgtgc catcatcact aaacgagtac cagtcgcgcc
    28501 agggcgattc ggggttgtga caagctcccc ccgtgccacg attgtgcctg tcaaaccagg
    28561 catgggaatc gccactgtgg ttaaacacgc cgtccagcac cagccgcatt cccagctgct
    28621 gcgtattgtg tcgcaaacgc agcaacgccc catcaccgcc aaactgcgga tcgacatggc
    28681 gataatcctc ggtatcgtat ttatgtacgc tgggagcttt aaacaccgga ttgagataca
    28741 gcgctgtcac gccaagcttt ttcagatacg gcagtttttc gcttatcccg tccagatcgc
    28801 cgccatagaa cgttgatccg cccgcctgcg ccgtgaccgg ttcatcccag tcacgcaaga
    28861 tgatctcttg tccggctgca tgatggtaat agacatgatc ctgttcagct tcacgaggaa
    28921 gactacgcgc aaaacgatca gggaagatct gataaaaaat ctgatccgca gcccattgtg
    28981 ggccgatatc cggtacatcg acggcaaact gctccagtcg tgccggcggc attcggctga
    29041 agccctgcgg tgtaaaccaa cgctggcgat cgtgccacag cagtttgaaa ctgtaacgcc
    29101 gccggggttg tccgctggag agatcaatcg ccgcacgcca tgcggtgacg cctggctgcg
    29161 gctgactgcg ctgcttatgc attggtactg acatttcttc gttatcgtgt tctgtacgca
    29221 gcataatgcg ctgcggtggg tcttcgcccg tcagccacag tgttatgagc agttgatctt
    29281 tgctttgttt aacaaatggg ggcaccggca ggtgccatgc atttaacatc ataattcccc
    29341 tgtgatgaaa atctgatcac cttgccatag cctaatgcag gatcactcat catctgaagg
    29401 atatttgggg gaggaggacg ggggcggata actttcggaa tagccggatc aggatttcct
    29461 cctgaaccgg cttacagaac gcaagtatta ttggttttca gccagctgac gatcgtggcg
    29521 acgcttaaac atccagccaa tcaacagcac aacaatccac gcgaaaccga catacagcga
    29581 gatacgcgta tccgggtgat aaccaatcaa cccgataata aagagcagga aaatcagccc
    29641 gccgatggtc gttgctaccc cacccggcac tttaaatttc agcgccttaa cttcttctgg
    29701 cggcaaacgg cgacggaagg caatttgcga cagcaggatc ataatccaca cccacaccgt
    29761 ggcgaaggtt gccagcgaag cgatcaccag gaagacgttt tccggcatga tgtagttcag
    29821 atacaccgca aacagcagcg cggtagtcat caccagcacc gtaacccacg gaataccgcg
    29881 acgcgacgtt ttgctgaaaa ttttcggcgc gctgccctgc tctgccatac cgtggagcat
    29941 acggcctacg ccaaatacat cactgttaat tgccgacagc gaagcagtca gcacaacaaa
    30001 gttaagaatg ctggcggcaa aggtaatgcc catatgctgg aacgtcagca cgaacgggct
    30061 accggcagtg ccaacctgat tccacgggta gatagacata atgacgaaca gcgtaccgac
    30121 gtagaacacc agaatacgca tcggcacgga gttaatcgca cgcggtatcg atttctcagg
    30181 atctttcgct tcaccggcgg taatcccgat aatttcgatc ccaccgtaag caaacatcac
    30241 catttgcaac gacattacca tgccaagcca gccgttactg aagaagccgc cgttgctcca
    30301 caggttatga ataccggtcg gttgcccgcc gttgccaatc ccccagatga tgatgccgaa
    30361 accggcgaca atcatgatga tgatggtggc gactttaaag aacgagaacc agaattccag
    30421 ctcaccgaat accttcacgc tcatcaggtt tacggcgcaa atgatcagca ccacgctcag
    30481 tacccaaatc cagtgcggca ccgtcgggaa ccagacaccc atatagatac caaaagcggt
    30541 cacatcggcg atggcgacaa taaggatttc aaagcagtag gtccagccgg taatgtaacc
    30601 tgccagcggg ccgaggtttt cctgcgcata acgcgagaaa gagctggcgg ccgggttatg
    30661 taccgacatt tcccccagcg cacgcataat gatatacgcc gcgataccac cgataatata
    30721 ggccaacaac acgctcggac cggccatttt gatggcgtct gccgaaccgt aaaacagccc
    30781 ggtgccaatt gctgaaccca gtgccataaa gcgtatgtgg cgggtactta gcccacgctt
    30841 tagcttgttc ttactttcca tcaattccta acccatcaac acaataaaaa accacggggc
    30901 atcgcagccc cgtggttaaa acaaatgttc agtgatttag tgagcgctgg aggtcacctg
    30961 acgacctgcc gcgcgatccc agataatggc cagaaccacc atcaccactg ttggcattaa
    31021 ccacgccaga ccttgttcgg ccagcggtaa acgctgcgcc caggacggta agatatcgct
    31081 gaatgcagat gctttgatcc cgtcgagaat accaaaaagc aggctgataa acatcggcgg
    31141 agcaatcacg cgggacgaat tatgccacca tgagcgtgta aaacttaata caaccagtgc
    31201 gatacacggc ggataaatag cggtcagcac cggtacggag atctggatca gctggcttaa
    31261 gccgaggtta gaaaccacca tcgagaagcc gccgaggata aacaccagcg tacgataaga
    31321 gagcggtacg tactgggcaa agaattctgc acaagcacag gtcaggccaa ctgccgttac
    31381 caggcaggcg atgaagatta acgccgccag caggaagcta ccgccgccgc caaaggtgtg
    31441 ctgaacgtaa gcatgcagaa tagcagcgcc gtttgcagac tgatcgacca gcgacgcgct
    31501 gtctgaaccc agacggaaca gcgccaggta cagcagagtc agaccaacac ccgccatcag
    31561 gccagcccag acggtataac gggtcagtag gcgcgcttcg gtaacgccac gagaacgcgc
    31621 cgcgttaaca ataacgatac caaacaccat tgcgcccagc gtatccatgg tcagatagcc
    31681 gttaacgaaa ccgttagaaa acgcagcgtt ttgataagcc tcagtcgccg tgctgataga
    31741 acccgccggc cagataatcg cggcaacaga caggatgacc agcgcgataa ttttcagcgg
    31801 cgcaaggaag ttgcccacgg tatccagcag cttgcccgga tagagcgaaa ccagaataac
    31861 gatagcgaaa tagaccaggc tgtagataaa cagcggcagc gcggaatcac ccgtcagcgg
    31921 cgcaatcccc acttcaaaag aaacggtagc tgtacgcggc gtagcgaaaa gcggccccac
    31981 cgccaggtaa caaaccgttg ccagcagtac gccagcgact ttaccgattg gcgtgctgag
    32041 gctgtcaaca ccgccgccaa cttttgccag cgccactact gttaataccg gcaggccaac
    32101 ggcagtaatg aggaagccga atgccgcagt ccagacgtgt tcgcctgcct gtaagccgac
    32161 cattggaggg aaaataatgt tacctgcgcc gacgaacaac gcaaatgtca taaagcccag
    32221 agcgatgata tcgcgcgatc ttaattgatg ggtcataaat catactgcct gtggatgtgg
    32281 tgttgtaacg ttaaattttt gtgccaatcc ctggggatga tacagcggta tatttaaaca
    32341 attttagcgg gaaatactcc caaccaggcg cggcgaagaa tagtttttcg atttaccgcg
    32401 ttaatgcagt cagtatgaca gcacctgaag cgcaatttaa acgcttataa cgttttaaag
    32461 caaggaggga tctaaaaacc agaggtatat agcgataaga aatcaccaac cagtataaaa
    32521 cactatttcc gtgaaaaaga catggctaat catgcgaaca aaaaatcacc aaccgttgta
    32581 aaatctaacg cccgtatttt gactgcaaag aaaataagcc agcatcgact ggcttatggc
    32641 aataaaagat gacaaaggcg gattaatcgc tgtttttggc aattaaacgt tccgggataa
    32701 caaaactgaa acgtgttcct tttcctactg tactctcaat attcaggcga ctttcgtgat
    32761 gattcaccgc atgtttcacg atcgctaacc ctaatccgct accgccggtt tgccgggaac
    32821 gcgctttatc aacgcgataa aaacgctcgg tcaggcgcgg aatatgctcc ggtgcaatgc
    32881 ccggtccgtt atcttcaacg ctaaattcgg caccgtgcgg cactcgctgc cagcgtacgg
    32941 tgatatgcgt gccttccggc gtatgattca cggcgttata gaccaggttc gaaatcgcac
    33001 tgcgtagctg atcttcattg ccagacacct tgaggccgtt atctatctca aaggtaaatg
    33061 tctgtttttt ctgactcaga gtctgagcct cgcgctcaac aacgcgcagc atcatcggca
    33121 catcaacctt ttcattgagc aaatgcgtcg gcgcggcttc aattttcgac agcgtcagca
    33181 attgcttcac cagcccttcc atccgctggg tctgctcgcg catggtgtgc aacgcttttt
    33241 cgcgtaccgc gccttccagc ggctgctcat ccatcatctc caggtaaccc tgtaacacgg
    33301 tcaatggcgt gcgtaactca tggcttacgt tggcgaaaaa gttgcgccgc gccccttcca
    33361 gttgatgcat ttgcgtgaca tcacgcgcca ccatcagcaa ctgtttgtgg gtataaggca
    33421 tgacgcgaat ttccagatgc cgcccggtgt tgagcaccag attgagcggg cgagaaaaat
    33481 cacgcgtttt cagatattgc gtaaactccg ggtaacgcag taggttaagg atgttctgcc
    33541 cgttatcttc cggccagcgc aaaccaagaa tttgttgcgc cagaccgtta caccagaaaa
    33601 taccgccctc ttccgtggtc agcaccaccg cgtcgggcag cgactccgcg ccgctacgaa
    33661 agcgtttaat cagattgccc agttcacggc ggcgtttttt atttcgcagc tgcatctggt
    33721 gtaagccgta tagtagcggt tcccagctac cacgccccgg tggcggggtc atactgcgat
    33781 ccacccacag ccaccatgaa aggcgcaata aattccagaa atgccagata agcagtcctg
    33841 ttaccgatgc cagcaaaaac cagggcaggt aaccaaaaaa tgcacccagg atgaaagccg
    33901 ggaggcagca aagtagcagc tccagcacca gccttttcca cgacagccgt tccagcacgt
    33961 aagatactcc agttaagaaa tcataagccc tgctctgcgt ccgatgagca aggcgttaaa
    34021 agcgggttga aaaacgatat cctgtaccgc gcacggtctg caccatgcgg tcatgcccgc
    34081 cgggctccag tgctttacgc aggcgacgaa tgtggacatc gaccgtgcgg tcttccacat
    34141 acacgttagt tccccagacg tggtttaaca gctgctcgcg gctgtacacg cgctcaggat
    34201 gcgtcataaa aaagtgcagc agtttaaatt ctgtcggccc catctccagc ggctcttcgc
    34261 ccgccatcac tcggtgagat gtcgggtcga gacttaatcc ctgcatctca atcacctctt
    34321 ccaccgccat tggcgaaata cggcgcatta ccgctttgat tcgcgccacc agctccttcg
    34381 gcgaaaacgg cttggtgata tagtcatccg cgccggtttc aaggccgcgc acgcgatctt
    34441 cttcttcccc tctggcggtc aacatcacca ctggaatatc ccgggtcatc gactcgcgct
    34501 tgaggtgttt gatgaactgg ataccggagc cgccaggtaa catccagtca aggagaatta
    34561 aatccggcca gggttcattc agttgattca cagcactgtc ataatcttcc gcttcgaccg
    34621 gctgaaagcc attttgttcg agcacgaagc agaccatttc gcgaattgga gcttcatctt
    34681 ctacgaccag aatacgtctc gccatgattt gccctgttgt aataaatagg ttgcgatcat
    34741 taatgcgacg tcattatgcg tcagatttat gacagattta tgaaaagctc gtcgcacata
    34801 tcttcaggtt attgatttcc gtggcgcaga aaaaagcaaa tggcacatct gtttgggtat
    34861 aatcgcgccc atgctttttc gccagggaac cgttatgcgc atccttcaca cctcagactg
    34921 gcatctcggc cagaacttct acagtaaaag ccgcgaagct gaacatcagg cttttcttga
    34981 ctggctgctg gagacagcac aaacccatca ggtggatgcg attattgttg ccggtgatgt
    35041 tttcgatacc ggctcgccgc ccagttacgc ccgcacgtta tacaaccgtt ttgttgtcaa
    35101 tttacagcaa actggctgtc atctggtggt actggcagga aaccatgact cggtcgccac
    35161 gctgaatgaa tcgcgcgata tcatggcgtt cctcaatact accgtggtcg ccagcgccgg
    35221 acatgcgccg caaatcttgc ctcgtcgcga cgggacgcca ggcgcagtgc tgtgccccat
    35281 tccgttttta cgtccgcgtg acattattac cagccaggcg gggcttaacg gtattgaaaa
    35341 acagcagcat ttactggcag cgattaccga ttattaccaa caacactatg ccgatgcctg
    35401 caaactgcgc ggcgatcagc ctctgcccat catcgccacg ggacatttaa cgaccgtggg
    35461 ggccagtaaa agtgacgccg tgcgtgacat ttatattggc acgctggacg cgtttccggc
    35521 acaaaacttt ccaccagccg actacatcgc gctcgggcat attcaccgcg cacagattat
    35581 tggcggcatg gaacatgttc gctattgcgg ctcccccatt ccgctgagtt ttgatgaatg
    35641 cggtaagagt aaatatgtcc atctggtgac attttcaaac ggcaaattag agagcgtaga
    35701 aaacctgaac gtaccggtaa cgcaacccat ggcagtgctg aaaggcgatc tggcgtcgat
    35761 taccgcacag ctggaacagt ggcgcgatgt atcgcaggag ccacctgtct ggctggatat
    35821 cgaaatcact actgatgagt atctgcatga tattcagcgc aaaatccagg cattaaccga
    35881 atcattgcct gtcgaagtat tgctggtacg tcggagtcgt gaacagcgcg agcgtgtgtt
    35941 agccagccaa cagcgtgaaa ccctcagcga actcagcgtc gaagaggtgt tcaatcgccg
    36001 tctggcactg gaagaactgg atgaatcgca gcagcaacgt ctgcagcatc ttttcaccac
    36061 gacgttgcat accctcgccg gagaacacga agcatgaaaa ttctcagcct gcgcctgaaa
    36121 aacctgaact cattaaaagg cgaatggaag attgatttca cccgcgagcc gttcgccagc
    36181 aacgggctgt ttgctattac cggcccaaca ggtgcgggga aaaccaccct gctggacgcc
    36241 atttgtctgg cgctgtatca cgaaactccg cgtctctcta acgtttcaca atcgcaaaat
    36301 gatctcatga cccgcgatac cgccgaatgt ctggcggagg tggagtttga agtgaaaggt
    36361 gaagcgtacc gtgcattctg gagccagaat cgggcgcgta accaacccga cggtaatttg
    36421 caggtgccac gcgtagagct ggcgcgctgc gccgacggca aaattctcgc cgacaaagtg
    36481 aaagataagc tggaactgac agcgacgtta accgggctgg attacgggcg cttcacccgt
    36541 tcgatgctgc tttcgcaggg gcaatttgct gccttcctga atgccaaacc caaagaacgc
    36601 gcggaattgc tcgaggagtt aacaggcact gaaatctacg ggcaaatctc ggcgatggtt
    36661 tttgagcagc acaaatcggc ccgcacagag ctggagaagc tgcaagcgca ggccagcggc
    36721 gtcgcgttgc tcacgccgga acaagtgcaa tcgctgacag cgagtttgca ggtacttact
    36781 gacgaagaaa aacagttact taccgcgcag cagcaagaac aacaatcact aaactggtta
    36841 acgcgtctgg acgaattgca gcaagaagcc agccgccgtc agcaggcctt gcaacaggcg
    36901 ttagccgaag aagaaaaagc gcaacctcaa ctggcggcgc tcagtctggc acaaccggca
    36961 cgaaatcttc gtccgcactg ggaacgcatc gcagaacaca gcgcggcgct ggcgcatact
    37021 cgccagcaga ttgaagaagt aaatactcgc ttacagagca caatggcgct tcgcgcgagc
    37081 attcgccacc acgcggcgaa gcagtcagca gaattacagc agcagcaaca aagcctgaat
    37141 acctggttac aggaacacga ccgcttccgt cagtggaaca acgaactggc gggttggcgt
    37201 gcgcagttct cccaacaaac cagcgatcgc gagcatctgc ggcaatggca gcaacagtta
    37261 acccatgctg agcaaaaact taatgcgctt gcggcgatca cgttgacgtt aaccgccgat
    37321 gaagttgcta ccgccctggc gcaacatgct gagcaacgcc cactgcgtca gcacctggtc
    37381 gcgctacatg gacagattgt tccccaacaa aaacgtctgg cgcagttaca ggtcgctatc
    37441 cagaatgtca cgcaagaaca gacgcaacgt aacgccgcac ttaacgaaat gcgccagcgt
    37501 tataaagaaa agacgcagca acttgccgat gtgaaaacca tttgcgagca ggaagcgcgc
    37561 atcaaaacgc tggaagctca acgtgcacag ttacaggcgg gtcagccttg tccactttgt
    37621 ggttccacca gccacccggc ggtcgaggcg tatcaggcgc tggagcctgg tgttaatcag
    37681 tctcgattac tggcgctgga aaacgaagtt aaaaagctcg gcgaagaagg tgcggcgctg
    37741 cgcgggcaac tggacgcctt aacaaagcag cttcagcgcg atgaaaacga agcgcaaagc
    37801 ctccgacaag atgagcaagc acttactcaa caatggcaag ccgtcacggc cagcctcaat
    37861 atcaccttgc agccacagga cgatattcaa ccgtggctgg atgcacaaga tgagcacgaa
    37921 cgccagctgc ggttactcag ccaacgacat gaattacaag ggcagattgc cgcgcataat
    37981 cagcaaatta tccagtatca acagcaaatt gaacaacgcc agcagcagct cttaacggca
    38041 ttggcgggtt atgcactgac attgccacag gaagatgaag aagagagctg gttggcgaca
    38101 cgtcagcaag aagcgcagag ctggcagcaa cgccagaacg aattaaccgc gctgcaaaac
    38161 cgtattcagc agctgacgcc gattctggaa acgttgccgc aaagtgatga tctcccgcac
    38221 agcgaagaaa ctgtggcgct ggataactgg cggcaggttc atgaacaatg tctcgcatta
    38281 cacagccagc agcagacgtt acagcaacag gatgttctgg cggcgcaaag tctgcaaaaa
    38341 gcccaggcgc agtttgacac cgcgctacag gccagcgtct ttgacgatca gcaggcgttc
    38401 cttgcggcgc taatggatga acaaacacta acgcagctgg aacagctcaa gcagaatctg
    38461 gaaaaccagc gccgtcaggc gcaaacgctg gtcactcaga cagcagaaac gctggcacag
    38521 catcaacaac accgacctga cgggttggct ctcactgtga cggtggagca gattcagcaa
    38581 gagttagcgc aaactcacca aaagttgcgt gaaaacacca cgagtcaagg cgagattcgc
    38641 cagcagctga agcaggatgc agataaccgt cagcaacaac aaaccttaat gcagcaaatt
    38701 gctcaaatga cgcagcaggt tgaggactgg ggatatctga attcgctaat aggttccaaa
    38761 gagggcgata aattccgcaa gtttgcccag gggctgacgc tggataattt agtccatctc
    38821 gctaatcagc aacttacccg gctgcacggg cgctatctgt tacagcgcaa agccagcgag
    38881 gcgctggaag tcgaggttgt tgatacctgg caggcagatg cggtacgcga tacccgtacc
    38941 ctttccggcg gcgaaagttt cctcgttagt ctggcgctgg cgctggcgct ttcggatctg
    39001 gtcagccata aaacacgtat tgactcgctg ttccttgatg aaggttttgg cacgctggat
    39061 agcgaaacgc tggataccgc ccttgatgcg ctggatgccc tgaacgccag tggcaaaacc
    39121 atcggtgtga ttagccacgt agaagcgatg aaagagcgta ttccggtgca gatcaaagtg
    39181 aaaaagatca acggcctggg ctacagcaaa ctggaaagta cgtttgcagt gaaataacta
    39241 ttcagcagga taatgaatac agaggggcga attatctctt ggccttgctg gtcgttatcc
    39301 tgcaagctat cactttattg gctacggtga ttggtagccg ttctggtggt tgtgatggtg
    39361 gtatgaaaaa agtcatttta tctttggctc tgggcacgtt tggtttgggg atggccgaat
    39421 ttggcattat gggcgtgctc acggagctgg cgcataacgt aggaatttcg attcctgccg
    39481 ccgggcatat gatctcgtat tatgcactgg gggtggtggt cggtgcgcca atcatcgcac
    39541 tcttttccag ccgctactca ctcaaacata tcttgttgtt tctggtggcg ttgtgcgtca
    39601 ttggcaacgc catgttcacg ctctcttcgt cttacctgat gctcgccatt ggtcggctgg
    39661 tatccggctt tccgcatggc gcattttttg gcgtcggagc gatcgtgtta tcaaaaatta
    39721 tcaaacccgg aaaagtcacc gccgccgtgg cggggatggt ttccgggatg acagtcgcca
    39781 atttgctggg cattccgctg ggaacgtatt taagtcagga atttagctgg cgttacacct
    39841 ttttattgat cgctgttttt aatattgcgg tgatggcatc ggtctatttt tgggtgccag
    39901 atattcgcga cgaggcgaaa ggaaatctgc gcgaacaatt tcactttttg cgcagcccgg
    39961 ccccgtggtt aattttcgcc gccacgatgt ttggcaacgc aggtgtgttt gcctggttca
    40021 gctacgtaaa gccatacatg atgtttattt ccggtttttc ggaaacggcg atgaccttta
    40081 ttatgatgtt agttgggcta gggatggtgc tgggaaatat gctaagtggc aggatttcag
    40141 gacgttattc accactgcgc attgcagcag tgactgactt tataattgta ctggcactgc
    40201 tgatgctctt tttctgcggc ggcatgaaaa caacgtcgct tatttttgct tttatttgtt
    40261 gcgcgggatt atttgccctt tcagcaccgc tacaaatatt gttactacaa aacgccaaag
    40321 gcggagagtt attaggtgcc gcaggtgggc aaatagcgtt taacctcggt agcgccgtcg
    40381 gcgcatattg cggaggtatg atgctgacgc tggggctggc atataattac gtggcgctgc
    40441 ctgccgccct gctttcgttt gctgcgatgt cgtcgttgct gctgtatggt cgctataagc
    40501 gccagcaagc ggcggatact ccggtgctgg cgaaaccact ggggtagatt actgtcacgg
    40561 tgcgcgttgc ttattgccgg atgcggcgtg aacgccttat ccgccctacg cggttctggc
    40621 acattttgta ggcctgataa gacgcgacaa gcgtcgcatc aggcatcgga gcacttattg
    40681 ccggatgcgg cgtgaacgcc ttatccggcc tacggttctg gcaccttttg taggcctgat
    40741 aagacgcggc aagcgtcgca tcaggcatga tgcgccaatt gcctacgttt tttactcttg
    40801 tggccataac cacgcagcgc cgcgtacgcc gctggaatca ccgtgcttcg ccttacgcac
    40861 cggcgtttca cattcgccgc cgaagacaaa ttgtttaatc aactgcccaa ccgtttgata
    40921 taaacggtct acattgctca tcccgccccc caggacaatc acatccggat cgagaatatt
    40981 cacgacatgt gccagcgatt ttgccagccg cagctcgtag cgacgcaatg ccagttccgc
    41041 taccggatcg ctttcttcaa ccaggcggat aatttcactg cctttcagcg catgtccgct
    41101 caaacgacga taatccatcg cgaatcccgt gcccgaaata aaggtttcaa tacaaccttg
    41161 tttaccgcaa taacaaggga cttcctcgcg ataacgcagt tcgtcttcgt ccatccacgg
    41221 tagcggattg tgtccccact cacctgccgt gccattgccg ccgatatgcg cccgcccatt
    41281 gaatgccacg cccgcgccgc atcccgtgcc gataatcacg gcaaataccg tctgcgctcc
    41341 cgctgccgcg ccatctactg cttctgaaac cgccagacag ttagcgtcat ttgccagccg
    41401 cacttcccgc tgcaacctcg cgcttaagtc tttatcgaat ggctgaccgt tgagccaggt
    41461 tgaattggca ttcttcacca caccggtgta aggcgaaatt gagccaggaa tgcccatacc
    41521 taccgttccg cgctgccccg tcgcctgctc cgccatatca accaacgtgg cgatcgtttc
    41581 aatagtctgc cggtaatcat cacgcggcgt gggcagacga tggcggtaca actgctcccc
    41641 tgcatcgccc agtgcaatca cttcagtttt ggtgccgcct aaatcgatac ctatacgcac
    41701 ggtactctcc ttattttttt caatatcaat agcgtagaga cggacaaccg gattggcaat
    41761 gcaaggccgc cgacaattcg ttatcatgcc cgctaaattt aacgacaagg ccgtggaaat
    41821 tatcatgctg tggttcaaaa atttaatggt ttaccgtctt agccgcgaga tttcgctgcg
    41881 tgcagaagag atggaaaaac agctagcctc gatggcattt accccatgcg gcagccagga
    41941 catggcgaag atgggctggg ttcctccgat gggatcgcac agcgatgcgt taacgcacgt
    42001 tgccaatggt caaattgtta tctgcgcgcg caaagaagaa aaaatcctcc cgtctccggt
    42061 gattaaacag gcgctggaag cgaaaatcgc caaactggaa gcggaacagg cgcgtaagct
    42121 gaagaaaacc gaaaaagatt cgctgaaaga cgaagtgctg cactctctgc tgccgcgtgc
    42181 tttcagccgt tttagccaga caatgatgtg gatcgacacg gttaacggtt tgattatggt
    42241 ggactgcgcc agtgccaaaa aagcggaaga tacgctggca ttactgcgta aaagcctggg
    42301 gtcgttaccg gttgtaccat tgagcatgga aaacccgatt gaactgacgc tgaccgaatg
    42361 ggttcgctcc ggtagtgcgg cacagggctt ccagctgctt gatgaagccg agctgaaatc
    42421 gttgctggaa gatggcggcg tgatccgcgc gaagaaacaa gatctgacca gcgaagagat
    42481 caccaatcac attgaagccg gaaaagtggt gactaaactg gcgctcgact ggcagcagcg
    42541 cattcagttt gtgatgtgcg acgatggttc gctcaagcgt ctgaagttct gcgacgagct
    42601 gcgcgatcaa aacgaagata tcgaccgtga agatttcgcc cagcgttttg atgccgattt
    42661 catcctgatg actggtgaac tggcagcgtt aattcaaaac ctgattgaag gattaggtgg
    42721 cgaagcacaa cgttaattgc tgattttcct ttaatgccgg atgcgacgcc tgccgcgtct
    42781 tatccggcgt acgaagccac accaggcata taattattcg ctacggcgag caataatttt
    42841 tagcgcagca atattatgcg ttttacgctg taacttgctc catgaacgtt gtgtcattgt
    42901 ttttcctcaa gccggtgacg cgcttcactg cgaattttcc gcgttgcccc ggttttcact
    42961 aaatcacgta acagcgaaag atcgggttgt tcgacaaata agagtaatga tggatcctct
    43021 tttaaccacg ccgttttcac atcggcagcc agctgtggat tattaatagc cagcgaccga
    43081 tcctgaggct cagtcaatgt cgttaacaac aacgccgggg tatgtgcgtt gcgcaatgcc
    43141 gtttggcgta aatgatcatt gcgactttgc agcaatgcac gccaccattc atcatctgat
    43201 ttatctttta tcgtagccag ttccgcaaca atatctggag atcttcactg atatttatat
    43261 caaccttgat aaattatgaa tatatcggaa
//
LOCUS       DAPHBJ010000013        42749 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.013, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000013 DAPHBJ010000000
VERSION     DAPHBJ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.013"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            315..1181
                     /gene="folD"
                     /locus_tag="RHZ76_000690"
     CDS             315..1181
                     /gene="folD"
                     /locus_tag="RHZ76_000690"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDV2811382.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1183..1395
                     /gene="ybcJ"
                     /locus_tag="RHZ76_000691"
     CDS             1183..1395
                     /gene="ybcJ"
                     /locus_tag="RHZ76_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HDV2811383.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAISEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1503..2024
                     /locus_tag="RHZ76_000692"
     CDS             1503..2024
                     /locus_tag="RHZ76_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV2811384.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVFIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(2060..3445)
                     /gene="cysS"
                     /locus_tag="RHZ76_000693"
     CDS             complement(2060..3445)
                     /gene="cysS"
                     /locus_tag="RHZ76_000693"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDV2811385.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3619..4113
                     /gene="ppiB"
                     /locus_tag="RHZ76_000694"
     CDS             3619..4113
                     /gene="ppiB"
                     /locus_tag="RHZ76_000694"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HDV2811386.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4116..4838
                     /gene="lpxH"
                     /locus_tag="RHZ76_000695"
     CDS             4116..4838
                     /gene="lpxH"
                     /locus_tag="RHZ76_000695"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HDV2811387.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRKMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4956..5465
                     /gene="purE"
                     /locus_tag="RHZ76_000696"
     CDS             4956..5465
                     /gene="purE"
                     /locus_tag="RHZ76_000696"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDV2811388.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5462..6529
                     /gene="purK"
                     /locus_tag="RHZ76_000697"
     CDS             5462..6529
                     /gene="purK"
                     /locus_tag="RHZ76_000697"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDV2811389.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGAHGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLSATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(6668..7561)
                     /locus_tag="RHZ76_000698"
     CDS             complement(6668..7561)
                     /locus_tag="RHZ76_000698"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDV2811390.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7558..8373)
                     /locus_tag="RHZ76_000699"
     CDS             complement(7558..8373)
                     /locus_tag="RHZ76_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDV2811391.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRQFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8384..9643)
                     /locus_tag="RHZ76_000700"
     CDS             complement(8384..9643)
                     /locus_tag="RHZ76_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDV2811392.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWVKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACPGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9653..11320)
                     /locus_tag="RHZ76_000701"
     CDS             complement(9653..11320)
                     /locus_tag="RHZ76_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDV2811393.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGAGSSQAL
                     TQVRRWDSACQKLPDASLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIRPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            11637..12686
                     /gene="allD"
                     /locus_tag="RHZ76_000702"
     CDS             11637..12686
                     /gene="allD"
                     /locus_tag="RHZ76_000702"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HDV2811394.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHLVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            12708..13943
                     /gene="allC"
                     /locus_tag="RHZ76_000703"
     CDS             12708..13943
                     /gene="allC"
                     /locus_tag="RHZ76_000703"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HDV2811395.1"
                     /translation="MITHFRQAIEEALPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLNGTEYPQEVILSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPDAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKDL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            13954..14739
                     /gene="allE"
                     /locus_tag="RHZ76_000704"
     CDS             13954..14739
                     /gene="allE"
                     /locus_tag="RHZ76_000704"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HDV2811396.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVITGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(14967..16112)
                     /gene="glxK"
                     /locus_tag="RHZ76_000705"
     CDS             complement(14967..16112)
                     /gene="glxK"
                     /locus_tag="RHZ76_000705"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HDV2811397.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDAHYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGETVNAFYSLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGEINLFNSARNIACAIKIGQGIKN"
     gene            complement(16134..17435)
                     /locus_tag="RHZ76_000706"
     CDS             complement(16134..17435)
                     /locus_tag="RHZ76_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HDV2811398.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(17492..18853)
                     /gene="allB"
                     /locus_tag="RHZ76_000707"
     CDS             complement(17492..18853)
                     /gene="allB"
                     /locus_tag="RHZ76_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HDV2811399.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKSEGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHICHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(18913..20367)
                     /locus_tag="RHZ76_000708"
     CDS             complement(18913..20367)
                     /locus_tag="RHZ76_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HDV2811400.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFRGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLIFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPSG
                     IQKAENGGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIHFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(20536..21414)
                     /gene="glxR"
                     /locus_tag="RHZ76_000709"
     CDS             complement(20536..21414)
                     /gene="glxR"
                     /locus_tag="RHZ76_000709"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDV2811401.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(21514..22290)
                     /gene="hyi"
                     /locus_tag="RHZ76_000710"
     CDS             complement(21514..22290)
                     /gene="hyi"
                     /locus_tag="RHZ76_000710"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HDV2811402.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKHVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(22303..24084)
                     /gene="gcl"
                     /locus_tag="RHZ76_000711"
     CDS             complement(22303..24084)
                     /gene="gcl"
                     /locus_tag="RHZ76_000711"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HDV2811403.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(24174..24989)
                     /gene="allR"
                     /locus_tag="RHZ76_000712"
     CDS             complement(24174..24989)
                     /gene="allR"
                     /locus_tag="RHZ76_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HDV2811404.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(25067..25549)
                     /gene="allA"
                     /locus_tag="RHZ76_000713"
     CDS             complement(25067..25549)
                     /gene="allA"
                     /locus_tag="RHZ76_000713"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HDV2811405.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            25779..26705
                     /gene="allS"
                     /locus_tag="RHZ76_000714"
     CDS             25779..26705
                     /gene="allS"
                     /locus_tag="RHZ76_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HDV2811406.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            26774..27868
                     /gene="mnmH"
                     /locus_tag="RHZ76_000715"
     CDS             26774..27868
                     /gene="mnmH"
                     /locus_tag="RHZ76_000715"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDV2811407.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQAIIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            complement(28581..30995)
                     /locus_tag="RHZ76_000716"
     CDS             complement(28581..30995)
                     /locus_tag="RHZ76_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2811408.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(30992..31678)
                     /locus_tag="RHZ76_000717"
     CDS             complement(30992..31678)
                     /locus_tag="RHZ76_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2811409.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            31649..32272
                     /gene="tesA"
                     /locus_tag="RHZ76_000718"
     CDS             31649..32272
                     /gene="tesA"
                     /locus_tag="RHZ76_000718"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HDV2811410.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            32262..33071
                     /gene="ybbO"
                     /locus_tag="RHZ76_000719"
     CDS             32262..33071
                     /gene="ybbO"
                     /locus_tag="RHZ76_000719"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDV2811411.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            33132..33986
                     /gene="cnoX"
                     /locus_tag="RHZ76_000720"
     CDS             33132..33986
                     /gene="cnoX"
                     /locus_tag="RHZ76_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HDV2811412.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEEI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(34049..34828)
                     /gene="fetB"
                     /locus_tag="RHZ76_000721"
     CDS             complement(34049..34828)
                     /gene="fetB"
                     /locus_tag="RHZ76_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HDV2811413.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(34815..35492)
                     /gene="fetA"
                     /locus_tag="RHZ76_000722"
     CDS             complement(34815..35492)
                     /gene="fetA"
                     /locus_tag="RHZ76_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HDV2811414.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKDIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            35638..36555
                     /locus_tag="RHZ76_000723"
     CDS             35638..36555
                     /locus_tag="RHZ76_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDV2811415.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            36552..37010
                     /locus_tag="RHZ76_000724"
     CDS             36552..37010
                     /locus_tag="RHZ76_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDV2811416.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(37011..37418)
                     /gene="cueR"
                     /locus_tag="RHZ76_000725"
     CDS             complement(37011..37418)
                     /gene="cueR"
                     /locus_tag="RHZ76_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HDV2811417.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(37543..38835)
                     /locus_tag="RHZ76_000726"
     CDS             complement(37543..38835)
                     /locus_tag="RHZ76_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV2811418.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            complement(38838..39770)
                     /gene="glsA"
                     /locus_tag="RHZ76_000727"
     CDS             complement(38838..39770)
                     /gene="glsA"
                     /locus_tag="RHZ76_000727"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDV2811419.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVANQLGYNVFKG"
     gene            40032..42536
                     /gene="copA"
                     /locus_tag="RHZ76_000728"
     CDS             40032..42536
                     /gene="copA"
                     /locus_tag="RHZ76_000728"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HDV2811420.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTAEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKHLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLHNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
BASE COUNT        10438 a        11619 c        10927 g         9765 t
ORIGIN      
        1 gcgcaaaaca tattcaacac aaatatagac ctgaagcggt aaattaccag gctgaaaatt
       61 ctttttatat tgtcaggtat ttcttaaatt atcttaatcc ttaaacaagg aaataaatca
      121 gttccagatt tacaacgcca tcatggacga aaaatgaagc tttcaatctc agcgacctca
      181 gcgacggtgc gcctcacctt cgcaagaggt cgcttcacgc gataaatctg aaacgaaacc
      241 tgacagcgcg ccccgcttct gacaaaatag gcgcatcccc ttcgacctac gtaacagatg
      301 gaatcctctc tctgatggca gcaaagatta ttgacggtaa aacgattgcg cagcaggtgc
      361 gctctgaagt tgctcaaaaa gttcaggcgc gtattgcagc cggactgcgg gcaccaggac
      421 tggccgttgt gctggtgggt agtaaccctg catcgcaaat ttatgtcgca agcaaacgca
      481 aggcttgtga agaagtcggg ttcgtctccc gctcttatga cctcccggaa accaccagcg
      541 aagcggagct gttggagctt atcgatgcgc tgaatgccga caacaccatc gatggcattc
      601 tggttcaact gccgttaccg gcgggtattg ataacgtcaa agtgctggaa cgtattcatc
      661 cggacaaaga cgtggacggt ttccatcctt ataacgtcgg tcgtctgtgc cagcgcgcgc
      721 cgcgtctgcg tccctgcacc ccgcgcggta tcgtcacgct gcttgagcgt tacaacattg
      781 ataccttcgg cctcaacgcc gtggtgattg gcgcatcgaa tatcgtcggt cgtccaatga
      841 gcatggaact gttgctcgca ggttgcacca ctaccgtgac tcaccgtttc actaaaaatc
      901 tgcgtcacca cgtagaaaat gccgatctgt tgatcgttgc cgtcggcaag ccgggcttta
      961 ttcctggtga ctggatcaaa gaaggcgcaa ttgtgattga tgtcggcatc aaccgtctgg
     1021 aaaatggcaa agttgtgggc gacgtcgtgt ttgaagacgc ggctaaacgc gcctcataca
     1081 ttacgcctgt tccgggcggc gttggcccga tgacggttgc cacgctgatt gaaaacacgc
     1141 ttcaggcgtg cgttgaatat catgatccac agggtgagta acatggcgac attttcttta
     1201 ggtaaacatc cgcacgttga gctgtgcgat ttgctgaaac tggaaggctg gagcgaaagc
     1261 ggcgcgcagg cgaaaatcgc gatttccgaa ggccaggtga aagtcgacgg tgcggttgaa
     1321 acgcgcaaac gctgcaaaat cgtcgccggt cagacagtga gttttgcagg tcacagcgta
     1381 caggttgttg cctgatccct ttcccggcct gtatcaacag gccggttctc ttttcagact
     1441 tttgccctct acgccccgtt catccatgct gatagcatcg acattttgcc gaagcgacca
     1501 tcatgccaac cgttattacg cacgccgccg ttcccctatg cattggttta gggttgggtt
     1561 cgaaagtcat ccctccgcgt ctgttatttg ccggaatcat cctggcgatg ctgcccgacg
     1621 ccgacgtatt gtcgtttaaa tttggcgttg cttacggcaa tgtttttggt catcgcgggt
     1681 ttacccattc gctggtgttt gcgtttgttg tcccgctgtt atgtgtgttt attggacgcc
     1741 gatggttcag ggctgggctg attcgctgct ggctgttttt aaccgtctcg ttgctatcgc
     1801 acagcttgct ggattcggta accactggcg gtaaaggcgt tggctggctg tggccgtggt
     1861 cagatgaacg ctttttcgct ccctggcagg tgataaaagt cgcgccgttt gcattgtctc
     1921 gttacaccac gccgtacggg catcaggtga ttatttctga attgatgtgg gtgtggttgc
     1981 cggggatgct actgatggga atgttgtggt ggcgcagacg ataaccggat gcgaaaactc
     2041 gcatccggca atagcgcaat tacttacgac gccaggtggt cccttgcggg ccatcttcca
     2101 gcacgatccc catctcgtta agacgatcac gcgccgcatc cgccgccgcc cagtctttcg
     2161 ctttacgggc atccagacgc tgttgaatta acgcttcaat ctcagccact tcgctgtcgt
     2221 ctgcctgcgc gccgctttgc aggaacgctt ccggttcttg ctccagcagg cccaatacag
     2281 cggaaagttt acgcaggtga gatgccattg cattcgccgc tgccatatct tctgctttca
     2341 gacggtttac ttcacgcgcc atatcaaaca gtacggaata ggcttccggg gtgttgaaat
     2401 cgtcgtccat cgcttcaata aagcgcgctt caaacgcttc gccaccggca ggcgcaacgg
     2461 ttttatctgt gccgcgcagc gcagtgtaga gacgctccag cgccgcacgc gcctgcttca
     2521 ggttctcttc gctgtagttc aactggctgc gatagtggcc cgacatcagg aagtaacgca
     2581 cggtttccgc gtcgtagtat ttcagcacat cgcgcacggt aaagaagtta cccagcgatt
     2641 tggacatctt ctcgcggtca accatcacca ttccggagtg catccagtag ttcacatact
     2701 gaccatcatg ggcacaggtg gactgcgcga tttcgttctc gtggtgcggg aacatcaggt
     2761 ctgaaccgcc gccgtggata tcaaagtggt tacccagctg cttgcagttc atggccgaac
     2821 attcaatgtg ccagccagga cgacccgcgc cccacggaga cggccagctc ggttcgccct
     2881 ctttcgacat cttccacaga acgaagtcca tcgggttgcg tttgtcatca accacgtcaa
     2941 cgcgcgcgcc agcctgcagc tggtcgagat cctgacgcga cagcacgcca taagttggat
     3001 cggtcgggac gtcaaacatc acgtcgccgt tgtccgccac ataagcgtga cctttggcga
     3061 tcagttgttc agtgagttca ataatttctg cgatatggtg cgtcgcgcgc ggctccatat
     3121 ccgggcgcag aatgttcaaa gcatcaaaat ctttgtgcat ttcggcgatc atgcgatcca
     3181 ccagcgccac aaagctttcg ccattttcat tggcgcgttt gatgattttg tcgtcgatat
     3241 cggtaatgtt gcgcacatac ttcagtttat agccgaggaa acgcagatag cgcgcaacca
     3301 cgtcaaaagc aacaaaggta cgcccgtgac cgatatgaca gagatcgtaa acggtgattc
     3361 cacacacgta catgccgact tccccggcgt gaataggctt aaattcctct ttttggcgtg
     3421 tcagagtatt gaagattttt agcatcgaag attccgttta gacatgtgtg ggtaattgag
     3481 ttgcgtataa tacccatatt tcccgcgcga atcagcatac attgcgtgat gatccgatcg
     3541 tgcggttatg ctataacacc accctatata tgacccgaac tgggttgaag caccaatcaa
     3601 acggaacagg atgcaaaaat ggttactttc cacaccaatc acggcgatat tgtcatcaaa
     3661 acttttgacg ataaagcacc tgaaacagtt aaaaacttcc tggactactg ccgcgaaggt
     3721 ttttacaaca acaccatttt ccaccgtgtt atcaacggct ttatgattca gggcggcggt
     3781 tttgaaccgg gcatgaaaca aaaagccacc aaagaaccga tcaaaaacga agccaacaac
     3841 ggcctgaaaa atacccgtgg tacgctggca atggcacgta ctcaggctcc gcactctgca
     3901 actgcacagt tcttcatcaa cgtggttgat aacgacttcc tgaacttctc tggcgaaagc
     3961 ctgcaaggtt ggggctactg cgtgtttgct gaagtggttg acggcatgga cgtggtagac
     4021 aaaatcaaag gtgttgcaac cggtcgtagc ggtatgcacc aggacgtgcc aaaagaagac
     4081 gttatcattg aaagcgtgac cgttagcgag taatcgtggc gacactcttt attgcagatc
     4141 ttcatctctg cgtggaagaa ccggcgatca ccgccggttt tctgcgtttt ttagcggggg
     4201 aagcccgcaa ggccgacgcg ctgtatattc ttggcgatct gtttgaagca tggattggcg
     4261 acgacgatcc caacccactc catcgcaaga tggcggcggc gatcaaagcg gtgtccgatt
     4321 ccggcgttcc ctgttatttc attcatggca accgtgattt tctgctcggc aaacgctttg
     4381 cccgtgaaag tggcatgacg ttattgccgg aagaaaaggt gctcgaactt tatggtcgcc
     4441 gggtgttgat tatgcatggc gacacgctgt gcaccgatga cgcgggttat caggcttttc
     4501 gcgccaaggt ccacaaaccc tggctgcaga cgctattcct cgccctgccg ttgtttgtgc
     4561 gcaaacgcat tgccgcgcga atgcgcgcga acagcaaaga agccaacagc agtaaatcgc
     4621 tggcgatcat ggacgttaac caaaacgcgg tggtcagtgc gatggaaaaa catcaggtgc
     4681 aatggctgat ccacgggcat acccatcgcc cggcggtgca tgaacttatc gccaatcagc
     4741 aacctgcttt tcgcgtggta ctgggtgcct ggcatacgga aggttcaatg gtgaaagtca
     4801 cggcggatga cgttgagctg attcattttc cgttttaaaa aacccgcaac tttgctgatt
     4861 tcacagccac gcaaccgttt tccttgctct ctttccgtgc tattctctgt gccctctaaa
     4921 gccgagagtt gtgcaccaca ggagttttaa gacgcatgtc ttcccgcaat aatccggcgc
     4981 gtgtcgccat cgtgatgggg tccaaaagcg actgggctac catgcagttc gccgccgaaa
     5041 tcttcgaaat cctgaatgtc ccgcaccacg ttgaagtggt ttctgctcac cgcacccccg
     5101 ataaactgtt cagcttcgcc gaaagcgccg aagagaacgg ttatcaggtg attattgcgg
     5161 gcgcaggcgg cgcagcgcat ctgccaggca tgattgccgc caaaacgctg gtgccggtgc
     5221 tgggcgtgcc agtacagagc gccgcactga gcggtgtcga tagcctctac tccatcgtac
     5281 aaatgccgcg cggcattccg gtgggtacgc tggcgattgg taaagctggc gcggcaaacg
     5341 cggcgttact ggcagcacaa attcttgcga ctcatgataa agaactgcac cagcgtctga
     5401 atgactggcg caaagcccag accgacgaag tgctggaaaa cccggacccg cgaggtgcgg
     5461 catgaaacag gtttgcgtcc tcggtaacgg gcagttaggc cgtatgctgc gtcaggcagg
     5521 cgaaccgtta ggcattgctg tctggccagt cgggctggac gctgaaccgg cggcggtgcc
     5581 ttttcaacaa agcgtgatta ccgctgagat agaacgctgg ccggaaaccg cattaacccg
     5641 cgagctggcg cgccatccgg cctttgtgaa ccgcgatgtg ttcccgatta ttgctgaccg
     5701 tctgactcag aagcagcttt tcgataagct ccacctgccg actgcaccgt ggcagttact
     5761 tgccgaacgc agcgagtggc ctgcggtgtt tgatcgttta ggtgagctgg cgattgttaa
     5821 gcgtcgcact ggtggttatg acggtcgcgg tcaatggcgt ttacgcgcaa atgaaaccga
     5881 acagttaccg gcagagtgtt acggcgaatg tattgtcgag cagggcatta acttctctgg
     5941 tgaagtgtcg ctggttggcg cgcacggctt tgatggcagc accgtgtttt atccgctgac
     6001 gcataacctg catcaggacg gtattttgcg caccagcgtc gcttttccgc aggccaacgc
     6061 acagcagcag gcgcaagccg aagagatgct gtcggcgatt atgcaggagc tgggctatgt
     6121 gggcgtgatg gcgatggagt gttttgtcac cccgcaaggt ctgctgatca acgaactggc
     6181 tccgcgtgtg cataacagcg gtcactggac acaaaacggt gccagcatca gccagtttga
     6241 gctgcatctg cgggcgatta ccgatctgcc gttaccgcaa ccagtggtga ataatccgtc
     6301 ggtgatgatc aatctgattg gtagcgatgt gaattatgac tggctgaaac tgccgctggt
     6361 gcatctgcac tggtacgaca aagaagtccg tccgggccgt aaagtggggc atctgaattt
     6421 gaccgacagc gacacatcgc gtctgtccgc gacgctggaa gccttaatcc cgctgctgcc
     6481 gccggaatat gccagcggcg tgatgtgggc acagagtaag ttcagttaac ctggtgctca
     6541 tttgattgcc ggatgcggcg tgaacgcctt atccggccta caccgatccg gtacccattg
     6601 taggcctgat aagacgcgtc aagcgtcgca tcaggcatgg tgcaccgatt gctggatgct
     6661 ctcatcacta cagcgaaata caggtccccg cttcgcccgc cagcgtctct tcaattcgcg
     6721 ataacgcccc aatccacgcg ggtttaccac ggcttctgac ataaccactc accgccgtta
     6781 ccttcggccc catcgaacca tcggctttgg caaatggcgc taactcatcc ggtgtggcat
     6841 ggcgaatggc gcgttgctgc ggcgttcccc agttttcata taccgcatca gcatcagtga
     6901 ggatcaccag cccatcggcg ttaatctgct cagcgagcaa cgcagcagcg agatctttat
     6961 caatcaccgc ttcactccct gccccatctt ccgtcacagg cacaccgccg ccgccactgc
     7021 aaatcaccac atgcccctct ttgagcaaca actcgatggc ttcgctgtcg agaattttac
     7081 gcggttgcgg agacgccacc acccggcgca aatatttacc atcacgtttc atctgccagc
     7141 cgtaagctgc ttccagtgcc tcttgttctt ctggctgata gaccggaccg ataaacttct
     7201 ctggctgcaa aaacgccgga tcatcaggcg aaacttcaat gcgcgtcagc accgtcgtga
     7261 caggcggcat ctgcggctgt gcgctcaaac tctgcgccag catatagcca atcatcccct
     7321 gactttctgc caccagcaca tccagcggat acggttccac ttctttccac gccagattct
     7381 gtaacgccag cagccccacc tgcggcccgt tgccgtgaac aatcgccagc cgataagaac
     7441 gggccaggcg cgccagcgca ggtacagcac tggcgatatt gcgatattga ttttctgccg
     7501 tcagcgcctc accgcgctga agcaaggcat tgcccccaag agctacaacc agtgttttca
     7561 tggttttcct tgtaataatt gttgaccaag ccagaacccc agcagtgtat ctgcccctga
     7621 cgtatgcccc agcgccagca gcgaatcaat cgcaacagcg gtacgtttcg ggcaactcag
     7681 agcatggaca aagtgcagga gtggcgaggc gaaatatcct tgcgcagcat aacgtaaata
     7741 actgacgctc accgctgtgg taacgagttg aagattgtcg gaacaggcaa aaaactggcg
     7801 accggagcgc gcatctaaag caccataata ccaggccgcc agcagcattc cgctcagcgt
     7861 gtcatcatga cttggcgtta atccggggcc tttacccagc cagtgccgcc agtcggtctt
     7921 aacgccattg agcgcggcct gaaaacagtg acgaaactgg cgtaactcag caggcagcgg
     7981 atcgcctgcc gccaacgcca gtggcccgaa aagcccggtt tcctccgcgc gttgcatcca
     8041 tgcagctgca agcggttgag gatgcgcagg cggcgtaata cgcagcaaac aataacgctg
     8101 tggctgttta accgtgaaac gcccgaggcg aatcccttga gccacaacct gtggtcgttc
     8161 attgccgcat aatccaccgc ataacgcatc gaattgcgcg cggcgaagca cccatcctcc
     8221 ggggccgaaa ccactaccct gacgatgcag cgttaacagt tcgccgcttt ccgtcatcag
     8281 gttaatcgcc cgccgccaca ctcccgctaa ccgccaggac tggcgataat tcggtgcgct
     8341 actactggca agcagaggat ggatgatcgt cattgcgcgt tcctcaacca atccccatgc
     8401 tttcagccag tgcttccagc gcctgttcaa aacacgccag cggtgcccgc acagtgcctg
     8461 cgccaatctg cccgatcccc gcctctttat gggcgatacc ggtattgatg agtggcgtaa
     8521 tgccggtttc taccacgcga cgaatgtcca gtcccgggca cgcgccctga aaatcccagc
     8581 ctgggatctg caactgcata ttgcgctcaa ggtaaatttc cgccatctct tcagataccg
     8641 ctctggccgc ttccatgcca cccgcaccga caaagcgcgt tacgccaggc gctgcgatca
     8701 ttgccgcacc tccgatacca aaggtttcgg taatcgcgct atcgcccata tccgggttcg
     8761 cctgctcctg cgagaagccg gtgaaaaaca gaccttgcgg agtgtttaca ggcgcagtaa
     8821 accagcgttc gcccagcccg cttacccgaa tcccgaacat attgccgttg cgggtcattg
     8881 ccgtgacgat gctgcctgcg cggatcatcg cgccagcatc catcgccgcc ttgcagtaag
     8941 ccatcgcgag gttgaggaag aactgatcgg tcacgctgag gaaatccatc acttcggcga
     9001 tgtgctgttt atcatgatcg aggcgagcaa tttgtggggc cagcgcacgc attaacagtg
     9061 cagaggaagc aatattgcgt tgatggaact catcgcccat cgtaatgccc tgcgccatca
     9121 tcgccgtgag atcgataccg cgctccatgc gccccagcgc cgcgcttaat actggcatta
     9181 acacatcgcg catccagcga tggcgagtca ggacatcttc gccgtaagcg ccaaaacgca
     9241 tcactttgcc gataccttcg ttgaggttgc agtacgcccg gttgccgtcg gtcacgttct
     9301 cgaccaccag catcggcata ctggcagaag taataccgcc cattggaccg acggcattca
     9361 catggtgaca aggaatgaag ttcacttccc cctgctccag tattgccagc gcctgcgctt
     9421 catctttcac ccagccttcg aacagacatg cgcccacgca cgcccctttc atgggtccgg
     9481 tcatctcctg ccagcgcatt ggcggcccgg cgtgaagcag tgttttgccc tcgtttagtt
     9541 cgctgataag tgaagaagcc ggttgcacat ccagccagtg tggacgagcg cgacgaattt
     9601 gttcgataac cgcagcattg gcttgcgcta ctgatgtaaa catgggaacc ccttattgca
     9661 aacgttctaa taaacgagcc agttttttat tgccgccggc gactggcgac cattggtaat
     9721 gcacaaccgg tttgctggcg ctttgtagct ccagcgcaaa gctgcgtaat ccgatgttaa
     9781 tcacggcgac gttttccagt aatgacggtg tgtgttgctg agtggcaggc gaaagcggac
     9841 gaatcagtgc cgccgccagc aaggtggctt ccggcagcga actcactacc gcgatccctg
     9901 catcttccag cgtggcgatt tgctgcgagc ggcattgcgg gtcacgttca gtgcctgtca
     9961 ccgtggcaat ggcatacagt ggttgattgt ctgaacgcgc ggcacatgct ttttgccagg
    10021 cgcttaccag cgaggcggca ggatcggcgg tcgcaccgaa gccaatcacg acatcaagca
    10081 gcaatacgcg cacttgcggt ttagcgccga gatcggcaat taactggttg cgtaaggctg
    10141 ggtcgatcat cggatgggga cgcccgacgg tgtagaaatc atcgccgagg tcaataatct
    10201 ggtgaccatc ggcgtcgagc atcatgccgt gatgatgggt atcgtcggct tccacgccaa
    10261 ggtgtccagc aagtaatccc gccgcttcgg cagccagcgt accgccggta tacaaaccgc
    10321 aaataaatcc gctgctgaca ggcgctattg cgttacgtcg cgccgtgaca cgtgaaagca
    10381 ggcaagccag gcgtgcggcc tcatccagcg aggaggcaaa ccagacattc tcgtcgcggg
    10441 ccaccgccgg ggtataacct aaaaacagcg ccaccgtcgg tttgccggtt gctttcatgg
    10501 cattaacaat tttcagacgc acagcttcgg caggcggttt tgaaacaaat gccagcactt
    10561 cgcttttctc gtctgcactg agcatttcca gcgccgtcag cgcactgatg ccgcctactt
    10621 cacggctgag gtcgcgcccg ccaaggccaa tcgcgtgagt aattccctcc cctgccagcg
    10681 caatctgcga gcacagctcc tgaatcccgg taccggaagc gccaatgacg ccaatgttgc
    10741 cttccggcat cacgttagca aaagccagcg gcgtgccggc aatcatcgat gtaccgcagt
    10801 ccggccccat caccagcaag cctttttccc gcgcgcgggt tttaagttgg atttcatctt
    10861 ccagcgtgac gttatcggag aacatcatca cgttgaggtt gcgatccagc gcctggttcg
    10921 ccagctccgc cgcatactca ccagccactg aaatcagcgc cagactggcg tcgggtaatt
    10981 tctgacaggc actgtcccag cgacgcacct gcgtcaacgc ctggctgctg cctgccccct
    11041 gcgccagttg ttttagcgcc tcttcaagct gctgcataat cgcctgcgcg atccccgcat
    11101 ccgccgcttc gctacgaatt gccacgcaaa tatcgttcgg cgtggcgtta ttaaaatcgt
    11161 catgccagaa acctgtggta tctaataacg ctttattggc gggcgtaccc atcattacgg
    11221 aaacatcatc aacattttct gattcgctga gttttcgtga aataatcatt aaactgaccg
    11281 aatcctgaaa acaccctttt ttaataaagg cgtggatcat aacaactcct taaaaattgc
    11341 acccatggca attgcacgag cagtgttaat gtggcgatta cggtatcgat gacaacgggt
    11401 aaataaggtg atgaatatca cacagggatt attaacaaaa gaattcaaat aaccgccatg
    11461 ctattacgtg ttatttttta ataagcgtgg ttgttttaaa aaccaggtta aaagcgagcg
    11521 gattatcaaa taaattctaa ctatattttt ttgcctgtct ggatcacata atccagatat
    11581 tttccctgta tgttaattgc agtcatgctt cacaccgtcg ttaaaaagga agacagatga
    11641 aaatcagtcg ggaaacactc caccagctaa ttgagaataa actctgccag gctgggttaa
    11701 aacgtgagca cgctgcaacc gtggctgaag tattggttta cgccgatgcc agagggatcc
    11761 actctcatgg cgcggtgcgc gtggaatact acgcggaacg catttcaaaa ggcggcacca
    11821 accgcgaacc ggagtttcgt cttgaggaaa ccgggccgtg ctcggcaatt ttacatgccg
    11881 acaatgccgc cggacaggtc gcggcgaaaa tgggtatgga acatgccatc aaaaccgccc
    11941 agcaaaatgg cgttgcggtg gtcggtatca gccggatggg tcacagcggc gcaatctctt
    12001 attttgtgca gcaggcagcc cgcgccggat taattggcat ttcgatgtgc cagtccgatc
    12061 caatggtggt gccgtttggc ggcgcggaaa tttactacgg tactaaccca ctggcctttg
    12121 ccgcgccggg agaaggcgac gagatcctta cctttgatat ggcgactacc gtacaggcat
    12181 ggggaaaagt gctcgacgcc cgctcgcgta atatgtctat cccggatacc tgggcggtcg
    12241 ataaaaacgg tgcaccaaca accgatccgt tcgcggtaca tgctctgctc cccgccgccg
    12301 ggccgaaagg gtatggcctg atgatgatga ttgacgtcct ctcaggcgtc ttactcggct
    12361 taccgttcgg gcgacaggtt agttcgatgt atgacgattt acacgccggg cgtaatttgg
    12421 ggcaattaca tttagttatt aacccgaact ttttctcctc cagcgaatta ttccgtcaac
    12481 atcttagcca gaccatgcgc gaattaaatg ccattacccc cgcgcccggt tttaaccaag
    12541 tttattatcc cggacaggat caggatatta aacaacgcaa agccgccgtc gaaggcatcg
    12601 aaattgttga tgatatttac cagtatttaa tttccgacgc gctttataac acgtcatacg
    12661 aaacgaaaaa tccctttgcg caataattat tgagacagga ctttcttatg attacacatt
    12721 ttcgtcaagc catagaagaa gcgctgccct ggctttcctc ttttggcgct gacccaacgg
    12781 gtgggatgac ccgtttactt tattcgccgg aatggctgga aacccagcag caatttaaaa
    12841 aaagaatggc agcaagtggg ctggaaacac gtttcgatga agtagggaat ttatacggtc
    12901 gcctgaatgg taccgaatat ccacaggaag tgattctgag cggttcgcat atcgataccg
    12961 tggttaacgg cggtaacctc gacgggcaat tcggcgcgct ggcggcgtgg ctggcaattg
    13021 actggctgaa aacgcaatac ggcgcaccgt tacgtacggt cgaagtggtg gcgatggcag
    13081 aagaagaagg cagccgcttc ccgtatgtct tctggggcag taaaaatatc tttgggctgg
    13141 cgaatcctga cgacgtgcgg aatatctgtg atgccaaagg aaatagtttt gtcgatgcga
    13201 tgaaggcttg cggatttact ctgccggacg ccccgctaac tccgcgtcag gatattaaag
    13261 cctttgtcga actgcatatc gaacagggct gtgtgctgga aagtaatggg caatcaattg
    13321 gcgtggtgaa tgcaattgtc gggcaacgtc gctatacggt gacgctgaac ggcgaatcaa
    13381 accatgcagg caccacgccg atgggttatc gtcgtgatac ggtttacgct ttcagtcgca
    13441 tttgccatca gtcgatcgaa aaagcgaaaa agatgggcga tccgctggtt ctgacctttg
    13501 ggaaagtaga gccgcgcccg aatacggtga atgtggtgcc gggtaaaacc acgttcacca
    13561 ttgattgtcg tcataccgac gccgccgtgc tgcgtgattt cacccaacag ttagaaaacg
    13621 acatgcgggc gatttgcgat gaaatggaca ttggtattga tatcgattta tggatggacg
    13681 aagaacccgt gccgatgaat aaggacctgg tcgccaccct gacagaattg tgtgaaagtg
    13741 aaaaactgaa ttaccgggtg atgcacagtg gtgccgggca cgacgcgcaa attttcgcgc
    13801 ctcgcgtgcc gacctgcatg attttcattc ccagcatcaa cgggatcagc cataacccgg
    13861 cggaacgcac caatattacc gaccttgccg aaggggtcaa aacgttggca ctcatgcttt
    13921 atcaacttgc ctggcagaaa taaggagtca taaatgggat atttaaataa cgtcaccggt
    13981 taccgcgatg atttactggc taaccgtgcg attgtgaaac acggtaattt cgcactgtta
    14041 accccggacg gtctggtaaa aaatattatt ccgggctttg aaaattgtga cgcgacaatc
    14101 ctctccacgc caaagctggg tgcctctttt gttgattatc tggtcacact gcatcaaaac
    14161 ggcggcaacc aacagggctt cggtggcgaa ggcattgaaa cgttcctgta tgtgatcact
    14221 ggaaatatca ctgccaaagc cgaaggcaaa acatttgcct taagcgaagg tggctatctt
    14281 tattgcccgc caggctcctt aatgacgttt gttaacgccc aggccgaaga cagccaaatc
    14341 tttttatata agcgccgcta tgttccggta gaaggccatg caccgtggct ggtttctggc
    14401 aatgccagcg aactggaacg cattcactat gaaggtatgg atgatgtgat cctgctggat
    14461 tttctgccca aagagttagg ttttgatatg aacatgcata tcctctcttt tgcaccaggt
    14521 gccagccacg gttatatcga aacacatgtt caggaacacg gtgcctatat tctttccggt
    14581 cagggggttt ataacctcga caataactgg atcccggtga aaaaaggcga ttacatcttt
    14641 atgggcgctt attctttaca ggctggttat ggtgtagggc gtggtgaagc gttcagctat
    14701 atttactcga aagattgcaa ccgcgacgta gagatttaag tcataccact cgcctgatta
    14761 tttttagcta tgttggattt tgccacggtt tataccgtgg ctttttttgt aacattgcct
    14821 gatgcgcttc gcttatcagg cctacatgat ctctgcaata tatagaattt gcgcgatttt
    14881 caggaaggat atagtactca tgccgcatcc ggcatgagta ctgcgctctt atacgaaata
    14941 aatagcatcc gctttaaagg caagagtcag tttttaattc cctgacctat tttaatggcg
    15001 caggcaatat ttcgcgcgct gttgaagaga ttgatttcac cgctggcgag tacttcggct
    15061 aaaggtgcca gacgtggcaa aatgctgaat accgcatcaa tgccatactg gtgcaccact
    15121 tccacgccat cacccaatac gccagcaatc ccaatcaccg gtacattaaa ctgcttcgcc
    15181 accgacgcca cacccagcgg cgctttaccg cctgccgttt gcgagtcgat gcgcccttcc
    15241 ccggtaatca ccagtgctgc gccctgcact gcctgcgcaa gattgaccgc attcaacaca
    15301 atctcaatac ctggtttaat atccgcattg agaaataccg ccgccgcaat acccataccg
    15361 cccgcagcgc cgccgcccgc catctggcag acgttgattt cagtttgctg ttgtaaaaca
    15421 cgggcgtaat tttgcagccc ctgttccagc tcttcgacca tttccggcgt tgcccctttt
    15481 tgcgggccaa acaccgccgc cgcgccgcgt gcccctacca gcgggttatc aacgtcacat
    15541 gctacttcaa tatggcaatt cgccagacgc ggatcgcatt catccatctc aatgcttgcc
    15601 acgcgcgcta aattaccgcc gttagccacc agcacctgac cgtcggcatc aaggaaacgc
    15661 acaccgagcg cctgcgccat gcccataccg ccgtcgaccg tcgcactgcc gccaatgccg
    15721 agaataatat ggcgaatgcc gttatccagc gcatgacgaa ttaactcccc cgtaccaaaa
    15781 ctggaggcca gcaacggatt acgcttttca ggcgcgacca gcatcaggcc acttgctgcc
    15841 gccatctcaa tcaccgccgt tttcccgtcg ccggtaaggc tataaaaagc attcactgtt
    15901 tcgcccatcg gcccgcagac ttcaagcgtc acgatgttgc cgcccgtcgc ggcgaccatc
    15961 gcatccaccg ttccttcgcc gccatccgct atcggcaaac agatatagtg cgcatcggga
    16021 aagagggtcg aaaacccggc tttaattgcc tggcaacatt tttctgcact taagctctct
    16081 ttaaaagagt ctggcgcaat gacaatcttc attatcaatc ctttttcaac aaattattcg
    16141 atgcgttccc acggaataag attatccata agaacagcca gtaaaatccc gaccaataag
    16201 ccgttactga gcagaggacg cagcgttaat ggaaggtctt gcagatacac aggcggtaat
    16261 gccattaaaa atatgccgac aaataacggc aatgcgagtc gataaatatt gcgagcggta
    16321 aaggttattt gctggctaaa caccagcgcg gaaaagagta aaggcagata agaaaccagc
    16381 atgaccgcgc tactcacggg taaagggata ctgcaaaaca gtcgtgtgag tgccggaacc
    16441 agcgccacca gcaggcaaat aacgctgcca taaataaacg aacgccgcgt gtaatcgcca
    16501 gtctgggtta ataaaccaat ggatgaaaca aacggtgaaa atggaattac cgccagcggt
    16561 acggttatca gcgtcataaa tccggtcgcc acaaagctac gacgataacg cgtattcccc
    16621 gcgccctgct gcggataaaa aacatccgtg ccccgaatcg caccgtaggt attgctgata
    16681 ttcaccagac ctgtaatcac cgccgtcaga ataattcccg gcgacaaagc accgccactg
    16741 ccgagcggga accactgcca gtgcaactca ccggagagcg aatgcgaaga aggaaagcaa
    16801 aagtaccaca acaaccagcc ggttatcgta ccgaccagca ggccataacg ggcaaaacgt
    16861 tgcggcagaa agataatcat cgccagtacc aggcacatca ccgccaccga gagcgcgaac
    16921 ggtggtaact gaattttaaa atttgggtcg gctatgccaa acggcagccc gagcatacct
    16981 ttgaaaaaga tagtggtcag ctgcgcgccc agcatcaaca taaacaagac catcaccgac
    17041 ggcgtaaata accgtgccag gcgatggcct aatccgctaa aaccaatcaa catcgtcagc
    17101 acgccggaga gtgcaatccc caccgccaga ctggtagcga tatcgttgat cggtgtcccg
    17161 cgcgatgctt cgccaagggt gatagtaagg atggttcccc accacaggcc acccggccct
    17221 tccataatag cgcgacgatg accgcagaac gcctgagcga agcaggccag tgcggtagca
    17281 agaaaagcat attgcgtgag cgtaagcagg ctactttgcg gcaactgaaa agcagaaagt
    17341 agcgtaggag gaaccacaac cgtgttgcaa aaaataaaga aaaaccactg aaatcctgat
    17401 aacaggcttt cgcggctgac tgcaaaattg aacataaaca caccttaacc ggagaatgcc
    17461 cgccgcaagg acgggcattg caggggccag attactgctg atgtttaagg ataaattgac
    17521 ctttcggcgc aacagggaag ccctgttcaa tgtcgtaaat cacatcacca cgtaagatgg
    17581 ttttcgtgat acgcgcgcca atggtacggc caacatacgg gctgactttg tggcgatatt
    17641 ccagatcgtc attggtaaga acatagctgc tattcggctg aatgaagacg aagtcggcat
    17701 cttttcctgg ggcgatacgg cctttttgct gcagaccgaa aatatctgct gcgttagtcg
    17761 ccattaattt gccgaacatt ggcagagaca ttccgcgttt ctgtaccgct tcatcgaaca
    17821 tcacgtccat acagttttgc agaccggcaa taccgcccca tgcttccatg atgttgccgg
    17881 ctttcatttc cggagggcat ggtgagtggt cggaaaccag gcagtcgatt tcaccgttaa
    17941 acagtttttc ccacatgcct ttctggtttt ccagatcgcg gatcggcggt gaacacttcg
    18001 ccagagtacc gatttcttcg aactgatcgg tatccagtac aaagtaatgc gggcaggatt
    18061 cacaagtaac atcctgacct tcctgacgtg cacgagtcac ttcttcaaca ccttctgggc
    18121 tgctgatatg gcaaatgtgc agacggcaac cggcaacttt cgccaggtac agtacgcggc
    18181 gaatcgcttc cacttcggta aataccggac gcgaagccac atagtcatgg gcagttacgc
    18241 gaccttcact tttcgcttct tcgcccagtg catcacagat cagcgcgttt tcgcagtgca
    18301 ccagcaccgg ctgccccagt tcgcccagct tctgcgcacc tttgaagaac tgccagtcgt
    18361 ttacatcacg gaagtcgttg tcgataccgc gatcgccaca ggtcgcaacg aagcatttga
    18421 agccgacaac gcccacttca tccaactcat gcagacgatc aatgttgtaa gacaccaggc
    18481 caccgagttg tgccgcatcg atagtcagct tgcctttagc ggcatcgaac ttcagttcaa
    18541 ttgaagcacg gtcaaccgtt gcaggcagct ggttgagcgg catttcgatc atggtggtga
    18601 taccaccttt tgccgctgcg cgagtaccgg tttcataacc ttcccagtgg ctacgacccg
    18661 gttcagaaat atgggtgtgc gcatcaacca tgcccggcga aaccaccaga ccagacgcat
    18721 ccataacttc ttttgcatcg cccagatcct gaccgatagc agcaattttt ccgcctttaa
    18781 cggcgatatc tacaacgcga gcttcgtttt ctaaaataac ggtgccgttt ttaatgatta
    18841 aatcaaaaga cataacaaac tccttgtaaa atacagattg gtttttaact caatgttcaa
    18901 tatcgggatt aattaaccta tggttttttg ctctcctgtt ttttctgctg ttgtacgttt
    18961 ctttaataag gcgtaggccg caaaggcgac gatgacgccg acaaaccatg aaacacgcga
    19021 taacggttcc ataaagtgaa taaacttacc gccaagagat aaaataacgg ccaccagagt
    19081 tactgaaaac gcagtgaggt taaaaccgtt atcgtaatat ttataatcgc caggtgcggt
    19141 atacagttca tcaagattaa tttgtccgcg catcaccaca aaataatgcg ccatcatgac
    19201 accaattacc ggaccaagca ttccgccgat aatatcgagg aaaagataaa tgctgtcctg
    19261 attttccatt aatttccacg ggcagatcag caagctgata atactggcaa tcagtacgcc
    19321 gtttttatag gtcagttttg tcggtgcaat ggcggcaatc tgataaccgg ctggaataat
    19381 attaccggtc gcgttagtgg agatagttgt catcagaata accagtaccg caaagaacga
    19441 ggcgaacagg ctgtcccaac gctgaacaat atccagcacg ttccaggtat cagcgccgta
    19501 gtgaatactg gctccggcaa taatacatac cccggcgacc gcaaacagaa tataggccac
    19561 aactaaaccc agcgtttgcc ccagcgcctg ctcacgaaac gagtgggcgt tttgcgtaaa
    19621 gtcggatgcg ctcaccgccg gtgccgccca gaccgcaact accgcgttaa tcaccaccag
    19681 gaacaggaag ccaccgtttt ctgctttctg aataccgctc ggaatgtagt caaagattgg
    19741 accgatcccg accagtgaaa tcgcccaaat cgccataccg ccgaaaacga tatagatgca
    19801 cgggttaaga atggcagtga atttatttaa aactttgcca ccgccaaaac ctataccaac
    19861 gttgaccagc cagaagatta agaaagtaat taagcccggt agagaaaggc ctaacagagt
    19921 gaaatcacca ccgagagtta aaaatcccgg ccagattttg ccaatcagaa tcaagcaggc
    19981 cagtgacccc gcgtaacatt gcaaaccaaa ccacatgatg gcggcaattc cgccccttaa
    20041 taatccgcga aacagtgcac cacgtacacc gtaagaagca cgcaggatca tggcaaaagg
    20101 cacaccgtat ttactgcccg cagcaccgtt taataccatt accgcggcaa tgaaaaaggc
    20161 gctgaggata attgccagca taatactaaa ggtagacaag ccgagaataa aaaagccgcc
    20221 gaccatcaca taattgggaa cgttatgaac cgaacccatc cataaggtaa aatagttaaa
    20281 tgttttccag gtccgctggc tttgcgtttt cggcaataga tcttcgctat agccgcgttg
    20341 ctggaatagt tttctctgat gttccataaa tccctcttaa tttcacgtat cgaataacgt
    20401 tgttttaatc tagctatgag gaacaaataa aataaaaata ttatttttaa tactgaagca
    20461 acatctttgg ctgctttctt ttttaatatt gctggccgat tcattttctg atatcggcca
    20521 tttttattgc gggtatcagg ccagtttatg gttagccatt aattccagcg cctgcactaa
    20581 cgcagagtga tccaactggc tgccaccgtt tgccgcacag gtattaaata actcctggca
    20641 ggtcgcagtg tttggcaggt tcagcgcaag tgctttcgca ctttgcagtg ccaggttgag
    20701 atctttctgg tgcagagcga ttttgaagcc cggattaaag gtgcgtttaa tcatacgctc
    20761 gccatgaact tccagaatac gtgaggaagc aaagccgccc atcagcgcct ggcgcacacg
    20821 taccgggtcc gcaccggctt ttgaagcaaa tagcagggct tcagaaaccg cttcaatatt
    20881 gagcgccacg ataatctgat ttgccacttt gcaggtttga ccatcgccgt taccgcccac
    20941 gagggtgata tttttaccga gcagttcaaa cagcggttta acacgttcaa ataccgcttc
    21001 atcaccgcca accataatcg acaacgtccc ttcacgcgca ccgatttcac cgccggagac
    21061 tggcgcatcg agataatcgc cgcccagttc attcacctga cgagcgaaac gcttagtttc
    21121 aatcggggaa atggagctca tatcaacaat ggttttgccc ttcagcgagg ctttggtaca
    21181 accattttca ccgaacagaa cttcttcaac ctgaggtgtg tccggcacca taataaaaat
    21241 gatgtccgat gcttccgtta cctggcgagc agtttcaaca ctgacggcac ccagtgacag
    21301 taattcatca gcaaccggtc caatggtcgt gacatgtaat tgatgaccgg cacgcgccag
    21361 attaatggcc atcggtgtac ccataatgcc taagccaata aatcccagtt tcataattaa
    21421 cctcttttaa atttcgcttt tcctgaattc agacaacacg atcccctgcc aacttagcgg
    21481 cctaaaaaag cattgtctga atagcgttac gttttaacgg tacggatcca tccagcgtaa
    21541 accggcttcc gtggtggttt ggggtttata ttcacaccca acccaaccgt tgtagtcaga
    21601 attttcgatt accttaaaga gataatcata attaatttct ccggttcccg gttcgccgcg
    21661 atgcggatta tcggcaattt gcaggtgacc aattttatca gcccactgag tcatggtgtt
    21721 ggttaattca ccttccatcc gctgcatgtg ataaatgtca tactgaattt tcaaattgca
    21781 gcaaccaaca tcatcaatca atttcagcgc ctgccgagtt ccggtgagat ggaaaccagg
    21841 aatatcaaaa tggttaatag gttcaatcag taataaaata tcttctttca tcagcatatt
    21901 cgcggcataa cgcaggtttt ctacaagcgt tgcgtgaatc tgttcactgc tgaaaccagc
    21961 cggcgtttta ccgaccagac agttaatttt tttattaccc agcgcacggg cataacgaat
    22021 cgctgctgct acgccatccc gaaactcttc ttcacggcca ggaatacagg caataccgcg
    22081 ctcccccgcc gcccagtcac ccgccggtaa attgtgcagc gtatgttcga gtttattact
    22141 cgccagcaca tgttttaatt cttcaatgtc gtagtcataa ggaaacataa attcaacgcc
    22201 gcgaaaacca cactgcgcag ctttctcaaa acgggcgaga aaatcatatt ctccaaataa
    22261 catcgataaa ttagcagaga aacgtaacat tatttatctc ccttattcat agtgcatgaa
    22321 gcaggtttca gtcggtgcgt ccgctgcgtt atcggcgata tcttcaaatt ccatgacgtt
    22381 atccagttcg ctgcccatcg aaatattggt cacacgctcg agaataactt ccacgactac
    22441 cggtacccga tattgcgcca ttaaggcttt cgcctgttca aaggctggcg caatatcttc
    22501 cggtttgaag acccgaatag ctttacaacc taaaccttcc gctactttta cgtggtcaac
    22561 accgtagcca ttcacttcac tggagttgat attctcgaaa gcgagttgca cgcagtagtc
    22621 catgtcaaaa gcgcgttgtg actgacgaat caggccgaga taggcgttat tcaccagcac
    22681 atggatgtac ggaatgttga actgcgcgcc aacggctaac tcttcaatca ggaactggaa
    22741 gtcaaagtcg ccagaaatcg ccaccacatt gcgtttcgga tcagcggcac aaacccccag
    22801 cgccgccgga atcgtccagc ctaacggacc agcctgacca cagttgatcc agtggcggtc
    22861 tttaaagaca tgcagcattt gtgccgcagc gatttgtgac agaccaatgg tggtgacata
    22921 gcaaacatcg cgaccaaagg ctttgttcat ctcttcatac acgcgctgcg gtttcaccgg
    22981 cacgttgtcg aaatgggttt tgcgcagcaa agtgcgtttg cgctgctggc agtcggcgac
    23041 ccattcttta cgacacggca gacgacccgc tttttgcatc tcctgcgcca cttcaaccag
    23101 cagtgtcagc gccgctttag catcagagac aatgccgaga tccggacaca gcacgcgacc
    23161 aatttgcgtc ggctcaatat cgatatgaac gattttgcgc ccttcggtgt atttctctac
    23221 cgaaccggta tgacggttag caaaacggtt accgatacca aacaccatgt cggacgccag
    23281 caacgttgcg ttaccgtaac gatgcgcggt ttgcagaccc accatcccgg ccatcagttc
    23341 atgatcgtcc gggatacagc cccagcccat cagcgttggg atcaccggaa cgctggtcag
    23401 ttcagcaaac tgttgtaaca gtgcagctgc gtcagcatta attaccccgc ccccggcaac
    23461 aatcaccgga cgttcggcct ggattaacat ttctacagct ttttcgatct gcatacggct
    23521 ggcagcaggt ttgtagaccg gcagcggttc gtacatgtca ggatcaaact cgatttccgc
    23581 aacctgaacg tcgaacggta aatccaccag taccggaccc ggacgaccag aacgcatcag
    23641 gtgaaatgcc tgttgcagca cgcgaggcac cagcgccgct tcacgaactg taaccgccat
    23701 tttgctgacc ggtttagcaa ttgcttcaat atctacggcc tgaaaatctt ctttatgcag
    23761 acgggcgcgc ggtgcctggc cggtaatgca cagaatagga atggaatcag cagaagcgga
    23821 atagagcgcg gtgatcatgt ccgtgcccgc aggaccggaa gtccccagac atacgccgat
    23881 atttcctgcc gttgcgcggg tataaccttc cgccatgtgc gaagcacctt ccacatgacg
    23941 cgccagaatg tgacgaatac cgccgtgctt acgcatcgct gagtagaacg gattgattgc
    24001 agctcccgga acaccgaagg cggtggtgat accttctttc tccagcacat acattgccgc
    24061 gtcaacggct ctcatttttg ccattttatt cccacctcta tttattggaa aatttttcca
    24121 actttcaaat tttgtcaaaa aaacccgctc cgcagagcgg gatgcgacag acattatgga
    24181 tgtgctttca gtcccaacgc cgtgctgata tcgcgggcgg tgtctctgac cagctcaccc
    24241 tgactgacaa aacgatcttc tgtcagtctt gatgaaggcc cggagatgga gatagcggca
    24301 acaacactac cgacatcatc gtaaattgct gaagctatgc aattcagacc tacaacatgc
    24361 tcttctttat ctacggtata gcccagttca cgcgcttgtt ccaggtcctt cagcaaggtg
    24421 ggcatatcca caagcgtagt tggcgtaaac tgctgcaaac cggtttgcag aatgatgctc
    24481 atcaactcct cttccgccag cggataaagc agcgctttgc ccgcaccgga agcatgcagt
    24541 ggcagacgac tgcccagtgg cgcacacatc ctgaccatcg atttacactc taactgacca
    24601 attaataccg cttcattgcc gttacggatc gcgacattga ccgtttcgcc ggaaagtaac
    24661 atcaggcggc gcataaacgg cccggcgacg gagaggacat cgcggttatg gatgtacgcc
    24721 gcaccgacgt taaagacacc taatcctata tgccaccagc ctaattgact gtcctgatag
    24781 acaaaatccg ctgcctgtaa aaccttcagc aagcgaaagg tcgtggagag cggcaaatcc
    24841 agattgagag aaatatcgct aaccgacgaa cttcccccac ttttttccaa atattgcaga
    24901 atcgcaattc cccgctctaa cgcctgtgcg cccttctgtg ccacaggctc cgcctgtcct
    24961 ggcctgccgc gccgtctaac ttccgtcata cctacgttaa ctccgaccta caagtgagca
    25021 taaacaatga acaattagtt tatcttatgc aagtcggttg caggcgtcac gcaaaacaga
    25081 gttcctgttc aggaatactt tcaacatcac agttgtcact gccgccgcga tcgatggtca
    25141 gaaaatcggt gacgcgctgc caggcgaaaa gtgggtgatg ccagacgtta cgatggtaat
    25201 tcactccctg ttcgccgttg gtgataaacg cccgcagcgt tgacaggtct ggtttgtcgt
    25261 cacctaacgc cacgaccacc acaaacacct cacctttcat cgggataaag gcctgagtac
    25321 ccagcggatg acgttcgagt tcgtgaatgg tcagcggcag attcgccggt tgcgcgcggt
    25381 taatgctgat aagcgtacgg tcttgctcaa gaatctcaac cagcgccaaa tcgtggtaac
    25441 gctccaccag gccattgtta atatggaaaa aatcccgttg ctgcgtttcg attacgtcgc
    25501 cataagcact aaaggcttcc tgacttaacg gtaatacctg aagtttcatc tttttgtcct
    25561 tgtaacataa ttccgttcaa ccaccgccga ccggtcagct tggtttcaat aacaggcaat
    25621 ctaaaacctt aagagaacaa catcaataca aaagttggaa tatttttcca aaactagatt
    25681 tagtccacaa attgaacagg gttatcatgt aaaaatactg ataaatcaga ggataagaaa
    25741 atcacgcaac ttgcggattt aaaaggggat acctgaagat gttcgatcca gaaaccttgc
    25801 ggactttcat tgcggttgct gaaacaggaa gtttttcaaa agcggcagaa cgattatgta
    25861 aaaccacggc gacgatcagt tatcgcatta aacttctgga agagaatacc ggagtagcgc
    25921 tgtttttccg tacgactcgc agcgtgacgt tgacagcggc tggcgagcat ctactttccc
    25981 aggccagaga ctggctgagc tggctggaaa gtatgccaag cgagctgcaa caggtgaatg
    26041 atggcgtgga acgccaggtg aatattgtca tcaacaacct gctctacaac ccccaggccg
    26101 tcgcccagtt gctggcgtgg ctgaatgagc gttacccctt tacccagttt cacatctccc
    26161 gacaaatcta tatgggcgtc tgggactcgc tattgtacga aggtttttcg ctggctatcg
    26221 gcgtcacggg aactgaggcg ctggcaaata cctttagtct tgatccctta ggatcggtgc
    26281 aatggcgctt tgtcatggcg gcggatcatc cgctggcgaa cgttgaagag ccgctaacag
    26341 aagcgcagtt gcggcgcttt ccggcggtca atattgaaga cagcgcccgc accttaacca
    26401 aacgcgtcgc ctggcgattg ccagggcaaa aagagattat tgttcccgat atggaaacga
    26461 aaatcgccgc ccatctggcg ggcgttggca ttggtttttt gccaaaatcg ctttgccagt
    26521 caatgatcga taatcaacaa ctggtcagcc gggtaatccc aacgatgcgc cctccttcgc
    26581 cattgagtct ggcatggcgc aaatttggca gcggcaaagc ggtagaagat attgtgacct
    26641 tgtttaccca gcgcaggccg gaaatcagcg gatttttaga aattttcggc aacccacgca
    26701 gttaaaaatc aagtttcctt gcgcgttggc gcgtaaaata gcgcgcaagc tgatttgagg
    26761 ttcactttta actatgcaag agagacacac ggaacaggac tatcgtgccc tgctgattgc
    26821 tgatacgccc attattgatg ttcgcgcccc tatcgagttt gagcaaggcg caatgcccgc
    26881 cgctatcaat ctgccgttaa tgaataacga tgaacgcgcc gccgttggca cctgctataa
    26941 acagcaaggc tcagacgcag cgctggcgct gggacataaa ctggttgcgg gtgaaattcg
    27001 tcagcagcgc atggacgcct ggcgggcagc gtgcctgcaa aatccgcaag gtattctctg
    27061 ctgcgcccgt ggcggtcagc gctcacatat tgtgcaaagc tggttgcatg cagcggggat
    27121 tgattatccg ctggtggaag gcggttataa ggcactgcgc cagaccgcga ttcaggcgat
    27181 tattgaactg gcacaaaaac cgatagtgct gattggcggt tgtaccggca gcggtaaaac
    27241 gctgttagtg cagcaacagc cgaacggtgt tgatctggaa gggttggcgc gtcatcgcgg
    27301 ttcggcgttt ggtcgcacgt tacaaccaca acttagccag gcgagttttg aaaacctgct
    27361 ggctgccgaa atgctaaaaa ccgacgcccg tcagaatttg cgcctgtggg tgctggaaga
    27421 cgaaagccgg atgatcggtt cgaatcacct gccggaatgt ctgcgcgagc gaatgactca
    27481 ggcggcgatt gcggtggtag aagatccgtt tgagatccgt cttgaacgcc tgaacgaaga
    27541 gtatttcttg cgtatgcatc atgattttac ccacgcttat ggcgacgaac agggctggca
    27601 ggaatattgc gaatacctgc atcacggact ttcggcgatt aagcgtcggc tggggttaca
    27661 gcgctataac gaactcgctg cccagctgga tacggcgctg acaacgcaac tcaccaccgg
    27721 cagcaccgac ggtcatctgg cctggctggt gccgttactt aaagaatatt acgacccaat
    27781 gtatcgctat cagcttgaga aaaaagcgga aaaagttgtc tttcgcggtg agtgggcgga
    27841 agtggcggaa tgggttaagg cgcagtgatg ggcggtattc tgaaacggcg attgtcgtga
    27901 gaacagaatg gttgcaccag aatacagata taaaaaaacc gacatgacag tcggtttttt
    27961 tattttttcc gcccaagact tggtggttcc gggcagaaat gaaacagtcc tcatctagca
    28021 agtgaattat aagtcaggat gcctacatat tcaaacagct atgttggcga tacaatcccg
    28081 acgctagtag tgatactgcc tcaagggatt accgcagtgc ggaaaagtac ttttaagtcc
    28141 tggtcaggat acaggtggtg ctgattctgg tgctctaatt tggagagagg cagtcctcat
    28201 ctagcacatg aagaaaatca tagttacagg caacgtaagg gtaagctgtg attgtctttc
    28261 tcccttaagg gagcaacgga tgaaaacttt tctcgataaa gttctcatcg ttcttaaggc
    28321 gctaatcgcg ctgctggaac tgatccgtca gtttattgaa taactgagga aacgtagcta
    28381 aggccgaagg tttaccacct tcggtcttta aaaaaaccga catgacagtt ggtttcatat
    28441 ttattgccaa aatgcacact gttcatgccg gatgcggcgt aaacgcctta tccggcctac
    28501 acaatcttgc aaaatcaata tattgcacgc tcttcgtagg cctgataagc gtagcgcatc
    28561 aggcaatcta acacctgtca tcaccccgca aactgcctga acagcgcctt acccttaacc
    28621 agtctcgcgc ccagccagcc gccgaaaagc gacagcagca gcgcaccgct gcacggcaga
    28681 acaatccaca atcgccagtc tggctcccac gggaaatcaa acactttcgc ctgcaacact
    28741 gccagtgccg tttccgcacc aattgcggcc accaggccgg aaacaaaccc aagcatggcg
    28801 aactcacacc acaatgtcgt gcgcagcagt tttttccccg caccgagtgt gcgccatacc
    28861 accagctcct gatgacgctg gcgcattccc acctgcacct gtgccagcaa cagcaacata
    28921 ccgcaggcgg tgaccagcac caccatcacc tccagcgccc gacttacctg ctccagcacc
    28981 tgaccgacct gttttaaaat cgcgccaata tctaacaggc taatggtcgg gaactggcgg
    29041 ttgagttgcg tcaacatgcc gttgccattc tcccagcgga aactggtaag ccagctctgc
    29101 ggttgcccgt ctaatgcccc ttccgggaaa ataaaataga aattaggccg cagactttcc
    29161 cagtccactt tgcgcaggct ggtcacttta gcgcggaact cctgggtatc gcccataaaa
    29221 gtcacggtat caccgagggc aacgtttaag cgtttcgcca gcccctcttc catcgacact
    29281 tcatcggctt ttggcggcca gttaccggcg acaatcggat tatgatcggg ccgcgtattt
    29341 tgccaggtaa gattgagttc gcgattaagc gcctcatctt cattaccttc tgtcggctta
    29401 tcgttaatcg ccgtcagccg cgcccgcact accggataaa atgattccgg gactatatga
    29461 tgttccgcga ggaacgcttt tagcggtgct acctgttctg tggcgatgtt aattaaaaag
    29521 tagttcgggc tttctggcgg tagctgctgc tgccagcggt cgagcagatc gccacgcaat
    29581 accagcaaca gcgccagcag cataaaggag agcgaaaatg ccgaaagctg acttaacgtt
    29641 gaccacggct gacgtaacag gcggctaacc gccaggcgca gaggcagcga tttcagcgtc
    29701 atgcggcgaa gtacattcag cagcatccag cccagcacac cgcacagcaa agccagtact
    29761 accgcgcccg ccagcaccgc ccaaagcagc atgctgccac ccattaatcc ggcgagcagc
    29821 agcacaacca ccacactgac aatcggcaga taaaacttca gcggccagac gttcgctacc
    29881 acatcattac gtaatacgcg taaaggctgc gttgccagca acaagcgata tggtcgtagc
    29941 cccaccagca gcgagatgac cgtcatggtg ccaagcgccc acagccacgg ccagaggctg
    30001 gctggcggta gtgcagcagg cagaacgggc ttgagcagca ccatcaacac gttttcgaac
    30061 aacagaccta tggccccacc ggtaacggct gaaagcgtca gcaccatcaa ccactgaccg
    30121 acgattagct tacgcagttg cgctcgccct gcccccagcg ttttgaggat cgccaccaga
    30181 tcgtagcgac tgcgacagta atgattcatc gctaccgcca ccgctgccac tgccagcagc
    30241 aaggtcagaa gcgccgaaag cagcaggaac tgttgcgagc gttccatcga tcgccccagc
    30301 gcgccttcgt cctgttccag accgtaccag cgttgttcgg gtttaagctg aggtaacaac
    30361 catttctcat agccgtcgag ctggttctcg ttgccgccga atttataacg ccaggtgacc
    30421 cgactccccg gctgcacggc tccggtttta tcgacatccg ccagattcat catcagacgc
    30481 ggagccatct ggaaggggtt aaaaccggaa tccggttcct gaatcacttc tccggcaatc
    30541 cgcaaggtgg catcgcccac gtcaatggtg tcgcccgttt tcaggttaag cagtgccatc
    30601 aggcgtgggg ccagcaatac gctgcccgcc tgcggtttca ggccaggggg attagtttgc
    30661 agatcgccat acatcgggta gatatcatcc accgctttga cgttcgccag ctgcggtgtg
    30721 tcgcctgcaa aggtcattgt ggcgaaagtc agctgcttgc cgactttcag gccgcgcttt
    30781 tgcgcttcct ccagccacgc ttgcggcact tcgcgtgaac tgcgcaacgc ccgatcgccc
    30841 gccataaact cacggctttg ctggcttaag cccttctcca tgcgatcgct gatattgccc
    30901 agcgccagca cgcaggccac cgccaggctt agcgccagcc agacaattaa tagcgacggc
    30961 gaacgccatt cgcgccagaa ccaacgtgca atcatgcttc ctcctgcaac tgcccgttca
    31021 ccagccgtaa gcagcggtcg cagcgtgccg ccagttgcag gtcgtgggtc accataatca
    31081 acgtggtgcc atgttcacgg ttgagggaaa acagcaggtc ggcaatttta tcgcccgtct
    31141 ggcggtcaag gttgccggtg ggttcgtcgg caaacagcac atcaggtcga ccattaaagg
    31201 ctcgtgccag cgccactcgt tgctgttcac cgccggaaag ctgtgccgga agatgatcca
    31261 gacgtttacc cagccctaac tgttcgagca acgctttcgc cccgttacga ctttccgcgc
    31321 tactctcacc gcgcagcaga gccggaagct cgacgttttc cagcgcgtta agggtaggaa
    31381 ttaacataaa tgactgaaaa acaaagccga cgtgcttcgc gcgcaacttt gcccgcgctt
    31441 cttcgtccat attatgtagc ggttgtccca ccagactcac ttcgccactg ctgccgtcat
    31501 caagcccggc gaggatcgcc agcaaggttg acttacccga tcccgactcg cccaccagtg
    31561 cgatggtctc gccacgtttg acaaccagct caactccggt gaggatggag agttcatgct
    31621 ccccctgacc gacggacttc ttaagatgat gaacttcaac aatgttttcc gctggcattt
    31681 gcccttcctg ttcctggtcc tgttaacctt ccgtgccgcc gcagcggaca cgttattgat
    31741 tctgggtgat agcctgagcg ccgggtatcg aatgtctgcc agcgcggcct ggcctgcctt
    31801 gttgaatgat aagtggcaga gtaaaacgtc ggtagttaat gccagcatca gcggcgacac
    31861 ctcgcaacaa ggactggcgc gccttccggc tctgctgaaa cagcatcagc cgcgttgggt
    31921 gctggttgaa ctgggcggca atgacggttt gcgtggtttt cagccacagc aaaccgagca
    31981 aacgctgcgc cagattttgc aggatgtcaa agccgccaac gctgaaccat tgttaatgca
    32041 aatacgtctg cctgcaaact atggtcgccg ttataatgaa gcctttagcg ccatttaccc
    32101 caaactcgcc aaagagtttg atgttccgct gctgcccttt tttatggaag aggtctacct
    32161 caagccacaa tggatgcagg atgacggtat tcatcccaac cgcgacgccc agccgtttat
    32221 tgccgactgg atggcgaagc agttgcagcc tttagtaaat catgactcat aaagcaacgg
    32281 agatcctgac aggtaaagtt atgcaaaaat cggtcttaat taccggatgt tccagtggaa
    32341 ttggcctgga aagcgcgctc gaattaaaac gccagggttt tcatgtgctg gcaggttgcc
    32401 ggaaaccgga tgatgttgag cgtatgaaca gcatgggatt taccggcgtg ttgatcgatc
    32461 tggattcgcc agaaagtgtt gatcgcgcag ccgacgaagt gatcgccctg accgataatt
    32521 gtctgtatgg gatctttaac aatgccggat tcggcatgta tggccccctt tccaccatca
    32581 gccgtgcgca gatggaacag cagttttccg ccaacttttt cggcgcacac cagctcacca
    32641 tgcgcctgtt acccgcgatg ttaccgcacg gtgaagggcg tattgtgatg acatcatcgg
    32701 tgatgggatt aatctccacg ccgggtcgtg gcgcttacgc ggccagtaaa tatgcgctgg
    32761 aggcgtggtc agacgcactg cgcatggagc tgcgccacag cggaattaaa gtcagcctga
    32821 tcgaacccgg tcccattcgt actcgcttca ccgacaacgt caaccagacg caaagtgata
    32881 aaccagtcga aaatcccggc atcgccgccc gctttacgtt gggaccggaa gcggtggtgg
    32941 acaaagtacg ccatgctttt attagcgaga agccgaagat gcgctatccg gtaacgctgg
    33001 tgacctgggc agtaatggtg ctcaagcgcc tgctgccggg gcgcgtgatg gacaaaatat
    33061 tgcaggggtg agttgaagcg cgcgcttaag cccccatgtc aaagaaaata tcgacaacag
    33121 agagcgactc catgtccgta gaaaatattg tcaacattaa cgaatctaac ctgcaacagg
    33181 ttcttgaaca gtcgatgacc actccggtgc tgttctattt ttggtctgaa cgtagccagc
    33241 actgtttgca gttaaccccg atcctggaaa gcctcgcggc gcagtacaac gggcaattta
    33301 ttctggcgaa gctggactgc gacgcggagc agatgattgc cgcccagttt ggtctgcgcg
    33361 cgattccgac cgtgtatctg ttccagaacg ggcaaccggt agatggcttc caggggccgc
    33421 aaccggaaga ggagatccgc gccctgctgg ataaagtgct gccgcgcgaa gaagagctga
    33481 aagcgcagca ggcgatgcaa ctgatgcagg aaggcaatta caccgatgcc ctgccattgc
    33541 tgaaagacgc ctggcagttg tcgaatcaga acggggagat cggcctgctg ctggcagaaa
    33601 cgctgattgc gctgaaccgt tctgaagatg cggaagcggt gctgaaaacc attccgttgc
    33661 aggatcagga cacccgctac caggggctgg tggcgcaaat cgaactgctg aagcaggcgg
    33721 ctgatacgcc ggaaattcaa cagttgcaac agcaggtggc ggagaatcca gaagatgccg
    33781 cactggcgac gcaactggcg ctgcaactgc atcaggttgg gcgcaatgaa gaggcgctgg
    33841 agttgctgtt cgggcatctg cgtaaagatc tcaccgccgc agacggtcag acgcgtaaaa
    33901 cgttccagga gatcctcgct gcgctgggta cgggtgatgc actggcgtcg aagtatcgcc
    33961 gccagctgta tgcattgttg tattgatatt ccgttgatcg gcacatgaac cacaacgccg
    34021 gatgcgatgc tgcgcatccg gcatcgcatc atttcttctt caattgcgtc accaccaact
    34081 ggtggcgcga attataaaac ttacgatagg ttaaatagca ggcaataatg gtcgacaagc
    34141 tggcggttga gagcagcata aaggtcacca taatctgata tttaatcgcc ttcaccggat
    34201 caatcccggc aaatatcagc ccggacatca ttcctggtaa actcactaag ccaaccgttt
    34261 ttgctgaatc gaccgtcgga attaaagccg cgcgaatact gtcgcgaatc aatatcgctg
    34321 aagcctgctt cggcgtcgcg ccaagactca gtttctcctg aatctgttgc tgttcgctaa
    34381 tgacccgttg ccctaaattg ttgtaacaca accccaccgc taccatggcg ttaccggcaa
    34441 tcatcccggc gatagggatc acctgcatcg ggataaattc aatcgaccct gagagaatca
    34501 gcaccgccag ggtaattccc gccccgaccg taatggcaat aaacgatgag ataaaagctt
    34561 tagcaatata tttactgcgt ttttgcgcgt tccacgccgc attaaagcag ataaataaca
    34621 ccatcaataa tgtcaggctg gcatcatcca cgctgaaaat atacttcagc acatagccga
    34681 caataatcag ctgaattatc gctcgcccga cgctccagag aatatctttc tccagcgcca
    34741 gtttttcttt atggctaatt aagattgcca ccaccaccag cattaatgcc agtgctaatg
    34801 attcgttagt aatattatgc gagttcatag cgtgcttcct gcatttctcc ggcatgcggt
    34861 tgcagtgtaa tcactttatc cgcatgatta atttcgtctt tatcgtgtgt cacccacagc
    34921 acggcaatat tttgctcgcg cacataacga tggatcatct cattgacgtt atgtttatta
    34981 ctttcatcca gcgcactggt tatttcatcc agcaataaaa ccttcggcat aaattgcagg
    35041 ttacgaatca atgagatgcg ttgtttttca ccaccagata gctcggcgat atccttcgtc
    35101 aaaatgctgt ccggcaaagc gaagcgttcg agaaaatcga gaaaaatggc cgggtcaggc
    35161 tgctggttac ggatctgcca gggaaagatc agattatcgt ataccgtatc gccaaacagc
    35221 gtcggtgtct gggcgcagta agagacttgc tggcggtaga tttctggctt tagtgtgctg
    35281 acatcctccc cttcaaacag taacgttccg ctggttgggc tgatcaatga agcaactatt
    35341 tttagcagcg tacttttgcc acaaccagaa ggaccggtaa ttaacttaaa ttcgccagca
    35401 cgcagcgaaa aattgatgtt attaagaatc ttcgtatcac ccgccagata tcctacgttt
    35461 tgtagctgaa gcaaaggact attttcctgc atcgctgttc cctttttctg atttttacta
    35521 aaaacagttt atccttcgca ggaataaggg ggaactctct ttcagtaatc aggtataatt
    35581 tgtttaatca agcagcaatt ttgctgatta tggacggtac aacaggaggt ttttccgatg
    35641 cttatcttta tcccgattct catttttgtc gcgctggtca ttgtcggcgc gggcgtcaaa
    35701 atcgtgccgc agggctatca atggacggta gaacgttttg gtcgctatac caaaacgtta
    35761 cagccggggc tcagtctggt ggtgccgttt atggatcgca ttggtcgcaa gatcaatatg
    35821 atggagcaag tgctcgatat cccttcccag gaagttatct cgaaagataa cgccaacgtt
    35881 accatcgacg cagtctgttt tattcaggtg attgacgcgc cgcgcgcggc ttatgaagtc
    35941 agcaatctgg agctggcgat catcaacctg accatgacta acatccgtac cgtgttgggt
    36001 tcaatggaac ttgacgaaat gctctctcag cgcgacagca tcaactcacg cctgctgcgt
    36061 attgtcgatg aagccaccaa cccgtggggg attaaagtca cccgtattga aattcgcgac
    36121 gtgcgcccac cggcagagct tatctcttca atgaacgcgc agatgaaagc ggaacgtacc
    36181 aaacgcgctt acattcttga agcggaaggg atccgtcagg cggaaatcct caaagccgaa
    36241 ggtgaaaaac agtcgcaaat cctgaaagcg gaaggcgaac gtcagtcggc gtttttacag
    36301 gctgaagcgc gtgaacgttc cgccgaagca gaagcccgcg ccaccaaaat ggtgtctgaa
    36361 gctatcgcct ccggtgatat tcaggcggtg aactacttcg tagcgcagaa atacaccgaa
    36421 gcgttacagc agatcggttc ctccagtaac agcaaagtag tgatgatgcc attagaggcc
    36481 agcagcctga tggggtcgat tgccgggatt gccgagctgg tgaaagacag cgccaacaaa
    36541 cggactcagc catgatggag ttaatggtcg ttcatccaca tattttctgg ctcagtctcg
    36601 gcggtttgct gctggcagcc gagatgctgg gcggaaatgg ttatttgttg tggagcggcg
    36661 tggcagcggt gattaccggc ctggtggtct ggctggtgcc gctgggttgg gagtggcaag
    36721 gggtaatgtt tgccatcctg acgctgctcg ccgcctggct gtggtggaaa tggttgtcgc
    36781 ggcgggtgcg cgaacaaaag cacagcgata gtcatttaaa ccagcgcggg cagcagctga
    36841 ttggccgacg ttttgtgctg gaatctccgc tggtcaacgg gcgcggtcat atgcgcgtcg
    36901 gtgacagttc atggcctgtc agcgccagcg aggatctcgg tgcaggtacg catgttgaag
    36961 tcattgcgat agaagggata accctacata tccgagccgt ctcgtcttaa tcaccctgcc
    37021 cgatgatgac agcagccgga gagattttcg ataatcgggc agtcggcgct gtcatcgcca
    37081 gggcaggcat tcgccagtgc cagcagctgg tcgcgcatgg attgcagctc ctcaatgtgt
    37141 cgttcgatct ccgccacctt ctccagcgtg cgccgtttga cgtcggcgct gtgccgctgc
    37201 gggtcgttaa acagattcac cagctcgccg ctctcttcca ggttaaagcc cacctgccgt
    37261 gcctggcgca gtaaggtcag ttcgttgaga tgctgctgcg tgtaggtgcg ataaccgttt
    37321 tcgctgcgca tcggcggcgt caccagcccc ttctcttcat agaagcgaat ggctttgctg
    37381 gtcaggccgg taatttttgc tacatcgctg atgttcatcg ttcgcgcaac gcctcctgtg
    37441 ctttgttaaa gggtttcaac agatattcaa gcacgttttt ttgccagtgc gaatatcgac
    37501 cgccaccaca ataatacagg aagtactgcg cacagagaga tgttatacgg ttttattgcg
    37561 cttcatgacc attgccacaa taaggctgag taagagtgag ccaataatcc atatcaacgc
    37621 acggctaccc tgggaccaca gactgtagat aaagccaacg atcaccaaca acatcaccaa
    37681 cgtaccaacg ataagaatcg gcaacgaggc gtgaatatca tgacgcaggc ggatcgccac
    37741 cacaaacacc gccaggtagc aaatcaaaaa ggtggcgctg gcaacgctgg cgagtgagcc
    37801 taaattcagt gccgccgtca tcagcataat caacaccacg acaataatgt tgccccaggt
    37861 actctgccgc cacagggatt tattcattag cttcggcagt tcgcgttcgc tgcccatgtt
    37921 gtccatgatg ttaaacacgg cgaacaggtt cgcgttaatg gctgaagccg tcgccagtaa
    37981 agcgccgatg acgacgatca cataacccac atgcccgagc agcggagaag cagcctgcgc
    38041 tacggcggta tcggcatatt tttctaactc taatgccgat acatcgctaa gcaaaaccag
    38101 tgccagcgag atataaagca acgtggtaac gccaatcgcc accagaaacg cccgtggcat
    38161 aatgacctgc ggatctttca ctttatccgc cgcgttcgcc atcatgccaa agcccgcata
    38221 ggcaaggaaa gttatcccaa tacaggagaa gaacgcaccg gagctggggg gcgcagagac
    38281 ggaaatatgc gccggttgca gcgaccagac accggcaata atcagcaata acaggatcat
    38341 cattttaatg ccgacgagga tcacttccag ccgccctacc gcatggttgc ttaaggagtt
    38401 gaaaagcgtc atcaccgcaa tgatccccaa cgcgtagagc aaaataaggt gctcctcctg
    38461 gctgccttca tgcaaaaact gcacggcata agcgccaaaa gcacgggcga ccatggcgat
    38521 gctcaccgcc agcgtcaaca ggtacagtaa cgagagcgcc agcgaaaaga cgccgttgcc
    38581 taatccgcga cgaaagaagt cgataatgcc gccattgctg ggatagctcg cccccagacg
    38641 cgcataggca taaccggaaa acatcgccac aataccgcca aaagcaaagg cgacccaggt
    38701 cgaggcttcc attagcaatg cagcctgccc cagcagcgcg aagatccccg cccccaccat
    38761 tgccccaatg ccgatggaaa cgacgttcca tagaccgaga ggtttgttac cgttattacc
    38821 ttccgtgttc atcatgatca gcccttaaac acgttatagc cgagttggtt agcgaccgat
    38881 gccaccattt tttgaccgcg aacactgttg ccttcttcgt ccagcggtgg tgagaacgcg
    38941 gcaattccca tcacgccagg gacgaccgcc agaataccgc cacctacacc gcttttgccc
    39001 ggtaaaccaa cgcgatacgc ccagtcaccg gagcgaccat acagcccttc catcatcatt
    39061 tcggccagaa tgtacggcac gttgtcggcc tgaagaacac gtttatgcgt cagcggattc
    39121 acaccacccg ccgccagcgt cgcgccaagc gttgccagtt caacagtatt gatgagcgtg
    39181 gagcactgac gggtgtacac gtcacaggct tccattgcat cacaatagag atatccggcg
    39241 gagtacagca gccaggcaat ggcccggtta tggaagttgg ttgtttgttc cgactggttg
    39301 acttcatcag agagcgctac ctgctcacca gccagttgct gttggatatg taaaattcgc
    39361 tgccagcgtt gttcaatatt ttcagcgtta atcaggctgg tggtggcaat agcgccagca
    39421 tttaccagtg gcgacagtgg tttgccgcca tgcaactcta aggcgataac tgagttaaag
    39481 ggcaatccgg tcgggtcagc gccaattttg tcctgtaccg cctgcgggcc gacatcttct
    39541 aacgcaaggg ctaacgtaca gactttcgag atggattcca gcgcaaagcg gtaatcacta
    39601 tcacccgcgc gatagatgtt gccatcgcag gtcacaatag ccactgctgc cagttgacca
    39661 ggtacattcg ccagaaaggg aatgtagtcg gcattttgtc cgccgttaag tgagtgaaat
    39721 tgggtgtaag cctgatccac tgcctgctgt aatttgtttg catctaacat cttttgttaa
    39781 ctccttttta tagatgcggg aggtaattcc tcaccccggt gccgattttc aggcatcctg
    39841 atttaactta gcacccgcaa cttaactaca ggaaaacaaa gagataaatg tctaatcctg
    39901 atgcaaattg agccaatttt ttaatcttta cggactttta ctcgcctggt ttattaattt
    39961 cttgaccttc cccttgctgg aaggtttaac ctttatcaca gccagtcaaa actgtcttaa
    40021 aggagtgttt tatgtcacaa actatcgacc tgaccctgga cggcctgtcc tgcggtcact
    40081 gcgttaaacg cgtgaaagaa agtcttgaac agcgcccgga tgttgagcag gcggatgtgt
    40141 ctatcactga agcgcacgtt accgggactg ccagtgcaga acagctgatc gaaaccatca
    40201 aacaagcggg ttatgacgca tctgtaagcc acccaaaggc taaaccgctg gcggagtcat
    40261 caatcccgtc ggaagcactg acagcggttt ctgaggcgct tccggcagcg accgccgatg
    40321 acgatgacag ccagcagttg ctgctgagcg gcatgagctg cgccagctgt gtcacccgcg
    40381 tacaaaatgc gctgcaaagc gtaccgggcg tcactcaggc acgggtaaac ctggcggagc
    40441 gtactgcgct ggtgatgggc agtgcctccc cacaagattt agtgcaggcg gtggaaaaag
    40501 cgggctacgg tgcggaagcg attgaagatg acgctaaacg ccgcgagcgc cagcaagaaa
    40561 ccgccgtagc tacgatgaag cgcttccgct ggcaggcaat tgttgcactg gctgtgggta
    40621 tcccggtaat ggtctggggg atgatcggcg ataacatgat ggtcaccgct gacaaccgca
    40681 gcttgtggtt agttatcggc ctgataaccc tggcagtgat ggttttcgcc ggcggccatt
    40741 tttaccgcag tgcatggaaa agcctgctga acggtgcagc gacgatggat acgctggtgg
    40801 cgctgggaac gggtgtggcg tggctctatt cgatgagcgt caacctgtgg ccgcagtggt
    40861 tcccgatgga agcgcggcat ctttactatg aagccagtgc gatgattatc ggtctgatta
    40921 atctcggcca tatgctggaa gcgcgcgcac gtcagcgttc ttctaaggcg ctggagaaat
    40981 tactcgattt aaccccaccg acggcgcgag tggtcaccgc agagggtgaa aaaagcgtgc
    41041 cgctggcgga agttcagcca gggatgttac tgcgcctgac gaccggcgat cgcgtgccgg
    41101 tagatggcga gattacccag ggcgaagcat ggctggatga agcgatgctg acgggcgaac
    41161 caatcccaca gcaaaaaggc gaaggcgaaa gcgtccatgc cgggacagtg gtacaggacg
    41221 gcagtgtgct gtttcgtgcc agtgcggttg gcagccatac cacgctgtca cggatcattc
    41281 gcatggtgcg ccaggcccag agcagcaagc cagaaatcgg tcagctggcg gataaaatct
    41341 cagccgtatt tgtacctgtg gtggtggtga ttgcgctggt cagtgcggca atctggtact
    41401 tctttggtcc ggcaccgcaa attgtttata ccctggtgat tgccaccacg gtactgatta
    41461 tcgcctgtcc gtgtgcgctg gggctggcga cgccgatgtc gattatttcc ggcgtcgggc
    41521 gggcggctga gtttggcgtg ctggtgcggg acgccgacgc gctgcaacgc gccagtacac
    41581 tcgataccgt cgtgttcgat aaaaccggga cgctgaccga agggaagccg caggttgtcg
    41641 cagtgaaaac atttgctgat gttgatgaag cgcaggcatt gcgtctggcg gcggcactgg
    41701 aacaaggttc cagccatccg ctggcacgag cgatcctcga taaagcaggc gatatgcagc
    41761 taccgcaggt caacggtttc cgcacattgc gcgggctggg cgtgagcggt gaagctgaag
    41821 gtcatgcgtt attgctgggc aatcaggcgt tgttaaatga tcagcaagtc gatactaaag
    41881 cgattgaagc cgatatttca gctcaggcat cgcaaggagc aacgcctgtg ctgctggcga
    41941 ttgacgggaa agcagtagcc ctgctggctg tacgcgatcc attgcgtagt gatagcgtgg
    42001 cggcgctgca acgcctgcat aaagcgggat atcgtctggt gatgttgacc ggggataacc
    42061 caaccaccgc caatgcgatc gccaaagaag cagggattga tgaggtgatc gccggggtgc
    42121 tgccggacgg taaagccgaa gcgatcaaac atctgcaaag tgaaggacgt caggtggcaa
    42181 tggtgggcga cggcattaac gacgcgccag cgctggctca ggcggatgtc ggcattgcga
    42241 tgggtggcgg cagtgatgtt gccattgaaa ccgcggcgat taccctgatg cgccatagcc
    42301 tgatgggcgt tgcggatgcg ctcgctattt cccgcgcaac gctgcacaac atgaagcaga
    42361 acctgctcgg tgcgtttatc tacaacagta tcggtattcc ggtcgccgcc ggtattttgt
    42421 ggccgttcac tggaacactg cttaacccgg tagttgccgg agcggcaatg gcgctctcgt
    42481 cgattaccgt agtgagtaac gccaaccggt tgctgcggtt taaaccgaag gaataagtac
    42541 atcattgcgg atgcgctgct ttagggtggc gcatttggca tcgacgcatg actgccggat
    42601 gcggcgtaaa cgccttatcc ggcctacggt tcaggcacag gtttgtaggc ctgataagac
    42661 gcgtaagcgt cgcatcaggc aatggtgctc ggatgccgga tgcggcgtaa acgccttatc
    42721 cggcctacgg ttcaggcaca ggtttgtag
//
LOCUS       DAPHBJ010000014        41879 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.014, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000014 DAPHBJ010000000
VERSION     DAPHBJ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.014"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1726
                     /gene="adeD"
                     /locus_tag="RHZ76_000729"
     CDS             <1..1726
                     /gene="adeD"
                     /locus_tag="RHZ76_000729"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDV2811421.1"
                     /translation="AEYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGRY
                     IAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTVI
                     CDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLAW
                     RDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYITAGIE
                     NCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPWE
                     IAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLSD
                     ARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQFTPGK
                     RYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRLAPACGLLGGFGLNE
                     GALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAGLM
                     STDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEKFA
                     FTTLEVTE"
     gene            complement(1771..3162)
                     /gene="uhpT"
                     /locus_tag="RHZ76_000730"
     CDS             complement(1771..3162)
                     /gene="uhpT"
                     /locus_tag="RHZ76_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HDV2811422.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3300..4619)
                     /gene="uhpC"
                     /locus_tag="RHZ76_000731"
     CDS             complement(3300..4619)
                     /gene="uhpC"
                     /locus_tag="RHZ76_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HDV2811423.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4629..6131)
                     /gene="uhpB"
                     /locus_tag="RHZ76_000732"
     CDS             complement(4629..6131)
                     /gene="uhpB"
                     /locus_tag="RHZ76_000732"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HDV2811424.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6131..6721)
                     /gene="uhpA"
                     /locus_tag="RHZ76_000733"
     CDS             complement(6131..6721)
                     /gene="uhpA"
                     /locus_tag="RHZ76_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HDV2811425.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6796..7086)
                     /gene="ilvN"
                     /locus_tag="RHZ76_000734"
     CDS             complement(6796..7086)
                     /gene="ilvN"
                     /locus_tag="RHZ76_000734"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDV2811426.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7090..8778)
                     /gene="ilvB"
                     /locus_tag="RHZ76_000735"
     CDS             complement(7090..8778)
                     /gene="ilvB"
                     /locus_tag="RHZ76_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HDV2811427.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8884..8982)
                     /gene="ivbL"
                     /locus_tag="RHZ76_000736"
     CDS             complement(8884..8982)
                     /gene="ivbL"
                     /locus_tag="RHZ76_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HDV2811428.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9547..9636
                     /gene="tisB"
                     /locus_tag="RHZ76_000737"
     CDS             9547..9636
                     /gene="tisB"
                     /locus_tag="RHZ76_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HDV2811429.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            9916..11100
                     /gene="emrD"
                     /locus_tag="RHZ76_000738"
     CDS             9916..11100
                     /gene="emrD"
                     /locus_tag="RHZ76_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HDV2811430.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11108..11605)
                     /locus_tag="RHZ76_000739"
     CDS             complement(11108..11605)
                     /locus_tag="RHZ76_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDV2811431.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11602..11964)
                     /locus_tag="RHZ76_000740"
     CDS             complement(11602..11964)
                     /locus_tag="RHZ76_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HDV2811432.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(11954..12301)
                     /locus_tag="RHZ76_000741"
     CDS             complement(11954..12301)
                     /locus_tag="RHZ76_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HDV2811433.1"
                     /translation="MNISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(12472..13680)
                     /locus_tag="RHZ76_000742"
     CDS             complement(12472..13680)
                     /locus_tag="RHZ76_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2811434.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKGLERAYKNFFQKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSLPGRNVRAKSGLNRSILDQGWYEIRRQLAYKQLWRGGQVLAVPPAYTSQRC
                     VCCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            13750..13872
                     /locus_tag="RHZ76_000743"
     CDS             13750..13872
                     /locus_tag="RHZ76_000743"
                     /inference="COORDINATES: protein motif:HMM:NF013921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2811435.1"
                     /translation="MHVHLVFVTRYRRQIFDYDATEKLRTYFSNVCADFEAELV"
     gene            13873..14118
                     /gene="tnpA"
                     /locus_tag="RHZ76_000744"
     CDS             13873..14118
                     /gene="tnpA"
                     /locus_tag="RHZ76_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDV2811436.1"
                     /translation="MDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRPDIAV
                     RYYYKGVLWSPGYFANSCGGAPISVIRQYIEQQQTPG"
     gene            complement(14249..15571)
                     /locus_tag="RHZ76_000745"
     CDS             complement(14249..15571)
                     /locus_tag="RHZ76_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDV2811437.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGAR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMPQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALAPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSASVAKAILDELLEANKAYWPELR"
     gene            complement(15571..17187)
                     /locus_tag="RHZ76_000746"
     CDS             complement(15571..17187)
                     /locus_tag="RHZ76_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDV2811438.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            17483..18199
                     /locus_tag="RHZ76_000747"
     CDS             17483..18199
                     /locus_tag="RHZ76_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV2811439.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(18196..19857)
                     /locus_tag="RHZ76_000748"
     CDS             complement(18196..19857)
                     /locus_tag="RHZ76_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HDV2811440.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(20053..20481)
                     /gene="ibpB"
                     /locus_tag="RHZ76_000749"
     CDS             complement(20053..20481)
                     /gene="ibpB"
                     /locus_tag="RHZ76_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HDV2811441.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(20593..21006)
                     /gene="ibpA"
                     /locus_tag="RHZ76_000750"
     CDS             complement(20593..21006)
                     /gene="ibpA"
                     /locus_tag="RHZ76_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HDV2811442.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(21000..21072)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            21312..21644
                     /locus_tag="RHZ76_000751"
     CDS             21312..21644
                     /locus_tag="RHZ76_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDV2811443.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(21646..22860)
                     /locus_tag="RHZ76_000752"
     CDS             complement(21646..22860)
                     /locus_tag="RHZ76_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HDV2811444.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIVE
                     GGKMSNLDAMDITAPYTPGVLRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPHGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTEHHANSPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            22961..24025
                     /gene="cbrA"
                     /locus_tag="RHZ76_000753"
     CDS             22961..24025
                     /gene="cbrA"
                     /locus_tag="RHZ76_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HDV2811445.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLIGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMNAFQFQFGKTVKSEKCTVLFPSRWQDFVCGKENAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            complement(24022..25359)
                     /locus_tag="RHZ76_000754"
     CDS             complement(24022..25359)
                     /locus_tag="RHZ76_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2811446.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGHRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQELLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(25434..26582)
                     /gene="dgoD"
                     /locus_tag="RHZ76_000755"
     CDS             complement(25434..26582)
                     /gene="dgoD"
                     /locus_tag="RHZ76_000755"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HDV2811447.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(26579..27196)
                     /gene="dgoA"
                     /locus_tag="RHZ76_000756"
     CDS             complement(26579..27196)
                     /gene="dgoA"
                     /locus_tag="RHZ76_000756"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HDV2811448.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(27180..28058)
                     /gene="dgoK"
                     /locus_tag="RHZ76_000757"
     CDS             complement(27180..28058)
                     /gene="dgoK"
                     /locus_tag="RHZ76_000757"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HDV2811449.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREEKTPVVMAGMVGSNVGWKVAPYLSVPACFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(28055..28744)
                     /gene="dgoR"
                     /locus_tag="RHZ76_000758"
     CDS             complement(28055..28744)
                     /gene="dgoR"
                     /locus_tag="RHZ76_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HDV2811450.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            29022..29678
                     /locus_tag="RHZ76_000759"
     CDS             29022..29678
                     /locus_tag="RHZ76_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2811451.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(29724..30536)
                     /gene="yidA"
                     /locus_tag="RHZ76_000760"
     CDS             complement(29724..30536)
                     /gene="yidA"
                     /locus_tag="RHZ76_000760"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDV2811452.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMELPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(30651..31049)
                     /locus_tag="RHZ76_000761"
     CDS             complement(30651..31049)
                     /locus_tag="RHZ76_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HDV2811453.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(31289..33703)
                     /gene="gyrB"
                     /locus_tag="RHZ76_000762"
     CDS             complement(31289..33703)
                     /gene="gyrB"
                     /locus_tag="RHZ76_000762"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDV2811454.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(33732..34805)
                     /gene="recF"
                     /locus_tag="RHZ76_000763"
     CDS             complement(33732..34805)
                     /gene="recF"
                     /locus_tag="RHZ76_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDV2811455.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(34805..35905)
                     /gene="dnaN"
                     /locus_tag="RHZ76_000764"
     CDS             complement(34805..35905)
                     /gene="dnaN"
                     /locus_tag="RHZ76_000764"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDV2811456.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(35910..37313)
                     /gene="dnaA"
                     /locus_tag="RHZ76_000765"
     CDS             complement(35910..37313)
                     /gene="dnaA"
                     /locus_tag="RHZ76_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDV2811457.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSMRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            37610..37690
                     /locus_tag="RHZ76_000766"
     CDS             37610..37690
                     /locus_tag="RHZ76_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2811458.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            37920..38060
                     /gene="rpmH"
                     /locus_tag="RHZ76_000767"
     CDS             37920..38060
                     /gene="rpmH"
                     /locus_tag="RHZ76_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDV2811459.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            38077..38436
                     /gene="rnpA"
                     /locus_tag="RHZ76_000768"
     CDS             38077..38436
                     /gene="rnpA"
                     /locus_tag="RHZ76_000768"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDV2811460.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTIAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            38400..38657
                     /gene="yidD"
                     /locus_tag="RHZ76_000769"
     CDS             38400..38657
                     /gene="yidD"
                     /locus_tag="RHZ76_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDV2811461.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            38660..40306
                     /gene="yidC"
                     /locus_tag="RHZ76_000770"
     CDS             38660..40306
                     /gene="yidC"
                     /locus_tag="RHZ76_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDV2811462.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            40412..41776
                     /gene="mnmE"
                     /locus_tag="RHZ76_000771"
     CDS             40412..41776
                     /gene="mnmE"
                     /locus_tag="RHZ76_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDV2811463.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        10005 a        11427 c        10787 g         9660 t
ORIGIN      
        1 ggctgaatac caggaattgt tagccgtttc ccgtggcgac gctgttgccg attatattat
       61 tgataatgtc tctattctcg acctgatcaa tggcggagaa atttccggcc caattgtgat
      121 taaaggacgt tacattgccg gtgttggcgc agaatacgct gatgctccgg ctttgcagcg
      181 gattgatgct cgcggcgcaa cggcggtgcc agggtttatt gatgctcacc tgcatattga
      241 atccagcatg atgacgccgg tcacttttga aaccgctacc ctgccgcgcg gcctgacgac
      301 cgttatttgc gaccctcatg aaatcgtcaa cgtgatggga gaagccggat tcgcctggtt
      361 tgcccgctgt gccgaacagg caaggcaaaa ccagtactta caggtcagct cttgcgtacc
      421 cgccctggaa ggctgcgatg ttaacggtgc cagttttacc cttgaacaga tgctcgcctg
      481 gcgggaccat ccgcaggtta ccggccttgc agaaatgatg gactaccctg gcgtaattag
      541 cgggcagaat gcgctgctcg ataaactgga tgcatttcgc cacctgacgc tggacggtca
      601 ctgcccgggt ttgggtggta aagaacttaa cgcctatatt actgcgggta ttgaaaactg
      661 ccacgaaagt tatcagctgg aagaaggacg ccggaaatta caactcggca tgtcgttgat
      721 gatccgcgaa gggtccgctg cccgcaatct caacgcgctg gcaccgttga tcaacgaatt
      781 taacagcccg caatgcatgc tctgtaccga tgaccgtaac ccgtgggaga tcgcccatga
      841 aggacacatc gatgccttaa ttcgccgcct gatcgaacaa cacaatgtgc cgctgcatgt
      901 ggcatatcgc gtcgccagct ggtcgacggc gcgccacttt ggtctgaatc acctcggctt
      961 actggcaccc ggcaagcagg ccgatatcgt cctgttgagc gatgcgcgta aggtcacggt
     1021 gcagcaggta ctggtgaaag gcgagccgat tgatgcgcaa accttacagg cggaagagtc
     1081 ggcgagactg gcacaatccg ctccgccata tggcaacacc attgcccgcc agccagtttc
     1141 cgccagcgac tttgccctgc aatttacgcc cggaaaacgc tatcgggtca ttgacgtcat
     1201 ccataacgaa ttgattacgc actcccactc cagcgtctac agcgaaaatg gttttgatcg
     1261 cgatgatgtg agctttattg ccgtacttga gcgttacggg caacggctgg ctccggcttg
     1321 tggtttgctt ggcggctttg gactgaatga aggtgcgctg gctgcgacgg tcagccatga
     1381 cagccataat attgtggtga tcggtcgcag tgccgaagag atggcgctgg cggtcaatca
     1441 ggtgattcag gatggcggcg ggctgtgcgt ggtacgtaac ggccaggtcc aaagtcatct
     1501 gccgttgccc attgccggcc tgatgagcac cgacacggcg cagtcgctgg cggaacagat
     1561 tgacgccttg aaagccgccg cccgtgaatg cggtccgtta cccgatgagc cgtttattca
     1621 gatggcgttt ctttctctgc cagtgatccc cgcgctaaaa ctaaccagtc aggggctatt
     1681 tgatggcgag aagtttgcct tcactacgct ggaagtcacg gaataataaa aaagcccggc
     1741 gtcatgccgg gcaaaagtca ccagttacgt ttatgccact gtcaactgct gaattttttt
     1801 ctcgcggcgg attttgcgtt cttccatcac cgccactatc gccatcaggc agatacaacc
     1861 aatcgcggcg atatcaagtg cggcgaaggt gcctgcccag ccggtaaggc cgaataccgg
     1921 cgtcccatcg gcaatcattc ccagacctaa cttggcaaag ctgtcaccaa tcaggtaagc
     1981 aaaggtgcct ttaataccat cggcagcgcc aatcgctttt ttaggtacaa agccaacagc
     2041 agccacacca atcaacaatt gcgggccaaa gaccaggaaa cccaacgcaa agagagaagc
     2101 cagatagata tattcgttgc tggcatgttg atacacaccg agcgtggcga taatcagcgc
     2161 cagcgcgatg caggccacca ggccacggcg accgttcgcc aggtcagaga gccagcccca
     2221 cagcagcgta ccgaccagcg caccagcttc aaacagcgta aagccctgaa tcgccaccgc
     2281 tttagagagt ttcagttcct ggaacgcgta tacggttgac cactggtcga taccaatacg
     2341 taccacatag aggaaaatgt tggcgaagca cagcagccag atcactttgt ttttcagcac
     2401 atactcaaca aagatctgcc acttggtcat atcggtagat tctgtctctt tgtcctcttc
     2461 gctgatctcc tcgccgaaca gttcttcagc tttgccgagg ccataagatt ccggggagtc
     2521 gctaccgtaa cgcaggccga taaaaccgac aatcagcgcg ataatcgacg ggaagataaa
     2581 catgccgatg acatggccat cgaacaggta atttgccccg aacagcgcca cacctgctgc
     2641 gcctgcaccg ccaaggttgt gagaaatatt ccagaaaccg aggaatgtcc cgcgtttacg
     2701 acgcggcgtc catttggtga tggtggagta actgcacgaa ccgccggtac tctggaaaaa
     2761 gccgcttaag gcgtagaagg caatcatcag gaacaggcta accgagccgc tgcccatact
     2821 ggcactgaag cccagcatac aaatagcaga gaggataagc atgaacggca ggaattgttt
     2881 ggtgtttttg ccgtcggcgt agtatgaaac caacgtttta cccacgccat aagtgatgga
     2941 gaaacccagg ccgatcatcc ccagctgcgt catgctcaac ccgtaggtcg aaatcatatc
     3001 gttctgcgcg atgttaaagt tcttgcgaat caggtacatc gtcaggtagc cgataaagac
     3061 caccaggtag gattgcatga acggtttgaa ccacattttg cgccgcactt cgagcggaag
     3121 gtccagggtc ggcttgcgaa cctggtttag gaaagccagc atgggttact cctgaaatga
     3181 atacctgcgt gaggcatgca ttgtaaaaat cagcagcaaa tcaggcctga gatagcgtcc
     3241 aggaaaaacc tggaaaattt cttagttttg ccggatatga agtgaaaagg tgagatgcat
     3301 cacgcttcac gcggtgtctg ggcgttcaaa aagggcagta acagcagtgc ggaaatcccg
     3361 gcggcgatag agataaccac aaaaaatccg ctccagtgcc aggtatcgag cactttcgcc
     3421 agcggccaac cagcaagcga cgcccccaga taagcaaaca agccgacaaa ccccgtcgcc
     3481 gcccctgccg cctctttgtg ggaacactct gccgccgcca taccaattaa catctgcggg
     3541 ccaaagacaa aaaaaccaat ggtgaagaag caggttgcct gcatcacgta gctggcaaat
     3601 ggcatcagcc acagggagcc gactgaaagc aaaattccgg cggcgaaaat caaattcatc
     3661 ggccctcggt tgccgttaaa caatttgtcc gagccccaac ccgctaccag cgcaccgata
     3721 aatccaccca gttcaaacat cgtcactgcc gtattcgccg tgaccagatc gacgcccagc
     3781 gtctcggaca tatacaaatt gccccagtcg ttgatcgccg cccggaccac atagaccagc
     3841 acatagcaaa acgaaagcag ccagatatac ggattcagca acacatattt ggtgaggatc
     3901 tctttacgcg tcagccctgc cccttcttgt tgttgagcta tctccagcgc gtcgtgtcgc
     3961 cattcaccga ccgccggtaa acctaacgcc tgcgggcgat cgcgcagccg ccagcagaga
     4021 aaaattccca cgacgatcgc catacaacca gcaatcatca tcccggcacg ccagccgtaa
     4081 tgtagtgcag ccgctgccat cacaatggga atgagtgcgc cgccgacgtt atgcgccgtg
     4141 ttccataatg cccaccaacc gccgcgctcg gtacgagaat accaggccgt taacagacgc
     4201 gcacacaccg gtgaacccca gccctggaaa aaggcgttca gcacccagag cacggcaaac
     4261 gcccatagcg acgtcgagaa gccaaacaga atgttgatga tgccagtggc gataagccct
     4321 atccccataa aataacgggc atttgagcga tcgctgacaa tgccggagac aaacttcgac
     4381 acgccgtaag taatgtaaaa cagggtcgct aataggccga tatcgctacg gctgagcacg
     4441 ccgttagcaa ggatttctgg tacggcggcg ttaaaacttt tacgcgtgaa gtaaaacagc
     4501 gcgtaaccca gccagatggt cagcagaata tgccgacgcc agtagcgata acgggcatca
     4561 atttcatgtt tatcagtcat taatggcgca tcggcaggcg ctttcagaaa cggcaacatc
     4621 atcgaacctt agacataacg ttgaggtaga gaaacgctga cacgcgtgcc gtgcagacag
     4681 gaaatgtgta atgtgccacc cagcgccgtt acgcgctcgc gcattccggt gaggccaaaa
     4741 ccttgttgcc cggaacccgg cggcaaaccg ctgccatcgt cttcaataac cagcatcaac
     4801 cgttcatcct gctgccagcc ttgcagggtg accgcgctgg catcagcatg tttcacaatg
     4861 ttgttcagcc cttcctggca gacacgaaac agcgtcacgc gctggttttc gcttaacgct
     4921 gattcatcga ttcgccattc gagatggctg acaataccgc gcccttccag ctccatttcc
     4981 cgcatcagtg agcggatggc ctgctccagg gtgagatcat ccaactggcg cggacgtaac
     5041 cgacccaaca aacggcgcac cgcgtcgtaa acgcccagcg atagttgttc gatgagctgc
     5101 ccgctctgct tcacgctggc gttatctgcc gccagccgct gaacaatgcc cgcctgagta
     5161 cgaatagcag tgatggtctg accgatatca tcatgcagct cacgcgccac atcacggcgc
     5221 acgctctctt cggtttccag caaccgttca gccagatgct gattgcgcgc cagttccttt
     5281 tgcagcgact ggttaagttc acgcaaccgc tggatgccag cgccaagcaa caaccctgtc
     5341 agactttgca ccagcagcga gagcaataaa tccaccggat gatcgcgcca ggtttgactg
     5401 gcgatcagcg cgatggcgtt catcaacgtc gcaatcagcg ccccttgcca accatagtgc
     5461 caggccagcg cgataatcgg cagcgccaga cagaatggcg taaagcgcga cagttcgtcc
     5521 ggcaatccca actggagcca gagactgata acaaacagca gcaagtacca gaccagatgt
     5581 cgcccgcgcc agttgattgg ctgagaaacc agtgacgggc cgagcggcag ccaggtgtta
     5641 ttggcgagat agtgccagaa caccagacat atcggggcca gcgtcaggcc gccagttaaa
     5701 gtcagcaaca gcgcattcca cgactctttg ccgtgccaaa gccagggcag cgactgcaac
     5761 aacgccgccg ccgttaacgc cgccccctgt aacagcaagg tgcgccagtc acgctgatgg
     5821 cgatagcgcg agatcagcgc tacgggcagt aacgtcagta aactaccgat catcaataac
     5881 ggaaaatggg ttaaaccgac cgcctgcgtt agccagtaaa tcagcagcca ctccgcgccc
     5941 agcagcaccg gccagtatcc gcgcgggcat tgcagcatta gccccagacg cagaccaaac
     6001 ggaaataaca gcaccgccat atcagggcgc tcaaccagat gcaggctgat actccacagg
     6061 caaaaccatg cggcagagaa gataaaaaag caggcaataa cggtaattaa gcgggagaac
     6121 aacgtcttca tcaccagcca tcaaacatgc ggcgcgccag ctctacgtcg ttactgacgc
     6181 ccagtttttc catcagattg gcgcgatgga cgtgtaccgt tttcggtgac aagcccagtt
     6241 cggcggcaat ctctttcacc gccattcctt gcgccagttt ttccgccacc tggcgttcac
     6301 gtttggttag cgggtcctga cgaccggatg ccagtttaat ggcaatatcc ggcgtcagat
     6361 aacagccgcc cgtggcaacc gtatgcaccg cagcaatgag ttcatccggg ctacagcgtt
     6421 tggaaagaaa gccgcgtgcc cccgcgttaa gcgcctgctc aaccagcgca ggactgtcgt
     6481 gaacggagag cataatcgtc gccatacctt tcggcagctg gcttagcagc tccagaccgg
     6541 agatatcggg catggagata tcgcaaatac acacctgcac accgcgcccc ggcagccccg
     6601 ccagcgcctc gcgccccgaa ccaaactcgg caactacctg caaatcaggt tccagcccca
     6661 gcagctgcgc aaagccggag cggacgatga ggtgatcgtc tataagggca acggtgatca
     6721 tggtcttgtc ctggcgggta aaaaaaaacg cgcttacctt aacgataagc gcgatgttgt
     6781 tcaagccttg agcggttact gaaaaaacac cgcgatcttg ttaaacatcg tcggatcgga
     6841 ctgattacgc tgcactttca cgacatcttc cagcttatcg atttggctta tcatctgctc
     6901 cagacgctgg tcgtcattga ccagtagcca gatatggctt ttgtcgctgt cctgaatcgg
     6961 cagacaaaga atgccttcaa cgttaaaagc gcggcgggca aaaaggccac aaacgtgggt
     7021 cattacgccc ggatggttgc gaacggtgag ctccagaatt acgttgtcat gagttgtgtt
     7081 ttgcatggct tattccccca ccatttcagt attcgccgca cctggcggca ccatcgggta
     7141 aactttttct tcggcatcaa tgcgcacatg gatcagcgcc gggccagggc gattgatgat
     7201 ttcctgcaat gcagcctgcg gatcggcttc gttattcaaa tcacaggttt cgaggccgaa
     7261 tccggcggca atctgcataa agttgatttt gcccggatag gtggcggcaa aaacgccttg
     7321 ctcgtagaac agactctgtt gctgatgcac cagccccagc gcttcgttgt tcatcagaat
     7381 gattttgaca tccagctgat tttcactggc ggtcgccatc tcctgaatat tcatcatcag
     7441 gctgccgtcg ccggagaaac acaacacttt gcgatccggg ttcgccagcg ccgcgccaat
     7501 cgccgcaggc aggccaaaac ccatcgtgcc cagcccaccg gaggtcagcc actggcgtgg
     7561 gcgattgagc ggataagctt gcgcggtcca catctgatgc tgccccacat cggtggtgat
     7621 aatcgcattg tcatcgacac aggcggcaac ggcgttgatc aggccgtaat ggcttaatgg
     7681 atcgcacgct ttcgggattg gacacggaaa ctcacgctgc aaatccgcta ccaactggtg
     7741 ccactctgca cgcggttgcg cttccaccag cgggatcaac tgcgccagca cgtcatcaac
     7801 atccgcctga atcgccacat gcggctgctt gattttaccc agctctgcac ggtcgatatc
     7861 gacatgaatg attttggcat tcggacagaa ctgctcggtt ttgccaatcg cccggtcatc
     7921 aaaacgcgca ccgagcacaa tcagtaaatc cgcctcctgc aagatatagt tagtgctgcg
     7981 cacgccgtgc atccccagca tacccagcga caacggatgc gcttttggca gcatgcccag
     8041 cgccattaaa gtcatggtgg taggcagttg cgctttctcc gccagttcac gcacccgcgc
     8101 aggcgcattg atcacaccac cacccagata aagcaccggg cgtttggcag cgttaatcat
     8161 tgtagctgcg tcacgaatgc tttcttcgct aaaggcgggt gcagcggctt tttctgccac
     8221 cgcgggctga gcttcaatct caaaaaccgc cgtttgcaca tccttaggaa tgtctatcca
     8281 caccgggcct gggcggcctg attgcgcaat gcggaaggca tcgctcatga cctgcgggag
     8341 ttcttcgata tgtctgacca gatagttgtg tttggtgatg gggatagaga tgccgtaggt
     8401 gtcgacttcc tggaaggcgt cggtgccgat catcgaggcg ggaacctgac cagtgatgca
     8461 aatcagcggg atggagtcca gccgcgcatc ggcaatggcg gtcaccaggt tagtcgcacc
     8521 cggtccgcta caggccatac agaccgccgg tttaccgtcg gtgcgcgcca ttccctgagc
     8581 gataaatccc gcgccctgtt catggcgagc cagaatatgg cggatttgcg tactttggct
     8641 taaggcatcg taaacaggca ggatagaacc gcccggaatg cccgtcacaa tcttaatgcc
     8701 ctgctgttcc aggaaatgaa cgataaattc tgcgccggta aagcgcttac gcgtcgatgt
     8761 tgtgcccgaa cttgccatgc tccagtcctt ttcttctggg ccgactttcc gggaggtgct
     8821 taaacgaaaa accccgcccg gtttgcgccg gcggggtttt ggaatcgtgt gttgttccag
     8881 tccctacggc gcattgccga cgaccaccac cacacgcacg acgaccactg cggcggatgg
     8941 cgcagttggt agtagttttg cgttgaacat ggaagtagtc attggggacc ttgttggttt
     9001 tgtgtttaac aatatttata caagcacagc tttacagggg agacaatgga aaatttttca
     9061 gcaagggaaa attgaggggt tgatcacgtt ttgtactgaa ttgcagataa caaaaaaccc
     9121 cgccggagcg aggtttcgtc agtcgcctgc ggctggtaac cgcaaagcac actgtattat
     9181 gtcaacactg aaagtatacg tgttccgcgc agaacgcgca atttcggcac gaattttgac
     9241 gtatttagtg catagttgag tatcgatcac agtttgcgtt ttgtccaaat attactgttt
     9301 atttatacag taaacttcta taatatcact gtacgcaatg tgttatgcgg gggccgcatc
     9361 gttacccggc gcactaagtc ctggctgaaa cgggtggtgc cgtcagcgcc ttaaccccgc
     9421 gtgagcacac tgtgttatgt caacaagcac aacgtttctc cttgagatac cgcgtgcaca
     9481 acagctggca acaggcagcg gaaaggtacg tcagctggca gtgctcctga accacaggag
     9541 acgcgtatga gcctggtgga tatcgccatt cttatcctca aactcattgt tgcagcactg
     9601 caactgcttg atgctgttct gaaatacctg aagtaattca gattcaagtc gcaccaaagg
     9661 ggagcgggaa accgctcccc ttttatattt agcgtgcggg ttggtgtcgg atgcgatgct
     9721 gacgcatctt atccgcccta ccatctctcc cggcaacatt tattgccgct tttgtttaca
     9781 tattctgccg ctaaacaatt ccccattcct ggcgtatatc tggctaacat tcatcaatgt
     9841 gatagattcc tctcccgcat ttatgggaat gcgtagtgac ttattctaat tatttttata
     9901 aaagcatccg tgataatgaa aaggcaaaga aacgtcaatt tgttattgat gttggtatta
     9961 ctcgtggccg tcggtcagat ggcgcaaacc atttatattc cagctattgc cgatatggcg
    10021 cgcgatctca acgtccgtga aggggcggtg cagagcgtaa tgggcgctta tctgctgact
    10081 tacggtgtct cacagctgtt ttatggcccg atttccgacc gcgtgggccg ccgaccggtg
    10141 atcctcgtcg gaatgtccat ttttatgctg gcaacgctgg tcgcggtcac gacctccagt
    10201 ttgacggtgt tgattgccgc cagcgcgatg caggggatgg gcaccggcgt tggcggcgta
    10261 atggcgcgta ctttgccgcg agatttatat gaacggacac agttgcgcca tgctaacagc
    10321 ctgttaaaca tggggattct cgtcagtccg ttgctcgcac cgctaatcgg cggtctgctg
    10381 gatacgatgt ggaactggcg cgcctgttat ctctttttgt tggttctttg tgctggtgtg
    10441 accttcagta tggcccgctg gatgccggaa acgcgtccgg tcgatgcacc gcgcacgcgc
    10501 ctgcttacca gttataaaac gcttttcggt aacagcggtt ttaactgtta tttgctgatg
    10561 ctgattggcg gtctggccgg gattgccgcc tttgaagcct gctccggcgt gctgatgggc
    10621 gcggtgttag ggctgagcag tatgacggtc agtattttgt ttattctgcc gattccggca
    10681 gcgttttttg gcgcatggtt tgccggacgt cccaataaac gcttctccac gttaatgtgg
    10741 cagtcggtta tctgctgcct gctggctggc ttgctgatgt ggatccccga ctggtttggc
    10801 gtgatgaatg tctggacgct gctcgttccc gccgcgctgt tctttttcgg tgccgggatg
    10861 ctgtttccgc tggcgaccag cggcgcgatg gagccgttcc ccttcctggc gggcacggct
    10921 ggcgcgctgg tcggcggtct gcaaaacatt ggttccggcg tgctggcgtc gctctctgcg
    10981 atgttgccgc aaaccggtca gggtagcctg gggttattga tgaccttaat gggattgttg
    11041 atcgtgctgt gctggctgcc gctggcgacg cggatgtcgc atcaggggca gcccgtttaa
    11101 gcgcacgtca ccgcagcatc gtcatcagct ccatgggaga acgatgctgc tttatcagat
    11161 cacgcatcac ccgcatatgc ggtgcggagt aagaataaaa cgcctgatag cccgcacaaa
    11221 gcacgctgtc tggcgtctcc tgccgagcgt gcgggcacag gcgatgccag tcgcacgcct
    11281 ggcagtcacg gcgcggttgc gggttttcgc tgccacacca tttttgtaag gtggcgtcaa
    11341 aaagctcaat cgagatacga ctgatatctg cccgcaccca acactcgaac agggcaatta
    11401 aaaatttccc ccacagctct gctgtcaccg aagcggccgt cagatcgccc cggtcatcaa
    11461 attcaagata gggaatgagc tgtacggagc ggatgccccg ctgtttgaaa ttgtgataaa
    11521 aatcggcagg ttgcagagca tccttgtact ctacaaccag tttatgcctg tcctcttcag
    11581 catcaatgac ctgacttcct gtcatgcgac tctctcaata aatacgataa gttgatgtat
    11641 atgcgttaca gcaaacagta ttgcgagaga taacaccgcg agggagatca aaaatttgtc
    11701 acggactacg tgaaattggg aaaaatcgct attcgtgaca tccattaaat tgcgattacg
    11761 ggtgtagtgc cagaggatca gcgccacgat ggcgagaatg ccaatcgaaa tccagaataa
    11821 catgcccgcc tggtgccagt tgtgtttgat agccaacgcc atcaacgcgc cgtagcccag
    11881 catggtacga aaccacgcca gtgatgtgcg ctccggctgc agtcctgggt cggcaatgcg
    11941 tctggctttg cggctatccg gcatacaaca ccagtcccat aacgatcacc gccacgacca
    12001 tcaatattaa gctaatgatt aataagctgt tggtgtaggg caggtcctct ttcagacgca
    12061 tcgctttttc attgcgtaac cagcgcaggt agccatacat tgctaacccg ccggaaaaca
    12121 gacacaacaa caacgccagc agttcacgaa tgacgggcgt ggcgaaatct ggcgcaagtt
    12181 gatcaagccc aacccccgcc gccagaaatc ctagtgcggt acggatccac gccagaaaag
    12241 tacgctcatt agccagcgag aagcggtaat cgggcgcttc tccaaggcgg gaaatattca
    12301 ttgttgctcc tttgcaattt cactaacgct ctatctttag cggttttttt taagaatttc
    12361 gtccgcccgt ttcctgatat gcttttgcca tccgctacaa ggaggtcaga tgggtatcat
    12421 agcgcaaatt gacatcctcc acgccctgaa ggacgtggat ccctgctacg ttcaggctgt
    12481 cgcctgaatc atttcggtgg gttcctgctt caacgagcgg cctgactgca ccatctctcc
    12541 acaggcaaga acggcgtgcc ccgccgctaa aatgttacga gcgccattta catcggcgtt
    12601 cgctgtatat ccacatacct ggcatctgaa tttactttgt gacaggcgat tttctttcgc
    12661 tgtatgacca cagcacacgc aacgctggct tgtgtacgct ggcggaacag caagcacctg
    12721 accaccacgc cagagctgct tgtacgcaag ctggcggcgt atttcatacc agccctgatc
    12781 cagtatcgaa cggtttaaac ctgattttgc ccggacattg cgccccggca ggctgaccgt
    12841 acccgctgct gactttgaca tgttgctgac cttcaaatcc tcaatgacta tcattgcgtg
    12901 gtttttgctg acggtcgttg tgactttgtg aaggtagtcc ctgcggatat ttgcgataca
    12961 ggaatgcagt cgctgtattt tgcgtttctg cttctgccag ttgttgctga atttgacctt
    13021 gcggcttaac tgtcgctgaa gtctcgccag cgtcttctgg tttttctgaa aactgtttac
    13081 aggctcaaag actgtgccat ctgacagcgt ggcgagttta gccacgccag catccagccc
    13141 gatcattgat gctgaagggt gaaccggagt tgatacttca ctttctgtct gaatactgat
    13201 gtaccactta ccgcaggact ggctgacagt gacatttttc acaacacccg tgacctgacg
    13261 gctgttccgg tagcgcatcc agcccagttt cggcagaaaa atacggctgt tttcctgatc
    13321 gagcttaacg ccctgcgggt agcggaatgc atcattctgt ccccgctttt tgaatcgggg
    13381 aaaagcagcc cgcttctgga agaagttttt gtaagcccgc tcaaggcctt tcagtgactg
    13441 ttgcaatggc tgtgagggcg aatctttaag ccattgcgtt tcagtggcgt ttttccactc
    13501 aaccagccag gaagccattt tcccgtaagg gatgtattta ttaccggcct catgattctc
    13561 attctgaagc gccagagcac gattgaaaac gaaacgacat gcgcctgcga agcgcctcat
    13621 ttcacgctcc tgttgaccac cgggtcttaa ctggaattta aatgcctgta atcgtttcac
    13681 aggccaaatg atactttggt ctatgaataa agaaaccgat atccgacggg gaagcattgt
    13741 gttttcctga tgcatgttca cctggtcttt gtcaccagat accgacgtca gatttttgac
    13801 tatgacgcga cagaaaaact acgcacttac ttttcaaatg tatgtgctga ttttgaagct
    13861 gaactggttt aaatggatgg cgaaccagat cacgtccatt tgttaattaa ctatcctccc
    13921 aaactggcga tatccagtct ggtaaacagc ctcaaaggcg tatcgggtag gttactgcga
    13981 cgagatcgac cagatattgc agtcaggtat tactacaaag gcgttttgtg gagtcctggc
    14041 tattttgcca atagctgcgg aggtgcgcca atatccgtca tccgccaata cattgaacaa
    14101 cagcaaacac ctggttaggt ggaaaaccgc gccttatatc cccgccctga aggacgggga
    14161 tataaggcgc accggataaa tttggttatg cgccgtcagc cgtgctaaag aatacggacc
    14221 agcacccaac agatgccagt ccacacaatt aacgtaactc tggccagtac gctttgttgg
    14281 cctccagcaa ttcatccaga atagctttgg cgaccgaggc gctcggtacc gttttcgaca
    14341 acgtaatcgc ctgccacagg tgctgatatg aacgctgttc ccaggcgtcc acgaccagtt
    14401 tttccaccgc cacttgctga ctcattaacc ctttctgaaa ctgcgggata tcgccgacca
    14461 ccagtggttc tggtccgttg tgcccaacca gacacgggat ctcgaccatc gcttcatcat
    14521 caaagttatg aattgccccg ttgttaggca caatcagcaa catccgctcc tgagtgttga
    14581 aggcaattgc cgccgccaga tcgacgatgt atgacgcatg ttcgtcaatt tccagcttgc
    14641 ccgccgctga atttcctgcc gccgtaatgg cgcggcaagc atcgaaaacc tgtttctcgc
    14701 gatgttccat cacctcattc gcgcgggtat gttcagggtt ggaatgctga acgacgtaat
    14761 ccgggaagag ataatatttc agataggtgt ttggtaatgt atccggggcc agtgcctgga
    14821 catcccgcgc ttttgcatag gtgtcgttcc agctcgcttc agtatgcgta tcttgctgcg
    14881 gtggaacata accatgctta gagacatatt gccgcagctg gggcatcagg tcgttgccct
    14941 gcaaatcttc aattgatgtc caccagccaa agtggttcag gccgtagtag cgcacgcgca
    15001 tctgtttgcg atcttgcagc ccaacaattt gcgccatccg gctttcaata ccgattggca
    15061 tgtcacagat gttgaggatt ttcgcattcg ggcgcagacg acgcgtggct tctgcgacaa
    15121 tggctgccgg gttggagtag ttgagcatcc aggcatttgg tgaatatttt tccatataat
    15181 ccaccagttc caggacgccg ccaatggaac gcatgccgta cgctattccg ccgggtccgc
    15241 aagtttcctg accaacaacg ccgtggcgca gcgggatttt ttcatccagt tcgcgcatcg
    15301 ggtatttgcc gacgcggatg tgcgccatga caaaatcaac gtcgctgaat gccacttcag
    15361 gatcggtggt gtaactaaac gcaatgtccg gcgctttttc tttaaggatg actttacagg
    15421 cttcggcaat cacttcctgc cgcgcaccat cgttatcata aaatttcagt gcacgaagcg
    15481 ggaaacggtc ctgattcgcc aggagcatca acacgatgcc tggcgtaaag gtgcttccac
    15541 cgcctgcgac aaccactgag aatttggtca ttatactgcc tccgtaatgg caacattttc
    15601 tgctgattga tgagaattaa ttaagctatc gagctgttca cgcagctgag atacatgcag
    15661 accaatgatc acctgaatgg catcgccact acggaagacg ccgtgcgctc ccagcttttt
    15721 aaagacttcg tcatccagcg tttgtgacat gtcatgcagt gcaatacgta gacgcgtcgc
    15781 gcaattgttg atgctggaga tattgccgac cccgcccagg gcttgcagga taccggcagc
    15841 ctgatccaac tctttttttg gctctgcagc ggtggtttgg cctcgcgagg ctttatattc
    15901 ggcttttgag tagagtttca cttccgcatc ttcacgtccc ggcgtacaca tgttgaactg
    15961 cagtatcagg gtgcggaaaa ccacgaagta cagcagggta aagcacaacc caatggcgat
    16021 ttgggtcagc atcatatccg cgtggttgct gaacatcggg atccagtttt gcggtaaaac
    16081 ctggtcaatc agacctccgc ccatgttgcc caccacacca aagagataca ttacggtcga
    16141 cattgaggcc gcaagcacag cgtgtaccgc aaacagcaac ggtgaaatga acaggaaggt
    16201 aaattccagc ggttcggtaa ttcccaccag catggcggtt aaggttgcgg ggatcagcaa
    16261 gcccgccact tttacccgat tttccggtgc ggcagtgaag tacatcgcta aagaaatgcc
    16321 cacggcacca aagatttttg agttaccgtg cagggcaaaa ccgccttccg ggaacaacga
    16381 tttcagcggc tcggcgctca gactgaactc ttgcagatgc tgcgcccagt acatctgaat
    16441 gccgccttca acagctgccg gaccaaagat aaacggtccg tagatgaagt ggtgtaaacc
    16501 ggttgggatc agaatacgtt cgaggaaggt gtaaacccac accccaagcg cacccgccga
    16561 acgcaggaac gcttgcagag attcaatccc catttgtact tttggccagc cgagcaatgt
    16621 cagccaggca caggggatca tcaccaggaa ggcgataatc accacataag acgtcccctg
    16681 gaaaatgccg agaaacaccg gcagtttttt atcgaacaga cggttatgca gcgccgtcac
    16741 aatgccggaa atgataattg cgccgataat gctggtatcg agggttttaa tcccggccat
    16801 cattgtcaga ccgctacctg ccaccgcgtc ctgagtgaaa tcgacgccga agtaacttcc
    16861 ccaggtcatt cccatcgcat taatgaaata gttccaggtc aggaaactca ccatcaccgc
    16921 cagacaagca cgcccctgcg cttgcttagc aaggccaatg ggtaaaccga cagcaaaaat
    16981 cagcggcata ttacggaata ccgtccaacc gccctcttca ataatgtgta cgatttgcgc
    17041 gaataaactg ttcggatcgg tcagtgattc cccgacaaac atcgggtttt gcaacaagat
    17101 ggcaagaccc accacaatcc cggcgaaggg aaacagcagc actggcgtga acatcgcgcc
    17161 gccaaagcgt tgaatttgac tgagcatttc cacatcctct tttctcaatt ctgtaagtag
    17221 gaagcctgat tggttatgcg gcaggcctgg agcgaggata ggaagatgcg atcggcagaa
    17281 catgacgacc caatgggatt cgtgatcgct ttcatgcttt tcgttgggcg gtagagatct
    17341 acgttttcgc gaaaaagtag acctctgtga gcacaggtac acagcatcga acacatcttt
    17401 aaaaaaaaga tgttttttca atcgattaag cagaacttgt gtgcgcatta cccgggcttg
    17461 caggcaaaaa agagatctag agatgatcta caaaagcatt gcggagcggt taagaattcg
    17521 acttaactcc gcagatttca cgctaaacag ccttcttccc ggtgaaaaaa agctggcgga
    17581 agagtttgcg gtatcgcgga tgaccatccg taaagccatt gacctgctgg tagcgtgggg
    17641 gctggtggtc cgccgccacg gtagtggcac ttacctggtg cgcaaagatg tactgcatca
    17701 aaccgccagt ctgaccgggc tggtggaggt gttaaaacgg cagggaaaaa cggtcaccag
    17761 ccaggtgctg atttttgaaa tcatgcctgc gcctccggcc attgccagcc agttacggat
    17821 tcaaatcaac gagcagatct acttctcccg tcgcgttcgt tttgtggaag ggaaaccgct
    17881 gatgctggaa gacagctata tgccggtaaa actgttccgt aatctttcgc tgcaacatct
    17941 ggaagggtcg aagtttgaat atattgaaca agagtgcggg attttgattg gcggtaatta
    18001 tgaaagcctg acgccagtgc tcgccgatag actgctggcg cggcaaatga aggtagcgga
    18061 acacacgcca ctgctgcgga tcacctcgtt gtcatatagc gagagcgggg agtttttgaa
    18121 ttattcagtg atgttcagaa atgccagcga ataccaggtg gagtaccatt tacggcgact
    18181 ccacccggaa aagagttaac cgatactcca gaagagcacc gccagtaatt ggggggtgat
    18241 aatgcgcagg aacattacca acggatagac agtggcgtaa gagagcgccg ccgcaccgct
    18301 ggttggatga agattattag caaacgccag cgccggagga tcggtcatgg aacctgccag
    18361 catcccgcac atggtcaggt aattcatttt ggctaacatc cgcgccagaa tgccaacagt
    18421 aatcagcgga acggcggtga tcagggcacc ataaccaatc cagcttagcc cttcgccatt
    18481 gaccagggta ttcacaaaat ccccaccaga tttcagacca acgaccgaga ggaacagcac
    18541 gatccccagc tcccgcagcg cgaggttggc gcttggcggc ataaaccagt acagcttgcc
    18601 gatactgccg atacgcccga ggatcaacgc cataatcagc ggtccgcccg ccagccccag
    18661 tttcaacgcg gccgggaatc ctggcacaaa gacgggaata gaacctaaca atacgcctag
    18721 cccgatgcca ataaacactg gcagcatctg aacctgttgc agtttttgct gcgcattccc
    18781 cagcacattg gcaacggcat caattgcgga cggacgcccc accagattaa ggatatcgcc
    18841 gaactgcagg ctgatatcgc cactggcgac cagttcgacc ccggcacggt tcaggcgcga
    18901 gataacaacg tcatagcgtt ctttaaagtg caggtcgcga atacgttttc cgagcacgtt
    18961 ttcattggtg accaccacac gctcgacgcg caaatcagtg cctttcgtgg acagcgaagt
    19021 atcgacctcc tgaccaatca ccagttgcgc attatgtaaa tccgctggct gacccaccag
    19081 atgcagcaaa tcgcccagtt ggataatggt atctggcgaa ggaactttta gggtttcttc
    19141 gcgtttcaga cgcgagcaga taattttgtc gccgttgaga atcggtacat ctttaatggc
    19201 taaatcatgc aggttagggt tctcaacgcg aatattgata gtcttgatca gcgcgccgcc
    19261 attggtgcgt gaagactcgt gctgctgagc ttctgtctcg acattgacgc ggaaaataac
    19321 ccgcaacatc cacatggtga acaaaatccc gcaaatgccg aatggatacg ccatcgcgta
    19381 actcatcccc atctgatcga ccatttccat tggtgtaccc aggtcgcgca aaatctgctg
    19441 ccctgccccc agcgctggcg tattggtaac cgcaccggag aaaatcccca gcactaccgg
    19501 cagtggaata tcaaacagtt tatgcaggat ggcggtaacc agaccaccga tgatgacgat
    19561 cagaacagca aacaggttga ggcgtaatcc ggagacgcgc aatgaggcaa agaagcccgg
    19621 ccctacctga atcccgatag tataaacgaa caggatcagg ccaaattcct gaataacatg
    19681 cagcatatcg ctacttaatg tcatccccgc ctgagaaaca aaatggccga cgatgatccc
    19741 accaaacagc acgccgccaa tacctaatcc tatgccgcga aatttgacgt tgccgataaa
    19801 caaaccgacg actgccacca aagccagaat actgaccgtt aatgctatat cactcatcgt
    19861 ccatttctct tgagataaca ttttaattgc tacggattat ggctgaatcg gtgaagcaga
    19921 tttgccttat agcgcacatt atcacgggaa agaacgtgcc gaaatatctt aaacagtcga
    19981 aatttaacgc attgttgagt aagtaagaca aaaaaagccc caccagaatg gcggggcaaa
    20041 gagaatagct agttagctat ttaacgcggg acgttcgctg atagcgatac gctgcgctgc
    20101 gatgggttca ggctcattac gaattaaatc aatatgcagt aaaccgttta cgaaggttgc
    20161 gccagagact tccatatttt cagccagcgt aaagctcagg ctaaatggct gattcataag
    20221 cccttgatgc agccattttt tctcttcttt tggctgctcc ggcgtgcctt ttacgctcag
    20281 gcgcgtacct tccagttgaa tctctaaatc ttcctgacgg aaacctgcca gcgcaagggt
    20341 aatgcggtag tggttatcgt cgcttttctc aatgttgtac ggcgggaagc tctggctttc
    20401 accggcgttt tgcagtgcgt tggccagttt gtcaaaaccg atccattgac gcatcagtgg
    20461 ggataaatcg aagttacgca tagtcatttc tccttctaag aagcgagtaa gtacctgcaa
    20521 atccgaagat tcgcatatgc tccctgacgg cgagcatgga gatgtcaggc cgcgccaggc
    20581 ggccttaggg aattagttga tttcgatacg gcgcggtttt ttcgcttccg gaatcacgcg
    20641 ttcgagatcg atatacagca aaccatttac caggttagca ccacgaacat gaatgttctc
    20701 agctaactgg aatttgcgtt caaagttgcg ttcagcgatg ccctggtaca gataggtgcg
    20761 ctctttttgt tcgtcggcgt gagcaccttt caccaccagc agattatcct gggcggtaat
    20821 ttccagttcg ctctcagcaa aaccagccac agcgatagca atgcggtaat ggttttcgtc
    20881 taccagttca acgttatacg gagggtagcc gccattactc tggctctggt tgttttctaa
    20941 gtggttaaac aaacggtcaa atccaatagc agaacggtaa agcggggata aatcaaagtt
    21001 acgcataatc aatagctcct gaaatcagcg agaatgtaag accttccaca atggacaggt
    21061 caggtagcca gaacacccat caggcgcgtc ctcatcggct acgatgtaaa aatgggtctg
    21121 gaaatgaact tttcaagcct tatcagcgga cttttttttg cagtttatgg tctattgcat
    21181 agactgaggg ggcagcacga aacgttaaag tgcgatagcc gccacagtgc gacgtaatga
    21241 tgatgttatt ttcagcaagg gatcgctata actcatcagg ccaacaccca cgatcaacag
    21301 atgaatttat tatgataaga aatgtgttgt tagcgttcat gatatgcagc ggaatgacat
    21361 tactcggagg atgctccagc gtgatgtccc ataccggagg gaaagaagga acgtatcccg
    21421 gcacccgcgc cagcgcaacc atgattggcg acgatgaaac caactggggc actaagtcgc
    21481 tggcgattct cgatatgccg tttaccgctg tgatggacac gcttttactg ccgtgggatg
    21541 tgttccgcaa agacagctcg gtgcgctcgc gcgttgaaaa aagcgaagcc aacgcgcaag
    21601 ccaccaatgc ggttattcca ccggccagaa tgcctgataa ctgatttagc gatcagtttc
    21661 ggtgatccac agctggccgc cttccatcca cgccagccac tgaccatccg gcgagaagac
    21721 cacggcgtcc gcagaaggtg aattggcgtg gtgttcggtt aaatactcaa cctcgccgct
    21781 ttgcgcatgg gcacaagcaa ttcgattatc cagcacaaag cccaaccatt ctcctgacgg
    21841 atgccagtta aatgcagact gaatatccgt tttgttatgg gttaactggc gcggctcgcc
    21901 gccctgtggc gagataagcc acagttgcac aatgccgtta tcatcacgca ttaaaaacgc
    21961 gatttgcgta ccatgcggat tacagcgcac ccagtggcgc gggacgttga ctaaccccgg
    22021 ataagcccga tggtgggtaa aggttaaacg tcgctgcacg acgccgcgcg gtggtgcggg
    22081 cagggttgtt tccgttccac ttaacggcgc atcacctgcc gctttccagc cagcttcatc
    22141 ttgcggtaac tcaacgataa acagctccgg cactttctcg ccctttggcg aaagtgtgtc
    22201 gccaataaac gccagcgcgt gatttcctac ccatccttct tcataagcac gattgatttc
    22261 atcgctgcca ggctgcggcg tgggcgtggt tttactcacc agcacgcacc agtggctacc
    22321 gctgtattca cgcggatgct gcttttgtac gttgaccggg ccaaacggcg cagcaacacc
    22381 gacgtttcgc aaatccagcg ccggatcgag ttcatgcatt acatggtcgt tataggtaaa
    22441 gctcaccctt tcaccgttcg ggctaaagac atgcacatgg ctgccgccgc gcagcactcc
    22501 tggcgtatac ggcgcggtaa tatccatcgc atcaaggtta ctcatcttgc cgccttcaac
    22561 aatcactccg cgccgatgat ggaagtcgta atgccatgtt tcatcaggat tttccgggcc
    22621 gtggatgaaa acatatttct ctgacttcgg atgaacggtc accacgccga cgtgtgcgcc
    22681 ctgtgacgcg cgatagataa cctcgacctc gccggtatgg atattcacac gctcaatggt
    22741 ctcgccggta aacgacgcgc cagaaggacg cacgtcaaat accagccact ggctgtcggg
    22801 cgtccaggta ttggtattgg tgagcaggtg attacgggga gcaaaggtta tctgtttcat
    22861 ggcgcggtcc tgatacaaaa cgggtcccgc catcatactt cacaaggact tcactttcag
    22921 acaatagtct tttgccatcg tgatgaatgt gagtgaaaag atggaacatt tcgacgtggc
    22981 gattattggc ctcggcccgg caggatcggc gttggcacga aagttagcag gcaaaatgca
    23041 ggtgatcgcg ctggataaaa agcaccagtg tggtactgaa ggtttcagca aaccttgtgg
    23101 cggtctgctg gcaccggacg cgcagcgatc ttttattcgc gatgggctga cgcttcctgt
    23161 cgatgtgatc gctaatccgc agattttcag cgtcaaaacc gtcgacgtcg ccgcatcgct
    23221 cacgcgtaac taccagcgaa gctatatcaa tattaatcgc catgctttcg acttgtggat
    23281 gaaatcgctg atccccgcca gcgttgaggt ttatcacgac agcctgtgtc gaaaaatctg
    23341 gcgtgaggat gataaatggc atgtcatttt tcgcgcagac ggttgggagc agcatattac
    23401 tgcccgctat ctgatcggtg ccgatggcgc aaactcgatg gtgcggcgac atctctaccc
    23461 ggatcatcaa atccgtaaat atgtcgctat ccagcagtgg ttcgcagaga aacatccggt
    23521 gccgttctac tcctgcatct ttgataatgc gataactgac tgttactcat ggagtatcag
    23581 caaagacggt tattttatct ttggcggtgc ttatccaatg aaagacggtc agacgcgttt
    23641 cacgacgctg aaagagaaaa tgaacgcctt tcagttccag tttggtaaga cggtgaaaag
    23701 cgaaaaatgc acggtgctgt ttccctcgcg ctggcaggat tttgtctgcg gtaaggaaaa
    23761 cgcctttctg attggcgaag cggcaggatt tatcagcgcc agttcgctgg aggggattag
    23821 ctatgcgctg gatagcgcag acattctgcg ttcggtgtta ctgaagcagc cagagaagct
    23881 caataccgct tactggcgcg ccacccgcaa actgcgttta aaactcttcg gcaagatagt
    23941 aaaaagccga tgcctgaccg caccggcttt aagaaagtgg attatgcgca gtggtatggc
    24001 gcatattcca cagttgaaag attagccaac gcgcttcaca tcgcccacca gcaggatgta
    24061 agagagcgcg ccaatcaacg cgacggcgga gatataaacc agtgcaggtg cgaaaccgta
    24121 accctgcgcc aggtacccca ccaccagcgg aacggtgatg ccgcccagac caccggcgaa
    24181 gttaaacacg ccgccggtta aaccaatcag gcgcatcggt gccagagaag agaccagcga
    24241 ccaggtaatc gaagcaaaac cgttgccgaa gaatgccagc gccatcaggc acataatcat
    24301 catcggatcg ttagtgtagt tagcacccat aatgcaggtg gagatcagca agccgcagat
    24361 aatcggcgtt ttacgcgcaa agcccagtga aaagccctta cgtaccagca gatccgcgac
    24421 ccagccagag agcaggacgc cgacaaacgc cgcgaggaac ggtaccgtgg tcataaagcc
    24481 cgctttcagc gccgtgatcc ctttttcctg ggttaaatag ttcgggaacc aagttaagaa
    24541 aaaccacagt gtagaagcca ccgcaaattg cccaagatag acgccgatca gtttacgatg
    24601 gaacaccagt ttccagtctt tggctgttaa cggctgacgc gcctctttct tcaccggtgc
    24661 atcgccatcc accagaccgc cgccatcacg aatgtaatcc agttcagctt tgctgatgcc
    24721 tttggtcagg cgcggcggct gataaacctt aaaccaaatc agcgaccaga taatgccgat
    24781 accaccggtg acaatgaaca cccagtgcca gctcaacagc tcctggatcc agatcagcag
    24841 cggcgtcaga aacgccagac cgacaaactg accagacgta taaaaaccaa cggcagaagc
    24901 gcgttcatgt tccgggaacc agctggtcac catccggtta ttggtcggga aagcaggcgc
    24961 ttcgaaaata ccggttatcg cgcgcagacc aattaatgac attaagcccg tggcaaagcc
    25021 ctggaataaa gtcgccactg accagccaaa tatcgcaata aaataagtca cgcgagaacc
    25081 tacgcgatct aaaaaccaac cgccggggat ctgacatagc gtataaagcc aggcgaaggc
    25141 cgaaaataca tagcccattt ccgctttggt aatgccgaac tcttcctgaa tatgggcgga
    25201 agccacggcc aggttggcgc ggtcgacata acaaatgact accgtaataa agatcatcac
    25261 cagcgtcaga taacgccgat gccccggctt tgctgcatta acgggaatat ccatagcgag
    25321 ctttctccag attttgggca tagcgaagcc gctcaccatg ccctgtaatt tacagagggt
    25381 tatatttttg tattgctgtt ttagtgcccg atgaggggct tacgtggcag gaattaccac
    25441 tctgctacgc tgttatcttc atgacgccag agcggattac gccagtccgg ggcattttta
    25501 ctgaactcaa tcactttagc ttcgtcgatt tccacgccta agcccggttt cgttaacggt
    25561 ttaaagaagc cgccgaccat gctgaagtct tctttgtttt tcacaaagtc gagtaactcc
    25621 gcgcctttgt tgtaatgaat tcccatactt tgttcctgaa gtacggcgtt ataggaaaca
    25681 aagtcgatat gcaggcaagc cgccagtgca atcggtccga gcggacagtg cggcgcaagg
    25741 gtcacgtcat aggcttctgc cattccggcg attttgtagc attcggtaat accgcccgcg
    25801 tgggagagat ccggttgcag aatcgaaata ccgcctgcct ccagcacgcg tttaaaatcg
    25861 aagcgtgaga acatgcgctc acccgccgcc agtggaatat gcgtttgtgc cgccagtttc
    25921 gggtagtatt cggcttgttc cgccagcacc ggctcctcaa taaacagcgg gcgatacggc
    25981 tccagttctt taatcagcac tttcgccatc ggcgcgctga cgcgaccgtg gaaatcaaga
    26041 ccaaactcaa tctgattgcc aaaagcttca cgaatttgtg ccacggtgtt aaccgccgca
    26101 tctaccgcgc gggagttatc aattagcccc agttcttcac aaccgttcag tttgaaggta
    26161 tcgaagccga tttcgcgtag cgttttaatg ccgtcgataa catccgccgg acgatcgccg
    26221 ccaacccaac tgtaggcttt aattttgtcg cgaaccaggc cgcccatcag ttgccagacc
    26281 ggcgcattca gcactttacc tttgatatcc cataacgcct ggtcaatccc ggcgatggcg
    26341 ctcatcagga tcggaccgcc gcgatagaat ccggcgcgat acatcacttg ccataagtca
    26401 ttgatgcgcg atggatcctg accaatcaaa tagtcaccca gctcgtgaac tgccgcttcc
    26461 accgtacggg cgcggccttc gatcacgggc tcgccccaac cgaccacgcc ttcatcggtt
    26521 tcaattttca ggaacatcca gcggggaggt aaacgatacg tggtaatttt ggtgattttc
    26581 attgcactgc ctctcgatac gccttaacaa atgctgctgc ctgctgcgcg gtgcgctcta
    26641 cggattgccc ggcgcgatag agatcgctgc ctaagcccgc ccctgcacaa cctgcgtcta
    26701 tccactgcgc caggttttct ggcgtcacgc cgccaacggc aaagactgcg atgtccgatg
    26761 gcaataccgc ttttaacgct ttgatgtatt gcggaccaaa agccgatgac ggaaatattt
    26821 tcagcgcctg cgcgcccgct tcgagcgcgg taaaggcttc ggtcgccgtc gcgcagccgg
    26881 ggcagacggt catgccgtag cccaccgcac ggcggatcac ttcactatgg atattgggcg
    26941 taacgatgag ctgacagccc atcctggcga gcgcatcgac ctgttcaggt ttcagtaccg
    27001 tacctgcgcc aatcaacgcc ttgtcgccgt acgcatcaac gatggcggga atgctttgct
    27061 cccattgtgg ggaattcagc gggatttcaa ccgcgtcgaa cccggcgtca atcaccgcgc
    27121 caacatgcgc cagcgcctcg tcgggcgtaa taccgcgcaa aatggcgatc agcgggagtt
    27181 tagtttgcca ctgcatgagc gatgctcctt ataccagcct gaaatgccgt gtcgcccgcc
    27241 accgccgtca cgtcgcaacc catcgcctga aaggcttgct ggtagcgcgc ggtcagcgat
    27301 gttccggcga caagggtgat ggcgtgttga tgggccacat agtcgcgcat actggcgacc
    27361 tctgcgccaa tcaacaaacc agagagaaat tcgctgacct gttcgcgggg aagtgttccc
    27421 agcacatgcg aggcgcgaac ttcaaaaagc tgcggcaata tggcgggcgt attaagacca
    27481 cgctcaaggc cagctgtgaa ggcatcggca gagttttcct gcggcggcaa acctgcgcca
    27541 atcaatgagt gatttaacag taaatgatgt aattcaccgg tcatcacggt gcgaaaatcg
    27601 ttgatttgct ggctatcggc ctgcacccat ttgcaatggg ttccgggcat gacataaaga
    27661 gaggaaggag ccagagctcg cgcgccgatc aattgtgttt cttcgccgcg catcacattg
    27721 tggttatcgt catgagagac acataatccg ggaataatcc agatattgtc gccaactgac
    27781 gttaattgtt cgccaataga cgaaaaacag gcaggaacag ataaatacgg tgcaactttc
    27841 cagccgacgt tgctgccaac cattcctgcc attaccactg gcgttttctc ttcacgccag
    27901 tcggtcgtga cttctgctaa caccgcagcc ggagattttc cgttcaggcg cgtgacgcct
    27961 gcttctgatt gcctgctctc caggcagtgg tcgccctgat aaagccaggc gcgcagattg
    28021 gtcgatcccc agtcaattgc gatgtagcga gctgtcatgt gatttccttt aaccttcgtg
    28081 tcgagctggc gatcatggta agcgccgcct gctctgccgc atcgccgtcc tgatgtcgta
    28141 tcgcatcgaa cagcgcctta tgttcctgga gcgtttgcgg catgttggcc tcatcgccca
    28201 tccaggttcg ttcaaaaacc gcccgctgca gcgaactgat cgcaatgcta agttgctgta
    28261 acaccgggtt atgcaccgac tgcagcaccg cctcgtggta gcgaatatcc gcttcgttaa
    28321 acgcttcgcg gtcctgattg ttggcaatca tctcgttcag cgccgattca atctgcgcca
    28381 gatcgctgga agtcgcgcgc tctgctgccc aacgggcaat cgccggttcc accagatttc
    28441 gcacttcgct catggcactg ataagccgtg ggtcgtagtc attttccagc acccattgca
    28501 gtacgtcagt gtcgaggtaa ttccactggt tacgcggtgc cacaaacgcc ccgcgataac
    28561 gtttcatttc aatcagccgc ttcgccatca gcgaacggaa cacctcacgg atgatgttgc
    28621 gcgaggttgc aaactcctca cagagttccg cctcagccgg aagcggcgag cctggcacgt
    28681 atttgccgtg aacgatctgt ttacccagcg taatgacaat gcgatcggtt ttattgagag
    28741 tcatggagag tccttgtgct ctgtatgttc ttctctactt taccccgatc gatgcataac
    28801 gcggcaactt tgtagtacca gcgtgatgac gttcgcgttt gccgtgcgtg taatgtagta
    28861 caaacttata ttgttgtact acaatttaga ccacaaaaag aacaatgcat aaaaaaatga
    28921 catgcgtcgg gcagaaatct gaaaagggat atcaggcgct aaacaggagg gaaagaagag
    28981 tatgctttca acggcttagc tactcgttta aaggattaat catgaagttg aattttaagg
    29041 gattttttaa ggctgccggt ttattcccac tggcgctgat gctttcaggc tgtatctcgt
    29101 atgctctggt ttcccatacc gcaaagggta gttcaggaaa gtatcaatcg cagtcagaca
    29161 ccatcactgg gctatcgcag gcaaaagata gtaatggaac aaaaggctat gtttttgtag
    29221 gggaatcgct ggattacctt atcactgatg gtgccgatga catcgttaag atgctcaatg
    29281 atccagcact taaccggcac aatattcagg ttgccgatga cgcaagattt gttttaaatg
    29341 cggggaaaaa gaaatttacc ggcacaatat cgctttacta ctactggaat aacgaagaag
    29401 aaaaggcact ggcaacgcat tatggttttg cctgtggtgt tcaacactgt accaggtcac
    29461 tggaaaacct aaaaggcaca atccatgaga aaaataaaaa catggattac tcaaaggtga
    29521 tggcgttcta ccatccattt aaagtgcgat tttatgaata ctattcaccc agaggcattc
    29581 cggatggtgt ttccgcagca ttactgccag tgactgttac gctggacatc attactgcac
    29641 cgctgcaatt tctggttgta tatgcagtaa accaataatc agtaagcggg caaacgcgtt
    29701 tatgctgttt gcccgcccac agattaattc agcacatact tctcaatagc aaacgccacg
    29761 ccatcttcaa ggttagattt ggtgacaaag ttcgccactt ctttcactga aggaatagcg
    29821 ttatccatcg ccacaccgac gcctgcatat tcaatcattg cgatatcgtt ttcctgatcg
    29881 ccaatcgcca tgatttcttc cggtttaata cctaacacgt cggccagtga tttcaccccc
    29941 gtacctttgt taacgcgttt atcgaggatt tcgaggaagt acggcgcact tttcagcacg
    30001 gtatatttct ctttcacttc ctgcggaata cgcgcgatag cctggtcgag gatggcgggt
    30061 tcatcaatca tcatcacttt caggaactgg gtattggggt ccattttctc cgcttcgcag
    30121 aacaccagcg gaattgtggc aacgaaggat tcatgcaccg tgtagtagct gatatcacgg
    30181 ttggcggtgt acagcgtggt gcggtccagg gcgtggaaat gagaaccgac ttcgcgagag
    30241 agtttttcca ggaaacggta gtcgtcatag ctgagagcgg tttgcgccac ggtgctacca
    30301 tcagcggcct tctgtaccag cgcgccgtta taagtaatgc agtagtcgcc cggcagttcc
    30361 atatgcagct ctttcaggta attgtgaaca cctgcataag ggcgacccgt cgttagcacg
    30421 acattcacgc cacgggcgcg agctgcggca atcgcatttt taacggcggg tgaaatggtg
    30481 tgatcgggta gcagaagggt gccatccata tcgatagcaa tgagtttgat agccatgagt
    30541 tccccaggta gattagttcc tgacccatgc taacgcgatt ccgctcaaaa atcagtacaa
    30601 cacccgaggg aaaaggggga tgcaacgcgg gttgcatgct ccccatttgc ttagcggaag
    30661 agtttccctt tcagcagttc catgcctgcg gaaagcagat cgttattggc ttgtggagac
    30721 acttcacctt gcggtgagag cgcatcaata atcttcggca attgttctgc cagtaaactg
    30781 gaagctgtac tggtatccac gccaagtttt tgtccaagat cggacaccgc atttgtgccg
    30841 agcgccgatt ccagttgctc gccactaacc gattgattgc cctgttgatt actcagccag
    30901 gttgagagaa tggcccctaa gccgccactt tgcagttttt ccagcagcac ctgaatgccg
    30961 ccctgctcct caacccaact taaaatagcc tgatatttcc ccgcatcgcc tttcagaaag
    31021 gcaccgacaa cttcatcaaa aagccccatg ataatcacct gtaaagcgtt acgtgttgac
    31081 ccaaaaagta tagatttgcg ggatgataat tgcggattgc agaaataaaa agggcggaga
    31141 tgatctccgc ccttttctta tagcttcttg ccggatgcgg cgtgaacgcc ttatccggcc
    31201 tacaaaatca tgaaaattca atacattgca agattttcgt aggcctgata agcgtagcgc
    31261 atcaggcacg ctcgcatggt tagcgccatt aaatatcgat attcgccgct ttcagggcgt
    31321 tctcttcaat aaacgcacgg cgcggttcaa cggcatcgcc catcagcgtg gtgaacaact
    31381 ggtcagcggc aatcgcatcc ttaacggtaa cgcgcagcat acgacggctt tccgggtcca
    31441 tggtggtttc ccacagctgt tccgggttca tctcgcccag acctttataa cgctggatgg
    31501 agaggccgcg acgggactct ttcaccagcc agtccagcgc ctgctcgaag ctggctaccg
    31561 gctgacgacg ctcgccacgt tcgataaacg catcttcttc cagcaagcca cgcagtttct
    31621 cacccagcgt gcagatacga cgatattcgc caccggtgat aaactcgtga tccagcggat
    31681 agtcagtatc cacaccgtgg gtacgcacgc gaacaatcgg ctcgaacagg ttttgttcgg
    31741 cattggtgtg gacatcaaac ttccactggc tgccgtgctg ttctttgtcg ttcagttcgc
    31801 tgaccagcgc gttcacccag cgggtaacgg tctgctcatc agaaaggtca gcttccgtca
    31861 acgtcggctg atagataagc tctttcagca ttgctttcgg ataacgacgc tccatacgat
    31921 tgatcatttt ctgcgtcgcg ttgtactcag ataccagttt ctctaacgct tcgccagcca
    31981 atgccggtgc actggcgttg gtgtgcagcg ttgcgccgtc cagcgcgata gagatctggt
    32041 actgatccat cgcctcgtcg tctttaatgt actgttcctg cttgcctttc ttcactttgt
    32101 acagcggcgg ctgagcgatg tagacgtgac cgcgttcaac gatttccggc atctgacgat
    32161 agaagaaggt caacagcagc gtacgaatgt gcgagccgtc gacgtccgca tcggtcatga
    32221 tgatgatgct gtgataacgc agtttgtccg ggttgtactc gtcacgaccg ataccacagc
    32281 caagcgcggt gataagcgtc gccacttcct gagaagagag catcttatcg aagcgcgctt
    32341 tctcgacgtt gaggatttta cccttcagcg gcagaatcgc ctggttcttg cggttacgcc
    32401 cctgcttcgc agagccgccc gcggagtccc cttccaccag gtacagttcg gaaagcgccg
    32461 gatcgcgttc ctggcagtct gccagtttgc ccggcaggcc cgctaagtcg agcgcacctt
    32521 tacggcgggt catttcacgc gcgcgacgcg ccgcttcacg ggcacgggca gcatcgataa
    32581 ttttgccaac cacgattttc gcgtcggttg ggttttccag caggtattct gccagcagtt
    32641 cgttcatctg ctgttcaacc gccgatttca cctcagaaga aaccagtttg tctttggtct
    32701 gggaggagaa tttcgggtcc ggcactttca cggaaacgac cgcaatcagg ccttcacgcg
    32761 catcgtcacc ggtggcgctg actttggctt ttttgctgta gccttctttg tccatgtagg
    32821 cgttcagggt acgggtcatc gccgcacgga agcctgccag gtgagtaccg ccgtcacgct
    32881 gcggaatgtt gttggtaaag cagtagatgt tttcctggaa gccatcgttc cactgcaacg
    32941 ccacttcgac gccaataccg tctttttcag tggagaagta gaagatattc gggtggatcg
    33001 gcgttttgtt cttgttcaga tattcaacga acgccttgat gccgccttca tagtggaagt
    33061 ggtcttcttt gccgtcgcgc ttgtcgcgca gacgaatgga aacgccggag ttgaggaacg
    33121 acaactcacg cagacgtttc gccagaattt catattcgaa ctcggtcaca ttggtgaagg
    33181 tttcgaggct gggccagaaa cgcaccatgg tgccggtttt ttcagtctcg ccggtaaccg
    33241 ccagcggggc ctgcggtaca ccgtgttcgt agatctgacg gtgaatttta ccctcgcgct
    33301 ggataaccag ctccagtttt tgcgacaggg cgtttactac cgaaacacca acgccgtgca
    33361 gaccgccgga cactttatag gagttatcgt caaatttacc gcctgcgtgc agaacggtca
    33421 tgatcacttc cgccgccgat acgccctctt ccgggtgaat accggtcgga atgccgcgcc
    33481 cgtcgtcctg tacagagaca gagttgtcgg catgaatggt gacgataatt tctttacagt
    33541 gacccgcgag cgcttcgtcg atagcgttat ctaccacctc gaataccatg tggtgcagac
    33601 cggtgccgtc atccgtgtcg ccgatataca tacccgggcg cttacgcacc gcatccagcc
    33661 ctttcaggac tttgatactg gaggagtcat aagaattcga catcaacgtt tctcgctcat
    33721 ttatacttgg gttaatccgt tattttaccc ttttccacgg taaacatctt cgaattttcg
    33781 tccgacatgt ctataacgtg ttcagcactg atcgcgctga caaagacctg tgattgcgtc
    33841 gcttttaagc ggctggcaag caacccgcga cgctcatcat caagctcaga ggcaaaatca
    33901 tctatcaggt agagacaccg ccgcccgctt tcacgggtga ggaactctcc ttgcgccaga
    33961 cgtaaggcgc acatcaacag cttaagctgc ccacgcgata aggtatcttc caccggcgca
    34021 ccgtcggcgc gaatgcgtaa gtctgctttg tgcgggccgt gcgcggtgta ggttagctgg
    34081 cgatcgcgtt caaaattacg ttccagcacc tcagcatatt ctgtctcttt ctcccagccg
    34141 cgctggaaag agaaagtcag agaaaactca gggagaaatt gcttacaggt atcagccata
    34201 tcagccgcga taccggcgct atactccgcg cgccaggtgc tgatttgctc cgccagcggg
    34261 atcagttctt tgtcccacgg gcgtagctgt tcgtaacgag tcacctggcg cagcgccgca
    34321 ttgcgctgct tgagcaagcg cttgagattg ctccaggcgg tgaaaaatcc aggttcgttg
    34381 tgaaagcatc cccagtcgag gaatgctctt ctgtatttgg ggccgccgtt gagtaaagta
    34441 aacccttctg gcgttatcag ctgcattggc atcaggtgcg ccagttctgc gaccttatgc
    34501 ccgtctgtac cgtcgatgcg gactttgctg tcgccctgtt tgtctttggt taagccaatc
    34561 gccgtctcgc gctcttcgcc ctgtaaacgc ccgtggagaa caaacgcctc ctgctcgtgg
    34621 cgaatgacgc gaccaatctg caaactgcga aacgcccgac cgtggccgag cgtatagatg
    34681 gcttccagca cgctggtttt gccactgccg ttggcaccta ccagaaagtt aaagccggga
    34741 gataaagcga gatccgcggt ttcgatattg cggaaatcgc ggatcaacaa gcgggtgagg
    34801 gacattacag tctcattggc atgacaacat aggcagccga ctgtgatgcg gcatcttcaa
    34861 tctgcacgct ggaaaccgaa tcggtcagca tcatgcggac gttttcgcat ttcagcgcgt
    34921 tcaggacatc cagcacataa ctgacgttga agccgatttc catctccgca ccgctatagg
    34981 taacgtcgag gatctcttcc gcttcttcct gttccgggtt attggcagtg atcttcagct
    35041 ggttttcgct gacatacagg cgcacaccgc ggaatttctc gttagagaga attgccgcgc
    35101 gagcaaacgc ctgcttgagg agatcgcaac ctgcttccag atgtttgtcc gggttcttcg
    35161 gcagaacgcg gcgataatcc gggaagcgac catccaccag tttggaggtg aagataaagt
    35221 cgccaacgtg ggcgcgaatg ttgttgctgc caatctgcac gcgcagcgga ttgtcgccgc
    35281 cgtcgagcat acgcatcagt tcaatcacgc ctttacgcgg tacgatcacc gaatggcttg
    35341 gcaaagattg accaattggc attgaacaga ctgccagacg gtgaccatcg gtcgccacgg
    35401 tgcgcagttc ttcaccttcg gtttcaaaca gcataccgtt taagtaatag cgaacatcct
    35461 gatgcgccat cgaaaactgg gtcgcttcaa tcagacgctt catcgtcgcc tgcggcaggg
    35521 taaattcgac ttcgctctgc cagtcatcga ggttcgggaa atccgccgct ggcagggtag
    35581 acagcgaaaa acggctacgc ccggagcgca ccagcatccg ttcaccttcc agctgcacgg
    35641 caatttccgc gccttcaggc agaccacggc agatatcaaa gaatttgcgc gccggaacgg
    35701 tcgtcgctcc tggctcgtgt ggctgaacca gcgcaacacg tgccaccatt tccatctcga
    35761 gatcagtacc ggtcagcgac aacgtaccgt cagcaacctg taacagcaga ttaccgagaa
    35821 tcggtagcgt aggacgacca cctaacggac cgctcacctg ttgtagcggt tttaataaat
    35881 gctcacgttc tacggtaaat ttcataggtt tacgatgaca atgttctgat taaatttgaa
    35941 aaatcttctt tgatatcgtg gctctcttca cgcagctgct cgatcttccg gcaggcatga
    36001 agcaccgtcg tgtggtcacg cccaccaaac gcatcgccaa tctctggcag actgtggtta
    36061 gtcagctctt tcgccagcgc catcgccatc tggcgcggac gcgccaccga gcgggatcga
    36121 cgcttggaaa ggagatccgc gactttgatc ttgtagtact ccgccaccgt cttctgaata
    36181 ttgtcgatgg tgaccagttt ttcctgcaat gccagcaagt cgcgcagcgc ctcacgcacg
    36241 aagtcgatgg tgatcgcccg tccggtaaag ttggcgttag caataacgcg gttcagcgcc
    36301 ccttccagct cacgtacgtt agatcgtaga cgcttggcaa taaagaacgc cacttcgcca
    36361 ggcaaacgaa tgtcattttc gtcggccttt ttcatcagga tcgccacgcg ggtttccagc
    36421 tctggcggtt cgatcgccac agtcagtccc caaccgaagc gggatttcaa acgatcctca
    36481 acgccgttga tctctttcgg atagcgatcc gaggtgagaa tgatctgttg attaccttcc
    36541 agcagggcgt tgaaggtgtg gaaaaactct tcctgagatc gttctttatt agcaaaaaac
    36601 tgaatatcgt cgatcagcag tgcatctacg gaacggtagt agcgtttaaa ctcttcgatc
    36661 gcgttgtttt gcagggcttt aaccatgtcc tgaacaaagc gctcggagtg catataaacc
    36721 actttggcat tcggcttgcg cgccataatg ccgttaccca ccgcatgcag caggtgagtt
    36781 ttacccagac ccgtgccgcc ataaaggaac aacgggttat aggcaccgcc agggttatcc
    36841 gccacctggc gagccgccgc gcgcgccagt tggttagatt taccttcgac gaagttatca
    36901 aacgtgtgtt tgacgtttac gttagaacga taggtcggtt ctgccggggc cgggacgtta
    36961 tcccaacctg agcgcataga aggcgcagca cgttgcggct gcgtttgcgc cacctgtgca
    37021 ggggccgcga cgttgctcgt cactgccgct tgtggcgttt gcgtcaccgg tttggtgccg
    37081 acttcaaaac gcagctgtgg ggcatccgct ccgcagaaac tggttagcag tccattgata
    37141 ttattaaggt acttgtcccg tacccaatcg aggacaaaac ggtttggcgc gtacagggcc
    37201 agcgtgttat cgctcagttc cgcctgcaat gggcgtatcc acatactgaa ttctgtggct
    37261 ggtaactcat cctgcaatcg ggcaagacac tgctgccaaa gcgaaagtga cacggcggac
    37321 tccactcgaa caaaagtcga taatgactaa ggctgaaaca ttcatgattg ttgacgtacg
    37381 tcgaaaagac cctgcttgag ggtgacgcac gaaccgctgt ctgcggttat atgcccgatc
    37441 aagatcctgc aaaacgatcg ggaccgcgga tcatagccta aactgcgcaa gagatcttct
    37501 gtttctcaca gattttcccg atttatccac aggatgatcc agaactcgct aagtgtaaac
    37561 gatcctgccg caaggcgggc acgatttacg ccgcatattg gaaaatttaa tgaccataga
    37621 caaaaattgg cttaatcgat ctaataaaga tccaggacga tccttgcgct ttacccatca
    37681 gcccgtataa tcctccaccc ggcgcgccat gctggttttc actggtgtga ggtcgtacat
    37741 tttccctacg aaaaggtgcg gaaaagcgcg gtaaataagg aaagagaatt gactccggag
    37801 tgtacaatta ttacaatccg gcctctttaa tcacccatgg cttcggtgtc catcgtttca
    37861 tttttcggcg gatatccaat aaagccattg aatttattca agtttaggta gaaatcgcca
    37921 tgaaacgcac ttttcaaccg tctgtactga agcgcaaccg ttctcacggc ttccgtgctc
    37981 gtatggctac taaaaatggt cgtcaggttc tggcacgtcg tcgtgctaaa ggccgcgctc
    38041 gtctgaccgt ttctaagtaa taaagctaac ccctgagtgg ttaagctcgc atttcccagg
    38101 gagttacgct tgttaactcc cagtcaattc acattcgtct tccagcagcc acaacgggct
    38161 ggcacgccgc aaattaccat tctcggccgc ctgaattcgc tggggcatcc ccgtatcggt
    38221 cttacaatcg ccaagaaaaa cgttcgacgc gcccatgaac gcaatcggat taaacgtctg
    38281 acgcgtgaaa gcttccgtct gcgccaacat gaactcccgg ctatggattt cgtggtggtg
    38341 gcgaaaaaag gggttgccga cctcgataac cgtgctctct cggaagcgtt ggaaaaatta
    38401 tggcgccgcc actgtcgcct ggctcgcggg tcctgatagc cctcattcgg gtctatcaac
    38461 gcctgattag tccgctactc gggccgcatt gtcgtttcac tccaacctgt tcaagctacg
    38521 gaattgaggc attgcgcagg tttggagtga taaaaggcag ttggttgacg gtgaaacgcg
    38581 tattaaaatg ccacccttta caccctggtg gtgacgatcc cgtcccgccc ggaccatttg
    38641 ataccagaga acactaacga tggattcgca acgcaatctt ttagtcatcg ctttgctgtt
    38701 cgtgtctttc atgatctggc aagcctggga gcaggataaa aacccgcaac ctcaggccca
    38761 acagaccacg cagacaacga ccaccgcagc gggtagcgcc gccgaccagg gcgtaccggc
    38821 cagtggccag gggaaactga tctcggttaa gaccgacgtg cttgatctga ccatcaacac
    38881 ccgtggtggt gatgttgagc aagctctgct gcctgcttac ccgaaagagc tgaactctac
    38941 ccagccgttc cagctgctgg aaacttcacc gcagtttatt tatcaggcac agagcggtct
    39001 gaccggtcgt gatggcccgg ataacccggc taacggcccg cgtccgctgt ataacgttga
    39061 aaaagacgct tatgtgctgg ctgaaggtca aaacgaactg caggtgccga tgacgtatac
    39121 cgacgcggca ggcaacacgt ttaccaaaac gtttgtcctg aaacgtggtg attacgctgt
    39181 caacgtcaac tacaacgtgc agaacgctgg cgagaaaccg ctggaaatct ccacctttgg
    39241 tcagttgaag caatccatca ctctgccacc gcatctcgat accggaagca gcaacttcgc
    39301 actgcacacc ttccgcggcg cggcgtactc cacgcctgac gagaagtacg agaaatacaa
    39361 gttcgatacc attgccgata acgaaaacct gaacatctct tcgaaaggtg gttgggtggc
    39421 aatgctgcaa cagtatttcg cgacggcgtg gatcccgcat aacgacggta ccaacaactt
    39481 ctataccgct aatctgggta acggcatcgc cgctatcggc tataaatctc agccggtact
    39541 ggttcagcct ggtcagactg gcgcgatgaa cagcaccctg tgggttggcc cggaaatcca
    39601 ggacaaaatg gcagctgttg ctccgcacct ggatctgacc gttgattacg gttggttgtg
    39661 gttcatctct cagccgctgt tcaaactgct gaaatggatc catagctttg tgggtaactg
    39721 gggcttctcc attatcatca tcacctttat cgttcgtggc atcatgtacc cgctgaccaa
    39781 agcgcagtac acctccatgg cgaagatgcg tatgttgcag ccgaagattc aggcaatgcg
    39841 tgagcgtctg ggcgatgaca aacagcgtat cagccaggaa atgatggcgc tgtacaaagc
    39901 tgagaaggtt aacccgctgg gcggctgctt cccgctgctg atccagatgc caatcttcct
    39961 ggcgttgtac tacatgctga tgggttccgt tgaactgcgt caggcaccgt ttgcactgtg
    40021 gatccacgac ctgtcggcac aggacccgta ctacatcctg ccgatcctga tgggcgtaac
    40081 gatgttcttc attcagaaga tgtcgccgac cacagtgacc gacccgatgc agcagaagat
    40141 catgaccttt atgccggtca tcttcaccgt gttcttcctg tggttcccgt caggtctggt
    40201 gctgtactat atcgtcagca acctggtaac cattattcag cagcagctga tttaccgtgg
    40261 tctggaaaaa cgtggcctgc atagccgcga gaagaaaaaa tcctgattcg gtgagttttc
    40321 gctaaaataa gggcggtcag ttgaccgcct tttttctttt cgtagggcgg ataagcaccg
    40381 cgcatccgcc acacaaagca acaggaacat catgagcgat aatgacacta tcgtagccca
    40441 ggccacgcct ccgggacgtg gcggcgttgg catcctgcgc atctccggct tcaaagcccg
    40501 tgaagttgcc gaaaccgtgc tgggtaaact gcctaagccg cgctacgccg attatcttcc
    40561 gtttaaagac gccgacggca gcgtgctcga tcaggggatt gcgctatggt tccctggccc
    40621 gaactcgttc accggcgaag atgtgctgga actgcaaggt catggcggtc cggtgatcct
    40681 cgacctgctg ttaaaacgca ttctgaccat tcccggcctg cggattgctc gccctggtga
    40741 gttttccgaa cgcgcgtttc ttaacgataa acttgactta gcccaggccg aggcgattgc
    40801 cgatcttatc gacgccagtt cggaacaggc ggcccgttcg gcacttaact cgctgcaagg
    40861 cgcattctcc gcacgggtta atcatctggt agaagccctc acccacttgc gcatttacgt
    40921 cgaagcggca attgatttcc ccgatgaaga gatcgatttc ctctccgacg gaaaaattga
    40981 agcccagctc aatgacgtta ttgccgatct tgatgcagtg cgtgctgaag cacgtcaggg
    41041 tagtttgttg cgcgaaggga tgaaagtggt gattgccgga cgtcctaacg ccggtaaatc
    41101 gagcctgtta aacgcgctgg cggggcgtga agcggcaatc gtaaccgata tcgccggaac
    41161 tacgcgtgac gtgctgcgtg agcatatcca cattgacgga atgccgctgc atatcatcga
    41221 taccgccggg ctacgtgaag ccagtgacga agtagaacgt attggtatcg agcgcgcgtg
    41281 gcaggaaatt gaacaggccg accgcgtgct gtttatggtc gatggcacca caacagacgc
    41341 cgtggatccg gcagagatct ggccggaatt tattgcccgt ctgccagcga aactgccgat
    41401 caccgtggtg cgcaataaag ccgatatcac cggcgaaacg ctgggaatga gtgaagtgaa
    41461 cggtcacgcg ttaattcgtc tctcggcaag gactggtgaa ggcgtggacg tgctgcgtaa
    41521 ccatctcaaa cagagcatgg gctttgacac caacatggaa ggcggcttcc tggcgcgtcg
    41581 tcgccaccta caggcgctgg aacaggcagc ggaacatcta caacagggca aagcgcaact
    41641 gttgggagcc tgggcaggtg aactgctggc ggaagagttg cgtctggcac agcagaactt
    41701 aagcgaaatc accggggaat ttacttcaga cgacctgctg gggcggattt tctccagctt
    41761 ctgtattggt aagtaaccgc gcttacgaag ccgcattctg actgtcagat gcggcttcgc
    41821 ttcattgtta ccactcctgt tattcctcaa cccttttttt aaacattaaa attcttacg
//
LOCUS       DAPHBJ010000015        41525 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.015, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000015 DAPHBJ010000000
VERSION     DAPHBJ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.015"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            5..1657
                     /gene="hcp"
                     /locus_tag="RHZ76_000772"
                     /gene_synonym="priS"
     CDS             5..1657
                     /gene="hcp"
                     /locus_tag="RHZ76_000772"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDV2811464.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            1669..2637
                     /gene="hcr"
                     /locus_tag="RHZ76_000773"
     CDS             1669..2637
                     /gene="hcr"
                     /locus_tag="RHZ76_000773"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HDV2811465.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNCPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            2770..4488
                     /gene="poxB"
                     /locus_tag="RHZ76_000774"
     CDS             2770..4488
                     /gene="poxB"
                     /locus_tag="RHZ76_000774"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HDV2811466.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATMHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            4525..5526
                     /gene="ltaE"
                     /locus_tag="RHZ76_000775"
     CDS             4525..5526
                     /gene="ltaE"
                     /locus_tag="RHZ76_000775"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDV2811467.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            5537..6967
                     /locus_tag="RHZ76_000776"
     CDS             5537..6967
                     /locus_tag="RHZ76_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HDV2811468.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRVLMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            7066..8079
                     /locus_tag="RHZ76_000777"
     CDS             7066..8079
                     /locus_tag="RHZ76_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDV2811469.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(8076..8906)
                     /gene="amiD"
                     /locus_tag="RHZ76_000778"
     CDS             complement(8076..8906)
                     /gene="amiD"
                     /locus_tag="RHZ76_000778"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HDV2811470.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTTSL
                     LELLARYGYDVKPDMIPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(8903..9226)
                     /locus_tag="RHZ76_000779"
     CDS             complement(8903..9226)
                     /locus_tag="RHZ76_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDV2811471.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            9352..9867
                     /locus_tag="RHZ76_000780"
     CDS             9352..9867
                     /locus_tag="RHZ76_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV2811472.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            10085..10813
                     /gene="artP"
                     /locus_tag="RHZ76_000781"
     CDS             10085..10813
                     /gene="artP"
                     /locus_tag="RHZ76_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HDV2811473.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            10831..11562
                     /gene="artJ"
                     /locus_tag="RHZ76_000782"
     CDS             10831..11562
                     /gene="artJ"
                     /locus_tag="RHZ76_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV2811474.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            11569..12285
                     /gene="artQ"
                     /locus_tag="RHZ76_000783"
     CDS             11569..12285
                     /gene="artQ"
                     /locus_tag="RHZ76_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HDV2811475.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            12285..12953
                     /gene="artM"
                     /locus_tag="RHZ76_000784"
     CDS             12285..12953
                     /gene="artM"
                     /locus_tag="RHZ76_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HDV2811476.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            13179..13910
                     /gene="artJ"
                     /locus_tag="RHZ76_000785"
     CDS             13179..13910
                     /gene="artJ"
                     /locus_tag="RHZ76_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HDV2811477.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(14109..15236)
                     /gene="rlmC"
                     /locus_tag="RHZ76_000786"
     CDS             complement(14109..15236)
                     /gene="rlmC"
                     /locus_tag="RHZ76_000786"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HDV2811478.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGLEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWVRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDVRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(15277..15765)
                     /locus_tag="RHZ76_000787"
     CDS             complement(15277..15765)
                     /locus_tag="RHZ76_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HDV2811479.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFL
                     LQ"
     gene            complement(15825..16670)
                     /gene="potI"
                     /locus_tag="RHZ76_000788"
     CDS             complement(15825..16670)
                     /gene="potI"
                     /locus_tag="RHZ76_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HDV2811480.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDSSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(16667..17620)
                     /gene="potH"
                     /locus_tag="RHZ76_000789"
     CDS             complement(16667..17620)
                     /gene="potH"
                     /locus_tag="RHZ76_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HDV2811481.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(17630..18763)
                     /gene="potG"
                     /locus_tag="RHZ76_000790"
     CDS             complement(17630..18763)
                     /gene="potG"
                     /locus_tag="RHZ76_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HDV2811482.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(18858..19970)
                     /gene="potF"
                     /locus_tag="RHZ76_000791"
     CDS             complement(18858..19970)
                     /gene="potF"
                     /locus_tag="RHZ76_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HDV2811483.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(20321..20797)
                     /locus_tag="RHZ76_000792"
     CDS             complement(20321..20797)
                     /locus_tag="RHZ76_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HDV2811484.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(20885..21787)
                     /gene="rimK"
                     /locus_tag="RHZ76_000793"
     CDS             complement(20885..21787)
                     /gene="rimK"
                     /locus_tag="RHZ76_000793"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HDV2811485.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIGMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(21848..22570)
                     /gene="nfsA"
                     /locus_tag="RHZ76_000794"
     CDS             complement(21848..22570)
                     /gene="nfsA"
                     /locus_tag="RHZ76_000794"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HDV2811486.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPSSILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(22554..22841)
                     /locus_tag="RHZ76_000795"
     CDS             complement(22554..22841)
                     /locus_tag="RHZ76_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HDV2811487.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            23001..23258
                     /gene="grxA"
                     /locus_tag="RHZ76_000796"
     CDS             23001..23258
                     /gene="grxA"
                     /locus_tag="RHZ76_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HDV2811488.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNEHDDFQYHYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(23288..23665)
                     /locus_tag="RHZ76_000797"
     CDS             complement(23288..23665)
                     /locus_tag="RHZ76_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HDV2811489.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            23935..25620
                     /locus_tag="RHZ76_000798"
     CDS             23935..25620
                     /locus_tag="RHZ76_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HDV2811490.1"
                     /translation="MNINVAELLNRNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(25795..26331)
                     /gene="rcdA"
                     /locus_tag="RHZ76_000799"
     CDS             complement(25795..26331)
                     /gene="rcdA"
                     /locus_tag="RHZ76_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HDV2811491.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            26415..27623
                     /locus_tag="RHZ76_000800"
     CDS             26415..27623
                     /locus_tag="RHZ76_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2811492.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGMGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKVVAKPD
                     TKTQTAMENS"
     gene            27623..28438
                     /gene="ybjI"
                     /locus_tag="RHZ76_000801"
     CDS             27623..28438
                     /gene="ybjI"
                     /locus_tag="RHZ76_000801"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HDV2811493.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            28524..28808
                     /locus_tag="RHZ76_000802"
     CDS             28524..28808
                     /locus_tag="RHZ76_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2811494.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(28849..30081)
                     /gene="mdfA"
                     /locus_tag="RHZ76_000803"
     CDS             complement(28849..30081)
                     /gene="mdfA"
                     /locus_tag="RHZ76_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HDV2811495.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGQFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            30366..30962
                     /gene="ybjG"
                     /locus_tag="RHZ76_000804"
     CDS             30366..30962
                     /gene="ybjG"
                     /locus_tag="RHZ76_000804"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDV2811496.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAVV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            31020..31778
                     /gene="deoR"
                     /locus_tag="RHZ76_000805"
     CDS             31020..31778
                     /gene="deoR"
                     /locus_tag="RHZ76_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HDV2811497.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(31825..33027)
                     /gene="dacC"
                     /locus_tag="RHZ76_000806"
     CDS             complement(31825..33027)
                     /gene="dacC"
                     /locus_tag="RHZ76_000806"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDV2811498.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRVWDFVMMKFH
                     QWFGSWFS"
     gene            33274..33900
                     /gene="gstB"
                     /locus_tag="RHZ76_000807"
     CDS             33274..33900
                     /gene="gstB"
                     /locus_tag="RHZ76_000807"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HDV2811499.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(33897..35012)
                     /gene="yliI"
                     /locus_tag="RHZ76_000808"
     CDS             complement(33897..35012)
                     /gene="yliI"
                     /locus_tag="RHZ76_000808"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HDV2811500.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(35123..35506)
                     /gene="bssR"
                     /locus_tag="RHZ76_000809"
     CDS             complement(35123..35506)
                     /gene="bssR"
                     /locus_tag="RHZ76_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HDV2811501.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            35719..37044
                     /gene="rimO"
                     /locus_tag="RHZ76_000810"
     CDS             35719..37044
                     /gene="rimO"
                     /locus_tag="RHZ76_000810"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDV2811502.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            37270..37656
                     /locus_tag="RHZ76_000811"
     CDS             37270..37656
                     /locus_tag="RHZ76_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV2811503.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSGLSSKGTKEIEDDELAAYKKMAN
                     AFLAFSNKQIEDLIETGFLIEVKNER"
     gene            37646..37975
                     /locus_tag="RHZ76_000812"
     CDS             37646..37975
                     /locus_tag="RHZ76_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDV2811504.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(38045..39373)
                     /locus_tag="RHZ76_000813"
     CDS             complement(38045..39373)
                     /locus_tag="RHZ76_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDV2811505.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYGLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEFNAKNTIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(39381..>41525)
                     /locus_tag="RHZ76_000814"
     CDS             complement(39381..>41525)
                     /locus_tag="RHZ76_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HDV2811506.1"
                     /translation="KDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSR
                     RANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRP
                     GKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAK
                     NQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPGGNLLNASDKL
                     FYLPFAFIIIVLLVVYLMTTRVFRRQFSEMTELVNTLAFLPDSTDQIEALKIREGDAK
                     EIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLC
                     GLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKI
                     AMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDD
                     AKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSAERGIIS
                     PLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINISPWQLNEPHF
                     HERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLS
                     NLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARE
                     LIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT        10330 a        10769 c        10314 g        10112 t
ORIGIN      
        1 gatcatgttt tgtgtgcaat gtgaacaaac tatccgtact ccggcaggaa acggctgctc
       61 atacgcgcag gggatgtgtg gtaaaacggc ggaaacttct gaccttcagg atttactcat
      121 cgcggcgctg caagggcttt ctgcctgggc ggtaaaagcg cgtgaatacg gcatcatcaa
      181 ccacgatgtt gacagcttcg cgccacgtgc ctttttctca accctgacca acgttaactt
      241 cgattctccg cgtattgtcg gctacgctcg tgaagcgatt gccctgcgcg aggcgctgaa
      301 agcgcaatgc ctggctgtag atgccaacgc gcgcgtcgat aacccgatgg ctgacctgca
      361 actggtgagc gacgatctcg gcgagctgca acgtcaggca gcagaattta cccctaacaa
      421 agataaagcg gcgattggcg aaaacattct cggcctgcgt ctgctgtgcc tgtatggcct
      481 gaaaggtgcg gcggcctata tggaacacgc gcatgttctc ggtcaatacg acaacgatat
      541 ttatgcccag tatcataaaa tcatggcgtg gctggggacc tggcctgccg atatgaacgc
      601 gcttcttgag tgttcaatgg aaatcggcca gatgaacttc aaagtgatga gcattctgga
      661 tgcaggcgaa accggtaaat acggtcaccc gacgccaacc caggtcaacg tcaaagcgac
      721 ggcgggtaaa tgcatcctga tttccggtca cgatctcaaa gatctctaca acctgctgga
      781 acagaccgaa ggcacgggcg ttaatgtcta cacccacggc gaaatgctgc ctgcgcatgg
      841 ctacccggag ctgcgtaaat tcaagcatct ggtcggtaac tacggcagcg gctggcagaa
      901 tcagcaagtg gagttcgctc gtttccctgg ccccatcgtg atgacctcga actgcatcat
      961 cgacccaacc gtaggcgctt atgacgatcg tatctggacc cgcagcattg ttggctggcc
     1021 tggcgtgcgt catctggacg gtgatgattt ctctgcggtt atcacccagg cgcaacagat
     1081 ggcaggcttc ccgtacagcg aaattccgca ccttatcacc gtgggttttg gtcgccagac
     1141 gctgcttggc gctgctgata cgctgattga tctggtgagc cgtgaaaaac tgcgtcatat
     1201 cttcctgctt ggtggctgtg acggcgcacg cggcgagcgc cactacttca ccgatttcgc
     1261 caccagcgtg ccggatgact gcctgatcct gaccctcgcc tgtggtaaat atcgctttaa
     1321 caaacttgag tttggcgata tcgaaggtct gccgcgtctg gtagatgcag gtcaatgtaa
     1381 cgatgcttac tcagcgatta ttctggctgt cactctggca gagaaactgg gctgtggtgt
     1441 gaacgatctg ccgctgtcgc tggtgctctc ctggtttgaa cagaaagcga tcgtcattct
     1501 gctgacgctg ctttctctgg gcgtgaaaaa tatcgtcacc ggcccgactg cacctggttt
     1561 cctgacacca gacctgctgg cggtgctgaa cgagaaattc ggcctgcgtt ctatcactac
     1621 tgttgaagaa gacatgaagc aactgttgag cgcgtaagga gtttgtcgat gacgatgcca
     1681 acgaatcaat gcccgtggcg gatgcaggtt catcacatta cgcaagaaac gccggatgtg
     1741 tggacgattt ccctgatttg ccacgattac tacccatatc gcgccgggca atatgcactg
     1801 gtcagcgtgc gaaactcagc ggaaacgctg cgtgcttaca ccatttcctc cacgccaggc
     1861 gtgagtgaat atatcaccct gaccgtgcgg cggattgatg acggtgtcgg ctcccagtgg
     1921 ctgacgcgcg atgtaaaacg cggtgattat ctctggcttt cggacgcgat gggggaattt
     1981 acctgcgacg ataaagcaga agataaattc ctgttgctgg cggcaggctg cggcgtcacg
     2041 ccgattatgt cgatgcgtcg ctggctggcg aagaactgtc cacaggccga tgtacaggtg
     2101 atctacaacg tgcgcacgcc gcaggatgtt attttcgccg atgagtggcg taactatccg
     2161 gtaacgctgg tggcggaaaa taacgttacc gaaggcttta tcgctggtcg tctcactcgc
     2221 gaactgctga ctcgcgttcc tgacttagct tcacgtaccg tgatgacctg cggcccggct
     2281 ccgtatatgg attgggtaga gcaggaagtg aaagcgctcg gcgtgacgcg tttctttaaa
     2341 gagaaattct tcaccccagt agcggaagca gcgaccagcg gtctgaaatt caccaaactg
     2401 caaccggcac gagaatttta cgccccggtt ggcaccacgc tactggaggc gctggaaagc
     2461 aataacgttc cggttgtcgc cgcctgccgt gcgggtgttt gcggctgctg taagacaaaa
     2521 gtggtttccg gtgaatatac ggtgagcagc acaatgacgc tgaccgacgc cgaaatcgct
     2581 gaaggttacg tactggcctg ctcctgccat ccgcaggggg atttggttct cgcataatcg
     2641 ccttatgccc gatgatattc ctttcatcgg gctatttaac cgttagtgcc tcctttctct
     2701 cccatccctt ccccctccgt cagatgaact aaacttgtta ccgttatcac attcaggaga
     2761 tggagaacca tgaaacaaac ggttgcagct tatatcgcca aaacactcga atcggcaggg
     2821 gtgaaacgca tctggggagt cacaggcgac tctctgaacg gtcttagtga cagtcttaat
     2881 cgcatgggca ccatcgagtg gatgtccacc cgccacgaag aagtggcggc ctttgccgct
     2941 ggcgctgaag cacaacttag cggagaactg gcggtctgcg ccggatcgtg cggccccggc
     3001 aacctgcact taatcaacgg cctgttcgat tgccaccgca atcacgttcc ggtactggcg
     3061 attgccgctc atattccctc cagcgaaatt ggcagcggct atttccagga aacccaccca
     3121 caagagctat tccgcgaatg tagtcactat tgcgagctgg tttccagccc ggagcagatc
     3181 ccacaagtgc tggcgattgc catgcgcaaa gcggtgctta accgtggcgt ttcggttgtc
     3241 gtgttaccag gcgacgtggc gttaaaacct gcgccagaag gggcaaccat gcactggtat
     3301 catgcgccac aaccagtcgt gacgccggaa gaagaagagt tacgcaaact ggcgcaactg
     3361 ctgcgttatt ccagcaatat cgccctgatg tgtggcagcg gctgcgcggg ggcgcataaa
     3421 gagttagttg agtttgccgg gaaaattaaa gcgcctattg ttcatgccct gcgcggtaaa
     3481 gaacatgtcg aatacgataa tccgtatgat gttggaatga ccgggttaat cggcttctcg
     3541 tcaggtttcc ataccatgat gaacgccgac acgttagtgc tactcggcac gcaatttccc
     3601 taccgcgcct tctacccgac cgatgccaaa atcattcaga ttgatatcaa cccagccagc
     3661 atcggcgctc acagcaaggt ggatatggca ctggtcggcg atatcaagtc gactctgcgt
     3721 gcattgcttc cattggtgga agaaaaagcc gatcgcaagt ttctggataa agcgctggaa
     3781 gattaccgcg acgcccgcaa agggctggac gatttagcta aaccgagcga gaaagccatt
     3841 cacccgcaat atctggcgca gcaaattagt cattttgccg ccgatgacgc tattttcacc
     3901 tgtgacgttg gtacgccaac ggtgtgggcg gcacgttatc taaaaatgaa cggcaagcgt
     3961 cgcctgttag gttcgtttaa ccacggttcg atggctaacg ccatgccgca ggcgctgggt
     4021 gcgcaggcga cagagccaga acgtcaggtg gtcgccatgt gcggcgatgg cggttttagc
     4081 atgttgatgg gcgatttcct ctcagtagtg cagatgaaac tgccagtgaa aattgtcgtc
     4141 tttaacaaca gcgtgctggg ctttgtggcg atggagatga aagctggtgg ctatttgact
     4201 gacggcaccg aactacacga cacaaacttt gcccgcattg ccgaagcgtg cggcattacg
     4261 ggtatccgtg tagaaaaagc gtctgaagtt gatgaagccc tgcaacgcgc cttctccatc
     4321 gacggtccgg tgctggtgga tgtggtggtc gccaaagaag agttagccat tccaccgcag
     4381 atcaaactcg aacaggccaa aggatttagc ttgtatatgt tgcgcgcaat catcagcgga
     4441 cgcggcgatg aagtgatcga actggcgaaa acgaactggc taaggtaaaa agggtggcat
     4501 ttcccgtcat aataaggaca tgccatgatt gatttacgca gtgataccgt tacccgaccg
     4561 agccgcgcca tgctcgaagc aatgatggcc gccccggttg gggacgacgt ttacggagac
     4621 gaccctaccg ttaatgctct gcaggactac gccgcagagc tttccggtaa agaagccgcc
     4681 atttttctgc ctaccggcac tcaggccaac ctggtcgctc tgctcagtca ctgcgaacgt
     4741 ggcgaagagt atattgtcgg tcaggccgcg cataactatc tgtttgaagc cggtggcgca
     4801 gcggtgctgg gcagtattca gccgcaaccc atcgacgcgg ctgccgacgg cacgctaccg
     4861 ctggataaag tggcgatgaa aatcaaaccc gacgatatcc atttcgcccg caccaaatta
     4921 ctcagtctgg aaaacaccca caacggcaaa gtgctgccgc gtgaatacct gaaagaagca
     4981 tgggaattta cccgcgagcg caatctggcg ctgcatgttg acggtgcgcg catctttaat
     5041 gccgtggtgg cttacggctg cgaactgaaa gagatcacgc aatattgtga ttcgttcacc
     5101 atttgcctgt cgaaaggtct tgggacgcca gtcggttcat tactcgtcgg taatcgtgat
     5161 tacattaaac gtgccatccg ctggcggaaa atggccggtg gcgggatgcg tcagtccggg
     5221 attctggctg ccgccgggat gtatgccctg aaaaataacg tcgcacggtt gcaggaagat
     5281 cacgacaacg ccgcctggat ggcggagcaa ctgcgtgaag caggcgcgga tgtgatgcgt
     5341 caggacacta atatgctgtt tgttcgcgtc ggggaagaaa atgctgccgc gttaggcgaa
     5401 tacatgaaag cgagaaacgt gctgattaac gcctcgccga ttgtccgcct ggtgacgcat
     5461 cttgacgtct cgcgcgaaca actggcggaa gtcgccgccc actggcgcgc attcctggcg
     5521 cgttaaggag agagacgtgc cgcaacgcat tttagttctc ggtgccagtg gctacattgg
     5581 tcagcatctg gtgcgcacac tcagccagca agggcatcag atcctggcgg cggcacgtca
     5641 tgtcgacagg cttgcaaagc tgcaactggc aaacgtcagt tgccataaag tcgatctcag
     5701 ctggccggat aaccttccgg ccctgttgca ggatatcgat accgtctatt ttctggtgca
     5761 cagcatgggc gaaggcggcg attttatcgc tcaggagcgc caggtggctc tcaacgtccg
     5821 cgatgcgcta cgtgaagtac cagttaagca attaatcttt ctcagttcgt tgcaggcccc
     5881 gccacatgag cagtcggatc atctgcgtgc tcgtcaggct acggcggaca ttcttcgtga
     5941 agcgaatgta cctgtgaccg aacttcgggc cggaattatc gttggcgcag gttcagcggc
     6001 gttcgaagtc atgcgcgata tggtctacaa cctgccagtg ttaacgccgc cacgctgggt
     6061 acgttcacgc accacgccca tcgcgctgga aaacttgctg cactatctgg tggcgttgtt
     6121 agatcatccg gccagcgaac accgcatctt cgaagccgcc ggaccagagg tgctcagtta
     6181 tcagcaacag tttgaacatt ttatggcggt gagcggtaag cgccgctggt tgatccccat
     6241 ccccctcccc acccgctgga tttcggtgtg gtttctcaat gtgattactt ccgtaccgcc
     6301 caccaccgcc agggcgttga ttcaggggct gaaacacgat ctgctggcgg atgataccgc
     6361 gctacgtgca ctcatcccac aacggctgat cgctttcgat gacgcggtac gtagcacgtt
     6421 gaaagaggag gaaaaactgg tcaactccag cgactggggc tacgacgctc aggcctttgc
     6481 ccgctggcga ccggagtacg gttattttgc caaacaggcg gggtttaccg ttaaaacgtc
     6541 cgccagcctt gctgctttat ggcaggtagt gaaccaaatc ggcggtaaag agcgttattt
     6601 ctttggcaat attttgtggc agacacgggt gttgatggac cgcgcgatcg gtcataagct
     6661 ggcgaaaggc cgcccggagc gcgaatattt acagactggc gatgcggtag atagctggaa
     6721 agtgattgtc gttgaaccgg aaaaacaact tacgttgtta tttggcatga aagcgccggg
     6781 gctgggacga ctgtgtttta gcctggaaga taaaggcgac tatcgtacta tcgatgtccg
     6841 cgctttctgg catccgcacg gtatgccggg gctgttttac tggttattga tgatccccgc
     6901 gcatctgttt atttttcgcg gaatggcaaa acaaatcgcc agactggcag aacaaagcac
     6961 agattaatcg acaaaacctg cgttattctt tcatgtttcc cattgcatca tgggggaaat
     7021 cacggaagaa tgcgcacgtt attttctcaa caacagtgga ttgatatgaa ggtactggtt
     7081 accggcgcca ccagcggctt aggtcgaaac gcggtagagt ttttatgcca gaaaggcatc
     7141 agcgtgcgag cgaccggtcg caacgaggca atgggcaaat tgctggaaaa aatgggcgca
     7201 gagtttgttc cggcggatct gaccgagctg gtttcatcac aagctaaagt gatgctcgcg
     7261 ggcattgata cgctgtggca ctgctccagc tttacctcac cctgggggac acaacaggct
     7321 ttcgatctgg ctaacgttcg cgccactcgc cgcctgggtg aatgggctgt cgcctggggt
     7381 gtgcgtaact ttattcatat ctcttccccc tccctgtact tcgattatca ccaccatcgc
     7441 gatattaaag aagattttcg ccctcaccgc ttcgccaacg agtttgcccg cagcaaagca
     7501 gccagcgaag aagtgatcaa tatgctttcg caggcgaatc cacaaacgcg ctttactatt
     7561 ctgcgcccac aaagtctgtt cggaccgcac gataaagtct ttattccccg tctggcgcat
     7621 atgatgcacc actacggcag tattctgtta ccgcatggcg gcagtgcgct ggtcgatatg
     7681 acctactatg aaaatgccgt gcacgcaatg tggctggcaa gccaggaagc ctgcgataag
     7741 ctaccttccg ggcgtgtgta caacatcacc aacggcgagc atcgcacact gcgcagcatc
     7801 gtgcagaagc tgatcgacga gttgaatatt gactgtcgta ttcgttccgt cccctacccg
     7861 atgctggata tgatcgcccg cagcatggag cgtttaggcc gcaagtcagc aaaagagccg
     7921 ccgctgaccc actacggcgt ctccaagttg aattttgact ttacgctgga tattacgcgt
     7981 gcgcaggaag agttaggtta tcagccggtc atcaccctgg atgaaggtat cgagaaaact
     8041 gccgcctggc tacgcgacca cggaaaactg ccgcgctaat cctgcccgta tttctccagc
     8101 aatgcttcgg caatcgcctg agtttctgca tccgcttcgc cgttatataa cgtcgggcgg
     8161 aaatgcatct ggaatgccat aatcacgcgc cgctgctcgc gcggtatcat atcaggttta
     8221 acgtcataac cgtagcgcgc caaaagctcc agcaatgacg tagtatctac aggagtgtgc
     8281 ggcgcgcgcc cggcaaggta aaagttaacc cgctgcgcat ccggccaggc accaatcccc
     8341 tgctgcgcca gttgctgcca gggaaataat ggccccggat cgtctttgcg ctgcggtgcg
     8401 atatccgcat gtgccactac gttttccggc ttgatgtgat aacgggcaat aatatctttc
     8461 gccagtggaa taagcgcctg aatctgtgcc ggttcaaacg gggcaaaata tttcactccg
     8521 gccgattttt gccagccacg gttttccagc tcaatgccaa tagaggtgtc gttaaggcgc
     8581 gttgccccgc gccaggcgct aatccccgca tgccaggcca gttcttgttc cggcaccagt
     8641 tgccagatgc gcggtttacc gttgtatcgc ggtggtaccg cagggaccag ataatgcgag
     8701 ctgacctgct tatcggtcag tgtcgccagc gagctatcaa aatcatctgc ggtgtagtgg
     8761 atcaccagca ctttaatgcg cggatacgcc gcctgcgcct ggcgtcgggt atcaagctga
     8821 tatccctctt tctcgacaat gcctttttcg cctgcacacc ctgccaataa cagagcggca
     8881 gcgaccagcc agaagactct tctcatcgac gcgttttcac cgccgtaccg ctgacgctaa
     8941 ccatcagcat actgccgttt tgcccgaccg tttcgtagtc gatatcaata ccgacgacgg
     9001 catcggcccc cagcgcccgc gcctgggagc ctaattcctc aaaggcgatc tcccgtgctt
     9061 tacgcagttc tttttcatac gcaccggaac gtccgccaac gatatcgcgg ataccggcaa
     9121 agaaatcacg gaaaatattg gcacctaaaa tcgcttcgcc ggtcaccaca ccgcaatatt
     9181 caacgatggt ctggccttcc agagttgggg ttgtagaaaa ttgcatgatc gatctccttg
     9241 tttgctaagc cttcgatctc aaaagcatta tcagactgat acgctattat tgaaaggata
     9301 tcattattat cgtagaacgc tttcagagcg atcgcttata aggaaatcat tatgcgctac
     9361 agcaaattga caatgcttat cccctgcgca ctgctcctca gtgcctgcac cacagtcact
     9421 ccagcttata aagataacgg cacacgcagt ggtccttgcg ttgaaggagg cccggataac
     9481 gtggcccagc aattttatga ctaccgcatt ttgcaccgca gcaacgacat caccgccttg
     9541 cgcccttatc tgagcgacaa actggcgaca ctgcttagcg atgcctcccg ggataataac
     9601 catcgggaac tactgaccaa cgatccattc tccagtcgaa ccaccttgcc agatagcgcc
     9661 cacgttgcca gtgcatcgac tatccctaat cgtgatgccc gtaacattcc attgcgcgtg
     9721 gatctcaaac agggtgacca gggttggcag gatgaagtgt taatgattca ggaaggccag
     9781 tgttgggtca tcgacgatgt gcgttactta ggcggtagcg ttcatgccac ggcgggtacg
     9841 ctccgtcagt ccatcgagaa tcgctaacgc cttgatcaac caataaaccc cgtaaattgt
     9901 ccggcatttc gtgaggaatg ccgacattta tgctcgccga ccaccgcccc cgttattttg
     9961 tgctatgttt attgaataat gcgctttgct tttaactttt aaagcagaaa tattgcataa
    10021 ttattctgtc aaaggtacta tcttcgacct caatagctat cagactgcca gtatacgagt
    10081 gtcaatgagt attcaattaa acggcattaa ttgcttctac ggcgcgcatc aggcgctgtt
    10141 cgatatcacg ctggattgcc cacagggcga aacgctggtg ttacttggcc ccagcggcgc
    10201 gggtaaaagc tcgctgctgc gtgtactcaa tctgcttgag atgccgcgct ccggtacgct
    10261 caacattgca ggcaaccatt tcgatttcac caaaacaccc tctgacaaag cgattcgcga
    10321 tttgcgtcgt aacgttggca tggtgtttca gcaatacaac ctgtggccgc atctgaccgt
    10381 gcagcaaaac ctgattgaag cgccctgccg tgtactgggg ttgagtaaag atcaggcgct
    10441 ggcccgtgca gaaaaactgc tggaacgtct gcgtctcaaa ccttatagcg atcgttaccc
    10501 gctgcatctt tctggtggtc agcagcagcg tgttgctatt gcccgtgcgt tgatgatgga
    10561 accgcaggta ctgctgttcg atgaaccgac cgccgcactg gacccggaaa ttacggcaca
    10621 aatcgtcagc atcattcgtg agctggcaga aacgaatatt acccaggtga tcgtcaccca
    10681 cgaagttgaa gtggcgcgta aaaccgccag ccgagtggtg tatatggaaa atggtcatat
    10741 cgtagaacaa ggcgacgcga gctgctttac cgagccgcaa accgaagcat ttaaaaacta
    10801 tctctctcac taagattcgg gataacaaca atgaaaaaag ttctgattgc cgcgttaatt
    10861 gcaggtttta gtctttccgc cacagctgcc gaaaccattc gttttgctac cgaagcctcc
    10921 tatcctccgt ttgaatcgat tgatgcaaac aaccagatcg ttggttttga cgtcgacctg
    10981 gcacaagcgc tgtgtaaaga gattgatgca acctgcactt tctctaatca ggcgtttgac
    11041 agcctgatcc caagcctgaa attccgtcgc gtagaagccg tgatggcggg catggatatc
    11101 actccggagc gtgaaaagca ggtgctgttt accaccccgt actatgacaa ctctgccctg
    11161 tttgtgggtc agcaaggcaa atacaccagt gttgatcagc tgaaaggcaa aaaagtcggc
    11221 gtacagaacg ggacgacaca ccagaaattc attatggata agcacccgga aatcactacc
    11281 gttccgtatg acagctacca gaacgcaaaa ctggatctgc aaaacgggcg tatcgacggc
    11341 gtcttcggtg acaccgcagt ggtcactgag tggctgaaag ataacccgaa actggcggcg
    11401 gtgggcgaca aagtgaccga taaagattac ttcggcactg gcctcggcat cgcggtacgt
    11461 cagggcaaca ctgagctgca gcagaaactc aacactgcgc tggaaaaagt gaagaaagat
    11521 ggcacttacg aaaccatcta caacaaatgg ttccagaagt aattcctgat gaatgaattt
    11581 tttcctttag caagcgccgc cgggatgacc gtcggccttg ccgtttgtgc attgattgtc
    11641 gggctggcgc tggcgatgtt ctttgcggta tgggagtcgg caaaatggcg tcctgtcgcg
    11701 tgggcaggtt cagcgctggt aaccattctg cgtggcctgc cagaaattct ggtggtgctg
    11761 tttatctatt ttggctcctc gcagctgctg ctgacgcttt cggatggctt cactatcaat
    11821 cttgggttcg tgcagatccc agtgcagatg gacattgaga acttcgacgt tagcccgttc
    11881 ctttgtggtg tcatcgctct gtcactgctg tatgccgcct atgcctcgca aacgcttcgg
    11941 ggcgcgttga aagcggtgcc ggtgggtcag tgggagtctg gtcaggcgct ggggctgtcg
    12001 aaatcggcta tctttttccg tctggtgatg ccgcagatgt ggcgtcatgc gctgcctggc
    12061 ctcggtaacc agtggctggt gctgctgaaa gataccgcgc tggtcagttt gattagtgtg
    12121 aatgatttaa tgctgcaaac aaaaagcatc gctactcgta cccaggaacc atttacctgg
    12181 tacattgtgg cggcggcgat ttacctggtg atcaccctgc tcagtcagta cattctcaaa
    12241 cgcattgacc tgcgcgcgac acgttttgag cggaggccca gctaatgttt gagtatttac
    12301 ccgaactgat gaaagggcta cacaccagcc tgacgctaac cgttgcctcg ctgattgtgg
    12361 cactgattct ggcattgatt tttaccatca tcctgacgct gaaaacgccg gtgctggtgt
    12421 ggctggtgcg gggttatatc acgctgttta ccggtacgcc gctgctggtg cagatcttcc
    12481 tgatttatta cgggccgggc cagtttccga ctttgcagga gtatccggca ctgtggcatt
    12541 tgttgtcaga accgtggtta tgtgcgctga ttgcgttatc gctgaacagt gcggcgtata
    12601 ccacgcagct gttttacggt gcaattcgtg cgatcccaga aggtcagtgg cagtcctgta
    12661 gcgccctggg aatgagcaaa aaagatacgc tggcgatcct gctgccgtat gcctttaaac
    12721 gctcgctctc ttcttattcc aacgaagtgg tgctggtgtt caaaagtacc tctctggctt
    12781 acaccattac gctgatggaa gtgatgggat acagccagtt gttgtacgga cgcacctacg
    12841 atgtaatggt gttcggtgcg gcagggatta tttacctggt cgtcaacggc ctgctgacgc
    12901 tgatgatgcg tctgatcgag cgcaaagcac tggcattcga acggcggaat taatgtagct
    12961 gcatgctgat gccggatgcg gcggggacgc cttatccggc ctacacagtg cataaattct
    13021 tataatgaag acggacaact cactaagtta tccgtctttt tttatttcat ttaaattatt
    13081 taatcatatt tattgcatat aaattcactt gatggcattg ttatcccatg ccgcagacac
    13141 ggccaaaaat cataagattg acagacggga gttccatcat gaaaaagtta gttcttgccg
    13201 ctttacttgc ttcctttact ttcggtgctt ctgccgcaga gaaaatcaat tttggcgttt
    13261 cagccaccta tccccccttt gaatctatag gtgctaataa tgagattgtc ggctttgata
    13321 tcgatctggc aaaagccttg tgcaaacaaa tgcaggcaga atgtactttt actaatcacg
    13381 cgttcgacag cctgatcccg tcactgaaat tcagaaaata tgacgccgta atctccggta
    13441 tggatatcac gcccgagcgt agcaagcagg tatcgtttac cacgccctac tatgaaaact
    13501 cagccgtcgt gattgccaaa aaagatacct acaaaacgtt tgccgatctg aaaggcaaac
    13561 gtattgggat ggaaaacggc accacgcacc agaaatatat tcaggatcag caccccgaag
    13621 tgaaaactgt ctcctatgac agttatcaga atgcctttat cgatctgaaa aatggtcgta
    13681 ttgatggggt atttggtgac acagcggtgg taaacgaatg gctgaaaacc aatccgcaac
    13741 tgggcgttgc tactgaaaaa gtgaccgatc cgcaatactt cggcaccggt ctgggcatcg
    13801 ctgtacgtcc ggataacaaa gctctgctgg aaaaactgaa taacgcgctg gcagcaatta
    13861 aagctgacgg tacatatcag aaaatcaatg accagtggtt cccgcagtaa gattcagaaa
    13921 gccgcaaccg cggcttgaga ctgatgacaa aggtaaaatt gcctgatgcg ctacgcttat
    13981 caggcctaca tggcccatgc aatgtattga atttgcacgc ttttgtaggc cggataaggc
    14041 gtttacgccg catccggcat aaacaacgcg cactttgtca gcaatctgaa gccgcacctg
    14101 cggctttttt attgcttcac cagcagcgtc agcacttcat agtgcgcggt gtgcgggaac
    14161 atatcaaaaa gctgtacccg ttcgatgcgg taaccaggca gttctcggac atctttcgcc
    14221 atggtttggg cgttacagct ggagtagatg ataaaacgcg gtgccatcgt tgagagataa
    14281 tcacacagcg gtttaccaat gccgcggcgc ggcgggttaa ccagcaccag ctccggcacc
    14341 tccccctgag cagtggcaaa ctgagtggag tccagcgcct gaaattgcaa acgcgttaag
    14401 cccagttcag cggctgactg ctttgcacag gcaatggcct ctggcgcaat ttcgatcccg
    14461 gttaactgca tgtcaggcgt cgcgcagtgt aaaccaaagc cccccacgcc acagaacaga
    14521 tcccacatat gtttaaccgg cagctgtcgt acccagtcgc gcgcggtagc gtacagctgg
    14581 ctggcgaccg ccggattagt ctggaagaaa ctttgcggac ggacccacag cggcacatcg
    14641 ttaaaacgct ccgccagtgc ctgttgttcg gtcaggtaga tctccgtctc cccttccata
    14701 atcgccatat gtaccggctg aatattgacg gtaataactt ttagctgcgg tagttgttcc
    14761 tgtaaccacg gtagcgcctt acgcagttgc gccagtttgg tttcggaacg caacacaaaa
    14821 cgcagcatca tgccgccatc gctctggctt tcagtcagca aaatgtattt cagttcgcca
    14881 cgcttacgcg ccacgttgta gggcgttaac cccgctcggg cgataaacgg ttttagcgcc
    14941 gcaaaaacgg gcgcaaatga ggcaggataa agcgggcagt cacaaaggtc ttccggtgtg
    15001 ccgtctcgat gcagcatacc gagcagtggt ttttcaacgc taccactcac caccattttg
    15061 gctttattac gaaacccttg ttccaggcct gacaccggcg cgcaccattc ctcaaccgga
    15121 aagtcggcga gcagattttt aagatcggcg gttttagcgg agagttgctc tggaatcggc
    15181 tgcgttatcc actgacagga acgacagcga cccgcgtcgt aaagtgcgca ctgcatacat
    15241 tgaccttcac atcatcaggg gcgacgatta tacacattat tgcaacagga agaaccgccg
    15301 actggtcgag ggaacgaaca gcagcatcag gatgagcata tccggcagct tctgcagcat
    15361 caggctatgg aagatttcac gttttgattc accgggaatg ctgaacagct ccggataacc
    15421 ataccccagc gaagccgccc acaagtaact tgcggcggtg atttgtgtca gcagatatag
    15481 ccagcgcgcc cagcgacgcc ctttcaccag tgaaaacgca caccagatct cgataaaaac
    15541 cagcaccaga ctgcttaaaa agaccagcgt taagctccag gtttgtacgc tgcgatgaat
    15601 gaactcgcca ataccgcgca cgcccagcgt attgaaaatc atcagcacgt cgaggccacg
    15661 gatcataata atggcgagcg ccgctacctg caccagcgca ggcacattca ggcgagcatg
    15721 agatgaagat gttttcttaa aaaatcccaa cgtttcgtct tccatgaaaa caatgccgcg
    15781 gcatgcgcgg cattatgtag ccaggttggc aaattttagt gtcttcagcc acgtcttgca
    15841 cgctggatat cgcgtatccg ctgtttttct gcgcgagcca tcagatacca ggcgataaat
    15901 ccgacaattc cgaccgcacc gagaattaac gttgcaaggg cgttgatttc cggattcacc
    15961 cccatccgca cgctggaaaa gaccagcatc ggtaacgtgg tggctcccgg cccagaaaca
    16021 aagctggcga tcaccagatc atcaagcgac aaagtaaaag ccagtaacca gccagaaatg
    16081 atcgcgggca tgatcatcgg tagcgtaatg acgaaaaata ctttcagcgg cgtcgcaccg
    16141 agatccatcg ctgcttcttc tatcgagcta tccagttccc gcagacgcga cgaaataacg
    16201 actgccacat aagccgtaca gaacgtgaca tgcgccagcc agatggtaag catcccgcgg
    16261 tccgcaggcc agccaatggc atgagcaagc gcgacgaata acaacaacag cgacaagccc
    16321 gtgatgacat ctggcatcac cagcggcgcg gtgatcataa aggcaaagcc atttgatccg
    16381 cgaaacctgc caaagcgcac cagcaccacc gccgcaatag tcccgaggat cgccgccgcc
    16441 gttgccgcac aggcagcaat tgtcaggctt aaaccaaccg cactcatcat cgcgtcatcg
    16501 cgcaataact caccatacca gcgcgttgac cagccggccc acaccgtcac cagtttcgag
    16561 ctgttgaatg aatagatcac cagcatcagc attggcgcgt agagaaaggt gaagcccaac
    16621 agcaaaatca caatccgcca gggcgaacga actaccggta aattattcat ccgtgttctc
    16681 ccacgctttt ttgctggtgt ttgtgaaacc acattatcgg cacaattagc agcagcaaca
    16741 tgatgatcgc taccgccgag gccaccggcc agtcgcggtt gttaaagaac tcctgccaca
    16801 gcacgcgccc gatcatgatg ctgtccgggc caccgagcag ttccgggatc acaaactcgc
    16861 ccacagccgg gataaacacc agcatcgatc cggcaataat cccaccttta gtcagcggca
    16921 cgatcacagt aaagaacgtt ttcagcggtc gtgcaccgag atccagcgct gcttccacca
    16981 gcgaataatc aatacgaatc aacgcggtat aaatcggcag taccataaac ggcacgtaag
    17041 cgtaaacaat gccgatataa acggccagat tggtatgcag aatggtcagc ggttgatcga
    17101 taacccccag ccacagcaga aaattattca gcacaccgtt gttttttaat attcccatcc
    17161 aggcataaac gcggatcaga aacgaggtcc acgacggcag gatcaccagt agtaataaaa
    17221 tattacgggt cgaaggctta ctgtgcgcca ccgcccacgc cagcggatag ccgatcagta
    17281 aacagcaaat agtcgaaatc gccgccacct gtaacgactg gagataagca tcaaaataga
    17341 gcggatcgtc ggtcagttgc agaaaattac cgagattaag agtgatggaa agttgcccgt
    17401 cagcccactc catcagctcg gtataaggtg gaatagcgcg cgccatctct cccaggctta
    17461 ttttaaagac aatcagaaat ggcagcagaa acagcaagat caaccagata tatggcaacg
    17521 caatgaccag tttgcgccca tgcttcattt gcagctgcga caaccacagc ttaaatccgc
    17581 ccggcggttt cgactgggca gcaggttcaa gtgtactcat ctttgctcct taaaccgtca
    17641 gcaccacaca gctgtccact tcccagcaca aacgcacttc gtcgccccag gtcggtaacc
    17701 ctttacgatg acgatgggcg ttttgtagct gggcgctgat catctgccca cttttcagtc
    17761 gaacgtgata caccgaaaga tcgccgagat aggcaatgtg tatcacctcc cccaccgcga
    17821 agttacaacc attggcgggc ggctcttcgc aaagcatgat tttttccggg cgcagcgcta
    17881 cgtgtaccgg cacgttatcg accactgaag catccgcatc gactttcagt ggatgcacca
    17941 gccccggcga atcaagcacc aagccatctt cctgacgctc tttgagtaca ccttcaaaga
    18001 cattaaccga accgataaat tcagcgctgt agcgggtagt tggatgctcg tagatctctt
    18061 ccggctcgcc aatctggaca aatttcccgc gattcataat ggcgatacgc cccgccatgg
    18121 tcatcgcctc ttcctgatcg tgggtgacca tcacacaagt cacaccgacg cgctccagaa
    18181 tatccaccac ttcaagctgc atccggtcac gcagcttttt atccagcgcg cccatcggct
    18241 catcgagcag taatagtttc gggcgtttgg caaggcttcg ggccagggcc acacgctgtc
    18301 gctgaccacc ggaaagctga tgcggtttgc gtttggcgaa ctcctgcatg tgcaccaggc
    18361 cgagcatctc attgacccga ctggcaattt ccgctttcgg tagtttgtcc tgtttcaggc
    18421 caaaagcgat gttctgttcc acggtcatat gcggaaacag cgcgtaagac tgaaacatca
    18481 tattgatggg gcgcaggtaa ggcggaacct gtgacaaatc gacgccatca agcattatct
    18541 gtccggcaga aggttgttcg aaacctgcca gcatacgcag cagcgtggac ttgccacagc
    18601 cggatgcgcc cagcagcgcg aagatttcac ctttgtagat agtcaggctg acatcatcca
    18661 ccgcatgttg accatcgtag gatttggtga ggttgcggat ttctaatagc ggcgtcagcg
    18721 ccttacgggt tttcgcctgc gggcgaggga tagcgtcatt cacggctgct ctccggcatt
    18781 gaatgtgtgc aagcgcacca tgatggtgcg aattgccggc gtgtgtggtg cacccgtccg
    18841 gcatctacga ctgcggatta ttttccgctc ttcactttgg tccacgcgcg ggtgcgcaca
    18901 cggtctattt tcgggtcctg cactttcaga gtgaacagct tcgcacggac atccgcaggc
    18961 ggataaatac ccgggttatc acgaacttcc gcactcacca gcggcgtggc tgctttattg
    19021 gcgttggcat agaacacatg gtcggaaata tgcgctacta catccgggcg cagcaggtaa
    19081 ttcaggaact gataggcttc gtctttgttt ttggcatccg caggcatggc gaatacatca
    19141 aagaacgcca tcgccccttc ttttggaatc gagaacgaga cattcactcc attcttcgct
    19201 tccttcgcgc ggtttgacgc ctgccagaca tcacctgccc agccgatagc gacgcaaata
    19261 tcgccgtttg ccaggtcgtt aatgtattga gatgaatgga aataacgaat gttcgggcgc
    19321 agctttaaca gcagatctgt tgccggtccg gtgtaatcat ccgctttagt gctgttggga
    19381 tctttgccga gataattcaa cacggtagca aaaacttctt ctggcgcatc caggaaagag
    19441 acaccgcagc ttttcagttt ttccagattt tcaggtttga ggatcaagtc ccagctatcg
    19501 acgggcgcgt tttcgcccag caccgcttta actttatcaa cgttatagcc aatcccggtc
    19561 gtcgcccaca tatagggcat agcaaattta ttgtcgggat cgtgtttggc gaccagcttc
    19621 agcagttccg ggtcgagatt cttccactcc ggcaatttgc ttttgtccag cggctggaaa
    19681 actcccgcag tcaactggcg ctccagaaag ctggcagatg gaaccaccag atcaaagccg
    19741 gtactcccgg ccattaattt gccttccagt acttcgttag agtcgaaaac atcgtagacg
    19801 actttaatac cggtttcttt ttcaaaattg gccaccgtgt ccggggcgat ataatcagac
    19861 cagttataaa tgtggagtgt tttttgttca gccgcgagcg tgccgacaga gacggccatc
    19921 agagcacccg caaccagacc cgatagccat tttttattta aggcggtcat atctctttcc
    19981 ttctgaaagt tcgttaacaa acgaacaaaa gttgtcacac tgaaagatat gcaataaacg
    20041 tgcatatttt ttatcggcgc ggtgtaagaa aagagaactt tctcccggcc agcactgctc
    20101 gctatataaa agcatcgcaa gaataacttt agccagggtt tgagactata gctcggatta
    20161 aaaaacggta tttaacaaat ttttatgcat atttgctcta tgtgataaag ccaaatcgac
    20221 acaagagaag tgaaataatc tttaagaaaa ggttaaaaac agcagataat tttgcagcgc
    20281 gcagccactg caccagcgac tgcgtgctcg aaaggagtta tcagtggagg aaatagtttt
    20341 cggtaacgtt gttttgccct tcatcatcag tcggtaacag cagttgatgg gcattagctt
    20401 caagaatgac catcgaaatc tgctcttcgc tctgacgcac gaaccatgca aactgctcat
    20461 aggttacgcc ctgcataacg gataaagact ggcaaaccac cagctttggc agattatcat
    20521 cctgcatatc aagaaatgct ttcacggtca gcgaactggc attgatggca gataaatccg
    20581 ccgccagcgg caatacggct gaaggtcgga cttccgccat ggcagaaaac aggatcgtgt
    20641 tatcgatcag atcgattttg gcatcaaaga caccgtcgaa attctgcata tggggcagat
    20701 gcagagcctg acagttatca cattcaaaaa aactcatccc caggtcatcg agccattgac
    20761 gcagcgtatc cagaccagga acgaccagcg atgtcataat tttgtacaac ctctttcgat
    20821 aaaaagaccg gcacagctta cgcaaaaagc gcaggcaaaa ccatgatcag taatgtgatt
    20881 gcgactaacc acccgttttc aggcaatatt ctgtcgtagc gtggcgttcg atccagcgga
    20941 tcattttacc cgcgatgtcg ataccggtgg ttttttctat tccttccagc cccggcgacg
    21001 cattcacctc catcaccaac ggcccgcgat tagcacgcag aatatccaca ccagcaacgt
    21061 ccagcgccat cgttcgcgcg gcttttatcg cgatttcacg ctcctgtggt gtgatactgg
    21121 cgacgcttgc cgcgccgcca cgatgcaaat tggaacgaaa atcgccctct ttcgcccgcc
    21181 gttcaatcgc agcgaccact tcatcgccaa caaccagaca gcggatatcg cacccttgcg
    21241 cctctttgat atattcctgc accagaatat gcgcgttcag accgcggaaa gcgtcaatca
    21301 cgctttccgc cgcctgacgc gtctccgcca gcacgacgcc aattccctgc gtgccttcaa
    21361 ccaacttgac caccagcggc gcaccaccga ccatgccgat taaatcgctg gtatcatccg
    21421 gcgaatgcgc aatgcccgtg acaggcaggt cgatgccctg acgcgccagc agttgcatgg
    21481 aacgcaattt gtcacgcgcc cgggcaatgg cgaccgactc attgagcgga tagctcccca
    21541 gcatctcgaa ctggcgcagt gccgccgtcc cataaaaggt aatggcggtg ccaatacgcg
    21601 ggatcactgc gtcaaaatgg ggtaacttgc ggcctttgta gtgaatagaa gacgccgcag
    21661 gatttatgtt catgtagcaa gaaagcggat caagaatttc aaccaggtga ccgcgctgta
    21721 tagcggcttc acgcagccgc ttacacgaat agagcgttcc atcccgggac aatatggcaa
    21781 ttttcaccct gcacctctct gtcagacctg gtcaaaagcc cgcgtatcat acatcgacgt
    21841 ggcggtttta gcgcgtcgcc caaccctgtt tgtgcaaata atccagaata aatgggcggc
    21901 tttctttaat gattgttcgg cggatatgat cgctccaggt atcccggcga ttattgctgc
    21961 cacgggtgag gtaatattca gccagttgtt cgtcatactg cgccagtgcg tctttatcca
    22021 gcggttgata gctgttttca tgcaccaaaa tggaggacgg taaacgcggc ttaagatccg
    22081 gattatccgc aggccagcca aggcacagcc caaacagcgg cagaacatgc tgcggtaatt
    22141 tcagcagttt cgtcaccgct tcaatattat tgcgcaggcc gccgatatat acgccgccca
    22201 atcccagcga ttccgctgcg attaatgcat tctgcgccat cattgccgta tcaacgacac
    22261 cgagcaacag ttgttccgcc aggccgagct gagcatccgg acagatctgt aaatggcggt
    22321 taaagtcggc acagaacacc cagaactccg ccgcttgcgc tacgtgtttt tgcccgccgg
    22381 tcagcgtcac cagttcttca cgtaacgctt tgtcggtaat gcgaataatg ctactgcact
    22441 gcaaaaaact ggaactggac gtcgcacggg cgctgttaat aatcgcctca cgctgcgctt
    22501 cggaaatggg ttcatcagtg aaatggcgaa tggagcgatg gccacaaata agttcaatgg
    22561 ttggcgtcat tatctttttc tctttctgaa cgtgaatatt gcggtggacg gttcatcagc
    22621 tgtggggcaa gacgttttgc cacctgaaga ataaccacca ccgcagcggg aagcatgagc
    22681 aaaacaccga gaaaaatcat cagaatctgc acttctggcc gagaaaatgg ctcaggcagc
    22741 gacagggagt cacttaccga cagcagcgcc accgccagta gcatcattcc gataaattcc
    22801 agtatcaaca cgcctttagg caatttaccg atcgcgcgca tacgcttccc tctgcaaagt
    22861 gagccttcag tctaaaactt ttcactgtat tgtgtttaac agttatagct tttagcaatt
    22921 aatgcaacaa gttaaaccta ctttcagcga atacatttta gcgtgatcat tacaggcata
    22981 aatctatgag gagagaaata atgcaaaccg ttatttttgg tcgtccgggt tgcccttact
    23041 gtgtgcgtgc aaaagatctg gctgagaaat tgagcaatga acacgatgat tttcagtatc
    23101 actatgtaga tattcgtgcg gaagggatca ctaaagaaga tctacaacaa aaggcaggta
    23161 aacccgtaga aaccgtgccg cagatttttg tcgatcagca acatatcggc ggctataccg
    23221 attttgctgc atgggtgaaa gaaaatctgg acgcctgatt gtctgacaag ccctcgcgtt
    23281 gagggcttta ctgatttttt ctgtgctgtg gtttaaacaa actactgata aataagaaac
    23341 acagtgcccc cagcgcacac cagaacaccg cacttaataa ccatgccagc tcttgccaga
    23401 atgagcgcgt cggtgaaaaa aacagccgca taatgagcat cgaacagggt gccgccagca
    23461 ttgcgccaaa cagaggtttc aggacttctc tacgctgtga aaagaagctg gcgactgctc
    23521 caggaagaat gaaaaatagc aagccgattt caggatgccc ggcagcccga aaagcgcctt
    23581 tcatgtgcgt cgccagaaaa aggcacacca caatgaagag gacaaaacag cagattgccc
    23641 ccgcccaacg ttgtttatgt ttcactcgtt cctcctgaca ctgcgtctat cgaacacatt
    23701 tttcgccagt gtggcgttca gtaagataaa gccgcttcgc attccatgct aatataggcc
    23761 aacgcaattc atatagccgt tgatacctaa tgtgattaca ctagtaaaat atattgttac
    23821 tttactatcg tttaggtgcg ctgaatgaat ctgcgccctg aattctggta aaaaacatta
    23881 tcgtaaatta ccatttcttt caacagctta ctagtaaaca agaagttagc ctccgtgaat
    23941 ataaacgtcg ccgaattgtt aaataggaat tacattctgt tattatttgt ggtcctcgcg
    24001 cttgggctat gtctcggaaa gttacgactt ggttcgatcc aactgggtaa ttccattggc
    24061 gttttagtcg tatcgctgtt attaggccaa caacatttca gcattaacac cgatgcgctt
    24121 aatcttggct ttatgctgtt tattttctgt gttggggttg aagccggacc gaactttttt
    24181 tccatttttt ttcgcgatgg gaaaaattac ctaatgttag cactggtgat ggttggcagt
    24241 gcgctggtga tcgccttagg gttaggtaag ctgtttggct gggatattgg cctgacggcc
    24301 ggtatgttag caggctctat gacgtcgaca ccggttctgg tcggtgctgg cgatacactg
    24361 cgtcattccg gcatggaaag caggcagctc tcactggcac tggataatct gagcctcggg
    24421 tatgccttaa cctatttaat cggtctggtg agtttgattg ttggtgcgcg ttacttgccg
    24481 aaattgcagc atcaggactt acagaccagc gcccagcaaa tcgcccgcga acgtggcctg
    24541 gacactgatg ccaaccgtaa ggtttattta ccggtgatcc gcgcctatcg cgtcggcccg
    24601 gagctggtgg cctggaccga cggcaaaaat ctgcgtgaac tgggtattta tcgacaaacc
    24661 ggctgctaca ttgaacgtat tcgacgtaac gggattctgg caaatccaga cggtgatgcc
    24721 gtgctacaaa tgggcgatga aatagcgttg gtaggctatc ccgacgccca tgcccgactc
    24781 gatcccagct tccgtaacgg taaagaagtt ttcgatcgtg accttctcga catgcgtatc
    24841 gtcactgaag aagtggtcgt taaaaaccat aacgctgtag gtaaacgtct cgcacaactg
    24901 aagttgaccg atcacggttg cttccttaac cgcgtcattc gtagccagat tgagatgccg
    24961 atagatgaca acgtcgtgct taacaaaggt gacgttttac aagtcagcgg tgatgcccgt
    25021 cgcgtaaaaa ccatcgccga tcgcatcggc tttatctcga ttcacagcca ggtcactgac
    25081 ctgctggcat tttgcgcctt ctttgttatt gggctgatga tcgggatgat caccttccag
    25141 ttcagcacat tcagtttcgg catggggaac gctgccgggt tgttattcgc cggaattatg
    25201 ctgggcttta tgcgtgctaa ccacccgacc ttcggttaca ttccgcaggg tgcattaagc
    25261 atggtgaaag agttcggctt gatggtgttt atggcaggcg ttggtctgag cgccggtagc
    25321 ggtattaata acggcttggg cgcgattggc ggtcagatgt tgattgccgg attgattgtc
    25381 agtctggtgc ccgtggttat ctgtttcttg ttcggtgctt atgtattgcg aatgaaccgc
    25441 gcgctgttgt tcggcgcaat gatgggcgca cgtacctgcg cgccggcaat ggagatcatc
    25501 agtgatacag ctcgcagtaa catcccggcg ctgggctatg cgggcaccta tgcaatcgcc
    25561 aacgtcctgc tgacgctggc agggacaatc atcgtcatgg tatggccagg attaggataa
    25621 aactgaagtt gccctgaaaa tgaaattttt ttgcacaacc gcagaacttt tccgcagggc
    25681 atcagtctta attagtgcca ctgcttttct ttgatgtccc cattttgtgg agcccatcaa
    25741 ccccgccatt tcggttcaag gttgatgggt tttttgttat ctaaaattta tctactaccc
    25801 tgcaaccctc tcaaccatcc tcaaaatctc ctcgcgcgat agcggcttac ggtcggtgac
    25861 aaaatgcagc gtcatcccct caataaacgc atcaagcgcg cgggcggttc cgggttcaaa
    25921 ccattgttcg agcgtttgct gactgcgctg catccagttt tgcattaccg tttttaatag
    25981 cggttttcgg ctagccagcg cgtagagctg gtacatcagc tccatattat ccggcgttgc
    26041 aacctgtgag ctgtagatca tatcggtgat agcctggcat gcgccttgag catcactaac
    26101 atcgctaaaa aatgcctgat attgccggga cataatctca gtaaaactgc tgaacgcctc
    26161 cagcaacaac tcatcaattc ctgaaaaata gtaggtcatc gatcccaacg gtaccccggc
    26221 aagggtagca attttgcggt gcgtaacagc atgtattccg taaagtttca ccgcctccag
    26281 cgtggcctgg ataatttttt ctcgccgttg cggatcgtta gcgcgacgca tgtcatttcc
    26341 tctgtctgca attgtgtaca aatgtacaca accttgctaa tgttgtcttc cctcttctta
    26401 ttttgacgtg atctatgacc gtaaattctt cacgtaatgc attgaaacgc cgaacctggg
    26461 cgctgtttat gttcttcttt ttgccaggcc tgttaatggc gtcctgggca acccgtacgc
    26521 ctgctatccg cgatattctc tctgtctcga tcgctgaaat gggcggtgtt ctctttggtc
    26581 tgtcgatcgg ttcgatgagc ggtattctct gctcggcgtg gttagtgaaa cgctttggga
    26641 cacgtaatgt catcctggtc acgatgtcct gcgcattgat cgggatgatg atattaagtc
    26701 tggcactctg gctgacatcg cccctgctct ttgccgttgg tctcggcgtc tttggggcaa
    26761 gttttggttc tgcggaagtg gcgataaacg ttgaaggtgc cgccgttgag cgagaaatga
    26821 ataaaacggt tttgccgatg atgcacggtt tttatagcct gggcacgctg gcaggcgctg
    26881 gtgtcgggat ggcactgacg gcctttggcg ttccggcaac ggtgcacatt ttattggcgg
    26941 cgctggtagg tatcgcgcct atttatatcg ccattcaggc aatccctgac ggtacgggca
    27001 aaaatgctgc cgatggcacc cagcatggcg aaaaaggcgt acctttttat cgcgatatcc
    27061 agttgctgct gattggtgtt gtggtgctgg cgatggcctt tgccgaaggt tctgccaacg
    27121 actggttacc cttattaatg gttgacggtc acggttttag ccccacttcc ggctcgctga
    27181 tttatgccgg ttttaccctg gggatgaccg ttggacgctt cactggcggt tggttcatcg
    27241 accgttacag tcgcgttgcc gtggttcggg ccagtgcgct aatgggggcg ttgggtattg
    27301 ggctgattat ttttgtcgat agcgcctggg tcgctggggt gtctgttgta ctctggggaa
    27361 tgggtgcctc gctgggcttc ccgctgacca tttctgccgc cagcgatacc ggccccgatg
    27421 caccaacccg cgtcagtgtg gtagcaacga ccggttatct ggctttcctc gtcgggccgc
    27481 cgctgctggg ctatctcggc gaacattatg gattacgtag tgcaatgctg gttgtactgg
    27541 cgctggttat tctcgcggct attgtcgcga aagtcgtcgc caaacccgat accaaaacgc
    27601 agacggcgat ggagaatagt tgatgagcat taaattaatt gcggtagaca tggatggtac
    27661 tttcttaagc gatcaaaaaa cctataaccg tgagcggttt atggctcagt atcagcaaat
    27721 gaaagcacaa ggcattcgct ttgtggtcgc cagcgggaat caatattatc agttgatctc
    27781 tttcttccct gaaattgcta atgaaattgc ctttgtggct gaaaacggcg gctgggtagt
    27841 gagtgaaggc aaagatgttt ttaatggcga gctgtcgaag gatgccttta ctactgtcgt
    27901 ggaacatttg ctgacgcgcc cggaagtgga aattattgcc tgcggaaaaa atagcgccta
    27961 tacgctcaaa aaatatgacg atgccatgaa aacggtggcg gaaatgtatt atcaccgtct
    28021 ggaatacgtc gataactttg acaacttaga agatattttc tttaagtttg gcctgaatct
    28081 ttccgatgaa ctgattccac aagtacaaaa agcattacat gaggccatcg gcgatattat
    28141 ggtgccagtc cacaccggca acggcagcat cgatctgatt atccccggcg tacataaagc
    28201 caatggcctt cgccaactgc agaaattatg gggaatagac gacagcgaag tggtggtctt
    28261 tggcgatggc ggtaacgata ttgagatgct gcgtcaggca ggctttagtt ttgcaatgga
    28321 aaatgccggc agcgcggtcg tcgcagcggc aaaataccgg gcaggctcca ataaccgtga
    28381 aggcgtactg gatgtgatcg ataaagtgct taaacacgaa gcgccatttg accaataaaa
    28441 acggcagcac gcccggcaac acaatacctg aatgaaggct gcataattcg cggaaattaa
    28501 cccgctgcat tatttcaggc gaaatgatta tgaaaaattg tctactgttg ggcgcgcttt
    28561 taatgggctt tactggtgtg gcgatggcgc aaagtgtcac cgtcgatgtg ccgagtggat
    28621 acaaagtggt tgtagttcct gattcagtca gcgttccaca agcggtcagc gtcgcgactg
    28681 tgccgcaaac cgtctatgtg gctcctgcgc cagctccggc ttaccgcccg catccttatg
    28741 ctcgccatct tgccagcgta ggtgaaggga tggttatcga acaccagatt gacgaccatc
    28801 accattaatt cgagaatgcc tgatcgcaca aagcagtcag gcattttttt acccttcgtg
    28861 agaatttccc atctgtttat cttttaaaaa gataaccatc agcgacagcc acaaaattcc
    28921 gttgacaagg ttgaagagat taaactgtcc gttgcccccg ttcagccagg catgtttgct
    28981 gatttcaata ccaacggtaa agatcagcat ttgcagcatt cccatcgccg cagaaaccgt
    29041 acctttactc atatcgctgg caaacagggt taatcgcacc agtcccgcat tcgccagacc
    29101 aataccgaaa gcataaatac ttaacccggc agtcatccat aaatacgcgt gcgatgagat
    29161 aaccgttgcc gcagcagcga ccaacagacc aatcataatc ggccagccgc ccataattat
    29221 cagcgaacgt acggtgcggc gcgaggtcag acgcgctaac agcaagttac ccgcaattaa
    29281 cgccccgaaa ataggcactt gcagcaagcc atattcatag ctgctcaact gctcgccggt
    29341 aatgatgata atcggcgact gggcgatcca cgccagcaat ggcaggctaa cgaatcccag
    29401 cgccagcgcc cccgccacaa agcggccgtt cttcagcacc agcttatagt cacgaccgag
    29461 ttctttcagt gacagtttct cgcctatacg cgtggcggtt tcaggcatgg ctcgttgcag
    29521 accgaaaaag gagatcgctg ccaatgcggc aaacaaaaca aacatcccct cccagggcag
    29581 cacatggatc cacgccgcgc ccaccagcgg accaagtagc ggagcaatca gcgccacgtt
    29641 cgccatcagc gcggtgatct tgatacaaac cgcctcttcg aaggattcct gaattgcggc
    29701 gtatcccaca gcgccaatga aacagaggct tatgccctgc aagaagcgca acagggtgaa
    29761 ttgttcaatg ttttgcgcca gcaatattgc cagacaggtg acgataaacc acaccactcc
    29821 cgccagcatc accggacggc gaccaatacg atccgacagc ggccccagca gccattgtaa
    29881 aaacatcccg cccgccagat acgcggtcat cgaagtagga acccaatcaa tgcccgcctg
    29941 atattgttcc accacggcca acataccggg ttgaatcata tcgttgccga tataggttga
    30001 aaattcgtaa agcaccagac agagagggaa aagtaacgcc tgacgtccaa gcctggcacc
    30061 ggaagctaat ttattttgca tgcaatttct tcgccaataa taatcgcgca gagtttaata
    30121 aaagcgcagc taacgagaaa gcgaattttg tagctgaaac cacggttaag cacattctta
    30181 cattattgcg agtatagcta cgctttcttt aagttttatt taacctatgc ccgttacaat
    30241 cacccaccgt aaacaggccg cttgagggaa ataagacgat gccgctttac ccagtttaac
    30301 ctgcacttta ttctcaacga cttgcctgta ttggctccct tttaatcact ttgcgtcggg
    30361 aagttatgct ggaaaatttg aatctctctc tattctctct tattaacgcg acgccagact
    30421 cggctccgtg gatgatctcg ttggcgattt ttattgctaa agatttgatt accgtggtgc
    30481 cgttgctggc cgtggtactt tggttgtggg ggcttacagc acaacggcaa ctggtgataa
    30541 aaatcgctat cgcgctggcg gtcagcctgt ttgtgtcctg gacgatggga catctttttc
    30601 cgcacgaccg accctttgtc gaaaatatcg gctataactt cctgcatcat gcggcggatg
    30661 actcattccc aagcgatcac ggtacggtga ttttcacctt tgcactggca tttttatgct
    30721 ggcatcgcct gtggtccggc tcacttttaa tggtgctggc cgtcgtcatt gcctggtcgc
    30781 gcgtttatct tggcgtccac tggccgctgg atatgctcgg tggattgctg gcaggtatga
    30841 ttggctgcct tagtgcccag attatctggc aagcgatggg gcataaactc tatcaacgtc
    30901 tgcaatcgtg gtatcgcgtc tgttttgcat taccgatccg caaaggctgg gtgcgtgact
    30961 gatctctgct aaaaagtgta gtattgagcg gctcgcttca ataactattc agagggatta
    31021 tggaaacacg tcgcgaagag cgtatcgggc agctgctgca agaattaaaa cgcagcgata
    31081 agttacatct taaagacgcc gccgccctgc ttggggtttc ggagatgacg attcgtcgcg
    31141 atctgaacaa ccacagtgcg cccgtcgttt tgctcggcgg ctatattgtt ctggaaccgc
    31201 gcagtgccag ccattacctg ttaagcgatc aaaaatcccg cctggtggaa gaaaaacgcc
    31261 gggcggcaaa actggctgcg acgctggtag aacccgatca gaccctcttt tttgactgtg
    31321 gcaccaccac gccgtggatt attgaagcga ttgataatga aatccctttt accgccgttt
    31381 gttattcgct aaataccttt ctggcgctga aagagaaacc ccattgccgc gcgtttcttt
    31441 gcggtggtga atttcacgcc agcaacgcca ttttcaaacc catcgatttt cagcaaacgc
    31501 tgaataattt ttgcccggat atcgcttttt attctgcggc gggcgtgcat gtcagtaaag
    31561 gcgctacctg ttttaatctt gaagagttgc cggtaaaaca ctgggccatg tcgatggcgc
    31621 aaaagcatgt gctggttgtc gaccacagta aatttggcaa ggtgcgtccg gcgcgcatgg
    31681 gtgacctgaa acgctttgat attgtggtga gcgattgttg cccggaagat gagtatgtga
    31741 agtacgcgca gacgcagcgc attaagttga tgtattaatg acgtataacc ggatgacgtt
    31801 tcgcgccatc cggttatcag aagattaaga gaaccagctg ccgaaccact gatggaattt
    31861 catcatcacg aaatcccaca cccgaccaaa gaatccgccc tcttccacat tttccatcac
    31921 gatcagcgga cgctgctcaa tggatttacc gttaagctgg aaatcaatgg tcccgacaac
    31981 ctgacctttt ttcagcggtg cggtaagctg cggttccgtt aacgtataac tcgctttcag
    32041 gtttttcagc tgcccacgcg gtatggtcac ggagcccgct tcgcctgccc cgagattcac
    32101 ttcgctctta tcaccaaacc agacgcgctg agtcacaaag gtggcatcag gtttaattgg
    32161 cgtcacagtt tcaaagaagc ggaaacccca ggtcaataat ttctcagact cattaaaacg
    32221 gatacggtcg gttttcgccc ccagcactac ggagattaaa cgcatatcgc cctgggtagc
    32281 cgaagcaacc agattatatc ccgcgcctgc cgtggttcct gtcttcatgc catcaacatt
    32341 cagattgctg ctccacagca gacggttacg gttaggctga cgaattttgt tgaaggtgaa
    32401 ctctttctct ttatgaatgg cgtactcttc cggcacatcg tggatcaatg ctttgcccag
    32461 caatgccata tcgcgcgcgg tactgaactg ccccggcgca tccagaccgt gcaccgtctg
    32521 gaaggtagtg ttggtcagac ccagtttttt ggcataacca ttcatcaaac caataaatga
    32581 ctcctggctc ccggcaacgt aatcggccag cgcaatacag gcgtcattac cggactggat
    32641 aatcacacct ttgttcaagt ctgccaccga aacctgatcg cccggtttga ggaacattac
    32701 cgatgaacca cgcagtgccg gatttcccgt cgcccaggca tctttaccga ccgtcaccat
    32761 atcggtgagt ttaatcttat cggccttaag cgcctgccca accacatagc tggtcatgat
    32821 tttagtcagg ctcgcgggat ccagtttctc atccgcgttg ccttctgcca gcactttacc
    32881 gctggcgtaa tccattaaaa tccatgcacg cgcatccacg ctcggcgctt caacggtttg
    32941 ttccgccgcg aatgccgttg gggcaaaaag gaataaaaat gcagaacccg ctgcaagacc
    33001 acgaaggaga gaggagtatt gcgtcataaa tgccacccga gtatccattc aaaaaaaata
    33061 cgttatatcg ccgtatgata agttcggtga ttaataacgc acacacaaac ttaaagaaac
    33121 cgtaagttgg taaagttttt aaagtttatg caatatcctg ccgcttcgct gcttctggtg
    33181 tcattttttt gccagctgtt gcttaaaagt taactttact tcaccatcgg agtcctgttc
    33241 agcctgtggc tgcctgtcgg aaaaggggtg agtatgatta cgctgtgggg tcggaataat
    33301 tcaactaacg taaaaaaagt attgctgacg ctcgaagaac tggaactacc ttatgagcaa
    33361 attctcgcgg gccgtgagtt tgggataaat cacgatgctg attttctggc gatgaaccct
    33421 aacgggctgg tgccgttgtt gcgtgacgac gaaagtgatc tcattctttg ggaatcaaac
    33481 gccattgtcc gctatctggc ggcacagtac gggcaaaaac gcctgtggat cgactcaccg
    33541 gcacgtcgtg cggaagcgga aaaatggatg gactgggcaa accagacgct cagtaatgct
    33601 catcgcggga tcctgatggg attagtcaga acaccaccgg aagagcgcga tcaggccgcc
    33661 attgatgcca gttgcaaaga gtgcgacgcc ctgtttgccc tgctcgatgc ggaactggca
    33721 aaagtaaaat ggttctctgg cgacgagttt ggtgtgggcg atatcgctat cgcaccgttc
    33781 atctacaatt tgtttaacgt tggcctgacc tggacaccgc gtccaaatct gcaacgctgg
    33841 tatcagcaac tcactgaacg ccccgcagtg cgcaaagtgg tgatgattcc cgttagctaa
    33901 ttgcgtgggc taactttaag taattcccca ctggactcgt cggtgagaac gtataaataa
    33961 ccgtcgggtc cagtgcgaac atcacgaatt cgctgccctc tgtccgttaa aatacggcca
    34021 tcttctgtca ctttgtcgcc gttgacgctc atcacaatga catctttatc tttcagcgcg
    34081 ccaataaata atttttgctg ccactggggg aatttatcgc tgttatagaa ggccatgccg
    34141 ctcacagcgg gcgaatcttt ccagtaaaaa acaggttgct cggtcccggc gacgatctcc
    34201 cctttcgctt ccggtatctt aaagcctgaa tagttgattc cccaggttgc cagcggccag
    34261 ccgtagtttt tgcctttttg cgggatatta atttcatcac caccgcgcgg gccatgttca
    34321 ttcagccaca gtgcattact ccacggattc atcgccattc cttgcggatt acgaatgcca
    34381 taagaccaga tctcggcgcg cgcaccggat tcctttataa aaggattatc atccgggatt
    34441 tcgccctggt cggtcagacg caccagtttg ccctgtaatt tatccagatc ctgcgccgtc
    34501 gggcgctgat tgttttcgcc cagagcaata aaaagataac ctttaccgtc gaataccagc
    34561 cgcccgccaa aatggttgcc ggtagacagt tttggcatct ggcgaaagac ggtgcggaag
    34621 tcggtcactt ttgagagatc atcacttaag cggccataac ccacagcagt tccggctttg
    34681 ccatcatcgc caacttcgga ataacttaac cagatgcggc gagactgagc aaaatcaggc
    34741 gctaaaacca cgtccagcag gccgccctgc ccgtgcgccc aaacgtccgg aactccggaa
    34801 agcggcgcag ataatccttt tcctgcttgc cagtgacgca actcgccgcc gcgcagagtg
    34861 attaacatac cgtgattatc gggtaaaaag gccagtgccc agggatggtc gagtttgtct
    34921 tgcagtactt cgacatttac cgttgcagga gccgcccaga gagcggaaga aagacaaata
    34981 aggggcacaa ggaaaaagga ttgtcgatgc ataacgcctc cggattgact ctggcttaaa
    35041 gcgtagtcag tggaggagat aacaaattca tttttacaaa aacttaaaca tgaaggggga
    35101 gacgctttct cccccttagt tttcaggcct tctcaagcat ggcgtgcttc tgcaggctct
    35161 ggatactcag cgttaagctc atcagacaat tttcaagctt atcggcgttg acggtaataa
    35221 cagtcgggca atcatggtgc ccactcatca aacatactgc ggctgtcgct aatgcttctt
    35281 cagcatgatg aagagcactc cactcttcct gatccagatg aagattcaac cgcagcgatt
    35341 tatcgtgcag ttcgcgattc agtttaaaaa agttatctcg tagatgattg ctttcgctga
    35401 cggacatgta tccttttgcc ttcctcagtt gcacgtatgc agcgaggtca acgcgtgcgt
    35461 cgatcaaccg gttcagcaga tcgattcgct gtctgtcaac gaacatactt cgatcctcct
    35521 ctttccagcc ttgttgcaat taaatagtat ggctattttt tgagcaaaat gctgtccagg
    35581 atcaattctt cacgccgaat taacaaaatt tacatttcct tcgcctccgc cagcgaatga
    35641 gattgcgata caaattttat ccagaactct gtagaatccc tgccctgacc tttcctgtaa
    35701 ctgaatattt tttaagctat gagcaaagta actccccagc cgaaaatcgg ctttgtttcc
    35761 cttggctgtc cgaaaaacct tgtcgattca gagcgtattc tcaccgaact ccgcactgaa
    35821 ggttatgacg tggtaccgag ctatgacgat gcggacatgg tgatcgtcaa cacctgcggc
    35881 tttattgaca gcgcggtaca agaatcactg gaagccattg gtgaagcgtt gaatgaaaac
    35941 ggcaaggtaa ttgtgaccgg ttgtctgggg gcaaaagaag atcagatccg cgaagtccac
    36001 ccgaaagtgc tggaaatcac cgggcctcat agctatgagc aggttctgga gcacgttcat
    36061 cactacgtgc caaaaccgaa acacaaccca ttcctgagcc tggtgccaga acaaggtgtg
    36121 aaactgacgc cgcgtcatta tgcctatctg aaaatttcgg aaggctgtaa tcaccgctgc
    36181 accttctgca ttattccgtc tatgcgcggc gacctggtga gccgtccgat tggcgaagtg
    36241 ttaagtgaag cgaaacgtct ggtagatgcg ggcgttaaag agattctggt gatctcgcag
    36301 gatacttccg cctatggcgt tgatgttaaa catcgtactg gcttccacaa cggcgagccg
    36361 gtaaaaacca gcatggtcag cctgtgcgaa cagttatcga aactggggat ctggacacgt
    36421 ctgcactacg tttaccctta tccgcatgtg gacgacgtca tcccactgat ggcagaaggc
    36481 aaaatcctgc cgtatctgga cattccgttg cagcacgcca gcccgcgcat tctcaaactg
    36541 atgaagcgtc cgggttctgt agatcgccaa ctggcgcgca tcaaacagtg gcgcgaaatc
    36601 tgcccggaac tgaccctacg ctcaaccttt attgtcggct tccctggcga gacagaagaa
    36661 gatttccaga tgctactcga cttcctgaaa gaagcgcgtc tggatcgcgt tggctgcttt
    36721 aaatacagcc cggttgaagg tgcagacgcc aatgccctgc ctgaccaggt tccggaagaa
    36781 gttaaagaag aacgctggaa ccgtttcatg cagctgcagc agcagatttc cgccgagcgc
    36841 ctgcaagaga aagtgggccg tgaaattctg gtgattatcg acgaagtgga cgaagaaggc
    36901 gcgattggtc gcagcatggc agatgcaccg gaaatcgacg gcgcggttta tctcaatggt
    36961 gaaaccaacg ttaagccggg tgatatcctg cgtgtgaaag tcgagcacgc cgatgagtac
    37021 gatttgtggg gtagccgggt ttaattaatc taaaggcaga tctaacaagg atattgttct
    37081 tcgatctgcc tctactaccc ttcccgatgt cctaccctta cgcacaaaat cccctgacgc
    37141 ataaatcgag aaaaatgaat cgtactcgag gcaactctca ctttttcaca ccaaattaaa
    37201 gttttgatcg caattacttg aaataagcgt aatggttact atagtaaaca ttaccacaag
    37261 gatggatgta tgggtgtgta taaggcaagg cgattcagcc aaagcacgaa aaaacttggt
    37321 attcacgata aagttctcat ggctgccgct gaagaagtga tgcaaggcat atgggaggct
    37381 gatttaggta gcggtgtcat caaaaaacgg ttacccctcc aacaaggaaa gagtggtgga
    37441 gccagaacaa ttatcttttt taaatcagca aatcatgtgt ttttttatga tggttggtcc
    37501 aagagtggtc taagcagtaa aggaaccaaa gaaatcgaag atgatgaact ggcagcctac
    37561 aaaaaaatgg caaatgcatt tctggccttt agcaataagc aaatagagga tttgatcgaa
    37621 actggcttct tgattgaggt gaaaaatgag cgataaatta caaatgatgc tggaagatgc
    37681 aaaggaactg aatgcattgg gcctgatgtc tgatagtgag gttgatgcca ttgccgtcat
    37741 ggtcaaagcc cgtgaagtca gcgcccgtat agccaaagtc aaatcaatga cgggtgaaga
    37801 gatcaaagcc atccgcaccc gatacggcat gtcacaatcc gtgctggcac atacaatggg
    37861 aatgtcaaaa gaaagtgtct cgaaatggga acgtaatgaa ataaaaccga gcggcccggc
    37921 actacgtatt ttgaatacat tggcaataaa agggccggaa gtttttgcaa agtaaaatgt
    37981 ttgcaaatga tttaccacat tcattagtac cccttgattc aggggtacta acactattct
    38041 aattctactg ccagctaatc tgagccctgc cgtttttctt actcttatac agtcgctcat
    38101 ccgccagcat tttcaagcgt tcgacggtag tacattcatt tgaatgcgct acaccgatac
    38161 tggtgcttaa attaatgctt cccacctgga aatgaacgat ttcttttagt atcgatagca
    38221 actcttccag cgtgccgcca gtataaatca ccgcaaattc gtcaccgccg aagcggtaga
    38281 ctttaccttt atctaccggg atctgtgatt taaggatttt caccactttc atcaacactt
    38341 catcgcccag caaatgaccg aaggtgtcgt taatattttt gaatttatcg acgtcaaaca
    38401 ggcacaaata accgcctttt tccggcagcg cttttaaatc ttcgtcaaag gattttctgc
    38461 ccattgcttt ggtcaggtcg tccaggaacg ccgagttata catactgtag agttttttga
    38521 catcggcagc cgaaaagaaa agcccgccat ccagtacgcg ttcaaaatgg ttattaataa
    38581 tggcttcttt ctccacaata ccacgattaa tcactctgcg taccagatag aggcaactgg
    38641 cacccgatat cactgccacc aatgcggcaa agaaaatgat caatgtacaa acccgcaaat
    38701 aaacatcttt tttatacgca aatacgtaca atccggtcgg caaataggca aagtgctcca
    38761 tcgcttccga aacggtgaaa atggtgtttt tagcattaaa ttcatcaccg gtcaccggtt
    38821 taccgttctt aaacagagag aacatcaggt tctcgctatc catcgccaga gattttgaca
    38881 gtaaagaaaa acgctccaga ccaagcaaaa atatcagact acgattgtga gttaccggat
    38941 agaggtaata gacgaaggtt ttaccgtcaa aagagataaa ccctgtattt ttgacatgtc
    39001 gatacttcac aacctgcata atatctgcac aataattttt ctcgaactcc tttacctgaa
    39061 tacattcgtc actggaaatc agataaggac cgacatcgta caagtaagag ggtgaaaccg
    39121 agtcagcgta agctgtgcgt aagccaacca acagctgttc ctccgtggga tttagcgtgt
    39181 tgataagatc aattgccacg ctacgccgat attcagcaat atttgccacc gcaaaagcag
    39241 tatcaataac ggactgtttc aggccataca cccgttcctt taccatttga gtgtagatcc
    39301 cgcaggcaac tgctgaaatc atgataaaaa tcagcagact gactgtaagt acgaacttat
    39361 tgattctgga catacgtaaa ctactctttt actaattttc cacttttatc ccaggcggag
    39421 aatggcatgg gtttctggta cagatacccc tgagcctgaa cacatcctaa tttgattaat
    39481 tcgcgcgcaa cgtcttcgct ctctacgcct tccgcaatca ccgggatccg gttatagcgt
    39541 gccaggtcga aaataatgcg cacaattttt cccgcagtac cgaaaatatc gccggtaaat
    39601 tgcgagtcca cctttatgct atctggatta atttcataaa aacgtttcag gtttgacaaa
    39661 ccggtgccaa agtcatcaat cgatatccgt accccttgtt tacgcagttg ttcaatattg
    39721 agataaaaat gttcattaat tcgctcgatc acggtttcag tgatctcaac acagaggctg
    39781 ttcgccttca ggccattttc tttcatgatg gtggtaaaac gctcatgaaa gtgcggttcg
    39841 tttaactgcc atggcgaaat attaatatgc agtaagaaat catccttcag cgacgcacgc
    39901 tgactaaaat gtctgaattc acccattgcc gttttaataa tctgatagcc cagctcattg
    39961 ataaacccga tatcttcagc aatggtaatg aacttcagcg gtgaaatgat cccccgctcc
    40021 gcagacaccc aacggcacaa cgcctccgcg cccagaatgg cgcgggtgtt aatatcaacg
    40081 atcggttgat agaccaggaa tagttcgcca atttccattg catggcgaat attacgcgca
    40141 atccagatat tctcttctat ttcgttgagt ttcgcgtcat cacagaaaat aaactctgag
    40201 aacatatggt cacggatact ggaaatcgcc agtttcagtt tttctgcgta actccaggcg
    40261 ttttccccct gcaaaggttc acaaatggca gacttatgga tgcgcagatt acggtagcca
    40321 aagctgctgt caaaatcgtt taccatcgct attttacggg taaatgtctg tacattttcc
    40381 cggcacagaa gtaaatagag atcggcattg aatgtcacga tatcgcagca ttgcgcatat
    40441 ttttcggcaa ttcgctgaca caatttcctg acgattttat tgcgttcatc aacaccaaac
    40501 accgcttcca cggcttccag accacacagt ttgatgatcc ccacagccag atactgatta
    40561 ttgttggact caataatcgc catattttta ataaaaccgc tttcctgatc gtaagagatc
    40621 agtgagagca atttatttga ccgttcaatt tcggcatctt tcatttccgc gatcgaattt
    40681 ttaatgctga taatctcttt cgcatcgcct tcgcgaattt tcagagcctc gatttgatcc
    40741 gttgagtcgg gcaaaaatgc cagagtatta accagctctg tcatttcaga aaattgccga
    40801 cggaacacac gagtggtcat taaataaacc accagcaata cgataataat gaacgcaaac
    40861 ggcagataaa aaagtttatc tgaggcgttc aataaattac cgccaggata ataaaaatag
    40921 atttcgaaca atgcattatt tggcactgct gatgtctttg caatatactc cgtatcgaca
    40981 gaatcaataa tattataatt accttcgcta ttataaataa tcagtttttc acgagcgagt
    41041 tgattcttgg caatgatctc atgaccttgt ttcaaaaata gaacttcatc attagcgctg
    41101 atttgatcaa gatactcgtt cagataccgc cagtttaaat cgacatgaat tttatctacc
    41161 gctaacaaat cgtgatcttt gccgttgata cgtagcgtaa aagagataca ttttttaccc
    41221 ggacgatcaa agcaattgta tgccggattc cagctgttgt tagagtcatc tgaagccacc
    41281 gaccacggtc gtttggatgg gttaaaatcg gcaaaaataa cccgccctga ctcgtcgaca
    41341 agcggtccat ttacttgttc ctgatgataa acaacgattt tatcattcgc cctccgtgaa
    41401 aagagataat atttattatc atcaagtacc agtcccaccg agtctaaaaa tggcatcatt
    41461 tccattttca gaatcacggc gcgttgaatc tcctctgtat tttttaataa atacagtgag
    41521 tcctt
//
LOCUS       DAPHBJ010000016        40568 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.016, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000016 DAPHBJ010000000
VERSION     DAPHBJ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.016"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            332..418
                     /gene="leuL"
                     /locus_tag="RHZ76_000815"
     CDS             332..418
                     /gene="leuL"
                     /locus_tag="RHZ76_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HDV2811507.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            511..2082
                     /gene="leuA"
                     /locus_tag="RHZ76_000816"
     CDS             511..2082
                     /gene="leuA"
                     /locus_tag="RHZ76_000816"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDV2811508.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2082..3173
                     /gene="leuB"
                     /locus_tag="RHZ76_000817"
     CDS             2082..3173
                     /gene="leuB"
                     /locus_tag="RHZ76_000817"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDV2811509.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTNHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3176..4576
                     /gene="leuC"
                     /locus_tag="RHZ76_000818"
     CDS             3176..4576
                     /gene="leuC"
                     /locus_tag="RHZ76_000818"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDV2811510.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAIAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4587..5192
                     /gene="leuD"
                     /locus_tag="RHZ76_000819"
     CDS             4587..5192
                     /gene="leuD"
                     /locus_tag="RHZ76_000819"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDV2811511.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5241..6419)
                     /gene="setA"
                     /locus_tag="RHZ76_000820"
     CDS             complement(5241..6419)
                     /gene="setA"
                     /locus_tag="RHZ76_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HDV2811512.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLILFHSRLALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWIIAVISVVALFLTAKVKDI
                     "
     gene            complement(6521..6652)
                     /gene="sgrT"
                     /locus_tag="RHZ76_000821"
     CDS             complement(6521..6652)
                     /gene="sgrT"
                     /locus_tag="RHZ76_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HDV2811513.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6741..8396
                     /gene="sgrR"
                     /locus_tag="RHZ76_000822"
     CDS             6741..8396
                     /gene="sgrR"
                     /locus_tag="RHZ76_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HDV2811514.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEIDYDQWHTGEIES
                     DIWLNSANFTLPLDFSVFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLLHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8430..8529
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8560..9543
                     /gene="thiB"
                     /locus_tag="RHZ76_000823"
     CDS             8560..9543
                     /gene="thiB"
                     /locus_tag="RHZ76_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HDV2811515.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAINVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDTPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9519..11129
                     /gene="thiP"
                     /locus_tag="RHZ76_000824"
     CDS             9519..11129
                     /gene="thiP"
                     /locus_tag="RHZ76_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HDV2811516.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AIWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFIEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGLNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11113..11811
                     /gene="thiQ"
                     /locus_tag="RHZ76_000825"
     CDS             11113..11811
                     /gene="thiQ"
                     /locus_tag="RHZ76_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HDV2811517.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11925..12689)
                     /locus_tag="RHZ76_000826"
     CDS             complement(11925..12689)
                     /locus_tag="RHZ76_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV2811518.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12775..13653)
                     /gene="araC"
                     /locus_tag="RHZ76_000827"
     CDS             complement(12775..13653)
                     /gene="araC"
                     /locus_tag="RHZ76_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HDV2811519.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13992..15692
                     /gene="araB"
                     /locus_tag="RHZ76_000828"
     CDS             13992..15692
                     /gene="araB"
                     /locus_tag="RHZ76_000828"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HDV2811520.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLSWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRSPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15703..17205
                     /gene="araA"
                     /locus_tag="RHZ76_000829"
     CDS             15703..17205
                     /gene="araA"
                     /locus_tag="RHZ76_000829"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDV2811521.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHADHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17490..18185
                     /gene="araD"
                     /locus_tag="RHZ76_000830"
     CDS             17490..18185
                     /gene="araD"
                     /locus_tag="RHZ76_000830"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDV2811522.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18260..20611
                     /gene="polB"
                     /locus_tag="RHZ76_000831"
     CDS             18260..20611
                     /gene="polB"
                     /locus_tag="RHZ76_000831"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HDV2811523.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGIAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHAEEEAAKIG
                     SALVQHVNAWWAETLQQQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20776..23682
                     /gene="rapA"
                     /locus_tag="RHZ76_000832"
     CDS             20776..23682
                     /gene="rapA"
                     /locus_tag="RHZ76_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HDV2811524.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESCVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23694..24353
                     /gene="rluA"
                     /locus_tag="RHZ76_000833"
     CDS             23694..24353
                     /gene="rluA"
                     /locus_tag="RHZ76_000833"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HDV2811525.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24470..25285)
                     /gene="djlA"
                     /locus_tag="RHZ76_000834"
     CDS             complement(24470..25285)
                     /gene="djlA"
                     /locus_tag="RHZ76_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HDV2811526.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQTGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25540..27894
                     /gene="lptD"
                     /locus_tag="RHZ76_000835"
     CDS             25540..27894
                     /gene="lptD"
                     /locus_tag="RHZ76_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HDV2811527.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27947..29233
                     /gene="surA"
                     /locus_tag="RHZ76_000836"
     CDS             27947..29233
                     /gene="surA"
                     /locus_tag="RHZ76_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HDV2811528.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29233..30222
                     /gene="pdxA"
                     /locus_tag="RHZ76_000837"
     CDS             29233..30222
                     /gene="pdxA"
                     /locus_tag="RHZ76_000837"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDV2811529.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30219..31040
                     /gene="rsmA"
                     /locus_tag="RHZ76_000838"
     CDS             30219..31040
                     /gene="rsmA"
                     /locus_tag="RHZ76_000838"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDV2811530.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            31043..31420
                     /gene="apaG"
                     /locus_tag="RHZ76_000839"
     CDS             31043..31420
                     /gene="apaG"
                     /locus_tag="RHZ76_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HDV2811531.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31427..32269
                     /gene="apaH"
                     /locus_tag="RHZ76_000840"
     CDS             31427..32269
                     /gene="apaH"
                     /locus_tag="RHZ76_000840"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HDV2811532.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32347..32826)
                     /gene="folA"
                     /locus_tag="RHZ76_000841"
     CDS             complement(32347..32826)
                     /gene="folA"
                     /locus_tag="RHZ76_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HDV2811533.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(33018..34880)
                     /gene="kefC"
                     /locus_tag="RHZ76_000842"
     CDS             complement(33018..34880)
                     /gene="kefC"
                     /locus_tag="RHZ76_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HDV2811534.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGCGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34873..35403)
                     /gene="kefF"
                     /locus_tag="RHZ76_000843"
     CDS             complement(34873..35403)
                     /gene="kefF"
                     /locus_tag="RHZ76_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HDV2811535.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35511..36842)
                     /locus_tag="RHZ76_000844"
     CDS             complement(35511..36842)
                     /locus_tag="RHZ76_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2811536.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(36899..37186)
                     /gene="fixX"
                     /locus_tag="RHZ76_000845"
     CDS             complement(36899..37186)
                     /gene="fixX"
                     /locus_tag="RHZ76_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HDV2811537.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(37183..38469)
                     /locus_tag="RHZ76_000846"
     CDS             complement(37183..38469)
                     /locus_tag="RHZ76_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDV2811538.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38520..39461)
                     /locus_tag="RHZ76_000847"
     CDS             complement(38520..39461)
                     /locus_tag="RHZ76_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDV2811539.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(39476..40246)
                     /locus_tag="RHZ76_000848"
     CDS             complement(39476..40246)
                     /locus_tag="RHZ76_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HDV2811540.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         9798 a        11191 c        10620 g         8959 t
ORIGIN      
        1 tcaataatat tcaccaaatc aatatcaaaa aaaatcgcaa aacatataat tcaatacaaa
       61 tcatcaggat aggttttgca acgcgtgcat tttgtcccct ttttcctcgt tgattagatg
      121 caaaaattta tgctgaaata tgtcaaccga tgaaaagcgt cggtagttaa gcagaaatta
      181 atatcgctta ctttaaccac cgcagcacaa ttagctaatt ttacggatgc agaactcacg
      241 ctggcgggac gtttttattg cgtcagggtt gacatccgtt tttgtatcca gtaactctaa
      301 aagcatatcg cattcatctg gagctgattt aatgactcac atcgttcgct ttatcggtct
      361 actactacta aacgcatctt ctttgcgcgg tagacgagtg agcggcatcc agcattaagc
      421 cagcacgcag tcaaacaaaa aacccgcgcc attgcgcggg tttttttatg cccgaagcga
      481 ggcgctctaa aagagacaag gacccaaacc atgagccagc aagtcattat tttcgatacc
      541 acattgcgcg acggtgaaca ggcgttacag gcaagcttga gtgtgaaaga aaaactgcaa
      601 attgcgctgg cccttgagcg tatgggtgtt gacgtgatgg aagtcgggtt ccccgtttct
      661 tcgccgggtg attttgaatc agtgcaaacc atcgctcgcc aggttaaaaa cagccgcgta
      721 tgcgcgttag ctcgctgcgt ggaaaaagat atcgacgtgg cggccgaatc cctgaaagtc
      781 gccgaagcct tccgtattca tacctttatt gccacttcgc caatgcacat cgccaccaag
      841 ctgcgcagca cgctggacga ggtgatcgaa cgcgctatct atatggtgaa acgcgcccgt
      901 aattacaccg atgatgttga attttcttgc gaagatgccg ggcgtacacc cattgccgat
      961 ctggcgcgag tggtcgaagc ggcgattaat gccggtgcca ccaccatcaa cattccggac
     1021 accgtgggct acaccatgcc gtttgagttc gccggaatca tcagcggcct gtatgaacgc
     1081 gtgcctaaca tcgacaaagc cattatctcc gtacataccc acgacgattt gggcctggcg
     1141 gtcggaaact cactggcggc ggtacatgcc ggtgcacgcc aggtggaagg cgcaatgaac
     1201 gggatcggcg agcgtgccgg aaactgttcc ctggaagaag tcatcatggc gatcaaagtt
     1261 cgtaaggata ttctcaacgt ccacaccgcc attaatcacc aggagatatg gcgcaccagc
     1321 cagttagtta gccagatttg taatatgccg atcccggcaa acaaagccat tgttggcagc
     1381 ggcgcattcg cacactcctc cggtatccac caggatggcg tgctgaaaaa ccgcgaaaac
     1441 tacgaaatca tgacaccaga atctattggt ctgaaccaaa tccagctgaa tctgacctct
     1501 cgttcggggc gtgcggcggt gaaacatcgc atggatgaga tggggtataa agaaagtgaa
     1561 tataatttag acaatttgta cgacgctttc ctgaagctgg cggacaaaaa aggtcaggtg
     1621 tttgattacg atctggaggc gctggccttc atcggtaagc agcaagaaga gccggagcat
     1681 ttccgtctgg attacttcag cgtgcagtct ggctctaacg atatcgccac cgccgccgtc
     1741 aaactggcct gcggcgaaga agtcaaagca gaagccgcca acggtaacgg tccggtcgat
     1801 gccgtctatc aggcgataaa ccgcatcact gactataacg tcgaactggt gaaatacagc
     1861 ctgactgcta aaggtcacgg taaagatgct ctgggtcagg tggatattgt cgctaactac
     1921 aacggtcgcc gcttccacgg cgtcggcctg gccaccgata ttgtcgagtc ctccgccaaa
     1981 gccatggtgc acgtactgaa caatatctgg cgcgccgcag aagtcgaaaa agagttgcaa
     2041 cgcaaagctc aacacaacga aaacaacaag gaaaccgtgt gatgtcgaag aattaccata
     2101 ttgccgtatt gccgggggac ggtattggtc cggaagtgat gacccaggcg ctgaaagtgc
     2161 tggatgccgt gcgcaaccgc tttgcgatgc gcatcaccac caaccattac gatgtaggcg
     2221 gcgcagccat tgataaccac gggcagccgc tgccgcctgc gacggttgaa ggttgtgagc
     2281 aagccgatgc cgtgctgttt ggctcggtag gcggtccgaa atgggaacat ttaccaccag
     2341 accagcaacc agaacgcggc gcgctgctgc ctctgcgtaa gcacttcaaa ttattcagca
     2401 acctgcgccc ggcaaaactg tatcaggggc tggaagcatt ctgtccgctg cgtgcagaca
     2461 ttgccgcaaa cggcttcgac atcctgtgtg tgcgcgaact gaccggcggc atctatttcg
     2521 gtcagccaaa aggccgcgaa ggtagcggac aatatgaaaa agcctttgat accgaggtgt
     2581 atcaccgttt tgagatcgaa cgtatcgccc gcatcgcgtt tgaatctgct cgcaagcgtc
     2641 gccacaaagt gacgtcgatc gataaagcca acgtgctgca atcctctatt ttatggcggg
     2701 agatcgttaa cgagatcgcc acggaatacc cggatgtcga actggcgcat atgtacatcg
     2761 acaacgccac catgcagctg attaaagatc catcacagtt tgacgttctg ctgtgctcca
     2821 acctgtttgg cgacattctg tctgacgagt gcgcaatgat cactggctcg atggggatgt
     2881 tgccttccgc cagcctgaac gagcaaggtt ttggtctgta tgaaccggca ggcggctcag
     2941 caccagatat cgcaggcaaa aacatcgcca acccgattgc gcaaattctg tcgctggcac
     3001 tgctgctgcg ctacagcctg gatgccgatg atgcggcttg cgccattgaa cgcgccatta
     3061 accgcgcatt agaagaaggc attcgcaccg gggatttagc ccgtggcgct gccgccgtta
     3121 gtaccgatga aatgggcgat atcattgccc gctatgtggc agaaggggtg taatcatggc
     3181 taaaacgtta tacgaaaaat tgttcgacgc tcacgttgtg tacgaagccg aaaacgaaac
     3241 cccactgtta tatatcgacc gccacctggt gcatgaagtg acctcaccgc aggcgttcga
     3301 tggtctgcgc gcccacggtc gcccggtacg tcagccgggc aaaaccttcg ctaccatgga
     3361 tcacaacgtc tctacccaga ccaaagacat taatgcctgc ggtgaaatgg cgcgtatcca
     3421 gatgcaggaa ctgatcaaaa actgcaaaga atttggcgtc gaactgtatg acctgaatca
     3481 cccgtatcag gggatcgtcc acgtaatggg gccggaacag ggcgtcacct tgccggggat
     3541 gaccattgtc tgcggcgact cgcataccgc cacccacggc gcgtttggcg cactggcctt
     3601 tggtatcggc acttccgaag ttgaacacgt actggcaacg caaaccctga aacagggccg
     3661 cgcaaaaacc atgaaaattg aagtccaggg caaagccgcg ccgggcatta ccgcaaaaga
     3721 tatcgtgctg gcaattatcg gtaaaaccgg tagcgcaggc ggcaccgggc atgtggtgga
     3781 gttttgcggc gaagcaatcc gtgatttaag catggaaggt cgtatgaccc tgtgcaatat
     3841 ggcaatcgaa atgggcgcaa aagccggtct ggttgcaccg gacgaaacca cctttaacta
     3901 tgtcaaaggc cgtctgcatg cgccgaaagg caaagatttc gacgacgcca ttgcctactg
     3961 gaaaaccctg caaaccgacg aaggcgcaac tttcgatacc gttgtcactc tgcaagcaga
     4021 agaaatttca ccgcaggtca cctggggcac caatcccggc caggtgattt ccgtgaacga
     4081 caatattccc gatccggctt cgtttgccga tccggttgaa cgtgcgtcgg cagaaaaagc
     4141 gctggcctat atggggctga aaccgggtat tccgctgacc gaagtggcta tcgacaaagt
     4201 gtttatcggt tcctgtacca actcgcgcat tgaagattta cgcgcggcag cggagatcgc
     4261 caaagggcga aaagtcgcgc caggcgtgca ggcactggtg gttcccggct ctggcccggt
     4321 aaaagcccag gcggaagcgg aaggtctgga taaaatcttt attgaagccg gttttgaatg
     4381 gcgcttacct ggctgctcaa tgtgtctggc gatgaacaac gaccggctga atccgggcga
     4441 acgttgtgcc tccaccagca accgtaactt tgaaggccgc caggggcgcg gcgggcgcac
     4501 gcatctggtc agcccggcaa tggctgccgc cgcggctgtg accggccatt tcgccgacat
     4561 tcgcaacatt aaataaggag cacaccatgg cagagaaatt tatcaaacac acaggcctgg
     4621 tggttccgct ggatgccgcc aatgtcgata ccgatgcaat catcccgaaa cagtttttgc
     4681 agaaagtgac ccgtactggt tttggcgcgc atctgtttaa cgactggcga tttctggatg
     4741 aaaaaggcca acagccaaac ccggacttcg tgctgaactt cccgcagtat cagggcgctt
     4801 ccattttgct ggcacgagaa aacttcggct gtggctcctc gcgtgagcac gcgccctggg
     4861 cattgaccga ctacggtttt aaagtggtga ttgcgccgag ttttgctgac atcttctacg
     4921 gcaatagctt taacaaccag ctgctgccgg tgaaattaag cgatgcagaa gtggacgaac
     4981 tgtttgcgct ggtgaaagcc aatccgggga tccatttcga cgtggatctg gaagcgcaag
     5041 aggtgaaagc gggagagaaa acctatcgct ttaccataga tgccttccgc cgccactgca
     5101 tgatgaacgg tctggacagt attgggctta ccttgcagca cgacgacgcc attgccgctt
     5161 atgaagcaaa acaacctgcg tttatgaatt aatccccttg cccggtcaaa tgaccgggct
     5221 ttccgctatc gtccacgtca tcaaatgtct ttaacctttg cggttaaaaa taatgcgaca
     5281 acagaaataa ccgcaattat ccagtagaca gcaaagtgcc cccaactttg tgcaattgct
     5341 ccctgaataa cgccagccag aattaccccg gtagaaatac tgttagtaaa taaggtggta
     5401 gctgcccccg ctcttccagg cattaaatcc tgaaaccata gcatcccaat acccgcaaca
     5461 atgccgataa atacagcgtt aaaaagttgc agcgtcatca acgccagacg gctatgaaat
     5521 aaaatcaatc cggtgtaaaa cagtactcct gccgccactg ctatgaccat cattcgccgc
     5581 ttaccataac gtttgacata gtagccagcc agaatcattg ctggtatttc cagtccagct
     5641 gccgtcccca tcaggaaacc cgccagtttg tctggcaatc ctaactcgct actgatccac
     5701 aacggcatat caataatgta catggtgttg caggtccaca ttaacgtcga ggcgacaaat
     5761 aacatccgta cgttactatc ctgccagcca ccttgcattg ataaagcatt ttccgacggc
     5821 agttctaccc gcgccacaga cggaagcata aatgcaatca ataccagact gagtgtgaat
     5881 atcccggcgg caatcgaaaa catcaccgta aagccgtaat tcaacgccag cataaaggcc
     5941 aacggtggac cgataaccca tgccagagaa agctgcgcac gcatcaccga gctaaacatc
     6001 accacttctc gcgccgagtt atccgcatat tcccgcgcca gagcaaataa ctgtggcatt
     6061 gccgtattgg ccagagatgc cagaagcaca ccacaggtga taagcgtcag ataatgacga
     6121 ttaaatgcaa acaatagcgc attgccgata gccatcaaac agcaaaatat aatcagtttt
     6181 cgccgatcgc cctgactgtc agaacgtttt gccaaccaga ggcttacgcc gatcccagca
     6241 atagcattca ccgtataaaa gagtccgatc cagaaaggtt gcgcgccaac ctcacgactc
     6301 agaaataagc tcaatgtagg agcctgtagc gccccggcca cccccatcat aaaagcgacc
     6361 agcataaatg ccgcgtaaac accgttcata cggcgagcca tcgtcattat ccagatcata
     6421 cgttcccttt ttagcgcggc gagaataaaa aaaaccagca ggtataatct gctggcgggt
     6481 gattttacac caatactcag tcacacatga tgcaggcaag tcaactttca gaattgcggt
     6541 catcccactg catcagtcct tcaagcatgg ttaatcgttg tgggacgctt aaccaacgca
     6601 accagcacaa cttcgctgtc gcggtaaaat agtgctgata aaactgacgc atggggcacc
     6661 tccttgcttc atcgttagga attattgact taatataggg aaaataaaat tgctgtcttt
     6721 tgcgcaggag ttcccctttt atgccatctg ctcgtctgca acaacagttc atccgcctgt
     6781 ggcaatgctg cgagggtaaa tcgcaggaca caacgctcaa cgaactggca gcgttattga
     6841 gctgctcgcg tcgtcatatg cgtaccctgc tcaacaccat gcaggatcgc ggctggctga
     6901 cgtgggaagc ggaagtcggg cgcggtaaac gctcgcgtct gacattcctc tataccgggc
     6961 tggcgcttca gcaacagcgg gcggaagacc tgctggagca ggatcgtatc gaccaactag
     7021 tacagttggt tggcgacaaa gcgactgtgc ggcaaatgct ggtttctcat ctgggccgca
     7081 gcttccgcca ggggcggcac atcctgcgcg tgctctacta tcgtccgttg cgtaatctgc
     7141 tacctggcag cgcattgcgc cgttccgaaa cccatattgc ccggcaaatc ttcagttcgc
     7201 taacgcgcat aaatgaggaa aatggggaac tggaagcaga catcgcccac cactggcagc
     7261 aaatatcacc gcttcactgg cgtttctttt tgcgtccagg agtccatttt catcatggtc
     7321 gtgaactgga aatggacgac gtgatcgcct ctttaaaacg aatcaatacg ctgccgctct
     7381 attcgcatat tgctgacatt gtgtcgccga cgccctggac gctggatatc catctcacgc
     7441 aaccggaccg ctggttaccg ttattgctgg ggcaagttcc ggcgatgatc ctgccgcgcg
     7501 aatgggaaac cctcagtaac tttgccagcc atcccatcgg caccggtccg tatgcggtga
     7561 tccgcaacag caccaatcaa ctgaaaattc aggcattcga tgacttcttc ggttaccggg
     7621 cattaatcga cgaagttaac gtctgggttc tgccggaaat tgccgacgag ccagccggag
     7681 gtctgatgct aaaaggtcca cagggcgagg aaaaagagat tgaaagccgc ctggaggaag
     7741 gttgctacta tttactattc gacagccgca cccatcgcgg ggcgaatcag caagtcaggg
     7801 actgggtaag ctatgtgctt tctccaacta atctggtcta tttcgctgag gaacagtacc
     7861 agcaactgtg gttcccggct tatggactgc tcccccgttg gcaccatgcc cgcaccataa
     7921 agagcgaaaa accggctggt ctggaaagcc tcaccctaac cttttatcag gatcacagtg
     7981 agcatcgggt gattgccggg atcatgcagc agattctggc aagtcaccag gtcacgctgg
     8041 aaatcaaaga gatcgactac gatcagtggc atacaggaga gatcgaaagt gatatctggc
     8101 taaacagcgc caactttacc ctgccgctgg acttctctgt tttcgcacat ttatgcgaag
     8161 tgccactgct acaacattgc attcccattg actggcaagc cgacgctgct cgctggcgca
     8221 atggcgagat gaatctggcg aactggtgcc agcaactggt cgccagcaaa gcgatggtgc
     8281 cattattgca ccactggctg atcattcagg ggcaacgcag tatgcgcggc ctgcgcatga
     8341 acaccctcgg ctggttcgat tttaaatcag cgtggtttgc gccaccggat ccatgattgc
     8401 tggttgataa caaaatcact acactaacgc cgttctcaac ggggtgccac gcaaacgcgt
     8461 gcgctgagaa aatacccgtc gaacctgatc cggataacgc cggcgaaggg atttgaggct
     8521 ccttctcaag tcctttgcca ctcttttttg aggtgcaaag tgttaaaaaa atgtctgccc
     8581 ctgctgttgc tgtgcacagc gcccgttttc gctaaacccg ttctgactgt ttatacctac
     8641 gattccttcg ccgccgactg ggggcctggt ccggtggtta aaaaagcctt tgaagccgac
     8701 tgtaattgcg aactgaaact ggtggcgctg gaagatggcg tttcgcttct caaccgtcta
     8761 cggatggaag gcaaaaacag caaagccgat gtagttctgg ggctggataa caacctgtta
     8821 gacgccgcca gtaaaaccgg actgtttgcc aaaagcggcg tggcagcgga tgccattaac
     8881 gttcccggcg gctggaataa tgacactttc gtaccgtttg attacggcta cttcgccttc
     8941 gtctacgaca agaacaaact gaaaaacccg ccccaaagcc tgaaagagct ggttgagagc
     9001 gatcaaaact ggcgagtgat ttatcaggat ccgcgcacca gtacgccggg gctgggtctg
     9061 ctgttgtgga tgcaaaaagt ctatggcgat gacaccccgc aggcctggca gaaactggcg
     9121 aagaaaactg tcaccgtcac caaaggctgg agcgaagcct acggcctgtt tttaaaaggt
     9181 gaaagcgatc tggtactgag ttacaccacc tctccggctt atcacattct cgaagagaaa
     9241 aaagataact acgccgccgc gaactttagc gaaggtcact atctgcaggt ggaagtcgcc
     9301 gcccgcaccg ctgccagcaa gcagccggag ctggcgcaaa aatttctcca gtttatggtt
     9361 tctccggctt tccagaatgc gatcccaacc ggcaactgga tgtatccggt ggcaaatgtc
     9421 acgctgcctg ccgggtttga acaattgacc aaaccagcaa ccacgctgga gttcacgcca
     9481 gccgaagtgg cggcacaacg tcaggcatgg attagcgaat ggcaacgcgc cgtcagccgt
     9541 taattcccgg ctggttaatt ccaggtgtaa gcgccgccac gctggtggta gcggttgcgc
     9601 tcgcggcgtt tctcgccctg tggtggaacg cgccgcaggg taactgggtg gcaatctggc
     9661 aggacagcta cctgtggcat gtggtgcgct tctccttctg gcaggcgttt ctctcggcgc
     9721 tgctctctgt cgtacccgcg atattcctcg cccgcgcact ctatcgcagg cgctttccgg
     9781 gtcggctggc gctgttgcgt ctgtgcgcaa tgaccttgat cctcccggtg ctggttgctg
     9841 ttttcggcat tcttagcgtc tatggtcgtc agggctggct ggcatcgctc tgccaatcgc
     9901 tcggtctgga gtggaccttt tcgccctacg gcctgcaagg tattttgctg gcgcacgtat
     9961 tttttaatct accgatggcg agccgcttat tactccaggc actggaaaac attcccggcg
    10021 agcaacgtca gcttgccgcc cagcttggga tgcgcggctg gcattttttc cgcttcatcg
    10081 aatggccgtg gctacggcga caaatcccgc cggttgccgc gcttatcttt atgctctgtt
    10141 tcgccagctt cgccaccgtg ctgtcgctgg gcggcggtcc gcaggcaacc actatcgagc
    10201 tggcaatcta tcaggcgctg agttacgact acgatcctgc ccgcgcggcg atgctggcgc
    10261 tgatccagat ggtgtgttgc ctcgggttgg tgctgctgag tcagcgattg agtaaggcca
    10321 ttgcgccagg caccacgctg ctgcaaggct ggcgcgaccc ggacgatcgt ctgcatagcc
    10381 gcatttgcga cacggtgtta attgtgctgg cgctgctgct gttgctgcca ccgttgctgg
    10441 cggtgatcgt cgatgggcta aatcgccagt tgccggaagt gctggcacaa ccggtgctgt
    10501 ggcaggcgct gtggacctcg ttgcgtattg cgctggcggc aggtgtattg tgcgtagtgc
    10561 tgaccatgat gctgctatgg agcagtcgcg aacttcgggc gcggcagaaa atgctggcgg
    10621 gtcaggcgct ggagatgagc ggcatgttga ttctcgccat gccggggatt gtgctggcta
    10681 ccggcttctt tttactgctc aacaacacca ttggcctgcc gcaatctgct gacggcattg
    10741 tgattttcac caatgcgtta atggcgatcc cttatgcgct gaaagtactg gaaaacccga
    10801 tgcgcgatat caccgcccgc tacagtatgt tgtgtcagtc gctgggcatt gaaggctggt
    10861 cgcgcttaaa agtggtcgag ctgcgcgccc tgaaacgtcc actggcgcag gcgctggctt
    10921 ttgcctgcgt gctgtcgatt ggtgattttg gcgtagtggc gttgttcggt aacgatgatt
    10981 tccgtaccct gccgttttat ctctaccagc aaattggctc ctatcgcagc caggacggtg
    11041 cggtcaccgc gttaattctg ctactactct gttttctgct gtttaccgtg attgaaaaac
    11101 taccggggcg aaatgttaaa actgactgat atcacctggc tttatcacca tttgccgatg
    11161 cgttttagct taacggtaga acgcggcgag caggtggcaa tcctcgggcc aagcggcgcg
    11221 ggtaaaagta ccctgctgaa tttgatcgcc ggttttctga cgccagccag cggttcgctg
    11281 actatcgacg gcgttgatca caccacgacg ccgccgtcac gccgtccggt gtcgatgctg
    11341 tttcaggaaa acaacctgtt cagtcacctg acggtcgcgc agaacatcgg gctggggcta
    11401 aatccgggat tgaaactgaa cgcggcacag caggagaaaa tgcacgctat cgcccgccag
    11461 atggggattg ataatttaat ggcgcggtta cctggcgagc tttccggcgg tcagcgacag
    11521 cgagtggcgt tagcacgttg tctggtacgc gaacagccga ttttattgct cgatgaaccg
    11581 ttctctgcgc tcgatccggc actacgccag gagatgttga cgctggtgag cacgagctgc
    11641 cagcagcaaa aaatgacgtt attgatggtg tcacacagtg tggaagatgc cgcgcggatc
    11701 gccacgcgct cggtggtggt cgccgacggg cgcatcgcct ggcagggtaa gaccaatgag
    11761 ttgttgagcg gtaaggcgag tgcttcagca ctattgggga ttacgggtta gtatgcactg
    11821 tcagatgcgg cgtgaacgcc ttatccgacc tacggacagt gcgttttgta ggcctgataa
    11881 gacgcggtca gcgtcgcatc aggcaacccg cacaagacaa tcccctaaac cccaaccact
    11941 ttacgcaaaa tatcgatata caccggcatc aacgggtggc gaattaacac caccagcgcc
    12001 accacgccga tggcagaaat caacggcgtc agccacaaca aacggccgcg cgacaaataa
    12061 tgacttaaac gatcggttgc tttaccgctg cgccataacc gccagcacag ccagccacca
    12121 acccacaaaa ataccgccgt cgccagcagc aaccatttaa actcgccgct ctgcattccg
    12181 gctggaatat cgatcgccgc gcccgccaga atcccaggca ggaagtaaaa cggtggccac
    12241 agcaggcagc cgataatatt cggcgtaatg aattttgcca ccggcagatc cagcattccc
    12301 gccaccattg gcaccagcgg acgcgtcggg ccaacaaagc gaccgaccag aatggtgaac
    12361 atgctgtgtt gatgcaacgc atgttcagtt ttatcgagta atgctttgtt tttcttcaga
    12421 aatgaccagc gatgcaacgg ctttttaaaa cgccagccca gccagaagga gatccagtcg
    12481 cccagcagac aaccgacaat ccctgccagc caggcgtgcc agaaacttaa ctcgccgctg
    12541 ccaatcagcg ctcccagccc cgccatcagc accgtaccgg gtagaatcaa accgaccagc
    12601 gccagcgact ccagaaaggc caccaacacc accgccatca atgaatacac ggtagattgg
    12661 gtaataaagt gttccagcaa tgcttgcata atgtgcctgt caaatggacg aagcagggat
    12721 tctgcaaacc ctatgctact ccgtcaagcc gtcaattgtc tgattcgtta ccaattatga
    12781 caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct
    12841 ggccccggtg cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt
    12901 gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat
    12961 acgttggtcc tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagatgtga
    13021 cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc
    13081 tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg
    13141 gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca gatttatcgc
    13201 cagcagctcc gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc
    13261 gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga
    13321 cggccagtta agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata
    13381 ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa
    13441 aatatcaccc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg
    13501 aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag
    13561 ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc
    13621 cggcagcagg ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagaga
    13681 agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt
    13741 ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa
    13801 agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga
    13861 ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga
    13921 tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccgt ttttttggat
    13981 ggagtgaaac gatggcgatt gcaattggcc tcgattttgg cagtgattct gtgcgagctt
    14041 tggcggtgga ctgcgccagc ggtgaagaga tcgccaccag cgtagagtgg tatccccgtt
    14101 ggcaaaaagg gcaattttgt gatgccccga ataaccagtt ccgtcatcat ccgcgtgact
    14161 acattgagtc aatggaagcg gcactgaaaa ccgtgcttgc agagcttagc gtcgaacagc
    14221 gcgcagctgt ggtcgggatt ggcgttgaca gtaccggctc gacgcccgca ccgattgatg
    14281 ccgacggtaa cgtgctggcg ctgcgcccgg agtttgccga aaacccgaac gcgatgttcg
    14341 tattgtggaa agaccacact gcggttgaag aagcggaaga gattacccgt ttgtgccacg
    14401 cgccgggcaa tgttgactac tcccgctata ttggcggtat ttattccagc gaatggttct
    14461 gggcaaaaat cctgcatgtg actcgccagg acagcgccgt ggcgcaatct gccgcatcgt
    14521 ggattgagct gtgcgactgg gtgccagctc tgctttccgg taccacccgc ccgcaggata
    14581 ttcgtcgcgg acgttgcagc gccgggcata aatctctgtg gcacgaaagc tggggcggct
    14641 tgccgccagc cagtttcttt gatgagctgg acccgatcct caatcgccat ttgccttccc
    14701 cgctgttcac tgacacctgg actgccgata ttccggtggg caccttatgc ccggaatggg
    14761 cgcagcgtct cggcctgcct gaaagcgtgg tgatttccgg cggcgcgttt gactgccata
    14821 tgggcgcagt tggcgcaggc gcacagccta acgcactggt aaaagttatc ggtacttcca
    14881 cctgcgacat tctgattgcc gacaaacaga gcgttggcga gcgggcagtt aaaggtattt
    14941 gcggtcaggt tgatggcagc gtggtgcctg gatttatcgg tctggaagca ggccaatcgg
    15001 cgtttggtga tatctacgcc tggttcggtc gcgtactcag ctggccgctg gaacagcttg
    15061 ccgcccagca tccggaactg aaagcgcaaa tcaacgccag ccagaaacaa ctgcttccgg
    15121 cgctgaccga agcatgggcc aaaaatccgt ctctggatca cctgccggtg gtgctcgact
    15181 ggtttaacgg tcgtcgctcg ccaaacgcta accaacgcct gaaaggggtg attaccgatc
    15241 ttaacctcgc taccgacgct ccgctgctgt tcggtggttt gattgctgcc accgcctttg
    15301 gcgcacgcgc aatcatggag tgctttaccg atcaggggat cgccgtcaat aacgtgatgg
    15361 cgctgggcgg catcgcgcgg aaaaaccagg tcattatgca ggcctgctgc gacgtgctga
    15421 atcgcccgct gcaaattgtt gcctctgacc agtgctgtgc gctcggtgcg gcgatttttg
    15481 ctgccgtcgc cgcgaaagtg cacgcagaca tcccatcagc ccagcaaaaa atggccagtg
    15541 cggtagagaa aaccctgcaa ccgcgcagcg aacaggcaca acgctttgaa cagctttatc
    15601 gccgctatca gcaatgggcg atgagcgccg aacaacacta tcttccaact tccgccccgg
    15661 cacaggctgc ccaggccgtt gcgactctat aaggacacga taatgacgat ttttgataat
    15721 tatgaagtgt ggtttgtcat tggcagccag catctgtatg gcccggaaac cctgcgtcag
    15781 gtcacccaac atgccgatca cgttgttaat gcgctgaata cggaagcgaa actgccctgc
    15841 aaactggtgt tgaaaccgct gggcaccacg ccggatgaaa tcaccgctat ttgccgcgac
    15901 gcgaattacg acgatcgttg cgctggtctg gtggtgtggc tgcacacctt ctccccggcc
    15961 aaaatgtgga tcaacggcct gaccatgctc aacaaaccgt tgctgcaatt ccacacccag
    16021 ttcaacgcgg cgctgccgtg ggacagtatc gatatggact ttatgaacct gaaccagact
    16081 gcacatggcg gtcgcgagtt cggcttcatt ggcgcgcgta tgcgtcagca acatgccgtc
    16141 gttaccggtc actggcagga taaacaagcc catgagcgta tcggctcctg gatgcgtcag
    16201 gcggtttcta aacaggatac ccgtcatctg aaagtctgcc gttttggcga taacatgcgt
    16261 gaagtggcgg tcaccgatgg tgataaagtt gccgcacaga tcaagttcgg tttctccgtc
    16321 aatacctggg cggttggcga tctggtgcag gtggtgaact ccatcagcga cggcgatgtt
    16381 aacgcgctgg tcgatgagta cgaaagctgc tacaccatga cgcctgcaac acaaatccac
    16441 ggcgaaaaac gacagaacgt gctggaagcg gcgcgtattg agctggggat gaagcgtttc
    16501 ctggaacaag gtggcttcca cgcgttcacc accacctttg aagatttgca cggtctgaaa
    16561 cagcttccag gtctggccgt acagcgtctg atgcagcagg gttacggctt tgcgggcgaa
    16621 ggcgactgga aaaccgccgc cctgcttcgc atcatgaagg tgatgtcaac cggtctgcag
    16681 ggcggcacct cctttatgga ggactacacc tatcacttcg agaaaggtaa tgacctggtg
    16741 ctcggctccc atatgctgga agtctgcccg tcgattgccg tagaagagaa accgatcctc
    16801 gacgttcagc atctcggtat tggtggtaag gacgatcctg cccgactgat cttcaatacc
    16861 caaaccggtc cagcgattgt cgccagcctg attgatctcg gcgatcgtta ccgtctgctg
    16921 gttaactgta tcgacacggt gaaaacaccg cactccctgc cgaaactgcc ggtggcgaat
    16981 gcgctgtgga aagcgcaacc ggatctgcca actgcttccg aagcgtggat cctcgctggt
    17041 ggcgcgcacc ataccgtctt cagccatgcg ctgaacctca acgatatgcg ccagttcgcc
    17101 gagatgcacg acattgaaat cacggtgatt gataacgaca cccgcctgcc agcgtttaaa
    17161 gacgcactgc gctggaacga agtgtattac ggatttcgtc gctaagtagc cgcatcaggt
    17221 atgtaacgcc tgatgcgatg ctggcgcgtc ttatcaggcc tacacgttgc gattttgtag
    17281 gccggataag caaaccgcat ccggcattca acgcctgatg cgacgctggc gcgtcttatc
    17341 aggcctacgc gttccgattt tgtaggccgg ataagcaaag cgcatccggc attcaacgcc
    17401 tgatgcgacg ctggcgcgtc ttatcaggcc tacacgctgc gattttgtag gccggataag
    17461 caaagcgcat ccggcacgaa ggagtcaaca tgttagaaga tctcaaacgc caggtattag
    17521 aagccaacct ggcgctgcca aaacacaacc tggtcacgct cacatggggc aacgtcagcg
    17581 ccgttgatcg cgagcgcggc gtctttgtga tcaaaccttc cggcgtcgat tacagcgtca
    17641 tgaccgctga cgatatggtc gtggttagca tcgaaaccgg tgaagtggtt gaaggtacga
    17701 aaaagccctc ctccgacacg ccaactcacc ggctgctcta tcaggcattc ccctccattg
    17761 gcggcattgt gcatacgcac tcgcgccacg ccaccatctg ggcgcaggcg ggtcagtcga
    17821 ttccagcaac cggcaccacc cacgccgact atttctacgg caccattccc tgcacccgca
    17881 aaatgaccga cgcagaaatc aacggcgaat atgagtggga aaccggtaac gtcatcgtag
    17941 aaacctttga aaaacagggt atcgatgcag cgcaaatgcc cggcgttctg gtccattccc
    18001 acggcccgtt tgcatggggc aaaaatgccg aagatgcggt gcataacgcc atcgtgctgg
    18061 aagaggtcgc ttatatgggg atattctgcc gtcagttagc gccgcagtta ccggatatgc
    18121 agcaaacgct gctggataaa cactatctgc gtaagcatgg cgcgaaggca tattacgggc
    18181 agtaatgact gtataaaacc acagccaatc aaacgaaacc aggctatact caagcctggt
    18241 tttttgatgg attttcagcg tggcgcaggc aggttttatc ttaacccgac actggcggga
    18301 caccccgcaa gggacagaag tctccttctg gctggcgacg gacaacgggc cgttgcaggt
    18361 tacgcttgca ccgcaagagt ccgtggcgtt tattcccgcc gatcaggttc cccgcgctca
    18421 gcatattttg cagggtgaac aaggctttcg cctgacaccg ctggcgttaa aggattttca
    18481 ccgccagccg gtgtatggcc tttactgtcg cgcccatcgc caattgatga attacgaaaa
    18541 gcgcctgcgt gaaggtggcg ttaccgtcta cgaggccgat gtgcgtccgc cagaacgcta
    18601 tctgatggag cggtttatca cctcaccggt gtgggtcgag ggtgatatgc acaatggcac
    18661 tatcgttaat gcccgtctga aaccgcatcc cgactatcgt ccgccgctca agtgggtttc
    18721 tatagatatt gaaaccaccc gccacggtga gctgtactgc atcggcctgg aaggctgcgg
    18781 gcagcgcatc gtttatatgc tggggccgga gaatggcgac gcctcctcgc ttgatttcga
    18841 actggaatac gtcgccagcc gcccgcagtt gctggaaaaa ctcaacgcct ggtttgccaa
    18901 ctacgatcct gatgtgatca tcggttggaa cgtggtgcag ttcgatctgc gaatgctgca
    18961 aaaacatgcc gagcgttacc gtcttccgct gcgtcttggg cgcgataata gcgagctgga
    19021 gtggcgcgag cacggcttta aaaacggcgt cttttttgcc caggctaaag gtcggctaat
    19081 tatcgacggt atcgaggcgc tgaaatccgc gttctggaat ttctcttcat tctcgctgga
    19141 aactgtcgct caggagctat taggcgaagg aaaatctatc gataacccgt gggatcgaat
    19201 ggacgaaatt gaccgccgtt tcgccgaaga taaacctgcg ctggcaactt ataacctgaa
    19261 agattgcgag ctggtgacgc agatcttcca caaaactgaa atcatgccat ttttactcga
    19321 acgagcaacg gtgaacggcc tgccggtgga tcggcacggc ggttcggtgg cagcgtttgg
    19381 tcatctctat tttccccgta tgcatcgcgc tggttatgtc gcgcctaatc tcggcgaagt
    19441 gccgccacac gccagccctg gcggctacgt gatggattca cggccagggc tttatgattc
    19501 agtgctggtg ctggactaca aaagcctgta cccgtcgatc atccgcacct ttctgattga
    19561 tcccgtcggg ctggtggaag gcatagcgca gcctgatcca gagcacagca ccgaaggttt
    19621 tctcgatgcc tggttctcgc gggagaaaca ttgcctgccg gagattgtga ctaatatctg
    19681 gcacgggcgc gatgaagcca aacgtcaggg taataagccg ctttcgcagg cgctgaaaat
    19741 catcatgaat gccttttatg gcgtgctcgg caccaccgcc tgccgcttct tcgatccgcg
    19801 cctggcatcg tcgatcacca tgcgtggtca tcagatcatg cggcaaacca aagcgttgat
    19861 tgaagcacag ggctacgacg ttatctacgg cgataccgac tcgacttttg tctggctgaa
    19921 aggcgcacat gcggaagaag aagcggcgaa aatcggtagt gcactggtgc agcacgttaa
    19981 cgcctggtgg gcggaaacgt tgcaacagca acggctgacc agtgcgcttg aactggagta
    20041 tgaaacccat ttctgccgtt ttctgatgcc aaccattcgc ggagccgata ccggcagtaa
    20101 aaagcgttat gccgggctga ttcaggaggg cgacaagcag cggatggtgt ttaaagggct
    20161 ggaaaccgtg cgcaccgact ggacgccgct ggcccagcag tttcagcagg agctgtacct
    20221 gcgcatcttc cgcaacgagc catatcagga atacgtacgc gaaaccatcg acaaactgat
    20281 ggcgggtgaa ctggatgcgc gactggttta ccgtaaacgc cttcgccgtc cgctgagcga
    20341 gtatcagcgt aatgtgccgc ctcatgtacg cgccgctcgc cttgccgatg aagaaaacca
    20401 aaagcgaggt cgccccttgc aataccagaa ccgcggcacc attaagtacg tatggaccac
    20461 caacggcccg gagccgctgg actaccaacg ttcaccactg gattacgaac actatctgac
    20521 ccgccagcta caacccgtgg cggagggaat acttcctttt attgaggata attttgctac
    20581 acttatgacc gggcaacttg ggctattttg agcaaaaaaa agagttcgcc agataccatt
    20641 ttgatgcgtg acgaatgctt tgccatccag taccatagcg ccctttccat tcctggacct
    20701 gaataacacc actacctcat aagcacggta gcgggtggtt attgcctgca attaaagata
    20761 tagagccgaa cacatatgcc ttttacactt ggtcaacgct ggatcagcga tacagaaagc
    20821 gaattgggac ttggaaccgt tgtcgcggtg gatgcgcgaa ctgtcacttt acttttccca
    20881 tctactggtg aaaaccgtct gtacgcacgc agtgattccc ccgtgacccg cgtgatgttc
    20941 aaccctggtg ataccattac cagccatgac ggctggcaga tgcaagtcga agaagtaaaa
    21001 gaagaaaatg gcttgctgac ctatatcggt actcgcctgg atactgaaga gtcctgcgta
    21061 gccctgcgtg aagttttcct tgatagcaaa ctggtgttca gcaaaccgca ggaccgtctg
    21121 tttgccgggc agattgaccg tatggaccgc tttgcgctgc gttatcgcgc gcgtaaatat
    21181 tccagcgaac agttccgtat gccttacagc ggcctgcgcg gtcagcgtac cagcctgatc
    21241 ccgcaccagc tcaacatcgc tcatgatgtt ggccgccgcc acgcgccgcg cgtcctgctg
    21301 gctgacgaag tgggtttagg gaaaaccatt gaagccggga tgatcctgca tcagcaactg
    21361 ctctctggcg ctgctgaacg tgtgctgatt atcgtcccgg aaaccttaca gcatcagtgg
    21421 ctggtagaaa tgctgcgccg tttcaacctg cgttttgcgc tgtttgatga tgagcgttat
    21481 gccgaagctc agcacgatgc ttacaacccg ttcgacaccg aacagctggt gatttgctcg
    21541 ctggattttg cccgtcgtag caaacagcgc ctggaacatc tctgtgaagc cgaatgggac
    21601 ctgctggtgg tcgatgaagc gcatcacctg gtgtggagcg aagacgcgcc gagccgcgaa
    21661 tatcaggcta ttgaacaact ggcagagcac gtgccgggcg ttctgctgct gaccgcaacc
    21721 ccggaacagt tgggaatgga aagccacttc gcccgtctgc gtctgctgga cccgaaccgt
    21781 ttccacgatt ttgcgcagtt cgttgaagag cagaaaaatt atcgtccggt tgcggacgcc
    21841 gttgccatgc tgctggcagg taacaaactg agcaatgacg aactgaacat gcttggcgag
    21901 atgatcggcg agcaggatat cgagccgctg ttgcaagcag caaacagcga cagcgaagat
    21961 gcccagagcg cccgtcagga actggtttcg atgctgatgg atcgccacgg caccagccgc
    22021 gtgctgttcc gtaacacccg taacggtgtg aaaggattcc cgaaacgcga gctgcacacc
    22081 attaaactgc cgctaccgac gcagtatcag acggctatta aagtctccgg cattatgggc
    22141 gcacgtaaaa gtgcggaaga tcgtgctcgc gatatgctct acccggagcg tatttatcag
    22201 gaatttgaag gtgataacgc cacctggtgg aacttcgatc cgcgcgttga gtggctgatg
    22261 ggctacctga ccagccatcg ctctcagaaa gtgctggtga tctgcgccaa agctgccact
    22321 gcgctgcaac tggagcaggt actgcgcgaa cgtgaaggta ttcgcgctgc ggtgttccac
    22381 gaaggtatgt cgattatcga acgtgaccgc gctgccgcct ggtttgccga agaagacacc
    22441 ggcgcacagg tactgctgtg ctcagaaatc ggttctgaag gacgtaactt ccagttcgcc
    22501 agccacatgg taatgtttga tctgccattc aacccggatc tgctggagca gcgtattggt
    22561 cgtctggatc gtatcggtca ggcgcacgat attcagatcc atgtgcctta tctggagaaa
    22621 accgctcagt cggtgctggt gcgctggtat cacgaaggtc tggatgcatt cgagcacacc
    22681 tgcccgaccg gacgcactat ttacgatagc gtatacaacg atctgattaa ctatctggct
    22741 tcaccggatc aaaccgaagg ctttgacgat ctgatcaaaa actgccgcga gcaacatgaa
    22801 gcgctgaaag cacaactgga acagggtcgt gaccgcctgc tggaaatcca ctccaacggt
    22861 ggcgaaaaag cccaggcact ggcagaaagc attgaagagc aggatgacga taccaacctg
    22921 atcgccttcg ccatgaacct gttcgatatt atcggtatca atcaggacga tcgcggcgac
    22981 aacatgatcg tgctgacgcc gtccgatcat atgctggtgc cggacttccc tggcctgtcg
    23041 gaagatggca tcaccatcac ctttgatcgt gaagtggcac tggcgcgtga agatgcacag
    23101 tttattacct gggagcatcc gctgatccgc aacggtctgg atctgatcct ttctggcgat
    23161 accggtagca gcacgatttc actgttaaaa aacaaagcgt tgccggtagg tacgctgttg
    23221 gtggaactga tttatgtggt tgaagcccag gctccgaagc agttgcagct caaccgcttc
    23281 ctgccaccga cgccggtacg tatgctgctg gataaaaacg gcaacaacct ggcggcgcag
    23341 gtagagtttg aaacctttaa ccgccagctt aacgcggtta accgtcacac cggcagcaaa
    23401 ctggttaacg ccgtgcagca ggatgttcac gctatccttc aactgggtga agcgcagatc
    23461 gagaaatctg cccgtgcatt gattgatgca gcgcgtaacg aagccgacga aaaactgtct
    23521 gccgagctgt ctcgtctgga agctctgcgt gcagtgaacc cgaacattcg tgacgacgaa
    23581 ctgaccgcca ttgagagcaa ccgtcagcag gtaatggaaa gcctggatca ggcaggttgg
    23641 cgtctggatg ccctgcgttt gatcgttgta acgcatcagt aacggagccg aaaatgggga
    23701 tggaaaacta caatccaccg caggaaccct ggttggttat cctgtatcag gatgaccata
    23761 ttatggtggt caacaagccg agcggtttgt tgtcggtgcc gggtcgtctg gaagagcaca
    23821 aagacagcgt gatgacgcgc attcagcgtg attatccgca ggcagaatcg gtgcatcgtc
    23881 tggatatggc taccagcggc gtgattgtag tggcgctgac caaagccgcg gagcgggagt
    23941 taaaacgcca gttccgcgag cgcgagccga aaaagcagta tgtagcccgc gtctgggggc
    24001 atccatcccc tgcggaaggt ctggtggatc tgccgctgat ttgcgactgg ccaaaccgcc
    24061 cgaaacagaa agtctgttac gaaacgggta aacctgcgca gacggaatat gaagtggtgg
    24121 agtatgcggc ggataacacg gcaagagtgg tgttaaaacc gattaccggg cgttcgcatc
    24181 agctgcgtgt gcatatgctg gcgctgggtc atccgattct cggcgatcgt ttttatgcat
    24241 caccagaagc gagagcgatg gcaccgcgtt tgttgctaca tgcagagatg ttgacgatta
    24301 cccatccggc gtatggcaat agtatgacgt ttaaagcgcc agcggatttt taatcgtcac
    24361 gctggcgcag ttcggtaggc ggataaggcg ttcacgccgc atccggcaat tgtgcgccga
    24421 ttgcctgatg cgacgcgggc cgcgtcttat caggcctaca gtttaaaact tatttaaacc
    24481 ctttctgctg ctttatcagc tcatatgcct gctgaatttc ctgcgctttc tgcttcgcca
    24541 tctccatcat ctcaggcggc aaacctttcg ccaccagctt atcgggatgg tgttcactca
    24601 tcagcttacg gtaggcacgt ttgatggtgg tcgcatcatc cgtcggcttc acgcccagca
    24661 cattacaggc atcttccagc gttgggccac gctgcgcttg ctgccagtta ccaccgccag
    24721 tttgctgctg ataaccgccg ccaaactgtg caccgccctg catcatgcgc aaaaactggt
    24781 caaactgagc gcgggagatc cctaattctt ctgcaatgac atacagcacc gcccgttcat
    24841 tcgggtgcag tgaaccatca gcaaacgccg cctgaatctg gatctccaga aacatacgaa
    24901 ttaagtcaaa acgaccaaag cagacactgc gaaactggcg catcttttcg cgcagcgggt
    24961 aattgtctga ttttcccacc cggaacgcat tttgcgccgc agtacgggaa gcgccatgaa
    25021 ggttcattcg gtccatcaac tggctggcga tatgaatatc agcctccgtg acgcgacctt
    25081 tggatttggt taaatgcccc atcacttcaa aagtggtggc aaaaaacagc gcctgacgct
    25141 cacgctggtt ggcgaaccac gccattttac ggctacgggc tttatcaaac atatggccta
    25201 ttaacaggcc taacactacg ccccaaaagc cgccgcccat cagtaaggcc acggccacgc
    25261 caatgatttt tccccaatac tgcatatatt ccccaaatcg acacacggat atcagggcta
    25321 tctcccacaa tataaaggtg cttttaccgt tttccggctg cggtcaattg tgggacatcg
    25381 cctataattt gcattatcat acctgtcatt caatgccgtg cctaacacca ctgacgctat
    25441 tcggacagga ttaacactag cgtagagatg acgagtacgt tagtctctga ccgtttgtca
    25501 cgcgcaacgt taccgatgat ggaacaataa aatcaacgta tgaaaaaacg tatccccact
    25561 ctcctggcca ccatgattgc caccgccctt tatagtcaac agggactggc agccgacctc
    25621 gcctcacagt gcatgttggg cgtgccaagc tatgaccgtc ctctggtaca gggcgatacc
    25681 aatgacttac ccgtgactat caatgctgac cacgcgaaag gggactaccc ggatgacgcc
    25741 gtgtttactg gcagcgtgga tatcatgcag ggtaacagcc gtctgcaggc cgacgaagtg
    25801 cagctccatc aaaaagaggc accaggacaa ccggagccgg tacgtaccgt tgatgcgctc
    25861 ggtaatgtcc attacgacga taaccaggtg atcctcaaag ggccgaaagg ctgggcgaat
    25921 ctgaacacca aagataccaa cgtctgggaa ggtgattacc agatggtggg tcgccagggt
    25981 cgcggtaaag cggacctgat gaaacaacgt ggcgaaaacc gctataccat tctggataac
    26041 ggtagcttta cctcctgtct gccgggttct gacacctgga gcgtggtagg tagcgaaatt
    26101 attcatgacc gcgaagaaca agttgcggag atctggaacg cccgctttaa ggtgggtccg
    26161 gtaccgatct tttatagccc ctatttgcag ttgccggtgg gtgacaaacg tcgctctggt
    26221 ttcttgatcc cgaacgccaa gtacaccacc accaactact ttgagttcta cctgccatat
    26281 tactggaaca tcgcgccaaa tatggatgcc accatcacgc cgcattatat gcatcgtcgt
    26341 ggcaacatca tgtgggagaa cgaattccgc tacctctccc aggcgggcgc tggcttgatg
    26401 gaactggact atctgccttc agataaagtc tatgaagatg aacacccgaa cgatgacagt
    26461 tcacgtcgtt ggttattcta ctggaaccac tccggggtca tggatcaggt gtggcgtttc
    26521 aacgtcgact acaccaaggt cagcgatcct agctacttca atgatttcga taacaagtac
    26581 ggttccagta ctgacggcta cgcaacgcaa aaattcagcg ttggctatgc ggtgcaaaac
    26641 ttcaatgcca ccgtttcaac caagcagttc caggttttca gcgaacagaa caccagtagc
    26701 tactcggcag agccgcagtt agacgttaat tactaccaga atgatgttgg tccgtttgat
    26761 acgcgtattt acggccaggc agtgcacttt gttaacacca gagacgacat gcctgaagca
    26821 acccgtgttc acctggaacc gaccatcaat ttgccgctct ctaataactg gggcagcatc
    26881 aataccgaag cgaagttgct ggcaacccat tatcagcaaa ccaatcttga ctggtataac
    26941 tccagaaaca cgaccaagct ggacgaatcc gttaaccgcg taatgccgca attcaaagtt
    27001 gacggcaaaa tggtctttga acgcgatatg gaaatgctgg ctccgggtta tacccaaacg
    27061 ctggaaccgc gcgcgcagta tttgtacgtg ccgtatcgcg atcagagcga catctataac
    27121 tacgactcgt ctctgctgca atctgactac tctggcctgt tccgggaccg gacttacggc
    27181 ggtcttgacc gtattgcctc cgctaaccag gtgacgaccg gtgtcacatc tcgcatatat
    27241 gatgatgctg ccgttgaacg ttttaatatt tccgttggtc aaatctacta tttcacggag
    27301 tctcgcactg gcgatgacaa cataacatgg gagaatgacg acaaaacggg ttcactggtg
    27361 tgggcaggcg atacttactg gcgtatctcc gagcgttggg gattgcgtgg cgggattcag
    27421 tacgatacac gtctggataa cgtagcgacc agtaactcca gcattgaata ccgtcgggat
    27481 gaagaccgtc tggtacagct gaattaccgt tacgccagcc cggaatatat tcaggctacg
    27541 ctgcctaagt actattccac tgctgagcaa tataagaatg gtatttcgca ggtaggtgct
    27601 gtcgccagct ggccaattgc cgatcgttgg tccattgttg gggcctacta ctacgacacc
    27661 aatgctaaca agcaagccga ctctatgtta ggtgtgcaat acagctcctg ctgctatgca
    27721 attcgcgtcg gttacgagcg gaagctgaac ggttgggata acgataaaca acatgcggta
    27781 tatgacaacg caatcggctt taacatcgaa cttcgcggcc tgagctccaa ctacggtctg
    27841 ggtacgcaag agatgctgcg ttcgaacatt ctgccgtatc aaaacacttt gtgatttgtt
    27901 gatttaccac gtaatccgca gtgcggttaa ttgaaatgga aaaagtatga agaactggaa
    27961 aacgctgctt ctcggtatcg ccatgatcgc gaataccagt ttcgctgccc cccaggtagt
    28021 cgataaagtc gcagccgtcg tcaataacgg cgtcgtgctg gaaagcgacg ttgatggatt
    28081 aatgcagtcg gtaaaactga acgctgctca ggcaaggcag caacttcctg atgacgcgac
    28141 gctgcgccac caaatcatgg aacgtttgat catggatcaa atcatcctgc agatggggca
    28201 gaaaatggga gtgaaaatct ccgatgagca gctggatcag gcgattgcta acatcgcgaa
    28261 acagaacaac atgacgctgg atcagatgcg cagccgtctg gcttacgatg gactgaacta
    28321 caacacctat cgtaaccaga tccgcaaaga gatgattatc tctgaagtgc gtaacaacga
    28381 ggtgcgtcgt cgcatcacca tcctgccgca ggaagtcgaa tccctggcgc agcaggtggg
    28441 taaccaaaac gacgccagca ctgagctgaa cctgagccac atcctgatcc cgctgccgga
    28501 aaacccgacc tctgatcagg tgaacgaagc ggaaagccag gcgcgcgcca ttgtcgatca
    28561 ggcgcgtaac ggcgctgatt tcggtaagct ggcgattgct cattctgccg accagcaggc
    28621 gctgaacggc ggccagatgg gctggggccg tattcaggag ttgcccggga tcttcgccca
    28681 ggcattaagc accgcgaaga aaggcgacat tgttggcccg attcgttccg gcgttggctt
    28741 ccatattctg aaagttaacg acctgcgcgg cgaaagcaaa aatatctcgg tgaccgaagt
    28801 tcatgctcgc catattctgc tgaaaccgtc gccgatcatg actgacgaac aggcccgtgt
    28861 gaaactggaa cagattgctg ctgatatcaa gagtggtaaa acgacttttg ctgccgcagc
    28921 gaaagagttc tctcaggatc caggctctgc taaccagggc ggcgatctcg gctgggctac
    28981 accagatatt ttcgatccgg ccttccgtga cgccctgact cgcctgaaca aaggtcaaat
    29041 gagtgcaccg gttcactctt cattcggctg gcatttaatc gaactgctgg atacccgtaa
    29101 tgtcgataaa accgacgctg cgcagaaaga tcgtgcatac cgcatgctga tgaaccgtaa
    29161 gttctcggaa gaagcagcaa gctggatgca ggaacaacgt gccagcgcct acgttaaaat
    29221 cctgagcaac taatggttaa aacccaacgt gttgtgatca ctcccggcga gcccgccggg
    29281 attggcccgg acttagttgt ccagcttgca cagcgtgagt ggccggtcga actggttgtt
    29341 tgtgccgatg ccactctcct taccgaccgg gcagcgatgc tcggtttgcc gctcaccctc
    29401 cgcccttatt cccccaactc ccctgcacaa ccgcaaacta cgggcacatt aacgctactt
    29461 cctgtcgcgc tacgtgaatc tgtcactgcg gggcagttag cgattgaaaa tggacattac
    29521 gtggtggaga cgctggcgcg agcgtgtgat ggctgtctga acggtgaatt tgctgcgctg
    29581 atcacaggcc ccgtgcataa aggcgtcatt aacgacgcag gcattccgtt taccggtcat
    29641 accgagtttt tcgaagagcg ttcgcaggcg aaaaaagtgg tgatgatgct ggcgaccgaa
    29701 gaacttcgcg tggcgctggc aacgacgcat ttaccgctgc gcgatatcgc agatgctatc
    29761 acccctgcac ttttgcacga agtgattgct attttgcatc acgatttgcg tacaaaattt
    29821 ggtattgccg aaccgcgcat tctggtctgc gggctgaatc cgcacgcggg cgaaggcggt
    29881 catatgggta cggaagagat agacaccatt attccggtgc tcgacgagct gcgggcgcag
    29941 gggatgaaac tcaacgggcc gctgcctgcc gataccctgt ttcagccgaa atatctcgat
    30001 aacgccgacg ccgtgctggc gatgtaccac gatcagggtc ttcccgtgct aaaataccag
    30061 ggcttcgggc gcggtgtgaa cattacgctg ggcctgccct ttattcgcac atcagtggac
    30121 cacggcaccg cgcttgaact ggcgggacgt ggcgaagccg atgtcggcag ttttattacg
    30181 gcgcttaatc tcgccatcaa aatgattgtt aacacccaat gaataatcga gtccaccagg
    30241 gccacttagc ccgtaaacgc ttcgggcaaa actttctcaa cgatcagttc gtgatcgaca
    30301 gcattgtttc tgccattaac ccgcaaaagg gccaggcaat ggtcgaaatc ggccccggtc
    30361 tggcggcatt gaccgagccg gtcggcgaac gtctggacca gctgacggtc atcgaacttg
    30421 accgcgatct ggcggcacgt ctgcaaacgc atccattctt aggcccgaaa ctgacgattt
    30481 atcagcagga tgcgatgacc tttaactttg gcgaactggc cgagaaaatg ggtcagccgc
    30541 tgcgtgtttt cggcaacctg ccttataaca tctccacgcc gttgatgttc catctgttta
    30601 gctatactga tgccattgcc gacatgcact ttatgttgca aaaagaggtg gtgaatcgtc
    30661 tggttgcagg accgaacagc aaagcgtatg gtcgattaag cgtcatggcg caatactatt
    30721 gcaacgtgat cccagtgctg gaagtaccgc cgtcagcctt tacaccacca cccaaagtgg
    30781 attccgccgt cgtgcgcctg gttcctcatg caacgatgcc tcacccggtt aaagatgttc
    30841 gtgtgttgag ccgcatcacc accgaagcct ttaaccagcg tcgtaaaacc atccgtaaca
    30901 gcctcggcaa cctgtttagc gtcgaggtgt taacgggaat ggggatcgac ccggcgatgc
    30961 gagcggaaaa tatctctgtc gcgcaatatt gccagatggc gaactatctg gcggagaacg
    31021 cgcctttgca ggagagttaa cgatgatcaa ttcgccccga gtgtgtattc aggttcaaag
    31081 cgtctacatt gaggcacaat cttcacctga taatgaacgt tacgtttttg cttataccgt
    31141 aaccatacgc aatctggggc gagcgccagt gcagttgttg gggcgttact ggctgatcac
    31201 caatggcaat ggccgtgaaa ccgaagtcca gggcgaagga gtggttggcg tccagccact
    31261 tatcgcgcct ggcgaagagt accagtacac cagcggtgca atcattgaaa ccccgctggg
    31321 caccatgcag ggtcactacg aaatgatcga tgaaaatggc gtccctttca gcatcgacat
    31381 tcccgtattc cgactcgccg ttcccacact cattcattaa aagaatatgg cgacatacct
    31441 tattggcgac gttcatggtt gttacgatga actgatcgca ttgctgcata aagtagaatt
    31501 tacccctggg aaagataccc tctggctaac gggcgatctg gtcgcgcgcg gcccgggttc
    31561 gctggatgtt ctgcgctatg tgaaatcctt aggcgacagc gtacgtctgg tgctgggcaa
    31621 tcacgatctg catctgctgg cggtatttgc cgggatcagc cgcaataaac cgaaagatcg
    31681 cctgacaccg ctgctggaag cgccggatgc cgacgagctg cttaactggc tgcgtcgcca
    31741 gcctttgctg caaatcgacg aagagaaaaa gttggtgatg gcccacgcag ggatcacgcc
    31801 gcagtgggat ctacagaccg ccaaagagtg cgcgcgcgat gtagaagcgg tgctatcgag
    31861 tgactcctat cccttctttc tcgacgctat gtacggcgat atgccaaata actggtcacc
    31921 ggaattgcgg gggctgggaa gactgcgttt tatcaccaac gcttttaccc gtatgcgttt
    31981 ttgcttcccg aacggtcaac tggatatgta cagcaaagaa tcgccggaag aggcccctgc
    32041 cccactgaaa ccgtggtttg cgattcctgg ccctgtcgct gaagaataca gcatcgcctt
    32101 tggtcactgg gcatcgctgg agggcaaagg tacgccggaa ggtatatacg cgctggatac
    32161 cggctgctgc tggggtggta cattaacctg cctgcgctgg gaagataaac agtattttgt
    32221 ccagccgtcg aaccggcata aggatttggg cgaagcggcg gcgtcttaaa cacagcctga
    32281 tataggaagg ccggataaga cgcgaccggc gtcgcatccg gcgctagccg taaattctat
    32341 acaaaattac cgccgctcca gaatctcaaa gcaatagctg tgagagttct gcgcatcagc
    32401 atcgtggaat tcgctgaata ccgattccca gtcatccggc tcgtaatccg ggaaatgggt
    32461 gtcgccttcc acttctgcgt cgatatgcgt cagatacagt ttttgcgctt ttggcaagaa
    32521 ctgttcataa acgcgaccgc cgccaatcac catgatttct ggtacgtcac cacacgccgc
    32581 gatggcttca tccaccgact tcacccacgt tacgcgatcg tccgtacccg gttgactgct
    32641 gaggataata tttttgcgtc ctggcaacgg acgaccgatt gattcccagg tatggcggcc
    32701 cataatcacg ggtttattta aggtgttgcg tttaaaccag gcgagatcgg caggcaggtt
    32761 ccacggcatg gcgttttcca tgccgataac gcgatctacc gctaacgccg caatcagact
    32821 gatcattgag atttcccgat aaaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc
    32881 gtcgactggt gcgaggatga tgttgaggaa aattttatat tctgctggcg agtccacgct
    32941 ctctccctgg actcgccgca ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg
    33001 atttacgtca ctctttatta ggatgagggt ttcgtttccg gttcatccgc catgttgccg
    33061 gtatgtttac cttcttcggt tccctgccag ccatgtcgtt gaattaatga cagatgttcg
    33121 cggtcctcgg taatgatctc acttaacatc gcgctggtgc gtttatagac cgccgcgcgg
    33181 gctttggtgt cgttctcaac cattgccatc tcttccacca tctgaatatt aaagcggcgg
    33241 aacacatcgg cacgttctcg cgcttcatac ggccccagac ctaaactttc cagtgccaga
    33301 cgcccggttt tcagcgcacc ttcgaaggtt tcacgctccg gcttttcaac gcctgcctga
    33361 cgcaaacgaa tgtagtggtc gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaaa
    33421 tgttctttca ccatctctgt cagttgcagg ttggtttgcg gatcgtcgat ggcgttaatc
    33481 agcacttccg ctttcgccgc tccggcagat tccagtaaat ccatccgcgt ggcatcgcca
    33541 taaaacactt tcataccaaa tttacgcaag gtttcgatat ggtccggatc gtgatcgagt
    33601 accaccattt tcaccccgct ggagagcagt aaacgtccgg taatctgccc aaaacgaccg
    33661 aatccggcga taatcacgcg cggctgttct tcgtcgatct catcggcttc acgcgcttcc
    33721 tcagtagaag attgctcaag gcgattgagg atcaccagca gaatcggcgt tgctgccatc
    33781 gacagcgcca ccgccagggt cagcgatttc gcccactccg gctccagcac attcgccatc
    33841 tgcgccgcgc caaataccac aaaggcaaac tcactgccct gccctaacaa caccgcaaac
    33901 caacgacgct gtttatttgg cacttgcaac ggtcgggcaa tcagccacag catggcgatt
    33961 ttgatgatga ggaaaccgag cagcaaaatg acaatgcgca atgggttttc aagcagcgtg
    34021 ccaaagtcta tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa acctttaaat
    34081 ggttcgatat cgctctccag cgcatgacgg tattccgagc ttgccagcag tacgcccgcc
    34141 agaaacgcgc ccatcgccat cgacaagccg acctcttcca gcagcaaacc aaagccaaac
    34201 acgaggaata acgccacggc actaaacact tcccgcaagc cagagcgggc tacaaaacgc
    34261 agcgccggac gcgtgacata gcgccccagc aataccacca gcaccagcgc acccgccact
    34321 tttaacgccg agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgt tgccagtagc
    34381 ggaatcatcg ccaccagcgg gatcgccgcg atatcctgga acagcagcac cgcaaaggca
    34441 ctgcgaccca tttgcgtcac catcagattg cgttcattca tcgcctgcat ggcaatcgcc
    34501 gtagaggaga gcgccagcgt catgccgatc aattccgcga cctgccagcg cagcccaagt
    34561 aacatgcaga acagccccag caggccgccg caaatcacca tctgcaatgc gccacagccg
    34621 aacactgccg cacgcagctt ccacagcctt tgtggatcga gttcgaggcc gataataaac
    34681 agcatcagca ccaccccaat ctcggcaaag tgcagaatag attcggcatc ggtcaccagt
    34741 cgcagccccc acgggccaat aatgcagccg gcgatcaggt agccaagtac cgatcccaga
    34801 ccaagacgta ccgcaatggg tacaatcagc gctgccgaac cgagataaat cagcgcctga
    34861 atcagcgtat ggctatccat gatgggcctc ctgccattcc agcagacgtt gcttatagtg
    34921 acgcgcctgc ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg
    34981 tggcagccag ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg acagcacatc
    35041 aaagcccgga tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg tcaccgccca
    35101 cagcaaatgt ttgccatgca gcgccttgcc gccgtgaccg taagcccagc cgtgcgacaa
    35161 aactttatcg atccaaagtt taagaagcgg aggaatgctg taccactgca tcggatgctg
    35221 ccagacgatc agatcggcgc gagacagcgc ctcctgctcg gcggcaatat cgatattgaa
    35281 gtcaggatag agttgataaa gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc
    35341 aagcatccgt ttattcgcat gggaatgatg cggatacgga tgcgcataaa ttataagaat
    35401 cataggtagc ccgcgtgatt acgcctttgt tttcccacaa agtgtagtca tatattcacg
    35461 cggcttatag tcaataatat tgattcagaa aagagagaaa tctgaacaat ttaccccatt
    35521 ctctgcccgc ggatcgtcat attgctggtc tgcgccagtg acatccctcg agtctccggg
    35581 gcaaacgcta cggaaatcaa caagccaaac agcgagatac ccgcccccat tagcatcgtg
    35641 ttactgatac cgtaattatt gataaagatc ggtagtgccc aggtcgaaac aatggtgcca
    35701 atacgactta aggacataat cacgcccacg gcagaggcgc ggatatctgt cgggaagagt
    35761 tcattaggat agagccactg caaattaccc ggcccgccag agaaaaaggc atacaccgca
    35821 aaggccatca ctaccagcca gatccccata tccgggatta gccccaaaac cgccagcgcc
    35881 agcgtcatca tggcaaagct gccaatcaac aatggacgcc gtccggcagt gtttaaccac
    35941 agcatcggcg gaatacagcc gagcataaag aacaggctaa tcaccacatt ccctagtgcc
    36001 gcgtttttgc caacccccaa tcccaacaaa ccaacgattt gcgggccaaa ggtgtaaatg
    36061 gcgaacattg ggatcacctg gcaggtccag atggcggcaa caaacagaac aaaaggaaaa
    36121 tggcggcgat taaacagatc gcgaaaacgg gtttgctgcg gctgctcttc atcgaaagcc
    36181 accggttcgc caaacagttt gatcatcatc tcttcgcact cttttactcg ccctttgcgt
    36241 aataaccagc ggggagattc aggcagttcg aatcgaccaa tcaaaatcaa caaacagggg
    36301 atcgccgcgc tacccagcat ccagcgccag ccgccttcca catcataaag ccagtagccg
    36361 accagatcgg cacaggtcgc gccgacatac cacatcgcgg caataaagct gatggaaaac
    36421 gcccgctgac gggtactgga gaactcggtg atcattgagg tggcgatggg ataatctgca
    36481 ccgatgacaa tgccgataag tacccgcatc accaacagtt cgacggggga tgaaacaaac
    36541 atcgtcgcca ccgatatcac gccgatggcg atgatatcaa tgaggaacat tttgcgccgt
    36601 ccgactttat cggaaatata accaaacagc gatgtgccaa cgaacagccc ggcgagcgtt
    36661 cccgcgccca gcaagccaat ccagtcagcg tccagtttca gcgccggcgt cagttgctcc
    36721 agcgccacgc caatcattac cagtacataa ccatccagaa acggaccacc gcttccccac
    36781 agcaaaatgc ggcggtgaat agaggagaat ttgagatcgt caaagtttct ggacggttgc
    36841 atggttattc ctgttttttg caatgagagt cagtgcggcg ttacgtatca aaccaacatc
    36901 agccgtaacg gaactccaca ccaaaggtgc cgcgcgggta ttcccactgt tccagcgccg
    36961 accccagccc cagaatgcga caggtgccgc actccagaca tccggcgtaa tcgaagcgca
    37021 cactgccgtc atcctgcttc ttgtacagac ctgcggggca cgctttcacc agcagctcca
    37081 gcgcctgttt atcagcatcg gccttcacaa caatgtgcgg atgctcttca tcgacattga
    37141 atttattgac gcccagtttg acgtccacat tgacgggaga agtcataaaa cggtcactcc
    37201 tttcatgcca tccttgatta gattgatgaa gcccactttc ttgccgtggc ggaggatttt
    37261 cttgcgcatc agttccggcg cgctgccatc aatggtgaac aggtcacgcg ccacacccac
    37321 cgccagctcc gggtagccgc taaacatgcg tgggttatca aggaacgccg gtagtttctg
    37381 gtacatacgc atatcgcgca gcggaccact ctcaagatgc tgacgatatt ccgccagttt
    37441 ttgcttactg aaatcgtcgc ttttcatcgc tgaaagcacg gtttttgctg cggcttcccc
    37501 ggcggcaatc gccagatcca taccgcgaat ggtaaaaccg aggttcatac acattccggc
    37561 ggcatcaccg gcaatcaata cgccgtcacc aaccaactcc ggcagcatgt tgatgcctgc
    37621 ttccggcact acgtgagcgg aatattccac cagcttgccg cccgcgatca gcggtgcaac
    37681 ggccggatgc tgtttgaaat cttccagcat ttgcggcacc gattttttcg cgtcatgcag
    37741 atgatgcaaa ccacaaacca gccccagcga cagggtgttt tcattggtat aaaggaagcc
    37801 gccgcccatc aggccatcgg tgggtgatcc cgcaaacagg caagccgccc cctgattacc
    37861 ctgcaactga aaacggtctt caataaccga cttcggtaac tcgatcagtt ccttcacgcc
    37921 aaccgccaca tccgtcggtt tgacgcgttt tgccatcccc aatttttcgg caaggatgga
    37981 gttcacccca tcagcaagga tcaccgtttt cgcttcaatc acatcgccat cggcttctac
    38041 accgacgact ttgccatcgc gctgtacgag gttatcgacg cggatcccgg taattaactg
    38101 cgcgcccgct tcttcggcct gctccatcag ccaggcatca aatttactgc gcaaaacgga
    38161 gtaagaacgc tgggatggcg aggtttcgtc accattgcag tagtccatag tcatcgctga
    38221 cttttccgtc ataaacgcga gtttttcatg ggtgatcagg cgttctacgg gggcggagtc
    38281 ggcgaaacca ggaataatgt gttccagact gtgggcatag agacgcccgc cggtgacgtt
    38341 cttggcacct gcggaattgc cacgctcgat aactaacact tgcgcacctt cgcgggcgag
    38401 caccagtgcg gcaaccgaac cggcaagccc tgcaccgacg atgatggcgt caaagatatc
    38461 ttcggacata agagctccct gtcagcggca gggccaaaat gcagccctgc cagagtggat
    38521 caacgcgcta aagctgcggt cagcgccgga aggatcttca cggcgtcgcc aacaatgccg
    38581 taatccgcgt actggaagat cggcgcattt ttatctttat tgatggcgaa aatggtttgc
    38641 gacgcgttag cgccaaccat gtgctggatc tgcccggaga tccccaccgc caagtacagt
    38701 tcaggtttca gcatcaggtt ggagataccg acatagcgtt cgtgctccat ccatttttcg
    38761 ttttccgcca ccggacgaga acaggccaac tccgcaccta tcgccttgca aagctgttct
    38821 gccagcgcaa tgttctcttt gctgccaata ccgcgaccga cgctgaccac cagacgggct
    38881 ttgtcgagat cgacgctgtt gctctggcgc gcctgggttg ccgtgcgggt aatcgccaca
    38941 gccggagcct gccactccac ggtgtgcgtt tcgccagtgc gtgacgcgtc tggctgagcc
    39001 gcatcgaacg tgccgctgct gatggtcagt accgcatacg gcgtggcaat gcgttcttcg
    39061 ccaatcgcca gaccaccgta aaccatgtgt ttcactgtcg ctttaccgtc ctgtacgctg
    39121 acggtgctgg catcgttaga caccgccgct ttaaggcgat aacccagttt tgccgccagt
    39181 aatttgccgc gacgggtgtt tggcagcagc accaggccgt ctgcgccgtg ctggcgaata
    39241 gtgtcagcca tgacaccggc gtaatcttcg atcatccgat cgtccggttt gccgtttaat
    39301 ttccagacat gattagcgcc gagctggatt gcctgtgcgc cgtcggcatc attgaggaca
    39361 aaggtgttga tttgattagc taaagcctgc gcaccgttca tcagttccgg cagacgagaa
    39421 ggggtatcgc tgaataccca gacttgagaa aacgtgttca tagcatcccc tgtaattaaa
    39481 tgactttgcg aagattttca gcaaatgcgg cgatctgttc ttcgccgtcg ccttcaatca
    39541 cgatgcgctg acgttcgcgc tgtttcggcg cggcaacctg ttgttctgac caggctgcct
    39601 ctgcgttaaa accaatatcc gccgccgacc atacctggac gggctttttc gccgcgccga
    39661 gaatggcttt catcgaagga atttgtgggg aattgatatc agtggaaaca gcaacaaccg
    39721 caggcagcgg aatgcttaag gtttcggttt catcttccag ttcgcgctca acggtgaggg
    39781 tatctgcagt cagggagata attttgctga cgccgttaac tgccggaata ttgaggattt
    39841 cgcccaccag cagaccaacc tgctgggcat aaaggtcgga agaaccatcg ccacagagga
    39901 tcaaatcaaa gcctgctttc tgggcggctg cagccagtgc gctcgccgtt tgttgcggca
    39961 gtgcctgctc gaactggtca tcaatcacca caatcagttc atccgggccg cgcgatagca
    40021 catctttacg ccctttggcg ttggtcaggg ctttaccgcc cacacttaag gccgtcacct
    40081 gcgcctctgc tgcctgttgc tttagctggc aagccgcttc aatagcgttc agatcgtatt
    40141 ggcttatttt ggcatcggct ttgctgaagt ctaatgaacc atcagcatta ttgaccgcaa
    40201 tatcctgttc atcaggcacg cacttatagc aagtaataat cttcattgca tctccagaaa
    40261 tcatgaagga aacgtcgttc atatcggtgg tgttaattac agatatcacg ccctttaatt
    40321 tggaaataaa actttaatca ccaatattga aaatgtcaca cgcagataaa tccaactttc
    40381 aatattgtta agttcctcac caatattgaa aacacggcgt agcaaaaaga aattttcaat
    40441 attgttttat ggatcaccaa tattgaaagt cagatgttat ttttttactc acaacagagc
    40501 ataacaaact gattattaat caaaattaac gaaaaaacgc cctctattca cccgaaacaa
    40561 aaatgtga
//
LOCUS       DAPHBJ010000017        39441 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.017, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000017 DAPHBJ010000000
VERSION     DAPHBJ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.017"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(105..800)
                     /gene="aqpZ"
                     /locus_tag="RHZ76_000849"
     CDS             complement(105..800)
                     /gene="aqpZ"
                     /locus_tag="RHZ76_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HDV2811541.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            1225..2883
                     /locus_tag="RHZ76_000850"
     CDS             1225..2883
                     /locus_tag="RHZ76_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HDV2811542.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRMAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2880..3836)
                     /locus_tag="RHZ76_000851"
     CDS             complement(2880..3836)
                     /locus_tag="RHZ76_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HDV2811543.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRECMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3987..5102
                     /gene="macA"
                     /locus_tag="RHZ76_000852"
     CDS             3987..5102
                     /gene="macA"
                     /locus_tag="RHZ76_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HDV2811544.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5099..7045
                     /gene="macB"
                     /locus_tag="RHZ76_000853"
     CDS             5099..7045
                     /gene="macB"
                     /locus_tag="RHZ76_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HDV2811545.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(7118..7342)
                     /gene="cspD"
                     /locus_tag="RHZ76_000854"
     CDS             complement(7118..7342)
                     /gene="cspD"
                     /locus_tag="RHZ76_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HDV2811546.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7665..7985
                     /gene="clpS"
                     /locus_tag="RHZ76_000855"
     CDS             7665..7985
                     /gene="clpS"
                     /locus_tag="RHZ76_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HDV2811547.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            8016..10292
                     /gene="clpA"
                     /locus_tag="RHZ76_000856"
     CDS             8016..10292
                     /gene="clpA"
                     /locus_tag="RHZ76_000856"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HDV2811548.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     repeat_region   10502..10706
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=10502..10529
                     /rpt_unit_seq="gttcactgccgtacaggcagcttagaaa"
     gene            complement(10750..10837)
                     /locus_tag="RHZ76_000857"
     tRNA            complement(10750..10837)
                     /locus_tag="RHZ76_000857"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10801..10803),aa:Ser,seq:gga)
     gene            complement(11091..11309)
                     /gene="infA"
                     /locus_tag="RHZ76_000858"
     CDS             complement(11091..11309)
                     /gene="infA"
                     /locus_tag="RHZ76_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDV2811549.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(11594..12298)
                     /gene="aat"
                     /locus_tag="RHZ76_000859"
     CDS             complement(11594..12298)
                     /gene="aat"
                     /locus_tag="RHZ76_000859"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HDV2811550.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(12340..14061)
                     /gene="cydC"
                     /locus_tag="RHZ76_000860"
     CDS             complement(12340..14061)
                     /gene="cydC"
                     /locus_tag="RHZ76_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HDV2811551.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(14062..15828)
                     /gene="cydD"
                     /locus_tag="RHZ76_000861"
     CDS             complement(14062..15828)
                     /gene="cydD"
                     /locus_tag="RHZ76_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HDV2811552.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15951..16916)
                     /gene="trxB"
                     /locus_tag="RHZ76_000862"
     CDS             complement(15951..16916)
                     /gene="trxB"
                     /locus_tag="RHZ76_000862"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDV2811553.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            17461..17955
                     /gene="lrp"
                     /locus_tag="RHZ76_000863"
     CDS             17461..17955
                     /gene="lrp"
                     /locus_tag="RHZ76_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HDV2811554.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            18090..22079
                     /gene="ftsK"
                     /locus_tag="RHZ76_000864"
     CDS             18090..22079
                     /gene="ftsK"
                     /locus_tag="RHZ76_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDV2811555.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYIARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPAQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVPPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQQQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPV
                     DTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLA
                     RSFSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDI
                     AGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELS
                     VYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRM
                     MRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKAR
                     AAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDM
                     LYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGA
                     EELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNR
                     EVLAPPPFD"
     gene            22234..22845
                     /gene="lolA"
                     /locus_tag="RHZ76_000865"
     CDS             22234..22845
                     /gene="lolA"
                     /locus_tag="RHZ76_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HDV2811556.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22856..24199
                     /gene="rarA"
                     /locus_tag="RHZ76_000866"
                     /gene_synonym="mgsA"
     CDS             22856..24199
                     /gene="rarA"
                     /locus_tag="RHZ76_000866"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HDV2811557.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRDAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            24290..25582
                     /gene="serS"
                     /locus_tag="RHZ76_000867"
     CDS             24290..25582
                     /gene="serS"
                     /locus_tag="RHZ76_000867"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDV2811558.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            25821..28265
                     /gene="dmsA"
                     /locus_tag="RHZ76_000868"
     CDS             25821..28265
                     /gene="dmsA"
                     /locus_tag="RHZ76_000868"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HDV2811559.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIVKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            28276..28893
                     /gene="dmsB"
                     /locus_tag="RHZ76_000869"
     CDS             28276..28893
                     /gene="dmsB"
                     /locus_tag="RHZ76_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDV2811560.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28895..29758
                     /gene="dmsC"
                     /locus_tag="RHZ76_000870"
     CDS             28895..29758
                     /gene="dmsC"
                     /locus_tag="RHZ76_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HDV2811561.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(29793..30419)
                     /locus_tag="RHZ76_000871"
     CDS             complement(29793..30419)
                     /locus_tag="RHZ76_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDV2811562.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDTPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRLSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            30733..31881
                     /locus_tag="RHZ76_000872"
     CDS             30733..31881
                     /locus_tag="RHZ76_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2811563.1"
                     /translation="MSTYTQPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            32091..33521
                     /locus_tag="RHZ76_000873"
     CDS             32091..33521
                     /locus_tag="RHZ76_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV2811564.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(33522..34430)
                     /locus_tag="RHZ76_000874"
     CDS             complement(33522..34430)
                     /locus_tag="RHZ76_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2811565.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMSLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSCIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            34530..35120
                     /locus_tag="RHZ76_000875"
     CDS             34530..35120
                     /locus_tag="RHZ76_000875"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HDV2811566.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            complement(35202..35942)
                     /gene="pflA"
                     /locus_tag="RHZ76_000876"
     CDS             complement(35202..35942)
                     /gene="pflA"
                     /locus_tag="RHZ76_000876"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HDV2811567.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(36134..38416)
                     /gene="pflB"
                     /locus_tag="RHZ76_000877"
     CDS             complement(36134..38416)
                     /gene="pflB"
                     /locus_tag="RHZ76_000877"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDV2811568.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(38471..39328)
                     /gene="focA"
                     /locus_tag="RHZ76_000878"
     CDS             complement(38471..39328)
                     /gene="focA"
                     /locus_tag="RHZ76_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HDV2811569.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         9787 a         9612 c        10443 g         9599 t
ORIGIN      
        1 caatatgtgg cttagctcat gaaaggagcg caacaagatg atgaatcaac atataacaac
       61 atcttaaaaa aaggcctgac attacgccag gccttctgcg ttaattaatc acgcttttcc
      121 agcagggtcc ggtaaatcag accaccgata atgccgccga caattggcac cacccagaag
      181 aaccacagtt gttctaatgc ccagccgccc tggaagatag caaccgcggt gctgcgcgcc
      241 gggttaacag aagtgttagt caccggaata ctgattaagt gaatcagggt taaggccaga
      301 ccaatagcga tcggcgcaaa acctgccgga gcgaatttgt cggttgcgcc gtggatcacc
      361 aacaggaaac ctgcactcaa taccagttca actaccagcg cggaaagcat ggaataaccg
      421 cctggtgaat gctcgccata accgttagaa gcaaaaccgc tggctgccgc gtcaaaaccc
      481 gttttaccac tggcaattaa atacagcagc gctgccgcaa caataccgcc gacaacctgg
      541 gcaattacgt agccaacgac ttcttttgcc ggaaaacgtc cgccagccca taaaccaata
      601 gtgaccgccg ggttaaaatg accaccagaa atatgaccaa cagcaaaggc catcgtcaga
      661 acggtcagac cgaacgccaa cgccacgccg gcaaaaccaa tgcctaattc cgggaagcct
      721 gcggccagta cagcactacc acagccacca aaaacaagcc agaaagtacc aaaacattca
      781 gctgctaatt ttctgaacat atccacctca attaaaaatt gaccctgtga aaaatatggt
      841 cgttttatag ggccgtcgta aaaagtgacg acggaaataa tgcgcggcta ttttaaaaac
      901 gaaggcgagt cattcaccag ataaataaat ccagtaaatt tgatttaggg caacagcggg
      961 ttgccccata tagtcgtttg tctgattgac agtgtagtgc aagcaaaaga tttaatcctt
     1021 taggcgtaat aaaaaataat ttatcatgct aattatttga ttttgttgtt tttgcagact
     1081 tatcagcaag agggagtata acgcgattat tcgctcattt ttcagacatt tgccatgctt
     1141 aaatgtgatg tcatcacgta ttagcaaggc ctttcccgtt atactgccag cgtaaaggat
     1201 aagtcacata tttctggagg ggatatgatt cttgagcgcg ttgaaattgt gggttttcgc
     1261 ggtatcaacc gtttgtcgtt gatgctggaa caaaacaacg tcctgattgg ggagaacgcg
     1321 tggggtaaat ccagcttgct ggacgcctta actctgctgc tatcgccaga atcagatctc
     1381 taccattttg agcgcgacga tttctggttc ccgccgggag atatcaacgg gcgagaacat
     1441 catctgcata ttattttgac cttccgcgaa tcgctgccag gccgacatcg ggttcgccgt
     1501 tatcggccgc tggaagcgtg ctggacgcca tgcaccgatg gctatcaccg tattttttat
     1561 cgtctggaag gggagagtgc ggaagacggc agcgtgatga cactgcgcag ttttctcgat
     1621 aaagacggac atccgattga tgtcgaggat attaacgatc aggcacgcca tctggtgcgt
     1681 ttaatgccgg tgctgcgctt gcgtgatgcc cgttttatgc gccgtattcg taacggcacg
     1741 gtgccaaatg tccctaatgt ggaagtcacc gcgcgccagc tcgatttcct cgcccgtgag
     1801 ttatcctcac atccgcaaaa tctctctgat gggcagattc gtcagggact ttccgcaatg
     1861 gtacagctgc ttgagcatta tttctctgag cagggggccg gacaggcgcg atatcgttta
     1921 atgcggcggc gagccagcaa tgagcaacga agctggcgct atctggatat catcaaccgg
     1981 atgattgacc gacctggtgg gcgctcgtat cgggttattt tgctcggcct gtttgctact
     2041 ttgttgcagg caaaaggcac attgcgactg gataaagacg cccgtccatt gttgctgatc
     2101 gaagatccag aaacccgttt acaccccatt atgctttcag ttgcctggca tctgttgaat
     2161 cttctgccat tgcagcgcat tgccaccacc aactcgggtg agttgctttc gttaacgccg
     2221 gtagagcatg tttgccgact ggtacgtgag tcctcacgcg ttgccgcctg gcgtctgggg
     2281 ccgagtggct tgagtaccga agatagccga cgcatatcct ttcacattcg ttttaaccgt
     2341 ccgtcatcgc tgtttgcacg ctgctggttg ctggtggaag gggaaacgga aacctgggtt
     2401 atcaatgaac tggcgcgtca gtgcggacat cattttgatg ccgaagggat caaggtcatt
     2461 gagtttgccc agtccgggct aaagccactg gttaaatttg cccgccgaat ggggattgaa
     2521 tggcatgtac tggtcgatgg cgatgaagca gggaagaaat atgccgctac ggtacgcagc
     2581 ctgttgaata acgatcggga agccgaacga gaacatttaa cggcgttacc ggcgctggat
     2641 atggaacatt ttatgtatcg ccagggattt tccgatgtgt tccaccgcat ggcgcaaatc
     2701 ccggaaaatg taccgatgaa tctacgcaaa attatctcga aagcgatcca tcgctcttcc
     2761 aaacccgatc ttgccattga agtggcaatg gaggcaggac gtcgtggtgt ggactccgta
     2821 ccgacgctgc tgaaaaaaat gttctcacgc gtgctgtggc tggcgcgcgg tcgcgcggat
     2881 taaccgcgaa acatcgtggc catttgtggc tgaatagcgt cgagcatctc atagcgccga
     2941 cggtattcag cccgtttttt actggcgatt tcggcaatct cttttcgtgc tatctgtgct
     3001 ggaaggcggt aatggcgttc agcatcacat acgccgccaa ccgattccca gaaagcgttg
     3061 taatcagcgt ggatcttgcc ttctttatcg cgataacgca ggctgcggta aatatgcgtt
     3121 tcattgctga cggcaataat ctgctctacc tgcaaacgtt gggcaaacag acaggccgct
     3181 tccatcacga ggcgtttggg aaatagcccg tggcaggctt tcgtcgcatt ctggatttcc
     3241 tgatgtggaa tttcccattt tgcgccttgc agtccgccaa taaacatcgt tcttttcccc
     3301 tgatattcac acagggtaaa cgtgatctct gccagaggaa taccttcgct gttgcggaac
     3361 aggattgtgc tgtcaccttc tttatccatt gagatcatca tggtcagctc aagcgtgaac
     3421 tgctcgccgt ttttgccttc cagcttcgcc agttgcagcc cgggggtatt caaatataag
     3481 ctgaattctt ccgccgacat acattcgcgg agtaacgcat aatggtaacg taacgcctcc
     3541 agcaattgct tacggctaag attcgccgca aggtaagggc gatgcagacg cacaggcagt
     3601 cgcggctggc gcgttaacaa tacattgaga ttaggccagt gggaaagttc gttcatccac
     3661 tcaacgctta aacgcggcat aatcaacgag cgcagcaaaa atttctggcg aaaactacgg
     3721 cgatgccaga atttacccgg ccgacactgt ccacgtgcca gactaagaaa aagtgacagg
     3781 ctgctgagag attcagatgg cgtaaaggtc cgttcagtta gctgcgacat attcatgaaa
     3841 tcaatggtta tacatgacgt cgatttcacc attgcgtatc ttaaccaaac atcaatagtg
     3901 tgattactaa cgtaaatttt agggggttgt tgatatttcg ttgaagttaa tgacccggat
     3961 tggcatatgg agtattcaga atatttatga aaaagcggaa aaccgtgaag aagcgttacg
     4021 ttattgcgct ggtgatagtc atcgccggac tgattacgtt atggagaatt cttaacgcac
     4081 ccgtgccgac ttatcagaca ctgattgtgc gccccggtga tttacagcaa agcgtgctgg
     4141 cgaccggaaa gctggacgcg ctgcgtaagg ttgacgtggg cgcgcaggtc agcggtcagt
     4201 tgaaaacgct gtcggtggcg attggcgata aagtaaaaaa agaccagctt ttaggggtca
     4261 ttgatcctga acaggctgaa aaccagatca aggaggtcga agcaacgctg atggagctac
     4321 gcgcgcagcg gcagcaggcg gaagcggagc tgaaactggc gcgggtgacg tattcccgtc
     4381 agcaacgtct ggcacaaacg caggcggttt cacagcagga tctcgacacc gccgcgacgg
     4441 agatggctgt gaaacaggcg caaattggca ccattgacgc gcaaatcaag cgcaatcagg
     4501 cttctctcga tacggctaaa accaatctcg attacactcg catcgttgcc ccgatggccg
     4561 gggaagtcac gcaaatcacc actctgcaag gccagacggt gattgccgca caacaagcac
     4621 cgaacattct gacgctggca gatatgagca ccatgctggt aaaagcgcag gtttctgaag
     4681 cggatgtaat ccacctgaag ccggggcaaa aagcctggtt tacggtactt ggcgatccac
     4741 tgacgcgcta cgaggggcaa atcaaggatg tactaccgac gccggaaaag gttaacgacg
     4801 ctattttcta ttacgcccgt tttgaagtcc ccaaccccaa tggtttgctg cggctggata
     4861 tgactgcgca agtgcatatt cagctcaccg atgtgaaaaa tgtgctgacg atccctctgt
     4921 cggcgttagg cgatccggtt ggcgataatc gttataaagt caaattgttg cgtaatggtg
     4981 aaacacgcga gcgtgaagtg acgattggcg cacgtaacga taccgatgtt gagattgtca
     5041 aagggctgga agcgggcgat gaagtggtga ttggtgaggc caaaccagga gctgcacaat
     5101 gacgcctttg ctcgaattaa aggatattcg tcgcagctat cctgccggtg atgagcaggt
     5161 tgaggtgttg aagggcatca ccctcgatat ttatgctggt gagatggtgg cgattgttgg
     5221 cgcttcgggt tccggtaaat cgaccctgat gaatattctc ggctgtctgg ataaggccac
     5281 cagcggtacc tatcgcgtcg ccggtcagga tgttgccacg ctggacgccg atgcgctggc
     5341 gcaactgcgc cgcgagcatt tcggctttat tttccagcgt taccatttgc tttcgcattt
     5401 aaccgccgag cagaacgttg aagttcccgc cgtctatgct ggtcttgagc ggaaacagcg
     5461 gctgcttcgc gcccaggagt tgctgcaacg gctggggctg gaagaccgta cagagtatta
     5521 tccggcacag ctttcgggtg gtcagcaaca gcgcgtcagc atcgcgcggg cattgatgaa
     5581 cggcggtcag gtaattcttg ccgatgaacc aaccggcgca ctggacagcc attctggcga
     5641 agaggtgatg gcgatcctgc atcagctgcg cgatcgcggg catacggtga ttatcgtcac
     5701 ccacgatccg caggtcgctg ctcaggccga gcgggtgatc gaaattcgcg acggcgaaat
     5761 tgtgcgcaat cctcccgcca ttgaaaaagt gaatgttgct ggcgggacgg agcctgttgt
     5821 caacacggtg tctggctggc ggcagtttgt cagcggtttt aacgaggcgc tgacgatggc
     5881 atggcgggcg ctggcagcga ataaaatgcg tactttactg accatgctgg ggattattat
     5941 cggtattgcg tcggtggttt ccattgtcgt ggtgggtgac gccgccaaac aaatggtgct
     6001 ggcggatatt cgttctattg gtacgaatac tattgatgtc tatcccggga aagattttgg
     6061 cgatgacgat ccgcaatatc aacaggcgct gaagtacgac gacttaatcg ccatccaaaa
     6121 acaaccgtgg gtcgcctcag ccacacctgc cgtctcgcaa aacctgcgcc tgcgttataa
     6181 caatgttgat gttgctgcca gtgccaatgg cgtgagcggc gattatttta atgtctatgg
     6241 catgaccttc agtgaaggga acacctttaa tcaggagcag ctgaacggtc gtgcgcaggt
     6301 cgtggttctc gacagtaata ctcgccgcca gcttttcccc cataaagcag atgtggttgg
     6361 cgaggtgatt ctggtcggca atatgcccgc cagagtcatt ggtgtggcgg aagaaaaaca
     6421 gtcgatgttt ggtagcagta aagtgctgcg tgtctggcta ccttacagca cgatgtccgg
     6481 gcgagttatg ggccagtcgt ggcttaactc cattactgtc agggtgaaag aaggatttga
     6541 cagcgccgag gcggaacagc aactcacgcg tttactttca ctgcgccacg gaaagaagga
     6601 tttctttacc tggaacatgg acggcgtctt gaaaactgtt gaaaagacca cacgtacttt
     6661 acaactgttt ctgacgctgg tggcggtgat ttcgctggtg gtgggcggta ttggtgtaat
     6721 gaatattatg ctggtgtcag tgaccgagcg gacgcgggaa attggcattc gcatggctgt
     6781 aggtgcgcga gcaagcgatg ttttgcaaca gttcctgatc gaagccgtac tggtttgcct
     6841 ggtcggtggc gcgttgggaa taacactgtc actgttaatt gctttcacct tgcagctttt
     6901 cttacccggc tgggagattg gtttttcacc gttggcgctg ctgctggcgt ttctctgctc
     6961 gacggtcacc gggattttat ttggctggtt acccgcacga aatgcggcac gactggatcc
     7021 agtagatgct ctggcacgag agtaattttt gagataaaaa tgccagccga tcgggctggc
     7081 attttgcctt taggatgtac acaatgagac agaagagcta tgcgactgcc gcttctactt
     7141 cgacgggcac aataacactg gcgtgattgc cttttggccc ctggtggaca tcaaactgaa
     7201 cggattgtcc agcttttagc gttctgtaac catccatctg aatggtggaa taatgagcga
     7261 aaatatcttc gccgccgcct tcagggcaga tgaaaccaaa ccctttggca ttgttgaacc
     7321 acttaacagt acccttttcc atgcttcgac atccttcgca aatcttatac aagtaagatg
     7381 gaataaaccg gggtcagaga gggggctgtt caaaacctcg ccaactctag aaatacaatt
     7441 tagagaatta gggcgagccg tcaagcattt gacaggggac agggggcagg tatgaatcaa
     7501 aaatttgaag cagttaacgc tattgacagg aatgtgacag atgtcgctga tgccaacgat
     7561 agatgatagt tatctatcat gtggagtaga ttggtcaggc aaataagctc ttgtcagcgg
     7621 cagggcgttc tgccgataac cgtaaccgaa gatgataact gacaatgggt aaaacgaacg
     7681 actggctgga ctttgatcaa ctggcggaag aaaaagttcg cgacgcgcta aaaccgccat
     7741 ctatgtataa agtgatatta gtcaatgatg attacactcc gatggagttt gttattgacg
     7801 tgttacaaaa attcttttct tatgatgtag aacgtgcaac gcaattgatg ctcgctgttc
     7861 actaccaggg gaaggccatt tgcggagtct ttaccgccga ggttgcagaa accaaagtgg
     7921 cgatggtgaa caagtacgcg agggagaatg agcatccatt gctgtgtacg ctagaaaaag
     7981 cctgaatgca ggcataaaaa ttgggggagg tgcctatgct caatcaagaa ctggaactca
     8041 gtttaaatat ggctttcgcc agagcgcgcg agcaccgtca tgagtttatg accgtcgagc
     8101 acttgttact ggcgctgctc agtaacccat ctgcccggga ggcgctggaa gcgtgttctg
     8161 tggatttggt tgcgctccgt caggaactgg aagcctttat tgaacaaacc acacccgttc
     8221 tgcctgccag tgaagaggag cgcgacacac agccgacgct gagttttcag cgtgtactgc
     8281 aacgtgcggt cttccatgtc cagtcctccg gtcgcaatga ggttaccggt gcaaacgttc
     8341 tggtcgctat ctttagcgaa caggagtcgc aggcggcata tctgttgcgt aaacatgaag
     8401 tcagccgtct cgatgtggtg aattttatct ctcatggcac gcgtaaagac gagccgacac
     8461 agtcttctga tcctggcagc cagccaaaca gcgaagaaca agctggtggg gaggaacgta
     8521 tggagaattt cacgacgaac ctgaatcagc ttgcgcgcgt gggcggaatc gacccactga
     8581 ttggtcgtga gaaggagctt gagcgtgcta ttcaggttct ctgccgtcgc cgtaaaaaca
     8641 acccgctgct ggtgggggaa tctggtgtcg gtaaaaccgc gattgcagaa ggtcttgcct
     8701 ggcgaattgt tcagggcgat gtgccggaag tgatggctga ctgtacaatt tactctctcg
     8761 atatcggttc tctgttagcg ggcactaaat atcgcggcga ctttgaaaaa cgttttaaag
     8821 cgttgctcaa gcagctggag caggacacta acagcatcct gtttattgat gagatccaca
     8881 ccattatcgg tgcgggtgca gcgtctggtg gccaggtcga tgcggctaac ctgatcaaac
     8941 cgttgctctc cagcggtaaa attcgcgtaa ttggttcgac aacctatcag gagttcagca
     9001 acattttcga gaaagaccgt gctctggcgc gtcgcttcca gaaaattgat attactgaac
     9061 cgtcgatcga agaaactgtt caaatcatca atggcctgaa accgaagtat gaagcgcacc
     9121 acgacgtgcg ttataccgca aaagcggtgc gtgcggcggt agagctggcg gtgaaataca
     9181 ttaacgatcg tcatctgccg gataaagcca ttgatgttat cgacgaagcg ggcgctcgcg
     9241 cacgtctgat gccggtaagc aaacgcaaga aaaccgttaa tgtggcggat attgagtccg
     9301 tggtggcccg tattgcacgc attccagaga agagtgtttc tcagagtgac cgcgataccc
     9361 tgaaaaacct cggcgatcgc ctgaaaatgc tggtcttcgg ccaggataaa gccattgagg
     9421 cgctgactga agccattaag atggcgcgtg caggtttagg tcacgaacat aaaccggtcg
     9481 gttcgttcct gtttgccggt cctaccgggg tcgggaaaac agaggtgacg gtacagcttt
     9541 cgaaagcgtt gggcattgag cttctgcgct ttgatatgtc cgagtatatg gaacgccata
     9601 ccgtcagccg tctgattggt gcgcctccgg gatacgttgg ttttgatcag ggcggtttgc
     9661 tgactgatgc ggtcatcaag catccacatg cggttctgct gctggacgaa atcgagaaag
     9721 cgcaccctga cgtgttcaat attctgttgc aggtgatgga caacggtacg ctgaccgata
     9781 acaacggacg caaagcggac ttccgtaacg tggtgctggt gatgaccacc aacgccgggg
     9841 tacgtgaaac tgagcgtaaa tccattggtc ttatccacca ggataacagc accgatgcga
     9901 tggaagagat taagaagatc tttacaccgg aattccgtaa ccgtctcgac aacattatct
     9961 ggtttgatca tctgtcaacc gacgtgatcc atcaggtggt ggataaattc atcgtcgagt
    10021 tgcaggttca gctggatcag aaaggtgttt ctctggaagt gagccaggaa gcgcgtaact
    10081 ggctggccga gaaaggttac gaccgggcaa tgggcgcacg tccgatggcg cgtgtcatcc
    10141 aggacaacct gaaaaaaccg ctcgccaacg aactgctgtt tggttcgctg gtggacggcg
    10201 gtcaggtcac cgtcgcgctg gataaagaga aaaatgagct gacttacgga ttccagagtg
    10261 cacaaaagca caaggcggaa gcagcgcatt aatctgattg tcaggtaggt tggtcaagtc
    10321 cgtaatctcg aaagaggttg cggacttttt atttatgggg tggaggttca gacccttttt
    10381 ttaatgatga tggtaagttg ttgataatta gtgctgcggg aaggtaagga taaaaaaggg
    10441 tgctgcagga gaatgggatg gttttgcttt attaacaacg ggctaaacgt gtagtatttg
    10501 agttcactgc cgtacaggca gcttagaaaa acctaccgtc ttggctagcg gttgcagcga
    10561 acgttcactg ccgtacaggc agcttagaaa ggaacaatct tgcaaaggct gtgaaagttg
    10621 gcgttcactg ccgtacaggc agcttagaaa ttcacaggta acatactcca cccaccatgt
    10681 tcactgccgt acagacagat aaaatgcgaa aaaaaagctc gcactttcgt acgagctctt
    10741 ctttaaatat ggcggtgagg gggggattcg aacccccgat acgttgccgt atacacactt
    10801 tccaggcgtg ctccttcagc cactcggaca cctcaccaaa ttgttttgct gccaaacctc
    10861 atgggtggca acggggcgct actataggga gttggagtaa aacggtcaag aagaatttta
    10921 atgataatta ttgtttgctc atactgtaaa caacttgtgc agtatatcta catcgagaca
    10981 agttatggac ttatacttcc aaagtacttc atacatatca caaaataaaa aggccggtta
    11041 aaccgacctt ttactcgttc tttctcttcg cccatcaggc ggtaaaacaa tcagcgacta
    11101 cggaagacaa tgcggccttt gctcaggtcg tacggggtca gttcaacagt cactttgtcg
    11161 cccgtcagga tgcggatgta gtttttgcgc attttaccgg agatgtgtgc agtaaccacg
    11221 tgaccgtttt ctaactctac gcggaacatg gtattaggca acgtttcaag aacggtacct
    11281 tgcatttcaa tattgtcttc tttggccatc taatcctctg gggtatcact accgtaattt
    11341 gaaccggcaa gataatgccg aagttctgta aataagtaaa gatttgcgcg ctaaatcgca
    11401 acaaacaggt tcggcacatt actccgaaaa cacacggcta agccgcacca aaagcgcaac
    11461 gtataaggga gcggtgagat aaacgatggg cgttacctga cgcgaaaaat tccttatcgg
    11521 cagcggggta atgagcgtaa ccaactctgc gaccgcaatt ataacactct ggggagaaat
    11581 gtgccgaaaa cattcattct tgtggtgaaa acaagcaccg tggtacccag aaattattcg
    11641 gcaatcgtcc gaggcgcatt tgattgagat aattaaggta atcccggcgg ggaatttcgc
    11701 aggcaccaag cgatgctgtg tgatcgttaa ggacctggca gtcgataagc ttaccgccat
    11761 gaccgataaa ttcctcacag aataccagaa gcgccgtttt agacgcattt tccatccggc
    11821 tgaacatgga ctcgccacaa aatagtgttc cctgggccac gccgtacata ccgccgacaa
    11881 gctcatcttc acgccagact tcaatggagt gggcatgacc gagttcgtga aggcgatggt
    11941 aggcttcgac cacgccacgc gtgatccagg ttccttcttc gcgatcgctg gcacagcctt
    12001 caatgacctg accaaaagcg taattcatcg tgacacgata gggcgagcgt ttatgaaatc
    12061 gcttcatact acggctgata tgcagtgatt ctggccatag caccgcgcgg ggatcgggcg
    12121 accaccagag gatggggtcg cctggagaaa accacggaaa aataccacgc tggtaagcca
    12181 ttaacaggcg cgcagggcta agatcgcccc caagtgccag caggccgtta ggctcacgta
    12241 atgcgccttc cggggaaggg aaggctattg aatggcgaga aagctgaacc aggcgcatga
    12301 ccgcaaaact ccacgcaagt cggatcgttc aataatagct tacaaaccct gcttgaactg
    12361 gtaataacgc ccctgtctgg caagcagttc tgcgtgagta ccttgctcaa taatttgccc
    12421 gttgtccatc actattattt gttggaaacg agagagtccg cgaagtcgat gggtgaccat
    12481 taacaccgtt ttctcacgca tcatttctgc aagcaattca aggatctggc tttcggttgt
    12541 ggcatctaag ccttcggtag gttcatccag caacaccagt ggcgcatcat gtaacagcgc
    12601 acgggcgata gccagacggc gcagttcacc accggagagc tggcgtccgc cttcacctaa
    12661 ccaactgttg agacctgcat cctcgagcag cttctccaga ccaacgcgac gcaagatctc
    12721 cgacagagcc tcatcactac tgccaggcga ggcgagtaac agattatcac gcagcgtggc
    12781 gctaaacaga tgcactcgct gaggaacaac gctgatggtc tgtcgtagag ccgcttcatt
    12841 caggctggct atggggctat cgttaagcaa aatctcgccc tgttgcgggt cccaagcgcg
    12901 ggtcagcagt tgtaacagtg ttgatttgcc gcatccggtt cgcccgagaa tcgcaatatg
    12961 ttccccggcg tttacctgaa gagaaatccc tttaagtgcc tgttgggatt gctccggata
    13021 agtgaactga acatcccgta atgtcagcga aacgcgatcg gcaacacgag tttgggtatc
    13081 agggaaggtg acttccggtt tttgatccgt taaatcagtg atccgaacgg cagaggcaat
    13141 gacttgcccc agatgctgaa atgcacccgt tactggtgcc agtgcttcaa acgcggctaa
    13201 cgcgcagaag acaaacaggg caattaacgc gccgggttga gcattgccgc caacgccgcc
    13261 agacgccatc cacagcatca ggatcaccgc taacgcgcca atgagcagca ttatcgcttg
    13321 cgacaatgcg gtcagttcag attgacggcg ttgcgcttcc agccattgaa tttctgtatt
    13381 ctctagttgc gtgcgataac gatcgctggc accaaaaatg gtcagctcag cttgcccttg
    13441 cagccaggcc gtcagttgtt ggcgatactg tccgcgaaga tgagtcagat tttgcccggt
    13501 gctttttccc gcacgataaa acagcggtgg catcaggaaa agcgtcagta acataatgcc
    13561 gcccagcgta aaggcgaggg tgaaatcaag gaaacttaac ccgattgtca ccaccataat
    13621 caccacaaaa gcgcccacca gcggcgagat aacgcgcagg taaagatgat cgagcgtatc
    13681 aacatccgcc accacgcgat tgagcaattc gccctgacga tagcgcgcca gtccggcagg
    13741 ggagaggggc agcaatttgc tgaaggtgta aatgcgcaga tgctgcaaca cgcggaaagt
    13801 cgcgtcgtga cttaccagac gttcaaaata gcgcccggca gtacgggtga ttgctgcgcc
    13861 acgcacgccc gcagcgggta gcatatagtt gaagctgtac agtccggcaa cccccgcaac
    13921 cgctgaggcc gagaggaacc agccggaaag tgtcaacaga ccgatactgg cgagcagcgt
    13981 cacaattgcc agcacaatac caagacttaa catccattta tgacgtttat acagtgccag
    14041 atagggtagc aaagcgcgca tttaaatctc ctcctgacga tgggccagta atgtggcgaa
    14101 tgggccacca gccacactta attccgcgta acgtccttgc tcaataatcc ggccatcctg
    14161 cataacccaa atgacatccc agtcagcaag atcttctaac tggtgggtga ccattaacgt
    14221 tgtctggcgc agagaggcgg cattcagcgc ctccattacg cgctgttcac tgtgagcatc
    14281 aaggctggca gcgggttcat ccaacagtaa tagcgaacag ggatttagta acgcacgggc
    14341 caccgccacg cgctgcgcct gccccacgga aaggcgggca gcctggtcgc caacaggcgt
    14401 atcaacgcct tgtggcagga gcggtagaaa ctcgctgacc caggcgttat ccagcgctgc
    14461 ttgtaattct tgttcgctgg catcaggtcg cgccagtagt acgttatccc gtaatgttgc
    14521 tgccggtaat tgtgggtttt gcccaaccca ggagagatgt ttacgccatg attctggtga
    14581 taaatcgcgt aattctatcc cgttgattcg taatgatccc tgatatgaga gaaaaccaga
    14641 aagcgcgttc agcagtgagc ttttacctga accgctgcga ccaaccaaca ctgcacgttg
    14701 gcctgctggc aaagtaaagt tcagcggtcc ggccagcgtt ttaccttccg gcgacgtgat
    14761 aaacagatcc tcggcctcaa tggtcagcgg atcggtcaat gctaattccg cctcaccgcg
    14821 ctgcggatgg gcgagcgggg tttccataaa cgttttcaga ctgtcagctg caccaacagc
    14881 ctgggcttta gcatgataaa acgtaccgag atcgcgtaat ggctggaaaa actctggcgc
    14941 aaggatcagg gccagaaaac ccgcagccag cgtcacgcca gtatcgtagt gaccaaaatc
    15001 cagctcgccg agataggaaa aaccaaagta gaccgccacc agagcaattg acagcgaggt
    15061 aaaaaattcg agaatgccgg aggataaaaa cgccagtcgt agcacttcca ttgtccgttg
    15121 gcggaaatct tccgaagcag aacgaatact ttcaatttca gcttcaccac gaccaaaaat
    15181 acgcaatgtt tccatgccgc gcaggcgatc gaggaaatgc ccacttaagc gagcaagagc
    15241 gagaaagtta cgtcggttag catcggcagc ccccattcca accagcgcca taaacaacgg
    15301 aattagcggt gctgtgccca gcagaatgag cgccgcagcc cagttagagg ggaagatcgc
    15361 caccacaatc agcaacggca ccgacactgc cagcgccatt tgcggcagat agcgtgcata
    15421 gtaatcatgc atatcgtcaa tttgctcgag taccagcgtc gcccagctcc ccgcaggttt
    15481 accctgaatc cacgctggcc ctgcttgttg cagacggtcg agaacctgac ggcggatggc
    15541 aaagcggata tgctgcccgg cgtgataacc cacccgttcg cgtaaccaga ccacccatgc
    15601 gcgcagtaca aaggtcagaa ccagtaacgt aaagggaagc agcagggctt cacggggaat
    15661 attctccata atcatatgtt gcagaattcg cgccatgaac caggcctggg caatgatcaa
    15721 tatgccgctc acaaagccca gcagacgaga aatattcagc caacgttggg agatgacgct
    15781 ttgctgtttt aaccagcggg ttaactcttt ttgacgagat ttattcattg cacgcttagc
    15841 aggtgagtta tcagaattat ttgcagagca atgttacaac ggggaaaaaa taaaggcgac
    15901 ccatagtcgc atggtgtcgc cttctttact tttgttactg atttgtaaaa ttattttgcg
    15961 tcagctaaac catcgaggta gcgttccgca tcaagtgctg ccatgcagcc tgtaccggcc
    16021 gaagtaatgg cctggcgata aatgtgatcc atcacgtcgc ctgcggcaaa gacgccagga
    16081 atgctggtct gggtggcatt accatgaata cccgactgta ctttgatgta gccgttttcc
    16141 agttccagct gcccttcgaa aatcgcagta ttcgggctgt gaccgatagc aacaaacaga
    16201 ccggcaacgt cgagtgactc gatgttatcg ctgttttgcg tatcgcgcag acgaacgcca
    16261 gtgacaccca tttgatcgcc ggtcacttct tccagcgtac ggttggtgtg cagaatgatg
    16321 ttgccgttct ccactttatc catcaggcgc ttaatgagga ttttttccgc gcggaaaccg
    16381 tcacggcggt gaatcagatg cacttccgaa gcgatgttag acagatacag cgcctcttca
    16441 accgcggtat tgccgccgcc gatgaccgca actttctggt tgcgatagaa gaaaccgtcg
    16501 caggttgcac aagcagaaac cccacggcct ttaaaggctt cttcagaggg caggccgaga
    16561 tagcgtgcag aagctccggt ggcaataatc agcgcgtcgc aagtgtattc gccgttatcg
    16621 ccattcagac ggaacggacg gttttgcaga tccaccttgt tgatatgatc aaaaatgatc
    16681 tcagtttcaa acttggtggc atgttcgtgc atgcgctcca ttaataacgg accggtcaga
    16741 tcgtttggat cgccaggcca gttttccact tccgtggtgg tggtcagttg gccgcctttt
    16801 tccatgccgg taatcagcac aggttgcagg ttggcgcgcg ccgcgtagac agcagcggtg
    16861 tatcccgccg ggcctgaacc caggataagc agtttactgt gtttggtcgt gcccatgaga
    16921 tccccatagt tgttggcaga caatgggcag gattgtaggg aatttacaga cgtaaaaaaa
    16981 gagtatgacg attttgttaa caatttgtgc aatcggcagc atcgataagc aggtcaaatt
    17041 ctcccgtcat tatcacctct gctacttaaa tttcccgctt tataagccga ttaaatgatg
    17101 aataaacgcc cctgttaatg aatatctggc atgttgtact aaaaatcgat gttttgcttt
    17161 gacaatcccc tggtgttttg cgaaaacatt cgaggaagaa aaaaaacagt attcttatat
    17221 gcgcataacc atgcatgtaa ataccatgtt taccgtgcta gtgaaatcta cgtatggcgt
    17281 ggacagacgc cattcgtgat gtcgatagct gccacaaggc aacggtcttc tcaccgtaga
    17341 cccaggcatt gcgcgccgtg aatcttcatg atttcggtct atcgtgacgg gtagcgactc
    17401 tgaacagtga tgtttcaggg tcagacagga gtagggaagg aatacagaga gacaataata
    17461 atggtagata gcaagaagcg ccctggcaaa gatctcgacc gtatcgatcg taacattctt
    17521 aatgagttgc aaaaggatgg gcgtatttct aacgtcgagc tttctaaacg tgtgggactt
    17581 tccccaacgc cgtgccttga gcgtgtgcgt cggctggaaa gacaagggtt tattcagggc
    17641 tatacggcgc tgcttaaccc ccattatctg gatgcatcac ttctggtatt cgttgagatt
    17701 actctgaatc gtggcgcacc ggatgtgttt gaacaattca ataccgctgt acaaaaactt
    17761 gaagaaattc aggagtgtca tttagtatcc ggtgatttcg actacctgtt gaaaacacgc
    17821 gtgccggata tgtcagccta ccgtaagttg ctgggggaaa ccctgctgcg tctgcctggc
    17881 gtcaatgaca cacggacata cgttgttatg gaagaagtca agcagagtaa tcgtctggtt
    17941 attaagacgc gctaacacgg aacaggtgca aaatcggcgt attttgatta cactcctgtt
    18001 aatccataca gcaacagtac tggggtaacc tggtactgtt gtccgtttta gcatcgggca
    18061 ggaaaagcct gtaacctgga gagcctttct tgagccagga atacactgaa gacaaagaag
    18121 tcacattgac aaagttaagt agcggccgcc gccttctgga ggcgttgctg atccttattg
    18181 tcctgtttgc cgtctggttg atggctgcct tactaagctt taacccttcg gaccccagct
    18241 ggtcgcaaac ggcctggcat gaacctatcc ataatttagg tgggatgccc ggtgcgtggt
    18301 tggcagatac gctgttcttt atttttggcg tgatggctta caccattccc gtcattattg
    18361 tcggcggttg ttggtttgcc tggcgtcatc agtccagcga cgaatacatt gattattttg
    18421 ccgtttcgct acgcatcatt ggcgttttgg cgctcatcct tacctcctgt ggtctggcgg
    18481 caatcaacgc tgacgatatc tggtattttg cctccggtgg cgtcattggc agcttactaa
    18541 gcactacgct acaaccactg ctacacagta gcgggggaac tattgcgctg ctctgcgttt
    18601 gggcagcggg cctgacgttg ttcaccggtt ggtcatgggt gaccattgct gaaaaactcg
    18661 gcggctggat tttaaacatt ctcaccttcg ccagtaatcg tacccgtcgc gatgatacct
    18721 gggtcgatga agatgagtat gaagacgacg aagagtatga agatgaaaat cacggcaaac
    18781 agcatgaatc acgccgtgcc cgtattcttc gcggcgcgct agcgcgtcgt aaacggttgg
    18841 cggaaaaatt cattaatccg atggggcggc aaacagacgc tgcgttgttc tccggtaagc
    18901 ggatggatga tgacgaagag attacctaca ttgcacgcgg tgtggctgcc gacccggacg
    18961 acgtcctatt ttcgggcaat cgtgcaacgc agccagaata tgacgaatac gatccattat
    19021 taaacggtgc gccaattacc gaacctgtcg ctgtagcagc tgctgctacc acggcgacac
    19081 aaagctgggc tgcgccggtt gaacctgtga ctcagacgcc gcctgttgcc tctgttgatg
    19141 ttccacctgc gcaacctaca gtagcctggc agcctgtacc gggtccacaa acgggagagc
    19201 cggttattgc tcctgcaccg gaaggttacc cacagcagtc acaatatgcg cagcctgcag
    19261 tgcaatataa tgagccgctg caacaaccag tacagccgca gcagccgtat tatgcacctg
    19321 cagctgaaca acctgcgcaa cagccgtatt atgcccctgc gccagaacaa ccggtggcag
    19381 gtaacgcctg gcaagccgaa gagcagcaat ccacttttgc tccacagtct acataccaga
    19441 ctgagcaaac ttatcagcag ccagccgctc aggagccgtt gtaccaacag ccgcaacccg
    19501 ttgaacagca gcctgttgtg gagcctgaac ccgttgtaga agagacaaaa cccgcgcgtc
    19561 cgccgcttta ctactttgaa gaagttgaag agaagcgagc ccgtgaacgt gaacaacttg
    19621 cggcctggta tcaaccgatt ccagaaccgg ttaaagaacc agaaccgatc aaatcttcgc
    19681 tgaaagcacc ttctgttgca gcagtacctc cagtagaagc cgctgccgct gtttccccgc
    19741 tggcatctgg cgtgaaaaaa gcgacactgg cgacgggggc tgccgcaacc gttgccgcgc
    19801 cagtcttcag tctggcaaat agcggtggac cgcgtcctca ggtcaaagag gggattggtc
    19861 cgcagttgcc acgaccgaaa cgtatccgcg tgccaactcg tcgtgaactg gcgtcttacg
    19921 gtattaagct gccctcacag cgtgcggcgg aagaaaaagc ccgtgaagcc cagcgcaatc
    19981 agtacgattc tggcgatcag tacaacgatg atgaaatcga tgcgatgcag caggatgaac
    20041 tggcacgtca gttcgcccag acacagcagc aacgctatgg cgaacagtat caacatgatg
    20101 tgcccgtaaa cgcagaagat gcagatgctg cggcagaggc tgaactggct cgtcagtttg
    20161 cgcaaactca acaacaacgt tattccggcg aacaaccggc tggggcgaat ccgttctcgc
    20221 tggatgattt tgaattttcg ccaatgaaag cgttgctgga tgatggtcca cacgaaccgt
    20281 tgtttacgcc aattgttgaa cctgtacagc agccgcaaca accggttgca ccgcagcagc
    20341 aatatcagca gccgcaacaa ccagttccgc cgcagccgca gtatcagcag ccacaacagc
    20401 cggttgcgcc gcagccacaa tatcagcagc cgcaacaacc ggttgcgcca cagcagcaat
    20461 atcagcagcc gcaacaaccg gttgcgccgc agcagcagta tcagcagcca caacagccag
    20521 ttgcgccaca accgcaggat accctgcttc atccgctgtt gatgcgtaat ggcgacagcc
    20581 gtccgttgca taaaccgacg acgccgctgc cttctctgga tttgctgaca ccgccgccga
    20641 gcgaagtgga gccggtagat acctttgcgc ttgaacaaat ggctcgtctg gtggaagcgc
    20701 gtctggctga tttccgtatt aaagccgatg tcgtcaatta ctctccgggg ccggttatca
    20761 ctcgctttga acttaacctg gctcctggcg taaaagcggc gcgcatttct aacttgtcac
    20821 gggaccttgc ccgttcattt tcgacggtgg cggtgcgtgt cgttgaagtt attcctggca
    20881 aaccctatgt aggtctggag ttaccaaata aaaaacgaca aaccgtttat ctgcgcgaag
    20941 ttttggataa cgccaaattc cgcgataatc cgtcgccatt aaccgtggtg ctgggtaaag
    21001 atatcgccgg tgagccggtg gttgccgatc tggcgaaaat gccgcacttg ttggttgcgg
    21061 ggactaccgg ttccggtaaa tctgtcggtg tgaacgcgat gatcctgagc atgctttata
    21121 aagcacagcc agaagatgtg cgtttcatca tgatcgaccc gaaaatgctg gagctttcgg
    21181 tgtatgaagg cattccgcac ctgttaacgg aagtcgttac tgatatgaaa gatgccgcca
    21241 acgcgctgcg ctggtgtgtt aacgagatgg agcgtcggta taaactgatg tctgcgctgg
    21301 gtgtgcgtaa tctggcgggt tataacgaaa aaattgctga agccgatcgc atgatgcgtc
    21361 cgattccaga cccgtactgg aagccgggtg acagtatgga tgcccagcat ccggtgctga
    21421 aaaaagaacc atacattgtg gtgttggttg acgaatttgc cgacctgatg atgacggtag
    21481 gtaaaaaagt ggaagagctg atagcacgtc tggcgcaaaa agcccgtgcc gcgggtatcc
    21541 acctcgtact ggcaactcag cgtccatcgg ttgatgttat tactggtctg attaaagcga
    21601 atattccgac ccgtatcgcc tttaccgtat ccagtaagat tgactcacgt accattcttg
    21661 atcaggctgg cgcggaatca ctgctgggta tgggggatat gctctactct gggccgaact
    21721 ccacgttgcc ggtacgtgtc catggtgctt ttgttcgcga tcaggaagtt catgccgtgg
    21781 tgcaggactg gaaagcgcgt ggtcgcccac agtatgttga tggcatcacc tccgacagcg
    21841 aaagcgaagg tggtgcgggt ggtttcgatg gcgctgaaga actggatccg ttgttcgatc
    21901 aggcggtgca gtttgtcact gaaaaacgca aagcgtcaat ttctggcgta cagcgtcagt
    21961 tccgcattgg ttataaccgt gcagcgcgta ttatcgaaca gatggaagcg caggggattg
    22021 tcagcgaaca ggggcacaac ggtaatcgtg aagtgctggc cccaccgccg tttgactaat
    22081 taatgcattg ccggataagg cgcggtagcg tcgcatccgg cactctatca actgaaaatt
    22141 cagtattttc ttctttcctc aagctgatta ttagcctgga atagagagta gagggaactc
    22201 ccgatcggga gtgacgtaat ttgaggaata atgatgaaaa aaattgccat cacctgtgca
    22261 ttactctcaa gcttagtagc aagcagcgtt tgggctgatg ccgcaagcga tctgaaaagc
    22321 cgcctggata aagtcagcag cttccacgcc agcttcacac aaaaagtgac tgatggtagc
    22381 ggcgcggcgg tgcaggaagg tcagggcgat ctgtgggtga aacgtccaaa cttattcaac
    22441 tggcatatga cacaacctga tgaaagcatt ctggtttctg acggtaaaac actgtggttc
    22501 tataacccgt tcgttgagca agctacggca acctggctga aagatgccac cggtaatacg
    22561 ccgtttatgc tgattgcccg caaccagtcc agcgactggc agcagtacaa tatcaaacag
    22621 aatggcgatg actttgtcct gacgccgaaa gccagcaatg gcaatctgaa gcagttcacc
    22681 attaacgtgg gacgtgatgg cacaatccat cagtttagcg cggtggagca ggacgatcag
    22741 cgcagcagtt atcaactgaa atcccagcaa aatggggctg tggatgcagc gaaatttacc
    22801 ttcaccccgc cgcaaggcgt cacggtagat gatcaacgta agtagaggca cctgagtgag
    22861 caatctgtcg ctcgattttt cggataatac ttttcaacct ctggccgcgc gtatgcggcc
    22921 agaaaattta gcacagtata tcggccagca acatttgctg gctgcgggga agccgctgcc
    22981 gcgcgctatc gaagccgggc atttgcattc gatgatcctc tgggggccac cgggtaccgg
    23041 caaaacaacc ctcgctgaag tgattgcccg ctatgcgaac gctgatgtgg aacgtatttc
    23101 tgccgtcacc tctggcgtga aagagattcg cgaggcgatc gagcgcgccc ggcaaaaccg
    23161 caatgcaggt cgccgcacta ttctttttgt tgacgaagtt caccgtttca acaaaagcca
    23221 gcaggatgca tttctgccac atattgaaga cggcaccatc acttttattg gcgcaaccac
    23281 tgaaaacccg tcgtttgagc ttaattcggc actgctttcc cgtgcccgtg tctatctgtt
    23341 gaaatccctg agtacagagg atattgagca agtactaact caggcgatgg aagacaaaac
    23401 ccgtggctat ggtggtcagg atattgttct gccagatgaa acacgacgcg ccattgctga
    23461 actggtgaat ggcgacgcgc gccgggcgtt aaatacgctg gaaatgatgg cggatatggc
    23521 cgaagtcgat gatagcggta agcgggtcct gaagcctgaa ttactgaccg aaatcgccgg
    23581 tgaacgtagc gcccgctttg ataacaaagg cgatcgcttt tacgatctga tttccgcact
    23641 gcataagtcg gtacgtggta gtgcacccga tgcggcgctg tactggtatg cgcgaattat
    23701 taccgctggt ggcgatccgt tatatgtcgc gcgtcgctgt ctggcgattg cgtctgaaga
    23761 cgtcggtaat gccgatccac gggcgatgca ggtggcaatt gcggcctggg attgctttac
    23821 tcgcgttggc ccggcggaag gtgaacgcgc cattgctcag gcgattgttt acctggcctg
    23881 cgcgccaaaa agcaatgctg tctacactgc gtttaaagcc gcgctggccg atgctcgcga
    23941 acgcccggat tatgacgtac cggttcattt gcgtgatgcg ccgacgaaat taatgaagga
    24001 aatgggctat gggcaggaat atcgttacgc tcatgatgaa gcaaacgctt atgctgccgg
    24061 tgaggtttac ttcccgccgg aaatagcaca aacacgctat tatttcccga caaacagggg
    24121 ccttgaaggc aagattggcg aaaagctcgc ctggctggct gaacaggatc aaaatagccc
    24181 cataaaacgc taccgttaat gttatcgttg cggtaatgtt gttactgtat ccctgtggtc
    24241 gcaggctgtg gccacatctc ccatttaatt cgataagcac aggataagca tgctcgatcc
    24301 caatctgctg cgtaatgagc cagacgcagt cgctgaaaaa ctggcacgcc ggggctttaa
    24361 gctggatgta gataagctgg gcgctcttga agagcgtcgt aaagtattgc aggtcaaaac
    24421 ggaaaacctg caagcggagc gtaactcccg atcgaaatcc attggccagg cgaaagcgcg
    24481 cggggaagat atcgagcctt tacgtctgga agtgaacaaa ctgggcgaag agctggatgc
    24541 agcaaaagcc gagctggatg ctttacaggc tgaaattcgc gatatcgcgc taaccatccc
    24601 taacttgcct gcagatgaag tgccggtagg taaagacgaa aatgacaacg ttgaagtcag
    24661 ccgctggggc accccgcgtg agtttgactt tgaagttcgt gaccatgtga cgctgggtga
    24721 aatgcactct ggcctcgact ttgcagctgc agttaagctg actggttccc gctttgtggt
    24781 aatgaaaggg cagattgctc gcatgcaccg cgcactgtcg cagtttatgc tggatctgca
    24841 taccgaacag catggctaca gtgagaacta tgttccgtac ctggttaacc aggacacgct
    24901 gtacggtacg ggtcagctgc cgaaatttgc tggcgatctg tttcatactc gtccgctgga
    24961 agaagaagca gacaccagta actatgcgct gatcccaacg gcagaagttc cgctgactaa
    25021 cctggtgcgc ggtgaaatca tcgatgaaga tgatctgcca attaagatga ccgcccacac
    25081 cccatgcttc cgttctgaag ccggttcata tggtcgtgac acccgtggtc tgatccgtat
    25141 gcaccagttc gacaaagttg aaatggtgca gatcgtgcgc ccggaagact caatggcggc
    25201 gctggaagag atgactggtc atgcagaaaa agtcctgcag ttgttgggcc tgccgtaccg
    25261 taaaatcatc ctttgcactg gcgacatggg ctttggcgct tgcaaaactt acgacctgga
    25321 agtatggatc ccggcacaga acacctaccg tgagatctct tcctgctcca acgtttggga
    25381 tttccaggcg cgtcgtatgc aggcacgctg ccgcagcaag tcggacaaga aaacccgtct
    25441 ggttcatacc ctgaacggtt ctggactggc tgttggtcgt acgctggttg cagtaatgga
    25501 aaactatcag caggctgatg gtcgtattga agtaccagaa gttctgcgtc cgtatatgaa
    25561 cggactggaa tatattggct aatacccaat ttttctgaat ctaaaaagcg cctgcgggcg
    25621 ctttttttgt ctccctttga taccgaacaa taattactcc tcacttacac gtaatactac
    25681 tttcgagtga aaatctacct atctctttga ttttcaaatt attcgatgta tacaagccta
    25741 tatagcgagc tgctatagaa ataattacac aatacggttt gttactggaa tcaatcgtga
    25801 gcaagcttga gtgagccatt atgaaaacga aaatccctga tgcggtattg gctgctgagg
    25861 tgagtcgccg tggtttggta aaaacgacag cgatcggcgg cctggcaatg gccagcagcg
    25921 cattaacatt accttttagt cggattgcgc acgctgtcga tagcgccatt ccaacaaaat
    25981 cagacgaaaa ggttatctgg agcgcctgta cagttaactg tggtagtcgc tgcccgctac
    26041 gtatgcacgt cgtggacggt gaaatcaaat atgtcgaaac ggacaatacc ggcgatgaca
    26101 attacgacgg cctgcaccag gttcgcgcct gcctgcgtgg gcgttccatg cgtcgccgtg
    26161 tctacaatcc ggaccgcctg aaatatccga tgaaacgagt cggggcgcgc ggtgaaggca
    26221 aattcgagcg cattagctgg gaagaagcct acgacatcat cgcgaccaat atgcagcgcc
    26281 tgatcaaaga gtacggcaac gagtctatct atctgaacta tggcaccggt acgctgggcg
    26341 gcaccatgac ccgctcctgg ccgccgggaa ataccctggt cgcgcggctg atgaactgct
    26401 gcggcggcta tctgaaccat tacggcgact actcctccgc gcaaattgcg gaaggtttga
    26461 actataccta cggcggctgg gcagatggca acagcccgtc ggatatcgaa aacagtaagc
    26521 tggtagtgct gtttggtaat aaccctggcg aaacgcgaat gagtggcggt ggggtgactt
    26581 actatcttga acaggcacgc cagaaatcta atgcccgcat gatcatcatc gatccgcgct
    26641 ataccgacac cggtgccggg cgcgaagatg agtggatccc tattcgtccg ggaacagatg
    26701 ccgcactggt taacggtctg gcgtacgtca tgatcactga aaacctggtg gatcaggcat
    26761 tcctcgataa atattgcgtt ggctacgatg agaaaaccct gccagccagt gcgccgaaaa
    26821 atggccacta taaagcttat attctgggtg aagggccaga tggcgtggct aaaacgccgg
    26881 aatgggcctc gcaaatcact ggtgttccgg cagacaaaat cgtcaaattg gctcgtgaaa
    26941 tcggtagtac caaaccggcg tttatcagcc agggatgggg cccgcagcgt cacgctaacg
    27001 gtgaaatcgc aacccgtgct atctcgatgc tggcgattct gaccggtaac gttggtatta
    27061 acggaggcaa cagcggcgcg cgtgaaggtt catacagctt accgtttgtc cgtatgccga
    27121 ccttggaaaa cccgatccag accagcattt cgatgtttat gtggaccgat gccattgaac
    27181 gtggcccgga aatgacggcg ctgcgtgatg gtgtacgcgg gaaagataag ctggatgtgc
    27241 cgatcaaaat gatctggaac tatgccggta actgcctgat taaccagcat tctgaaatca
    27301 accgtaccca tgaaatcctt caggatgata agaagtgcga gctgattgtg gttatcgact
    27361 gccacatgac ctcatcggcg aaatatgctg acatcctgct gcctgactgc accgcttccg
    27421 aacagatgga ctttgcgctg gatgcatcct gcgggaatat gtcttacgtg attttcaacg
    27481 atcaggtgat taaaccgcgc tttgaatgta agaccatcta tgaaatgacc agcgaactgg
    27541 caaaacgtct tggcgttgag caacagttta ctgaaggccg tacccaggaa gagtggatgc
    27601 ggcatctgta tgcccagtcg cgggaagcga ttcctgaact gccaacgttt gaagagttcc
    27661 gcaagcaggg gatctttaaa aagcgcgacc cacaagggca tcacgttgct tataaagcct
    27721 tccgtgaaga tccgcaggca aacccactga ctacgccatc gggcaaaatt gagatttatt
    27781 cgcaggcgct ggctgacatt gccgctacct gggaattgcc tgaaggcgat gtgatcgatc
    27841 cactgccgat ctacacgccg ggctttgaaa gttatcagga tccgctgaac aaacagtatc
    27901 cgctgcagct tacaggtttc cactataaat ctcgcgttca ctcaacttac ggcaacgttg
    27961 atgtgctgaa agcggcttgc cgtcaggaaa tgtggatcaa cccgcttgat gcccaaaaac
    28021 gcggtatcca caacggcgat aaagtcagga tctttaacga tcgtggtgag gttcatattg
    28081 aggcgaaagt gacgccacga atgatgccgg gtgtagtcgc actgggtgaa ggtgcctggt
    28141 atgacccgga tgcaaaacgt gtcgataagg gtggttgtat taacgtactg accactcaac
    28201 gtccgtctcc tctcgctaag gggaatccgt cacatacaaa ccttgttcag gttgaaaagg
    28261 tgtaaggagt aaccgatgac aacccagtat ggatttttta ttgattccag ccgttgcacc
    28321 ggttgcaaaa cctgcgagct ggcttgtaaa gactacaaag atttgacgcc agaagtcagc
    28381 ttccgccgca tttatgaata tgctggcggc gactggcagg aagataacgg tgtctggcac
    28441 cagaacgtgt ttgcctacta tctgtcgatt tcgtgcaacc actgcgaaga tccggcttgt
    28501 actaaagtct gcccgagcgg tgcgatgcat aaacgtgaag atggttttgt tgtggtcgat
    28561 gaagatgtgt gcattggctg ccgctactgc catatggctt gcccgtatgg cgcaccgcaa
    28621 tataacgaaa cgaaaggcca tatgaccaaa tgcgatggtt gttatgaccg tgttgcggag
    28681 ggtaaaaagc cgatctgtgt tgaatcttgt ccgctgcggg cgctggattt cggccctatc
    28741 gacgagctgc gtaaaaaaca tggcgatctg gcggccgttg cgccgttgcc gcgagctcac
    28801 tttaccaaac cgaatattgt gatcaaaccc aatgccaata gccgcccgac cggggatacc
    28861 actggctatc tggcaaaccc gaaggaggtg tgagatggga agtggatggc atgaatggcc
    28921 gctgatgatc ttcacggtct tcgggcaatg tgtagcaggt ggttttatcg ttctggcttt
    28981 ggcgctgctc aaaggcgacc tgcgagcaga agcccagcag cgtgttatcg cctgcatgtt
    29041 tggtttatgg gtgctgatgg gcattggctt tatcgcctct atgctccatc ttggttcacc
    29101 aatgcgcgct tttaactcgc tcaaccgggt aggggcttca gcactcagta acgaaatcgc
    29161 cagcggttcg atcttttttg ccgtaggcgg catcggctgg ttgctggcaa tgctgaaaaa
    29221 gctgtcaccg gcattgcgta cgctgtggct gatagtgacg atggttcttg gcgtcatctt
    29281 tgtctggatg atggtgcgtg tgtataacag cattgatacc gttccgacct ggtacagcat
    29341 ctggacgccg atgggcttct tcctgacgat gtttatgggc ggcccgttac tgggttacct
    29401 gttgttgagt ctggctggcg tcgatggctg ggcgatgcgt ctgctgccag caatttctgt
    29461 actggcactg gtagtgagtg gcgtggtgtc agtgatgcag ggcgcagagc tggcgactat
    29521 tcatagttct gtgcagcagg ccgcagcgct ggtgccggac tatggtgcgc tgatgtcctg
    29581 gcggatcgtg cttttggccg ttgccctgtg cttgtggatt gcaccacagc taaaaggtta
    29641 tcagcctgcg gttccgctac tttctgtatc attcattctg cttctggcag gggaattaat
    29701 cggtcgcggc gtattctatg gtttgcatat gaccgtgggg atggccgtcg caagctaatc
    29761 ataacaaccg gggtttcggc cccggttctc ttttatttct gcttcgttaa cgtgtcataa
    29821 ctggtcatca aattacgata atcaggaatg tggttagaga acaacgtcgc cagtccttca
    29881 atatcattac gccagtcgcg atgcagctcg cacgccacgc cgaaccatgt catcaattgc
    29941 gcaccagctt gcgacaagcg atcccatgcc gaatgccggg taatttcatt aaaggtccct
    30001 gacgcgtcag tcacgacaaa gacgtcaaac ccttcttcaa tggctgaaag cgccgggaat
    30061 gccacgcaaa cttcggttac cacaccggca ataattaact gttttttacc tgtcgctttg
    30121 acagctttta caaaatcttc gttatcccag gcgttaatat ttcccgggcg agcaatgtaa
    30181 ggtgtatcgg ggaattgtgc tttcagttct ggaactaatg ggccgttagg accggtttca
    30241 aaactggtgg taagaatagt tggtaagttg aaatacttag ccaggtcacc cagcgccagc
    30301 acgttatttt taaacttatc gggttcgata tcccgtacaa gggaaagtaa accagcctgg
    30361 tgatcaacaa gcaaaacggc agcatcattt ttatcaagac gaacatacgg tttggtcatc
    30421 ctcttctcct ttcgagagtc agtggtacgt ctgaaatcga tcagacgcga tgcattgctc
    30481 tgaaagcata gacgggaaat atgagtttgc tgtgaccatg aaatttttcg actgaacgaa
    30541 gcgtatgaaa tttgtgttag ttcaataaaa acaatcagat gagactaatc atctcttcag
    30601 cattagcgtt atgaatgagt aatataaatc gataataccg ccgctgggat atcgcgtatt
    30661 ttcacccatt gacaatgttt ttggcggtgg catgatgcgc atgaaatttg aacttcctca
    30721 cggttttaat tcatgtccac gtatacccag cctgtcatgc ttttgctgtc tggcctgctt
    30781 ttgttgactc tggcgattgc ggtgttaaat acactcgtgc cgctttggct cgcccaggaa
    30841 cacatgtcca catggcaggt aggcgttgtc agctcatcct attttaccgg caaccttgtc
    30901 ggtacattgc tgacagggta tgtcattaag cgcattggct ttaaccgcag ctattatctg
    30961 gcctccttca tttttgccgc tggctgtgcc ggccttggcc tgatgattgg attctggagc
    31021 tggttggctt ggcgttttgt cgcgggcgtc ggctgtgcca tgatttgggt ggttgttgag
    31081 agcgcgctga tgtgcagtgg gacgtcacgt aaccgtgggc gtttgcttgc tgcgtatatg
    31141 atggtttatt acgtgggaac gtttttaggc cagttactgg tcagcaaagt ttcaaccgag
    31201 ctgatgtccg tattgccgtg ggttacaggt ttgacgttgg cagggatctt accgctgttg
    31261 tttacgcgtg tgctgaatca gcaggctgaa aaccatgatt cgacgtcaat tacgtcaatg
    31321 ctaaaactcc gtcaggcgcg gcttggcgtg aatggctgca ttatctcagg aatcgttctg
    31381 ggatctctat atggcctgat gccgctgtac ctcaatcaca aaggggtgag caatgccagc
    31441 attggtttct ggatggcggt actggtcagt gcgggtatcc ttggacaatg gccgattgga
    31501 cgtctggcgg ataagtttgg tcgactgctg gtgttgcgtg ttcaggtctt tgtcgtcatt
    31561 ctcggcagta tcgcgatgct tagccaggcg gcgatggccc cagcgttatt catcctcggt
    31621 gccgctggct ttacgctata tccggtggcg atggcatggg cttgcgagaa agttgaacat
    31681 catcaactgg tggcgatgaa ccaggcctta ctgttgagct atactgtggg aagtctgctt
    31741 ggcccgtcat ttaccgctat gctaatgcag aatttctccg ataatttatt gtttatcatg
    31801 atcgccagcg tatcgtttat ctatttgctg atgctgctgc gcaacgccgg tcatacgccg
    31861 aaacccgttg ctcacgtgta aatgaattca agcagagtgt gaacttactg tttcacactc
    31921 tgcttttttg tttcttctat ctgacttgct ttattccaaa ttttattcgt ttaaaaataa
    31981 aatgtgcagc aggttataat tttgcatttc gctatttccg cacttcttat ttgccgcgca
    32041 taatccctcg ttttacagat gcccctttaa ttttggcgaa ggatttgtct atggctggga
    32101 atgttcagga aaaacagttg cgatggtaca acattgcgct gatgtctttt atcactgtct
    32161 ggggttttgg caacgttgtt aataactatg ccaaccaggg gctggtggtt gttttttcat
    32221 gggtgtttat ctttgcactc tatttcacac cttatgcgct aattgttggt cagttaggct
    32281 cgaccttcaa agatgggaag ggcggggtca gtacctggat taaacacacg atgggacccg
    32341 gactggctta tctcgccgcg tggacctact gggtggtgca tattccctat ctggcacaaa
    32401 aaccccaggc aattctgatt gcgctcggtt gggcgatgaa aggcgacggt tcgctaatca
    32461 aagaatattc agtcgtagcg ttacaggggt taacgctggt gctgtttatc ttctttatgt
    32521 gggttgcttc acgcggtatg aaatcgctga aaatcgtcgg ttctgtggca gggattgcta
    32581 tgtttgttat gtcgctcctg tatgtggcga tggcggtaac cgcgcctgca attactgaag
    32641 tgcatattgc gaccacaaac attacctggg aaacgttcat tcctcatatc gactttacct
    32701 acattaccac tatttcaatg ctggttttcg cggttggcgg agcagagaag atttctcctt
    32761 acgttaatca aacgcgcaac ccaggaaaag aatttccaaa agggatgtta tgcctggcgg
    32821 tgatggttgc ggtttgtgcc attctgggct cgctggcgat ggggatgatg tttgattcgc
    32881 gtaatatccc ggatgactta atgaccaacg gtcagtatta cgcctttcag aagctgggcg
    32941 agtattacaa catgggtaat actttaatgg tgatttacgc cattgcgaat accctgggac
    33001 aagtagcggc gctggtattc tcgattgatg ccccgcttaa agtgctatta ggtgatgctg
    33061 acagcaaata tattccagcc agtttatgtc gtaccaacgc ttctggtacg cccgttaatg
    33121 gctattttct gaccctggta ctggtggcga ttctgattat gctgccgact ctcggcattg
    33181 gtgatatgaa caatctctat aaatggctgt tgaaccttaa ttcggtagtg atgccgctgc
    33241 gttatttatg ggtatttgtt gcatttattg cagtcgttcg cttggcgcag aaatataaac
    33301 cagaatatgt ctttattcgt aataagcctc tggcaatgac cgtcgggatt tggtgttttg
    33361 cctttaccgc ctttgcctgt ttgacgggga tcttcccgaa aatggaagcc ttcactgcag
    33421 agtggacctt ccagttggcg ctgaatgttg caacgccgtt tgtgctggta ggattaggac
    33481 tgatattccc gctgctggcg cgtaaagcga atagtaaata attattgtgg tcggtgacgc
    33541 tgactctggc agatctgacc cgtttttaca taatcgagaa atgcgcgcaa gccgcaggac
    33601 atatactggc ggtttggata atagatctgg aagcctggac gctctgtgct ccagtcttcc
    33661 agtacacatt ctaaacgacc agtatcaagg tactccttga tctgttcata gagcagatac
    33721 ccgataccag cacccatcaa aacggcctcc agttcagcat caacatcatc cagaatgata
    33781 tttcctgcga cagcaatttc cagctccttt gcaaattgcc agtggaaagg tttgccgctg
    33841 ggataacgaa aaacaacaca ttgatgatta agcagatcat gaggatggcg gggtttgcca
    33901 tatcttgcaa agtactctgg cgttgctgct acacataatt tcactggcgg gccgatcgcc
    33961 acgctgatca tatctttttc gacgatgcaa ctaagtcgaa cgccagcgtc aaagccctgt
    34021 tggacgatat cgacaagaga gtcatcagtt gtgagctcga ctttaatatc aggatattca
    34081 cgtgtgaaac caaccagtaa agacattaaa aatatacgtg cagctacccg ggcggcattg
    34141 attttgagcg ttcctgtcgg tgtcagacga aaatcattca tctcatctaa catgatctga
    34201 atttcgtcga aagctgggcg caatcgttca tagagattag aacccgcttc cgttaacgat
    34261 acgcttcggg ttgtccgatt gaataagcga attttaagac gttgttccag tgttttaata
    34321 ctatggctaa tggcggacga ggataagcct aactcatcgc ccgctgcacg aaagctttga
    34381 ttacgggcca cggcaaagaa agtggcaaag tcagacatat tcatccgcat tagtgaaaat
    34441 ccttcattaa ctcatcctga atcatcacgt ttacaggatt atgccgcctg cgtaaagtgc
    34501 gctccagaac ttaacgtgga ggtaaaatta tgcagtctga acgtatttat ttggtatggg
    34561 cccatcctcg tcatgattca ttgaccgcac atattgctga tgcgatccat cagcgggcaa
    34621 tggagcggaa aatacaggtg acggaactcg atttatatcg gcgtaatttc aacccagtga
    34681 tgacgccgga agatgaacca gactggaaga atatggataa acgttattct ccagaggttc
    34741 atcagcttta ttcagagctg cttgaacatg acacgttagt ggtggttttt cctctctggt
    34801 ggtacagctt cccggcaatg ctaaaaggat atattgacag agtatggaat aatgggctgg
    34861 cttatggaga tgggcacaaa ttaccattca ataaagttcg ttgggtggcg ctggttggag
    34921 gagacaaaga atcatttgtc cagatgggct gggaaaaaaa tataagcgat tatttaaaaa
    34981 atatgtgcag ttatcttggt attgaagatg ccgatgtcac tttcttgtgt aatacagtgg
    35041 tattcgatgg ggaagaactt cacgcgagct attatcagtc gttattatct caggtacggg
    35101 atatggtaga tgcactataa gatgtgttaa aaacgctgta gcagaatgaa gcgcggaata
    35161 aaaaagcggc aactcaataa agttgccgct ttacggggaa attagaacat taccttatga
    35221 ccgtactgct caagaatgcc tttcacgcgt tccatggtct ctttcttcgg tggtttaaca
    35281 ccgtcgagtt tgtactcttc acccattgcc acccatttgt gtttgcccag ctcgtggtag
    35341 gggagaagct cgattttctc aacgttgccc atatcacggg taaattcacc gaggcgatgc
    35401 gctgaatcgt catcgtcaga ccagcctggg acaacaacgt agcggatcca caccttcaca
    35461 tttttgttcg ccagatattt agcgaactcc agcgtgcggt ggttggaaac tccaaccaga
    35521 ttttggtgga tctcgtcgtt catctgtttg agatcgagca ttaccaggtc ggttacttcc
    35581 agcagttcat caatcaccgg atcgtaacga cgaacaaaac cgttggtgtc cagacaggta
    35641 tgaatgcctt cttttttgca ggcgcggaac cagtcacgaa caaactcagc ttgcaggatt
    35701 gcttcaccgc cggatgcggt aacgccgccg ccggaagcgt tcataaagtg gcgataggtc
    35761 accacttcct tcatcaaatc ttcaacggta acttctttac cgccatgcgt gtcccaggtg
    35821 tcgcggttat gacaatacag gcagcgcatc aggcagccct ggaaaaaggt gataaagcga
    35881 atacccgggc cgtctacggt tccacaggat tcaaaggagt gaatgcgacc aataactgac
    35941 attgcggtgt ttctccagat gtggcccatc tgaggccgtg ttggtgcgca gctcgaaggc
    36001 tacgtcgagt ctgttttggc agtcacctta aagtatagat agctgacaaa aaaggctctc
    36061 gcgctaaaaa aggccccact ttcgtggagc ctttattgta cgctttttac tgtacgattt
    36121 cagtcaaatc taattacata gattgagtga aggtacgagt aataacgtcc tgctgctgtt
    36181 ctttagtcag cgagttgaaa cgtactgcgt agccagatac acggatggtc agctgcggat
    36241 atttttccgg gttttccatc gcgtcgagca gcatttcacg gttcatcacg ttaacgttca
    36301 ggtgctgacc accttcgatg gatgcttcgt ggtggaagta accatccatc agaccagcca
    36361 ggttggtctt acgaacttcg tcgtctttac ccagtgcgtt cggaacgata gagaaggtgt
    36421 aggagatacc atctttagcg taagcaaacg gcagtttagc aacggaagtc agagaggcta
    36481 ctgcaccttt ctggtcacga ccgtgcatcg ggttagcacc cggtccgaac ggcgcgccag
    36541 cacgacgacc gtctggggtg ttacccgttt tcttaccata cacaacgtta gaagtgatgg
    36601 tcagaacaga ctgagtcggg atagcgtcac ggtaggtgtg cagtttctga attttcttca
    36661 tgaaacgttc taccaggtca acagccaggt catctacacg cggatcattg ttaccaaact
    36721 gcgggtattc gccttcgatt tcgaagtcga tagccagacc gtcttcgtca cgaatcggtt
    36781 taactttcgc atatttgatt gcagacaggg agtcagcagc aacggacaga ccggcgatac
    36841 cacacgccat ggtgcggata acgtcacggt cgtgcagcgc catcagagag gcttcgtagc
    36901 tgtacttgtc gtgcatgtag tggatgatgt tcagtgcagt gatgtactgt ttagccagcc
    36961 agtccatgaa gtgatccatg cgctccatca cttcatcata gttcaggaca tcgcctttga
    37021 tcggttcaga cttcggacca acctgcattt tcagtttttc gtcaacgccg ccgttgattg
    37081 cgtacagcat ggttttcgcc aggtttgcac gcgcaccgaa gaactgcatt tgtttaccaa
    37141 cgatcatcgg gcttacgcag caagcaatag cgtagtcatc gttgttgaag tccggacgca
    37201 tcaggtcatc gttctcatac tgcagagaag aggtgtcgat ggacacttta gcggcgaatt
    37261 tcttgaagtt cagcggcagt ttttcagacc acagaatggt catgttcggt tccggagacg
    37321 gacccatggt gtacagggtg ttcaggaaac ggaagctgtt tttggtaacc agggtacgac
    37381 cgtcgaggcc cataccaccg atagattcgg ttgcccagat cgggtcgcca gagaacagtt
    37441 catcgtattc cggagtacgc aggaagcgaa ccatacgcag tttcatgacc aggtggtcaa
    37501 ccatttcctg cgcttcttgt tcggtgatct tgccagcttt caggtcacgt tcgatgtaca
    37561 catccaggaa ggtggaggta cgaccgaagg acattgcagc accgttctga gacttaacag
    37621 cagccaggta gccgaagtaa gtccactgga tagcttcctg agcgttggta gccggaccag
    37681 agatgtcgta gccgtatttc gcagccattt ctttcatctg acccagagcg cggtgctgtt
    37741 cagcgatttc ttcgcgcaga cggatagtct gttccaggtt tacgccgttt tccagatcag
    37801 cctgcagaga agtgaactgt gccagtttgt ctttcatcag gtagtcgata ccgtacagcg
    37861 caacgcgacg gtagtcaccg atgatacggc cacggccata tgcatctggc agaccggtca
    37921 gaacaccaga tttacggcaa cgcaggatgt ccggagtgta aacgtcgaac acgccctggt
    37981 tgtgagtttt acggtattca gtgaagattt ttttgatcat cggatccagt tcgcggttgt
    38041 acgctttgca ggaaccttcg atcattttga taccaccgaa cgggataaga gcacgtttca
    38101 gcggagcttc agtctgcaga ccaacgattt tctcaagctg cttgttgatg tagccagcgt
    38161 cgtgagaggt gatggtggaa gcaacagcgg tgtcaaagtc aactggcgcg tgagtgcggt
    38221 tttccagttt aacgccttcc attactttgt cccacagggt ggtggtcgct tcagtagcgc
    38281 cagccaggaa ggactcgtca ccctcgtacg gagtgtagtt tttctgaatg aagtcacgga
    38341 cgtttacttc attctgccag tcacctttgg taaaaccttc ccaggctgtg gctaactttt
    38401 cattaagctc ggacatgtaa cacctacctt cttaagtgga ttttttattt actgcgtact
    38461 tcgacaacca ttaatggtgg tcgttttcac gcaggtaaat gacccagtat gtcaacccaa
    38521 ccaacaaacc accaccgata atgttgccga tcgtaaccgg aatcaggtta tcagtgatga
    38581 aattcatcac ggtcaggtga gaaaaatttt ccggtgcaga accgactgcg gtccaaaatt
    38641 ccggggatgc gaagtcgcgg attacaatac ccatcgggat cataaacatg tttgcgatac
    38701 tgtgctcaaa accgctggca acaaacatcg cgaccggcag caccataatg aacgctttgt
    38761 ccatcaggct gcggccagaa taactcatcc atactgccag acataccatc aggtttgcca
    38821 ggataccaag acagacggcc tcaataaaag tatggtgcac tttgtggtcg gcggtttgta
    38881 ggacgtttag tccccattga ccatttgcgg tcatatactc gccggaaagc cacattaaaa
    38941 gtacaaacag cagtgcgccg accaggttgc caaaatagac atttagccag tttttcgcca
    39001 actgacccca ggtgatgcgc ccactcgcct tagcaacaac aatcaacacg gtggaagtaa
    39061 agagatcggc tccgcagaca acacaaagaa tcagccccag agagaagcaa atgccgccaa
    39121 ccagttttgc catgccgaag ggcattgtgc ctgtgccagt ggttgctgtg atatagaaga
    39181 cgaatgcgat tgagatgaaa acaccggcgg taatcgccag atagaaagtc ttaagcggat
    39241 gtttcgttgc tttatagaca cccgcctctt cggccacttt ggccattgca gcaggaagta
    39301 aaagatcaaa agggttgtca gctttcacac taactctctc tttattaagt cggcgacgag
    39361 atactaacaa agcattatag atgagaaatt gatatagatc atatctcgcc tggcttatag
    39421 gcccgtaact cgcatggttt t
//
LOCUS       DAPHBJ010000018        39274 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.018, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000018 DAPHBJ010000000
VERSION     DAPHBJ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.018"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(154..1818)
                     /gene="glnS"
                     /locus_tag="RHZ76_000879"
     CDS             complement(154..1818)
                     /gene="glnS"
                     /locus_tag="RHZ76_000879"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HDV2811570.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2086..4032)
                     /gene="nagE"
                     /locus_tag="RHZ76_000880"
     CDS             complement(2086..4032)
                     /gene="nagE"
                     /locus_tag="RHZ76_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HDV2811571.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4365..5165
                     /gene="nagB"
                     /locus_tag="RHZ76_000881"
     CDS             4365..5165
                     /gene="nagB"
                     /locus_tag="RHZ76_000881"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDV2811572.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5225..6373
                     /gene="nagA"
                     /locus_tag="RHZ76_000882"
     CDS             5225..6373
                     /gene="nagA"
                     /locus_tag="RHZ76_000882"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDV2811573.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     KNADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDCKITKTIVNGNEVVTQ"
     gene            6382..7602
                     /gene="nagC"
                     /locus_tag="RHZ76_000883"
                     /gene_synonym="nagR"
     CDS             6382..7602
                     /gene="nagC"
                     /locus_tag="RHZ76_000883"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HDV2811574.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKIICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7650..8402
                     /gene="nagD"
                     /locus_tag="RHZ76_000884"
     CDS             7650..8402
                     /gene="nagD"
                     /locus_tag="RHZ76_000884"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HDV2811575.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8799..10463
                     /gene="asnB"
                     /locus_tag="RHZ76_000885"
     CDS             8799..10463
                     /gene="asnB"
                     /locus_tag="RHZ76_000885"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HDV2811576.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10844..10920
                     /locus_tag="RHZ76_000886"
     tRNA            10844..10920
                     /locus_tag="RHZ76_000886"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10878..10880,aa:Met,seq:cat)
     gene            10929..11013
                     /locus_tag="RHZ76_000887"
     tRNA            10929..11013
                     /locus_tag="RHZ76_000887"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10963..10965,aa:Leu,seq:tag)
     gene            11037..11111
                     /locus_tag="RHZ76_000888"
     tRNA            11037..11111
                     /locus_tag="RHZ76_000888"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11069..11071,aa:Gln,seq:ttg)
     gene            11127..11203
                     /locus_tag="RHZ76_000889"
     tRNA            11127..11203
                     /locus_tag="RHZ76_000889"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11161..11163,aa:Met,seq:cat)
     gene            11251..11325
                     /locus_tag="RHZ76_000890"
     tRNA            11251..11325
                     /locus_tag="RHZ76_000890"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11283..11285,aa:Gln,seq:ctg)
     gene            complement(11479..12654)
                     /gene="ubiF"
                     /locus_tag="RHZ76_000891"
     CDS             complement(11479..12654)
                     /gene="ubiF"
                     /locus_tag="RHZ76_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HDV2811577.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFAVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRHDDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFMRNLGLMAAERAGVLKRQALKYALGL"
     gene            12800..14224
                     /gene="miaB"
                     /locus_tag="RHZ76_000892"
     CDS             12800..14224
                     /gene="miaB"
                     /locus_tag="RHZ76_000892"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDV2811578.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14377..15417
                     /locus_tag="RHZ76_000893"
     CDS             14377..15417
                     /locus_tag="RHZ76_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDV2811579.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15414..15881
                     /gene="ybeY"
                     /locus_tag="RHZ76_000894"
     CDS             15414..15881
                     /gene="ybeY"
                     /locus_tag="RHZ76_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDV2811580.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            15971..16849
                     /gene="corC"
                     /locus_tag="RHZ76_000895"
     CDS             15971..16849
                     /gene="corC"
                     /locus_tag="RHZ76_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HDV2811581.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16874..18412
                     /gene="lnt"
                     /locus_tag="RHZ76_000896"
     CDS             16874..18412
                     /gene="lnt"
                     /locus_tag="RHZ76_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HDV2811582.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(18539..20415)
                     /locus_tag="RHZ76_000897"
                     /pseudo
     CDS             complement(18539..20415)
                     /locus_tag="RHZ76_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
     gene            20870..21778
                     /gene="gltI"
                     /locus_tag="RHZ76_000898"
     CDS             20870..21778
                     /gene="gltI"
                     /locus_tag="RHZ76_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HDV2811583.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPVAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            21948..22688
                     /gene="gltJ"
                     /locus_tag="RHZ76_000899"
     CDS             21948..22688
                     /gene="gltJ"
                     /locus_tag="RHZ76_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HDV2811584.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            22688..23362
                     /gene="gltK"
                     /locus_tag="RHZ76_000900"
     CDS             22688..23362
                     /gene="gltK"
                     /locus_tag="RHZ76_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HDV2811585.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISLGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            23362..24087
                     /gene="gltL"
                     /locus_tag="RHZ76_000901"
     CDS             23362..24087
                     /gene="gltL"
                     /locus_tag="RHZ76_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HDV2811586.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            24205..25140
                     /gene="rihA"
                     /locus_tag="RHZ76_000902"
     CDS             24205..25140
                     /gene="rihA"
                     /locus_tag="RHZ76_000902"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HDV2811587.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESAEPITIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHRAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(25180..25662)
                     /locus_tag="RHZ76_000903"
     CDS             complement(25180..25662)
                     /locus_tag="RHZ76_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HDV2811588.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLMLCPKCGHDQFQRRPFEP
                     "
     gene            25897..28479
                     /gene="leuS"
                     /locus_tag="RHZ76_000904"
     CDS             25897..28479
                     /gene="leuS"
                     /locus_tag="RHZ76_000904"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDV2811589.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNNYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPQY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVATLNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            28494..29075
                     /gene="lptE"
                     /locus_tag="RHZ76_000905"
     CDS             28494..29075
                     /gene="lptE"
                     /locus_tag="RHZ76_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HDV2811590.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            29075..30106
                     /gene="holA"
                     /locus_tag="RHZ76_000906"
     CDS             29075..30106
                     /gene="holA"
                     /locus_tag="RHZ76_000906"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDV2811591.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENSAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            30108..30749
                     /gene="nadD"
                     /locus_tag="RHZ76_000907"
     CDS             30108..30749
                     /gene="nadD"
                     /locus_tag="RHZ76_000907"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDV2811592.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            30773..31384
                     /locus_tag="RHZ76_000908"
     CDS             30773..31384
                     /locus_tag="RHZ76_000908"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HDV2811593.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            31644..31961
                     /gene="rsfS"
                     /locus_tag="RHZ76_000909"
                     /gene_synonym="rsfA"
     CDS             31644..31961
                     /gene="rsfS"
                     /locus_tag="RHZ76_000909"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDV2811594.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            31965..32432
                     /gene="rlmH"
                     /locus_tag="RHZ76_000910"
     CDS             31965..32432
                     /gene="rlmH"
                     /locus_tag="RHZ76_000910"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDV2811595.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            32463..34364
                     /gene="mrdA"
                     /locus_tag="RHZ76_000911"
     CDS             32463..34364
                     /gene="mrdA"
                     /locus_tag="RHZ76_000911"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HDV2811596.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            34367..35479
                     /gene="mrdB"
                     /locus_tag="RHZ76_000912"
                     /gene_synonym="rodA"
     CDS             34367..35479
                     /gene="mrdB"
                     /locus_tag="RHZ76_000912"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HDV2811597.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            35490..36578
                     /gene="rlpA"
                     /locus_tag="RHZ76_000913"
     CDS             35490..36578
                     /gene="rlpA"
                     /locus_tag="RHZ76_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HDV2811598.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            36718..37929
                     /gene="dacA"
                     /locus_tag="RHZ76_000914"
     CDS             36718..37929
                     /gene="dacA"
                     /locus_tag="RHZ76_000914"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HDV2811599.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            38039..38302
                     /locus_tag="RHZ76_000915"
     CDS             38039..38302
                     /locus_tag="RHZ76_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HDV2811600.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            38403..39044
                     /gene="lipB"
                     /locus_tag="RHZ76_000916"
     CDS             38403..39044
                     /gene="lipB"
                     /locus_tag="RHZ76_000916"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HDV2811601.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         9607 a        10207 c        10073 g         9387 t
ORIGIN      
        1 tccggcctac aaaagcacgc aaactcaata tattgcagag atcatgtagg cctgataagc
       61 gtagcgcatc aggcaattta gcgtttgaaa ctgtttgtct gttccttgcc ggatgcgacg
      121 taaacgcccc atccggcata gcgaaactta aaattactcg cctactttcg cccaggtatc
      181 acgcagccca acggtgcggt taaataccgg tttttccgcc gtagaatggc ggctatcgag
      241 gcagaagtaa ccttcacgct caaactggaa tgctttaccc gcaaccgcat ctttcagcga
      301 cggttcagca aagccctgtt tgatcaccag cgattccggg ttaatcaccg acaggaaatc
      361 atccgcagca cctgggttag gcacgctgaa cagacggtca tacaaacgga tttcaaccgg
      421 cagcgcatgt gccgcgctca cccagtgaat aacacctttg actttacgac catctgccgg
      481 atctttgctt aaggtatcgg cgtcataagt acagaagatg gtggtgatat taccttcggc
      541 atctttctcg acgcgttctg ccttaatcac ataagcatta cgcagacgca cttctttacc
      601 cagcaccaga cgtttgtact gcttgttagc ttcttcgcgg aaatcggcgc gatcaatcca
      661 aatctcaccg ctaaacggca cctgacggct gcccatttcc ggtttgttcg gatggttcgg
      721 catggtaacc atttcgcctt cgccctgata gttttcgata accagtttca ccggatcgat
      781 aaccgccatt gcgcgcggcg cattttcgtt gagatcttca cggatgcagg attccagcga
      841 cgccatctca atggtgttgt cctgcttggt cacgccgatg cgtttgcaga actcacgaat
      901 agaagccgca gtgtaaccac gacgacgcag accggaaatg gtcggcatac gcgggtcatc
      961 ccagccttca acgtgcttgt cggtcaccag caggttcaac ttacgcttgg acatcacggt
     1021 gtattccaga ttcaggcgcg agaactcata ctggcgcggg tgaacaggaa tcgtgatgtt
     1081 gtccagtacc cagtcgtaca gacgacggtt gtcctggaac tcaagcgtac acagagagtg
     1141 cgtaatacct tccagggcat cgctgatgca gtgggtgaag tcgtacatcg ggtagatgca
     1201 ccacttgttg ccagtctggt ggtgttcagc aaatttaata cggtacagca ccggatcgcg
     1261 catcacgata aacggtgaag ccatgtcgat tttcgcacgc aggcaggctt taccttcttc
     1321 aaaaccaccg gcacgcattt tttcgaacag cgccaggttc tcttcaacgc tgcggtcgcg
     1381 gtacgggctg tttttacccg gttgcgtcag ggtgccgcgg tattcgcgga tctgttccgg
     1441 cgtcagttca tcaacgtacg ccaggccttt attgatcagt tcgatcgcat aggcgtggag
     1501 ctgatcaaaa taatcggagg agtaacggac gttaccagac cagtgaaaac ctaaccactc
     1561 tacgtcgttt ttgatcgact caacatactc gatatcttct tttaccgggt tagtgtcgtc
     1621 gaaacgcagg ttgcactggc ctttatagtc ctgggcgatc ccgaagttca ggcagataga
     1681 tttcgcatgg ccaatatgca gatagccatt cggctccggc gggaaacggg tgtgtactgt
     1741 ggtgtgctta ccactggcca gatcttcatc gatgatctga cggataaagt tagtcgggcg
     1801 ggcttctgcc tcactcatcg tggattcctc aaagcgtaaa caacgtataa cggcgtatga
     1861 tcttataagc gggacaggct gacaactgtt agttagtgaa aatcgaaaat ctcactgaga
     1921 ttatggggat gattgatgaa aaaaaaagcg gcagagaaga tctccaccgc ttttgccagt
     1981 cctgcgtcat tgttggatgc gacgcttaag cgtcttatcc aacctggact gagcttttgt
     2041 aggcctgata agacgcgtca agcgtcgcat caggcataaa gcagattact ttttgatttc
     2101 atacagcggt gtttgacccg ccacaacatg gccctgagct ttaatgatca agccactgaa
     2161 atcgtcgata ttgctgcaaa ccaccgggct aatcatcgag cgggcgttag cgttcaggta
     2221 atccagatcc atttccagaa tcggttgccc tgcgcttacc tgcgcaccct cttccaccag
     2281 acgtttaaag cctttacctt ccagcgctac ggtgtcgata cccatatgga cgacgatctc
     2341 cgcgcctttt tcggtttcca ggcagaacgc gtggttggtg ttgaagattt tcacgattgt
     2401 ccctgcggct ggtgatacga cgattttatc tgtcggtttc accgccacac cgtcacccac
     2461 cgctttgctg gcgaatgctt cgtcaggaac ctgatccagt gccacgacat caccggtaat
     2521 cggcgatacc agctccgcga tagataccgc gtttggtaca gcctgcggtt ttgctacagg
     2581 cgcggcagtt gccggagttg cttcagctga cgcagctgct accggaccgc gggcaacgac
     2641 tttcttcatc gcatcgccga tggattctgc tttcgcgcca acaatcacct gaatagtctg
     2701 tttgttcagt tttactacac cagaagcacc cagacgttta cacatcgtat cgttaacgcg
     2761 ggcagagtca gccactgtaa ggcgcagacg ggtgatacag gcgtcaattg ctttcaggtt
     2821 gtcagtgccg ccaaccgcag caatatagtt ggtcgccagt tgattgaggc cttcttcagt
     2881 gttgctgttg gcttcttcag taacgatctc gtcttcttta tcttcacgac ccggcgtttt
     2941 caggttgaac atgcggataa ccaaactgaa caccacgaag tagatagcga agaagacaac
     3001 acccatcacc agcagcatcc agacgttctg gctggcggcc ggcaggttat acatcaacgc
     3061 gtagtcgata gcccccgcag agaaagagaa gccagcatgg atacccagca gtgttgccac
     3121 aaacaggctg ataccggtca gcagtgcgtg caggaggtac agcagcggtg caaggaacat
     3181 gaacaggaat tccagcggct cagtcacacc ggtcaggaac gcagtaacag caacagaaag
     3241 cagcatcccg ccaaccatcg gacgacgctc tttcggtgct gcgaagtaca tcgccagcgc
     3301 cgcacccggc agaccgaaca tcataatcgg gaagaagccg gacatgaaca tccccgcggt
     3361 gccgtcaccg gcatagaagc ggttgatatc accgtggaaa accgtacccg ccgcgttggt
     3421 gaattcacca atctggaacc aggcgatggt gttcagcacc tgatgcagac cggttgggat
     3481 cagcagacgg ttgatgaaac caaagatacc ggaacccagc gcgcccgcag aaacgatcca
     3541 ctcgcctcct gcatggatag cgtgctgtac cggcggccag acgtaaccaa aaatggccgc
     3601 cagcaccagg cagaagaagc cggtggcaat cggcacaaag cgtttgccgc cgaagaagct
     3661 caggaagtcc ggcagtttaa tatcggacca acggttatag gctgcgccac caaccagacc
     3721 ggtaatgata cccgccagta cacccatgtt tatttctggg ttgatggtca ccatcgcttt
     3781 ggttaacaca aagtaaccta ccgcacccgc cagcgccgcc gcacctgcgc tgtctttcga
     3841 ccagctggat gccacaccga tggcgaagat taatgcgagg ttatcaaaaa tcgcaccgcc
     3901 cgcctgggca ataaacgcaa cgttaagtaa atctggctga ccgaatcgca gcaacagtgc
     3961 cgccaccggc agcaccgcga tagggagctg taacgcccta ccgagtcgct ggaaaaaacc
     4021 taaaatattc atcttattcc ccctacgaga accctatttg gctcgtttaa agccgtattt
     4081 ttattttgct gcaaattgta ctgccgatgt tctgtaatca gattgttaga tcatctgcta
     4141 cagagtgtgt gaaaatttaa ttcgtatcgc aaattaaacg cgtgtctttt gtgagttttg
     4201 tcaccaaata tcgttatcat cactcccttt tactggctaa accagaaaac ttattttatc
     4261 attcaaaaaa tcaggtcgga ttgacgcctg tctgcgcaaa tccaggttac gcttaaagat
     4321 gcctaatccg ccaacggctt acattttact tattgaggtg aataatgaga ctgatccccc
     4381 tgactaccgc tgaacaggtc ggtaaatggg ctgctcgcca tatcgtcaat cgtatcaatg
     4441 cgttcaaacc gactgccgat cgtccgtttg ttctgggcct gccaactggc ggcacgccaa
     4501 tgaccaccta taaagcgtta gtcgaaatgc ataaagcagg ccaggtcagc tttaagcacg
     4561 ttgtcacctt caacatggac gaatatgtcg gtctgccgaa agagcatccg gaaagctact
     4621 acagctttat gcaccgtaat ttcttcgatc acgttgatat tccagcagaa aacatcaacc
     4681 ttctcaacgg caacgccccg gatatcgacg ccgagtgccg ccagtatgaa gaaaaaatcc
     4741 gttcttacgg aaaaattcat ctgtttatgg gcggtgtagg taacgacggt catattgcat
     4801 ttaacgaacc ggcgtcttct ctggcttctc gtactcgtat caaaaccctg actcatgaca
     4861 ctcgcgtcgc gaactctcgt ttctttgata acgatgtcaa tcaggtgcca aaatatgccc
     4921 tgactgtcgg tgttggtaca ttgctggatg ccgaagaagt gatgattctg gtgctgggta
     4981 gccagaaagc actggcactg caggccgccg ttgaaggttg cgtgaaccac atgtggacca
     5041 tcagctgtct gcaactgcat ccaaaagcga tcatggtatg cgatgaacct tccaccatgg
     5101 agctgaaagt taagacttta agatatttca atgaattaga agcagaaaat atcaaaggtc
     5161 tgtaattgtt atccctgccc tggctccttg ctcagggcaa tattttttaa aatcgggggt
     5221 cagaatgtat gcattaaccc agggccggat ctttaccggc cacgaatttc ttgatgacca
     5281 cgcggttgtt atcgctgatg gcctgattaa aagcgtctgt ccggtagcgg aactgccgcc
     5341 agagatcgaa caacgttcac tgaacggggc cattctctcc cccggtttta tcgatgtgca
     5401 gttaaacggc tgcggcggcg tacagtttaa cgacaccgct gaagcggtca gcgtggaaac
     5461 gctggaaatc atgcagaaag ccaatgagaa atcaggctgt actaactatc tgccgacgct
     5521 tatcaccacc agcgatgagc tgatgaaaca gggcgtgcgc gttatgcgcg agtacctggc
     5581 aaaacatccg aatcaggcgt taggtctgca tctggaaggt ccgtggctga atctggtaaa
     5641 aaaaggcacc cataatccga attttgtgcg taagcctgat gccgcgctgg tcgatttcct
     5701 gtgtaagaac gccgacgtca ttactaaagt gaccctggca ccggaaatgg ttcctgcaga
     5761 agtcatcagc aaactggcaa atgccgggat tgtggtttct gccggtcact ccaacgcgac
     5821 gttgaaagaa gcgaaagccg gtttccgcgc ggggattacc tttgccaccc atctgtacaa
     5881 cgcgatgccg tatattaccg gtcgtgaacc gggcctggcg ggcgcgatcc tcgacgaagc
     5941 tgacatttat tgcggtatta tcgctgatgg cctgcatgtt gattacgcca acattcgtaa
     6001 cgctaaacgc ctgaaaggcg acaaactgtg tctggttacc gacgccaccg cgccagcagg
     6061 tgccaacatt gaacagttca tttttgcggg taaaacaata tactaccgta acggactttg
     6121 tgtggatgag aacggtacgt taagcggttc atccttaacc atgattgaag gcgtgcgtaa
     6181 tctggtcgaa cattgcggta tcgcactgga tgaagtgctg cgtatggcga cgctctatcc
     6241 ggcgcgtgcg attggcgttg agaaacgtct cggcacactc gccgcaggta aagtagccaa
     6301 cctgaccgca ttcacacctg attgtaaaat caccaagacc atcgttaacg gtaacgaggt
     6361 cgtaactcaa taagagaaag tatgacacca ggcggacaag ctcagatagg taatgttgat
     6421 ctcgtaaaac agcttaacag cgcggcggtt tatcgcctga ttgaccagta cgggccaatc
     6481 tcgcggattc agattgccga gcaaagccag cttgcccccg ccagcgtaac caaaattacg
     6541 cgtcagctta tcgaacgcgg gctgatcaaa gaagttgatc agcaggcctc caccgggggc
     6601 cgccgcgcta tctctatcgt caccgaaacc cgcaatttcc acgcaatcgg tgtacggctt
     6661 ggtcgtcacg acgccaccat tactctgttt gatctcagca gcaaagtgct ggcagaagaa
     6721 cattacccgc tgccggaacg tacccagcag acgctggaac atgccctgct gaatgccatt
     6781 gctcagttta ttgatagcta ccaacgcaag ctgcgcgagc tgatcgccat ttccgtgatc
     6841 ctgcctggac ttgttgaccc ggacagcggc aaaattcatt acatgccgca tattcaggtg
     6901 gaaaactggg ggctggtaga agcactggaa gagcgtttta aagtgacctg tttcgttggt
     6961 cacgatatcc gtagtctggc gctggcagag cactacttcg gtgcaagtca ggattgcgaa
     7021 gactccattt tagtgcgtgt ccatcgcgga accggggccg gaatcatctc taacgggcgc
     7081 atttttattg gccgcaacgg caacgtcggt gaaattggcc atattcaggt cgaaccgctg
     7141 ggtgaacgct gccactgcgg caactttggc tgcctggaaa ctatcgccgc caacgctgcc
     7201 attgaacaac gggtgttgaa tctgttaaag cagggctacc agagccgcgt gccgctggac
     7261 gactgtacca tcaaaattat ctgcaaagct gcgaacaaag gcgatagcct ggcgtcggaa
     7321 gtgattgagt atgttggtcg tcatctgggc aaaaccatcg ccattgccat caacctgttt
     7381 aacccgcaaa aaattgttat tgccggagaa atcaccgaag ccgataaagt gctgctccct
     7441 gctattgaaa gctgcattaa tacccaggcg ctgaaggcgt ttcgtactaa tctgccggtg
     7501 gtacgttctg agctggatca ccgctcggca atcggcgctt ttgcgctggt aaaacgcgcc
     7561 atgctcaacg gtattttgct ccagcatttg ctggaaaatt aatgtgcttt tatagtggcg
     7621 cttattgttg tcaatattct gggtagtcca tgaccattaa aaatgtaatt tgcgatatcg
     7681 acggcgtgct gatgcacgat aacgtcgccg taccgggtgc agcggaattt ttgcacggga
     7741 ttatggataa aggcctgccg ctggtgttgc tgaccaacta tccttcgcag actgggcaag
     7801 atctggcgaa ccgctttgcc accgcaggtg tcgatgtacc tgacagcgtg ttttatacct
     7861 ctgcgatggc gactgccgat ttcctgcgtc gccaggaagg caagaaagcg tatgtggtgg
     7921 gcgaaggcgc actgattcat gaactgtaca aagccggttt cactattacc gatgtgaacc
     7981 ctgatttcgt gattgttggc gaaacgcgtt cctacaactg ggacatgatg cataaagcag
     8041 cctatttcgt cgctaacggt gcacgtttta tcgccaccaa tccggacacc cacgggcgcg
     8101 gtttttatcc cgcgtgtggc gcgttgtgtg caggcatcga aaaaatctcc gggcgtaaac
     8161 cgttctatgt tggtaaaccg agtccgtgga tcatccgcgc ggcattaaac aaaatgcagg
     8221 cgcattcgga agaaacggtg attgtcggcg ataacctgcg taccgatatt ctggcgggct
     8281 tccaggcagg tctggaaacg attctggtgc tttctggtgt ttcatcgctc gacgatatcg
     8341 acagtatgcc gttccgccca agctggattt acccgtcggt cgctgaaatc gacgttatct
     8401 gaatagcaac cacgtctgcg gacgtggttg gctctgatga caaacgccaa actgcctgat
     8461 gcgctacgct tatcaggcct acgcagctcc tgcaatatat tgaattttca tgcttttgta
     8521 ggccggataa ggcgttcacg ccgcatccgg catgaacaaa ttgcactttg tcagcaattt
     8581 ggcccccgta aaatataacc attcaataaa acccgctaaa aattatcgat tcatcaataa
     8641 atacatcctt acaccagacc ttttttcacc aatcgacaat caccattacg ttttttattt
     8701 tttcaccgcc aaatcgcttg cgcacagtgc tgctttgcgg cattttttta aacaagcaaa
     8761 cacaacaagc aacaaatacc aggttaacgg agaaggttat gtgttcaatt tttggcgtat
     8821 tcgatatcaa aacagacgca gttgagctgc gtaagaaagc actcgagctg tcacgcctga
     8881 tgcgtcatcg tggcccggac tggtccggta tttatgccag cgataacgcc attctcgccc
     8941 acgaacgtct gtcaattgtt gacgttaacg cgggggcgca acctctctac aaccaacaaa
     9001 aaactcatgt gctggcggta aacggtgaaa tctacaacca ccaggcactg cgcgccgaat
     9061 atggcgatcg ttatcagttc cagaccggat ctgactgtga agtgatcctc gcgctgtatc
     9121 aggaaaaagg gccggaattt ctcgacgact tgcagggcat gtttgccttt gccttgtacg
     9181 acagcgaaaa agatgcctac ctgattggtc gcgaccatct ggggatcatc ccactgtata
     9241 tgggctatga cgaacacggt cagctgtatg tggcctcaga aatgaaagcc ctggtgccag
     9301 tttgccgcac gattaaagag ttcccggcgg ggagctattt gtggagccag gacggcgaaa
     9361 tccgttctta ttatcatcgc gactggttcg actacgatgc ggtgaaagat aacgtaaccg
     9421 acaaaaacga gctgcgtcag gcacttgaag attccgttaa aagccatctg atgtctgatg
     9481 tgccttacgg tgtgctgctt tctggtggtc tggattcctc aattatttcc gctatcacca
     9541 agaaatacgc agcccgtcgc gtggaagatc aggaacgctc tgaagcctgg tggccgcagt
     9601 tacactcctt tgctgtaggt ctgccgggtt caccggatct taaagcagcc caggaagtgg
     9661 caaaccatct gggcacggtg catcacgaaa ttcacttcac tgtacaggaa ggtctggatg
     9721 ccatccgcga cgtgatttac cacatcgaaa cttatgatgt gaccacaatt cgcgcttcaa
     9781 caccgatgta tttaatgtcg cgtaagatca aggcgatggg cattaaaatg gtgctgtccg
     9841 gtgaaggttc tgatgaagtg tttggcggtt atctttactt ccataaagcg cccaacgcta
     9901 aagaactgca tgaagagacg gtgcgtaaac tgctggccct gcatatgtat gactgcgcgc
     9961 gcgccaacaa agcgatgtca gcctggggcg tggaagcacg cgttccgttc ctcgacaaaa
    10021 aattccttga tgtggcgatg cgcattaacc cgcaggataa aatgtgcggt aacggcaaaa
    10081 tggaaaaaca catcctgcgt gaatgttttg agtcatacct gcccgcaagc gtggcctggc
    10141 ggcagaaaga gcagttctcc gatggcgtcg gttacagttg gatcgacacc ctgaaagaag
    10201 tggcggcgca gcaggtttct gatcagcaac tggaaactgc ccgcttccgc ttcccgtaca
    10261 acacgccaac ctcaaaagaa gcgtatctgt accgggagat ctttgaagaa ctgttcccgc
    10321 ttccgagcgc cgctgagtgc gtgcctggcg gtccttccgt cgcgtgttct tccgctaaag
    10381 cgatcgaatg ggatgaagcg ttcaagaaaa tggacgatcc atctggtcgt gcggttggtg
    10441 ttcaccagtc ggcatataag taagaaatgc gaaacagccc cggtgaaatc cggggctgtt
    10501 tcagttattg aggtgcttgt tgattctgat atgcagtaag gaaatgttcc gggtttgcca
    10561 catcactgtt tgacaactca tcactgtagg ccagtatttc atagattaat ttcccaccac
    10621 tattgttgaa ttccgaattt catcctgtac gacaaaagaa tggctgtagg gtggggaaaa
    10681 aacacaaagt aaacaataat tgacgaatat agcgccacgc tgttcgcaac ctaaccaaac
    10741 agtcactttc tagcaatttt ccttgaaaaa gaggttgacg ctgcaaggct ctatacgcat
    10801 aatgcgcccc gcaacgccga taaggtatcg cgaaaaaaaa gatggctacg tagctcagtt
    10861 ggttagagca catcactcat aatgatgggg tcacaggttc gaatcccgtc gtagccacca
    10921 tcttttttgc gggagtggcg aaattggtag acgcaccaga tttaggttct ggcgccgcaa
    10981 ggtgtgcgag ttcaagtctc gcctcccgca ccattcacca gaaagcgttg tacggatggg
    11041 gtatcgccaa gcggtaaggc accggttttt gataccggca ttccctggtt cgaatccagg
    11101 taccccagcc atcgaagaaa caatctggct acgtagctca gttggttaga gcacatcact
    11161 cataatgatg gggtcacagg ttcgaatccc gtcgtagcca ccaaattctg aatgtgtcga
    11221 atatgttcgg caaattcaaa accaatttgt tggggtatcg ccaagcggta aggcaccgga
    11281 ttctgattcc ggcattccga ggttcgaatc ctcgtacccc agccacatta aaaaagctcg
    11341 cttcggcgag ctttttgctt ttctgcgttc attcaatgtc gaatgcgatg ttgacacgtc
    11401 ttatccttca atgtcggatg cgacgctgcc gcgtcttatc cgacctacgg ttggcacgca
    11461 tccggcaatg ttgtaaggct acaaccctaa cgcatatttc agcgcctgac gtttcaacac
    11521 gccagcacgc tccgccgcca ttaacccgag attacgcata aaacgcagtg gtggcagatt
    11581 attgctgaat ccggcataaa acagatccat accgctttgc ataatgaagt tatccgccat
    11641 gcgccgcatc tggtaacgct tgaggacagg ataactggcc cacgcttcgc cgtagctgcg
    11701 ggcgttgacc agaacatcaa tcagggcatc gacatcacga taaccaagat tcactccctg
    11761 ccccgccagc ggatggatgg tatgcgcggc atcgcccacc agcgcaagcc ctggctgcac
    11821 gtactgcaac gcatggcgac gcgtcagcgg aaacgcacca gcggcaagcg gtgtaacgta
    11881 acccagacgc gacgggaaat gcttcgcgat ttccgcctgg agctgtgcca tattcatatt
    11941 ctgcaactgg cgaatacgcg ccggagagtc ataccacacc agcgatgccc agttatcaaa
    12001 caacggcaga aacgcacgcg gtccgtccgg agtaaattgc tgccaggtgc tgtcgccggg
    12061 atcgttctcg cactggacgc taatcaacat gcacgactgc gcatactgcc atgcatgaac
    12121 gccaattccc gccatctgcc gcacctgcga atttgcgccg tcggcaccaa tcaccagctt
    12181 cgcgcgaatc acttcaccgc ctttcagctc cagctcctga agatcatcat ggcgatgcag
    12241 cgcaatcagc gagcctggca cacgtaacgt tactttcgga tgcgcttcca gcgcctgcca
    12301 caacgcctgt tgcaggacag tgttttccac catatagcca agcagcggta gcttaagttc
    12361 agcggcgtca aacaccacat gcgccgtttc ccactcccac gtttccagtc tgcggtaagg
    12421 atggcaacgc atagcctgta ctgcatccca gacccctaac cctttaagca atgataccga
    12481 agccgcgctg atcgccgaga tccgcacgtc cggttggcta tcagcgacaa acggcgctgg
    12541 ttctgcgtgc tcgatcaccg ttaccgcaaa tccgtgctgt gccagcccca gcgccagtgc
    12601 gccgccgacc attcctccgc cgacaatggc aatttccgtt ggttgatttg tcatggtcaa
    12661 tcatcctgtt agcaatatgc tttaagttta caggattttt acccctaagg gctgataacg
    12721 ctcatactgg tcacaacggc agcaaagcat tacactatgc gccctgcatt cctggctact
    12781 atttcgcaag agcaagtcga tgaccaaaaa actccatatt aaaacctggg gctgtcagat
    12841 gaacgagtac gattcatcga agatggccga tctgctggat gccacccacg gctatcaact
    12901 gacagacgtg gcggaagaag cggatgtgct gctgctgaac acctgctcaa tccgcgagaa
    12961 ggctcaggaa aaagtcttcc atcagttggg ccgctggaaa ctgttaaaag agaagaatcc
    13021 agacctgatt atcggcgtcg gtggctgcgt ggcatcgcaa gaaggcgagc acattcgcca
    13081 gcgcgcccac tatgtcgata ttatttttgg gccgcaaacg ctgcaccgtc tgccggagat
    13141 gatcaactcc gtgcgtggcg accgcagccc ggttgtagat atcagcttcc cggaaatcga
    13201 gaagtttgac cgtctaccgg aaccgcgcgc cgaagggccg accgcgtttg tctccatcat
    13261 ggaaggctgc aataaatatt gcacctactg cgtggtgcct tacacccgtg gtgaagaggt
    13321 aagccgtccg tccgacgata ttctgtttga gattgcccag cttgcggctc agggcgtgcg
    13381 tgaagtcaac ctgctcggtc agaacgtgaa cgcctggcgt ggtgaaaact acgacggcac
    13441 caccggatcg tttgccgatc tgttgcgtct ggttgctgcg atcgacggga tcgatcgtat
    13501 tcgctttacc accagccatc cgatcgaatt caccgacgat atcatcgaag tgtaccgcga
    13561 cacgccggag ctggtgagct tcctgcatct gccggtacag agcggttccg atcgcattct
    13621 gaacctgatg ggccgtactc atacggcact ggagtacaaa gcgatcatcc gtaaactgcg
    13681 tgcggcgcgt ccagatattc agatcagttc tgatttcatc gttggcttcc ctggcgaaac
    13741 caccgaagac ttcgagaaaa cgatgaagct gattgccgac gtcaatttcg acatgagcta
    13801 cagctttatc ttctctgcac gtccgggtac accagccgcc gatatggttg atgatgttcc
    13861 ggaagaagag aagaagcaac gtctgtatat tctgcaagaa cgtatcaatc agcaagcgat
    13921 ggcatggagc cgccggatgc tcggcaccac ccagcgtatt ctggtagaag ggacttcgcg
    13981 taagagcatc atggagcttt ccgggcgtac cgaaaataac cgcgtggtca acttcgaggg
    14041 cacaccggat atgatcggta aattcgtcga tgtagaaatt accgacgtct acccgaactc
    14101 tctgcgcggt aaagtggtgc gtactgaaga tgaaatgggt ctgcgcgtgg cagaaacacc
    14161 ggaatcggtg attgcccgta cccgcaaaga aaacgacctt ggcgtgggtt attatcagcc
    14221 gtaattctca ggcccgccgt tccggtgggc cttgtttttt ctctctttat ccccatcttt
    14281 atcgcaatgc ttgcgccttt ttcctgtggc gtgaataatt tccttatagg ccggtgaatg
    14341 ttcagcttcc ggcggcatac agataagagg aacggtttga acatagacac tcgcgaaatc
    14401 accctggagc cagcagacaa cgcgcgtctg ttgagcctgt gcggcccgtt tgatgacaac
    14461 atcaagcagc tcgaacgccg tctcggcatc gagatcaatc gccgcgacaa ccactttaaa
    14521 ctgaccggcc gtccgatttg cgtcaccgct gcggcagaca ttctgcgtag cctgtatgtc
    14581 gatacagccc cgatgcgcgg tcagattcag gatatcgaac cggaacagat ccaccttgcg
    14641 attaaagaag caagggtact ggagcaaagc gcggagagcg tgccggagta cggcaaagcg
    14701 gtcaatatca aaaccaaacg cggcgtaatt aagccgcgca cgccaaacca ggcgcagtac
    14761 atcgccaata ttctcgacca tgacattacc ttcggcgttg gcccggcggg tacgggtaaa
    14821 acctacctgg cagtcgctgc agcagttgat gccctggagc gtcaggaaat tcgccgtatt
    14881 ctgctgactc gtccggcggt cgaagccggt gagaaactgg gcttcctgcc tggcgattta
    14941 agccagaaag tagacccgta tctgcgccca ctgtacgacg cgctgtttga aatgctgggc
    15001 tttgagaaag tcgagaaact gattgagcgc aacgttattg aagtcgcgcc gctggcctat
    15061 atgcgtggtc gtacgctgaa tgacgccttt atcattctcg atgagagcca gaacaccacc
    15121 atcgaacaga tgaagatgtt cctgacccgt atcggtttta actcaaaagc ggttatcacc
    15181 ggcgacgtca cacagatcga cctgccgcgt aatactaaat caggcttacg tcatgccatc
    15241 gaagtgctgg ccgatgtcga agagatcagc tttaacttct tccacagcga agacgtggtt
    15301 cgtcacccgg tggtggcgcg tatcgttaac gcctatgaag cctgggaaga agccgaacaa
    15361 aaacgtaaag cggcgctggc ggcagaacgc aagcgcgaag aacaggaaca aaaatgagtc
    15421 aggtgatcct cgatttacaa ctggcatgtg aagataattc cgggttaccg gaagagagcc
    15481 agtttcagac atggctgaat gcggtgatcc cgcagtttca ggaagagtcg gaagtgacta
    15541 ttcgcgtggt cgataccgcc gaaagccaca gtctgaatct gacctatcgc ggtaaggata
    15601 agccgaccaa cgtgctctcc ttcccgtttg aagtgccgcc tggcatggag atgtcgctac
    15661 tgggcgatct ggttatctgc cgtcaggtgg ttgagaagga agctcaggag caaggcaaac
    15721 cactggaggc gcactgggcg catatggtgg tgcacggcag tctgcatttg ttaggttacg
    15781 atcacatcga agatgacgaa gcagaagaaa tggaagccct cgaaacagag attatgcttg
    15841 ctctgggcta tgaggatccg tacattgccg agaaagaata atttgccagg catttgactg
    15901 gcagacgtcc ccgccgttgg tgattaacga cggcgttttg attaactaac atgagaaccc
    15961 atacgacgcc atgagcgacg acaattcaca cagtagtgac acgataagca acaagaaggg
    16021 atttttctcc ctgttactca gccaactttt ccacggtgaa ccgaaaaacc gtgacgaact
    16081 gctggcgctg atccgtgatt ccgggcagaa cgaccttatc gacgaagata cgcgcgatat
    16141 gctcgaaggg gtgatggaca tcgcagacca acgcgttcgc gacatcatga tcccccgctc
    16201 ccagatgatt accctgaaac gcaaccagac gctggacgaa tgccttgatg tcatcatcga
    16261 gtccgcccac tcacgtttcc cggtgattag cgaagacaaa gatcacattg aagggattct
    16321 gatggcgaaa gacttgctgc cgtttatgcg cagcgatgct gaagccttca gcatggacaa
    16381 agtgttacgt caggcggttg tcgttcctga aagtaagcgc gtagaccgga tgctgaaaga
    16441 gtttcgctct cagcgttacc acatggcgat cgttattgac gaattcggtg gggtttccgg
    16501 tctggtgacc attgaagaca tcctggaact gattgttggt gagattgaag acgagtatga
    16561 cgaagaagat gatatcgact tccgtcagct gagtcgtcat acctggaccg tgcgcgcact
    16621 ggcttccatt gaagacttca acgaagcgtt cggcacccac tttagcgatg aagaagtcga
    16681 cactatcggt ggtctggtga tgcaggcgtt tgggcatctt ccggcgcgtg gcgaaactat
    16741 cgacatcgac ggttaccagt tcaaagtggc gatggccgat agtcggcgta ttattcaggt
    16801 tcatgtcaaa atcccggatg actcacccca gccaaagctg gatgaataaa accgaaactg
    16861 gatagataac tacatggctt ttgcctcatt aattgaacgc cagcgcattc gcctgctgct
    16921 ggcgttatta ttcggtgcct gcggaacgct ggccttctct ccttacgacg tctggcctgc
    16981 ggcgattatt tcgctgatgg ggcttcaggc gttgaccttt aaccgtcgcc cactccagtc
    17041 tgccgctatt ggcttttgct ggggatttgg cctctttggc agcggtatta actgggtcta
    17101 tgtcagcatc gcgacctttg gcgggatgcc tggcccggtt aacatcttcc tggtggtgct
    17161 gctggcggcg tatttgtcgc tgtataccgg actgtttgct ggcgtgctgt cgcgtctgtg
    17221 gccgaaaacc acctggctgc gcgtggcgat tgctgcccct gccctctggc aagtgaccga
    17281 gtttctgcgc ggttgggtac tgaccggctt cccgtggtta cagttcggct atagccagat
    17341 tgatggcccg ttaaaagggc tggcaccgat aatgggcgtg gaagccatta acttcctgct
    17401 gatgatggtt agtggcctgc tggcactggc gttggtcaaa cgcaactggc gtccgctggt
    17461 ggtggccgtc gtgctgtttg ccctaccctt cccgctgcgt tacatccaat ggtttacccc
    17521 gcaaccggag aaaaccattc aggtttcgat ggttcagggc gatatcccac aatcgctgaa
    17581 atgggacgaa ggccagcttc ttaatacgct gaagatttac tacaacgcaa cggcaccgct
    17641 gatgggcaaa tcatcgttga ttatctggcc ggagtcggcg ataaccgatc tggaaattaa
    17701 tcagcaaccg ttcctcaaag cactggacgg tgagttgcgt gataaaggta gctcgctggt
    17761 caccgggatt gtcgacgcgc gtctcaataa gcagaaccgc tacgatacct acaacaccat
    17821 catcacgctg ggtaaaggtg caccgtacag ctacgaatca gccgatcgct ataacaaaaa
    17881 ccatctggtg ccgtttggcg agtttgtccc gctggagtcg attctgcgtc cgttagcacc
    17941 gttctttgat ctgccgatgt cgtcgttcag ccgtgggcca tatatccagc cgccgctgtc
    18001 ggtaaatggt attgagctta ctgcggctat ttgctacgag atcattctcg gcgagcaagt
    18061 gcgcgataac ttccgcccgg ataccgacta cctgctgact atctccaacg atgcatggtt
    18121 tggtaagtct attggtccat ggcagcactt ccagatggcg cgaatgcgtg cgctggagct
    18181 ggcgcgccca ttgctgcgta gcaccaacaa cggcattacg gcggtgattg gcccgcaggg
    18241 tgagattcag gcgatgatcc cgcagttcac ccgcgaggtg ttgaccacta acgtgacgcc
    18301 gacgaccgga ctcacaccgt acgcacgaac cggcaactgg ccgctgtggg tgctgacggc
    18361 gctgtttggt tttgccgctg tgctgatgag tctgcgagca cgtaaacgtt gatgtgcctg
    18421 atgcgctacg cttatcaggc ctacgcgatt tttgcaatat gttgaattta cgtgattttg
    18481 taggataaag cgtactttct caacacgctg tggggcatga ttatcatgcc cctttttctt
    18541 acggttcagg gatacaagcc acattgccat attggggatg acgaataact gcctgcggat
    18601 caagcgagag cgtcctgtcc atttggatta acccgttctt cgccataaca aatatctttc
    18661 taccatccgc cgaaaaacga aggtgattaa cgtagctggc aggataaacg ccaacaatct
    18721 gcccgacatt aatattgagc aggatgacat aaccttgcaa gccatatgac accaacgcta
    18781 aatactgacc gtccgggcta ataccctcaa cggaataaac gctcctgcta caccattgcg
    18841 tcataatgct ggcttgctga tccccaactc ttatacgtga aatcccctgg ctgtcggcaa
    18901 aaattacatc cccgttttca ggtcgaaaac gcatcgccga tatcgttcca cgcgcatcac
    18961 ctgcgggaaa gtcttctttc tgatgcgtga tggtattgat gcgtacaatg tcacctctga
    19021 ccataccggg tatcaacaat gttttttcat ccggggaaag tgccataacc caggcaccag
    19081 gtttaccgct cgtccccgtc ccgtcgtatt tctccagcgg gatatccgcc acatgctgat
    19141 aagtcaccag atcaatcgct gacacactat tactggtgcg attcatcacc caggcttttt
    19201 tgccatcacg cgagagtata aggctctcag gatgtgcacc ggtggtaatc gactgaataa
    19261 tctcccccgt cgccacgtta atgaccgata acgcattcgc ttcaaatgaa gtcgcataaa
    19321 gcaacttttc atccgggctt aacgccagcg atctgacgcc ctgatctttc cagccaccac
    19381 aatgtttgac tgacttttct ttcgctatgg gcgctgtaaa ggtatgcagg attttctttt
    19441 gtccgaggtc gaacacaaca atgctgtttg tgttttcaat agcggcatat aacgtattac
    19501 catcccgact gatcgccata tcatggatat cggcaatctc agcgagcaaa tcaccactgg
    19561 cctgttgacg acttaccgga gcgggtaatg tttctttgag ggcgttgcgt tcctccgcta
    19621 actgttgctt ttttgtcgtc gctcttgttc aatctcctgc ggataaaacg cttcacgcag
    19681 gctttgcctg acctgtaaaa ctgattcatc aatctgctgc tgcgcaagcc agatagcccc
    19741 ggtaagaaga agactgcccg caacaataat cccgccttcc gtaacaagcc ggttttgccc
    19801 agataaacgc gcactcagcc aacccaacgc gccacccgca atcaggccgc caatgtgaca
    19861 agcattatct attcctgttt gccgggcacc atacaacagt gtcagcgcca ccatacctaa
    19921 cagagaaaat actcgccgct gatttttatg gtgcgtgccc gtacctgaag caagctgtgt
    19981 tgctatcgca gcgccagcta ttcccataat tgctccggat gccccaacgc tgatgactat
    20041 ctgctcacta tcgcgtaacg cataacaggc gctgatgagc ccaccaaaga cgccggagaa
    20101 taaccagata atcaggagtc gccatttccc cagtatccgt tccgccactg cccccaccac
    20161 caggaatgca aacatattca tcagcagatg agaaacactg ctgtgcagaa agacactgct
    20221 caataaacgc cagggttcac ccgacaacgt gagagaagca acgttagcgc cgagatggat
    20281 aagtgcggat tgatcgactt cttcataact catccattgt gcgaggtaac aaaatatccc
    20341 aacacagagc gcaaaaagga taagggttat tgcgggtaat tgaacattta acggcttaac
    20401 cgaagatgca gacataagat gattcttcct tgaaaagaca ggtaaaaagc aattttcccg
    20461 tgtgcagtta tcatcccggc aaacgtccca taccgaaaag ttaaccctgt ccgaaatatg
    20521 actttttcag cctgaaatta caatcgtaat taattcactt ctaagtattt tttggcatgt
    20581 aagttgcttt atttactgtg taagcaatga aatcaaccgc gacgcaaccg ggttgcacca
    20641 ccacaaatca gcggtagcac caaaaaggtg cggtttaaga attttgcttc ggaatggtgc
    20701 ggcgcatctc gcaaaaacaa acaataaaac agcaatatct ttacatttag ccaaactaaa
    20761 tgttacctaa caggcacaac actgcacaat aaagttgcag acgataacaa cacaaacact
    20821 cacaacgggt atccatgcgt tcttaacgca gaagataaag gagttggata tgcaattacg
    20881 taaacctgcc acagcaatcc tcgccctggc gctttccgca ggactggcac aggcagatga
    20941 cgccgccccg gtagcgggca gtacgctgga caaaatcgcc aaaaacggcg tgattgtcgt
    21001 cggtcaccgt gaatcttcag tacctttctc ttattacgac aatcagcaaa aagtggtggg
    21061 ttactcgcag gattactcca acgccattgt tgaagcagtg aaaaagaaac tcaacaaacc
    21121 ggacttgcag gtaaaactga ttccgattac ctcacaaaac cgtattccac tgctgcaaaa
    21181 cggcactttc gattttgaat gtggttctac caccaacaac gtcgaacgcc aaaaacaggc
    21241 ggctttctct gatactattt tcgtggtcgg tacgcgcctg ctgaccaaaa agggcggcga
    21301 tatcaaagat tttgccgacc tgaaaggcaa agccgtagtc gtcacttccg gcactacttc
    21361 tgaagttttg ctcaacaaac tgaatgaaga gcaaaaaatg aatatgcgca tcattagcgc
    21421 caaagatcac ggtgactctt tccgtaccct ggaaagcggt cgtgccgttg cctttatgat
    21481 ggatgacgct ctgctggccg gtgaacgtgc gaaagcgaag aaaccagaca actgggatat
    21541 cgtcggcaag ccgcagtctc aggaagccta cggttgcatg ttgcgtaaag atgatccgca
    21601 gttcaaaaag ctgatggatg acactatcgc tcaggtgcag acttccggtg aagcggaaaa
    21661 atggtttgat aaatggttca aaaatccaat tccgccgaaa aacctgaaca tgaatttcga
    21721 actgtcagac gaaatgaaag cactgttcaa agaaccgaat gacaaagcac tgaactaatt
    21781 acaagaacca ggggcggaaa ttctagccct ctcgattgtt acgtagcacg gacagactat
    21841 acgcctgatg gtcgttcccc atcgggcctg aaaaccgcaa tacgctgggt aacaatcttc
    21901 gagggtagca gttaacgctg ctaccctctt ttttctggag tagatttatg tctatagact
    21961 ggaactgggg tattttttta caacaagccc cgttcggcaa caccacctat cttggttgga
    22021 tctggagtgg ttttcaggtg acgatcgctt tgtcgatctg cgcctggatt atcgctttcc
    22081 tcgtcggttc atttttcggc attttacgta ccgttccaaa ccgttttctc tccggtctgg
    22141 gtacgttgta tgtcgaactg ttccgcaacg tgccgttgat tgtgcaattc tttacctggt
    22201 atctggtgat cccggagctg ctgccggaga aaatcggcat gtggtttaag gccgagctgg
    22261 atcccaatat tcagtttttc ctttcatcca tgctctgcct ggggctgttt accgccgccc
    22321 gtgtttgcga acaggtacgc gccgctattc agtcgctgcc gcgtgggcag aagaatgccg
    22381 cgctggcgat gggtctgacg ctaccgcagg cttaccgcta cgtgctgttg cctaacgcgt
    22441 atcgcgttat cgtcccgcca atgacttccg agatgatgaa cctggtgaaa aactccgcca
    22501 tcgcctcgac tatcggtctg gtggatatgg cagcgcaggc gggtaaattg ctcgactact
    22561 cggcccacgc ctgggagtcg tttaccgcca ttacgcttgc gtatgtgttg attaacgctt
    22621 ttatcatgct ggtgatgacg ctggttgaac gtaaagttcg cctgcccggc aacatggggg
    22681 gcaaataatg tacgagtttg actggagttc cattgttcct tccctgccat atctgctcga
    22741 cgggctggtg atcaccctga aaatcaccgt cacggcagtc gtgattggta ttttgtgggg
    22801 taccatgctg gcggtgatgc gcttgtccag cttcgcgcct gtttcgtggt ttgccaaagc
    22861 ctacgttaac gtattccgct cgattccttt agtcatggtt ttgctgtggt tttacttgat
    22921 tgtgccggga tttctgcaaa atgtgctggg attatcgcca aaaaatgata ttcgtttaat
    22981 ttcggcgatg gtggcgtttt cgatgtttga agcggcctac tattcagaga ttatccgtgc
    23041 cggtattcaa agtatttctc ttggtcagtc gagcgccgcg ctggcgttgg gaatgactca
    23101 ctggcagtcg atgaaactga ttattctgcc gcaggcgttc cgcgcgatgg tgccgctgct
    23161 gctcactcag ggcatcgtac tgttccagga tacctcgctg gtgtatgtgt tatctctggc
    23221 cgatttcttc cgtaccgcct caaccattgg tgagcgtgat ggtacgcagg ttgagatgat
    23281 cctgtttgcc ggatttgttt atttcgttat tagtcttagc gcgtcgttgt tggtcagcta
    23341 cttgaaaaga aggacagcat aatgattacc ctgaaaaatg tttcaaaatg gtatggtcac
    23401 tttcaggtgc tgaccgactg ctcaaccgaa gtgaaaaaag gcgaagtggt ggtggtttgc
    23461 ggcccgtctg gttccggcaa atcaacgctg attaaaaccg tcaacggcct cgaaccagtg
    23521 cagcaaggtg aaatcaccgt cgatggtatc gtggttaatg acaagaaaac cgatctggca
    23581 aagctgcgtt cccgcgtcgg gatggtattc caacatttcg agctgttccc tcatctgtcg
    23641 attatcgaaa acctgaccct ggcgcaggtg aaagtgctta aacgcgataa agcaccggct
    23701 cgtgaaaaag cattgaaatt gctggagcgc gtgggacttt ccgcacatgc caataaattt
    23761 ccggcgcagc tttccggcgg ccagcagcag cgtgtggcga tcgctcgcgc attgtgtatg
    23821 gatcctattg ccatgctgtt cgacgaaccg acatcggcgc tggatccgga gatgatcaac
    23881 gaagtgctgg acgtaatggt cgaactggcg aacgaaggaa tgaccatgat ggtggtgacc
    23941 cacgaaatgg gctttgcccg taaagtggcg aatcgggtga tctttatgga cgagggtaaa
    24001 attgtcgaag actcgccgaa agacgctttc ttcgatgatc cgaaatcgga ccgcgcaaaa
    24061 gatttcctcg cgaaaatcct gcattaatca ctctggcgcg catacccaat tgcgcgccat
    24121 ctcacgcttt atcgtttcgc ccctcgtatc tgttacatcc tggcgttacc cttgtcgcaa
    24181 agaagcacac aacaaggagc aacaatggca ctgccaattc tgttagattg cgacccaggt
    24241 catgacgacg ctatcgcaat agttctcgcc ctcgcctcac cagagcttga tgtcaaagca
    24301 attacgtctt ccgccggaaa ccagacacca gaaaaaacct tacgcaatgt tctgcgtatg
    24361 ctgaccttgc ttaatcgcac cgatattccg gtagcaggcg gcgcggtaaa accgttaatg
    24421 cgtgagttga ttatcgcgga caatgtgcac ggcgaaagcg gtctcgacgg cccggcatta
    24481 ccggaaccgg cgttcgcacc gcaaaactgt acggcggtag agctaatggc gaaaacgctg
    24541 cgtgaaagtg cggaacctat cacgattgtg tctaccggac cgcaaactaa cgttgccttg
    24601 ctgctcaata gccatccgga actgcatagc aaaattgccc gtatcgtgat tatgggcggc
    24661 gcaatggggc tgggtaactg gacgcctgcg gctgaattta atatttacgt tgacccggaa
    24721 gcggcagaaa ttgtcttcca gtcagggatc ccggtggtga tggccggtct ggatgttact
    24781 cacagagcac aaatccacgt tgaagacact gagcgtttcc gcgcgattgg caaccctgtt
    24841 tcaaccattg ttgccgaact gctggatttc ttcctcgaat atcataaaga cgaaaaatgg
    24901 ggctttgtcg gcgcaccgct gcatgaccca tgcaccatcg cctggctgtt gaaaccggag
    24961 ttgtttacca ctgttgagcg ctgggttggc gtggaaacac aagggaaata tacccagggt
    25021 atgacggttg ttgattatta ttatctgact ggcaataaac cgaatgcaac tgtaatggtc
    25081 gatgttgatc gtcagggctt tgttgattta ctggctgatc gtctgaaatt ttacgcttaa
    25141 caactcgccg gatgacatta cgtcatccgg caccattcat tacggctcga acgggcgtct
    25201 ctggaactga tcatgaccac atttcgggca tagcatcagc acttccggtg tgtagatcgg
    25261 gagatggaag tgacatttct cgcagaccag atttcccagc cctaccactt ctccgctgtg
    25321 ataaaccccg tgatgattga ggtcctggaa aacttcgcgc cattcaagct gcgttttatc
    25381 ggtgatgtct gccagctcct gccacaagct ttctttaatc acccgcataa agacgctgtc
    25441 agattcttct ttcaggctct cttcatagct catggcgaac tcttccaggt cacgtctgac
    25501 agctcgcgtc agctcatcga cctcggttcg cgttaactcc cctgttttta ttacgcgctc
    25561 gcgcgcctgt tccaccagtg cgtcgatatc acgttcgcca ttacgcaggc gttcgctcag
    25621 tgacgcgacc agttcacggt aatattgagc aaccttgttc atcgtttcat ctcccgggtc
    25681 gttaactgac tataataata gacgttcttt gttccttgct ttgctaatac gcccacaaac
    25741 tctgtaaatt aatgtatgaa taatccgggc atcatttcaa cgtcgagtgc gcgaaaggct
    25801 gttttgacgc ggacgtttgg gctatgctat gcggatctga aaaaccacct caacgctaca
    25861 tttgtagccg tattgaaaac aggaccactg gctgccatgc aagagcaata ccgcccggaa
    25921 gagatagaat ccaaagtaca gcttcattgg gatgagaagc gcacatttga agtaaccgaa
    25981 gacgagagca aagagaagta ttactgcctg tctatgcttc cctatccttc tggtcgacta
    26041 cacatgggcc acgtacgtaa ctacaccatc ggtgacgtga tcgcccgcta ccagcgtatg
    26101 ctgggcaaaa acgtcctgca gccgatcggc tgggacgcgt ttggtctgcc tgcggaaggc
    26161 gcggcggtga aaaacaacac cgcgccagca ccgtggacgt acgacaacat cgcgtatatg
    26221 aaaaaccagc tcaaaatgct gggctttggt tatgactgga gccgcgagct ggcaacctgt
    26281 acgccggaat actaccgttg ggaacagaaa ttcttcaccg agctgtataa aaaaggcctg
    26341 gtatataaga agacttctgc ggtcaactgg tgcccgaacg accagaccgt actggcgaac
    26401 gaacaagtta tcgacggctg ctgctggcgc tgcgatacca aagttgaacg taaagagatc
    26461 ccgcagtggt ttatcaaaat cactgcttat gctgacgagc tgctcaacga tctggataaa
    26521 ctggatcact ggccagacac cgttaaaacc atgcagcgta actggatcgg tcgttccgaa
    26581 ggcgtggaaa tcaccttcaa cgttaacaac tatgacaaca cgctgaccgt ttacactacc
    26641 cgcccggaca cctttatggg ttgtacctac ctggcggtag cggcgggtca tccgctggcg
    26701 cagaaagcgg cagaaaataa tcctgaactg gcagccttta ttgacgaatg ccgtaatacc
    26761 aaagttgccg aagctgaaat ggcgacgatg gagaaaaaag gcgtcgatac tggctttaaa
    26821 gcggttcacc cattaacggg cgaagaaatt cccgtttggg cagcaaactt cgtattgatg
    26881 gagtacggca cgggcgcagt tatggcggtt ccgggtcacg accagcgcga ctacgagttt
    26941 gcctctaaat atggcctgaa catcaagcca gttattctgg cggctgacgg ttctgaaccg
    27001 gatctctccc agcaagccct gactgaaaaa ggcgtgctgt tcaactctgg cgagttcaac
    27061 ggtcttgacc atgaagcggc cttcaacgcc atcgccgata aactgactgc gatgggcgtt
    27121 ggcgagcgta aagtgaacta ccgcctgcgc gactggggtg tttctcgtca gcgttactgg
    27181 ggcgcgccga ttccgatggt gacgctggaa gacggtaccg taatgccgac cccggacgac
    27241 cagctgccgg tgatcctgcc ggaagatgtg gtgatggacg gcattaccag cccgattaaa
    27301 gcagatccgg agtgggcaaa aactaccgtt aacggtatgc cagcgctgcg tgaaaccgac
    27361 actttcgaca cctttatgga gtcctcctgg tactatgcgc gctacacttg cccgcagtac
    27421 aaagaaggta tgctggattc taaagcggct aactactggc tgccggtgga tatctacatt
    27481 ggtggtatcg aacacgccat tatgcacctg ctctacttcc gcttcttcca caaactgatg
    27541 cgtgatgcag gcatggtgaa ctctgacgaa ccagcgaaac agttgctgtg tcagggtatg
    27601 gtgctggcag atgccttcta ctatgttggc gaaaacggcg aacgtaactg ggtttccccg
    27661 gttgatgcta tcgttgaacg tgacgagaaa ggccgtatcg tgaaagcgaa agatgcggca
    27721 ggccatgaac tggtttatac cggcatgagc aaaatgtcca agtcgaagaa caacggtatc
    27781 gacccgcagg tgatggttga acgttacggc gcggacaccg ttcgtctgtt tatgatgttt
    27841 gcttctccgg ctgatatgac tctcgaatgg caggaatccg gcgtagaagg ggctaaccgc
    27901 ttcctgaaac gtgtctggaa actggtgtac gagcatacag caaaaggtga tgttgcgaca
    27961 ctgaacgttg atgcactgac tgaagatcag aaagcgctgc gtcgtgatgt gcataaaact
    28021 atcgctaaag tgaccgatga tatcggccgt cgtcagacct tcaacaccgc aattgcggcg
    28081 attatggagt tgatgaacaa actggcgaaa gcaccgaccg atggcgagca ggatcgcgct
    28141 ctgatgcagg aagctctgct ggccgttgtc cgtatgctta acccgttcac cccgcacatc
    28201 tgcttcacgc tgtggcagga actgaaaggc gaaggcgata tcgacaacgc gccgtggccg
    28261 gttgctgacg aaaaagcgat ggtggaagac tccacgctgg tcgtggtgca ggttaacggt
    28321 aaagtccgtg ccaaaatcac cgttccggtg gacgcaacgg aagaacaggt tcgcgaacgt
    28381 gctggccagg aacatctggt agcaaaatat cttgatggcg ttactgtacg taaagtgatt
    28441 tacgtaccag gtaaactcct caatctggtc gttggctaag cgcgggagga agcgtgcgat
    28501 atctggcaac attgttgtta tctctggcgg tgttaatcac cgccgggtgt ggctggcatc
    28561 tgcgtgatac cacgcaggtt ccttccacta tgaaggtcat gatcctggac tcaggcgatc
    28621 cgaacgggcc attaagccgt gcggtgcgta accagttacg tctgaatggt gtcgagttgc
    28681 ttgataaaga aaccacgcgt aaggacgttc catccttgcg tttgggtaaa gtgagcatcg
    28741 cgaaagatac cgcatcggta ttccgtaacg gtcaaacagc agagtatcag atgatcatga
    28801 cggttaatgc gaccgtgttg atccccggcc gtgatatcta cccgattagc gccaaagtct
    28861 tccgttcgtt cttcgataac ccgcaaatgg cgttagcgaa agataacgaa caagacatga
    28921 tcgtaaaaga gatgtacgac cgtgctgccg aacagctgat tcgtaagctg ccaagcatcc
    28981 gtgctgcgga tattcgttcc gacgaagaac agacgtcgac cacaacggat actccggcaa
    29041 cgcctgcacg cgtctccacc acgctgggta actgatgatt cggttgtacc cggaacaact
    29101 ccgcgcgcag ctcaatgaag ggctgcgcgc ggcgtatctt ttacttggta acgatcctct
    29161 gttattgcag gaaagccagg acgctgttcg tcaggtagct gcggcacaag gattcgaaga
    29221 acaccacact ttttccattg atcccaacac tgactggaat gcgatctttt cgttatgcca
    29281 ggccatgagt ctgtttgcca gtcgacaaac gctattgctg ttgttaccag aaaacggacc
    29341 gaatgcggcg atcaatgaac aacttctcac actcaccgga cttctgcatg acgacctgct
    29401 attgatcgtc cgcggtaata aattaagcaa agcgcaagaa aattccgcct ggtttactgc
    29461 tcttgcgaat cgcagcgtgc aggtgacctg tcagacaccg gagcaggctc agcttccccg
    29521 ctgggttgct gcgcgcgcaa aacagctcaa tttagaactg gatgacgcgg cgaatcaggt
    29581 gctctgctac tgttatgaag gtaacctgct ggcgctggct caggcactgg agcgtttatc
    29641 gctgctctgg ccagacggca aattgacatt accgcgcgtt gaacaggcgg tgaatgatgc
    29701 cgcgcatttc accccttttc attgggttga tgctttgttg atgggaaaaa gtaagcgcgc
    29761 gttgcatatt cttcagcaac tgcgtctgga aggcagcgag ccggttattt tgttgcgcac
    29821 attacaacgt gaactgttgt tactggttaa cctgaaacgc cagtctgccc atacgccact
    29881 gcgtgcgttg tttgataagc atcgggtatg gcagaaccgc cggggcatga tgggcgaggc
    29941 gttaaatcgc ttaagccagc cgcagttacg tcaggctgtg caactcctga cacgaacgga
    30001 actcaccctc aaacaagatt acggtcagtc agtgtgggca gagctggaag ggttatctct
    30061 tctgttgtgc cataaacccc tggcggacgt atttatcgac ggttgatatg aaatctttac
    30121 aggctctgtt tggcggcacc tttgatccgg tgcactatgg tcatcttaaa cccgtggaaa
    30181 cgctggcgaa tttgattggt ctgacgcggg tcacaataat ccctaataat gttcctccgc
    30241 atcgtcccca gccggaagcg aacagcgtgc agcgtaaaca catgcttgaa ctggcgattg
    30301 ccgacaagcc attatttact cttgatgaac gcgagctaaa gcgcaatgcc ccctcttaca
    30361 ctgcgcaaac actgaaagag tggcggcagg aacaagggcc ggacgtgccg ctggcgttta
    30421 ttattggtca ggattcattg ctgacctttc cgacctggta cgaatacgaa acgatactcg
    30481 acaatgcaca tttgatcgtc tgtcggcgtc caggttaccc acttgaaatg gcgcaaccgc
    30541 aataccagca atggctggaa gatcatttga cacataaccc ggaagatctt caccttcagc
    30601 ctgccggtaa aatttatctg gctgaaacac cgtggtttaa catctcggcg accatcatcc
    30661 gcgaacgttt gcaaaacggt gaatcgtgtg aggatttatt gccggaaccg gtattgactt
    30721 acattaacca acaaggcttg tatcgctgat acctgtcgtt acggagggca tgatgcgact
    30781 gtggttaatt cgtcatggcg aaacacaagc gaatgtcgat ggtctgtaca gcggtcatgc
    30841 gcccacaccc ctgaccgcac gcggtattga gcaagcgcaa aatctgcata cactgctaca
    30901 tgatgtttcc tttgacctgg ttttatgcag tgaactggaa cgggcacagc ataccgcgcg
    30961 actggttctc agtgaccgcc agctccccgt tcatatcatc cctgaactca acgaaatgtt
    31021 ttttggtgac tgggagatgc gacatcatcg cgacctcatg caagaagatg ccgaaaacta
    31081 tagcgcgtgg tgcaatgact ggcagcatgc tatccccacg aacggtgaag gatttcaggc
    31141 attttcacaa cgtgtggaac gctttatcgc aaggcttagt gaatatcagc actatcagaa
    31201 tattttaatc gtcagccatc agggcgtact gagtctgtta attgctcgtt taattggcat
    31261 gcctgccgag tctatgtggc attttcgcgt tgaccagggg tgttggagcg ccattgatat
    31321 caaccaaaaa ttcgcgacgc tacgcgtcct caatagccgt gccatcgggg tcgagaatgc
    31381 atgacttttc tgttttttta cgggtaagcc gcaacgacca ttgacagccc cgggcaggct
    31441 gatattctcc gcagccagac tttttccgcc agacacgact ttgtagaaat tgttttacaa
    31501 aaatggcgat gcaatctgcg gcgcggggtg ggatgatagc ccactttcga aagccgattc
    31561 ggcgacaatt gtcccgaaat cgcctctggt tcaggtatac tgacagacca tttttatcta
    31621 tttgattcac ccagggggaa aacttgcagg gtaaagcact ccaggatttt gttatcgaca
    31681 aaattgatga cctcaaaggt caggacatca tcgccttaga cgttcaaggc aaatccagca
    31741 tcaccgactg catgatcatc tgtacgggta cgtccagccg tcatgttatg tccattgctg
    31801 accacgttgt gcaggagtct cgcgcagcgg gcctgttacc gctcggcgta gaaggtgaaa
    31861 acagcgccga ctggattgtc gtggatttgg gcgatgtgat tgtccatgtc atgcaggaag
    31921 agagccgtcg cctgtatgaa ctggaaaaac tctggagtta atgcgtgaag ctgcaacttg
    31981 tcgccgtggg aacgaaaatg ccggactggg tacaaaccgg ttttaccgag tacctgcgtc
    32041 gttttccgaa agatatgccc ttcgagctga ttgaaattcc ggccggaaaa cgcggcaaga
    32101 atgcggacat caagcgcata ctcgacaaag agggtgagca gatgttggcg gccgcaggca
    32161 aaaaccgcat tgtcaccctc gatattccag gcaagccctg ggatacgccg cagttagccg
    32221 ctgagctgga acgctggaag ctggatggtc gcgacgtcag tctactgatt ggcgggcctg
    32281 aagggttgtc gcctgcctgt aaagcggcgg cggagcagag ctggtcgctg tcggcgctta
    32341 ccctccccca tccgctggtt cgcgtgctgg tcgcagagag tctgtaccgg gcgtggagca
    32401 tcaccaccaa ccatccttat caccgtgagt gataagggag ctttgagtag aaaacgcagc
    32461 ggatgaaact acagaactct tttcgcgact atacggcaga gtccgcgctg tttgtgcgcc
    32521 gggcgctggt cgcctttttg gggattttgc tgctgaccgg cgtgcttatc gccaacctgt
    32581 ataatctgca aattgttcgc tttaccgact accagactcg ctctaatgaa aaccgcatta
    32641 agctggtgcc tatcgcgccc agccgcggca ttatctacga ccgtaatggt atccctctgg
    32701 ccctcaaccg cactatctac cagatagaaa tgatgccaga gaaagtcgat aacgtgcagc
    32761 aaacgctgga cgctttgcgc agcgtggttg atctgaccga tgacgatatt gctgcattcc
    32821 gaaaagagcg cgcacgttca caccgtttca cctctattcc ggtgaaaact aacctgaccg
    32881 aagtacaagt agctcgcttt gccgtcaatc agtaccgttt tccgggtgtc gaagttaaag
    32941 gctataaacg tcgttactat ccttacggtt cggcgttgac ccacgtcatc ggctatgtgt
    33001 cgaaaatcaa cgataaagac gtcgaacgcc tgaataatga cggcaaactg gccaactatg
    33061 cggcaacgca tgatatcggt aagctgggca ttgagcgtta ctatgaagac gtgctgcacg
    33121 gtcagaccgg ttatgaagag gttgaagtta acaaccgtgg gcgtgttatc cgccagttaa
    33181 aagaagtacc accgcaagcc ggacacgata tttacctgac gctggatctc aaactccagc
    33241 aatatattga aacgctgctg gcgggtagcc gcgcagctgt ggttgtcacc gatccgcgta
    33301 caggtggggt actggcgctg gtttccacgc ctagttatga cccaaacctg tttgttgacg
    33361 gtatctccag caaagattat tccgccttgt tgaacgaccc gaatacaccg ctggtgaacc
    33421 gcgccacaca gggggtttat cctcccgcgt ctacagttaa accctatgtg gcggtttcgg
    33481 cattgagcgc cggggtgatc acgcgcaata cgacgctgtt tgacccaggc tggtggcaac
    33541 tgccaggttc ggaaaaacgt tatcgtgact ggaaaaaatg gggccacggg cgtctgaatg
    33601 tcacaagatc gctggaagaa tctgcggata ccttcttcta tcaggtggcc tacgatatgg
    33661 ggatcgatcg cctctccgaa tggatgggta aattcggtta tggtcattac accggtatcg
    33721 acctggcgga agaacgttcc ggcaacatgc ctacccgcga atggaaacag aaacgcttta
    33781 aaaaaccgtg gtatcagggt gacaccattc cggttggtat cggtcagggt tactggacag
    33841 cgaccccaat ccagatgagt aaggcactga tgatcctgat taatgacggt atcgtgaagg
    33901 ttcctcattt gctgatgagc accgccgaag acggcaaaca ggtgccatgg gtacagccgc
    33961 atgaaccgcc cgtcggcgat attcattccg gttactggga gctggcgaaa gacggtatgt
    34021 acggtgttgc taaccgccct aacggtacgg cgcataaata ctttgccagc gcaccgtaca
    34081 aaattgcggc gaaatccggt accgctcagg tcttcggtct gaaagcgaac gaaacctata
    34141 atgcgcacaa aattgccgag cgtttacgtg accacaaact gatgaccgcc tttgcgccat
    34201 acaacaatcc gcaagtggct gtcgccatga ttctggagaa cggtggtgcg ggtccggcgg
    34261 ttggtacact gatgcgccag atcctcgacc acattatgct gggtgataac aacaccgatc
    34321 tgcctgcgga aaatccagcg gttgccgcag cggaggacca ttaatcatga cggataatcc
    34381 gaataaaaaa acattctggg ataaagtcca tctcgatccc acaatgctgc tgatcttact
    34441 ggcattgctg gtttacagcg ccctggttat ctggagcgcc agcggtcagg atattggcat
    34501 gatggagcgt aaaatcggcc aaatcgcgat gggtctggtc atcatggtgg tgatggcgca
    34561 aattcctcca cgcgtttatg aaggctgggc cccctatctc tatatcatct gtattatttt
    34621 gctggtggcg gtagatgctt tcggtgccat ctctaaaggt gctcaacgct ggctggacct
    34681 cggtattgtt cgttttcagc cgtcggaaat tgccaaaata gccgtaccac tgatggttgc
    34741 gcgctttatc aaccgcgacg tttgcccgcc atcgttgaag aacactggca tcgcgctggt
    34801 gctgatattt atgcccacgc tgctggtggc tgcacagcct gacctgggaa catcaatcct
    34861 cgttgcgctt tccggtctgt ttgtactgtt cctctctggc cttagctggc gtctgattgg
    34921 cgtcgcagta gtgctggtgg cggcgttcat tccgattctg tggttcttcc tgatgcatga
    34981 ttaccagcgc cagcgcgtaa tgatgctcct ggacccggaa tcagacccac tcggcgcggg
    35041 ctatcacatt attcagtcta aaattgctat tggctccggc ggattacgcg gcaaaggctg
    35101 gctgcacggc actcagtcac agcttgaatt tctccccgaa cgccataccg actttatctt
    35161 cgcggtactg gcggaagagc tgggattagt gggcattctg attctgctcg ctctctacat
    35221 tctgctgatc atgcgcgggc tgtggatagc cgccagagcg caaaccacct ttggtcgcgt
    35281 catggctggt ggcttaatgc tgatattatt cgtttatgtc ttcgtaaata ttggtatggt
    35341 aagcggtatt ctgccggttg taggggttcc gctcccactg gtcagttatg gaggatcggc
    35401 gctaattgtg ctgatggctg ggttcgggat tgtaatgtca atccacaccc acaggaaaat
    35461 gttgtcgaaa agcgtgtaag aggtgcgcaa tgcgtaagca gtggctcggg atctgcatcg
    35521 cggcaggaat gctcgcggca tgtacaagcg atgatggtca gcaacagacg gtaagtgtac
    35581 cgcagcctgc ggtatgtaac ggccccatag ttgaaattag cggggcggac ccgcgtttcg
    35641 aaccactgaa cgcgacggca aatcaggatt accagcgcga cggtaaaagc tacaaaatcg
    35701 tgcaggatcc gtctcgattt agccaggcgg gactggcggc aatctatgat gccgaaccag
    35761 gcagtaacct gacggcctct ggcgaagctt tcgatccgac acagctgacg gcggcccatc
    35821 caacgcttcc gatccccagc tacgccagaa tcactaacct ggctaacggg cgaatgatcg
    35881 tggtgcgcat taatgatcgc ggcccttacg gcaacgaccg cgttatttcg ctttctcgcg
    35941 cggcagctga ccgtcttaac acgtcaaaca acaccaaagt tcgtatcgat ccgattattg
    36001 tcgcccagga tggttcgctt tctggtcctg gtatggcttg taccacagtc gccaaacaga
    36061 cttacgccct gcctgcacct cccgatttaa gcggtggcgc gggaacaagt tcagtgtctg
    36121 gcccgcaggg tgacattctt ccggtcagta attcgacgct aaaaagcgaa gatccgaccg
    36181 gcgcgccggt aaccagtagc ggtttcctcg gcgcaccaac gaccttagcg cctggtgtac
    36241 tggaaggcag cgaaccgacg cctgcgccac agcccgttgt tacagctccg tcgacaacgc
    36301 ctgcaacctc gcctgcaatg gtgacaccgc aagccgcctc gcaaagcgcc agcggcaact
    36361 ttatggtgca agtcggggcc gttagcgatc aggctcgtgc gcaacagtac caacagcaac
    36421 tgggacagaa gttcggcgtc cccggtcgcg taactcaaaa tggcgcggtc tggcggatcc
    36481 agcttggccc cttcgccagc aaagccgaag ccagtacctt gcagcaacgt ttgcaaaccg
    36541 aagcccaatt acagtcattt attaccaccg cgcagtagcg taaagcaggc atctgatgtg
    36601 tcaatttctg taaatgacgt taacaaagtc acgcggaaag tcagatgcct gccggtagtg
    36661 catttgctat agtagggcac tttttttaat tccatcacgg atgtcgtagt tcagaccatg
    36721 aataccattt tttccgctcg tatcatgaag cgcctggcgc tcaccacggc tctttgcaca
    36781 gcctttatct ctgctgcaca tgccgatgac ctgaatatca aaactatgat cccgggtgta
    36841 ccgcagatcg atgcggagtc ctacatcctg attgactata actccggcaa agtgctcgcc
    36901 gaacagaacg cagatgtccg ccgcgatcct gccagcctga ccaaaatgat gaccagttac
    36961 gttatcggcc aagcaatgaa agccggtaaa tttaaagaaa ctgatttagt cactatcggc
    37021 aacgacgcat gggccaccgg taacccggtg tttaaaggtt cttcgctgat gttcctcaaa
    37081 ccgggcatgc aggttccggt ttctcagctg atccgcggta ttaacctgca atcgggtaac
    37141 gatgcttgtg tcgccatggc tgattttgcc gctggtagcc aggacgcttt tgttggcttg
    37201 atgaacagct acgttaacgc cctgggcctg aaaaacaccc acttccagac ggtacatggt
    37261 ctggatgctg atggtcagta cagctccgcg cgagatatgg cgctgatcgg ccaggcattg
    37321 atccgtgacg taccgaatga atactcgatc tataaagaaa aagaatttac gtttaacggt
    37381 attcgccagc tgaaccgtaa cggcctgtta tgggataaca gcctgaatgt cgacggcatc
    37441 aaaaccggac acactgacaa agcaggttac aaccttgttg cttctgcgac tgaaggccag
    37501 atgcgcttga tttctgcggt gatgggcgga cgtactttta aaggccgtga agccgaaagt
    37561 aaaaaactgc taacctgggg cttccgtttc ttcgaaaccg ttaacccact gaaagtaggt
    37621 aaagagttcg cctctgaacc ggtttggttt ggtgattctg atcgcgcttc gttaggggtt
    37681 gataaagacg tgtacctgac cattccgcgt ggtcgcatga aagatctgaa agccagctat
    37741 gtgctgaaca gcagtgaatt gcatgcgccg ctgcaaaaga atcaggtcgt cggaactatc
    37801 aacttccagc ttgatggcaa aacgatcgag caacgcccgc tggttgtgtt gcaagaaatc
    37861 ccggaaggta acttcttcgg caaaatcatt gattacatta aattaatgtt ccatcactgg
    37921 tttggttaaa aattgaacac ttgaaagtgt aatttccgtc cccatatact aagcatcagt
    37981 aaaaaactcc cgccttctgg cgggagttgc tatttaatta cgttacgccg gagctgacat
    38041 gaaaaccaaa cttaacgaac tgcttgaatt ccctactcct tttacttaca aagttatggg
    38101 gcaggcgtta cctgagctgg ttgatcaggt ggttgaagtg gtacagcgcc atgcgccagg
    38161 tgactacacc ccaacggtaa aaccaagcag caaaggcaac taccactcgg tatctatcac
    38221 tatcaacgcc actcatatcg agcaggttga aacactgtat gaagaactgg gcaaaatcga
    38281 tattgtccgc atggttctgt aactcgcttc tccgttaccc ggctcctggt cgggtaactc
    38341 ccctctcctc gctgtgatat acttttcccc cacttttact cattctccac ggagatgccg
    38401 ttttgtatca ggataaaatt cttgtccgcc agctcggtct tcagccttac gagccaatct
    38461 cccaggctat gcatgaattc accgataccc gcgatgatag tacccttgat gaaatctggc
    38521 tggtcgagca ctatccggta ttcacccaag gtcaggcagg aaaagcggag cacattttaa
    38581 tgccgggtga tattccggtg atccagagcg atcgcggtgg gcaggtgact tatcacgggc
    38641 cggggcaaca ggtgatgtat gtgttgctta acctgaaacg ccgtaaactc ggtgtgcgtg
    38701 aactggtgac cttgcttgag caaacagtgg tgaataccct ggctgaactg ggtatagaag
    38761 cgcatcctcg ggctgacgcg ccaggtgtct atgttgggga aaagaaaatt tgctcactgg
    38821 gtttacgtat tcgacgcggt tgttcattcc acggtctggc attaaacgtc aatatggatc
    38881 tttcaccatt tttacgtatt aatccttgtg ggtatgccgg aatggaaatg gctaaaatat
    38941 cacaatggaa acccgaagcg acgactaata atattgctcc acgtttactg gaaaatattt
    39001 tagcgctact aaacaatccg gacttcgaat atattaccgc ttaattccat acatcaatgg
    39061 cccaatttac actgggtcat tactcataat acctgctaat gctttacatc tccttcgcgt
    39121 ttgtatattt tctttatcac ttgcagaaat gcggtgccac gccttgctcc gtaactcacc
    39181 ccattgatta acctattgaa atagttgatt atggtgagca aaagcgttcc gttccaatta
    39241 gacataagat tattgtaatt ttttaaatat tatt
//
LOCUS       DAPHBJ010000019        38080 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.019, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000019 DAPHBJ010000000
VERSION     DAPHBJ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.019"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            202..1008
                     /gene="xthA"
                     /locus_tag="RHZ76_000917"
     CDS             202..1008
                     /gene="xthA"
                     /locus_tag="RHZ76_000917"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDV2811602.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDKEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            1175..1885
                     /locus_tag="RHZ76_000918"
     CDS             1175..1885
                     /locus_tag="RHZ76_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDV2811603.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            1890..2567
                     /gene="ydjY"
                     /locus_tag="RHZ76_000919"
     CDS             1890..2567
                     /gene="ydjY"
                     /locus_tag="RHZ76_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HDV2811604.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRIDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYIYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            2582..3289
                     /locus_tag="RHZ76_000920"
     CDS             2582..3289
                     /locus_tag="RHZ76_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDV2811605.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            3289..3837
                     /locus_tag="RHZ76_000921"
     CDS             3289..3837
                     /locus_tag="RHZ76_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDV2811606.1"
                     /translation="MGLPPLSKIPLILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPTQGLCRIPEKRS"
     gene            3847..5013
                     /locus_tag="RHZ76_000922"
     CDS             3847..5013
                     /locus_tag="RHZ76_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV2811607.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTVPLWQYLDVLHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRESLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            4986..6521
                     /locus_tag="RHZ76_000923"
     CDS             4986..6521
                     /locus_tag="RHZ76_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2811608.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKWL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDIDQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGVRKP
                     ATPLLPGNTLAIFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPTLIAFAVGFAVGIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            6521..7174
                     /locus_tag="RHZ76_000924"
     CDS             6521..7174
                     /locus_tag="RHZ76_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDV2811609.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAEQFSCTGELWLNEQRIDILPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLEGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            7241..8548
                     /gene="ynjE"
                     /locus_tag="RHZ76_000925"
     CDS             7241..8548
                     /gene="ynjE"
                     /locus_tag="RHZ76_000925"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HDV2811610.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(8557..9177)
                     /locus_tag="RHZ76_000926"
     CDS             complement(8557..9177)
                     /locus_tag="RHZ76_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDV2811611.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            9264..9671
                     /gene="nudG"
                     /locus_tag="RHZ76_000927"
     CDS             9264..9671
                     /gene="nudG"
                     /locus_tag="RHZ76_000927"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HDV2811612.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(9637..9909)
                     /locus_tag="RHZ76_000928"
     CDS             complement(9637..9909)
                     /locus_tag="RHZ76_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HDV2811613.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            10145..11488
                     /gene="gdhA"
                     /locus_tag="RHZ76_000929"
     CDS             10145..11488
                     /gene="gdhA"
                     /locus_tag="RHZ76_000929"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDV2811614.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSLPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMMAQGVI"
     gene            complement(11605..12645)
                     /locus_tag="RHZ76_000930"
     CDS             complement(11605..12645)
                     /locus_tag="RHZ76_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDV2811615.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANHSSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSQMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(12773..14734)
                     /gene="topB"
                     /locus_tag="RHZ76_000931"
     CDS             complement(12773..14734)
                     /gene="topB"
                     /locus_tag="RHZ76_000931"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDV2811616.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDCLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVTPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            complement(14739..15782)
                     /gene="selD"
                     /locus_tag="RHZ76_000932"
     CDS             complement(14739..15782)
                     /gene="selD"
                     /locus_tag="RHZ76_000932"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDV2811617.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(15899..16450)
                     /locus_tag="RHZ76_000933"
     CDS             complement(15899..16450)
                     /locus_tag="RHZ76_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HDV2811618.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            16611..18467
                     /gene="sppA"
                     /locus_tag="RHZ76_000934"
     CDS             16611..18467
                     /gene="sppA"
                     /locus_tag="RHZ76_000934"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDV2811619.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAEQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            18634..19650
                     /gene="ansA"
                     /locus_tag="RHZ76_000935"
     CDS             18634..19650
                     /gene="ansA"
                     /locus_tag="RHZ76_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDV2811620.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            19661..20302
                     /gene="pncA"
                     /locus_tag="RHZ76_000936"
     CDS             19661..20302
                     /gene="pncA"
                     /locus_tag="RHZ76_000936"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HDV2811621.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(20395..21753)
                     /locus_tag="RHZ76_000937"
     CDS             complement(20395..21753)
                     /locus_tag="RHZ76_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2811622.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWLLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLLLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(21871..22629)
                     /locus_tag="RHZ76_000938"
     CDS             complement(21871..22629)
                     /locus_tag="RHZ76_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV2811623.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVVLLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(22766..23746)
                     /gene="ydjG"
                     /locus_tag="RHZ76_000939"
     CDS             complement(22766..23746)
                     /gene="ydjG"
                     /locus_tag="RHZ76_000939"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HDV2811624.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            complement(23756..24703)
                     /locus_tag="RHZ76_000940"
     CDS             complement(23756..24703)
                     /locus_tag="RHZ76_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HDV2811625.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRVGKDAAGQFILDHCRKENIDIQSVKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNYAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            complement(24708..25544)
                     /locus_tag="RHZ76_000941"
     CDS             complement(24708..25544)
                     /locus_tag="RHZ76_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDV2811626.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            complement(25565..26608)
                     /locus_tag="RHZ76_000942"
     CDS             complement(25565..26608)
                     /locus_tag="RHZ76_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HDV2811627.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLIMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            complement(26625..28004)
                     /locus_tag="RHZ76_000943"
     CDS             complement(26625..28004)
                     /locus_tag="RHZ76_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2811628.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVWLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIVVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            complement(28031..29107)
                     /locus_tag="RHZ76_000944"
     CDS             complement(28031..29107)
                     /locus_tag="RHZ76_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDV2811629.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVKLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            complement(29477..29749)
                     /locus_tag="RHZ76_000945"
     CDS             complement(29477..29749)
                     /locus_tag="RHZ76_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HDV2811630.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(29791..30204)
                     /gene="msrB"
                     /locus_tag="RHZ76_000946"
     CDS             complement(29791..30204)
                     /gene="msrB"
                     /locus_tag="RHZ76_000946"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDV2811631.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            30546..31541
                     /gene="gapA"
                     /locus_tag="RHZ76_000947"
     CDS             30546..31541
                     /gene="gapA"
                     /locus_tag="RHZ76_000947"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDV2811632.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            31625..32509
                     /locus_tag="RHZ76_000948"
     CDS             31625..32509
                     /locus_tag="RHZ76_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDV2811633.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKENVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(32557..33411)
                     /gene="yeaE"
                     /locus_tag="RHZ76_000949"
     CDS             complement(32557..33411)
                     /gene="yeaE"
                     /locus_tag="RHZ76_000949"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HDV2811634.1"
                     /translation="MQQKMIQFSGDVSLPAVGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(33501..34247)
                     /gene="mipA"
                     /locus_tag="RHZ76_000950"
     CDS             complement(33501..34247)
                     /gene="mipA"
                     /locus_tag="RHZ76_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HDV2811635.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            34683..36617
                     /gene="yeaG"
                     /locus_tag="RHZ76_000951"
     CDS             34683..36617
                     /gene="yeaG"
                     /locus_tag="RHZ76_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HDV2811636.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            36730..38013
                     /locus_tag="RHZ76_000952"
     CDS             36730..38013
                     /locus_tag="RHZ76_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HDV2811637.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT         9529 a         9828 c         9206 g         9517 t
ORIGIN      
        1 attctggaat tgtgatcatt gacgaaattt actggaaatt actgcgccat tctgacgcag
       61 cgcgcaccaa aagcgggcat tttttgcgcc atcgttgaca tcattaacaa ccatcgatca
      121 aatcacttaa caacaggcgg taagcaacgc gaaattctgc taccatccac gcactcttta
      181 tctgaataaa tggcagcgac tatgaaattt gtctctttta atatcaacgg cctgcgcgcc
      241 agacctcacc agcttgaagc catcgtcgaa aagcaccaac cggatgtgat tggcctgcag
      301 gagacaaaag ttcatgacga tatgtttccg ctcgaagagg tggcgaagct cggctacaac
      361 gtgttttatc acgggcagaa aggccattat ggcgtggcgc tgctgaccaa agagacgccg
      421 attgccgtac gtcgcggctt tcccggtgac gacaaagagg cgcagcggcg gattattatg
      481 gcggaaatcc cctcactgct gggtaatgtc accgtgatca acggttactt cccgcagggt
      541 gaaagccgcg accatccgat aaaattcccg gcaaaagcgc agttttatca gaatctgcaa
      601 aactacctgg aaaccgaact caaacgtgat aatccggtac tgattatggg cgatatgaat
      661 atcagcccta cagatctgga tatcggcatt ggcgaagaaa accgtaagcg ctggctgcgt
      721 accggtaaat gctctttcct gccggaagag cgcgaatgga tggacaggct gatgagctgg
      781 gggttggtcg ataccttccg ccatgctaat ccgcaaacag cagatcgttt ctcatggttt
      841 gattaccgct caaaaggttt tgacgataac cgtggtctgc gcatcgacct gctgctcgcc
      901 agccaaccgc tggcagaatg ttgcgtagaa accggcatcg actatgaaat tcgcagcatg
      961 gaaaaaccgt ccgatcacgc ccccgtctgg gcgaccttcc gccgctaatt tagcagctct
     1021 cctggctcaa actgggtcag gagaattaac cttgagaaaa atcaacaaac tgtcagtaat
     1081 gatttgttgc ctgccgtcct ttgttatacc gtcactgcgt ttttagttgt ctgaccactt
     1141 ctctattatc aagtttgata taggaaactc cacgatgaac gctgagcgta actttctgtt
     1201 tgcctgtctt atttttgcgc tggtcattta cgctatccac gctttcggtt tattcgatct
     1261 gctcaccgat ttaccccact tacagacact cattcgccag agcggattgt tcggttatag
     1321 cctctatatt ctgttattca tcattgccac cctctttctg ttaccaggaa gcatactggt
     1381 gatcgctgga ggaatagttt ttggcccgct cttagggaca ctactctcat taattgccgc
     1441 aacgctggcc tcctcgtgct cattcctgct ggcgcgctgg ctgggacgtg atttactgct
     1501 gaaatacgtg ggccatagcc ataccttcca ggccattgaa aaaggcattg cgcgtaacgg
     1561 tattgatttt cttattctga cccgcttaat cccgttgttt ccttacaata ttcaaaatta
     1621 cgcttacgga ttaaccacca tcgccttctg gccttatacc cttatttcgg cactcacgac
     1681 cctgcccggt attgttattt ataccgtgat ggcaagcgat ctcgccaatg aaggcattac
     1741 gctgcgcttt attttacaac tctgtctggc gggcctggcg ctgtttattc tcgtccagct
     1801 cgcaaaactc tacgcccgac acaaacatgt ggatctgtct gcttcgcgcc gcagcccact
     1861 tactcaccca aaaaatgaag gatagaacga tgtcgcaaca ttattcagtg tcatggaaaa
     1921 aaggactggc tgcactctgc ttactggctg ttgccgggct tagcggctgc gatcaaaaag
     1981 agaatgcggc ggcaaaagtg gaatacgacg gactttcgaa cagccaaccg ctgcgcatcg
     2041 atgccaataa ccatacggta accatgctgg tgcaaattaa tggtcgtttc ctcaccgacg
     2101 acactcgtca cggtattgtg tttaaagatg gctccaacgg acataaatcg ctgtttatgg
     2161 cttatgcgac cccgaaagca ttttatgaag ccctgaaaga ggcaggtggt acgccgggcg
     2221 aaaacatgac gatggataat aaagaaacga ctcatgtcac aggcagcaaa ctggatattt
     2281 cggtcaactg gcaaggggcg gcaaaagcgt attccttcga tgaagtgatt gttgacagta
     2341 atggcaagaa actggacatg cgctttggcg gtaatttaac ggcagcagaa gagaagaaaa
     2401 cgggttgcct ggtgtgtctg gatagctgcc cggtcggcat cgtcagcaat gcaacataca
     2461 tttatggtgc ggtggaaaaa cgcggcgaag tgaaattcaa aggcaatgcc tcagttctcc
     2521 cggcggataa cacgctggca acggttacct ttaaaatcac cgaataaagc caggataaag
     2581 gatgatgaag atacaatcgc gtaaaatctg gtactaccgt ataaccctca tcatcctgtt
     2641 gttcgctatg ctgctcgcat gggcgctgct tcccggcgtc catgagttta tcaatcgcag
     2701 cgttgcggcg tttgccgccg tggaccaaca gggtatagaa cgctttattc agtcttacgg
     2761 cgcactggcg gcagttgtct cgttcttgtt gatgatttta caggccattg ctgcaccgct
     2821 acctgcgttt ttgatcacct ttgccaatgc gtcgctgttt ggcgcgttct ggggcggctt
     2881 gctgtcgtgg accagttcga tggccggcgc ggcgctgtgc ttttttatcg ccagagtgat
     2941 gggccgcgaa gtggtggaaa aattaaccgg caaaaccgtg cttgacagta tggacggctt
     3001 tttcactcgc tacggcaaac acaccattct ggtctgtcgg ttattgcctt ttgtcccttt
     3061 cgatccaatc agctatgctg ccggtttgac ttcaatacgt tttcgctcgt tttttatcgc
     3121 caccgggctt ggtcagttac cggcaactat tgtttattcc tgggcgggca gcatgttaac
     3181 aggcggtact ttctggtttg tcaccggact gtttattctg tttgccctga ccgtggtgat
     3241 ttttatggcg aagaaaatat ggcttgaacg ccagaagagg aatgcctgat gggtttaccg
     3301 ccgcttagca aaattccttt aattttacgt ccacaggcgt ggctgcatcg tcgccattac
     3361 ggcgaggtgc taagccccat tcgctggtgg gggcggatcc cgtttatctt ttatctggtg
     3421 tcgatgtttg ttggctggct ggagcgcaaa cgctcaccgc tcgatccggt agtacgatcg
     3481 cttgtcagcg cgcgcattgc gcaaatgtgc ctgtgtgagt tttgtgtgga tatcaccagt
     3541 atgaaagtcg ccgagcgcac cggcagcagc gataaactgc tggcagtggc tgactggcgg
     3601 caaagcccgc tctttagcga tgaagaacgg ctggcgctgg agtacgccga agccgcaagc
     3661 gtaacgccgc caacggtcga tgatgccctg cgtacccgac tggctgcgca ttttgacgct
     3721 caggcgctca ccgaactgac ggcattgatc ggcctgcaaa atctgtcagc ccgttttaat
     3781 tctgccatgg acattcccac tcaggggctg tgccgtattc ctgaaaaacg ttcttaagga
     3841 gagatgatgc gccattgtgg gtggttgctg ggattgttat cgctgttttc tctggcaaca
     3901 catgccagtg actggcaaga aattaaaaat gaggccaaag ggcaaaccgt ctggtttaac
     3961 gcctggggcg gcgataccgc aattaaccgc tatctcgact gggtgagcgg cgagatgaaa
     4021 acccattacg ctataaacct gaagattgtc cgtctggcgg atgccgcaga cgcggtgaag
     4081 cgcattcaga ccgaagccgc agccggacgt aaaacgggcg gctcggtgga tctgctctgg
     4141 gtgaacggcg aaaacttccg caccttaaaa gaggccaatt tattacaaac gggctgggcg
     4201 gagactctgc ccaactggcg ctatgtcgac acacagctgc cggtgcggga agatttttct
     4261 gtgccgacac aaggtgcgga atcgccctgg ggcggcgcac aactgacgtt tatcgcccgc
     4321 cgcgatgtta cgccacagcc accacaaacg ccgcaagcct tactggagtt tgctaaagcc
     4381 aatcccggca cggttaccta tccgcgccca ccggacttta ccggcacggc gtttcttgaa
     4441 cagttgctga ttatgctgac gcccgatccc gccgcattaa aagaagcgcc ggacgatgcg
     4501 actttcgccc gtgtcactgt gccattgtgg caatatcttg atgtgctgca tccgtatttg
     4561 tggcgcgaag gaaaggattt cccgccttcg cccgcgcgga tggatgctct gctgaaagcc
     4621 ggcacattgc gcctgtcgct gacctttaac cccgcgcatg cgcagcaaaa aatcgccagc
     4681 ggcgatttgc ctgcaagcag ttacagtttt ggctttcgcg aggggatgat tggcaacgtg
     4741 catttcgtca ccattcctgc caacgcgaat gccagtgctg cggcgaaggt agttgccaat
     4801 ttcctgctct cacccgatgc gcaactgcgt aaagcagatc ccgctgtctg gggcgatcct
     4861 tctgttctcg atccgcaaaa actgcctgac gggcagcgcg aatcattgca atcaagaatg
     4921 ccgcaggatc tgccgccggt actggctgaa ccgcacgcag gttgggtaaa tgcgctggaa
     4981 caagaatggc tacaccgtta cggtacgcat taatcttttt gctgtgggcg atggtggcgg
     5041 tgatttatgc accgctgatc ccggcagctc tcacgctgat atcgcctgct ttgtcgttga
     5101 cacactggca ggcgttattt gccgatccac agttaccgca ggcattactg gcaacgctgg
     5161 tgtcgacaac catcgcggcg gtcggggcat tgttgattgc cctgctggtg attgtggcgc
     5221 tgtggcctgg gccgaaatgg cagcgtatgt gcgcccgtct gccgtggctg ctcgccattc
     5281 cccatgtggc ttttgccacc agcgcccttc tgctctttgc tgacggaggg ctgctttatg
     5341 actatttccc gtatttcact ccgccaatgg accgatttgg catcggtctg ggcctcaccc
     5401 ttgcagtgaa agaaagcgca tttctgctgt ggatcttagc agcagtattg agcgaaaaat
     5461 ggctgttgca gcaggtcatt gtgctggatt cgctgggcta cagccgctgg caatgcctga
     5521 actggctgct gttgccctcc gtcgcgcctg cgctggcaat ggcgatgctg gcgattgttg
     5581 cctggtcgct gtcggtcgtg gatgtggcaa ttattctcgg gccaggtaat cccccgacgc
     5641 tggcggtaat tagctggcag tggttaaccc agggcgacat tgaccaacaa acaaaaggcg
     5701 cgcttgccag cctgctgttg atgctgttac tcgccgccta cgttttgctg agctatctgc
     5761 tatggcgcag ctggcggcgc actattcccc gcgtagatgg cgttcgcaag cctgccacgc
     5821 ctttattgcc gggcaatacg ctggcgattt ttttaccctt aaccggtgtg ctgtgtgtgg
     5881 ttctgctggc aatcctcgcg gatcagtcga cgatcaatag tgaagcgctc atcaacagcc
     5941 tgacaatggg gctggtggcg acattcatcg ctttgctcct gttactgctg tggctggaat
     6001 gggggccaca gcgtcgccag ttgtggctat ggttgcccat tttattaccc gctctgccac
     6061 tggtggcggg ccagtacacg ctggcgctat ggctgaacct ggatggaagc tggacggcgg
     6121 tggtctgggg gcatctgctg tgggtaatgc catggatgct gtttatcctg caacccgcct
     6181 ggcagcgcat tgattcacgg ttaattttga ttgcgcaaac actgggctgg tcgcgggcca
     6241 aaatcttctt ttacgtgaaa tgtccgctta tgctacgccc tacgctgatt gccttcgcgg
     6301 tgggatttgc agtcggtatt gcgcagtata tgccaacgct gtggctgggc gcggggcgtt
     6361 ttccgacgct caccactgag gcggtggcat taagcagcgg cggcagcaac ggtattctcg
     6421 ccgcccaggc tttatggcaa ctgctattac cgcttattat ttttgccctg accgcgttag
     6481 tcgcaaaatg ggtaggttat gtcagacaag gactccgcta atgctctgcg tgaaaaatgt
     6541 ttcgctacgt ttaccagaaa gccgcttgct gacaaacgtt aactttacgg tggataaagg
     6601 tgacattgtc acgttaatgg ggccgtctgg ctgtggaaaa tccactctgt tttcatggat
     6661 gattggtgca ctggccgaac agttttcttg tacaggtgag ctatggctca atgagcaacg
     6721 gattgacatc ctacccaccg cacagcgtca gattggcatt ctttttcagg atgcactgtt
     6781 atttgaccag ttcagtgtcg ggcaaaattt actgctggcg ctaccggcga cacttaaagg
     6841 gaatgcccga cgtaatgccg tgaatgatgc acttgagcgt tcaggccttg agggagcttt
     6901 ccatcaggat cctgccactt tgtctggcgg tcagcgagcg cgcgttgctc tactacgcgc
     6961 ccttctcgcc caaccaaaag cgttactcct ggatgagcca ttcagccgtc ttgatgtggc
     7021 tctgcgcgat aattttcgcc agtgggtgtt cagcgaagtt cgcgccctgg cgatccccgt
     7081 cgttcaggta acgcacgatc tccaggatgt tcctgctgat agttctgttc tggatatggc
     7141 gcagtggtca gaaaattaca acaaactgcg ataacgcaaa gtttttctca atgcgtcagt
     7201 tcagaatggc gcactcaaaa ctacaatgtc gggattttcg atgaaacgtg tttctcaaat
     7261 gaccgcgctg gcaatggctt tagggctggc ttgcgcttct tcgtgggccg ctgaactggc
     7321 gaagcctctt acacttgacc aacttcagca acaaaatggc aaagcgatag atactcgccc
     7381 cagcgcgttt tataacggct ggccacaaac cttaaatggc ccttctggtc atgaacctgc
     7441 cgccttaaac ctctctgcta gctggctcga caaaatgagc accgaacagc tcaacgcgtg
     7501 gatcaagcaa cataacctga aagccgatgc cccggtggcg ctgtacggta atgacaaaga
     7561 tgtcgacgcc gtcaaaacgc gactgcaaaa agcaggtttt acgcatatct ccattctgag
     7621 tgacgcgcta agcgaacctt cccgtctaca aaaactgccg cactttgagc agctggttta
     7681 tccgcaatgg ctgcacgacc tgcaacaagg taaagaggtt acggcgaaac ctgccggtga
     7741 ctggaaagtc attgaagcgg cctggggcgc tcctaagctt taccttatca gccatattcc
     7801 cggtgctgac tacatcgata ccaacgaagt ggaaagcgaa ccgctgtgga acaaagtttc
     7861 tgatgaacaa ctaaaagcga tgctggcaaa acacggcatt cgccatgaca ccacggtcat
     7921 tctgtacggg cgtgacgtat acgctggagc gcgtgtggcg caaattatgc tctacgcagg
     7981 cgtgaaagat gtgcgtctgc tggatggcgg ctggcaaacc tggtccgacg cgggactgcc
     8041 tgttgagcga ggaacgccac cgaaagtgaa agcggaaccg gatttcggcg tgaagatccc
     8101 ggcacaaccg cagttgatgc tcgatatgga acaggcccgt ggactgctgc atcgccagga
     8161 tgcatcgctg gtgagcattc gttcgtggcc agaatttatc ggtacgacca gcggttacag
     8221 ctatattaaa ccaaaaggtg aaatagccgg agcacgttgg ggacacgctg gtagcgactc
     8281 gacgcatatg gaagattttc ataacccgga tggcaccatg cgcagcgccg atgatattac
     8341 cgctatgtgg aaagcgtgga atatcaaacc agagcagcaa gtttcattct actgcggcac
     8401 cggctggcgc gcgtccgaaa cctttatgta cgcacgcgcc atgggttgga agaatgtttc
     8461 cgtgtatgac ggcggctggt acgaatggag cagcgatcca aaaaatccgg tagcaaccgg
     8521 tgaacgcggc ccggacagta gcaaataaca ttgcaattac tgacgctgga gcgacttcag
     8581 cgtcagatac ccactccaca cccgcgtaaa ggtcgtcatc cagcacaacg caccgaagat
     8641 ccacgcaaac cacgcaaacc atgcagggaa taaacatccc agcacaaaca gtaagatcgt
     8701 ttctgtgcct tcggttaatc cccccagata gtaaaacgac ttatgtgcat aaccggggtt
     8761 atcaatctga tgttttgctg ccagtgcagc aaaggcgaga aaactgctgc ccgtgccgat
     8821 aaacgcaaac aacaaccagc cgcctgccag cgcattttgc tccggtgcag caagaataaa
     8881 gccaaacggc accagcgcgt aaaagagaaa atcgagagaa atatcaagaa agccgcccgc
     8941 gtcggtaagc tctctgcgcc gcgccagcgc accgtccaga ccatcaagca gcctgttcaa
     9001 caaaatgacg actagcgccg ccagatacca gcccagcgcc agaaacggca gagccagcac
     9061 gccgatggca aatcccacta atgttaaacc atctggcgta atgcccggtt tatcaagaac
     9121 ccgcacgcat tgatgcaaca acggtttaat ccggggatga agatggcggt ctagcacagg
     9181 cactccttaa atataaagcc tttctgattg agcaacagtg cggatattat ggcatttttc
     9241 gcttatctgc ccgtgtgtaa tttatgaaaa tgattgaagt tgttgccgcc atcattgaac
     9301 gtgatggcaa aattttactc gcgcaacgcc ccgcccagag cgatcaggcg ggattatggg
     9361 agtttgccgg tggtaaagtc gagccggatg aaagtcagcg gcaggcgctg gtgcgtgagt
     9421 tacgcgaaga actgggcatc gaagcaactg tgggtgaata tgttgccagc catcagcgag
     9481 aagtttcggg gcggattatc catcttcatg cctggcacgt acccgacttc cacgggacgt
     9541 tacaggcaca tgaacatcag gcgctggtct ggtgctcacc tgaagaggcg ctgcaatatc
     9601 cgctggcccc tgctgacatt ccattattag aggcgtttat ggctttacgc gccgccagac
     9661 cagcggatta gtgctaaggg ttttgtcatc acgctggcat tgcagcagta ttccttcggc
     9721 tttaattacc gccccttcag aataattttg atcctgataa acgcagcact gagtacaggg
     9781 ctgcgctgac tgcccgcctg aactgaacac ttctggcggt acgtttacct ccacgtccgg
     9841 acgataatgc gggttagcca gtgcaattaa tggaaatgct aatactacgg cgaacaatgc
     9901 tcgactcaca gggaactcct taacgttatt gtctctgcta ctgataacgg tagccgggtg
     9961 gcaaaacttt agcgtctgag ttatcgcatt tggttatgag attactctcg ttattaattt
    10021 gctttcctgg gtcatttttt tcttgcttac cgtcacattc ttgatggtat agtcgaaaac
    10081 tgcaaaagca catgacataa acaacataag cacaatcgta ttaatatata agggttttat
    10141 atctatggat cagacatatt ctctggagtc attcctcaac catgtccaaa agcgcgaccc
    10201 gaatcaaacc gagttcgcgc aagccgttcg tgaagtaatg accacactct ggccttttct
    10261 tgaacaaaat ccaaaatatc gccagatgtc attactggag cgtctggttg aaccggagcg
    10321 cgtgatccag tttcgcgtgg tatgggttga tgatcgcaac cagatacagg tcaaccgtgc
    10381 atggcgtgtg cagttcagct ctgccatcgg cccgtacaaa ggcggtatgc gcttccatcc
    10441 gtcagttaac ctttccattc tcaaattcct cggctttgaa caaaccttca aaaatgccct
    10501 gactaccctg ccgatgggcg gtggtaaagg cggcagcgat ttcgatccga aaggaaaaag
    10561 cgaaggtgaa gtgatgcgtt tttgccaggc gctgatgacc gagctttatc gtcatctggg
    10621 cgcggatacc gacgttccgg caggtgatat cggggttggt ggtcgtgaag tcggctttat
    10681 ggcggggatg atgaaaaagc tctccaacaa taccgcctgc gtcttcaccg gtaagggcct
    10741 ttcatttggc ggcagtctta ttcgcccgga agctaccggc tacggtctgg tttatttcac
    10801 agaagcaatg ttaaaacgcc acggtatggg ttttgaaggg atgcgcgttt ccgtttctgg
    10861 ctccggcaac gtcgcccagt acgctatcga aaaagcgatg gaatttggtg ctcgtgtgat
    10921 cactgcgtca gactccagcg gcactgtagt tgatgaaagc ggattcacga aagagaaact
    10981 ggcacgtctt atcgaaatca aagccagccg cgatggtcga gtggcagatt acgccaaaga
    11041 atttggtctg gtctatctcg aaggccaaca gccgtggtct ctaccggttg atatcgccct
    11101 gccttgcgcc acccagaatg aactggatgt tgacgccgcg catcagctta tcgctaatgg
    11161 cgttaaagcc gtcgccgaag gggcaaatat gccgaccacc atcgaagcga ctgaactgtt
    11221 ccagcaggca ggcgtactat ttgcaccggg taaagcggct aatgctggtg gcgtcgctac
    11281 atcgggcctg gaaatggcac aaaacgctgc gcgcctgggc tggaaagccg agaaagttga
    11341 cgcacgtttg catcacatca tgctggatat ccaccatgcc tgtgttgagc atggtggtga
    11401 aggtgagcaa accaactacg tgcagggcgc gaacattgcc ggttttgtga aggttgccga
    11461 tgcgatgatg gcgcagggtg tgatttaagt tgtaaatgcc tgatggcgct acgcttatca
    11521 ggcctacaaa tgggcacaat tcattgcagt tacgctctaa tgtaggccgg gtaagcgcag
    11581 cgcccccggc aaaatttcag gcgtttatga gtatttaacg gatgatgctc cccacggaac
    11641 atttcttatg ggccaacggc atttcttact gtagtgctcc caaaactgct tgtcgtaacg
    11701 ataacacgct tcaagttcag catccgttaa ctttctgcga tagcagcaga tatgccagta
    11761 aagaaatccc atttgactat ttttttgata atcttcttcg ctttcgaaca actcgtgcgc
    11821 ctttcgagaa gcaagcatta tataatgcca ggccagttct tcttcaattg tcccgttttg
    11881 aaaagctgtg cttgatatcg agatcatcca tgataattcc gccgcccata ttagcttcgc
    11941 cgaggattta ccggagctat gattagcgca atcagagata tagtctgagg gaaaaacagc
    12001 aaatttattc aacaaggcga taacctgctc tggggcttcc tccatgtttg ctttaaaggt
    12061 attggctcca tggtcgccag aaagaaaatg ctccattaag gcacaataac tttcgctatc
    12121 ttcgataccc cattgatcct ctaaagactc gcgtctttta cttatgatat cgatcgagtc
    12181 aaaaggaagc acatgatatt ggaaggtatc tttgccaggt tcaggctttc gcggccagaa
    12241 ctccagcgtt tcagaccatt gcttatgata gaatcgataa ggtgcgatca attgtagcgc
    12301 ctgtaacttc tcgatactga gcggctcaat acctttagcc tgataataat gcagttgttc
    12361 tttttttgct ttaaaaccgg cccgaacaat aagccccatc ataattaata gataaagaaa
    12421 agagcatccc gcggtaatca ggcctctttc attcaaaccg ttggatgtta tcgctgcgaa
    12481 cacaaacatt acagcgacaa cacatgttaa ataaaacccc cacttacaaa gcagcatggc
    12541 cttattttct ttaatcatcc gttcaaaatt actattaaat atttcccagc cattaaaaga
    12601 atacttctcg ctcccaggat ggttttgtaa taaaactttt ttcatcacat tccctgttat
    12661 tacatacttt agaggcgata attttatcca tgcaaaaaaa atatccaacg aattttcttg
    12721 atccgctgga tatttcagaa tatgactcga atagcacgaa agattcactc gcttacgcta
    12781 tcgccccgct tccgacttca tctgctggcg gacttttttt cgcactacgt ttacgcggtg
    12841 cagccttttt cttatcagca ctgccaccac tgcccggagt cacaatgccg cgaaactgcc
    12901 gcaccggcgt acgtttggct tgatcaataa gctgatatag cgtccccacc agcggctgca
    12961 taaagtcctg atagcgacac tgcttttcgc tgatttgcgt cagcaccgat tcccagtgcg
    13021 cggtcatgtc cggtcgcgtc gccatctccg gcagcgaatg gaatagcgct tttccggcgt
    13081 cggtggagtg gatatagcgc ccttttttgg tcaggaaacc acgcttgaac aacagttcaa
    13141 taatcccggc acgcgttgcc tctgtcccca gaccatcggt cgcacggagg atctttttca
    13201 gatctttatc ctgcacaaag cgcgcgatcc cggtcatcgc cgaaagcagt gttgcatcgg
    13261 taaaatggcg cggcggctgg gtttgccgct ctaccacttc acctttttca cacagcaact
    13321 catcgccttt cgccaccaca ggcagtggcg tgccgtcgtt ttcttcatcg cgctctttgc
    13381 tgcctaacag cgtgcgccag cctgcttcag caagaaaacg cgctttagcg acaaatttgc
    13441 ctttggcaat gtccagttcg ataacacact tgcggaacac cgcatccggg cagaattgca
    13501 tcagatactg acgggcaatc aggttataga ccttcgcttc gttctccgtc aggttgatcg
    13561 cagaactccg tgcggtcgga atgatggcgt ggtgcgcatc gacctttttg tcatcccaac
    13621 agcggttgcg tatatctgga tctaccactg gctgcggcaa cagatccggt gcatgaacac
    13681 tgatggcatt catcaccgcg tggcgtccgg caaaatgttc ttctggcaaa tagcgacaat
    13741 cagaacgcgg ataagtgatt agcttgtgcg tttcgtacag tttctggcag atatcaagca
    13801 cgttctgcgc gctcagacca aagcgttttg ccgcttcaat ctgcaacgcc gaaagcgaaa
    13861 acggcagcgg cgcggattct gattcccgtt tatcgttata gctggtgaca atagccggtt
    13921 gaccactaat gcggttaacc acatgctccg ccagtggacg atgtaacaag cgcccttctt
    13981 catcctggta cggttcacac gcttcgctcg gttgccagat agcggtaaac cgctcatcgg
    14041 caggtgtcac gatatgcgct ttgacttcaa agaagtcttt cgccacgaag ttttcaatct
    14101 cttcatcgcg gcgcaccacc agcccgagca cgggtgtctg cacgcgcccc acggaaagca
    14161 cgccctgata accagcattg cgaccgagaa tggtgtaagc acgggtcata ttgatgccgt
    14221 acagccagtc ggcacgcgct cgcgccagcg cagaaacgca cagcggtaca aactcactgt
    14281 tggaacgaag acagtcgatc gcccgctcaa ccgcttgcgg gttcaggtcg tttatcaagc
    14341 aacgctgcac ctgctggcgc ttttccggtg ccagttgcag atagtccagc acttcatcca
    14401 ccagcaattg cccttcacga tccgggtccc cggcgtgaac gatttcgctg gcttcatgca
    14461 ggaagcgttt gatgacgtta agttgtttgg tcacggaggg tcggggctgt aattgccact
    14521 tttccgggac aatcggcaaa tccgcaagat tccagcgcgc atagcggctg tcgtaggcgt
    14581 ctggctgcgc ctgctcaagc aggtgaccga tacaccaggt caccacctga ccattaccgc
    14641 actcgataaa gccatcgcct ttccggtgcg gtttgggcag gacatcagca atggcgcgcg
    14701 ccagactcgg tttttcggca ataaacaacc gcattgaatt aacgaatctc aaccatggca
    14761 cgaccgccgc gcgctggcac cagttcgcca attgccgtca gttcaatgcc aaactcggcg
    14821 gctgtagctt tgacctcatt ttctgcttcc ggcatgaccg ccagcagcaa accgccagaa
    14881 gtttgcggat cgcacagcag atcgcgcact tcacgcggca tttcacccat cagatgaccg
    14941 tagctggcaa agttacgttc agtgccgcca ggtactgcgc ccaacttaat gtactcttca
    15001 acaccgggga gtttcgggat cgcttcatag tcgacgcgtg cctgcacacc agccccctga
    15061 cacatttcgc tcaagtggcc cagcagacca aagcccgtaa cgtcggtcat cgcttttacg
    15121 ccttcgatgt tggcaaagga cgcgcctgcg atgttcatcc ggcacatcac ttccgtcgcc
    15181 agtccctgat gttctggttt caacagtgat tttttctcag ccgtggtaag aacgccgatc
    15241 cccagcggtt tcgtcaggaa cagtttgcat ccggcttgtg cggtactgtt tttcttcacc
    15301 cgctcggtcg gtacgatccc cgttaccgcc agaccaaaaa tcggctccgg cgcatcgatg
    15361 gagtgaccgc cagccagcgc aatacccgcc tgacgacatg catagcgtcc accttcggtc
    15421 acttcgcggg caatttccgg ggaaagtttg ttaatcggcc agccgaggat cgcaatcgcc
    15481 ataatcggtt tgccccccat cgcgaagata tcgctgatgg cgttagtcgc cgcaatgcgg
    15541 ccaaaatcga aaggattatc aacgatcggc ataaagaagt cggtggtact gataacgctg
    15601 gtgccattgc ccagatcgta caccgccgca tcgtcgcggg tttcattacc cacaagcaaa
    15661 ttcggatcaa caaacttcgc ctgctcactg tgcaggatgg tttccaacac ttttggggaa
    15721 attttacagc cgcaaccagc tccgtggctg tattgggtca aacgaatcga gttctcgctc
    15781 atggacatct cctgtcaatg caatccgggt atggtaaccc tcattccgtg aagtgataag
    15841 tgagagtgtc tgaattcctg cgcctttgct cacaatccag acagtttcgc gacaattatc
    15901 agaaataagt tacaaacggc gtcgggtccg ggacgttaat cgacgtagat gctttcagct
    15961 gcggcgtacc gaggtagaga aaaccgacaa ttttatcctg ctcacggcaa ccgaatgctt
    16021 cacgcactac cggactttca gttaatgcgc cactgcgcca gatgccgcca aacccctggg
    16081 caactgctgc catttgcatc gccatgaccg cgcatccggc agacatttcc tgttcccagc
    16141 gcgggacttt atgattctct tcgcatttcg ccaccaccgt gatgatgagc ggtgcgcgga
    16201 acggcgcatt acgggctttg tcgatagctt tgtcatcact accggcagca atcgccccct
    16261 gttccagtac ggcgctgaaa cgctcgcgcc cttccccttc aatcacaaaa aaatgccacg
    16321 gttgcatgga cttatggtcc ggcgcacgca tacccgcacg caggatgttt tgcagttgtt
    16381 cacccgttgg cgcgggttca gccaagcggg aggcgctacg gcgattgatc aatagttcga
    16441 gtgcatccat ttgattaact cctgtcgtga tatttattca caaaattaac acgagagtgg
    16501 attttgttac agcacagtcc gcaattcctg ctgacaagta ccggttgggt cattacgata
    16561 accacatcta ttgcgcctgt gacaggtgtg accttaagtt gggagaatac atgcgaaccc
    16621 tttggcgatt tattgccgga ttttttaaat ggacgtggcg tctgctgaat ttcgtccgtg
    16681 aaatggtact taacctgttc tttattttcc tcgtactggt tggtgtgggg atttggatgc
    16741 aggtcagtgg tggtgattcg aaagaaacgg ccagtcgtgg cgcactgctg ctggacattt
    16801 ctggtgtgat cgtcgataaa cccgacagtt ctcagcggtt tagtaaatta agccgccagc
    16861 tgcttggtgc cagttccgat cgtctgcagg aaaactcact gtttgatatc gtcaacacta
    16921 ttcgccaggc gaaggacgac cgcaatatca ccggtattgt gatggatctg aaaaacttcg
    16981 caggcggcga ccaaccgtct atgcagtaca tcggcaaagc tctgaaagag tttcgtgaca
    17041 gcgggaaacc ggtttatgcc gttggcgaga actacagcca ggggcaatat tatctcgcca
    17101 gtttcgccaa taaaatttgg ctgtctccgc aaggcgtggt tgatctgcac ggctttgcca
    17161 ccaacggtct gtactacaaa tcgttgctgg ataagctgaa agtttccacc catgtgttcc
    17221 gcgtgggtac gtataaatct gccgttgaac cgtttattcg tgatgatatg tcaccggcag
    17281 cccgcgaagc tgacagccgc tggattggtg agctgtggca aaactatctg aatactgttg
    17341 ccgctaaccg gcagatccct gctgagcagg tattccctgg cgcgcaaggg ttgcttgagg
    17401 gtttaaccaa aaccggtggc gataccgcga aatatgcact ggaaaacaag ctggtcgatg
    17461 cactggcatc gagtgcggaa atcgaaaaag cactgaccaa agaattcggc tggagtaaga
    17521 ctgataaaaa ttatcgcgcc atcagttatt acgattacgc attgaaaacg ccggcagata
    17581 ccggtgacag catcggtgtc gtctttgcta atggcgcaat tatggatggc gaggaaactc
    17641 aggggaatgt tggcggtgat accactgcgg cacaaatccg cgacgctcgc cttgacccga
    17701 aagtgaaagc gattgtcctg cgtgttaata gcccaggcgg cagcgttacc gcgtctgaag
    17761 tgattcgcgc tgaactggca gcagcccggg cagcgggtaa gcctgtggtt gtatcgatgg
    17821 gcggcatggc ggcatctggt ggttactgga tttccacgcc agctaattac attgtggcta
    17881 accccagcac cctgaccggt tctatcggta tcttcggcgt gatcaccacc gtagaaaata
    17941 gtctggattc gattggtgtt catactgatg gtgtctcaac ttcaccgctg gcggatgttt
    18001 ctatcaccag ggcactgccg ccggaagcgc agcagatgat gcaattaagc attgagaatg
    18061 gctataaacg ctttatcacg ctggttgctg atgcgcgtca ttcgacgccg gagcaaattg
    18121 ataaaatcgc ccagggccac gtctggaccg gtcaggatgc aaaagctaac gggctggtcg
    18181 atagtctcgg ggatttcgat gatgcggttg ccaaagcagc agagctcgca aaagtgaaac
    18241 agtggcatct ggaatactac gttgatgaac cgaccttctt cgacaaagtg atggacaaca
    18301 tgtctggttc tgtccgggca atgttgccag atgcgttcca ggccatgtta cctgcaccgc
    18361 ttgcctcggt agcctctacc gttaaaagtg aaagcgacaa gctggccgcg tttaacgacc
    18421 cacaaaaccg ttatgcgttt tgcctgacct gcgccaacgt gcgttaagtc ttgtactgag
    18481 tggccgacag atcgtcggcc acattatttt ttacctcgac gaatcctctt cccgctgtct
    18541 cgccccatat ttccttatcc gcatagtatc aggtgcgctc ctcctgcctc acgtatatac
    18601 ttttgctctt tcgatatcat tcatatcaat atcatgcaaa agaaatcaat ttacgttgcc
    18661 tacacgggcg ggaccatcgg gatgcagcgt tccgagcagg gttatatacc ggtgtcaggt
    18721 catctacaac gccaactggc gctgatgccg gaattccatc gcccggagat gccagatttc
    18781 accattcatg aatatacgcc gctgatggat tcttctgata tgacgccaga agactggcag
    18841 catattgctg aagatattaa agcgcactat gacgactatg atggttttgt cattctgcac
    18901 ggcaccgaca cgatggcgta taccgcctct gcgctgtcgt tcatgctcga aaatctcggt
    18961 aaaccggtca ttgtgacagg gtcacaaatc ccgctggctg agttacgctc tgacggacaa
    19021 attaatctgc tgaatgcgtt gtacgttgcg gcgaattatc cgatcaacga agtaacgctc
    19081 ttttttaata atcgactgta tcgcggcaac cgcaccacca aagcccatgc cgatggtttt
    19141 gatgcgtttg cctctccaaa ccttcctccg ttactggaag caggtatcca tatacgtcgt
    19201 ttgaatacgc cacccgcccc gcacggtgaa ggggaattga tcgttcatcc aatcacgcca
    19261 caaccaattg gcgtagtgac gatttatccg gggatttctg ctgacgtcgt gcgcaatttt
    19321 ctgcgccaac cggtgaaagc attgattctg cgctcatatg gcgtgggtaa tgcgccacaa
    19381 aacaaagcct tcctgcagga attacaagaa gccagcgatc gcggtattgt ggtggtcaac
    19441 ctgacacaat gtatgtccgg taaagtgaac atgggtggtt atgccaccgg taacgccctc
    19501 gcccatgccg gcgttattgg cggtgcagat atgactgtag aagccacgct aaccaaactg
    19561 cattacctgc tgagccagga actggatact gaaaccattc gcaaggccat gagccaaaac
    19621 ctgcgcggcg aactgacgcc ggatgattaa ggagactgca atgccccctc gcgccctgtt
    19681 actggtcgat ttacaaaatg atttctgtgc tggtggcgcg ctcgccgtgc cggaaggtga
    19741 cagtacggtg gatgtcgcta accgcctgat tgactggtgc cagtcgcgcg gtgaagcggt
    19801 tatcgccagt caggactggc acccggcgaa tcacggcagt tttgccagtc agcacggtgt
    19861 cgagccttat acgccaggcc aactcgacgg tttgccacaa accttctggc cagatcactg
    19921 tgtgcagaac agtgaaggcg cacaattaca tccgttactg aaccaaaaag cgatcgcagc
    19981 ggtgttccat aaaggcgaaa atcctttagt tgacagttac agtgcctttt ttgataacgg
    20041 ccgtcggcag aaaacctcgc tcgatgactg gttgcgcgat catgaaatcg atgaattgat
    20101 cgttatgggc ctggctactg actattgcgt gaagtttacc gtgctggacg cgttacagtt
    20161 aggttataag gtgaacgtga ttaccgatgg ttgtcgtggc gtgaatatcc agccccagga
    20221 cagtgcgcac gcgtttatgg agatgtcagc agctggggca acgctatata cgctggcaga
    20281 ctgggaagag acgcaggggt aattttacgc tggcctacaa ttctgtactg gcattgtagg
    20341 ccaaataaaa cacgtcagtg gtacatctgg caattgatgc catcaacgaa agatttaatt
    20401 cacctcagaa atctcttcca acgacacctt ccgcgtttcg atgccaaaaa tggagagaac
    20461 cagcgcacag agcaataaca tgacaccaag caccataaac accgtgatcg acccataatg
    20521 tgttaataat gccgcaacgc catagggcgt gaaaactgcg acgatccgtc cgacggcatt
    20581 aacgaaaccc gaaccgcgca ggcgtaaatg cgttggccaa agctccggga tataaaccgc
    20641 cgacgcgaag caaacataca tgtataaaaa gaagatcatc accagaccat agattaaaat
    20701 cgcccactct gtagtctgaa ttgaatagat atagcctaac acggcaataa taataagtaa
    20761 ggcggagcca aataatcgac gaggaaaatg atcaataatt aatgccgcaa taaatattcc
    20821 taccggagcg ccaatcataa taacagcggt cattaatatt gatttatcga catcaatgcc
    20881 ggagttaaca aatatggtcg gtatccatac ggtgatggta taaagcgaaa tgttcattgc
    20941 aattaaaaca gtaatcgcga ctaatgtacg tcgtaacatt tcccctttaa acaggagcca
    21001 gaaggtaccc ttgattacct taaccttgct gttgctctgg ttcaaagtaa gctggggtaa
    21061 acgaatactc ttctctcttt caatttgctg ctctacttca cgaagttggc tttctgcacc
    21121 ggcgatttgc cctttccctg ccagccatcg tggcgactca ataaagtatt tacctgagag
    21181 aagccaggct aacagtatgc caataccccc caacagaaac attatccgcc aactaaaaaa
    21241 agcgataacc accacgccta tcgccgcaga tagcatgggc gaccagttac caacaaatga
    21301 gagccgcgcg gaccattttc cacgcaccgt cgcggggata aactccgtaa atgaggcata
    21361 gccaaccata atcagcgccc ccattcctgt tcccatgagg aagcgaaaga aaatgagcca
    21421 gtacatatca gggacaaaag cggcccctgt tgcagcaatt ccgacgatga gaagatttat
    21481 gcgaaacgcc ctgcgccgcc caaagtagtc accaataaac ccaccagtaa gtgagccgat
    21541 gaaataacca aacattaatg ccgaggtaaa ggcggcattg aggaaattat ttgaccatcc
    21601 attgcttacc agctttgcta agacgacatt acctgagtaa ctcaaaaacc ccgttaataa
    21661 cagactaaag cttattatac caaatatacg ataatgaaac cgggccaaag gcaagcggtc
    21721 cagtcttgcg ccaatttgat catattgttc cattgggtgc ctcggatttg ctaaaaaagg
    21781 tagcaattgc ggcaaattca cttgccgcat ctttatcatc attttatttc caggcgcagg
    21841 aacaaataat attgacaacg tgtgcttaaa ttaccacacc agctggatat tattgtcttt
    21901 gcaaaatgct atccactctg cacccggtga tttatcagtt attatgtaat taatatgact
    21961 aaagtcagct aactggacaa aagctttacg atcaaactta gagtgatcaa ccaataacac
    22021 aacttctgtc gcctgacgga tcattgtctt tttgatttca gcttccgctt cattagagtc
    22081 cagcgcgccg ctgttaatat caagaccttt acaactcatt accatgatat cgacatggta
    22141 gcggctgatg atctctttag taattcttcc ttgcagggaa agtgtatttt tgtttagttc
    22201 cccacccgtt gaaacgactt taatttctga ctgagccaga acatgaatcg cttccgccga
    22261 gttggttagc aacgtcaggt cactacggtc ctgtaacaat ttgagcaatt ccattacggt
    22321 actactggaa tcggctgcca tggtggtttt attgtcgata aagggtagtg ccttgcgtgc
    22381 aataagctgc ttctcttcat aaaacgatga agcgcgctta taaaaatgga tattctccgt
    22441 caacatcgct gtatttaaaa cagcaccacc ataggttctg gtcaaaaagc cttcatcttc
    22501 cagcttctca agatcgcggc gaatggtttc ttcggttacc tgaaaaatcc cactcaaatt
    22561 tgagactgtc acctttttat cgttggcaac catttgctta attgcctgaa tcctgtcttt
    22621 tgccgccacg attacacccc tgtatctttt tacatcacat tagcgcgatt atcgcataac
    22681 cgatgtttac tttcaaaata acctgtttga atcacagatt ttcatcacag ttttcacaga
    22741 aacagaggtg aatcgtgttg agtatttaac gctccagggc ctctgccatt tccctcatca
    22801 atgttgcgtc tgcatccgat aagttgatat tcagtgccgc gacattttcg cgtacctgtt
    22861 ccggtgcagt agccccacta agaatggaga ttaaatcact ctgttttaat atccacgcca
    22921 gtgccagagt gggaattgtg cactgataac gagcacaaag tggctgccac tgttcaagca
    22981 tatcaatcac tttcagcatg ttttcacgct ggaaccagac tttatttgcc cgagcgccgc
    23041 ccggaacgta atcacgagtg atggtgccgg tcaacaatcc ctgctctagc ggggaataaa
    23101 cctgaaccac aatgccatta tcacgacata gcggcagcag ttcgttttcc attgcccggt
    23161 cgaggatact gtatttcgcc tgaataatat ccagttcacc atattgcaga tactcgcgga
    23221 tatggtcagc atcgacgtta gcagcgccta tcgcgcgaat tttcccttcg gcttttaact
    23281 cattaagcac tgcgacagtt tcagctatcg gcgtaaaaaa tggcggcacc gactgccagt
    23341 gcgtcatgta gatatcgatg taatcaatac ccagacgttg caagctggct tctacctctt
    23401 cgcggataga ttccggggaa aggtttttat acaactgccg atcgccaact ttgttgaata
    23461 aacttccttt tcgttcccag acaatgccgc atttggtttc tactacaacc tgttcacggg
    23521 gcagtttttt taacgcctga ccgacgataa cttcactatt gccaaagtta tatcctggcg
    23581 cagtatcaat cagattaatg ccacaacgat gggcttcaag aatcgtatca atacatattt
    23641 gccgatcgag atcgccattc catgcaggac cgccgccaat ggcccatgtc cccaacccca
    23701 ttcgcgaaag cgtaatatcc gttgtgccta aaggtatctt tttcatttgc cttcattatc
    23761 cttcgtattc ttccagcaat tgttcgacca gctttctgtt ttttacgccg gtggtggcac
    23821 cgacgcttag aaccgagata gccgccgttg cattggcaaa gcgtgcgcat tcacgcagat
    23881 ttttgccttc taacagtgcc gcaataaaac ctgaagcaaa gttatcgccc gcgccaatgg
    23941 tgtcgatggc ggttattcct gcgaccgccg gcaccttcat cgtcatgtca ccacgcttga
    24001 taaagcagcc gtctttaccc gttttaatca ccaccgtttt tacgccgcac gcaagaaagc
    24061 agtcagcaat ttcatccagt gtctctttcc cggtgagtaa ttttgcctcg gcataattag
    24121 gaaacagata atcgacataa ctcaatgctt cgcaaatatc atccagcgtt tcattcaacc
    24181 gcggtttgat catatcggca cagataatca tctgccgggc tttagcttgc gtaaaaattt
    24241 ctgttagcgc tttaccatcc aatagtggac tgttgaaaat actggccagt gataataatt
    24301 ttgcctgaga aaaccgcgca aaatcaacat cgtcaatatt caatttccac agactgccat
    24361 tacggtttgt gacaaacgtc cgctcgccat cctccgtcac cagtccaacg ttaatagagg
    24421 tatctatgct gacgtcctgc ttcacgcttt gaatatcaat attctctttg cggcaatggt
    24481 cgagaataaa ctgccctgcg gcatctttac ctacacgact cattaatgct gtgcgatggc
    24541 ccagacgaga aataattgtt gcttcattta ttgcatcacc cccggtggtc attgcgattc
    24601 tttcaagagg ataagagtcc acatcaaaga tatttttact gaccggttgc aatggaatat
    24661 caacaatagc ggcacctata caaataacgt cgagattatc catatcatta ttccgctttg
    24721 ccatcagaac cgaacagttt aattttttcc aatgcccgtt cttttacagc tttacgtact
    24781 tcacgttcta aatgcaggaa aggctgatcc tggttttctt tcactgctac cattgcggcc
    24841 tgacagagct ccgtatgaat gttgattttc gcaataccca acgaaattgc agttttaatg
    24901 tcggcatcac tgatccccga tgcgccatgc aaaaccagcg gaacagaaac agcatcgcgt
    24961 acgcgtttga cgacctcaaa gttcaattgc ggctctgacg tataaacccc atgctggttg
    25021 ccgatggcga cagccaatga gtcacagccc gtacgttcaa caaattcagc cgcctggtca
    25081 ggatcggtat agtgatagcc cgccagcgct tcctcataaa cggtttcatt accgacatgc
    25141 cctaattccg cctctaccgg aatacccagc ggatggaaga aatcgacagc ctctttggtt
    25201 aaacgaatat tttcttcgaa atcaaatgca gaagcatcac gcattaatga attcatacca
    25261 tgagtccagg cgttatgaat aatctccata ctccgaccat gatcccaatg agttattacc
    25321 ggcaccgttg ctttttgtgc cattgatacc atcatgtgag agaaatcttc aaatgaggtg
    25381 ttaccgacaa aacctgtacc aaaagaaata ataaccggtg atttcgcttc ttcggctgcg
    25441 tcgataacgc ccatcaacat ttctgcattc catacgttaa aatgggcaat tgcataatgt
    25501 ttatttgtgg catcgttttc ccaatatctg atatctgcga gcatattctg ttctcccgta
    25561 ttaattaatc gctaatttta ataacgcctt taataatgtc gcgtttgttg ttaactgact
    25621 cttcaaatgc ctgttgtaca tcccgataat cgtaaatatg cgtcaccatc gatttcacat
    25681 cgaatcgccc tgaagaaata gcttcaatcg tgaccggata acgattggca tagcggaata
    25741 ccgtctggat agtgacttcg cgattgattt tgaggaaatt gattgccgaa tcgcccggta
    25801 cagtaccaac aatcataatt ttaccgccgc gcattaccag ataaggtgcc tgtttaacgg
    25861 tgaccgcaga acccgctgtt tcgaaaacaa tatctgcgcc catgtcttcg gtaaattgct
    25921 gacagcgtgc aatagtgtct tcttttgcgc cgttaataac cactgtcgca ccaagttgtt
    25981 ccgccattat cagacgtttt tccagcacat caacgacggc aatttccgtt gctcccaggc
    26041 atttgcacgc ttgcaacgtc atcaaaccaa tacaacctgc tcccagaata attatcttct
    26101 tacccggttt aacatctgcc agcatcgcgg catgcatccc gactgcggca ggctccacca
    26161 gcgccccttc catcgtgtcc atattgtctg gcagtttgta agtaaagctc tccggatgac
    26221 acagatagtg cgttaatgcg ccgcggtagt tgggttgtgt cgccataaaa tcaacgtccg
    26281 ggcagatgtt atatttgcct tccagacagt aacgacagtg accgcaagga acgccaggtt
    26341 cgatatttac ccgatccccc ggtttaaatt tgcgcacgcg gcttcccaca gccacaaccg
    26401 tcccggcgca ttcatgaccc aggccaattt cttgatttgg gtctttaggc ggaataaacg
    26461 ggcctgattc aaaaccatgt acatctgaac cacaaatacc gacatattct actttaatca
    26521 aaacttcatc ttctttaggc actggtattt ctgctgaaat aattttcatt gtgcccggca
    26581 cctgcaatat tgcttttgaa tttttcattt tgaatccacc cgtatcattt attggctact
    26641 gcatcaatac ttaaactttc aacggagaca cctttggttt caattccaat ggtggcgata
    26701 gcaattgcga caacaattga aacagccccc agaagaataa agactcccgt tacgccataa
    26761 ctactgagca gcactgcaac ggcataaggt gcggcaatac cactgattcg ccctaccgca
    26821 ttcgccagac cggagccacg gagtttggcc tctgtcggcc agatttcagg gacatacact
    26881 gccgaggcat agcaaacgta catatagacg aaagtaatca ggaagaaacc aatcaacgtt
    26941 atgagcaaca tactggtttg cagtgaatag atatatccga gcaccgcaat caggattaat
    27001 agccccacac ccattgtttt acgcggaatt ttatccatca ccagcatggc aataaaaata
    27061 ccaaatggcg cgccaaacat actcatggta tttaaaacaa tcgagtcttt taaattaatc
    27121 ccctgggtca tgaatattgt tggcaaccag ttaattagtg tgtactgcac aacgttcatg
    27181 gcaatcagca cacaagagcc taatatcacg cgtttcagta atactcctgt cagtaaggct
    27241 gaatacggca ccgcttgtgg cgctttaccg tcatcagcaa taaccacagg cggcaaaggt
    27301 ttacccgtct ggcgtatgac gccttcttct atactccgca tgactttctc ggcttcctga
    27361 taccgtccgc gcgattccag ccagcgcggg gattcaggaa agtagcgcca ggccagcgcc
    27421 gtagcgataa gcgacaatat tgcagggata agcagttgta cccgccagtt ccactcagca
    27481 ctaatcagcg gcgtgagtcc catcgcaatc aatgaacaga gcggatatga ccagttgcca
    27541 ataaaggaaa cccgactgga ccacgttccg cgatttctac cgggcatata ttcggtgaaa
    27601 ccagcaaaca gcgtaaccag taaagctccc agccaaacgc ccatcacaaa acggcaggca
    27661 ataagaaaat ccatattcgg tgagaaagca ccgacaacca tcgaggcaat atgaatggcc
    27721 tcatagagga tgaacgcatt tctacgaccg gttttgtcgc caatgatgcc gcccaccagc
    27781 gcaccgagaa acattccggc ggtcgtgatt gctgagaatg tggctgtggt ggaattatct
    27841 gtccagccca acgctttcag ctgcgcgagg atcaagccac caacggcatt actccagcag
    27901 acaagcaagc caaacgcgac gatggcaaac attgacgaat gccagcggca atcaggtaag
    27961 cgatccagcc gtgcaccaca atgcggtttt gttatctgtt ccatgcgata acgttccttc
    28021 aggagagtgg ttattcatca aagtcgtaag tcatgatcac tttgattgcg gttttatcga
    28081 ccatcgcatc aaacccttcg cgccattgcg acaggccgat acgatgcgtg atcatcggtt
    28141 tgactttgat agcgccgctg gctaatagcc tgatagcgtt acgccatgag gtggagtcat
    28201 aggccatatg cccaatgatg cttttattcc aggcggtaat gtcattaatc gagaaatcaa
    28261 gaggtttgaa gcccattcca acgcgtacca cttccccatt cgggcggagc atttcgatgg
    28321 cttgtttcag tgcgatattg gcaccggagc attcaatcac cagtcccaga ttatctttgc
    28381 cacaaatttg ctggcagcgc gccaccacat cttcggtaga accatttact actgccgtcg
    28441 cccccagttc ttttgcaacc gggaagcgga ccgccacatc ttcttgcaga ccaacgacga
    28501 cgatatttac cgcccccata atccgcgcca tttgtacgga aaacagcccg agtgggccag
    28561 tgccgatgac gacaacatcc tgaccaggaa ggaatttcga ttgctgcgcg atggatttat
    28621 aggcattaca gatagggtca agtacggctg cgtcctcata atcaacacca tcagggattt
    28681 cccacaacgc atgacgatga attttgagaa tttcaccagg aaccagacaa tatttggaaa
    28741 aaccaccgcc ccaggtattg ttatccagac caagttttac cttttctgta caacacagaa
    28801 aatcaccctg ttcacaggcc ggacaaacgc cgcaaacgtg accgctgtta tccgacacga
    28861 cgcgttgccc cactttccag tctttgactt tttcaccaac ctgcgcaata caacctgcga
    28921 actcatggcc gcggatagag ttaaactcat cagaaccgct atcgacattg tagtgcttca
    28981 tgtctgcgcc gcagattgcc gcggctttaa tttcaatcac tacgtcttcc gggccacaaa
    29041 tgggttgtgg gacatcaatc atcttgtagc cgccaaaggc cttgccaaac cgagccagtg
    29101 ctttcattgc gtcatcctct gtgttttgtt ttgcttagat ggccatcata cttacccaca
    29161 tgatttttga ggtcaacatt aaatctgtaa aacacagata atgatctgcg ttttacaact
    29221 tagatcacaa ttacgcagaa aatggctcgg tctgttacag gttgatggaa ggcgggacgc
    29281 aaaaagagca aattcgagag ggattaccgg ccaaataggt ctgagactta agttcaggag
    29341 aagtcattca ggcgtgaacg cgacggcaag agatcggact gggacttagc caggtcgggg
    29401 cgagataatt gagacgagtc gtggggtcat tgttggctat ccccttcata gccgcggcaa
    29461 aaaggggata aaccacttac ttaaacatag caataggctt tgctgagata ccaaaatctt
    29521 cttttaactg ctgtttgctc ttcatgacca tctgaccatt tgtgtcaatt gtcatatgct
    29581 gtgcttcggt attatggcgg gcttgccaca gcatcaccag ttgcaggcaa ttctcttttt
    29641 gttcctcggt caacgcaacg ccatcaggcc atttccccag ttcaacggcg gtcgacaaac
    29701 gctggtatac ctcaggcgtc atgctgttga taatgtcatc aagattcata atttttccgc
    29761 tcctgtggaa taagttgctg aatcgttttt tcaaccgttg atttcttcgc cgttttcgcc
    29821 atcagtgaag cgtaaagaag cagagttaac acaataacgt tcgcctgttg gttgcggccc
    29881 gtcgggaaag acatgcccca gatgagcatc acagttaccg caacgaattt ctatgcgctg
    29941 cattccatgt gacaagtctt tgatataacg aatggattct tcactcaccg gttcgtagaa
    30001 actgggccag ccacagccgg aatcatactt ggtttcggaa tgaaacagcg gggcatcgca
    30061 gatcaaacag tgatatacgc cgtcacgctt gttatgcagt aaacgacccg taaatggcgg
    30121 ttctgtccca tgattctgcg tcacgtaaaa ctgcatctcg gacaaatttt ttttcagttc
    30181 ttctgccgaa ggtttattag ccatttgctc acatctcact ttaatcgtgc tcacattacg
    30241 tgactgattc taacaaaaca ttaacaccaa ctggcaaaat tttgtcctaa acttgatctc
    30301 gacgaaatgg ctgcacctaa atcgtgatga aaatcacatt tttatcgtaa ttgcccttta
    30361 aaattcgggg cgccgacccc atgtggtctc aagcccaaag gaagagtgag gcgagtcagt
    30421 cgcgtaatgc ttaggcacag gattgatttg tcgcaatgat tgacacgatt ccgcttgacg
    30481 ctgcgtaagg tttttgtaat tttacaggca accttttatt cactaacaaa tagctggtgg
    30541 aatatatgac tatcaaagta ggtatcaacg gttttggccg tatcggtcgc attgttttcc
    30601 gtgctgctca gaaacgttct gacatcgaga tcgttgcaat caacgacctg ttagacgctg
    30661 attacatggc atacatgctg aaatatgact ccactcacgg ccgtttcgac ggtaccgttg
    30721 aagtgaaaga cggtcatctg atcgttaacg gtaaaaaaat ccgtgttacc gctgaacgtg
    30781 atccggctaa cctgaaatgg gacgaagttg gtgttgacgt tgtcgctgaa gcaactggtc
    30841 tgttcctgac tgacgaaact gctcgtaaac acatcaccgc tggtgcgaag aaagtggtta
    30901 tgactggtcc gtctaaagac aacactccga tgttcgttaa aggcgctaac ttcgacaaat
    30961 atgctggcca ggacatcgtt tccaacgctt cctgcaccac caactgcctg gctccgctgg
    31021 ctaaagttat caacgataac ttcggcatca tcgaaggtct gatgaccacc gttcacgcta
    31081 ctaccgctac tcagaaaacc gttgatggcc cgtctcacaa agactggcgc ggcggccgcg
    31141 gcgcttccca gaacatcatc ccgtcctcta ccggtgctgc taaagctgta ggtaaagtac
    31201 tgccagaact gaatggcaaa ctgactggta tggcgttccg cgttccgacc ccgaacgtat
    31261 ccgtagttga cctgaccgtt cgtctggaaa aagctgcaac ttacgagcag atcaaagctg
    31321 ccgttaaagc tgctgctgaa ggcgaaatga aaggcgttct gggctacacc gaagatgacg
    31381 tagtatctac cgatttcaac ggcgaagttt gcacttccgt gttcgatgct aaagctggta
    31441 tcgctctgaa cgacaacttc gtgaaactgg tatcctggta cgacaacgaa accggttact
    31501 ccaacaaagt tctggacctg atcgctcaca tctccaaata agttgagatg acactgtgat
    31561 ctaaaaagag cgacttcggt cgctcttttt tttacctgat aaaatgaagt taaaggactg
    31621 cgtcatgatt aagaaaattt ttgcccttcc ggtcatcgaa caaatctccc ctgtcctctc
    31681 ccgtcgtaaa ctggatgaac tggacctcat tgtggtcgat catccccagg taaaagcctc
    31741 ttttgcatta cagggcgcac accttctctc gtggaaacct gcgggtgaag aagaagttct
    31801 gtggttgagc aacaacacac cgttcaaaaa tggcgtcgct attcgcggtg gcgtaccggt
    31861 ttgctggccg tggtttggtc cggcggcaca acaaggtctg cctgcgcacg gttttgcccg
    31921 caacctgccg tggacgctga aatcacatca tgaagatgct gatggcgtag cgctgacttt
    31981 tgaattgacg caaagcgaag agacgaaaaa attctggccg cacgacttta cgctgttagc
    32041 gcatttccgc gtgggtaaaa cttgtgaaat cgatcttgaa tcacatggcg aatttgaaac
    32101 cacctctgcc ctgcatacct actttaacgt gggtgatatc gctaaggtaa gcgtcagtgg
    32161 gctgggcgat cgcttcattg ataaagtgaa tgacgcgaaa gaaaatgtac tgaccgatgg
    32221 tattcagacc ttccctgacc gtaccgatcg cgtgtatctg aatccacaag attgcagcgt
    32281 gattaatgat gaagcgctga atcgtattat cgccgtaggc caccagcatc atctgaacgt
    32341 tgtcggctgg aacccgggac cggcgctttc aattagcatg ggcgatatgc cggatgatgg
    32401 ctacaaaaca tttgtttgtg tagaaacggc ttacgcttca gaaacgcaaa aagtgaccaa
    32461 agagaaacct gcacatctgg cgcaatccat tcgcgttgcg aaacgttaat ttacgttaat
    32521 gttgtgtgcc gggtgcgatg catccggcac acaacatcac accatatcca gcgcagtttt
    32581 tccttttggt gccggatatg ccttatccag catagctaat tccgctgaag aaagttcgac
    32641 ctcaagcaca gccgcatttt gttggacatg ggcaatcgtg gccgcttttg gaatcgccat
    32701 cacaccctga tgactgatca cccacgccaa caatacttgt gccgcgctga tattgtgagc
    32761 atgtgcaatt tcgttgacta ccgcgttttt taacagtcca ttgcgcaacc gcccggcctg
    32821 ggctaacgga ctgtaagcca tcaccggcat ctgctgttgc tggcaccagg ggagtagatc
    32881 gtactcaatt cctcgtgaac cgagatggta aagcacctga ttagtggcac actgatttcc
    32941 ccccggcagc tgccagagtt cctgcatatc agcataatca aggttagaaa cgccccagcg
    33001 gcggattttt ccctgggcga tcaatttttc catcgctgcg acagtctctt caaaagcgaa
    33061 actgccagac cagtgtaata agtaaagatc gagataatca gtattgagac ggcgtaaact
    33121 ggcttcgcat gcatttatcg ctttttgccc gccagcattc cacggataga ctttagagac
    33181 gagaaagacc ttctctcgca gaccggttaa tgcttcccca accacctttt cggcaccgcc
    33241 atcggcatac atttcggcgg tatcaatgag ggttaaaccg agttcaatgc ccgcgcgtag
    33301 tgcagcaact tctgttttgc gctgactggc atcttcgccc atataccatg ttccctgccc
    33361 tacggctggc agtgagacat cgccactaaa ttgaatcatt ttttgttgca ttgtttcctc
    33421 ccaggtattg caccaccgta atgcaaaaca gggcgtgacg ccctgttttt atgcacaaaa
    33481 tgccctggaa agatgcatta tcagaatttg taggtgatcc cggtagaaat caggccagtc
    33541 caggatttat ccaccatcgg gctgtcagta acttcatcag acagacgggt gtagcgtgcg
    33601 gtaccgtaaa cactccagtc gccgaggaag ttgtagctgg cgctcagctc caggtaaggg
    33661 ctccagctgt cgttcgggtt atagccacgc agaccgctgc gagcggactc tttgcgcgat
    33721 acgccataat agtattcgtt ctggttttcg ctgttccact gcacaccaat acccggagtc
    33781 acggtcaggc caccgttggt gtaacgatac aaccaggcca tatcccagac gatgccgttg
    33841 ctgttatcca gggtatcgcc agccagggtg gtacgcaggt aaccgtactg ggtaaagtga
    33901 gcataagaca gaccagccat catggtgctc ttacggtcat ccaggtgacg catttggtga
    33961 tcgccactgt ctttcgcttt gaagtaaagc ggcgaccagt aagcggtaat tgaaagttta
    34021 tccgttgcgt cattccacag gtagtaacca ccacctaagc cacggaacca gaagttatcg
    34081 ccttcatagt tgattaccgg tactgggtaa acatcggtat cgtaatcttt atatgggtgt
    34141 tcaacgacac ctacgcctgc gcccagggaa aatttacctt cagcgtgcgc tacgcctgca
    34201 gacgttgcga taagcactcc aagtgccaga agtttgagtt tggtcacaat taatcattcc
    34261 ttaaacaaat gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa cccaaccttt
    34321 ttacgttttc attttttaaa gtaactgttt aattttcctg acgcggatga caccgcgctt
    34381 acagccaaat gaatttagcg ttactggcga gcctggtctt tacattaatt atgcaaaatt
    34441 tatggatgag ttgttgatat gccattgaaa ttaagaaagc cgtgcaggca agttttccat
    34501 ttgccatcta cgcttaattt tgaaggtgta tcaccgggca cgttgttctc atcgtcgata
    34561 aaatggcatg agagttgctg tgttttagca agagacgtcg ttcagtttac ctcttccggg
    34621 agcctctact attcatatga acggctctta acctgtgcta aaaaacgaaa ggacggcata
    34681 ccatgaatat attcgatcac tatcgccagc gatatgaagc tgccaaggac gaagagttca
    34741 cactgcagga gtttcttacc acttgtcggc aagatcgcag tgcttatgcc aacgcggctg
    34801 agcggctatt gatggctatc ggtgagcctg tcatggtcga tacagcccag gaacccagac
    34861 tttctcgact cttttctaac cgggtcattg cacgttatcc ggcgtttgaa gagttttacg
    34921 gcatggaaga cgcgattgaa cagattgtct cttatctgaa acacgcggct caggggctgg
    34981 aagagaagaa acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa tcatcgcttg
    35041 ctgagcgact gaaatcatta atgcagctcg taccgattta tgtattgagc gcgaacggtg
    35101 agcgtagccc ggtcaacgat catccgttct gtcttttcaa tccgcaggaa gatgcgcaga
    35161 ttctggaaaa agagtatggc attcctcgcc gttatctcgg caccatcatg tcgccgtggg
    35221 cggcaaaacg cctgcatgaa tttggtggcg atatcactaa gttccgggta gtgaaggtct
    35281 ggccgtcaat tctgcaacaa attgctatcg ccaaaacgga acccggcgat gagaacaacc
    35341 aggacatctc cgcgctggtt gggaaagtcg atattcgtaa actcgaacac tacgcgcaga
    35401 atgacccgga cgcctacggc tattccggtg cgctgtgccg cgccaatcag gggatcatgg
    35461 aattcgttga gatgtttaaa gcaccgatta aagtgctgca tcccttgtta accgccactc
    35521 aggaaggtaa ctacaacggg acggaaggta tctccgccct gccgttcaac gggattattc
    35581 tcgcacactc gaacgagtcc gaatgggtca ctttccgtaa taacaaaaac aacgaagcct
    35641 tcctcgatcg tgtttacatc gtgaaggtgc cgtattgctt gcgcatttcc gaagagatca
    35701 aaatctacga gaaattgctt aatcacagtg aattgactca tgccccatgc gcccctggca
    35761 cgctcgaaac actgtcacgt ttttccattc tttcgcgcct gaaagagcca gaaaactcca
    35821 gcatttattc aaagatgcgg gtttatgatg gcgaaagtct gaaagacacc gatcccaaag
    35881 ccaagtcgta tcaggaatat cgtgactacg ccggtgtcga tgaagggatg aacggtctgt
    35941 cgacgcgttt tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat gtagaagtgg
    36001 cagcaaaccc ggtccatctg ttctacgtcc tggaacagca gattgagcgc gagcagttcc
    36061 cacaagagca ggcagaacgc tatctggagt tcctgaaagg ttatctgatc ccgaaatatg
    36121 ccgagtttat cggcaaagag atccagacgg cctaccttga atcctattcc gaatatgggc
    36181 aaaacatttt cgaccgttat gttacctacg cggatttctg gattcaggat caggagtatc
    36241 gcgatccgga taccgggcag ctgtttgacc gcgagtctct taacgccgag ctggagaaaa
    36301 tcgagaaacc ggcggggatc agtaatccaa aagatttccg caacgagatt gttaacttcg
    36361 tactgcgcgc cagagcgaat aacagcggac gcaatccgaa ctggaccagc tatgaaaaac
    36421 tgcgcacggt catcgagaag aaaatgttct ccaataccga ggagctgttg ccggttatct
    36481 cgtttaacgc caaaacgtca accgacgagc agaagaaaca cgacgacttt gtcgaccgta
    36541 tgatggaaaa aggctacacc cgtaaacagg tgcgtttact gtgcgaatgg tatttgcgcg
    36601 tacgtaaatc gtcttaacaa ccctggcccg gtacacatgt taccgggcct acaacgacag
    36661 cgaaccgtgg gcctgagaag cggcaacaca ggcgtagcat acagttggca aatgtagtac
    36721 ggggggcata tgacctggtt tattgaccgg cgtctgaacg gcaaaaacaa aagcatggtg
    36781 aatcgccagc gttttttacg ccgttataaa gcgcaaatta aacagtcgat ctccgaggcc
    36841 attaataagc gttcggtgac tgacgtcgac agcggcgaat ccgtatccat tcccacggaa
    36901 gatattagcg aaccgatgtt tcatcagggg cgtggcggtc tgcgccaccg cgtgcatccg
    36961 ggcaatgacc atttcgtcca gaacgaccga attgaacgtc cccagggtgg cggcggaggt
    37021 tccggcagtg gtcagggcca ggccagccag gatggtgaag gtcaggatga atttgtcttt
    37081 cagatttcga aagatgagta tcttgatctg ctctttgaag atttggcctt accgaatctg
    37141 aaacaaaacc aacaacgcca gctgaccgaa tataaaacgc atcgggcggg ttataccgct
    37201 aacggcgttc cggccaatat cagcgttgtg cgttcattgc agaactcact ggcgcgacgc
    37261 acagccatga cggcaggcaa gcggcgggaa cttcatgcac tggaagagaa tttggccatc
    37321 atcagcaaca gtgaacctgc gcaactgctg gaagaggaac gtctgcgcaa agaaattgca
    37381 gaattacgtg ccaaaattga acgcgtccct tttattgaca ccttcgattt acgttacaag
    37441 aactacgaga agcggcccga tccctccagc caggcagtga tgttttgcct gatggacgtt
    37501 tccggttcaa tggatcaatc cactaaagat atggctaagc gtttttatat tctgctgtat
    37561 ctgttcctca gcagaacgta taagaacgtg gaagtcgtat acatccgcca tcatacccag
    37621 gcgaaagaag tcgatgaaca tgagtttttc tactcgcagg aaacaggcgg caccattgtt
    37681 tccagcgccc tgaaactgat ggatgaggta gtgaaagagc gttataaccc ggcacagtgg
    37741 aatatttacg ctgcacaagc atcggacggc gataactggg ccgatgactc tccgctttgc
    37801 catgaaatcc tggcgaaaaa attattacct gttgttcgtt attacagcta tatcgaaatt
    37861 acccgtcgtg cacatcagac attgtggcga gaatatgagc atctgcaatc tactttcgac
    37921 aactttgcga tgcagcacat ccgcgaccag gatgatattt atccggtgtt ccgtgaactg
    37981 tttcataaac aaaatgcaac agctaaagac taaaactatc agccaggtca ttatcgcctg
    38041 gctgattttt agcttactgt aaattatctc atttattaca
//
LOCUS       DAPHBJ010000020        37780 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.020, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000020 DAPHBJ010000000
VERSION     DAPHBJ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.020"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            191..352
                     /locus_tag="RHZ76_000953"
     CDS             191..352
                     /locus_tag="RHZ76_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2811638.1"
                     /translation="MEISFSPKRLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(436..1671)
                     /locus_tag="RHZ76_000954"
     CDS             complement(436..1671)
                     /locus_tag="RHZ76_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDV2811639.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAEHHFLVGLSLDGPPEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAAREGLKLH
                     APGDIQGELTEWSVRPEEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMHAHLLVVSK"
     gene            complement(1818..1894)
                     /locus_tag="RHZ76_000955"
     tRNA            complement(1818..1894)
                     /locus_tag="RHZ76_000955"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1858..1860),aa:Pro,seq:tgg)
     gene            complement(1937..2023)
                     /locus_tag="RHZ76_000956"
     tRNA            complement(1937..2023)
                     /locus_tag="RHZ76_000956"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1987..1989),aa:Leu,seq:cag)
     gene            complement(2044..2119)
                     /locus_tag="RHZ76_000957"
     tRNA            complement(2044..2119)
                     /locus_tag="RHZ76_000957"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2084..2086),aa:His,seq:gtg)
     gene            complement(2178..2254)
                     /locus_tag="RHZ76_000958"
     tRNA            complement(2178..2254)
                     /locus_tag="RHZ76_000958"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2218..2220),aa:Arg,seq:ccg)
     gene            complement(2357..3742)
                     /locus_tag="RHZ76_000959"
     CDS             complement(2357..3742)
                     /locus_tag="RHZ76_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV2811640.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3933..4673)
                     /gene="rffM"
                     /locus_tag="RHZ76_000960"
     CDS             complement(3933..4673)
                     /gene="rffM"
                     /locus_tag="RHZ76_000960"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HDV2811641.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGHLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4676..6028)
                     /gene="wzyE"
                     /locus_tag="RHZ76_000961"
     CDS             complement(4676..6028)
                     /gene="wzyE"
                     /locus_tag="RHZ76_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HDV2811642.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFHRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMFAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(6025..7104)
                     /gene="rffT"
                     /locus_tag="RHZ76_000962"
                     /gene_synonym="wecF"
     CDS             complement(6025..7104)
                     /gene="rffT"
                     /locus_tag="RHZ76_000962"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HDV2811643.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(7101..8351)
                     /gene="wzxE"
                     /locus_tag="RHZ76_000963"
     CDS             complement(7101..8351)
                     /gene="wzxE"
                     /locus_tag="RHZ76_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HDV2811644.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8353..9483)
                     /gene="rffA"
                     /locus_tag="RHZ76_000964"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8353..9483)
                     /gene="rffA"
                     /locus_tag="RHZ76_000964"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HDV2811645.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9488..10162)
                     /gene="rffC"
                     /locus_tag="RHZ76_000965"
                     /gene_synonym="wecD"
     CDS             complement(9488..10162)
                     /gene="rffC"
                     /locus_tag="RHZ76_000965"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HDV2811646.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10140..11021)
                     /gene="rfbA"
                     /locus_tag="RHZ76_000966"
     CDS             complement(10140..11021)
                     /gene="rfbA"
                     /locus_tag="RHZ76_000966"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDV2811647.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(11040..12107)
                     /gene="rffG"
                     /locus_tag="RHZ76_000967"
     CDS             complement(11040..12107)
                     /gene="rffG"
                     /locus_tag="RHZ76_000967"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDV2811648.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(12107..13369)
                     /gene="wecC"
                     /locus_tag="RHZ76_000968"
     CDS             complement(12107..13369)
                     /gene="wecC"
                     /locus_tag="RHZ76_000968"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDV2811649.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13366..14496)
                     /gene="wecB"
                     /locus_tag="RHZ76_000969"
     CDS             complement(13366..14496)
                     /gene="wecB"
                     /locus_tag="RHZ76_000969"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDV2811650.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14552..15598)
                     /gene="wzzE"
                     /locus_tag="RHZ76_000970"
     CDS             complement(14552..15598)
                     /gene="wzzE"
                     /locus_tag="RHZ76_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HDV2811651.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMAAADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYTQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15610..16713)
                     /gene="wecA"
                     /locus_tag="RHZ76_000971"
     CDS             complement(15610..16713)
                     /gene="wecA"
                     /locus_tag="RHZ76_000971"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HDV2811652.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16953..18212)
                     /gene="rho"
                     /locus_tag="RHZ76_000972"
     CDS             complement(16953..18212)
                     /gene="rho"
                     /locus_tag="RHZ76_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDV2811653.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18297..18398)
                     /gene="rhoL"
                     /locus_tag="RHZ76_000973"
     CDS             complement(18297..18398)
                     /gene="rhoL"
                     /locus_tag="RHZ76_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HDV2811654.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18539..18868)
                     /gene="trxA"
                     /locus_tag="RHZ76_000974"
     CDS             complement(18539..18868)
                     /gene="trxA"
                     /locus_tag="RHZ76_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HDV2811655.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18999..20264
                     /gene="rhlB"
                     /locus_tag="RHZ76_000975"
     CDS             18999..20264
                     /gene="rhlB"
                     /locus_tag="RHZ76_000975"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HDV2811656.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20400..21884
                     /gene="gppA"
                     /locus_tag="RHZ76_000976"
     CDS             20400..21884
                     /gene="gppA"
                     /locus_tag="RHZ76_000976"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HDV2811657.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(21931..23952)
                     /gene="rep"
                     /locus_tag="RHZ76_000977"
     CDS             complement(21931..23952)
                     /gene="rep"
                     /locus_tag="RHZ76_000977"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HDV2811658.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGAELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24169..24617
                     /locus_tag="RHZ76_000978"
                     /pseudo
     CDS             24169..24617
                     /locus_tag="RHZ76_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            24816..25097
                     /gene="ppiC"
                     /locus_tag="RHZ76_000979"
     CDS             24816..25097
                     /gene="ppiC"
                     /locus_tag="RHZ76_000979"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HDV2811659.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25184..26659)
                     /gene="ilvC"
                     /locus_tag="RHZ76_000980"
     CDS             complement(25184..26659)
                     /gene="ilvC"
                     /locus_tag="RHZ76_000980"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDV2811660.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     INLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNGQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            26809..27702
                     /gene="ilvY"
                     /locus_tag="RHZ76_000981"
     CDS             26809..27702
                     /gene="ilvY"
                     /locus_tag="RHZ76_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HDV2811661.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNHK"
     gene            complement(27754..29298)
                     /gene="ilvA"
                     /locus_tag="RHZ76_000982"
     CDS             complement(27754..29298)
                     /gene="ilvA"
                     /locus_tag="RHZ76_000982"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HDV2811662.1"
                     /translation="MADSQPLSGTPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIALHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTYWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29301..31151)
                     /gene="ilvD"
                     /locus_tag="RHZ76_000983"
     CDS             complement(29301..31151)
                     /gene="ilvD"
                     /locus_tag="RHZ76_000983"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDV2811663.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDRII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31216..32145)
                     /gene="ilvE"
                     /locus_tag="RHZ76_000984"
     CDS             complement(31216..32145)
                     /gene="ilvE"
                     /locus_tag="RHZ76_000984"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HDV2811664.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32165..32428)
                     /gene="ilvM"
                     /locus_tag="RHZ76_000985"
     CDS             complement(32165..32428)
                     /gene="ilvM"
                     /locus_tag="RHZ76_000985"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HDV2811665.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(32425..34071)
                     /locus_tag="RHZ76_000986"
     CDS             complement(32425..34071)
                     /locus_tag="RHZ76_000986"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HDV2811666.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     NTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(34074..34124)
                     /locus_tag="RHZ76_000987"
     CDS             complement(34074..34124)
                     /locus_tag="RHZ76_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2811667.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34211..34309)
                     /gene="ilvL"
                     /locus_tag="RHZ76_000988"
     CDS             complement(34211..34309)
                     /gene="ilvL"
                     /locus_tag="RHZ76_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HDV2811668.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            34662..36182
                     /locus_tag="RHZ76_000989"
     CDS             34662..36182
                     /locus_tag="RHZ76_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDV2811669.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEHGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(36207..36545)
                     /gene="maoP"
                     /locus_tag="RHZ76_000990"
     CDS             complement(36207..36545)
                     /gene="maoP"
                     /locus_tag="RHZ76_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HDV2811670.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            36664..37503
                     /gene="hdfR"
                     /locus_tag="RHZ76_000991"
     CDS             36664..37503
                     /gene="hdfR"
                     /locus_tag="RHZ76_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HDV2811671.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(37599..37674)
                     /locus_tag="RHZ76_000992"
     tRNA            complement(37599..37674)
                     /locus_tag="RHZ76_000992"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37639..37641),aa:Trp,seq:cca)
     gene            complement(37683..37759)
                     /locus_tag="RHZ76_000993"
     tRNA            complement(37683..37759)
                     /locus_tag="RHZ76_000993"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37723..37725),aa:Asp,seq:gtc)
BASE COUNT         9241 a        10302 c         9506 g         8731 t
ORIGIN      
        1 ccttacatat caactgcgcc agaggtagga ttgaaaacgc tctcctgatt ttccaattca
       61 ttttctggat aaataaataa tttatttttg tcactattat ttatgtaatc atcctgtcag
      121 ggagagggat ctcaattatc aatgcttaat tacgtcatca ttttgatgac atgaaaccgg
      181 agcattttgc atggaaattt cgttttcacc caaacgtctt gttgttgctg tcgccgccgc
      241 tcttcctctc atggccagcg cagcagatac cccgtcaact gccaccgcac gcaaaggctt
      301 tgccggatac gatcacccaa accagtatcc acagaacgct gcaatttatt gaatttgcac
      361 gatcatgtag gccggataag gcgtttacgc cgcatccggc aatcaaccgc aggcggccac
      421 aaaatggccg cttttttact tactcaccac cagcaaatgc gcatgcataa tgtcactggc
      481 cgggcgaccg tgcgccagca aatcagccat tgctttaaga tatggcggta gatggcggaa
      541 ataacgctga tacccggcac acaaataatt cagccccggt ttgccgctgg catcgagcat
      601 gaagcggtgt ttcgggcagc ctccccagca cgcttttaac acgttacaac tgcgacactg
      661 cgccggtaat tgcttaaatt tatcttcacc aaacgcctgc tgttgcgggg aatcgatcat
      721 ttctgcaatt gtttgctggt gcatattccc cagccgatat tgtggataaa catagtgatc
      781 gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag cgcccacagg ttggctgatg
      841 atggcaaacc gcacccggcg caccgacaaa attggcaaac gcccattcga tattcatcac
      901 gaaaatcttg ccgacgtcgc gtttaatcca gtggtcgaat atcgccacca gaaattcacc
      961 gaactcctcg gggcgcaccg accattccgt tagctcaccc tgaatatcac caggcgcatg
     1021 caatttcagt ccttcgcggg cagccgtttc atcagccagg cgctcgacca ccggaataaa
     1081 ctggatgaat tccactcccg catcgcacaa aaaatcatat acctgcaacg gttgctgcgc
     1141 gctggtgcga ttaacgcaga ccagcacgtt atagtcgaca tgatgttttt gcagcagcgt
     1201 cagggcacgc atcaccagct tatgcgtggg tctgccacct ttagtcacgc gatattgatt
     1261 gtggatctca ggcggaccat ccagcgataa accaacaaga aaatgatgct ccgcgagaaa
     1321 cgcgcaccat tcgtcatcca gcagcacgcc gttagtctgg aagctgttac ttatcttcct
     1381 gccagcacca tatttcgcct gtagcgctac ggcacggcgg taaaactcca gcccgagtag
     1441 cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt tgcagttcgc tggcggcgat
     1501 atagtgacgg atatacgctt ccagcgtgtc atcgtccata tgcgtgactg gcttttcgcg
     1561 gtaaagggat tgtttttcga gataaaaaca gtagtcacag ttcagattac aatcggaacc
     1621 actcggtttc gccatcacat gaaaagcacg cgttggaacc tgttgcagca tggtcactcc
     1681 ttgcctgaag tcatccttca atacgttaag ggcgaaatca taccgtgggg caatgagtta
     1741 ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac agaaaacaaa aaaccccgcc
     1801 gaagcggggt tcaaaattgg tcggcgagag aggattcgaa cctccgaccc actggtccca
     1861 aaccagttgc gctaccaagc tgcgctactc gccgaaatac tgctttttga atttttagtt
     1921 caattcttta aagtcgtggt gcgagggggg ggacttgaac ccccacgtcc gtaaggacac
     1981 taacacctga agctagcgcg tctaccaatt ccgccacctt cgcattgtca caacttctaa
     2041 taatggggtg gctaatggga ttcgaaccca cgacaactgg aatcacaatc cagggctcta
     2101 ccaactgagc tatagccacc actacaaatc ttgttacgcg gtattactac caccgcagct
     2161 caagcgccgg gactaaatgg cgcgcccgac aggattcgaa cctgagacct ctgcctccgg
     2221 agggcagcgc tctatccagc tgagctacgg gcgcttagcg ccgttgcggg ggtggataat
     2281 acggacttcc cacccctctg tctagtccct ttttaaataa attgcgcgtt tggttacggt
     2341 ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt cccgtggcga ttaaggccaa
     2401 aaactttata aatcgccgtc accgccagca taaagatgat gccaacaaac agcgacatac
     2461 gcgtatcttc attaaagtac atgccgatca aaacgcaaat caggaatgcc attgttacgt
     2521 agttggccca cgggaacagg attgagcgga acggatggct ggcaatcgcc gctttatgtg
     2581 cacgccgaaa acgcagctgg cttatcaata tcacaaacca tggcaccatc cccggaagca
     2641 cgctggcact gtagacgtag acaaacacac gctgcggatt gggaatgatg tagttcaggc
     2701 atgagccaat tagcagaata gcaatagata ctgccacacc cgcaactggt acgccgtgac
     2761 gggaaacttt cgccatcgcc gccggtaact gacggttttt cgccagtgcg tagagcatac
     2821 gtccgcaact gtacatgccg ctgttacagc cagagagcgc agccgtcagc accacaaagt
     2881 tgataatgcc cgccgctgcg gtaataccga ttttggcaaa agtcagtacg aacgggctgc
     2941 cgttgctgcc aatttcattc cacgggaaga tggtgacgat aacgaaaatc gcgcctacgt
     3001 agaaaatcag gatccgccac agcactttgc ctacggcact gcgcagtgtc acctgcggat
     3061 tcttcgcttc accggcagta atgccaatca gctccacgcc ctggtaggac gccaccacaa
     3121 tacacagcgc ggtcaggaac cctttccagc cacccgcaaa gaaaccgcca tgctctgtga
     3181 gattgctaaa accaatcgac tgcccgccat tgccaaagcc aaagaaaatc acgcccaggc
     3241 caatgacaat catcacgata atcgtggtga ctttgatcat cgcgaaccag aactcgattt
     3301 cgccgtacaa ccgcaccgcc gccagattcg ccaacgccac cagcgccact gcgatcaatg
     3361 cgggtatcca ctgcgccatc tccgggaacc agaactggac ataaacgcca atggcggtga
     3421 tttctgagat ccccaccgcc atccacataa accagtaaga ccaggcggtg agataaccaa
     3481 aaaacgggct catataacga tgcgcataaa cggcgaacga accggtaacc ggttcgagga
     3541 acaacatttc gcccattgaa cgcatgatga aaaagacgaa cagcccggcg atgatatagg
     3601 ccaacaatac ggatggcccg gcccatttca gggtactggc ggcccccata aacaggccga
     3661 cgccaatggt gccccccagg gcgatgagtt cgatatgtcg agcttccagc ccacgctgta
     3721 gctctggttt gttatctgcc ataaatcctc gtgttgtgtt tgcatgcttt ccggtgttac
     3781 cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt cgtaattcag gagaaatggc
     3841 aaataaagca ttaaaaattt gaatgctttg tgtaataaaa aagcagacag gcgacgaagt
     3901 gaccactccg tcgctttaca aagagaggaa aatcagaggt tgccggtgta atgccagcgt
     3961 aaataacgca gcaaacgaag ctgacgctta atgcggctcg gctgcgaaag caggcggtag
     4021 agccactcca gtcccagcgt ttgccagatt ttcggtgcgc gttttacgtg accggtgaaa
     4081 acatcgtaag tcccgccaac gcccatatac agcgcatctg gatgcaccag acggcagtcg
     4141 cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg tgacgatttg cgctccgctg
     4201 gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt taaaataacc atcctgactg
     4261 ccaacgatat tcacattcca ttggttgcgc agtttagctt cagtttgcgc cagcacttca
     4321 ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc ctgcgcgcgc catcagctct
     4381 tcccagagat cggcaccggc aacgcgggaa acctgcgcct gcgggtactt tttacgtact
     4441 gaacgtacaa cgctgatgcc atccgcatat ttaaattcgg cagcgttaat tagctccctg
     4501 acctcagcat tatcttcaat ggtcagcatt ttttcggcat taatggcaac cagcgttccc
     4561 tgcttaagat gcccgtcagc aaacagataa tcgagggcgt gctgcatatc acgccaacca
     4621 atcaactgta agccacgcag cgtataggtc ggtgccgtgg tgttgttatt cattgttatc
     4681 cttcaacctg cgtccggagc gatgattttg tacgtttatg aatgagtccg gcgctttcaa
     4741 aaagccagta caacagtttt gcgaacatca gacatgcgcc gaagaccacg ataaaaaaga
     4801 ctacgcgtga gacaaacgaa tccagccctt cacgtgccag cacgatcata ttgaagatcg
     4861 ccccaaagca gaaactgtgc aatatcgcag ccttatagcg attagtctcg cggttgccca
     4921 gctcatacag ccagtcgaac catttgatga tcagtccaac cacaatcgcc ccgagcggga
     4981 tgaacaacgc gccgcccatc accaccagtg agcctataag cgtaggcgag atcgccagtc
     5041 cggagtggtt attcagcact tcccaggtaa agtagttggc ggagttcagc accatactcg
     5101 ggcgacccgg ccacagccag gaagggataa agacatagaa atcgcggaca attggagcca
     5161 ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa cgccagattc tcccacggcg
     5221 agaaggtgtc gcgagtgaga tagagaaacg tatagaacgc ttcatcgccg ctcacattca
     5281 ttccatagcg ttttagcgcc agccagaaca tgccaacaat ccccagcacg cccgccgccg
     5341 ccagcatcca caacgaaatc cagccgcgaa taatgccaat aaacaggaag atagcgaatg
     5401 cgatgatgat attggcgcga gtgccaccga caatcatata agtcagcaag ccaaaggcga
     5461 ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg gcgcagaaag tagaccacca
     5521 gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc cacgccggag acttcactgg
     5581 aaaagatctg actgtaggag ttaagccgga acagcaaaaa gccgttgtgc atgaagaaga
     5641 tgccgacgct taccagcgcg atacccatca ggatcaccca cgtaagattg gtctccacgc
     5701 gattcatggt aaacagcgga cggcgcggta catcagcaac gcgtttgcgt aggcgggttt
     5761 tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga aagcaacgcc tgcaacaaga
     5821 tttctggcgg cgcgacacca acatcaaagc gaaataccag cacgctggtc agcgggaagc
     5881 cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac attgaagtta aagcgcacac
     5941 ggtgaaactc aaaccaggtc agcgtggcaa taaacagcgt gcagagcagc cagaccacaa
     6001 acaggccact gaattgcagc agactcatgc gacctccccg gcggcaatcg ccaacgcccg
     6061 ctgccagcct tgtagatagt tagggctaaa gaaggcaatg gtgtttttat ccaccgacgc
     6121 caactggcgc tgcgcttcac gcacaatatc ctcgttgaga tcgtcggtag taaacagcac
     6181 cggcaaatgc tgttccgtca tatcctgcca gaacggattt tcccggttaa gcacacaagg
     6241 aatgcccgcc tgaatcagta agcacagcgt accaatgccc tgctggcggg caaaaataaa
     6301 gtaaccgaga tcgcactgac gaagtagcgc cagataggcg tcaaattcca gtttttcgct
     6361 cagaacttgc agattttctt cgctgaataa ctccagcccc gcctgacgaa cttcctcaat
     6421 gtacgcttcg ttattaggcg gatatcccat cggcaccacc acttttaccg tatcgccaaa
     6481 ttgctgatga acggcacgta aggcggcgac atgctcattg ctgcggtcgc cggagttccc
     6541 caccagaatg gtcattttcc cttcacgttg ccgatcgttc gccatcgtat tgagagaagg
     6601 gtccatccgc gtcgggaagt acagcagttc gccccgcacc tttggatgcg ttttggcaaa
     6661 aaagctcaaa tcgccgcggg tggcaaatac acagccgact cgcttttgcg ccaggcgacg
     6721 tagtgggtaa aaaagcttat atctcaagcc actggaaagc tcatacaggt ctgcccccca
     6781 gatatgccag aaaaactggc tgggcttaat gccaccactc agcagagcca gccacagtgt
     6841 gggattgaac tgaccgtgga agaaaaaacg ctgctgacgg ttagcttttg ctttcgcgat
     6901 gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc acagaaagcg ccggacagct
     6961 atcacttaag ccgtcgtcct tgccaacaac cataaactcg cgcgcgtgct cgctcgtcgc
     7021 ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta tggtgaggga tatccgatcc
     7081 cagtacgtga atcagtacag tcatgcccgc ctacgccaga gtaaaaacac gccacaacaa
     7141 agagaaaaat agacgatata agttgccata tatgcctgcg ccgcgcccag tgcaccatgc
     7201 gcagggatta gccagtgggc aaataccatc aataaagtga actggctgac ttccgccaga
     7261 atataaaacc gcagtgacgc tttggcgatc accagataac caaagacata agcgcccact
     7321 tttaacacat cacccactaa ctgccaggca aagagatcgc gcatagcggt aaatttattc
     7381 gacaacagca gccagatagc aaaatcacgc agcagccaga cggtaaaact cgccgccgcc
     7441 actgccggta agacgaattt cagcgattta accacttccc gggtgatatc gcgcttttcc
     7501 gttagccgcg acaacgtggg cagcaaatat acgctgaacg atgccgtaat aaattgcagg
     7561 taggcatcgg aaatactgct caccccttgc cagatcccca cctcatccca gctatactgc
     7621 gccgccagca gtttacgcat catgatgtaa gcaacaggca aggtcaccga cgtaatcaac
     7681 gccatgagcg taaatttgct caactgccct gccagaccgt tatcccagct gggtttcaga
     7741 tagcttaacg ggatgacacc acgtttgatc aacatgatgg cggcaggaat taccaccagc
     7801 gcgggaatca gcgccagacc cagcaacgcc ccttcataac cgcccaaacg gtaactgacg
     7861 tagtacgcga gaacgccaat caagctgccg acaatcagcg ataacgcatt acctgcggca
     7921 tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc aggcgatccc catttgcacc
     7981 agcgccacta aacgcaccag cccctgatag tcggtattac caaacaatcc ctggctgatt
     8041 ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag agaaaccaag taccatcgct
     8101 gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat catggtactg ggcaacgtat
     8161 ttggttacac cgttaaagat gccagctccg gcaagcacgc cgagcacggt aatcaactgg
     8221 cggaaatttg ccgccagccc aagccccgcc ggaccaaatg acacagccag caacttaccg
     8281 accagtaacc cggcaccaat cttgaccagt gtactggccg ccgtccacaa ggacgctttt
     8341 gccaacgaca tatcaggaga agtagttcaa caaagtcgca attaccgtac gctgattgac
     8401 gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc tcgctctctt tggtggtgta
     8461 gcgatcttca ccgtggaact caccaaagtg ttcccccgca gggcaaccgt gcagcggaat
     8521 gtaatgaaac accgccatga tttccgcttc tttcagaaag ttaatcaacg cgctccggtc
     8581 atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc tgcacgcagc catcgggaat
     8641 cgacggcagc tcgatacgcc cggctttcgc cagaggcgct aacgcatcgt agtagttttg
     8701 ccacagcgcc agacgttgct ggttgatacg atccgctgct tccagttgcg cccacaggta
     8761 cgcagcttgc agatcggaca tcaaatagct ggagccaata tcgcgccagg tatatttatc
     8821 gacctgacca cggaagaact ggctgcggtt agtgcccttt tcacggatga tctcggctcg
     8881 ttcgattaac gctttatcgt taatcagcgt cgcgccgcct tcaccacccg ccgtgtagtt
     8941 tttggtttca tggaagctaa agcagccaat atgaccaatg gttcccagtg cacgcccttt
     9001 gtaagtggac atcacgccct gagcggcatc ttccaccaca aaaagattat gctttttcgc
     9061 caacgccata atggtgtcca tttcgcaggc cacacccgcg taatggaccg gcacgataac
     9121 gcgcgttttg tcggtgatcg ccgcttcaat cagcgtttcg tcgatgttca tggtgtccgg
     9181 gcgaacatcc acaaaaacga tttttgcgcc acgcagcaca aaggcattgg cggtggagac
     9241 aaaggtgtag ctcggcatga tcacttcatc gccaggctgg atatcgagca gcagcgccgc
     9301 catctccagc gaagcggtgc aggacggcgt cagtaacact ttggcgctgc caaaacgttg
     9361 ctccagccac tgctggcagc gacgggtaaa accgccatcg ccacacagtt tgccgctacc
     9421 cattgccgac tgcatatagt cgagttcggt tcccaccacc ggcggtgcgt taaatggaat
     9481 catgtgatca cctgtataac cagtacgcgg tgctttctac attcgcacca ctttgtatgt
     9541 atcgtttaag cgcggcggtg ttgcccatct gggtcgccac ccgcaaagtt gttttaccgc
     9601 gagcatacgc ccagtttagc gccgtttgca tcagctcagc acctgcaccg cgtccagcca
     9661 gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa agagacatag ccgcgaatat
     9721 cgccggacgc cgcacgtaaa atcagacatt gatgatcaaa ggtgccgcgc acggcatttt
     9781 caatccactg tgcataaaag cgactgctgg cgtcaggcgc ataccacggc gcacgaaaac
     9841 ggctttgcgc aaatgcggcg ctggctaact gacgtaatgc gggaatatcg gtctcttgtg
     9901 ccactacagc accgctatca ctggcattgt tcacgggtag cgccaaatca acttcacctt
     9961 ctaccaggga gaatcccagc tgttgcaggg catccagttc acccgtattt gatgccgcaa
    10021 ttttggcctg cacccgtgac cacggcgcta acgcgtctgg cgtcaggagc ggtgcttcag
    10081 acgtaatgcg cacgatggcg ctgttaacac caaagaaggc gttttcccag gttagtggct
    10141 caatactggc gcggacgggc acgaagtaac tccagcagat attggccgta gccagttttc
    10201 gctaatgaac tggcagcacg cttcacaccc tcgtcatcga gccagccgtt acgccaggcg
    10261 atctcttcca ggcaggcaat cttaaagccc tggcgttttt ctactgtctg cacaaaagtg
    10321 ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc aggcaaatcc gcgcccgagc
    10381 agttcaacgg tcaggttgcc cgcctcgagg tacatctggt tgatggaggt aatctccagt
    10441 tcaccgcgct ccgacggctt cacctgcttt gcgtactcca cgactttact gtcgtagaaa
    10501 taaagcccgg tcaccgccca gtttgacttc ggctgttttg gcttttcttc cagcgagata
    10561 gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt ccgggtccat cacctgatag
    10621 ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac gcagcttcgg actgaagccc
    10681 tgaccgaaga agatgttatc gcccagcacc agacaagaag gttcaccatt aaggaaggtt
    10741 tcaccgatga taaaggcctg cgccagaccg tccgggctgg gctgttcggc atattccagc
    10801 tggataccga actcactacc atcgcccagc aggcgctgga aataaccttt atcttccggc
    10861 gtagtgatga tgagaatttc gcggataccg gccagcatca gcaccgacag cggatagtaa
    10921 atcattggct tatcgtaaat cggcaacagt tgcttcgata cgccgcgcgt aatcggatgc
    10981 aatcgggtgc cggaaccgcc cgccaggata atacctttca tgtttgcctc cgctgaaaat
    11041 tagcctttca gacctaaacg ctcgccctga tagctgccgt cctgcacctg cttccaccag
    11101 ctttcattag ccagatacca ctgcaccgtt ttacgcattc cactttcaaa ggtttcctgt
    11161 ggcagccaac caagttcacg ggcaattttc gaagcatcaa tggcataacg cagatcatgc
    11221 cccggacggt cagcgacaaa ggtgatcaag tcacgatagt gcaccacgcc gtgcggcttg
    11281 ttcggagcca gttcttccag cagctcgcaa atggtttcca caacatcgag attcttacgc
    11341 tcgttgtgac caccaatatt ataggtttca ccgactttcc cggtggtcgc cacgcaatac
    11401 agcgcgcggg cgtgatcttc cacatacagc cagtcacgga tttgctgccc gttgccatat
    11461 accggcagcg gtttacccgc cagcgcgttg aggatcatca gcgggatcag tttttccgga
    11521 aagtggtaag gaccgtagtt attcgagcag ttggtgatca gcgtcggcag accgtaggtc
    11581 cgcagccagg cgcgcaccag atggtcgctg ctggcttttg acgcggaata agggctgctc
    11641 ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg agtgcaggtc accatatact
    11701 tcgtcggtgg agatatgatg aaaacggaac gctgattttt tatcttccgt cagcgtattc
    11761 cagtaagccc gcgccgcttc aagcaatgta taagtcccga caatgttggt ttcaataaac
    11821 gctgccgggc cgtcaataga acggtcaaca tggctttcgg ctgccagatg catgacacag
    11881 tctggctgat gctcagtgaa tacgcgtgcc agttctgccc gatcgcagat atcaactttc
    11941 tcaaaggcaa agtgctcgct ttgcgcgacc ggtgccagcg acatcaggtt tccggcgtag
    12001 gtcagcttat cgaccactac caccgcatcg ctcgtttcgt tgatgatata acgcaccagc
    12061 gccgagccaa taaacccggc accacctgtt atcagaattt ttctcatcag cgccagactc
    12121 ctttggcatc gacgacatac tgctgatgga cattgtcgcc attgataact ttgaactgac
    12181 tatgatcaac cagcatcacc agcacgtctg ccgttgccag cgcctcgtca agctgcgcca
    12241 gagtacaaag cccggtcagt tttttcggca actggtggat gttaggctca acaaccagcg
    12301 tttcgccgct gtgccactgg gcgatcagtt cagcgatttc catcgccggg ctttcgcgca
    12361 ggtcatcaat attcggttta aacgccagac caaagcaggc gattttcagt tcactggcgc
    12421 gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt cacctgatcg ataacccaga
    12481 acggtttgtg atcgttaact tcgcgcgcgg tacggataag ccgcgcctgc tgggggttct
    12541 gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc gcccacgcca gggccaggct
    12601 gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat cagttcccag acgttaatcc
    12661 cctgatcggc acaaatcagc gacaattcat tagcaaaagc gatattcaca tcgcggaagc
    12721 tgttttcggt gagcttacac atttccgccg tccgcgagtt agtgacgaca cactcacctt
    12781 cgaggaaaat tttgtacagt tcgctggcgc gggccgaaca aaccggcgtc ataccaccaa
    12841 tcacgcgatc gtttttaatc agctcgacca ttacctgtcc tggtaacacg cgttccgggc
    12901 agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg cgggaaagtg agatccggac
    12961 gcatctctgc taaccattct gccatcttct cggttgaccc caccggcgag gtggattcaa
    13021 ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga gcgagcagcc gattcaacgt
    13081 aggtcatatc tggctcatga tcgcccttaa acggcgtggg tacagcaatc agccaggcat
    13141 ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc ttctacggca gtttttacta
    13201 cactcgccaa atcaggttcg acgatatgga tttcgccacg attgatggta tcaaccgcat
    13261 gttggttgat atcgacacca attacctgtt tttgccgtga ggcaaacgct gctgccgttg
    13321 gcagcccgat ataacccagt ccgataacag aaatggtcgc aaaactcata gtgatatccg
    13381 attatttttt aacgcttcca gaatgcgaga gcatgcctga ccatcaccat acgggttatg
    13441 ggcgcggctc atagcttgat attcgttttc gtcttttaaa agacgcgtca cttcctcgac
    13501 aattcgctgc ttatccgtgc ctaccagacg caccgtaccc gccgtcaccg cttccggacg
    13561 ctcagtggta tcgcgcatca ccagcacagg tttccccagc gaaggcgctt cttcctgaat
    13621 gccgcctgag tcggtcaaaa tcagccaggc gtggttcatc agccagacaa acggtaaata
    13681 ctcctgggga tcgatcagaa tgacattttt cacatgcccc agaatgcgat tgaccggctc
    13741 tctgacgttc gggttgagat gcaccggata gacaatctgg atgtcctggt gcgtggtggc
    13801 gatgtctgcc agcgcgtggc agatttcttc aaagccacga ccgaaactct cacgcctgtg
    13861 accggtcacc agaatcatct ttttatcggg gtcgataaac gggtaatttg ccgccagttc
    13921 tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc cataacagtg catcaatgac
    13981 tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt tcacgcagca agttttgccg
    14041 ggaagtttcg gttggagaga agtgatacat cgccagatgc ccggtcaatg tacggttagc
    14101 ctcttccggc cacggcgaat agagatcgcc cgtgcgcaga ccagcctcaa cgtgaccaac
    14161 aggaatacgc tgataaaacg ccgccaggct ggttgccagc gtcgtcgtcg tatcgccgtg
    14221 aaccagcacg acgtctggtt tgaactcggc aagaataggt tttagccctt ccagaatccg
    14281 acaggttatc tctgtcaggc cctgtcctgg ctgcattatg ttgagatcgt agtcaggtac
    14341 aatggaaaag agtttcagca cctgatcgag catctcccga tgctgcgcag tgacgcaaac
    14401 tttagcctca aaaaaaggat cttttgccaa cgcatgcacc aacggcgcca tcttgatggc
    14461 ttccgggcgc gtaccaaata cagtcagtac tttcacatcg attctcttcg aataagcggc
    14521 gagcgccttt gcgctcaccg cagcagtgtt gctatttcga gcaacggcgg gttaatgcga
    14581 caccagcccc gatcagcccc ccgacaatgc cccacataat catcaggaag gcacgacgtg
    14641 ggctatcgcg ttttaccggt tcttccggcg tacgcaaata gcgataggtc tgaaaacgcg
    14701 gatccagggt tggaccaaca ttcagggtgt ttaacatggc ccgattctga tcatagtcga
    14761 gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg agcctgaagc attggacgcc
    14821 caagcaggaa catttctgaa tcaggtaatt cctcggcagg tacatctgtc gcactgcgcg
    14881 aaatattatg ctgctcagca attttcagcg cctgctcaat gctgttcatc cggcggtcgt
    14941 agatggcttt cgccacctct tcctgacgct tcacctgagc tttcatctgg atggtacgcg
    15001 ccgcccatgc gcctttcagc tcatcattca gatggctggc tgcacgctgg ctggcaaaag
    15061 caacatactg acgtaacagg ttattagcgt caggcgcggt ttcggcaata agcttcacgc
    15121 tgtcattgac cgcgcgggta aagtctccgg ggataaactg gatgttgtta atcatttcat
    15181 ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg ctgtgtgtaa tagtcggttt
    15241 gcagccagaa ctctctgcgg gtatcccacg aggccagttg cataacaaac tctttatagg
    15301 cttcgtccat gaccgatggt tggtcggcag cagccatgtt tgaacggaca tccaggttac
    15361 gcaaaaattg ctgctgcgag taatatcccc ccagcatatt caccgttgga cgatcggtaa
    15421 tcgccgtcga gctccactcc tgacgagcaa aaaacgtata cgccagcgcg attaacgcaa
    15481 acgccagccc catgccaata atccatagct tcccagccca caaggtacga aacaacccac
    15541 gaatatccag ttcattttca gcgtcttcgg ccggtttccc aggcattggt tgtgtcatca
    15601 catcctcatt tatttggtta aattggggct gccaccacga tttctacgca gtctgcgttt
    15661 tacgcgctta ataaagcgag caactttcca ggcacgctta atgcaatatc catagaggaa
    15721 gaatgctagc aaaaagagca ccagcatgac ccactccggg acaaaatgag aatattctgc
    15781 cagcacgcca atggaagcga gcagtgctgc ggcaagggta atcagcacaa acgcctgacg
    15841 ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc tgacggtcag gagagaatgg
    15901 gctcatgcct ttacgcaggc gacggtacat aatcgccacc atatccatta gcggaatggc
    15961 gattatccac aaagcggtaa ccgggctgat gggatgggtt ttgccctggg tcgtttcgag
    16021 caggatccag ataacggtaa aaccaatcag cgtactgccc gcatcaccca taaagacttt
    16081 gtagcggcga cccaggatac caaggttaag catgatgtat ggcaggatgg cggcgatcat
    16141 cgcaaagcac cagattgcga ggctggtttg cccgtcgaac cacaaaatca taccgattgc
    16201 tgcaaacgag acgcaggaca acccgcccag caagccatca atgccatcaa ccatgttgaa
    16261 cgcattaatg gccgcccaga cggcaaatag cgtcaggaag taaccaaacg gtccgagcac
    16321 catctcccag gagccaaaga tataacccag gctactgaga taaagcttgc cgaacaccat
    16381 cataacaatg ccaacagcgg cctgtatggt ggcacggatt tttacgctga tatcaaaacg
    16441 gtcatccagc gcgccaatga aaacaagcac accggcacaa gcgagataga gagatgcatg
    16501 cggaatatag taatcgacaa ttccgaacgt gaagcaaatc cctgcgtaaa ccgaaatccc
    16561 cccaacgaga ggtatcaatc cctggtgacg tttgcggaag tttggtttat ccactaaacc
    16621 gacttttttt gccaccttac gggcaaaaaa cagaaacagt gtcgtgaata aaaaaatact
    16681 gatgagatca gtactcactg tcagtaaatt cacaatgcat gctctcagag aaaattatta
    16741 gcagaagtat aaccacgaag acctttattc agaagggaaa gtccgttccg aacccaccag
    16801 tcctgtgcaa aaattaacca tttgatatgc aaattgcata acacgccagc aaattccagg
    16861 aattttccta acatcgccag cgcggcatta agattacaga tataaaagca aaacgccacg
    16921 taaacacgtg gcgtttttgg cataagacaa atttatgagc gtttcatcat ttcgaagaaa
    16981 tcgtcattgg tcttggtcat ggccagttta ttaatgagga attccattgc atcgatttcg
    17041 cccatcgggt gaatgatttt gcgcaggatc cacattttct gcagttcttc ctgagtcgtg
    17101 agcagctctt ctttacgggt accggaacgg ttgtagtcga tagccgggaa gacgcgtttt
    17161 tcagcgatct tacgagagag gtgcagttcc atgttgcctg tacctttaaa ctcttcgtaa
    17221 ataacttcgt ccattttaga accggtatcg ataagcgccg tcgcgataat ggtcaggctg
    17281 ccgccctctt ccacgttacg cgccgcaccg aagaagcgtt tcggacgatg aagggcattg
    17341 gcgtccacac caccggtcaa cactttacct gacgccggaa caacagtgtt gtaagcgcgc
    17401 gccagacgag tgatggagtc gagcagaatg ataacgtctt tcttgtgctc aaccaggcgt
    17461 ttggccttct cgatcaccat ttccgcaacc tgaacgtggc gagatgcggg ttcgtcaaag
    17521 gtagaagcaa caacttcacc ttttaccaga cgctgcatct cggttacttc ttccggacgt
    17581 tcgtcgatca gcagaaccat cagcacacaa tccgggtggt tgtaagcaat gctctgagca
    17641 atgttctgca gcagcatagt tttaccggct ttcggcggtg ccacaatcag accacgctga
    17701 ccacgaccga taggtgatgc cagatccagt acgcgagcgg ttaaatcttc cgtagaaccg
    17761 ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg gggttaagtt ctcaaagagg
    17821 attttgttgc gggcgttttc aggtttgtcg aagttaactt cgttaacttt cagcagcgca
    17881 aaatagcgtt caccttcttt cggcgggcga atcttaccag agatggtatc accagtgcgg
    17941 aggttgaaac ggcggatttg gctaggggaa acgtagatgt catcaggacc ggcgaggtag
    18001 gagctgtctg cggaacggag gaaaccaaat ccatcctgca atatctccag tacgccatca
    18061 ccaaagatat cttcgccact ctttgcgtgc tgcttcagga tggcaaaaat aatgtcctgc
    18121 ttacgcatac gagccaggtt ttccagcccc atattttcgc cgagagtgat cagctcagaa
    18181 accggcgtat tctttaattc ggtaagattc atagtggtgt gagttcttaa acttggggta
    18241 aatctcgaac ttaatgttgt gaatggtatg gcaggatcat ccatgcctgt ttacggtcat
    18301 cgactcatgt cttttcgctg cctggtcaca ggagagtacg cagaactgaa acgacaagac
    18361 ggattgagtg acgagccaga aatctgttca cttcgcattt aagataaaac gggaagcgtt
    18421 ggaaattaca agattcaaac ttaataaggt atgtttaata cgaagtcaac actaagttag
    18481 catgactcac gccgggcgtc cagtttttag cgacggggca cccgaacatg aaattccctt
    18541 acgccaggtt agcgtcgagg aactctttca actgaccttt agacagtgca cccactttgg
    18601 ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg gataccacgg atgccatatt
    18661 tcggcgcagt gccagggttt tgatcgatgt tcagttttgc aacggtcagt ttgccctgat
    18721 attcgtcagc gatttcatcc agaatcgggg cgatcatttt gcacggaccg caccactctg
    18781 cccagaaatc gacgaggatc gccccgtccg ctttgagtac atccgtgtca aaactgtcgt
    18841 cagtcaggtg aataatttta tcgctcatat ataactccac aggaataagc ctggcgtgtt
    18901 ggtttcgttg ttggtgtaac attaaccaac taaaggttga ctttatttca ccggatacgc
    18961 tttcgtaaag caatagtaag ctgatattct accacactat gagcaaaaca catttaacag
    19021 aacagaagtt ttccgacttc gccctgcatc cgaaggttgt agaagccctt gaaaaaaaag
    19081 ggtttcataa ctgtacgccc attcaggcac tggcccttcc gctgacgctg gcgggtcgtg
    19141 acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat ggcgtttctt acgtcaacgt
    19201 ttcattatct tctctctcat cctgcgattg ccgatcgcaa ggtgaatcaa ccgcgtgcct
    19261 taattatggc accgacgcgt gaacttgccg tgcagatcca tgccgacgca gaaccgctgg
    19321 cggaagctac tggcctgaag ctgggtctgg cttacggtgg tgatggctac gacaaacagc
    19381 tgaaagtgct ggaaagcggc gttgacattc tgattggcac cacgggtcgt ttaattgact
    19441 atgccaagca gaaccatatt aacctcggtg ccattcaggt agtggtactg gacgaagccg
    19501 atcgcatgta cgatctgggc tttattaaag atatccgctg gctgttccgc cgtatgccgc
    19561 ctgcaaacca gcgcctcaac atgctgttct ccgccacgct ttcgtaccgg gtacgtgaac
    19621 tggcgttcga gcagatgaac aatgccgaat atattgaagt ggaaccggaa cagaaaacgg
    19681 gccaccgtat taaagaagag cttttctacc cttctaacga agaaaaaatg cgtttgctgc
    19741 aaacgctgat cgaagaagag tggccagaca gagcgattat tttcgccaat accaaacacc
    19801 gttgtgaaga gatctggggc cacctggcgg cagatggtca tcgtgtcggt ttgttgacag
    19861 gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga atttacccgt ggcgatctgg
    19921 atattctggt tgccaccgac gttgccgcgc gtggtttgca tattccggca gtgacgcacg
    19981 tctttaacta cgatttaccc gatgactgtg aagattacgt tcaccgtatt ggtcgtacag
    20041 gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg tgaagagtat gcattgaatt
    20101 tgcctgctat tgagacctat attggtcact caattccggt aagcaaatac aatccggacg
    20161 cattgatgac cgatctgcca aaaccgctgc gcctcacgcg cccgcgcaca ggcaatggtc
    20221 cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg ttaaataaaa aatgtcatac
    20281 tcaaaatcat tcaaactgta tcaagatggc aagagaatga atcccgatga gcttactgta
    20341 gtaagtgatt caggtgaaag aatgcagcca acacagagac agattgaagg atgaagagta
    20401 tgggttccac ctcgtcgctg tatgcagcca ttgatctcgg ttcgaatagt tttcatatgc
    20461 tggttgtgcg cgaggtggct ggaagcatcc agacgctgac gcgaataaaa cgcaaagtgc
    20521 gtctggctgc tggcctgaat agcgaaaatg ccctctccaa tgaagcaatg gagcgcggtt
    20581 ggcaatgttt gcgcctgttt gctgaacgtc tgcaagatat tcctccctcg caaattcgcg
    20641 ttgtcgctac ggcgacgtta cgccttgccg tcaatgcggg tgattttatt gccaaagcgc
    20701 aggaaattct cggttgtccg gtacaggtga tcagcggtga agaggaagca cgtctgattt
    20761 atcagggcgt tgctcacacc actggtggtg ccgatcagcg cctggtggtg gatataggcg
    20821 gagccagtac tgaactggta accggcacgg gtgcacaaac cacctcgttg ttcagcctgt
    20881 cgatgggctg cgtcacttgg ctggaacgct attttgccga tcgtaatctg gggcaggaaa
    20941 attttgatgc tgcagaaaaa gcggcacgcg aagtgttacg tccggttgcc gatgaattac
    21001 ggtatcacgg ctggaaagtg tgcgttggtg cttccggcac cgtgcaggcg ttacaggaaa
    21061 tcatgatggc acaggggatg gatgaacgca ttaccctgga aaagttgcag caattgaaac
    21121 agcgagccat tcattgcggt cggctggagg aactggagat tgacgggctg acgctggaac
    21181 gtgcgttagt gttcccgagt ggtctggcga tcctgatcgc catttttacc gaactgaata
    21241 ttcagtgtat gaccctggcg ggcggtgcgc tgcgtgaagg cctggtctac ggcatgttgc
    21301 atctggccgt cgagcaggat attcgcagcc gtacgctgcg taatattcaa cgccgcttta
    21361 tgatcgatat tgatcaggca cagcgcgtag ccaaagttgc ggctaacttc ttcgatcagg
    21421 tggaaaacga atggcatctt gaagcaataa gccgcgattt gctcatcagc gcctgtcagc
    21481 ttcatgaaat cggcctgagc gttgacttca aacaagcgcc gcaacacgct gcttatctgg
    21541 tgcgtaatct ggatcttccc ggttttaccc ccgcacagaa aaaattgctg gcgacgctac
    21601 tgctcaacca gactaatccg gtcgatctct catcgctgca tcagcaaaat gccgtaccgc
    21661 cgcgcgtcgc agaacaactc tgccgtttac tacgcctggc catcattttt gccagccgtc
    21721 gccgtgacga tctcgtgcca gagatgacat tacaggctaa ccatgaactg ttgaccttga
    21781 cgcttccgca aggttggcta acccaacatc cgctgggtaa agagattatt gctcaggaaa
    21841 gccagtggca gagctatgtc cactggccgc tggaagtgca ttaatgagta agtgccggat
    21901 gcgatgctga cgcatctttt ccggccttga ttatttccct cgttttgccg ccatcatcgc
    21961 tttcagattc gccagatggc tttgcccttt ctgcatccgt tcttcggcgc tgaccacttt
    22021 gcgctcctgt tcccaaatca gatcatcctg cggcagctcc agcaaaaagc ggctcggctc
    22081 cgggcgcacc agttcgccgt actgacggcg ttctttacac agcgtaaagg tcaattcctt
    22141 ctgggcgcgg gtaatgccga cataggccag ccgccgctcc tcatcgatat tatcttcatc
    22201 gatgctgctc tggtgcggca aaaacccttc ttccataccg accatgtaga cataaggaaa
    22261 ctccagccct ttcgacgcgt ggagagtcat cagttgcacc tgatccagct cttcttcact
    22321 ctcaccacgc tccatcatgt cgcgcaaagt aaagcgcgtc accacctggg tgagcgtcat
    22381 cggctcatcc agttcactgc cttccagcat ctccgtcatc cagctaaaca gttggttgac
    22441 gttcttcatg cgcatttcgg cggctttcgg gctgggcgat gtttcgtaca gccaggattc
    22501 ataatccatg ccatggatca gatcacgcac cgcggcaatc ggctcccgct ccgccagacg
    22561 ctggatttct gccaaccagt gagtgaagcg ggtcaatgct tcataaccac gtccgctaag
    22621 cgtctgactc aggcccatat caaagctggc ggtaaacatg cttttattgc gcgtcatcgc
    22681 ccactcaccc agctttttca gcgtagccgg gccaatctct cgcttcggcg tgttaacgat
    22741 acgcagaaat gcgctgtcat cgtccgggtt agtcagcacg cgcagataag ccagcaagtc
    22801 cttgatttca ggacgagaga aaaacgacgt accaccagat attttgtacg ggatgcggtt
    22861 ttgcatcagg aatttttcaa acacccgcga ctgatggtta ccgcgataaa gaatggcgta
    22921 atctttgtac tgcgttttat tgacgaagtg atgggcgatc agctcgccag taacgcgctc
    22981 agcctcatgt tcttcgttat tcgcgcttaa tacttttagc tccgcgccat aacccagttc
    23041 ggagaacaga cgcttttcaa agacgtgcgg gttattggcg atcaggatgt tcgccgcttt
    23101 cagaatacgc ccggaagagc gatagttctg ctcaagctta atcaccttca gcgccggaaa
    23161 atcctgactc agcagcacca ggttttgcgg acgtgcaccg cgccaggagt agatcgactg
    23221 gtcatcgtca cccaccacgg taaagcgcgc gcggctgccc accagcagtt tcaccagctc
    23281 atactggctg gtgttggtgt cctgatactc atccaccagc agatagcgaa ttttgttctg
    23341 ccagcgctcg cggacttctt cattgcgttg cagcagcaac gtcggcaata aaatcagatc
    23401 atcgaagtcg agaacgttac aggctttcag gtgtgcatca tacagcccat aacaatgggc
    23461 aaaaatacgg tcccgctcgc caatcgcact tgctgccgcc tgggacggtg ttttgagatc
    23521 attcttccag ttagagatgg tcgaaatcag ttgttgcagg agaactttgt catcttcaat
    23581 cagcccctcg gtcaactctt taagcaaagc aagctgatcg gtatcgtcaa acaacgagaa
    23641 gttcgctttc atcccaagcg ccgcatactc gcgtttgatg atatccagcc ccaacgtatg
    23701 gaaagtggag atcatcagcc cacgcgcctc tttgcgcccc agcgtctgcc ctacacgctc
    23761 tttcatctcg cgcgctgctt tattagtaaa ggtcaccgcc gcaatgtgcc gcgcctgata
    23821 accgcaaccg cggatcagat gggcgatttt attggtgata acacgagttt taccggaacc
    23881 cgcgcccgcc agcaccaggc aggggccggt aacgaattcg acagcttgtt gttggccggg
    23941 gtttagacgc ataggtgtat tgctcaatct tcgaacgggg ggaggattgt agcagaatcg
    24001 catttgaatg tgaccgcgtg taagccgcta accggttggc tgcggatgtt taaatcagag
    24061 agagaagacc gaaatcggag ttcatcgtga atccattaga cttagaaaat atcgggtcgt
    24121 aacgttgtgt tattactttt tcagagttta ctaaggagag gtttactcgt ggcgattaat
    24181 ttcagcccta aggttggtga aatactggaa tgcaactttg gtaactatcc tgttagccaa
    24241 aatggaccgt tcagcacgac gtattacgat ggccgcattc ctcctgaaat gataaaaaac
    24301 aggttagttg tcgtgctcaa cggaaaaatt aatggcaacg catgcatcgt cgttccatta
    24361 tcaaccactc gcgaccagac aagttaaaac gtggaatgca tgttgaaata gccagtaatg
    24421 ttatcaatga cctgcaattc tttgatcagc agattcgttg ggccaaagcc gacctggtac
    24481 aacaagtcag tcgaaacaga ttaaacagag caaggactta cagagggtat cttaatcaat
    24541 gtttgccaca tgagttggtt gcagacatac agagggcggt catcaagtca atcaatgcca
    24601 tatcgctcat aaattgacaa taaaataacc atccccgata atgactttgt agcccataga
    24661 ggccctaagt tttctcccct tgagggacct gcgaagcccg gattttccgg gcttttgcca
    24721 tatatgtacc agccactccc tgactgacaa ttacgttagc atctgcggat catgctaaaa
    24781 tcgccgcctg acaattattc actcaaggca cgatcatggc aaaaacagca gcagcactgc
    24841 atatccttgt aaaagaagag aaactggctc tggatcttct cgagcagatt aagaacgggg
    24901 ccgatttcgg caagctggcg aagaaacact ccatttgccc atcaggcaaa cgcggcggtg
    24961 atttaggtga attccgccag ggtcagatgg ttccggcgtt cgataaagtg gttttctctt
    25021 gtccggtact ggagccgacc ggcccgctgc acacccagtt cggatatcac atcattaagg
    25081 tgctgtaccg caactaatag caaggccttc tccaggagaa ggccttgagt gttttctccc
    25141 tctccctgtg ggagagggtc ggggtgaggg catcagcgcg cacttaaccc gcaacagcaa
    25201 tacgtttcat atctgtcata tagccgcgca gtttcttacc tacctgctca atcgcatggc
    25261 tgcgaatcgc ttcgttcaca tcacgcagtt gcccgttatc taccgcgcct tccggaatag
    25321 ctttacccag gtcgcccggt tgcagctctg ccataaacgg tttcagcaac ggcacacaag
    25381 cgtaagagaa cagatagtta ccgtactcag cggtatcaga gataaccacg ttcatttcgt
    25441 acagacgctt acgggcgatg gtgttggcaa tcagcggcag ctcgtgcagt gattcataat
    25501 atgcagactc ttcaatgatg ccggaatcga ccatggtttc gaacgccagt tcaacgcccg
    25561 ctttcaccat cgcgatcatc agtacgcctt tatcgaagta ctcctgctcg ccgattttgc
    25621 cttcatactg cggcgcggtt tcaaacgcgg ttttgccggt ctcttcacgc caggtcagca
    25681 gtttcttatc atcgttggcc cagtccgcca tcataccgga agagaattcg ccggagatga
    25741 tgtcgtccat atgtttctgg aacaggggtg ccatgatctc tttcagctgt tcagaaagcg
    25801 cataagcacg cagtttcgcc gggttagaga gacggtccat catcagggtg atgccgccct
    25861 gtttcagtgc ttcggtgatg gtttcccaac cgaactgaat cagtttttct gcgtatgctg
    25921 gatcggtacc ttcttccacc agcttgtcga agcacagcag agagccagcc tgcaacatac
    25981 cgcacaggat ggtttgctcg cccatcaggt cagatttcac ttccgcaacg aaggacgatt
    26041 ccagcacacc cgcacggtga ccaccggttg cagccgccca ggctttggca atcgccatgc
    26101 cttcgccttt cggatcgttt tccgggtgaa cggcaatcag cgtcggtacg ccgaacccac
    26161 gtttgtactc ttcacgcact tcggtgcctg ggcatttcgg cgcaaccatc actacggtga
    26221 tatctttacg gatctgctcg cccacttcga cgatgttgaa accgtgcgag tagcccagcg
    26281 ccgcgccgtc tttcatcagt ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg
    26341 gcgtcaggtt aatcaccaga tccgcctgtg ggatcagttc ttcgtaagta cccactttaa
    26401 aaccattttc ggtcgcttta cgccaggacg cgcgcttctc ggcaatcgct tctttacgca
    26461 gagcgtagga gatatcgaga ccagaatcac gcatgttcag gccctggttc agaccctgtg
    26521 cgccacagcc gacgatgact acttttttac cctgaaggta gctcgcgcca tcggcgaatt
    26581 catcgcggcc cataaagcga catttgccca gctgtgccag ctgctggcgc agattcagtg
    26641 tattgaagta gttagccatg gtgattcctc gtgatgttgt gcttcttatt gttcggttcg
    26701 cgtttgcgag attgaattca ctatatgaca ggaaatttat tgcggaaatt gatatattca
    26761 caacgtcaca ttgcaatttt tgcaacgtca acatcgaggg ctgtccctgt ggatttacgc
    26821 gatctgaaaa ccttcctgca tctggcggaa agccgccatt ttggccgcag cgcgcgggcg
    26881 atgcacgtta gcccatccac gctctcacgg cagattcagc gcctggaaga agatctcggt
    26941 cagccgctgt ttgtgcgcga taaccgcacg gtgacgctga ctgaagcggg cgaagagctg
    27001 cgcgttttcg cccagcaaac gctgttgcag tatcagcagt tgcgccacac catcgatcag
    27061 caagggccgt cgctctctgg cgaattacat atcttctgct cggtgaccgc tgcctacagc
    27121 catctgccgc cgattctgga tcgcttccgc gcggaacacc cgtcggtgga gattaaactt
    27181 actactggtg atgcggcaga tgcgatggaa aaggtggtca ctggtgaagc ggatctggcg
    27241 attgcgggta aaccggaaac cttgcccggc gcagtggcgt tttcgatgct ggagaatctg
    27301 gcagtagtgc tgattgcccc cgcgctgccc tgcccggtgc gtaatcaggt gtcggtagag
    27361 aagccggact ggtcaacggt gccgtttatt atggccgatc aggggccggt acgccgccgc
    27421 attgaactgt ggtttcgacg caataaaatc agtaacccga tgatttacgc cacggttggc
    27481 gggcatgaag cgatggtatc gatggtggca ctcggctgtg gcgtggcatt gttgccggaa
    27541 gtggtgctgg aaaacagccc cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc
    27601 gatgagaaaa cgccgtttga gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg
    27661 ctaattgagg cattctggaa gattttgccg aaccacaaat gacgttgtcg cgcgggtagg
    27721 cctgataagc gaagcgctat caggcatttt tccctaaccc gccaaaaaga acctgaacgc
    27781 cgggttattg gtttcgtcgt ggcaatcgta gcccagctca ttcagccggg tttcgaaatc
    27841 cggttcatgg tcgccaagtt cgaacgccgc cagtacgcgc ccgtagtcgg tgccatggct
    27901 gcgatagtgg aacaaagaaa tgttccagta cgtacccagc gtgttgagga agcgcagcag
    27961 cgcgcccggt gattccggga attcgaagct gtagaggcgt tcctgcaacg gatgcgatgg
    28021 acgtccgccg accatatagc gcacgtgtag cttcgccatt tcgtcgtcgg agagatcaac
    28081 cacgctgtag ccgccgtcgt tgagcatctg caaaatttct ttgcgctctt cgaggccgcg
    28141 gctcaggcgc acaccgacaa agatgcaggc gtttttggca tcggcaaaac ggtagttgaa
    28201 ctcggtgacc gaacgcccgc caagcagttg gcagaatttg aggaagctgc ctttttcttc
    28261 cggaatggtc accgccaaca acgcttcacg ctgttcgccc agttcgcagc gttctgagac
    28321 gtagcgcagg ccgtggaagt tcacgttggc accggaaaga atatgcgcca gccgttcgcc
    28381 gcgaatgttg tgcagggcga tatatttttt cattcccgcc agcgccagcg cgccagaggg
    28441 ttccgccacc gcgcgcacat cttcgaataa atccttcatc gccgcacaga tcgcatcgct
    28501 atcgacggtg atgatgtcgt cgagatactc ctggcataaa cggaaggttt cgtcaccgat
    28561 gcgttttacc gctacgcctt cagcaaatag ccctacgcgc ggcagatcaa ccggatgacc
    28621 cgcatccagc gctgctttca ggcaggcgga gtcttccgct tctacggcga tcactttgat
    28681 ttgcggcatc agttgtttga tcagcaccgc cacgccagca gccagaccgc cgccgccgac
    28741 tggcacaaat acgcggtcga gatgggcgtc ctgctggagc agttccagcg ccagcgtgcc
    28801 ttgcccggca atcaccatcg gatggtcgaa cggcggcacc caggtgaacc cctgctgctg
    28861 tgacagttcg atcgctttgg ctttcgcttc atcaaagttc gcaccgtgga gcagcacttc
    28921 gccgccgaag ccgcgcaccg catcaacttt gatatcggcg gtggcggttg gcatgacgat
    28981 cagtgccttc acgcctaacc gtgcggaaga aaacgcgacg ccctgcgcgt ggttacccgc
    29041 agaagcggtg atcacgccgt gcgctttttg ttcttccgtc aggcccgcca tcatggcgta
    29101 tgcaccgcgc agcttaaagc tgtgcaccgg ctggcgatct tcgcgcttca ccagaatcac
    29161 gttatcaaga cgcgacgaca gtttttccat tttctgtagc ggcgtaacct gcgccgcttc
    29221 atagaccggc gcgcgtagca ccgctcttaa atattcggca ccttccgggg tgccggacag
    29281 gggttgcgag tcagccatca ttaacccccc agtttcgatt tatcgcgcac cgcacctttg
    29341 tcggcgctgg tcgccaggct ggcgtaggca cgcagcgcaa aggaaacctg acgttcacgg
    29401 tttttcggcg tccaggcttt gtcaccccgg gcttcctgcg cttcacgacg cgccgccagt
    29461 tcggcatcgc ttacctgtaa ctgaatacca cggttcggaa tgtcgatagc gataagatcg
    29521 ccgtcttcaa tcaggccaat gctgccgccg cttgccgctt ccggtgagac gtgcccgata
    29581 gaaaggccag aggtgccgcc agagaaacga ccgtcggtga tcagcgcaca ggctttaccg
    29641 agccccattg atttcaggaa gctggttggg tagagcattt cctgcatccc cggaccgcct
    29701 ttcgggcctt catagcgaat tactaccaca tctccggcga caactttacc gccgagaatc
    29761 gcttctaccg cgtcgtcctg gctttcgtac actttcgccg ggccggtgaa tttgaggatg
    29821 ctgtcatcga cgcccgcggt tttaacgatg cagccgtttt ctgcgaaatt accgtagagc
    29881 accgccaggc cgccgtcttt gctgtaggcg tgttccagcg agcggataca gccgtttgcg
    29941 cgatcgtcat cgagagaatc ccaacggcaa tcctgcgaga atgcctgtgt agtccgaatg
    30001 cccgccgggc ctgcgcggaa catatttttt accgcgtcat cctgggtcag cataacgtcg
    30061 tattgttcca gcgtttgcgg caacgtcagg ccaagtacgt ttttcacatc acggttcagt
    30121 aaccccgcac gatccagttc gccgagaata ccgataacac caccagcacg atgaacatct
    30181 tccatatggt atttctgggt gctcggcgca actttacaca gctgcggaac cttgcgggag
    30241 agcttatcga tatcactcat ggtgaagtcg atttccgctt cctgcgccgc cgccagcagg
    30301 tgaagtacgg tgttagtcga tccacccatc gcgatatcca gcgtcatggc gttttcaaac
    30361 gccgccttac tggcgatatt acgcggcagt gcactttcgt cgttttgctc gtaataacgt
    30421 ttggtcaatt caacaatgcg tttaccagca ttaaggaaca gctgcttacg gtccgcgtgg
    30481 gttgccagca gcgagccgtt gcctggctgc gacaaaccca gcgcttcggt caggcagttc
    30541 attgagttag cggtaaacat cccggagcag gaaccgcagg ttgggcaggc ggaacgttca
    30601 acctgatcgc tctgggagtc agagactttc gggtctgcgc cctggatcat cgcatcaacc
    30661 agatcgagct tgattatccg atcggacagc ttggttttcc cggcttccat cgggccgccg
    30721 gaaacaaaga tcaccggaat atttaatcgc agggaagcca tcaacatccc aggggtgatt
    30781 ttgtcgcagt tggagataca gaccatggca tcggcgcagt gggcgttgac catatactca
    30841 acggaatcag cgatcagttc gcgagatggc agtgaataaa gcatcccccc gtggcccatg
    30901 gcaatcccat catccaccgc aatggtgttg aactctttgg caacgccgcc agccgcttca
    30961 atttgttcgg cgaccagttt accgagatcg cgcagatgga cgtgacccgg tacaaattgg
    31021 gtgaacgagt tcacaaccgc gataatcggc ttaccgaaat cggcgtcggt catcccggtg
    31081 gcgcgccaca gtgcgcgggc ccccgccata ttacggccat gggtggtggt agcggaacga
    31141 tacttaggca tgctttattt actcccagtg tctgtctcgt aaatgggacg gtgcgtgccg
    31201 tcccattttt tgtatttatt gattaacttg atctaaccag ccccatttat cttcggtttc
    31261 gccagtgaag aggccgaaga aggcttgctg gatgcgtttg gtaaccgggc cgcaacggcc
    31321 ttcaccaacc tgaataccat ctacgctgcg cactggcgtg atttctgccg cagtaccgga
    31381 cataaacact tcatccgcca ggtacaggga ttcgcgcgac agcacctgct cacggacttc
    31441 aattcccagc tcttttgcca gtttgatgat ggcatcacgg gtaatacccg gcagcgcgga
    31501 ggaggtgaac ggtggggtga acagcacgcc gtctttcact tcaaacaggt tttcgcctgc
    31561 accttcagag atgtaaccat tcacatccag cgcgatacct tcctgataac cgtggcggcg
    31621 tgcttcacta cccaccagca gggaagagag gtagttacca ccggcttttg ccgcggttgg
    31681 gatggtgttt ggtgctgcgc ggttccagga ggaaaccatc gcatcgatcc cctgctccag
    31741 cgcttctgcg cccagatacg ctccccacgg gaaagcggcg ataatcacat cggttgagta
    31801 tcccgctggc gggttaacgc ccatgccaac atcaccgacg aagatcagcg gacggatata
    31861 ggcgctggtg agattgtttt tgcggatcac gtcacgacaa gcttccatca gctcatcaat
    31921 gctctgcgaa accggaaagc gatagatttt ggcggagtca tgcagacgct gcatatgctc
    31981 acgatggcgg aatacaaccg gtcctttgtg cgagtcgtag caacggatgc cttcaaaaac
    32041 cgaggtgcca tagtgcagcg cgtgcgacat cacatgcacc ttcgcgtctt cccagcgaac
    32101 catctcccca ttgaaccaaa tgtaatcagc tttcttcgtg gtcattttta tattcctttt
    32161 gcgctcaggc gcggatttgt tgtgatgtgg ttgtgctctg gcagatggca acgtgtgcga
    32221 cgtccaccag tttatttaac tgactaaaca gtaagtcgac cgaccgtggg ctggcaacgg
    32281 tcaattcgat atttatattt tgtgcatcgc tggcggcggc catattcatt gagcagacgt
    32341 ggaaaccacg atgacgcacc acgcgtaaaa cacgttctaa ggtttctgga ttgaagcgag
    32401 ccgatacatt gacctgatgt tgcatcatga taatttctcc aacatttctg aattactggc
    32461 acctggcggc accagcggcc agacgttctc aagttcgtcg attgagacat gaagcaggta
    32521 tggcccatca ctgttcagca tggtgttgag tgccgcttca acctggtctt tacgggtgat
    32581 gtgttggcca gggatgccga aggcgctggc taacatgagg aaatcggggt tatcagtaag
    32641 ggtggtttcg ctgtatcgtt cctgaaaaaa cagttgctgc cattgtcgaa ccatccctaa
    32701 ccgttggtta tcgagtaaga cgattttcaa cggtaactgc ttgcgtttta cggtgcccag
    32761 ctcttgcaca ttcatcatga aagagccgtc accggagata cagacaacgg tatcgttcgg
    32821 tcgcgcgact tgtgcgccaa ccgccgccgg taaaccaaaa cccatggtac ctaagccgct
    32881 ggaggtgatg aaattttccg ggcgagtgtg ggcgatgtgc tgcgcagccc acatctggtg
    32941 ctgccccaca tctgtggtca cgacgcaatc cgcaggttta cgatccgaca gttgttttaa
    33001 caacaacggc gcgtagatag cgtcaccggg atggtcgtaa cgccaggcat gttcatcacg
    33061 cagctgcgcg cagtgttgct gccagtcatt gatatttaac ggctgctgta atgctggtaa
    33121 cagagcattt aaatcacctt gtaatgccac atgtgcctga cgcagcttgt tcatttctgc
    33181 cgggtcgata tccatatgga taacactggc gtgtggtgcg aaggtgttca gtttgccggt
    33241 cacccggtca tcaaaacgtg cacccacggc gatcagcaag tcgcactcct gcaccgcgaa
    33301 gttcgccgct ttggtgccat gcattcccag catgcccaga tagtacggat aatctgcttc
    33361 aactgcgccc agccctttca gcgtgcaggt ggcaggcatt tttgtggtag cgagaaattc
    33421 tcgtaaagca ggaactgcct gcgccatacc cacgccacca ccaacgtaca gcatcggttt
    33481 ttgcgctttt gccagcatct ggcgcgcttg ctcgacttcg gcatgtggga aagtcacttc
    33541 gttttcaacg gtggtgaacc acggttccag gtcgccgctg gctaattgga tatcttttgg
    33601 gatatcgacc agaaccggac caggacgacc tgagctggca acgtcgaatg cttcagccat
    33661 gatgcgcggc aactcttcca gcgactgcac caggaagctg tgcttggtac aggctagcga
    33721 caatcccagg acatccactt cctgaaatgc gtccgtgccg ataaacggtg cggacacttg
    33781 accggtgatg gcaacaacgg ggatggaatc taacagtgcg tccgcaagcc cggttatcag
    33841 gttggttgcg cccggaccag acgtggcgat acatacgcca gttttgccag tagcacgggc
    33901 ataaccgata gccgccattg ccgcaccctg ttcatgtcgg cacagcaagt gctccacgcc
    33961 gccgtcatac aatgcatcgt aaaccggcat aattgcgcca cccggataac cgaaaacggt
    34021 attcacaccc tgtgcccgca acgcatgtac cacccactgt gcgccattca tagttagttc
    34081 cccgtcctga atcttgagaa acagaatttt gtgctgttat tcattgtctg ctcctcggtt
    34141 atgtttttaa ggtcaaaaaa aacccccgga cctttcggtg cgggggtctt agttcgttaa
    34201 ggcttgattt ctaagccttt cctcgtccaa gtgcagcccc gcacggtggg ataataatca
    34261 ccaccacgct aatcacgacc aggctaatca ctcgtagaag ggctgtcatt ttgtcttttc
    34321 ttgcatcttg ttcgaaggaa tgcttaaaga gttaccatag gttttgtaaa tagtacaaga
    34381 tattttttgg ccgattttta tgatgcgttt aataatttca atagaaattg ttgtttttta
    34441 aaaagaaaat agtgagtgaa aaaaattcaa tttcggtctg aatgttgttc ccttcctctt
    34501 agttcatcct ttgtccgctg tatttttgcg agcatcattc caccgccaga acaactcaac
    34561 ttcacttgca caaataaaca ggaatcagcc actaaaattc acgaattagc gggctacagc
    34621 agcgatatct tatgcacata atggtgactc aaggagggct tatgtcactg tcaattgttc
    34681 atacccgcgc agccctggga gtaaatgcgc ccccaatcac tgttgaggta catatcagta
    34741 aaggtctacc cggcttaacg atggtgggct taccagaaac aacggtgaaa gaagctcgcg
    34801 atcgcgtgcg cagcgccatt atcaatagcg gatatgaata tccggcgaaa aaaatcacca
    34861 tcaaccttgc tccagccgat ctaccaaaag aagggggacg atatgattta cctatcgcca
    34921 ttgcgttgct ggcggcctca gaacagctta cagccaataa gttagatgaa tatgaattag
    34981 tcggagaact ggcgcttaca ggcgctctgc gtggcgttcc cggcgcaatc tccagtgcaa
    35041 ctgaagctat taagtcgggc agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg
    35101 ggctaattaa cggtgaagga tgcctgatag ccgatcatct gcaagctgtc tgtgcgtttc
    35161 tggaaggtaa gcacgctctc gaacgcccga aaccaactga tgcagtatcc cgggcgctac
    35221 aacatgatct cagtgatgtt gtcggtcagg aacatggaaa gcgaggactg gaaattaccg
    35281 ccgctggcgg gcacaacctt ttactgattg ggcctccggg aacaggtaaa acaatgctcg
    35341 ccagccgtat taatggcctg ttgccagatt taagcaatga agaggcactg gagagtgctg
    35401 cgatattaag tctggtaaat gctgaatcag tacaaaaaca atggcggcag cgcccgttcc
    35461 gctcacctca tcacagtgca tcgttaactg cgatggtagg cggtggcgca attccagggc
    35521 ccggtgaaat ttcgctggcg cataacggcg tgctttttct tgatgagtta cctgaatttg
    35581 aaaggcgtac actggatgcc ttgcgagagc cgattgaatc cgggcagatc catctttcac
    35641 gcacgcgagc aaaaataacc tatccagccc gtttccagct tgtcgcggcg atgaatccca
    35701 gccctaccgg acattatcag ggaaaccata accgctgcac accagagcaa acgctgcgtt
    35761 atctcaaccg actctctggt ccctttctcg accgcttcga tctctcactg gagatcccat
    35821 taccgcctcc cggtattttg agtaaaacgg tagtgccggg agaaagcagc gccaccgtta
    35881 aacaacgcgt aatggccgcc agagagcgcc aatttaagcg gcagaataaa ctgaacgcct
    35941 ggctggatag tccggaaata cgccaattct gcaaacttga gagcgaagat gcgatgtggc
    36001 tggaagaaac gctgatccat ctggggttat cgattcgtgc ctggcagcgg ttattgaaag
    36061 ttgcacgaac cattgctgat attgatcagt ctgacattat cacacgtcaa catttgcagg
    36121 aggcagttag ctatcgagcg attgaccgtt tgctcatcca tctgcagaaa ctactgacat
    36181 aaaaaaaggg catttcgccc tttttattaa tcgtcagaat cggtgtagtc ttcagcacct
    36241 tcaacctgcg gtttaccgcc ggaaagggtg tgaaaacgtt ttggacgctt aatacgcgtc
    36301 atatacttgg accacacgcg ttctgcttct gtcactggct cacgttcgcc acggcatact
    36361 gctacgaaga gtttctcttc ctcggtaacc ggctcgcgtt tgccaagatc caactcattg
    36421 aaggcataac catgacgctc aagcagttgt gcctctttga tggtgaaatc accatgacga
    36481 gagaatccac gtggataatg tttattgtcg aaatatcgat tagtcgtcgt aaagctttcc
    36541 gccatcctgc acgctcctaa ttctttgacc gagctagtta tggcgcggag tattagttac
    36601 gcttgacaga gtgtaaaaca aaacatttaa atcataacga caaataattt tgcggagagc
    36661 actgtggata cggaattgtt aaaaactttc ctggaagtta gccgaacgcg tcactttggt
    36721 cgagcggctg aatcgctcta tctgacccag tcagcagtga gctttcgaat cagacaactg
    36781 gaaaatcaac tgggtgtgaa ccttttcacc cgccacagaa acaatatccg tttaaccgct
    36841 gccggtgaaa aactactgcc ttatgcagaa acgctcatga gcacctggca ggccgcccgc
    36901 aaggaggtgg cgcatacctc acgacataac gagttttcta tcggtgccag cgcctcattg
    36961 tgggaatgta tgcttaatca gtggctggga cgtttgtatc aaaatcagga tgcccataca
    37021 ggcttacagt tcgaagcgcg aattgcccaa cggcagtctc tggtaaaaca gctgcatgaa
    37081 cgccagcttg atcttcttat taccactgaa gcgcccaaaa tggacgaatt tagtagtcag
    37141 ttgctgggat atttcacttt agcgctttat accagtgccc cttcaaaact aaagggagat
    37201 cttaattatc tgcgacttga gtgggggcca gattttcaac agcatgaggc aggtttgatc
    37261 ggtgctgacg aagtgcccat tctgacaacc agttctgctg aactggcaca gcaacagatt
    37321 gcgatgctta atggttgcac ctggctaccc gtcagctggg cgcgtaaaaa aggcggcctg
    37381 cataccgttg tcgatagcac aacactttca cggccgcttt atgccatatg gctgcaaaat
    37441 agcgataaaa atgcgttgat tcgcgatctt ttaaaaatta acgtgctgga tgaagtgtat
    37501 taatatgaat ggctggcaag gatgccggta gaaggattta cttcggagag ggttatttca
    37561 gataaaaaaa atccttagct ttcgctaagg atgatttctg gcaggggcgg agagactcga
    37621 actcccaaca cccggttttg gagaccggtg ctctaccaat tgaactacgc ccctaattag
    37681 ggtggcggaa cggacgggac tcgaacccgc gaccccctgc gtgacaggca ggtattctaa
    37741 ccgactgaac taccgctcca ccgaattctt ttacaaccac
//
LOCUS       DAPHBJ010000021        37603 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.021, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000021 DAPHBJ010000000
VERSION     DAPHBJ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.021"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(471..1247)
                     /gene="sseB"
                     /locus_tag="RHZ76_000994"
     CDS             complement(471..1247)
                     /gene="sseB"
                     /locus_tag="RHZ76_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HDV2811672.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(1389..2672)
                     /gene="pepB"
                     /locus_tag="RHZ76_000995"
     CDS             complement(1389..2672)
                     /gene="pepB"
                     /locus_tag="RHZ76_000995"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HDV2811673.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     AAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     MHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(2730..2930)
                     /gene="iscX"
                     /locus_tag="RHZ76_000996"
     CDS             complement(2730..2930)
                     /gene="iscX"
                     /locus_tag="RHZ76_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HDV2811674.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(2942..3277)
                     /gene="fdx"
                     /locus_tag="RHZ76_000997"
     CDS             complement(2942..3277)
                     /gene="fdx"
                     /locus_tag="RHZ76_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HDV2811675.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(3279..5129)
                     /gene="hscA"
                     /locus_tag="RHZ76_000998"
     CDS             complement(3279..5129)
                     /gene="hscA"
                     /locus_tag="RHZ76_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HDV2811676.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5146..5661)
                     /gene="hscB"
                     /locus_tag="RHZ76_000999"
     CDS             complement(5146..5661)
                     /gene="hscB"
                     /locus_tag="RHZ76_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HDV2811677.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(5757..6080)
                     /gene="iscA"
                     /locus_tag="RHZ76_001000"
     CDS             complement(5757..6080)
                     /gene="iscA"
                     /locus_tag="RHZ76_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HDV2811678.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6097..6483)
                     /gene="iscU"
                     /locus_tag="RHZ76_001001"
     CDS             complement(6097..6483)
                     /gene="iscU"
                     /locus_tag="RHZ76_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HDV2811679.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(6511..7725)
                     /gene="iscS"
                     /locus_tag="RHZ76_001002"
     CDS             complement(6511..7725)
                     /gene="iscS"
                     /locus_tag="RHZ76_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDV2811680.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(7966..8454)
                     /gene="iscR"
                     /locus_tag="RHZ76_001003"
     CDS             complement(7966..8454)
                     /gene="iscR"
                     /locus_tag="RHZ76_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HDV2811681.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(8724..9464)
                     /gene="trmJ"
                     /locus_tag="RHZ76_001004"
     CDS             complement(8724..9464)
                     /gene="trmJ"
                     /locus_tag="RHZ76_001004"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HDV2811682.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKTE"
     gene            9583..10386
                     /gene="suhB"
                     /locus_tag="RHZ76_001005"
     CDS             9583..10386
                     /gene="suhB"
                     /locus_tag="RHZ76_001005"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HDV2811683.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            10531..11385
                     /locus_tag="RHZ76_001006"
     CDS             10531..11385
                     /locus_tag="RHZ76_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV2811684.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSSGPADNTIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDLNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            11576..12856
                     /gene="csiE"
                     /locus_tag="RHZ76_001007"
     CDS             11576..12856
                     /gene="csiE"
                     /locus_tag="RHZ76_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HDV2811685.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGMTPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(12848..13987)
                     /locus_tag="RHZ76_001008"
     CDS             complement(12848..13987)
                     /locus_tag="RHZ76_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HDV2811686.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLIFALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14147..15037)
                     /gene="hcaR"
                     /locus_tag="RHZ76_001009"
     CDS             complement(14147..15037)
                     /gene="hcaR"
                     /locus_tag="RHZ76_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HDV2811687.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            15173..16534
                     /locus_tag="RHZ76_001010"
     CDS             15173..16534
                     /locus_tag="RHZ76_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HDV2811688.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            16531..17049
                     /locus_tag="RHZ76_001011"
     CDS             16531..17049
                     /locus_tag="RHZ76_001011"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HDV2811689.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17049..17369
                     /locus_tag="RHZ76_001012"
     CDS             17049..17369
                     /locus_tag="RHZ76_001012"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HDV2811690.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHIEGGDIFIDLP
                     EAQP"
     gene            17366..18178
                     /gene="hcaB"
                     /locus_tag="RHZ76_001013"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             17366..18178
                     /gene="hcaB"
                     /locus_tag="RHZ76_001013"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HDV2811691.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18188..19390
                     /locus_tag="RHZ76_001014"
     CDS             18188..19390
                     /locus_tag="RHZ76_001014"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HDV2811692.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIIGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            19487..19909
                     /locus_tag="RHZ76_001015"
     CDS             19487..19909
                     /locus_tag="RHZ76_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDV2811693.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(19957..20829)
                     /locus_tag="RHZ76_001016"
     CDS             complement(19957..20829)
                     /locus_tag="RHZ76_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HDV2811694.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNHFVWQGREYQLQPNVEWDAHYLHGDGWLGQWQCVSRNDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(20841..21935)
                     /locus_tag="RHZ76_001017"
     CDS             complement(20841..21935)
                     /locus_tag="RHZ76_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDV2811695.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFTLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(21968..22966)
                     /locus_tag="RHZ76_001018"
     CDS             complement(21968..22966)
                     /locus_tag="RHZ76_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2811696.1"
                     /translation="MSASSLSLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(22991..24501)
                     /locus_tag="RHZ76_001019"
                     /pseudo
     CDS             complement(22991..24501)
                     /locus_tag="RHZ76_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
     gene            complement(24524..25369)
                     /locus_tag="RHZ76_001020"
     CDS             complement(24524..25369)
                     /locus_tag="RHZ76_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDV2811697.1"
                     /translation="MRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDA
                     IILSAVSENGSSRTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGN
                     AAADYFIANKIDQPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDK
                     AISVGEKLIISTPDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELE
                     NNQVLKAVVDISGKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATH
                     VDGLP"
     gene            complement(25385..25507)
                     /locus_tag="RHZ76_001021"
     CDS             complement(25385..25507)
                     /locus_tag="RHZ76_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDV2811698.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDT"
     gene            complement(25604..28885)
                     /locus_tag="RHZ76_001022"
     CDS             complement(25604..28885)
                     /locus_tag="RHZ76_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HDV2811699.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQHSLRTCPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNQEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            29003..30196
                     /locus_tag="RHZ76_001023"
     CDS             29003..30196
                     /locus_tag="RHZ76_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDV2811700.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESHTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDHTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVSTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(30394..31647)
                     /gene="glyA"
                     /locus_tag="RHZ76_001024"
     CDS             complement(30394..31647)
                     /gene="glyA"
                     /locus_tag="RHZ76_001024"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDV2811701.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            31975..33165
                     /gene="hmpA"
                     /locus_tag="RHZ76_001025"
     CDS             31975..33165
                     /gene="hmpA"
                     /locus_tag="RHZ76_001025"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HDV2811702.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33210..33548)
                     /gene="glnB"
                     /locus_tag="RHZ76_001026"
     CDS             complement(33210..33548)
                     /gene="glnB"
                     /locus_tag="RHZ76_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HDV2811703.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(33609..34943)
                     /gene="glrR"
                     /locus_tag="RHZ76_001027"
     CDS             complement(33609..34943)
                     /gene="glrR"
                     /locus_tag="RHZ76_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HDV2811704.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(34933..35646)
                     /gene="qseG"
                     /locus_tag="RHZ76_001028"
     CDS             complement(34933..35646)
                     /gene="qseG"
                     /locus_tag="RHZ76_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HDV2811705.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYRSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(35811..37238)
                     /gene="qseE"
                     /locus_tag="RHZ76_001029"
     CDS             complement(35811..37238)
                     /gene="qseE"
                     /locus_tag="RHZ76_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HDV2811706.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLHNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
BASE COUNT         8853 a        10342 c         9488 g         8920 t
ORIGIN      
        1 ccgccaaaat caggcggttt tttgttgctg gtccggttcg cggcctttcc agcaggttgt
       61 attaccgtag taatgcaagc gcgtctcagc ggagacaata ctcgccagta actctctttt
      121 tgtcaagcaa aagagagtta atattgttct gttagtgtat tatccactgc ggccctttcc
      181 gccgtctcgc aaacgggcgc tggctttagg aaaggatgtt ccgtggccgt aaatgcaggt
      241 gtttcacagc gcttgctatc gcggcaatat cgccagtggt gctgtcgtga tgcggtcttc
      301 gcatggaccg cacaatgaag atacggtgct tttgtatcgt acttattgtt tctggtgcgc
      361 tgttaaccga ggtaaataat aaccggagtc tctccggcga caatttactg gtggttaaca
      421 accttcagag cagcaagtaa gcccgaatgc cgccctttgg gcggcatatt ttagattatc
      481 cgattctgtt taaagtcacg caaaaaacca ccccagcgac gttcatagaa tggcgcaata
      541 tgttcggtaa taaagtggct aattcctttt tccccttttt tcacctgaca aatatcgatt
      601 ggttcatcgc caggtaatgt atcggtcgct acacttcccg tcgcctgaat aatttcttcg
      661 atatcaccat cggcttcaat gccaataagt aaattaggct gtgcctcttc gttctcttta
      721 attgaacaaa taaaagcacg cttcaccggc ttaatggttt taaataaggt ggtgagtgaa
      781 tcaatcattt gtgctggcgg ctctgcgact tccgataata tcagcgattc accgccttcc
      841 aggatttcct ggctgctcag cggatttccc tcttcaccaa tcaacaaact gatttcacgc
      901 ggcataaatt ctttaccggt tggcagtttg gcattaagga agagcgtttc gccaagtgtc
      961 atctcaaaca gcgtgcgaac gggcattacg acaaatgcct gttcgtcttc aaccgcctgt
     1021 tgaagtgctt ctaacgaggt gaaaaaagga atgacgctgg tgccgtcttc tttttcccag
     1081 tgctgtaaat caagcgcgct atcttcaacc acagcctcgc cctgcgccgc cgtaccaggt
     1141 acccagacgg tggattccag tagagtacgg aaaaaggccg ggcggtgcgc cggttcagtt
     1201 gctgcttttt ccagcaggtc ttcaagttcg ttttttgttt cggacataag aaccacaatt
     1261 cattcaacgt tcggggcgca aatgcctgat gcgctacgct tatcaggcct acaagggatt
     1321 cgcaatttgt tgaatttgca gaatttgtag gccggataag gcgtttacgc cgcatccggc
     1381 atattagttt acgccgttaa cagattagct atcgtgcgca caccaagtcc cgtagcgccc
     1441 gcagaccact gttcaaccgg cgctttacgg taagtcgccg agcagtcgat atgcatccag
     1501 ccttgctgat agttctcaac aaagtgcgac aggaagcccg ccgccgtgct cgcgcctgcc
     1561 ggatacgccg cgcttccggt attgttcagt tcggcaaagt tagacggcag ctggctgcgg
     1621 tggaactccg ccagcggcag acgccagaac ggttcgttct cctgcgcggc actcgccagc
     1681 aagcgaccgg ccagcgcatc gtcaaaactg aacagcgcgt gataatcatt acccagcgca
     1741 gttttcgccg ccccggtgag ggtcgccgca tcaatgatca tttccggttt ctgcgcactg
     1801 gcatcaatca gaccatcggc aagcaccaga cgcccttcgg catcagtgtt catcacttca
     1861 acttttttac cgttgcgata ggtgatgata tcgcccagct tgaacgcatt gccgctaatc
     1921 aggttatccg cacagcagag gaacagcttc acgcgcttgt tcagtccgcg cgtaatggca
     1981 aatgccagcg ccccggtaac cgttgccgcg ccgcccatgt ccgacttcat cgagtccata
     2041 aacgcagtct gtttgatgct gtagccgccg gagtcaaaag tgataccttt acctaccagg
     2101 cacgcgtaca ctggcgcttc tttatcgcca gttgggttgt aatccagcgc cagcaatacc
     2161 ggagaacgtt ctgaaccgcg tccgacggtg tgcagcccca tataaccttg ctcacgcaga
     2221 tcttcgcctt tggtgatccg ataagtcaca cgatcgcccg cgacgttgct gatcagatca
     2281 acagcacgct gtgccagttg cgatggtccc aattcttctg ccggtgcgtt gatggtgtca
     2341 cgcacccagt cgatgatcat caggcggtta tccagttcct ggcgctgggc atcgtccaga
     2401 tccggccact ccactttacg cgtgcctttc ggggctttgt aaccttgcca gaatgcccag
     2461 cagcgatccg catcccaacc ttcaccgctt aactgaacat gcttgatgcc cagaccgtca
     2521 atcttgcggg ccgcacgctg gatcagcccc agatcgtctg ccccgttcaa atgcagggta
     2581 atgccatcat tattaatgct gtaagttgct ttttctcccc agcgcgcgtc ggcaggttgg
     2641 gtagagaggg taattttcat cgcttctgtc attttagtta tccttattcg tttaacagca
     2701 aaacgggcta ccccaggcag cccgtttatt cattcggcct cgtccagcca gactaacaaa
     2761 atcgcttcga ggattttctc gttggatgcc tgcgggtcgt cgtcgaaatc ttccagatcg
     2821 caaatccact gatgcatatc ggtgaatcga accgttttcg gatcaagatc gggatacgca
     2881 tcgtacagtg cttcgccaat ttcgcggcta tcggtccact taagtcccat actaacctct
     2941 gttaatgctc acgcgcatgg ttgatagtgt aacgcgggat ttcgactact aaatcttcgt
     3001 cggtaacgcg cgcctggcag cttaaacggc tttccggctc cagtccccag gctttgtcca
     3061 gcatgtcgtc ttcctgctct gagctttccg gcagtgagtc aaaaccttca cgaacgatgc
     3121 agtggcaggt ggtgcaagca caggattttt cacaggcgtg ttcaatctcg ataccgttac
     3181 gcagagctgc gtcgagaatg gtttcaccgc tattagcttc cagaacagcg ccatcagggc
     3241 agagatcctg atgaggcaaa ataacaatct ttggcatatt aaacctcgtc cacggaatgg
     3301 cctttcagcg cacgacgaac cgactggtcc atgcggcgag cggcgaaatc ctgggtttgt
     3361 ttgtctacgt ttttaatcgc ttgttcgatg gcgtcaacat catcgccctg cgccacttca
     3421 ctcaggtgag cggcagcatc gtcaatgacc tgacgttctg cggcgcttaa cagcgcggca
     3481 tcagcagcca gcgcgccgtg cagactttcc agcacacgcg ccgcttctac tttttgttct
     3541 gccagcattc gggcttttac gtcctgctcg gcatagctca ttgagtcttt gatcatcgaa
     3601 gcgatttcgc tatcggtcag accgtaagac ggtttgacct gaatagacgc ctcaacgccg
     3661 gtggatttct ccatcgccgt cacgctcaaa agaccgtcgg catcgacctg gaacgtcacg
     3721 cgaatatgcg caccgccagc cggtagcgcc ggaataccac gcagcgcaaa acgcgccagt
     3781 gagcggcagt cctgcaccag ttcgcgctca ccctgcatta catggataga catcgccgtc
     3841 tgaccatctt taaaggtggt gaaatcctga gcgcgggcca ccggaatagt ggtattacgc
     3901 ggaatcactt tctccaccag gccgcccatc gtttcgaggc ccagcgacag tgggatcaca
     3961 tcaagcaaca gcatttcgct gtctggcttg ttacccacca gaatatccgc ctgaatcgcc
     4021 gcgccaatag cgacgacttt atccgggtcg atggaagtca gcggtggacg accgaaaaat
     4081 tcgcctaccc gttcacgcac cagcggcacg cgagtagaac cgcccaccat caccacttcc
     4141 agcacttcat cagcttctac acccgcgtct ttcagcgcgc gacgacaagc cagtaaggtt
     4201 cgttttacca gtggcgcgat cagttcattg aattgttcac ggctgatttc gccctgccag
     4261 cccgcaacgt taacggtcac ggagtccgca tcgctcagcg cgattttggc tgcaatggcg
     4321 gcatccagca gttcacgctg aacgcggtta tcgctacgat caggaatgcc cgcctgctcg
     4381 cgaatgtaat ccgccagcag atggtcgaaa tcatcgccgc cgagcgcgga atcaccgccg
     4441 gttgccagca cttcaaacac gccgcgactt aagcgcagaa tggaaatatc aaacgtcccg
     4501 ccaccgaggt cataaacggc gatcacgcct tcctgaccgg aatccagccc gtaggcgata
     4561 gccgcagcgg tcggttcgtt aagtaagcgc aggacgtgaa ggcccgccag acgcgccgcg
     4621 tctttggtgc cctgacgctg ggcatcgtca aagtacgccg gaacggtgat aactacacca
     4681 tccagctcgc ctgccagggc ttcagttgcc cgcgccgcca gtgctttgag gatgtccgca
     4741 gaaacgcgca ccgggttcag cagccccgcc gccgtttcaa tcatcggcag gccgttttcg
     4801 ctggcctgga attgataagg cagatgcgga tagcgttgct ggatatcagc cagcgagcgt
     4861 cccatcaggc gtttaacaga actaattgtg ttggcggtat cgagcgctgc attagtacgc
     4921 gcgtcataac ccaccgaatg cccttgctgt tgatagtgaa caacagatgg cagcaggtga
     4981 cggccttcat gatcggctaa cgtttcggcc tgaccgctgc gcactgtcgc caccagcgag
     5041 ttggttgtgc ccaggtcaat accggccgcc agacgacgct gatgcggcgc agcactcaaa
     5101 ccaggttcac taatttgtaa taaggccatg tttagcttcc agaaattaaa aatcgagcag
     5161 tttttcttcg agttgttcgg cactgcttcg cagtttatcg agaaaacgca gcttacgcac
     5221 ggtatccgcc gccgcgtccc acgtctcgtt gtctaactgt tcaaccatca actgatggcg
     5281 ggtatcaaac atctttttca cacgtttgat aaagctttcc agccgcgctt catctttcgc
     5341 ctgttcgatc tcgtccagct cttcgcgcag ctccaactgt tccatcagga acgcggtgtc
     5401 gcgcacagta tgctgctcgc tggcgagatc aaagccgtgc aaagaaagca aatattccgc
     5461 gcgcattaac ggatgacgca gcgtttgcca ggcctggtta atggttgcag attgctgtac
     5521 ggcggcgagt tgttccgcct ggcttccgct ggcgaattta tcaggatgat actgacgttg
     5581 tagatcctga aaacgcaggc tcagcgcctg ggtatcgagt tgatagcggg caggcaagcc
     5641 aaagagggtg aagtaatcca taacattctc agggctgcga tccgccgcga taacggcgaa
     5701 tcaggtaaaa caaaccccac gcgcaggcga ccacggtggg gttatcggta tgcgcatcaa
     5761 acgtggaagc tttcgccgca accacactca tctttgacgt tcgggttggt gaatttaaac
     5821 ccttcgttca ggccttcttt tacgaagtcc agctgcgtac cgtccagaaa ttgcaggctc
     5881 ttgccatcga ccacgacttt cacgcctttg tcttcaaaca cgatgtcttc cggcgtcggt
     5941 tcgtcaacaa attccagtac ataagccata cctgaacacc cggaggttct cacgcccaga
     6001 cgcaggccaa accctttacc gcggttagcc agaaaggtat ttactcgcgc tgctgcactg
     6061 tcgctcagtg taatcgacat aaccaaacct caactcttat tttgcttcac gtttgctttt
     6121 atagtccgca atggcggctt tgatcgcgtc ttctgccaga atagaacagt gaattttcac
     6181 cggcggcagt tcaagttctt cagcaatatc ggtgtttttg atcgcctgcg cttcgtcgag
     6241 agacttccct ttcacccatt cggtgaccag ggagctggaa gcgatagcgg aaccgcagcc
     6301 gtaagtttta aaacgcgcgt cttcaatgat accttcatcg ttgactttaa tctgcaactt
     6361 catcacgtcg ccacaggccg gtgcacccac catgccgctg ccgacgttct cgtcgttgtt
     6421 gtcaaaggaa cccacgttac gcggattctc gtaatggtcg ataacttttt cgctgtaagc
     6481 cattataaat tctcctgatt ccgataccga ttaatgatga gcccattcga tgctgttcag
     6541 atccacgccc tgcttgtaca tttcccacag cggagaaagg tcacgcagac gaccgatgga
     6601 tttacgaact aactcgatgg tgtagtcgat ctcttcttca gtagtaaaac gacctaaaga
     6661 gaaacggata gagctatgtg ccagctcgtc gttcagcccc agcgcgcgca gcacgtagga
     6721 cggttcgagg cttgctgacg tacaggcgga accggaagaa accgcgaggt ctttcagcgc
     6781 cataatcagc gactcacctt caacgtagtt gaagctgacg ttgagaatgt tcggcgcacc
     6841 gtgttccagg tcaccgttca ggtaaacttc ttcgatatct ttgatgccgt tccacagacg
     6901 gttacgcagt ccgcgcagac gttccatctc ggtcgccatc tcttcttttg cgatgcgata
     6961 ggcttcgccc attccgacga tctggtgaac aggcagagtg ccggaacgca taccgcgttc
     7021 gtgaccgccg ccgtgcattt gcgcttcgat gcgtacgcgc ggtttacgac gcacatacag
     7081 cgcaccaata cctttcgggc catagatttt gtgaccggag aaagacatca ggtcaacttt
     7141 caactggctc agatcgatag gcagtttgcc cacgctctgg gttgcatcaa cgtgatagat
     7201 aatgccacga gcacggcaca tttcgccgat agccgcgata tcctgtacca cgccgatttc
     7261 gttattcacg tgcatgatag aaacgaggat ggtgtcgtca cgcatcgctg cttcaagttc
     7321 tttcaggtcg ataatgccgt tacgctgcgg tgccaggtag gtgacttcaa aaccttcgcg
     7381 ctccagctga cggcaggtat ccagtaccgc tttgtgttca gttttgctgg tgatgatgtg
     7441 cttgcctttt ttctgataaa agttggctgc acctttgatc gccaggttgt cagattcggt
     7501 tgcaccagag gtaaagacga tttcacgcgg atcagcgccg accagatcgg caatctgatt
     7561 acgggcgata tctaccgctt cttcagcctg ccagccgaaa cggtgagaac gggaggccgg
     7621 gttaccaaag gttccgtcca tcgtcataaa ctgcatcatt ttctcggcaa cacgcgggtc
     7681 caccggcgtg gttgcggagt agtcgagata aatcggtaat ttcattgctc tataaactcc
     7741 gtacatcact caatgcaagg aatcaggcta ccggctggat gtacgaccgt gtttacggag
     7801 cgcgatacgc cccggcctga ttctaatatc ttgttatctt taacctaact gtttcgggtt
     7861 tcagcaagtt gacaaactta cgaatctccg ggagcttaca taagtaagtg accggagtga
     7921 gtatgtgcag tcaacgcaga tgcaacacga aacaggacgg ttaaattaag cgcgtaactt
     7981 aacgtcgatc gcgtcttgta tgcgggtgcg tggcgcgtcg tgagtatgct gacgaccaga
     8041 cacatccagc acttcctggt tattaaccag ttcgcctaaa gtaatgttgt tgagaaaacc
     8101 ggtgagacgg tcgctcaaat cacgccacag cgcgtgggtc aggcatttat caccgccctg
     8161 gcagccgcct ttaccctgac aacgagtagc atctacagat tcgtcaacgg cgctaatcac
     8221 ttcgccaacg gcgatgctgc tggcatcttt gcctaacaga taaccaccgc ctggtccacg
     8281 tacgctggaa accagaccat ttttacgcag acgggaaaac agttgttcca gataagaaag
     8341 ggaaattccc tgacgttcgg aaatatcagc caacggtacc gggcccgctt cagagttgag
     8401 cgcaacgtca agcattgcgg tcacggcata gcgcccttta gatgtcagtc tcatgtctta
     8461 cttcacctca aactcgcccc tgcccggggt tttttattgt aaaagtgggg gtattgcata
     8521 gcagggtcaa gtctgacatt cccgagtaaa ttggtcaact atttacttga ctgatttagt
     8581 cgggtattta accttcagtg ccattttttt atcgtggtgt ttgtacctgt tgtcggatgc
     8641 ggcgtgaacg ccatatccgc cctgggactc tgtgcactgt aggcctgata agacgcgtta
     8701 cgcgtcgcat caggcaacca ctgttattcg gtcttattac ctttattctg ctgctctata
     8761 gaagccagaa tcccgcgcag gatattcaat tcctggcttt ccgggcgcgc acgggtaaac
     8821 agacggcgca atttattcat cacctgcccc ggatggtttt cacggataaa accggttgcc
     8881 agcagcgttt gttccagatg accataaaaa cgctccagat catcgaccag cggatacggc
     8941 gtctcttcat gctcgacctg ctcgccattt tcctgagttg ccagccaggc catgcgcact
     9001 tcataggcaa taacctgaac cgccatcgcc aggttcagcg agctgtattc cgggttagcc
     9061 gcaatcgcga catgataatg gcatttctgc aactcttcat tggtcaggcc gacacgttcg
     9121 cgaccaaaaa ccagcgccac cggggtattt gccgcttcag caacgctttt caaaccgcat
     9181 tcgcgcgggt cgagcatcgg ccacggcagc gtgcgggaac gtgcgctggt gcccaccacc
     9241 aggctacaac cggctaacgc ttcgtcgagc gtatcgacga tgtgagcatt accaatcaca
     9301 tcgctggccc ccgccgccag ggcaatcgcc tgggagtcag gtttcaccag tggattaacc
     9361 agccacagat tggttaatcc cattgttttc atggcacggg caacagaacc catattgccg
     9421 gtgtgtgacg tctccaccag cacaattcga atattttgca gcattgtctt tcttcgtcta
     9481 aagattattc gcgcatctta tcataaaacg aagacagatg ccgatctcgc tgctatactc
     9541 tgcgccgttt tcccgttctt taacatccag tgagagagac cgatgcatcc gatgctgaac
     9601 atcgccgtgc gcgcagcgcg caaggcgggt aatttaattg ccaaaaacta tgaaaccccg
     9661 gacgctgtag aagcgagcca gaaaggcagt aacgatttcg tgaccaacgt agataaagct
     9721 gccgaagcgg tgattatcga cacgattcgt aaatcttacc cacagcacac catcatcacc
     9781 gaagaaagcg gtgaacttga aggtactgat caggatgttc aatgggttat tgatccactg
     9841 gatggcacta ccaactttat caaacgtctg ccgcacttcg cggtatctat cgctgttcgt
     9901 atcaaaggcc gcaccgaagt tgctgtggta tacgatccta tgcgtaacga attgttcacc
     9961 gccactcgcg gtcagggcgc acagctgaac ggctaccgtc tgcgcggcag caccgctcgc
    10021 gatctcgacg gtactattct ggcgaccggc ttcccgttca aagcaaaaca gtatgccact
    10081 acctacatca acatcgtcgg caaactgttc aacgaatgtg cagacttccg tcgtaccggt
    10141 tctgcggcgc tggatctggc ttacgtcgct gcgggtcgtg ttgacggttt ctttgaaatc
    10201 ggtctgcgcc cgtgggactt cgctgcaggc gagctgctgg ttcgtgaagc gggcggcatc
    10261 gtcagcgact tcaccggtgg tcataactat atgctgaccg gtaacatcgt tgctggtaac
    10321 ccgcgcgttg ttaaagccat gctggcgaac atgcgtgacg agttaagcga cgctctgaag
    10381 cgttaatgac tcaggcgggt gatatcactc acccgccctc gcctttcagg cgctattccg
    10441 aaatacttcc tcactgcttt actttctttc gtcactctcc caccatcttt ccccgattaa
    10501 tggatgataa agaagtcgca aggaaaggtt atggcactgc cagtgaacaa acgcgttctc
    10561 aaaattctgt ttattctctt tgttgttgcc ttctgcgttt atttagtgcc gcgcgttgcc
    10621 atcaacttct tctattatcc cgacgataaa atttacggtc ccgatccctg gtcggcggaa
    10681 tccgtcgaat ttacggctaa ggacggtact cgtctgcaag gctggtttat cccttcttcg
    10741 tcgggccctg ctgacaacac catcgcaacc atcattcatg ctcacggcaa tgccggaaat
    10801 atgtccgccc actggccgct ggtcagttgg ttacccgagc gtaatttcaa cgtttttatg
    10861 tttgattatc gcgggtttgg taaatcaaaa ggcacgccgt cccaggccgg attgctggac
    10921 gatacgcaaa gcgccatcaa tgtggtgcgc catcgcagtg atctaaaccc acaacggctg
    10981 gtgctgttcg ggcagagtat tggcggggcg aatattcttg ctgttattgg tcagggtgat
    11041 cgtgaaggca tacgtgcggt gatcctcgac tccacatttg cctcttatgc aaccatcgcc
    11101 aaccagatga tccccggcag tggctactta cttgatgaga gttacagcgg cgaaaattac
    11161 atcgccagcg tcagcccgat cccgctttta ctcattcacg gtaaagccga tcacgttatc
    11221 ccatggcagc acagcgaaaa gttgtatagc ctggcaaaag agccaaaacg gttgatccta
    11281 atcccggatg gcgaacacat tgatgctttt tccgatcgtc acggcgatgt ttatcgcgaa
    11341 cagatggtgg actttatcct tagcgcgttg aatccgcaga actaacccat gatcgctagc
    11401 acgataataa ttcacaaaac caccttaaga catgctaatc cactggtcag aacagtttaa
    11461 gatgagaaaa attctgtgac gcttgccaac atttctgatg attagcattc ccttcgtcat
    11521 ttccctgagc aaactttagc tattcttatc aattatgctt atgggagatc tacagatgat
    11581 gcctacgctt gctccaccat ctgtcctttc ggctccccag cgccgctgcc agatcttgct
    11641 gacgctcttt cagccggggt tgaccgccac catggcaacc ttcagcgagc ttaatggtgt
    11701 ggatgatgat attgccagtc ttgatatcag cgaaacagga cgggagatcc tgcgctatca
    11761 tcaactcaca ctgacgactg gttatgacgg tagctaccgg gttgaaggta cagtgcttaa
    11821 ccaacgtttg tgtttatttc actggctacg acgtggtttc cgtctgtgtc cgtcatttat
    11881 taccagccaa ttcacccccg ccctgaagag tgaattgaag cggcgcggaa ttgcgcgtaa
    11941 cttttacgac gataccaatc tacaagcgtt agtgaatctc tgctcccgac ggctgcaaaa
    12001 acgctttgaa tcgcgcgata ttcatttcct gtgtctgtat ctgcaatatt gtttgctgca
    12061 gcaccacgct ggaatgacgc ctcagtttaa tccgctccaa cgtcgctggg cggaatcctg
    12121 ccttgaattt caggtagcgc aggaaattgg acgccactgg cagcgtcggg cgctccagcc
    12181 tgtaccgcct gatgagccac tgtttatggc actacttttt tccatgttgc gggttcccga
    12241 tccactgcgg gatgcgcatc agcgagacag acaattgcgt cagtctatca aacgtctggt
    12301 aaaccatttt cgtgagctgg gaaatgttcg tttttatgat gaacaggggt tatgtgatca
    12361 gctttatacc cacctcgccc aggcgttaaa tcgcagtttg tttgccatcg gtattgataa
    12421 taccctgccg gaagagttcg ccagactgta cccacgcctg gtgcgcacca cccgcgcggc
    12481 gctggccgga tttgaaagtg aatacggcgt ccatctttct gatgaggaaa gtggtctggt
    12541 cgcggtgatt ttcggtgcct ggctaatgca ggaaaacgat ctgcatgaaa aacagattat
    12601 tctactgacc gggaatgata gcgagcgaga agcgcagatt gagcagcagt tacgcgaact
    12661 aacgttactg ccgctcaaca ttaagcatat gtcggtaaag gcatttttgc agacaggcgc
    12721 tccgcgtggc gcggcactga ttattgcgcc ttataccatg ccattaccgc tcttttcacc
    12781 accgctgatc tatacggacc tgacgctgac aacacatcaa caggagcaga tccgcaaaat
    12841 gctcgaatca gcatgaggga acaactttcg ggcgcaaaaa cattgccggc agcgccacca
    12901 gcgccattac ccagaacacg ccgtggccca gatattgata caggaaaccg gcgaaaacgg
    12961 tcatgatagc gatactgccg cccatcgcga cggcagagta aaccgcctgt aaacggatga
    13021 cttcgctacc ctggcgagca gcaatataac gcatggcggc caggtggcag accgtgaagg
    13081 tgccgcaatg cagaatttgc accactatca accacggcaa cgccgtagtt gctcccataa
    13141 tgccccagcg cactacgccg caaatcgccg agatcaacag catatcgcgt gcactacaac
    13201 ggcggaaaag tttattactc agcgcaaaga taatgacttc cgccaccacg cccagcgacc
    13261 acaaataccc caccgccgag gccgagtagc cagctgcctg ccagtaaatg gcgctaaaac
    13321 cgtaataggc cgcatgtgcc ccctgcaata aacaaacgca ggccagaaag cgccagttct
    13381 ggcgaaccag cgccaaccac gctgaccaac cggtgctctc ctgctggcgg cttgcccctt
    13441 gtggctgaat cgtcggacgg atgagaaagc cgagcagcat ggatgccacg cccaacgtca
    13501 acagcgcgag gatcacccga taatcaaaca tagtgaccag tttgcccgtc agcgccgagc
    13561 caatgacaaa cgccaccgag ccccacagtc gcactttgcc gtaatcaagc gggaactgct
    13621 tttgccacgt attcgccagt gcatcggtca acggtaccag cggtgagaaa aagaggttaa
    13681 agccaatcat caccagcatc agccacgcta cgtgcgcccc cgcccagaag gcgacagcaa
    13741 agagaagtgt cagcagtgcc agcacgcgca aggcgaaaat caggcgggaa ggatcgctga
    13801 cgcggggcgc gatgagcaaa ctcccgagaa aacgggcaac cagacctgcc cccaataaca
    13861 ggccgatggt ttctggcgtt aaaccaatcc ctttaagcca gacgctccag aaaggtagaa
    13921 aaatgccgta actaaaaaag tatgtgaaat agccgagcgc caaccagcgc gtggattgca
    13981 aaaccatgag tccctcccgt caggaggcgt tagtctgggg ttaatcgaat gttggtgcaa
    14041 gttgaaaaat aagaatattc gagcctgctt cccgatgtct gtaacgccag ttcacaagca
    14101 ctaaaaaact gagatccgcg acgcatgttg caaaaataat gcgtaattat gccgttacgc
    14161 ttgccaaacg ttcctgcaca atggcgatga aatcgcgtaa tgccggcttc atctcccctt
    14221 tcttccacgc catcagtaaa gcaatggaag gcacatttcc ggcaatggga cgaaaaacaa
    14281 cctgcccggt attaaaatta ttcatataac cgggtatcaa agtgacgccc agccccatgc
    14341 ccaccagatt catggtcacc agaatattcg ttgccacctg gacgatattt ggctggctgt
    14401 tttcttgcgc aaaccacgct ttaacgatcg gcgcaagcga accggaatac gccggatcgg
    14461 tactgacgaa attcacgcca tccagttgcg cggcggtgat ctctttttca tgtgctaaag
    14521 ggtgatcaac cggtaacacc accactaatg gttcgtcaaa aagctccaga taatcaatct
    14581 ccgggctata aacaggatgg cgcattaagc cgacgtcgag ttcaccacgg cgaatttttt
    14641 cctcctgttg cgtggtgatt aaactcacca gctcaatcaa ggtgtctggc tgtctgagac
    14701 gaaacatcgg taatactttt ggtaataaat tcacttccgc cgatggcaca aagccaatgg
    14761 ttaattgtct gtcttcctga acaattttcc gcgcccgtaa tttggcattt tccgcttgtt
    14821 cgaggattgc cagcgcgtcc tggagaaaac attctcccgc cgccgtcaac gcgactttgc
    14881 gcttatccct caccagtaac ggaacaccga cacagttttc aagatcgcgg atctggctgc
    14941 ttaacgaagg ctgtgaggta tgcagttttt ccgccgcacg ggtaaagttc agtgcctgcg
    15001 ccactgcgac gaaatagcgt aaatgccgta gttccatcac cttccccttg ttatcgaaaa
    15061 aacgtctcag ctggtagaaa ttaactattt cacatattag caaccaacca gcaacatcct
    15121 tatggcacaa aaatagaagg tcaatacatc ttatctttca ggattaaaaa atatgaccac
    15181 accctcagat ttgaacattt accaactgat tgacacccaa aacggtcggg tcactccgcg
    15241 tatttatacc gacccggaca tttaccaact ggagcttgag cgtattttcg gtcgttgctg
    15301 gctatttctc gcccacgaaa gccagatccc aaaacccggt gatttcttta acacctacat
    15361 gggagaagat gcggttgtcg tagtgcgtca gaaagacggc agcatcaagg cgtttctcaa
    15421 ccaatgccgc caccgggcca tgcgtgtgag ttatgcagat tgcggcaaca ctcgcgcctt
    15481 tacctgcccg tatcacggct ggtcttatgg cattaacggc gagttgatcg atgtaccgct
    15541 ggaacctcgc gcctatccac aagggttgtg taaatcccac tgggggctaa acgaagttcc
    15601 ttgtgtggag agttataaag ggctgatttt tggcaactgg gataccagcg caccgggcct
    15661 gcgtgattac ctgggtgaca ttgcctggta tctggatggc atgctggatc gtcgcgaagg
    15721 cggcaccgaa attgtcggcg gcgtacaaaa gtgggtgatc aactgcaact ggaaattccc
    15781 ggcagagcag ttcgccagtg accagtatca tgctctgttc agccatgctt ctgccgttca
    15841 ggtattaggg gcgaaagatg atggcagcga taagcgcctc ggtgatggac aaaccgcccg
    15901 cccggtgtgg gaaaccgcca aagatgcgct gcaatttggt caggacggtc acggtagcgg
    15961 tttcttcttt actgaaaaac cggatgctaa tgtctgggtc gatggcgcag tttcaagcta
    16021 ttaccgcgaa acctatgccg aagcagaaca acgtttaggt gaagttcgcg ccctgcgcct
    16081 ggcgggtcat aacaatattt tccccacgct ttcatggctc aacggcactg ccacgctccg
    16141 cgtctggcat ccgcgcggcc ctgatcaagt tgaagtgtgg gcgttctgta ttactgacaa
    16201 agccgcctcc gatgaagtta aagccgcttt tgaaaacagc gccactcgtg cttttggtcc
    16261 tgctggtttt ctcgagcagg atgactcgga gaactggtgt gaaatccaga aattgcttaa
    16321 aggccaccgc gcccgcaaca gcaaactgtg tctggaaatg gggcttggtc aggaaaagcg
    16381 tcgcgacgac ggcattcctg gcattactaa ctatattttc tcagaaactg ccgctcgcgg
    16441 aatgtaccaa cgttgggccg atctcctgag tagcgaaagc tggcaagaag tgctcgataa
    16501 aaccgccgct taccagcagg aggtgatgaa atgagtgcgc aagtttcact ggagttacat
    16561 caccgcatta gccagtttct ctttcacgaa gccagtctgc tggatgactg gaaatttcgt
    16621 gactggctgg cacagctcga cgaagagatt cgttacacca tgcgcaccac ggttaacgcg
    16681 caaacgcgcg accgccgcaa aggcgttcag ccaccgacga cctggatttt taatgacacc
    16741 aaagaccagc tggagcggcg aatcgcccgt ctggaaacgg gcatggcctg ggcagaagag
    16801 ccgccgtcac gcactcgtca cttaatcagc aactgccagg taagcgaaac cgacatccca
    16861 aatgtatttg ccgtgagggt gaattatcta ctttatcggg cacaaaaaga gcgcgatgaa
    16921 acattctatg ttggaacgcg tttcgacaaa gttcgccgtc tggaagatga caactggcgc
    16981 ttgctggaac gggatatcgt cctggatcaa gcggtaatca cttcccataa cctgagtgta
    17041 ctgttctgat gaatcgaatt tatgcgtgtc ccgttgcgga tgtgccggag ggtgaggctc
    17101 tccggatcga tacctcgccc gtcatcgccc tgttcaacgt tggcggcgag ttttatgcca
    17161 ttaacgatcg ttgcagccat ggtaatgcgt caatgtcaga agggtatctg gaagatgacg
    17221 ccacggtgga gtgcccgcta cacgccgcca gtttttgcct gaaaacgggg aaagcgttat
    17281 gcctgcccgc caccgatccg ctcaccactt atccagtaca cattgaaggt ggtgacattt
    17341 tcatcgactt accggaggcg cagccatgag cgatctgcat aacgagtcca tttttattac
    17401 cggcggcgga tcgggattag ggctggcgct ggtcgagcga tttatcgaag aaggcgcgca
    17461 ggttgccacg ctggaactgt cggcggcaaa agtcgccagt ctgcgtcagc gatttggcga
    17521 acatattctg gcggtggaag gtaacgtgac ctgttatgcc gattatcaac gcgcggtcga
    17581 tcagatcctg actcgttccg gcaagctgga ttgttttatc ggcaatgcag gcatctggga
    17641 tcacaatgcc tcactggtta atactcccgc agagacgctc gaaaccggct tccacgagct
    17701 gtttaacgtc aacgtactcg gttacctgct gggcgcaaaa gcctgcgctc cggcgttaat
    17761 cgccagtgaa ggcagcatga ttttcacact gtcaaatgcc gcctggtatc ctggcggcgg
    17821 tggcccgctg tacaccgcca gtaaacatgc cgcaaccgga cttattcgcc aactggctta
    17881 tgaactggca ccgaaagtgc gggtgaatgg cgtcggcccg tgtggtatgg ccagcgacct
    17941 gcgcggccca caggcgctcg ggcaaagtga aacctcgata atgcagtctc tgacgccgga
    18001 gaaaattgcc gccattttac cgctgcaatt tttcccgcaa ccggcggatt ttacggggcc
    18061 gtatgtgatg ttggcatcgc ggcgcaataa tcgcgcatta agcggtgtga tgatcaacgc
    18121 tgatgcgggt ttagcgattc gcggcattcg ccacgtagcg gctgggctgg atctttaagg
    18181 aagcacgatg aaagaaaaaa cgatcattat tgtcggtggc gggcaagcgg cggcaatggc
    18241 tgcggcctcg ctacgccagc aagggttcac cggtgagctg catctgtttt ccgatgagcg
    18301 acatcttcct tatgaacgac ctccactctc gaaatccatg ttgctggaag attccccgca
    18361 gttacagcag gtgttacccg ctaactggtg gcaggaaaac aatgttcatc tgcattccgg
    18421 tgtaaccatc aaaacgctgg gtcgcgacac acgagagtta gtgttaacca acggcgaaag
    18481 ctggcactgg gatcagcttt ttatagcaac cggcgcggca gctcgaccgc tgccgttgct
    18541 tgatgcactg ggagaacgct gctttaccct acgccatgcc ggtgatgccg ccagactgcg
    18601 agaagttctg cagcccgaac ggtcagtcgt gattatcggt gccggaacta ttggtctgga
    18661 actggctgcc agcgccacgc agcgcagatg taaggtgaca gtgattgaac tggcggcaac
    18721 cgtcatgggc cgtaatgcac caccgcccgt gcaacgctat cttttacagc gccaccagca
    18781 agctggtgtg cgcattctgc tcaataatgc cattgaacat gtggtcgatg gtgaaaaagt
    18841 agaactgacg ctgcaaagtg gggaaacgct tcaggctgat gtggtgattt acggtattgg
    18901 tatcagcgcc aacgagcaac tggctcgcga ggccaacctt gatactgcca atggcattgt
    18961 cattgatgag gcttgccgca cctgcgatcc cgcgatcttt gccggtggcg atgtggcaat
    19021 cactcgtctt gataatggtg cactacaccg ctgcgaaagc tgggaaaacg ccaataacca
    19081 ggcgcaaatt gccgctgccg caatgttagg gctaccgcta ccgctactgc cgccgccgtg
    19141 gttctggagc gatcagtaca gtgataactt acagtttatt ggcgatatgc gtggcgatga
    19201 ctggctttgt cgtggcaacc cggaaactca gaaggcgatt tggtttaatc tgcaaaacgg
    19261 cgtgcttatc ggtgcggtca cgctgaatca ggggcgtgag attcgcccaa ttcgcaaatg
    19321 gatccagagc ggcaaaacgt ttgatgcgaa actgctgata gatgagaaca tcgcgcttaa
    19381 atcactgtaa ccaggataat tagcgaatat ctcaacgcct ggggcgtggc gaggtgcaag
    19441 agtgtgtatt acgtttaaat cacattatct tgcaaaggga ttggttatga acacactacg
    19501 ttattttgat tttggagctg cccgccccgt tttgttatta attgcccgta tcgccgtggt
    19561 cttaattttc attatttttg gttttcccaa aatgatgggc tttgacggta cggtccaata
    19621 tatggcctcg ttgggcgcgc caatgccgat gctggcagcg attattgcgg tagttatgga
    19681 agtgcccgcc gcgatattaa tcgtgcttgg ctttttcacc cgtccgctgg cggtgctgtt
    19741 tattttctac acgctgggta cggcggtgat tggtcaccat tactgggata tgaccggcga
    19801 tgcggttggg ccaaatatga ttaatttctg gaagaatgtc agtatcgctg gcgcgttctt
    19861 gctattggca attaccgggc cgggggcaat ttctctcgat cggcgttagg aaagatgccg
    19921 gatgcggcgt gaacgcctta tccggcattt aataaattac agccactcta cccgcaacga
    19981 catctctgaa gttgttgatt cccctggtgc tagcgcaatg agatcacccc cttccgggcg
    20041 atgatgatca tccggcgcat ggctcatcgg ttccagacag aagaaatcaa acgcatatcc
    20101 tttatcaaac gcagggtcgg aaacaaagat gaaataacac ggtgcaggtg gcgtcgtttc
    20161 catgatgatg gcataacctt cctgcggctg ctcgatgcgg gcctgaccat tccatccggc
    20221 aaaaccattg ttcacccact ggcgcggcaa cggcgcgagc tggttaaaat ccagttcctg
    20281 cggtagctgc tcgcaaaact cacccgccag ccactgctcc cgttccagcc agtaaccgct
    20341 cgcctgcgcc tgaatccgcg tttgtggtga caacggaaaa taaggatgcc agccggtacc
    20401 aaatggcagc gtctctgctc cttgattggt gacagagagc gtcaccgtca gcgtatccgc
    20461 cgttaaatga aacgcctgac ttacccgata gtgataaaca ccgctgcgat gttcatacac
    20521 cagacacaag ctatcatcat tgcgcgagac acattgccac tgccccagcc agccgtcgcc
    20581 gtgcagatag tgcgcatccc attcaacatt cggttgcaat tgatactcac gcccctgcca
    20641 gacaaaatgg ttgccgctca cccggtttgc aaacggcacc agcggaaaac aggaggcatc
    20701 ggttgccaca ccgctttttt taccaggacg taagagcggc gtcgtatcgc gccagaagcc
    20761 ttcgattacg ccgccctggt cagaaacgtc cagctttagc gacccgtggg ataaggtata
    20821 gatggtcatc cgctaactcc ttaatccggg aagttaatca caactttccc gcatttgccg
    20881 ctcgccatca gcgcataggc atcacctgcc tgttccagcg tgaagcgatg ggtaatggcg
    20941 ttacgcggcc acagtttcca gtccgtcaaa tcgtgggcgc atttttccat atggaacaga
    21001 ctggtcaccc aggagccgat aatccgccgt tgatggtgca tcagatcggc gctgacctcg
    21061 aactccactt ttccggtttc accaatgtaa accacccgtc cccagtcagc ggtggattgc
    21121 agtgccagca agcgacctgc ggcattacca gaacaatcga gcgcaacatc cgcgccaccg
    21181 tgggtaagtt cagcaattat ctgcggcaga ccttcggtgg tcgctaaata gccgtgatcc
    21241 atcaccccta actgttttgc catcgccaga cgttccggca gcatatcaac gccgatgatc
    21301 cgttttgcac cgcgaccttt cgccagcatc atcgccatca tgccgactgg ccccagaccg
    21361 accaccagca cgttatcact gccggaaact tcgccgcgca aaattccttc atacgctgta
    21421 ccaacgccgc aactgataaa cgcaccatct tcgtagctca gcgcatccgg caggaggatc
    21481 agatcttttt cttccgccag caagtattcg gcatgaccgc cgtcacgctg ccagccgtaa
    21541 gccgcttttc cttcgccagt acaagaaata ggaaaaccgc gacggcagtt cgggcaaaaa
    21601 ccacagccag aaatgtgata caccagcacg cggtcgccct ctttaaaatg gcggcagcct
    21661 tgccccatcg ccacaatctg cccgcatggt tcatgaccgt tgataaatcc ctggtataac
    21721 ggtttatcgg gtgccgccgc cgtggcacgg tgttgatgat agatatagtg gacatcgctt
    21781 ccgcaaatcc cggaggattt cattttgatc agtacctggt taatccccgg cgtcggcacc
    21841 gcaacttccc gcagatcgac ggttgaattt cctggtaaat aagctgccag catcgttttc
    21901 ataaatccct cattaacaat acgattaatt ttcatccctg ccgcacccgc gccaggggcg
    21961 ttaaggttta gcgtttcgct ttactgcttc gctgggtcag caagatattc gccagcaccg
    22021 ccaccacgat gatgacgccg cgtaccacct gctggaaaaa ggagttaata ccgagcagca
    22081 ccagaccgtt accgattagc gtaatcacca gcacaccaag caatgtaccg aacagggaac
    22141 cgcgaccgcc ggaaagtgcc gtaccgccga ccacgaccgc agcgatgacg tcaaactcca
    22201 gaccgtttgc ggcacctgcg ttaccggaac cgaggcgtgc cgccagcaaa atgccggtca
    22261 ccgccgctaa taatcccgaa agggtaaaga taagaatgcg cacccgacga acgttaatac
    22321 cgcacaactg cgccgccgtg gcattaccgc caacggcaaa aaccgagcgc ccgaaggcgg
    22381 tttttcggct aatgaacaca aacagcgcaa acaacaccat catgatcagc gcggatactg
    22441 gcacaccgag aaattgtccg cccagccagt ccagcacctc gttttcgtca atcggcactg
    22501 gcagtgcgtt ggtcataaac agccccattc cgcgcagggc gctccacagc cccagtgtgg
    22561 caacgaaact tggtacgtta aacacgccgc gcagcacccc ggcgagcgtt cccatcagcg
    22621 cgcctaacag caacaccagc agacaagcaa tcgccaacgg aacttcaaat tgcagcaaaa
    22681 atgccaggca caccgagaca aaagccacca tcggcccaac gctgacatca atttcaccgg
    22741 agataataat cagcgtcatc gcccaggcgg caatcccaat ggtggcggca tcgcgcagca
    22801 cgttcatctg gttattcaat gagataaagc caggcgcgtt cagggagaag accagataaa
    22861 gaatggcgat taccaccagc aaaccgatct cattaatatg gcgactgaca aattgtttga
    22921 gcgagacgct cttgccctgc ggcaatgata atgacgaagc agacatcttc aggttcctcg
    22981 gtaaaattca tcagtgcaca gacagaatgg cggacatcag ctcatccaca ttgaccggcg
    23041 agtgaaactc ctgcgagaac gtaccgtgct gtaacaacag gatgcggtcg cacaccagcg
    23101 gcaactcctc cacttcactg gagataaaca ccacgctttt ccttcggcag ccagctcacg
    23161 gacaatacgg taaatctgct gtttggcttc gatatcgacg ccgcgcgttg gctcgtcgag
    23221 caacaaaatc tggctggcgg catagaccca acgaccgatc accacttttt gctgattgcc
    23281 accagaaagc gtgccgatgg gtgtttcgct actggcagcc ttgaccgtca tccgctgcat
    23341 cacctcttcg gtcaggcggc ggatggtgga ccattgcagc acaccgttgg tgctgatttt
    23401 ttgccgattg gtcagcactg tattttcgtc aacgcccagc cagggaatga tccccgcttc
    23461 tttgcggttt tctggcgtat agccaatgcc gcgtttcagc atgtcgccgt aatcggggcg
    23521 catgattttc tcgccgttga taacaatttc gccctgttca tacgtctcca gcccaacaat
    23581 cgccttcagc aattcactgc gccctgcccc cagcaggcca gcaatgccaa gcacttcgcc
    23641 acgacgtagc gtaaagctga tatcctccag cttgggctta tggcgtaacg cacggacttc
    23701 cagtacggcc tgatccacaa tttcctgagg ggctaccggc gcaatatcaa cgtgatcacg
    23761 ccctagcatt agcgacacaa tatgatgcgt ggaggtgttt tcgagcatca catcgcccgc
    23821 cacctgacca tcgcgcataa cggtggcaca ggaggcaatg cggcgaattt cttccatccg
    23881 gtggctgaca taaatcaccg ccacgcccag tgccgacatc tttttcaccg cgctgatcac
    23941 cagttcaact tccgcactcg caagcgaact ggtaggttca tcaagaatga ccacgcgcgg
    24001 ctcgcccttc atcacccgcg caatttccac cagctgcttt tgcgccgggc ttagcgttga
    24061 aacaagttgt tcaggactaa cgtcaacgcc cagcgcctgt aagcaacgtt gggcatcctg
    24121 cgccatttgc aggtaatcaa tcatgccgtt gcggcggggc cactgaccga ggcagaggtt
    24181 ttccgccacc gtcagccctt ccaccagact taattcctga taaaccgcac gaacccccag
    24241 ttcagcggca cggcgagtca acgtagcttc gtcaccttcc agtcgcgtct cgccaatcca
    24301 gatatcaccg ctatccgggc gttcgctgcc ggtaagcatt cgaatgagag tcgatttgcc
    24361 cgcgccgttt ttacctaaca gcgcacgaac ttcgccttta ttgagcgtga agttaacgtt
    24421 atccagcgca acgacgccgg ggtaacgctt atttcctgcc accacttttg ctaccgggac
    24481 tgcctctgtt gccgtgaaca tagggacctc tgcgaatcag cgattagggc agaccatcaa
    24541 cgtgcgttgc cagccactgt ttaccatctt ccgttttggt atagagatcg attggcacct
    24601 gaatcacttt ttcaccgtcg gcttgtttat tgataacctt caatgtttgc gcgaaaacag
    24661 cattgcccat tttcttaccg gaaatatcca ctaccgcttt cagcacctga ttgttttcca
    24721 gctcctgagc aatttcggtt gtcatatccg aaccgaaaac agcaattttt ccggcctgat
    24781 tttgattacg taccgctttt accgcgccga gtgtcgcacc gcccgactcc cccataatgg
    24841 cgttgagatc cggcgtggag ataatcagtt tttcaccaac ggaaatcgct ttatctaaaa
    24901 cagtcccttc ctgattagcg acaatttgcg cgccgggaac gcgggatttt aatacttctt
    24961 caaatccttt acgtcgctgc acacaaactt caaaggcttc gcaattgatg acggcaattt
    25021 tcggctggtc aattttattg gcaataaaat aatcggcggc agcgttaccc agttttttac
    25081 caaattccag tggatcgccg accagatacg ccgagacata tttatcgaca cccttttgat
    25141 taatacaggt gttgtagcaa atcaccggaa tacccgcttc actggcgcga cgaacggtac
    25201 ggctactgcc attttcagac actgccgata aaataatggc atcgacatta cgcgccacga
    25261 gggtatcaac aaaggtactt tctttcgaaa tatcaccctg ggcgttagtt tcaattaact
    25321 gtacctgtac tgaagaatct tttgccgcat cctgaacgcc ctgacgcact ccagcgtaat
    25381 atccctaggt atcaaggtat attgcaccaa tggtcatttc tttatccgca gccctggcaa
    25441 atagtgcgct acctaatagc gtagccatta ataatagatt acgggttgtt ctcatttttt
    25501 taggcatagt gcctcctgta gggtttttat taacaacggc ttattctaat tattttgtga
    25561 tgagcggcag cgcatgccgc tcaaatatta cagttaatag ggtttagtga acgtagttaa
    25621 aaataaaagg catcacggtg gtgaataatg ccgctttcgg ccaggccgga ttaatcgccg
    25681 tcaattcagc gcgagcagat tcatattgcg caacctcccc gagtcccgcg gacgccagca
    25741 tcgccaccat caggcatttt tctttatgct gctgttgtaa atcgccatac agcgacaaca
    25801 ggtcaggttg tgaaacagca aagaaatccg cctcaatact ggttttcgcc atctcttgcg
    25861 cccactgttt catttcacta aacagttgct gtgcggtttg ctgttcaccc agcagtcgca
    25921 gcgccattcc ttgccagaag agataatcaa ccggctgatc gttgtaataa ctgtggatgt
    25981 taatggtgcg atcgccggtc gccgccagac gtaaacaacg catcgcttca gtttcatcgc
    26041 cctgcgcgtt ggcgcatatc gcctgccaga accagatgtc gttatcagtt tgccccggta
    26101 aacggccttc gcttaaattc tccggataat gcagcgcggc atgaagcagt tcgctggcct
    26161 gctgcggctc tctggcatca agatgctgcc aggcgcgtaa taactggttg aggataaact
    26221 gactggtgac cttcccttcc ccgccttccc acgggtggaa tttacgcgtg gcgagaatgt
    26281 ccgccgcttt gtctgcctga cccgtgagat gccacaaatt gagcagttct gcggtcatgt
    26341 cgtcgcgttt cagcgcaatt tcctgattat tttccagacg cgccagtcgt ttctccggtg
    26401 tggctccact taacttatca agcaaatccc gttcgaaaag cagacgtgca tcctgcggcg
    26461 caagctgata agcattatca agataacgcg cggccagctc ataatcgtgt tgcttattcc
    26521 acgcatggat cgctaacccg cgccagccgt cggcaaactc cggcgacatc tctacgcaac
    26581 gttgccataa ggcaatggct ttgttgtagc tacgtttgtt atagtagaag caggccagta
    26641 aatggcgagc aaaccagcac tcttcaatac tctccagcgc cgccacttct tccagcgtat
    26701 tcgggaaacg gacaaactgc gggaagacat caatggcttt tgcgaccagt tcgccacgtt
    26761 cggctttcgg cagcaagctg gcttgcaggt aaagcggcag cgtgcgctgg cagtccagtg
    26821 cgttcagcat ctctgccgcc agggtgggca ttccccagtt aatcagttgc ccggcggtca
    26881 tcagagcgtt aacgtcgcgt ccctgacaca gaccgcgcca ctgcgcgagg gctgattcgc
    26941 tacgaccatc gaaccagttc agccaccaca gagtggcgtt cagcggataa tcgcgcagca
    27001 gtttctcgcg ctgcaaacgc gcgttgtcct gacgaccact taacaccagc agcaggttat
    27061 gcaggcaaag cacttcctga ttggttgggc aggtgcgaag actgtgttgg caaaaatcca
    27121 gacccgcgtc gaagtttcca ttacgcgccg ccagtcgtgc cagaccataa tagccgcctg
    27181 ctttactatt gccgctccag accgcacgcc agaaatcctc ttcggcttgt tgatattgtc
    27241 cctgacgttc gtaagcactg gcgcgaatca aactcgcctg tccgcactgc ggatttttgt
    27301 tcagcgcatg tgcgcgtttc agcgcctgag tggcatacgc caccgcttgt gggaaatctg
    27361 cgcggttata ttccagcatc gccagcgcca ggttacagcg gtaatccagc ggatccagcg
    27421 ccacaccgcg caggtagtaa tcgaacggtg aacggctggc gtgatgatat tgctccagat
    27481 gctgaccgat aaaccaggct tcatctgtac tggtaatgtc ttgtgctgcc agtggcgctt
    27541 tggcgacgtc cggcaacggc aactcttgcg gctgatgttc ctgataactc agtacaatat
    27601 tgccgtcggc atcggagagc tcaatggtca gcctttcagg attgataccg tgcaacacgc
    27661 cctggatggc ggtcgctggt gtcaaggcca cggcatcatc aagtaacgcg ttgcatttgc
    27721 cgatttcgcg gatcgccagg cggtatccgt tcaacggaga gatggcatac agcccccact
    27781 caatcccccg ctcactacgc tggagtttta tcaccgcatc gcgggaggca ttttgcacca
    27841 tgcccagaga atgataaggc aggaaatact gctcgaaacg cttctcttcg taagcatcaa
    27901 gccaggtaaa atcaggctgg ttatcggcaa aaataccggt catcagttcg atatacgggc
    27961 cgttattgtc ggtcaggctc ttgtcccacg cctggccaaa ttcactgtgt ccccaactcc
    28021 actgtttttt acctggcgca atatggtggt tggcaacgtg cagcaaaccg ccatcttcat
    28081 cgtgacacca cgcgccaaca aaatcgtact gtgatttttc agccatatat gaggttggaa
    28141 cgggcacatt tttatagcga gaaatgtcca ctccagcgga gtagtccact ttgtagtaag
    28201 tgccggtggc gatggggaaa gcggagacgg cccgtttgcc gtgatcaaac accgccgtta
    28261 catccggcgg gaaaacgctc tgatgccctt cccccccttt cactgccggg ttggcccacc
    28321 acaagaaatg acgcggcgtg gcgttgccgt tatagacgcg gctggcgatt tccagcgccg
    28381 cccggtcagg gcgcagggtg aaacctgtca tcacctgtaa accatgcatc ggctccgttt
    28441 cgccgaccca caccgtctgt gcgccgtctt catgggcttc gagggtgaaa tcaacgggca
    28501 taaaggtggt cgggcgatgg tgttgcggcc agttaaactc aatcccaccg gagatccacg
    28561 gtcccagcag ccccaccagc gcaggtttaa tgacttcatt gtgataaaca aaatcgcgtt
    28621 gtttcacttt atcccatgcg cgatgcacac gaccgcccag ttccggcagg atcatcactt
    28681 tgatgtagtc gttttccagc cacaccgcct gccaggattt cagggttttc tgctcgctca
    28741 gcgtatcggt cacgccatag ggataaaccg cgccggacga tccctgataa acgcgatttt
    28801 ccaggaacat gggatgtata tcctgcggcc cggtttcata ggtcgggatc tcaacgcgct
    28861 cttgccacac ttttactgga gtcatggtgc cctcaatatt aacaagacat actgaattaa
    28921 aagatttgtg gcagtgtatt gaacaatctg gcaatgtttt cgcggaataa tcacgcaatt
    28981 aactaaacaa ggtttagtga agatgagagc ctgcattaac aatcaacaga tccgccacca
    29041 taacaaatgc gtgattctgg aactgctgta ccggcaaaag cgcgccaata aatcaacgct
    29101 ggcccgactg gcgcaaattt cgattccggc agtcagtaat attttgcagg aactggaaag
    29161 cgaaaaacgg gtggtgaata tcgacgatga aagccatacg cgcgggcata gtagcggtac
    29221 atggctgatt gcgccggaag gtgactggac gctgtgcctg aacgtgacgc ccaccagtat
    29281 tgagtgtcag gttgctaatg cttgtttaag tccgaaaggt gaatttgagt atttacagat
    29341 tgatgcaccg acgccgcagg cgctgctgtc cgaaatcgaa aaatgctggc atcgccaccg
    29401 taaattgtgg ccggaccata ccatcaacct ggcgttggca atccacggtc aggttgatcc
    29461 tgtgaccggc gtgtcgcaaa ccatgccgca agcgccgtgg acaacgccgg tggaggtgaa
    29521 atatctgctg gaagagaagc tcggcattcg ggtgatggtc gataatgact gcgtgatgct
    29581 ggcgctggcg gagaaatggc aaaataattc gcaggaacgg gatttctgcg tgatcaacgt
    29641 tgattacggc attggctcgt cgttcgtgat taacgagcaa atttatcgcg gcagtttgta
    29701 tggtagcgga cagattggtc acaccatcgt taatccggat ggcgtcgtct gcgactgtgg
    29761 acgttatggc tgcctggaaa ccgtcgcctc gttaagcgca ttaaaaaaac aggcgcgggt
    29821 atggctaaaa tcacaaccgg ttagtactca acttgatcct gaaaaactga ctacagcgca
    29881 gttaatcgct gcctggcaaa gtggagaacc gtggatcacc agctgggttg atcgctctgc
    29941 caatgccatt ggtttgagtc tgtataactt cctcaacatc ctcaatatta atcagatttg
    30001 gttgtacggt cgcagttgtg cctttggtga gaactggctt aatactatta ttcgccagac
    30061 aggatttaac ccgttcgacc gcgacgaagg accgagcgtg aaagcgacgc aaattggctt
    30121 tgggcaatta agccgcgcac aacaggtgct gggaattggc tatttgtatg ttgaggcgca
    30181 gttacgacag atttgatggc gcgataacgt agaaaggctt cccgaaggaa gccttgatga
    30241 tcataaacga aaaattgcct gatgcgctac gcttatcagg cctacacgga gattgcaata
    30301 tattgaattt gcaaagtttt gtaggccgga taaggcgttc acgccgcatc cggcatgaac
    30361 aacgagcaca ttgacagcaa atcaccgttt cgcttatgcg taaaccgggt aacgtgcgca
    30421 gatgtcgaga actttacctt tgatgcgctc gataacggct tcatcattga tgctgtccag
    30481 cacgtcacac atccagccag ccagttcttt cgcttcggct tctttaaagc cgcgacgggt
    30541 aatcgccgga gtacctacac gaataccgga ggtcacaaac gggctcttcg gatcgttcgg
    30601 tacgctgttt ttgttgacgg tgatgttagc acggcccaga gcggcgtctg cttctttacc
    30661 ggtcaggttt ttatcaacca gatcaaccag gaacaggtgg ttatcagtgc cgccggaaac
    30721 cactttgtag ccgcgctcga ggaacacttc taccatcgct ttagcgtttt tagcgacctg
    30781 ctgctggtaa gttttgaact caggctccat cgcttctttc agagcaaccg ctttaccggc
    30841 gattacgtgc atcaacggac cgccctgacc accagggaaa acggcagagt tcagtttttt
    30901 gtacagctct tcgctaccac ctttcgccag gatcaggccg ccgcgcggac ccgccagggt
    30961 tttgtgagtg gtggtagtaa caacgtgagc atgaggaacc gggttcgggt agacgccagc
    31021 agcaaccagg cccgcaacgt gcgccatatc aacgaacagg taagcaccga tgctgtcagc
    31081 gatttcacgc attttcgccc agtccaccac gccggaatat gcagagaaac caccgataat
    31141 cattttcggc ttgtgttctt tggcttgttt ttccagatcg gcgtagtcga tatgaccggt
    31201 agcatcgata ccgtaaggaa cgatgttgta cagtttaccg gagaagttaa ccggagaacc
    31261 gtgagtcagg tgaccgccat gcgccaggtt catacccaga acggtatcac ctggttccag
    31321 cagcgcggtg tagaccgcaa agttagcctg ggagccggag tgcggctgga cgttagcgta
    31381 gtcagcgccg aacagttctt tcgcacgatc gatcgccagt tgttcaacga tatcaacata
    31441 ctcgcaaccg ccgtagtagc gtttgcccgg ataaccttca gcatatttgt tggtcagctg
    31501 agaaccctgc gcctgcatta cgcgcgggct ggtgtagttt tcggaggcga tcagttcgat
    31561 gtgctcttcc tgacgtactt tttcctgctc catagcctgc cacagttcgg catcataatc
    31621 ggcaatgttc atttcacgct ttaacatccg catctcctga ctcagctaac aataaaattt
    31681 ttggccttta taggcggtcc tgttggacaa cggcgaacag tataaccgaa tcattgtgcg
    31741 ataacaggtc ttgacaaagg aatttacgca aacgattacc ttcaggctac gcaaggcttt
    31801 ggagaataaa gagcttgcaa ccggaaacgg atttcttttc aggtttgtga tgcaaatttt
    31861 tcacttcatc acattctttc tgaaaaacac caaagaacca tttacattgc agggctattt
    31921 tttataagat gcatttgaga tacatcaatt aagatgcaaa aaaaggaaga ccatatgctt
    31981 gacgctcaaa ccatcgctac agtaaaagcc accatccctt tactggtgga aacggggcca
    32041 aagttaaccg cccatttcta cgaccgtatg tttactcata acccagaact caaagaaatt
    32101 tttaacatga gtaaccagcg taatggcgat caacgtgaag ccctgtttaa cgctattgcc
    32161 gcctacgcca gtaatattga aaacctgcct gcgctgctgc cagcggtaga aaaaatcgcg
    32221 cagaagcaca ccagcttcca gatcaaaccg gaacagtaca acatcgtcgg tgaacacctg
    32281 ttggcaacgc tggacgaaat gttcagcccg gggcaggaag tgctggacgc gtggggtaaa
    32341 gcctatggtg tactggctaa tgtatttatc aatcgcgagg cggaaatcta taacgaaaac
    32401 gccagcaaag ccggtggttg ggaaggtact cgcgatttcc gcattgtggc taaaacaccg
    32461 cgcagcgcgc ttatcaccag cttcgaactg gagccggtcg acggtggcgc agtggcagaa
    32521 taccgtccgg ggcaatatct cggcgtctgg ctgaagccgg aaggtttccc gcatcaggaa
    32581 attcgtcagt actctttgac tcgcaaaccg gatggcaaag gctatcgtat tgcggtgaaa
    32641 cgcgaagagg gtgggcaggt atccaactgg ttgcacaatc acgccaatgt tggcgatgtc
    32701 gtgaaactgg tcgctccggc aggtgatttc tttatggctg tcgcagatga cacaccagtg
    32761 acgttaatct ctgccggtgt tggtcaaacg ccaatgctgg caatgctcga cacgctggca
    32821 aaagcaggcc acacagcaca agtgaactgg ttccatgcgg cagaaaatgg cgatgttcac
    32881 gcctttgccg atgaagttaa ggaactgggg cagtcactgc cgcgctttac cgcgcacacc
    32941 tggtatcgtc agccgagcga agccgatcgc gctaaaggtc agtttgatag cgaaggtctg
    33001 atggatttga gcaaactgga aggtgcgttc agcgatccga caatgcagtt ctatctctgc
    33061 ggcccggttg gcttcatgca gtttaccgcg aaacagttag tggatctggg cgtgaagcag
    33121 gaaaacattc attacgaatg ctttggcccg cataaggtgc tgtaatttga tgttgccgga
    33181 tggaaacatc cggcaaccct tgacgcggtt taaattgccg cgtcgtcctc ttcaccggta
    33241 cggatgcgaa tgacccgtgc cacgtcaaag acgaagattt taccgtcacc gattttgccg
    33301 gtttgcgccg tgcgaataat ggtatcgaca caggtatcga caatgtcgtc cggtacgaca
    33361 atctcaattt tcactttcgg cagaaaatcc accatatact ccgcgccgcg gtacagctcg
    33421 gtatggcctt tctggcgacc aaagcctttc acttcggtca ccgtcatgcc ggtaataccg
    33481 acttcggcca gtgcttcgcg gacatcgtcc agcttgaagg gttttataat cgcatcaatc
    33541 tttttcatgc tattccttga aaaggtcgcc tgtcttttga tctgctaaac gtaacacata
    33601 acgccaattc attccttgaa atcgtttgca tccagctcgt gtcgggaaag cagtttataa
    33661 aattctgtcc ggttgcgccc cgccattctc gccgcgtggg tgacgttgcc tttggtaatt
    33721 tgcagcagct tacgcaaata gttgagttca aactgattac gtgcctcaac aaaggttggc
    33781 agcgccgtgt tttcaccctc cagcgcctgc tctactaatg catcgctaat aaccggagat
    33841 gaggtcagcg ccacgcactg ttcaatcacg ttgaccaact ggcgcacatt acccggccag
    33901 ctagcggtca tcaggcgttt catcgcatca gtggagaacg cgcggacaaa cggtttatgt
    33961 cgctctgccg cctggcgcaa caggtgattt gccagaagcg gaatgtcttc tgtgcgctct
    34021 gccagagccg gaattttcag gctgacaacg ttgaggcggt aatagagatc ttcgcggaat
    34081 tccccgcgcg ccatcgcttt tggcagatca cggtgagtgg cagaaataat ccgcacattg
    34141 atatcaatat cgcggttact gcccagcggg cgcactttac gctcctgcaa cacgcgcagc
    34201 agtttgacct gtaacggcgc aggcatatcg ccaatttcat cgagaaatag cgttccgcct
    34261 tccgccgcct ggaataaacc ttcgcgattg ctgacagcgc cagtaaacgc gccacgcgca
    34321 tgaccaaaca gctccgactc cagcaattgc tcgggcaatg cgccacagtt aatagcaata
    34381 aatggtttgc tgttgcgcgg gctggcgttg tggatagcct gggcgaaaat ctctttcccg
    34441 gtgccgctct gaccgttaat caaaacgctg acgtctgatt gcgccaccag ccgcgcctgt
    34501 tccagcaaac gcagcatcag cgggctgcgg gtgacaattg cctcgcgcca gcgttcatcg
    34561 gtggctggcg cggattgctc cagcgcatcg tcaattgcct gatatagcgc gtctttgtcg
    34621 acaggcttgg tgaggaaact aaaaacgccc tgctgtgttg cagcaacggc atcggggata
    34681 gaaccatgcg cagtaagaat aattactggc attcccggct gcactttctg gatttcagca
    34741 aatagctgca taccgtccat ttcatccatc cgcaggtcgc tgataactaa atctactttt
    34801 tcgcgattca gtacccgtaa tccttcagcg ccactttccg ccgtgaccac actgtagcct
    34861 tcgctggtca ggcgcaggcc aagcagtttc agcaatcccg gatcgtcatc gaccaataat
    34921 aaatgcgcag gtttatggct catcaggagt gacctcatgg gtggatggcg cgggcttttc
    34981 actctcgtgc ggcgtatccg gcgagaaatt tccagccggt ttgcgggtcg agagctggcg
    35041 ttcaatatcg gtcaggtttt ccagcttgcg ggtggtgagt tccagttgct gctgtagaac
    35101 gtgatgttgc tggcgcaatg tatccagctc gctgtcgctg gactgctgga gtttactgta
    35161 acgttggcgc tcttccgcca gttgcagttg cagcgcctga ccgtcgcgcc agagttgata
    35221 cagtgggcga acctgtgccg ggatctcggt acttaacgct tcaatacgcg cgaccagctg
    35281 gcggcgctca tacggcgtaa ttttggcgtc ggcgagcaaa atcccttgtt taaaggtatt
    35341 ttgccagctg ccgtcgtcat attggcgagc ttgctgacgc gactgcgcag gcattaaacg
    35401 atcagcacaa tccatcgccc gcagccagta aagcggattg gtttcggttg atttaccttg
    35461 cagcgcccag atgtcgctac attcagtaga acgatagtca gccagttgat aaaccggaat
    35521 tttttcttct gctggcgtat caatggctgg cttattgtga ttctgcacgc aacccagcaa
    35581 tgccagacat ggcagccctg ccagccacag ccgtcggggc aataatcgtt gaaaaatgtg
    35641 tcgcatattc accagactta aagcctatcc cagtgggcgt aattgttgca gacagtctgg
    35701 acatggacag cgcggagaaa ccggagcgta catatcgtac gtgaagattt cgagcactgc
    35761 ccggggccga actgacaaat aaaatagcct gataggatag gctgttagca ttatttcgtg
    35821 tttttcgacg acggtaattc aatgcggaaa caaacgtctt gcccgctctc gtcgaccaga
    35881 tacagttccc cttgcatacg gcgaatacaa tccctggcaa tgcttaatcc cagaccgctg
    35941 cccttcaccg ccccttttcg ctggtggctt ccctgaaaaa agggttcgaa gatcatggcg
    36001 cgttcctctt gcggaatggg cgtgcctgta ttgatgacat caatataaac ccgcgcacca
    36061 tgtaaactgc tgcgaaggca aatgttaccg gattcagccc cgtagtgcac cgcattggag
    36121 taaagattat ccagtacgct catcagcagc attggctccg ccaggcaagc tgttgctttg
    36181 agatcgacgt cggtatgcat cattttagcc cgtgcgggca ggctatgagc agaaaccact
    36241 gtctccacca gcggtgctaa ctcaacattc tccagttcca ccgcactgtc cgcctgttta
    36301 cggttgtaat caagcagttg ttcgatcagt ttttgcaaat tgcggctgct gctatcaaga
    36361 atgctcacca cctctttttg ctctggcgta agcggcccga caacctggtc agccagtaat
    36421 tcagtgccct cgcgcatact cgccagtggc gtttttaatt catgagataa atgtcttaaa
    36481 aattgatggc gttgggattc cagccatgac aggcgctcac ttaaccaaag aatacgttgc
    36541 ccaaccgagc gtaactcgct cggtccactg aacgagacgc tattgcccag agaacgccct
    36601 tcccccagac ggttgatcat gcgctcgata tttttcaccg gcccgataat catccgcgtg
    36661 aaaagcagta ccattaccag actcaccaga aatagcacca gcgattgcca accaaaatat
    36721 tgcccacgtt cggcgatttc acgctgaagc tgctgcccac gagagaacac cactgtgcgc
    36781 gtggcctgta ccatttcggt attggcactg gcaaaggctt caagacgtgc ggcggcggca
    36841 gcatcgggac cgctgttgtt acactgaagt tgagccagat tgtgcaaatc ctgacgtaat
    36901 gcctggtaga gtttatcgtc cggcagcacg cctgcgtggg catcgagcat ttcgctgtaa
    36961 cgcttgcgct ggctttgata aaccttcgcc agggttgggt cgtccagcac gcaatactga
    37021 cggtaactac gctccatctc cagcgccgcg ttggtcatcg cttcactgcg ccgggcatca
    37081 ataagcgtag tgcggttaac cagcgccgcc tgatcgctaa gcgcattcag gctttgccag
    37141 gcttgccatg ccagcaccaa caggggcagc agaatcagca aaaatgccag cattaccagt
    37201 tgtcgtaatg agcggggaaa aacgggccag cgtttcaagg tgttactctc gtcagacgcg
    37261 aatagcctga tgctaaccga ggggaagttc agatacaaca aagccgggaa ttacccggct
    37321 ttgttatgga ataaggcggt gcctaactcg acgtttcgcc cgatggttga tatagctacg
    37381 ctgatatcag aagttggacg gcaggcacct tgttgtgcgt cattcgtatt ttatgtagca
    37441 cgtcccgaag gggctgacat aagtcggtga atgagccact ggttactatt atgcagtaac
    37501 tgtgccaata aagaaaatag tttggtaacg tactgattat acgttgcttt gaggggttta
    37561 tgtcttctcc gctgtttgaa tgtaaatcag ccactgtgtc gct
//
LOCUS       DAPHBJ010000022        36294 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.022, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000022 DAPHBJ010000000
VERSION     DAPHBJ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.022"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            208..1581
                     /gene="aroP"
                     /locus_tag="RHZ76_001030"
     CDS             208..1581
                     /gene="aroP"
                     /locus_tag="RHZ76_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HDV2811707.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1624..2478)
                     /gene="ampE"
                     /locus_tag="RHZ76_001031"
     CDS             complement(1624..2478)
                     /gene="ampE"
                     /locus_tag="RHZ76_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HDV2811708.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2475..3026)
                     /gene="ampD"
                     /locus_tag="RHZ76_001032"
     CDS             complement(2475..3026)
                     /gene="ampD"
                     /locus_tag="RHZ76_001032"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HDV2811709.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            3114..4007
                     /gene="nadC"
                     /locus_tag="RHZ76_001033"
     CDS             3114..4007
                     /gene="nadC"
                     /locus_tag="RHZ76_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDV2811710.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4210..4650
                     /gene="ppdD"
                     /locus_tag="RHZ76_001034"
     CDS             4210..4650
                     /gene="ppdD"
                     /locus_tag="RHZ76_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HDV2811711.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQNDSALQQACEDVFRFDDAN"
     gene            4660..6043
                     /gene="gspE"
                     /locus_tag="RHZ76_001035"
                     /pseudo
     CDS             4660..6043
                     /gene="gspE"
                     /locus_tag="RHZ76_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            6033..7235
                     /gene="hofC"
                     /locus_tag="RHZ76_001036"
     CDS             6033..7235
                     /gene="hofC"
                     /locus_tag="RHZ76_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HDV2811712.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLRMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNIAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7270..8313)
                     /gene="guaC"
                     /locus_tag="RHZ76_001037"
     CDS             complement(7270..8313)
                     /gene="guaC"
                     /locus_tag="RHZ76_001037"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDV2811713.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8469..8513)
                     /locus_tag="RHZ76_001038"
     CDS             complement(8469..8513)
                     /locus_tag="RHZ76_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2811714.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8538..9158
                     /gene="coaE"
                     /locus_tag="RHZ76_001039"
     CDS             8538..9158
                     /gene="coaE"
                     /locus_tag="RHZ76_001039"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDV2811715.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9158..9901
                     /gene="zapD"
                     /locus_tag="RHZ76_001040"
     CDS             9158..9901
                     /gene="zapD"
                     /locus_tag="RHZ76_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HDV2811716.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     HNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9911..10108
                     /gene="yacG"
                     /locus_tag="RHZ76_001041"
     CDS             9911..10108
                     /gene="yacG"
                     /locus_tag="RHZ76_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HDV2811717.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10315..10713)
                     /gene="mutT"
                     /locus_tag="RHZ76_001042"
     CDS             complement(10315..10713)
                     /gene="mutT"
                     /locus_tag="RHZ76_001042"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HDV2811718.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVERWEGEPWGKEGQPG
                     EWMSLVGLNADEFPPANEQVIAKLKRVYVR"
     gene            complement(10773..13478)
                     /gene="secA"
                     /locus_tag="RHZ76_001043"
     CDS             complement(10773..13478)
                     /gene="secA"
                     /locus_tag="RHZ76_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDV2811719.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13540..14052)
                     /gene="secM"
                     /locus_tag="RHZ76_001044"
     CDS             complement(13540..14052)
                     /gene="secM"
                     /locus_tag="RHZ76_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HDV2811720.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(14283..15200)
                     /gene="lpxC"
                     /locus_tag="RHZ76_001045"
     CDS             complement(14283..15200)
                     /gene="lpxC"
                     /locus_tag="RHZ76_001045"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HDV2811721.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEVWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15301..16452)
                     /gene="ftsZ"
                     /locus_tag="RHZ76_001046"
     CDS             complement(15301..16452)
                     /gene="ftsZ"
                     /locus_tag="RHZ76_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDV2811722.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16513..17775)
                     /gene="ftsA"
                     /locus_tag="RHZ76_001047"
     CDS             complement(16513..17775)
                     /gene="ftsA"
                     /locus_tag="RHZ76_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDV2811723.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17772..18602)
                     /gene="ftsQ"
                     /locus_tag="RHZ76_001048"
     CDS             complement(17772..18602)
                     /gene="ftsQ"
                     /locus_tag="RHZ76_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HDV2811724.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPERTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18604..19524)
                     /gene="ddlB"
                     /locus_tag="RHZ76_001049"
     CDS             complement(18604..19524)
                     /gene="ddlB"
                     /locus_tag="RHZ76_001049"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDV2811725.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19517..20992)
                     /gene="murC"
                     /locus_tag="RHZ76_001050"
     CDS             complement(19517..20992)
                     /gene="murC"
                     /locus_tag="RHZ76_001050"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDV2811726.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARGGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPARVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21046..22113)
                     /gene="murG"
                     /locus_tag="RHZ76_001051"
     CDS             complement(21046..22113)
                     /gene="murG"
                     /locus_tag="RHZ76_001051"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDV2811727.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRVARA"
     gene            complement(22110..23354)
                     /gene="ftsW"
                     /locus_tag="RHZ76_001052"
     CDS             complement(22110..23354)
                     /gene="ftsW"
                     /locus_tag="RHZ76_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HDV2811728.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23354..24670)
                     /gene="murD"
                     /locus_tag="RHZ76_001053"
     CDS             complement(23354..24670)
                     /gene="murD"
                     /locus_tag="RHZ76_001053"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDV2811729.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24673..25755)
                     /gene="mraY"
                     /locus_tag="RHZ76_001054"
     CDS             complement(24673..25755)
                     /gene="mraY"
                     /locus_tag="RHZ76_001054"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDV2811730.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25749..27107)
                     /gene="murF"
                     /locus_tag="RHZ76_001055"
     CDS             complement(25749..27107)
                     /gene="murF"
                     /locus_tag="RHZ76_001055"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDV2811731.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27104..28591)
                     /gene="murE"
                     /locus_tag="RHZ76_001056"
     CDS             complement(27104..28591)
                     /gene="murE"
                     /locus_tag="RHZ76_001056"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDV2811732.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28578..30344)
                     /gene="ftsI"
                     /locus_tag="RHZ76_001057"
     CDS             complement(28578..30344)
                     /gene="ftsI"
                     /locus_tag="RHZ76_001057"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HDV2811733.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30360..30725)
                     /gene="ftsL"
                     /locus_tag="RHZ76_001058"
     CDS             complement(30360..30725)
                     /gene="ftsL"
                     /locus_tag="RHZ76_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDV2811734.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30722..31663)
                     /gene="rsmH"
                     /locus_tag="RHZ76_001059"
     CDS             complement(30722..31663)
                     /gene="rsmH"
                     /locus_tag="RHZ76_001059"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDV2811735.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31665..32123)
                     /gene="mraZ"
                     /locus_tag="RHZ76_001060"
     CDS             complement(31665..32123)
                     /gene="mraZ"
                     /locus_tag="RHZ76_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDV2811736.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIYHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32725..33729)
                     /gene="cra"
                     /locus_tag="RHZ76_001061"
     CDS             complement(32725..33729)
                     /gene="cra"
                     /locus_tag="RHZ76_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HDV2811737.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33811..33897)
                     /locus_tag="RHZ76_001062"
     CDS             complement(33811..33897)
                     /locus_tag="RHZ76_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HDV2811738.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33909..34400)
                     /gene="ilvN"
                     /locus_tag="RHZ76_001063"
     CDS             complement(33909..34400)
                     /gene="ilvN"
                     /locus_tag="RHZ76_001063"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDV2811739.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSGKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34403..36127)
                     /gene="ilvI"
                     /locus_tag="RHZ76_001064"
     CDS             complement(34403..36127)
                     /gene="ilvI"
                     /locus_tag="RHZ76_001064"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HDV2811740.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVCSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            36246..36290
                     /locus_tag="RHZ76_001065"
     CDS             36246..36290
                     /locus_tag="RHZ76_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2811741.1"
                     /translation="MKRNDKILHYRGLN"
BASE COUNT         8672 a        10193 c         8911 g         8518 t
ORIGIN      
        1 aaacggtttc tttttaagca actcatcttc aaccatgcat aaagcgggtg cattcgctgc
       61 cgcataccat tattcttgat ctgacggaag tctttttgta acaattcaaa cttctttgat
      121 gtaaacaaat taatacaata aacggaattg caaacttaca cacgcatcac tgcgtagatc
      181 aaaaaaacaa ccaccgcacg aggtttcatg atggaaggtc aacagcacgg cgagcagcta
      241 aagcgcggcc ttaaaaaccg ccatattcag cttatcgcgc tgggtggcgc gatagggacc
      301 gggttattcc tgggtagcgc ctccgtaata cagtccgcag ggccagggat tatcctgggt
      361 tacgccattg ctggttttat cgcctttctg atcatgcgtc agctgggtga aatggtggtc
      421 gaagaacctg tcgcaggctc ctttagccac tttgcttata aatactgggg cagttttgcc
      481 ggtttcgcct ctggctggaa ctactgggta ctgtacgttt tagttgccat ggcagagctg
      541 actgccgtgg gtaaatacat tcagttctgg tatccggaaa tcccaacctg ggtttctgcc
      601 gccgtattct ttgtggtgat taacgccatc aacctgacca acgttaaagt gtttggcgag
      661 atggagttct ggtttgccat tatcaaagtt attgcggtag tagcgatgat catcttcggc
      721 ggctggctgc tgttcagtgg caacggcggc ccgcaggcga gcgttagcaa cctgtgggat
      781 cagggtggtt tcctgccgca cggcttcacc ggactggtga tgatgatggc gattatcatg
      841 ttctcgttcg gtggtctgga actggtgggg atcaccgcag cagaagctga taacccggag
      901 caaagtatac cgaaagcaac taaccaggtt atctaccgca tcctgatttt ctatattggt
      961 tcgttagccg ttctgctctc actgatgccg tggacccgcg ttaccgccga taccagtccg
     1021 tttgtgctga tcttccacga gttaggcgat acctttgtgg cgaatgcgct gaacatcgtg
     1081 gtactgactg cggcgctctc cgtgtacaac agctgcgtat attgcaacag ccgtatgctg
     1141 tttggtctgg cacaacaggg taatgcgcca aaagcgctgg cgtctgtcga taaacgtggt
     1201 gtaccagtaa ataccattct ggtgtctgca ctggtaacgg cgttgtgcgt actgattaac
     1261 taccttgccc cagagtccgc tttcggactg ttaatggcgc tggtggtatc tgcactggta
     1321 atcaactggg cgatgattag cctggcgcat atgaaattcc gtcgcgccaa gcaggaacaa
     1381 ggcgtggtaa ctcgcttccc tgctctgctt tatccgctgg gtaactggat ctgcctgctg
     1441 tttatggcgg cggtactggt gattatgctg atgaccccag gaatggcgat ttcggtatac
     1501 ctgatcccgg tatggctgat cgtgttaggt atcggctatc tgtttaaaga gaaaaccgcc
     1561 aaagccgtaa aagcgcatta atctctctac gccctcaccc gtacagggtg agggcaataa
     1621 tctttacacc aacgccccgt aaatcgtcag tagtgcaatc accaccacga ccacgaacga
     1681 ggttttcttc gccattgaaa ccgctgcctt cggcgtctcc accttatcga catgcggttc
     1741 acgcgccaga gagaactgcg ccagacgcgt taacacctga tactgcgaag tatggaaatc
     1801 acccagcgaa gcaaaccagg ccggtaacgc tttctcacca tgaccgatca aggcatatac
     1861 cacacccgca agacgaaccg gcacccaatc cagtacatga agcacggcat caatgccgga
     1921 ctgtaaacga tgatgcggcg tctgatatcg cgccagccag tattgccatg cacgcaaaaa
     1981 cgcatacccc atcagcgtaa cgggtcccca ggttcccccc acaatcagcc agaacagcgg
     2041 tgcaagataa aaacgaaagt taatccacag caatgcattt tgcagctcac gcaaatactc
     2101 acgttcgtcg cagcctgccg ggacgccgtg aatcatggtg agttcgccag ccatcgtggc
     2161 acgggcatgg ctatcattac gtgaagcagc tgtcagataa gcatgataat gaagacgaac
     2221 tttacctgcg ccaatacaca gcaaaccaat cagcagccac accagtagcg tgggaacgtt
     2281 gaacaatact ccctgcaatg cgcgtaacag taaaaacgtc acgcccatcg caataatggt
     2341 catgcctaac gtgcgcccga gagaaaaatg tttcacccgc cgaaagaacg cttcaagacg
     2401 atgatcaagc tgccagtgct cgcccaactt aaacaggcgc tcgaaaatta acaccagtaa
     2461 ggttgtaaat agcgtcatgt tgtctccttg ctgaccagca cacgaaaccg tgcccaatca
     2521 aatgcaggac cgggatcggt tttccgatcc ggcgcaatat cacaatggcc cgtcatgttt
     2581 ttagcgatat ccggatagca atcaatcagt gcccgcgtaa ccgccgcaag ctgttgatac
     2641 tgcgcatcgg tatacgccag cgtatcggtg ccttcaagct caatcccaat agaaaaatca
     2701 ttgcagcgtt cgcgcccctg atactgagag actcccgcat gccatgcacg tttatcgaaa
     2761 ggaacatact ggactatttc accatcacgg cgaatcaaac agtgagcgga gacgcgcaaa
     2821 tgggcgatct cagcaaagaa aggatgtgcc tgcggatcaa tagttccagt gaataatgcg
     2881 tcgatccacg gaccgccaaa ctcgcctggc ggcaggctaa tattgtgcac caccagcagg
     2941 gtgggtgttt cgtcatccgg gcggcaatcg taatgtggtg agggaacgcg gcgcgcgcca
     3001 accagccacc cctgttctaa caacatgcag gatctcctta tatgtggtgc taatacccgg
     3061 ttcagagtag catgtttcta ccttatgatt cgttagctat ctggagtttt aacatgccgc
     3121 ctcgccgcta taaccctgac acccgacgtg acgagctgct ggaacgcatt aatctcgata
     3181 tccccggcgc ggtggcccag gcgctgcggg aagatttagg cggaacagtc gatgccaaca
     3241 atgatattac ggcaaaactt ttaccggaaa attctcgctc tcatgccacg gtgatcaccc
     3301 gcgagaatgg cgtcttttgc ggcaaacgct gggttgaaga ggtgtttatt caactggcag
     3361 gcgacgatgt caccataatc tggcatgtgg atgacggcga tgtcatcaat gccaatcaat
     3421 ccttgttcga acttgaaggc ccatcccgcg tgctgttaac gggcgaacgc actgcgctta
     3481 attttgtgca aaccctttca ggagttgcca gtaaggtacg ccactatgtc gaattgctgg
     3541 aaggcaccaa cacgcagttg ttggatacgc gcaaaacctt acccggcctg cgttcagctc
     3601 tgaaatacgc ggtactttgc ggcggcggag cgaatcaccg tctggggctt tctgatgcct
     3661 tcctgatcaa agaaaaccat attattgcct ccggctcagt gcgccaggcg gtcgaaaaag
     3721 cgtcctggct gcacccggat gcgccagtag aagtcgaagt agagaatctg gaagaacttg
     3781 atgaagccct gaaagcagga gccgatatca tcatgctgga taacttcgaa acagaacaga
     3841 tgcgcgaagc cgtcaaacgc accaacggca aggcgctact ggaagtgtct ggcaacgtca
     3901 ctgacaaaac actgcgtgaa tttgccgaaa cgggcgtgga ctttatctcc gtcggtgcgc
     3961 taactaaaca cgtacaagca ctcgaccttt caatgcgttt tcgctaatag ttgacagatt
     4021 tatccgctcc atcgcgggcg gataatctcc ccttccccaa ctttcttcgt aacgcctcgc
     4081 aaatttcttg tctttcgctg caacgaaata aaaaacctcg gaatctgctt tcccgtttcg
     4141 ttttttgccc ctcgcctgtg ctgatggcgt ttgccaaagt agcgccaacc aaatcaagga
     4201 gcgaaacaga tggacaagca acgcggtttt acacttatcg aactgatggt ggttattggc
     4261 atcattgcca ttttaagcgc cattggtatt cccgcttatc aaaactacct gcgcaaagct
     4321 gcactcaccg acatgctaca aacctttgtg ccttaccgta ccgccgtaga gttgtgcgcg
     4381 ctggaacatg gtggattaga tacctgcgac ggtggcagca atggcattcc ctcgcctacc
     4441 accacccgct atgtttcagc catgagtgtg gcaaaaggcg tggtgtcgct gaccgggcaa
     4501 gaaagtctca atgggctaag cgtcgtcatg acgccgggtt gggataacgc aaacggcgtc
     4561 accggctgga cgcgcaactg caatattcaa aatgacagcg cattgcagca agcctgtgaa
     4621 gatgtcttcc gctttgatga cgccaactaa ggagcggcaa tgaatattcc acagctcacg
     4681 gccctgtgcc tgcgttatca gggagtcttg ctggatgcca gcgaagaagt ggttcatgtt
     4741 gcggtggtcg atgccccctc acatgagttg ctggacgcat tgcatttcgc taccaccaaa
     4801 cgtattgaga tcacctgctg gacgcgccaa caaatggaag gtcacgccag tcgcacacaa
     4861 cagacattgc ccgtagctgt tcaggagaag catcagccca aagcagagtt gctgactcga
     4921 acgttacaat ctgcgctgga acaacgcgcg tctgatattc atatcgaacc agcggacaat
     4981 gcctaccgca tccgcttgcg tatcgacggc gtattgcatc ctttaccgga tgtttcaccg
     5041 gatgccggag tcgcattaac cgccagatta aaagtgctgg gaaacctgga tattgcggaa
     5101 catcgcctgc cgcaggacgg gcaattcact gtcgaactgg caggaaacgc cgtctcattt
     5161 cgtattgcga ccttaccatg tcggggtggt gaaaaggtgg tattaaggtt gttacagcag
     5221 gtgagccagg cactggatgt taacacgctg ggaatgcagc cgttacaact ggcggacttt
     5281 gctcatgcct tgcaacaacc acagggactg gtgctggtaa ctggccctac cggcagcggc
     5341 aaaacggtca cgctttatag tgccctgcaa acgctgaata ccgctgacat taatatttgt
     5401 agcgtcgaag atccggttga gatccccata gccggactaa accagacgca aatccatccg
     5461 cgtgccgggc tcacctttca gggcgttttg cgtgcgttat tgcgccagga tcctgacgtc
     5521 atcatgatcg gagagatccg cgatggtgaa acggcagaaa ttgccattaa agccgcgcaa
     5581 accggtcacc tggtgttgtc cacgctacac accaattcca ccactgaaac gctggtacgt
     5641 ttacagcaaa tgggggtcgc ccgctggatg ctatcatcgg cgcttacgct ggtaatagcc
     5701 cagcgtctgg tacgtaaact ttgcccacat tgtcgccgac agcaagggga gctcatccat
     5761 attccagaca ctgtatggcc gtcgccgctg cccccactgg caggcacccg gtgtacattg
     5821 ctaccacggt ttttatggtc gtacggcctt atttgaagtt ctgcccataa cgccggtcat
     5881 tcgtcagctt atttccgcta ataccgacgt tgaatcgctg gaaacgcacg cacgacaggc
     5941 gggtatgcgt acgctttttg aaaacggctg cctggccgta gagcaaggct taaccacctt
     6001 tgaagagtta atccgcgtac tggggatgcc gcatggcgag taagcaactc tggcgctggc
     6061 atggcataac cggcgacggc aatgcgcaag atgggatgct gtgggcagag agccgtactt
     6121 tactgcttat ggcactacag caacagatgg ttaccccact aagcctgaag cgaatcgcca
     6181 tcaattctgc gcagtggcga ggagataaaa gcgcggaagt cattcatcaa ctggcgacgc
     6241 tactcaaagc cgggttaacg ctttctgaag ggctggcact gctggcggaa cagcatccca
     6301 gtaagcaatg gcaagcgttg ctgcaatctc tggcacacga tctcgaacag ggcattgcct
     6361 tttccaatgc cttattaccc tggtcagagg tatttccgcc gctctatcag gcgatgatcc
     6421 gcacgggtga actgaccggt aagctggatg aatgctgctt tgaactggcg cgtcagcaaa
     6481 aagcccagcg tcagttgacc gacaaagtga aatcagcgtt gcgttatccc atcatcattt
     6541 tagcgatggc aatcatggtg gttgtggcaa tgctgcattt tgttctgccg gagtttgccg
     6601 ctatatataa gaccttcaac accccactac cggcactaac gcaggggatc atgacgctgg
     6661 cagactttag tggcgaatgg agctggctgc tggtgttgtt cggctttctg ctggcgatag
     6721 ccaataagtt gcggatgcgc cgcccgacct ggcttatcgc gcggcagaaa ttgctgttac
     6781 gcatcccgat tatgggttca ctgatgcggg gacaaaaact cacgcagatc tttacgattc
     6841 tggcgctgac tcaaagtgca ggcattactt ttttacaggg cgtagagagc gtcagagaaa
     6901 caatgcgctg cccgtactgg gtgcaacttc tgacacaaat ccagcacgat atcagtaacg
     6961 gtcatcccat ctggctggcg ttaaaaaatg ccggtgagtt tagcccgctc tgtttgcaat
     7021 tagtgagaac gggagaggcc tccggctcgc tggatctcat gttagacaac atcgcccatc
     7081 atcatcggga taacacaatg gcgctggcgg ataacctagc agccttactg gaaccggcgt
     7141 tgctgatcat aacgggagga attatcggta cgctggtggt ggcgatgtat ctgccaattt
     7201 tccatttagg cgatgcgatg agtgggatgg gataacgctg gcgtgttgct ccacaccagc
     7261 gttggaagat tacaggttgt tgaagatgcg gttttcttgt tcctgcacac ggataaatgt
     7321 ggtgcgcttg gtcagttctt tcaggcgtga agccccaacg tatgtacaag ctgaacgcag
     7381 gccgcccaaa atatcacgcg cggtattttc aaccgggcct cgcagcggca gcttaacggt
     7441 tttaccttct gctgcgcgat attcagcaac gccgccaacg tgacgtttca tcgcagactc
     7501 ggagctcatg ccgtagaaca gcataaattt ctcgccgttc tcctcaacga tgcgaccgcc
     7561 gctctcttcg tggcccgcca gcatgccacc aagcatgacg aaatcggcac cgccgccaaa
     7621 ggctttcgcc acatcgcccg gcgtggtgca gccaccatcg ctgacgatca ttccgcccag
     7681 accgtgcgca gcatcggcac attcgattac cgcagaaagt tgcggataac cgacgcctgt
     7741 tttgacgcga gttgtacaaa cagaacctgg gccaatgcca actttaacga tatcagcacc
     7801 ggagaggata agctcctcac acatttcacc agtcactacg ttaccagcac aaatggtttt
     7861 ggtcggccac gcttcacgcg ctttcgcaac gaactgcacg aagtgttcgg aataaccatt
     7921 cgccacgtca atacaaacga agtttaatgc cgggttcagg tcgagaatct gtttagtttt
     7981 ttcgaaatcc gcatcagacg taccggtaga aaccatcaca tgtttcagca catcagcgga
     8041 agaattgttg ataaacgctt gccactcttc gacagaatag tgtttatgca cagcagtcaa
     8101 aatatcaaaa gaagccagcg cagaggccat agaaaatgtg cctacggtgt ccatatttgc
     8161 ggcgataatc ggcacgccgg accagctctg acctgaatgt ttgaaggtga attgacgttc
     8221 cagttcaaca tcggaacggc ttttaagagt ggagcgttta gggcggatga gaacgtcttt
     8281 aaaacctaac ttcagatctt cttcaatacg catgtgcgga ttcctggggt taatggctat
     8341 aagggtaaga gcgcaactcc agtgacgcta tcatacgcac taatcaatgc ggcgcaagac
     8401 tgcgaaattg tcttttttta cgctacaatc ccataaattt acctggtaaa tagtgagcga
     8461 acgtttcatc aatttcgctt tcgtgcactc atttttaacg ttttgataat catgatgaat
     8521 tcccgggaat ataaattatg aggtatatag ttgccttaac gggaggcatt ggcagtggca
     8581 agagtaccgt tgccaatgcg tttgctgatc tcggaattaa cgtcattgat gccgatatta
     8641 ttgcgcgtca ggtggttgaa ccaggtgcac ctgcgctaca tgccattgct gatcactttg
     8701 gcgctaacat gattgctgct gatggaacat tgcagcgccg ggccttgcgc gagcggatct
     8761 tcgccaaccc ggaagagaaa aactggctta acgccctgct gcatccgctg attcagcaag
     8821 agacgcaaca ccagatccag caagctactt ccccctatgt actgtgggtt gtgccattgc
     8881 tggtagaaaa ctcactgtat aaaaaagcga atcgagtgct ggtggtggat gtcagcccag
     8941 aaacgcaact taagcgcacc atgcagcgcg atgatgtaac tcgcgagcat gtcgaacaaa
     9001 tccttgctgc tcaggcaacg cgcgaagccc gccttgccgt ggcagatgac gtcattgata
     9061 ataacggcgc accggatgct atcgcatcgg atgttgcccg cctgcacgca cactatttgc
     9121 agcttgcgtc gcagtttgtc tcacaggaaa aaccgtaatg cagacccagg tcctttttga
     9181 acatccacta aatgaaaaaa tgcgtacatg gctgcgcatt gagtttttga ttcagcaact
     9241 caccgttaat ttacccatcg ttgaccacgc tggcgcgctg catttcttcc ataatgtcag
     9301 tgaattactg gatgttttcg agcgcggcga agtccgcact gagctgttga aagaacttga
     9361 ccggcagcaa cgtaaactcc agacctggat tggcgtgcct ggcgtggacc agagccgtat
     9421 tgaagcatta attcagcagt taaaagcggc ggggagcgta ttaatttccg cgccgcgtat
     9481 cgggcaattt ctgcgtgaag atcgtttgat tgctctggtg cgtcagcgac tgagcatccc
     9541 aggcggctgt tgcagctttg atttacctac attgcacatt tggctgcatc taccccaggc
     9601 gcagcgcgac agccaggtag aaacctggat tgccagcctg aacccgctca cccaggcact
     9661 taccatggtg ctggatttaa ttcgccagtc ggcccccttc cgtaaacaaa ccagcctgaa
     9721 tggtttttat caggataacg gtggcgatgc cgacttgctg cgcctgaatc tgtcgctcga
     9781 ttcacagctt tatccgcaaa tttccggaca taagagccgt tttgccattc gttttatgcc
     9841 gctggacact gaaaacggac aggtaccgga acgtctggat ttcgaactgg cctgttgcta
     9901 aggagtaaaa atgtcagaaa ctattacggt gaattgccca acctgcggga aaacggtggt
     9961 gtggggtgaa atcagcccgt ttcggccatt ttgctccaaa cgttgtcagc tgatcgacct
    10021 cggagaatgg gctgctgaag aaaaacgaat ccccagcagt ggcgatcttt ccgaaagcga
    10081 tgactggagc gaagagccaa agcagtgaca tttgtctgat gccgcacgta ggcctgataa
    10141 gacgcgggca gcgtcgcatc aggcatcttg tgcaaatgtc ggatgcggcg tgaacgcctt
    10201 atctgaccta caaaaacaaa tttatcaatg agttataaat ccctgtaggc ctgataagac
    10261 gcgccagcgt cgcatcaggc atcgtgtgcg aatgtcagat gcggcgaaaa cgccttatct
    10321 gacatacaca cgcttaagct tcgcaattac ctgttcattg gctggcggaa actcatcggc
    10381 attaagaccg accagcgaca tccactcacc gggttgccct tctttacccc acggctcccc
    10441 ttcccagcgt tcgaccagcc aaaaccacag tgttatatgc ctgtccggga attcatattc
    10501 cagtttttca aatagcgaaa aatgttgggg ggtaatcccg acttcttcct gaagttcacg
    10561 caccaccgcc tgttccggcg tttcacccat ttcaatttta ccgccgggaa actccagttt
    10621 attcgccatg tgcgcatctg ctgcgcgacg cgttataaag atttcattgt tctcgttgcg
    10681 aataatacct accgcaattt gcagcttttt cattgtctta aacctataaa aaaggcgcag
    10741 aatcctgcgc cttttacttt aacagttagc ttttattgca ggcggccatg gcactgcttg
    10801 tattttttac cagaaccgca cgggcaagga tcgttacgtc ctactttgcg ctctccggtt
    10861 tgcgccgcca gtgcagctgc ggctgcagag tcgtcatcct gatggctaag ctgctgcatt
    10921 tgcgctaaac gctcggcttc catacgacgc tgttgttcca gctcctcaac ctcttcaggc
    10981 atacgtacct ggactttgct caacgtgctg ataacttcat attttaacga ctccagcatc
    11041 gctgcaaaca tggagaacga ttcacgtttg tattcctgct tcggatcttt ctgtgcgtag
    11101 ccacgcaggt ggataccctg acgcagatag tccatcgctg ccaggtgctc tttccacagg
    11161 gagtcaagcg tttgcagcat gacgcctttc tcgaagtgac gcatcatctc agcaccaacc
    11221 acttcttctt tacgctgata cacttcgatg gactgcgcca gaattcgctc acgcagcgtc
    11281 tcttcatgca gttctggttc tttatccagc cactcggcaa ttggcaaatc gaggtcgaaa
    11341 tcgttcttca gacgttcctg cagccccgga atatcccaca tttcttccag cgactgtggt
    11401 ggaatgtagg catcaatggt cgctttgaac acatcttcac gaatgctgtt aatggtttcg
    11461 ctcacatcgc tgacatccaa cagttcgtta cgctgggagt aaatggcgcg acgctgatcg
    11521 ttagccacgt catcatattc cagcagttgc ttacgaatgt cgaagttacg gctttcaact
    11581 ttacgctggg cgttggcaat cgctttagtc acccacgggt gttcaatggc ttcgcctggc
    11641 ttcataccca gtttacgcat catgccggat actcggtcgg aagcaaaaat acgcatcagc
    11701 gcatcttcca tcgacaggta gaaacgggaa gaaccagcat ccccctgacg accagaacga
    11761 ccgcgcaact ggttatcgat acgacgggat tcgtgacgct cggtaccgat gatatgcagg
    11821 ccacctgctt ccagtaccgc atcgtgacgt acctgccagt cggctttaat tttttcaatt
    11881 tgctctgcgg tcggattttc cagcgcggca acttctgcct gccagctacc accgagcaca
    11941 atatctgtac cacgacccgc catattggtc gcgatagtca ccgcagccgg ataacctgcc
    12001 tgagcaacaa tcgccgcttc gttggcgtgg aatttggcgt tcaggacgtt gtgcttaata
    12061 ccggctttgg tcagttcgtt tgacaccagc tccgattttt cgatggagat agtacccacc
    12121 agcaccggct ggcctttcgc agtacgttct ttgatatctt caatgatcgc ctgaattttt
    12181 tccgcttcag tcatgtagac caggtccggc agatctttac gaatcattgg acggttggtc
    12241 ggaacaacga cggtatccag cttgtagatt gagctaaatt cgaaagcttc ggtatcagca
    12301 gtaccggtca tccccgccag tttttcatac agacggaagt agttctggaa ggtgatcgaa
    12361 gccagcgttt ggttttcgtt ctggatctgc acaccttctt tcgcttccac agcctggtgc
    12421 agaccatcgg accagcgacg gccctgcatg gtacgaccgg tgtgttcgtc aacgatgata
    12481 acttcaccat ctttaacgat gtagtcgacg tcacgggtaa acagcgcatg agcgcgcagc
    12541 gccgccgtta cgtggtgcat cagcatgatg ttggccggag agtacagaga ctccccttca
    12601 tccatgatgc cctctttcac cagcagttct tcaatcagca ccagaccacg ttcggtcagg
    12661 ttcacctggc gagatttttc gtccaccgag aagtggcctt cgccctggaa ggtttcggag
    12721 tcttcttttt cctgacggat caggtgcgga ataattttat tcacgcgttt atacatttcc
    12781 gagctgtctt ctgccgggcc ggaaatgatc agcggtgtac gcgcttcatc gatcaggatg
    12841 gagtccactt cgtccaccag cgcatagtgc agtttacgct gtacacgttc ttcagggctg
    12901 aacgccatgt tgtcgcgcag gtagtcaaag ccgtattcgt tgttcgtacc gtaagtgatg
    12961 tcagctgcgt aagcttcgcg ctttgccggt gctggcatgc ccggcaggtt gataccgaca
    13021 gtcaggccaa ggaattcaaa cagcggacgg ttgttttcgg cgtcacgttg cgccaggtag
    13081 tcgttgacgg taactacgtg cacgccttta ccggttagtg cgttcaggta agcaggcagc
    13141 gttgcggtca gggtttttcc ttcaccggta cgcatttcgg cgatgcagcg ttcgttaaga
    13201 accataccgc cgagtaactg aacgtcgaag tgacgcatac caaagacgcg cttacttgcc
    13261 tcacgtacca cggcgaaagc ttccgggatc agattttcca gcacttcgcc tttttccaga
    13321 cgtgcacgaa actctgcggt tttccctttc agttcttcgt cggagagttt ttccatctcc
    13381 ggttccatgg cattgatgat gttgaccact ttgcgcatcc ggcgcagggt gcgatcgtta
    13441 cgactaccga aaactttagt taacaatttg attagcataa taaaatctca aacgccccgc
    13501 gttgcggagt taataaaatg aagtaaaggt ttattgttgt taggtgaggc gttgagggcc
    13561 agcacggatg ccttgcgcct ggcttatcca gacgggcgtg ctgaattttg cttgtggggt
    13621 aaaatgcgca taatcaatgc gataaccctt ttcagacggc gtgccttcct gggtcagcag
    13681 cgcgctgagc gtatccagta atgcaagatg ttgcgcctga agaggcaaag attcttcagc
    13741 aacgggcagt gtttgcggtg ccattgcgaa agaaagatga cggattaccg tgcgaatggc
    13801 atgttgatgc cagtaatcaa cggaatagtt cgaattcggg cggcgtgtgt tcgcttccag
    13861 caaggccaat tgaccaaagt taactttggc tgaaggctcg tggttgcggg ttgtcgcttt
    13921 tgcgggcgcg tttggttcgg cggcgttgct gagcgcaggc aaacctaaac tcgccgcaac
    13981 catccctaat aagagatgcg gccagaagta gcgtttacca aactgtcgcc agcgcgtcag
    14041 tattccactc acgttattgc catcccgatc gtattcaaac gtatttagcg cacaaatctt
    14101 atcattaaag cctgcagtcc acagcacgaa tgaagataag ttgtgcggaa aagtgcttaa
    14161 gaaaacagcg ttcgcaccgt tttttatagc tcttataaaa ttcaaaaaaa aacgactggt
    14221 tcacctggcc ggagagagtg ccagatttgc cagtcgaatt ttatacgaca gtataaatgt
    14281 cgttatgcca gtacagctga aggcgctttg aaggccaacg gcagttctgc gtcgtcctgg
    14341 aaggtcacat attcccagac ttcctgtttc gccaggacag cctgcagcag tttgttattc
    14401 agtgcatgac cggatttata agcggtaaat gcaccaataa tattgtgacc acacatgaac
    14461 aagtcaccga tcgcatcgag cattttgtga cgcacaaatt cgtcttcaaa acgcaggccg
    14521 tcttcgttca gtacgcgata atcgtcaaca acgatggcac aatcgaagct gccgcccagg
    14581 cacaaaccac gggactgcag atattcgata tcacgcatga aaccgaacgt acgcgcacgg
    14641 ctgatctggc gcataaacgc atcagcggag aagttcatcg catagcgctg gttgctggaa
    14701 tcaatagccg gatggttaaa atcgatggtg aaatccagcg aaaaaccatt gtacggctta
    14761 aattcagccc acttatcgcc atcttcgaca cgaacagtct ctttgatgcg aacaaatttt
    14821 ttggcgcagt tcaactcgtc gataccggcg tcaagcagca ggtatacaaa cggagcggcg
    14881 ctgccgtcca tgatcgggat ttccggcgcg ttaacttcga taacaatgtt atcgatgccc
    14941 aggcccgcga gagcagcatt gaggtgctct acggttgaaa tccgtacatc atgctcgttg
    15001 accagacacg tacagagcat ggtatcacgc acagatttgg catcggccgg gaaatctacc
    15061 ggtggattca agtcggtgcg acgatagatg accccggtgt tggccggcgc agggcgtaac
    15121 gtcagggtga ctttcttgcc ggtatgtaaa ccgacacccg tcgcctgaac gatacgttta
    15181 agtgtccttt gtttgatcat cgtattatct cgccaaatta cctatccaac cgaagtgtac
    15241 tatacattcg gcgggccagt ttagcacaaa gagcctcgaa acccaaattc cagtcaattc
    15301 ttaatcagct tgcttacgca ggaatgctgg gatatccaga taatccggct ctttcgcagt
    15361 ttgcggcgca ttgtcattca cgactttagc aactggcttc tgctcctggg tcagcggagc
    15421 catcccatgc tgctggtagc gatccatcac tggctgctga acctgcttat tggtcaccag
    15481 agtgatttca ggacgtttgt ccatgccgat acctgtcgca acaacagtta cgcgcagctc
    15541 gtcattcata tccgggtcaa gagaagtacc gataaccaca gtcgcgttgt cggaagcaaa
    15601 tgcacggatg gtgttaccta ccgtttcgaa ctcatccaga cgcaggtcga agcccgctgt
    15661 gatgttaacc agcacgccgc gtgcgccaga caggtcgata tcttccagca gcggagaaga
    15721 aatagccatt tcagcagctt cttccgcacg gtcttcaccg ctcgccacgc cagaacccat
    15781 cattgcgtag cccatttcag acattacggt gcgtacgtct gcaaagtcca cgttcatcaa
    15841 acccggacga gtaatcagtt cggcgatgcc ctgaacagcg cctttcagta catcgttcgc
    15901 tgcgccaaac gcatccagca gggagatacc gcggcccaga actttcagca gtttgtcgtt
    15961 cgggatagtg atcagagagt ccacatgctt ggacagttca gtgatcccct gctccgcgaa
    16021 tgccatacgc ttcttgcctt caaagttgaa aggcttagtg acgacagcaa cggtcaggat
    16081 acccaaatct tttgccactt cagcgacgac tggtgctgca cctgtaccgg taccaccacc
    16141 catacccgca gcaataaaga ccatgtctgc accttccagc gccgcacgca gtgcatcgcg
    16201 atcctcatca gccgcattgc ggccaacttc tggattagcg ccagcgccca gtcctttggt
    16261 gataccgcta ccgatttgaa tcgtctgtcc aaccgctgtt ttacgcagcg cttgtgcatc
    16321 ggtatttacc gcgaagaatt caacaccttc aatacgctcg cgcaccatgt gttcaacagc
    16381 attaccgccg ccgccgccga cgccgatgac tttaatcacc gcgtcattgg taagttccat
    16441 tggttcaaac atagtttctc tccgatttgt gcctgtcgcc tgaggccgta atcatcgtcg
    16501 gcctcataaa aattaaaact cttttcgcag ccaactattg agtcgcttga tccacgagcc
    16561 aactgatgct gtaacacgtt tttctacttc agcttcaccg ttaagatgtg actctttccc
    16621 atagtgaagc aatcccaccg ccgtcgaata atacggctcc tgagcataat ccgttaaacc
    16681 ggtaatgttc agcggcgcgc cgatacgcac ttgcgtatga aacacgcgct gagcacaggc
    16741 tgcaagacct tcgatctgcg ctgcgccacc ggttaataca atgcctgccg ccaggtgatg
    16801 tttaacccct tgttggcgaa gcttttcctg caactgcaat atctcttcgt tgaccaggtt
    16861 gagcagctcg gtatagcgcg gctcgatcac ctctgccagg gtctgacgtt gcagactccg
    16921 cggcggacga ccacctacgc tcggcacttc cacgctctca tcttttccaa cgatggaacc
    16981 cagcgcacaa ccgtggcgaa ctttaatcgc ttcggcgtcg cttggcggcg tgccaaaggc
    17041 gtaagcgata tcactggtca cgacattgcc agcataagga attaccttag tgtggcgcaa
    17101 tgccccaccg gtataaacgg cgatatccat tgtaccacca ccgatatcga cgacgcagac
    17161 acccagttca cgttcatctt ccgtcaatac cgaataactt gatgccagtc cggcaaatat
    17221 cagttggtca actttcagcc cacaacgttc aaccgctttg acgatgtttt tcgccatatc
    17281 gttgtgacat gtgatcaggt gcacttttgc ctgcatccgc acgcccgaaa gtcctaccgg
    17341 attcttgatc ccttcctgat agtcaatcgc atactcttgc gggatcacat gcagcacacg
    17401 atgctcatcg cgcacacgca ccgatttcgc ggtatggacg acgttttcca catcttcttg
    17461 cgtcacttct tcttcagaaa taggcaccat accaatttca ttctggcagc tgatgtgctt
    17521 accagaaagc gccagatata ccgaagagat ctgacaatct gccatcaatt ctgcctggtc
    17581 aatggcgcgt tgtacgcact tgaccacgga ttcgaggtcg ttcaccccgc ctttatccat
    17641 accacgcgac gggcagctgc ccacgccaat gatattgacc ataccgtcgg gcagaacttc
    17701 ccctactaaa gcggcaacct tcgcggtacc aatctccagt cctactacca gttttctgtc
    17761 cgtcgccttg atcattgttg ttctgcctgt gcctgatttt gttgctgagt agattcctct
    17821 ggcggcaagg gcgcccagcc tactgccgct ccagagtcat aacgcaaatc aacgtagcta
    17881 atccgtttgc catcggtttg cgcctgctgc tgtaaaaccg gataaagttc tacaaagcga
    17941 gccaaacgtt tcatcgtatc gccccggcca agattgagct taatatcgtt attcagcgtc
    18001 aactgccagg aacgccgcgc ggtcatcgcc gcttccttca gagtaaatct gtcctttgcc
    18061 agcatctgcc ccatttcgcg atagccctgc aacacttcat tggcgctgcc ttccgggcca
    18121 tacagcattg gaagcacctg cttgctggtg cgttctggcg gcacgctgaa ggtatttcct
    18181 tccgcgtcta ccatatgttg atcattccac cgcgcaatcg gcacatattc aaccagatga
    18241 atcttcaatt catcaggcca ctgctttctg acgctcacct gcttaatcca cggcaggcgt
    18301 tgttctattt gcgtctggat gatgttgaca tcctgggtca taaaggtacc cggctcaccc
    18361 aatgccagga tcgactgccg gatatcgtca ttacgtgtgt aatggcgttc accggtcaac
    18421 accagctttg agagcggcag gcgttgcgca tcttccatcc agcccaacac gacccagccg
    18481 ctcaccaaca ctgtcgttaa aacggtcagc aggaaaagga tccccgccag acgcgttcca
    18541 ttattgcggc gagaagaaac ctcttcttcg ctgtttcgcg tgttcagagc agcctgcgac
    18601 atattagtcc gccagttcca gaattcgtac taccaactgc gagaagctca tacctgcctg
    18661 acgtgccgcc atcggcacca ggctgtggct ggtcataccc ggtgaggtat tggcttccag
    18721 cagataaaac tgtccatcgc tgtccagcat aacgtcaata cgtccccatc ctttgcaacc
    18781 taacgtcgtc catgctttca gcactaatgc ctgcaaattg gcctcttgtg acgcttccag
    18841 acctgcgggg cagaaatact gtgtctcatc agagagatac ttcgcctcat aatcatagaa
    18901 ggttccggac ggttgaatac gtattgacgg taaaatttct tcaccgagta tcgcaaccgt
    18961 gaactccggc ccacttagcc atttttcaat caatacttct tcatcgtgct gaaatgccaa
    19021 tcttaatgca tcttgtagag cattttctgc tactactttt gacattccca cactggaacc
    19081 ttcgcggctc ggcttaacga taaccggcaa acccagagca gaaatttctg ctaactgctt
    19141 atcgctcagg cctttttcaa actctgcgcg ggttaacgct acccacggcg cgaccggtaa
    19201 accggcacct tgccatagaa gtttgctgcg tagtttatcc attgaaagcg cagatgccat
    19261 cactccgctt ccggtataag gcaagcccat cagctcgagc atcccctgca gcgtaccatc
    19321 ttcaccgccg cgaccgtgta gcgcgataaa cactttctga aagcccatcg acttcagttg
    19381 cgtcacgtcg acttctttcg ggtcgacagg atacgcgtca ataccgcctt cacgcagtcc
    19441 ggctaacacc gctgcgccag aattcagaga aacttcccgc tcagcggagg tcccacccaa
    19501 caggaccgcg attttatcag tcatgttgtt cttcctccgg agtttgcggc ttcagtttga
    19561 tttcagctaa agaacgagca atttttccaa tattaccagc cccctgaacg agaatcaggt
    19621 cgttaccggt taataccggt gccagcatct cggctacccg cgccggatcc ggcaccagaa
    19681 tgggatcaat tttcccacgt ccacgaattg tgcgacacag cgaacggctg tccgctcccg
    19741 gaattggcgc ttcgccagcc ggatacactt ccagcatcaa cagggtatca acctgcgtca
    19801 gcacattggc gaaatcatca tacaggtcgc gcgtacgggt aaaacggtgc ggctgaaaca
    19861 gcattaccag gtttttatcc ggccagcctc cgcgcgccgc tttaatggtg gcgtccactt
    19921 ccgtcgggtg gtggccgtag tcatcgacca gcattgccgt accgctttta ccattcactg
    19981 gctccagcgg gaattcaccg aggaaatcaa aacggcgacc agtcccctgg aagctttcaa
    20041 gcgcccgcaa aatagcctcg tcgtcaatgc cctcttccgt agcaaccgca accgcagctg
    20101 cggcgttcag cgcgttatga cgacctggcg cattcagggt gacgcgcatc ggctctttgt
    20161 cctggcgcag cagcgtaaag tgcccctgcg ggccaatctg ctgataatct tctacacgca
    20221 cgtcggcatc ttcgctgaag ccgtaagtcg tggtctgacg ccccactcgc ggtaacaatt
    20281 cgcggatcac cggatcatca acacacatca ccgcacgacc gtaaaacggc aggttgtgca
    20341 gaaaattaat aaaagtctgt tttaaattct caaagtcgcc ctggtaggta tccatgtggt
    20401 cggcttcgat attggtgaca atcgccacca tcggttgcag atgcaggaac gatgcatcac
    20461 tctcatctgc ttcggcaatc aggtaccgac catgccccaa acgcgcatga acccccgccg
    20521 cttttaccag cccgccgtta acgaaggttg ggtcgagccc cgcttctgcg tagatgctgg
    20581 aaaccatcgc ggtggttgtc gttttgccgt gcgttccggc aatggcgatg ccatgacgaa
    20641 aacgcattaa ctcagccagc atttcggcac gacggatcac cggaatacgc gcttcatgag
    20701 cggcgacaat ttccgggtta tcggcagaaa tcgcgctgga aacaacgacc acgctggcat
    20761 cacgtacgtt ttccgggcga tggttgaaat aaatcgtcgc acccagattc attaactgct
    20821 gcgtgaccgg atttggcgct aaatcggaac cactgatctg ataaccttca ttggccagaa
    20881 cttcggcaat accgcccata ccggcaccac caatgccgac aaaatgtatg tgccgaacgc
    20941 gacgcatttc gggcacgatg gaacgcagtt ttgccaattg ttgtgtattc attctttacg
    21001 ccattaactt cttaaattca tacgatgcaa aaggcatcgc tacaattacg cccgggcaac
    21061 ccggctcact tcatttgcca ctcgctcggt ggcatccgga atggatgcag cgcgggcgcg
    21121 ttctgccatg gttaataagg tttctcgcga ccacccggcc agggtgttgg cgacagcatc
    21181 cacgctaagc tgtggctgct cgataatttt ggctgcgccc gctttttcca gcggtagcgc
    21241 attccagtat tgctggcggt ctttatgttg aaacggcaca aacaacgccg gtagtcctgc
    21301 cgcggcgatt tcactcaccg ttaacgcacc ggagcggcaa acgacgacat ccgcccacgc
    21361 atacgccgcc gccatatcat caataaattc cgtcacttta tgctgcggtt gccccgcttc
    21421 ggcatacgcc tgttcaacgg attgttgcga acctttgccg ctctgatgcc agatagtgac
    21481 tgaatcaccc agtttcgcag caacctgcgg cattgtctgg ttaagaatgc gtgcgccctg
    21541 agaaccaccc actaccagca cacgaaccgg accttcacgt ccagccaaac gttgctgcgg
    21601 caacggcagc gccaacacat cggtacgcac cgggttaccc actacttccg cattagggaa
    21661 agcacctgga aacgcctgca tcactttggt ggcaatcttc gccagccatt tattggttaa
    21721 gcccgcaata ccgttttgtt catgaagtac aaccggaatg cctaacgacc acgcggccag
    21781 accacctgga cctgacacgt agcctcccat accgagcacc acgtcaggtt tgtacgcttt
    21841 cataatcgcc cgcgcctgac gccaggcgtt gaagatacgc agcggggcag ctatcagtgc
    21901 ttttatacct tttccacgca gaccagagat acgaatgaaa tcaatttcga tgccatgttt
    21961 tggcactaag tccgcttcca tacggtcggc agtccccagc cagcgaactt gccaaccctg
    22021 agccattaga tggtgcgcaa ccgccagtcc cgggaataca tgtccaccgg ttccgcctgc
    22081 catcaccatt aatcgctttc cttgaccact catcgtgaac ctcgtacaaa cgcctgcgct
    22141 ttctccagac gcgtttcata atcaatacgc aacagcatca tgatggctgt cgacataatc
    22201 agtaagctcg aaccaccgta actgatcagc ggcaatgtca gacctttggt cggtaacatc
    22261 cccgccgccg cgcctacgtt aaccagcgcc tggaagctaa accagatgcc aatagaacag
    22321 gcgagaaaac cggaaaaacg gtggtcaatt tctaatgctt tacggccaat cgacatcgcg
    22381 cgaaaagcga cgaagaatac cattaaaagt gccagcacca caccgacata ccccagttct
    22441 tcgccgataa tggcgaaaat aaagtcagtg tgcgcttccg gcagatactc cagtttttgt
    22501 accgagttac ctaaaccttg cccccaaagt tcgccgcgac caaacgccat cagcgattgc
    22561 gttaactgat agccgctgcc aaagggatct tcccacgggt tccagaatgc ggtaacacgg
    22621 cggatacggt acggttcggc gagtatcagc aacacaaccg ctgaaatgcc cataccgata
    22681 atggcaatga actgccacaa tttcgctccc gccaggaaca acatcgccag cgtagtcaca
    22741 aacaacacca ccaccgtacc aaggtctggc tgtgccagca gtaacactgc caacaccaga
    22801 atcacgccca tcggtttcag gaagccgcgc aggttattac gtacttcgtc gcctttacgc
    22861 accagatagt tggcgatata gcaaaacagc gacagttttg tcagctccgc aggctggata
    22921 cgcagcaaac cgagatcgat ccaacgcgat gcccctttaa ccgagctacc cactaccagg
    22981 acgatcatca gcaggatgat agatccgagc agcatcgtgg cactgtagcg ttgccagaac
    23041 tccatcggca gacgcagcgt aatgatcgcc agaataaacg ccaaaatcag atagacacca
    23101 tcacgcttcg cgaagaagaa cggatcgttg gttaagcgtt gccctatggg cattgacgcc
    23161 gaggtcacca tgataaagcc aatcgccgcg aggccgaagg tcagccacag taaggtgcga
    23221 tcgtacatga tcaggctgtc ggtatctttt tcccgcgagc ccatcaccca gccctttagc
    23281 gccgtggaga tccagaccag gatactgaat cctggcaggc gcggcatttt caggcgaggg
    23341 agagataaac gcatcaacct aactccttcg ccagacgggc aaactcattg cctcgttgtt
    23401 caaagttctt gaactgatca aggctggcac aggctgggga gagcagaacc atatcgcccg
    23461 gctgaacacg cggagccagc aagcgcatcg cctgttccat agtttcggtt tgttctgcca
    23521 cttccgggcg tagcgccgcc agctgcgcgc cgtcacgacc gaaacaatac agacgtacgt
    23581 tatcgccatt caggtaacgc gccagtgggc taaagtccgc cgatttacca tcgccaccca
    23641 gcaacaaatg cagtgtgccg tctacgtgca ggccattcag cgccgcttcc gtactgccga
    23701 cgttggtcgc tttcgaatcg ttaatccaac gtacgccgtt atgctccagc acaacttcaa
    23761 agcgatgcgg cagaccagtg aatgtggtta acgctttcag gctgctggca cgcggtaacc
    23821 ctgcagcatc tgccagcgcc agcgccgcca gcgcattggt gtagttatgc tgcccggaaa
    23881 gtttcatctc tttcacattc agcactttct cgcctttaac ccgcagccag gtttcgccct
    23941 gctgatgatt caggtgatag tcacccatgt tgacgccaaa gctgacgcag cgttcatccg
    24001 caccgcgaat cggcattgtt aaggcatcat cagcattaac cacgcaaact ttcgcgtttt
    24061 cgtaaatgcg cagttttgct gcacgatact gttgtaaacc aaacggatag cgatccatat
    24121 gatcttcagt cacgttcaga atggtcgctg ctaccgcctg taagctggag gtggtttcca
    24181 gctggaagct cgacagttcc agcacgtaca gttcacactc atcatccagt agcatcaacg
    24241 caggcaggcc aatattgcca cccacaccaa cgttaacccc cgccgctttc gccatttcac
    24301 ccactagcgt ggtgaccgtg cttttgccgt tagaaccggt aatcgccaca atcggtgctt
    24361 gtgcttcgcg acagaacagc tcgatatcgc caacgatttc gattccggca tcagcggcag
    24421 cgcttaagga tggatgcgcc agtgcaatac cgggactggc gacaatcaga tctgccgcca
    24481 tcagccattc atcattcaga ctgcccgtgt ggcgttctac ggcttcgggt aatttatcca
    24541 ggccaggcgg tgtcatacgc gtatccataa cgcgcggcgt cacaccgcga gcgaggaaaa
    24601 agtccacgca ggaaagcccg gtgaggccca ggccgataat gacgacattt ttaccctgat
    24661 aatcagccat gattaacgta ccttcagcgt tgccagacca atcagaacca gcatcagcga
    24721 aataatccag aaacgcacaa tgacgcgcgg ttccggccag cctttcagtt catagtggtg
    24781 atgaatcggt gccatgcgga aaatacgttg tccgcgcagt ttaaaggagc cgacctgcag
    24841 gatgacagaa agcgtttcta ccacgaacac gccccccata atcaccagca ggaattcctg
    24901 acgtagcagt acggcgataa tgcctaacgc accacctaac gccagcgaac ctacatcgcc
    24961 cataaagacc tgcgccggat aggtgttaaa ccacaggaag cccagtcctg ccccgactat
    25021 cgcggtacag acaataacca gttccccggc gtgtcgcaga tacggtatat gcaagtagct
    25081 ggcaaagttc atattgccgg tcgcccacgc caccagcgca aaaccaccgg cgacaaatac
    25141 ggtcggcata attgccaggc cgtcgagacc atcggtcagg tttaccgcgt tgccagtacc
    25201 cacaatgacg aagtaagcca gcagaatgta gaacagcccc agctgcggca tcacatcttt
    25261 aaagaatggg accaccagct gcgttgcggg cgtgtctttg ccggcaaggt acagggcgaa
    25321 ggcgacaccc agcgcaatga ccgacatcca gaaatacttc caacgagcga tcaacccttt
    25381 ggtgtcttta cgcaccactt tgcgataatc atcaacaaag ccaataacac cgtaacctac
    25441 cagcaccacc aacacgcacc agacgtacgg attggacggg taagcccaca gcagtacgga
    25501 gatcacaatc gccgtcagga tcataatccc gcccatggtc ggcgtaccgc gcttgctgaa
    25561 gtgtgattca ggaccgtcgt tacgcaccac ctgaccaaag gaaagttttt gcaaatgagc
    25621 aatcatacgc gggcccatcc acaatgagat gaacagcgcg gtcagcaggc tgacgatggc
    25681 gcgaaacgtc agataggaaa agacgttaaa gccggaataa tatttgacca aatgttcggc
    25741 cagccaaact aacatgtccc attctcctgt aaagcgcgta ctacctcttc catggcggca
    25801 ctacgtgaac ccttaactaa aatcgtaatt acctgttgct cagcaatcag tgatttaaga
    25861 cgcgtaatta acgcagtttt atcagcaaaa tgttcgccaa cgccgctggc ggtgctgata
    25921 gcatggcttt gtttacccac gcttaacacg cggtcaatac cagcagcttt tgccgcctcg
    25981 cccacctgta catggcaggc ttcgctttca gcgcccagtt ccgccatatc gcccaccacc
    26041 agcacgcggt agcccggcat ttcagccagt acctggactg ctgcagtcat tgaaccgaca
    26101 ttggcgttgt aggagtcgtc gagcagcaac tggttttctg ccagttggat ggggaacaga
    26161 cggcctggaa cagctttcag atttgccagc cccgctttga tagcatcaag cgttgcgccc
    26221 acggacatgg agagcgcagc ggctgccagc gcattcgcaa tattgtgacg ccccggcaac
    26281 ggcagcagaa catcgacgct accggttggg gtttgtaggg taaattccgt accgtgcgag
    26341 gtcacatgga tattggtggc ggtgaaatcg ctgttggcgg cattgggtga gaaacgccac
    26401 actttgcgtg agccaattac gctctgccag ttcagccagt cgttgttgtc ggcgttcata
    26461 atggcgatac cgttttccgg caggccgcta aagatttcac ctttcgcttt cgcgacaccc
    26521 gcaagcgagc caaaaccttc cagatgcgcc gctgccaggt tgttgaccag cgcagcttcc
    26581 gggcgagtca gactcacagt ccaggctatt tcgccctgat ggttcgcgcc aagttcaata
    26641 actgcgtaat cgtattccgg cgttaagcgc aacagcgtca tcggtacacc gatgtcgttg
    26701 ttgagattgc ctgccgtata aagcgtgttg ccgcactggc ttaaaatcgc cgccgtcatc
    26761 tctttaacgg aggttttgcc ggaggacccc gtcagagcaa ccacgcgcgc cggaacttgc
    26821 tggcgaaccc atgcagccag ttcaccaaac gccagacgcg tatccttgac gattaactgc
    26881 ggcaggtcga tgtccagcgg acggctaacc agtagtgcgc ctgcgccgcc agctttcgcc
    26941 tggtcggcaa aatcgtgggc atcaaaacgt tcgcctttca gggcaacaaa caggcagccc
    27001 ggcgtcagtt ttcgggtatc agtggttaca gcatcaaggg tgatatctgc accttgcagt
    27061 tcaccgttga gaatgtcggt aagttggcta agggttacgc taatcatgca atcaccccca
    27121 gcagacgcgc caccgtgacg cgatcggagt agtccagacg ctgattgcca acaatctggt
    27181 aatcttcatg gcctttgccc gcgaccagta ccacatcatt ctctttagcc tgcataacgg
    27241 cgcaagtcac cgcttcagca cggccttcca tcactttggc atgtccggca tctaacattc
    27301 ccgccagaat atcgttgatg atggcacgcg gttcttcggt acgcgggtta tcgtccgtca
    27361 ccaccgccac gtcagcaaac tcttcggcaa ttgcgcccat cagtggacgc ttacctttat
    27421 cgcgatcgcc accacagcca aagacacacc acagcttgcc cgcacagtgc agacgcgccg
    27481 cctgtaaggc tttttccagt gcatccggcg tatgcgcgta atccaccacc accgtcggtt
    27541 tgcctggcgc agtgaacact tccatacgtc cgcaaaccgg ttgcagacgc gcggcggttt
    27601 tcagcagatc agccagtgga tagccgagtg ccaacagtgt cgccagcgcg agcagcaggt
    27661 tgctgacgtt aaaagcgccc atcagatggc tttcaatttc gccatcgccc caacttgagc
    27721 taaagcgaat cgtcgcaccg ctgtcgtgat agttcacttc ggtcgctttc aaccagcgtc
    27781 cgtgacagtt cggattgata tgatcttcca ttgataccgc aaccgcgtcc ggcagttttg
    27841 ccagccagcg gcggcccact tcatcgtcgg cgttaataat cgcctgaccg caatgatgtt
    27901 cagaataaag cagccatttc gcagcttcgt agtgttccat atcaccatga taatcaaggt
    27961 gatcgcggct taagttggta aagaccgacg ccgcaaattt caatgccgcc acacggtgct
    28021 gtaccagccc gtgggaggaa acttccattg cgcaaaaagt cgcgccctga tccaccagcc
    28081 ccgccagctc atgctgaaca tcgactgccg aaccggtagt attttctgtc gggatcactt
    28141 tccccagcag accgttacca acggtgccca ttaccgcgct ggtttcgcca agcagttggc
    28201 tccactgcgc cagcagctgg gtcgtcgtgg ttttgccgtt ggtgcccgtt acgcctacga
    28261 gacgtaaatt gtcagagggt tcatggtaaa agcggcccgc cagtgcagat aaacgctcat
    28321 tgagctggct gagatagatg accggtacgc cgtgcatttc acggatttca ccgtcggtcg
    28381 cctcatcttt cgcctctgca ataatggcag ccacaccttg cgctatcgcc tgcgggatat
    28441 atcgacgccc gtccgcctga tgacctacta cagctacaaa gagatcgccc gccgcagcca
    28501 cacggctgtc gagtgtcatc tctcgcagtg ctcgcgaagg tgcgtctggc acccacggag
    28561 caagaaggtc gcgcaaatta cgatctgcca cctgtcccct cgccttgatt aatcacaaat
    28621 tcatttttat cgcccgttgt cagcgcatcc ggctcgatgt tcatggtacg caatacgccg
    28681 cccatgatgg caccaaagac cggcgcggaa acggcgccgc cgtagtattt acccgcctgc
    28741 ggatcgttga taacaacaac cagcgcgaag cgcggctgac tcgcaggcgc aacgcctgcg
    28801 gtataagcaa tatatttatt gatgtagcga ccgtccggcc cgaccttttt cgcggtaccg
    28861 gttttaatgg cgatacgata gcctttaatc gccgccttca cgccgccgcc gcccggtagc
    28921 gccacgcttt ccatcatatg caccacagtg cggacaatgg attccgggaa gacacgttca
    28981 ccgggaaccg gggggtcaac tttggtaatc gacagtgggc gataaatgcc gtagctgccg
    29041 atagttgcgt agactcgcgc taactgtaac ggtgttacca ttagcccgta gccgaaagag
    29101 aaggtggccc tctctatgtc agaccaccgt tgtttttgag gatataagcc actgcgttct
    29161 ccgaccaacc ccaaattggt cgcttttccc agtccaaaac gtgagtaagt atctactaac
    29221 gctgaggacg gcatcgctaa cgccagcttg gaaacaccga cgttactcga cttctgtaat
    29281 accccggtca gggttaattc gctgtagcgt gccacgtctt tgatttcgtg gccgttaatt
    29341 cgataaggaa cggtattgag taccgagttt tctcgcacca cgccacgttg caacgcggtc
    29401 atcaccacca tcggtttaac cgttgagccc ggttcaaaca cgtcggtgat ggtacggtta
    29461 cgcatcgcct ctttcggcgt gccgctcaga ttatttgggt tgtatgacgg gctgttagcc
    29521 atcgccagca cttcaccggt gttgacatcc accagcacgg cgctaccgga ctcagccttg
    29581 ttaaaggcca ccgcgttgtt cagttcgcga taaaccagcg cctgcaggcg ttcatcaata
    29641 ctcagcgcca agttgtgcgc tgcctggctg tcagtagaag aaatatcttc aattacgcga
    29701 ccatagcggt ctttacgcac aatgcgctca cccggctgcc cggtaagcca tttatcgaaa
    29761 ctcttctcaa cgccctcaat cccttgacta tcgacgttag taaagccgat gaggtgagca
    29821 gtcacttcgc cggacggata gtaacggcga gactcttcac gcagatgaat ccccggcagt
    29881 ttcagttttt tgatgtagtc cgccatgtca gggttcacct gacgcgccag ataaataaag
    29941 cgccctttcg ggttggcgtt aatgcgggct gaaagctgat ccagcggaat attgagcgcg
    30001 ttagccagcg ccttccagcg gtcaccgacg ctgataccgc cagcgtcatg cacttctttc
    30061 gggtcagccc aaatcgcttt taccggcacg ctcaccgcta acgggcgacc agaacggtca
    30121 gtaatcatgc cgcgggaggt ggaaacttgc tgaacgcgaa gagaacgcat gtcgccctct
    30181 ttcaccagca tatccgggga gataacttgt aaccacgcta cgcgtccgag cagaaaagcc
    30241 agcgccagga gaatacagcc gcataacaac gcaaaacgcc aactgataaa gttggcatgt
    30301 tcttcctgac gttttggttt ctgcgttttc gccgctgctt tcatgcgtcg cgtttatcct
    30361 tatttttgca ctacgatatt ttcttgtgac ggatcaacat gctgcatttg cagcttttcc
    30421 gtggcgatcc tttccacccg gctatggtcg ccgagcgcat tctcttcaag gatcaggttg
    30481 cgccattcaa tgtctaaagc atctcgctcc agcaccagtt gttcgcgctg agcggtcagt
    30541 aaacgggtat ggtgcgccgt ggttaccaca gtcaccgccg tcaaaataat gcaaatgaac
    30601 aggcagagtg gcagcttccc aaatcgcaaa agatcgtcac cgataacacc aggcaatgca
    30661 tggcgctcgt ggcttcccat cgatccttta actttgctta gagcttctgt cactctgctg
    30721 atcatgcgtt cgtcctctct gcaatacgca gaactgaact acgggcacga gggttctcag
    30781 ccacctcttc ttcgcccggc attaacttgc ctagtgctcg cagctgacgg ccacccagtt
    30841 ttttgagctg ctcttcagtc atcggtaacc ctgccggaac ttgcggaccg cggctgtttt
    30901 cacgcataaa acgtttcaca atacggtctt ccagcgagtg gaagctgatg atcgaaagcc
    30961 gcccacccgg ggccagcacg ttgagcgagc tttttagcgc ctgctctatc tcctccagtt
    31021 cactgtttac ccaaatgcgc accgcctgga aggtacgggt cgcgggatgt ttaaacttat
    31081 ctttcaccgg cgttgcagca gccacgactt ccgccagttc tttggtgcgg gtcatcggct
    31141 gttcgcggtt acgctcgaca atggcgcggg caatgcgttt ggcaaaacgc tcttcaccat
    31201 aggttttcaa tacccaggcg atatcggctt cttctgcggt ttgtagccat tcagcggctg
    31261 actgcccacg ggttgggtcc atacgcatgt ccagcggacc atcgcgcata aaggaaaagc
    31321 cacgttcagc atcatcaagt tgcggtgaag agacgccaag atcgaggaga atgccgtcga
    31381 tcttgccgat aagatcgcgc tcggcaacgt attcgcccag cgcggagaaa ggtccgtgga
    31441 tgatggagaa gcgcggatca tcaatagtct tcgcaacggc gatagcctgc gggtcgcgat
    31501 cgatcgccag caaacgcccc tcttcgccaa gctgcgagag gatcagacgt gagtgaccac
    31561 cgcgaccaaa agtcccatca atgtagatgc catcaggacg gatattgagg ccattaacgg
    31621 cttcatccag cagcaccgta gtatgtttat agttttccat cattttatag agacaagtcc
    31681 tgcagtcgct ccgataagtc tccggtagcc aactgctctg cgtcgatatc ttccttgacc
    31741 tgttgatgcc aggttgtttc atcccacagc tcaaacttgt tgaactgtcc aaccagcatc
    31801 acttcttttg tcagcccggc atgttgccgc agtactggcg cgattaacaa tcgacctgcg
    31861 ccatccatct gacattcgct ggcatgacct aacagtaggc gctgcacacg gcgctcaacc
    31921 gggttcatgc tcgacagacg cgataatttt tgctcgataa tttcccattc aggcaggggg
    31981 taaagcagca ggcacgggtg ataaatgtca atggtgcaaa ccatttgacc ggcagcgttc
    32041 tcaagcagct gttcccgata acgggtaggc actgataagc gccctttgct gtcgagattg
    32101 actaacgttg ctccccggaa catgccagcc tcacccctta ttcccacttt gccccacaat
    32161 ttcccactga aaggagttta cggagctgag gaaaagcttg tcaagccagt cacaaggcat
    32221 accgaggtaa aaaacccgat gtttacggct aatatcagcg ttgtttattt tacgtccaac
    32281 gtgcgaagca aattaacgtt atgaatagct gtaagaaaaa atccctcatc gtcatcactg
    32341 ttttaaaatg actcactatt ttccacagca aatataaagt gtcagtttgc gacgcgagcg
    32401 gcattttagg acatatcttc cccggttaac agtccttgtt acgtctgtgt ggcgcttgat
    32461 gccagcgggt ttgcgcaaaa gcaaagaaaa gtgtttgtta aatcggcaat tcatcgccgc
    32521 atgtaacaaa ataatgcaaa aacgcgctgt cgatatcact taattattgt gaatttcagc
    32581 tggtgagtta attaacgggc gggtttaaaa gcatttatca ataatagtca ggaatagtct
    32641 tatttacttt aagcatattg atgtccagtc ccgtactcta cgcgccagag ggaaattcac
    32701 ctggcgcgta tttttgttcg cggcttagct acggctgagc acgccgcggc gatagagatt
    32761 acgtttaatg cgcgttaaac caggttttgg cttacgcggt tcgtccaggc ttgccaggac
    32821 aatctccagc acacgctctg cgacatcgcg gtgacgttga gccactgcca gcaccggaca
    32881 ctgtaagaag tcgagcagtt cgttatcgcc aaaggtggca attgccaggt cagaaggcag
    32941 tttgccgtcg cgacgcagcg tgacatccat cactccttgc aacaacgcaa acgacgttgt
    33001 gaacagcgcc tgcggcatcg gatgcgtttc cagccatttt tcgaataact gggcagccgc
    33061 ctcccgctca tagctgttgg catacaggaa atgcacttcg cgcggatcat ctttccaggc
    33121 agtacggaaa ccttgttcac gcaggaagct gacagaaagc tccggtagcg caccaagata
    33181 aagcaccgtc tcggcgggaa acttacgtaa ctcttccgcc agcatttcgg catcatcctg
    33241 atcggcacca accacgctgg tgaagtgttc acgatcgagg gcgcggtcca gcgcgacaat
    33301 cgggaacggg tcgttagccc agcgttgata aaaaggatgc tcaggaggca acgacgtcga
    33361 aacaataatg gcatcaacct gacgctgtaa aaggtgctca atgcaccgca tttcgttgtc
    33421 tggctgatct tctgagcagg caatcagcag ttgataaccc cgttgccgcg cctggcgttc
    33481 aagatagtta gcgatgcggg tatagctggt gttctccaga tcggggatca caagaccaat
    33541 agaacgtgtg cgtccagcac gaagcccagc tgccacggcg ttcgggtggt aattgtgctc
    33601 acgcaccaca gccatgactt tttcaacggt tttgtcgctc acacggtatt gcttcgcttt
    33661 gccgttaata acatagcttg cagtggtccg cgacactccc gccagccgag cgatttcatc
    33721 cagtttcaca attgcccctt gcgtaaaatg taaaaaccat agccattgta ctggtatggg
    33781 ttaaattcat taacatctaa gcgcagaaaa ttaactgcgg caactgcttt tattccaggt
    33841 tcccactgat ttcgcaaaaa aagcccaacg tgacatgttg ggctgtaaat tgcgcattga
    33901 gatcattctc aacgcattat tttatcgccg cgcgaaagtc cgaccacacc agagcgagca
    33961 acctccacaa ttttcgccac atcgcgaatc gatgctaaaa atgcatcaag cttaccgctg
    34021 gtgcctgcta attgaacggt ataaagcgag ggtgtgacat cgataatttg cccacggaat
    34081 atttccgtat tacgtttcac ttcgtcacgc ccgtaaccgc tggcctgaat tttcaccagc
    34141 atgatttccc gctcaacatg cgcgccctgc cccaactcac tcacgcgcaa gacatcgacc
    34201 agcttgtgta attgcttttc gatctgctca agtacttttt catcgcccac ggtctggatg
    34261 gtcatacgcg ataatgtcgg atcgtcggtt ggcgcaacgg tcaggctttc aatgttgtag
    34321 ccacgctggg aaaaaaggcc aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag
    34381 actgataata tccggcgcat aatcaggttc tctccgtttt gcttaaccac atttcatcca
    34441 ttccgccccc gcgaatctgc atcgggtaga cgtgctcgct gccatcgacg gtaacatcaa
    34501 caaacaccag gcgattattg cgcacctgtt ccagcgcctc gctaagtttg ctttccagct
    34561 catgcggatg agaaatctgg atcccgacat gcccataggc ttccgccaga cggacgaaat
    34621 cgggtagcga ttgcatataa gattgtgaat gacggccgga atagatcatg tcctgccact
    34681 gcttcaccat ccccagatag cggttattga gattcaccac cagtacgggc aactcgtatt
    34741 gcaacgcggt agacagttcc tggatgttca tctgaatact gccgtcgcca gtgacgcaaa
    34801 ccacggtttc ttctggcaac gccattttga cgcccagtgc cgcaggtaaa ccaaaaccca
    34861 tcgtgccgag gccaccggaa ttgatccagc gacgcggttt gtcgaatgga taataaagtg
    34921 cagcaaacat ctggtgctgc ccgacatcgg acgtcacgta agcgtctccc ttcgtcaacc
    34981 gccaaagagt ctcgatcacc gcctgcggtt taatcttttc actgtgagtg tcatatttca
    35041 ggcactgacg agcgcgccac tgttcaattt gctgccacca gtcgcggatc tcatccagtg
    35101 gttgatgggc ggattcttgc gacaagagtt caagcatttg ttcgaggacc tggcgagcat
    35161 cccccacaat cgggatatcc gcagtcacgg ttttagaaat ggaagtagga tcaatatcga
    35221 tatgcagaac agtggcattt gggcagtact ttgccagatt gttcgtcgtt cggtcatcaa
    35281 atcgtacccc gacggcgaaa atcacatccg cgttatgcat cgtcatattg gcttcgtagg
    35341 taccgtgcat tcccagcatg cccagtgcct gacgatgcgt tgccggaaac gcccccagcc
    35401 ccatcaatga gcaaacaacg ggcagattca acgcctccac cgtttctttc aactgctgat
    35461 ggcagcccgc cgtgattgcc ccaccgccta cgtagacaac cggttttttt gccgctacca
    35521 gcgtttgcag agcacgctta atttgccctt tatgtccggt agtagtggga ttgtaagaac
    35581 gcatactgac cgactccggc cagacatagg gtaatttgtt cgccggatta agaatatctt
    35641 tcggtaaatc aacgactact ggtcctgggc gaccacttgc cgccagccag aaagcctttt
    35701 tcagcacctg cggaatgtct tccgtttgct taaccagaaa actgtgttta accaccggtc
    35761 gcgaaatccc caccatgtcg cactcctgaa aggcatcgta acctatcaac gaggtcgcta
    35821 cctgcccgga aaggacaact aatggaatgg aatccatata agcggtggcg atgccagtaa
    35881 tcgcattggt cgcccctgga cccgacgtta ccagcacgac gccgacttcc ccggtcgcgc
    35941 gcgccaggcc atcggccata tgcaccgccg cctgctcatg acgaactaat acatgatcaa
    36001 taccacccac ggtatgcaat gcatcataaa tatcaaggac tgcgcctccg ggataaccga
    36061 atacttgttt aacgccctga tcgataagcg atcggacgac catctcggct ccagacaaca
    36121 tctccatggc ctgcctcact gtttgacgga aaaaatgtgt aaaacacatt aaccgtccga
    36181 tcttgcttaa gcaattggca gccagagaca taaacacggg gtaataagaa taaacaggag
    36241 gaaaggtgaa aagaaacgat aaaatcctcc attacagagg attaaattga taat
//
LOCUS       DAPHBJ010000023        35736 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.023, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000023 DAPHBJ010000000
VERSION     DAPHBJ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.023"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..105)
                     /locus_tag="RHZ76_001066"
     CDS             complement(<1..105)
                     /locus_tag="RHZ76_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 domain-containing protein"
                     /protein_id="HDV2811742.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSENALIIKMND"
     gene            complement(232..963)
                     /gene="rlmB"
                     /locus_tag="RHZ76_001067"
     CDS             complement(232..963)
                     /gene="rlmB"
                     /locus_tag="RHZ76_001067"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDV2811743.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1143..3584)
                     /gene="rnr"
                     /locus_tag="RHZ76_001068"
     CDS             complement(1143..3584)
                     /gene="rnr"
                     /locus_tag="RHZ76_001068"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDV2811744.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPSTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3623..4048)
                     /gene="nsrR"
                     /locus_tag="RHZ76_001069"
     CDS             complement(3623..4048)
                     /gene="nsrR"
                     /locus_tag="RHZ76_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HDV2811745.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4253..5551)
                     /gene="purA"
                     /locus_tag="RHZ76_001070"
     CDS             complement(4253..5551)
                     /gene="purA"
                     /locus_tag="RHZ76_001070"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDV2811746.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5655..5852)
                     /locus_tag="RHZ76_001071"
     CDS             complement(5655..5852)
                     /locus_tag="RHZ76_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HDV2811747.1"
                     /translation="MNSTIWLALALVLVLEGLGPVLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5934..6938)
                     /gene="hflC"
                     /locus_tag="RHZ76_001072"
     CDS             complement(5934..6938)
                     /gene="hflC"
                     /locus_tag="RHZ76_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HDV2811748.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6941..8200)
                     /gene="hflK"
                     /locus_tag="RHZ76_001073"
     CDS             complement(6941..8200)
                     /gene="hflK"
                     /locus_tag="RHZ76_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HDV2811749.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8286..9566)
                     /gene="hflX"
                     /locus_tag="RHZ76_001074"
     CDS             complement(8286..9566)
                     /gene="hflX"
                     /locus_tag="RHZ76_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDV2811750.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9642..9950)
                     /gene="hfq"
                     /locus_tag="RHZ76_001075"
     CDS             complement(9642..9950)
                     /gene="hfq"
                     /locus_tag="RHZ76_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDV2811751.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(10036..10986)
                     /gene="miaA"
                     /locus_tag="RHZ76_001076"
     CDS             complement(10036..10986)
                     /gene="miaA"
                     /locus_tag="RHZ76_001076"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDV2811752.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10979..12826)
                     /gene="mutL"
                     /locus_tag="RHZ76_001077"
     CDS             complement(10979..12826)
                     /gene="mutL"
                     /locus_tag="RHZ76_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDV2811753.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12836..14173)
                     /gene="amiB"
                     /locus_tag="RHZ76_001078"
     CDS             complement(12836..14173)
                     /gene="amiB"
                     /locus_tag="RHZ76_001078"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HDV2811754.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14192..14653)
                     /gene="tsaE"
                     /locus_tag="RHZ76_001079"
     CDS             complement(14192..14653)
                     /gene="tsaE"
                     /locus_tag="RHZ76_001079"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDV2811755.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14625..16172)
                     /gene="nnr"
                     /locus_tag="RHZ76_001080"
     CDS             complement(14625..16172)
                     /gene="nnr"
                     /locus_tag="RHZ76_001080"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDV2811756.1"
                     /translation="MTDHTMKKNSVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLRQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16171..17310
                     /gene="queG"
                     /locus_tag="RHZ76_001081"
     CDS             16171..17310
                     /gene="queG"
                     /locus_tag="RHZ76_001081"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDV2811757.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17293..17346)
                     /locus_tag="RHZ76_001082"
     CDS             complement(17293..17346)
                     /locus_tag="RHZ76_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2811758.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17580..17655)
                     /locus_tag="RHZ76_001083"
     tRNA            complement(17580..17655)
                     /locus_tag="RHZ76_001083"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17620..17622),aa:Gly,seq:gcc)
     gene            complement(17692..17767)
                     /locus_tag="RHZ76_001084"
     tRNA            complement(17692..17767)
                     /locus_tag="RHZ76_001084"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17732..17734),aa:Gly,seq:gcc)
     gene            complement(17978..18523)
                     /gene="orn"
                     /locus_tag="RHZ76_001085"
     CDS             complement(17978..18523)
                     /gene="orn"
                     /locus_tag="RHZ76_001085"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDV2811759.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18618..19670
                     /gene="rsgA"
                     /locus_tag="RHZ76_001086"
     CDS             18618..19670
                     /gene="rsgA"
                     /locus_tag="RHZ76_001086"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDV2811760.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDISDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19767..20735
                     /gene="psd"
                     /locus_tag="RHZ76_001087"
     CDS             19767..20735
                     /gene="psd"
                     /locus_tag="RHZ76_001087"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDV2811761.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20757..24080
                     /gene="mscM"
                     /locus_tag="RHZ76_001088"
     CDS             20757..24080
                     /gene="mscM"
                     /locus_tag="RHZ76_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HDV2811762.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24109..24423)
                     /locus_tag="RHZ76_001089"
     CDS             complement(24109..24423)
                     /locus_tag="RHZ76_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HDV2811763.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(24420..24734)
                     /locus_tag="RHZ76_001090"
     CDS             complement(24420..24734)
                     /locus_tag="RHZ76_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2811764.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            complement(24786..26288)
                     /gene="yjeM"
                     /locus_tag="RHZ76_001091"
     CDS             complement(24786..26288)
                     /gene="yjeM"
                     /locus_tag="RHZ76_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HDV2811765.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGMTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHLSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            complement(26507..27484)
                     /gene="epmA"
                     /locus_tag="RHZ76_001092"
     CDS             complement(26507..27484)
                     /gene="epmA"
                     /locus_tag="RHZ76_001092"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDV2811766.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            27809..29617
                     /gene="frdA"
                     /locus_tag="RHZ76_001093"
     CDS             27809..29617
                     /gene="frdA"
                     /locus_tag="RHZ76_001093"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDV2811767.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            29610..30344
                     /gene="frdB"
                     /locus_tag="RHZ76_001094"
     CDS             29610..30344
                     /gene="frdB"
                     /locus_tag="RHZ76_001094"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDV2811768.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            30355..30750
                     /gene="frdC"
                     /locus_tag="RHZ76_001095"
     CDS             30355..30750
                     /gene="frdC"
                     /locus_tag="RHZ76_001095"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDV2811769.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            30761..31120
                     /gene="frdD"
                     /locus_tag="RHZ76_001096"
     CDS             30761..31120
                     /gene="frdD"
                     /locus_tag="RHZ76_001096"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDV2811770.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            31183..32316
                     /locus_tag="RHZ76_001097"
     CDS             31183..32316
                     /locus_tag="RHZ76_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDV2811771.1"
                     /translation="MFKTTLCALLITASCFTFAAPQKINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWIRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVTAAWQILNALQ"
     gene            32405..32938
                     /gene="blc"
                     /locus_tag="RHZ76_001098"
     CDS             32405..32938
                     /gene="blc"
                     /locus_tag="RHZ76_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDV2811772.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(32935..33252)
                     /gene="sugE"
                     /locus_tag="RHZ76_001099"
     CDS             complement(32935..33252)
                     /gene="sugE"
                     /locus_tag="RHZ76_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDV2811773.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(33434..33580)
                     /gene="ecnB"
                     /locus_tag="RHZ76_001100"
     CDS             complement(33434..33580)
                     /gene="ecnB"
                     /locus_tag="RHZ76_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDV2811774.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(33691..33816)
                     /gene="ecnA"
                     /locus_tag="RHZ76_001101"
     CDS             complement(33691..33816)
                     /gene="ecnA"
                     /locus_tag="RHZ76_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDV2811775.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(33868..34434)
                     /gene="efp"
                     /locus_tag="RHZ76_001102"
     CDS             complement(33868..34434)
                     /gene="efp"
                     /locus_tag="RHZ76_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDV2811776.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            34476..35504
                     /gene="epmB"
                     /locus_tag="RHZ76_001103"
     CDS             34476..35504
                     /gene="epmB"
                     /locus_tag="RHZ76_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDV2811777.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         8526 a         9785 c         9069 g         8356 t
ORIGIN      
        1 atcattcatt ttaataatta atgcgttctc gctatttgtt acatcaatat tttgttcagg
       61 tacagtttgc agcgccgtcg ctagcgccaa cggattccat gtcatatatt attccatata
      121 gattaagttt aaatattaat aaaatgaata tttgcaatac gtaattatct taccagctat
      181 agacaaaaaa aaaccatcca aatctggatg gcttttcata attctgagaa attagctgcg
      241 ctggcgcacc gcttcaaata agcaaattcc ggtcgcaacc gaaacgttca gggaagaaac
      301 gcttccagcc atcgggatgc tgatcaactc atcgcaatgt tcacgagtca ggcgacgcat
      361 accttcacct tccgcaccca tcaccagcgc caggcgtccg gtcattttgc tctgatagag
      421 tgtatgatcc gcctcgcctg ccgtaccgac gatccagata ttctcttcct gcaacatacg
      481 catggtgcgc gcaaggttag tcacccgaat cagcggaacg ctttctgccg cgccgcaggc
      541 tacttttttc gccgtagcgt tgagctgtgc ggagcgatct ttcggcacaa tcaccgcatg
      601 aacgcctgcg gcgtccgcac tacgcaggca cgcgccgagg ttgtgcggat cggttacgcc
      661 gtcgaggatc agcaggaacg gttgatcgag cgaagcgatc agatccggca gatcgttttc
      721 ctgatactga cgtcctggct tcacgcgggc gataatgccc tgatgcacgg caccgtcgct
      781 tttctcgtcg agatattggc ggtttgccaa ctggataacc acgccctgag actcaagggc
      841 gtgaatcagc ggtaacagac gtttatcttc acggcctttt aaaataaaga cttcctgaaa
      901 acgttcaggg gcgcgctcca gcagggcctg cactgcgtgg atgccgtaaa tcatttcgct
      961 cattaatgta ctcgttgttt acgttttgcc tgatgcacta cgtttatccg gcctacatga
     1021 tctctgcaat atattgaatt tgcgtgatta tgtaggccgg ataaggcgtt cacgccgcat
     1081 ccggcatgaa caaagcgcat tttgtcagca atctaaccct cttcttttaa agagggtatt
     1141 gatcactctg ccactttttt cttcgccgca cgcttcgctt tggtcgctgc agctattttc
     1201 tgcgttttcg ccgatggctt tttcgctttt ctcgcgtctt tcttcgccgc tttcggcttc
     1261 gtttttttct caccgcggaa ggcgctgtct ggctcaaagt ttaccttttt accgacctga
     1321 cgacgcttgc cgcctttttt acctgcatcg ccttttttcg ctttctcgcg cgccgtttta
     1381 ccgacgttgc gcggtgcgcg ttcgctggag atcaggctaa agtcgatttt gcgctcgtcc
     1441 atattaaccg cttcgacgcg aacttccacg cgatcgccca ggcgataagt ctggccgctg
     1501 gattccccca tcaggcgttg ccctacctgg tcaaagcgat agtagtcatt gtccagcgaa
     1561 gagacatgga ccagaccatc aatgaacaag tcgtccagac ggacgaagaa gccaaagcca
     1621 gtgacgctgg aaattacgcc tttaaagacg ttacctacct ggtcgagcat gaagtcacac
     1681 ttcagccagt cagccacatc gcgcgttgct tcgtcggcac gacgttccgc catcgaacag
     1741 tgctgaccca gttgcagcat ctcttccatc gaataatggt agccgccggt ttcagtggtg
     1801 ttgccctgat gcccctgctc tttcgccagc agatatttaa tggcgcggtg cagcgtcagg
     1861 tctggataac gacgaatcgg cgaagtaaag tgcgcatagg actgcaatgc caggccaaag
     1921 tgaccacggt tttctggatc gtaaatcgcc tgtttcatcg agcgcagcag catggtttgc
     1981 agcatttctg catcaggacg atcggcaacc gactccagca gctccgcgta gtcacgcggt
     2041 tccggcttgt taccgcccgg cagttccagc cccagctccg ccagcactga acggaaagag
     2101 gtaatcgctt cggtgctcgg cttgtcgtga atacggaaca gtgccggttc tttcgctttc
     2161 tcaacgaaac gcgccgccga gatattcgcc agaatcatgc actcttcaat taatttgtgc
     2221 gcgtcgttac gctgggtctg ttcgatacgt tcaatacggc gttcagcgtt gaaaatgaac
     2281 ttcgcttctt cgctctcaaa tgagatccca ccgcgttctt cacgggcttt atccagcact
     2341 ttatagaggt tatgcaactc ttcgagatgc ttaaccagcg gggcgtactg ctcgcgcaga
     2401 tcctgatcgc cctgcagaat atgccagact ttggtgtagg tcagacgcgc gtgagagctc
     2461 atcaccgctt cgtagaattt gtagcccgtc aggcggcctt tcgacgaaac cgtcatctcg
     2521 cacaccatac acaggcggtc tacctgcggg ttgagcgaac acaggccgtt agagagcact
     2581 tccggcagca tcgggataac ctgcgaaggg aagtacaccg acgtgccacg gttacgcgct
     2641 tctctgtcca gcggcgttga cggacgcaca tagtagctga cgtcggcaat cgcgacccat
     2701 aaacgccagc cgccgccgcg ttttttctcg cagtaaactg catcgtcaaa gtcacgggcg
     2761 tcttcgccat caatggtgac cagcggtaaa tcgcgcagat caacacggcc cgcttttgct
     2821 tcttccggca cttcttcttt cagcccggca acctgttgct caacagcctg cggccagatg
     2881 tacggaattt catgggtacg cagagcgata tcaaccgcca tgccggtgcc catattgtcg
     2941 cccagcactt cgacgatttt acccaccgct ttggtgcggc gagtcggacg ctgagtcagt
     3001 tcgactacga ccacaaagcc catccgcgcg cccatgatct gatcgggcgg gattaagata
     3061 tcgaagctca gacggctgtc gtcaggaacc acaaagccga cgcccgcttc ggtaaagtag
     3121 cgaccaacaa tctggctggt ttttggcacc agtacgcgga caatacgcgc ttcacgacga
     3181 cctttacggt cagcacccag cggctgagcc agcacctgat cgccatgaat gcaggttttc
     3241 atctgctcgc tggagagata caaatcatct ttacgccctt caacccgcag aaagccgtag
     3301 ccatcacggt ggccaataac ggtacctttc accaggtcga ggcgttccgg cagcgcatag
     3361 cactgacgac gagtgaagac cagttgacca tcgcgctcca tcgcgcgcag gcggcgacgc
     3421 aggccttcaa gctgctcttc gccttcaatg tgcagttcta ccgccagctc atcacggctg
     3481 gccggttttt cacgtttggt taaatgttcg aggataaatt cccgactagg gatgggattc
     3541 gcgtattttt cagcttcgcg ttcctggaaa ggatcttgtg acatctcggt tcctccgttg
     3601 tcatctctga tgaagatttt cgtcactcca ccagcaataa tttataaagc ggttgattct
     3661 cttcaaccaa atcggcaagc gtgtagttat ccagttccgt aagaaaactt tgcacggcct
     3721 tagaaagtgc ctgtttcaac ctacaggcag gtgtaatgtg gcaaaactca ctgctgcaat
     3781 tcaccagcga taagggctcc agctcgcgca ccacatcacc aatacgtatc gcactcgccg
     3841 gtttacccag gcgaatgccg ccattttttc cacgaacagc agtcacgtag ccggcacgac
     3901 taagttgatt gattattttg accatatgat tacgggagac gccgtagacg tcagtcactt
     3961 cagaaatact ggtcatccgc ccttctggca atgacgccat gtagatcagc gcacgtaatc
     4021 cgtaatcagt gaaactcgtt aactgcacat caacctcaaa agggaaatcg ggaaaaaata
     4081 catttatatt gatgataaac cagccacaag ctgtgtcgct aatttatttc agttcgggga
     4141 aggaaaaaag cgaggattta acactgtgtc ggatagcgta atggcttacc cgacacagca
     4201 atgtatcaca ctgttcgccc ggcaggcaaa atatctgcca ggcgtaccag aattacgcgt
     4261 cgaacgggtc gcgcagaatc atggtttcag tacgatccgg accggtagag atgatatcga
     4321 tcggcacacc agtcagctct tcaatacgct tgatatagtt cagcgccgcc tgcggcaggc
     4381 cgctacgatc tttcacgccg aaggtggatt cagaccagcc cggcatggtt tcgtaaatcg
     4441 gctctacacc tttccagtcg tcagctgcca gcggagtggt agtcacttcg cgaccatccg
     4501 gcatacggta agccacgcag agtttaacct ctttcaggcc atccagaacg tccagtttag
     4561 tcaggcagaa gccagacagg gagttcagct gtaccgcacg acgaacggca acggtgtcca
     4621 gccagccggt acgacgacga cgccccgtag ttgcgccgaa ttcgttaccc tgcttgcaga
     4681 ggaactcgcc agtttcatca aacagttcgg tcgggaacgg acctgcacct acacgagtgg
     4741 agtaagcttt gaggataccc agaacgtaat caacataacg cgggcccagg ccggaaccgg
     4801 tcgccacgcc accagcagtg gtgttggaag aagttacgta cggataagta ccgtggtcga
     4861 tatccagcag cgtaccctgc gcaccttcaa acatgacgaa atcgccacgc tgacgcgcct
     4921 ggtcgagcag gtcagaaacg tcaaccacca tagaagtcag gatgtcggca acagccatcg
     4981 tatcatccag aactttctgg taatcaaccg cttcagcttt gtagtagtta accaactgga
     5041 agttgtgata ttccatcact tctttcagtt tttcagcgaa ggtttctttg tcgaaaaggt
     5101 cgccaacacg cagaccgcga cgtgctactt tatcttcata agcaggcccg ataccacgac
     5161 cggtggtgcc gatcgctttc gcgccacgcg ctttctcacg cgcgttatcc agcgcaacgt
     5221 gataatcaag gatcagcgga catgcttcag acagcagcag acgctcacga acggggatgc
     5281 cacggtcttc cagttctttc atctctttca tcagcgcggc cggagacagc acaacaccgt
     5341 taccgatgat gctggttaca ttctcgcgga gaatacctga tggaataaga tggagaacgg
     5401 ttttttcacc gttgattacg agagtatggc ctgcgttgtg accgccctgg tagcgtacaa
     5461 catatttagc ccgttcagtc agaagatcga cgatcttacc tttaccttcg tcaccccatt
     5521 gggtgcccag tacgacgacg ttgttaccca tttttcaaaa tcaccgtttg cttaaaaatg
     5581 gattctacca tcgctttttc agagttacag cactttttgc actcaaaaat gacctcgatc
     5641 agtctgtttt ttgttcagcc aatcgttttc ctcaacatgt agtagaccac aacgcccgca
     5701 accacaagtc caccgccaaa acgacgtaaa atattatcgg gcaaattggt catcgcagag
     5761 atcatcttct tccatgcctt cgggtaaagc accggcccta aaccttccag taccaaaacc
     5821 agggcaagcg ccagccagat tgtcgaattc attcattatc cttatagaaa aagaaaacca
     5881 ccgacatccc tgaggatgcg gtggctttat tgacctgtac cgcagtcgtt atattaacgc
     5941 gttgcggaag tcggcgtctt catgtagcgg aagaaatcgc tatccgggct catgaccatc
     6001 acgtcctgat tgccagagaa gctgttctca taagcacgca ggctacggat gaatgcgtag
     6061 aagtccggat ctttactgaa tgcatcagca aacagtttgg ctgcttcggc atcgccttca
     6121 ccacgcatga tgcggccctg acgctcagct tctgccagcg ttctggtcac ttcatagtcg
     6181 gcagtcgcgc gcagtttttc cgcttcttcc tgaccttgtg aacggtgacg acgcgctacc
     6241 gcttcacgct cggcgcgcat acggttgtag atcgcttcag acacttcggt cggcaggttg
     6301 atctgcttga tacgcacatc gacaacttca atacccagcg ccgccatact gttcgggttg
     6361 atgaccggaa ctttgccctt cgtctcagcc gttacgcgct ctgccgcttc ggcaatggcg
     6421 ttatctgccg ccggggtagt aacttcatct tctgtacccg cagaaccgga gttcagcgcg
     6481 tcacgtactt cgagggtcag acgaccacgg gaatcggtga cgatatcttt cacgtcgagg
     6541 cgaccaattt cagaacgcag acggtcagag aacttacgtt tcaacagcac ttccgcttgc
     6601 gaaatgtcgc caccacccgt tgccaggtag taacggctga aatcgctgat gcgccatttg
     6661 atgtaagagt cgacgatcag gtctttcttc tctttggtca caaagcggtc ggcctggttg
     6721 tccatggtct gaatacgtgc gtcgagcatt ttcaccgttt caatgaacgg tatcttgaaa
     6781 tgcagacccg gctcataaac cagaggtttg ttgtcatcgt cacgcagtac cttaccaaaa
     6841 cgcagcgtaa taccgcgctc accttctttg acgacaaaga cagacatgta aagcactacc
     6901 agcacgatga tgataatcgc gataactgac ttacgcatcg ttattccccc tgacgctggt
     6961 agtcgttacg ctgcgcgttg gcgcggcgtt ggtccataat atcgccctga ctggtggacg
     7021 acgtgttgct tgctccactg gttgtggagg aagaggctgg cggcagacgc agcagattgc
     7081 tggcaccgtt atcgctcttc gccgcagggg cgttaccacc tttcagcatc tggtctaacg
     7141 gcagaaccat caggttgcca cctttatcgt taaccagcac tttgcgggtg ttacccaaca
     7201 ctttttccat cgtctcgata tacagacgct cgcgagtaat ttccggcgcg gctttatatt
     7261 ccggcagaag tttagcaaag cgcgccactt caccctgagc ttccaggatg gtctgggcct
     7321 tgtacgcacg cgcctcttcg aggatacgtt gcgcctgacc gttcgcacgc ggctgaactt
     7381 cgttggtata cgcttctgct tcacgaatgt attgctgttc gttttcacgc gcggcaatcg
     7441 catcgtcaaa cgccgctttt acttcttccg gcggacgagc agcctggaag ttgacgtcca
     7501 gcagcgtgat acccatgtca tacggacgaa tcgtctcttc cagttcgcgc tgagtatcgc
     7561 tacgaatcac ggtacgacct tccgtcagaa tgcggtccat ggtgtattta ccgataactc
     7621 cacgcagggc gctgtcggta gcctgacgca ggctgtcatc cgggctggtc acgctataca
     7681 gatatttttc cggattggtg acgcggtact gcacgttcat ctcaacgcgc actacgttct
     7741 cgtccgacgt cagcatcaca ccagaagcgg ccagttcacg cacggcttcc acgttcaccg
     7801 gtttgacttc gtcgataaac gtcggtttcc agttcagacc cggctcaacc agatggctga
     7861 atttaccaaa gcgtgttacc acgccgcgtt cggcttcttt aatggtatag aaaccactgg
     7921 ccgcccagat aatgacaatc gctgccgctg cgatggtaac gacacgaccg ccaagctgcg
     7981 ggcgcgggcc ttgcgatgaa ctgccaccgc cagatccggt gcctttaccg ccgcccagac
     8041 caccgagctt tttgctcagt ttgcggaaga tatcatctaa atcaggtggc ccttgatcgc
     8101 gaccgccttt gtttccattt ccctcagagt tgccgccagg tttgctgctt ccccacgggt
     8161 cgcggtcttg tccgttatta ccgggctgat tccacgccat gtttgtgctc catatttgtt
     8221 atgcggtgat ccctgttgtc tttcgacgcc agaggatatg actccacgct tcagacgcta
     8281 cgccgttaga tcaggtaatc gatcaacgcc ggttcttgtt tacagaggcg acgccagtca
     8341 acgatcggca tacgaacttg cagacttacg ctgccgtcct cctccatcca ctctttttct
     8401 attgcctgaa gctgataaaa acgacttctc agacgccctt cctgcggtgg cagacgcaat
     8461 gtatgctgcg ccacctcgcc ggaaagccgc tccgtcaaag cctgaaaaag ctgtggtatc
     8521 cccgctccgg tctgtgcgga aagccagaca cggatcggtt tgttctcttc gtcccgatca
     8581 atacgcggtt cgaaatcttc cagcatatcg atcttgttca tcaccagcag ggttgggatc
     8641 tcgtgagcgt cgatctcttc aagaaccgta ttcaccgctt cgatgttttc ttgtacacgc
     8701 acatccgccg catcaatgac gtgcagcagt aatgtggctt gccgcgtctc ttgtaacgtg
     8761 gctttaaatg ccgccaccag atcgtgcggc aggtggcgaa taaaccctac ggtatctgca
     8821 agtacggttt caccgacatc tgcaacgtca atacgccgca acgtcgggtc gagggtggca
     8881 aacaactggt ctgccgcgta gacccgcgct tcggtgatgc gattgaaaag ggtagattta
     8941 ccggcgttgg tatatcccac cagcgaaaca gtaggaacgt cggctttgat acgcgattgc
     9001 cgcccctgct cacgctgctt ttcaactctt tccaggcgcg actgtatctg cacgatgcga
     9061 ttacgcaaca aacgacggtc ggtttcgagc tgggtttcac ccggaccacg caaacctatc
     9121 ccgcctttct gtctttcaag gtgggtccag ccacgcacca ggcgcgtagc cagatggcgc
     9181 agctgcgcca gctcaacctg caacttaccc tcatgggtac gcgcacgttg ggcgaaaata
     9241 tctaaaataa ggccggtgcg gtcgataaca cgacactcgc acaaacgctc caggttacgc
     9301 tcttgcgccg ggctcagggc atggtcaaaa agaacgaccg aagcacccgt cgctttgaca
     9361 gcttccgcaa tttcaactgc tttaccttca cctacaaaat actttgggtg cggcgcttta
     9421 cggctaccgg taatcacctg caatgcttcg acaccggcgg aagagaccag agattcaaac
     9481 tcctggaggt cttccatatc tttgtcttgc gtaaaataga tgtgtaccag taccgcctgc
     9541 tcaccagcat cataacggtc aaacaagcgt ataaccctct aaatagatca gcggggaacg
     9601 caggatcgct ggctccccgt gtaaaaaaac agcccgaaac cttattcggt ttcttcgctg
     9661 tcctgttgcg cggaagtatt ctgcgcgctg ctaccatgat ggtagttact gctggtaccg
     9721 ccaccggcgt tgttactgtg atgagaaacc gggcgagacg ggacaacagt agaaatcgcg
     9781 tgcttgtaaa ccatctggct gaccgtgttt ttcaacagga tcacgaactg atcaaaagac
     9841 tcgatttgcc cttgcagctt aataccattc accaaataaa tagaaactgg aacacgttcc
     9901 cgacgcagtg cgttcaggaa cggatcttgt aaagattgcc ccttagccat tctctctttt
     9961 ccttatatgc ttatttgtac tttgaacctt tcgattctga aaaaattgcg cacgatacgt
    10021 ctcaattgta cacattcagc ctgcgatagc accaacaacc tgtaatactt cgtcacgcgc
    10081 ctgttctggt ttttcactgt caagccagtg aaccccttcc caaccacgca gccaggttat
    10141 ctgccgcttc gccaactgtc tcgtggcgca aacacctcga taaaccattt catcgtatga
    10201 gatttcgcct tcaaggtaag accacatctg gcgataaccc acgcaacgaa tggaaggcaa
    10261 gtccgtatgc aaatctcctc gggcaaaaag cgcccggact tctgcttcaa aacctgaagc
    10321 caacatctga tgaaaacgct gctcaatgcg ttgatggagc agttcacggc tcgccggggc
    10381 gatggcgaac tgatgcacct gatacggtag agcgtctcct gacgtttgcg tcagttccgt
    10441 taaagtttta cccgaaatga aaaaaacttc cagtgcccgg gaaagccttt gtggatcatt
    10501 tggatgaatc cttgctgccg caaccggatc tacctcctga agttgacgat gcaatgactc
    10561 ccaaccttgc tctgccgcct gttgctcaat tctggcccgt acttccgggt ctgccgacgg
    10621 tagcggcgac aacccttcca gcaatgcctt gaaatacaac atcgtaccgc ccactaacag
    10681 tgggatccgc cccgccgcgg tgatatcggc catttccgcc agcgcatcgc ggcgaaaatc
    10741 agcagccgag taagcctgcg acggatcgcg aatatccagc aatcggtgcg gcgcggcgag
    10801 taactcttca gcgttcggct tcgccgtccc gatatccatc cctttgtaaa taagggcaga
    10861 atcaacgctt atcaactcta ctggtaaaat tttacgcagc tcaatggcta acgccgtttt
    10921 accggaggcc gtcggcccca tcaaaaaaat cgccttaggc aggctcgcct tactgatatc
    10981 actcatcttt cagggctttt atcgccggat gtaaatcaac agattgtaac agaccacccg
    11041 gcggcgtttt cacaagttgc ggacataacc gttccacgtc cgccagcagg gttatggcct
    11101 gtgccattga ccactgcgca tgttcgctca tcagatttcg tgcaatccac tgcgcaatat
    11161 tgccaggttc gaatacggac tgcttcgcca ggtagcctat cagttcagga atcaagattt
    11221 gtaaattttg ttggcgtaag ggtaaaggca ctgccctgat ggtcacatgc tgtgcatctg
    11281 actggaaatc aatacccaat tccgccaggg cagactgcgc tttttctaat gccgattttt
    11341 cttcgccaga aacttttagc cgcaacggaa tcagcagcgg ctgggcgcaa acgggcgctt
    11401 cacccggcgt caattgtgcc tgacgcagcc aacgttctgc cactggcaag gataaaagtg
    11461 aaatgttgcc gtcgcgctcc agcaacgcac agtcggaatg gacgatagtc agtacccgac
    11521 caaaactctg actgttcgcc gcaagtgcag gttcctgcgg ttccggcgct tttaattttt
    11581 gcatcggcgc gggcgtttgc aaaagctggc gatacacttc accttgctgt ttctggtagc
    11641 ctggctgcgc attcggccag ggggcagccg gacgactgcc tgatgctggc gcaggagtgt
    11701 agcgcggagc taccggctca cgagctgccg gttctgcaaa gtgattgcgc cccgccgcca
    11761 cgcggttttc cggaatggaa cgcggtgcag gttggggttc atcgtccagc ggtagcggcg
    11821 tttccagttg ctgttgtagc acgctcagca cgccctgata gataaaatca tgcaccagac
    11881 gcgactgatg gaaacgcact tcgtgtttgg cggggtgcac gttgacgtcc acctgatgtg
    11941 ggtcgatctc cagatacaac acaaatgccg gttgctgatc ggcccccagt ttgtcttcgc
    12001 aggcctggcg gatcgcgtga ttgatcaggc gatcgcgcat catgcgaccg ttcacgtagc
    12061 aatactgaat ttctgccagt gcgggcgtgg tgtgatttgg atcggccacc cagccgcgta
    12121 gcgtgagatc gccgtgttgc cattcaatcg ccagcgcttg ttcaagaaaa gcggtgccgc
    12181 aaatcgcgcc taagcgccgt tctttttgcc cgccttccgg cactgcgcgg tactgacgca
    12241 caattttacc gttatgcgac aggttgatcg tgacgtcgaa acgcgccagc gcaatgcggc
    12301 ggatgatctc atcaatgtgg ttaaattcgg ttttctcggt gcgcaggaat ttgcgccgcg
    12361 ccggggtgtt gtagaacaga tccagcacct ccagcgtcgt ccccacagga tgcgccgccg
    12421 gttttaccgt cacgttcata tcgcgccctt cggcataggc ctgccaggct tcctgctgtt
    12481 ctgcggtgcg tgaagtgagc gtcaggcggg aaaccgaact gatactcgcc agcgcctcac
    12541 cgcgaaagcc caggctgata atggcttcga gatcgtccag agaggcgatt ttactggtgg
    12601 catgacgagc cagcgccagc gccagctcat cttttttgat accgcagccg ttatcacgaa
    12661 tgcggataag tttcgcccca ccgcgttcga tatcaatatc gatacgcgtc gcacctgcat
    12721 cgaggctgtt ttccactagt tctttgacta ccgacgcagg tcgctcgacc acctcacctg
    12781 cggcaatctg gttcgccagt tgtggcggta agacctgaat tggcatcaat cgtccttagt
    12841 ttggcagcgt gcgatctggc gtcgtcaccg tactggcagt ttgtgccgtt gcaccctgcg
    12901 gcgcagattg catcggatgc gcaaggaagt aattgcgcag gcctttgtaa atggcttctg
    12961 ccagctgttg ttggtaatcg tcgctcgcca gcaaacgttc ttcgctgttg ttgctgataa
    13021 aaccggtttc gaccagtact gatgggatat ccggcgagcg cagaacgcca aggctggcgt
    13081 gttctggtcg acgtttatgt atttcgccaa tgcgttgcaa ctgactgatc atactggtcg
    13141 ctacatcata ccctacccgc tgggaatgac cgaactgtaa atccagcacc gcctggctta
    13201 aataggggtc agactgactg ttcgccagca catcacccgc cccacccagc agctccgact
    13261 gtttctcgtg ctgctccagc cagctggcca tttcactgtt ggcgcgacgg ttagagagca
    13321 cccatacgga agcgccagtc gcactgcggt tcggtgcggc atcagcgtga atcgacacga
    13381 ggaaattggc gttttgctta cgtgccacat cactgcgccc catcaccgag ataaagtaat
    13441 ccccgtcacg ggttaaaacg cctttaaaca tcggatcgtc attgagcaaa gtacgcaatt
    13501 tacgcgcgat ggcgatggtg acatttttct cccgcgtacc accggggccg atagcgccag
    13561 ggtcctgacc gccgtgtccg gcatcaatag cgatgataat tttatcgcca gtgttagccg
    13621 tcgcgcgcgc tgccggacgc gttaccgtat tactgctgat aacacccgta gtgcggttac
    13681 tttccgtttt aaacggattg cgcgccggtt cgctgacgcg cggtgcgaca accgcaggcg
    13741 tttcaacgcg tttcgcaacc acgggcggcg gaggaggcgg tggcggcaca tcggcgttaa
    13801 tcgtaaagac gacagtgtaa ttgctgccat tctgccgctt caccgcttcg gttttaccgt
    13861 tttcggtaag atcgaccacc agccgtagcg tttgtgcatc tttaggcgtt ccagagcgaa
    13921 tcgccttcac cagattattg ccgctgaaca acaacggcag tccctgaatc acgcccgttt
    13981 gtttgatatc gagcgccacg gtgcgtttgc tttgatggct aaacgcataa tcaggatcgc
    14041 caataaaact caacgttatc cgcgcctgtt gattaccgtt agaaacctga atatcagaga
    14101 gcgtcgcggc acccaccggc gtgcacagca gcagcagcgt cgctaccaac caatttctga
    14161 tgcgatacat catcccgcca cctttcaaag gttaaccggc taaacgcgcc agcaacaatt
    14221 cacccgcaga ggaaaccgca ctcacgcgcg cctcacggcc ttgtgcctga taatcaatgt
    14281 gtatttcgac atccgggtca ggaagaacac ctgtaccttg ttgtggccac tccaccaggc
    14341 agatggcatc gttggcaaaa taatcgcgga tccccataaa ctccagctcc tcgggatcgg
    14401 caaggcggta caaatcaaag tgatagacca ttaagttgtc gagcgtatag ggttcgacca
    14461 gcgtataagt ggggcttttg acattaccct gatgacccag agcctgtaaa aagccccggc
    14521 taaaggtggt tttacctgcg cctaaatcgc catacagata gattacggtt gcgccatcgc
    14581 aggctttcgc tacccgctcg cccaggtcta atgttgcctg ctcatcaggg agcggaatta
    14641 ctcgattcat catggttttt atcagtcact tccgggttaa caatacgctg tagcgtggaa
    14701 aagagatcgg ttgccagcat cccgcgcgtt ccaaaacgcg ccgccagtac gtcagctgcc
    14761 gcaccgtgcg cgacacagcc tgcacaggct gcatcatacg gcgacagttt ttgcccaagc
    14821 aatgcgccaa taataccaga gagcacatcg cccatgccgc cgctcgccat gcctgcattt
    14881 ccggcatcaa taatgcctaa agcgtcagga tgggcggcga ccacggttcc ggcacctttc
    14941 agcaccgcta cgccgccata acgttgtacc agacgtttgg cgcaatgtaa gcggtcactt
    15001 tcaatttcag cgacggaaca gcctaacaac cgtgcggcct cgccaggatg cggcgtgatc
    15061 acgcgattgt gacgcttatc gggattgatt gccagcaggt tcaatgcatc ggcatcccac
    15121 aacatcggtt tgcgaaaatt ctcaactttt tgcagtgctt ttttccccca ctcttgctgg
    15181 cccagaccgg gaccaatcac caccacatcg gcccattcca ggctttcggt aagagagtcc
    15241 atcgtcagtt catgcaccat caattccggt cgtgcagtca gcagcggcgc aatgttttca
    15301 ctgcgggtca gtactcggac taaaccagca ccagcacgca gcgccgcttc ccccgtcata
    15361 cgaatagccc ccgccgtgcc gtgatcgcca ccgataatta ccagccgccc gtgatcgcct
    15421 ttatgcgaag tcgggcgacg cggttttagc cagtgagaaa gttgttctgc tgaaaaccgc
    15481 tgaattttcg tctcctgacc tgccagccaa ctatccagcc ccagtgagtc aaaatgcagt
    15541 tgtccggtaa catcccgcgc ttttccagtg agcaagcctg gtttcagcgc aataaaagtg
    15601 atggtgtgat cggcgttgat caccgcgcct ggcgtagcgc cagtttcagc cagcaggccg
    15661 gaagggatat caaccgccac aatcggcgca ggatgggaat tagcgtggtc gattaactgg
    15721 ctaatggatt cgcggggcgc ttgccgcaaa ccggtaccga gcagcgcatc aacaatcaga
    15781 tctaccgatt cgggccagac aatattcgaa gcatggatct cgccacccgc gtttaaccat
    15841 gcttcgcgtg ccagcgcggc ctcttccggc aacggtttgt cgctctcctg ggccaacaac
    15901 gtgacctcaa tgccgaccgc tttggccagt cgcgcgacca cgtagccatc gccgccgtta
    15961 ttaccatgac cgcacagcac cagccagtgg cgggcgtcag gatacgccga acgacacacc
    16021 tggaatgcgg cctcgccagc gcgaagcatc agctcataga gtgtgagccc cagcacatct
    16081 gccgcctcgc gttctccgcg gcggatatcg tcggcgtacc agacggtgtg tggtatactt
    16141 acggagtttt tcttcattgt atggtccgtc atgtcagagc ccctcgatct caatcagtta
    16201 gcgcaaaaaa ttaaacagtg ggggctggaa ctgggctttc agcaggtagg tattaccgat
    16261 accgatctca gcgagtccga gcccaaactg caagcatggc tggacaaaca ataccacggc
    16321 gaaatggact ggatggcacg tcacggtatg ctgcgcgctc gccctcatga gttattgccc
    16381 ggtacgctgc gcgtgatcag cgtgcggatg aattaccttc ctgctaacgc cgcatttgcc
    16441 agcacgctga aaaaccccaa actcggctat gttagccgtt atgcgctggg ccgtgactat
    16501 cacaaacttc tgcgcaaccg actcaaaaag ctgggcgaga tgattcagca acattgtgtt
    16561 tcgctgaatt ttagaccgtt tgtcgattct gcgcctattc tcgagcgccc gttagctgaa
    16621 aaagctgggc tcggctggac aggtaagcac tcacttatcc tcaatcgcga ggccggttcg
    16681 ttcttctttt taggcgaatt gctggtcgat attccgctgc ccgtggatca accagtcgag
    16741 gaaggatgcg gcaaatgcgt ggcctgtatg acgatttgcc cgaccggtgc catcgtcgag
    16801 ccatataccg tcgatgctcg ccgctgtatc tcttatctca ccatcgaact tgaaggggcg
    16861 atcccggaag agttgcgacc gttaatggga aaccgtattt acggttgcga tgactgccag
    16921 cttatctgcc cgtggaatcg ctattcacaa ctcaccacag aagaggattt cagcccgcgt
    16981 aagccgctac acgcaccgga actcattgag ttattcgcct ggagcgaaga gaagttttta
    17041 aaagtcacgg aaggatcggc gattcgtcgt attggtcacc tgcgttggct gcgtaatatc
    17101 gccgtagcat taggcaatgc cccttgggat gaaacgattt tgacagcgct ggaaagtcgt
    17161 aaaggtgagc acccacttct cgatgagcac atagcgtggg cgattgcgca gcaaatagag
    17221 agacgaaatg cgtgcatagt cgaagtgcaa ttgccgaaaa aacagcgtct ggttcgggtg
    17281 attgaaaaag ggttaccgcg tgacgcctga ttcatccaca gcttgtgaat aaaaataaaa
    17341 acgcattgca attcaagacg aataaaatcg tcaagtgatc gactcgacaa aacggaatct
    17401 taatttttaa cttatctatc aataggttaa aaaaatacta ttcaccgtgc gaagtttctt
    17461 tgaaattaag attacagcct gaggctgtgg ataactctgt ttacaagagt ttgtcagaga
    17521 caacaaaaac cagcaataac gcatcgcgtc gctgtggata ttttattgag agaagaattt
    17581 ggagcgggaa acgagactcg aactcgcgac cccgaccttg gcaaggtcgt gctctaccaa
    17641 ctgagctatt cccgcttggg tggtctgtgc tttgcagcac ttttcaaatt ttggagcggg
    17701 aaacgagact cgaactcgcg accccgacct tggcaaggtc gtgctctacc aactgagcta
    17761 ttcccgcgta ctacttaatt ttgctttcgt aattttcgca tttctgcgtc gttacgggag
    17821 gcgcattata cgagaaatct ttccctctgc aaccccccaa aaaagttttt tttgcgtttt
    17881 tatctcaagt gctgaaatta tcagcaagac gttcaattta gcgtcaaaat cacgccgccg
    17941 caagtggatt ccggcgctta cgtgaccagg aaaaatttta cagcttgata aaatgctcgc
    18001 ggtagtaagc cagctccgcc accgattcac ggatatcatc catcgcctga tgcgtcccct
    18061 gcttggtaaa accatccaga atttccggct tccagcggcg cgccagctct ttcagggtgc
    18121 tgacatcgag ataacggtag tggaagtagg cttccagctc cggcatgtat ttaaacagga
    18181 aacgacggtc ctgaccgatg ctgttaccgc aaatcggcga ttttcccgca ggcacccact
    18241 gttttaaaaa ttcgagcgtt gccagttcag cttcccgatc gcccatcgtg ctcgctttca
    18301 cgcgctctac cagcccgctg gcggtatggg tgcgcacgtt ccagtcatcc atcagcgcca
    18361 gctgttcatc agactggtgt actgcaatgg tcggcccttc tgccagaata ttcaggttgg
    18421 catcggtcac cagcgtggca atctcaataa tgcgatcgcg ctcgggatcc agaccggtca
    18481 tctcaagatc gatccaaatc aggttgtttt cattggcact catgctattt tccacccatt
    18541 cacagtgact atattgatcc acaattgcgt gtatcataga tgttttgccc atcaggggcg
    18601 accaggagtc tgtacgattg agtaaaaata aactctccaa aggccagcag cgccgcgtga
    18661 acgccaatca ccagcgtcgt cttaaaacgt ctaaggagaa gcccgactac gacgacaatc
    18721 tgtttggcga gcctgatgaa ggtatcgtca tcagccgctt tggtatgcac gctgatgtgg
    18781 aatccgccga tggcgacgtt caccgctgca atattcgccg taccatccgt tcgctggtaa
    18841 ccggcgaccg cgtagtctgg cgtccgggta aaccggcggc ggaaggcgta aatgtcaaag
    18901 ggatcgtgga agcggtgcat gagcgtacct cggtgttgac gcgtccggat ttctacgacg
    18961 gcgtaaaacc tattgccgcc aacatcgacc agattgtcat tgtctccgcc attttgccgg
    19021 agctgtcgct caatattatc gaccgttacc tggtggcctg cgaaaccttg cagattgagc
    19081 cgattattgt gctcaacaag atagacctgc tggacgacga aggcatggcg ttcgtcaacg
    19141 agcagatgga tatctaccgc aatatcggtt atcgcgtatt gatggtttcc agccatactc
    19201 aggatgggct aaaaccgctg gaagaggcgt tgaccgggcg catcagcatt tttgccgggc
    19261 agtctggcgt cggcaaatcc agcctgctga atgcgttgct ggggctgcaa aaagagatcc
    19321 tgaccaacga tatctctgac aactcggggc tcggtcagca caccacgacc gccgctcggc
    19381 tgtatcactt cccgcacggc ggtgatgtga ttgactcccc aggagtgcgt gagttcggcc
    19441 tctggcacct ggagccggaa caaatcactc agggctttgt cgaattccat gactatttag
    19501 gtctgtgtaa atatcgcgat tgcaaacacg ataccgatcc gggctgcgct atccgggaag
    19561 cggttgagga agggaaaatc gcggaaaccc gtttcgaaaa ctatcaccgt attctggaaa
    19621 gcatggcgca ggtaaaaacg cgtaaaaact tttctgatac ggatgactga caattaagct
    19681 aaccgtcgtt agaatcgtcc cctttttttc aggatccggc atgtaggccg gatcaggaac
    19741 gacaaaacaa tggcctggag gctaccttgt taaattcatt taaactttcg ctacagtaca
    19801 ttctgccgaa actatggctt actcgcctgg cgggttgggg cgcaagcaag cgggcaggat
    19861 ggctgacaaa actggttatc gatctgttcg ttaaatacta caaggtcgac atgaaagagg
    19921 cgcaaaagcc ggacaccgcc agctaccgca cctttaacga attctttgtc cgtccgctgc
    19981 gtgacgaagt acgcccaatc gataccgatc cgaatgtact ggtcatgcct gccgatggcg
    20041 ttatcagcca gttgggtaaa atcgaagaag ataaaatcct gcaagccaaa ggccacaact
    20101 acagcctcga agccctgctg gcaggcaact atctgatggc ggacctgttc cgcaacggta
    20161 cgtttgtgac cacttacctc tccccgcgtg actaccaccg cgtacacatg ccgtgcaacg
    20221 gtattctgcg tgagatgatc tacgtgccgg gcgatctctt ctccgttaac catctcacgg
    20281 ctcagaacgt gccgaatctg tttgcccgta acgaacgcgt gatttgcctt ttcgataccg
    20341 aatttggccc aatggcgcag attctggtcg gagcgacgat tgttggcagc attgagacgg
    20401 tctgggcggg caccattacg ccgccgcgcg aaggtatcat caagcgctgg acctggcctg
    20461 ccggggaaaa cgacggttct gtggcactgc tgaaaggcca ggaaatgggt cgctttaaac
    20521 tcggttccac cgttatcaac ctgtttgcac cgggtaaagt gaatctggtt gagcaactgg
    20581 aaagcctgtc tgtcacgaaa attggccagc cgctggcagt atctaccgaa acctttgtta
    20641 cgccagacgc tgaacctgcc ccgcttcctg ctgaagagat cgaagcagaa cacgacgcca
    20701 gcccattggt tgacgacaaa aaagaccagg tctaatccat caaaggaaac gctgacgtgc
    20761 gcctgattat cacttttctg atggcctggt gcctcagttg gggggcgtac gccgcgacgg
    20821 cccccgatag caaacaaatc actcaggaac tggagcaggc aaaagcggcg aaacccgcac
    20881 agccggaagt cgtagaggcg ctccagtctg ccttaaatgc gcttgaggaa cgaaaaggtt
    20941 cccttgagcg catcaaacaa tatcagcaag ttatcgataa ttatccgaaa ctctccgcta
    21001 ctctgcgcgc acaattaaac aacatgcgtg acgagccgcg cagcgtgtcg ccgggaatgt
    21061 ctaccgacgc gctgaatcag gaaattctcc aggtcagcag ccagttgctg gataaaagcc
    21121 gtcaggccca gcaagagcag gagcgcgccc gcgagattgc cgattcgctg aatcaactgc
    21181 cgcaacagca aaccgacgcc cgccgtcagt taaatgagat cgagcgccgc ctgggaacgc
    21241 ttaccggcaa tactccgctc aatcaggcac aaaatttcgc gttgcagtct gactctgcac
    21301 gtcttaaggc gctcgttgat gaactggagc tggcgcagct gtctgccaat aaccgccagg
    21361 aattagcgcg cttacgctca gagctggcgg aaaaagagag ccagcaactg gatgcgtatt
    21421 tgcaggcctt gcgtaatcaa ttaaacagcc aacgtcagct tgaggcggag cgggcgctgg
    21481 aaagtaccga attgctggca gaaaacagcg ccgatttgcc gaaagatatc gtcgcgcaat
    21541 tcaaaattaa ccgcgaacta tcggcggctt tgaatcaaca ggcgcagcgg atggatctcg
    21601 ttgcctcgca acagcgtcag gctgccagcc agacgttaca ggtccggcag gcgttgaata
    21661 cgctgcgtga acagtcgcaa tggctgggat cgtccaatct gctcggcgaa gcgctgcggg
    21721 cgcaggtggc acggctgccg gaaatgccga aaccacaaca gcttgatacc gaaatggcgc
    21781 agttgcgtgt gcaacggtta cgttatgagg atctgcttaa taaacagccg ctgctacggc
    21841 aaattcatca ggccgacggt cagccgctga ctgccgagca aaaccgtatt ctggaagcac
    21901 aactgcgcac tcagcgtgag ttgctgaact cattgttgca gggtggcgac acgctactat
    21961 tggaactgac caagctgaaa gtctccaacg ggcaactgga ggatgcgctg aaagaggtga
    22021 acgaagcaac gcaccgctat ctgttctgga cctctgacgt gcgcccgatg accatcgcct
    22081 ggccgctgga aatcgcccag gatctgcgtc gtctcatttc gctggacacc ttcagtcagt
    22141 tgggcaaagc cagtgtgatg atgctgacca gcaaagagac aattttgccg ctgtttggcg
    22201 cgttgattct ggtcggttgc agtatttact cgcgccgcta tttcacccgt tttcttgaac
    22261 gttcggcggc gaaagttggc aaagtgactc aggatcactt ctggctgacg ttgcgcactc
    22321 ttttctggtc gattctcgtc gcgtcaccgt taccggtgct gtggatgacg ctgggttacg
    22381 gcttgcgcga ggcgtggcct tatccgctgg cggtcgcgat tggcgatggt gtaacggcca
    22441 ccgtgccgct gctgtgggta gtgatgattt gcgccacctt tgcccgcccg aacggcttgt
    22501 ttatcgctca ttttggctgg ccgcgcgaac gtgtttcccg tgggatgcgc tactacctga
    22561 tgagcatcgg gcttattgtg ccgctgatta tggcgctgat gatgttcgat aacctcgacg
    22621 accgtgaatt ctccggttcg ctgggacggc tttgctttat cctcatttgc ggtgcgctgg
    22681 cggtggtcac cctcagcctg aaaaaggccg ggattccgct gtatctcaac aaagagggca
    22741 gcggcgacaa cattaccaac catatgctgt ggaacatgat gattggcgcg ccgttggttg
    22801 ccattctggc gtcggcggtg ggttatctgg caacggcaca ggcgctgtta gcgaggcttg
    22861 aaacctcggt tgccatctgg ttcctgctac tggtggttta tcacgttatc cgccgctgga
    22921 tgctgatcca gcgccgcagg ctggcgtttg atcgggcgaa gcatcgccgg gcagagatgt
    22981 tagcgcaacg tgcgcgtggc gaagaggaag cgcatcatca cagtagcccg gaaggagcaa
    23041 ttgaagtcga tgaaagcgaa gtcgatctcg atgccatcag tgcgcaatcc ttgcggctgg
    23101 tgcgctcaat tttgatgttg atcgccctgc tttctgtcat tgtgctgtgg tcagaaatcc
    23161 attccgcttt cggcttcctc gaaaatattt cgctgtggga tgtcacctcc acggtacagg
    23221 gcgtagaaag tctggagcca attaccctcg gtgcggtgct gattgccatt ctggtgttta
    23281 tcatcaccac gcagctggtg cgcaacttgc ccgcgctgct ggaactggcg attttgcagc
    23341 acctggattt aacgccgggt acgggttacg ccatcaccac catcaccaaa tatctgctga
    23401 tgctgattgg cgggctggtc ggcttctcaa tgattggtat tgagtggtcg aaattgcagt
    23461 ggctggttgc cgcgctcggt gttggtctcg gttttggttt gcaggaaatt ttcgccaact
    23521 ttatctctgg tctgattatc ctgttcgaaa aaccgattcg cattggcgat acggtgacaa
    23581 ttcgcgatct caccggtagc gtgacgaaaa ttaacacccg cgccaccacc atcagcgact
    23641 gggaccgtaa agagataatc gtgccgaaca aggcgtttat taccgagcag tttatcaact
    23701 ggtcgctctc tgactcggtc acgcgcgtgg tgttgacgat accggcccct gccgatgcca
    23761 atagcgaaga agtgacggaa atcctgctca ccgcagcgcg tcgctgctcg ctggtgatcg
    23821 acaacccggc accggaagtc ttcctggtgg atctgcaaca ggggattcag attttcgagc
    23881 tgcgtattta cgccgctgag atgggtcacc gtatgccgct acgccatgag atccaccagc
    23941 tgattctggc tggcttccat gcccacggta tcgatatgcc attcccgccc ttccagatgc
    24001 gtctggaaag cctcaacggt aaacaaacgg ggagaacgct gacgtctgcg ggcaaaggtc
    24061 gtcaggcggg aagtttgtaa gtttgtgtgc cggatgaacc aatccggctc acaaacaaaa
    24121 ctgatagcgc agaaaaaact gccacagcca gtagccgaaa atagccagcg cggtggcgta
    24181 cataaaccag ccgcgttttt ttcgccacaa agcgaaccag agaaaaggga aaaagagcgg
    24241 taacgtcatt atcacaccaa catcacgggt atcgtcttct tcgagataca tcaacacatc
    24301 gcagacgttg ttaatcccgc gtccatcaat catccactct ttgtccagcg cggaagtgat
    24361 ggttatccag aggaacgcca caataaacca gatgcagcat aagacaaaca tgcgcgcact
    24421 cataaggtaa ccggtcgctt aatttcgcta tagcgcatac gaaacaaatc gccgtatccc
    24481 actaattccc cgcgaaaaac cagacatcgg ataagataag cgcaataaat atccggcagg
    24541 tttccccacc gtttaccatc gcgatctttg agggtgttaa tggtgctacc caccaccatc
    24601 gccactttac gggtgtggca ggcatcgaca aaagagagaa tcgtctgctc tatttccgct
    24661 acatccccgg cagaaaattg cagcgccctg atttcatctt cggttattgc cgggtcccgg
    24721 ctggtgtcat ccataacatt tcctgtcttt acgccctatc gtcaggtaga cgatagggca
    24781 tcgggttatt ccaccgccca ctgcggattt tttgccactc tgctgcaata gttctggtaa
    24841 atcgccatcg ctaacagcga gaagaagaca gggccgccaa tcatccacaa tgtgctgtcc
    24901 cagtctccgg cttcaaccac aggttgaatg atggtgaaga cgttcgcaaa tgtcaccacc
    24961 agtacgacaa ccactgtcgc aatcattgcc gacaaatgcg ttttgaaaat cacaaacggt
    25021 ctgtcgagat cctgacgtgc tttaaagaac gggaaagcca gcgcgaggaa caggtaagga
    25081 agcgtcatag acacgttcgc catcagcgtc agcttgttaa agaacgccga tgcggtaccg
    25141 ccaccaaacg aaaccagcag gatgaagaca gtaaccaacc cgcactgcat ccacatggcg
    25201 atagacggca tccccatcgc attcaggcgc gtcatcggtt ccggccacaa tgctttcggc
    25261 gtcccctgga tgatggcttt caacggtgaa tagcaaagcg taaagaacgc accggtatag
    25321 gcgaggaaca tcgaaagtcc agtaatacgc gcaaaccata cgcccagcga caatgacgct
    25381 tcaggtgaca aatgcagtgc attacccagc gtcatcccaa ggctcttcat cagcacatag
    25441 gtaatattgc cgaggttaac ggaaccatta cttaatacct gctgccagtt tgtgctgacg
    25501 ccccataaaa atattgccag cgaataaccg attgaaataa caatagcggc aaaaacaata
    25561 cctttggcaa agttcttttc tggattttcc gttttatcga ccagaccacc aaccgcttca
    25621 attccgccat aggcaaaaat ggcaaatacc acaaacgata gcattgccag accggactga
    25681 taacccgggt tcggtgatgc aaggaaatta atatcctgcg cgaaatgccc accatttaat
    25741 aacaaaatag taatgcttac taacagcaat actaaattca gacacattac tgcaataccg
    25801 cccaccgcag taatgcgggc aattttatta atccccttag aagcaacgac ggtgaccaga
    25861 atcatccatg ccactgccag cagaccaacc acctgcgtag gctccagtcc ggcaatacgc
    25921 cagtgctggg tcatgtcgct accatagagg aatgttgaga acggtaccca aactttcgcg
    25981 gaggtactca ccatccagat gatataagag gaaaaccaca taaacgtacc aatgaaggca
    26041 aaacgtggtc cgacactatt attcatccag gaatagatac cgccttcttc tttgcgataa
    26101 gcagctccca tttcagccat cattaaggcg aatggaataa agaataacaa tgcagaaaat
    26161 atataaaagg gaatcgcact ataacccatt aagtaataag ccgatgggct attggcaaat
    26221 ccaaatacgg aagtaaagat catcaatatg agtcctatca gactcatctt ttttatcgtg
    26281 tgaggcatga aaccatcctt catttcagcg cgtgtataac acgtgcccac tgaactgcac
    26341 tgcgcaacat tgagcaggca gcgtaaaaac caatacagaa ttgatacctg gatgatatca
    26401 aatatccagt cttaaattgt ggcctttaac ggaaaaatga aaacatatgc tacagaatgg
    26461 cgcttatcac gccattcttc gctgttaatt cagtaatttt tcagaattat gcccggtcaa
    26521 cgctaaaggc gatgacttca gccagtgtct ccgcgcccag cgccaacatc accagacgat
    26581 caacacctaa tgccacgccg gaacagtcag gcataccgac tttcaaggct tcaatcagat
    26641 tctggtcaat ggggtgctgc ggcaaaccgc gcgccgcgcg cttacggtta tcttgttcaa
    26701 agcgttgttg ctgctcacgg gcatccgtca attcatggaa accattcgcc agctcaatac
    26761 ctttataata aacctcaaag cgttcagcga cccgatgatc ttcggtactg atttgcgcca
    26821 gtgatgcctg gctggctgga aagtggtaca caaaggtcgg tttttctttg ccaatatttg
    26881 gctctacgcc aaaggtaaac agcaattgta gcagcgtgtc gcggtcttct tcggtatcag
    26941 caacattgct caaatccagt ttcgctgcga cttcccgcag ttgcgttttg tcggcagaga
    27001 gcgggtcaat ttccagataa cgcaagaaag cttgttgata agaaaggctt tctgctgccg
    27061 ggcagtccag cacctgttgt aagagatcgt ccacctcgtt catcaaccgg tacatatcat
    27121 agtgcggtcg ataccactcc agcatagtga actcagggtt gtgataacgc cccatctctt
    27181 cattacggaa gctgcggcac agctggaata ccggcccaca accggcaacc agcaggcgtt
    27241 tcatatggta ttccgggctg gtcattaacc agagattcat cccctgcgaa tgcccggggc
    27301 caacgaaacg tgtctcaaac gggaccaaat gaatatcggt taccgtcgcc tggctcatac
    27361 aaggcgtctc cacctccagc actccacgat cggcaaagaa acgacggatc tccgccataa
    27421 tcgccgcgcg ttttaataag ttaggaatgg atgcgctcgg ctgccaggat gccgtttcgc
    27481 tcatagttaa atctccagtt tttgacaagg gcacgaagtc tactcgcaac gcgacggcga
    27541 gacaaatttt acgcaggaat caaacagcgg tgggcagtga ctaaaaaaag cacgatctga
    27601 tggtttagta attaaattaa tcatcttcag tgataattta gccctcttgc gcactaaaaa
    27661 aatcgatctc gtcaaatttc agacttatcc atcagactat actgttgtac ctataaagga
    27721 gcagtggaat agcgttcgca gaccgtaact ttcaggcact taccctgaag tacggggctg
    27781 tgggataaaa acaatctgga ggaatgtcgt gcaaaccttt caagccgatc ttgccattgt
    27841 aggcgccggt ggcgcgggat tacgtgctgc aattgctgcc gcgcaggcaa atccaaatgc
    27901 aaaaatcgca ctaatctcaa aagtataccc gatgcgtagc cataccgttg ctgcagaagg
    27961 gggctccgcc gctgtcgcgc aggatcatga cagcttcgaa tatcactttc acgatacagt
    28021 agcgggtggc gactggttgt gtgagcagga tgtcgtggat tatttcgtcc accactgccc
    28081 aaccgaaatg acccaactgg aactgtgggg gtgcccatgg agccgtcgcc cggatggtag
    28141 cgtcaacgta cgtcgcttcg gcggcatgaa aatcgagcgt acctggttcg ccgccgataa
    28201 gaccggcttc catatgctgc acacgctgtt ccagacctct ctgcaattcc cgcagatcca
    28261 gcgttttgac gaacatttcg tgctggatat tctggttgat gatggtcatg ttcgcggcct
    28321 ggtagcaatg aacatgatgg aaggcacgct ggtgcagatc cgtgctaacg cggtcgttat
    28381 ggctaccggc ggtgcgggtc gcgtttatcg ttacaacacc aacggcggca tcgttaccgg
    28441 tgacggtatg ggtatggcgc taagccacgg cgttccgctg cgtgacatgg aattcgttca
    28501 gtatcaccca accggtctgc caggttccgg tatcctgatg accgaaggct gccgtggtga
    28561 aggcggtatt ctggtcaaca aaaatggcta ccgttatctg caagattacg gcatgggccc
    28621 ggaaactccg ctgggcgagc cgaaaaacaa atatatggaa ctgggtccac gcgacaaagt
    28681 ttctcaggcc ttctggcacg aatggcgtaa aggcaacacc atctccacgc cgcgtggtga
    28741 tgtggtttat ctcgacctgc gtcacctcgg cgagaaaaaa ctgcatgaac gtctgccgtt
    28801 catctgcgaa ctggcgaaag cgtacgttgg cgtcgatccg gttaaagaac cgattccggt
    28861 acgtccgacc gcacactaca ccatgggcgg tatcgaaacc gatcagaact gtgaaacccg
    28921 cattaaaggt ctgttcgccg tgggtgaatg ttcctctgtt ggtctgcacg gtgcaaaccg
    28981 tctgggctcc aactccctgg cggaactggt ggtcttcggc cgtctggccg gtgaacaagc
    29041 gacagagcgt gcagcaactg ccggtaatgg caacgaagcg gcaattgaag cgcaggcagc
    29101 tggcgttgaa caacgtctga aagatctggt taaccaggat ggcggcgaaa actgggcgaa
    29161 gatccgcgac gaaatgggcc tggcaatgga agaaggttgc ggtatctacc gtacgccgga
    29221 actgatgcag aaaaccatcg acaagctggc agagctgcag gaacgcttca agcgcgtgcg
    29281 catcaccgac acttccagcg tgttcaacac cgacctgctc tacaccattg aactgggcca
    29341 cggtctgaac gttgctgaat gtatggcgca ctccgcaatg gcacgtaaag agtcccgcgg
    29401 cgcacaccag cgtctggacg aaggttgcac cgagcgtgac gacgtcaact tcctcaaaca
    29461 caccctcgcc ttccgcgatg ctgatggcac gactcgcctg gagtacagcg acgtgaagat
    29521 tactacgctg ccgccagcta aacgcgttta cggtggcgaa gcggatgcag ccgataaggc
    29581 ggaagcagcc aataagaagg agaaggcgaa tggctgagat gaaaaacctg aaaattgagg
    29641 tggtgcgcta taacccggaa gtcgataccg caccgcatag cgcattctat gaagtgcctt
    29701 atgacgcaac tacctcatta ctggatgcgc tgggctacat caaagacaac ctggcaccgg
    29761 acctgagcta ccgctggtcc tgccgtatgg cgatttgtgg ttcctgcggc atgatggtta
    29821 acaacgtgcc aaaactggca tgtaaaacct tcctgcgtga ttacaccgac ggtatgaagg
    29881 ttgaagcgtt agctaacttc ccgattgaac gcgatctggt ggtcgatatg acccacttca
    29941 tcgaaagtct ggaagcgatc aaaccgtaca tcatcggcaa ctcccgcacc gcggatcagg
    30001 gtactaacat ccagaccccg gcgcagatgg cgaagtatca ccagttctcc ggttgcatca
    30061 actgtggtct gtgctacgcc gcgtgcccgc agtttggcct gaacccagag ttcatcggtc
    30121 cggctgccat tacgctggcg catcgttata acgaagatag ccgcgaccac ggtaagaagg
    30181 agcgtatggc gcagttgaac agccagaacg gcgtatggag ctgtactttc gtgggctatt
    30241 gctccgaagt ctgcccgaaa cacgtcgatc cggctgcggc cattcagcag ggcaaagtag
    30301 aaagttcgaa agactttctt atcgcgaccc tgaaaccacg ctaaggagtg caacatgacg
    30361 actaaacgta aaccgtatgt acggccaatg acgtccacct ggtggaaaaa attgccgttt
    30421 tatcgctttt acatgctgcg cgaaggcacg gcggttccgg ctgtgtggtt cagcattgaa
    30481 ctgattttcg ggctgtttgc cctgaaaaat ggcccggaag cctgggcggg attcgtcgac
    30541 tttttacaaa acccggttat tgtgatcatt aacctgatca ctctggcggc agctctgctg
    30601 cacaccaaaa cctggtttga actggcaccg aaagcggcca atatcattgt aaaagacgaa
    30661 aaaatgggtc cagagccaat tatcaaaagt ctctgggcgg taactgtggt tgccaccatc
    30721 gtaatcctgt ttgttgccct gtactggtaa ggagcctgag atgatcaatc caaatccaaa
    30781 gcgttctgac gaaccggtat tctggggcct cttcggggcc ggtggtatgt ggagcgcaat
    30841 tattgcgccg gtgatgatcc tgctggtggg tattctgctg ccgctgggac tgtttccggg
    30901 tgatgcactg agctacgagc gcgttctggc gttcgcgcag agcttcattg gtcgcgtatt
    30961 cctgttcctg atgatcgttc tgccgctgtg gtgtggttta caccgtatgc accacgcgat
    31021 gcacgatctg aaaatccacg tacctgcggg caaatgggtt ttctacggtc tggctgctat
    31081 cctgacagtt gtcacgctga ttggtgtcgt tacaatctaa cgcatcgcca atgtaaatcc
    31141 ggcccgccta tggcgggccg ttttgtatgg aaaccagacc ctatgttcaa aacgacgctc
    31201 tgcgccttat taattaccgc ctcttgcttc acatttgctg ccccccaaaa aatcaacgat
    31261 attgtgcatc gcacaattac cccgcttata gagcaacaaa agatccccgg tatggcggtg
    31321 gcggtaattt atcagggtaa accttattac tttacctggg gctatgcgga catcgccaaa
    31381 aagcagcccg tcacacagca aacgttgttt gagttaggtt cggtcagcaa aacatttacg
    31441 ggcgtgcttg gtggcgacgc tattgctcga ggggaaatca agttaagcga tcccgcaaca
    31501 aaatactggc ctgaacttac cgctaaacag tggaacggga tcacactatt acatctcgca
    31561 acctacaccg ctggcggcct gccattgcag gtgccggatg aggtgaaatc ctcaagcgac
    31621 ttgctgcgct tctatcaaaa ctggcagcct gcatgggctc caggaacaca acgtctgtat
    31681 gccaactcca gtatcggttt gttcggcgca ctggctgtga agccgtctgg tttgagtttt
    31741 gagcaggcga tgcaaacacg tgtcttccag ccactcaaac tcaaccatac gtggattaat
    31801 gtaccgcccg cagaagaaaa gaattacgcc tggggatatc gcgaaggcaa ggcagttcat
    31861 gtttcgccag gggcgttaga tgctgaaact tatggtgtga agtcgaccat tgaagatatg
    31921 gcctgctgga tacgaagcaa tatgaatccc cgtgatatca acgacaaaac acttcagcaa
    31981 gggatacaac tggcacaatc tcgctactgg caaaccggcg atatgtatca gggcctgggc
    32041 tgggaaatgc tggactggcc ggtaaatcct gacagcatca ttaacggcag tggcaataaa
    32101 attgcactgg cagcacgccc cgtaaaagcg attacgcccc caactcctgc agtacgcgca
    32161 tcatgggtac ataaaacagg ggcaaccggc ggatttggta gctatgtcgc gtttattcca
    32221 gaaaaagagc tgggtatagt gatgctggct aacaaaaact atcccaatcc agcgagagtc
    32281 accgccgcct ggcagattct caacgctcta cagtaaaatt tcatcgggtc cgaattttcg
    32341 gaccttttct gcgcttttcc ttgctgtcat ctacacttag aaaaaaacca gtaaggaaac
    32401 atttatgcgc ctgctccctc tcgttgccgc agcgacagct gcatttctgg tcgttgcctg
    32461 cagttctcct acgccgccgc gtggcgtgac agtagtgaat aatttcgacg ctaaacgcta
    32521 tctcggtacc tggtatgaga ttgcccgttt tgatcaccgt tttgaacgtg gactggaaaa
    32581 agtcaccgca acatacagtc tgcgtgatga cggcggcctg aatgtcatca ataaaggcta
    32641 taaccctgac agagaaatgt ggcagcagag tgaagggaaa gcgtacttta ccggcgcacc
    32701 aacacgcgct gcgctgaaag tgtcattctt tggtcctttc tatggcggtt ataacgtcat
    32761 tgcacttgat cgggaatacc gccatgcgct ggtttgcggc ccggaccgcg actacctgtg
    32821 gatactctcc cgcacgccaa ccatttctga cgaagtgaaa caggagatgc tggcagtcgc
    32881 gacccgggaa gggtttgatg tcagtaagtt tatttgggta cagcagcccg gtagttagtg
    32941 agtgctgagt ttcagaccaa taatccccaa tacgattaac gccagactcg ccaggcgcat
    33001 cgggttagcg gactcaccga gcagtacaat gccggtgata gccgcgccga ctgcgccaat
    33061 acccgtccac acggcataag ccgtccctac tggtaacgat ttcatcgccc aggcaagtag
    33121 cgccatactg acaatcatcg ccgtcacggt aataacactc ggcgtcaaac gactaaagcc
    33181 gtgggtatat ttcaggccaa cggcccatac cacttccagc agaccagcaa taactaagat
    33241 aatccaggac atatcaggct ccggagaatt ggggccgtcc ccggtgaaaa gacgcgtttg
    33301 caggtcgtcc tgcaaagctg atggttgaac ggatattttg ccaggtggca ggggaatttc
    33361 aactattacc ttagtgacta tcgcagaaaa aacaaacggc acgacacagc tgtcgtaccg
    33421 ttattgctta ttgctattgc tgcgctttcg ttgctgcgcc agaaatcgcg ttaccgccat
    33481 cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc agttaatact gttgaaagca
    33541 ccagaacaga aaagatcgct gcaattgtct tcttcaccat aacgtcttcc ttttaggtaa
    33601 tgttatttat gtttgcctat agcaaattaa taatagacaa catcgccaaa aatgacggaa
    33661 atctgatgat ttttcggaag agaagaaaaa ttagctggca gcgcgagaga tggagttgcc
    33721 gagatgtttg atgtcttcgc cgaaaccgcg agcggtgtta cagcccgtga gcagcgtgct
    33781 ggcaagcaaa acaagaacga taaggcgttt catcatctct gacctgaata agtgatggtg
    33841 cagcctgcag gccgcaccac aaccgcatta cttcacgcga gagacgtatt caccagagcg
    33901 ggtatccact ttgatgactt cgccgatttg tacgaacagc ggaaccttaa ccacagcgcc
    33961 agtagacagg gtagccggtt tgccgccagt acctgcggta tcacctttca ggcccggatc
    34021 ggtatcaacg atttccagtt caacgaagtt cggcggagta acggagatcg gctgaccatt
    34081 ccacagagtt acgatacact ctgcctgatc cagcagccat ttagcgttat cgccaattgc
    34141 ttttgcatca gcagacagct gctcgaaagt ttcgttgttc atgaagtgcc agaactcacc
    34201 gtcgttgtac aggtaagtca ggttcatatc gacaacatca gcgccttcag cggaatcagt
    34261 agatttgaag gttttttcta cgcgagtacc ggtcagcaga cgacgcagtt taacgcgagc
    34321 aaatgcctgg cctttacccg gttttacgaa ttcactcgct tcaaccgcgt aaggttcgcc
    34381 gtctaacatg attttaagac cagcacgaaa atcgttgcta tagtacgttg ccataaggcc
    34441 ctctgaaatt tgttaattgg tagctaagcc acaaaatggc gcatattgta accctaaata
    34501 ccccatccag agaagattgg ttaacgcaac ttgccgatgt tgtgaccgat cctgatgaac
    34561 ttctgcgtct tttgaatata gacgcggacg aaaaactgtt ggccggacgc agcgccaaaa
    34621 agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg catggagaaa ggcaatccgg
    34681 acgatcctct tttgcgtcag gtacttacct cgcaagatga gtttgtcgtc gcgtccggat
    34741 tctccaccga cccactggaa gaacagcaca gcgtagtgcc tggtttgttg cataaatacc
    34801 acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt aaattgccgc tattgcttcc
    34861 gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg taactggcag actgcacttg
    34921 agtatgttgc tgcgcatccg gaactggacg agatgatttt ctctggcggc gatccgctga
    34981 tggcgaaaga tcacgagctg gactggttgc tcacacaact ggaagccatc ccgcatatca
    35041 aacgtctgcg gattcacagc cgtctgccga ttgtgatccc ggcacgcatc acagatgcgc
    35101 tggttgaacg cttttcacat tctacgctgc aaatcttgct ggtgaatcac atcaaccatg
    35161 ccaatgagat agatgaaaca ttccgtcagg cgatggctaa gttgcgccgg gtaggcgtta
    35221 ctttgctgaa ccagagcgtt ctgttacgtg atgtgaacga taacgcacaa acgctggcaa
    35281 acctgagtaa tgcgttgttc gatgccggcg tgatgccgta ttatctgcat gtgctcgata
    35341 aagtacaggg cgcggcgcat tttatggtga gtgatgacga agcacggcag attatgcgtg
    35401 agttgctgac attggtgtcg ggatatctgg tgccgaaact ggcgcgagaa attggcggcg
    35461 aacccagcaa aacgccgctg gatctccagc tacgccagca gtaactgatt aaacagggaa
    35521 gttaaacatt aaaattgcct gatgcgctac gcttatcagg cctacatcgt gcattgaaat
    35581 tgaatggatt tgcaatgaac tgtaggccgg ataaggcgtt tacgccgcat ccggcaagag
    35641 caacaaacgc aagtacagca acctgtcatt aaactgagaa atttaatctt tgcacgttgc
    35701 ataaccttta attattcata tgcattgctg ttgtta
//
LOCUS       DAPHBJ010000024        35447 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.024, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000024 DAPHBJ010000000
VERSION     DAPHBJ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.024"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(31..1890)
                     /gene="gss"
                     /locus_tag="RHZ76_001104"
     CDS             complement(31..1890)
                     /gene="gss"
                     /locus_tag="RHZ76_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HDV2811778.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2095..2961
                     /gene="yghU"
                     /locus_tag="RHZ76_001105"
     CDS             2095..2961
                     /gene="yghU"
                     /locus_tag="RHZ76_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HDV2811779.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3084..3332)
                     /gene="hybG"
                     /locus_tag="RHZ76_001106"
     CDS             complement(3084..3332)
                     /gene="hybG"
                     /locus_tag="RHZ76_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HDV2811780.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3345..3686)
                     /gene="hypA"
                     /locus_tag="RHZ76_001107"
     CDS             complement(3345..3686)
                     /gene="hypA"
                     /locus_tag="RHZ76_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDV2811781.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3679..4167)
                     /gene="hybE"
                     /locus_tag="RHZ76_001108"
     CDS             complement(3679..4167)
                     /gene="hybE"
                     /locus_tag="RHZ76_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HDV2811782.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4160..4654)
                     /locus_tag="RHZ76_001109"
     CDS             complement(4160..4654)
                     /locus_tag="RHZ76_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HDV2811783.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4654..6357)
                     /gene="hybC"
                     /locus_tag="RHZ76_001110"
     CDS             complement(4654..6357)
                     /gene="hybC"
                     /locus_tag="RHZ76_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HDV2811784.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLERG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6354..7532)
                     /gene="hybB"
                     /locus_tag="RHZ76_001111"
     CDS             complement(6354..7532)
                     /gene="hybB"
                     /locus_tag="RHZ76_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HDV2811785.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7522..8508)
                     /gene="hybA"
                     /locus_tag="RHZ76_001112"
     CDS             complement(7522..8508)
                     /gene="hybA"
                     /locus_tag="RHZ76_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HDV2811786.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPQYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8511..9629)
                     /gene="hybO"
                     /locus_tag="RHZ76_001113"
     CDS             complement(8511..9629)
                     /gene="hybO"
                     /locus_tag="RHZ76_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HDV2811787.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9818..10105)
                     /locus_tag="RHZ76_001114"
     CDS             complement(9818..10105)
                     /locus_tag="RHZ76_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HDV2811788.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10224..11109)
                     /locus_tag="RHZ76_001115"
                     /pseudo
     CDS             complement(10224..11109)
                     /locus_tag="RHZ76_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
     gene            11266..12306
                     /gene="gpr"
                     /locus_tag="RHZ76_001116"
     CDS             11266..12306
                     /gene="gpr"
                     /locus_tag="RHZ76_001116"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HDV2811789.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLREWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTKELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12346..12840)
                     /locus_tag="RHZ76_001117"
     CDS             complement(12346..12840)
                     /locus_tag="RHZ76_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HDV2811790.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13031..13915
                     /gene="yghA"
                     /locus_tag="RHZ76_001118"
     CDS             13031..13915
                     /gene="yghA"
                     /locus_tag="RHZ76_001118"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDV2811791.1"
                     /translation="MSHLKDPTTQYYTCEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14187..14612)
                     /gene="exbD"
                     /locus_tag="RHZ76_001119"
     CDS             complement(14187..14612)
                     /gene="exbD"
                     /locus_tag="RHZ76_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HDV2811792.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14619..15353)
                     /gene="exbB"
                     /locus_tag="RHZ76_001120"
     CDS             complement(14619..15353)
                     /gene="exbB"
                     /locus_tag="RHZ76_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDV2811793.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15605..16792
                     /gene="metC"
                     /locus_tag="RHZ76_001121"
     CDS             15605..16792
                     /gene="metC"
                     /locus_tag="RHZ76_001121"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HDV2811794.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16932..17591
                     /locus_tag="RHZ76_001122"
     CDS             16932..17591
                     /locus_tag="RHZ76_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV2811795.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17631..18530)
                     /gene="yqhC"
                     /locus_tag="RHZ76_001123"
     CDS             complement(17631..18530)
                     /gene="yqhC"
                     /locus_tag="RHZ76_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HDV2811796.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18724..19887
                     /gene="yqhD"
                     /locus_tag="RHZ76_001124"
     CDS             18724..19887
                     /gene="yqhD"
                     /locus_tag="RHZ76_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDV2811797.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19992..20819
                     /gene="dkgA"
                     /locus_tag="RHZ76_001125"
     CDS             19992..20819
                     /gene="dkgA"
                     /locus_tag="RHZ76_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HDV2811798.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21019..21945
                     /locus_tag="RHZ76_001126"
     CDS             21019..21945
                     /locus_tag="RHZ76_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HDV2811799.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21996..22253
                     /gene="yqhH"
                     /locus_tag="RHZ76_001127"
     CDS             21996..22253
                     /gene="yqhH"
                     /locus_tag="RHZ76_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HDV2811800.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22296..24515)
                     /locus_tag="RHZ76_001128"
     CDS             complement(22296..24515)
                     /locus_tag="RHZ76_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDV2811801.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24626..26038)
                     /gene="ftsP"
                     /locus_tag="RHZ76_001129"
     CDS             complement(24626..26038)
                     /gene="ftsP"
                     /locus_tag="RHZ76_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HDV2811802.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMNDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26113..26850)
                     /gene="plsC"
                     /locus_tag="RHZ76_001130"
                     /gene_synonym="parF"
     CDS             complement(26113..26850)
                     /gene="plsC"
                     /locus_tag="RHZ76_001130"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HDV2811803.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPTDAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27084..29342)
                     /gene="parC"
                     /locus_tag="RHZ76_001131"
     CDS             complement(27084..29342)
                     /gene="parC"
                     /locus_tag="RHZ76_001131"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDV2811804.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29480..31087)
                     /locus_tag="RHZ76_001132"
     CDS             complement(29480..31087)
                     /locus_tag="RHZ76_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV2811805.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQSAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(31220..31615)
                     /gene="mqsA"
                     /locus_tag="RHZ76_001133"
     CDS             complement(31220..31615)
                     /gene="mqsA"
                     /locus_tag="RHZ76_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MqsA"
                     /protein_id="HDV2811806.1"
                     /translation="MKCPVCHQGEMVSGIKDIPYTFRGRKKVLKGIHGLYCVHCEESI
                     MNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKKLSLTQKEASEIFGGGVNAFSRY
                     EKGNAQPHPSTIKLLRVLDKHPELLNEIR"
     gene            complement(31617..31913)
                     /gene="mqsR"
                     /locus_tag="RHZ76_001134"
     CDS             complement(31617..31913)
                     /gene="mqsR"
                     /locus_tag="RHZ76_001134"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin MqsR"
                     /protein_id="HDV2811807.1"
                     /translation="MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCN
                     VIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQVYLKITVIHDVLIVSFKEK"
     gene            complement(32118..32600)
                     /locus_tag="RHZ76_001135"
     CDS             complement(32118..32600)
                     /locus_tag="RHZ76_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV2811808.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTVWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(32653..33045)
                     /gene="ygiW"
                     /locus_tag="RHZ76_001136"
     CDS             complement(32653..33045)
                     /gene="ygiW"
                     /locus_tag="RHZ76_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HDV2811809.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            33197..33856
                     /gene="qseB"
                     /locus_tag="RHZ76_001137"
     CDS             33197..33856
                     /gene="qseB"
                     /locus_tag="RHZ76_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HDV2811810.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            33853..35202
                     /gene="qseC"
                     /locus_tag="RHZ76_001138"
     CDS             33853..35202
                     /gene="qseC"
                     /locus_tag="RHZ76_001138"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HDV2811811.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNRLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQISDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(35248..>35447)
                     /locus_tag="RHZ76_001139"
     CDS             complement(35248..>35447)
                     /locus_tag="RHZ76_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2645 domain-containing protein"
                     /protein_id="HDV2811812.1"
                     /translation="PSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWVYVF
                     SLGICLVCGWWLFWGRFIFCY"
BASE COUNT         8709 a         9436 c         8926 g         8376 t
ORIGIN      
        1 ataatcagta cggggttgct acaacatgta ttactttttc accacaatta acggttcaat
       61 atcactctct tttttgatga ccagtgattc atcaccgcgc aaacacgtcc caccgtagtt
      121 gccgccaacg gtgaaggtac atacctgaat gtatttaccg tccactttcg gcaaacacca
      181 cagttgctga tagatgtttt tctgctcggc aaatttaccg ctggttttgt ccagcacctc
      241 ttcatgatgg ctgacgaggt cgatattgct gccacagcga ccggcgatcg gtttcactgc
      301 gtaacctgtt ttcaccagtt catcattaac agtgaaatcg gtatccagca ggtaacgatg
      361 gtgcgggaac agcgaccaga ggatcggcag aatcgctttg ttgccgggga tcaccgtcca
      421 cagcggctca aagaccagca cttccgggcg cagcaatacg tcgataagac gcacttcgtt
      481 ttgcggatga ccggtacgga ttggcaccgc agcaaactca cggtcgctaa cttcacgaat
      541 ctgatcaaac gcggtttccc acgcccaggt tttccacacg cagttaacca gtcgcccttc
      601 cccatcaatc agttgcccgg cagcatccca gcccagttca tccaatccac gcaagatacg
      661 cgtttcaaag cccgcctggt gcagcgcctg ctccataaac tgcgcgtgat agttttcctc
      721 gatatctttg tcctgcatga tatggacaaa cggacgtgca cgactgtgtt tccaggcacc
      781 agccaattcg ttaatcagcc cttccgccgg attgaagccg ttgcctttat agccctgctc
      841 cgcccaacgt tcgaggatca agcccgcttc ggtatgacag gaggcggagt cggcgttgta
      901 ctcgtaaacc ttcaggccac gctcatccat gcagaaatcc atacgaccag tgatcatatg
      961 gtgacggcga cgctgccagg agagacgcaa acgtggccag aggattttcg ggatgtcgaa
     1021 cagcgccagc aggttgtcat ctttcagcac cttgtcggtt gcgtgaagat acatcaggtg
     1081 cagctcgttg gtggctttaa ttagctcctg ctcggcactc tcggtaatgg tgtagtaatg
     1141 ataaggatcc tgattgatca cctgaccgtt ggcctgcaca taggcgtttt gcagcggatc
     1201 tttttcatcc agccatttac cgtcaaactg gcctttgttt tccaggcgcg ctccgctgat
     1261 tttcagcagc tcgcctgcaa tttccggctg cggtaagctg tattcagtat cttccgtctg
     1321 gatcatccag cccagaatgg tggtgtcatc aaaagtgtct ttcagggtat agcagccgtt
     1381 ttcgaccacc atctccagct cgcgcgtcca ctgttgccct tgcggcaacg gggaatgaat
     1441 cacgttctgt tccgcaatac ggactttgtt gccatgcaat tgggtaatga tggcgacatg
     1501 gccagtgtct ttaaattcac cgcctttatc ccagataaga agcgcacccg cgaccggcgc
     1561 acgcggcgag ccgttaggaa atgcctgcaa tggcaggatg ttgtcattaa ccacttctcg
     1621 caggaagcgc agcgagaaaa tctcccacgc catacccacg tcagtaaaga ccacaccgta
     1681 attcagaaag agaaaacggc gagcaaattc aacgcattgc cacttgtggc ccatatattc
     1741 gtcgtcgata tagctacgga atacggcgtc atcttcgtat tcctgcggat cgagagaact
     1801 gtaatctgaa gagtagattg ctaccccacc tggggcgtag cccaataatg tcccgaacgg
     1861 ggcatcctgg ctggtcgttc ctttgctcat cactttacct ttaacaatac aacctaagca
     1921 gttggcgtaa ttgttgtgct ctgattacct gctcagcgac attaaccgga cagaggtcag
     1981 actaatcata cacctcatcg cagcggctgg cgcaaagggt taaaaaattc acattctgta
     2041 cagcaagtga cctgctacac tgcttcaaca ctaccactca gaaggcaact cactatgaca
     2101 gacaatactt atcagcccgc gaaagtctgg acgtgggata aatccgctgg cggcgcgttc
     2161 gccaatatca atcgcccggt ttctggtccg acgcatgaaa aaacgctgcc cgttggcaaa
     2221 catccattgc aactttattc gctgggaacg ccgaacggtc agaaagtaac gattatgctt
     2281 gaggagctgc tggcgctggg cgttactggt gcagaatacg acgcctggct gattcgtatt
     2341 ggcgatggtg atcaattctc cagcggcttt gtcgaagtga acccaaactc gaagatcccg
     2401 gcgctgcgcg atcatacgca taatccgccg atccgcgtgt ttgaatctgg ttcgatcctg
     2461 ctttatctgg cggagaaatt tggctacttc ctgccgcagg atttggcaaa gcgtactgaa
     2521 acgatgaact ggctgttctg gttacagggc gcggcaccgt tcctcggcgg tggttttggt
     2581 cacttttacc attacgcacc ggtaaagatt gagtacgcca tcaaccgctt taccatggaa
     2641 gccaaacgtc tgctcgacgt gctggataag caactggcgc agcataagtt tgttgcgggc
     2701 gatgagtaca ccattgcgga tatggcgatt tggccgtggt ttggcaacgt ggtgttaggt
     2761 ggtgtgtatg atgccgctga gtttcttgat gcgggcagtt ataagcatgt acaacgctgg
     2821 gcgaaagaag taggcgaacg tccggcggtg aaacgtgggc gtattgttaa ccgcaccaac
     2881 ggaccgctga atgagcagtt gcatgagcgc catgacgcca gtgatttcga gacgaatacg
     2941 gaagataagc gtcaggggta agggttggtg ttcgtcgcag caagccatcc aggccggata
     3001 aggcgttcac gccgcatccg gcaatcgtgc acaatgcctg atgcgacgct gccgcgtctt
     3061 atcaggccta cgaaagctaa tcatcacgcg ctggtaatgt cgtaatccat ttggcgcagt
     3121 gcgtctaatg tggctttggc ttcatcttcg tcgatgatgc tcatggcaaa tccgacgtgt
     3181 accagcaccc actggcccag tagatcggca gggttacctt cacaaatcag ggcgatattc
     3241 acatcgcgct tgataccaca tacttcaacc tgcgcaagct ggtgaatatc ttcaccgaca
     3301 gccagcacct ggcctggaac gccaatacac attattaact ccggttattc aacttcaata
     3361 cttttgacga tcagcgaatc gccggtatcg acacgcaacc gctcgccgtg acagagcgga
     3421 cactgcgcat cgtgctgatg aatctccacc acctggctgc aatcccagca ccaggcctgg
     3481 gcgggtttat agacgatatg taaatcgcac ccttgcgcca ccgttccgtg gcagacaatt
     3541 tcaaaactaa aacggacggc gctctcctca acgcaggaga gtgcgccaat ttccagccac
     3601 acggcggtga cgcgcttaac atcgtgctgc tccgcctgcc gttggataat ttcaacggcg
     3661 ctctggcaaa gagacaactc atgcattttc gccactcctg cgaccaaaca gcagggcgcg
     3721 acgccctgcg tgtggtacat ccggattcgt gactggcagc gaaaggatca ttcgtgcgca
     3781 gtcatccgtc aggcgttggc cctcttcaat cgacatgctg tgcgaaagcg gcgacatcag
     3841 cgaacaggag agatattgcg aaacaccgtc cagttcgcca acggtaaagg tcatagtgcc
     3901 atacggcagt tgcagaccaa ttttttcact cactttgcgc agcggccaga gttgatccgg
     3961 gccggggaag ataactgcac tcagcatcca cggggtgatc acacaccccg tccactgacc
     4021 ttcgaacagc gtaaaatcag aaacatacac cggcattgaa ggatgcagaa aagagagatc
     4081 gtgcatcgaa cgccgggcaa tttcttcaaa cgctgcctgt acttgcgcct tcggggaggt
     4141 ctggaaacct gctatctctt cagtcatgaa tcgcctcccg tgggatggct tccacgccag
     4201 attcacgcag cgcagccaga acctgctcaa gcgcaggttc aatcattgct tcaaccgtcg
     4261 gcgttaagcc gatgtgtggc tccagcgatt ccgggatcac gccgaccagg gtcagctttt
     4321 tcggaaactc gccggtgaag cgcagggccg acaagacgtc ggccaggcca agctgatgcg
     4381 gagagatttt gttggtaaac aacgccggaa cttcttcatc ccgcaggatc atcatcgttc
     4441 ccggcgcgtt ctttttcgag acaatggcat ccgcaataat caaatgatcg cgatttgcca
     4501 tgtcgccaag cagctccatt cccgccgtgc cgccatcgag gatctcaaca taatccggca
     4561 gaatgtatcg ttgctctaac gcttcgacaa tccgcacacc gatggcttca tcggtcagca
     4621 aaatattgcc gacccctaag actaaaatac gcattacaga accttcactg aaaccacttc
     4681 gttgccgtca gcatccacta cgtgtaccgc acaggccatg cacgggtcaa aggagtgaat
     4741 ggtacgcacc acttccagcg gtttattcgg atcggcaacc ggtgtaccca ccagcgactg
     4801 ctcgtaagga ccgacgtcat cattgaagtt acgcggacca gagttccagg ttgatggaac
     4861 aaccgcctgg tagttgctga tgataccgtc tttaataacc atccagtgag agagcatacc
     4921 gcgcggcgct tcgaggaagc caacaccttt gaattcaccc gttgccggaa tgttcggttt
     4981 cacgaaggtg gtgtgatcgc ctttgccgat attggtgatc agtgcactgt attggttttg
     5041 caggatatcc tgcaattcgc agcagtgaac ggtacgacca ataatacggc ccagcgtaga
     5101 gtgcagttgc gccacttcca gcgtgttacc agtcagtttc tgataaatcg caacgatttc
     5161 attcagtttg ttctgggtag attcgcgacc tgccgccagt ttcaccagca tattagccag
     5221 cggacccact tctaccgttt tgccgtagaa agtcggtgat ttcacccagg aatatttccc
     5281 gtcgtcagac caaccatcat aagccggaat ggtggtacct tcccacggtg cctgcggcgc
     5341 ttcgtcttta taccaggagt gcttcgcgct ttcctgaatg cctttgatca ggtattcatc
     5401 ggaatgagaa gtgatcggac gatacgagga cagatccgca ttctcaatgt agccgccagg
     5461 gaacaggaag ctgccgtttt tactgtcggt cgggaattcc ggcacgctca ggtagttcac
     5521 cgcaccttta ccacgctcca gccattccgg gtagaacgca gcgataaccg cggtatcgac
     5581 cttgtaaacc tgttcaacaa agtcgctcag cttgtcgatg aaagacttga tgtacatcag
     5641 gcgctcaagg ttcagcacgc ccagaccgtc gaggttgatt gggttagcga ccccgcctac
     5701 cgccaggttc tgaatgtgcg gcgttttacc gcccagcagc gccacgacgc ggttagcgtc
     5761 acgctggcac tccagcgcct gcaggtagtg cgctaccgca atcaggttca cttccggcgg
     5821 cagtttcatt gccgggtgac cccagtagcc gttggcgaaa atccccaact gaccgctggc
     5881 aaccagatct ttgatcttgt tctgaacttt ggtgaactct tccgggctgt tcaggtgcca
     5941 ggtcgaaacg cctttcagca tttcagaggc tttggttggg tcagcttgca gtgcagaagt
     6001 gatgtccacc cagtccagcg ccgaaagctg atagaaatga acgatatggt catgcgtggt
     6061 gtgcgcagcc agaatgatgt tacggatgta ttgcgcgtta accggaacgt cgatattcag
     6121 cgcactttct gccgcacgaa cggaagacag cgcgtgagtg gtagtacata cgccacagat
     6181 acgttgcaca atcatccatg catcgcgcgg atcgcggttt ttcacgatct cttccatgcc
     6241 gcgccacatg gtaccggaag cccatgcttt cgaaacgacg ccattttcga tttcgcaatc
     6301 gatgcgtaaa tgcccctcaa tacgggttac cggatcaata gtaattctct ggctcatgct
     6361 ttgctcgcct catgacgatt atgatcgttt tgttttaaag gaggaagtat cggcagtaga
     6421 cgaatgagta cgatgtaagc gcaaatctca atagccacaa aaccaataga aatcaacagt
     6481 tcttcccagg tcgggaagta ggcgtaaccg ccgcccgggt tgaatgccac cagcgaatag
     6541 gtcagacgcc aggttgcaca acctaacagt gcgctcagtg ctgacaggaa cagcatgcgg
     6601 gaatcattac gcagcttcgc cacacgcaga acgaccagcg ggaagagcat cagcaggact
     6661 tcaatccaga acatcacgga gtagaagtca ccggcaaacg ctaacgacag cttgtcgcga
     6721 tagataagct cgccaaagcg cagcacgatg aaaatcgcca gcaacacact gatggtgttg
     6781 gtcagcttga cgaacagact cttttcatcc ggaccgttgc cacgcagacc cgcctgcacc
     6841 agcgaacctt caaagatgac aatcgagaag cccatgatga acgccgtcag cagcgagaac
     6901 agcggcaaca tttcatagct ctgccacaac ggatgcacct tgtagcccgc cgagatcatc
     6961 agtgacccca ttgaagactg gtgcatggtc ggcaacagcg cgccaagtgc aatgatgaag
     7021 aacatcacct tgttcagacg ctgtagcgac accttccagc ccagacgttc aaacagtgcc
     7081 ggagcaaact ccagagccat cacgccgatg tagatggtca tacagaccgc cgtctcgaac
     7141 agtaccgagt tcacgttgaa gtgacccgga atgtagaagt acggcaggtt ccagtagcga
     7201 cccacgtcga tagtgatcga caaaccaccc agtgagtaac caaacagact cgccaacagc
     7261 gccggacgca ccagcggatg gtattgccca cggttaaaga cgtataccgc ccacgccagc
     7321 gcccagccgc cacaggcaaa gccggtgcca atcagcaggt caaacgcgat ccacacgccc
     7381 cacgggaagc cgccgttcag gtcagagaca gagcccagac cgaacaccag acgcttcaca
     7441 atcaggagca tacagatgac gattaacggt ccaaaaatca tgaccggttt actgatgatt
     7501 ttgccgccca gcggttgtgg atcatgactc atgatcgtct cctccgtcgt gatggtcgtt
     7561 tttggtgttg cgacgaacca gcacggttaa gcccgccagc acagccagtg gtagcatcat
     7621 gcctttatac agggtgtgtt gaatattttc ggaacgcgca ccggtagaaa gatcgtccag
     7681 tttcggcagg tcgagatttt cataaggcac acccgtcagt accagaacct gagtaccgcc
     7741 gccctctttc tcgccgtaca gatgcggata atattgcggc accgtatgca ggtaagtgtc
     7801 gccagatttc agcgtctgac gcggatagtg gtattcgctg ccaggcttca gcgccagacg
     7861 ttttttcgcc tccgccatca gctcttcacg cgtaccgaaa atcaccgcgc ccgccgggca
     7921 cacttctacg cagccaggca gaccgccttt atcgagacgt tccacacctt tctggttgca
     7981 cagctcgcac ttatgcagcg caccaaacgg gttgttgtag tcgtacttcg gcacgttgta
     8041 cggacaggcg accatgcagt aacggcaacc ggtgcacacg tctttgtcgt aatggacaat
     8101 gccggttttc ggatcttttt tcagtgcaga gaccgggcac acagagacac agttcggatc
     8161 gacgcagtgc atacactgtt tcttaatgta cgcgtagccg ttctcctcct ggtctttgtt
     8221 gacccctgtg ccgctggtcc acacctgaat gatgttattg gtatacggcg acagtttgtc
     8281 gttgttcgac caggtctgtt ccccttgcgg gttacgttca gggaaattga tatcctgaca
     8341 cttggtgacg caagcctggc agcctacgca caaggtcgag tcgtacaaca tccccagcga
     8401 tcccggaatt ggcgggcggt tttcagcagc cgcatgactg acagacggca gcgcgcccgt
     8461 cagcaatgcc ccgcaggagg ctgctttaat aaaattacgt ctgttcacgg ttattctccc
     8521 cgtgagtcag cgttatcttt cttttgctga cgacccagtt cacgcaccgc catcacgctg
     8581 acaccggcaa ccaacccaac cacaccgccg agcaaaccaa tagcgcctgc agagacgttg
     8641 ccgccctctt tagcgttaac atccggtttc tgtgaacgcg gagtttgatt ttcgacgttg
     8701 gcaagctgat ggatgccttt atggaagccg ataccttctt cgttacagcc atagcacggg
     8761 tgaccaatcg ccaccggcca cacaccgcca acatcgcaga attgcagcgt tgagcagttg
     8821 ccgtaagttt ctggcccttt acagccgagg tggtacaggc accagccttc gcggtggcct
     8881 tcatcaccga actctttggc aaaacgacca gcatcgaagt gcgggcgacg ttcgcagtgt
     8941 tcgtgaatca gacggccata ggcgaaggtc ggacggtttt tgtcatccag tttcggcggt
     9001 ttgccgtaag tgatgatgtg cgcaacggtc gcgaggaagt tgtgcgggtt cggcgggcag
     9061 cccggaatat tgataacggt tttgcctggc agaacttctt gcaggctgac tgcgccagtt
     9121 gggttaactc cagctgcggc aacaccgccc cacgcagagc aggaaccgat agcgataatg
     9181 gctgctgcgc cttccgccgc tttgcggatg tgatccacaa tcggctcacc ggcaaccatg
     9241 caataaatac cgttatcttt taatgggatg gaaccatcca ccactaacac atactgccct
     9301 ttgtacttct cgagagcgtt atgtttgttc tcttcgacct gatgaccgaa ggcggcggaa
     9361 agcacttcgt gatactccag agagatagtc tccagtacga ggttttctac cgttggatgc
     9421 gttgcacgaa gcagagattc cgtacaaccg gtgcactcct gcgcgccaat ccagataact
     9481 ggcggacgct gcgggttagt aaccgattcg gccatctctg cagcggcttt gctacttaac
     9541 cccatggtgg cggctaatgc tgcacaaagc ttcatgaaat cacgacggtt aatgccgtga
     9601 gaatggatga gggtgttatc tccagtcatt tatagttatt ccgttgcgaa gacctggcat
     9661 atattttgcc tcaatcgcaa aatcaataat gcgatcgatg cgccatttac cacacattta
     9721 ttatggttat cgatatcatg atactgcggc ggggcgctaa aacgaaggga agaagcatta
     9781 gtgtaattaa ttaaagcaag ataatacgta tgtttgatca attttcgagg cgataaccgg
     9841 ccataaaaaa gcgcaacgta gaacaggaat tattctcacg aaagaaatcc attaccatct
     9901 ctttatccag tccatagcgg cgcgtcagaa tacccgcttc ccaggcgcta agctggcgat
     9961 ctccggtctt ttcgtacatc cggtgtgagt agcctaatac aaaaccgcgt ttataatcgg
    10021 cacagaattt tgtcacatta accgcactgt cggcatgcgt tgcttttaat cccgccatta
    10081 agcctttacc aaaatggtta ttcatcgttc acctcatttg ctatatatta gcatatatag
    10141 cgatatttta ggcaaagaac gacttttgcg cacgctattt tttcatgctt gtttaagacg
    10201 taatcccgta agagataaac ctatcaggag agatatttat cgaaccattt cagtgtcctt
    10261 tgccaggcaa gatcggcggc agatttgtca taacggggcg tggaatcatt atggaatccg
    10321 tgattaaccc ccggatagat atacgcctca taaaccttat tattggcttt caacgccgcc
    10381 tcgtaagcag gccagccctc gttgattcgg gtgtccagtt ccgcgaagtg gagtagtaaa
    10441 ggcgcttcaa tcttcgccac atcggcagtg ggtgcctgac gaccataaaa cggcaccgcg
    10501 caggccagtt ccggatacgc gacagccgcc gcgttcgata cgccaccgcc atagcaaaat
    10561 ccggtaatac ccactttgcc tgtcgcttgc ggatagcgtt gcataaactc aattgcggca
    10621 aagaaatcat tcatcagttt ggttggatca acctgctgtt gcagctcacg acctttatca
    10681 tcatttcccg gataacctcc aacggaactt aagccgtcag gtgccagggc gatatacccc
    10741 gccttcgcca ctcgccgtgc cacatcttcg atatacggat tcagtccacg attctcatgc
    10801 accaccacta cggctggcgt tttgccgctc atctttgcgg gcttcaccag ataaccccgc
    10861 acctcgccgt gaccatttgg cgaaggatac gtgatgtact cagcaacaat ttccgggtcg
    10921 gtaaactcta cctgagtcgc cagcgcataa tttggcttga gcaaatcaaa caacgctaat
    10981 gccgccatcc cgcctaccgc acttcgccgc aaggttgagg aactcacgtt tcgagatttt
    11041 cccgtgagcg taatagtcat agtaatccag caactcttgt gggaaatctt tggcggttaa
    11101 acgcggcatc gttgcactcc tcagttggtg tttttttaag caaagcataa gcacgtgttt
    11161 ttgcccagtt tttcgtcact ctgtgagcca gactacggga tacgcgctgg cgaatcgcta
    11221 aactagaaac attgtttcga aattgaacgg tggaaaggag aggtcatggt ctggttagcg
    11281 aatcccgaac gttacgggca gatgcaatac cgctattgcg gaaaaagtgg tttacgcctg
    11341 cccgcgttat cgctcggttt atggcacaat ttcggtcacg ttaacgcgct ggaatcacag
    11401 cgtgcgatcc tgcgtaaagc gtttgatttg ggcattacgc actttgattt agccaacaat
    11461 tacgggccgc ctccaggaag cgcagaagag aactttggtc gcctgctgcg ggaggatttt
    11521 gccgcttatc gcgatgaact gattatctct accaaggctg gctacgatat gtggcccggc
    11581 ccttacggct ctggcggttc acgtaaatac ctgctcgcca gcctcgacca aagcctgaag
    11641 cgtatggggc ttgagtatgt cgatatcttt tactctcatc gcgtcgatga aaatacgccg
    11701 atggaagaaa ccgcctctgc gctggctcat gcggtacaaa gcggtaaggc gctgtatgtc
    11761 gggatctcct cttactcgcc agagcggacg caaaaaatgg tcgagttgct gcgcgagtgg
    11821 aaaattccgc tgttaattca tcaaccttcg tacaatttac tgaaccgctg ggtggataaa
    11881 agcggcctgc tggataccct gcaaaataac ggcgtgggct gtattgcctt tactcctctg
    11941 gctcagggat tgctgaccgg aaaatatctc aacggcattc cgcaagattc acggatgcat
    12001 cgtgaaggga ataaagttcg tggtctgaca ccgaaaatgc ttaccgaagc caacctcaac
    12061 agcctgcgct tattgaatga aatggcacag cagcgtggac aatcaatggc gcaaatggcg
    12121 ttaagctggt tgctgaaaga tgatcgcgtg acgtcggtat tgattggtgc cagccgcgcg
    12181 gagcaactag aggagaacgt gcaggcgctg aataatctga catttagcac caaggagctg
    12241 gcgcagattg atcagcatat cgccgatggc gagctgaatc tgtggcaggc gtcttccgat
    12301 aaatgacctg ttaataacgg gcgcgcgaac cgcgcccgaa tcagatcagt gattatgacg
    12361 agtcagtcgg tcaagatagc ccatcacaaa tgcagaaagc acaaacgtca gatggataat
    12421 gacgtaccac atcagtttgt tatccgggac atttttcgca tccataaaga cgcgcagtaa
    12481 gtgaatggaa gaaattgcca caatcgacgc tgctactttg tttttcagcg acgttgcgtc
    12541 cattttcccc agccagttca gcttctcttt gttctcggag atatccagct gtgagacgaa
    12601 attctcataa ccggaaaaca tcaccatcac cagcaaaccg ccaaccagcg tcatatccac
    12661 cagcgacagc aacacgagga tcaaatctga ttccgccatc gagaagatat tcggcagtac
    12721 gtgaataatc tcctggaaga acttcagcgc cagggcaact aacgccagcg aaaggccaaa
    12781 gtacacgggg gcaagcagcc agcgagaagc atacattgca ttttcaagaa aacgttccat
    12841 acattcctgt cgtgtagtta aaacgggcaa cagtatatcg caattgagta aacagcacgc
    12901 aacagcaaat caacatatac gttaaacctt tgtcgcataa ttctgaggtt tagggatctt
    12961 cctggttccc ttatcaacgc atcgagaacg tctaccctta aaggacgttg aacctgaagg
    13021 gagaaaaacg atgtctcatt taaaagaccc gaccacgcag tattacactt gtgaatatcc
    13081 caaacagaaa caaccgacgc caggcatcca ggcgaagatg acaccggtac cggattgcgg
    13141 cgagaaaacc tatgttggta gcggtcgcct gaaagatcgt aaagcactgg tgacaggggg
    13201 cgattccgga ataggtcgcg ctgccgccat cgcttacgcg cgtgaagggg ctgacgtggc
    13261 gatcagttat cttcccgtgg aagaagaaga cgctcaggat gtgaaaaaga tcattgaaga
    13321 atgcggacgc aaagccgttc tgctgccagg cgatttaagc gatgagaaat ttgcccgttc
    13381 gctggttcac gaagcgcaca aggcgttagg cgggctggat attatggcgc tggtcgccgg
    13441 gaaacaggtt gccattccgg atattgcaga cctcaccagc gaacagtttc aaaagacctt
    13501 tgccattaac gttttcgcgc tgttctggct aacccaggaa gcgatccccc tgctaccgaa
    13561 aggtgcaagt atcatcacca cttcgtcaat ccaggcatac cagccaagtc cgcatttact
    13621 ggactatgcg gctacgaagg cggcgattct gaactacagc cgtggcttgg caaaacaggt
    13681 cgcggagaaa ggtattcggg tgaatattgt cgcgccaggc ccgatctgga cagcactgca
    13741 aatttccggc ggacaaacgc aggataagat cccgcagttt ggtcagcaaa cgccgatgaa
    13801 acgtgcgggg caaccggcgg aactggcccc tgtatatgtt tatctggcaa gtcaggagtc
    13861 gagctacgtc accgcagaag tgcacggcgt gtgcggcggc gagcatttag gttaaaaaaa
    13921 tgcccggttg tgaaaagcaa ccgggcatca ttgtgaattc actcttacct gatgtggcga
    13981 aaacgcctta tccgcctaca aacgcatgaa gattcaacat gttgcaatag attttgtagg
    14041 cctgataagc gtagcgcatc aggcattgag cacctaatgc cggatgcgtc gtgaacgcct
    14101 tatccggcct acaaacgcac gaagattcaa catgttgcaa tagattttgt aggactgatg
    14161 agcgtagcgc atcaggcaat tctactttac ttcgctttgg cggtttcttc gccgaccaga
    14221 cctatcttca ggtaacccgc ctgatgcagc gtatccatta ccttcatcaa cgtctcgtaa
    14281 tcgacggttt tgtccgctcg gaagaagatg gtggtgtctt tcttgccttc ggttaacgca
    14341 ttcaacgccg taatcattgt ttcatcggtg accggatcgt taccgataaa catcgagttg
    14401 tctgccttca ccgacagata aaccggtttt tccgggcgcg gctgcggcgt gctggtagaa
    14461 gcaggcaagt tcaccttcac atctaccgtc gctaacggtg ccgccaccat aaagataatc
    14521 agcagaacca acatcacgtc gataaacggc gtcacgttga tatcatgcat ttcgccgtta
    14581 tcgtcgaggt tttcgttaag atgcattgcc atcggatatt atcctgcgcg taatttttgt
    14641 gcgacacgaa ccggatgcgc agcggcgctg gcttccagat ccaggtcacg gctttgcagc
    14701 aacaatacct gcgctgcaac atcacccagc atcgctttaa agccgccaat ctggcgtgca
    14761 aatacgttat agataacgac cgcaggaatc gctgccacga ggccgattgc cgttgctaac
    14821 agagcttctg cgatacccgg cgcaacgact gccaggttag tggtctgcgt ttgcgcgata
    14881 ccaataaagc tgttcatgat gccccagacc gtaccaaaca gaccaacgaa cggagaaatc
    14941 gcgccgatgg ttgccagata gccgttaccg cggcccattt gacgacccac tgcggcgacc
    15001 cgacgctcca gacggaagct ggtacgttct ttaatacctt cgttatcgtc gctgccttct
    15061 gacagttcca gctcattctg cgcttcattg agcaaatgca ggcttaagct tttgctacca
    15121 aaatcagcgg cgatatcgtt ggcctggttt aaggaacgcg cttcagccag cagttgctgc
    15181 tcgcgcttaa ggcgacgctt ctgattgaag aattctacgc tcttactgaa gaagattgcc
    15241 caagtgacta cggaggccaa aataagccca atcatcacgc acttaacgac aatatcggcg
    15301 tgctgataca taccccagac ggaaaggtcc gtctgcatca aattattacc cactgtgtat
    15361 ctccaggacg caagtcacaa aatctgcgca taataatatc aaaatgacgt cgaattgata
    15421 gtcgttctca ttactatttg catactgccg tacctttgct ttcttttcct tgcgtttacg
    15481 cagtaaaaaa gtcaccagca cgccatttgc gaaaattttc tgctttatgc caattcttca
    15541 ggatgcgccc gcgaatattc atgctagttt agacatccag acgtataaaa acaggaatcc
    15601 cgacatggcg gacaaaaagc ttgatactca actggtgaat gcaggacgca gcaaaaaata
    15661 cactctcggc gcggtaaata gcgtgattca gcgcgcttct tcgctggtct ttgacagtgt
    15721 ggaagccaaa aagcacgcga cgcgcaatcg cgccaatggc gagttgttct atggacggcg
    15781 cggaacgtta acccatttct ccttacaaca agcgatgtgt gaactggaag gtggcgcagg
    15841 ctgcgcgcta tttccctgcg gggcggcagc ggttgctaat tccattcttg cttttgtcga
    15901 acagggcgat catgtgttga tgaccaacac cgcctatgaa ccgagtcagg atttctgtag
    15961 caaaatcctc agcaaactgg gcgtaacgac atcgtggttt gatccgctga ttggtgccga
    16021 tatcgttaag catctgcagc caaacactaa aatcgtgttt ctggaatcgc caggctccat
    16081 caccatggaa gtccacgacg ttccggcgat tgttgccgcc gtacgcagtg tggtgccgga
    16141 tgccatcatt atgatcgaca acacctgggc agccggtgtg ctgtttaagg cgctggattt
    16201 tggcatcgat gtttctattc aagccgccac caaatatctg gttgggcatt cagatgcgat
    16261 gattggcact gccgtgtgca atgcccgttg ctgggagcag ctacgggaaa acgcctatct
    16321 gatgggccag atggtcgatg ccgataccgc ctatataacc agccgtggcc tgcgcacatt
    16381 aggtgtgcgt ttgcgtcaac atcatgaaag cagtctgaaa gtggctgaat ggctggcaga
    16441 acatccgcaa gtagcgcgag ttaaccaccc tgctctgcct ggcagtaaag gacacgaatt
    16501 ctggaaacga gactttacag gtagcagcgg gctattttcc tttgtgctta agaaaaaact
    16561 cagtaatgaa gagctggcga actatctgga taacttcagt ttattcagca tggcctactc
    16621 gtggggcggg tatgaatcgt tgatcctggc gaatcaacca gaacatatcg ccgccattcg
    16681 cccacaaggc gagatcgatt ttagcgggac cttgattcgc ctgcatattg gtctggaaga
    16741 tgtcgacgat ctgattgccg atctggacgc cggttttgcg cgaattgtat aacattgcca
    16801 cttttggaca attttgcaga catttaattg tgaaaagtct taaattgttg cgtccgggat
    16861 caaggcgtcc cggacgaatc aggagtacaa taggcagata aaggcttaaa cgctgttcca
    16921 caggaaagtc catggctgtt attcaagata tcatcgctgc gctctggcaa cacgactttg
    16981 ccgcgctggc ggatcctcat attgttagcg ttgtttactt tgtcatgttt gccacgctgt
    17041 ttttagaaaa cggcctgctg cccgcctcat ttttgccagg cgacagcttg ttgatactgg
    17101 caggcgcatt gattgcccag ggggttatgg attttctgcc tacgattgcg attctgaccg
    17161 ccgcagcaag tctgggctgc tggctaagtt atattcaggg gcgctggtta gggaatacca
    17221 aaacggtgaa aggctggctg gcacagcttc ctgctaaata tcaccagcgc gccacctgca
    17281 tgtttgaccg ccacggtctg ctggcgctgc tggctggacg ttttcttgca tttgtccgta
    17341 cgctgctgcc aaccatggcg ggaatttccg gtctgccaaa ccgccgcttc cagtttttca
    17401 actggttaag tggattgctg tgggtcagcg tggtaaccag ttttggctat gccttaagta
    17461 tgattccgtt cgttaaacgc catgaagatc aggtaatgac gttcctgatg atcctgccaa
    17521 ttgccttgtt aaccgctggc ttgttaggca cgctgtttgt ggtgattaaa aaaaaatact
    17581 gtaacgcctg acgattttcc ccgttcccgg ctgctgtacc gggaacgtat ttaattcccc
    17641 tgcatcgccc gcattcttgc cgcatcttcc cccggcgtca caccgaagta acgtttaaac
    17701 tcacggctaa attgcgatgc gctttcatag ccgacgcgca tcgctgctgc gctggccttc
    17761 atgccgtcat ggatgatcat catccgcgcc ttatgcagac ggtaattctt caaatactgc
    17821 aacggcgagg tgctggtgac agacttaaaa ttatggtgga acgccgatac gctcatgttg
    17881 gcttctgccg ccagttgctc gacgctcagg ttttcggtgt atttattctc aatccgtttc
    17941 agcacgcggc taatcagact gaagtgagtc tggcgactga ccagcgccag taacgcgccg
    18001 ccgcaaggtc cggtcagcac gtagtacaga atttcgcgga tgatctgttt gccgagaata
    18061 cgcgcatcca gtggtcgctc catcacgtcg agtaaccgct ccgccgcgca taaaatctct
    18121 tctgataacg tggcggagtt aatcccgctg gctgccatcg acggctggaa atgctcatct
    18181 tcgccaatgt ccatcaacag ttcctgtaac tgcaaaatat cgacattgag acgcaaccct
    18241 gccagcggca cctctgacgt ggcataggtt tcgcactcaa acggcaacgg caccgtcagc
    18301 agcaggtatt cattggcatc ataacgaaac acgcgttcat tgatataacc gattttatgc
    18361 ccggaaaaga gaattatgat gccaggctcg tacatcaccg gtgtacgtgc gaaaggtgtc
    18421 tcgccataca acaaacgcac atcgggcaac agttctgaca aactattttc tttatttttc
    18481 agtttattaa ctttatccgc cagcaagcgg caaatctctt cacgtttcat atcgcgtaat
    18541 ttcttaggaa taatgcggca atttgattgt gcgcaatttt gtagcatttc tccagcactc
    18601 tggagaaata ggcaagacat tggcagaaat gagcattgag agccagggcg ctggcgatca
    18661 caatgaaaaa catcaggcag atcgttctct gccctcatat tggcccagca aagggagcaa
    18721 gtaatgaaca actttaatct gcacacccca acccgcattc tgtttggtaa aggcgcaatc
    18781 gctggtttac gcgaacaaat tcctcacgat gctcgcgtat tgattaccta cggcggcggc
    18841 agcgtgaaaa aaaccggcgt tctcgatcaa gttctggatg ccctgaaagg catggacgtg
    18901 ctggaatttg gcggtattga gccaaacccg gcttatgaaa cgctgatgaa cgccgtgaaa
    18961 ctggttcgcg aacagaaagt gactttcctg ctggcggttg gcggcggttc tgtactggac
    19021 ggcaccaaat ttatcgccgc agcggctaac tatccggaaa atatcgatcc gtggcacatt
    19081 ctgcaaacgg gcggtaaaga gattaaaagc gccatcccga tgggctgtgt gctgacgctg
    19141 ccagcaaccg gttcagaatc caacgcaggc gcggtgatct cccgtaaaac cacaggcgac
    19201 aagcaggcgt tccattctgc ccatgttcag ccggtatttg ccgtgctcga tccggtttat
    19261 acctacaccc tgccgccgcg tcaggtggct aacggcgtag tggacgcctt tgtacacacc
    19321 gtggaacagt atgttaccaa accggttgat gccaaaattc aggaccgttt cgcagaaggc
    19381 attttgctga cgctaatcga agatggtccg aaagccctga aagagccaga aaactacgat
    19441 gtgcgcgcca acgtcatgtg ggcggcgact caggcgctga acggtttgat tggcgctggc
    19501 gtaccgcagg actgggcaac gcatatgctg ggccacgaac tgactgcgat gcacggtctg
    19561 gatcacgcgc aaacactggc tatcgtcctg cctgcactgt ggaatgaaaa acgcgatacc
    19621 aagcgcgcta agctgctgca atatgctgaa cgcgtctgga acatcactga aggttccgat
    19681 gatgagcgta ttgacgccgc gattgccgca acccgcaatt tctttgagca attaggcgtg
    19741 ccgacccacc tctccgacta cggtctggac ggcagctcca tcccggcttt gctgaaaaaa
    19801 ctggaagagc acggcatgac ccaactgggc gaaaatcatg acattacgtt ggatgtcagc
    19861 cgccgtatat acgaagccgc ccgctaagct ttttacgcct caaactttcg ttttcgggca
    19921 tttcgtccag acttaagttc acaacacctc accggagcct gctccggtga gttcatataa
    19981 aggaggaacg tatggctaat ccaaccgtta ttaagctaca ggatggcaat gtcatgcccc
    20041 agctgggact gggcgtctgg caagcaagta atgaggaagt aatcaccgcc attcaaaaag
    20101 cgttagaagt gggttatcgc tcgattgata ccgccgcggc ctacaagaac gaagaaggtg
    20161 tcggcaaagc cctgaaaaat gcctcagtca acagagaaga actgttcatc accactaagc
    20221 tgtggaacga cgaccacaag cgcccccgcg aagccctgct cgacagcctg aaaaaactcc
    20281 agcttgatta tatcgacctc tacttaatgc actggcccgt tcccgctatc gaccattatg
    20341 tcgaagcatg gaaaggcatg atcgaattgc aaaaagaggg attaatcaaa agcatcggcg
    20401 tgtgcaactt ccagatccat cacctgcaac gcctgattga tgaaactggc gtgacgcctg
    20461 tgataaacca gatcgaactt catccgctga tgcaacaacg ccagctacac gcctggaacg
    20521 cgacacacaa aatccagacc gaatcctgga gcccattagc gcaaggaggg aaaggcgttt
    20581 tcgatcagaa agtcattcgc gatctggcag ataaatacgg caaaaccccg gcgcagattg
    20641 ttatccgctg gcatctggat agcggcctgg tggtgatccc gaaatcggtc acaccttcac
    20701 gtattgccga aaactttgat gtctgggatt tccgtctcga caaagacgaa ctcggcgaaa
    20761 ttgcaaaact cgatcagggc aagcgtctcg gtcccgatcc tgaccagttc ggcggctaac
    20821 atgcaaattc tcccggtggc ggtaatgttc cgctaccgga cttttcagaa atcatttatt
    20881 cccctcgcgt cccgcccgtt gttactcttc cttgttcagg aatgccaaat ataaggacat
    20941 catcatgcag agccggaagc tcttaaaaga acaactcatc tatatccggg ataaacgcaa
    21001 cggagaggtg aaaaacagat gaaaataata cttctgtttt tagcagccct ggcaagtttt
    21061 accgtacacg cacagccccc ctcacagacc gtagaacaaa cagtccggca tatttatcag
    21121 aactataaat cagatgccac tgccccttat tttggtgaaa ccggagagcg ggcgataact
    21181 tctgcgcgta ttcaacaggc gcttaccctg aacgacaatc ttacgctgcc gggcaatatt
    21241 ggctggctgg attatgatcc ggtttgtgat tgtcaggatt ttggcgatct ggtgctagaa
    21301 agcgttgcga taacccaaac tgacgccgat catgccgatg ccgttgtgcg ctttcgtatc
    21361 tttaaagatg ataaagaaaa gaccacgcag acactgaaaa tggtggcgga aaatggtcgt
    21421 tgggtcattg acgatattgt cagcaatcat ggcagcgtct tacaagcagt taatagcgag
    21481 aatgaaaaaa cgctggccgc tttagcttcg ttgcaaaaag aacagccgga agcctttgtt
    21541 gccgaactct ttgaacatat tgctgattat agctggccgt ggacgtgggt ggtttccgac
    21601 tcttaccgcc aggcggttaa tgccttctat aaaaccacct tcaagacggc caataatccc
    21661 gatgaagata tgcaaataga acggcaattt atttacgaca atccgatctg ttttggcgaa
    21721 gagtcgctat tttcacgcgt tgatgaaatt cgagtcctgg agaaaaccgc cgattccgcc
    21781 cgcattcatg ttcgttttac gctgaccaat ggcaacaacg aagagcaaga actggtttta
    21841 cagcggcgcg aaggcaagtg ggaaatcgct gattttatcc gcccgaacag cggcagccta
    21901 cttaagcaga ttgaggcaaa aactgccgcc agattaaagc aatgagctga attaaataac
    21961 aattagccgg aacaataaat aaaagggaac actatatgaa aacgattttc accgtgggag
    22021 ctgttgttct ggcaacctgc ttgctcagtg gctgcgtcaa tgagcaaaag gtcaatcagc
    22081 tggcgagcaa tgtgcaaaca ttaaatgcca aaatcgcccg gcttgagcag gatatgaaag
    22141 cactacgccc acaaatctat gctgccaaat ccgaagctaa cagagccaat acgcgtcttg
    22201 atgctcagga ctattttgat tgcctgcgct gcttgcgtat gtacgcagaa tgataaaaaa
    22261 atccccggta gcgtgtcagt tgccggggat attttttaac gtccaaccgc cgctttaggc
    22321 cgtttcttcg caccagcatt caccggacga gattgcgtag acgacgcttt ttttgccgta
    22381 gcaggcgtct gacgctgggt cgccatcggc gtatgtttcg tcaacgccgg acgggtattg
    22441 cggttctggc gacgagcttc acgcatctct tcaatggttg gcgcaggcac taagcaatcg
    22501 cgacggctgc caatcagatg ctttttgccc atcgcttcca gcgcctggcg gattaacggc
    22561 cagtttgccg gatcgtggta acgcaacaac gctttatgca aacgacgctg tttgtcgccc
    22621 ttcggtacga agacgtcttc gctcttataa ccaatcttcg ccagcgggtt tttcccggtg
    22681 taatacatgg tggttgagtt cgccagcggc gacggataga agttctgcac ctggtcgagg
    22741 cggaagcggt gctttttcag ccacagcgcc agattcacca tatcttcatc acgcgtaccg
    22801 gggtgcgcgg agatgaaata ggggatcaga tactgctctt tacctgcctg ttttgagtaa
    22861 gtatcgaaca gctctttaaa gcggtcatag ctgcccatgc ccggcttcat catcttcgat
    22921 aacggccctt cttcggtatg ttccggggca atcttcagat aaccgccgac gtgatgggtc
    22981 gccagttctt tgatatagcg cggatcttct acggctatgt cataacgcac accagaggca
    23041 atcaggatct ttttaatgcc tttcagatca cgcgcacggc gatagaggtt gatcgttggt
    23101 tcgtggttcg tgtccatgtg cggacaaata tccggataaa cgcacgacaa acggcgacaa
    23161 gtttgttcag cgcgtggcga tttgcagcgc aacatataca tgttggcagt tggcccacca
    23221 agatcggaaa tcacgcccgt aaaacctgga acggtgtcgc ggatcgcttc gatctcatta
    23281 atgatcgaat cttcggaacg gctctgaata atgcgccctt cgtgctcggt gatagaacag
    23341 aaagagcagc cgccaaagca gccacgcata atgttgaccg aaaaacggat catttcgtaa
    23401 gccggaatac gggcattgcc ataggccgga tgtggcacgc gcttgtatgg cagcgcaaaa
    23461 acgctgtcca tctcttcggt agaaagcgga atagcaggcg ggttgatcca cacatagcgg
    23521 tcgccgtgtt tttgcatcaa tgcgcgggca cagcctgggt tggtttcgtg gtgcagaata
    23581 cgcgaagcat gggcgtacag cactttatcg cccttcactt tctcgaaaga aggcagcaac
    23641 acgtaggttt tttcccacgg tttcgggcgc ggtggctgca cggttacggc tttggcttcc
    23701 tgctttttcg gtgccaccgg tttgttatcc gcgcacggca aatcttcacc atacggatgc
    23761 gggattgggt cgatttttcc aggggtatca agacgggtgg aatccacgcc gctccagcca
    23821 ggcagcgcct ctttcacgat aatcgcggta ttacgcacat cgcggatttc actaatcggc
    23881 tcgcccatcg ccagacggtg cgccacctcc accagcggac gctcaccgtt accaaacatc
    23941 agcatgtcgg ctttcgaatc caccagcacg gaacggcgca cggtatcgga ccagtaatca
    24001 taatgtgcgg tacggcgcag actagcctca ataccgccga ggatcaccgg tacatctttc
    24061 cacgcctctt tacaacgctg ggtataaacc agtgtggcgc gatccgggcg cttacccgcg
    24121 acgttatccg gcgtgtaggc atcgtcatga cgtaaacggc gatcggcggt ataacggttg
    24181 atcatcgaat ccatgttgcc agcagtaaca ccgaaaaaca gattcggttt acccagacgc
    24241 atgaaatcgt ctttgctgct ccagtctggc tgggcgatga tcccgacgcg aaagccctgt
    24301 gcttccagca tacgaccgca aatcgccatc ccgaagcttg ggtgatcgac atacgcgtcg
    24361 ccagtaacca aaatgatgtc gcagctatcc cagccaagtt gatccatctc ttcacgagac
    24421 atcggcaaaa acggtgccgg tccaaaacag gcggcccagt actgcggcca ggagaacagg
    24481 tcgcgatccg gttggatcag ggagatagag ctcattttgc ttccagaaat gataaaaaaa
    24541 taatcaaagg ccggggatta taagccggaa cgaaagagaa atcgaaaagt attccatacc
    24601 cgccctcctc gggcgagtat gaagattacg gtaccggatt gactaacagt tgcccaatcg
    24661 agccacggtc cgccatttcc agcgtctgac tgttgaagta gaacgggaag tgcgcccagg
    24721 aaggctgacc gaaataaaca agcagctcca cttgtccgtc aacccaaacg gtatctttcc
    24781 agcctctgtc ttccgggaac ggcatcgcgc cattcacgtt acggatctgg aacattacgc
    24841 cttcaatatg gaacgcttgc ggctcgtccg cgcgtaccgt ccagcgttcc cacgttcctt
    24901 gctgcgcggt gacatcaata cggttgacgt cccacagctg tccattaata cccgggtcat
    24961 cacccagact gatatcgcga ctgcgaattg gcgaaccagc catgatttca gttggcagca
    25021 agcgcatcgg aagactgtct gtgaccagcg gcagaaggcc ggttgggcgc agcgttagca
    25081 ccagggtaga aaccagaata ctggatggct caaagaagcc acgaatacga tcaacaatgc
    25141 tcgccgcttc gccacaggtg atcgacactt catcgccgtt gctcatatcc accagaatct
    25201 cgcggcgctc gcccggtgcc agcgaaagtt gcttcaccga tacaggagca ggcaggaatc
    25261 cctgatcgcc agaaatcaca tgtaacgggc gaccatcgtt catctgtagt tgatagcgac
    25321 gagagttcga cgcgttcagc agtcgcaagc gcacccagcc acgcgagact tcaacgtacg
    25381 ggctttgtac accgttaacc agcagcgtat caccaacaaa gccgccgctt cccggttcgt
    25441 tgtattctgg cgtaccaaag ttatccagcc gtttatcctg gataatgacc ggaaaatcat
    25501 ccacaccata atggttgggg ataggcagcg acttgctgac ttcatcttcc accagccaca
    25561 ttccggcaag gccgttatag acctgctgag ccgtgcggtt gggagtattg gcgtgatacc
    25621 acagagtagc tgcgttctgg cgaatgggca gtacgggtgc ccagtcagcg tttggcgaca
    25681 tcatccgtgc cggaccgccc atcagcgggc ctggtacctg tagcccggcc accgtcattg
    25741 agacattttc tgtcaggcgg ttgctgtaaa taagcttaac atcgtcgccc ttccagacgc
    25801 ggatagtcgg ccccaggtaa cgaccattga ttccccagac cgacgcgcgt gtccctggcg
    25861 taaatgacca gtgcgcacgt tgtacagtca taaacagcgg ttgcccacgg cgagattcaa
    25921 gtagcggcgg aacgggtagc ggttgctgtt gcccggctgc gctggccttc aggggaacag
    25981 cgcctgcaca aagtgcaatc cccgatgcct gaatgaactg acgccgactg agtgacatat
    26041 ttgctccatg taaaactggc taaagtaata ccgtgcagga aagtgttccc cgcaaaaata
    26101 cagattgccg acttaaactt ttccggcggc ttcgcgttct gcgacttctt tatcgagctc
    26161 ggcgattttt tgttccatta tcgaacgaca atgggcagcc agctcacgaa cctgatcttt
    26221 gccatactga ctgacgtcaa ttggcggcag catttcgaca atcaccagac cgttgtgcag
    26281 tcgattaaga ttaatcttat tcgaagttgt agagacgcac acgggaataa tcgggacgcc
    26341 cgccgcaatt gccgcgtgaa atgctccagt cttgaacggt agcaggccgc gaccacggct
    26401 gcgggttcct tccgggaaca tccagatgga aatacggcgt tttttgaagt gattcactac
    26461 ttccgcaatg gtgccgtgag ctttagtgcg attgtttctg tcgatcaata agttgccggt
    26521 taaccagtac aactgcccga agaaggggat ccacagcaag ctctttttac ctaccgtcac
    26581 cgtcggcggt tgcacgatgt tcgatgctgt caccatgtca tagttgttct ggtggttagc
    26641 gatatagata gcattgccgt agctttcagc gtctgtaggt ttacggcact caactttcag
    26701 gccaaacagc ggcgcaagac ggccaaacat atgcccaaag gtggccacat gtttcgggtt
    26761 acgcgggctg aaaaggcagt aaatggagcc gaatacacag actaagatgc tgtaaatcac
    26821 ggtaataata agacgaaaga tatatagcat aacaccctct gaaggtctga gagactggca
    26881 ttgtacgtca cctgaaatca ggttagggca ttgttaaaag ccgctctctg gaaaagcgta
    26941 ttgttaatcg caacgcatga ataaacaacg gtttcaaggg aatttttatc gtttccgggg
    27001 gttgaatacg ctgccggatg acgacttaac gtttcatccg gcgttccttg caagcgggag
    27061 gaaacagcgc cctccccggc atattactct tcgctatcac cgctgctggc acggcgagga
    27121 gagtcgatct caacacgatc gatacgctgc aaaccgcgca tcaacgtacc gcggcgtcca
    27181 cgttcgccag tgactttctg taactcttcc gggcgcagtt taattttgcg tttcccaaca
    27241 tgaatggtca gcgtgctttg cggcggcaga acgtacaatt gcgccagacc atcttctcca
    27301 cgcgcggctt ctgccgatgg aatgttgata atcttgttgc ctttgccctt cgacagctgc
    27361 ggcagatcac ttaccgggaa catcaacata cggcctgcct gagtgattgc cagcagcata
    27421 tcggaagcat cttcaatcac caccggcggc ataacatggg cattttccgg taaggtgatc
    27481 aaagccttac ctgcacggtt acgcgccacc agatcgttaa aggtgcagac gaaaccgtaa
    27541 cccgcatcgg aagccatcag cagtttctga tcgtcgcttt ccatcagcat atggtcaacg
    27601 gtcgccccag gcggcaacgt taatttgccg gtgagcggct cgccctgacc acgcgccgac
    27661 ggcagcgtaa tcgggtcaat ggcatagcta cgaccggtgg aatcaacaaa cactaccggt
    27721 tggttgctct tacctttcac cgccgctttg aagctatcac ccgctttata attcaggccc
    27781 ggcgcgtcga tatcatggcc tttagcgctg cgtacccagc ccatctgcga cagcacaatg
    27841 gtgacaggtt cagacggcag catgtcgtgc tcgctcatcg ctttcgcttc ttcgcgttcc
    27901 tgcaacggcg aacgacgatc gtcaccgtag gcttgcgcgt ctgcctgcag ttctttcttc
    27961 agcaggttat tcattttacg ctcggaagcc aaaatgccct gcaactggtc gcgctctttt
    28021 tccagttcac tctgctcacc gcgaatcttc atctcttcca gtttggcaag atgacgcagt
    28081 ttcagttcga ggatcgcttc cgcctgggtt tccgtaaggc caaaccgcga catcagcgcc
    28141 ggtttcggtt catcttcatt acgaatgatc tcaatcactt cgtcgatatt gagaaacgcc
    28201 accagcaaac cttcgaggat atgcaggcgc ttgaggactt tctccagacg atagttcagt
    28261 cggcggcgca cggtatcgcg gcggaacacc agccattcgg agaggatttc cagcaggttt
    28321 ttcaccgccg gacgaccatc cagaccgatc atattaaggt taatacgata gctcttttcc
    28381 agatcggtgg tagcgaagag gtggttcatc acctgatcca tatccacgcg gttggaacgc
    28441 ggcacaatca ccaggcgggt cgggttctcg tggtcagatt catcgcgcag atcgtcaacc
    28501 atcggcagct ttttgttgcg catttgcgca gcaatttgct ccagtacgcg cgcacctgaa
    28561 acctgatgcg gcaatgcgct gataaccacc gcgccatctt ctttcttcca caccgcgcgc
    28621 atacgcactg aaccacgtcc gttctcgtag attttacgga tctcggcgcg cgaagtgata
    28681 atttccgctt cagtcggata atccggcccc tgcacgatat ccagcagctg atcgagcgtg
    28741 gttttcggct ggtcgattaa tgcgattgcc gcctgagcca cttcacgcag gttatgcggt
    28801 ggaatatcgg tcgccatgcc gacggcaata ccggtggtgc cgttaagcaa aatgtttggc
    28861 agacgggcag gtagcatttt cggctcctgc aaagtgccgt cgaagtttgg cacccagtca
    28921 gccgtcccct gccccagctc gctcaatagc agctcggaat atttcgacaa ccgggattcg
    28981 gtgtaacgca ttgccgcgaa cgatttcgga tcgtccggcg cgccccagtt cccctgacca
    29041 tcaaccagcg gataacggta agagaacggt tgcgccatca ggaccatcgc ttcataacag
    29101 gcgctatcgc cgtgcggatg gtatttaccc agtacgtcac cgacggtacg ggccgatttt
    29161 ttaaatttgg cgctggcatt caggcccagt tcagacatcg catacacaat gcggcgctga
    29221 acaggtttca gaccatcacc aataaacggc aacgcacggt ccatgatcac gtacatggag
    29281 tagtttaagt aggcgttttc cgtaaattca tgtagcgcaa ggcgctctgc catatcgctc
    29341 attaattctg attcctcaac ttattcgcca gccttcaaag gggcaatatt gccggagata
    29401 ctacattatc tgccgcgtcg agtcacagga gatcagcgtg aatagcgccg ccggacgtgc
    29461 tccgacggcg ataaaggcat caatgtgcct tgatatacaa ctctttgctg tagacataat
    29521 cctggggatt atgcagcgga aaaccgccaa cgtagggttt aagcagtttg attaacggct
    29581 gatagtagat aggaatcagc ggtgcctgtt ggttgatgat cacttctgcc tgctgataca
    29641 acgcattacg ctttgtcgca tcagtgatct gcgtggcctg gtttagtaag gcgtcatact
    29701 gcgcattttt ccagtgaccg acgttttctt cactatcgct tttgagcgtg ttcaggaagc
    29761 tggaagcatc attgtacgtc gcatcccacg actgccgaga cagcatgaaa tcaccggctc
    29821 gtcgggcatc aagataggtt ttccactcca ttgtgcgcag cgtcacctgt gcacccagcc
    29881 attttttcca ttcggaagac aacgctatcg cggtcttttc atgcagatcg tacttgttgt
    29941 agaacagctc aaagcgtagc ggatgagagg cgtcgtatcc cgcctgtttc agcaaggctt
    30001 ttgccatcgc gacgcgctca ctcattggct tttgcagttc atcgaacgtc gtcgcgctaa
    30061 agccttttac ctctggcggc gtcagcgtgg ttgcgggcgt tctcaacccc agtacctttt
    30121 gcgcaataag ctgtcgatca accgtaagat atagcgcccg acgcactcgc acatcgttaa
    30181 atggcggttt ctcaaggttg aagttgtaat attcgctgtt cagacgcgga ataattcgta
    30241 gctcgccagg cagtgatttt tcaatggcgg gaatttgctg cgccggaacc caggtgagat
    30301 cgacctctcc cgcgcgatag cggttatagc cggtgaccga attatctagc gccagatact
    30361 caacctgttg caatactgta tgttgcgcat cgcggtactt tggatttttg cgtgcagtaa
    30421 tcttttcgtt aactacccac tgatcaagca caaaggcacc gttgtaaacc atgttctctg
    30481 gcttactcca gctatcgcca tgtttagcga tgacatgatg aggaaccggg aacagcgtcg
    30541 gccaggcgag catcgtcgtg aaccaaggaa ccggctgctc aagcgtaact tcaagagtac
    30601 gatcatccgt cgctttgaca cccagcgatg taacatctgc tttacccgca acaatagctg
    30661 cggcattgtt aatgtgcgcc tgtgccagat agccagcaaa agggcttgcc gttttcgggt
    30721 caaccgcgcg ctgccagccg aggacaaaat cctctgccgt cagaggctga ccgtcggacc
    30781 actgcaaacc gctacgcaga tggaaaatat agcgcttgcc gccgtccagt atctcccagc
    30841 gttcagcctg agcggactgc acctggcctt caccgtccat ccataccaga ccttcaaaca
    30901 gatccagcac aatctgcgcg gcagtattct cctccacctt ttgcgggtcg agcgtacctg
    30961 ggtcgctatg attgttgtaa cgaaagactt gttgcggggc gagcggtgtg ttggcgggaa
    31021 cgtcagcagc gtagagagga gccgcactta ccaggctgac cagccataat aaatttcgcg
    31081 tatacatggt attccttacc ggagaaatta taagcccaac aacaatacgc ctgtggcatt
    31141 gttcgctcaa acttatcgcg agtgatttgg ctcacactcc ggtaaagaaa aaagcgagtt
    31201 gcccccgctt ttccattaat taacggattt cattcaatag ttctggatgc ttatccagaa
    31261 cacgtaaaag tttgattgtg gaaggatgag gttgggcatt gcctttttcg taacgcgaaa
    31321 acgcatttac acctccccca aaaatttcgc ttgcctcttt ttgggtaaga gagagctttt
    31381 ttcgaacctt cactataaat tcaggtgcca ctgtttcggc attcaccgaa gcccgaaatg
    31441 cctttacttg cgccatgaaa gcatctgact cttctttatt catgatgctc tcttcgcaat
    31501 ggacacaata taaaccgtgg atacctttca atactttttt tcgtccacgg aaggtgtatg
    31561 gaatatcttt aatgccagaa accatttctc cctggtggca aaccggacat ttcatattac
    31621 ttctccttaa acgagacgat cagtacgtca tgaattaccg taattttaag ataaacctgg
    31681 cctgtaacaa gcctgggtct gtaaacatcc tgccagatag tatgatcaga gtaggtggtc
    31741 atgcttttat aaaagtcgct ctctgataat ccaatgataa cattacacat accatcaaaa
    31801 tccaaaccta actcatctgc atttaacagg gcactacgtg ttgtacgaac ttgcccggca
    31861 ttgacaagtt ttttaacctg actcaaacgt gtatgtggtg tgcgtttttc cataaccccc
    31921 gcctccctgt tactttagtt ataacctaaa aggttaatta cagcaatgaa aaagcaccta
    31981 aaaggttagt tagatgtacg gagatagtga ccacacaaaa cgtattcttt aaggaaagtg
    32041 attgaccata taagaaagtg gcgcattagt agcgccagtt tgaagcagga atttataagg
    32101 gaagctggag tcaggcatca cgccaacggc acataaacat ccgtttccag tcgctgctct
    32161 gtcacccctt cggcaagatt ggtgtagtga aacagaatcg gtgctttacg cattttctcg
    32221 ccgcttgcag gcagccagtg gcgaatgatg ccccataccg tgtgtgaaat atcgtccagc
    32281 tcgccaacgt ggcgggccac ggcataacgt ccaccggtaa gttcaccatt gctcacacca
    32341 taacgattat cgggaatcgg ttcgctaacg ctgccgcaga tatcaaagcg aaacgcttcc
    32401 ggtgcggtgg tggcagggtc gtcccaggcg acgccaaaag tctggctttg gttaacagga
    32461 gaaagccccg tctctttacg ccacatgata aatttcgcca cgctgtagtt gagcaattca
    32521 gggctacagc ggtgcggcaa catcgccaca ggtattgatg ggaaatcgat aatgtttaca
    32581 tccagtgtca ggtttgtcat tttgcctccc tgagaaatta ttcccgggaa cgcgtcccgg
    32641 gagcggtaac aattacggat ttactttgcg gatctgtttg acgtcaattt caacagaatt
    32701 ccagtcttta tcgacttcac cctgaatctc aaccgtatct ttcggcgtca ccgtcacgcc
    32761 gttccagcgt ttgtggtcga tatcaacatt gatagtaccg ctggcatctt tgaacacgta
    32821 gagatcgtca gagatgcgtt caacgatatt gccgcgcagg gttacccagg tgtcgtcacg
    32881 cagggatttt gcgctttcta cagtcgttac gctgccgttc ggcccctgga atcctccggc
    32941 ctgactttgc gttgccgatg ggccagaaaa accgccctgc tctgctgcca tcaccggtgc
    33001 gctgcacagg gccattactg cgattactgc tgcgaatttt ttcatgttta ttactccctt
    33061 taatgtctgt ttccgagcat ttaacaagat agtccttaac aacttcttaa gggaaaaaaa
    33121 taaaatttag tgctgtacag agcgcgttac aacacggttt actggcagca aatacggtta
    33181 tcgcagggat gaaaaaatgc gaattttact gatagaagat gacatgctga ttggcgacgg
    33241 catcaaaacg ggccttagta aaatgggttt tagcgtcgac tggtttacac aaggtcgtca
    33301 gggaaaagag gcgctttata gcgcacctta tgatgcggtg atcctggatt taaccttacc
    33361 aggcatggat ggtcgcgata ttttgcgcga atggcgagaa aaaggtcagc gtgagccggt
    33421 actgatcctg accgcgcgcg atgcgctggc ggaacgtgta gaagggctgc gtctgggagc
    33481 tgacgattat ctgtgtaaac cttttgcgtt gatagaagtc gccgccaggc tggaagctct
    33541 gatgcgccga accaacggcc aggccagcaa cgagctgcgc cacggtaacg tcatgctcga
    33601 ccccggcaaa cgtatcgcca cgctggctgg cgaaccctta acactgaaac caaaagaatt
    33661 tgccctgctg gaattactga tgcgtaacgc tggtcgggta ctgccgcgca aactgattga
    33721 agagaaactg tatacctggg acgaagaggt caccagtaat gccgttgaag tgcatgtgca
    33781 tcatctgcga cgcaaactcg gtagtgattt tattcgtacc gtgcatggta ttggctacac
    33841 attaggtgag aaatgaaatt tacccaacgt cttagtctgc gcgtcaggct gacgctaatc
    33901 tttttaattc tggcctcggt gacctggctg ctttccagct ttgtcgcctg gaaacaaaca
    33961 acggataacg tcgatgaatt gttcgacacc caactgatgc tgtttgccaa gcggttaagt
    34021 acgctcgatc tcaacgaaat caacgcggcg gatcgcatgg cacagacgcc aaatagatta
    34081 aaacacggtc atgttgatga cgatgcgctg acctttgcca tctttaccca cgacggcaga
    34141 atggtcctta atgatggcga taacggagaa gatattccct atagctatca acgggaaggt
    34201 tttgctgacg ggcaactggt cggtgaagac gatccttggc gttttgtctg gatgacctca
    34261 cctgatggca aatatcgcat cgttgttggc caggaatggg aataccgtga agacatggcg
    34321 ctggcgattg ttgccgggca attgatcccg tggctggtcg cactgccgat tatgttaatc
    34381 atcatgatgg tactactggg tcgtgaactc gcgccgctga acaaactggc gctggcacta
    34441 cgtatgcgtg accctgactc ggaaaaacca ctaaacgcga ctggcgtacc cagcgaagtg
    34501 cggccactgg ttgagtcgct aaatcaactg ttcgcccgca cacatgcgat gatggttcgt
    34561 gaacgacgct ttacctccga cgcagctcac gaacttcgta gcccgttaac ggcgctgaaa
    34621 gtgcaaaccg aagttgcgca gatctctgac gatgatccgc aggcgcggaa aaaagcactg
    34681 ctccaattac attccgggat cgatcgcgct actcgtctgg ttgatcaact gctcacgcta
    34741 tcgcggctgg actcactgga taaccttcag gacgtcgcgg agatcccgct tgaagatctc
    34801 ctgcaatcgt cggtgatgga tatttaccac acggcgcagc aggcgaaaat tgacgtgcga
    34861 ctgacactca atgcccacag catcaaacgc accgggcaac cgctattgct aagtttgttg
    34921 gtgcgaaatt tgctggataa cgccgtgcgc tacagtccac agggcagcgt ggtagacgtc
    34981 acgctgaatg ctgataattt catcgtgagg gataacggcc ccggtgtgac accagaggca
    35041 ctggcgcgaa ttggcgaacg cttctatcgc ccacccggac aaaccgctac cggcagcggg
    35101 cttgggctat cgattgtcca gcgaatcgcc aaattgcatg gcatgaatgt tgaatttggg
    35161 aatgcggaac aaggtggatt tgaggcgaag gtaagctggt aacttctctg tctatgagag
    35221 ccgttaaaac gactctcata gattttacta atagcaaaat ataaaccgtc cccaaaaaag
    35281 ccaccaacca caaaccagac aaataccgag cgagaataca tatacccaaa actcgaacat
    35341 ttcccgcata aagagttttc ttaagataag aataataagt ggcgtaagaa gaaaaaatgc
    35401 tgcaatcatt gcttgcaata tacgatcttc gcctgaaacg gaaggta
//
LOCUS       DAPHBJ010000025        34835 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.025, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000025 DAPHBJ010000000
VERSION     DAPHBJ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.025"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            27..620
                     /locus_tag="RHZ76_001140"
     CDS             27..620
                     /locus_tag="RHZ76_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2811813.1"
                     /translation="MKKTLLAAALVMVSGSALAVDGGHIDFNGLVQSGTCKVGVVDAG
                     MHGITTDGVVTLDTANVTDNFAEVNATAVGLLPKEFMISVNCEPGKTEASLTMGSASY
                     ANTSGTLNNNMNITVNGIQPAENVNIAVHNMTNKSGAVEIKQVHMNNSAEKHLLTLDA
                     NGDGQYVFNASYVKAPNSQDVTSGHVTTNALYTITYN"
     gene            703..1428
                     /locus_tag="RHZ76_001141"
     CDS             703..1428
                     /locus_tag="RHZ76_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDV2811814.1"
                     /translation="MFPIIKKTVSALFISTLLTSAPAFADIIISGTRIIYNADKKDVN
                     VRLENKGNRPLLIQNWLDTGDDNADPSQIKVPFTSTPPVSRIESKRGQTVKVMFTGTT
                     QLPADRESVFWFNVLEVPPKPKDAESDKNLLQLAFRTRIKLFYRPSGLKGEPAEAPAK
                     LTWKLNNAQLQVNNPTPYYVSFNDVAIESGGKSYKVDSSMVAPFGQVSFAVTDIASSV
                     SSGKVIYKAINDYGGNIDGSTSL"
     gene            1469..4069
                     /locus_tag="RHZ76_001142"
     CDS             1469..4069
                     /locus_tag="RHZ76_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HDV2811815.1"
                     /translation="MYTLTHQKSRLPKTTLLAACCAFFYSSNGAATESVEYDSSFLMG
                     TGASSIDVKRYSQGNPTPPGVYNVRVFINQQSVASLELPFVDIGENSAAACITLKNLA
                     QLHIKKPEYPITLLAREGEEGDCLDIKKSIEQAEVSYDGSEQHLEISVPQAYVYKTYG
                     GYVDPSLWESGINAAMLSYSLNAYHSDSKNGNRDSIYGAFNTGLNLGAWHLRARGNYN
                     WSQDNGNSFDFQDRYLQRDIPAIRSQVIVGDAYTTGETFDSVNVRGMRLYSDSRMLPS
                     ALASYAPIIRGVANSNAKVTVTQNGYKIYESTVPPGEFVIDDLSPSGFGSELVITIEE
                     ADGSKRSFTQPFSSVVQLQRPGVGRWDISAGQIIDDSLRHEPNMAQASFYYGFNNLFT
                     GYTGVQITDNDYMSGLLGLGINTSIGAFAVDITHARTEIPDDKTYQGQSYRITWNKLI
                     EATSTSFNLAAYRYSTEDYLGLHDALALIDDANHLSVNDDKDTIRTYSRMKNQFTVSV
                     NQPLSFAYEDYGSLFLSGSWTNYWAGNNNRTEYNVGYSKSVSWGNFSVNLQRSWNEDG
                     NKDDAMYLNVSVPLENIFGGKRKSSGFRNLSTQFNTDFNGSHQLNVSSSGNSEDNLIG
                     YSVNTGYNLDKESENVASVGGYLSYDSTWGGFSASASASTDNRQQYSVSTDGGFVLHS
                     GGLTFTNNSFGSNDTLVVIKAPGAKGARVNNGTDEIDRWGYAVSSSLSPYRENRVGLN
                     IETLENDVELKSTSATTVPRSGSIILASFETDQGRSAVLNISTSNGKPIPFAAEVYQD
                     EIMIGSMGQGGQAFVRGINDSGELIIRWFEDSRTINCKLHYQLPAQPETLGSTNTLLL
                     NNLTCKLVNH"
     gene            4083..4640
                     /locus_tag="RHZ76_001143"
     CDS             4083..4640
                     /locus_tag="RHZ76_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2811816.1"
                     /translation="MKRIIIISTLVGLGLPTTGSATNINVDFTAQLLATTCTITIKED
                     GGPAVNNTGGDDYSMTIPTVGLDKIANAHADAQANFQLVAKDCSEGYSKIITKLSGAT
                     ISGNLIKNEATDTPVATNIGMGIKRRDAADASFITPNNSASVEWTTEDKTNGMKLTVA
                     LRETTAGTGNIGNFRAKATFNFTYE"
     gene            4655..5250
                     /locus_tag="RHZ76_001144"
                     /pseudo
     CDS             4655..5250
                     /locus_tag="RHZ76_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241844.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            5271..5861
                     /locus_tag="RHZ76_001145"
     CDS             5271..5861
                     /locus_tag="RHZ76_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2811817.1"
                     /translation="MLFQEHNFALFFGLVTALCSPVSLANKDVDLTANIVSSTCQIEI
                     NNNGVVDLGTVTLDYFSNNITPDTDYAGGKNFTVNVVSCDNLQTTQSQIKFDFQPQTG
                     SLAQANQQVFSNQYELQSIGAKNVGVVIFSAQPHAQKFNVRGTDGASKAIYALPSNQV
                     VPSTWTFYARMQKVNNALAPVAGIVRSQVIVNVYYE"
     gene            5873..6985
                     /locus_tag="RHZ76_001146"
     CDS             5873..6985
                     /locus_tag="RHZ76_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001489301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2811818.1"
                     /translation="MQGKVKKILLHIYSAVLLFLASSWYVFAGTGSECYIESSSAGSY
                     TIFINTPPYSVATQQVTTPYEISDAATDVPIVLRGDGIACKAIPNGDESIHFLNTADA
                     NLISTYTSARGSTLLKTTVPGIAYTVELICDTCPEVVDLRIPPAGDDNFTPNSDIWWV
                     WGETDEKWKLRFRFFYTPEFKPKNGVTSGTLLPGKIASWYIGINTQPWINFYVDADTF
                     KFTVDQPTCSTIALDSTSQNISGNKINLGDYYVSQVKQEITQVVPFTIRGDYCYASKI
                     TVKLKATTEAPNKKLIGKSSGSATGVGVKVYSTANNSEVQLNADNSNEIIFNYANWSN
                     NLLYFPFTAQLVKDGSNGTISPGDFTGNATFSFSYE"
     gene            7119..7913
                     /gene="panB"
                     /locus_tag="RHZ76_001147"
     CDS             7119..7913
                     /gene="panB"
                     /locus_tag="RHZ76_001147"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDV2811819.1"
                     /translation="MKPTTISLLQKYKQEKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            7925..8776
                     /gene="panC"
                     /locus_tag="RHZ76_001148"
     CDS             7925..8776
                     /gene="panC"
                     /locus_tag="RHZ76_001148"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDV2811820.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(8850..9752)
                     /gene="rpnC"
                     /locus_tag="RHZ76_001149"
     CDS             complement(8850..9752)
                     /gene="rpnC"
                     /locus_tag="RHZ76_001149"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HDV2811821.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEVSQEFAQRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            10026..10406
                     /gene="panD"
                     /locus_tag="RHZ76_001150"
     CDS             10026..10406
                     /gene="panD"
                     /locus_tag="RHZ76_001150"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDV2811822.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10410..11639)
                     /locus_tag="RHZ76_001151"
     CDS             complement(10410..11639)
                     /locus_tag="RHZ76_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDV2811823.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNASSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLSVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDNAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(11703..12143)
                     /locus_tag="RHZ76_001152"
     CDS             complement(11703..12143)
                     /locus_tag="RHZ76_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV2811824.1"
                     /translation="MLGWVITCHDDRAQEMLDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRKTMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12248..13018)
                     /locus_tag="RHZ76_001153"
     CDS             complement(12248..13018)
                     /locus_tag="RHZ76_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2811825.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13015..13941)
                     /locus_tag="RHZ76_001154"
     CDS             complement(13015..13941)
                     /locus_tag="RHZ76_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2811826.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14050..14712
                     /gene="can"
                     /locus_tag="RHZ76_001155"
     CDS             14050..14712
                     /gene="can"
                     /locus_tag="RHZ76_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HDV2811827.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(14753..15289)
                     /gene="hpt"
                     /locus_tag="RHZ76_001156"
     CDS             complement(14753..15289)
                     /gene="hpt"
                     /locus_tag="RHZ76_001156"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDV2811828.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15495..17885
                     /gene="gcd"
                     /locus_tag="RHZ76_001157"
     CDS             15495..17885
                     /gene="gcd"
                     /locus_tag="RHZ76_001157"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HDV2811829.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18087..19637)
                     /gene="cueO"
                     /locus_tag="RHZ76_001158"
     CDS             complement(18087..19637)
                     /gene="cueO"
                     /locus_tag="RHZ76_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HDV2811830.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            19803..20150
                     /locus_tag="RHZ76_001159"
     CDS             19803..20150
                     /locus_tag="RHZ76_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HDV2811831.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20256..21122
                     /gene="speE"
                     /locus_tag="RHZ76_001160"
     CDS             20256..21122
                     /gene="speE"
                     /locus_tag="RHZ76_001160"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDV2811832.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNSAIHTAAFALP
                     QYLQDALASQPS"
     gene            21138..21932
                     /gene="speD"
                     /locus_tag="RHZ76_001161"
     CDS             21138..21932
                     /gene="speD"
                     /locus_tag="RHZ76_001161"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDV2811833.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(21970..22332)
                     /locus_tag="RHZ76_001162"
     CDS             complement(21970..22332)
                     /locus_tag="RHZ76_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HDV2811834.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22507..25104)
                     /gene="acnB"
                     /locus_tag="RHZ76_001163"
     CDS             complement(22507..25104)
                     /gene="acnB"
                     /locus_tag="RHZ76_001163"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HDV2811835.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25459..27312
                     /locus_tag="RHZ76_001164"
     CDS             25459..27312
                     /locus_tag="RHZ76_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HDV2811836.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKTVPVKQTVTAPVIPSN
                     TVSTATPVITEPATTVISIEPGNPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLIQQQKDAARQRYQSASPEQRQAVRERMQTNPQIQQRREAARERIQSASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTARLNNEQRSAVRERLSERGARRLER"
     gene            complement(27383..28807)
                     /gene="lpdA"
                     /locus_tag="RHZ76_001165"
     CDS             complement(27383..28807)
                     /gene="lpdA"
                     /locus_tag="RHZ76_001165"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDV2811837.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29132..31060)
                     /gene="aceF"
                     /locus_tag="RHZ76_001166"
     CDS             complement(29132..31060)
                     /gene="aceF"
                     /locus_tag="RHZ76_001166"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDV2811838.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAE
                     NDAYVHATPLIRRLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATG
                     GGIPGMLPWPKVDFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELE
                     AFRKQQNEEAAKRKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINI
                     GVAVDTPNGLVVPVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIG
                     GLGTTHFAPIVNAPEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGA
                     RFITIINNTLSDIRRLVM"
     gene            complement(31075..33738)
                     /gene="aceE"
                     /locus_tag="RHZ76_001167"
     CDS             complement(31075..33738)
                     /gene="aceE"
                     /locus_tag="RHZ76_001167"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HDV2811839.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIEQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(33899..34663)
                     /gene="pdhR"
                     /locus_tag="RHZ76_001168"
     CDS             complement(33899..34663)
                     /gene="pdhR"
                     /locus_tag="RHZ76_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HDV2811840.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         8705 a         9101 c         8392 g         8637 t
ORIGIN      
        1 ataacttttt tgtaaggaca catgtaatga aaaaaacatt actcgccgcc gcactggtga
       61 tggtttctgg ttccgctttg gctgtagatg gtggtcatat tgattttaac ggtctggttc
      121 agtctggtac ttgtaaagtc ggcgtggtag acgcgggtat gcacggcatt actaccgatg
      181 gcgtagtaac actggatacc gcaaatgtta ctgataactt tgcggaagtt aacgcgactg
      241 cagttggttt gttgccaaaa gaatttatga tttctgtaaa ttgtgaacct ggtaagacag
      301 aagcctcttt aactatgggt tccgcaagct acgcaaacac tagtggtact ttgaataaca
      361 atatgaacat cactgtaaat ggcattcagc cagcagagaa tgtgaatatt gcagttcata
      421 acatgaccaa caaatctggc gcagttgaga ttaaacaggt acacatgaac aactctgcag
      481 aaaaacatct gctgactttg gatgctaatg gcgacggtca atatgtgttt aatgcctcct
      541 atgtaaaagc accgaacagc caggatgtga cttctggaca cgttaccact aacgcactgt
      601 acaccattac ttataactaa tggtgcaata agcataaggt ttgcctgcgg gcaaacctta
      661 tttttccgtt ttcacataat tacacaatag catggagtgc ttatgtttcc cattataaaa
      721 aaaaccgtca gcgcgttatt tattagtacg ttgttaacca gtgcacctgc attcgccgat
      781 atcataattt ccggcactcg tatcatatat aatgcagata aaaaagatgt taacgtccgt
      841 ctggaaaaca aaggcaatcg gcccttgcta attcaaaact ggttggatac aggtgatgac
      901 aatgcggatc ccagccagat caaagttccg tttacttcta cgccgcctgt atctcgcatt
      961 gaatctaaac gtgggcaaac ggtaaaggtg atgtttactg gaacaacaca acttcctgcg
     1021 gaccgggaaa gtgttttctg gtttaacgtg ctggaagtcc cgcccaagcc gaaagatgcc
     1081 gaatcagaca aaaacttatt acaactggct ttccgtaccc gtatcaaact tttttatcgc
     1141 ccaagtggat taaaaggtga acccgctgaa gcaccagcca aactgacgtg gaagttaaat
     1201 aatgcgcagc ttcaggtaaa caacccaacc ccttactacg tttcattcaa tgacgtcgca
     1261 attgaaagcg gcggtaagag ctacaaagtc gactccagca tggttgcgcc ttttggtcag
     1321 gttagttttg ccgtgacaga tatagcgagc agtgtgtctt caggaaaagt tatttacaaa
     1381 gccattaatg attatggtgg caatattgat ggctccacat cgctttaatc actaaacgga
     1441 ttaactttaa atcgccagga acgcaacctt gtatacgtta actcatcaga aaagccgtct
     1501 accaaaaaca acacttctgg ctgcatgttg tgcttttttt tatagcagta acggcgcggc
     1561 aacagaaagc gtggaatatg acagttcctt tttgatgggt actggggcat cgagcatcga
     1621 tgtcaaacgt tattcacagg gtaacccaac gcctcctggc gtttataacg tccgtgtgtt
     1681 tatcaaccaa caatccgttg ccagtttaga acttcctttt gttgatatag gggaaaacag
     1741 cgccgcggcc tgtataacac ttaagaatct ggcacaactg catatcaaga agccagaata
     1801 cccaattaca ctgcttgcca gagaaggcga agaaggcgat tgcctggaca tcaaaaaatc
     1861 gatcgagcaa gcggaagtca gctatgacgg cagtgagcag catttggaaa tctcggttcc
     1921 gcaggcttac gtctataaaa catatggcgg ttatgttgac ccctcacttt gggagtcggg
     1981 tattaatgcc gccatgcttt cttactcatt aaacgcctac cactccgact ctaaaaatgg
     2041 caaccgggac agtatatacg gagccttcaa taccggtctg aatttaggag cctggcacct
     2101 gcgtgcgcgc ggcaactata actggtcaca agataacggt aacagttttg actttcagga
     2161 tcgttactta cagcgtgaca tcccggcaat acgctcacaa gtgatcgtgg gtgatgccta
     2221 taccaccggt gaaaccttcg actcagtcaa tgtacgtggt atgcgtcttt atagtgatag
     2281 ccgtatgcta ccatccgcac tggccagcta tgcgccaatc attcgtggcg tagcaaattc
     2341 taacgccaaa gtgacagtga cccagaacgg ttacaaaatc tatgagtcca ccgttccacc
     2401 gggtgaattc gtgattgacg atctcagtcc ttcagggttt ggtagcgaat tggtcattac
     2461 tattgaagaa gcagatggtt ctaaacgctc atttacccaa cctttctctt ctgtcgtgca
     2521 gttgcagcgc cctggcgttg gccgttggga tattagtgcc ggacaaatta tcgatgacag
     2581 cttgcgtcat gaacccaata tggcacaggc ctctttttac tacggtttca ataatttatt
     2641 caccggttat accggagtcc aaattacaga taacgactat atgtctggcc ttttggggct
     2701 agggatcaat accagtattg gtgcctttgc ggtagatatt acacatgccc gtaccgaaat
     2761 tcctgacgat aaaacatatc aggggcaaag ttatcgtata acctggaaca aattaatcga
     2821 agctacaagc acctcattta accttgctgc ctatcgttat tccactgagg attaccttgg
     2881 tctgcatgat gcgttagccc tcattgacga cgccaatcat ctgtcagtca acgacgacaa
     2941 agacacgatc cgtacctatt cacgcatgaa aaaccaattt accgttagtg taaatcagcc
     3001 gttgagtttt gcttatgagg attacggttc actcttttta tctggcagct ggacaaacta
     3061 ctgggctggt aacaataacc gaactgaata caatgttggt tacagtaaaa gtgtctcctg
     3121 ggggaatttc agcgtcaact tgcaacgtag ctggaatgag gacggcaata aagatgatgc
     3181 gatgtatttg aacgttagcg ttccgctgga aaatattttc ggcggtaaac gtaaatcctc
     3241 agggttccgt aacctaagca ctcaattcaa tactgatttc aacggttcgc atcaattaaa
     3301 cgtcagtagt tcgggtaaca gcgaagataa tctcatcggt tacagtgtga atacgggtta
     3361 taaccttgat aaagaatctg aaaatgtcgc ttccgttggt ggatatctta gctatgactc
     3421 cacatgggga ggtttttccg cttctgcttc tgctagcacc gataacagac aacaatactc
     3481 tgtctctacc gatggtggtt ttgtacttca cagtggcggc ctgaccttta ccaataacag
     3541 tttcggtagc aatgacactt tagtcgtcat taaagcaccc ggagcgaagg gggcacgagt
     3601 caataacggt actgatgaaa tcgatcgctg gggttatgcc gtttcctcat ccttaagtcc
     3661 ataccgtgaa aaccgggtgg gattaaatat tgaaacattg gaaaacgatg tcgaactgaa
     3721 aagtactagt gccaccactg tacctcgtag tggttccatt attcttgcca gttttgaaac
     3781 tgaccagggg cgttctgcag ttctgaatat cagcaccagt aacggtaaac ctattccttt
     3841 tgccgctgaa gtttatcagg atgaaattat gatcggcagt atgggtcagg gtggtcaggc
     3901 atttgtacgc ggtattaatg acagcggaga gttaattatc cgctggttcg aagacagtcg
     3961 aacaataaat tgcaaattgc attatcaact tcctgcacag ccagaaacac ttggaagtac
     4021 aaacacctta ttattaaaca accttacctg taagttggtt aaccactaaa atggaacatt
     4081 ctatgaaaag gatcataata atatctacgc tagttggcct tgggttaccc acaactggct
     4141 cagcgacaaa tattaatgtc gattttaccg cacaattgct ggcgactact tgtacaataa
     4201 cgattaaaga agatggaggc cccgcggtta ataatacggg cggtgatgat tatagtatga
     4261 ctatcccaac ggttgggctg gataaaattg ccaatgctca tgctgatgct caagccaatt
     4321 ttcaattggt ggccaaagat tgtagtgagg gttattcgaa aattatcact aaactttctg
     4381 gagcaaccat ttccggtaac ttgattaaaa acgaagcgac ggacactcct gtagcaacca
     4441 atattggtat gggcatcaag cgccgggatg cagctgatgc ttcgtttatt acgcctaata
     4501 atagtgcaag tgttgagtgg actacggaag ataaaactaa tggaatgaag ttaaccgttg
     4561 ccctgcgcga aactacagca gggacaggaa atatcggtaa tttccgtgcg aaagccacct
     4621 ttaacttcac ctacgaatga caggaacagc gaccatgaaa ttaaaagcaa tagcaatact
     4681 gattgttact ggtggaatga ttagccatag tgccctggca ggaacaacgt ctgcaacctt
     4741 aacggtagac agcattgtca caatgggtac ttgtactgct caattactgg actccaatga
     4801 taaagaaatc tcacaagttg cgattggtga cgtttatatt tctgaactaa gaaatgaaac
     4861 caataaaaaa gcctttaaaa tccgttttag tggttgtgcc ggacttccgc aaaaaaagca
     4921 ctggtaaaat taactcccag aggaataggt tgcggtggtt ataatgctaa ctcagccggt
     4981 tttgcaaatg caaagaatgg tgcgggcgca gcaggaagag ttgcagttga agtatggact
     5041 accaaaaatg cagaaattga agctggcagt gaaattttta attgttttag ccccgcagat
     5101 aaaacggtta acctttcttc cgtcactgcg acgcaacatt tcgattatga tttaagtgct
     5161 aagatggttg ttgctgaagg cagacagttg agtgatgtca caccgggtga gtttctttct
     5221 ccagctacct ttacaatctc ttatcagtaa caacagataa ggaaattata atgcttttcc
     5281 aggaacataa tttcgcactt tttttcgggc tggtaacagc cctctgctct cctgttagcc
     5341 tggctaataa agatgtagat cttactgcga acattgtgtc aagcacatgt cagatcgaaa
     5401 taaataataa tggtgtagtt gatctcggca ccgtgacact ggactatttt tctaacaata
     5461 taaccccaga tacggattac gctggcggta agaattttac tgtcaacgtc gtttcttgtg
     5521 ataatttgca aaccacacaa tcgcagatta aattcgattt tcaaccacaa actggatctc
     5581 ttgcccaagc aaatcaacaa gttttcagta accaatacga gttacaatct atcggagcta
     5641 aaaatgttgg ggttgttatt ttttcagcac agccccatgc acaaaaattt aacgttcggg
     5701 ggacggacgg agcatcaaaa gccatttatg ccttaccctc taatcaagtg gttccgtcaa
     5761 cgtggacatt ctatgcccga atgcaaaaag tcaataacgc tcttgcacct gttgctggca
     5821 ttgtgagaag tcaggttata gttaacgttt attacgaata ataaggatta ctatgcaagg
     5881 gaaagtcaaa aaaattttgc ttcatatata cagtgcagtg ctattatttt tggcatcgtc
     5941 atggtatgtt tttgctggca cgggttctga atgctatata gaaagcagtt cggccgggag
     6001 ttataccatt ttcattaata cccctcccta tagtgtagcg acacagcagg tcactactcc
     6061 ttacgaaata tctgatgcag ccacagatgt accaattgta cttcggggtg atggaattgc
     6121 atgtaaagcc ataccaaatg gcgatgagag tatacatttt cttaataccg ctgatgccaa
     6181 tctaatatca acatatactt cagcacgagg ttctacatta ttaaaaacca ccgttccggg
     6241 tattgcttat actgttgaat taatttgtga tacctgtcct gaagtagtcg acttacgcat
     6301 tccaccagct ggcgacgata acttcacccc caactccgat atctggtggg tgtggggcga
     6361 aacagatgaa aaatggaagt tacgttttcg ctttttctat actccggagt ttaaacctaa
     6421 aaatggtgtt accagtggaa cgttattacc gggaaaaatt gcttcctggt atattggtat
     6481 caacacccaa ccatggatca acttttacgt tgatgcagat acatttaaat ttaccgtgga
     6541 tcaacctaca tgtagtacca ttgctctgga ttcaaccagc caaaatatca gcggtaataa
     6601 aataaacttg ggagactatt atgtttccca ggttaagcag gaaatcaccc aagtagttcc
     6661 atttactatt cggggcgatt actgttacgc aagcaaaatt acagtgaagt tgaaagccac
     6721 aactgaggca ccaaataaaa aattaattgg aaaatcatcc ggctcagcaa ccggtgttgg
     6781 tgtaaaagtc tactcaactg cgaacaacag tgaggttcag ctaaacgcag acaacagcaa
     6841 cgagattata tttaactacg ctaactggtc aaacaacctt ctttattttc cgtttacggc
     6901 gcaactggtt aaagatggaa gtaatggcac aattagccct ggggatttta ccggtaatgc
     6961 gacattctct ttttcctatg aataagtaag tctcacccca actcaacgca ccagattcaa
     7021 actctggtgc gaaactttct tcacactaaa aaccattgcc cccctgattt cgactgttag
     7081 aatgcaatca gatctgactt tagctatcag gatacgttat gaaaccaacc accatctcct
     7141 tactgcagaa gtacaaacag gaaaaaaaac gtttcgcgac catcaccgct tatgactata
     7201 gcttcgccaa actctttgct gatgaagggc ttaacgtcat gctggtgggc gattcgctgg
     7261 gcatgacggt tcaggggcac gactccaccc tgccagttac cgttgccgat atcgcctacc
     7321 acactgccgc cgtacgtcgc ggcgcaccaa actgcctgct gctggctgac ctgccgttta
     7381 tggcgtatgc cacgccggaa caagccttcg aaaacgccgc aacggttatg cgtgccggtg
     7441 ctaacatggt caaaattgaa ggcggtgagt ggctggtaga aaccgtacaa atgctgaccg
     7501 aacgtgccgt tcctgtatgt ggtcacttag gtttaacacc acagtcagtg aatattttcg
     7561 gtggctacaa agtacagggg cgcggcgatg aagcgggtga tcaactgctc agcgatgcat
     7621 tagccttaga agccgctggg gcacagttgc tggtgctgga atgcgtgccg gttgaactgg
     7681 caaaacgtat taccgaagca ctggcgatcc cggttattgg cattggagca ggcaacgtca
     7741 ctgacgggca gatcctcgtg atgcacgacg cctttggtat taccggcggt cacattccta
     7801 aattcgctaa aaatttcctc gccgaaacgg gcgacatccg cgcggctgtg cggcagtata
     7861 tggctgaagt ggagtccggc gtttatccgg gcgaagaaca cagtttccat taaggagtca
     7921 cgttgtgtta attatcgaaa ccctgccgct gctgcgtcag caaattcgcc gcctgcgtat
     7981 ggaaggcaag cgcgtggcgc tggtgcctac catgggtaac ctgcacgatg gccatatgaa
     8041 gctggtcgac gaagccaaag cccgcgccga tgtggtcgtc gtcagtattt tcgttaaccc
     8101 gatgcagttc gaccgcccgg aagatctggc tcgttatcca cggaccttgc aggaggactg
     8161 cgagaagcta aacaaacgta aagtggattt agttttcgcc ccttcggtaa aagagatcta
     8221 cccgaacggt actgaaaccc acacttacgt tgacgttcct ggcctttcga ccatgctgga
     8281 aggtgccagc cgtccgggac attttcgcgg cgtttcgact attgtcagca agctgttcaa
     8341 cctggtccag ccggacatcg cctgcttcgg tgaaaaagat tttcagcaac tggcgctgat
     8401 ccgcaaaatg gttgccgata tgggcttcga tattgagatt gtcggtgtgc caattatgcg
     8461 cgccaaagac ggtctggcgc taagttcccg taacggttat ctgacggcgg aacaacgcaa
     8521 aattgcgcct ggtctgtaca aagttttaag ttcgattgct gacaaattgc aggctgggga
     8581 acgggatctc gatgaaatta ttaccattgc ggggcaagaa ctgaatgaaa aaggcttccg
     8641 cgccgatgat attcagattc gcgatgccga cacattgctg gaagtttctg aaaccagcaa
     8701 acgggcagta attctggtag ccgcctggct tggcgatgct cgcctgatcg acaacaaaat
     8761 ggtcgagctg gcgtaatact taactggcgc tacggctgat ggcgccagtt attaatttac
     8821 cccacgtcat ctgtcataac acaggtaact taaataaggt taattacctt atcaatctca
     8881 gcaagaggta aatttgccat ctctgcaacg tcttcccgag acattccttt actcagaaga
     8941 cgctgggcga attcctgact tacttcctgt cttccctgct gaatcccctt ctcaatccct
     9001 ttctcttcaa accactgcgc cagcgtcatc atagactccc ctcccgtttc cctgtctctc
     9061 aacacaccgt aaaacaaatc cgcttgttca gtatgaccgc gttgcagcat atagttttgc
     9121 atggcaacta actgacttcc gctagtgtac ccttcgtcga tcagcgtgac cagttgctca
     9181 agcaataaca ttaagtcgcg ctggcgaata tgtttttgca gtagttcgag aatcgcaatc
     9241 cgccgatgtt gcatgatttc gtcatccggt gtgatggtga tatccaccag cgggaaagga
     9301 ctgttataga cgcgtcgcgc cagctccggc gagtaaaaca tatcaaacca gcacattgat
     9361 agcggataag gtgtggcctc gccctgataa aacagtatcg gcaccaccag cggcagctta
     9421 tcgtggtcag cctccagatg ccggtgcatg gcggctatag aataacgcat catgcgaaag
     9481 gccattttct tatccggctt gctttggtgt tcaatcacaa catgcagata accgggattg
     9541 ccctgcattt gcacggaata gagcacgtcc gtactgtgtc ctttcaggct ctcttcaatg
     9601 aaactccccg actctaaatg aagcgtgttg aggtcacaaa gttcgcgtaa ttccactggc
     9661 aaatgtatct ccagaaagtc gcgagccgtc tccgcatgca ttaaaaattg tttaaatacc
     9721 gcgtcatgcg gtgtggtact cggtgcatcc atcttccctg tcttccgtgt ggctttgcgt
     9781 gaaggcagta tccagtcaag gcgcgctatc gtcagccacc tctttattgt ttaccgagca
     9841 gcgttcagag aaatatcaat tcgttacagg cgatacatgg cacgcttcgg cgcggtacgc
     9901 aaactgctga cgcaaatgcc gtcagatgag tagacactaa acaaaaatcg ggcaatactg
     9961 cgtgagaatt tcctcaaagc aggcatcgcc tgcttcgtta acgacagggt agaaaggtag
    10021 aagttatgat tcgcacgatg ctgcagggca aactccaccg cgtgaaagtg actcatgcgg
    10081 acctgcacta tgaaggttct tgcgccattg accaggattt tcttgacgca gccggtattc
    10141 tcgaaaacga agccattgat atctggaatg tcaccaacgg caagcgtttc tccacttatg
    10201 ccatcgcggc agaacgcggt tcgagaatta tttctgttaa cggtgcggcg gcccactgcg
    10261 ccagtgtcgg cgatattgtc atcatcgcca gcttcgttac catgccagat gaagaagctc
    10321 gcacctggcg acccaacgtc gcctattttg aaggcgacaa tgaaatgaaa cgtaccgcga
    10381 aagcgattcc ggtacaggtt gcttgattgt tatccctgcg gctggttact caccagccgc
    10441 gacatcgtct ccagcgaatc cgttcttaag atataaagtc gttttagtaa caacggatta
    10501 tcccccggtt ttactttgcc tttcatggtt gtcaccgcca ggtgaaatcc ggcatcgttt
    10561 gctgccttca cggcgttgtc attaaatccg ccaaacggat acgaaagata ccagacatgc
    10621 ggattaaatt gcgccagagc gcggcgtgaa cgtgcaaaat caaacagaat attgtgctca
    10681 ctacggctca gtaatatggg tcggcgataa ccatctaccc gatgcaaaaa atgggtatgt
    10741 gactggaaat caaatacatc gcgaatttcg ttaagctcag aaacgctcat aaattgcagc
    10801 gattttgggt tccacttctg cgggtgacgt ttgatgcgtg aggtaacaat aaacgccgtc
    10861 gccttcatgc catattgttt caacacagga tacgcatagc ggctcaccga cttgaggcca
    10921 tcatcaaagg taatcaccac cgctcgcgca gggagattga tcttattctt cacgtagcct
    10981 tccagctgca ccatgctcag tgtcgcgtat cccctgtcac gcagccaggc catctggtta
    11041 ttgaaagcgc gtaccgatgt ggtcgtcgaa gtatggcgaa aacgggtgtt ttcttcgtcg
    11101 cgcagaatat ggtgataggt tagcaccgac aggccattat cgggttgggc atccagtgcg
    11161 ctgatatagg ccagtcgatc gccaatacgg atctgatacc aggtttgatt taacctgtct
    11221 ttcagtttat gcaaaatcgg gtagcgcaaa ttgtccgcca gtaccccaaa tggcgcactt
    11281 cccgcactcg acgcgttata gaccggcgta tctttccagg taactaagtt ctgattactc
    11341 agcggcttgt tgaggtcgcc caaaccgtct tcaacttttt gtcgcccctg aaccggctcg
    11401 agatgacctt tatcgataaa acctttgcca aagccaaaat taaatgcgta ataacttgcg
    11461 gcagtgggct ccacggcaat gatttgtccg gcccgaatat tccccacggt caccatcttg
    11521 tcaccaattt gcgcccagac cgcagcattt tcgatggttt gcatataacg ggcaggtaac
    11581 gcggcactga cacttgcggt aaacagcatc agcaggagaa taacagcttg tttgtacatg
    11641 aaaaaataac cgagggtgaa acaacgcttt tattgtagca aaagcgccat cagaatcgcg
    11701 ggttaatact catacaaatt atgtttgagg acgaaaggcg gatttttttg ttgttggtgc
    11761 caaagactac tcacctccgg cgcacccagt tcgacaatac gctcgcgaaa ctctgaactg
    11821 gtcatggttt tacggcaagc catcatctgt tcaattaacg gtaacgtgac accagaaatc
    11881 acttcgcaac gggagtgttt gtggcttaat aatgaagcca cgcgatacgg tggcgctccg
    11941 gctatatcgg ttaagaagat gacaccctca ccagagtccg cttcatgcag agcatcgcac
    12001 atcatgcggc tgagcatatt agagcttaat ccgcgccaga aattcacggc ccggcactga
    12061 agaagtgccc catgtttttt ctccagcgca tccagcatct cttgcgcccg gtcatcgtga
    12121 caggtaatta cccaacctaa catcgccttc ctccttagcc gaaaatcagt ctaccctgac
    12181 cattgttgat aaagggtgat agccgtcaaa actcctctcc cccaaatcca gaggagagga
    12241 aatagactta gctacgcaaa ccacgtccac gttggatcag cgaccaacag atcaaataaa
    12301 acgccacaat aaagaccacc agtacgccaa aggtagtgac cagcggaaca tcattgatac
    12361 cgaggaagcc gtagcggaaa ccactgatca tataaacgat tgggttcagg tgcgacagcc
    12421 cttgccagaa cggcggcaac aaagtcagtg agtaaaagac cccgcccaaa tacgtgagtg
    12481 gcgttaacac aaaggttggc accaggctga tgtcatcgaa cgttttggca aacacaccgt
    12541 tcagcaaacc cgcaagggag aacaacaccg ccgtgagcac cagcgttaag gcaacgaata
    12601 cccacgaatg cacctgaaat ggcacaaaaa acagtgaaat tgccgtcacc agaatgccaa
    12661 caaacagacc acgcgccacg ccaccgccga catatccggc aataatgacg tgagtcggaa
    12721 ccggcgctac cagcagctct tcaatattac gctggaactt ggcaccaaaa aatgatgacg
    12781 caacgttggc gtaggcattg gtgatcaccg acatcatgat cagccccggt acgatgaact
    12841 gcatatagct gaagccatgc atatcgccaa tacgcgaacc aatcaggtta ccgaagataa
    12901 taaagtaaag ggtcatggtg atgactggcg gcaccagcgt ctgcacccag atacgcataa
    12961 agcgatggat ctctttcgcc cagatgcttt ttagcgccac ccagtaaaga tgcatcatgc
    13021 gcgatctcct tgtttttcat taaccagtga aacaaacagc tcttccagac ggttagcttt
    13081 gttacgcata cttaatacct gaatgccctg ctcacttaac tgcgtaaata cgctgttgat
    13141 cccctgctca cgcagcactt caacttccag cgtcgcggta tcgaccagtc gatactgata
    13201 gccatcgagc ttcggtaacg ggcttttcgg tgcgagatcg agaataaagg tttccgattt
    13261 cagcttcgcc agcagcgcct tcatcgaggt attttccacc agctcaccgt gttgaataat
    13321 gccgatattg cggcacagca tttctgcttc ttccaggtag tgtgtggtga gaatgatggt
    13381 ggtgcctttg tcgtttaaat ccttcaaaaa gccccacatt gagcggcgaa gttcaatatc
    13441 cacgcctgcg gtcggttcgt cgagaatcag tagtttaggt tcatgcatta acgcacgggc
    13501 aatcattaaa cggcgcttca tcccgccaga taacatacgc gcacgttcgt tgcgttttcc
    13561 ccatagatcg agttgtttaa gatatttttc gctgcggatg tacgcctctt tgcgctccac
    13621 accgtagtac cctgcctgat tcaccacaat ttgctgcacg gtttcaaacg ggttgaagtt
    13681 aaattcctgc ggcaccagtc ccaactgacg tttagcgttc acgacatctt tctcgagatc
    13741 gtaaccaaat acgctgaccc gcccggaggt tttatttacc agagagctga taataccgat
    13801 agtggtcgat ttcccggccc cgttcggccc gagaagcgca taaaaatcac ccgcttcgac
    13861 ctgcaaatct attccacgaa gcgcctgaac gccgcctgga taggtttttt taagctgttg
    13921 aagttccagt gcaatggtca taaattttta cttaccttac gttcttacac tttatctgtg
    13981 gtttaaatcg tcccggagtt gccctatatt agccaaacgt aattatttgg ttacaggtcg
    14041 ttaacctcca tgaaagacat agatacactc atcagcaaca atgcactatg gtcaaaaatg
    14101 ctggtggaag aggatcccgg gttttttgag aaactggcac aagcgcaaaa accgcgcttt
    14161 ctatggattg gatgttccga cagtcgcgtt cctgcagaac gtttaaccgg tcttgagccg
    14221 ggcgaactct ttgttcaccg taatgttgct aacctggtca ttcacaccga cctgaactgc
    14281 ctttccgtgg ttcagtatgc agtggatgta ctcgaagttg aacacattat tatctgtggc
    14341 cactacggtt gcggcggcgt acaagccgca gttgaaaacc cggaactggg gcttatcaac
    14401 aactggctgc tgcatatccg cgatatctgg ttcaaacata gctcattgct cggcgaaatg
    14461 ccgcaagagc gccgtctgga taccttgtgt gaactgaacg tcatggaaca ggtttataac
    14521 ctgggccact ccaccattat gcaatcagcg tggaaacgcg ggcagaaagt taccattcac
    14581 ggctgggcct acggcattca cgacggcttg ctgcgtgatc tggatgtcac agcgaccaac
    14641 cgcgaaaccc ttgagcaacg ttaccgtcac gggatttcca acctcaagct gaaacacgcc
    14701 aaccacaaat aaaaatgcca tgccggatgc aacacatccg gcaacttcac acttactcgt
    14761 ccagcagaat cactttgccg atatacggca gatgacggta acgctgtgcg taatcaatgc
    14821 cgtaacccac cacaaactca tccgggatcg agaaaccgat aaattcaacc gggacgttca
    14881 cttcacgacg ggacggttta tccagcagcg tacaaatcgc caacgacttc ggttcgcgca
    14941 ggcttaagat ctcacgcact ttcgacagtg tattccccga gtcgatgata tcttcaacaa
    15001 tcagcacgtc cttgccacgg atatcttcat ccagatcttt gaggattttc acatcacggg
    15061 tggtggacat gccgctaccg tagctggagg cggtcataaa gtcgacttca tgagatacct
    15121 gaacttcacg gcacaggtcc gccataaaca taaatgagcc acgcagcaga cccaccagca
    15181 ccatatcgct gccgctgtct ttgtaacgct cagtaatctg acgacccagt tcggcgatac
    15241 gcgctttaat ctccgcttcg gggatcatta cttctacagt atgtttcatc tctctaacca
    15301 tatgatttaa aaacaaatca cttcgcacgg gcaggttttg gcaccgcaac aaagcgcaag
    15361 acacgcagta taccagtaaa actattcatt cgcggaatct gttaataaag ctcgaattgt
    15421 gatgacgatc acacatgtta aaacctataa ttaattgcta ctaaaatatt aatgaattga
    15481 aatggtgtct ctttatggca attaacaata caggctcgcg acgattactc gtcacgctaa
    15541 cagccctttt tgcagcgctt tgcgggctgt atctactcat tggcggaggc tggctggtcg
    15601 cgattggcgg ctcctggtac taccctatcg ctggccttgt gatgctcggc gtcgcctgga
    15661 tgctgtggcg cagtaaacgt gccgcgcttt ggctatacgc cgccctgctg ctcggcacca
    15721 tgatttgggg cgtctgggaa gttggtttcg acttctgggc gctgactccg cgcagcgata
    15781 ttctggtctt cttcggcatc tggctgattc tgccgtttgt ctggcgtcgc ctggtcattc
    15841 ctgccagcgg cgcagttgcc gcactggtgg tggcactgct gattagcggt agtatcctga
    15901 cctgggccgg atttaacgat ccgcaggaaa tcaacggcac cttaagcgcc gatgccacac
    15961 ctgcggaagc tatctcaccc gttgccgatc aggactggcc tgcctatggt cgcaatcagg
    16021 aaggtcaacg cttttcacct ctgaaacaaa ttaacgccga taacgtccat aatctgaaag
    16081 aagcctgggt gttccgtact ggcgatgtga agcagccgaa cgatccgggc gaaatcacca
    16141 atgaagtgac gccgattaaa gtgggcgaca ccctttacct gtgtaccgct caccagcgcc
    16201 tgtttgcgct cgatgccgcc agcggcaaag agaaatggca ttacgatcct gagctgaaaa
    16261 ctaacgagtc tttccagcac gtaacctgcc gtggcgtctc ttatcatgaa gccaaagcag
    16321 aaacggcttc gccggaagtg atggcggatt gcccgcgtcg tatcattctt ccggtcaatg
    16381 atggtcgact gattgcgatt aacgctgaaa acggcaaact gtgcgaaacc ttcgccaata
    16441 aaggcgtgct caatctgcaa agcaatatgc cagacaccaa accgggtctg tatgaaccga
    16501 cttcgccacc gattatcacc gataaaacca tcgtgatggc aggttcagtt accgataact
    16561 tctcaacccg cgaaacgtct ggcgtgatcc gtggttttga tgtcaacacc ggggagctgc
    16621 tgtgggcttt tgatcccggc gcgaaagatc cgaacgcaat cccgtctgac gaacacacct
    16681 ttacctttaa ctcgccaaac tcgtgggcac cagcggccta tgacgcgaag ctggatctgg
    16741 tgtatctgcc gatgggcgtg accacgccgg atatctgggg cggtaaccgc acaccggaac
    16801 aggaacgtta tgccagctcg attctggcgc tgaatgccac taccgggaaa ctggcgtgga
    16861 gctaccagac cgttcaccac gacctgtggg atatggatct tccggcacag ccgacgctgg
    16921 cggacatcac cgttaatggt cagaaagtgc cagttattta cgctccggcg aaaaccggca
    16981 acatttttgt gctcgatcgt cgtaatggcg aactggtggt tccggcaccg gaaaaaccgg
    17041 ttccccaagg tgctgccaaa ggcgattacg ttacccctac tcaaccattc tcggaactga
    17101 gcttccgtcc gacgaaagat ttgagcggtg cggatatgtg gggagccacc atgtttgacc
    17161 aactggtgtg ccgcgtgatg ttccaccaga tgcgctatga aggcattttc accccgccat
    17221 ctgaacaggg tacgctggtc ttcccgggta acctggggat gttcgaatgg ggcgggattt
    17281 ccgttgatcc aaatcgtgaa gtggcgattg ccaacccaat ggcactgccg tttgtttcga
    17341 aactgatccc gcgtggtcct ggtaacccga tggagcagcc gaaagatgcc aaaggcacgg
    17401 gtacggaatc cggcattcag ccacagtacg gtgtaccgta tggtgtcacg ctcaacccgt
    17461 tcctctcacc gtttggtctg ccatgtaaac agccagcatg gggttatatc tcggcgctgg
    17521 atctgaaaac taatgaagtg gtgtggaaga aacgtattgg tacgccgcag gacagtatgc
    17581 cgttcccgat gccggttccg gtgccgttca atatgggtat gccgatgctg ggcgggccaa
    17641 tctccacggc gggtaacgtg ctgtttatcg ccgctacggc agataactac ctgcgcgctt
    17701 acaacatgag caacggtgaa aaactgtggc agggtcgttt accagcgggt ggtcaggcta
    17761 cgccaatgac ctatgaagtg aatggtaagc agtatgtggt gatctccgca ggcggtcacg
    17821 gttcatttgg tacgaagatg ggcgactata ttgtggctta tgcgctgccg gatgatgtga
    17881 agtaagactt gctcagattg ctgacaacgt gcgcgttgtt catgccggat gcggcgtgaa
    17941 cgccttatcc agcctacaaa attgtgcaaa atcaatggat tgcacagcta gcgtaggcct
    18001 gataagcgta gcgcatcagg cagatttgcg tttgtcatca gtttaatgcc cggagagatc
    18061 cgggcatatt tccgaatacg gtctttttat accgtaaacc ctaacatcat ccccgtatct
    18121 tcatgctcca gcagatggca gtgcgccata taagcatgtt ctttcggtgc atcgtgatta
    18181 aacttcacca gcacttcgct gacattacct tctaccttaa cggtatcttt ccagcccgcg
    18241 cgatgagccg ctggcggttt gccattttct gacaagatac ggaactgcgt gccgtggata
    18301 tggaacggat gcagcatcat gtcgcccacg ccagagataa cccaacgttc gtattgccct
    18361 ttcgccgccg caaacatcgg cttgttcata tcaaacgcct gaccgttgat tttgttggca
    18421 tggtggaaat cgaacttccc gccgtggttc atatgattca tattgccgtg ccccatatgg
    18481 cccatcatct ggctgtgatc catcccggcc atcgcctgat cgccatattt ctccattagc
    18541 atctgcatcc ccatcatatc gagcatcggg tccatagaga gttgcagctt gcgtaccgtc
    18601 agcccttcca gcgaaggtaa cgcaggcagg ctacttaatg tgtctggcaa agcaccggag
    18661 gcactaatag caatcggctg aatccgcatt accggatgag gcttatcaaa cggcgcaatc
    18721 gccatcccca tctggctgac cggcagcgtc accaggtcaa agggtttgtt atcgttaacc
    18781 tccaccagca cttcaaaacg ctcgcccatc agcaccggca gttcgctcac cttcactggt
    18841 tcaggtagca gaccaccgtc gctggcaatc acatacagcg ggcgattgtc gctggtggcg
    18901 aaattgagcg aacgggcatt acagccattg agcaaacgca ggcgcagcca accacgcggg
    18961 gcagcgtgtt gcgggtagat tgcaccgttg gtcagcaacg tatcgccaaa ccagcccacg
    19021 gcggcggtca tcacatccag ttgataatca atctgcccgt cggcgctaaa tttcttatcc
    19081 tgaacgatca ccggaacatc atcgataccc cactgttttg gcagcattaa tttcaggatc
    19141 tcgtcatctt caatcaccac cagcccagcc agccccatcg ccacctgtcg cccggttttg
    19201 ccgtgctgat gcggatggaa ccagcaggta gcggcaggtt gatcaacgtt caacgtcacc
    19261 gagcgcttgc cacctggcgg aataattccc tgcgggccgc cgtcgacttc acccggtact
    19321 tccagcccgt gccagtgcaa cgttgtctct tccgtcagtt ggttgtagat atcaaccgtt
    19381 accgctttgc cgcgctgtaa tttcaccgcc ggccccagca gattgccgtt atagccccag
    19441 gtagttgcag ttttcccgcc aaaggtggac tggcctgcgc caatagttaa ctgaatgcga
    19501 ttacgggcat cggtcgtgag caaatcaggg atcggtaacg ttgggcgttc tgccgcaaat
    19561 actgcgcggc tccacagcgg caaagccgaa gccacaccca gcgcgacgga atattttaag
    19621 aaatcacgac gttgcatagt tatttcctta ttcttaagca ggcgaaacaa aatcaaacgt
    19681 tgagcatagt ccttcccctt acgggaaggt caagccgcag acacaattta atcgacaata
    19741 atggtcgtcg cctcgctcgc agtgtgctaa cgtttatctt ctttaagccc ctggtagaag
    19801 caatgaagac gtttttcaga acagtgttat tcggcagcct gatggccgtc tgcgcaaaca
    19861 gttacgcgct cagcgagtct gaagccgaag atatggccga tttaacggca gtttttgtct
    19921 ttctgaagaa cgattgtggt taccagaact tacctaacgg gcaaattcgt cgcgcactgg
    19981 tctttttcgc tcagcaaaac cagtgggacc tcagtaatta cgacaccttc gacatgaaag
    20041 ccctcggtga agacagctac cgcgatctca gcggcattgg cattcccgtc gctaaaaaat
    20101 gcaaagccct ggcccgcgat tccttaagcc tgcttgccta cgtcaaataa tccctgatac
    20161 ctttttgtag aaatattgac cgtgcatccg cggtcaatgt tagctattat gttgcgccct
    20221 ttttttacgg gtgttaacaa aggaggtatc aacccatggc cgaaaaaaaa cagtggcatg
    20281 aaacgctaca cgaccagttt gggcagtact ttgcggtaga taacgttctg tatcatgaaa
    20341 agaccgatca ccaggatctg atcatttttg agaacgctgc atttggtcgc gtaatggcgc
    20401 tggatggcgt agtacaaacc accgagcgcg acgagtttat ctatcatgag atgatgaccc
    20461 atgttccgct actggcccat ggtcacgcga aacatgtgct gattatcggc ggcggcgacg
    20521 gtgccatgct gcgtgaagta acccgacata aaaacgttga gtcaatcacg atggtggaaa
    20581 tcgatgcggg tgtcgtatcg ttctgccgtc agtatctacc caaccataac gccggtagct
    20641 acgacgatcc gcgctttaag ctggtgatcg acgatggcgt caatttcgtt aatcaaacca
    20701 gccagacctt tgatgtcatt atctccgact gcaccgatcc tatcggtccc ggcgaaagcc
    20761 ttttcacttc ggcattttat gaaggctgca aacgttgtct gaatcctggc ggtatcttcg
    20821 tcgcacaaaa cggcgtctgc tttttacagc aggaagaagc catcgacagc catcgcaaac
    20881 tcagccatta cttcagcgac gttggctttt atcaggcggc gatcccgacc tattacggcg
    20941 gtatcatgac ttttgcatgg gcgacagata acgacgcctt acgccatctc tcaaccgaaa
    21001 ttattcaggc gcgttttctc gcttctggcc tgaaatgccg ttattacaat tcggcaatcc
    21061 atacggcagc ttttgcctta cctcagtatc tgcaagacgc actggcttca cagccgtcct
    21121 aaggagaaga taagaaattg aaaaaactga aactgcatgg ctttaataat ctgaccaaaa
    21181 gtctgagttt ttgtatttac gatatctgct acgccaaaac tgccgaagag cgcgacggtt
    21241 atattgctta tatcgatgaa ctctataatg ccaaccgtct gaccgaaatc ctgtcagaaa
    21301 cctgttccat tatcggggcc aatattctta acatcgcccg ccaggattac gaaccacagg
    21361 gtgccagcgt cactattctg gtgagtgaag aaccggttga cccgaaactc atcgacaaaa
    21421 cagaacaccc cggcccactg ccagaaacgg tggttgccca tctcgataaa agtcatattt
    21481 gcgtacatac ctacccggaa agtcatcctg aaggcggttt atgtaccttc cgcgccgata
    21541 ttgaagtctc tacctgcggc gtgatttctc cgctgaaggc gctgaattac ctgatccacc
    21601 agcttgagtc cgatatcgta accattgatt atcgcgtgcg cggttttact cgcgacatta
    21661 acggtatgaa gcactttatc gaccatgaga ttaattcgat tcagaacttt atgtctgacg
    21721 atatgaaggc gctgtatgac atggtggatg tgaacgtcta tcaggaaaat atcttccata
    21781 ccaagatgtt gcttaaagag ttcgacctta agcactacat gttccacaca aaaccggaag
    21841 acttaaccga cagcgagcgc caggaaatta ccgctgcgct gtggaaagaa atgcgcgaga
    21901 tttattacgg gcgcaatatg ccagctgttt aacgtctcgg gcggagctcc caggctccgc
    21961 cagatttatt tacttctgct gcacgaaatt acggtaagcc gccacgacct gcagaaaatc
    22021 ctcaacgccg catagcgaca ggctttcttc gtcgtagtag ttcatcccct cttccatttc
    22081 atcgccagcg aattccagtt gattggcgcg aaccatcacc tcttcaccgt ccatccacag
    22141 ggtgtattca tgccctgccc gttgccagga ccgttcgcta cctttcagtg cgtgcgctgc
    22201 ttgttccact tcatcaagca aggccaggtt ttctttcacc tcttcattaa accagtgccc
    22261 gaccacttca tgccccatgg acatacgcac ctttaccact ccggtaatat cgcgcagaaa
    22321 ttcgtaatcc atagtgtgtt cctctgctcc cggcaatgcg ccgaaatcgc attgcgccat
    22381 tgatgtaatt atcgcagcct taccggagaa aaacagcgga acggcgatgg tttagaaata
    22441 aaaaatgccc gggaatctca ttcaccgggc attgtgtcgt ttatgcgcag cgcgtgcgct
    22501 tacttattac accgcagtct ggaaaatcac cccatcggct ttttcggtgt actgagaaag
    22561 ctggttgaag ttcagataac gataagtatc aacggcagtt ttatctactt gcgcaacgta
    22621 ggtctggtac tcttccggcg tcggcagttt gccaatcagc gccgcaacag ccgccagttc
    22681 tgcagaagcc aggaagacat tcgcgccagt ccccagacgg ttcgggaagt tacgggtaga
    22741 ggtggaaacc accgtcgcac cgtctgctac acgcgcctgg ttacccatac acagagaaca
    22801 gcccgggatc tcgatacgag ccccgctctt accgaagacg ctgtagtagc cttcttcagt
    22861 caactgtgcg gcgtccatac gggttggcgg tgccacccac aggcgggtcg gcaactggcc
    22921 tttatgcgca tccagcagtt tacccgcagc acggaagtga ccgatgttag tcatgcaaga
    22981 accgataaat acttcgtcga tcttctcgcc ctgtaccgca gacagcggac gcgcgtcatc
    23041 cgggtcgttc ggagcacaca ggattggctc tttaatatcc gccagatcga tgtcaatcac
    23101 tgccgcgtat tccgcatctg catcggcttc cagcagctca ggattcgcca gccatttttc
    23161 catgccctga atacgacgtt ccagggtacg acgatcgccg taaccttccg cgatcatcca
    23221 cttcagcagg acgatgttag agttaaggta ttcgatgatc ggttctttgt tcagcttgat
    23281 agtacaacca gcggcagaac gctcagcgga cgcatcggta agctcaaagg cctgctcaac
    23341 tttcagatcc ggcagacctt caatttcgag gatgcggcca gagaagatgt ttttcttgcc
    23401 tttcttctca acggtcagca gaccttgttt gatcgcatac agcggaatgg cgtgtaccag
    23461 atcgcgcagg gtgatgcccg gctgcatttt gcctttgaag cgcaccagaa cggattccgg
    23521 catatccagc ggcattacgc cagttgcggc agcaaacgcc accagaccag aacccgccgg
    23581 gaaagagata ccgatcggga aacgggtatg ggagtcacca ccggtaccga cggtatccgg
    23641 cagcagcata cggttcagcc aggagtggat tacgccgtcc cccggacgca gcgacacacc
    23701 gccacggttc ataatgaagt ccggcagcgt gtggtgcgtg ttcacgtcaa ctggcttcgg
    23761 atacgccgcg gtgtggcaga aagactgcat caccaggtca gccgagaagc ccaggcacgc
    23821 caggtctttc agttcatcac gggtcatcgg gcctgtggtg tcctgagagc ctacagaagt
    23881 cattttcggt tcacagtacg cgcccggacg aatgcctttc acgccacagg cacggcctac
    23941 cattttttgc gccagcgaga agccgcgatc gctctcagcg acatctttcg cctgacggaa
    24001 cacatcactg tgcggcagac caagtgcttc acgcgctttg gtggtcaggc cacgcccgat
    24061 aatcagcgga atacggccgc cagcacgcac ttcatcaatc agcacgtcgg ttttcagttc
    24121 gaaggtcgcc agcagttcgc ctgtttcgtg gttacgcact tcacctttgt acgggtaaac
    24181 gtcaatcacg tcgcccatgt tcaggttaga gacgtcgact tcgattggca gtgcacccgc
    24241 gtcttccatc gtgttaaaga agatgggtgc aattttaccg ccgaggcaca aaccaccgcc
    24301 gcgtttgttc ggcacatgtg gaatatcatc gcccataaac cacagaacgg agttcgtggc
    24361 ggatttacgc gatgaacccg tacccacaac gtcaccgacg tacgccagcg ggaaaccttt
    24421 ctgttgcaga gcttcaatct gtttgatcgg gccaacaaca cctggctggt ctggctcaat
    24481 accttcacgg gcgtttttca gcatcgccag cgcgtgcagt gggatatccg ggcgcgacca
    24541 cgcatccggt gccggagaaa ggtcatcggt gttggtttcg ccagtgactt tgaagacggt
    24601 aacggtcagt ttttcagcca gcgccgggcg attcaggaac cattcggcat ccgcccagga
    24661 ctgcattacc tgcttcgcat attcgttgcc tgctttggct ttctcttcta cgtcatagaa
    24721 gttatcgaac atcagcagtg tgtgagaaag tgctttggca gcgatcggtg ccagtttggc
    24781 atcatccagc gcgtcgatca gcggatgaat gttgtaacca ccctgcatgg tacccagcag
    24841 ttcgatggct ttttccggag tcagcagagg ggatttggct tcgcctttag cgatagcagc
    24901 caggaagcct gctttgacat aggcggcttc atcgacgcca gggggaacac ggttggttaa
    24961 tagatctaac aggaattctt cttcgcccgc gggcgggttt ttcagcagct ctacaagtgc
    25021 ggccatttgg tttgcatcca ggggtttggg cgcaatcccc tcagcggcac gctcagctac
    25081 gtgcttacgg tattcttcta gcacgacggt tctcctcgct ctcattgtca tagtgcggca
    25141 ggcgattctc ttcacgctcc tgtgagacag cagtttgtag ggtaaatgcc cagtaccgca
    25201 acgggcagaa tagcaggatt ttgacgacgt gttaatctgt ttacaaaaaa gcaacataaa
    25261 aaagtggctg aatcgttaag gatggtctag agattgtttc cctccgtaaa aacttcgtta
    25321 attcgcatgg tgataatcac ctttctcaac gaatcccaag attagtcaaa atttaaacta
    25381 ccgcctcttt atactcggat tcacagcacc tgcgggtggc agttcgccga ccattgcgat
    25441 ttccttgaga tccgaattat gaaaatgact ttgccgttta aaccccatgt gctggcgcta
    25501 atttgcagtg ccgggctttg tgccgcctct accgggctat atataaaaag ccgcacagtg
    25561 gaagcgcctg tggaaccgca atcgacacaa ctggctgtgt ctgacgctgc cgcggttacg
    25621 cttcctgcaa cggtttccgc acctcccgta acacccgccg tcgttaaatc cgcattcagc
    25681 actgcacaaa tagatcaatg ggtcgcgccc gtcgcgctat accccgactc tctgctttca
    25741 caagtgttaa tggcatcaac ctatccggca aacgttgctc aagcagtgca atggtcgcac
    25801 gataatccac ttaaacaagg cgatgctgct attcaggcgg tatctgacca gccgtgggac
    25861 gctagcgtca aatcactggt ggcctttcca caattgatgg cgttgatggg cgaaaacccg
    25921 caatgggtgc aaaacctggg cgatgctttt ctggcgcagc cgcaggacgt gatggactcg
    25981 gtacagcgat tgcggcaact ggcgcaacaa accggctcgc tgaagtcatc aaccgaacag
    26041 aaagttatta ccacaacgaa gaaaactgta ccggtaaaac agacagtcac ggctcccgtc
    26101 ataccatcca ataccgtttc aactgccacc cctgtcatta cagagccggc aacaaccgtc
    26161 atttccattg agcccggcaa tcctgatgtg gtctatattc ccaactacaa cccaaccgtg
    26221 gtttacggga actgggccaa tactgcgtat ccgccggttt atctgccacc accagccgga
    26281 gaaccgtttg ttgacagctt tgtgcgcgga ttcggctata gcatgggcgt tgctaccacg
    26341 tacgcactat tcagcagcat cgactgggat gacgacgatc atgaccatca tcatcatgac
    26401 gatgatgatt atcatcacca cgatggcggt catcgtgacg gtaatggctg gcaacacaac
    26461 ggcgacaaca tcaatatcga cgtcaacaat ttcaaccgta tcaccggtga gcatcttact
    26521 gataagaata tggcatggcg gcacaatcca aactaccgta atggtgtgcc ctatcatgat
    26581 caggatatgg caaagcggtt tcatcaaacc gatgtcaacg gcggaatgag tgccacgcag
    26641 ctacctgctc caacacgcga cagccagcgt caggcggcag caagtcagtt tcagcaacga
    26701 acacacgccg cccccgtcat tacacgagat acccaacgtc aggcagcggc acagcggttt
    26761 aatgaagctg aacactatgg gagctatgac gacttccgcg acttcagccg tcgccaacca
    26821 ctgatccagc aacaaaagga cgccgctcgt cagcgttatc agtcagcttc tcctgagcag
    26881 cgccaggcag ttcgcgagag aatgcagact aacccgcaga tccagcagcg aagagaggca
    26941 gcgcgtgagc gcattcagtc cgcctcgcct gagcagcgcc aggcagtccg cgagaaaatg
    27001 cagactaacc cacagatcca gcagcgaaga gacgcagcgc gtgagcgtat tcagtcagcc
    27061 tcgcctgagc agcgccaggt gtttaaggaa aaagtacagc agcgcccact gaaccaacag
    27121 caacgtgata acgcccgcca gcgtgttcaa tcagcatcac ctgaacaacg tcaggttttt
    27181 cgggagaaag ttcaggagag ccgcccacaa cgtctaaacg acagtaacca tactgccagg
    27241 ctgaataacg agcaacggtc agcagtacgc gaacgtctct ctgagcgcgg agcaaggcga
    27301 ctggaaaggt aaattacagg cgtaaaaaaa gcggcgtggt tagccgcttt tttaattgcc
    27361 agatgtcccg gcaaacgaaa aattacttct tcttcgcttt cgggttcggc aggtcagtaa
    27421 tgctaccttc gaacacttct gccgccaggc ccacagactc gtgcagagtc gggtgcgcgt
    27481 ggatggtcag tgcgatatct tcagcatcac aacccatttc gattgccagg ccgatttcac
    27541 ccagcagctc gccgccgtta gtaccgacaa tcgcaccacc gatcacacgg tgagattctt
    27601 tgtcgaaaat cagcttggtc ataccgtctg cgcagtcgga agcgatcgca cgaccagaag
    27661 cagcccacgg gaaggtggcg gtttcgtagc tgatgccttt ctctttcgct tctttctcag
    27721 tcagacctac ccatgcaact tctggttcgg tataggcgat ggacgggata actttcggat
    27781 cgaagtagtg tttcttaccg gcgataactt cagcggcaac gtgaccttcg tgaacacctt
    27841 tgtgtgccag catcggctga ccgacgatat cgccgatagc aaagatgtgc ggtacgttgg
    27901 tacgcagctg tttgtcaacg cggatgaaac cacggtcgtc aacttccacg ccagctttgc
    27961 ctgcgtcgag gtttttaccg ttcggcacac gaccaatcgc taccagcacg gcgtcgtaac
    28021 gctgcggttc agcgggtgct tttttgcctt ccatcgtcac ataaataccg tcttctttcg
    28081 cttcaacggc ggtaactttg gtttccagca tcaggttgaa tttcttgctg atacgcttgg
    28141 taaagacttt aacgatgtct ttgtcagctg ccgggataac ctggtcgaac atttcaacca
    28201 cgtcaatctg tgaacccagc gcgtggtata cggtgcccat ttccagaccg atgataccgc
    28261 cacccattac cagcaggcgt tctggtactt ctttcagttc cagcgcgtca gtggagtccc
    28321 agatacgcgg atcttcatgc ggaataaacg gcagttggat cgggcgagaa cccgctgcaa
    28381 tgatcgcgtt gtcgaagttg atcacggttt taccgttctc accttcaact tccagggtgt
    28441 tagccccggt gaatttaccc agaccgttga ccactttgac tttgcggcct ttcgccatac
    28501 cagccagacc accggtcagc tgattgatca ctttctcttt ccaggtacga atcttgtcga
    28561 tatcggtttt cggttcgccg aagacgatac catgttcagc cagcgctttg gcttcttcga
    28621 taacttttgc tacgtgcagc agtgctttag aagggataca gccgacgttc aggcaaacac
    28681 cgccaagggt gttgtaacgt tctacgatta cggtttccag acctaaatca gcgcaacgga
    28741 aggcagcgga gtaacctgcg gggcctgccc caagtaccac gacctgagtt ttgatttcag
    28801 tactcatcat gacctctata tatttatctc cggcggtcat acccgtcgtc tttcaggccg
    28861 cagcggcgtt agcttcacgc cctcacccca gtcacttact tatgtaagct cctggggatt
    28921 ctggcgcttg ctaccttgct gcacccagaa atccataggg tatgtgaagt aataccctaa
    28981 ccaccaccgg gtcgttctat ccgtcggtat tttacaaaat tgttaacaat ttttaaacaa
    29041 caaacggcaa ccgatttgtc tattagctaa taacctcaat acattcatga gattaccaga
    29101 aaaaagccgg ccgttgggcc ggctctttta cttacatcac cagacggcga atgtcagaca
    29161 gcgtgttgtt aatgatggta atgaaacggg caccatcagc accgtcgatc acgcggtggt
    29221 cgaaggagag agaaatcggc agcatcagac gcggcacgaa ctctttacca ttccacaccg
    29281 gctccatcgc ggacttggaa acgccgagga tagccacttc cggcgcgttc acaatcggcg
    29341 cgaagtgggt agtacccagg ccgccgatgc tggagatggt gaagcaaccg ccctgcattt
    29401 cgcccgcagt cagcttaccg tcacgcgctt tcttagaaat agtcatcagc tcgcgagaca
    29461 gctcgatgat gcctttcttg ttgacgtctt tgaataccgg aacaaccaga ccgttcgggg
    29521 tatccaccgc cacaccgatg ttgatgtatt tcttcagggt cagacgctga ccgtcttccg
    29581 acagcgaact gttgaagcga ggcatctgct caagagctgc agcaacggct ttcatgatga
    29641 agacaactgg ggtgatcttc acatccagct tacgtttcgc cgcttcttcg ttctgctgtt
    29701 tacggaacgc ttccaactcg gtgatatcgg ttttgtcgaa gtgagtaaca tgcgggatca
    29761 ttacccagtt acggctcagg ttcgcaccag agattttctg gatgcggccc agttccactt
    29821 cttcgatttc accaaacttg ctgaagtcca ccttcggcca cggcagcatg ccagggatac
    29881 caccgccagt cgctgccgga gctgcttctg cacgtttgat agcttctttc acgtaagcct
    29941 gaacgtcttc gcgcaggata cgacctttac ggccagtgcc cttcactttc gcaaggttaa
    30001 cgccaaactc gcgtgccaga cggcggatca gcggagtcgc gtgaacgtaa gcgtcatttt
    30061 cagcaaattc agatttgcct tcagctttcg cagccggtgc tgctgccggg gcttcagctt
    30121 ttgctgccgg agccggcgct gctgcttcct gtttcgcagg agctgccgca ggcgctgctg
    30181 cttcctgttt cgcaggagct gccgcaggcg ctgcgccttc aacttcaaag atcataatca
    30241 gcgagccagt tttcacttta tcgccaacgt tgactttcag ttccttcacg acgcctgcaa
    30301 acggagccgg aacttccata gaagctttat cgccttctac ggtgatcagt gactgttcag
    30361 cggcaacttt gtcgcccact ttcaccatca cttcggtcac ttcaacttcg tcaccgccga
    30421 tatccggaac gttaacttct ttcacgccag ccgctggtgc aggggccgct gccggagctg
    30481 cttcctgttt agcggccgga gctgccgcgc ctgcttcacc cgcgacttcg aagaccataa
    30541 tcagcgagcc ggtagacact ttgtcaccca cgttcacttt gatctctttc acggtgccag
    30601 caaacggagc cggaacttcc atagaagcct tgtcgccttc tacggtgatc agcgactgtt
    30661 cagcttcaac tttatcgcca actttcacca ggatttcggt cacttcaact tcgtcgctgc
    30721 cgatatccgg aacgttaacg tcttttgccg ccgcagccgc tggtgctgct gccggagctg
    30781 cttctttctt ctcttctgcc tgagcaggtg cagcgtctgc tgcaccgtcg gcggaatcga
    30841 aaatcataat cagtgcgccg gtctgggttt tatcgccaac agagactttg atctctttaa
    30901 cgatacccgc ctgcggagac ggaacttcca tagaggcttt gtcgccttct acggtgatca
    30961 gcgactgttc ggcttcaact ttgtcgccca ctttgaccag gatctcggtg atttcaactt
    31021 catcagcccc gatgtccggt actttgattt cgatagccat tattctttta cctcttacgc
    31081 cagacgcggg ttaactttat ctgcatcgat gttgaatttg gcgattgcgt cagcaaccac
    31141 tttcttatcg atttcgccac gtttagccag ttcgcccagc gccgcaacca cgacataaga
    31201 agcatcaact tcgaagtggt gacgcaggtt ctcacggctg tcggaacgac cgaagccatc
    31261 agtacccagt acgcggtagt cgtcagccgg tacgtaagta cggacctgct cagcgaacag
    31321 tttcatatag tcggtagatg ccactgccgg agcgtcgttc atcacctgag cgatatacgg
    31381 aacgcgcgga gtttccagcg ggtgcagcat gttccagcgt tcacaatcct gaccatcacg
    31441 cgccagctcg gtgaaggagg tcacgctata aacgtcagaa cctacgccgt aatctttcgc
    31501 caggatctca gctgcttcac ggacgtgacg caggatagaa ccggagccga gcagctgaac
    31561 tttacctttg ctaccttcaa tagtttcgag tttgtagata cctttacgga taccttcctc
    31621 agcaccttcc ggcattgccg gcatgtggta gttttcgttc agcgtagtga tgtagtagta
    31681 aacgttctct tgtttttcac cgtacatacg ctccagaccg tcatgcatga tgacagcaac
    31741 ttcgtaagcg taagccgggt cgtaagagat acagttcggg atagtcagcg actgaatgtg
    31801 gctgtgacca tcttcgtgct gcagaccttc gccgttcagg gtggtacgac cggaagtacc
    31861 gccgatcagg aagccacgcg cttgctggtc gccagccgcc cagcacagat cgccaatacg
    31921 ctggaagccg aacatcgagt aatagatgta gaacgggatc atcggcagat tgttggtgct
    31981 gtaagaggtc gccgctgcca gccaggaaca acctgcgccc agctcgttga tcccttcctg
    32041 cagaatctga cctttctcgt cttctttata gtaagcaacc tgctcgcggt cctgcggggt
    32101 gtactgctga ccgttcgggc tgtaaatacc aatctgacgg aacagacctt ccataccgaa
    32161 agtacgcgct tcgtcggcga tgatcggtac cagacgatct ttgatcgact tgttcttcag
    32221 catcacgttc agagcacgaa cgaaagcgat agtggtagag atctctttgc tctgctcttc
    32281 caacagcgcg ccgaagtctt gcaggctcgg cagctcaagc ttctcggtga agttcggctg
    32341 acggcttggc agataaccgt gcagtttctg acgctgagcg tgcagatagg tatgctcttc
    32401 agaaccttcc gggaaggtga tgtacggcag tttttcgata tctgcatcag acaccggcac
    32461 attgaaacgg tcgcggatgt gacgcacgcc gtccatgttc attttcttaa cctggtgcgc
    32521 gatgttttta ccttcagccg cgtcgcccat gccgtaacct ttaatggtat gagcaaggat
    32581 tactgtcgct ttgcctttgg tttcctgcgc tttcttgaat gcagcgtaga ttttcttcgg
    32641 atcgtgacca ccacggttca gtgcccagat ctgctcgtca gtccagtctg caaccagtgc
    32701 tgcggtttca ggatatttac cgaagaagtg ttcacgaacg tacgcaccat ctttcgattt
    32761 gaaggtctgg tagtcgccgt caacggtttc gttcatcagc tggatcagtt taccgctggt
    32821 atctttacgc agcagttcat cccaacggct accccacatc actttgatca cgttccagcc
    32881 agcaccttcg aagatgcctt ccagttcgtt gatgatcttg ccgttaccgg tgaccgggcc
    32941 gtcaagacgc tgcaggttac agttgataac gaagaccagg ttatccagtt tttcacgggt
    33001 agcgatggtg atcgcacctt tggattccgg ttcgtccatt tcaccgtcgc cgaggaacgc
    33061 gtaaacggtc tgtttagagg tatctttcag gccacggtgt tccagatatt tcaggaattt
    33121 agcctggtaa atagcaccaa tcggacccag acccatagat acggtcggga actgccagaa
    33181 ttccggcatc agtttcgggt gcggatagga agagaggcca ttgccgtgaa cttcctgacg
    33241 gaagttatcc agctgctcct gagtcagacg accttccagg aaagcacgag cgtacacgcc
    33301 cggggagatg tggccctgga agtaaaccag gtcgccgcca tcctgctcgt tgcgtgcacg
    33361 gaagaagtgg ttaaagcaca catcataaat ggttgcggaa gactggaagg acgccatgtg
    33421 gccgcccagt tcgagatctt ttttcgacgc acgcagcacc gtcatgatgg cgttccagcg
    33481 gatagctgaa cgaatacggc gttccagttc cagattaccc ggatactccg gttgttcttc
    33541 aacggggatg gtgttgatgt agttgctgat accggtgcct gcggctacgt tgacaccgcc
    33601 tttgcgggct tcagcaagca gttgttcgat cagatactga gcacgctcaa caccttcttc
    33661 acggatgacc gattcgatcg cctggagcca gtcgcgagtt tcgatcggat ccacgtcatt
    33721 tgggaaacgt tctgacatgg gttattcctt atctatctaa taacgttgag ttttctggaa
    33781 cctgtcccat tgaactctcg ccggaaagct caataagaca ggttctacgt ttagttgccg
    33841 cgcttttata tgcgcttgat ttacaacatc ttctggataa tttttaccag aaaaatcact
    33901 aattctttcg ttgctccaga cgacgcagag aacgctcacg gcggctctct tcacgacttc
    33961 tgtcgagcaa aatttcttcg ataaaagcca gatggcgatg cgatgcttcg cgcgcttctt
    34021 ccggcttacc ggccataatc gcttcaaata tgcgggtgcg gtgactactc accagcggca
    34081 gcatctcgcg acgcgaatag agcaattcga agttctggcg gacattctgg gccaacatcg
    34141 gctccataca ccttagcaga tgaagcagaa ccacattgtg ggccgcttca gtgacagcaa
    34201 tctgatactg gagtacggcg tttgattccg cgtccagatc gccagactgc tgcgccagtt
    34261 ctatggcgtg atggagttca cggatgcgtt ccttgtcttc atcggtacta cgcagcgcgg
    34321 cgtaataagc ggcgatacct tccagggcgt gtcgtgtttc gagcaagtca tactgtgact
    34381 caggatggtc ggagagcagc tccaccagcg gatcgctgaa gctttgccat aggctgctct
    34441 ggacaaaagt gccgccaccc tggcgacgaa gcaacaagcc cttcgcttcg agacgttgaa
    34501 tcgcctcacg caaggaggga cgggagacgt caaactgttt tgccagttcg cgttccggtg
    34561 ggagtttttc gcccgggcgg agagtgcctt cgaggatcaa aaactccagt tgctgctcaa
    34621 tcacatcgga gagttttggt tggcggattt tgctgtaggc catgagttcc tgtcttaagc
    34681 cacttgccga agtcaattgg tcttaccaat ttcatgtctg tgacgctaaa gtaacaaagt
    34741 attcacctta tgtccataca ggttttgatt gaaatcatga aactgtgcac attttaacaa
    34801 cttgacatat ataacgtttc aaagttgtaa ctatg
//
LOCUS       DAPHBJ010000026        34274 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.026, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000026 DAPHBJ010000000
VERSION     DAPHBJ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.026"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            80..1078
                     /locus_tag="RHZ76_001169"
     CDS             80..1078
                     /locus_tag="RHZ76_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HDV2811841.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(1075..1293)
                     /locus_tag="RHZ76_001170"
     CDS             complement(1075..1293)
                     /locus_tag="RHZ76_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HDV2811842.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(1295..3310)
                     /gene="ligA"
                     /locus_tag="RHZ76_001171"
     CDS             complement(1295..3310)
                     /gene="ligA"
                     /locus_tag="RHZ76_001171"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDV2811843.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(3381..4367)
                     /gene="zipA"
                     /locus_tag="RHZ76_001172"
     CDS             complement(3381..4367)
                     /gene="zipA"
                     /locus_tag="RHZ76_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HDV2811844.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPHPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            4597..5358
                     /gene="cysZ"
                     /locus_tag="RHZ76_001173"
     CDS             4597..5358
                     /gene="cysZ"
                     /locus_tag="RHZ76_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HDV2811845.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            5543..6514
                     /gene="cysK"
                     /locus_tag="RHZ76_001174"
     CDS             5543..6514
                     /gene="cysK"
                     /locus_tag="RHZ76_001174"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDV2811846.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            6898..7155
                     /gene="ptsH"
                     /locus_tag="RHZ76_001175"
     CDS             6898..7155
                     /gene="ptsH"
                     /locus_tag="RHZ76_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HDV2811847.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            7200..8927
                     /gene="ptsI"
                     /locus_tag="RHZ76_001176"
     CDS             7200..8927
                     /gene="ptsI"
                     /locus_tag="RHZ76_001176"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HDV2811848.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            8968..9477
                     /gene="crr"
                     /locus_tag="RHZ76_001177"
     CDS             8968..9477
                     /gene="crr"
                     /locus_tag="RHZ76_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDV2811849.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(9520..10371)
                     /gene="pdxK"
                     /locus_tag="RHZ76_001178"
                     /gene_synonym="thiJ"
     CDS             complement(9520..10371)
                     /gene="pdxK"
                     /locus_tag="RHZ76_001178"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HDV2811850.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVVTSASGNEENQEMQVVVVTADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALTDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            10476..10844
                     /locus_tag="RHZ76_001179"
     CDS             10476..10844
                     /locus_tag="RHZ76_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HDV2811851.1"
                     /translation="MKKIICLVITLLMTLPVYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(10847..11758)
                     /gene="cysM"
                     /locus_tag="RHZ76_001180"
     CDS             complement(10847..11758)
                     /gene="cysM"
                     /locus_tag="RHZ76_001180"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HDV2811852.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTDGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(11892..12989)
                     /gene="cysA"
                     /locus_tag="RHZ76_001181"
     CDS             complement(11892..12989)
                     /gene="cysA"
                     /locus_tag="RHZ76_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HDV2811853.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(12979..13854)
                     /gene="cysW"
                     /locus_tag="RHZ76_001182"
     CDS             complement(12979..13854)
                     /gene="cysW"
                     /locus_tag="RHZ76_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HDV2811854.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMICIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(13854..14687)
                     /gene="cysT"
                     /locus_tag="RHZ76_001183"
     CDS             complement(13854..14687)
                     /gene="cysT"
                     /locus_tag="RHZ76_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HDV2811855.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLILLFSINTLQSRFGRRVVG
                     H"
     gene            complement(14687..15703)
                     /gene="cysP"
                     /locus_tag="RHZ76_001184"
     CDS             complement(14687..15703)
                     /gene="cysP"
                     /locus_tag="RHZ76_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HDV2811856.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(15861..16652)
                     /gene="ucpA"
                     /locus_tag="RHZ76_001185"
     CDS             complement(15861..16652)
                     /gene="ucpA"
                     /locus_tag="RHZ76_001185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HDV2811857.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLSEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(16781..17638)
                     /gene="murR"
                     /locus_tag="RHZ76_001186"
     CDS             complement(16781..17638)
                     /gene="murR"
                     /locus_tag="RHZ76_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HDV2811858.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            17802..18698
                     /gene="murQ"
                     /locus_tag="RHZ76_001187"
     CDS             17802..18698
                     /gene="murQ"
                     /locus_tag="RHZ76_001187"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDV2811859.1"
                     /translation="MQFEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCSAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            18702..20126
                     /gene="murP"
                     /locus_tag="RHZ76_001188"
     CDS             18702..20126
                     /gene="murP"
                     /locus_tag="RHZ76_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HDV2811860.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            20131..21435
                     /gene="pbp4b"
                     /locus_tag="RHZ76_001189"
     CDS             20131..21435
                     /gene="pbp4b"
                     /locus_tag="RHZ76_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HDV2811861.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYYQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFNAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMTIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(21493..22392)
                     /gene="yfeX"
                     /locus_tag="RHZ76_001190"
     CDS             complement(21493..22392)
                     /gene="yfeX"
                     /locus_tag="RHZ76_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HDV2811862.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWCALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(22488..23063)
                     /locus_tag="RHZ76_001191"
     CDS             complement(22488..23063)
                     /locus_tag="RHZ76_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HDV2811863.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKDDNVAMVINGEQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(23270..23719)
                     /locus_tag="RHZ76_001192"
     CDS             complement(23270..23719)
                     /locus_tag="RHZ76_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HDV2811864.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(23706..24131)
                     /locus_tag="RHZ76_001193"
     CDS             complement(23706..24131)
                     /locus_tag="RHZ76_001193"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HDV2811865.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGDVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            24345..25214
                     /gene="amiA"
                     /locus_tag="RHZ76_001194"
     CDS             24345..25214
                     /gene="amiA"
                     /locus_tag="RHZ76_001194"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HDV2811866.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDELLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            25218..26117
                     /gene="hemF"
                     /locus_tag="RHZ76_001195"
     CDS             25218..26117
                     /gene="hemF"
                     /locus_tag="RHZ76_001195"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HDV2811867.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGADPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDRCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(26123..27175)
                     /gene="eutR"
                     /locus_tag="RHZ76_001196"
     CDS             complement(26123..27175)
                     /gene="eutR"
                     /locus_tag="RHZ76_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HDV2811868.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKLHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRNQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     SEKPSLTLHQRMREWG"
     gene            complement(27221..27721)
                     /gene="eutK"
                     /locus_tag="RHZ76_001197"
     CDS             complement(27221..27721)
                     /gene="eutK"
                     /locus_tag="RHZ76_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HDV2811869.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALAAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(27734..28393)
                     /gene="eutL"
                     /locus_tag="RHZ76_001198"
     CDS             complement(27734..28393)
                     /gene="eutL"
                     /locus_tag="RHZ76_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDV2811870.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(28403..29290)
                     /gene="eutC"
                     /locus_tag="RHZ76_001199"
     CDS             complement(28403..29290)
                     /gene="eutC"
                     /locus_tag="RHZ76_001199"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HDV2811871.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(29311..29649)
                     /gene="eutB"
                     /locus_tag="RHZ76_001200"
                     /pseudo
     CDS             complement(29311..29649)
                     /gene="eutB"
                     /locus_tag="RHZ76_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
     gene            29650..29880
                     /locus_tag="RHZ76_001201"
                     /pseudo
     CDS             29650..29880
                     /locus_tag="RHZ76_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416937.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            30127..30900
                     /locus_tag="RHZ76_001202"
     CDS             30127..30900
                     /locus_tag="RHZ76_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2811872.1"
                     /translation="MNILPTAYAPRQGESLGKHSLQKIDAIPEEVRSAIDDGLNDIRF
                     NGYRWECNAKTGWARVSPISSPADAEPESPEQSQANARADLLALQGEYLSVTQETKSE
                     KKSATDIVNELRSKAAKTSQTAPQQEAPEEPEQAEAMQDKETQYQTEEKGIYCSYVCL
                     GAARKAFVRSGCENKAKATAWSVLSSIASAARKQNDGSYVSEVCQAKIADEWGMNRRT
                     VIRQMKILVELGLIEPYGEYVIGQHARKYRMLFQVENAR"
     gene            32287..32478
                     /locus_tag="RHZ76_001203"
     CDS             32287..32478
                     /locus_tag="RHZ76_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV2811873.1"
                     /translation="MNDSELMTVNEVAALFGVTRRTIFRWMNKIKGWPVPVSPIGSRI
                     KFIRSEILEFYKNKGARHQ"
     gene            32618..32881
                     /locus_tag="RHZ76_001204"
     CDS             32618..32881
                     /locus_tag="RHZ76_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2811874.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFQEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKARLLFELSRRFGVSISTFK"
     gene            32899..33441
                     /locus_tag="RHZ76_001205"
     CDS             32899..33441
                     /locus_tag="RHZ76_001205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2811875.1"
                     /translation="MDAFDVLTNKGDELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIEIIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPTTDEYFSPD
                     AEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     AADPEAEQRKRVFNAIGIRY"
     gene            33580..33867
                     /locus_tag="RHZ76_001206"
     CDS             33580..33867
                     /locus_tag="RHZ76_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2811876.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            33906..34112
                     /locus_tag="RHZ76_001207"
     CDS             33906..34112
                     /locus_tag="RHZ76_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2811877.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
BASE COUNT         8442 a         9099 c         8656 g         8077 t
ORIGIN      
        1 catgaatgga tgcaataaat caattttatt tctcaaactg gaagaagcac aatagaacca
       61 tcgatcatct ggagtcttta tgaaactttt tcgtatcctc gatcctttca ccttaaccct
      121 gatcacggtg gtgttgctgg cctctttctt tccggccaga ggcgatttcg tccccttctt
      181 tgaaaatctg accaccgcag ctattgccct gctgttcttt atgcacggcg cgaagttgtc
      241 gcgtgaggcg attattgctg gcggtggtca ctggcgactg catttgtggg taatgtgcag
      301 caccttcgtg ctgtttccga ttctgggtgt actgtttgcc tggtggaaac cggtaaatgt
      361 cgacccgatg ctctactccg gttttctcta cttgtgcatt ctcccggcta ccgtgcagtc
      421 tgcaatcgcc ttcacgtcaa tggcgggcgg taacgtcgcg gcagcggttt gttctgcgtc
      481 ggcatccagc ctgctgggga ttttcctttc accattgctg gttggtctgg tgatgaatgt
      541 tcacggtgca gggggcagcc ttgagcaggt cggtaaaatt atgctgcaac tgctgctgcc
      601 gtttgtgttg gggcatcttt cccggccgtg gattggtgac tgggtgtcgc gcaataaaaa
      661 atggattgcg aaaactgacc agacgtccat tctgttggtg gtttatacag cgttcagcga
      721 agccgtcgtt aatggtatct ggcataaagt tggctgggga tcattgctgt ttatcgtggt
      781 ggtcagctgc gttcttctgg ctatcgtgat tgtagttaac gtctttatgg cacgccgact
      841 gagcttcaat aaggcagatg aaattactat cgtcttttgt ggttcgaaaa agagtctggc
      901 aaatggcatc ccgatggcaa acattctgtt ccccacatcg gtgatcggta tgatggtgct
      961 gcccctgatg attttccatc agatccaatt gatggtctgt gcggtgctgg cgcgtcgata
     1021 caaacgccag accgaacagt tacaggcgca gcaggaaagc agcgccgata aagcttaaag
     1081 cggacgcttc aggggctgga ccagttgcgt cagcccctcg gttttaatca gcagcgtgat
     1141 ttgcattagc tcaccgagct tccctgccgg gaactcatct ttgcgggcaa accacagcag
     1201 atactcctcc ggcaggtcga ttaagcgacg ccctttgtat ttgccaaacg gcattatcgt
     1261 attggctatt tcaatcagct gctctttttc catctcagct acccagcaaa cgcagcattt
     1321 ccgcttcgtc gatgacttca atgcccagtt cctgcgcttt cgccagttta gaacctgccg
     1381 cttctccggc aatcaccaga tcggttttct tcgacacgct gcccgcgact ttcgcgccca
     1441 gttcaaccag tcgagcttta gcgtcatcac gcgacatctg gcttaagctg cccgtaagca
     1501 ccacggtttt accagcaaac gggctgtcaa tctcttccgc gttgataacg atcggcgcag
     1561 gccagtgaac accttccgcc aacagctcgc tgatgacatt gcggttgctt tcttcggcaa
     1621 agaagttgtg aacgtgggat gcaacgacaa tgccaacatc aggcaccttt tgcagctctt
     1681 caatcgaagc ggcttccagc gcttccagcg tgccgaaata tgccgccaga cctgctgcgg
     1741 tggcctcgcc gacttcacgg atgccaagtg catagaggaa gcgagcaaag gtggtttctt
     1801 tcgctttttc cagcgcgtta accacgtttt gtgccgattt tggccccata cgctccagtc
     1861 cggtcagttt gcctgcggtg agtttgaaca gatctgccgg agtatggaca tattcttttt
     1921 caaccagctg atcgatgatt ttgtcgccca ttccgtcaac atccatcgca cggcgggaaa
     1981 caaagtgttt cagcgactct ttacgctgcg caccgcaaat caggccaccg gtacagcggg
     2041 caaccgcttc accttccaca cgctcaacgt cagaaccaca taccggacaa tgcgtcggga
     2101 atacaacctc acgggtatct tccgggcgtt cagaaagcac gacgttaacc acctgcggga
     2161 tcacgtcgcc agcgcggcga atcaccactt tatcgccaat gcgtaaacca agacgttcga
     2221 tttcatccgc attgtgtaag gttgcgttac tcaccagcac gccagcaaca tggacaggtt
     2281 ccagacgcgc aacaggcgta atcgcgccag tacgcccaac ctgaaactcg acgtcacgca
     2341 caaaggtcat ctgctcctgc gccgggaatt taaacgctac cgcccagcgc ggggcacgcg
     2401 cgacaaagcc aagctgctcc tgctgtgcca gtgagttgac cttaatcacc acgccgtcga
     2461 tatcaaagcc cagcgtcggg cggtcttctt ccactttgtg atagaacgcc agcacttctt
     2521 ccgccgattc acaaagcgtt acccgatcgc tgaccggcaa cccccacttt ttaaattgca
     2581 gtaaacggcc aagatgagta tccggcagct cgccaccttc cagaacacca acgccatagc
     2641 agaaaaaagt gagcggtcgc ttcgctgtaa tacgcggatc aagctgacgc agtgaaccag
     2701 ctgccgcatt acgtgggtta gcaaaaactt tcccgcccgt gcgtcgcgca tcttcgttaa
     2761 tcttttcgaa ccccgcctgc ggcaggaaca cttcaccacg cacttccaga cgcgccggga
     2821 tattctctcc gtgcagcttc agcggaatgg cgcgaatagt acgcacatta gacgtgatat
     2881 cttccccggt ggtgccatcg ccacgggtcg cggcactgac taaaacgcca ttttcataca
     2941 gaatactgac ggcaaggcca tccagcttca gctcacagca ccaggtgact ttctcgttgt
     3001 ttttcagacg gtcctgcaca cgtttgttga aagcaagaaa gctttcttca tcaaaaacgt
     3061 tatccagtga cagcattggt acttcatggc gtatctggct gaaagccgcc agcggcgcag
     3121 cgcctacacg ttgagtaggc gaatcaggcg taatcagttc tggatgtttg gtttccagct
     3181 cgcgcagttc gcgcatcagc ctgtcgtatt cagcgtcggg aatttccggc gcatccatca
     3241 catgataaag atattcatga tggcgaagcg tcgttcgcag ttctgtcagt tgttgttcga
     3301 ttgattccat atcgcaccat caatgctaaa aacccccgac aagcgggggt tcgaagagga
     3361 gttaatttgc cttaagtgta tcaggcgttg gcgtctttga cttcgcggat gatgtcctgg
     3421 tactcgcgca atttctgcgg agtcatcata cggcgctgat cgtcaagcac gacaccgccc
     3481 acttcatcgg caatatgctg cgcagattgc agcatcagct tgaagttctg cagctcgtca
     3541 ccgtaagacg gtacctgcat aaagatagtg acacccggag tagtgaaatc cttcatttca
     3601 ggatcaaagg ttcccggttt caccatattc gccaggctga ataacgccgg gccgctgcca
     3661 tccgggctaa gatgacgatg gtaaatattc atatcgccaa aaatgaagcc cgcttgttga
     3721 atgctgttaa gaagcagttc accgtttagc tcgctaccgt gatgcgccgc gacgttcatg
     3781 ataatcaccg cttctttgcg cttcggttta tccataactg gagcaggttc cgctacaggc
     3841 tcaggctgtg gtgccgctac gggttctgca ggctggaaag cctgttgtgc cggttgcggt
     3901 gctgaatgca caggctgcgg cgctggcgcg acctgtggcg aaactggctg ctgcaacggc
     3961 tgttcaggct gcggctgata ggcaggctgc tgcaccggct gcgctggatg cggagcatgt
     4021 tgcggcggta cctgcgcttc aggcggctgc tggaccggtt gacgcggctg cgcagacgca
     4081 taaggcggtt ggtactggtg ttgcggcgac ggacgagcag cctcatgctc ctgagcgtta
     4141 gccggggcat gattcacgcg gtgaacacga acctcaccaa cgccctcatc atcttcgaca
     4201 tcctcgtcat aagaatcgtc gtcacgtttt gacttcattc gttttaatgg ccgatcgcgg
     4261 aacatagaag atcgttcttt acggctggtc cagaaaccat gtaccagtaa agcgattatg
     4321 gcgatcgcgc caacaatgat taatatcaga cgcaaatcct gcatcattat attctctgtt
     4381 gttctaacac cttgccacca cggcaaacat ttactcacta agagtatttg ccgattacct
     4441 caagtgcaag tgcactatta actttcacag cacaaagata gatgaaatcg tgctttttgc
     4501 tgttttttcg aacatatcct aactgtccat tgcgcaatta cccggtaaaa tacgcagaat
     4561 tttcctggga ttggtcaaaa ggagctcatc ctgattatgg tttcatcatt cacatctgcc
     4621 ccacgcagcg gtttttacta ttttgcgcaa ggctggaagc tcgtctcgca acctgggatt
     4681 cggcgtttcg ttattttacc gctgctggtc aatattttgt tgatgggggg cgcattctgg
     4741 tggctcttta cgcaactcga tgtctggatc ccgactctca tgagttatgt tccggactgg
     4801 ttacaatggc tgagctatct gttgtggccc ctggctgtca tctctgtgct gttagtgttt
     4861 ggctatttct tctcaacgat tgctaactgg attgccgctc cgttcaacgg tttattggct
     4921 gaacaactgg aagcacgatt gactggcgct acaccgccag ataccgggat tttcggtatc
     4981 atgaaagatg tgccgcgaat catgaaacgc gaatggcaaa aatttgcctg gtatctgcca
     5041 cgcgcaattg tattgctaat tctttacttc atccctggta ttgggcaaac cgtcgcgccg
     5101 gtactgtggt tcctgtttag cgcctggatg ttagccatcc agtattgcga ttaccccttc
     5161 gataaccaca aagtgccgtt taaagagatg cgcaccgccc tgcgcacacg caaaatcacc
     5221 aatatgcagt ttggtgcttt aaccagcctg tttacgatga tcccgctgct taatctgttc
     5281 atcatgcccg ttgccgtttg tggcgcgacg gcgatgtggg tcgattgcta tcgcgataaa
     5341 cacgcgatgt ggcggtaaca atctaccggt tattttgtaa accgtttgtg ttaaacaggg
     5401 gtggcttatg ccgccactta ttccatcttg catgtcatta tttcccttct gtatatagat
     5461 atgctaaatc cttacttccg catattctct gagcgggtat gctacctgtt gtatcccaat
     5521 ttcatacagt taaggacagg ccatgagtaa gatttttgaa gataactcgc tgactatcgg
     5581 tcacacgccg ctggttcgcc tgaatcgcat cggtaacgga cgcattctgg cgaaggtgga
     5641 atctcgtaac cccagcttca gcgttaagtg ccgtatcggt gccaacatga tttgggatgc
     5701 cgaaaagcgc ggcgtgctga aaccaggcgt tgaactggtt gaaccgacca gcggtaatac
     5761 cgggattgca ctggcctatg tagctgccgc tcgcggttac aaactcaccc tgaccatgcc
     5821 agaaaccatg agtattgaac gccgcaagct gctgaaagcg ttaggtgcaa acctggtgct
     5881 gacggaaggt gctaaaggca tgaaaggcgc aatccaaaaa gcagaagaaa ttgtcgccag
     5941 caatccagag aaatacctgc tgctgcaaca attcagcaat ccggcaaacc ctgaaattca
     6001 cgaaaagacc accggtccgg agatatggga agataccgac ggtcaggttg atgtatttat
     6061 tgctggcgtt gggactggcg gtacgctgac tggcgtcagc cgctacatta aaggcaccaa
     6121 aggcaagacc gatcttatct ctgtcgccgt tgagccaacc gattctccag ttatcgccca
     6181 ggcgctggca ggtgaagaga ttaaacctgg cccgcataaa attcagggta ttggcgctgg
     6241 ttttatcccg gctaacctcg atctcaagct ggtcgataaa gtcattggca tcaccaatga
     6301 agaagcgatt tctaccgcgc gtcgtctgat ggaagaagaa ggtattcttg caggtatctc
     6361 ttctggagca gctgttgccg cggcgttgaa actacaagaa gatgaaagct ttaccaacaa
     6421 gaatattgtg gttattctac catcatcggg tgagcgttat ttaagcaccg cattgtttgc
     6481 cgatctcttc actgagaaag aattgcaaca gtaatgccag cttgttaaaa atgcgtaaaa
     6541 aagcaccttt ttaggtgctt ttttgtggcc tgcttcaaac tttcgcccct cctggcattg
     6601 attcagcctg tcggaactgg tatttaacca gactaattat tttgatgcgc gaaattaatc
     6661 gttacaggaa aagccaaagc tgaatcgatt ttatgatttg gttcaattct tcctttagcg
     6721 gcataatgtt taatgacgta cgaaacgtca gcggtcaaca cccgccagca atggactgta
     6781 ttgcgctctt cgtgcgtcgc gtctgttaaa aactggcgct aacaatacag gctaaagtcg
     6841 aaccgccagg ctagacttta gttccacaac actaaaccta taagttgggg aaatacaatg
     6901 ttccagcaag aagttaccat taccgctccg aacggtctgc acacccgccc tgctgcccag
     6961 tttgtaaaag aagctaaggg cttcacttct gaaattactg tgacttccaa cggcaaaagc
     7021 gccagcgcga aaagcctgtt taaactgcag actctgggcc tgactcaagg taccgttgtg
     7081 actatctccg cagaaggcga agacgagcag aaagcggttg aacatctggt taaactgatg
     7141 gcggaactcg agtaatttcc cgggttcttt taaaaatcag tcacaagtaa ggtagggtta
     7201 tgatttcagg cattttagca tccccgggta tcgctttcgg taaagctctg cttctgaaag
     7261 aagacgaaat tgtcattgac cggaaaaaaa tttctgccga ccaggttgat caggaagttg
     7321 aacgttttct gagcggtcgt gccaaggcat cagcccagct ggaaacgatc aaaacgaaag
     7381 ctggtgaaac gttcggtgaa gaaaaagaag ccatctttga agggcatatt atgctgctcg
     7441 aagatgagga gctggagcag gaaatcatag ccctgattaa agataagcac atgacagctg
     7501 acgcagctgc tcatgaagtt atcgaaggtc aggcttctgc cctggaagag ctggatgatg
     7561 aatacctgaa agaacgtgcg gctgacgtac gtgatatcgg taagcgcctg ctgcgcaaca
     7621 tcctgggcct gaagattatc gacctgagcg ccattcagga tgaagtcatt ctggttgccg
     7681 ctgacctgac gccgtccgaa accgcacagc tgaacctgaa gaaggtgctg ggtttcatca
     7741 ccgacgcggg tggccgtact tcccacacct ctatcatggc gcgttctctg gaactacctg
     7801 ctatcgtggg taccggtagc gtcacctctc aggtgaaaaa tgacgactat ctgattctgg
     7861 atgccgtaaa taatcaggtt tacgtcaatc caaccaacga agttattgat aaaatgcgcg
     7921 ctgttcagga gcaagtggct tctgaaaaag cagagcttgc taaactgaaa gatctgccag
     7981 ctattacgct ggacggtcac caggtagaag tatgcgctaa cattggtacg gttcgtgacg
     8041 ttgaaggtgc agagcgtaac ggcgctgaag gcgttggtct gtatcgtact gagttcctgt
     8101 tcatggaccg cgacgcactg cccactgaag aagaacagtt tgctgcttac aaagcagtgg
     8161 ctgaagcgtg tggctcgcaa gcggttatcg ttcgtaccat ggacatcggc ggcgacaaag
     8221 agctgccata catgaacttc ccgaaagaag agaacccgtt cctcggctgg cgcgctatcc
     8281 gtatcgcgat ggatcgtaaa gagatcctgc gcgatcagct ccgcgctatc ctgcgtgcct
     8341 cggctttcgg taaattgcgc attatgttcc cgatgatcat ctctgttgaa gaagtgcgtg
     8401 cactgcgcaa agagatcgaa atctacaaac aggaactgcg cgacgaaggt aaagcgtttg
     8461 acgagtcaat tgaaatcggc gtaatggtgg aaacaccggc tgccgcaaca attgcacgtc
     8521 atttagccaa agaagttgat ttctttagta tcggcaccaa tgatttaacg cagtacactc
     8581 tggcagttga ccgtggtaat gatatgattt cacaccttta ccagccaatg tcaccgtccg
     8641 tgctgaactt gatcaagcaa gttattgatg cttctcatgc tgaaggcaaa tggactggca
     8701 tgtgtggtga gcttgctggc gatgaacgtg ctacacttct gttgctgggg atgggtctgg
     8761 acgaattctc tatgagcgcc atttctatcc cgcgcattaa gaagattatc cgtaacacga
     8821 acttcgaaga tgcgaaggtg ttagcagagc aggctcttgc tcaaccgaca acggacgagt
     8881 taatgacgct ggttaacaag ttcattgaag aaaaaacaat ctgctaatcc acgagatgcg
     8941 gcccaattta ctgcttagga gaagatcatg ggtttgttcg ataaactgaa atctctggtt
     9001 tccgacgaca agaaggatac cggaactatt gagatcattg ctccgctctc tggcgagatc
     9061 gtcaatatcg aagacgtgcc ggatgtcgtt tttgcggaaa aaatcgttgg tgatggtatt
     9121 gctatcaaac caacgggtaa caaaatggtc gcgccagtag acggcaccat tggtaaaatc
     9181 tttgaaacca accacgcatt ctctatcgaa tctgatagcg gcgttgaact gttcgtccac
     9241 ttcggtatcg acaccgttga actgaaaggc gaaggcttca agcgtattgc tgaagaaggt
     9301 cagcgcgtga aagttggcga tactgtcatt gaatttgatc tgccgctgct ggaagagaaa
     9361 gccaagtcta ccctgactcc ggttgttatc tccaacatgg acgaaatcaa agaactgatc
     9421 aaactgtccg gtagcgtaac cgtgggtgaa accccggtta tccgcatcaa gaagtaattc
     9481 ttgccgcagt gaaaaatggc gcccatcggc gccatttttt tatgcttccg ccagcggcgg
     9541 caaaatcaat tcatcgctct catgctgctg ggtgtagcgc atcacttcca gtacgcgcaa
     9601 ccccgctcgg tgcactgcat cggttaacgc cttccctttc agcaagccac tgatgagctg
     9661 agcacaaaac aggtcgccag tccctttcag gtcggttttt acccgtgaat gggaaatgac
     9721 attcacgctg tcggcagtga ccaccacaac ctgcatctcc tgattttctt cattaccgga
     9781 ggcgctggta accaccaccc attttaatgt gtctgaaagc agactttttg cggcagcaat
     9841 ggcactgtcg agatcgcggc aatttttacc ggtcaggatt tccaactcaa agatattggg
     9901 ggtaattccc tgcgccagcg gcagtaaata ttgtcgatac gcttcgggaa ggtcaggttt
     9961 gacataaatt ccgctatcaa tatcgccaat caccggatcg accatgatca ataggtcagg
    10021 atggtctttg cgtagcgcag tcagccactc ggcaaggatt ttgatttgcg atgccgttcc
    10081 catatagccc gtggttacag cacgaagttg gcgcagcgca tcacgctcct gaagcgcacg
    10141 caaatagccg ctaaaccatt cgtccggaat cgcaccaccg tagaaagtgt cataatgcgg
    10201 cgtattgctc agcaataccg tcggcacggc aaagacattc aggccgttct gtttgatagc
    10261 aggcacggca atgctgttgc ccacgctgcc gtaaaccacc tgcgactgca cggcgacgat
    10321 atccgcctgc agtgccctac tcttatcgtt aaacaacaac aaactactca tttaattttt
    10381 tctccttgcc gatgatcctc atcgtaatcc aaccgaaact ttacctgatt ctggcagtca
    10441 aatcggctat cacaaaacaa ggataaggta attcaatgaa gaaaatcatt tgtctggtca
    10501 ttacactact aatgacactc cccgtttacg cgaagttaac tgcccatgaa gaagcccgca
    10561 tcaacgccat gctggaggga ttagcacaga aaaaggattt gatatttgtg cgcaacggtg
    10621 atgaacatac ctgcgatgaa gcggtttctc atctgcgtct gaagctgggc aatacccgta
    10681 accgcattga cactgccgag cagtttattg ataaggttgc ttcgtcgtca tcgattaccg
    10741 ggaagccgta tattgtgaag atccccggta agagcgatga gaacgcacag ccttttttac
    10801 atgcgttaat tgcgcagacg gataaaacag tgcctgcgca ataatcttaa atccccgccc
    10861 cctgactaaa atgctcttcc ccaaacaccc cggtggaaag gtagcgatcg ccacgatcgc
    10921 agatgatcgc taccaccacc gcaccagggt tagcttttgc cacccgcagt gctccggcaa
    10981 ccgcgccgcc ggagctgacg ccacagaata ttccttcccg caccgccagt tcgcgcatgg
    11041 tgttttccgc atcgcgctga tgaatatcca gcacctcatc caccagagaa gcgttgaaaa
    11101 tccccggcag atattccgca ggccagcggc gaatgccggg aatgctactg ccctcttccg
    11161 gctgcaggcc aacaatggtc accggtttgg attgttcgcg cataaagcgt gaaacgccgg
    11221 tgatagtgcc ggttgttccc atgctggaga caaaatgggt aatgcgcccg tcagtttgct
    11281 gccagatttc cggcccggta gtggtgtaat gggcgtaagg gttatcggga ttattgaact
    11341 gatcgagcag ctttccttca ccacgattag ccatctccag cgccagatcg cgcgcacctt
    11401 ccatgccctg ctctttggtg acaagaatca gttccgcacc ataagcacgc atcgccgcac
    11461 ggcgttcctg gctcatgttg tcgggcatca gcaatttcat gcgatagcct ttaagcgcgg
    11521 caatcattgc cagcgcaatg ccggtgttac cactggtggc ttcgattaac acatcacccg
    11581 gtttaatttc cccgcgcttt tccgcctcga cgatcatcga aagcgccgca cgatctttta
    11641 ccgaacctgc cgggttattg ccttccagtt ttaaccacac ttcactgccg ttattcggcc
    11701 ccattcgctg caacttcacc agaggcgtat tgcctattgt ttgttctaat gtactcacga
    11761 tctctatcca tacgtggtgt tgcctgatgc gacgcttgcg cgtcttatca ggcctacagg
    11821 ttacaaacct tgccattaaa aagcccggat cgcgggaagc gcctccgggc gtttaacatt
    11881 cactcaacct atcaggcgct ttgtgcgaga gcaagttcct catcgcgggt ttcgatacgc
    11941 tcgtcgccgt tatacagccg cgcatgttgc agaccaacga ataaacgctc gccacgctgc
    12001 ggggcatcgt cgccatgcat cacgaccgtc agcggttcgt tgtaccaccc cagcggctgc
    12061 accactaatt gggtgtagtg acctttcggg ctggcttcca gtacctgtac cggcagcggc
    12121 gaatcgaggc tggtacggcg gctgatatcc acttcccaag ggcgcaggaa gagatccacc
    12181 ggcccctgat acgcaggtgt gtagcccagc ggccagcgat gcgcgccaac atggaactgc
    12241 ccgccgcgaa tggttccctg caggcggttc acttcgccca taaattcgag cacaaaacgg
    12301 gtcgccggtt cgcgccatac ctgatccggc gcgtcagcct gttcaatatt gccctggctc
    12361 atcacaacta cacgatcagc tacttcggtc gcttcttcct gatcgtgggt cacaaaaacg
    12421 ctggtgaatt ttagttcttc atggagttga cgcagccagc gacgcagctc tttacgcacc
    12481 tgcgcatcca gcgcgccaaa cggttcatca agcagcagaa tttgcggttc cacagccagc
    12541 gcgcgcgcca gcgccacgcg ctgtttctgg ccgccggaaa gctgcgccgg ataacgatcc
    12601 gccagatggg caagctggac catttccagc aattttgtca ctttcgcttt gatggctgcg
    12661 gcattcgggc gctcgcgacg cggcagcacc gtcaggccaa aagcgatatt gtcgaacacc
    12721 gtcatatggc ggaacagcgc gtaatgctgg aacacgaaac cgactttacg atcacgtgcg
    12781 tgcaggcggc tcacgtcggt gccgtggaag cgaatatgcc cgctggtttg atgctccagc
    12841 ccggcgataa tgcgcagcag cgtggttttc ccggaaccgg acggccccag caacgcgacc
    12901 atctgacctg aaggaatatc cagtgagata tcgttcagca cctgggtgcg accaaacgac
    12961 ttcttaatat tggcaatctc aatgctcatg atgttcctcc tgctgtgcgc gtttttcctg
    13021 attctccagg cgccactgca acatactttt taaaaacagg gtgataatcg ccatcagcgt
    13081 taacagcgcc gcagcggtaa aggagccgac ggtgttgtag tcctgctcca gcaattcaat
    13141 ctgtaacggc agcgacaggg tttcgccgcg aatcgagccg gaaaccaccg acactgcgcc
    13201 aaactcgcca atcgcgcggg cgttggtcag caccacgcca taaagcagcg cccagcgaat
    13261 gttcggcaat gtgacgcggc gaaacatctg ccagccagac gcgccaagca aaattgccgc
    13321 ttcgtcttcc tggctgccct ggcttaacat caccggcacc agctcgcgca ccacaaacgg
    13381 acacgtcacg aagatcgtga ccagcaccat tccgggccaa gagaacataa tttgcagatt
    13441 atgttcatcc agccagccgc cgagcgggcc gttggagccg tagaacagca gatacaccag
    13501 accggcaacc actggcgata cggcaaaagg aatgtccagt agcgtcagca gtaactggcg
    13561 tccggggaag ttaaagcgcg tcaccagcca ggccagcaga atgccgaaca ccaggttcac
    13621 cggtacggca atcagcgcaa tcatcaccgt cagccaaatc gcgtgcaaca tgtccggatc
    13681 ggccagattc tgtaaaaccg gcatcagccc cttgctgaat gcctgcacga agatgcaaat
    13741 catcggcacc agcaggatga acgccgaaac cagcatcccg atgccaatta gaaaccattt
    13801 gccccagtta atcgggcgcg cgtcataacg cttcaattgt gtcacttccg ccattaatga
    13861 cctaccacac gccgaccaaa gcgactttgc agagtgttaa ttgagaacag cagtatcagg
    13921 gatgccgcga ggatcaccga agcaatcgcg ctcgccgccg ggtaatcaaa ctcctgcaag
    13981 cgcacaaaaa tcatcagcga cgtcacttcc gtcttccacg cgatatttcc ggcgataaaa
    14041 atcaccgcgc caaattcacc aagactacgg gtaaacgaca gcgccacgcc cgccaccagc
    14101 gccggagaaa gctccggcag caccactttg cagaaactct gccagcgcgt tgcaccaagt
    14161 gtttccgccg cttcttcata ttccgggcct aactcttcca gcaccggctg cacggtacgt
    14221 accacaaacg gaatgctggt aaaggccatc gccaccgcaa tccccagcca ggtataggtg
    14281 actttgatat caaacttcgc cagccattca ccgtaaaaac cgtttacaga aaagagcgag
    14341 gccagcgtta aaccggcgac tgccgttggc agcgcaaagg gtaaatccat cagcgcatca
    14401 agcagcgtgc ggccagggaa gcgataacga gttaagatcc acgccatcag cagaccgaaa
    14461 acgccgttaa aaatcgatgc cacaaacgcc gacagcagcg tcactttgta ggctgcgacc
    14521 acctgcgggt tggtgatcac ctcccagtac tgcgcccagc tcatctgggc cagctgcatc
    14581 accagcgcgg agagtggcag cagcaaaatc aggcaaacaa acagcagact ggtgccgagg
    14641 cttaaggtaa agcccggcag cacgcgtctt gaagaaaccg caaacattaa ttacgccccg
    14701 ccgctaacag cttgtctaac tcgccgccgc tggtgaagtg ggttttcatc acttccggcc
    14761 aggagccaaa tttgtcttcc acgcggaaca gctcggtctg cgggaattta tctttcagtt
    14821 tgtccatgac ttccgggtta ttcacgcggt agtaatagtc ggtgatgatg gtttgcgcct
    14881 gcgggctgta gagccagttc aggtaggctt tggcggcttt ttccgtaccg ttggcctgca
    14941 cgtttttatc gacccacgcc accgggaatt ccgccagaat gttggttttc ggaatcacca
    15001 cttcaaagcc ctgcgcttca tactgtttgc ggatgttgtt cacttccgac tcgaagctga
    15061 tcagcacatc gcccaggccg cgctcggcga aggtggtggt cgcgccacga ccgccggtat
    15121 cgaacacttc aacgtttttc aggaactgag tcataaactg ttcggttttg gctttgtcgc
    15181 caccgtcagc tttgtccgct gcgccccatg ccgccagata ggtataacgc gcgttacccg
    15241 acgttttcgg gtttgggaaa atcagcttca cgtcggagcg caccaggtcg ttccagtcgt
    15301 ggatattctt cgggttgccc ttacgcacca ggaagcccat ggtggagtag aacggcgagc
    15361 tgttattcgg caggcgcgac tgccagtcgg ccgggatcag cttgcctttg tcatgcagga
    15421 tttgtacgtc ggtcacctgg ttataagtga caacgtctgc ttttaagccc tgcaaaattg
    15481 ccagcgcctg ttttgatgac ccggcatgag attgttttat cgtcagtttg tcaccgccgt
    15541 tatctttggc ccattgttgc tcaaacggcg ggttcagggc ggcaaacagc tcgcgggaga
    15601 cgtcataaga actgttcagc agttccgttg cctgtacatg gcccgccagc agcagagaag
    15661 cgaccagcgc gagtgagttc tttttcagta agttaacggc cattgcgcac ccttataaat
    15721 ttaatgactt tctaatagcc atcatattta taacggacgt gaaaggagta acggttttat
    15781 ataccgtttg gtgatttgga agttgaaaag ggaataagac gaaatcctcc ccacaaatgc
    15841 agggaggaaa cagaggtgaa tcagataccg acgctaaccg tctccggcag tgtgctgccg
    15901 ccatcaatca cattctgtgt accggttaaa tagctggatt catccgatgc gaggaaggcc
    15961 gccagttcgc cgacttccag cggatcggcg aggcgacgca tcgggattgc tttcgccatt
    16021 tcagtcagca ccgactctgg atcttccggg ttcgactggc gggcaatgct ttccgccatt
    16081 ggtgtgcgca cgtatcccgg gcaaatggcg ttaacgcgaa taccagactg cgcgtactcc
    16141 accgccagcg attttgtcag gccaacaatc gccgctttcg ttaaggcgta cgccgtttcg
    16201 ccaggatcgg ccaccatatc accagtgact gaagacatca tcacaatgcg accatctttg
    16261 cgggcaatca tctccgacag caccgccttc gtgacgttcc atacgccttt aatattgatg
    16321 tcaatatgga aatcgcgatc gtcatcgctc atatcgagga aactgcccag acgacaaacg
    16381 cctgcgttat tcaccaggat atcaatgcgc ccttcttttt ccttcgcgcg tttgatagct
    16441 gcggctaccg acgccgggtc acgcacatcg gcgacaaccg ccgtacagcg atgaccacga
    16501 ccacacagtt cgtccgccag cttttcgatc tcaggggaga tatccagcaa gattaggttt
    16561 gcgccatgac gcgcaaaagt tctggcaatt ccttcgccaa ttccctgcaa tgcgcccgta
    16621 atcagtgctg tcttgcccgt gagtttaccc attttaatgc tctccttgtt aatacagagc
    16681 gttacactcc attccctctg aaaatacagg ataaacaaaa tattgctgcg gatcagctca
    16741 ctaattcagg aaaatctcaa tccagtcgct gctttatgca tcatttcagg cgttgtgtta
    16801 gctcactgct gcgctgaatc attttcaatg actccacatc attgagttgc acaagaccaa
    16861 caaacagtaa atcggtaaca gaattttgcg ccgtgcgggt agacatcgac gagctacgcc
    16921 attcggtttc accggaaacg gtatcgagcg tgaaatgcgc cagccgccgc agcggagagt
    16981 cagccagaga agtaatggca atcaccgttg ccccctgctt tcgtgccgct tctgcgcaca
    17041 agacaatctc ttttttactg ccgctgtaag agatagcgat ttgtacatcg ccttttttca
    17101 acgcctggga aacggtggct tgtacgtgcg tgtcggcttc acaggccacg cgatagccaa
    17161 ttttcatcag tttgaacgac agatcgcgcc ccaccagcgc cgacccgccc aggcccgtta
    17221 tctggataaa cggtgcttta ctgatgacct caatgatttt ttgcagccgc gcgtagtcga
    17281 acaacgcaca ggtttgctcc agcgccagct ctttttcgcg attcagtttg cgggcaatca
    17341 cttccagcga atcatcactg gtaatcgaac tgtgcaggtg cagcgccgtg gcattcgttt
    17401 tctcccggct ggcactgtat tcgcctatta acgccatacg caattcagta aatccttgcg
    17461 cgccgagttt ttgggcaaat ttcactatgc tcgactggct aatgccgagc tgtttcgcca
    17521 tctgccgtga agaaacggat tgcagctcac tcacgttggc ccgcagaaag tcagcaattt
    17581 tttgctcgtt ctcggtaaat tcactttccg cattgcgtat tttcgtcagg tacaacatct
    17641 tccactctcc tgggctggca gcaaaaattc cgcttaagtt cttatgacgc tcttcacact
    17701 ctgcgagtgt agactcaatg attcctttaa gtctgtcaat tcgggatata tgattccata
    17761 catctgcaat tatttgatcg taaatagtaa ggtcaccacc gatgcaattt gaaaagatga
    17821 ttactgaagg ctcgaacacc gcctcggctg aaattgaccg cgtatcgacg ctggaaatgt
    17881 gccggattat caacgatgaa gataaaaccg taccgcttgc cgttgagcgc gtactgccgg
    17941 atatcgccgc ggcgatcgat gttatccacg cccaggtaag cggcggcggg cgtctgattt
    18001 acctcggtgc gggaacatcc ggtcgtctgg ggattctgga tgccagcgaa tgtccgccca
    18061 cctacggcgt gaaaccgggt ctggtggttg gtttgattgc tggcggcgaa tatgccattc
    18121 agcacgcggt ggaaggcgcg gaagatagcc gggaaggcgg tgttaatgat ctgaaaaata
    18181 ttaatttaac ggcacaggat gtggtggttg gcattgctgc cagcggtcgc acgccgtatg
    18241 tgattgccgg actggaatac gcacgccagc tcggctgccg cacagtggga atttcctgta
    18301 atccggggag cgccgtttca accaccgctg agtttgccat tacaccgatt gtaggtgccg
    18361 aagttgttac cggttcttcg cggatgaaag caggtacagc gcagaaactg gtgctcaata
    18421 tgctttccac cgggctgatg attaaatccg gcaaagtgtt cggcaacctg atggtcgatg
    18481 tggtcgccac caacgaaaaa ctgcatgtgc gccaggtcaa tattgttaaa aacgccaccg
    18541 gatgtagcgc agagcaagcg gaagcggcgt taattgcttg cgagcgcaac tgtaaaacgg
    18601 ccattgtgat ggtgctgaaa aatctcgatg ccgcagaagc taaaaaacgc ctggatcaac
    18661 acggcggctt tattcgtcag gttttagaca aggaataacc catggccaaa gagatcagca
    18721 gtgaacttct gaacaccatt cttacccgtg tcggcggacc gggaaatatc gccagttgtg
    18781 gtaactgtat gacgcgcctg cgtctgggtg tacatgacag ttcactggtt gatcccaata
    18841 tcaaaacgct ggaaggcgtg aagggcgtca ttttgaccag cgaccaggtg caggtcgttt
    18901 ttgggcctgg taaagcacat cgtgccgcaa aagcgatgag cgagctgctg ggcgaggcac
    18961 cggtacagga tgccgcagaa atcgccgccc agaacaaacg tcagttaaaa gccaaacaaa
    19021 cctccggcgt gcaacaattt ctcgccaaat tcgccaccat cttcacgccg ctgatccccg
    19081 gttttattgc cgccggtctg ctgctgggga tagcgacgtt aattgccacg gtgatgcacg
    19141 ttccggcaga tgctcaggga acactacccg atgcgctgaa ttttatgaag gtgttcagca
    19201 aaggtttgtt cactttcctg gtgattctgg tgggctataa cgccgcccag gcatttggcg
    19261 gcacgggcgt aaatggcgca attatcgccg cgctcttttt gctcggttat aaccccgcag
    19321 cgaccaccgg ttactacgcc ggttttcacg atttctttgg tctgcccatc gatccgcgcg
    19381 gcaatattat cggcgtgctg attgccgcgt gggcctgcgc ccgcattgaa ggcatggtgc
    19441 gccgctttat gccggacgat ctcgacatgc tgctgacctc gttaatcacc ctgctgatca
    19501 ccgctacgct tgcgtacctg atcattatgc cgctgggtgg ctggttattc gaaggtatgt
    19561 catggctgtt tatgcacctg aacagtaatc cgttcggttg tgcggtttta gccgggctgt
    19621 tcctgatcgc cgtggtattt ggcgtgcatc agggctttat tcctgtttac ctcgcgttaa
    19681 tggacagcca gggattcaac agcttatttc ccatcctttc aatggcaggc gcgggccagg
    19741 tgggcgcggc gctggcactc tactggcggg cgcaaccgca cagtgcgcta cgcagtcagg
    19801 tacgcggggc gattattccc ggcctgctgg gcgttggtga accgctgatt tacggtgtca
    19861 ccctgccccg catgaagccg tttgttaccg cctgtttagg cggcgcggcg ggcggtttgt
    19921 ttatcggcct gatagcctgg tggggtctgc cgatgggctt aaacagcgcc tttgggccgt
    19981 ctggtctggt agcgctgccg ctgatgactt ccgcacaagg catccttccg gccatggcgg
    20041 tttatgctgg cgggattctg gtggcatggg tttgcgggtt tattttcacc acgctctttg
    20101 gctgccgtaa cgtcaatctg gactgatatc atgaaacgga caatgctcta tctttctctg
    20161 ctggctgtta gttgtagcgt cagcgccgca aaataccctg ttctgacaga aagctcgcca
    20221 gagaaagcag ggtttaacgt cgaacggctt aaccagatgg atcgctggat tagccagcaa
    20281 gttgatgtcg gttatcccgg cgtaaacctg ctgatcatta aagataatca gattgtgtat
    20341 cgcaaagcct ggggagcggc gaaaaagtac gatggcagcg tgttgatgga acagcctgtc
    20401 aaagccacca ccgggacgct gtatgacctg gcctcaaaca ccaaaatgta cgccacgaac
    20461 ttcgccctgc aaaagctgat gagcgaaggc aaactgcatc ctgacgatcg gattgcgaaa
    20521 tatattccgg ggtttgccga tagtccaaat gacaccatta aaggcaaaaa caccctgcgg
    20581 atttctgacc tgctgcatca cagcggcggt ttcccggcag atccgcaata cccgaataaa
    20641 gcggtcgcgg gcgcgttata ttaccaggat aaaggccaga cgctggagat gatcaagcgc
    20701 acgccgctag agtatcagcc cggcagcaag catatttaca gtgatgtcga ttatatgctg
    20761 cttggattta tcgtcgagtc ggtcaccggt cagccgcttg accgctatgt tgaagagtcg
    20821 atttatcgcc cgctcggcct gacacatacg gtatttaacc cgctgctgaa aggctttaaa
    20881 ccgcaacaaa ttgccgccac ggaattgaac ggcaataccc gcgatggcgt gatccatttc
    20941 ccgaatatcc gcacctccac tctctggggt caggtgcacg atgaaaaagc cttttattcg
    21001 atgggcggcg tttccgggca cgcaggtttg ttttccaata ccggcgatat tgcggtgtta
    21061 atgcaaacga tgctgaacgg cgggggctat ggtgatgtgc agctgttcaa tgcggaaacg
    21121 gtgaagatgt tcaccaccag ctctaaggaa gatgccactt ttggcctcgg ctggcgcgtg
    21181 aatggtaatg ccaccatgac gccgacgttt ggcacgctgg caagcccgca aacctacggt
    21241 cacactggct ggaccggaac ggtgaccgtt atcgatccgg tgaatcatat gacgattgtg
    21301 atgttaagca acaagccaca ttcgccggtt gccgatccgc aaaagaatcc caatatgttc
    21361 gaaagcggtc agttgccgat tgcaacttat ggttgggtag ttgatcaggt gtatgcggcg
    21421 ttgaagcaaa agtaataaaa aggccagtca gttttgttac tggctggcct ttaatcaatg
    21481 aatcagaaac gcttacagcg ccatcagctt gtccagcgac ggcgcgaagt aatagccgcc
    21541 ggttaccggt ttggtgaaac gcaacatcgc atcacgctta ccatcggtat cgccaaacat
    21601 gctcagcagt tgctgctcaa tgttatgcag acgcgcgcag taggcgcaga agtacagacc
    21661 gtgagtgcca ctggcggtgc cgtacggcag gctctggcga acaatcttca gccctttgcc
    21721 atcttctttc agatcaacgc gggtgaggtg agaggtttcc ggacgttcgt cgccgtcaat
    21781 ctcttcgttg gcctctttgg tgcgcccgat catcatctcc tgatcgtgaa cgctcatccg
    21841 gttgagctgc ttcaggttgt gctcccaacg ctgcacaaac acatagctgc cgccggcatc
    21901 cacgccgtct ttgataaccg ccacttcgcg gcgcgtctct tcacccgccg ggttttctgt
    21961 accgtcaaca aagccgctca ggtcacgctc ttccacccaa cggaagccgt ggatctcttc
    22021 tttcacttca atgcagtcac caaaggcttc catcgccgcc tgagcgacag agaagtttac
    22081 gtcgtgacgc agagaaagaa tgtggatcaa cacatcgaac tgggtcgtcg gcgcaaggcc
    22141 tttaccgtag cccggaaaat ctttcagctc ttctgcccca acgccgccgc tcagagcgca
    22201 ccaggtgttg ttaccaaagg caaccaccgc accaagatgc gcgtccggga atttcgcttc
    22261 aaaagttgcc agtttatcgg caaatgtttt actggccgca cgcagggcgt caacttcccc
    22321 tttcacgttg gcttcgatcc aaatcgccgc gcggcaatgt tctggcaaaa tgccactctg
    22381 aacctgagac attgttcctc ctgaaaataa gaatgccacg caatccgtgg cattgttgag
    22441 cgctatttta cgtgtttttt cgcggcggcg gtttgttcag acgcaaatta acgccgccag
    22501 ataattttac tgactttcca gtttttcagg gtatcgtccg aaggcattaa cccttcagga
    22561 ccacgccatt ccccgctgaa ctggtagcta atatgttgac tgccttcggc tttacattcg
    22621 acggcacgat tatcatcacc atcagctttt tggcaattgc caaatgcttt gctgtaaagg
    22681 tcgctaaacg gtgtaccgat tttaacaccg gtgtcagcag gaatatcgct atccagcacg
    22741 tcaatgcggc tgatcgtgcc ttgctcgccg ttaatcacca tcgccacatt gtcgtctttc
    22801 atcacttcaa aaaagcgcac cacgttgccg ttcgcggttt tcattccgct gcgcaggcga
    22861 taatcgccat caagcgcatc agcaatggct tgttcttgca gtggtgtgga cgccgttaat
    22921 tcacccacgc cctgctcgct cactttggtg gacgacccaa accagttcca cgggttagcg
    22981 gcagaccagt taactgacga catcgtggaa cagccggtca gcatcaacgg catagcgcat
    23041 aacattaaac gcagcgattt catgtcactt cctttggtta ttcaataacg ttgcttggag
    23101 tgcaaattca ccaaaaagtg ccgttaaagg ttgatgacaa acacaaaatt gcctgatgcg
    23161 ctacgcttat caggcctact ttctcctgca atatattgaa ttttcgatgt tttgcaggcc
    23221 ggataaggcg ttctcgccgc atccggcata aacaaagcgc acctctgcgt tattcttttt
    23281 cttcattaaa acaagcgcgt aaacgtcgat tggtcagcag ccagattaac gccacaatat
    23341 ccgccactac cagcgccaga ccgataccgc taacggattc accgttcagc cacagccacg
    23401 gttgccagca aagtaaaacc acctgcgcca acagcaacag aaaatagagt acacgccagg
    23461 tgcgaggaaa cgtagcccgc cgaccgctca acaaaaacgc cagcaccgcc ggaatgccag
    23521 gaataagccc cagccagaaa ttatcgtgat cgggataaaa cagatttagc agcgcagtac
    23581 cctgctcgcg cgacgcaccg gcaatgacaa acagcaccca ggttcgcgcc tgaagcaata
    23641 gcacaagcca gaagagcaag ggtaaacgca ggcgaccgtg cgcatcataa tggacaggat
    23701 gaaactcagt actcttcatc ttcaatcaaa cgcttaccca gactcagcac gtcggcgtgt
    23761 tcatatccca ggcgttcata cattccgagc accatgtcgt tatcttccgg cacattgatc
    23821 tgaattttcg ggcagccacg agcaatcagc tttttctcca gccgattaag caacgcattg
    23881 gcaatcccac gcccacgaaa ctctggatgc acgccaagat aataagcaga cccgcgatgc
    23941 ccgtcataac cgcccatcac cgttccgacc acgtcaccgt ttacctcagc gaccaaaaac
    24001 aaactgacgt catggttcat cttacgctcg atgtccattt ccggatcgtt ccacggacgc
    24061 agcaagtcgc aacgctccca aagggtgatg acctcttcga aatcttcctg gcgaaatacg
    24121 cgtatctcca tggtattcgt tacctttttg cgggttaaaa ggctgattat ggcgtgaacg
    24181 gtcgaattag ccaatatctg acgaaaatcg gttgaaaaag tggcataatg gggagttgtc
    24241 aactattgaa atgaaaagta aaacaattct caacagcaaa ccgtcgtaac ggattacgcg
    24301 atacgatata acatctggaa ctttattatt acaactcagg ccgtatgagc acttttaaac
    24361 cactaaaaac actcacttcg cgccgccagg tgctgaaagc cggtttggct gccctgacgt
    24421 tgtcaggaat gtcgcaagcc atcgccaaag acgaactttt aaaaaccagc aacggacaca
    24481 gcaagccgaa agccaaaaaa tctggcggca aacgtgtcgt tgttctcgat ccaggtcacg
    24541 gcggaattga taccggagcg atcggacgca acggttcgaa agaaaaacat gtggtgctgg
    24601 cgattgctaa aaacgtccgt tccattttgc gtaatcatgg gattgatgcg cgtttaacgc
    24661 gttctggcga tacgtttatc ccactttacg atcgcgttga aatcgcccat aaacatggcg
    24721 cagatctgtt tatgtcaatt catgccgatg gctttaccaa cccgaaagct gccggtgctt
    24781 cggtatttgc cctctctaac cgtggggcaa gtagcgcaat ggcgaaatac ctgtctgaac
    24841 gcgaaaaccg cgccgatgaa gttgccggta aaaaggcgac tgacaaggat cacctattgc
    24901 aacaagtgct gtttgatctg gtgcaaacag ataccattaa aaatagtctg acgctcggct
    24961 cgcatattct gaagaagatt aagccggtgc ataaactgca cagccgcaac accgaacaag
    25021 cggcatttgt ggtgttgaaa tcaccgtcgg ttccttcggt gctggtggaa acctcgttta
    25081 tcaccaaccc ggaagaagaa cggctgttag gcacggcggc gtttcgtcag aaaatcgcca
    25141 cagcgattgc tgaaggcgtg atcagttatt tccactggtt cgacaaccag aaagcacatt
    25201 cgaaaaagcg ataagttatg aaacccgacg cacaccaggt taaacagttt ctgctcaacc
    25261 ttcaggatac gatttgtcag cagctgaccg ccgtcgatgg cgcagaattt gtcgaagata
    25321 gttggcagcg cgaagctggc ggcggcgggc gtagtcgggt gttgcgtaat ggtggtgttt
    25381 tcgaacaggc aggcgtcaac ttttcgcatg tccacggtga ggcgatgcct gcttccgcca
    25441 ccgctcatcg cccggaactt gccgggcgca gtttcgaggc gatgggcgtt tcactggtag
    25501 tgcatccgca taacccgtat gttcccacca gccacgcgaa tgtgcggttt tttattgccg
    25561 aaaaaccggg tgccgatccc gtctggtggt ttggcggtgg cttcgactta accccattct
    25621 atggttttga agaagatgct attcactggc atcgcaccgc ccgtgacctg tgcctgccat
    25681 ttggcgaaga cgtttatccc cgttacaaaa agtggtgcga cgaatacttc tacctcaaac
    25741 atcgcaacga acagcgcggt attggcgggc tgttctttga tgacctgaac acgccagatt
    25801 tcgaccgctg ttttgccttt atgcaggcgg taggcaaagg ctacaccgac gcttatttac
    25861 caattgtcga gcgacggaaa gcgatggcct acggcgagcg cgagcgcaat ttccagttat
    25921 atcgtcgcgg tcgttatgtc gagttcaatc tggtctggga tcgcggcacg ctgtttggcc
    25981 tgcaaactgg cgggcgcacc gagtctatcc tgatgtcaat gccgccactg gtacgctggg
    26041 aatatgatta tcagccaaaa gatggcagcc cagaagcggc gttaagtgag tttattaagg
    26101 tcagggattg ggtgtaactc cctcaccccc actcccgcat ccgctgatgc agcgtcagtg
    26161 acggcttctc ggaaaacagc tgctggtaat ccgtggcaaa ttgccccaga tgccagaatc
    26221 cccactgcat ggcggcgtct tttaccgttg tgctttgcga ccacggactt atcagttcgc
    26281 ggcgtacggc gttcaggcga atgcgtttca gccacgcgtt cgggccaatg cctaaaatag
    26341 cgtgaaacgc gttttgtagc gtgcggcggc tgacatgcag ttgattacac aaatccagca
    26401 ccgtcaccgg ttcggacatg ttttccagca catattcacg ggcgcgggaa agcaatcgac
    26461 ggtaactctg atgactgatg ctttccgccg tcaccattgg ttgcgcttct tccagcatgg
    26521 cccccatcgc cattagcaaa ttatccccca gcacttttcg cactgcaggc tgatggagat
    26581 tttccggatt ctcgcaaaac gtcgccagcg cctgttggac aaagccccac agcgcggctt
    26641 tatgctgctc tttcacttcc agcgccgact ggttacgcaa catatgtaat acccgatccg
    26701 ggttatgcaa aaagttagcc tgccgggtga tgacatcttc agaaagcacc acgcccagga
    26761 tcgtgtaatc atccggcgtg ctcagttcaa attcagtgcc accagggcgg gtggcgattt
    26821 ccgcgcttcc cagacattgc gaaccgataa atccctgctc accgcgcgtc gccggaatgc
    26881 caaaccagaa cgagttcggc cagaccaggc acgactgacg cagcgccaga ccggtgtatt
    26941 cacgaaaaac ctgaatatca tcgagtagaa tttccgtaaa ttcaccatgc aacttgcccg
    27001 gatgcagctg atcgtaaatc tgctgccagg cggtaatcgt taaagcatgt tcatagacat
    27061 ccgttgtctg tcgttgatga acattatcca cttcgacctt cggcgtgagc ttcaggtttt
    27121 cgggtaaggg ttcatgataa agatggtgca aattggctgt acgggtcttt ttcatgatgt
    27181 taatgccggg tgttgtagga cacccggcac ctccgacagg ttaatggggc ttgagacgat
    27241 aacgactact gcgtttacgt aacgtcccgg cagaaaagag ctgttcgagc gcatttctgg
    27301 ctttttccag cggccagcca aagtgggcag ccacttctcc tgccgtcatt ccctgacgta
    27361 ctgatgttaa cagcgccaac aactcgtcag cagattccgc gacgataact ggcgcgtcgg
    27421 gttcccttac gggttgcttc ggctggcggt taaagccagt aaccagccac tgggtgtcat
    27481 cgtctggccg accgatctcc ttgcggctga tgacacgccc ggtacgcatc gcggcagcac
    27541 aacctgcggc cagcgccgca cgacacgccg ccagatcgcc ttccaccacc agcgttaagc
    27601 gaccagggtc aagcacttcg tgactgagca gacgaacgtt agctgctttg agcatggcat
    27661 ccgctgcatc tatcgcggcg accatgccgt ccacttccag caatcccagt gcattgatca
    27721 tcggcaacct ccgttacgca cgctggattg gattacgcgc gatttccagc actgcatcgg
    27781 taaaggcgtt acaggctgct ttacacgcgg cctggctacc ggttaaaaat gctgccgagt
    27841 agttggtttc agacggtggc gggacatagg tcaccagctg tacgtcggca gatttcaacg
    27901 ctgcatcaat gccgtaggtc gcttccagcg gcggtgccac cagatacgcc atcggatcgc
    27961 caagcgtgat cccggcggtt gatgagagat aagaaccggt acgcgaaact acatgtgcca
    28021 ggaaggcggt gttttctgcg tcattggccc actggaaagc tgcgccattt tcaatatgtg
    28081 caaccatcgc atccagaccg gcacgcactt ccgccgggtt tggcccacca agcataatca
    28141 gcacttcacc ggcagtcggt gacgggccgt gtgccgctcc cgcatacagc gagcgaccat
    28201 ataccacttc gaccatcgct tgtttggtcg cttcgtcagc cgcaatgtag gtgacgtcgt
    28261 cagaatcggc agaaatcagc ccgaggctac gaatatgcgg cggcaatttt agttcacgcg
    28321 caaattcagc gttaacggag gcaatcaccc gcatggcggt taccgacggt cgaatcaaat
    28381 ctaaagctgg catgatgcct ccttatcggg tcatgttgat gccggatgct ttctgctcca
    28441 gcatacgttt cgccaaatcc acaatgacgg ctgcggcttc aaccggcggc gtgccgccct
    28501 ggtgaatgtt agagatacag gtgcgatcgg cctcaacggt ggtcgccata cgcggcgagt
    28561 aaacggcgta gcaggagagg ctttctgact gccctaaccc cggacgttcc cccaccagca
    28621 ggatcaccac tttcgcgccg aggatctcac cgatctgatc ttcaatcttc acgcgaccat
    28681 aacgaacaaa gaatggcgtg ccgactttca gcccggcctg tttcaggccc gccatcagcg
    28741 gcggcaggat ctcttcgtag ttgacggtga tggcatcagt tgacaggcca tcagaaataa
    28801 cgacctgtac gtccggatta gcaacacact gtgctttcag cgcttcaaca gcttctgcac
    28861 acaggcggcg gcccatatcc gggcgagtca ggtagagatt tttgtcgcta atttcagagc
    28921 gtacttccag caagccctgc gctttcaccc actcttccgg cacttctttc agtacggtat
    28981 ctttcgaacg agagtgatcg gccaggaaac gcagcagcgc ctgggtacgt ggacgcggac
    29041 cggcacgacc ggtacaaacg cgggccacgg tgctgcggcg cagttctgtt aatacgtctg
    29101 cgcgatgcgg gttttcaaca ccaatccacg cttttgcttc agcggaacct aaatccagcg
    29161 cacagctttc cgaggtcacc ggtgccgcac agttggtggt ggcgcacttt gcttctgacg
    29221 gcgcgggggc cgtttgtccc attgacgcca tcacgctgcg tacaatttct tcaatctgtt
    29281 tttgatccat gatgtgttat ctccgcgtca tcagaagaac agtgacggat cgcccgcccg
    29341 tttggtcagg cgaccgtttg ccataatgcc catgctttcc agccagcgtt caaactccgg
    29401 tgacggacgc aggttgagta actgacgcac agtggcggta tcgtggaatg cggtggtctg
    29461 gtagttgagc atgatgtcat cacccagcgg catccccatg atgtagttgc agcctgcggt
    29521 ggcgagcagg atcatcaggt tttcgttgag gttctggtca gcgtcagcgt ggttggtgta
    29581 gcagcagtca cagcccatag agatgccgct cagcttgccc ataaagtgat cttctaagcc
    29641 agcacggata aagcccttgt cactggtcac ggatggcgca caacattcag caccgcactt
    29701 aacgagtccg gcaggtactc accagactgg atcgagatac aactggccca cgttccaaag
    29761 ggaatcaggg gagtttacaa tcaggcggcc tacctgaagc aacgacgagc aatgatgcaa
    29821 tcgtacgcgg acgccattga ttcaatactg gctggtgacg gcaatccgct ggaaccgtga
    29881 actataaggc cggattcgtc cggcttttct ttcgccttac tggtataaaa ggttaacagg
    29941 ttacacaacc tatcactatt ttatcctgtt atccttttat acagctatac caataaaatc
    30001 aatgattaag ccaagtttgt aataaaaaat ccggggatcg aaaataggat caacgcaaca
    30061 aaaagcccgg aaggccgcta accaatccgg gcgatatggg aaacaagtaa aaaggagaaa
    30121 tcccacatga atattttacc aaccgcctac gccccacggc aaggcgaatc actcggtaaa
    30181 cacagccttc agaaaatcga cgcgatcccg gaagaagtac ggagcgccat tgatgatggc
    30241 ctgaatgata tccgctttaa tggttatcgc tgggaatgca acgccaaaac aggctgggcg
    30301 cgtgtatcac cgatttcatc acctgccgat gcagaacctg aatcaccaga acaaagccag
    30361 gcaaacgccc gcgctgacct cctggcgtta caaggtgagt atttatcagt cacgcaggaa
    30421 actaaaagcg agaagaaatc agctactgat atcgtgaacg agctacgcag taaggccgct
    30481 aaaacttcgc agacagcacc acagcaggaa gcgccggaag aaccggaaca agcggaagcc
    30541 atgcaggata aagagacaca ataccaaacg gaagaaaaag gaatttattg cagctatgtc
    30601 tgtttgggtg cggcacgtaa ggcatttgtc agaagtggtt gcgagaataa agctaaagct
    30661 acagcctgga gcgtattatc cagcattgca tcagcagcaa gaaagcagaa tgatggatct
    30721 tatgtatctg aagtttgcca ggcaaaaata gctgatgaat ggggcatgaa tcgccgcacc
    30781 gtaattcgcc agatgaaaat cctggttgag ttaggactca ttgagccata cggggaatat
    30841 gttattggtc agcacgcaag aaaatacaga atgctttttc aggtcgagaa cgcaagataa
    30901 cgactaaaac gcatcagtgg gtcacctaaa attgtggccc tttttattac ttaaaccatc
    30961 aacagacgtg tgacatttgg gacacaggta cgtgtgacat ttgggataca ggtcagaggg
    31021 tgacgtgtga catttgggat acaggtcagg ccatgacgtg tgacatttgg ggcacacata
    31081 gtatgagatc tgtatattcc ttttttcttt ttaagagaaa gtaagaggcg cgcattttaa
    31141 atgaacacaa acaagcaaca agcccagcgg accttcggaa gacttcgagc aatcgccagc
    31201 tcaaaagacg gtctggcagt gatggttatc atcgacagct ttctgagcta ccgcacagac
    31261 acacagcagc agtaagcaaa ccgaatggaa agcaaagcgc aaaaaactca ctcactgtaa
    31321 agccgatggg ttttatcgcg tatacagacc agacaggaaa cagcagcaaa gcagcgtata
    31381 gccaggtgca gacagtacca tcacacgacg cacagcaggc tgaggtgacc actcaagcaa
    31441 gtttatcccc gccgccgacc accgcaaacc atcctgatag tgccaaattc gaacggagtg
    31501 ctaccttaaa cgcgattaac acacataagc gctacacttg cttacctgaa aacttcacag
    31561 cgtcacagcg cgttttagac acgttaaaat cgatgcgatt tcattaattt ttcagccatc
    31621 cagaaatcgg cttcatatca agccgattca tcagcgcggc gtcctgcatt gcaggttatc
    31681 gaaagaatca ataactttcg ccaatggcga aagttaaaca atcaataaag tccagagttg
    31741 gactattcac ccaaatttgg ggcaatgaaa tcacacagat cctgccaggc tattccatcc
    31801 accacacaga acgctgagat ctgatattac cgccgcacca ttccacgcca gacgaatcaa
    31861 aacaggctta cccttccaag tggatagcta aaaattaagg ctgtcagaat gcgttatatc
    31921 gcgtttaaac ggcatcgaat tttaatgtgc gcaatggtgc cagattgcag gcaaaaaaaa
    31981 tcccgccgga gaaagcgggg aagtgaaatg aatcgttgta gttatagatc atattttgag
    32041 taagggtaac cagcccttga gtaatattat ccctgcatta cgtgagatgc gcaattattt
    32101 tttgctaatt aaaagttttg taaacattac agaatatccc ctttcactat atggtaaaag
    32161 atgtgaaaca gggacaggag gaatataata ttaagtatca acagtaatgt tggcctttaa
    32221 tttcattcag ggcgcggtga gggttttctc catattttcc tcaccgctac tttttgaggt
    32281 gagaacatga atgatagcga attaatgacg gttaacgagg tagcagcttt attcggcgtt
    32341 acccgtcgga caatattccg gtggatgaac aagattaaag gctggcccgt cccggtcagt
    32401 ccgattggct caagaattaa gtttatccga tctgagattt tggaattcta taaaaacaaa
    32461 ggcgctcgcc accaatgaaa acagctactg agattatcaa ataacctatt gatttaaaaa
    32521 gctagctacc gcaatttctg aaggaatagg cagcatgaaa atcaaagcaa acacaattaa
    32581 agaaataaca tcaatcaaag taaaaggagc agatgccatg cattcattag acagttattt
    32641 tcagcgcacg acagccccta aatcagccgc ccaggaacgc cgtgaggaat tccaggaaaa
    32701 agtgatgcgt tctgctgatt atattgcgga caagttcgtg gaaacggttc ggccacttgt
    32761 tgatgaagta gctgataaac ttcagtctga aatgccggaa gacatggaag gcactgcaaa
    32821 ggcgcgactc ctttttgaat taagtcgtcg tttcggcgtg tcaataagca cttttaaata
    32881 atcggaggag gaaagacaat ggacgcattt gatgtactga caaacaaagg cgatgaactg
    32941 gtagcacagc gtgataaaac cgccaacgaa ggggaacgcg cactgttaaa taagcagatt
    33001 aaagcgatcc gtatggccca gtttaaatta atcagcaatg aagttatcga aataattaaa
    33061 ccgcagatcc cacaaatcgt tgctgacgct attaaagccg ccggactgga gaaacgcatt
    33121 gccgggctga aaacgaacgc agagaaaatg gagatgcaca aacagagcgg tgctccgact
    33181 acggatgagt acttctcacc ggatgctgag tttatggcgc aggttgagga acgtctgggt
    33241 agctgtctca ccgaggaaca gcgtcgctat tttgatggag tggatagttc ggcaggtatt
    33301 gacctgaact cgtatttcgg tcgtgaaatt gagcattttg atgccgcccc gtctatgccg
    33361 gagccagaac cagaaatggc cgccgatcct gaagctgagc aacggaaacg cgtattcaat
    33421 gcgataggga ttcggtactg atcggataaa agaaagccgg gaattacccc ggctgactta
    33481 acaatgtgac caatgaacaa atcggacaag acaaacagga taagcagtct cgaaacaatg
    33541 ttcgggaact atattagcac aagtccggag aaaatcatca tgttggcact acgcatcatg
    33601 cagggcatcg ctaagaccct cgcagaacat gtcttagacc tgaaacattc gccactgagc
    33661 aaacaggcca tgaagcgcca aacgcttcgt ctgtgggctg aatattcgct tggaactatc
    33721 aataaaatca tcgacatgaa atcggggccg agcaaccaga gcgcggagga aatggagttc
    33781 attaggcgtt taattctcat tcgcagagac attcatagcc agttacacag cgtgggaatc
    33841 gatattaatg atgggacggg cgattaattt tcggacaagt actatgcgcg cgcgaggagc
    33901 tgaacatgaa gcaagatatt gcagacaggc tggagatact cgaaggccag cgggcagaag
    33961 cgaaacaact acgtaagcag gccagaagag cgcacaggaa caacgaagca gagttactaa
    34021 ccaaatacat cagtttcacc aattactgca tctacgagtg ttacaaggaa gatgcagagg
    34081 actggctgga ttcattgcca gaacagtact gagcagaata acaccacgtc atgttgttct
    34141 gaggtgagca tatcacctct gttcaggtgg ccaaatttag tgtgccactt cagcatcacc
    34201 gttgaaatca caataacgat ttttttgtaa atatgctctc acatatgtag gaatagacca
    34261 ggttggcatt gaaa
//
LOCUS       DAPHBJ010000027        34182 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.027, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000027 DAPHBJ010000000
VERSION     DAPHBJ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.027"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(121..270)
                     /locus_tag="RHZ76_001208"
     CDS             complement(121..270)
                     /locus_tag="RHZ76_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2811878.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
     gene            complement(302..1318)
                     /gene="lplA"
                     /locus_tag="RHZ76_001209"
     CDS             complement(302..1318)
                     /gene="lplA"
                     /locus_tag="RHZ76_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HDV2811879.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            complement(1346..1990)
                     /locus_tag="RHZ76_001210"
     CDS             complement(1346..1990)
                     /locus_tag="RHZ76_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HDV2811880.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            2096..3064
                     /gene="serB"
                     /locus_tag="RHZ76_001211"
     CDS             2096..3064
                     /gene="serB"
                     /locus_tag="RHZ76_001211"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HDV2811881.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            3113..4495
                     /gene="radA"
                     /locus_tag="RHZ76_001212"
     CDS             3113..4495
                     /gene="radA"
                     /locus_tag="RHZ76_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDV2811882.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            4516..5748
                     /gene="nadR"
                     /locus_tag="RHZ76_001213"
     CDS             4516..5748
                     /gene="nadR"
                     /locus_tag="RHZ76_001213"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HDV2811883.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(6055..7722)
                     /gene="ettA"
                     /locus_tag="RHZ76_001214"
     CDS             complement(6055..7722)
                     /gene="ettA"
                     /locus_tag="RHZ76_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HDV2811884.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            7933..9870
                     /gene="sltY"
                     /locus_tag="RHZ76_001215"
     CDS             7933..9870
                     /gene="sltY"
                     /locus_tag="RHZ76_001215"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HDV2811885.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGSEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            9960..10286
                     /gene="trpR"
                     /locus_tag="RHZ76_001216"
     CDS             9960..10286
                     /gene="trpR"
                     /locus_tag="RHZ76_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HDV2811886.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(10371..10892)
                     /gene="yjjX"
                     /locus_tag="RHZ76_001217"
     CDS             complement(10371..10892)
                     /gene="yjjX"
                     /locus_tag="RHZ76_001217"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HDV2811887.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            10944..11591
                     /gene="gpmB"
                     /locus_tag="RHZ76_001218"
     CDS             10944..11591
                     /gene="gpmB"
                     /locus_tag="RHZ76_001218"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HDV2811888.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRYLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            complement(11588..12457)
                     /gene="robA"
                     /locus_tag="RHZ76_001219"
     CDS             complement(11588..12457)
                     /gene="robA"
                     /locus_tag="RHZ76_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HDV2811889.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            12668..13141
                     /gene="creA"
                     /locus_tag="RHZ76_001220"
     CDS             12668..13141
                     /gene="creA"
                     /locus_tag="RHZ76_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HDV2811890.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            13154..13843
                     /gene="creB"
                     /locus_tag="RHZ76_001221"
     CDS             13154..13843
                     /gene="creB"
                     /locus_tag="RHZ76_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HDV2811891.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQAPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            13843..15267
                     /gene="creC"
                     /locus_tag="RHZ76_001222"
     CDS             13843..15267
                     /gene="creC"
                     /locus_tag="RHZ76_001222"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HDV2811892.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRS
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            15325..16677
                     /gene="creD"
                     /locus_tag="RHZ76_001223"
     CDS             15325..16677
                     /gene="creD"
                     /locus_tag="RHZ76_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HDV2811893.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTGAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKANKEVTTDDELRIWK
                     "
     gene            complement(16736..17452)
                     /gene="arcA"
                     /locus_tag="RHZ76_001224"
     CDS             complement(16736..17452)
                     /gene="arcA"
                     /locus_tag="RHZ76_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HDV2811894.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            17548..17688
                     /locus_tag="RHZ76_001225"
     CDS             17548..17688
                     /locus_tag="RHZ76_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2811895.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            18088..18774
                     /locus_tag="RHZ76_001226"
     CDS             18088..18774
                     /locus_tag="RHZ76_001226"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDV2811896.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDTKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            18988..19053
                     /gene="thrL"
                     /locus_tag="RHZ76_001227"
     CDS             18988..19053
                     /gene="thrL"
                     /locus_tag="RHZ76_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HDV2811897.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     misc_feature    18989..19108
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            19135..21597
                     /gene="thrA"
                     /locus_tag="RHZ76_001228"
     CDS             19135..21597
                     /gene="thrA"
                     /locus_tag="RHZ76_001228"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HDV2811898.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLTAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSFIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRSLELADIEIESVLPAEFNAEGDVATFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            21599..22531
                     /gene="thrB"
                     /locus_tag="RHZ76_001229"
     CDS             21599..22531
                     /gene="thrB"
                     /locus_tag="RHZ76_001229"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDV2811899.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            22532..23818
                     /gene="thrC"
                     /locus_tag="RHZ76_001230"
     CDS             22532..23818
                     /gene="thrC"
                     /locus_tag="RHZ76_001230"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDV2811900.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            24032..24328
                     /locus_tag="RHZ76_001231"
     CDS             24032..24328
                     /locus_tag="RHZ76_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDV2811901.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(24481..25257)
                     /gene="yaaA"
                     /locus_tag="RHZ76_001232"
     CDS             complement(24481..25257)
                     /gene="yaaA"
                     /locus_tag="RHZ76_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDV2811902.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDNFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(25327..26757)
                     /locus_tag="RHZ76_001233"
     CDS             complement(25327..26757)
                     /locus_tag="RHZ76_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDV2811903.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SMVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRHPDIGRQLSPDAWDDVSQE"
     gene            27036..27989
                     /gene="tal"
                     /locus_tag="RHZ76_001234"
     CDS             27036..27989
                     /gene="tal"
                     /locus_tag="RHZ76_001234"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDV2811904.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            28104..28691
                     /gene="mog"
                     /locus_tag="RHZ76_001235"
     CDS             28104..28691
                     /gene="mog"
                     /locus_tag="RHZ76_001235"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HDV2811905.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(28726..29292)
                     /gene="satP"
                     /locus_tag="RHZ76_001236"
     CDS             complement(28726..29292)
                     /gene="satP"
                     /locus_tag="RHZ76_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HDV2811906.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(29441..30154)
                     /gene="msyB"
                     /locus_tag="RHZ76_001237"
     CDS             complement(29441..30154)
                     /gene="msyB"
                     /locus_tag="RHZ76_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDV2811907.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(30180..30584)
                     /locus_tag="RHZ76_001238"
     CDS             complement(30180..30584)
                     /locus_tag="RHZ76_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HDV2811908.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            30961..32877
                     /gene="dnaK"
                     /locus_tag="RHZ76_001239"
     CDS             30961..32877
                     /gene="dnaK"
                     /locus_tag="RHZ76_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDV2811909.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            32966..34096
                     /gene="dnaJ"
                     /locus_tag="RHZ76_001240"
     CDS             32966..34096
                     /gene="dnaJ"
                     /locus_tag="RHZ76_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDV2811910.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
BASE COUNT         8322 a         8525 c         9301 g         8034 t
ORIGIN      
        1 atctattcat ttatgttcag ccgtcatcca ggaagggcat atcagcaact ctggtggaca
       61 accataaatt tctgccattt tgcttttgat gtttgagcta aaccgttccg tcttttcgat
      121 ctattttaga gaattaatcg agatacctaa cattttactt acttcagcaa gcgataattt
      181 atgcctgata cgcacggcgg ctagccagga aatacgctgg cttaacatta aattgataat
      241 ctcccgctcc tggttttcca gatcgatcat attcccttcc tttggtattt taatcagcga
      301 gttaccttac cgcccccgct atccacgtcg acaactcccg tagctctttt tcctggtccg
      361 ggaagtcaac caacagcgct tcgcactctt gttgcagcat atccgcgcgg tacaggcagc
      421 cttgcagtcg cccggcgagg gcttccagcg gcgcggggtt gaggctgtcg gtaaacacct
      481 gggcgcgggt gatatggcct ttttcaacgt cgaaatgcag ttccacgccg ccccagctaa
      541 agcgttcatc cagcagatgc gagaatgccg gagcctgacc gaagttccat tcccagctac
      601 tctgacgggc aaaggtttcg gcgaagtttg gcaagtctgg cgttttgtcc ggggagatga
      661 tttccgcttc tacacgctcg ccataatggg cgaaaaaggc cttggttatg gcctcgcaaa
      721 cctgctcatg ggtgatcccc ggcagcagct cggtgaggtt ggtcacgcgg gaacgcactg
      781 aggtaatgcc tttcgccgcc agtttctttt tatccggatt gagatagttt gccaggcggc
      841 taaggtcggc attgagcagc aaagtgccgt ggtggaagcc acgatctttg gtttcgcgat
      901 aggccgagcc tgagactttg cggtcgcctt cgactgtttt caccaccaga tcgttacgtc
      961 cggacgcttc ggcgctgacg ccgagcgcgt tcagcgcatt gagcacaatc gacgtagaga
     1021 tggttttatc gtactccggc ttgccagcca taaaggtaaa gcaggtattg ccgagatcgt
     1081 ggaacaccgc gccgccaccg ctactgcgcc gcgccaggcg gacgttatct tcttccatcc
     1141 gccgggtatt acactctttc cacgggttct gcgcgcgacc aatcactacc gtgtcggcat
     1201 tgcgccagag aaacagaacg cgctgcgtgg cgggcatttg gcgaaaaata cactcttcca
     1261 ccgccaggtt aaaccacggg tcgtaagagt cagagatgag caggcgtaat gtggacataa
     1321 cgatttcctt tcttgtagta atgggtcact cttttttctc gctttcttcc tcttccggca
     1381 ccggtttgct ggcggttaac aggaagggcg attgctgcca gcgggtgcgt ttaccctgta
     1441 gcagcgtgcg ggtcagcact acgccgattg ccagtgagag cagcaacatc aggcgtaaaa
     1501 tgttagtggt gttatccacc tgttgggctt cggtggcgag cgtatgggtg tcgagtgtca
     1561 ggcgcagata gccgagcggt ccgtttttac ccgcaattgg ctcgacaatc tgctggttaa
     1621 aatagccgcc tgctttttta ccgtcgagcg ccagccggtc gcgcacttcg acgctttcgc
     1681 ctgaacgggc gataagatcg ccttgttcgt catacacacc cgcgtcgagg atgcggcttt
     1741 catccgttaa ctgatcgagg atcgcctgaa tgcgtttttc atctggtgag tcggtacgca
     1801 tcagcggtgc aacgttcagc gtcacctgac gcgccagggt gcgggccagt tcttccagct
     1861 gtggattacg ctgtcgctgg tggttttgac taaaccatga cgccccctgc atcagtgcca
     1921 ctaacaaggc aagacagaac aggacaatca ctgcccgatg cagccggaat ttcagttttg
     1981 tgcgagccat cttccaccct ttgaaaattt gagacttaat gttgccagaa gcaatggata
     2041 caaggtagcc tcatgcgtta ttttccctgc ttcgaacgat tttacaggag ccttaatgcc
     2101 taatattacc tggtgcgacc tgcctgaaga tgtctcttta tggcctggtc tgcctctttc
     2161 attaagtggt gatgaagtga tgccactgga ttaccacgca ggtcgtagcg gctggctgct
     2221 gtatggtcgt gggctggata aacagcgtct gacccaatac cagagcaaac tgggtgcggc
     2281 gatggtgatt gttgccgcct ggtgcgtgga agattatcag gtgattcgtc tggcaggttc
     2341 actcaccgca cgggctacgc gcctggccca tgaagcgcaa ctggatgtcg ccccgctggg
     2401 gaaaatcccg cacctgcgca cgccgggttt gctggtgatg gacatggatt ccaccgccat
     2461 ccagattgaa tgtattgatg aaattgccaa actggccgga acgggcgaga tggtggcgga
     2521 agtaaccgaa cgggcgatgc gcggcgaact cgattttacc gccagcctgc gcagccgcgt
     2581 ggcgacgctg aaaggcgctg acgccaatat tctgcaacag gtgcgtgaaa atctgccgct
     2641 gatgccaggc ttaacgcaac tggtgctcaa gctggaaacg ctgggctgga aagtggcgat
     2701 tgcctccggc ggctttactt tctttgctga atatctgcgc gacaagttgc gcctgaccgc
     2761 cgtggtagcc aatgaactgg agatcatgga cggtaaattt accggcaatg tgatcggcga
     2821 catcgtagac gcgcagtaca aagcgaaaac tctgactcgc ctcgcgcagg agtatgaaat
     2881 cccgctggcg cagaccgtgg cgattggcga tggagccaat gacctgccga tgatcaaagc
     2941 ggcagggctg gggattgcct accatgccaa gccaaaagtg aatgaaaagg cggaagtcac
     3001 catccgtcac gctgacctga tgggggtatt ctgcatcctc tcaggcagcc tgaatcagaa
     3061 gtaattgctc gcccgccatc ctgcgggcgg cacagcatta acgaggtaca ccgtggcaaa
     3121 agctccaaaa cgcgcctttg tttgtaatga atgcggggcc gattatccgc gctggcaggg
     3181 gcagtgcagt gcctgtcatg cctggaacac catcaccgag gtgcgtcttg ctgcgtcgcc
     3241 aacggtggcg cgtaacgagc gtctcagcgg ctatgccggt agcgccgggg tggcaaaagt
     3301 ccagaaactc tccgatatca gccttgaaga gctgccgcgt ttttccaccg gatttaaaga
     3361 gttcgaccgc gtactaggcg gcggcgtggt gccaggaagt gccattctga ttggcggtaa
     3421 ccctggtgcg gggaaatcca cgctgttgct gcaaacgctg tgcaaactgg cccagcagat
     3481 gaaaacgctg tatgtcaccg gcgaagagtc gctgcaacag gtggcaatgc gcgctcatcg
     3541 ccttggcctg ccgactgaca atctcaatat gttgtcggaa accagcatcg agcagatctg
     3601 cctgattgcc gaagaagagc aaccgaagct gatggtaatt gactcgatcc aggtgatgca
     3661 tatggcggat gtacagtcat cgcctggcag cgtggcgcag gtgcgtgaaa cggcggctta
     3721 tctgacgcgc ttcgccaaaa cgcgcggtgt ggcgattgtc atggttgggc acgtaaccaa
     3781 agatggctcg ctggctggcc ctaaagtgct ggaacactgt atcgactgtt cggtgcttct
     3841 ggatggtgat gccgactccc gttttcgcac cttgcgcagc cataaaaacc gcttcggcgc
     3901 ggtgaatgag ctgggcgtct tcgcgatgac cgaacagggg ctgcgtgaag tcagcaaccc
     3961 ttcggcaatt ttcttaagtc gcggagatga agtgacctcc ggcagctccg tgatggtggt
     4021 gtgggaagga acgcgtccgt tgctggtgga gattcaggcg ctggtcgatc actcgatgat
     4081 ggcgaatccg cgccgcgtgg cagtggggct ggaacaaaac cgtctggcaa tcctgctggc
     4141 tgtcttgcac cgtcacggtg gtctgcaaat ggccgatcag gatgtatttg tgaacgtggt
     4201 cggcggcgtg aaggtaaccg aaaccagtgc cgatttagct ttactgctgg cgatggtttc
     4261 cagcctgcgt gacagaccgc tgccacagga tctggtggtg tttggtgaag tcgggctggc
     4321 aggggagatc cgcccggtgc ccagcggtca ggaacgaatt tcagaagcgg cgaaacacgg
     4381 ttttcgccgg gcgattgttc cggcggctaa cgtaccgaaa aaagcgccgg aagggatgca
     4441 gatttttggc gttaaaaaac tctccgacgc gcttagcgtg ttcgacgact tataatgaga
     4501 gatacggagg gagatatgtc gtcatttgat tacctgaaaa ctgccatcaa gcaacagggc
     4561 tgcacgctac agcaggtagc tgatgccagc ggtatgacca aagggtattt aagccagtta
     4621 ctgaatgcca aaattaaaag ccccagcgcg caaaagctgg aggcgttgca ccgttttttg
     4681 gggcttgaat ttccccgaca gaagaaaacc attggtgtcg tattcggtaa gttctacccg
     4741 ctgcataccg gacatatcta ccttatccag cgcgcctgta gccaggttga cgagctgcat
     4801 atcattatgg gttttgacga tacccgcgac cgcgcgttgt ttgaagacag cgccatgtcg
     4861 cagcagccca cggtgccgga tcgtctgcgc tggttattac aaacttttaa atatcagaaa
     4921 aatattcgca ttcatgcttt caacgaagag ggcatggagc cgtatccgca cggctgggat
     4981 gtgtggagca acggcatcaa aaagtttatg gctgaaaagg ggattcagcc ggacctgatc
     5041 tacacctcgg aagaagccga tgcgccacag tatatggaac atctggggat cgatacggtg
     5101 ctggtcgatc cgaaacgtac ctttatgagt atcagcggtg cgcagatccg cgaaaacccg
     5161 ttccgctact gggaatatat tcctacggaa gtgaagccgt tctttgtacg taccgtggcg
     5221 atccttggtg gcgagtcgag cggtaaatcc accctggtaa acaaacttgc caatatcttc
     5281 aataccacca gtgcgtggga atatggtcgc gattatgtct tttcacacct cggcggtgat
     5341 gagatcgcat tgcagtattc cgattacgat aaaatcgcgc tgggccacgc acaatacatt
     5401 gattttgcag tgaaatatgc caataaagtg gcgtttattg acactgattt tgtcactacc
     5461 caggcgttct gcaaaaagta cgaagggcgt gagcatccgt tcgtacaggc gttgattgat
     5521 gaataccgtt tcgatctggt gatcctgctg gagaacaaca cgccgtgggt ggcggatggt
     5581 ttacgcagcc tcggcagttc ggtggatcgc aaagagttcc agaacttgct ggtggagatg
     5641 ctggaagaga acaatatcga attcgtgcgg gttgaagagg acgattatga cagccgtttc
     5701 ctgcgctgcg tggagctggt gcgggagatg atgggggagc agagataacc gcgatgaaac
     5761 ggctcaaagg cgaggtataa aataagtttt ttgatgagtt gaaaacctct ctggaagagg
     5821 ctgtcgagat taaacaaggt ttgaaaaaac ctgcacgggt gacccgctac gaaattgcgg
     5881 atactaaggc tgttgtaggc cggaaaaggc gttcacgccg tatccggcat tcggtgctca
     5941 atgcctgatg cgacgcttac cgcgtcttat caggcctgga gattcacaac gtattgaaat
     6001 ttcttggttt gtaggccgga taaggcgttc acgccgcatc cggcatttta cgcattactt
     6061 cgcaatacgc ttgtacttga tacgcttcgg ctccagtgcg tctgcgccca gcgtgcgttt
     6121 cttgtactct tcgtactcag taaagttacc ttcgaagaac tcaactttac cttcatcctg
     6181 gtagtccagg atgtgcgtgg cgatacggtc gaggaaccaa cggtcgtgcg agataaccat
     6241 cgcacagccc gggaactcca gcagggcgtt ttccagcgcg cgcagggttt cgatatccag
     6301 gtcgttggtt ggttcgtcga gcagcagcat gttgccgcca acctgcagca gctttgccag
     6361 atgcagacga ccgcgctcac caccggaaag ttcaccaacg cgtttaccct gatcaacccc
     6421 tttaaagtta aagcggccaa cgtaggcgcg gcttggcatc tcggtgttgc cgatcttcat
     6481 aatatccagc ccgccggaaa cttcttccca aacggttttg ctgttatcca ttgagtcacg
     6541 gaactgatca accgatgcca gtttcaccgt ttcacctaaa gtgatggtgc cgctgtccgg
     6601 ctgttcctga ccagagatca tacggaacag ggtcgattta cccgcgccgt tcggaccgat
     6661 gatcccgacg attgcccctt tcgggatcga gaagctcagg tcatcaatca gcaggcgatc
     6721 accgtaggat ttacgcaggt tgctgacttc cagcacttta tcgcccagac gcggtccagg
     6781 tggaataaac agttcgttgg tttcgttacg tttctgatat tcggtgctgt tcagctcttc
     6841 aaagcgtgcc agacgtgctt tacctttcga ctggcggcct ttagttccct gacgtaccca
     6901 ctccagctct ttctcgatcg acttacgacg cgccgcttct tgtgaagctt cctgcgccag
     6961 gcgctgatct ttctgctcca gccaggagga gtagttgcct tcccacggaa taccttcacc
     7021 gcggtcaagt tcgaggatcc agcctgcaac gttatcgagg aagtaacggt cgtgggtaat
     7081 cgccaccacg gtgccctcga agtcgtgcag gaagcgttcc agccaggcca cggattcggc
     7141 atccaggtgg ttggtcggtt cgtcgagcag cagcatgtct ggtttttcca gcagcaggcg
     7201 gcacaacgct acgcgacgac gctcaccacc ggagaggtta gcgattttcg cgtcccagtc
     7261 cggcagacgt agcgcatccg ccgcacgctc cagctgtacg ttcaggttat gaccgtcgtg
     7321 agcctgaatg atctcttcca gacggccttg ttcagcggcc agcttgtcaa aatcggcatc
     7381 cggatcggcg tacagcgcat acacttcatc caggcgtttc agggcgttaa ccacttcaga
     7441 aaccgcttct tcaatggact cacgcacggt gtgttccggg ttcagctgcg gttcctgcgg
     7501 caggtaacca atcttgatgt ctggctgcgg acgcgcttca ccttcgatgt ctttatcaat
     7561 gcccgccata atgcgcagca gggtagactt acccgcgccg ttcagaccca ggacaccaat
     7621 ttttgcccca gggaagaaac tcagagagat gtttttcaaa atatgacgtt tcggcggaac
     7681 aactttgccg acacgatgca tggtataaac gaattgagcc acgttggact tcgcctctat
     7741 gtttatcgtg atgatgagtt ttcaaaggcg aagtgtagcc tttttccccg cctaatccca
     7801 gttggtcgat caccttcgca gtaaaagtaa aaaagtgtcc gtaacgtggc gtaaacggca
     7861 atgactggtt agcataaatc tattacgcgg catgacgctg cattgatgta tttacactta
     7921 gaggatgcgc ttgtggaaaa agccaaacaa gttacctggc ggctgttggc tgccggtgtc
     7981 tgtctgctga cggtcagcag cgtggcgcga gccgactcac tggatgagca gcgtagccgt
     8041 tacgcgcaaa tcaagcaggc ctgggataat cgacaaatgg atgtggtcga acaaatgatg
     8101 cctggactga aagattatcc actttatccc tacctggaat accgtcagat caccgatgac
     8161 ctgatgaatc aaccggcggt gacggtcact aactttgttc gcgctaaccc cacgcttcct
     8221 cccgctcgca cgctgcaatc tcgtttcgtc aatgaactgg cgcggcgtga agactggcgt
     8281 ggcttgttag cctttagccc ggaaaagccc ggaactaccg aagcgcaatg taattactac
     8341 tatgcaaaat ggaacaccgg gcagagtgaa gaagcctggc aaggggcgaa agagctgtgg
     8401 ctaaccggca agagccagcc taacgcctgt gacaagttat ttagtgtctg gcgtgcgtca
     8461 ggtaaacaag atccgctggc gtatttagag cgtatccgtc tggcgatgaa agcgggtaac
     8521 actggcctgg taacagtgct ggcagggcag atgcctgccg attaccagac tatcgcctcg
     8581 gcaatcattt cactggcgaa caaccctaat acggtactga ccttcgcgcg tacaaccgga
     8641 gcgaccgatt ttacccgtca aatggcggcg gtggcgtttg ccagtgtggc gcggcaggat
     8701 gctgaaaatg cacggctgat gatcccatcg cttgcccagg cgcagcagct taatgaagat
     8761 cagattcagg agctgcgcga tatcgtcgcc tggcgtttga tgggcaacga tgtcaccgac
     8821 gagcaggcga aatggcgcga tgacgccatt atgcgctcgc aatctacttc gcttattgaa
     8881 cgccgtgtac gaatggcgct tggcaccggc gatcgtcgcg gcctgaatac ctggctggcg
     8941 cgtttgccga tggaggcgaa agagaaagat gaatggcgtt actggcaggc ggatttactg
     9001 ctggaacgcg gacgtgaagc tgaagcaaaa gagattttgc atcaactcat gcaacagcgt
     9061 ggtttctacc cgatggttgc tgcacaacgc atcggcgaag agtatgagct gaagattgat
     9121 aaagcgccgc agaatgttga cagcgccctg actcaggggt cggagatggc gcgcgtgcgc
     9181 gagttgatgt actggaatct cgataacacc gcgcgtagcg agtgggccaa tctggtgaag
     9241 agcaagtcaa aaacagagca ggctcaactg gcgcggtatg ctttcaacaa ccaatggtgg
     9301 gatcttagcg ttcaggcaac gatcgccggg aagctgtggg atcatctgga agagcgattc
     9361 ccgctggctt acaacgatct tttcaaacgt tacaccagtg ggaaggagat cccgcaaagc
     9421 tatgcgatgg cgattgcccg tcaggagagc gcctggaatc caaaagtgaa atcaccggta
     9481 ggggccagcg gcctgatgca gattatgcct ggtacagcga cccatacggt gaagatgttc
     9541 tctattcctg gttatagcag ccctgggcaa ttgctggatc cggaaacgaa tatcaacatt
     9601 ggcaccagtt atctgcaata tgtttatcag cagtttggca ataaccgtat tttctcctca
     9661 gcagcttata acgccggacc agggcgggtg cgaacctggc ttggcaacag cgccgggcgt
     9721 atcgacgcag tggcatttgt cgagagtatt ccgttctccg agacgcgtgg ttatgtaaag
     9781 aacgtgctgg cttatgacgc ttactaccgc tatttcatgg gggataaacc gacgttgatg
     9841 agcgccacgg aatggggacg tcgttactga tccgcccgtt tatgatatgc tatcgtactc
     9901 tttagcgagt acaaccgggg gaggcatttt gcttcccccg ctaacaatgg cgacatatta
     9961 tggcccaaca atcaccctat tcagcagcga tggcagaaca gcgtcaccag gagtggttac
    10021 gttttgtcga cctgcttaag aatgcctacc aaaacgatct ccatttaccg ttgttaaacc
    10081 tgatgctgac gccagatgag cgcgaagcgt tggggactcg cgtgcgtatt gtcgaagagc
    10141 tgttgcgcgg cgaaatgagc cagcgtgagt taaaaaatga actcggcgcg ggcatcgcga
    10201 cgattacgcg tggatctaac agcctgaaag ccgcgcccgt tgagctgcgc cagtggctgg
    10261 aagaggtgtt gctgaaaagc gattgatttt gtaggcctga taagacgcgg cagcgtcgca
    10321 tcaggcatcg tgcaccgaat gccggatgcg gcgtgaacgc cttatccgtc ctacaatgcc
    10381 tgatatacgg cattgtgaaa cggactcagc gccaggatca ccgcctggtg atagacgctg
    10441 gcgcgagtga gtttcccggc ggtaaacacg ccgatcgccc cttccttacg accaatctca
    10501 tcaataccgg tataacgcga catcacggga ccaagcgcct cgccttcacg cactttttcc
    10561 agaatcaccg caggcaacgg caaagtagcc gaacgcgcct cgccgcgctg gctggcgttt
    10621 tcaatcacca cccaactgaa agtgctgtca ccatcgatgc cagcttcaat cgccacccaa
    10681 aaatcagcct ctggaagtaa acggcgggca tttgctaccc gatttcgtgc gccagcgcgc
    10741 gtttcctcac tgccaaaggg ttgttccggt acaccgctct cgacggcaac ggatgcaata
    10801 tggcaggatc cttcgccgaa gatctcgtga aatgcctgca gaatggcctg aattttagcg
    10861 ggattggtgg tcgcacagac aacttggtgc ataatcagca ttactcagaa aattaacgtt
    10921 acagcagtat acggaaaaaa agcatgttac aggtatacct agtccgccac ggtgaaacgc
    10981 agtggaacgc cgagcgacgt attcagggcc agtctgacag cccgctgacc gccaaaggtg
    11041 agcaacaggc gatgcaggtg gcaacccgtg ccaaagagct tggcattacg catattatca
    11101 gtagcgattt aggacgcacc cggcgtacgg cggaaatcat cgcccaggcc tgcggctgtg
    11161 acatcatctt tgattctcgc ctgcgtgaat taaacatggg tgtgctggaa aaaagacata
    11221 tcgattctct gaccgaagaa gaagagaact ggcgtcggca gctggtcaat ggcaccgttg
    11281 acgggcgtat tcctgaaggc gagtcaatgc aggagctcag cgatcgcgtc aatgcggcac
    11341 tggaatcttg ccggtactta ccgcagggaa gccgaccgct gctggtgagt catggtattg
    11401 cactgggatg cctggtgagt acgattctcg gattaccagc atgggcagag cgccgcttac
    11461 gtctgcgtaa ctgttctatt tcgcgcgtgg attatcagga aagcctgtgg ctggcgtctg
    11521 gctgggtcgt tgaaaccgca ggggacatct cgcatctgga cgcccctgca ttagatgagc
    11581 tgcagcgtta acggcggata ggaatcagca gttcacagcg cagattaatt gggcgatctc
    11641 ccgctttggc gtcttctgcc gggtagtatc gctcaatatc ctgaccttta cggcgcgtca
    11701 ggttgagcat tggcatgcac gttccgtata ccgtcaggat aaactcctgc acgccggttc
    11761 ccagaccttc ataggtaaac atcacatatt cgccgccctg cagcatcacc ggatgccccg
    11821 tcagtacata gccatctgcc tgatcctggg ctaacgcggt ggtatagaat acctcttgtt
    11881 cgtcgtcttt atcctgactc ggacgcgttt cattcaggcc gtagagcact ggcggaatgg
    11941 tcggcgcgtt gccgagaaaa tcgtgccaga actgataacg catttcatgg cggaaatcag
    12001 agatttgctc cagcgaacag gagtagctct gggtaacacc aatcagcggc gtatcttcca
    12061 gggtgacaaa tttatgctct ggcatagtga attcacccag acgcagcggc gggcgaatac
    12121 caaaggcgct ccattcagga gaacggcggt aaagtgcagg agtctgggca aactgcttct
    12181 tgaatgcgcg ggtaaatgtc tgttgagagt cgaagcggta ttgcagcgcg atgtccagaa
    12241 tcggacgcgc agtcaggcgt agtgcgaccg ccgatttcga caaacgacga gcacgaatat
    12301 acgcgccaat agcatggcca gtgacatctt taaacattct ctgtaagtgc cacttggaat
    12361 aacctgcttt cgccgctaca ttgtcgagcg acaggggctg atccagatga ccttccagcc
    12421 agattaaaag gtcgcgaata atgccggcct gatccataaa atatcctcat cctttcaaca
    12481 acgagcacct gacatcaggt aattggataa tagcattttt tgctgtttta gcattcagtg
    12541 tttttttctt agtagagtat gttttaggtc ttgacggaaa taaaagtatt gagattttgt
    12601 tcttaatcaa tatgttattt accgtgacga actaattgct cgtgtaatag ataaaaatgg
    12661 taacaatatg aaatacaagc atttgatcct gtctttaagc ctgataatgc tggggccatt
    12721 ggctcatgca gaagagattg gttcggtcga caccgtattt aaaatgatcg gcccggatca
    12781 caaaattgtt gtggaagcct ttgacgatcc cgatgtgaaa aatgtcacct gttatgtgag
    12841 ccgggcgaaa acaggtggta ttaaaggggg attgggtctg gcggaagata cctccgatgc
    12901 ggctatttct tgtcagcaag tcgggccgat tgaactgtcg gatcgtatta aaaacggcaa
    12961 agctcagggc gaggtagtat tcaaaaaacg cacgtccctg gtctttaagt cgttacaggt
    13021 cgtgcgcttt tatgacgcca aacgcaacgc gctcgcttat ctggcttact ccgacaaagt
    13081 tgtagaaggt tcgccgaaaa acgcgattag cgcggttcct gtcatgccgt ggcggcaata
    13141 acagaggcga tttatgcaac gggaaacggt ctggttagtg gaagatgagc aagggatagc
    13201 cgacacgctg gtctacatgt tgcagcagga aggttttgcc gtcgaggtct ttgagcgagg
    13261 cttgccggtg ctggataaag ctcgccagca ggctcccgac gtcatgattc tcgatgttgg
    13321 tttgcctgat atcagcggct ttgaattatg ccgccagtta ctggcgctcc atccggcgtt
    13381 acctgtactg ttcctgacgg cccgaagtga agaggtcgat cgcctgcttg ggctggaaat
    13441 tggcgctgac gactatgtgg ctaaaccgtt ttcaccccgc gaagtgtgcg ccagggtgcg
    13501 caccttactg cgtcgggtga agaagttctc gtcgccgtct cccgtcatcc gtattggtca
    13561 ttttgaattg aatgaacccg cggcgcagat cagctggttt gacacgccat taacgctgac
    13621 gcggtatgag tttttattgc tgaagacgtt actcaagtcg ccaggccgcg tctggtcccg
    13681 ccagcaacta atggatagcg tgtgggaaga tgcgcaggac acctacgatc gcaccgttga
    13741 tacccacatt aaaacgttgc gtgccaagct gcgcgccatc aaccccgatc tttcaccgat
    13801 taatactcat cgcggcatgg gatatagcct gaggggcctg taatgcgtat cggcatgcgg
    13861 ttgctgctgg gctatttttt actggtggcg gtggcggcct ggttcgtact ggctattttt
    13921 gtcaaagaag ttaaaccggg cgtgcgaaga gcaacggagg ggacgttaat cgataccgca
    13981 acgttgctgg cggagctggc gcgtcccgat ttgctctctg gggacccaac gcatgggcaa
    14041 ctggcgcagg cgtttaatca gctacaacat cgcccgtttc gcgccaatat cggtggcatt
    14101 aacaaagtgc gcaatgaata tcatgtctat atgaccgatt cgcagggtaa agtattgttc
    14161 gattcggcaa ataaagccgt tgggcaggat tattcgcgct ggaatgacgt ctggctaacg
    14221 ttgcgtggtc agtatggtgc gcgcagcacg ttgcaaaatc ctgccgatcc cgaaagttcg
    14281 gtgatgtatg ttgccgcgcc gattatggac ggctcgcggc ttattggcgt tttgagcgta
    14341 ggcaaaccga acgcggcgat ggctccggtc attaagcgta gcgagcggcg aattttatgg
    14401 gccagcgcca ttttgctggg gattgcactg gtgattggcg caggcatggt ttggtggatc
    14461 aaccgctcca ttgcccggct cactcgctat gccgattccg tcactgacaa taagcccgtt
    14521 cctcttcctg aactcggcag tagcgagttg cgtaagcttg cgcaggcgct ggaaagtatg
    14581 cgcgtgaagc tggaagggaa aaactatatt gagcagtatg tttatgcgtt aacccatgag
    14641 ctaaaaagcc cactggcggc gattcgtagc gcggcggaaa ttttacgcga aggtccgcca
    14701 ccggaagtgg tggctcgttt taccgacaac attctgacgc aaaatgcgcg tatgcaggca
    14761 ttggtagaaa cgttactacg ccaggcaaga ctggagaatc gtcaggaagt cgttctgact
    14821 gttgttgatg tggcggcatt atttcgccgc gtcagcgaag cgcgcaccgt gcagttggca
    14881 gaaaaaaaca tcactctaca tgttatgccc actgaggtta atgttgctgc tgaaccggcg
    14941 ttactggagc aggcgctggg gaatttactg gataacgcca tcgattttac ccccgagagc
    15001 ggtcgcataa cgctaagcgc cgaagtggat caggaacacg tcgcccttaa ggtgctggat
    15061 accggtagtg gtattcctga ctacgcgctg tcacgtattt ttgaacgctt ttactctttg
    15121 ccgcgtgcaa atgggcaaaa aagcagcggt ctggggttgg cgttcgtcag tgaggtcgcc
    15181 cgtttgttta acggcgaagt cacgctgcgc aacgtgcagg aaggtggcgt gctggcctcg
    15241 cttcgacttc accgtcactt cacatagctt caaattcttc ccacatagcc ttcgtatcct
    15301 gctgccattg caaaggagaa gactatgttg aaatcccccc tgttctggaa aatgaccacc
    15361 ctgtttggtg ccgtattgct gttgttgatt ccgataatgc tgattcggca ggtgattgtc
    15421 gaacgtgccg attaccgtag cgatgtggaa gatgcgattc gccaaagtac cagcgggccg
    15481 caaaaactcg ttgggccgct tatcgctatt cctgtgaccg agctttatac ggtacaggaa
    15541 gaggataaaa ccgtggagcg gaaacgaagt tttatccatt tttggttacc tgagtcattg
    15601 atggttgatg gcaatcagaa cgtggaagaa cgcaagatag ggatttatac cggtcaggtc
    15661 tggcacagtg atttaacgct aaaagccgat ttcgatgttt cgcgtcttag cgaactcgac
    15721 gcgccaaata tcacctttgg caagccattt attgtgatta gcgtcgggga tgcgcgtggt
    15781 attggtgtgg tgaaagcgcc tgaagttaac ggaacggcgc tgaccattga acccggcacc
    15841 gggttagagc aaggcgggca gggcgtgcat atccctttac ccgaagggga ctggcggaag
    15901 cagaacctgc agctgaatat ggcactgaat ttaagcggta ccggcgatct ttctgtggtg
    15961 cctgccgggc gtaatagcga aatgacctta accagcaact ggccgcatcc cagtttttta
    16021 ggtgattttc taccagccaa acgggaagtt agcgagtcag gttttcaggc gcaatggcaa
    16081 agcagctggt ttgctaataa tctcggtgag cgttttgctt caggcaatga taccggctgg
    16141 gaaaacttcc ccgcgtttag cgtcgcagtg acgacgccag ccgatcaata ccaattaact
    16201 gaccgggcga ctaagtacgc tattctgctg attgcgctga cttttatggc gttctttgtt
    16261 tttgaaacgc tcaccgcgca acgtttacac ccaatgcaat atttactggt ggggctttca
    16321 ttagtgatgt tttatttgct cttgctggcg ctttctgaac ataccggttt taccggggca
    16381 tggataatcg ccagtctgat tggggcgtta atgaacggta tttatttgca ggcggtattg
    16441 aaaggctggc gcaacagcat gttgtttacc ctcgcgctgt tgttgctgga tggtgtgatg
    16501 tggggactac tcaactctgc cgatagcgcg ctgttgttgg gaaccagtgt gctggtggtg
    16561 gcactggccg gaatgatgtt tgtgacccgt aatatcgact ggtatgcgtt ttcactgccg
    16621 aaaatgaaag ccaataaaga agttacaacg gacgatgaat tacgtatctg gaaataaggt
    16681 tgaaaaaaaa aacggcgcta aaaagcgcca ttttttttga cggtggtaac gccgattaat
    16741 cttccagatc accgcagaag cgataacctt caccgtggat ggtggcgatg atttccggcg
    16801 tatccggcgt agattcgaaa tgtttacgaa tacggcggat cgtcacgtct acagtacggt
    16861 cgtgtggttt cagctcacgg ccggtcattt tcttcagcag ttcagcacgg gactgaattt
    16921 tgcctgggtt ttcacagaag tgaagcatgg cgcggaactc gctgcgcggc agcttgtact
    16981 gctcgccatc agggccgatc aacgaacggc tgttgatgtc cagttcccaa ccattgaact
    17041 tgtagctttc aacgctacga cgttcttcgc tgacagtacc cagattcatg gtacgggaca
    17101 gcaggttgcg tgcacgaata gtcagttcac gcgggttgaa cggtttggtg atgtagtcat
    17161 ctgcaccgat ttcgaggccg agaattttat cgacttcgtt gtcacggcca gtcaggaaca
    17221 tcaacgcaac attcgcctgc tcgcgcagtt cacgcgctaa cagaagaccg ttcttacccg
    17281 gcagattgat atccatgatc accaggttga tgtcatattc agagaggatc tgatgcattt
    17341 ccgcgccatc tgtcgcttcg aaaacatcat agccttccgc ttcgaaaata cttttcaacg
    17401 tgttgcgtgt taccaactcg tcttcaacga taagaatgtg cggggtctgc atgtttgcta
    17461 cctaaattgc caactaaatc gaaacaggaa gtacaaaagt ccctgacctg cctgatgcat
    17521 gctgcaaatt aacatgatcg gcgtaacatg actaaagtac gtaattgcgt tcttgatgca
    17581 ctttccatca acgtcaacaa catcattagc ttggtcgtgg gtactttccc tcaggacccg
    17641 acagtgtcaa aaacggctgt catcctaacc attttaacag caacataaca ggctaagagg
    17701 ggccggacac ccaataaaac tacgcttcgt tgacatatat caagttcaat tgtagcacgt
    17761 taacagtttg atgaaatcat cgtatctaaa tgctagcttt cgtcacatta ttttaataat
    17821 ccaactagtt gcatcataca actaataaac gtggtgaatc caattgtcga gatttatttt
    17881 ttataaaatt atcctaagta aacagaagga tatgtagcat tttttaacaa ttcaaccgtt
    17941 agtacagtca ggaaatagtt tagccttttt taagctaagt aaagggcttt ttctgcgact
    18001 tacgttaagg atttgtaaat tctcaccgcg taataagttg acagtgatca ccctgttcgc
    18061 ggtcatttgg tcaagaagag tggcaatatg cgtataacga ttattctggt cgcacccgcc
    18121 agagcagaaa atattggggc agcggcgcgg gcaatgaaaa cgatggggtt tagcgaactg
    18181 cggattgtcg atagtcaggc acatctggag ccagccactc gctgggtcgc acatggatct
    18241 ggtgatatta ttgataatat taaagttttc ccgacactgg ttgaatcgtt acacgatgtc
    18301 gatttcactg tcgccaccac tgcgcgcagt cgggcgaaat atcattacta cgcaacgcca
    18361 gtagaactgg tgccgctgtt agaggaaaaa tcttcatgga tgagccatgc cgcgctggtg
    18421 tttggtcgcg aagattccgg gctgactaac gaagagttag cgttggctga cgttcttact
    18481 ggtgtgccga tggtggcgga ttatccttcg ctcaatctgg ggcaggcagt gatggtctat
    18541 tgctatcaat tagcaacatt aatacaacaa ccgacgaaaa gtgacacaac ggcagaccaa
    18601 catcaactgc aagctttacg cgaacgagtc atggcattgc tgacgactct ggcagtggca
    18661 gatgacacaa aactggtcga ctggttacaa caacgtctgg ggcttttaga gcagcgagac
    18721 acggcaatgt tgcaccgttt gctgcatgat attgaaaaaa atatcaccaa ataaaaaacg
    18781 ccttagtaag tatttttcag cttttcattc tgactgcaat gggcaatatg tctctgtgtg
    18841 gattaaaaaa agagtctctg acagcagctt ctgaactggt tacctgccgt gagtaaatta
    18901 aaattttatt gacttaggtc actaaatact ttaaccaata taggcatagc gcacagacag
    18961 ataaaaatta cagagtacac aacatccatg aaacgcatta gcaccaccat taccaccacc
    19021 atcaccatta ccacaggtaa cggtgcgggc tgacgcgtac aggaaacaca gaaaaaagcc
    19081 cgcacctgac agtgcgggct ttttttttcg accaaaggta acgaggtaac aaccatgcga
    19141 gtgttgaagt tcggcggtac atcagtggca aatgcagaac gttttctgcg ggttgccgat
    19201 attctggaaa gcaatgccag gcaggggcag gtggccaccg tcctctctgc ccccgccaaa
    19261 atcaccaacc acctggtggc gatgattgaa aaaaccatta gcggccagga tgctttaccc
    19321 aatatcagcg atgccgaacg tatttttgcc gaacttctga caggactcac cgccgcccag
    19381 ccgggattcc cgctggcgca actgaaaact ttcgtcgacc aggaatttgc ccaaataaaa
    19441 catgtcctgc atggcattag tttgttgggg cagtgcccgg atagcatcaa cgctgcgctg
    19501 atttgccgtg gcgagaaaat gtcgatcgcc attatggccg gcgtgttaga agcgcgtggt
    19561 cacaacgtta ccgttatcga tccggtcgaa aaactgctgg cagtggggca ttacctcgaa
    19621 tctaccgtcg atattgctga gtccacccgc cgtattgcgg caagtcgtat tccggctgat
    19681 cacatggtgc tgatggcagg tttcaccgcc ggtaatgaaa aaggcgaact ggtggtgctt
    19741 ggacgcaacg gttccgacta ctccgcggcg gtgctggctg cctgtttacg cgccgattgt
    19801 tgcgagattt ggacggacgt tgacggggtc tatacctgcg acccgcgtca ggtgcccgat
    19861 gcgaggttgt tgaagtcgat gtcctaccag gaagcgatgg agctttccta cttcggcgct
    19921 aaagttcttc acccccgcac cattaccccc atcgcccagt tccagatccc ttgcctgatt
    19981 aaaaataccg gaaatcctca agcaccaggt acgctcattg gtgccagccg tgatgaagac
    20041 gaattaccgg tcaagggcat ttccaatctg aataacatgg caatgttcag cgtttccggc
    20101 ccggggatga aaggaatggt cggcatggcg gcgcgcgtgt ttgcagcgat gtcacgcgcc
    20161 cgtatttccg tggtgctgat tacgcaatca tcttccgaat acagtatcag tttctgcgtt
    20221 ccgcaaagcg actgtgtgcg agctgaacgg gcaatgcagg aagagttcta cctggaactg
    20281 aaagaaggct tactggagcc gctggcggtg acggaacggc tggccattat ctcggtggta
    20341 ggtgatggta tgcgcacctt gcgtgggatc tcggcgaaat tctttgccgc gctggcccgc
    20401 gccaatatca acattgtcgc cattgctcag ggatcttctg aacgctcaat ctctgtcgtg
    20461 gtaaataacg atgatgcgac cactggcgtg cgcgttactc atcagatgct gttcaatacc
    20521 gatcaggtta tcgaagtatt tgtgattggc gtcggtggcg ttggcggtgc gctgctggag
    20581 caactgaagc gtcaacaaag ctggctgaag aataaacata tcgacttacg tgtctgcggc
    20641 gttgccaact cgaaggctct gcttaccaat gtgcatggcc taaacctgga aaactggcag
    20701 gaagaactgg cgcaagccaa agagccgttt aatctcgggc gcttaattcg cctcgtgaaa
    20761 gaatatcatc tgctaaaccc ggtcattgtt gactgcacct ccagccaggc agtggcggat
    20821 caatatgccg acttcctgcg cgaaggtttc catgtggtga ccccgaacaa aaaggccaac
    20881 acctcgtcga tggattacta ccatcagctg cgttacgcgg cggaaaaatc acggcgtaaa
    20941 ttcctttatg acaccaacgt cggtgcgggg ttgccggtca ttgagaactt gcaaaacctg
    21001 ctcaacgcgg gtgatgaatt gatgaaattc tctggcattc tttctggttc gctttctttc
    21061 atcttcggta agttagacga agggatgagc ttctccgagg ccaccacgct ggcaagagaa
    21121 atgggctata ccgaaccgga cccgcgagat gatctttctg gtatggatgt ggcgcgcaag
    21181 ctgttgattc tcgcccgtga aacaggacga tcactggaac tggcggatat cgaaatcgaa
    21241 tctgtgctgc ccgcagagtt taacgcggaa ggcgatgttg cgacttttat ggcgaatctg
    21301 tcacagctcg acgatctctt tgccgcgcgc gtggcgaagg cccgtgatga aggaaaagtt
    21361 ttgcgctatg ttggcaatat tgatgaagat ggcgtctgcc gcgtgaagat tgctgaagtg
    21421 gatggcaacg atccgctgtt caaagtgaaa aacggcgaaa acgcattggc cttctatagc
    21481 cactattatc agccactgcc gctggtcctg cgcggatacg gtgcgggtaa tgacgttaca
    21541 gctgccggtg tctttgccga tctgctacgc accctctcat ggaagttagg agtctaaaat
    21601 ggttaaggtt tatgccccgg cttccagtgc caatatgagc gtcgggtttg atgtgctcgg
    21661 ggcggcggtg acacccgttg atggtgcatt gctcggagat gtagtcacgg ttgaggcggc
    21721 agagacattc agtctcaaca acctcggacg ctttgccgat aagctgccgt cagaaccacg
    21781 ggaaaatatc gtttatcagt gctgggagcg tttttgccag gagcttggca agcaaattcc
    21841 agtggcgatg actctggaaa agaatatgcc aatcggttcg ggcttaggct ccagcgcctg
    21901 ttcggtggtc gcggcgctga tggcgatgaa tgaacactgt ggcaagccgc ttaatgacac
    21961 tcgtttgctg gctttgatgg gcgagctgga aggacgaatc tccggcagca ttcattacga
    22021 caacgtggca ccgtgttttc ttggtggtat gcagttgatg atcgaagaaa acgacatcat
    22081 cagccagcaa gtgccagggt ttgatgagtg gctgtgggtg ctggcgtatc cggggattaa
    22141 agtctcgacg gcagaagcca gggctatttt accggcgcag tatcgccgcc aggattgcat
    22201 tgcgcacggg cgacatctgg ctggcttcat tcacgcctgc tattcccgtc agcctgagct
    22261 tgccgcgaag ctgatgaaag atgttatcgc tgaaccctac cgtgaacggt tactgcctgg
    22321 cttccggcag gcgcggcagg cggttgcgga aatcggcgcg gtagcgagcg gtatctccgg
    22381 ctccggcccg accttgttcg ctctgtgtga caagccggat accgcccagc gcgttgccga
    22441 ctggttgggt aagaactacc tgcaaaatca ggaaggtttt gttcatattt gccggctgga
    22501 tacggcgggc gcacgagtac tggaaaacta aatgaaactc tacaatctga aagatcacaa
    22561 tgagcaggtc agctttgcgc aagccgtaac ccaggggttg ggcaaaaatc aggggctgtt
    22621 ttttccgcac gacctgccgg aattcagcct gactgaaatt gatgagatgc tgaagctgga
    22681 ttttgtcacc cgcagtgcga agatcctctc ggcgtttatt ggtgatgaaa tcccgcagga
    22741 aatcctggaa gagcgcgtgc gcgcggcgtt tgccttcccg gctccggtcg ccaatgttga
    22801 aagcgatgtc ggttgtctgg aattgttcca cgggccaacg ctggcattta aagatttcgg
    22861 cggtcgcttt atggcacaaa tgctgaccca tattgcgggc gataagccag tgaccattct
    22921 gaccgcgacc tccggtgata ccggagcggc agtggctcat gctttctacg gtttaccgaa
    22981 tgtgaaagtg gttatcctct atccacgagg caaaatcagt ccactgcaag aaaaactgtt
    23041 ctgtacattg ggcggcaata tcgaaactgt tgccatcgac ggcgatttcg atgcctgtca
    23101 ggcgctggtg aagcaggcgt ttgatgatga agagctgaaa gtggcgctgg ggttaaactc
    23161 agctaactcg attaacatca gccgtttgct ggcgcagatt tgctactact ttgaagcagt
    23221 tgcgcagctg ccgcaggaag cgcgcaacca gctggttgtc tcggtgccaa gcggaaactt
    23281 cggcgatttg acggcgggtc tgctggcgaa gtcactcggt ctgccggtga aacgttttat
    23341 tgctgcgacc aacgtgaacg ataccgtgcc acgtttcctg cacgacggtc agtggtcacc
    23401 caaagcgact caggcgacgt tatccaacgc gatggacgtg agtcagccga acaactggcc
    23461 gcgtgtggaa gagttgttcc gccgcaaaat ctggcaactg aaagagctgg gttatgcagc
    23521 cgtggatgat gaaaccacgc aacagacaat gcgtgagtta aaagaactgg gctacacttc
    23581 ggagccgcac gctgccgtag cgtatcgtgc gctgcgtgac cagttgaatc caggcgaata
    23641 tggcttgttc ctcggcaccg cgcatccggc gaaatttaaa gagagcgtgg aagcgattct
    23701 cggtgaaacg ttggatctgc caaaagagct ggcagaacgt gctgatttac ccttgctttc
    23761 acataatctg cccgccgatt ttgctgcgtt gcgtaaattg atgatgaatc atcagtaaaa
    23821 tctattcatt atctcaatca ggccgggttt gcttttatgc agcccggctt ttttatgaag
    23881 aaattatgga gaaaaacgac agggaaaaag gagaaattct caataaatgc ggtaacttag
    23941 agattaggat tgcggagaat aacaaccgcc gttctcatcg cgtaatctcc ggatatcgac
    24001 ccataacggg caatgataaa aggagtaacc tgtgaaaaag atgcaatcta tcgtactcgc
    24061 actttccctg gttctggtcg ctcccatggc agcacaggct gcggaaatta cgttagtccc
    24121 gtcagtaaaa ttacagatag gcgatcgtga taatcgtggc tattactggg atggaggtca
    24181 ctggcgcgac cacggctggt ggaaacaaca ttatgaatgg cgaggcaatc gctggcaccc
    24241 acacggaccg ccgccaccgc cgcgtcacca taagaaagct catcatgatc atcacggcgg
    24301 tcatggtcca ggcaaacatc accgctaaat gacaaatgcc gggtaacaat ccggcattca
    24361 gcgcctgatg cgacgctggc gcgtcttatc aggcctacgt gaattctgca atatattgaa
    24421 tctgcatgct tttgtaggcc ggataaggcg ttcacgccgc atccggcatt gactgcaaac
    24481 ttaacgctgc tcgtagcgtt taaacaccag ttctccattg ctggaggaag cttcatcaaa
    24541 gaagtaacct tcgctattaa aaccagtcag ttgctctggt ttggtcagcc gattttcaat
    24601 aataaaacga ctcatcagac cgcgtgcttt cttagcgtag aagctgatga tcttaaattt
    24661 gccgttcttc tcatcgagga acaccggctt gataatctcg gcattcaatt tcttcggctt
    24721 caccgattta aaatactcat ctgacgccag attaatcacc acattatcgc cttgtgctgc
    24781 gagcgcctcg ttcagcttgt tggtgatgat atctccccag aattgataca gatctttccc
    24841 tcgggcattc tcaagacgga tccccatttc cagacgataa ggctgcatta aatcgagcgg
    24901 gcgcagtacg ccatacaagc cggaaagcat tcgcaaatgc tgttgggcaa aatcgaaatt
    24961 gtcttcgctg aaagtttcgg cctgcaagcc ggtgtagaca tcacctttaa acgccagaat
    25021 cgcctggcgg gcattctccg gcgtgaaatc tggctgccag tcatgaaagc gagcggcgtt
    25081 gatacccgcc agtttgtcgc tgatgcgcat cagcgtgcta atctgcggag gcgtcagttt
    25141 ccgcgcttca tggatcaact gctgggaatt gtctaacagc tccggcagcg tatagcgcgt
    25201 ggtggtcaac gggctttggt aatcaagcgt tttcgcaggt gaaataagaa tcagcatatc
    25261 cagtccttgc aggaaattta tgccgacttt agcaaaaaaa gagaatgagt tgatcgatag
    25321 ttgtgattac tcctgcgaaa catcatccca cgcgtccgga gaaagctggc ggccgatatc
    25381 cggatgacgc aatggatcaa acaccgggcg cacgccgagt ttacgctggc gtagataatc
    25441 actggcaatg gtatgaacca caggcgagag cagtaaaatg gcggtcaaat tggtaatagc
    25501 catgcaggcc attatgatat ctgccagttg ccacatcagc ggaagactta gcaaggtgcc
    25561 gccgatgacc gttgcgaagg tgcagatccg caaacaccag atcgctttag ggttgttcag
    25621 gcgtaaaaag aagagattgt tttcggcata aatgtagttg gcaacgatgg agctgaaggc
    25681 aaacagaata accacgaggg taacaaactc agcaccccag gaacccatta gcacccgcat
    25741 cgccttctgg ataagctgaa taccttccag cggcatgtag gttgtgccgt tacccgccag
    25801 taatatcagc atggcgcttg ccgtacagat gaccagggtg tcgataaaaa tgccaatcat
    25861 ctggacaatt ccttgcgctg ccggatgcgg aggccaggac gccgctgccg cagccgcgtt
    25921 tggcgtcgaa cccattcccg cctcattgga aaacatactg cgctgaaaac cgttagtaat
    25981 cgcctggctt aaggtatatc ccgccgcgcc gcctgccgct tcctgccagc caaaagcact
    26041 ctcaaaaata gaccaaatga cgtggggaag ttgcccgata tttattacgc aaatcaccat
    26101 gctggtcagc acccagatta tcgccatcaa cgggacaaaa ccctgcatga gccgggcgac
    26161 gccatgaaga ccgcgagtga ttgccagcag agcaaagaca gcgagaataa tgcctgtcac
    26221 cagcggggga aaatcaaaag aaaaactcag ggctcgggca actgcgtttg cctgaactcc
    26281 gctgaaaatt atgccgtagg cgatgagcaa aaagacggcg aacagaacgc ccatccagcg
    26341 catccccagc ccgcgcgcca tataccatgc cggtccgcca cgaaactgcc cattgacgtc
    26401 acgttcttta taaagttgtg caagggaaca ttcggcaaac gaggtcgcca tgccgataaa
    26461 cgcggcaacc cacatccaaa agacggctcc aggtccaccg gcggtaattg ccagcgcaac
    26521 gccggccagg ttgccgctac ccacgcgcgc cgcaagactg gtacacaatg actgaaatga
    26581 ggttaaaccg cctggctgtg gatgaatgct atttttaaga cttttgccaa actggcggat
    26641 gtagcgaaac tgcacaaatc cggtgcgaaa agtgaaccaa caacctgcgc cgaagagcag
    26701 gtaaatcatt accgatcccc aaaggacgct gttaataaag gagaaaaaat ctggcatgca
    26761 tatccctctt attgccggtc gcgatgactt tcctgtgtaa acgttaccaa ttgtttaaga
    26821 agtatatacg ctacgaggta cttgataact tctgcgtagc atacatgagg ttttgtataa
    26881 aaatggcggg cgatatcaac gcagtgtcag aaatccgaaa cagtctcgcc tggcgataac
    26941 cgtcttgtcg gcggttgcgc tgacgttgcg tcgtgatatc atcagggcag accggttaca
    27001 tccccctaac aagctgttta aagagaaata ctatcatgac ggacaaattg acctcccttc
    27061 gtcagtacac caccgtagtg gccgacactg gggacatcgc ggcaatgaag ctgtatcaac
    27121 cgcaggatgc cacaaccaac ccttctctca ttcttaacgc agcgcagatt ccggaatacc
    27181 gtaagttgat tgatgatgct gtcgcctggg cgaaacagca gagcaacgat cgcgcgcagc
    27241 agatcgtgga cgcgaccgac aaactggcag taaatattgg tctggaaatc ctgaaactgg
    27301 ttccgggccg tatctcaact gaagttgatg cgcgtctttc ctatgacacc gaagcgtcaa
    27361 ttgcgaaagc aaaacgcctg atcaaactct acaacgatgc tggtattagc aacgatcgta
    27421 ttctgatcaa actggcttct acctggcagg gtatccgtgc tgcagaacag ctggaaaaag
    27481 aaggcatcaa ctgtaacctg accctgctgt tctccttcgc tcaggctcgt gcttgtgcgg
    27541 aagcaggcgt gttcctgatc tcgccatttg ttggccgtat tcttgactgg tacaaagcga
    27601 atactgataa gaaagagtac gctccggcag aagatccggg cgtggtttct gtatctgaaa
    27661 tctaccagta ctacaaagag cacggttatg aaaccgtggt tatgggcgca agcttccgta
    27721 acatcggcga aattctggaa ctggcaggct gcgaccgtct taccatcgca cctacactgc
    27781 tgaaagagct ggctgagagc gaaggggcta tcgaacgtaa actgtcttac accggcgaag
    27841 tgaaagcgcg tccggcgcgt atcactgagt ccgagttcct gtggcagcac aaccaggatc
    27901 caatggcagt agataaactg gcggaaggta tccgtaagtt tgctattgac caggaaaaac
    27961 tggaaaaaat gatcggcgat ctgctgtaat cattcttagc gtgaccggga agtcggtcac
    28021 gctacctctt ctgaagcctg tctgtcactc ccttcgcagt gtatcattct gtttaacgag
    28081 actgtttaaa cggaaaaatc ttgatgaata ctttacgtat tggcttagtt tccatctctg
    28141 atcgcgcatc cagcggcgtt tatcaggata aaggcatccc tgcgctggaa gaatggctga
    28201 catcggcgct aaccacgccg tttgaactgg aaacccgctt aatccccgat gagcaggcga
    28261 tcatcgagca aacgttgtgt gagctagtgg atgaaatgag ttgccatctg gtgctcacca
    28321 cgggcggaac tggcccggcg cgtcgtgacg taacgcccga tgcgacgctg gcagtagcgg
    28381 accgcgagat gcctggcttt ggtgaacaga tgcgccagat aagcctgcat tttgtaccaa
    28441 ctgcgatcct ttcgcgtcag gttggtgtga ttcgcaaaca ggcgctgatc cttaacttac
    28501 ccggtcagcc gaagtctatt aaagagacgc tggaaggtgt gaaggacgct gcgggtaacg
    28561 ttgtggtaca cggtattttt gccagcgtac cgtactgcat tcagttgctg gaagggccat
    28621 acgttgaaac ggcaccagaa gtggttgctg cattcagacc gaagagtgca agacgcgacg
    28681 ttagcgaata aaaaaatccc cccgagcggg gggatctcaa aacaattagt gggattcacc
    28741 aatcggcaga acggtgcgac caaactgctc gttcagtact tcacccatcg ccagatagat
    28801 tgcgctggca ccgcagatca gaccaatcca gccggcaaag tggatgattg cggcgttacc
    28861 ggcaatgtta ccgatcgcca gcagggcaaa cagcacggtc aggctaaaga aaacgaattg
    28921 cagaacgcgt gcgcctttca gcgtgccgaa gaacataaac agcgtaaata cgccccacag
    28981 acccaggtag acaccaagga actgtgcatt tggcgcatcg gtcagaccca gtttcggcat
    29041 cagcagaatc gcaaccagcg tcagccagaa agaaccgtaa gaggtgaatg cggttaaacc
    29101 gaaagtgttg ccttttttgt actccagcag accggcaaaa atttgcgcga tgccgccgta
    29161 gaaaatgccc atggcaagaa taataccgtc cagagcgaaa taacccacgt tgtgcaggtt
    29221 aagcagaatg gtggtcatgc cgaagcccat caggcccagc ggtgccggat tagccaactt
    29281 agtgttgccc ataattcctc aaaaatcatc atcgaatgaa tggtgaaata atttccctga
    29341 ataactgtag tgttttcagg gcgcggcata ataatcagcc agtggggcag tgtctacgat
    29401 cttttgaggg gaaaatgaaa attttccccg gtttacggtg tcagacctga gtggcgctaa
    29461 ccatccggcg caggcaggcg atttgcagta cggctggaat tgtcacgcga taggcactgc
    29521 cgctgaccgc tttaacccca tttaatgctg cgcccacagg gcctcccaga cccgcgccgc
    29581 gcagcaaacc atgcccaagc acgctcattg ctgcgtgggt gcgtaaaatg cgggtcagtt
    29641 ggctggaaag caaatgcgac acgccttttg ccaataattt gtctttcatc agcagcggca
    29701 gcagctcttc cagctcattc accctggcat cgaccgcgtg cagaaactcc tgcttatgtt
    29761 cctcgtccat attcttccag gtgttacgca gaaattgttc cagtaactgt tgctcaatct
    29821 caaacgtaga catctctttg tcggctttca gcttcaatcg ttttgaaaca tcgagcaaaa
    29881 tggcccgata caatttaccg tgtccgcgca gtttgttggc gatactatcg ccaccaaaat
    29941 gctgtaattc tccagcaatc agctgccagt tgcggcgatg ttgctcggga tgcccttcca
    30001 tcgatttaaa cagttcgttg cgcatcagta cgctggagag gcgagttttg cctttttcat
    30061 tatgggtgag caatcgggcg aaatttgcca actgttcctc actacaatgc tgaagaaaat
    30121 ccagatctga atcattcagg taattaacat tcattttttg tggtttctat attctggcgt
    30181 tagtcgtcgc cgataatttt cagcgtggcc atatccgatg agttcaccgt atgaccggaa
    30241 aaggtgattt ttgagacgca gcgtttattg tcgttatcac tgttaatgtt gatccagtca
    30301 gtggtttgcc cttcttttat ttctgaagga atattcagac tctgactggc gctacgggcg
    30361 gctttgaaat aaaccgatgc accgcttaac tgtaaatcgc catggtcggc agagagttgt
    30421 atgcgtttca caatgcgaca aacaggaagt ttcagcgcca gatcgttggt ttcgttacgc
    30481 ggcattgcaa tgacgccgag gagtttatgg tcgtttgcct gcgccgtgca gcacagcatc
    30541 aggctaatcg ccaggctggc ggaaatcgta aaaacggatt tcataaggat tctcttagtg
    30601 ggaagaggta gagggatgaa tacccactag tttactgctg ataaagagaa gattcaggta
    30661 cgtaatcttt tctttttatt acaatttttt gatgaatgcc ttggctgcga ttcattcttt
    30721 atatgaataa aattgctgtc aattttacgt cttgtcctgc catatcgcga aatttctgcg
    30781 caaaagcaca aaaaattttt gcatctcccc cttgatgacg tgggttacga ccccatttag
    30841 tagtcaaccg cagtgagtga gtctgcaaaa aaatgaaatt gggcagttga aaccagacgt
    30901 ttcgccccta ttacagactc acaaccacat gatgaccgaa tatatagtgg agacgtttag
    30961 atgggtaaaa taattggtat cgacctgggt actaccaact cttgtgtagc gattatggat
    31021 ggcaccactc ctcgcgtgct ggagaacgcc gaaggcgatc gcaccacgcc ttctatcatt
    31081 gcctataccc aggatggtga aactctggtt ggtcagccgg ctaaacgtca ggcagtgaca
    31141 aacccgcaaa acaccctgtt tgcgattaaa cgcctgattg gccgccgctt ccaggacgaa
    31201 gaagtacagc gtgatgtttc catcatgccg ttcaaaatta ttgctgctga taacggcgac
    31261 gcatgggtcg aagttaaagg ccagaaaatg gcaccgccgc agatttctgc tgaagtgctg
    31321 aaaaaaatga agaaaaccgc tgaagattac ttgggtgaac cggtaactga agctgttatc
    31381 accgtaccgg catactttaa cgatgctcag cgtcaggcaa ccaaagacgc aggccgtatc
    31441 gctggtctgg aagtaaaacg tatcatcaac gaaccgaccg cagctgcgct ggcttacggt
    31501 ctggacaaag gtactggcaa ccgtactatc gcggtttatg acctgggtgg tggtactttc
    31561 gatatttcta ttatcgaaat cgacgaagtt gacggcgaaa aaaccttcga agttctggct
    31621 accaacggtg atacccacct gggtggtgaa gacttcgaca gccgtctgat caactacctg
    31681 gttgaagaat tcaagaaaga tcagggcatt gacctgcgca acgatccgct ggcaatgcag
    31741 cgcctgaaag aagcggcaga aaaagcgaaa atcgaactgt cttccgctca gcagaccgac
    31801 gttaacctgc cgtacatcac tgcagatgcg accggtccga aacacatgaa catcaaagtg
    31861 actcgtgcga aactggaaag cctggttgaa gatctggtaa accgttccat tgagccgctg
    31921 aaagttgcac tgcaggacgc tggcctgtcc gtatctgata tcgacgacgt tatcctcgtt
    31981 ggtggtcaga ctcgtatgcc aatggttcag aagaaagttg ctgagttctt tggtaaagag
    32041 ccgcgtaaag acgttaaccc ggacgaagct gtagcaatcg gtgctgctgt tcagggtggt
    32101 gttctgactg gtgacgtgaa agacgtactg ctgctggacg ttaccccgct gtctctgggt
    32161 atcgaaacca tgggcggtgt gatgacgacg ctgatcgcga aaaacaccac tatcccgacc
    32221 aagcacagcc aggtgttctc taccgctgaa gacaaccagt ctgcggtaac catccatgtg
    32281 ctgcagggtg aacgtaaacg tgcggctgat aacaaatctc tgggtcagtt caacctggat
    32341 ggtatcaacc cggcaccgcg cggcatgccg cagatcgaag ttaccttcga tatcgatgct
    32401 gacggtatcc tgcacgtttc cgcgaaagat aaaaacagcg gtaaagagca gaagatcacc
    32461 atcaaggcgt cttctggtct gaacgaagat gaaatccaga aaatggtacg cgacgcagaa
    32521 gctaacgccg aagctgaccg taagtttgaa gagctggtac agactcgcaa ccagggcgac
    32581 catctgctgc acagcacccg taagcaggtt gaagaagcag gcgacaaact gccggctgac
    32641 gacaaaactg ctatcgagtc tgcactgact gcactggaaa ctgctctgaa aggtgaagac
    32701 aaagccgcta tcgaagcgaa aatgcaggag ctggcacagg tttcccagaa actgatggaa
    32761 atcgcccagc agcaacatgc ccagcagcag actgccggtg ctgatgcttc tgcaaacaac
    32821 gcgaaagatg acgatgttgt cgacgctgaa tttgaagaag tcaaagacaa aaaataatcg
    32881 ccctataaac gggtaattat actgacacgg gcgaagggga atttcctctc cgcccgtgca
    32941 ttcatctagg ggcaatttaa aaaagatggc taagcaagat tattacgaga ttttaggcgt
    33001 ttccaaaaca gcggaagagc gtgaaatcaa aaaggcctac aaacgcctgg ccatgaaata
    33061 ccacccggac cgtaaccagg gtgacaaaga ggccgaggcg aaatttaaag agatcaagga
    33121 agcttatgaa gttctgaccg actcgcaaaa acgtgcggca tatgatcagt atggtcatgc
    33181 tgcgtttgag caaggcggta tgggcggcgg cggttttggc ggcggcgcag acttcagcga
    33241 tatttttggt gacgttttcg gcgatatttt tggcggcgga cgtggtcgtc aacgtgcggc
    33301 gcgcggtgct gatttacgct ataacatgga gctcaccctc gaagaagctg tacgtggcgt
    33361 gaccaaagag atccgcattc cgactctgga agagtgtgac gtttgccacg gtagcggtgc
    33421 aaaaccaggt acacagccgc agacctgtcc gacctgtcat ggttctggtc aggtgcagat
    33481 gcgtcagggt ttctttgccg tgcagcagac ctgtccacac tgtcagggcc gcggtacgct
    33541 gatcaaagat ccgtgcaaca aatgtcatgg tcatggtcgt gttgagcgca gcaaaacgct
    33601 gtccgttaaa atcccggcag gggtggacac tggagaccgc atccgtcttg cgggcgaagg
    33661 tgaagcgggc gagcatggcg caccggcagg cgatctgtac gttcaggttc aggttaaaca
    33721 gcacccgatt ttcgagcgtg aaggcaacaa cctgtattgc gaagtcccga tcaacttcgc
    33781 tatggcggcg ctgggtggcg aaatcgaagt accgaccctt gatggtcgcg tcaaactgaa
    33841 agtgcctggc gaaacccaga ctggtaagct gttccgtatg cgcggtaaag gcgtcaagtc
    33901 tgtccgcggt ggcgcacagg gtgatttact gtgccgcgtt gtcgtcgaaa caccggtagg
    33961 cctgaacgag aagcagaaac agctgctgca agagctgcaa gaaagcttcg gtggcccaac
    34021 cggtgagcac aacagcccgc gctcaaagag cttctttgat ggtgtgaaga agttttttga
    34081 cgacctgacc cgctaacctc cccaaaagcc tgcccgtggg caggcttggg taaaattagg
    34141 gtgcgttgaa gatatgcgag cacctgaaaa gtggcgggga tc
//
LOCUS       DAPHBJ010000028        33615 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.028, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000028 DAPHBJ010000000
VERSION     DAPHBJ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.028"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            9..245
                     /locus_tag="RHZ76_001241"
     CDS             9..245
                     /locus_tag="RHZ76_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HDV2811911.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            248..1105
                     /locus_tag="RHZ76_001242"
     CDS             248..1105
                     /locus_tag="RHZ76_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV2811912.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            1105..3116
                     /locus_tag="RHZ76_001243"
                     /pseudo
     CDS             1105..3116
                     /locus_tag="RHZ76_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(3117..3638)
                     /gene="sodC"
                     /locus_tag="RHZ76_001244"
     CDS             complement(3117..3638)
                     /gene="sodC"
                     /locus_tag="RHZ76_001244"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HDV2811913.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(3719..4615)
                     /locus_tag="RHZ76_001245"
     CDS             complement(3719..4615)
                     /locus_tag="RHZ76_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HDV2811914.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(4664..4903)
                     /locus_tag="RHZ76_001246"
     CDS             complement(4664..4903)
                     /locus_tag="RHZ76_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HDV2811915.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            5006..5605
                     /gene="nemR"
                     /locus_tag="RHZ76_001247"
     CDS             5006..5605
                     /gene="nemR"
                     /locus_tag="RHZ76_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HDV2811916.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            5642..6739
                     /gene="nemA"
                     /locus_tag="RHZ76_001248"
     CDS             5642..6739
                     /gene="nemA"
                     /locus_tag="RHZ76_001248"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HDV2811917.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            6820..7227
                     /gene="gloA"
                     /locus_tag="RHZ76_001249"
     CDS             6820..7227
                     /gene="gloA"
                     /locus_tag="RHZ76_001249"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDV2811918.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            7330..7977
                     /gene="rnt"
                     /locus_tag="RHZ76_001250"
     CDS             7330..7977
                     /gene="rnt"
                     /locus_tag="RHZ76_001250"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HDV2811919.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            8070..12686
                     /locus_tag="RHZ76_001251"
     CDS             8070..12686
                     /locus_tag="RHZ76_001251"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDV2811920.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQPQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDVFIAHSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEKA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIEEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(12737..13084)
                     /gene="grxD"
                     /locus_tag="RHZ76_001252"
     CDS             complement(12737..13084)
                     /gene="grxD"
                     /locus_tag="RHZ76_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HDV2811921.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            13418..14233
                     /gene="mepH"
                     /locus_tag="RHZ76_001253"
     CDS             13418..14233
                     /gene="mepH"
                     /locus_tag="RHZ76_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HDV2811922.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            14361..14942
                     /gene="sodB"
                     /locus_tag="RHZ76_001254"
     CDS             14361..14942
                     /gene="sodB"
                     /locus_tag="RHZ76_001254"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HDV2811923.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(15088..16257)
                     /locus_tag="RHZ76_001255"
     CDS             complement(15088..16257)
                     /locus_tag="RHZ76_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2811924.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(16423..16512)
                     /gene="ynhF"
                     /locus_tag="RHZ76_001256"
     CDS             complement(16423..16512)
                     /gene="ynhF"
                     /locus_tag="RHZ76_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HDV2811925.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            16811..17836
                     /gene="purR"
                     /locus_tag="RHZ76_001257"
     CDS             16811..17836
                     /gene="purR"
                     /locus_tag="RHZ76_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HDV2811926.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(17833..18765)
                     /locus_tag="RHZ76_001258"
     CDS             complement(17833..18765)
                     /locus_tag="RHZ76_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2811927.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            18878..20089
                     /locus_tag="RHZ76_001259"
     CDS             18878..20089
                     /locus_tag="RHZ76_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HDV2811928.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            20380..21528
                     /gene="cfa"
                     /locus_tag="RHZ76_001260"
     CDS             20380..21528
                     /gene="cfa"
                     /locus_tag="RHZ76_001260"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HDV2811929.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSESHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(21568..22209)
                     /gene="ribE"
                     /locus_tag="RHZ76_001261"
     CDS             complement(21568..22209)
                     /gene="ribE"
                     /locus_tag="RHZ76_001261"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDV2811930.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            22424..23797
                     /gene="mdtK"
                     /locus_tag="RHZ76_001262"
     CDS             22424..23797
                     /gene="mdtK"
                     /locus_tag="RHZ76_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HDV2811931.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALAEKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(23838..25094)
                     /locus_tag="RHZ76_001263"
     CDS             complement(23838..25094)
                     /locus_tag="RHZ76_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDV2811932.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFSVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQISAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            25402..25478
                     /locus_tag="RHZ76_001264"
     tRNA            25402..25478
                     /locus_tag="RHZ76_001264"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25436..25438,aa:Val,seq:gac)
     gene            25483..25559
                     /locus_tag="RHZ76_001265"
     tRNA            25483..25559
                     /locus_tag="RHZ76_001265"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25517..25519,aa:Val,seq:gac)
     gene            25667..25972
                     /locus_tag="RHZ76_001266"
     CDS             25667..25972
                     /locus_tag="RHZ76_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDV2811933.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            26098..27702
                     /locus_tag="RHZ76_001267"
     CDS             26098..27702
                     /locus_tag="RHZ76_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HDV2811934.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARKQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFHGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(27714..28526)
                     /locus_tag="RHZ76_001268"
     CDS             complement(27714..28526)
                     /locus_tag="RHZ76_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2811935.1"
                     /translation="MIITQADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALVYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(28530..29315)
                     /locus_tag="RHZ76_001269"
     CDS             complement(28530..29315)
                     /locus_tag="RHZ76_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HDV2811936.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQTHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(29312..29980)
                     /locus_tag="RHZ76_001270"
     CDS             complement(29312..29980)
                     /locus_tag="RHZ76_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDV2811937.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(30044..30682)
                     /locus_tag="RHZ76_001271"
     CDS             complement(30044..30682)
                     /locus_tag="RHZ76_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HDV2811938.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(30695..32797)
                     /locus_tag="RHZ76_001272"
     CDS             complement(30695..32797)
                     /locus_tag="RHZ76_001272"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HDV2811939.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNRPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            complement(32818..33444)
                     /locus_tag="RHZ76_001273"
     CDS             complement(32818..33444)
                     /locus_tag="RHZ76_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HDV2811940.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         8057 a         8878 c         8283 g         8397 t
ORIGIN      
        1 tctactttgt gaagtttatg ctcaatgcaa caggattgcc cttacaagat ctggtgttcg
       61 gtgcgtccgt ctactttcct ccgtttttca aagccttcgc gtttggattc gtcatctggc
      121 ttgtcgtaca ccgcctgctt cgtggctgga tctacgccgg tgacatctgg catcccttgt
      181 taatggattt atcgctgttt gcgatttgcg tttgccttgc tctggcaata ctgattgcgt
      241 ggtaactatg tcaattaaaa caattaagta tttctcaaca atcattgtag cggtagttgc
      301 ggttcttgcc ggatggtggc tgtggaatta ttacatgcaa tctccgtgga cacgcgacgg
      361 gaaaatacgc gcagaacagg tttctatcac cccacaggtg tcgggacgta ttgttgagct
      421 gaatataaaa gacaaccagc tggttaacgc gggggatctt ctgctcacga tcgataaaac
      481 gccctttcag atcgccgagc tgaacgcaca ggctcagctg gcgaaagcgc aatctgacct
      541 cgcaaaagcc aataacgaag cgaatcgtcg tcgtcatctc tcacaaaatt ttatttctgc
      601 cgaagaactc gataccgcta acctcaatgt taaagcgatg caggccagtg ttgatgccgc
      661 acaggcgacg ctgaaacagg cacaatggca actggcgcaa acggaaattc gcgccccggt
      721 gagtggatgg gtgactaacc ttactacccg catcggcgat tacgctgata ccgggaaacc
      781 gctgtttgcc cttgtcgaca gccactcgtt ttacgtcatt ggttattttg aagaaaccaa
      841 gttgcgccat atccgcgaag gtgcaccagc acaaattacc ctctatagtg ataacaaaac
      901 gttacagggt cacgtttcca gtatcggtcg ggcgatttat gatcagagcg ttgaaagtga
      961 ctcaagcctt attccggatg ttaaacctaa cgtcccctgg gttcgactcg cccaacgcgt
     1021 tcccgtccgc tttgcgctgg ataaagtccc aggcgatgtc acgttggtgt cgggcaccac
     1081 ctgtagcatt gccgtaggtc aataatgaac gcatcgtcat ggtccttgcg caatttgccc
     1141 tggttcaggg ccacgctggc gcaatggcgt tatgcgttac gcaataccat tgccatgtgt
     1201 ctggcgctga cggttgccta ttatttaaat ctggatgaac cctattgggc gatgacctcg
     1261 gctgcagtgg ttagctttcc caccgttggc ggtgttatca gcaaaagcct cggacgcatc
     1321 gctggcagtt tgctcggagc cattgcggca ctgcttcttg ccgggcatac gctcaatgag
     1381 ccgtggtttt ttctattgag catgtcggcg tggcttggct tttgtacctg ggcctgtgcg
     1441 cacttcacga ataacgtcgc gtatgcattt caactggcgg gctacacggc tgccatcatc
     1501 gcctttccga tggttaatat tactgaggcc agccagctgt gggatatcgc tcaggcgcgc
     1561 gtttgcgagg tgattgtcgg cattttgtgc ggcggcatga tgatgatgat cctgcctagc
     1621 agttccgatg ctacagccct tttaaccgca ttgaaaaaca tgcacgcccg actacttgaa
     1681 catgccagtt tactctggca gcctgaaaca accgatgcca ttcgtgcagc acatgaaggg
     1741 gcgattgggc agatactgac catgaatttg ctgcgtatcc aggctttctg gagccactat
     1801 cgttttcgcc agcaaaacgc gcgccttaat gcgctgctcc accagcaatt acgtatgacc
     1861 agtgtcatct ccagcctgcg acgtatgttg ctcaactggc cctcaccgcc aggtgccaca
     1921 cgagaaattc tcgaacagtt gctgacggcg ctcgccagtt cgcaaacaga tgtttacacc
     1981 gtcgcacgta ttatcgcccc gctacgcccg accaacgtcg ccgactatcg gcacgtcgcc
     2041 ttctggcagc gactacgtta tttttgccgc ctttatctgc aaagtagtca ggaattacat
     2101 cgtctgcaaa gcggtgtaga tgatcatacc agactcccac ggacatccgg cctggctcgt
     2161 cataccgata acgccgaagc tatgtggagc gggctgcgta cattttgtac gttgatgatg
     2221 attggcgcat ggagtattgc ttcgcaatgg gatgccggtg ccaatgcatt aacgctggca
     2281 gcaattagct gcgtactcta ctccgccgtc gcagcaccgt ttaagtcgtt gtcacttctg
     2341 atgcgcacgc tggtgttact ttcgctattc agctttgtgg tcaaatttgg tctgatggtc
     2401 cagattagcg atctgtggca atttttactg tttctctttc cactgctggc gacaatgcag
     2461 cttcttaaat tgcagatgcc aaaatttgcc gcattgtggg gcaactgatt gtttttatgg
     2521 gttcttttat cgctgtcact aatcccccgg tgtatgattt tgctgatttt cttaacgata
     2581 atctggcaaa aatcgttggc gtcgcgttgg cgtggttagc gttcgccatt ctgcgtccag
     2641 gatcggatgc tcgtaaaagc cgccgccata ttcgcgcgct gcgccgggat tttgtcgatc
     2701 agctaagccg ccatccaaca ctgagtgaaa gcgaatttga atcgctcact tatcatcacg
     2761 tcagtcagtt gagtaacagc caggatgcgc tggctcgccg ttggttatta cgctggggtg
     2821 tagtgctgct gaactgttct catgttgtct ggcaattgcg cgactgggaa tcgcgttccg
     2881 atccgttatc gcgagtacgg gataactgta tttcactgtt gcggggagtg atgagtgagc
     2941 gtggcgttca gcaaaaatca ctggcggcca cacttgaaga attacagcgg atttgcgaca
     3001 gccttgcccg tcatcatcaa cctgccgccc gtgagctggc ggcaattgtc tggcggctgt
     3061 actgctcgct ttcgcaactt gagcaagcac caccgcaagg tacgctggcc tcttaattac
     3121 ttaattacac cacaggcata gcgttcaccg ccaccgccca gcggtttagg ttgatcggac
     3181 atattatcgc cgccaacgtg gaccatcagc gctttgtctt tgatttcatc cagtgatttc
     3241 agacgaggcg cgatgacggc atcggtagct ttgccgtcat tattgacgac cagtgcaggc
     3301 agatcgccta aatgcccggc accttctggc ccttcatgtt taccggtatt ttgtggatca
     3361 agatgcccgc ctgcggattc cgcggcgctg gctttgccat ctttggtggc tggctggcag
     3421 cttcctttgg catgaatatg gaagccatgt tcaccggggg gtaatgcttt cagatcgggc
     3481 gaaaactcca gacctttatc ggtttcagta atggtgacgc taccaattga ctgccctacc
     3541 ccttgcgacg tgacgaggtt catctcgact ttttcactgg cagcttgtgc gccggttgca
     3601 acaaccagcg ccagaatagc cagactaaaa cgtttcatag gacctccgtt cattgtgcat
     3661 tcctgaataa gtgtaaacca gtgacacatt tttgaacggc ggctattcct aaaagtgctt
     3721 acggtacgtc gtaccccagt gccgctttac ggatacgaaa ccattgttga cgggtcattt
     3781 tcagtgtttc tgcttcgaca gctgcccgta cgcgctcaat tttacctgaa ccgataattg
     3841 gcagcggctg cgatggtaaa cgtaataccc aggcgtaaac cacctgttca atcgagcccg
     3901 cgtttaactc ctctgccacc acagccagtt catcacgcag cggctggaaa taatcatcat
     3961 taaacagacg accaccacca aggcaggacc acgccatcgg acgaacacgc agttgttgta
     4021 gttggtcgag cgtgccatcc agcagtaacg gctgatgcac cggggatatt tccacctgat
     4081 tagtggcaag ggtaaacggc agacgtgatt gcaacagggc aaattgcgca ggcgtaaagt
     4141 tcgatacgcc aaaatgacgc actttgccgc tctgatgcag atgtttgaac gcgtccgcca
     4201 cttcatcggc atccattaac gggtctggtc ggtggattaa cagcaaatcc agatgatcgg
     4261 tcgcgagatt aattagcgac tgttcggcgc tcttaatgat gtgatcgcgg tcagtgatgt
     4321 aatgaccaat gacgttttct tcacgcgcgg tcgtcgcgat accgcattta ctgacgattt
     4381 ccatccgttc acgcaggtga ggtgccagtt tcagtgcctc gccaaacgcc gcttcgcact
     4441 gatagccacc ataaatatca gcatggtcca cggtggtcac gccgagatcc agatgctctt
     4501 caataaaact gaccagctgg cgggcggaca tattccagtc catcaatcgc cagtagccca
     4561 tcacaaaacg ggaaaactcc gggccttgcg gcgcaatagt aatacgctga accataatcg
     4621 cttcctctta tcagatatga gaggagtata cgcaagatta ggttcaaaag agtgatggtt
     4681 gctccggttc gtctgatgac gctggcttat ttgcgcgtaa tttgcgcatt aatcgctgcc
     4741 gacaaaggcg cagcacctct tgtttttcgc catcgctcat tttattccag ttaaaacgct
     4801 catcacgact acgaaaacag ccgcgacaaa acccgcgttc gtcagactgg caaattcccc
     4861 ggcacgggct ctggacggga aagaactcta attgctccgc cacttcgccc tcctcagata
     4921 agattattac cattattgaa gctgttaatg tccaaagtag caactttgct tgcactagac
     4981 cgactggtct actacactcc aacgcatgaa caaacacacc gaacatgata ctcgcgaaca
     5041 tctcctggcg acgggcgagc aactttgcct gcaacgtgga ttcaccggga tggggctaag
     5101 cgaattacta aaaaccgctg aagtgccgaa agggtccttc tatcactact ttcgctctaa
     5161 agaagcgttt ggcgttgcca tgcttgagcg tcattacgcc gcatatcacc agcgactgac
     5221 tgagttgctg caatccggcg aaggtaacta ccgcgaccgc atactggctt attaccagca
     5281 aacactgaac cagttttgcc aacatggaac catcagtggt tgcctgacag taaaactctc
     5341 tgccgaagtg tgcgatctgt cagaagatat gcgcagcgcg atggataaag gtgctcgcgg
     5401 cgtgatcgcc ctgctctctc aggcgctgga aaatggccgt gagaaccatt gtttaacctt
     5461 ttgtggcgaa ccgctgcaac aggcacaagt gctttacgca ctgtggcttg gcgcgaatct
     5521 gcaggccaaa atttcgcgca gtttcgagcc actggaaaac gcgctggccc atgtaaaaaa
     5581 cattattgcg acgcctgccg tttagcaggc attttttatc accagacgac cgggagcctt
     5641 tatgtcatct gaaaaactgt attccccact gaaagtgggc gcgatcacgg cggcaaaccg
     5701 tatttttatg gcaccgctga cgcgtctgcg cagtattgaa ccgggtgaca ttcctacccc
     5761 gttgatggcg gaatactatc gccaacgtgc cagtgccggt ttgattatta gtgaagccac
     5821 gcaaatttct gcccaggcaa aaggatatgc aggtgcgcct ggcatccata gtccggagca
     5881 aattgccgca tggaaaaaaa tcaccgctgg cgttcatgct gaaaatggtc atatggccgt
     5941 gcagctgtgg cacaccggac gcatttctca cgccagcctg caacctggcg gtcaggcacc
     6001 ggtagcgcct tcagcactta gcgcgggaac acgtacttct ctgcgcgatg aaaatggtca
     6061 ggcgatccgt gttgaaacat ccatgccgcg tgcgcttgaa ctggaagaga ttccaggtat
     6121 cgtcaatgat ttccgtcagg ccattgctaa cgcgcgtgaa gccggttttg atctggtaga
     6181 gctccactct gctcacggtt atttgctgca tcagttcctt tctccttctt caaaccatcg
     6241 taccgatcag tacggcggca gcgtggaaaa tcgcgcacgt ttggtactgg aagtggtcga
     6301 tgccgggatt gaagaatggg gtgccgatcg cattggcatt cgcgtttcgc caatcggtac
     6361 tttccagaac acagataacg gcccgaatga agaagccgat gcactgtatc tgattgaaca
     6421 actgggtaaa cgcggcattg cttatctgca tatgtcagaa ccagattggg cggggggtga
     6481 accgtatact gatgcgttcc gcgaaaaagt acgcgcccgt ttccacggtc cgattatcgg
     6541 cgcaggtgca tacacagtag aaaaagctga aacgctgatc ggcaaagggt taattgatgc
     6601 ggtggcattt ggtcgtgact ggattgcgaa cccggatctg gtcgcccgct tgcagcgcaa
     6661 agctgagctt aacccacagc gtgccgaaag tttctacggt ggcggcgcgg aaggctatac
     6721 cgattacccg acgttgtaat ccaacattgc gagcggcgta aagccgccgc tatactaaaa
     6781 caacattttg aatctgttag ccattttgag gataaaaaga tgcgtcttct tcataccatg
     6841 ctgcgcgttg gcgatttgca acgctccatc gatttttata ccaaagtgct gggcatgaaa
     6901 ctgctgcgta ccagcgaaaa cccggaatac aaatactcac tggcgtttgt tggctacggc
     6961 ccggaaaccg aagaagcggt gattgaactg acctacaact ggggcgtgga taaatacgaa
     7021 ctcggcactg cttatggtca catcgcgctt agcgtagata acgccgctga agcgtgcgaa
     7081 aaaatccgtc aaaacggggg taacgtgacc cgtgaagcgg gtccggtaaa aggcggtact
     7141 acggttatcg cgtttgtgga agatccggac ggttacaaaa ttgagttaat cgaagagaaa
     7201 gacgccggtc gcggtctggg caactaatct cctgccgggc gtgaactcat cgcgcccgca
     7261 tctttactgc atcgacaagt aatatttgtc ataatgcgcg ctgcaattta tccgtattaa
     7321 gagaatcaga tgtccgataa cgctcaactt accggtctgt gcgaccgttt tcgtggtttt
     7381 tatcctgttg tgatcgatgt tgaaacagcc ggatttaacg ccaaaaccga tgcgctgctt
     7441 gagattgccg ccatcaccct gaaaatggat gaacaaggct ggctgatgcc ggacaccaca
     7501 ttacatttcc acgtcgaacc atttgtcggc gcaaatttgc aaccagaagc cctcgccttc
     7561 aacggcattg acccgaacga tcccgatcgc ggcgcggtca gcgaatacga ggcgctgcac
     7621 gaaattttta aagttgtacg taaaggtatt aaagcgagcg gctgtaaccg cgccattatg
     7681 gtggcgcaca atgccaattt tgatcacagc tttatgatgg ccgccgcaga acgcgcctca
     7741 ctgaaacgta acccgttcca ccctttcgcc acttttgaca ctgctgcact ggccgggctg
     7801 gcactcggac aaaccgtatt gtcaaaggct tgccagaccg ctggcatgga cttcgacagc
     7861 acccaggcgc actccgcgct gtacgacacc gaacgcactg ctgtgctgtt ttgtgaaatc
     7921 gtcaaccgct ggaaacgtct gggaggctgg ccgctacctg ccgccgaaga ggtgtaatcg
     7981 agtcgatgcc tgatgacatg caatgattca ggcatctata gtgaggctat tccacgcatc
     8041 ctgcatgata ttcacgggga atagcgttaa tggcagataa tccagaccct tcatcgctcc
     8101 tgccggacgt gttttcaccg gcgacccgcg actggtttct tcgcgccttt aaacagccga
     8161 ccgctgtcca gccgcaaacc tggcatgtgg cggcgcgaag cgaacatgcg ctggtgattg
     8221 caccgaccgg ctccgggaaa acgctggcag catttctcta cgccctcgat cggctcttcc
     8281 gcgaaggcgg cgaagatacc cgcgaggcgc ataagcgtaa aacctcacgc atcctctata
     8341 tttcaccgat aaaagccctg ggcaccgacg ttcagcgcaa cttgcagatc ccgttgaagg
     8401 gtattgccga tgaacggcgg cggcgcggcg aaacggaagt caatcttcgc gtagggatcc
     8461 gtactggcga tacgcctgca caggaacgca gcaaactcac ccgtaatccg ccggatattc
     8521 tgatcaccac acccgaatca ctctatctga tgctgacctc ccgcgcgcgc gaaacgctac
     8581 gcggcgtcga aacggtaatt attgatgaag tccacgcggt agcgggcagt aaacgtggtg
     8641 cgcatctggc gttaagtctg gagcggctcg atgcgctgct ccacacctca gcacagcgaa
     8701 ttggcctttc tgccactgtg cgctcagcca gcgatgtggc agcatttctt ggtggcgatc
     8761 gcccggttac ggtagtcaac ccgcccgcaa tgcgccatcc gcagatacga attgtcgtac
     8821 cggtcgccaa tatggatgat gtctcatcgg tcgccagcgg caccggcgaa gacagccatg
     8881 ccggccggga aggctccatc tggccatata ttgaaacggg tatccttgat gaagtgttgc
     8941 gccatcgctc gaccattgtc tttactaatt cacgcgggct ggcggaaaaa ctgacggcac
     9001 gattaaatga gctttacgcc gcacgcttac agcgttcccc gtctatcgcc gttgatgcgg
     9061 cccatttcga gtcgacctcc ggcgcaacct ctaaccgtgt acaaagtagc gacgttttta
     9121 ttgcccactc acaccacggc tccgtctcta aagaacaacg agcaatcacc gaacaggcgc
     9181 tgaaatcggg tgaattacgc tgcgtggtcg caacctccag tcttgaactg gggattgata
     9241 tgggcgcggt ggatctggtg attcaggtgg caacgccgct ttctgttgcc agtgggttac
     9301 aacgcattgg tcgcgccgga catcaggttg gcggtgtatc taaagggctg tttttccccc
     9361 gtacccggcg tgatttagtc gattccgcag tcattgtaga gtgtatgttc gcaggcaggc
     9421 tggaaaacct gacaccaccg cataatcctc tcgacgtcct tgcgcagcaa accgttgccg
     9481 ccgcggcgat ggatgcatta caggtagacg aatggtactc ccgcgtacgc cgtgccgcac
     9541 cgtggaaaga tctgccaaga cgtgtttttg acgccacgct ggatatgctt tccgggcgct
     9601 atccctctgg cgatttttct gcttttcgcc ccaaactggt ctggaacagg gagaccggga
     9661 tattgaccgc ccgacctggc gctcaattgt tggcggttac cagcggcggc accattccgg
     9721 atcgtggcat gtatagcgtg ttattacccg aaggtgaaga aaaggccggt tcgcggcggg
     9781 tgggtgaact ggatgaggag atggtatatg agtcgcgggt gaacgacatt atcactctcg
     9841 gcgctacctc atggcggatc cagcaaatca cccgcgatca ggtgattgtg actcctgctc
     9901 cgggtcgttc tgcccggctc cccttctggc gtggtgaagg taacggacgt ccggctgaat
     9961 taggcgagat gatcggcgat tttcttcatt tgctggcgga tggcgcgttc ttttccggga
    10021 ctattccccc gtggctggca gaagaaaata cgatcgccaa tattcagggg ttgattgagg
    10081 agcagcgcaa cgcgacgggc atcgttccgg ggagtcgcca tctggtcctc gaacggtgcc
    10141 gtgatgaaat tggtgactgg cgtattattt tgcactctcc ctatggaaga cgggtgcatg
    10201 aaccctgggc gctggcgatt gccgggcgaa tacatgcgct atggggcgct gacgcgtcgg
    10261 tggtcgccag tgatgacggc attgttgcac gtattcctga caccgatggt aaattgcccg
    10321 atgccgcgat ttttttgttt gaaccagaaa agttgctgca aattgtccgc gaggcggtag
    10381 gcagctcggc acttttcgcc gcccgttttc gcgaatgcgc cgcgcgggca ttattaatgc
    10441 cggggcgcac tccgggccat cgcaccccgc tttggcaaca acggctgcgc gccagtcagt
    10501 tgctggaaat cgctcaggga tatccggatt ttccggtcat tctcgaaacc ctacgcgaat
    10561 gtctgcaaga tgtttatgat cttcccgcac tggaacgttt gatgcgtcgc ctgaacggtg
    10621 gcgaaattca aatatccgat gtaacgacca ctacgccctc gcctttcgcc acaagtttat
    10681 tgttcggcta tgtcgcggaa tttatgtacc agagcgacgc cccgctggca gagcgccggg
    10741 catccgtact gtcgctggac agcgagttac tgcgcaatct actcggacag gtcgatccgg
    10801 gggaattact cgacccgcag gtcattcgcc aggtggaaga agagttgcaa cgactggctc
    10861 ctggcagaag agcgaaaggt gaagaaggat tgttcgacct gctgcgcgaa ctggggccaa
    10921 tgaccgttga agacctggcg caacggcata caggcagcag tgaagaggtt gcgtcgtatc
    10981 tggaaaatct tcttgcagta aaacgaatct tcccagcgat gattagcgga caggagcgtc
    11041 ttgcctgtat ggatgatgcc gccaggctgc gtgatgccct cggcgtacga ctaccagagt
    11101 cattgccaga gatttattta catagagtca gttacccgct tcgcgacctc tttctgcgct
    11161 atctccgggc tcatgctctg gtcacggctg aacaactggc tcatgagttt agtctcggta
    11221 ttgccattgt cgaagagcag cttcagcaac tgcgtgaaca gggtctggtg atgaatctgc
    11281 aacaagacat ctgggtgagc gatgaagtat ttcgtcgtct gcgtttgcgc tcgctgcaag
    11341 ccgccagaga agcgacgcgt cccgttgcag ccacgaccta tgcgcgattg ctgctggaac
    11401 gtcagggcgt attacccgcc accgatggta gcccggcgct ctttgcctca acatcgccag
    11461 gcgtttatga gggcgtagat ggcgtgatgc gggtgatcga acagcttgcc ggagtcggtt
    11521 tacccgcctc actctgggaa agccagatcc tgcctgcccg cgtacgcgac tattcgtcag
    11581 aaatgctcga tgaattactg gcaaccggtg cggttatctg gtcggggcaa aaaaaactgg
    11641 gtgaagatga cggcctggtg gcactgcatc tacaggaata tgctgcagaa tcgttcactc
    11701 ccgccgaagc ggatcaggcg aatcgttcgg cgctgcaaca agcgatagtc gctgttctgg
    11761 ctgacggagg agcctggttt gcacaacaaa tcagccaacg gatacgcgac aaaatcggcg
    11821 aatcggttga tctctctgcc ctgcaagagg cgttatgggc gctggtctgg caaggcgtca
    11881 tcaccagcga catttgggca ccgttacgcg ccctcacccg cagcagttcc aacgcacgca
    11941 cctcaactcg ccgcagtcac cgggctcgtc gtggacgtcc tgtctatgcg caacccgtct
    12001 cgccgcgggt atcttacaac acaccaaatc tggctggacg ctggtcgtta ttgcaggtgg
    12061 agccactaaa cgataccgaa aggatgctgg cgctggcgga aaatatgctc gaccgctacg
    12121 gcatcatcag tcgtcaggcg gtgatagccg aaaatatccc tggcgggttt ccatcgatgc
    12181 aaacgctttg tcgaagtatg gaagactccg ggcgaattat gcgaggtcgt tttgtagaag
    12241 gtctgggtgg cgcgcaattc gctgaacgtc tgactattga ccgattgcgc gatctggcga
    12301 cacaagccac gcaaacgcgc cactatacac cagtggcgct ctctgccaac gatccggcta
    12361 atgtgtgggg aaatcttctg ccctggcctg cacatccggc aacgctggtt ccaacgcgtc
    12421 gggcgggtgc gctggtggtc gtttctggcg gcaaattgtt actctatctg gcgcaaggtg
    12481 gcaaaaaaat gctggtctgg caggaaaaag aggaattact cgccccagag gttttccacg
    12541 cgctgactac cgcactgcgt cgcgaaccac ggctgcgctt tacgctaaca gaagtgaatg
    12601 acctaccggt ccggcaaacg ccgatgttta cgctgctgcg tgaggcggga ttttcaagtt
    12661 cgccacaagg gctggattgg ggatagagaa aggactgacg gatgcccgtt cgcatccgtc
    12721 agtattgcag gacggattat tccgcgtccg gctcttcaga cttgtattta gcggcagttt
    12781 ctttgatcag ctgctgcagt tcgccacgct gatacatttc gatcacgata tcacaaccgc
    12841 cgaccagctc gccgtcaacc cacagttgcg ggaaggtcgg ccagttagca tatttcggca
    12901 gttccgcacg aatgtccgga ttctgcagaa tatcaacata ggcaaaacgt tcgccacatg
    12961 cggcaagcgc ctggactgcc tgggcagaga aaccgcagct cggcagtttc ggtgaacctt
    13021 tcatgtacag caggatcggg ttttcagcaa tctggcgttg gattttttcg atagtggtgc
    13081 tcattgtctt gcttcctcaa acttctttac ggcagtaatc tgacattgta gcgggtcagt
    13141 gcggcatcgg aaaataacat tttcatcacg cttttgctat tttatccctt tgctctattt
    13201 gttgcatttc aaatattcgt ttcttctctg atgccggcaa actggcgtat ttataaccat
    13261 ttttagcttt cactgctgca tttttttgcg ctcgccaacg aaacgtattt tttaacaata
    13321 aaagctatta actttctctt cttctatgca ttagaatcat caagttttgt aaatcagacg
    13381 caggcatgat agacctgcct taacagaggg acgctcagtg gcgcggataa accgtatttc
    13441 gatcacgctc tgtgctttac tttttaccac cctgccttta acgcctatgg cccatgcttc
    13501 aaagcaagcc agggagagtt ctgctaccac tcatatcacc aaaaaagcag ataaaaagaa
    13561 aagcacggca accaccaaaa agacccagaa aacagcgaaa aaagccgcca gtaaaagtac
    13621 gaccaaaagc aaaaccgctt cttccgttaa aaaatcttcc attaccgctt ctaaaaacgc
    13681 caaaactcgc agcaaacacg ccgtcaataa aacggcctca gccagcttta ccgaaaagtg
    13741 taccaaacgt aagggctata aatcgcattg tgtgaaagtc aaaaatgccg cgtcaggaac
    13801 tcttgccgac gcgcacaaag cgaaggtgca aaaagctacg aaagtggcaa tgaataaact
    13861 gatgcagcaa attggtaagc catatcgttg gggtggcagc tcaccgcgta ccggttttga
    13921 ttgcagcggc ctggtttatt acgcttataa agatttggtg aaaattcgta ttccgcgtac
    13981 ggcgaatgaa atgtatcacc tgcgtgatgc agcgccaatc gaacgtagtg aactgaaaaa
    14041 cggcgacctg gtctttttcc gtactcaggg acgcggcaca gccgatcatg tcggcgtgta
    14101 tgtcggcaac ggcaaattta ttcagtcacc gcgcacaggt caggaaattc aaatcacttc
    14161 tctcagtgaa gactactggc agcgccacta tgttggcgct cgtcgggtaa tgaccccaaa
    14221 aacacttcgc taaaacttta ccctgttgtt acggcaacag ggtaagttca tcttttgtct
    14281 caccttttaa tttgctaccc tatccatacg cacaataagg ctattgtacg tatgcaaatt
    14341 aataataaag gagagtagca atgtcattcg aattacctgc actaccatat gctaaagatg
    14401 ctctggcacc gcacatttct gcggaaacca tcgagtatca ctacggcaag caccatcaga
    14461 cttatgtcac taacctgaac aacctgatta aaggtaccgc gtttgaaggt aaatcactgg
    14521 aagagattat tcgcagctct gaaggtggcg tattcaacaa cgcagctcag gtctggaacc
    14581 atactttcta ctggaactgc ctggcaccga acgccggtgg cgaaccgact ggaaaagtcg
    14641 ctgaagctat cgccgcatct tttggcagct ttgccgattt caaagcgcag tttactgatg
    14701 cagcgatcaa aaactttggt tctggctgga cctggctggt gaaaaacagc gatggcaaac
    14761 tggctatcgt ttcaacctct aacgcgggta ctccgctgac caccgatgcg actccgctgc
    14821 tgaccgttga tgtctgggaa cacgcttatt acatcgacta tcgcaatgca cgtcctggct
    14881 atctggagca cttctgggcg ctggtgaact gggaattcgt agcgaaaaat ctcgctgcat
    14941 aataactgat ggcaaatgca gcattgcctg aagcgctacg cttaccatgc caacgcggat
    15001 gaggcgttaa cagcgcattc ggcaagataa atcgcacgtt gtcagcaact gtaacgcaga
    15061 aggttatcct tctgcgtttt tgtttaatta gctgttagca acgcaaactg tttcaggttg
    15121 ttttctggct gacataaaca ccagcaataa tgccagtccc gcgacaatcg ctcccatcac
    15181 cggcacaaag ctgtatccca gcccagcgga aattaccgca ccaccagcag ctgctcccag
    15241 cgcatttcca agattaaagg caccaatatt gactgatgaa gacagacctg gcgcttcact
    15301 ggcgacacgc atcacgcgca tctgtaacgg cggtacgacc gcaaaggttg ctgcgcccca
    15361 caccaccatg ctaatagctg cgccgaactc attgcgggcc aggaacggga ttgccagcat
    15421 aatcaccatc aacaacaaca aaaagccttt caacgtgccg ttaactgaac gatctgccag
    15481 tttgccgccg agatagttac cgatagagaa tccgacacca atcagcacca gcattgccgt
    15541 gacgaacacc ggtgttgcgt gggtaatact ttgcagtacc ggagagatat aggtgtagag
    15601 agtaaacatt gcaccagctc ccagtaccgt cgtcagcaat gcagacagca cctgcggacg
    15661 cattaatacc gccagctctt ttttcacttc aggtcgtgcc cctgcaccac ctttaggtaa
    15721 tgagaagaac agacttacca ttgaaatcac tcccagcccc gccgttgcca gaaatgacat
    15781 ccgccagccg atggtttcac ccaaccaggt cgccgccggc acgccaccga tatttgccag
    15841 ggttaacccc ataaacatag tggcaactgc gctggcctgt ttatgttttg gcaccacgct
    15901 tgcggccacg actgaaccca aaccaaaaaa tgctccgtga ttcaggctgg tcaaaatgcg
    15961 tgaaagcatc agggtcatat aatccggcgc gatggcggaa agaacgttgc ctagcgtgaa
    16021 aattgccatc aggaaaatca acgcactgcg gcgggcacga tgagaaagta gaagcgtcat
    16081 cagcggcgcg cccaccatta cgccaactgc ataggcactg attaacattc cggcagcggg
    16141 aatcgagaca tccacaccgc gcgcaatgac gggcaacaag cccattggcg agaactccgt
    16201 tgtcccgata ccaaacgcgc caatcgccag cgccagcaac ggatagttaa ttttcatgcc
    16261 ttatctccac ctcttcgcgt cattacgcga tattcattaa agtggcgaaa gcatgacagc
    16321 aatcacaaaa aaatgaaaat aacaaaaaga gaaaacactt ttgccatttt gctaacaaac
    16381 aggaaggaga tgcgagggag aacgcgctcc ctcgagagga aatcagtgca gcgcggcagt
    16441 caaacccacg gctacgatca aaccgaggac gataatcgtt gttaccagtg aaaatttaag
    16501 gtcggtgctc atcaagtttt ctcctttttt attaccacac aaaaagtgat attacgcatt
    16561 tttacacact gtgatgaaaa aatctcccgt catttataat gataagtgtt tttaccactt
    16621 ccccttttcg tcaagatcgg ccaaaattcc acgcttacac tatttgcgta ctggccattg
    16681 accccttcct gacgctccgt gtcgtttttc cggcgtaccg caacactttt gttgtgcgta
    16741 aggtgtgtaa aggcaaacgt ttaccttgcg attttgcagg agctgaagtt agggtctgga
    16801 gtgaaatgga atggcaacaa taaaagatgt agcgaaacga gcaaacgttt ccactacaac
    16861 tgtgtcacac gtgatcaaca aaacacgttt cgtcgctgaa gaaacgcgca acgccgtgtg
    16921 ggcagcgatc aaagaattac actactcccc tagcgcggtg gcgcgtagcc tgaaggttaa
    16981 ccacaccaag tctatcggtt tgctggcgac cagcagcgaa gcggcctatt ttgccgagat
    17041 cattgaagca gttgaaaaaa attgcttcca gaaaggttac accctgattc tgggtaatgc
    17101 gtggaacaat cttgagaaac agcgggctta tctgtcgatg atggcgcaaa aacgcgtcga
    17161 tggtctgctg gtgatgtgtt ctgagtaccc agagccgttg ctggcgatgc tggaagagta
    17221 tcgccatatc ccaatggtgg tgatggactg gggtgaagca aaagctgact tcaccgatgc
    17281 ggtcattgat aacgcgttcg aaggcggcta catggccggg cgttatctga ttgaacgcgg
    17341 tcaccgcgaa atcggcgtca tccccggccc gctggaacgt aacaccggcg caggccgcct
    17401 tgccggtttt atgaaggcga tggaagaagc gatgatcaag gtgccggaaa gctggattgt
    17461 tcagggtgac tttgaacccg aatctggtta tcgcgccatg cagcaaatac tgtcgcagcc
    17521 gcatcgccct actgccgtct tctgtggtgg cgatatcatg gcaatgggcg cactttgtgc
    17581 tgctgatgaa atgggtctgc gcgtcccgca ggatgtttcg ctgatcggtt atgataacgt
    17641 gcgcaacgcc cgctatttta cgccggcgct aaccacgatc caccagccaa aagattcgct
    17701 gggtgaaaca gcgttcaaca tgctgttgga tcgtatcgtc aacaaacgtg aagaaccgca
    17761 gtccattgaa gtgcatccgc gcttgattga acgccgctcc gtggctgacg gcccgttccg
    17821 cgactatcgt cgttaatcac ccgttgcggg agtctcttcc ggctcccgca gccactcctt
    17881 attcagcgtc tcactatcgc cgagatactc aagcagccag gttaacgcag gcgacatatc
    17941 attttgctgc catgtcagac aacatgccga atccggaaag gggttttcca gttctaatgc
    18001 cacccacttc ccctcattaa gccacggttt ggcgaaatgt gttggcacca tccctatgca
    18061 taatcctgcc gagatacagg ttgccgatga ttcccagtca ggcacgacaa ctcttttttg
    18121 gttatccagc aaccaagtaa tacgtttagg tagcgttcgc gaggtgtctt cgcgcaccaa
    18181 cgacggccag ttgcgcaacg tatcatcgct gaacgggcca tccatcaacg ccagcgggtg
    18241 gtggctggca acaacgcaac tccagcttag catccccata tcccggaagg cataacgacc
    18301 gcctaccgga atcgcgcgtg ttgcgccaat cgccagttcc acgcgcccat cggaaagcgc
    18361 atcccagaca ccgttaaaca cttcctgaaa gaccagaagt tcgacgtcgt caaaatggcg
    18421 ataaaaatca acgatcatct gccgtgtacg ttctggcctg acaatattat ccactgcgat
    18481 agctaactga ccgcgccagc cgttcgctat ctgctgacat tgctggcggg tgatctgcat
    18541 ttttttgaca acagagcgcc cttctttgag aaaccacgct ccggcagcgg tcagttccac
    18601 atcacggtgc cgtctttcaa agagcggcac cgccagccac tcttccagct gacgcacggt
    18661 atagctgacc gcagaaggaa cgcgatgcag ctcctgtgcc gcagcgctaa aactaccatt
    18721 acgcgctacc gcatcaacaa cttcgagtga atattctgac cacatagtct gcctgcaaaa
    18781 tttttgaaat cagtcatcaa atattaccgt ttcacaacac taatttcact ccctacactt
    18841 tgcggcggtg tttaattgag agatttagag aatatacatg caaccaggga aaagattttt
    18901 agtctggctg gcgggtttga gcgtactcgg ttttctggca accgatatgt atctgcctgc
    18961 tttcgccgcc atacaggccg acctgcaaac gcctgcgtct gctgtcagtg ccagccttag
    19021 tctgttcctt gccggttttg ccgcagccca gcttctgtgg gggccactct ccgaccgtta
    19081 tggtcgtaaa ccggtgttat taatcggcct gacaattttt gcgttaggta gtctggggat
    19141 gctgtgggta gaaaacgccg ccacgctgct ggtattgcgt tttgtacagg ctgtgggggt
    19201 ctgcgccgcg gcggttatct ggcaagcatt agtgacagat tattatcctt cacagaaagt
    19261 caaccgtatt tttgcgacca tcatgccgct ggtgggtctg tctccggcac ttgctcccct
    19321 gttaggaagc tggctgctgg tccatttttc ctggcaggcg attttcgcca ccctgtttgc
    19381 cattaccgtg gtgctgattc tgcctatttt ctggctcaaa cccacgacga aggcccgtaa
    19441 caatagtcag gatggtctga cctttaccga cctgctacgt tctaaaacct atcgcggcaa
    19501 cgtgctgata tatgcggcct gttcagccag tttttttgca tggctgaccg gttcaccgtt
    19561 catccttagt gaaatgggct acagcccggc agttattggt ttaagttatg tcccgcaaac
    19621 tatcgcgttt ctgattggtg gttatggctg tcgcgccgca ctgcagaaat ggcaaggcaa
    19681 gcagttatta ccgtggttgc tggtgctgtt tgctgtcagc gtcattgcga cctgggctgc
    19741 aggcttcatt agccatgtgt cgctggtcga aatcctgatc ccattctgtg tgatggcgat
    19801 tgccaatggc gcgatctacc ctattgttgt cgcccaggcg ctgcgtccct tcccacacgc
    19861 aactggtcgc gccgcagctt tgcagaacac tctacaactg ggtctgtgct tcctcgcaag
    19921 tctggtagtt tcctggctga tcagtatcag cacgccattg ctcaccacca ccagcgtgat
    19981 gttatcaaca gtagtgctgg tcgcgctggg ttacatgatg caacgttgtg aagaagttgg
    20041 ctgccagaat catggcaatg ccgaagtcgc tcatagcgaa tcacactgat ctatatcgat
    20101 atacttatac ttaggctgct aacaaaattt tgttgtatga ttgaaattag cggcctatac
    20161 taatttcgag ttgttaaagc tacgataaat attatgtttt tacggggaca ggatcgttcc
    20221 cgactcacta tggatggtca tttcggcaag ggttcctcct ttccctctgt tctacgtcgg
    20281 attatagact cgcggttttt tctgcgagat ttctcacaaa gcccaaaaag cgtctacgct
    20341 gttttaaggt tctgatcacc gaccagtgat ggagaaacta tgagttcatc gtgtatagaa
    20401 gaagtcagcg taccggatga caactggtac cgtatcgcca acgaattact tagccgtgcc
    20461 ggtatagcca ttaacggttc tgccccggcg gatattcgtg tgaaaaaccc cgattttttt
    20521 aaacgcgttc tgcaagaagg ctctttgggg ttaggcgaaa gttatatgga tggctggtgg
    20581 gaatgtgacc gactggatat gttttttagc aaagtcttac gcgcaggtct cgagaaccaa
    20641 ctcccccatc atttcaaaga cacgctgcgt attgccggcg ctcgtctctt caatctgcag
    20701 agtaaaaaac gtgcctggat agtcggcaaa gagcattacg atttgggtaa tgacttgttc
    20761 agccgcatgc ttgatccctt catgcaatat tcctgcgctt actggaaaga tgccgataat
    20821 ctggaatctg cccagcaggc gaagctcaaa atgatttgtg aaaaattgca gttaaaacca
    20881 gggatgcgcg tactggatat tggctgcggc tggggcggac tggcacacta catggcatct
    20941 aattatgacg taagcgtggt gggcgtcacc atttctgccg aacagcaaaa aatggctcag
    21001 gaacgctgtg aaggcctgga tgtcaccatt ttgctgcaag attatcgtga cctgaacgac
    21061 cagtttgatc gtattgtttc tgtggggatg ttcgagcacg tcggaccgaa aaattacgat
    21121 acctattttg cggtggtgga tcgtaatttg aaaccggaag gcatattcct gctccatact
    21181 atcggttcga aaaaaaccga tctgaatgtt gatccctgga ttaacaaata tatttttccg
    21241 aacggttgcc tgccctctgt acgccagatt gctcaatcca gcgaatccca ctttgtgatg
    21301 gaagactggc ataacttcgg tgctgattac gatactacgt tgatggcgtg gtatgaacga
    21361 ttcctcgccg catggccaga aattgcggat aactacagtg aacgctttaa acgaatgttt
    21421 acctattatc tgaatgcctg tgcaggtgct ttccgcgccc gtgatattca gctctggcag
    21481 gtcgtgttct cacgcggtgt tgaaaacggc cttcgagtgg ctcgctaaag gctattctat
    21541 cgccccctct ccgggggcga tttcagatca ggcttctgta cctggttgat tcatggcatt
    21601 ttctcgtgcc gccagcacac gttctaccgt atctaccact gcctgagttt gtggatcgat
    21661 ttcaatgttg acgcgtgcgc caagtttttt cttcccaaga gtcgtgcgtt ccagtgtttc
    21721 cggaattaaa tggacgcaaa aacgcgttgg cgtgacttcg ccgacggtca ggctaatacc
    21781 gtcgatgcca ataaatcctt tgtacagaat atatttcatc aactgactat cctggacttt
    21841 aaaccagatc tggcgattat tttccgaggt taatattttc gccacttcag cggtggtcat
    21901 aatatgacct gacattaagt gtccgccaat ttcatcactg aatttcgccg cacgctcaac
    21961 gtttacccaa tcccccactt ttaaatcgcc aagattggta atgcgtaacg tttctttcat
    22021 caggtcaaaa ctgacatggt tgccgttaat ttccgtcacg gtcaggcagc aaccgttatg
    22081 cgccacggac gcaccggttt ccaggccgtc cagcatgtgg tcgggtaact ccaccacatg
    22141 cgtacgaaaa tttggtttct cgtcaatcga caccagtttt gcggtgccct gtacaatccc
    22201 cgtaaacata cttacaactc ctgaaatcag ttaagacatt ctgttcagca caatagcagg
    22261 tggcaaacgc ccttaccagt gaaggggtaa gaatgactat tttttcactg gagaattaat
    22321 aaatcctcgc tacaatagac tgaatttccc ctgcttcttc tttttgctgc ccattcaggc
    22381 ggctttttag tctctcatat aactacaaat aaaaggtgtt cacgtgcaga agtatatcag
    22441 tgaagcgcgt ctgttattag cattagcaat cccggtgatt ctcgcgcaaa tcgcccaaac
    22501 tgcgatgggt tttgtcgata ccgtgatggc gggcggctat agtgccaccg acatggcggc
    22561 ggtcgctatc ggtacttcta tctggcttcc ggcgatcctc tttggtcacg gactgctgct
    22621 ggcattaacg ccggttatcg cgcaattaaa tggttctggt cgacgtgagc gcattgcgca
    22681 tcaggtgcga caaggtttct ggctggcagg ttttgtttcc gttctcatta tgctggtgct
    22741 gtggaatgca ggttacatta tccgctccat ggaaaacatc gatccggctc tggcggagaa
    22801 agccgtgggt tatctgcgtg cgttgctgtg gggcgcgccg ggatatctgt tcttccaggt
    22861 tgcccgtaac caatgtgaag gtctggcaaa aaccaagccg ggtatggtaa tgggctttat
    22921 cggcctgctg gtgaacattc cggtgaacta tatctttatt tatggtcatt tcggtatgcc
    22981 tgagctcggt ggcgttggtt gtggcgtggc tactgcggcg gtgtattggg tcatgttcct
    23041 tgccatggtt tcttacatta aacgcgcccg ctccatgcgc gatatccgta acgaaaaagg
    23101 caccgcgaaa cccgatcctg cggttatgaa acgactgatt caactcggtt tgccgattgc
    23161 gctggcactg ttctttgaag tgacactgtt tgccgtcgtg gctctgttag tgtctccgct
    23221 cggtattgtt gatgtcgcag gacaccagat tgccctgaac tttagttcac tgatgtttgt
    23281 gcttccaatg tcgctggcgg cagcggtaac tatccgcgta ggttatcgtc tgggtcaggg
    23341 ctcaacgctg gatgcgcaaa ccgctgcgcg gaccgggctt atggtgggtg tctgtatggc
    23401 aaccctgacg gccatattca cggtttcact gcgggagcaa atcgccctgt tgtacaacga
    23461 caatcccgag gttgtaacgc tggctgcgca tttgatgttg ctggcggcgg tatatcagat
    23521 ttctgactca atccaggtca ttggcagtgg gattttgcgt ggttataaag atacgcgttc
    23581 cattttctat atcaccttta cggcttactg ggtgctgggc ttgccaagcg gctatattct
    23641 ggcactgacc gatctggtcg ttgaacctat ggggccagca ggcttctgga taggctttat
    23701 tattggcctg acgtcggcag ccattatgat gatgctgcgt atgcggttcc tgcaacgtat
    23761 gccgtcagcc atcattctgc aacgagcgtc ccgctaataa agacaaggcg caaccttcac
    23821 gggttgcgcc tgtattttta cgcaggctgg agcgttgcgc caatcccgtc ttcgtctggc
    23881 tgtagtttca gtgcgttaca cagagtttgc cgactggcat ctgttatctc aacgtgtata
    23941 tcaccggtaa cttccccttt cacattgatg aagtcaggtg aaatttcttt cgcactgatt
    24001 tgcaggcaaa tcgctccctc tccgcgcagg cgtttcactt tcagttgcac atgcgttgcc
    24061 gcaccagtga tcagccacag ttttccgcca ttcagattga tgttaccaac actgaaagtg
    24121 acactgtcac cttttaccgt actgccgtcg agaatcaaca caccgccctg ctgtacagta
    24181 atcgttgatt gttgcagctc tgcgccataa ccaataatca ttgaagcgcc gtccagtaat
    24241 gcaacactcc cggtcagttc aaccggtgcg cgatcttcct ctacgacaaa attttcgtcc
    24301 gcgctggtgg gtgacaacag gatcactgtt ccaccttgta ttaccgcatt ttccagcgta
    24361 ccggcatgca cttcagcccg accacccaca ttcacggaca ggttttgcgt cttaccggaa
    24421 ctgtagagct gaaggccatc cgtccccaga cgataaatgg atccattctc aacaatagtg
    24481 tcagagtctt caccaccgag attattcatg gtgccaccag caagcaacgt atcactggct
    24541 ttcccggcca gcataaaaac gccgccctga ttaatctcac aattgctggc ttccccacct
    24601 ggatacacca acaattcacc accttcatct accacgaccg ctgaggttgt cccattaacc
    24661 aacaggccgc cctcttgatc gagaatgatt ttttccgcgc gatggccttc cagtacacgc
    24721 aggttaccgc cattttccag caacaaaccg caggcataac ctttatcaac gctgaattca
    24781 ccttcgggat ggcggccatt aaccgtagcg agcgtagaga gactaattgc gcctccctcg
    24841 gactgaacaa tatctgtggc aatggcaccg tcgctgaccg atagcgtgcc attgttaccg
    24901 tactgtgtgc atttggcgat cccaccggtt gccactttca accagccagc ccccgtgacc
    24961 acggtatttt cggctcttcc gccagcttca acaatcaact cgccttcagt aagttgcgtc
    25021 gcgccaatga cctcgccggt cttaacaatt tgcacattcc cgtcgctcat caagtttttc
    25081 gcatcagatc ccatgattta ttcctttgct gcatctgtgt gcctttattg ctacctaagt
    25141 gtaaaggcta cggaggattt atccacgaca gatttgagat ggtggcaaac aactctgttt
    25201 aaactctgat acacgaatta ttgggttgta tcagatgtaa atgcgatcct gaataaaaat
    25261 cacccttgca aatcaacaaa atatagccaa tttgcttaaa agctctgcaa acggtcgaaa
    25321 tagcgcagaa aattacgttt tgcctcttgc caccttccca ctctgccgct aatattcgtc
    25381 cccgttgtca cctacaacgt tgcgttcata gctcagttgg ttagagcacc accttgacat
    25441 ggtgggggtc gttggttcga gtccaattga acgcaccatc ctgcgtccgt agctcagttg
    25501 gttagagcac caccttgaca tggtgggggt cggtggttcg agtccactcg gacgcaccag
    25561 attttcttaa tctggtcttt tcctttttcc ctctgtttct tctctgtatc caatacgtta
    25621 aaagatttac actatcttcg tatgcgttat cagaaggaga atcgctatgg caactttgtt
    25681 acaacttcat tttgctttta atggcccatt tggcgacgca atggctgagc agcttaaacc
    25741 gcttgctgag tcgattaatc aggaacctgg ttttctgtgg aaggtatgga cagaaagtga
    25801 aaagaaccac gaagccggtg ggatctatct ttttactgat gaaaaaagcg cccttgccta
    25861 tctggaaaaa catactgccc gacttaaaaa tctcggcgtt gaggaagttg tcgccaaagt
    25921 tttcgatgtc aatgagccac tgagtcaaat caatcaggca aaactcgcct gacagaattt
    25981 aatcaagggc ggttagcgcc cttttcatcc ctgtctgaaa tttctcaaat tctaaaaatc
    26041 tcaaccaaac ttatctgata acactaaatt cgaaagaatg cgtacaggta agtaacaatg
    26101 aaaaaaattg ctattgtggg tgccgggcct acggggatct acaccttatt ctcgcttcta
    26161 cagcaacaaa ctccactttc tatttctatc ttcgagcagg ctgacgaggc cggtgtcggg
    26221 atgccataca gtgatgagga aaactcaaaa atgatgctgg caaatattgc cagtattgaa
    26281 ataccgccga tttattgtac gtatctcgaa tggctacaaa agcaagaagc cagccatctc
    26341 cagcgttatg gcgttaaaaa agaaaccttg cacgatcgtc agtttttacc gcgaattctg
    26401 ctgggcgaat atttccgcga tcaattttta cgattagtag accaggcacg aaagcaaaaa
    26461 tttgcagtgg ctgtttatga atcatgccag gttaccgatc tgcaaattac aaatgctggc
    26521 gtcatgctcg ctacaaatca ggatttaccc agcgagacgt ttgatttagc ggtgattgcc
    26581 acgggtcacg tctggcctga tgaagaagaa gcaacccgaa cgtattttcc cagcccgtgg
    26641 tcaggcctga tggaagcaaa ggtcgatgcg tgtaacgtgg gtattatggg aacatccttg
    26701 agcggactgg atgcggcaat ggcagtggct attcagcatg gttcgttcat tgaagatgat
    26761 aaacaacacg tcgtttttca ccgcgataac gcaagtgaaa agctaaatat cacgttgatg
    26821 tcgcgcacgg gtattttacc cgaagccgat ttctattgcc ctattcccta cgagccctta
    26881 cacatcgtca ccgatcaggc attaaatgct gagattcaaa aaggcgaaga gggccttttg
    26941 gatcgggtat ttagattgat agtagaggaa atcaagtttg ctgatccaga ctggagtcaa
    27001 cgcatagcct tagagagcct gaatgtcgat tcctttgctc aagcctggtt tgccgagcgc
    27061 aaacaacgcg acccatttga ctgggcagaa aaaaatctcc aggaagtcga acgcaataaa
    27121 cgagaaaaac atactgttcc ctggcgttat gtcattctgc gcctgcatga agccgtacag
    27181 gaaattgttc cacatctgaa tgaacacgac cataaacggt tcagtaaagg ccttgcccgg
    27241 gttttcatcg ataattatgc ggcaatccct tcagagtcta ttcgtcgcct acttgcctta
    27301 cgtgaagcgg gaatcattca tattctcgcc ctcggtgaag actacaaaat ggaaattaat
    27361 gagtcgcgca ccgtcctgaa aacggaagac aacagctact cgtttgacgt ttttatcgat
    27421 gcccgcgggc agcgtccgct taaagtgaaa gatattcctt tccatggact acgcgaacaa
    27481 ttacagaaaa caggggatga aatccctgat gttggtgaag attatacgtt acagcaaccc
    27541 gaagatattc gtgggcgcgt agcgttcggc gcgttgccct ggttaatgca cgaccagcct
    27601 ttcgttcagg gacttacggc atgtgcagaa attggtgagg cgatggctcg ggcggtcgta
    27661 aaacctgcct cccgtgcacg tcggcgtctt tcgtttgatt aaaggtaaag ctatcagcac
    27721 tcaacaaact gatgctgttc attaagacga tagcggactc cggctcgaat attgttttca
    27781 ccttcaatgg cgacggcgaa gcgaactctt tcgccgtcat gatataccag tgcggcgctt
    27841 cctcctggcc cgagaataat tgagtcgacc acgcccgtgc tcactaccgt tgaattttca
    27901 ccgctggcaa tgatgtgtac attgtcgcca ctgttggcaa ttctggcgtt tgcgccaaag
    27961 ctggcgattt gcaccaggtc accattactg gcgacatgac atcgttcacc caatgtacaa
    28021 acgcgtactc gcattccggt attcgcaatc cgactgctat ctccagcgct gcttatccgc
    28081 gaagaattac cggttacagc aacccgtgcg cgttcaccgg agctgccaat gtgactgtta
    28141 aagccgacac ttccaatccg cacgctataa cccatactgg caatttgcgc ggcataccca
    28201 gcggttgcga ttctggcgtt atcctcacaa cagccgatgc gagcatgatc gtcgctgcgg
    28261 gcaaactgac tgcaaaatgg tctctctccg gggtctgtgg ccagtttttg catagaggaa
    28321 acttcctgat gcgcgaagtt ttcatccgcc agccattttt tccacgcata ctcaaccaga
    28381 ctctccgccc agtccgtata tccttcctca atcagtgcat gatgaatatc ggcataacta
    28441 ccgccgcgtg ggaaatggcg caggaaccag cgatacatca ccgctccgat acgccattct
    28501 cgcaggtctg cttgtgtaat gatcattcgt tagtgccggt gatagccatc gaccatgctt
    28561 ttaaaggttt cgtgtggcgt acgccccgtg gtgcaaagat aaagatgacc gaagataaaa
    28621 aagaggctta taaatgccag agcaaaatgt gtctgcaata accagtatct tacgccagga
    28681 aacacatctc ccacggcttg cggatagaga cacagcagcc ccgtcaatag taacaacggc
    28741 agcaatccat acatgacacc aacataggca acctgctgta agggattaaa tttagactgg
    28801 gttgttgccg ggaaaggatg ttcttccccc tgcataatgc caaacaaata aaatcgcgtt
    28861 tgttttgccg cccgctccag ccaaccctga cgacgaatgc gatagtggtg gccattaccc
    28921 ccaacggcat tgatcagaac aaagccgagc cagcatgcca gtaacaaaaa tccgcaaact
    28981 tcatgcaccg caaccagact tttaaccgca gttgcgccca ccagcgcaaa gtgatttatc
    29041 agcccacttg ccagcaacaa tacaaagagt aacgcattcg accagtgcca taatctgacc
    29101 gctttgctgt aaagataaat cttctcacca tgatcggtgg ctgatttctt cacgccacga
    29161 gcacgaagca aggcgtgcag ccccagcacc aaccacatcc caaccagcaa tactccggca
    29221 atgatcaacc acaccggcca aaattcggga gtgaactgtg gcacatagtt cgctaactgg
    29281 ctctgaaact gttcagcatg ttgcgacggg ttcatacatt ttccttttta atcaaatgtt
    29341 gaccgaaccg gcgatacaga tgcggtttcc cggcaccagg tagctgatat tgatagtatt
    29401 tattatcctg caaccacgcc tgaatttctg ggctatcttc gcgaccaaag atcaatgcgt
    29461 gttccggaca ggcgctgacg caaatgggcg gaaagccttt tgccagtcgg gactcagcgc
    29521 agaaatcaca tttatctgcc actttagtga cagggttaag gtagcgaacc tggtacggac
    29581 aagcgccaat gcaataactg cagccgatgc actgcgattt ttctacccgc acgatcccct
    29641 gctcatcacg ccacgatgcc cccgtcgggc atacgtcgat gcacggagca tcttcgcagt
    29701 gctgacatga ctggcgaaaa aagtgatact gcgtttcgtt gtcgttatcg gtgactggaa
    29761 tgtgtgctat cgataaacgg cttccctgag cggggacgtg gttagtttta cggcatgcgc
    29821 gggcgcaaat attacagcca ttgcaccgtg actcgtcatg aatcatggca taccgaactt
    29881 ccttttcttg cctcgtgttc gctaacagag aagacgcaga accagtaaaa aagattactg
    29941 ttcccatccc cagaacaaat ttgcgtcgag tgaaggacat ctgattatcc cctaatgcag
    30001 tggctgcaac tgtccaccct atccatgaca tctccctgaa atttcagggg ttctgaaact
    30061 gctctacctc gaaccattcg cataagccat aagccttgcc ataggtcata aatcgctcgc
    30121 taaacaagta caacaccgcc gtcgatgact ccacctgccg tatatcggca tattcaggtg
    30181 caacatccat cgcggcgatc gcggcttcaa tctgcgcttc ctggaagtaa tatggcgctt
    30241 gcatcagcat ggcgactttg taaggacgag gatacgtctg acactcaaaa cgaacggcgt
    30301 gtgcaatggc gcgacaaatg tcctgctcca caacttgcaa agacatcgcc gcgtagtttt
    30361 cactcatggc ctgcgttgag taatagtaat catcctgact tcccgtcata caagcgatat
    30421 ccgcgtagtc tccgttctgc ttaatgcttt ccagcaaaac tgacagttcc tcttctgcaa
    30481 ccgaataggg tggcgagaga aacacggcgc gcgccaccag ctgcccagcc gcagaatgct
    30541 ggcgaatgaa ctctgccagg atttcagccg gttctggttc ggttaccgta tcaacggggt
    30601 gacgcatccc ctgtaaccat tgccgcttcg cctcgtcaac ttcgctgact ttcgctaacg
    30661 gcaattcatc ccgatggttc atctcaccca ttccttacac aggcagtaga ttgtgtgctg
    30721 ccagatcggc ggcaatatct tccagcccca gacgctgcaa tgtttcgcgg gtcggacaac
    30781 caagctgtgg gtcccagccc atttctttgt agaacatcgt gagcgaagca tgcatatcgt
    30841 cacgatccat tttgtccgta ccttcagtaa agaccgggat ctgcggatcc ttgtcgaata
    30901 cccaggaaca gataagatcg tgttcattac gcatatcttt ggtttgcatc agttttaccg
    30961 tgtaggcacg atgcaacgta aagatacgct ctgcagctaa atctaatttt tcctgagtca
    31021 tctcttcgcc ggtgatcgct ttgaagaatt tggcttcaag cgccagatcg ccccggtaat
    31081 tacggctttt cagcggcgaa acggtcattg gccagaccca gttacacagg gtgacggcgt
    31141 tatgcaaaca gacccgcaac agcgaccact tagcatattt aatttttgcg tcattgattg
    31201 gcgtgtagtt tttggtttca tcgtaggcat cttcagaacc aaaaagctct ttcgccactt
    31261 cacgttgcag tttcaatggc aagccggaac caataaagtt gatatgggta tgtgtcatgc
    31321 agtcacggtt gaacatacag ttaacaatgg aaccgacctg cgctgacgct tcattggcat
    31381 ggtgaaccgg atagccaaac ggcgaccaga gtttattttt cgcgtagccc cagtactctt
    31441 cacccaaatt ccagcgttct gcgatggcat atgaaccatc agccaggtga ctcagctcac
    31501 ccacacgatg cgccagacgg tagtaaaaat ctttaatgaa attaacgtca cccgcttcca
    31561 gttgatccca gtgaatttcc gcatactctt cagcaggcag aacacgcttg aacacccctt
    31621 tgctgtaaca ataagtaaag tcgcgatgca actgcccgta gttacaccat aggccgtagt
    31681 catcgaacaa attcagacca accaagttac caatcacacg gccatcgtct ttatcttcaa
    31741 aatctttcgg ccggttcggg aagatggtgg tatggacaaa gtttgctaca caagtgttac
    31801 cacctgtgct gggcacgcca aactctttca cccgaggaat attcatttgg gtcatacaac
    31861 ggatcgggca agagtggcag ccgctcattt ttactgtgta tttctctgcc gccggtccta
    31921 agtcaaaaac ggatttatag gtacgaaagc cgaccgtatt ctgattgcct ggcggaattt
    31981 cacccgtttc aatcggacca ccttcagccg cgccccaaaa cagcccttta cgtgctgtcc
    32041 agcgtgactt gggatctgaa tactctgccc acgattgtgg cgtacttggc acgacatggt
    32101 tgttattcgc accaataagt tcagtcatca tgtaatcatt gagacgcttc atctcctgac
    32161 gatcggcaat gttgacccct ttcgtccctt caaccgcaat cgctttcagg tttttcgaac
    32221 ccattattgc gccagttccc gcaccgccgc tgtggttacg gctattcaac atgccagaga
    32281 gaggaacaag gttttcccca gcctgaccaa tagccgccac acaggtttcc ggactggtca
    32341 atcgacaaat ttcttccgtc gttgcgcgcg tcccttttcc ccataagaaa tcggcttttt
    32401 ccaggctaac tttgtcatct ttaatcttca gccataccgg tgatttcgcc ttcccttcga
    32461 taataatgac gtcgtatcca gcgaatttca tttgcgctgc aaaaaagcca cccatatggg
    32521 catcgacgac taaatttcct ttggtaaaag tagaaagtga ggtgatattt acgcgagaac
    32581 tacagggggc accagatcca gttaatgggc cggtagcaaa gactaattta ttcgcttcat
    32641 cgaaaggttt cgtgcctggc ggtacttcgt catacataat tttgtagccg aagcccatgc
    32701 caccgacaaa acttttaaac ttactggaat cttcgagggt aatatttcct gtcgtgagat
    32761 tgactcttaa tatattacct gtccaaccgt tagccatgat tttttccttt gcaagattca
    32821 cacagtaata tctttccact cgataatttt taacgcccct gttgggcagg cgtttgcgca
    32881 ttcaccgcat aacacacatt tcgaggattt tttactttcg gtatttacgg tggccatcat
    32941 ccacgggcat gccgtagtac aggcgctaca gccaatacag cgtttatgat cgacggtaat
    33001 acagccttct ttctgctgcc aggtaatcgc accaatcgga cagacgttca tgcattgcgg
    33061 ctctttgcat tgacggcagg tgtccgcggt atagttgaga tcgccataca ggccgccgcc
    33121 agagccaacc ccgttgtcgc caaagaaata attgcgatgg attttgatac gggagaagaa
    33181 tgtccctact gagccatcgt tgaagttggt acaagagatt tcacagcggt ggcaacccgt
    33241 gcatcgcgct cgctgggtca ccagcacccc tttgggtgta tttatcaacc ctactgtgcc
    33301 gctatcaata tcttcctgtt tgcaacccaa aagtgacagt aacgcagggg caattgttag
    33361 accagcaagg ccttttcctg atatacgcaa gaattctaat cgcgttaagc caatatctaa
    33421 tagtggacga tcaaccgggt tcatttattt cgcatcctct tcgcaaacac agtgctgaca
    33481 attcagcagc ctgatattaa gcattcaact aatacgctgc tgactctgtc cccaacacca
    33541 gatattttcc ctaacccgat agtgtatgag aaaagattta acgccgataa actaatcctt
    33601 tatggttagg agaaa
//
LOCUS       DAPHBJ010000029        32753 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.029, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000029 DAPHBJ010000000
VERSION     DAPHBJ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.029"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..89
                     /locus_tag="RHZ76_001274"
     CDS             <1..89
                     /locus_tag="RHZ76_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HDV2811941.1"
                     /translation="LHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            103..1395
                     /locus_tag="RHZ76_001275"
     CDS             103..1395
                     /locus_tag="RHZ76_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV2811942.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPENVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            1416..2251
                     /locus_tag="RHZ76_001276"
                     /pseudo
     CDS             1416..2251
                     /locus_tag="RHZ76_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            2238..3080
                     /locus_tag="RHZ76_001277"
     CDS             2238..3080
                     /locus_tag="RHZ76_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDV2811943.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            3111..4163
                     /locus_tag="RHZ76_001278"
     CDS             3111..4163
                     /locus_tag="RHZ76_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDV2811944.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            4181..4969
                     /locus_tag="RHZ76_001279"
     CDS             4181..4969
                     /locus_tag="RHZ76_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDV2811945.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            4979..6034
                     /locus_tag="RHZ76_001280"
     CDS             4979..6034
                     /locus_tag="RHZ76_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV2811946.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPGQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            6031..8298
                     /gene="ycjT"
                     /locus_tag="RHZ76_001281"
     CDS             6031..8298
                     /gene="ycjT"
                     /locus_tag="RHZ76_001281"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HDV2811947.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSHEAWQRELDFASGELR
                     RNVVWRTSNGSGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGSYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETMTLQKLVWIDWRDDRQAALDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAYTSYMARYNVQQALNIARQFGCSDDA
                     FIHRAEMFLKELWMPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLITVAEESVFCLGDF
                     ILPFNGTATKHQEDE"
     gene            8295..8954
                     /gene="pgmB"
                     /locus_tag="RHZ76_001282"
     CDS             8295..8954
                     /gene="pgmB"
                     /locus_tag="RHZ76_001282"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDV2811948.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTESLTWPRLSAFWQN
                     V"
     gene            8968..10050
                     /locus_tag="RHZ76_001283"
     CDS             8968..10050
                     /locus_tag="RHZ76_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2811949.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVSGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            10095..11000
                     /gene="ompG"
                     /locus_tag="RHZ76_001284"
     CDS             10095..11000
                     /gene="ompG"
                     /locus_tag="RHZ76_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HDV2811950.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(11111..12109)
                     /locus_tag="RHZ76_001285"
     CDS             complement(11111..12109)
                     /locus_tag="RHZ76_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV2811951.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALKAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            12265..13662
                     /locus_tag="RHZ76_001286"
     CDS             12265..13662
                     /locus_tag="RHZ76_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HDV2811952.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMSEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            13659..14720
                     /locus_tag="RHZ76_001287"
     CDS             13659..14720
                     /locus_tag="RHZ76_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HDV2811953.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            14868..16409
                     /gene="tyrR"
                     /locus_tag="RHZ76_001288"
     CDS             14868..16409
                     /gene="tyrR"
                     /locus_tag="RHZ76_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HDV2811954.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(16453..16959)
                     /gene="tpx"
                     /locus_tag="RHZ76_001289"
     CDS             complement(16453..16959)
                     /gene="tpx"
                     /locus_tag="RHZ76_001289"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDV2811955.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            17078..18043
                     /gene="ycjG"
                     /locus_tag="RHZ76_001290"
     CDS             17078..18043
                     /gene="ycjG"
                     /locus_tag="RHZ76_001290"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HDV2811956.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARR
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(18018..18746)
                     /gene="mpaA"
                     /locus_tag="RHZ76_001291"
     CDS             complement(18018..18746)
                     /gene="mpaA"
                     /locus_tag="RHZ76_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HDV2811957.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(18873..19010)
                     /locus_tag="RHZ76_001292"
                     /pseudo
     CDS             complement(18873..19010)
                     /locus_tag="RHZ76_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
     gene            complement(19081..20001)
                     /locus_tag="RHZ76_001293"
     CDS             complement(19081..20001)
                     /locus_tag="RHZ76_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV2811958.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVLSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSIFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            20139..21038
                     /gene="pgrR"
                     /locus_tag="RHZ76_001294"
     CDS             20139..21038
                     /gene="pgrR"
                     /locus_tag="RHZ76_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HDV2811959.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSAMASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            21375..22988
                     /gene="mppA"
                     /locus_tag="RHZ76_001295"
     CDS             21375..22988
                     /gene="mppA"
                     /locus_tag="RHZ76_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HDV2811960.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(23039..24070)
                     /gene="ynaI"
                     /locus_tag="RHZ76_001296"
     CDS             complement(23039..24070)
                     /gene="ynaI"
                     /locus_tag="RHZ76_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HDV2811961.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            24314..24571
                     /locus_tag="RHZ76_001297"
     CDS             24314..24571
                     /locus_tag="RHZ76_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HDV2811962.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(24621..25571)
                     /gene="uspE"
                     /locus_tag="RHZ76_001298"
     CDS             complement(24621..25571)
                     /gene="uspE"
                     /locus_tag="RHZ76_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HDV2811963.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(25723..26475)
                     /gene="fnr"
                     /locus_tag="RHZ76_001299"
     CDS             complement(25723..26475)
                     /gene="fnr"
                     /locus_tag="RHZ76_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HDV2811964.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(26670..27185)
                     /gene="ogt"
                     /locus_tag="RHZ76_001300"
     CDS             complement(26670..27185)
                     /gene="ogt"
                     /locus_tag="RHZ76_001300"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDV2811965.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(27196..28721)
                     /gene="abgT"
                     /locus_tag="RHZ76_001301"
                     /pseudo
     CDS             complement(27196..28721)
                     /gene="abgT"
                     /locus_tag="RHZ76_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
     gene            complement(28758..30203)
                     /gene="abgB"
                     /locus_tag="RHZ76_001302"
     CDS             complement(28758..30203)
                     /gene="abgB"
                     /locus_tag="RHZ76_001302"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HDV2811966.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRCALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQ
                     ATLTSNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFLDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            complement(30203..31513)
                     /gene="abgA"
                     /locus_tag="RHZ76_001303"
     CDS             complement(30203..31513)
                     /gene="abgA"
                     /locus_tag="RHZ76_001303"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HDV2811967.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELH
                     QLGYSLALGREVVNESSRMGLPDEFTLQREFERARQQGALAQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            31689..32597
                     /locus_tag="RHZ76_001304"
     CDS             31689..32597
                     /locus_tag="RHZ76_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2811968.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
BASE COUNT         8119 a         7946 c         8643 g         8045 t
ORIGIN      
        1 atctgcatgg tcgggatctg attagtgaag ttgatatatt gggtaatgaa ataacgctgc
       61 gcccctggca ggttatgtgg attaaataaa aaggaacatc tcatgattaa atcaaaaatc
      121 gtgctgttat cagcactggt ttcctgcgcc ctgatttcag gctgtaaaga agaaaataaa
      181 acgaatgtat ccatcgaatt tatgcattct tcggtggagc aggagcgtca ggccgttatc
      241 agtaaattga ttgcccgttt tgaaaaagaa aaccctggca tcaccgttaa gcaagtgccc
      301 gtggaagaag atgcctataa cactaaagtc attactcttt cacgtagcgg ttcgctgccg
      361 gaagtgatcg aaaccagcca tgactacgcc aaagtgatgg acaaagagca gcttatcgat
      421 cgcaaagcgg ttgccacagt catcagcaac gttggtgaag gcgcgtttta cgatggcgta
      481 ctgcgtattg tgcgtaccga agatggtagc gcatggaccg gtgttcctgt cagcgcctgg
      541 attggcggta tctggtatcg caaagatgtg ctggcaaaag cggggcttga ggagccgaaa
      601 aactggcaac agctgctgga cgttgcacag aaactgaatg acccggcgaa taaaaaatac
      661 ggcattgcgc tgcctacagc agaaagcgtg ttgacggaac aatccttctc ccagtttgcg
      721 ttatccaacc aggctaacgt ctttaacgcc gaaggcaaaa tcacccttga tacaccagag
      781 atgatgcagg cactgaccta ttaccgcgac cttgctgcca acactatgcc gggttctaac
      841 gacatcatgg aagtgaaaga cgcctttatg aacggcaccg cgccgatggc gatttactcc
      901 acctatatcc ttccggctgt gattaaagaa ggcgacccgg aaaacgtcgg tttcgtggtg
      961 ccaaccgaga aaaactctgc ggtctacggc atgttgacct cgctgaccat taccgccggg
     1021 caaaagaccg aagagacgga agcagcagaa aaatttgtca cctttatgga gcaggcagac
     1081 aacattgccg actgggtgat gatgtcgcca ggtgctgcgc tgccggtgaa taaagcggtt
     1141 gtcactaccg ccacctggaa agacaacgac gttattaagg cgctgggtga actaccgaat
     1201 cagctaatcg gtgaactgcc aaatattcag gtctttggcg cagtagggga taaaaacttt
     1261 acccgcatgg gtgatgtgac gggttctggc gtggtgagct ccatggtgca taacgtcacc
     1321 gtgggtaaag ccgatctccc tggcacgctg caagcgagcc agaaaaagct ggatgaactg
     1381 atcgaacagc actaaaccca ggacaggaat ccgcaatgaa caggcttttt tcaggtcgtt
     1441 ccgatatgcc ctttgcgctg ctgcttctcg cgcccagctt attactgctg ggcggtctgg
     1501 tggcgtggcc gatggtgtcg aatatcgaaa tcagtttttt acgtctgccg ctcaatccca
     1561 acatcgagtc aacgtttgtt ggggtgagca actatgtgcg tatcctctcc gatcccggct
     1621 tctggcattc gctgtggatg acggtctggt ataccgcgct ggtggtggcg ggcagcaccg
     1681 tgctcgggct ggcggtggcg atgtttttca accgcgagtt ccgcctgcgc aaaaccgcgc
     1741 gttcgctggt gatcctctcc tacgtaacgc cgtccatttc gctggtgttc gcctggaaat
     1801 acatgttcaa caacggctac ggcattgtta actacctcgg cgtcgatctt ctgcatctct
     1861 atgagcaggc accgctgtgg ttcgataatc cgggcagtag ctttgtgctg gtggtgctgt
     1921 tcgccatctg gcgctacttc ccgtatgcct ttatctcgtt tctggcgatt ttgcagacca
     1981 tcgacaaatc gctgtacgaa gcggcagaga tggatggcgc taatgcctgg caacggtggt
     2041 gacactgcgc accatctgga tgttctacat gttcgcggat gtttatttgc tgacgaccaa
     2101 ggtcgatatt ctcggtgtat atctctacaa aaccgccttt gcctttaatg acttagggaa
     2161 agcggcggcg atctcggtgg tgctcttcat cattattttc gctgtcattc tgctgaccag
     2221 gaaacgggtg aacctcaatg gcaacaaata aacgcacact cagtcgaatc ggtttttact
     2281 gcgggctggc gctgtttctc atcatcacgc tgtttccgtt ttttgtgatg ctgatgacct
     2341 cgttcaagag cgcaaaagag gcgatctcgc tgcatcctac gctgctgccg caacagtgga
     2401 cgctggagca ttatgtcgac atttttaacc cggtgatttt cccgtttgtc gactacttcc
     2461 gtaacagcat ggtggtgtcg gtggtttcat ccgtggtggc ggtatttctc ggcattctcg
     2521 gggcttacgc gctttctcgc ctgcgcttta aaggccggat gaccatcaac gccagctttt
     2581 acacggtgta tatgttctct ggcattttgc tggtggtgcc gctgttcaaa atcatcaccg
     2641 cgcttggtat ttacgacacc gagatggcgc tgatcattac catggtgacg caaaccctgc
     2701 ccaccgccgt gtttatgctg aaaagctact tcgacaccat ccctgatgag atcgaagaag
     2761 cggcgatgat ggatggcctc aaccgtctgc aaattatctt ccgtatcacc gtgcccctgg
     2821 cgatgtccgg gctgatatcg gtgttcgtct actgctttat ggtggcatgg aacgactatc
     2881 tgtttgcgtc gattttcctc tccagcgcca gcaatttcac cttaccagtc ggcctgaacg
     2941 cgctgtttag tacgcctgat tacatctggg gacggatgat ggcggcctcg ctggtgaccg
     3001 cattgccggt ggtgattatg tatgcgcttt cggaacgttt tattaagagt ggtttgaccg
     3061 ccggtggcgt gaagggctaa agcggccagt ttctttacaa ggagttttac atgaaaaagt
     3121 tagtagccac agcaccgcgt gttgctgcgc tggttgagta tgaagatcgg gcgattttag
     3181 ccaatgaagt gaagatccgc gtgcgtttcg gcgcaccgaa acacggaacg gaagtggtcg
     3241 acttccgtgc cgccagcccg tttatcgatg aagactttaa cggcgagtgg cagatgttca
     3301 ccccgcgtcc ggcagatgcg ccgcgcggca ttgagtttgg caaattccag cttggcaaca
     3361 tggtggttgg cgacattatc gagtgcggca gcgacgttac agactacgcg gtgggcgaca
     3421 gcgtatgcgg ctacggcccg ctctccgaga cggtcatcat taacgcagtg aataactaca
     3481 agctgcgcaa aatgccgcaa ggcagctcct ggaaaaacgc cgtctgttac gacccggcgc
     3541 agtttgccat gagcggcgtg cgcgatgcca acgtacgcgt aggggatttt gtcgtggtgg
     3601 tagggcttgg cgcgatcggt caaattgcca tccaactggc gaaacgcgct ggcgcatcgg
     3661 tggtaattgg cgtcgatcct atcgcccatc gctgtgatat tgcccgtcgc cacggtgcgg
     3721 atttctgcct taaccccatt ggcactgacg taggcaaaga gatcaaaacg ctgaccggca
     3781 agcagggtgc cgatgtgatt atcgaaacca gcggttacgc cgacgcgcta caatcggcgc
     3841 tgcgcggtct ggcctacggc ggcaccatct cctatgtcgc gtttgccaaa ccatttgctg
     3901 aaggttttaa cctcggacgc gaagcgcatt tcaataacgc caaaattgtt ttctcacgcg
     3961 cgtgcagcga accgaacccg gattatccgc gctggagccg taagcgtatt gaagaaacct
     4021 gctgggaact gctgatgaac ggttatctca attgcgaaga tttaatcgac ccggtagtga
     4081 cctttgccaa cagcccggaa agctacatgc agtatgtcga tcagcatccg gaacagagca
     4141 tcaaaatggg cgttacgttt taattcaaag gaatgaaaaa atgaaaatag gcacacagaa
     4201 tcaggcgttc ttcccggaaa atattctgga gaaatttcgt tatatcaaag agatgggttt
     4261 cgatggtttt gagattgacg gcaaactgct ggtcaacaac atcgaagaag tcaaagcggc
     4321 gatcaaagaa accggtttac cggtgaccac cgcctgcggt ggctatgacg gctggattgg
     4381 tgactttatc gaagagcgtc gtcttaatgg cttaaagcag atcgaacgca ttctcgaagc
     4441 gctggcagaa gtgggcggta aaggtatcgt cgttccggca gcgtggggca tgtttacctt
     4501 ccgcttaccg ccgatgactt caccgcgtag tctggacggc gaccgcaaaa tggtgagtga
     4561 ttccctacgc gtactggagc aggtcgccgc gcgtaccgga accgtggtgt atctcgaacc
     4621 gttaaaccgc tatcaggatc atatgatcaa caccctcgcc gatgctcgtc gttacatcgt
     4681 cgaaaacaat cttaaacatg tgcagattat cggcgatttc tatcacatga acatcgaaga
     4741 agataacctg gcgcaggcgc tgcacgacaa ccgtgacttg ctcggtcatg tgcatattgc
     4801 cgataaccat cgctaccagc cgggcagcgg caccctcgat ttccacgcgc tgtttgaaca
     4861 gctgcgcgcc gataactatc agggttatgt ggtgtatgaa gggcgtatcc gggcagaaga
     4921 tcctgcccag gcgtaccgtg attcgttggc ctggttgcgt acctgctaag aggtctttgt
     4981 gaaaagtgca atgacaagct ctccgctgcg ggtcgcgata ataggcgcag gccaggttgc
     5041 ggataaggtc catgcttcgt actactgcac ccgcaacgat ctggaactgg tggctgtctg
     5101 tgacagccgc ctttcccagg cgcaggcgct ggcagaaaaa tacgggaatg catccgtgtg
     5161 ggacgatccg caggccatgc tgctggcggt gaaacctgac gtggttagcg tctgctcacc
     5221 taatcgtttt cattacgaac ataccctgat ggcgcttgaa gcgggctgcc atgtgatgtg
     5281 cgaaaaaccg cccgccatga cgccaggtca ggcgcgggaa atgtgcgata ccgcgcgcaa
     5341 actgggcaag gtgctggcct acgactttca ccatcgtttt gcgctcgata cgcaacagct
     5401 gcgtgaacag gtgaccaacg gcgttttggg agagatttac gttaccaccg cccgcgccct
     5461 gcgtcgctgc ggcgttcccg gctggggtgt ctttaccaat aaagaactgc agggtggtgg
     5521 cccgctgatc gacatcggca ttcatatgct ggatgctgcg atgtatgtgc tgggctttcc
     5581 ggcggtgaaa agcgtgaatg cgcatagctt tcaaaagatc ggcacgcaaa agagctgtgg
     5641 tcaatttggt gagtgggatc cggcaactta cagcgtcgaa gattcgctgt ttggcaccat
     5701 tgaatttcat aacggcggca ttctgtggct ggaaacgtca tttgcactca acatccgcga
     5761 acagtcgatt atgaacgtca gcttttgtgg tgataaagct ggtgcgacgc tgtttccagc
     5821 acatatctac accgataaca acggtgaatt aatgacgctg atgcaacggg aaatggcaga
     5881 cgacaaccgc catttgcgca gcatggaagc ctttatcaat cacgtacagg gcaagcccgt
     5941 gatgatagcc gacgccgagc aggggtacat catccagcaa ctggtggcgg cgttgtatca
     6001 atccgcagaa acagggacgc gtgtggaatt atgaccaggc cagtaacgtt atcagaaccc
     6061 catttcagcc agcataccct gaacaagtat gcatcgctga tggcgcaggg gaacggctat
     6121 cttgggcttc gcgccagcca tgaagaagat tacacgcgcc agacgcgagg gatgtatctg
     6181 gcggggctgt atcatcgggc gggaaaaggt gaaatcaacg aactggtgaa cctgcctgat
     6241 gtcgtgggga tggagattgc cataaatggt gaggttttct cgttatccca cgaagcctgg
     6301 cagcgtgagc ttgacttcgc cagtggcgaa ttacgccgca atgttgtctg gcgtaccagc
     6361 aacggctcag gttacaccat cgccagccgt cgctttgttt cggcagacca actgccgctc
     6421 attgcgctgg aaatcactat tacgccactg gacgccgacg cgtcagtgct gatttcaaca
     6481 ggcattgacg ccacgcaaac caatcacggt cgccaacatc tcgacgaaac ccaggtgcgg
     6541 gtgtttggtc agcatctgat gcaggggagc tacaccaccc aggatggacg cagtgatgtg
     6601 gccatcagct gttgctgtaa ggtgagcggt gatgtgcagc aatgctatac cgccaaagag
     6661 cgccgtttac tgcaacatac cagtgcgcag cttcatgcag gcgagacaat gacgttgcaa
     6721 aaactggtgt ggatcgactg gcgggatgac aggcaagctg ctttagacga gtggggcagc
     6781 gcgtcgcttc gccagcttga aatgtgcgcg cagcagagtt acgaccaact tcttgcagca
     6841 tcaacagaaa actggcgtca atggtggcag aaacgtcgta tcacggtaaa tggcggcgaa
     6901 gcgcacgatc agcaagcgtt agattatgcg ctttatcatc tgcgcatcat gacgcctgcc
     6961 cacgacgagc gcagcagcat tgcggcaaaa ggcttaaccg gcgaaggcta caaaggccac
     7021 gttttctggg atacagaagt atttttgtta ccgtttcatc tgtttagcga tccgacggtt
     7081 gcccgaagtt tactgcgtta tcgctggcac aacttgccag gcgcgcagga gaaagcgcga
     7141 cgcaacggct ggcagggcgc gctatttccg tgggaaagcg cgcgcagcgg cgaagaagag
     7201 acgccggaat ttgccgccat taacattcgc accgggctgc ggcaaaaagt ggcctcggcg
     7261 caggcggaac atcatctggt ggccgatatc gcctgggcgg ttattcaata ctggcagacc
     7321 acgggggatg aaagtttcat tgcgcatgaa ggcatggcgc tacttctgga gacggcaaag
     7381 ttctggatta gccgcgcggt gagagttaac gatcgtctgg aaattcatga tgttattggg
     7441 ccagacgaat ataccgaaca tgtcaataat aatgcataca ccagctatat ggcccgctac
     7501 aacgttcaac aggcgctgaa tattgcccgc cagttcggct gtagcgacga tgcgtttatc
     7561 catcgcgccg aaatgttcct caaagagcta tggatgccag aaattcagcc cgacggcgtt
     7621 ttgccgcagg atgattcgtt tatggctaag ccggcgatta atctggcgaa atacaaagcg
     7681 gcggcgggga agcaaaccat actgctggat tattcacgcg cagaagtgaa cgagatgcag
     7741 atcctcaaac aagctgatgt ggtgatgctc aattacatgc tgccggagca gttctcagcg
     7801 gcatcgtgtc ttgccaatct gcaattttat gaaccgcgca ctattcacga ctcgtcatta
     7861 agtaaagcaa tccacggcat tgttgccgca cgctgtggcc tgctgaccca aagttatcag
     7921 ttctggcgcg aggggactga aatcgatctt ggtgctgatc cgcatagttg tgatgatggt
     7981 atccatgctg ccgcaactgg cgctatctgg ctgggggcga ttcagggttt tgccggggtg
     8041 agcgtgcgtg acggtgaatt gcatctcaat ccggcgttac ctgagcagtg gcaacagttg
     8101 tctttccctc tgttctggca gggctgcgaa ttacaggtca ctcttgacgc gcagcgtatt
     8161 gcgattcgaa cttctgcgcc cgtttcactg cgtttgaacg gtcagcttat aaccgtggct
     8221 gaagaatctg ttttctgttt gggtgatttt attttgccct tcaatgggac cgctaccaaa
     8281 catcaggagg atgaatgaaa ctgcaagggg taattttcga tctggatggt gtaatcaccg
     8341 ataccgcgca tctgcatttc caggcgtggc agcagattgc cgctgaaatt ggcatcagca
     8401 ttgatgcgca gtttaacgaa tccctaaaag ggatcagccg cgatgagtct ctgcggcgca
     8461 ttctgcaaca cgggggcaaa gagggcgact ttaactcgca ggagagggcg caactggcgt
     8521 atcgcaaaaa tctgctctat gtccactcac tacgcgagtt gacggtcaac gctgttctac
     8581 ccggcattcg ctctttgctg gcagatctcc gtgcacagca gatctcggtt gggctggctt
     8641 ctgtctccct gaatgcgccg acgattttag cggcgctgga gctgcgcgag tttttcacct
     8701 tctgcgcgga tgcttcccaa cttaaaaact cgaaaccgga cccggaaatc tttctcgccg
     8761 cctgtgcagg gctgggcgtg ccgccgcagg catgtatcgg cattgaagat gcgcaggcgg
     8821 gcattgacgc cattaacgcc agcggtatgc gctcggtggg gatcggcgcg ggcttaaccg
     8881 gggcgcaatt actgttgcct tcaacggaat cactcacctg gccgcggtta tcggccttct
     8941 ggcaaaacgt atagcaaagg aatcaacatg gctcagcttt cgttacaaca tattcaaaaa
     9001 atctacgata accaggtgca tgtggtgaag gacttcaacc tggaaattgc cgataaagag
     9061 ttcatcgtgt ttgtcggccc gtcgggctgc ggtaagtcga ccaccctgcg catgattgcc
     9121 gggcttgagg agatcagcgg cggcgatctg ttgatcgacg gcaaacgaat gaatgacgtt
     9181 ccagccaaag cacgcaatat agcgatggtg ttccagaact acgcgttgta tccgcatatg
     9241 acggtttacg acaacatggc gtttggtctg aagatgcaaa aaatcgccaa agaggtgatt
     9301 gatgagcggg tgaactgggc ggcgcaaatt ctcggcctgc gtgagtacct gaaacgtaag
     9361 ccgggggcgc tttccggcgg gcaacgtcag cgagtggcgc ttgggcgggc gattgtacgc
     9421 gaagcgggcg tgtttttaat ggatgaaccg ctctctaacc ttgatgccaa gctgcgcgtg
     9481 caaatgcgcg cagagatcag caagctgcat cagaaactga acaccaccat gatctacgtg
     9541 acccacgatc agaccgaagc gatgaccatg gcgacgcgga ttgtgattat gaaagacggg
     9601 attgttcagc aagtaggtgc gccgaaaacc gtttataacc aacccgcgaa tatgtttgtt
     9661 tccggattta ttggatcacc agcgatgaat tttattcgcg gcacgatcga tggcgataaa
     9721 ttcgttacgg aaacgcttaa attaaccatt cccgaagaga aattagcggt tctgaaaaca
     9781 caggaaagtt tgcataagcc catcgtgatg ggaatacgac cggaagatat tcatccggac
     9841 gcgcaagagg aaaataacat ttccgccaaa attagcgtgg cagaattaac cggtgcggaa
     9901 tttatgctct acaccacggt tggggggcac gagttagtgg tccgtgctgg tgcgttaaat
     9961 gattatcatg caggagaaaa tatcactatt cattttgata tgacgaaatg tcatttcttt
    10021 gatgcagaaa cggaaatagc aattcgctaa atacaggggg aaggcattcc cccaggataa
    10081 tacaaggaac aataatgaaa aagttattac cctgtaccgc actggtgatg tgtgcgggaa
    10141 tggcctgcgc acaggccgag gaaaggaacg actggcactt taatatcggc gcgatgtacg
    10201 aaatagaaaa cgtcgagggt tatggcgaag atatggatgg gctggcggag ccttcagtct
    10261 attttaatgc cgccaacggg ccgtggagaa ttgctctggc ctattatcag gaagggccgg
    10321 tagattatag cgcgggtaaa cgtggaacgt ggtttgatcg cccggagctg gaggtgcatt
    10381 atcagttcct cgaaaacgat gatttcagtt tcggcctgac cggcggtttc cgtaattatg
    10441 gttatcacta cgttgatgaa ccgggtaaag acacggcgaa tatgcagcgc tggaaaatcg
    10501 cgccagactg ggatgtgaaa ctgactgacg atttacgttt caacggttgg ttgtcgatgt
    10561 ataaatttgc caacgatctg aacactaccg gttacgctga tacccgtgtc gaaacggaaa
    10621 caggtctgca atataccttc aacgaaacgg ttgccttgcg agtgaactat tatctcgagc
    10681 gcggcttcaa tatggacgac agccgcaata acggtgagtt ttccacgcaa gaaattcgcg
    10741 cctatttgcc gctgacgctc ggcaaccact cggtgacgcc gtatacgcgc attgggctgg
    10801 atcgctggag taactgggac tggcaggatg atattgaacg tgaaggccat gattttaacc
    10861 gtgtaggttt attttacggt tatgatttcc agaacggact ttccgtttcg ctggaatacg
    10921 cgtttgagtg gcaggatcac gacgaaggcg acagtgataa attccattat gcaggtgtcg
    10981 gcgtaaatta ctcgttctga taatgggcta aattgccgga tgcggcgcga gtactttatc
    11041 cgatctataa atgtaggccg gataagatgc gctagcatcg catccggcat tcaggcaagg
    11101 tagctggtat ttatttcagc gtcatatgcg tggcaacggt aatattctgt ggtgacggtt
    11161 ttccagaaat taagcggaat aataactcgc agctttgttg acctaactcc tgcgtcggaa
    11221 catcgatgcc gcccggtgca ggcgttaaaa taaatgacag cgtttcattg ctataaccca
    11281 ccaccgctaa ctgctgcgga atagcaatat ttttctctgc tgccgcacga taaatgctca
    11341 ttaatttcag gctgtcagtg gcaaacaccg cctcaggcaa cggtgactgg cttaataatt
    11401 gccgtgctgc ttttaatgca gtttcatggg tataaccgcc atcaacaatc cattcatcac
    11461 gcactgcaat attatgcgca gccaggctct gcttataacc attaacgcga tcaaccgaaa
    11521 catggacatc aagcggagca tgcaggcagg caatattttg atgcccactt tcaattaatg
    11581 catcggtcaa cgcaatgctg tcgccaaaat tatcggtatc gacagaataa acatgggcat
    11641 attgaccttc aactttgcca atcaccacca ccggaatatc atatttatcg agttgggcaa
    11701 aaaatgactc atccgctggc gaactgagca taataatgcc tttaatcatt ttctgcttaa
    11761 ttttgctttc gcatttttgc agatcttctg ccggattgtg cgacgtttgt aatatcacgt
    11821 cgaaaccttc ttcttcagct ttggcggtga tggcatgcaa aacttcagag aaaaacggat
    11881 tacccgccgt agttttggtc gaacgggtag aaatcaccat aatggcatca aaacccgaag
    11941 aggtcagcgc gcgggccagc ttgtttggct gatactgtaa ttcttcaatg gcccgtaaca
    12001 ctttttcgcg cgcttccggg gagatattag tttgcttatt cagcacgcgt gatacggtgg
    12061 attttgatac gcctgcaacc ctggcaatat cataaatagt aggggacata ggtcaggaac
    12121 tccgtccgaa cgtgaatgct gcacatctta tggagttcgc cggtcgatga caacacgggc
    12181 agttgataat caatggcctg gcccccacat tcatatcctt acgaatgatt ttttttctgg
    12241 tcttcagagc agcacaggac agcaatgaag cgacttaaaa atgaactcaa tgcgctggtg
    12301 aatcggggtg tcgacagaca tctgcgcctc gctgtaaccg gacttagccg cagcggcaaa
    12361 acagcgttta tcaccgcgat ggttaatcag ttgcttaata ttcacgccgg agcacgtttg
    12421 ccgctgttaa gtgcggtgcg tgaagagcgc ctgctgggcg tgaaacgcat tccccagcgt
    12481 gactttggca ttccgcgctt cacctatgac gaagggctgg cgcagttata tggcgatcca
    12541 cccgcctggc caacgccaac gcgcggcgtc agtgaaatcc gcctggcgct acgttttaaa
    12601 tcgaatgatt cgctgctacg ccactttaag gatacctcca cgctgtatct ggaaattgtg
    12661 gattatcccg gcgaatggtt gctcgacctg ccgatgctgg cgcaggacta tttaagctgg
    12721 tcgcgccaga tgacgggctt actcaatggt cagcgcggag aatggtcggc gaaatggcga
    12781 atgatgagcg aagggctgga cccgctagcg cctgccgacg aaaaccggct ggcggacatt
    12841 gccgccgcgt ggaccgatta tctccaccac tgtaaacagc aggggctgca ctttattcag
    12901 cctgggcgct ttgtcttgcc gggagatatg gcaggtgcgc ccgcgctgca attcttcccg
    12961 tggccggatg tcgatacctg gggcgagtcc aaactggcgc aggccgataa gcataccaat
    13021 gccggaatgc tgcgcgagcg ctttaattat tactgcgaga aggtggtgaa ggggttctat
    13081 aagaatcatt ttctgcgctt tgaccgccag attgtgctgg tggattgcct gcaacctctc
    13141 aacagtgggc cacaggcatt taatgatatg cgtctggcac tgacgcagct gatgcaaagt
    13201 tttcactacg ggcagcgtac cctgttccgg cgtttgtttt cgccggttat cgataagcta
    13261 ttgtttgctg ccactaaagc ggaccatgtg accatcgatc agcacgctaa tatggtttca
    13321 ttactgcaac aactgattca ggatgcctgg caaaatgcgg cgttcgaagg gatcagcatg
    13381 gactgcctgg ggctggcgtc agttcaggcg accaccagcg gcattattga tgttaacggt
    13441 gagaaaatcc cggcgctgcg tggtaatcga cttagcgatg gcgcaccgct cactgtttat
    13501 cctggcgaag ttcccgcacg tttgcctggt caggcgttct gggataagca aggcttccag
    13561 tttgaggcat ttcgtccgca ggtgatggat gtcgacaaac cactaccgca tattcgtctt
    13621 gatgctgcgc tggaattttt aataggagat aaattgcgat gaccgaaccg ttaaaaccac
    13681 gtattgattt tgacggtcct ctggaggtcg atcagaatcc taaattcagg gcgcagcaga
    13741 cctttgacga aaatcaggcg caaaattttg ccccggccac gctcgacgaa gcgcaggaag
    13801 aagaggggca agtcgaagcg gtaatggacg cagcgttacg tccgaaacgc agcctgtggc
    13861 gcaaaatggt gatgggcggg ctggctctgt ttggcgcaag cgttgtcggg cagggtgtac
    13921 agtggacaat gaatgcctgg caaacccagg actgggtggc gctgggtgga tgtgccgctg
    13981 gggcattgat tatcggcgct ggcgtaggtt ctgtggtaac agagtggcgg cgcttatggc
    14041 gcttgcgaca gcgcgcccat gaacgcgacg aagcgcgtga tttattgcat agccacggca
    14101 cgggcaaagg ccgcgcattt tgcgaaaaac tggcgcagca ggcgggtatt gatcagtcgc
    14161 atccggcgct gcaacgctgg tatgcctcaa tccatgaaac gcaaaacgac cgtgaagtgg
    14221 tcagcctgta tgcgcatttg gtccagccag ttttagatgc ccaggcgcgg cgcgaaatca
    14281 gccgttcggc ggcggaatca acgttgatga ttgcggtcag cccgctggcg ttggtcgata
    14341 tggcgtttat cgcctggcgc aatctgcgtt taattaatcg catcgccacg ctgtatggca
    14401 ttgaactggg gtattacagc cgtttgcgtc tgtttaagct ggtattgctg aatatcgctt
    14461 ttgccggagc cagcgaactg gtgcgcgaag tggggatgga ctggatgtcg caagatctcg
    14521 ctgctcgttt gtctacccgc gcagctcagg ggattggtgc aggacttctg acggcacgac
    14581 tcgggattaa agctatggag ctttgccgcc cgctgccgtg gattgacgat gacaaacctc
    14641 gcctcgggga tttccgtcgt cagcttatcg gtcaggtgaa agaaacgctg caaaaaggca
    14701 aaacgcccag cgaaaaataa tgcaatatcg ggtgctgacc ggatatcttt acgccgaagt
    14761 gcccgttttt ccgtctttgt gtcaatgatt gttgacagaa accttcctgc tatccaaata
    14821 gtgtcatatc atcatattaa ttgttctttt ttcaggtgaa ggttcccatg cgtctggaag
    14881 tcttttgtga agaccgactc ggtctgaccc gcgaattact cgatctactc gtgctaagag
    14941 gcattgattt acgcggtatt gagattgatc ccattgggcg aatctacctc aattttgctg
    15001 aactggagtt tgagagtttc agcagtctga tggccgaaat acgccgtatt gcgggtgtta
    15061 ccgatgtgcg tactgtcccg tggatgcctt ccgaacgtga gcatctggcg ttgagcgcgt
    15121 tactggaggc gttgcctgaa cctgtgctct ctgtcgatat gaaaagcaaa gtagatatgg
    15181 cgaacccggc gagctgtcag ctttttgggc aaaaattgga tcgcctgcgc aaccataccg
    15241 ccgcacaatt gattaacggc tttaattttt tacgttggct ggaaagcgaa ccgcaagatt
    15301 cgcataacga gcatgtcgtt attaatgggc agaatttcct gatggagatt acgcctgttt
    15361 atcttcagga tgaaaatgat caacacgtcc tgaccggtgc ggtggtgatg ttgcgatcaa
    15421 cgattcgtat gggccgccag ttgcaaaatg tcgccgccca ggacgtcagc gccttcagtc
    15481 aaattgtcgc cgtcagcccg aaaatgaagc atgttgtcga acaggcgcag aaactggcga
    15541 tgctaagcgc gccgctgctg attacgggtg acacaggtac aggtaaagat ctctttgcct
    15601 acgcctgcca tcaggcaagc cccagagcgg gcaaacctta cctggcgctg aactgtgcgt
    15661 ctataccgga agatgcggtc gagagtgaac tgtttggtca tgctccggaa gggaagaaag
    15721 gattctttga gcaggcgaac ggtggttcgg tgctgttgga tgaaataggg gaaatgtcac
    15781 cacggatgca ggcgaaatta ctgcgtttcc ttaatgatgg cactttccgt cgggttggcg
    15841 aagaccatga ggtgcatgtc gatgtgcggg tgatttgcgc tacgcagaag aatctggtcg
    15901 aactggtgca aaaaggcatg ttccgtgaag atctctatta tcgtctgaac gtgttgacgc
    15961 tcaatctgcc gccgctacgt gactgtccgc aggacatcat gccgttaact gagctgttcg
    16021 tcgcccgctt tgccgacgag cagggcgtgc cgcgtccgaa actggccgct gacctgaata
    16081 ctgtacttac gcgttatgcg tggccgggaa atgtgcggca gttaaagaac gctatctatc
    16141 gcgcactgac acaactggac ggttatgagc tgcgtccaca ggatattttg ttgccggatt
    16201 atgacgccgc aacggtagcc gtgggcgaag atgcgatgga aggttcgctg gacgaaatca
    16261 ccagccgttt tgaacgctcg gtattaaccc agctttatcg caattatccc agcacgcgca
    16321 aactggcaaa acgtctcggc gtttcacata ccgcgattgc caataagttg cgggaatatg
    16381 gtctgagtca gaagaagaac gaagagtaag cgcgaatatg cctgatggtg caacaccatc
    16441 aggcatatta aattatgctt tcagtacagc cagagctgct tcgtaatccg gctcggtggt
    16501 gatttcatcc accagctggc tgaaaatcac attgtcattt tcgtcaataa ccacaacggc
    16561 acgcgctgcc agacctttca gtgggccatc agcaattgcc acaccgtagg cttgcagaaa
    16621 ttcagcgtta cggaaagtgg agagggtgat aacgttgttc agaccttctg cgccgcagaa
    16681 acgagactgg gcgaacggca gatcggcaga gatacacagc acaacggtgt tgtcgatctc
    16741 ggttgccagt tggttaaact tacgtactga tgcggcgcaa acaccggtat caatactcgg
    16801 gaaaatgttc agcactttgc gtttacccgc aaactgaccg agggtgacgt cagacagatc
    16861 ttttgccacg agagtaaaag tctgcgcttt gctacccgcc tgcgggatgg aattggcgac
    16921 tgtaaccggg ttgccctgga aatgaacggt ttgtgacatg attatctttc ctgtttacat
    16981 atagttaacg tcacacctag tttatgctaa ctgtcaataa cacagcaaac gctatttgcg
    17041 cttaatccgc agaccaccgc gacaacaagg agtaaagatg agaaccgtta aggtattcga
    17101 ggaagcctgg cccttacata ccccgtttgt gattgcccgg ggaagtcgca gtgaagcgcg
    17161 cgtggtggtg gttgaactgg aagaagaggg tattaaaggc accggcgaat gcacgccgta
    17221 tccgcgttat ggggaaagtg atgcctcggt aatggcgcaa attatgagcg tcgtgccgca
    17281 actagagaaa gggctgacac gggaggagtt gcaaaaaatt ctccctgccg gcgcagcacg
    17341 taatgcgctg gattgtgcat tgtgggatct ggccgcgcga agacagcagc aatcgctggc
    17401 tgatttgatc ggcataacgc ttcccgagac agttatcact gcacagacgg ttgtcatcgg
    17461 tacgcctgat cagatggcca atagtgcatc aacactctgg caggcaggcg cgaaattact
    17521 gaaagtgaag ctggataacc atcttatcag tgagcggatg gtggcaattc gcacagctgt
    17581 gcccgatgcg acgctgatcg ttgatgcaaa tgaatcctgg cgtgcagaag ggttggcggc
    17641 gcgttgccag ctattggcgg atttaggcgt tgcgatgctt gaacaaccgc ttcctgcgca
    17701 ggacgatgcg gcgctggaga attttattca tccgttgccg atttgtgctg atgaaagttg
    17761 tcatactcgt agcaatttga aggcgctgaa agggcgctat gagatggtta atattaagct
    17821 cgataaaacc gggggtctga cggaagcgct ggcgctggcg actgaagcgc gtgcacaagg
    17881 tttcagtctg atgctgggct gcatgttgtg tacctctcgt gccattagcg ccgctttacc
    17941 gctggtgccg caggtcagtt tcgccgatct tgacggaccg acctggctgg cggtagatgt
    18001 ggaaccggcg cttcagttca cgacgggcga attgcatctt taggatgcca gcgcagcaag
    18061 tttgccatcg caaagagata tttttcgctg gcttcgtcgg aggagatggg cggaaactct
    18121 gcggtgatgc aatgcaagtt cagatctgca caccaactgc cgaaagagcc aggcgtttca
    18181 taaccgacgc tggttaccag aggcaattca aacgcctgag ccagccattc gcctaattcg
    18241 ctgtgtctgg gatcttcaat acaggccagt ggatcgtgga atgagaccac ccaggcaggt
    18301 tgaatgcggt gtataagctg gcacagcgcc tgggtttcag gttcggagcc aggtttgtca
    18361 cctgtcagta atacgacatc gcgttcttca gcggcgctgt tccagcgata aacggtttca
    18421 ccttccttcc agttcgccgc cggaaagttt ctgtttaaat ccacaccatt agcattggcc
    18481 cgtaacccca actggcagcc gtcaggattc acacacagca ccacatgatg acggcgcaaa
    18541 gaaggtgtca acgtccgcag agcacaggag agcgtgacga ccgaagagtt ttcatcaccg
    18601 tgagtgccag ccagaatcaa accactttcg cggctggctg caggggcagg aaaccagatt
    18661 aacggcgcgc ccaataatga acgtccgtaa tgttctgttc cgggcggaaa tgcgccgcgt
    18721 tcggcgcgtg ggcgggttac ggtcatgatc gtctctggtt caggaagtat tacaggcagt
    18781 gttgtgcaaa ttttgcggat tatcaaatcc ttttcttcaa cgctatgtca tcgatcttgt
    18841 atcacttttt tgccggaagt tgtttgcatt ttattagttg gatagggttt gtggattttt
    18901 gctggctgac gggcaaataa agtttgttta atggtttgct tatctgccag ttgattaatc
    18961 acatggcggg ctatctgacc gccagcacca agaatcagta catttttcat gaaaattatc
    19021 ccagattatt ggcagcaata tgccaggcca tatcactatt gcggatttga atatgtttca
    19081 ttacagagtt tcctcaaaga acggagctaa tactgaaatt gcttcagcca cataggcttt
    19141 gccgtcataa agatccatat ggtttgcgcc ttcaacaatg tgatagcgtt tatcctggct
    19201 tgatgctcga tcgtacaggt cgtcactcat ccatttgctc cctgcctggc tgcccgccac
    19261 aatctgcgtc ggctgagtca ggtacacttc cgccatatgg taagcatcat aggtaataat
    19321 ctggttaagg ctgcgcaaag tagcgtaacc cggtgctgtt ggatactgcg cgcgaggggt
    19381 gtggtaatac tcccaggcct gacgcagttc ttcgttcggc gcatcggact ccttcattgg
    19441 tgccagtggc ataatggcgt attctccgct gctaatatcg ctggttctgg cgtttgaacc
    19501 cgcttcaacg tatggcaggg catcaataga tttcacattg ttttcccaac cattacggaa
    19561 tatcgaacca atattgaccg cactaacggt accgatggcc ttgatgcggc gatcctgaat
    19621 tgcagcattg gctgtatatc ctgcaccggc acaaattccc atcgcaccaa ttcgggtatt
    19681 gtcgacataa gaaagcgttg tcaggtaatc aatcacggca ctgatgtctt cagtacgaat
    19741 gtatgggttt tctaactgac gcggctcgcc gccactttca ccttgataag atgcgtcata
    19801 agcaatagtg acaaatccct tttccgccag ttttttggca taggttccgg ccgtttgttc
    19861 tttaacgccc ccacctgggt gagataacac aattgcctga tactgacggg tttcatcaaa
    19921 ttttggaggg aaatagatca ctgcagacaa agagatggtt ggattattgc tgttagtgaa
    19981 gctgacttta ttattcatca ttccgttcct catggagttg tcggttcgtt ttaacggttg
    20041 gtgatatcac tatagatatt gatcattaag ttgattagac ccaaaatcat gattagacct
    20101 atcatttaaa tgattaatag attgcatgga gatactgaat gaagcgtgaa gaaatcgctg
    20161 atctgatggc gtttgtcgtc gttgcagagg agcgtagctt cactcgtgca gcagcccgcc
    20221 tgagcatggc gcagtcagct ttaagccaga tagtgcgtcg tatagaagaa cgattgggat
    20281 tgcggcttct gacgcgaacc acgcgcagcg ttgttccaac tgaagcgggc gagcatcttt
    20341 tgtctgttct tggccctatg ttgcatgaca tagattcagc catggcatcc ctgagcgatc
    20401 tgcagaaccg cccatccggg acaatacgta ttactactgt agaacatgca gcaaaaacga
    20461 tattgttacc agcaatgcgc acattcctga aatcgcatcc tgaaattgat attcagctca
    20521 ccattgatta tggtttgacc gatgtcgttt ctgaacgttt tgatgcaggc gtccgtctgg
    20581 gtggggagat ggataaagat atgatcgcca ttcgaatcgg gccagatata ccaatggcta
    20641 ttgttggctc accggattat ttttctcgcc gaagtgttcc aacgtcagtg tcacaattaa
    20701 tagatcatca ggcaattaat ttgtatcttc ccacatcggg tacagcaaat cgctggagat
    20761 taatacgcgg tggacgtgaa gttcgtgttc gcatggaagg tcagctttta ctgaatacga
    20821 tagacctgat cattgatgct gcaattgatg ggcatggatt ggcgtatcta ccttatgatc
    20881 aggttgagcg ggctattaaa gaaaaaaaac tgatacgtgt tttggataaa ttcacaccag
    20941 atttacccgg ttatcacctg tactatccac accgtcgaca tgctggctcg gcattctcat
    21001 tatttataga taggctgaag tataaaggtg ctgtttagca ctacttgctg atacattaat
    21061 ttaattcttc tcttaacgta ttctcagttc ctttcaacgt tttggtcatt ttttattctt
    21121 cgtacaatgg cgacagatgc tgattatgat accgaaaacg ggtttgaacg tgcgaagccc
    21181 gaacgtagtg ttcggagttc tatgtgcttt accgcatttt ggagactatt atttacacta
    21241 aatctgattt gatatattga tacttaaaac atttgatgct tcctttgtca cttttttgat
    21301 ggaagttgtt tgcatttctt taaggcgaaa caaataatta cgcatcaatt ttaatgtcgg
    21361 ttagagggaa acttatgaag cactctgttt cagtcacgtg ttgtgcgctg ttggtcagca
    21421 gcatttctct ttcgtatgct gcagaagttc cgagcggcac agtactggca gagaagcagg
    21481 agctggtgcg ccacattaaa gatgagcctg cgtcgctgga tcccgctaaa gccgtgggcc
    21541 tgccagagat tcaggtcatt cgcgatctgt ttgaaggtct ggtgaatcag aacgaaaaag
    21601 gggagattgt ccccggcgtt gcgactcagt ggaaaagtaa tgacaaccgt atctggactt
    21661 ttaccctgcg cgataacgca aaatgggcgg atggcacacc ggtaacggcg caagattttg
    21721 tctacagctg gcaacgtctg gtggacccaa aaacattgtc gccatttgca tggtttgccg
    21781 cgctggcggg aatcaacaac gcacaggcga ttattgatgg taaagctacg cctgaccagc
    21841 ttggcgtcac cgcagttgat gcccatactt tgaaaattca gcttgataaa ccgttgccgt
    21901 ggtttgtgaa tttaaccgct aactttgcct tcttcccggt gcaaaaagcc aacgtagaaa
    21961 gcggtaaaga gtggacgaaa cccggaaatc tgatcggcaa tggcgcttat gttcttaaag
    22021 agcgcgtagt caatgaaaaa ctggtcgtgg taccgaatac ccattattgg gataacgcca
    22081 aaacggtact gcaaaaagtg accttcctgc caattaatca ggaatccgca gccactaagc
    22141 gttacctcgc gggggatatt gatatcaccg aatccttccc gaaaaatatg tatcagaagc
    22201 tgttgaagga tattccgggg caggtttata cgccgccgca gctcgggacc tattattatg
    22261 cgtttaacac gcaaaaaggg ccgacggcag atcagcgcgt tcgtctggca ttaagtatga
    22321 cgatagatcg ccgcctgatg accgaaaaag tattagggac gggcgaaaag ccagcgtggc
    22381 attttacacc agatgttacc gcgggattta cgccggaacc ttcgccgttt gaacaaatga
    22441 gtcaggaaga actgaatgcg caggcaaaaa ctttgttgag cgcagctggt tatggtccgc
    22501 aaaaaccgct gaagctgacg cttttgtata acacttcaga aaaccatcaa aaaattgcga
    22561 ttgctgtagc atcgatgtgg aaaaagaacc ttggcgtaga tgttaaattg caaaatcagg
    22621 aatggaaaac ctatatcgat agccgtaaca ccggcaattt tgatgttatc cgcgcctcgt
    22681 gggtggggga ttataatgaa ccctccactt tcctgacatt attaacgtca acgcattcag
    22741 gaaatatttc acgctttaac aatccggcat atgacaaagt tctggcccag gcatcgacgg
    22801 aaaataccgt taaagcgcgt aatgccgatt acaacgcggc agaaaaaatc ctcatggagc
    22861 aagcaccgat tgcaccaatt tatcaatata ccaatggacg attaatcaag ccgtggctga
    22921 aaggttatcc cattaataat cctgaagatg tggcgtacag tcggactatg tatattgtga
    22981 agcattgatg tgatgggaac tggcgttacc cttgtgcata acgccagtga tgtctgattt
    23041 agcgaccctg ttccggcggt gtaatgttat ccatatacag cgtctggctg gggaaggcaa
    23101 agtccgcgcc gtgtgactgt acaatatcga taatcttcaa ataaacgtct tgctgtgcag
    23161 caagccattc agcccatacc gtggttttgg taaagcaata aaccataata ttcaatgaag
    23221 agtcagcaaa ctggttgaaa taaaccagta aggtttgtcg ctggtcgatg gccgggtgat
    23281 ttttcagcat ctcacgtaca gcttcgacaa taacgcccac ttttgccgca tcctcataac
    23341 gtaaaccaat ggtcgtggta atgcggcggt tggtcattcg tcctgggttt tctacgctga
    23401 tcgacgaaaa cagcgagttc ggtacgtaca atggacgatt atcaaaggtc gtaattttgg
    23461 taattcgcca gccaatttcc gctactgtac cttcgatatt tctgtccggt gaacggatcc
    23521 agtcgccaat actgaaagga cggtcgaaat agagcataat cccggaaaag aagttactca
    23581 gaatatcttt accggccata ccgacagcca gaccaccaat accaccaaag gtcagcaagc
    23641 cagaaaggct catgccgaaa tgttcgccat aaagcagaac aagcaccaca ataatggtga
    23701 ttttgatgat acgcgacata atccgcgcac tggtgatatc gcgacctttt ttaatctgct
    23761 gtttttcaaa ctgattaatc agcagaaata gcttaatcgt cagaataacc gcaatcaggg
    23821 acgtacagat aaaatcgata acgcctgggg tgataaattt gagtttatag ttttctataa
    23881 cataattaat aatgctacca acagcactga taattatggt gtagattaaa aattgcaccg
    23941 catggaataa aaatcctttt cttttacgat ttccacggcg aaaccaaaag ctcatcagaa
    24001 tcaatgctgc gcagctaccg aaaataatga ccagattaag cgcattattt gtaaacagtt
    24061 cagcgatcat tgttttatca ggctcctcca gataattgtc gtcatgccgg aaacccctgg
    24121 cggggctatt ttaccgcgac aattcattca gatcatcaat agtcagggaa ggaagtagca
    24181 acattagcta actaatcaat gcgttgattg taaatccagc taagaggtga ggttttcaga
    24241 gcagacaacg gtgaaatgtc atggtattgt tacgtttagg taacaagaaa tttgtctgca
    24301 caaggattac atcatgatta tggcgaaact gaagtcagcg aaagggaaga aatttctctt
    24361 tggtttgttg gcggttttca ttattgcggc gtcggttgtg actcgcgcga ccatcggcgg
    24421 cgttatagaa cagtacaata ttccgctgtc tgagtggacg acatcaatgt atgtgattca
    24481 gtcatcgatg atttttgttt atagcctggt ctttactgtg ttgctggcaa tcccgttggg
    24541 aatttatttc cttggcggcg aagagcagta agtaaaaaat aggcccgata actcgggcct
    24601 tgtcagttat tgaagagtcg ttaatcgtct tcttcgtcat ccagttcaac gggtgtctga
    24661 tactggtcag gtttaatgac cagcaggtcg cagcgaagat gatcaatcac ctgttccgcc
    24721 gtgttgccga ggaatgctgc tgaaataccg gtgcgtccga ccgtgcccag aaccacaatt
    24781 cccgcctgta aatgctccgc caaatcagga atcacctctt caggcagacc tttttctacg
    24841 tgcgtcatgt tttcattaat gccgaatttc tgccgcagag ctttcattgc cagcaaatgt
    24901 tgcccacgaa tggcatcgtt ataaacgctc gggtcaaatt ccggcagttc aatcgcgata
    24961 ttaattggcg ttaccggata agcgccaacc agatgaactt cggtatggtt gacttgttct
    25021 gccagttcga tcgtctcttt gaccagtttt tcattgagcg cattatgata cggctcttca
    25081 ctggcgagat tcaccgccac cagcgccttg cctccttccg gccacggctg gtctttcacc
    25141 atccacaccg ggcttgggca tttgcgtaac agatgccagt ccgttggcgt aaaaatcacc
    25201 gcttccagac ggtcatgttg gtgcgccatt tttagcacca aatcgtgtcc gccgctgatc
    25261 acttcctgaa tgatggcttc gaaaggacgg ttatgccaga ccactttaat ttcaatggga
    25321 acgccagcat tgagataata ttttgcctgc tcgtggatcc aggctgtacg ctggctgatg
    25381 acgccctgac gcatagcggt acgttcgtcc ggggagagca gggtggtcat ttcgtatgag
    25441 aagtcataga tcggcaaaaa ggctttaatt ttgccaccaa tccgttgatg taaataaaca
    25501 gctcgccgca atgctggttg gtcgtcctgg ttaggatcga taacaacgag catgttctga
    25561 tacatagcca tacagggtct ccttacaaca actgtcaacg cagtttgtaa ttaaaagatt
    25621 aacccatatc tggtgaatga aacagtgatg aaccttctgc cagatcaata aatcagaaaa
    25681 atttaatgat atgacagaag gatagtgagt tatgcggaaa aatcaggcaa cgttacgcgt
    25741 atgaccagca agctgggcca gcgcatcgtt attttcgatg gtgatgtatt tacctttgac
    25801 tgccagcata ccgcttttct ggaagcgacc cagcagacgg ctgatggttt ctaccgtcag
    25861 gcccagatag ttaccgatat cgccacgagt catcgtcagg cggaattcac gaggggagaa
    25921 gccgcgttgg gcaaaacgac gggacaggtt gtagatgaat gcagccagac gttcctcggc
    25981 atttttcttc gacaacagca ggatcatgtc ctgatcgcct ttgatttcac cgctcatcag
    26041 acgcatcatc tgctgacgca gattcggcat tttaccggac aaatcgtcca gcgtttcgaa
    26101 cgggatttca cataccatcg aggtttccag cgcctgagcg aagctcgggt gatggccgct
    26161 gccgatagca tcaaatccca ccaggtcacc tgctaaatga aaaccagtga tttgctcgtc
    26221 gccttgctca gtgatggtat aacttttaat cgtaccggag cggatggcat aaagcgattt
    26281 aagttcatca ccagccttaa acagcgtctg gcctttctga ataggcttct tccgctcaat
    26341 gatattatca agctgatcaa gctcatgttc gttgagtgtg aacgggatgc aaagctggct
    26401 gatgctgcaa tcctggcaat ggatagcaca accgccagac tgaatgcgcc gtataattcg
    26461 cttttccggg atcataggtc tgctcaagcc gtaattgata tttgtcaatt ttaacatctt
    26521 tttagggagc aagtaagtct aagcaaacct taacagcaga gaattccgat attagatgta
    26581 aatatatgtc tatctatttg aaaaccatta agttgttaag ggtaacttta cataaaagtg
    26641 tgaacaagct ggcacaaatt gtttaatgtt tacagcaaaa gataaccttc atggcgcaat
    26701 aaccactctt ttcgctgaac tccgcctgca tatccggtca tggtgccgtt tcggccaata
    26761 acccgatggc aaggtacgac gatgctgatg ggattcgatc cgtttgccgc accaacggca
    26821 cgcgccgcgc cagggcggcc caattgttca gccagttggc cgtaatgcat tacctgcccg
    26881 caggggatag tgcgtagtgt tttccagact tcgcgctgaa atggcgtccc ccctgtggca
    26941 gtgggaagcg tatcaataat gctaagatta ccggcaaaat aatcacgaag cttgtcgctt
    27001 aaaccgcctg ggttggtggc agaaatgcgc tcatagcctt ctttgcgata atggatgtcc
    27061 agcagctgca ccatgcgttc gctgtactct tcccattcaa ccgcccgcag acgaaattgt
    27121 tcatcgcaaa tcacccacag tggacccagt ggcgtggcaa ttttttcttc aagtaatctc
    27181 agcatccgtt ctctcttaag acaaacgtgg ggaaatcccc ggacctatcg gcagaccaac
    27241 aagataccac gccagcaaca tcagcagcca taccaccaaa aagataagcg gatagggcaa
    27301 gactaacgaa tagtaagtac ccagtttcgc gtctggtttg tagcgttgca ggaatccaag
    27361 aaacagtgga acaaaaggag ataccggcgc taaaggcaat acggatgagt cggcaatacg
    27421 aaagaggatt tgcgcaaatg ccgggtgaaa gccaagtagc ataaacattg gtacgaaaat
    27481 gggggcaaga atcgaccaga ttgcggaacc gctggcaata aacatgcata agaaagagga
    27541 aagcaacgcc agaccgacaa acgccgggat gccgctaagc cctgaacttt ccagtatatc
    27601 ggtcagcccc acggcgatga atttccccat gttgctccag ttaaacatgg cgacaaattg
    27661 ggcgagggga aaaaccatca cgataaatcc cgccatctct ttcatcggtt caatcattaa
    27721 atgcggtaaa tccgcctgac gtcgaattgt gcgggtagcg atgccataag ccagcgagac
    27781 aacaaagaaa aaaagaatga tcagtggcac gataccttta ataaagggtg atggcatcac
    27841 ggtgtgatta atcggatcgc gcaatatccc gttttgcggg atcaccatca gcgcaatcgc
    27901 agcaataaaa agtagcgata cgacacctgc tatgcgtaaa ccaaaacgct gactttcggt
    27961 caatgtctgc agtttctcat cgctgtttcc ctgccattga cctaaccgtg gctcgatgat
    28021 tttgtcggtt atcaggccgc caacaatcgt cagtacgact acggagctgg ccataaaata
    28081 ccagttatca attacactga cgtgcatttg cggattgaac gcagctgccg cttccgtgct
    28141 gatccccgac agcaacacgt cggttgtgac aatcagtaaa ttagccgtaa agccgcaacc
    28201 tacgcctgca atggcagcca gtaaacctgc aactggatgc ctgcccaccg ccagaaaaat
    28261 cagcgcaccc atcggtggca tgatcactaa cgccgcatcg gaagaaatgt ggctgaaaaa
    28321 agcaataaac agcaccatat aactggcgta gcgggcatta acatgcgatg ccattttaac
    28381 cattagtgct ggcagtaagc cgacgcgctc cgccagaccg gcacctaaaa ccagcgccag
    28441 gatcgcacca agtggagcaa aaccgctaaa gtttttaata acattgggta aaaaccagtg
    28501 taatccttcc acactgagca ggtttttcac caccaccggc gtaccatcgg tcgggttttc
    28561 gcactgacgc caaaggccga caaaattgcc gtcgtcacca tgagtacgat aatcaaatag
    28621 ataaagagca gaaaaggatg gggaaccttg ttaccaattc tttcgaccca gccatagagc
    28681 ttcccggatt gggaggacga cggtatggat gacatactca tgggcattcc tcggttgttg
    28741 tgtttgcgtt gttgttgtta ttttaaaggt gacggtgtca cgtttttcgg gatagggcag
    28801 tgatacggtt gcgtgtccgt tacttgctga tgctcttgtt ggcattcttg caatagccct
    28861 gaatcaagga agagattgac tgtggttgct gccatagttt tcgccgccag cagcattcct
    28921 ttatgagcaa tagatgttcg cccctggcta accagttgcc acgtatgtag cggtgtaccg
    28981 acggcaaaac aggggctgaa acactgggca acaggcagtt tccaactgac gtcgccgaca
    29041 tcagtcgatg ccgcaagcac gttatcggtg gcggcatatg gagcgacttc attcgccagt
    29101 accgtttcac gatgacgtag tgcaaaaacc ttgccgtttt cgccaccggt tgcggcgata
    29161 ttattcagac tgttttgccg atcgttggag gtgagcgtag cctgaatttg tttcgcaaaa
    29221 gccagttctt cggagttcca ttccggggta ccaaaatggg atagggcctg gtacatggca
    29281 ttttctaagg tgcgattcgg gagataactg gaacaggctt tgtcgaagcg gcattcaacc
    29341 gtggtttcgg tcatcaatgc cgcaccttcg gcgattttgg cgacccgatc ataaatatgc
    29401 tgcacgtcgg tcatttcggg ggcgcggata agataaagca cttctgcctg cgcctggacc
    29461 acgttgggcg agatcccgcc gctatttgtg atggcatagt gtacgcgcgc tttttcaata
    29521 atatgttcgt tgaggaagtt ggtgccagtg gtcatcaacg ttacggcatc aagggcgcag
    29581 cgtcccaaat gaggggaatt cgcggcatgt gctgcgatcc ctttaaagcg ccatgatgcc
    29641 tgaatgtttg ccagcgtgcg ggtattgaac ataccggcaa aggcttccgg gtgccaggtg
    29701 agtgccgcat ccacatcatc aaataccccc tcgcgaacca tgaacgtttt acccgagccg
    29761 ccttcttcgc caggacaacc ataaaagcgc accgtgccgc cttgcccata ttgttccagc
    29821 catttcttga cggctattgc agcggcaaag gcggcggttc ccagcaaatt gtgtccgcaa
    29881 ccgtgaccat tttcaccggg cgtcacggat gtaggttgcg cgcaacctgc ttgctgactt
    29941 aaacctgcca gggcgtcata ttctcccagc agggcgataa ccggtttgcc ttgaccaaac
    30001 gaagcaataa aggcatttgg gatattgcct acgttgcggg taacggtgaa gcctgcagat
    30061 tccagcgccg aagccagatg ctccgctgac cagaactctt caaaacgtgt ttctggatga
    30121 tcccagattt gatcggcaat atcggtataa cgttggcgat cggcttcaat cgcatcgtcg
    30181 ataaaacgat agatttcctg catcagatac ctcgcgtcca gggaaaattg agcgcggtgc
    30241 gcgccagcgt ttcgacggca atagcgagaa cctgctcgtc aaaatcgaat ttttcgttgt
    30301 gatgacctgc cgccagctgt gtgccaaaca ccacgtagga ggcttgccct tgatgttgct
    30361 gcacgcgggc catcattaat gtggcatctt cggaacccgc aggcgcttca acacgttcaa
    30421 tggcctgatt gacccccgcg acctgagccg cctgactttg caaccatgcg acccattgcg
    30481 gcgaaggaga actggcggta gctgcaccca tcagacgagt ttcaacgccg acaccataca
    30541 tggttgctgc gccctgaatc gcttgttgtg cacggtcaaa aacatattga ttaatgacgt
    30601 cgctggcccc gcgtgtttcc actttcagca acgccgaggc aggaacaacg ttacgaccgc
    30661 ttcctgcctg cataacgccc acgtttactc tggaagctcc ttcgctgtgc ggggcgattg
    30721 catgcagtgc aagagtggct tgtgctgccg ccaacaaggc attgtgaccg tcttctggtt
    30781 ttgcgcctgc gtgagcggcg gtaccggtga agtgcgcgtc aaatttggtg gttgccataa
    30841 aattatcact gccgcacacc acggtgcccg caggtacgcc agtgccaatg tgcacggcag
    30901 taaaataatc aacatcatct acgacacctg catcgaccat cgcccgcgcg ccacgcgtac
    30961 cttcctctgc aggctgaaaa atcagtttga tgacgccatg tagtccggac tcgaactgtt
    31021 taagggtatg cgccagccca agcccaatgg cggtatgtcc atcatgacca caggcatgca
    31081 tcattccggc gttacatgac gcaaaaccgt cgcggtaggg gcgatggctg acatcctgct
    31141 cttcactgag atccagcgcg tccatatcga cacggaaagc catcaccgga ccggggcgac
    31201 cggtatccag ggtggcgacg ataccagtga aaccaccttc aaaagccgca atccattgtg
    31261 ctagcgcacc ctgttgacga gcgcgctcga attcgcgttg tagagtgaat tcatcaggta
    31321 atcccatccg gctactttca ttaactactt cgcgacccag cgccagtgaa tagccgagct
    31381 ggtgcaattc ttccgcaaca agggtggcag tgcggaattc cacccagcca gactctgcat
    31441 agtgatgaaa atcacgtcgc cagtgcgata attttggggc aagcgaatta acaaattgat
    31501 tcaaagactc cataaccttt cccgtcatca gtaaaaagtg tgacccggtt cacgtagcga
    31561 tagtttttac ttatcactaa ctgatttttc acagttttaa ccgttcataa attaccctga
    31621 cacaatcatc tgcattaaag tagatgccag tttctttggt ctgataaata acggttatcg
    31681 gtggcgtcat ggcttttcag gtaaaaattc atcaaattcg ggcttttgtt gaagtggctc
    31741 gtcagggcag cattcgcgga gcgagccgaa tgttgaatat gtcgcaaccg gcactgagta
    31801 aatctattca ggagctagaa gaagggttag cggcgcaact cttttttcgc cgtagtaaag
    31861 gcgtgacgtt aactgatgcc ggtgaaagtt tttatcagca cgccagtcta attcttgaag
    31921 agctgcgcgc agcccaagag gatattcgcc aacgacaagg gcaactggca gggcagatta
    31981 atatcggcat gggggccagt atttcccgca gtctgatgcc agctgtcata tctcgttttc
    32041 atcagcagca tccgcaggta aaagtacgca ttatggaagg gcaactggtg tcgatgatta
    32101 atgaattgcg tcagggagaa ttggatttca ccatcaatac ctattatcag ggaccgtacg
    32161 accacgaatt tacttttgag aaattactgg aaaagcaatt cgcgatcttt tgccgcccgg
    32221 gacaccccgc cattggtgcc cgttcgatca aacagttact ggattacagc tggacaatgc
    32281 cgacgccaca cggcagctac tacaaacagt tgagtgaatt gcttgacgat caggcgcaaa
    32341 cgccacaggt cggtgtagtc tgcgagacgt tctcagcctg tatcagtctg gtggcaaaaa
    32401 gcgattttct cagcatactg cctgaagaaa tgggctgcga tcccttgcac ggacaggggc
    32461 tggtgatgtt gccggttagc gaaattttac cgaaagcggc ctattatttg attcagcggc
    32521 gtgatagtcg ccagacacca ctgaccgcgt cattaatcac gcaattccgg cgagaatgcg
    32581 gctatctgca aagttaaaac tgcataaaaa aatagagtct gtcgacatcc gccagactct
    32641 acagtacaca cagcagtgca tccgcgtctt aaatccggca ttgtctcctc tgcgccggtg
    32701 actgtgttat aacggtttta acctttagtt gccaattttc ttcagtgaca gat
//
LOCUS       DAPHBJ010000030        32022 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.030, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000030 DAPHBJ010000000
VERSION     DAPHBJ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.030"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(52..942)
                     /gene="rarD"
                     /locus_tag="RHZ76_001305"
     CDS             complement(52..942)
                     /gene="rarD"
                     /locus_tag="RHZ76_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDV2811969.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(994..1461)
                     /locus_tag="RHZ76_001306"
     CDS             complement(994..1461)
                     /locus_tag="RHZ76_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HDV2811970.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1626..2495
                     /gene="pldA"
                     /locus_tag="RHZ76_001307"
     CDS             1626..2495
                     /gene="pldA"
                     /locus_tag="RHZ76_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HDV2811971.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNCLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2628..4457
                     /gene="recQ"
                     /locus_tag="RHZ76_001308"
     CDS             2628..4457
                     /gene="recQ"
                     /locus_tag="RHZ76_001308"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDV2811972.1"
                     /translation="MAQAEVLNLESGAKQVLQDTFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKPGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4521..5141
                     /gene="rhtC"
                     /locus_tag="RHZ76_001309"
     CDS             4521..5141
                     /gene="rhtC"
                     /locus_tag="RHZ76_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HDV2811973.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQIELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5203..5823)
                     /gene="rhtB"
                     /locus_tag="RHZ76_001310"
     CDS             complement(5203..5823)
                     /gene="rhtB"
                     /locus_tag="RHZ76_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HDV2811974.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5934..6956
                     /gene="pldB"
                     /locus_tag="RHZ76_001311"
     CDS             5934..6956
                     /gene="pldB"
                     /locus_tag="RHZ76_001311"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HDV2811975.1"
                     /translation="MFQQQKDWETRENAFAAFTLGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6964..7764
                     /gene="yigL"
                     /locus_tag="RHZ76_001312"
     CDS             6964..7764
                     /gene="yigL"
                     /locus_tag="RHZ76_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HDV2811976.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFQYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7840..8739
                     /gene="bioP"
                     /locus_tag="RHZ76_001313"
     CDS             7840..8739
                     /gene="bioP"
                     /locus_tag="RHZ76_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HDV2811977.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNTQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8627..9580)
                     /gene="metR"
                     /locus_tag="RHZ76_001314"
     CDS             complement(8627..9580)
                     /gene="metR"
                     /locus_tag="RHZ76_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HDV2811978.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9698..11959
                     /gene="metE"
                     /locus_tag="RHZ76_001315"
     CDS             9698..11959
                     /gene="metE"
                     /locus_tag="RHZ76_001315"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDV2811979.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(11999..12814)
                     /locus_tag="RHZ76_001316"
     CDS             complement(11999..12814)
                     /locus_tag="RHZ76_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDV2811980.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAASAEDGWQRMLEWFKQYGGKKSL"
     gene            13076..13837
                     /gene="udp"
                     /locus_tag="RHZ76_001317"
     CDS             13076..13837
                     /gene="udp"
                     /locus_tag="RHZ76_001317"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HDV2811981.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            13978..15405
                     /gene="rmuC"
                     /locus_tag="RHZ76_001318"
     CDS             13978..15405
                     /gene="rmuC"
                     /locus_tag="RHZ76_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HDV2811982.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15500..16255
                     /gene="ubiE"
                     /locus_tag="RHZ76_001319"
     CDS             15500..16255
                     /gene="ubiE"
                     /locus_tag="RHZ76_001319"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HDV2811983.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16269..16874
                     /gene="ubiJ"
                     /locus_tag="RHZ76_001320"
     CDS             16269..16874
                     /gene="ubiJ"
                     /locus_tag="RHZ76_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HDV2811984.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            16871..18511
                     /gene="ubiB"
                     /locus_tag="RHZ76_001321"
     CDS             16871..18511
                     /gene="ubiB"
                     /locus_tag="RHZ76_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HDV2811985.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18590..18859
                     /gene="tatA"
                     /locus_tag="RHZ76_001322"
     CDS             18590..18859
                     /gene="tatA"
                     /locus_tag="RHZ76_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HDV2811986.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            18863..19378
                     /gene="tatB"
                     /locus_tag="RHZ76_001323"
     CDS             18863..19378
                     /gene="tatB"
                     /locus_tag="RHZ76_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HDV2811987.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19381..20157
                     /gene="tatC"
                     /locus_tag="RHZ76_001324"
     CDS             19381..20157
                     /gene="tatC"
                     /locus_tag="RHZ76_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HDV2811988.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20199..20981
                     /gene="tatD"
                     /locus_tag="RHZ76_001325"
     CDS             20199..20981
                     /gene="tatD"
                     /locus_tag="RHZ76_001325"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HDV2811989.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVACAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(20978..21466)
                     /gene="rfaH"
                     /locus_tag="RHZ76_001326"
     CDS             complement(20978..21466)
                     /gene="rfaH"
                     /locus_tag="RHZ76_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HDV2811990.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21633..23126
                     /gene="ubiD"
                     /locus_tag="RHZ76_001327"
     CDS             21633..23126
                     /gene="ubiD"
                     /locus_tag="RHZ76_001327"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HDV2811991.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWSEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23172..23873
                     /gene="fre"
                     /locus_tag="RHZ76_001328"
     CDS             23172..23873
                     /gene="fre"
                     /locus_tag="RHZ76_001328"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HDV2811992.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24156..25319)
                     /gene="fadA"
                     /locus_tag="RHZ76_001329"
     CDS             complement(24156..25319)
                     /gene="fadA"
                     /locus_tag="RHZ76_001329"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HDV2811993.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPE
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25329..27518)
                     /gene="fadB"
                     /locus_tag="RHZ76_001330"
     CDS             complement(25329..27518)
                     /gene="fadB"
                     /locus_tag="RHZ76_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HDV2811994.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27708..29039
                     /gene="pepQ"
                     /locus_tag="RHZ76_001331"
     CDS             27708..29039
                     /gene="pepQ"
                     /locus_tag="RHZ76_001331"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HDV2811995.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29039..29653
                     /locus_tag="RHZ76_001332"
     CDS             29039..29653
                     /locus_tag="RHZ76_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HDV2811996.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29692..31143
                     /gene="trkH"
                     /locus_tag="RHZ76_001333"
     CDS             29692..31143
                     /gene="trkH"
                     /locus_tag="RHZ76_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HDV2811997.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31155..31700
                     /gene="hemG"
                     /locus_tag="RHZ76_001334"
     CDS             31155..31700
                     /gene="hemG"
                     /locus_tag="RHZ76_001334"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HDV2811998.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7420 a         8183 c         8770 g         7649 t
ORIGIN      
        1 tgcccactct aatgcaagag tcggcatatt ttttcagaat atatttattt tttatttgga
       61 cgttctacgc tgcgtataaa tcgcatccat cacaaaaatt gccagcgcca cccaaataaa
      121 ggcgaaagtc accatcttat cggcacccgg tttttcacca taaaacgtca cagccagcag
      181 gaacatcagc gtcgggccaa tgtactggaa aaagcctaac gttgagagac gcaagcgcgt
      241 ggcagcggcg gtaaaacaca acagcggtac ggtagtgaca ataccggcgg cgatcagcag
      301 taaattcagc gacatcgggt tttgccccat atggctggtt gagctgtcgg caatagcaaa
      361 caggtaaatt gccgccacgg gcagcagcca catggtttca attaacatgc cggtttgcgc
      421 ttcaacggca atcttcttgc gtaccagacc gtagaaggca aaactaaatg ccagtcccag
      481 cgcgataata ggtagagaac caaaagtcca cagctggact aacacgccac atatcgccag
      541 aatcaccgcc agccattgca tccggcggaa tcgctcgccg aggaaaatca tccccagcac
      601 aatgttcacc agcgggttaa taaagtaacc aaggctcgct tccagcatat ggtgattgtt
      661 caccgcccag ataaacagta gccagttgcc accaatcagc acggcagaga ctgccagcat
      721 aaaaattttc tgtggcgtct gaatcagcgt ttttaaatag gaccactggc ggcaaatgct
      781 catcagcacc accataaaga aaaacgacca gatcacgcga tgcgtcagga tttcatcggc
      841 gggcacgtag taaatcaact tgaagtacgc tggcgctata ccccaaataa aataagcggc
      901 aagagcgagt aatacgccct gccgcgtttg ttttgcatcc atcgggaata ctcattttta
      961 attggtaaca gcagtttacc tgcttttatg tcttcaacct accatatagg tggcggtggc
     1021 actggcaata taaagctgtt cttcattgtg taattcaacg cgggcgacgg cgactttatt
     1081 gcctgcacgc aacaggctac tggttgcagt aaaacgctcg cccctgcctg ggcgcagata
     1141 atcaacgcga agatcaatgg tccccatccg tgatagccgc tggcgtagtt catcttcact
     1201 gatggtttcg tggcgggtta aggtacttcc cacgcacacc agacctgcgg cgacatccag
     1261 cgccgacgca atgaccccgc cgtgcaaaat gctttgtgcc cagttaccca ccatcatcgg
     1321 ctgattttta aaggccagct gtgcgaactc tttttcgtaa cgctccagtt ccatccccaa
     1381 tgcgcggtta aatggcatgt gataaacaaa catctcaccc actaatttca gggcttgttc
     1441 agcggtcagt acggcagaca tatcatcctt acacttcatt ggttaatgaa atgttgattt
     1501 tatgcttctt tgttgttggt ttctacttta ggaagggata actaacggca atggagttaa
     1561 gtatgtaaaa tagcccgcag aaaaacattc accttatcaa taattcgtta cggagaacac
     1621 gaccgatgcg gactctgcag ggctggttgt tgccggtgtt tatgttgcct atggcagtat
     1681 atgcacaaga ggcaacggtg aaagaggtgc atgacgcgcc agcggtgcgt ggcagtatta
     1741 tcgccaatat gctgcaggag catgacaatc cgttcacgct ctatccttat gacaccaact
     1801 acctcatcta cacccaaacc agcgatctga acaaagaagc gattgccagt tacgactggg
     1861 cggaaaatgc gcgtaaggat gaagtaaagt ttcagttgag cctggcattt ccactgtggc
     1921 gtgggatttt aggcccgaac tcggtgttgg gtgcgtctta tacgcaaaaa tcctggtggc
     1981 aactgtccaa tagcgaagag tcttcaccgt ttcgtgaaac caactacgaa ccgcaattgt
     2041 tcctcggttt tgccaccgat taccgttttg caggttggac gctgcgcgat gtggagatgg
     2101 ggtataacca cgactctaac gggcgttcag acccgacctc ccgcagctgg aaccgccttt
     2161 atactcgcct gatggcagaa aacggtaact gtctggtaga agtgaagccg tggtatgtgg
     2221 tgggtaatac tgacgataac ccggatatca ccaaatatat gggttactac cagcttaaaa
     2281 tcggctatca cctcggtgat gcggtgctca gtgcgaaagg acagtacaac tggaacaccg
     2341 gctacggcgg cgcggagtta ggcttaagtt acccgatcac caaacatgtg cgcctttata
     2401 ctcaggttta cagcggctat ggcgaatcgc tcatcgacta taacttcaac cagacccgtg
     2461 tcggtgtggg ggttatgcta aacgatttgt tttgatgaac ggttgagtgg ttggcaaatc
     2521 tggaatccag catccaggat taccctctca gagactaaaa gcattgcagt ttctcgcgca
     2581 ggcgctgaaa atagcgcctg tttttatttc aggcaatcgg ggtgaatgtg gcgcaggcgg
     2641 aagtgttgaa tctggagtcc ggagctaaac aggttttaca agataccttt ggctaccaac
     2701 agtttcgccc cggccaggaa gaaattatcg acactgtgct ttccggtcgc gattgcctgg
     2761 tcgtcatgcc caccggtggc ggaaaatccc tttgctatca aattcctgcc ttattgctaa
     2821 acggccttac cgtggttgtt tcaccgctga tttcgttgat gaaagatcag gtggatcaac
     2881 tgcaagccaa cggcgtggcg gcggcgtgcc ttaactcgac gcaaacccgc gagcagcaac
     2941 ttgaagtgat gacaggctgt cgcaccgggc aaatccgcct gctttatatc gcgccggaac
     3001 gcctgatgct ggataacttt cttgagcatc tggcgcactg gaatccggtg ttattagccg
     3061 ttgatgaagc gcactgtatc tcccaatggg gccacgattt ccgcccggaa tatgccgcgc
     3121 tcggtcagtt gcgccagcgg ttcccgacgc tgccgtttat ggcgctgacc gccacagccg
     3181 acgacaccac gcgccaggat atcgtgcgcc tgctggggct gaacgatccg ctgattcaaa
     3241 tcagcagttt tgaccgtccg aatattcgct acatgctgat ggagaaattc aaaccgctcg
     3301 atcagttgat gcgctacgtg caggaacagc gcggtaagcc cggcattatc tactgcaaca
     3361 gccgggcgaa agtagaagac accgctgcgc gcctgcaaag caagggtatt agcgcggcgg
     3421 cctatcatgc cgggctggaa aataatgtcc gcgccgacgt gcaggagaaa ttccagcgcg
     3481 atgacctgca aattgtggtg gcgacggtgg cgttcggcat gggcatcaat aaaccaaacg
     3541 ttcgcttcgt ggtccacttt gatattccgc gcaatatcga atcctattat caggaaaccg
     3601 gtcgcgccgg gcgtgatggc ctgcctgcgg aagcgatgct gttttacgat ccggctgata
     3661 tggcgtggct gcgccgttgt ctggaagaga agccgcaggg gcagttgcag gatatcgagc
     3721 gccacaaact caatgcgatg ggcgcgtttg ccgaagcgca aacttgccgt cgtctggtat
     3781 tgctgaacta ttttggcgaa gggcgtcagg agccgtgcgg gaactgcgat atctgcctcg
     3841 atccgccgaa acagtacgac ggttcaaccg atgctcagat tgccctttcc accattggtc
     3901 gtgtgaatca gcggtttggg atgggttatg tggtggaagt gattcgtggt gctaataacc
     3961 agcgtatccg cgactatggt catgacaaac tgaaagtcta tggcatgggc cgtgataaaa
     4021 gccatgaaca ttgggtgagc gtgatccgcc agctgattca cctcggcctg gtgacgcaaa
     4081 atattgccca gcattctgcc ctacaactga cagaggccgc gcgcccggtg ctgcgcggcg
     4141 aatcctcttt gcaacttgcc gtgccgcgta tcgtggcgct caaaccgaaa gcgatgcaga
     4201 aatcgttcgg cggcaactat gatcgcaaac tgttcgccaa attacgcaaa ctgcgtaaat
     4261 cgattgccga tgaaagcaat gtcccgccgt acgtggtgtt taacgacgca accttgattg
     4321 agatggctga acagatgccg atcaccgcca gcgaaatgct cagcgttaac ggcgttggga
     4381 tgcgcaagct ggaacgcttt ggtaaaccgt ttatggcgct tatccgcgcg catgttgacg
     4441 gcgacgacga agagtagtca gcggcataaa aaagtgccag gatgaagact ccgtaaacgt
     4501 ttcccccgcg agtcaaatgt atgttgatgt tatttctcac cgtcgccatg gtgcatattg
     4561 tggcgcttat gagccccggt cccgatttct tttttgtctc tcagaccgct gtcagtcgtt
     4621 cccgtaaaga agcgatgatg ggcgtgctgg gcattacctg cggtgtaatg gtttgggctg
     4681 ggattgcgtt gcttggcctg catttgatta tcgaaaaaat ggcctggctg catacgctga
     4741 ttatggttgg cggtggcctg tatctctgct ggatgggtta ccagatgcta cgcggtgcac
     4801 tgaaaaaaga ggctgtatct gcgcctgcac cacagattga gctggcgaaa agcgggcgca
     4861 gtttcctgaa aggtttactg accaatctcg ctaatccgaa agcgattatc tactttggct
     4921 cggtgttctc attgtttgtc ggtgataacg ttggcactac cgcgcgctgg ggcatttttg
     4981 cgctgatcat tgtcgaaaca ctggcgtggt ttaccgtcgt tgccagcctg tttgccctgc
     5041 cgcaaatgcg ccgtggttat caacgtctgg cgaagtggat tgatggtttt gccggggcgt
     5101 tatttgccgg atttggcatt catttgatta tttcgcggtg atgccagacg cgtcttcaga
     5161 gtaagtcgga taaggcgttt acgccgcatc cgacattatt tttcacgcat gcctcgccga
     5221 tgctaacagc gctcccacca gcataaacaa cgagccgaaa atcttattca gcgccttcat
     5281 ctgctttggt cctttaatcc atagagcaat ccgttgagca agggtggcgt aaccgatcat
     5341 cacaataata tcgactacaa tagtggtgac gccgagcacg atatactgca tcagttgcgg
     5401 ctgctgtggc atgatgaatt gcggaaatag cgccgccaga aacacaatac ttttgggatt
     5461 ggtgagattc acaaaaactg cgcgctggaa caaatgtcga cgcgattgag tagaggccag
     5521 cgatttaagg tcaattgcac cagcggcgcg ccactgctgg attcccagcc aaatcaagta
     5581 agccgcgcct gcccacttca acacttcaaa cgcaatcact gagcgggaaa atagcgtccc
     5641 caaccccacg ccaaccagca caatatgaat cgccagtccg gtctgaagcc cagcaataga
     5701 cgccaccgcg ccgcgataac cgtggttgag cgaggtggtc atagtgttga ttgcaccaga
     5761 gcctggcgac aggcttaaaa tgatcgatgt cagcaggtag gcaaaccacc attctaaggt
     5821 catgatgaac tcccggtgtg tctatttttg tgccacaata cgctactgtc gcagcgttgt
     5881 gtcaggcacg ctaaaaaaaa cgattttacg tggtttaaga ggcagattac ccgatgtttc
     5941 agcagcaaaa agactgggaa acaagagaaa acgcgtttgc tgcttttacc ctgggaccgc
     6001 tgactgattt ctggcgtcag cgtgatgaag cagagtttac tggtgtggat gacattccgg
     6061 tgcgctttgt ccgttttcgc gcacagcacc atgaccgggt ggtagtcatc tgcccggggc
     6121 gtattgagag ctacgtaaaa tatgcggaac tggcctacga cctgttccat ttggggtttg
     6181 atgtcttaat catcgaccat cgcgggcagg gacgttccgg tcgcctgtta gccgatccgc
     6241 atctcgggca tgttaatcgc tttaatgatt atgttgatga tctggcggca ttctggcagc
     6301 aggaggttca gcccggtccg tggcgtaaac gctatatact ggcacattcg atgggcggtg
     6361 cgatctccac attatttctg caacgccatc caggtgtatg tgacgccatt gcgctaactg
     6421 cgccaatgtt tgggatcgtg attcgtatgc cgtcatttat ggcacggcag atcctcaact
     6481 gggccgaagc gcatccacgt ttccgtgatg gctatgcaat aggcaccggg cgctggcgcg
     6541 cgttgccgtt tgctatcaac gtactgaccc acagcagaca gcgatatcga cgtaacttac
     6601 gcttctatgc tgatgaccca acgattcgcg tcggtgggcc gacctaccat tgggtacgcg
     6661 aaagtattct ggctggcgaa caggtgttag ccggtgcggg tgatgacgcc acgccaacgc
     6721 ttctcttgca ggctgaagag gaacgcgtgg tggataaccg catgcatgac cgtttttgtg
     6781 aactccgcac cgccgcgggc catcctgtcg aaggaggacg gccgttggta attaaaggtg
     6841 cttaccatga gatccttttt gaaaaggacg caatgcgctc agtcgcgctc cacgccatcg
     6901 ttgatttttt caacaggcat aactcaccca gcggaaaccg ctctacagag gtttaaattt
     6961 cttatgtacc aggttgttgc gtctgattta gatggtacgt tactttctcc cgaccatacg
     7021 ttatcccctt acgccaaaga aactctgaag ctgctcaccg cgcgcggcat caactttgtg
     7081 tttgcgaccg gtcgtcacca cgttgatgtg gggcaaattc gcgataatct ggagattaag
     7141 tcttacatga ttacctccaa tggtgcgcgc gttcacgatc tggatggtaa tctgattttt
     7201 gctcataacc tggatcgcga cattgccagc gatctgtttg gcgtagtcaa cgacaatccg
     7261 gacatcatta ctaacgttta tcgcgacgac gaatggttta tgaatcgcca tcgcccggaa
     7321 gagatgcgct tttttaaaga agcggtgttc caatatgcgc tgtatgagcc tggattactg
     7381 gagccggaag gcgtcagcaa agtgttcttc acctgcgatt cccatgaaca actgctgccg
     7441 ctggagcagg cgattaacgc tcgttggggc gatcgcgtca acgtcagttt ctctacctta
     7501 acctgtctgg aagtgatggc gggcggcgtt tcaaaaggcc atgcgctgga agcggtggcg
     7561 aagaaactgg gctacagcct gaaggattgt attgcgtttg gtgacgggat gaacgacgcc
     7621 gaaatgctgt cgatggcggg gaaaggctgc attatgggca gtgcgcacca gcgtctgaaa
     7681 gaccttcatc ccgagctgga agtgattggt actaatgccg acgacgcggt gccgcattat
     7741 ctgcgtaaac tctatttatc gtaatcgttc tttatttggt cagttgtcaa cctgatactt
     7801 cgctacaatg gatacccgtt aatcaaagag ttttccattg tggcgctact tatcatcacc
     7861 acgattctgt gggccttctc ctttagcttt tatggcgagt accttgcggg gcacgtcgat
     7921 agctattttg cggtgctggt gcgcgttggc ctggcggcac tcgtttttct gccgtttctg
     7981 cgtacccgtg gcaatagcct gaaaacagtc ggcctgtata tgctggtggg cgcgatgcag
     8041 ctcggcgtga tgtatatgct gagtttccgc gcttatctct acctgacggt ttccgaactg
     8101 ctgctgttca ccgtactgac gccgctctac atcacgctga tttatgacat catgagtaag
     8161 cgccgtctgc gctggggata tgcttttagt gccttgttgg cggtgattgg tgccgggatt
     8221 attcgctatg atcaggtcac cgaccatttc tggactggct tgttgctggt gcaactctcc
     8281 aatatcactt ttgccatcgg catggtgggt tacaaacgcc tgatggaaac tcgcccgatg
     8341 ccgcagcata acgcctttgc gtggttctat cttggcgcgt tcctggtggc ggtaattgca
     8401 tggttcttgc tgggaaatac gcagaaaatg ccgcaaacca cgttgcaatg gggcattctg
     8461 gtgtttcttg gcgtggtggc ttccgggatt ggctacttta tgtggaacta cggcgcgacg
     8521 caggtggacg ccggaacgct gggcattatg aataatatgc acgttccggc agggctgctg
     8581 gtaaacctgg ctatctggca ccaacagccg cactggccaa cgttcattac aggcgcgctg
     8641 gtgatcctgg cctcactgtg ggtgcatcgt aagtgggtcg ctccgcgctc ttcacaaacg
     8701 gcagatgatc gcaggcgtga ttgcgcgctg agcgaataaa cgcttccgta actggctgac
     8761 gctgctctcc atcgcgcacg gcggcgtaca gtcggctcca caagccttcg cccagggttt
     8821 tggtcaccac cagaccctgg cgctcaaaac tctctactac ccaatgcggt agcgcggcaa
     8881 tacccatccg tgcggcaacc atctgaatca acaataaggt gttatcgacg cttttcagtg
     8941 acgggctgac gcctgccggc tgaagaaaat gccgccagac atccagtcga ctacgctgta
     9001 ccggataaat caacagcgtc tcgctggcga gatcttccgg tgtaattcgc gttttcgccg
     9061 ccagtggatg gtcaggtgct aacaccagac gcacttcata gtcgaacatc ggcgaataat
     9121 gcaggccact gcgcggcagg atatcggagg tcatcaccag atccagctcg ccctgttgca
     9181 acgcgggctg cgggtcaaac gtcacgcccg atttaaaatc catctctacc tgcggccagt
     9241 tcttatggaa attttctaac gcgggcgtca gccactgaat acagctatgg cactcaatgg
     9301 caatgcgcag acgcgtctgc tgcggttcat tgcaggcttg cagtgcctgg ctgatttgcg
     9361 gcagcacctg gtttgccagt tgcaacagga tttctccctg cggtgtaaag cgtagcggct
     9421 ggctcttacg cacaaatagc cggaagccaa ggcgttgttc cagatcgcta aactggtgag
     9481 acagggcgga ttgcgtctga tgcaacgtcg ccgcagcggc tgcgagagag ccgcagttcc
     9541 gcaacgcttg tagcgttttc aggtgtttta cttcgatcat gaaagtcctt cacttcggca
     9601 tgaataattt gcgcttgagg aatatacagt aaccgccaat tatggatgtg taaacatctg
     9661 gacggctaaa tactaaaaat tccataaggg gcatataatg acaattctta atcacaccct
     9721 cggtttccct cgcgttggcc tgcgtcgcga gctgaaaaaa gcgcaagaaa gttattgggc
     9781 ggggaactcc acgcgtgaag aactgctgac agtagggcgt gaactgcgtg ctcgtcactg
     9841 ggatcaacaa aagcaagcgg gtatcgacct gctgccggtg ggcgattttg cctggtacga
     9901 tcatgtactg accaccagtt tgctgctggg taatgttccg ccacgtcatc agaacaaaga
     9961 cggttcggta gatatcgaca ccctgttccg tattggtcgt ggacgtgcgc cgactggcga
    10021 acctgcggcg gcagcggaaa tgaccaaatg gtttaacacc aactatcact acatggtgcc
    10081 ggagttcgtt aaaggccaac agttcaaact gacctggacg cagctgctgg aggaagtgga
    10141 cgaggcgctg gcgctgggcc acaacgttaa gcctgtgctg ctggggccgg ttacttacct
    10201 gtggctgggg aaagtgaaag gtgaacagtt tgaccgcctt agcctgctga acgacattct
    10261 gccggtttat cagcaagtgc tggcagaact ggcgaaacgc ggcatcgagt gggtacagat
    10321 tgatgaaccc gcgctggtac tggaactacc acaggcgtgg ctggacgcat acaaacccgc
    10381 ttacgacgcg ctccagggac aggtgaaact gctgctgacc acctattttg aaggcgtaac
    10441 gccaaatctc gacacgatta ctgcgctgcc tgttcagggt ctgcatgttg acctcgtaca
    10501 tggtaaagat gacgttgctg aactgcacaa gcgcctgcct tctgactggt tgctgtctgc
    10561 gggtctgatc aatggtcgta acgtctggcg cgccgatctt accgagaaat atgcgcaaat
    10621 taaggacatt gtcggcaaac gtgatttgtg ggtggcatct tcctgctcgt tgctgcacag
    10681 ccccatcgac ctgagcgtgg aaacgcgtct tgatgcagaa gtgaaaagct ggtttgcctt
    10741 cgccctacaa aaatgccatg aactggcact gctgcgcgat gcgctgaaca gtggtgacac
    10801 ggcagctctg gcagagtgga gcgccccgat tcaggcacgt cgtcactcta cccgcgtaca
    10861 taatccggcg gtagaaaagc gtctggcggc gatcaccgcc caggacagcc agcgtgcgaa
    10921 tgtctatgaa gtgcgtgctg aagcccagcg tgcgcgtttt aaactgccag cgtggccgac
    10981 caccacgatt ggttccttcc cgcaaaccac ggaaattcgt accctgcgtc tggatttcaa
    11041 aaagggcaat ctcgacgcca acaactaccg cacgggcatt gcggaacata tcaagcaggc
    11101 cattgttgag caggaacgtt tgggactgga tgtgctggta catggcgagg ccgagcgtaa
    11161 tgacatggtg gaatactttg gcgagcacct cgacggattt gtctttacgc aaaacggttg
    11221 ggtacagagc tacggttccc gctgcgtgaa gccaccgatt gtcattggtg acattagccg
    11281 cccggcaccg attaccgtgg agtgggcgaa gtatgcgcaa tcgctgaccg acaaaccggt
    11341 gaaagggatg ctgacggggc cggtgaccat actctgctgg tcgttcccgc gtgaagatgt
    11401 cagccgtgaa accatcgcca aacagattgc gctggcgctg cgtgatgaag tggccgatct
    11461 ggaagccgct ggaattggca tcatccagat tgacgaaccg gcgctgcgcg aaggtttacc
    11521 gctgcgtcgt agcgactggg atgcgtatct ccagtggggc gtagaggcct tccgtatcaa
    11581 cgccgccgtg gcgaaagatg acacacaaat ccacactcac atgtgttatt gcgagttcaa
    11641 cgacatcatg gattcgattg cggcgctgga cgcagacgtc atcaccatcg aaacctcgcg
    11701 ttccgacatg gagttgctgg agtcgtttga agagtttgat tatccaaatg aaatcggtcc
    11761 tggcgtctat gacattcact cgccaaacgt accgagcgtg gaatggattg aagccttgct
    11821 gaagaaagcg gcaaaacgca ttccggcaga gcgcctgtgg gtcaacccgg actgtggcct
    11881 gaaaacgcgc ggctggccag aaacccgcgc ggcactggcg aacatggtgc aggcggcgca
    11941 gaacttgcgt cgggggtaaa atccaaaccg ggtggtaata ccacccggtc ttttctcatt
    12001 acagcgactt cttcccacca tactgcttaa accattccag catacgctgc cagccatctt
    12061 ctgcagatgc ggcatgatag ctcgggcgat aatcagcgtt gaatgcatgc ccggcgtccg
    12121 ggtacacgat aatctctgct ttcgcattag cagcccgcag cgcctggcgc atggtttcaa
    12181 cgctctcctg cggaatgctg ttatcctgac caccatataa gccgagaatc ggcgcgttaa
    12241 gatcggttgc gatatcaaca ggttgtttcg gtgaattcag cgacttgtcg cccgtcagtt
    12301 tgccgtacca cgccactgcg gcttttagct gtggattatg cgcggcatac agccaggtga
    12361 tacgtccacc ccagcagaat ccggtgatca ttaaacgatg aacatcgccg ccgttgcggg
    12421 aagcccaact ggcgacatga tcgagatcgg ccagcacctg cgagtcaggc acttttgcta
    12481 ccagaccgct aagcaacgtg gggatatcgg caaaatcatt cggatcgcct tcgcggaagt
    12541 aaagttcagg tgcgatagcc agatatccct ccagcgccag acggcgacaa atgtcgcgga
    12601 tatgttcatg cacgccaaaa atttcctgca ctacaatgac cactggcagt gggccatcgc
    12661 tttgctttgg tctggcatgg taagcaggca tgttatcccc ttgtgaaggg atagaggtga
    12721 agcccgccac aatcgcgtcg tccggggtct gaacgatggt cgaagcgaga ggcgatgcag
    12781 caggtgcaaa tccagattgt tgtgttgttg ccatggtatt ctccgtaccc ttataaaaat
    12841 gttgcgcaat gttaactata gtcagcatgc aacaaatcac attgcctgaa tcggctcatc
    12901 ttttatgcag tcctgcagaa tgaagggtga tttatgtgat ttgcatcact tttggtgggt
    12961 aaatttatgc aacgcatttg cgtcatggtg atgagtatca cgaaaaaatg ttaaaccctt
    13021 cggtaaagtg tctttttgct tcttctgact aaaccgattc acagaggagt tgtatatgtc
    13081 caagtctgat gtttttcatc tcggcctcac taaaaacgat ttacaagggg ctacgcttgc
    13141 catcgtccct ggcgacccgg atcgtgtgga aaagatcgcc gcgctgatgg ataagccggt
    13201 taagctggca tctcaccgcg aattcactac ctggcgtgca gagctggatg gtaaacctgt
    13261 tatcgtctgc tctaccggta tcggcggccc gtctacctct attgctgttg aagagctggc
    13321 acagctgggc attcgcacct tcctgcgtat cggtacaacg ggcgctattc agccgcatat
    13381 taatgtgggt gatgtcctgg ttaccacggc gtctgtccgt ctggatggcg cgagcctgca
    13441 cttcgcaccg ctggaattcc cggctgtcgc tgatttcgaa tgtacgactg cgctggttga
    13501 agctgcgaaa tccattggcg cgacaactca cgttggcgtg acagcttctt ctgatacctt
    13561 ctacccaggt caggaacgtt acgatactta ctctggtcgc gtagttcgtc actttaaagg
    13621 ttctatggaa gagtggcagg cgatgggcgt aatgaactat gaaatggaat ctgcaaccct
    13681 gctgaccatg tgtgcaagtc agggcctgcg tgccggtatg gtagcgggtg ttatcgttaa
    13741 ccgcacccag caagagatcc cgaatgctga gacgatgaaa caaaccgaaa gccatgcggt
    13801 gaaaatcgtg gtggaagcgg cgcgtcgtct gctgtaattc tcttctcctg tctgaaggcc
    13861 gacgcgttcg gccttttgta tttttgcgta gcgcctcgca ggaaatgcct ttccaactgg
    13921 acgtttgtac agcacaattc tattttgtgc gggtaagttg ttgcgtcagg aggcgttgtg
    13981 gatttctcaa tcatggttta cgcagttatt gcgttggtgg gtgtggcaat tggctggctg
    14041 tttgccagtt atcaacatgc gcagcaaaaa gccgagcaat tagctgaacg tgaagagatg
    14101 gtcgcggagt taagcgcggc aaaacaacaa attacccaaa gcgagcactg gcgtgcagag
    14161 tgcgagttac tcaataacga agtgcgcagc ctgcaaagta ttaacacctc tctggaggcc
    14221 gatctgcgtg aagtaaccac gcggatggaa gccgcacagc aacatgctga cgataaaatt
    14281 cgccagatga ttaacagcga gcagcgcctc agtgagcagt ttgaaaacct cgccaaccgt
    14341 atttttgagc acagcaatcg ccgggttgat gagcaaaacc gtcagagtct gaacagcctg
    14401 ttgtcgccgc tacgtgaaca actggacggt ttccgccgtc aggttcagga cagcttcggt
    14461 aaagaagcac aagaacgcca taccctgacc cacgaaattc gcaatctcca gcaactcaac
    14521 gcgcaaatgg cccaggaagc gatcaacctg acgcgcgcgc tgaaaggcga caataaaacc
    14581 cagggcaact ggggcgaggt agtattgacg cgggtgctgg aggcttccgg tctgcgtgaa
    14641 gggtatgaat atgaaaccca ggtcagcatc gaaaatgacg cccgctcgcg gatgcagccg
    14701 gatgtcatcg tgcgcctgcc gcagggaaaa gatgtggtga tcgacgccaa aatgacgctg
    14761 gtcgcctatg aacgctattt taacgccgaa gacgactaca cccgcgaaag cgcgctacag
    14821 gaacatatcg cgtcggtgcg taaccatatc cgtttgctgg gacgcaaaga ttatcaacag
    14881 ctgccggggc tgcgaactct ggattacgtg ctgatgttta ttcccgttga acccgctttt
    14941 ttactggcgc ttgaccgcca gccggagctg atcaccgaag cgttgaaaaa caacatcatg
    15001 ctggttagcc cgactacgct gctggtggcg ctgcgcacta tcgccaacct gtggcgttat
    15061 gagcatcaaa gccgcaacgc ccagcaaatc gccgatcgtg ccagcaagct gtacgacaag
    15121 atgcgtttgt tcatcgatga catgtccgcg attggtcaaa gtctcgacaa agcgcaggat
    15181 aattatcggc aggcaatgaa aaaactctct tcagggcgcg gaaatgtgct ggcgcaggca
    15241 gaagcgtttc gcggtttagg agtagaaatt aaacgcgaga ttaatccgga tttggctgaa
    15301 caggcggtga gccaggatga agagtatcga cttcggtcgg ttccggagca gccgaatgat
    15361 gaagcttatc aacgcgatga tgaatataat cagcagtcgc gctagcccat tgggagtagt
    15421 taagccgggt agaaatctag ggcatcgacg cccaatctgt tacacttctg gaacaatttt
    15481 ttgatgagca ggcattgaga tggtggataa gtcacaagaa acgacgcact ttggttttca
    15541 gaccgtcgcg aaggaacaaa aagcggatat ggtcgcccac gttttccatt ccgtggcatc
    15601 aaaatacgat gtcatgaatg atttgatgtc atttggtatt catcgtttgt ggaagcgatt
    15661 cacgattgat tgcagcggcg tacgccgtgg gcagaccgtg ctggatctgg ctggtggcac
    15721 cggcgacctg acagcgaaat tctcccgcct ggtcggagaa actggcaaag tggtccttgc
    15781 tgatatcaat gaatccatgc tcaaaatggg ccgcgagaag ctgcgtaata tcggtgtgat
    15841 tggcaacgtt gagtatgttc aggcgaacgc tgaggcgctg ccgttcccgg ataacacctt
    15901 tgattgcatc accatttcgt ttggtctgcg taacgtcacc gacaaagata aagcactgcg
    15961 ttcaatgtat cgcgtgctga aacccggcgg ccgcctgctg gtgcttgagt tctcgaagcc
    16021 aattatcgag ccgctgagca aagcctatga tgcatactcc ttccatgtgc tgccgcgtat
    16081 tggctcactg gtcgcgaacg acgccgacag ctaccgttat ctggcagaat ccatccgtat
    16141 gcatcccgat caggataccc tgaaagccat gatgcaggat gccggattcg aaagtgtcga
    16201 ctactacaat ctgacggcag gggttgtggc gctgcatcgt ggttataagt tctgacagga
    16261 gaccggaaat gccttttaaa cctttagtga cggcaggaat tgaaagtctg ctcaacacct
    16321 tcctgtatcg ctcacccgcg ctgaaaacgg cccgctcgcg tctgctgggt aaagtattgc
    16381 gcgtggaggt aaaaggcttt tcgacgtcat tgattctggt gttcagcgaa cgccaggttg
    16441 atgtactggg cgaatgggca ggcgatgctg actgcaccgt tatcgcctac gccagtgtgt
    16501 tgccgaaact tcgcgatcgc cagcagctta ccgcactgat tcgcagtggt gagctggaag
    16561 tgcagggcga tattcaggtg gtgcaaaact tcgttgcgct ggcagatctg gcagagttcg
    16621 accctgcgga actgctggcc ccttataccg gtgatatcgc cgctgaagga atcagcaaag
    16681 ccatgcgcgg aggcgcaaag ttcctgcatc acggcattaa gcgccagcaa cgttatgtgg
    16741 cggaagccat tactgaagag tggcgtatgg cacccggtcc gcttgaagtg gcctggtttg
    16801 cggaagagac ggctgccgtc gagcgtgctg ttgatgccct gaccaaacgg ctggaaaaac
    16861 tggaggctaa atgacgccag gtgaagtacg gcgcctatat ttcatcattc gcactttttt
    16921 aagctacgga cttgatgaac tgatccccaa aatgcgtatc accctgccgc tacggctatg
    16981 gcgatactca ttattctgga tgccaaatcg gcataaagac aaacttttag gtgagcgact
    17041 acgactggcc ctgcaagaac tggggccggt ttggatcaag ttcgggcaaa tgttatcaac
    17101 ccgccgcgat ctttttccac cgcatattgc cgatcagctg gcgttattgc aggacaaagt
    17161 tgctccgttt gatggcaagc tggcgaagca gcagattgaa gctgcaatgg gcggcttgcc
    17221 ggtagaagcg tggtttgacg attttgaaat caagccgctg gcttctgctt ctatcgccca
    17281 ggttcatacc gcgcgattga aatcgaatgg taaagaggtg gtgattaaag tcatccgccc
    17341 ggatattttg ccggttatta aagcggatct gaaacttatc taccgtctgg ctcgctgggt
    17401 gccgcgtttg ctgccggatg gtcgccgtct gcgcccaacc gaagtggtgc gcgagtacga
    17461 aaagacattg attgatgaac tgaatttgct gcgggaatct gccaacgcca ttcagcttcg
    17521 gcgcaatttt gaagacagcc cgatgctcta catcccggaa gtttaccctg actattgtag
    17581 tgaagggatg atggtgatgg agcgcattta cggcattccg gtgtctgatg ttgcggcgct
    17641 ggagaaaaac ggcactaaca tgaaattgct ggcggaacgc ggcgtgcagg tgttcttcac
    17701 tcaggtcttt cgcgacagct ttttccatgc cgatatgcac cctggcaaca tcttcgtaag
    17761 ctatgaacac ccggaaaacc cgaaatatat cggcattgat tgcgggattg ttggctcgct
    17821 aaacaaagaa gataaacgct atctggcaga aaactttatc gccttcttta atcgcgacta
    17881 tcgcaaagtg gcagagctac acgtcgattc tggctgggtg ccaccagata ccaacgttga
    17941 agagttcgaa tttgccattc gtacggtctg tgaacctatc tttgagaaac cgctggccga
    18001 aatttcgttt ggacatgtac tgttaaatct gtttaatacg gcgcgtcgct tcaatatgga
    18061 agtgcagccg caactggtgt tactccagaa aaccctgctc tacgtcgaag gggtaggacg
    18121 ccagctttat ccgcaactcg atttatggaa aacggcgaag cctttcctgg agtcgtggat
    18181 taaagatcag gtcggtattc ctgcgctggt gagagcattt aaagaaaaag cgccgttctg
    18241 ggtcgaaaaa atgccagaac tgcctgaatt ggtttacgac agtttgcgcc agggcaagta
    18301 tttacagcac agtgttgata agattgcccg cgagcttcag tcaaatcatg tacgtcaggg
    18361 acaatcgcgt tattttctcg gaattggcgc tacgttagta ttaagtggca cattcttgtt
    18421 ggtcagccga cctgaatggg ggctgatgcc cggctggtta atggcaggtg gtctgatcgc
    18481 ctggtttgtc ggttggcgca aaacacgctg attttttcat cgctcaaggc gggccgtgta
    18541 acgtataatg cggctttgtt taatcatcat ctaccacaga ggaacatgta tgggtggtat
    18601 cagtatttgg cagttattga ttattgccgt catcgttgta ctgctttttg gcaccaaaaa
    18661 gctcggctcc atcggttccg atcttggtgc gtcgatcaaa ggctttaaaa aagcaatgag
    18721 cgatgatgaa ccaaagcagg ataaaaccag tcaggatgct gattttactg cgaaaactat
    18781 cgccgataag caggcggata cgaatcagga acaggctaaa acagaagacg cgaagcgcca
    18841 cgataaagag caggtgtaat ccgtgtttga tatcggtttt agcgaactgc tattggtgtt
    18901 catcatcggc ctcgtcgttc tggggccgca acgactgcct gtggcggtaa aaacggtagc
    18961 gggctggatt cgcgcgttgc gttcactggc gacaacggtg cagaacgaac tgacccagga
    19021 gttaaaactc caggagtttc aggacagtct gaaaaaggtt gaaaaggcga gcctcactaa
    19081 cctgacgccc gaactgaaag cgtcgatgga tgaactacgc caggccgcgg agtcgatgaa
    19141 gcgttcctac gttgcaaacg atcctgaaaa ggcgagcgat gaagcgcaca ccatccataa
    19201 cccggtggtg aaagataatg aagctgcgca tgagggcgta acgcctgccg ctgcacaaac
    19261 gcaggccagt tcgccggaac agaagccaga aaccacgcca gagccggtgg taaaacctgc
    19321 tgcggacgct gaaccgaaaa ccgctgcacc ttccccttcg tcgagtgata aaccgtaaac
    19381 atgtctgtag aagatactca accgcttatc acgcatctga ttgagctgcg taagcgtctg
    19441 ctgaactgca ttatcgcggt gatcgtgata ttcctgtgtc tggtctattt cgccaatgac
    19501 atctatcacc tggtatccgc gccattgatc aagcagttgc cgcaaggttc aacgatgatc
    19561 gccaccgacg tggcctcgcc gttctttacg ccgatcaagc tgacctttat ggtgtcgctg
    19621 attctgtcag cgccggtgat tctctatcag gtgtgggcat ttatcgcccc agcgctgtat
    19681 aagcatgaac gtcgcctggt ggtgccgctg ctggtttcca gctctctgct gttttatatc
    19741 ggcatggcat tcgcctactt tgtggtcttt ccgctggcat ttggcttcct tgccaatacc
    19801 gcgccggaag gggtgcaggt atccaccgac atcgccagct atttaagctt cgttatggcg
    19861 ctgtttatgg cgtttggtgt ctcctttgaa gtgccggtag caattgtgct gctgtgctgg
    19921 atggggatta cctcgccaga agacttacgc aaaaaacgcc cgtatgtgct ggttggtgca
    19981 ttcgttgtcg ggatgttgct gacgccgccg gatgtcttct cgcaaacgct gttggcgatc
    20041 ccgatgtact gtctgtttga aatcggtgtc ttcttctcac gcttttacgt tggtaaaggg
    20101 cgaaatcggg aagaggaaaa cgacgctgaa gcagaaagcg aaaaaactga agaataaatt
    20161 caaccgcccg tcagggcggt tgtcatatgg agtacaggat gtttgatatc ggcgttaatt
    20221 tgaccagttc gcaatttgcg aaagaccgtg atgatgttgt agcgtgcgct tttgacgcgg
    20281 gagttaatgg gctactcatc accggcacta acctgcgtga aagccagcag gcgcaaaagc
    20341 tggcgcgtca gtattcgtcc tgttggtcaa cggcgggcgt acatcctcac gacagcagcc
    20401 agtggcaagc tgcgactgaa gaagcgatta ttgagctggc cgcgcagcca gaagtggtgg
    20461 cgattggtga atgtggtctc gactttaacc gcaacttttc gacgccggaa gagcaggaac
    20521 gcgcttttgt tgcccagcta cgcattgccg cagatttaaa catgccggta tttatgcact
    20581 gtcgcgatgc ccacgagcgg tttatgacat tgctggagcc gtggctggat aaactgcctg
    20641 gtgcggttct tcattgcttt accggcacac gcgaagagat gcaggcgtgc gtggcgcatg
    20701 gaatttatat cggcattacc ggttgggttt gcgatgaacg acgcggactg gagctgcggg
    20761 aacttttgcc gttgattccg gcggaaaaat tactgatcga aactgatgcg ccgtatctgc
    20821 tccctcgcga tctcacgcca aagccatcat cccggcgcaa cgagccagcc catctgcccc
    20881 atattttgca acgtattgcg cactggcgtg gagaagatgc cgcatggctg gctgccacca
    20941 cggatgctaa tgtcaaaaca ctgtttggga ttgcgtttta gagtttgcgg aactcggtat
    21001 tcttcacact gtgcttaatc tctttattaa taagattaag caatagcatg gagcgagcct
    21061 caccatcggg ttcggtgaaa atggcctgaa agccttcgaa cgcgccttcg gtaataatca
    21121 ccttatcacc cggataaggg gttgccggat cgacaatgtc tttcggttta tataccgata
    21181 gctgatgaat aaccgccgat gggactatcg ctggcgacgc gccaaagcgc acgaagtggc
    21241 tgacaccgcg ggtcgcgttg atagtcgtgg tatgaatcac ttctggatca aattccacaa
    21301 acaggtagtt ggggaacaat ggctcactga ctgcagtacg ttttccacgc acgatttttt
    21361 ccagggtgat catcggtgcc aggcaattca cagcctgtct ttcgaggtgt tcctgggcac
    21421 gttgaagttg cccgcgcttg cagtacagta aataccagga ttgcataatg actcttatcc
    21481 gtttaatcgg ggcgcaagga tagcaaaagc tttacgctaa gttaattata ttccccggtt
    21541 tgcgttatac cgtcagagtt cacgctaatt taacaaattt acagcatcgc aaagatgaac
    21601 gccgtataat gggcgtagat taagaggcta caatggacgc catgaaatat aacgatttac
    21661 gcgacttcct gacgctgctt gaacagcagg gtgagctaaa acgtatcacg ctcccggtgg
    21721 atccgcatct ggaaatcact gaaattgctg accgcacttt gcgtgccggt gggcctgcgc
    21781 tgttgttcga aaaccctaaa ggctactcaa tgccggtgct gtgcaacctg ttcggtacgc
    21841 caaagcgcgt ggcgatgggc atggggcagg aagatgtttc ggcgctgcgt gaagttggta
    21901 aattattggc gtttctgaaa gagccggagc cgccaaaagg tttccgcgac ctgtttgata
    21961 aactgccgca gtttaagcaa gtattgaaca tgccgacaaa gcggctgcgt ggtgcgccct
    22021 gccaacaaaa aatcgtctct ggcgatgacg tcgatctcaa tcgcattccc attatgacct
    22081 gctggtcgga agatgccgca ccgctgatta cctgggggct gacagtgacg cgcggcccgc
    22141 ataaagagcg gcagaatctg ggcatttatc gccagcagct gattggtaaa aacaaactga
    22201 ttatgcgctg gctgtcgcat cgcggcggcg cgctggatta tcaggagtgg tgtgcggcgc
    22261 atccgggcga acgtttcccg gtttctgtgg cgctgggtgc cgatcctgcc acgattctcg
    22321 gtgcagtcac ccccgttccg gatacgcttt cagagtatgc gtttgccgga ttgctacgcg
    22381 gcaccaaaac cgaagtagta aagtgtattt ccaatgacct cgaagtgcca gccagtgccg
    22441 aaatcgtcct ggaagggtat atcgatcctg gtgagatggc gccggaaggg ccgtatggtg
    22501 accacacagg ttactataat gaagtcgata gtttcccggt gtttaccgtg acgcatatta
    22561 cccagcgtga agatgcgatt taccattcca cctataccgg gcgtccgcca gatgagcccg
    22621 cggtactggg cgtggcgttg aacgaagtgt ttgtaccgat tctgcaaaaa cagttcccgg
    22681 aaattgtcga tttttacctg ccgccggaag gctgttctta tcgtctggcg gtagtgacga
    22741 tcaaaaaaca gtacgccgga cacgcgaagc gcgtcatgat gggcgtctgg tcgttcttac
    22801 gccagtttat gtacactaaa tttgtgatcg tttgcgatga tgacgtcaac gcacgcgact
    22861 ggaacgatgt gatttgggcg attaccaccc gtatggaccc ggcgcgggat actgttctgg
    22921 tagaaaatac gcctattgat tatctggatt ttgcctcgcc tgtctccggg ctgggttcaa
    22981 aaatggggct ggatgccacg aataaatggc cgggggaaac ccagcgtgaa tggggacgtc
    23041 ccatcaaaaa agatccagat gttgtcgcgc atattgacgc catctgggat gaactggcta
    23101 tttttaacaa cggtaaaagc gcctgatgcg cgtttgtttt gccctattta tcgatccgac
    23161 agagaaagcg catgacaacc ttaagctgta aagtgacctc ggtagaagct atcacggata
    23221 ccgtatatcg tgtccgcatc gtgccagacg cggccttttc ttttcgtgct ggtcagtatt
    23281 tgatggtagt gatggatgag cgcgacaaac gtccgttctc aatggcctcg acgccggatg
    23341 aaaaagggtt tatcgagctg catattggcg cttctgaaat caacctttac gcgaaagcag
    23401 tcatggaccg catcctcaaa gatcatcaaa tcgtggtcga tattccccac ggagaagcgt
    23461 ggctgcgcga tgatgaagag cgtccgatga ttttgattgc gggcggcacc gggttctctt
    23521 atgcccgctc gattttgctg acagcgttgg cgcgtaaccc aaaccgtgat atcaccattt
    23581 actggggcgg gcgtgaagag cagcatctgt atgatctctg cgagcttgag gcgctttcgt
    23641 taaagcaccc tggtctgcaa gtggtgccgg tggttgaaca accggaagca ggctggcgtg
    23701 ggcgtactgg taccgtgtta acggcggtat tgcaggatca tggtacgctg gcagagcatg
    23761 atatctatat tgccggacgt tttgagatgg cgaaaattgc ccgtgacctg ttttgcagtg
    23821 agcgtaatgc gcgggaagat cgcctgtttg gcgatgcgtt tgcatttatc tgagatataa
    23881 aaaaacccgc ccctgacagg cgggaagaac ggcaactaaa actttattca gtggcattta
    23941 gatctatgac gtatctggca aaccatgccc gatgcgacgc tgtcgcgtct tatcgtgcct
    24001 acaaatagtc cgaaccgtag gccggataag gcgtttacgc cgcatccggc aattggtgca
    24061 cgatgcctga tgcgacgctg gcgcgtctta tcaggcctac attggtgccg gatctgtaga
    24121 ccggataagg cgttcacgcc gcatccggca agtggttaaa cccgctcaaa caccgtcgca
    24181 ataccctgac ccagaccgat acacatcgtc gccagaccaa actgaacgtc tttgcgctcc
    24241 atcagattca gcagcgtggt gctgatacgc gcaccggaac aacccagcgg atgacccagc
    24301 gcgatcgcgc caccattgag gttgatcttc tcgtcaatct gctccattag cccgagatct
    24361 ttaatgcacg gcaggatctg cgcggcaaag gcttcgttca tctcaaacac gccgatatcg
    24421 ctggcagaaa gccccgcttt tttcagcgcc agctttgagg ccggaaccgg gccgtaaccc
    24481 ataatcgatg ggtcacaacc aacgaccgcc atcgaacgca cacgagcgcg cggcttaaga
    24541 cctaattcat gggcgcggct ttcactcatc accagcatgg cagctgcgcc atcggaaagt
    24601 gcagaagatg tgcccgccgt taccgtaccg tttactggat caaacgccgg acgcagcgtg
    24661 gcgagggctt ccacggtggt ttccgggcga atcacttcgt cgtaattaaa ctgcttcagg
    24721 acgccgtcgg catcgtgacc accggtcggg atgatttcat ttttaaatgc ggccgactgc
    24781 gtggcggccc aggcacgagc gtgcgatcgc gcggcaaagg catcctgcat ttcacggctg
    24841 ataccgtgca tacgcgccag catttctgct gttaagccca tcatgcccgc cgctttggcg
    24901 acattgcggc tcaggccggg gtgaaaatcg acgccgtgac tcatcggcac atggcccata
    24961 tgctccacgc cgccaaccag acatgcctgc gcatcgccgg tcataatcat ccgtgctgcg
    25021 tcatgcagtg cctgcatgga tgaaccacac aagcgattga cggtaaccgc cgggacagag
    25081 tgtggtactt ctgccagcag cgccgcgtta cgggcgatgt taaaaccctg ctccagcgtc
    25141 tgctgcacac aaccccagta aatatcgtcg agggccgccg cttccagctc cgggttacgc
    25201 gccagcaggc tacgcattaa atgagcggag agatcttctg cacgcacgtt acgaaaagca
    25261 ccgcccttcg aacggcccat cggggtgcga attgcatcga caatgacaac ctgttccatt
    25321 gtgactcctt aagccgtttt caggtcgcca accggacggg ctggctcaac cggaggatag
    25381 tacggttcgt tatgacgcgc tttattacgc agaccttccg gcacttcata cagcgggccg
    25441 aggtgctgat attgctgtgc catatcgagg tattttgcgc taccgagggt gtccagccag
    25501 cggaacgcgc cgccgtggaa cggagggaag cccaggccgt agaccagcgc catatccgct
    25561 tccgccggag tggcgataat gccttcctcc agacagcgca ccacttcgtt gaccatcggg
    25621 atcatcatgc gggcgataat ctcctcttcg ctgaaatcgc gcttcggctg gctcacttct
    25681 gccagcaggt cttcaacggc ggcgtcttct tctttcttcg gcttaccttt gctgtcttct
    25741 ttataacgcc agaaaccgag gccgttcttc tgaccaaagc ggttggcatc aaacagcgcg
    25801 tcgatggcat cgcggtaatc tttctgcatc cgctgcggga agcctgctgc catgacagcc
    25861 tgagcgtgat gcgcggtatc aatccccaca acgtccagca ggtatgccgg acccatcggc
    25921 cagccaaact gtttttccat cactttgtcg atcttgcgga agtctgcgcc gtcgcgcagc
    25981 aactggctga atccggcgaa gtaggggaac agtacacggt taacgaagaa gccggggcag
    26041 tcgttgacca caatcggcgt cttgcccatc ttgctcgccc atgcgacgac tttcgcgatg
    26101 gtttcgtcgg agcttttctc gccgcgaata atttctacca acggcatccg gtgcaccggg
    26161 ttaaagaagt gcatcccgca gaagttttcc ggacgttcca gcgcgttggc cagttcgctg
    26221 ataggaatgg ttgaagtgtt agacgccagc acggtatcct ggcgtacttt ttgttcggtt
    26281 tctgccagta cggctttttt cactttcggg ttttcaacaa ccgcttctac cacaacatcc
    26341 acgcggtcaa acccggcgta gtcgagtgtt gggtggattg tggagatcac gccagccagt
    26401 ttcagaccat cgatcttgcc gcgctcaagc tgcttgttca gcagtttcgc ggcttcggtc
    26461 atgccgaggg ttaacgactt gtcgttgata tctttcatga caaccggcac gcctttccac
    26521 gcagactggt aagcgatgcc gccgcccata atgcctgcgc ccagcacagc ggcctgtttc
    26581 ggggtttcaa cgtctttggt gagtttcttc gctttgcctt ttacatattg atcgttaagg
    26641 aatatgccga ccagtgcgcg agcttcgttg gtatgcgcca gcgggacaaa acttttgttt
    26701 tccaggttta gggcttcttc acgaccaaaa cgggccgcag cttcaatggt ttttactgcg
    26761 gtgatggggg ccggataatg tttccccgcc gtttgtgcga ccatcccttt agcgatggtg
    26821 aagctcatgg tggcttcaat cttgctcagt tttagcggtt ccagcttcgg ctgacgtttc
    26881 gctttccagt cgaggtcgcc gttaatggcc tggcgtaaaa tcgccatcgc cccttcaatc
    26941 agtttttctg ctttgactac gccatccacc agaccgattt tcagcgcctg atccgcgccg
    27001 acatctttac cggcggcaat gatttccagc gcactgtcag cgcccagcat acgtggcata
    27061 cgtacagaac cgccaaagcc cggcatgatg cccagtttgg tttccggcag accgatgcgc
    27121 agatccggcg tcgccagacg ataatcggtc gccagcacgc attcgcagcc accgcccagc
    27181 gcatagccat tgacggcagc aatggtcggc accggcagat cttccaggcg attaaacacg
    27241 ctattggcaa agtgcagcca ctgacttaac tgttcttcag gaacgaggaa cagggacaaa
    27301 aattcggtga tatcagcacc gacgataaag gctgctttgt tcgaacgtag cagcagccct
    27361 tttagatctg attgctgttc cagcacgccg atggcctcgc cgaggctggc gacggtcgca
    27421 gtgtcgagtt tattaactga acctggggca tcaaatacca gttcggcaat gccatcttcc
    27481 agccagtcaa ggtacagggt gtcgcctttg taaagcatgt cagtctcctg aatccgcaag
    27541 gtgatctggt cgtaccagat gagtcgaagt gtgtattttg tgttaaaaat atgcaaacaa
    27601 aagattaaag aaatgccgat ctgatcacgc tcggcagaaa tcacgctctg gatgaacgat
    27661 gtgctaagat gcggagactt aaggtcaaaa aaacagaagg gtaaaaaatg gaatcactgg
    27721 cctcgctcta taaaaatcat atagctacct tacaggaacg gactcgcgat gcgctggcgc
    27781 gcttcaagct ggatgcgtta cttattcact ccggcgagct gttcaatgtt tttctcgacg
    27841 atcatcccta tccgtttaaa gtgaacccgc aattcaaagc gtgggtgccg gtaactcagg
    27901 tgccaaactg ctggttgctg gtggatggcg tgaacaagcc gaaactgtgg ttctatctgc
    27961 cggttgatta ctggcacaac gtcgaaccgc tgccgacctc cttctggact gaagatgtgg
    28021 aagtgatcgc actgccgaaa gccgatggca ttggtagcct gctgcccgct gcgcgcggca
    28081 atatcggtta tatcggtccg gtgccggagc gtgcgctgca actgggtatt gaggccagca
    28141 acatcaaccc gaaaggggtt atcgactacc tgcattacta tcgctccttc aaaaccgagt
    28201 acgaactggc ctgtatgcgt gaagcgcaga aaatggcggt caacggtcat cgtgcggcag
    28261 aagaagcgtt ccgttctggc atgagcgagt tcgatatcaa cattgcctat ctgaccgcga
    28321 ccggtcatcg tgataccgac gtaccttaca gcaacattgt ggcgcttaac gaacacgctg
    28381 cggtgctgca ttacaccaaa ctggatcatc aggcgccgga agagatgcgc agcttcctgc
    28441 tggatgccgg ggccgaatat aacggctatg cggctgacct gacccgtacc tggtcggcaa
    28501 aaagtgacaa tgattacgca cagctggtga aagacgtaaa tgatgaacaa ctggcgctga
    28561 tcgcgaccat gaaagctggc gttagctatg tggattacca catccagttc catcagcgca
    28621 tcgccaaact gctgcgtaaa catcaaatca tcaccgatat gagtgaagaa gcgatggtcg
    28681 aaaacgatct caccggaccg tttatgccgc acggtatcgg ccatccgctg ggcctgcagg
    28741 tgcatgacgt cgccggtttt atgcaggatg atagcggtac gcacctcgcg gcaccggcaa
    28801 aatatccgta cctgcgctgc acccgtattc tccagccggg catggtgtta accatcgaac
    28861 cgggtatcta cttcattgaa tcgctactgg caccgtggcg tgaagggcag ttcagcaagc
    28921 acttcaactg gcagaaaatt gaagcactga aaccgttcgg cggcattcgt atcgaagaca
    28981 acgtggtgat ccacgaaaac aacgtggaaa acatgacccg ggatctgaaa ctggcgtgat
    29041 ggaaagctgg ttaattcctg cggcaccggt cacggtcgtt gaagagatca aaaagagccg
    29101 cttcataaca ctgttggcgc ataccgatgg cgttgaggcg gcgaaagcgt ttgttgaatc
    29161 ggtgcgggca gaacaccccg atgcccgcca ccattgcgtg gcgtgggtgg cgggtgcgcc
    29221 ggatgattct caacagctgg gtttctctga cgacggggag ccggcaggaa cggcaggtaa
    29281 accgatgctc gcccagctaa tgggcagcgg cgtcggggaa attaccgctg tggtagtgcg
    29341 ctactacggt ggcatattgc ttggcaccgg tgggttagtg aaagcgtatg gcggcggcgt
    29401 gaatcaggcg ctgcgccagc taacgaccca acgcaagacg ccattaaccg aatatacttt
    29461 gcaatgtgaa tatagtcagt taaccggcat tgaagcgttg ctggggcagt gtgacggcaa
    29521 aattatcaac agtgattatc aggcattcgt tctgctgcgg gtggcgcttc cggcggcgaa
    29581 agtggctgaa ttttccgcaa agctggcgga ttttagccgt ggttcattgc aattgttagc
    29641 gattgaagaa taatccccac ttcgttttgc agaactaagg aagcggcaga gatgcatttt
    29701 cgcgccatta cccgaatcgt tggactactg gtcatcttat tttcagggac catgattatc
    29761 cctgggctgg tagcactcat ctaccgggat ggagcgggcc gcgcttttac ccagaccttt
    29821 tttgtcgccc tcgccattgg ctctatgctg tggtggccga accgcaaaga gaagggcgaa
    29881 ctgaaatccc gtgaggggtt tctgatagtg gtgctgtttt ggaccgtgct gggtagtgtc
    29941 ggtgcgctcc cttttatctt ctcggaaagc ccgaacctca cgattaccga tgcgtttttt
    30001 gaatctttct ctggcctgac caccaccggg gccactacgc tggtggggct ggattcgctc
    30061 cctcatgcca tcctctttta tcgccagatg ctgcaatggt ttggcgggat ggggatcatc
    30121 gtgttagcgg ttgcgatact gcctatcctc ggcgtgggtg ggatgcagct ctatcgcgca
    30181 gaaatgcccg gcccgctgaa agataacaaa atgcgcccgc gaattgcgga aacggcgaaa
    30241 accctgtggt tgatttatgt cttgctgacc gtcgcctgtg cgctggcgtt gtggtttgcc
    30301 ggaatggatg cctttgatgc catcggccat agctttgcga ctatcgctat tggcggcttc
    30361 tcgacacatg atgccagtat cggttatttc gacagcccga ctattaacac tatcattgct
    30421 atcttcctgc tgatctccgg ctgtaactac ggtctgcact tttcactgtt aagtgggcgt
    30481 agtctgaagg tttattggcg cgatccggaa tttcgcatgt ttatcggcgt acagtttacg
    30541 ctggtggtta tttgtacact cgtactgtgg tttcataatg tctacagttc ggcgctgatg
    30601 acaattaacc aggcgttttt ccaggtggta tcgatggcga caaccgccgg gtttacgact
    30661 gacagcattg cccgctggcc gctctttttg ccggtactgc ttttatgttc agcttttatc
    30721 ggcggttgtg ccgggtcaac gggcggtggc ctgaaagtga tccgcatcct gctgctgttt
    30781 aagcagggga accgtgagct gaaacgactg gtgcatccga acgccgtcta tagcattaag
    30841 ctggggaatc gcgcactgcc ggaacgtatc ctcgaagccg tttggggatt tttctccgcc
    30901 tatgcattgg tgtttattgt cagtatgctg gcgattatcg ccacgggcgt ggatgacttt
    30961 tctgcttttg cctcggttgt tgcgacattg aataacctgg ggccggggct tggcgtggtt
    31021 gctgataact ttaccagtat gaacccggtg gctaaatgga tcctgattgc caacatgctg
    31081 tttggtcgtc tcgaggtctt tacattgctg gtgctcttta ccccgacttt ctggcgtgaa
    31141 tgatggagta atacgtgaaa acattaattc ttttctcaac aagggacgga caaacgcgcg
    31201 agattgcctc ctacttggct tcggaactga aagaactggg gatccaggcg gatgtcgcca
    31261 atgtgcaccg cattgaagaa ccacagtggg aaaactacga ccgtgtggtc attggtgctt
    31321 ctattcgcta tggtcactac cactctgcgt tccaggaatt tgtcaaaaag catgcgacgc
    31381 ggctgaattc gatgccgagc gccttttact ccgtaaacct ggtggcgcgt aaaccggaga
    31441 agcgcactcc acagaccaac agctacgcgc ggaagtttct gatgaactcg caatggcgtc
    31501 ccgatcgctg cgcggtcatt gccggggcgc tgcgttaccc acgttatcgc tggtacgacc
    31561 gttttatgat caagctgatt atgaagatgt caggcggtga aacggatacg cgcaaagaag
    31621 ttgtctatac cgattgggag caggtggcga atttcgcccg agaaatcgcc catttaaccg
    31681 acaaaccgac gctgaaataa gcacaaagaa taaaaaatgc gcggtcagaa aattatttta
    31741 aatttcctct tgtcaggccg gaataactcc ctataatgcg ccaccactga cacggaacaa
    31801 cggcaaacac gtcgccgggt cagcggggtt ctcctgagaa tctcaacaga gaaaagcaaa
    31861 aaatgcttga ctctgtagcg ggaaagggta ttatgcacac cccgcgccgc tgagaaaaag
    31921 cgaagcggca ctgctcttta acaatttatc agacaatctg tgtgggcact cgaagatacg
    31981 gattcttaac gtcgcaagac gaaaaatgaa taccaagtct ca
//
LOCUS       DAPHBJ010000031        30738 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.031, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000031 DAPHBJ010000000
VERSION     DAPHBJ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.031"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            225..1312
                     /gene="pdeL"
                     /locus_tag="RHZ76_001335"
                     /pseudo
     CDS             225..1312
                     /gene="pdeL"
                     /locus_tag="RHZ76_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
     gene            complement(1354..2285)
                     /locus_tag="RHZ76_001336"
                     /pseudo
     CDS             complement(1354..2285)
                     /locus_tag="RHZ76_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(2377..2874)
                     /locus_tag="RHZ76_001337"
     CDS             complement(2377..2874)
                     /locus_tag="RHZ76_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDV2811999.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGISGFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            3132..3737
                     /locus_tag="RHZ76_001338"
     CDS             3132..3737
                     /locus_tag="RHZ76_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDV2812000.1"
                     /translation="MSIKNLPADYLLAAQQVDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHMTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            3777..4640
                     /locus_tag="RHZ76_001339"
     CDS             3777..4640
                     /locus_tag="RHZ76_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDV2812001.1"
                     /translation="MWALTADADFLAQRGRGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     ASAVASMMGLGIGLTPSVDDYLTGLVLILFISGHPAEKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            4630..6177
                     /gene="fdrA"
                     /locus_tag="RHZ76_001340"
     CDS             4630..6177
                     /gene="fdrA"
                     /locus_tag="RHZ76_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDV2812002.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISARANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLKDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            6177..7595
                     /locus_tag="RHZ76_001341"
     CDS             6177..7595
                     /locus_tag="RHZ76_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDV2812003.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTAKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGAHDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            7645..7941
                     /locus_tag="RHZ76_001342"
     CDS             7645..7941
                     /locus_tag="RHZ76_001342"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812004.1"
                     /translation="MAQESVGLISVAHQAFVTIAGCGVNALSSLRMAREFVGLISVAH
                     QAFATTAGCGVNALSGLRVARESVGLISVAHQAFVTIAGCGVNALSGLRVAREL"
     gene            8017..8967
                     /locus_tag="RHZ76_001343"
     CDS             8017..8967
                     /locus_tag="RHZ76_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HDV2812005.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQCDELQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            8977..10359
                     /locus_tag="RHZ76_001344"
     CDS             8977..10359
                     /locus_tag="RHZ76_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDV2812006.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTTHCDAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGDKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELANRMVVQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            10736..11785
                     /gene="yahK"
                     /locus_tag="RHZ76_001345"
     CDS             10736..11785
                     /gene="yahK"
                     /locus_tag="RHZ76_001345"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HDV2812007.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKYAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            12028..12843
                     /locus_tag="RHZ76_001346"
     CDS             12028..12843
                     /locus_tag="RHZ76_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812008.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNQWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            13256..13501
                     /locus_tag="RHZ76_001347"
     CDS             13256..13501
                     /locus_tag="RHZ76_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812009.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGWGRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAVIRQIRLIT"
     gene            complement(13518..14189)
                     /locus_tag="RHZ76_001348"
     CDS             complement(13518..14189)
                     /locus_tag="RHZ76_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HDV2812010.1"
                     /translation="MMQLVHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            14336..14611
                     /locus_tag="RHZ76_001349"
     CDS             14336..14611
                     /locus_tag="RHZ76_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV2812011.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(14709..16295)
                     /gene="prpR"
                     /locus_tag="RHZ76_001350"
     CDS             complement(14709..16295)
                     /gene="prpR"
                     /locus_tag="RHZ76_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HDV2812012.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMQQGRFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLLHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFMQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            16534..17424
                     /gene="prpB"
                     /locus_tag="RHZ76_001351"
     CDS             16534..17424
                     /gene="prpB"
                     /locus_tag="RHZ76_001351"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HDV2812013.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDNLFARSQVK"
     gene            17864..19033
                     /gene="prpC"
                     /locus_tag="RHZ76_001352"
     CDS             17864..19033
                     /gene="prpC"
                     /locus_tag="RHZ76_001352"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HDV2812014.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAKHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRPFVALDKRQ"
     gene            19067..20518
                     /gene="prpD"
                     /locus_tag="RHZ76_001353"
     CDS             19067..20518
                     /gene="prpD"
                     /locus_tag="RHZ76_001353"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HDV2812015.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTLFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            20558..22444
                     /gene="prpE"
                     /locus_tag="RHZ76_001354"
     CDS             20558..22444
                     /gene="prpE"
                     /locus_tag="RHZ76_001354"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HDV2812016.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDGRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            22868..24127
                     /gene="codB"
                     /locus_tag="RHZ76_001355"
     CDS             22868..24127
                     /gene="codB"
                     /locus_tag="RHZ76_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDV2812017.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            24117..25400
                     /gene="codA"
                     /locus_tag="RHZ76_001356"
     CDS             24117..25400
                     /gene="codA"
                     /locus_tag="RHZ76_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HDV2812018.1"
                     /translation="MSNNTLQTIINARLPGEEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHHEGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(25737..26636)
                     /gene="cynR"
                     /locus_tag="RHZ76_001357"
     CDS             complement(25737..26636)
                     /gene="cynR"
                     /locus_tag="RHZ76_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414872.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HDV2812019.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPSITLQLQEMSQEKIEDMLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAVHEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALDKCAVVGGNESR"
     gene            26745..27404
                     /gene="cynT"
                     /locus_tag="RHZ76_001358"
     CDS             26745..27404
                     /gene="cynT"
                     /locus_tag="RHZ76_001358"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HDV2812020.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            27435..27905
                     /gene="cynS"
                     /locus_tag="RHZ76_001359"
     CDS             27435..27905
                     /gene="cynS"
                     /locus_tag="RHZ76_001359"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HDV2812021.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDSILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            27911..29092
                     /gene="cynX"
                     /locus_tag="RHZ76_001360"
     CDS             27911..29092
                     /gene="cynX"
                     /locus_tag="RHZ76_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HDV2812022.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMREFYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPMQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPVRFPQLWVKE
                     A"
     gene            complement(29195..29806)
                     /gene="lacA"
                     /locus_tag="RHZ76_001361"
     CDS             complement(29195..29806)
                     /gene="lacA"
                     /locus_tag="RHZ76_001361"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HDV2812023.1"
                     /translation="MNMPMTERIRAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPCRVIREINDRDKHYYFKDYKVESSV"
     gene            complement(29872..>30738)
                     /gene="lacY"
                     /locus_tag="RHZ76_001362"
     CDS             complement(29872..>30738)
                     /gene="lacY"
                     /locus_tag="RHZ76_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HDV2812024.1"
                     /translation="EKVSRRSNFEFGRARMFGCVGWALCASIVGIMFTINNQFVFWLG
                     SGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFRQPKLWFLSLYVIG
                     VSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIMFFAPLIINRIGGK
                     NALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCFKYITSQFEVRFSA
                     TIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGFTLISVFTLSGPGP
                     LSLLRRQVNEVA"
BASE COUNT         7447 a         7817 c         8276 g         7198 t
ORIGIN      
        1 attcttgcgt gattatctcc tgagtttgac ttgtgattac ctttttaagg tatttagcgt
       61 aactgttttt gagcgagcat cagaggtaaa gataatctta ttgatagtga tgtgggatgt
      121 tatacgtatg gcatcgctga tgtttatggt taccccttat gtgtgctcag gaatcgacag
      181 gtaatcactc atactgaaca gcgataaaag ataaaggtgt gttcatgaat tcatgtgatt
      241 ttcgtgtttt tctgcaagag ttcggtacaa cggttcattt gtcattgcct ggtagcgtat
      301 ccgagaaaga acgactgcta ctcaagctgc tgatgcaggg aatgtcagta acagaaatat
      361 cacagtacag aaatcgcagt gcaaagacaa tttcacatca aaagaaacag ctctttgaga
      421 aactggggat tcagagcgat attactttct ggcgcgatat tttctttcag tacaatccgg
      481 agatcatatc cgccacgggg agtaatagtc acagatatat taatgataat cactatcacc
      541 atatcgtcac gcctgaagcc atcagtctgg cgttggaaaa ccacgaattc aaaccgtgga
      601 tccaaccggt tttctgcgcg cagactgggg tactgacggg ctgtgaggtg cttgtccgct
      661 gggaacatcc acaaacggga attatcccac cggatcagtt tattcctctg gcggagtcat
      721 ccggtcttat tgtcataatg acccgccact gatgaaacag actgcggata ttctgatgcc
      781 ggtaaaacat ttgctgccgg ataatttcca tattggtatc aacgtctcgg cgggttgttt
      841 tttggccgcg ggatttgaaa aagaatgcct gaacctggtc aagaaattag gaaacgataa
      901 aatcaaactg gttctcgagc taacggaacg taaccctatt ccggtaacgc cagaagccag
      961 agcgatattt gacagccttc atcagcacaa cattaccttt gcgctggatg actttggtac
     1021 gggttatgcg acctatcgtt atctgcaggc gtttccggtc gattttatta aaattgacaa
     1081 gtcgtttgtg caaatggcga gtgtcgacga aatctccggt catattgtgg acaatattgt
     1141 cgaactggcg cgtaagcctg gtctgagtat cgtggcggaa ggggtagaaa cccaggagca
     1201 ggcggattta atgatcggta aaggcgttca ctttttgcag ggctatttgt actccccgcc
     1261 agttccgggt aataaattta tctctgaatg ggtaatgaaa gcaggtggtt gatgtaaacc
     1321 gctattcaca gcgcatcggg aggttggcag cgattaattc tcccgatgca gtaaatcctg
     1381 atagatcccc gttaatatgc catttggcgc aaattgtttt ttaatccact gcgtcacctg
     1441 acccgtagca gaatgctgag tcgccagtaa catgcgcgaa tcctggcgcg gattatggat
     1501 ttgtcgggta accagcaggg attgcgtcat cgcctcacgg accatgtaat ccggcaaaaa
     1561 accaatccct tcacctaaaa tctggcactg acatttggtg ttgaaatctg gcaccagaat
     1621 tgactcctgc ccgtgcagca accagcccac ttttttatta atcgtatgcg cggtgtcctc
     1681 caccataata ttagggtaga gacgcagttg gctttcggcg atgggttccg gcataaaggc
     1741 taacgggtga tccggggcga tagcaaaagc ccagcgaatc gcgccaattt cagtgtaatc
     1801 aataccgccg ccgtccagta gtgtatcggg tgcgccgatg gcgatattgg cctgattatt
     1861 aataatcgca tcccagacgc cgttatacac ttcggtcgtc acggtaatct ggcaggtagg
     1921 aaactgcttt ttcagcacct gtaataagcg cgcggtgtgt ttgggcgtat aaagcagctg
     1981 attaatacaa atgcgcaccc gtgcttctat gcctgcgaaa tagtatctat gctacgtttg
     2041 atggcgtgaa aatcattcag caggtcggta gctttgcgga aaaaataacg cccggactcg
     2101 gttaactcaa tgctgcgggt gttgcgagtg aacagcacca catccagccc cgtctccata
     2161 cgcttgatgg tgtagctaat ggcggaagtg gttaaaccca actcgtctgc ggctttactg
     2221 aagctgccaa aacgcgcagc ggtagtaaat gccaacagat tctcttcggt aaaaattgag
     2281 ttcattaaac cattctcgcc attatctact atgaatttct ccagctatga ctgtagaggt
     2341 atcggttaaa gatagataat catttttgaa taacttttaa tacccgtcgc gtttagtcgt
     2401 cgcttcgctt aatttttgtc caccatattc tgaaattatc cccacgcagt tacccgccag
     2461 aaaacccagt aatgtcaccg tccagttcat cccgctggca aaaaatagcg tcatgccgag
     2521 aaagcctccg ggaataaaag acagtaacca gaaacgccct tgccagacca ctactgcaga
     2581 gaaaggcacg gtagtaatca cgctcgccca gaacccggaa atcccactca ccgaggccag
     2641 ccaaccggca gccagcgcgg cgaaaaacgc ccacaccatc ccggaataat taaccgccag
     2701 acttctgata aaccccgatt taccgccgcc tgcggcataa aaactgcaaa aggcgacgaa
     2761 gccaatggtt cctaataatt cccatccctg agagagcccg acatgtgaag acactaactg
     2821 ccaaagccct gcacaaatac cgacggtgac acccgttgcc gtgagtccgt tcatcttgtt
     2881 cccctctggt tagacatgga cgaaagctac tcttacatgt gcagactata gagaaataaa
     2941 acctgattga gtgaaagggt atcgggtcaa agaaacaaat attgcactac cgcacactgc
     3001 gaaaagattg ttgaacatca ttcaacaaaa aggcgaaata cggcgaatta aatttaagcg
     3061 ggttctttta tcagattatc gttgctattc tcaattcatc gaaaacagcc acaaaaacca
     3121 ggggaatgtg aatgagtata aaaaatctac ctgccgacta tttattggct gcacaacagg
     3181 ttgatatcga taaagtaaaa acctgccttg cgctgggtgt cgatattaat acctgcgacc
     3241 gtcaggggaa aacggcaatt acgctggcaa gtttatatca gcaatatgct tgcgttcagg
     3301 cattaattga tgccggagcg gatattaata agcaagatca tacctgttta aatccttttt
     3361 taattagctg tctgaacgat gatttaacgc tactacgaat tattttaccg gctaaacctg
     3421 atcttaattg cgtaacccgt tttggcggtg tcggcctgac gcctgcctgt gaaaaaggcc
     3481 atttaagtat tgtaaaagag cttttggcgc atacggaaat taacgttaac cagaccaacc
     3541 atgtaggctg gacgccgcta ctggaagcga ttgtgcttaa tgatggcggt attaaacagc
     3601 aggcgattgt ccagctattg ctggaacacg gtgccagtcc gcacatgacg gataaatatg
     3661 gcaaaacgcc gctggaactg gcgcgggaac ggggctttga agagattgct cagttgctga
     3721 ttgccgcagg cgcataaccc gggaggctgg ctatcaacac accagaaaaa cggtgtgtgt
     3781 gggcgctaac tgcggatgca gattttctgg cgcagcgggg gcgaggacag gttgaacagg
     3841 tctttgccag agcggtaaat atcgcactcc cggcccgcca gcagttgctg acgctgcttt
     3901 gtgaagagta cgacaatgcg ccaaacagtt gtcggttggc actcactcac tttgatgatc
     3961 ttttccggca tggtgataag gttcagtttg acgatcaagg cattacggtt ggtcaacatc
     4021 ttcatataga gatgagtcgt tgtcggcgtt ggctgtcccc aaccttgcaa atgaccgctg
     4081 tgaattttca ccttatcgcc tggcaacagt ggcacgacat tattcatcag cacctggggg
     4141 aaaatgaaac cctgtttaat tatcgcggcg ataatccgtt ttatcaggcg ttaaataaag
     4201 aattacatat taaacgacgg gcagttattc aggccgtaaa cgataaacaa aatatcgcct
     4261 cagcggtcgc cagtatgatg gggttaggga ttggccttac gccatcagtc gatgattatc
     4321 tgaccggact ggtacttatt ttatttattt ccggacatcc ggcggaaaaa tacaaagagg
     4381 aattttatct cggtctgcaa cgcggcagaa ataataccac gttattaagt gccataacgc
     4441 tggaagccgc attacaacaa cgctgccggg aaaatattca tcgctttatt cacaacatta
     4501 tttatgacat ccctgggaac gcaactcagg caatagaaaa aattaaacat attggctcca
     4561 gttccggctg cgacatgctg tatggcatgg ccgatggttg tgcgctgagc caaacctacg
     4621 gagggaatta tgtcagttaa aatagtcatt aaaccgaata cctattttga ttctgtctcg
     4681 ctgatgtcta tctccgcgcg tgcaaataaa ctcgacggcg tcgagcaggc atttgtggcg
     4741 atggcgaccg aaatgaacaa aggcgtgctg aagaatttag gactgctgac gccggaactg
     4801 gagcaggcga aaaacggtga cctgatgatt gtcatcaatg gtaaatcggg tgcggacaac
     4861 gagcagttgc tggtggagat tgaagaactg ttcaacacca aagcgcaaag cggctcgcac
     4921 gaggcgcgtt acgccactat tgccagcgcc aaaaagcata tcccggaaag taacctggcg
     4981 gtgatttcgg tcaacggttt gtttgccgct cgcgaagcgc gtcaggcgct gcaaaacgat
     5041 ctcaacgtga tgctgttttc cgataacgtc tcagttgaag atgaactggc gctcaagcaa
     5101 ctggcccacg aaaaagggct gctgatgatg gggccagact gtggcacggc gattatcaac
     5161 ggcgcggcgc tctgttttgg taacgccgtg cgtcgcggca acatcggtat tgttggcgca
     5221 tccggcaccg gcagccagga gctgagcgtc cgcattcatg aatttggcgg cggcgtttcg
     5281 caactcatcg gcaccggcgg gcgcgacctg agcgagaaaa tcggcggcct gatgatgctc
     5341 gacgccatcg ggatgctgga aaacgatccg caaactgaaa ttatcgcgct gatttcaaaa
     5401 cctcccgcgc ctgcggtggc ccgcaaagtg ctggaacgcg cacgtgcctg ccgcaagccg
     5461 gtggtggtct gcttcctcgg tcgcgttgaa acgccggtgg atgagcaggg gctacagttt
     5521 gcccgcggca gcaaagaggc ggcgctaaaa gcggtgatgc tctccggcgt gaaacaggaa
     5581 aatctcgacc tgcatacgct taaccagccg ttgattgcgg atgtgcgtgc gcgtctgcaa
     5641 ccgcagcaga aatacattcg tggcctgttc tgcggcggca cgttgtgcga cgaaaccatg
     5701 ttcgcggtga tggaaaaaca tggcgatgtc tacagcaata tccagcccga tccggaattc
     5761 cgcctgaaag atatcaaccg cagcatcaaa cacaccttcc tcgactttgg tgatgacgac
     5821 ttcaccaacg gcaagccgca cccgatgatt gaccccacca accgcatcag tcgcttgatc
     5881 gaagaggcgc gcgatccaga agtggcggtg atcgtgatgg attttgtgct cggatttgga
     5941 tcgcatgaag atccggtcgg ctccaccatc gaggcgatca aagaagcgaa agcgatcgcc
     6001 gctgccgaag gacgcgagtt gatcattctc gcctatgtgc tgggtaccga tcttgatacg
     6061 ccatcgttag aacaacaaag ccagatgctg cttgatgccg gagtgattct ggcgagcagc
     6121 agcaccaata ccggattgct ggcgcgtgaa tttatctgca aaggggagga agcctgatga
     6181 gccagtcact gtttagccaa ccattgaacg ttattaacgt cggcatcgcc atgtttagcg
     6241 atgacctgaa aaagcagcat gtagaagtga ctcaactcga ctggacgccg ccggggcagg
     6301 gcaatatgca ggtggtgcag gcgctggata acattgccga ttcgccgctg gcggacaaaa
     6361 tcgccgccgc taaccagcag gcgctggagc gtattatcca gtcgcatccg gtgctgattg
     6421 gttttgatca ggcgattaac gtggtgccgg gcatgacggc gaaaaccatt cttcacgccg
     6481 ggccgccgat cacctgggaa aaaatgtgcg gcgcgatgaa aggcgcggtc accggagcgc
     6541 tggtgttcga aggactggcg aaagatctcg acgaggcggc tgaactggcg gcttccgggg
     6601 agatcacctt ctcgccgtgt cacgagcacg actgcgtggg atcgatggcg ggtgttacct
     6661 cggcctcgat gtttatgcac atcgtaaaaa acaaaaccta cggcaacatc gcttatacca
     6721 acatgagcga gcagatggcg aagattttgc gtatgggcgc tcacgaccag agcgtgattg
     6781 accgcctgaa ctggatgcgt gatgtgcagg gaccaatact gcgcgacgcg atgaaaatta
     6841 tcggcgaaat cgatctgcgc ttaatgctgg cgcaggcgct gcatatgggc gatgagtgcc
     6901 ataaccgcaa taacgccggg acgacgctgc tgattcaggc gctgacgccg gggattattc
     6961 aggcgggtta ttccgtcgag caacagcgcg aagtgtttga gtttgtcgcc agcagcgact
     7021 acttctccgg cccgacgtgg atggcgatgt gtaaagcggc gatggatgcg gcgcatggca
     7081 tcgaatacag caccgtggtc accaccatgg cgcgtaacgg cgtcgagttc ggcctgcggg
     7141 tcagcggcct gccggggcaa tggtttaccg gcccggcgca gcaggtgatc ggcccgatgt
     7201 ttgccggtta taagccggaa gattcggggc tggatatcgg cgacagcgcc atcaccgaaa
     7261 cctacggtat tggcggattt gctatggcga ccgcgcccgc tatcgtcgcg ctggtgggcg
     7321 gcacggtgga agaagctatt gatttctccc gtcagatgcg cgaaatcacc ctcggtgaaa
     7381 accccaacgt caccattccg ctgctcggtt ttatgggcgt gccgtcggca atcgacatca
     7441 cccgcgtggg tagcagcggc attctgccgg tgatcaacac cgccatcgcc cataaagatg
     7501 cgggcgtcgg catgattggc gcgggcattg tgcatccacc ttttgcctgc ttcgagaaag
     7561 ccattcttgg ctggtgcgaa cgttacggcg tctgacctac atgttcatgc cggatgcggc
     7621 gtgaacgcct tatccggcct acgaatggcg caagaatctg taggcctgat aagcgtagcg
     7681 catcaggcat ttgtcaccat tgccggatgc ggcgtgaacg ccttatccag cctacggatg
     7741 gcgcgagaat ttgtaggcct gataagcgta gcgcatcagg catttgccac cactgccgga
     7801 tgcggcgtga acgccttatc cggcctacga gtggcgcgag aatctgtagg cctgataagc
     7861 gtagcgcatc aggcatttgt caccattgcc ggatgcggcg tgaacgcctt atccggccta
     7921 cgagtggcgc gagaattgta ggcctgataa gcgtagcgca tcaggcagtc tggcgttggt
     7981 cataacccca tcaccctctg tagcggacat aacaacatga aagagcttgt ggtcgttgcc
     8041 attggtggca acagcattat caaagataac gccagccagt cgattgagca tcaggcggag
     8101 gcggtgaaag ccgtcgccga tacggtgctg gaaatgctgg cttccgatta cgacattgtg
     8161 ctgacccacg gcaacgggcc gcaggtcggg ctggatttac gccgtgcgga gattgcccac
     8221 gagcgcgaag ggctgccctt aacgccgctg gcgaactgtg tggcagatac gcagggcggc
     8281 atcggctatc tgatccaaca ggcgctgaat aaccggctgg cgcgtcacgg cgagaagaaa
     8341 gccgtcaccg tggtgactca ggtggaagtg gataaaaacg atccaggttt tgcccatccc
     8401 accaagccca ttggtgcatt cttcagtgaa agccagtgtg acgaactaca aaaggcaaac
     8461 cctgactggt gttttgttga agatgccgga cggggctatc gccgcgtggt cgcctcgccg
     8521 gaaccgaaac gtattgtcga ggcacctgcc attaaggcgc tgatccaaca aggttttgtc
     8581 gtaattggcg cgggcggcgg tggaattccg gtagtgcgta ctgaagcggg ggattaccaa
     8641 agcgtggacg cggttatcga caaagatctc tctaccgcgc tgctggcccg tgaaattcac
     8701 gccgacattc tggtgatcac caccggcgtc gaaaaagtct gtatccactt tggcaaaccg
     8761 cagcagcagg cgctggatcg cgtagatatt gccactatga cccgctatat gcaggaaggg
     8821 catttcccac ccggcagcat gttgccaaaa atcatcgcca gcctgacgtt cctggaacag
     8881 ggcggcaaag aagtgattat caccacgccg gaatgcctgc ccgcggcgct gcgtggcgaa
     8941 acgggcaccc atattattaa aacgtaagga cgtaagatga aagaaaataa tagccgccgt
     9001 gaatttctga gccagagcgg taagatggtc accgccgccg cgctgtttgg tacctctgtg
     9061 ccgctcgccc atgcggcagt ctctggcaca acccactgcg acgcaaataa caccatgaaa
     9121 atcactgacc cgcattacta tctcgacaac gtgctgctgg aaactggatt tgactacgaa
     9181 aatggcgtgg cggtgcagac ccgcacggcg cgccagaccg tggagattca gaacggcaaa
     9241 attgttgcgc tgcgcgagaa taagcagcac ccggatgcca cgctgccgca ctatgacgct
     9301 ggcgataagc tgatgctgcc caccacccgc gacatgcata ttcatctcga caaaaccttt
     9361 tacggcgggc cgtggcgctc gctcaatcgt ccggcaggca ccaccatcca ggacatgatt
     9421 aagcttgagc agaaaatgct gccggagttg caaccgtaca ctcaggaacg ggcggagaaa
     9481 ctgatcgatt tattgcagtc gaaaggcacc accattgccc gcagccactg caatatcgaa
     9541 ccagtttccg gcctgaaaaa tctgcaaaat ttgcaggcgg tgctggcgcg acgtcaggcg
     9601 ggctttgagt gtgaaattgt cgccttcccg cagcacggtt tgctgctgtc gaaatcggaa
     9661 cctttaatgc gtgaagcaat gcaggcgggc gcgcattacg tcggcgggct ggacccgacc
     9721 agtgttgatg gcgcgatgga aaaatccctc gataccatgt tccagattgc gctggactac
     9781 gacaaaggcg tcgatattca cctgcacgaa accactccgg caggtgtggc agccatcaat
     9841 tatatggttg aaacggtaga gaaaacgcca cagctgaagg gcaagctgac catcagtcac
     9901 gcctttgcgc tggcaacgct caacgagcaa caggtagatg aactggcgaa ccggatggtg
     9961 gtgcaacaaa tttctatcgc ctcgacggtg ccgattggca cgctgcatat gccgctcaaa
    10021 cagttgcacg acaaaggcgt aaaagtgatg actggcactg acagcgttat cgaccactgg
    10081 tcgccttatg gtctgggcga catgctggaa aaagccaatc tgtacgcgca gctctatatt
    10141 cgtcctaacg aacagaacct ctcccgctcg ctgtttttag ccactggcga tgtattgccg
    10201 ctgaatgaaa aaggcgagcg tgtatggcca aaagcgcagg atgacgccag ctttgtgctg
    10261 gtggacgcct cctgttccgc cgaggcggtg gcgcgtatct cgccgagaac cgcaacgttc
    10321 cataaagggc aactggtgtg ggggagtgtg gcaggttgat gcgcatttga aacgcctgca
    10381 acggtgaata gtaagagatt taagccccag ggagtggggc aatctgaata tgagtgtgtc
    10441 ggcagaaatg gacattatct ttgaggaata tgcccttatt gacgttgtgt agataactat
    10501 ttgtctacgt gaaaagatca tcagttttgc cgattttagt cttttacaga tagcaaatat
    10561 cacacttaca ggcgctcgcc ttatggggaa cgttgctgct ctgtttcatt caggctcgcg
    10621 gcatgttggg gctggatcgg gtggtttaaa tattggctat attcaatgga cgcgttttgc
    10681 cgcgatgaca tatcaggcgt tgccaaatac acatagctaa tcaggagtaa acacaatgaa
    10741 gatcaaagct gttggtgcat attccgctaa acaaccactt gaaccgatgg atatcacccg
    10801 gcgtgaaccg ggaccgaatg atgtcaaaat cgaaatcgct tactgtggcg tttgccattc
    10861 cgatctccac caggtccgtt ccgagtgggc ggggacggtt tacccctgcg tgccgggtca
    10921 tgaaattgtg gggcgtgtgg tagccgttgg tgatcaggta gaaaaatatg cgccgggcga
    10981 tctggtcggt gtcggctgca ttgtcgacag ttgtaaacat tgcgaagagt gtgaagacgg
    11041 gttggaaaac tactgtgatc acatgaccgg cacctataac tcgccgacgc cggacgaacc
    11101 gggccatact ctgggcggct actcacaaca gatcgtcgtt catgagcgat atgttctgcg
    11161 tattcgtcac ccgcaagagc agctggcggc ggtggctcct ttgttgtgtg cagggatcac
    11221 cacgtattcg ccgctacgtc actggcaggc cgggccgggt aaaaaagtgg gcgtggtcgg
    11281 catcggcggt ctgggacata tggggattaa gctggcccac gcgatggggg cacatgtggt
    11341 ggcatttacc acttctgagg caaaacgcga agcggcaaaa gccctggggg ccgatgaagt
    11401 tgttaactca cgcaatgccg atgagatggc ggctcatctg aagagtttcg atttcatttt
    11461 gaatacagta gctgcgccac ataatctcga cgattttacc accttgctga agcgtgatgg
    11521 caccatgacg ctggttggtg cgcctgcgac accgcataaa tcgccggaag ttttcaacct
    11581 gatcatgaaa cgccgtgcga tagccggttc tatgattggc ggcattccag aaactcagga
    11641 gatgctcgat ttttgcgccg aacatggcat tgtggctgat atagagatga ttcgggccga
    11701 tcaaattaat gaagcctatg agcgaatgct gcgcggtgat gtgaaatatc gttttgttat
    11761 cgataatcgc acactaacag actgaaaaaa ttaataaata cgctgtggtt taacatatta
    11821 acttcgctct ccacttaact ttttagttaa ggagagcgaa ataatatcaa agtagcagta
    11881 aaacctataa cgtaaattca aattgttaaa ttaactccct ccagtacaca atatttcaca
    11941 cgttagttat gagcgatttc tgatagtgcc tggtttaatc agagctttat tatctgcgac
    12001 gtttattttt atttaagaga gatcgcgatg atatcattaa aagctccgca caataatttg
    12061 atgccatata cgcaacaaag catacttaat acggttaaaa acaatcagtt accagaggat
    12121 atcaaaagct ccctggtttc ctgtgtcgat atattcaagg ttttgattaa acaatattat
    12181 gattatcctt atgattgtcg tgatgattta gtcgatgacg acaaactcat tcatctcatg
    12241 gctgccgtac gagactgtga gtggtcagac gataacgcac tcaccataaa tgtgcagttt
    12301 aatgatttcc ctggatttta tgactggatg gattaccctg atcacccggt taagtttgtt
    12361 tttcgcatac ttgaaaatca aaaagggaca gtttgggtat atgaccagga tgatgcgttt
    12421 cttgatatta aagcaaacgt gcaggctgga cgctttaccg ggcttaagaa attagtgcag
    12481 tttatcgatt cagtgcgaac agattgcaaa tgtattcttc ttgaacacca tatgcctttg
    12541 ctgagaattt tccctaaagg gaaagagtgt atgcatgtgg aaaaatggct tcgcgaaatg
    12601 tcgtctattc cggagacaga cgcgcccatt aagcaggcgc ttgcgcatgg attattatta
    12661 cacttaaaaa atatctaccc tgtttttcct gaatcactgg tgatgttatt gctctctgtt
    12721 ctggacgtaa aaacatacag agatgatgcg cgattaaatc aatggatatc taatagagtc
    12781 caggaattag gcgacagata ttatcctgta aataagcacg ttaaaatccg ctacactctt
    12841 tagttcagaa agggttccac ctgcaagttg ttattccacc tgagagtgaa tcgcaatgca
    12901 ggtaatcatt tcatctgtat atctgtatgc caactggcat tccatctctt ttttcgccga
    12961 tattttgttg tgatcggcga cacttcgctt aaaaaagcac cagtagtggt ttcgcagcca
    13021 tgcggtgtat aaaaaatgat ctcatgcaga tgttttgtga atgtgttggt tgacattcat
    13081 atgaaaaaaa tcataattcc atcatgtttg tgtggggtct tttctgtatc ttacgcatcg
    13141 cactcaagcc tgacagaaaa tatgctgtaa ggctcatatc aaaaaccgcc attagctcat
    13201 caggaagagc agacgacaac caggattgtt gtatggtacg gggttcgagg cctcgatggc
    13261 ggtccaactt ttcaaaactc ttctgaatca gatcccctta ttatcatccc tgcaaagtgg
    13321 aacattgcca ttgtttggct attcaggatg gggcagacca atgaaaaaag cccacacagg
    13381 ggagagtggg ctgaaatggg aagctaaaga ctcaagtaaa cttatcggaa ataaggacca
    13441 cgcattacgg gggctatcat cgccgatggc cgtgattcgg caaatcaggt tgattacgta
    13501 gagagtaaat tatctgctca ccgctgcgtc accccttcgt aaatcaggcg tagcgcgaat
    13561 acaccaataa ttgcaccaat aacccgactg gcaacgcgtt gcatacgccc ataagcacga
    13621 cgcacagcgg gcaaagaaaa cgcctgacta agaaaaactc gccagataat tgatgcgagc
    13681 acaatccccg cccaggccat taaacgtgcc catgttggtg tttcggcatt taatgttact
    13741 gagaaaatac tgataaaaaa taaaacggtt tgcgggttag agagatcggt aattaatccg
    13801 cggcgaaaaa agacatacca gggggcgcta atcggttgtt gtagtgtgct catttgcggt
    13861 gttgactggc ggcgcatgct gcaccacgca aaccataaga gataagcgcc gccgacgatt
    13921 ctgataagcg aaaaaatctc ctcacactgc gtaattagcg ttgcaagacc aaacaaaccc
    13981 aacccggaat aaaatgcatc gcccagcgcc acgcccagcc cggtcagcac ccctgcgcgt
    14041 cgaccggaag ccaggctggt ttgtactacc acaaagagat tggctcccgg attaaaaaaa
    14101 gtaatcacga acagtcctac ggtcaggtaa acggcatgca aaggatccat agtgatttca
    14161 tccataaata agtgaactaa ctgcatcata tttctaccaa aaataatcgg gtgcgagaga
    14221 gatcacaaag tgtcttattt ccggttactg gcgtttatgc cctgactgaa ctaattatta
    14281 atcaacccaa taatgtgggt gggtgatagt gtgataacaa ctctggagcc gtaatatgaa
    14341 aataatctct aaaatgttag tcggtgcgtt agcgttagcc gttaccaatg tctatgccgc
    14401 tgaattgatg accaaagcgg aatttgaaaa agttgaatcg cagtatgaaa aaataggtga
    14461 tatttcaacc agcaatgaaa tgtcgactgc agatgcaaaa gaagatttga tcaaaaaagc
    14521 ggatgaaaaa ggggctgatg tgttggtact gacctccggt caaactgaca ataagatcca
    14581 cggcacggca aatatttata agaagaagta attctgaatc ctatgtaaac atctccgatg
    14641 cgtaagttta tcggtgatca tctattgaaa tttatgccgg ataaagcgtt cgcgctgcat
    14701 tcggcagttc agcttttcag ccgccgccag aacgtcgtcc ggctgatgcc taaataattc
    14761 gccgctgctg ttttatcgcc attaaatttc tccagtgcct gttgtggtgt cagtaagcgt
    14821 ggagcgggag ttttcgccga ctcgcgcgcc agttccggca gtagcagttg cataaactgc
    14881 ggcgttaaat ccggcgtcgg ttccacactt aaaaacagcg ccagtcgttc catcatattg
    14941 cgcagttcac gaatattgcc tggccagtcg tagtgcagca gcacagtttc acttgcctgt
    15001 aacccctggc gtaatgcagc agaaaatggg gcggagagcg ccgccagaga cactttcaaa
    15061 aagctttccg ccagcggaag aatatccgcc acccgctcgc gcagtggtgg caattgcaga
    15121 cgcaaaatac tcagccgata aaacagatca cggcgaaaac gtccttgctg catatcttct
    15181 tccagattgc agtgagtggc gctaatgacc cgtacatcta ccggaacagg ctgatgcccg
    15241 ccgacgcggg tgacctcttt ttcttccagc acccgcagca gccgggtctg caaaggtagc
    15301 ggcatttcgc caatctcatc cagaaacagc gtaccgccgt gggcaatttc gaacagcccg
    15361 gcgcgacctc cgcgtcgcga gccggtaaac gccccttcct catagccaaa cagttctgct
    15421 tccagcagcg attcggcaat cgccccgcag ttgacggcaa caaacggatg cgactttttg
    15481 ccctgtcgcg catcgtggcg ggcaaaatat tcccgatgaa tcgcctgggc cgccagctct
    15541 ttgcccgtcc ccgtttcccc ctcaatcaac accgccgcac tggagcgggc atacagcaaa
    15601 atagtctgcc gtacttgttc catctgtggt gattgaccga gcatatcgcc cagcacgtaa
    15661 cgagtacgca gggcgttgcg ggtggcatcg tgagtgttat ggcgtaacga catgcgcgtc
    15721 atatccagcg catcgctgaa cgcctggcgc acggtggcgg cggaatagat aaaaattccg
    15781 gtcattccgg cttcttctgc caaatcggta atcagccctg cgccgaccac cgcttcggtg
    15841 ccgttagctt ttagctcgtt aatctgcccg cgtgcgtctt cctcggtaat gtagctacgt
    15901 tggtcgaggc gcaaattaaa ggttttttga aacgccacca gcgctggaat ggtttcctga
    15961 taggtgacaa cgccgataga agaggtgagt tttccggctt ttgccagtgc ctgtaacaca
    16021 tcgtagccgc tcggtttaat caaaataact ggcactgaca ggcggctttt caggtacgcg
    16081 ccgttagagc cagccgcgat gatggcgtca cagcgttcgt ttgccagttt cttgcggatg
    16141 taggtcactg ctttttcaaa gccaagctga ataggggtaa tgttcgccag gtgatcaaac
    16201 tcgaggctga tatcgcgaaa cagctcgaac aggcgcgtta cagataccgt ccagataacc
    16261 ggtttgtcgt cattaagccg tggtggatgt gccatagcgc accgcaaagt taagaaaccg
    16321 aatattgggt ttagtcttgt ttcataattg ttgcaatgaa acgcggtgaa acattgcctg
    16381 aaacgttaac tgaaacgcat atttgcggat tagttcatga ctttatctct aacaaattga
    16441 aattaaacat ttaattttat taaggcaatt gtggcacacc ccttgctttg tctttatcaa
    16501 cgcaaataac aagttgataa caaaggatgg gctatgtctc tacactctcc aggtaaagcg
    16561 tttcgcgctg cactgactaa agaaaatcca ttgcagattg ttggcaccat caacgctaat
    16621 catgcgctgt tggcgcagcg tgccggatat caggcaattt atctttctgg cggtggcgtg
    16681 gcggcaggtt cgctggggct gcccgatctc ggtatttcta cccttgatga tgtgctgacc
    16741 gacattcgcc gtatcaccga cgtttgttcg ctgccgctgc tggtggatgc ggatatcggt
    16801 tttggttctt cggcctttaa cgtggcgcgc accgtgaaat cgatgattaa agccggtgcg
    16861 gcaggattgc atattgaaga tcaggttggt gcgaaacgct gcggtcatcg tccgaataaa
    16921 gcgatcgtct cgaaagaaga gatggtggat cggatccgcg cggcggtgga tgcgaaaacc
    16981 gatcctgatt ttgtgatcat ggcgcgcacc gatgctctgg cggtagaggg gctggatgcg
    17041 gcgatcgagc gtgcgcaggc ctatgttgaa gcgggtgccg agatgttgtt cccggaggcg
    17101 attaccgaac tcgccatgta ccgccagttt gccgatgcgg tgcaggtgcc gatcctcgcc
    17161 aacatcaccg aatttggtgc cacgccgctg tttaccaccg acgaattacg cagcgcccat
    17221 gtcgcaatgg cgctgtaccc actttcagcg ttccgcgcca tgaaccgcgc cgctgaacat
    17281 gtctacaacg tcctgcgcca ggaaggcacg cagaaaagcg tcatcgacac catgcagacc
    17341 cgcaacgagc tgtacgaaag catcaactac taccagtacg aagagaagct cgacaacctg
    17401 tttgcccgta gccaggtgaa ataaaaaacg cccgttgatt gtattcgaca gccgatgcct
    17461 gatgcgtcgc tgacgcgact tatcaggcct acgaggtgca ctgaactgta ggtcggataa
    17521 gacggatggc gtcgcatccg acaaccgatg cctgatgcgc cgctgacgtg acttatcagg
    17581 cctacggggt gcactgaact gtaggtcgga taagacgcat agcgtcgcat ccgacaaccg
    17641 atgcctgatg cgccgctgac gcgacttatc aggcctacgg ggtgcactga actgtaggtc
    17701 ggataagacg catagcgtcg catccgacaa ccgatgcctg atgcgccgct gacgcgactt
    17761 atcaggccta cggggtgaac tgaactgtag gtcggataag acgcatagcg tcgcatccga
    17821 caacaatctc gaccctacaa atgataacaa tgacgaggac aacatgagcg acacaacgat
    17881 cctgcaaaac agtacccatg tcattaaacc gaaaaaatct gtggcacttt ctggcgttcc
    17941 ggcgggcaat acggcgctct gcaccgtggg taaaagtggc aatgacctgc attaccgcgg
    18001 ctacgatatt cttgatctgg cgaaacattg cgaatttgaa gaagtggcgc atctgctgat
    18061 ccacggcaaa ctgccgaccc gtgacgaact cgccgcttac aaaacgaaac tgaaagccct
    18121 gcgcggttta ccggctaacg tgcgtaccgt gctggaagcc ttaccggcgg cgtcgcaccc
    18181 gatggatgtt atgcgcaccg gtgtttccgc gctcggctgc acgctgccag aaaaagaggg
    18241 gcataccgtc tctggcgcgc gggatattgc cgacaaactg ctggcgtcgc ttagctcgat
    18301 tctcctttat tggtatcact acagccacaa cggcgaacgc atccaaccgg aaaccgatga
    18361 cgactccatc ggcggtcact tcctgcatct gctgcacggc gaaaagccat cgcaaagctg
    18421 ggaaaaggcg atgcatatct cgctggtgct gtacgccgaa cacgagttta acgcctccac
    18481 ctttaccagt cgggtgattg cgggcaccgg ctctgatatg tattccgcga ttattggcgc
    18541 gattggcgca ctgcgcgggc caaaacacgg cggggcgaat gaagtgtcgc tggagatcca
    18601 gcaacgctac gaaacgccgg acgaagccga agcagatatc cgcaagcgcg tggaaaacaa
    18661 agaagtggtc attggttttg gtcatccggt ttacaccatc gctgacccgc gccaccaggt
    18721 gattaaacgt gtggcgaagc agctctcgca ggaaggcggc tcgctgaaga tgtacaacat
    18781 cgccgatcgc ctggaaacgg tgatgtggga gagcaaaaag atgttcccca atctcgactg
    18841 gttctctgct gtttcctaca acatgatggg cgttcccacc gagatgttca caccactgtt
    18901 tgttatcgcc cgcgtcaccg gctgggcggc gcacattatc gaacaacgtc aggacaacaa
    18961 aattatccgt ccttccgcca attatgttgg accggaagac cgcccgtttg tcgcgctgga
    19021 taagcgccag taaacctcta cgaataacaa taaggaaacg tacccaatgt cagctcaaat
    19081 caacaacatc cgcccggaat ttgatcgtga aatcgttgat atcgtcgatt acgtcatgaa
    19141 ctacgaaatc agctctaaag tggcctacga caccgcacat tactgcctgc tcgacacgct
    19201 cggctgcggt ctggaagctc tcgaataccc ggcctgtaaa aaactgctgg ggccaattgt
    19261 tcccggcacc gtcgtaccca acggcgtgcg cgtccccgga actcagttcc agctcgaccc
    19321 cgtccaggcg gcatttaaca tcggcgcgat gatccgctgg ctcgatttca acgatacctg
    19381 gctggcggcg gagtggggcc atccttccga caacctcggc ggcattctgg caacggcgga
    19441 ctggctttcg cgcaacgcgg tcgccagcgg caaagcgccg ttgaccatga aacaggtgct
    19501 gaccgcaatg atcaaagccc atgaaattca gggctgcatc gcgctggaaa actcctttaa
    19561 ccgcgtcggc ctcgaccacg ttctgttagt gaaagtggct tccaccgccg tggtcgccga
    19621 aatgctcggc ctgacccgcg aggaaattct caacgccgtt tcgctggcgt gggtggacgg
    19681 tcagtcgctg cgcacctatc gccatgcgcc gaacaccggc acgcgtaaat cctgggcggc
    19741 gggcgatgcc acttcccgcg cggtacgtct ggcactgatg gcgaaaacgg gcgaaatggg
    19801 ttacccgtca gccctgactg cgccggtgtg gggcttctac gacgtctcct ttaaaggtga
    19861 atcgttccgc ttccagcgcc cgtacggttc ctacgttatg gaaaatgtgc tgttcaaaat
    19921 ctccttcccg gcggagttcc actcccagac ggcagttgaa gcagcgatga cgctctatga
    19981 acagatgcag gcagcaggca aaacggcggc ggatatcgaa aaagtgacca ttcgcaccca
    20041 cgaagcctgt attcgcatca tcgacaaaaa agggccgctc aataacccgg cagaccgcga
    20101 tcactgcatt cagtacatgg tggcgatccc gctgctattc gggcgcttaa cggcggcaga
    20161 ttacgaggac aacgttgcgc aagataaacg cattgacgcc ctgcgcgaga agatcaattg
    20221 ctttgaagat ccggcattta ccgctgacta ccacgacccg gaaaaacgcg ccatcgccaa
    20281 tgccattacc cttgagttca ccgacggcac actatttgaa gaagtggtgg tggagtaccc
    20341 cattggtcat gctcgccgcc gtcaggatgg tattccgaaa ctggtcgata aattcaaaat
    20401 caatctcgcg cgccagttcc cgactcgcca gcagcagcgc attctggagg tttctctcga
    20461 cagagctcgc ctggaacaga tgccggtcaa tgagtatctc gacctgtacg tcatttaagt
    20521 aaacggcggt aaggcgtaag ttcaacagga gagcattatg tcttttagcg aattttatca
    20581 gcgttcgatt aacgaaccgg agcagttctg ggccgagcag gcccggcgta ttgactggca
    20641 gacgcccttt acgcaaacgc tcgatcacag caatccgccg tttgcccgtt ggttttgtga
    20701 aggccgaacc aacttgtgcc acaacgccat cgaccgctgg ctggagaaac agccagaggc
    20761 gctggcgctg attgccgtct cttcggaaac agaagaagag cgcaccttta cctttcgtca
    20821 gctgcatgac gaagtgaacg cggtggcctc aatgttgcgt tcattgggtg tgcagcgcgg
    20881 cgatcgggtg ctggtgtata tgccgatgat tgccgaagcg catattactc tgctggcctg
    20941 cgcgcgcatt ggcgctattc actcggtggt gtttggtgga tttgcctcgc acagcgtggc
    21001 ggcgcgaatt gatgacgcta aaccggtgct gattgtctcg gctgatgccg gagcgcgcgg
    21061 tggcaaaatc attccctata aaaaattgct cgacgatgcg ataagtcagg cgcagcacca
    21121 gccacgccat gttttgctgg tggatcgcgg gctggcgaaa atggcgcgcg tcagcgggcg
    21181 ggatgtcgat ttcgcgtcgt tgcgccatca acacatcggc gcgcgggtac cggtggcgtg
    21241 gctggaatcc aacgaaacct cctgcattct ctacacttcc ggcacgaccg gcaaacctaa
    21301 aggcgtgcag cgtgacgtcg gcggatatgc ggtggcgctg gcgacctcga tggacaccat
    21361 ttttggcggc aaagcgggca gcgtgttctt ttgcgcatcg gatatcggct gggtggtggg
    21421 gcattcgtat atcgtttacg cgccgctgct ggcggggatg gcgactatcg tttacgaagg
    21481 attgccgacc tggccggact gcggcgtgtg gtggaaaatc gtcgagaaat atcaggttag
    21541 ccggatgttc tcagcgccga ccgccattcg cgtgctgaaa aaattcccta ccgctgaaat
    21601 tcgcaaacac gatctctcgt cgctggaagt gctctatctg gctggagaac cgctggacga
    21661 gccgaccgcc agttgggtga gcaatacgct ggatgtgccg gtcatcgaca actactggca
    21721 gaccgaatcc ggctggccga ttatggcgat tgctcgcggt ctggacgaca ggccgacgcg
    21781 tctgggaagc cccggtgtgc cgatgtatgg ctataacgtg cagttgctta atgaagtcac
    21841 cggcgaaccg tgtggcgtca acgagaaagg gatgctggtg gtggaagggc cgctgccgcc
    21901 ggggtgtatt cagaccatct ggggcgacga cggccgcttt gtgaagactt actggtcgct
    21961 gttttcccgc ccggtgtacg ccacctttga ctggggcatc cgtgacgctg acggttatca
    22021 ctttattctc gggcgcactg acgatgtaat taacgttgcc gggcatcggc tggggacgcg
    22081 cgagattgaa gagagtatct ccagccatcc gggcgttgcc gaagtggcgg tggttggggt
    22141 gaaagatgcg ctgaaagggc aggtggcggt ggcgtttgtc attccgaaag agagcgacag
    22201 tctggaagat cgtgatgtgg cgcactcgca agagaaggcg attatggcgc tggtggacag
    22261 ccagattggc aactttggcc gcccggcgca cgtctggttt gtctcgcaat tgccaaaaac
    22321 gcgatccgga aaaatgctgc gccgcacgat ccaggcgatt tgcgaaggac gcgatcctgg
    22381 agatctgacg accattgatg atcctgcgtc gttggatcag atccgccagg cgatggaaga
    22441 gtaggttatt gtcggatgcg tcgcgcggtg catccggcac tgtgtgccga tgcctgatgc
    22501 gacgctgacg cgtcttatca tgcctacgga cctgaaccgt aggtcggata aggcgctcgc
    22561 gtcgcatccg acaccatgcg ccgatgcctg atgcgacgct gacgcgtctt atcatgccta
    22621 caggtctgaa ccgtaggtcg gataaggcgc tcgcgccgca tccgacacca tgctcagatg
    22681 cctgatgcga cgctgacgcg tcttatcagg cctacccact gtttttacac cgataatttt
    22741 tctcccacct ttttgcactc attcatataa aaaatatatt tccccacgaa aacgattgct
    22801 ttttatcttc agatgaatag aatgcggcgg attttttggg tttcaaacag caaaaagggg
    22861 gaatttcgtg tcgcaagata acaactttag ccaggggcca gtcccgcagt cggcgcggaa
    22921 aggggtattg gcattgacgt tcgtcatgct gggattaacc ttcttttccg ccagtatgtg
    22981 gaccggcggc actctcggaa ccggtcttag ctatcatgat ttcttcctcg cagttctcat
    23041 cggtaatctt ctcctcggta tttacacttc atttctcggt tacattggcg caaaaaccgg
    23101 cctgaccact catcttcttg ctcgcttctc gtttggtgtt aaaggctcat ggctgccttc
    23161 actgctactg ggcggaactc aggttggctg gtttggcgtc ggtgtggcga tgtttgccat
    23221 tccggtgggt aaggcaaccg ggctggatat taatttgctg attgccgttt ccggtttact
    23281 gatgaccgtc accgtctttt ttggcatttc ggcgctgacg gttctttcgg tgattgcggt
    23341 tccggctatc gcctgcctgg gcggttattc cgtgtggctg gctgttaacg gcatgggcgg
    23401 cctggacgca ttaaaagcgg tcgttcccgc acaaccgtta gatttcaatg tcgcgctggc
    23461 gctggttgtg gggtcattta tcagtgcggg tacgctcacc gctgactttg tccggtttgg
    23521 tcgcaatgcc aaactggcgg tgctggtggc gatggtggcc tttttcctcg gcaactcgtt
    23581 gatgtttatt ttcggtgcag cgggcgctgc ggcactgggc atggcggata tctctgatgt
    23641 gatgattgct cagggcctgc tgctgcctgc gattgtggtg ctggggctga atatctggac
    23701 caccaacgat aacgcactct atgcgtcggg tttaggtttc gccaacatta ccgggatgtc
    23761 gagcaaaacc ctttcggtaa tcaacggtat tatcggtacg gtctgcgcat tatggctgta
    23821 taacaatttt gtcggctggt tgaccttcct ttcggcagct attcctccag tgggtggcgt
    23881 gatcatcgcc gactatctga tgaaccgtcg ccgctatgag cactttgcga ccacgcgtat
    23941 gatgagtgtc aattgggtgg cgattctggc ggtcgccttg gggattgctg caggccactg
    24001 gttaccggga attgttccgg tcaacgcggt attaggtggc gcgctgagct atctgatcct
    24061 taacccgatt ttgaatcgta aaacgacagc agcaatgacg catgtggagg ctaacagtgt
    24121 cgaataacac tttacaaaca attattaacg cccggttacc aggcgaagag gggctgtggc
    24181 agattcatct gcaggacgga aaaatcagcg ccattgatgc gcaatccggc gtgatgccca
    24241 taactgaaaa cagcctggat gccgaacaag gtttagttat accgccgttt gtggagccac
    24301 atattcacct ggacaccacg caaaccgccg gacaaccgaa ctggaatcag tccggcacgc
    24361 tgtttgaagg cattgaacgc tgggccgagc gcaaagcgtt attaacccat gacgatgtga
    24421 aacaacgcgc atggcaaacg ctgaaatggc agattgccaa cggcattcag catgtgcgta
    24481 cccatgtcga tgtttcggat gcaacgctaa ctgcgctgaa agcaatgctg gaagtgaagc
    24541 aggaagtcgc gccgtggatt gatctgcaaa tcgtcgcctt ccctcaggaa gggattttgt
    24601 cgtatcccaa cggtgaagcg ttgctggaag aggcgttacg cttaggggca gatgtagtgg
    24661 gggcgattcc gcattttgaa tttacccgtg aatacggcgt ggagtcgctg cataaaacct
    24721 tcgccctggc gcaaaaatac gaccgtctca tcgacgttca ctgtgatgag atcgatgacg
    24781 agcagtcgcg ctttgtcgaa accgttgctg ccctggcgca ccatgaaggc atgggcgcgc
    24841 gagtcaccgc cagccacacc acggcaatgc actcctataa cggggcgtat acctcacgcc
    24901 tgttccgctt gctgaaaatg tccggtatta actttgtcgc caacccgctg gtcaatattc
    24961 atctgcaagg acgtttcgat acgtatccaa aacgtcgcgg catcacgcgc gttaaagaga
    25021 tgctggagtc cggcattaac gtctgctttg gtcacgatga tgtcttcgat ccgtggtatc
    25081 cgctgggaac ggcgaatatg ctgcaagtgc tgcatatggg gctgcatgtt tgccagttga
    25141 tgggctacgg gcagattaac gatggcctga atttaatcac ccaccacagc gcaaggacgt
    25201 tgaatttgca ggattacggc attgccgccg gaaacagcgc caacctgatt atcctgccgg
    25261 ctgaaaatgg gtttgatgcg ctgcgccgtc aggttccggt acgttattcg gtacgtggcg
    25321 gcaaggtgat tgccagcaca caaccggcac aaaccaccgt atatctggag cagccagaag
    25381 ccatcgatta caaacgttga acgactgggt tacagcgagc ttagtttatg ccggatgcgg
    25441 cgtgaacgcc ttatccggcc tacgtagagc actgaactcg taggcctgat aagcgtagcg
    25501 catcaggcaa ttccagccgc tgatctgtgt cagcggctac cgtgattcat tcccgccaac
    25561 aaccgcgcat tcctccaacg ccatgtgcaa aaatgccttc gcagcggctg tctgccagct
    25621 gtagtttatg ccggatgcgg cgtgaacgcc ttatccggcc tacgtagagc actgaactcg
    25681 taggcctgat aagcgtagcg catcaggcaa ttccagccgc agacctgtgt cagcggctac
    25741 cgtgattcat ttccgccaac aaccgcgcat ttatccaacg ccatgtgcaa aaatgccttc
    25801 gcggcggctg tctgccagct atttttccgc cgcaacaaaa ccgccgttct ctccagtagt
    25861 ggcggggcaa gagaaatagc tttaagcccg tcatgttgtg tggcaatcgc tgctggtaac
    25921 aatgtggaaa gggaagtgcg gcgaatcagc tccagaaccg cgctaattga gttcgcctca
    25981 atgaccacct gtggatgtag ccctgctttc tcgcagtagt ggtcaatttg ctctctggtg
    26041 gcaaattccg cgctgagcag gaccagtttt tcatcatgca agcgactcaa cgccacctgt
    26101 tcatggacgg ccagcggatg atgttgcgcc acgactaacg ctaaactttc tgtcagtaaa
    26161 ggaattgcct ccagctccgg cgaatgcaca ggcgcgaagg caatcccaac gtccaactcg
    26221 tcgcggcaaa gcatatcctc gattttctcc tgcgacattt cctgtagctg gagcgtgatg
    26281 ctgggatagc gcgcatagaa atccgccatt aaggggccga taaagtagct cgtaaaggtg
    26341 ggggtgacgg cgatacgcag cgatcctcgc gtcagatcgg caacatcatg aatcgcccgt
    26401 ttacccgccc ccagttcctg taacgcccgg ctggcgtact gtcgccagac ttctcctgca
    26461 tcagtgagac gaatcgttcg cccgctacgg tcaaacagcg gcacgcctaa actctcctct
    26521 aactggcgaa tctgctggga aagcgcaggc tgggagacgt gcaacgcact ggcggcacgg
    26581 gtgaagctgc catgttcagc cacggcaaga aaataattga tatgtcgaga gagcattcgc
    26641 aacctataag taaatccaat ggaactcatc ataaatgaga cttttacctt atgacaatcg
    26701 gcgagtagtc tgcctctcat tccagagaca gacagaggtt aacggtgaaa gagattattg
    26761 atggattcct taaattccag cgcgaggcat ttccgaagcg ggaagccttg tttaaacagc
    26821 tggcgacaca gcaaagcccg cgcacacttt ttatctcctg ctccgacagc cgtctggtcc
    26881 ctgagctggt gacgcaacgt gagcctggcg atctgttcgt tattcgcaac gcgggcaata
    26941 tcgtcccttc ctacgggccg gaacccggtg gcgtttctgc ttcggtggag tatgccgtcg
    27001 ctgcgcttcg ggtatctgac attgtgattt gtggtcattc caactgtggc gcgatgaccg
    27061 ccattgccag ctgtcagtgc atggaccata tgcctgccgt ctcccactgg ctgcgttatg
    27121 ccgattcagc ccgcgtcgtt aatgaggcgc gcccgcattc cgatttaccg tcaaaagctg
    27181 cggcgatggt acgtgaaaac gtcattgctc agttggctaa tttgcaaact catccatcgg
    27241 tgcgcctggc gctcgaagag gggcggatcg ccctgcacgg ctgggtctac gacattgaaa
    27301 gcggcagcat cgcagctttt gacggcgcaa cccgccagtt tgtgccactg gccgctaatc
    27361 ctcgcgtttg tgccataccg ctacgccaac cgaccgcagc gtaaccttat ttttaaacca
    27421 tcaggagttc caccatgatt cagtcacaaa ttaaccgcaa tattcgtctt gatcttgccg
    27481 atgccatttt gctcagcaaa gctaaaaaag atctctcatt tgccgagatt gccgacggca
    27541 ccggtctggc agaagccttt gtaaccgcgg ctttgctggg tcagcaggcg cttcctgccg
    27601 acgccgcccg cctggtcggg gcgaagctgg atctcgacga agactccatt ctactgttgc
    27661 agatgattcc actgcgtggc tgcattgatg accgtattcc aactgaccca acgatgtatc
    27721 gtttctatga aatgttgcag gtgtacggta caaccctgaa agcgttggtt catgagaaat
    27781 ttggcgatgg cattattagc gcgattaact tcaaactcga cgttaagaaa gtggcggacc
    27841 cggaaggtgg cgaacgtgcg gtcatcacct tagatggtaa atatctgccg accaaaccgt
    27901 tctgacagcc atgcgcaacc atcaaaagac gttcacgatg ctgctggtac tggtgctgat
    27961 tggtcttaat atgcgaccac tgctcacctc cgtcgggcca ctgctaccgc aattgcgcca
    28021 ggcgagcgga atgagcttta gcgtggctgc cctgttgacc gctctgccgg tggttaccat
    28081 gggcgggctg gcgctggccg gaagctggct tcatcagcat gtcagcgaac gtcgcagtgt
    28141 cgccatcagt ctgttgctga ttgccgtcgg tgcattgatg cgtgagtttt acccgcaaag
    28201 tgcgctgctg cttagcagcg cactgcttgg tggggtgggg atcggcatca ttcaggcggt
    28261 gatgccttcg gtgattaaac ggcggtttca gcagcgcacg ccactggtga tggggctgtg
    28321 gtccgcggct ctgatgggcg gcggtgggct tggtgccgcc ataacgccct ggttagttca
    28381 acatagcgaa acctggtatc aaacactcgc ctggtgggcg ctgcctgccg ttgttgcgct
    28441 ctttgcctgg tggtggcaaa gcgcccgcga ggtcgcctct tcccacaaga caacaaccac
    28501 tccggttcgc gtggtattca ctccccgcgc gtggacgctg ggtgtttact tcggtctgat
    28561 taacggcggt tacgccagcc tgattgcctg gttacccgct ttctatattg agattggtgc
    28621 cagcgcgcag tacagcggtt ccttactggc attgatgacg cttgggcaag ccgcaggagc
    28681 tttgctgatg cctgctatgg ctcgccatca ggatcggcgc aaactgttaa tgctggcgct
    28741 ggtgttacaa ctggtggggt tctgcggctt tatctggctg ccgatgcaat tgccggtatt
    28801 gtgggcgatg gtgtgtgggt taggtctggg cggcgcgttt ccgctctgtt tgctgctggc
    28861 gctcgatcac tctgtgcaac cggctattgc tggcaagctg gtggcgttta tgcagggaat
    28921 cggttttatc atcgccgggc ttgccccgtg gttttctggc gtgctgcgta gtatcagcgg
    28981 caattacctg atggactggg catttcatgc gctgtgcgtc gttgggctga tgatcataac
    29041 cctgcgtttt gcaccagtac gttttccgca gctgtgggtc aaagaggcat gatgcgacgc
    29101 ttgttcctgc gctttgttca tgccggatgc ggctaatgta gatcgctgaa cttgtaggcc
    29161 tgataagcgc agcgtatcag gcaattttta taatttaaac tgacgattca actttataat
    29221 ctttgaaata atagtgctta tcccggtcgt ttatttcgcg aataacccga caaggaacgc
    29281 cagccgccac gacgtttggt ggaatgtctt ttgtgacgat actacccgcg ccaataacag
    29341 aattatcccc gatggtgacg cctggattaa taaccacatg acttccgatc cagacgttat
    29401 tgccaatcgt tatcggaaaa gagtacatct cgccgttttt tctcaattca tggtgtacag
    29461 ggtgtcccgt aacggaaaga gtaacgttgg gtgcaatcag tacattatca ccgattgtta
    29521 ccgtgtagtc atcgacaatg gttaaattga aatttgcata aaaattgcgg cctatatgga
    29581 tgttggaacc gtaagagaaa tagacaggcg gttctaccca ggcgttttcc cctaccgtgg
    29641 caaacatttc tttaatcagg ctttctcttt tttcaacttc tgatggatgc gagtgattaa
    29701 actcatacat taacgttttc ccacgaagtc ttttttccgg taagccttcg cacatatcgg
    29761 taaatagctt gcctgctctt attctttcgg tcattggcat gttcaatgcg atcactccgt
    29821 tatgatatgt tggtcggata aggcgctcgc gccgcatccg acattgattg cttaagcgac
    29881 ttcattcacc tgacgacgca gcagggaaag cgggccgggg ccgctaagcg tgaacacgga
    29941 aattaaggtg aagcccagcg ccaccagacc cagcaccaga taagcgccct ggaaaccgat
    30001 gctttcatac atattgcccg ccagtacaga cataaaaatc atcgccagtt gcttaaagaa
    30061 gcagaaacag accagataaa tcgtcgctga aaaacgcact tcaaactggc tggtaatata
    30121 tttaaagcag cccaccagca ggaacggtac ttcaaacata tgcagcgttt tcagaataac
    30181 cacttccagc gctgaggtgg cgaacgatga gccaataata cgtacagaca taatagtgcc
    30241 agccagcagc agggcgtttt tcccaccgat gcgattaatg atcagtggcg caaagaacat
    30301 aatcgaggcg ttaagtaatt cgcccattgt cgttacgtag ccaaataccc gcgtaccctg
    30361 ttcaccggta gcaaagaacg aagtaaagaa attagcaaac tgttggtcaa aaacatcgta
    30421 ggtgcaggaa acgccaataa catacagtga caaaaaccac agttttggct gtctgaacag
    30481 ttccagtgcc agcttaaggc taaatgccga atggttggca cctaccgcat tggcaaccgt
    30541 ggcagaagag ggcgcatccg ttttggcgaa aaagagtaaa acggcgagga tgagtgcaca
    30601 gccagagccc agccagaaaa caaactgatt attgatggtg aacatgatgc cgacaatcga
    30661 ggcacacagc gcccagccaa cacagccaaa catccgcgcg cgaccaaatt cgaaattact
    30721 gcgacggctg actttctc
//
LOCUS       DAPHBJ010000032        30627 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.032, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000032 DAPHBJ010000000
VERSION     DAPHBJ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.032"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1316
                     /locus_tag="RHZ76_001363"
     CDS             <1..1316
                     /locus_tag="RHZ76_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HDV2812025.1"
                     /translation="IFDNVMLYMMFFYTDIFGIPAGFVGTMFLVARALDAISDPCMGL
                     LADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPDLSMNGKMIYAAITYTLLTLLYTV
                     VNIPYCALGGVITNDPTQRISLQSWRFVLATAGGMLSTVLMMPLVNLIGGDNKPLGFQ
                     GGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMREDLRDIWQNDQWRIVGLLTIFNI
                     LAVCVRGRAMMYYVTWILGTPEVFVAFLTTYCVGNLIGSALAKPLTDWKCKVTIFWWT
                     NALLAVISLAMFFVPMQASITMFVFIFVIGVLHQLVTPIQWVMMSDTVDYGEWCNGKR
                     LTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDAAEKAQNSATISIIIALFTIVPAI
                     CYLLSAIIAKRYYSLTTHNLKTVMGQLAQGKRRCQQQFTSQEVQN"
     gene            1326..3644
                     /gene="yicI"
                     /locus_tag="RHZ76_001364"
     CDS             1326..3644
                     /gene="yicI"
                     /locus_tag="RHZ76_001364"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HDV2812026.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIIGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTGLVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVFKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKFPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKETVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANAQGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTESG
                     DVQFYLPEGRWTHLLHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYAW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVLFTLKAARSGNTITVTGAGEARNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(3697..5406)
                     /locus_tag="RHZ76_001365"
     CDS             complement(3697..5406)
                     /locus_tag="RHZ76_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDV2812027.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRLADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVINARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANIPLKPTVSGQLHAVNAEKQQVTQTMNAGIVSSGEVTSTE
                     PVQ"
     gene            complement(5527..6918)
                     /gene="xanP"
                     /locus_tag="RHZ76_001366"
     CDS             complement(5527..6918)
                     /gene="xanP"
                     /locus_tag="RHZ76_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HDV2812028.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            7197..8402
                     /gene="gltS"
                     /locus_tag="RHZ76_001367"
     CDS             7197..8402
                     /gene="gltS"
                     /locus_tag="RHZ76_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDV2812029.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANLASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLADTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(8436..10517)
                     /gene="recG"
                     /locus_tag="RHZ76_001368"
     CDS             complement(8436..10517)
                     /gene="recG"
                     /locus_tag="RHZ76_001368"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDV2812030.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLATCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVGLMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDSRRNDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQLLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(10523..11212)
                     /gene="trmH"
                     /locus_tag="RHZ76_001369"
     CDS             complement(10523..11212)
                     /gene="trmH"
                     /locus_tag="RHZ76_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HDV2812031.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADRDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(11219..13327)
                     /gene="spoT"
                     /locus_tag="RHZ76_001370"
     CDS             complement(11219..13327)
                     /gene="spoT"
                     /locus_tag="RHZ76_001370"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HDV2812032.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(13346..13621)
                     /gene="rpoZ"
                     /locus_tag="RHZ76_001371"
     CDS             complement(13346..13621)
                     /gene="rpoZ"
                     /locus_tag="RHZ76_001371"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDV2812033.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(13676..14299)
                     /gene="gmk"
                     /locus_tag="RHZ76_001372"
     CDS             complement(13676..14299)
                     /gene="gmk"
                     /locus_tag="RHZ76_001372"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDV2812034.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            14557..16242
                     /gene="ligB"
                     /locus_tag="RHZ76_001373"
     CDS             14557..16242
                     /gene="ligB"
                     /locus_tag="RHZ76_001373"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HDV2812035.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGEETHRDAMMPPLNGAVMHPVAHTGVR
                     KMADKNALNLWMRERNDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVR
                     LISAVPRTVSGPLANSTLQGEIFLKRKGHIQQQMGGINARAKVAGLMMRQDDSDTLNS
                     LGVFVWAWPDGPQLMTDRLKELATAGFTLTQTYTRAVKNADEVARVRNEWWKAKLPFV
                     TDGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASL
                     APVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPT
                     PPENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFKHIF
                     SWLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDER
                     SWSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(16239..16856)
                     /locus_tag="RHZ76_001374"
     CDS             complement(16239..16856)
                     /locus_tag="RHZ76_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HDV2812036.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(17148..17972)
                     /gene="dinD"
                     /locus_tag="RHZ76_001375"
     CDS             complement(17148..17972)
                     /gene="dinD"
                     /locus_tag="RHZ76_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HDV2812037.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(18194..19057)
                     /locus_tag="RHZ76_001376"
     CDS             complement(18194..19057)
                     /locus_tag="RHZ76_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDV2812038.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIIARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            19184..19900
                     /gene="rph"
                     /locus_tag="RHZ76_001377"
     CDS             19184..19900
                     /gene="rph"
                     /locus_tag="RHZ76_001377"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDV2812039.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            19966..20607
                     /gene="pyrE"
                     /locus_tag="RHZ76_001378"
     CDS             19966..20607
                     /gene="pyrE"
                     /locus_tag="RHZ76_001378"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDV2812040.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(20644..21240)
                     /gene="slmA"
                     /locus_tag="RHZ76_001379"
     CDS             complement(20644..21240)
                     /gene="slmA"
                     /locus_tag="RHZ76_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HDV2812041.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYATDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(21347..21805)
                     /gene="dut"
                     /locus_tag="RHZ76_001380"
     CDS             complement(21347..21805)
                     /gene="dut"
                     /locus_tag="RHZ76_001380"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDV2812042.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(21783..23003)
                     /gene="coaBC"
                     /locus_tag="RHZ76_001381"
     CDS             complement(21783..23003)
                     /gene="coaBC"
                     /locus_tag="RHZ76_001381"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDV2812043.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVASEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            23175..23843
                     /gene="radC"
                     /locus_tag="RHZ76_001382"
     CDS             23175..23843
                     /gene="radC"
                     /locus_tag="RHZ76_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDV2812044.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            24060..24296
                     /gene="rpmB"
                     /locus_tag="RHZ76_001383"
     CDS             24060..24296
                     /gene="rpmB"
                     /locus_tag="RHZ76_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDV2812045.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            24317..24484
                     /gene="rpmG"
                     /locus_tag="RHZ76_001384"
     CDS             24317..24484
                     /gene="rpmG"
                     /locus_tag="RHZ76_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDV2812046.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            24582..25391
                     /gene="mutM"
                     /locus_tag="RHZ76_001385"
     CDS             24582..25391
                     /gene="mutM"
                     /locus_tag="RHZ76_001385"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDV2812047.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEEFPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIMATKHAQRATFYCRQCQK"
     gene            complement(25430..25909)
                     /gene="coaD"
                     /locus_tag="RHZ76_001386"
     CDS             complement(25430..25909)
                     /gene="coaD"
                     /locus_tag="RHZ76_001386"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDV2812048.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(25917..27194)
                     /gene="waaA"
                     /locus_tag="RHZ76_001387"
     CDS             complement(25917..27194)
                     /gene="waaA"
                     /locus_tag="RHZ76_001387"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HDV2812049.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            27607..28665
                     /gene="rfaQ"
                     /locus_tag="RHZ76_001388"
     CDS             27607..28665
                     /gene="rfaQ"
                     /locus_tag="RHZ76_001388"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HDV2812050.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKSSEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRNSYTHLVPLQGGNVVESNLSVLSPLGLESLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            28662..29786
                     /locus_tag="RHZ76_001389"
     CDS             28662..29786
                     /locus_tag="RHZ76_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDV2812051.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYAQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDRVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            29779..30576
                     /gene="rfaP"
                     /locus_tag="RHZ76_001390"
     CDS             29779..30576
                     /gene="rfaP"
                     /locus_tag="RHZ76_001390"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HDV2812052.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVAMMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         7178 a         7669 c         8229 g         7551 t
ORIGIN      
        1 ttattttcga taacgtaatg ttatatatga tgttctttta taccgatatt tttggcatac
       61 ctgccggatt tgtcggaacc atgtttttgg tcgctcgtgc actggatgcg atttccgatc
      121 cttgcatggg gttgttggcc gatcgaacgc gctctcgctg gggtaaattt cgtccatggg
      181 tactgtttgg cgcactgcca tttgggatcg tctgtgtact ggcctatagc acgccagatc
      241 tcagcatgaa cggaaaaatg atttatgcag caattactta caccctactt accttacttt
      301 ataccgtcgt taatatccct tactgcgcat tgggtggtgt aatcaccaat gacccgactc
      361 agcgtatctc gctgcaatcc tggcgttttg tgctggcaac cgcgggaggc atgctctcta
      421 ctgtgctgat gatgccgctg gttaatttaa ttggtggtga taataaacca ctcggtttcc
      481 agggcggtat cgcggtcctt tccgtggtgg cattcatgat gctggcattt tgtttcttca
      541 ccactaaaga acgcgttgaa gcaccaccta caacgacgtc tatgcgggaa gatttacgtg
      601 acatatggca aaacgaccag tggcggattg tcggtttact aaccattttc aatatcctgg
      661 cggtgtgcgt acgcggtagg gcgatgatgt attacgtcac atggattttg ggtacgccgg
      721 aagtgtttgt cgcttttctc accacttatt gcgtgggtaa cctgattggt tccgcactgg
      781 caaaaccgct gaccgactgg aaatgtaaag tcactatctt ctggtggacg aacgccctgc
      841 tggcagtgat tagcctcgcg atgttctttg ttcccatgca ggccagtatc actatgtttg
      901 tcttcatctt cgtgattggt gtgttgcatc aactggtgac acctatccag tgggtaatga
      961 tgtccgatac cgttgactac ggtgagtggt gcaatggtaa acgcctgacc gggatcagtt
     1021 ttgctggcac gttgtttgtg ctcaaactgg ggttggcctt cggcggcgct cttatcggct
     1081 ggatgctggc ttatggcgga tatgatgcgg cagaaaaagc gcagaacagc gccacgatta
     1141 gcatcattat cgcgctattc acgattgttc cggcgatctg ttacttgctg agcgcgatta
     1201 tcgctaaacg ctactactca ctcacgacac acaatctgaa aaccgttatg ggacagctgg
     1261 cccagggcaa acgccgttgc cagcaacaat tcacctctca agaagtgcag aactaaggaa
     1321 cggcaatgaa aattagcgac ggaaactggt tgattcaacc tggcctcaat ttgattcacc
     1381 cgcttcaggt gttcgaggtt gaacagcagg gtaatgaaat ggtggtctat gctgcccccc
     1441 gtgatgtgcg tgaacgtacc tggcagcttg acacgccttt atttacgctg cgctttttct
     1501 ccccacagga aggtattatc ggtgtacgga ttgagcattt tcagggggcg ctgaataacg
     1561 gtcctcatta cccgctcaat attttgcagg acgtgaaggt cacaatcgaa aacacagaac
     1621 gttacgctga gtttaaaagt ggcaacttaa gcgcgcgtgt cagcaaaggt gagttctggt
     1681 cactggattt tctgcgcaac ggcgaacgta ttaccggtag tcaggtgaaa aataatggct
     1741 acgtgcagga cacgaataat cagcgaaatt atatgtttga gcggctggat cttggcgttg
     1801 gcgaaacagt ttacggtctg ggagagcgct ttactggcct ggtgcgcaat ggccagacgg
     1861 tagagacctg gaaccgggac ggcggcacaa gtactgaaca ggcgtataaa aatattccgt
     1921 tctatatgac taaccgtggt tatggtgtat tggtcaatca tccccaatgc gtctcttttg
     1981 aagtgggatc ggagaaagtc ttcaaagtgc agttcagcgt tgagagtgaa tatctcgaat
     2041 actttgttat cgacggcccg acgccgaaag cggtacttga tcgttatacc cgttttactg
     2101 gtcgtccggc gctgccgccc gcgtggtcct tcggcctgtg gctaaccact tcatttacca
     2161 ccaactacga cgaagcgacg gtaaacagct ttatcgatgg tatggcggaa cgcaatctgc
     2221 cgctgcatgt tttccacttt gactgtttct ggatgaaagc cttccagtgg tgcgattttg
     2281 agtgggaccc gctgactttc ccggacccgg aagggatgat ccgccgcctg aaagcgaaag
     2341 ggctaaaaat ctgcgtctgg attaacccct atatcgggca aaaattccct gtctttaaag
     2401 agttacaaga gaaaggctat ttactcaaac gcccggacgg ttcgttatgg cagtgggata
     2461 aatggcagcc aggtctggcg atttatgact ttaccaatcc ggatgcctgc aaatggtacg
     2521 ccgacaaact gaaaggtctg gtcgcgatgg gcgttgattg ctttaagacc gactttggcg
     2581 aacgtattcc aaccgatgtt caatggtttg acggttccga tccgcagaaa atgcataacc
     2641 attatgcgta catctacaac gaactggtgt ggaatgtgct caaggaaacc gtcggcgagg
     2701 aagaagccgt cttgtttgct cgctcggcct ccgtcggtgc gcagaaattc ccggtacact
     2761 ggggtggcga ctgttacgct aactacgaat caatggcgga aagcctgcgc ggtggtttgt
     2821 ctattggcct ttcaggtttt ggcttctgga gccacgatat cggcggcttt gagaataccg
     2881 ctccggcgca cgtttacaaa cgctggtgcg cgtttggttt gctctccagc catagccgtt
     2941 tacacggcag caaatcttat cgtgtgccgt gggcctatga tgatgagtcc tgtgatgtgg
     3001 tgcgcttctt cacgcaactg aaatgccgca tgatgccgta tctgtatcgt gaagcagcgc
     3061 gtgcgaacgc gcagggtacg ccgatgatgc gggccatgat gatggagttc ccggacgatc
     3121 cggcttgtga ttaccttgac cgtcaataca tgttaggcga caacgtgatg gttgctccgg
     3181 tgttcactga atcgggcgat gtgcagttct acttgccgga aggtcgctgg acacacctgt
     3241 tgcacaacga tgaactcgac ggtagtcgct ggcataaaca gcagcacggc ttcctgagtc
     3301 tgcccgtata tgtgcgtgat aacaccctac tggcgctggg caacaacgat caacgtcccg
     3361 attacgcgtg gcacgaaggc acggcattcc acctctttaa tctgcaagac gggcatgaag
     3421 ccgtctgtga agtgcccgct gctgacggtt ccgttctttt caccctgaaa gcggcgcgtt
     3481 ctggcaacac aattactgtg actggtgcgg gcgaggcgag gaactggacg ctgtgcttgc
     3541 gcaatgttgt gaaagtaaat ggtctgcaag gcggttcgca ggctgaaagt gagcaggggc
     3601 tggtggtgac gcctcaaggg aatgcgctga caattacgtt gtaatcaatt gaagaaatgc
     3661 cgggtacgtc tcaatcgtac ccggcgagat tgatttttac tgcacaggct ccgtcgatgt
     3721 aacttcgccg ctggaaacga tgccagcatt catggtctgg gtcacttgct gtttttcagc
     3781 atttacggcg tgaagttgcc cgctaaccgt aggtttcagc gggatattgg cctgaatatc
     3841 gccgctggcg gtaagctgaa tattgccgtc gccagtcaac ggtaattcag gccatcccca
     3901 ctgttgcaaa atattcaccg gcacaccgcg tccattcagg ctgatatggg tctgacgttg
     3961 tggtgtttgt gaaacactgg cggtggcttc cagaatgcct ttttcagtaa atgcactcag
     4021 ttcgcttata ttcaccgtgc tgctgttggc ggtcagcgcc agcgacgggc gacgaacatc
     4081 aacacgattg aatgtcgcgg cggcggcatt cagattcgcc gaaccactcc agacgcccca
     4141 tttatgatcg gtaaccagcg tcaggttggc accgtaacca tcgagcgcgg tgagctgcca
     4201 cgggaagtca gggtcgatat caatgatcag attgcggctg gcgctaaatc tcttcagttg
     4261 caggctgttt aaccaacccg gtgtcgtttc catccacaac tgttgccagt ttttcggcaa
     4321 ggtatattcc agcccggcaa ttgccgcatc atcaaggatc aacgttttcc cgtcacgcag
     4381 ccagttccct gacgttctga ccataccccc ttcccagcgg ctggtgaact ggcgcagcgc
     4441 tacgccctgc ggggaaaatt ccgcgtttat aatcgggtca aataaatgca gcgaaccata
     4501 aatgaactcg ctggcgttca tcgacagttt gccttcttgt gtctgccagt catctttact
     4561 gaaggtcatg ttgcgcaagc tgagatcgag gtcggtcacc gcccagtccg gaccttgcag
     4621 acgagcgttg atcacttcca ggcgaccaat ctgcaacgac gggacagagc gtaatggcgc
     4681 aaagaagtcg gttagcgatt tttcgctttg tagacggata tccgccagcc gcagattttc
     4741 cacttgccag ctgccgtcgg cgttacgctg cgcgtttccg gttaatgtcc cgcgggcgat
     4801 gtcggcaccc aggttagtca gcgtaacgcg atcgttatca atactgcctt caatcagtac
     4861 attggtggca ggaacatcat tgagcgaaag cgatccggca ctaaactgaa tctgcgcctt
     4921 cgtacccagc actttaccgg cttctggtga ccacggaacc acgccaccat ttacccgctg
     4981 cgcgctcagt ttccattcgc tattcgggct attaaacgcc atatcacgca gttgcagacg
     5041 atcggctttg aacggtagcg gcgcggtctg gtcggtgaga ttcagcgtcc cgttttccag
     5101 caggatggta tcgacatggc gtggttcggt cagttgccga ctgcttagcg caatgtcgac
     5161 actttttgcc accagggtcg cgggctgacc atcacgacca aacgtgacgt tctccagcac
     5221 gatatgagat ggcgcggaaa aacggtgatc catcgccccg aaggccagat gatagtcgct
     5281 attctcggaa acccatgcgc tgatatgttc tgctccccag cgggtttgca gaagaaaata
     5341 gaggccagcg atcaccacta acagagcgat gagaatgtag agaagcagct tcccaataaa
     5401 tttcatggtc ttccatcccg tgaaatgcac ataagggagt tatgcacgat ttacgcgcaa
     5461 tcctcaaggc gggaatggtg aaagaggtga gactgcggcg gtaattatct taccgccgcc
     5521 agtgaattac tgtttttctg gtgggaaaat cagattcagc acgatggcag taataccgcc
     5581 cgcggcgatc ccggaggaga gcaggttttt cagccactca ggggcaaact gcaaaatcag
     5641 cggctgctga gagacgccca gaccaaccgc cagcgacagc gcgataatca gaatcgcccg
     5701 acggttcagc ggctcacgag acacaatgcg cacaccggag gcggcgatgg tgccaaacat
     5761 tacaagcgtt gcgccgccca gaaccggttc tggaatatgt tgtacaaaac cgctcactgc
     5821 cgggaacaga cccagcacga tcaacatcag cgcgacgaca aaaccgacat agcggctggc
     5881 aaccccggtc aactggatca ctccgttgtt ctgcccgaag caggagttcg ggaaggtgtt
     5941 aaacaccgcc gaaacaaacg agttcaggcc gtttgccagc acgccgcctt tcaggcgttt
     6001 catgtacagc ggaccggaca ctggctgttc ggaaacgtca gaggtcgccg tgatatcgcc
     6061 aatggtttcc agtgaagtga tcataaagac cagcatcagg ggtagcagca gactccattc
     6121 aatgccaaga ccgtaataga gcggcgttgg caccataatc agttcttgcg tcatcggttc
     6181 gttgctttct ggcaacatgc ccataaacca cgccagcgca tatccggccg ccatcgcaat
     6241 caccagtgag gccacgcgca aatatgggtt acgctgacgg ttaagcagga taattaaggc
     6301 taagaccacg cccgccagca gcagattttt cggtgcgccg aaggtgttat cgctcatggc
     6361 tgcgtaaccg ccgccaatgg aggtcagccc cacctgaatt agcgacaggc cgataatcat
     6421 caccacaacg ccagaaacca gcggcgtaat aatgcggcgc gccagatgca gaacgcggga
     6481 gatcaccatc tcggtacaac ttgccagcat caacgtgccg aataaagccg ccatcatggt
     6541 aggaacatca gcaccaccgg tcttcagcgc tgtgccgccc ataatcagcg gagcaacaaa
     6601 gttgaagctg gtgccctgaa tagacaacaa cccggagcca accggacccc aggccttaat
     6661 ttgaataatc gatgccacac cggaggcaaa cagcgacata ctaataatgt gttgcgtgtc
     6721 ttgtgccggt aaacccagcg cctggcagat taatagcgct ggcgtgatca ccgcaacgaa
     6781 catcgccagc aaatgctgac aggcggcaaa cagggtttga ggaagcggcg gacgatcttc
     6841 aagacggtaa atcagttcgc tgttttgagt ctgcgcaacc ggttgcgcat tttctgactc
     6901 gagggtggaa acagacatcg aaaacaatcc cgtggtagca aagcgggcat tttagctgac
     6961 cgaaagagaa aagcaaacgt ttgccacatc ctcatctaaa tatttaaatg agataaatgc
     7021 acttttttat ataacttttg gtatttttct gcttaaaatc catgccattt gttcattaac
     7081 agctgagaat ttgacccagg aaagcataac tcgatacccg tcatagttca taagttgcca
     7141 ctgcgcgatg aagtatgacg agtatgaaag agtgatgcgg atacaaagga gtaactatgt
     7201 ttcatctcga tactttagca acgcttgttg ccgcaacgct gacgttgctg ctcgggcgta
     7261 agttggtcca ttccgtctcc tttttgaaga aatacaccat accggaacca gttgcgggtg
     7321 gtttgttggt ggcgctggcg ctactggtac tgaaaaaaag catgggctgg gaagtcaact
     7381 ttgatatgtc cctgcgcgat ccgttaatgc tggctttctt cgccaccatt ggtttgaacg
     7441 ccaaccttgc cagtctgcgt gccggtgggc gcgtggtagg tatcttcctg atcgtcgttg
     7501 ttggtctgtt ggtgatgcaa aatgccattg gcattggtat ggctagcctg ttagggcttg
     7561 atccgctgat ggggctgttg gccggttcta ttacgctttc cggcggtcac ggtacgggag
     7621 ctgcatggag taaattgttc attgaacgtt atggcttcac caatgcgacg gaagtggcga
     7681 tggcctgtgc aacgttcggt ttggtgctgg gcggcttgat tggcggtccg gtggcgcgct
     7741 atctggtgaa acactccacc acgccgaacg gtattccgga tgaccaggaa gtcccgacgg
     7801 cgttcgaaaa gccggatgtg ggccgcatga tcacctcgct ggtgctgatt gaaactatcg
     7861 cgctgattgc tatctgcctg acggtgggga aaattgttgc gcaacttttg gctgacactg
     7921 cttttgaact gccgaccttc gtctgtgtac tgtttgttgg cgtgattctg agcaacggtc
     7981 tgtcaatgat gggcttttac cgcgtctttg agcgtgcggt atccgtgctg ggtaacgtaa
     8041 gcctgtcgtt gttcctggcg atggcgctga tggggctgaa actgtgggag ctggcttcgc
     8101 tggcgctgcc gatgctggcg attctggtgg ttcagaccat cttcatggcg ttgtatgcca
     8161 tcttcgttac ctggcgcatg atgggcaaaa actacgatgc ggcggtgctg gctgcgggtc
     8221 attgtggttt tggcctcggt gcaacgccaa cggcaatcgc caacatgcag gcgatcactg
     8281 aacgctttgg cccgtcgcac atggcgtttt tggtggtgcc gatggtcggt gcgttcttta
     8341 tcgatatcgt caatgcgctg gtgattaagc tgtatttgat gttgccgatt tttgcgggct
     8401 aaccgatgaa gcggcggtct tctcaccgcc gccttttacg cattcgagta acgttccgtc
     8461 tccggcatcc agcgttctat cagggctttt gcttgttgtg ggtaacgttc gtgaatatgt
     8521 cgcgccagga gctgaacttc cgggatcatc gcctgatcgc gcagtaaatc cgccacttta
     8581 aactcagcat tacccgtctg acgcgtgcct aacaattcgc cagggccgcg aatctccaga
     8641 tctttttgcg caatcacaaa cccgtcgttg ctgtcgcgca gtacttgcag acgaatttgc
     8701 gccgttttag agagcggtgt tttgtagagc agcacgcagt gagaagccac cgcaccacgt
     8761 cccacgcgcc cgcgcaactg gtgtaactgc gccaggccca ggcgctccgg gttttcgata
     8821 atcatcaggc tggcgttagg cacatcaacg ccgacttcaa taacggtagt ggcaaccagc
     8881 aggtgtaact cgccttgttt aaatgatgcc atgacggcct gcttctcggc tggtttcatc
     8941 cgtccgtgta ccaggccgac attcagttct ggcaacgcca gtttcaattc ttcccatgta
     9001 gcttccgccg cctgcgcttc gagtaattcc gactcttcaa tcaacgtaca aacccagtac
     9061 gcctgacgcc cttcagtcat acaggcgtgg cgcacgcggt cgataatgtc gttgcggcgg
     9121 gaatccggga ttgccactgt cgtcactggc gtgcggcctg gcggcagctc atctatcacc
     9181 gaggtatcga gatcggcata tgcagtcatt gccagcgtgc gggggatcgg cgtggcggtc
     9241 atgatcaact gatgcggatg gaagccctgc tgctggcctt tctcccataa cgccagacgc
     9301 tgatgcacgc caaaacgatg ctgttcgtcg ataatcacca gcgccaggcc gttaaactgc
     9361 acctgttcct ggaagatggc gtgggtgcca acaatcatct gcacctgacc gctggcgatg
     9421 gcttcttgct gagccagtcg tgctttacct ttctgtttac cggcgagcca gcccacttcg
     9481 ataccgagcg gagcaaacca gttacggaag ttattggcgt gctgctcggc gagtaattcg
     9541 gttggtgcca tcaatcccac ctgtttgccg tgcgcaatcg cacgcaacgc ggcgagggcg
     9601 gcgaccagcg ttttaccgga acctacatcg ccctgcacca ggcgcatcat cggcacatcc
     9661 agcgccatat cgcgctcgat ctccgccacc acgcgtgcct gtgcgcccgt tggcttgaac
     9721 ggtaaggcgg cgaggagttt atttttcagc gcgtcattgg cgctcagcgg ctgggcatga
     9781 aaacgctgtg ctccggcacg taaggctaac atgctgaggt tgtgcgccag caactcttcc
     9841 agaatcagtc ggcgttgtgc tggatgctgc ccggtttcga gatcgctaag ctgtagcgtc
     9901 ggtggcgggc gatgcaaagt gcgcaaggct tccggcagcg tcatcattcc ttgtgacagt
     9961 tccggcggca ggagttcttc aatggcgcag gtggcgagca gatccagcgc ctgatcggtt
    10021 aatttacgca gcgtggcctg ctttacgcct tccgttgttg ggtaaaccgg cgttagcgtt
    10081 tcctgcaact ctggcgtact gagatcgccc tgcacgcggt attccgggtg gatcatctct
    10141 gcaccatatt taccgcgctt tgcttcgcca taggccagca cgcggcggcc tgtcgccagg
    10201 ctatttttca ttgccgcatt gaagttgaaa aagcgcatgg tgagaatgcc ggaaccgtcg
    10261 ctgatctggc aggtcatcat ccgccgaccg ccgaaggaga tattgcaatt cagtacttcg
    10321 ccttctaccg tggcataaac gcccggcagt agctcaccga tggggtagag atgggtgcga
    10381 tcttcgtagc gcaggggaag gtgtaagagt aaatcctgca cggtatgcag gttgattttc
    10441 gccagcttgt tactaagcgc tgcgccaacg cccgttaggg aactgagcgg gacagcatct
    10501 aacaggcgac ctttcatggc acttaccctg cagcctgcat agtagcccac cagtcggcat
    10561 cagcttcgat ctcgccttgc tgattgacgt gggggtaagg caggcctttg cgttttgcga
    10621 ctttcgccag caccggatag ccgccctcaa acagcaggcg ttgttgctct gcttccggca
    10681 acatgctgtt ttcacgcagg tacatgcctg cattttgccg ctggcgctgg gcttcgtaaa
    10741 gaatgagggc tgaggcaacg gaaacattca gcgactgcac cataccgatc atcggtataa
    10801 tgatgtcccg atccgccagg gctaacgctt cctgagtgat gcccgttttc tcctgaccca
    10861 tcaaaataca ggtcgggcgg gtgtaatcaa tttcgcggaa atcgacagcg ttatcagaaa
    10921 gatgtgttgc caggatctgc atgccctggc ctttcagatg agcgacggca tcgccaatgg
    10981 tgcggtgcgt tttcacctgc acccaactgt tgctgccagc cgcagccgaa gccattgtgc
    11041 gcatccgact gccaggccag acagcatgaa cttcatgtac gccaacggca tctgcagtac
    11101 gaataatcgc agaaacgtta tgaggtttgt ggacctgctc catgcagacg gtcagatcag
    11161 gctgccgcct ggcgagcatt tcgcagatgc gtgcataacg tgttgggttc ataaaacatt
    11221 aatttcggtt tcgggtgact ttaatcacgt ctggcatcac gcggattttg cgcatgatat
    11281 ttgccagatg tacgcggtca cgggcggtca gacgaataaa ggcgctgtag acacgaccat
    11341 ctttctcttc cgtattcaaa ctttgaatat tcgaggtcgt ggtgttaatt gccgccgtca
    11401 ggtttgccag cgcgccctga tgattgaaca tctccacttt gatttcggtg atgaactcct
    11461 gcgccgtctc tttatcccat tccacagcca taaacttctc tggctctttc tggtagccac
    11521 ggatgttacg gcaggattca tggtggatca ccagaccttt accggggctg acgtgggcga
    11581 taatcgggtc gccaggaata gggcggcagc atttcgcaaa ggtgatcagc acgccatcgg
    11641 cacctttaat tggcagatgt ccgtgacttt gagttgccgg tggaatggag gcgtccccat
    11701 gttgcagatt tttcgcgacc accacgctca ttgcgttacc aaggccgatt tcagccagca
    11761 gatcgtcaag cgtagccagc ttcatgcgat ccagctcgcg ctgaatattt tcctgcggga
    11821 tttcattgag cttacggcta ccacccaacg catggttgag cagacgacgt cccaggctta
    11881 cagaatcatc acgcttgagg tttttcagca actgacgaat tttggcgcgc gctttcgagc
    11941 tgacgacaaa gttcagccac gcggcattcg ggcgagcgcc aggtgcggta atgatttcaa
    12001 cggtttgacc gctggtcagc ggctgcgaca gcgggtaagg ctggcggtcg acgcgcgcgc
    12061 ccacgcaggc atgaccgata tcggtatgca ccgcataagc gaagtcgacg ggcgttgcac
    12121 cggcaggcag ctctacaatg cgcccttccg gtgtgaaaac gtaaatctca tccgggaaga
    12181 gatcggattt aacgctctcg ataaattcaa acgaactacc ggcgctctgt tgcagctcca
    12241 gcaggctttg catccagcgc tgggcgcgga tttgcgcggt agtactggtt tcgccgtgct
    12301 ctttataagc ccagtgcgcg gcaacaccca tctctgccat ctgatccata tcttcggtac
    12361 ggatctggac ctcaaccggc acgccgtgcg ggccaatcat cgaggtgtgc aaagactgat
    12421 agccgttcgc ttttggaatg gcgatgtagt ctttcacgcg acccggacgc ggcttgtaca
    12481 ggctatgcat ttgcccgagc acgcgataac aggtgtcgga atcattgacg atcacgcgga
    12541 aagcgtagat gtccatgatt gagtgaaaac gctgctcttt gagcaccatt ttgcagtaga
    12601 tcgaataaag atgcttctcg cgaccactga cgcggcacgg tattcccgct tcctgcaaac
    12661 gcccttcgat ttccgagagg attttttgga tcatctcttt acggttgccg cgcgcggctt
    12721 tcaccacttc tttgattacg cgataacggt tgggatacag cgcctcaaaa cccagctctt
    12781 cgagttcggt tttaatgtgg tggataccta aacggtgtgc cagcgggctg taaatttcga
    12841 gggtttcacg ggcgatgcgg cgacgtttgt ccgggcgaag tgagcccagc gtgcgcatgt
    12901 tgtgggtacg gtcggcaagt ttgatgagaa tgacgcggat atcctgcacc atcgccataa
    12961 tcatcttgcg aaagttttcg gcctgcgcct ctttcttatc gcggaacttg agcttatcaa
    13021 gtttcgacac cccctctacc agctcggcga cgcttttacc aaaaagctgt tccatgtcct
    13081 ggtaggtggc gggagtatct tcaatcacgt catgcagcag cgccgccatt agcgtttcat
    13141 agtcgagttt catctcggcc agaatgcagg caaccgctac cgggtgcgtg atatagggtt
    13201 caccgcttga acgtgtttgc ccctcgtgag catcacgtgc aacgagatac gcctgccgca
    13261 gacgcttgat ttggtcttcc ggcaggtagg tttgaatcag ttgattcagg ctttcaaaca
    13321 gatacaaggg cgacccgctt tgtgattaac gacgaccttc agcaatagcg gtaacggctt
    13381 gtaattcagc ggcttcctgc tcttgctgtt cctggcgttc acgaacgtcg aggatctggt
    13441 tgttgatcag accttcttcg atttcgcgca gcgcgattac agtggtttta tcgttttctt
    13501 ctggtaccag cggatccttt ccgcctacct gcatctgacg agcgcgacgc gcggcgacca
    13561 gtaccaggtc aaaacggtta ccaattttct ctacagcgtc ctgaacagtt acgcgtgcca
    13621 tacttaaaaa gctccacagg tgaagaaatg actgggcatg atactgaaat caggttcagt
    13681 ctgccaacaa tttgctgatt aaagcgtcat gacgctgctt ttggcggctc atgcgcagac
    13741 gttcggcgcg aataatggtc ttcaaatcgg tcaacgcggt atcgaagtca tcattcacaa
    13801 tcaggtaatc atattcggcg taatggctca tttctgcaac agcttgcgcc atacgctttg
    13861 caatgacctc ttcgctgtcc tgaccgcgac cgcgtagacg gcggtccagt tcaattttgg
    13921 acggcggtaa aataaagata ctccgcgcgt gcggcatctt ctggcgaatt tgctgcgcgc
    13981 cctgccagtc gatatcgaga aaaacatcga caccggtcgc cagtacttgc tcaatggcct
    14041 cacgcgaagt gccatagtaa ttaccaaaaa cttctgcgtg ttcgaggaac gcatctctgc
    14101 taatcatttc tttaaattca tcgtgattaa caaagaaata atgttcaccg tggacttcac
    14161 caggacgcgg ttggcgtgtg gtgtgtgaaa cagaaacctg ggtgtcatac aacggttggg
    14221 tttttaataa agcctgaatc aggctggatt tacccgcgcc actgggggca gaaacaatat
    14281 aaagcgtgcc ttgagccatg agtatctttc gtatgtgatt agcgaaataa agcctacata
    14341 cgagcttatt atacacggcg cggccatgtg acgtagccct tgctcacact ttttgcgcaa
    14401 agttgttgcg ttttgcgtag ctgcttccgc atttgcctgt gattcactgc aataaagacg
    14461 attctcagga agcgtcctcg cagactccgt ttttgccact cggctttgct ttcatcacgt
    14521 tgtatatctg cctgactatc tacagcggag gtgatgatga aagtatggat ggcgatatta
    14581 ataagtatct tgtgctggca atcatctgtg tgggcggtct gtccggcctg gtcgccagcc
    14641 agagcacagg aagaaatttc ccgcctgcaa cagcaaataa aacagtggga cgatgactac
    14701 tggaaagaag ggaaaagtga ggtggaagac ggggtttacg atcagttaag cgcccgtctc
    14761 acgcagtggc aacgctgttt tggtgaagag acgcatcgcg acgctatgat gccgccttta
    14821 aacggtgcgg ttatgcatcc ggttgcacat acgggtgtac gcaaaatggc ggataaaaac
    14881 gcattaaatc tatggatgcg cgaacgcaac gatctttggg tgcagccaaa agttgatggc
    14941 gttgcggtaa ccctggttta tcgggacggg aaactgaaca aagcaatcag tcgcggcaat
    15001 ggcctgaaag gcgaggactg gacgcagaaa gttcgcttaa tttccgctgt gccgcgaacc
    15061 gttagcgggc ctttagctaa tagtacgctt cagggggaaa tatttctcaa gcgtaagggg
    15121 catatccaac aacaaatggg gggaataaat gcccgcgcaa aagttgctgg cctgatgatg
    15181 cgccaggacg atagcgacac gctgaattca ctgggcgttt ttgtctgggc atggccggat
    15241 ggaccgcagt taatgaccga tcgtttaaag gagctggcta ccgcaggttt tactctaacg
    15301 cagacgtata cccgtgcggt gaaaaatgct gatgaggttg cgcgcgtacg caatgagtgg
    15361 tggaaagcga aattaccctt cgtcaccgat ggcgtggttg tgcgagcggc taaggaaccc
    15421 gaatcccgtc attggctacc gggccaggcg gagtggctgg tggcctggaa atatcaacct
    15481 gtagctcagg ttgccgaagt gaaggcgatt cagtttgcgg tgggtaagag cggtaaaata
    15541 tcggtggttg cgtcactcgc acctgtcatg ctggatgata aaaaagtcca gcgggtgaat
    15601 attggttccg tcaggcgctg gcaggaatgg gatattgcgc caggtgatca gatcctcgtc
    15661 agcctggccg gtcagggtat tcctcgcatt gatgatgtgg tctggcgcgg tgcagaacgt
    15721 acaaaaccga caccaccaga aaaccgcttt aactcgttga cctgctactt tgcttctgat
    15781 gtttgtcagg aacagttcat ttcacgctta gtctggctgg ggtcaaaaca ggttcttggg
    15841 ctggatggca ttggtgaggc cggctggcgc gcgctgcatc agactcatcg ctttaagcat
    15901 atcttttcct ggcttttatt aacgccagag caattacaga acacgccggg gatcgcgaaa
    15961 agtaaaagtg cgcagttatg gcatcagttt aatctggctc gtaagcagcc ttttactcgt
    16021 tgggtgatgg caatgggaat accgctaacc cgggcggcgc ttaatgccag tgatgaacgg
    16081 tcctggtcgc aacttttatt tagcacggaa cagttctggc agcagctgcc ggggactgga
    16141 tcggggcgag ccagacaggt tattgaatgg aaggaaaatg cgcaaatcaa gaagctgggc
    16201 agttggctgg ctgcccagca aatcacaggt tttgaacctt agtgaccttc gtggctgtag
    16261 taaaagaccg gtaatcccag cttaagacgt agcgccagta aacgggcaaa aaagccgaat
    16321 accagggtgg agataatcac cacatcatgg ttagaaacat agtgttgcag cgcgatgtac
    16381 agcacggcgg aggcaaatga gacaccggca tacaactctt tctggaatac cagtgggatg
    16441 cgtttacaga acatatcgcg caaaacgccg ccaaatacgc cggtcgtcac agctgcgaca
    16501 acggcaatga tcgggccgtg tcccatatcc agtgcaacct gtgcgccaat gatagaaaag
    16561 actaccagtc cgagcgcatc gagcaccaga aacactttgc gcaggtaagg cattacagga
    16621 gcaacaatag tggtgagtac tgcggcggta gcgacgataa tcacatattc cgggtgtttg
    16681 acccagccga gcggatagtg gcccagcaga atatcgcgca ctgaccctcc accaattgcc
    16741 gtggcggtcg caataataat tacgccaaat gtatccatgc gccgtcgtcc ggccgccagc
    16801 gcccccgtca tggcttcggc agtaatgcca accaaataca aaatgtgtaa cagcatggtt
    16861 ctctccctaa aaatctgtct ggcagggtag cgatttgtgc gcattgtcac gattgagatt
    16921 ttttaaggtg agaggaaatt gataacttgt agttatgtga aatcgttgtt ttctttagcg
    16981 tagatgcatt cttctgactt cttgtctgag aaaatgcata agaaaaagta ggttaaaatt
    17041 gaggggtgga aaagaaaggt gcaatgttta gccattgtgt tcaccgcacc tggactttgg
    17101 cttacgcatc gggtatctca atcaacaaaa agagttttta gtttctgcta tttcttctct
    17161 gtaattttta cactgttttc caactgcttg atgctgacct gcggggtcgg caactcctca
    17221 ggcatggttc cgccaagttc ctgaatggtt tgcctcacct tgctacccac gtcaaagtgt
    17281 gtggtattag cttgctgttt tgaattcacg ccatcccgct tgagtttttc ttctgtttgg
    17341 gtagctcgaa agagattagc cgccagttct gttgaaccca tatgatccag gatcttctga
    17401 ctctttttca gccccttccg ctgatggatg gctttctgat ctaatccacc atacagtccc
    17461 cggtaaccat gattctgaaa tatggcgaaa tcgatagctg tcgctacccc agcttgctgc
    17521 gctgcctcaa ctaattgttt gttatgttct ttcaactcat ttctcagaaa aagacgcttt
    17581 tcgtcttcac gaagttgtct gaaagcttca tcgtcagcca gctcctgccg tcgggtctgg
    17641 atagcaaaat aagtttgccc tgccgcaata accggtttcg cagggtcgcc gttttgtact
    17701 accaggtaac aggcatagcg ggagagatga acatcttcaa gttctcgttg agcccctgaa
    17761 cctaatacaa ccattttggt ggtttcaacg aaatggtcag atgcagcttg attgctggct
    17821 tcgcacgctt gagtcgcgcg ggccagtacc ttttgaaaat tgcgccagtc ccgataatcc
    17881 agtaacggtg ccaattctct cgctgaccaa aattcttgcc cttcagtgcc ataatgccgt
    17941 atttcttcaa aaggttgatg atgttcgttc attgcccact ccatgtgata tcgattgcaa
    18001 ggaaagtaag tctacaactg tgtttatata cagttgtgag tatctcacgt tctcactgcg
    18061 ctgggcaaaa aaacatcgca aaatctggag caggcacgca aaacagaatt gaatgttgcg
    18121 gggaggaaaa atgccgctct gtttgagtgg gcggcatttg tgaaggctga aaaggaagaa
    18181 ttggcgttac gagttattca atgttctgga tctgctcgcg catctgctca atcaacactt
    18241 tcagctcgat ggcagaattc gtcacttcgg cattgataga tttcgacgca agagtgttcg
    18301 actcgcggtt gaactcctgc atcataaagt ccagacgacg accaaccgct tcttttttct
    18361 tcagaatgtt gtaggtctct ttgacatgcg cttcgaggcg atccagttct tcggcaacat
    18421 caattcgttg tgccagcaga accaactcct gctccaggcg gttgttttcc agttgcactt
    18481 gagcatcttc cagcttcgcg accagacgct cacgctgcca ttgcaggatt tccggcatat
    18541 gggcgcggac tttgactact tcggcggtga cgccttccag acgctgctcg atcaatgctt
    18601 tcagcgcctg accttcggtt tcacgtgcga tgataaagtc gtccagcgta ccatccagcg
    18661 ccgcgagaat ttcagcggca atcgcgtcga gatcctgctc ctgggctgcc attacgcccg
    18721 gccagcggag aatatcaacc gggttgattt ccccttcgtc actctgcatt ttcacccagt
    18781 tcgcggcagt taccagctgt ttagccagtt tttcgttgag gatcagctca ccttgtgcgc
    18841 taacatctgg ctcatagcgc agggtacatt ccactttacc gcgcgtcagg cgagaacgaa
    18901 tgcgctcacg aacgacaggt tcaaggctac ggaactgctc cggcagacga aagtaagttt
    18961 ccagataacg ctggtttacc gagcgcattt cccaggttgc gctcccccat tcacccttga
    19021 tttcacgccg ggcgtaggcg gtcatactgc ggatcataga cgttcctgtt tataaaagga
    19081 gaggtggaag gattatagcc atcgatgcct tgtcaggata ggaataaccg ccggaagtcc
    19141 gtataatgcg cagccacatt tgtttcaagc cggagatttc aatatgcgtc cagcaggccg
    19201 tagcaataat caggtgcgtc ccgttaccct gactcgtaac tatacaaaac atgcagaagg
    19261 ctcggtgctg gtcgaatttg gcgataccaa agtgttgtgt accgcctcta ttgaagaagg
    19321 cgtgccgcgc ttcctgaaag gtcagggcca gggctggatc accgcagagt acggcatgct
    19381 gccacgttct actcacaccc gtaacgcgcg tgaagcagcg aaaggtaaac agggtggacg
    19441 cacaatggaa atccagcgtc tgatcgcccg tgctcttcgc gcggcagtag atttgaaagc
    19501 gctgggtgag ttcaccatta cgctggactg cgacgtgctc caggctgatg gcggtacgcg
    19561 taccgcttcg atcaccggtg cctgcgtggc gctggcagat gcgctacaga agctggtgga
    19621 aaacggcaag ctgaaaacca acccgatgaa agggatggta gccgcagttt ctgtcggaat
    19681 tgtgaacggc gaagcggttt gcgatctgga atacgttgaa gactcagccg cagagaccga
    19741 catgaacgta gtgatgaccg aagacgggcg catcattgaa gtgcagggga cggcagaagg
    19801 cgagccgttc acccatgaag aactgctcac cttgttggcg ctggcccgag ggggaatcga
    19861 atccattgta gcgacgcaga aggcggcgct ggcaaactga tttttaaggc gactgatgag
    19921 tcgccttttt tttgtctgta gaaaagtaag atgaggagcg aaggcatgaa accatatcag
    19981 cgccagttta ttgaatttgc gcttagcaag caggtgttaa agtttggcga gtttacgctg
    20041 aaatccgggc gcaaaagccc ctatttcttc aacgccgggc tgtttaatac cgggcgcgat
    20101 ctggcactgt taggccgttt ttacgctgaa gcattggtgg attccggcat tgagttcgat
    20161 ctgctgtttg gccctgctta taaagggatc ccgattgcga ccactaccgc cgtggcgctg
    20221 gcggagcatc atgaccttga cctgccgtac tgctttaacc gcaaagaggc aaaagaccac
    20281 ggtgaaggcg gtaatctggt tggtagcgcg ttacaaggac gcgtaatgct ggtagatgat
    20341 gtgatcaccg ccggaacggc gatacgcgag tcgatggaaa ttattcaggc caatggcgcg
    20401 acgcttgctg gcgtattgat ttcgctcgat cgtcaggaaa gggggcgcgg cgagatttcg
    20461 gcaattcagg aagttgagcg tgattacaac tgcaaagtga tctctatcat caccctgaaa
    20521 gacctgattg cttacctgga agagaagcca gaaatggcgg aacatctggc ggcggttaag
    20581 gcctatcgcg aagagttcgg cgtttaaaga aactcgccgg atgaaaagtc atccggcgtc
    20641 atattactgc aactgtgcgg caattagcgg ccagcgggcg tcaaaatcat ccgttgggcg
    20701 gtatttaaac tcgctgcgga caaaacgtga cagcatacct tcacagaagg ccaggatctg
    20761 gcttgccagc agggtttcat ccgtcgcgta gccttcaccc tcacgcattc tcttttcacg
    20821 caatacctga cgcaactgcg cttcaatacg ctcgaacagc tggttgatgc gcccttgcag
    20881 gcgatcctgt tcaaacatta gtgcatgacc agtgaggatg cgggtcaggc caggattacg
    20941 ctcaccaaaa ccgagaagca gcaacacaat cagacgcagg cgcgctgtgg tgtccttctc
    21001 atctttcaga atcagattga tgcgagtaat caggctatct tcgataaact caatcaggct
    21061 atcgaacatg cgggtcttac tggggaagtg gcgatacagt gccgcttcgg aaacgccgac
    21121 agaggcggcc agttttgccg tcgtgatacg ttggcttcca tcgctggatt ccagcatcag
    21181 cgccagagac tgaagtattt cctcgcgacg gttccttttc gcagtttgtt tttctgccat
    21241 gttacaaaat acccctgaaa ataagcactt gccaggcggc acccacgcta tgaccgcaaa
    21301 cgaaatgttt gcggctatgt tatgacgtta ttcggatgcg tatgtgttac tgacgaccag
    21361 agtgaccaaa gccgccttca ccgcggtcgg tggcgtcgaa atcttccacc agattaaatt
    21421 cagcctgtac taccggaaca aaaatcatct gggcgatgcg ctcgccaggt tgaatggtga
    21481 agctgtcctg accacggttc cacacggaaa tcatcaactg gccctgatag tcagaatcga
    21541 tcaatcctac caggttacca agcacgatac cgtgcttatg tcccaatccg gagcgcggca
    21601 gcatcattgc cgccagtgaa ggatcggcaa tatgaatcgc cagcccggtc ggaaccagcg
    21661 tagtgtcacc cggagccagt tctacggcgt cgtcgagaca ggcacgcagg tcaagtccgg
    21721 cagagccaga ggtggcataa gtcgggagcg gaaattcctt cccaacgcgc gggtccagaa
    21781 tcttaacgtc gatttttttc atcataacgg gtcacgatct cgtcgagtaa taattggcca
    21841 aggagctctt tgcgctcaag cggtaagact ttatctccgt cctgccagaa aaggtgtaat
    21901 gcgttgttgt cgctgttaaa tccttgagtt ggctgggaaa catcgttcgc gcagatcaga
    21961 tcaaggtttt tacggatacg tttttgccgg gcgtattctt ccacattatt tgtttcggcg
    22021 gcaaatccaa cgacgtaggg tcgatggtct tttagtgcgg caacgcctgc gacgatatcg
    22081 gggtttttaa ccatttttat tgttaattca tcaccctgcg tggcctgctt tttgattttt
    22141 tctgaggcca cggtagctgc gcgataatcc gccacggcgg cgcaaccgat aaaaatattt
    22201 tgctgctgta cagaagcatt cacggcggct tccatttcca gcgcggtcat cacatcaaca
    22261 cgtttaacaa acggtggcgt cggtagcgaa accgggcctg ataccagcgt gacgttcgcg
    22321 ccacgacggg cagcagcggc ggcgatagca aaacccatct tgcctgagct gtgattagag
    22381 atataacgca ccggatcgag cggttcacgc gtcgggccgg cggtaatcat aatgttcaga
    22441 tgtttcaggt cgttgacggg cgaaaaatgc gctactgcca tatcaacaat ggttaacgga
    22501 tcgagcattc gccctgggcc gatatcacca caagcctgac tgccactgtc tggcccccag
    22561 atgagcaaac cacgggaggc aagcacctct aaattatgct gcgtggcagc ggcacggtac
    22621 atctgctggt tcatggcggg gagcacggct acaggcgcag gagtggccag acaaatcgtc
    22681 gataccagat cattcgccat tccggcagca acacgggcaa tcaaatctgc cgtggcaggg
    22741 gcgagaatca ctaaatcagc ccatttaccc agctcaatat ggcccatagc ggcttcggct
    22801 gccgggtcca gcagactgtc ggaaacggga taaccagaaa ccgcctgcaa gctaagtggg
    22861 gtgataaagg cttttgccgc ttcggtcatg gctacgcgga cgtcggcccc gcgatcgcgc
    22921 aaacgacgca ccagttcagg ggttttatag gcagcaatac cgccgctgac gccgagaacg
    22981 atttttttac cggccaggct catgatgatt tttcctgttg ggtgacacca gaagttggcg
    23041 attttatcac aatacttttg ttgtcgtgcc ttcacctgag aatcactttg cgaggcgctt
    23101 tccaggattg aaaactggcc gtcgatttaa cggaacggct atgacaggat gcgagcacca
    23161 caaaggaggt gaaggtgaaa aacaatgcac agctgttgat gccgcgcgaa aaaatgctga
    23221 agtttggtat tagcgcctta acggatgtcg agctgctggc gctatttctg cgtaccggaa
    23281 cgcgcggtaa agatgtatta accctggcaa aagagatgct ggagaatttc ggctctcttt
    23341 atggcttgtt aacctctgaa tatgaacagt ttagtggcgt tcatggaatt ggtgtggcga
    23401 aattcgccca gttaaagggg attgccgaac tggcgcggcg ttactacaac gtgcggatgc
    23461 gtgaagaaag ccctttactc agcccggaga tgacgaggga atttttacaa agccagctca
    23521 cgggtgagga gcgggagatc tttatggtga tctttctcga ctcccaacac agggttataa
    23581 cgcatagccg tcttttttcc ggcacgctaa accatgttga agtccatcct cgggaaatta
    23641 tccgcgaagc gataaaaata aacgcctcgg cgctgatcct tgcacataat cacccttcgg
    23701 gttgtgctga acccagtaaa gcggataaac tcatcaccga acggataata aagagttgtc
    23761 agttcatgga tttacgcgtg ctcgaccata tcgtgattgg gcgtggagag tatgtttctt
    23821 ttgccgaacg cggctggatt taacccgcta tgcgcgatcc ttcgggatct ttgtctgttc
    23881 gggacttgag cacatcgctg agtcagcgta tactacgcca cctttgagaa tctcgggttt
    23941 ggcatttggg cctggcaatc gagagttcac atagaactgc gatgaccggg ctgtaaagcc
    24001 tgacgaggcg ccaatacccc atacgaagct cgagctaatt tgatttttgg agaatagaca
    24061 tgtcccgagt ctgccaagtt actggcaagc gtccggtgac cggtaacaac cgttcccacg
    24121 cactgaacgc gactaaacgc cgtttcctgc cgaacctgca ctctcaccgt ttctgggttg
    24181 agagcgagaa gcgttttgtc accctgcgcg tatctgctaa aggtatgcgt gtaatcgata
    24241 aaaaaggcat cgatacagtt ctggctgaac tgcgtgcccg tggcgaaaag tactaagtac
    24301 ttagaggaaa taaatcatgg ctaaaggtat tcgtgagaaa atcaagctgg tttcttctgc
    24361 tggtactggt cacttctata ccactacgaa gaacaaacgt actaagccgg aaaaactgga
    24421 actgaaaaaa ttcgatccag ttgttcgcca gcacgtgatc tacaaagaag cgaaaatcaa
    24481 ataatttttg ctttgatgta acgaaaaacc tcgccccggc ggggtttttt gttatctgcc
    24541 tgcccccata ttgactgcat ctgttcattc ctggagatgc tatgcctgaa ttacccgaag
    24601 ttgaaaccag ccgtcgaggc atagaaccgc atctcgttgg tgcaaccatt cttcatgcag
    24661 tggtgcgcaa cggacgcttg cgctggccgg tttcagaaga gatttaccga ttaagcgacc
    24721 aaccagtgct tagcgtgcag cggcgggcta aatatctgct gctggagctg cctgagggct
    24781 ggattatcat tcatttgggg atgtctggca gcctgcgcat ccttccagaa gaatttcccc
    24841 ctgaaaagca tgaccatgtg gatttggtga tgagcaacgg taaagtgctg cgctacaccg
    24901 atccgcgccg ctttggtgcc tggctgtgga ccaaagagct ggaagggcat aatgtgctgg
    24961 cccatcttgg accggagccg cttagcgacg atttcaacgg tgagtatctg catcagaagt
    25021 gtgcgaagaa aaaaacggcg attaaaccgt ggctgatgga taacaagctg gtggtagggg
    25081 tagggaatat ctatgccagc gaatcgctgt ttgcggcggg gatccatccg gatcggctgg
    25141 cgtcatcact gtcgctggca gagtgtgaat tgttagctcg ggtgattaaa gcggtgttgc
    25201 tgcgttcgat tgagcagggt ggtacaacgc tgaaagattt tctgcaaagt gatggtaaac
    25261 cgggctattt cgctcaggaa ttgcaggttt acgggcgaaa aggtgagccg tgtcgggtgt
    25321 gcggtacgcc gattatggcg actaaacatg cgcagcgggc aacgttttat tgtcggcagt
    25381 gccagaagta attcatgcgc gccggatggc ataccatccg gcataaacgc tacgctaact
    25441 tcgccatcag cgcctgatgg acattctccg gcaggaaatg ggtgacatcg ccctgatggc
    25501 gcgccacctc tttaaccagg gatgaagaga taaacgacca ctctttcgac ggcataagaa
    25561 acacactttc cagttccggc attaagtggc gattcatatg cgccagctgc atttcatatt
    25621 caaaatccgc caccgcacgc aggccacgaa tcagcaccgt agcgtgttga ttacgggcga
    25681 agttcgccat caaatcacta aacccgacca cttccacgtt ccccagatgc gcggttgcct
    25741 gctgtgccag cgccacacgc tcttccagag taaacatcgg ttttttactg ggactggcgg
    25801 caatcgccag aataacgtga tcgaacattt gcgtggcgcg cgtcacgata tcgatatgac
    25861 cattggtaat gggatcgaaa gtacccggat aaatcgcccg tttttgcata acaacctcaa
    25921 tgcgtcttcg gtggcaggta aggttccagc agttgaagca gacgctgtag cgcgccctgg
    25981 ttttgataca gtacttcaac ggcatgacgg ccatagaaac tacggtaatc ggcgtcggtg
    26041 agtaaagagg aaacctcttt tgcaagcgta gtggcatcgg taacggtaat cagcccgctt
    26101 gcctgctcca gccgcgcgca aatgtcttta aagttaaacg tatgcggccc catcaatacc
    26161 ggaatagcgt gtgcggcagc ttccagcgga ttatgcccac cacgttcaac cagtgaaccg
    26221 ccaacaaagg cgagatcggc aatgccgtac agtaacatca actcgcccat tgtatcgcca
    26281 accacaacct gcgtgctggt ggaggggact tcccctgaag atcgtgtgat atagcttagt
    26341 ccagcctggc ggacaaggtt aatcgcatcc gggaagcgtt ctggatgacg gggtaccagg
    26401 atgagcaata aattcgggaa ttgctgtaac aatgcctgat gtgcggcgat caccacactc
    26461 tcttcgcctt catgagtgct ggtggcaatc cataccgggc ggtgtggtgc ccactggcgg
    26521 cgcagcgtca cggctttagc tgccaactgc ggcgttacag aaatatcgaa tttcaggcta
    26581 ccggtaacgg tcacctgatt attttttgcg cccagcgcca caaaacgcgc gccatcttct
    26641 tcattttgcg cagcaatcag tgtaatacga cgcagcaagc ggcggacgaa tttacccagt
    26701 ttggcgtaac ctgcggccga gcgggcagag agtcgcgcgt tagcgatcac cagcggaatt
    26761 ttacgtttat gtagcgccgc aatcaggtta ggccatagtt cggtttccat aatcaacacc
    26821 agtttggggt cgactttatt caggaaacgg ttgagtgcat cgggcagatc atacggcaga
    26881 taaacgtgct gaacatcctt cccgaaagcc gattgtacgc gctccgaacc ggttggcgtc
    26941 atggttgtaa cggtgatcgg taaatcagga taacgatgac gcagcgcgcg caccaacggg
    27001 atcgccgcca gagtttcacc gacggagacg gagtgcagca tgatgccgcc tggttttagc
    27061 ggatggcggt aaaaaccgta acgttcaccc cagcgttttc gataggccgg agccttacgt
    27121 ccgcgcaccc agagccgtat ccagatcagc ggctgaataa ggtagagaag ggcggtgtaa
    27181 agcaattcga gcatagtaaa tagctgactt atggatgtgc tggggattct atgtatttag
    27241 ctgtggcttt accattactt ttcccgtttt tgacttaaat agcaatttac ccgccaattc
    27301 ttgctgaaca tctatacttt ttttgtattt ttatgcgatt cattgatttc tgctgtacgt
    27361 gcaggaagga atcgcagagc aacagtgaaa gaatatggtt gctaatgatt ttatttgatt
    27421 gatttcctta ttgaaaatca acaatgtgct taaaaatcat tcgggtcgga ttgccgcgaa
    27481 agaagtgata cactagcgcg tcaatatcag tgcggtatca gtattcaggt agctgttgag
    27541 cctggggcgg tagcgtgctt tttctgcttc accttaatcg gataatctca acaaaaagag
    27601 ttacttgtgg ataagccatt tcgaagaatt ttgctcatta agatgcgttt tcatggggat
    27661 atgttattaa ctactcccgt cattagttcg ctgaaaaaaa attaccctga cgcaaaaatc
    27721 gatgtgctgc tttatcagga caccatcccg atcctgtctg aaaatccaga gattaacgcg
    27781 ctctacggca taaaaaataa aaaagcaaaa tcctcagaaa aaattgccaa cttttttcat
    27841 ctcatcaagg tattacgtgc caataagtat gaccttatcg ttaatcttac cgatcaatgg
    27901 atggttgcta tactggttcg cttattaaat gcccgtgtga aaatttccca ggattatcat
    27961 catcggcagt ctgctttttg gcgtaatagt tacactcatt tggtgccgtt gcagggtgga
    28021 aatgtggtgg aaagtaactt atccgtgctg tcgccattgg gacttgaatc gttggtgaag
    28081 cagacaacca tgagttaccc gcctgcaagc tggaaacgta tgcgtcgcga acttgatcac
    28141 gctggtgttg gacaaaatta tgtggttatc caacctacgg cgcggcaaat cttcaaatgt
    28201 tgggacaacg ccaagttttc cgctgtgatt gatgccttac atgctcgtgg ttatgaagtc
    28261 gttctgacgt ccggcccaga taaagacgat ctggcctgcg tcaatgaaat tgcccaggga
    28321 tgccagacgc caccagtaac ggcgctggcc ggaaaggtga ccttcccgga acttggtgcg
    28381 ttaatcgatc atgcgcagct gtttattggc gttgattccg caccggcgca tattgccgct
    28441 gcagttaata cgcctctgat atcgctgttt ggtgcgacag atcatatttt ctggcgtccc
    28501 tggtcaaata acatgattca attctgggcg ggagattacc gggaaatgcc aacgcgcgat
    28561 cagcgtgacc gaaatgagat gtatctttcg gttattccgg cggcagatgt cattgctgct
    28621 gtcgataaat tactgccctc ctccacgaca ggtacgtcgt tatgatcgtt gctttttgtt
    28681 tatataaata ttttcccttt ggcggtttgc agcgcgattt tatgcgtatt gctcagacag
    28741 tcgccgcccg aggtcatcat gttcgggttt atgcccagtc gtgggaaggc gaatgccctg
    28801 atgtatttga actgatcaaa gtgccggtta aatcgcatac caatcacggg cgcaatgcgg
    28861 agtattttgc ctgggtgcaa aaacatttac gtgaacatcc cgtcgataga gtcgttggat
    28921 tcaacaaaat gccggggctg gacgtttatt atgccgctga tgtttgttat gccgagaaag
    28981 tagcgcagga gaaaggcttt ttctatcgcc tgacgtcacg ttatcgccat tatgccgcct
    29041 ttgagcgggc aaccttcgaa cagggcaagc cgacacagct gctgatgctg acagataagc
    29101 aaatcgccga tttccagaaa cattatcaga ctgaagcgga gcgttttcat attctgccac
    29161 cggggattta tcctgatcgt aaatatagcc agcagccagc caatagccgt gaaatcttcc
    29221 gtaagaagaa tggaataacc gaacaacaat atttattgtt gcaggtcggt tcagacttca
    29281 cgcgtaaagg tgtcgatcgt tccattgaag cacttgcttc attacctgat tcgctgcgcc
    29341 acaacacatt gctatatgtt gttgggcagg ataaaccgcg aaaatttgag gcgctggcag
    29401 aaaaacgcgg cgtgcgcagt aatgttcact tcttctcggg gcgcaacgat gtctcggaat
    29461 taatggcggc ggcggattta ttactgcatc ctgcctacca ggaagcggcg ggaattgtgc
    29521 tgctggaagc gattactgca ggattaccgg tactaacaac agccgtttgt ggctatgcgc
    29581 attatattgt tgacgccaat tgcggcgagg ctattgctga gccattccgc caggaaacat
    29641 tgaatgagat tttacgcaaa gcgttaacgc aatcttcatt gcgccaggct tgggcggaaa
    29701 atgcgcgaca ttatgctgat acacaagatt tatacagtct gccagagaaa gcggcggata
    29761 tcataacggg tggtctggat ggttgaactt aaagagccgt ttgccacgtt atggcgcggt
    29821 aaagatcctt ttgaggaagt taaaaccttg cagggtgagg tatttcgtga actggaaact
    29881 cgccgcactc tgcgctttga aatggcgggc aaaagctatt ttctcaaatg gcatcgcggc
    29941 acgaccctga aagagataat caaaaattta ctctcattgc ggatgccagt attaggcgca
    30001 gaccgcgaat ggaatgcgat tcatcgactg cgggatgtcg gcgttgatac tatgtatggg
    30061 gtggcattcg gcgaaaaagg catgaatccg ctgaccagaa cctcgtttat tattaccgaa
    30121 gatctgacac caaccatcag tctggaagat tactgtgctg actgggcgac caacccacca
    30181 gatgttcgcg taaagcgtat gcttattaag cgtgtcgcga tgatggtgcg cgatatgcat
    30241 gctgcgggca ttaaccaccg tgactgttat atctgtcatt tcctgctgca cttgcctttt
    30301 tccggtaagg aagaggagtt aaaaatttcg gtaattgacc tgcaccgagc gcagcttcgc
    30361 acgcgcgttc cacgtcgttg gcgcgataaa gatcttattg ggctttattt ttcttcgatg
    30421 aatattggcc tgactcagcg ggatatctgg cggttcatga aggtgtattt tgctgccccg
    30481 cttaaagaca ttctcaagca ggaacaaggg ctgctgtcgc aagcagaagc aaaagccaca
    30541 aaaatcaggg aaagaacgat tcgaaaatcg ttgtaacccg taacttattt ttgccaaaaa
    30601 tttggataca gagtaaatat gtctcaa
//
LOCUS       DAPHBJ010000033        30147 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.033, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000033 DAPHBJ010000000
VERSION     DAPHBJ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.033"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="RHZ76_001391"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="RHZ76_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDV2812053.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="RHZ76_001392"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="RHZ76_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDV2812054.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="RHZ76_001393"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="RHZ76_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDV2812055.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="RHZ76_001394"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="RHZ76_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDV2812056.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="RHZ76_001395"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="RHZ76_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDV2812057.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="RHZ76_001396"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="RHZ76_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDV2812058.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="RHZ76_001397"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="RHZ76_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDV2812059.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="RHZ76_001398"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="RHZ76_001398"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDV2812060.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="RHZ76_001399"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="RHZ76_001399"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDV2812061.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12906..14051)
                     /gene="thiH"
                     /locus_tag="RHZ76_001400"
     CDS             complement(12906..14051)
                     /gene="thiH"
                     /locus_tag="RHZ76_001400"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDV2812062.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDSYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRLLDGM"
     gene            complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="RHZ76_001401"
     CDS             complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="RHZ76_001401"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDV2812063.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="RHZ76_001402"
     CDS             complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="RHZ76_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDV2812064.1"
                     /translation="MQILFNDQAMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="RHZ76_001403"
     CDS             complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="RHZ76_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HDV2812065.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="RHZ76_001404"
     CDS             complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="RHZ76_001404"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDV2812066.1"
                     /translation="MYQPDFPPVPFRSGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="RHZ76_001405"
     CDS             complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="RHZ76_001405"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDV2812067.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18363..18463)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18515..18991)
                     /gene="rsd"
                     /locus_tag="RHZ76_001406"
     CDS             complement(18515..18991)
                     /gene="rsd"
                     /locus_tag="RHZ76_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HDV2812068.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19086..19859
                     /gene="nudC"
                     /locus_tag="RHZ76_001407"
     CDS             19086..19859
                     /gene="nudC"
                     /locus_tag="RHZ76_001407"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HDV2812069.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19899..20963
                     /gene="hemE"
                     /locus_tag="RHZ76_001408"
     CDS             19899..20963
                     /gene="hemE"
                     /locus_tag="RHZ76_001408"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDV2812070.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20973..21644
                     /gene="nfi"
                     /locus_tag="RHZ76_001409"
     CDS             20973..21644
                     /gene="nfi"
                     /locus_tag="RHZ76_001409"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HDV2812071.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21687..22277
                     /locus_tag="RHZ76_001410"
     CDS             21687..22277
                     /locus_tag="RHZ76_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HDV2812072.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22464..22736
                     /gene="hupA"
                     /locus_tag="RHZ76_001411"
     CDS             22464..22736
                     /gene="hupA"
                     /locus_tag="RHZ76_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HDV2812073.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22749..23444
                     /locus_tag="RHZ76_001412"
     CDS             22749..23444
                     /locus_tag="RHZ76_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HDV2812074.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23446..23871)
                     /gene="zraP"
                     /locus_tag="RHZ76_001413"
     CDS             complement(23446..23871)
                     /gene="zraP"
                     /locus_tag="RHZ76_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HDV2812075.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24109..25485
                     /gene="zraS"
                     /locus_tag="RHZ76_001414"
     CDS             24109..25485
                     /gene="zraS"
                     /locus_tag="RHZ76_001414"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HDV2812076.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGNQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25482..26807
                     /gene="zraR"
                     /locus_tag="RHZ76_001415"
     CDS             25482..26807
                     /gene="zraR"
                     /locus_tag="RHZ76_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HDV2812077.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQIISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26804..28093)
                     /gene="purD"
                     /locus_tag="RHZ76_001416"
     CDS             complement(26804..28093)
                     /gene="purD"
                     /locus_tag="RHZ76_001416"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDV2812078.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACESKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADDEQVVTNGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28105..29694)
                     /gene="purH"
                     /locus_tag="RHZ76_001417"
     CDS             complement(28105..29694)
                     /gene="purH"
                     /locus_tag="RHZ76_001417"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDV2812079.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKLDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPSEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7055 a         7862 c         8300 g         6930 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctgcgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagacccc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaat gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacgc cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac gtataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgtc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgtt tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggcaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaag ctctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttagtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggaactg gtgccggaac gcctgcgtgg tgaaaccgca
     5101 tcttttgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ctggctaagc tgagccagtc tggtcacaag
     5341 cgtatcgaaa cgctgttcac caacgatctg gatcacggcc catatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagcg gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacctgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ccgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggat
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc caggcggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcact acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctc tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaagtga ccgacggtgt tgtaactgac gaaattcact acctgtctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgtga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgtg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccaga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tatattggtg cggaagtgac cggtggcgac attctggttg gtaaggttac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtctg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgaccgacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtatatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcgggttaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agttgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gttggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccgctggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 tgacgccctg ctggataacg gtcgtcgcgg tcgtgcaatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctact
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc actggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gtactgggtc tgtactacat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcta acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtt attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg ttaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaata ccgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtaccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 ccagaccatt acgcgtcaga ccgacgaatt gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga tcccaggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 tctgttcgaa gcacgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtta ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gtagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagacgactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggccg gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatgggtg ttattcagag ggcaaattct tcacggaaaa tattaagaaa ggcagggcga
    12781 aacgaggaag aagcttttcg ccctggacaa cattcctgct gacggcacta ccataaaaaa
    12841 acacaacgct ttcaacaacc ttgcgttttt catcttccgt agcggctcgg aataaattta
    12901 cgttcttaca ttccgtctaa tagtctttgc gaggcgcgtc ccagatagct gtcccagtct
    12961 ttccataccg gctgcaaacc ctgagcggtt aacgcggcag caaccgcttc cggtctgcga
    13021 tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat cggcatagcc acctggctgc
    13081 gttttcgaga aggcgctgac gttattgatc gccagcggaa taacgcgatc gcgaaaccac
    13141 ggtgattccc gcgtggagag tgacagttca atctccggtg caagcagtcg gaaggcgcag
    13201 atggtttgca ctaactggcg ttcatccata atcgacgcag gctcaatgcc gccagtacac
    13261 gggcgcaggc gcggaaagga gacagagtaa cggctttgcc agtaatgctg ttgcagccat
    13321 agcaaatgtt cggcaaccat atagctgtca acgcgccagt tgtcggaaag gccaattagc
    13381 gcgccgaggc ctatcttatc aatccccgca cgccccagcc gatccggcgt ttccagccgc
    13441 cagaagaagt cctgtttttt gcctttcaga tgatggcggg cataagtcgc ctcgtgatat
    13501 gtctcctgat aaaccatcac gccatccaga ccaagttgct ttaactcggc gtattccgtc
    13561 tccgccagcg gttgcacttc catctgtagt gaagagaact gttcacgaag ggcagggaga
    13621 tgacgacgaa agtaatccat ccccactttc gcctgatgtt caccagtgac taacagcaga
    13681 tgttcaaagc ccatctcccg tatagcggca ctttccctgg caatatccgc ttcatccagc
    13741 gttttgcgct tgatgcgatt actcatggaa aatccacagt acgtgcagtc gttagcgcaa
    13801 agattggaaa gataaagcgg gacgtagaaa ctaactgtgt tgccaaatcg ctgacgggtc
    13861 agacgctgcg cccgttgggc cagttgttcc agatagccac tggcggcagg cgataacagc
    13921 gccatcatgt cgtcgcgggt gagttgcgag gcatttagcg cccgctctac gtcagcagcc
    13981 gttttgccgt tgatacgcag gcggatgtcg tcccagtcca gttgtcgcca gcgatcgctg
    14041 aaggttttca tgccgatgcc tccagaaatc cggtcagcgg gctggtggca tgagcaaaat
    14101 aactgcggct gcccggtccg gactgacgtg ccagtaggcc tgcttctacc gccagacgaa
    14161 atgccttcgc catgttgacg ggatcgtccg cgacggcaat cgccgtattc actaacaccg
    14221 cgtcggcccc catttccagc gcctgcgcgg catggctggg aacgccgatg ccagcatcga
    14281 caaccaccgg cactgtggcc tgctggataa taatctccag catggcgcgg gtttccagtc
    14341 cctgattcga gccaatcggc gcgccgagcg gcatcaccgc tgcacagccg acttcttcca
    14401 gacgtttaca caataccgga tcggccccgc agtaaggcag cacgacaaat ccctgttgta
    14461 ccagcgtttc ggcggctttc agggtttcga tgggatcggg caacagccag cgggcgtcag
    14521 ggtgaatctc taattttaac cagtttgtgc ctaacgcttc acgagccaga tgggcggcga
    14581 aaatggcttc ttccgctgtt ttcgccccgg atgtatttgg cagcagggtc acacccgccg
    14641 cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa gtcgacacgt ttcatcgcca
    14701 gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac catcagttgt gaagaagcga
    14761 atttccctgt gccggtaaac agatgtgaat caaacgtttt gtccgcaata cgtaacattt
    14821 caaccccctg caataacctg aaaaagcagg atctggtcgc catcctgcac gatatgttgc
    14881 gcccactgct cacgcgggac gatttgctga ttaatcgcca gagccgcgcc cgcttgtcgt
    14941 tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg cggcgcactg catcgcttga
    15001 tcgttaaaca ggatctgcat tgcttcctcc gcataccggg caaccactgg cgcggcgcaa
    15061 cgccaggctg cgccactggc tcgatttacc gtcgaacagt cggagttctc ccgcaggtgt
    15121 ctctataccg cttaataact taatggcttc cagtgcctgc aaagtgccca taaccccgac
    15181 caccgggcca accacgcccg ccgtgcggca gttgcgttct ggctcctggt tatctggcca
    15241 caggcagcgg taacacccct gctcccaggg cggcgtcagt accatcaact gaccgccaaa
    15301 tccgaccgcg ctggcggtga taagcggcgt gttgagtgcc acgcaggcgg cattaatctc
    15361 ctggcgagtc gccatattgt cggtacagtc gagcaccaca tcggcccgtg caaccgcatc
    15421 ttttaacgcc tcacccgtta accgttgttg taatgctgtc agttgaatgt cgggattcaa
    15481 ctgtgtcagt cgctgttggc tgacctgcga tttcgggcga tcgatatctt cagtggtaaa
    15541 gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg tcatctgcca gtaccagcgt
    15601 cccgacgcca gcgcccgcca ggtacagcgc agcaggtgta cccagcccgc ccagaccgat
    15661 aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc agagcgatat cgtcgagcag
    15721 gatttggcgg ctataacgca taaagtcacg gtcattcatc gccaactcct gcaatttcca
    15781 gcaactgtgc cgttgccaaa cgccagtctg cggcttgagt aatggcgctg acgacggcga
    15841 tactgccgac acccgttgct atcaccgcag gcgcgcgtgc cagactgata ccgccaatcg
    15901 ccacggtggg ataatccgcc agtcgctcaa catgccgtgc cagctgttcc agcccctgcg
    15961 gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg tcccagcgcg atataagagg
    16021 ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg tgtcgaaacg cccagccgca
    16081 ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa atcttcctgc cccaaatgga
    16141 cgccatacgc ctgatgcttg atcgccagcc gccagtaatc gttgataaac aatcgcgcgt
    16201 tatagcggcg gcccagcgca attgccgcca cgacatcggc ttccacctct tcatcgcgcc
    16261 gatctttgat gcgtagctgg agagtacgta cgcctgcatc caacagacgt tcgatccact
    16321 gtacgctgtc caccaccggg tacagtcctg aacgaaaagg tacaggagga aaatcaggct
    16381 gatacatcac gcttcctcct tacgcaggta gatttctccg cctctggcac ggaagttctc
    16441 cgacatatcc gccattccca tttcaatagt ttgcgtggcg gcgtaatcac gcacttcctg
    16501 gctgattttc atcgagcaga atttcggccc acacatggag caaaaatggg cgactttacc
    16561 tgactcttgc ggcagggttt catcgtgata agcgcgggcg gtaaacgggt cgagggccag
    16621 attaaactgg tcttcccagc gaaattcgaa gcgggctttc gacatggcgt tatcgcgaat
    16681 ttgcgcgccc ggatgccctt tcgccaggtc agcggcgtgg gcggcaatct tataggtgat
    16741 aagcccctgc ttaacatctt ctttattggg cagacccaga tgctcttttg gcgttacgta
    16801 acagagcatc gcgcagccaa accagccaat catcgccgca ccaatccccg acgtgaagtg
    16861 gtcatagccc ggcgcaatat cggtagttag cggccccaga gtgtaaaacg gcgcttcgtg
    16921 gcagtgctct aactcctcgg tcatattgcg gcggatcatc tgcatcggca cgtggcctgg
    16981 gccttcaatc atcacctgca cgtcatattc ccaggcaatt ttggtcagtt cgcccagcgt
    17041 atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga atagaaccgg ggcgcagacc
    17101 gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt tcacaaattt cgcggaagtg
    17161 ttgatagagg aaattttcct gatgatggga gaggcaccat ttcgccataa tcgaaccgcc
    17221 gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc acatagcgca gcagtacgcc
    17281 cgcatggata gtgaagtaat ccacaccttg ctcggcctgt tccagcagcg tgtcgcggaa
    17341 cgcttcccag gtaagatctt cggcgatccc gttaaccttc tccagcgctt ggtagatcgg
    17401 cactgtaccg atcggcaccg ggctgttacg caaaatccac tcgcgggttt cgtgaatata
    17461 gcgaccggtg gagagatcca tcaccgtatc cgctccccag cgggtggacc ataccagctt
    17521 ttccacttct tcttcgatgg aagaggtgac cgccgagttg ccgatattgg cgttaacttt
    17581 taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc ggatgattaa tgttggccgg
    17641 gataatcgca cgtccggcag caacttcatc acggacaaat tccgcagtga tattttccgg
    17701 cagatgtgcg ccaaagctca ttcccggatg ctggtggcgt aaaacctcgc tacggatgcg
    17761 ctcgcggccc atattctcgc ggatggcgat gaattccatt tccggcgtga tgatgccctg
    17821 gcgggcgtag tgcagttggg tgacacggcg tcctgctttg gcgcgttttg gtgttagtac
    17881 gccgctaaaa cgcagttcgt cgaggccatc atctgccagc cgcgctttag tgtaatcgga
    17941 actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg atccacggct ggcgtagttt
    18001 tgccagccct tgctgcacgt taatggcaat ctgcggatca ccatacgggc cggaggtgtc
    18061 gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt tctttgctac cgccaattag
    18121 cgtcgggcta agctggatct cacgcatcgg cacgcgcacg ccggggtgtg tgccagtgat
    18181 ataaatgcgt tttgagttgg gaaaggcggt gccttccagg gtgtcgataa aatgttgggc
    18241 ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac atagctcatt ccaaaaagtt
    18301 aaggacgtgg cttgtcagac gacggatgaa gcaagagacg atcgccgcag gggcgatgcg
    18361 atagcagatt aactcttgtt cccttcgcag gtattagcct gatcaggttc cgcggatccc
    18421 gaataaacgg tctcagccag ttaaggcact ccgacaagaa attagccccg caaatggggc
    18481 attgaatgta aattacgcgt taacagcgca gaactcaagc aggatgtttg acgcgggcgg
    18541 cgtcaagcac cagcagaatc agcttatctt ccagcacaaa gcgcgcttcc agcgcctcgc
    18601 cgatgtcaga taaaacctgt tgaaactcaa ggtaattatc atgatcgatg gcggtttcca
    18661 gactggaatc gtagtaatcc ataatctgtt gagtattggc ttcgagttgc ggccaaatct
    18721 tcgcggcgcg cgcgagttgc ccgttgcctt ccagcttatg aagaatgcgc tcataaatac
    18781 tgaaatgtcc ggcagacaag taatcgacca ggctctgaca aaaatcatca agggcttttt
    18841 cgtttagcct catgtacgat tctttgccag gcttaatgcc aaccagattg tagtaagcca
    18901 cgagcagatg cttacgtaca tgtagccagc gatcaaccag tttgttactt cctctgacgc
    18961 gctccgtcag gttatcgagc tggttaagca tgattgactc cgcaagtttg tattcaaaaa
    19021 ctgctcagtg agaaatgtaa aaaccatgtt aaacatgcca gtgatgcaaa ggtagtgcaa
    19081 gagctatgga tcgtataatt gaaaaattag atcacggctg gtgggtcgtc agccatgaac
    19141 aaaaattatg gttgccgaag ggagaattgc catatggcga agcggcaaat ttcgatcttg
    19201 tgggtcagcg cgcactacaa atcggcgaat ggcaggggga acctgtttgg ttagtacaac
    19261 agcagcggcg tcacgatatg gggtcggtac gtcaggtcat tgatctcgat gttgggctgt
    19321 ttcaactggc cggacgaggc gtacaactgg cggagtttta ccgatcgcat aaatactgtg
    19381 gttactgcgg gcatgaaatg tatccgagca aaaccgaatg ggcgatgctg tgcagccatt
    19441 gccgtgagcg ttactacccg caaatcgccc cctgcattat tgttgccatc cgtcgcgatg
    19501 attcgatcct cctcgcccag catacccgcc atcgtaacgg tgtccataca gtacttgccg
    19561 gattcgtcga agtgggcgaa accctcgagc aggcagtcgc gcgggaagtg atggaagaga
    19621 gcggaattaa agttaaaaac ttgcgttacg tgacttctca gccgtggccg tttcctcagt
    19681 ctttaatgac cgcgtttatg gcggaatatg acagcggcga catcgtgatc gacccgaaag
    19741 aattgctcga ggcgaactgg tatcgctatg acgatttgcc gttactcccg ccgcccggca
    19801 ccgtagcgcg ccgtctgata gaagatacgg tggcgatgtg tcgggcagag tatgagtgat
    19861 gatacactga ccgcctgacg cactaaggaa cagccaaaat gaccgaactt aaaaacgatc
    19921 gttatctgcg ggcgctgctg cgccagcccg ttgatgtcac tccagtatgg atgatgcgcc
    19981 aggcgggtcg ctatctaccg gaatataaag ccacgcgcgc ccaggcaggc gattttatgt
    20041 cgctgtgcaa aaacgccgag ctggcgtgcg aagtgacttt gcaaccgctg cgtcgctacc
    20101 cgctggatgc ggcgatcctc ttttccgata tcctcaccgt gccggacgcg atggggttag
    20161 ggctctattt tgaagccgga gaaggtccgc gttttacctc gccagtcacc tgcaaagctg
    20221 acgtcgataa actgccaatt ccggacccgg aagatgaact gggttacgtg atgaacgcgg
    20281 tgcgtaccat tcgtcgcgaa ctgaaaggcg aagtgccgct gattggtttt tccggcagcc
    20341 cgtggacgct ggcgacctac atggtggaag gcggcagcag caaagccttc accgtgatca
    20401 aaaaaatgat gtatgccgat ccgcaggcgc tgcacgctct gctcgataaa ctggcgaaaa
    20461 gcgtcacttt gtatctgaat gcgcagatta aagccggtgc tcaggcagtg atgattttcg
    20521 acacctgggg cggcgtgctt accgggcgcg attatcaaca gttctcgctc tattacatgc
    20581 ataaaattgt tgatggttta ctgcgtgaaa acgacggtcg ccgcgtaccg gtcacgctgt
    20641 ttaccaaagg cggcggacag tggctggaag ccatggcaga aaccggttgc gatgcgctgg
    20701 gcctcgactg gacaacggac atcgccgatg cgcgccgccg tgtgggcaat aaagtcgcgt
    20761 tgcagggtaa tatggatccg tcgatgctgt acgcgccgcc tgcccgcatt gaagaagaag
    20821 tagcgactat acttgcaggt ttcggtcacg gcgaaggtca tgtctttaac cttggtcacg
    20881 gcattcatca ggatgtgccg ccagaacatg ctggcgtgtt cgtggaggca gtgcatcgac
    20941 tgtctgaaca gtatcaccgc taaggagtga ttatggatct cgcgtcatta cgcgctcaac
    21001 aaatcgaact ggcttcttct gtgatccgcg aggatcgact cgataaagat ccaccggatc
    21061 tgatcgccgg agccgatgtc gggtttgagc agggcggaga agtgacgcga gcggcgatgg
    21121 tgctgctgaa atatccctcg cttgagctgg tcgagtataa agttgcccgc atcgccacca
    21181 ccatgcctta cattccaggt tttctttcct tccgcgaata tcctgcgctg ctggcagcgt
    21241 gggagatgct gtcgcaaaag ccggatttag tgtttgtcga tggtcatggg atctcgcatc
    21301 ctcgccgtct tggcgtcgcc agccattttg gcttattggt ggatgtgccg accattggcg
    21361 tggcgaaaaa acggctctgc ggtaaattcg aaccgctctc cagcgaaccg ggcgcgctgg
    21421 ccccactgat ggataaaggc gagcagctgg cctgggtctg gcgcagcaaa gcgcgctgta
    21481 acccgttgtt tatcgctacc ggccatcggg tcagcgtgga cagcgcgctg gcgtgggtac
    21541 aacgctgcat gaaaggctat cgtctgccgg agccaacgcg ctgggcggac gcggtggcct
    21601 cggaacgtcc ggcgttcgtg cgctatacag caaatcagcc ctaattcagg taaactgcgg
    21661 ataatttccg tatttgagaa ctcaacatgt tacaaaaccc aattcatctg cgtctggagc
    21721 gcctggaaag ctggcagcat gtcactttca tggcttgctt atgcgaacgc atgtacccca
    21781 attacgccat gttctgccag caaaccggtt ttggtgatgg gcaaatttac cgtcgcattc
    21841 tcgatctcat ctgggaaacg ctgaccgtta aagacgcaaa agtaaatttc gacagccaac
    21901 tggagaaatt tgaagaagcg attccttcag ccgacgattt cgatctgtac ggcgtttatc
    21961 cggcaatcga tgcctgcgtg gcgttaagtg aactggtcca ttcgcgtttg agtggtgaaa
    22021 cgctcgaaca cgcggtggaa gtgagtaaga cctccatcac gaccgttgcg atgctggaaa
    22081 tgactcaggc tggtcgcgaa atgagcgatg aagagcttaa agaaaaccca gctgtagagc
    22141 aagaatggga cattcagtgg gaaatattcc gacttttagc tgagtgcgaa gaacgcgata
    22201 tcgagctgat aaaaggcctt agggcagacc tgcgtgaggc gggtgagagc aatattggta
    22261 taatttttca gcaataagac cagaaaacgt gatttaacgc ctgatttgtc gtacctggag
    22321 tcttcccttt cgccccccgt ctggtctaca tttggggggc gaaaaaaagt ggctatcggt
    22381 gcgtgtatgc aggagagtgc tattctggca tttccgtcgc actcgatgct tagcaagcga
    22441 taaacacatt gtaaggataa cttatgaaca agactcaact gattgatgta attgcagaga
    22501 aagcagaact gtccaaaacc caggctaaag ctgctctgga gtccactctg gctgcaatta
    22561 ctgagtctct gaaagaaggc gatgctgtac aactggttgg tttcggtacc ttcaaagtga
    22621 accaccgcgc tgagcgtact ggccgcaacc cgcagaccgg taaagaaatc aaaattgccg
    22681 cagctaacgt accggcattt gtttctggca aggcactgaa agacgcagtt aagtaagatt
    22741 gcgtggcagt gaacagtttt aacgaagggg tggtttcacc ccttttgtct ttctggcgtc
    22801 gatcattgat gctggctggc gctctgcttc tcactgcctg tagtcataac tcttcacttc
    22861 ctccctttac cgccagtgga tttgctgaag accagggcgc ggtacgcatc tggcgaaaag
    22921 acagcggcga taatgtgcat ctgcttgccg tgtttagccc gtggcgcagt ggcgatacca
    22981 cgacgcgaga gtatcgctgg cagggcgata acctcacgct catcaatatc aatgtttaca
    23041 gcaaaccgcc ggtgaatatt cgcgcgcgtt ttgacgatcg cggtgatctg agctttatgc
    23101 aacgtgaatc cgatggggaa aagcagcagc tttctaacga ccaaatcgat ttataccgtt
    23161 atcgtgctga tcagatccgc cagattagcg atgccttacg tcaggggaga gtcgtgctgc
    23221 gccaggggcg ctggcatgcg atggaacaga ccgtgaccac ctgcgaaggg caaaccatta
    23281 aacctgattt agattcgcag gcgatagcgc atatcgagcg ccgccagagc cgctcttctg
    23341 ttgatgtcag cgtggcatgg ctggaagcgc ccgaaggttc gcaattactg ttagtggcaa
    23401 actctgattt ctgtcgctgg caacccaacg agaaaacgtt ctgatttacc agtggcccat
    23461 acccatatga ccgccaccac cgcagccgcc gtagcccatg cccattccgg ctccgcgcgg
    23521 aatacccgct tcagccatcg cgatatctcg tttcacccgt aactcatcta acgactgacg
    23581 caaattctcc atctctttgg cgaccgcgtt aattttgctg ctatccggtg ggttcgcggc
    23641 taacagggca ttgtattcat aacgcttcgt caccagttgc tgttgcagtg cgctgctttg
    23701 agcgtaaaag tcattatgga ttttctgcca cgctgtctgt tgttcgctgg tcaaaggcgc
    23761 ggcattttgc tgccacatac cgtgtccgcc gtgagcaaat gcagatgtcg atcccatcgc
    23821 cattgctgaa agcgccatca ttaccagggc aattttcgtg ttccgtttca tggttaatcc
    23881 tccagtggtt gtcttacttc gggtattgca tcttccgtgc caacgatgaa acgctgatat
    23941 gacgggtaat ctggcatgat aaacgagtaa aaatgactcg cctgctgcgg gtagcgagtc
    24001 atttttactc attgaaactt aagcctttgt gttacagcgc agggtaagcg ctgataaaag
    24061 atggcatgat ttctgctgtc agaaagggat gagcaggcaa agaagaagat gcgttttatg
    24121 caacgttcta aagactcctt agctaaatgg ttaagcgcga tcctccccgt ggtcattgtt
    24181 gggctggtag ggttgtttgc ggtgacggtg attcgcgatt atgggcgcga gactgccgcc
    24241 gccagacaaa cgctgctgga aaaaggcagt gtacttattc gcgctcttga atccggctcg
    24301 cgcgtcggca tggggatgcg catgcatcat gcgcagcagc aggccttact ggaagaaatg
    24361 gccgggcagc ctggagtacg ttggtttgcg gtcacggatg aacaaggaac aatcgtgatg
    24421 catagcaact ccggcatggt gggaaatcag ctttattccc ctcaggaaat gcagcagtta
    24481 catccgggag atgaagaagc gtggcggcgg atcgatagcg cagacggcga gcctgttctg
    24541 gaaatttatc gccagtttca accgatgttt gctgctggaa tgcaccggat gcgccatatg
    24601 cagcagtatg ccgcgacacc acaagcaatt ttcatcgctt tcgacgccag taatattgtg
    24661 agtgccgaag atcgtgagca gagaaacacc ctgattattc tcttcgccct ggcgacggtc
    24721 ttgctggcaa gcgtattgtc attcttctgg tatcgccgct atctgcgctc gcgccagctt
    24781 ctacaagatg aaatgaagcg caaagagaag ctggtggcgc tggggcatct tgcggcaggc
    24841 gttgcccacg aaatccgtaa cccactttcc tcgattaaag gactggcgaa atactttgcc
    24901 gagcgcgcgc ctgcaggggg agaagcgcat caactggcgc aggtgatggc gaaagaggcc
    24961 gaccgtttaa accgcgtggt aagcgagttg ctggaactgg ttaagccaac gcatctggct
    25021 ttgcaggcgg tggatctcaa cacgctgatt aaccactcat tacagctggt aagccaggat
    25081 gcaaacagcc gggagatcca gttacgcttt accgccaacg acacattacc ggaaattcag
    25141 gccgatccgg acaggctgac tcaggtcctg ttgaatctct atctcaatgc tattcaggcg
    25201 attggtcagc atggcgtgat tagcgtgacg gtcagcgaaa gcggcgcggg cgtgaaaatc
    25261 agcgttaccg acagcggtaa gggaattgcg gcagatcagc ttgaagccat cttcactccg
    25321 tacttcacca ccaaagccga aggcaccgga ttggggctgg cggtcgtgca taatattgtt
    25381 gaacaacacg gtggtacaat tcaggtcgca agccaggagg gaaaaggctc aacgttcacc
    25441 ctctggcttc cggtcaatat tacgcgtaag gacccacaag gatgacgcac gataatatcg
    25501 atattctggt ggtggatgat gacattagcc actgcactat tttgcaggct ttactgcgcg
    25561 gctggggcta taacgtcgcg ctggcgaaca gcgggcgaca ggcgctggag caggtgcggg
    25621 aacaggtttt tgatcttgtg ctttgcgatg tgcgaatggc ggagatggac ggcatcgcca
    25681 cgctgaaaga gatcaaagcg ttaaacccgg caattccggt gctgattatg actgcgtact
    25741 ccagcgtcga gacggcggta gaggcactga aaactggggc gctggattat ctcatcaagc
    25801 cgctggattt cgataaccta caggcgacgc tggaaaaagc gctcgcgcat acgcacagta
    25861 ttgatgctga aacacctgcg gtgactgcca gccagttcgg tatggtcggt aaaagcccgg
    25921 cgatgcaaca cctgctcagt gaaatcgccc tcgtcgcgcc atcggaagcc acggtactga
    25981 tccacggcga ttccggcacc ggtaaagagc tggtcgccag ggcgattcac gccagtagcg
    26041 cacgtagcga aaaaccgctg gtaacgctca actgtgcggc actcaacgaa tccttgctgg
    26101 aatctgaatt gttcggtcac gaaaaagggg cgtttactgg agccgataaa cgccgggaag
    26161 ggcgctttgt tgaggcggac ggcggcacgc tgtttctcga tgaaattggc gatatctcgc
    26221 cgatgatgca ggtgcgtctg ctgcgtgcga ttcaggagcg cgaagttcag cgtgtcggta
    26281 gcaaccagat tatctcggtt gatgtccggc tgattgcggc gacccatcgc gatcttgccg
    26341 cagaggtgaa tgccgggcgt tttcgccagg atctctacta tcgcctgaat gtggtggcga
    26401 ttgaagtacc atcgctgcgg caacggcggg aagatatccc tctgctggct ggccattttc
    26461 tgcagcgctt tgccgagcgt aatcgcaagg cggtaaaagg ttttacgccc caggcgatgg
    26521 atctgttgat tcattacgac tggccgggaa atattcgtga gctggaaaac gcggtggaac
    26581 gggcagtggt gctgctgacc ggggaatata tttccgaacg cgagctgccg ctggcgattg
    26641 ccagtacgcc gatcccgctg ggacaaagtc aggatattca gccgttggtg gaagtggaaa
    26701 aagaggtgat tctggcggcg ctggagaaaa cgggcggcaa caaaaccgaa gccgcccgtc
    26761 agttagggat cacgcgcaaa acgctattgg caaaactgtc gcgttagttc tgctcgcgtt
    26821 cgatagcgcg ccagccgata tctttccggc agaagcagtc gtcccagtgg atatcggtca
    26881 ttaaggcata ggcgcgtttc tgcgcttctg ccacggtatg acccagcgcg gtgacgcaca
    26941 gtacgcgccc gccgttggtc actacctgct cgtcatccgc cagttttgtg cccgcgtgga
    27001 acactttgcc gcctgccact tcttccagcg gcaggccgtg gatcacgtca ccggtgcggt
    27061 aatcacccgg atatccaccc gcagccatca ccacgccgag agaagcgcgt tcatcccact
    27121 cggacgtttt ctcgtccagt ttgctttcac aggccgctag gcagagctca accagatcgg
    27181 acttcatgcg cagcataatc ggctgggttt ccggatcgcc aaagcggcag ttaaattcaa
    27241 taaccttcgg attgccctgt ttgtcgatca tcaggcccgc gtagagaaaa ccggtgtagg
    27301 tgttgccttc cgccgccatg cctttcacgg ttggccagat aatacgttcc atggtgcgct
    27361 gatgaacgtc atcggttact accggcgcgg gggagtaagc gcccatcccg ccggtgtttg
    27421 gtccggtatc tttatcgcct acgcgtttgt gatcctggct ggtagccatc ggcagcacat
    27481 gctcgccgtc caccatcacg ataaagctcg cttcttcgcc atcgaggaac tcttcgataa
    27541 cgatgcgatg acccgcgtcg ccaaaagcgt tgcccgccag catatcgtga acagccgctt
    27601 ccgcttcttc cagcgtcatc gccacgataa cgcctttccc ggcagccaga ccgtccgctt
    27661 taatgacgat tggcgcgcct ttctcacgca gatacgccag cgcaggttct acctcggtga
    27721 agttctggta ttccgccgta gggatcttat ggcgggccag gaaatctttg gtaaacgctt
    27781 ttgagccttc cagttgggcc gcacctgcgg ttgggccgaa gattttcagc ccggcggcgc
    27841 ggaaggtatc gaccacgcct ttcaccagcg gcgcttccgg gccgacgatg gtcagatcaa
    27901 tcttttcgtt ttgtgcgaaa tccaacagcg ccgggatatc ggtcacgcca atagcaacgt
    27961 tttgcagcgc gggttccagt gcagtgcctg cattacccgg agcaacaaaa acagtctcaa
    28021 ccagcggcga ctgggccgct ttccaggcca gcgcgtgctc gcgcccgccg ttaccaatca
    28081 ctaatacttt catctattgc tccattaatg gcggaagtgg cgcatgtcgg tgaagagcat
    28141 cgcaataccg tgctcgtcgg cggcggcaat cacttcgtca tcacggatag aaccgccagg
    28201 ctggattacg caggtcacgc ccgcagcggc ggcggcatca ataccgtcgc ggaacgggaa
    28261 gaacgcgtca gaagccatcg aggaaccttt cacttccagg ccttcatcgg ccgctttaat
    28321 accggcgatt tttgcggagt acacgcggct catctggccc gcgccaatgc cgatagtcat
    28381 attgtttttg gcatagacga tagcgttgga tttcacaaac ttcgccacct tccagcagaa
    28441 cagcgcatca cgcagttcct gttcgctcgg ctgacgtttg gtcaccacgc gcagttcttc
    28501 cgcaccgacc atccccaggt cacgatcctg aaccagcaga ccgccgttca cgcgtttgaa
    28561 atcgaggccc ggaacacgct cgccccactg accgcaggtc agaacgcgta cgttctgttt
    28621 ggcggcggtg attttcaggg cttcttcgct ggcggacggc gcaataatca cttcgacaaa
    28681 ctgacgagaa atgatggcct gtgcggtttc cgcatccagc tcgcggttaa aggcaatgat
    28741 gccgccgaat gcggaggttg ggtcggtttt gtacgcgcga tcgtaagcat caagaatgga
    28801 attgccgata gccacgccgc aagggttggc gtgcttcaca atcacacatg ccggctcggc
    28861 gaactctttc acgcactcca gcgccgcatc ggtatcggcg atgttgttat aagagagggc
    28921 tttaccctga acctgggttg cggtagcaac ggaggcttct ttcacattct cttctatata
    28981 gaaggcagcc tgctggtggc tgttctcgcc gtaacgcata tccagcttct taatgaagtt
    29041 caggttcagc gtgcgtggga agcgaccggc ggcttctttg ctttcaccgt ggtaagccgg
    29101 aaccatgctg ccgaagtagt tggcaatcat gctgtcgtag gcggcagtgt gttcgaaggc
    29161 tttgatggcg aggtcgaaac gggttgcaag cgtcagcgat ccttcgttgt catccatctc
    29221 tttaataatg gcgtcatagt cgctgctctt caccacgatt gcgacatctt tatggttctt
    29281 ggcggcggag cgcaccatcg ttgggccgcc gatatcgatg ttctcaaccg catcttccag
    29341 cgagcaacct tcacgggcca cggtctgggc gaacggatac aggttaacaa ccaccatatc
    29401 gataggctgg atctgatgtt cttccataat ggcatcgtcc tggccgcgac ggcccagaat
    29461 gccaccatgt actttcggat gcagggtctt cacgcgtcca tccatcatct ccgggaaacc
    29521 ggtgtaatcg gaaacttcgg ttaccggcag acctttttct gctaacagac gggcagtgcc
    29581 ccctgtagac agcagctcca caccgcgtgc ggaaagtgcc tgggcgaatt cgacgatacc
    29641 ggctttgtca gaaacactga gcagagcgcg gcggactgga cgacgttgtt gcatggtaaa
    29701 tcccctggat ttgactatta cagagagcgt tagctgaatt tttcgcgaaa aactcagcta
    29761 acgcccctaa cggggcatcc ttatttttcg cccgcattgt aacgaaaacg tttgcgcaac
    29821 gctcgcgaat ttttctcttt caatggtgat cacaattttg acagtggtta ccgtgggaaa
    29881 aatacagaaa ttacattgat gattgtggat aactctgtgc gtaaaaaggt ataaagcggg
    29941 gttttgctgg ggaatgcagc agtcagttat ttttctgcaa tttttctatt gcggcctgcg
    30001 gagaactccc tataatgcgc ctccatcgac acggcggatg tgaatcactt cacacaaaca
    30061 gccggttcgg ttgaagagaa aaatcctgaa attcagggtt gactctgaaa gaggaaagcg
    30121 taatatacgc cacctcgcga cagtgag
//
LOCUS       DAPHBJ010000034        30029 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.034, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000034 DAPHBJ010000000
VERSION     DAPHBJ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.034"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            10..1293
                     /gene="yebS"
                     /locus_tag="RHZ76_001418"
     CDS             10..1293
                     /gene="yebS"
                     /locus_tag="RHZ76_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HDV2812080.1"
                     /translation="MALNTPQITPTKKITVRSIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRVRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            1262..3895
                     /gene="yebT"
                     /locus_tag="RHZ76_001419"
     CDS             1262..3895
                     /gene="yebT"
                     /locus_tag="RHZ76_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HDV2812081.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            3975..5414
                     /gene="rsmF"
                     /locus_tag="RHZ76_001420"
     CDS             3975..5414
                     /gene="rsmF"
                     /locus_tag="RHZ76_001420"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HDV2812082.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGVAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCMWLKETYPDAVEFLPLGE
                     LFPAANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            5532..5768
                     /locus_tag="RHZ76_001421"
     CDS             5532..5768
                     /locus_tag="RHZ76_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HDV2812083.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSILYRTRYDQQSDAWIMRLA"
     gene            5873..6064
                     /locus_tag="RHZ76_001422"
     CDS             5873..6064
                     /locus_tag="RHZ76_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HDV2812084.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLHSMSDQRIRQGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(6065..6721)
                     /gene="pphA"
                     /locus_tag="RHZ76_001423"
     CDS             complement(6065..6721)
                     /gene="pphA"
                     /locus_tag="RHZ76_001423"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDV2812085.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERPKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(7117..7458)
                     /locus_tag="RHZ76_001424"
     CDS             complement(7117..7458)
                     /locus_tag="RHZ76_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HDV2812086.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(7471..8343)
                     /gene="copD"
                     /locus_tag="RHZ76_001425"
     CDS             complement(7471..8343)
                     /gene="copD"
                     /locus_tag="RHZ76_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HDV2812087.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(8347..8721)
                     /gene="yobA"
                     /locus_tag="RHZ76_001426"
     CDS             complement(8347..8721)
                     /gene="yobA"
                     /locus_tag="RHZ76_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HDV2812088.1"
                     /translation="MVSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            8860..9090
                     /gene="holE"
                     /locus_tag="RHZ76_001427"
     CDS             8860..9090
                     /gene="holE"
                     /locus_tag="RHZ76_001427"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HDV2812089.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            9192..9848
                     /locus_tag="RHZ76_001428"
     CDS             9192..9848
                     /locus_tag="RHZ76_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDV2812090.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASTRR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            9872..10534
                     /gene="exoX"
                     /locus_tag="RHZ76_001429"
     CDS             9872..10534
                     /gene="exoX"
                     /locus_tag="RHZ76_001429"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HDV2812091.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(10531..12591)
                     /gene="ptrB"
                     /locus_tag="RHZ76_001430"
     CDS             complement(10531..12591)
                     /gene="ptrB"
                     /locus_tag="RHZ76_001430"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HDV2812092.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRNGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPESETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINARPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(12800..13459)
                     /locus_tag="RHZ76_001431"
     CDS             complement(12800..13459)
                     /locus_tag="RHZ76_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDV2812093.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(13786..14142)
                     /locus_tag="RHZ76_001432"
     CDS             complement(13786..14142)
                     /locus_tag="RHZ76_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HDV2812094.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(14209..14499)
                     /gene="yebG"
                     /locus_tag="RHZ76_001433"
     CDS             complement(14209..14499)
                     /gene="yebG"
                     /locus_tag="RHZ76_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HDV2812095.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            14633..15811
                     /gene="purT"
                     /locus_tag="RHZ76_001434"
     CDS             14633..15811
                     /gene="purT"
                     /locus_tag="RHZ76_001434"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HDV2812096.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     SDAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVNAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(15867..16508)
                     /gene="kdgA"
                     /locus_tag="RHZ76_001435"
                     /gene_synonym="eda"
     CDS             complement(15867..16508)
                     /gene="kdgA"
                     /locus_tag="RHZ76_001435"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDV2812097.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(16545..18356)
                     /gene="edd"
                     /locus_tag="RHZ76_001436"
     CDS             complement(16545..18356)
                     /gene="edd"
                     /locus_tag="RHZ76_001436"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HDV2812098.1"
                     /translation="MNPQMLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDGNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(18591..20066)
                     /gene="zwf"
                     /locus_tag="RHZ76_001437"
     CDS             complement(18591..20066)
                     /gene="zwf"
                     /locus_tag="RHZ76_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDV2812099.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            20404..21273
                     /gene="hexR"
                     /locus_tag="RHZ76_001438"
                     /gene_synonym="yebK"
     CDS             20404..21273
                     /gene="hexR"
                     /locus_tag="RHZ76_001438"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HDV2812100.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARKNDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            21401..22843
                     /gene="pyk"
                     /locus_tag="RHZ76_001439"
     CDS             21401..22843
                     /gene="pyk"
                     /locus_tag="RHZ76_001439"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDV2812101.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(22974..23945)
                     /gene="lpxM"
                     /locus_tag="RHZ76_001440"
                     /gene_synonym="msbB"
     CDS             complement(22974..23945)
                     /gene="lpxM"
                     /locus_tag="RHZ76_001440"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HDV2812102.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(24065..25387)
                     /gene="mepM"
                     /locus_tag="RHZ76_001441"
     CDS             complement(24065..25387)
                     /gene="mepM"
                     /locus_tag="RHZ76_001441"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HDV2812103.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(25403..26335)
                     /gene="znuA"
                     /locus_tag="RHZ76_001442"
     CDS             complement(25403..26335)
                     /gene="znuA"
                     /locus_tag="RHZ76_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HDV2812104.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            26414..27169
                     /gene="znuC"
                     /locus_tag="RHZ76_001443"
     CDS             26414..27169
                     /gene="znuC"
                     /locus_tag="RHZ76_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HDV2812105.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVHAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            27166..27951
                     /gene="znuB"
                     /locus_tag="RHZ76_001444"
     CDS             27166..27951
                     /gene="znuB"
                     /locus_tag="RHZ76_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HDV2812106.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAVYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(28292..29302)
                     /gene="ruvB"
                     /locus_tag="RHZ76_001445"
     CDS             complement(28292..29302)
                     /gene="ruvB"
                     /locus_tag="RHZ76_001445"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDV2812107.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(29311..29922)
                     /gene="ruvA"
                     /locus_tag="RHZ76_001446"
     CDS             complement(29311..29922)
                     /gene="ruvA"
                     /locus_tag="RHZ76_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDV2812108.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATNDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         7184 a         7742 c         7579 g         7524 t
ORIGIN      
        1 tggcacgtca tggctcttaa cacaccacaa attacgccga caaaaaagat aacagtgagg
       61 tcaatcggcg aggaactgcc gcgtggtgat taccaacgtt gcccgcaatg tgacatgctg
      121 tttagcctgc ccgagataaa ttctcaccag agtgcctatt gtccgcgctg tcaggcaaaa
      181 attcgcgacg ggcgcgactg gtcgctaacg cgcctggcgg caatggcttt caccatgctg
      241 ttgctgatgc cgtttgcctg gggcgaaccg ctgttgcata tctggctgtt aggcattcgt
      301 attgacgcca acgttatgca aggcatctgg caaatgacca aacagggcga tgcgataacg
      361 gggtcgatgg tctttttctg cgttatcggt gcccccctca ttctggtgac ctccatagct
      421 tatttatggt ttggtaaccg actgggaatg aatctacgcc cggtactgct gatgcttgag
      481 cgacttaaag agtgggtaat gctggatatc tacctggtcg gcattggcgt tgcttctata
      541 aaggtacagg attatgccca tatccaggcg ggtgtgggct tgttctcttt tgtggcgttg
      601 gtgattttaa cgacggtgac gttgtcacat cttaatgtcg aggagctgtg ggagcgattt
      661 tatccgcagc gccccgctac gcgtagggac gagaaactcc gtgtctgtct tgggtgccat
      721 tttaccggct atcctgatca gcgtggtcgc tgcccgcgtt gccatatccc gctacgcgtg
      781 cgtcgccgtc atagtttgca aaaatgctgg gcggcgctgt tagcgtcaat cgttttgttg
      841 ttacctgcca acctgttgcc tatttctatc atttatctga acggaggacg gcaggaagat
      901 acaattcttt ccggaattat gtcgctggca agtagcaaca ttgcggttgc gggaatcgtg
      961 tttatcgcca gtattctggt accgtttact aaagtgatcg tcatgttcac tttactgttg
     1021 agcattcatt ttaaatgcca gcaaggttta cgcacacgca ttctgttact gcggatggtg
     1081 acctggattg gtcgctggtc gatgctcgac ctgtttgtca tatctttaac catgtcgctg
     1141 attaatcgcg atcagatcct cgcttttact atgggaccgg ctgcgtttta tttcggcgca
     1201 gcggtaattt tgactattct tgctgtggaa tggctggaca gccgcttact ttgggatgca
     1261 catgagtcag gaaacgcccg cttcgacgac tgaagcgcag attaaaaata aacgccgtat
     1321 ctcacctttc tggctgctgc ctttcatcgc gctaatgatt gccggttggc tgatttggga
     1381 cagttatcag gaccggggta ataccgtcac catcgacttt atgtcggcgg atggtattgt
     1441 tccgggccgt acgcctgttc gttatcaggg cgttgaagtc ggaacagtgc aggatatcag
     1501 cctcagcgac gatcttcgta agattgaagt caaggtcagc atcaagtccg atatgaaaga
     1561 tgcgctgcgc gaagagactc agttctggct ggtgacgcca aaagcatcgt tggcaggtgt
     1621 ctccgggctg gacgccctcg tcggtggtaa ctatatcggc atgatgccgg gtaaaggtaa
     1681 agagcaggat cactttgtcg cactcgatac ccaaccgaaa tatcggctgg acaatggcga
     1741 tctgatgatc cacctgcaag cccccgatct cggttcgctg aacagcggtt cattggtcta
     1801 tttccgcaag atcccggtgg gaaaagtcta cgactatgcc atcaatccca acaagcaagg
     1861 cgtggtgatt gatgtcctga tcgagcggcg ttttaccgac ctggtgaaaa aaggtagccg
     1921 tttctggaac gtttccggcg ttgatgccaa cgtcagtatc agtggcgcga aggtgaaact
     1981 ggaaagtctg gcggcactgg ttaacggtgc gattgccttc gattcaccag aagagtcgaa
     2041 acctgccgag gcggaagata cctttggtct gtatgaagat ctggcccaca gccagcgtgg
     2101 cgtaataata aaactggaac tgccgagtgg ggcaggatta accgccgact cgacgccgtt
     2161 aatgtatcag gggctggaag tcggacagct gactaaactg gatttaaatc ctggtggtaa
     2221 agtcaccgga gaaatgaccg ttgatcccag cgtcgttacg ctgttacggg aaaatacccg
     2281 catcgaatta cgcaacccga aattatccct tagcgatgcc aatctcagcg ccctgctgac
     2341 tggcaaaacc ttcgagttgg tacccggcga tggcgagcca cgcaaagagt tcgttgttgt
     2401 gccaggcgaa aaagcactgc tgcatgaacc tgatgttctg acgctgaccc tgaccgcacc
     2461 ggaaagttac ggtattgatg cgggtcagcc gctcattctt cacggcgtgc aggtaggcca
     2521 ggttatcgat cgtaaactca ccagcaaagg cgtcaccttt accgtcgcca tcgagcctca
     2581 gcatcgagaa ctggtaaaag gcgatagcaa atttgtcgtc aacagccgtg tcgacgtgaa
     2641 ggtggggctg gatggcgttg agtttctcgg tgccagcgcc tcagaatgga ttaacggcgg
     2701 gatacgtatt ctgccgggcg ataaaggcga gatgaaagcc agctatccac tgtatgccaa
     2761 tctggaaaaa gcgctggaga acagccttag cgatttaccc accacaaccg tgagtttgag
     2821 tgcagagacg ctgccggatg tgcaggcagg atcggtagtg ctctaccgta aatttgaagt
     2881 tggtgaagtt attaccgtcc gtccgcgagc taacgcgttt gatatcgatc tgcatattaa
     2941 gccggagtat cgcaaccttc tgaccagcaa tagcgtgttc tgggcagaag gcggggcgaa
     3001 agttcagctg aatggtagtg gcctgaccgt acaggcatcc ccgctctcca gagcattaaa
     3061 gggagccatt agcttcgata acctcagcgg tgccagcgcc agtcagcgta aaggcgacaa
     3121 acgaattctg tatgcttccg aaacagcggc ccgtgcggtt ggtgggcaga ttacgcttca
     3181 cgctttcgat gccggaaaac tggcggtcgg gatgccaatt cgctatctcg gtattgatat
     3241 cgggcaaatc cagacgctgg atctgattac cgcgcgcaat gaagtacagg caaaggcggt
     3301 gctctatccg gaatatgtcc agacctttgc tcgcggtggt acgcgcttct cagtggtcac
     3361 accgcaaatt tcggcagctg gcgttgagca tcttgatact atcctccagc cgtatatcaa
     3421 cgtcgaacca ggccggggca atcctcgccg cgactttgaa ttacaagagg ccaccattac
     3481 tgattcgcgt tacctggatg gcttaagcat tattgttgaa gcgccggaag ccggttcgtt
     3541 aggcatcggt acgcctgtgc tgttccgtgg tctggaagtc ggtacggtta caggaatgac
     3601 gctggggaca ttgtcagatc gcgtgatgat tgcgatgcgc atcagtaaac gctatcaaca
     3661 cctggtgcgt aacaattccg tcttctggtt ggcatcgggt tacagtctgg actttggtct
     3721 gacgggcggc gtagtgaaaa ccggcacctt taaccagttt atccgtggcg gcatcgcctt
     3781 cgccacgcct ccggggacgc cactggcacc gaaagcccag gaaggcaagc acttcctgtt
     3841 gcaggaaagt gaaccgaaag agtggcgtga atggggtact gcgcttccca aataatgccc
     3901 actgctccgg cgtgcctgcg ccggagcgtt tatgctaaac tgcgcgcctg tttttttgcc
     3961 agtggtatat gctcgtggcc caacacaccg tttatttccc ggacgccttt ctgacgcaaa
     4021 tgcgcgaagc gatgccttcg acgctctctt ttgatgattt tcttgccgcc tgtcagcgcc
     4081 cgttgcgccg cagcattcgc gttaatacgc tgaaaatctc cgttgctgat ttcctgcaat
     4141 taaccgctcc ttatggctgg acgcttacgc caattccgtg gtgtgaagaa ggtttctgga
     4201 ttgaacgcga caatgaagat gcattgccat tgggtagtac cgccgagcat ttaagcggcc
     4261 tgttttatat tcaggaagcc agttcaatgt tgcccgtcgc cgccttgttt gctgacggta
     4321 atgcaccaca gcgggtgatg gatgtcgctg ctgcgccagg ctccaaaacg acgcaaattg
     4381 ccgcacggat gaataacgaa ggggcaatcc ttgccaatga gttttccgcc agtcgggtaa
     4441 aagtgttaca tgccaatatc agccgctgtg gcatcagtaa tgttgcgctc acacattttg
     4501 atggccgcgt gtttggtgtg gcagtgccag aaatgttcga tgccattttg ctggacgctc
     4561 cctgctctgg cgaaggcgtg gtgcgtaaag atcccgatgc gctaaaaaac tggtcaccag
     4621 aaagcaatca ggaaatcgca gctacacaac gggagcttat cgacagcgcc tttcatgcat
     4681 tacgtcctgg tggtacgctg gtttactcga cctgtacctt aaacagggaa gaaaacgaag
     4741 ccgtttgcat gtggctgaaa gagacttacc ctgacgcagt agagttttta ccacttggcg
     4801 agctcttccc tgctgcaaac aaagcgctga ccgaagaagg ctttttgcat gttttcccac
     4861 aaatttacga ctgcgaaggc ttcttcgttg ctcgtctgcg taaaactcag gccattcccg
     4921 ccttacccgc ccccaaatac aaagtcggta attttccgtt cagcccggtg aaagatcgcg
     4981 aagctggaca aattcgtcag gcggctgcag gtgttggctt aaactgggat gaaaacctgc
     5041 gcctctggca gcgtgacaaa gaactgtggt tgttcccggt gggcattgaa gccctgatcg
     5101 gtaaagtccg attttctcgc ttggggatta aacttgccga aacgcacaac aaaggttatc
     5161 gctggcagca tgaagcagtt attgcccttg ccacccccga caatgtgaac gcttttgaac
     5221 tgacaccgca ggaagcggag gagtggtatc gcgggcgcga tgtttacccg caagccgcgc
     5281 cagtggcgga tgacgtgttg gttactttcc agcatcagcc gattggttta gccaaacgga
     5341 ttggttcgcg actgaaaaac agctacccgc gtgaactggt gcgggacggg aaacttttta
     5401 ccagtaacgc atgacagcgc acaaaaaaag cgcacttttt gactggcaca ttcggcggcc
     5461 tcaactaggc tgaaaaatgg tgcgatcgga ctggtcgtac cacaatcggc agctaaatgg
     5521 agagcacgaa gatgaaaacc agtgtgcgca taggcgcttt tgaaatcgac gacggcgaat
     5581 tacacggtga atcgccgggt gatcgaacgt taaccattcc ttgtaaatct gaccccgatt
     5641 tatgtatgca actggatgcc tgggatgctg aaaccagtat ccctgccctg ctaaatggcg
     5701 aacactctat cctttaccgt acccgttacg atcaacaatc tgatgcttgg attatgcgtc
     5761 ttgcctgatc caaaaagaac ccgtcggcat ggcgggttat ttgtcctggt tattcccccg
     5821 ttgtaaaatc tctcctaaac ttaacggtac ggcaccacac ttcggggatg aaatgttcgc
     5881 gctggtactt tttgtttgct acctggatgg cggttgtgaa gatattgttg tggatgtcta
     5941 caacacggaa cagcagtgtc ttcattctat gagcgatcaa cggatccgcc agggcggttg
     6001 ttttccgatt gaggatttta tagatggttt ctggcgacct gcgcaggagt acggtgattt
     6061 ttaattattg caattgcaca agagtcagtt cgcccccaaa gacagcaccg gtatcaatat
     6121 aatgcaggtt gccaatatcc acgcgatgtc gcaacggtgt atgaccaaac cagaaatgat
     6181 cagcacctgt aattccctgc ccttttgggc gttcacctaa tcgtgagcgg ctccacaaga
     6241 cctgatgcaa atcaacgtcc ttttgccatt cataaacatc atctggataa tcggcatgag
     6301 caataacatg tttgccggtg cggctgtgta cttcaagaat aaagggcaaa tgctgacatt
     6361 tttccagcgc cgttttcgct tgtttctgtt gattatctgc cagcgcaata aaccagtcgc
     6421 cgccattcat caaccacaaa gacatctgct gggatgccag cgcatccatc gccatctgtt
     6481 catgattgcc tcttaccgca cgaacccaat gttgttccag taactgcaga caacgtaaac
     6541 tttgcggccc acgatcgata acgtctccca ctgagataag taaatctcgc cacggatcaa
     6601 aacgacaatg ccataatttg cggcgcaact gctcaagaca accgtgtata tcgccagaaa
     6661 gccagatatg tcgccattga tgacccgcaa ttctctgata aacgggcgca ggctgtttca
     6721 tcaatatttt cctcccgcgc taaagatcac ataatcttaa caagaatgtt aaaaaacgct
     6781 ggactcagac agtagagtgt gtgttatggt tgactataaa gtcagcgaag gaaatgcttc
     6841 tggcttttaa cagataaaaa gagaccgaac acgattcctg tattcggtcc agggaaatgg
     6901 ctcttgggag agagccgtgc gctaaaagtt ggcattaatg caggcttagt tgccttgccc
     6961 tttaagaata gatgacgacg ccaggttttc cagtttgcgt gcaaaatggt caataaaaag
     7021 cgcggtggtc atcagcttaa atgttaaaaa ccgcccgttc tggtgaaaga actgaggcgg
     7081 tttttttatt ggaaatcaaa aggctatttt aggtaattaa cagagttttt cagctcgttc
     7141 tataaacggt gccagactca ttttttcgcc gggattgtta ggatcatcaa tctgaatcac
     7201 cgaaatgggt tgggctttag tcttcccact ggcaacttcc ttttgtgcga tatcgtttaa
     7261 aggatactgc acgagggtac ttgggttgat gacatacaaa gcattacccg gtcggcaagt
     7321 cagcatcacc tcttcgcgat taaacgccca tttgtcttta cccacttcaa aacggctgac
     7381 ggtaatcacc tgcggtgccg ccagcgccgc tgcagaactg gtgagtaaca gaaacgccag
     7441 aatacttttt ttcatcattt tcgtaatcca tcaaaaaggt tcgagagtcg caagcaggct
     7501 gatgatcacc agcactaccg cacctatcgc ccattcgagt ttagtcatcc acacaaacca
     7561 gggtgccgca cgatcttcat cctgcctcat gcgcggtaca agaacataac gattagccag
     7621 cgcgatgacc accataatca tcaccaggat agctttgagc aataagagtt ggccccagta
     7681 agtcgtaagt gtggggggaa atccggtaat caacaatgca ttaagcacgc cgcttgccag
     7741 tacgccgatc accgcaaaat gcccgcacca ggaaaaacgc atcagcgcct gaatagcctg
     7801 gtgtcgccaa cggcctttga tgagttgcat acaccagagc accggcagta atcccccaaa
     7861 ccacgccgcc gcacaaatca ggtgaatcgc gtgattagtc tgatggattt tcgccgttac
     7921 cccttcattc agcgtcgcat gccccactcc cgccagcaga ataaattgcg cggtagtgag
     7981 cataaacagc aaacgtggca tattccgcgg ttgcataagg gcgacgatca acgttacgag
     8041 ggcgagaaca atttgccata accagacgcc accaaactgc gtttgcaaaa cagcctgcca
     8101 aatgtttggc gaaaatacat ctgtccatcc cgttcccatc aacccgccct gaacggcgag
     8161 cattgcagtg gcactgatca aactccagac ggcagcgtgc tgttgtaaac gcagaaaacg
     8221 cttcgccagc aagcgacgaa tcgttaaggg ggccagccag gcaccgtaca ttgcgaagcc
     8281 aaaaaccagc atcagcgagg tgaaatggat aaatcgcagc gcgatccagg taaacgccag
     8341 catgatttat ttcacgctaa aggtgtagtg tcctttcgtt ttatgcccgt ccaccgaaac
     8401 aacatgccag tcaacggtgt aggttccggg tttcagcgaa tcggccagtg gaacaatcag
     8461 ttgcttttgg tcctgctcat ttcgcttcgc cggtaatgtt ttaatatttt cgttttttgg
     8521 ccccgtgatt tttgcaccgc tgaatcctgt ttcaacacct tccgagaagt ttaaagtgat
     8581 tgcctgcggg gcagctgtca cttgcgcgtt tgccgcagga tactgatgcg ttaaatgcgc
     8641 atgtgcccag acggaaggtg tcaccaacga agtggtcagg attgccagcg cgtagcgaag
     8701 ggagcgtgca gttgaaacca tattatctat tcctttttgt aataactttt ttacagagca
     8761 taaccttgtc taatgtctga gtcgaggatc atcaattccg gcttgccatc caggctcact
     8821 cttagtaact tttgcccgcg aatgatgagg agattaagaa tgctgaagaa tctggctaaa
     8881 ctggatcaaa cagaaatgga taaagtgaat gtcgatttgg cggcggccgg ggtggcattt
     8941 aaagaacgct acaatatgcc ggtgatcgct gaagcggttg aacgtgaaca gcctgaacat
     9001 ttgcgcagct ggtttcgcga gcggcttatt gcccaccgtt tggcttcggt caatctgtca
     9061 cgtttacctt acgagcccaa acttaaataa aacttataca gagttacact ttcttacata
     9121 acgcctgcta aattatgagt attttctaaa ccgcacccat aatttgtagt cattttgaaa
     9181 aggaagtcat tatgtcgttc tggaaagttg cagcagcgca atatgagcct cgtaaaacct
     9241 cactcaccga acaggtagct catcatctgg agtttgtgag ggccgccgcc aggcaacaat
     9301 gtcagctgtt agtttttcca tcgctttctt tactggggtg tgattattcg cgacgtgccc
     9361 ttcctgcccc acccgatctc tcactgttag acccgctttg ttatgccgca acgacctggc
     9421 gaatgaccat catagccggc cttcccgttg aatataacga tcgctttatt cgtggaattg
     9481 cggtgttcgc accgtggcga aaaacgccgg ggatctacca tcagagtcat ggtgcatgtc
     9541 tgggccgtcg ttccagaacc atcacggtgg tagatgaaca accgcaaggt atggatatgg
     9601 acccgacctg ttcactgttt acgacagggc aatgtctggg ggaacccgac ctactggcat
     9661 ctacccgccg cttacaattt ttttcacatc agtactccat tgccgtgctg atggcaaatg
     9721 cccgtggtaa cagtgcacta tgggatgaac atggtcgtct catcgttcgc gccgatcgtg
     9781 gttcattatt gttagtcggt cagcgttcat cacagggttg gcaaggcgat atcattccat
     9841 tacgctaggc tttttcggcc tggagcatgc catgttgcgc attatcgata cagaaacctg
     9901 cggtttgcag ggagggatcg ttgagattgc ctctgttgat gtcattgacg gaaaaatcgt
     9961 caaccccatg agccacctgg tgcgccccga tcgtcctatt agtccacaag cgatggcgat
    10021 tcatcgcatc accgaagcca tggtcgccga taaaccgtgg attgaagatg tgatcccaca
    10081 ctattacggt agtgaatggt atgtcgcgca taacgccagc tttgaccgcc gcgtactgcc
    10141 tgagatgccc ggtgagtgga tttgcactat gaaactggcc cgtcgtttgt ggccagggat
    10201 caagtacagc aatatggcgt tatataaaac acgcaagctc aatgtacaga cgccgccggg
    10261 cctgcatcat caccgcgcgt tgtatgactg ttatatcacc gccgcgttgc ttatcgatat
    10321 tatgaacacc tccggctgga cggcagaaca gatggccgat atcaccggac gtccgtcgtt
    10381 gatgacgacc ttcacctttg gcaaataccg tggcaaagcg gtttccgacg ttgccgaacg
    10441 cgatccgggc tatctgcgct ggttatttaa taacctggac agcatgagcc cggagctgcg
    10501 tttaacactg aaacattatc tggaaaatac ttaatccgca ggcgtagcgg gtaatgttcc
    10561 ctgcgccagc gcgaccagaa aagcatattc catcgctacg ccttcgtacg atttaaagcg
    10621 accagattta ccgccatggc ctgagtccat gtcggtacag agcaataaaa gatggtcatc
    10681 ggttttcagc tcgcgcaatt tagcgaccca ttttgccggt tcccaatatt gcacctgaga
    10741 atcgtgcaaa ccggtcgtta ccagtaaatg cggataagcc tgtgcggtga cgttgtcata
    10801 tgggctgtag cttttcatat actcgtaata ttgcggatcc tgcggattcc cccactcttc
    10861 aaactcacca gtggtaagag gaattgattc atcaagcatt gttgttacaa catcaacaaa
    10921 cggtacctgg gcgataacgc cgtggaataa ttcagggcgt gcattaatcg caacgcccat
    10981 caacatgccc cccgcactcc cgcccatcgc ataacaaagc gaaggagagc catagcccag
    11041 ttttaacaat gcatcgcagg catcaagata atcattaaac gtatttttct tcttcagaaa
    11101 ttttccgtct tcgtaccatt gttgccccag ctcaccaccg ccgcgaacat ggacaatggc
    11161 gtagacaaag ccacgatcta acaaactcaa gcggctaaaa ctgaaatcgg catcaatact
    11221 tgcgccgtaa gaaccatagc catacaccag cagcgggttg tgtcctttgc gaaaatgttt
    11281 gcgatgatag accagcgaaa ccggaacttc gacgccatca cgggcgacta tccacaggtg
    11341 ttcactgcgg taattcgccg catcaaaacc aggaacttcc gtttgtttta atacacgacg
    11401 ctcaccggta tccatatcca gttcaaacaa agtgtctggt gtggtcatgg aagaataacc
    11461 ataacgcaat cgcgcggttt cagattctgg attgtaggca atccaggtca cataggccgg
    11521 atcatcaaag gcaataccaa tgacttcccg ggtcttgcgg ttaatttggc gcaaactggt
    11581 taacccgcgc tgacgctctt caaccaccag ccagtcggta aacagcgtaa acccttccag
    11641 catgatgttt tcgcgtggcg gaattaactc ttcccactgt tgctcatcac gcatacgggt
    11701 acggtataag ccaaagtttt tgccgttgcg gttggaacgc agataaaacc gatgctggta
    11761 gtgatcaagg ctgtattcgt gatctttgcg gcgcggcaga aaaacaaacg gctcggcatc
    11821 ggccatttcc gcgtccagca ggcgaacttc actggtggtg gcgctggcca aatgaatgac
    11881 tacatagtgc ttcgacgtcg ttttatgcag gctgacgtaa taggtatcgt ctttttcttc
    11941 gtagatcagt ttatcttgcg atgctggagt accgatagcg tgacgccaga cctgataagg
    12001 cagcagcgtc actggatgct tgcgaacata gtagaaagtc caggagtcat ttgcccagac
    12061 aaagctgggt tcaacgttat ccagcagttc cgggtaccag ttacctgttt ccagattacg
    12121 aaaacgaatg ccgtactggc gtcgggaaag aaaatcttct gccagcgcca taatggtgtt
    12181 atcgggcgta atcgccattc cgcccatcga ataaaactca ctatgagccg cgcgcttgtt
    12241 ggcatcgagc aatatttccc actcgtccca ctcttcactg aacgccgatt gacgctggta
    12301 gatagcatat tcacagcctg gttcataaat atgccgatag cggtagccat ttttgatgta
    12361 gggcgcagaa acttctcttt gcggaatgcg gtcgatgatt tcctttaaga tgcgatcctg
    12421 caaggcttgt tgtgaggcca tcacccgatg accgtaacta ttttcttgtt gcaggtagtc
    12481 caggacttcc ggctgagaac gcgtatcgtc ccgcagccag tagtaattat cgatgcgcgt
    12541 atcgccatga agcgtcatgg cgtggggaat gcgggcggct tttggtagca tgttattgtt
    12601 ctttctggtt gaaacatctt ataagggtgg caaaactcac cggggatgca agcgaaacag
    12661 ggcagtcatt gcttagatga tgacaggtaa tggcgcgaat atcgaatgtt atgcagacag
    12721 agaaaatcag cctgttcaaa tggctgtgcg gttctggata gcccgaaata gtcaacttca
    12781 ggctatccag agagcgggat tattccgcca aagtgcgttt ttgctgttcg agatcgcgtt
    12841 caatgccttc acgaacatcc tgggggattt tcagcgcgtc acccagtgca ttcaggtaac
    12901 tgcgttccat aaaatggtca atatcaatag ccgcgcaact caggaaatag atttccagcg
    12961 cctcttcttc attgcggacc ccggtagcca ggcgttgtgg atccagcggt tgttcgattg
    13021 cctgctcaat gagtacacgc ccctgctctt ccacgccggc ttcacgcaat tgctggtcga
    13081 ttgccgcacg ttctttggca tcaatatgac catcactttt agcggcaaag accagcgcaa
    13141 ggatcaaacg ttctgtacgc tcatccagcg gcgtactttg cgcgccaaac tgcggttcgt
    13201 cctgatgcgc cgcgcgaatt ttatctttgt atttattcca cagcaccgta cccgctaccg
    13261 ctccgccgcc aaccagtaac gcgttggtgc catatttcgt aagaagttta cgtgctgatt
    13321 tatttgcgac cagcagccca gccagtccgc ctaatgcgcc tggcactaac agtttgccca
    13381 atccctgatc cgcagacgag gaggtagaag aactgctttg cccaagcagg gattgcagtt
    13441 gatttaacca gttagccata tttgctcctc aataaccatt tttagtgcct gtagcatagc
    13501 ggatgaggat gtcagaaaat gtatatcgag acaaaagatg cgcaaataag cggggcggta
    13561 ggccgttatt caaagaaatt cgcgccaggt aaggtattta ctgtatatca ggtgataagg
    13621 gtattttagg tgaaagtagt ttgtcgtttt gagtggcatt gtttgatgct gattgcccga
    13681 tgcgacgctg ttgcgtcttc tcaggcctac gattgtggtg cgttctgggg cggacaaggc
    13741 actcgcgccg catccgccaa cggtcaatcg aattatcaga agccattaac gccgctgata
    13801 ttccgccatt cccgctttgc agtccacgct gacctggtaa tgaatatcgg cacttttacc
    13861 acgcacggtt agcggtactg accatttatc atctttaccc tgaatgtcct gcaaactgac
    13921 ccaagccacg ggatcggcct gaccgacaat tttttgatca tctgcccaac gcgccacgcg
    13981 attttgttga taatcacgtt ttacgctcgc ggcaattccg gcagcatcca gaccttcaca
    14041 ctttgggaaa gtgaccgact tgctggtttc attattggca gcgaaaactg atgcgcaggc
    14101 agaaaccaac aacagcccta aaaacgcccc tctttttttc atgtttttct ccatagcaca
    14161 atgattcagg agaaagcatg gtacaaattg tcaggagcgc aagttgcttc aggccgcgtg
    14221 tgaggcatct tcctcttcgg attcagcacc gacgacctgt ggggacggta atttgccggt
    14281 tttcagaatg gtgctcagca catctttttg ttctgccagc catagcgaaa gggcttcacg
    14341 ttgctcgtct tccatttgca ctggagaatt tgtcagccag gtgtcaagca gatccgccgt
    14401 atcaagcatt ttgtcataag catcggcttc ctttttgctg gtaaacgaca ttttctcttc
    14461 gccctcacga atgactacgt atttaacttc aaccgccatt tgcagcctct cataataact
    14521 gtgattttat acagtatatt tcttttcggt tgagaaatca acatcagcaa taaagacaca
    14581 cgcaaacgtt ttcgtttata ctgcgcgcgg aattaatcag gggatattcg ttatgacgtt
    14641 attaggcact gcgctgcgtc cggcagcaac tcgcgtgatg ttattaggct ccggtgaact
    14701 gggtaaagaa gtggcaatcg agtgtcagcg tctcggcgta gaggtgattg ccgtcgatcg
    14761 ctattccgac gcaccagcca tgcatgtcgc gcatcgctcc catgtcatta atatgcttga
    14821 tggtgatgca ttacgccgtg tggttgaact ggaaaaacca cattatatcg tgccggagat
    14881 cgaagctatt gccaccgata tgctgatcca acttgaagag gaaggactga atgttgtccc
    14941 ctgcgctcgc gcaacgaaat taacgatgaa tcgcgagggt atccgtcgcc tggcggcaga
    15001 agagctgcag ctgcccactt ccacttatcg ttttgccgat agcgaaagcc ttttccgcga
    15061 ggcggttgcc gccattggct atccctgcat tgtaaaaccg gtgatgagct cttccggcaa
    15121 ggggcagacg tttattcgct ctgcagagca acttgctcag gcatgggaat acgctcagca
    15181 aggcggtcgc gccggagcgg gccgcgtaat tgttgaaggc gtcgttaagt ttgacttcga
    15241 aattaccctg ctaaccgtca acgcggtgga tggcgtccat ttctgtgcac cagtaggtca
    15301 tcgccaggaa gatggcgact accgtgaatc ctggcaacca cagcaaatga gcccgcttgc
    15361 ccttgaacgt gcgcaggaga ttgcccgtaa agtggtgctg gcactgggcg gttatgggtt
    15421 gtttggtgtc gagctatttg tctgtggtga tgaggtgatt ttcagtgagg tctcccctcg
    15481 tccacatgat accgggatgg tgacgttaat ttctcaagat ctctcagagt ttgccctgca
    15541 tgtacgtgcc ttcctcggac ttccggttgg cgggatccgt cagtatggtc ctgcagcttc
    15601 tgccgttatt ctgccacaac tgaccagtca gaatgtcacg tttgataatg tgcagaatgc
    15661 cgtaggcgca gatttgcaga ttcgtttatt tggtaagccg gaaattgatg gcagccgtcg
    15721 tctgggtgtt gcgctggcga ccgctgagag tgttgttgac gccattgaac gcgcgaagca
    15781 cgccgccgga caggtaaaag tacagggtta aacccggcaa aaaaacgcta caaaaatgcc
    15841 cgatcctcga tcgggcattt tgacttttac agcttagcgc cttctacagc ttcacgcgcc
    15901 agcttagtaa tgcggtcgta atcgcccgct tccagcgcat ctgccggaac cagccaggaa
    15961 ccaccgatgc acagcacgct tttcagcgcc aggtagtcac ggtagttagc cggagaaata
    16021 ccacccgtcg ggcagaaacg gacctgggag aacggacccg cgatcgcctg cagggctttc
    16081 acgccgccgt tagcttcagc cgggaagaat ttgaactctt tcaaaccgta gtccataccc
    16141 agcatcagtt cggaaacagt gctgatcccc ggaatcagag gaatagtccc ttcggtagca
    16201 gctttcagca gcggctcggt cagacccggg ctaattgcga actgtgcacc cgcttcagtg
    16261 acttctgcca gctgctgtgg attcagcacc gtaccggcac ccacaatcgc ttcaggcact
    16321 tctttggcga tagcacggat agcgtcaact gcacactcgg tacgcagagt cacttccaga
    16381 acgcgcaccc caccagcaac caacgctttt gccatcggca ccgcgtgttc cagttttttt
    16441 accacgataa ccggtacaac cgggccggtg gtcaggattg attctgcact tgttttccag
    16501 tttttcatca gagttttctc tcgcctgatt acaaatttgt cgtcttaaaa agtgatacag
    16561 gttgcgccct gttcggcacc ggacagtttt tcacgcaagg cgctgaataa ttcacgtcct
    16621 gttcccacgc gtgacgcgct caggtcagga atgtgcggtt cgcgagcagc cagttccgct
    16681 tcgtctacca gcagcgtcag ttcgcctgtc tgtccattca cacgaatgat gtccccgtcg
    16741 cgcacttttg ccagcagccc gccatcgtag gcttctggtg ttacgtggat agctgacggc
    16801 actttacctg aagcgccgga gagtcgtcca tcggtaacta acgcaatttt gaaacaccgg
    16861 tccaataata caccaagtgg cggcatgagt ttatgtaatt ctggcattcc gttcgctttt
    16921 ggcccctgat gacggacaac aacgacacaa tcgcggtcca gcaaacccgc ttcaaaggcc
    16981 ggcataacgt catgctggct ttcaaaaaca accgctggcg cttcaatcac ctggttctca
    17041 accggcacgg cagaggtttt cataaccgca cggcccaggt taccgcttaa cacttttgtc
    17101 ccaccatgat gagagaaagg ttgttcgaag gaagcgatca cattgccgtc gagtgatttt
    17161 tccgcccctt cccgccagtc cagttcacca ttattcagcc atggttcaag cgtataacga
    17221 gacagaccaa agcctgccac cgtattgaca tcttcatgca gcagacctgc tttgagcagt
    17281 tcacgcacca gaaccggtac gccacctgcc gcctggaagt ggttaatatc ggccggaccg
    17341 ttcgggtaga gacgtgccat cagcggtaca acatcagaaa ggtcagagaa gtcatcccag
    17401 ttaatctgaa taccggccgc gcgcgccatc gccaccaggt gcatggtgtg gttagtggaa
    17461 ccaccggtcg ccagcagtgc aacaataccg ttcaccacca ctttctcatc gatcatctta
    17521 ccgatcggca tccattcatt accattaccg gtcatgcgtg taacctgacg cgcagcggca
    17581 gcggtcaaag catcgcgcag cggagagtcc ggatgaacaa aagaagagcc tggcaactgc
    17641 atccccataa actccaccac catctggttg gtgttggcag taccgtagaa agtacatgtt
    17701 cccggcgcat gataagacgc ggcttctgac tccagtaggg ccatgcggtc cactttacct
    17761 tcggcataaa gctggcgaat acgcactttt tctttatttg gcaaaccgct tgccatcggt
    17821 ccagacggta caaacaccgc aggcaaatga ccaaacgaca gggctgccat cgtcagaccc
    17881 gggacaatct tgtcgcacac cccgaggaac agagcaccat caaacatgtt atgggacagc
    17941 ccgaccgccg cagacatcgc tatcacttcg cggcttagca gcgacaattc cattccatcc
    18001 tgcccctggg tgacaccatc acacatcgcc ggaacaccgc ccgcaacctg accaaccgca
    18061 ttcgcttcat gcagggcttt acgaatgatt tctggatagt gttcataagg ctggtgcgcg
    18121 gagagcatgt cgttatagga ggtgatgatg gcgatattgt tacgcaacat gcttttcaaa
    18181 gaggctttgt cttctggctg gcaggcagcg aaaccgtgtg ccaggttacc gcatgccaac
    18241 tgcgaacgat gaacggtcga cgttttcgct tgttctatcc gggcgagata agcagagcga
    18301 gtctcgcgcg aacgttcaat gattcggttt gttacgcgta acatttgtgg attcataaag
    18361 gctcctgaaa ttgagttgtc agagcaggat gattcacaac gcgtttcatt cagaggattt
    18421 atgagtgaaa cgcctgtaac cggagctcat agggcaaaaa cgtttcagtc agtgtaataa
    18481 aaaaagcctc gtgggtgaat ccgcacgagg cctgaaagtg taaaaattgt tctacaatct
    18541 gcgcaagatc atgttaccgg taaaataacc ataaaggata agcgcagata ttactcaaac
    18601 tcattccagg aacgaccatc acgggtaatc atcgccaccg aggcaacggg tccccaggtt
    18661 ccggcctgat acggtttcgg cgcatcattg tccatcgccc acgcctcagt aatggagtct
    18721 acccatttcc aggcttcttc cacttcatca cgacgtacaa acagtgcctg aataccacgc
    18781 atggtttcca gcagcagacg ttcataggca tccgccagat gcgtctgatt aaaggtttct
    18841 gaatagctca gatccagctt ggtgatttgc aggttatgtt tgtggtcaag gccaggaact
    18901 ttattcagta cctggatatc cacgccttca tcaggttgca gacggatagt cagtttattc
    18961 tgcggcagat cctgccacga ctctttaaac agattcagtt caggtgtttt gaaatagacc
    19021 acgacttcag aacatttggt cggcagacgt ttaccagtac gcaggtagaa tggcacaccg
    19081 gcccagcgcc agttatcaat gtcgacgcgg atcgccacga aggtttctgt attgctgctc
    19141 ttgttcgcgc cctcttcttc cagatatccc ggcacttttt tgccctgggc gaagcccgca
    19201 gtatattgcc cacgtacggt tttttcgcgt acgttggagc ggtcgatgcg gcgcagggac
    19261 ttcagtactt tcactttttc atcgcggatg ctgtctgcgc tcaggtcaga tggcggagac
    19321 atcgcaatca tgcagagaat ttgcagcagg tggttttgga tcatatcgcg catctgaccg
    19381 gctttatcaa aatagcccca gcgcccttcg atccccactt cttctgccac ggtaatctca
    19441 acatgatcaa tggtgcgatt gtcccagtta ttcacaaaca gggagttagc aaaacgcagc
    19501 gccaacaggt tcagcaccgt ttctttacca agatagtggt cgatacggta aacctggcac
    19561 tcctcgaagt attcgccaac ctgatcattg atttcctgcg aggtcgccag cgacgtcccc
    19621 agcggtttct ccatgactac gcgtgccggt ttagcattca gtttagcctc gccaagccct
    19681 ttgcaaattg cgccaaaagt gctgggcggc atggcaaagt agttaatggt gatacgattt
    19741 ttttgatcca gcatcgcgcc gagacggctg aatgcagcag tgtcattgac atcgagatta
    19801 caaaaatcca gacgtgcact cagggtgtcc cataaacctt catcaatggt ttctttcatg
    19861 aaagtttcga gcgcctcgcg gacaactttg gtatatgccg ctttatccca gtcagcacgc
    19921 cctacgccga taatccgggt gtccgggttg agctgaccgg ctttttccag ttgatacagg
    19981 gaaggcagca atttacgacg cgcaaggtcg cctttcgcgc cgaaaatgac caggtcacag
    20041 gcctgggctg tttgcgttac cgccatgtca ttctccttaa gttaactaac ccggtactta
    20101 agccagggta tacttgtaat tttcttacgg tgcactgtac tgcttttacg agcttgcgaa
    20161 aactgtaaac gcttatccac ccgtgcgatt acgggaaaag cgcgcaaagt gcggcaaaac
    20221 tgataaaaaa atcatcgttt tccattgggt aaaaatctga cactgatcat gttatgaaaa
    20281 aaaataacaa cttttttatc tgcttttgtc attaacacgg cacacaggcg taatatctga
    20341 caaaactgca tttcgatttc tttcagtgcg gaaatcgtca ttacccgtga gtctctttac
    20401 atcatgaata tgctggaaaa aatccagtct cagctggaac atttgagcaa atcagagcgc
    20461 aaagttgccg aggtcattct ggcttcgccc gataacgcga tccattcgag tattgctgct
    20521 atggcactgg aagccaatgt tagcgaaccg acggtgaatc gtttctgtcg cagcatggac
    20581 acgcgcggtt ttcctgattt taaacttcat ctggcacaga gtctggcgaa tggcactccc
    20641 tatgttaatc gcaatgtcaa tgaagatgac agcgttgaat catacacagg gaaaatattt
    20701 gagtccgcaa tggcaacgct tgatcatgtc cgtcattcac tggataaatc tgccatcaac
    20761 cgcgccgtcg acttgctcac tcaggcaaaa aaaatcgcct ttttcggatt aggctcttca
    20821 gccgccgttg cccacgatgc gatgaataag ttctttcgtt ttaatgttcc ggtggtgtac
    20881 tccgatgata tcgtgctgca acgcatgagt tgtatgaatt gtagcgacgg agacgtggtg
    20941 gtgctgattt ctcacactgg aagaacaaaa aatctggtcg agctggcgca gctggcacgc
    21001 aaaaacgacg ccatggtgat tgccctcacc tctgcgggta ccccgctcgc ccgggaagca
    21061 acgctggcaa ttaccctcga cgtaccggaa gatactgaca tttatatgcc catggtttct
    21121 cgacttgcac agctgaccgt gatagatgtg ctggcgacag gatttacttt gcgacgcggt
    21181 gcaaaattca gagataactt gaagcgggtc aaagaagcgc tgaaggaatc gcgttttgat
    21241 aagcagttac ttaatttaag tgacgatcgc taaaaacgac tgtcactgtc ctaatcttat
    21301 acgacatccg aatgagatta atttatcgcc atcgcggcgt tatttcattc ggatttcatg
    21361 ttcaagcaac acctggttgt ttcagtcaac ggagtattac atgtccagaa ggcttcgcag
    21421 aacaaaaatc gttaccacgt taggcccagc aacagatcgc gataataatc ttgaaaaagt
    21481 tatcgcggcg ggtgccaacg ttgtacgtat gaacttttct cacggctcgc ctgaagatca
    21541 caaaatgcgc gcggataaag ttcgtgagat tgccgcaaaa ctggggcgtc atgtggctat
    21601 tctgggtgac ctccaggggc ccaaaatccg tgtatccacc tttaaagaag gcaaagtttt
    21661 cctcaatatt ggggataaat tcctgctcga cgccaacctg ggtaaaggtg aaggcgacaa
    21721 agaaaaagtc ggtatcgact acaaaggcct gcctgctgac gtcgtgcctg gtgacatcct
    21781 gctgctggac gatggtcgcg tccagttaaa agtactggaa gttcagggca tgaaagtgtt
    21841 caccgaagtc accgtcggtg gtcccctctc caacaataaa ggtatcaaca aacttggcgg
    21901 cggtttgtcg gctgaagcgc tgaccgaaaa agacaaagca gacattaaga ctgcggcgtt
    21961 gattggcgta gattacctgg ctgtctcctt cccacgctgt ggcgaagatc tgaactatgc
    22021 ccgtcgcctg gcacgcgatg caggatgtga tgcgaaaatt gttgccaagg ttgaacgtgc
    22081 ggaagccgtt tgcagccagg atgcaatgga tgacatcatc ctcgcctctg acgtggtaat
    22141 ggttgcacgt ggcgacctcg gtgtggaaat tggcgacccg gaactggtcg gcattcagaa
    22201 agcgttgatc cgtcgtgcgc gtcagctaaa ccgagcggta atcacggcga cccagatgat
    22261 ggagtcaatg attactaacc cgatgccgac gcgtgcagaa gtcatggacg tagcaaacgc
    22321 cgttctggat ggtactgacg ctgtgatgct gtctgcagaa actgccgctg ggcagtatcc
    22381 gtcagaaacc gttgcagcca tggcgcgcgt ttgcctgggt gcggaaaaaa tcccgagcat
    22441 caacgtttct aaacaccgtc tggacgttca gttcgacaat gtggaagaag ctattgccat
    22501 gtcagcaatg tacgcagcta accacctgaa aggcgttacg gcgatcatca ccatgaccga
    22561 atcgggtcgt accgcgctga tgacctcccg tatcagctct ggtctgccaa ttttcgccat
    22621 gtcgcgccat gaacgtacgc tgaacctgac tgctctctat cgtggcgtta cgccggtgca
    22681 ctttgatagc gctaatgacg gcgtagcagc tgccagcgaa gcggttaatc tgctgcgcga
    22741 taaaggttac ttgatgtctg gtgacctggt gattgtcacc cagggcgacg tgatgagtac
    22801 cgtgggttct actaatacca cgcgtatttt aacggtagag taagtacgtt gccggatgcg
    22861 gcgaaaacgc cacatccggc ctacagttca atgatagttc aacagatttc gaatattctg
    22921 aagcaaactt gaacttatca tcaggcgaag gcctctcctc gcgagaggct tttttatttg
    22981 atgggataaa gatctttgcg cttatacggc tggatttcgc ccggtttgcg agttttcagc
    23041 aattttagta tccaggtgta ttgttctggt cgcggaccaa caaaaatctc gacttcttca
    23101 ttcatccgcc gcgcaatcgt atgatcatcc gcctctaaca gatcatccat cggtgggcgc
    23161 acctgaatcg tcagacgatg cgtcttgcca tcataaatcg gaaacagcgg tacaacgcgc
    23221 gcacggcaca ctttcatcaa acgaccaatc gcgggcaacg tcgctttata ggtggcaaag
    23281 aaatccacaa attcgctgtg ctctgggcca tgatcctgat cgggtaaata atatccccag
    23341 tacccctgac gtaccgactg gatgaatggt ttaataccgt catttctcgc atgcagacga
    23401 ccgccaaagc gacgacgcac cgtgttccag acataatcaa aaaccgggtt gccctgatta
    23461 tggaacatcg ctgccatttt ctgcccttgc gaggccatca gcatggcagg aatatcgacg
    23521 gcccaaccgt gcggcaccag aaagataact ttctcgttat tacgccgcat ctcttcgatg
    23581 atctccagcc cttgccagtc aacgcgcggc tgaattttct ccggcccgcg tattgccaac
    23641 tcagccatca ttgccatcgc ttgcggcgcg gtggcaaaca tctcatcaac aatcgcttcg
    23701 cgttcagctt cactacgttc tggaaagcag agcgacagat tgattaacgc acgacggcgt
    23761 gagctttttc ccagtcgtcc ggcaaaacgt cccagccgtg ccagaatggg atcacggaac
    23821 tttggcggcg ttaaagcgat acccgccatc gctgctacgc ccagccatgc tccccagtag
    23881 cgcgggtggc gaaaggattt atcaaactca ggaatgtatt cgctattatt ttttttcgtt
    23941 tccatgcttt tccagtttcg gataaggcaa aaatcaatct ggtgatagtg tagcggcgca
    24001 acttgccccg caccaaataa aaaaagccgg tactgactgc gtaccggctg cgaatggatg
    24061 ttaattaatc aaaccgtagc tgcggcacaa tctctttggc ctgcgccagg aattcgcgac
    24121 gatcggagcc ggtcagccct tcggtacgcg gcagttttgc cgtcagcggg tttacggcct
    24181 gctggtttat ccatacttca tagtgcagat gcggcccggt tgaacgtccg gtattaccgg
    24241 aaagcgcgat acggtcgcca cgtttcacct tctgtcccgg tttcaccaga atcttgcgca
    24301 agtgcatata acgcgtggtg tagctgcgac catgacgaat agccacataa taacctgctg
    24361 cgccactgcg tttggcaacc accacttcac cgtcacccac tgaaagcact ggcgtaccct
    24421 gcggcatggc gaaatcaaca cctctgtgtg gtgcaacgcg accggtcacc ggattagtac
    24481 gacgcgggtt aaagttagac gagatacgga actgtttcgc cgtcgggaat cgcaagaatc
    24541 ctttcgccag accagtaccg ttacgatcgt agaatttgcc atcttcagcg cggatagcgt
    24601 aataatcttt accttctgaa cgcaaacgta cgcccagcag ctggctttgc tcacgtttac
    24661 catcaagcat ttctcgagac attaacaccg caaattcatc gccttttttc agtttgcgga
    24721 aatccatttg ccactgcatg gctttaatca ctgcgctcac ttcggcgctg gttaaaccgg
    24781 cgtttctggc gctggcaaca aagcttcccc cgacggtacc tttcagcaga ttgttaaccc
    24841 actctccttg ctgcatttcg ctggtcattt taaaaccgtt agcggcagta cggtcatagg
    24901 ttcgggtttc acgacgagac acttcccagg tgaggcgctg cagttcgccg tccgcggtta
    24961 atgtccagga gagttgttga ccgattttca ggttacgcaa ttctttgtcg gccgcagcca
    25021 gttgggtgat atcacccata tcaataccat actgattgag aatgctgctt agcgtatcgc
    25081 cagtggaaac aacatattca tgcacgcccg cttcaccggc gattttgtca tccagttcgt
    25141 cctggggaat ggcttcatct tcttgtgcag cttgatcaat cggctcactg gcttcaggta
    25201 agagcgaacg aatttcgttc tgttccagct caatggtttt gacaattggc gtggcatcac
    25261 ggtgataaac ataaggccgc cagacagcga cggccagagt aagaacggtg agcgacccca
    25321 acataacgcg gtgtggtcgc ggtaaattat taaacgccag ggcgacagag cgggctatct
    25381 gttgcacgta atcacttcct cattaatctc ctttcaggca gctcgcatac tggttggcta
    25441 attgactcag gaattctgaa tagcttgttt tacccagttt gatattcgtc cccaggggat
    25501 ccaacgttcc catacgaacg gatgtccctc gtgcgacgct ctcaacgacc gctggcctga
    25561 actgtggctc agcaaaaacg caggttgctt tttgctcaac caactgtgtt cttatttcat
    25621 gtaaacgctg cgcgccaggt tgaatctcag ggttaacggt aaaatgacca agcggtgtca
    25681 gtccaaactg tttttcgaaa tagccgtaag catcgtgaaa aacgaaataa cctttcccct
    25741 tgagcggcgc gagctcgtta ccaacctgcg tttcggttga ggctaattgt gcctcaaaat
    25801 ccttcaggtt ggcgtcaagt ttggctcgac tttgcggcat aagttccact aattttccat
    25861 ggattgcaac cgctgtagcc cgcgctatct ctggggaaag ccaaagatgc atgttgaaat
    25921 cgccgtgatg gtgatcttcg tcactttttt ccgcgtggtc gtgatcatca tcatcgccgt
    25981 gaatactttt catcagtagc ggtttcacat cttcaagctg cgcaatcgtt acctgcttcg
    26041 ctcctggtaa tttgcttacc ggtttttgca taaacgcttc catctccggg ccaacccaaa
    26101 cgactaagtc cgcgttctgt aagcgtttta catccgatgg gcgcagtgaa taatcatgtt
    26161 ctgaagcgcc gtcaggaagt aaaacctctg tttctgttac cccatcagca atggcagaag
    26221 cgatgaaccc aacgggttta agcgaagcga caacggcggc atctgcggcc tgtgttgcac
    26281 ctccccagag agcggcggat aatgctgcga aaagaagcgt ttttttatgt aacataatgc
    26341 gaccaatcat cgtaatgaat atgagaagtg tgatattata acatttcatg actactgcaa
    26401 gactaaaatt aacatgacaa gtctggtttc cctggaaaat gtctcggttt cttttggcca
    26461 acgccgcgtc ctctctgatg tgtcgctgga acttaaacct ggaaaaattt tgactttact
    26521 tgggccaaat ggcgcaggta agtcgacact ggtacgggta gtgctcgggc tggtaacacc
    26581 cgatgaaggg gttatcaagc gcaacggaaa actgcgcatc ggctatgtac cgcagaagct
    26641 gtatctcgac accacgttgc cactgaccgt aaaccgtttt ttacgcttac gccctggtac
    26701 acataaagaa gatattttgc ctgcactgaa acgtgtccat gccgggcacc tgattaacgc
    26761 accgatgcaa aagctctctg gtggcgaaac gcagcgtgta ctgttagcgc gagcattgtt
    26821 aaatcgcccg caattattag tgctggatga acccactcaa ggcgtggatg taaatggcca
    26881 ggtggcgtta tatgacctta ttgaccaact gcgtcgtgaa ctggattgtg gcgttttaat
    26941 ggtttctcac gatctgcatc tggtgatggc aaaaaccgat gaagtgctgt gcctgaatca
    27001 ccacatttgt tgttccggca caccggaagt tgtttccctg catccggagt ttatttcaat
    27061 gtttggtcct cgtggtgctg aacaactggg tatctatcgc catcatcata accatcgtca
    27121 cgatttacag ggacgaattg ttttgcgtcg gggaaatgat cgctcatgat tgaattatta
    27181 tttcccggtt ggttagccgg gatcatgctc gcctgtgccg cgggtccgct gggttcgttt
    27241 gtagtctggc gtcgtatgtc ttatttcggt gatacgctgg ctcatgcctc attacttggc
    27301 gtcgcgtttg gtttgttgct ggacgtgaat ccattctatg cggtgattgc cgttacgctg
    27361 ctgctggcgg gcggtctggt atggctggag aagcgtccac agctggcgat cgacacgtta
    27421 ttagggatta tggcgcacag tgccctgtcg ctgggcctgg tggtcgtgag tctgatgtct
    27481 aatattcgtg ttgatttgat ggcttacctg ttcggtgatt tgctggcagt gacgccagaa
    27541 gatctcatct ctattgcgat tggcgtggtc atcgtggtgg ctattttgtt ctggcaatgg
    27601 cgcaatttgc tgtcaatgac gataagcccg gatctggcgt ttgttgatgg tgtgaaatta
    27661 cagcgagtga aattgttgtt gatgctggtg acggcattga cgattggtgt agcgatgaaa
    27721 tttgtcggtg cgttgattat tacttcgttg ctgattattc ctgctgctac tgcgcgtcgc
    27781 tttgcccgca cgccggaaca gatggctggt gtcgctgttt tggtgggaat ggtggcagtg
    27841 actggcggtt taaccttttc cgcggtttac gatacgccgg cgggtccgtc ggtggtgcta
    27901 tgtgcggcac tgttatttat tctcagtatg atgaaaaagc aggccagcta atctatcgct
    27961 gaacacattt gtcggatgcg gcgcgagcgc cttatccgac ctacggttcg ctatctctgg
    28021 ctggcctgat aagacgcgaa cagcgtcgca tcaggcacac tgccaatgtc ggatgcggtg
    28081 cgagcgcctt atctgaccta cggttcgcta tctctcgtag gccggataag acgcgaatag
    28141 cgtcgcatca ggcacactgc caatgtcgga tgcgtcgcga gcgccttatc cgagctacgg
    28201 ttcgctatct ctggtaggcc ggataagacg cgaatagcgt cgcatcaggc acactgccaa
    28261 tttcggatgc ggtgcgagcg accaatccga cttacggcat ttctggcggc gttatgccaa
    28321 agtgattcca cgcccgcgtc gtcgccatac gcccacgcgg tgtacgctgc aaaaagcctt
    28381 gctgaatcaa ataaggttcc agcacatcct caatggtttc acgttcttcg ccaatggctg
    28441 ccgcaaggtt atccagacct accggcccac caaagaactt atcgattacc gccagcaata
    28501 acttgcggtc catataatcg aaaccttcag catcgacatt caacatatcc aacgcctgag
    28561 cagcgatatc tgccgagatg gtgccatcgt gcttcacttc ggcgaaatca cgcactcgac
    28621 gcagcagacg gttggcaatg cgcggcgtac cgcgagcgcg acgagcaact tccagcgccc
    28681 cgtcatcact catctcaagc cccataaagc gtgcgctgcg actgacgata tattgcagat
    28741 ccggcacctg ataaaactcc agacgttgca caatcccaaa acggtcgcgc aacggtgatg
    28801 tcagcgaacc tgcgcgcgtg gttgcaccaa tcagggtaaa cggcggcaaa tcaattttaa
    28861 tggagcgtgc cgccggacct tcaccaatca tgatatccag ttggtagtct tccattgccg
    28921 ggtacagcac ttcttcaaca actggcgata aacggtggat ctcatcaata aacagcacgt
    28981 catgcggttc aaggttagtg agcatcgcag ccaaatcgcc cgccttttcc agcaccggac
    29041 cagaagtcgt gcgtaaatta acgcccattt cattggcgac aatgttggca agcgtagttt
    29101 tacccaaccc cggaggacca aaaatcaaca aatgatcgag ggcatcgccg cgcagtttcg
    29161 ctgctttgat gaaaatctcc atctgtgaac gaacctgcgg ctgaccaaca tactcttcca
    29221 gtaatttagg gcgaatggcg cgatctgcta catcttccgg caaagtggta ccggcagaaa
    29281 tcagacggtc tgcttcaatc atcctttacc tcataacgcg gcgcgtaggg cttcgcgaat
    29341 taatgtttca ctgctggcgt cagggcgagc gattttgctc accatgcggc tggcttcttg
    29401 tggtttatag cccagcgcca ccagcgcagc aaccgcttcc tgttcagcat cgttggttgc
    29461 cgggctggca ggcgacgtga gcaccagatc ggcggctggc gtaaagagat cgccatgcag
    29521 acctttaaat cggtctttca tttcaacaat caagcgttcg gcggtttttt tgccaatacc
    29581 cggcagtttc accagcgccc ccacttcttc acgctcaacg gcattaacga actgctgcgc
    29641 tgacattccg gagaggatcg ccagcgccaa cttcgggccg acgccgttgg ttttgatcaa
    29701 ctctttgaac aacgtgcgct cttgtttatt gttaaaaccg tacagcagct gcgcgtcttc
    29761 acgcaccaca aagtgggtga aaacgatcgc ttcctgtccc gcttcaggga gttcataaaa
    29821 acaggtcatc ggcatatgca cttcatagcc tacgccgccc acttcaatta acaccagcgg
    29881 gggttgtttt tcaatgatga tgcctctgag tctgcctatc acatgacgct cctgcgtaat
    29941 gaatcaaaga taatgctgta tgataaaaaa atgctggata gatatccagc gaaggatgaa
    30001 gaaaacttgc gaggtgtctc gatgatctg
//
LOCUS       DAPHBJ010000035        29858 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.035, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000035 DAPHBJ010000000
VERSION     DAPHBJ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.035"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(85..1026)
                     /gene="fabY"
                     /locus_tag="RHZ76_001447"
                     /gene_synonym="yiiD"
     CDS             complement(85..1026)
                     /gene="fabY"
                     /locus_tag="RHZ76_001447"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HDV2812109.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1071..1508)
                     /gene="dtd"
                     /locus_tag="RHZ76_001448"
     CDS             complement(1071..1508)
                     /gene="dtd"
                     /locus_tag="RHZ76_001448"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDV2812110.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1505..2377)
                     /locus_tag="RHZ76_001449"
     CDS             complement(1505..2377)
                     /locus_tag="RHZ76_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HDV2812111.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2371..2970)
                     /gene="yihX"
                     /locus_tag="RHZ76_001450"
     CDS             complement(2371..2970)
                     /gene="yihX"
                     /locus_tag="RHZ76_001450"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HDV2812112.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3111..3998)
                     /locus_tag="RHZ76_001451"
     CDS             complement(3111..3998)
                     /locus_tag="RHZ76_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HDV2812113.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(3988..5337)
                     /locus_tag="RHZ76_001452"
     CDS             complement(3988..5337)
                     /locus_tag="RHZ76_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HDV2812114.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLILMALRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(5436..6869)
                     /locus_tag="RHZ76_001453"
     CDS             complement(5436..6869)
                     /locus_tag="RHZ76_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2812115.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASSTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGLL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            complement(6939..7949)
                     /locus_tag="RHZ76_001454"
     CDS             complement(6939..7949)
                     /locus_tag="RHZ76_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDV2812116.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(7982..8869)
                     /locus_tag="RHZ76_001455"
     CDS             complement(7982..8869)
                     /locus_tag="RHZ76_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDV2812117.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(8894..9412)
                     /locus_tag="RHZ76_001456"
                     /pseudo
     CDS             complement(8894..9412)
                     /locus_tag="RHZ76_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
     gene            9460..10829
                     /locus_tag="RHZ76_001457"
     CDS             join(9460..9940,9940..10829)
                     /locus_tag="RHZ76_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HDV2812118.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKIGTKRNIAISVDPEKAASALELSKDRRIEDLERQVRFLETRLMY
                     PKKAESLSSSHEKVKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKK
                     RISEIYHENRGRYGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRGE
                     VGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERP
                     VMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLD
                     NAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQ
                     TYMPRV"
     gene            complement(10856..11218)
                     /locus_tag="RHZ76_001458"
                     /pseudo
     CDS             complement(10856..11218)
                     /locus_tag="RHZ76_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
     gene            11391..12287
                     /locus_tag="RHZ76_001459"
     CDS             11391..12287
                     /locus_tag="RHZ76_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDV2812119.1"
                     /translation="MTKIACVGITVQDRIYSLLTLPDGGGKYQANHYLEIGGGPAATA
                     TVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            12327..13115
                     /locus_tag="RHZ76_001460"
     CDS             12327..13115
                     /locus_tag="RHZ76_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV2812120.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(13226..14491)
                     /locus_tag="RHZ76_001461"
     CDS             complement(13226..14491)
                     /locus_tag="RHZ76_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2812121.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASKNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(14593..15573)
                     /locus_tag="RHZ76_001462"
     CDS             complement(14593..15573)
                     /locus_tag="RHZ76_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDV2812122.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEASHLLNH"
     gene            complement(15581..16291)
                     /locus_tag="RHZ76_001463"
     CDS             complement(15581..16291)
                     /locus_tag="RHZ76_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HDV2812123.1"
                     /translation="MAENKSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(16508..18331)
                     /gene="typA"
                     /locus_tag="RHZ76_001464"
     CDS             complement(16508..18331)
                     /gene="typA"
                     /locus_tag="RHZ76_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HDV2812124.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            18704..20113
                     /gene="glnA"
                     /locus_tag="RHZ76_001465"
     CDS             18704..20113
                     /gene="glnA"
                     /locus_tag="RHZ76_001465"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HDV2812125.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            20287..21336
                     /gene="glnL"
                     /locus_tag="RHZ76_001466"
     CDS             20287..21336
                     /gene="glnL"
                     /locus_tag="RHZ76_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HDV2812126.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            21348..22757
                     /gene="glnG"
                     /locus_tag="RHZ76_001467"
     CDS             21348..22757
                     /gene="glnG"
                     /locus_tag="RHZ76_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HDV2812127.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAAHELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            22869..22979
                     /locus_tag="RHZ76_001468"
     CDS             22869..22979
                     /locus_tag="RHZ76_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HDV2812128.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(23169..24542)
                     /gene="hemN"
                     /locus_tag="RHZ76_001469"
     CDS             complement(23169..24542)
                     /gene="hemN"
                     /locus_tag="RHZ76_001469"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDV2812129.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(24731..25237)
                     /gene="yihI"
                     /locus_tag="RHZ76_001470"
     CDS             complement(24731..25237)
                     /gene="yihI"
                     /locus_tag="RHZ76_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HDV2812130.1"
                     /translation="MKPSSPSRSKGHAKARRKTREELNQEARDRKRQKKRRGHAPGSR
                     AAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELLE
                     TDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQED
                     MMRLLRGN"
     gene            25819..26451
                     /locus_tag="RHZ76_001471"
     CDS             25819..26451
                     /locus_tag="RHZ76_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDV2812131.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(26833..29619)
                     /gene="polA"
                     /locus_tag="RHZ76_001472"
     CDS             complement(26833..29619)
                     /gene="polA"
                     /locus_tag="RHZ76_001472"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDV2812132.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
BASE COUNT         7572 a         7701 c         7222 g         7363 t
ORIGIN      
        1 agttcttatt taatataacc gaaatagcca aggactaata taaatgacct gaatgagaga
       61 ggcagcaccg cccctctcca tcagctactc ttcttcgttc ccgccctctt catacgggcc
      121 aaatggcttc gcgggcagaa cgatatacgt gccttcaaac actgcacccg gcgtctcgtc
      181 gccaaagatt tcgacctgca tctgcacccg tgcttttcgt ccgcgcgcca gacggtcgag
      241 atcgccgctt aaggcaccga ggtcggctac cgcatgaggt ttaccgctaa tcggcttgct
      301 gtagcggata tgcgcatccg caagaataat cgttccgccg aggtggcgtt cacgcagcat
      361 cagccagata agtccccaac cggtgagcgt cgccagtgag aataaactcc cggcaaacag
      421 cgtatggtgc ggattctgat tgccggtttc tggcatggta gtgataaatt tttgcccggt
      481 atattgctga atgcgcacgc ccattttttc actaagcggg atatgttcgt accacgcctg
      541 ttgcagctgc gcgcaccagt cgccgcgatg cagaatgtca tccagagtgg cgacgggctt
      601 aatcatcaaa aaatggcgaa tcggcgtggt ggttggcgtg gtgatttctc cctgattaac
      661 aaaccccagc ttggcgaaaa actccaccgc gtcttcacgg gcgctacagg tcacgcgctt
      721 aacgccttcc tgacgcgcca ccgactccag ggtcatcgcc atcagcgtgc ctaacccttt
      781 gtcctgcacg tcgggatgaa cggccataaa gcgaatggac gcttcattgt cggcattaat
      841 atacagtcgg cctaccgcca ccagattacc ctgctcgtcg acgaccatct ggtgatgcgc
      901 catcgcatcc cacgcgtcgc gttccgaacc ttttggttga tgcaggggct tacgcaacat
      961 ttcccagcga aactgatagt aacgctctaa ttcttcttct gtttgtggaa cccgaaggtg
     1021 atacatagcg atactctctc ttgtttcccg tgacaaccct ggaagctggc tcatacctgc
     1081 aaccagaatg tcacggggcc atcattgacc agcgatacct gcatatccgc agcgaagcgt
     1141 cctgtttgcg tgttcatctc ttgctgacgg cagcgttcga cgaaatagtc atataacgcc
     1201 tctgcgcgat ccggtgatgc acctttggag aaacttgggc gcatcccccg ttcggtatct
     1261 gcggcgaggg taaactggga aaccaccagc acactgccgc ccgcctgttg cacgttgaga
     1321 ttcatcttgc cttcggcatc gctaaagatg cggtagccga gcacacgctc gcacagacgg
     1381 tttgctttct gttcgtcgtc atccttttcg acacccaata acaccaaaag tcccgcgcca
     1441 atttcgcccg tcacttctcc ctccacggtg acgctggcac gggttacgcg ttgaattaat
     1501 gcaatcatgg ttcgtcgtct tcttcttgtt cagcagcttg ttttagtttg cggtattccc
     1561 cgagagtgac agtaatttcc gcgccaagca agacgataca ccatgtccag tagacccaga
     1621 caaagagaat ggggatcacc gccagcacac cgtaaatgag ctgatatgac gggaacatgg
     1681 tgatataaag cgcgaaacct ttctttcctg cttcgaacag gagtgcagca acaaacgcgc
     1741 cgacaatcgc gtcgcggtta ggtacgcgga tggtaggaac aatgctgtac agcaaccaga
     1801 aggagatcca cgacaacagc agcggaaaaa tacgcagcac gttgtcgatg acagtattga
     1861 gatcgctcgc ccagcgcagg gagagcaaat aggaactgat cgccagacta gcccctgcca
     1921 gcagcggccc cagcgttaaa atcatccagt acacggcaaa cgagtaaatt tttggtcgcg
     1981 ctcgtttact gcgccagatg gtattcaacg cgctatcgat ggagtacatc aacaataacg
     2041 cggtgacgat cagcccgcac gcgccaacgg cggtcatctt gttggaattg gcaacaaatt
     2101 gttcgatata ccgctgaata acatcgccag tagcaggcag aaagttggca aaaataaagt
     2161 gacgcaactg aatgctgacg tcggaaaaca tgggaaaagc ggcgaaaagc gcaaaaacaa
     2221 cggcaaccag cggcactaat gagagcaacg acacataggc aaggttacct gccagggttg
     2281 tcatgttgtc ctcatcaata cgttgccaga gtagttttag ccaggcccat agtggacggg
     2341 tacggtgcct ggctttgtcc tgaatggttt ttagcataac accttcgcga aatagtccgg
     2401 gatggtggtt ttatctttca ccagaatact ggtaatgcct agctgattag ctccttctat
     2461 attatcggcg ttatcgtcga aaaagaccgt atcgctgggt gaaaaacctt ccgcctgcaa
     2521 aacatgctgg taaattcgag cttcaggttt gcgcatcccc agatcttgcg acagatagat
     2581 atggtcagca gcatcacgaa tttccgggta ttcttccggc cagaaggtgg tatgcaggcg
     2641 gttggtattg gaaagcacca ccacgcgatg cccctgctca cgcagtttat gcatgatggc
     2701 gatcacttcc gggcgcagcg caacaaacac cgcctgccag ccgtgagaga actgctcgta
     2761 gcttagcggt agagccatct catgacacag cgcctctgcg aacgcttcgt cgctaatttc
     2821 cccacgctca tgctgatgaa acgcctcccc catatgaaaa ctcttcttaa gcgatgccag
     2881 cggaatacgc gttaaatcgc tccaggctcc cagcacacgg ttaaagtcga tatcgacaat
     2941 cacattacct aaatcaaaga tatagagcat ttttgcctcc tttcgcgcca caagaaatta
     3001 actgtagcgg gaaaggatgg gtttggctat gtggcaataa gggtaatctt gtgatgctga
     3061 ttgcgaaaaa gcagagtaat ttcactggct ttttcgcaat agatgaatgg ttaagattca
     3121 ggtgtaaatt gccaacgagt ttcgatgacc agcggcctat ctggtgtcac caattccggc
     3181 gttagattta ttgcgttagg tgcctgagtc agcgggttaa tacaggtggc atcgggctgt
     3241 ttatcaaata ccaccagact gtttgcagaa gagatcatgg tcatcgcgag tttaccaggc
     3301 cacagtaact taaccttcac cccgtcataa aaaccaaaac agtcatccca gggaccggct
     3361 tgaggagaga tacgatttcc agttggcagt tcatcactac ccgcttcttc ttgccagtcc
     3421 gcatcaaaaa gcacctgcaa cgattctgta ttttgcggag tcagcttttt cgcaaaccac
     3481 ggatgccagc ctgcggaggc cgggaatgta tcggcatatg aatggacttc cagttgcaaa
     3541 accagcgcat cgttctccag caaaaaagtc tgaatcactt ccccttgcca gggccacggt
     3601 gaagccaggg gcatacgtaa agtcagggaa tctgctgttt tgtcgatgat ttcccacgtt
     3661 gagtaacaag ccataccatg caacgcatgt ggtggtttat tggcgggtaa agaataacat
     3721 tgcccaccag cattcagcgt cgcattcccc agtcgccctg cccacggcac cattgggaaa
     3781 cagccatact gaaaagctct gcgctgcggc tgccattggc gtaatacttc agaaccaaat
     3841 gcctttaacg aggttatcct ggcaccatca tgcggataaa tctgcaggtg aatattgcct
     3901 ttttccagcg tcactaactc tcctacaggc ccaggccatg gcgcgtcaaa cgcctgttgt
     3961 agcgccaggt aatgctcgtt ttttcgttca gttttcatga gattgttccc tgatgtagcg
     4021 gtaagaagcg gtttcttgtt catcggtcgg ctcaatcagc cagacgcaaa caaaggaaag
     4081 aactgagaca gcaaataaaa acagagcgac ataccacggt tttcctcccc ctagcgccag
     4141 caaacttgag gcgataaggg ggcttagtcc gccaagaatc gcggaaaatt gataagcaaa
     4201 agaaaggccg gtatatcgta ctttggtacc gaacatgcgg gtgaaaagtg ttggctgcac
     4261 ggcaaacatc atcgtcggac ctaagttgta gccgaggatc atactgcacc agatgatgag
     4321 cgtacttttg ctatccagta ataagaagaa aggaaacgcg aacaggacac aaaatcctgc
     4381 tcccgacaaa tacagacttt tttgacctat acgatctgat aaccatccaa ccaatggaca
     4441 actgaaaata ccgatcgccg aggcaatcaa catacccgtc aggggaatat ctcgcgacaa
     4501 tgctaattgg ctggaaatat agacaatacc aaaggcgtta ataatattag aggagacact
     4561 ttccgccagc ctcgcaccca gtgccaataa aatatttcgt ggatgttttt tgaataattc
     4621 aatcaacgga ggaacggact tttctttatt attcgtcgtt ttttgctttt gaaaatccag
     4681 cgtttcttcg gtatgcaggc ggataaacag cccaacgatc aacatcaccg cagaaagcca
     4741 aaacggaatg cgccatcccc aggagagaaa ctgctccgaa gtaagaaaat ggttacagag
     4801 agcaaaaata ccggttgcca gcataatacc cacagacgca gccgtttgcg ggagtgaacc
     4861 taaaaatccg cgtcgggatt cgggcgctga ttctatggtc atcaatgccg ctccgccata
     4921 ctcgccacca agaccaaagc cctgaattaa acgcagcgcc attaatatga gcggtgccca
     4981 gatcccaatt tcctgatacg tcggaataaa accaatcaaa aatgtggagc aaccgacaat
     5041 agccagtgtc cagataagtg ttatttttcg cccaatttta tcgccaaaat ggccaaagac
     5101 gataccacct aaaggacggg tcaaaaaacc aacgccaaag gtagcaaagg cggctataag
     5161 acctataaaa ggatcgctgt ctggataaaa cagcggtgca aataccagtc ctgctgccgt
     5221 gccaaaaata aagaagtcat accattccat taacgcgcct gcgaaactgc ccataacgac
     5281 acgtctcatg gctttaccgg aaagcccttc acgcggtgcg ggtaactttt cagacataat
     5341 tttcctctcc tggtaggcga gaaatctggg atacgaacca ctaaacgtgg gcagtttgaa
     5401 ttaatgcgca tcggtttaag ccgatgcgca ttaatttatt tcttcaattc agccagaacc
     5461 tctgcatcct cagcatcaac atttgcaccc agtggttccc aattaatcaa tacgcaggtc
     5521 aggagcccca agaatggcac gattgccaac cagtaaaacg cttcagtttt caacgccccc
     5581 cacagcatcg gccagaaaat gagtcctgca attgcccctg tgcgcataat agcgcgggca
     5641 aatccgaccc cagtagggcg aatagcggga ggataagaga gtgtggcaat ggtcatgccc
     5701 agcccaccag gacccgctga atggaagaaa ataattgatc ccaataaccc taaagacagc
     5761 catggattag tcgttgccag tgcgcctaac gacagcaatg ctaaggtcac aacagcaaat
     5821 ccatacatcg actgtcggcg agtacccagt cgttggagaa taaacgagcc gatcatcccg
     5881 cctgtcaccc cacaaagggc attcacgacg gcagaacccg tcaacgcacc agtaagcccg
     5941 ccacttatta ttcccgccaa cgtaagagga agataaacac ctacggcgtt atattgccac
     6001 gcctgcatgg ttgccacgac acagccaaga atggtgcgtt tgcggtaacg atcattaaat
     6061 aagatgccat atccaccact gtatttattc tctgcttttt ccgagaattt cgcttctgtt
     6121 gtactcgatg ccgcaacgcg agcctgaaca ccataacgtt ttcccagaat gtcgcacgct
     6181 tcctggtaac gcccgacgcg tgcggcccac attgcggatt caccaataaa gaagtaacgc
     6241 aaaatcatga agatgactgc aaaaatagct ccgatgaata ttccgtaacg ccacaattgt
     6301 tcttcagcga tacccgacag aagcagcggc aagagcacca aataaacaac agttgtcgaa
     6361 acataccagg ccatttgcca gaggttgacc gacgttccgg ttttcttcga cgttgaacca
     6421 cgaagctctg ccacggcatt ggttgccaaa gggaagtcga tgcctaaacc aaagcccatg
     6481 atgacgcggc acaccagcac ccaccagata ctgggcgcaa tagctaccgc agcagccccg
     6541 atggtgcaca gccccatgcc aataataaat gctcgctttt gcccaaagcg gtttattact
     6601 cggttcgcta ataaaccgcc aattaacgcg gcgattaata ctgacgcatt aacggttgcg
     6661 gcaaggcctg gagaaatgcc aaattgagcg gcaatgtatt tattaccaaa ccccaccatc
     6721 gccgcctggt aggcatcaat caatatgccg cccaaagcaa taaaaataac gccgatactg
     6781 gtgttgattg cagagttctt attaacaata tcaattacgt cctgtggccg actaattgaa
     6841 taagttgcag gactggtgat ttgactcatc gcagtgtcct ttttaattgt tgtagggtgg
     6901 cgctgacgtg acgcagcgcc agacagagat ttagaaaatc attccggcgc aaattgcagc
     6961 atgaccttta tggagtcatt ttgctgcgcg cgttcgaatg ccgccgctgc atcctcaatg
     7021 gcaaataaat cggtaaccag ttcactggta tcaaaccgcc catccgccag ccattcgata
     7081 acagcatcga aatcttcagg gatgtacatg ccagagttga gcaagtcacg ctcgaaacgt
     7141 tgcatcatcg gtaatggtat ttcacgcggc ccacttggta cgcccatgca cacgacggtg
     7201 cctccggcat atacacgggt acatgcatcg ttaagcgtgg cctgcgctgc aacgacatca
     7261 atcgcccccg taaatcgcac atctgcggcc agctcacctg gtgcccaaac ttctgctgcg
     7321 cccagcttta acgccagcgc gcgtttggca gcgtcaggtt caatcacggt aatcttacct
     7381 gcccccataa tgcgcagtgc ctgcacgatg gataagccta tcgttccggc accaatcacc
     7441 agtacgcttt ccagagaagc tttcggcatt cggttaacgc agtgacgggc acaagccgcc
     7501 ggttcggcaa aggccaacac ttttagcggt aaagaggctg gtgcgacatg gcaattacgc
     7561 gcaggaacaa tgtgttgtga gcgagcaaag cccggtgcgc gaaaaccagc aacctgcggt
     7621 ggttcacaaa gattaaaacg tcctgctttg caggctcggc attccatgca agccatgatg
     7681 ggatcgacca caacatgatc gcccggctgt acatttttaa cgtcgctgcc aacttccgta
     7741 acgcgcgctg caatttcatg accggggacg acaggtggtt tagcaaacgg atgaccgcct
     7801 ttcagaacat gcagatccga accgcatatc ccatagaaca caggcgcaat ccgcacttcg
     7861 tgcggtttaa gttcctggtg tggtacttca acatattgag tggtgacttt cccaatatct
     7921 gaaaataaaa cctgggtaat tttatccatt gttattctcc agaatcgata tccatagggg
     7981 attagcgttg cgcgatacct tcatcgacaa tttctgccaa ccgttgtaaa cgaggaacag
     8041 agacatctcg cagcatagtt tgtggatctt gtgcaccaac aaccgacgcc caaaccgctc
     8101 gtccagccag gaagcctgac gcaccacctt tcatggcaat acttactgca cggccaaaaa
     8161 tatcggcatc aacgccagaa gaaagaatta cccacggcat cttcatctga tcgttaagtt
     8221 gctgacaggc ggcaagtaag gttttttcat cgcctttgcc ccctaatggc atctcggctt
     8281 tgtaaagatc agcctctgtt ccaccaagct ctgcggcggc ggcaacaatg gcgctttcac
     8341 gatcaaagtc ccaaccgcga cgaggtggac gcaccacagg ttcaataatg ctaaccagcc
     8401 cggcactacg gcagcgacga acaaatttat caaccattgc cagacgttcc tctgcgggtt
     8461 catcttcacg ccagagtaca agtaatttca ttgccttagc ccccatttca cgggctttat
     8521 gcgggtcgac ggacatatct atttcaacgc tatcaaccgg gatgccatta cctgggataa
     8581 aaagatcggc agcgacaatc aggccgcaag aattagcaac agcgccctgc tcgactattt
     8641 gctcaagaca aaattgttta tccgccagaa cggcagaagc ataaggagaa aggattcggg
     8701 tcgcggcaac tttaaaatcg gtgagtacac tgtcggcgat gggttttggc tgccctgctg
     8761 ccgcaaacat caagcgcatg gcctcacgct gatcgacagc taacatggca aatccaccac
     8821 ctgggcggga gatatctttc agcgtaaatg gggtgaagtg ggatgacata gaaactcctg
     8881 ttaatagata aaattaaaga ccagcctgct ggcgaacctg atttagcagg gcactccagt
     8941 cctgccgccc tttgccgtct tgacttgctg cagcgtaata actttctgct gcaatcccca
     9001 tattgagcgg tgcccccgtc tcccgagcga cgtcaactgc aatacgcaga tctttcaacg
     9061 ccagatccac catgaaggcg ggtgaaagat cgcctttcag cactttgcaa ggccaggtgg
     9121 tcgtaaaatg tcctttccct gctgccgtac cactcatcac cttaattgcg acatcaagat
     9181 ttaatcccag tgactcacat aacacagcgg cctcggagga cagcgcatta agcgcaatgc
     9241 tcatatagtt gttaattagc ttcacgcgga tccccattcc tgggccgcca gcctcgacca
     9301 gctcattacc catgcacatg agaatcgggc gtgcacgtgc aatctgctca tcagtgccac
     9361 ccgctaaaat cagcaatgtg ccatcaatcg cattcacgga cgttctgccc actgtactgc
     9421 acccattttg ttggacgatg aaatggaata gcccctaata tgtcaaagcc aaaataccct
     9481 tttgaaaagc gccttgaagt cgtgaatcac tacttcacaa ctgatgatgg ttacaggatc
     9541 atctcggcac gttttggtgt cccccgaacc caggtcagga catgggttgc cctctatgaa
     9601 aaacatggag aaaaaggttt aattcccaaa cctaaaggcg ttagtgctga tccagagttg
     9661 cgtattaagg tcgtgaaagc tgtgatcgag cagcacatgt cccttaatca ggctgctgct
     9721 cactttatgc ttgctggtag tggttctgta gccaggtggc tgaaggtcta tgaagagcgc
     9781 ggagaagctg gtttacgcgc gctcaagatt ggcaccaaaa gaaacattgc aatatcagtt
     9841 gatccagaaa aagcggcatc agcattggag ctgtcaaaag accgacgcat tgaggatctt
     9901 gaaaggcaag ttcgatttct tgaaacgcgg cttatgtatc taaaaaagct gaaagcctta
     9961 gctcatccca cgaaaaagtg aaagtactca acgagctaag gcagttttat cctcttgatg
    10021 agcttctcag ggctgcggag ataccgcgca gtacgtttta ttatcatcta aaggctctca
    10081 gcaagcctga caagtatgcg gacgttaaaa agcgtattag tgagatttat cacgagaata
    10141 gaggccgata cggataccgt agggtaacgc tgtctcttca tcgagaaggg aaacagatta
    10201 accataaagc tgttcagcgc ctgatgggaa ccctctcact taaagcagcg attaaggtca
    10261 agcgataccg ctcttacaga ggagaggtag ggcaaaccgc ccctaatgtt ctccaaagag
    10321 atttcaaggc tacgcggcca aacgagaagt gggttaccga tgttactgaa tttgcagtca
    10381 atgggcgcaa gctgtatttg tctccagtaa tagatctctt caacaacgaa gttatttctt
    10441 acagcctttc ggaaagacca gtgatgaaca tggttgagaa tatgctcgat caggcattca
    10501 aaaagcttaa tcctcacgag catcctgttc tgcactctga ccagggatgg cagtatcgta
    10561 tgagaagata tcaaaatatc cttaaagaac atggtattaa acaaagcatg tccagaaaag
    10621 gcaattgtct ggataatgct gtggtggagt gtttctttgg aaccttaaag tcggagtgtt
    10681 tttatcttga tgagttcagt aatataagcg aactgaagga tgctgttacg gaatatattg
    10741 aatactacaa cagcagaaga attagcctga aattaaaagg tctgactcca attgaatatc
    10801 ggaatcagac ctatatgcct cgtgtttaac tgtccaactt tttggggtca gtacacacgg
    10861 gggcgtccat catactgatg cctttttctt gtaactcccg tattaatgca tcggtctgta
    10921 agggatgaat agtcgacata tcaatcaata acgcgtcaga tgaaacagcc tcacagacgc
    10981 ctttttcccc caacagaacc tgtcgaacaa tgtcaccatt gggaagcata gtgatgataa
    11041 attcagcgcc tgctgccgct tccgctgggg tttgtgccgc agtggccccc tgcgtaacta
    11101 gcgcatcgac ggcctgtgca ttgacatcaa atacctgcaa tgaatgccct ttttgcaata
    11161 gattgttagc cattgggcta cccatctgcc ccagcccgag aaatgcaatg gttgccatga
    11221 atactctcct gtctgacatt gacaaaataa tgacaattga aaattcgttt tcgtctgttt
    11281 ttgagcatat ttgtaattta tgttcaaaaa cacgacacgc atcactaatt tgatcatttg
    11341 tgtcatttaa attgaccgaa gacgacaaaa ttaaacagga caagacaaat atgaccaaaa
    11401 ttgcatgcgt gggtatcaca gttcaggaca ggatttattc tttgctcact ctgccggatg
    11461 gcggcggaaa atatcaggca aatcattatc ttgagattgg tggcggcccg gcagcaacag
    11521 ccactgttgc aatcgcaaag cttggcgtgg aggttgattt catcggtcgc gtcggcgatg
    11581 acagttgtgg aaatacctta cttgcagaac ttgagggttg gggcgtcaat accgcatttt
    11641 gccgccgata tcctaacgcc cgttcatcgc aatcagcgat cctcgttgat cagcatggcg
    11701 aacggattat cgttaattat ccaagtcccg atctcggtac tgatgccgaa tggttagaag
    11761 cgatcgattt ctctcgttat gatctgattc tggccgatgt tcgctggcat agcggtactg
    11821 aaaaagcatt ctcgctagcg cgtcttgcgg gtgtgacaac gcttttagac gccgatatga
    11881 caccgcagga tatttctcca cttgtcgcgc ttgccgatca cgccgtgttc tcaacaccag
    11941 ggctgaaacg catgactggc ctgcagtcac ctgaagaagg tttgttccag gcaacgacac
    12001 aaacagcagg taaagtgtat gttacgctgg gttctgaagg atcgttgtgg attgaagatg
    12061 gtcatttgtg tcaacaggag gcattttctg tcaatgtggt tgataccact ggagcaggcg
    12121 atgtttttca cggcgcactc gcggtagcgt tggcagaaaa aatgccaaca aaagaggcta
    12181 ttcgttttgc cagtgcggtt gccgcgatga aatgtaccca gcctggcggt cgggccggca
    12241 ttcctaatcg tgagcaaacc gaatcatttt tgtcacttta tgcgtaaaat ggtaaacatc
    12301 taccgaggag gggcaaggga gtattcatga gtatcatcga agtgacaggt aatccacgcc
    12361 atgaccaact cgttcatcta atcgcagaac gcggttatat gaatattgaa gaactggcac
    12421 aactgctgga tgtttcaacg caaaccgtgc gccgggatat ccgaaaactc agtgagcagg
    12481 gcttgatcac ccgtcatcat ggaggggccg ggcgcgtctc cagcgtcatg aataccgctt
    12541 ttgagcaacg ggaactttcg ctcaccgccg agaaacgggc gatcgctgag gcagtcgccg
    12601 attaccttcc cgaacgctgt accgtcttta tcaccatcgg tacaaccgta gaagccgttg
    12661 ccagggcatt actcaaccgg cgtgatttac gcattatcac taacagccta cgtgtggcac
    12721 agattcttta taagaatcag gatattgaag tgatggtgcc gggaggcact ttacgcgctc
    12781 ataacggcgg gattatcggc ccaggagccg tggattttat tgaaggcttc cgcgcagatt
    12841 atttaatcac cagtataggt gccattgaac atgacggcac cctactggaa tttgatgtaa
    12901 atgaagcgtt agtcgcgaga acgatgatta aacatgcgcg gaatacattg ttagtggccg
    12961 atcatacgaa gtttgccgcg tctgctgccg tttcaattgg caatgcacgg aatgtcaggg
    13021 ctttttttac agatgccccg cctcccaatt ctttctgcca gttgttaagt gaagagaatg
    13081 ttgaactggt ggttgccgag caggaagttt cctgaaattg aaacttcgaa aaatccgctg
    13141 gggattcagc ggattttatt ttgccggatg cggcctacgg ttgatacggt tttcgttggc
    13201 cggattaagc acatccggcc attaattaag cttcagccgc cagcatctct ttcttacgtt
    13261 ggctaatacg cttaaccagc attacggaaa ccaccgcacc acagaatgcg aagcaggcca
    13321 taatgccaaa cacgattttg tagccaataa tccccggatt taaatccaga atgtagccat
    13381 acagactgaa gcagaacatc gccggggcgt aaccaataaa gctacccaac gccatcgccg
    13441 cgcctgtttt attttcagca atttttgctt cgccgatagg tgcaaaaaat acggctcgct
    13501 gtgtaaagac tatcgcgcca aagcccagcg tacatgccat ccctaaatag accggcatac
    13561 tttcgtgcgg cagcataatc aacagtacga gcgcagcggt actgatgata aaggtgtagc
    13621 atagatattt actcggcgat ttcaggatct tatctgaaat catgccacca atcggtccac
    13681 caatcatttt cagacagtat tggttaatga tgccgtaagc ccccaccagc gcaacgggca
    13741 atagatagat gtttttcagg aatggaatga agaatgtcag gccacagtaa accgcgtaaa
    13801 cgaagaagac gttaaaagcg ataagccaga tagttttgtc tttcagcacc gaggtcatac
    13861 tggtgttttt cgatgctccg tcttcttttt tcacctcgac ggacggtgcc tcttctttgt
    13921 cattcagaac aaagaaaatg ataatgccta cggcaatcac aataagggaa tagaaccaga
    13981 tgcctgcttt aaaacccaat aagccactgc caaaccaggt aaatactgcc aacgcagaaa
    14041 atgccaccac ggtatcgaca atgccacgcc ctgtttcgaa gaagccaaac aaccgacctt
    14101 gttgttcgct attgcccaat cgacttaccg acttgagcaa gaccggccag ttcatcatgt
    14161 cgcaagtaac gccaaatagc gcccagacaa agaggatgcc ccaatacccc ggcattgtcg
    14221 tcaaataaac acccagtaat cctgtcgcaa tgagtgagaa tgacatggtg tatctgcgcg
    14281 gtagtttatc ggcaaaataa atagacagaa aaaaacccac tgtcgtgaca aatgagttta
    14341 ccgacatagc attaccaatt tgaccattgg tcaaatggaa atattcctgc atcgggatat
    14401 aaaacgcatc tttcagcgac ggtaatttat aaattgtacc gccacacagt gttaatagac
    14461 taaataaggc ccatttcttt ttcgtgagca taacatcccc taagcgattt ataaaatcac
    14521 tgttttaaag gcaataagat atcgaccatt cttatggaat ggttttatta actgcgtatg
    14581 agttaaaact tattagtggt tcagaagatg gcttgcctct tgtttgattt gttgtagtac
    14641 gttacgcaca tgatttatct gattaatcgc atcttctttt tctttacgta accgcgcatc
    14701 cagtaaatga ttttctggta gtggtgtttc actcatctgg cgataaggga tccacggatt
    14761 atcatcttcg tcttcaaagc ggaacgccgg agcataataa tcaacctctt cttccaggcc
    14821 atatgccgtc acctgcggct catcatcgcc caggcagata aggtgcgtta ataacgcttt
    14881 gaagggcata tctccctgac ctgaaataca ggctttatgg cccagaccac cctgttcttt
    14941 aacaatcaat gcatctttga tatgcacctg ggtaatatgc ggcgccatcg tttttaaagc
    15001 gtcgatggga tgctcatttg cattgatcat gttcgcaaaa tcaaacagta aggaaagaga
    15061 ttccatctca ctttctttga ccagcgacac cagctcatgg ctttttaaat cttcatgctg
    15121 ttcgatcgta aaagtcaggc cgctgtcctg atacgtttcc cgtacatagg caatgtcgtt
    15181 agcgataatc gataatacgt cgcgcaaatt accttcataa cgtggataaa aacgtacgga
    15241 cgatgcccca gttttcaacg cgatggcgac ggcttcgtca atagatgcct tatctgaggc
    15301 gctggtttca atatgaatat caaggttcag acggcgggct ttatctccaa aggcagagag
    15361 ttctttatcg tccatattac caagagaaaa acgctcgcca tccagaacat ggattttgac
    15421 gccacgtaga ttattttcac ttgcgatatc aagtaaatca gcaggcaaca ctctttccag
    15481 ccgcatattt aaatgaaagg cataagcatg taaataaaga ggaagagtgt cgacacgttg
    15541 tagaatcttt cttgcgttat ttattgtcac cataaaacac ctacagatta atgatgaggt
    15601 ctacggcctc aaatcgccaa tattcaatat ctatctggac aatattgcct tgctggtcgg
    15661 cgcgatgctt ttcaattaag atcgctggca tacccgcaga accgccaaga tacttgctga
    15721 tatcgcccgg cattctgacg ggtttgaaaa tcaatttttt tactaccgtc tctttttggt
    15781 agcacttttc ccagactgca gaaaatgagt ggttttcaag ttgttcaata aaacccggag
    15841 caacttcagg attaatatat gtttcgtgat agaaaacttt atgtccttcc agcgcccccc
    15901 agccggtgat gcgatagagt tcagttgatg gtgtcactgc aatcagtggc gcgagcgtct
    15961 cgggaatatc gctggtacgg tttttctcgg taaaccccca ggaaggctct cgtccttgct
    16021 caattgcggc ccgctgaaag ctcgctgaca gctccggact gtaattaaat cgcggctggg
    16081 tcacgaacca ccccttacga tccttacgga agattttgga ttctgcctcg aggttcaaca
    16141 acgcctggcg cagcgtcata cgtttaatgc ccagcagttc tcccagctct ctttctgaag
    16201 ggagttttcc acccggcgtg gtgatgccat ctttcaacca ccgatccagt ttctcttttg
    16261 cattttctac ggtggattta ttctccgcca ttgtttaatc cttttggttt aaaccagatg
    16321 aagcatctta cgcttacgcc aaaaaagaaa gcaattaacg gcgtatgact ttgcagataa
    16381 ttgtgacaaa gctctctatt gacgtaatcc cagacttata agggagatac tcgcattggt
    16441 gtgcgctcac gacaggtaaa aaaaaacctg ccagcgatgg caggtttttt ataactaaaa
    16501 aacgaaatta atcgtctttc ggtgcgcggt tggcgcgacg acgatcgttt tcagtcaggt
    16561 gacgtttacg aatacggata gaggtcggag tcacttctac cagttcgtca tcatcgatga
    16621 actccagagc ttgttccaga gtcatgcgga taggcggaac cagaacaacg gcttcgtcag
    16681 taccggaagc acgcatgttg gtcagtttct taccagtcag gcagtttacc gtcaggtcgt
    16741 tagagcggct gtgaataccg ataatctgac cttcgtaaac ttctgcaccg tgaccgagga
    16801 acagcttacc gcgatcctgc agaccgaaca gcgcgaacgc gaccgcttta ccctgaccgt
    16861 tagagatcag tacgccgttc tgacgctgac ccacttcacc cggacgtacg tcgtcgtagt
    16921 ggctgaaggt ggagtacagc agaccagtac cggaagtcat ggtcatgaac tcagaacgga
    16981 agccaatcag accacggctt gggatcacgt agtcgagacg tacgcggcct ttaccgtctg
    17041 gattcatgtt tttcaggtcg cctttacgtt cgcccagcgc ctgcattaca gaaccctgat
    17101 gctgttcttc aacgtccaga gtcacgtttt catacggctc ttgtttacga ccgtcgattt
    17161 cacggaagat aactttcgga cgggataccg ccagttcgaa accttcacga cgcatgtttt
    17221 cgatcagaac agacaggtgc agttcgccac gaccagaaac gcggaacgca tcggcgtctt
    17281 cggtttcttc tacgcgcagc gcaacgttgt gtaccagttc tttgttcaga cgatccagga
    17341 tctgacgaga cgttacgaac ttaccttctt taccgcagaa cggcgaggtg ttaacgcaga
    17401 agaacataga aacggtcggc tcatcaacgg agagtgccgg cagcgcttca acgttttgcg
    17461 tgtcgcaaac ggtgtcagaa atgttcagtt cgccaaggcc cgtgatcgca acgatatcgc
    17521 cagcttccgc cagatcggtt tcgatacgtt ccagaccgag gtggcccagc actttaccga
    17581 ctttcgcgtt gcgggttttg ccttcgctat cgatgatagt gacctgctgg ttcggcttca
    17641 ctttaccgcg cttgatgcgg ccaatgccga taacgccaac atagctgttg taatcgagct
    17701 gagaaatctg catctggaac ggaccgtcaa ggtcaacgtc cggcgcagga acgtgatcaa
    17761 caatcgcctg atacagcggg gtcatgtctt ccgccatatc ttcgtggtcc agacccgcga
    17821 taccgttcag cgcagaagcg taaacgatcg ggaagtccag ctgctcgtcg gtcgcgtcga
    17881 ggttaacgaa cagatcgaat acctgatcca caacccaatc aggacgcgcg ccagggcggt
    17941 caactttgtt gataacaaca atcggcttca ggccgtaagc aaatgctttt ttggttacga
    18001 agcgcgtttg cggcatcggg ccgtcaaatg cgtcaaccac cagcagcact gagtctacca
    18061 tggacattac acgttcaact tcaccaccga agtcggcgtg ccccggggta tcaacgatgt
    18121 tgatacggta atcattccat ttgatagcgg tgtttttcgc gaggatggta atcccacgct
    18181 ctttctccaa atcgttggag tccatcacgc gctcttgggt ttcggcacga gagtcgaacg
    18241 taccggattg ttggagcagc ttgtctacca gggtggtttt accatggtct acgtgcgcga
    18301 tgatggcgat attacgcaat ttttcgatca caactttgcc tcaggcatta gaaatagcgc
    18361 gttattgtac acggattaat cgcactacaa aacaggatca caaacatcct ccgcaaacaa
    18421 gtattgcaga gtccctttgt gatcgctttc acggagcata aaaagggtta tccaaaggtc
    18481 attgcaccaa catggtgctt aatgtttcca ttgaagcact atattggtgc aacattcaca
    18541 tcgtggtgca gcccttttgc acgatggtgc gcatgataac gccttttagg ggcaatttaa
    18601 aagttggcac agatttcgct ttatcttttt tacggcgaca cggccaaaat aattgcagat
    18661 ttcgttacca cgacgaccat gaccaatcca ggagagttaa agtatgtccg ctgaacacgt
    18721 actgacgatg ctgaacgagc acgaagtgaa gtttgttgat ttgcgcttca ccgatactaa
    18781 aggtaaagaa cagcacgtca ctatccctgc tcatcaggtg aatgctgaat tcttcgaaga
    18841 aggcaaaatg tttgacggct cctcgattgg cggctggaaa ggcattaacg agtccgacat
    18901 ggtgctgatg ccagacgcat ccaccgcagt gattgacccg ttcttcgccg actccaccct
    18961 gattatccgt tgcgacatcc ttgaacctgg caccctgcaa ggctatgacc gtgacccgcg
    19021 ctccattgcg aagcgcgccg aagattacct gcgttccact ggcattgccg acaccgtact
    19081 gttcgggcca gaacctgaat tcttcctgtt cgatgacatc cgtttcggat catctatctc
    19141 cggttctcac gttgctatcg acgatatcga aggcgcatgg aactcctcca cccaatatga
    19201 aggtggtaac aaaggtcacc gtccggcagt gaaaggcggt tacttcccgg ttccgccggt
    19261 agactctgct caggatattc gttctgaaat gtgtctggtg atggaacaga tgggcctggt
    19321 ggttgaagcc catcaccacg aagtagcgac tgctggtcag aacgaagtgg ctacccgctt
    19381 caataccatg accaaaaaag ctgacgaaat tcagatctac aaatatgttg tgcacaacgt
    19441 agcgcaccgc ttcggtaaaa ccgcgacctt tatgccaaaa ccgatgttcg gtgataacgg
    19501 ctccggtatg cactgccaca tgtctctgtc taaaaacggc gttaacctgt tcgcaggcga
    19561 caaatacgca ggtctgtctg agcaggcgct gtactacatt ggcggcgtaa tcaaacacgc
    19621 taaagcgatt aacgccctgg caaacccgac caccaactct tataagcgtc tggtcccggg
    19681 ctatgaagca ccggtaatgc tggcttactc tgcgcgtaac cgttctgcgt ctatccgtat
    19741 tccggtggtt tcttctccga aagcacgtcg tatcgaagta cgtttcccgg acccggcggc
    19801 taacccgtac ctgtgctttg ctgccctgct gatggccggt cttgatggta tcaagaacaa
    19861 gatccatccg ggcgaagcca tggacaaaaa cctgtatgac ctgccgccag aagaagcgaa
    19921 agagatccca caggttgcag gctctctgga agaagcactg aacgaactgg atctggaccg
    19981 cgagttcctg aaagccggtg gcgtgttcac tgacgaagca attgatgcgt acatcgctct
    20041 gcgtcgcgaa gaagatgacc gcgtgcgtat gactccgcat ccggtagagt ttgagctgta
    20101 ctacagcgtc taatagttga agttgtacta cccggcgcaa caacgccggg atttagttgc
    20161 cgtggaaact ttcagcccat ctctgcatgg gcttttttct ccgtcaattc tctgatgctt
    20221 cgcgcttttt atccgtaaaa agctataatg cactaaaatg gtgcaacctg ttcaggagac
    20281 tgctttatgg caacaggcac gcagcccgat gctgggcaga tcctcaactc gctgattaac
    20341 agtattttgt taatcgatga caacctggcg atccattacg ccaaccccgc cgcgcaacaa
    20401 ctgctcgccc aaagctcccg caaattgttt ggtacaccgt taccggaact gttgagctac
    20461 ttctcattaa atatcgagct gatgcaagaa agtctggagg cggggcaagg ttttaccgat
    20521 aacgaagtga cgctggtcat cgacgggcgc tcgcatatcc tttctgtgac ggcccagcgt
    20581 atgccggacg gcatgatcct gctggagatg gctccgatgg ataaccagcg ccgcttaagt
    20641 caggaacagc tacagcacgc ccagcaggtt gctgcccgtg atttagtgcg cggcctggca
    20701 catgagatta aaaatccgct tggcggttta cgtggcgcgg cgcagctgct cagcaaagcg
    20761 ttacctgacc catcactact cgaatatacc aaagtgatta tcgaacaggc ggaccggctg
    20821 cgaaatctgg tcgaccgtct gttggggccg cagctgcccg gtacgcgcgt taccgaaagt
    20881 attcacaaag tggctgaacg cgtggtaacg ctggtgtcga tggaactgcc ggacaacgtg
    20941 cggttgattc gtgattacga tcccagcctg ccggaactgg cgcacgaccc ggatcaaatt
    21001 gaacaggtct tgctgaatat tgtgcgcaat gcgctacagg cgctggggcc ggaaggcggt
    21061 gaaatcattc tgcgtacccg caccgcgttt caactgacct tacacggcga gcgctaccgg
    21121 ctggcggcgc ggattgatgt ggaagataac gggccgggca ttccgcctca tttgcaggat
    21181 acgctgtttt acccgatggt cagcggccgc gaaggtggca ccgggcttgg cttatccatc
    21241 gctcgtaatt tgattgatca gcattcaggc aaaattgaat ttaccagttg gccaggacat
    21301 accgagttct cggtttacct gcctatcagg aaataaaggt gacgtttatg caacgaggga
    21361 tagtctgggt agtcgatgac gatagttcca tccgttgggt gcttgaacgt gcgctcgctg
    21421 gagcgggttt aacctgtacg acatttgaga acggcgcgga agtactggag gcgctggcga
    21481 gcaaaacgcc ggatgtgctg ctttcagata tccgtatgcc gggaatggac gggctggcgc
    21541 tgctcaagca gattaaacag cgccatccaa tgcttccggt catcattatg accgcacatt
    21601 ccgatctgga tgctgccgtc agcgcctatc aacaaggggc gtttgattat ctgcccaaac
    21661 cgtttgatat cgacgaagca gtggcgctgg ttgagcgcgc tatcagtcat taccaggaac
    21721 agcagcagcc gcgtaatatt cagcttaacg gcccaacgac cgatatcatc ggcgaagcgc
    21781 cagccatgca ggacgtgttc cgtattatcg gtcggctttc gcgttcttct attagcgtgc
    21841 tgattaacgg cgaatccggc accggtaaag aactggtcgc tcatgccctg catcgccaca
    21901 gtccgcgcgc caaagcgccg tttatcgcgc tgaatatggc agctatccca aaagatttga
    21961 tcgaatcaga actgtttggc cacgagaaag gcgcgtttac tggcgcgaat accattcgtc
    22021 aggggcgttt tgaacaggcc gatggcggta cattattcct cgacgaaatt ggtgatatgc
    22081 cgctggatgt gcagacgcgt ttgctgcgcg tgctggcaga cggtcagttt taccgcgttg
    22141 gcggctatgc gccggtgaaa gtggatgtgc ggattatcgc tgccactcac cagaatctcg
    22201 aacagcgagt gcaggaaggt aagttccgtg aggatctgtt ccaccgcctg aacgttatcc
    22261 gcgttcatct gccgccgctg cgcgaacgtc gggaagatat tccccgtctg gcgcgccatt
    22321 ttttacaggt tgccgcgcac gaactgggcg tagaagcgaa gttactgcat ccggaaaccg
    22381 aagctgctct gacgcgtctg gcgtggccag gcaacgtgcg ccagctggaa aacacctgcc
    22441 gctggctaac ggtgatggcc gccgggcagg aagtgttgat tcaggatttg cccggcgaac
    22501 tgtttgaatc aacggttgcg gagagtactt cgcaaatgca accggacagt tgggcgacgc
    22561 ttcttgcgca gtgggcagac agagcgctgc gttccggtca tcaaaatctg ctttccgaag
    22621 cgcagccaga gctggagcgg acgttactga cgaccgcatt gcgacatacg caggggcata
    22681 aacaggaagc ggcgcggcta ctcggctggg gccgcaacac cctgacgcgt aagttaaaag
    22741 agctggggat ggagtgattc acagcttgtg tgtaaagatt aattattgag cgcaaattgc
    22801 tggtatttta cgctttactg ttccgataag ttcagtatga tcttgcccgg aaaacgggga
    22861 gagtcattat gctggaatca ataattaatc tggtatcgag tggcgcagtt gacagccaca
    22921 caccgcaaac tgccgttgct gccgtgctgt gtgccgcaat gattgggctg tttagttgag
    22981 tttcagattg ctgatgaagt gctctttgtt catgccggat gcggcgtgaa cgccttatcc
    23041 ggcctacaaa agcaatattt tcaacaggtt ttgtaggcct gataagcgta gcgcatcagg
    23101 caatcttgtt ctcagcacat tgatggcgct ttcgttttct actttgtaaa cgaagcgcca
    23161 ttcactattt aaatcacccg tgagaactgc tgcatccgcg ctttctggcg cagataggta
    23221 tcaaagcaca tgcaaatgtt gcggatcagc aagcgacctt tcgccgtcac ctgtattccc
    23281 ttctcatcca catccaccag cccatctttt gctaacgggg cgagcagctt gagatcttcc
    23341 gcaaagtaat cagcgaagtg caaatcccac tgtttctcaa taggggcgta atccagacgg
    23401 aagttgcaga tgagcgactt aatcacatcg cggcgaatac agtcatcacg cgttagcgca
    23461 ataccacgcc acagcgcatt gccttgttca tccacttgct gatagtactg cttcaactct
    23521 ttctggttct gcgcgtagca gtcgccaatc atgctgatgg cggaaacgcc catccccagc
    23581 agatcggtat cgccctgagt ggtgtagccc tggaagttac gatgcagcac gccttcacgc
    23641 tgggccaccg ccagctcgtc atccggacgg gcaaagtgat ccataccgat aaactgatag
    23701 cccgattgcg tcaggaaggc gatggtttcc tgcaggatat cgagtttttg ctgcggactc
    23761 ggcaggtcag catctttgat tttgcgctga gcagcaaaaa tggtcggcag atgcgcgtag
    23821 ttaaagacac tcagacgatc ggggttcagc tccgccacac gtttcagggt aaaggcgaaa
    23881 ctctccggcg tctgtttcgg caggccgtaa atcaggtcga tgttggtgga ggtaaaacca
    23941 atctcacgcg catggttaag cagtgcaaag atgaactctt catcctgctc gcggttaacc
    24001 agacgttgca cttctttgtt gaagtcctgc acgcccatgc tcaggcgatt aaagccttcg
    24061 gcgcgtaaat gatcgagtac atccagttcg atttcccgcg gatcgacttc gatcgaaatc
    24121 tccgcatcgg cattgaactg gaagttttcg cgcagcagct tcatcaggcg gctgatttgc
    24181 gctttattca gatacgtcgg cgttccgccg ccccagtgca attggctgac gtgacgcccg
    24241 gcgaacagcg gtgcacgatg gacgatttct tgctccagcg cgtccagata ctgatcggcc
    24301 ttgtgctgct ggcgagtaac aatcttattg caaccgcaga agtaacaaag cttatggcag
    24361 aacgggatat gtacgtagag agataatgga cgctcaggat agcgcgccac ggcttgtaaa
    24421 aacgcctgtt cgccgaagtc ttctgaaaac tccagcgcgg tcgggtacga ggtgtatcgt
    24481 ggcccggaat agttatattt ctggatcagg gccagatccc agtcgatttg ctgtacagac
    24541 atgctcactc cttccgatgg cgtctctggc ggctacggcg taccggcgga tgcccgtttc
    24601 gggtcatcag ccggttgcgc agccactttt gtcgccgcga caaccgctgt agtttaacga
    24661 ataaccacac cagataacat ataaccggaa gggttatcag cagaacggga aggcccacgg
    24721 cggcaatccg ttagttgccc cgcagcagac gcatcatgtc ttcttgcttc tcgtcttctt
    24781 cctcttcttc gtcatcgtca taagagaggc cgagtttctg catcaactca tcaatacgat
    24841 ccagtttagc atccacccag gattgctctt cggcactcag ggtttcgcct gcttccagac
    24901 gttccagcag cgcatccaga cgctcatccg tttccagtaa ttccaactcc gcctgcggtg
    24961 aaagcatagg tttctcactc ttcggtttgt gctgtttggt gactttttca gtcacgccca
    25021 atggaatagg ggttttactg ccaatacgtg gatcttttgg tgcgttctgg cctttgctgc
    25081 ctgacgtggt gtttccgccc gctgcgcggc tgcccggcgc atgaccacga cgttttttct
    25141 gacgcttgcg gtcacgagct tcctgattca gctcttcgcg cgttttgcga cgcgcttttg
    25201 catgaccttt gctgcgtgat ggtgaagatg gtttcataat ggttcgtctt ttagccgttt
    25261 tattcagtat agatttgcgg cggaatctaa cagaaagcaa gcaaagaaaa aaggcgacag
    25321 attactctgt cgcctttttt cctgactcat aacccttaac gggtcttaca atccttgcag
    25381 gccgccaaca caccccgttt ttccattagc cagaacaatc cgtgttgatt ccatttccgt
    25441 ttaattacgt ctccggacgt ttgtcttcct gacaatcctc tgtcttcgcc tcctggcgct
    25501 cctgaccctc atcctgagtc attgttcctg ttagcgtcct cgctctatgg gtgctacttt
    25561 acgcctttgc tttaaacaaa caatcaaccg ccatcagtat ttgcacattt tcagagtttt
    25621 atataaaagc aattcactga tttatatagc ttatttgaca tgcatcgcag aattttctct
    25681 gctatttcgc ataaagacta tcggcaatgt ctcacaaagc gtatggcttt tacttacaac
    25741 gctcacgccg aaaaatccgc cttttattat ggttcaggta taatcgccac aaagctttga
    25801 ctgacggaga cgaccgcttt gactaatttg aattatcaac agacgcattt tgtgatgagt
    25861 gcgcctgata ttcgccacct accttccgat accggaattg aagtggcttt tgcaggccgt
    25921 tccaacgcag gtaaatccag cgcgctgaac acgctgacta accagaaaag cctggctcgt
    25981 acctcaaaaa ccccagggcg cacccagctt atcaacctgt ttgaagtggc tgacggcaag
    26041 cgtctggttg acttgcctgg gtacggttat gcggaagtcc cggaagagat gaagcgcaaa
    26101 tggcagcgtg cgctcggcga atacctcgaa aaacgtcaga gcctgcaagg tctggtggtg
    26161 ctaatggata ttcgccatcc gctgaaagat ttggatcagc agatgattga gtgggcggta
    26221 gacagcaata tcgccgttct ggtgctgctg accaaagcgg acaaactggc aagcggcgca
    26281 cgtaaagcgc aattgaatat ggtgcgtgaa gctgtactgg cgtttaacgg tgatgtgcag
    26341 gttgaaacgt tttcttcgtt gaagaaacaa ggcgtggaca agctgcggca gaaactggat
    26401 acctggttta gcgagatgca gcctgtagaa gaaacgcagg acggcgaata attttcttgc
    26461 cttaatgctt gtgccggatg tggcgtatcc ggcccgtaaa ttcaatagat tacaattcta
    26521 ccgttggcct gataaacata gcgcatcagg cattacggat cttttctttc gcccaataaa
    26581 aaacgcccca gtcattactg actggggcgg ctaaaatatt cagccaaatc cgattacgtg
    26641 aagtaaaagg tctgaaagat agaacatctt acctctgtac cctacgcgac taactttact
    26701 cttttttgtt cagttcagaa agcacttttt gtaatttttt ttcagttgtt gcataggaat
    26761 ttctaatagc catcacaaaa cgtgacagct tatgttgctt acttacgaaa aaagggcatg
    26821 ttcaggcgaa tcttagtgcg cctgatccca gttttcgcca ctccccactt ccaccagcaa
    26881 cggcacatcc agacgggtac agttttccat cagttgatga atctgcttcg cgacggcatc
    26941 aacatcatct ttatgaactt caaataccag ttcatcgtgt acctgcatga tcatacgtac
    27001 acgcggttgc tcagcctgta accacgcatc aacggcaatc atcgcccgtt tgataatgtc
    27061 ggcggcggtt ccctgcattg gcgcgttaat ggctgcacgt tcagccgctg cacgacgagc
    27121 accattgctg gatttgatat ccggcagata cagacggcgt ccgtccagcg tttcaacgta
    27181 gccctgctct ttcgcctgag cacgggtgcg ttccatatac tccagcacgc cagggtagcg
    27241 ttcgaagtaa aggtccatgt acttctgcgc ttctttacgt ggaatgttca attgccgcgc
    27301 cagaccgaaa gcactcatgc cataaatcag accaaagttg atcgctttcg cgctacggcg
    27361 ttgctcgctg gtgacggttt ccagtggcaa accaaacact tctgccgccg ttgcccggtg
    27421 gatatctttt ccttccgcga atgcggtcag caagcctttg tcacgcgaaa gatgcgccat
    27481 aatgcgcagt tcaatctgcg agtagtccgc tgagacaatc acataatcct ctggcgcaat
    27541 aaacgcctgg cggatacgac gaccttcttc gttacgcacc ggaatgtttt gcaggttagg
    27601 atcggttgac gataaacgtc ccgttgcagt tactgcctgg tgataagagg tatgcacacg
    27661 cccggttttc gggttgatca tcagcggcag cttgtcggtg taggtcgatt tcagcttcgc
    27721 cagaccacga tactccagaa tcacttttgg caacggatag tccagcgcca gttcttccag
    27781 tacctcttcc gacgttgacg gcgcgccacc cggcgttttc ttcagcggtt taatgccctg
    27841 tttttcaaag agaatggttt gtaactgctt ggtggaagaa aggttaaatt cctcacctgc
    27901 aatttcatgc gctttctttt ccagctcagc cagacgaagg gtgagctctt cagaatgatt
    27961 gtgcagcact ttcggatcga tcttcacacc gttacgttca atgcgtgaaa gcaccggcac
    28021 cagcggcatt tcgatattct cgaagacgtt caacggccct ttgtgttttt gcagatccgg
    28081 ccacattttc agatgcaact gcaaggtgac atctgcatct tcggcggcgt aacgtccggc
    28141 ttcttcgagg gcaatctggt taaaggtcag ttgatttttg cctttaccag caatctcttc
    28201 aaaagtgatg gttttgtgct tcaaccaacg ttccgcgagg ctgtccatat cgtgacgccc
    28261 ggcaacgcta ttgagaatgt aggactccag catggtatca aacgcaatcc cacgcagttc
    28321 aatgccgtag ttcgccagaa taccgcgatc gtatttcagg ttttgcccga ccttcagcgc
    28381 cttttcatct tccagcagcg gttttagcaa ctcgagtgca cgctcgcgag agatttgatc
    28441 gggcgcatca agataatcat gagcaaccgg aatatatgcc gctacgcctg gctcgatagc
    28501 aaaagaaagc ccgaccaggt tagcagagat gttatcaagg ctgtcggttt cggtatcaaa
    28561 tgcaaatacc ggcgcttttt ccagcttcgc aatccacgct ttcagtgttt cttcatcaag
    28621 gatggtgacg tagttgtcat aagaaatcac cgttgccgtc acttctggtg cttcgtctgc
    28681 aacactggtt tcctgtggct tcgcggctgg ttttgcccct ttggcctgta accatttgcc
    28741 cgcttcgaca tcagcagtcc agcgtttgaa ctcatacttt ttgaacagcc ccaacaactc
    28801 ttccgctgcc ggttgctgca cttccagttg ttcacaggtc agctccagtt caacgtcggt
    28861 tttaatcgtc gccagctggt atgagagata agcaacttct ttgttttgct cgagcttcgc
    28921 tgccattgtt ttcgcgccac ggaagctcaa cccagcaatt ttttctggct cggcatacag
    28981 cgtatccagt ccgccaagac cttgcagcaa tgcctgcgcg gttttttcac cgacgcccgg
    29041 tacgccagga atgttatcag aggagtcacc catcagcgcc aggaaatcga tgatcagttc
    29101 tggcggcacg ccgtacttat tcaccacctc ttccggtccg aggatggtat tcgtcatggt
    29161 attgataagc gtaatatttg gcgtcaccag ctgcgccata tctttatcgc cagtgctgat
    29221 cagcaccgga cgcccggctt tttcggcttc gcgcgccaga gtaccgataa cgtcgtccgc
    29281 ttctacgcca gaaaccgcca gcagcggcag tcccatcgct ttaaccatcg cgtgcaaggg
    29341 ttcgatttgt gcacgcagat cgtccggcat tggcgggcga tgtgatttgt aatgttcaaa
    29401 cagttcatca cgaaaggttt ttcccttggc gtcaaagacc accgctgcat gcgtcggttt
    29461 atattgcatg atcagactgc gcagcatgtt gaggacacca tacatcgcac cggtcggctc
    29521 gcctgcgctg ttagtcagcg ggggaaacgc gtgatatgcg cgataaagat aagatgaacc
    29581 atctacaagg ataagtggat tttgggggat ctgaaccata atgtccgtgc ctgtttatca
    29641 gattatgggt aaaggatgcc acagacagga taaaaacatg agtttttcac cgcttttcgt
    29701 gaaaatcttt tgtagatctt ctggatcgct cgcaaatcgt catgtggata actttgtgca
    29761 taaattattt acatcgcttt taatttatta tcatgctttt tacagcatga tgtttgtctt
    29821 tgtttttcat gcagttaaaa ataaagccat ccctaata
//
LOCUS       DAPHBJ010000036        29090 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.036, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000036 DAPHBJ010000000
VERSION     DAPHBJ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.036"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            99..1682
                     /locus_tag="RHZ76_001473"
     CDS             99..1682
                     /locus_tag="RHZ76_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDV2812133.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(1975..3828)
                     /gene="asmA"
                     /locus_tag="RHZ76_001474"
     CDS             complement(1975..3828)
                     /gene="asmA"
                     /locus_tag="RHZ76_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HDV2812134.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(3850..4431)
                     /gene="dcd"
                     /locus_tag="RHZ76_001475"
     CDS             complement(3850..4431)
                     /gene="dcd"
                     /locus_tag="RHZ76_001475"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HDV2812135.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4523..5164)
                     /gene="udk"
                     /locus_tag="RHZ76_001476"
     CDS             complement(4523..5164)
                     /gene="udk"
                     /locus_tag="RHZ76_001476"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDV2812136.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5482..8799
                     /locus_tag="RHZ76_001477"
     CDS             5482..8799
                     /locus_tag="RHZ76_001477"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDV2812137.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETHFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMLAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGEVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(8908..9756)
                     /gene="alkA"
                     /locus_tag="RHZ76_001478"
     CDS             complement(8908..9756)
                     /gene="alkA"
                     /locus_tag="RHZ76_001478"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HDV2812138.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGRLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            9890..11242
                     /gene="yegD"
                     /locus_tag="RHZ76_001479"
     CDS             9890..11242
                     /gene="yegD"
                     /locus_tag="RHZ76_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV2812139.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11255..13195)
                     /gene="yegI"
                     /locus_tag="RHZ76_001480"
     CDS             complement(11255..13195)
                     /gene="yegI"
                     /locus_tag="RHZ76_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HDV2812140.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVDSVAKIYHTP
                     PPASKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLAMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDLLRQQLKKCTVSAMHVYSAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRTMLEKMKDEMLGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13192..13953)
                     /locus_tag="RHZ76_001481"
     CDS             complement(13192..13953)
                     /locus_tag="RHZ76_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HDV2812141.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAKLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGRGLELPLTPMVGEYANMTHFI
                     SDEDAVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFIGLAAATQE
                     QLDLLPKLLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(13950..14609)
                     /locus_tag="RHZ76_001482"
     CDS             complement(13950..14609)
                     /locus_tag="RHZ76_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDV2812142.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLSLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(14830..14889)
                     /gene="ibsA"
                     /locus_tag="RHZ76_001483"
     CDS             complement(14830..14889)
                     /gene="ibsA"
                     /locus_tag="RHZ76_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HDV2812143.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15162..15218)
                     /locus_tag="RHZ76_001484"
     CDS             complement(15162..15218)
                     /locus_tag="RHZ76_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDV2812144.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            15497..16744
                     /gene="mdtA"
                     /locus_tag="RHZ76_001485"
     CDS             15497..16744
                     /gene="mdtA"
                     /locus_tag="RHZ76_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HDV2812145.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLIALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            16744..19866
                     /gene="mdtB"
                     /locus_tag="RHZ76_001486"
     CDS             16744..19866
                     /gene="mdtB"
                     /locus_tag="RHZ76_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HDV2812146.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENAPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALLIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            19867..22944
                     /gene="mdtC"
                     /locus_tag="RHZ76_001487"
     CDS             19867..22944
                     /gene="mdtC"
                     /locus_tag="RHZ76_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HDV2812147.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAVSNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            22945..24360
                     /locus_tag="RHZ76_001488"
     CDS             22945..24360
                     /locus_tag="RHZ76_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2812148.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLTIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            24357..25760
                     /gene="baeS"
                     /locus_tag="RHZ76_001489"
     CDS             24357..25760
                     /gene="baeS"
                     /locus_tag="RHZ76_001489"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HDV2812149.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLQISAGQRDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            25757..26479
                     /gene="baeR"
                     /locus_tag="RHZ76_001490"
     CDS             25757..26479
                     /gene="baeR"
                     /locus_tag="RHZ76_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HDV2812150.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            26670..27002
                     /locus_tag="RHZ76_001491"
     CDS             26670..27002
                     /locus_tag="RHZ76_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HDV2812151.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27149..28510
                     /gene="yegQ"
                     /locus_tag="RHZ76_001492"
     CDS             27149..28510
                     /gene="yegQ"
                     /locus_tag="RHZ76_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HDV2812152.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNIYFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(28840..>29090)
                     /locus_tag="RHZ76_001493"
     CDS             complement(28840..>29090)
                     /locus_tag="RHZ76_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812153.1"
                     /translation="ETSIGKFGLAYKSNIQRKLDNQYYTEAEASLARGRISGAENIVK
                     NDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFKCGE"
BASE COUNT         6668 a         7828 c         7706 g         6888 t
ORIGIN      
        1 tatgagataa atagtgctgc aacattgcat ttttgccccg atttatccat gatcgaattg
       61 tgacatttgt catacaacga ataggttttg tacttactat ggaatggatt gccgatccgt
      121 ctatctgggc cgggttaatc acgctgattg tgatcgaact ggtcctcggc attgataacc
      181 tggtctttat tgccatcctc gccgaaaaac taccgccgaa gcagcgcgac cgcgcacggg
      241 ttaccgggct gctgctggcg atgttaatgc gcctgttact gctggcgtca atctcctggc
      301 tggtcaccct gactcaaccg ctgttcagct tccgctcgtt tacctttagc gcccgcgact
      361 taatcatgct gtttggtggt ttcttcctgt tgttcaaagc cacgatggag ctgaacgaac
      421 ggctggaagg gaaagacagc aataatccca cacaacgcaa aggtgcgaag ttctgggggg
      481 tggtgacaca aattgtggta ctggacgcca tcttctcact cgactcggtg attaccgccg
      541 tcgggatggt cgaccattta ctggtcatga tggccgccgt ggttatcgct atcagcctga
      601 tgttgatggc cagcaagccg ttaacccagt tcgttaatag ccacccgacc atcgttattc
      661 tctgcttaag cttcctgttg atgattggct ttagcctggt ggcagaaggt ttcggcttcg
      721 tcattccgaa aggctacctg tacgctgcca ttggtttctc ggtgatgatc gaggcactca
      781 atcagttggc tatctttaac cggcgacgtt ttctttccgc taatcagacg ctacgccagc
      841 ggactaccga agcggtaatg cgcctgctta gcgggcaaaa agaagatgcg gaactggacg
      901 ccgaaaccgc gtccatgctg gtggatcatg gaaaccagca gatctttaat ccgcaggaac
      961 ggcggatgat tgagcgggta cttaatctta accagcgtac cgtcagcagc attatgacgt
     1021 cgcgccacga tattgagcat atcgatctca acgcgccgga agaagaaatc cgccagttgc
     1081 tggagcgaaa tcagcatacg cggctggtgg ttaccgacgg tgatgacgca gaagatttgc
     1141 tcggtgttgt tcacgttatc gacctgctac aacagtctct gcgcggcgaa ccgctcaacc
     1201 tgcgggtgtt gattcgccag ccgctggtgt tcccggaaac cttgccgttg ttacctgccc
     1261 tggagcagtt ccgtaatgcc cgcacgcact ttgcttttgt agtggatgag tttggctcgg
     1321 tggaagggat tgtgacatta agtgacgtca ctgaaaccat tgccggtaac ttaccgaacg
     1381 aagtggaaga gatcgacgcc cgccatgata ttcagaaaaa tgcggacggt tcctggacgg
     1441 cgaatggtca tatgccgctg gaggatctgg tgcaatatgt gccgctaccg ctggatgaaa
     1501 aacgtgaata tcacaccatt gccgggctgt tgatggaata tttgcagcgt attccaaaac
     1561 ctggcgaaga agttcaggtg ggggattatt tgcttaaaac gttgcaggta gaaagccatc
     1621 gcgtgcagaa ggtgcagatt ataccgctgc gtaaggatgg cgagatggag tacgaggtgt
     1681 gatggtatcg gtcattttac ccgatgctgc gtaacgcctt ataacttcag caatgaagcg
     1741 ttgtctgtgg taaacggaca tcacagcggg ggtgtgggcc cgggttgcca gggtggtgga
     1801 gattgagcca ccctggcacg ttcaccggct tgagcgttcc atagtagcga ggaacatcaa
     1861 gtgaacggaa cagccttcga tcttacatat tcagaatata atgatgccgc cacgaacgcc
     1921 tgatgcgacg ctttacgcgt cttatcaggc ctacaggact ggtcacctgg taacctacat
     1981 cttctccagc aacttcttca catctttgcc attgcgggaa tctttattcc gctcggccca
     2041 gtcattcagg cgacgtttcg cttcgtcctg tagatgtttg cgcagtaact gatccacttg
     2101 cagactgtaa ttgagttgct gccagttgcc ataaacccgc agcggtactg gcgtttcttt
     2161 cagaaaatca atcagtttac tttccccgtt ccagccaccc acgacccgaa tatcaaactg
     2221 ggtgtcgcag gtttgatctg ccagattcaa catgccttcc cctgtcagcg ccagcacagg
     2281 cgattgacct tgcatgtcgt ttaacgtcac gacgccatcc ttcaacgtca aatcggtggt
     2341 aaagcggtca agccgcgtca cgttatcaaa gttttcagcg gccttcacat caccgccatt
     2401 acgctctacc gcttgctgaa tcatctgctg gaagttcatc ccttccatgc gcgtgtcggt
     2461 catttcgaca tgtgcctgcc cttgccagtt gtggcggaat gcgtcggcat ctatgtcagc
     2521 accggagaaa tcaccagcca gtgacatttt tccggtcaac gaaatcggat agttaaacgc
     2581 cttcagaatg gtaccaatct caacgttttc cagccgtggc tggaagttta tccgcggatt
     2641 tattgatgtc gcgtccagcg tgcccggcag tgaaacctgt ccaccgttaa gcttgccctg
     2701 cagttgagta atttccagca aacccgactt gttggtcatt tgcgtggcaa catctgtaaa
     2761 attcattccg cgccagcgca cgttactggc ctgcaacaaa atatcagccg taaagccttg
     2821 cagtccctga taggccggtt catcaatacg cgaagaaata accgggcgcg gcaacgttga
     2881 ttggctctgc ccctgctgcg cggcaccgtt ttcaccattc gcggtttcat taagcgggat
     2941 gaggttgtca agattcagtt gcgggaattg cagtctcagc tgccattcag gtttctctgt
     3001 cagcgtgacc tgtgcttgcc cgctcagcgt actgtcattg gcggtcaagc tgatttggtt
     3061 aaatgaaagg cgtttatgtg actcctgcca ctgggcctgg aaactcccct gcccctgaat
     3121 accttgtttt ggtaaatcgg caccctgcaa ctgccagtta atttgttcaa tagcagccgt
     3181 taaatcatgc ggataatcag aagcatctac cgtaccgtta agggatatcg tgagatcgcg
     3241 ctgatcgcga ttaacccgcc cggagaactc aaatgaacca cgatgttggg gatcttgttc
     3301 catttgcagg cgaatattgc ggattgtcac ctgctcgtca tcttcatgct ggaacaccag
     3361 cacgctgtcc gccaccttaa gactggatat atcaaacgac catccgcgat cgtctgacag
     3421 atccggcaag gtattgtcgc gcggtgcaac cggagcgtct tcactgcgca ccgcttccgt
     3481 ctgcggcgtc agttggatca ctgccccttt tagcatcacc tgcttaacgc tcagttgatg
     3541 actcagtagt ggtaaaagcg ccacgtccag acgcatgttg tcggcgcgaa ccagtggctg
     3601 gctcgcgccc tgggcggtga gagacattcg cccggagagg atactaagct gcggccagac
     3661 gtgccaacgc agtggcccgt cgagctgcaa ttgataaccg ctacgtgcag caacttgctt
     3721 gaccatatag tcgcggaaat cattcggatt caccagcaac actaacgcag ataacccggc
     3781 caccagcacg accaggagta tcatcagcgt cgtcagaaat cgtctcatgg tatcctcaat
     3841 gggcctgaat tagtctttat cgattcggct ggctaccgcg ccctgctggt tgcgatattt
     3901 cgcatcttca cggcggttgt aaggtcgcgc cgccgggccg gaaagcggct caaagctcag
     3961 cgcaccaatt aacatgcccg gacgcagcgc cagcggcagc ttaccggagt tgtagaactc
     4021 cagcacaatg caaccagacc agcccggatc gatacggtgc gcggtgacgt gcaccatcag
     4081 ccccagacgc gccaaagagg aacgcccgtc cagccagccc accagatcgg ctggcagcgt
     4141 caccgactcc agcgtcaccg ccagcgccag ctctcctggg tgaagataga acgcctcgcc
     4201 ctcgtcgaga acgatctcat cgctcatcac gcggtcaagc gcggcgctca cttcatcttt
     4261 gggaccgctc agatcgataa acgctgccgt gtgaccacgg aaggtacgaa atttattgcc
     4321 caggcgtaca tccaccgtcg cgccgttaat acgctccact ggcggacgtg ggttgatcga
     4381 caaacggcct tcatcaagcc aggcttcaat atctcggtca cacagacgca tggcattttc
     4441 tcctttcgcg catcactccc ttaacgccaa ttacgtcaag ggcataccag gttatcactg
     4501 aacggtacac aatttatcaa gcttattcaa agaactgact tattttcgct ttcaatatat
     4561 cgatcgcaat gcggtttttc ccgccgcgcg gcacgataat gtccgcatat tgtttagaag
     4621 gctcaatgaa ttgcaggaac atcgggcgca cggttttttg atattgcgcc atcacggaat
     4681 ccattgaacg cccacgctcg ttaacgtcac gcttgatgcg gcgcatcagg cagatatcca
     4741 gcggggtatc aacgaaaatg gagaagttaa gttcgtcacg caaacgcgca tccgtcagca
     4801 acaaaatgcc ttcgagaatg atgaccttct tcggctcaac cgtcaccgtt tctttcatac
     4861 gcgtatgttc aacatagctg taaaccggca ggtcaattgc cgagccgcgt ttcaacgctt
     4921 gtaaatgctc aagcagcaga ctgtgatcca tcgcgctggg atggtcgtag ttggtcttaa
     4981 cgcgttcttc catcgacaga tggctttgat ctttgtaata gcagtcttcg ggaattacgc
     5041 cgatgtgttc atcaccgact tgctcacgca attcacgata aagggtactg gcaataagac
     5101 tcttgccgga agccgatgcg ccagcgatac cgataatgac gcactgatga gactgatcag
     5161 tcatatattt agcgacctga ttaacctgga tgttaggaag ggggcgacga agcgccaaac
     5221 gcggcaatta tagggatttc atccgtctga taccagtcga atagcgttgc tgcgcgctca
     5281 gagttaattg ttgacaaaga attcccgggg gcaaattacg ttgatcagtt ttatgtaagg
     5341 taaaaaatgt tatacgcagt tgcgcaaatt atccgccttt acgtcacttt atgagcaatt
     5401 cgcatataaa atgtaaaact tttgtactag cataaacaca gaaacgaata ctggcgacca
     5461 ggtcttgcgg ataaagcggt aatgagcaaa caatcacagc atgtattaat tgccctgccc
     5521 cacccgctgc ttcacctggt cagtttaggt ttagtctcgt ttatctttac ccttttctcg
     5581 cttgagcttt cgcagtttgg cacccaactc gccccactgt ggttcccgac gtccatcatg
     5641 atggtggcgt tttatcgcca tgccgggcgc atgtggccgg gaattgcgct aagctgttcg
     5701 ctgggaaata tcgccgcatc catcctgctt ttttccacca gctcgctgaa catgacctgg
     5761 acgaccatca atattgttga agccgtggtc ggggcagtgc tgctacgtaa attgctgccg
     5821 tggtataacc ccttgcaaaa tctggctgac tggctgcgtc tggcactcgg cagcgccatt
     5881 gttccacctc tgttgggggg tgttctggtt gtcctgctga cgcccggaga cgatcctctc
     5941 agggcatttt tgatatgggt actgtcagaa tccatcggcg ctctggcact ggtgccgctg
     6001 ggattgttat ttaaaccaca ctatctgcta cgccatcgca acccacggtt gctttttgag
     6061 tcgctgctca cgttagccat cacactgacg ttaagctggc tttcgatgct gtatctgccg
     6121 tggcctttta ctttcattat tgtgctgttg atgtggagcg ccgtgcgtct gccacgaatg
     6181 gaagcctttt tgatcttcct taccacggtg atgatggtgt cgctgatgat ggccgcggat
     6241 ccctccctgc ttgctacgcc gcgtacgtac ctgatgagcc atatgccgtg gctaccgttt
     6301 ttgctgatcc tgctgcccgc caacatcatg accatggtga tgtatgcctt tcgtgcggaa
     6361 cgcaaacaca tttccgaaag cgaaacccac tttcggaacg cgatggaata ttccgctatc
     6421 ggtatggcgt tagtgggcac cgagggacaa tggctgcaaa ccaacaaagc gctctgccag
     6481 tttctcgggt acagtcagga agagctgcgc ggactcacct ttcagcaact gacctggccg
     6541 gaggatctca ataaagatct ccaacaggtt gaaaagctga taagcggtga aataaacacc
     6601 tattcaatgg aaaaacgcta ctacaaccgc aatggcgatg ttgtctgggc gttgcttgcc
     6661 gtctcactgg tgcgccacac ggatggcacg ccgctctatt ttatcgctca gattgaagac
     6721 attaacgagc taaaacgcac cgaacaggtc aatcagcaac tgatggagcg catcactctg
     6781 gctaacgaag cgggcgggat tggcatctgg gagtgggagt tgaagccgaa tatttttagc
     6841 tgggataagc ggatgttcga gctgtatgaa attcctccac atatcaaacc gaactggcag
     6901 gtgtggtacg agtgcgtgct gccggaagat cgccagcatg ccgaaaaagt gattcgtgat
     6961 tcgttgcaat cacgctcgcc ctttaaactg gaatttcgca ttaccgtaaa agacggtatt
     7021 cgccatatcc gcgccctcgc caaccgggta ctgaataaag aaggcgaagt cgaacgtctc
     7081 ctcggcatca atatggatat gaccgaagtg aaacagctta acgaggcatt gtttcaggaa
     7141 aaagagcgcc tgcacattac gcttgattcc atcggtgaag ccgtggtctg tattgatatg
     7201 gcgatgaaaa ttacctttat gaatccagtg gcggagaaga tgagcggctg gacgcaggaa
     7261 gaagcgttag gtgttccgct cctgacggtg ttgcatatta cttttggcga caacggacca
     7321 ttaatggaga acatttacag tgccgacacc tcacgttccg cgatcgaaca agatgtggtg
     7381 ttgcactgtc ggagcggcgg cagttacgac gtgcattaca gtattacgcc gttaagtact
     7441 ctggacggca gcaatattgg ttcggttctg gtgattcagg acgttaccga atcacgcaaa
     7501 atgctgcgcc agctgagcta cagcgcctcc catgatgcac tgacgcatct cgccaaccgc
     7561 gccagttttg agaaacaact gcgtatcctg ctgcaaacgg taaacagtac gcatcagcga
     7621 catgcactgg tgtttatcga tcttgatcgc tttaaagcgg tgaatgacag cgccgggcat
     7681 gcggcgggcg acgctttgct gcgcgaactg gcgtcgttga tgctgagtat gctgcgctcc
     7741 agtgacgtgc tggcgcggct cggcggtgat gaatttggtc tgctgctgcc agactgcaat
     7801 gttgaaagcg ctcgttttat cgctacacgt attatcagtg ctgtgaatga ctatcacttt
     7861 atatgggaag gccgggtaca tcgggtaggt gccagcgccg ggattacctt gatcgatgac
     7921 aacaatcatc aggcggcaga agtgatgtcg caggctgata tcgcctgtta tgcctccaaa
     7981 aatggtggcc ggggccgggt gacggtttac gaaccgcagc aagctgccgc acatagcgaa
     8041 cgggcggcga tgtcgcttga tgaacagtgg cggatgatta aagagaatca gttgatgatg
     8101 ctcgcccacg gtgtcgcttc gccacggatc ccggaagcgc gtaatttgtg gctgatttca
     8161 cttaagctct ggagttgcga aggcgagatt attgatgaac aaacatttcg tcgtagcttc
     8221 agcgatccgg cgcttagcca tgctcttgac cgccgggtat tccacgaatt tttccagcag
     8281 gccgcaaaag cggttgccag taaaggcata agcatcgccc tccccctttc cgttgccggt
     8341 ttgagtagcg ccacgctggt gaatgatctg cttgagcagc tggaaaatag ccctctacca
     8401 ccacggttat tacatctgat tattccggct gaagcgattt tagatcacgc agaaagcgtg
     8461 caaaaactgc ggctggcggg atgtcggata gtgctcagcc aggtgggccg cgatctgcaa
     8521 atcttcaact cgctgaaagc gaatatggca gattacctgc tacttgatgg tgagttatgc
     8581 gccaacgtgc agggtaattt gatggatgag atgctgatta cgattattca ggggcacgct
     8641 cagcgactcg ggatgaaaac catcgccggg ccagtcgttt tacccttagt gatggatacg
     8701 ctttctggca tcggcgtcga tctgatttat ggtgaggtga ttgccgatgc ccaaccgctg
     8761 gatttgctgg tgaatagtag ttatttcgcg attaactgag gcgtgcttcc ccatcgcctg
     8821 atgcgacgct aacgcgtctt atcatgccta caaatcgctc attccccagg ccggataagg
     8881 cgctcgcacc gcatccggcg accaacgtca tgcttcgtct ggttgccagc cttccgtata
     8941 ccagatatgc aacagcgcat aagaacgcca gggcttccag cgctcggcat aacggcggat
     9001 ttgcgccggt gtcattcccg gaaatcgctg tttaatcaga taatcatccg gcaggaaaac
     9061 atctttcgcc tgccagccac gcaaagcaaa ataattcgcc gtccagcgcc cgatacccgg
     9121 aaaagtttgc agcgttttca tcgcctgctc cacatcgccc ggtattgtca ttggtaaggt
     9181 gccctccagc gccgcatttg ccagatgaat cagcgcctct gcccgtttca acggcatacc
     9241 taacgctttt aatgcctgcg ggtctgctgc tgccagccgc tgaggcgtgg ggaagcagat
     9301 atactccgga aaatcatcca gccgttcgcc ataaagctgt accactttgg cagtcaattt
     9361 tgccgccatc gccacgctca ccagttggcc taaaatcgcc cgcacgccct gctcaaaagc
     9421 atcaacactg ccgggtaaac gcaatccggg ccgcgccgcg cctaacctgc ccaacgcacc
     9481 gttaacaatt tgtgggttac attgcagatc aaacaggcgg ctcattttcg ccagacactc
     9541 tgcggcaaca ggttctaaac ctgcacttaa atttatgtgc agagtatggc gggctatatc
     9601 cggaatagca gtcaccacgc cgcgatattc gcccaccgcc agactacggg cataataact
     9661 gtccgcgacc gtttccacac cgctcaccgc acgggcggcg agaaatccca acatccacga
     9721 ccagtcatac ggcggctgcc agttcagggt atacatcgca tctccttatt catccgcttt
     9781 cagcataaac gttattcaga cgctgcgctt tgctttcata ttccggttgt cgcgacggca
     9841 acatttcgct aaagtcacgc cccttcttca ccggcatggg gattatttcg tgtttattgg
     9901 ttttgattac ggtacagcaa actgttcagt ggcggtcatg cgtgacggta aaccgcactt
     9961 gctaaaaatg gaaaacgaca gcacgctgct gccttcaatg ctttgcgcgc caacgcgtga
    10021 agcggtaagc gaatggctgt accgccatca tgatgttccg gcagacgacg atgaaacgca
    10081 ggcgctgttg cgtcgggcga ttcgttataa ccgcgaagaa gatatcgatg ttacggcgaa
    10141 aagcgtgcag ttcggtcttt cctcactggc acagtacatt gatgatccag aagaagtgtg
    10201 gtttgtgaaa tcaccaaaat cgttcctcgg tgccagcggc ttaaaaccgc agcaggtagc
    10261 gctgtttgag gatctggtct gcgcaatgat gttgcacatt cgccagcagg cgcaggcgca
    10321 gctgccagaa gcgattactc aggcggtgat tggtcgcccg atcaacttcc aggggctggg
    10381 cggcgatgaa gcaaacgccc aggcgcaagg gattctggaa cgcgcggcga agcgtgccgg
    10441 attcagggac gtggtattcc agtacgagcc ggtcgcggct gggctggatt acgaagccac
    10501 cttgcaggaa gaaaaacggg tgctggtggt ggatatcggc ggtggtacga ctgactgttc
    10561 attgctgctg atgggaccgc agtggcgttc gcgtctcgat cgtgaagcca gcctgctggg
    10621 tcacagtggt tgtcgtattg gcggtaacga tctggatatc gccctggcgt ttaaaaacct
    10681 gatgccactg ctgggtatgg gtggcgaaac cgaaaaaggc atcgccctgc cgatcctgcc
    10741 gtggtggaat gcggttgcca tcaacgacgt acctgcgcag agtgatttct acagtagtgc
    10801 caacggtcgt ctgcttaacg atctggtacg cgatgcccgc gaaccggaaa aagtggccct
    10861 gttacagaaa gtctggcgtc agcgtttaag ctatcgcctg gtgcgtagcg cagaagagtg
    10921 caaaattgct ctttcaagcg tagcggaaac ccgcgcctca ctgccgttta tcagcaatga
    10981 actggctacg ctgattagcc agcgagggct ggaaagcgcc ctcagccagc cactggcgcg
    11041 gattctggaa caggtgcaac tggccctgga taacgcccag gaaaaaccgg acgttatcta
    11101 cctgaccggc ggtagcgccc gttctccgct gattaaaaaa gcgctggcag aacagttgcc
    11161 gggcattccg attgcaggcg gcgatgactt tggctccgtc accgccgggc tggcacgctg
    11221 ggcggaagtg gtgtttcgtt aattgaaaaa tggctcagca gcggcttaaa tctgcctgcg
    11281 cctgagccag tttttctgct gcctgacgta acggttccat caacggcatg gtccgtgctg
    11341 gagcatgaag gcggaatcgc tgcaactctg tcgcgccgac tgtcaacgcc gattccagcc
    11401 gatgtcgttt ggcggtcatt ttcgccatta ccgcttgtct gtctgctggc gttatcgctt
    11461 cattcggcct gaacacaaaa cggcgttcac agctcgcttt ccagtcgata accgcctggg
    11521 tcagatggtc gccaaaccct ttcacttgct taacgccacg acgggtaaca tctgcggctg
    11581 tttcaatacc aaaagaccgt aacgccgctt tacgcgcagg gccaacgccg ggaatagagg
    11641 caacatcaat aaaaaatccc tccagaaact tctgcttctg ccgttccctt gcggtgtcgt
    11701 gaagtgctgc cagagcacgt ttttcttctt ccggtaaccc gagcatttcg tccttcattt
    11761 tttcgagcat cgtccgtttg gcgataaaac cttccagccc gcccaactgt tggatctggc
    11821 tgactaaatg gtcatagtcc attttcgcgc gattaaatgc ctcacggcgt tgctgaactt
    11881 cagctttgta cgctttgctt gtcagactgc caataatcca gatagccgac agcacaggaa
    11941 ccaaaataat ataacgcggt tctgcctgaa ggccgcaaag caacagcgat aacgctgaca
    12001 gtgcgatctc aatcagaatg atgtattcgc gccgtaacag gcctaaagga agcggcctgc
    12061 cagtcggttg gaaatgatcg ggtaatggca attgcaatgc tggcggtgct actgacgcca
    12121 tcaccatcgc ccagactttc gccagcacaa aatcaccgct ggtggtaatg acctcttcgc
    12181 cgagatcaat aaaatagata acaccttgat tatccagcgc acaccacggg cagtcagtca
    12241 aatgagcgga gtaaacatgc attgccgaaa cggtacattt ctttaactgt tggcgtagta
    12301 aatcaagtgc cgctacccac gcttttgccg tcggacgccc ggttgccacg ccactttccg
    12361 taaacgcctg ctgaaacatg gcttcaacat cgcccggtaa catcgccagc ggaatagatc
    12421 gtggcggcgg ttttaaacca cgtcgctgat tatctgacgc gtaggcataa cggaaatggg
    12481 caatatccgt ctccagcgca ttacccgcat cagagataag cggcacaccg gaataaggat
    12541 gccgaccacc aaacaagacg tgaaaaatca acaaggcaag gccaaaatta tcgtgatttt
    12601 ctgtacgttc aaagccgaca aatgacgaca aggtttgtag ctctggcggc gtaaaatgcg
    12661 acacgccgac ttcgcataaa tgcagtgtgc cattggcgtt aatctgaaag gagtcgctat
    12721 caatcaacac cactttgctg tcgcgaccta ccataaaact gttctggttt acgtcaccca
    12781 cgacgtgccc gtgctcgtga accgtagcaa aagatgaagc aatattgcgc gcaacataga
    12841 gtagaaaatc ccacgcacaa tgagggtaac tctggcgacg atgtgccggg ctatagatca
    12901 tatgaatcgg ttctttacca gaaacttttg gcatcataaa accgataact tttccgcctc
    12961 gtccaccgtg aagcgttgcc tgcggccagg cgacataatt caacaactgc gcgtcagctg
    13021 tcgcagccat aaaggcaagt ttgtcctgtt ttgaggcggg tggcggcgtg tgataaatct
    13081 tggcgacgct atcgacaaac tcctcgatat cataaaccgc gccttcacca cctttgccca
    13141 gttcacggcc tggcgtaacg cactcaccag tagcagtata taaagtgggt ttcatggtaa
    13201 ccacagcgcc agtgccaacg ttttatcatc atcggtacgc tcgtttaccg ccgggctgga
    13261 caaaaactgc tttaacaatt taggtaataa atcgagttgt tcctgcgttg ctgccgccag
    13321 tccaatgaaa aacggggtaa agaaaggcac atgcggagaa ttatccagca tatttaacgc
    13381 cagtcgctga ataccatccg taaatgctgc aactttatgc gcacgctcag tgctggtgaa
    13441 agtttccagt ctggaaacag catcttcatc gctaatgaaa tgcgtcatat tggcatattc
    13501 acccaccatc ggcgtgagcg gcagttccag accacgacca aaatccacca ctacaccgcc
    13561 gtcgccaatt tgcataatca atgtgccatt agccgatgaa atcaatccca gaaacgtgca
    13621 ggcaaaatcg cgaactgcta atgctttggc ttctgcctcg gcaaaaagtc gttgacgaat
    13681 ggtcagtacc atatttgtgg cgagaacatc attaagcccc aattcaccgt cctgtacttt
    13741 ttgcaccata aacgccatcg cttcattgac ggcgagcttc gcgccttctc ctccctgcga
    13801 aacgctacct gcgccatcag caaggaacat caccagtaat ggctgctggt catttagcca
    13861 ggcaacctgc atctggcagg catcctgaca aggtaaatca gcgctaatgt gcgacgttcc
    13921 aacagcggat gcataaacca gacgccagct cacactgacg tccagccttt tggcgcttcc
    13981 agcacaactt ccgttcccgg cgtggagcgg gaaaccgaac gcagcgaact ggataaccag
    14041 ctaaacagtt cacggaattg taatccctgc agagacaaag gctgacggac gctgatttgg
    14101 gccagcgtct tcatatccgc cccctgaaca ccgatggaaa agaaagcaaa ccttttatct
    14161 tcttcccccc gaaacacttt gttggctgca gcctgccact catcggttgg tgcaccatcg
    14221 gtaatcagga aaatccacgg gcgataatac gaaataccat ttgcacgata ctcacgtttc
    14281 cgctcctcta ccatatccag ggctttggta atggccgcac ccattggtgt atcgccctgg
    14341 gcaaacagga tgggcggaaa aaaattagcg gcgctggtaa agggttgttc cacatgcacc
    14401 gggccgaacg tcacaatgcc gagttccact ctttttaaag ccagcggatc agcaagcagt
    14461 tcatcacgaa aggtaaccaa tccggcgtta agttcattaa taggtctgcc actcatcgag
    14521 ccagaaacat ccagcagcag aatgcagggg caacgtggtt ccgggttact ggcaaaatcg
    14581 ctggtggcaa acgtgatttg ttcgctcata agaaatcctt tcttagagaa ttatcgaggt
    14641 ccggtatcat accgactgca ataatgaaat aagagcaaac gaagaaactt tcagaattag
    14701 gcccggcggc gcagaaaatt ttgctattcg cctcgcaaga acggagagcc gtcgattgac
    14761 actaagggcg gagtgacata atttcaggag tgagggttag ggagaggttt ccccctcccc
    14821 ctggtgttct tagtaagcct ggaagctaat cactaagagt atcaccagta tgatgacgtg
    14881 cttcatcata accctttcct tattaaaagc cctcttctcc gggaggggct ttcccgtttc
    14941 agcatcccgc tgaaatcgtc ggcttacctc ctttcgccct gaatacagtc tatcgctaac
    15001 gcgtgaacaa aatagtttcc tctgtgatta ttgcgtaacg actcgcaaag aatatttgct
    15061 ggcaggatcg cagactgcaa agcctgcgga ttgacaatct tatcgtgaag gcatactttc
    15121 aggagtgagg gtagagcggg gtttcccccg ccctggtagt cttagtaagc cgggaagctt
    15181 atgactaaga gcaccacgat gatgatccac ttcatcatga ccctttcctt atttatggcc
    15241 ccttcctcgg gaggggcttt cccgtttcag cgtcccgctg aaatcgtcgg cttacctcct
    15301 ttcgccatgc aagcagtcta tcgctaacgc gtagataaaa tagtttcatg tgttattact
    15361 ggatgcgtgc tcgcaaatgt gcccgtcatt cagacgattc cagacagtgt ttcataattc
    15421 ctccattttt ctcccttatt ggctggctac actagtatca ttccgcgaaa cgtttcagga
    15481 agagaaactc ttaacgatga aaggtagtta taaatcccgt tgggtaatcg taatcgtggt
    15541 ggttatcgcc gccatcgccg cattctggtt ctggcaaggc cgcaatgact cccggagtgc
    15601 agccccaggg gcgacgaaac aagcgcagca atcgccagcg ggtggtcgac gtggtatgcg
    15661 ttccggccca ttagccccgg ttcaggcggc gaccgccgta gaacaggcag ttccgcgtta
    15721 cctcaccggg cttggcacca ttaccgccgc taataccgtt acggtgcgca gccgcgtgga
    15781 cggccaactg atagcgttac atttccagga aggccagcag gtcaaagcag gcgatttact
    15841 ggcagaaatt gaccccagcc agttcaaagt tgcattagca caagcccagg gccaactggc
    15901 aaaagataaa gccacgcttg ccaacgcccg ccgtgacctg gcgcgttatc aacaactggc
    15961 aaaaaccaat ctcgtttccc gccaggagct ggatgcccaa caggcgctgg tcagtgaaac
    16021 cgaaggcacc attaaggctg atgaagcaag cgttgccagc gcgcagctgc aactcgactg
    16081 gagccggatt accgcaccag tcgatggtcg cgttggtctc aagcaggttg atgttggtaa
    16141 ccaaatctcc agtggtgata ccaccgggat cgtggtgatc acccagacgc atcctatcga
    16201 tttagtcttt accctgccgg aaagcgatat cgctaccgta gtgcaggcgc agaaagccgg
    16261 aaaaccgctg gtggtagaag cctgggatcg caccaactcg aagaaattaa gtgaaggcac
    16321 gctgttaagt ctagataacc aaatcgatgc cactaccggt acgattaaag tgaaagcacg
    16381 ctttaataat caggatgatg cgctgtttcc caatcagttt gttaacgcgc gcatgttagt
    16441 cgacaccgaa caaaacgccg tagtgatccc aacagccgcc ctgcaaatgg gcaatgaagg
    16501 ccattttgtc tgggtgctga atagcgaaaa caaggtcagc aaacatctgg tgacgccggg
    16561 cattcaggac agtcagaaag tggtgatccg tgcaggtatt tctgcgggcg atcgcgtggt
    16621 gacagacggc attgatcgcc tgaccgaagg ggcgaaagtg gaagtggtgg aagcccagag
    16681 cgccactact ccggaagaga aagccaccag ccgcgaatac gcgaaaaaag gagcacgctc
    16741 ctgatgcagg tgttaccccc gagcagcaca ggcggcccgt cgcgcctgtt tattatgcgt
    16801 cctgtggcca ccacgctgct gatggtggcg atcttactcg ccgggattat cggttatcgc
    16861 gccctgcccg tttcggcgct gccggaagtg gactatccga ccattcaggt ggtcacgctc
    16921 tacccaggtg ccagcccgga tgtcatgacc tctgccgtta ccgcgccgct agaacgccag
    16981 ttcgggcaga tgtctggcct gaaacagatg tcgtcgcaaa gttccggcgg tgcgtcagtt
    17041 atcactttgc agttccagct aacattaccg ctcgatgtcg ccgagcagga agtgcaggcc
    17101 gcgattaacg ctgcgaccaa cttgttgccg agcgatctgc ctaacccgcc ggtttacagc
    17161 aaagtgaacc cggcagatcc gccgatcatg acgctcgccg tcacctcaac cgccatgccg
    17221 atgacgcaag tggaagatat ggtggaaacc cgcgtcgcgc agaaaatctc gcagatttcc
    17281 ggcgtcggcc tggtgacgct ttccggcggt cagcgtccgg ctgttcgcgt caaacttaac
    17341 gctcaggcga ttgccgccct cggcctgacc agcgaaaccg tgcgcaccgc cattaccggc
    17401 gctaacgtta actcggcaaa aggtagcctc gacggccctt cccgtgcggt cacgctttcc
    17461 gcgaacgacc agatgcaatc cgccgaagag tatcgccagc taatcatcgc ctaccagaac
    17521 ggcgcgccaa ttcgtctggg cgatgtcgca actgtagagc aaggtgcaga aaacagctgg
    17581 ctcggcgcgt gggcgaacaa agaacaggcc attgtgatga atgttcagcg ccagcccggt
    17641 gctaacatta tctccaccgc cgacagcatt cggcagatgc tgccacagct cactgagagt
    17701 ctgccgaaat cggtgaaggt gacagtgctt tccgatcgca ccaccaatat ccgcgcatcc
    17761 gtcgatgata ctcagtttga attgatgatg gctatcgcgc tggtagtcat gattatctac
    17821 ctgtttttgc gtaatattcc ggcgaccatc attcccggtg ttgctgtacc gctgtcgtta
    17881 atcggcactt tcgcggttat ggtgtttctc gatttttcaa tcaataacct gacactgatg
    17941 gcgttaacta tcgccaccgg attcgtggtc gatgacgcca tcgtggtgat cgaaaacatt
    18001 tcccgctata tcgaaaaagg cgaaaaaccg ttggcggcgg cgctcaaggg cgcaggtgaa
    18061 atcggcttta ccattatctc gctgaccttc tcactgattg cggtgttgat cccactgctg
    18121 tttatgggcg atatcgtcgg gcgactgttc cgcgaatttg ctattaccct ggcggtagcg
    18181 attttgatct cagcggtggt gtcgctgacc ctgacaccga tgatgtgcgc gcggatgctc
    18241 agccaggagt cgttgcgtaa acagaaccgc ttctcccgtg cctcggaaaa aatgttcgac
    18301 aggataatcg ccgcctatgg tcgtggactg gcgaaagtgc tgaatcatcc gtggctgacc
    18361 ttaagcgtgg cactcagcac gctgctgctt agcgtgctgc tgtgggtgtt cattccgaaa
    18421 ggtttcttcc cggtacagga caatggcatt attcagggca ctttgcaggc accgcaatcc
    18481 agctcctttg ccaatatggc ccagcgacaa cgccaggtcg cggacgtgat tttgcaggat
    18541 ccggcagtgc aaagcctgac ctcatttgtt ggcgttgatg gcactaaccc gtcgctgaac
    18601 agtgcacgtt tacaaatcaa cctcaaaccg ttggatgaac gtgatgatcg ggtgcaaaaa
    18661 gtcatcgccc gtctgcaaac ggcggtagat aaagtgccgg gcgtcgatct cttcctgcaa
    18721 ccaacgcagg atctgactat tgatactcag gtcagccgca cccagtacca gtttaccttg
    18781 caggccacgt cactggatgc gctcagtacc tgggtgccac agttgatgga aaaactccag
    18841 caactgccac agctttctga tgtctccagc gactggcagg acaaagggct ggtggcgtat
    18901 gtcaatgttg atcgcgacag cgccagccgt ctggggatca gcatggcgga tgtcgataac
    18961 gccctgtaca acgcgtttgg tcagcggctg atttccacta tttatactca ggccaaccag
    19021 tatcgcgtgg tgctggagca caacaccgaa aatgccccag gcctcgcggc gctggatacc
    19081 attcgcctga ccagcagcga cggcggcgtg gtgccgctaa gctcaattgc caaaattgag
    19141 cagcgttttg cgccgctctc catcaaccat ctggatcagt tcccggtaac gaccatctcc
    19201 tttaacgtgc cggataacta ttcgctgggc gatgcggtgc aggcgattat ggacaccgaa
    19261 aagacgctga atctgccggt ggatatcacc acgcagttcc agggcagcac cctcgccttc
    19321 cagtcggcgc tgggcagcac tgtctggctg attgtcgcgg cggtggtggc gatgtatatc
    19381 gtgctcggca ttctgtacga gagctttatt cacccgatca ccattctctc gacgctaccc
    19441 accgcagggg ttggcgcact gctggcgttg ctgattgctg gtagcgaact ggatgtgatt
    19501 gcgattatcg gcattatttt gctgatcggt atcgtgaaga agaacgccat catgatgatc
    19561 gacttcgcgc tggctgccga gcgcgagcaa ggcatgtcgc cgcgcgaggc aatctaccag
    19621 gcttgtctgt tgcgttttcg tccgatcctg atgaccaccc tggcggctct gcttggcgcg
    19681 ctgccgctga tgttgagtac cggggtcggc gcggaactgc gtcgtccgtt aggtatcggc
    19741 atggtcggcg gcctgattgt cagccaggtg ctgacgctgt tcaccacgcc ggtgatttat
    19801 ctgctgttcg accgcctggc attgtggacc aaaagccgct ttgcccgtca tgaagaggag
    19861 gcgtaagtga agttttttgc cctcttcatt taccgtccgg tggcgacgat tttactgtcg
    19921 gttgccatta ccctgtgcgg catactgggc ttccgtatgc tgccggtcgc cccgctgccg
    19981 caggtcgatt ttccggtgat tatggtcagc gcctcgctgc ccggtgcgtc accagaaaca
    20041 atggcgtctt ccgttgccac gccgctggag cgctcacttg ggcgcattgc cggagtcagt
    20101 gaaatgacct ccagcagttc gctcggcagc acgcgtatta ttttgcagtt tgattttgac
    20161 cgggatatca acggcgcagc gcgtgatgtg caggcggcga tcaacgctgc acaaagtttg
    20221 ctgcccagtg ggatgcccag ccgcccgacc tatcgcaaag cgaacccgtc ggatgcgcca
    20281 attatgatcc tcacgctgac gtccgatact tattcgcagg gtgaactgta cgatttcgcc
    20341 tcgacgcagc tggctccgac gatttcgcaa atcgacggtg ttggtgatgt cgatgtcgga
    20401 ggcagctcac tgcccgccgt acgcgtcggg ctgaatccgc aggcgctgtt taatcagggc
    20461 gtgtcgctgg acgacgtacg caccgccgtc agcaatgcca acgtgcgtaa accgcagggc
    20521 gcgctggaag atggcactca ccgctggcag atccagacca atgatgagct aaaaaccgcc
    20581 gctgaatatc agccgttgat tattcactac aacaacggcg gcgcggttcg tctgggcgat
    20641 gtggcgacgg tgaccgactc agtgcaggat gtgcgcaacg ccgggatgac caacgccaaa
    20701 ccggctattt tactgatgat ccgcaaactg ccggaagcca atattatcca gacggttgac
    20761 agcatccggg caaaattacc ggagttgcag gaaaccattc cggcggcgat tgatctgcaa
    20821 attgcccagg atcgctcccc caccattcgc gcctcgctgg aagaagtcga gcaaacgctg
    20881 attatctcgg tggcgctggt gattctggtg gtgtttttat tcctgcgctc gggtcgcgcc
    20941 actattattc ccgccgtttc ggtgccggtt tcgctgattg gtacgtttgc ggcgatgtac
    21001 ctgtgcggat tcagtctcaa taacctttcg ttaatggcgc tcaccatcgc tactggtttc
    21061 gtggtggatg acgccatcgt ggtgctggaa aacattgcac gtcatctgga agcgggaatg
    21121 aaaccgttgc aagccgcact gcaaggtact cgcgaagtcg gttttacggt gctgtcgatg
    21181 agtctgtcac tggtggcggt gttcctgccg ctgctgttga tgggcggatt gccgggccga
    21241 ctgttacgcg aatttgccgt gacgctttct gtcgccattg gtatatcgtt gctggtttct
    21301 ctgacattaa cgccaatgat gtgtggctgg atgctgaaag ccagcaagcc gcgcgagcaa
    21361 aagcgactgc gtggttttgg tcgcatgttg gtagccctgc aacaaggcta cggcaagtca
    21421 ctgaaatggg tgctcaatca tacccgtctg gtgggcgtgg tgctgcttgg caccattgcg
    21481 ctgaatatct ggctgtatat ctcgatcccg aaaaccttct tcccggagca ggacactggc
    21541 gtgttgatgg gcgggattca ggcggatcag agtatttcgt ttcaggcgat gcgcggtaag
    21601 ttgcaggatt tcatgaaaat tatccgtgac gatccggcag tggataatgt caccggcttt
    21661 acaggcggtt cgcgagtgaa cagcgggatg atgtttatca ccctcaagcc acgcgacgaa
    21721 cgcagcgaaa cggcgcagca aattatcgac cgtctgcgcg taaaactggc gaaagaaccg
    21781 ggggcgaatc tgttcctgat ggcggtacag gatattcgcg ttggtgggcg tcagtcgaac
    21841 gccagctacc agtacacgtt gttatccgac gacctggcgg cactgcgaga atgggagccg
    21901 aaaatccgca aaaaactggc gacgttgccg gaactggcgg acgtgaactc cgatcagcag
    21961 gataacggcg cggagatgaa tctggtttac gaccgcgaca ccatggcacg gctgggaatc
    22021 gacgtacaag ccgccaacag tctgttaaat aacgccttcg gtcagcggca aatctcgacc
    22081 atttaccagc cgatgaacca gtataaagtg gtgatggaag tggatccgcg ctatacccag
    22141 gacatcagtg cgctggaaaa aatgttcgtt atcaataacg aaggcaaagc gatcccgctg
    22201 tcgtatttcg ctaaatggca accggcgaat gccccactat cggtgaatca tcagggatta
    22261 tcggcggcct cgaccatttc gtttaacctg ccgaccggaa aatcgctctc ggacgccagt
    22321 gcggcgatcg atcgcgcaat gacccagctt ggtgtgcctt cgacggtgcg cggcagtttt
    22381 gccggcacgg cgcaggtgtt ccaggagacg atgaactcgc aggtgatcct gattatcgcc
    22441 gccatcgcca cggtgtatat cgtgctgggt atcctttacg agagttacgt acatccgctg
    22501 acgattctct ccaccctgcc ctcggcgggc gttggagcgc tgttggcgct ggagctgttc
    22561 aatgccccgt tcagcctaat cgccctgata gggatcatgc tattaatcgg catcgtgaag
    22621 aaaaacgcca ttatgatggt cgattttgcg cttgaagccc aacggcacgg taacctgacg
    22681 ccgcaggaag ctattttcca ggcctgtctg ctgcgttttc gcccgattat gatgactacc
    22741 ctggcggcgc tgtttggtgc gctgccgctg gtattgtcgg gcggcgacgg ctcggagctg
    22801 cggcaacccc tggggatcac cattgtcggc ggactggtaa tgagccagct ccttacgctg
    22861 tataccacgc cggtggtgta tctctttttc gaccgtctgc ggctgcgttt ttcgcgtaaa
    22921 cctaaacaaa cggtaaccga gtaaatgaca gatcttcccg acagcacccg ttggcaattg
    22981 tggattgtgg ctttcggctt ctttatgcag tcgctggaca ccaccatcgt aaacaccgcc
    23041 cttccctcaa tggcgcaaag cctcggggaa agtccgttgc atatgcacat ggtcattgtc
    23101 tcttatgtgc tgaccgtggc ggtgatgctg cccgccagcg gctggctggc ggacaaagtc
    23161 ggcgtgcgca atattttctt taccgccatc gtgctgttta ctctcggttc actgttttgc
    23221 gcgctttccg gcacgctgaa cgaactgttg ctggcacgcg cgttacaggg cgttggcggc
    23281 gcgatgatgg tgccggtcgg cagattgacg gtgatgaaaa tcgtaccgcg cgagcaatat
    23341 atggcggcga tgacctttgt cacgttaccc ggtcaggtcg gtccgctgct cggtccggcg
    23401 ctcggcggtc tgctggtgga gtacgcatcg tggcactgga tctttttgat caacattccg
    23461 gtggggatta tcggtgcgat cgccacattg ctgttaatgc cgaactacac catgcagacg
    23521 cggcgctttg atctctccgg atttttattg ctggcggttg gcatggcggt attaaccctg
    23581 gcgctggacg gcagtaaagg tacaggttta tcgccgctga cgattgcagg cctggtcgca
    23641 gttggcgtgg tggcactggt gctttatctg ctgcacgcca gaaataacaa ccgtgccctg
    23701 ttcagtctga aactgttccg tactcgtacc ttttcgctgg gcctggcggg gagctttgcc
    23761 ggacgtattg gcagtggcat gttgcccttt atgacaccgg ttttcctgca aattggcctc
    23821 ggtttctcgc cgtttcatgc cggactgatg atgatcccga tggtgcttgg cagcatggga
    23881 atgaagcgaa ttgtggtaca ggtggtgaat cgctttggtt atcgtcgggt actggtagcg
    23941 accacgctgg gtctgtcgct ggtcaccctg ttgtttatga ctaccgccct gctgggctgg
    24001 tactacgttt tgccgttcgt cctgttttta caagggatgg tcaactcgac gcgtttctcc
    24061 tccatgaaca ccctgacgct gaaagatctc ccggacaatc tggcgagcag cggcaacagc
    24121 ctgctgtcga tgattatgca attgtcgatg agtatcggcg tcactatcgc cgggctgttg
    24181 ctgggacttt ttggttcaca gcatgtcagc gtcgacagcg gcaccacaca aaccgtcttt
    24241 atgtacacct ggcttagcat ggcgttgatc atcgcccttc cggcgttcat ctttgccaga
    24301 gtgccgaacg atacgcatca aaatgtagct atttcgcggc gaaaaaggag cgcgcaatga
    24361 agttctggcg acccggtatt accggcaaac tgtttctggc gattttcgcc acctgcattg
    24421 tcttgctgat cagtatgcac tgggcggtgc gtatcagttt tgagcgtggc tttattgatt
    24481 acatcaagca tggtaatgaa cagcgattac aactgttaag tgatgcgctt ggcgagcagt
    24541 atgcgcagca tggcaactgg cgcttcctgc gcaacaatga tcgctttgtc tttcagatcc
    24601 tgcgttcatt tgaacacgat aattcggaag ataaacccgg cccgggtatg ccaccgcacg
    24661 gctggcgtac ccagttctgg gtggttgatc aaaacaacaa agtgctggtt ggtccgcgag
    24721 cgccgattcc acctgacggt acacggcgac ccattctggt caacggtgcg gaagttggcg
    24781 cggtgatcgc ctcccccgtt gagcggttaa cgcgcaatac tgatatcaat ttcgataaac
    24841 aacagcggca aaccagctgg ttgattgtcg ccctggcaac gttactcgcg gcacttgcca
    24901 cttttctgct ggcgcgcggt ttactggcac cggtaaaacg acttgtcgat ggcacgcaca
    24961 aactggcggc gggcgatttc actacccgcg taacgcccac cagtgaagat gaactgggca
    25021 aactggcgca agacttcaac cagcttgcca gcacactgga gaaaaaccag caaatgcggc
    25081 gcgattttat ggccgatatt tctcacgaac tgcgtacgcc attagcggtg ctgcgcggtg
    25141 aactggaagc cattcaggat ggcgtgcgta aattcacgcc ggagacggtg gcgtctttac
    25201 aggcggaggt cggtacactg accaaactgg ttgacgatct ccatcagttg tcgatgtctg
    25261 atgaaggcgc tctcgcctat caaaaagcac cggtagattt gatcccactg ctggaagtgg
    25321 cgggcggcgc atttcgcgaa cgattcgcca gtcgtggcct gaaactgcaa ttttccctgc
    25381 cagacagtat taccgtattt ggcgatcgcg accgtttaat gcagttattc aataacttac
    25441 tggaaaacag cctgcgctac actgacagcg gcggcagcct gcaaatctct gccgggcagc
    25501 gcgacaaaac ggtgcgcctg acctttgccg acagtgcgcc aggtgtcagt gacgatcagc
    25561 tacaaaaatt gtttgaacgt ttttatcgca ccgaaggttc ccgcaaccgt gccagcggcg
    25621 gttccgggct ggggctggcg atttgcctga acattgttga agcacataat ggtcgcatta
    25681 ttgctgccca ttcgcctttt ggcggggtaa gcattacagt agagttaccg ctggaacggg
    25741 atttacagag agaagtatga ccgagttacc aatcgacgaa aacacaccgc gtattttgat
    25801 cgtggaagat gaaccgaagc tggggcagtt gctcattgat tatctgcgtg ctgcgagcta
    25861 tgcgccgacg cttatcagcc acggcgatca ggtactgccg tatgtgcgcc agacaccacc
    25921 ggatctgatc ctgttagatc tgatgctccc tggcaccgat ggcctgacgc tgtgccggga
    25981 aattcgtcgt ttttctgaca ttccgatcgt gatggtgacg gcaaaaatcg aagagatcga
    26041 tcgcctgctg gggctggaga ttggcgcaga tgattatatc tgtaagccgt acagcccacg
    26101 ggaagtggta gcgcgcgtca aaaccatttt gcgccgttgc aaaccgcagc gcgagttgca
    26161 gcaacaggat gctgaaagcc cgttgattat cgacgaaggt cgttttcagg cttcatggcg
    26221 cggtaaaatg cttgacctga cgcctgcgga atttcgtctg ctgaaaacgc tctctcacga
    26281 accaggaaaa gtgttctccc gcgagcaatt gctcaatcat ctttatgacg actaccgcgt
    26341 agtaaccgac cgcaccatcg acagccacat taaaaacctg cgccgcaagc tggaatctct
    26401 cgacgccgaa cagtcattta tccgcgccgt ttatggcgtc ggttaccgct gggaagccga
    26461 cgcctgccgc atcgtttagt tttagcgaca ttattttgtt agccggagat ggctttcaga
    26521 ccagatccgg caacattatc ccacgcatgg tcagcaaact gacattatct ctccgccctg
    26581 gcaggcatca gaaaatcgct catactttaa tcggtaaaca gcacctttag atgctgtttt
    26641 cgatacacaa tttcaatcaa ggagtcatta tggctggttg gtttgaactc agtaagagca
    26701 gtgataatca gttccggttt gtgctaaaag cgggcaatgg tgagactatc ctcaccagcg
    26761 agctttatac ctcaaaagcc tctgcggaaa agggcatcgc gtcggtgcgt agcaacagcc
    26821 cgcaagaaga acgctatgag aaaaaaacgg caagtaacgg caaattctat ttcaatctga
    26881 aagccgctaa tcatcaaatt atcggctcca gccagatgta cgccaccgcg caatctcgtg
    26941 aaaccggaat tgcctccgtt aaagccaatg gcacaagcca gacggtgaaa gacaatacgt
    27001 aattacgatg ccgggcgtgg gttgttggcg tccggcgctt taccagatcc ccccacagcg
    27061 ctacaatgcc cgcccttaaa gtgggggcac tcccctaacc gcttcatcag gtgaagcgga
    27121 tctgacctgt catcagaacg agagaattat gtttaaaccg gaactccttt ccccggcggg
    27181 aacgctgaaa aatatgcgtt acgctttcgc ttatggcgca gatgctgttt atgcgggcca
    27241 gccacgttac tccctgcgtg tgcgcaacaa cgaattcaac cacgaaaatc ttcagctcgg
    27301 catcaatgaa gcccacgcgc tggggaaaaa gttttatgtc gtggtcaaca ttgcaccgca
    27361 caacgccaag ctgaaaacct ttattcgtga cctgaaaccg gtggtggaaa tggggccgga
    27421 tgcgctgatt atgtccgatc cagggctgat tatgctggtg cgtgagcact tccctgaaat
    27481 gccgatccac ctctcggtgc aggctaacgc cgtaaactgg gcgacggtga aattctggca
    27541 gcaaatgggc ctgacccgcg tgatcctctc tcgcgagctg tcgctggaag agattgaaga
    27601 gatccgcaat caggtgccgg atatggagat cgagatcttc gttcacggcg cactgtgcat
    27661 ggcctactcc ggtcgctgcc tgctctctgg ctatatcaac aagcgcgatc cgaaccaggg
    27721 tacctgcacc aacgcctgcc gctgggagta caacgtccag gaagggaaag aagatgacgt
    27781 tggcaacatc gtacacaagt acgagccgat tccggtgcaa aatgttgagc cgacgctggg
    27841 tatcggcgca ccaaccgaca aagtgtttat gatcgaagaa gcccagcgtc cgggcgagta
    27901 tatgaccgcg tttgaagatg agcacggcac ttacatcatg aactcgaaag atctgcgcgc
    27961 catcgcccat gtagaacgcc tgaccaaaat gggcgtgcat tcgctgaaaa tcgaaggccg
    28021 taccaaatct ttctactatt gcgcacgcac cgcacaggtt taccgcaaag ctatcgatga
    28081 tgccgctgcg ggcaaaccgt tcgataccag cctgctggaa actctggaag gtctggcgca
    28141 tcgtggctat accgaaggtt tcctgcgtcg tcatactcac gacgattatc agaactacga
    28201 atacggttat tcagtttctg accgccagca gtttgttggt gagtttaccg gtgagcgcaa
    28261 gggggacctc gcagcggtag cggtgaaaaa taaattctcc gttggcgaca gccttgagct
    28321 gatgacgccg caaggcaaca tttactttac ccttgagcac atggaaaacg ccaaaggtga
    28381 agcgatgccg gtcgcaccag gcgatggtta tactgtgtgg ctcccggtcc cgcaggatct
    28441 tgagctaaat tacgctctcc tgatgcgtaa tttctctggg gaaaccacgc gtaaccccca
    28501 cggtaagtga ttaatttcga ttatttttcc cggatggaaa attcttagaa accgatcaca
    28561 tacagctgca tttattaagg ttatcatccg tttcgctgaa aaacataacc cataaaatgc
    28621 tagctgtacc aggaaccacc tccttagcct gtgtaatctc ccttacacgg gcttattttt
    28681 tatgcataag ccctatctct ggtcaccgtc ttccattgac cacatcgata gaatcctcct
    28741 tcatagcacg atgcctttca cttatcggca tcgtgctccc acaggttccg gctacgcaca
    28801 gccagaacgc gcatatttga cgcttaccaa aaaatattct cactctccac atttgaatgt
    28861 cagacgagcg acgccatgta atcctgcacc ttctgtcttc aggtcaacta tctgcatttt
    28921 tttgccctga gtaacacaga aatgggctgc atcatttttt actatatttt ctgcaccaga
    28981 tattctgccc ctggctaaag aagcttcggc ttcggtgtag tattggttat cgagtttacg
    29041 ctgaatatta cttttatatg caagaccaaa tttaccgata cttgtctcat
//
LOCUS       DAPHBJ010000037        29050 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.037, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000037 DAPHBJ010000000
VERSION     DAPHBJ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.037"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(42..1517)
                     /gene="ampG"
                     /locus_tag="RHZ76_001494"
     CDS             complement(42..1517)
                     /gene="ampG"
                     /locus_tag="RHZ76_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HDV2812154.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(1561..2139)
                     /locus_tag="RHZ76_001495"
     CDS             complement(1561..2139)
                     /locus_tag="RHZ76_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV2812155.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            2444..2761
                     /gene="bolA"
                     /locus_tag="RHZ76_001496"
     CDS             2444..2761
                     /gene="bolA"
                     /locus_tag="RHZ76_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HDV2812156.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            3105..4403
                     /gene="tig"
                     /locus_tag="RHZ76_001497"
     CDS             3105..4403
                     /gene="tig"
                     /locus_tag="RHZ76_001497"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDV2812157.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            4649..5272
                     /gene="clpP"
                     /locus_tag="RHZ76_001498"
     CDS             4649..5272
                     /gene="clpP"
                     /locus_tag="RHZ76_001498"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDV2812158.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            5398..6672
                     /gene="clpX"
                     /locus_tag="RHZ76_001499"
     CDS             5398..6672
                     /gene="clpX"
                     /locus_tag="RHZ76_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HDV2812159.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            6860..9214
                     /gene="lon"
                     /locus_tag="RHZ76_001500"
     CDS             6860..9214
                     /gene="lon"
                     /locus_tag="RHZ76_001500"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDV2812160.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            9423..9695
                     /gene="hupB"
                     /locus_tag="RHZ76_001501"
     CDS             9423..9695
                     /gene="hupB"
                     /locus_tag="RHZ76_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HDV2812161.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            9887..11758
                     /gene="ppiD"
                     /locus_tag="RHZ76_001502"
     CDS             9887..11758
                     /gene="ppiD"
                     /locus_tag="RHZ76_001502"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDV2812162.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQKISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            11909..12280
                     /locus_tag="RHZ76_001503"
     CDS             11909..12280
                     /locus_tag="RHZ76_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDV2812163.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            12374..12772
                     /gene="fadM"
                     /locus_tag="RHZ76_001504"
     CDS             12374..12772
                     /gene="fadM"
                     /locus_tag="RHZ76_001504"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HDV2812164.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(12824..13519)
                     /gene="queC"
                     /locus_tag="RHZ76_001505"
     CDS             complement(12824..13519)
                     /gene="queC"
                     /locus_tag="RHZ76_001505"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDV2812165.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(13584..15284)
                     /locus_tag="RHZ76_001506"
     CDS             complement(13584..15284)
                     /locus_tag="RHZ76_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HDV2812166.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLVHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLSAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15384..16202
                     /gene="cof"
                     /locus_tag="RHZ76_001507"
     CDS             15384..16202
                     /gene="cof"
                     /locus_tag="RHZ76_001507"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HDV2812167.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            16355..16813
                     /gene="decR"
                     /locus_tag="RHZ76_001508"
     CDS             16355..16813
                     /gene="decR"
                     /locus_tag="RHZ76_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HDV2812168.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            16843..18615
                     /locus_tag="RHZ76_001509"
     CDS             16843..18615
                     /locus_tag="RHZ76_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDV2812169.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENREEAVDA"
     gene            18608..20389
                     /locus_tag="RHZ76_001510"
     CDS             18608..20389
                     /locus_tag="RHZ76_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDV2812170.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            20570..20908
                     /gene="glnK"
                     /locus_tag="RHZ76_001511"
     CDS             20570..20908
                     /gene="glnK"
                     /locus_tag="RHZ76_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDV2812171.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            20938..22224
                     /gene="amtB"
                     /locus_tag="RHZ76_001512"
     CDS             20938..22224
                     /gene="amtB"
                     /locus_tag="RHZ76_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HDV2812172.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEDVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(22273..23133)
                     /gene="tesB"
                     /locus_tag="RHZ76_001513"
     CDS             complement(22273..23133)
                     /gene="tesB"
                     /locus_tag="RHZ76_001513"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HDV2812173.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            23351..23923
                     /locus_tag="RHZ76_001514"
     CDS             23351..23923
                     /locus_tag="RHZ76_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HDV2812174.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(23954..24265)
                     /locus_tag="RHZ76_001515"
     CDS             complement(23954..24265)
                     /locus_tag="RHZ76_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HDV2812175.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            24428..24524
                     /gene="ffs"
                     /locus_tag="RHZ76_001516"
     ncRNA           24428..24524
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="RHZ76_001516"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            24644..24997
                     /locus_tag="RHZ76_001517"
     CDS             24644..24997
                     /locus_tag="RHZ76_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HDV2812176.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(25039..26589)
                     /gene="pdeB"
                     /locus_tag="RHZ76_001518"
     CDS             complement(25039..26589)
                     /gene="pdeB"
                     /locus_tag="RHZ76_001518"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HDV2812177.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVAYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     LPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESPVLTSEKIPQLLRDMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(26753..27223)
                     /locus_tag="RHZ76_001519"
     CDS             complement(26753..27223)
                     /locus_tag="RHZ76_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HDV2812178.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(27339..27890)
                     /gene="maa"
                     /locus_tag="RHZ76_001520"
     CDS             complement(27339..27890)
                     /gene="maa"
                     /locus_tag="RHZ76_001520"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HDV2812179.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(28062..28280)
                     /gene="hha"
                     /locus_tag="RHZ76_001521"
     CDS             complement(28062..28280)
                     /gene="hha"
                     /locus_tag="RHZ76_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HDV2812180.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(28306..28680)
                     /gene="tomB"
                     /locus_tag="RHZ76_001522"
     CDS             complement(28306..28680)
                     /gene="tomB"
                     /locus_tag="RHZ76_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HDV2812181.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         7295 a         6994 c         7836 g         6925 t
ORIGIN      
        1 attacggcga gtgattacta cagctaaata atatttacag attacgtcag atgcgttttt
       61 cgtagcgcca gataatccag caaaccacca agcacgacgc cagaaagggc gactaaaacc
      121 ccgacttcca gcagagcagg caggaaagag aagtgcgtca aatccagcgc gtccatcgtc
      181 aacagcagta accacacggc caacaggctg acgcccgccg ccagtgtcca catggcaaag
      241 gcataacctg ccggatattc ggtacgggag ataaagttgt catttactcg tgtatattca
      301 agcgtctggc ggcaaaccag cagcaaaata agccccggta cggcagcggc gacggagaat
      361 agatagaatg tcgaccagcc gtgtgcttca acaaaccaac ccgccacggg gccgacataa
      421 actcgtccta cagcggaaag cgctgagagc agggcaaatt gagttgcgga aaatgactta
      481 ttacacagcg tcattaacag cgcgacaaag gctgatgtgc ccatcccgcc acagaggttt
      541 tcgaaaaaga cggctgcgcc catgctgtag agatgcttat cagtaatcga cagcagccag
      601 taaccagcgt tagacgcacc ttgtaaaatg ccgaaaatca gcagcgcccg gaacagcgac
      661 aggcgctgca tcaaaatccc accgtacaat gcgcccacaa tggtcgccag taagccaagc
      721 gttttgttaa ccacgcctac ttcacccgca tcaaacccga cgccgcgaat caaaaacgtg
      781 gttgtcaggc tcatggcgaa tgcgtcgccc agcttataca gcacgataag aagcaaaata
      841 agccaggcat tattgcgacc aaagaaatct cgcagaggtg caacaaccgc ttgttccagc
      901 gtttttggca caggaatggt gtcggtcggt tctggtgcaa gcaacgtcgc gataatacaa
      961 gggatcaaca gcgccgccat caaccagtac atgccctgcc agcccagcca tttatctgcc
     1021 agccacaggg ctaagccgcc ggaaaccagc atccctaaac ggtaacccag cacgctgatt
     1081 gccgcgcccg caccacgttc ttctgccggg agcacatcgg ttttccacgc atcgaagaca
     1141 atatcctgag aggccgagca aaaagcgatc accaccgcca gcgccgccat ccagcggagt
     1201 tgggtgcctg gttcgagaaa acccatcgcc gcaatggcga ctaataacag gatttgcgtg
     1261 gcgagcagcc aaccgcgccg ccgcccaaaa aatgggggcg tgtagcggtc catcagcggt
     1321 gaccagagga atttaaaaac gtaagcctgg cctaccagag agaagaaacc aatggttttg
     1381 agatcaatat tctcgaccgt catccaggcc tgtaaggtac cggatgtcag ggcgagcggt
     1441 agcccggaag caaaacccag gatcagcaat atggctgaac gcggctgttg aaaaatacgt
     1501 aaatattgac tggacatggg cgtctacagg cccggcttgc accgggccag agggcagaaa
     1561 ttaacgcgcg ttctgcttga tgaattcgtg gatgctggtg tcctgagaca tatcagcgat
     1621 ggtatcggtc agcacactgt taaccgcatc ggcgatattt ttgttggagg cctggaacgc
     1681 accttcaacg ttgtagctgg cacgatagtt tttggtcatt ttgttgccat tctgcgcggt
     1741 agcgatgatg gcgatatccg ctttggtcgc gatgttgtag cgcacgttgc cctgggacac
     1801 gtcagcatac agttggctaa cgatgatttg cagattaacc gggccattcg gaccaaccat
     1861 gtaaccacgc gcggtcatct gtttttccag cacttcttgc agcaggaaac gcagatcgcg
     1921 ggaggcggtc agggtaacga tttgattatc gcgggtgact tttgccagcg cctgatcggt
     1981 acgctgatcg gcaccattaa tgcttacggt gacgcccatc aggcttggat cctgctgtgg
     2041 cagtgtaatc gtcggggaaa cttcaatagt tgttggcggt tttgcgcatc ctgcaagcat
     2101 aaacagagca actaacggga agaggatttt tttgaacatg ttcgggctct cagagactct
     2161 taagcgtgtt tggtaaaaat tcccgccatc ataacattgc caacggcgag gggaagtggg
     2221 taaggcatgt aaattcatca tgttgacgaa ataatcgccc ctggtaaaag aaacactgat
     2281 gcgaggcctg tgtttcaatc tttaaatcag taaacttcat acgcttgacg gaaaaaccag
     2341 gacgaaacct aaatatttgt tgttaagctg caatggaaac ggtaaaagcg gctagtattt
     2401 aaagggatgg atgacatctc agcgttgtcg gaggagatat ttcatgatga tacgtgagcg
     2461 gatagaagaa aaattaaggg cggcgttcca acccgtattc ctcgaagtag tggatgaaag
     2521 ctatcgtcac aatgtcccag ccggctctga aagccatttt aaagttgtgc tggtcagcga
     2581 tcgttttacg ggtgaacgtt ttctgaatcg tcatcgaatg atttacagta ctttagcgga
     2641 ggaactctct actaccgttc atgcgctggc tctgcatact tacactatta aggagtggga
     2701 agggttgcag gacaccgtct ttgcctctcc tccctgtcgt ggagcaggaa gcatcgcgta
     2761 aaaacgcatt tgcaactgtc ggcgcttttc cagtatgttg ctaaagattt tatgaaaaac
     2821 ggcctgcggg ccgttttgtt ttgtctggat tttgcgcttt ttgcccagca ttcagacgaa
     2881 aattgcccgg gaattgtgaa aaaatacgcg acagcgcgca ataaccgttc tcgactcata
     2941 aaagtgatgc cgctataatg ccgcgtccta tttgaatgct ttcgggatga ttctggtaac
     3001 agggaatgtg attgattata agaacatccc ggttccgcga agccaacaac ctgtgcttgc
     3061 ggggtaagag ttgaccgagc actgtgattt tttgaggtaa caagatgcaa gtttcagttg
     3121 aaaccactca aggccttggc cgccgtgtaa cgattactat cgctgctgac agcatcgaga
     3181 ccgctgttaa aagcgagctg gtcaacgttg cgaaaaaagt acgtattgac ggcttccgca
     3241 aaggcaaagt gccaatgaat atcgttgctc agcgttatgg cgcgtctgta cgccaggacg
     3301 ttctgggtga cctgatgagc cgtaacttca ttgacgccat cattaaagaa aaaatcaatc
     3361 cggctggcgc accgacttat gttccgggcg aatacaagct gggtgaagac ttcacttact
     3421 ctgtagagtt tgaagtttat ccggaagttg aactgcaggg tctggaagcg atcgaagttg
     3481 aaaaaccgat cgttgaagtg accgacgctg acgttgacgg catgctggat actctgcgta
     3541 aacagcaggc gacctggaaa gaaaaagacg gcgctgttga agcagaagac cgcgtaacca
     3601 tcgacttcac cggttctgta gacggcgaag agttcgaagg cggtaaagcg tctgatttcg
     3661 tactggcgat gggccagggt cgtatgatcc cgggctttga agacggtatc aaaggccaca
     3721 aagctggcga agagttcacc atcgacgtga ccttcccgga agaataccac gcagaaaacc
     3781 tgaaaggtaa agcagcgaaa ttcgctatca acctgaagaa agttgaagag cgtgaactgc
     3841 cggaactgac tgcagaattc atcaaacgtt tcggcgttga agatggttcc gtagaaggtc
     3901 tgcgcgctga agtgcgtaaa aacatggagc gcgagctgaa gagcgccatc cgtaaccgcg
     3961 ttaagtctca ggcgatcgaa ggtctggtaa aagctaacga catcgacgta ccggctgcgc
     4021 tgatcgacag cgaaatcgac gttctgcgtc gccaggctgc acagcgtttc ggtggcaacg
     4081 aaaaacaagc tctggaactg ccgcgcgaac tgttcgaaga acaggctaaa cgccgcgtag
     4141 ttgttggcct gctgctgggc gaagttatcc gcaccaacga gctgaaagct gacgaagagc
     4201 gcgtgaaagg cctgatcgaa gagatggctt ctgcgtacga agatccgaaa gaagttatcg
     4261 agttctacag caaaaacaaa gaactgatgg acaacatgcg caatgttgct ctggaagaac
     4321 aggctgttga agctgtactg gcgaaagcga aagtgactga aaaagaaacc actttcaacg
     4381 aactgatgaa ccagcaggcg taatttacgc agcataacgc gctaaattcg cacaaaggcc
     4441 cgtcaccgcc aggtggtggg cttttttttg tcatgaattt tgcatggaac cgtgcgaaaa
     4501 gcctctttcg gtgttagcgt aacaacaaaa gattgttatg cttgaaatat ggtgatgccg
     4561 tacccataac acagggacta gctgataatc cgtccataag gttacaatcg gtacagcagg
     4621 ttttttcaat tttatccagg agacggaaat gtcatacagc ggcgaacgag ataactttgc
     4681 accccatatg gcgctggtgc cgatggtcat tgaacagacc tcacgcggtg agcgctcttt
     4741 tgatatctat tctcgtctac ttaaggaacg cgtcattttt ctgactggcc aggttgaaga
     4801 ccacatggct aacctgattg tggcgcagat gctgttcctg gaagcggaaa acccagaaaa
     4861 agatatctat ctgtacatta actccccagg cggggtgatc actgccggga tgtctatcta
     4921 tgacaccatg cagtttatca agcctgatgt cagcaccatc tgtatgggcc aggcggcctc
     4981 gatgggcgct ttcttgctga ccgcaggggc aaaaggtaaa cgtttttgcc tgccgaattc
     5041 gcgcgtgatg attcaccaac cgttgggcgg ctaccagggc caggcgaccg atatcgaaat
     5101 tcatgcccgt gaaattctga aagttaaagg gcgcatgaat gaacttatgg cgcttcatac
     5161 gggtcaatca ttagaacaga ttgaacgtga taccgagcgc gatcgcttcc tttccgcccc
     5221 tgaagcggtg gaatacggtc tggtcgattc gattctgacc catcgtaatt gatgccagag
     5281 gcgcaactgt gccgctatac ttatccaggg cggcacaacg ctgtaagcgg cttgcgcctg
     5341 agaatggcat ttgcgtcgtc gtgtgcggca caaagaacaa agaagaggtt ttgacccatg
     5401 acagataaac gcaaagatgg ctcaggcaaa ttgctgtatt gctctttttg cggcaaaagc
     5461 cagcatgaag tgcgcaagct gattgccggt ccatccgtgt atatctgcga cgaatgtgtt
     5521 gatttatgta acgacatcat tcgcgaagag attaaagaag ttgcaccgca tcgtgaacgc
     5581 agtgcgctac cgacgccgca tgaaattcgc aaccacctgg acgattacgt tatcggccag
     5641 gaacaggcga aaaaagtgct ggcggtcgcg gtatacaacc attacaaacg tctgcgcaac
     5701 ggcgatacca gcaatggcgt cgagttgggc aaaagtaaca ttctgctgat cggtccgacc
     5761 ggttccggta aaacgctgct ggctgaaacg ctggcgcgcc tgctggatgt tccgttcacc
     5821 atggccgacg cgactacact gaccgaagcc ggttatgtgg gtgaagacgt tgaaaacatc
     5881 attcagaagc tgttgcagaa atgcgactac gatgtccaga aagcacagcg tggtattgtc
     5941 tacatcgatg aaatcgacaa gatttctcgt aagtcagaca acccgtccat tacccgagac
     6001 gtttccggtg aaggcgtaca gcaggcactg ttgaaactga tcgaaggtac ggtagctgct
     6061 gttccaccgc aaggtgggcg taaacatccg cagcaggaat tcttgcaggt tgatacctct
     6121 aagatcctgt ttatttgtgg cggtgcgttt gccggtctgg ataaagtgat ttcccatcgt
     6181 gtagaaaccg gctccggcat tggttttggc gcgacggtaa aagcgaagtc cgacaaagca
     6241 agcgaaggcg agctgctggc gcaggttgaa ccggaagatc tgatcaagtt tggtctgatc
     6301 cctgagttca ttggtcgtct gccggttgtc gcaacgttga atgaactgag cgaagaagct
     6361 ctgattcaga tcctcaaaga gccgaaaaac gccctgacca agcagtatca ggcgctgttt
     6421 aatctggaag gcgtggatct ggaattccgt gacgaggcgc tggatgctat cgctaagaaa
     6481 gcgatggcgc gtaaaaccgg tgcccgtggc ctgcgttcca tcgtagaagc cgcactgctc
     6541 gataccatgt acgatctgcc gtccatggaa gacgtcgaaa aagtggttat cgacgagtcg
     6601 gtaattgatg gtcaaagcaa accgttgctg atttatggca agccggaagc gcaacaggca
     6661 tctggtgaat aattaaccat tcccatacaa ttagttaacc aaaaaggggg gattttatct
     6721 cccctttaat ttttcctcta ttctcggcgt tgaatgtggg ggaaacatcc ccatatactg
     6781 acgtacatgt taatagatgg cgtgaagcac agtcgtgtca tctgattacc tggcggaaat
     6841 taaactaaga gagagctcta tgaatcctga gcgttctgaa cgcattgaaa tccccgtatt
     6901 gccgctgcgc gatgtggtgg tttatccgca catggtcatc cccttatttg tcgggcggga
     6961 aaaatctatc cgttgtctgg aagcggcgat ggaccatgat aaaaaaatta tgctggtcgc
     7021 gcagaaagaa gcttcaacgg atgagccggg tgtaaacgat cttttcaccg tcgggaccgt
     7081 ggcctctata ttgcagatgc tgaagctgcc tgacggcacc gtcaaagtgc tggtcgaggg
     7141 gttacagcgc gcgcgtattt ctgcgctctc tgacaatggc gaacactttt ctgcgaaggc
     7201 ggagtatctg gagtcgccga ccattgatga gcgagaacag gaagtgctgg tgcgtactgc
     7261 aatcagccag ttcgaaggct acatcaagct gaacaaaaaa atcccaccag aagtgctgac
     7321 gtcgttgaat agcatcgacg atccggcgcg tctggcggat accattgctg cacatatgcc
     7381 gctgaaactg gctgacaaac agtccgttct ggagatgtcc gacgttaacg aacgtctgga
     7441 atatctgatg gcaatgatgg aatcggaaat cgatctgctg caggttgaga aacgcattcg
     7501 caaccgcgtt aaaaagcaga tggagaaatc ccagcgtgag tactatctga acgagcaaat
     7561 gaaagctatt cagaaagaac tcggtgaaat ggacgacgcg ccggacgaaa acgaagccct
     7621 gaagcgcaaa atcgacgcgg cgaagatgcc gaaagaagca aaagagaaag cggaagcaga
     7681 gttgcagaag ctgaaaatga tgtctccgat gtcggcagaa gcgaccgtag tgcgtggtta
     7741 tatcgactgg atggtacagg tgccgtggaa tgcgcgtagc aaggtcaaaa aagacctgcg
     7801 tcaggcgcag gaaatccttg ataccgacca ttatggtctg gagcgcgtga aagatcgaat
     7861 ccttgagtat cttgcggttc aaagccgtgt caacaaaatc aagggaccga tcctctgcct
     7921 ggtagggccg ccgggggtag gtaaaacctc tcttggtcag tccattgcca aagccaccgg
     7981 gcgtaaatat gtccgtatgg cgctgggcgg cgtgcgtgat gaagcggaaa tccgtggtca
     8041 ccgccgtact tacatcggtt ctatgccggg taaactgatc cagaaaatgg cgaaagtggg
     8101 cgtgaaaaac ccgctgttcc tgctcgatga gatcgacaaa atgtcttctg acatgcgtgg
     8161 cgatccggcc tctgcactgc ttgaagtgct ggatccagag cagaacgtag cgttcagcga
     8221 ccactacctg gaagtggatt acgatctcag cgacgtgatg tttgtcgcga cgtcgaactc
     8281 catgaacatt ccggcaccgc tgctggatcg tatggaagtg attcgcctct ccggttatac
     8341 cgaagatgaa aaactgaaca tcgccaaacg tcacctgctg ccgaagcaga ttgaacgtaa
     8401 tgcactgaaa aaaggtgagc tgaccgtcga cgatagcgcc attatcggca ttattcgtta
     8461 ctacacccgt gaggcgggcg tgcgtggtct ggagcgtgaa atctccaaac tgtgtcgcaa
     8521 agcggttaag cagttactgc tcgataagtc attaaaacat atcgaaatta acggcgataa
     8581 cctgcatgac tatctcggtg ttcagcgttt cgactatggt cgcgcggata acgaaaaccg
     8641 tgtcggtcag gtaaccggtc tggcgtggac ggaagtgggc ggtgacttgc tgaccattga
     8701 aaccgcatgt gttccgggta aaggcaaact gacctatacc ggttcgctcg gcgaagtgat
     8761 gcaggagtcc attcaggcgg cgttaacggt ggttcgtgcg cgtgcggaaa aactggggat
     8821 caaccctgat ttttacgaaa aacgcgacat ccacgtccac gtaccggaag gtgcgacgcc
     8881 gaaagatggt ccgagtgccg gtattgctat gtgcaccgcg ctggtttctt gcctgaccgg
     8941 taacccggtt cgtgccgatg tggcaatgac cggtgagatc actctgcgtg gtcaggtact
     9001 gccgatcggt ggtttgaaag aaaaactcct ggcagcgcat cgcggcggga ttaaaacagt
     9061 gttaattccg ttcgaaaata aacgcgatct ggaagagatt cctgacaacg taattgccga
     9121 tctggacatt catcctgtga agcgcattga ggaagttctg actctggcgc tgcaaaatga
     9181 accgtctggc atgcaggttg tgactgcaaa atagtgacct cgcgcaaaat gcactaataa
     9241 aaacagggct ggcaggctaa ttcgggcttg ccagcctttt tttgtctcgc taagttagat
     9301 ggcggatcgg gcttgccctt attaaggggt gttgtaaggg gatggctggc ctgatataac
     9361 tgctgcgcgt tcgtaccttg aaggattcaa gtgcgatata aattataaag aggaagagaa
     9421 gagtgaataa atctcaattg atcgacaaga ttgctgcagg ggctgatatc tctaaagctg
     9481 cggctggccg tgcgttagat gctattattg cttccgtaac tgaatctctg aaagaagggg
     9541 atgatgtagc actggtaggt tttggtactt ttgccgttaa agagcgtgct gcccgtactg
     9601 gccgcaaccc gcagaccggt aaagagatca ccatcgctgc tgctaaagta ccgagcttcc
     9661 gtgcaggtaa agcactgaaa gacgcggtaa actaagcgtt gtccccagtg gggatgtgac
     9721 gaagttcaag ggcgcatcta ctgatgtgcc ttttttattt gtattcggtg actttctgcg
     9781 tcttgtgggc tgacaattgc ccccgtttct tgtcacaata ggcctttgcg cgcatcgata
     9841 cgttgcgtga ggtacacagt catctacagc ggagtgttgt tacaccatga tggacagctt
     9901 acgcacggct gcaaacagtc tcgtgctcaa gattattttc ggtatcatta tcgtgtcgtt
     9961 catattgacc ggcgtgagtg gttacctgat tggcggaggc aataactacg ccgcaaaagt
    10021 gaatgaccag aaaatcagcc gtgggcaatt cgagaacgcc ttcaacagcg agcgtaatcg
    10081 catgcagcaa cagctgggcg atcaatactc cgagctggca gcgaacgaag gctatatgaa
    10141 aaccctgcgt caacaggtgc tgaatcgtct gatcgacgag gcgctgctgg atcagtacgc
    10201 acgtgagctg aaactgggta tcagcgatga gcaggttaaa caggcgattt tcgcgacccc
    10261 agccttccag gttgacggca aatttgataa cagccgctat aacggtatcc tcaaccagat
    10321 ggggatgacc gccgatcagt acgcccaggc gctgcgtaac cagctcacta cccaacagct
    10381 gattaacggc gttgccggta ccgattttat gctgaaaggt gaaaccgacg agctggcggc
    10441 actggtcgcg caacaacgcg tggtgcgtga ggcgactatc gatgttaacg cgctggcggc
    10501 gaagcagcct gtgaccgaac aggagattgc cagctactac gaacaaaaca aaaacaattt
    10561 catgacgccg gaacaattcc gcgtgagtta catcaagctg gatgccgcaa cgatgcagca
    10621 accggttagc gatgcggata tccagagcta ctacgaccag catcaggatc aattcaccca
    10681 gccgcagcgt acccgctaca gcatcatcca gaccaaaact gaagatgaag cgaaagcggt
    10741 acttgatgag ctgaataaag gcggtgattt tgctgcatta gccaaagaaa aatctgccga
    10801 tattatctct gctcgtaacg gcggcgatat gggttggtta gaagatgcca ctatcccgga
    10861 cgaactgaaa aatgctggtc tgaaagaaaa aggccaactg tctggtgtca tcaaatcttc
    10921 ggtcggtttc ctgattgtac gtctggacga cattcagcca gcgaaagtga aatcgttaga
    10981 cgaagtacgt gacgacattg cggcgaaagt gaaacacgaa aaagccctcg atgcgtacta
    11041 cgcgctgcag cagaaagtga gcgatgcggc aagcaacgac accgagtctc tggccggtgc
    11101 agagcaagct gccggcgtta aagccactca gacgggttgg ttcagcaaag ataacctgcc
    11161 ggaagagttg aacttcaagc cggttgccga cgctatcttt aacggcggtc tggtaggtga
    11221 aaacggcgcg ccgggcatca actctgacat catcaccgta gacggcgacc gcgcattcgt
    11281 gctgcgcatc agcgagcaca aaccggaagc ggtgaaaccg ttggcagatg ttcaggaaca
    11341 agttaaggca ctggttcagc acaacaaagc tgaacaacag gcgaaagtgg atgctgagaa
    11401 actgctggtt gatttgaaag ccggcaaagg tgcggaagct atgcaggctg ccggtctgaa
    11461 atttggcgag ccgaaaacct taagccgttc cggtcgtgac ccgattagcc aggcggcgtt
    11521 tgcactgcca ctgccagcga aagacaaacc gagctacggt atggcgaccg atatgcaagg
    11581 taatgtggtt ctgctggcgc tggatgaagt gaaacaaggt tcaatgccgg aagatcagaa
    11641 aaaagcgatg gtgcagggta tcacccagaa caacgcacaa atcgtctttg aagctctgat
    11701 gagtaacctg cgtaaagagg cgaaaatcaa aattggcgat gcgctggaac agcaataatc
    11761 ctgaagccgc ctcgcaaaaa aatgcgttgc agctgtaaca agaaaaggtc gctttcgcgg
    11821 ccttttccat ttctgaacat tgccatttgt ttactgtttt cactgccgtt aaggtgattc
    11881 cactgttaac aaacaaggag aaaacagtat gaaacacgga attaaagcac tgctcattac
    11941 cctgtccctg gcctgtgccg gaatgtctca tagcgcgctg gcggcagctt ctgtggcgaa
    12001 accgacaacg gtagaaacca aagcggaagc tcctgcagca caaagtaaag cagcagtacc
    12061 ggcgaaagcc agtgacgaag aaggcacccg ggtcagcatt aataatgcca gcgcggaaga
    12121 gctagcccgc gcgatgaatg gcgttggcct gaagaaagcg caggcgattg tcagttatcg
    12181 cgaagagtac ggtccgttta aaacggtgga ggatctaaag caggtgccgg ggatgggcaa
    12241 ttcgctggtg gaacgtaatc tggcggtatt aaccctgtaa ttaatttgca tagtggcaat
    12301 tttttgccag actgaagagg tcataccagt tatgacctct gtacttataa caacaacgta
    12361 aggttattgc gctatgcaaa cacaaatcaa agttcgtgga tatcatctcg acgtttacca
    12421 gcacgtcaac aacgcccgct accttgaatt tctcgaagaa gcccgctggg atgggttgga
    12481 aaatagcgac agttttcagt ggatgacggc ccataacatc gccttcgtcg tggtcaatat
    12541 caatattaac tatcgtcgcc cagcggtatt aagtgacctg ttaactatta ccagtcagtt
    12601 gcagcaatta aacggtaaaa gcggcatctt aagccaggtc attacactgg agccggaagg
    12661 gcaggtggta gcggatgcgc ttattacgtt tgtttgtatt gatcttaaaa cgcagaaagc
    12721 attagctctg gaaggggaat tgcgcgaaaa gctggagcag atggttaagt aaacgttttg
    12781 tggtgccgga tgctcaagcc gcatccggcg acacccggaa taattacctc aacccggttt
    12841 tctgcttcat cgctgccatc accgtcggtt tatcggccag ataatgattc aaaccgttgg
    12901 cgcgtaaatt acatgccgca caatgaccgc aaccgtcgcc tttaatgccg ttatagcagg
    12961 tcaacgtttc gttacggact aaatccagtt tgccgtaata atctgccagc gcccaggttt
    13021 ccgctttatc aatccacatc agcggcgttt caaaacgaat atctttcgcc atgcccaaac
    13081 tgacggcatg gtttagtgct ttcacaaact catcgcggca atccgggtag ccggagaaat
    13141 ccgtttcaca gacgccggta attacggctt ctgcttttac ctgatacgca tatattgccg
    13201 ccagcgtcag gaacaaaata ttacgccctg ggacaaacgt attcgggatg ccatcggctt
    13261 caggttcata atcaggcacc ggaatgctgt cacgtgtcag gctgctgacc gccagctcgt
    13321 tgagcagcgt tacatcaaga accttatgcg cgcgtgctcc cagtttcagc gccagttcgc
    13381 gtgccacgtc gatttctgcg cgatgtcgct gaccgtaatc gaacgtcacg caatggactt
    13441 catcatattg ttgtaatgcc tgcaccagac aggtggtgga atcctggcct ccactgaaca
    13501 caacgacagc acgtttcata gataatcctg cttgaacaat aaaagcgtta tggtaacgcc
    13561 tgcgattaac ccggaccagc tattcattgc gacggcgcgg gaagccaggc ttcggtaaat
    13621 tcaaaccagc cgcgcggtgt cagtcgcaca ccgttcacgc cgggaggggc actaatgcga
    13681 tagtgatagt tgaacagcgg cgtcagcgtc gcgtctgcca ttaactggct aaaaaccgct
    13741 ttcagggcat taaatcggtt ttcctcatca ggcattactt gcaccgcgtc cagcgtcgat
    13801 tgcagatgtg catatgctgg agcgtcgaaa acatgtggcc acagtggatc gcaacgtagc
    13861 cattgctcca gagtatattc cggtgcttcg ccaattaatc tgtcgcccat catgaggtct
    13921 gcgtgtgcca gtagggtcgt gtcgtcccag ttttttgcgt tatgaaaaat aattgtgagt
    13981 tcacagcctt ccgctgacag tgtcgcctgt aggcgttctg ccatggtatg aagttctatc
    14041 ggtaggtgat aaaccagcgt caaggttttc ggtagtttga cttcatccgg tacttgccag
    14101 tgcggaatag tccagcctgg cagtaatgca tgactggcgg tgatcaggtt ttctccgact
    14161 tctaacgttt gtaataaacc ggattgatga ataatggaga tcacttttcg cgcctgccag
    14221 agggagagtc ggggactttt gcgcaacgtt aaatagcaaa aacctaaact gatgccacta
    14281 ctgacctggc tgacccgttg cagctcctcc ggtttgccga tggtgatttg cacgggatgc
    14341 cgacaactgg ttcccaaatc tttttcgaaa agcggcggag ttatccagta ctcaaccgct
    14401 ttaagcagcg gatgacgcaa atggtaataa tcatggctct ccaggcgcac cagctccgct
    14461 gtgaattgtg ttaagcgaaa aggaccagtg ccgatcagtg ggaattgcgg atgcaccaga
    14521 tggctgcaat agctcgccag ccggtgcgca agccagtaat cggggcgatg taaaaagaag
    14581 gtcagacact gcggatgggt gacttcaata cgcttcacgc taataaataa ttgatccagt
    14641 gctggcagtt gtaacagcat caataatcgc tggtgtaagt gtgaggcttt tactgcatcg
    14701 ccgttatgcc agtgtagggt tgaacgaaga taaaagtccc agcgtaaccc gtcagtagag
    14761 gtttcccagt gatgcgctaa atcgccaatc gggcgctgag tattattatc gaagcgggtc
    14821 aggccggaaa atatctgccc ggcgagatgc tgctcggcac ggccgggcaa aaagcctggt
    14881 tgtagcggtt cgagcgggcg atagtaggga atacgcaacg tgggtgtatc gttttgccat
    14941 tgtccgccca taaacggctg taacagagtg cgcagctcac ctggggccag ttgcgccagc
    15001 tccagcacat cttgctgctt tccggtttcc agtgcctgtt ccatcatcgc attgcgtagc
    15061 gattccggcg tgaccagaaa gcgtaattgt ccgcgctttc cgcgtcctga ctgcgcctgc
    15121 cactccagcc atcccgcctc ctgtgcctga cgcaacagcg tacgaacatg gcgttcgctg
    15181 caaaaacagc gttcggccag ttcgctgacg gtgacggttt gcggctttcc ggctgaaggt
    15241 tgccacagac gttgatactg gttaagacgg ttgagcaatc gcatataaac ccggaacaat
    15301 attatttcac tattcactat tacttccgta tatatcaggt gatactcaat caccattaac
    15361 cgtgtcacag agtggagaaa gaaatggctc gtctggcagc atttgatatg gatggcactt
    15421 tattgatgcc tgaccatcat ttaggtgaga aaaccctctc tactttggcg cgactgcgtg
    15481 aacgcgacat taccctcact tttgccacgg ggcgtcatgc gctggagatg cagcatattc
    15541 tcggggcgct atcgctggat gcatatttga ttaccggcaa cggaacgcgc gtgcattctc
    15601 tggaaggtga acttttacat cgtgatgatt tacctgcgga tgtcgcggag ctggtgctgt
    15661 atcagcaatg ggatacccga gccagcatgc atatcttcaa tgacgacggt tggtttaccg
    15721 ggaaagagat ccctgcgttg ttgcaggcat ttgtctatag cggttttcgt tatcagataa
    15781 tcgatgtcaa aaaaatgcca ctcggcagcg tcaccaagat ctgcttctgt ggcgatcacg
    15841 acgatcttac acgcttgcag atccagctat acgaagcatt aggcgagcgt gcacatttgt
    15901 gtttttccgc cacggattgc ctcgaagtgc tgccggtggg ctgcaataaa ggcgctgcat
    15961 tgacggtgct gacccaacat ttaggtttat cgttgcgcga ttgcatggcc tttggtgatg
    16021 cgatgaacga tcgcgaaatg ttaggcagcg tcggtagcgg gtttattatg ggcaatgcga
    16081 tgccgcaact gcgcgcggag ctcccgcatt taccggtgat tggacattgc cgaaatcagg
    16141 ctgtctctca ctatttgacg cactggctgg actatccaca tctaccttat tcccccgaat
    16201 aatgagatcc cttccagcac cgggcaattg cccggttttt tttgcgttga atttgtcatt
    16261 ttgtgccgtg gtgtttaaac cgcacagaat aaattgtcgt gatttcacct ttaaaataga
    16321 attaaaagag aaaaaaattc tctgtggaag ggctatgtta gataaaattg accgtaagct
    16381 gctggcctta ctgcagcagg attgcaccct ctctttgcag gcactggctg aagccgttaa
    16441 tctgacaacc accccttgct ggaagcgcct gaaacggctg gaggacgacg gtatccttat
    16501 cggcaaagtc gccctgctgg atccggaaaa aataggcctc ggcctgaccg cttttgtgct
    16561 gataaaaacg caacatcaca gcagcgaatg gtattgccgc tttgtcacgg tggttaccga
    16621 aatgccagaa gtgctggggt tctggcgcat ggccggtgaa tacgattatc tgatgcgcgt
    16681 ccaggttgcc gacatgaaac gctacgacga gttttataag cgtctggtaa acagcgtgcc
    16741 ggggctgtcg gacgtcactt ccagcttcgc gatggaacag attaaataca ccacttcttt
    16801 acccatcgaa taaatatcca gaatcaggtc aggacacaac gcgtgcgatt atttgctcaa
    16861 ttaagctggt atttccgtcg ggaatggcgt cgctatctcg gggctgtcgc cttgcttgtc
    16921 attatcgcga tgctgcaact ggttccacca aaagtggttg gtattgttgt cgatggcgtg
    16981 acagaacaac actttactac cgggcagatc ctgatgtgga tcgccaccat ggtgctgatt
    17041 gccgttgtgg tttatctcct gcgttacgtc tggcgcgtat tgctgtttgg tgcgtcttat
    17101 caactggctg ttgaactgcg tgaagattat taccgtcagc taagccggca gcatcctgag
    17161 ttttacctgc gtcatcgcac gggtgacctc atggctcgtg cgactaatga cgtcgatcgc
    17221 gtcgtgtttg ccgccggaga aggggtgctg acgctggtgg attcactggt aatgggctgc
    17281 gctgtgttga ttatgatgtc tacgcaaatt agctggcagt tgaccttatt tgccctgtta
    17341 ccgatgccag tgatggcgat catgattaag cgcaacggcg atgctttgca tgaacgcttt
    17401 aagctggcac aggcggcgtt ttccagtctt aatgaccgca cccaggaaag cctcaccagt
    17461 atccgcatga tcaaagcctt tggtctggaa gatcgccagt cggcgttatt tgccgcggat
    17521 gccgaagata ccggcaaaaa aaacatgcgg gtggcgcgta ttgatgctcg tttcgacccg
    17581 accatctata tcgcgattgg tatggcgaac ttgctggcga ttggcggcgg tagttggatg
    17641 gtggtgcagg gcagtttaac gctgggccag ctcaccagtt ttatgatgta tttaggtctg
    17701 atgatttggc caatgctggc gctggcatgg atgtttaaca ttgtggaacg tggtagtgct
    17761 gcgtacagcc gtattcgcgc gatgctggcg gaagcgccgg tggtgaacga tggtagcgaa
    17821 cccgtgccgg aagggcgtgg cgaactggat gtaaatattc accagttcac gtatccgcag
    17881 actgaccatc ctgcgctgga aaacgttaat ttcgccctga aacccggtca gatgctgggt
    17941 atctgcgggc cgactggttc cggcaaaagt accctgttgt cgctcattca gcgtcatttc
    18001 gacgtcagcg agggggatat tcgctttcat gatattcctc tgacgaagtt acaactcgat
    18061 agctggcgta gccgcctggc ggtagttagc cagacgccat tccttttttc tgacactgtg
    18121 gcgaataaca tcgcgctggg ttgcccgaat gccacccagc aagagattga gcatgtcgcg
    18181 cggttagcca gcgtacatga cgatattttg cgtctaccgc aaggttacga tacagaggtg
    18241 ggcgagcgcg gtgtgatgct ttccggcggg caaaaacagc gtatctccat tgctcgtgcg
    18301 ttattagtca acgcggaaat cctcatcctt gatgatgcgc tttcggcggt ggacggacgc
    18361 actgagcacc agatcctgca taacctgcgt cagtgggggc agggaagaac ggtaatcatc
    18421 agtgcccatc gcctttctgc actgacggaa gccagtgaaa ttattgtgat gcagcacgga
    18481 catatcgccc agcgtggcaa tcatgatgtg ctggcacaac aaagcggctg gtatcgcgat
    18541 atgtatcgct atcaacaact ggaggcggcg ctcgacgacg ctccggaaaa tcgcgaggag
    18601 gccgtcgatg cgtagtttta gccaactgtg gccgactctc aagcgcctgt tagcgtacgg
    18661 ttcaccgtgg cgtaaaccgc tggggattgc ggtcctgatg atgtgggttg cggcggcggc
    18721 agaagtcagt gggccgctgc ttatcagcta ttttatcgac aatatggtag cgaaaaataa
    18781 cctgccgttg aaagtggttg cagggctggc tgcggcgtat gttgggctgc aactgtttgc
    18841 cgccgggcta cattacgcgc agtcgctgct gtttaatcgg gcggcagtag gcgtagtgca
    18901 acagttgcgt accgacgtga tggatgctgc gttacgccag ccattaagcg agtttgatac
    18961 ccaacccgtc gggcaggtga tttcccgcgt cactaatgac actgaagtga tccgcgatct
    19021 ctacgttacc gtagtggcaa ctgtcctgcg cagtgccgcg ctggtgggcg cgatgctggt
    19081 ggcgatgttc agcctcgact ggcgaatggc actggtggcg ataatgattt tcccggtggt
    19141 gctggtggta atggtgatat accagcgtta cagcacgccg attgtccgtc gtgtgcgcgc
    19201 ctatctggcg gatatcaacg acggctttaa cgaaatcatc aatggcatga gcgttatcca
    19261 gcagtttcgt cagcaggcgc gatttggcga acgtatgggg gaggccagtc gttcacacta
    19321 tatggcgagg atgcaaaccc tgcgcctcga cggttttctg ctgcgtccgc tgctgagtct
    19381 gttttcatcg ctcattcttt gtggcttgtt gatgctgttt ggcttctccg ccagcggcac
    19441 cattgaagtg ggcgtgctgt atgcgtttat cagctatctt gggcgactta acgaaccatt
    19501 aatcgaactg accacgcaac aggcgatgct gcaacaggct gttgttgctg gtgagcgcgt
    19561 gtttgaactg atggacggac cgcgccagca atatggcaat gatgatcgcc cgttacagag
    19621 tggcaccatc gaagtcgata acgtgtcatt tgcttatcgc gatgacaatc tggtgctaaa
    19681 gaacattaat ctctctgtgc cttcgcgcaa ttttgtggcg ctggtcgggc ataccggcag
    19741 tggcaaaagc accctcgcca gtttattgat gggctattac ccgctaacgg aaggtgagat
    19801 tcgccttgat ggtcgtccat taagttcgct aagtcacagc gcgctgcgcc agggcgtggc
    19861 aatggtgcag caagatccgg tggtgctggc ggataccttc ctcgccaacg tgacgctggg
    19921 gcgggatatc tccgaagaac gcgtctggca ggcgctggaa accgtgcaac tggcggagct
    19981 ggcgcgtagc atgagcgacg gtatttacac gccgctgggc gagcagggga ataatctctc
    20041 agttgggcaa aagcaactgc tggcactggc gcgcgtgctg gtcgagacgc cgcaaatcct
    20101 gatccttgat gaggcaaccg ccagcattga ctccggtact gaacaggcga ttcaacatgc
    20161 tctggcggcg gtgcgtgaac ataccacgct ggtagtgatt gctcaccgct tatcgaccat
    20221 tgttgatgcc gacaccattc tggtgcttca tcgtgggcaa gccgtggagc agggcactca
    20281 ccagcaactg ctggcggccc agggacgcta ctggcagatg tatcaactgc aacttgcggg
    20341 cgaagagctg gcagccagcg tgcgtgaaga ggaatcattg agcgcctgaa tagcgcaata
    20401 tttcatcgtt ggtgcaaaaa tgtaacgcac tgtgcactgt catagtgcgt tttcattttc
    20461 aaacttctta acttcctgct ctctttctcg tttttcattt ctggcacacc gcttgcaata
    20521 ccttcttcgt gtagcagaac cattaccgaa ttctgaccgg aggggatcta tgaagctggt
    20581 gaccgtgata atcaaaccat tcaagctgga agacgttcgt gaagcgttat cttccattgg
    20641 tattcagggc ctgaccgtca ccgaagtgaa aggtttcggg cgtcagaaag ggcatgccga
    20701 gctgtaccgg ggggcggaat acagcgtcaa tttcctgcca aaagtaaaaa ttgatgtggc
    20761 gattgctgat gaccaactcg atgaagtgat cgatatcgtc agtaaggcgg cttacaccgg
    20821 aaaaattggc gacggcaaaa tcttcgtcgc tgaattgcaa cgcgtcattc gtattcgtac
    20881 cggcgaagcc gacgaagcgg cgctgtaatc tctggcacac agcaacagga acgaaaaatg
    20941 aagatagcga cgataaaaac tgggcttgct tcactggcga tgcttccggg actggtaatg
    21001 gctgcacctg cggtggccga taaagccgac aatgcgttta tgatgatttg tactgcgctg
    21061 gtgctgttta tgactattcc ggggattgcc ctgttttacg gtgggttgat tcgcggcaaa
    21121 aacgtgctgt cgatgctgac gcaggtgacg gtgacatttg cactggtctg tattctctgg
    21181 gtggtttacg gttactcgct ggcgtttggt gagggcaaca acttcttcgg caacattaac
    21241 tggttgatgc tgaaaaacat agaactgacg gcggtgatgg gcagcattta tcagtatatc
    21301 cacgtggcgt ttcagggatc gtttgcctgc attaccgtcg gcttgatagt tggggcgctg
    21361 gcggaacgaa tccgcttctc agctgtgttg attttcgtgg tggtatggct gacgctctct
    21421 tacattccga ttgcgcatat ggtgtggggc ggtggtttgc tggcttctca cggtgcgctg
    21481 gatttcgcgg gtggcaccgt ggtgcacatt aacgccgcaa tcgccggtct ggtgggcgcg
    21541 tatctgatag gaaaacgcgt gggcttcggt aaagaggcgt ttaaaccgca caacctgccg
    21601 atggtcttca ccgggactgc cattctctat atcggttggt ttggctttaa cgccgggtca
    21661 gcgggcacgg cgaatgaaat cgcggcactg gcatttgtga atactgtggt cgcaacggcg
    21721 gcggcaattc ttggctggat cttcggtgaa tgggcgctgc gtggtaagcc ttcactgctg
    21781 ggggcgtgtt ctggcgcgat tgccggtctg gtcggcgtga cgccagcctg cggctacatt
    21841 ggggttggcg gcgcgttgat tatcggcgtg gtagctggtc tggcgggctt gtggggcgtt
    21901 accatgctca aacgcttgct gcgggtggat gatccctgcg atgtcttcgg tgtgcacggc
    21961 gtttgtggca ttgtcggctg tatcatgacc gggatttttg ccgccagctc gctgggcggc
    22021 gtgggcttcg ctgaagatgt gacgatgggc catcagttgc tggtacagct ggaaagcatc
    22081 gccattacga tcgtctggtc cggtgttgtg gcatttatcg gctacaaatt ggcggatctg
    22141 acggttggtc tgcgtgtacc ggaagagcag gagcgagaag ggctggatgt caacagccac
    22201 ggcgagaatg cctataacgc gtaacaagca ctgcaaaaaa cagccggacg gttttcacct
    22261 ccggctattt ttttaattgt gattacgcat caccccttcc tgaacggtcg aggcaaccag
    22321 tacgccgtct tgggtataaa actcaccgcg cacaaagcca cgtgcgctgg acgccgaggt
    22381 gctctccacg ctatatagca gccattcatt caaattaaac gggcgatgga accacatgga
    22441 atggtcaatg gtggcaatct gaatccccgg ttcgagaaaa ccgatgccgt gcggctgtag
    22501 agctaccggc aggaagttaa gatcagaagc gtaaccgagc agatactgat gaacgcgcag
    22561 gtcatccggc acgctaccat ttgcgcggat ccacacctga cgatgtggtt ctgcgacgtg
    22621 acctttcagt gggttatgaa actccaccgg acggacttcc agcggacgat cgcagatgaa
    22681 tttatctttc agcactggcg gcagcaggtg cgccagcgat tgggcgattt gcgtttccga
    22741 aggtaggcca tcaggcgctg gcgcggacgg cattgttttt tgatgttcga aacccgcttc
    22801 tggtgcctgg aaagaggcag tcatataaaa aatcggtttg ccgttttgaa tagcagcaac
    22861 ccggcgggcg ctgaagctgt taccgtcacg cagcgtttcg acatcataaa taatcggctt
    22921 cttactatcg ccagggcgaa gaaagtagct gtgaaacgaa tgtaccagcc gctcttcagg
    22981 gacggtctct tttgcagcat acaaggcctg acccacgacc tggccgccaa acacctggcg
    23041 taaacctaaa tcttcactct ggccgcgaaa gagtccttcc tcaatttttt ccagatttaa
    23101 caatgtcagt aaatttttta gcgcctgact catataactc tccagtaaca aagctgccgc
    23161 agcaagccaa agtgagttga gtataacgca aatttgctac tggtccgatg ggtgcaatgg
    23221 tctgaattac gggctaatta caggcagaaa tgcgtgatgt gtgccacact tgttgacgtt
    23281 actattttgt taaccactct tccggcgagg aaagttagcc cgctggtgca ttgataataa
    23341 ggagaaatga atgaaactcg tgcacatggc cagtggttta gcggttgcga ttgcgttggc
    23401 ggcttgcgca gataaaagcg cggatattca gacgccagca ccggctgcaa atacgtctat
    23461 ttcagcaaca caacaaccag ctatccagca accgaatgtc tccggtaccg tctggatccg
    23521 tcagaaagtc gcactgccgc ctgatgctgt gctgaccgtg acactttctg acgcgtcgtt
    23581 agccgatgca ccgtcaaaag tgctggcgca gaaagcggtg cgtactgaag gtaaacagtc
    23641 accattcagc tttgttctgc catttaaccc ggcagatgtt cagccgaacg cgcgtattct
    23701 gttgagtgcg gcgattaccg tgaatgacaa actggtattt atcaccgata ccgttcagcc
    23761 ggtgatcaac cagggcggaa ctaaagccga cctgacattg gtgccggtac agcaaaccgc
    23821 cgtgccggtt caggccagcg gtggcgcaac gactaccgta ccttcgactt caccaactca
    23881 ggtgaatccg tcttcggcag ttccagctcc tacgcaatat taagcgacgt taaccctctc
    23941 cgcccggaga gggttagtag ttccagcgat aacgctgcaa gtcgatttgc ccgcttcccg
    24001 ataccatcac accttctgcc agtaatgcct gtcgctgacg ctgtaaatcc ggtccggtta
    24061 gcgaaattgt gccgtggcga ttaaccaccc ggtgccaggg taaggtgctg ccttcaggga
    24121 gacgctttaa cacaccgccc acctggcgcg cggcgcgggg cgatcccgcc agtttcgcca
    24181 catcaccgta agtggtgaca tagccttcgg gaatagcggc gacgatttgc cagacgcgtt
    24241 ggggaaatga atcttctttt tccatctttt cttcctgagg taatttttca gcataatctg
    24301 gaaaaacgcc cgagtgaagt cgcattgcgc aagaaaccag catctggcac gcgatgggtt
    24361 gcaattagcc ggggcagcag tgataatgcg cctgcgcgtt ggttctcaac gctctcaatg
    24421 ggggctctgt tggttctccc gcaacgctac tctgtttacc aggtcaggtc cggaaggaag
    24481 cagccaaggc agatgacgcg tgtgccggga tgtagctggc agggccccca cccatttctg
    24541 cctcccaccg tttcgtcaaa aaatcccaac atggctaaac tttaaccaca actgacgtcg
    24601 caagaattgt ctggctgcgc agtacgcttc ggaggtatgt ctgatgaagt atgttgatgg
    24661 ttttgtggtt gccgttcctg ccgataaaaa ggatgcctat cgggaaatgg ccgctaaggc
    24721 tgcgccattg tttaaagagt ttggcgcgct tcgtattgtc gaatgctggg ccagcgatgt
    24781 accggatggc aaagtgaccg attttcgtat ggcggtgaaa gcggaagaga atgaagaggt
    24841 tgtctttagc tggattgaat acccttcaaa agaggtccgc gacgctgcta atcaaaagat
    24901 gatgtcggac ccacggatga aagagttcgg cgagtccatg ccgtttgacg gcaagcgaat
    24961 gatctatggc ggattcgagt caatcatcga cgaataggta gcgtgacggg ctgtgcggcg
    25021 tgccgtcagc ccaaaaattc acaaatgttg ctcggcccag cgtaagaaat cttctttcgg
    25081 taatgccttg ctgtagagcc agccctgacc gtagtgcacg ccatgctggc gtaaccactc
    25141 ttcttgttta ctggtttcga ttccctccgc taccattttc agtttcagtg tttttgccat
    25201 ttcgatgata tgcggcgtga catttttata ttccagcgca tcaacgaaag atttatcgat
    25261 cttcagaatg tcgacatcca gatcctgtaa ataacttaaa cttgaatacc ccgtaccaaa
    25321 atcatcaaga taaatttcat ggcccgcctc ccggtagcga gaaattatcg gggcgctggt
    25381 tttcggatcg gcaaactcgc gttcagtgag ttcaagcgcg atctgtctgg gattaacctg
    25441 atagtgattg atcatgtcac gcagcaattg cgggattttt tccgaggtga gcacggggga
    25501 ttcaagattg atcgaaatat gctgctgtgg atgctgacgc agccagtcgc ccatatcttc
    25561 aaagacgctt cttataatca gtagcgtcaa tggctcagaa aggcccgttt gctgtgccag
    25621 cggaataaaa ctatctggtg acaaccaact accgtctgtc tgcggccagc gcgccagtgc
    25681 ctcagcaccg acaattttgc cattggctaa ggagacaatc ggctgatagt gcacgcaaat
    25741 atcacgattt tcgatagcat cctgcagccg atgatgcggt gactgaatac ggcgcaaaat
    25801 acgcagcaca aacatcgctg ccagcaggcc aatcaccaac ccgagcggta accaaataaa
    25861 gacttgccga tgccagcctt tctgcaacat tttcgttgaa gcccatgtga tgatcgaaat
    25921 attcatctcc ggtaagggca ggatatcgta aatgattcca ttattttcga tatgctcacc
    25981 gggtgttttt tgtagcctgg taataattcc ctgagcaatt tcatcgctgc tggttatgac
    26041 aacgttatgg gcattgccaa taatggcggc atcaatttgc catgagctat agggaatgac
    26101 atcaataaag gaagcggggt cgatcatgac gacataatgt gccgttccca tggcgaccat
    26161 gtaacggata atgcctaaat cgttatgcga tgttaaccag acacgataac catctttcga
    26221 aattttacct ggctcgggga aggtatcggg cggactttca tgctccagag acgaacactg
    26281 gggaacgttg ttatcgatat aagccacttc ctgaatatag cgataactgt aagatacccg
    26341 acgcatttcc atgagatggg cttcgctaca ggcagcgcct tgccatcttt ccagctcctg
    26401 cagcgcatct ttcccttgtg tcgccacctt attggctcga atagcgacgc gggaggaata
    26461 ggtatccagc tcttcaataa acgatgtttc tacctgctga tgggccagcc agatgcttaa
    26521 gccgacaggc agcaatactg aaagaatcag tactcccgaa ataaggccga ccagatgtcg
    26581 tgttctcacc agcatatcct tattaacaca gtgtagggaa agtatatctg attgtggaaa
    26641 aaggctggct ggagataagg aggtagagct ggtatttcat gaagtgatgg gtggaaacga
    26701 tcattgtcat ttgaccaggc gcagagcttt acgcctggta tgtaacccta acttaagatg
    26761 ttgattcggc gcgagcgagg gtaaaaatat cgtaaaaaga cagttcacct ttacggacga
    26821 tcattttttg cagttgttcc ttatgaacat ccgcgacacg cggagagttc aagatggctt
    26881 caaccagcgc ggcaggtaca aagcgcgtgt tgtaccattc gccgttatag caaacgcgga
    26941 aatccagcac gtcgatatct tcatagcggt agcgtgggta gacatcgaaa aagaagaaac
    27001 cattaagcag gataaataat acaaccagta gccagacaga gcctgacagc gtttcggact
    27061 gcaacatcac cgccagggtg gcaaaaaaag caacgtacat accgataaac agccccggat
    27121 gtttacggat aaaactgata ctaaagcggg gtttgttgtc gcgtttttcg cgggtattca
    27181 gagactcaat cgtttcggtc agcaggcgtt gtatttcggt catcgtttgc cttcgtggta
    27241 tctgcaactt tccagggaac accctatttt aacgtgggaa tagtagaggg gaagtaacag
    27301 atctaccaca attttgcata acagttgcga aaaaccgatt acaatttttt aattattctg
    27361 gctggattac cgcccacgac aacgttgtcc gggacatctt ttgtgacaac tgcacctgag
    27421 gctaccacga cgttatcacc aatggtcaca ccagggttaa tgaccgcgcg tccgccaatc
    27481 cagacgttat taccgatggt gacgggtttc cccagttcag caccgctatt acgtgctaca
    27541 gggtcgatgg gatgtgttgc cgtgtagata tgaacgcctg gtgccaacat acagttatca
    27601 ccgatgcgaa tagggcagac atcaagcatc acgcaatcga agttggcgaa aaaattatta
    27661 ccgagaaaaa tgttatagcc atagtcacag cgaaacgttg gctcaatata agcctctgtc
    27721 acctgaccga atagatcagc gagaatttgc tggcgtaatg tgtgctcttc cgccagggaa
    27781 tgattgtatc ggtgaataag ctgacgagcg cgcaggcgat cgcgagataa cgtctcatct
    27841 gccgagcgat acaactcacc agcaatcatc ttttcttttt ctgtgctcat tactgacctc
    27901 cggttgctga aaatgcggca acacagtaag acgaaaagtc agtgaagaga acgttcccat
    27961 tacatctgtg tgataaagat cacatagggt atgttttcat ggcgaaaaag tataaaattc
    28021 ttaataaaca gccggttata gctccgaaag cgaattattt attagcgaat aaatttccaa
    28081 actgaggaag ggatcttgtc gtacagttta ttcatggtca attcggcgag gcggtgatct
    28141 gcggctgagt aaaataccgc cagttcatta tctgataatt cgtatttatt tttctcgata
    28201 acacgctcca gcgtgtcaat tgtctggcaa cgacgtaaac gcattaaata atcggttttc
    28261 gttaaaggtt tttcggacat acttctaccc atggttgtaa taattctaac aagataaact
    28321 cgcaggattc tctttcgtcg cattgacaaa acaacggaat agtcgattac ctgacttccg
    28381 ccatttctga agatcctgca tattaatacc ataactactg aacaacataa aggtgtcatc
    28441 cagatattcg tcgatctgct caatgagctt attgtcttca ttatacttaa ttttgtaatt
    28501 aagtgcgaag gtcgcaatat gctcaatcag ttcattcaac tgtaggttga tcgccgaggt
    28561 tgggtcgttt acccagccat gattgctttc ttcaaggttt gcaaggcagt catgatacag
    28621 ggtttcacag agaaacttaa gctgtgcgat atcatgtctt ttgggtgagt attcatccat
    28681 aacgcgtccc cttcttagcg gttgaactaa cggacacctt tcgggatgga aaaaacttac
    28741 tgacctggac ttgccctctc gtttgttagc ttaactataa gccactcttt gcaggttttc
    28801 atcgcattat tacgaaaaat tacaattagg aaggaagcgg gacaggactc actttttcat
    28861 actagcacta acccgcagca ggtaaaagca gtttgctcga actattcata acatttcagg
    28921 attatcgcca gatgaaattg gtgacactaa taccaggatt gctctgaata tgacgtaata
    28981 accgaggaat gaataaagaa ttaccgcaaa taattaagaa tagcctctaa atgattatgg
    29041 attattaaat
//
LOCUS       DAPHBJ010000038        28805 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.038, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000038 DAPHBJ010000000
VERSION     DAPHBJ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.038"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            201..971
                     /locus_tag="RHZ76_001523"
     CDS             201..971
                     /locus_tag="RHZ76_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDV2812182.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSDTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            962..1435
                     /locus_tag="RHZ76_001524"
     CDS             962..1435
                     /locus_tag="RHZ76_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HDV2812183.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1542..2081
                     /gene="dnaT"
                     /locus_tag="RHZ76_001525"
     CDS             1542..2081
                     /gene="dnaT"
                     /locus_tag="RHZ76_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HDV2812184.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2084..2821
                     /gene="dnaC"
                     /locus_tag="RHZ76_001526"
     CDS             2084..2821
                     /gene="dnaC"
                     /locus_tag="RHZ76_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HDV2812185.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2870..3364
                     /locus_tag="RHZ76_001527"
     CDS             2870..3364
                     /locus_tag="RHZ76_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HDV2812186.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3618..5909
                     /gene="opgB"
                     /locus_tag="RHZ76_001528"
     CDS             3618..5909
                     /gene="opgB"
                     /locus_tag="RHZ76_001528"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HDV2812187.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGAGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6050..7072)
                     /gene="lgoD"
                     /locus_tag="RHZ76_001529"
     CDS             complement(6050..7072)
                     /gene="lgoD"
                     /locus_tag="RHZ76_001529"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HDV2812188.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7211..8125
                     /locus_tag="RHZ76_001530"
     CDS             7211..8125
                     /locus_tag="RHZ76_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV2812189.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENMRYAH"
     gene            8342..9703
                     /locus_tag="RHZ76_001531"
     CDS             8342..9703
                     /locus_tag="RHZ76_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2812190.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9752..11416)
                     /gene="tsr"
                     /locus_tag="RHZ76_001532"
     CDS             complement(9752..11416)
                     /gene="tsr"
                     /locus_tag="RHZ76_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HDV2812191.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLHDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKNVTPATPRKMAVADSGENWETF"
     gene            11793..13943
                     /gene="btsT"
                     /locus_tag="RHZ76_001533"
     CDS             11793..13943
                     /gene="btsT"
                     /locus_tag="RHZ76_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HDV2812192.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFISALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13993..14196
                     /locus_tag="RHZ76_001534"
     CDS             13993..14196
                     /locus_tag="RHZ76_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDV2812193.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            14207..15163
                     /gene="yjiA"
                     /locus_tag="RHZ76_001535"
     CDS             14207..15163
                     /gene="yjiA"
                     /locus_tag="RHZ76_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HDV2812194.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(15197..15499)
                     /locus_tag="RHZ76_001536"
     CDS             complement(15197..15499)
                     /locus_tag="RHZ76_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HDV2812195.1"
                     /translation="MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDE
                     QWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKGYQTRLNAILREAMLRDQNKK"
     gene            complement(15486..15773)
                     /locus_tag="RHZ76_001537"
     CDS             complement(15486..15773)
                     /locus_tag="RHZ76_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDV2812196.1"
                     /translation="MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIEN
                     GEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIISARKADRKERNRYEHG"
     gene            complement(15972..16669)
                     /locus_tag="RHZ76_001538"
                     /pseudo
     CDS             complement(15972..16669)
                     /locus_tag="RHZ76_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            16726..16821
                     /locus_tag="RHZ76_001539"
                     /pseudo
     CDS             16726..16821
                     /locus_tag="RHZ76_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
     gene            16967..18199
                     /gene="mdtM"
                     /locus_tag="RHZ76_001540"
     CDS             16967..18199
                     /gene="mdtM"
                     /locus_tag="RHZ76_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HDV2812197.1"
                     /translation="MAGLKATHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIRLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAIMGFISFVGLLLAMPETVKRGAVSFSAKSVLRDFRNVFCNRQFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEEQ"
     gene            18240..19520
                     /locus_tag="RHZ76_001541"
     CDS             18240..19520
                     /locus_tag="RHZ76_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HDV2812198.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKVDINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            19636..20787
                     /locus_tag="RHZ76_001542"
     CDS             19636..20787
                     /locus_tag="RHZ76_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HDV2812199.1"
                     /translation="MSLVTDLPAIFDQFSEARQTGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            20797..21564
                     /locus_tag="RHZ76_001543"
     CDS             20797..21564
                     /locus_tag="RHZ76_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812200.1"
                     /translation="MAYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTNVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQRF
                     THMLESHLGMPVQTHPDAQFAGAIGAAVIGQRQRKRA"
     gene            21561..21818
                     /locus_tag="RHZ76_001544"
     CDS             21561..21818
                     /locus_tag="RHZ76_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDV2812201.1"
                     /translation="MKEYLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGHTESVYCQQDGGWRCIAHYGISPR"
     gene            21883..22743
                     /locus_tag="RHZ76_001545"
     CDS             21883..22743
                     /locus_tag="RHZ76_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HDV2812202.1"
                     /translation="MTKSISLSKRIFVIVILFVIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDLIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNIYIVSEPNR
                     FYRFTPQSSH"
     gene            22811..23989
                     /locus_tag="RHZ76_001546"
     CDS             22811..23989
                     /locus_tag="RHZ76_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2812203.1"
                     /translation="MPSSTHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     PPFILVLLIRVLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMMLIALTLLMPSKKHAITPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(24002..24556)
                     /gene="kptA"
                     /locus_tag="RHZ76_001547"
     CDS             complement(24002..24556)
                     /gene="kptA"
                     /locus_tag="RHZ76_001547"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HDV2812204.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GLPFWQAENGVWLTSTVAVEFLEW"
     gene            24806..25489
                     /locus_tag="RHZ76_001548"
     CDS             24806..25489
                     /locus_tag="RHZ76_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812205.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            25486..25947
                     /locus_tag="RHZ76_001549"
     CDS             25486..25947
                     /locus_tag="RHZ76_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDV2812206.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            25960..27132
                     /gene="iadA"
                     /locus_tag="RHZ76_001550"
     CDS             25960..27132
                     /gene="iadA"
                     /locus_tag="RHZ76_001550"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDV2812207.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            27197..28108
                     /gene="hypT"
                     /locus_tag="RHZ76_001551"
                     /gene_synonym="qseD"
     CDS             27197..28108
                     /gene="hypT"
                     /locus_tag="RHZ76_001551"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HDV2812208.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(28101..28493)
                     /gene="iraD"
                     /locus_tag="RHZ76_001552"
     CDS             complement(28101..28493)
                     /gene="iraD"
                     /locus_tag="RHZ76_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HDV2812209.1"
                     /translation="MMRQSRQAVLPEISGNKTSSLRKSICSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYSHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(28490..28573)
                     /gene="idlP"
                     /locus_tag="RHZ76_001553"
     CDS             complement(28490..28573)
                     /gene="idlP"
                     /locus_tag="RHZ76_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HDV2812210.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         6699 a         7507 c         7289 g         7310 t
ORIGIN      
        1 tcattatagt gataatattc atgtgatagt ttgtttgcgg cgagagataa ttcgcttttt
       61 atcaccgagc ttgtcccgat aaccaggatt aatcagcgca attttgatct taacctaccg
      121 caaaacaacc gtgcttcatg atacaaatgg attctcatta tctgaacaat acgcagcacg
      181 tctatgacaa agggcgagtt atgcaaactg agcaacagcg agccgtaacg cggctttgta
      241 tccagtgtgg attatttctt ttgcaacatg gtgcggaaag cgcgttggtt gatgagcttt
      301 cctcacgact gggtcgggcg ttgggaatgg acagcgtcga aagttctatc tcttcgaacg
      361 ccatagtgct gacaactatt aaagatgggc aatgcctgac atcgacacga aaaaatcacg
      421 atcgcggcat taatatgcat gtggtgactg aagtccagca cattgtgatt cttgcggagc
      481 atcatctgct ggattacaaa ggcgtagaga aacgatttag ccaaattcag ccattacgtt
      541 acccaagatg gctggtagcc ttaatggttg gcctttcttg cgcctgtttc tgtaaactca
      601 ataacggtgg ctgggatggt gccgtcatca ccttctttgc cagtacgacc gcgatgtata
      661 tccgccagct gctggcacaa cgtcatcttc atccacagat caacttttgc cttaccgctt
      721 tcgccgccac caccatttcc ggattgcttt tgcaactccc cactttcagc gatacaccca
      781 ccattgcgat ggccgccagc gttctgctgc tggtgccggg ctttccgttg attaatgccg
      841 tcgccgatat gtttaaaggc cacatcaata ccggactggc acgctgggcg atcgccagtc
      901 tgctgacgct ggctacctgc gtcggcgtag tgatggcact gacgatttgg gggctacgcg
      961 gatgggtgtg atcgagtttc tgtttgcatt ggcgcaggat atgatcctcg ccgccattcc
     1021 agcggtcggc tttgcgatgg tgttcaacgt tcccgtgcgg gcgttacgct ggtgtgcgct
     1081 gcttggcgcg attggccatg gttcacgaat gatcttaatg accagcgggc tgaatattga
     1141 gtggtcaact tttatggctt ctatgctggt cggcaccatt ggtattcaat ggtcgcgctg
     1201 gtatctggcg catccgaaag tgtttaccgt ggcggccgtt atccctatgt tcccgggcat
     1261 atcggcttat accgcaatga tttcggcggt aaaaatcagc cagttaggtt acagcgaacc
     1321 gttgatgatt accctgttaa ccaactttct tacagcttca tcgattgttg gtgcgttatc
     1381 catcggtctt tccattcctg gattatggtt gtaccgcaag cgccctcgcg tataaaattg
     1441 cctcgcttaa gcacacggat gagagacagc ctcctctcct ccgtgtgtta ctataaaagt
     1501 tatctccctt ctcgttcatc gttccatatt tgagaaacag tatgtcttcc agagttttga
     1561 ccccggacgt cgttggtatt gacgccctgg tacacgatca ccaaaccgtt ctggcaaaag
     1621 ctgaaggcgg tgtggttgcc gtatttgcta acaatgcccc ggcgttttat gccgtcacgc
     1681 ctgcacgcct ggctgaactg ctggcgctgg aagaaaagct ggcgcgtccg ggaagcgatg
     1741 tcgctctgga cgatcaactc tatcaggaac cgcaagccgc tcccgttgct gtccccatgg
     1801 ggaaattcgc catgtatccg gactggcaac ccgatgccga ctttatccgc ctggcggcgc
     1861 tatggggcgt ggcgctaaga gagccggtga ccaccgaaga actggcctca ttcattgcct
     1921 actggcaggc ggaaggtaaa gtctttcacc atgtgcagtg gcaacaaaaa ctggcgcgca
     1981 gcctgcaaat cggtcgtgcc agcaacggcg gactgccgaa acgagatgtg aatacggtca
     2041 gcgaacctga cagccaaatt ccaccaggat tcagagggta acgatgaaaa acgttggcga
     2101 cctgatgcaa cgcctgcaaa aaatgatgcc tgcccatatc aaacctgcat tcaaaacggg
     2161 tgaagagcta ctggcctggc agaaagaaca aggggcgatc cgctccgccg ctctcgaacg
     2221 tgaaaatcgg gcgatgaaaa tgcagcgcac ctttaaccgc tccggtattc gtccactgca
     2281 tcagaactgc tcctttgaga actatcgcgt tgagtgtgaa gggcagatga atgcgttaag
     2341 caaagcgcgc cagtatgtcg aagagtttga cggcaacatc gccagcttca tcttttctgg
     2401 taagccagga accggcaaaa accatctggc ggcggcaatc tgcaacgagc tgctgctacg
     2461 cggtaaatcc gtattgatca tcaccgtggc cgatattatg tcggcgatga aagatacctt
     2521 caggaatagc ggtaccagcg aagaacaact gcttaacgat ctgagcaacg ttgatctgct
     2581 ggtgatcgat gagatcggcg tgcagaccga atcgaaatac gaaaaagtga tcatcaacca
     2641 gatcgtcgat cgccgctctt cttccaaacg cccaaccggg atgctgacca acagtaatat
     2701 ggaagagatg accaagttgc tgggcgaacg cgtgatggac cgtatgcgcc tgggtaacag
     2761 tttgtgggtg atcttcaact gggatagcta ccgtagccgg gtaacaggta aagagtatta
     2821 atccccgttt ctggcggtat actgcgacac aattccacct tgagtaatga tgatgaaaac
     2881 tgttaaacat cttctgtgct gtgccattgc cgccagcgca ttaatttcca ccggggtgca
     2941 tgctgcgtcc tggaaagatg cgctctccag cgccgccagc gaacttggca accaaaacag
     3001 cacgacacag gaaggcggtt ggtcgctcgc gtcattaact aacttgctta gcagcgggaa
     3061 ccaggcctta agcgcagata acatgaacaa cgccgcaggc attctgcaat actgcgcgaa
     3121 gcaaaagctg gcgtcggtaa ccgatgccga aaacatcaag aaccaggtgc tggaaaagct
     3181 gggcctgaac agtgaagaac aaaaagaaga caccaactat ctggatggca ttcagggttt
     3241 gctgaaaacc aaagatggtc agcaactcaa tctggataac atcggaacga ctccgctggc
     3301 agaaaaggtg aaaaccaaag cctgcgatct ggtgttaaag caggggctga acttcatttc
     3361 ctgaaccgcg aaacgccgcg ttttagctgt tacccggctg acgcggctct ctcctgccct
     3421 tttccctgct cctggacggt tttccccctg ttacacggga agcgaccgcg atcaaaaaag
     3481 tctgtttata aactaattcc taacaacatc gcattttttt gacactagaa ttgcagcaat
     3541 atggcgacgc cattacattg tgcttctcaa aaagcttaaa cgttggctaa acggcccggt
     3601 ttagtgagga tcgcccgttg tcagaactac tctctttcgc cctttttctc gcctctgtgc
     3661 tgatttacgc atggaaagcg ggacgtaaca cctggtggtt tgcagccaca ttaacggtgc
     3721 tggggctatt tgtcgtttta aatatcaccc tgtttgccag cgactatttt actggcgctg
     3781 gtattaacga cgcggttctc tataccttaa ccaacagcct gaccggtgct ggcgtcagca
     3841 aatacatcct gccgggtatc ggcattgtgc tggggctgac agcggtgttc ggtgcgctgg
     3901 gctggatcct gcgccgtcgt cgccatcatc cgcaccattt tggttacagc ctgctggcgc
     3961 tcttactggc gctgggttcg gtggacgcca gcccggcatt tcgtcagata acggaactgg
     4021 tgaaatccca gtcacgcgat ggcgacccgg actttgcggc ttattataaa gagccgtcga
     4081 aaactatccc tgacccgaaa ctcaacctgg tttatatcta cggcgaaagt ctcgagcgga
     4141 cctattttga taacgaagct ttcccggatc tcacgcctga actgggcgcg ttgaaaaatg
     4201 aaggcctgga tttcagccac acgcagcagc tgccaggaac ggattacacg attgcgggca
     4261 tggtggcttc tcagtgcggc ataccgctgt ttgccccctt tgaaggcaac gcctccgcct
     4321 ctgtctccag cttcttcccg cagaacatct gtctgggcga tatcctgaaa aactcgggtt
     4381 atcagaacta tttcgtgcag ggcgcgaatc tgcgttttgc cggtaaagat gtgttcctga
     4441 agtcgcatgg cttcgaccac ttatacggct cagaagagct gaaaagcgtg gtggccgacc
     4501 cgcactatcg caacgactgg ggattctacg acgataccgt tctcgatgaa gcgtggaaaa
     4561 agtttgaaga gctttcccgc tcaggtcagc gattctcact gtttaccctg acagtcgata
     4621 cccatcaccc ggatggtttt atctcgcgta cctgtaaccg caaaaaatat gattttgacg
     4681 gtaagccgaa ccagtcattc agcgcggtaa gttgcagcca ggagaatatc gcggcgttta
     4741 tcaacaaaat caaagcgtca ccgtggttta aagataccgt catcgtcgtc tcttctgacc
     4801 atttagcgat gaacaacacg gcgtggaaat acctcaataa gcaggatcgc aataacctgt
     4861 tttttgtcat tcgtggcgac aagccgcagc aagagacgct ggcagtgaag cgtaacacga
     4921 tggataacgg cgcgacggtg ctggacattc tcggtggcga taactatctc ggacttggtc
     4981 gtagcagttt atccgggcag tcaatgtcgg aaatcttcct caatatcaaa gagaaaacat
     5041 tggcgtggaa gccggatatc atccgcctgt ggaaattccc taaagagatg aaagagttca
     5101 ccatcgacca gcagaaaaac atgattgcct tctcgggtag ccatttccgt ttgccgctgc
     5161 tgttgcgggt ttcagacaaa cgcgtggaac cgctgccgga aagcgaatac tcagcaccgc
     5221 tgcgtttcca gctggccgat ttcgctccac gcgacaattt cgtctgggtt gaccgttgct
     5281 ataagatggc acaactctgg gctccggaac tggcactctc caccgactgg tgtgtctcgc
     5341 aagggcagct tggcggtcag caaattgttc agcatgttga caaaacaaca tggcagggca
     5401 aaacggcatt taaagatacg gtcatcgaca tggcgcgtta taaaggcaat gtcgatacgc
     5461 tgaaaattgt tgataacgat attcgctaca aagccgacag tttcatcttc aacgtcgccg
     5521 gtgcgccaga agaggtgaaa cagtttagcg ggatttcccg tccagagtcg tggggccgct
     5581 ggtccaacgc gcagctgggc gatgaagtaa aaatcgagta caagcatccg ctgccgaaga
     5641 aatttgacct ggtgattacc gccaaagcat acggcaataa cgccagccgt cctattccgg
     5701 tacgcgtagg caatgaagaa caaactcttg tgctgggcaa tgaagtgacc accaccacac
     5761 tgcatttcga taacccaacc gatgccgaca cactggtaat tgtgccgccg gaacctgtct
     5821 caaccaacga agggaatatc ctcggacact cgccgcgtaa gctcgggatc ggcatggtgg
     5881 aaattaaagt tgtggaacgt gaagggtaat ttctgactga tgtgtaggtc gcataaggtg
     5941 cgacctacac atcttctccg gcctacaaaa catcattgag aaacagaaaa aaaaacagcg
     6001 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6061 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6121 tggcgaacgg atagcgatgg gttaacatca tgtcagcagt gatttttcct tccgccatca
     6181 gtcgaccgac tttagcaaaa tcttccggcg tggcgttgcg gctacccatc atcgtcgttt
     6241 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacagg ccgacaaata
     6301 ccaccgtgcc gccgtgacga atcaaattca cggtgttatt catcgcatgt tgattacctg
     6361 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa
     6421 aatcttcggc tgacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6481 gcgctggact ggtatccgcc accaccacct gtgcgccatc agctttagcg attgccgccg
     6541 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6601 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     6661 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     6721 catcctgatg cacgccaatg actgaaattt tttcacagca attggtacgc ccgcttttac
     6781 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     6841 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaacac
     6901 gtggataact aaaaaatggt tgatttccac cccaggcatg aatatcggta ccgcaaatcc
     6961 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7021 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7081 tctccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7141 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7201 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7261 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7321 gtacaacatc agccgcacta cagtgcgtca tattctcagc cacttacgcg aatgcggcgt
     7381 cttaacgcag gttggaaacg actatgtgat tgcgcgtaaa cctgaccatg atgacggttt
     7441 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7501 gatgataaac cagcgccagt tacgccctgg ggaaactttt tctgaactgc aactggctcg
     7561 ggccgcgggt gtcagcccgg ttgtagtaag agaatatctt ttaaaattcg gccgttataa
     7621 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     7681 cgagcagctg tttgaactac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     7741 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctcgaac gtcatcggct
     7801 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     7861 ctccttactg ctttcagctg ccgacaatat cttttttgat caatctcttg agatcatctc
     7921 agtcatcttt cattttcact atcaatggga cgaaagcgat cttaaacagc gcaatatcat
     7981 tgccgtcgat gagcatatga ccattctcag cgctctgatt tgccgcagtg accttgatgc
     8041 cacccttgcg ctccgtaacc atttgaattc agctaaacaa tcaatgattc gatccattaa
     8101 tgagaacatg cgttatgctc attaagtacg gattaaaaac agcaaactat tagcgggtgc
     8161 acggttttat caaaagataa ataccggaaa attcccgctt acctatgctc acaatcagtc
     8221 gactaaaaat gtttttttgt gagcggtagt aaagtcctaa aactttaacc tgccgttaat
     8281 gtcaaccttg cgtaatgcac aattacgtaa acaattaaaa ttataaatac aggagtccgg
     8341 cgtggaaaaa gaaaatatca ccctcgatcc gcgttcttca tttactccat cttcgtcggc
     8401 agatattccc gtgccaccag atggattagt tcaacgcagt acccgaatta aacgcattca
     8461 aaccaccgcc atgttgttat tattttttgc ggcggtaatc aattatctcg accgcagttc
     8521 gctgtcggta gcaaatttaa cgattcgtga agaattggga ttaagtgcca ccgaaatcgg
     8581 cgctttgctc tccgtgtttt cactcgctta cgggattgcg caacttcctt gcggcccact
     8641 attggatcgt aaaggcccac gcctgatgct gggactgggg atgttcttct ggtcactgtt
     8701 ccaggccatg tctggcatgg tgcacagctt tacgcagttc gtgttggtgc gtatcggtat
     8761 ggggattggt gaagcgccga tgaacccatg tggcgtaaaa gtcattaacg actggttcaa
     8821 catcaaagag cgcggacgcc cgatgggctt cttcaacgca gcttctacca ttggcgttgc
     8881 cgtaagccca ccgattctgg cggcgatgat gctggtgatg ggctggcgtg ggatgtttat
     8941 taccattggt gtgctgggga tttttctcgc cattggctgg tatatgctct atcgcaaccg
     9001 cgagcacgta gaactgactg ccgttgaaca agcttatctc aatgcaggta gcgtcaatgc
     9061 ccgccgagat ccgctcagtt ttgccgaatg gcgcagcctg ttccgtaacc gcacaatgtg
     9121 gggaatgatg ctcggattca gtggcatcaa ctacactgcg tggctgtatc tggcctggct
     9181 tcctggttac ctgcaaacag cctataacct ggatttaaaa agcacagggt tgatggcggc
     9241 tatccctttc ctgtttgggg ctgccgggat gctggtcaac ggttacgtta ctgactggct
     9301 ggtcaaaggg ggaatggctc cgattaaaag ccgtaagatc tgcattattg ccgggatgtt
     9361 ctgttctgcc gcctttacgc tggtcgtacc gcaagcgaca acatccatga cggcggttct
     9421 gctgattggc atggcactgt tctgtattca ctttgccgga acatcctgct ggggcttgat
     9481 ccacgtcgca gttgcttctc gcatgacggc gtcggtgggc agtatccaga actttgccag
     9541 cttcatctgc gcctcttttg cgccgatcat tactggtttt attgttgata ccacccactc
     9601 attccgtctg gcactaatca tctgcggttg cgtcaccgca gcgggagcac tggcgtacat
     9661 cttcctggtt cgtcagccga tcaacgaccc gcgtaaagat taaactccct atgcccgata
     9721 acattttgct tatcgggcat ttttatggcg attaaaacgt ttcccagttc tccccgctat
     9781 ctgccactgc cattttacgc ggcgtagctg gcgtcacgtt ttttaccaca gccgatgttt
     9841 cacgctgctg ttgctgctgt tgctgaatcc ggaacactgc cacagcttcg gtcaggcgac
     9901 tggcctgctc ttccagcgcg gcggcggcag cggcagattc ttccaccagc gcagcgttct
     9961 gttgagttac ccggtccatc tcagcaaccg ctaagccaac ctgatcgata ccacggctct
    10021 gctcatcaga agcagaagca atttcgccca taatgtccgt cacgcgggtc acggcactga
    10081 caatctccgc cattgtttcc cctgcgcttt cgaccagcgt agagccaaca tctactttcc
    10141 ccaccgagtc ttcaatcagg cttttaattt cacgagccgc ctgagcgctg cgctgagcca
    10201 gattacgcac ttcacccgcg accaccgcaa aaccacgtcc ttgctcaccc gcacgcgctg
    10261 cttcaaccgc cgcgttcaaa gccagaatat tggtctggaa ggcaatgccg tcaattacgc
    10321 tgataatatc ggcgattttc tgcgaactgg tggagatatc acgcatagtc tgcaccacgt
    10381 tatccaccac tttgccgccg cgttgcgccg tttcagaagc acttaacgcc aggtggctgg
    10441 cctggcgcgc attctcggcg ttctgtttca ccgttgcggt cagttgctcc atgctggctg
    10501 ccgtctcttc cagcgaagca gcctgttgct cggtgcgcga agagagatcg ttattgccgg
    10561 tagcgatttc gctggcaccg ctatagatgg cattggcccc gttgcgcaca tcaccgacgg
    10621 tacgcatcag ctctccctgc atatggcgca aactctctgc cagttgcccc atctcattag
    10681 agccatccac ctcaatcggt ttcaccagat cgccgcctgc aatatgacga atgctgtcaa
    10741 tcaggcgatt cattggcgct accagcgagg ctttaatacc gaaccagacg gcgaagatga
    10801 ccgccagtac gacgatcatc acgcccacca gaatccacat cgcctggctg taggaggcat
    10861 tgttatcgct gacggcgata tcatggagcc gatcgttttg ttccatgtaa gccacatact
    10921 gcttctcgaa accgtcctga tatccctgag tcggctgatc aaagaactcg ttgattttgc
    10981 ctgcacctaa cagttggatc agctccgcca gcgcattgtg ataaatatcg taattacgtt
    11041 tgatctctgc cgctgcggcg gtgctctgac gcgggtcacg cggcaacgct tcgtaatccg
    11101 cccagttttt ttccgcctgt ttcagcgaaa tactggcact ctgcatcagc tcagcaacgg
    11161 ttgaaccgct accaatatta ttctgatcca tcatgtagcg gatacccgcg cggttgaggg
    11221 tgttacgcgt ctgcaacaac gcgacccagc tgccattcag cgtggattgc tgctggcgaa
    11281 tggtttgtaa aacagtgaaa ttttctttgt cattctttaa ggcattaaag aacagaccgc
    11341 ctgatgtcag ttgtaaaagg ccaaaaacgg ccaaaaccag cagtaagctg gtcacaattt
    11401 tgatacgttt taacatggtt tctctttcct gtggaccgat gcaagatttt cggcctggaa
    11461 aggaaaaact ttatgaatat cccgcggaga ttacgccgag taattttatt cacactctga
    11521 atttaaaaag ccattattta cataaattat tcacaatata aatatgtgat atgaatcaca
    11581 tatttatcgt cacttaaacg acgcctttgc cgctcaaccg caaaactgac cgcttacatc
    11641 cctaaaataa ccactcagtt atttacctta ctttacgcgc gcgtaactct ggcaacatca
    11701 ctacaggata gcggtcaatt tacctcctca aacgcaacgc aaacctagaa cggcttcggc
    11761 caactattaa tcaatacatg ccaggtttta ctatggatac taaaaagata ttcaagcaca
    11821 taccctgggt gattctcgga atcatcggtg cattctgcct cgcggtagtt gcattacgtc
    11881 ggggggagca cgtcagcgcc ctgtggatcg tggtcgcctc tgtgtcggtg tatctggtgg
    11941 cttatcgcta ctacagtctg tacatcgccc agaaggtgat gaaactcgac cccacgcgcg
    12001 cgacgcctgc ggttattaac aacgacggtc tgaactatgt tccgaccaac cgttacgtgt
    12061 tgtttggtca ccacttcgcc gctatcgccg gtgctggtcc gctggttggt ccggttctcg
    12121 ccgcgcagat gggctacctg cccggcacgc tgtggctgct ggcgggggta gtgctggccg
    12181 gtgcggtaca ggactttatg gtgctgttta tctcctctcg ccgtaacggc gcatctctcg
    12241 gtgagatgat caaagaagag atggggccag taccgggaac tatcgcgctg tttggctgtt
    12301 tcttaatcat gatcatcatc ctcgccgtcc tggcgctgat tgtggttaaa gccctggccg
    12361 aaagtccgtg gggtgtcttc accgtttgct caaccgtacc gattgcgctg tttatgggta
    12421 tctacatgcg ctttatccgt ccggggcgtg tgggtgaagt ctctgttatt ggtatcgtgc
    12481 tgctggttgc ctctatctac ttcggtggcg tgattgctca cgacccgtac tggggtccgg
    12541 cactgacctt taaagacacc accattacct tcgcgctgat tggctatgcg tttatttccg
    12601 cgctgctgcc ggtgtggctg atcctcgcac cacgcgacta tctggcaacc ttcctgaaaa
    12661 tcggcgttat cgtcggcctg gcgctgggta tcgtggtgct gaacccggaa ctgaaaatgc
    12721 ctgcgatgac ccagtacatt gacggtactg gcccgctgtg gaaaggcgct ctgttcccgt
    12781 tcctgttcat caccatcgcc tgtggtgcgg tatctggctt ccacgcgctg atctcttccg
    12841 gtacgacgcc gaaactgctg gctaacgaaa ccgacgcgcg tttcattggc tacggcgcaa
    12901 tgctgatgga gtccttcgtg gcgattatgg cgctggttgc tgcgtccatc atcgaaccgg
    12961 gtctttactt cgcgatgaat accccgcctg ctggccttgg catcaccatg cctaacctgc
    13021 atgaaatggg tggcgagaac gcgccgatca tcatggcgca gttgaaagac gttaccgcac
    13081 acgcggcagc gaccgtcagc tcctggggct tcgtgatttc gccagagcag atcctgcaaa
    13141 ccgcgaaaga cattggtgag ccttctgtcc tgaaccgtgc aggtggtgcg ccgacactgg
    13201 cggtaggtat cgctcacgtg ttccacaaag tgctgccgat ggctgacatg ggcttctggt
    13261 atcacttcgg tattctgttc gaagccctgt tcatcctgac cgcgctggat gcgggtactc
    13321 gttctggccg ctttatgctg caagacctgc tgggtaactt catcccgttc ctgaaaaaaa
    13381 ccgactctct ggttgccggt atcatcggta ctgcgggctg tgtgggtctg tggggctacc
    13441 tgctgtatca gggcgtggtc gatccgctgg gcggcgttaa gagcctgtgg ccgctgttcg
    13501 gtatttccaa ccagatgctg gcagccgtag cgctggtact gggcaccgtt gtgctgatta
    13561 agatgaagcg cacccaatac atctgggtaa ctgttgttcc ggctgtatgg ctgcttatct
    13621 gcaccacctg ggcgctgggt ctgaaactgt tcagcaccaa cccgcagatg gaaggcttct
    13681 tctacatggc aagccagtac aaagagaaga ttgctaacgg tactgacctg acggcgcagc
    13741 agattgccaa catgaaccac atcgttgtga acaactacac caacgcaggc ctgagtattc
    13801 tgttcctgat tgtggtgtac agcatcatct tctacggttt caaaacctgg cttgcggtgc
    13861 gtaacagcga caaacgtact gacaaagaaa ctccgtacgt tccaatcccg gaaggcggcg
    13921 tgaagatttc ttcgcaccac taaccgtgtt tagccccgct tcggcggggc tttgttctta
    13981 tcagagtgaa ctatgtttgg taacttagga caggcaaaaa aatatctcgg ccaggcggcg
    14041 aagatgttga ttggcattcc agactacgac aactacgtcg agcatatgaa gaccaaccat
    14101 cccgacaagc cgtacatgag ctatgaagaa ttcttccgcg aacgccagaa cgcgcgctac
    14161 ggcggcgatg gtaaaggcgg tatgcgctgt tgttaatgga gagagcatga acccgattgc
    14221 agttacccta ctcaccggtt ttttaggcgc gggcaaaacc accctgctgc gccatattct
    14281 taacgaacaa cacggctaca agattgccgt gattgaaaac gaattcggcg aagtctccgt
    14341 tgatgatcaa ctgattggcg accgcgccac gcagatcaaa acgctgacca acggctgcat
    14401 ctgctgttcg cgctccaacg agctggaaga cgcactactc gacctgctgg ataatctcga
    14461 caagggcaat attcagttcg accgtctggt cattgaatgc accggcatgg ccgatcccgg
    14521 cccgattatt cagacctttt tctcccatga gattttatgc cagcgctacc tgctggacgg
    14581 ggtgattgcg ctggtggatg cggtacatgc cgatgagcag atgaaccagt tcaccatcgc
    14641 ccagtcacag gttggctacg ccgaccgtat tctgctgact aaaaccgacg tcgcaggcga
    14701 agcagagaaa ctgcgtgaac gcctggcgcg catcaacgcc cgtgcaccgg tctacaccgt
    14761 cacccacggc gacatcgacc tgggccttct gttcaacacc aacggtttta tgctggaaga
    14821 aaacgtcgtc agcaccaaac cgcgtttcca ctttatcgcg gacaaacaaa acgatatttc
    14881 gtcgattgtg gtggaactgg attacccggt agatatcagc gaagtttccc gggtgatgga
    14941 aaacctgctg ctggagtcgg cggataaact actgcgttac aaagggatgc tatggattga
    15001 cggcgaacct aaccgcctgc tattccaggg cgtccaacgc ctctacagcg ccgactggga
    15061 ccgcccgtgg ggcgatgaaa aaccgcatag cacgatggtg tttatcggga ttcagttgcc
    15121 ggaggaagag attcgggcag cgtttgcggg gttgaggaag taattttgga tgatggccgc
    15181 catgtggcgg cctgaattat tttttgttct gatcgcgcag catcgcttca cgtaaaattg
    15241 cgttcaggcg agtttgatag cctttacctg gtcgcttcaa ccattccatc acatcagcat
    15301 caatacgcac cgaagcctga gttttcaatg gccgataaaa cttaccgcgt tcggcgttag
    15361 accattgctc atcgctggaa ggtgggatat cactgtaatc aatatcttcg tcggatttat
    15421 tcgccagcgc cttcagttcg gcctcatgct gcacatcgag tgtagaggag gtccctcgtt
    15481 tatgtttaac catgctcata gcgattcctc tctttacgat ctgcttttcg tgcactgatg
    15541 attcgaataa tttcatttcc gctttcgaaa cggatggtat gcgcaaccag aataaccacg
    15601 atgccgtaaa ccaggccgat tgtctgccag cgatactcac cgttttctat gcgttcttgc
    15661 tgtgacaaat gttgtggatc atcaaacaat aacaccgcat cttcaaagcg gatgccatgt
    15721 ttcacccgat tacttttggc cttgttttca tcccattcaa actccattgg cataattgcc
    15781 atccctaatt gtatatacaa aaatgtatct acaattattc tgcaattata gcgttgatgt
    15841 tggaaatagc atcacgtttc gaataatcaa ccatgaaatt gacaatgagt aagttggact
    15901 tgattcatcc aatagaattt ctcgcctgac atggcacact aaatacctcg ctgtcgggta
    15961 atgactccaa cttactgata gtgttttatg ttcagataat gcccgatgac cttgtcatgc
    16021 agctccaccg attttgagaa cgacagtgac ttccgtccca gccttgccag atgttgtctc
    16081 agattcagat tatgtcgctc aatgcgctga gtgtaacgct tgctgataac gtgcagcttt
    16141 cccttcaggc gtgattcata cagcggccag ccatccgtca tctataccac gacctcaaag
    16201 gccgacagca ggctcagaag acgttccagt gtggccagag tgcgttcacc gaagacgtgc
    16261 gccacaaccg tcctccgtat cctgtcatac gcgtaaaaca gccagcgctg acgtgattta
    16321 gcaccgacgt agccccactg ttcgtccatt tcagcgcaga caatcacatc actgcccggt
    16381 tgtatgcgcg aggttaccga ctgcggcctg agttttttaa gtgacgtaaa accgtgttga
    16441 ggccaacgcc cataatgcgt gcactggcgc gacatccgac gccattcatg gccatatcaa
    16501 tgattttctg gtgcgtaccg ggctgagagg cggtgtaagt gaactgtagt tgccatgttt
    16561 tacggcaatg agagcagaga tagcgctgat gtccggcagt gcttttgccg ttacgcacca
    16621 cgccttcagt agcggagcag gaaggacatc tgatggaaat ggaagccacg caagcacctt
    16681 aaaatcacca tcatacacta aatcagtaag ttggcagcat tacccggatt ccataatgat
    16741 tccacgcccc gttatgacaa atctgccgcc gatcttgcct ggcaaaggac actgaaatgg
    16801 tttgataaat atctctcctg ataggtttat ctcttacggg attacgtctt aaacaagcat
    16861 gaaaaaatag cgtgcgcaaa agtcgttctt tgcctaaaac atcgctatat atggcgatat
    16921 atagcgaata aggtgaacga tgaataacca ttttggtaaa ggcttaatgg cgggattaaa
    16981 agcaacgcat gccgccacgc tgtttttccc gatggcgttg attttgtatg attttgccgc
    17041 gtacctgtcg acggacctga ttcagccggg gatcattaat gtggtccgtg attttaatgc
    17101 cgatgtcagt ctggccccgg cagccgtcag tctctatctc gcgggcggta tggcgttaca
    17161 gtggctgctg gggccgcttt ccgacaggat tggccgcagg ccggtgctga ttaccggggc
    17221 gctaatattt acccttgcct gcgctgcgac aatgttcaca acgtctatga cacagtttct
    17281 tatcgcgcgt gcaattcagg gcaccagtat ctgttttatt gccaccgttg gttatgtcac
    17341 ggtgcaggag gcgttcggcc agacaaaagg gatcaggttg atggcgatta tcacctccat
    17401 cgtactgatt gcgccgatta tcggcccgct ttccggcgca gctctgatgc actttgtaca
    17461 ctggaaagtc ctttttgcca tcattgcgat tatgggtttt atctcatttg ttggcttact
    17521 gctggcgatg ccagagacgg tgaagcgcgg cgcggtttca tttagcgcca aaagcgtctt
    17581 gcgcgatttt cgtaatgtct tttgcaatcg gcagttcctc tttggtgcag caaccatctc
    17641 tttaagctat atcccaatga tgagttgggt ggctgtctcg ccggtgatcc tgattgacgc
    17701 aggcggctta acgacctcgc agttcgcctg gacacaggtt ccggtgttcg gcgcggtgat
    17761 tgtcgcgaat gccatcgtgg cgcgtttcgt taaagatccg accgaaccgc gctttatctg
    17821 gcgtgccgta cccattcaac tggttggcct cgcgctgttg attatcggca atctgctgtc
    17881 accgcacgta tggctgtggt cggtgctggg caccagtttg tatgctttcg ggattggttt
    17941 gatcttcccg accttattcc gctttacgct gttttccaat aacttaccga aagggaccgt
    18001 ctccgcatcg ctgaatatgg tgatcctgat ggtgatgtct gtctcggtcg aaatcggccg
    18061 gtggctgtgg tttaacggcg gtcgcttgcc gtttcatctg ttagccgtcg tggcgggcgt
    18121 tatcgtcgtt ttcaccctgg cgggattgct taatcgcgtg cgccaacatc aggctgccga
    18181 gctggcagag gagcagtgat ttttgcgcga tcctgccgtc aggctctatt cttaacgtta
    18241 tgaataaact cattgaactc agacgcgcca aaatgttggc gctctcttta ctgcttatcg
    18301 ccgctgctac ctttgtcgtt acgctgtttt tgccgcccaa tttttgggtg agcggcgtga
    18361 aggcgattgc tgaagcggcg atggtcggcg cgctggcgga ctggtttgcg gtggtggcgc
    18421 tgtttcgccg cgtgccgatt ccgatcattt ctcgccatac ggcgattatc ccgcgtaata
    18481 aagaccggat tggcgaaaat ctcggccagt tcgtgcagga aaaatttctt gatactcaat
    18541 ccctggtggc attgattcga cgccacgaac cggcgttgct gattggcaac tggtttagcc
    18601 agccagaaaa cgcccgccgc gttggtcagc atctgttgca gatcatgagc ggttttctcg
    18661 aacttaccga tgatgcgcgt attcagcgcc tgcttaagcg cgcggtccat cgggcgattg
    18721 ataaggtcga tctttccggc accagtgcgt tgatgctgga gagtatgacc aaaaacgatc
    18781 gtcatcaggt gctgctggat acgctgatcg cacagttgat cgcccttctc cagcgcgata
    18841 aatcgcgcaa gtttatcgcc cagcaaattg ttcgctggct ggagagcgag catccactga
    18901 aagccaaaat tctccccacc gaatggttgg gcgaacatag cgcggagttg gtttctgacg
    18961 cggtgaattc tttgcttgat gatattagtc gcgatcgtgc gcatcagatc cgccatgcgt
    19021 ttgatcgcgc caccttcgcc ctgatcgaca agctgaaaaa cgatccggaa atggcagcgc
    19081 gagccgatgc cgtaaaaagc tatctgaaag aagatgaagc ttttaatcgc tatctcagtg
    19141 aattgtgggg ggatttacgg gaatggctga aagtggatat caacagtgaa gattctcgtg
    19201 tgaaagaacg catcgcacga gcgggtcaat ggtttggcga aacgttaatt gccgatgatg
    19261 ccttgcgggc gtcgttaaat ggtcatcttg aacaagccgc gcaccgcgtc gcgcctgagt
    19321 tttccgcatt cctgacgcgc cacatcagcg atacggtaaa aagctgggat gcgcgggata
    19381 tgtcgcggca aatagagtta aatatcggca aagatctgca gtttattcgt gtcaacggta
    19441 cgctggttgg cggttgtatt gggctaattt tatatttgct gtcgcagctc ccggccttgt
    19501 tccccctcgg caatttttag aaatccatga aaaactaagt ggctaaccgc gagagagatc
    19561 aaataaacca tactaaaaga atggtaataa tgtggcctct tttcattatc tcccgtggta
    19621 cggggaagga aaatcatgtc acttgtcacc gatctacccg ccattttcga tcagttctct
    19681 gaagctcgcc agacaggctt tctcaccgtc atggatctca aggagcgcgg cattccgctg
    19741 gttggcactt actgcacctt tatgccgcaa gagatcccga tggcagccgg tgcggttgtg
    19801 gtttcgctct gttccacctc tgatgaaacc attgaagaag cggagaaaga tctgccgcgc
    19861 aacctctgcc cgctgattaa aagcagctac ggcttcggca aaaccgataa atgcccctac
    19921 ttctactttt cggatctggt ggtcggtgaa accacctgcg acggcaaaaa gaaaatgtat
    19981 gaatacatgg cggagtttaa gcctgttcat gtgatgcaat tgcccaacag cgttaaggac
    20041 gatgcctcgc gtgcgttatg gaaagccgag atgctgcgct tgcaaaaaac ggtagaagaa
    20101 cgttttgggc acgagattag cgaagatgct ctgcgcgatg ccattgcgct gaaaaaccgc
    20161 gaacgtcgcg cactggctaa tttttatcat cttgggcagt taaatcctcc ggcgcttagc
    20221 ggcagcgaca ttctgaaagt ggtttacggc gcaaccttcc ggttcgataa agaggcgttg
    20281 atcaatgaac tggatgcaat gaccgcccgc gttcgtcagc agtgggaaga aggccagcga
    20341 ctggacccgc gtccgcgcat tttaatcacc ggctgcccga ttggcggcgc agcagaaaaa
    20401 gtggtgcgcg cgattgaaga gaatggcggc tgggttgtcg gttatgaaaa ctgcaccggg
    20461 gcgaaagcga ccgagcaatg cgtggcagaa acgggcgatg tctacgacgc gctggcggat
    20521 aaatatctgg cgattggctg ctcctgtgtt tcgccgaacg atcagcgcct gaaaatgctc
    20581 agccagatgg tggaggaata tcaggtcgat ggcgtagttg atgtgatttt gcaggcgtgc
    20641 catacctacg cggtggaatc gctggcgatt aaacgtcatg tgcgccagca gcacaacatt
    20701 ccttatatcg ctattgaaac agactactcc acctcggatg tcgggcagct cagtacccgt
    20761 gtcgcggcct ttattgagat gctgtaagga gtggcagtgg catattcgat tggcattgat
    20821 tccggctcaa ccgccaccaa agggatctta ctggcagacg gcgtgattac gcgccgtttc
    20881 ctcgttccaa caccctttcg cccggcaaca gcaattactg aagcctggga aactctgcgc
    20941 gaagggttag aaacaacgcc gtttctgacg ctcaccggct acggacggca actggtggat
    21001 tttgccgata aacaggtgac ggaaatctcc tgtcacgggc tgggcgcacg gtttcttgcg
    21061 ccagcaacgc gcgcagtaat cgacatcggt ggtcaggaca gcaaagtgat tcagcttgat
    21121 gatgacggta acctgtgcga tttcctgatg aatgacaaat gcgcggcggg caccgggcgt
    21181 ttcctggagg tgatctcgcg cacgcttggc accaacgtcg aacagctcga cagcattacc
    21241 gaaaatgtca cgccgcacgc catcacgagt atgtgcacag tatttgccga atcagaagtg
    21301 atcagcctgc gctcagcggg cgtcgcgcca gaagcgattc tcgccggagt gattaacgcg
    21361 atggcgcggc ggagtgccaa tttcattgct cgtctctcct gtgaagcgcc gattctgttt
    21421 actggtggcg ttagccactg tcaaaggttt acccacatgc tggaaagcca tctgggaatg
    21481 ccggtacaaa ctcaccctga tgcgcaattc gctggtgcaa ttggcgcggc ggtgatcggt
    21541 cagcggcaga gaaaacgcgc atgaaagagt atctattttt atttcactcc acggtcggcg
    21601 tcatacaaac ccgcaaggcg ttgcaggcag cgggcatgac ttttcgcgtc agcgatattc
    21661 cccgtgattt acgcggcggc tgcgggttat gcatttggct gacctgtccg cctggcgagg
    21721 aaattcaatg ggtgatccct gggcatacgg aatcagttta ttgtcagcag gatggtggct
    21781 ggcgctgcat tgcacattac gggatttctc cgcgttaagg attcattaag atcgctacgt
    21841 atacttctcg atatcttatt gatttgcctt aaagggatta ccgtgacaaa aagtatttcg
    21901 ttaagtaaac gcatcttcgt tattgtgatt ctttttgtca ttgtggcagt gtgtactttc
    21961 ttcgtgcaat cttgcgcccg taaaagcaat catgcagcat catttcagaa ttaccacgcc
    22021 accattgacg gcaaagaaat tgcaggcata acgaataata tctcttcgct gacctggtcg
    22081 gcacaaagca atactctttt tagcactatc aacaaacctg ccgccattgt tgaaatgacc
    22141 accaatggtg atttgatccg cacaattcct ctggattttg ttaaggatct cgagacgatc
    22201 gaatacattg gcgataatca gtttgtcatt agcgatgagc gcgattatgc catttatgtg
    22261 atttcactga ccccaaactc ggaagtaaaa atcctcaaaa aaataaaaat cccgctgcag
    22321 gaatccccaa ccaattgcgg ttttgaagga ttggcctatt ctcgccagga tcacactttc
    22381 tggtttttta aagagaaaaa cccgattgag gtctacaaag ttaacgggct attaagtagc
    22441 aatgaattgc acatcagcaa agacaaggca ctacaacggc aatttacttt agatgatgtg
    22501 tccggcgcag aatttaacca acaaaaaaat acgcttctgg tgctgtctca tgaatctcgc
    22561 gcactccagg aagtaacgct ggttggcgag gttatcggtg aaatgtcact aaccaaaggc
    22621 agtcgaggtt tgtcgcataa catcaaacag gccgaagggg ttgcgatgga tgcctctggt
    22681 aatatttata tcgttagcga accaaatcgt ttttatcgct ttacccctca atcatcgcat
    22741 taacaggctg gcatgcttca cctttttgtt attaacttgt taacaacaaa aatgacaaac
    22801 aaggttaaac atgccttcgt ccacgcatcc cgtagaacgc ttttctttca gcaccgcgct
    22861 tttcgggatg ctggttctga ccttaggtat gggtttaggc cgcttccttt atacgcctat
    22921 gttgcccgtc atgatggcgg aaggatcgtt ttcatttagc cagctctcgt ggattgccag
    22981 cggcaactat gcggggtatc tggctggcag tctgctattt tcttttggcg catttcacca
    23041 gccatcgcgc ctgcgcccat ttctgttggc ttctgccctg gcgagcgggt tgttgatcct
    23101 cgcaatggca tggttgccgc catttattct ggtgttattg attcgcgtcc tggcgggtgt
    23161 cgccagcgcc ggtatgctga tttttggttc gacgctgatt atgcagcaca cgcgccatcc
    23221 ttttgtgctg gcagctttgt tttctggcgt tggcattggc atcgcactgg gcaatgaata
    23281 tgttctggca ggcctgcatt ttgacctctc ctcgcaaacg ttatggcaag gcgccggggc
    23341 gctttctggc atgatgctga ttgcacttac gcttttaatg ccctcgaaaa aacacgccat
    23401 cacaccaatg ccattggcaa aaacggagca acagataatg agctggtggt tactggctat
    23461 tctgtacggc ctggcgggat ttggttatat catcgtcgcg acttacctgc cgctcatggc
    23521 gaaagatgca ggctcaccat tgttaaccgc ccatctctgg acgttagtcg gcttatcaat
    23581 tgtgcctggt tgctttggct ggctatgggc agcaaaacgt tggggggctt tgccctgcct
    23641 gacggcgaat ttgctggtgc aggctatctg tgtgctgctt actctcgcca gcgactcgcc
    23701 tctcttgctt atcatcagca gtcttggttt tggcggtacc tttatgggca cgacatctct
    23761 ggtgatgact atcgcccgcc aactgagcgt accgggaaat ctcaatcttt taggctttgt
    23821 gacgctcatt tacggcattg ggcaaatcct cggcccggcg ctgaccagta tgctcggcaa
    23881 tggaacatcg gcgcttgcca gcgccactct ctgcggcgcg gcggcgttgt ttattgctgc
    23941 gcttatcagc acagtgcaat tatttaagct acaagtggtc acttcatgag ggaagtttcc
    24001 cttaccactc aagaaactca accgcaaccg ttgatgtcag ccagaccccg ttttccgctt
    24061 gccagaaagg gaggcctcgt ttcgccattt cctgtgcttt gacggttaaa ataaccggcg
    24121 atccgtgtct tgctcccact ttgcgtgccg ttgcttcatc agcagagaga tgtacgtagt
    24181 gacgctcacc tgcaatcagc ccctgttttt ttatctcgtc aaggaaacgg cttgccgtac
    24241 cgtggtagag aaactgtggc ggtgtttttt cggcaaagga aatagcgacc tgcgaagtcg
    24301 aatgcccctg aaccgcgcgg atgcacctac catcactgga ataactaaaa cgctttttat
    24361 cgctggtggc aaccacagta tccagcaatg cacgggttaa ccgcttgccc gctttttggg
    24421 cacaaagtat gagcttatcg atatccgccc atccttcacg gtccagtacg ataccaatcg
    24481 cctccggttt gtgtcggagg acaaaactta aaaacttgct cgtatccgcc agctcttttt
    24541 cgttgtactt tgccatcgcg ttttcgtccc tggattatct gttttttatt tactatgtca
    24601 ttgtcactgg gtttatgaat tttctgcaac tcaatcctgc gtagaacctt ttccacacag
    24661 cgtaatttta ccgctatgca aaaaacgcat agtcagaaac atacttgcat tttccccata
    24721 cccacatggc tgataaggtt tttcagctcc aggaattttc ctggctcaag caacacacaa
    24781 acacaaaaaa acaatgggga ttgttatgac acaacaaggg gatgctgtag ccggggagct
    24841 ggcgacagaa aaagtgggca ttaaagggta tctggctttt tttctcacca taatcttttt
    24901 ttccggtgtt ttttccggga cagacagttg gtggcgcgtt tttgattttt ccgttctgaa
    24961 cggatcgttt gggcaactgc ctggtgcgaa cggagccacc acctcgtttc gcggcgcggg
    25021 tggcgcgggg gctaaagatg gctttctctt tgcgctggag ctggcaccgt cagtcattct
    25081 ctctctgggg attatttcaa ttaccgatgg tcttggcgga ttacgcgccg cgcagcagtt
    25141 aatgacgcca gtgctaaaac cgttgttggg cattccaggg atctgctcgc tggcgctgat
    25201 cgctaacctg caaaacaccg atgccgccgc aggtatgacc aaagagctgg cgcaggaagg
    25261 tgaaattacc gaacgcgata aagtgatttt cgccgcatat caaaccagcg gtagcgccat
    25321 tatcaccaac tacttctctt ccggcgtcgc ggtgtttgcc tttctgggca cgtccgtaat
    25381 cgtaccttta gcagtcatcc tggtgtttaa atttgtcgga gccaacattt tgcgcgtctg
    25441 gcttaacttc gaagaacgtc gcaacccaac gcaaggagcg caagcatgac aactcaggtc
    25501 cgcaaaaacg tcatggatat gtttatcgat ggtgcgcgcc gtggctttac tatcgccacg
    25561 accaacctgc tgccaaacgt ggtgatggcg tttgtcatca ttcaggcgct gaaaattacc
    25621 ggcctgctcg actgggtggg gcatatctgt gaacccgtaa tggcgctgtg gggattaccc
    25681 ggtgaagcgg caaccgtcct tttagccgca ttaatgagta tgggtggcgc agtcggtgtt
    25741 gccgccagtc tggcaaccgc tggcgcatta accggtcacg atgtcaccgt tttactgccc
    25801 gcgatgtatt taatgggcaa cccggtgcaa aatgtcggtc gctgcctggg gacagccgaa
    25861 gtgaatgcca aatattaccc gcatatcatt acggtctgcg tgattaacgc attgctgtcg
    25921 atctgggtta tgcagcttat tgtttaacaa ggagttacca tgattgatta taccgcagcc
    25981 ggttttaccc tgctgcaggg agcgcatttg tatgcgccgg aagatcgggg aatttgcgat
    26041 gtcctcgtcg ctaacggcaa aattatcgcc gttgccagta atatcccttc tgacattgta
    26101 ccggactgca cggttgtcga tctcagcggg cagatcctct gcccaggttt tattgatcaa
    26161 cacgtccatt tgattggcgg tggcggcgaa gcaggtccca cgacgcgcac gccggaagtg
    26221 gcgttaagtc gtctgacgga agcaggcgtc acgtcagtgg ttggtctgct gggcaccgac
    26281 tctatctctc gccacccgga atccctgctc gccaagaccc gtgcgctcaa tgaagaaggc
    26341 atcagcgcct ggatgctgac cggcgcttat catgtccctt cccgcactat tacgggttcc
    26401 gtggaaaaag acgtggcgat tatcgatcgc gtgattggcg tgaaatgcgc catctctgat
    26461 caccgttctg ccgcaccgga cgtttatcac ctggctaata tggcggcgga atcccgcgtt
    26521 ggcggtttgc tcggcggtaa acctggcgtc accgtgttcc acatgggcga cagcaaaaag
    26581 gcgttacagc ctgtctatga cctgctggaa aactgcgatg tgccgattag caagttgctg
    26641 ccgacccacg ttaaccgcaa cgttccgttg tttgagcagg cgctggagtt cgcgcgcaaa
    26701 ggcggcacca tcgatatcac cagcagcatt gacgaaccag tcgcccctgc cgaaggtatt
    26761 gcccgcgccg ttcaggcggg tattccgctg gcacgcgtca ccctcagctc cgacggcaac
    26821 ggtagccagc cgttcttcga tgacgaaggg aatttaaccc atatcggtgt tgccggtttt
    26881 gaaacgttgc tggaaaccgt gcaggtgctg gtcaaagatt atgatttcag tatcagcgat
    26941 gccctgcgcc cgctcaccag tagcgtagcc ggtttcctta acctgagcgg gaaaggcgaa
    27001 attctgccag gcaatgacgc agacttactg gtcatgacgc cagaattacg cattgagcag
    27061 gtatacgctc gcggcaagct gatggtcaaa gacggcaaag cctgcgtgaa aggaacgttt
    27121 gaaacggcat aaacggttgt cagccgcccg aatggacgta gaatgcccgc aagggcggct
    27181 gacagagtaa aacgtaatgg atgactgtgg tgcggttttg cataatattg aaactaaatg
    27241 gctttatgat tttctgaccc tggaaaaatg ccgcaatttt tcccaggcag cagtcagtcg
    27301 caacgtctcg caaccggcat tcagccgccg catccgtgcg ctggaacagg cgattggcgt
    27361 tgaattgttt aaccgccagg tgacgccgct gcaactctcg gaacaaggta aaatattcca
    27421 ttcgcagatc cgccatctgt tgcaacagtt agagagcaac cttgcagaac tccgtggtgg
    27481 cagcgattac gcgcaacgca aaattaaaat agccgctgca cactctcttt ccctcgggct
    27541 gttaccgtcc attatcagcc agatgccgcc gctctttacc tgggcaattg aagctattga
    27601 tgtcgatgaa gcggtcgata aactgcgtga agggcaaagt gactgtattt tttcctttca
    27661 cgacgaagat ttgctggaag cgccgtttga ccacattcgc ttatttgaat ctcaattgtt
    27721 ccctgtctgc gccagtgacg aacacggaga agcacttttt aacctcgcgc agccacactt
    27781 tccgttactg aattacagcc gcaactccta catggggcga ttgattaatc gcaccctgac
    27841 gcgccacagt gagttaagtt tcagcacctt ttttgtctct tcgatgagcg agcttttaaa
    27901 gcaggttgcc ctcgacggct gtgggattgc ctggctgccg gagtacgcca tccaacaaga
    27961 aattcgcagc gggcagctcg ttgtactgaa tcgggacgaa ctggtgatcc cgattcaggc
    28021 ctatgcatac cgaatgaata cccgcatgaa tccagttgcc gaacgcttct ggcgtgaact
    28081 gcgcgagctg gagattgtac ttagctgaca ttctcgagcg tcgcactacg ccaaccgcca
    28141 ttatcccaga ccagatcaaa cgttagcggt tcatcgtcgt ggtacagcat tatctcaagg
    28201 ctgatcgcca gcgtgtggct gtacgcttct ttcagattga cctgcaacgc cgcaatccgt
    28261 ggctcatgaa ataacagtgc agaacgcagc tgacggcaat accagctctg gagatgtttg
    28321 tcgctctggt tatgcacttg ccactcgggc atgcctgaaa caagtaatga cggtaatgcc
    28381 gaatgaggag aattaaaaag agtgaggaga tcgctacata ttgatttacg cagcgatgac
    28441 gttttattgc ctgaaatttc aggtaaaaca gcctgacgtg attggcgcat cattttgcgc
    28501 actcctgacg tttagcaaca tatgcggctt gccctgaaca ccgggcacca ctgtattgat
    28561 gctcattttc catagccatt cccttttgtt cattacatcc atgtcagtct gagacaggcc
    28621 tgaatcctgt cagcaaagta taaaacctta cttgcctatg aatatctact gacttttcag
    28681 gaaaaaagtc atgatttgat aaaaatcatc aaactcaaaa aaaaaccgct agtatattct
    28741 gaatattccg aatagttacg tagatgaaaa tacttgtcac caccgctact taattccggt
    28801 gtgaa
//
LOCUS       DAPHBJ010000039        28236 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.039, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000039 DAPHBJ010000000
VERSION     DAPHBJ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.039"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1545)
                     /gene="ycjM"
                     /locus_tag="RHZ76_001554"
     CDS             complement(<1..1545)
                     /gene="ycjM"
                     /locus_tag="RHZ76_001554"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HDV2812211.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTINSSVMRIQRSN
                     ADGNCLT"
     gene            complement(1758..2072)
                     /gene="pspE"
                     /locus_tag="RHZ76_001555"
     CDS             complement(1758..2072)
                     /gene="pspE"
                     /locus_tag="RHZ76_001555"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HDV2812212.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(2147..2368)
                     /gene="pspD"
                     /locus_tag="RHZ76_001556"
     CDS             complement(2147..2368)
                     /gene="pspD"
                     /locus_tag="RHZ76_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HDV2812213.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(2377..2736)
                     /gene="pspC"
                     /locus_tag="RHZ76_001557"
     CDS             complement(2377..2736)
                     /gene="pspC"
                     /locus_tag="RHZ76_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HDV2812214.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2736..2960)
                     /gene="pspB"
                     /locus_tag="RHZ76_001558"
     CDS             complement(2736..2960)
                     /gene="pspB"
                     /locus_tag="RHZ76_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HDV2812215.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(3014..3682)
                     /gene="pspA"
                     /locus_tag="RHZ76_001559"
     CDS             complement(3014..3682)
                     /gene="pspA"
                     /locus_tag="RHZ76_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HDV2812216.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3849..4826
                     /gene="pspF"
                     /locus_tag="RHZ76_001560"
     CDS             3849..4826
                     /gene="pspF"
                     /locus_tag="RHZ76_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HDV2812217.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4946..6211)
                     /gene="puuE"
                     /locus_tag="RHZ76_001561"
     CDS             complement(4946..6211)
                     /gene="puuE"
                     /locus_tag="RHZ76_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HDV2812218.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAVEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(6249..7529)
                     /gene="puuB"
                     /locus_tag="RHZ76_001562"
     CDS             complement(6249..7529)
                     /gene="puuB"
                     /locus_tag="RHZ76_001562"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HDV2812219.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKNYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERHGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTGRLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7531..9018)
                     /gene="puuC"
                     /locus_tag="RHZ76_001563"
     CDS             complement(7531..9018)
                     /gene="puuC"
                     /locus_tag="RHZ76_001563"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HDV2812220.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAMSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(9293..9850)
                     /gene="puuR"
                     /locus_tag="RHZ76_001564"
     CDS             complement(9293..9850)
                     /gene="puuR"
                     /locus_tag="RHZ76_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HDV2812221.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9877..10641)
                     /gene="puuD"
                     /locus_tag="RHZ76_001565"
     CDS             complement(9877..10641)
                     /gene="puuD"
                     /locus_tag="RHZ76_001565"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HDV2812222.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            10853..12271
                     /gene="puuA"
                     /locus_tag="RHZ76_001566"
     CDS             10853..12271
                     /gene="puuA"
                     /locus_tag="RHZ76_001566"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HDV2812223.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVASLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQDGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            12420..12584
                     /locus_tag="RHZ76_001567"
     CDS             12420..12584
                     /locus_tag="RHZ76_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812224.1"
                     /translation="MPMIKSPHGEGGCVFAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12574..13959
                     /gene="puuP"
                     /locus_tag="RHZ76_001568"
     CDS             12574..13959
                     /gene="puuP"
                     /locus_tag="RHZ76_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HDV2812225.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKEHFHYLLMPLVGALTVGVLWINLEATSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            14093..14338
                     /locus_tag="RHZ76_001569"
     CDS             14093..14338
                     /locus_tag="RHZ76_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HDV2812226.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14650..16293
                     /gene="sapA"
                     /locus_tag="RHZ76_001570"
     CDS             14650..16293
                     /gene="sapA"
                     /locus_tag="RHZ76_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HDV2812227.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNIAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKSLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            16290..17255
                     /gene="sapB"
                     /locus_tag="RHZ76_001571"
     CDS             16290..17255
                     /gene="sapB"
                     /locus_tag="RHZ76_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HDV2812228.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            17242..18132
                     /gene="sapC"
                     /locus_tag="RHZ76_001572"
     CDS             17242..18132
                     /gene="sapC"
                     /locus_tag="RHZ76_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HDV2812229.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            18132..19124
                     /gene="sapD"
                     /locus_tag="RHZ76_001573"
     CDS             18132..19124
                     /gene="sapD"
                     /locus_tag="RHZ76_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HDV2812230.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            19126..19932
                     /gene="sapF"
                     /locus_tag="RHZ76_001574"
     CDS             19126..19932
                     /gene="sapF"
                     /locus_tag="RHZ76_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HDV2812231.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            20000..20353
                     /locus_tag="RHZ76_001575"
     CDS             20000..20353
                     /locus_tag="RHZ76_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HDV2812232.1"
                     /translation="MMDKIKSNARDLRSNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDSRRTSWLESQGWTVLRFWNNEIDCNEETV
                     LENILQELNRRSPSP"
     gene            20722..21510
                     /gene="fabI"
                     /locus_tag="RHZ76_001576"
     CDS             20722..21510
                     /gene="fabI"
                     /locus_tag="RHZ76_001576"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDV2812233.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21654..22781
                     /locus_tag="RHZ76_001577"
     CDS             21654..22781
                     /locus_tag="RHZ76_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HDV2812234.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISALVRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22849..24783
                     /gene="rnb"
                     /locus_tag="RHZ76_001578"
     CDS             22849..24783
                     /gene="rnb"
                     /locus_tag="RHZ76_001578"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HDV2812235.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDEMVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24860..24963)
                     /locus_tag="RHZ76_001579"
                     /pseudo
     CDS             complement(24860..24963)
                     /locus_tag="RHZ76_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25017..27002
                     /gene="pdeR"
                     /locus_tag="RHZ76_001580"
     CDS             25017..27002
                     /gene="pdeR"
                     /locus_tag="RHZ76_001580"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDV2812236.1"
                     /translation="MKTVRESTTLYNFLGSHNSYWRLTESSDVLRFSTTETTEPERML
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            27149..27322
                     /locus_tag="RHZ76_001581"
     CDS             27149..27322
                     /locus_tag="RHZ76_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812237.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            27412..28161
                     /locus_tag="RHZ76_001582"
     CDS             27412..28161
                     /locus_tag="RHZ76_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV2812238.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
BASE COUNT         6545 a         7312 c         7239 g         7140 t
ORIGIN      
        1 agtcagacaa ttaccatcag cgttacttct ttgaatacgc attacggatg aattaatcgt
       61 atcaatggta aaattattat ccggatgaaa ctcgttatgg ctgcgacgaa gtgtaattaa
      121 acgcgacaac tcatgatata ccgcatgcct taatgtagct tcatcgttca gttctcgggt
      181 tatctcttta ctgtgatatt ttttacggtt aatcgcacgg ttatatccga gtttttcgac
      241 acctgcgtaa tcattacgcg agccaagaat actttgaata tatatcgctg gcacaccggg
      301 gaaacttaac aaaatcgcat gggcgagaat aaacctggcg caacgttctt catcgctact
      361 ctcacgccgg cttaacgcat ccatataggt cacatttatt tcatacggac tgcgtgtacc
      421 gtcgggatta tttttccagt ttactaatgc accttcctgc tgtaacgcct cgaccagctc
      481 taatatttcg ctttcaggca acaagccccg tagcgggttt agcccgatgc catcgtgaga
      541 ggcgaggaag ttaaaccagg tggtgttgct ggaaggtagt gtcaggtttt gcgcccacgc
      601 acaaagcgcc tcaacgttct gtttttgcac cgcatgcagc accagtggcg gcagcgagaa
      661 ctggtacacc atatgtgctt cgtcatcgcc tgcgccaaag taagcaatgt tgtctttatg
      721 cggaacattg gtctcggtaa tgatcactgt acctggcgca acgttatcaa taatcgaccg
      781 taacagtttg ataatcagat gtgttttttc cagatggatg cagcttgttc ccggctcttt
      841 ccacataaag ccaacggcat ccaggcggac atactcagca cctttcgcaa ggtaacagag
      901 taaaacatcc accatcgcca gcaacacttc agggctacgg taattcaggt caatttgatc
      961 gtcactaaag gtggtccata aatggcgcgt tgaatgatcg cgcatctgga atggcgttaa
     1021 taacggtaac gcacgcgggc gagtgacggc gctgagatcg gtttgcgggt caacggcaat
     1081 aaaaaaatct tcaaaacctg gatgctgttg taaatagttt ttaaaccatt cactttttgc
     1141 cgacatatgg ttgcagacaa aatcaaacat taaatgactg cattcaccga gttgctgaat
     1201 atcctgccac tcccccgctt cactggcgac ctgatgataa tcaattaccg aaaagccatc
     1261 atcagatgac catggataaa acggcaacaa atgaacatgt gaaaaaatgc tttgcagcca
     1321 ttgatggtaa aactgattaa atgtgggtaa tggttttaaa tcattgctgt gaaattgatc
     1381 ggcataggta attaacacga catcactttc atcccagtgt tttttacgtc gctgtgtaat
     1441 taatcgtttc gcactctcaa gacgcgttac aagtttctgt aaatgagttg cggtaaatgt
     1501 tccaccgtag atttcgtcca ggtaatccgt aattttctgt ttcatgttgc ctttccgtgg
     1561 tagcggtccc atgaaacgag agactactcc gcagaaaata aactgtcaat tgagcacaag
     1621 ggttttagtc acgcaggaaa tcatttttta ccagggaaaa agcgtatttg tgagctgacg
     1681 caaataatct atggggatgt aaataaggtg tgacagaaaa aaaaacggcg cataagcgcc
     1741 gctcatggtg aattctttta acctttgacc ttcggcattg cgatgtcttt caggccaccg
     1801 gcgttctcaa cgtgggtata tcccatctcg ctaaggatct cttttgcttg ccctgactgg
     1861 cgtccggcat tgcaatacac tttcacggtg tcgtttttat ccggaacggc ggtggcaatg
     1921 cgctctttca cttccttcag gggaatattg atggccccct gaacgtgctc ttgctgatac
     1981 tgctctggaa cacgaacatc gatccagtgt tcagcggcga aaacgggcag tgaaaacacc
     2041 agtgccagag caagtaagcc ttttttaaac atgtaagcgt ccttctatgg atgaaaattg
     2101 ccatccatta tacgcctgag atttaacaat ttttgcgaaa ctcccctcac cttttataac
     2161 gctgtgccag tttattagca gcccgactta acagcggttc cagcgccaca gccagcaaca
     2221 ttttcagcgg tcggcgagca actgatttta tcgcccagcc cgccacaccc gccgggccat
     2281 agcgcagtgc ggtaagaagt accagcttgc ctgctaattt gaaaccaggc tttacctttt
     2341 gcccggcctg ttgccagcga gtattcataa ctttcctcac agttgacgga aacggctacg
     2401 taacgtgaaa gtatcggaag tgacataacg ttccatctcg cgtaaacgcg tttcacttgc
     2461 cgccagttca cggtcgactt catccagcaa ttcgctgctg gaaggtagct gctcaccaaa
     2521 ggccatgttg tccggcattg gatcaagcgc aaatgacaaa atgatgtaag caaccagggt
     2581 aaacagcgcc agaccgaaga aaatcgacag caccaccagg atacgcacca gttttaccgg
     2641 tacatcaaaa tagttggcaa tcccggcgca gacgccgcgg accatgccct gctgtggaat
     2701 acgccataat tttttattga gattaatgcc cgccattagc gatccctcca gttcggatgt
     2761 tctgcatcaa gaatagattc cagcgcctga atacgttcgc gcatccgttt tgcttcatca
     2821 gccagttgcg ctaatcgctg ctgctcactt tgcgacaatt cactgcgacc agaacgattg
     2881 ctgtaatgca gccataacca gatcggtaaa acaaacagca caaaaatggt taacggaata
     2941 gccagaaata gcgcgctcat aagtactcct tagacgatga gcggcggcgc gttcggacgc
     3001 cgcctggatg ttattattga ttgtcttgct tcattttggc ttttaattgt gccagttgtt
     3061 cgctgattgc atcatcggct ttcagttcgg caaactgatc gtccagcgat ttttgtttac
     3121 cgaagctgtg gctttctgct tccgcttcca tctggtcaat acgacgttcg aaagattcga
     3181 aacgagccat tgcttcatcc agtttgccac tgtccagctg acgacgcaca tcgcgcgacg
     3241 agtttgccgc ctgatgacgt aacatcaatg cctgctggcg agcgcgtgtt tcgctcaatt
     3301 tgttttccag ctcgccaatc tctttcttca tgcgtgccag cgtatcgtcc accagcgtca
     3361 cttcatgttc cagggactta atcagatcgg tcagtttctg tttttcaatt aacgctgcac
     3421 gtgccagatc ctctctctct ttcagcagcg ccagttcggc tttttcctgc cattcaacct
     3481 cacgcgccga cgcttgttca atacggcgag tcagctgttt cttttctgcc aacgcgcgcg
     3541 ccgaagtaga acgtacttca accagtgtat cttccatctc ctggatcatc agacgaacca
     3601 gtttctgtgg atcttccgct ttctctaaca gagcgttgat gttggcattc acgatgtcgg
     3661 caaagcgaga aaaaataccc ataatgttgt cctcttgatt tctgcgttca ggattgtcct
     3721 gctgaactgt taatacaatt tgcgtgccaa ttttttatct ttttgattta taaagatctg
     3781 attgaagaat caacagcaac atgccaggat gagttagcga attacactaa caagtggcga
     3841 atttcatcat ggcagaatac aaagataatt tacttggtga ggcgaacagc tttctcgaag
     3901 tgctggaaca ggtttcgcat ctcgcaccgc tggacaaacc ggtgctcatc atcggcgaac
     3961 gcggcaccgg taaagagctg attgccagcc gcctgcatta tctctcctcc cgttggcaag
     4021 ggccgtttat ttcccttaac tgcgcggcgt taaatgaaaa tctgctggat tccgaactgt
     4081 ttggtcacga agcgggggcg tttaccggtg cgcaaaaacg tcatccaggg agatttgaac
     4141 gtgccgacgg cggtacgcta tttcttgatg aactcgctac ggcaccgatg atggtgcagg
     4201 agaaattatt gcgcgtgatt gagtacggtg aactggagcg cgttggcggc agccagccat
     4261 tgcaggtgaa tgtgcggttg gtatgcgcga cgaatgccga tctcccggcg atggtcaatg
     4321 aaggcacttt tcgcgctgac ctgctcgacc gactggcttt tgatgttgta caactgccac
     4381 cactgcgcga gcgcgaaagc gacataatgt tgatggcaga acactttgcc atccagatgt
     4441 gtcgggaaat caagctgcct ctgttcccgg ggtttacgga gcgcgccaga gaaacattgc
     4501 tgaattatcg ttggccggga aatattcgtg aattgaaaaa cgtggtggaa cgttcagtgt
     4561 atcgccacgg caccagcgat tatccgcttg atgacatcat tattgatccc tttaaacggc
     4621 gtccgcctga agacgctatc gccgtttcag aaaccacctc gcttccaaca ctgccgctgg
     4681 atttacgtga gtttcagatg cagcaggaaa aagagttgct gcaactcagt ttgcaacaag
     4741 ggaaatataa ccagaagcgc gcggctgaat tactggggtt aacttatcat cagttccgcg
     4801 cgttgttgaa aaagcaccag atttagcgaa gttgagcaat acaacttgcc ggatgcggcg
     4861 tgaatgcttt attcggcata cataatcaat acgtcgtttg caggcctgat aagcgcagcg
     4921 catcagacat tattgcgttg ggctattaat cgctcagcgc atcctgcaaa attttcattg
     4981 ccgcatcgaa ttgcgcatcc gggatggtca gcggatacag gaagcgaatc acgttgccgt
     5041 atgcgccaca ggtcagcagg agcagcccct gcgccagcgc gcgttgctgg attttctgtg
     5101 caatcgccgc tgacggctcg cccgtttgcg gatcgttaaa ctctaccgca atcatcgacc
     5161 ccaggccgcg taccgcagca atggccggaa cgctttcttt ggcatcaatc aacgtgtttt
     5221 tgagacgctg gcccagttga ttcgcgcgtt cgcagagtga ttctttgtcg ataatattga
     5281 gcaccgcgtg cgcggcagcc accgccagcg ggttaccggc gtaggtgccg ccaagcccgc
     5341 ccggcgcggg tgcgtccata atattcgcgt taccgaccac gcccgaaagc ggcatcccgc
     5401 ccgcgaggct tttcgccatc gtcattaaat ccggcttatc ggcgtaatga tccatggcaa
     5461 acagcttacc ggtacgcgca aagccgcttt gcacttcatc agcaatcatc acaataccgt
     5521 gctcgtcgca caggcggcga atagcggcaa ccagctcttt tggcgcaacg ttgaaaccgc
     5581 cctcgccctg caccggttcg aaaataatcg ccgccacctg cttcgcttcg atgtctgatt
     5641 taaacaagcg ttcgatggcg tcgagggagt cctgtgttga aatgccgtgt aaatctgacg
     5701 gataaggtac gtgatacacc gaaccaggga acgggccgaa gccgattttg tacggcgcaa
     5761 cttttccggt cagcgccatg gtcatatacg tacgaccgtg aaagccgccg ctaaacgcaa
     5821 tcacgccagg gcgtccggta tgggcgcgag caattttcac cgcgttttcc accgcttccg
     5881 caccggtggt gaagaacgcg gttttggcct gcccgctcac cggggcaagg gcgttgattt
     5941 tctccgccag ggtgacgtag ctttcatacg gcacaatctg atacgcggtg tgggtaaact
     6001 gttgcagttg ctgctccacc gccgcgacca gatcagggtg gcgatgtccg gtattcagca
     6061 ccgcaatgcc tgcggcgaaa tcgatgtact cgttgccctc aacatccttc agcgtggcgt
     6121 tttcagccga ctgggcgaag aagttacaca tcacgccaac cccgcgcggg gtggcagaaa
     6181 gacgacgctg atggaattca ttgttgctca tgtttcacac cttttgactc ttcacggtta
     6241 atcggaaatt aaacgcccag acgatcgcgc aggctgtaat acgccgcgcc catcgcggta
     6301 aacggcacac gcagcgtgcg cccgccgggg aacgggtaat gcggcagatt ggcgaaggca
     6361 tcgaaacgtt cggcgtcgcc gcgcagcagt tcggcaatca aacgtccggc tagatgagta
     6421 caggtcacgc cgtggccgct gtagccctgc atgtaataga tgttggtatc gaggcgacca
     6481 aactgcggca tacgcgacag ggtcagcagg aagttgcccg tccagcggta atcaattttc
     6541 acgcccttca gctgcgggaa ggttttcagc agtttcggca ccacaaggcg ctcaacgtca
     6601 tccgggtcgc gcgcgccgta gaccacgccg ccgccgtaca gcaggcggtt gtcggcggta
     6661 agacggtagt aatcaagcag atagttacag tcttccacac agtagttttt cgggatcagc
     6721 gaacgggcta aatcttccga cagccgtccg gtggtgatca cctgcgtgcc gcacggcatg
     6781 ctgcgtttcg ccagttccgg ctctacttta tcgcccagat acgcattccc ggcgacaatc
     6841 acatacttcg ccgtcacctg acctttggca gttcgcacaa cggctggcgt ggtgtgctgg
     6901 atctgcgtca cggcagaaag ttcatacacg cgcccgccgt tgaggcggat ggcgtccgct
     6961 tcgccaatgg cgaggtttag cggatgaata tgcccaccgc tgtgatccag cagcgcacca
     7021 gtgtagcgat cgctggcgac ctcgcggcga atagcgttgg cgtccagcag ctccagctgt
     7081 ttattgccgt ggcgttccca gttctctttc tgctcttcga gtgtggcgag ttgtttatca
     7141 ttcatcgcca caaacagacc gccgggacga tagtcgcagt caatctgata acgtttgata
     7201 cgttcgcgga tgatctcgcc accctcgaac atcatgctgc cgagcatacg ggcggtgtcc
     7261 atgccgtagt ttttttcgat cacgtcgatg tcgcggctgt aggagttcac aagctgcccg
     7321 ccattgcgtc cgcttgcgcc gaagccgatg cgtgaggctt caagaaccac cacgtcaaag
     7381 cccgcttccg ccagatgcag cgcggaagag agcccggtat agccgccgcc gaccacgcaa
     7441 acgtcgcagg aaatcgactc attcagcgtg tcgaatggcg cgtatttatt cgcactggcg
     7501 gcgtagtaac tgctggtatg ttcggtcatt tcaggcctcc aggcttatcc agatggtttt
     7561 cagttcggtg aatttttcaa gggcatgcag ggatttgtcg cgaccgttgc cgctctgctt
     7621 atagccgcca aacggcacgg tcatatcgcc gtcgttgtag ttattgacga agacggaacc
     7681 agccttcagg cggcggctca tgcggtgcgc gcgggagagg tcgcgcgtcc ataccgccgc
     7741 gccaaggccg tactggctgt cgttggcaag ctgtagcgcc tgttcttctg atgtgaaacg
     7801 cgtgaccacc agcaccggac cgaaaatctc ttcgcggctt aaggacgcat tcgggtctac
     7861 atccacaaag atggtcgggc cgatggcggc agccagcccg gcgttacggc catccaacaa
     7921 cagttgccct ttgctttcgc cttcccgaat aaagctatgg accgagtcgg cgtgggcgca
     7981 gtcgattaag gtgcccatgg tggttgcggg atcaagtgga tggcccggct gccagttttg
     8041 cgcctgctgt tttaacaggg ctaagaattc atcggcgatg ctctcttcca gcaacaggcg
     8101 cgttccggcg atgcacacct gtccctggtt gtagaaaatg cctgctgcgg tggcgcttgc
     8161 cgcctgttgc aaatccgggc agtcagcgaa aacgatgttg gcgcttttgc cgcccgcttc
     8221 cagccagacg cgtttcatgt tgctgtcgcc cgcatctttc agcagctgtt tcccggtacg
     8281 ggttgaaccg gtaaaggcaa tggcgtcgat atcgttatga cgcgacagcg cctgcccggc
     8341 ttcatgacca aaacccgtca ccacgttcaa cacaccatcc ggcaagcctg cttctttcgc
     8401 cagccccgcg agacgaatcg cactgagcgg tgatttttca gacggtttta gaatcacgct
     8461 gtttcccgcc gccagcgccg ggccgagttt ccagcaagtc agcagcagcg ggaagttcca
     8521 cggcacgatg gcggcaatca cgccgaccgg ttcacgcacg atcatcgcca gctcatggct
     8581 actggtggtc gccacttcgc catacacttt gtcgatcgct tcggcgtacc agcgaatggc
     8641 gcgcgccgcg ccgggaatat catcacgcag actgtgacga atcggtttgc cggtgtcgag
     8701 agtttccagc agtgccagct cttcggcgtg ggcttccatt aaatcggcga gtttattcag
     8761 taccgcttta cgtttagccg gagaagagag tgaccagtcg ccgcgttcaa atacgccgcg
     8821 tgctgcgctc atcgcacggt cgatatcgac gctcttgccg cgggcaattt tcgccagcgg
     8881 tgcctgggtg accggatcaa cggtttcaaa ggtttcattt tccgccgcag cagtatattc
     8941 accgttaata aataagcggt tttcaatggc gagacttaac gctttatcct gccagtaagc
     9001 cagatgatga aaattcatta tgactcctgt ttcacgtcta tcagatatat gcagtttgtg
     9061 aacgtcaggg cgcagagatc cccgggccgt atgcccgttt atttctggcg acattcttta
     9121 tatacacttc atccttcaag ccgcctctgc cttggctgcg ttcgctcccc cgaatcactt
     9181 acctgagtaa gctcatcggg attcgctcac ttgccgcctt gatgcaactt gaatgatttt
     9241 gtgtatagat tgccgggcgc attgcgctta cccggcttac agaacaaaaa gattaaaacg
     9301 tggtgggcgt atgggcgctg ataattcggc aaatacctgc cgacgtatta ctgaaactgt
     9361 gcgggatgcc ggtattaatg gcataacttt gccccgcgac gaggtggtaa tcctgaccat
     9421 taatcgtcag aacaatttca ccttccagta cagtgcctat ttcctcaccc tgatgcttaa
     9481 ttctttcccc agtggttgtg cccggctggt acgtttcaaa gatcatcgcc agcgtgcgat
     9541 tcgggttacc gttatgaacc agcttcattg acacaccctg actacccatc tcaattaagt
     9601 cgtcctgatt aatgacgacc tgcggctcat caggtttttc cggctcggaa aagaattccg
     9661 agagtgacag accatacacc ttcagcagct tttgcagcgt actgatggca gggctgactt
     9721 tatcttgttc tatcgtactg atagcactgt gagtcagccc ggagagttcg gcggcacgac
     9781 gttgtgaaag cccctgctgc tggcggattt ccgacaagcg ttttcctggc gccagtccct
     9841 catcactcat atttgcattt ccttaaactg tagtggtcag agtcgctgtt tttcagcgat
     9901 atggtgctga caagcggtga taaagccctc gaacaatata cgcgaaagcg cgtactcgct
     9961 actgttccat tccgggtgcc actgtacgcc cagcgcaaaa ggatgattga tgacgctaac
    10021 cgcctcaact aagccatccg gcgagcgagc ttcaacacgc aaccgtgggc taacgacctt
    10081 cgccccttgt ccatgtagag agtttaccca aaagttgcta cattcaggta acaacgcaga
    10141 cagtaatccc ccctcttcaa cctgaacttc atgcgacggt gcatattgct gttccaccgg
    10201 cagttcggga tcttcccgat gttccagcaa ttcaggctgt tcgcacagct tgcgatgcaa
    10261 cgaaccaccg gttgccacca ccagttcttg taaaccccgg cagatggcga aaatggggat
    10321 gcgcctttcg agtgcggcat taattatcgc catgctcaga agatcacgcc cgggatcggc
    10381 gtcaggctca tcgccgtttt caccatatag gtgcggctgc acattgctgg gactaccagg
    10441 aagataaatg ccatcgagtt tcggcaaaag ttgttcaagt aatgacggtt ccgccagcgc
    10501 atgtggtagc gcaataggca agccgcctgc atggatgatg gcattcaggt acttttcttg
    10561 cagagtctgg gtcgcatgac ccttaagcct gttcctgcac attacgacac cgataaccgg
    10621 attgttcatt atattttcca tgctcaatct cacaaagtgg actaaattat cgccattact
    10681 gcgctttaaa tgacctttat gttcaatatt ttttcaatct agcagtgggt tttcattttt
    10741 gcaaactcaa tttaacattt gacaaacatt tagtttgcat acagattcga atggtggtca
    10801 ttatatttta cgctttgata acgagcggaa aacaaaccaa aggcgaagaa tcatggaaac
    10861 caatatcgtt gaagtagaga actttgttca gcagtcagaa gagaggcggg gtagcgcctt
    10921 tacgcaggaa gtgaaacgct acctggagcg ctacccgaat acgcaatatg ttgatgtttt
    10981 acttactgat ttgaatggtt gcttccgtgg aaaacgtatc cctgttgcaa gcctgaagaa
    11041 gttggagaaa gggtgttatt tcccggcctc ggtgtttgca atggatattc tgggcaacgt
    11101 agtagaagaa gcgggtctgg gtcaggaaat gggcgagccg gatcgtactt gtgttcctgt
    11161 tctcggttcc ttaacccctt ctgccgccga tccagagttt atcggtcaga tgctcctgac
    11221 catggtcgat gaagatggcg ctccctttga cgttgagccg cggaacgttc ttaaccgcct
    11281 gtggcagcag ctacgccagc gcggattgtt cccggttgta gcggtagagc tggagttcta
    11341 tttactggat cgtcagcgcg acgctgaagg gtatctgcaa ccgccctgcg cgcccggcac
    11401 cgatgaccgc aatacacaaa gccaggttta ctccgttgat aacctcaacc acttcgccga
    11461 cgtgctcaat gatattgatg aactggcgca gttacagtta attcccgccg acggtgcggt
    11521 cgccgaagcc tcgccgggtc agtttgaaat caacctttac cataccgata acgtgctgga
    11581 agcctgcgat gatgcgctgg cgctaaaacg cctggtgcgt ctgatggcag aaaagcataa
    11641 gatgcacgcc acttttatgg cgaagccgta tgaagagcac gcgggcagcg ggatgcatat
    11701 ccatatcagt atgcaaaata atcgtggcga gaacgtgctt tctgacgcgg aaggcgaaga
    11761 ttcgccgctg ctgaaaaaaa tgctcgccgg aatgatcgat ctgatgccgt catcaatggc
    11821 gttgctggca ccaaacgtga actcgtatcg ccgcttccag ccggggatgt atgtaccgac
    11881 gcaggcgtcg tggggccata acaaccgcac cgtcgccctg cgtattccat gcggcgatcg
    11941 tcataatcac cgcgtggaat atcgcgtggc gggtgccgat gccaacccat atctggtgat
    12001 ggcagcgatt tttgccggta ttttgcacgg tcttgataac gagctgccgt tgcaggaaga
    12061 agtcgaaggc aacgggctgg aacaggatgg cttacccttc ccgattcgcc agagcgatgc
    12121 cctgggtgag tttatcgaga atgatcacct gcgccgctat ttaggcgaac ggttttgcca
    12181 tgtctatcac gcctgtaaaa atgatgagct gttgcagttt gagcgcctca tcacagaaac
    12241 agaaattgag tggatgttga aaaacgcgta ataccccttg cggggttgtt tcatcggcct
    12301 gagcggagcc aggcctgaca tttgctggta atccagacat tttccggatg tcgcgttatg
    12361 gagcgcaggc ggcaacgggc ttccgttaac gattaagact gtgacgagga aatgagatga
    12421 tgccgatgat taaatccccg catggcgagg gaggctgcgt attcgcccct cctgcgactg
    12481 actggactcc gccccctttg cttcccttgc taaacaggtt tgatttcagg tcgacacgac
    12541 cgcaaacctt attacgcagg ggaggcagca attatggcta ttaattcacc actgaatatt
    12601 gctgcgcaac ccggcaaaac ccgtctgcga aaatcactga aattgtggca ggtggtaatg
    12661 atgggtctgg cctatctcac gccgatgact gtgttcgata cttttggcat tgtgtcaggc
    12721 attagcgacg gtcacgttcc ggcgtcctat ttgctggcgc tggcgggcgt gctgtttacc
    12781 gctatcagct acggcaaact ggttcgccag tttccggagg ccggttcggc ctatacctac
    12841 gcgcaaaagt cgattaaccc gcacgtcgga tttatggtcg gctggtcatc actgctggat
    12901 tatctctttt tgccgatgat caacgttttg ttggcgaaaa tctatctctc cgccctcttc
    12961 ccggaagtgc cgccgtgggt gtgggtggta accttcgtcg ccattttaac cgccgcgaat
    13021 ctgaagagcg tcaacctggt cgctaacttc aataccctgt ttgtgttggt gcaaatctcc
    13081 atcatggtgg tgtttatctt cctggtggtt cagggactgc ataaaggaga aggcgttggc
    13141 acggtctggt cgttacagcc gtttatcagc gagaacgcgc atctgattcc gattattacc
    13201 ggggcgacga tcgtctgctt ctctttcctt ggtttcgatg cagtgaccac actttcggaa
    13261 gagacaccag acgccgcacg cgtgatcccg aaagccatct tcctgacggc ggtctatggt
    13321 ggcgttatct ttatcgcggc gtcgttcttt atgcagctgt tcttccccga tatcagccgc
    13381 tttaaagacc cggacgccgc actgcctgaa attgcactct acgtcggcgg taagctgttc
    13441 cagtcgattt tcctttgcac cacgtttgtg aacacgttag cgtctggcct ggcctcacat
    13501 gccagcgtgt cacgtctgct gtatgtgatg gggcgcgaca atgtgtttcc ggagcgcgtg
    13561 tttggctatg tgcatccgaa atggcggact ccggcactga acgtcattat ggtcgggatt
    13621 gtcgcgatgt cggcgctgtt cttcgattta gttaccgcca cagcgttaat taacttcggc
    13681 gcgttggtag cgtttacctt cgttaacctg tcggtgttca accacttctg gcggcgcaaa
    13741 ggaatgaata aaagctggaa ggaacacttc cactatttat taatgccgct ggttggcgcg
    13801 ctgacggtgg gcgtgctgtg gattaacctc gaagccacct cgctgacgct gggtctggta
    13861 tgggcttcgt taggcggcgc atatttgtgg tatctgattc gccgctatcg caaagtgccg
    13921 ctgtacgaag gtgacagaac gccagtgagc gaaacgtaat agtcggatgc gcgccgcatt
    13981 atcctgcctg agggtaatgc gccgcgccat taactataaa aagatgtatg acccggatca
    14041 cgagtgctat catccgccct tatgcttagc gcattaaccc caggggcaaa taatgaacca
    14101 cgatatcccg ctaaaatatt ttgatattgc cgatgagtac gcgaccgaat gcgcagagcc
    14161 tgtcgcagac gctgaacgca caccgctggc ccactacttc cagttactgc tcacccgttt
    14221 gatgaacaat gaagagatca gcgaagaagc ccaacatgaa atggctgctg aagcggggat
    14281 taatcccgta cgcattgatg agatcgcaga gtttttgaat caatggggta atgagtaatt
    14341 atttaattgc agacgatcgg tcagtatcgt tgtccggata aagcgttcac tctgcatccg
    14401 gttaaaatga cacgcattgc agcgtgcttt aatgggtttc tcacgatcgt accgctctgc
    14461 tcctcgctaa gttgttctgt gagttatagt tatagagaaa tcacgggtac gaatgttgaa
    14521 cagatggcga cgccatctgt tcaacgacca accacacggc cctcattacg actcctgaac
    14581 acaaacggta atggcaaatg tcattaaata ttagtaagat agcctcttta cataaaatcc
    14641 cttaatatta tgcgccaggt attatcgtct cttttggtga ttgctggact tgtgagtggt
    14701 caggcaatcg ccgcgcctga atctcccccg catgctgata tccgcgacag cggttttgtc
    14761 tattgcgtca gcgggcaagt caacaccttt aacccatcca aagcgagcag tgggttaatt
    14821 gtcgataccc ttgccgccca gttttatgat cgactgctgg atgtcgatcc ctatacctat
    14881 cgcctgatgc cggaacttgc cgaaagctgg gaagtactcg acaacggcgc gacctatcgc
    14941 ttccacctgc gtcgcgatgt tccgtttcaa aaaaccgact ggtttactcc cactcgtaaa
    15001 atgaatgccg acgatgtggt gtttaccttc cagcgaattt ttgaccgcaa caacccgtgg
    15061 cataacgtca acggcagcaa cttcccctac ttcgacagcc tgcaatttgc cgataacgtg
    15121 aaaagcgtcc gcaaactgga taatcatacc gttgagttcc gactcgccca gccggatgct
    15181 tcttttttgt ggcacctcgc aacccattat gcttcggtca tgtcggcaga atatgcccgg
    15241 aagttagaga aagaagatcg ccaggagcaa ctcgaccgtc aaccggtcgg caccggtccg
    15301 tatcagttgt cggaataccg cgccgggcaa tttattcgcc tacaacgtca tgatgacttc
    15361 tggcgcggta aaccgttaat gccgcaggta gtggtggatt taggctccgg cggcactgga
    15421 cgtctgtcga aactcctgac cggggaatgc gacgttctgg cctggcctgc tgccagccag
    15481 ctatccattt tgcgtgacga cccgcgcttg cgtttaacgc tgcgtcctgg gatgaacatc
    15541 gcctatctgg catttaacac cgccaaaccg ccgctaaata atcccgctgt ccgccatgcg
    15601 ctggcactgg cgattaataa ccagcgcctg atgcaatcca tctattatgg tacggctgaa
    15661 acggcggcct ctattttacc gcgcgcctcg tgggcctatg acaacgaggc taaaattact
    15721 gaatacaatc cggcgaaatc gcgcgaacag ttgaagtcgt tggggctgga aaatttaacg
    15781 ctgaaactgt gggtgcccac acgttcgcag gcgtggaacc ccagtccact gaaaactgcc
    15841 gaactgattc aggcggatat ggcgcaggtt ggcgtaaaag tggtgattgt gccggtagaa
    15901 ggtcgctttc aggaggcgcg gttgatggat atgagccatg atctgacgtt atccggttgg
    15961 gcgacggaca gtaacgaccc ggacagtttc ttccgtccgt tactgagctg cgcggcaatt
    16021 cattcgcaga ccaacctcgc ccactggtgc gatccgaaat tcgacagcgt attgcgtaag
    16081 gcgctctctt cgcagcagct ggcggcgcgt attgaagcct atgacgaagc gcaaagtatt
    16141 ctggcgcagg aattgcccat tttgccgctg gcgtcgtcat tgcgtttgca ggcctatcgg
    16201 tacgatatca aaggtctggt acttagcccg tttggtaacg cctcctttgc tggggtgtat
    16261 cgcgagaaac aggatgaggt gaaaaaacca tgattatctt caccttacgc cgcattttgt
    16321 tattgattgt caccttgttc ctgctgacct tcgttggctt cagcttgagc tatttcaccc
    16381 ctcatgcgcc gttacaaggc gcgtcactgt ggaatgcctg ggtgttctgg tttaacggcc
    16441 tgatccactg ggattttggt gtttccagca ttaacggtca accgattgcc gaacagttaa
    16501 aagaggtctt cccggcgacg atggaactgt gcatcctcgc cttcggtttt gcgctgattg
    16561 ttggcatccc cgtggggatg attgccggga ttacgcgcca taagtggcag gataacttga
    16621 tcaacgccat cgccttactg ggcttttcaa tcccggtgtt ctggctggcg cttctgttga
    16681 cgctgttttg ttcactcacg ctaggttggt tgcccgtttc agggcgtttc gatctgctct
    16741 acgaagtgaa accgattacc ggttttgcgt tgattgatgc ctggctttcg gactcaccat
    16801 ggcgggatga aatgatcatg agcgcaatcc gccatatgat attgcccgtg atcaccctgt
    16861 cggttgcgcc aacaactgaa gtgatccgcc tgatgcgtat cagcactatc gaagtgtatg
    16921 accagaacta tgtaaaagcg gcggcgaccc gcggtttgtc gcgctttact attttgcgtc
    16981 gccacgttct gcataacgcg ttaccgccgg ttattcctcg cctgggctta cagttttcca
    17041 ccatgctgac gctggcgatg atcaccgaaa tggtttttag ctggccgggt ttagggcgct
    17101 ggttaattaa cgccatccgc cagcaggatt acgcagccat ttccgccgga gtgatggtgt
    17161 gtggctcact ggtcattatt gttaacgtga tttctgatat tttgggtgcc atggctaacc
    17221 ctctgaaaca taaggaatgg tatgccttac gatagcgtat acagcgaaaa gcgcccgccg
    17281 ggcacgctgc gtaccgcctg gcgcaaattt tatagtgacg cctctgcgat ggtcggcctg
    17341 tacggctgcg cggggctggc tgtactgtgt atttttggcg gctggtttgc gccttacggc
    17401 atcgaccagc aatttctcgg ttatcaatta ttgccgccgt catggtcgcg ctatggcgaa
    17461 gtttctttct tcctggggac tgacgacctc ggacgcgatg ttttaagccg tttactgagc
    17521 ggagctgcgc caaccgtggg tggcgcattt gtggtgacgc ttgccgcgac gatctgtggc
    17581 ctggtactgg gcaccttcgc cggggcgaca cacggcctcc gctcagcggt gcttaaccat
    17641 attctggata ccttactggc gatcccttcg ctgctgttgg caattatcgt tgtagcgttt
    17701 gccggaccga gtttgtccca cgctatgttt gctgtctggc tggcattgct gccgcgtatg
    17761 gtacgttcga tttacagcat ggtgcatgac gaactggaaa aagagtacgt tatcgccgcc
    17821 cgtctggatg gcgcatcaac gctgaatatt ctctggtttg ctgtgatgcc aaacatcacc
    17881 gccgggctgg tcaccgagat cacccgcgca ctgtcgatgg caattctcga tatcgccgcg
    17941 ctgggctttc tcgatctcgg cgcacaactc ccctcgcctg aatggggagc gatgctcggt
    18001 gatgcgctgg aactgattta tgtcgccccg tggactgtca tgctgccagg tgcggcaatt
    18061 atgattagcg tgttgttggt taacctcctt ggcgatggcg ttcgtcgtgc gattattgcg
    18121 ggggtggaat aatgccatta ctcgatattc gtaacctgac cattgaattt aaaaccggtg
    18181 atgagtgggt taaagccgtc gaccgggtaa gtatgacctt aaccgaaggt gaaatccgcg
    18241 gtcttgttgg tgaatccggt tccggcaaga gtttgattgc gaaagcgatt tgtggggtga
    18301 ataaagataa ctggcgtgtt actgctgacc gtatgcgttt tgatgatatc gatttgctgc
    18361 gtctctccgc acgcgaacgg cgcaaactgg ttggccataa cgtgtcgatg attttccagg
    18421 aaccgcagtc gtgtcttgac ccttcagaac gtgtgggccg ccagttgatg caaaacatcc
    18481 cagcctggac ctataaaggc cgttggtggc agcgttttgg ctggcgcaaa cgccgtgcca
    18541 ttgaactgct gcaccgcgtg gggattaaag atcacaaaga tgcgatgcgc agttttccct
    18601 atgagttgac cgaaggtgaa tgtcagaaag tgatgatagc cattgcactg gcgaatcaac
    18661 cgcgtctgct gattgctgac gaaccgacca actcaatgga gccaacaacc caggcgcaaa
    18721 tctttcgcct gctgacgcgt ctcaaccaaa acagcaatac cactattttg cttatcagcc
    18781 atgacttaca aatgcttagc caatgggcgg ataaaattaa cgtgctttac tgcggtcaaa
    18841 cagtggaaac cgcgccgagt aaggagttgg tgacgatgcc acatcatcct tatacccagg
    18901 cgctgatccg cgcgatacca gacttcggca gcgcgatgcc gcataaaagt cgcctcaata
    18961 cgctgcccgg cgctatccca ctgctggaac agttaccgat tggttgtcgt ctggggccac
    19021 gttgcccgta tgcacaacga gaatgcattg tgacgccacg tttgacgggg gcgaaaaatc
    19081 atctctatgc ctgtcatttc ccgctgaaca tggagaaaga gtgagatgat cgaaacgctg
    19141 cttgaagtgc gtaatttaag taaaacgttc cgctaccgga ccggctggtt tcgtcgtcag
    19201 accgtagaag cggtaaaacc cttgagcttt acgctacgtg aaggccagac actggcgatt
    19261 attggcgaga atggttcggg taaatccacg ctggcaaaaa tgctggcggg aatgatagag
    19321 cctaccagcg gcgagttatt gattgacgat catccactgc attttggcga ttattccttc
    19381 cgcagtcagc gtattcgcat gatttttcag gatccttcga cctcgttgaa tccccgtcaa
    19441 cgtatctcgc aaattctgga ttttccactg cgcctgaaca ccgacctgga accggaacag
    19501 cgtcgcaaac agattattga aacgatgcgt atggttgggc tgctaccgga tcacgtcagt
    19561 tactatccgc atatgttggc acccggacaa aaacagcgtc tgggtctggc gcgcgcgctg
    19621 atattgcgcc caaaagtcat tattgccgat gaagccctcg cttcactgga tatgtcgatg
    19681 cgttcgcagt tgattaatct gatgctggag ttacaggaaa aacagggcat ttcgtatatt
    19741 tatgttaccc agcatatcgg aatgatgaag cacattagcg accaggtgct ggtgatgcat
    19801 cagggcgagg ttgtcgagcg aggcagcacc gcggatgtgc tggcatcacc gctgcatgaa
    19861 ctcaccaaac ggctgattgc cgggcacttt ggcgaagcat tgacagcgga tgcgtggcgt
    19921 aaagatcgct aattttctga atcctccttc cagattattc acctcattct gacaagagat
    19981 taaatcttgt cagaatgagg tgatggataa aataaaatca aatgcgcgtg atttaagaag
    20041 caatctcact ttgcaggaac gaaagctctg gcgatatctt cgcagccgac gttttggtga
    20101 tttcaaattt cgccgtcaac atccggtggg gagctacatt ctcgattttg cttgctgctc
    20161 ggcgcgtgta gtcgttgagc tggatggtgg gcagcatgat ttggcagttg cctatgattc
    20221 caggcgcact agctggcttg agtcgcaggg ctggaccgtg ctgcgtttct ggaataacga
    20281 gattgattgt aatgaggaga cggtgctgga gaatattctg caggaactga accgccggtc
    20341 accctctccc tgaaagagcg agggggccga ccgagccgaa tagctgttgt ggtgaaaata
    20401 tggtgacagt tctggagaat attcggcaag gtctgaaccg tcccagccat cgccctgaaa
    20461 gtgttagggg gctgtataag cctgtttgtt gctggggtga caatatttgc acaatacggt
    20521 cccctcgccc ctctggggag aggattaggg tgaggggaaa agcgcccccc ctgccgcagc
    20581 ctgctccggt cggacctggc aactatagct actcacagcc aggttgatta taataaccgt
    20641 ttatctgttc gtactgttta ctaaaacgac gaatcgcctg attttcaggc acaacaagca
    20701 tcaacaataa ggattaaagc tatgggtttt ctttccggta agcgcattct ggtaaccggt
    20761 gttgccagca aactatccat cgcctacggt atcgctcagg cgatgcaccg cgaaggagct
    20821 gaactggcat tcacctacca gaacgacaaa ctgaaaggcc gcgtagaaga atttgccgct
    20881 caattgggtt ctgacatcgt tctgcagtgc gatgttgcag aagatgccag catcgacacc
    20941 atgttcgctg aactggggaa agtttggccg aaatttgacg gtttcgtaca ctctattggt
    21001 tttgcacctg gcgatcagct ggatggtgac tatgttaacg ccgttacccg tgaaggcttc
    21061 aaaattgccc acgacatcag ctcctacagc ttcgttgcaa tggcaaaagc ttgccgctcc
    21121 atgctgaatc cgggttctgc cctgctgacc ctttcctacc ttggcgctga gcgcgctatc
    21181 ccgaactaca acgttatggg tctggcaaaa gcgtctctgg aagcgaacgt gcgctatatg
    21241 gcgaacgcga tgggtccgga aggtgtgcgt gttaacgcca tctctgctgg tccgatccgt
    21301 actctggcgg cttccggtat caaagacttc cgcaaaatgc tggctcattg cgaagccgtt
    21361 accccgattc gccgtaccgt tactattgaa gatgtgggta actctgcggc attcctgtgc
    21421 tccgatctct ctgccggtat ctccggtgaa gtagtccacg ttgacggcgg tttcagcatc
    21481 gctgcaatga acgaactcga acttaaataa tcgttctgtt agtaaagatg ggcggcgttc
    21541 tgccgcccgt tatctctgtt atacctttct gatatttgtt atcgccgatc cgtctttctc
    21601 cccttcccgc cttgcgtcag gataacgatt tcctttacga ccaaggagcg cccatggaac
    21661 aacgccacat caccggcaaa agccactggt atcatgaaac gcaatccagt actgcggagt
    21721 atgacgttct gcctctggtc ccggaagccg caaaggtcag cgatcccttt ctgctcgacg
    21781 tgatccttga tgaagaaacg ctggctccct tcctttcatg gctggtccct gcgcgcgttc
    21841 ttgcagtgga attgttccct gaccagctta ccgtgacccg ttcacagacc ttcaccgctt
    21901 atgaacgctt gtctacggcc ctgacggttg ctcaggtttg cggcgtccag cggttatgta
    21961 actactattc ggcgcgactt acgccgctcc ccgggcctga ttcctccagg gaaagcaatc
    22021 ataggttggc acaaatcacg caatatgccc gccaactggc tagctcgcct tctattatcg
    22081 acaaccgatc gcgccagcat ctgaatgacg tcggtcttac tgcctgggac tgtgtgatca
    22141 ttaaccaaat cattggtttt attggctttc aggcgcggac cattgcgaca tttcaggctt
    22201 atctcgggca cccggtacgc tggttacccg gtctggaaat acaaaactac gccgacgcgt
    22261 cactgtttgc tgatgaatca atacgctggc gaagcagcta tgaggtggaa aaactacctg
    22321 aagagtacac aaaaagttca actgcagaac tttgccaact ggccgaaaca ctctctctcc
    22381 accctatttc actttccctt ctcgaaaggt tgttaaacag tacacgggtt aatacacagc
    22441 cggataataa gcttgcggcg ttgttatgcg cgcggataaa tggcagtcct gcttgttttg
    22501 ccgcctgtat ggattcatca aatgaatata aaaaaatcag cgcccttgtg cgcaagggcg
    22561 aaaatgaaat taaccaatgg gctgaccgtc attctgttga gcacgctacc gttcaggcga
    22621 tacaatggct gacccgagca cccgatcgct ttagcgccgc ccagttcagc cctttactcg
    22681 aacacgaaaa atcatcaacg cagattatta atctgctggt atggagcggg ctgtgtggct
    22741 ggataaatcg cttaaaaatc gcgttgggtg agacatatta accttgccgc gtcagacaga
    22801 ttcgcgtaaa actgtcagcc gctctaatgg ccaccaaaat agacaattat gtttcaggac
    22861 aacccgctgc tagcgcagct taaacagcaa ctgcattccc agacgccacg cgctgaaggg
    22921 gtggtaaaag ccacagaaaa aggctttggc ttcctggaag tcgacgcgca aaaaagttat
    22981 ttcattccgc cgccgcagat gaaaaaagtc atgcatggcg accgaattat cgcggtgatc
    23041 cacagtgaaa aagaacgtga atccgcagag ccagaagaac tggttgaacc gttcctgact
    23101 cgtttcgtgg gtaaagttca gggcaaaaat gaccgtctgg ccatcgttcc tgatcatcca
    23161 ctcttaaaag acgccattcc ttgccgcgca gcccgtggcc tgaaccacga gtttaaagaa
    23221 ggcgactggg cggttgccga aatgcgccgt catccgctga aaggcgatcg ttctttctat
    23281 gcagaactga cacaatacat cacttttggt gacgaccact ttgtaccgtg gtgggttacc
    23341 cttgcgcgcc ataatctgga aaaagaagca ccagacggcg tcgctaccga aatgctcgat
    23401 gaaggtctgg ttcgtgaaga tctgaccgcg ctggattttg tcaccatcga cagtgccagc
    23461 acagaagata tggatgacgc ccttttcgct aaggcgttgc cggatgacaa acttcagctg
    23521 attgtggcga ttgccgatcc aaccgcgtgg attgctgaag gcagcaagct ggacaaagcc
    23581 gcgaaaattc gcgcattcac caactatctg cctggcttca acatccctat gctgcctcgc
    23641 gagctttctg acgatctctg ctcactgcgc gccaatgaag tccgcccggt actggcatgc
    23701 cgcatgacgc tctccgctga tggcaccatt gaagataata tcgaattctt tgccgccacc
    23761 atcgaatcca aagcaaagct ggtgtatgac caggtttctg actggctgga aaataccggt
    23821 gactggcagc ctgaaagtga agcaattgcc gaacaagtcc gtttgctagc gcaaatttgc
    23881 caacgccgcg gcgagtggcg tcataaccac gcactggtgt ttaaagatcg cccggattac
    23941 cgctttattc tcggtgaaaa aggtgaagtg ctggatatcg tcgccgagcc tcgtcgcatt
    24001 gccaaccgta tcgtcgaaga agcgatgatt gccgctaaca tttgtgcggc ccgcgtactg
    24061 cgcgataagc tcggttttgg catctataac gtgcatatgg gctttgatcc ggcgaatgcc
    24121 gacgcgctgg cagcgttgct gaaaacgcac ggtctgcatg tcgatgccga agaagtgctc
    24181 acgctggacg gtttctgcaa actgcgtcgt gaactggatg cgcaaccaac tggtttcctc
    24241 gacagccgca ttcgtcgctt ccagtcattt gctgaaatta gcactgaacc cggtcctcac
    24301 tttggcctcg gtctggaagc atacgccacc tggacatcgc cgatccgtaa atatggcgac
    24361 atgatcaacc accgtctgct gaaagcggtt atcaaaggcg aaactgcgac gcgtccacag
    24421 gatgagatca ctgtccaaat ggccgagcgt cgccgtctca atcggatggc agaacgtgat
    24481 gttggtgact ggttatacgc acgcttcctg aaagacaaag ccgggaccga cacccgtttc
    24541 gcggcggaaa ttgtcgatat cagccgtggc ggcatgcgtg ttcgtttggt tgataacggc
    24601 gctatcgcct ttattcctgc acctttctta cacgctgtgc gcgatgaaat ggtttgcagc
    24661 caggaaaacg gcaccgtaca aattaaaggt gaaacggttt ataaagtaac tgacgttatt
    24721 gacgtcacca ttgccgaagt ccgcatggaa acccgcagca ttattgcgcg cccggtcgcg
    24781 taatctcctt tcacggccca ttccttatga atgggccgtt tatttccccg ctctaccttc
    24841 atcatatccc ggcagttttt taactgactt tcatttgaaa actggcgttt ttccaaaact
    24901 ggattacaaa taaatatata aaaaaacaat gcgtttagcc gccgttaatt tcatgtaagt
    24961 catgttgttc cttttattta accgcggact ccgctgttaa ccggaggata tgcatcatga
    25021 aaaccgttag ggagtccaca acgttgtaca actttctcgg atcgcacaat tcatactggc
    25081 ggttgacgga aagcagcgat gttttgcgct tttctaccac cgaaaccaca gaacctgaac
    25141 gtatgttgca gttatctgcc gaacaggctg ctcgaatcag ggaaatgacg gtcatcacct
    25201 ccagcctgat gatgagtctg accgtcgatg aaagcgatct ttctgtgcat ctggtaggac
    25261 gaaaaatcaa taaacgggaa tgggctggca acgcgtctgc atggcatgac acaccggcgg
    25321 ttgctcgtga tttatcacac gggctttcct ttgctgagca ggtagtttct gaagcacatt
    25381 ccgcaatagt cattctcgac agccggggga atattcaacg cttcaatcgc ttatgtgaag
    25441 attacacagg gttgaaagaa cacgacgtca ttgggcaaag cgtgtttaaa ctgtttatga
    25501 gccgtcgtga agctgcggca tccaggcgca ataaccgtgt attttttcga agcggcaatg
    25561 catatgaagt cgaactgtgg ataccaacac gtaaaggcca gcggctgttt ctgtttcgca
    25621 ataaatttgt ccacagcggc agtggcaaaa acgagatttt tttaatctgt tccggtaccg
    25681 acattaccga agagcgccgc gctcaggagc gactgcgtat tctggcaaat accgacagta
    25741 tcaccggact gccgaatcgt aacgcaatgc aggagttaat cgatcacgct attaatcatg
    25801 cagataacaa taaagttggg gttgtgtatc ttgatttgga taattttaaa aaggtcaacg
    25861 acgcctatgg gcatttgttt ggtgaccagt tattacgcga cgtgtcattg gctattttaa
    25921 gctgtctcga acatgaccag gtgttggcgc gtccaggtgg cgatgagttt ctggtactgg
    25981 catccaatac ctcacaaagc gcgctggaag caatggcatc acgaattttg acccgcttac
    26041 gcctcccctt tcgtattggt ttaattgaag tttataccag ctgttcagta ggtattgcac
    26101 tctctcccga acatggttcg gacagcgcgg ctattattcg tcacgccgac acagcaatgt
    26161 acacagcgaa ggaaggcgga cgaggacaat tttgcgtttt taccccagaa atgaatcaac
    26221 gggtatttga atatctctgg ctggatacca acttgcgtaa agcactagaa aacgatcagt
    26281 tggttattca ctatcaaccg aaaatcacct ggcgtggcga agtgcgcagt ctggaagcac
    26341 tagtacgttg gcagtcacct gaacgtgggt tgattccacc gttggacttc atttcctacg
    26401 ccgaagagtc agggctaatt gtgcctttag gccgttgggt gattctcgat gtcgtacgcc
    26461 aggtggcaaa gtggcgggat aaaggcataa acctgcgagt ggcggtaaat atttctgcac
    26521 gtcagctcgc cgatcaaacc attttcaccg ccctgaaaca ggttctccag gaactcaatt
    26581 ttgaatactg ccctatagat gttgaactga cagagagttg tctgattgag aatgatgaac
    26641 tggcactgtc tgttattcaa caatttagcc aactaggtgc gcaagtacat ctggatgatt
    26701 ttggtaccgg ctactcttca ctttcgcaac tggcgcgctt tccgatcgat gccatcaaac
    26761 ttgaccaggt ttttgttcga gatattcaca aacaacctgt ctcgcagtca ctggtccggg
    26821 cgatcgtcgc tgtggcccag gcattgaatc ttcaggtgat cgccgaaggt gtagagagtg
    26881 caaaggaaga tgctttttta accaagaacg ggatcaatga gcggcaagga tttttgtttg
    26941 ccaaaccgat gcccgccgtc gccttcgaac gctggtataa acgctatctg aagcgcgcat
    27001 aagtagatga atatatatac ttagcgctat atcccgtcat tatatatcgt attcctggat
    27061 gataattagc cgttgctgtt aaacacttct cttttagcgg caatttttgc attattcatt
    27121 ttccactttt gctataagga tgtccttgat gtctgaattt gatgcccagc gcgttgccga
    27181 acgtattgat attgtgttag atatccttgt ggcaggcgat taccactctg cgatccataa
    27241 tctcgagata cttaaagcag aactcttgcg ccaggttgct gaatctacgc cagacatccc
    27301 gaaggcaccg tgggagattt aacaatcgtg ccttcaggtt ttcctatttg aggatgcccg
    27361 gcgcgtcagt atttttttat taagtattat aacgttataa gaattacagc gatgaattcc
    27421 cgacaacaaa ctattctaca gatggtcatt gaccagggtc aggttagcgt aaccgatctg
    27481 gcaaaagcca ctggagtttc tgaagttacc attcgccagg atctcaacac actcgaaaaa
    27541 ctgagttacc tccgccgtgc acatggcttt gctgtttcgc ttgatagtga tgacgtcgaa
    27601 acccgtatga tgagcaacta tacgctgaag cgtgaactcg ccgagtttgc cgcgtcgctg
    27661 gttcaaccgg gcgaaaccat ctttatcgaa aatggcagca gcaatgccct actcgcccgg
    27721 actctgggcg agcagaagaa aaatgtcact atcatcacgg tcagcagcta catcgcgcat
    27781 ttgctgaaag acgcgccttg tgaagttatt ttgctcggtg gcgtgtatca gaaaaaaagc
    27841 gaaagtatgg ttggcccttt gacacgccag tgcatccaac aggtgcattt cagcaaagca
    27901 tttattggta ttgatggctg gcaacctgaa actggattta ccggtcgcga tatgatgcgt
    27961 accgatgtgg tcaatgccgt gctggaaaaa gagtgcgaag cgatagtcct gactgacagc
    28021 tcgaaatttg gtgctgtaca ttcatactcc atcggtcccg ttgagcgatt caatcgcgtg
    28081 attaccgatt cgaaaatacg cgccagcgat ctgatgcatc ttgagcacag taaactcacc
    28141 gttcacgtcg ttgacattta accatttccc ttctctacgg atgatttgca gtttggcaaa
    28201 tcatccgctc taagatgatt cctggttgat aattaa
//
LOCUS       DAPHBJ010000040        28225 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.040, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000040 DAPHBJ010000000
VERSION     DAPHBJ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.040"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(322..1242)
                     /gene="malM"
                     /locus_tag="RHZ76_001583"
     CDS             complement(322..1242)
                     /gene="malM"
                     /locus_tag="RHZ76_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HDV2812239.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(1485..2825)
                     /gene="lamB"
                     /locus_tag="RHZ76_001584"
     CDS             complement(1485..2825)
                     /gene="lamB"
                     /locus_tag="RHZ76_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HDV2812240.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(2897..4012)
                     /gene="malK"
                     /locus_tag="RHZ76_001585"
     CDS             complement(2897..4012)
                     /gene="malK"
                     /locus_tag="RHZ76_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HDV2812241.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4377..5567
                     /gene="malE"
                     /locus_tag="RHZ76_001586"
     CDS             4377..5567
                     /gene="malE"
                     /locus_tag="RHZ76_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HDV2812242.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            5721..7265
                     /gene="malF"
                     /locus_tag="RHZ76_001587"
     CDS             5721..7265
                     /gene="malF"
                     /locus_tag="RHZ76_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HDV2812243.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            7280..8170
                     /gene="malG"
                     /locus_tag="RHZ76_001588"
     CDS             7280..8170
                     /gene="malG"
                     /locus_tag="RHZ76_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HDV2812244.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8542..10017
                     /gene="xylE"
                     /locus_tag="RHZ76_001589"
     CDS             8542..10017
                     /gene="xylE"
                     /locus_tag="RHZ76_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HDV2812245.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSSDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10061..10471)
                     /gene="psiE"
                     /locus_tag="RHZ76_001590"
     CDS             complement(10061..10471)
                     /gene="psiE"
                     /locus_tag="RHZ76_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HDV2812246.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(10597..10740)
                     /locus_tag="RHZ76_001591"
     CDS             complement(10597..10740)
                     /locus_tag="RHZ76_001591"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812247.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPTLVRFVLPDAPPGAAYP
                     AYG"
     gene            10741..11019
                     /locus_tag="RHZ76_001592"
     CDS             10741..11019
                     /locus_tag="RHZ76_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812248.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(11066..13162)
                     /locus_tag="RHZ76_001593"
     CDS             complement(11066..13162)
                     /locus_tag="RHZ76_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDV2812249.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNLLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13162..13899)
                     /locus_tag="RHZ76_001594"
     CDS             complement(13162..13899)
                     /locus_tag="RHZ76_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDV2812250.1"
                     /translation="MIKQTFVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(13896..14534)
                     /locus_tag="RHZ76_001595"
     CDS             complement(13896..14534)
                     /locus_tag="RHZ76_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HDV2812251.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIIDGATWTRTMGWTEYKQVRYATARSVFKWNGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSVGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(14649..14891)
                     /locus_tag="RHZ76_001596"
     CDS             complement(14649..14891)
                     /locus_tag="RHZ76_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812252.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(15403..17052)
                     /gene="pgi"
                     /locus_tag="RHZ76_001597"
     CDS             complement(15403..17052)
                     /gene="pgi"
                     /locus_tag="RHZ76_001597"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDV2812253.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            17577..18926
                     /gene="lysC"
                     /locus_tag="RHZ76_001598"
     CDS             17577..18926
                     /gene="lysC"
                     /locus_tag="RHZ76_001598"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HDV2812254.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDSRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            19179..19451
                     /locus_tag="RHZ76_001599"
     CDS             19179..19451
                     /locus_tag="RHZ76_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HDV2812255.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     DYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(19584..20456)
                     /gene="rluF"
                     /locus_tag="RHZ76_001600"
     CDS             complement(19584..20456)
                     /gene="rluF"
                     /locus_tag="RHZ76_001600"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HDV2812256.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKVKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            20668..21357
                     /gene="pepE"
                     /locus_tag="RHZ76_001601"
     CDS             20668..21357
                     /gene="pepE"
                     /locus_tag="RHZ76_001601"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HDV2812257.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPIAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(21448..23079)
                     /locus_tag="RHZ76_001602"
     CDS             complement(21448..23079)
                     /locus_tag="RHZ76_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDV2812258.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARENLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(23299..26982)
                     /gene="metH"
                     /locus_tag="RHZ76_001603"
     CDS             complement(23299..26982)
                     /gene="metH"
                     /locus_tag="RHZ76_001603"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDV2812259.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALSFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVTEVERWLAPNLGYDAD"
     gene            27182..28006
                     /gene="iclR"
                     /locus_tag="RHZ76_001604"
     CDS             27182..28006
                     /gene="iclR"
                     /locus_tag="RHZ76_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HDV2812260.1"
                     /translation="MVAPIPAKRGRKPAAATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         6578 a         7462 c         7253 g         6932 t
ORIGIN      
        1 tagtaaatca agatctacta tattatttcc ttgcaccaca tcatgtttca tcaaagagtt
       61 attgccgcca gaatttagga tatgacttat cataaaacct ctgcataaca gaaataaaat
      121 aacctggaaa agtagcctga atgtccagac actaccggcg aaaggaaaag aatctctgat
      181 aaggcattga gataatggat attcttagtg aagaagaagt gagttactat aaaaaatcgc
      241 tcatatccca ggaaggaagc ataaaaaaag gtgcgccagg agacgcacca gttgttgcaa
      301 aatcagcact gtggggcgta attacccctt gccttttaca ctgctgataa aggtggaacg
      361 ggcagatgtc gatcccaggc gttcagcttc atcaagcagt tttaacgcct tatcaacatc
      421 acctttcgcg acagcgtttt tgatcgcggt attaaaataa ctttccgtgt cgttgagcat
      481 cggctcgctt ttcttcaccg gtgccggagc gggtgcagcc acagctggtg ccgccgtgtt
      541 acctaccgta accggagctg gagcggaaga accaaataaa ggtcctacca acacgctgga
      601 gctggagttc gttttcactt tcagtttcag taagccatcg gtggtatgac gagcaaccgg
      661 atcggggata tccgggatcg agttaccgac gcccttggca taggctttag ccgggtcgag
      721 cagttgggtc gtctgctgga gatctttttc cgtggtaaag accagaacat aaagtttttg
      781 ctgccccaac gccggtgtca ggcgcataac gccttccagc cgatctgcac tcatcacgcc
      841 tggttcctgg taggtgaaat aactgctggg gaagaaggct gatggggtca tgttctgatc
      901 aagaatcagc acgttcggcg caaaaacgct ggtttgtttg ttcacttcgc tggtcagcgt
      961 cagggtcagt tcgccaatgt ttgccgggac gctgtacgca gcaaccggac cactgatgcc
     1021 gggaacgttc agttgttggc cgccggtcgc cagttgggtg gtctgggttt tagattgatc
     1081 gaccggtgtc caggtgagtt gttgcagcgc agcagatgga atggctggcg cgtcgctggt
     1141 gttttgcggt acgtagttaa catcggcaag gctgattcca ggcgcgcttg ccagtaaccc
     1201 tgctgataaa cagagggcga tgagactttt attcattttc attgttttca cctcaaaatc
     1261 tggagctcag cggtagccag gcaatagcgc gctaaacccg ataatcagag gggctttcgc
     1321 ccctttcaga taatgacaac ctgtttttat gccggatgcg gcgtaaacgc cttatccggc
     1381 ctacatttga cagccgttgt aggcctgata agacgcgcaa gcgtcgcatc aggcgttagt
     1441 tgccgaatgc ggcgtaaacg ccttatccgg cccaggtttt gctattacca ccagatttcc
     1501 atctgggcac cgaaggtcca ctcgtcgctg tcgccacgac cgaagctgcc gccgttgaaa
     1561 tcagcaggaa cggctttgcc gtagttcggg ttaaccttgc tatcgccgtt gtagtcataa
     1621 ccccatttct catcccactt ggcgtaggtt gcgaagacac gaatagccgg gcgtgaccag
     1681 atgctgtcgc cagcctgcca ttgttgtgcg agggtaattt tgtactgatt gttcttgtcg
     1741 ccggtgcgct gggattcgac gttgtcgtag ccgatttcca tcacggtgct catgattggc
     1801 gtccacttgt acatcgggcg aataccaacg gtccaccact tggtgccgtt gtcgttatcc
     1861 cagttgatat cctggtacat acccacgtac atcatgtccc agttgtcgcc catggagatc
     1921 gcaccgtggt cgaggatacg cagcatgtga ccgttgttgt tgatattgta ggcaaatttt
     1981 tcgttatcaa acgcgacgcc agaaccctgc gacagacctt taccctgcga ggtcatcgaa
     2041 tcagtagcgt actgaacaac aaacttgtta aagcccttca ggacactctg agtatgttca
     2101 gcagtgaata accagccgtc tttcgatgcg ccatcaacca gacgatagtt atcacgcagg
     2161 ttggcacgac cgtagtcgac acccagttct aatgtgccgc ccgggttgat ttccatctgc
     2221 gctaaacgca catcgaaaac gtcgttcgcg gtttcgttgg tatagtcata aatattgttg
     2281 ctggcaaaag aggaagaacc accagcttca gaggagcggg ttgctgccag agagagttta
     2341 ccgaagccaa catcgatgtt ttccagaccg gcaccaggac cagaaatatc ccagtagtag
     2401 aagtcgatca tatgaacgtc atgacgttgg tagaagcgct tacctgccca gatggtggag
     2461 cctggcagcc attcgatcag gtttttaccc tgcacgtttg cttcacggaa ggccggatcg
     2521 gtagcttccc agtcattctg ttgtgcgacg gaataggcca cgttagtgtc gaaatagaag
     2581 ctcttatcgc cctctttcca cacttcctga cccaatttta attcagcata agtttcacat
     2641 tcgttgccaa gacggtattt actttgagca ccggtagtct ggaaacactg ttgttcaccg
     2701 ccgctacctg tccaaccaat accggaacgt gcatagccgt ggaaatcaac agccattgcc
     2761 tgagcagaca ttacgcccgc tgcgacggca accgccagag gaagtttgcg cagagtaatc
     2821 atcattctat ctcctgagtc attgcttttc ttttttcaca tcacctgtga caggctttgt
     2881 gtgttttgtg gggtgcttaa acgcccggct ccttatgcag tcgacgacat gcagtgccat
     2941 cctcacggaa cagatggcaa cgctctggcg gcaggccgat agcgaatgtg gcaccttctt
     3001 ctaccaacac cacgtcgttc tggcggtaca ccaggttttg acgaatggaa gggatctgga
     3061 tatggatttg agtttcgttg ccgagttgct cgacgacctg aacttcaccc tcaaggatga
     3121 cgtcagcgat atcactcggc agtagatgtt ccgggcgaat acccagcgac atattggctc
     3181 caacctggac atcacggctt tcaactggca gccagacttg ctgacgattt ggcatcggca
     3241 gctccacctg cacttgatcg attgcggtgg cggtcacttt taccggcagg aagttcatct
     3301 ttggcgaacc gataaatccg gcgacaaaac ggtctgccgg atagtggtac agctccagcg
     3361 gtttcccaac ctgcgccacg cgaccggcgt ccagcaccac aattttgtcg gccagcgtca
     3421 tcgcttcgac ctgatcgtgg gtgacgtaaa tcattgtgcg gcccaggcgt ttatgcagac
     3481 gggagatttc gatacgcatt tgcacacgca gtgcagcatc gaggttggag agcggttcat
     3541 cgagcaaaaa tacgcttggc tcggccacca gcgtacggcc aatcgccaca cgctgacgct
     3601 gaccaccgga gagcgctttc ggtttgcgat ccagcaaatg cgccagttgt agcacttccg
     3661 ccacctggtt aacgcgttgg ttaatcacct ctttttttgc gccagccagt ttcaggccaa
     3721 atgacatgtt ttctgctact gacaggtggg gatagagcgc gtaagactga aacaccatac
     3781 caacgccgcg ttctgctggc ggagtgtcat tcatccgttt ctcaccgatg aacaggtcgc
     3841 cgctggtgat cgtctcaagc ccggcaatca tgcgcagtaa agtcgattta ccgcagccag
     3901 acggtccgac aaacaccacg aattcacctt catggatatc gagattgata tctttcgata
     3961 ccacgacctc gccccaggct ttcgttacat tttgcagctg tacgctcgcc atgcccttct
     4021 ccctttgtaa caacctgtca tcgacagcaa cattcatgat gggctgacta tgcgtcatca
     4081 ggagatggct taaatcctcc accccctggc ttttttatgg gggaggaggc gggaggatga
     4141 gaatacggct tctgtgaact aaaccgaggt catgtaagga atttcgtgat gttgcttgca
     4201 aaaatcgtgg cgattttatg tgcgcatctc cacattaccg ccaattctgt aacagagatc
     4261 acacaaagcg acggtggggc gtaggggcaa ggaggatgga aagaggttgc cgtataaaga
     4321 aactagagtc cgtttaggtg ttttcacgag cacttcacca acaaggacca tagattatga
     4381 aaataaaaac aggtgcacgc atcctcgcat tatccgcatt aacgacgatg atgttttccg
     4441 cctcggctct cgccaaaatc gaagaaggta aactggtaat ctggattaac ggcgataaag
     4501 gctataacgg tctcgctgaa gtcggtaaga aattcgagaa agataccgga attaaagtca
     4561 ccgttgagca tccggataaa ctggaagaga aattcccaca ggttgcggca actggcgatg
     4621 gccctgacat tatcttctgg gcacacgacc gctttggtgg ctacgctcaa tctggcctgt
     4681 tggctgaaat caccccggac aaagcgttcc aggacaagct gtatccgttt acctgggatg
     4741 ccgtacgtta caacggcaag ctgattgctt acccgatcgc tgttgaagcg ttatcgctga
     4801 tttataacaa agatctgctg ccgaacccgc caaaaacctg ggaagagatc ccggcgctgg
     4861 ataaagaact gaaagcgaaa ggtaagagcg cgctgatgtt caacctgcaa gaaccgtact
     4921 tcacctggcc gctgattgct gctgacgggg gttatgcgtt caagtatgaa aacggcaagt
     4981 acgacattaa agacgtgggc gtggataacg ctggcgcgaa agcgggtctg accttcctgg
     5041 ttgacctgat taaaaacaaa cacatgaatg cagacaccga ttactccatc gcagaagctg
     5101 cctttaataa aggcgaaaca gcgatgacca tcaacggccc gtgggcatgg tccaacatcg
     5161 acaccagcaa agtgaattat ggtgtaacgg tactgccgac cttcaagggt caaccatcca
     5221 aaccgttcgt tggcgtgctg agcgcaggta ttaacgccgc cagtccgaac aaagagctgg
     5281 cgaaagagtt cctcgaaaac tatctgctga ctgatgaagg tctggaagcg gttaataaag
     5341 acaaaccgct gggtgccgta gcgctgaagt cttacgagga agagttggcg aaagatccac
     5401 gtattgccgc caccatggaa aacgcccaga aaggtgaaat catgccgaac atcccgcaga
     5461 tgtccgcttt ctggtatgcc gtgcgtactg cggtgatcaa cgccgccagc ggtcgtcaga
     5521 ctgtcgatga agccctgaaa gacgcgcaga ctcgtatcac caagtaatgc tgtgaaatgc
     5581 cggatgcggc gtgaacgcct tgtccggcct acaaaaccga aacgtatgta ggcctgataa
     5641 gacgcgtcag cgtcgcatca ggcagttgtt gtcggataag gcgtgaaagc cttatccgtc
     5701 ctggaatgag gaagaacccc atggatgtca ttaaaaagaa acattggtgg caaagcgacg
     5761 cgctgaaatg gtcagtgcta ggtctgctcg gcctgctggt gggttacctt gttgttttaa
     5821 tgtacgcaca aggggaatac ctgttcgcca ttaccacgct gatattgagt tcagcggggc
     5881 tgtatatttt cgccaatcgt aaagcctacg cctggcgcta tgtttacccg ggaatggctg
     5941 gaatgggatt attcgtcctc ttccctctgg tctgcaccat cgccattgcc ttcaccaact
     6001 acagcagcac taaccagctg acttttgaac gtgcgcagga agtgttgtta gatcgctcct
     6061 ggcaagcagg caaaacctat aactttggtc tttacccggc gggcgatgag tggcaactgg
     6121 cgctcagcga cggcgaaacc ggcaaaaatt acctctccga cgcttttaaa tttggcggcg
     6181 agcaaaagct gcaactgaaa gaaacgaccg cccagcccga aggcgaacgc gcgaatctgc
     6241 gcgtgattac ccagaatcgt caggcgctga gtgacattac cgccattctg ccggatggca
     6301 acaaagtgat gatgagctcc ctgcgccagt tttctggcac gcagccgctc tacacactcg
     6361 acggtgacgg cacgttgacg aataatcaga gcggcgtgaa atatcgtccg aataaccaaa
     6421 ttggctttta ccagtccatt accgccgacg gcaactgggg tgatgaaaag ctaagccccg
     6481 gttacaccgt gaccaccggc tggaaaaact ttacccgcgt ctttaccgac gaaggcattc
     6541 agaaaccgtt cctcgccatt ttcgtctgga ccgtggtgtt ctcgctgatc actgtctttt
     6601 taacggtggc ggtcggcatg gttctggcgt gtctggtgca gtgggaagcg ttgcgcggca
     6661 aagcggtcta tcgcgtcctg ctgattctgc cctacgcggt gccatcgttc atttcaatct
     6721 tgattttcaa agggttgttt aaccagagct tcggtgaaat caacatgatg ttgagcgcgc
     6781 tgtttggcgt gaagcccgcc tggttcagcg atccgaccac cgcccgcacg atgctaatta
     6841 tcgtcaatac ctggctgggt tatccgtaca tgatgatcct ctgcatgggc ttgctgaaag
     6901 cgattccgga cgatttgtat gaagcctcag caatggatgg cgcaggtccg ttccagaact
     6961 tctttaagat tacgctgccg ctgctgatta aaccgctgac gccgctgatg atcgccagct
     7021 tcgcctttaa ctttaacaac ttcgtgctga ttcaactgtt aaccaacggc ggcccggatc
     7081 gtcttggcac gaccacgcca gccggttata ccgacctgct tgttaactac acctaccgca
     7141 tcgcttttga aggcggcggg ggtcaggact tcggtctggc ggcagcaatt gccacgctga
     7201 tcttcctgct ggtgggtgcg ctggcgatag tgaacctgaa agccacgcga atgaagtttg
     7261 attaagggag ataacaaaaa tggcaatggt ccaaccgaaa tcgcaaaaag ctcgtttatt
     7321 tattactcac ctgctactgc tactttttat cgcagcgatt atgttcccgc tgctgatggt
     7381 cgtcgctatc tcgctgcgtc agggaaactt tgcgaccggc agcctgatcc cggagcaaat
     7441 ctcctgggat cactggaaac tggcgttagg ttttagcgtt gaacaggctg atggtcgcat
     7501 tacgccaccg ccattcccgg tactgctgtg gctgtggaac tcggtaaagg tcgccgggat
     7561 ttccgcgatt ggcattgtgg cgctctccac cacctgcgcc tacgctttcg cccgtatgcg
     7621 ctttccaggc aaagcgacgc tgctgaaagg aatgctgatt ttccagatgt tcccggcagt
     7681 actttcactg gtcgcgttgt atgcgttgtt tgatcgtctg ggtgagtaca ttccattcat
     7741 tggcctgaat actcacggcg gcgtaatttt cgcgtatctg ggtgggattg cgctgcatgt
     7801 ctggaccatc aaaggctatt tcgaaaccat cgacagttcg ctggaagaag ctgctgcgct
     7861 ggatggtgcg acaccgtggc aggccttccg ccttgtcctg ttgccgctgt cagtaccgat
     7921 tctggcggtg gtattcatcc tgtcgtttat cgctgccatt actgaagttc cggtcgcgtc
     7981 gctgttactg cgtgacgtaa acagctacac cctggccgtg gggatgcagc aatacctcaa
     8041 cccgcaaaac tacctgtggg gtgactttgc cgccgctgcc gtgatgtctg cattaccgat
     8101 caccatcgtc ttcttgctgg ctcaacgctg gctggtcaac ggcctgacgg caggtggtgt
     8161 gaaaggttaa agatgttgtt ctgccaatgt tatgccgctg caccctcaac ttacgttatc
     8221 ccaacttgtg actgttattc ggcgctccac ggagcgcctt tttttctttc gtctgcaatc
     8281 tgaatcgttc gccggttaat atttccatca tagagcttat tatttttacg ttatttgttt
     8341 tcccacttac gataattctc tttcgtgctc tgagtcacgg caatagtatt gtttttatca
     8401 attttggata attatcacaa ttaagatcac agaaaagaca ttacgtaaac gcattgtaaa
     8461 aaatgataat tgccttaact gcctgacaat tccaacatca atgcactgat aaaagatcag
     8521 aatggtctaa ggcaggtctg aatgaatacc cagtataatt ccagttatat attttcgatt
     8581 accttagtcg ctacattagg tggtttatta tttggctacg acaccgccgt tatttccggt
     8641 actgttgagt cactcaatac cgtctttgtt gctccacaaa acttaagtga atccgctgcc
     8701 aactccctgt tagggttttg cgtggccagc gctctgattg gttgcatcat cggcggtgcc
     8761 ctcggtggtt attgcagtaa ccgcttcggt cgtcgtgatt cacttaagat tgctgctgtc
     8821 ctgtttttta tttctggtgt aggttctgcc tggccagaac ttggttttac ctctataaac
     8881 ccggacaaca cagtgcctgt ttatctggca ggttatgtcc cggaatttgt tatttatcgc
     8941 attattggcg gtattggcgt tggtttagcc tcaatgctct cgccaatgta tattgcggaa
     9001 ctggctccag ctcatattcg cgggaaactg gtctctttta accagtttgc gattattttc
     9061 gggcaacttt tagtttactg cgtaaactat tttattgccc gttccagtga tgccagctgg
     9121 ctgaatactg acggctggcg ttatatgttt gcctcggaat gtatccctgc actgctgttc
     9181 ttaatgctgc tgtataccgt gccagaaagt cctcgctggc tgatgtcgcg cggcaagcaa
     9241 gaacaggcgg aaggtatcct gcgcaaaatt atgggcaaca cgcttgcaac tcaggcagta
     9301 caggaaatta aacactccct ggatcatggc cgcaaaaccg gtggtcgtct gctgatgttt
     9361 ggcgtgggcg tgattgtaat cggcgtaatg ctctccatct tccagcaatt tgtcggcatc
     9421 aatgtggtgc tgtactacgc gccggaagtg ttcaaaacgc tgggggccag cacggatatc
     9481 gcgctgttgc agaccattat tgtcggagtt atcaacctca ccttcaccgt tctggcaatt
     9541 atgacggtgg ataaatttgg tcgtaagcca ctgcaaatta tcggcgcact cggaatggca
     9601 atcggtatgt ttagcctcgg taccgcgttt tacactcagg caccgggtat tgtggcgcta
     9661 ctgtcgatgc tgttctatgt tgccgccttt gccatgtcct ggggtccggt atgctgggta
     9721 ctgctgtcgg aaatcttccc gaatgctatt cgtggtaaag cgctggcaat cgcggtggcg
     9781 gcccagtggc tggcgaacta cttcgtctcc tggaccttcc cgatgatgga caaaaactcc
     9841 tggctggtgg cccatttcca caacggtttc tcctactgga tttacggttg tatgggcgtt
     9901 ctggcagcac tgtttatgtg gaaatttgtc ccggaaacca aaggtaaaac ccttgaggag
     9961 ctggaagcgc tctgggaacc ggaaacgaag aaaacacaac aaactgctac gctgtaatct
    10021 tcctgtccag cacgccgcgc catttcggcg tgctgacttt ttactcccgc ttcagccgtt
    10081 tcgaattaca cagccacagg gtgatcacca gtaacaggat cgcagccgag tagatcaaca
    10141 catccagtgg cgatttatga tcgacgatga tcaagcgcac aatggcggtg atcccaatat
    10201 agacaaagta acgtagcggg aagtgaaaac cggactgaaa gtacttcaca atcagcgcga
    10261 taaattcgaa atagagaaag taaaccacca gtccttctac cagctcatat ttgctggttt
    10321 gttctggcgc gaacagcaca tcagccagat gcaccgtttc tttgccgagg aagacaacca
    10381 aaatcaggcc gaggcacagc aggccaagat tgagtacggt ctgcaaaatg gtggagataa
    10441 actccacgcg cggacgagag agtgacgtca tagcagtacc tccttcacag tggcgagctt
    10501 cctgtataac gcaaaaatgt gacggagatc tatatttttt gtgtatgttt tgttcaacct
    10561 ggatttggag cgcgcctcca ccaaagccgg atatacctac ccgtaggccg gataagccgc
    10621 gcctggcggc gcatccggca agacaaaccg caccaaagtc ggtaattcaa aaatcaccca
    10681 ttcaggtgat tcccagccac cagcaatgcg cgaaaaatcg acaatgttcc tctggaacaa
    10741 atgaaacgta acctgataaa ggtggtaaaa atgaagcctt attttgctgc tttgatgtta
    10801 tcagtctctg ttcttcctgc ttatgctggt ccgctcggta ctgctgataa agccgaccta
    10861 ccgcaaagca atgtctccag cccaatgatg gcccagtcgc tcaggcaacc agacctgcaa
    10921 ccgatatcga cggacaggaa gacagaatgt tttcgccttt acacaccaga tcgcaaaccg
    10981 ggagtgaact gcgttcctga tggctcgaca ggccattaag aatgaaggtg ccagtgagtg
    11041 cgaaaactgg cacctttccc atgacttagc ggaaattgac gctacggtcg ctggtcatgt
    11101 catacaactg gaacttacgc ccaagttgct gaccaccgtc acgcgtcagc ggcgtccagc
    11161 caatcgccgc acggctgcgt gtcgggccag acgagaagag atccaacggt accgagacat
    11221 acacgccttt ggtgaagtcc ccttcgccgt actcctcttt cgaaacatta gtgatcgtgg
    11281 catagccacc caccaccacg ccgctatcaa agcgtttggc gatctccagc gtgccgcctt
    11341 tatcccctgc cagatactgc ccgacgctgg ctttaactaa cacatcctga gcgaaagatg
    11401 gcgtccagta ggcggtcaga tgtccggttt tcacgctgta gtcggtgaat ttcatcatat
    11461 cttttgcact acgccagtcg cgctgtttaa cgtagttggc atccagacca aacgcccagt
    11521 tgctgtccag cgggcgatac aacacttctg cccccgcacc gccaaacatg gtttcgagat
    11581 aaccaccgta gacctgaccg tagaagccgt tgcccagatg ctggaagtag ttggcttgca
    11641 ggttattcac atagacatcg ttctgcacat actcgcgcac atgggtacgc acgcgcggta
    11701 agtgcgagtc ctgcggagga ttagtgtagt taaacttgtc gtagttgttg gcaagatttg
    11761 caaacaggct gccggtggtc agcagatggt ccgtcagcca caaatctgcc gttcccatca
    11821 cgcccagctg atacatgtaa aagttttccg ggccaccgac cgactggttc agcaccggat
    11881 cgatatggaa atcaaagcgt gatttgtcga tataccagcc ctgctcggtg gactgcggaa
    11941 ccactggctc gacgcgtttt tgcgccagcg tcgtttcgtg gcccaacggc tctccggcga
    12001 gatgattttt caggctggcg acatcggttt ccgtcgtcac ctgcggcatg ttaaggcgat
    12061 tttccgtaat gcggatcgtt ttgatcccat ccggcagatc gttcatcacg atccgattcg
    12121 cacggatgat cccttcgcgc gaatcacgat atttcacctg ctcgccggta acatacagcg
    12181 tatcgccttt cgcctggatc tgtggatcgg caagtccagc attgtatttc aacagcgtta
    12241 actgattcgc caccaccgaa tgctgcaaaa tggcatcctg cggctgcggt tgatattgcg
    12301 ggcgggcgtt atcgttgtaa gacgggcgca gatcgttaaa gttggtgcgc aacgtaacgc
    12361 caaacataaa ggtgttgcca cgttcatagc taaggttaac gtcggcccaa tcggtaacgc
    12421 gataaatcgc accgacgtta aacttacttt tttgctccag cttcccggca aaatcctgct
    12481 gataattatt gccttcatac tccagtttca ggcgcagcgg ttgccagggc gtctggtatt
    12541 ccacgccgcc aaacagtgag gcaggaccgt ggaacatctg gctaccgtcg atagatcccg
    12601 cctgtttgta gctgttatcg cgatagcaat atttatcact ggctgaacag agcagatttt
    12661 tcacattacc gctggtgccc aaatatcccc agcccaggcc gagcgtaaaa tcgaacggcc
    12721 cccaggcttt gctggcaaca agatattccg catcaaacag ccccgtaccg ccgatatccc
    12781 gcgcaccaac cgccacttgc ggcagccagt aactctcttc ccacaaacgc agtttgagat
    12841 cgaaggcttt atctttatac gtttgatcgc cagagaacgc ttcgacgctg ctgtactgcc
    12901 gggtgcgcac gtcggtgtag cgcagcgttg tttccagcca cgggaagagt tgcactgaag
    12961 ctgagtaata acggtactga tcgttatcgc gatagttcag gctcaactcc ccttcccgcg
    13021 ccatgcgtgc ggtgggcgtt tgtaataatc ctacgccacc gaaatccgac tgcgacgggc
    13081 caatgggcgc aggatatgtt tcgccgtagc aagccatgct aatgcccagc gccagtaagc
    13141 taagcagatg tctttttttc attattgggg tatccgctgc gtcagagtct gaagaatgtc
    13201 ggcgttaagg gcatcaggcg tctcactcca gacggagtcc gcgaggccaa cataaataat
    13261 gctgccgggc atcggctcta cgtgacgctt gttccagtaa gccaccggcg ctttttgcgt
    13321 gcgtccgtcc gggtaaacca cccacgcgta gctgcgatcc gcaccgctaa gcaggttctg
    13381 cccggagaga tagctcgcca cgtcgcgacc tggagtgaat ggctgcttgc caggacggct
    13441 gataagcccg aacagcgtga ccgtggacgg tggtggcccg acccacagcg tgtagttgcc
    13501 ctgcaacggt gggttaccgc gttcggcaac gcggacgata tcaggatcaa gattgatttt
    13561 ttgcctgccc gtcactttca acgcctgaat ttgctggcgt aaggcgttaa tcgcggcggc
    13621 atcgtcggcg ctggaatctg cagccagttc tgccagccgt gtcagcaacg cctgctgctg
    13681 ccgcaatgct gccgccgttg ccagctcttc gctaatcacc gcaccgggcc accagctgtt
    13741 tgccagccgc ggttggccta ccagatcgat taaatgctct gcgcccgtta gcgttttggc
    13801 ctcactgctg ccattgctga acaccttgac ggttcctgcc gcgaagaccg atgacgctcc
    13861 cacactcaaa agcaacgcga caaaagtttg tttaatcatt gttttgccgc cttgatgaga
    13921 gtggttttca ccgggaaata atccgcgccg agatactgtt ccgactggcg aatttgccct
    13981 acgctgtcga tccaatagcg gttatgccag cgcgcctggt cggtggagac ttcttcgtcc
    14041 agcacgcgaa ccggggtttc atcgctgccg actttgacgg tatcggtacc attccatttg
    14101 aagactgatc gtgcggtggc gtagcgtacc tgcttatact cggtccagcc catcgtgcgc
    14161 gtccaggttg caccatcaat gatttgtgcg ggtttaatca gcgggtcggc ggcaaggtta
    14221 ttcacttcaa tcaggttgtc gccgccaagc aaagtcttca ccagacgacc atgttgtgtg
    14281 acgagagtgg cttgatcctg agtgacccat ttttgttgtc cgtcttcagc gaaggccagc
    14341 accacaaaca attgcggccc gccattaagc tgcatgtact ggctggcgta gggcatattt
    14401 tgaatatcat cgtccgtcag ctgtacgcct ggcgtgccga acagactgtc ccacagtgaa
    14461 ttgcccagct ctttagtcgt ggctgaacag gcctgtaata acaggcagat aagaatgagt
    14521 gcaggtcgct tcacgactct tctccagagg ggcagaataa ccacaccgga gtgtggttat
    14581 ggttaatccc cttcatattt caagccacag atgcgatgac tcgaattatt ttggggatac
    14641 atactttatt actgggtact ggtggttgtg gttgtggtgg tcccggtatt agaaccatca
    14701 ccgccaccgg ttgccgcgag cgcgacaccc accgccgagc ttacggtgct gacgctggtc
    14761 gcggaggaac tccccgccga aaccgacgtt gctgccgacc ctgccgcttc gcccacctgt
    14821 acaggtgcag cccacgcaga agtcgccgca agcgcagata tggcaaaaat gccatacaga
    14881 acttttttca taacaatttc ccttcattga atgaatggag atttacccaa aaacatttcg
    14941 ggcgggatcg agtatacaca actaaagcat gcgaattacg gcgcggggaa ttagcgatgg
    15001 tgttttagga taattggaat gaatatcaaa tataagtttc aacttatatt taaataacaa
    15061 taaaaatcat aaacttaaaa taaaaataaa agtaattaat ctgcgtattt tccgaaataa
    15121 acaacgatta aaaatctatt ttaggattaa cctcacggta tgatttccgt taaatcacag
    15181 acaaggggat ttatctgata aaaaaatgcc gacataacgt cggcattgtt attaaggtta
    15241 tttaatcaat cgtgctatgt attaattgcc gaatacgatg tacatatcgg catcgacctg
    15301 taggcctgat aagacgcgac cgcgtcgcat caggcatcgg ttgccggatg cggcgtgaac
    15361 gccttatccg gcctacatag cggcatcagg catgaacgat gattaaccgc gccacgcttt
    15421 atagcggtta atcagaccat tggtcgagct atcgtggctg ctgatttctt tatcatcttt
    15481 cagctctggc agaatacggt ttgccagttg tttacccagc tccacgcccc actggtcgaa
    15541 ggtgaagatg ttcaggatca cgccctgagt aaagattttg tgctcataca gcgcaatcaa
    15601 cgcccccagg ctgaacggag tgatttcgcg cagcaggatg gagttggtcg ggcggttacc
    15661 ttcgaacact ttgaacggca ccacgtagtc aagcgttgcc ggatctttac cctgatcgcg
    15721 atattcctgc tcaaccactt cacgggattt accaaacgcc agcgcttcgg tctgggcgaa
    15781 gaagttagac agcagtttct ggtggtgatc agagagcggg ttatgggtga tagccggggc
    15841 gatgaaatcg cacggtacca ttttggttcc ctggtggatc agctggtaga acgcgtgctg
    15901 accgttagtg cctggttcac cccagataat cgggccagtc tggtaatcca caacgttacc
    15961 gttacggtca acatacttac cgttggactc catattgccc tgctggaagt acgccgcgaa
    16021 acggtgcata tactggtcat acggcagaat cgcttcagtt tccgcaccaa agaaattgtt
    16081 gtaccagatg ccaatcagcg ccagcagtac aggcaggttt ttctcggcag gcgtggtgga
    16141 gaaatgcttg tccatcgcgt gtgcgccaga aagcagttca acgaagttat caaagccgat
    16201 ggagagaaca atcgacagac caatcgctga ccacaaagag taacggccgc caacccagtc
    16261 ccagaactcg aacatgttgg cagtatcaat accaaactcg ccaacggctt tggcattggt
    16321 ggagagcgca gcgaagtgtt tcgcaacgtg tttttcatca cctgccgctt tcaggaacca
    16381 gtcacgcgcg ctatgggcgt tggtcatggt ttcctgagtg gtgaaggttt tagatgctac
    16441 caggaacagc gtggtttccg ggtttacttt tttcagcact tcagcgatgt gagtcccatc
    16501 gacgttagaa acaaagtgca tgttcaggtg gtttttgtac ggacgcagtg cttcggtcac
    16561 catgtatggg ccgaggtcag aaccgccgat cccgatgttc actacgtcag tgattgcttt
    16621 gccggtataa cctttccact caccggaaat aatcgcttct gagaaggttt tcatcttctc
    16681 cagcaccgcg ttgacttccg gcattacgtc tttgccatca accaaaatcg gggtattgct
    16741 acggttacgc agcgctacgt gcagcacggc gcggttttca gtgcggttga tcttctcgcc
    16801 agagaacatc gacttaatcg cgcccgccag atcgcactct ttcgccagat cctgtaattt
    16861 cgccagcgtc tcttcggtga tgcggttttt ggagtaatcc accagcatct gatcgtcgaa
    16921 tgttgcggag aacttagaaa aacgatcgcc gtctttagca aaaagatcgg cgatcgtaac
    16981 gtctttcatt tcatcgaagt gtttctgtag tgcctgccag gcagcggtct gcgttggatt
    17041 gatgtttttc attagcaata ctcttctgat tttgagaatt gtgactttgg aagattgtag
    17101 cgccagtcac agaaaaatgt gatggtttta gtgccgttag cgtaatgttg agtgtaaacc
    17161 cttagcgcag tgaagcattt attagctgaa ctactgaccg ccaggagtgg atgaaaaatc
    17221 cgcatgaccc cgtcgttgac aaccgccccg ctcacccttt atttataaat gtactacctg
    17281 cgctagcgca ggccagaaga ggcgcgttgc ccaagtaacg gtgttggagg agccagtcct
    17341 gtgataacac ctgagggggt gcatcgccga ggtgattgaa cggctggcca cgttcatcat
    17401 cggctacagg ggctgaatcc cctgggttgt caccagaagc gttcgcagtc gggcgtttcg
    17461 caagtggtgg agcacttctg ggtgaaaata gtagcgaagt atcgctctgc gcccacccgt
    17521 cttccgctct tcccttgtgc caaggctgaa aatggatccc ctgacacgag gtagttatgt
    17581 ctgaaattgt tgtctccaaa tttggcggta ccagcgtagc tgattttgac gccatgaacc
    17641 gcagcgctga tattgtgctt tctgatgcca acgtgcgttt agtagtcctc tcggcttctg
    17701 ctggtatcac taatctgctg gtcgctttag cggaagggct ggaacctggc gagcgattcg
    17761 aaaaactcga cgctattcgc aacatccagt ttgccattct ggaacgtctg cgttacccga
    17821 acgttatccg tgaagagatt gaacgtctgc tggagaacat tactgttctg gcagaagcgg
    17881 cggcgctggc aacgtctccg gcgctgacag atgagctggt cagccacggc gagctgatgt
    17941 cgaccctgct gtttgttgag atcctgcgcg aacgcgatgt tcaggcacag tggtttgatg
    18001 tacgtaaagt gatgcgtacc aacgaccgat ttggtcgtgc agagccagat gtagccgcgc
    18061 tggcggaact ggccgcgctg cagctgctcc cacgcctcaa tgacggctta gtgatcaccc
    18121 agggatttat cggtagcgaa aataaaggtc gtacaacgac gcttggccgt ggaggcagcg
    18181 attatacggc agccttgctg gcggaggctt tacacgcatc tcgtgttgat atctggaccg
    18241 acgtcccggg catctacacc accgatccac gcgtggtttc cgcagcaaaa cgcattgatg
    18301 aaatcgcgtt tgccgaagcg gcagagatgg caacttttgg tgcaaaagta ctgcatccag
    18361 caacgttgct ccccgcagta cgcagcgata tcccggtctt tgtcggctcc agcaaagact
    18421 cacgcgcagg tggtacgctg gtgtgcaata aaactgaaaa tccgccgctg ttccgcgcgc
    18481 tggcgcttcg tcgcaatcag actctactca ctttgcacag cctgaatatg ctgcattctc
    18541 gcggtttcct cgcggaagtt ttcggcatcc tcgcgcggca taatatttcg gtagacttaa
    18601 tcaccacgtc agaagtgagc gtggcattaa cccttgatac caccggttca acctccactg
    18661 gcgatacgtt gctgacgcaa tctctgctga tggagctttc cgcactgtgt cgggtggagg
    18721 tggaagaagg tctggcgctg gtcgcgttga ttggcaatga cctgtcaaaa gcctgcggcg
    18781 ttggcaaaga ggtattcggc gtactggaac cgttcaacat tcgcatgatt tgttatggcg
    18841 catccagcca taacctgtgc ttcctggtgc ccggcgaaga tgccgagcag gtggtgcaaa
    18901 aactgcatag taatttgttt gagtaaatac tgtatggcct ggaagctata tttcgggccg
    18961 tattgatttt cttgtcacta tgctcatcaa taaacgagcc tgtactctgt taaccagcgt
    19021 ctttatcgga gaataattgc ctttaatttt tttatctgca tctctaatta attatcgaaa
    19081 gagataaata gttaagagaa ggcaaaatga atattatcag ttctgctcgc aaacgaattc
    19141 ccgtacactg aactctctgg ctcactgagg gcaccatcat ggcactcccc cgtattaccc
    19201 aaaaagagat gactgaacgc gagcagcgtg aacttaaaac actgctggat cgggcgcgga
    19261 ttgcgcatgg tcgcgtattg accaactccg aaaccaacag cattaagaaa gattacatcg
    19321 ataaattaat ggttgagcgt gaggctgaag cgaaaaaagc ccgccagttg aagaaaaagc
    19381 aggcttataa accggatcct gaagcgtcat ttagctggtc agcaaatacg tcgacgcgcg
    19441 gcaggcgcta atccacaatc aacgcctgag attttcactc gtcagtgtga acacaatttt
    19501 cccctcaccc taaccctttt cccgaagggg cgaggggact gtccgggcac ttttgggccg
    19561 ttttcatcag tctgaggcgt taatcagcgc cccttctttt tacgccccgg cgaggtaaaa
    19621 cgcttaccgt tggacgccgg gcgaccgcct ttttccgccg ttttttccat cttaacgact
    19681 ggacgtttga tgcccgctgt tttcggtttg gccttcactt taggttttgc ctcggaagag
    19741 gaattttcga tgagcttaaa gaggtcgatt aactcatcat cagttaaatc gcgccactca
    19801 cccagcggaa tgccgcttaa actgacgttc ataatgcgcg tacgttccag ctttttcact
    19861 tcgtaaccaa agtgctcgca catgcgacgg atctgacggt tcagcccctg caccagggta
    19921 atgcggaaga caaacggcgc ttcttttttg actttacact ttttagtcac tgtcccgagg
    19981 attggcaccc ccgcccccat accacgaata aattcatcgg taatcggttt atcgaccgta
    20041 accagatact ctttctcatg atcgttaccc gcacggagga tcttattcac cagatcgccg
    20101 tgattggtga ggaaaatcag cccctgggag tctttatcca ggcggccaat cgggaacacg
    20161 cgtttgctgt ggttaacgaa atcgacaatg ttatcgcgct cgccatcttc ggtggtgctg
    20221 acaataccga cgggcttgtt cagggcgata agtaccaaat cttcggcttc ccgaggctca
    20281 atcaactgac cgtttacttt cacaacgtcg ccgggtttca cctgatcgcc aatggtggct
    20341 cgcttgccat taaggaacac attgccttgc tcgatatagc gatccgcttc gcggcgtgag
    20401 caaattccgc tttcgctgat gtatttattt aaacggactg atgagtcggg cagcataaat
    20461 tctcctgtaa aaaacggaat ataccgtacc tttgggttga taaaaaatag tcatgagcga
    20521 actatttgtt atttccattg gcttgaattc gttacctgct caaatcactt tccagaagca
    20581 atgtgcgaca tcgtgagcca tgtcagtaag tggttaaatt cgcagtggta gcatgcctcc
    20641 cgtgatttct tactaaccgg agaagtaatg gaactgcttt tattgagtaa ctcgacgctg
    20701 ccgggtaaag cctggctgga acatgcgctg ccgctgattg ctgaacagtt gcagggtcgc
    20761 cgttcagcgg tatttattcc tttcgctggc gtaacgcaga cctgggatga ttacacagcg
    20821 aaaacggctg cggttctcgc tccgctgggt gtttctgtca ccggtattca tagcgttgtc
    20881 gatcccattg ccgctattga aaatgctgag atcgtgattg tcggcggcgg taatactttc
    20941 cagttgctga aacagtgccg cgaacgcggg ctgctggcac caattactga cgtggtgaaa
    21001 cgtggcgcgc tgtatattgg ctggagcgca ggcgctaacc ttgcttgccc aactattcgt
    21061 accaccaacg atatgccgat tgtcgatccg caaggtttcg atgcgctaaa tctgttcccg
    21121 ctgcaaatca acccgcactt caccaacgcg ctgccggaag gccataaagg tgaaacccgt
    21181 gagcagcgta ttcgcgaact gctggtcgtc gcgccagaac tgacgattat tggtctaccg
    21241 gaaggtaact ggatcacagt gagtaaaggt cacgctacgc tgggtggccc gaacaccact
    21301 tatgtgttta aggctggtga agaagcggtt ccgctggaag ctggtcaccg tttttaagtg
    21361 aattgccgga tgcatgatcc ggcatcttgt tcatagtgcc tgatgcgacg cttgacgcgt
    21421 cttatcaggc ctacaggatt cacaatatta atactcgtcc cgtccttcat cttcatccgg
    21481 ctgttccagc acactgtaag ccaccgaaca aaacagcgag ttcaggcgct gcatatctcc
    21541 cagtaagcct aaatgtagcg aactggtttc aatgctctgc acgttttgct gatgcaggcg
    21601 atcgacgtgg gcgtgggaat agcggcgatt aagaatgcga aaacgatgtt tgctgcgacg
    21661 caaacgacga gcgctggtga catcgccaga gaagaacacc gacattgcca gttttaaatt
    21721 actgagcaat tgctcataga gcgcatccag ttccttcaag ccatcaagcg aaaatgcccg
    21781 ccgtgctgcc agcgatttat cagcaatttc gctgcccatg cgctcgacga tatcggaagc
    21841 ctgttcaagg ttgagcgaca tttcaataat ctccgcccag cgacgcgact cttcttctgc
    21901 cagttcctct tttggcatcc gcgccagata cagcttaatg gcggtataga gaacgttgat
    21961 atcatctgcc agcttacgca gctctttctc ctgccgtggc tcgccgtgca tcactttatt
    22021 cagcccttcc atcatctgtt ccatggcgtc gccaatgcgc aggttttcgc gcgcggcgtt
    22081 cgccagagca agcgtgggcg tatccagcgc gctgacatcc agatgtttag gccgtagctg
    22141 ggtatccagt tccggttcat cgcgaataat cgttttgcaa aaccgtgcca tcgggtcaac
    22201 aaatggcagc atgaccaggc aacgtacaag gttgtagaag acgtggaaat agatcaccag
    22261 ttccgctttt ggcaatgaca acttccccat tgtctctgcc agcaaatgga caaacggcag
    22321 gataatcagg ctacccacca gcttaaacag caaactaccc agcgcgacac ggcgggctgc
    22381 ggcattggcg gcactgttgt tgagcatcgc cagcaggccg gaaccgaggt tagcaccaat
    22441 caccagacag agcgccacgg ggaaggagat aatgcctgcg gcggtcagag tcgcagtcag
    22501 caatacagca gcaaggctgg agtagctgat aatggcgaac atcgcgccaa tcagcgcatc
    22561 cagcagaata tcgccggtca gcgaggcaaa gatcacctga acgccgtttg cctgggtgat
    22621 cggcgttacg gcctgcacaa tcaactccag cgctagcaaa atcagcccaa gaccaatacc
    22681 gacgcggccc agttgcccgg cgcgtgactg tttgcgtccg aggaaaaaaa tcacgccgat
    22741 aaaaataagt aacggtgaca gccaggataa gtcgaaggtg agaatacgcg ccattagcgc
    22801 cgtcccgaca tctgcaccca gcacaatgac cagagccggt gcgagggcta ccagatcctg
    22861 agcgacaaac gaggtcacca gcatggtggt ggcattactg ctctgtacca gtgcggtaac
    22921 gccgatcccc gcgcaaaagg cgagcggctt tttttcgacg ctccggctaa ggacagtacg
    22981 caaacgcgcg ccgaagacgc gcattacgcc ggttcgaaca atatgagtcc cccagaccag
    23041 cagggcgacg gcagaaagca ggtgaagcag cgttaacacg agttcaggtt ctccttatcg
    23101 ttatatgttc ctgaagcaat tgaatgacca aaggaccgga aacacgctga ctggcatctt
    23161 tactgtgcct tttgacattc gtttttccgg ctaagattat tccagcctgt ttccagtata
    23221 agtgtttcat attaataaag agacagggca ctcattgagc gccctgtttg ttgtaaggaa
    23281 aagtgacaga tttgtgaatc agtccgcgtc ataccccaga ttcggtgcca gccagcgctc
    23341 aacttcggta acgctcatac ctttacggcg ggcataatct tcaacctgat cgcgctgaat
    23401 ttgtgctaca gcgtagtact tgctgtccgg gtggctgaag taccaacccg aaaccgatgc
    23461 accgggccac atggcgaaag attctgtgag tttcatgcca gtgtgttttt ccacttccag
    23521 cagctcccag atggtggctt tttccgtatg ttccgggcag gccggatagc ccggtgccgg
    23581 acggatgccc tggtagtttt cgcggatcag ctcttcgttg ctgaggttct cgttcggcgc
    23641 atagccccag tagactttac gcacacgctc atggagatac tccgcaaagg cttcggctaa
    23701 acggtcggca agcgctttca ccatgatttt gttgtaatca tcgtgctgcg cttcaaaggc
    23761 atcagccagt gcgtcctctt ccagcccgcc agtcacggca aatgcgccga tgtaatctgc
    23821 tttaccagaa agcttcggcg caacgaagtc agcgagacag tagttagcga agcctgtttt
    23881 ttcggtctgt tgacgcagat ggtggctgac gttgatcaca tgggtacgcg tttcgtcacg
    23941 gtagatttca atgtcatcgc ccacacggtt tgccgggaac aggcccacca cgccacgcgg
    24001 attcagcgtt ttctcggcgc ttaatttatc cagcatgtcg ttggcgtctt taaacagccg
    24061 ctgcgcctca acgcccacca cttcatcttc cagaatgcgc ggatacttcc cggccagcga
    24121 ccaggtcata aagaacggtg tccagtcgat gtaattacgc agcgtttcga tgctggcttc
    24181 gacttcctgc acgccgagac ggtgcgccac cggcggcgtg taagcctgcc agtcaaaagc
    24241 gaaatcgtta tcgcgcgccg cttccagcgt gaccggtggt gtgcgcggtt tcttgcgccc
    24301 gtgctgaata cgtacggttt cgtactcctt gcgggtacga gcgacaaaat catcacgctg
    24361 ggtatcggaa agcagcgccg ccaccacacc aacggtacgc gaggcattct gcacatacac
    24421 cgtcgggccg ctgtagttct gctcgatttt caccgccgtg tgcgcttttg aggtcgtcgc
    24481 gccgccaatc aatagcggaa tagtgaagcc ctgacgctcc atctctttcg ccacgttaac
    24541 catctcgtcc agcgacggcg tgataagccc cgaaaggcca atcagatcag cattcacttc
    24601 tttagcggta cggagaattt tttccgcagg caccataacg ccgagatcga caatttcgta
    24661 gttgttacat tgcagcacca caccaacgat atttttaccg atgtcgtgga cgtcgccctt
    24721 cacggtggcg atcaccatct tgccgttggt tttgccctgc tctttgctgg cttcaataaa
    24781 cggttcgagg taggccaccg cctgtttcat gacgcgcgcc gatttgacca cctgtggcag
    24841 gaacattttg ccttcgccaa acagatcgcc gaccacgttc atgccgtcca tcaacgggcc
    24901 ttcaatcact tcaatcgggc gcgtagcctg ctggcgggct tcttcggtat cctgctcgat
    24961 aaactcagta atgcctttga ccagcgagta ttccagacgt ttattcactt cccacgagcg
    25021 ccactccgcc tgctgggcgt tagcggtgtc gtcggttttg ctgccgcgat atttctcggc
    25081 aagctccagt aaacgctcag tgccatcatc gcgacgatta agaatcacat cttccaccgc
    25141 gtcgcgcagt tcagcgggca ggtcgtcgta aatcgccagc tgcccggcat tgacgatccc
    25201 catatccatg ccattgcgaa tagcgtagta gaggaacact gcgtgaatgg cttcgcgcac
    25261 cggatcgttg ccacggaatg agaaagaaac gttagatacg ccgccggaaa tcagcgcgtg
    25321 cggcagttcg cgtttgatgt cttcgcaggc gccgataaag tcctgcgcat agttgttgtg
    25381 ctcttcaatg ccggttgcga ccgcgaagat gttcgggtcg aagatgatat cttctggcgg
    25441 gaagccaacc tcttcggtga ggattttgta cgcccgacgg caaatctcga ttttccgtgc
    25501 gcgggtatcg gcctgtccct gttcgtcaaa ggccattacc accactgccg caccgtaacg
    25561 acgcaagagc ttcgcgtgat ggataaaggc gtcgacgccc tctttcatcg agatagagtt
    25621 aacaatgcct ttgccctgga tacacttcag acctttttca atgacatccc attttgagga
    25681 gtcgatcata atcggcacgc gagcgatatc cggttcaccg gcaatcagat tgagaaaacg
    25741 caccatcgcc gcttcggcgt cgagcatccc ttcatccatg ttgatatcga taatctgcgc
    25801 accgttttcc acctgctggc gggccacatc cagtgcctcg ctgtatttct cttctttgat
    25861 caggcgcttg aacttagcgg aaccggtgac gttggtgcgt tcacccacgt tcacaaacag
    25921 gctatcttcg ccaatgttca gcggctccag gccggacaaa cggcaggcta cggggatttc
    25981 cggcagtttg cgcggcgcta atccttctac tgcacgactc atcgctgcaa tatgttgtgg
    26041 cgtggtgcca cagcagccgc cgacgatatt gagaaaaccc gcttgcgccc attcacgtat
    26101 ctgttttgcc atcgtgtcgg cgtcgagatc gtactcacca aaggcgttgg gtagcccggc
    26161 gttcgggtgc gcggtgacgt agcattccgc aatccgtgac agctcctgca cgtactggcg
    26221 cagttcatcg ggccccagcg cacagttcag gccaaagctc agagcttcgg cgtggcgcaa
    26281 tgagttgtaa aatgcttcgg tggtctgccc ggagagcgtg cgcccggagg cgtcggtgat
    26341 ggtgccggag atcataatcg gcagctcaac gcccagcgct tcaaactccg ttttcaccgc
    26401 aaaaaccgcc gctttggcgt taagggtgtc gaaaacggtt tcaatcagga tcagatccgc
    26461 gccaccttcc accagcgctt tggtggactc tcgataagcc gccaccagcc cgtcaaaagt
    26521 gatattacga aatgccggat catttacgtc cggagaaata gacgccgtgc ggttggtcgg
    26581 gccgagaaca ccggcaacgt agcgcggttt ctccggcgtg cgcgcggtcc actcatcagc
    26641 acaagctcgc gccagtttcg ccgccgcaaa gttgatttcc gccgacaggg attccatctg
    26701 gtaatccgcc atcgcaatgg tcgtggagtt gaaggtgttg gtttcgatga tatccgcgcc
    26761 cgcttcaaag taggcgttgt ggatagcggc gatcacttcc ggtttgctga gcaccagcag
    26821 gtcgttgttg cctttgagat cgcatggcca gtcggcaaag cgttcaccac gaaaatcggc
    26881 ttcgttcagt cgatagctct ggatcatggt gcccataccg ccgtccagca ccagaatacg
    26941 ttcatttaac tgcgcacgca gttgttccac tttgctgctc acacttgctc ccgacacgct
    27001 caacccagat ttaataaaaa ttcaacaaac catactggca taaacgcatc tgtggtaaaa
    27061 gcgaccacca cgcaacatga gatttgttca acattaactc atcggatcag ttcagcaact
    27121 attgcattag ctaacaataa aaatgaaaat gatttccacg atacagaaaa aggaggctgt
    27181 catggtcgca cccattcccg cgaaacgcgg cagaaaaccc gccgctgcca ccgcaccagc
    27241 gactggacag gttcagtctt taacgcgtgg cctgaaatta ctggagtgga ttgccgaatc
    27301 caatggcagt gtggcactca cagagctggc gcaacaagcc gggttaccca attccacgac
    27361 ccaccgcctg ctaaccacga tgcaacagca gggtttcgtg cgtcaggtcg gcgaactggg
    27421 acattgggca atcggcgcac atgcctttat ggtcggcagc agctttctcc agagccgtaa
    27481 tttgttagcg attgttcacc ctatcctgcg caatttaatg gaagagtctg gcgaaacggt
    27541 caatatggcg gtgcttgatc aaagcgatca cgaagcgatt attatcgacc aggtacagtg
    27601 tacgcatctg atgcgaatgt ccgcgcctat cggcggtaaa ttgccgatgc acgcttccgg
    27661 tgcgggtaaa gcctttttag cccaactgag cgaagaacag gtgacgaagc tgctgcaccg
    27721 caaagggtta catgcctata cccacgcaac gctggtgtct cctgtgcatt taaaagaaga
    27781 tctcgcccaa acgcgcaaac ggggttattc atttgacgat gaggaacatg cactggggct
    27841 acgttgcctt gcagcgtgta ttttcgatga gcaccgcgaa ccgtttgccg caatttcaat
    27901 ttccggaccg atttcacgta ttaccgatga ccgcgtgacc gagtttggcg cgatggtgat
    27961 taaagcggcg aaggaagtga cgctggcgta cggtggaatg cgctgacttt ttctggcggg
    28021 cagaggcaat attctgccca tcatacctga gtggcaatag aataagggtg tctgttaatc
    28081 gcattgacgc caaaataact taatgtcata cacttcacac acttttatta actcagcatt
    28141 atttttaaac atcaaaccac ttaatcatta acaaaatcgc aagatctcat tccaccgaaa
    28201 cttttacggc tgcattatga gattg
//
LOCUS       DAPHBJ010000041        28215 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.041, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000041 DAPHBJ010000000
VERSION     DAPHBJ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.041"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(48..1445)
                     /gene="ccp"
                     /locus_tag="RHZ76_001605"
     CDS             complement(48..1445)
                     /gene="ccp"
                     /locus_tag="RHZ76_001605"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HDV2812261.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1850..3499
                     /locus_tag="RHZ76_001606"
     CDS             1850..3499
                     /locus_tag="RHZ76_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDV2812262.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3550..4152)
                     /locus_tag="RHZ76_001607"
     CDS             complement(3550..4152)
                     /locus_tag="RHZ76_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV2812263.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4672..5571
                     /locus_tag="RHZ76_001608"
     CDS             4672..5571
                     /locus_tag="RHZ76_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2812264.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            5620..6633
                     /gene="yhjD"
                     /locus_tag="RHZ76_001609"
     CDS             5620..6633
                     /gene="yhjD"
                     /locus_tag="RHZ76_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HDV2812265.1"
                     /translation="MTQENEIKRPIQDLEHEPIKPLDNSEKGSKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7044..8366
                     /locus_tag="RHZ76_001610"
     CDS             7044..8366
                     /locus_tag="RHZ76_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HDV2812266.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8548..10608)
                     /locus_tag="RHZ76_001611"
     CDS             complement(8548..10608)
                     /locus_tag="RHZ76_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDV2812267.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10678..11445)
                     /gene="pdeH"
                     /locus_tag="RHZ76_001612"
     CDS             complement(10678..11445)
                     /gene="pdeH"
                     /locus_tag="RHZ76_001612"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HDV2812268.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLV
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11677..12606
                     /gene="kdgK"
                     /locus_tag="RHZ76_001613"
     CDS             11677..12606
                     /gene="kdgK"
                     /locus_tag="RHZ76_001613"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HDV2812269.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12702..14198)
                     /locus_tag="RHZ76_001614"
     CDS             complement(12702..14198)
                     /locus_tag="RHZ76_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDV2812270.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAVWDQIMTPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(14419..15705)
                     /gene="dctA"
                     /locus_tag="RHZ76_001615"
     CDS             complement(14419..15705)
                     /gene="dctA"
                     /locus_tag="RHZ76_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HDV2812271.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(15888..17876)
                     /gene="hmsP"
                     /locus_tag="RHZ76_001616"
     CDS             complement(15888..17876)
                     /gene="hmsP"
                     /locus_tag="RHZ76_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HDV2812272.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAKELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPGDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(17958..21430)
                     /gene="bcsC"
                     /locus_tag="RHZ76_001617"
                     /pseudo
     CDS             complement(17958..21430)
                     /gene="bcsC"
                     /locus_tag="RHZ76_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            complement(21412..22518)
                     /gene="bcsZ"
                     /locus_tag="RHZ76_001618"
     CDS             complement(21412..22518)
                     /gene="bcsZ"
                     /locus_tag="RHZ76_001618"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HDV2812273.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKEGLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNLPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22525..24864)
                     /gene="bcsB"
                     /locus_tag="RHZ76_001619"
     CDS             complement(22525..24864)
                     /gene="bcsB"
                     /locus_tag="RHZ76_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HDV2812274.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24875..27493)
                     /gene="bcsA"
                     /locus_tag="RHZ76_001620"
     CDS             complement(24875..27493)
                     /gene="bcsA"
                     /locus_tag="RHZ76_001620"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HDV2812275.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27490..>28215)
                     /gene="bcsQ"
                     /locus_tag="RHZ76_001621"
     CDS             complement(27490..>28215)
                     /gene="bcsQ"
                     /locus_tag="RHZ76_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HDV2812276.1"
                     /translation="RGGVGTTTITAALAWSLQMLGENVLVVDACPDNLLRLSFNVDFT
                     HRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRLSDICSGLQQ
                     LKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQALPDGAHIL
                     INNFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEYRSDALAAEE
                     ILTLANWCLLNYSGLKTPVGSKS"
BASE COUNT         6627 a         7798 c         7122 g         6668 t
ORIGIN      
        1 tcgtaataaa aatcgcccaa acattcctgt tcgggcgatc aaattaatta ttgtttatcc
       61 tgcatatacg gcgtgtacac cccgttcaga ctgtgcagga aagctacgat atcatccaca
      121 tcctcctgcg gcagctcttt gcctacctga tagcgcagca tcagtttcac cgccccgtcc
      181 agcgtcggca cgtcaccgcg atggaagtac ggtgccgtta aagcaacgtt acgtaaaccg
      241 ggtactttct gacgcaattt atcacgctct tctttagtca cattcatacg accaatatcc
      301 gccgccgtaa tttccccaaa gttaaagtct tttttcagcc ccaacggttc aaaggaacgt
      361 ccaccgagaa taataccacc atgacaagtt gcacatttat tatctttaaa taattgatag
      421 ccttttttct gttgcgccgt cagcgcattt tcatctccac gcaaccattt atcaaatggg
      481 gaatccggcg taattaatgt tttctcaaat tcagcaatgg catcagtaat attttcgcca
      541 ctgaaacctt gcggatagac gccgaggaac tgcgctttaa gctgcggatc tttttccagc
      601 ttagcaataa tttcgtccca ggatttcgac gccatttcaa tcgggttcaa cggcggtcca
      661 ccagcctgat cctgcaatgt tgccgcacga ccatcccaga actgctcaac gttaaatact
      721 gagttaaata ccgtcggcgc gttaatcggc ccaactgcgc caccaacacc aatcgatgtt
      781 tttctgccat cgacgccccc cgcattcaac gcatggcaat gagcgcatga aatggtgcta
      841 tcagccgata aacggggatc gtgatacagc gcaaagccca acgccacttt ttgcgcatcg
      901 gtaggcagtt tttgcgggat gggctgcacc ggttcattgc gatgctccgg agcagtatca
      961 ttgctggcgt aatattccgc gcgctgtttt gcaatccagg ccagtatttc cgcccgctct
     1021 tcatcactca ccttacccgc ccagtgtagc gcggtataac gcgttggtgg catagtttca
     1081 tactgcatca cccattcaat cttattcaga tcgctttgcg aaacgggttt atcagccagc
     1141 agtgccgcac gcacggcctc gaggttaaaa gatttatatc caagcttaat gtcgtaatcc
     1201 atcaactgtt tcgcgccagg aatataataa taggcgggta attctgccga aggcgtgtgg
     1261 caatagtcgc atcctttttc gcggagaaag cctaaaacct tattattttc actgacagca
     1321 gatgcctgaa catcggcctg tttactgcgt ttattatcgt ggtaccacac ataaccagat
     1381 aaccctaaat agcaaatcgc gacgccagcc aggccgatcg cggtaatacg tgagaccatt
     1441 ttcattattc ttccctcacg gtggtgagtt attgtgaccc agaaaatttt ccagaatgct
     1501 gcaaacgtct ctgcgagaca aagccatcat aaaaaacagt gagatacgct ttttgatagg
     1561 aatcaatcaa ttacatgaat ataaactatc aataagatag cctgtgccta ttacccagat
     1621 caggaatggc atatcatgat tgcggcgaga gcaggattga atgttctgaa aatgaaaaac
     1681 aacacgaaat ccgtattttt ataaattcca tggatattag tgcgttatat tttttgacag
     1741 gttaataaac gtgatccacc gcacgctttg tcgcccacca ggcggagcga atgactaccc
     1801 ttaaagaaaa gcccgataat tagcgacgaa tttcggaggt tggatcctta tgctcaatca
     1861 gaaaattcaa aaccctaatc cagacgaact gatgatcgaa gtcgatctct gctatgagct
     1921 ggacccgtat gaattaaaac tggatgagat gatcgaggca gaaccggaac ccgagatgat
     1981 tgaagggctg cccgcctctg atgcgctgac gcctgccgat cgctatctcg aactgttcga
     2041 gcatgttcag tcggcgaaaa ttttccccga cagtaaaacc tttcccgact gcgcacccaa
     2101 aatggacccg ctggatattt taatccgcta ccgtaaagtg cgccgtcatc gtgattttga
     2161 cttgcgcaag tttgttgaaa atcacttctg gctgccggag gtctactcca gcgagtatgt
     2221 atcggacccg caaaattccc tgaaagagca tatcgaccag ctgtggccgg tgctaacccg
     2281 cgaaccacag gatcacattc cgtggtcttc tctactggcg ctgccgcagt catatattgt
     2341 cccgggcggc cgttttagcg aaacctacta ttgggactcc tatttcacca tgctggggct
     2401 ggcggaaagt ggtcgggaag atttgctgaa atgcatggcc gataacttcg cctggatgat
     2461 cgaaaactat ggtcacatcc ccaacggcaa ccgcacctat tatttgagcc gatcgcaacc
     2521 accggttttt gcgctgatgg tggagttgtt tgaagaagat ggtgtacgcg gtgcgcgccg
     2581 ctatctcgac caccttaaaa tggaatatgc cttctggatg gacggtgcag aatcgttgat
     2641 ccctaatcag gcctatcgcc atgttgtgcg gatgccggac ggatcgctgc tcaaccgtta
     2701 ttgggacgat cgcgacacgc cgcgtgacga atcctggctt gaggacgttg aaaccgcgaa
     2761 acattctggt cgcccgccca acgaggtgta ccgcgattta cgcgcgggag cggcctcagg
     2821 ttgggattac tcttcccgtt ggctgcgtga tactggtcgt ctggcgagca ttcgtaccac
     2881 ccagttcatc cccatcgatc tgaatgcctt cctgtttaaa ctggagagcg ccatcgccaa
     2941 catctcggcg ctgaaaggcg agaaagagac agaagcgctg ttccgccaga aggccagtgc
     3001 ccgtcgcgat gcggtaaacc gttacctctg ggatgatgaa aacggcatct accgcgatta
     3061 cgactggcga cgcgaacaac tggcgctgtt ttccgctgcc gccattgtgc cgctctatgt
     3121 cggcatggcg aaccatgaac aggccgatcg tctggcaaac gccgtacgca gccggttact
     3181 gacacctggc gggattctgg caagcgagta cgaaaccggt gaacagtggg ataaacccaa
     3241 tggctgggca ccgttacaat ggatggcaat tcagggattt aaaatgtatg gcgatgacct
     3301 tctgggtgat gaaatcgcgc gcagctggct gaaaacggtg aatcagttct atctggaaca
     3361 gcacaaaatg atcgagaaat accatattgc cgatggtgtt ccccgcgaag gcggcggtgg
     3421 cgagtatccg ttgcaggatg ggtttggctg gactaacggt gtggtacgcc gtttaattgg
     3481 tttgtacggc gaaccataat atttttacag ccagccgcta acttcctgct ggctgtaaaa
     3541 ttatcctctt caggaggaga tatttaacat cattgccgcc tgggtgcgat ttttcacttc
     3601 cagacggcga tacagggatt ccagatgcgc ttttaccgtt ccggtactga tattcagcgc
     3661 tctgccgatc tccttatttg attcgcccgc cgctaacatg gttaaaatct cccgctggcg
     3721 ggcgcttaac gatttgagat ctttaatgtc cttttccggc gtcgtccgcc agtctccagg
     3781 cagaaacatc atccccatcg ccgcactatt taccgccaac gcaaatgtct cgacggttga
     3841 atcacgaggc acaatggcca gcacattaaa atggataact tcctgtaacc accgtttatt
     3901 gcaatccgtc gccgtgatta acaccttaac ctcaggaaac tgcaccacgg ttttttgcag
     3961 caaccagtag caaaactcac catcctgatc gccatcgagc ataactaagg cttcagggta
     4021 actttccagc ttttgccata actcgtctgc ctgactggcc ccctgaatac tcactcctgg
     4081 aatacgctgc tgtaaactga ttttcattcc atgaataaat attgactgcc tgtcaaacat
     4141 gactatttgc ataactgaat ctccacctga atacgttaaa aagacttaag tagtggaagg
     4201 gtattacccg cgagaaaaaa taagaattcg ccatttggcg gtggccattc tacagagatg
     4261 acgcgtagaa aatagttacc gatataaata gttacagcta aacgcctgaa attacatgtc
     4321 gagggcacta tttaaaacaa ttttgaggat ttccttatat tggtggttag tacgcatgca
     4381 attaaaaatg aaactccgcg actacaagcc aaaataacaa atggcaatga gacaaaaata
     4441 agcacaaata gccaatacgt cctctattca ctttaaaggg aatcactgaa aaatacgctc
     4501 tgtttaaggg gattcacctt tctcagaaag ctattccgcc cttttcctgc tgagaaatcg
     4561 ccacattcgt catgacaaca ttgtgaaacc cggcattaga tgttagaaaa aacaataaac
     4621 aatgcgatat gcgcgttact gtttcgctgt gaacaacaat cggtcaaaga aatggataaa
     4681 attcacgcaa tgcagttgtt catcaaagtc gcggagctgg aaagtttttc ccgcgcggcg
     4741 gatttctttg ctttgccaaa gggaagtgtt tcgcgccaga tacaggcact ggaacatcaa
     4801 cttggcaccc agcttctcca gcgcactacg cgacgggtca aactcacgcc agaaggcatg
     4861 acctattatc aacgggcaaa agatgtgttg agtaatctca gcgaactgga cggtctgttt
     4921 caacaggatg ccaccagtat cagcggtaaa ttacgcatcg acatcccgtc aggaatcgcg
     4981 aaaaacctgt tactgccgcg tctgtcggaa tttctctatc agcatccggg aattgagctg
     5041 gaactgagta gccataaccg tccggtagat attcttcatg atggttttga ttgcgtgata
     5101 cgcactggcg cgttaccgga agatggcgtt atcgcccgtc ccctcggcaa actgaccatg
     5161 gtcaactgtg ccagtccgca ctatctgacg cgctttggtt atccgcaaag ccccgatgat
     5221 ctgacttcac acgcaatagt gcgttataca ccgcatctgg gtgtacatcc gttaggtttt
     5281 gaggttgcca gcgttaatgg cgtccagtgg tttaagtctg gcggcatgtt gacggtaaac
     5341 agtagcgaaa actatctcgc cgccggtctt gccggtctgg ggattattca gatcccgcgc
     5401 attgccgtgc gcgaagccct gcgtgccggg cggcttattg aagtattacc tggctaccgt
     5461 gccgagccgc tctccctttc gctggtttat ccgcagcgtc gggagctttc ccggcgtgta
     5521 aacctgttta tgcagtggct ggctggcgta atgaaagagt acctggactg accgactata
     5581 ctttttaaga atgaccacga cagaaggaca aaagagcgga tgacgcagga aaacgagatc
     5641 aaacgtccca tccaggatct ggagcacgag ccgattaagc cgttggataa tagcgagaaa
     5701 ggcagcaaag ttagccaggc gctggaaacc gtcaccacca ctgccgaaaa agtccaacgc
     5761 caaccggtta ttgcgcacct gattcgcgcg acagaacgct ttaacgatcg gctgggtaac
     5821 cagtttggtg cggctatcac ctatttctca tttttgtcga tgataccaat tttgatggtg
     5881 tcgtttgccg cagggggctt tgtgctggcc tcccatccga tgttgctaca ggatatcttc
     5941 gacaaaattc tgcaaaacat cagcgatccg acgctagccg ccacgttgaa aaacaccatc
     6001 aacaccgccg ttcagcagcg tacgactgta gggcttgtcg gcctggcggt ggcactttat
     6061 tccggcatca actggatggg taacctgcgt gaagcgattc gtgcccagtc gcgcgatgtc
     6121 tgggaacgct cgccgcaaga tcaggagaag ttctgggtaa aatatctgcg agattttatt
     6181 tcgctgattg gtttgttgat tgcgctgatt gtgacgcttt cgatcacctc ggttgccggt
     6241 tcggcgcagc aaatgattat tagcgccctg cacctgaaca gcattgagtg gctgaaaccg
     6301 acgtggcgat tgattggcct ggcgatttcc atcttcgcca actatctgct tttcttctgg
     6361 atcttctggc ggttgccgcg ccaccgtccg cgcaaaaaag ccctgattcg cggaacattt
     6421 ctggctgcta ttggttttga agtgattaaa atcgtgatga cctacaccct gccatcgttg
     6481 atgaaatccc cctctggcgc agcatttggt tccgtgctgg ggctgatggc atttttctac
     6541 ttcttcgccc gtttgacgct gttttgcgcg gcgtggattg ccaccgccga atataaagac
     6601 gacccgcgaa tgccggggaa aacgcagcct taaaataggc cggatgcggc gtaaacgcct
     6661 tatccggcat acgattttat tcggcctggc tccccgtagg ccggataaga tgcgccagca
     6721 tcgcatccgg ctataatgcg cacataacct cttgaaactc gtccccagag cctcttcagc
     6781 catctatttg ggagcaaaca atttcattcc aactcataac cccagcatat aaatccagtt
     6841 ggtaactttt atttaacctg aaaccagttt tatccactat ttataaaatt atgtgaagca
     6901 tttcatagaa gaaaaatcac tggcctaaac attatcccct ttttgcctga tttttgacca
     6961 tttccgcgat ttgttacaca ttgaaatatc acttttgctg tgcgtaatat ggctattcgt
     7021 tagccaaaaa ataagaaaag attatgcaag caacagccac aacactcgac cacgagcaag
     7081 aatacacgcc gatcaactcg cgtaataaag tccttgtcgc ctctctcatt ggcacagcca
     7141 ttgagttctt cgacttttac atttacgcca ctgcggccgt tattgtgttt ccgcatatct
     7201 tcttcccgca gggcgatcct acggcagcaa cgctacagtc gctcgccacc ttcgccatcg
     7261 ccttcgtcgc gcgccccatt ggctctgccg tttttggtca ttttggcgat cgcgttgggc
     7321 gtaaagcgac gctggtcgcc tcgttgctaa cgatggggat ttcgaccgtg gtgattggtc
     7381 tgctgccggg ctatgccacg attggtattt tcgccccgct gctgctggcg ctggctcgat
     7441 ttggtcaggg tctgggctta ggcggtgaat ggggcggcgc ggcgctgctg gcgactgaaa
     7501 acgccccacc gcgcaaacgt gcactgtatg gctcctttcc gcagctgggc gcaccgattg
     7561 gcttcttctt tgccaatggc actttcttgc tgctttcctg gctactgacc gacgagcagt
     7621 ttatgagctg gggctggcgt gtgccattta tcttctcggc ggtgctggtc attatcggcc
     7681 tgtatgttcg cgtgtcgctg catgagtcgc cggtgtttga gaaagtcgct aaagcgaaaa
     7741 aacaggtgaa gatcccgctg ggtacgctgc tgaccaaaca tgttcgcgta accgtactgg
     7801 gtacgttcat tatgctggca acctatacgc tgttttacat catgacggtc tactctatga
     7861 cctttagtac cgccgccgcg ccagttgggc ttggcctgcc gcgtaacgaa gtgttgtgga
     7921 tgttgatgat ggcagttatt ggttttggcg tgatggtgcc agtcgctgga ttactggctg
     7981 atgcctttgg tcgccgtaaa agcatggtaa tcatcaccac gctgatcatc ctgttcgcgc
     8041 tgttcgcctt taacccactg ctcggttctg gcaacccgat tctggttttt gccttcctgc
     8101 tgctggggtt aagtctgatg ggtctgacct tcgggccaat gggtgcgctg ttaccagagc
     8161 tgtttccgac agaagtgcgt tacaccggag catcgttctc ttacaacgta gcgtcgattc
     8221 tcggggcttc cgttgcgcca tatatcgcag cctggttgca gactaactac gggctaggtg
     8281 cggtggggtt atatctggcg gcgatggctg gcttgacgtt aatcgccctg ctgctgaccc
     8341 atgagacgcg acatcagtcg ttgtaataag agtgaaaagc ctgatgcgct acgcttatca
     8401 ggcctgaatt tccatacaat atattgaatt cgcaaggatt tgtaggccgg ataaggcgtt
     8461 cacgccgcat ccggcatgaa caaagcacac tttgccaaca atctaaatct gtttaattgc
     8521 cggatgtcag acatccggca attatcatca cttcttcatc tgcgacaaaa tcgtccgaca
     8581 ctgattagcc tccccttcgg aaggtgagat cagtgccagt aacgccgccg ccggtgttac
     8641 cagcgttgcc agtgccgcag caacagcacc acgggcaatc agcggtccgg ctttcacccc
     8701 agcctgcgga tttttaaacg tcccccgcac atacagcggc gaacgcagtg tgataatccg
     8761 aattccttta ctctccggat caatagtcaa atccagctgt tccgaagcaa aacttgccgt
     8821 gccggtaaca ttaatcaacg cgttctcagt atcgaaagca aaaatctgcg ggcgcgccac
     8881 gccgttggca atattcagat tcgccgccgc gcagttcacc cgcacctcat catcaccaaa
     8941 tatcgcaccg acaatgtagt tgccgacatt cagcccaaca atctccatca ggttgcggct
     9001 caccagcccg tcattcatca acagtttcag gttgccgtta ctgttgccta aaagtgccgc
     9061 caccgagtta ccgctaccgc gtagttccgc gtcaccgttc atttccccca gcgtcttctg
     9121 catcagttcc acatcgggca tcagttcttt cagtttcagt cgacgagcct gaatatctgc
     9181 ccgcccctgc atcggctttt tatcgccttc cagatgaata ttcgccgcaa tgctgccgcc
     9241 cgccatgcca aatttcagcg gttgcaggcg caggtcagca tttttgagga tgatatgagt
     9301 agaaagatcg ctaatcggca ggctactgcc atgctcaatg cgccgccctt tgaagcgaac
     9361 atcggcatcc ataacgtccc atttatcggt ttcgaagcgg tcataaggca gcactttgcc
     9421 cgcaggctga acgctttttt cgcccttctt ctgttcagac cgtttcgact tttctgcccc
     9481 tttcccggaa tcaacgccaa tcaacggtcc caggtccgcc agccgcaatt gccgcgactc
     9541 gacatcacct tccagttttg gtcgtggctt tccggtggtg tagaccagag aaccgtggat
     9601 atcgctatca ccaattcgcc cattaaaacc gcgataatca aagaccgacg atttttcagt
     9661 gtcgattttc gctaccagcc gaccatccgt ttcaaacggc ggggtatcgg gcagcagaac
     9721 gcccgtcagt tcatagagat cacccagtga atcgccagaa aatttaagcc gtaaatcgac
     9781 accgcccatc ttcattgggt cattcacgac gccatcaaaa gcaacacggg tgttaccaga
     9841 gcggaaatca gcctgtaccg gaaacggcgt cccttcgcca cgcagcgcca gcataccgcc
     9901 tatttttccc gtacccgtga gcggttcacc gttatatcgt ccctgcgcct tcaggccaaa
     9961 aacgtaatcg cccacctttt ctttatccgc tttacctttc gatccagtaa cttcgctgaa
    10021 cggcagcggc ttgcctaagg gatcaacaaa aatctccaga tccgctttgc ttactttgtc
    10081 atcaatggcg atccgccctt gatcgaaaag aatattatcc agccgaaacg accatgccga
    10141 cggctttgca ttcgcgtctt tgttatcatc gttggcaaga ttaaacgtcc agttattgtt
    10201 cttttcagag aggcgaatca ggcgcgcgtc gggcttttcg agcttgatcc acggcagcca
    10261 gaccgttttg gtcagcagcg ccagcggggc cagcgttgcc tctacgcgtg gcaaatgcac
    10321 catcgtgact tcgggaatat ccggtggatt gccaagaatg atgtcttccg cgtgtacatg
    10381 gggccacggc acccagctgc gccagccagt ttcttgtttt tgccgctccc acaccacgcc
    10441 cagatcgcca cggatagcga acggacgatt caactccgca gagactttct ggttgatagt
    10501 cggtttgagt cgattccagt caaaagttgc aatcaaaatg atcgccacga caatcaacaa
    10561 caagaaagcc cctgaaatcg cagcggttat tttgcctgcc ttgctcatcg ttcgcttcct
    10621 catattcttc ctgtgccagt cctaaagata gtccagccag gcggaaaatg aggcagctta
    10681 tagcgccaga accgccgtat tcagcgtttc tatcggtgcc gggcgtgaaa gaaaccagcc
    10741 ttgtgcggcg aatgcgggcg agttctgaac atcacgccac tcttccggcg tttctacgcc
    10801 ctcgacaatc accccgcgac aatagcgatt cattagatgt aaaagctgag aaaagagtgt
    10861 gcgtccttcc ggcgactgac gcagcatcac aaacagttct cgcgcgattt tgatgtagtc
    10921 ataacgcact tcacttagcg cagagaaatt tgccatcccg gtaccaaaat catccagcca
    10981 cagcgggcca aattcacaca tcgaggcaaa ggttgaatct ttcggcagac ggatatgctc
    11041 caccagttcg aaacgcagcc agggaagacg ctcaatctgg cgcaggattt ttggttgctg
    11101 acgcagggcg atgagcgtag ggccatcaat attgaccgat accagcaggc cgtgctctat
    11161 aaagaagtcg gctttttgcg ccagcaaatc aatctgctct ttcacaacct ccatccgatg
    11221 gctgacggtg atttcagtaa aatagcgatc cggcggcagg cgttgcgaag ggttcaaggg
    11281 atgcgtgacc accgttaata gctccacggc cattaaccgc ccgcatgttt gatagatcgg
    11341 ctgccaggtg taagcacgct cacactgcaa ccaaaaacgc cgttcctgca agctctcgat
    11401 gcttgcttca gggttgctta ttcgctggat aacctgcctt atcatcaaag atgtcctgtg
    11461 tttaaaagtg atgctgcccg gactcgacaa agattatcgg cgcgtaaggg cagaacttta
    11521 ccgcctggcc ggggtaaatt tcctcactcc acaacggaat ctttcaggaa tacgttctgg
    11581 ctcaaaaaaa taatggaaca ctgttttaat atggttgacc agcaaaccac cacagcgcaa
    11641 actaacgcta attttttaca gatcaggttc acgactatgt ccaaaaagat tgccgtgatt
    11701 ggcgaatgca tgattgagct ttccgagaaa ggcgcggacg ttaagcgcgg tttcggcggc
    11761 gataccctga acacttccgt ctatatcgcc cgtcaggtcg atcctgcggc attaaccgtt
    11821 cattacgtaa cggcgctggg aacggacagt tttagccagc agatgctgga cgcctggcac
    11881 ggcgagaacg ttgatacttc cctgacccaa cggatggaaa accgtctgcc gggcctttac
    11941 tacattgaaa ccgacagcac cggcgagcgt acgttctact actggcggaa cgaagccgcc
    12001 gccaaattct ggctggagag tgagcagtct gcggcgattt gcgaagagct ggcgaatttc
    12061 gattatctct acctgagcgg gattagcctg gcgatcttaa gcccgaccag ccgcgaaaag
    12121 ctgctttccc tgctgcgcga atgccgcgcc aacggcggaa aagtgatttt cgacaataac
    12181 tatcgtccgc gcctgtgggc cagcaaagaa gagacacagc aggtgtacca acaaatgctg
    12241 gaatgcacgg atatcgcctt cctgacgctg gacgacgaag acgcgctgtg gggtcaacag
    12301 ccggtggaag acgtcattgc gcgcacccat aacgcgggcg tgaaagaagt ggtggtgaaa
    12361 cgcggggcgg attcttgcct ggtgtccatt gctggcgaag ggttagtgga tgttccggcg
    12421 gtgaaactgc cgaaagaaaa agtgatcgat accaccgcag ctggcgactc tttcagtgcc
    12481 ggttatctgg cggtacgtct gacaggcggc agcgcggaag acgcggcgaa acgtgggcac
    12541 ctgaccgcaa gtaccgttat tcagtatcgc ggcgcgatta tcccgcgtga ggcgatgcca
    12601 gcgtaaacgc agcatatgca aaaggctgga taaagtgtta cccctctatc cagctcatcc
    12661 ataaatacct gatggttgtg ctaactcatc accatcaggc attactgtgc aggtggaata
    12721 tccgtcactt caggatgtac gtcatccgtg gcgacagagg tggtcgcagc ggcggtagat
    12781 ggggtcatga tttgatccca gaccgcctgc aacgctttca tgttaaattc cggctcgcct
    12841 ttcggctgca gcagtattaa cgccatatca ttcgacaact gttgacgcag atcctgattt
    12901 aacatctcta ccgtcaggct attaaggaaa tcctgccgca gtttctgata ttgctccggc
    12961 gcgatatcga caacctgatt ttgcaacgaa cgcatccgct gacccatcag aatatcggta
    13021 tcagctcgtg cataggcggc aaacagtttc tgcagctcca gtttcttttg cgccactaac
    13081 gcattgaact cttcttccgg cagaccttta tcgcgaacct tcgccagttc acgcgccacc
    13141 agattaaggt tgctgttcag cttgtcgttt ggtgattcga tgttgatggc acactgcgca
    13201 cgcagataca gcacacggca gtcaaatcca agaccgatgt ctttgctgtt actggcactt
    13261 aacgcttgct gaacatgcca gaacagcgcc tcgcgggcca ggtccgcacg ccagtagcgc
    13321 agcagtgcgg ctgattcacg aatcggctgc cacggcgtat cccacatgat agataaccgg
    13381 tcctgacgca ccgcgtcagt cataatactc accgcttccg cacgcagcgg agaaagcgtc
    13441 ggcaccggag ctggcgtttc acgtttgcct ttcagttcgc caaacgtttt gtttatttgg
    13501 tcgacaaccg agcgcgcatc cacgtttccc accaccagta gcgtcattgc atccggggtg
    13561 taccatttct gatagaaatc tttaattttt tccgcttcaa cgggttgttt cagcggatcg
    13621 gcaggatcgt gacctaacaa ggttgaccct ttcagacgat agcgccacca gccctcttta
    13681 gtatcggcag gccaggttgc caccatgtcc tgactttgca gcgcgtggtt gatggtttct
    13741 ggtgtgatgg tcaatttgcc agtggcattt gccagataag agagcgcttc tttcagcaaa
    13801 tcgttacggt tattgggcaa actcagatta aacagcgtgg tgtcataaga gacaattacc
    13861 ggcggcatcg ggcgtttagg gtcgatcccc tgctgccaca atgaacgcgc ctgtgctgcg
    13921 tcaaggccac cgctttgcgt tagcgcaata cgagggatgg cgtgactgta accgctctgt
    13981 tgtgtacttt cggcgagcga accggtatta accagcaggc gaatttcaac acgatcgctg
    14041 ggacgctggg gggtggtcag cacttgccac tgtaaaccgt tggaaagcgt cccctgttgc
    14101 catgctggat caggctggag cgcatctgcc tgcacatagc cagcagtggc catcatcagc
    14161 aaaccgcccg ctaaaagtcg aatttttgtg ccctgcatgt gaacccctga tcaactatcc
    14221 tggtaataaa aaaactgccc gttgtcggtc agtctaaatg acgttaaaaa cacttcgtgt
    14281 tagaccgcaa gaacatgaaa atgtcacgga agaagtgaaa taaacctgaa ctcacccagg
    14341 tggtaagttc aggcacaggg gtcaattatg cgcaaacacc cgcactcggg gaagggagtg
    14401 cgggcataag tgatgagatt aagaggataa ttcgtgcgtt ttgccatccg gcgcacgatt
    14461 attcagcaca tcgtccagtt ttttgtggtc cagttctttc acccacttag caacgacaat
    14521 ggtcgctacg ccgttaccga ccaggttagt cagcgcacga gcttctgaca taaagcggtc
    14581 gataccgagg atcagcgcca gacccgctac cggcaaatgg cccaccgcag agagcgtcgc
    14641 cgccagcacg ataaagccac tacccgttac ccctgccgcc cctttagaag aaagcagcaa
    14701 cacgattaac agcgtgattt ggtggacgat atccatctga ctgttagtgg cctgggcgat
    14761 aaacaccgcc gccattgtca ggtatatcga tgtgccatca aggttaaacg agtagcctgt
    14821 cgggatgacc agccccacca ccgatttacg gcagccgagt ttctccatct tgtcgagcat
    14881 acgcggcagc gccgactcgg aagatgaagt ccccagtaca atcagcagtt cttcacggat
    14941 gtagcggata aatttgaaga tactgaaacc agtcgcttta gcgattgaac ccaataccag
    15001 caccacaaac aggatacagg taatgtagaa acagataatc agctgcccca gttgcaccag
    15061 tgtgccgacg ccgtatttac cgatggtaaa cgccattgcc ccgaacgcac caataggtgc
    15121 cagacgcatg atcatattga tgatgccgaa gatgacctgc gagaaacttt cgatgacgtt
    15181 aaaaatcagt tggcctttgc tgcccagacg gtggagcgca aaaccaaaca gtacggcaaa
    15241 cagcagcacc tgcagaatgt taccgctggc aaatgcgcca atgacgctcg ccgggatgac
    15301 atccataatg aaggcgacaa tgccctggtc tttcgcctga tcggcgtaaa ccgctaccgc
    15361 tttcgcatca agcgttgccg gatcgacgtt cattccggca ccaggctgca cgacgttaac
    15421 gatgataaga ccaataatca gcgcgatggt actgacaatt tcaaagtaaa gcagtgcgac
    15481 tgcgccggta cgaccgaccg ccttcatgct ttccatgccc gcaatgcccg ttacgacggt
    15541 acaaaagatg acaggagcga tgatcatctt aatgagctta acgaagccgt cgccaagcgg
    15601 tttcatttgc tcgcctattt caggatagaa atggccaagg agaataccaa tggctatcgc
    15661 tgtcaggacc tgaaagtaaa ggcttttaaa cagagaggtt ttcatagggt gtcctttagt
    15721 aaaaccacag gtcttgtaag gttatggggt acctgcggcc ttaaaataac acccagacaa
    15781 catcacagaa atgtacctgg atcataattg aaacaaaaag gttaaaaagt ttgagctggc
    15841 tcgcacaaac cagcactttt taaagttttg taatcagttt ggggtagcta cttttcttcc
    15901 aggtaactct cttcgaagat ttcaataggg agtgggcgag caaaaaggaa gccctgggca
    15961 ataccaacgc ccgcttttgc cagccagtcg cgttgtgctt cagtctccac gccttcggca
    16021 atcatttgta agttcaggct ctgcgccagc atgataattg cagcaatcat gctgctatct
    16081 cccggcaagc cttcaacaaa cattttgtcg attttcagta cgtcgattgg caacgatttc
    16141 atatgctgca gctgacgcag ccctgcgtag cccatgccga aatcatccag cgccacccga
    16201 actccggcat tgcgcagcgg acggaggatt gccaccgcag catgagggtc gtcaatacgt
    16261 cggctttctg tcacttccag aatcagtgtt cccggctgaa tgcgatagcg ggttaacagt
    16321 tccagcatat ccgccaccat attcgggtgc atcagttgca gcgcagagag gtttaccgac
    16381 aagggcagca taatgccgcg ctcttgccag gctgcaagca atcgacagga ctcttccagc
    16441 acccagtgac cgacggtaac catcagccca cagcactcaa tgcgatcgat taagccatcc
    16501 ggcaggtccc aactgccatc cggttgctgg atacgcagta acacttccgc actgaccagt
    16561 ttaccgctgg tcatctcgac ctgtggctgt aaccaaatag caaactgatg attttccagt
    16621 gcattaagga tatcgctctc ttccgtcaac cgcttctggg cggcttccat ctgctgcgga
    16681 tcaaagaact gaatctgatt cttgccttta tggcgagcgg taaatgccgc agaaatagcg
    16741 cgactgtaaa gctgttcggc ggtgagatcg ccgtagaaca tcgccacgcc aatgctacag
    16801 tgcggacgga gttgaatacg ttcaatcggc aggcgctcgc tcatgatagt gagcacttgc
    16861 tgacctaagg tgattgcgtg ccacggttcc tgtacaccgt tggcaatgac agcaaagtca
    16921 taaccgctaa tctgcgcgag gatcatacgt ggcgacagta ccgatttgag tttttccacc
    16981 agcgtcagca gcagaatttc tcgttgcgcc tctttcagca cgcccgcagt atcacgcagg
    17041 gtttcacagg tgatgatcat cagcgcggtg gtttgtttac gcgcgacaac ctgctccagc
    17101 atctccatca gcaaggcttt gttcggcaaa tccgacaccg ggaagcgcat cgcattttcg
    17161 ttctgttctt cataatggcg ctgcagcaat tgctggttga ggttgtaact gcgcaccaac
    17221 ataccgattt catcgtcctg atgcagacgc ggtaatgcca gttggtgacc aacaagctcc
    17281 ttggctggga tggcgttaag ttcgcgagca atattgcgta acggatgcaa aatcaggcgg
    17341 ttaatgcacc agctgatggc gacggtcagg ataagcgaca aaagtaagta aatggtcact
    17401 aacgttgaga gggtgctcat cacgaactta tacatacgga aggaatccgc ctgtagtacc
    17461 agataggcaa ttggctgcgg gtttgccgga cgttcgagcg agtaaacgcc cagcgagatt
    17521 tgaaccggta gctcaaacag gcgagtaacc attaccggca ccgggcgctc tggaataaaa
    17581 cttttacgca gcgcctggaa ctggttaggc agcactacat cggcacggct gaccacgcct
    17641 gccggtttaa tgctggcaag aatagcttcc gcttcgggaa tatcgccttt caaaatagct
    17701 gaagataagg gttcgcggac agagcgagca atgctttcca gttgcgtagc cgtgttatag
    17761 cgattctgct ggaccagatg gaacagcaaa acggtgcaaa aaataaaaac gaacaccagg
    17821 acaacggctg ccaccattgc catctgcttg attgttaacg agcgacttac gcgcaaattg
    17881 tctccccccg gaaatcccag attgccgccc gagtataccc gatcgcagcg gcattaagag
    17941 aggcgctatc tgaaaactta ccagtcggcg taaggtatca gcggctgcgg cggtaaatcc
    18001 atgtcaccct gccatccggc ggcggaataa cgtacgtaga gcaggaaatg gctgggtgcg
    18061 taatctttcg cctgctggat atcaattgcc gtgccaacaa accagttgga agtaacacgt
    18121 cgttcaagta atgcccgcgc cgtgtagccg aagccctgac tgctgccgcc atcgttggat
    18181 tgccgcgcaa cttcttcctg ccagtcggtc gggatcagat tcatcagcgg ataacgcggc
    18241 atggttttgg tgcgtgaatg cgaccacgag ccagacgcac ccagctccca cgaccagttt
    18301 tccgtgcgct cccgccacat caccggtatg gcaaacgaca ggtattcctg cggactgtag
    18361 taaccgccct gaccgagtga gtagccactc agatctttgt cgtaatgcca gatcatgttg
    18421 ttcaggccga ttgtgacgcg gcgattgttc tggttaatga ccttatagta atagcccgtc
    18481 atccagcgca cgcgccagtt atcttcgaca tttttaccgg ttaactggtc gccactaagc
    18541 gatgcccaga cgccgtttgc ttcaccttta tcgtagctca gacttagccc cacaccgtcg
    18601 gcacgtacgc caccccattt tttcccggta ttgctcgggg agtctttttg cccaccaaag
    18661 gccagcaaag aactggagat gggccgacgg tgggcgttaa cggtgtaacc cagcggcccg
    18721 atatcatcgc tgtaactgat gccgccgacc acatccacca cgttgaagcc catcggcgtg
    18781 gtaccgatat cccagctcca gacgtcattt cgccagccga ccgccacgct ggcaccggag
    18841 tccgactggc tgcggttgcc gctacagtcc tgtaatgtac aggtgcccca gttgtcatcc
    18901 catttgccat cggcattagt ggagaaactg ccgacgttca tattgacgaa atcactgcga
    18961 aagaacatcc gcccgtcaga atacggcgca tccacctgca acatggtagt gtgcgctttc
    19021 agatcggagt aaccaccggt gccgctcgaa ccccagtaat cgtgctcaag ggtgacgtta
    19081 agatcctgct ggcgatagag gtccgccgca tcgctgcgca cgccacgttt cagccagtca
    19141 tctttctcgt cgttacgggt cagtcgggta aaggtgtcgt tatcctgcgg acgcgtcgta
    19201 gtcacaccgg atgcgaccat ggcgtctttg taggtttcca gcgcctgcgt tggatcgccc
    19261 gcctgcgctt caaacttcgc accatcacgc agcaccatcg cgctttccat cgacggtggc
    19321 tgagattttg cctgcgggat caacttatta aacgtccgct gcgctgctgc ggtatcgcca
    19381 agctgcgcct gtgccagcgc cacgcgccgc tgtgtgttca gcgaggcgtt atcggtagcg
    19441 ggcagtttcg ccagctggct acgtgccgcc gctttgtcac cggcagcaat atccacttcc
    19501 gtcagaccaa gaatggcgtc ggcgttagct ggctcccgcg tcaggacatt ctgatatgca
    19561 gcgcgggcgg cggtgtaatc acgtcgttgt tgcgcccagt cagccagcgt gaggtcaata
    19621 cgcgtggaag gtggttgctg gcgcagcatc gcttccgctt ctgcctcttt gccgctttct
    19681 cgcaggcggt tagcggtttc cagcacctga tcgctttgca gtcgattaac cagctcctga
    19741 atattgctgt tccactgcgc acgcggcagg ctgttgatat gcgccagcgc cgctctgtcc
    19801 tggtcatgac cagagagata cagcccgtaa gcgtaaacct gctccgggtc attcggcttc
    19861 tgctgcgcca gattgcgcat taacgtatcg gcctggctgc gttgtccggc ctgccagaga
    19921 tcctgcgaaa gtcggtaagt aatccataca ctgccggggt ccagcgccag tcgttgccgc
    19981 tgaagtgctg ccgcctgcgc ccatttgccc tggttttcca gtgcctctgc ctgctgtgcc
    20041 agacggtcgt tttgcaggct gcgttcgata tcatcaatgc tacgccgctg actggcagag
    20101 agcgaggcga taaacgcttc agctttttct ggcgattgct ggcggtaaat atttgccagc
    20161 ccgcgcacgg cgttagtgtt gccgctgtcc atacgcaagg tctgctgata ataacgttcg
    20221 gcggcgggat aatcttttcg cgccatcgcc acatcgccca gccccagcac tgcataactg
    20281 tcggtgttat cgacattacg cgcctgctgg aacaggcgtt ctgcccggtc aggattattg
    20341 gctttcagcg cagcatcgcc ctgctggatc gccagccagt agcggttcac tttcagcaga
    20401 ctattccatt tgtcgttgtt gctgctgtgc gggtccagtg cgagggcttt ttccagattc
    20461 gccactgcat tggcgcgatc gcctttctgt gaatatgcct ggcccagcgc ccccagagcc
    20521 tcactgtctt tcgggttcgc ccgcactgcc tgttgtagtt cgggaatggc tttacccgcc
    20581 ataccagagt ccaccgccgc taaaccttgc gcacgagcgc ggaaagcagg atcggccagc
    20641 tgtttttgct gttctgccag ttgcgattgc gcagccgcca cgctatcgcc atcactaaag
    20701 atcgaaagat attttttcag cgccgacaca ctggcatcgc tgacgggcat gtctttaatc
    20761 tgcccgtacc agattttaga ggccccttcg cgcccggcgt tcgatttcgc catctgttcc
    20821 aggacggcaa aaccttcgtc acggcgatcg ctactaaaca gcaatagcgc cagattgttt
    20881 tgcaggcccg tattgcccgg cgtatccgca ttaatgcgtt ttagctgatt aatcgcttcg
    20941 ccacggcgag ccggaatttt cgccaccgta ctccagtact cgacagcaat gtcaccttcc
    21001 ggcggcgcac cgttgaacag tttgttgtaa ctcgccacag cttcttccgc atgaccggta
    21061 gtcgcctgca atcttgcctg ttgcagtgcc tgacgaccat ccggcgtgga aagtagcacg
    21121 tagtccgcga cgatttatac gcatttgaac tcggcgctaa ctgcgacagc cgatcgagct
    21181 gtttttgcgc gccatcaata tcgccctgac gtaacaaaga acggaaacgg gcggcaacga
    21241 cgtccgggtt attcggatca ataagttcca gccgatataa cgactgttgc accagatctt
    21301 cacgatgggt cgcttcgcct aaccgaactt gctccagcaa ctgttgctga gcggttggtg
    21361 ctgcctcgac catcggcatg acggccagac cgagggaaag cgtgaatatg tttagtgtga
    21421 atttgcgcat tcctggcccc agtcaggtaa taactcacct tttgtcgaga agcggaaacg
    21481 gtgttgatcc cagccttgtc caaacagggt cagcacatag ttgtaatagg catcgctgcc
    21541 gggaaggtta tcggccacgc gctggcgctg aacggcctgc gcatcgcggt tttgtaaaaa
    21601 aggcagcatg gcggcagaaa aaccgaccgg tcctttaccc tgcgctttcc ccgtagccac
    21661 atccactttt tccggcggat aaccgttttt ctcagtgaat gtcgccatcg gtttaaaccg
    21721 gttgagcatc cgcgcttttt gcggatcgct gtcaggcatc atgcctaccc acatgtaaac
    21781 gcggatagcg tcgtagctgc tgatcaatgt tttttcggct tttagctgcc agcctttgtc
    21841 tttctcatag cgcacccagt ctggcgaaaa gcctttcggg gcggtttcca gcaataaacg
    21901 ttgattggtt tcgcgcagcg tagtccacgg cgcgccaaag cgggtgaaat actgcgccag
    21961 cgtcggcggc aggtagctgg ggttaaaacg ccagctgtta tcctcagcaa aacccacttt
    22021 gcccggtaac aacatggaac ccagcccagg caccgtcacc acttcctccc gcgcgatacg
    22081 ttttagcaac gcgctgccga tgtcggtata acgctgctct ttccacaaac gccccgcctc
    22141 cagcaatgac caggccatcc agacatcacc atcggaggcc gaattgctgt ccagcacttc
    22201 ccacttactg ttctctttct tgccccacag ccaggcgggc aaaccttctt ttaaagaacc
    22261 ctgagcgaga ttgttctgcg tccagtcgag aatattatcg aaagctgcac ggtcgttagc
    22321 cgccagggca aagaacatac cgtaactttg cccttcggag gtggtgattt tgcgcgcgtc
    22381 gctggggtcg atgacgcgcc cttcctgact gatgtaatcc tttttaaact gctcccaggc
    22441 aggccaggta caggctgcct gaacactaaa ggcagccagc agcagcatcg tcacgagtcc
    22501 actacgcaac acattcatct tcaattactc gttatccggg ttaagacgac gacgactaat
    22561 aattcgcagc agacgccaca gtacccatgc cagcaatatc acactgattg ccgccagcac
    22621 cgccagcaga atcggatggt ttgccagcgc ataccacaag cgctcgaacc acggcagatg
    22681 acctacgtaa taaacgtcgc caacacgtag gctgttgata ccggactcgc ggatcaccgc
    22741 gaccgaaccg aacatggtgg cgcgtttgcc gctatcgttc accgcatcgt taagcatttc
    22801 ataaccgcgt gggctatctg ccagcagcgc aatcacgctg cgctggtcgt tatacggcga
    22861 ctggaagcca atcaccgccg ccatcgcacc ggaagaggtc agcgttgacc gggtttctgc
    22921 cgcgcgatcg ctctcgtccg gtacaatgcc ggggaacggg gtctggcgca tcggtgtttt
    22981 cacccagctt tcggtcgcct gcaccaatag gtcgatctgc ttatcgtctt tcagtttgtc
    23041 cgggatacca ccgatgatca tgatgtcggc atctttgccc tgaatggtgc tgccatcatc
    23101 ggtcaccgtc agattaatcg ccgggaagcc cgtctgtgcg ccgataaaac caacagtatt
    23161 cagcaacgtt tccatctgtg cttcgttagg cgctttcggc atcacggtga tggtttgcga
    23221 cagatccgcc atccggctga atgggaagcc cgcgttagca aaggcgcgta gatccggcat
    23281 cgggatgaag tggtaatact tcgagaagtc gatggtggaa tcgtcaccaa tcaccacatg
    23341 attctgcacc ggctggaagg taatacagtt atctaccgaa ccgcctggca tcgggttcat
    23401 atactcaaag tcgaagcgca gctggttggt cgcgcccagt ttcagcgccg gaatagagac
    23461 atctgttttg ccatccagca aaccttgtaa taccggaatc cgcagcagca ggcggttcgc
    23521 ctcctgtttg ctgctcaggt tgaaggattg caggaactgg ttattcaggc tgatatccat
    23581 ccgcgaactg tctttcaccg gcggcatggt gtagcgatag ttgatatcca tatcaatgcc
    23641 ggtactgcgc atcaggtaga gatccggtgg caggtttagc gaaacgttaa tcgctgctgg
    23701 ctcaagaccg ctggattgta actgttcttc ataggttttc agttcaccaa aggtgaccgg
    23761 acgatcggta cgtacccagt tcggcgcatc gtaaggctta cgcggtagca gcggtttcac
    23821 ttcattcact accacgcttt caccacggaa cagaatgtta ccctgagcga tacctttcgc
    23881 tgcctgcaac aggtctttgt cgtcacgacc aaacaccacc agcagtttga cgtaaggatt
    23941 ctgcggatgg ttaatcatct caatcaccgg ggcttttacc gccggatgat cgcgcaggaa
    24001 gtccggccgt ttgtcgttgg ttgcaaagac aatggcatta cgatccggca gttggttata
    24061 gagcaccggg aagttctgcc cacgccagcc agaacgcgaa ccaaaccacg aggcgacaat
    24121 ggcagaggct tgttgcagcc caacatccgg cgcacccgca aagaccatcg gcaaggtgtt
    24181 agtacggtta tcgcgcgggt caaagaatgg caccgggaag tgtgacaggt cattcttcac
    24241 attcagggtc tgataggtca gatccagtcc actgctccgc ccaacatcca gccaaagcgt
    24301 ggtgctggcc gggttttcgc acacgtcctg ataatggccg acaaactcca gccgtacacg
    24361 gttgaagtcg gtaataaaca gtgggttaat gggcatttgc gccagcgttt ttttacccaa
    24421 ctgttctttg gtcactggca gcacgcccat cagttcatca ttgagataaa cctttaactg
    24481 cgactggaca ggcagtaacg atggcgatgg ggtgtattcg aggttgagca tcgccttcgt
    24541 caccacttca tcgctgcgca taccaaactc aatgctgccg ttcgggttaa tgccacgtag
    24601 caccatgctg cccggcggcg gtgcaatttg cgcaaaggtc agcttcacat cacgcgaagg
    24661 accgttctgc gccacgactg gcgcatcagc gccctgcacg cccggcatca cttgccccac
    24721 ctgcggattt tgttccgtct gggcggctac agctggctca gcattgatca gtggttgcgt
    24781 tgctggcgtc gcctgcgtca tgaaagaggg gaacgcactc atccccatag ccactgcaca
    24841 aatccagaat agttttcttt tcatcgcgtt atcatcattg ttgagccaaa gcctgatccg
    24901 atggttgtgc cgtttcgctc cgctccgggc ggcgcggaat aaacgataca acccaggaaa
    24961 ccagagaagt cagcacacgg aatatgccct tcaccgaaga aggcgcaaac tccgccagat
    25021 ggcggtagcc acggaagccg agcttcagaa tatccagcag actttccagc ggcttatctt
    25081 ccggatagct gtcctgccag agcgcccatg tatccgcacg ggcaaacgta cactgcacaa
    25141 aatcgatatg ttgctgggtg gtgagcggca ttaatttcag cccaacttca ttacccatca
    25201 cgcgcgccac ctgggtcggg aagacgtatt cctgctgacc gcgtttaagc aacagattca
    25261 ctttctgccc ttccagaatc tgcgcctgac cgttgatctt gatccccaaa ccaccgtcgg
    25321 agaaatcctg aacggtacac gagaagaggt gaccatcttc gcgggcaatt gccgcgggca
    25381 tcgtcatctc cacgcggtgc gatcggcgta cctgtttgct ttctaccgat accgcaactg
    25441 cgccgccaag aacaatcagg ttgtagaata cccacaccat actgacgacc acggtgagca
    25501 tctcggttgg cgggccatag aagtagcgcc agatgcctac cgcaacgccc accaggttga
    25561 gcaggacaag gaagatgtag ggccgcgaga tcacccagtc gacgtactct tcttccacca
    25621 gtccaccttt ggcggtgacg ttaaatttgc ctttgtgcgg gttaatcagc gccaccagcg
    25681 tcggtggtgc gatataccac gccagcaccg tttcgtagat ttcactccag aaagagtggc
    25741 gatatttgcc ctggatcttg gagttggtca ggctggcatg gatcatatgc ggcagcacga
    25801 atagggcgat catcaacgct ggcgcataga tgatgtaggc atgaagcagc aggaacgcca
    25861 gcggcgcagt caggaagatc agccgtggaa tgcccgacaa gaagtggaac atggcgttga
    25921 cgtaacatag ccgctgagca aacttcagcc ctttaccggt gagcgggtta tcgagacgga
    25981 agatttgtac catcccgcgc gcccagcgaa tacgctgacc gatatgcgcc gacagacttt
    26041 cggtcgccag ccccgccgcc tgcggaatac gcatatacgc ggaggtatag ccacgacggt
    26101 gcaaccgcag agaagtatgc gcatcttcag tcacggtttc gacagcaatg ccgccaattt
    26161 catccagcgg cttacgacga atcaccgcac aggaaccgca gaagaaagtg gcgtcccaca
    26221 tatcgttgcc atcctgcacc agaccataga acagcgtgcc ttcgttcggt gttttacgaa
    26281 aacgccccag gttgcgttca aacgggtccg gtgagaagaa gtggtgcggc gtctgcatca
    26341 tcgccagctg tttttctttc aggaaccagc ccatggtcat ttgcaagaac gatcgcgttg
    26401 gtacgtggtc gcagtcgaaa atcgacacga actcgccttt ggcatatttc agcgcattgt
    26461 tgatgttgcc tgctttcgca tgttcatgag tggtgcgggc gatatatttc acccccacgt
    26521 tttgcgcaaa ctggcgaaac tcttccctgc cgccgtcatc aaggatccag atattcagct
    26581 tatctttcgg ccagtcgata cccagcgagg cgtaaatggt atttttcacc acgttgagat
    26641 cttcgttgta agtcgggaca aagatatcca ccgacggcca cagcgacata tctttcggca
    26701 atggcaccgg ctgacgattc agcggccata ctacctggaa gtagccgagc accagcacaa
    26761 tccacgcgta cgtttcagcg aagagcagaa taagcccgca caccaggctg accggatcgt
    26821 cccagttcag cgtagaggtg taacgccacc agatataacg gcaagaaacg gtcagcgaca
    26881 gcacaatcaa cattagcgcc gagaagcgcc ccggcatccg ccgtacgatc agcgctaccc
    26941 cccaaagcag catcaggaag ataaactgcg ccagcgggtt aaacggctga gtaacgcaga
    27001 ttaacgccag aatcagcgag aaggtgacga tgataccgag gatcaaccgc cgcgccccgg
    27061 cactcaaatg accgagctct tttttctcat caagatgctg tgttttatgg ctaacgcgct
    27121 caggcagctc gttcatccat tgatggtaac gtccacgaat attttgcaga cctgaaaatg
    27181 cccgcctgcg cggtttcggc gtttctttgc gcgatgcacc gatcaataac cagcatgttt
    27241 gaatgagata acggaccggg tccagcggac gcggacgcga ggcgttgata tgcggataca
    27301 ggtttttatg ttctgcgcga atacgctgcc agcgcgggtg ctccagcgga ataaaaatcc
    27361 aggccaggat catccagaaa cagccgagcg tcgcgctgaa agccgacgca ccgtggcgac
    27421 gataatcgcg ataacgcccg atcagccgcg cgttgaccgg cgggattagc aaccaccggg
    27481 tcaggatact catgatttac tcccgactgg cgttttcagc ccggaatagt tcaacaggca
    27541 ccagttcgcc agcgtcagta tctcttcagc cgccagcgca tcactgcgat attcacctac
    27601 tggttgctta gccgccaggc attcagccat cgcttcatca cgatgaatga gcatcggcag
    27661 taatcggcgc tggctttgca accaaagctg gtaaatatcg tcctgaacct gactgccaat
    27721 acggaagtta ttaatcaaaa tatgtgcgcc atccggcagc gcttgctgat gcagtcggat
    27781 atggcagttg gcatccacat tgacgattgc cagcgagtga tcgcacaaac tcagcagctg
    27841 gtgggttatc tgcgaggcat cacgcggtaa gtcgattaaa atccactggt aacgcccgct
    27901 ggcttttagt tgctgtaagc cggagcaaat atcgctcagc cgggtttgcc agtgctgtgg
    27961 attttcttgt tcttcaatgg ataactgacc aaaaggcagc aaatcgagct gcgaggtgta
    28021 gcgcaacccg gcgtcacgcc agtcctggcc atccagcatc gctctggccc agccctgacg
    28081 gtgggtaaaa tcaacgttaa atgacaggcg caacaagttg tccgggcagg catcgaccac
    28141 caggacattt tctcccaaca tttgtaatga ccaggctaat gcggcggtga tggttgttgt
    28201 ccccacgcct ccccg
//
LOCUS       DAPHBJ010000042        28147 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.042, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000042 DAPHBJ010000000
VERSION     DAPHBJ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.042"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..622
                     /locus_tag="RHZ76_001622"
     CDS             <1..622
                     /locus_tag="RHZ76_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDV2812277.1"
                     /translation="VATAGLAWGAKYLEITATKYDSPPMYVAIGLLLLCMYGLSKDIN
                     KINAAIAGVIYLFLLSLVAIVVASLVPVYAIIIVFSTAGAMFLISMLAGLLFNVDPGS
                     HRFIIMMTLTGLALVIIVNAALMSERPIWVISCLMIVLWPGIISHGRNKLLELAGKCH
                     SEELWSPVRCAFTGALTLYYYFIGFFGILAAIAITLVWQRHTRFFH"
     gene            complement(658..1614)
                     /locus_tag="RHZ76_001623"
     CDS             complement(658..1614)
                     /locus_tag="RHZ76_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HDV2812278.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHITKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1614..2855)
                     /gene="clsB"
                     /locus_tag="RHZ76_001624"
     CDS             complement(1614..2855)
                     /gene="clsB"
                     /locus_tag="RHZ76_001624"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HDV2812279.1"
                     /translation="MKCSWREGNKIELLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2852..3613)
                     /locus_tag="RHZ76_001625"
     CDS             complement(2852..3613)
                     /locus_tag="RHZ76_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDV2812280.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLQTWRHLSDHAPLSAEIHL"
     gene            3746..4156
                     /locus_tag="RHZ76_001626"
     CDS             3746..4156
                     /locus_tag="RHZ76_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HDV2812281.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(4118..5224)
                     /locus_tag="RHZ76_001627"
     CDS             complement(4118..5224)
                     /locus_tag="RHZ76_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2812282.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     NASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(5235..6368)
                     /locus_tag="RHZ76_001628"
     CDS             complement(5235..6368)
                     /locus_tag="RHZ76_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2812283.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6361..8097)
                     /locus_tag="RHZ76_001629"
     CDS             complement(6361..8097)
                     /locus_tag="RHZ76_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2812284.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALQLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(8090..9085)
                     /gene="hlyD"
                     /locus_tag="RHZ76_001630"
     CDS             complement(8090..9085)
                     /gene="hlyD"
                     /locus_tag="RHZ76_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HDV2812285.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(9088..9759)
                     /gene="cecR"
                     /locus_tag="RHZ76_001631"
     CDS             complement(9088..9759)
                     /gene="cecR"
                     /locus_tag="RHZ76_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HDV2812286.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9988..11352
                     /gene="rhlE"
                     /locus_tag="RHZ76_001632"
     CDS             9988..11352
                     /gene="rhlE"
                     /locus_tag="RHZ76_001632"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HDV2812287.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(11584..12066)
                     /gene="ybiA"
                     /locus_tag="RHZ76_001633"
     CDS             complement(11584..12066)
                     /gene="ybiA"
                     /locus_tag="RHZ76_001633"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HDV2812288.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGHGKGKNRLGYLLMELREQLAIEK
                     "
     gene            12186..14336
                     /gene="dinG"
                     /locus_tag="RHZ76_001634"
     CDS             12186..14336
                     /gene="dinG"
                     /locus_tag="RHZ76_001634"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HDV2812289.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTVPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            14364..15326
                     /gene="ybiB"
                     /locus_tag="RHZ76_001635"
     CDS             14364..15326
                     /gene="ybiB"
                     /locus_tag="RHZ76_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HDV2812290.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            15467..16552
                     /gene="hcxB"
                     /locus_tag="RHZ76_001636"
     CDS             15467..16552
                     /gene="hcxB"
                     /locus_tag="RHZ76_001636"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HDV2812291.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(16781..17041)
                     /locus_tag="RHZ76_001637"
     CDS             complement(16781..17041)
                     /locus_tag="RHZ76_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV2812292.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(17306..17572)
                     /gene="ybiI"
                     /locus_tag="RHZ76_001638"
     CDS             complement(17306..17572)
                     /gene="ybiI"
                     /locus_tag="RHZ76_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HDV2812293.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(17646..18323)
                     /gene="ybiX"
                     /locus_tag="RHZ76_001639"
     CDS             complement(17646..18323)
                     /gene="ybiX"
                     /locus_tag="RHZ76_001639"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HDV2812294.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(18365..20647)
                     /locus_tag="RHZ76_001640"
     CDS             complement(18365..20647)
                     /locus_tag="RHZ76_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HDV2812295.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADISNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPTGVAKGSPVTTVDTAK
                     SGNLMNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(20912..21172)
                     /locus_tag="RHZ76_001641"
     CDS             complement(20912..21172)
                     /locus_tag="RHZ76_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV2812296.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            21448..22374
                     /gene="rlmF"
                     /locus_tag="RHZ76_001642"
     CDS             21448..22374
                     /gene="rlmF"
                     /locus_tag="RHZ76_001642"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HDV2812297.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISSNPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(22371..24596)
                     /gene="ybiO"
                     /locus_tag="RHZ76_001643"
     CDS             complement(22371..24596)
                     /gene="ybiO"
                     /locus_tag="RHZ76_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HDV2812298.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWRVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(24857..25579)
                     /gene="glnQ"
                     /locus_tag="RHZ76_001644"
     CDS             complement(24857..25579)
                     /gene="glnQ"
                     /locus_tag="RHZ76_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HDV2812299.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(25576..26235)
                     /gene="glnP"
                     /locus_tag="RHZ76_001645"
     CDS             complement(25576..26235)
                     /gene="glnP"
                     /locus_tag="RHZ76_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HDV2812300.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(26374..27120)
                     /gene="glnH"
                     /locus_tag="RHZ76_001646"
     CDS             complement(26374..27120)
                     /gene="glnH"
                     /locus_tag="RHZ76_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HDV2812301.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(27524..28027)
                     /gene="dps"
                     /locus_tag="RHZ76_001647"
                     /gene_synonym="pexB"
     CDS             complement(27524..28027)
                     /gene="dps"
                     /locus_tag="RHZ76_001647"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HDV2812302.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
BASE COUNT         6660 a         7323 c         7495 g         6669 t
ORIGIN      
        1 ggtggcaacc gcaggactag cctggggagc taagtatctc gaaattacgg caaccaaata
       61 tgattcacca ccaatgtatg tcgccatagg gttattatta ctttgtatgt atggcttaag
      121 taaggatatc aacaagataa atgccgccat cgcgggcgta atatatctgt ttttactctc
      181 tttggtggcg attgtcgttg caagtttagt tcctgtatat gccattatca tcgtgttcag
      241 cactgcgggc gcgatgtttt taatcagtat gctggccggt ttattattta atgttgatcc
      301 tggttctcac cgttttatca ttatgatgac gttgacaggg ttggccctgg taatcatcgt
      361 gaatgcggca ttaatgagtg aacggcccat ttgggtaata agttgcttaa tgattgtgtt
      421 atggccaggc attatctcgc atggacgaaa taagctcctt gaactggcag gaaaatgcca
      481 tagtgaagag ttgtggagtc cggttcgttg cgcttttaca ggtgcattaa cactctatta
      541 ctattttatc ggcttctttg ggatacttgc cgcgatagct ataacgcttg tctggcaaag
      601 gcatacgcgt ttttttcatt agtcgtaaat gcagatacta aagaacttaa ctgaacttca
      661 catcgccgct tcatttttcg ctcgtagctt tttcgcctgg ctttccagca acaggtaaca
      721 gatcagcgcc agcagcagtg ggataaagta atacagcaca cggtaagcga gcagggcggc
      781 gataattgtg cctttggtga tatgctcccc agccagtagc gcgataaaca ccgcttccag
      841 cacaccaatt cccgccggaa tatgcacgat gacgcctgca atactactaa ccagcaacac
      901 gcccagtaca aagaaatagt taacgctttg accaagtaac agccagataa tcgcccccat
      961 taccatccag ttaacgctgg aaatcagcat ctgggcgagg gcgaatttcc atgaaggcag
     1021 taccagcttt tgccctttaa tggtcatatg gcggtgcttc gcgaaagcgc aaaaccacaa
     1081 ataaaccgcg ataatcatca gtaagccaat gccgagaata cgcagcgtag tttgatcgac
     1141 ataccagtga tccggcaact ccaccacgcc tgcggtaaag ataatccccg ccagcaaaat
     1201 atagcccagc cagttggtgg tgatactgag cgagaaaatc cgcgtaatag tgctaccggg
     1261 caaccccaga cgagagtaca aacgatagcg cataccaatg ccgccgaccc aggtactgag
     1321 tgtcaggttg aaggcgtagc agataaacga caccagcatc acctggcgct tcgccagttt
     1381 gtgaccgcag taaaagcggg cgagcaggtc atagcagccg taaatcagat aactgacgac
     1441 caccagcccg accgcactga gcagcgcaac gcgattgtag tcacggatga ccttccagac
     1501 ctcttcccag tccacctttt tggcgtagac caccagcaac acaatcaccg cgataaaaaa
     1561 cagccaggtg aggatcttct ttgctaagcg ccagcgcggg tgtgatttac tcatcagggt
     1621 tttacccccg tgttttcagt ttctacccga tcctgcgttt ccattgtcgg ttgtgcgggg
     1681 ggatcaacct gcgtcagacg tggtgtgtgt gccggaagcc agccaaccag cgccgggaag
     1741 tggcgtaaaa agtggaacgc cagcacgctt ttggtcaggt tccaccaggt gcgtttcggc
     1801 agcatggtct catcgacctg ctgacaatct gcggcaataa tgccgttcag attatcgcgc
     1861 agcgtctggt taaaatgacg atcgtggatg atgacatttg cttcgagatt cagtgacaaa
     1921 ctgagcggat cgagattact ggaccctact gtcgcccagt gatcgtccat caatgccact
     1981 ttgccgtgga gcgggcggcg gcggtactca aatacctgaa cgccgccttt aaccagatag
     2041 ttatacagca agcgcgcacc gactctgaca atcggcatat ccggttcgcc ctgaatgatc
     2101 agtttgatcc gcaccccgcg ccgtgccgct ttacgcaagg cgtgtaaaaa tcgatagccg
     2161 gggaagaagt aggcgttggc gataatgact tctcgctgcg cctgagtgag cattttcaga
     2221 tagtgacgtt caatatcatc gcgatgttct tcgttatcgc gccagaccag caatacctgc
     2281 gcttctcccg gctggcggtt ctcttccgct ttgtgatgac gtcgccacca gcgtcgtgcc
     2341 gcgctctgtc caggcaggtt ttccagctca aactggagaa tatcttcgac aatcggccct
     2401 tcaaggcgta ccgcgtaatc ctgtttagcc tctgggccgt agctggacat atgctcggcg
     2461 gagtaattca gcccgccaat aaaggcgata cgcgcgtcga tcaccacaat tttgcgatgc
     2521 atccggcgaa acacattggt gcgcatacca aaaaggcgag ggcggggatc gtagtagcga
     2581 aataccacgc cagctgccgt cagttcattg acaaactcat cgctgagatc cggcgaaccg
     2641 tagccatcca gcaagacttc cgctttaacc ccgcgttgcg ctgctgccag tagtgccgca
     2701 tgcagctgtt tgccgacgtc atcctcaaac cagataaacg tttcaagaat gatgcgttct
     2761 tgtgcctcgc caatcgcctt aaacaccgcg ggataatatt gctcgccgtt ttccagcaac
     2821 tcgatcttat tgccttcacg ccagctacat ttcataaatg aatctccgca cttaaagggg
     2881 catgatcaga aaggtgtcgc catgtctgca gcggcaacgc ggttggcgcg ctggcgctgg
     2941 catttttgac gtagatcctg tccagacgta gtagaggaaa ttgcaccgga aacgtgcgcg
     3001 ccgggcgtcc gtgggcgcgg gtaaatatct catccagccc ggcctgcact tttaacggat
     3061 gattagcttt ttgccgccag tcgttgaaat cacccgccac caatactggt tcgccgtccg
     3121 gcagctcatt gacccattcg gcgagcatcg caagctgcgc ctgacggtgc gcctcacgca
     3181 ggcccagatg tacgcacatc acatgaatcg cttttccggt catcggcggc acaatgcggc
     3241 agtagagcac gccgcgcttt tccgcaccat cgaccgaaac atcgcgattc tcataatgtt
     3301 caatgggata acgcgacagt acggcgttgc cgtgatgccc ttccgggtat acggcattgc
     3361 gaccgtaggc aaaatcgctc cacatagtgt cggcgagaaa ctcgtagtgc gaggtatcgg
     3421 gccagttttc cacatgcagc ggatgaactt cgtgcgcgcc catcacttcc tgcaggcaaa
     3481 caatatcggc gctgacggta cgcacggcat cgcgaagttc cggcaaaatg aagcgtcggt
     3541 taaacgcggt aaagcctttg tgaatattga tggtgagcac cttgaacgaa aattgttgtg
     3601 tttgatcggg cataattttc ctgtctttgc ctcttatctc attgaaatag tgtagtcggc
     3661 gtcacaaaaa ggtgcggtct tacggaattt tccgtaaagt tcggtactct gagtaagtag
     3721 agataaattc ttcaggagag aagccatgaa gtggcaacaa cgtgttcgtg tcgcaacggg
     3781 tctaagttgc tggcagatta tgttgcattt actggtagtg gcgctgctgg tggtgggctg
     3841 gatgagtaag actctggttc acgtcggcgt gggattatgc gcactgtatt gtgtcacggt
     3901 agtgatgatg ctggtgtttc agcgccaccc cgagcaacgc tggcgtgagg tggcagacgt
     3961 gctggaagag ctgaccacga cctggtattt tggcgcagcg ctgattgtgc tgtggctgtt
     4021 gtcccgcgta ctggaaaaca actttttgct ggcaattgca gggctggcga tccttgctgg
     4081 cccggcggta gtgtctttgc tggcgaaaga taagaagtta catcacctta cgtctaaaca
     4141 tcgcgtacgc cgctgaccct gtcgtggccg ttatcaccag tagcggccac aaactattcc
     4201 acacaatatc cagactcgca ttcttcaaat aaatctgctt ggtaatgtcc gtaaagtggc
     4261 gaatagggtt aatccacgtc aggttttgca gccatactgg catgttttcc accggcgaaa
     4321 cgtaaccgga aaggagaatg gcgggcatca taaaaacaaa cacgccgata aacgcctgct
     4381 gttgtgtcga gcagagtgat gaaatcaaca gaccgaatcc caccagcgat aaaccgtaaa
     4441 tcaccatcgt aaagtagaac agcgccagcg atccggcgaa ggggatttga tacgcccaga
     4501 taccaatcgc cagcacaatg gtggcctgga aggtggcgac aattaacgcc ggtacggctt
     4561 tgccgatgaa gatctgccag gtggtgagcg gcgaaaccag tagctgatcg agcgtacctt
     4621 gttcacgttc gcgggcgacg gaaagtgaag tgacgatcat tacgccgata gtggtgatca
     4681 tggcgatcag cgacggcacc acaaaccatt tgtagtcaag attcgggtta taccagttgc
     4741 gtaccaccag ctcgctgttg ttaggtttcg gttttccttc cagcagctcc tgctgataat
     4801 ttttgacgat ctgttgcagg tagttggcgg caatttgcgc actgttggag ttacgcccgt
     4861 cgaggatcaa ctgcaacggc gcggtctgga aggtatccag tttgcgcgag aagtcagccg
     4921 ggaaacgcac cagtagtaac gccttttgtg tgtcgatggt tgggcggatc tcctgtgggc
     4981 ttttcagcag cagcacatgg gtaaaggcgc tggcgcgggc aaaacgttgg gtcagctcca
     5041 ccgaatgctc gccgttatct tcatcgtaga tggcgatggt ggcgttagtc acttccagcg
     5101 tggcggcgaa cgggaacagg atcacctgaa taagcacagg taaaatcaga atcgcgcggg
     5161 tttgtggttc gcgcagcaac gactgcaact ctttgcggat taacgtccat aagcgatgaa
     5221 acatgctctt ctccctaatc cagccgacgt ttggttttca gccacgtcag gccgataaac
     5281 atcaccgccg aagcgatcaa aaacagcacg tttaccacca gcaccactgg aatattcccg
     5341 gcgaggaaca ggctttgcag ggtgctgacg aaataacgag cgggaataat gtacgtcacc
     5401 gcgcggatca ccgcgggcat actgtcgatc tgaaaaataa agccggaaag cataatcgac
     5461 ggcaggaacg cggcgttcag ggcgacctga gcggcattga actggttgcg ggtaatcgtg
     5521 gaaatcagca gccccatccc cagggtactg agtaaaaaca ggctggagat aaaaaacaga
     5581 ataagcagcg aaccgcgata cggcacgccg agaataaaca ctgacaccag catacacagc
     5641 agcatcgcca gcatcccgag aaagtaataa gggatcagct tacacagcag cagttccgtg
     5701 cgggtaatct ccgtagagag cagagcctcc atggtgccgc gttcccattc tcgcgccacc
     5761 accagcgagg tgagaatcgc gccgatgacc gtcatgataa tggtcaccgc acccgggata
     5821 ataaagtgct ggcttatcgc cgccgggtta aaccagtagc gggtttgtac atcaataagc
     5881 ggttcaaaag tctgcccgtt gtcctccgct cgctgcattt gccagatctg ccagatccct
     5941 tcgacatacc cctgtacaaa gttagcggta ttcggctcac tgccgtcggt gatcacctga
     6001 atcggtgcgg tggcgttggc gcgctccatc tgttccgcaa aatccaccgg aataaccacc
     6061 agaccgcgaa ttttccccgc ctgcattttg gcgatcagtt cctgacggtt atcgctgatg
     6121 gtggcgtcga tgtagggcga accggtcatg gtgtgggtga aatccagcgc cgcttcgcta
     6181 cgctgttcca gtaaaatccc gacccgcagc ttgctggagt cgaggttaat gccgtaacca
     6241 aaaataaaca gcagtagcag cgggatcact accgcaatca gccagctact cggatcgcga
     6301 acgatctgcc gcgtctcttt aacgcacagc gcccgtacgc gacgccagga caggatcggg
     6361 ttactcattg ctatgctcct tatcccagtc gtggatcaac tgaataaagg cttgctccat
     6421 ggtgggatcg ggttgctcgt cgttggccga ctgcgctttc aaatcatccg gcgtgccgct
     6481 ggcaattaat ttcccacggt acaccaggcc gatgcggtcg caatattctg cttcatccat
     6541 aaagtgagtg gtgaccatca ccgtgacgcc tttctctacc atgctgttga tatgcagcca
     6601 aaattcgcgg cgggtgaggg gatcaacgcc ggacgttggt tcgtcgagaa acagaatgtc
     6661 cggttcatgc atcagcgaac aggccagcgc cagccgctgt ttaaaaccta atggcagttc
     6721 atcggtcgcg tgggaggcga tacttttcag gccaaatgcc tcgctcatac gggagatttt
     6781 ttcgttctgc gcccgaccgc gtaagccata cacaccagag aaaaagcgta aattctgttc
     6841 gaccgtcagg ttgccgtaga gcgaaaattt ttgcgccata tagccgagat gctggcgcgc
     6901 tttaccggaa ctctctttca gatccatccc cagcaccagc gcctggccgg aagtcggcac
     6961 cagcaaaccg cacatcatct taaaggtggt cgatttaccc gcgccgtttg gccccagcaa
     7021 accaaaaatc tccccacgtt taacggcaaa gttgacgtga tcggtggcgg caaaatcacc
     7081 aaatttcttg gtcagttctt tcgcttcgat caccgtctcg ccgggtgtgc cttctaccgt
     7141 atgcaatatt gcacccagcg gcgattctga ggttccggca ccacccagca aatcaataaa
     7201 cgcatcttca aaacgcggcg tagtttcgtt gatgttgatt tccggcatcc cgtcggcatg
     7261 gcgaatatcg tctggtgtgg cctctttttt gaggatcaga cgtaccgatt tcccctgaat
     7321 catgccgtcg ctgacctgcg gcagttgcaa ggcgcgttgc aacagtttgc ggttgccctc
     7381 gtgtggactg gtcatcagaa agctgcgtcc ggccatggtt tgtgtcaggg cttttggttc
     7441 tccctgatac agcaactcgc cttcgttcat cagtaacacg tcacggcact gctcggcttc
     7501 gtcgagatac gaggtactcc agaggattaa catcccttcg cccgccagct catgcaccat
     7561 ctgccacagt tcgcgccgtg agatagggtc aacgccgacg ccgggttcat cgagcagcaa
     7621 cactttcggt tcgcccacca gggtacaggc caggccgagt ttttgtttca tcccaccgga
     7681 gagcttgccc gccaggcgtc cggtaaacgg cccaagagac gtaaactcca gcaggcgagc
     7741 aaaagtttgc ttacgtgcct cgccggtgac gctgcgcaaa tccgcgtaca gattgaggtt
     7801 ctccatcacc gtgagatctt catacagacc aaatttctgc ggcatatagc cgagcacggc
     7861 gtgcagcgcg ccgtcgtttt tgatcggatc aaagccaatc accgtggcac tgccgctgtc
     7921 gggtttcagt aatcccgcca acatccgcat cagcgtggtt ttacctgcac cgtccggccc
     7981 caccaacccc gtcacataac cggcgtgaat ggtacaatcg agcggcgcga cggcgggctt
     8041 gtccatgccc ggaaagcgtt tttccaggcc gttcagcgtg ataacggcat cattcatgtc
     8101 ctgcctcgtc accgaattgt accgtcactg gcattccctg gcgtaacgca tcatcggcgt
     8161 cggtcaccac aatacgcagg cgatagacga ggtcggtacg cagatccggc gtttcgacgg
     8221 ttttcggggt aaattcagca gtcggcgaaa cgaaaccaat ctgcccgtga tacggcttgt
     8281 ccgggcgacc atcggtataa agcagcactt tgcgccccgg ctgggcctgg tcaagattac
     8341 gttcatcaac ataagcgcgc acccacaccg gacgcgttag tgaaacggta aacaccgtgc
     8401 caccttcatt gaggaccgtg cctggctcca ccgcgcgcgt taacagcgtg ccatcagacg
     8461 gggctatcaa cgttgagtcc tgtaaattca actccgcctg cgccagttgc gcctgcgcct
     8521 gttcgaggct ggctttcgcc tgagcgatgt cctgttcacg gttaccggaa cggtactgac
     8581 gcaatttatc ctgtgctgat ttcagcgttg cctgcgcctg gtcgcgcgag gagcgggcat
     8641 tttccaggtc atttgccgaa atagtgcggc ttttccacaa cccttgctgg cggttataga
     8701 agttctgcgc atagtcatag gcggcttgcg cctgtttcac cgctgcggcg gcctgagcga
     8761 tttcttcatc gcgatacccg gcaagcatca ggtcatactg cgcctgtgcc accgaaacac
     8821 ccgctttcgc ctgcatcagg gcaatctcat acggcttgtg atccagttcg cccagcacct
     8881 ggcccgcttt gatagcatca ccttcgtcca ccgccagcga ttcaacgcgc cccccaacac
     8941 ggaaactaag atttaccgta cgaatatcca cgttgccata cagcgtcagg ccgttatcct
     9001 ggcggctttg ataccaccag tagcctccgg caaccacggc ggcaagtacc actaccgcca
     9061 atccgatcac gacaggtttt ttcatcacta caaactcctt tgcgataatc cttgcaaaat
     9121 caggtcgata tgacaggtca ccgtctggtt gatcagttcg gtcttttctt catcgaacgc
     9181 ggtccagccg gtacgtaaca gaattgtttc tttgccaaga cggaacgcca gaatctcacc
     9241 aatcaacgca tgggtatgaa ggatcatgcg ggtgtcattg gcgtcgcagc cggtccaggc
     9301 ggcaatcagg cgtgtcaggt ggctgtgtag cggactaatc acctgttcgt gcaccaggtg
     9361 gtaggctgcc gtgggagaga gctgctcacg ggagataaac ttgctgaggt tgacggtgtc
     9421 atcctgggtg agcagcttaa tcatgttcct gcaggcgcga aggatcagtt cacggatggc
     9481 agcccgatca ggctgtggtt gcgcgaacaa gcgttcggct tcctcggcat gcggacggaa
     9541 ctgctcgcca ataaaatcgg caatccactg ggcgcaggcg aggtacaaat cttctttcga
     9601 accgaagtag taggtgatgg cagcgatatt ctgcccggcc tgggcggcta tctcgcgagt
     9661 ggtggcgttc attccatatt caccaaactg cgccagtgcg gcagcaatca gctgtttttt
     9721 cgcctgttca cccttgattg tcatggcagg attattcatc gcacagtcca ttcttaatca
     9781 aatgattgat taagattatg actccatagg ggagttgtcc agtatggcta agaattttag
     9841 caacgccagt cacagggata atttatgcgc tgcgtcacaa aaactgctac actccgctcc
     9901 ctcatgacat tgtggttttt gtcattttcc ttttcagtat ctccctgaaa actacaccgg
     9961 taacggtcgg ggcggttcgg agtagttatg tctttcgatt ctttgggttt aagccctgat
    10021 atcctgcgcg ccgttgccga gcagggttac cgtgaaccca cccctattca gcagcaggcg
    10081 atccctgcgg tgctggaagg ccgcgacctg atggctagcg cccagaccgg caccggcaaa
    10141 acagcgggct ttacgctgcc gctgttgcaa cacctgatca ctcgccagcc gcacgccaaa
    10201 gggcgtcgtc cggtacgtgc gctcattctt accccgaccc gtgaactggc ggcgcagatt
    10261 ggcgaaaacg tccgtgatta cagcaaatac ctgaacattc gttcgctggt ggtgtttggt
    10321 ggtgtgagta ttaacccgca gatgatgaaa ctgcgtggcg gcgttgatgt gctggtggca
    10381 accccgggac gtttgctgga cctggaacat cagaatgcag tgaagctgga tcaggttgaa
    10441 atcctcgtcc tcgatgaagc tgaccgcatg ctcgacatgg gctttatcca cgatatccgt
    10501 cgcgtgttaa caaaactacc tgcgaagcgc cagaacctgt tattctccgc gaccttctct
    10561 gacgatatta aagccctggc ggaaaaactg ttgcacaacc cgctggaaat cgaagtggca
    10621 cgccgcaata ccgcgtctga tcaggtgacc cagcacgttc actttgtcga taagaaacgc
    10681 aaacgcgaat tgctgtcgca catgattggg aaagggaact ggcagcaggt gctggtgttt
    10741 acccgtacca aacacggcgc taaccatctg gctgaacagc tcaataaaga tggcatccgt
    10801 agtgcggcga tccacggcaa taaatcgcaa ggtgcgcgta ctcgtgcgct ggctgatttt
    10861 aaatcgggcg atattcgtgt actggtggca actgacatcg ctgcgcgcgg cctggatatt
    10921 gaagagctgc cgcacgtcgt caactatgaa ctgccaaacg tacctgaaga ttatgtccac
    10981 cgtatcgggc gtaccggtcg tgcggctgct accggtgaag cgttgtcgct ggtgtgtgtt
    11041 gatgaacaca aactgctgcg tgatatcgaa aaactgctga aaaaagagat cccgcgcatt
    11101 gcgattccgg gctatgagcc ggacccgtca atcaaagccg aaccgatcca gaacggtcgc
    11161 cagcaacgtg gcggcggcgg tcgtgggcaa ggtggtggtc gcggtcaaca gcaaccacgc
    11221 cgtggggaag gtggcgcaaa atctgcaagc gcgaaacctg cagaaaaacc gtctcgccgc
    11281 ctcggcgatg ccaaaccggc aggcgaacaa caacgtcgcc gccgtccgcg taaacctgcc
    11341 gctgcgcagt aatcttttat gccgggctat gcccggcatc aggctgatga acaaacgcaa
    11401 aactgcctga tgcgctacgc ttatcaggcc tacgtgaact ctgcaatata ttgaatttgc
    11461 atgcttttgt aggccggata aggcgtttac gccgcatccg gcatttcaca acaagcactt
    11521 gtcagcaatt tgagaacacg ggaaaaaatt tatctgtttt accgcccata agccaccgag
    11581 acgttacttc tctatagcca attgttcgcg caactccatt aaaaggtagc ccagtcgatt
    11641 cttgccctta ccatgaccac cgtctcccca gtaagcatcg ttttccgtat gctcaaccag
    11701 ttttgcgggc gcggttgcca gcaagagcgc acgcagttct gcatgctgtt cgaatttagc
    11761 gcgaagtgct tttcgcatca cctgttcttt gaccgactcc cagtttttac gcagaggctt
    11821 agaacgatcg cgccccatgc gtgcggcgac cataggggaa gaaacccgac ggatctcttc
    11881 gcggtatttt tcatcaagga atttttgtgc ctgaaaatag tgttctgagg tgggccaggt
    11941 tttcccgtca accttgatgg gccaggcggc aaagttagaa aaatcaccgt agtcatcgct
    12001 ggtgctgtag aaatttatga tggtgtcttg catgacgtgt tggatccttt gtgctcgaac
    12061 gggcattaaa ccgcattatg ttggtggtta ttgcgagccg ctttccagaa acagaaaaac
    12121 cattacccct gaaaaccgaa aaatgccaca atattggctg tttatacagt atttcaggtt
    12181 ttctcatggc attaaccgcc gcgcttaaag cgcaaattgc cgcctggtat aaggcgcttc
    12241 aggaacagat ccccgacttt attccccgtg cgccgcagcg gcagatgatt gcggacgtcg
    12301 ccaaaacgct ggccggagaa gaagggcgac atctggcgat tgaagccccc accggcgttg
    12361 ggaaaacgct ctcttatttg attcccggca tcgccattgc ccgcgaagag caaaaaacgc
    12421 tggtggtgag taccgccaac gtggcattgc aggatcagat ttacagcaaa gatttaccgc
    12481 tgctgaaaaa gatcattccc gatcttaaat tcactgccgc ttttgggcgt gggcgctacg
    12541 tttgtccgcg taatctgacg gcgctcgcca gtactgtacc cacgcaacag gatctgctgg
    12601 cgtttcttga cgacgaactg acgccgaaca atcaggaaga gcaaaaacgt tgtgcgaagc
    12661 tgaagggcga tctcgatact tataaatggg atggtctgcg cgatcatact gatatcgcta
    12721 tagatgacga tctctggcgt cgtttaagta ccgacaaagc cagctgcctc aaccgcaatt
    12781 gttactacta tcgtgaatgc ccgttttttg tcgctcgtcg ggagattcag gaagcggaag
    12841 tggtggtggc aaaccatgcg ctggtgatgg cggcgatgga aagcgaagcg gtattgcctg
    12901 acccgaaaaa tttactgctg gtgctggacg aaggccatca cctgccggat gtggcgcggg
    12961 atgcgcttga gatgagcgcc gaaatcaccg cgccgtggta tcggctacag ctggacttgt
    13021 tcacgaaact ggtcgctacc tgcatggagc agtttcgccc gaagaccatc ccaccgctgg
    13081 cgatccctga acgtttgaat gcgcattgtg aagagttgta tgagcttatc gcctcgttaa
    13141 acaacattct caatctctac atgcctgccg ggcaggaggc agagcaccgt tttgcgatgg
    13201 gcgaactgcc agatgaagtg ctggagatct gccagcggct ggcaaaactc accgagatgc
    13261 tgcgtggcct ggcggagtta tttcttaacg atttaagtga gaaaaccggc agccatgaca
    13321 ttgtacgtct gcatcggttg attttgcaga tgaaccgcgc gttggggatg ttcgaggcgc
    13381 aaagcaaact ctggcggctg gcttcgctgg cgcaatcttc cggtgcaccg gtgaccaaat
    13441 gggcgacgcg ggaagagcgc gaagggcagc tacacctctg gtttcactgc gtgggaatac
    13501 gtgtcagcga tcagctggaa aggctgctgt ggcgcagtat tccgcacatt attgtcacct
    13561 ccgcaacctt gcgttcgctg aacagttttt cgcgtttgca ggagatgagt ggtctgaaag
    13621 agaaagcggg cgaccgtttt gtggcgctgg attccccctt taaccactgc gaacagggca
    13681 aaattgttat tccccggatg cgcgttgagc cttccatcga caacgaagag cagcatattg
    13741 ccgaaatggc ggcctttttc cgtaagcagg tggagagcaa aaaacatctc ggtatgttgg
    13801 tactgtttgc cagcggacgg gcgatgcagc gctttctcga ctatgtgacg gatttacgtc
    13861 tgatgttgct ggttcagggc gatcagccgc gttaccgttt agttgaactg caccgcaaac
    13921 gcgtcgccaa cggtgagcgc agcgtgctgg tgggcttaca gtcatttgcc gaagggcttg
    13981 atttgaaagg tgatctgctc agccaggtgc atatccacaa aatcgctttt ccgcccatcg
    14041 acagcccggt ggtgatcacc gaaggggaat ggctgaaaag cctcaaccgc tatccgtttg
    14101 aggtgcaaag cctgccgagc gcctcgttta acctgattca gcaggttggg cgactgattc
    14161 gaagccacgg ttgctggggc gaagtggtta tctacgacaa acgcttgctg accaaaaatt
    14221 atggcaagcg actactggat gcattaccgg tatttccgat agagcaaccg gaagtccctg
    14281 aaggtatagt taaaaagaaa gaaaaaacga aatccccacg ccgtcggcgg cgttaatgat
    14341 gtgagtcagg taaggagtcg taaatggact atcgcaaaat cattaaagag atcgggcgcg
    14401 ggaaaaacca cgcgcgcgat ttagaccggg atactgcgcg cggtctgtat gctcatatgc
    14461 tcaatggtga agtccctgac cttgagttgg gcggcgtatt gattgcgctg cgtatcaaag
    14521 gagaagggga agcagagatg ctcggctttt acgaagccat gcaaaatcac accatcaagc
    14581 tgacgccgcc agcaggcaag ccgatgccga ttgtcatccc cagttacaac ggcgcgcgta
    14641 aacaggccaa cctgacgccg ttgttggcga ttctcctgca caaactcggt tttcctgtgg
    14701 tggttcacgg ggttagcgaa gatccaaccc gcgtgctgac tgaaaccatt tttgaattga
    14761 tgggtattac gccaacgttg cacggcgggc aggcgcaggc gaagctcgac gaacatcaac
    14821 cggtgtttat gccagtcggc gcgttttgcc cgccgctgga aaaacaactg gcgatgcgct
    14881 ggcggatggg cgtgcgtaac agtgcacata ccctggcgaa actggcgacg ccatttgccg
    14941 aaggtgaggc gctgcgtctt tccagcgttt cgcatccgga atacattgga cgcgtcgcga
    15001 agttctttag cgatatcggc gggcgggcgc tactaatgca tggtactgaa ggtgaagtgt
    15061 atgctaatcc gcagcgctgc ccgcagatca atctcattga ccgtgaaggg atgcgggtgc
    15121 tgtatgaaaa acaggacact gctggtagcg agttactgcc acaagcaaaa gatccggaaa
    15181 ccacggcgca gtggattgag cgttgccttg ctggcagcga accgattccc gaatcgctga
    15241 aaatccagat ggcttgctgc ctggtggcta cgggtgaagc ggcaactatc agcgacggcc
    15301 tggcgcgcgt taatcaggca ttttaattct tttctcagcc ggatgagcca tgtcatccgg
    15361 cttttacccc accgcaatat gaaattcctg catctttatt gaccttccca cgcccggcgt
    15421 gcagcataaa aatacaacaa acacataaca taaacaggag ttaaccatgg aaagtggtca
    15481 tcgctttgat gctcagacgc tgcacagttt tattcaggct gtatttcgtc agatgggtag
    15541 cgaggaacaa gaagcgaaat tagttgccga tcatttaatc gcggcaaacc tggcagggca
    15601 tgattcacat ggtattggca tgatcccaag ctatgtacgc tcctggagtc aggggcacct
    15661 gcaaattaac catcatgcca aaaccgttaa agaggcgggg gcggcggtca cgctcgatgg
    15721 cgatcgcgca tttggtcagg tcgcggcaca tgaagcgatg gcgctgggga ttgagaaagc
    15781 gcatcagcac ggtattgccg ccgtggcgct acataactcg catcatatcg gccgtatcgg
    15841 ttactgggcg gagcagtgtg cagcggcggg gtttgtctct atccactttg ttagcgtggt
    15901 cggtattcca atggtcgcgc cgttccacgg tcgcgacagc cgctttggca ccaatccgtt
    15961 ctgtgtggtt ttccctcgta aagataattt cccgctgttg cttgattacg ccaccagcgc
    16021 cattgcattt ggcaaaaccc gcgtcgcctg gcataaaggc gtccccgtgc cgccaggttg
    16081 cctgattgac gttaacggcg tgccgacgac caatccggcg gtaatgcagg agtcgccgtt
    16141 gggttcgctg ttgacctttg ccgaacataa aggctacgcc cttgcagcga tgtgtgaaat
    16201 tcttggcggg gcgctttccg gcggtaaaac gacgcatcag gaaacgttac aaaccagtcc
    16261 cgatgccatt cttaactgca tgaccactat catcatcaac ccggaactct tcggcgcgcc
    16321 ggattgtaac gcgcagaccg aagcctttgc cgagtgggtg aaagcctcgc cgcatgatga
    16381 tgataagccg attttgctac cgggcgagtg ggaagtgaac acgcgtcgcg aacggcagaa
    16441 gcaggggatt ccactggatg cgggaagctg gcaggccatt tgtgatgcag cgcggcagat
    16501 tggtatgccg gaagagacgt tgcaggcttt ctgtcagcag ttagccagct aaaaaaaagc
    16561 ccgtccagtg gcggacgggc aaacaagggt aacataggat caatgagggt tagagcatat
    16621 gcgtctgtcg gcaaacagac agggaaatac ttgtgctgga cgtagcgtaa acgcctgatc
    16681 cgtccaacgg ttcggatttt gtaggcttga taagacgcgg tagcgtcgca tcaggcatct
    16741 gttgccggat gcggcgtaaa cgccttatcc gtcctacgaa ttacttataa attaccgcag
    16801 taccgcttaa tttattgttg ttggtggcgg aagtgatgct gtatccactg gcaccggctg
    16861 ccgcggcttt ctcagccagt ttcgcttcca gggcatcgag ggtggatgcg ccatcggcag
    16921 aaaccacgcc gattttattc atgttctgtg cctgggatgc cgttaccggt tccgccgcga
    16981 acacgccaaa tgacagagtt gaaagagcca tagcagcaac aacagtattg atagttttca
    17041 taattaatct ctcgcaggtg atttttgtat aagtgacgtt gtttcgtcga tgtgatgagt
    17101 atcacgtttt tttacgagag ataaaatcga agagaattga ccgccttgtt caaataaatt
    17161 gattgatatc taacctactg ataaaccatt tatttgttgt acttatgaac ttgttttata
    17221 ccgcgtggca gtcacagcag cgttaaaggc acggaatgac acgcaaaata caaattatac
    17281 tcgctatgca tctcgccagt tgtagttaac gtaactggct gtctttcgaa cctctgcgat
    17341 tatatcctgt ataagctggt ttttgtaaat ctttctcctg ctgacaatga atacataagc
    17401 gcacgccagg aatggcttcc cgacgggcct gcgggatggg ggcaccgcac tcttcacatt
    17461 catccaggct ttcgccgcgc ggaatttcac cccgagcgcg ggcaatcgca tcttcaattg
    17521 tactgttgat ctgttcgttg acggcgtcat cgttagccca accggatgcc atatcgacct
    17581 ccccatatca atacttgtac agttaagtgt agctaatcca gggacgaact cgggcagttc
    17641 aagcatcaga tctccgacca ttcccgcagc agattatgat aaagattaag cagcgacagg
    17701 atctcttcac tttcgccgta gcggcttttc agcgactgaa tattgttgtc cagttcaaac
    17761 agcatggcgc gctttttatc atcgcggatc atcgactgga tccacataaa tgatgccact
    17821 cgtacgccgc gggttacggg tgtcacgcaa tgcaggctgc tggagggata caacacgaga
    17881 tcgcctgccg ggagttttac ccgatgttgt ccgaaggtgt cattaacgac cagttcgccg
    17941 ccgtcgtagc tttgtggatc gcttaaaaac agcgtggcag aaaggtcagt acgcatccag
    18001 ccgttttgtg gatggctgcg tactgcgcca tccacatgga aaccataggt ttcattgttc
    18061 tgatagcgat taaacagcgg cgtggaaagg gtacgcggca aggccgcggc aaagaataaa
    18121 gcatgttggt taaccgcgtt cagcacctca ttttgcaggg cggcgtataa cgtgctgcgg
    18181 gtgtcgacct gttgattgtt cttaacttgc gcaccttgtg cgccggtggt gacgcgtcca
    18241 tccacccatt cggcttgttc cagttgttcg cgaaaacgag cgacgtcctg tggcgataac
    18301 acgccgggaa tgtggtacat catacaattt ctccaaaaag tggggcctgc gccccacatc
    18361 tgattcagaa atgcatattg gctgtgagca agaaggttct tggctcgccc gggtgataac
    18421 ggtagccgct cttgttgatt gaggcgacgt aatcggtatc aaacaggttg taaacgttta
    18481 gctggaagtc gagattgcga ttaactcgat accccagttt ggcatcggcg acccagtaac
    18541 cttcggtaaa cgctggcgtt cccaccgcgc cgtctgaacc tttatgcata ctgccgatat
    18601 agcgtgcgcc cgcgccaaca gagatatcgt cggttgcctg atattggctc cataaggtga
    18661 aggcgtgctc cggggtatac ggcagcgatg aggaaccatc ctgggcaaca tctttgccgt
    18721 ttttgatggt tgctttttgc tgggtatagc cgccaatcac ctgccacgcg ggagtgatat
    18781 tcccggccac ggatatctca tagccttcga cgcgtttctt accgtattgc gagtaagttc
    18841 cgtcatcatt ttgctcaact tcattttcga tatcagtgcg gaacagcgcg gcggtgagca
    18901 acagacgttt atccagaacc tgccatttgg tgccaatctc gctggtgttg gctttttgcg
    18961 gtttaaaatc ggtgcggttg gcactgttac cgctgccaga ctgcgcaagg gcgaagttgt
    19021 tgccgcccgg aggctgctgg gaaacggcat agttaatata gacattgccg ttttccgtca
    19081 ggtgatacag cgccccggct ttccagttca tcagattgcc cgacttggcg gtgtcgacgg
    19141 tggtgaccgg agaacctttt gccacaccag ttgggcaggt gatggcaccg cgtccgctgc
    19201 cgccgcaggc ggtggcactg tcatattcag tatgataatt atccagacgg atcccgccgt
    19261 tcagctcaaa atcacgggtg atttgcagcg tatcaaaggc gtaaattgcg aaggtatccg
    19321 tctgaccatt ggcgtttgcg ccgttgcgcg tcaggccgcc gggatgaatg ctgctgtcag
    19381 gatgataaat atttaccgcg ggtaacgtca ccggattaac gccgtagtta gtctgcgttt
    19441 cacgggtaaa ttccacgccg gtactgacat catgaccgat agaaccggta tagaacgtcg
    19501 aggtcaggtt ggtctggttg gtgagaattt tattactcac atctttggta ttcgccgtgc
    19561 gtgaccaggt ccagctattc acatcgctgg tgggctgagt aatattcgac gccccgccca
    19621 taatcgccgt catcaggtaa tcctgcttta cgcgcgacca acgggtagta ttgcgaatgg
    19681 tggtgttatc gttgatgtcg tgctcaaaac gcatggtggc ggtgtcggtg gtcgaatcgt
    19741 cgtaatcgga atccgtgccg taaaagttat gagtatcaac ttttccggaa tgattcaggg
    19801 ccgccgttcc cgcagatggg gcagaatagc ccggcaaacc gatggtcgga atgccgccgt
    19861 ctggcgtgtt gtgctgggtg acatgcagat aattaagata caaacgattc gctgtaccaa
    19921 ggccaaaagc gacagaaggg gcgacgccgt aacgctcatt tttgactttg tcgcgtccgg
    19981 catcgtgcgt tttttcgccc attacattca ggcgcaccgc ggtggtatca ccaatgacct
    20041 gattgacgtc cagcgtgccg cggcggaacc aggcgctgcc aatactggcg gaggcgtcaa
    20101 tgccggaatc attgcgcggc tgcttgctga tcatattgat cgagcctgtc ggtgcgctgc
    20161 gcccgtagtc ggtgccggac ggccctttaa tcacttcgac ctgctcggta ttgaaggtgt
    20221 cgcgcgagac gctgccgata tcgcgaatgc catcaatata aatactgtta gagatatcgg
    20281 caccacgcat ataaatggcg tcgccagtgg tggagttacc gttctcaccc gcaaaaaacg
    20341 cacccacgcc ggggacgttt ttgagcgcgt cggtaaggtt ggttgcgccc tgatctttaa
    20401 tcacttgttc agaaattacc gtcatcgtgc gggtagtatc cgctaccgga cgcgagaatt
    20461 tcggatcggc agattgttgt ggcgcataaa gcgaaggcgt tgatgcttcg acaaccagcg
    20521 tgtcatccgc gttagtttgc ccttcggcgg cgagtgcctg agccacaggc gtgatgccaa
    20581 tacaaagacc ggcaaagaac gtgagcgaat gaaattgtct ggcagggaaa ttgcgattgt
    20641 tttccatttt gcaggtgact ttttcttata tgttgaaatt ttcactgcca cgagaaagca
    20701 tctctgcgga tgcgatgcca ctgttaatcc cgtgatagtg ttcgtgcaag tgataataat
    20761 tttgataagc attatcactt ggagcgattt tctatcaccc attgcgcgaa aaagaaatac
    20821 atttatttac attgagttca tatttgatgt tttttgatta caaaacagac gaaaactgca
    20881 tccagaggcg aatgcagtta cgttggtggg gttatttgta gatggttgca gtaccgaaca
    20941 tctggtcatt tccgcctgcg gaattaatga cataaccttt cgcgccttgt tcgcgcgctt
    21001 tttctgccag tttatcctcc agatcgctta ggtttgatgc gccagttgcc gaaacggtac
    21061 cggcgggccg tagctgaccg ctgtccaggt tactcatcgg ttgcgcggca taagccgtga
    21121 gagagattcc gctcaggacg gtggcaatca gtagtgtgag gcactttttc ataatgacat
    21181 cctcataaat acaacgggta gtgcctgata agtgtaggtc gcctgcgcga tgcaaatgac
    21241 gcaaaaaatt gacagagatt tgcttatttt ttgaacgata tatttttaca aaataagaca
    21301 aatcaatgac atgcataatg ctgattttta tgatgaattg ccataaacca tagccatgtt
    21361 aaggttttgg ggaatgataa acgcagccag atatttaccc tgtcgcgtta tgcgggtatc
    21421 ttacgccgct gcttaaagga gaatgctatg tccgcccaga aaccggggtt gcatccgcgc
    21481 aaccgtcatc acagccgcta cgatctcgcc acgctttgtc aggtcaatcc tgaactcagg
    21541 caattcctca cgcttacacc cgccggggag caaagcgtag actttgccaa tccgctggcg
    21601 gtgaaggcgc tcaataaggc gttgctggcc catttttacg ccgtagcgaa ctgggatatc
    21661 cccgacggtt ttctctgccc accagtaccg ggccgggcgg attatattca tcaccttgcc
    21721 gatttactgg cagaagcaag cggaacaatt ccagcgaatg ccagcattct ggatatcggc
    21781 gttggtgcga actgtattta tccgctgatt ggcgtacatg aatatggctg gcgttttacc
    21841 ggtagtgaaa ccagcagcca ggcgttaagc agtgcgcagg cgattatcag ttctaatccg
    21901 gggcttaacc gcgccattcg tctgcgtcgg caaaaagaga gtggggcgat ttttaacggc
    21961 atcatccata aaaacgagca atacgacgcg accttgtgta acccgccatt ccacgattcc
    22021 gccgctgcgg cacgggcagg tagtgagcgt aaacgccgta acctggggct gaacaaagac
    22081 gatgcactga actttggcgg ccagcaacag gagttgtggt gtgaaggcgg tgaagtcacc
    22141 tttatcaaaa agatgattga agagagcaaa ggcttcgcga agcaggtgat gtggtttaca
    22201 tcactggtat ctcgtggtga aaacttaccg ccgttgtatc gtgccctgac ggacgtgggc
    22261 gcggtgaagg tggttaaaaa agagatggcc caggggcaaa agcagagtcg ctttattgcc
    22321 tggaccttta tgaacgacga gcagcgccgc cgttttgtca atcgccagcg ttaaagcgtt
    22381 ggttcccccg gcggtaacgg ttcagccggg gtcgcgcccg gagcaggcag cacctgataa
    22441 gtctgcactg gcgcgcgaac gcccgccagg tcgaaatgtt ttttcacctg gctgtcgagg
    22501 gcaaagcgta ccctccactg tttgagtggc agcgtggtga acgaaacacg cagtgtaaac
    22561 gcggtattgc ttaagccgac aatcccggca aaattcggtt caccaataat cagcccgcga
    22621 atttcttcgt tttccattaa ttccgctacc gcatctttca gtgcctgatt ggctttatca
    22681 gcatcttcat ggcgatcaac atcataattt gccaccaccg aaccaatgcc gcggacaaag
    22741 ttagcaaagg tggttatcga agaccacgga atgatgtgat acgccccggt atcctgtcgt
    22801 acgcccacgg agcgaatcga catccgttcc actgtgccgg tcaacggccc gatagtcacc
    22861 aaatctccag tgttcatgcc gttttcaaac tgaataaata ccccggtgat aatatctttc
    22921 accagcgttt gcgaaccaaa cgagatagcc agacctaatg ccccggcacc tgccagcaat
    22981 ggcgcgatat tgacgccgat ttccgacaac acaatcatga tggtgatggt actgataatc
    23041 accgccagcg cgttacgaaa cagcgtaagc agggtacgcg tgcgggcgct gggtagcggg
    23101 cggccatgaa tatccgaagc cagccggttt tcgatcaaac tggcgagcac cgtccagcca
    23161 accgccgaga agaaaagaat gagtgcgata cggatcagga tatctacggt tttctgcccc
    23221 gcgccgtttt gcagccagtt ccagaaatcg aacaatcccc atgcgctcaa cagcaacatt
    23281 accgccacgc agactgtcag aatacgcgcc gttttcagcg ccgccgacag ccagccattc
    23341 aaccgttttt gcagctccgg atagttacgc tgagtatgtg gcgagagagt gatggttttc
    23401 gccagccagc gggaaaacat accggaaaca aacgctgcga taccaataat cgccaggctg
    23461 cgcaccgttg cacccatcat aaatttcagg ctgttgcccg gatcgaacaa cgaaaagaaa
    23521 aacagcacga taaaataggc gcttgccagc cagtgccaca ccagcgcaaa ggcgcggata
    23581 aacaggctga aaaaggccag cgaatgctcc gcgaagttga gcaaatgctg ggtaatctct
    23641 tttttattac gaaagatcag gtacaacgcc cagacggtca tgcacagcat aatgatgacg
    23701 ttcgccagcg cacctatctg tacattcacc tgattagaga taatcggcac ggccacaatc
    23761 aggccataac ctatcaaact gcttaaccag ctcaggcgac gactccagta acgggcgctc
    23821 tcgtcctgaa tcgtgaacgg gcgcagctcc gccacgtttg ggcaaaaaat caggcgtagt
    23881 acggctttga aaaattcaat gagggcaaag gcgttgagaa acaaactttg ttggaaagcg
    23941 atggtgcgac tgcctgcatt caggttgtcg cttaatacct ggccgacaaa taatgtcagg
    24001 gccagtaaca gcaggtcgat aataaacgcc ccgataatca tcgccggaag ctgcaaccag
    24061 ttgctgcgct cacgattttt ttgccgcgcc cactgaccca ttttgcgata cagcggcagt
    24121 gcgcacaggc gaatcagcca gtaaaaacca aacactaata ccgctaacat tgaaaaatgg
    24181 gtcagcgcat tgctgaaggt ttgtggatta aacggcttat gcggggagcc ggtgatattg
    24241 cgataaagtt gcccgaagcg ggcggaaagg gcttcaccat aatggcggct gacttcggtg
    24301 accttttgca gcacggtttg ctcttcgacc agcgtcggcg gcactatttt tggtaccggt
    24361 tcagcagggg gcgtagcggc aacggtgcgc aactggtcga tcaactcttt acgcgaggtg
    24421 tcattatcca gcacatccgc cagtgcgcca taggccgctt ttttttgttc gatatccggt
    24481 tccggggccg gttcagtcgt tgagtccgtt gtcgttgtgg ttgtaacgcc gggtatggat
    24541 accgcgtggg caggtgcgcc cagcaggcag aagaggatga acaggatcca ccgcatgact
    24601 cctccagtga gaaaatagag caaaaagata agtatagatg ctggaggagg ggcgatttta
    24661 aatgagagga atctggtgtg cctccctttc gggtgaaagg gaggaaggga tattaagcag
    24721 gttgctgaca acgtgcgggc ttttttgccg gatgcggcgt gaacgcctta tccgtcctac
    24781 aagttcatgc aattttaata agttgccaga tatcctgtag gcctgataag cgtagcgcat
    24841 caggcaatgt gtcttattaa gagacgtgct gcaaaaattc ctgcaagcgc tggctcggcg
    24901 ggttcttgat caacacctgc ggattgccat cttccgcaat ccggcctttg tcgataaaga
    24961 tcagccgcga agctactttc tcggcaaaac cgatttcgtg ggtcacgatc accatcgtca
    25021 tcccttcttc agccagatcc tgcataacct tcagcacttc atggcgcagt tccgggtcaa
    25081 gagcggaagt cggttcatca aacagcatca ttttcggctt caccgccagc gcgcgggcaa
    25141 tcgccacacg ctgctgttga ccaccagaaa gttcggaagg gtagtgatgt gcacgttctg
    25201 ccagaccgac tttcgccagc agctcacgtg ccagtttttc cgcctcttct ttgttcgcgc
    25261 cacgcacgcg tagcgggcca aacatgacgt tttccagcgc cgtcagatgc gggaagaggt
    25321 aaaactgctg gaacaccata cctgcttcct ggcgaatcag gcgctcgtca actttcggat
    25381 cgttaacctt caggccatcg acaatcagat cgccggaggt gatttcttcc agtttgttga
    25441 tgcagcgcag cagggtcgat ttaccggaac cggacggccc gataatcacc acgacttcgc
    25501 cctgggcaat gttcaaatcg atattgtgca gcacctgggt tgggccaaag tgcttggaga
    25561 cgtttttaaa ttcaatcaca ggattttcat ccttctttcc agacgacgca gaataaagct
    25621 cagcaccagg gtaataatca gatagaacac cgccacggcg ctccagatct caagggcgcg
    25681 gaagttaccg gcaataattt cttgcccctg acgggtcagt tccgccacgc cgatcacaat
    25741 aaacagcgag gtgtctttaa tgctgatgat ccactggtta cccagcggcg gcagcatacg
    25801 acgcagtgcc agcggtaaaa tgacgtagcg aatggtttcc caacgtgaaa gaccgagcgc
    25861 cagtcctgct tcacgaaaac ctttgtggat agacagcacc gcaccacgcg tgatttccgc
    25921 aatatacgcg ccggagttga tcatgatggt gaccaccgcc gcagtaaatg ggtcgatgcg
    25981 taagtcgtta aacgccatcg gcagggcgaa ataaataaac atcacctgga cgacgatagg
    26041 tgtgccgcgg atcacttcaa taaagaccag cgcgacgtgg ttggctatcc aacctccgaa
    26101 ggtgcgtgca aaacctgcca gcaatccgat taccagaccg cctgccagac cgaggaccga
    26161 aatccacagg gtcattttgg caccttcaat cagaagcgga atggcaggcc agatggcact
    26221 ccagtcaaac tgcatatgtt gttcctgtta ccgtggtgta aataatcaaa ttcagggtaa
    26281 ttaatggccc cggagcgggt ttgcgcccct caccctaatc ctctccccat aggggagagg
    26341 gaactgccag tgcgttttac aggtgtagcg ttattatttc ggttcagtac cgaaccattt
    26401 tttgtagatt tcgttgtaag ttccgttctc gcgcagggtt ttcaacgcgc cgttgacttt
    26461 gtcacgcagc tcgtcgctac ctttcgggaa cgcaataccg tattgctgcg cttccagaga
    26521 gtcacctacc gctttgaact gaccgttacc ggcggttttg atgaagtaca gaatgtttgg
    26581 cgtatcgtgc agaacggcgt ctgcgcggtt ggtgcccagt tccatatagg cgttatcgat
    26641 gttcgggaac tgacgcagat ctttagtttt gatgtttgct ttcgcgtaat caacggagcc
    26701 agtaccgctc ttcacagcaa ccactttccc gtcgagatct ttcacgcttt tcacatcgtt
    26761 attgttagct ttcaccatca ctaacaggcc gcttttgtag tagccgtcag agaaatcgat
    26821 cgctttttta cgctcgtcgg tgatggtaat gcccgccagc gccagatcga cgtttttggt
    26881 ttgcagtgcc ggaatgatcc cactgaaatc catcggcttc agttcgtaat ccagcttcag
    26941 ctctttagcg atggcagccc acagatcaac gtcaaagccc acatatttat cgccctgttt
    27001 aaattcaaac ggaacgaagg cggtatccgt cgcgacaact aattttttat ccgcggcatg
    27061 agaagaaacc gcaaaagcca gggtcagtgc agccagtgaa acttttaata cagacttcat
    27121 agcatttcct tttttctatc cacgggacga tcccctgcgt gacattcaca tatatgaaaa
    27181 aatcgtgcca gttttgcaac tccttgattt ttcgagatgc ggcaaaaacg tgatgcacga
    27241 tttatagggc aataccctga agatgcacca ttctggggca ccaatctggt gcgctaaaat
    27301 tgtgcactca acacagtgat tatttagcgt aaaacctgat gaaaaaacaa tctttatgta
    27361 acgattgtgt gatgatgtgg atacaaaaaa tttacttaat cagctggaga tagcagatgg
    27421 atgcactaaa taagtgcgtt gaggtgggct gcaaaacaaa acggcctcct gtcaggaagc
    27481 cgcttttatc gggtactaaa gttctgcacc atcagcgatg gatttattcg atgttagact
    27541 cgataaacca caggaattta tccaggtcgc gagacgcggc ggtcaggata tctgcggtgt
    27601 cgtcatcttt cgcttcgcca atcgctttgc gtacgtcatt agcgacgatt gcgtaacggt
    27661 cagccagttc tttcaggtga tcctgaacgt tgtggatgtc cagcgggtaa cttttcagcg
    27721 gggttttgct gttgataact tgagtggtcc ccagagctac accgcccagc tgcactgcac
    27781 gttctgccat ggtatccaga tgatcgatca gtgcggtgcg gaagccatcc agcatttcat
    27841 gtacggcaat gaagttagcg ccgcgcatgt tccagtgcgc ttgtttggta atcaaagaaa
    27901 gatcaataaa ctggataacc tggcgattca gcaactctac tgttgctttt ttctcgctgt
    27961 cggagacatc gttgcgggta taaagcagat tggtcgcttt tgatttaact aatttagcgg
    28021 tactcataat ttcatatcct cttgatgtta tgtcccagta attaacgaga ctaagtatag
    28081 caccggctat gtgttccgct attctggctg ttcctatcac actaatagtg gtaacaagcg
    28141 tgaaaaa
//
LOCUS       DAPHBJ010000043        28087 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.043, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000043 DAPHBJ010000000
VERSION     DAPHBJ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.043"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..326)
                     /locus_tag="RHZ76_001648"
     CDS             complement(<1..326)
                     /locus_tag="RHZ76_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV2812303.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQRLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQ"
     gene            334..840
                     /gene="tnpA"
                     /locus_tag="RHZ76_001649"
                     /pseudo
     CDS             334..840
                     /gene="tnpA"
                     /locus_tag="RHZ76_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(858..1526)
                     /locus_tag="RHZ76_001650"
     CDS             complement(858..1526)
                     /locus_tag="RHZ76_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HDV2812304.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(1829..2422)
                     /gene="tehB"
                     /locus_tag="RHZ76_001651"
     CDS             complement(1829..2422)
                     /gene="tehB"
                     /locus_tag="RHZ76_001651"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HDV2812305.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVMEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFAGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDSADYPCTVGFPFAFKERELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(2419..3411)
                     /gene="tehA"
                     /locus_tag="RHZ76_001652"
     CDS             complement(2419..3411)
                     /gene="tehA"
                     /locus_tag="RHZ76_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HDV2812306.1"
                     /translation="MQSDRVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFIARLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            3535..4515
                     /locus_tag="RHZ76_001653"
     CDS             3535..4515
                     /locus_tag="RHZ76_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HDV2812307.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(4510..5046)
                     /gene="rimL"
                     /locus_tag="RHZ76_001654"
     CDS             complement(4510..5046)
                     /gene="rimL"
                     /locus_tag="RHZ76_001654"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HDV2812308.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIGS"
     gene            complement(5109..5333)
                     /locus_tag="RHZ76_001655"
     CDS             complement(5109..5333)
                     /locus_tag="RHZ76_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HDV2812309.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(5473..7128)
                     /gene="opgD"
                     /locus_tag="RHZ76_001656"
                     /gene_synonym="mdoD"
     CDS             complement(5473..7128)
                     /gene="opgD"
                     /locus_tag="RHZ76_001656"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HDV2812310.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(7352..8695)
                     /locus_tag="RHZ76_001657"
     CDS             complement(7352..8695)
                     /locus_tag="RHZ76_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV2812311.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     LLLEEYEQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARNHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8912..9835
                     /locus_tag="RHZ76_001658"
     CDS             8912..9835
                     /locus_tag="RHZ76_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2812312.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(9873..11513)
                     /gene="trg"
                     /locus_tag="RHZ76_001659"
     CDS             complement(9873..11513)
                     /gene="trg"
                     /locus_tag="RHZ76_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HDV2812313.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEIDKVTQQNASLVEEASAAALS
                     LEEQAARLTEAVDVFRLHKHSVSAKPRGAGEPVSFATV"
     gene            11912..12061
                     /gene="hokB"
                     /locus_tag="RHZ76_001660"
     CDS             11912..12061
                     /gene="hokB"
                     /locus_tag="RHZ76_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HDV2812314.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(12133..12306)
                     /locus_tag="RHZ76_001661"
     CDS             complement(12133..12306)
                     /locus_tag="RHZ76_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812315.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(12551..13081)
                     /gene="cybB"
                     /locus_tag="RHZ76_001662"
     CDS             complement(12551..13081)
                     /gene="cybB"
                     /locus_tag="RHZ76_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HDV2812316.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            13270..14271
                     /gene="gap"
                     /locus_tag="RHZ76_001663"
     CDS             13270..14271
                     /gene="gap"
                     /locus_tag="RHZ76_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDV2812317.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(14313..15752)
                     /gene="aldA"
                     /locus_tag="RHZ76_001664"
     CDS             complement(14313..15752)
                     /gene="aldA"
                     /locus_tag="RHZ76_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDV2812318.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAEHAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVDGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(15949..16749)
                     /locus_tag="RHZ76_001665"
     CDS             complement(15949..16749)
                     /locus_tag="RHZ76_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDV2812319.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(17021..20923)
                     /gene="hrpA"
                     /locus_tag="RHZ76_001666"
     CDS             complement(17021..20923)
                     /gene="hrpA"
                     /locus_tag="RHZ76_001666"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDV2812320.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESHGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            21124..21729
                     /gene="azoR"
                     /locus_tag="RHZ76_001667"
     CDS             21124..21729
                     /gene="azoR"
                     /locus_tag="RHZ76_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDV2812321.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIISA"
     gene            complement(21783..23088)
                     /locus_tag="RHZ76_001668"
                     /pseudo
     CDS             complement(21783..23088)
                     /locus_tag="RHZ76_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
     gene            complement(23078..24835)
                     /locus_tag="RHZ76_001669"
     CDS             complement(23078..24835)
                     /locus_tag="RHZ76_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HDV2812322.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKSSGPRGYSPSLARSVRDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWIHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRGASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQHLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRALAFEKMQ
                     SFISRLYANKSQKFDYQHEDCTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSSGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(24851..25747)
                     /locus_tag="RHZ76_001670"
     CDS             complement(24851..25747)
                     /locus_tag="RHZ76_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDV2812323.1"
                     /translation="MLEKSLATLFALLILATLINRFLLWRLPERKGGEVTLRIRTWWG
                     IVICFSMVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWGIPLNYLLIGFNC
                     FELFLLFIPLAGFLILATGQVLVGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLMGGVITIMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(25747..26352)
                     /locus_tag="RHZ76_001671"
     CDS             complement(25747..26352)
                     /locus_tag="RHZ76_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDV2812324.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LTFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAEI"
     gene            complement(26523..>28087)
                     /locus_tag="RHZ76_001672"
     CDS             complement(26523..>28087)
                     /locus_tag="RHZ76_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HDV2812325.1"
                     /translation="QELQMLDEGNVAAFEGRDIATFDLAIIRTLPRLKGISANLRKQL
                     INSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDREPLSVEIMTRMSRLPDG
                     VGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPESIIDGYIRSDDSSIRQV
                     GAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRMTPQESDKVAAYLYQKF
                     ENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVIHSDAALQRLLTRFNDP
                     EGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETSADMILSELISHGEVDD
                     QMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPRNDIWAKFFLRQENSSR
                     AQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLTFFKFGALNNRHTPPAV
                     LAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPELDLVRQLALNGKTRAI
                     REQAMRKLDELY"
BASE COUNT         7292 a         7230 c         6491 g         7074 t
ORIGIN      
        1 ttctgtcccc gctttttgaa tcggggaaaa gcagcccgct tccggaagaa gtttttgtaa
       61 gcccgctcaa ggtctttcag tgactgttgc aatggctgtg agggagaatc tttaagcctt
      121 tgcgtttcag tggcattttt ccactcaacc agccaggaag ccattttccc gtaagggatg
      181 tatttattac cggcctcatg attctcattc tgacgtgcca gagcacgatt gaaaacgaaa
      241 cgacatgcgc ctgcgaagcg cctcatttca cactcctgtt gaccacctgg tcttaactgg
      301 aatttaaatg cctgtaatcg tttcacaggt caaatgatac tttggtctgt gaaaaaagaa
      361 accgatatcc ggcgtggaag gcattgtgtt ttcctgatgc atgttcacct ggtctttgtc
      421 accagatacc gacgccagat ttttgaccat gacgcgacag aaaaactacg cacttacttt
      481 tcaaatgtat gtgctgattt tgaagctgaa ctggttgaaa tggatggcga accagatcac
      541 gtccatttgt taattaacta tcctcccaaa ctggcgatat ccagtctggt aaacagcctc
      601 aaaggcgtat cgggtaggtg actgcgacga gatcgaccag atattgcagt caggtattac
      661 tacaaaggcg ttttgtggag tcctggctat tttgccagta gctgcggagg tgcgccaata
      721 tccgtcatcc gccaatacat tgaacaacag caaacacctg gtcaggtgga aaaccgcgcc
      781 ttatatcccc gccctgaagg atggggtttt acggcgcacc ggataaaaag cgccatctaa
      841 cgatggcgcg tctggaacta ctttttgtta acgtcaaaca tggtggcatc ggtcgccatg
      901 tcatcaataa cttgtttaat attttcgaaa gccattggcg tactttcgtt attcaggtct
      961 ttaccttcgc cctgacgaac gactttgata accggtttat tagtcgctgc atcaatcagc
     1021 tcaccttcaa aatagaggcg agtatccatg gtacggtggc ctgtagccat ttgcgtcccc
     1081 gcaaccacta atgcaacagg aaccacttca tagaattgca gcccttcttt gctggtatct
     1141 acaccggtaa tggccccacg gaaaatcaga ctacgcggcc cagcggtggt aaccagtggt
     1201 ttacgctgcg ctatcgcttc tttcatttcg gtgttggtat agttcaaaat tttatccaga
     1261 actttctgcc ctacctgggt cgacggtttc ggtaccggat aataagtgat tgggttccag
     1321 acgatgctgt catatttgct ttgatcaaaa ctcgggtcta cccaacgtaa aacaggttta
     1381 cctgtagccg aggttgtttc ttttaaatca gagtaattgt ttaaaaaacc agaatattta
     1441 tctggctggg tgattttaga ggcacaacca gacagagcca ataagccgca taaagctgca
     1501 actttcgcaa atgatgtggt acgcatgaat agtttcctga gatgattata ttactttcta
     1561 agttatagca aaaagcgccg cttcgtgttg tggcaaaaaa agtgttggtg aaaataaatt
     1621 tttcagcccc ttttcagacg acatagtcaa tgccgaaaaa ggggctaagt caatcatcag
     1681 tagttaaatt tcagtaagtt aagctgctaa aaaacttcga ctactgtgat taatttcaca
     1741 tccctttgat aaaaaatatg cttaccgaaa agaagaacag taaactatct cagcgtgggg
     1801 aattttcacg cgctttgttc ttaccgggtc attttttacg tgccagcatc gtggcgaaac
     1861 gcagtttaat acgattaccg ttggcgtcgg tgcggtgcag ctcgccgacg tcttcattgt
     1921 atttcaccat ctcccagcct tcgtaatatc gacgtaattc tctctctttg aaggcaaacg
     1981 ggaagccgac ggtacatgga taatcagcgc tatccatcgc cgccacaatc aggttgtaac
     2041 cgccaggttt cgtgcaacgt tgcatattgg caatcagccc tgggatggtt ttagcctcaa
     2101 ggaacatcag caccacagtc gaaagaataa aatcgtactg tccagcaaat gtgaggttat
     2161 tcagatcaac gacccgagtg tgtaaattat ccagattttc aatagattta atgcgctcga
     2221 cgttggcgat actcatggca tttttatccc atgcgtcaac atcataacca ttggctgcca
     2281 ggtaaagact gttacgacca ttgccacagc ccagatccag cgttttaccc ggtttaacca
     2341 ctttcaccgc ttccattact tcagagtgtg tgcgggttaa ttcatattta tcagtaaaat
     2401 agttttcgtc acgaatgatc attctttgtc ctctgctttc attaaaacgg cgcgctcggt
     2461 tctgactaac aattttccct gcatcagaag cgcaaaagta cggatgagca gtattgcaat
     2521 aataaaattg gtaaagataa acagcggcac cgccagcgta tggaaaaatc cattatcgct
     2581 gccactcccc agatgcaaac cggtggttgc cagtgcagat acgccgaacg agaaactcca
     2641 gaatgaagca ttaaatggct gggagagata ccatggcatc agacgtagca taaacagcag
     2701 ttgcagcagt ccataaccga aaagcatttt cgccagcgtg tcaccctcgc cgccgttgac
     2761 gctcagccag gcactacagg ccaccagcgc aggagcgagc tgaatgccga gtgatgtccg
     2821 cagtgccgtg ggtaattctc ccgaactacg cagacgctgc aagatcaccg gttcaaggct
     2881 tagccatgag aaaacgcctg cgcctaaaaa caccagaccg gcgtcggtgt agcccaacgc
     2941 accacaggcc attgcgctga taaagttgtt ggcaactgtc ggcagataca gtccaggcgt
     3001 ggtagcttct tcagggtgag atccgcgcca taatcccgca gtttgccagg cggcataagc
     3061 caactgaacc acgacaccaa agctgaacag gcacaccgcc agtgggcgaa accacggaac
     3121 aaaaccaatc gccaccagca tcgtcgttgc cggaaacaaa ctcacaaaac tgctcagcac
     3181 tggatggcga acttccgcca gcacgctatg cggaaagcgt atgagtcggg caataaatgc
     3241 gctagtcaat aatccccaga tgatcatcgc cagaatcacc agcccatccc ctaaccagtg
     3301 gctgacctgc caaacctggc tggcatagcg ccaggcaaat cccatcccta tcgtccccaa
     3361 cacaatacca aagtagcctg ccggcaaatt gagcactcta tcgctctgca tcttcttact
     3421 cattttgttt aatttataaa gtatatttga aatgcatttt atggaatttt ctcgagcata
     3481 gccagagccg cagaatttgc tacggttata gtaagtcacc tgtgggaaaa cgctatgcgc
     3541 aaatatcgtc taagtgaaga acagcgagcc tttagttatc aggaagatgg cactaaaaaa
     3601 aatgtgttat tacggcagat tatcgccatt agcgatttta acgatgtgat agcaggaacc
     3661 gctggcggct ggatcgatcg cgaaacggtg ttagcacagg aaggaaattg ctggatttac
     3721 gaccagaatg ctattgcgtt cggcggaacg gtgatttccg gtaatacgcg catcaccggc
     3781 accagcgtac tgtggggaga ggtttacgca acggataatg tctggatcga taatagtgaa
     3841 atcagccagg gtgcatatat aagtgacagt gtcaccattc atgactcact ggtttgcggg
     3901 caatgtagaa tatttggtca cgccttaatt aatcaacatt ctatgatcgt cgcagcacaa
     3961 ggcttaacgc ctgaccatca actcctgcta caaatttatg atcgcgcaag ggttagcgcc
     4021 tcgcgtattg tccaccaggc acaaatttat ggcgatgccg tcatccggta tgcttttatt
     4081 gaacatcgcg ccgaagtttt tgattttgcc agtattgaag gtaatgaaga gaacaacgtc
     4141 tggctatgcg attgcgctaa agtctacggt cacgcgcagg tgaaagctgg catagaagaa
     4201 gatgccattc cgacgattca ttacagttcg caggtggcgg aatatgccat tgtggaaggt
     4261 aattgtgtgt taaaacatca tgtcttgatt ggcggaaatg ccgtggtacg tggtggaccg
     4321 attctgctcg acgagcacgt tgtaatacaa ggtgagagcc gtatttcagg tgcggtgata
     4381 attgaaaacc atgtggagtt aaccgaccac gccgtcgttg aagcctttga tggcgacacg
     4441 gttcatgtcc gtggtccaaa agtcattaac ggcgaggagc gcattacgcg aacgccgctg
     4501 gcagggttat tatgaaccga taatacgcgc gtataagttc acatcatcat aggcatcatt
     4561 caggaactca gcctgtttca ggcaaccttc aaggataaaa ccattgcgca aagcgacctg
     4621 gttgctttgc ggattgtcca cccgacattt gatcacgaag cgtctaagtt caccagactg
     4681 agcgtaatga tgaatcaatg cctgcagcgc ctgagaaatg atcccctgcc cctgatgaga
     4741 ttcgtccagc cagtagccga tttcagcagt tttattcagt ggttcaatac gattaaacga
     4801 gataacgccg ataagttcat cttctttgaa aatcatgaac attttggcat agccgcgttg
     4861 atgcaacatc acattaccct gcaccgtttt tcgcgtgtcc tcttcactct gaacgaactg
     4921 cggccagttt agcgactgct gtaaccaggt tttattttta cagattaact gataaagagg
     4981 tgtgacgtga ctttccgcaa cagcatgtaa ttcaagtgat tcgcttactt ttatcgtttc
     5041 agtcatctta tctccagtca atccatgctg ttgttaaaaa agccgcctgg ctggcggctt
     5101 aggaaagttt acacctcttt gacgctctcc tgctgcagga ttttgagcat ctcatctttt
     5161 aactgtagct tttgtttttt catgcggacc acttccgcat tgtaccctcg accgtcggaa
     5221 ccttcctttc tggcaatttc atgatcaagt ttattgtgtt tatcgaacaa ggacataaag
     5281 cgaggatttt cgtttttcag acgggatatt aaatctcggt attctggaaa catacgcact
     5341 ccctgttagt ggctgacaag gtgtatctga aatacacacc atcagattac tcatttaact
     5401 tagggaaaaa tgcgagcggg atcgtgttta accagacggc acctcccgaa gaaggtgccg
     5461 aagaaaaaac aattaactca tcacgcggtc gtcaacatac tgacgtttat ccggcgctgg
     5521 cgggaaatac tgatacagcc atgtttcact gatagcgtcc ccctgacaac gcagatacat
     5581 ccgcatatcg accggatcag tggagtccga cgtcggatac cagtcaaact gaatacgata
     5641 accatcaatg ggttcaatat agagaatttc gatttgcttc gcttccccac tggacagcgt
     5701 aatcaccggc tcaatgcctt ttggcgcggc agctttcaga tcaccaccaa cgaaatcgac
     5761 ggcaaaacga cgcgcccatt tttcgggata gtgttcacct ggcgcccaac cttccgggaa
     5821 accgcccatg ccggtacgcg tcgccataac gcgcgctaat gggcaatgaa caggcggttg
     5881 cgcactccag tacagacgat actggaatgc aaactcatca cctgctttta cagctttttc
     5941 tggctgccag aagcagacaa tgttatccag cgtttcgccc gttgttggga tttccatcag
     6001 gccgatggtg cccttacccc acttgttacg cggttccacc cacagacttg ggcgtttgtt
     6061 ataccagccc ataatgtcct gataatggga gaagtcacga tccagttgca ataaaccaaa
     6121 ccctttcggg ttgttgtcgg tgtaagcatt gaactgcaat ttttgcggat tattcagcgg
     6181 acggcaaatc cactcgccgt tgccccgcca catggacaga cgatcagagt catgaatttg
     6241 cggatgaatt gtatcgcaca tccgacgttc attagtaccg cagctgaaca tactggtcat
     6301 cggcgcaatg cccagctgtt taatgtcttt gcgcgcatac aggtgatttt ccacatccat
     6361 aatcacctga cttttctcac aatggatagt gaacttatag gcaccagtaa tgctggcgct
     6421 atcgagcaac gcataaacgg taaaggtagt tgcccccggt tttaccgtat caaaccagaa
     6481 ggcggtaaag tcggggaact cttctttact gtcggtgtaa gtgtcgatcg ccaggccgcg
     6541 ggccgacaaa ccgtattgat atgtatcatc aacggcgcgg aaataactcg cgccgagaaa
     6601 tgatactaca tcacggcgcg ccagttcggg ggctttaaac acgcgaaaac cggcaaagcc
     6661 gagatcgctt tgcccttcta attgtttggt atcaacacct gcatcgttgt atttgaacaa
     6721 ctccgggcga aagtgaattt cacgcgccag atgtgttgct ggatctacag aaaacatacg
     6781 aacgcggcga cggaatccca ttcccatatg gaagaactga gcgtccagtt gacggttctc
     6841 aacgttatgc cagagcgatt tttcggcgtc gtattgaata ctgttataag cctgcggcgt
     6901 cattgtcgcc agcgtgtcag gtaacggacg aggcgcacca cgccacgctg tttgcgctaa
     6961 gtcgtgcgcc attgactgta gaatggagaa gtcaaaacgc tgggtttgcc cgtcggcaat
     7021 atcagaatct gccgcgaatg ccgcctgaga aaaaagagaa gcaatgccgc tggtaccgca
     7081 cacggcggcc atagccattg aacctttaat aaatcgtcta cgatccatac ctgaaagtga
     7141 gtccttctgg tgttgtgaat ggtcccgcgc tggtcgtttt cagcggcaag aaagtgctta
     7201 tttatgaccg ctcactctag acaatattta ttaataatcc aattgttgag ctgagagaat
     7261 atgcataaat cggcattttt ttatgtcgat gggatgagcc tgattcgttt gtaaaatcag
     7321 gcaaattttt gcaaaggcgg atggtcagaa tttaaagcaa accacagcga cgtttactgc
     7381 gttcttccgc ttcctgataa agctggaact catccaaaac cggacaaccc aacgcctgct
     7441 caaatgcttc gcgactggca ttaccgtgat tgcgtgcctg cgtttcatta cccagatttg
     7501 actggaagat ccccgccgcg ctaacgggca agaaatcttc ataggtaatg ggttgcgccg
     7561 cgacccaacc acgttcaatt aagggctgtg gatcgtctcc gggatgaatc gcctgacgat
     7621 gcgcctcacc cgaaggcgtc agacggtacc ggaaccatgc cagcccttgc tgacgcatta
     7681 aaaactcact gtcaggaaaa gtgcggaagg tttcctgtaa atgcatttgg tgagtgagat
     7741 tatcctgccc ggttccagcg ttacgcagaa gatcatcata cagttgtcgc cctttcggcg
     7801 ttaatgccac gccacgctgc tcaatttcac caaagcgcgc agtatgcgtg ccctgtttct
     7861 gccccgcaaa caacaccgtc tcttccagtg ctttaaagct ggtctggcgt agtaaaatcg
     7921 gtacctcgcg gcgcggcggc ccttcgatca gaatttttgg ttcaattccg cattcaggca
     7981 tcatcgactg cacccggtca atatccagcg tacgtggcgt cagatggttg atatggcatc
     8041 caggaaaaca gaccacatca gcaatcaacc gatgttcgtt gtgcaaggcg cgataggttt
     8101 cttcatctac cgttgctgac tggtgccagc gaaacgtttc cagggcttcc tgcacaaact
     8161 cctgtgcctg tgtttcgtta aaaccgcccc gctgctcata ttcctctaac agtagtcgac
     8221 aacgtggggt gaagatatcg cgctgacgta gaatctccgc cgctttctgg cgcaaaattt
     8281 cgttctcgat aagctcaagg cggagtaacg aggtaaaaac gcggaaggga ttacgcgcca
     8341 gagaagcatc atcaatgggc cgaaatgctg tcgaatgtac cggcaccccc gcctgcgaga
     8401 gatcgtaata gctcaccggg tacatcccca taatggcaaa catccgccga agagtagcaa
     8461 gctcttgtgc agtcccaacg cgaatcgccc catgacgttc aacattcagt cgcgccagct
     8521 cgtctgcatt taccattttt tcgtgcagtt gaggattgtt ttccagcaca gccagattca
     8581 catcagctac cagttccagc agcgtgccgt actgcggaac ttcttgctgg tacatggctg
     8641 acattgcctg cgaaaactgt tcccgaatct catccgccgt gatgctgttc gccatattgt
     8701 ctgcctccag tggtcagtaa tctggagtgt aggtaaccgc attcactctg gcgggaagaa
     8761 tttacaaact gtgatctcgc cgcgaaaaca tcaatattat ccattttgct gtaacatgat
     8821 tgctttaatt gttaataata ttttgcaatc aagttatcat aatcaaacaa cttcacttgt
     8881 cagcgacacc gcttcgtttt taacatcgct tatggaaaaa aatagtctgt ttagtcagcg
     8941 catccgtttg cgccaccttc atacattcgt agctgtcgca caacaaggaa ctttggggcg
     9001 cgcggctgaa acccttaatt tgagtcaacc tgcgctctct aagacattga atgaactgga
     9061 gcagctgacg ggcgctcgct tgtttgagcg tggtcgtcag ggggcgcaac ttaccttacc
     9121 cggcgaacaa tttttaacgc atgcagtcag agttcttgac gccatcaaca ctgccggaca
     9181 gtcgcttcat cgtaaagaag gtcttaataa tgatgtcgtc agggttggtg cactacctac
     9241 tgcggcactg gggatattac cttcggttat aggtcagttt catcagcaac aaaaagaaac
     9301 gaccttgcaa gttgcgacaa tgagtaaccc tatgattctg gcgggtttga aaaccgggga
     9361 aatcgatatc ggcattggtc ggatgtcaga tcctgaactg atgaccgggc ttaattacga
     9421 actgctgttt cttgaatcgc tgaagctggt tgtccgccct aatcacccgc tacttcagga
     9481 gaacgtaacg ctaagccggg tgctggaatg gccggtcgtt gtatcaccag aaggcactgc
     9541 gccacgccag cattcagatg cattagtaca gagccaggga tgtaaaattc cttcgggttg
     9601 tatcgaaacg ctgtctgctt cgctatctcg tcaacttacg gttgaatacg actacgtgtg
     9661 gtttgtccct tctggcgcgg taaaagacga cctgcgtcat gccacgctgg tggccctgcc
     9721 tgttccggga catggtgcag gcgaaccgat tggaatactg acccgcgtag atgcgacgtt
     9781 ctcttctggt tgccagttga tgattaacgc tattcgaaaa tcaatgccgt tctgaaaggt
     9841 gaagggatct gtcgatccct ccttgaacat tttcacaccg tagcgaaact aactggttca
     9901 cccgctccgc gaggttttgc cgacacagaa tgtttgtgca gacggaatac atccaccgcc
     9961 tcagttaatc gtgccgcctg ttcttcaagg gacaacgccg ccgctgaggc ctcttctacc
    10021 agagaagcat tctgttgcgt caccttatct atttcagaaa tcgcctggct aacctgcgtt
    10081 atgcctctac tttgttcatc cgaggcggcg gcgatttcct gcatgatatg tgtgacactc
    10141 gcgacggcat caacaatagt gctcatggtt ttccctgccg ttgccacctc atccgacccc
    10201 aggtcaatta acctgactga ttcactgatc aagccttcaa tctctttcgc cgcttgggcg
    10261 ctgcggcttg cgagtgtccg tacttcgctg gcgacaacgg caaatccacg gccttgctca
    10321 cccgctcgcg cggcttcaac ggcagcattc agtgccagaa tattcgtctg gaaagcaata
    10381 ctgttgatga cggcggtgat ctcggaaatt ttcttcgaac ttgtagagat agcgcccatc
    10441 gtttttacta caccggaaac cgtctgcccg ccatcgctgg ctttaataga agcctcttgc
    10501 gccagtttgc tggcatgatg cgcgttatcc gcattctgtt tcaccgtcgc agtgagttgc
    10561 tccatgctag cggcagtttg ttcgatagcc gccgcttgtt cttcggtgcg agatgacagg
    10621 tccgcattgc cagctgaaat ttcgctggtg ccacgataaa tctcttccgc accctgtcga
    10681 acagtcccta ctgtcatccc cagtgaatgc tgcatttgct gtaaatgacg acttaagcga
    10741 ccgatttcat tacgacccgc cggttcatcc ttcatcgtca gatcgccact agcgattttt
    10801 tcaatccgtt gtgcggcatg ttgcagtgga cgaatgacga tccgacgtag caccataaat
    10861 gttatcagcg tcatgaccag ggcaagcaca aacgcgccaa tcatgaacat cccacccagg
    10921 cgggtgcgct gatgggccag ttccgccagt tggttggctc tggtgctacg tatcttaacg
    10981 gctttgttca aaatatcggt ataagcatta tccagcggtc ggatctgctc actttcatga
    11041 ttgataatcg cttcaaacat gccatttttg gcatatttca acataggttg catacccgtg
    11101 atataagcct gaaagcgttg atttaattca gtgtcgaggg cttcatcagc aggtgttttc
    11161 accggtcgat tctgataagc acgataacct tgctgcgact gtttaatctc cgattcggct
    11221 tgcgcaatat ttcgcttcat tgcttccatt tccgcaatac gactcgcagc cccggcctga
    11281 atcatattga tccgggctga acgcaaaaaa tctgaactgt tcgctaaccc ggttcgaatc
    11341 tcaatctccg cagtaacatc acgctgatcg cgatcggcct gccagaggaa atagccagcc
    11401 agagcagaac ttaatgcgaa tagaactaag ataccgccaa gaatgcaggc aaataacgga
    11461 accagcctga tgtgatgcaa aaaacctaat cgctgtgagg gagttgtatt cattaccgtt
    11521 gtctctcgtc caggtttact cctgatagaa agtcatcggc acttgacggt aattacttac
    11581 gactaaaagc ctctttgcgt cacattttta caacaattat tgcagagaag gttaatgcca
    11641 ttggaatgaa gagagtaacg aatgattaaa cgggctaacc cgaatgacga aaaattctgg
    11701 aaaacagcac gcaatttcgt gaagtgatta ttttcgtccc taaggaaacc tcataggatg
    11761 cctgcgggtg cctgagactt tctgttttca ggctaaccgg gaatcagaaa gacgaaggcc
    11821 ccgaccgata tttctatcaa ccgaggccaa cgaatgaaca tagcaattca gatcttagcc
    11881 tcttacccgc catccggcaa ggagaaaggc tatgaaacgc aaccctctgg tggtgtgtct
    11941 gctcattatc tgcattacga ttctgacatt cacactcctg acccgacaaa cgctctacga
    12001 actgcggttc cgggacggtg ataaggaggt tgctgcgctc atggcctgca cgtccaggta
    12061 agggcaagcg cggggatttt ccccgcgcat ctgactagca gattcattat ctcatggcac
    12121 cccttttaca cttcagttag tgcatgtttt tttcgatgca ctaatagaac catcattaca
    12181 gacaaatttg ccgcctgccg tacagtgtga gatcccccct ttcgaaccag aacagggttt
    12241 gcgcccggca tcggcataaa aggaaacaag cgttcccata aacaaaataa gtgccagttt
    12301 tttcatataa cctccatggt ttatcgaatc ttatgttcga tttgaacgtt atacgctggt
    12361 ttattttgat accgttgatg agatcaaaga tcgggtgaat tgattaaaaa taagccagat
    12421 ggacgtatgg gcaaggatta tggttttatt tgtcatacaa ataaatataa taggcgcttc
    12481 cgatgtagac ccgtattctt cgcctgtacc acgggtcggt tttagtacag gcgttttctt
    12541 taaatatcct tcaggaacgt ttacgcggca tcatgcgtag aagtgtgttg tccttccaga
    12601 aatagtggtg tgccagtgca gccgcagcgt gcaacccgat gacaaaatat cccagattcg
    12661 ccagcgtttc atgccacgac tttaagctat ctacccgttc gaaattggcc tctgaagcgt
    12721 aaggcatcgt caaaccaaac gcaaaccacg ggttgccccg gttatacatc atcaccaaac
    12781 caatcactgg cagcgcaata aacaggagat aaatcaccaa atgtcccaaa tgcgccagtc
    12841 ccgtcatcat cggttttggc ttaggtataa tcggcggggt tgggtatttc agccttaaca
    12901 aaagacgaac gaccatcagc acgagaattg agatgccaca ggaaacatga atcatgttga
    12961 tgagtggccg atcgctacgt gggaagaaac cacgaaactc cattgcgcaa tacgctgcga
    13021 taaccagtaa aaagaccagc cagtgaatgc tgatttgtaa ccttgaatat ttattttcca
    13081 taacatttcc tgctttaaca taattttccg ttaacataac gggcttttct caaaatttca
    13141 ttaaatattg ttcacccgtt ttcagtacgt tacgtttcac tgtgagaatg gagattgccc
    13201 atcccgccat cctggtctaa gcctggaaag gatcaatttt catccgaacg ttcctgacag
    13261 gagaaaacca tgagtaaagt tggtattaac ggttttggtc gtatcggtcg actggtgttg
    13321 cgtcgattac ttgaagtcaa aagcaacata gacgttgtcg ctattaatga tctcacttcc
    13381 ccaaaaattc tcgcctacct gctgaaacat gattcaaact acggaccatt cccctggagc
    13441 gttgatttta cggaagattc acttatcgtt gatgggaaaa gtatcgcggt ttacgccgaa
    13501 aaagaggcta aaaatattcc gtggaaagcg aaaggtgcag aaatcattgt cgaatgtact
    13561 ggcttttata cctccgccga gaaatcgcag gcgcatcttg atgctggtgc gaagaaggtg
    13621 ttgatttccg cccctgccgg tgaaatgaaa actatcgttt ataacgtcaa tgacgacact
    13681 ctggatggca acgacaccat tgtttccgtg gcgtcatgca ccactaactg tcttgcgccg
    13741 atggccaaag ccttgcatga cagtttcggg atagaagtcg gcacgatgac gaccattcat
    13801 gcctatactg gcacccagtc actggtggat ggcccgcgtg gtaaagattt acgtgcttca
    13861 cgcgcagcgg cagaaaatat cattccccac actacggggg cggcaaaagc cattggtctg
    13921 gtgatcccgg aactgagcgg caaactgaaa ggtcatgcgc aacgcgtgcc ggtgaaaaca
    13981 ggttcggtca ctgaactggt atcgattctc ggaaaaaaag tgactgccga agaggtgaat
    14041 aacgcactta aacaagcaac caccaataac gagtcatttg gttataccga tgaagaaata
    14101 gtctcttccg atatcattgg cagccatttc ggttcggtgt ttgatgccac gcaaacggaa
    14161 attaccgccg tgggcgattt acaactggtg aaaacggtcg cctggtacga taacgaatat
    14221 ggcttcgtca cacagcttat tcgcaccctc gaaaaattcg ctaaactctg acgcgcacag
    14281 gcggaggaaa aaacctccgc ctctttcact cattaagact gtaaataaac cacctgggtc
    14341 tgcagatatt catgcaagcc atgtttacca tctgcgccgc caataccgga tttacgccat
    14401 ccggcgtgga agccttgcat agcttcgaag ttttcacggt tgatgtaagt ttcaccaaac
    14461 ttcagccctt taatggcttt catcgcgacg ttcagatttt gggtatagat tgatgaggtc
    14521 aggccgtaat cactgtcatt agccattgag atagcatctt ccagcgtgtc aaatgcgacc
    14581 accggcagaa ccgggccaaa ggtttcctca tgcataatcg acatttcctg gcgaacatcc
    14641 agcagcaatg tcggcggata ataatatcct ttcccgtcta ctgctttgcc acccaacgcc
    14701 actctcgccc cttcttctac tgcgcgcgcc actttttgct cgaccctttc cagcgccgcg
    14761 gcgttaatca acggccccat cgcaatgtcg ttgcgttcag cggggttacc aaattgaacc
    14821 gcctgcatcg cttcacccag ccgattgacg aactgatcat aaatgccttt ctgtacataa
    14881 acacgttctg cacagttaca cacttgccca ctattaatga cgcgtgaatc aacgatggct
    14941 ttgactgcca gttcaagatc ggcatcgtcc attacgatag ctggtgcttt accacccaat
    15001 tccaggcaca ctttggtgat gtttttcgcc gcagtcgcca taatcttctc acctgcagag
    15061 acgctgcctg tcatactgac cattgcgacc tttgggttac ccgccagttc ttgcccaacg
    15121 gtttcaccac gccccagtac aaggttaaac acgccgcgcg gaaggcctat ttcatcgacg
    15181 attttggcga atgcaatcgc attgtttggc gtaaattcac tgggtttaat gacgatggta
    15241 ttaccagtca aaagagcggg agccattttg cgggcaatga ggaagaacgg gaagttccac
    15301 ggcagaatgc cggtagtcac accaagcgca cgtttaaaca gaagaatatt ttctcctgga
    15361 cgatcgcttt gaataatctc gccctcgtaa cgccgtgccc actccgccat gtaatcgata
    15421 tagtcggcag taaaagccac ttcgacttca gccagctgct ggatcttgcc cccttcttca
    15481 acaatcagcg cactgatttc actggcgcgt tcgcggatcc cggcggagat tttgcgcaac
    15541 caactggcgc gttcaatagc aggcaacgct tcccattctg gttgtgcatg ttctgctgca
    15601 tcgattgcct tacgggcatc ctcggcctga ccatcgggta tgcgggaaat gacagcctct
    15661 gtagcagggt ttaccacatc aatccatgcg tctccacgcc aggtaacaaa ctgtccatcg
    15721 atatacatag gatgttgaac gggtactgac atgggcgact cctgtgattt atatgtttgt
    15781 tttcggtaaa tacattgtta attaacatca atcatcagag agacggcatc gtgaaaggac
    15841 aattctgtga agggcttcat aaaaacaagt gtaaaaggaa taaaaggcag aaataaaagt
    15901 caggagtcat gcccgtttgc aaacaagtgg ttcacaaacg ggcaattttt aacgtaacga
    15961 gcgactttcc atcgcctcga taagcaccgc atcgtgtttc agcgtttgcc atgcatggat
    16021 gacttctgcc ggaaaatcaa cgtgaacgat aaaatctcgc ccgcggggac cgtagccatc
    16081 gctatcatcg cgtaaacgcg gcagctcgcc agtgagtagt gatagataac gctcaaccgg
    16141 ccataatcct tgcaacgggt taacaaagat tacactgtct gcgttatttc ctaactgagg
    16201 aacgaatccg ggataactta accagcgtgg tgcatcggga ttgtccccag tcatacggcg
    16261 gaacgtcgcc atcgtgcgcc gctgcatggt ggggtcctga acaacgatag ccgtatgaac
    16321 tcgttctacg gcctgattca atagcgcgat gctaaagcgt gcgttttcac cgcagtttgt
    16381 tgactggtct tcaatccaga ttttttcatg cggaatgtgc cagaactgat gagcgatatc
    16441 cgccaggatg gtcgcttctg ctctgccagt ggtgcggata gtgttgtagt gcggatgctg
    16501 tgcgatggcg ctatacaaaa aagttgtcga gtgaccgata ccaccactaa tcagtaaagg
    16561 aatttgctga tcgcgagcaa tcttacatgc cgcatcgata gtcggcataa ccgcatttcc
    16621 tgcaagtatg acgcaatcag cctgatacgg cacctcaccg gagaaatcat cctgcgccag
    16681 ccactgtccg ataacattta tggcatctat agttgccgtc gaaagcgtcg gaaacggggt
    16741 aatgttcatg gttcctcctt atcgttccct taagaatact gcgtgagata atagtgagaa
    16801 gcggagcact ctgaaaggag gaagtcgtat ttccttaacc gccactccgc aagcctgaca
    16861 tatcttttaa ggaaatgctg tagataaaca ttattcatct tgcagctgag aattgtattt
    16921 ttctgaattc ctgaattttt gtccttaccg ttttagaatt aataaaaaac ccggcgctaa
    16981 atgcttaccg ggttttagat ttatcaggca aatagcaggg ttaaccgcta atctgctcca
    17041 tcgcctgcaa aatacgctta tctgaaatcg gataaggcgt accaagttgt tgagcgaagt
    17101 aactaacgcg cagctcttct atcatccaac ggatctcttt cacgtcttca tcctcacgac
    17161 gtgcaggcgg cagtttgttg atccattgct gccacgcctg ctggacgttt tcgactttca
    17221 gcatctgagc acggtcgcga tgtggatcaa ccgccagttt ttccagtcgt ttttcaatcg
    17281 cctgcaaata tcgcagcgtg tcgcccagcc gtttgaagcc gttaccagtg acaaaaccgc
    17341 gatataccaa cccgcccatc tgcgctttaa tgtcagaaag ccccagcgcc atggtcatat
    17401 ccacccgccc tttcagacgt ttgttgatat tgaacactgc cgtaaggatt tgctcgacct
    17461 gcttcgcaat atccaccacc gtgtcgttca gttcggcacg cactttttca tgcagcgcag
    17521 caaagccttc ttccgtccag accgggccac cattggcgtc gatcaattta tccacaccgc
    17581 aggagataca gtcgtcgatc agctccagca ctttgccata cgggttaaag tacagcccca
    17641 gcttggcttt gtttggtaac ttctcatgta aatacttgat cggcgaggga atattcagca
    17701 gcagtagacg gcgaagaccg ttccacattg cctgtttttg ctctagcgga ttatcaaaca
    17761 gtttgatcgc cacactgtcg cgttcatcca ctagcgctgg ccacgccttc actttgtagt
    17821 tgccacgctt ctgttcgtag ctttccggca gctgaccaaa actccagata tgcagcccgc
    17881 tctgctcgat accgtcatcc gccaccgcag acagcgtttc ctgcactttg cctttcagcg
    17941 catctttcag atcttgtagc gagcgccctt cttttagctt cttgtttttg tcatccacca
    18001 cgcggaaggt gattttcagg tgatcgggca cctgatccca gtgccagtct tcgcggtcaa
    18061 cggtaacgcc ggtcatccgc cgtaactcgc gctcaaggct gtcgagcaac ggtaactcca
    18121 gcggtgtgac gcggcctaaa aacgcttcgg cgtagtttgg tgcaggcaca aaattacggc
    18181 gtaccggttt cggcaacgat ttaatcagag caatcaccaa ttcgcggcgc agaccgggga
    18241 tctgccattc aaacccgctt tcctcaacct ggttaagtaa cggcagcgga atatgtacgg
    18301 tcacaccgtc cgcgtctgcg cctggctcaa actgatagct caaacgcagc ttgagattgc
    18361 cctgatgcca gaagttcggg taatccagct tgctgatttt ttccgcccct tctttgatca
    18421 acatgctctt ttcaaagttg agcaaatcag gcgtttcgcg gctgactttt ttccaccagc
    18481 tatcgaagtg acgagcggag attacatcgt ggctgatgcg ctggtcgtag aactcaaaca
    18541 gcgtttcgtc atcaaccaga atatcgcggc gacgtgattt gtgttccagc tcttccactt
    18601 cggcccgcag tttcaggttt tcacggaaga atgcgtgacg cgtctgccag tcaccttcca
    18661 ccagagcgtg gcgaataaag agttcacgac ataacgccgg atcgatctgg ctgtagttga
    18721 ccttacgcgc agcaacaatc ggcaaaccat aaacagtgac tttttccgtt gccatcaccg
    18781 cgccctgcgc ccgttcccag tgcggttcgc tgtaggtgcg tttaatcaaa tgctgagcaa
    18841 ctggctccac ccattccggg tcgatacgcg cagcaatgcg cccccacagg cggctggttt
    18901 ctaccagttc cgccaccatt acccatttag gcggtttttt gaataaacca gaaccgggga
    18961 agatggagaa acgcgcgtta cgtgcgccgg tatattcttg tttatcggca tctttcatgc
    19021 cgatatggga aagcaaaccg gtcagcaacg caatgtgaat ttcgcgatac tccgccggtt
    19081 cgctgttaac cggaatgcca agttctttca ccacctgacg caactgggtg tagatatcct
    19141 gccattcgcg cacgcgcaga tagttgagat aatcggtacg acacaggcga cggaaggcgt
    19201 tggaagaaag cgccttttgc tgctcgccaa gataattcca cagattcaca aacgcgagaa
    19261 agtcagactc tttgtcgtgg aagcgacgat gtttttcgtc cgatgcctgc tgtttgtcca
    19321 tcggacgttc gcgcggatcc tgaatggaga gcgcggacgt gataatcatc gcctcacgca
    19381 cgcagccatg tttttgcgct tccagcacca tacgcgccag acgtgggtcg acaggcaact
    19441 gcgagagctg gcgaccgagc ggcgtcagtt tataggcgct ggcctgttca tcagtggtga
    19501 tcgcgcccag ctcttcgagc agacgcacgc catcctggat attgcgttta tccggtgctt
    19561 cgacaaacgg gaacgcagcg atatcgccca gccccagcgc ggtcatctgc aaaataaccg
    19621 aggccaggtt ggtacgcaga atctccggat cggtaaactc cgggcgcgag aggaaatcgt
    19681 cttcggaata gagacgaata cagatccctt cggacacacg accacagcgg cctttacgct
    19741 gattggcaga cgcctgggaa atcggctcaa tcggcaaacg ctgcactttg gtgcgatagc
    19801 tgtagcggct gatacgcgct gtaccggggt cgataacgta tttaatcccc ggtacggtca
    19861 gcgacgtttc cgcgacgttg gtcgccagca caatgcgccg tccgctgtgc gactggaata
    19921 ccctattttg ttcgctgttc gaaagccgcg cataaagcgg caagatttcg gtatggcgta
    19981 agttcagctt gttcagcgca tcggcggtat cgcggatttc ccgctcgccg ctcataaaga
    20041 tcagaatgtc gccatgactt tcctgactca gttcgtctac ggcgtcaaaa atcgcctgca
    20101 actgatcgcg ctcggtgtca tcggcttctt caacaatcgg gcgatagcgc acttccaccg
    20161 gataggtccg accggagact tcaataatcg gcgcattatt aaagtggcgc gaaaagcgtt
    20221 ccgggtcgat agtcgcggaa gtgataatga tttttaggtc aggacgccgc ggcagcaact
    20281 ctttcaaata gccgagcaaa aaatcgatat tcaggctgcg ttcgtgcgct tcgtcaataa
    20341 tgatagtgtc gtactgcatc agcaggcggt cttgctggat ctccgccagc aggataccgt
    20401 cggtcatcag cttgaccatc gtgttatcac ttacgtgatc gctgaaacgc actttgtaac
    20461 cgatgcaacc gcccggctcc gttttcaact cttccgcaat acggttcgcc actgttcttg
    20521 ccgccagacg acgcggctgg gtatggccga tcagtccttt aatcccgcgc cccagctcca
    20581 tacagatttt cggtaactga gtcgttttac cagaacccgt ttccccggcg acgatcacca
    20641 cctggtgatc acgaatcgct tcgagaatgt cctgtttttt ctgactaacc ggtaaattgt
    20701 caggataagt aatttccggt cgtgccgctt cacgcagcag gactttccct gccgcctggt
    20761 caatctcttt cgccatctcc tggaaaatgg cctgttgtgc atcaggattt ttaaccttct
    20821 tcacgccgtg cagacggcga gaaaaacgca gtctgtcacg cagcatcagc gaatccagcc
    20881 gctgctgcaa ggccgtaaag gtcaattttt gttgttctgt cataacgtta gtgggcagta
    20941 gtctgcctga ttaattttct tttaaatgat agatatagag taccacatcg cggctttcta
    21001 tgcgttgttc aataaattcg aacataggct tcgatttatt gcgctatctc tgtggcagga
    21061 ttgtgaataa agtgtcaaca agcaacgggg catcgcccat tcaaacatct ataaggaaac
    21121 accatgagca aggtattagt tcttaaatcc agcatcctgg cagggtactc tcagtctaat
    21181 cagttgtccg attattttgt tgaacaatgg cgcgaaaagc actccgctga tgaaatcacc
    21241 gttcgcgacc tggctgcaaa tccgattccg gtactggatg gcgaactggt tggcgctctg
    21301 cgtccgagcg atgcgccgct gactccgcgt cagcaggaag ctctggcact ttccgatgag
    21361 ttgattgccg agctgaaagc ccacgacgtt atcgttattg cggcaccgat gtataacttc
    21421 aacatctcga ctcagttgaa aaactatttt gacctggttg cccgcgcagg cgttactttc
    21481 cgctataccg agaacggtcc ggaaggtctg gtaacgggta aaaaagccat cgttattacc
    21541 agccgcggcg ggatccacaa agatggacca acggacctgg tgacgccgta tctgtccacg
    21601 ttcctcggct ttatcggcat taccgatgtg aaatttgtct tcgccgaagg gatcgcatac
    21661 ggtccggaaa tggcagcgaa agcacagtct gacgcaaaag cagccatcga cagcattatt
    21721 tctgcataac cggacacggt cttgatgtgg actgtagata ttcagtccac atctcaatcc
    21781 acttacctgt tttcccataa tctcagcatc gctttgtgct cgtctgtcag cacaatctgc
    21841 gggcgtctgg ctcgaatata gctaatcgct tcattaacgg ttttacagtg tccgtaacat
    21901 aacaaccatg ccgccaccac cagcgcactg cgcgataatc ccaatgcaca atggaccaga
    21961 acgctgcctt gctcttcgcg taatgtttcc agcatcgcca cggcctgtcg gagctccccc
    22021 tcttccggaa ccaccagatc cagcatcggt acacaaaaat agagtcgatc ttttgtggct
    22081 cgtccgcgag ggaattcaaa ggtgacgtcc agaaccgcat tctgtgccgg aatatgtcgt
    22141 ggaaacgccc ctaaataaac accagcagta attttgctca ccggctccag gcgaagacaa
    22201 gaccaacgca tagacagcca catcccgata cgccagggca atgtcagcca gtaaacggcg
    22261 ggcgggagtt tcccctgact atctttccct gttgttatcg cgccaagccc accgtaccca
    22321 cggccaatga tgaatagcga taatactggc caacataacc agactgacca ccataactga
    22381 agcattatca ttagctccat caacacaatg cacgagcccg cgcctacgac atatggcagt
    22441 gctattttga ttcgacgttg atcaggtttc tgataattcc aacgacggtc gacgggcacc
    22501 atccagtcaa ttaacatacc taccgccagc cccgtgatga catcaataaa atgatgctgc
    22561 caggtcgtca gcgtcgaaat ggcgatgagt aaaaaccatc cgccgcagac tttacgccac
    22621 ctcacagcca gatgctgacg aaagtgacgc caaagtagcc agcagagaat aatatgcagc
    22681 gaaggagact ggttataagg gttcaagttg cgaaaatagc catcctgtca ccccactcac
    22741 ttcaggacgg ataaaactaa acttcagcgg gtagagaaaa aaaccgcagc aggccattac
    22801 cgttgccaga ataagccggt ggacaagtcg gcgctgttcg aatgtcgtgc tacaaacgaa
    22861 cagcgaaaat ccatataaaa gatccagact ccagtaagga acaatagtcc agggaagaaa
    22921 agggatcgcc gtttcccaac cgaatacctg actgggaata tcatggctgt taaggtcctg
    22981 aaccgcggtg aactgattaa gagatccata ggtgaagaag aaaaacgggg ccagcaacaa
    23041 taaccagcca gcgccttgta gcaatacgtt ccgacgttca gttatcacga cgcaccgcca
    23101 tcgaaaccgt aaaaataccc cactcatcaa tccgttgtgt gcatttatca aatccggcat
    23161 cacgcacgag tgaatccatc tccccttgcg aacgcacgcg cattacccac ggtttaccat
    23221 ctttatgact ggttaacacc ccggcaatca tctccagttg agggtgccac ggctgcccgg
    23281 tgtagatgag aatgccgccc ggttcgatgg cattggcaag acctgcgagt gagtttttta
    23341 cctgctcatt ttcgggaaac agctcataca gtccagagac aatcgccagc gtaggccgcg
    23401 gagttaacgc gctgagttcc tccgggttaa acgcatcgcc ctgttcaaaa cgcacccgcc
    23461 cagacattcc ccgttgggca atcatctctt gcccctgtgc aacatttaac tcactgtaat
    23521 cacgtaataa aatatcgctt acggcaggct cgtttgccag tgcatccagt acatagcgcc
    23581 catgccctgc ggcaatgtca accacgcgga cggctaaacc tttggcgtgg agatcggcaa
    23641 cggcttgttt aatcagtatt tgtaaatggg ttttgcgctg gcgaataccg cgccagccaa
    23701 cactgttcag gtagattttg tcgattaagc gcccgaatgc gttactacct tgcggttgat
    23761 tttgatagac ataatccagc gaactgcctg aatcaaagcc ggtgctcatt ccgagatgca
    23821 ggcccgaaga gtgcgtcccg aacaatttca tcgcctttcg cataaagcga tacgctaaat
    23881 caaccggcga taatggcacg ggtccaccag aaagtagccg ccatcgatcc gctgatggtc
    23941 ctgtgcagtc ttcatgctga taatcaaatt tttgcgattt gttagcatat aaacgactaa
    24001 taaagctttg cattttttca aatgccagcg ccctgttctc ttcacccaac gtgtcgtgat
    24061 aaaagcctgg cagcagatgc agctctttca gagggctacg taatctctga taaaaatcaa
    24121 tctgcggttg gcgatgcacc acatagtcat cgcctgatat cagatgttgc gtggggagcg
    24181 taatcgccgc cgcatctcta ataatacgtt cagacgtttt gtagagatcg agcaagatat
    24241 taacggcaat cgcccgtgtg atcagcggat cattattgaa actcgccccc cgttgccgat
    24301 cgtgggtcaa atagcgtcct ttcacatagg aattaataaa aaacagacca cgcagacgat
    24361 gccataacgc cagcgcagga cgtgccagcg gcacatacaa tttaacctta aaggccggag
    24421 aagccagcac cagcccgcga attgcaggtg cataatcatg aatccatgtg gcaaccagca
    24481 ctgcgccgac gctttgcgcg atcactacca cctcttccag tccaacctgg ctgtcgctgg
    24541 cagcaaaacg gacaaattca tcgacatccc gcactgaacg cgcaagagat gggctataac
    24601 cacgcggccc cgaacttttt ccatgccctc gggcatccca ggcataaaaa gcagtatctg
    24661 gcatcgccag ttcatcaacg agatgttgta gacgaccaga atgttcatgc ccgcgatgaa
    24721 ataagacgat gacctttttc gccccgggct gtaaagcggg ccagtgccga taaaacaacg
    24781 ctgtattatc actggtggta aaaaaatgtt ccccagggat gcgtgaattt tccattttct
    24841 ctattttcct tcagtaacag cagtagcgta taaaataaaa aaataccgga gcggtgaaaa
    24901 ttaatgagtc aatcctgtca aggagtccgc catgtcctgg caatagtttt ccgctatctt
    24961 taacaccaat atctcgtttg atggctgaca tcacgacatc gccgcaaaaa ccactaatac
    25021 caattaacaa acccgctaat aatgcctgta atgtatttag cggtgtcagt aacggtccga
    25081 taatcagtga cgcaatcatg atggtgatga cgccgcccat cagaccttcc aatgtttttc
    25141 ccgggctgac tttagggacc acttttcttc tgccgcagga ttttccccat aaatactgtg
    25201 caatatcgtt tgactcggtt aatgccaaaa gaaataacac cagtaacgcc ccgccctgga
    25261 tatttgtggt tggcaacatt aataaccagg cggcatgact caaggcgaaa acggtcatta
    25321 tccagcccca aaaaatggcg ctcacggtat gcagaaaacc agaggggtca cccactaaaa
    25381 cttgcccggt ggctaatatc agaaacccag ccaaaggtat aaataacaga aacagctcaa
    25441 agcaattaaa accgataagc aaatagttaa gaggaatacc ccaatataac caacgcggaa
    25501 aatgtacaga tataagcgta caatattctt tcaatgccag aaaacttatc agcgcaaaaa
    25561 acgtcaacgt catccagcgt ggacctgaaa tcaccattga aaaacaaatg acgatgcccc
    25621 accaggtacg aatacgtaat gtaacctcac ccccttttct ctccggtaac cgccatagca
    25681 gaaaacggtt tatcagcgtt gctaaaatta acaacgcgaa aagtgttgcc agagattttt
    25741 ccagcattat atttcagcgc tcataagaag cacactccga caacgattaa tcgcagtcca
    25801 gagaagcaga attgaagcaa tgctccataa aagattattc cactgcatcc actgtggata
    25861 aatggcaacg gccagacccc acagaccaaa tattaacgcg cgatcgcttt tgccaaatgg
    25921 accggcataa ctgcgaacac cgttaatcgt ctgcgcgagt aaaccgcaaa actcggtcaa
    25981 tatggtgcaa aataacatga gtatcacgag tgatgcgtta ctttccggta aaaataaaaa
    26041 tgttaaatag agcgcaatat cggaaataac atcaccagtc tcattcaaaa ttgccccaag
    26101 ccgtgtttgc tgattgcact cacgcgccaa catgccatcc agcgcattga gcgccatacg
    26161 gataaaaagc acgatgggca atagcataaa gaggatgggt tgtgccacca acatcaacag
    26221 taatccggta agaagagaaa gcgccagtgc agcaagagtg atgtgattcg ctgtaacgtg
    26281 gtgcttgtaa agccaaaaca tcgtcggcct taacagcgac tgaaagagcg gttttatctg
    26341 gtagagcgtc attatcaatc cttgataatt cggtttgcgc tatggtgcat caattcccct
    26401 gacggagata tcgcaacacg ccgcttcatt aaaaattcag aatatatcaa ggtattgggg
    26461 actcactgtc aggaataatt atccccaatt aatcaaaggg ttaagaaata ttacgttgtg
    26521 gatcaataca actcatcaag ttttctcatc gcttgttccc gtattgcgcg cgtcttaccg
    26581 ttaagcgcca gctggcggac taaatccagt tcagggttaa caagctctaa cgccagcaaa
    26641 ggatcacgtt tcagcctcgc ctttaataca tcaactggaa aacggggatt attcatcgcc
    26701 acaatccacc actcgggatc aatttctgcc gccagtactg caggcggtgt gtggcggttg
    26761 ttaagtgcgc cgaacttgaa aaatgtgaga ttgcttttcg ccagtgtact ccaccatata
    26821 cgatccggtt gttttgccag tacatccagt tgtgctaacg catcggggtc gagtgcataa
    26881 tagacgcgta atgcttcatc aacctgcgca cgggagctgt tttcctgcct cagaaaaaac
    26941 ttcgcccaaa tatcattgcg tggcgtattt tgctgtaatt ccttgaggac aacagcgggt
    27001 aaattatccc aactaactaa tgcactctct aaaaagtccc agtcttccaa acgtatcagg
    27061 gctgtggcat ttaatagcat ctgatcatca acttcaccgt ggctgataag ctcagacaaa
    27121 atcatatcag cgctggtttc atacgcagct gaatctgctt tcggattatt tttccgatgc
    27181 gataaagcac gttgccaaat tttttgtttc atcgacgtgc tcagatactg atttttcgcc
    27241 agattactcc acccctcagg atcgttgaaa cgtgtcaata accgttgtag agcagcgtca
    27301 gagtgaatta cctgcatatc ccgacagcaa aagtaggcag gggatttttt ctccatcctt
    27361 ttaacaaaat taaactgtaa gttatctggc aatgctaaaa ataacacacg tattagatca
    27421 tcgtcattct cgaatttttg gtataaatag gctgcaactt tgtcactctc ttgtggcgtc
    27481 atccgcaata attgaccatg gtgtttcatc tctgccagct tgaacgctaa gcttgtgaca
    27541 acattgtttg atgaatcgtt cgccaactgc attgcctgat tgtaggtgag ttgtcctcca
    27601 gcaccgacct gacgaataga ggaatcatca ctgcggatat agccatcaat aatggattca
    27661 ggcaataacc acgcatcttt ataaagctcc tgaatgatca tatcccaatc tcttttcgcc
    27721 agagtcatta cgatatccag agggagattt tcacgtagcg caagattcaa cggaccgaca
    27781 ccatcaggta gacgactcat tcgcgtcatt atttcgacag agagcggttc acggtcgagc
    27841 aaccccagaa caacaggctg gtaacccaat tgctgatcgg tcaactccag aatctcattg
    27901 tcaggcatat atctcgccat actttcaatc gtttgttcat cattgctatt aataagttgt
    27961 ttgcgtaggt tagctgaaat tcctttaagc cttgggagag ttcggattat ggcaagatca
    28021 aatgtcgcaa tatctcgtcc ttcaaatgca gcaacgttgc cttcatccag catttgcaat
    28081 tcctgca
//
LOCUS       DAPHBJ010000044        27460 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.044, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000044 DAPHBJ010000000
VERSION     DAPHBJ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.044"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            181..2604
                     /gene="pgaA"
                     /locus_tag="RHZ76_001673"
     CDS             181..2604
                     /gene="pgaA"
                     /locus_tag="RHZ76_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HDV2812326.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2613..4631
                     /gene="pgaB"
                     /locus_tag="RHZ76_001674"
     CDS             2613..4631
                     /gene="pgaB"
                     /locus_tag="RHZ76_001674"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HDV2812327.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            4624..5949
                     /gene="pgaC"
                     /locus_tag="RHZ76_001675"
     CDS             4624..5949
                     /gene="pgaC"
                     /locus_tag="RHZ76_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HDV2812328.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            5951..6364
                     /gene="pgaD"
                     /locus_tag="RHZ76_001676"
     CDS             5951..6364
                     /gene="pgaD"
                     /locus_tag="RHZ76_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HDV2812329.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAIVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6414..7202)
                     /gene="phoH"
                     /locus_tag="RHZ76_001677"
     CDS             complement(6414..7202)
                     /gene="phoH"
                     /locus_tag="RHZ76_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HDV2812330.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7821..9092)
                     /gene="efeB"
                     /locus_tag="RHZ76_001678"
     CDS             complement(7821..9092)
                     /gene="efeB"
                     /locus_tag="RHZ76_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDV2812331.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKPDLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(9098..10225)
                     /gene="efeO"
                     /locus_tag="RHZ76_001679"
     CDS             complement(9098..10225)
                     /gene="efeO"
                     /locus_tag="RHZ76_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HDV2812332.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10283..11113)
                     /locus_tag="RHZ76_001680"
     CDS             complement(10283..11113)
                     /locus_tag="RHZ76_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HDV2812333.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(11655..13163)
                     /gene="putP"
                     /locus_tag="RHZ76_001681"
     CDS             complement(11655..13163)
                     /gene="putP"
                     /locus_tag="RHZ76_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDV2812334.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFSSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(13322..13531)
                     /locus_tag="RHZ76_001682"
     CDS             complement(13322..13531)
                     /locus_tag="RHZ76_001682"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812335.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            13586..17548
                     /gene="putA"
                     /locus_tag="RHZ76_001683"
     CDS             13586..17548
                     /gene="putA"
                     /locus_tag="RHZ76_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HDV2812336.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTQAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFGNETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKVNIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGYLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(17588..18226)
                     /gene="rutR"
                     /locus_tag="RHZ76_001684"
     CDS             complement(17588..18226)
                     /gene="rutR"
                     /locus_tag="RHZ76_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HDV2812337.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            18514..19605
                     /gene="rutA"
                     /locus_tag="RHZ76_001685"
     CDS             18514..19605
                     /gene="rutA"
                     /locus_tag="RHZ76_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HDV2812338.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            19605..20297
                     /gene="rutB"
                     /locus_tag="RHZ76_001686"
     CDS             19605..20297
                     /gene="rutB"
                     /locus_tag="RHZ76_001686"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HDV2812339.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFAHIA"
     gene            20309..20695
                     /gene="rutC"
                     /locus_tag="RHZ76_001687"
     CDS             20309..20695
                     /gene="rutC"
                     /locus_tag="RHZ76_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HDV2812340.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            20703..21503
                     /gene="rutD"
                     /locus_tag="RHZ76_001688"
     CDS             20703..21503
                     /gene="rutD"
                     /locus_tag="RHZ76_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HDV2812341.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            21513..22103
                     /locus_tag="RHZ76_001689"
     CDS             21513..22103
                     /locus_tag="RHZ76_001689"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HDV2812342.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFT
                     GSTLKSNLLINIGYGDSSKLYARLPRLSFEEACGLL"
     gene            22114..22608
                     /gene="rutF"
                     /locus_tag="RHZ76_001690"
     CDS             22114..22608
                     /gene="rutF"
                     /locus_tag="RHZ76_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HDV2812343.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPAFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            22629..23957
                     /gene="rutG"
                     /locus_tag="RHZ76_001691"
     CDS             22629..23957
                     /gene="rutG"
                     /locus_tag="RHZ76_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HDV2812344.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDYTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(24215..24388)
                     /locus_tag="RHZ76_001692"
     CDS             complement(24215..24388)
                     /locus_tag="RHZ76_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDV2812345.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            24761..25357
                     /gene="wrbA"
                     /locus_tag="RHZ76_001693"
     CDS             24761..25357
                     /gene="wrbA"
                     /locus_tag="RHZ76_001693"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDV2812346.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVAIPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            25378..25605
                     /locus_tag="RHZ76_001694"
     CDS             25378..25605
                     /locus_tag="RHZ76_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HDV2812347.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(25643..26884)
                     /gene="agp"
                     /locus_tag="RHZ76_001695"
     CDS             complement(25643..26884)
                     /gene="agp"
                     /locus_tag="RHZ76_001695"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HDV2812348.1"
                     /translation="MNKTLIAAAVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPYTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(27177..>27460)
                     /locus_tag="RHZ76_001696"
     CDS             complement(27177..>27460)
                     /locus_tag="RHZ76_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812349.1"
                     /translation="LDTDPEKRINEVKNFLTALSDSICSLFIVNSNVASISLGNESFS
                     TDEDLEYGYLINTGNHYDVYLPPELFAQAYKLKNKEMNAQLDYLNRYAI"
BASE COUNT         6717 a         7100 c         6953 g         6690 t
ORIGIN      
        1 tcgggcgaga aaaggatttt atatggacac tctgctcatc atttcttctt ctcatcatca
       61 acaattcacg tctctcttcc gcgtttaata acggattatg aggtgcaaaa atatctttct
      121 tttcagttac ctgtaattag atacagagag agattttggc aatacatgga gtaatacagg
      181 atgtattcaa gtagcagaaa aaggtgcccg aaaaccaaat gggctttgaa acttcttact
      241 gccgcatttt tagcagcgag tcccgcggcg aagagtgctg ttaataacgc ctatgatgca
      301 ttgattattg aagctcgcaa gggtaatact cagccagctt tgtcatggtt tgcactaaaa
      361 tcagcactca gcaataacca aattgctgac tggttacaga ttgccttatg ggccgggcaa
      421 gataaacaag ttattaccgt ttacaaccgc taccgtcatc agcaattacc agcgcgtggt
      481 tatgcagctg tcgccgtcgc ttatcgtaac ctgcaacaat ggcaaaactc gcttacactg
      541 tggcaaaagg cgctctctct ggagccgcaa aataaggatt atcaacgggg acaaatttta
      601 accctggcag atgctggtca ctatgatact gcgctggtta aacttaagca gcttaactct
      661 ggagcaccgg acaaagccaa tttactcgca gaagcctata tctataaact ggcggggcgt
      721 catcaggatg aattacgggc gatgacagag tcattacctg aaaatgcatc tacgcaacaa
      781 tatcccacag aatacgtgca ggcattacgt aataatcaac ttgctgccgc gattgacgat
      841 gccaatttaa cgccagatat tcgcgctgat attcatgccg aactggtcag actgtcgttt
      901 atgcctacgc gcagtgaaag tgaacgttat gccattgccg atcgcgccct cgcccaatac
      961 gctgcattag aaattctgtg gcacgataac ccagaccgca ctgcccagta ccagcgtatt
     1021 caggttgatc atcttggcgc gttattaact cgcgatcgtt ataaagacgt tatttctcac
     1081 tatcagcgat taaaaaagac ggggcaaatt attccgccct gggggcaata ttgggttgca
     1141 tcggcttatc tcaaagatca tcagccgaaa aaagcacagt caataatgac cgagctcttt
     1201 tatcacaagg agaccattgc cccggattta tccgatgaag aacttgcgga tctcttttac
     1261 agccacctgg agagtgaaaa ttatccgggc gcgctaactg tcacccaaca taccattaat
     1321 acttcgccgc ctttccttcg gttaatgggc acgcctacga gcatcccgaa tgatacctgg
     1381 ttacaggggc attcgtttct ctcaaccgta gcaaaatata gtaatgatct tcctcaggct
     1441 gaaatgacag ccagagagct tgcttataac gcaccaggaa atcagggact gcgcattgat
     1501 tacgcgagtg tgttacaagc ccgcggttgg cctcgtgcag cagaaaatga attaaaaaaa
     1561 gcagaagtga tcgagccacg taatattaat ctggaggttg aacaagcctg gacagcatta
     1621 acgttacaag aatggcagca ggcagctgtc ttaacgcacg atgttgtcga acgtgaaccg
     1681 caagatcccg gcgttgtacg attaaaacgt gcggttgatg tacataatct tgcagagctt
     1741 cgtatcgctg gctcaacagg aattgatgcc gaaggcccgg atagtggtaa acatgatgtc
     1801 gacttaacca ccatcgttta ttcaccaccg ctgaaggata actggcgcgg ttttgctgga
     1861 ttcggttatg ccgatggaca atttagcgaa ggaaaaggga ttgttcgcga ctggcttgcg
     1921 ggtgttgagt ggcggtcacg taatatctgg ctcgaggcag agtacgctga acgcgttttc
     1981 aatcatgagc ataaacccgg cgcgcgcctg tctggctggt atgattttaa tgataactgg
     2041 cgtattggtt cgcaactgga acgcctctct caccgcgttc cattacgggc aatgaaaaat
     2101 ggtgttacag gcaacagtgc tcaggcttat gttcgctggt atcaaaatga gcggcgtaag
     2161 tacggtgtct cctgggcttt cactgatttt tccgacagta accagcgtca tgaagtctca
     2221 cttgagggtc aggaacgcat ctggtcttca ccatatttga ttgtcgattt cctacccagt
     2281 ctgtattacg aacaaaatac agaacacgat accccatact acaaccctat aaaaacgttc
     2341 gatattgttc cggcatttga ggcaagccat ttgttatggc gaagctatga aaatagctgg
     2401 gagcaaatat tcagcgcagg tgttggtgcc tcctggcaaa aacattatgg cacggatgtc
     2461 gtcacccaac tcggctacgg gcaacgcatt agttggaatg acgtgattga tgctggcgca
     2521 acgctacgct gggaaaaacg accttatgac ggtgacagag aacacaactt atacgttgaa
     2581 ttcgatatga cattcagatt ttaaggataa atatgttacg taatggaaat aaatatctcc
     2641 tgatgctggt gagtataatt atgctcaccg cgtgcattag ccagtcaaga acatcattta
     2701 taccgccaca ggatcgcgaa tctttactcg ccgagcaacc gtggccgcat aatggttttg
     2761 tagcgatttc atggcataac gttgaagacg aagctgccga ccagcgtttt atgtcagtgc
     2821 ggacatcagc actgcgtgaa caatttgcct ggctgcgcga gaacggttat caaccggtca
     2881 gtattgctca aattcgtgaa gcacatcgag gaggaaaacc gctaccggaa aaagctgtag
     2941 tgctgacttt tgatgacggc taccagagtt tttatacccg cgtcttccca attcttcagg
     3001 ccttccagtg gcctgctgta tgggcccccg tcggcagttg ggtcgatacg ccagcggata
     3061 aacaagtaaa atttggcgat gagttggtcg atcgagaata ttttgccacg tggcaacaag
     3121 tgcgagaagt tgcgcgttcc cggctcgttg agctcgcttc tcatacatgg aattctcact
     3181 acggtattca ggctaatgcc accggcagct tattgcctgt atatgtaaat cgtgcatatt
     3241 ttactgacca cgcacggtat gaaaccgcag cagaataccg ggaaagaatt cgtctggatg
     3301 ctgtaaaaat gacggaatac ctgcgtacaa aggttgaggt aaatccacac gtttttgttt
     3361 ggccttatgg cgaagcgaat ggcatagcga tagaggaatt aaaaaaactc ggttatgaca
     3421 tgttcttcac ccttgaatca ggtttggcaa atgcgtcgca attggattcc attccgcggg
     3481 tattaatcgc caataatccc tcattaaaag agtttgccca gcaaattatt accgtacagg
     3541 aaaaatcacc acaacggata atgcatatcg atcttgatta cgtttatgac gaaaacctcc
     3601 agcaaatgga tcgcaatatt gatgtgctaa ttcagcgggt gaaagatatg caaatatcaa
     3661 ccgtgtattt gcaggcattt gctgatcccg atggtgatgg gctggtcaaa gaggtctggt
     3721 ttccaaatcg tttgctacca atgaaagcag atatttttag tcgggttgcc tggcaattac
     3781 gtacccgctc aggtgtaaac atctatgcgt ggatgccggt attaagctgg gatttagatc
     3841 ccacattaac gcgagtaaaa tacttaccaa caggggagaa aaaagcacaa attcatcctg
     3901 aacaatatca ccgtctctct cctttcgatg acagagtcag agcacaagtt ggcatgttat
     3961 atgaagatct tgccggacat gctgcttttg atggcatatt gttccacgat gatgctttgc
     4021 tttcagatta tgaagatgcc agtgcaccgg ctatcacggc ttatcagcaa gcaggcttta
     4081 gcgggagtct gagcgaaatt cgacaaaacc cggagcaatt taaacagtgg gcccgcttta
     4141 aaagtcgtgc gttaactgac ttcactttag aacttagtgc gcgcgtaaaa gccattcgcg
     4201 gtccacatat taaaactgca cgaaatattt ttgcacttcc ggtaatacaa cctgaaagtg
     4261 aagcctggtt tgcacagaat tatgctgatt tcctaaaaag ctatgactgg accgctatta
     4321 tggctatgcc ttatctggaa ggtgtcgcag aaaaatcggc tgaccaatgg ttaatacaat
     4381 tgaccaatca aattaaaaac atccctcagg ctaaagacaa atctatttta gaattacagg
     4441 cacaaaactg gcagaaaaat ggtcagcatc aggctatttc ttcgcaacaa ctcgctcact
     4501 ggatgagcct attacaactg aatggagtga aaaactatgg ttattatccc gacaattttc
     4561 tgcataacca acctgaaata gaccttattc gtcctgagtt ttcaacagcc tggtatccga
     4621 aaaatgatta atcgcatcgt atcgtttttt atattatgtc tggtgttatg cataccccta
     4681 tgcgtagcgt actttcactc tggtgaactg atgatgaggt tcgttttctt ctggccgttt
     4741 tttatgtcca ttatgtggat tgttggcggc gtctatttct gggtctatcg tgaacgccac
     4801 tggccgtggg gagaaaacgc accagctccc cagttgaaag ataatccgtc tatctccatt
     4861 atcattccct gttttaatga ggagaaaaac gttgaggaaa ccatacacgc cgctttagca
     4921 cagcgttatg agaacattga agttattgcc gtaaatgacg gttcaacaga taaaacccgt
     4981 gccatcctgg atcgcatggc tgcacaaatt ccccatttgc gggtcattca tctggcgcaa
     5041 aaccagggga aagccattgc gcttaaaacc ggagctgccg cggcgaaaag tgaatatctg
     5101 gtgtgcattg atggcgatgc gttattagac cgcgatgcgg cggcatatat tgtggaaccg
     5161 atgttgtaca acccgcgtgt gggtgccgta accggtaatc ctcgtattcg aacacgttct
     5221 accctggtgg gtaaaattca ggttggcgag tattcctcaa ttattggttt gatcaagcga
     5281 acccagcgta tctatggaaa cgtatttacc gtttccggtg ttattgccgc atttcgtcgc
     5341 agcgccctgg cagaagtggg ttactggagt gacgatatga tcaccgaaga tattgatatt
     5401 agctggaagc tgcagttgaa tcagtggacg attttttacg agccacgggc actgtgctgg
     5461 atattaatgc ctgaaacgtt aaaagggctg tggaaacagc gcctgcgctg ggctcagggc
     5521 ggtgcagaag tattcctcaa aaatatgaca aggttgtggc gcaaagaaaa ctttcgaatg
     5581 tggccgctgt tttttgaata ctgcctgacg acaatatggg ccttcacctg cctggtcggt
     5641 ttcattattt acgcagtcca acttgccggt gtaccgttaa atattgaatt gacacatatc
     5701 gctgcgacac atactgccgg aatattattg tgtacgttat gtttactgca atttattgtc
     5761 agcctgatga tcgagaatcg ctatgagcat aatctgactt catcgctttt ctggattatt
     5821 tggttcccgg ttattttctg gatgctgagc ctagcaacga cattggtatc atttacacga
     5881 gtcatgttga tgcctaaaaa gcaacgcgcc cgttgggtaa gtcccgatcg cgggattctg
     5941 agaggttaat atgaacaatt taattattac gacccgacaa tcaccagtac gtttactggt
     6001 tgattatgtt gccacaacca tcttgtggac attatttgcg ttgttcatat tcttattcgc
     6061 catggatctg ctgacgggtt attactggca aagcgaggcc agaagccgac ttcagttcta
     6121 ttttttgctg gcagtggcga atgccatcgt gttaattgtc tgggcgctgt acaataagct
     6181 gcgttttcaa aaacagcagc atcatgcagc ctaccaatat acgccgcaag aatatgcaga
     6241 gagcttagca atacctgatg agctctatca gcaactacaa aaaagccaca ggatgagcgt
     6301 acacttcacc agccaggggc aaataaaaat ggttgtttca gaaaaagcgc tagtccgggc
     6361 ataaacaccc aaaacaaagc ccggttcgcc cgggctctgc accgataaca cacttaactg
     6421 taggcatgca gcgtacgttg gcaaagtgcc gaacgtacgc agtcctcttt accgaaccgg
     6481 acgatcccaa ccatttcatc ttcttcgaaa cgttccagcg cgtcacttaa tccggagcac
     6541 acgccgcgag gcaaatcgca ttgcgtgata tcaccgttga cgataaccgt cacgttctcc
     6601 ccgaggcggg ttaaaaacat tttcatttgc gcggcagtca cattctgcgc ctcgtcaaga
     6661 atgacgactg cattttcaaa ggtacgtcca cgcatatagg cgaacggcgc aatttccacc
     6721 ttccctattt ccggtcgcag gcagtactgc ataaaggaag cccctaagcg ccggaccagc
     6781 acgtcgtaga ccgggcgaaa atagggagca aacttttctg cgatatctcc aggtaagaag
     6841 ccaagatctt catcggcttg cagaactgga cgggtgacga taatcctgtc gacatcctta
     6901 tgtatcaggg cctctgccgc ttttgctgcg ctgatccagg tttttccgca cccggcttcg
     6961 cccgtggcga atatcagctg cttactctca atagccttca gatagtgcaa ttgcgcttca
     7021 tttcgcgcga ggatgggcga agtatcgcga ctgtcgcggg ccataccaat ggcttctacg
     7081 ccgcccatct gcacaagcga ggtgaccgat tcttcttcac gctgcttatg gctgcgcgaa
     7141 tcccgtctca gcacacgttt tgcctcacga cgagctttga tcactgcttt ttgtcttccc
     7201 atggagagca ccttgagttg tttgtattca tcacacgcgc cgttggcagc gcgattatgc
     7261 gcacgaacat cagagggttg gcttccttgt aagccatagt ttgcttttgg ataaaatgcc
     7321 gaaaaacggc tacgcacacc gtttgcggcg tcggtaacac gagtaaagag aggaagtgag
     7381 aaaaatacag agtgacgaag ttgctaccgg aggagaaatc tccgcaagtg gtggagcgta
     7441 gcttaacaag acattgtcca gaacaggacg ttaccatccg cgatctccat agtgactgac
     7501 tatcactgcc gggaacttcc gctgctactt aataattaca acagatcccg cattcattgc
     7561 aacaatatat tcacttatat ttaaccataa aacaccattt cagtgacatt agtttctact
     7621 ggaaagatga cagagtgatg acagatatga aaaaagctgt gtgctttcag caggattcgc
     7681 atcaggcatc tgcgcacgaa cgccggatgc ggcgtgaacg ccttatccgc ctaccgaacc
     7741 cgcaccgatt gtaggcataa gaagacgcgc aagcatcgca tcaggcatct gcgcaagaac
     7801 gccttatccg tctataaaca ttaaacccgc aataacgcgc ttccgagata atcgttcgcg
     7861 tccttcactc ccggcagcgc aaaaaaataa ccgccgccga taggtttaac gtattcctcc
     7921 agcgcttcgc cattgagcct tttttgtact gtcaggaagc ctttttccag atcgtgttgg
     7981 taacagacaa acagtagccc catatccagt tgcccggagt tggtgacgcc cagtgaataa
     8041 ctgtagccac gacgcagcat caggctggac tcactctccg ccgtgcgggg attcgccagc
     8101 cggatatggc tgtccagcgc aatcaccttc ccttccggat cgctggcgta atcgggaaca
     8161 tcatgctcat gctgcattcc cagcggcgca ccggtttgct tatcacgacc aaaaatcgtc
     8221 tgctgttctt tcagcggagt tctgtcccaa aattccactc gaaactgaat caagcgtact
     8281 gcctgatagc tgccaccgat tgtccacgct ggctcctgct gatctgccgt tacccacacc
     8341 actttttgca tcaacttatc attctggcta tcgggattgg cagtgccgtc tttgaaaccc
     8401 agcaaattaa tcggcgtctc tttgccttta ctacgcgccg cgtgatcgga aataaaccct
     8461 tcccgcttcc agcgcacgct gagcaaatct ggcgtatgtt tgatgatatc gcgcagcgca
     8521 tggatcaccg tgtcctgggt gttggcgcaa atttgtagca acacatcacc atgacataac
     8581 gccgcatcca gcgagtcgtt gggaaaacgc gtcatcttct gcaacttttt cggcatctgt
     8641 ggcgcaaggc caaagcgctc atcaaacaat gagtgaccca ccgataacgt gatggtgaga
     8701 ttatcgggcg caatgtagcc gccaagaatg ccggaatcga gtggtggcag gcgcggattt
     8761 ggcgtttctg gtgccgctcc gccctgagtc agaaaagcaa aacgctgagt caacaagcga
     8821 aacaaccgtt caagatcggg tttatcgctg gcaagcacat caaacgccac cagcatcatt
     8881 gcggcctgtt gtggcgtcag gatccctgcc tgatgctcac cataaaacgg ctgtttctca
     8941 ttgcgtgcat ccggtgaaag cgtacccggc gcactttgcg ttttttgtgc atgagcgacc
     9001 ggacaacttc ccgccagcgc cagcgcacca atgcctttca gtaaacgtcg gcgtgacggt
     9061 tcattcacgc cgtttttatc ttcatactgc ataatgctta atccaatccc agcacaccgc
     9121 gaagttgcgc cagatcttcc gccagcgcag taatcggtcc tttcagtgca ttccggtcgg
     9181 catcggtcaa tttgtcgtag gtttcaaaac cgtctttagt acggtatttc gccagaatgg
     9241 tatcgacctt tttaaagttg gcatcgactt ttgccaacag ttccgggttg gctttttgca
     9301 gttgtggacg cagcagatcg acaattttct gcgagccttc aacgttagcc tggaaatccc
     9361 acagatcggt gtggctgtag cgatcttctt caccgctgat tttgctggct gccacttcct
     9421 caatcagtcc ggctgcaccg ccgaccactt ttgaaggtgg gaaagccagt tcactgatgc
     9481 gtttttgcaa atcgaccaca tcggtataaa gctggtcagc gtactgatcc atgcctttgg
     9541 tggtgttgtc gccaaacaat gctttttcca gacggtggaa accggtgaat ttcggatcgg
     9601 cggctttttg ctcgtaatca tcttcacggg cgtcaatgct gccatccaga tcggagaaca
     9661 gttcagcaat cggttcgatg cgctcatagt gctggcgcgt cggtgcatac agtgctttcg
     9721 ctttttcgat atcgcctgct ttaatcgcgt cggtaaaggc tttggtgtcg gtcaccagct
     9781 gcgtggtttc cgccatgaca tacgctttat atgcagtaat tgcaccacca agacttaaca
     9841 gcgcatcact ttgcgccgca tccgccgttg cctcaccttt gacgatcaac ttccctttcg
     9901 ggttagtcag cagaccgcag gtcatatcgt attcgccagg ctgtaaattc gccgtcattt
     9961 tctggctaaa gccaggggcg atattttccc gctcttccac caccatcacg cctttgagga
    10021 tctcccactc cagcgccttc tggctgtggt tctgaataat gaactgtgtt ttcccggcgt
    10081 taaccgtaat ggtcatcggt tcgcactgct tatccgtcac ggtcactttg acctgcggca
    10141 catcagcggc gttagccata aaagcagaag aaaacagcgc agccacgctc aactgcaatg
    10201 cgttacggcg gaagttaatg gtcatgacga gactatccct ttaaagtatg ttgtaactaa
    10261 gcaagagttt gcgtcgtatt tgttacgcgg agcgagacgc tgtcgcccct gcgcgtggtg
    10321 gcagagcaaa tgccaccagc gccgggatga gataaataaa ccagacggcg acttcgctga
    10381 cgctcggcgc ttcctgatag ccaaaaatcc cttccatcag cgtgccaaac agcgagtgag
    10441 ttgagagcac cgcactcata tcgaaggcga tttcctgaaa gtggttccac aatccggctt
    10501 catgaaatgc gcgaatggca ccagctgcca gccctgcggc gacgaagaga ataaacaggc
    10561 tggtccattt aaaaaatgca ccaagattga ggcgaatacc gccccagtag agcaggaagc
    10621 ctagcaccac ggcagtagca agaccgagca ttgcacccag cggcggccag atcccgacat
    10681 cttgttgaaa tgccgccagc aggaaaaaga ccgactccag cccttccctt gcaacggcaa
    10741 aaaagaccat catcaccagc gcccagccat gatgatttcc acgctgcaat gcgctatcga
    10801 ctgcctgttc cagttgcact ttgacgttgc gcgacacttt gcgcatccag aaaaccatcc
    10861 aggtaaggat caccacggcg atcaccgcca cgataccttc aaacagttcc tgttcttttt
    10921 gcggaaattc gccggtggtt tcgttaatga agatacccaa gcccaggcac aacgcagcgg
    10981 caagcaacac gccaatccac atcacaccaa tccatcggcc tcgctgggta cgcttaagat
    11041 agctggcaat caaactgaca atcagcgcgg cttcaagtcc ttcgcgcaac ataatgagaa
    11101 acggaacaaa catgccaccc acccttaaac gtttttctga gtcaatctat gcaaagaaaa
    11161 gtaaatcgca gtgatagcga ttatcattac ggtaaagaaa aatacaagcg gaatgtttat
    11221 aaaatgatgt tgaaatgtaa acaaagagag gtaatgccag gggatataaa cgattattcc
    11281 aggtacaaag atgggattgt ttacaacgag ccatgtgttc ttgccggatg ccgcgtaaac
    11341 gccgtatcca gccgacaaaa ctgtccagac tcaatgcatc agagcttcca tgtaaaactg
    11401 ataagcgggt gtatcaagca atttcgtcca tccgcgatta tcaaatctaa ctttttgccc
    11461 ggaacataat aacagctcgg gtgttggtgg cgtcaaaatc gctgaagcgt tccccgaagg
    11521 ccgggacagc agggggcggc ggcgattggc cgccccctgc gcgctccctg caccattcaa
    11581 tttatatggc gaggcacatt aagggagcgc aacccgctgc tgaagccaaa caaaaccgcc
    11641 gcaggctaag tcccttagct ttcctgcaac cgtgacggcg gagccgaatg atagtgcgca
    11701 tcggcctcgg caaagcgttt ttgcatcgcc gctgacggtg ctttacccag caaactaaac
    11761 actacaatcc caatactgct gaagataaag cccggaataa tttcgtacag tcccagccag
    11821 ccgaactgtt tccagacgat aaccgtcagc gccccgatga tcatccccgc cagcgcaccg
    11881 ttgcgcgtca tgcgtgacca catcaccgag aacagcacca ccggaccaaa cgccgcgcca
    11941 aagcctgccc acgcatagct cactagcccc agcacacggt tttccgggtt tgccgccagc
    12001 gcaatggcca ccagcgccac caccagcacc atcacacgac ctacccacac cagctctttc
    12061 tggctggcat gtttacgcag aaacgctttg tataaatctt cggtaatcgc actggagcac
    12121 accagcagct ggcaacttaa ggttgacatt accgccgcca gaattgccga cagcagaatc
    12181 ccggcaatcc acgggttaaa cagaatttgc gccagttcga taaacacgcg ctcagcattc
    12241 tggtttaccg cacctgctac cgccgggtgc tcattaaagt aagcaatacc aaagaagccg
    12301 acagctaccg cccctgccag gcagaggatc atccaggtca tactgatacg acgcgcatgg
    12361 acaatgctgt ggtgagagtc tgccgccata aaacgcgcca ggatgtgcgg ttgcccgaag
    12421 taacccagcc cccaacccat cagtgagata atggcgacaa agttcagccc tttgagcata
    12481 tcaacgtttt cgatgctctt ttgtttgatc acttccagcg agtcaccaaa gccaccgaca
    12541 ctgataatga cgataaccgg cgtcaggatc agggcaaaaa tcatcaggct ggcctgtaca
    12601 gtgtcagtcc agctcaccgc gaggaaaccg ccaataaagg tgtaaaggat cgtcgccgca
    12661 gctcccgccc acagagccgt ttcgtagctc atgccaaagg tactttcaaa cagacgcgcg
    12721 cctgccacaa tgcccgaagc gcaataaatg gtgaagaaca gcaaaatcac cagcgcggag
    12781 ataatgcgca aaatgcggct tttatcttca aagcgcccgg tgaaataatc cggcaatgtt
    12841 aaggcgttat tgttgtattc ggtatgcaca cgcaaccgcc ccgccaccag cttccagttg
    12901 atccacgcgc ctaatgtcag gccaatggcg atccaacttt cggaaatccc ggaaagaaaa
    12961 accgcgcccg gcaaccccat taacagccag ccgctcatat ccgacgcacc cgccgataat
    13021 gccgtcacga atggcccaag gctacgaccg cccagaatat agtcgtcaaa gtttttcgtt
    13081 gatcgccagg cgataaaccc aatcaatatc atgccaaaga tatagacaca aaatgtcacc
    13141 aacatcggtg tgctaatagc catctaaagt ctccaaaaaa ttattatcgg caatgtcgaa
    13201 acttgccgtt atatctgcca ccggaacggg gtaacagagt ttatgtttta ccagggcgac
    13261 cgtatcctgc cggaagcgct ggttattcac aatcgattta acacaccatt tacattaaat
    13321 tttagtgctc agcgacacta tttttcatca ggttgcactc tctcacattt tttgcggttg
    13381 cacctttcaa aaatgttaac tgccgcagag aaaaagtctg agttattttt ttaatccctg
    13441 tcatatcgat ttcttttatt aacatttcat tcatttttaa gcttgctacg catgtcacat
    13501 ttaacatggt tgcacaaagt tgcaacatca tggatatttc acgataacgt taagttgcac
    13561 ctttcagaac aacaggagta atggcatggg aaccaccacc atgggggtta agctggacga
    13621 cgcgacgcgt gagcgtatta agtctgccgc gacacgtatc gatcgcacgc cacactggtt
    13681 aattaagcag gcgatttttt cttatctcga acaactggaa aacagcgata ctctgccgga
    13741 gctacctgcg ctgctttctg gcgctgccaa tgagagcgat gaagcaccga ctcaggcaga
    13801 ggaaccacac caaccattcc tcgactttgc cgagcaaatt ttgccccagt cggtttcccg
    13861 cgccgcgatc acagcggcct atcgccgccc ggaaaccgaa gcggtttcta tgctgctgga
    13921 acaagcccgc ctgccgcagc cagttgctga acaggcgcac aaactggcgt atcaactggc
    13981 cgataaactg cgtaatcaaa aaaacgccag tggtcgcgca ggtatggttc aggggttatt
    14041 gcaggagttt tcgttgtcat cgcaggaagg cgtggcgctg atgtgtctgg cggaagcgtt
    14101 gttgcgtatt cccgacaaag ccacccgtga tgcgttaatt cgcgacaaaa tcagcaacgg
    14161 taactggcag tcacacattg gtcgtagccc gtcactgttt gttaatgccg ccacctgggg
    14221 gctgctgttt actggcaaac tggtttccac ccataacgaa gccagcctct cccgctcgct
    14281 gaaccgcatt atcggtaaaa gcggtgaacc gctgatccgc aaaggtgtgg atatggcgat
    14341 gcgcctgatg ggcgagcagt tcgtcactgg cgaaaccatc gcggaagcgt tagccaatgc
    14401 ccgcaagctg gaagagaaag gtttccgtta ctcttacgat atgcttggcg aagctgcact
    14461 gactgccgca gatgcacaag cgtatatggt ttcctatcag caggcgattc acgccattgg
    14521 taaagcgtcc aatggtcgtg gcatctatga aggtccgggc atttcaatca agctgtcggc
    14581 gctgcatccg cgttacagcc gcgcccagta tgaccgggta atggaagagc tttacccacg
    14641 tctgaaatca ctcaccctgc tggcgcgtca gtacgatatt ggtatcaaca ttgacgccga
    14701 agaggccgat cgtctggaga tctccctcga tctgctggaa aaactctgtt tcgagcctga
    14761 gctggcaggc tggaacggta tcggttttgt tattcaggct tatcaaaaac gctgcccact
    14821 ggtgattgat tacctgattg atctcgccac ccgcagccgt cgccgtctga tgattcgcct
    14881 ggtgaaaggc gcgtactggg atagtgaaat caagcgtgcg cagatggacg gccttgaagg
    14941 ctatccggtt tatacccgca aggtgtatac cgacgtttca tatctcgcct gtgcgaaaaa
    15001 gctgctggcg gtgccgaatc taatctaccc gcagttcgcg acgcacaacg cccatacgct
    15061 ggcagcgatt tatcaactgg caggtcagaa ctactatccg ggtcagtacg agttccagtg
    15121 cctgcatggt atgggcgagc cactgtatga gcaggtcacc gggaaagttg ccgacggcaa
    15181 acttaaccgt ccgtgtcgta tttatgctcc ggtcggtacc catgaaacgc tgttggcgta
    15241 tctggtgcgt cgcctgctgg aaaacggtgc taacacctcg tttgttaacc gtattgccga
    15301 cacctctttg ccactggatg aactggtcgc cgatccggtc actgctgtag aaaaactggc
    15361 gcaacaggaa gggcaaactg gattaccgca tccgaaaatt cccctgccgc gcgatcttta
    15421 cggtcacggg cgcgacaact cggcagggct ggatctcgct aacgaacacc ggctggcctc
    15481 gctctcttct gccctgctca atagtgcact gcaaaaatgg caggccttgc caatgctgga
    15541 acaaccggta gcggcaggtg agatgtcgcc cgttattaac cctgcggaac cgaaagatat
    15601 tgtgggcttt gtgcgtgaag ccacgccgcg tgaggtagaa caggcgctgg aaagtgcggt
    15661 taataacgcg ccaatctggt ttgccactcc tccggttgaa cgcgcggcga ttttgcatcg
    15721 cgctgccgtg ctaatggaaa gccagatgca gcaactgatt ggtattctgg tgcgtgaagc
    15781 cggaaaaacc ttcagtaacg ccattgccga agtgcgcgaa gcggtcgatt ttctccacta
    15841 ctatgccgga caggtgcggg atgatttcgg taacgaaacc caccgtccat tagggcctgt
    15901 ggtgtgtatc agtccgtgga acttcccgct ggctattttc accgggcaga ttgctgccgc
    15961 actggcggca ggtaacagcg tgctggcaaa accggcagaa caaacgccgc tgattgccgc
    16021 gcaagggatc gccattttgc tggaagcggg tgtaccgcca ggcgtggtgc agttgctgcc
    16081 aggtcagggg gaaaccgtgg gcgcgcaact gacgggtgat gatcgcgtac gcggggtgat
    16141 gtttaccggt tcaaccgaag tcgctacgtt actgcagcgc aatatcgcca gccgcctgga
    16201 cgctcagggt cgccctattc cgctcatcgc tgaaaccggc ggcatgaacg cgatgattgt
    16261 cgattcttca gcactgaccg aacaagtagt aatagatgtg ctggcctcgg cgttcgacag
    16321 tgcgggtcag cgttgttcgg cgctgcgcgt gctgtgcctg caagatgaga ttgccgacca
    16381 cacgttgaaa atgctgcgcg gcgcaatggc cgaatgccgg atgggtaatc cgggtcgcct
    16441 gaccaccgat atcggtccgg tgattgatag cgaagcgaaa gtcaatattg agcgccatat
    16501 tcagaccatg cgtagcaaag gccgtccggt gttccaggcg gtgcgggaaa acagtgaaga
    16561 tgcccgtgaa tggcaaagcg gcacctttgt cgccccgacg ctgatcgaac tggatgactt
    16621 tgccgaattg caaaaagagg tctttggtcc ggtgctgcat gtggtgcgtt acaaccgtaa
    16681 ccagctacca gagctgatcg agcagattaa cgcttccggt tatggtctga cgcttggcgt
    16741 ccatacgcgt attgatgaaa ctatcgccca ggtcactggc tcggcccatg ttggttacct
    16801 gtatgttaac cgtaatatgg tgggcgcagt ggttggtgtg cagccgttcg gcggcgaagg
    16861 gttgtccggt accgggccga aagcaggcgg tccgctctat ctctaccgtc tgctggcgaa
    16921 tcgcccggaa agtgcgctgg cagtaacgct cgcgcgtcag gatgcagagt atccggtcga
    16981 tgcgcagttg aaagccgcat tgactcagcc gctaaatgca ctgcgggaat gggcagcaaa
    17041 tcgtccagaa ttgcaggcgt tatgtacgca atatggcgag ctggcgcagg caggaacaca
    17101 acgattgctg ccggggccga cgggtgaacg caacacctgg acgctgctgc cgcgtgagcg
    17161 cgtgttgtgt attgccgatg atgagcagga tgcgctgact cagctcgccg ccgtgctggc
    17221 ggtgggcagc caggtactgt ggccggatga cgcgctgcat cgtcagttag tgaaggcatt
    17281 gccatcggca gtcagcgaac gtattcaact ggcgaaagcg gaaaatataa ccgctcaacc
    17341 gtttgatgcg gtgatcttcc acggtgattc ggatcagctt cgcgcattgt gtgaagccgt
    17401 tgctgcgcgg gatggcgcaa ttgtttcggt gcagggtttt gcccgtggcg aaagcaatat
    17461 ccttctggaa cggctgtata tcgagcgttc gctgagtgtg aataccgctg ccgctggcgg
    17521 taacgccagc ttaatgacta taggttaaac aggtagccgg aggatgttac aacctcctcc
    17581 ggcatcttta acgtggtcga atcccctcaa taataatccg ctgcacgttt tcaaccgttt
    17641 gattgaaaaa tatctcatcg cgcaacgtcg cgcctgtcac cgcctccacc tgaggggcga
    17701 aatcggcgta atgttgagtg gaagcccaaa tcatgaaaat caaatgctgc ggatcaatcg
    17761 gcgcgagttt gccgcttttg acccaaccgg caatcagcgc cgatttctca tcaattaatg
    17821 ccttcaaatc gcccgtcagt tcatccatta acagcggcgc gcctgccagc atctccatac
    17881 agaacaggcg cgaagcctgc ggataatcac gggagacttc cagcttcaga cggatgtact
    17941 ctttgatcgc cgccagcgga gcgaaatctt cacgaaacgc ttttaacggt gccagccaga
    18001 tatcgagaat ctgccgcagc acggcaatat acagcgcctc ttttgacggg aagtaataca
    18061 gcagattggt ttttgaaaca cccgccaact ctgcgatctg ctccagcctt gtgccgtgaa
    18121 aaccgaattg tgaaaaagtg tccagtgctg cgctaagaat cgcttttttc ttcgcgctta
    18181 ctgcgcgcga acgtttaccc gttgttttca ctgcgccttg cgtcatgcgc tctcccctct
    18241 tgtagattgg caacaggata gcaaaacccc cgcccggcct cgaccttctg cactctcatc
    18301 gcgcacagat gcatgtttta tgtgcaactg ttttgaccgt ttagtccact ttttaccaga
    18361 tattttagat aacgcattgt taacacatta acaacattaa ctttttaaaa actggcatcc
    18421 gctttgcaaa caagccaata cgcggctgaa aaggatatgc aggatgcagc gccccgtctg
    18481 actttcactt tacgagacga agagaggttg atgatgaaaa ttggcgtatt cgtacctatt
    18541 ggcaacaacg gctggctcat ttcgacccac gcgccgcagt acatgccgac ctttgaactg
    18601 aataaagcca ttgtgcaaaa agcggagcac taccatttcg atttcgccct gtcgatgatc
    18661 aaactgcgtg gctttggcgg caaaactgag ttctgggatc acaaccttga gtcgttcacc
    18721 ttgatggcgg ggctggcagc cgtgacctcg cgcattcaga tttacgcgac agctgccacc
    18781 ttaacgttac ctccggcaat cgtcgcccgt atggccgcaa ccatcgactc catctctggc
    18841 gggcgttttg gcgtcaacct cgtgactggc tggcaaaagc ccgagtatga gcagatgggt
    18901 atctggcctg gcgatgacta tttctcccgt cgttacgact atctcaccga atatgttcag
    18961 gtgctgcgcg acctgtgggg cacggggaaa agcgatttta aaggcgattt tttcaccatg
    19021 aatgattgtc gcgtcagtcc gcaaccgagt gtccccatga aagtgatctg cgccgggcaa
    19081 agcgacgctg gcatggcgtt ctccgcccag tatgccgatt tcaacttctg tttcggcaaa
    19141 ggtgtaaata cacccacggc tttcgccccg accgctgtgc ggatgaaaca ggccgcagag
    19201 caaaccgggc gcgacgttgg ctcttatgta ttgtttatgg tgattgccga tgaaaccgac
    19261 gatgccgctc gcgccaaatg ggaacactac aaagcgggcg cggatgaaga ggcgttaagc
    19321 tggctaaccg aacaaagtca gaaagatacc cgctccggta ctgataccaa cgtccgtcag
    19381 atggccgatc ccacttcggc ggtaaacatc aatatgggga cgttagtcgg ttcttacgcc
    19441 agtgtcgcgc gcatgttaga tgaagtcgca agcgtgcctg gtgccgaagg cgtgctgtta
    19501 accttcgatg attttctgtc gggaatcgaa accttcggcg agcgcattca accactgatg
    19561 cagtgccgcg cccatctccc tgcgctgact caggaggtgg catgatgacg accttaaccg
    19621 ctcgaccgga agccattacc ttcgatccgc agcaaagtgc gctgatcgtg gtggatatgc
    19681 aaaacgctta tgccacgcca ggcggctact tagatctcgc cgggtttgat gtctcaacca
    19741 ctcgcccggt cattgccaac attcaaaccg ccgtgaccgc agcgcgagcg gcagggatgc
    19801 tgatcatctg gtttcaaaat ggctgggatg aacagtatgt cgaagctggc ggacccggct
    19861 caccgaattt tcataaatcg aacgccctga aaaccatgcg taagcagccg cagctgcagg
    19921 ggaaattgct ggcgaaaggc tcctgggatt atcaactggt ggatgaactg gtgccgcagc
    19981 ctggcgatat tgtgctgccg aagccgcgct acagcggttt cttcaatacg ccgctggaca
    20041 gcattttgcg cagccgtgga atacgccatc tggttttcac cggtatcgct accaacgtct
    20101 gcgtcgaatc gacgctacgc gatggctttt ttctggagta tttcggcgtg gtgctggaag
    20161 acgcaacaca ccaggcgggg cctgaatttg cacagaaagc cgcgttgttc aatatcgaaa
    20221 ccttttttgg ctgggtcagc gacgtcgaaa cattctgcga cgcgctttct cccacgtcct
    20281 ttgctcatat cgcttaagga gtttaacgat gccaaaatcc gtaattattc ccgctggcag
    20341 cagcgcaccg ctggccccct tcgttcccgg cacgctggct gatggcgtgg tgtatgtctc
    20401 cggtacgctg gcttttgatc aacataataa cgtgctgttt gccgatgacc caaaggcgca
    20461 aacccgccac gttctggaaa ctatccgcaa ggtgatcgag acggcgggtg gcacgatggc
    20521 ggatgtgacc ttcaacagca tctttattac cgactggaaa aattacgccg cgattaacga
    20581 aatctacgcc gagttttttc cgggtgataa accggcgcga ttctgcattc agtgcggact
    20641 ggtaaaacct gacgcgctgg tggaaatcgc cacaattgcg catatcgcca agtgaggccg
    20701 cgatgaaact ttcactctca cctccccctt atgctgatgc gcccgtagtg gtgttgattt
    20761 cgggtcttgg gggtagcggc agttactggt taccgcaact ggcggtgctg gagcaggagt
    20821 atcaggtagt ctgttacgac cagcgcggca ccggcaataa tcccgacacg ctggcagaag
    20881 attacagtat cgcccagatg gcagcggaac tgcatcaggc gctggtagcc gcagggattg
    20941 agcattacgc agtggtcggc catgcgctcg gtgcgctggt gggaatgcag ctggcgctgg
    21001 attatcccgc gtcggtaact gtgctgatca gcgttaacgg ctggctacga ataaacgccc
    21061 atacgcgccg ctgttttcag gttcgcgaac gattactgta tagcggcggc gcgcaggcat
    21121 gggtggaagc gcagccgttg ttcctctatc ccgccgactg gatggcggcc cgcgcacctc
    21181 gcctggaggc agaagacgcg ctggcactgg cgcattttca gggcaaaaat aatttactgc
    21241 gtcgacttaa cgccctcaaa cgcgctgact ttagtcacca tgcggatcgc atccgctgcc
    21301 cggtgcaaat catctgcgcc agtgatgatc tgctggtgcc aacagcatgt tccagtgaac
    21361 ttcatgccgc cctgcccgat agccagaaaa tggtgatgcc ctatggcgga cacgcctgca
    21421 acgtgaccga tcccgaaacg tttaatgctc tgttactcaa cgggcttgcc agcctgttac
    21481 atcaccgtga agccgccctg taaggaattg ctatgaacga agccgttagc ccaggtgcgc
    21541 ttagcaccct gttcaccgat gcccgcactc acaacggctg gcgggagaca cccgtcagcg
    21601 atgagacgtt acgggagatt tatgccctga tgaaatgggg gccgacatca gctaactgtt
    21661 ctccggcacg gatcgtgttt atccgcacgg cagaaggaaa agaacgtctg cgcccggcac
    21721 tttccagcgg caatctgcaa aaaaccctga ccgcgcccgt caccgctatc gtcgcctggg
    21781 acagtgaatt ttatgaacgg ttaccactac tgtttcccca cggtgatgcc cgcagttggt
    21841 ttacctccag cccacaactt gccgaagaaa cagcgtttcg caacagttcc atgcaggcgg
    21901 cctatctgat cgtcgcctgc cgggcgctgg gactggatac cggcccgatg tcgggctttg
    21961 accgtcaaca cgtggacgac gcctttttta cgggcagcac gctgaagagc aatctgctga
    22021 ttaatatcgg ctatggcgat agcagcaagc tttatgcgcg cctgccacgt ctgtcctttg
    22081 aagaagcctg cgggctgttg taaggagcaa actatgaaca ttgtcgatca acaaactttt
    22141 cgcgatgcga tgtcctgcat gggcgcggcg gtcaatatta tcaccacgga cggtccagcc
    22201 gggcgcgccg ggttcaccgc cagcgccgtc tgcagtgtga ccgatacgcc gcccacatta
    22261 ctggtgtgcc tgaatcgtgg ggcgtccgtc tggccggcat tcaatgaaaa tcgaacgctg
    22321 tgtgtaaata cacttagcgc cgggcaggag ccgctttcaa acctttttgg cggcaaaacg
    22381 cccatggaac accgctttgc cgccgcccgc tggcagacgg gggtgaccgg atgcccgcaa
    22441 ctggaagagg ctctggtttc gtttgactgc cgtatcagcc aggtggtgag cgtcggcacc
    22501 cacgacattc tgttttgcgc catcgaagcg attcatcgtc acaccacacc ctacgggctg
    22561 gtgtggttcg atcgcagtta tcacgcgctg atgcgccctg cttgttaacc tccttaagga
    22621 gacagctgat ggcaatgttc ggttttcctc actggcagtt aaaatcgacc tctacagaga
    22681 gcggcgtggt cgcgccggat gaacgattgc cgtttgctca gacggcagta atgggcgttc
    22741 aacacgcggt ggcgatgttt ggcgcgacgg tgttaatgcc gattctgatg gggctggatc
    22801 ccaatctttc cattttaatg tcggggatcg gcacgttact gtttttcttt attaccggcg
    22861 ggcgcgtccc cagttatctc ggctccagcg cggcttttgt cggcgtggtg atcgccgcga
    22921 cgggctttaa cggtcagggc attaacccga atatcagcat tgcgctgggc gggattatcg
    22981 cctgtgggct ggtttatacc gtcattggcc tggtggtgat gaaaatcggt acgcgctgga
    23041 ttgaacgact gatgccgccg gtggtgacgg gtgcggtggt gatggcgatt ggcctgaact
    23101 tagcgcctat cgcagtgaaa agtgtatcgg cttcggcctt tgatagctgg atggcggtaa
    23161 tgacggtgct gtgtattggg ctggtggcgg tgtttacccg tggcatgatc cagcggttat
    23221 tgattctcgt aggattaatt gtcgcctgtc tgctgtatgg cgtgatgacc aacgttttag
    23281 ggctgggaaa agccgtcgat tatactctcg tcagccatgc tgcctggttc ggtctgccac
    23341 atttttccac tcctgctttt aatggtcagg caatgatgct gattgcgccc gtggcggtga
    23401 ttctggtggc ggaaaactta ggtcatctca aggcagtcgc cgggatgacc ggacgcaata
    23461 tggatccata tatgggacgg gcattcgtcg gtgatgggct ggcgacgatg ctttctggtt
    23521 ctgtcggtgg cagcggcgta accacctacg ccgagaatat cggtgtaatg gccgtgacaa
    23581 aagtctactc gacgctggtg tttgtcgctg ctgccgtcat cgccatgctg ttgggctttt
    23641 caccgaaatt tggcgcgctg attcatacca ttccggccgc ggtgattggt ggtgcatcaa
    23701 ttgttgtgtt cgggctgatt gccgtcgcag gcgcaaggat ctgggtacaa aaccgtgtcg
    23761 atttaagcca gaacggtaat ttgattatgg tcgcagtaac cttagtgctg ggcgcgggtg
    23821 attttgctct cacgctgggc ggttttacat tgggagggat tggtacagca acctttggcg
    23881 cgattttact caatgcgtta ttaagcagaa ggttggttga cgttccaccg ccagaagttg
    23941 ttcatcagga gccataaaaa gaaaaccgcc gggattaccc ggcggtgcag attgttgaca
    24001 acgtacgcat tgttcatgcc ggatgcggcg taaacgcctt atccggccta caaaacagtg
    24061 caaattcaat atattgcaat cttctggtag gcctgataag catcgcgcat caggcaagct
    24121 tacatttgtc atcagtatca accaccgggt aaccggcggt gatggctttt tgacgagcta
    24181 cagacaaaaa tgcattcgtc actgattagt ccggctagtt gtcgcttttg ccgtgactgc
    24241 tcttgccacc ttttttacct gcttcagatg cgcgctgcgg gtcattcttg aaattacccc
    24301 cgctgtgctg tccacctttt ttacctgctt ctgatgctct ttcgcggtct tctgcaaaat
    24361 tgccggaacc gcctcgatgg tttgccatta ctgacctccg tctgggtata gacatcatat
    24421 tgcatagaat taacgcggcg tatggcggct gccatatttt cgctacgtgg gactaagctt
    24481 agtgaactga cgcgggcaga cccgcgtttg gtaatatcct gcaacagctt cttagcgaga
    24541 atagatatca tctccaataa ttatccataa gccgctctta tgattaaaaa aacgcgatga
    24601 gataaaaatg tgtcattttg cgacaaaatt acgtgcttgt gaaagttgtt ataaatcaaa
    24661 taagtggttg tgaaatttgc actctgaaaa ggacgtctta tctttaaata agtggtagcg
    24721 aatcgctacg gaatagagat aacacgagga gtggttagaa atggctaaag ttctggtgct
    24781 ttattattcc atgtacggac atattgaaac gatggcacgc gcagtcgctg agggtgcaag
    24841 caaagtggat ggcgcagaag ttgtcgttaa gcgtgtaccg gaaaccatgc cgccgcaatt
    24901 atttgaaaaa gcaggcggta aaacgcaaac tgcaccggtt gcaatcccgc aagaactggc
    24961 cgattacgac gccattattt ttggtacacc tacccgcttt ggcaacatgt ccggtcaaat
    25021 gcgtaccttc ctcgaccaga cgggcggcct gtgggcttcc ggcgcactat acggaaaact
    25081 ggcgagcgtc tttagttcca ccggtactgg cggcggtcag gaacaaacta ttacttcaac
    25141 ctggacgacc cttgcgcatc acggcatggt aattgtcccc attggctacg cagcgcagga
    25201 attatttgac gtttcacagg ttcgcggcgg tacgccgtac ggcgcaacca ccatcgcagg
    25261 cggtgacggc tcacgccagc caagccagga agaactgtct attgctcgtt atcaagggga
    25321 atatgtcgca ggtctggcag ttaaacttaa cggctaatct tcaacaggag gatacgcatg
    25381 ccaactcaag aagcgaaagc tcatcacgtc ggtgaatggg catctctgcg caatacgtcg
    25441 ccggaaatag ccgaggccat tttcgaagtc gccgggtacg acgaaaaaat ggcggaaaag
    25501 atttgggaag aaggtagcga tgaagtgtta gtcaaagcct ttgctaagac cgataaagat
    25561 tcgctttttt ggggcgaaca gaccatcgaa cgtaaaaacg tttaaccagc gactcccccg
    25621 cttctcgcgg gggagttttc tgttatttca ccgcttcatt caacacgcta tcaaatttat
    25681 ccatcgggca gaaaccgttg gcatctatcg ggcaaccgct taattccagc gtcacacgct
    25741 gagcaggtgc ctgcagggtt aacgcatcgg cattacgtaa ctgttccgca ctctgataca
    25801 catattcaat tttcatcaaa tcgcgattgg ctttgctgtc atgccaacgc tggaaaacga
    25861 ttttgccgcc aatcggcgtg cgttcgttct ggtcatgcaa ctgatacggt ttgaaatcca
    25921 gcgccgttaa cagagaggca atgttggagt cgtgcccaac caacactgta attttcggtg
    25981 cgctggtgcg atcggtgacc agagctttgt cgatataact gaccagcggt ttcgcaacat
    26041 tgcgcgccac ttccggtgag gtaaacaggc tgtcctggta gccgttttta agcttcgaca
    26101 acaccttcca ctgctggtca gatttgattt ctccccaggc cacctgatcc atcggaaaac
    26161 cttcgtaata ttgcaaagta aacgcatcta ccagcgagtt gccgactttc agcggcccgg
    26221 aaacacctgg ttcttgttga tacttggcgc taaaggtatt tttgccatcc accagcgaac
    26281 actgttgttt ctctttacag gcaggggaat ctttatagtt aacgattttt tccagtagct
    26341 ggtagctgtc ggtaagctgg agtttgctga gctctttctc cattgccgcc accgcctgtt
    26401 cactgaatgc ggcggaatca tcggtgatca ccgggttaaa ggttgggtcc atagtgccca
    26461 ttttttcctg gtgatgcaca ggaatatcac accccgggaa tgcgccggta ataaagaact
    26521 gtgcggtcgc aacggtacgt tgcagactat tggcataggc ataaacggtg tacggcggcg
    26581 ggcattcccc cgatttcacc atcccctgct ctgccagcca ttcacgcatg taatggccca
    26641 tatacacttc gagcacgcca cctttggtgg tgagttgccc accggggacg tcccattctg
    26701 gccatttatt cggcgtcgac tgctccagca cactgccatt gttcgccagc ggcgcacgta
    26761 agttatggcg gctcatcatg agcacttgct gtagctgata gccttccggt acggtttgtg
    26821 cctgagcgtt tgaagcgagt aaaactatcc ctgccacagc tgcggcgatt agcgttttgt
    26881 tcattcctgg cacctctttt gttatcaatc actaaagagt gtgacagaaa tatgactttt
    26941 ttccgggaac aggttcgcaa aatggcttgt tgaacgcctg tttttctata tacatcttca
    27001 aaaacaggca aggtttgacg caaactatgc ctgattagcc cctgcctcgc gtacgccgct
    27061 gataattgca cctgtggcta aacagaatat ctgcggcgtt accaatacag agacaatatt
    27121 taccaataaa aaagtgtgag actaataaca atggttttta gcatatgcct ttgccattaa
    27181 attgcataac gatttaaata gtcgagttgc gcattcattt ccttattttt taacttgtaa
    27241 gcctgagcaa aaagttcagg agggaggtaa acatcataat gattgccagt gtttattaaa
    27301 taaccatact caagatcttc atctgttgaa aaggattcat tccctaagga gattgatgcc
    27361 acattactat taacaataaa taatgagcaa atgctatctg aaagagcagt gaggaaattt
    27421 ttcacctcat taatcctctt ctctgggtcg gtgtctaaaa
//
LOCUS       DAPHBJ010000045        27342 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.045, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000045 DAPHBJ010000000
VERSION     DAPHBJ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.045"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(95..3244)
                     /locus_tag="RHZ76_001697"
     CDS             complement(95..3244)
                     /locus_tag="RHZ76_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDV2812350.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3267..4460)
                     /gene="acrA"
                     /locus_tag="RHZ76_001698"
     CDS             complement(3267..4460)
                     /gene="acrA"
                     /locus_tag="RHZ76_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HDV2812351.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4602..5249
                     /gene="acrR"
                     /locus_tag="RHZ76_001699"
     CDS             4602..5249
                     /gene="acrR"
                     /locus_tag="RHZ76_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HDV2812352.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5377..8739
                     /gene="mscK"
                     /locus_tag="RHZ76_001700"
     CDS             5377..8739
                     /gene="mscK"
                     /locus_tag="RHZ76_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDV2812353.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWQKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLAGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLVVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8951..9112)
                     /gene="rsmS"
                     /locus_tag="RHZ76_001701"
     CDS             complement(8951..9112)
                     /gene="rsmS"
                     /locus_tag="RHZ76_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HDV2812354.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9126..9653)
                     /gene="priC"
                     /locus_tag="RHZ76_001702"
     CDS             complement(9126..9653)
                     /gene="priC"
                     /locus_tag="RHZ76_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HDV2812355.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLVRVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9723..10100
                     /locus_tag="RHZ76_001703"
     CDS             9723..10100
                     /locus_tag="RHZ76_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HDV2812356.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10253..10804
                     /gene="apt"
                     /locus_tag="RHZ76_001704"
     CDS             10253..10804
                     /gene="apt"
                     /locus_tag="RHZ76_001704"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDV2812357.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10933..12864
                     /gene="dnaX"
                     /locus_tag="RHZ76_001705"
     CDS             10933..12864
                     /gene="dnaX"
                     /locus_tag="RHZ76_001705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDV2812358.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRTSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEDSIRPI"
     misc_feature    12197..12261
                     /gene="dnaX"
                     /locus_tag="RHZ76_001705"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12917..13246
                     /locus_tag="RHZ76_001706"
     CDS             12917..13246
                     /locus_tag="RHZ76_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDV2812359.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13246..13851
                     /gene="recR"
                     /locus_tag="RHZ76_001707"
     CDS             13246..13851
                     /gene="recR"
                     /locus_tag="RHZ76_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDV2812360.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13961..15835
                     /gene="htpG"
                     /locus_tag="RHZ76_001708"
     CDS             13961..15835
                     /gene="htpG"
                     /locus_tag="RHZ76_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDV2812361.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16016..16660
                     /gene="adk"
                     /locus_tag="RHZ76_001709"
     CDS             16016..16660
                     /gene="adk"
                     /locus_tag="RHZ76_001709"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDV2812362.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16792..17754
                     /gene="hemH"
                     /locus_tag="RHZ76_001710"
     CDS             16792..17754
                     /gene="hemH"
                     /locus_tag="RHZ76_001710"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDV2812363.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17751..18710)
                     /gene="aes"
                     /locus_tag="RHZ76_001711"
     CDS             complement(17751..18710)
                     /gene="aes"
                     /locus_tag="RHZ76_001711"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HDV2812364.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18862..20166
                     /gene="gsk"
                     /locus_tag="RHZ76_001712"
     CDS             18862..20166
                     /gene="gsk"
                     /locus_tag="RHZ76_001712"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HDV2812365.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20299..21975)
                     /locus_tag="RHZ76_001713"
     CDS             complement(20299..21975)
                     /locus_tag="RHZ76_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HDV2812366.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22213..23433)
                     /gene="fsr"
                     /locus_tag="RHZ76_001714"
     CDS             complement(22213..23433)
                     /gene="fsr"
                     /locus_tag="RHZ76_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HDV2812367.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23651..25303
                     /gene="ushA"
                     /locus_tag="RHZ76_001715"
     CDS             23651..25303
                     /gene="ushA"
                     /locus_tag="RHZ76_001715"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HDV2812368.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25340..25819)
                     /gene="ybaK"
                     /locus_tag="RHZ76_001716"
     CDS             complement(25340..25819)
                     /gene="ybaK"
                     /locus_tag="RHZ76_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HDV2812369.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(26023..26817)
                     /locus_tag="RHZ76_001717"
     CDS             complement(26023..26817)
                     /locus_tag="RHZ76_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HDV2812370.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            26955..27296
                     /locus_tag="RHZ76_001718"
     CDS             26955..27296
                     /locus_tag="RHZ76_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDV2812371.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERTKKVA"
BASE COUNT         6786 a         7129 c         7176 g         6251 t
ORIGIN      
        1 tgagatcctg agttggtggt tcaattactc cttaatgttc gtaggttatg cataaaaaag
       61 gccgcttacg cggccttagt gattacacgt tgtatcaatg atgatcgaca gtatggctgt
      121 gctcgatatc ttcattcttg cggctaaagc ggcggcgaac caccacaaag aataccggaa
      181 cgaagaagat tgccagtacc gttgcggtca ccatcccgcc cattacaccg gtacctactg
      241 cgttctgcgc gccggaacca gcaccagtac tgataaccag cggcataacg ccgaggataa
      301 acgccagcga ggtcatcagg atcggacgta aacgcatccg caccgcatca agcgtcgctt
      361 caatcagacc tttaccttct ttatccatca agtctttggc gaattcgacg ataaggatcg
      421 cgttcttcgc cgacaaccca atggttgtga gcaggcctac ctggaagtaa acgtcattgg
      481 tcaggccacg gaaggtggca gccagtaacg caccgataac ccccagcgga acgaccagca
      541 taacggagaa cggaatcgac cagctctcgt acagcgccgc cagacacagg aacacgacaa
      601 tcaacgaaat cgcgtacagt gaaggtgcct ggttgccgga gagacgttcc tgataggaca
      661 tccccgtcca gtcatagcca acaccggtag gcagtttgct cgccagttgt tccatcagct
      721 ccattgcttc accggtactt ttacccggtg ccgcctggcc taagatttcc atggatggca
      781 ggccgttgta acgttccaga cgcggcgaac cgtactccca acgagaagag gagaacgccg
      841 agaatggcac catctgacca tcagcagcac gaacatacca gtcgccgata tcatccggca
      901 gcatacggta tttcgcttct gacatgacat aaactttctt cacacgaccg cggtcgataa
      961 agtcgttcac atagctgccg ccccatgcag cgcccagagt ggtgttaatg tcgttgatag
     1021 aaacacccag cgcctgcgct ttttcctggt cgatatcaat cttaaactgc ggggtatctt
     1081 ccagaccgtt tggacgtacg ctggtcaaca tatcagggtg cttcgctgct tctgcaagca
     1141 actggttacg cgcctgagtc agtttttcgt gaccaaggcc agcctggtca atcagctcaa
     1201 agtcaaagcc ggttgcagta cccagttcca cgattgcggg caggttaaag gcgaaaacca
     1261 tcgcatcttt gatttgcgag aaagcgcgtg ttgcacgcat ggtaatcgct tcaactttgt
     1321 tttcttcgcc cggacgatcg gcccagtcct tcaaggaaac gaacgcaata ccggtattct
     1381 gaccacgtcc cgcaaagccg aagccgttaa cggcgaacac cgactcaacg ttgttctttt
     1441 ctttggtcag atagtaatgc gttacctcat tgagcacttt ctgtgtacgt tcctgcgttg
     1501 cacctgctgg cagctgaacc atggtcataa acacgccctg gtcctcatct ggcaagaagg
     1561 agcttggcag acgcacgaac agataggcca tgccgaccac gatgatcaga tacagcacca
     1621 ggtaacgccc cgtactgcgc agaataccgc ctacgctgtc ggtgtagtgg tgcgtgctct
     1681 tctcgaacat gcggttaaac cagccgaaga agcctttttt accttccccg tgatcgcctt
     1741 tggcaatcgg tttcagcatg gtggcacaaa gagctggagt caggatcaac gccaccagta
     1801 ccgacagcgc cattgctgaa acaatggtaa tagagaactg acgatagata gcaccagtag
     1861 aaccgccaaa gaaggccatc ggtacgaata ccgccgacag taccatcgcg ataccgacca
     1921 gagcgccctg aatctgcccc atcgacttac gggtagcttc ttttggcggc aaaccttctt
     1981 ccgccataac acgctcaacg ttttctacca caacgatggc gtcatccacc aacaggccga
     2041 tggcgagcac catcccgaac attgttagtg tgtttatcga gaaaccaaag gcggcaagga
     2101 cggcaaaggt cccgagcaat accaccggta cggcaatggt cggaatcaac gtcgcgcgga
     2161 agttctgcag gaacagatac ataaccagga acacgaggat gatcgcttcg accagcgttt
     2221 taaccacttc gtgaatagag attttcacga acggcgtggt gtcgtatggg taaacaattt
     2281 tcagacccga cgggaagaac ggttccatct tcgccagttc agcacggatt gccgcagcgg
     2341 tatccagcgc gtttgcaccg gtcgccagct tgatccccag accggaagcc ggttggccgt
     2401 taaactctgc gatgatgtcg tagttctcac cacccagctc aatcttcgcg acgtcacgca
     2461 gcagcacgcg ggaaccatcc tgattcactt tcagcaggat tttgccgaac tcttcagtag
     2521 aggtcagacg cgtctgagca ataatagagg cgttaagctg ttggcctttc accggcggcg
     2581 taccaccgag ctgacccgcc gcaacctggg cgttctgcgc tttgatggcg gtaatgacat
     2641 caaccggcgt tagctggaat ttgttcagct cattcgggtt catccagata cgcatcgcgt
     2701 actgtgaacc gaacaactga acatcaccca cgcccgacgt acggctgatg gcatctttca
     2761 tattcgccgc cacgtagtcg gagatatcct cctgcgtcat ggtgccatcg gtgttgataa
     2821 cgccgacaac catcaggaag ctgctggatg atttctcaac gctcacccct tgctgctgaa
     2881 cttcttgcgg cagcaacggc atcgccagct gcagtttgtt ctgcacctga acctgcgcga
     2941 tatccgcatc agtaccagac tcaaaggtca gggtgatctg cacggtaccc gtggagtcac
     3001 tgttagagga catgtacatc aggttatcga taccgttcat attctgttcg ataacctgtg
     3061 tcaccgtgtc ctgcactgtt ttcgcatcag cgccggggta ggaggcggag atcgttactg
     3121 ccggcggtgc aatcgtagga tattgcgcca ccggcagttt gaggatcgcc agcccccctg
     3181 ccaacatgat gataatggcg atcacccacg caaaaatcgg gcgatcgata aagaaattag
     3241 gcatgtctta acggctcctg tttaagttaa gacttggact gttcaggctg agcaccgctt
     3301 gcggcttgct ggttattatc agcggtaact tcttgtgctt ttacctggac accaggacgc
     3361 actttctgca gcccacttat tactacgcga tcgcctgctt tcagaccttc tgtcaccagc
     3421 cacttatcgc caatagcctg gcttgcaacg atcggacggg tttccacttt gtcatccgcg
     3481 ccaactacca gtacggtggc atcgccacgc ggcgtacggg ttacgccctg ttgcgggact
     3541 aaaatagcgt ttggattaag cccttcttcc agacgtgcgc gcacgaacat acccggcagc
     3601 agagtgtgat ccgggttcgg gaagatagcg cgtagggtga tagacccagt ggtctgatca
     3661 acggtaacgt cagagaattc cagcgtaccg tcctgcggga acttaatgcc gtcactggtg
     3721 atcagtgaca ctttggcttt gccgttctct tgtttcagcg tgccattcgc cagttcctgt
     3781 ttcaggcgca ggaagtcgtt gctggactgg gtcacatcaa cgtagatcgg atcaagttgc
     3841 tgcacggttg ccagcgcagt cgcctgaccg ttctgtacca atgcgccttc cgtcacgttc
     3901 gacttaccaa tgcgaccgct aatcggagag gtgactttgg tgtaagccag attgatccgc
     3961 gcagtttcaa cggcagcttt cgccgcagtt accgcagcat tcgcctgttg cgcatcagcc
     4021 agagcctgat cgtactcttg cttactgatg tactgagtac cgagcagttt ctgataacga
     4081 ttcaccgtca attgcgcgat attggctgca gcctgggctt tcgccagatc acctttcgca
     4141 ctgtcgtatg tcgcctgata ggtcgcagga tcaatctgat agagagagac acctgcttcg
     4201 atgtcgctac cttctttgaa attacgcttc aggataatcc cgctaacttg aggacgaact
     4261 tctgcgatcc ggtaggcact ggtgcgaccc ggaagctcgg ttgtgatctg cagaggttca
     4321 gttttgactg ttactacgcc aacggcgggc atctgctggc caccttgttg ggcctgtttg
     4381 tcgtcacatc ctgttagggc taagctgcct gagagcatca gaacgaccgc cagaggcgta
     4441 aaccctctgt ttttgttcat atgtaaacct cgagtgtccg atttcaaatt ggtcaatggt
     4501 caaaagttaa taaacccatt gctgcgttta tattatcgtc gtgctatggt acatacattc
     4561 acaaatgtat gtaaatctaa cgcctgtaaa ttcacgaaca tatggcacga aaaaccaaac
     4621 aagaagcgca agaaacgcgc caacacatcc tcgatgtggc tctacgtctt ttctcacagc
     4681 agggggtatc atccacctcg ctgggcgaga ttgcaaaagc agctggcgtt acgcgcggtg
     4741 caatctactg gcattttaaa gacaagtcgg atttgttcag tgagatctgg gaactgtcag
     4801 aatccaatat tggtgaacta gagcttgagt atcaggcaaa attccctggc gatccactct
     4861 cagtattaag agagatatta attcatgttc ttgaatccac ggtgacagaa gaacggcgtc
     4921 gattattgat ggagattata ttccacaaat gcgaatttgt cggagaaatg gctgttgtgc
     4981 aacaggcaca acgtaatctc tgtctggaaa gttatgaccg tatagaacaa acgttaaaac
     5041 attgtattga agcgaaaatg ttgcctgcgg atttaatgac gcgtcgcgca gcaattatta
     5101 tgcgcggcta tatttccggc ctgatggaaa actggctctt tgccccgcaa tcttttgatc
     5161 ttaaaaaaga agcccgcgat tacgttgcca tcttactgga gatgtatctc ctgtgcccca
     5221 cgcttcgtaa tcctgccact aacgaataac cctgaatctg actccaggat ttttcctgga
     5281 cattttcgtc gttgctattc tggttcactg cgtcgtgata ttcttgcggt ttgacttttt
     5341 caggtcgttc ttcaggttca gaaaccttca ttcatcatga ctatgttcca gtattacaaa
     5401 cgatcacggc attttgtttt ttcagcattt attgcttttg tttttgtctt gttatgccag
     5461 aacacggcgt ttgcgcgggc gtcatcgaat ggtgatctgc cgacaaaagc ggacctgcag
     5521 gcgcaacttg actcactaaa taaacaaaaa gatctttctg ctcaggacaa actggtgcag
     5581 caggatctga cagatacatt agccaccctc gataaaatcg atcgcataaa agaagagaca
     5641 gttcagctac ggcaaaaagt cgctgaagcg ccggaaaaaa tgcgccaggc gaccgcggcg
     5701 ttaacagcac ttagcgatgt cgataacgac gaagaaacgc gcaaaattct gagcacgctt
     5761 tcgttgcgcc agctggaaac tcgcgttgcc caggcgctgg acgatttgca aaacgcacaa
     5821 aacgatctgg cgtcttataa cagccagctg gtttcgttac agacgcagcc cgaacgcgtg
     5881 caaaatgcga tgtataacgc ttcgcagcag ctgcaacaaa ttcgcagtcg tctggatggg
     5941 actgatgtcg gcgagacagc cttacgtccc agccagaaag tgttaatgca ggcccagcag
     6001 gcgttgctga atgcggagat tgaccagcag cgtaaaagcc tggaagggaa caccgtcttg
     6061 caggatacct tgcaaaagca acgtgattac gtgacggcga acagcgctcg tctggagcac
     6121 cagttacaac tgttgcaaga agcggtaaac agcaagcgcc tgactttaac cgaaaaaacg
     6181 gcgcaggaag ccgtctcccc ggatgaagcc gcgcgtattc aggctaatcc gctggtgaag
     6241 caggaactgg aaattaacca gcagttaagt cagcgtctga ttaccgcgac tgaaaacggt
     6301 aatcagttga tgcagcaaaa cattaaagtc aaaaactggc tggagcgggc gctgcaatcg
     6361 gaacgcaata ttaaagagca gattgccgtc ctgaagggca gcctgctgtt gtctcgtatc
     6421 ctttaccagc aacaacaaac gctgccctcg gcggatgaac tggaaaacat gaccaaccgc
     6481 atcgcggatt tgcgtctcga acagtttgaa gttaaccagc agcgtgatgc actcttccag
     6541 agcgatgcgt tcgtcaacaa actggaagaa ggtcacacca acgaagtcaa cagcgaagtt
     6601 cacgatgcgt tattgcaagt ggttgatatg cgtcgcgaat tgctggatca actcaacaaa
     6661 cagttgggta accagctgat gatggccatt aacctgcaaa tcaaccagca gcagttaatg
     6721 agtgtgtcga aaaacctgaa atccatcctg actcagcaaa tcttttgggt gaacagtaac
     6781 cgtccaatgg actgggactg gatcaaagcg ttcccgcaaa gcctgaaaga tgaatttaag
     6841 tcgatgaaaa tcacggtgaa ctggcaaaaa gcctggcccg ccgtttttat cgctttcctc
     6901 gctggtttgc cgctgctgtt gattgccggg ctgatccact ggcgtctggg ctggctgaaa
     6961 gcgtatcaac aaaaactggc ttccgctgtg ggttccctgc gtaacgacag ccagctcaac
     7021 acaccaaaag cgatccttat cgacctgatc cgtgcgctgc cggtgtgcct gattattctc
     7081 gcggttggcc tgattctgtt gaccatgcag ctcaacatca gcgaactgct atggtcgttc
     7141 agcaaaaaac tggcgatatt ctggctggtg tttggcctgt gctggaaggt actggagaaa
     7201 aacggcgttg ccgtacgtca cttcggcatg ccggaacagc agaccagcca ctggcgtcgg
     7261 caaattgtcc gcatcagtct cgcattgctg cctatccatt tctggtctgt ggtggcagaa
     7321 ctttccccgc tgcatctgat ggatgatgtg ctggggcaag cgatgatttt cttcaacctg
     7381 ctgctgattg ccttcctggt atggccgatg tgccgcgaaa gctggcgtga taaagagtcg
     7441 cacaccatgc gactggtcac cattaccgtg ctgtcgataa tcccgattgc gctgatggtg
     7501 ctgactgcta caggctactt ctacactacg ctgcgtctgg caggacgctg gattgaaacc
     7561 gtttatctgg tgatcatctg gaacctgctg taccagacgg tactgcgtgg cttaagcgta
     7621 gcggcgcggc gtatcgcctg gcgtcgtgcg ctggcgcgtc ggcagaatct ggtgaaagag
     7681 ggcgcagaag gtgctgaacc gccggaagaa cccaccattg cactggagca agttaaccag
     7741 cagacgctgc gtattaccat gttgctgatg tttgcgctgt tcggtgtcat gttctgggca
     7801 atttggtccg atttgatcac cgtgttcagc tatctcgaca gcatcacgct ctggcattac
     7861 aacggcactg aagctggcgc tgcggtggtg aaaaacgtca ccatgggcag tctgttgttt
     7921 gcgattatcg cctcaatggt ggcctgggcg ttgattcgca acctgcctgg tttactggaa
     7981 gtgctggtgc tctcgcgact gaatatgcgc cagggcgcgt cgtatgccat tactaccatc
     8041 cttaactaca tcattattgc tgttggtgcg atgacggtgt tcggatcgct gggagtctct
     8101 tgggataaac tccagtggct ggccgcagca ttatccgtag gtcttggttt tggtttacaa
     8161 gaaattttcg gtaacttcgt ctccggtttg atcattctat tcgaacgtcc ggtgcgtatt
     8221 ggcgatacgg taaccattgg tagcttctcg gggacggtaa gtaagatccg tattcgtgcg
     8281 acaacgatta ccgatttcga tcgcaaagaa gtgatcatcc cgaacaaagc gtttgttacc
     8341 gagcgtctga tcaactggtc gttgactgac actactacgc gtctggtgat ccgtctcggc
     8401 gtagcctatg gctccgatct ggaaaaagtg cgtaaagtgt tactgaaggc ggcgactgag
     8461 cacccaagag tgatgcacga accaatgccg gaagtcttct ttacggcgtt tggtgccagc
     8521 acgttggatc atgagctgcg tctgtatgtg cgtgaactgc gtgaccgtag tcgtactgtc
     8581 gatgagctga accgtactat cgatcagctg tgccgtgaaa acgacatcaa cattgccttt
     8641 aaccagcttg tagtgcatct gcacaacgag aagggcgatg aggtgacgga agtaaaacgc
     8701 gactacaaag gcgacgatcc gacaccagcg gttgggtaat aaccaaaggg ggcgacattt
     8761 agttgccccg agattactga caaagtgcgc gttgttcatg ccggatgcgg cgtgaccgcc
     8821 ttatccggcc tacgaaaccg caagaattca atatattgca ggagcggtgt aggcctgata
     8881 agcgtagcgc atcaggcagt tttgcgtttg cccacaatct taggggacat ttagcggccc
     8941 catttatttc tcacttttcc gcctcatcat cgcgcgttaa tttcttttca taatcgcgtt
     9001 ttacaatatc cagcgcgcgc agcacggtgc gggctgggat ctgattttct tccagcagca
     9061 caatcaaatc gacagccagt ttgacatcgt caggggcatt ttcaagtgac atactctctc
     9121 cattgctagc gggttaagcg cgctagcctg ttttcgattt tttccagcgc atggcggcag
     9181 cgtgccaggc gcgcttcata ggcttccact tcacgatgca gcgtttgctg ttccacgaga
     9241 tcggtcaccc gcaccaggcg ggctctgcgt tcggcaacca tctcacgtag ccgccgctca
     9301 aaatcctgat gctgaatacg tttacgctgc cagcgggcaa ttttcggtgg cgcactgtcc
     9361 cactcgcgca atgaccaggc ggaggcttca cgcgcgatgg cttccagttg tgccgccaga
     9421 tgttccgcca gccaggccac ttgcggcagc tgttgctgct caactgcatg acgaagcgca
     9481 gccagattat cgcccgcctc gtcgagacaa gcctgtagtg ttgtcgcacg agtctgaaaa
     9541 agatgcctgt cgaaacgagc acttagcgtg gcgaactgtg acaccggggc acaacgctga
     9601 cgcagcgtag cgagctgtcc ttccagtttt tccagcagca gggcggtttt cacgataaga
     9661 cctcaatgaa aatgataatt gttatgctaa agtagccact ctgtaagctg acaacactca
     9721 atatgcaacg aatcatttta atcatcattg gctggctggc ggtagtgctg ggtacgctgg
     9781 gcgtggtatt accggtatta ccgacgacgc cgtttatcct gctggcggcc tggtgctttg
     9841 cccgttcttc cccgcgcttt cacgcctggt tgctgtaccg ctcatggttt ggcagctatc
     9901 tacgtttctg gcagaaacat catgcgatgc cgcgcggcgt caaaccgcgg gcgattttgc
     9961 ttattttgct cacgtttgcc atttctctgt ggttcgtcca gatgccatgg gtgcgcatca
    10021 tgttgctggt aattctcgcc tgtttgcttt tctatatgtg gcgaattccg gtgattgatg
    10081 aaaagcaaga aaagcactga agcacaacaa tcgcagttgc aattattgcg tacagccagt
    10141 acattctggc gttttcgagc acaggcgcag gcggtcaaag gttaaacaac tgttactttt
    10201 gatacgttta aaacgcgccg tgagtaccac cgtaacaagc aggcatacac ttatgaccgc
    10261 gactgcacag cagcttgagt atctcaaaaa tagcatcaaa agcattcagg actacccaaa
    10321 acccggcatt cttttccgcg atgtcaccag cttactggaa gacccgaaag cttacgctct
    10381 cagcatcgac ttgctggttg agcgttacaa aaatgcgggc attaccaaag ttgtcggcac
    10441 cgaagcgcgt ggcttcttgt ttggcgctcc ggtagctctg ggtctgggcg ttggctttgt
    10501 gccggtccgt aaaccgggca aactgccgcg tgaaactatc agtgaaactt acgatctgga
    10561 atacggcacc gatcagctgg agatccacgt tgatgccatc aaaccgggcg acaaagttct
    10621 ggtggtggac gatctgctgg caaccggcgg cactatcgaa gcgaccgtta aactgatccg
    10681 tcgtctgggt ggtgaagtgg ctgacgctgc gttcattatc aacctgttcg atctcggcgg
    10741 cgaacagcgt ctcgaaaaac agggcattac cagctacagc cttgtcccgt tcccgggcca
    10801 ttaattatcg ccagtcttgt gctgcccacg ctacggacag cacaagatgt gcattcagcc
    10861 tcgccgttct gacggggctg tgttagcatt accccttcgt gaatccacct tccagcgttt
    10921 cagagcctgc caatgagtta tcaggtctta gcccgaaaat ggcgcccaca aacctttgct
    10981 gacgtcgtcg gccaggaaca tgtgctgacc gcactggcga acggcttgtc gttagggcgt
    11041 attcatcatg cttatctttt ttccggcacc cgtggcgtcg gaaaaacctc tatcgcccga
    11101 ctgctggcga aggggctaaa ctgcgaaacc ggtattaccg cgacgccgtg cggcgtgtgc
    11161 gataactgtc gtgaaatcga gcaggggcgc tttgtcgatc tgattgaaat cgacgccgcc
    11221 tcgcgcacca aagttgaaga tacccgcgac ctgctggata acgtccagta cgctccggcg
    11281 cgtggtcgtt tcaaagttta tctgatcgac gaagtgcata tgctgtcgcg ccacagcttt
    11341 aacgcactgt taaaaaccct tgaagagccg ccggagcacg ttaagtttct gctggcgacg
    11401 accgatccac agaaattgcc ggtgacgatt ttgtcacgct gtctgcaatt tcatctcaag
    11461 gcgctggatg tcgagcaaat tcgccatcag cttgagcaca tcctcaacga agaacatatc
    11521 gctcacgagc cgcgggcgtt gcaattgctg gcgcgcgccg ctgaaggcag cctgcgagat
    11581 gccttaagtc tgaccgacca ggcgattgcc agcggtgacg gccaggtttc aacccaggcg
    11641 gtcagtgcga tgctgggtac gcttgacgac gatcaggcgc tgtcgctggt tgaagcgatg
    11701 gtcgaggcca acggcgagcg cgtaatggcg ctgattaatg aagccgctgc ccgtggtatc
    11761 gagtgggaag cgttgctggt ggaaatgctc ggtctgttgc atcgtattgc gatggtacaa
    11821 ctttcgcctg ctgcacttgg caacgacatg gccgccatcg agctgcggat gcgtgaactg
    11881 gcgcgcacca taccgccgac ggatattcag ctttactatc agacgctgtt gattggtcgc
    11941 aaagaattac cgtatgcgcc ggaccgccgc atgggcgttg agatgacgct gctgcgcgcg
    12001 ctggcattcc atccgcgtat gccgctgcct gagccagaag tgccacgcca gtcctttgca
    12061 cctgtcgcac caacggcagt aatgacgcca acccaggtgc cgccgcaacc gcaatcagcg
    12121 ccgcagcagg ctccgactgt accgctcccg gaaaccacca gccaggtgct ggcggcgcgc
    12181 cagcagttgc agcgcgtgca gggagcaacc aaagcaaaaa agagtgaacc ggcagccgct
    12241 acccgcgcgc ggccggtgaa taacgctgcg ctggaaagac tggcttcggt caccgatcgc
    12301 gttcaggcgc gtccggtgcc atcggcgctg gaaaaagcgc cagctaaaaa agaagcgtat
    12361 cgctggaagg cgaccactcc ggtgatgcag caaaaagaag tggtcgccac gccgaaggcg
    12421 ctgaaaaaag cgctggaaca tgaaaaaacg ccggaactgg cggcgaagct ggcggcagaa
    12481 gccattgagc gcgacgcgtg ggcggcacag gttagccaac tttcgctacc aaaactggtc
    12541 gaacaggtgg ccttaaatgc ctggaaagag gagagcgaca acgcagtatg tctgcatttg
    12601 cgcacctctc agcggcattt gaacaaccgc ggtgcacagc aaaaactggc tgaagcgttg
    12661 agcacgttaa aaggttcaac ggttgaactg actatcgttg aagatgataa tcccgcggtg
    12721 cgtacgccgc tggaatggcg tcaggcgata tacgaagaaa aacttgcgca ggcgcgcgag
    12781 tccattattg cggataataa tattcagacc ctgcgtcgat tcttcgatgc ggagctggat
    12841 gaagacagta tccgccccat ttgatcgtaa gcacagctta cgttcgtcat ccttaacgtg
    12901 attgagagag aaacctatgt ttggtaaagg cggtctgggt aacctgatga agcaagccca
    12961 gcagatgcaa gaaaaaatgc agaaaatgca ggaagagatc gcgcagctgg aagttaccgg
    13021 cgaatctggc gcaggtctgg taaaagtgac catcaacggt gcacacaact gccgtcgcgt
    13081 agagatcgac ccgagcctgc tggaagacga caaagagatg ctggaagacc tggtggctgc
    13141 agcattcaac gacgcagcac gtcgtattga agaaacgcag aaagaaaaaa tggcctctgt
    13201 atcctccgga atgcagctgc cgcctggctt taagatgccg ttctgatgca aaccagcccg
    13261 ctgttaacac agcttatgga agcactgcgc tgtctgccgg gcgttggccc gaagtcggcg
    13321 cagcgtatgg cgttcacgct gcttcagcgc gatcgtagcg gcgggatgcg tctggcgcag
    13381 gcgctcaccc gggcgatgtc ggaaatcggc cactgcgccg attgccgcac tttcaccgaa
    13441 caggaagtct gtaacatctg ttcgaatccg cgtcgtcagg aaaacggtca aatctgcgtg
    13501 gtggagagtc cggcggacat ctacgccatt gagcagacgg ggcagttttc aggtcgttat
    13561 tttgtgttga tggggcatct gtcaccgctg gacggcatcg gtccggatga tatcgggctt
    13621 gatcgtctgg aacagcgtct ggcagaggaa aaaatcactg aagtgatcct cgccaccaac
    13681 cccacggttg aaggtgaagc taccgctaac tacattgccg agctttgcgc gcaatatgac
    13741 gtggaagcca gccgaatcgc tcatggcgtt ccggttggcg gcgagctgga aatggtcgac
    13801 ggcaccacgt tgtcacactc ccttgccggg cgtcataaga ttcgttttta agcaaacgag
    13861 agcaggatca cctgctctcg cttgaaatta ttctcccttg tccccatctc tcccacatcc
    13921 tgtttttaac cttaaaatgg cattattgag gtagacctac atgaaaggac aagaaactcg
    13981 tggttttcag tcagaagtga aacagcttct gcacctgatg atccattctc tctattccaa
    14041 taaagaaatc ttcctgcgtg agcttatctc taacgcctcc gatgcggcgg acaagctgcg
    14101 tttccgtgcg ctctctaacc cggacctgta cgaaggtgat ggcgaactac gcgttcgtgt
    14161 ctctttcgat aaagacaagc gtacgctgac catctccgat aacggcgtgg ggatgacccg
    14221 cgacgaagtg attgaccatc tggggactat cgctaaatcc ggtaccaaat cattcctcga
    14281 atccctgggt tctgaccagg cgaaagacag ccagctgatc ggtcagtttg gtgttggttt
    14341 ctactctgcg tttatcgtgg ccgacaaagt gaccgtgcgt actcgcgcgg caggcgaaaa
    14401 accagaaaac ggcgtcttct gggaatcggc tggcgaaggt gaatataccg ttgccgacat
    14461 caccaaagaa gatcgtggta ctgaaatcac cctgcatctg cgtgaaggcg aagacgagtt
    14521 cctcgatgac tggcgcgtgc gttccatcat cagcaaatac tccgaccata tcgcgctgcc
    14581 ggtagagatc gaaaaacgcg aagagaaaga cggcgaaacc gttatctcct gggagaaaat
    14641 caacaaagcg caggcgctgt ggactcgtaa caagtcggaa atcaccgatg aagagtacaa
    14701 agagttctac aaacacatcg cccacgactt taatgatccg ctgacctgga gccacaaccg
    14761 tgttgaaggt aagcaggagt acaccagcct gctgtacatc ccgtcccagg ctccgtggga
    14821 tatgtggaac cgcgatcata aacacggcct gaaactgtat gttcagcgtg tgttcatcat
    14881 ggacgacgca gaacagttca tgccgaacta tctgcgcttc gtgcgtggtc tgattgactc
    14941 cagcgatctg ccgctgaacg tttcccgtga aatcctccag gacagcacgg taacgcgtaa
    15001 cctgcgcaat gcgctgacca agcgtgtgct gcaaatgctg gaaaaactgg cgaaagacga
    15061 cgcggaaaaa taccagacct tctggcaaca gtttggcctg gtactgaaag aaggtccggc
    15121 ggaagatttc gctaaccagg aagcgatcgc caaactgctg cgttttgctt ctacccatac
    15181 cgattcttct gcgcagaccg tatctctgga agactacgtt tcccgcatga aagaagggca
    15241 ggagaaaatc tactacatca ccgcagacag ctatgcggca gcgaagagca gcccgcacct
    15301 ggaactgctg cgtaagaaag gcatcgaagt tctgctgctt tccgaccgca tcgatgagtg
    15361 gatgatgaac tatctgactg agttcgacgg taaaccgttc cagtcggtgt ctaaagttga
    15421 cgagtcgctt gaaaaactgg ctgacgaagt tgatgagagc gcgaaagaag cggagaaagc
    15481 actgactccg ttcatcgacc gtgtgaaagc cctgctcggc gagcgcgtga aagatgtccg
    15541 tctgactcac cgtctgaccg atacgccagc gatcgtttcg accgacgcgg acgaaatgag
    15601 cactcagatg gcgaaactgt tcgctgcggc gggccagaaa gtgccagaag tgaaatacat
    15661 cttcgaactg aacccggatc acgtactggt gaaacgtgcg gcagatactg aagatgaagc
    15721 gaagttcagc gagtgggtag aactgctgct ggatcaggcg ctgctggcag aacgcggcac
    15781 gctggaagat ccgaacctgt ttattcgtcg tatgaaccag ctgctggttt cctgatgtca
    15841 tgccggatga cctttgtgtc atccggcatt tttcttttca tcatctgcac tttccgcaaa
    15901 ttatctcgcc attaaccgtt tcagcctcag gtgcctttct tgaggcaatc gcctgttggt
    15961 ggtatcgttt atcgcttttt caaaaaattc gacacatttt aaggggattt tcgcaatgcg
    16021 tatcattctg cttggcgctc cgggcgcggg gaaagggact caggctcagt tcatcatgga
    16081 gaaatatggt attccgcaaa tctccactgg cgatatgctg cgtgctgcgg tcaaatctgg
    16141 ctccgagctg ggtaaacaag caaaagacat tatggatgct ggcaaactgg tcaccgacga
    16201 actggtgatc gcgctggtta aagagcgcat tgctcaggaa gactgccgta atggtttcct
    16261 gttggacggc ttcccgcgta ccattccgca ggcagacgcg atgaaagaag cgggcatcaa
    16321 tgttgattac gttctggaat ttgatgtacc ggacgaactg atcgttgacc gtatcgtggg
    16381 tcgccgtgtt cacgcgccgt ctggccgtgt ttatcacgtt aaattcaatc cgccgaaagt
    16441 agaaggtaaa gacgacgtta ccggtgaaga gctgaccacc cgtaaagatg atcaggaaga
    16501 aaccgtacgt aaacgtctgg ttgaatatca tcagatgact gcgccgctga ttggctacta
    16561 ttcaaaagaa gctgaagcgg gcaacaccaa atacgcgaaa gtcgacggca ccaaaccggt
    16621 tgctgaagtc cgtgcggatc tggaaaaaat tctcggctaa tcaagccgca gcagatctgc
    16681 tttcgggcag gtctgctcat cctcctcagg caatacctct cacagcaatt agttcttctt
    16741 cctcactttt ccgctacaat tatcaacaag ttgaatcgat aagaggcggt aatgcgtcag
    16801 actaaaaccg gtatcctgct ggcaaacctg ggtacgcccg atgcccccac acctgaagcg
    16861 gtaaaacgct atctgaaaca atttttaagc gacagacgcg tggttgatac ctcacggttg
    16921 ttatggtggc cgttgctgcg cggcgtgatt ttgccgctgc gctcgccgcg tgtggcgaag
    16981 ctgtacgcct cggtctggat ggaagacggc tcgccgctga tggtttacag ccgccagcaa
    17041 cagcaggcgt tggcacaacg tttaccggat acgccagtgg cgctgggcat gagctacggc
    17101 tcgccatcac tggaaagcgc cgtcgatgaa ctcctggcag agcatgtaga tcatattgtg
    17161 gtgctgccgc tttatccgca attctcctgt tctacggtcg gtgcggtatg ggatgaactg
    17221 gcacgcattc tggcgcgcaa acgtagcatt ccggggatat cgtttattcg tgattacgcc
    17281 gataaccacg attacattaa tgcactggcg aacagcgtac gcgcttcttt tgccaaacat
    17341 ggcgaaccgg atctgctgct gctctcttat catggcattc cccagcgtta tgcagatgaa
    17401 ggcgatgatt acccgcaacg ttgccgcaca acgactcgcg aactggcttc cgcactgggg
    17461 atggcaccgg aaaaagtgat gatgaccttt cagtcgcgct ttggccggga accctggctg
    17521 atgccttata ccgacgaaac gctgaaaatg ctcggagaaa aaggcgtagg tcatatacag
    17581 gtgatgtgcc cgggctttgc tgcggattgt ctggagacgc tggaagagat tgccgagcaa
    17641 aaccgtgagg tcttcctcgg tgccggcggg aaaaaatatg aatatattcc agcgcttaat
    17701 gccacgccgg aacatattga aatgatggct aatcttgttg ccgcgtatcg ctaaagctga
    17761 gcggtaaaga actgagcgcc gtcgcgaaga gcctcgtcgg cggttttcat catccgtgaa
    17821 taatgcaaaa aggcgtgcag cgtgcctggg tagagtttga actcacaggg ctgctgatgc
    17881 gccgctaacg tctggtaaag cagacggctg tcatccagca gcggatcgaa ctccgccccg
    17941 gcaataaaac agggcggaac ttcgcgagtg agatcattat taaacaggca gtaatacggc
    18001 gactcgcggt ccgcgtcgtt gcttaaatac gcctcttcat acatctgtaa atcctgttgc
    18061 gttaagccat cccagacacc gcccaacaga cgacgagtca cggaatcccg taatccgtaa
    18121 agcccatacc acagcaaaac gcccgcaact ttaccgcaat cgatctgttt atcacgcaac
    18181 cacaacgcac tggcgagcgc cagcatggca cctgcggaat caccggcaaa gccaatgcgg
    18241 gacatattga tttgataatc ctccgcctgc tggtggaaat aacaacaagc agccacaatt
    18301 tcctctatcg cttgcggaaa acgcgcttca ggtgaaaggg tgtaatcaat accaatcacc
    18361 gtacattggc tgtagcttgc cagcaggcgc atgatgcgat cgtgggtatc gagattgccg
    18421 agaataaaac cgcctccatg caaataaaat agcgtcgctg ggctatctgg ctgcggacaa
    18481 aagagacgtg tttccacctg cccatatttt gttggaacca tgtaagctct ggttgccatt
    18541 tctggagcgc ccgcattcca gaatcggcgc tcaagcgtgt aatactgtcg ttgctcagca
    18601 atcgttcccg ttgcgggcca gggcggtaaa tccggctgaa gagtattcac aacggtcttc
    18661 atttcagcag aaataaggtc cagaacaggt agtttgtttt ccggcttcat acaaaaactc
    18721 ctttcaaatt acgtcattgt aaggaaccac tgccatgaaa atgcgatccc gcctgctgat
    18781 attgaaacta gctgcgtctc gcgcgctccc gtcagattgt gttaacattc gccgctcagt
    18841 taaccacccg taaaaacaac catgaaattt cccggtaaac gtaaatccaa acattacttc
    18901 cccgtaaacg cacgcgatcc gctgcttcag caattccagc cagaaaacga aaccagcgct
    18961 gcctgggtag tgggtatcga tcaaacgctg gtcgatattg aagcgaaagt ggatgatgaa
    19021 tttattgagc gttatggatt aagcgccggg cattcactgg tgattgagga tgatgtagcc
    19081 gaagcgcttt atcaggaact aaaacagaaa aacctgatta cccatcagtt tgcgggtggc
    19141 accattggta acaccatgca caactactcg gtgctcgcgg acgaccgttc ggtgctgctg
    19201 ggcgtcatgt gcagcaatat tgaaattggc agttatgcct atcgttacct gtgtaacact
    19261 tccagccgta ccgatcttaa ctatctacaa ggcgtggatg gcccgattgg tcgttgcttt
    19321 acgctgattg gcgagtccgg ggaacgtacc tttgctatca gtccaggcca catgaaccag
    19381 ctgcgggctg aaagcattcc ggaagatgtg attgccggag cctcggcact ggttctcacc
    19441 tcatatctgg tgcgttgcaa gccgggtgaa cccatgccgg aagcaaccat gaaagccatt
    19501 gagtacgcga agaaatataa cgtaccggtg gtgctgacgc tgggcaccaa gtttgtcatt
    19561 gccgagaatc cgcagtggtg gcagcaattc ctcaaagatc acgtctctat ccttgcgatg
    19621 aacgaagatg aagccgaagc gttgaccgga gaaagcgatc cgttgttggc atctgacaag
    19681 gcgctggact gggtagatct ggtgctgtgc accgccgggc caatcggctt gtatatggcg
    19741 ggctttaccg aagacgaagc gaaacgtaaa acccagcatc cgctgctgcc gggcgctata
    19801 gcggaattca accagtatga gtttagccgc gccatgcgcc acaaggattg ccagaatccg
    19861 ctgcgtgtat attcgcacat tgcgccgtac atgggcgggc cggaaaaaat catgaacact
    19921 aatggagcgg gggatggcgc attggcagcg ttgctgcatg acattaccgc caacagctac
    19981 catcgtagca acgtaccaaa ctccagcaaa cataaattca cctggttaac ttattcatcg
    20041 ttagcgcagg tgtgtaaata tgctaaccgt gtgagctatc aggtactgaa ccagcattca
    20101 cctcgtttaa cgcgcggctt gccggagcgt gaagacagcc tggaagagtc ttactgggat
    20161 cgttaagtta tcgtcggttc gtaggccaga taaggcgttc acgccgcatc tggcatttgg
    20221 ctctcgatgc ctgatgcgac gctggcgcgt cttatcatgc ctacatattt ttcatatttt
    20281 acatccggca accaccgttt accccgtcac cacctcaccc gccggtggcg tttccagcag
    20341 ttccagcatg gtacgggcga tttcacgctc gcccatcact acctgattcg caccacgttc
    20401 ggtgatatac gccacttcat cgtcataatg ggcgcgggca ataatctcaa tatccggatt
    20461 tttcgcgcgg gcagatgcca caatctcacc cgcttcataa ccgttgggaa tcgtcaggat
    20521 cagccatttt gcacattcca gatgcgccag ttgcataatt tcttcgttcg ccgcattgcc
    20581 caatactgcg cggaccccgc gctctcgcag ctcatcaaca cgggttcgtg acgtctcaat
    20641 caccaccagc ggaatatcag aggcgagcaa tttctccccc agcaggctgc ctacacgacc
    20701 gtaacccacc agtagcgcat ggttgcaaat atccactggg atctgcttct cttcttcgat
    20761 tgcctcttcc agcgtctgct cttccagcgt ttcggtcttc gccagatatt tctccagtag
    20821 tgcgaacagt accgggttga gcataatcga caggatcgcc cctgccagta ccaggttttg
    20881 tccggcctgc ggcagtaaat tcaatgccat tcccagtccc gccaggataa acgcgaactc
    20941 accaatctgc gccaggctgg cggcgatggt taatgccgta cgttgggagt gaccaaacag
    21001 tcgcaccagg aaaaatgcgg ctaacgactt accaaacaga ataatcgcca gcgtcgccag
    21061 cactgccagc ggttgctgaa tcagaattaa cggatcaaac aacatcccga cggagacaaa
    21121 aaacagcacc gcaaacgcgt cgcgcaatgg cagcgtatcg tgggcggcac ggtgactcag
    21181 ttcagactcg ttcagtacca tcccggcaaa gaacgcaccg agtgcaaagg agacatcaaa
    21241 cagctctacc gcaccaaagg caatccctaa cgccagcgcc agcaccgaca gggtaaacag
    21301 ctcgcgagaa ccggttgccg cgctgcgtgc cataatccac ggcaccagac ggcgacctac
    21361 cagcatcata atggcgataa atgcgatcac tttgccgatg gtgatcccca tatcgactgc
    21421 aagagtggca aagcccacat cgccctgttc catcattcct gccactgcgg gcagcaacac
    21481 cagcgtcaga accattacca ggtcttccac aatcaaccaa ccgatggcga tttgcccacg
    21541 ctgactgtca attaattgcc gttcttcaag tgcgcgcagt aacaccacgg tactggcggt
    21601 ggaaagacat aaaccgaaca cgataccggt cattaacgac cagcccagca cggcagagag
    21661 cgccataccc agcagcgtcg ccacggctat ctgggcgatc gcaccgggaa tggcgatggc
    21721 ctttaccgcc atcaaatcct tcagcgaaaa gtgcaaaccg acgccaaaca tcaacagaat
    21781 gacgcccagt tcagccagtt ccggcgcaag cttggtatcg gcaacaaagc ccggagtgaa
    21841 tggtcctgcc agcacacccg ctaacagata tcccaccaga ggagaaatac gtagtttatt
    21901 ggccagcatg ccgaggataa aggcgagcac aaggccgcca acaatggtgg tgataagcgg
    21961 ggtggcgtga tgcattccgt ctccttttcc tggtggttat tgtccatttt tggccgggaa
    22021 aaccaaaatt acaggtaata gtttatgaca atttcattga tgatgttcat gaataattat
    22081 tgaattttgc agaaaaatgg aattagctgc aaaaaaagca cggatcggaa aacagaaggc
    22141 ggtttaacag aggaaaggct tatggcgact gtaaaacgat gcagccaaag tttgcctttg
    22201 gctgcaatga aatcagtctt tatgccggtt atcaggcagg aatatggtca acatccccaa
    22261 tagtggcagg aaagcacaga ttttatagac taactcgatg ctggtgtgat cggcgataag
    22321 ccccagaact gccgctccca gacctcccat gccaaaagca aaaccgaaaa agagtccaga
    22381 aaccataccg atacgtcccg gaagtagctc ctgagcgtag accagaatgg cagagaatgc
    22441 cgaagcgagg ataaatccaa taatcaccgt taaaaccccc gtccagtgca ggctggcgta
    22501 gggtaaaatc agcgtaaacg gcgcaacgcc gaggatagag ccccaaatca catatttccg
    22561 tccaatttta tcccctacag gcccgccgat caccgtacct gccgcaacgg caaacaggaa
    22621 ggcaaacaga tgaagctggg cattctggat agataatccg aatttttgca tcagataaaa
    22681 ggtgtaatag ctgctgatgc tcgccatata gaaatatttc gagaaaatga ggattaacag
    22741 aatgctgact gccagtacca ctttgttgcg cggcagtgga ttgattatcg tcgctttggg
    22801 ttttccttta ttcattcggt gctgtgccga gtaccaacgg ctgatttgtg ccaacaccac
    22861 gatcgccagc agtgccgcaa gcacaaacca ggcaacgttg cctttgccat aaggcgcgat
    22921 aatcaccgcc gccagcaagg gtcccaggga actgccaaag ttgccgccga cctgaaagat
    22981 agattgtgcc aggccatgcc gcccgccgga agccatacgg gccacgcgag aagattccgg
    23041 atgaaagacc gatgaaccgg taccgaccag cgccgccgcc agcagaactg cgccaaaact
    23101 gcccgccagc gcaagcagca ccagaccact taaggtaaag cacatgccaa ttggcaacga
    23161 ccacggcatc ggatatttat cggtccagta gccgaccact ggttgcagta gcgaagaggc
    23221 gagctggaag gtgagggtta tcatgccaat ctgcataaat gtcagagaaa attctgactg
    23281 aagcagcgga taaatcgcca gaatcagcga ttggatcatg tcgttcagca gatgtgagag
    23341 gctgatagca cctaaaatac caaacgatgt tcgggccttg gtcgttgacg cagccgcgcc
    23401 cgccacaggc tggggttgtt cactcattgc cataggaaag tcactttttc agggttgcga
    23461 tgtaaagaat gatcttattt gtgattatta cccgactaac atacctgtat gcgtcgtctg
    23521 aaggaagtct caacgccgaa tacagaattt ctaatctgga tgcagattta tcttcaccgg
    23581 acgcagactt gtctatgatg tcgcgtcata ctatttttca acacgttgaa atcaggtcag
    23641 ggagagaagt atgaaattat tgcagcgggg cgtggcgtta gcgctgttaa ccacatttac
    23701 actggcgagt gaaactgctc tggcgtatga gcaggataaa acctacaaaa ttacagttct
    23761 gcataccaat gatcatcatg ggcatttttg gcgcaatgaa tatggcgaat atggtctggc
    23821 ggcgcaaaaa acgctggtgg atggtatccg caaagaggtt gcggctgaag gcggtagcgt
    23881 gctgctactt tccggtggcg acattaacac tggcgtgccc gagtctgact tacaggatgc
    23941 cgaacctgat tttcgcggta tgaatctggt gggctatgac gcgatggcga tcggtaatca
    24001 tgaatttgat aatccgctca ccgtattacg ccagcaggaa aagtgggcca agttcccgtt
    24061 gctttccgcg aatatctacc agaaaagtac tggcgagcgc ctgtttaaac cgtgggcgct
    24121 gtttaagcgt caggatctga aaattgccgt tattgggctg acaaccgatg acacagcaaa
    24181 aattggtaac ccggaatact tcactgatat cgaatttcgt aagcccgccg atgaagcgaa
    24241 gctggtgatt caggagctgc aacagacaga aaagccagac attattatcg cggcgaccca
    24301 tatggggcat tacgataatg gtgagcacgg ctctaacgca ccgggcgatg tggagatggc
    24361 acgcgcgctg cctgccggat cgctggcgat gatcgtcggt ggtcactcgc aagatccggt
    24421 ctgcatggcg gcagaaaaca aaaaacaggt cgattacgtg ccgggtacgc catgcaaacc
    24481 agatcaacaa aacggcatct ggattgtgca ggcgcatgag tggggcaaat acgtgggacg
    24541 ggctgatttt gagtttcgta atggcgaaat gaaaatggtt aactaccagc tgattccggt
    24601 gaacctgaag aagaaagtga cctgggaaga cgggaaaagc gagcgcgtgc tttacactcc
    24661 tgaaatcgct gaaaaccagc aaatgatctc gctgttatca ccgttccaga acaaaggcaa
    24721 agcgcagctg gaagtgaaaa taggcgaaac caatggtcgt ctggaaggcg atcgtgacaa
    24781 agtgcgtttt gtacagacca atatggggcg gttgattctg gcagcccaaa tggatcgcac
    24841 tggtgccgac tttgcggtga tgagcggagg cggaattcgt gattctatcg aagcaggcga
    24901 tatcagctat aaaaacgtgc tgaaagtgca gccattcggc aatgtggtgg tgtatgccga
    24961 catgaccggt aaagaggtga ttgattacct gaccgccgtc gcgcagatga agccagattc
    25021 aggtgcctac ccgcaatttg ccaacgttag ctttgtggcg aaagacggca aactgaacga
    25081 ccttaaaatc aaaggcgaac cggtcgatcc ggcgaaaact taccgtatgg cgacattaaa
    25141 cttcaatgcc accggcggtg atggatatcc gcgccttgat aacaaaccgg gctatgtgaa
    25201 taccggcttt attgatgccg aagtgctgaa agcgtatatc cagaaaagct cgccgctgga
    25261 tgtgagtgtt tatgaaccga aaggtgaggt gagctggcag taatccgaaa gtgccggatg
    25321 tttgcatccg gcacaatgct taatcgcggc gggcgatatc agcaaatttg gcatcgagga
    25381 tctttgccag atcgcctgcc gccagttcga tatccagtcc gcgcttgccg ccggaaacat
    25441 aaatagtggc aaattcttgt gcgggggcgt cgataatcgt tggcagacgt tttttctgcc
    25501 ccagtgggct aatcccccca accaggtatc ccgtcgaacg ctgcgcgacc atcggatcgg
    25561 ccatctcaac tttcttggca cccagcgctt ttgctacttt tttaagatcc agttgacctg
    25621 cgaccggcgt aacggccacg gcaaggtgtt tcatatcacc gttcactgcc accagcagcg
    25681 ttttgtagac ctgatccgga ttcaaaccta attttttgac gacttcatcg ccaaaattgg
    25741 tttcagccgg atcgtgctcg taggtatgga tttgaaacga aatcttgttt ttttcgagta
    25801 atttaactgc gggtgtcatg gtaattttcc tgccttaaac aaaaaaacga agcaagcata
    25861 cgcctgatgg cggtccaaaa aaaagagtca tcttgcctaa gagtattggc aggatggtga
    25921 gattgagcga caatcgagtt acaccgtcgc ttaaagtgac ggcataataa taaaaaaatg
    25981 aaattcctct ttgacgggcc aatagcgata ttggccattt ttttagcgca acatttgcgg
    26041 caaattccct tctccataca ggtgtagtgc accgaccgcg accacatatc gccccggcgg
    26101 catggcgcgt aatttatccc gccaggcgag atttcgctga tgcatcagca catcgtacag
    26161 cgactgactg aacgtattgg gcagcgttat atcattattt tgcggaggtg cattcagcca
    26221 ccagctcatc atttgttgca gcaaccgtgc gttggtatgc cagtgggtca gcgtatcgtc
    26281 cagcagcgcc agtcctttgt cagggagctg gagcaacatg gtaatctggt tttcagcccc
    26341 ttccagttca atcacgggtt tatgttgttg cttcgccgcc tgcaataact ggtaatcgat
    26401 accgtattct gcccgcagcc ccagtttttg tgcctgcgtc gcctgaagaa ccatcgcgat
    26461 ttgccacagc ggttgagtag aaaagagcga cggagaaatg cccatctcct ggctaatgtg
    26521 ctgcagattt tggagttgct cctcgttaat gcgctcttcc agcgcctcgc aagtaggcaa
    26581 attagcaaaa ggcgtatcgc tggtggaaac atccgcctcg acgatcagcg catcggcgtt
    26641 tttgagcttt ttgagcaaac gggtgggcag gggagccata tcgtggctac ccatatgaat
    26701 actgccaatc agatgaaaat ggcgattgcc gggaagggtg atatcgatgg caggccaggt
    26761 gtaatgatta ccgcgcagcg cggcccaaag cgtttttacc cggtacaaca gatccatacg
    26821 acctcccttt gtgaaatatc atgctagcgc gcggggaggg atggcgcaaa tggagttggg
    26881 gacttgttta atgtgtttgt atgattcagt atgttcttgt atcgctattc acagggaagc
    26941 aacagttaaa aactatgaaa caggcaacaa gaaaaccgac gacacccgga gatattcttc
    27001 tctatgaata tctggaaccg ctcgatttga aaatcaatga gttagcagag ttgctgcatg
    27061 ttcatcgtaa tagcgtcagt gcactgatca ataacaatcg taaactcact actgagatgg
    27121 catttcgtct ggcgaaagtt tttgatacca cagtcgattt ttggctaaac ctccaggcgg
    27181 cggttgatct ttgggaagtt gaaaacaaca tgcgcaccca ggaagaattg ggacggattg
    27241 aaacagtggc tgaatattta gcacgccgtg aagagcgtac aaaaaaggtc gcgtaaggcc
    27301 aaaacatcgc ttcatctcgg atgccggata aggcgttcac gc
//
LOCUS       DAPHBJ010000046        26923 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.046, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000046 DAPHBJ010000000
VERSION     DAPHBJ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.046"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            16..1485
                     /gene="gspA"
                     /locus_tag="RHZ76_001719"
     CDS             16..1485
                     /gene="gspA"
                     /locus_tag="RHZ76_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HDV2812372.1"
                     /translation="MSTRREVILSWLCEKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSIGRLTEH
                     ELKASILEGQNIDQPDLLLTARVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIALTCGWLLLSSFTATLPVPAWLIPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSGNASLQTL
                     VDQNLPWIASLKVGDKKLPVVVVRVGEASVDVLVGQQTWTLTHKWFESVWTGDYLLLW
                     KMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPLLVEKIKQFQKSHHLK
                     TDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            1487..1906
                     /gene="gspB"
                     /locus_tag="RHZ76_001720"
     CDS             1487..1906
                     /gene="gspB"
                     /locus_tag="RHZ76_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HDV2812373.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWVLWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPGVA
                     VENAPSSSEDEENTVEESEEKAGLRERVKNALNELER"
     gene            2144..2455
                     /gene="rpsJ"
                     /locus_tag="RHZ76_001721"
     CDS             2144..2455
                     /gene="rpsJ"
                     /locus_tag="RHZ76_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDV2812374.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2488..3117
                     /gene="rplC"
                     /locus_tag="RHZ76_001722"
     CDS             2488..3117
                     /gene="rplC"
                     /locus_tag="RHZ76_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDV2812375.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3128..3733
                     /gene="rplD"
                     /locus_tag="RHZ76_001723"
     CDS             3128..3733
                     /gene="rplD"
                     /locus_tag="RHZ76_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDV2812376.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3730..4032
                     /gene="rplW"
                     /locus_tag="RHZ76_001724"
     CDS             3730..4032
                     /gene="rplW"
                     /locus_tag="RHZ76_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDV2812377.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRGDWKKAYVTLKEGQNLDFVGGAE"
     gene            4050..4871
                     /gene="rplB"
                     /locus_tag="RHZ76_001725"
     CDS             4050..4871
                     /gene="rplB"
                     /locus_tag="RHZ76_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDV2812378.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4888..5166
                     /gene="rpsS"
                     /locus_tag="RHZ76_001726"
     CDS             4888..5166
                     /gene="rpsS"
                     /locus_tag="RHZ76_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDV2812379.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5181..5513
                     /gene="rplV"
                     /locus_tag="RHZ76_001727"
     CDS             5181..5513
                     /gene="rplV"
                     /locus_tag="RHZ76_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDV2812380.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5531..6232
                     /gene="rpsC"
                     /locus_tag="RHZ76_001728"
     CDS             5531..6232
                     /gene="rpsC"
                     /locus_tag="RHZ76_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDV2812381.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6245..6655
                     /gene="rplP"
                     /locus_tag="RHZ76_001729"
     CDS             6245..6655
                     /gene="rplP"
                     /locus_tag="RHZ76_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDV2812382.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6655..6846
                     /gene="rpmC"
                     /locus_tag="RHZ76_001730"
     CDS             6655..6846
                     /gene="rpmC"
                     /locus_tag="RHZ76_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDV2812383.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6846..7100
                     /gene="rpsQ"
                     /locus_tag="RHZ76_001731"
     CDS             6846..7100
                     /gene="rpsQ"
                     /locus_tag="RHZ76_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDV2812384.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7265..7636
                     /gene="rplN"
                     /locus_tag="RHZ76_001732"
     CDS             7265..7636
                     /gene="rplN"
                     /locus_tag="RHZ76_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDV2812385.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7647..7961
                     /gene="rplX"
                     /locus_tag="RHZ76_001733"
     CDS             7647..7961
                     /gene="rplX"
                     /locus_tag="RHZ76_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDV2812386.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7976..8515
                     /gene="rplE"
                     /locus_tag="RHZ76_001734"
     CDS             7976..8515
                     /gene="rplE"
                     /locus_tag="RHZ76_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDV2812387.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8530..8835
                     /gene="rpsN"
                     /locus_tag="RHZ76_001735"
     CDS             8530..8835
                     /gene="rpsN"
                     /locus_tag="RHZ76_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDV2812388.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8869..9261
                     /gene="rpsH"
                     /locus_tag="RHZ76_001736"
     CDS             8869..9261
                     /gene="rpsH"
                     /locus_tag="RHZ76_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDV2812389.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9274..9807
                     /gene="rplF"
                     /locus_tag="RHZ76_001737"
     CDS             9274..9807
                     /gene="rplF"
                     /locus_tag="RHZ76_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDV2812390.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9817..10170
                     /gene="rplR"
                     /locus_tag="RHZ76_001738"
     CDS             9817..10170
                     /gene="rplR"
                     /locus_tag="RHZ76_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDV2812391.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10185..10688
                     /gene="rpsE"
                     /locus_tag="RHZ76_001739"
     CDS             10185..10688
                     /gene="rpsE"
                     /locus_tag="RHZ76_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDV2812392.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10692..10871
                     /gene="rpmD"
                     /locus_tag="RHZ76_001740"
     CDS             10692..10871
                     /gene="rpmD"
                     /locus_tag="RHZ76_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDV2812393.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10875..11309
                     /gene="rplO"
                     /locus_tag="RHZ76_001741"
     CDS             10875..11309
                     /gene="rplO"
                     /locus_tag="RHZ76_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDV2812394.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEIRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11317..12648
                     /gene="secY"
                     /locus_tag="RHZ76_001742"
     CDS             11317..12648
                     /gene="secY"
                     /locus_tag="RHZ76_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDV2812395.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12680..12796
                     /gene="rpmJ"
                     /locus_tag="RHZ76_001743"
     CDS             12680..12796
                     /gene="rpmJ"
                     /locus_tag="RHZ76_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDV2812396.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12943..13299
                     /gene="rpsM"
                     /locus_tag="RHZ76_001744"
     CDS             12943..13299
                     /gene="rpsM"
                     /locus_tag="RHZ76_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDV2812397.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13316..13705
                     /gene="rpsK"
                     /locus_tag="RHZ76_001745"
     CDS             13316..13705
                     /gene="rpsK"
                     /locus_tag="RHZ76_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDV2812398.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13739..14359
                     /gene="rpsD"
                     /locus_tag="RHZ76_001746"
     CDS             13739..14359
                     /gene="rpsD"
                     /locus_tag="RHZ76_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDV2812399.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14385..15374
                     /gene="rpoA"
                     /locus_tag="RHZ76_001747"
     CDS             14385..15374
                     /gene="rpoA"
                     /locus_tag="RHZ76_001747"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDV2812400.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15415..15798
                     /gene="rplQ"
                     /locus_tag="RHZ76_001748"
     CDS             15415..15798
                     /gene="rplQ"
                     /locus_tag="RHZ76_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDV2812401.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15905..16273
                     /locus_tag="RHZ76_001749"
     CDS             15905..16273
                     /locus_tag="RHZ76_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HDV2812402.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            16284..16709
                     /gene="zntR"
                     /locus_tag="RHZ76_001750"
     CDS             16284..16709
                     /gene="zntR"
                     /locus_tag="RHZ76_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HDV2812403.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16765..16983
                     /gene="arfA"
                     /locus_tag="RHZ76_001751"
     CDS             16765..16983
                     /gene="arfA"
                     /locus_tag="RHZ76_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HDV2812404.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16980..17390)
                     /gene="mscL"
                     /locus_tag="RHZ76_001752"
     CDS             complement(16980..17390)
                     /gene="mscL"
                     /locus_tag="RHZ76_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDV2812405.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17520..18896)
                     /gene="trkA"
                     /locus_tag="RHZ76_001753"
     CDS             complement(17520..18896)
                     /gene="trkA"
                     /locus_tag="RHZ76_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HDV2812406.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18918..20207)
                     /gene="rsmB"
                     /locus_tag="RHZ76_001754"
     CDS             complement(18918..20207)
                     /gene="rsmB"
                     /locus_tag="RHZ76_001754"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDV2812407.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKTGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20253..21200)
                     /gene="fmt"
                     /locus_tag="RHZ76_001755"
     CDS             complement(20253..21200)
                     /gene="fmt"
                     /locus_tag="RHZ76_001755"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDV2812408.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVAELQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21215..21724)
                     /gene="def"
                     /locus_tag="RHZ76_001756"
     CDS             complement(21215..21724)
                     /gene="def"
                     /locus_tag="RHZ76_001756"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDV2812409.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21854..22978
                     /gene="dprA"
                     /locus_tag="RHZ76_001757"
     CDS             21854..22978
                     /gene="dprA"
                     /locus_tag="RHZ76_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDV2812410.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWVAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22950..23423
                     /locus_tag="RHZ76_001758"
     CDS             22950..23423
                     /locus_tag="RHZ76_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HDV2812411.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23452..23994
                     /locus_tag="RHZ76_001759"
     CDS             23452..23994
                     /locus_tag="RHZ76_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HDV2812412.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCINYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23999..24571
                     /gene="tsaC"
                     /locus_tag="RHZ76_001760"
     CDS             23999..24571
                     /gene="tsaC"
                     /locus_tag="RHZ76_001760"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HDV2812413.1"
                     /translation="MNNNLQRDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24576..25394
                     /gene="aroE"
                     /locus_tag="RHZ76_001761"
     CDS             24576..25394
                     /gene="aroE"
                     /locus_tag="RHZ76_001761"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDV2812414.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLMRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALSMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25391..25648
                     /locus_tag="RHZ76_001762"
     CDS             25391..25648
                     /locus_tag="RHZ76_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HDV2812415.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25624..26178)
                     /locus_tag="RHZ76_001763"
     CDS             complement(25624..26178)
                     /locus_tag="RHZ76_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDV2812416.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            26652..>26923
                     /locus_tag="RHZ76_001764"
     rRNA            26652..>26923
                     /locus_tag="RHZ76_001764"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         6883 a         6343 c         7055 g         6642 t
ORIGIN      
        1 aaaacaggaa gttctatgtc tacgagaaga gaagttattc tttcctggtt gtgtgagaaa
       61 cgtcaaacct ggcgtctatg ctatttgttg ggtgaggctg gaagtggaaa aacctggctg
      121 gcgcagcaac tgcaaaaaga taaacatcgc cgtgtgatta ctttaagtct cgttgtttcc
      181 tggcaaggta aggccgcatg gatcgttacc gacgataacg cggctgaaca gggctgccgt
      241 gacagcgcct ggacgcgaga tgagatggcg gggcaattac tgcatgcgct tcatcgaact
      301 gatagtcgct gccctcttat tattattgaa aatgctcacc tgaatcaccg ccgcatactt
      361 gatgatctcc aacgcgcaat atctcttatt cctgacggtc aatttttgct gatcggcaga
      421 cccgatcgaa aagtcgaacg tgattttaaa aaacagggca ttgaacttgt ctcgatagga
      481 cgtctgacgg agcacgaact taaggcaagc atccttgaag gtcagaatat tgaccaaccc
      541 gatctccttt taaccgccag agttctgaaa cggatagctt tattatgtcg gggcgatcgc
      601 agaaagctgg cgcttgccgg cgaaacaatt cgcttattac agcaagccga gcagaccagc
      661 gttttcacag caaaacaatg gcgaatgatt taccgcatat tgggtgataa ccgcccccgg
      721 aaaatgcagc ttgccgtggt gatgagtgga accattatcg ctcttacctg tggttggcta
      781 ctgctgtcct cttttacagc tacgttaccc gttcctgcgt ggctgatacc cgtaacgcca
      841 gtagttaaac aagatatgac taaggatatt gctcatgtgg tgatgcgcga tagtgaggcg
      901 ctaagcgtgt tatacggcgt atgggggtac gaggtcccag ctgattcggc ctggtgcgat
      961 caggcagtaa gagcggggct ggcatgtaaa agtgggaatg cttctttaca aacattggta
     1021 gatcagaatt tgccctggat cgcatcgctt aaagtcgggg acaaaaaatt gcctgttgtg
     1081 gtcgttcgcg tcggtgaggc tagcgttgat gtcctcgtag gtcagcaaac gtggacgctg
     1141 acacataaat ggtttgagtc ggtatggacg ggtgattatc ttctgttgtg gaaaatgtcg
     1201 ccggaagggg agagtaccat tacgcgtgat agcagcgagg aagagattct ctggctggaa
     1261 acgatgttaa accgcgcatt gcatatttcg acagaacctt cggcggagtg gcgtcccttg
     1321 ttggtagaaa aaataaagca atttcaaaaa agccaccact tgaaaactga cggtgttgtt
     1381 ggcttttcaa cgttagtaca tctctggcag gtggcagggg agagtgccta cttatatcgg
     1441 gatgaagcaa atatttcccc ggaaacaaca gtgaagggga aatgacatgt ttgaattcta
     1501 tatcgccgcg cgtgagcaga aagaaacagg tcaccctggt atcttctccc gacaaaaaca
     1561 cagcaccata atatatgtaa tttgtttatt actgatttgc ctgtggtttg caggaatggt
     1621 gctggttggt gggtatgcca ggcagctatg ggtgctttgg atagtaaaag ccgaagtcac
     1681 tgtagaggct gaaacgcccg cttttaaaca atcaacacaa cactatttct tcaaaaaaca
     1741 gccactcccc gtcgtcgaat ccgtcgagga ggaagatgac ccaggcgtcg cggttgagaa
     1801 tgccccctct tcttctgaag acgaagaaaa tactgtggaa gaaagcgaag agaaagccgg
     1861 tttaagagag cgtgtcaaaa atgcactgaa cgaacttgag agataacccg aaggctgttt
     1921 actttactag gaatacgctt gcgttcggtg gttaagtatg tataatgcgc gggcttgtcg
     1981 tagttgacag caggttcaat ctgaacctca gttagccgaa tttggctacc taacaatgct
     2041 cccaatcggg gagctacgta agaacggtta cactctccca tcaatcgtaa tgggtctgag
     2101 gagtaatcat tttcgtttat aaaataattg gagctctggt ctcatgcaga accaaagaat
     2161 ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat caagcaaccg cggaaatcgt
     2221 cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg atcccgctgc cgacacgcaa
     2281 agagcgcttc actgttctga tctccccgca cgtcaacaaa gacgcgcgcg atcagtacga
     2341 aatccgtact cacttgcgtc tggttgacat cgttgagcca accgagaaaa ccgttgatgc
     2401 tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag atcagcctgg gttaatcagg
     2461 tcattgagcg attgagaggt tgaaacaatg attggtttag tcggtaaaaa agtgggtatg
     2521 acccgtatct tcacagaaga cggcgtttct atcccagtaa ccgtaatcga agttgaagca
     2581 aaccgcgtta ctcaggttaa agacctggct aacgatggct accgtgctat tcaggtgacc
     2641 accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag ctggccactt cgctaaagct
     2701 ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg ctgaaggcga agagttcact
     2761 gtaggtcaga gcattagcgt tgaactgttt gctgacgtta aaaaagttga cgtaactggc
     2821 acctctaaag gtaaaggttt cgcaggtacc gttaagcgct ggaacttccg tacccaggac
     2881 gctactcacg gtaactcctt gtctcaccgc gttccgggtt ctatcggtca gaaccagact
     2941 ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga tgggtaacga acgtgtaacc
     3001 gttcagagcc ttgacgtagt acgcgttgac gctgagcgca acctgctgct ggttaaaggt
     3061 gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac cagctgtgaa ggcgtaagga
     3121 gatagcaatg gaattagtat tgaaagacgc gcagagcgcg ctgactgttt ccgaaactac
     3181 cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt gttgttgctt atgcagctgg
     3241 tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa gtaactggtt ccggtaaaaa
     3301 accgtggcgc cagaaaggca ccggccgtgc gcgttctggt tctatcaaga gcccgatctg
     3361 gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac cacagtcaaa aagttaacaa
     3421 gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa ctggtacgtc aggatcgtct
     3481 gatcgttgtc gagaagttct ctgtagaagc gccgaaaact aagctgctgg cacagaaact
     3541 gaaagacatg gctctggaag atgtgctgat catcaccggt gagctggacg aaaacctgtt
     3601 cctggctgcg cgcaacctgc acaaggttga cgtacgcgat gcaactggta tcgacccggt
     3661 tagcctgatc gccttcgaca aagtcgtaat gactgctgat gctgttaagc aagttgagga
     3721 gatgctggca tgattcgtga agaacgtctg ctgaaggtgc tgcgtgcacc gcacgtttct
     3781 gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg tactcaaagt tgctaaagac
     3841 gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt ttgaagtcga agtcgaagtc
     3901 gttaacaccc tggtagttaa agggaaagtt aaacgtcacg gacagcgtat cggtcgtcgt
     3961 ggcgactgga aaaaagctta cgtcaccctg aaagaaggcc agaatctgga cttcgttggc
     4021 ggcgctgagt aagtcggagg agtaatacaa tggcagttgt taaatgtaaa ccgacatctc
     4081 cgggtcgtcg ccacgtagtt aaagtggtta accctgagct gcacaagggc aaaccttttg
     4141 ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa caacaatggc cgtatcacca
     4201 ctcgtcatat cggtggtggc cacaagcagg cttaccgtat tgttgacttc aaacgcaaca
     4261 aagacggtat cccggcagtt gttgaacgtc ttgagtacga tccgaaccgt tccgcgaaca
     4321 tcgcgctggt tctgtacaaa gacggtgaac gccgttacat cctggcccct aaaggcctga
     4381 aagctggcga ccagattcag tctggcgttg atgctgcaat caaaccaggt aacaccctgc
     4441 cgatgcgcaa catcccggtt ggttctactg ttcataacgt agaaatgaaa ccaggtaaag
     4501 gcggtcagct ggcacgttcc gctggtactt acgttcagat cgttgctcgt gatggtgctt
     4561 atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt agaagcagac tgccgtgcaa
     4621 ctctgggcga agttggcaat gctgagcata tgctgcgcgt tctgggtaaa gcaggtgctg
     4681 cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc gatgaacccg gtagaccacc
     4741 cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc ggtaactccg tggggcgttc
     4801 agaccaaagg taagaagacc cgcagcaaca agcgtactga taaattcatc gtacgtcgcc
     4861 gtagcaaata attttagagg ataagccatg ccacgttctc tcaagaaagg tccttttatt
     4921 gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa gcggagacaa gaagcccctg
     4981 cgcacttggt cccgtcgttc aacgatcttt cctaacatga tcggtttgac catcgctgtc
     5041 cataatggtc gtcagcacgt tccggtattt gtaaccgacg aaatggttgg tcacaaactg
     5101 ggtgaattcg caccgactcg tacttatcgc ggccacgctg ctgataaaaa agcgaagaag
     5161 aaataaggta ggaggaagag atggaaacta tcgctaaaca tcgccatgct cgttcttctg
     5221 ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa gaaagtgtcg caggctctgg
     5281 atattttgac ctacaccaac aagaaagcgg ctgtactggt caagaaagtt ctggaatctg
     5341 ccattgctaa cgctgaacac aacgatggcg ctgacattga cgatctgaaa gttacgaaaa
     5401 ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc gcgtgcaaaa ggtcgtgcag
     5461 atcgcatcct gaagcgcacc agccacatca ctgtggttgt gtccgatcgc tgagactctg
     5521 gagactagca atgggtcaga aagtacatcc taatggtatt cgcctgggta ttgtaaaacc
     5581 atggaactct acctggtttg cgaacaccaa agaattcgct gacaacctgg acagcgattt
     5641 taaagtacgt cagtacctga ctaaggaact ggctaaagcg tccgtatctc gtatcgttat
     5701 cgagcgtccg gctaagagca tccgtgtaac cattcacact gctcgcccgg gtatcgttat
     5761 cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc gtagcggaca tcgctggcgt
     5821 tcctgcacag atcaacatcg ccgaagttcg taagcctgaa ctggacgcaa aactggttgc
     5881 tgacagcatc acttctcagc tggaacgtcg cgttatgttc cgtcgtgcta tgaagcgtgc
     5941 tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa gttgaagtta gcggccgtct
     6001 gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa ggtcgcgtac cgctgcacac
     6061 tctgcgtgct gacatcgact acaacacctc tgaagcgcac accacttacg gtgtaatcgg
     6121 cgttaaagtg tggatcttca aaggcgagat cctgggtggt atggctgctg ttgaacaacc
     6181 ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa ggccgtaaat aaggagcgtc
     6241 gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg cacaaaggcc gtaaccgcgg
     6301 tctggcgcag ggtacggatg ttagcttcgg cagcttcggt ctgaaagctg ttggccgtgg
     6361 tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct atgacccgtg cagttaagcg
     6421 tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg atcactgaaa agccgctggc
     6481 agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg gttgccttga ttcagccggg
     6541 taaagtcctg tatgaaatgg acggtgttcc ggaagagctg gcccgtgaag cattcaagct
     6601 ggcagcagcg aaactgccga ttaaaaccac ctttgtaact aagacggtga tgtaatgaaa
     6661 gcaaaagagc tgcgtgagaa gagcgttgaa gagctgaaca ccgagctgct gaacctgctg
     6721 cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc agctgcaaca gtctcacctg
     6781 ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt tactgaacga gaaggcgggt
     6841 gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt tgttagcgac aaaatggaga
     6901 aatccattgt tgttgctatc gaacgttttg tgaaacaccc gatctacggt aaattcatca
     6961 agcgtacgac caaactgcac gtacatgacg agaacaacga atgcggtatc ggtgacgtgg
     7021 ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc ctggacgctg gttcgcgttg
     7081 tagagaaagc ggttctgtaa tacagtacac tctctcaata cgaataaacg gctcagaaat
     7141 gagccgttta ttttttctac ccatatcctt gaagcggtgt tataatgccg cgccctcgat
     7201 atggggattt ttaacgacct gattttcggg tctcagtagt agttgacatt agcggagcac
     7261 taaaatgatc caagaacaga ctatgctgaa cgtcgccgac aactccggtg cacgtcgcgt
     7321 aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac gcaggcgtag gcgacatcat
     7381 caagatcacc atcaaagaag caattccgcg tggtaaggtc aaaaaaggtg atgtgctgaa
     7441 ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg gacggttctg tcattcgctt
     7501 cgatggtaat gcttgtgttc ttctgaacaa caacagcgag cagcctatcg gtacgcgtat
     7561 ttttgggccg gtaactcgtg agcttcgtag tgagaagttc atgaaaatta tctctctggc
     7621 accagaagta ctctaaggag cgaatcatgg cagcgaaaat ccgtcgtgat gacgaagtta
     7681 tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt taagaatgtc ctgtcttccg
     7741 gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca tcagaagccg gttccggccc
     7801 tgaaccaacc gggtggcatc gttgaaaaag aagccgctat tcaggtttcc aacgtagcaa
     7861 tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt tagattcgaa gacggtaaaa
     7921 aagtccgttt cttcaagtct aacagcgaaa ctatcaagta atttggagta gtacgatggc
     7981 gaaactgcat gattactaca aagacgaagt agttaaaaaa ctcatgactg agtttaacta
     8041 caattctgtc atgcaagtcc ctcgggtcga gaagatcacc ctgaacatgg gtgttggtga
     8101 agcgatcgct gacaaaaaac tgctggataa cgcagcagca gacctggcag caatctccgg
     8161 tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca ggcttcaaaa tccgtcaggg
     8221 ctatccgatc ggctgtaaag taactctgcg tggcgaacgc atgtgggagt tctttgagcg
     8281 cctgatcact attgctgtac ctcgtatccg tgacttccgt ggcctgtccg ctaagtcttt
     8341 cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag atcatcttcc cagaaatcga
     8401 ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc attaccacta ctgcgaaatc
     8461 tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc ccgttccgca agtaaggtag
     8521 ggttactaaa tggctaagca atcaatgaaa gcacgcgaag taaaacgcgt agctttagct
     8581 gataaatact tcgcgaaacg cgctgaactg aaagcgatca tctctgatgt gaacgcttcc
     8641 gacgaagatc gttggaacgc tgttctcaag ctgcagactc tgccgcgtga ttccagcccg
     8701 tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgtccgc atggtttcct gcggaagttc
     8761 gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg gtgaaatccc gggtctgaaa
     8821 aaggctagct ggtaattgtc accaattgaa tcacgggagt aaagacagat gagcatgcaa
     8881 gatccgatcg cggatatgct gacccgtatc cgtaacggtc aggccgcgaa caaagctgcg
     8941 gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca acgtgctgaa ggaagaaggt
     9001 tttattgaag attttaaagt tgaaggcgac accaagcctg aactggaact tactctgaag
     9061 tatttccagg gcaaagctgt tgtagaaagc attcagcgtg tcagccgccc aggtctgcgc
     9121 atctataaac gtaaagatga gctgccgaaa gttatggcgg gtctgggtat cgcagttgtt
     9181 tctacctcta aaggtgttat gactgatcgt gcagcgcgcc aggctggtct tggtggcgaa
     9241 attatctgct acgtagccta atcggaggaa aaaatgtctc gtgttgctaa agcaccggtc
     9301 gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg ttattacgat caaaggtaaa
     9361 aacggcgagc tgactcgtac tctcaacgat gctgttgaag ttaaacatgc agataatacc
     9421 ctgaccttcg gtccgcgtga tggttacgca gacggttggg cacaggctgg taccgcgcgt
     9481 gccctgctga actcaatggt tatcggtgtt accgaaggct tcactaagaa gctgcagctg
     9541 gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga ttaacctgtc tctgggtttc
     9601 tctcatcctg ttgaccatca gctgcctgcg ggtatcactg ctgaatgtcc gactcagact
     9661 gaaatcgtgc tgaaaggcgc tgataagcag gtgatcggcc aggttgcagc ggatctgcgc
     9721 gcctaccgtc gtcctgagcc ttataaaggc aagggtgttc gttacgccga cgaagtcgtg
     9781 cgtaccaaag aggctaagaa gaagtaaggt aacactatgg ataagaaatc tgctcgtatc
     9841 cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg gcgcaactcg cctggtggta
     9901 catcgtaccc cgcgtcacat ttacgcacag gtaattgcac cgaacggttc tgaagttctg
     9961 gtagctgctt ctactgtaga aaaagctatc gctgaacaac tgaagtacac cggtaacaaa
    10021 gacgcggctg cagctgtggg taaagctgtc gctgaacgcg ctctggaaaa aggcatcaaa
    10081 gatgtatcct ttgaccgttc cgggttccaa tatcatggtc gtgtccaggc actggcagat
    10141 gctgcccgtg aagctggcct tcagttctaa ggtagaggtg taagatggct cacatcgaaa
    10201 aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa ccgcgtatct aaaaccgtta
    10261 aaggtggtcg tattttctcc ttcacagctc tgactgtagt tggcgatggt aacggtcgcg
    10321 ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc gatccagaaa gcgatggaaa
    10381 aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg cactctgcaa caccctgtta
    10441 aaggtgttca cacgggttct cgcgtattca tgcagccggc ttccgaaggt accggtatca
    10501 tcgccggtgg tgcaatgcgc gccgttctgg aagtcgctgg ggttcataac gttctggcta
    10561 aagcctatgg ttccaccaac ccgatcaacg tggttcgtgc aactattgat ggcctggaaa
    10621 atatgaattc tccagaaatg gtcgctgcca agcgtggtaa atccgttgaa gaaattctgg
    10681 ggaaataaac catggcaaag actattaaaa ttactcaaac ccgcagtgca atcggtcgtc
    10741 tgccgaaaca caaggcaacg ctgcttggcc tgggtctgcg tcgtattggt cacaccgtag
    10801 agcgcgagga tactcctgct attcgcggta tgatcaacgc ggtttccttc atggttaaag
    10861 ttgaggagta agagatgcgt ttaaatactc tgtctccggc cgaaggctcc aaaaaggcgg
    10921 gtaaacgcct gggtcgtggt atcggttctg gcctcggtaa aaccggtggt cgtggtcaca
    10981 aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg tttcgagggt ggtcagatgc
    11041 ctctgtaccg tcgtctgccg aaattcggct tcacttctcg taaagcagcg attacagccg
    11101 aaattcgtct gtctgacctg gctaaagtag aaggcggtgt agtagacctg aacacgctga
    11161 aagcggctaa cattatcggt atccagatcg agttcgcgaa agtgatcctg gctggcgaag
    11221 taacgactcc ggtaactgtt cgtggcctgc gtgttactaa aggcgctcgt gctgctatcg
    11281 aagctgctgg cggtaaaatc gaggaataag tagcagatgg ctaaacaacc gggattagat
    11341 tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca gactgctgtt tgttatcggt
    11401 gcgctgattg tgttccgtat tggctctttt attccgatcc ctggtattga tgccgctgta
    11461 cttgccaaac tgcttgagca acagcgaggc accatcattg agatgtttaa catgttctct
    11521 ggtggtgctc tcagccgtgc ttctatcttt gctctgggga tcatgccgta tatttcggcg
    11581 tcgatcatta tccagctgct gacggtggtt cacccaacgt tggcagaaat taagaaagaa
    11641 ggggagtctg gtcgtcgtaa gatcagccag tacacccgct acggtactct ggtgctggca
    11701 atattccagt cgatcggtat tgctaccggt ctgccgaata tgcctggtat gcaaggcctg
    11761 gtgattaacc cgggctttgc attctacttc accgctgttg taagtctggt cacaggaacc
    11821 atgttcctga tgtggttggg cgaacagatt actgaacgag gtatcggcaa cggtatttca
    11881 atcattatct tcgccggtat tgtcgcggga ctcccgccag ccattgccca tactatcgag
    11941 caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt tggttgcagt attagtattt
    12001 gcagtgacgt tctttgttgt atttgttgag cgtggtcaac gccgcattgt ggtaaactac
    12061 gcgaaacgtc agcaaggtcg tcgtgtctat gctgcacaga gcacacattt accgctgaaa
    12121 gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca gtattattct gttcccggcg
    12181 accatcgcgt catggttcgg gggcggtact ggttggaact ggctgacaac aatttcgctg
    12241 tatttgcagc ctgggcaacc gctttatgtg ttactctatg cgtctgcaat catcttcttc
    12301 tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa cagcagataa cctgaagaag
    12361 tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa cggcgaagta tatcgataaa
    12421 gtaatgaccc gcctgaccct ggttggtgcg ctgtatatta cctttatctg cctgatcccg
    12481 gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg gtgggacctc actgcttatc
    12541 gttgttgtcg tgattatgga ctttatggct caagtgcaaa ctctgatgat gtccagtcag
    12601 tatgagtctg cattgaagaa ggcgaacctg aaaggctacg gccgataatt ggtcgcccga
    12661 gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc aagaaattat gccgtaactg
    12721 caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc agtgccgagc cgaagcataa
    12781 acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa agttgggttg agctggctag
    12841 attagccagc caatcttttg tatgtctgtg cgtttccatt tgagtatcct gaaaacgggc
    12901 ttttcagcat ggaacgtaca tattaaatag taggagtgca tagtggcccg tatagcaggc
    12961 attaacattc ctgatcataa gcatgccgta atcgcattaa cttcgattta tggcgtcggc
    13021 aagacccgtt ctaaagccat cctggctgca gcgggtatcg ctgaagatgt taagatcagt
    13081 gagctgtctg aaggacaaat cgacacgctg cgtgacgaag ttgccaaatt tgtcgttgaa
    13141 ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc tgatggatct tggttgctat
    13201 cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc agcgtaccaa gaccaacgca
    13261 cgtacccgta agggtccgcg caaaccgatc aagaaataat cggggtgatt gaataatggc
    13321 aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa gtctctgacg gcgtggctca
    13381 tatccatgct tctttcaaca acaccatcgt gactatcact gatcgtcagg gtaacgcgtt
    13441 gggttgggca acagccggtg gttccggttt ccgtggttct cgcaaatcca ctccgtttgc
    13501 agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa gaatacggca tcaagaatct
    13561 ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct actattcgtg ctctgaacgc
    13621 cgcaggtttc cgcatcacta acattactga tgtgactccg atccctcata acggttgtcg
    13681 tccgccgaaa aaacgtcgcg tataacgcct cgttttccag gtttgttgga gaaagaaaat
    13741 ggcaagatat ttgggtccta agctcaagct gagccgtcgt gagggcaccg acttattcct
    13801 taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt gaacaagctc ctggccagca
    13861 cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag ttgcgtgaaa agcaaaaagt
    13921 tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac tactacaaag aagcagcacg
    13981 tctgaaaggc aacaccggtg aaaacctgtt ggctctgctg gaaggtcgtc tggacaacgt
    14041 tgtataccgt atgggcttcg gtgccactcg tgcagaagca cgtcagctgg ttagccataa
    14101 agcaattatg gtaaacggtc gtgttgttaa catcgcttct tatcaggtta gtccgaatga
    14161 cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc gtgaaagccg ctctggagct
    14221 ggctgagcag cgtgaaaagc caacctggct ggaagttgat gctggcaaga tggaaggtac
    14281 gtttaagcgt aagccggagc gttctgatct gtctgcggac attaacgaac acctgatcgt
    14341 cgagctttac tccaagtaaa gcttagtacc aaagagagga cacaatgcag ggttctgtga
    14401 cagagtttct aaaaccgcgc ctggttgata tcgagcaagt gagttcgacg cacgccaagg
    14461 tgacccttga gcctttagag cgtggctttg gccatactct gggtaacgca ctgcgccgta
    14521 ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt tgagattgat ggtgtactac
    14581 atgagtacag caccaaagaa ggcgttcagg aagatatcct ggaaatcctg ctcaacctga
    14641 aagggctggc ggtgagagtt cagggcaaag atgaagttat tcttaccttg aataaatctg
    14701 gcattggccc tgtgactgca gccgatatca cccacgacgg tgatgtcgaa atcgtcaagc
    14761 cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc tattagcatg cgtatcaaag
    14821 ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat tcattcggaa gaagatgagc
    14881 gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc tgtggagcgt attgcctaca
    14941 atgttgaagc agcgcgtgta gaacagcgta ccgacctgga caagctggtc atcgaaatgg
    15001 aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg tgcggcaacc attctggctg
    15061 aacaactgga agctttcgtt gacttacgtg atgtacgtca gcctgaagtg aaagaagaga
    15121 aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga tctggaattg actgtccgct
    15181 ctgctaactg ccttaaagca gaagctatcc actatatcgg tgatctggta cagcgtaccg
    15241 aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc tcttactgag attaaagacg
    15301 tgctggcttc ccgtggactg tctctgggca tgcgcctgga aaactggcca ccggcaagca
    15361 tcgctgacga gtaaccggat cacaggttaa ggttttactg agaaggataa ggtcatgcgc
    15421 catcgtaaga gtggtcgtca actgaaccgc aacagcagcc atcgccaggc tatgttccgc
    15481 aatatggcag gttcactggt tcgtcatgaa atcatcaaga cgactctgcc taaagcgaaa
    15541 gagctgcgcc gcgtagttga gccgctgatt actcttgcca agactgatag cgttgctaat
    15601 cgtcgtctgg cattcgcccg tactcgtgat aacgagatcg tggcaaaact gtttaacgaa
    15661 ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc gtattctgaa gtgtggcttc
    15721 cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg ttgatcgttc agagaaagca
    15781 gaagctgctg cagagtaatc tgaagcaacg taaaaaaacc cgccccggcg ggttttttta
    15841 tacccgtagt atccccactt atctacaata gctgtactct ttttgttcat cccctggagt
    15901 atttatgtgg ttacttgacc agtgggcaga gcgccatata gcagaagcgc aagcgaaagg
    15961 tgagtttgat aacctggcag gtagcggcga accattgata ctggatgatg attctcacgt
    16021 gccaccggaa ttacgtgcgg ggtatcgctt gctgaagaat gccggttgct taccgccaga
    16081 acttgagcaa cggagagaag caattcagct tctggatatt ctcaaaggta tccgtcacga
    16141 tgatccgcaa tatcaagagg ttagccgtcg attgtcatta ctggaattga agctgcgaca
    16201 agctggattg agtaccgatt ttttacgcgg cgattatgct gacaagttgt tggacaaaat
    16261 caacgataac tagtggagta tgtatgtatc gcattggtga gctggcaaaa atggcggaag
    16321 taacacccga cacgattcgt tattacgaaa aacagcagat gatggagcat gaagtgcgta
    16381 ctgaaggtgg gtttcgccta tataccgaaa gcgatctcca gcgattgaaa tttatccgcc
    16441 atgccagaca actaggtttc agtctggagt cgatccgcga gttgctgtcg atccgcatcg
    16501 atcctgaaca ccatacctgt caggagtcaa aaggcattgt gcaggaaaga ttgcaggaag
    16561 ttgaagcacg gatagccgag ttgcagagta tgcagcgttc cttgcaacgc cttaacgatg
    16621 cctgttgtgg gactgctcat agcagtgttt attgttcgat tcttgaagct cttgaacaag
    16681 gggcgagtgg cgttaagagt ggttgttgat tttttgcact ggcaggatta cactcgcgcc
    16741 gttaaataac caactggagt ttttatgagt cgatatcagc atactaaagg gcagataaag
    16801 gataatgcga tagaagcatt actacatgat cccttattcc gacagcgcgt agagaaaaat
    16861 aagaagggga aaggcagtta catgcgaaaa ggaaaacatg gcaatcgggg gaactgggag
    16921 gccagtggca agaaagtaaa tcactttttt accactggtc ttctgctttc aggcgcttgt
    16981 taagagcggt tattctgctc tttcagcaaa tcacgaattt ctgtcagtaa tacttcttct
    17041 ttagttggtg caggtgcggc tgctggttct tcttttttcc gattcagttt gttgattagc
    17101 ttaatcgcca taaagatggc aaaggccaca atcagaaaat caaagacgtt ttgaatgaag
    17161 acaccgtaat gcatcacaac agcagggata tccccctgcg catcgcgtag cgtgacagca
    17221 aactgtttaa aatcgatccc gccaattaat aagcccagag gaggcatgat gatatcggca
    17281 accagtgaag agacaatctt cccgaatgcc gcaccgataa tgacacccac cgccaaatcc
    17341 accacgttcc cgcgcatcgc aaattcgcga aattctttaa taatgctcat gttattctcc
    17401 ctatgaagcc gacaaccata agtctaacaa atgttaagcc attttcctgc caggaatgaa
    17461 tttaattaat ccttgataat caaagggaaa taataaggcg tcattagacg ccttattaat
    17521 tacaagaaga aagggcttgg ctggaagagt ctttcgacgt cggtaataaa ctttttatct
    17581 gtgaggaaca taattacgtg atcgccttgc tcaatgcgca gattgtcatt ggcaatcatc
    17641 acgtcgtttc cacgtaccac cgctccaata atcgttcctg gcggtagctt gatttcgtca
    17701 atgactctgc cgacaacgcg tgaggtgctt tcatcaccgt gagcaacggc ttcaatagct
    17761 tctgctacgc cgcggcgcaa tgaggaaaca ccaacaatat ctgcttttcg cacatggcta
    17821 agcaacgcag aaatagttgc ttgttgtggt gaaatcgcaa tatcgataac gctcccctga
    17881 accagatcca cataagcgcg acgctggatc aataccatca cctttttcgc acccatacgt
    17941 ttggcaagca tggcggacat gatattggcc tcgtcatcgt tggtgacggc aataaacaga
    18001 tcaacttgat cgatatgttc ttcggccagt agttcttgat ccgacgcatc accaaaaaag
    18061 acgatcgtat tctgtaactt ttccgccagt tcggcagcgc gctgctgatt acgttcgatg
    18121 agtttaacgc tgtaatcttt ttccagacga cgcgccagcc ctgcaccgat attaccgcca
    18181 ccaaccagca tgatccgctt atacggtttt tccagtcgct gtaattcact catcaccgcg
    18241 cggatatgct gtgaagcggc aataaagaac acttcatcac cagcttcaac aatggtcgaa
    18301 ccttgcggac gaatggggcg atcgtggcgg aaaattgctg ccacacgagt atcgatatgt
    18361 ggcatatgtt cgcgcatggt cgaaagtgca ttaccaatca gcgggccgcc ataataggct
    18421 ttaaccacag ccaggctgac tttaccctca gcgaagttca ccacctgcaa tgcgccggga
    18481 tactcaatca gtcggtaaat attatcgata accaactgct ctggtgcgat cagatgatca
    18541 atcggcacag catctgaatg aaatagctta tcggcatcgc gcacgtagtc tggtgagcgg
    18601 atacgagcga tgcgattagg ggtgttgaaa agtgagtagg ctacctggca ggcaaccata
    18661 ttggtttcat ctgaactggt tacagcaacc agcatatcgg cgtcgtcggc acctgcctcc
    18721 cgcaatacgc gtggatgaga gccatgcccc tgcacgaccc gcaggtcaaa tttatcctgt
    18781 aaggtccgca gacgctcacc gttggtatcg acaacagtaa tatcgttgtt ctcgccaacc
    18841 aggttttccg ccagtgtgcc gccaacctgg ccggcaccca gaatgataat tttcatcagt
    18901 cgcgacccgt tatctcatca ctttttgatt agcttagcgt aaaagaagcc gtcgccctct
    18961 tcggcaccag gtagattttg tttacccggt tgctctggtg ttcctgtttc gcaaagttcg
    19021 gcatcagcgg tacgttgcaa aaaggcttta atctgcaggc tattctcttc cggtaacacc
    19081 gaacaggtgg catagaccag agttccaccg gtttttaaat gcggccaaat ggcgtcgaga
    19141 atttcagact gcaattgcgc gagttccggg atatcgcgat cgcgacgtaa ccatttaata
    19201 tctggatggc gacgaatcac accggttgct gaacaaggcg catctaataa aatgcgatca
    19261 aactgttgct cgccacacca ttgagaaggg taacggccat caccttgttt cacggtcgcc
    19321 ttcataccaa ggcgttttaa attgtcgtaa acccgagaga ggcgctgttc gtcgatatca
    19381 accgcaacaa cctgcgcttc tggtgccacc tcaaggatat gcgttgtttt accgccgggg
    19441 gcggcacaaa gatccaaaat gtgttcaccg ttttgtggcg caagccaggt catgcaacct
    19501 tgtgctgatg catcctgaac ggtaacccat ccgtcttcaa aaccaggtag cgcatgaaca
    19561 ggtgcaggtg tttccagacg tacagcatca gggtaatccg catgcgggaa acctttcatt
    19621 cctgcttcat ccagcaatgc aagccagctg tcgcgggaat gatgcgtacg attaatacgc
    19681 agccacattg gcggacgctg gttattggct tcgacgatgg attgccactg ctctggatac
    19741 gctttttgca gacgcttcag caaccaggaa ggatgcagat aacgtgcatc actggcatta
    19801 aactcggcta ataactcttc ttgctgacgc tggaactggc gtaatacacc gtttatcaac
    19861 cctttaagtt gcggacgctt aattgcgata gcgccttcaa ccgtttcagc cagcgcagca
    19921 tgaggtggaa tgcgggtata aagcagttga tacaaaccaa ccataatcag gtaatgcacg
    19981 gtccgctgtt tgccggtcat cggacgggcc attaacttat taatcagcca gtctaactgc
    20041 gaaagcgtac gcagtacgcc aaagcacaac tcttgaagaa gtgctttgtc tttatcggaa
    20101 actttttgct ggagcggtgg cagaatgttg cttaatgatt gcccttgctc gacgacttgt
    20161 tcaacggcct gggccgccat gctacgtaaa ttacgttgtt ttttcataag tataaaaacg
    20221 cccggcaaga ccgggcttag aagagtggac tatcagacca gacggttgcc cggaacaaac
    20281 cattcccgac gagagttcag gaggtcttgc gcgctcatcg ctttcttacc cgcaggttgt
    20341 aacgagagca ggttcaggat gccatcacca gtcgcaacct gaatgccttg tttgttggct
    20401 tcaaggatcg ttcctggtgc agcgttggtt gccgtatcaa tgaccgatgc tttccagact
    20461 ttaaccggct gtccttcaat ttccagccag ctcattggcc atggattgaa agcgcgaatg
    20521 cagcgttcaa gctgtgctgc cgaaagtgac cagtcaatac gcgcttcttc tttactcaac
    20581 ttctcggcgt aagtgacaag agtttcgtcc tgaacttctg gtttcgccgt gccgtctgcc
    20641 agttgtttca acgtggtgat aagcccttgt gggccaagct ctgccagctt gtcgtacagc
    20701 gtaccactgg tatcttctgc agtaatcggg caggagagct tatagagcat atcaccggtg
    20761 tctaaaccga catccatttg cataatggtc acaccagttt ctgcatcacc cgcccatagt
    20821 gagcgttgga ttggtgcagc accgcgccag cgtggcagca gtgaaccatg aacgttgata
    20881 cagccaagac gcggcatctc cagcactgct ttcggcagaa ttaaaccata ggcgacgacg
    20941 accataacat cagcctgcag ttcggcgacc agttgctggt tttcttgtgg acgcagggaa
    21001 acaggttgaa aaacgggcag acctttttcc tcagccagaa ctttaaccgg gctgggcatc
    21061 agttttttac cgcgtcctgc cggtcggtct ggctgggtga acacgccaac gacgttatga
    21121 ccagaagaca acagcgcgtc gagatgacgc gctgcaaagt caggtgtacc cgcaaaaata
    21181 atacgtagtg attctgacac gttagttctt atccttaagc ccgggctttc agacgatcca
    21241 gtttttcaac tttctgacga atacgttgtt gtttcagcgg tgacagataa tccataaaca
    21301 gtttgccgac caggtgatcc atctcatgct gaatacagat ggctaacaga ccgtctgctt
    21361 ccagttcaaa tggtttaccg tcgcggtcaa gggcgcgaat tttaactttc tctgcgcgcg
    21421 gcactaaagc acgttgttca gggatcgaca ggcaaccttc ttcaatgcct gtttcgccgc
    21481 ttttttctaa aagctctgga ttgattaaca ctagccgttc gtcacggttt tccgaaacat
    21541 caataacaat gatacgttga tggatatcaa cctgggttgc cgccaggcca ataccttctt
    21601 ctgcgtacat cgtctcgaac atatcatcga cgatacgctg aatttctgca ttcacttctt
    21661 ctaccggttt agcaactttg cgaagccgct cgtccggaat atgtaacact tgcaaaactg
    21721 acataaatct ccagagatgt gttcaggagt tagaaagatt atttcttcta ttctagacaa
    21781 atccccctct gattgacagc atcactgacc aatcgcaaag attgctaagg ctgcttatgg
    21841 cagggagata aggatggtcg atacagaaat ttggctgcgt ttaatgagta tcagcagctt
    21901 gtacggcgat gatatggtcc gtatcgctca ctgggtggca aaacagtcgc atattgatgc
    21961 ggttgtattg cagcaaacag ggcttacatt gcggcaggca caacgctttc tttcatttcc
    22021 acgaaagagt atcgaaagct cactttgttg gttggagcaa cccaaccatc atttaattcc
    22081 tgcggacagc gaattttatc ctcctcaact tctggcgacg acagattacc ccggcgcact
    22141 gtttgttgaa ggagaactgc acgcgctgca ttcatttcag cttgccgtag tggggagtcg
    22201 ggcgcattca tggtatggcg agcgatgggg acgattattt tgcgaaactc tggcgacgcg
    22261 tggagtgaca attacgagtg gactggcgcg tggaatcgat ggtgtagcgc ataaagcagc
    22321 cttacaggta aatggcgtca gcattgctgt attggggaat ggacttaata ccattcatcc
    22381 ccgccgtcat gcccgactgg ctgccagtct gcttgaacag gggggcgctc tcgtctcgga
    22441 atttcccctc gatgttccac cccttgctta caatttccca cgaagaaatc gcattatcag
    22501 tggtctaagt aaaggtgtac tggtggtgga agcggctttg cgtagtggtt cgctggtgac
    22561 agcacgttgt gcgcttgagc aggggcgaga agtttttgcc ttgccaggtc caatagggaa
    22621 tccgggaagc gaagggcctc actggttaat aaaacaaggt gcgattcttg tgacggaacc
    22681 ggaagaaatt ctggaaaact tgcaatttgg attgcactgg ttgccagacg cccctgaaaa
    22741 ttcattttat tcaccagatc agcaagacgt ggcattgcca tttcctgagc tcctggctaa
    22801 cgtaggagat gaggtaacac ctgttgacgt cgtcgctgaa cgtgccggcc aacctgtgcc
    22861 agaggtagtt actcaactac tcgaactgga gttagcagga tggatcgcag ctgtacccgg
    22921 cggctatgtc cgattgagga gggcatgcca tgttcgacgt actaatgtat ttgtttgaaa
    22981 cctatattca cacagaagct gagttgcgtg tggatcaaga caaacttgaa caggatctta
    23041 ccgacgcagg ttttgagcga gaagatatct acaatgccct gctatggctg gaaaaacttg
    23101 ctgattatca ggaagggttg gcagaaccga tgcaactggc ctcggatcct ctctccatgc
    23161 gtatttatac accggaagag tgtgaacgac tggatgccag ctgccgtggg tttctgcttt
    23221 tccttgagca gattcaggtg ctcaaccttg aaactcgtga aatggtgata gagcgagtgc
    23281 tcgcgctgga taacgcagag ttcgaactgg atgatctgaa atgggtgatc ctgatggtgt
    23341 tgttcaatat tccgggctgc gaaaatgcgt accagcaaat ggaagaatta ctctttgaag
    23401 tgaatgaagg tatgctgcat taatcatttc tttaattcag cataagttgt tatggcgaaa
    23461 tcagcactgt tcacggtgcg taataatgag tcctgcccaa agtgcggggc tgaactggtt
    23521 attcgatccg ggaaacacgg tccgtttctt ggatgctcac agtatccggc gtgtgactac
    23581 gtccgtcctc tgaaatcttc agcggatgga catatcgtca aagttctgga ggggcaggtt
    23641 tgccctgcat gtggcgcaaa tctggtatta cgccagggac gctttggtat gtttattggt
    23701 tgcattaact accctgaatg cgaacatacc gaacttatcg ataaaccgga cgaaacagca
    23761 attacatgcc cccaatgtcg gacgggccat ctggtccagc gccgctcccg ttatggcaaa
    23821 acatttcact cttgtgatcg ctacccggag tgtcaatttg ccattaactt caaacccata
    23881 gctggagaat gccctgagtg tcattatccg ctactcatcg aaaagaaaac cgcgcagggt
    23941 gtaaaacact tttgtgccag taaacaatgt ggaaagccgg tttcggcgga ataataacgt
    24001 gaataataac ctgcaaagag acgctatcgc agctgcgata gatgttctca atgaagaacg
    24061 tgtcatcgcc tatccaacgg aagccgtttt cggtgttggg tgcgatcctg atagcgaaac
    24121 agcagtgatg cgactgttgg agttaaaaca gcgtccggtt gataaggggc tgattttaat
    24181 cgcagcaaat tacgagcagc ttaaacccta tattgatgac accatgttga ctgacgtgca
    24241 gcgtgaaacc attttttccc gctggccagg tcctgtcacc tttgtctttc ccgcgcctgc
    24301 gacaacaccg cgctggttga cgggccgctt tgattcgctt gctgtacgag tcaccgacca
    24361 tccgttggtg gttgctttgt gccaggctta tggtaaaccg ctggtttcta ccagtgccaa
    24421 cttgagtgga ttgccacctt gtcgaacagt agacgaagtt cgcgcacaat ttggcgcggc
    24481 gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat ccttcagaaa tccgcgatgc
    24541 cctgacgggt gaactgtttc gacaggggta acataatgga aacctatgct gtttttggta
    24601 atccgatagc ccacagcaaa tcgccattca ttcatcagca atttgctcag caactgaata
    24661 ttgaacatcc ctatgggcgc gtgttggcac ccatcaatga tttcatcaac acactgaacg
    24721 ctttctttag tgctggtggt aaaggtgcga atgtgacggt gccttttaaa gaagaggctt
    24781 ttgccagagc ggatgagctt actgaacggg cagcgttggc tggtgctgtt aataccctca
    24841 tgcggttaga agatggacgc ctgctgggtg acaataccga tggtgtaggc ttgttaagcg
    24901 atctggaacg tctgtctttt atccgccctg gtttacgtat tctgcttatc ggcgctggtg
    24961 gagcatctcg cggcgtacta ctgccactcc tttccctgga ctgtgcggtg acaataacta
    25021 atcggacggt atcccgcgcg gaagagttgg ctaaattgtt tgcgcacact ggcagtattc
    25081 aggcgttgag tatggacgaa ctggaaggtc atgagtttga tctcattatt aatgcaacat
    25141 ccagtggcat cagtggtgat attccggcga tcccgtcatc gctcattcat ccaggcattt
    25201 attgctatga catgttctat cagaaaggaa aaactccttt tctggcatgg tgtgagcagc
    25261 gaggctcaaa gcgtaatgct gatggtttag gaatgctggt ggcacaggcg gctcatgcct
    25321 ttcttctctg gcacggtgtt ctgcctgacg tagaaccagt tataaagcaa ttgcaggagg
    25381 aattgtccgc gtgaatcagg ccatccagtt tccggacagg gaagagtggg acgagaataa
    25441 aaaatgtgta tgttttcccg ctctcgtgaa tggtatgcaa ctgacatgcg cgatctctgg
    25501 cgagagtctg gcgtatcgct ttactggaga tacgccagaa cagtggttag cgagttttcg
    25561 tcagcatcgc tgggacctgg aagaagaagc ggaaaactta attcaggaac aaagtgaaga
    25621 tgatcaaggc tgggtctggt taccctgatc cagatattcg tccttccatt tcacgtaatt
    25681 attcgcggaa tagcgtaacc cagccttctc ttcatcactt aacgggcgga tctgtttgac
    25741 ggggctaccg agatacagat atccgctctc cagccgttta ttttgtggga ccagactacc
    25801 cgcaccaatc atcacatcat cttctactat tgcgccatca agtaaaattg agcccatccc
    25861 aaccaaaact cgattgccaa tggtgcagcc gtggagcatc accttgtgac caacagtgac
    25921 atcttcgcca atggttaatg ggttgccatc tgggttgtac gaggatttat gagtgacatg
    25981 caacatactg ccatcctgga tattggtgcg tgctccgatc tgtacataat gtacatctcc
    26041 acgaatcaca acgagcggcc agatccccac atcatcagcc agacgaacgt caccaatcac
    26101 gacactgcta tcgtcgatca ttacgcgctg accgatttgt ggaaaaagat cgcggtatgg
    26161 gcgtaaaaca tcagacatac ttacctcagc aataaatgat ttactaatga ctttgggggc
    26221 attattggcc ttgtgcaagt cttttagtat gcaaaaaagc accgttttgt gtgcgattgc
    26281 agcaaaaagg gtgaaaaaac aacaaacaga aaaaaagatc aaaaaaatac ttgtgcaaaa
    26341 aattgggatc cctataatgc gcctccgttg agacgacaac gtgaaacact tcacaggatg
    26401 gtcggaacaa cgaagagaaa aaaatcctga aattcagggt tgactctgaa agaggaaagc
    26461 gtaatatacg ccacctcgcg acagtgagct gaaagccgcg tcgcaactgc tctttaacaa
    26521 tttatcagac aatctgtgtg ggcactcgaa gatacggatt cttaacgtcg caagacgaaa
    26581 aatgaatacc aagtctcaag agtgaacacg taattcatta cgaagtttaa ttctttgagc
    26641 atcaaacttt taaattgaag agtttgatca tggctcagat tgaacgctgg cggcaggcct
    26701 aacacatgca agtcgaacgg taacaggaag cagcttgctg tttcgctgac gagtggcgga
    26761 cgggtgagta atgtctggga aactgcctga tggaggggga taactactgg aaacggtagc
    26821 taataccgca taacgtcgca agaccaaaga gggggaccct cgggcctctt gccatcggat
    26881 gtgcccagat gggattagct tgttggtggg gtaacggctc acc
//
LOCUS       DAPHBJ010000047        26873 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.047, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000047 DAPHBJ010000000
VERSION     DAPHBJ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.047"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            3..767
                     /gene="ycaL"
                     /locus_tag="RHZ76_001765"
     CDS             3..767
                     /gene="ycaL"
                     /locus_tag="RHZ76_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HDV2812417.1"
                     /translation="MKNTKLLLAIATSAELLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            940..1623
                     /gene="cmk"
                     /locus_tag="RHZ76_001766"
     CDS             940..1623
                     /gene="cmk"
                     /locus_tag="RHZ76_001766"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDV2812418.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            1734..3407
                     /gene="rpsA"
                     /locus_tag="RHZ76_001767"
     CDS             1734..3407
                     /gene="rpsA"
                     /locus_tag="RHZ76_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDV2812419.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            3567..3851
                     /gene="ihfB"
                     /locus_tag="RHZ76_001768"
     CDS             3567..3851
                     /gene="ihfB"
                     /locus_tag="RHZ76_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HDV2812420.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            4059..6323
                     /locus_tag="RHZ76_001769"
     CDS             4059..6323
                     /locus_tag="RHZ76_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HDV2812421.1"
                     /translation="MKITTVGVCIISGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPASGLVMYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTMVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ECWQWLSFSPWFLLVVWRLNAWRTLPAMCVAGGLLMCWPLWQKPRPDEWQLYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHIWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     VDDGTNSILLTGDIEAPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQLQGYQWIDTPHQGQTTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            6360..8108
                     /gene="msbA"
                     /locus_tag="RHZ76_001770"
     CDS             6360..8108
                     /gene="msbA"
                     /locus_tag="RHZ76_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HDV2812422.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            8105..9091
                     /gene="lpxK"
                     /locus_tag="RHZ76_001771"
     CDS             8105..9091
                     /gene="lpxK"
                     /locus_tag="RHZ76_001771"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDV2812423.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            9128..10360
                     /gene="ycaQ"
                     /locus_tag="RHZ76_001772"
     CDS             9128..10360
                     /gene="ycaQ"
                     /locus_tag="RHZ76_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein YcaQ"
                     /protein_id="HDV2812424.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHDDLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGCC
                     PQGLFTDCRTGWEIDPVA"
     gene            10412..10594
                     /gene="ycaR"
                     /locus_tag="RHZ76_001773"
     CDS             10412..10594
                     /gene="ycaR"
                     /locus_tag="RHZ76_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HDV2812425.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            10591..11337
                     /gene="kdsB"
                     /locus_tag="RHZ76_001774"
     CDS             10591..11337
                     /gene="kdsB"
                     /locus_tag="RHZ76_001774"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDV2812426.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            11491..12384
                     /locus_tag="RHZ76_001775"
     CDS             11491..12384
                     /locus_tag="RHZ76_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HDV2812427.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12361..13140)
                     /gene="elyC"
                     /locus_tag="RHZ76_001776"
     CDS             complement(12361..13140)
                     /gene="elyC"
                     /locus_tag="RHZ76_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HDV2812428.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            13276..14061
                     /gene="cmoM"
                     /locus_tag="RHZ76_001777"
     CDS             13276..14061
                     /gene="cmoM"
                     /locus_tag="RHZ76_001777"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HDV2812429.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSGLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            14058..15380
                     /gene="mukF"
                     /locus_tag="RHZ76_001778"
     CDS             14058..15380
                     /gene="mukF"
                     /locus_tag="RHZ76_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HDV2812430.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            15361..16065
                     /gene="mukE"
                     /locus_tag="RHZ76_001779"
     CDS             15361..16065
                     /gene="mukE"
                     /locus_tag="RHZ76_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HDV2812431.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            16065..20525
                     /gene="mukB"
                     /locus_tag="RHZ76_001780"
     CDS             16065..20525
                     /gene="mukB"
                     /locus_tag="RHZ76_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HDV2812432.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQTDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGTDEAPSQAS"
     gene            20786..22633
                     /gene="ldtD"
                     /locus_tag="RHZ76_001781"
     CDS             20786..22633
                     /gene="ldtD"
                     /locus_tag="RHZ76_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDV2812433.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            22814..23362
                     /locus_tag="RHZ76_001782"
     CDS             22814..23362
                     /locus_tag="RHZ76_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HDV2812434.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            23389..24036
                     /gene="gloC"
                     /locus_tag="RHZ76_001783"
     CDS             23389..24036
                     /gene="gloC"
                     /locus_tag="RHZ76_001783"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HDV2812435.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVIFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(24258..25448)
                     /gene="aspC"
                     /locus_tag="RHZ76_001784"
     CDS             complement(24258..25448)
                     /gene="aspC"
                     /locus_tag="RHZ76_001784"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HDV2812436.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(25633..26721)
                     /gene="ompF"
                     /locus_tag="RHZ76_001785"
     CDS             complement(25633..26721)
                     /gene="ompF"
                     /locus_tag="RHZ76_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HDV2812437.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKDNGVNSYGGNGDKTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADIGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         6506 a         6589 c         7292 g         6486 t
ORIGIN      
        1 acatgaagaa tactaaatta ctgctggcga ttgcgacctc tgcagaatta ctgacagggt
       61 gtcaaaatac ccacggtatt gataccaata tggctatcag ctccggttta aatgcctata
      121 aagcagcaac attaagcgat gccgatgcaa aagcgattgc caatcagggc tgtgccgaaa
      181 tggacagcgg caatcaagtc gcaagtaaat ccagcaagta cggtaaacgt ctggcaaaaa
      241 tcgccaaagc attgggtaac aatattaatg gcacgccggt caactataag gtttatatga
      301 ccagcgacgt caacgcatgg gcgatggcga acggctgtgt tcgtgtctac agtggcctga
      361 tggacatgat gaatgacaac gaaattgaag gcgttctggg ccatgaactg ggccacgtcg
      421 cgttgggtca ctcgctggct gaaatgaaag cttcttatgc gatcgttgcc gcacgcgatg
      481 ccatttcagc taccagcggt gtggcttccc agctttcccg ctcacaatta ggtgatatcg
      541 cagaaggcgc tatcaatgct aaatactccc gtgataaaga gtccgaagca gatgatttct
      601 cctttgatct gttgaagaaa cgtggcatca gcacccaggg gctggttggc agctttgaaa
      661 aactggctag cctggatggc ggtcgcaccc agtccatgtt tgactctcac ccaccatcaa
      721 cagagcgtgc gcaacacatc cgtgatcgta tcgcctctgg taagtaaatc attgtcatct
      781 ttcgggctgg tcttctgcca gcccgctata attgcgcaat aaatccccat ctgaatacag
      841 acaaaactgg tttttgcaca caacgttaac gatttgtggc gtcggcgcgt ataatgcgcg
      901 cggttatgtt aacggtacgc ctgttttaag gagataaaga tgacggcaat tgccccggtt
      961 attaccattg atggcccaag cggtgcaggg aaaggcacct tgtgtaaggc tatggcggaa
     1021 gcgttgcaat ggcatctgct ggactcgggt gcaatttatc gcgtactggc attggcggca
     1081 ttacatcacc atgttgatgt tgcgtcggaa gatgcgctgg taccgctggc atcccatctg
     1141 gatgtacgtt ttgtgtcgac caatggcaat ctggaagtga tcctcgaagg ggaagatgtc
     1201 agcggcgaaa ttcgtactca ggaagtggcg aatgcagctt cacaagtcgc ggcattccca
     1261 cgcgttcgtg aagcattatt gcgtcgccaa cgcgcgtttc gcgaattacc aggtctgatt
     1321 gccgatggcc gcgacatggg aacggtggta ttccctgatg caccagtgaa aattttcctt
     1381 gacgcctcct cggaagaacg tgcgcatcgc cgcatgctac agttgcagga gaagggcttt
     1441 agtgttaact ttgagcgcct tttggccgag atcaaagaac gcgacgaccg cgatcgtaac
     1501 cgagcggtag cgccactggt tccggcagcc gatgctttag tgttggattc caccacctta
     1561 agcattgagc aagtgattga aaaagcgcta caatacgcgc gccagaaatt ggctctcgca
     1621 taagcgaccg aatttgcagt acccccgttg caatggaatg acagcgggta tgttaaacaa
     1681 ccccatccgg catggagcca ggtggacgtt aaatataaac ctgaagatta aacatgactg
     1741 aatcttttgc tcaactcttt gaagagtcct taaaagaaat cgaaacccgc ccgggttcta
     1801 tcgttcgtgg cgttgttgtt gctatcgaca aagacgtagt actggttgac gctggtctga
     1861 aatctgagtc cgccatcccg gctgagcagt tcaaaaacgc ccagggcgag ctggaaatcc
     1921 aggtaggtga cgaagttgac gttgctctgg acgcagtaga agacggcttc ggtgaaactc
     1981 tgctgtcccg tgagaaagct aaacgtcacg aagcctggat cacgctggaa aaagcttacg
     2041 aagatgctga aactgttacc ggtgttatca acggcaaagt taagggcggc ttcactgttg
     2101 agctgaacgg tattcgtgcg ttcctgccag gttctctggt agacgttcgt ccggtgcgtg
     2161 acactctgca cctggaaggc aaagagcttg aatttaaagt aatcaagctg gatcagaagc
     2221 gcaacaacgt tgttgtttct cgtcgtgccg ttatcgaatc cgaaaacagc gcagagcgcg
     2281 atcagctgct ggaaaacctg caggaaggca tggaagttaa aggtatcgtt aagaacctca
     2341 ctgactacgg tgcattcgtt gatctgggcg gcgttgacgg cctgctgcac atcactgaca
     2401 tggcctggaa acgcgttaag catccgagcg aaatcgtcaa cgtgggcgac gaaatcactg
     2461 ttaaagtgct gaagttcgac cgcgaacgta cccgtgtatc cctgggcctg aaacagctgg
     2521 gcgaagatcc gtgggtagct atcgctaaac gttatccgga aggtaccaaa ctgactggtc
     2581 gcgtgaccaa cctgaccgac tacggctgct tcgttgaaat cgaagaaggc gttgaaggcc
     2641 tggtacacgt ttccgaaatg gattggacca acaaaaacat ccacccgtcc aaagttgtta
     2701 acgttggcga tgtagtggaa gttatggttc tggatatcga cgaagaacgt cgtcgtatct
     2761 ccctgggtct gaaacagtgc aaagctaacc cgtggcagca gttcgcggaa acccacaaca
     2821 agggcgaccg tgttgaaggt aaaatcaagt ctatcactga cttcggtatc ttcatcggcc
     2881 tggacggcgg catcgacggc ctggttcacc tgtctgacat ctcctggaac gttgcaggcg
     2941 aagaagcagt tcgtgaatac aaaaaaggcg acgaaatcgc tgcagttgtt ctgcaggttg
     3001 acgcagaacg tgaacgtatc tccctgggcg ttaaacagct cgcagaagat ccgttcaaca
     3061 actgggttgc tctgaacaag aaaggcgcta tcgtaaccgg taaagtaact gcagttgacg
     3121 ctaaaggcgc aaccgtagaa ctggctgacg gcgttgaagg ttacctgcgt gcttctgaag
     3181 catcccgtga ccgcgttgaa gacgctaccc tggttctgag cgttggcgac gaagttgaag
     3241 ctaaattcac cggcgttgat cgtaaaaacc gcgcaatcag cctgtctgtt cgtgcgaaag
     3301 acgaagctga cgagaaagat gcaatcgcaa ctgttaacaa acaggaagat gcaaacttct
     3361 ccaacaacgc aatggctgaa gctttcaaag cagctaaagg cgagtaattc tctgactctt
     3421 cgggattttt attccgaagt ttgttgagtt tacttgacag attgcaggtt tcgtcctgta
     3481 atcaagcact aagggcggct acggccgccc ttaatcaatg cagcaacagc agccgcttaa
     3541 tttgccttta aggaaccgga ggaatcatga ccaagtcaga attgatagaa agacttgcca
     3601 cccagcaatc gcacattccc gccaagacgg ttgaagatgc agtaaaagag atgctggagc
     3661 atatggcctc gactcttgcg cagggcgagc gtattgaaat ccgcggtttc ggcagtttct
     3721 ctttgcacta ccgcgcacca cgtaccggac gtaatccgaa gactggcgat aaagtagaac
     3781 tggaaggaaa atacgttcct cactttaaac ctggtaaaga actgcgcgat cgcgccaata
     3841 tttacggtta agttttttac tcaaacttga acgagagaaa agcacctgtc gggtgctttt
     3901 ttcatttctc taatctggaa ctggaagctg cctcgcagag ttttgaacag ttttcaccct
     3961 ttcgttaaat tcttctgaat atgcctcggg gaacgcaaaa ttcccaccag acaaccgctc
     4021 aacaaagttg cacactttcc ataaacaggg aggggtgcat gaaaataacg acagtcggtg
     4081 tatgcataat tagcggaatt tttccgttgc tgattttgcc ccaattgcct gggacattaa
     4141 cccttgcgtt tctgactctc ttcgcctgcg tactggcatt tatccctgtt aaaaccgtcc
     4201 gttatatcgc gctgacgttg ctgtttttcg tttggggcat attatcagca aagcaaattt
     4261 tgtgggcagg agaaacctta actggcgcga cgcaggatgc aattgttgag atcactgcaa
     4321 ctgacggcat gaccactcat tacggtcaaa ttactcatct acaaggtcga cgtatattcc
     4381 ctgcgtcagg tctcgtgatg tatggcgaat atcttccgca agcggtttgt gctggacaac
     4441 aatggtcaat gaaactcaaa gttcgtgcag ttcatggcca acttaatgat ggcggctttg
     4501 atagccagcg ttatgccatt gcccagcatc agccgctcac cggccgtttt ctgcaggcaa
     4561 gtgtcattga accgaattgt agcctgcgtg cacagtatct ggcgtcacta caaacaacgc
     4621 tgcaacccta tccgtggaat gcggttattc ttggtttagg tatgggggaa cggttatccg
     4681 tccccaaaga aatcaaaaat atcatgcgcg atactggaac ggcgcattta atggcgatat
     4741 cgggattgca catcgctttt gcggcgttgc tggctgccgg actcattcgc agtggacaaa
     4801 tttttctgcc tgggcgctgg atccactggc aaataccatt aattggcgga atctgctgtg
     4861 ctgcttttta tgcctggttg acgggaatgc aacctcctgc attgcgtacc atggtggcgc
     4921 ttgctacgtg gggaatgctt aagttaagtg ggcgacagtg gagtggctgg gatgtatgga
     4981 tatgttgtct ggcggcaatt ttgctgatgg atcctgttgc cattctctcg caaagtttat
     5041 ggctctctgc cgctgcggtc gcggcattga tattttggta tcagtggttt ccctgtcctg
     5101 agtggcaact gccgccggta ttgcgtgcag ttgtttccct catccatctg caactgggaa
     5161 tcacacttct gcttatgccc gtgcaaatcg tcatatttca tggcattagt ctgacctcgt
     5221 ttattgcaaa tctattagca attcccttgg tgacatttat cacggttccg ttgatcctcg
     5281 ccgcgatggt tgtgcattta agcgggccgt taatcctgga gcaagggtta tggtttcttg
     5341 ccgaccggtc tttggcttta cttttctggg ggttaaagag tttgccggaa gggtggatca
     5401 acattgctga atgttggcaa tggctatcat tttccccatg gttcttactg gtggtatggc
     5461 gattaaacgc ctggcgaacg ttgccagcaa tgtgtgtggc tggaggcttg ctgatgtgct
     5521 ggccgctgtg gcaaaaacct cgacctgacg agtggcagct gtacatgctt gatgtcgggc
     5581 aagggctggc aatggtgata gccagaaacg gcaaagcgat tctctatgac acaggactgg
     5641 cctggcctga aggggatagt gggcaacaac tgattatccc ctggctccac tggcataatc
     5701 ttgaaccgga aggcgttatt ctgagccatg aacatctgga tcaccgggga gggctggatt
     5761 caatattgca tatatggccg atgttatgga tcagaagtcc gttaaactgg gaacatcatc
     5821 agccctgtgt gcgtggcgaa gcgtggcaat ggcaaggatt gcgtttcagc gcgcactggc
     5881 ctttacaagg tagcaacgat aaaggaaata accattcctg tgtggttaag gttgatgacg
     5941 ggacgaatag cattcttcta accggtgata ttgaagcccc agctgaacaa aagatgctaa
     6001 gccgttactg gcagcaagtg caggcaacat tgcttcaggt acctcaccat ggcagtaata
     6061 cctcatcatc gttgccatta attcagcgag tgaatggaaa agtggcactc gcatcggcat
     6121 cgcgctataa cgcatggcga ctgccctcta acaaagttaa gcatcgctat caactgcaag
     6181 gatatcaatg gattgatact ccacatcaag gtcaaacaac ggtcaatttt tcagcgcaag
     6241 gctggcggat tagcagcctc agggagcaaa ttttacctcg ttggtatcat cagtggtttg
     6301 gcgtgccagt ggataacggg tagaatatgc ggctatttca acaaatgctg gttttttgaa
     6361 tgcataacga caaagatctc tctacgtggc agacattccg ccgactgtgg ccaaccattg
     6421 cgcctttcaa agcgggtctg atcgtggcgg gcgtagcgtt aatcctcaac gcagccagcg
     6481 ataccttcat gttatcgctc cttaagccac ttcttgatga tggctttggt aaaacagatc
     6541 gctccgtgct ggtgtggatg ccgctggtgg tgatcgggct gatgatttta cgtggtatca
     6601 ccagctatgt ctccagctac tgtatctcct gggtatcagg aaaggtggta atgaccatgc
     6661 gtcgccgcct gtttggtcac atgatgggaa tgccagtttc attctttgac aaacagtcaa
     6721 cgggtacgct gttgtcacgt attacctacg attccgaaca ggttgcttct tcttcttccg
     6781 gcgcactgat tactgttgtg cgtgaaggtg cgtcgatcat cggcctgttc atcatgatgt
     6841 tctattacag ttggcaactg tcgatcattt tgattgtgct ggcaccgatt gtttcgattg
     6901 cgattcgcgt tgtatcgaag cgttttcgca acatcagtaa aaacatgcag aacaccatgg
     6961 ggcaggtgac caccagcgca gaacaaatgc tgaagggcca caaagaagta ttgattttcg
     7021 gtggtcagga agtggaaacg aaacgctttg ataaagtcag caaccgaatg cgtcttcagg
     7081 ggatgaaaat ggtttcagcc tcttccatct ctgatccgat cattcagctg atcgcctctt
     7141 tggcgctggc gtttgttctg tatgcggcga gcttcccaag tgtcatggat agcctgactg
     7201 ccggtacgat taccgttgtt ttctcttcaa tgattgcact gatgcgtccg ctgaaatcgc
     7261 tgaccaacgt taacgcccag ttccagcgcg gtatggcggc ttgtcagacg ctgtttacca
     7321 ttctggacag tgagcaggag aaagatgaag gtaagcgcgt gatcgagcgt gcgactggcg
     7381 acgtggaatt ccgcaatgtc acctttactt atccgggacg tgacgtacct gcattgcgta
     7441 acatcaacct gaaaattccg gcagggaaga cggttgctct ggttggacgc tctggttcgg
     7501 gtaaatcaac catcgccagc ctgatcacgc gtttttacga tattgatgaa ggcgaaatcc
     7561 tgatggatgg tcacgatctg cgcgagtata ccctggcgtc gttacgtaac caggttgctc
     7621 tggtgtcgca gaatgtccat ctgtttaacg atacggttgc taacaacatt gcttacgcac
     7681 ggactgaaca gtacagccgt gagcaaattg aagaagcggc gcgtatggcc tacgccatgg
     7741 acttcatcaa taagatggat aacggtctcg atacagtgat tggtgaaaac ggcgtgctgc
     7801 tctctggcgg tcagcgtcag cgtattgcta tcgctcgagc cttgttgcgt gatagcccga
     7861 ttctgattct ggacgaagct acctcggctc tggataccga atccgaacgt gcgattcagg
     7921 cggcactgga tgagttgcag aaaaaccgta cctctctggt gattgcccac cgcttgtcta
     7981 ccattgaaaa ggcagacgaa atcgtggtcg tcgaggatgg tgtcattgtg gaacgcggta
     8041 cgcataacga tttgcttgag caccgcggcg tttacgcgca acttcacaaa atgcagtttg
     8101 gccaatgatc gaaaaaatct ggtctggtga atcccctttg tggcggctat tgctgccact
     8161 ctcctggttg tatggcctgg tgagtggcgc gatccgtctt tgctataaac taaaactgaa
     8221 gcgcgcctgg cgtgcccccg taccggttgt cgtggttggt aatctcaccg caggcggcaa
     8281 cggaaaaact ccggtcgttg tctggctggt ggaacagttg caacagcgcg gtattcgcgt
     8341 gggggtcgta tcgcggggat atggtggtaa ggctgaatct tatccgctgt tattgtcggc
     8401 agataccaca acagcacagg cgggtgatga acctgtgttg atttatcaac gcactgatgc
     8461 gcctgttgcg gtttctcccg ttcgttctga tgcggtaaaa gccattctgg cgcaacaccc
     8521 tgatgtgcag atcatcgtaa ccgacgacgg tttacagcat taccgtctgg cgcgtgatgt
     8581 ggaaattgtc gttattgatg gtgtgcgtcg ctttggcaat ggctggtggt tgccggcggg
     8641 gccaatgcgt gagcgagcgg ggcgcttaaa gtcggttgat gcggtaatcg tcaacggcgg
     8701 tgtccctcgc agcggtgaaa tccccatgca tctgctgccg ggtcaggcgg tgaatttacg
     8761 taccggtacg cgttgtgacg ttgctcagct tgaacatgta gtggcgatgg cggggattgg
     8821 gcatccgccg cgcttttttg ccacgctgaa gatgtgtggc gtacaaccgg aaaaatgtgt
     8881 accgctggcc gatcatcagt ctttgaacca tgcggatgtc agtgcgttgg taagcgccgg
     8941 gcaaacgctg gtaatgactg aaaaagatgc ggtgaaatgc cgggcctttg cagaagaaaa
     9001 ttggtggtat ttgcctgtag acgcacagct ttcaggtgat gaaccagcga aactgcttac
     9061 gcaactaacc tcgctggctt ctggcaacta gttacgccgc ggcagcgttc gattgatgga
     9121 gtcatgaatg tcgctgccgc acctctccct tgctgatgcg cgtaatcttc accttgccgc
     9181 acaaggcctg ttaaacaaac cccgccgtcg agcgtcgttg gaggatattc cggcaacgat
     9241 ctcccgcatg tccttgctgc aaatcgatac catcaatatt gttgcccgta gtccatatct
     9301 ggtgcttttc agtcgtctgg gaaattatcc tgcccagtgg ctggatgagt ctctggcgcg
     9361 tggcgaatta atggaatact gggcgcatga agcctgcttt atgccgcgta gcgactttcg
     9421 tcttattcgc caccgcatgc tggcacctga aaaaatgggc tggaaataca aagacgcctg
     9481 gatgcaggaa catgaggcgg aaattgcaca gttaattcag catattcatg ataaggggcc
     9541 ggtacgttca gccgattttg agcatcctcg taaaggtgca agcggctggt gggaatggaa
     9601 gccgcataaa cggcatctgg aaggtttatt tactgccgga aaggtgatgg tgattgaacg
     9661 gcgcaacttc cagcgcgttt atgatttaac ccaccgtgtc atgcctgact gggatgatga
     9721 gcgcgatctc gtttcgcaaa cagaagcaga aatcatcatg ctggataaca gtgcgcgtag
     9781 cctgggaata ttccgcgaac agtggctggc agattactat cggctgaaac gtccggcact
     9841 ggcggcgtgg cgcgaagcga gggctgaaca gcagcaaatc attgctgtgc atgttgaaaa
     9901 attgggcaat ctttggctgc atgatgattt gctgccgcta ctcgagcgag cgctggccgg
     9961 aaagctcact gcaacgcaca gcgcggtact ttcgcctttt gatcctgttg tctgggatcg
    10021 caaacgcgca gagcagcttt ttgattttag ctaccggctg gagtgctata ccccagcgcc
    10081 gaaacgccag tatggctatt ttgttctgcc gttattacat cgtgggcaat tagttgggcg
    10141 aatggatgcc aaaatgcatc gccagacagg catccttgaa gttatctctc tgtggttaca
    10201 ggaaggtatt aaaccaacga caacgctgca aaaagggcta cgtcaggcga ttactgattt
    10261 cgctaactgg cagcaggcaa cgcgggtgac attaggatgc tgcccgcaag gcctctttac
    10321 tgattgccgc accggctggg aaatagaccc cgtcgcataa atgattatga taagatttat
    10381 gcattcataa cccgatccat ctggaggaac tatggatcat cgtctgcttg aaatcattgc
    10441 ctgcccggtt tgcaacggaa aactttggta taaccaggaa aaacaagagc ttatttgcaa
    10501 actggacaac ctcgctttcc cgctgcgtga tggcattcca gtgttgctgg aaacggaagc
    10561 ccgcgtgctg actgctgatg agagtaaatc atgagttttg tggtcattat tcccgcgcgc
    10621 tacgcgtcca cgcgtctgcc cggtaaacca ttggttgata ttaacggcaa acccatgatt
    10681 gttcatgttc ttgaacgcgc gcgtgaatca ggtgccgagc gcatcatcgt ggcaaccgat
    10741 catgaggatg ttgcccgcgc cgttgaagcc gctggcggtg aagtatgtat gacgcgcgcc
    10801 gatcatcagt caggaacaga acgtctggcg gaagttgtcg aaaaatgcgc attcagcgac
    10861 gacacggtga tcgttaatgt gcagggtgat gaaccgatga tccctgcgac aatcattcgt
    10921 caggttgctg ataacctcgc tcagcgtcag gtgggtatgg cgactctggc ggtgccaatc
    10981 cacaatgcgg aagaagcgtt taacccgaat gcggtgaaag tggttctcga cgctgaaggg
    11041 tatgcactgt acttctctcg cgccaccatt ccttgggatc gtgatcgttt tgcagaaggc
    11101 cttgaaaccg ttggcgataa cttcctgcgt catcttggta tttatggcta ccgtgcaggc
    11161 tttatccgtc gttacgtcaa ctggcagcca agtccgttag aacacatcga aatgttagag
    11221 cagcttcgtg ttctgtggta cggcgaaaaa atccatgttg ctgttgctca ggaagttcct
    11281 ggcacaggtg tggatacccc tgaagatctt gagcgcgttc gcgctgaaat gcgctaattt
    11341 cacttcacga cacttcagcc aattttggga ggagtgtcgt accgttacga ttttcctcaa
    11401 tttttctttt caacaattga tctcattcag gtgacatctt ttatattggc gctcattatg
    11461 aaagcagtag cttttatgag ggtaatctga atggaacagc tgcgtgccga attaagccat
    11521 ttactgggcg aaaaactcag tcgtattgag tgcgtcaatg aaaaagcgga tacggcgttg
    11581 tgggctttgt atgacagcca gggaaaccca atgccgttaa tggcaagaag ctttagtacg
    11641 cccggaaaag cccgacaact ggcatggaaa accaccatgc tggcaagaag tgggactgtc
    11701 cgtatgccga ctatttatgg tgtgatgacg catgaagaac accccggccc cgatgtcctg
    11761 ctactggagc ggatgcgtgg tgtttcggtg gaggcaccag cccgaacacc agaacgctgg
    11821 gaacaactca aagaccaaat cgttgaagcc ttactggcct ggcaccgtca ggacagtcgc
    11881 ggttgcgtcg gcgcggtcga caatactcag gaaaatttct ggccctcatg gtaccggcaa
    11941 catgttgaag tgctatggac cacgctcaat cagttcaata acaccggtct gacgatgcag
    12001 gataagcgga tcctgtttcg cactcgcgaa tgtctcccgg cattatttga aggctttaac
    12061 gacaattgtg tgctgattca cggtaacttc tgtttacgca gcatgttgaa agattcgcgc
    12121 agcgatcagt tactggcgat ggtcgggccg ggactaatgc tttgggcacc gcgagaatac
    12181 gaactgttcc gactaatgga taattctctg gcggaagatt tgctctggag ttacctgcaa
    12241 cgcgcgccag tggcggagtc gttcatctgg cggcgttggt tgtatgtgtt atgggatgaa
    12301 gttgcgcaac tggttaatac cggacgattt agtcggcgca acttcgatct ggcatcaaaa
    12361 tcactcttgc cgtggctcgc ctgacgaacc ttttagccac tgccagatac ggccaagcgt
    12421 ttcatagcca acacgatcgc tatgcatcaa ccagaccgga gaagggattg cccgttccca
    12481 cgggttaagc ggcgagtcga tggccaactg attcgccggg gctggtagcg gatttaaccc
    12541 ttcctgctga aaaaagatca tcgcccgcgg cagatgtgat gcggaggtca ccagcaggaa
    12601 aggggcgtca ccaatcgcct gtttcactgc tgcagcttct tcttcggtat cttttggcaa
    12661 atccagggtg ataatttgct cgcgcggcac gcccagcgat tgcgcaactc tggcacctac
    12721 ttccgctgta ctcaccgtat tggtttttgc tacgcctccc gtgaagatca gttttgatcc
    12781 aggattttcg cgccataagc gaataccttc attcaggcgt ggcagactgt tattgattaa
    12841 attagagctc ggtgcccact gcgggttcca ggtataacca cctccgagca ccacgatata
    12901 gtccactttt tgcgaattat tccatgtcgg ataggtgctt tcgatgggac gcagcaggcg
    12961 gtcggctacc ggttgcaaac tcaataacaa gagcgccagc cacccaatgc taataaaaat
    13021 cttcccggtt ttttgaaaac ggctaaacca cagcagcgcc aggccagcgc caataatcaa
    13081 caacatcagc ggaagtggca gcaacatatt gccaatcact tttttcagtg taaaaagcat
    13141 cctttctggt tcctttttta accatatagc aagggatcgc cagcgatatt acaccagacg
    13201 ggttcattct cgccgtggct gtgacaaaat agcgtcttta tcagaaaacg tcttagccaa
    13261 ccggtggaga aagcaatgca ggatcgcaat tttgatgata ttgcggaaaa gttttcccgt
    13321 aacatttacg gcaccaccaa agggcagctt cgacaggcta ttctgtggca ggatctcgat
    13381 cgcgtgctgg cggaaatggg cccgcaaaaa ctgcgtgtgc tggatgctgg cggtggagaa
    13441 gggcagaccg caatcaaaat ggccgagcgt gggcatcagg tcattttatg cgatctttct
    13501 gcgcagatga tcgaccgcgc aaaacaggcg gcagaagcaa aaggtgtgag cgacaacatg
    13561 caatttatac attgcgccgc tcaggatgtt gcttcgcatt tggaaacgcc cgttgatctg
    13621 atattgttcc atgcggtgct cgagtgggtg gccgatcccc gcagcgtatt gcagaccctg
    13681 tggtcagggt tgcgtccagg cggcgtgttg tcgttaatgt tctacaatgc gcatggtttg
    13741 ttgatgcata acatggtcgc cgggaatttt gattacgtgc aggcgggaat gccgaaaaag
    13801 aaaaaacgga cgctttcgcc agattatcca cgcgacccgg cgcaggttta tctgtggctg
    13861 gaagaagctg gttggcaaat tatgggtaag acaggcgttc gcgtgtttca tgattatctg
    13921 cgcgagaaac accagcagcg cgactgctat gaagcattac ttgaattaga aacgcgttat
    13981 tgccgtcagg aaccgtatat taccctgggg cgttatattc atgtcaccgc gcgcaaaccg
    14041 cagagcaagg ataaagtatg agtgaatttt cccagacagt ccccgaactg gttgcctggg
    14101 ccagaaaaaa tgacttctcc atctcgctgc cggtagaccg actctctttt ctgctggcgg
    14161 ttgccacgct gaacggcgag cgtctggatg gtgagatgag tgaaggcgag ctggtggatg
    14221 cattccgcca tgtgagtgat gcgtttgagc aaaccagcga aaccatcggc gtgcgcgcca
    14281 ataacgcgat caacgacatg gtgcgtcaac gtctgctgaa ccgctttacc agcgagcagg
    14341 cggaagggaa cgcaatttac cgtctgacgc cgctcggcat cggcattact gactactaca
    14401 tccgtcagcg cgagttttct acgctgcgtc tttctatgca gttgtcgatt gtggcgggtg
    14461 agctcaaacg cgcagcagat gccgccgaag agggcggtga tgaatttcac tggcaccgta
    14521 atgtctatgc gccactgaaa tattcggtag cagaaatttt cgacagtatc gacctgacgc
    14581 aacgtctgat ggacgaacag cagcagcagg tgaaggacga tatcgcccag ttgctgaaca
    14641 aagactggcg ggcggcgatt tccagctgtg aattgttgct ttcggaaact tccggaacgc
    14701 tgcgtgaatt gcaggatacg ctggaagcgg caggcgacaa attgcaggct aatctgttgc
    14761 gcattcagga tgcgacgatg acccatgacg atctgcattt cgtcgatcgt ctggtgttcg
    14821 atctgcagag caaactcgat cgtattatca gttggggcca gcaatccatc gacttgtgga
    14881 ttggctacga ccgccacgta cacaaattta ttcgtaccgc gatcgatatg gataaaaacc
    14941 gcgtctttgc tcagcggtta cgtcagtcgg tacaaaccta ttttgatgag ccgtgggcgc
    15001 taacttatgc caatgccgat cgtctgctgg atatgcgtga cgaagagatg gcactgcgcg
    15061 atgaagaagt gactggggaa cttcctgagg atctggaata cgaagagttt aacgagatcc
    15121 gcgaacagct ggcggcgatc atcgaagaac aacttgccgt gtacaaaacc agacaagtgc
    15181 cgctggatct tggtctggtg gtacgcgaat atctgtcaca gtatccgcgt gcacgtcact
    15241 ttgacgttgc gcgtattgtt attgatcagg cggtacgtct tggcgtagcg caagcagatt
    15301 tcaccggact gccagcgaaa tggcagccga ttaatgatta cggagccaag gtacaggcgc
    15361 atgtcatcga caaatattga acaagtgatg ccggttaagc tggcgcaggc gctggcgaat
    15421 ccgttatttc cggcgctgga cagcgcctta cgttcaggac gccatattgg cctcgacgaa
    15481 ctggataatc atgcattcct gatggatttt caggaatatc tggaagagtt ttacgcgcgt
    15541 tataacgttg agcttattcg cgcaccggaa gggttcttct atttacgccc acgttccacc
    15601 actcttatcc cgcgttccgt cttgtcggaa ctggatatga tggtcgggaa aatcctctgt
    15661 tatctctatc tcagcccgga acggctggcg aatgagggga ttttcaccca gcaggaactg
    15721 tacgacgaac tgctcaccct ggccgatgaa gcaaaactgc tgaaactggt gaacaaccgt
    15781 tcaaccggtt cagacgttga ccgtcagaag ttgcaggaga aagtacgttc ttcgctcaac
    15841 cgtctgcgtc gtttaggcat ggtgtggttt atgggccacg acagcagcaa gttccgcatt
    15901 accgaatcgg tgttccgctt cggagccgat gtgcgtgctg gcgacgatcc ccgtgaagca
    15961 cagcgtcgcc tgattcgtga tggcgaagca atgccgattg aaaatcatct gcaactcaac
    16021 gatgaaaccg aagagagcca gccagatagc ggagaggaag aataatgatt gaacgcggta
    16081 aatttcgctc actgacgctg attaactgga acggcttttt tgcccgaact tttgaccttg
    16141 acgagctggt cacgacgctt tccggcggta acggggcggg taaatccacc acgatggcgg
    16201 cgttcgttac ggcgctgatc cccgacctga ccctgctgca tttccgtaac actacggaag
    16261 ccggggccac cagcggttcg cgcgataaag gtctgcacgg taagctgaaa gcgggtgtct
    16321 gttattcgat gctcgacacc attaactcgc gccaccagcg cgtggtggtc ggtgtgcgtc
    16381 tgcaacaggt tgccggacgc gatcgtaaag tggatatcaa accatttgcc attcagggac
    16441 tgccgatgtc ggtgcagccg acacagctgg tgaccgaaac cctgaacgaa cgccaggcgc
    16501 gcgtgctgcc gcttaacgag ctgaaagaca agctcgaggc gatggaaggc gtgcagttta
    16561 aacagttcaa ctccattacc gattaccact cgctgatgtt cgatctgggc atcatcgcgc
    16621 gtcgtctgcg ctccgcatct gaccgtagca aattctatcg tctgatcgaa gcttcgctgt
    16681 atggcgggat ctccagtgcc attacccgtt ctctgcgcga ctacctgttg ccggaaaaca
    16741 gcggcgtgcg taaagcgttc caggacatgg aagcggcgct gcgtgaaaac cgcatgacgc
    16801 tggaagcgat tcgtgtcacc cagtcggacc gtgacctgtt taagcatctg atcagcgaag
    16861 ctaccaacta cgtggcggcg gactacatgc gtcacgccaa cgagcgccgt gtccatctcg
    16921 acaaagccct ggagtttcgt cgcgagttac atacttcgcg tcagcaactg gcggctgagc
    16981 agtacaaaca cgtcgatatg gcgcgtgagc tggcagagca caacggtgcc gaaggcgatc
    17041 tggaagcgga ttatcaggcg gccagcgatc acctgaacct ggtgcaaacc gcactgcgtc
    17101 agcaggagaa gatcgaacgc tacgaagcgg atctcgatga gctgcagatc cgtctggaag
    17161 agcaaaacga agtggtggca gaagccatcg aacgccagga agagaacgag gctcgcgcgg
    17221 aagctgccga actggaagtg gacgagctga aaagccagct tgctgactac cagcaggcgc
    17281 tggacgtcca gcaaacgcgc gcgatccagt ataaccaggc gattgctgca cttaatcgtg
    17341 ccaaagaact gtgccatctg ccggacttaa ccgccgacag cgccgccgaa tggctggaaa
    17401 ccttccaggc gaaagagctg gaagcgactg aaaaaatgct ctctcttgag cagaaaatga
    17461 gcatggcgca aaccgcgcac agccagtttg agcaggctta tcagctggtg gtggcaatca
    17521 acggcccact ggcgcgtaac gaggcgtggg atgtcgctcg cgaactattg cgcgaagggg
    17581 tcgatcagcg tcacctggca gagcaggttc agccgctgcg gatgcgatta agcgaactgg
    17641 aacagcgtct acgcgagcag caagaagctg agcgcctgct ggcagatttc tgcaaacgtc
    17701 agggcaagaa ttttgatatc gacgaactgg aagccctgca tcaggaactg gaagcacgca
    17761 ttgcctctct ttccgatagc gtgtctaacg cccgtgaaga gcgcatggca ctgcgccagg
    17821 agcaggaaca gctgcagtct cgcattcaga gtttgatgca gcgtgcgccg gtttggctgg
    17881 cagcgcaaaa cagtctcaac cagttgagcg aacagtgcgg cgaagagttt acctccagcc
    17941 aggacgtcac cgaatatctg caacagttgc tggagcgtga gcgagaggcg attgttgaac
    18001 gcgatgaagt gggcgcgcgc aaaaacgccg tcgatgaaga gatcgaacgt ttaagccagc
    18061 ctggcggctc tgaagatcag cgtctgaacg cgctggcgga gcgttttggt ggtgtgctgc
    18121 tgtcagaaat ttatgacgac gttagcctgg aagatgcgcc gtacttctca gcgctgtatg
    18181 gcccgtcacg ccacgccatc gtggtgccag atctgtcaca ggtaactgaa cacctggaag
    18241 gcttgaccga ttgcccggaa gatctctatc tgatcgaagg agatccgcag tcattcgatg
    18301 acagcgtgtt cagcgttgat gagctggaaa aagcggtagt ggtgaaaatc gccgatcgtc
    18361 agtggcgtta ttcacgtttc ccggaagtgc cgctgtttgg tcgtgctgcg cgtgaaagcc
    18421 gtattgaaag cctccatgcc gagcgtgaag tgctttccga acgcttcgcc acgctctcct
    18481 ttgatgtaca gaaaactcag cgtctgcatc aggcgttcag ccgctttatc ggcagtcatc
    18541 tggcggttgc gtttgagtct gacccggaag cagaaatccg tcaactgaac agccgtcgcg
    18601 tcgaactgga gcgggcgtta agtaatcatg aaaatgataa ccagcagcag cgtattcagt
    18661 ttgagcaggc gaaagagggc gttacggcgc tgaaccgcat tctgccgcgt ctcaacctgt
    18721 tggctgatga cagcctggcg gatcgcgtcg atgaaatccg cgaacgtctg gatgaagccc
    18781 aggaagccgc gcgttttgtt cagcagtttg gcaatcaact ggcgaaactg gaaccgattg
    18841 tttcggtatt gcagagcgac ccggaacagt tcgaacagtt aaaagaagat tacgcgtact
    18901 ctcagcagat gcagcgcgat gcccgtcagc aggcgtttgc cctgacggaa gtggtgcagc
    18961 gtcgtgcgca ctttagctat tctgactcgg cagaaatgct tagcggtaac agcgatctca
    19021 acgaaaaact gcgtgaacgt ctggaacagg cggaagcgga gcgtacccgc gctcgcgaag
    19081 cgttgcgcgg tcacgcagcg cagttgagtc agtacaacca ggtgctggct tcgctgaaaa
    19141 gttcttacga caccaaaaaa gagctactca acgatctgca acgtgaattg caggatatcg
    19201 gcgtgcgtgc tgatagcggg gcagaagagc gggcgcgtat tcgccgtgac gagctgcatg
    19261 cgcaactgag caataaccgt tcacgccgca atcaactgga aaaagcgctt accttctgcg
    19321 aagcggagat ggacaacctg acccgcaaac tgcgcaagct ggagcgggat tactttgaga
    19381 tgcgcgagca ggtagtgacc gccaaagcgg gctggtgtgc ggtgatgcgc atggtgaaag
    19441 ataacggcgt tgagcgccgc ttacaccgtc gtgagctggc ttatctctcc gctgatgatt
    19501 tgcgttccat gtcggataag gcgttaggtg cgctgcgtct ggcggtggcg gataacgaac
    19561 atctgcgcga cgtgctgcgc atgtcggaag atccgaaacg tccggagcgt aaaattcagt
    19621 tcttcgtggc ggtttatcag catctgcgtg aacgtattcg tcaggatatt attcgtaccg
    19681 atgatccggt ggaagctatc gaacagatgg agattgaact tagccgtctg accgaagaat
    19741 taacctcccg tgaacagaaa ctggcgatca gttcccgcag cgtggcgaac atcattcgca
    19801 aaaccattca gcgcgagcag aaccgtatcc gtatgctcaa ccaggggttg cagaacgtat
    19861 cgtttggtca ggtgaacagc gtgcgtctca acgtgaacgt gcgtgaaacg cacgccatgc
    19921 tactggatgt gctctctgaa cagcacgagc agcatcagga tctgtttaac agcaaccgtt
    19981 tgaccttctc ggaagcgctg gcgaaactgt atcaacgtct taacccgcag actgatatgg
    20041 ggcagcgcac gccgcagacc atcggtgaag aactgctgga ttaccgcaac tatctggaaa
    20101 tggaagttga ggttaaccgt ggttccgatg gctggctgcg cgcagagtct ggtgcattgt
    20161 cgaccggtga ggcgattggt accggtatgt cgattctggt gatggtggta caaagctggg
    20221 aagatgaatc tcgccgcctg cgcggtaaag atatctctcc ttgccgcctg ctgttcctcg
    20281 atgaagcagc gcgactggat gctcgttcta tcgccacgct gtttgaattg tgtgagcgtt
    20341 tgcaaatgca actcatcatc gcagcgccgg aaaatatcag cccggagaaa ggcaccacct
    20401 ataaactggt gcgtaaagtc ttccagaata ccgaacacgt tcatgtcgtc ggcctgcgag
    20461 gatttgcgcc gcaactccct gaaacgcttc caggaactga cgaagcgcct tctcaggcga
    20521 gttaaaatta aggcggcagc aatgccgcct tttctttttc cgaaaactcc gtttctgcac
    20581 taaaaaagtg gcacattacg gcgcgctttt ctttaaactt ctttacatta ggttatgtaa
    20641 aaacgtatcg gcgtttatat actgaagata agcctgatga gtaacaggct tgctcgtcat
    20701 actttcgtga gtattggcgt tgtacaggca agtcgtaaaa taacagcctg gctattcaga
    20761 gtatgataaa aacagggggc aagggatgtt gcttaatatg atgtgtggtc gtcggctgtc
    20821 ggcaatcagt ttgtgcctgg ccgtaacatt cgctccactg ttcaatgcgc aggccgatga
    20881 gcctgaagta atccctggcg acagcccggt ggctgtcagt gaacagggcg aggcactacc
    20941 gcaggcgcaa gccacggcaa taatggcggg gatccagcca ttgcctgaag gtgcggcaga
    21001 aaaagcccgc acgcaaatcg aatctcaatt acccgcaggt tacaagccgg tttatcttaa
    21061 ccagcttcaa ctgttgtatg ccgcacgcga tatgcaaccc atgtgggaaa accgtgatgc
    21121 tgttaaagcc ttccagcaac agctggcaga ggtggcgatt gccggtttcc agccgcagtt
    21181 taataaatgg gtagagttac tgaccgatcc tggtgttaac gggatggcac gcgacgtggt
    21241 gctctctgat gcgatgatgg gctatctcca tttcattgca aatattccgg tcaaaggcac
    21301 tcgctggcta tatagcagta aaccttatgc acttgcaacg ccgccgctct cggtgattaa
    21361 ccaatggcag ctggcgctgg ataaaggtca attgcctacg tttgttgcag gactggcacc
    21421 gcagcatccg caatatgcgg cgatgcatga atcgttactg gccttactca gtgacaccaa
    21481 accgtggccc caactgaccg gcaaagcaac gttgcgccca gggcagtgga gtaacgacgt
    21541 accggcgttg cgcgaaatat tgcaacgcac aggcatgttg gacggggggc cgaaaattac
    21601 tctacctggc gatgacacgc caactgacgc ggtagtcagc ccatccgctg ttactgttga
    21661 aacagcagaa actaagccga tggataagca aacgacgtct cgtagtaaac ctgcgcctgc
    21721 cgttcgcgcc gcctacgata atgaactggt ggaagccgtt aaacgttttc aggcatggca
    21781 aggattgggg gcagatggtg ctattggccc ggcaacacgt gactggttaa acgtaacgcc
    21841 cgcccagcgt gctggcgtgt tggctctcaa catccagcga ttgcgcttgc tgccaacaga
    21901 gctttctacc gggatcatgg ttaacattcc ggcctattcg ctggtctact atcagaacgg
    21961 caatcaggtg ctggattcgc gagtcattgt cggtcgcccc gatcgcaaaa cgccgatgat
    22021 gagcagtgcc cttaacaacg tagtggtaaa cccgccgtgg aacgtacctc caactctggc
    22081 acgcaaagat attctgccaa aagtgcgcaa cgatccggga tatctcgaaa gccatggcta
    22141 tacggtgatg cgcggctgga acagcagaga agcgattgac ccatggcagg ttgactggtc
    22201 tacaatcacg gcctcgaatt taccgttccg cttccagcag gctccaggcc cacggaactc
    22261 gctggggcgc tataaattca atatgccgag ttcagaggcc atttatttgc atgacacgcc
    22321 gaaccacaat ctgttcaagc gtgatacacg cgcattgagc tcaggctgtg tacgagtgaa
    22381 taaagcttcc gatctggcga atatgctgtt gcaggatgca ggctggaatg acaaacgtat
    22441 ttctgatgcg ctgaagcagg gtgatacacg ttacgtcaat attcggcagt cgattccggt
    22501 gaatctctac tacctgacgg cctttgttgg tgcagatggt cgtacccagt atcgtacaga
    22561 tatttacaat tatgatctgc ctgcgcgatc cagctcgcaa atcgtatcga aagcggaaca
    22621 attaatcagg taaatgaagt agttcgggga aatcagttgt cgtaataatt agcatgattg
    22681 ggggcgattc tctgcagccc ccgtcactgc tggggttgag tcatcttgac gtctgcttta
    22741 cgggcggtta aggttcctct tgtgcgccag aagtgcatat aaacgataac attgacctgt
    22801 agacttgatt atcatggaca aattcgacgc taatcgccgc aaattgctgg cgcttggtgg
    22861 cgttgcactc ggtgccgcca tcctgccgac ccctgcgttt gcaacactct ctaccccacg
    22921 cccgcgcatt ttgacactca ataatcttca taccggagag tcaatcaaag cggagttttt
    22981 cgatggcaga ggctatattc aggaagaatt ggcaaaactt aaccattttt tccgcgatta
    23041 ccgcgcgaac aaaataaagt ccatcgaccc aggattattc gaccagttgt atcgcctgca
    23101 agggttgtta ggcacgcgca aaccggtgca actcatttcc ggttatcgtt ctattgatac
    23161 caacaatgaa ctacgcgccc gcagccgtgg agtagcgaag aaaagctatc acactaaagg
    23221 ccaggcgatg gatttccata ttgaaggtat cgcgttaagc aatattcgca aagccgcgtt
    23281 atctatgcgc gcaggtggtg taggatatta tccacgtagt aactttgtgc atattgatac
    23341 cgggccagca cggcactggt agtaattgct taacgaaaca ggggcagtat gaactatcgt
    23401 attattccgg tcaccgcatt ctcccagaac tgttcattaa tctggtgtga acaaacccgt
    23461 ctggccgcac tggtcgatcc tggcggcgat gcggaaaaaa tcaaacagga agttgatgac
    23521 agcggcctga cactgatgca gatcctgctg acgcatggtc atctggacca cgttggcgca
    23581 gcggcggaac tggcgcaaca ttacggcgtg ccggttttcg gcccggaaaa agaagatgag
    23641 ttctggctgc aaggcttgcc tgcgcaaagt cgtatgtttg gtctggaaga gtgccagccg
    23701 ctgacgccag atcgttggct gaacgaaggc gataccatca gcatagggaa tgtgacttta
    23761 caggtgttac attgccctgg gcatacgccg ggtcatgtcg tgttttttga tgatcgggca
    23821 aagctgctga tttctggcga tgttattttc aaaggcggag tagggcgcag tgacttcccg
    23881 cgtggcgatc ataatcaact gatttcttca atcaaagata aattgctgcc actgggggat
    23941 gacgtgatat ttattccggg tcacggacca ttatccacac ttggttatga acgcctgcat
    24001 aatcccttcc tgcaagacga aatgcccgtc tggtaaggca cataaaaaag cccgctttta
    24061 atgcgggcct ggatttctgg caaagtgcgc tttgtttatg ccggatgcgg cacgagcgcc
    24121 ttatccggcc tacaaaatcg tgcaaattca aaatattgca ggggacgcgt aggcctgata
    24181 agcgtagcgc atcaggcaat gttgcgtttg tcatcagtct cagcccgctt ttcagcgggc
    24241 ttcattgttt ttaatgctta cagcactgcc acaatcgctt cgcacagcgg agccatgtta
    24301 tctggtgtca tcccggccac atttacgcga ccagaagcaa ccgcatatac gccaaactct
    24361 tcgcgcagac gcagcacttg ttcttttgtc aggccactga aggagaacat gccgttctgt
    24421 ttgatgataa agctgaagtc gcggtttgcg cctttttcct gcagcgtatt gacgaacaac
    24481 tgacgcatac gctgaatacg ctggcgcata tcagtcagct cttgttccca aatcgcacgt
    24541 aacgcatcgt tgctcaggat ggtggcaaca acagaagcgc cgtgtgctgg tgggttagag
    24601 tagttagcgc gaatcgccgc tttcatttgg ctgaatgcgc gatcaacggt ttcactgtcg
    24661 gcagcaacca gagtacaagc gccaacacgc tcgttgtaca ggccaaagtt tttagagtag
    24721 gaactggcaa caatcagctc tttatgcata gccgcgaaag cgcgcagtcc ttcagcatct
    24781 tcttccagac cacgggcaaa accctggtaa gcgaagtcaa acagcggtaa ccagcctttc
    24841 tcaacggaga gttgtgccag tgtctgccat tgttccagcg tagggtcgat accggttggg
    24901 ttatggcagc agccatggaa cagcactacg tcgccagcct gagcttcatt caggctgtta
    24961 atcagtgcat cgaagtcaag agtgtgattt tccgcatcat aataagcgta ttcacgaact
    25021 tccagacctg cagagttaaa gacgctctta tggttcggcc agcttgggtt gctcacccac
    25081 actcgcttaa cgctggtatt ttttgccagg aaatcggcag ctacgcgtag tgcgccagtg
    25141 ccccccggag tctgtgccgt gcgagcacgt ttgtcattga tcagggcgct acctttacca
    25201 aacagcagtt cctgagtgca gcgaccaaat tcagggatgc cgtcaatgcc gaggtaattt
    25261 ttggtggttt cattttcgag cagatactgt tcagcctttt tcacgctggt cagtaccggg
    25321 gttttgcccg tctcatcttt atagacacca atcccgaggt taattttgcc gggacgttca
    25381 tcggcacgaa acagatcggc caggcccaga atcgggtcgg caggagcggc ggtaatgttc
    25441 tcaaacatga cgaggttcca ttatggttac agaagggaag tccgctatca gggtaacggg
    25501 agatttacaa aattccaact attactgatg aaaactcagg ctgtttttgc aagacgtgag
    25561 attgctctgg aaggtataaa aaaaacagga ccaacgtcct gttttttcgg catttaacaa
    25621 agaggtgtgc tattagaact ggtaaacgat acccacagca acggtgtcgt ctgaacctac
    25681 gcccagtttg ttgtcagaat cgatctggtt gatgatgtag tcaacatagg tggacatgtt
    25741 tttgttgaag tagtaggttg cgcccacttc aaagtagttc accagatcaa catcaccgat
    25801 accttctacg tctttcgctt tagatttggt gtaagcgatg gacggacgca gaccgaaatc
    25861 gaactggtat tgcgcaacta acagaacgtc ttgcgttttg ttggcgaagc cgctggtgtt
    25921 tgtaaattta ttagtgatcg gcgtagcgtt acgggtttca ccgtagttcg ctgccaggta
    25981 gatgttgttc gcgtcgtact tcagaccagt agcccactgt tcagcttttt taccgttgcc
    26041 aagaggttga gcttcttgca ggttggtacg gtcagctgca ccataagcac caacgatacc
    26101 aaagccttcg tattcgtagc tgatagaacc gccaacaccg tcgccgttag agcggcgtgc
    26161 agtgtcacgc tcgtttttac ccaggtactg aacagcgaag ttcaggccat caaccagacc
    26221 aaagaagttg gagttacgat aggtagcaac gccgccaaca cgaccaacga agaagtcatc
    26281 gctgtatgca gtatcaccac caaattctgg cagcatatcg gtgtaaccca gtgcatcata
    26341 aaccacaccg tagttacggc cgtaatcgaa agaaccaatg tcagcgtatt taagacccgc
    26401 gaatgccaga cgcgttttgt taccagtttg agcgtcagcg ccttcagagt tgttaccctg
    26461 gaagttatat tcccactgac cataaccggt cagatcggaa ttgatttgtg tttccccttt
    26521 aaaaccaaga cgggcataag ttttgtcgcc gtttccgccg taactgttta caccattgtc
    26581 tttagaaaaa taatgcagac cgacagcttt accgtacaga tctactttgt tgccatcttt
    26641 gttatagatt tctgcagcgt ttgcagtacc tgctactaac agagcaggga cgatcactgc
    26701 cagaatattg cgcttcatca ttatttatta ccctcatggt tttttttatg acacctgcca
    26761 ctgccgtcaa taagttctgt caataaaaat ttacggaact attgatgaga gtttggtgtc
    26821 tttatgtgtc tgcaggcatc tttccattca aactaacgtt tcgctaccgt gaa
//
LOCUS       DAPHBJ010000048        26117 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.048, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000048 DAPHBJ010000000
VERSION     DAPHBJ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.048"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(39..1289)
                     /gene="icd"
                     /locus_tag="RHZ76_001786"
     CDS             complement(39..1289)
                     /gene="icd"
                     /locus_tag="RHZ76_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDV2812438.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            1461..2114
                     /gene="rluE"
                     /locus_tag="RHZ76_001787"
     CDS             1461..2114
                     /gene="rluE"
                     /locus_tag="RHZ76_001787"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HDV2812439.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2124..2585
                     /gene="nudJ"
                     /locus_tag="RHZ76_001788"
     CDS             2124..2585
                     /gene="nudJ"
                     /locus_tag="RHZ76_001788"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HDV2812440.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2639..3745
                     /gene="mnmA"
                     /locus_tag="RHZ76_001789"
     CDS             2639..3745
                     /gene="mnmA"
                     /locus_tag="RHZ76_001789"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDV2812441.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3781..4422
                     /gene="hflD"
                     /locus_tag="RHZ76_001790"
     CDS             3781..4422
                     /gene="hflD"
                     /locus_tag="RHZ76_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HDV2812442.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4426..5796
                     /gene="purB"
                     /locus_tag="RHZ76_001791"
     CDS             4426..5796
                     /gene="purB"
                     /locus_tag="RHZ76_001791"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDV2812443.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5965..6636
                     /gene="phoP"
                     /locus_tag="RHZ76_001792"
     CDS             5965..6636
                     /gene="phoP"
                     /locus_tag="RHZ76_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HDV2812444.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHI
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6636..8096
                     /gene="phoQ"
                     /locus_tag="RHZ76_001793"
     CDS             6636..8096
                     /gene="phoQ"
                     /locus_tag="RHZ76_001793"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HDV2812445.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8172..9293
                     /gene="roxA"
                     /locus_tag="RHZ76_001794"
                     /gene_synonym="ycfD"
     CDS             8172..9293
                     /gene="roxA"
                     /locus_tag="RHZ76_001794"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HDV2812446.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9342..10568)
                     /gene="pepT"
                     /locus_tag="RHZ76_001795"
     CDS             complement(9342..10568)
                     /gene="pepT"
                     /locus_tag="RHZ76_001795"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDV2812447.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAHLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            10818..11954
                     /gene="potA"
                     /locus_tag="RHZ76_001796"
     CDS             10818..11954
                     /gene="potA"
                     /locus_tag="RHZ76_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HDV2812448.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            11938..12795
                     /gene="potB"
                     /locus_tag="RHZ76_001797"
     CDS             11938..12795
                     /gene="potB"
                     /locus_tag="RHZ76_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HDV2812449.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            12792..13586
                     /gene="potC"
                     /locus_tag="RHZ76_001798"
     CDS             12792..13586
                     /gene="potC"
                     /locus_tag="RHZ76_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HDV2812450.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13583..14629
                     /gene="potD"
                     /locus_tag="RHZ76_001799"
     CDS             13583..14629
                     /gene="potD"
                     /locus_tag="RHZ76_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HDV2812451.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(14766..15605)
                     /gene="cobB"
                     /locus_tag="RHZ76_001800"
     CDS             complement(14766..15605)
                     /gene="cobB"
                     /locus_tag="RHZ76_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415638.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDV2812452.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGLKAG
                     SIA"
     gene            complement(15621..16532)
                     /gene="nagK"
                     /locus_tag="RHZ76_001801"
     CDS             complement(15621..16532)
                     /gene="nagK"
                     /locus_tag="RHZ76_001801"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDV2812453.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITAQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(16561..17805)
                     /gene="lolE"
                     /locus_tag="RHZ76_001802"
     CDS             complement(16561..17805)
                     /gene="lolE"
                     /locus_tag="RHZ76_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HDV2812454.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LMESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(17805..18506)
                     /gene="lolD"
                     /locus_tag="RHZ76_001803"
     CDS             complement(17805..18506)
                     /gene="lolD"
                     /locus_tag="RHZ76_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HDV2812455.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(18499..19698)
                     /gene="lolC"
                     /locus_tag="RHZ76_001804"
     CDS             complement(18499..19698)
                     /gene="lolC"
                     /locus_tag="RHZ76_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HDV2812456.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFSRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNRQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            19960..21033
                     /locus_tag="RHZ76_001805"
     CDS             19960..21033
                     /locus_tag="RHZ76_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDV2812457.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWSIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALLLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            21161..24607
                     /gene="mfd"
                     /locus_tag="RHZ76_001806"
     CDS             21161..24607
                     /gene="mfd"
                     /locus_tag="RHZ76_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDV2812458.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            24751..25713
                     /gene="ldtC"
                     /locus_tag="RHZ76_001807"
     CDS             24751..25713
                     /gene="ldtC"
                     /locus_tag="RHZ76_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HDV2812459.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(25796..26053)
                     /gene="bhsA"
                     /locus_tag="RHZ76_001808"
     CDS             complement(25796..26053)
                     /gene="bhsA"
                     /locus_tag="RHZ76_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HDV2812460.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQMAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         6313 a         6832 c         6659 g         6313 t
ORIGIN      
        1 tgttatacgc ccgtttcaat atttaacaca tggagagatt acatgttttc gatgatcgct
       61 tcaccaaact ctgaacattt cagcagctta gcgccttcca tcagacgttc gaagtcataa
      121 gttacggtct tggcattgat tgcgccttcc atacctttaa caatcaggtc agccgcttca
      181 gtccaaccca tatggcgcag catcatctca gcggagagga tgatagagcc tggatttact
      241 ttgtcctgac cggcatattt cggcgcagta ccgtgggtgg cttcaaacag ggcgcattcg
      301 tcaccgatgt ttgcaccagg ggcgataccg ataccgccaa cctgcgctgc caaggcgtca
      361 gaaatgtagt caccgttcag gttcatacag gcgataacat catattcagc cggacgcagc
      421 aggatctgtt gcaggaatgc atcagcgatc acgtctttaa tgacgatctc tttgccagtg
      481 ttcgggtttt taactttcag ccacgggcca ccgtcgatca gttcaccgcc aaactcttca
      541 cgcgccagct ggtagcccca gtctttaaac gctccttcgg tgaacttcat gatgttgcct
      601 ttgtgcacca gagtcacaga gtcacgatcg ttagcaattg cgtattcgat cgctgcacga
      661 accagacgtt tggtgccttc ttccgaacac ggcttaatac cgataccaca atgttccggg
      721 aagcgaattt tcttcacccc catctcttca cgcaggaatt taatcacttt ctcggcgtcg
      781 gcagagtctg ctttccattc gatacccgca taaatgtctt ccgagttttc acggaagata
      841 accatatcgg tcagttcagg gtgtttaacc gggcttggag tgccctgata gtaacgtacc
      901 ggacgcaggc agatgtagag atccagttcc tggcgcaggg caacgttcag agagcgaata
      961 ccgccaccaa caggagtggt cagcggacct ttaatggcaa cgcgatattc acgaatcaga
     1021 tcaagggttt cagcaggcag ccagacatcc tgaccataaa cctgtgtgga tttttcaccg
     1081 gtgtaaattt ccatccagga gattttacgc tcgcctttat aggctttctc gactgcagcg
     1141 tcgaccactt tcagcatggc tggggttaca tctacaccga ttccatcacc ttcaatgtaa
     1201 gggataatcg gattttcagg aacgttgagt ttgccgtttt gcagggtgat cttcttgcct
     1261 tgtgccggaa caactacttt actttccatt cacctctcct tcgagcgcta ctggtttgct
     1321 cgtctgccac cacgttgcat atgcgtttgc gtcctgcgat acggatgctt tagagcaatt
     1381 ttttgttaat gatttgtaat tggcttgtca atactaccct attgtttgcc gtcatgaaag
     1441 atgcgcgtta ttcggttata atgcggcaat tcataatctc tgaaaatacc atgcaaaaaa
     1501 cttcttttag aaatcaccag gttaagcgat tcagctcgca acgttctacc aggcgtaaac
     1561 ctgaaaacca gcccacgcgt gtgatcctct tcaataaacc ctacgatgtt cttccgcagt
     1621 tcaccgatga agccggacgc aaaacattaa aagaattcat cccggttcag ggcgtttatg
     1681 cagcaggtcg ccttgaccgc gatagcgaag ggttgctggt gctgaccaat aacggcgcgt
     1741 tgcaggcgcg tttaacccag ccgggtaaac gcaccggaaa aatctattat gtgcaggtgg
     1801 aaggtattcc cacacaagac gcacttgaag ccttgcgcaa tggcgtaacc ttaaatgatg
     1861 gccctaccct gcccgcaggc gcggagctgg ttgacgaacc cgcgtggtta tggccgcgga
     1921 atccaccaat tcgtgaacgc aaaagtattc ccaccagctg gctgaagatc accttatatg
     1981 aaggacgtaa tcgccaggtg cgccgcatga ccgcccatgt tggtttccct acgctgcgac
     2041 tgattcgcta tgcgatggga gattactctt tggataatct tgccaatggc gaatggcgag
     2101 aagtgacaga ttaaggatgc aaaatgttta aaccgcacgt taccgttgct tgcgtggtgc
     2161 acgcagaagg caaattttta gtcgttgaag agacgattaa tggtaaagcg ttatggaacc
     2221 aacctgccgg gcatctggaa gccgatgaaa ccttagtgga agccgccgcc cgtgaactgt
     2281 gggaagaaac cggcatcagc gcgcagccgc aacactttat tcgtatgcat cagtggattg
     2341 cgccagataa aacgccgttt ttgcgtttcc tctttgccat tgagcttgag caaatatgcc
     2401 cgacccagcc tcatgacagc gatatcgact gctgccgttg ggtcagcgcc gaagaaattt
     2461 tacaggcgtc aaatcttcgt tcgccgctgg tggcggaaag tattcgttgt tatcaaagcg
     2521 ggcaacgtta tccgctggag atgattggcg attttaactg gccttttaca aagggtgtca
     2581 tctaaaaggc tgcgtgatag aatacgccgc cttgaagttc aatgtcgtga gtgatccaat
     2641 gtctgaaacc gcaaaaaaag taatcgtcgg catgtccggc ggtgtcgatt cctccgtttc
     2701 tgcctggctg ttgcaacaac agggatatca ggtcgaaggc ctgtttatga agaactggga
     2761 agaagacgac ggtgaggaat attgcacagc ggcagcggat ctggctgatg cccaggctgt
     2821 ctgcgacaag ctcggcattg aactgcacac cgttaacttt gctgccgagt actgggacaa
     2881 cgtcttcgaa ctgttccttg ccgaatataa agccggtcgc acgccgaatc cggatattct
     2941 gtgcaacaaa gagatcaaat ttaaagcctt cctcgaattt gccgccgaag atttaggtgc
     3001 cgattatatc gctaccggtc attacgtacg tcgggccgat gtcgatggca agagccgcct
     3061 gctgcgtggt ctggacagca ataaagacca gagctacttc ctttatacgc tcagccatga
     3121 gcagattgcg caaagcctgt tcccggtcgg cgaactggaa aaaccgcagg tgcgtaagat
     3181 tgctgaagat cttggtctgg tcaccgcgaa gaaaaaagac tctaccggca tttgcttcat
     3241 tggcgaacgt aaattccgcg agttcctggg ccgttatctc ccggcgcaac cgggcaaaat
     3301 cattaccgtc gatggcgatg aaattggtga gcaccagggg ctgatgtatc acactctcgg
     3361 tcagcgtaaa ggtctgggta tcggtggcac caaagaaggt accgaagaac cgtggtatgt
     3421 ggtggacaaa gacgtcgaaa acaacattct ggttgtcgct cagggccatg aacacccgcg
     3481 gctgatgtct gtcgggttga ttgcccagca gttgcactgg gtcgatcgcg aaccattcac
     3541 cggcactatg cgttgcacgg taaaaacccg ctatcgccag accgatatcc cttgcaccgt
     3601 caaggcgctg gacgatgatc gcattgaagt gattttcgat gaaccggttg ccgccgtgac
     3661 gccgggccag tctgccgtct tctataacgg tgaagtgtgc ctcggtggcg gtattattga
     3721 gcagcgtctg ccgctgccgg tctgattatt atctttactt aaaacaagga agcagtgaac
     3781 gtggcaaaga attactatga catcaccctc gccctggccg gtatttgtca gtcggcacgc
     3841 ctggtgcaac aactcgctca ccaggggcat tgtgatgccg atgcgctaca cgtctcactc
     3901 aacagtatta ttgatatgaa ccccagctcg acgctggcgg tttttggcgg tagcgaagcc
     3961 aacctgcgcg tcgggctgga aaccctgctc ggcgtgctca atgccagcag tcgccagggc
     4021 ttaaacgccg aattaacccg ctacacactc agcttgatgg tgcttgagcg caaactctcc
     4081 tcagcgaaag gcgcgctcga cactctgggc aaccggatca acggcctgca acgccagctc
     4141 gaacacttcg atttacagtc cgaaacgctg atgagcgcga tggctgctat ctatgttgat
     4201 gtgattagcc cgcttggccc gcgcattcag gtcaccggtt cccctgctgt actgcaaagc
     4261 ccacaagtgc aggcgaaagt tcgcgcaacc ctgctggcag gcattcgcgc cgccgtgctc
     4321 tggcaccagg tcggcggcgg acgtctgcaa ctgatgtttt ctcgtaatcg cctgaccact
     4381 caggcaaaac aaattcttgc tcatttaacc ccggagttgt gatctatgga attatcctca
     4441 ctgaccgccg tttcccctgt cgatggacgc tacggcgata aagtcagcgc gctgcgcggg
     4501 attttcagcg aatatggttt gctgaaattc cgtgtacaag ttgaagtacg ttggctgcaa
     4561 aaactggccg cgcacgcagc gatcaaggaa gttcctgctt ttgctgccga cgcaatcggt
     4621 taccttgatg caatcgtcgc cagtttcagc gaagaagatg cggcgcgcat caaaactatc
     4681 gagcgtacca ctaaccacga cgttaaagcg gttgagtatt tcctgaaaga aaaagtggcg
     4741 gagatcccgg aactgcacgc ggtttctgaa ttcatccact ttgcctgtac ttcggaagat
     4801 atcaataacc tctcccacgc attaatgctg aaaaccgcgc gtgatgaagt gatcctgcca
     4861 tactggcgtc aactgattga tggcattaaa gatctcgccg ttcagtatcg cgatatcccg
     4921 ctgctgtctc gtacccacgg tcagccagcc acgccgtcaa ccatcggtaa agagatggca
     4981 aacgtcgcct accgtatgga gcgccagtac cgccagctta accaggtgga gatcctcggc
     5041 aaaatcaacg gcgcggtcgg taactataac gcccacatcg ccgcttaccc ggaagttgac
     5101 tggcatcagt tcagcgaaga gttcgtcacc tcgctgggta ttcagtggaa cccgtacacc
     5161 acccagatcg aaccgcacga ctacattgcc gaactgtttg attgcgttgc gcgcttcaac
     5221 actattctga tcgactttga ccgtgacgtc tggggttata tcgcccttaa ccacttcaaa
     5281 cagaaaacca ttgctggtga gattggttct tccaccatgc cgcataaagt taacccgatc
     5341 gacttcgaaa actccgaagg gaatctgggc ctttccaacg cggtattgca gcatctggca
     5401 agcaaactgc cggtttcccg ctggcagcgt gacctgaccg actctaccgt gctgcgtaac
     5461 ctcggcgtgg gtatcggtta tgccttgatt gcatatcaat ccaccctgaa aggcgtgagc
     5521 aaactggaag tgaaccgtga ccatctgctg gatgaactgg atcacaactg ggaagtgctg
     5581 gctgaaccaa tccagacagt tatgcgtcgc tatggcatcg aaaaaccgta cgagaagctg
     5641 aaagagctga ctcgcggtaa gcgcgttgac gccgaaggca tgaagcagtt tatcgatggt
     5701 ctggcgttgc cagaagaaga gaaagcccgc ctgaaagcga tgacgccggc taactatatt
     5761 ggtcgagcta tcacgatggt tgatgagctg aaataaacct cgtatcagtg ccggatggcg
     5821 atgctgtccg gcctgcttat taagattatc cgctttttat tttttcactt tacctcccct
     5881 ccccgctggt ttatttaatg tttaccccca taaccacata atcgcgttac actattttaa
     5941 taattaagac agggagaaat aaaaatgcgc gtactggttg ttgaagacaa tgcgttgtta
     6001 cgtcaccacc ttaaagttca gattcaggat gctggtcatc aggtcgatga cgcagaagat
     6061 gccaaagaag ccgattatta tctcaatgaa catataccgg atattgcgat tgtcgatctc
     6121 ggattgccag acgaggacgg tctgtcactg attcgccgct ggcgtagcaa cgatgtttca
     6181 ctgccgattc tggtattaac cgcccgtgaa agctggcagg acaaagtcga agtattaagt
     6241 gccggtgctg atgattatgt gactaaaccg tttcatattg aagaggtgat ggcgcgaatg
     6301 caggcattaa tgcggcgtaa tagcggtctg gcttcacagg tcatttcgct ccccccgttt
     6361 caggttgatc tctctcgccg tgaattatct attaatgacg aagtgatcaa actgaccgcg
     6421 ttcgaataca ccattatgga aacgttgata cgcaataatg gcaaagtggt cagcaaagat
     6481 tcgttaatgc tccaactcta tccggatgcg gagctgcggg aaagccatac cattgatgta
     6541 ctgatgggac gtctgcgcaa aaaaattcag gcacaatatc cccaagaagt gattaccacc
     6601 gttcgcggcc agggctatct gttcgaattg cgctgatgaa aaaattactg cgtctttttt
     6661 tcccgctctc gctgcgggta cgttttctgt tggcaacggc agcggtagta ctggtgcttt
     6721 cgcttgccta cggaatggtc gcgctgatcg gttatagcgt cagtttcgat aaaactacgt
     6781 ttcggctgtt acgtggcgag agcaatctgt tctataccct tgcgaagtgg gaaaacaata
     6841 agttgcatgt cgagttaccc gaaaatatcg acaagcaaag ccccaccatg acgctaattt
     6901 atgatgagaa cgggcagctt ttatgggcgc aacgtgacgt gccctggctg atgaagatga
     6961 tccagcctga ctggctgaaa tcgaatggtt ttcatgaaat tgaagcggat gttaacgata
     7021 ccagcctctt gctgagtgga gatcattcga tacagcaaca gttgcaggaa gtgcgggaag
     7081 atgatgacga cgcggagatg acccactcgg tggcagtaaa cgtctacccg gcaacatcgc
     7141 ggatgccaaa attaaccatt gtggtggtgg ataccattcc ggtggagcta aaaagttcct
     7201 atatggtctg gagctggttt atctatgtgc tctcagccaa tctgctgtta gtgatcccgc
     7261 tgctgtgggt cgccgcctgg tggagtttac gccccatcga agccctggca aaagaagtcc
     7321 gcgaactgga agaacataac cgcgaattgc tcaatccagc cacaacgcga gaactgacca
     7381 gtctggtacg aaacctgaac cgattgttaa aaagtgaacg cgaacgttac gacaaatacc
     7441 gtacgacgct caccgacctg acccatagtc tgaaaacgcc actggcggtg ctgcaaagta
     7501 cgctgcgttc tctgcgtagt gaaaagatga gcgtcagtga tgctgagccg gtaatgctgg
     7561 agcaaatcag ccgcatttca cagcaaattg gctactacct gcatcgtgcc agtatgcgcg
     7621 gcgggacatt gctcagccgc gagctgcatc cggtcgcccc actgctggac aatctcacct
     7681 cagcgctgaa caaagtgtat caacgcaaag gggtcaatat ctctctcgat atttcgccag
     7741 agatcagctt tgtcggtgag cagaacgatt ttgtcgaggt gatgggcaac gtgctggata
     7801 atgcctgtaa atattgcctc gagtttgtcg aaatttctgc aaggcaaacc gacgagcatc
     7861 tctatattgt ggtcgaggat gatggccccg gtattccatt aagcaagcga gaggtcattt
     7921 tcgaccgtgg tcaacgggtt gatactttac gccctgggca aggtgtaggg ctggcggtag
     7981 cccgcgaaat caccgagcaa tatgagggta aaatcgtcgc cggagagagc atgctgggcg
     8041 gtgcgcggat ggaggtgatt tttggtcgcc agcattctgc gccgaaagat gaataaatat
     8101 gcccatactt cacgcattac gttaagcatc cgttataatc ggttgcagat accagcctgt
     8161 ggatgcttaa catggaatac caactcactc ttaactggcc cgattttctt gaacgtcact
     8221 ggcagaaacg cccggtggtg ttaaaacgcg gctttaataa ttttattgac ccgatctctc
     8281 cagacgagtt ggcgggtctg gcgatggaaa gcgaagttga cagtcgactg gtcagtcacc
     8341 aggatggcaa atggcaggtc agccacggcc cgttcgaaag ctacgatcat ctcggtgaaa
     8401 ccaactggtc attactggta caggcagtga accactggca tgagccgacc gccgcgctga
     8461 tgcgaccgtt ccgtgaacta ccggactggc gtattgatga tctgatgatt tctttttctg
     8521 tacccggcgg cggcgtcggc ccgcatctcg atcagtacga cgtgtttatc attcagggta
     8581 ccggacgtcg tcgctggcga gtgggcgaaa agctgcaaat gaaacagcac tgcccacatc
     8641 cggatctgtt acaggtcgat ccgttcgaag ccatcatcga tgaagagctg gagcctggtg
     8701 atattcttta tattccgcca ggattcccgc atgaaggcta cgcgctggaa aatgcgatga
     8761 actattccgt gggctttcgc gcgccaaata cgcgggaact gattagtgga tttgccgatt
     8821 atgtgctgca acgtgaactg ggcggcaact actacagcga tcctgatgtt ccacctcgcg
     8881 ctcatcctgc ggatgttctg ccgcaagaga tggataaact gcgtgagatg atgctcgaat
     8941 tgatcaacca gccggaacac tttaagcaat ggtttggcga gtttatatcc cagtcacgtc
     9001 atgaactgga tatcgcgccg ccggaaccgc cttatcagcc agatgaaatc tacgatgcgc
     9061 tgaaacaagg tgatgtgctg gtgcgcctgg gtggtctgcg cgtattgcgc attggcgacg
     9121 acgtgtatgc caatggtgag aagatcgatt ccccgcaccg tccggcacta gatgcactcg
     9181 ccagcaacat tgcgctgact gcggagaatt ttggcgatgc gctggaagat ccgtcattcc
     9241 tcgcgatgct cgcggcgctg gtcaatagcg ggtattggtt cttcgaaggg taagtttgga
     9301 tttaagccgg atgcgacatc acatgccgca tcccttttcg cttacttccg ttgcgccgtt
     9361 aactcggcaa tacggacgat cacctgcacc gctttttcca taccttccag agtcacaaac
     9421 tcatgcttac catgatagtt gtaaccgcca gtgaacaagt tcgggcacgg taatcccata
     9481 aacgacaagt gcgcgccgtc ggtaccaccg cggatcggtt tcagttccgg ttcaatatcg
     9541 cagtcacgca tcgcctgctg ggcgatatcg agaatatgcg gatgctcaac cactttctcg
     9601 cgcatattgt agtaactgtc ttcaatcacc agttcaatgt agcaatcagg atgtaaccct
     9661 ttgcccactt ttttggcgat ctccatcatt ttacgtttac gcgcttcaaa ctgtttacgg
     9721 tcgaaatcac ggatgatgta gtgcatatcg gctcggtcaa cggtgccttt catgctcgcc
     9781 aggtgataga aaccttcata gccttctgtc atttccgggc tttcatccgc cggaacttcc
     9841 gcatgaatac gtgccgccag cgacagcgca tttaccatca ctcctttcgc cgtgcccgga
     9901 tgaacattgt taccgacaat tttgatattg accgatgcgg cgttgaagtt ttcaaactcc
     9961 agttcgccta cgccaccacc gtcaacagtg taagcccagc gggcatcgaa ggcatcaaca
    10021 tcaaaatgtt tcgccccttt gcccacttct tcatccgggg taaaggcgac gcgaatatca
    10081 ccatgcggaa tgtttttctg ttgcaatacc gccagcgcgg tcatgatttc tgcaataccg
    10141 gctttgtcat cggcacctaa caaggtttta ccatcggtag taatcagcgt ctgacccagt
    10201 agctgatgca gcaccgggaa cataaccggt gataaaactt catcgccgat acccagcgca
    10261 atatcgccac cgcgatagtt ttcaacaatt tgcggattca catttttgcc gctgcaatcc
    10321 ggtgaggtat ccacatgaga aataaagcca atcgccggga tatcgccagg gacgttagcc
    10381 ggtaacgtcg ccatcaaagt gcccttctca cttaaggtca cattgataag ccccatctct
    10441 tcgagctgct ctttcagcag atgcaataac ttccattggc cttccgtgct gggaacctgt
    10501 ctcacccctg cttttgattg ggtatccaga gacacgtagt tcaaaaatcg ctcaagtagt
    10561 ttatccatgt agtcaccctc actttttgtg acaacattat taagaagcaa gaaaagacaa
    10621 atattgcgtc aggtcacttt tacccctgca agcgggaata tttatcagca ttaccttaat
    10681 gaatataaag ctaagccagt aattcacaac aaagattggc gattcaagcg gtttgccgta
    10741 gaattaccgc cctcattaag agtcaccaag gtggttaacc acaaaccccg catcggtaag
    10801 ccatccgttg cgtttacatg ggacagagta aaaaattgaa taaacaaccg agttcgcttt
    10861 caccgctggt gcaattggcg ggaattcgca aatgctttga tggtaaagag gtcattcccc
    10921 agctggatct gactatcaac aatggcgagt tcctcacgct gcttggccct tctggctgcg
    10981 gtaaaacaac cgttcttcgc ctgattgcag gtctggaaac tgttgattcc ggacgcatca
    11041 tgctggataa cgaggacatc acccacgttc cggcggaaaa ccgctatgtg aacactgttt
    11101 tccaaagcta cgcacttttc ccccacatga ccgtgttcga aaatgtggcc tttgggttgc
    11161 gcatgcaaaa aacccccgct gctgaaatta cgccccgcgt gatggaagcc ctgcggatgg
    11221 tgcagctgga aaccttcgct caacgcaaac cgcatcagct ctctggtggt caacagcaac
    11281 gcgtcgccat tgcccgcgcg gtggttaaca agcctcgtct gttgttgctg gatgagtcgc
    11341 tctcagcgct ggattacaaa ctgcgcaagc aaatgcagaa cgagctgaaa gcgttacagc
    11401 gtaagcttgg tattaccttc gtctttgtca ctcacgatca ggaagaagcg ctcaccatgt
    11461 cagacaggat tgtggtgatg cgcgagggtc gtattgagca agatggtaca ccgcgcgaaa
    11521 tttacgaaga gccgaaaaac ctgtttgttg ccggcttcat tggcgaaatc aatatgttta
    11581 acgccactgt catcgaacgt ctcgacgagc agcgcgtacg cgccaacgtt gaaggccgcg
    11641 aatgtaatat ctacgttaac ttcgccgttg aaccggggca aaaactgcat gttctgctgc
    11701 gcccggaaga cttacgtgtt gaagagatta acgacgacaa ccacgctgaa gggctgattg
    11761 gttacgttcg tgagcgtaac tacaaaggca tgacgctgga gtcggttgtt gaactggaaa
    11821 atggcaagat ggtgatggtc agcgaattct tcaatgaaga cgaccctgac tttgaccact
    11881 ctctcgacca aaaaatggcc attaattggg tagaaagctg ggaggtcgta ctggctgatg
    11941 aagaacacaa gtaagttcca gaatgtagtg attgtcacta ttgtcggttg gcttgtgttg
    12001 tttgtctttc tgcccaacct gatgatcatt ggcaccagct ttttgacccg cgacgacgcc
    12061 agtttcgtca aaatggtctt tacgctggat aactacacgc gtctgctcga tccgctctat
    12121 tttgaagtgc tattgcactc gctgaatatg gcgctgatcg ccaccctcgc ctgcctggtg
    12181 ctgggctacc cgtttgcctg gtttctggcg aagctgccac acaaggtgcg tccgctgctg
    12241 ctgtttctgc tgattgttcc cttctggacc aactcattga ttcgtatcta cgggctgaaa
    12301 attttcctca gcaccaaagg ctatctcaac gagtttttgc tctggctggg cgttatcgac
    12361 acaccaatcc gtatcatgtt cacgcccagt gcggtgatta tcggtctggt ttacattctg
    12421 ctgccgttta tggtgatgcc gctgtactcc agtatcgaaa aactggataa gccgttgctt
    12481 gaggcggcgc gcgatctcgg tgccagcaag ttacagactt ttatccgcat aattattcca
    12541 ctgacgatgc cggggattat tgccggatgt ctgctggtga tgctgccagc gatgggcctg
    12601 ttctatgtgt ccgacctgat gggcggtgcg aaaaacctgc tgatcggtaa cgtcatcaag
    12661 gtacagttcc tcaatattcg tgactggccg tttggtgcag ccaccagcat tacgctgact
    12721 atcgtaatgg gcctgatgtt gctggtttac tggcgcgctt ctcgtctgct gaataaaaag
    12781 gtggaactcg aatgatcggt cgactgcttc gcggcggttt tatgaccgct atctacgcgt
    12841 acctgtatat cccaatcatt attttgattg tgaactcctt taacagctcg cgctttggca
    12901 tcaactggca gggttttacc accaaatggt atagcctgct gatgaacaac gacagcctgt
    12961 tacaggcagc gcagcattca ctaacaatgg cggtgttttc ggcgacgttt gctacgctta
    13021 tcggttcact gacggcagtt gcgctgtacc gttatcgctt tcgcggtaag ccgttcgtta
    13081 gcggaatgct gtttgtggta atgatgtcac cagatatcgt gatggcaatt tcgctgctgg
    13141 tgctgtttat gctgctgggt attcagcttg gcttctggtc gctgctgttc tcgcatatca
    13201 ccttctgcct gccatttgtg gtggtgacgg tgtattcgcg cctgaaaggt tttgacgtgc
    13261 ggatgctgga agcggcgaaa gatctcggtg ccagcgaatt taccattctg cgaaaaatca
    13321 ttctgccact ggcaatgcca gcggtggcgg cgggctgggt gttaagcttt accctgtcga
    13381 tggacgatgt ggtagtttct tcgttcgtca ccggaccaag ctatgaaatt ctgccgttaa
    13441 aaatttattc gatggtcaaa gtcggcgtat cgccggaagt taacgcgctg gcaaccatat
    13501 tactggtgct gtcgctggtg atggttattg ccagccagct tattgctcgt gataaaacga
    13561 aaggtaacac aggggacgtt aaatgaaaaa atggtcacgc cacctgctcg cggcgggtgc
    13621 tctggcactg ggcatgagcg ccgctcacgc cgatgacaac aacacgctgt atttctacaa
    13681 ctggaccgag tacgtgccgc caggactgct tgaacagttc accaaagaaa ccggtattaa
    13741 ggttatctat tcgacttacg agtcgaacga aaccatgtat gccaagctga aaacgtacaa
    13801 agacggtgcc tatgacctgg tggttccttc aacctattac gtcgataaaa tgcgtaaaga
    13861 agggatgatc cagaagatcg acaagtcgaa gttaacgaac ttcagcaatc tcgatccaga
    13921 catgctcaat aagccgtttg acccgaataa cgattactcc attccgtata tctggggtgc
    13981 gacggcgatt ggtgttaacg gtgatgcggt ggatccgaaa tctgtcacca gctgggccga
    14041 tctgtggaag cctgagtaca aaggcagcct gctgttgacc gacgatgccc gtgaagtgtt
    14101 ccagatggcg ctgcgtaagc tgggctactc cggtaacacc accgatccga aagagattga
    14161 agctgcatat aatgagctga aaaaactgat gccaaacgtc gcagcgttta actccgataa
    14221 cccagctaac ccgtacatgg aaggcgaagt taacctcggc atgatctgga acggttctgc
    14281 tttcgttgca cggcaggcgg gtacgccaat tgacgtggtg tggccgaaag aaggcggcat
    14341 tttctggatg gacagcctgg cgatcccggc aaatgccaaa aacaaagaag gcgcgctgaa
    14401 attgatcaac ttcctgctgc gcccggatgt ggcaaaacag gttgctgaaa ctatcggtta
    14461 tccaacgcca aaccttgcgg cgcgtaagct gttaagtccg gaagtggcga acgataaaac
    14521 gctctacccg gatgctgaaa ccattaaaaa tggcgaatgg cagaatgacg ttggcgcagc
    14581 cagcagcatt tatgaagagt attatcagaa gctgaaagca ggacgttaat tcagcaagcg
    14641 ggcggtggca ttaccgcccg ttttttattt agcaaggatg tggctaatga gtcaggacag
    14701 taaagtttta tcccgctctt tcgtcggtat tgtggtcccc ttgcaggcct gataagcgta
    14761 gtgcatcagg caatgcttcc cgcttttaat cccttcagca acttttcaac aaactcaggc
    14821 accacctggc ttgccgggcc gtaatatttc tcggcaaatt cattaccaac ctgactcggt
    14881 tcaagattca gttccacggt gtgcgcgcca tgcagtttcg cttcgtgaac aaacccagcc
    14941 gccggataaa catgcccgga tgtgccaatg gcaatgaaaa tatcggccat cgacaacgcc
    15001 atataaattt catccatgcc gagtggcatt tcgccaaacc acactacatg cgggcgcaag
    15061 ggtgccggaa actggcagca atggcattta tcttctgggg taacgtctcc ggtccagtcg
    15121 agaacctgac cactttgtga acaacgaact ttcagcagtt ccccatgcat atgaatcaca
    15181 ttggtattac ctgcgcgttc atgcaggttg tcgatattct gcgtcaccag caaaaagcga
    15241 tcgccgaggg cgtcttgcag tttagccagc gcaagatgcg cggcgttagg ctgaatttct
    15301 ggctgctgca gctgtcgacg acgggcgtta taaaacgctt gcaccagttc aggatcgcga
    15361 tcgaaacctt ccggcgttgc cacatcttca acccgatgtt cttcccacag gccatctgcg
    15421 gcgcgaaagg tacgaatacc tgattccgca gaaattcctg cccctgtcag tacgagtact
    15481 cttggttttt ccattgcttc cggcaccact ttatctctga aaaaaatacg ctgacgcaaa
    15541 cgctcgcgca ggcggcgttt atttttacga aaacgactta accgatgacc ccgacgcgac
    15601 agcatagcaa cctctgttgt ttaatcggtt agatgtagga aggccgcacc acgcattcct
    15661 cccgcatcac cgtggcgcgc gcgttcaatg cgcggaacac gagctacagg taagagatga
    15721 cgaggcagcc tgtccgccag ttgcgctgtg attgccggga aattcgataa gccaccacca
    15781 atgacgacca ggtcagggtc aacaatggtc aggatatttc ccagacaaac cgctaataaa
    15841 tccagataac gctcaacgtg cgcccttgcc tgctcatcgc cttgatcata aagcgcaata
    15901 atttcgggag cctgcaacgg ttgatgataa tagtgttgat acagccacgc aaaaccgcga
    15961 ccagacagat aattttcaat gcagccatgc tgaccacagc cgcagcggcg taacgggaaa
    16021 tccaacccca tcatggttaa cgcatcaacc ggcagacgca tatggccaaa ctcgccggta
    16081 atgtagcttt taccggtaat cggtttgccg ttgaaaatca gcccgccgcc aacgccggtg
    16141 ccgagaatca accccatcac cagtggatat tgagtaaatt catcatccca ggcttctgaa
    16201 agggcaaaac agttggcatc gttatcaagg cgtacatcgc gatcaagacg tgcgctcagg
    16261 tcggcacgca gcggtttacc gctggcagcg gggacattgg cggcatacag cgtaccatct
    16321 tctgtttccg gcattcccgg aataccgatg ccgacagaac ctttacagcc aaaacgttgg
    16381 tcagcttcag ctaccagctc acacactgca tctaaaaatg catcatagct gtcacgcggt
    16441 gtcggcaccc gcttttccca ctgcaactgc cgaccgctat caaacacgcc aagcgcaatt
    16501 tttgttccac caatatcaaa cccgtaatac attgccgctc cttgttttaa tgtactgcct
    16561 ttactggccg ctaaggactc gcgcagggtc aatattactg gcgcgccgcg ccggatacca
    16621 acttgccaaa agactcagca acaatgctgt gaccagtacg tagaagacgt ccagccagtg
    16681 caattccgat ggcaggaaat caataaaata gatatcgctg gagaggaact gatgaccgat
    16741 caacttttca atccactcaa taatcggggt aagttgcagt gaaaccacta cgccgataat
    16801 cacaccacac aggctgccga acagccctgc cagcaatcca taccagacaa agatggcgcg
    16861 aattaaacca tctttcgccc ccagcgttct taatactgcg atatcgccac tcttgtcttt
    16921 caccgccatc actaaggtgg agacgatgtt gaaacaggcc acgccaatca ccagtaccat
    16981 cgccagatac ataatggcgc ggatcatttg gatatcgcga tacatatagc cgtaagtacc
    17041 aatccagctt ttaatataaa catagctgtt ggtcacttca cccgcatcgc gtaccagctt
    17101 attggcgttg aaaacatccg tcattttaag ggcaatacct gacacgctgg aacccatatc
    17161 aagatattgt tgggcatctg ccagcgggat catggcaaaa ctgtgatcga gttgaccact
    17221 caactgcaaa ataccggcaa cgtgcaaacg cacacgtttt ggctgcatca gtttatgctc
    17281 gggattcgag ttggggatca taatcgacac ccaatccccc tgcttcactt tcagcgcatc
    17341 cgccacgcct ttgccgatga taatttgctg ttcgcccgct ttaaaattgc gccacgcatc
    17401 gccctggaca aacgagggta atgcgctcag acgctgttcc tgttgcgggt taacgccctt
    17461 cacctggatt gcgcgaagat tcgcgccact ttccatcagc ccggtgaaat tgatatacgg
    17521 cgcggcagcg gcaatacccg gcactttttg cacgtgatcc agtgcttcct gccagttagt
    17581 ccacggctga tccaccgcct cgatttcacc atgcggcacc accgccagaa tacggttgtt
    17641 cagttcgcgt tcaaagccgt tcatcgcgct taagccgacg atcaataccg ccacgccgag
    17701 ggcaatgcca atggtagaaa tgacggagat cagcgacacc atgccgccgc gccgccgtcc
    17761 gcgactaaaa cgcaggccaa ttaataacga taaaggcatc gccattactc cgcccccatc
    17821 aggctcagtt ccgccgtcag acgaccatca cgcatttcta actggcggct catacgtttc
    17881 gccagttgca ggtcgtgagt aaccaccagg aaggcggtgc cctgcaagcg attcaattcc
    17941 ccaagcaact ggaagatgct gtcggcgtta cgcgcatcga ggttaccggt aggttcatcc
    18001 gccagtacca ggcgcgggtt attcaccagc gcacgggcaa tcgccacacg ctggcgttcg
    18061 ccgccagaaa gttcagatgg gcggtgattc gcacgatgat ccagccccac cgcttttaac
    18121 atctcaagtg cacggctgtt gatttcagcg ggctttttct tgccaatcag cagcggcata
    18181 gccacgtttt ccagggcggt aaaatccggc agcaggtggt gaaactgata aataaagccc
    18241 agcttctggt tgcgcagttc agctttcgct gccgacgaca gtttgctcat tggctgacca
    18301 ttaaaaatca catcgccgga ggttggcgta tccagcccgc ccagcaggtg cagcaaggta
    18361 cttttaccgg aaccagagct accgacgatc gccatcattt caccttcgcc gacgctgaaa
    18421 ctgacattgt gcagcacatc ggtttgcaca ctgccttcct gatagcgttt gcacaggttg
    18481 tcgcattgca acaggatctt attcataacg taaagcctca gcgggttgag tggcggcagc
    18541 gcgccatgaa gggtaaagcg tagacagcag cgcgatagcc atcgccacca gcgcaataac
    18601 aatgacctgt aaaggttcga tagccaccgg cagcgccgcg ccatcaagca ggacgccgat
    18661 tatcggcatc agattattta actggctggc aagcagggcg ccaagcgccg ctccgaggat
    18721 cgcaccgata atcccggcgc tggccccttg caccataaag accatcatga tttgtcgcgg
    18781 agttaagcct tgcgtttgca ggatcgctac ttcgccctgc ttctccatta ccatcagccc
    18841 tagtgaggta ataatgttaa acgccgcaac ggcgacaatc aggctcagca gtaaacccat
    18901 catatttttt tccatgcgta cggcctggaa caactcgcct ttacgatcac gccagtcctg
    18961 ccatttgctg ccttcaggca gtttttgctg acttaatgag tcgactttca gcggctcatc
    19021 cagccacaaa cgccagccgg taatattgcc tgccggataa cgcatcagac gcgaggcatc
    19081 ctcaatattc accagcattt cattgccatc gacttcactg ttggcggcga aagtgccaat
    19141 cacattgaac aggcgctgac ttggaatacg ccccatcggc gtgaactggc tggcagatgg
    19201 taccatcacg cggatttgat caccgcgatt aacgcctagc tgtgaggcaa gttgttcgcc
    19261 gaggatgaca ttatatttcc ccggctcgag gtcagtttgt ttcacattga ccagatacgg
    19321 tgtaagtgga tctttttgcg ccgggtcgat accgagcatc accccgaccg ccacgctgcg
    19381 cgcgctttgc agtaccacat caccggtagt aataggtgcg acgcgattaa cgccgtccag
    19441 tttgactgcc gtttccggga gttgctgccg gttaagagag ccatgctcag aagagagaat
    19501 tgcctgtggc atcaggccaa ggatgttgtt ttgcagctcg cgctcaaagc cgttcatcac
    19561 tgacaatact gtgaccagcg ccatcacccc gagggtaatg ccgatggtag aaagccagga
    19621 gacgaaacga ctgaagcgat ccgctgcacg cccacgcatg taacgcaggc caataaatag
    19681 agcgacaggt tggtacatga aatccgtctg gttgctgtag caaagacacg agtatataag
    19741 cgaaagcaag atgggtaaac gtctgaactg taagtgatcg tcagggctaa tctgaaaagc
    19801 ccccgtaaat caatcggcta aattgccatg ttttatcatc aatcttgccg cctgatctta
    19861 atctgaaaaa tcatccaata cagacagata cccattacac atagggtcat aacccgcagg
    19921 atcaaccaca tgtaaattaa aaaagagcgt gttgtcctga tgaaacaaaa agagctatgg
    19981 attaaccaga tcaaagggtt atgtatttgt ctggtggtga tttatcactc ggtcattacc
    20041 ttttatccgc atctgaccac ttttcagcat ccgttatcgg aagtcctgag caaatgctgg
    20101 atctatttca atctttacct tgcacccttt cgtatgccgg tttttttctt tatttccggc
    20161 tatttgattc gccgctatat cgacagcgta ccgtggggaa attgtctcga taaacgcatc
    20221 tggagcatct tttgggtgct ggcgctttgg ggcgtggtgc agtggctggc gttaagtgcg
    20281 ctgaaccagt ggctggcacc tgagcgcgat ttaagcaatg cctccaatgc cgcttatgcc
    20341 gattccaccg gtgagttcct gcacgggatg atcaccgcca gcaccagctt gtggtatctg
    20401 tatgcgttaa ttgtctattt cgtggtatgt aaaattttta gccgtctggc gctgctacta
    20461 ttcgccttgt ttgtactgct gagtgtggcg gttaatttcg ttcccacgcc gtggtgggga
    20521 atgaacagtg tgatccgcaa tttgccttat tacagccttg gcgcatggtt tggcgcaaca
    20581 ataatgacct gtgttaaaga ggtgccgttg cgccgccatc tgctaatggc ttctttgctt
    20641 accgttctgg cggtcggtgc ctggttgttt actatctcgc tgctgttgtc gctggtatcg
    20701 attgtggtga tcatgaagct gttttatcaa tacgagcaac gattcggtat gcgctccacc
    20761 agcctgctga atgtgattgg ctccaacacc attgctatct acaccaccca tcgcattctg
    20821 gttgaaatat tcagcttaac tctgcttgcg caaatgaacg cagcacgctg gtcgccgcaa
    20881 gtcgaactga cactcctgct ggtttatccc tttgtcagtt tgttcatctg tactgttgcg
    20941 ggcttgctgg taagaaaact ttcacagcgt gcattcagcg atctgttgtt ctccccgcct
    21001 tcactgcccg ctgccgtcag ttactcccgc taagccatcg ccccagccgg ggcgattcaa
    21061 tttgctaaac catgtcgatt acggataatg aacagcattg cttatcagag atatgccccc
    21121 atatgttgag gcatatccta acgagaatct gacaaccgtt atgcctgaac aatatcgtta
    21181 tacgctgccc gtcaaagcgg gtgagcagcg tctgctgggc gagttaaccg gcgcagcctg
    21241 tgcaacgctg gtagcggaaa ttgccgaacg tcacgccggt ccggtggtac tcattgcacc
    21301 agatatgcaa aatgctctgc gtttgcatga tgaaatcagc cagttcaccg atcagatggt
    21361 gatgaatctg gcggactggg aaactcttcc ctacgacagt ttttcgcctc atcaggacat
    21421 tatctcctcg cgcctttcca ccctttacca gctaccgacg atgcagcgtg gcgtactgat
    21481 tgttccggtg aatacgctta tgcagcgcgt ttgcccacac agttttctcc acggtcatgc
    21541 gctggtgatg aaaaaaggtc agcgcctgtc acgagatgca ttacgaaccc aactggacag
    21601 cgccggttat cgccatgttg accaggtgat ggagcacggc gaatacgcca cgcgcggcgc
    21661 gttgctggat ctcttcccga tggggagtga gctgccttat cgtcttgatt tctttgatga
    21721 tgaaatcgac agcctgcggg tgtttgacgt cgacagccag cgcacgctgg aggaagtaga
    21781 agcgatcaat ctgctgcccg cgcacgaatt tccgaccgat aaagcggcaa ttgaactgtt
    21841 ccgcagccag tggcgcgata ccttcgaagt gaagcgcgat ccggaacata tttaccagca
    21901 agtgagtaaa ggcacattac ctgccgggat cgagtactgg cagccattgt tcttcagcga
    21961 accactgccg ccgctgttca gttatttccc tgccaatacc ttgctggtga atactggcga
    22021 tctggaaacc agtgccgaac gtttccaggc tgacacgctg gcgcgttttg agaatcgcgg
    22081 cgtcgatccg atgcgcccgc tgttgccacc acaatcgctc tggctgcggg tggacgagct
    22141 cttctcagag ctgaaaaact ggccccgggt gcagctaaaa actgaacatt taccgacaaa
    22201 agccgcgaat gccaatttag gtttccagaa actgccagac ctggccgttc aggcgcaaca
    22261 aaaagcgccg ctggatgcgc tgcgtaagtt cctcgagact ttcgacggtc cggtggtgtt
    22321 ctcggtagaa agtgaaggtc gccgtgaagc gctgggtgaa ctgctcgcac gaattaaaat
    22381 tgctccgcaa cgcattatgc gtcttgatga agccagcgac cgtgggcgtt atctgatgat
    22441 tggcgctgcc gaacatggtt ttgtcgatac ggtgcgtaat ctggcgctga tctgcgaaag
    22501 cgatctgctc ggtgaacgtg ttgcccgccg tcgtcaggat tctcgccgca ccatcaaccc
    22561 cgatacactg atccgtaacc ttgcggagct gcatattggt cagccggtgg tccatctgga
    22621 gcacggtgtc gggcgctatg ccggaatgac cacgctggaa gcgggcggca ttaccggcga
    22681 gtacctgatg ctcacctatg ccaacgacgc caaactgtat gttccggtgt cgtcactgca
    22741 tctgattagc cgttacgcag gtggcgcgga agaaaacgcc ccgctgcata aacttggcgg
    22801 cgatgcgtgg tcacgcgcgc ggcagaaagc ggcggaaaaa gtgcgtgatg tggcggcgga
    22861 attgctggat atctacgcgc aacgcgccgc caaagagggc ttcgcgttta aacacgatcg
    22921 tgagcagtat cagttgttct gcgacagctt cccgtttgaa accacgccgg atcaggcgca
    22981 ggccattaat gcggtactta gcgacatgtg tcagccgctg gcaatggatc gtctggtgtg
    23041 cggcgatgtt ggctttggta aaacagaagt ggcgatgcgc gccgctttcc tggcagtaga
    23101 taaccacaag caggtagcgg tgttggtgcc taccaccctt cttgcgcagc agcattacga
    23161 caacttccgc gaccgtttcg ccaactggcc ggtacgtatc gaaatgatct cccgtttccg
    23221 cagcgccaaa gagcagacgc aaatccttgc ggaagtggcg gaagggaaaa tcgatattct
    23281 gatcggtacg cacaaactgc tgcaaagtga cgtcaagttt aaagatttag gcctgctgat
    23341 tgtcgatgaa gaacaccgct tcggggtgcg tcataaagag cgcattaaag cgatgcgcgc
    23401 gaacgtggat attctgacgc ttactgcaac gccgatccca cgcacgctga atatggcaat
    23461 gagcggaatg cgtgacctgt cgattatcgc cacgccgccc gcccgtcgtc tggcagttaa
    23521 aacctttgtc cgtgagtatg acagcctggt ggtccgggag gcgatcctgc gtgaaatttt
    23581 gcgcgggggg caggtttatt atctctacaa tgatgtggaa aacatccaga aagctgccga
    23641 acggctggca gaactggtgc ctgaagcacg gattgccatc ggtcacgggc agatgcgcga
    23701 gcgcgaactg gaacgggtga tgaatgattt ccatcatcaa cgtttcaacg tgctggtttg
    23761 taccaccatt atcgaaaccg ggatcgacat cccgacagcc aacaccatta tcattgaacg
    23821 tgcggatcac ttcggtctgg cgcagctgca ccagttacgc ggtcgcgtcg gacgttcaca
    23881 tcatcaggca tatgcatggc tgctgacgcc gcatccaaaa gcgatgacta ccgatgcaca
    23941 aaaacgtctt gaagcgattg cctcgctgga agatctcggt gcaggctttg cgctggcaac
    24001 gcacgatctg gagatccgcg gcgcgggtga actgcttggc gaagaacaaa gtggctcaat
    24061 ggaaaccatc ggtttctcgc tgtatatgga gttgctggaa aacgccgtcg atgcactgaa
    24121 agccggacgc gagccgtcgc tggaagatct caccagccag caaacagaag tcgagctgcg
    24181 gatgccgtcg ctattgccag atgatttcat tcctgacgtg aatacgcgtt tgtcgttcta
    24241 taaacgtatt gccagcgcca aaacggaaaa cgaactggaa gagatcaaag tcgagcttat
    24301 cgatcgcttc ggcctgctgc cggatccggc gcgtaccctg ctggatgttg cccgtctgcg
    24361 ccagcaagcg cagaaactgg ggatcaggaa gctggaaggt aatgagaaag gcggggtgat
    24421 cgaatttgcc gagaagaatc acgttaatcc ggcctggttg attggtttgc tgcaaaaaca
    24481 gccgcagcat taccgccttg atggtccgac gcgcctgaaa tttattcagg atttgagtga
    24541 gcggaaaacg cgtatcgaat gggtacgcca gtttatgcgt gaactggaag agaacgcgat
    24601 cgcttaatgg taaatcgggg gcgtttctgc gcccccatct gcaacattta caaattcttt
    24661 gcacttccct gcactatccg acactgtcac catccataat tcaggcttat ctgtttatta
    24721 caataacctt atatttatta tggatttttg gtgatgatca aaacgcgttt ttctcgctgg
    24781 ctaacgtttt ttacgttcgc cgctgccgtg gcgctggcgc taccggcaaa agccaacacc
    24841 tggccgctgc cgccagcggg cagtcgtctg gttggcgaaa acaaatttca tgtggtggaa
    24901 aatgacggtg gttctctgga agccatcgcc aaaaaatata acgtcggctt tctcgctctg
    24961 ttacaggcta accctggcgt tgatccttac gtaccgcgcg cgggtagcgt gttaacgatc
    25021 ccgttgcaaa ccctacttcc agatgcgccg cgcgaaggca ttgtgatcaa tattgcggag
    25081 ctgcgtctct attactaccc gccgggtaaa aattcggtaa ccgtgtatcc aataggtatt
    25141 ggtcagttag gtggtgacac gctgacaccg acaatggtga ccaccgtttc agacaaacgt
    25201 gcaaacccaa cctggacgcc aacggcaaac atccgcgccc gttataaagc acagggaatt
    25261 gagttgcctg cggtagtgcc ggctggaccg gataacccaa tgggccatca tgcgattcgt
    25321 ctggcggcct atggcggcgt ttatttgctt cacggtacga acgccgattt cggcattggc
    25381 atgcgggtaa gttctggctg tattcgtctg cgggatgacg atatcaaaac actctttagc
    25441 caggtcaccc caggcaccaa agtgaatatc atcaacactc cgataaaagt ctctgccgaa
    25501 ccaaacggtg cacgtctggt tgaagtacat cagccgctgt cagagaagat tgatgacgat
    25561 ccgcagctgc tgccaattac gctgaatagc gcaatgcaat catttaaaga tgcagcacaa
    25621 actgacgctg aagtgatgca acatgtgatg gatgtccgtt ccgggatgcc ggtggatgtc
    25681 cgccgtcatc aagtgagccc acaaactctg taaatcatgg ataaaaaaaa tccccgcgac
    25741 acggcatgtc gcggggaaaa aatcagcagt agcaggcatt aatgagggtt aatgcttatt
    25801 tataaattac tgctgttcca tggagggtat tcggaccggt tacagaagta atacggaaag
    25861 attttgcgcc catctcatcc gctttttgcg ccatctgctc ttccagcgat cccagatttg
    25921 tccccgcgtt agcactgatt gtaccgactt tttgttggcc ttctggcgtt gactgaactt
    25981 cgacagccgc aaagctggca aatgacatgg agcttaaaat cgccgcagcg atgagggttt
    26041 ttacattttt cataatagtg gccttatgca gatgaatgac gtcgcaagca attacttaac
    26101 gatcgataac taaatga
//
LOCUS       DAPHBJ010000049        26076 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.049, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000049 DAPHBJ010000000
VERSION     DAPHBJ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.049"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..482)
                     /locus_tag="RHZ76_001809"
     CDS             complement(<1..482)
                     /locus_tag="RHZ76_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDV2812461.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     "
     gene            complement(553..2667)
                     /gene="fusA"
                     /locus_tag="RHZ76_001810"
     CDS             complement(553..2667)
                     /gene="fusA"
                     /locus_tag="RHZ76_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDV2812462.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2764..3234)
                     /gene="rpsG"
                     /locus_tag="RHZ76_001811"
     CDS             complement(2764..3234)
                     /gene="rpsG"
                     /locus_tag="RHZ76_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDV2812463.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3331..3705)
                     /gene="rpsL"
                     /locus_tag="RHZ76_001812"
     CDS             complement(3331..3705)
                     /gene="rpsL"
                     /locus_tag="RHZ76_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDV2812464.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3831..4118)
                     /gene="tusB"
                     /locus_tag="RHZ76_001813"
     CDS             complement(3831..4118)
                     /gene="tusB"
                     /locus_tag="RHZ76_001813"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HDV2812465.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4126..4485)
                     /gene="tusC"
                     /locus_tag="RHZ76_001814"
     CDS             complement(4126..4485)
                     /gene="tusC"
                     /locus_tag="RHZ76_001814"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HDV2812466.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4485..4871)
                     /gene="tusD"
                     /locus_tag="RHZ76_001815"
     CDS             complement(4485..4871)
                     /gene="tusD"
                     /locus_tag="RHZ76_001815"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HDV2812467.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4871..5593)
                     /locus_tag="RHZ76_001816"
     CDS             complement(4871..5593)
                     /locus_tag="RHZ76_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV2812468.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5760..6572)
                     /gene="fkpA"
                     /locus_tag="RHZ76_001817"
     CDS             complement(5760..6572)
                     /gene="fkpA"
                     /locus_tag="RHZ76_001817"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDV2812469.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6793..7011
                     /locus_tag="RHZ76_001818"
     CDS             6793..7011
                     /locus_tag="RHZ76_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HDV2812470.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7060..7650)
                     /gene="slyD"
                     /locus_tag="RHZ76_001819"
     CDS             complement(7060..7650)
                     /gene="slyD"
                     /locus_tag="RHZ76_001819"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDV2812471.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7745..7945)
                     /locus_tag="RHZ76_001820"
     CDS             complement(7745..7945)
                     /locus_tag="RHZ76_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HDV2812472.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7955..9760)
                     /gene="kefB"
                     /locus_tag="RHZ76_001821"
     CDS             complement(7955..9760)
                     /gene="kefB"
                     /locus_tag="RHZ76_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HDV2812473.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9760..10311)
                     /gene="kefG"
                     /locus_tag="RHZ76_001822"
     CDS             complement(9760..10311)
                     /gene="kefG"
                     /locus_tag="RHZ76_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HDV2812474.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10442..12355
                     /locus_tag="RHZ76_001823"
     CDS             10442..12355
                     /locus_tag="RHZ76_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2812475.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQADEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12355..13377
                     /locus_tag="RHZ76_001824"
     CDS             12355..13377
                     /locus_tag="RHZ76_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDV2812476.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13371..13589
                     /locus_tag="RHZ76_001825"
     CDS             13371..13589
                     /locus_tag="RHZ76_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HDV2812477.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13643..14512
                     /locus_tag="RHZ76_001826"
     CDS             13643..14512
                     /locus_tag="RHZ76_001826"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HDV2812478.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14567..14971)
                     /locus_tag="RHZ76_001827"
     CDS             complement(14567..14971)
                     /locus_tag="RHZ76_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HDV2812479.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVGA"
     gene            15273..15905
                     /gene="crp"
                     /locus_tag="RHZ76_001828"
     CDS             15273..15905
                     /gene="crp"
                     /locus_tag="RHZ76_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HDV2812480.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15956..18046
                     /locus_tag="RHZ76_001829"
     CDS             15956..18046
                     /locus_tag="RHZ76_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDV2812481.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18113..19333)
                     /gene="argD/dapC"
                     /locus_tag="RHZ76_001830"
     CDS             complement(18113..19333)
                     /gene="argD/dapC"
                     /locus_tag="RHZ76_001830"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HDV2812482.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGHKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGQARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19419..19982)
                     /gene="pabA"
                     /locus_tag="RHZ76_001831"
     CDS             complement(19419..19982)
                     /gene="pabA"
                     /locus_tag="RHZ76_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HDV2812483.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20014..20616)
                     /locus_tag="RHZ76_001832"
     CDS             complement(20014..20616)
                     /locus_tag="RHZ76_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HDV2812484.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20606..20773)
                     /locus_tag="RHZ76_001833"
     CDS             complement(20606..20773)
                     /locus_tag="RHZ76_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HDV2812485.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20878..21450)
                     /gene="ppiA"
                     /locus_tag="RHZ76_001834"
                     /gene_synonym="rotA"
     CDS             complement(20878..21450)
                     /gene="ppiA"
                     /locus_tag="RHZ76_001834"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HDV2812486.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21721..22902
                     /gene="tsgA"
                     /locus_tag="RHZ76_001835"
     CDS             21721..22902
                     /gene="tsgA"
                     /locus_tag="RHZ76_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HDV2812487.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23165..25708
                     /gene="nirB"
                     /locus_tag="RHZ76_001836"
     CDS             23165..25708
                     /gene="nirB"
                     /locus_tag="RHZ76_001836"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HDV2812488.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILSSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25705..26031
                     /gene="nirD"
                     /locus_tag="RHZ76_001837"
     CDS             25705..26031
                     /gene="nirD"
                     /locus_tag="RHZ76_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HDV2812489.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
BASE COUNT         6348 a         7055 c         6539 g         6134 t
ORIGIN      
        1 tactgagaca gaagttcacg aacttccatt tcaaccagtt ccagcagctc ttcgtcatca
       61 accatatcgc atttgttcag gaacacgatg atgtacggaa cgcctacctg acgacccagc
      121 aggatgtgct cacgagtctg cggcatcggg ccgtcagtcg cagcaactac caggatcgcg
      181 ccgtccatct gagcagcacc ggtgatcatg tttttaacat agtcggcgtg ccccgggcag
      241 tctacgtgtg cgtagtgacg ggtcggggtg tcgtattcaa cgtgagaagt gttgatggtg
      301 ataccacgag ctttttcttc cggcgcgtta tcgatctggt cgaatgcacg agcagcaccg
      361 ccgtaggttt tagccagtac ggtggtgatt gcagcggtca gagtagtttt accgtggtca
      421 acgtggccga tagtaccaac gttaacgtgc ggttttgtac gttcaaattt ttctttagac
      481 acggctatat tccttactat agtgctctcc ccttcaggag agagcacggg actttggtat
      541 taacccttag gcttatttac cacgggcttc aattacggcc tgagcaacgt tactcggcgc
      601 ttcatcatac ttcaggaatt ccatagtgta tgatgcacga cctttggtca gagaacgcag
      661 ctgagttgcg tatccgaaca tttcagacag cggtacttca gcgtggatct taacgccagt
      721 aacttcagat tcctgacctt tgagcatacc acgacgacgg ctcaagtcac cgataacgtc
      781 accggtgttc tcttccggag tttctacttc aaccttcatg atcggctcaa gcagaactgg
      841 tttcgctttc ttaaagcctt ctttaaaggc gatagaagca gccagtttaa acgccagttc
      901 agaggagtca acgtcatggt aagaaccgaa gtgcagacga atacccatgt ctactaccgg
      961 gtagcctgcc agcggacctg ctttcagctg ttcctggata cctttatcaa cggccgggat
     1021 gtattcgcca gggattacac cacctttaat gtcgttgatg aactcgtagc ctttcgggtt
     1081 tgaacccggc tccagcgggt acatgtcgat aacaacatga ccatactgac cacgaccacc
     1141 agactgtttc gcgtgtttac cttcaacatc ggtaactttc tggcggatag tttcacggta
     1201 agcaacctgc ggtttaccta cgttcgcttc aacgttgaat tcacgcttca tacggtcaac
     1261 gatgatgtcg aggtgcagtt cgcccatacc cgcaatgatg gtctggttag attcttcgtc
     1321 agtccataca cggaaagacg ggtcttcttt agccagacgg cccagagcca gacccatttt
     1381 ttcctggtca gctttggttt tcggttcaac tgcgatggag attaccggct cagggaattc
     1441 catacgttcc agaatgatcg gcgcatccgg gtcacacagg gtgtcaccag tggttacgtc
     1501 tttcagaccg atagcagcag cgatgtcgcc cgcgcgaact tctttgatct cttcacgttt
     1561 gttagcgtgc atctgaacga tacgaccgaa acgctcacgt gcagctttca cggagttcag
     1621 tacggtatca ccagagttaa ccacaccgga gtaaacacgg aagaaggtca ggttaccaac
     1681 aaacgggtcg gtagcgattt tgaacgccag tgcagagaac ggctcgtcat cacttgcgtg
     1741 acgttcagcc ggagtgtctt taccgtcgtc caggataccg ttgatcgcag gtacgtcaac
     1801 cggggatggc aggtaatcga ttaccgcatc cagcatcgcc tgaacacctt tgttcttgaa
     1861 cgcagaacca caggttacca ggatgatttc gttgttcaga acgcgctgac gcagagcacc
     1921 tttgatttct gcttcagtca gttcttcacc acccaggtat ttttccatca gctcttcaga
     1981 agcttcagct gcggattcga tcaggttctg gtgccattcg ttagccagtt caaccatgtc
     2041 tgccgggata tcttcgtatt cgaaggttac gccctggtca gcgtcgttcc agttgatagc
     2101 tttcattttc accaggtcaa caacaccggt gaaatgttct tcagcaccaa tcgccagctg
     2161 cagcggaacc gggttcgcgc ccagacgggt tttgatctgg ttaacaactt tcaggaagtt
     2221 cgcacccatg cggtccattt tgttaacgaa cgcaatgcgc ggaactttat atttgtttgc
     2281 ctgacgccat acggtttcag actgcggctg aacaccacca actgcgcagt aaaccattac
     2341 cgcaccatcg agaacacgca tggaacgttc tacttcgatt gtgaagtcaa cgtgccccgg
     2401 ggtgtcgatg atgttgatgc gatgcggctc atactgctta gccataccag accagaatgc
     2461 agtagtcgca gcggaagtga tggtaatacc acgttcctgc tcctgctcca tccagtccat
     2521 ggttgcagcg ccgtcatgaa cttcaccgat tttatggttt acaccggtgt agaacagaat
     2581 acgttcggta gtagtggttt taccggcgtc gatgtgcgca ctgataccga tgttacggta
     2641 gcgtgcgatg ggtgttgtac gagccatttg tttcctcgtt tatcttttag gcgttcaatt
     2701 taagtagccc aaagcgggct gcttactgga agcgcccgcc tggtgactaa aactccgaag
     2761 ggattaccaa cggtagtgtg cgaacgcctt gttggcttcg gccatacggt gaacgtcttc
     2821 acgtttctta actgcagtac ctttgttttc tgcagcatca gaaagttcgt tcgccaggcg
     2881 cagagccatg gatttatcac cgcgtttacg agcagcttca acgatccaac gcattgccag
     2941 agcattacga cgaaccggac ggacttcaac tggtacctga taagtagaac caccaacgcg
     3001 gcgagactta acttctacag tcgggcgcac gttttcgaga gctacttcga atgcttccag
     3061 ttcagattta ccagagcgct gagccagggt ctccagcgcg ctgtatacga tagattcagc
     3121 agtagatttt ttaccatcta ccatcaggat atttacaaat ttagccagca gttctgatcc
     3181 gaacttcgga tccggcagaa ttttacgctg accaatgacg cgacgacgtg gcatggaaat
     3241 actccgttgt taattcagga ttgtccaaaa ctctacgagt ttagtttgac atttaagtta
     3301 aaacgtttgg ccttacttaa cggagaacca ttaagcctta ggacgcttca cgccatactt
     3361 ggaacgagcc tgcttacggt ctttaacgcc ggagcagtca agcgcaccac gtacggtgtg
     3421 gtaacgaaca cccgggaggt ctttaacacg accgccacgg atcaggatca cggagtgctc
     3481 ctgcaggttg tgaccttcac caccgatgta ggaagtcact tcgaaaccgt tagtcagacg
     3541 aacacggcat actttacgca gcgcggagtt cggtttttta ggagtggtag tatatacacg
     3601 agtacatacg ccacgttttt gcgggcatgc ttccagcgca ggcacgttgc ttttcgcaac
     3661 tttgcgagca cgtggtttgc gtaccagctg gttaactgtt gccattaaat agctcctggt
     3721 tttagctttt gcttcgtaaa cacgtaataa aacgtcctca cacaatatga ggacgccgaa
     3781 ttttagggcg atgccgaaaa ggtgtcaaga aatatacaac gatcccgcca tcaccaggcc
     3841 atctggctgg agtgcttaac cgtaagtctg acgaaatcag tatagtcaat gggaatgatg
     3901 tcgttcgaaa tttgaccagt caaaccgcgg gcaataaggt cttcgttcag ggcatagacc
     3961 ttaatggggg cattacgcag actttcaagg tagcggttac cgtcaactgc ggcagttacg
     4021 ccatcttgca ataatagcag ttcgtctcct tcactgagca gacgcagcag cgcagcaaaa
     4081 tccgtcagcc agggtgaacg atgtaacgtg tgcagcataa acgcctcaaa acctcaaaat
     4141 aacatcgtag ttggcgagtt cgcggcgtaa ggcatctgct tcgagcggcg tggcttcgac
     4201 aacaaagggt gtctgcggat ctaacccgcg ttcgcgcagt gaagccgcac aaacccagca
     4261 ctgttcaatg tcgtacagac ccaacaattt aaaagtggca atgtaatcac gcgccagcac
     4321 tgcatcgggc ttttgtcctg acagcagctg aaaaacgcca tcagcaataa agaagacggc
     4381 cagatcgtca gttaatgcgg aagttgccag taaagcatct aaaccttccc gaccagcagc
     4441 tgtaccatga ggtgcagtag aaaaaacaaa cgcaattcgt ttcatcagaa ctgtaccacc
     4501 ctgtcacagg tcagcgaggc ttccgccagc gcgccaagtc cgcttaaggt aaatcccggc
     4561 tgaaggtttg acgaagccag ccccagcctt cctgcttcag tttcatcgac gataccgcgg
     4621 cgtaacgctg ccgctacgca gatattcagc gccacaccat gttgcgcatt caactgttgc
     4681 cagccgcgca caaggtcaaa ttcgtcactt gccggtgagg tcaattggtt agcgttatag
     4741 accccttccc gatagaaaaa gacgctgctt aactcatgac cttctactat cagcgcctgc
     4801 gcaaactgaa aagcactact cgcctgttgc gtaccgtatg ctggcccggt caccacgatg
     4861 gcaaaacgca ttacttatct tgcccctgga aatcaccgct tttgaactgg cggatgtaga
     4921 gatagacagt gtgtttggag atgttcaggc ggtcagcaac ctggttgatc gcgtctttaa
     4981 tatcgaagat ccctttctcg tagagattca gcacaatctg acgatttttg gcgttattag
     5041 aaacattgcg atcggcattc acttcttcga tggtgaactc cagcgtttgg gtaaccagat
     5101 cttcaacaga agaggcaaag ttgacgcttg aaccgacatc cggggtttct ggcggcacaa
     5161 aagtgctcat aatctgcgag aagggaacat caagattcat attgatgcac agcagaccaa
     5221 ttacacgctg ttcgcggtta cgaatcgcga tagtcaggga cttcattaat acgccgcttt
     5281 tggcgcgagt aaagtagcat ttagaaacgc tgctatccgc tcccgtcata tcgtgcagca
     5341 tacgtagcgc caggtcagta attggcgaac caatcttccg gccggtatgt tcaccgttag
     5401 caatgcgaat ggctgaacat tttagatcct gcaaagagtg caaaacgatt tcacagtggg
     5461 agccaataag catcgctaac ccgtccacca ccgcttcgta ggatttcaga atatcaaaat
     5521 cggtctggtc gaaaggacgt tgatccagta aatccaactc actggtttcg ttggttaaaa
     5581 gcgacctgga catgaaaaaa aacactcctt ttcaggagcc tgtcgttaac ttttcagggc
     5641 aggctcatta atgatgcggg taactaaatt aatacagcgg aggttccgct ttccagcact
     5701 aattatatcc ggcctgtaat aaaaaaaccg ccgcctggtc aggcggcgat tcttaatgct
     5761 tattttttag cagaatctgc ggctttcgca tcagcttccg gctttgcatc agccttcggc
     5821 gctggtttca catccagcag ctctacgtca aacaccaggg tagaattcgg tgggatcccc
     5881 ggaacacccg ctttgccgta agccagttct ggtggaataa ccagtttgat cttaccgcct
     5941 ttcttgatgt tcttcagacc ttctgtccaa cccgggataa caccgtccag acggaaagaa
     6001 agcggttcac cacgggtgta agagttgtcg aactctttac cgtcgatcag cgtacctttg
     6061 tagttcacta caacagtatc gctgtctttc ggtgcttcgc ctttaccggc ttctactacc
     6121 tgataaacca gaccagttga agaggttttc acacctttct ctttggcaaa tttctcgcgg
     6181 tactctttac cttttgcttc gttatcagcc gcgtcttttt ccatcttcgc ctgagcagaa
     6241 gacttcacgc gagcttcgaa tgcttgcaga gtctgttcga tctcttggtc ggagagtttg
     6301 ctcttatcag caaatgcatc ctgaacacca gcgatcagct gatctttatc cagtttgatg
     6361 cccagttttt cttgttcttt tagagagttt tccatgtaac gacccagcga ggcacccagt
     6421 gcataagctg atttctgatc gtcatttttg aacgctgctt tgctgtcagc agttgtagca
     6481 ggttttgcag cttcagcagc aaaagtgatt ggtgcatgca gggcaacggc cattgtggtc
     6541 gccagcagcg ttactttaaa cagtgatttc atccatatct ccaggatcgg ggcatctcac
     6601 cccagggtta actattatca gaagggtact ataaagcgtt gtcgaacaaa tctacataca
     6661 gacacgccct attatcatct attttcagac tctttttgtt taaattagtt tcgatgaccg
     6721 cgaaatgagt gctgtctcgg gcagcaaagt taagtagaat ccgcggcgac cattcgacaa
     6781 aagaggtgaa tcatgcagga tttatcattg gaagcacgcc tggcagagct ggagagccga
     6841 ctggcttttc aggaaatcac cattgaagaa ctgaacgtca cggtgaccgc tcatgaaatg
     6901 gagatggcga aactgcgcga tcatctgcgt ctgctgaccg agaagttaaa agccagccag
     6961 ccgtcgaaca tcgcgtcgca ggctgaagaa acgccaccgc cacattattg aggcgtaaaa
     7021 aaagcgggga ttccccgctt ttttgtcact ttttcggtat tagtggcaac cgcaaccgcc
     7081 gttgccttta ccgccacagc agccttcgcc accgtgttca tgaccgtgat cgtggccatg
     7141 accgccgcag caaccgtcgt ggtcgtgatc gtggtgatga tcgtgcgcgc cgtgaacgtg
     7201 accatgagcc agttcttctt cggtcgcttc acgaatcgcc acaacctcaa cgttgaattt
     7261 caggttctga ccggccagca tgtggttacc atcaaccact acgtgatcgt cttcaaccgc
     7321 agtgatttca accggtaccg gaccctggtc ggtttcagcc aggaaacgca tacctacctg
     7381 cagttcatca acgcccataa atacgtcttt aggaacacgt tgcaccaggt tttcgtcgta
     7441 ctgaccgtaa gcgtcgttcg cgccaacagc gacatcaaat ttgtcgccaa cttcatgacc
     7501 ttccagcgcc gtttccaggc cagagatcag ggaaccgtga ccatgcaggt agtccagcgg
     7561 cgcactcacc ggagactcat caaccaacac accgtcttct gtacgtacct gataggccag
     7621 gctgaccacc aggtcttttg ctactttcat gatatctcct gagcatggga agaatagtgg
     7681 cgcagattgt agcggaattc tgcagccgtg tactcactag cttaaaaaaa ctcggcgcat
     7741 atcgctagtc cggatgaaaa atcccgatca cttgctcatc tttgcgaacg tgatcgcggg
     7801 cttctttgtc tgcttctcgc atctgatgtc cgcacttaac gcattcaaca atatcaatat
     7861 tattttcgcg ccacatcgcc attgaatcct gtgcctgaca ggccgggcat tttgcgcccg
     7921 caataaaacg ttttcggatt gccatcttta ccctctactc aaattcatcc cagccgtcca
     7981 gctggcgtcg ttcttgttgc atttcacgct ggaaaatctc ttccagttcg cgtcgggctt
     8041 ccctggcgcg agaaatttgt acagtatcgg catgcattgg gatgagctct cgcagcatcc
     8101 gcatatccag gcggcgaaaa tgcagttgcg cccgctgcgc ctgatgcgga tgcatgccaa
     8161 gcgtgaccag cgtcttgcgc cccagctcta acgcactgga gaatgtttca cgggaaaact
     8221 gcgtcacccc tgcctgtaat aactcatgcg cttccacacg tccgcgcgct cgcgcaagaa
     8281 tatgcaaatg cggaaagtgc tgctggcata tttccaccag cttcatggtg tcttccggtt
     8341 cgttacaggt gatcacgata gactcagcgg cctctgcacc cgcagaacgt aaaagatcga
     8401 cctgcgtggc gtcgccgtaa taaactttgt agccgtattt acgcatcagg ttaacggcgc
     8461 tgatatcccg ctccagcacg gtaatgcgca ttttatttgc catcagcaaa cgaccaatca
     8521 cctgaccaaa acgcccgaag cccacgacaa tgacctgggg tttatcatcg ttgacccacg
     8581 gtttttcgtc ttcttcttcc ggtccgttaa actggcggga tagccattta tccaccagct
     8641 tcatcagcaa cggcgtggtc atcatggaaa gcgtcaccgt caccagcaac agcgccatct
     8701 ggtcgccctg gaataagcgt tgcgaagaag cggtagaaaa gaggacaaag gcaaactcgc
     8761 cgccctgact caacacgcca gcaaactgca tccgctctga gctacgcacg ccatacaatc
     8821 gcgccagcag atacaacacg agaattttca ccgccaccag cacaaccacg cttatcacta
     8881 cccacaacag atgggtataa agcaccccga gattgagcga catgccgaca gagataaaga
     8941 acaaaccgag cagcaagcct ttaaagggat cgatagccgt ttccagttca tggcgatatt
     9001 cactttccgc cagcagcacg cccgcaataa atgtaccgag cgccatcgac agccccagcg
     9061 catccataaa caatgcggaa cccaacacca gcagcagcgt cgcggcggtg aacacttccc
     9121 gcacgccaga agctgcaata aagcggaata ccggacgcag taaatagcgc ccaccaatca
     9181 gcatgccgac aaacgccagc accttcatgc cgatcttcat ccagtcgaaa tgttcgtctg
     9241 ccgaccccgc caacaacggc actaacgcca gtgccgggat caccgccaga tcctgaaaca
     9301 gcagtaccga gaaacctaac tggccggatt cgctgcgatt catccctttc tcacgcatca
     9361 attgcaacgc cattgcagtt gaagacatcg caaggccaat gccaccgacc accgccgcct
     9421 gccaggcgaa atccgtcagc atcaataatc ccgccagcaa cgccgcgctt aacagcacct
     9481 gtgccgcgcc tacgccaaaa atcgaacgcc gcagttgcca aagtttggag ggattcaact
     9541 caaggccgat gataaacatc aggaatacca cgccgagttc cgaaaagtgg aggatctcat
     9601 cgacgtcgct aataaacccc agcccccacg ggccaattgc aatccctgcc agcaaatatc
     9661 ccaacacagc gccaataccc agccgcgatg ccagcggcac cgcagccacc gccgcgaaga
     9721 gaaacagcac tcctgcgagt aaaaaatcgg aaccttccat cagcggcctc ctggagacag
     9781 cggatttgcc agccagtcac cgtaggctct ggcgtggctc gccagctcct gtgcgctttg
     9841 ccgtctcgcc cagtaaataa tgatgggact taaccaatgc atccggcaca tgcccgccgc
     9901 cagttcaaag gggcgcagca catcgctcat cgggtagcga ttcagcgcgt cataacggta
     9961 agcactttcc ggctcgccgg tggtaatcac gctacgccag tactttcccg ccagttggtt
    10021 tcctcccggc ccgctggcaa aaccacgact taatacccgg tccagccact ctttcagtag
    10081 cgccgggcag ctataggtat aaagaggatg ctgaaagaca atcacctcgt gctcgcgcag
    10141 taatgcctgc tcacggggga tatcaataaa aaaatcggga tagtgcgcgt aaaggtcgtg
    10201 cacggtaaca ttgctgagct gcgtggccgg tttaagcagt acccggtttg ccaccgagtc
    10261 ctgagattcc ggatgggcat acagcagcaa aactttcgct ggctgagaca tcatccccct
    10321 cccggtatgg tttttgtgta tagtcgctgt tttgggctac cattgcgccc ggtgcggcag
    10381 ctcgcccata cattacatta tcataatgat aagttaacat agtctgaaca tacggcacct
    10441 tatgattgtt ttctcctcgt tacaaattcg tcgcggcgtg cgcgtcctgc tggataatgc
    10501 caccgccacc atcaaccctg ggcagaaagt cggcctggtg ggtaaaaacg gctgtggtaa
    10561 atctaccctg ctggcattgc tgaaaaatga aatcagcgcc gacggcggca gctacacctt
    10621 tccgggaagc tggcaactgg cgtgggtgaa tcaggaaacg ccggcgttac cgcaagcggc
    10681 gctggaatat gtcattgacg gcgaccgtga atatcgtcaa ctagaagcgc agctacacga
    10741 cgccaacgaa cgtaacgacg ggcacgccat tgcgaccatt catggcaagc tggatgctat
    10801 tgacgcatgg agtattcgct cccgtgctgc cagcctgctg cacggcctcg gtttcagcaa
    10861 tgaacaactg gagcgcccgg taagtgattt ctccggtggc tggcgtatgc gtcttaacct
    10921 tgcccaggcg ctgatttgcc gttcagactt gctgctgctc gacgaaccga ctaaccacct
    10981 cgatctcgat gccgttatct ggctggaaaa atggttgaag agctatcagg gcacgctgat
    11041 cctgatctct cacgaccgcg acttcctcga tccgatcgtt gataaaatta ttcatatcga
    11101 acaacaaagc atgttcgagt acaccggcaa ctacagttcg tttgaagtac agcgcgccac
    11161 ccgtctggcg cagcaacaag cgatgtatga aagccagcag gaacgcgtag cgcatctgca
    11221 aagttatatc gaccgtttcc gtgccaaagc caccaaagcg aagcaggccc agagccgcat
    11281 taagatgctc gagcgtatgg agctgattgc ccccgcgcac gtcgacaacc cgttccgctt
    11341 tagcttccgc gcgccggaaa gcctgccaaa tccgttactg aagatggaaa aagtcagcgc
    11401 gggctatggc gatcgcatta ttctcgactc gattaaactg aacctggtgc ccggctcgcg
    11461 cattggtctg ttaggccgca acggcgcggg taaatcgaca ttaatcaaac tgttagccgg
    11521 tgaacttgcg ccagtcagcg gtgaaattgg tctggcgaaa gggatcaagc tcggctactt
    11581 cgcccagcat caacttgaat acctgcgcgc cgacgaatca cctattcaac atctggcacg
    11641 tttagcgccg caggagctgg aacaaaaact gcgtgactac ctcggcggct ttggtttcca
    11701 gggcgataaa gtaaccgaag aaacgcgccg cttctcaggt ggggaaaaag cccgcctggt
    11761 gctggcatta atcgtctggc agcgtccgaa tctgctgctg ctcgacgaac cgactaacca
    11821 ccttgacctc gacatgcgtc aggcactcac cgaagcatta atcgagtttg aaggcgcgct
    11881 ggttgtcgtt tcgcacgacc gtcatttgct gcgttccacc actgacgatc tctacctggt
    11941 tcacgatcgt aaagtcgaac cgttcgacgg cgatctggaa gattatcaac agtggttgag
    12001 cgacgtacaa aagcaggaaa accaggccga cgaagcgcca aaagagaacg cgaacagcgc
    12061 ccaggcacgt aaagatcaga agcgccggga agcggagctg cgtgcgcaaa cccagccact
    12121 gcgtaaagag attgcccgtc tggaaaaaga gatggagaag ctgaacgcgc aactggcgca
    12181 ggcggaagag aaactcggcg acagcgaact gtatgaccag agccgtaaag cggagttgac
    12241 cgcctgcctg caacagcaag ccagcgccaa atccggcctg gaagagtgcg aaatggcgtg
    12301 gctggaagcc caggagcagc ttgagcagat gttgctggaa ggccaaagca actgatggcg
    12361 cagataacga cgaccgatgc caatgaattc agcagcagtg ctgaattcac ccctatgcgc
    12421 ggctttagca attgtcatct gcaaaccatg ctgccgcgtc tgtttcgtcg caaggtgaaa
    12481 ttcaccccgt actggcagcg gctggagttg cccgacggcg attttgtcga tctcgcgtgg
    12541 agtgaagccc cagcacaggc gcggcataaa ccgcgtttag tggtatttca cgggctggaa
    12601 ggcagtctca atagccctta cgcccacggt ctggttgagg cggcgcaaaa gcgcggctgg
    12661 ctgggcgtgg tgatgcattt tcgcggatgc agcggtgaac caaaccgtat gcaccgcatt
    12721 taccattcgg gcgaaaccga agacgccagt tggtttttac gctggctgca acgcgaattt
    12781 ggtcatgcgc caacggctgc cgtcggctat tcgctcggcg gtaatatgct ggcctgtttg
    12841 ctggcaaagg aaggcaatga tctcccggtt gatgcggcgg tgattgtctc tgcgccgttt
    12901 atgctggaag cctgtagcta tcatatggaa aagggctttt cccgcgttta tcagcgttac
    12961 ttgctgaacc tgttaaaagc caatgccgcg cgcaagctgg cagcctaccc cggaacgctg
    13021 ccgattaatc tcgcgcagtt aaaatcggta cgtcgcatcc gtgaatttga cgatctaatc
    13081 accgccagaa ttcacggcta cgccgacgct atcgactatt atcgtcagtg tagcgccatg
    13141 ccgatgctga accggatcgc caaaccgacg ctgattattc acgccaaaga cgatccgttt
    13201 atggatcatc aggtgatccc gaaaccggaa agtctccccc cgcaggtgga gtatcaactg
    13261 actgaacatg gcggtcatgt tggctttatt ggcggtacat tacttcatcc gcaaatgtgg
    13321 ctggagtcac gcattcctga ctggttaaca acgtatctgg aggcgaaatc atgttgattc
    13381 cgtggcaaga cctctccccc gaaacgctgg aaaatttaat tgaaagcttt gtgttacgtg
    13441 aaggcaccga ttatggtgaa catgagcgta cacttgaaca gaaagtcgcc gacgtcaaac
    13501 gccagctaca gtgcggagaa gcggtgctgg tatggtcgga gctgcacgaa acggtcaata
    13561 ttatgccgcg cagtcagttt cgcgaataac ctgcaaccgt cggcggacga gataataatg
    13621 atagctaagt catggagtta ctatgtctgc caaacatccg gtcattgcgg taacaggatc
    13681 cagcggcgcg gggaccacca ccaccagcct cgcgtttcgt aaaatattcg cgcagttaaa
    13741 tctgcatgca gctgaggtgg aaggcgacag ttttcaccgt tacacccgcc cggaaatgga
    13801 catggcgatc cgcaaagcgc gcgacgccgg gcggcatatc agctacttcg gccccgaagc
    13861 gaacgacttc ggcctgctgg aacaaacctt cattgaatac ggtcagagcg gcaaagggaa
    13921 atctcgcaaa tatctgcata cctacgatga agccgtaccg tggaatcagg taccgggaac
    13981 attcaccccc tggcaacctt taccagaacc cacagatgta ctgttttatg aaggtttaca
    14041 cggcggcgta gtcacgccac agcataacgt tgcgcagcat gtggacttac tggtcggcgt
    14101 ggtgcctatc gttaaccttg agtggattca aaaactgatc cgcgacacca gcgagcgcgg
    14161 gcactcacga gaagcagtga tggactcagt agtgcgttca atggaagact atatcaacta
    14221 catcacaccg cagttttccc gcacccatct taacttccag cgcgttccca ccgtcgacac
    14281 ttcaaacccg ttcgcggcaa aaggtatccc gtcgctggat gaaagctttg tggtgatcca
    14341 ttttcgtaat ctggaaggga tcgatttccc ctggctgctg gcgatgttgc aaggctcatt
    14401 catttcccac atcaatacgt tagtggtgcc cggcggcaaa atgggtctgg caatggaatt
    14461 aattatgctg ccgctggtgc aacgattgat ggaaggaaag aaaatcgagt aactctgcta
    14521 ttacgccgga taaaatacta tccggcttca caacgggata gttaaatcac gcgccaacca
    14581 cttcatacga gtgagtaata ttcaccgctt tttccagcat caacgccact gaacaatatt
    14641 tctcggcaga gagatcaacc gcacgcgcaa ccgctgcgtc tttcaggtcg cgaccggtga
    14701 cgataaaatg cagattaatg tgcgtaaaca ggcgtggtgc ctcttcgcgg cgttcagagg
    14761 tcaattttac ttcacaatcg accacatcct gacgcccttt ttgcaggatc gaaaccacat
    14821 cgatggcact gcaaccaccc gccgccatca acaccatttc catcggactt ggtgctttat
    14881 cgcctgagtt gccgtccatt aaaatctggt gaccagaggc agattcgccc agaaaagtta
    14941 acccttcgac ccacttcact cgcgcttgca tttttgctac tccactgcgt caattttcct
    15001 gacagagtac gcgtactaac caaatcgcgc aacggaaggc gacctgggtc atgctgaagc
    15061 gagacaccag gagacacaaa gcgaaagcta tgctaaaaca gtcaggatgc tacagtaata
    15121 cattgatgta ctgcatgtat gcaaaggacg tcacattacc gtgcagtaca gttgatagcc
    15181 ccttcccagg tagcgggaag catatttcgg caatccagag acagcggcgt tatctggctc
    15241 tggagaaagc ttataacaga ggataaccgc gcatggtgct tggcaaaccg caaacagacc
    15301 cgactctcga atggttcttg tctcattgcc acattcataa gtacccatcc aagagcacgc
    15361 ttattcacca gggtgaaaaa gcggaaacgc tgtactacat cgttaaaggc tctgtggcag
    15421 tgctgatcaa agacgaagag ggtaaagaaa tgatcctctc ctatctgaat cagggtgatt
    15481 ttattggcga actgggcctg tttgaagagg gccaggaacg tagcgcatgg gtacgtgcga
    15541 aaaccgcctg tgaagtggct gaaatttcgt acaaaaaatt tcgccaattg attcaggtaa
    15601 acccggacat tctgatgcgt ctgtctgcac agatggcgcg tcgtctgcaa gtcacttcag
    15661 agaaagtggg caacctggcg ttcctcgacg tgacgggccg cattgcacag actctgctga
    15721 atctggcaaa acaaccagac gctatgactc acccggacgg tatgcaaatc aaaattaccc
    15781 gtcaggaaat cggtcagatt gtcggctgtt ctcgtgaaac cgtgggacgc attctgaaga
    15841 tgcttgaaga tcagaacctg atctccgcac acggtaaaac catcgtcgtt tacggcactc
    15901 gttaatcccg tcggagaggc gcgttacctg gtagcgcgcc attttgtttc ccccgatgtg
    15961 gcgcagactg atttatcacc ccgatatcaa ctatgcactt cgacaaacgc tggtgctatg
    16021 tttgcccgtg gccgttgggt taatgcttgg cgaattacga ttcggtctgc tcttctccct
    16081 cgttcctgcc tgttgcaata ttgcgggcct tgatacgcct cataaacgtt ttttcaaacg
    16141 cttaatcatt ggtgcgtcgc tgtttgccac ctgtagctta ctgacacagc tgctactggc
    16201 aaaagatgtt cccctgccct ttttgctgac cggattaacg ctggtacttg gcgtcactgc
    16261 tgagctgggg ccattgcacg caaaattgct tcctgcatcg ctgctcgccg ccatttttac
    16321 cctcagtttg gcgggataca tgccggtctg ggaaccgttg ctcatctatg cgttgggcac
    16381 tctctggtac ggattgttta actggttttg gttctggatc tggcgcgaac aaccgctgcg
    16441 cgagtcacta agtctgctgt accgtgaact ggcagattat tgtgaagcca aatacagcct
    16501 gcttacccag cacaccgacc ctgaaaaagc gctgccgccg ctgctggtgc gccagcaaaa
    16561 agcggtcgat ctaattaccc agtgctatca gcaaatgcat atgctttccg cgcaaaataa
    16621 tactgactac aagcggatgc tgcgtatttt ccaggaggcg ctggatttac aggaacatat
    16681 ttcggtcagt ttgcatcagc cggaagaggt gcaaaagctg gtcgagcgta gccatgcgga
    16741 agaagttatc cgctggaatg cgcaaaccgt cgccgctcgc ctgcgcgtgc tggctgatga
    16801 cattctttac catcgcctgc cgacgcgttt tacgatggaa aagcaaattg gcgcactgga
    16861 aaaaatcgcc cgccagcatc cggataatcc ggttgggcaa ttctgctatt ggcacttcag
    16921 ccgcatcgcc cgcgtgctgc gcacccaaaa accgctctat gcgcgtgact tactggccga
    16981 taaacagcgg cgaatgccgt tacttccggc gctgaaaagt tatctgtcac taaagtctcc
    17041 ggcgctacgc aatgccggac gactcagtgt aatgttaagc gttgccagcc tgatgggcac
    17101 cgcgctgcat ctgccgaagt cgtactggat cctgatgacg gtattgctgg tgacgcaaaa
    17161 tggctatggt gcaacccgtc tgaggattgt gaatcgctcc gtgggaaccg tggtcgggtt
    17221 aatcattgcg ggcgtggcgc tgcactttaa aattcccgaa ggttacaccc tgaccttgat
    17281 gctgattacc acccttgcca gctacctgat attgcgcaaa aactacggct gggcgacggt
    17341 cggttttact attaccgcag tgtataccct gcaactgttg tggctgaacg gtgagcagta
    17401 catccttccg cgtcttatcg ataccattat tggttgttta attgctttcg gtggtaccgt
    17461 ctggctgtgg ccgcagtggc agagcgggtt attgcgtaaa aacgcccatg acgctttaga
    17521 agcctatcag gaagcgattc gcttgattct gagcgaggat ccgcaaccta cgccactggc
    17581 ctggcagcga atgcgggtaa atcaggcaca taacactctg tataactcat tgaatcaggc
    17641 gatgcaggaa ccagcgttca acagccatta tctggcagat atgaaactgt gggtaacgca
    17701 cagtcagttt attgttgaac atattaatgc catgaccacg ctggcgcggg aacaccgggc
    17761 attgccacct gaactggcac aagagtattt acagtcttgt gaaatcgcca ttcagcgttg
    17821 tcagcagcga ctggagtatg acgaaccggg tagttctggc gatgccaata tcatggatgc
    17881 gccggagatg cagccgcacg aaggcgcggc aggtacgctg gagcagcatt tacagcgggt
    17941 tattggtcat ctgaacacca tgcacaccat ttcgtcgatg gcatggcgtc agcgaccgca
    18001 tcacgggatt tggctgagtc gcaagttgcg ggattcgaag gcgtaatgca ggctaaacca
    18061 tcggataagg cattcacgcc gcatccgaca tcttttgcct gatgcgtctt atttacgccc
    18121 caaccacctt cgccaccgcg tgggcgaaac gttgcatccc ttcatcgata tccgcatctt
    18181 ccaccaccag cgacggtgca aaacgcatca catccggtcc ggcattcagc accattacgc
    18241 cagcctctgc gcccgcatac aggaaatcac gcgcctgacc tttgtactgt ggtttcagct
    18301 ccgcgccaat taacagcccc ataccgcgaa tatcgctaaa cacatcgtac tgctgatcga
    18361 tcttctgcaa atggtcaaca aaacgctggc gtttcgcctg aatgccttcc agcacttcag
    18421 gggtattaat gatatcaaac gccgcgcccg ctactgcaca ggccagagga ttaccgccgt
    18481 aggtggaacc gtgagaacca ggatgaaacg cagaagcaat ttccgccgtg gtcagcatgg
    18541 cgctaatcgg gaagccgccg cctaacgctt tcgcagaagt cagaatatcc ggcgtcacgc
    18601 cgtagtgcat gtaagcaaac aaatcgccag tccgccccat cccgcactgt acttcatcaa
    18661 acaccaataa tgcctgatgt tgatcgcaca actcacgcaa gccctgcaaa aactctggcg
    18721 tcgctgccgt cacaccgccc tcgccctgga tcggctcaac caccactgca caggtgtgat
    18781 catccatcac cgctttcact gcgtggagat cgttaaaggg aacgtggatg atgtctgccg
    18841 gttttggccc aaagccgtcg gaatattttg gctgcccacc caccgaaacg gtaaacagcg
    18901 agcgaccatg aaaagcgtta tggaaggcaa taattttggt tttgaacggg ctatgacgca
    18961 cacaggcgta atggcgtgcc agtttaaagg cggtttcgtt agcttccgtg ccggagttca
    19021 taaacaccac gcgttcggca aacgtcgcct caatcagttt atgcccaaga cgcagcgccg
    19081 gttcattggt gaaaacgtta ctgatatgcc acagagtttc gccctgggtt tttaacgcgt
    19141 tcaccagcgc aggatggcaa tggcccaacg ccgtaactgc aatgccaccc gcgaaatcga
    19201 catactcctt gccttgctga tcccagattc ggctgccctg accttttacc ggaataaact
    19261 ctgccggtgc ataaatcggc aggatcactt catcgaaagt cgcgcgtgta attgctgttt
    19321 gttcaattgc catctcatga tcaccctgtt acgcataaat aaatttgaaa ttataatcac
    19381 aaaatatgca taaaaaatca ctgaatggca atcagaaatc agcgatgcag gaaattagcc
    19441 agcagttgat gtccttgttc gctaagaata ctttctggat ggaactgcac accttccaga
    19501 tcccactggc gatggcgaat ccccataatc tcgcgggttt cgctccaggc cgtcacgtca
    19561 aagcacgctg gtaatgagtc aggttccacc accagcgaat ggtagcgtgt cacggtaagt
    19621 ggatttgcca gcccccgaaa tacgccctca ccgttatgtg taatcggcga ggttttgccg
    19681 tgcatgacct ttgcggcgcg cacaacttta ccgccaaatg cctgcgccat tgcctgatga
    19741 ccgaggcaga cgccaagaat cggcaagcgc ccggcatagt ggcgaataac gtcaagggag
    19801 atcccggctt catctggcgt acaggggcca ggtgagatga caattttttg tggtttaagg
    19861 gcgtcgatat ccgccagcgt caacgcatcg ttgcgcttaa ccagcacatc cgcccccagt
    19921 tcacaaaagt actggtagag gttccaggta aaagaatcgt agttatctat aagcaggatc
    19981 atggcggctc cggtacgaaa gaaccacgct attttactca gattccccgg cttcgcttac
    20041 cactttgcta aatatcatct gcagcgcggt gagatccccc attgccccac tctgatttgc
    20101 ctgattccag gcttcttttt cgataccctg ccagctcagt tgatatccgg catgaatcgc
    20161 cagttgctcg aagaagatcc gctgtgccag tccgcttccc acacggaagg gatgcagcac
    20221 gttgatttca caatagtaat gcgccagccg ctcgacgaac ttcgctttct ccaggccaac
    20281 cagataacct tcttcctcca gatcctgcat cagggcattg ccctcttttt cgatataagc
    20341 aaagtggcag aacggcgtat cgccctgata aatatcaact tcacgcagtt gccccgccca
    20401 gtcgaaaata tcctgataca gctggcgatg gatagtgcgc aaatgcggta aaccgcgcac
    20461 cagcggacca agctcaatgg tcgcagcacg cagcgccgtc atttcgtaag cggcctgttc
    20521 cagccgctgc tgctggtgga tgttcagccg gttacgcatg atatcaaggc caggataaag
    20581 atacggatcg cgcccttcgc cgaatttatc gctcatagtg cctcctcagc tcttcaaggc
    20641 gcgctaaagc ctctgcggca gtaagagtga ctaaaggcat ctcgacgcct tcaagacggc
    20701 gactggcctg gaaattacga ttacgctgaa gctcccagag acgggacttt tgcttatcgg
    20761 tgagtttctt cacttgatgc ctccctgaac gtgtccattt gccacaagta taagcggcaa
    20821 atccgggtta cgccgggaga gcagaagcgc gggcaagatt gcccgcgcga aaaatcatta
    20881 cggcaggact ttagcggaaa ggataactac cggttttgac ggcacattct ggtacggacc
    20941 aacgtcatga gtcggcacct gggaaatctt atcggcaacg tccatgcctt tcaccacttt
    21001 accaaatacc gcgtaaccga aatcacgctg accatggtca aggaaggcgt tatcggcaac
    21061 gttgataaag aactggctgg tggcgctgtc tttgtcagcg gtacgcgcca tcgcgatggt
    21121 gccacgcgtg ttgcgcaggc cgttatcggc ttcatttttg attggcgggt ttggtttttt
    21181 ctgctgcatc tgctcggtga aaccgccgcc ctgaatcata aagccaggaa tgacgcggtg
    21241 aaaggtagtg ttgttataaa aaccgctgtt cacataatcg acaaagtttt gcacagacac
    21301 tggcgctttt tgtttatcca gctccagttc gatgttacca gctgaggttg tcaacaatac
    21361 gtgcgggtcc cctttcgctg ccattgctgc gggagaaaga gcagaaagag cgaaaacagc
    21421 agccatcgcc gccagggtcg atttgaacat gagatttcct taacaaacag agcagaaaaa
    21481 aagcaagtga agagattcta aatagccttc aagaacaagg ccatcccttt acctaatttt
    21541 acgtatctga aatatatgta acctgacgat cactttttat tccgtgatca aaatcacctc
    21601 ttaaaatgca atttagcaac cgattgcaat aaaacattta aacagatcac aaaatcacct
    21661 aaaatcgccc gctcgcggca tatcccgcta tgccatttta tttttgaacg caggccaatc
    21721 atgactaaca gcaatcgcat caagctcaca tggattagct ttctctccta cgcactgact
    21781 ggtgcgttgg ttattgtcac cgggatggtg atgggaaata tcgccgatta tttcaatctg
    21841 cctgtttcca gtatgagtaa caccttcacc ttcctcaacg ccggcatttt aatctctatc
    21901 ttcctcaacg cctggctgat ggaaatcgtc ccgttgaaaa cgcagttacg ttttggcttt
    21961 ctcctgatgg tgctggcggt tgccggtttg atgttcagcc acagcctggc gctgttctcg
    22021 acggcgatgt tcattctcgg ggtggtcagc ggcattacta tgtcgattgg tacattcctg
    22081 ataacacaaa tgtatgaagg gcgtcagcgc ggttcccgcc tgttatttac tgactccttc
    22141 ttcagtatgg ctgggatgat tttcccaatg atcgccgcgt ttctactggc gcgcagcatt
    22201 gagtggtact gggtttatgc ctgcatcggg ctggtgtatg tcgctatttt tattctgacc
    22261 ttcggctgtg agttcccggc actgggcaaa catgcgccaa aaacggatgc tccggtagag
    22321 aaagaaaagt gggggatcgg cgtactgttt ctctctgttg cggcgctgtg ctacatcctc
    22381 ggtcagttag gttttatctc ctgggtgcct gagtatgcca aaggcctggg catgagcctg
    22441 aacgacgcgg gcacgctggt gagtaacttc tggatgtcat acatggtcgg catgtgggcg
    22501 ttcagcttta ttcttcgctt ctttgatttg caacgcattc tgaccgtact ggctggtctg
    22561 gctgcgattc tgatgtacgt ctttaacacc ggaacaccag cacatatggc gtggtcaatt
    22621 ctcgctctgg gcttcttctc cagcgcgatc tataccacca tcatcaccct gggttcacag
    22681 cagaccaaag taccgtcgcc aaaactggtt aactttgtcc tgacctgcgg aaccatcggt
    22741 actatgttga cctttgtggt taccggcccg attgttgaac atagcggtcc gcaggcggca
    22801 ctgcttacag caaacggtct gtacgctgtc gtctttgtga tgtgcttcct gttaggtttc
    22861 gtcagccgtc accgtcagca taacaccctg acctctcatt aattgctcat gccggacggc
    22921 actatcgtcg tccggccttt tcctctctta cccccgctac gtgcatctat ttctataaac
    22981 ccgttcattt tgtctatttt ttgcacaaac atgaaatgtc agacaattcc gtgacttaag
    23041 aaaatttata caaatcagca atatacccct taaggagtat ataaaggtga atttgattta
    23101 catcaataag cggggttgct gaatcgttaa ggtaggcggt aatagaaaag aaatcgaggc
    23161 aaaaatgagc aaagtcagac tcgcaattat cggtaacggt atggtcggcc atcgctttat
    23221 cgaagatctt cttgataaat ctgatgcggc caactttgat attaccgttt tctgtgaaga
    23281 accgcgcatc gcttatgacc gcgtacacct ctcgtcttac ttctctcacc acaccgccga
    23341 agagctgtcg ctggtgcgcg aaggcttcta cgagaaacac ggcatcaaag ttctggtcgg
    23401 cgaacgcgct atcaccatca accgtcagga gaaggtgatt cactccagcg ccgggcgcac
    23461 cgttttttat gacaagctga tcatggcgac cggttcctac ccgtggatcc cgccaatcaa
    23521 aggttctgat actcaggact gctttgtcta tcgcactatt gaagacctca acgccattga
    23581 atcctgcgcc cgtcgcagca aacgcggtgc cgttgttggt ggcggcctgt taggtctgga
    23641 agccgctggc gcgctgaaaa acttaggtat tgaaacccac gttatcgaat ttgcccctat
    23701 gctgatggcg gaacagcttg atcagatggg tggcgagcag ctgcgtcgca aaatcgaaag
    23761 tatgggcgtg cgcgttcaca ccagcaaaaa cacccttgag attgtgcagg aaggtgtcga
    23821 agcgcgtaaa accatgcgtt ttgccgacgg cagcgaattg gaagtcgact ttatcgtctt
    23881 ctctaccggt atccgtccgc gcgataagct ggcaacccag tgtggtctgg acgttgctcc
    23941 gcgtgggggt attgtcatta atgattcctg ccagacttcc gatccggata tctacgccat
    24001 cggtgaatgc gcaagctgga acaaccgtgt atttggtctg gtagcaccag gctacaaaat
    24061 ggcgcaggtc gccgttgacc atattctcag tagcgaaaac gcctttgaag gtgctgacct
    24121 tagcgccaag ctgaaactgc tgggcgtaga cgtaggcggt attggtgatg cgcacggtcg
    24181 cacgcctggc gcacgtagct atgtttacct cgacgaaagc aaagagatct acaaacgcct
    24241 gattgtcagc gaagacaaca aaaccctgct cggtgcggta ctggtgggcg ataccagcga
    24301 ctacggcaac ctgctgcaac tggtgctgaa cgctatcgag ctgccggaaa acccggattc
    24361 tctgattctg ccggcgcact cgggtagcgg taagccgtct atcggtgttg ataaactgcc
    24421 ggacagcgcg caaatctgct cctgcttcga cgtcaccaaa ggtgatctga ttgctgccat
    24481 caacaaaggc tgccacaccg ttgcggcgct gaaagccgaa accaaagcgg gtactggctg
    24541 cggtggctgt atcccgctgg tcactcaggt actgaacgcg gaactggcga aacagggcat
    24601 cgaagttaac aacaacctgt gcgaacactt tgcttattcg cgtcaggaac tgttccattt
    24661 gatccgcgtt gaaggcatta aaaccttcga agaactgctg gcgaaacacg gcaaaggcta
    24721 cggttgtgaa gtttgtaaac cgaccgtcgg ttcgctgctg gcctcctgct ggaacgaata
    24781 cattctgaag ccggaacata ctccgctgca ggattctaac gacaacttcc tcgctaacat
    24841 ccagaaagac ggcacctact cggtgatccc gcgctctccg ggcggtgaaa tcaccccgga
    24901 agggctgatg gcagtgggtc gtatcgcgcg tgaatttaat ctctacacca aaatcaccgg
    24961 ttcccagcgc ctggcgatgt ttggcgcaca gaaagacgat ctgccggaga tctggcgtca
    25021 gctgattgaa gccggcttcg aaaccggtca tgcctatgcg aaagcactgc gtatggcgaa
    25081 aacctgcgtg ggtagcacct ggtgccgcta cggcgttggc gacagcgtcg gcctcggcgt
    25141 ggaactggaa aaccgctaca aaggcatccg tacgccgcac aaaatgaagt tcggtgtctc
    25201 cggctgtacc cgtgaatgtt cagaagctca gggtaaagac gtgggtatta tcgccactga
    25261 aaaaggctgg aacttgtatg tttgcggtaa cggcggcatg aaaccgcgtc atgcggatct
    25321 gctggcagcg gatatcgatc gcgaaacgct gatcaaatat ctcgaccgct tcatgatgtt
    25381 ctacatccgt accgccgaca aactgacgcg taccgcgccg tggttagaaa acctcgaagg
    25441 cggcatcgat tatctgaaag cggtgatcat tgacgacaaa ctggggctga acgctcatct
    25501 ggaagaagag atggcgcgcc tgcgtgaagc ggtagtgtgt gagtggactg aaacggtcaa
    25561 tacgccgtct gcgcagactc gcttcaaaca cttcatcaac agcgacaagc gtgacccgaa
    25621 cgtgcagatg gtgccagagc gcgaacagca ccgtccggca acgccgtatg aacgtatccc
    25681 ggtaactctg gtggaggaca acgcatgagc cagtggaaag acatctgcaa aatcgatgac
    25741 atcctgcctg aaaccggcgt ctgcgcgctg ttaggtgacg agcaggtagc gattttccgc
    25801 ccgtatcaca gcgaccaggt gtttgcgatc agcaacatcg atccgttctt cgagtccagc
    25861 gtgctatcac gcgggctgat tgcggaacac cagggcgagc tgtgggtcgc cagcccgctg
    25921 aaaaaacagc gttttcgctt aagtgatggc ttgtgcatgg aagacgaaca gttttccgtc
    25981 aaacattacg aagcacgagt gaaagacggc gtggtgcagc tgcgcggtta atgttttaac
    26041 gggaggcgca atgcctcccc tttttgcatg gtcctg
//
LOCUS       DAPHBJ010000050        26000 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.050, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000050 DAPHBJ010000000
VERSION     DAPHBJ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.050"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(57..137)
                     /gene="shoB"
                     /locus_tag="RHZ76_001838"
     CDS             complement(57..137)
                     /gene="shoB"
                     /locus_tag="RHZ76_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HDV2812490.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(558..938)
                     /gene="acpS"
                     /locus_tag="RHZ76_001839"
     CDS             complement(558..938)
                     /gene="acpS"
                     /locus_tag="RHZ76_001839"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDV2812491.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGDRLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLADERHYACATVIIES"
     gene            complement(938..1669)
                     /gene="pdxJ"
                     /locus_tag="RHZ76_001840"
     CDS             complement(938..1669)
                     /gene="pdxJ"
                     /locus_tag="RHZ76_001840"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HDV2812492.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1681..2409)
                     /gene="recO"
                     /locus_tag="RHZ76_001841"
     CDS             complement(1681..2409)
                     /gene="recO"
                     /locus_tag="RHZ76_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDV2812493.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2421..3326)
                     /gene="era"
                     /locus_tag="RHZ76_001842"
     CDS             complement(2421..3326)
                     /gene="era"
                     /locus_tag="RHZ76_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDV2812494.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(3323..4003)
                     /gene="rnc"
                     /locus_tag="RHZ76_001843"
     CDS             complement(3323..4003)
                     /gene="rnc"
                     /locus_tag="RHZ76_001843"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDV2812495.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(4275..5249)
                     /gene="lepB"
                     /locus_tag="RHZ76_001844"
     CDS             complement(4275..5249)
                     /gene="lepB"
                     /locus_tag="RHZ76_001844"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDV2812496.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGLRLSRIGGIH"
     gene            complement(5265..7064)
                     /gene="lepA"
                     /locus_tag="RHZ76_001845"
     CDS             complement(5265..7064)
                     /gene="lepA"
                     /locus_tag="RHZ76_001845"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDV2812497.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGMQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(7262..7741)
                     /gene="rseC"
                     /locus_tag="RHZ76_001846"
     CDS             complement(7262..7741)
                     /gene="rseC"
                     /locus_tag="RHZ76_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HDV2812498.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7738..8694)
                     /gene="rseB"
                     /locus_tag="RHZ76_001847"
     CDS             complement(7738..8694)
                     /gene="rseB"
                     /locus_tag="RHZ76_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HDV2812499.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(8694..9344)
                     /gene="rseA"
                     /locus_tag="RHZ76_001848"
     CDS             complement(8694..9344)
                     /gene="rseA"
                     /locus_tag="RHZ76_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HDV2812500.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(9377..9952)
                     /gene="rpoE"
                     /locus_tag="RHZ76_001849"
     CDS             complement(9377..9952)
                     /gene="rpoE"
                     /locus_tag="RHZ76_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HDV2812501.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(9949..10104)
                     /gene="rseD"
                     /locus_tag="RHZ76_001850"
     CDS             complement(9949..10104)
                     /gene="rseD"
                     /locus_tag="RHZ76_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HDV2812502.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLILCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            10360..11982
                     /gene="nadB"
                     /locus_tag="RHZ76_001851"
     CDS             10360..11982
                     /gene="nadB"
                     /locus_tag="RHZ76_001851"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDV2812503.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIISDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDVRGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHDISTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(11967..12704)
                     /gene="trmN"
                     /locus_tag="RHZ76_001852"
     CDS             complement(11967..12704)
                     /gene="trmN"
                     /locus_tag="RHZ76_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HDV2812504.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECSTPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12836..14170
                     /gene="srmB"
                     /locus_tag="RHZ76_001853"
     CDS             12836..14170
                     /gene="srmB"
                     /locus_tag="RHZ76_001853"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HDV2812505.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(14379..15260)
                     /locus_tag="RHZ76_001854"
     CDS             complement(14379..15260)
                     /locus_tag="RHZ76_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2812506.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDVLLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            15363..15950
                     /gene="eamB"
                     /locus_tag="RHZ76_001855"
     CDS             15363..15950
                     /gene="eamB"
                     /locus_tag="RHZ76_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HDV2812507.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            complement(16006..16389)
                     /gene="grcA"
                     /locus_tag="RHZ76_001856"
     CDS             complement(16006..16389)
                     /gene="grcA"
                     /locus_tag="RHZ76_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HDV2812508.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            16694..17383
                     /gene="ung"
                     /locus_tag="RHZ76_001857"
     CDS             16694..17383
                     /gene="ung"
                     /locus_tag="RHZ76_001857"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDV2812509.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            complement(17431..18468)
                     /locus_tag="RHZ76_001858"
     CDS             complement(17431..18468)
                     /locus_tag="RHZ76_001858"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDV2812510.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            18675..19094
                     /gene="trxC"
                     /locus_tag="RHZ76_001859"
     CDS             18675..19094
                     /gene="trxC"
                     /locus_tag="RHZ76_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HDV2812511.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            19163..19861
                     /gene="tapT"
                     /locus_tag="RHZ76_001860"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             19163..19861
                     /gene="tapT"
                     /locus_tag="RHZ76_001860"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HDV2812512.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLELVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            19893..22553
                     /gene="pat"
                     /locus_tag="RHZ76_001861"
     CDS             19893..22553
                     /gene="pat"
                     /locus_tag="RHZ76_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HDV2812513.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQRKMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            22667..24022
                     /gene="pssA"
                     /locus_tag="RHZ76_001862"
     CDS             22667..24022
                     /gene="pssA"
                     /locus_tag="RHZ76_001862"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HDV2812514.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            24068..24391
                     /locus_tag="RHZ76_001863"
     CDS             24068..24391
                     /locus_tag="RHZ76_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HDV2812515.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDHSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(24388..25686)
                     /gene="kgtP"
                     /locus_tag="RHZ76_001864"
     CDS             complement(24388..25686)
                     /gene="kgtP"
                     /locus_tag="RHZ76_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HDV2812516.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
BASE COUNT         6274 a         6859 c         6489 g         6378 t
ORIGIN      
        1 tattaaaggt gcgtccgact ggttcaccgg acgcacctta agtacgtttc cttgtgttat
       61 aagaacagaa ggatcagctg taaaacagca atgatgattt tgatgacccg tttaatcagg
      121 tatcggcaat cagtcattcg tttttcctta aacaaggaat gcagccattc agattagccc
      181 ttacatctcc ccaaaactga acgtgcgagt tattgagggt gcatgctgca ctccacacca
      241 gagctttgac gacaccactc gtttcaatgg gggaattctg tggcatggtg taaagcacag
      301 caaaatcttc aataacgaag ccaattttaa tgtacttacg aattggcggt cacgttaatc
      361 tttccatcaa tattgctttc ttcgtaaagg ctcgagtttt tatgctaaag attgcaagtt
      421 gcttgtaaaa gataagtaca ctgatccata atcgctgttg ttgagggtgc atgctgcaca
      481 aaattaaagt taaaaagtaa aacccccgtt ccttaccagt tcgggggttt tactttttaa
      541 agagaacggt attattttta actttcaata attaccgtgg cacaagcata gtgccgctca
      601 tctgccagcg ttacatgcat atttgcaacg cccagctttt ccgccagttt taatgcctcg
      661 ccccatagcc gtagccgtgg tttgccgagc tcatcattga atacttcaaa ttgattaaac
      721 gccagaccat tgcggatccc ggtgccaaac gcttttgctg cggcttcttt cacagcaaaa
      781 cgcttcgcca gaaaacgcac cggctggtgg tgcgttttcc agatagccca ttcgttatcg
      841 cttaatacgc ggcgtgccag gcgatcaccg gatcgggcga tcaccgcttc gatgcgagcg
      901 atctccacaa tatccgtgcc taaacctaat attgccatta gccacgcgct tccagcatca
      961 gacgcttcat ttctgccacc gcatctttca gtccggtcat cactgcacga ccaataatgg
     1021 catgaccgat attcagttca tgcatctcag ggatggcggc aatggctttc acgttgtgat
     1081 aggtcagacc gtgtccggcg ttaactttca gaccgaggct tgcggcaaag gtcgcggctt
     1141 tggcgatacg cgccagctct tgcgcctgtt cggcgtcagt tttggcatca gcatagcaac
     1201 cggtgtggat ctcgataaac ggtgcgccaa cctctgccgc agctttgatc tgctcttcat
     1261 cggcgtcaat aaacagagaa acctgaatcc cggcatctgc cagacgtttg caggcatcgc
     1321 gcattttgtc acgctgccct gcgacatcca ggccgccttc ggttgttact tcctgacgct
     1381 tttccggtac caggcagcaa aaatgtggct tcgtctcaac ggcgatcgcc agcatctctt
     1441 cggtcaccgc catctccaga ttcatgcggg tatccagcgt ctgacgcagg atgcgcacgt
     1501 cgcggtcagt aatgtgacgg cgatcttcac gtaaatgcac ggtaatgccg tccgctcccg
     1561 cctgctcggc aataaacgcg gcctgcaccg gatccgggta agcggtaccg cgcgcgttgc
     1621 gcagcgtagc gatatggtca atgttgacgc ctaacagtaa ttcagccatg acaatcctca
     1681 tcattcataa tgtgttttca ccgttcgctt aggcataaat tgccggaaca gttccctgct
     1741 ctttaaaggt ttaccgccaa gatacggctt aagcgccatg cgggtaaagc gtttcgcggc
     1801 gcgcagtgtg tctgcgtcag gaaattcccg tgcgtttaac gcttttaact gccttccggt
     1861 gaacgtttta ttgtcgataa cgacgcttgc gataaaccct ttttcttcgc gataacgata
     1921 cgtcatggtg tcatctaccg gctcgccgct acccgcacaa tgggtaaaat tgacgccata
     1981 acccagatgc ccgagcagtg ccagttcaaa gcggcgcagc gcgggttctg gcgtaccagt
     2041 gacccctgca agagactgaa tgcagtgcaa gtaatcgaaa aaaagttcag agaagcgcgt
     2101 ctcgtattcc agtacgcggg agagaagttc gttgatgtac agaccgctgt aaagcgtgat
     2161 accgcttaat ggcagcgcca gcgagacggc ttcagcactg cgcagcgttt tgacttcgcc
     2221 acgcccgcca aaacgtagca agagaggggt gaaaggctgt aatgcacctt tcagggtaga
     2281 gcgtttagag cgtgcgcctt tggcaaccag acgcacgcgc cccgattcct ccgtgaagac
     2341 gtccagcatc aggctggttt cgctccacgg gcgactatgc aggacaaatg cgcgctgcca
     2401 gccttccatc ggagttactc ttaaagatcg tcaacgtaac cgagactgcg cagtgcgcgt
     2461 tcgtcgtcgg cccaaccgga tttcactttt acccacagtt caaggtgaac aggcgcttcg
     2521 aacatttcct gcatgtcttt acgcgcttca atcccgatgg ttttgatctt ggcccctttg
     2581 ttgccaatga ccatcttctt ctgcccttca cgctcaacga gaatcaaacc gttgatgtca
     2641 taaccaccgc gttcgttaga gacgaaacgt tcgatctcca cggtcacgga gtacggcagt
     2701 tcagcgccga ggaaacgcat cagtttttcg cggatgattt cagacgccat aaaacgctgt
     2761 gagcgatcgg tgatgtaatc ttccgggaag tgatgagtcg cttcaggtag atgcttacgc
     2821 acgattgccg caatagtgtc aacattcagc ccggtttcgg cagagattgg cacgatatcg
     2881 aggaagttca tctggcttgc caggaactgc aggtgcggca gcagatcggc tttctcctgc
     2941 acgttgtcca ctttgttcac cgcgaggatt accggcgctt tgccttcgcg cagtttgttg
     3001 agcaccattt cgtcgtccgg cgtccagcgg gtgccttcaa cgacaaaaat caccagctca
     3061 acatcgccaa tagagctgct cgccgctttg ttcatcaggc ggttaatggc gcgtttttct
     3121 tccatatgca ggcccggtgt atcgacgtag atcgcctgat acgcgccttc agtatggatc
     3181 cccacaatgc ggtgacgagt tgtctgcgcc ttgcgggaag tgatggagat tttctgcccc
     3241 agcagtttgt tcaacaatgt ggatttgcca acgttcggac gtccgacgat ggcaataaat
     3301 ccgcagtaac ttttatcgat gctcattcca gctccagttt tttcaacgcc tgttcggcgg
     3361 cagcctgctc agccttacga cggcttgaac ctgtgccaac caccggttca ctcaggccgc
     3421 tgacctggca gtggatagta aattcctgat cgtgcgcttc gccacgtacc tggactacca
     3481 gataagtcgg cagcggcaga tggcgaccct gcaaatattc ttgcaagcgc gttttcggat
     3541 ctttttgttt atcgcctggg ctaatttcgt ccaaacgagt ttgataccag ttgaggatta
     3601 atttctcgac ggtttgaata tcactgtcga ggaatacgcc accaattaat gcttcgacgg
     3661 tgtcggcgag aattgactca cgacgaaatc caccgctttt aagttcacct ggccctaaac
     3721 gtaagcactc gcctaactca aattcgcgcg ccagttccgc cagcgtattg ccacggacca
     3781 gcgtggcgcg catccggctc atatcgcctt catccacacg agggaaacgg tgataaagcg
     3841 cattggcgat aacgtagctc agaatagagt cgcctaaaaa ttctaaacgc tcgttatgtt
     3901 tactgctggc actacgatga gttaatgcct gctgcaacag ttcctgatga ttaaaagtgt
     3961 agcccagctt ccgttgaagc cgattaatta cgatggggtt catgcgatac caataaataa
     4021 atgcgtcaac aattcagcac acgaaacaga cctgatatac atggttctgc taactgcttc
     4081 gctgcagttt cagtttaccg gtatatgggg accaacgctg tttcgtgtgc cgtggcaacc
     4141 tggaggtgcc aaccttaaac ttcgggggaa tattctatac acaacgacgg gggatgtcgt
     4201 tagccacggg agatttatct cataaataat tcacgttgtc gccataacgg cgacaacgtg
     4261 aacgaagatg gctattaatg gatgccgcca atgcgactta agcgcagacc agtcggccat
     4321 tcgccttctt gcttatcgaa gctcatccag atagccgttg cccgaccgac cagattcgct
     4381 tccggcacaa agccccagta acggctgtcc gcgctgttgt cgcggttgtc gcccatcatg
     4441 aagtattgtc ccggaggaac aatccaggtt gccagttgtt gccctggctg ctggtaatac
     4501 atccccacct gatcctgcgc aatcggcact gtcagaatgc ggtgcgtcac atcacccagt
     4561 gtctctttac gctcggaaag acgaattcca ttttctttgg tttcgttttt cggcacttca
     4621 aagaatccgc tggtcgcttc cccaccatta cggcgtgaga aggtctgaac gaaatcgctc
     4681 ggttccacgt ttgagtaggt gaccggcagc gcgttttcac acgcctggcc ggaactgcat
     4741 cccggttgaa tcgtcagctc ttttgagacc ggatcgtaag tgactttatc gcccggtaaa
     4801 cccaccgcgc gcttgatgta atcaagcttt ggatcttccg gatatttaaa gaccacgata
     4861 tcgccgcgtt tcggatgacc ggtttcgatc agcgttttct ggtagatagg atctttaatg
     4921 ccataagcaa acttctctac cagaataaaa tcaccaatta acagagtcgg catcatcgaa
     4981 cctgacggga tctggaacgg ttcataaata aacgaacgca caatcaatac gatagccagt
     5041 accggaaaaa cagaagcacc ggtttccagc cagccaggct tcggcgcaac ctttttcaac
     5101 gttgctttat ccagtgagtc cccggcagcc gcctgcgccg ctgcctgacg ttcccgccgt
     5161 ttaggtgcga aaaagaattt atccacgcac cataaaatgc ccgtcaccag tgtggcaatc
     5221 accagaatca gggcaaacat attcgccatg ccaactccta agggttattt gttgtctttg
     5281 ccgacgtgca gaatggcgag gaacgcttcc tgcggcagct cgacgttacc gatctgcttc
     5341 atgcgtttct taccttcttt ctgcttctgc agcagctttt tcttacggct gatatcgccg
     5401 ccataacatt tagccagtac gtttttacgc agctgtttca cggtggatcg cgcaatgatg
     5461 tgcgtaccaa tcgctgcctg aatggcgata tcaaactgct ggcgtgggat cagatctttc
     5521 atcttctcca ccaactcgcg accgcggttt tgcgaattat cacggtgggt gatcaacgcc
     5581 agcgcatcaa cacgttcacc gttgattaat acgtctacac gtaccatgtc ggacgcctgg
     5641 aagcgcttga agttgtaatc cagagacgca taaccacgcg aggtagattt caggcgatcg
     5701 aagaagtcga gcaccacttc cgccatcggg atctcgtacg tcagcgccac ctgattaccg
     5761 tggtaaacca tattggtctg cacgccgcgt ttttctacgc acaacgtaat aacgttgccg
     5821 agatatgcct gcggcagcag catgtgacac tctgcaatcg gctcgcgcag ttcgtagatg
     5881 ttatttaccg caggcagctt ggatgggctg tcgacgtaga taacttctct tgacgtggtt
     5941 tcaacttcat acactacggt cggcgcagtg gtgatcagat ccagatcgta ttcacgttcc
     6001 agacgttcct ggatgatctc catgtgcagc aggccgagga agccgcagcg gaaaccaaag
     6061 cccagcgcgc tggagctttc cggctcatag aacagtgagg catcgttcag gctgagttta
     6121 cccagcgcgt cacggaaggc ttcatagtcg tcggaactta ccgggaacag accggcgtat
     6181 acctgcggtt tgactttctt aaagccaggc agcgcctttt ctgccggatt acgcgccagc
     6241 gttaaggtat cgccgactgg agcgccgtgg atatctttaa tcgcacatac gagccagcct
     6301 acttcgccac atttcagttc agtgcggtca acctgtttcg gcgtgaagat gcccagacgg
     6361 tcggcgttat aggtctgccc ggtactcatg actttcactt tgtcgccctt acgcagggtg
     6421 ccgtttttaa tacggataag tgaaacaacg cccaggtagt tgtcgaacca tgagtcgata
     6481 attagtgcct gcaacgggcc ttccggatcg ccttccggcg gcggaatgtc gcgcaccaga
     6541 cgttcgagaa cgtcctgcat accaacgccg gttttcgctg aacagcgcac cgcgtcggtg
     6601 gcgtcgatgc cgacgatatc ttcaatttct tccgccacgc gttcaggatc ggctgccggc
     6661 aggtcaatct tgttcagtac cggcacaact tcgagatcca tttccatggc ggtgtagcag
     6721 tttgccaggg tttgcgcttc tacgccctgc ccggcgtcga ccaccagcaa tgcaccttca
     6781 caggcagcca gcgaacggga aacttcatag gagaagtcta cgtggcccgg ggtgtcgata
     6841 aagttaagct gataggtttc gccgtcagac gctttgtagt ccagcgtcac gctttgcgct
     6901 ttgatggtaa tgccacgctc acgctcaaga tccatggaat cgagaacctg cgcctccatt
     6961 tcacggtcag acaggccacc gcagatctgg ataatacggt cagacagcgt cgatttaccg
     7021 tggtcaatgt gagctatgat cgaaaagtta cgtatattct tcataaagta tgattattgt
     7081 gccttaatgc ccggttaacc aggcttttag aagtcgctgt tctgagctta acgtctgtat
     7141 taatagaaac gccgcattct acactacaac attgaggcga ggaaatgttc ataccgtatg
     7201 gattgtggta tctggaaacg tcctcgcatt tgttatgcaa aatgcaacaa agccagtgaa
     7261 atcactggct cgcgtcttcc gaagatgttt caaatcgcac aaggccaggc ggcaatgcca
     7321 cgcttaagat gatcggttgc cattctgccc gggcagcaaa cttacgcgag tagccgcgcg
     7381 caatcaggaa cccgccaata ccaccgagga tcgcaccaca taatgctgcg acgtcagaag
     7441 caaagagtag ctgaaatagc gaagcgataa ggaataatcc caccagcggc gacatataaa
     7501 ccagtaatgc ggagctaagc aggctgcctt cggcgatccc caattccact ttttgccccg
     7561 gcactaacgg ctcatcacag ggtacgacaa tggtatgcgt ggtttgcggg ccaagtttat
     7621 ttaacacgcg gctaccgcaa ccggcgcgtg aagcgcagct gctgcatgaa gctttaacat
     7681 cacaactgac cagcgcctgc ccgttttgcc aggagacgac ggtagcccac tctttgatca
     7741 ttgcgctgcc ccgaacttaa tattctcggc aatgcgtttc gccgtttgcg gcggcagttc
     7801 accgacaatg gtgatttcgg cgttatcacg tacgcttgta ctgacggttc tgcgtccggt
     7861 gcgcaacatc tgatcggtgc tcgatggcgt agcgcggtta acgtttaccg agaagctgaa
     7921 taatccgtcg gaatagagac gtgattcgat aggcatgttg tccatcgtcg gtagcggacg
     7981 tcgactactg gaaacttcgc taaaaccctg tggcaaccag gttggcgtcc agctgaattt
     8041 agctttttca cctacaggaa cagaaagcaa cggcggcaaa tttgccttcg ccagcgtctg
     8101 catactgctg ctgatatcct gattgacgtt aaaagcaatc acgcgaaatt gttccagcgt
     8161 ttcaccatcg cgatcaagga gatcaacccg catcggtaat ttcgattcgg tgtccatcca
     8221 cacgatgtag ctgtagcgtg taccatctcg ggcaaccacg cgaatgactt cgcaaagacg
     8281 atcagcaata cgcgtgcgcc cgacggagat aaagtcgtag taaggagaaa ggcgtttgaa
     8341 atcggtatag ataagcgatg gcagagaatc aacaatgtaa tcgccattaa gcgtgaacgg
     8401 ttcaagtccc ggttcaaaat agctgatttc attgccgcgc tgtaccactt cccggcgcgg
     8461 gccatccatt tgcaacaatt gtgcaagagg acggttatcg aggcgtgcat gtcgataacg
     8521 cagagactca acaccctgtt tattgatgct gatgaatgac agctcgtaat tcagtgactg
     8581 actggccagg ttcatctgct gtaataacgc cccggacgcg ggagtggccg aggcgttagc
     8641 agagaataac aggctacctg tcactaatga catggcaaac caaagttgct tcattactgc
     8701 gattgcgttc ctaaagtttg aattcctggc acctgtacag cggcttgctg ggtttgcgcc
     8761 tgctcaaact gaagctgttc agagtggagt cggcgttgca gttcgtaatc ctgcaacatt
     8821 gcattaatgc gacgacgctg ctcctgtacc tgctgctgtt gaccattgtt tgcggtcgct
     8881 tcagaaggta ctcccaggct taccgggctg gctttaccca tcatcggcag tgtattaaat
     8941 accggcgttt cgggctgctg ggacgtttca gattgtccat tatagtgctg gacgccaacg
     9001 ataactgcaa gcgatacgca tgcggctacg cccatttggg taagctgtgc cgcccacgga
     9061 cgtactttct gccagaatgg cattttctgc cattgatgcg gcgcaggctg ggcttccggg
     9121 atcaatgtcg ccggttgacg tactggctct tcttcaatgg cggccatcac gcgtgaagag
     9181 atatcgaaat ggagcacctc gggagtatca ccccgcattg agtcacggat taagtgatag
     9241 ctttcccagg ttttctgcat ttctgggtta tgagccagtt cgttaagcag ctcactatcc
     9301 agcgtttcgc catccattaa agcggaaagt tgttctttct gcatgcctaa tacccttatc
     9361 cagtatcccg ctatcgtcaa cgcctgataa gcggttgaac tttgttatca atagcttccc
     9421 tcgctcggaa gatacgtgaa cgcaccgtac ctaccggaca atccatgata gcggctatct
     9481 cttcatagct caggccatcc agctcccgca aggttattgc catgcgtaaa tcttccggga
     9541 gggactcaat agttcggaaa actatctgtc tcagttcttc tgacaacatt aagttctcag
     9601 ggttcgaaat ttctttcaac gcgccgccac tttcgaagtt ttcagcttca atggcatcca
     9661 catcactgga aggtggacga cgcccctgag caaccaggta atttttcgct gtatttacag
     9721 caatccgata cagccatgta taaaaagcgc tatctccccg gaacgaatcc agcgcacgat
     9781 aggctttaat aaaagcttct tgtaccacat cgggaacatc acccgacggc acatagcggg
     9841 aaaccagact cgccacttta tgctgatagc gcactaccag taagttaaag gctttctgat
     9901 ctcccttctg gacccgttca accaggacct ggtccgttaa ctgctcgctc atccgaggta
     9961 aagtctcccc aaaccaaatt tccacgcgct atcgaaacgc cactccatta gctgcaattt
    10021 gagcaagcaa aggattagag tgtctcgttt ttgtaaagtt ccgtaacgca tctgtttttg
    10081 tttgtcatgc tgtagacgga tcattatcta tcattataag tctacagaat ctgaacatcg
    10141 cattatctgt gtagaaatgc ccatttaact gcctgaagag taacccaacg gcctttttat
    10201 ttcaccacct aatcctccac cagccagtaa cttctctttt tctcgccgcc ctgcatcagc
    10261 gtgtttagcc actgtaacaa atattaaaat agcaggtgtt tattcgcaca atatgatgct
    10321 atgctgacca aaccgtgttt agtaaattaa acaaagaaaa tgaatactct ccctgaacat
    10381 tcatgtgacg tgttgattat cggtagcggc gcagccgggc tttcactggc gctacgcctg
    10441 gctgaccagc atcaggtcat cgttctaagt aaaggcccgg taacggaagg ttcaacattt
    10501 tatgcccagg gcggtattgc cgccgtgttt gatgaaactg acagcattga ctcgcatgtg
    10561 gaagacacat tgattgccgg ggctggtatt tgcgatcgcc atgcagttga atttgtcgcc
    10621 agcaatgcac gatcctgtgt gcaatggcta atcgaccagg gggtgttatt tgatacccac
    10681 attcagccga atggcgaaga aagctaccat ctgacccgtg aaggtggaca tagtcaccgt
    10741 cgtattcttc atgccgccga cgcaactggt agagaagtag aaaccacgct ggtgagcaag
    10801 gcgctgaacc atccgaatat tcgcgtgctg gagcgcagca acgcggttga tctgattatt
    10861 tctgacaaaa ttggcctgcc gggcacgcga cgggttgttg gcgcgtgggt atggaaccgt
    10921 aataaagaaa cggtggaaac ctgccacgca aaagcggtgg tgctggcaac tggcggtgcg
    10981 tcgaaggttt atcagtacac caccaatccg gatatttctt ctggcgatgg cattgctatg
    11041 gcgtggcgcg caggctgccg ggttgccaat ctcgaattta atcagttcca ccctaccgcg
    11101 ctatatcacc cacaggcacg caatttcctg ttaacagaag cactgcgcgg cgaaggcgct
    11161 tatctcaagc gcccggatgg cacgcgtttt atgcccgatt ttgatgtgcg cggcgaactg
    11221 gccccgcgcg atattgtcgc ccgcgccatt gaccatgaaa tgaaacgcct cggcgcggat
    11281 tgtatgttcc ttgatatcag ccataagccc gctgatttta ttcgccagca tttcccgatg
    11341 atttatgaaa aattgctcgg gctggggatt gatctcacac aagaaccggt accgattgtg
    11401 cctgctgcac actatacctg cggtggtgta atggttgatg atcatgggcg tacggacgtc
    11461 gagggcttgt atgccattgg cgaggtgagt tataccggct tacacggcgc taaccgcatg
    11521 gcctcgaatt cattgctgga gtgtctggtc tatggctggt cggcggcgga agatatcacc
    11581 agacgtatgc cttatgccca cgacatcagt acgttaccgc cgtgggatga aagccgcgtt
    11641 gagaaccctg acgaacgggt agtaattcag cataactggc acgagctacg tctgtttatg
    11701 tgggattacg ttggcattgt gcgcacaacg aagcgcctgg aacgcgccct gcggcggata
    11761 accatgctcc aacaagaaat agacgaatat tacgcccatt tccgcgtctc aaataatttg
    11821 ctggagctgc gtaatctggt acaggttgcc gagttgattg ttcgctgtgc gatgatgcgc
    11881 aaggagagtc gtggcctgca ttttacgctg gattacccgg aactgctcac gcattccggt
    11941 ccgtcgatcc tttcccccgg caatcattac ataaacagat aaaaagcctg ggtcagcgcc
    12001 gtatacgctt cggaatagtt ctggtctggc ccacgaatga ctaagcgatc gctaaagcat
    12061 tctcccgcct gcggggagaa tgccagcagc acccgatgcg gcagtcgcgc ttcgttttcc
    12121 gccacatccg tccgcaaacg taaatgccag cccatgctta atgccagctc cgtaaaacca
    12181 ttaccaatct gctctggcag cactacgcag aaaaatccct cttcggtaat gcactccgcc
    12241 gcacaggtca gcaacgatgg gtgatcaagc gtagtggtat agcgagcctg ttcccgttga
    12301 ggtgtcgagc actctactcc ctgctgatag taaggtgggt tactgatgat taaatcgaag
    12361 cgtactgtct gctgtgtgat ccactgctga atatccgccg tatggacgtt aatccgctct
    12421 gcccacgggg actggttgat attttcctgc gcctgcgcgg cagcttcact ttccagttca
    12481 actgcatcaa tcatcacgct gtcatcggtt cgctgcgcca gcattaatgc cagcaacccg
    12541 ctacccgcgc cgatatcaag gcaacgtttt accccagcca ccggtgccca tgcgcccaat
    12601 aaaataccat ccgttcccac tttcatcgca cagcgatcgt gagcaacaaa aaactgttta
    12661 aaagtaaatc cattacgacg aagcacggat gtagactgtg acatgaaaat aaaaccttgc
    12721 aggaaaaacg gcgatagcac cgggtgagaa caatacccga gaagcgatat ccatacaaac
    12781 agatgaagat tgcagccgta acgtctataa tcagcgcccc acacagaggt agaacatgac
    12841 tgtaacgact ttttccgaac ttgaactcga cgaaagcctg ctggaagccc tccaggataa
    12901 aggtttcact cgcccgaccg ccattcaggc tgccgccatt ccgcctgcgc tcgatggccg
    12961 tgatgtactc ggttctgcgc cgacaggcac cggtaaaacg gcggcgtatc tgctgccagc
    13021 gttgcagcac ctgctcgatt tcccgcgtaa gaaatccggt ccgccgcgta ttttgatcct
    13081 caccccaact cgcgagctgg cgatgcaggt ggccgatcat gcccgcgaac tggcgaaaca
    13141 tacgcatctg gatatcgcca ccatcaccgg cggcgtagcc tatatgaacc acgcggaagt
    13201 gttcagcgaa aatcaggaca tcgtggtcgc cacgaccgga cgtctgctgc aatacataaa
    13261 agaagagaac ttcgattgcc gcgcggttga aacgctgatc ctcgacgaag cagaccgtat
    13321 gctggatatg ggcttcgctc aggatatcga acatattgct ggcgaaacgc gctggcgtaa
    13381 acagaccctg ctcttttcgg caacgctgga aggcgatgcg attcaggact ttgccgagcg
    13441 tctgctggaa gatccggtgg aagtttctgc caatccctcc acccgtgagc gcaaaaaaat
    13501 tcatcagtgg tattaccgcg ccgatgatct tgagcataaa accgcgttgc tggtgcatct
    13561 gttaaaacag ccggaagcga cccgctcaat tgtgtttgtg cgtaagcgtg agcgtgtgca
    13621 tgagctggca aactggctgc gcgaagcggg catcaacaac tgctatctcg aaggtgagat
    13681 ggtacagggc aagcgtaacg aagcgatcaa gcgtttgacc gaaggtcgcg taaacgtact
    13741 ggtcgcaacc gatgttgccg cgcgcggtat cgacattcct gacgtcagcc acgtctttaa
    13801 cttcgatatg ccgcgcagtg gcgatactta tttgcaccgt atcggacgta ccgcgcgcgc
    13861 cggtcgtaaa ggcaccgcaa tttcgctggt ggaagcccat gaccatctgc tgctgggtaa
    13921 agtaggccgc tatattgaag agccaattaa agctcgcgtt attgatgagt tacgcccgaa
    13981 aacgcgtgcg ccaagcgaaa agcagaccgg caagccatcg aagaaagtac tggctaaacg
    14041 tgctgagaag aaaaaagcta aagagaaaga gaagccgcgg gtgaaaaaac gccatcgcga
    14101 caccaaaaat attggtaagc gccgtaaacc aagcggaacg ggcgtgccac cgcaaacgac
    14161 agaagagtaa tctcaatgcc aggtttaagc ctggtattaa aagtgctggc aaacgcaaaa
    14221 ctgcctgatg cgctacgctt atcaggccta cgtggctcat gcaatatatt gaatttgcac
    14281 gatcttgtag gccggataag gcgttcacgc cgcatccggc atgaataaag cgcactttgt
    14341 cgacaagtta aatgccgggt ttaatcccga cattattttt atccagccac aaaactctct
    14401 tcaacacact gaataaaagt gtgcatcgcc gggctaaccg cttttccagc atgatgtgcg
    14461 cacatggccg taatggtctg cgactgttcg ccaaaaggca attcaattaa ttctccgcac
    14521 tccaactctt ttgccaccgc aaagcgcggt aggtagctga ccccgatatt cgccgcgaca
    14581 caacgcttga tgctttcgat acttataagc tcaatggtgt tttccaccgt gatccgccgc
    14641 tgacgcagcg tgctctcaaa tatctgccgg aagacacatt gcggttcgtt tataataaag
    14701 ctacaggcgt tatgtcttcc cggctcagta aaatcgacat ctgcaatttg cggtgaagcc
    14761 accagcacca gtgattgttc acccaactct cgtcgattca gagcatcatc attccctaca
    14821 cgataaaaga cgccaacatc ggcctcatca ttcagtagca catcacggat cacgtaacag
    14881 ttcagcgact gcaacgataa acgcactttt ggggcgcgtt gccgaaaacg ctgcaacacc
    14941 tgcggcattc ggtaagagag caacgtttcg cccgaaacaa cgcgtagttc cccgtccgga
    15001 tctgactcct ttttggcagc ctcacgaagc gtatccatca ctcgggtaag ttcataaatg
    15061 tgcggcagca acttttttcc ttcgcgggta aggcacatcc gtcggccaat tttctcaaat
    15121 aactggactg aaaactcctg ctcaagctgc tgaatatgaa aagtcaccgt cgattgtgta
    15181 cagcacaatt tttgcgaagc tcgcaaaaag gaaccctctt ccaccacggt tttaagcgta
    15241 ataaaacggc gcagatccat aaccccaaac ctatcgaaaa tatcgaatct agaatataaa
    15301 aacattcatt tttttaaatg ttccgtgtcg ggtactgtct accaaaacag aggagataac
    15361 aagtgacacc gaccctttta agtgcttttt ggacttacac cctgattacc gctatgacgc
    15421 caggaccgaa caatattctc gcccttagct ctgctacgtc gcatggattt cgtcaaagta
    15481 cccgcgtgct ggcagggatg agtctgggat ttttgattgt gatgttactg tgtgcgggca
    15541 tttcattttc actggcagtg attgacccgg cagcggtaca ccttttgagt tgggcggggg
    15601 cggcatatat tgtctggctg gcgtggaaaa tcgccaccag cccaacaaag gaagacggac
    15661 ttcaggcaaa accaatcagc ttttgggcca gctttgcttt gcagtttgtg aacgtcaaaa
    15721 tcattttgta cggtgttacg gcactgtcga cgtttgttct gccgcaaaca caggcgttaa
    15781 gctgggtagt tggcgtcagc gttttgctgg cgatgattgg gacgtttggc aatgtgtgct
    15841 gggcgctggc ggggcatctg tttcagcgat tgtttcgcca gtatggtcgc cagttaaata
    15901 tcgtgcttgc cctgttgctg gtctattgcg cggtacgcat tttctattaa cgaaaaaaag
    15961 cggaagaggt cgccctcttc cgcttagtaa cttgctactt aagccttaca ggctttcagt
    16021 aaaggtacga gcgataacgt cgcgctgctg ttccggagtc agagagttaa agcgaactgc
    16081 ataaccggat acacggatgg tcagctgcgg atatttttcc ggatgcttaa ctgcatcttc
    16141 cagagtttca cgacgcagaa cgttaacgtt caggtgttga ccaccttcaa cgcgaacttc
    16201 tggtttcact tctactggaa cttcacggta ttcaatgtca cccagtttgc ttactgcaac
    16261 cacttcatct tctgcataac ctgcttttgc aacgatgcaa cgtgcttcgc ctttttcgct
    16321 gtccagcagc cagaaagagt tcagcagatc gtcgttagcg gctttagtaa tctggatacc
    16381 tgtaatcatg tgatgcctcc ccggcaaaat tatttgattt gttcagcctg tcgcggccaa
    16441 ttggtaaaac cattgttgct tgagtgtata tatactcctc aaccaccctt gattctttga
    16501 tttaaatcaa taaaaaccac acatcgattg tggtcgtaaa tggattttat tgttttacat
    16561 caacttatgc gggtgtgaaa ttttaccaat ttacattttt ttgcactcgt ttaagtctaa
    16621 aaaatgagca tgattttgtt ctgtagaaag aagcagttaa gctaggcgga ttgaagattc
    16681 gcaggagagc gagatggcta acgaattaac ctggcatgac gtgctggctg aagagaagca
    16741 acaaccttat tttcttaata cccttcagac cgtcgccagc gagcggcagt ccggcgtcac
    16801 tatctatcca ccacaaaaag atgtctttaa cgcgttccgc tttaccgagt tgggtgacgt
    16861 taaagtggtg attctcggcc aggatcctta tcacggaccg ggacaggcgc atggtctggc
    16921 attttccgtt cgtcccggca ttgccattcc tccgtcatta ttgaatatgt ataaagagct
    16981 ggaaaatact attccgggct tcacccgccc taatcatggc tatctcgaaa gctgggcgcg
    17041 tcagggcgtt ctgctgctca atactgtgtt gacggttcgc gcaggtcagg cgcattccca
    17101 cgccagcctc ggttgggaga cgtttactga taaagtcatc agcctgatta accagcaccg
    17161 cgaaggcgtg gtgtttttgt tgtggggatc acatgcgcaa aagaaagggg cgattataga
    17221 taagcaacgc catcatgtac tgaaagcacc gcatccgtcg ccgctttcgg cgcatcgtgg
    17281 attctttgga tgcaaccatt ttgtgctggc aaatcaatgg ctggaacaac gtggcgagac
    17341 gccgattgac tggatgccag tattaccggc agagagtgag taaatttgcg gggaaatgcc
    17401 ggatggcaga gttgccaccc ggctgattta tcaggcttta ttctgacgcc accattcacc
    17461 aagcaaaacg ccggttgcga cagagatatt cagcccggca acgttgcccg taccgtcaat
    17521 cttcacgcgc agatcgttcg gatcgcgtgc ggcatccggt aacccttcat attcctgacc
    17581 cagcaccagt accattttcg ctggcagact agttttgaac agcggtttac cctgctcgct
    17641 ggaagtggtc actacggtgt aacctgcctg acggaaatca tccagcacgt taacaatgtt
    17701 gtcgccagta atcggctgaa cgtgctctgc gccgccttct gcggtacgga tagccgcccc
    17761 cgactccagc agtgccgcat cctgcaccac gacacctttc acgccgaagt gcgcgcagct
    17821 gcgcatcatg ccacccaggt tatgcgggtt agattcgttt tccagtgcca gaacgcaatc
    17881 ctgcgcgcct gcctggctta cccactgctg cacggttgta ccgttacgct ttttgatcaa
    17941 gaagcaaacg ccgccgtgat gttccgtgcc tgacgctttt gtcaattccg cttcatccac
    18001 cacatggtac gctttgcggt ttgctgccat ccagcgcaaa gcttctttaa aacgcggcgt
    18061 tacactctgg ataaaccagg cgcgaacaat cgcttccgga cggctctgga aaagtgcctg
    18121 acatgcattt tcgccgtaga cgcgggtttc ttccgcacgc tgacgacgca acacttccgg
    18181 atcaataaaa cttttaccac tgatgccacc gtgatcggcc ttttccggcg tctcatcacc
    18241 cggcgcgcgg gaaaccgtgc gccacggcga atcttcccac ttgcggtcac ggggctgact
    18301 ctgtttgtca tcgcgggcgg ggcgacggcc accgtcagca cgagattttc ctggacgccc
    18361 gccccctttc ccggtacgcg ggttgtgggt acgtttatca gaatcatcat cactgcggac
    18421 atacatcact ttgaccttgc cgcttttacc tttcatttca tcgttcatgc ttttctccac
    18481 cagcgctgcg cgaagcgcgc agattacccg aagtccgcgc ggttcgccat gatttcgtac
    18541 caaagcctgc gactatcata cctattgaat aaaacagatt gttgtctgga acaatgtccc
    18601 cgataatatg taacatatta gaaacatacc ggcgtcgttg ccgataagtc tccttactca
    18661 tcccgaggtt agttatgaat accgtttgta cccattgtca ggccatcaat cgcattcccg
    18721 acgatcggat cgaagatgcg gcaaaatgcg gacgctgcgg tcacgacttg tttgacggag
    18781 aggtgattaa tgcgaccggt gaaacactcg acaaattgct gaaggatgat ctacctgtgg
    18841 tgatcgactt ctgggcaccg tggtgcggcc cctgccgtaa tttcgcacca atttttgaag
    18901 atgtcgcgca agagcgtagc ggtaaagtgc gctttgtgaa agtgaatacc gaagctgaac
    18961 gtgaattgag cagtcgcttt ggaattcgta gtataccgac gatcatgatt ttcaaaaacg
    19021 gtcaggttgt cgacatgctt aatggcgcag taccgaaagc gccgttcgat agctggctga
    19081 acgaatctct ttaatcttac cggggcgcat cttgtgcccc gttttctcct ctgcgacaat
    19141 ggcgtttttt cgacgctctc ttatgaccga aaacgctgtt ctccagttac gcgccgagcg
    19201 tattgcgcgc gcaacacgtc cttttcttgc ccgcggtaat cgcgttcgtc gctgccaacg
    19261 ctgtcttttg ccagagaaat tatgtctctg ttcgacaatt accccagcac aagcaaaaag
    19321 tcgcttctgt ttgctgatgt tcgacaccga gccaatgaag cccagtaata ccgggcgtct
    19381 cattgctgat attttgcctg ataccgttgc gtttcaatgg tcgcgtaccg aaccctcgca
    19441 ggatttgctg gagttggtgc aaaacccgga ctatcagcca atggtggtct ttcccgcttc
    19501 gtatgctgat gagcaacggg aagtgatctt cacaccacct gccggtaagc caccgctgtt
    19561 tatcatgctc gatggtacct ggccggaagc tcgcaagatg tttcgtaaaa gtccgtatct
    19621 ggataatctt cccgtcattt ccgtcgatct ttcccggctt tctgcctatc gcctgcgtga
    19681 agcccaggct gaaggccaat attgtactgc cgaggtagcc atcgcactgt tagatatggc
    19741 cggcgatacc ggggcggcgg cagggttagg cgagcatttt acccgcttta aaacacgcta
    19801 tctggcagga aaaacgcaac atctgggtag catcacagca gaacagttag aaagcgttta
    19861 aaatcattcg gtcacttctg cgggagaccg gtatgagtca gcgaggactg gaagcactac
    19921 tgcgaccaaa atcgatagcg gtaattggcg cgtcgatgaa acccaatcgc gcaggttacc
    19981 tgatgatgcg taacctgctg gcgggaggct ttaacggacc ggtactcccg gtgacgccag
    20041 cctggaaagc ggtgttgggt gtgttggcct ggccggatat tgccagcttg ccctttacac
    20101 ccgaccttgc ggttttatgt accaatgcca gccgtaatct tgctcttctg gaagagctcg
    20161 gcgagaaagg ctgtaaaacc tgcattattc tttccgcccc ggcatcgcaa cacgaagatc
    20221 tccgcgcctg cgccctgcgc cataacatgc gcctgcttgg accaaacagt ctgggtttac
    20281 tggctccctg gcaaggtctg aatgccagct tttcgcctgt gccgattaaa cgcggcaagc
    20341 tggcgtttat ttcgcaatcg gctgccgtct ccaacaccat cctcgactgg gcgcaacagc
    20401 gtaagatggg cttttcctac tttattgcgc tcggcgacag cctggatatc gacgttgatg
    20461 aattgcttga ctatctggca cgcgacagta aaaccagcgc catcctgctc tatctcgaac
    20521 agttaagcga cgcgcgacgc tttgtttcgg cggcccgtag tgcctcgcgt aataaaccga
    20581 ttctggtgat taaaagcgga cgtagcccgg cggcacagcg actgctcaac acgacggcag
    20641 gaatggaccc ggcatgggat gcggctattc agcgtgccgg tttgttgcgg gtacaggaca
    20701 cccacgagct gttttcggcg gtggaaaccc ttagccatat gcgcccgcta cgtggcgacc
    20761 ggctgatgat tatcagcaac ggtgctgcgc ctgccgcgct ggcgctggat gccttatggt
    20821 cacgcaatgg caagctggca acgctaagcg aagaaacctg ccagaaactg cgcgatgcac
    20881 tgccagaaca tgtggcaata tctaacccgc tcgatctacg cgatgacgcc agcagtgagc
    20941 actatattaa aacgctggat attctgctcc acagccagga ttttgacgcg ctgatggtta
    21001 ttcattcgcc cagcgccgct gctcccgcaa cagaaagcgc gcaagtatta attgaagcgg
    21061 taaagcatca tccccgcagc aaatatgtct ctttgctgac gaactggtgc ggcgagcact
    21121 cctcgcaaga ggcacgacgt ttattcagcg aagccgggct gccgacctac cgtaccccgg
    21181 aaggaaccat cactgctttt atgcatatgg tggagtaccg gcgtaatcag aagcaactac
    21241 gcgaaacgcc ggcgttgccc agcaatctga cttccaatac cgcagaagcg catcttctgt
    21301 tgcaacaggc gattgccgaa ggggctacgt cgctcgatac ccatgaagtt cagcccatcc
    21361 tgcaagcgta tggcatgaac acgctcccta cctggattgc cagcgatagc accgaagcgg
    21421 tgcatattgc cgaacagatt ggttatccgg tggcgctgaa attgcgttcg ccggatattc
    21481 cacataaatc ggaagttcag ggcgtcatgc tttacctgcg tacagccaat gaagtccagc
    21541 aagcggcgaa cgctattttc gatcgcgtaa aaatggcctg gccacaggcg cgggtccacg
    21601 gcctgttggt gcaaagtatg gctaaccgtg ctggcgctca ggagttgcgg gttgtggttg
    21661 agcacgatcc ggttttcggg ccgttgatca tgctgggtga aggcggtgtg gagtggcgtc
    21721 ctgaagatca agccgtcgtc gcactgccgc cgctgaacat gaacctggcc cgctatctgg
    21781 ttattcaggg gatcaaaagt aaaaagattc gtgcgcgcag tgcgctacgc ccattggatg
    21841 ttgcaggctt gagccagctt ctggtgcagg tttccaactt gattgtcgat tgcccggaaa
    21901 ttcagcgtct ggatattcat cctttgctgg cttctggcag tgaatttacc gcgctggatg
    21961 tcacgctgga tatctcgccg tttgaaggcg ataacgagag tcggctggca gtgcgccctt
    22021 atccgcatca gctggaagaa tgggtagaat tgaaaaacgg tgaacgctgc ttgttccgcc
    22081 cgattttgcc agaagatgag ccacaacttc agcaattcat ttcgcgagtc accaaagaag
    22141 atctttatta ccgctacttt agcgagatca acgaatttac ccatgaagat ttagccaaca
    22201 tgacacagat cgactacgat cgggaaatgg cgtttgtagc ggtacgacgt attgatcaaa
    22261 cggaagagat cctcggcgtc acgcgtgcga tttccgatcc tgataacatc gatgccgaat
    22321 ttgctgtact ggttcgctcg gatctcaaag ggttaggctt aggtcgacgc ttaatggaaa
    22381 agttgattac ctatacgcga gatcacggac tacaacgtct gaatggtatt acgatgccaa
    22441 acaatcgtgg catggtggcg ctagcccgca agctcgggtt taacgttgat atccagctcg
    22501 aagaggggat cgttgggctt acgctaaatc ttgcccagcg cgaggaatca tgagtaaggt
    22561 actggaaatg ttgaccactt aatcgggact ggtgttatta ttgcccgctt atgtcgtctg
    22621 cattgcacag aggacccttc aatgaacaga gaagaaatgc actgtgatgt tgtcaaaatt
    22681 taagcgtaat aaacatcaac aacaccttgc ccaactaccc aagatttctc aatcagttga
    22741 tgatgtcgat ttcttttacg ctcccgccga cttccgggag acgctgctgg aaaaaatagc
    22801 cagcgcgaag cagcgcattt gcattgtcgc cctgtatctc gaacaggatg acggtggcaa
    22861 aggcattctg aacgcgttgt atgaagctaa aaggcagcgt ccggaactgg atgtgcgggt
    22921 gctggtcgac tggcatcgtg cacaacgtgg acgcattggc gctgcggcat ctaacactaa
    22981 cgctgactgg tactgccgca tggcgcagga aaatccgggc gtagatgttc cggtttatgg
    23041 cgttccaatc aatacccgtg aagcccttgg tgttctgcac tttaaaggct ttatcatcga
    23101 cgatagcgta ctttatagcg gtgccagcct gaacgatgtt tacctgcatc agcacgataa
    23161 atatcgctac gaccgttatc atcttatccg taaccgtaag atgtcagaca ttatgtttga
    23221 atgggttaca cagaatatta tgaatggccg cggcgttaat cgtctggatg atgttaatcg
    23281 gccaaaaagc ccggaaatca agaacgatat tcgtctgttc cgccaggagc tgcgtgatgc
    23341 cgcttatcat ttccagggcg atgccgacaa cgatcagctt tccgtaacgc cgctagtggg
    23401 gctggggaaa tcgagtctgt tgaacaagac cattttccat cttatgcctt gtgccgagca
    23461 gaaactaacc atctgtacgc catacttcaa cctgccagca atccttgtgc gcaatattat
    23521 ccagttgctg cgcgaaggga aaaaggtcga aattattgtt ggtgataaaa ccgcgaacga
    23581 cttctacatt ccggaagatg aacctttcaa gataattggc gcattgcctt atctctatga
    23641 gatcaatctg cgtcgtttcc tgagccgttt gcagtattac gtcaatactg accagttagt
    23701 ggttcggtta tggaaagatg acgacaacac ctatcacctg aaagggatgt gggttgatga
    23761 taagtggatg ttgatcaccg gtaataacct gaacccgcgc gcctggcgtc tggatctgga
    23821 aaacgccatt ttgatccacg atccacaact ggagcttgcg ccgcagcgag agaaagaact
    23881 ggagctgatc cgcgagcata ccaccatcgt taagcactat cgcgatctgc aaagtattgc
    23941 cgattatccg gtgaaggttc gtaaactcat ccgccgtttg cgccgtatcc gcatcgaccg
    24001 attaattagc cgaatcctgt aatcacaacc ccgtcctgta cggggtttgt tttttggagg
    24061 ccacgttttg cgtattcttt ttgtctgttc gctattgtta ctttctggat gcagccatat
    24121 ggctaacgat agctggagcg ggcaggataa agctcaacac tttatcgcct cggcgatgct
    24181 ttccgccgcc ggaaatgaat attcacagca tcaggggatg agccgggatc acagtgccat
    24241 gtttggattg atgttctctg tcagtttggg ggcgtcaaaa gagctttggg atagccgccc
    24301 cgaagggagc ggctggagct ggaaggattt ggcctgggat gtcgccggtg caagcaccgg
    24361 ctataccgtc tggcaactga cccgtcacta aagacgcatc cccttccctt tgcgatgtag
    24421 catcaaagaa accagaaacg ccaccacggc catcaaggtc acataccaga agaaggctgt
    24481 ttccattcct attgatttca gcgacaacgc tacgtactcc gccgaaccac caaatatagc
    24541 attagcgacc gcatatgaca gaccaacgcc taatgcgcga acctgtgccg ggaacatctc
    24601 agccttcagt attccactga ttgatgtata aaaactcact atcagcaggg cacacatcac
    24661 cagaccaaaa gcggcataag gcgaggaaac gttttgcaat gctgagagaa taggaacggt
    24721 aaaaatggct gccagcgaac cgaaacataa cattgaggta cggcgaccaa tcttatccga
    24781 cagcgcgcca atgagtggtt gaataagcat gaatacaaac aatgcggcag tcataatgcc
    24841 actcgccacg ttggcatgca ttcccgcagt atttaccaga tacttctgca tataagtagt
    24901 gaaggtatag aaacaaaggg agcccgcagc ggtaaaaccg agaaccatga tgaatgcacg
    24961 gcgattgcgc cataatcctt tcagagatcc agcttctttt aaagcgcgcg tttcttgttg
    25021 cgaagtttca tctaactgac gacgtaacca caacgccaca acagctaaca cagctcctaa
    25081 cgcgaaagga atacgccatc cccactctct gagtgcagcg tcttccatgg tgtgttgtaa
    25141 aaccacgaca accagtaggg ctagcagttg tccgccgatc aacgtcacat actgaaatga
    25201 tgcgtaaaaa cctttgcgcc cttcaacggc aacttcactc atataggtgg cgctggtgcc
    25261 atattctccg ccaacggata atccctgaaa taaacgagcg agaagcaata atgccggagc
    25321 ccacgtacct atagtttcat aacctgggag gcaggcgata accagcgatc cgaaacacat
    25381 catacacacc gataacagca tcgatttttt gcgaccatgt ttatcggcta tgcggccaaa
    25441 tagccaaccg cctattgggc gcatcaggaa tcccgcagca aaaacacctg ctgtttgtag
    25501 tagttgagtc gtcgtgttcc cggaagggaa gaaaatgtgg gcgaagtaga gtgaacagaa
    25561 cgagtagaca tagaaatcga accactcgac cagattacct gaagaggccc ccacaatcgc
    25621 ccaaatgcgg cgacgagtat cactacttgt cagtttgctg tctgccgtta cagtactttc
    25681 agccatgcca ttatgtctcc tgccgtaatc cgatgctttt gttggtcgct tttgtttatt
    25741 tcttttggta aagaaaatgt tatacatttg ttgcgtatca ttatgcaacc ttaaccatga
    25801 atttagttag caggaaaatg gttattgagg agcttaagga taaatttctg gtaaggagga
    25861 cacgtatgga agtaggcaag ttggggaaac cgcatccgtt gctgaatctg gcatatgtgg
    25921 gagtataaga cgcgcagcgt cgcatcaggc atttttttca ccaacgcaaa aaggccatcc
    25981 tgacggatgg cctttcgctt
//
LOCUS       DAPHBJ010000051        25407 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.051, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000051 DAPHBJ010000000
VERSION     DAPHBJ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.051"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(146..1027)
                     /gene="prmA"
                     /locus_tag="RHZ76_001865"
     CDS             complement(146..1027)
                     /gene="prmA"
                     /locus_tag="RHZ76_001865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDV2812517.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(1039..2490)
                     /gene="panF"
                     /locus_tag="RHZ76_001866"
     CDS             complement(1039..2490)
                     /gene="panF"
                     /locus_tag="RHZ76_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HDV2812518.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(2480..2722)
                     /locus_tag="RHZ76_001867"
     CDS             complement(2480..2722)
                     /locus_tag="RHZ76_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HDV2812519.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLSGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(2831..4180)
                     /gene="accC"
                     /locus_tag="RHZ76_001868"
     CDS             complement(2831..4180)
                     /gene="accC"
                     /locus_tag="RHZ76_001868"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDV2812520.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(4191..4661)
                     /gene="accB"
                     /locus_tag="RHZ76_001869"
                     /gene_synonym="fabE"
     CDS             complement(4191..4661)
                     /gene="accB"
                     /locus_tag="RHZ76_001869"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDV2812521.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5527..6357)
                     /locus_tag="RHZ76_001870"
     CDS             complement(5527..6357)
                     /locus_tag="RHZ76_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812522.1"
                     /translation="MKSLFNRLTGKAVSRTAFVEHLGQEVVQHHPNWKVMISTDHKLM
                     RIDTGHQTVEITLDDAYKRYQAAPDPLQEVAADMLKQLAEMLPTGRCIVPVIKEKAWL
                     DTVRQQLSQYESDPEVLNASLDFAWEDFNDELIILFAEDGSETMRYLMRYEVTIDREK
                     AISNLKQILPEVTAEIVDTGANDGKVAVISAGGTYEPSLMLIDDLWVQSPDPECGDLL
                     VTVPSRGKLFFTHNDQPANLSALRQLTEQCFREDDHPLTTQIFIRRNGHFYPWVETLH
                     "
     gene            complement(6394..7368)
                     /locus_tag="RHZ76_001871"
     CDS             complement(6394..7368)
                     /locus_tag="RHZ76_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDV2812523.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRVLPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            7520..9460
                     /gene="csrD"
                     /locus_tag="RHZ76_001872"
     CDS             7520..9460
                     /gene="csrD"
                     /locus_tag="RHZ76_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HDV2812524.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSVLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            9765..10808
                     /gene="mreB"
                     /locus_tag="RHZ76_001873"
     CDS             9765..10808
                     /gene="mreB"
                     /locus_tag="RHZ76_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HDV2812525.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            10874..11977
                     /gene="mreC"
                     /locus_tag="RHZ76_001874"
     CDS             10874..11977
                     /gene="mreC"
                     /locus_tag="RHZ76_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDV2812526.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            11977..12465
                     /gene="mreD"
                     /locus_tag="RHZ76_001875"
     CDS             11977..12465
                     /gene="mreD"
                     /locus_tag="RHZ76_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDV2812527.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            12474..13067
                     /gene="yhdE"
                     /locus_tag="RHZ76_001876"
     CDS             12474..13067
                     /gene="yhdE"
                     /locus_tag="RHZ76_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HDV2812528.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKARAGVAQTAKDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASDEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            13057..14526
                     /gene="rng"
                     /locus_tag="RHZ76_001877"
     CDS             13057..14526
                     /gene="rng"
                     /locus_tag="RHZ76_001877"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HDV2812529.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            14594..18394
                     /gene="yhdP"
                     /locus_tag="RHZ76_001878"
     CDS             14594..18394
                     /gene="yhdP"
                     /locus_tag="RHZ76_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HDV2812530.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNNKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLASTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIVLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFDLTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCAECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            18824..20269
                     /gene="tldD"
                     /locus_tag="RHZ76_001879"
     CDS             18824..20269
                     /gene="tldD"
                     /locus_tag="RHZ76_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HDV2812531.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVCMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(20403..21332)
                     /gene="aaeR"
                     /locus_tag="RHZ76_001880"
     CDS             complement(20403..21332)
                     /gene="aaeR"
                     /locus_tag="RHZ76_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HDV2812532.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            21515..21718
                     /locus_tag="RHZ76_001881"
     CDS             21515..21718
                     /locus_tag="RHZ76_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HDV2812533.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            21726..22658
                     /gene="aaeA"
                     /locus_tag="RHZ76_001882"
     CDS             21726..22658
                     /gene="aaeA"
                     /locus_tag="RHZ76_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HDV2812534.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            22664..24631
                     /gene="aaeB"
                     /locus_tag="RHZ76_001883"
     CDS             22664..24631
                     /gene="aaeB"
                     /locus_tag="RHZ76_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HDV2812535.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            24723..24995
                     /locus_tag="RHZ76_001884"
     CDS             24723..24995
                     /locus_tag="RHZ76_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HDV2812536.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(25051..25314)
                     /gene="yhcN"
                     /locus_tag="RHZ76_001885"
     CDS             complement(25051..25314)
                     /gene="yhcN"
                     /locus_tag="RHZ76_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HDV2812537.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6011 a         6410 c         6864 g         6122 t
ORIGIN      
        1 ctcaaagaaa tttgagtaag ttctcaattt ttctttatga atgaaaattt tggcacgcaa
       61 atttgcgtaa ataatcggca aatttacgtg ccttttgtga gcttgctcgc acttcgcccc
      121 gcgtcaccct acggcgatgc gaaggttaat tcttacgacc ggtaatacgg caccactctt
      181 ctttttccac gaccgggtcc agtgcgaagc tatcggcata agcttcacaa acgctctctg
      241 cctggcttgc cagaataccg gaaaggccca gcaaaccgcc tgaaaccggc aggacgctga
      301 ttaacggtgc cagttcacgt aatgggcctg caaggatgtt agcgaccacc acgtcggctt
      361 tcatttcttc tggctgatct ttcggtaagt agagttccag acggtcagaa acgccattac
      421 gttcggcgtt atcgcggctg gcctgaatcg cctgcggatc gatatcaata ccaatggctt
      481 ttgctgcacc cagtttcagc gccgcgatcg ccagaatgcc ggaaccacag ccaaagtcga
      541 tgactgtttt accggttaaa tcgaggctgt cgagccattg caggcacaga gaggtggttg
      601 gatgggtacc cgtaccaaac gccagccctg gatctaacat cacgttgacg gcgttttcgt
      661 ccggcacatc acgccagcta gggcagatcc acagtcgttc accaaagcgc atcgggtgga
      721 aattatccat ccattcgcgc tcccagtctt tatcttctag ttgttcgatt ttatgcgcga
      781 agcctgcgcc gagcagcgga tggttttcca gaatcgccac cacgtcgttc atatcggttt
      841 cagcgtcgaa cagaccaatc acatcggtgt cgccccacag gcgcgtttcg cccggcagcg
      901 gttcaaatac tggcgtatcg tgggtatcct gaaaagtgat agaaacggca cccgcttcca
      961 tcagcgcatc gctaagatct tccgcgttcg cgccggtggt gttcagtttc agttggatcc
     1021 aaggcatggc aaaactcttt atttatcagt agtcaaaacg gtagcttgcg ggacggatgt
     1081 accgaaacgg tttccgacca ggaaagccag caaacttagt agtaacgagg gcacgatagg
     1141 gtggaagccc aggtactgaa tattcagcgt cgcgagtacg gcatacagca cgccgccaac
     1201 gatcatcgca cttagcgcgc ctttggcgtt ggcgcgttcc cagtaaagac ccagcaccag
     1261 cggccacagg aaaacggctt ccagcccacc gaaggccaac aaattcagcc agatgatcat
     1321 ttctggcggc ttccaggcgg caagcagcag caacgcgccg agaactaacg taattaccgc
     1381 cgacatccgc ttcagacgcg tctcgttttg catttgatcc ggacggatat tcagatagag
     1441 atctttaatg atcgtagcgg aactttgcag cagttgggcg ttaattgtcg acatgatcgc
     1501 agccatcggt gcagccagga agatcccggc agcaaacggt ggcagcactt ttaccattaa
     1561 cgttgggatc accaggtccg gtacggtgag atcggggatc accgcccgac ctaacgctcc
     1621 ggccaggtgc ataccgaaca tcagaattgc gaccacaatc gtaccgatga tgatcccccg
     1681 atgtacggct ttgctgtctt tataagagat acagcgcacc gcagtatgcg gcaggccaat
     1741 cacgccaaaa cacaccagta cccagaacga cgtcataaag gcaggcgaca gaatatcgtc
     1801 agcgccttgt ggcgtaacca gttgcggatc gatggtttgc aaggtctgta ctgcgttact
     1861 taagccgcca gcggcatgta ctacgccaat aagcagcaca acggtgccaa tcagcatcac
     1921 aagcccttgc atggtgtcgt tcagcacgct ggcgcgaaag ccaccaaagg cggtatataa
     1981 cgcaatgctg ataccaaaaa tcagcagccc ggtttcataa ggaatacccg ccgcggtttc
     2041 cagcaggcgc gcaccgccga taaactgcac ggtcatcgca ccaacgaacg caaccagcaa
     2101 actcaaactc gccagccaca ccagaagacg actctggtag cgggcaaaca gcatatcgtt
     2161 cagcgtcact gcattgtagc ggcgcgcaag aatcgcaaac ttcttgccga gaataccgag
     2221 tgaaagccag actgcaggaa gctgaatcat cgccagcaat acccagccca gcccgtattt
     2281 ataagcagct cctggcccgc cgataaacga actggcactg atataggtcg cggtgagcgt
     2341 catcgccagc acaataccgc ccatagagcg gctgccgagg aaatactcat taaggaaggt
     2401 gccggtgctc cgtttacgca tcgcataaac cgagataccg aacaccacca ccagataggc
     2461 gaccagcggt agaattactt caagctgcat cgtcatcctc cagtgggata tcgcgataga
     2521 taaatttcac catcgcccag cacagtccaa taaacagcag cggcgtcagg atgcaggcca
     2581 tctcaaacca gcgcggaaag ccggtaaaac cgggggcaac gccagataag taagcggcta
     2641 ctaaccaaac tgccagatac aaaagggtca gccccagcgc ccagcgcgcc tctttatggg
     2701 cctgaacaaa acgagtgtcc attttttgtc cctgatgggt gaagaaagcg gggattgtac
     2761 cttatggggg ttgtcgatcc ccagtaataa aaaaggccgg aaaatccggc cttttgacgc
     2821 tttagcagtc ttatttttcc tgaagaccga gttttttctc cagatagtgg atgttagtgc
     2881 caccatgctg gaagttctcg tcattcatga tgcggatctg cagatcaacg ttggttttga
     2941 taccgtcgat gatcagctcc tgcagcgcat tcttcatgcg ggcaatcgcc acgtcacggt
     3001 tttcaccgta gcaaatcagc ttaccgatca ttgagtcata gtacggcggt acggtgtagc
     3061 ccgcgtagat atgagactcc caacgtacgc caaaaccgcc aggtgcgtgg aaacgggtga
     3121 ttttgcccgg acttggcagg aaggtgttcg gatcttcggc gttgatacga cattccaccg
     3181 catggccgcg aacgtgaact tcttcttgct tgatcgacag cggttgaccg gcagcgatac
     3241 gcagctgttc tttgatcagg tcaacgccgg tgatcatttc tgtaaccggg tgttctacct
     3301 gaatacgggt gttcatttcg atgaaataga actcgccgtt ttcgaacagg aactcgaaag
     3361 tacctgcacc gcgatagccg atatcaacac acgctttagc gcaacgttcg ccgatgtagc
     3421 gacgcagttc cggggtaatg cccggtgctg gcgcttcttc gaccactttc tggtggcggc
     3481 gttgcatgga gcagtcacgt tccgccagat agatagcgtt gccctgaccg tcagccagta
     3541 cctgaatctc gacgtggcga ggattttcca ggtatttctc catgtaaacc atatcgttgc
     3601 tgaaagcagc tttcgcttcc gcacgggtca tggagatgga ttgtgccagt tcagcgtcgc
     3661 cgcgcactac gcgcataccg cgaccgccgc cgccgccgga ggctttgata atcaccggat
     3721 aaccaatgcg tttagcaatg gcacggtttt tatccatatc gtcgcccagc gggccgtcag
     3781 aacccggtac gcaagggacg cccgcttttt tcatcgccgc gattgcggat actttgtcgc
     3841 ccatcaggcg aatggtttct gctttcgggc caatgaagat aaagccggag cgttcaacct
     3901 gctcggcaaa gttggcgttc tcggagagga agccgtaacc cggatggatt gctactgcgc
     3961 cggtgatttc agcggcgctg atgattgccg ggatgttcag ataacttttt actgacggag
     4021 cagggccaat acagaccgtt tcatctgcca gtaatacgtg ttttagatcg cgatccgcgc
     4081 tggagtgcac agcgacagtc ttgatgccca gttctttaca ggcacgaaga atacgcaatg
     4141 caatctcgcc gcggttggca ataacaattt tatccagcat gttcgcctcg ttactcgatg
     4201 acgaccagcg gctcgtcaaa ttctaccggt tgtccacttt cgaccagaat tgctttcacg
     4261 gtaccggatt tgtccgcttc gatctggttc atcattttca tggcttcaac gatgcacagg
     4321 gtatcgccca cgttgacttt ctgacccact tcgatgaacg cttttgcgtc cgggcttggg
     4381 gtgcggtaga aagtaccaac catcggggaa cgtacgatgt gaccactgat ttccgctgct
     4441 gctggcgctt ccatggaagg aacggtcgcc ggagcggctg cgttagattg agctggctgc
     4501 tgcatcattg gtgcagcgta agcttgttgc atcacaggga aacttgcggc aggagctgca
     4561 cggctaatgc gtactgactc ttcgccttca gaaatttcca gttcggagat gcctgattct
     4621 tcaaccagct cgatcagttt tttaatctta cgaatatcca tgagtgggtt ccgtactctt
     4681 tgtttagtgt gattgtgaca ggcgtttcac cgccgtctgt aaagcgtatg ccccgacagc
     4741 tgtatgcata gcgataaatt ccagcaggcc gggacgccgg tctattttgc cccagagtgt
     4801 caatgttaga cttgacggac attgtgcagc gatctgctgc tatcctccgg caaaaaacaa
     4861 aatatacctt cgttgcgcca atgtcacctt ttccgcagcg caaaaactgc gtcagggagg
     4921 acgaggtcgc acattataac gatttcgtag caattggcag ctaaatactg gtcttatcag
     4981 ggaagataat caacagctaa catgtaaata accttcaaca ccgtgtaatt tgcaacaagc
     5041 cgcacaattc acgaaattag cgccatcatc gacggaactt cttataacgt aaggtgaaaa
     5101 gctgtaaagc cagccctgcg aagatgaggg gctgcaatga gataatcttc acagaccaca
     5161 aataatgtag ggatgccagg atcgcgacaa gatagacgac attacgcagc ggttgctggg
     5221 gattacccaa ttttcactgc atcgtctgga tagatgtgaa tgctaaagca cgcaaaatta
     5281 accgattgac aatgctcagc attaaatagg ttcgagtgat taatttttag tcaaacaaaa
     5341 tcagattatt ccccccattt tgcagaaggt aaactgtttt gtcaggcggc agtgcggcta
     5401 tctgatgctt tcagaatttg ccacctgaca ataaaaaagc cgatttggta gcaaaatcgg
     5461 cttcggtgag tttgcagttt tcatcacata ctccgacaag ctttttcaag catgacggag
     5521 gttgcgttaa tgcaaggttt ctacccaggg ataaaagtga ccgttacggc ggatgaatat
     5581 ctgtgttgtc agaggatggt catcctcacg gaagcattgt tctgttaact gtcgtaatgc
     5641 actgagattt gctggctgat cattgtgggt gaaaaacagt tttcctctgg agggaacggt
     5701 cactaacagg tcgccgcatt caggatcggg tgattgcacc caaagatcgt caatgagcat
     5761 cagactgggc tcataagtac cccctgcgga gataacggca acttttccat cgtttgcgcc
     5821 tgtatcaacg atttcagccg taacttccgg caatatttgc ttcaaattac tgatggcttt
     5881 ttcgcggtca atggtcactt cgtagcgcat cagatagcgc atggtttcgc taccatcttc
     5941 agcaaacaag ataattaact catcgttaaa gtcttcccag gcaaaatcca gcgaggcgtt
     6001 gaggacctct ggatcagact catactggct aagctgttgg cgaacggtat ccagccaggc
     6061 tttttcttta atcaccggaa caatacagcg cccggtcggc agcatttctg caagctgttt
     6121 cagcatatct gcagccactt cttgcagcgg gtctggtgcg gcttgataac gtttgtaggc
     6181 atcatcaagg gtgatttcca ccgtttggtg acccgtatca atgcgcatca atttatggtc
     6241 agtcgaaatc atgactttcc agtttggatg atgttgtaca acttcctgac caagatgttc
     6301 gacgaaagct gtccggctaa ccgcttttcc cgttaaccga ttgaataatg atttcaaaat
     6361 gagttccctc tcttattatt ccttgctaaa tggttagtta accttcacca gcgtgcgacc
     6421 ctggatctgg ttattaatga tggcctcggc aaacttcggt gcctctgcca gagatatctc
     6481 ttttgccgcc tgggtataga atgattccgg taaatcggcg accagtcgct gccaggcttg
     6541 tgcgcggcgt tctggtggcg tcattactga atccacccct tgcaaacgga cattacgcag
     6601 aataaatggc atgaccgtgg ttggcagagt aaaaccaccc gccagaccac aggccgccac
     6661 gcagccgccg taattcattt gcgccagcac ttttgccagc actttgtcgc caacggtgtc
     6721 aattgcccca gcccagactt gtttttccag aggacgggat tcggcaaact catcacgagg
     6781 gagaacacgg ctagcaccta aacttttcag atattcatgg gtactttcgc gaccggaaac
     6841 ggcaacgacc tgataaccca acttatgcag cagcgccacg gcggtactgc cgacgccacc
     6901 actggcaccc gtcacgacaa tctccccgtc ctgcgggcga acaccggcat cttccagcgc
     6961 catcacacac agcatggcgg taaaaccggc agtaccgata atcattgctt tacgcgcgtc
     7021 cagcccttgc ggcatggcaa ccagccagtc acctttcact cgcgcctgct ccgccagccc
     7081 accccagtgg ttttcaccaa cgccccagcc agtgagtaac acctcctgac cggcatgaaa
     7141 acgcggatct tcgctggtgc gtacagttcc ggcaaaatcg atcccaggaa tcatcggaaa
     7201 attacggatg atttttccct taccggtaat cgccagcgca tctttatagt tcaggctcga
     7261 ccagtgaaca tcgaccgtga catcgccctc cggcaggcga ctttcgtcca gagtctgtac
     7321 tgatgcgaga gttttgccgt cctgctgttc taaaagtaac gcctgcataa gtggtcctca
     7381 tgtgcatgat ggattggaaa ataatttctg aagactatac tcgctaatgg aaaagcaaag
     7441 ccgatgaagc gcaataaatt gcgagataaa tctgatttgc tagtatgccc gcttcctcac
     7501 tatcggagtt aacacaagga tgagattaac gacgaaattt tcggcctttg ttacgctgct
     7561 caccgggtta acaatttttg tgactttgct gggctgttcg ctaagtttct acaacgccat
     7621 tcagtataag tttagtcatc gcgttcaggc ggtggcgacg gcgattgata cccaccttgt
     7681 gtcgaatgac ttcagcgtat taaggccaca aattaccgaa ttaatgatgt cggcagatat
     7741 cgttcgtgta gacctgctcc atggtgataa acaggtttat accctggcca gaaatggtag
     7801 ttatcgtcca gttggctcca gcgatctgtt tcgcgaactg agcgttccgt tgataaagca
     7861 tccggggatg tcgttgcgtc tggtttatca ggatccgatg ggcaactatt tccattcgtt
     7921 gatgaccacc gcgccgctca cgggggcgat tggctttatc attgttatgc tcttcctggc
     7981 ggtacgctgg ttacaacggc aacttgccgg gcaagaattg ctggaaaccc gggctactcg
     8041 tatcttaaac ggtgagcgtg gctctaatgt gttgggaacc atctatgaat ggccgcccag
     8101 aaccagcagt gcgctggata cgctgcttcg tgaaattcag aacgcacgcg aacaacacag
     8161 ccgtcttgat acgctgatcc gctcttatgc cgcccaggac gtgaaaaccg gcctcaataa
     8221 ccgactcttt ttcgataatc agttagcaac gttactggaa gatcaggaga aagtaggtac
     8281 ccacgggatc gtgatgatga ttcgtctgcc ggatttcaat atgttgagcg atacctgggg
     8341 gcacagccag gttgaagaac agttcttcac tctgacgaat ctgctgtcga catttatgat
     8401 gcgctaccct ggcgcactgc tggcgcgtta ccaccgcagt gattttgctg cgctgttacc
     8461 gcaccggacg ttaaaagagg cagagagcat cgccggtcag ttaatcaaag ccgttgatac
     8521 cttgccgaac aataaaatgc tcgatcgcga cgatatgatc cacattggta tctgcgcctg
     8581 gcgtagtggt caggataccg agcaggtaat ggaacatgca gagtctgcca cgcgtaatgc
     8641 gggattgcag ggcggcaata gctgggctat ttacgatgac tcgttgcctg aaaaaggacg
     8701 cggtaatgtt cgctggcgta cgcttatcga gcaaatgctc agtcgcggcg gcccgcgcct
     8761 ttatcaaaaa ccggcggtta ctcgcgaagg tcaggttcat catcgcgaac tcatgtgccg
     8821 catcttcgat ggtaatgaag aggttagctc ggcggagtat atgccgatgg tcttgcagtt
     8881 tggcttatcg gaagagtatg accgtctgca aatcagccgt cttattccac tattgcgtta
     8941 ctggccagag gaaaatctgg cgattcaggt taccgttgag tcgctgattc gcccgcgttt
     9001 tcagcgttgg ctgcgcgata cgttaatgca atgtgaaaaa tcacaacgaa aacgcataat
     9061 tattgaactt gcagaggccg atgtaggtca acatatcagt cgtttacaac ctgttattcg
     9121 tttagtgaat gctttagggg tacgggtagc cgtcaaccag gctggtttga cgctggtaag
     9181 taccagttgg atcaaagaac ttaatgttga gttactcaag ctccatccgg ggctggtcag
     9241 aaacattgag aagcgaacgg agaaccagct gctggttcaa agcctggtgg aagcctgctc
     9301 cgggaccagc acccaggttt acgccaccgg cgtgcgttcg cgaagcgagt ggcagaccct
     9361 gattcagcgc ggtgttacag gcgggcaagg ggattttttc gcgtcctcac agccacttga
     9421 tactaacgtg aaaaaatatt cacaaagata ctcggtttaa cctgccgttt aatccgtttt
     9481 cacgtagaat aacgcgcgct gcgtctcatg ggagtgtgct tgtctgctcg ccagattgtt
     9541 gcagcacata tgcagatgaa tgaccttacg cggttgcaaa caggcgagga atgctgctga
     9601 tgcattaagc ctttctggac tcaggcagag atttgtaaca aaggaaacga actgcactaa
     9661 ttttcaccgt agcagatgat ttttgcgcct tgtcgctgct gcgtgtggtt ggtaaagtaa
     9721 gcggattttc ttttccgccc cagctttcag gattatccct tagtatgttg aaaaaatttc
     9781 gtggcatgtt ttccaatgac ttgtccattg acctgggtac tgcgaatacc ctcatttatg
     9841 taaaaggaca aggcatcgta ttgaatgagc cttccgtggt ggccattcgt caggatcgtg
     9901 ccggttcacc gaaaagcgta gctgcagtag gtcatgacgc gaagcagatg ctgggccgta
     9961 cgccgggcaa tattgctgcc attcgcccaa tgaaagacgg cgttatcgcc gacttcttcg
    10021 tgactgaaaa aatgctccag cacttcatca aacaagtgca cagcaacagc tttatgcgtc
    10081 caagcccgcg cgttctggtt tgtgtgccgg ttggcgcgac ccaggttgaa cgccgcgcaa
    10141 ttcgtgaatc cgcgcagggc gctggtgccc gtgaagtctt cctgattgaa gaaccgatgg
    10201 ctgccgcaat tggtgctggc ctgccggttt ctgaagcgac cggttctatg gtggttgata
    10261 tcggtggtgg taccactgaa gttgctgtta tctccttgaa cggtgtggtt tactcctctt
    10321 ctgtgcgcat tggtggtgac cgtttcgacg aagctatcat caactatgtg cgtcgtaatt
    10381 acggttctct gatcggtgaa gccaccgcag aacgtatcaa gcacgaaatc ggttcggctt
    10441 atccgggcga tgaagtccgt gaaatcgaag ttcgtggccg taacctggca gaaggtgttc
    10501 cacgcggttt taccctgaac tccaatgaaa tcctcgaagc actgcaggaa ccgctgaccg
    10561 gtattgtgag cgcggtaatg gttgcactgg aacagtgccc gccggaactg gcttccgaca
    10621 tctccgagcg cggcatggtg ctcaccggtg gtggcgcact gctgcgtaac cttgaccgtt
    10681 tgttaatgga agaaaccggc attccagtcg ttgttgctga agacccgctg acctgtgtgg
    10741 cgcgcggtgg cggcaaagcg ctggaaatga tcgacatgca cggcggcgac ctgttcagcg
    10801 aagagtaatc ggatgcaggc aggggaagtg tctgtttacc ctgcctggtc tgatacgaga
    10861 atacgcataa cttatgaagc caatttttag ccgtggcccg tcgctacaga ttcgccttat
    10921 tctggcggtg ctggtggcgc tcggcattat tattgccgac agccgcctgg ggacgttcag
    10981 tcaaatccgt acttatatgg ataccgccgt cagtcctttc tactttgttt ccaatgctcc
    11041 tcgtgaattg ctggatggcg tatcgcagac gctggcctcg cgtgaccaat tagaacttga
    11101 aaaccgggcg ttacgtcagg aactgttgct gaaaaacagt gaactgctga tgcttggaca
    11161 atacaaacag gagaacgcgc gtctgcgcga gctgctgggt tccccgctgc gtcaggatga
    11221 gcagaaaatg gtgactcagg ttatctccac ggttaacgat ccttatagcg atcaagttgt
    11281 tatcgataaa ggtagcgtta atggcgttta tgaaggccag ccggtcatca gcgacaaagg
    11341 tgttgttggt caggtggtgg ccgtcgctaa actgaccagt cgcgtgctgc tgatttgtga
    11401 tgcgacccac gcgctgccaa tccaggtgct gcgcaacgat atccgcgtaa ttgcagccgg
    11461 taacggttgt acggatgatt tgcagcttga gcatctgccg gcgaatacgg atattcgtgt
    11521 tggtgatgtg ctggtgactt ccggtctggg cggtcgtttc ccggaaggct atccggtcgc
    11581 ggttgtctct tccgtaaaac tcgataccca gcgcgcttat actgtgattc aggcgcgtcc
    11641 gactgcaggg ctgcaacgtt tgcgttatct gctgctgctg tggggggcag atcgtaacgg
    11701 cgctaacccg atgacgccgg aagaggtgca tcgtgttgct aatgaacgtc tgatgcagat
    11761 gatgccgcag gtattgcctt cgccagacgc gatggggcca aagttacctg aaccggcaac
    11821 ggggatcgct cagccgactc cgcagcaacc ggcgacagga aatgcagcta ctgcgcctgc
    11881 tgcgccgaca cagcctgctg ctaatcgctc tccacaaagg gctacgccgc cgcaaagtgg
    11941 tgctcaaccg cctgcgcgtg cgccgggagg gcaatagtgg cgagctatcg tagccaggga
    12001 cgctgggtaa tctggctctc tttcctcatt gcgctgttgc tgcaaatcat gccctggccg
    12061 gataacctga ttgttttccg gccaaactgg gtgttactca tcttgttgta ttggatcctg
    12121 gccttgcctc atcgcgtaaa tgtgggcaca ggttttgtga tgggtgccat actggatctg
    12181 atcagcggct cgacgcttgg cgtacgcgta ttggcgatga gcatcattgc ttacctggtg
    12241 gcgctgaaat accagctttt ccgcaacctc gcattatggc agcaggcgct ggtcgtcatg
    12301 ttgctttcgc tggtggtgga tattattgtt ttctgggcag agtttttagt gattaacgtc
    12361 tctttcagac cggaagtgtt ctggagtagt gtagtcaatg gggtgctctg gccgtggatt
    12421 ttcttgctga tgcgcaaagt ccgtcagcag tttgcagtgc aataaaggtt tctatgactt
    12481 ctctgtattt agcttccggt tctccgcgtc gtcaggagtt acttgcgcaa cttggcgtga
    12541 cctttgaacg tattgttacg ggcattgagg agcagcgtca gccgcaggag agcgcgcagc
    12601 agtatgttgt gcgtctggcg cgcgagaaag cacgggcagg tgtcgcgcaa acggcgaagg
    12661 atctcccggt gctgggtgcg gatactatcg ttatcctgaa cggagaagtg ctggagaaac
    12721 cgcgcgacgc agagcatgcg gcgcagatgt tgcgcaaatt atcgggtcag acccatcagg
    12781 tgatgacagc agtggcgttg gccgacagcc agcacattct cgattgcctg gtggtcaccg
    12841 atgtgacttt cagaacgtta acagacgaag acatcgcggg ctatgtcgcc agcgatgaac
    12901 cgttagataa agcaggtgca tacggtattc aggggctggg tggctgtttt gtcaggaaga
    12961 taaatggcag ctatcacgcc gtagtcggct taccgctggt tgaaacgtat gaattattaa
    13021 gtaattttaa cgcactgcgt gagaaaaggg ataaacatga cggctgaatt gttagtaaac
    13081 gtaacgcctt cggaaacgcg agtggcgtat attgatggcg gtattctgca ggaaattcat
    13141 attgaacgtg aggcgcgacg cggaatagta ggcaatatct acaagggtcg tgtaagtcgt
    13201 gtacttccgg gtatgcaggc ggcttttgta gatattgggc tggataaagc cgcgtttctt
    13261 catgcatccg acatcatgcc gcacaccgaa tgtgtggcgg gtgaagaaca aaagcaattc
    13321 acggtgcgcg acatctcgga actggttcgt caggggcaag atctgatggt gcaggtggtg
    13381 aaagatccgc ttggcactaa aggtgcgcgc ctgaccaccg atatcacgct cccttctcgc
    13441 tatctggtgt ttatgccagg ggcttctcac gttggggttt cccaacgtat tgaaagcgaa
    13501 tcagaacgtg aacgcctgaa aaaagtggtc gcagagtatt gcgacgagca gggcgggttt
    13561 atcatccgta ccgcagcgga aggggttggc gaggctgaac tggcctccga tgccgcttat
    13621 ctgaaacgcg tctggaccaa agttatggag cgtaaaaaac gcccgcagac ccgttatcag
    13681 ctgtacggcg aactggcgct ggcgcagcgt gttctgcgtg atttcgccga tgccgaactg
    13741 gaccgcattc gcgttgactc acgcctgact tacgaagcgt tacttgagtt cacctcggag
    13801 tacattcccg agatgacaag caagctggag cattacacag gacgccagcc gattttcgat
    13861 ctctttgatg tcgaaaacga aatccagcga gcgctggaac gcaaagtaga actgaaatcc
    13921 ggtggttatc tcattatcga ccagaccgaa gcgatgacca ccgtggacat caataccgga
    13981 gcgtttgtcg gtcatcgcaa tctggacgac accattttca ataccaatat tgaagcgacg
    14041 caggctatcg ctcgccagtt acggttgcgt aatctgggcg ggattatcat tattgatttc
    14101 atcgatatga ataatgaaga tcaccgccgc cgagtgctgc actcgctgga gcaggcgttg
    14161 agcaaagacc gggtgaaaac cagcgttaat ggtttttcgg cgctggggct ggtggagatg
    14221 acgcgtaaac gcacccgcga aagcattgag cacgtactgt gtaacgaatg cccaacctgc
    14281 cacggtcgcg gaacggtgaa aaccgtggaa acggtatgct atgaaatcat gcgcgagatt
    14341 gttcgtgtcc accatgctta cgactccgac cgtttcctgg tctatgcttc tccggcagta
    14401 gctgaagcct tgaaaggcga agagtcacac tcgctggcgg aagtggaaat tttcgttggc
    14461 aaacaggtta aagtacaaat tgaaccgctc tataaccagg agcagtttga cgtcgtaatg
    14521 atgtaaacag atgctgggcc gccatccggc aaagggtttt tgagtcacat ttttagcaga
    14581 caaggagtga cgggtgaggc gattgccggg gattttactg cttactggag ccgcgctcgt
    14641 tgtgatcgct gccctgctgg ttagcggcct gcgtattgct ttaccgcatc ttgacgcctg
    14701 gcgtccggaa atcctcaaca aaatagaatc cgcgactggc atgccggtag aagccagtca
    14761 gctctcagcc agctggcaga attttggccc gacgcttgaa gcacacgaca tccgtgcaga
    14821 actaaaagat ggcggcgaat tttcggttaa acgcgttact ctggcgctgg atgtctggca
    14881 gagcctgtta catatgcgct ggcagtttcg cgacctcact ttctggcagc tgcgctttcg
    14941 caccaacact cctatcacca gcggtggtag tgatgacagt ctggaagcca gtcacatcag
    15001 cgatctgttt cttcgtcaat ttgaccattt cgatcttcgc gacagtgaag tcagtttcct
    15061 gacgccatcc ggtcagcgcg ccgagctggc gatcccacaa ctcacctggc tgaacgatcc
    15121 acgtcgacac cgtgcggaag gcctggtaag cctctccagc cttaccggac agcacggcgt
    15181 gatgcaggtg cgcatggatt tgcgcgatga tgaggggttg ttaagcaatg gtcgcgtctg
    15241 gctccaggcg gatgacatcg acctgaagcc gtggctcggt aaatggatgc aggacaatat
    15301 tgcgctggaa acggcacagt tctcccttga aggctggatg acgatcgaca aaggcgatgt
    15361 aaccggcggt gacgtctggc tgaaacaggg cggtgccagc tggttgggcg agaagcaaac
    15421 gcatacgctg tcggtggata atctgaccgc gcatattacg cgtgaaaatc cgggctggca
    15481 gttctctatt cccgatacac ggatcacgat ggacggcaaa ccctggccga gcggagcatt
    15541 gacgctggcc tggataccgg aacaggacgt tggcggcaaa gacaataaac gcagtgacga
    15601 actccggatt cgcgccagta atctggagct ggcaggcctg gagggcatac gcccgctggc
    15661 cgcgaaactt tcacctgcac tgggtgatgt ttggcgctcc acacaaccga gcggcaagat
    15721 taacactctg gcgctggata tcccgcttca ggcggcagac aagacccgtt ttcaggcatc
    15781 gtggagcgat ctggcctgga agcaatggaa attattaccg ggtgcggaac acttctccgg
    15841 gacgctttcc ggcagcgttg aaaatggttt gcttaccgcg tcgatgaagc aggcaaagat
    15901 gccttacgaa acggtattcc gtgcgccact agaaatcgcc gacggccagg caactataag
    15961 ctggctgaac aataacaaag gtttccagct ggatgggcgt aatattgacg ttaaagccaa
    16021 agccgtccat gcgcgcggcg gttttcgtta cctgcaacct gctaacgatg aaccctggct
    16081 gggtattctg gctggcatca gtaccgatga tggttcacaa gcctggcgct atttcccgga
    16141 aaacttgatg ggtaaagacc tggttgatta cttaagtggc gcgattcagg gcggtgaagc
    16201 ggataacgcg acgctggttt atggtggcaa tccgcaactc ttcccctata aacacaacga
    16261 aggtcagttt gaagtgctgg tgccgctgcg caacgcgaag tttgccttcc agccggactg
    16321 gcctgcatta actaaccttg atattgaact ggactttatt aacgacggtt tatggatgaa
    16381 aaccgatggc gttaatctgg gcggcgtgcg cgcgagtaat cttaccgcag tgatccctga
    16441 ctactcaaaa gaaaaactgc tgattgacgc tgacattaaa ggtccgggta aagccgttgg
    16501 cccttacttt gatgagacac cgctgaaaga ttctctgggt gcgaccctgc aagaactcca
    16561 gctcgacggc gatgtgaatg ctcgcttaca tcttgatatc ccgctgaacg gcgagctggt
    16621 aaccgcgaaa ggtgaagtga cgctgcgtaa taacagtctg tttatcaaac cactcgccag
    16681 caccctgaaa aatttgagcg gtaaattcag ctttatcaat ggcgatctgc aaagtgaacc
    16741 actgacagca agctggttta atcagccgtt gaacgtggat ttttccacca aagaaggggc
    16801 aaaagcctac caggtagcgg taaacctcaa cggtaactgg caaccggcga aaaccggcgt
    16861 tctgcctgca gcggtgaacg aagcattgag tggcagcgtg gcgtgggatg gtaaagtggg
    16921 cattgttctg ccttatcatg ctggcgcgac gtataacgta gagctaaacg gcgatttgaa
    16981 gaatgtgagc agtcacttac cttcaccgtt agccaaacct gcgggtgaac cactgccggt
    17041 aaacgttaag gttgatggca atctcaacag ctttgattta accggacagg ctggtgcgga
    17101 taaccatttc aatagccgct ggttgctcgg tcaaaagctg acgctcgacc gtgctatttg
    17161 ggcggcagac agtaaaacgc tcccgccgtt gccggaacaa agtggtgttg aactcaatat
    17221 gccgccgatg aatggtgccg agtggctggc cctgtttcag aaaggtgcgg cggagagtgt
    17281 cggtggtgca gcgagtttcc cacaacacat aacgttacgt acgcctatgt tgtcgctggg
    17341 aaatcagcaa tggaataacc tgagtattgt ttcgcaaccg acggcaaatg gcacccaggt
    17401 tgaggcgcaa gggcgtgaaa tcaacgccac gctggcgatg cgtaataacg cgccgtggct
    17461 ggcgaatatc aaatatcttt attacaaccc gagcgtggcg aaaactcgtg gtgattcaac
    17521 gccgtcatca cctttcccga caacggagcg cattaacttc cgtggctggc cggacgcaca
    17581 aatacgatgc gcagagtgct ggttctgggg gcaaaaattc ggtcgtattg acagtgatct
    17641 caccatttct ggcgatacat taacgctgac caatggactg attgatactg gtttctcgcg
    17701 gcttactgcc gatggtgaat gggttaataa tccggggaat gaacgtacct cgctgaaagg
    17761 aaaactgcgc gggcagaaaa ttgatgccgc cgcagaattt tttggtgtca cgacgcccat
    17821 acggcagtcg tcatttaatg tggattacga tttacactgg cgtaaagcac cgtggcagcc
    17881 agatgaggcg acgttgaatg gcatcattca tactcaactg ggtaaaggcg aaattaccga
    17941 aatcaatacc ggacatgccg ggcaattgct gcgcttattg agcgtagatg ccctgatgcg
    18001 taagctgcgt tttgatttca gagacacttt tggcgaaggg ttctattttg actccattcg
    18061 cagcaccgcg tggattaaag acggcgttat gcacaccgac gacacgctgg tggatggcct
    18121 ggaggcggat atcgccatga aagggtcggt aaatctggta cgtcgcgacc tgaatatgga
    18181 agcggttgtc gcaccagaga tttctgcgac ggtgggcgtg gctgcggctt ttgcggttaa
    18241 ccccattgtt ggcgcggcag tgtttgccgc cagtaaagtg ctggggccgc tgtggagcaa
    18301 agtctccatt ttgcgctatc acatttcggg tccgctggac gatccgcaaa tcaacgaagt
    18361 gttgcgccaa ccgcgtaaag aaaaagcgca atgatttgac gagggcgcgt aattgcccca
    18421 atctcatagg ataatcgttg ccaaaggcca acgagccaga acataaccgt aggtcggata
    18481 gggcgttcac gccgcatccg gcagccgttg cctgatgcga cgctgtgcgt cttatcaggc
    18541 ctacaaacgg aacataaccg taggtcggat aaggcgttta cgccgcatcc ggcagccgtt
    18601 gcctgatgcg acgctgtgcg tcttatcagg cctacaaacg gaacataacc gtaggtcgga
    18661 taaggcgttt acgccgcatc cgacagccgt tgcctgatgc gacgctgtgc gtcttatcag
    18721 gcctacaaac cggaacataa ccgtaggctg gatagggcgt tcacgccgca tccggcagcc
    18781 gtaaaaaatc ctctactgca gtaactaacg agtagcaaaa acgatgagtc ttaacctggt
    18841 aagtgaacaa ttgctagcgg cgaacggcct gaaacatcag gacttgttcg cgatcctcgg
    18901 tcaactggcc gaacgtcgcc ttgattatgg cgatctctat tttcagtcga gctatcacga
    18961 atcctgggtt ttagaagacc gcattattaa agatggttct tacaacatcg atcagggcgt
    19021 tggtgtgcgt gcaatcagcg gtgaaaaaac cggatttgct tacgctgacc aaatcagcct
    19081 gctggcgctg gaacagagtg cgcaagcggc gcgcaccatc gtccgtgata gtggtgatgg
    19141 taaagtacag acgctgggcg cggtagagca tagcccgttg tatacctcgg tagatccgct
    19201 gcaaagcatg agccgtgaag agaagctgga tatcctgcgt cgcgtcgata aggttgcccg
    19261 cgaagcggac aagcgcgtac aggaagtgac tgccagcctc agtggtgtct atgaattaat
    19321 tttggttgcg gccaccgacg gcacgctagc ggcggatgtc cgtccgctgg tgcgtctttc
    19381 cgtgagcgtt ctcgtcgaag aagatggcaa acgcgaacgc ggtgccagtg gcggcggcgg
    19441 tcgttttggt tatgaattct tccttgccga tctcgacggc gaagtccgtg cggatgcatg
    19501 ggcaaaagaa gcagtgtgta tggcgctggt caatctttct gccgttgctg caccagcggg
    19561 caccatgccg gtagtacttg gcgcaggttg gccgggcgtg ctgttgcatg aagcggttgg
    19621 tcacggtctg gaaggcgact tcaaccgccg tggcacttca gtatttagtg gacaggtcgg
    19681 ggagctggtg gcttcagaac tgtgtaccgt ggttgatgat ggcacgatgg tcgatcgccg
    19741 aggttcggtg gcgattgatg acgaaggtac gccaggccag tacaacgtgc tgattgagaa
    19801 cggcattctg aaaggctaca tgcaggataa actcaacgcg cgtttgatgg ggatgacgcc
    19861 gactggcaac ggtcgccgtg aatcctacgc ccatctgccc atgccgcgta tgaccaacac
    19921 ctatatgctg ccgggtaaat cgaccccgca ggaaattatt gaatccgttg agtacggtat
    19981 ctatgcaccg aactttggtg gcggtcaggt ggatatcacc tccggcaaat tcgttttctc
    20041 cacttcagaa gcatatctga ttgaaaacgg taaagtaacg aagccggtga aaggcgcaac
    20101 gttgattggt tccggtatcg aaaccatgca gcagatttcg atggttggca acgacctgaa
    20161 actggataac ggcgtgggtg tctgcggtaa agaagggcaa agtttgccgg ttggcgtggg
    20221 ccagccaacg ttgaaagtcg ataacctgac tgttggcggt actgcgtaat aatttcacta
    20281 ttttcagaag gataattaat ctttctacgt gcacgaacgg tcccctcgcc cctttgggga
    20341 gagggttagg gtgaggggaa cccgttggca caggtttgaa caacgtaaca gtacaatatg
    20401 aattacttct ctttcccgcg cccatgcatc tcctgaaaca atttaccgac ctcaacaaaa
    20461 taatccgtca gcgagttgat cacgacctgt accttcagcg gcagtttatc tttttcggta
    20521 tacaacgcat aaaccgggcg tggatctgac tggtaacgcg gcagcaggat ctccagctcc
    20581 ccacgattga tctcgttgat cacccacatc agcggcacgt aggcgatccc ggcacccgcc
    20641 gtcagccagc gcaccagcgt catcggatca ttagtcacaa atcttccttg tgggatcagg
    20701 cgagtcgaga tcccttccgg tgcgatcagt tcaaattcat tgtcgggccg cacgctgtat
    20761 tcaagccatg aatgactact caaatcggcg ggtttttccg gtatgccgta ttgtgtgaga
    20821 tagcttttcg cggcgcacac caccatcggc atcgcgccca gacggcggga aaacaggctg
    20881 gaatcctgca acgcgccgac gcggattacc acatccagac catcggcaat caggtcgggg
    20941 gcaggaattc cggtaaccag attgacgctc aaacctgggt attctttcag catttttgct
    21001 gtcagcccgg cgagaacatt ttgtgccata gttgaagaac agccaatgcg cagcgtcccg
    21061 atgggggtgt tattgaaggc atacagttgc tcatgaacat cctgcacttc atggagcatc
    21121 cgacggcagc cctggtagta aattctgccg gcttcagtca gaccaatgct gcgtgtacta
    21181 cggtttaaca gctttacctg caactcatct tccagttttg acaccgtctg actgatggac
    21241 gaaacgctca tctgtagctg tctggcggcg gcggtaaaag agccaaattc aactactttg
    21301 gcaaacaccg acatgcgttt tagtcgttcc attattcact ctgacttaaa agtgatttag
    21361 atcacataat atagataaca gcataacagt tacgctaata tattaaatat caatctacag
    21421 caatgttgct ctcgcccggc tttccatgcc cttctctgcg gcgacagatg ctgaaaataa
    21481 taacgcctgc tctctctata caaccaaggt cgacatgagt ctgtttcccg ttatcgtggt
    21541 gtttgggctg tccttcccac cgatattttt tgaattgctt ttatcactgg cgattttctg
    21601 gctggtgcgc cgggtacttg tgccaacagg tatctacgac tttgtctggc atccggcgtt
    21661 gttcaacacc gcgctctatt gctgcttgtt ttatttgata tcgcgactgt tcgtttgagg
    21721 ttgaagtgaa aacactaata agaaaattct cccgtacggc catcacggtc gtattagtca
    21781 ttctggcctt catcgcaatt tttaatgcct gggtctatta caccgaatcc ccgtggacgc
    21841 gtgacgcgcg ctttagcgct gatgtcgttg cgatcgcgcc ggacgtttct ggactcatta
    21901 cccaggtgaa tgttcatgat aaccagctgg tgaaaaaagg acaggtactg ttcaccatcg
    21961 accagccgcg ctatcaaaag gcgcttgagg aagcgcaagc cgatgttgct tattatcagg
    22021 tactggcaca ggagaaacgc caggaggccg gacgtcgtaa tcgtctcggt gtgcaggcga
    22081 tgtctcgcga agagatcgac caggccaaca acgtactaca aacggttctg catcagttag
    22141 cgaaagcgca ggcgacccgc gatctggcaa aactggatct tgaacgcacg gtgatccgcg
    22201 cgccagcgga tggctgggtg accaacctca acgtctatac cggtgagttt attactcgtg
    22261 gatcaacggc ggttgcgctg gtgaaacaga actctttcta tgtactggcc tatatggaag
    22321 aaactaagct ggaaggggtg cgccctgggt atcgtgcaga gatcacgccg cttggcagta
    22381 acaaagtgct gaaagggact gttgatagtg ttgccgctgg ggtcaccaac gccagcagca
    22441 cgcgtgacga caaagggatg gcgaccatcg actctaacct tgaatgggtg cgtttggcgc
    22501 aacgtgttcc ggttcgtatt cgtctcgaca accagcaaga gaacatctgg cctgcgggca
    22561 ccactgctac agtggtggtc actggcaaac aagatcgcga cgaaagccag gattcgttct
    22621 tccgtaaaat ggcccatcgc ctgcgtgagt ttggttaatc acgatgggta ttttctccat
    22681 tgctaaccaa catattcgct ttgcggtaaa actggcgacc gccattgtac tggcgctgtt
    22741 tgttggcttt cacttccagc tggaaacgcc acgctgggcg gtactgacag cggcgattgt
    22801 tgctgccggt ccggcctttg ctgcgggagg tgaaccgtat tctggcgcta ttcgctatcg
    22861 tggctttttg cgcatcatcg gcacatttat tggctgtatt gccggactgg tgatcatcat
    22921 tgcgatgatc cgcgcaccat tattgatgat tctggtgtgc tgtatctggg ccggtttttg
    22981 tacctggata tcctcgctgg tacgaataga aaactcgtat gcgtgggggc tggccggtta
    23041 taccgcgctg atcattgtga tcaccattca gcctgaacca ttgcttacgc cgcagtttgc
    23101 cgtcgaacgt tgtagcgaga tcgttatcgg tattgtgtgt gcaattatgg cggatttgct
    23161 cttttctccg cgatcgatca aacaagaagt ggatcgagag ctggaaagtt tgctggtcgc
    23221 gcaatatcaa ttaatgcaac tctgtatcaa gcatggcgat ggtgaagttg tcgataaagc
    23281 ctggggcgac ctggttcgac gcaccacggc gctacaaggt atgcgcagca acctgaatat
    23341 ggaatcttcc cgctgggcgc gggccaatcg acgtttaaaa gcgatcaata cgctatcgct
    23401 gacgctgatt acccaatcct gcgaaactta tcttattcag aatacgcgcc cggaattgat
    23461 cactgatact ttccgcgaat tttttgacac accggtagaa accgcgcagg acgtccacaa
    23521 gcagctcaaa cgcctgcgaa gagttatcgc ctggaccggg gaacgggaaa cgcctgtcac
    23581 catttatagc tgggtcgcgg cggcaacgcg ttatcagctt ctcaagcgcg gcgttatcag
    23641 taacacaaaa atcaacgcca ccgaagaaga gatcctgcaa ggcgaaccgg aagtaaaagt
    23701 agagtcagcc gaacgtcatc atgcaatggt taacttctgg cgaaccacac tttcctgcat
    23761 tctgggaacg cttttctggc tgtggacggg ctggacttcc ggtagtggtg caatggtgat
    23821 gattgcggtc gtgacgtcac tggcaatgcg tttgccgaat ccacgcatgg tggcgatcga
    23881 ctttatctac gggacgctgg ccgcgctgcc gttagggctg ctctactttt tggtgattat
    23941 ccctaatacc caacagagca tgttgctgct gtgtattagc ctggcagtgc tgggattctt
    24001 cctcggtata gaagtacaga aacggcgact gggctcgatg ggggcactgg ccagcaccat
    24061 aaatattatc gtgctggata acccgatgac tttccatttc agtcagtttc tcgacagcgc
    24121 attagggcaa atcgtcggct gtgtgctcgc gttcaccgtt attttgctgg tgcgggataa
    24181 atcgcgcgac aggactggac gtgtactgct taatcagttt gtttctgccg ctgtttccgc
    24241 gatgactacc aatgtggcac gtcgtaaaga gaaccacctc ccggcacttt atcagcagct
    24301 gtttttgctg atgaataagt tcccagggga tttgccgaaa tttcgcctgg cgctgacgat
    24361 gattatcgcc caccagcgcc tgcgtgatgc gccgatcccg gttaacgagg atttatcggc
    24421 gtttcaccga caaatgcgcc gcacagcaga ccatgtgata tctgcccgta gcgatgataa
    24481 acgtcgtcgg tactttggtc agttgctgga agaactggaa atctaccagg aaaagctacg
    24541 catctggcaa gctccaccgc aggtgacgga accggttcat cggctggcgg ggatgctcca
    24601 taagtatcaa catgcgttga ccgatagtta agtcaaaacc gacgccaaaa gcgtcggttt
    24661 tttcatggct atacttagcg atgcacggca gaactcgccg cgaaacgtga cggtggcaac
    24721 agatgaatat ttataccttt gattttgatg agattgagag tcaggaggat ttttatcgtg
    24781 actttagcca aacctttggt ctggcgaaag ataaggtacg cgatctcgac tcactatggg
    24841 atgtgttaat gaacgatgtc ctgccgctac cacttgagat tgaatttgtt catctgggag
    24901 agaaaacgcg tcgccgtttt ggcgcgttaa tattgctgtt tgatgaggca gaggaagagc
    24961 tggaagggca tttgcgtttt aatgttcgtc attagcgcaa aaaaaagccc ccgaaccggg
    25021 ggcaatatcg tcggacaaga cgatgagggt ttatttgtac agttcagccg tagcgtgcca
    25081 ggtgtcaccg ctacgagctt cagtaatctg gtaggccgtt gcgcctttct cttccgcttt
    25141 tttgttcagc atttcacgca tatccattgg cgaagacgcc acaccactta cggatacggt
    25201 cccgattgct tcacgatttt gtgcttgtgc agcatcaatg gagtcggcag cgaatgcacc
    25261 gaaagagaga acagaaagta cgcttaatgc agcaacagtg gttttgattt tcatgatttt
    25321 tacctcgaca taatctttta gctgggcctt tgtttcgtga cccatatcac aaaatcaagt
    25381 atacactaat tattgagcta attaata
//
LOCUS       DAPHBJ010000052        25359 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.052, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000052 DAPHBJ010000000
VERSION     DAPHBJ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.052"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            197..616
                     /locus_tag="RHZ76_001886"
     CDS             197..616
                     /locus_tag="RHZ76_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HDV2812538.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            616..1884
                     /gene="umuC"
                     /locus_tag="RHZ76_001887"
     CDS             616..1884
                     /gene="umuC"
                     /locus_tag="RHZ76_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HDV2812539.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(1930..2460)
                     /gene="dsbB"
                     /locus_tag="RHZ76_001888"
     CDS             complement(1930..2460)
                     /gene="dsbB"
                     /locus_tag="RHZ76_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HDV2812540.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2606..4147)
                     /gene="nhaB"
                     /locus_tag="RHZ76_001889"
     CDS             complement(2606..4147)
                     /gene="nhaB"
                     /locus_tag="RHZ76_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HDV2812541.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKASGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4369..5088
                     /gene="fadR"
                     /locus_tag="RHZ76_001890"
     CDS             4369..5088
                     /gene="fadR"
                     /locus_tag="RHZ76_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HDV2812542.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5140..6672)
                     /locus_tag="RHZ76_001891"
     CDS             complement(5140..6672)
                     /locus_tag="RHZ76_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HDV2812543.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            7002..8300
                     /gene="dadA"
                     /locus_tag="RHZ76_001892"
     CDS             7002..8300
                     /gene="dadA"
                     /locus_tag="RHZ76_001892"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HDV2812544.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8310..9380
                     /gene="dadX"
                     /locus_tag="RHZ76_001893"
     CDS             8310..9380
                     /gene="dadX"
                     /locus_tag="RHZ76_001893"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HDV2812545.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAATHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(9766..11502)
                     /locus_tag="RHZ76_001894"
     CDS             complement(9766..11502)
                     /locus_tag="RHZ76_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDV2812546.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQHHESNISWMFIVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFSLTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11597..12511)
                     /gene="ldcA"
                     /locus_tag="RHZ76_001895"
     CDS             complement(11597..12511)
                     /gene="ldcA"
                     /locus_tag="RHZ76_001895"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HDV2812547.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTSNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            12611..13222
                     /gene="emtA"
                     /locus_tag="RHZ76_001896"
     CDS             12611..13222
                     /gene="emtA"
                     /locus_tag="RHZ76_001896"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HDV2812548.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13224..13958)
                     /locus_tag="RHZ76_001897"
     CDS             complement(13224..13958)
                     /locus_tag="RHZ76_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HDV2812549.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKLL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14159..14413
                     /locus_tag="RHZ76_001898"
     CDS             14159..14413
                     /locus_tag="RHZ76_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDV2812550.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            14591..15031
                     /locus_tag="RHZ76_001899"
     CDS             14591..15031
                     /locus_tag="RHZ76_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812551.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(15110..16807)
                     /gene="treA"
                     /locus_tag="RHZ76_001900"
     CDS             complement(15110..16807)
                     /gene="treA"
                     /locus_tag="RHZ76_001900"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDV2812552.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(17127..18545)
                     /gene="dhaM"
                     /locus_tag="RHZ76_001901"
     CDS             complement(17127..18545)
                     /gene="dhaM"
                     /locus_tag="RHZ76_001901"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HDV2812553.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(18556..19188)
                     /gene="dhaL"
                     /locus_tag="RHZ76_001902"
     CDS             complement(18556..19188)
                     /gene="dhaL"
                     /locus_tag="RHZ76_001902"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HDV2812554.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(19199..20269)
                     /gene="dhaK"
                     /locus_tag="RHZ76_001903"
     CDS             complement(19199..20269)
                     /gene="dhaK"
                     /locus_tag="RHZ76_001903"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDV2812555.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLVNGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            20497..22416
                     /gene="dhaR"
                     /locus_tag="RHZ76_001904"
     CDS             20497..22416
                     /gene="dhaR"
                     /locus_tag="RHZ76_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HDV2812556.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(22516..25272)
                     /locus_tag="RHZ76_001905"
     CDS             complement(22516..25272)
                     /locus_tag="RHZ76_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV2812557.1"
                     /translation="MIFSPLLIAADTGSQYGTNITINDGDRITGDTADPSGNLYSVMT
                     PAGNTPGNINLGNDVTVNVNDASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINL
                     IGDYTHADLGTGSTIKSNDDGIIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGS
                     GSKITTDGSTGVYIGGLNGNNANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTN
                     STIKTNGDNAHGLWSFGQVSANALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGG
                     LVTSGSDAIINFTGTAAQRNSIFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGL
                     WALSGGRITGDSLAITGAAGARGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGY
                     AASRINASGRMLINGSVLSKGGLINLDMHPGSVWTSSSLSDNVNGGKLDVAMNNSVWN
                     VTSNSNLDTLALSHSTVDFASHGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNN
                     KGDLLNISGSSAGNHVLAIRNQGSEATTGNEVLTVVKTTDGTASFSASSQVELGGYLY
                     DVRKNGTNWELYASGTVPEPTPNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGG
                     NYLNVGYLLNYVENRTLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYS
                     GIQFGGDKRLSDVMPLYVDLYIGSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSD
                     LVIKASRQKNSFHVLDSQNNGVNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQL
                     IYSHQNEMTMKASNGLNIHLNHYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFS
                     GDTEYLLNNSREKYSFKGNGWNNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGY
                     RFSF"
BASE COUNT         6257 a         6321 c         6563 g         6218 t
ORIGIN      
        1 ctattttttt gccgggtgat atttttcatt tctgctggat gagcgtcgtc gccagaaggc
       61 cacgtgagca caggataaga gaacgaaaaa tcagcagtct atgcagcgac aaatattgat
      121 agcctgaatc agtattgatc tgctggcaag aacagactac tgtatataaa aacagtataa
      181 cttcaggcag attattatgt tgtttatcaa gcctgcggat ctccgcgaaa ttgtgacttt
      241 tccgctattt agcgatcttg ttcagtgtgg ctttccttca ccggcagcag attacgttga
      301 acagcgcatc gatctgaatc aactgttgat ccagcatccc agcgcgactt acttcgtcaa
      361 agcaagtggt gattctatga ttgatggtgg aattagtgac ggtgatttac tgattgtcga
      421 tagcgctatt accgccagcc atggtgatat tgtcatcgct gctgttgacg gcgagtttac
      481 ggtgaaaaaa ttgcaactac gcccgacggt acagcttatt cccatgaaca gtgcgtactc
      541 gcccattacc atcagtagcg aagatacgct ggatgtcttt ggtgtggtga tccacgtcgt
      601 taaggcgatg cgctgatgtt tgctctctgt gatgtaaacg cgttttatgc cagctgtgag
      661 acggtgtttc gccctgattt atggggtaaa ccggtggttg tgctatcgaa taatgacggt
      721 tgcgttatcg cccgaaacgc tgaggcaaag gcgctgggtg ttaaaatggg cgatccctgg
      781 ttcaaacaaa aagatctgtt tcgtcgctgt ggtgtggttt gctttagcag caattatgag
      841 ctttacgcag acatgagcaa tcgggtgatg tcgacgctgg aagagctatc gccccgcgtc
      901 gagatttaca gtattgatga ggcattctgc gatctgacag gtgtgcgtaa ttgtcgcgat
      961 ctgactgatt ttggcagaga aattcgcgca acggtgctac aacgtaccca tcttactgtt
     1021 ggcgtgggga tcgcccagac caaaacgctg gctaagcttg ccaatcatgc ggcaaaaaaa
     1081 tggcagcggc agacgggtgg ggtggtggat ttatcaaatc tggaacgcca gcgtaaatta
     1141 atgtctgctc tccccgtgga tgacgtctgg gggattggac ggcggatcag caaaaaactg
     1201 gacgcgatgg ggatcaaaac cgttctcgat ttggcggata cagatatccg gtttatccgt
     1261 aaacatttta atgtcgtgct cgaaagaacg gtgcgtgaac tgcgcggcga accctgtttg
     1321 caactggaag agtttgcacc gacgaagcag gaaattatct gttcccgctc gtttggtgaa
     1381 cgcatcacgg attatccgtc gatgcggcag gccatttgta gttacgctgc ccgggcggcg
     1441 gaaaaacttc gcagcgagca tcaatattgt cggtttatct ccacgtttat taagacgtca
     1501 ccatttgcgc tcaatgaacc ttattacggc aatagcgcgt cggtaaaact gctgacgccc
     1561 actcaggaca gcagggatat cattaacgct gctacgcgat ctctggatgc catctggcaa
     1621 gcgggccatc gttaccaaaa agcgggcgtg atgctggggg atttcttcag tcagggagtc
     1681 gcgcagctca atttattcga tgacaacgca ccgcgccccg ggagtgagca attgatgacg
     1741 gtaatggata cactgaatgc taaagagggc agaggaacac tctattttgc cgggcagggg
     1801 atccagcaac aatggcagat gaagcgagcc atgctttcac cacgttatac aacgcgaagt
     1861 tctgatttac tgcgggtcaa ataaatatag cggcaggaaa aaagcgctcc cgcaggagcg
     1921 ccgaatggat tagcgaccga acagatcacg ttttttcgct ttaaacggct gggaaatcac
     1981 caccagcact gcgacaatca ggtaagcgat aaaaataccg agcagccact gcggcatttc
     2041 cagacctaaa aaatcccact gacgctcggc gcaatcgcca gaggcgacaa acacttgcgg
     2101 cacccactta tccagcggca gccattccgg gaaacgaacc ataaaatcac aggtggcaaa
     2161 cggcgaagga tagagctgaa gcatggtgtg ctcgtaagtt aactgcacac cgcggaacgc
     2221 actatacaac cagataacca tcgctacata acgcagtggc gtttttgggg cgatcgcgcc
     2281 aatcagcgca gcacccagaa cgccgaataa cgcgcagcgt tcataaatac agagcacgca
     2341 aggtttcagt aacatcacat gctggaacca cagcgccgtc agttccagtg ccagagcagt
     2401 aaacgccatc aacagccacg cgccccggcc ttgtgaacat tggttcaaaa atcgcaacat
     2461 aatcatttcc ctgcaatatg catagagtgc gcagtttaaa ccaattcatt cgctgcgcca
     2521 ccaggaggca gcaaaaatag gacgcaattg gacaattatc gggcgaaaag gcaaaccggg
     2581 catttatatg cccggtaagt tgttatcaaa gcgttgctat ccagcccatt tgcataaacc
     2641 attcggttac aggggcaagc gtaaactcga cgcagagcag tccgacgagt gtcaggacga
     2701 gggtgtaagg cagggccatc cacaccatgc ggccataaga gaggcgaatc aatggcgcga
     2761 gtgcagaggt cagcaggaac aggaacgcag cctgaccgtt cggcgtagcg acagagggca
     2821 gattggtacc ggtattaatg gcgaccgcca gcagctcgta ttgcttcaac gtgatagcgc
     2881 cactttccat tgccgctttc gcttcgttga tataaatcgt ccccacgaag acgttatccg
     2941 aaatggatga cagcagaccg ttgaaaatat agaacagcga cagctgagca tgttccgatg
     3001 cctgcaacac aaactgaata atcggcgaaa acagttgttg gtcgataatc accgcgacta
     3061 ccgagaaaaa caccgtcaac agtgcggtga atggcagaga ttcggtgaag gctttaccga
     3121 tagcatgttc atcggtgaca ccggtcaatg atgtcgccag aataatgact gacaaaccaa
     3181 tcaagccaac ttccgccaga tgcaacgcca gcgcagtcac cagccagacg ccaataatcg
     3241 cctggacaat cagacggatt ttatcctgac gggtacgctg gtggcggctt tgatcgtcaa
     3301 actgttgcaa cacttcgcgg actttctccg gcagcgtttc accgtagcca aaccaacgca
     3361 gcttctctac cagcaggcag gttaacaggc cacaaatcag aaccggaacg gtcaccggcg
     3421 acatgcgcag gaagaaatcg ccaaaatgcc agccagacgc tttagcgatg atcaggttct
     3481 gtggttcgcc caccatggtc atcacgccgc ctaatgcggt gccgacaccg gcatgcatca
     3541 tcaggctacg cagaaagcca cggaactgtt ccagaaccac tttgtaatgc ttgtcgatat
     3601 gactatcgtc ttgcaggtcg gtatcttcag tacgggaaga ggctacgcgg tgataaatac
     3661 cataaaaacc gactgcaacg ctgatcacca ccgccacgac ggttaaggca tcgaggaacg
     3721 cggagaggaa cgccgccgcc acgcaaaaag agagcgacag cagcattttg gagcgaatgc
     3781 ttaacagcaa acgggtaaat atgaacagca atagctgttt cataaaatag atacccgcca
     3841 ccataaacat cagtaacagc aagacttcaa gatttgccgc cacctcttca cggacgtgtt
     3901 ccgcgctggt catgccgatg aataccgctt cgatagccaa cagaccaccg gggagcagcg
     3961 ggtagcattt cagggccatc gccagagtga aaataaattc cgctaccagc aaccagcccg
     4021 cgacgaaagg gctgatgagg aaaattaacg ggtttacgat taagaaaatg atgagggcga
     4081 gtttgtacca gtcgggggac tggcccaaaa aattgcgcca tagcgcgcgg ccccaggaga
     4141 tctccatgat ggtttccctt accttacaaa taatcaatga tgtttttatg tttaaacgca
     4201 aagcttaacg gtcaggcagg agtgaggcaa gtcttgatag tcaaggggaa agagatgcgg
     4261 aaaatgaagc cttgatccct tttttcttct ttttgtctgc tatcagcgta gttagccctc
     4321 tggtatgatg agtccaactt tgttttgctg tgttatggaa atctcactat ggtcattaag
     4381 gcgcaaagcc cggcgggttt cgcggaagag tacattattg aaagtatctg gaataaccgc
     4441 ttccctcccg ggactatttt gcccgcagaa cgtgaacttt cagaattaat tggcgtaacg
     4501 cgtactacgt tacgtgaagt gttacagcgt ctggcacgag atggctggtt gaccattcaa
     4561 catggcaagc cgacgaaggt gaataatttc tgggaaacct ctggtttaaa tatccttgaa
     4621 acactggcgc gactggatca cgaaagtgtg ccgcagctta ttgataattt gctgtcggtg
     4681 cgtaccaata tttcaactat ttttattcgc accgcgtttc gtcagcatcc cgataaagcg
     4741 caggaagtgc tggctaccgc taatgaagtg gccgatcacg ccgacgcctt tgccgagctg
     4801 gattacaaca tattccgcgg cctggcgttt gcttccggca acccgattta cggtctgatt
     4861 cttaacggga tgaaagggct gtatacgcgt attggtcgtc actatttcgc caatccggaa
     4921 gcgcgcagtc tggcgctggg cttctaccac aaactgtcgg cgttgtgcag tgaaggcgcg
     4981 cacgatcagg tgtacgaaac agtgcgtcgc tatgggcatg agagtggtga gatttggcac
     5041 cggatgcaga aaaatctgcc gggtgattta gccattcagg ggcgataatc ccttccgttt
     5101 aaagagcaaa cccctcaaac gaggggtttt ttgttgtttt tacagatttc ccattcttgg
     5161 cgggcaacgt tccagcaact cgatgctgcc gtcttcgttt tgctgttcga gcatcacatc
     5221 aaatccccac aggcgatgca catgcttcag cacttctttg cgcccccgat ccagcggtgc
     5281 gcgattatgt ggaatgtaac gcagcgtcag cgaacggtcg ccgcgcaaat ccacgttcca
     5341 gatctgaata ttcggctcca gattacttaa gttatattgc gacgataacc ggttacggat
     5401 ctcccgataa ccttcttcat tatgaatagc ggaaatctcc agataattat gccgatcatc
     5461 atccagcacg gtgaagaagc ggaaatcacg catcactttc ggtgacagaa actggctgat
     5521 aaaactctca tctttgaaat cacgcatcgc gaaatgcagc gtttccagcc agtcggaacc
     5581 ggcgatatcc gggaaccagt atttgtcttc ttccgttggc gactgacaaa tccgtttaat
     5641 atcctggaac atggcgaacc cgagggcata cgggttgatg ccgctgtacc acgggctgtt
     5701 ataggggggc tggaagacca cattggtgtg gctgtgcaaa aactccagca taaaacgttc
     5761 cgttactttc ccttcatcat acagatggtt aaggatggta tagtgccaga aggtcgccca
     5821 gccttcgttc atcacctgag tctgtttttg cggataaaaa tactggctca ccttacgcac
     5881 aatacgcagg atttcacgct gccatgattc cagcagtggc gcatttttct ccataaaata
     5941 gagcaggttt tcttgtggtt cggacggata gcggcgcgct tcagcaaccg ttttctcttc
     6001 ctcgcgcttc ggcagggtac gccagagcat attgacctga ctttgtagat actcttcgcg
     6061 acttttctgc cgggctttct cttcttgcag cgagattttt tgcgggcgtt tgtaccggtc
     6121 cacgccgtag ttcatcagcg cgtggcacga gtccagaagc cgttctactt catcaacgcc
     6181 ataacgttct tcgcactcgg taatatattt acgggcaaaa atcagataat cgacaatcga
     6241 actggcgtcg gtccaactgc ggaataagta attgttttta aagaaagagt tatgcccata
     6301 gcaggcgtgt gccatcacca gcgcctgcat ggtaatggtg ttctcttcca tcaggtaagc
     6361 gatacacggg ttagagttaa tgacgatttc ataggccagt ccttgctgac cgtgcttata
     6421 cagccgttca gtctcgataa actttttacc gaatgaccag tgcggatagt taattggcat
     6481 gccgacgctg gagtaggcat ccatcatctg ttctgaggtt atcacttcaa tctggtgcgg
     6541 gtaggtatcc agccggtaga gtttcgccac ccggtctatc tctgccagat aaacatccag
     6601 caggtcgaac gtccagtcgg gtccatcgct caaacgtgtg gtgtccttat tcatagaatc
     6661 gatcgtcgcc atacgcgcac ctcattgttg tcggcgctct ctgtgtggag cacctcattt
     6721 caagcataga acacctgtta aaaaccgcgt cgccggagaa tttttttctt tgcgatttct
     6781 tattatcaga gtgccactaa tccgcttctg aacggaattt tatgctggat aaaaagggcg
     6841 ttcagcagga gatactaaag acgccatatt gccgcagagt cagggagatg tgagccagct
     6901 caccataaaa aagccgtatg ttgaataata ttttcaactg agttatcaag atgtaattag
     6961 attattattc ttttactgta tctaccgtta tcggagtggc tatgcgagtt gtcatactgg
     7021 gaagtggtgt ggtaggcgtt gccagcgcct ggtacttaaa tcaggcagga catgaggtca
     7081 ccgtcattga tcgggagccg ggggccgccc tggaaaccag tgccgcgaat gccgggcaaa
     7141 tctcccccgg atatgctgca ccgtgggcgg caccaggtgt gcctttaaaa gcgattaaat
     7201 ggatgttcca gcgccatgcg ccgctggcgg ttcgtctcga cggtacgcag ttccagttga
     7261 aatggatgtg gcaaatgtta cgtaactgcg acaccagcca ctacatggaa aacaaagggc
     7321 ggatggtgcg tctggcggaa tacagccgtg attgcctgaa agcattgcgc gccgaaacca
     7381 atattcagta tgaagggcgt cagggtggga cgctgcaact gttccgtacc gaacaacagt
     7441 atgaaaatgc gacccgcgat atcgccgtgc tggaagatgc cggcgtaccg tatcagctgc
     7501 tggaatccag ccgcctggcg gaagtggagc ccgcgctggc agaagtggcg cacaaactga
     7561 cgggcggcct gcagttaccc aatgatgaaa ccggagactg tcagctattt acccagaatc
     7621 tggcgcggat ggcggagcag gcgggggtta aattccgctt taatacgccc gttgaccaac
     7681 tgctttgcga cggcgagcaa atctacggcg tgaagtgtgg cgatgaagtg attaaggccg
     7741 atgcgtatgt gatggcgttt ggttcttact cgacggcgat gctcaaaggc attgttgata
     7801 ttccggtgta tccgctgaaa ggctactcgc tgaccattcc aattgcgcag gaagatggtg
     7861 cgccggtatc caccattctt gatgaaacct acaaaatcgc cattacccgt ttcgataacc
     7921 gcattcgtgt tggcggaatg gcggagattg ttggttttaa taccgagctg ttgcaaccgc
     7981 gtcgtgaaac gctggagatg gtggttcgcg atctctatcc acgcggcggt catgtcgagc
     8041 aggcgacttt ctggactggt ctgcgcccga tgacgccaga cggcacgccg gttgtcgggc
     8101 gtacacgctt taaaaatctg tggctgaata ccggtcacgg cacgctcggc tggacgatgg
     8161 cttgcggttc cggtcagttg ttaagcgatc tgctctctgg tcgcacgcca gcgatcccat
     8221 atgaggatct aagcgtagcg cgctacagcc gtggatttac gccatcacgt ccgggccatt
     8281 tacatggcgc acacagctaa ggaaacgaga tgacccgtcc gatacaggcc agcctcgatc
     8341 tgcaggcatt aaaacagaat ctgtccattg tccgccaggc cgcgacgcac gcgcgcgtct
     8401 ggtcggtggt aaaagcgaac gcttacgggc atggtattga gcgtatctgg agcgcgctcg
     8461 gggccaccga tggctttgca ttgcttaacc tggaagaggc aataacgtta cgtgagcgcg
     8521 gctggaaagg accgatcctg atgctggaag gatttttcca tgctcaggat ctggagattt
     8581 atgaccagca ccgcctgacc acctgcgtac acagcaactg gcagctcaaa gcactgcaaa
     8641 atgcgcggct aaaagcaccg ttggatattt atcttaaagt gaacagtggg atgaatcggt
     8701 tgggcttcca gcccgatcgc gtgcttaccg tctggcagca gttgcgggca atggcgaatg
     8761 ttggcgaaat gaccctgatg tcgcattttg ccgaagcgga acatcctgat ggaatttccg
     8821 gcgcgatggc gcgtattgag caggcggcgg aggggctgga gtgtcggcgt tcgttgtcca
     8881 attcggcggc gactctgtgg cacccggaag cgcattttga ctgggttcgg cctggcatta
     8941 ttttgtatgg cgcttcgccg tccggtcagt ggcgtgatat cgccaatacc ggattacgtc
     9001 cggtgatgac gctaagcagt gagattattg gtgtccagac gctaaaagcg ggcgagcgtg
     9061 tgggctacgg cggtcgctat actgcgcgcg atgaacagcg aatcggcatt gtcgccgcag
     9121 ggtacgccga cggttatccg cgccacgcgc ctaccggtac ccctgtttta gtggacggcg
     9181 tgcgcaccat gacggtggga accgtctcga tggatatgct agcggtcgat ttaacgcctt
     9241 gcccgcaggc gggtattggt acgccggttg agctgtgggg caaggagatc aaaattgatg
     9301 atgtcgccgc cgctgccgga acggtgggct atgagttgat gtgcgcgctg gcgctacgcg
     9361 tcccggttgt gacggtgtaa cttgttgtaa gccggatcgg aggcaacgtc ttctgggtgc
     9421 aaaaaaatca tccatccggc tggtcagcaa ctgtagttgt taatgtgaca gagccattgc
     9481 ccatgatagt gtccattaaa aggatggaca ctatttcccc ggaacctgaa ctcaccgcac
     9541 aggcgttcta cataaaacgc ttacgcttca ttgttgactc gaactcgact tcagataaat
     9601 cacgctttac ccttgatgga gcctgtacat agatttgtgt aattgcctga ttttgatatg
     9661 ttcaattcaa catcaaatga aggttaaatt atggacgaca aacaattgca ggctcaggct
     9721 gcgttcagca aagcatcgca accggcgata gatgcttcat taaatttaag attcagcttc
     9781 ctcttcagcc acccgtacgc caatcttcaa cacttcatta tcttctttct cggccaccgt
     9841 ccagatcatc ccggcaaact ctacctggtc accgacaacc ggtgccgcgc ctaacaactg
     9901 ctggacaatt tcacccagcg tttgctgctt atcacgatat tctcgcccgt cttccaggcc
     9961 atatatcagc gcaacatcag catatttggc gctggcttcg agaatgaagt caccaaagaa
    10021 gcgttgatcc agcgcgaccg gcggcgactg gctgaacagt ttaccgagcg ccggaagatc
    10081 gcgttcccga ccaattacac acaacacatc gccttcgcgc agtcgggtgc taccggtagg
    10141 atgaagcaac tggttatcac gaaacagtgc cgcaatacgc gtctcttttg gcatatgcaa
    10201 atcgcgcagt gccgcgccca cgcaccattt atcggcactc aattgataaa caaactgctc
    10261 ccacggattt tccggatgaa tatccaggcc aacgcgtgac accggacgtc ccactggcgg
    10321 aaccaccact ttggcttttt tagccgccca cgagagtgat gttccctgca agagcagtga
    10381 aaccagaacc acaaagaagg cgacattaaa gaacagacgt gcattctcca gccccgccat
    10441 catcgggaac acggccagga tgattggcac cgcgccgcgt aatcctaccc agctgataaa
    10501 cacgcgctca cgcagattga aaccgcggaa ggggagcaat ccagcaaata ccgaaagggg
    10561 acgggcgaag aatatcatcc atgcggacaa aatgagcgcc ggaatggcaa taggcagcag
    10621 atcgcttggg ttaaccaaca gccccagcac caggaacatg gcgatttgcg ccagccaggc
    10681 gaggccgtcg aaattttgca ggatgccgta gcggttgcga atagggcgat tacccagcag
    10741 aaaaccgcac agatacaccg ccagaatacc gctaccttcc agcgcagtag ttaatgagaa
    10801 aatcagaata ccgccactta atgccagcaa tggatataat ccggcgggca gggcgatgcg
    10861 gttgatcatt tgcagtagta aataaccgcc gccaagacct attacaatgc ccagaccaaa
    10921 ttgttgcaga atatcgacaa tgaacatcca gctaatattg ctctcatgat gctggatcat
    10981 cgcaatcagg gtaatcgtca gaaagaccgc cattggatca ttactgccgg attcaatttc
    11041 cagcgtcgag ccaacacgtt cgttaagccc cttaccaccc agcagagaaa agaccgctgc
    11101 agcatcggta gagccgacga tagcgccgat taataagcct tcaatcaaat caagattaaa
    11161 cagccacgcc gccatcatgc cggttaaacc agaggtgata agcacgccca gcgtcgccag
    11221 cgacagtgcc ggtcctaacg ccacacgaaa ggaactggcc tgagtgcgca tcccgccgtc
    11281 gagcaaaata atcgccagtg ccaggttact taccatgtag gcgaaggggt aattatcaaa
    11341 cgggataccg ccgacgccat cgactcctgc cagcatgccg atcgccagaa aaataaccag
    11401 aataggaatg ccaagacggg aagaaaatga actaagtaat atactgctgg tgacaaggat
    11461 ggatcctaag atgaaaaggc taattattgt tgtggcatcc aacggtcggt tactcctgat
    11521 tacgctgtct cttatataaa ccctaccata ttagcggcag agacagcgtt ttacttagcc
    11581 ctgaagcgtg atttttttac attttaagaa caggatgacc gctaatcgtc aactgagtgc
    11641 cttcccgggt gttattcaga atggcatgtg cgcccaacgg cagcgtaacc gtgcgttgtt
    11701 catgaccaaa atcgaggccg gtaatgagtg gaatcgacag gcgggaacgc aaaaacgcgt
    11761 acactgactc caggttgtaa cctgcgtcat aatcattgga cgtgctgccg ctaaagctac
    11821 cgagaataat cgccttctga cgtggcaaaa ttcccgcatg atagagctgc aacagcatac
    11881 gttcgacccg gaaagggtgc tcgttaatat cttccagcac cagaataccg ttctcaattt
    11941 ttggcatcca cggtgtaccg atcagtgaaa tcagcatcgc aagattgcct ccccacaacg
    12001 tgccttcggc ccgacatgtc gggccttccc cttgccattc aatggtgaag gtttcattgc
    12061 gtaacgccag ccagaaatgg tgctcggtaa aggcgttcag ttcatccgcg ccaaagtttg
    12121 ctaccagcat ggggccgcta aaggtgatga cattgccatg cgccagaaga ccgcactgaa
    12181 tggcggtaaa atcgctatgt ccgcaaatga gcaacgggtc atgttgttgg cgagccacca
    12241 gtgcctgcca gtcaatatct gccagtaaac gactggcacc gtaaccgccg cgtacagcca
    12301 gcacgatggt gttgggggta gtcagtctgg caagggaatt gagatcttcc agacgctctg
    12361 tttccgtacc cgcaaaacgc tcacaacgac gggcaataac ctcgacgtta ttgacttgat
    12421 gccccgcgtc ggtaaggcgc tggataccac gcagcgcggc gtgctgttta atgcagtaac
    12481 ccgatggggc aattaagtga aacagagaca tggcaattcc ttgctgacaa cagaaacgaa
    12541 atgtatatca tgccgcttag gtgtgccgtt gtcacctcaa cggcgattcc aggctataag
    12601 gatagaagaa gtgaaattga gatggtttgc ctttttgatt gtgttattag cgggttgttc
    12661 atcaaagcat gactatacga acccgccgtg gaacgcgaaa gttccggtgc aacgtgcgat
    12721 gcagtggatg ccaataagcc agaaagccgg tgcagcctgg ggcgtcgatc cacaattgat
    12781 cacggcgatt atcgctatcg aatcgggtgg taatccgaac gcggtgagta aatcgaatgc
    12841 cattggtttg atgcagttaa aagcttcaac ctccggacgt gatgtttatc gccgtatggg
    12901 ctggagtggt gagccgacga ccagcgagct gaaaaatcca gagcgtaata tttcaatggg
    12961 ggcggcttac ctgaatattc tggaaaccgg cccgctggca ggcattgaag atccgaaggt
    13021 actgcaatat gcgctggtgg tgtcatacgc taacggggca ggtgcgctgc tacggacttt
    13081 ctcgtcagat cggaaaaagg cgatcagcaa aatcaacgat ttggatgctg acgagttcct
    13141 cgaacacgta gcgcgaaatc accctgcgcc gcaggctccg cgctatatct acaaacttga
    13201 gcaggcactg gacgcgatgt aaatcagtcg cgcactttgt ccgctttttc ccgggcttct
    13261 cgctcgagag agaaaataat ccgctgtaat tgccgctcca ccgtcgggct gacgttaaga
    13321 aaacggaagc tcagacgggg agtggtgatg gtttcattct tgccatcaat cactttgcgc
    13381 tcgctgatgg agattaactg ggcgtcaaag tgaaaaacac cccattgccc catgttgact
    13441 tcaatctgag cgaagcgcat gccttcttgt aattcggcag gctttgctgt ttccagtaat
    13501 gcgcccatgc cgcctaacga caaatcatac aggcggaaac gtaacgtact gttatccgcc
    13561 agtttggtct ggcaaaaata aggcggatgg agtggggcgg agatgcggaa atatcggcgt
    13621 cgttgtacaa accataaggt gggaggcggt acggtaataa atgccggaag ctgcaagtat
    13681 tcactctgct gtagttgttc aacagtaaac tcgactttcg caccctgagt ttcggcggta
    13741 atggtaatgt gctgtgcctt tagcacggcg atgttgtctt cggcttgact gccgaaatcc
    13801 agcaccagtt tatccggggt tattgccagt aatttgctga tcagctgccc gccattccaa
    13861 ctgagacgca aaggaattgc ggctttgtgc aaatcgcgta acacgcccag gacggctaac
    13921 ggattttgtt tcaggaactg ctcatggtaa tgactcacgc ggaaaaactc ctgactgaca
    13981 aactgtcttt ggtttatcgg tcacagttaa caaaacttaa tacaaatgcg tgaatatttt
    14041 ttacatgttg ttcttaaatc agccgacata cgcccagcat cgatcccgcc cttgcctata
    14101 cttagagcgt tgatgtaagc atttcttgcg tccgattcat cgaaacgagg gcctgaacat
    14161 gggaattatt gcctggatta tttttggcct gatagccggc attatcgcca agctaatcat
    14221 gccggggcgt gatggtggtg gatttttcct gacctgtatt ctcgggatag tcggtgcggt
    14281 ggtcggcggc tggctggcga ccatgtttgg cattggcggc tccatcagtg gttttaatct
    14341 gcacagcttc ctggtggcgg tggtgggagc tattctcgtt ctgggcatat tccgcctcct
    14401 gcgaagagaa taagattttc ataaggcgga tagcgataca gatgccgcta tccgctttca
    14461 catcagaacg tatactcgac acctgcttta cgggtgaaaa aaatcaatat agcacttcga
    14521 tatccgataa agtcggttcc cggtaacaac aacagaatcc ttttttaatt attgtttcgt
    14581 tgtttttggt gtgattcaga aaataaatat tttagacagt agccagtcag gcgttaaaaa
    14641 tggggcggat agtgaggcgg ttttggctgc gcttattgaa catttagaat tgatcaatcc
    14701 ttctggcaga ttaagccaaa atacccgttc ggcaatgctt cagttacgcg aagaggaatg
    14761 gtctgaattc tttttttggt tgctaaattc tctggagtgt ctggattatg tgattattaa
    14821 tttaactcca gaaagcaaaa aaacgttgat gagtgaacac cgtaacaata ttcaggtagc
    14881 aattgatgct ttatatagcc aacgtcggcg taaatcgccg ggtgatgaaa gtgaaacact
    14941 tacccgtaga aatgacgcta tcttcggcaa ccacgtatgg caaacatttg cgcaatactt
    15001 cccgccagga cttgaaaaac cgtctgtgta gaaaaaacca tagctatggt gttatctgcc
    15061 gacaacgcca tccggagaag ccgaatgacg ttgtcggagt aagcgctggt taaggtgtgg
    15121 gttgtgcctc tttggttgag ggttgcgtcg ttgctgactt aacggtcgga cgcgtcgccg
    15181 gaacattgtc acacggttgc tctttcgggc agatcaaatc cagcattttc agcgtcacgc
    15241 cattggtcca gccaaagcca tcctgtaatg gatattcgcc accgccgccc cccgttccgg
    15301 tggtgctgac atcatatttt tccaccagct ttttctcccg gtcataggtg tgctgaacat
    15361 tggtcaggaa gtgccagcta atgtccatcg ccacctcttt ttgcccgtag ttttgtaatc
    15421 cttctgtcgc gacccactgt aacggtgccc agccatttgg cgcatcccat tgttgcccac
    15481 ttttcaccga cgtggtgttc aggccgccgg gttgcagcag atgtgttttc gtcgccgtcg
    15541 ccattttgtt ggcgcgatct ttcgctgccg cattgacgta cagcgggaac agggcggccg
    15601 cagttaactg attgcgcact ttatgacttt tcaggtcgta atcggcatac cagccttgtt
    15661 gatcgttcca caggtatttt tcgatccctt tttgacgggc atttgccagc gtttcgtact
    15721 ggtttgccat cgcgttatct ccggcagctt tgctggcgcg ggcgaggatt ttttccattt
    15781 taaacatcag gctgttcaga tcgaccggta cgatgctggt ggtgcgtaag gtatttaact
    15841 gctgcgggtt gtccatccag cgcgagctga aatcccagcc agacgcagcg gcagagcgca
    15901 ggtcgcggta aatttcagtg gcaggtcgat tcggattgct tttggcggtg gcaatatctt
    15961 ccacccatga ctctggtcgt ggcgtatcgc gatcgtccca gtagcggttg agaagggtac
    16021 catcctgaag tttgacaacg cgtttttcct gttgtccggc ttgcaggttt tcaacaccgt
    16081 ccatccagta agcatattct ttttgcattt gcggcaggta ttgcttcaac gcggcatcgc
    16141 cttcatgctg cgccagtaac tctaccatca gggcaaagaa gggcggttgc gagcggctta
    16201 aatagtaact gcggttgccg ttgggaatat gaccgtaagt gtctatttca tgagcaaaat
    16261 tggccaccat atccgcgact ttatcccagt gaccgctttc ggcaagtcct aacatggtga
    16321 agtaactgtc ccagtaatat acctcgcgaa agcgtccgcc cggcacgaca taaggttccg
    16381 gcagcggtaa cagagaatcc catttttcgg tgttttcggt agaacgcgtt aataccggcc
    16441 aaagtccgtc aatatgttcg cgcagtgact gcccctctgg cggaacatat ttctcgcctt
    16501 ctttcggcag ggtgaaattg acgttaacga aatggcgcag atcaaatccg ctctggtttt
    16561 gctgcatccg ataatcagca aggatcatca gcggatcgct gttcggcacg gcatcggcaa
    16621 aggttttttg gtccggaaaa agtttggcgt tttgcacatc attaaacagc ggccctaata
    16681 aaatatcagg cggctgtggt gttaccggtg tttcttctgc ctgcaccgat agcgcagcga
    16741 aacacaaaaa gatacaggct ggaattaacg ccattttttg cgggcgagaa ggtgcggggg
    16801 atttcatcaa tcattctcct ttggcgaaac cgaataaaac ggtattcaag tatcagaaaa
    16861 ccttagttca ggatcgcact agctgcatga tcgaaatctc attctgcgaa ctgtcaggca
    16921 ttttagaaaa gggaattaaa gggataaata acggcgaaat agagtgatca aataacgtct
    16981 gtttattagc cagccagcgg cgttgggttc gtgattagcg aagaaaaatc atcataaatt
    17041 ggtgaaaaat ataacaggta gttaaaaacc attagtgctg agtaaattgc cggatgacat
    17101 cagaacgatg ccatccgaac agtggcttaa ccctgacggt tgaaacgttg cgttttaacg
    17161 tccagcgtta gcgtttcttc tggttgtatc gcatacagtt tctcaccctg ctggcaaatc
    17221 cagccaatcc ccagttcacg ggcgattagg gcgctgtggg ataccggact tccggcgcta
    17281 aggcagatac cttttacaac cgccggatcc agttgcagta ctgtggaagg ataaatgttc
    17341 tccgccagta gaatagttgg cgagttaaac tgcgggagtt cttctttcgt ttgggtcagg
    17401 tggaccaggg tgcgatgcag aagatcgtcc acatcaatat agcgagcttg tagatattca
    17461 tcatccagtt gctggtattg ctggctaagt tctttaagaa cttgctgcca ggcatattct
    17521 gccgtgcaat gttcatgctg aaggagttcg cttgccgccg ccagcagttc cggatcatct
    17581 aacagtgtat ggtgaccaga aaagattgcg gcaatatcgt caagcccgct ggcttctgct
    17641 ttcgctgtta acgtcatcag atctaataac gtgaagtcaa tagcctggcg taatcgatct
    17701 tgttcttctt ccacggtcag ggttgatttt gcctgtaccg tacataaaac tggttgataa
    17761 taaaaggctt tacccgaaac aggcggaacg ggacgcagag taggtggagc gacttcctcc
    17821 gtttcaccaa agttatcttc agccagctga cggaaagcga tcagtgcctc ttcagcttct
    17881 ggccctttcg caatcaggcg cagcgtatcg ttatagcgaa cttgtagtaa cgcaatctgg
    17941 ttaatactct ctggtgtgac gcatttgccg tttttttcca gcaacatatc ggcattaaat
    18001 gtcgataagg tataaaccag ccgggaggcc ggacgtacat gcaggccgtt acggtttttt
    18061 atgaccaccg ccagagaacg ggcttcttca tcgtacgcag gacatgtgtc agagatttca
    18121 gtgtcggagg acggtaaacc cagttgttca cgtttggctt ccagcgcatg catggcgtca
    18181 aagataactt tgtcgatatc cgcccccgag gccgcgctga ccgttgctgc cagtgtacct
    18241 tcgaccaacg gcgcagcaca caaacgtact tttgcggcga tctcgggagc cagcaattcc
    18301 agcgcagttt cagcactcaa taatgcgcta cccatatcca tcatgaccag cacatggtcg
    18361 gcatcagcaa cagattcgat ggcctccatc actttgacgg catcggtacc aatgggattt
    18421 tgtggatcgt caattcccgc ggcaatggcg attttacaac tatcactcat taacatctga
    18481 cgggctaatt caccgacacc ttctcccagt cggctgctat gtgaaactat gaccaggttt
    18541 accatcacca attccttact cttttgcggc taacgccaac atttgcatca taaacatcac
    18601 cgaggtcgcg ccgggatcct ggtgaccaat actgcgttca ccgagataac tggcgcggcc
    18661 tttgcgggct tgcatcgtaa tcgtactttg tgcagcggat tcggcgatgc tactggcagc
    18721 ttcgagcgcc accggaacag agagattttg ctcgctggac tgacgtaacg attccaccac
    18781 cggcacccac acatcacaca tggttttatc gccaggttcg gctttcccgc gactgattac
    18841 gccgtccgcg ccatcgcgga acatctgata aagctcttcc agtgtcaggc tttgccgtgc
    18901 ctgggtcgcc tgtgcggcgc ggataaagaa ggtaccgaac agcggaccac tggcaccacc
    18961 gacgctggaa agcagcgtca taccggtatt cttgagaatg aaaccgatat ctttatctgc
    19021 gatagcaggg agtttttcca ccactttgct aaagcctcgg ttcatattta gcccgtggtc
    19081 agcatcgcca atttcgcgat ccagtccggt aagatactcg ctctcggtgc tgaaaatatc
    19141 gccacaacga gtgagccagt taacaatttg agttctgctc agtgacattg ctttctcctt
    19201 atttacccca gttaagggcc ggggtgtgga ccggggcgtc ccagagtgcc agcgtttcgt
    19261 catcaacttt cagtaaggtg attgagaaac cggtcatatc cagtgaggtg cagtacgcgc
    19321 caattaaatt acgttcgata gtcaatcccg cttgctggca acgtgtggtc aggcggttat
    19381 agacgccgta cagctcagaa agcggagttg cgccaagatt gttaaccagc gcaatcaccc
    19441 gatcgccaga ctggagcggt tgtttggttt gttgttcttc ctgccaactg ccttgttgat
    19501 aatcccagaa acgcaaagtg cgatggtatg agccatttac cagcagggtg tcgaacattt
    19561 catcgacggt ttgatcaagg gaagagaagg ggcggcggtc aatacccggc tcaccatgaa
    19621 tgccgacgcc aaactccatc tcattatccg ccagggtaaa agaaggtttg cccgcggcag
    19681 gaacggtaca ggcaccgaga gcgataccta ttgagtggcc ttgattattc agcttacgcc
    19741 ccagttccgc acaggcgtcc agtgagtcgc cacgctccgc cgctgcgcct acgagttttt
    19801 caattaatac ggtgttggca acgccgcgtc gcccggcagt ataaagactg tcttttaccg
    19861 caacgtcgtc atcaatgacc acagtggtca cttttacgcc gctatcgtgc agtaactcgg
    19921 tcgctgtttc aaagttaaga atatcgccgg tgtaattttt gataatcaac agtacacctt
    19981 cgccgccatc aacttgcatg gcgcattcaa agattttatc gggcgtcggt gaggtgaaaa
    20041 tttcgcccgg acaggccccc gaaagcatcc cctgaccgat ataaccacag tgcatcggct
    20101 cgtgtccgct gccgccaccc gacagcaggg cgacttttcc tgcaacaggg gcatcagctc
    20161 gggtgacata caccggatcc tgatgcagtg tcagcgatgg atgcgctttc gccagtcctg
    20221 ccagttgttc gtccagtacg tcttgcacat cattgatcaa ttttttcatt attttgctcc
    20281 agcaattacg gtagggcatg gatgatgttc aacgacacgg cgaccactga ctgccgatga
    20341 atccattgtg catcaggcaa gggaaaagaa aattagccca aattatgttt catagtgaaa
    20401 catatgcttt aatgaatgtt ccatattgaa acttttacgt gtattaatac ttaaaattgc
    20461 gagccggaac accttttgtc ataagggatg cgggatatga gtggcgcttt taacaacgat
    20521 ggtcggggca tatctccctt aattgcaacc tcctgggagc gatgcaataa gctgatgaaa
    20581 cgggagacat ggaacgtacc acatcaggcc cagggcgtga catttgcttc tatttatcgg
    20641 cgtaagaaag cgatgctgac gctcgggcag gctgcgctgg aagatgcctg ggaatatatg
    20701 gcaccgcgag agtgtgcgct gtttatcctc gatgaaaccg cctgcattct cagccgtaat
    20761 ggcgatccgc aaaccttgca gcagctaagt gcactgggat tcaatgacgg cacgtattgc
    20821 gccgagggaa ttattggtac ttgtgcgcta tcgttagcgg ctatctctgg tcaggccgtg
    20881 aaaacgatgg ccgatcaaca tttcaaacag gcactctgga actgggcctt ttgtgcaacg
    20941 ccgttgtttg acagcaaggg ccgattgacg ggaacaatag cgctggcgtg tccggttgag
    21001 caaactaccg cagctgattt gccgttgacg ttggcaatcg cccgcgaggt cggaaattta
    21061 ctgctgacgg acagtttgct cgctgaaact aaccgtcatt taaatcaact taatgccctg
    21121 ttagaaagta tggatgatgg cgtgattagc tgggacgagc agggtaattt gcaatttatt
    21181 aatgcccagg cggcgcgggt cttgcgcctt gacgcgacgg caagtcaggg acgggcaatc
    21241 actgaactct taacgttacc cgccgtattg caacaagcaa taaaacaggc acatccgctc
    21301 aaacacgtag aagcaacctt tgaaagccag caccagttta ttgatgcggt gataaccctt
    21361 aaaccgataa tagaaacgca gggaaccagc tttattttgt tgctccatcc tgtggaacag
    21421 atgcggcagt tgatgaccag tcaattagga aaagtcagcc ataccttcgc tcatatgcca
    21481 caggacgatc cgcaaacccg ccgcttgatt cattttggtc gccaggcggc gcgcagtagc
    21541 tttcctgtcc tgctttgtgg agaagagggc gtgggcaagg cactgctaag tcaggcaatt
    21601 cataatgaaa gcgagcgtgc tgcaggtcct tatatcgccg tcaattgtga gttatatggt
    21661 gatgctgcgc tggcggaaga atttattggt ggcgatcgca cggacaatga aaatggccgt
    21721 ctgagtcggc tggaactggc acacggcggc acgctgtttc ttgaaaagat tgaatatctg
    21781 gcggtggagt tacagtctgc tttgcttcag gttatcaagc agggggttat cacgcgactg
    21841 gatgcgcggc gtttaatacc aattgatgtc aaagtgattg caacaacgac cgcggacctc
    21901 gcaatgctgg tggaacaaaa tcgttttagt cgccagctgt attacgcgct gcatgcattt
    21961 gaaattacca tcccgcctct gcgtatgcgg cgtggcagca ttccggcgct ggtgaataac
    22021 aaattacgca gtcttgaaaa acgcttctct acgcggctga aaattgatga cgatgccctc
    22081 gctcgcctgg tttcttgtgc atggccaggc aacgattttg aactttacag cgtcatcgag
    22141 aatcttgctc tgagtagtga taacgggcgc attcgcgtca gtgatttgcc ggaacatctg
    22201 tttaccgagc aggcgacaga tgatgtcagc gccacccgcc tttccaccag tctgtcattt
    22261 gcggaagttg aaaaagaggc aattattaac gcagcccagg tcacaggcgg tcgcattcag
    22321 gaaatgtcgg ctttacttgg gatcggccgc actacgctgt ggcggaaaat gaagcaacat
    22381 ggcattgatg cagggcagtt taagcgccgg gtatgaaaga cagaaacgat ttctgataca
    22441 tcagagtgat ctgtatttca ttccggcgca cgctaacaat tttcagcatc gtttaagggc
    22501 ttgtctatcc cgcacttaaa agctgaagcg atatcctccg ttgacttgct tctgatcaaa
    22561 gaggttaccc tgcgtgtaat ccgcttcgag atagaatgtg tgctgtttgt tatactgtgc
    22621 actgactccc acgccgttat tccagccatt acctttgaaa ctgtacttct cccgggagtt
    22681 gttcaacaga tattcggtat cccctgaaaa ctcgcggata gcgccagtct tcacatagac
    22741 attcagctgg ctgttgcctg cggtgatgtc ataccccagt atcatgctgg cacgccccag
    22801 cagcgattcg tagtgattca gatgtatatt gaggccatta ctcgccttca tagtcatctc
    22861 attctggtgg ctgtatataa gctgggtttg cggctctata tagaacccat aaccagtagg
    22921 ggacaggttg aacctctgcc cggcttccag ggagatgctc attccattcg cagtgccgtt
    22981 ggcgttaacg ccgttgttct gactgtccag tacgtggaaa ctatttttct ggcgcgatgc
    23041 ttttataacg agatcgctgt aaaaaccgtt ttgtgccatg taactggcgt acattcccat
    23101 gtagtctgaa cgtgcggtac cgtcgcctcc gctatagtcc ggcgatgcat gtgttgagcc
    23161 aatatacaga tcgacataca acggcattac atcagagaga cgtttatccc caccaaactg
    23221 gataccgctg taacccatgt caaagccgct cagtttgcca ctggcaaagg agtccaggct
    23281 tcccccataa ctgcgcaacc agatattacc gtctttactc tgatttcgca ggtcacccat
    23341 ccgttgcatc agcgtacggt tttcaacata gttcaataag taaccgacat tgagataatt
    23401 gccgccagca tctgcggtcg tggtgggttt aggcgtggga gcgggttcag gcgtaggatc
    23461 ggggttgact atgggaggct gagcgggagc tggtgtgggt tcaggattag gagtcggttc
    23521 cggaactgtc ccggaagcgt aaagctccca gttagtgcca tttttacgca catcgtacag
    23581 atatcccccc aactcaacct gagaagacgc gctgaacgag gccgtgccgt cagtggtttt
    23641 taccactgtc agaacttcat ttcccgttgt ggcctcgctg ccctggttgc ggatagccaa
    23701 tacgtgatta ccagcactac tcccgctgat attcaataaa tcccctttat tattaacgcc
    23761 attaccctcg ccaacaacat cagcacgcat aataaaggta ctgttaccgc tcaggttctc
    23821 tacgtttaat gtggcaaatg tgccggcagt tgacccgtgg ctggcaaaat cgacagttga
    23881 atggctcagc gccagcgtgt cgagattaga attacttgtt acgttccaga cgctgttatt
    23941 cattgcaacg tccagttttc cgccattgac attatcgctg agggaggaac ttgtccaaac
    24001 cgacccaggg tgcatatcca gattgattag cccacctttg gaaagaacgc taccgttgat
    24061 aagcatacga cccgaggcgt tgatgcggct ggcggcataa ccatcgtcat gttgcgttgc
    24121 gatggccatc tggtcgggtg tactcatatc aatgaccaga tcgctcgtga ggtcgatctg
    24181 gctgttggtc atggcataaa ttcccctggc tcctgccgcg ccggtgatag ccaaactgtc
    24241 tccggttatt ctaccgccgc tgagagccca caaacccagc gccagactgc cattacgatc
    24301 aactgtaata tcggtatttt gcatgttgac aacagccgtt gccgtctggg ccgaggcacc
    24361 ataagaaccg ccggaaaaga tgctgtttcg ttgcgctgcc gtgccagtaa aattgattat
    24421 cgcgtctgaa ccactggtga cgaggccacc gccctgcgcg gaagaaatat ggctatctgc
    24481 accgatagtg gttgtaccac cacgaacttc gacgccattg gccgcggctc cagttacatc
    24541 aacagtgagt gcattcgcgc taacctggcc aaagctccag aggccgtgag cattatcgcc
    24601 attggtttta atggtactgt ttgttccgag atcgacaaca gagtttttct gtacgtttat
    24661 ccccatggcg ctgtagccct gaacatcgat tgtcaggtct gtagccgtaa aacgcgcagc
    24721 accattggcg ttattgccgt tgagaccacc gatataaaca cctgtacttc catcggtcgt
    24781 gattttactt ccgcttccaa gatcgacact ggtgccatag tcattgatgg ttaggcctat
    24841 accgttcgag ttttcaatgg tgaattgagt ggctgttagt gttgagctat gcccaataat
    24901 gatgccgtca tcgttactct taatggtgct gcctgtgcct aagtcagcat gggtatagtc
    24961 accaattaag ttaatgccga tggcagaggt ttgaccaaca acatctactg tgagtcggtt
    25021 agctgtcagg gagctgtttt tgccctgaat aatgattcct tttgcatatc cagaggcgtc
    25081 gttgacattg acggtgacat cattacccag gttgatattg ccaggcgtgt ttcctgctgg
    25141 ggtcattaca ctatagaggt ttcctgatgg atcggcggta tctcctgtaa ttctgtcacc
    25201 atcattaata gtaatattgg tgccatattg acttcctgta tcagcagcta tcagcagggg
    25261 ggaaaatata attgcattca aaccaatagc gccaaatttt atcagttgaa ttgagggcga
    25321 acggattttt aattcagcga gacgatctgc tttggtatt
//
LOCUS       DAPHBJ010000053        24914 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.053, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000053 DAPHBJ010000000
VERSION     DAPHBJ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.053"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(91..174)
                     /gene="yohP"
                     /locus_tag="RHZ76_001906"
     CDS             complement(91..174)
                     /gene="yohP"
                     /locus_tag="RHZ76_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HDV2812558.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            398..1834
                     /gene="mdtQ"
                     /locus_tag="RHZ76_001907"
     CDS             398..1834
                     /gene="mdtQ"
                     /locus_tag="RHZ76_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HDV2812559.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNNVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSAIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            1887..2648
                     /locus_tag="RHZ76_001908"
     CDS             1887..2648
                     /locus_tag="RHZ76_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV2812560.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(2778..3356)
                     /locus_tag="RHZ76_001909"
     CDS             complement(2778..3356)
                     /locus_tag="RHZ76_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV2812561.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            3526..4113
                     /locus_tag="RHZ76_001910"
     CDS             3526..4113
                     /locus_tag="RHZ76_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HDV2812562.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            4287..5219
                     /gene="pbpG"
                     /locus_tag="RHZ76_001911"
     CDS             4287..5219
                     /gene="pbpG"
                     /locus_tag="RHZ76_001911"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HDV2812563.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(5257..6972)
                     /gene="dld"
                     /locus_tag="RHZ76_001912"
     CDS             complement(5257..6972)
                     /gene="dld"
                     /locus_tag="RHZ76_001912"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDV2812564.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            7168..9465
                     /gene="bglX"
                     /locus_tag="RHZ76_001913"
     CDS             7168..9465
                     /gene="bglX"
                     /locus_tag="RHZ76_001913"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HDV2812565.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            9717..10634
                     /gene="osmF"
                     /locus_tag="RHZ76_001914"
     CDS             9717..10634
                     /gene="osmF"
                     /locus_tag="RHZ76_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HDV2812566.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLHAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGATPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSLYLKEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            10641..11798
                     /gene="yehY"
                     /locus_tag="RHZ76_001915"
     CDS             10641..11798
                     /gene="yehY"
                     /locus_tag="RHZ76_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HDV2812567.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGARRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            11791..12717
                     /gene="yehX"
                     /locus_tag="RHZ76_001916"
     CDS             11791..12717
                     /gene="yehX"
                     /locus_tag="RHZ76_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HDV2812568.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERADGEALAEEMTLRDALS
                     LFVARGCEVLPVVNMQGQPCGTLHFQDLLVEA"
     gene            12722..13453
                     /gene="yehW"
                     /locus_tag="RHZ76_001917"
     CDS             12722..13453
                     /gene="yehW"
                     /locus_tag="RHZ76_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HDV2812569.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(13434..13541)
                     /locus_tag="RHZ76_001918"
     CDS             complement(13434..13541)
                     /locus_tag="RHZ76_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HDV2812570.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(13601..14332)
                     /gene="mlrA"
                     /locus_tag="RHZ76_001919"
                     /pseudo
     CDS             complement(13601..14332)
                     /gene="mlrA"
                     /locus_tag="RHZ76_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
     gene            14554..16239
                     /gene="btsS"
                     /locus_tag="RHZ76_001920"
     CDS             14554..16239
                     /gene="btsS"
                     /locus_tag="RHZ76_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HDV2812571.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            16236..16955
                     /gene="yehT"
                     /locus_tag="RHZ76_001921"
     CDS             16236..16955
                     /gene="yehT"
                     /locus_tag="RHZ76_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HDV2812572.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            17002..17472
                     /locus_tag="RHZ76_001922"
     CDS             17002..17472
                     /locus_tag="RHZ76_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDV2812573.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(17513..17974)
                     /locus_tag="RHZ76_001923"
     CDS             complement(17513..17974)
                     /locus_tag="RHZ76_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HDV2812574.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAARALEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(18099..20102)
                     /locus_tag="RHZ76_001924"
                     /pseudo
     CDS             complement(18099..20102)
                     /locus_tag="RHZ76_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
     gene            complement(20099..21235)
                     /locus_tag="RHZ76_001925"
     CDS             complement(20099..21235)
                     /locus_tag="RHZ76_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDV2812575.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLATARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(21228..23507)
                     /locus_tag="RHZ76_001926"
     CDS             complement(21228..23507)
                     /locus_tag="RHZ76_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812576.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQIALPSALAHYFA
                     QLRGDSPGDVLNRQREAFMTRWIGWAMQQNDGDVLVVCGGWHAPALAKMWRECPQEIN
                     KPELPSLADAVTDCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQKAGEQLLKT
                     ILTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDALAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEEHWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDTGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DDDFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(23518..24606)
                     /locus_tag="RHZ76_001927"
     CDS             complement(23518..24606)
                     /locus_tag="RHZ76_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDV2812577.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAWLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         5830 a         6351 c         6772 g         5961 t
ORIGIN      
        1 ttatatgaag caatgcacat gacaggagcg caacaggatg agagactgaa atattctcgt
       61 tattttcgga cctgatgaca ttaattaatt ttaaaatatc atcttaaata cgccagtcac
      121 caccagtagc ccaatcagga aaataatcaa tacagcccag agtataattt tcattggcgt
      181 tcctttattt gtgagtgctg aataagtata ggaaaaaacg aagccctttg ccgttatcca
      241 taaacgatta agataaatcg gaagatatca tttacttagt ctatattttt ctgggtatgt
      301 cgttttattg ctactgatgt attgccacgc gaaactaaaa aatatatcgc aacacacggt
      361 aatctccgcg caccgtttct tacctgatta ttcccccatg aatcgtgatt ccttttatcc
      421 agccatcgcc tgttttccgc tgttactgat gctggccggg tgtgcgccta tgcatgaaac
      481 ccgccaggcg ttaagccagc aaacgcccgc tgcacaagtt gacaccgcat tacccacggc
      541 gctgaaaaat ggttggccag acagccaatg gtggctggag tatcacgata atcaactcac
      601 ttcattaatt aacaatgcac tacagaacgc accggatatg caggtggctg aacagcgcat
      661 tcagctagct gaagcccagg cgaaggcagt tgccactcag gatggtccgc agatcgactt
      721 ttcggcggat atggagcggc aaaaaatgtc ggcagaaggc ttaatggggc cgtttgctct
      781 gaacgatccg gccgcaggta cgaccggccc gtggtacacc aacggtactt ttggcttaac
      841 ggcgggctgg catctcgata tctggggaaa gaatcgggcg gaggttactg cccgcctggg
      901 tacggttaaa gcacgggcgg cggaacgcga gcaaacccgc caattgctgg ctggcagcgt
      961 agcccgcctg tactgggagt ggcaaaccca ggcggcgtta aacacggtct tgcagcaaat
     1021 agaaaaagag cagaacacca ttatcgcgac cgatcgccag ctatatcaga acgggattac
     1081 ttcttcagtt gaaggtgtgg aaaccgatat taatgccagc aaaacccggc agcagctcaa
     1141 caatgtcgcg gggaaaatga aaattattga ggcacggtta agcgcactta caaataacca
     1201 gacaaagtca ttgaagctta aaccggtcgc gttgccgaaa gtggcaagcc agcttcctga
     1261 tgaactgggg tactccttac tggcccggcg ggcagatttg caggcggcgc actggtacgt
     1321 tgagtcatcg ctaagcgcca ttgatgcggc aaaagcggca ttttatcctg acatcaacct
     1381 gatggccttc ctgcaacagg atgcgttgca cttaagcgat ctgttccgtc attccgcgca
     1441 gcaaatgggc gttacggcag gcctgacgct acccattttc gatagtggtc gtcttaacgc
     1501 caatctcgat atcgcaaaag ccgaaagcaa cttgtctatc gccagctaca acaaagcggt
     1561 ggttgaagcg gtgaatgacg tggcgcgggc agccagtcag gttcagacac ttgcggagaa
     1621 aaaccagcat caggcgcaaa ttgagcgcga tgccttgcgt gtggtaggtc ttgcgcaggc
     1681 gcgctttaac gcgggcatca ttgctggttc ccgcgtcagc gaagccagaa tccccgcgct
     1741 gcgtgagcgg gccaatggcc tgttattgca agggcagtgg ctggatgctt ccattcaact
     1801 cactggtgcg ttgggcgggg gttacaaacg ctgatgatat gcgccttcta tacttaacgt
     1861 ttattcagcg ttaagtggag aactcgatgg cacaggttgc gattattacc gcctccgatt
     1921 cggggatcgg caaagagtgc gcgttattac tggcgcagca ggggtttgat attggcatta
     1981 cctggcactc agatgaagaa ggggcaaaag ataccgcgcg tgaggtagtt agccacggcg
     2041 tacgtgcgga gatcgtgcag ctggatctcg gtaaactgcc agaaggtgcg caggcgctgg
     2101 agaaactcat tcaacggctg gggcgtattg atgtgctggt gaataatgcg ggcgcaatga
     2161 ccaaagcgcc gtttcttgat atggctttcg atgagtggcg caagattttt accgttgatg
     2221 tcgatggtgc atttttatgc tcacaaattg cggcccgcca gatggtgaaa caagggcagg
     2281 gcggtcgcat catcaacatt acgtcggtac atgaacatac gccgctgccg gatgccagcg
     2341 cttacacagc cgctaaacat gcgctcggcg ggttaaccaa aacgatggcg ctggagctgg
     2401 tcaggcataa gattttggtg aacgcagtcg cgcctggggc gatcgccacg ccaatgaatg
     2461 gcatggatga cagcgacgtg aagcccgacg cggagccttc gattcctctg cggcgttttg
     2521 gcgcaacgca tgagattgcc agcctggtag cgtggctgtg ctcagagggc gcaaattaca
     2581 ccaccgggca gtcgttgata gtggatggcg gctttatgtt ggcgaatccg cagttcaacc
     2641 cagaatagcg cagtgaggtg cgtggttatt tcaatgggta aaattttggt aggcatgata
     2701 agacgcacaa gcgtcgcatc aggcatggtg caccggatgt cggatgcggc gcgaacgcct
     2761 tacccagggc aatggtttta cgcctgatga tccggctttt tcttcccgcg ccgtttcagc
     2821 caccagcgca cgcccaccac cagaaccacg accagaatca accagaccca gtgcttcaaa
     2881 tgctggtcga gattgtgcaa ccacggcgca atcacctgac caccagcgta accaatagtg
     2941 gtaaaaatca acgcccaggc aaatgcgccg agaatattca gcggcagaaa gattttcggc
     3001 ggcagctggc tggcaccaat cagcgtcggg ccaatcaccc gaaagccata cataaagcgc
     3061 gtaccaatga caaacagata cggatggcgt tggataagtt tctgcgcccg ctcaatttta
     3121 tcctgatgtt tcgagaaacg gcgtaacagc ttgccgccaa accgccgccc gcacagataa
     3181 agcacctggt cgccaatcat gccgccaagc gccacagaaa gtaccaccag cgggaacttt
     3241 aatagcccct gatgtgccgc aacgcctccc agcaaagtca cggtttcacc ttccgccagg
     3301 ctaccgatca ccagcgcggc ataaccatat tgtgagatga gtgtattgag atccataggc
     3361 cagtctgtct ccctttggtt gcctttaagc atacaccctg gaaatcattc acgctgaaaa
     3421 aggacgtaca tcagacggtg caaattacat gagcgaacaa aaaataatca caagctgact
     3481 tatacttgaa ctgtcaggtt catgaactca caacaagggg gcggtatgag ccatgtctgg
     3541 ggactgtttt cccatcccga tcgtgaaatg caggtgatta atcgcgaaaa cgaaacgatt
     3601 tctcaccatt acacccacca cgttttgctg atggcggcga ttccggtgat ttgcgccttc
     3661 attggcacta cacagattgg ctggaatttt ggcgatggca ctatcctgaa gttatcctgg
     3721 tttaccggac tggcgctggc tgtcctgttt tatggcgtta tgctggctgg ggttgcggta
     3781 atggggcggg tcatctggtg gatggcgcgt aattatccgc agcgtccgtc acttgcgcac
     3841 tgcatggtct tcgcgggcta cgttgcaact ccgctgtttt taagcggtct ggtggcgctt
     3901 tatccactgg tctggttgtg tgcgctggtc ggcacggtgg cgctgtttta caccggctac
     3961 ctgctttacc ttggtattcc gagcttcctg aatatcaaca aagaggaagg ccttagcttc
     4021 tctagttcga cgctggcgat tggcgtgctg gtactggaag tgctgctcgc cctaaccgtt
     4081 atactatggg gctacggtta tcgtttgttc tagaaagttc tttgacgttg cattgctggc
     4141 gtaaatgtca gcaatgcagc atttcctcac gattctcctt tgacgatcgc actttttgct
     4201 cgttatgatg gcgtcgctag cctcagtaaa tccttactcc ggcggtgcgc aacccgtgcg
     4261 cgtgaaccac tatctgaatg ctcatcatgc cgaaatttcg agtttcttta tttagcctgg
     4321 ccctgatgct ggctgtgcct ttggcaccgc aggccgttgc gaaaacggca gccgctacca
     4381 ccgcttcaca accggaaatt gcctccggta gcgcgatgat tgttgatctg aataccaaca
     4441 aagtgatcta ttcgaaccac ccggatctgg tgcgtccgat tgcgtctatc agcaaattaa
     4501 tgaccgcgat ggttgtgctg gatgcacgac tgccgctgga tgaaaaacta aaagtggata
     4561 tcagccagac gccggagatg aaaggggtct attcgcgcgt acgactgaat agcgaaatca
     4621 gccgtaaaga tatgctgttg ctggcgctga tgtcttcaga aaaccgcgcg gcggcaagcc
     4681 ttgcgcacca ttatcccggt ggttacaaag cctttattaa ggcaatgaat gcgaaagcga
     4741 aatcgctcgg aatgaacaac acgcgctttg ttgaacctac cggattgtcg gtgcataacg
     4801 tttcaactgc ccgtgactta accaaactgc tcattgccag caaacaatat ccgttgatcg
     4861 ggcagttaag tactacccgg gaagatatgg caaccttctc taacccgacg tatacgctgc
     4921 cgttccgcaa tactaatcat ctggtgtatc gcgataactg gaatattcag ttaaccaaaa
     4981 ccggctttac caatgcggcg ggccattgtc tggtgatgcg tacggttatc aataataaac
     5041 cggtggcgct ggtagtgatg gacgcgtttg gcaaatatac ccattttgcc gatgccagcc
     5101 gcctgcgtac ctggattgaa accggtaaag tgatgcctgt gccggcagca gcgttgagct
     5161 ataaaaaaca aaaagccgcc caaatggcgg cggcggggca gacggcacag aacgattaag
     5221 tgaattcgga tggcgatact ctgccatccg taatttttac tccacttcct gccagttttt
     5281 ccgtttactg gttttaccga tccccggatt catgctgttg gtcggatcgt tctcgcgata
     5341 gaacttctgc aacgtctccg gtgctttata caaatgaccg acgttatgct cggcagggta
     5401 ctgcgcgccg cgctgctgta gcagttccag catctgttct tttaacgcat gcacatccac
     5461 gccttttttc actatgtaat cctgatggaa gacatagcac ataaaatggc cgtaatagag
     5521 cttgtgcacc agctggctgt cgatctccgg cggtaaatgc tcataccact cggtgtcgtt
     5581 acgccgcaga gcgatatcca acgccagaat gtcttcgact tcatcggaat gcaccgcctg
     5641 ataacgaatt gctgcgcccg cagcggcgaa acggtgtaaa aacgctttgc tgccttcctc
     5701 cggcgtacag acaaagaaat cgccttcggc ctgtttgaaa taatctacca gccacgattt
     5761 ggcttcaccg acgccatcgc ccgccatttt taacagcaga tgatgctcgt atttatcgcg
     5821 ccagtttttc atgcgcggcg gtaaatggct ggggaacagg tgaccgaatt tttgcatcgc
     5881 acggtcagta aaatgcggac ggaagaattt cactttctcc agcatcgcat cggtgcgtcc
     5941 cttgagatta aagaagaacg gcatcttgtc ggtgcctaac ttatcaatca tcaggaaggt
     6001 gtctttgccg tatttttccg caatatcgta gatatcccgg tgcatatatt ccccggcaac
     6061 cggcagattt tcaaagttag ccagaatatg gcggcggatt tcggtcagca cttccggctg
     6121 gttggtgccg atataaaaca cctgctgatt tttttccgct tcgaaggtat ctagacgtac
     6181 cgcaaagacg gccagcttcc cggcgcaacc agaagattca aataaccgat caggatcggc
     6241 gttataacgt gcgggcgtgt cggcttcaat atcacgaacg cggtggacat agtcataatc
     6301 gtgggcgtga cgaccatcgt gacgcacatc gtcatctttg atgcgatcat catccagctt
     6361 gctaaggatc tgctccggcg tttcgcccag atcaatcccc agatggttca ctagcgtcag
     6421 tttgccgtct tcatttatac gcgcgaataa cgacatttcg gtatacgccg ggccacgttg
     6481 caccagcgag ccgccggagt tgttacaaat accgccgatg accgatgcgc ctatacacga
     6541 tgatccaatc actgagtgcg gttcgcgtcc cagcggtttg agggcttttt ccagcgaata
     6601 gagcgtggtg cccggatagg ccagcacctg ttcgcccttg ccaagaacgt gcagcttgtc
     6661 gagacgcagg gtgctgatga tcacgatatc gcgatcatag tcgttaccgt ttggcgtcga
     6721 tccttcggtc aggcctgtat tggcagcctg catcagaata attttgtcgg cggtgacaca
     6781 ggctttcagc acccgccaca attccagtag tgagccaggg aaaacgacag ccagcgcgtc
     6841 gccctgacca gaacggaagc ccttgcgata gcgggccgtt tttgcgggat cggtgagcag
     6901 gtgtgaatga ccgaccagac gggcaagttc attcaaaaag gctttattat cagttgttgt
     6961 catggaagac attttccact ccttgtggtg gcgaaaaaat atcacaaact agaatagcgc
     7021 cttgtatgac aattcagcga gtgaatattt tgaaaattca gacaataaac tgagcattct
     7081 ttttctctct ccatcatgct tatggcaaac tacggttttt gcaccatttt cgctgcctcg
     7141 tcgggcaaca gaggaagaaa aatccatatg aaatggctat gttcagtagg aatcgcggtg
     7201 agtctggccc tgcagccagc actggcggat gatttattcg gcaaccatcc attaacgccc
     7261 gaagcgcggg atgcgttcgt caccgaactg cttaagaaaa tgacagttga tgagaaaatt
     7321 ggtcagctgc gcttaatcag cgtcggcccg gataacccga aagaggcgat ccgcgagatg
     7381 atcaaagacg gtcaggttgg ggcgattttc aacaccgtaa cccgtcagga tatccgcgcc
     7441 atgcaggatc aggtgatgga attaagccgc ctgaaaattc ctcttttctt tgcttacgac
     7501 gtgctgcacg gtcagcgcac ggtgttcccg attagcctcg gtctggcctc gtcttttaac
     7561 ctcgatgcag tgaaaacggt cggacgtgtc tctgcttatg aagcggcaga tgatggcctg
     7621 aatatgacct gggcaccgat ggtcgatgtc tcgcgcgatc cgcgctgggg acgtgcttcc
     7681 gaaggttttg gcgaagatac gtatctcacc tcaacaatgg gtaaaaccat ggtggaagcg
     7741 atgcagggta aaagcccggc agatcgctac tcggtgatga ccagcgtcaa acactttgcc
     7801 gcatacggcg cggtagaagg cggtaaagag tacaacaccg tcgatatgag tccgcagcgc
     7861 ctgtttaatg attatatgcc gccgtacaaa gcggggctgg acgcaggcag cggcgcggtg
     7921 atggtggcgc tgaactcgct gaacggcacg ccagccacct ccgattcctg gctgctgaaa
     7981 gatgttctgc gcgaccagtg gggctttaaa ggcatcaccg tttccgatca cggtgcaatc
     8041 aaagagctga ttaaacatgg cacggcggca gacccggaag atgcggtgcg cgtggcgctg
     8101 aaatccggaa tcaacatgag tatgagcgac gagtactact cgaagtatct gcctgggttg
     8161 attaaatccg gcaaagtgac catggcagag ctggacgatg ctgcccgcca tgtactgaac
     8221 gttaaatatg atatggggtt gtttaacgac ccatacagcc atttggggcc gaaagagtct
     8281 gacccggtgg ataccaatgc cgaaagccgc ctgcaccgta aagaagcgcg tgaagtggcg
     8341 cgcgaaagcc tggtgttgct gaaaaaccgt ctcgaaacgt taccgctgaa aaaatcggcc
     8401 accattgcgg tggttgggcc actggcggac agtaaacgtg acgtgatggg cagctggtcc
     8461 gcagccggtg ttgccgatca atccgtgacc gtactgaccg ggattaaaaa tgccgtcggt
     8521 gaaaacggta aagtgctgta cgccaaaggg gcgaacgtca ccagtgacaa aggcattatc
     8581 gatttcctga atcagtatga agaagcagtc aaagtcgatc cgcgctcgcc gcaagagatg
     8641 attgatgaag cggtgcagac cgcgaaacaa tctgatgtgg tggtggctgt ggtcggtgaa
     8701 gcacagggga tggcgcacga agcctccagc cggaccgata tcactattcc gcaaagccaa
     8761 cgtgacttga ttgcggcgct gaaagccacc ggtaaaccgc tggtgctggt gctgatgaac
     8821 gggcgtccgc tggcgctggt gaaagaagat cagcaggctg atgcgattct ggaaacctgg
     8881 tttgcgggga ctgaaggcgg taatgcaatt gccgatgtat tgtttggcga ttacaacccg
     8941 tccggcaagc tgccgatgtc cttcccgcgt tctgtcgggc agatcccggt gtactacagc
     9001 catctgaata ccgggcgtcc gtataatgcc gacaagccga acaaatacac ttcgcgttat
     9061 tttgatgaag ctaacggggc gctttatccg ttcggctatg gtctgagcta taccactttc
     9121 accgtctctg atgtgaaact ttctgcgccg accatgaagc gtgacggcaa agtgacggcc
     9181 agcgtgcagg tgacgaacac cggtaagcgc gaaggggcca cggtagtgca gatgtacttg
     9241 caggatgtga cggcttctat gagccgcccg gtgaagcagc tgaaaggctt tgagaaaatc
     9301 accctgaagc cgggcgaaac tcagaccgtc agcttcccga ttgatattga ggcgctgaag
     9361 ttctggaatc aacagatgaa atatgacgcc gagcctggca agttcaatgt ctttatcggc
     9421 actgattccg cacgcgttaa gaaaggcgag tttgagttgc tgtaatgctt tagtaagggc
     9481 gcgacgtttg cgccctttgc ctgatgttca tatcttaact tgctgaattt tcgccctttg
     9541 taggccggat aaggcgttta cgccgcatcc ggcaagaaac gccgggcaaa atatgccctt
     9601 taagtattta tctgattctg caactgaatc cttccgctca ggctaactct gtcattatca
     9661 actatgcttt tctcttaatt cgctgaaaac aacagtaaaa gaatgaggaa agcatcatgc
     9721 cactctcaaa ggtctgggca ggttcactgg ttttgttggc agccgtgagc ctgccgctgc
     9781 acgcggcttc ccccgttaaa gtcggttcaa aaatcgatac cgaaggcgcg ctgctcggca
     9841 atatcatttt gcaagtactc gaaagccacg gagtaccaac ggtcaataaa gtgcaactcg
     9901 gagcgactcc cgtggtgcgc ggggcgatta cttccggtga actggatatc tatccggaat
     9961 ataccggcaa tggcgctttt ttctttaaag atgaaaacga tgcggcatgg aaaaacgcgc
    10021 agcaaggtta cgagaaagtc aaaaaactcg atgcagagca aaacaagtta atctggctga
    10081 cgcccgcacc agcgaataac acctggacca tcgccgtgcg tcaggatgtg gcagagaaaa
    10141 acaaactcac ttcgcttgct gacctgagtc tttatctgaa agagggcggc accttcaaac
    10201 tggcagcctc ggcagagttt atcgaacgcg ccgatgcgtt acccgcgttt gaaaaagcct
    10261 atggctttaa gctcggtcag gatcagttgc tgtcactggc tggtggcgac acggcggtga
    10321 cgatcaaagc cgctgcccag caaacctctg gcgttaatgc tgcaatggct tacggcaccg
    10381 acggcccggt agcggcgctg gggctgcaaa ccttaagcga tccgcaaggt gtgcaaccta
    10441 tctacgcgcc tgcaccagtg gtgcgtgagt cggtgttgaa ggaatatccg caaatggcac
    10501 agtggctaca gccagtcttc gccagcctcg atgcaaaaac attgcagcag ctgaatgcca
    10561 gcattgcagt ggaaggactg gatgccaaaa aagtggctgc cgactacctg aaacaaaaag
    10621 ggtggacgaa gtaatttccc gtgacttatc tacgtattaa tcctgttctg gcgctgctgc
    10681 tgttgctgac ggcaatcgca gcagcgctgc cgtttatcag ctacgcgcct aatcgtttag
    10741 tctcgggtga agggcgacat ctctggcagc tgtggccgca aacgatttgg atgctggtgg
    10801 gcgttggttg cgcctggctg acagcctgtt ttattcccgc taaaaaaggc agcatttttg
    10861 cactcattct ggcgcaattc gtcttcgtat tgctggtgtg gggagcagga aaggcggcga
    10921 cccaactggc gcaaaatggc agtgcgctgg cgcgtaccag cctcggcagt ggtttctggc
    10981 tggccgcggc gctggcattg ctggcctgta gtgatgccat ccgccgaatc tccacgcatc
    11041 cgctgtggcg ctggttgttg catatgcaga ttgccattat tccgctgtgg ttgctgtact
    11101 ccggcacgct taacgatctc tcactaatga aagaatacgc caaccgtcag gatgtgtttg
    11161 acgacgcgct ggcacaacat ctgacgttgc tgtttggtgc ggtgctgcct gcgttagtga
    11221 ttggtgtgcc gttgggcatc tggtgctact tttccactgc tcggcagggg gcaatttttt
    11281 ctctgctcaa tgtcattcag accgtgcctt cggtggcgct ctttggcctg ttgattgcgc
    11341 cgcttgccgc gctggtgacg gcctttccgt ggctggggaa gctcggcata gcaggaaccg
    11401 gaatgacacc cgcactgatt gcgctggtgc tctatgcctt gctgccgctg gtgcgcggcg
    11461 tggtagtcgg cttgaaccag atcccgcgcg atgtgctgga gagcgccaga gcgatgggca
    11521 tgagcggggc gcggcgattc ctgcatgttc agttaccact ggcgttaccg gtatttctgc
    11581 gcagcctgcg ggtggtgatg gtgcaaactg taggtatggc ggtgattgcg gcgttaatcg
    11641 gcgcaggcgg ttttggtgcg ctggttttcc aggggctgct aagcagcgcc attgatttag
    11701 tgttgctggg ggtgatcccg gtaattgttc tggcggtgct taccgacgcg ctgttcgatt
    11761 tgcttatcgc actgctgaag gtgaaacgta atgattgaat ttagccatgt cagcaaactg
    11821 ttcggcgcac aaaaagccgt taacgatctc aatctcaatt ttcaggaagg gagtttttcg
    11881 gtgctgattg gcacatctgg ctccggcaaa tccaccaccc tgaaaatgat taaccgcctg
    11941 gtggagcatg acagcggagt gatccgcttt gccggagaag aaattcgctc gctgccagta
    12001 ctggagttgc gccgccggat gggctatgcc attcaatcta ttggcctgtt cccccactgg
    12061 agcgtggcgc aaaacatcgc taccgtgccg caattacaaa aatggtcgcg ggcgcggatt
    12121 gacgatcgta tcgacgaatt aatggcgcta ctggggctgg agtcaaattt gcgtgagcgt
    12181 tatccgcatc agctttccgg tggtcagcag caacgtgtgg gagtggcgcg tgcactggct
    12241 gccgatccgc aagtcttact gatggatgaa ccttttggcg cactggaccc ggtaacgcgc
    12301 ggcgcgttgc aacaagagat gacgcgcatt caccgtttgc tggggcgtac cattgtgctg
    12361 gtcactcatg atattgatga ggcgctacgg ctggcagaac atctggtatt gatggatcac
    12421 ggtgaagtag tgcagcaggg caatccgctg acgatgctga ctcgtccggc gaatgatttt
    12481 gtccgccagt tttttggacg tagtgaactg ggtgtgcgcc tgctttcgtt acgtagtgtg
    12541 gcggattacg tgcgtcgcga agaacgagca gatggtgagg cactggcaga agagatgacg
    12601 ctacgcgatg cgctctctct gtttgttgcg cggggatgcg aggtgctgcc ggtggtgaac
    12661 atgcagggcc agccttgcgg cacgctgcat tttcaggatc tgctggtgga ggcgtaagcg
    12721 tatgaagatg ttgcgcgatc cgctgttctg gctcattgct ttgtttgtgg cactgatttt
    12781 ctggctgcct tacagccagc cgctgtttgc tgccttgttc ccacaactgc cacgacccgt
    12841 ttatcagcaa gaaagttttg cagctctggc actggctcat ttctggctgg tgggaatttc
    12901 gagtttgttt gcggtgatca ttggcactgg tgccggaatt gcagtcactc gcccgtgggg
    12961 cgcggaattt cgcccactgg tggaaactat tgccgccgtt gggcagactt ttccgcctgt
    13021 cgcagtgctg gcgattgccg ttccggtgat cggctttggt ctgcaaccag cgattatcgc
    13081 cttgatcctt tacggtgtgc tgcccgtcct gcaggcgaca cttgccgggc tgggagcgat
    13141 tgatgccagc gtgacagaag ttgcgaaagg tatgggaatg agtcgtggtc agcgactgcg
    13201 taaggtcgag ctaccgctgg cggctccggt gattctggcg ggcgtgcgaa cttcggtgat
    13261 tatcaacatt ggtacggcga cgatcgcctc aacggtaggg gccagcacgc tgggtacgcc
    13321 catcatcatc gggcttagcg gatttaatac cgcgtatgtg atccaggggg cgttactggt
    13381 ggcactggcg gcgatcatcg cagaccgcct gtttgaaagg ctggtgcagg cgcttagcca
    13441 gcacgcaaaa taaaggtata acctgcgagc atgacgccac caattccgcc taacgccata
    13501 aacaggaaca gggcgatgac cccaatttta gctatgcgca taatgcactc cttatgttaa
    13561 cgaaaggatt gtacagtaaa gcgcatttgt taacgaatca ttaaatgccg agtgggaaaa
    13621 tatcatggcc ttgttcctgc caactggtga gttgttgctg ttgggcggag gttcgatttt
    13681 caccgcacca caccagcaat gtacggcctt cgaatagttc agggcgtagt tgattgagcg
    13741 agtgggcgag gacatcaatg cgccatcctt gttgactggc aatccagccc tccagccaca
    13801 gacgggtggt atcctgaata ttccagccaa ccaccagcgc atctttaccc tgttttttac
    13861 gtgccgaagc cagacaaatg gcgatgtagt tgatcagtac gccgtcgagg atcgccagca
    13921 gcgcctggag agtcggttgt tggcactgaa gccgtcggcg cagaggaata aacagatgtg
    13981 tggtgagtgt ctgggcgggg taatcctgac cgcgctcttt gatccacgtt cgcaggctat
    14041 gtagattgcc gctttgcagg taagtcagta atgtttcttg ctgatcgcgc cagccgttct
    14101 gcacatcaac attttcatta ctgagcagca ttttaacttt gctgacctgc acgccgttgt
    14161 cgatccagcg tttgatctcg cggatccggt caatatcggc atcgttgaac agccgatgac
    14221 cgccgtctgt ccgttgcggt ttcagcaatc cgtaacgcct ctgctacgcg cgtaacgtga
    14281 caggattaat atcacaaagc aacgccactt caccaattgt gtaaagcgcc atcgtctcac
    14341 ccttgctcgc gaggtcccag tttaacttta gacgcagttt tgcgaaccag gtagttttgc
    14401 ccgttttttg tgcatctata gggtgatttt atttttgcca ggcgattttg agtgatcgta
    14461 ctcacgaatt ctcatttttc tgcaagagtt caaagaaagt taaacgcagg caatgtatgt
    14521 tacgcgtttt aaagggaagt gtggtttgcg ggtatgtacg attttaatct ggtgttgctg
    14581 ctgcttcagc agatgtgcgt ttttttagtc attgcgtggt taatgagtaa aacgccatta
    14641 ttcataccgt taatgcaggt cacggttcgt ctgccgcata aatttctctg ctacatcgtc
    14701 ttttccatct tctgcatcat gggcacctgg tttgggttgc acattgacga ttctattgcc
    14761 aatacccgtg cgataggcgc ggtaatgggc ggcttactcg gcggtccggt cgtcggtggg
    14821 ctggttggtc tgaccggcgg cttacatcga tattcgatgg ggggcatgac cgcgctaagt
    14881 tgcatgatct caaccatcgt tgaaggattg ctcggcggcc tggtacacag catcctgatc
    14941 cgtcgcgggc gcactgataa agtctttaac cccattaccg ccggtgccgt cacgttcgtc
    15001 gctgaaatgg tgcaaatgct gatcatcctt gcgatcgccc gaccttatga agatgcggtg
    15061 cgtctggtga gtaatattgc tgcgccaatg atggtcacca ataccgtcgg cgcggcgctg
    15121 tttatgcgta tattgctcga taaacgcgcg atgtttgaaa aatacacttc ggctttttct
    15181 gccactgcgc tgaaagtggc agcctcgacg gaaggcattt tgcgacaggg gtttaacgaa
    15241 gtgaacagca tgaaagtggc tcaggtgctg tatcaggagc tggatattgg tgcagtcgcg
    15301 attaccgatc gagagaaatt gctggccttt accggaattg gtgacgacca ccatttaccc
    15361 ggcaaaccga tttcttcgac ttacacctta aaagcgattg aaaccggtga agtggtctac
    15421 gctgatggca acgaagtacc ttatcgttgc tctttgcatc cgcaatgcaa actggggtcg
    15481 acgctggtaa ttccgttgcg tggcgaaaat cagcgcgtga tgggcaccat caaattgtat
    15541 gaagccaaaa accgtttatt cagttcaata aaccgcacgc tgggcgaggg gattgcgcaa
    15601 ctgctttcgg cgcagattct tgccgggcaa tatgagcggc aaaaagcgat gctcacccag
    15661 tcagaaatca aactgcttca cgcccaggtg aatcctcatt ttttgtttaa tgcgcttaac
    15721 accattaaag cggtgatccg ccgcgacagc gaacaggcca gccagctggt gcagtatctt
    15781 tccacttttt tccgcaaaaa cttaaagcgg ccttcggagt ttgttactct cgccgacgaa
    15841 attgaacatg tgaacgctta tctgcaaatt gaaaaggcgc gcttccagtc gcggttgcag
    15901 gtcaacattg ctattccgca agaattatcc cagcagcaat tgcccgcgtt taccctgcaa
    15961 ccgatagtgg aaaacgccat taaacatggg acatcacaac tgctggatac agggcgagtg
    16021 gcaatcagcg cccgacgtga ggggcaacat ttgatgctgg agatcgaaga caatgccggt
    16081 ttgtatcaac cggtaaccaa tgccagtggg ctggggatga atctggtgga taagcgttta
    16141 cgtgaacggt ttggcgatga ctatgggata agcgtcgcct gtgagcctga tagttacacc
    16201 cgaataacgt tacgactacc atggagggac gaggcatgat taaagtctta attgtcgatg
    16261 atgaaccgtt agcacgggag aacctgcgcg tatttttgca ggagcagagc gatattgaaa
    16321 tcgttggtga gtgttcaaac gccgtggaag ggatcggcgc ggtgcataaa ctgcgcccgg
    16381 atgtgctgtt tctcgatatc cagatgccgc gcatcagtgg tctggaaatg gtggggatgc
    16441 tcgacccgga acatcgcccg tatattgttt ttctcactgc gtttgacgaa tacgctatta
    16501 aagcctttga agaacatgcc tttgattatc tgctgaagcc aattgatgaa gcgcgactgg
    16561 agaaaacgct ggcgcgattg cgtcaggagc gcagcaagca ggatgtttcg ctgttaccgg
    16621 aaaatcaaca ggcgctgaaa tttatccctt gtacggggca tagtcggatt tatttgctgc
    16681 aaatgaaaga tgtggcattt gtcagcagtc ggatgagcgg tgtctacgtt accagccacg
    16741 aagggaaaga gggctttacc gaattgacat tacgtaccct ggaaagtcgt acaccactac
    16801 tgcgctgcca tcgtcagtat ctggttaacc tcgcgcattt acaggagatt cgtctggaag
    16861 ataacggcca ggccgagttg attttgcgta atggcttaac cgtgccggtc agccgccgtt
    16921 atctgaaaag cttaaaagag gcgattggcc tgtaaaagac tgctaaaatg gctttttgcc
    16981 tcatcaacac ctgaaggcct catgctaagt aacgatattc tgcgcagcgt gcgctacatt
    17041 ttgaaagcca ataataatga cctggtgcgt attctggcgc tgggtaatgt cgaagccacc
    17101 gcggaacaga tcgccgtctg gctacgtaaa gaagacgaag agggttttca gcgttgtccg
    17161 gacattgttt tgtcgtcatt cctcaatggc ctgatttatg aaaaacgcgg caaggatgag
    17221 tctgctccgg cactggagcc ggaacgtcgc attaataaca acatcgtgct gaaaaaatta
    17281 cgcatcgcgt tttcgctgaa aaccgatgac attctggcga tcctcaccga acagcagttc
    17341 cgcgtttcga tgccggaaat tacagcgatg atgcgcgcac cggatcataa aaacttccgc
    17401 gaatgcggcg atcaattttt acgttatttt ctgcgtggac tggcagcgcg ccagcatgtg
    17461 aagaaaagct aagacgggta tggcggccat gcgaaacatg gccgccgaca gattatttca
    17521 cttctttaaa accagcggct ttcatcacca gttccatttg cgccatagtg ataccttttt
    17581 tggcatcttc agcagaaacg ttgattcctg aaataccctg cagggcttta aaatccactt
    17641 tttccatatc gatagtcacg ttttcctgcg cgtaggtatc ggtataggtt aatttttctt
    17701 caacacccgc gatgtttttg tatttggcgc ttaacggctc aagtgccctg gcagcatctt
    17761 ctttggtggt tgcaccaatg gaggcaaatt gaattttggt ttcagaagat tgcttaagca
    17821 ccttgtcacc tttgtagaca taggtaatgg caatttcagt gccgttcaga ttggcgctga
    17881 atttcttcga ttcttctttg tcaccgcagc cagcaagaga gaaaaccaga acagatgcaa
    17941 caacgaggga aaacagctta ttgaaagcct tcatgtaaaa ctccatttta tttaatcaag
    18001 aaactggtga ctctcaccag gggctatata gaatatgcct aataccgtgg cgtgagcagt
    18061 ccggaactgg agtagaactc ttagtaaaaa gcactatttc atccttgttg ctgaagcatg
    18121 gggaataatt gttcgcaaag caaaacaccg ttattcattg cttctacccg tgcctcgctt
    18181 tctgtattac gaaattgtgc caacacatgt gccagccgat aaaaacccac cgcggtgagg
    18241 tcattcgcca gcaactctgc ctgactaata gcgctctgtt cctgatagcg ccagccgtta
    18301 tggagcagtt gaataagtaa cgcctggcag cgcatcagca actgatgagc agtagacggc
    18361 acaggcagaa cgctggcaga aggtagtgat accaccacag gcgcagtttc tgcgtccagc
    18421 gcccaggcac gagtctttgt catcattacc tgtggttcca gtgtcaattg cccatcaaca
    18481 aaactgacaa agccagaaac cagatacacg gggtcgtctg tttgttgcaa aagcgccgcc
    18541 atgcgttcaa cggcataagg cgcgctggct gaggctggca gtgataacgt cagcagatta
    18601 tcttcacctt cgccgctgat tacctgcgca tccagcgtct ggcggctgct atcccaaccg
    18661 agcgaaatac actcagcgac cggcagaata aataagttat cgacctgatt aagaggccgt
    18721 atgcaggcgg ggggacgctg gcgtaaatat tcccgcaaag ccacaatgcc cggctggcgt
    18781 aacggcgcgc tcaacatttg ccaggcatca ggcgacagcg gcacaacgct gcttaagcgg
    18841 ttgcgggtag ctaacagcag ctcgccatcg gcactgcgtt ttgctgcttg tgaaacaatt
    18901 tgcccgcccg ccagtgcgcc agcctgaaaa ctaaacagcc gacgcgtagc tgccggtgag
    18961 ttttcctgtt cacttcgcgg ccaactgcgc gaaaggtgca aaatactgcc ggtgtcggga
    19021 tcggtaaacc agatgcgtaa accataatgc tcaatatcct gccagcaacg catacctaaa
    19081 gacaccagcc gcagatgatc aagctttgct tctccggcaa tgcctgcgcc aacgaccgtg
    19141 cgccacggca tgggaggaat ttcaccaaca ctgtcgcgct gcgccatttc gtgtgcgcaa
    19201 tttaatcgac tgtttaatgc ggcaagctga cacaagcatt ctccggcatg ataatggctt
    19261 gcgcgggcgt ggaaggcatc aacgctggcg cgcagttgcc gtagcgattc actcacccat
    19321 cgccagttgc agcgttccgc cgcttgctgc gcgcggctga aagcggcctc gtagtgaata
    19381 agcggctggc tgatgccgcc aagccataat gcctggctta attgctaaac atattgacga
    19441 cacgtttggc cttcttcgct ggcaaacgga tcgtcagatg atgtgacgtg ttcgctgcgc
    19501 atctgccaga ttaaatggtt aaattctgct tgctgcgctt tggcctcgac gaaggcctgt
    19561 accgccagta cgatatgttc gcaaagagtg ccttcaatac aatcacaacg ggcgaaacga
    19621 atgctgctgc gggaataaaa acgcacatcg ctcatcggta agcgggcaga gggaatttcg
    19681 cccggcgtac agaacaactc aatggtgatg cctttagcga ccagcgcctg cgcgcgtttg
    19741 cgggtggcat cgggcagggt agccagttct tccagccaga ttgccggatc ccactcttct
    19801 tctttttccg caggctgggc ggtagcacaa agtcgttgat aacttaacac cagcatcacg
    19861 cgatgacggc acatgccgct ggccccacag gtgcactgag cctctttcag tgcctggccg
    19921 ttcgccagct gggtacggac accgtcactg aaggtggcga ttaaagcgcc gttctcatgg
    19981 ctgatttccg ggacgttgcc attttccagt tccttaaggc tgcgcttaac aaaaccggca
    20041 ttgcttaacg ccgtcagtgc ctgcggtgtc agttctaata attccggacg tagtgaattc
    20101 atgactgaag attctccgca agccatgatg ccagctcgcc cggcgtcatg gcggctattt
    20161 gtgcgccgac attaaccagc gcctgggccg tatcgcggtc atagcaaggt gttgcggtgc
    20221 tatcgagcgc tgccagtccc agcactttga tgccgctctg gacacacttt ttcacctgat
    20281 gcgtcagtaa tgatgatgaa cccccttcgt aaaaatcgct cacgaggata atgacgcttt
    20341 tcgctggttg ttcaataagt tgccgaccat actccacggc actggcgata ttggtcccgc
    20401 cgcccaactg tactttcatt aataactcta ccggatcggc aacgtctgcc gtgagatcaa
    20461 cgacgcttgt gtcaaacgcc accagatggg tacgaatgcc gggtaactgc cacaaacagg
    20521 ccgccatcac cgcagagtgg atcaccgaat cgaccatcga tccgctttga tcaaccagta
    20581 agaccagttg ccattgttcg ctttggcgtt taatgcggct gttaaagcgg ggggattcga
    20641 tatacaactt gccgtgttgc gggtgccagt gttgcaggtt ggcgcgcaga gtacttttga
    20701 aatcaaagtt tcgcgccagt ggaataaatg aacggcgacg gcgatcgcgg acaccagaaa
    20761 aagcctgacg aacttccttt gccagtcgag ccataatttc ttcaacaacc tggcgcacta
    20821 tccggcgggc ggtagccagt acttcggggt tcatcagatg tttggtatgc aaaacggcgc
    20881 gtagcaggct ttcagaaggc tgcatacgtt ccagcacgtc gagattcgtc accacatctt
    20941 caatgccata gcgcagtacg gcatcgcttt ccagccgctc aatcacctgt tgcggaaaca
    21001 gcgtgtgaat actgttgatc cactcagggg tggtgagatt tgagccacct aatccaccgg
    21061 agcgttcacc acgctggagc cgttcaggat cgcgcccata cagccattcc agcgcgtggt
    21121 ctatctgccg ggcgttgtca tccagcccac aaagcgtcgt ttctgccgct tcgccaagaa
    21181 ttaatcgcca gcgttgtagc tcacgggtgg tcagaagatc gttcagttca gacatggaaa
    21241 actccccagt gttgcagcga tgccagtgcc tgctgttcga gttgttgatg atgtgcaatg
    21301 gcctgtggtg gacaatgcaa cggcatttgc agcgctgaaa caggaagctg cgcaagctgg
    21361 taatgctcaa gcacctgatg cgccagcgtc ccgcgttctc gtggcggtag ccaggccatt
    21421 gccgcgcgta aatcgggcag ggcgttaata aaatcgtcgt cacttagttg atttaaatga
    21481 ctgctgaaac cggcgataaa tgcaggttga caggccagtt gatgacgggc taatgccagc
    21541 aaaccgtgca gcgcctcacc ggattgtgcc ggggataact gcgccagcat cgtcagcgcc
    21601 gcttcggcac tggcgttggg atgttccaga cgcattagtg cgccaagagc cgcgccgcga
    21661 tccagcgccg gggcatgaat cgcctgactg cgtcgctcca gtaaggctac cgccgcagaa
    21721 agagaaacgc cgggtaaatt aacgccgcta tgtagatcgc gcaggatatg gcaaagcgct
    21781 tgccagctat gcaggtgagc gtgaaactcc ttttcatccg gtctgccgtt agattcacac
    21841 agccacagcg tgcgatcgat agccgcgcat aacgtcgttt gtaatatctg cgcgccttgc
    21901 ataccgctaa tttcatccag ccgccataag gcatatagca cttccagcgc ctggcccatt
    21961 tcggcaaatt gattttcctg ggcgattaat aatgtgagtt gctccagtaa ttgttgactg
    22021 aaggacgcca gacccgctaa cgccgcctgg ctaagacatg tggtgatact gccgattcca
    22081 cccgtgtcca gcatatcggc ttctaattta tggcgtgcgg cttcctggag tgttgcacca
    22141 aagcaggcgg cctcaatcaa tgccgcatgc tggctaagcg ggcgggttaa tttccagtgt
    22201 tcttcaccgt tgcctgcaag tgtcagtgta cttccctgct ggcgtacaat ccctgggatc
    22261 tccagtattg ccagccgatg taacacctga ctttgcgcta gtccattggg gttaaagcga
    22321 tttaaggtaa gctccgccgg aagagagatt gcggtacgtt ccagttcgca ggtgacatct
    22381 tttggcagag gcggttgtgg cgtagaaggg gcaagtttac cgaatccgtc acccgctaat
    22441 gtgtctatca acgttagcag aatcggatcg gtatcgggat gaataacgcc gcgatagctc
    22501 cacggcaacg gcgcattcag ggcttcttta atcagcgagc ctgctaaggc atccagccag
    22561 tcagtgcgta acggtagtgt atgaccgcgc aactgtgcca gcgccatcgc atgcagatga
    22621 gcggcagcca tatccgcggt agaagcgggc aatttgtgct ggcgcaaacg ggtaaggata
    22681 gtttttagca gttgttcacc ggccttctgc aagccccact gccagcacca gttttgccat
    22741 accggggcag gcattcctga aaggtatcct gccagcacat caaggcgctt ttcactgtag
    22801 ggtgtgagat aacaatctgt aacggcatct gccagcgagg gcaattctgg cttgttaatt
    22861 tcctgagggc attcgcgcca catctttgcc agtgccggag cgtgccagcc accgcagaca
    22921 accaatacgt cgccatcatt ttgctgcatc gcccatccaa tccagcgggt cataaaggct
    22981 tcgcgctgac gattgagtac atcgccgggg gaatcgccgc gcagttgggc aaaatagtgc
    23041 gccagcgcag agggtaatgc aatttgctgg ctttcatctt cgaacaagtg atcccacagg
    23101 gtgtcgctgt tatccatgcg ggtggcacgc agcagcaagg tctggctttc ctcttgcgta
    23161 tcaggcgaat cgtcctcttc ttcactctgc gcccagcaag gcaaatcgat gaagtaagtt
    23221 tgtgcctgaa tacgacgagc ggcttgtagc gcctgccact ccggcgaaaa ttcagcaaat
    23281 ggcgtccagg caccacgccc gggggctgca ccgtcctgat actggcaata actgtaaata
    23341 gctaccggaa gctggtgggc taaaaacagt tcgtctaccc ggtcattaaa atcagccggg
    23401 ccttcaatca acacgtatcg tggccgctgg ctttcgatta acgatttcac cagccgggcg
    23461 caggccggac tatgatgccg gatgccgaca attaacggct cgctcatgca tctttcctca
    23521 cggcaggcgg tggcgggctt gataataagc ctgccagtga gcttctttat gtgtggcgac
    23581 acgctgttcg aagtaacggc gcagacgagc gcgatcttct tcattatctt tgacgatggt
    23641 tccggcaatg cagtccacca ggtcggctgg ctcacctgcg cgattcgcta aaaaccaggc
    23701 gcggacgcct acggcgtggg ccacgttcac ggcttcagcg gtggacataa tcgctgttag
    23761 tgtatccatt gacgttttct tttcgccgtt ggcacgcaga tcgcggaagg tgcggaccag
    23821 taactccagt accgcgtcag gcactttatg cggaataccg ctatgggcca gcaaacgcgc
    23881 cgaagcactg gcgacaagct ccagctcctg ggcgaaatcc ataatcggaa agacagtttc
    23941 aaaatcgaag cgacgcttta gcgcagcact catttcattg acgccgcgat cgcgggtatt
    24001 ggcagtggca ataatattaa agccttctcg cgcatagagc tgactggctt caccagtgag
    24061 ttccggcacc gtcatcaccc tgtcagaaag catacctaac agacagtctt gcacttccag
    24121 cggtgtacgc gttatctctt caaaacggac gattttgccg tcgcgcattc cctggtaaag
    24181 cggggcaggg accagcgctt ccgttgatgg accatgattg ataagcaggg cgtagttcca
    24241 gccgtacttg atttgatctt cggtagtaga tgcgccgccc tggatggtta accaggcatc
    24301 gccgctaata gcggttgcca gtaattcaga caggagagat tttgcggtgc caggttcccc
    24361 caccaacatc aaaccgcgtc cggtggcaag agtgactaac atacgttcga cagcggaggg
    24421 actaatcacc actttacggc taatattttg cgcgctatcg ccaaggataa aagcacgtgc
    24481 cgcaggcaaa cttaactgcc aaccgggcgg gcaaggtgcg ttatcatttt gttttaattt
    24541 tgccagttca tcggcgtata acacagcagc aggcggacgc tgcagatgat tattctgcgg
    24601 tgacatatat tcatccctga aatgtattaa tgaaataaac gatattttac gcgcaataaa
    24661 tgtttggcgc attagttgtt tgagcggaaa atatctggaa agcagatcac agtaaataaa
    24721 aaaacctgct ggtgaggcag gttcttttat ttactatttt caggcccaac accgcagcct
    24781 gtgcaacatc gtgttccaac gaacgaatta taaccacggc tggaccccgt tgcaattaag
    24841 ataatgagat ttataatcca ggccattggg acgttgcgcc ggatcggacc aatttgaatt
    24901 atttagagta tatt
//
LOCUS       DAPHBJ010000054        24346 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.054, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000054 DAPHBJ010000000
VERSION     DAPHBJ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.054"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            18..647
                     /locus_tag="RHZ76_001928"
     CDS             18..647
                     /locus_tag="RHZ76_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HDV2812578.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALLYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            674..2335
                     /locus_tag="RHZ76_001929"
     CDS             674..2335
                     /locus_tag="RHZ76_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDV2812579.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGAAGDASTGSVSGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLTALTSPLTSTTPVAENVE"
     gene            2574..2633
                     /locus_tag="RHZ76_001930"
     CDS             2574..2633
                     /locus_tag="RHZ76_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDV2812580.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            2949..3008
                     /gene="ibsE"
                     /locus_tag="RHZ76_001931"
     CDS             2949..3008
                     /gene="ibsE"
                     /locus_tag="RHZ76_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HDV2812581.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3128..4561)
                     /gene="hldE"
                     /locus_tag="RHZ76_001932"
     CDS             complement(3128..4561)
                     /gene="hldE"
                     /locus_tag="RHZ76_001932"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HDV2812582.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(4609..7449)
                     /gene="glnE"
                     /locus_tag="RHZ76_001933"
     CDS             complement(4609..7449)
                     /gene="glnE"
                     /locus_tag="RHZ76_001933"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HDV2812583.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYAAWLQEALCNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAELHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGSWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(7472..8773)
                     /gene="ygiF"
                     /locus_tag="RHZ76_001934"
     CDS             complement(7472..8773)
                     /gene="ygiF"
                     /locus_tag="RHZ76_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HDV2812584.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9015..9635
                     /locus_tag="RHZ76_001935"
     CDS             9015..9635
                     /locus_tag="RHZ76_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDV2812585.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            9699..10937
                     /gene="cca"
                     /locus_tag="RHZ76_001936"
     CDS             9699..10937
                     /gene="cca"
                     /locus_tag="RHZ76_001936"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HDV2812586.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLALM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(11118..11939)
                     /gene="bacA"
                     /locus_tag="RHZ76_001937"
     CDS             complement(11118..11939)
                     /gene="bacA"
                     /locus_tag="RHZ76_001937"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDV2812587.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTSGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12029..12397)
                     /gene="folB"
                     /locus_tag="RHZ76_001938"
     CDS             complement(12029..12397)
                     /gene="folB"
                     /locus_tag="RHZ76_001938"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HDV2812588.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            12502..13119
                     /gene="plsY"
                     /locus_tag="RHZ76_001939"
     CDS             12502..13119
                     /gene="plsY"
                     /locus_tag="RHZ76_001939"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDV2812589.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13132..14064)
                     /gene="ttdR"
                     /locus_tag="RHZ76_001940"
                     /gene_synonym="dan"
     CDS             complement(13132..14064)
                     /gene="ttdR"
                     /locus_tag="RHZ76_001940"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HDV2812590.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            14271..15182
                     /gene="ttdA"
                     /locus_tag="RHZ76_001941"
     CDS             14271..15182
                     /gene="ttdA"
                     /locus_tag="RHZ76_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HDV2812591.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLSFENLSHTRSAL"
     gene            15179..15784
                     /gene="ttdB"
                     /locus_tag="RHZ76_001942"
     CDS             15179..15784
                     /gene="ttdB"
                     /locus_tag="RHZ76_001942"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HDV2812592.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            15833..17296
                     /gene="ttdT"
                     /locus_tag="RHZ76_001943"
     CDS             15833..17296
                     /gene="ttdT"
                     /locus_tag="RHZ76_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HDV2812593.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWMAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIICWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17339..18352)
                     /gene="tsaD"
                     /locus_tag="RHZ76_001944"
     CDS             complement(17339..18352)
                     /gene="tsaD"
                     /locus_tag="RHZ76_001944"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDV2812594.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            18589..18804
                     /gene="rpsU"
                     /locus_tag="RHZ76_001945"
     CDS             18589..18804
                     /gene="rpsU"
                     /locus_tag="RHZ76_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDV2812595.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            18915..20660
                     /gene="dnaG"
                     /locus_tag="RHZ76_001946"
     CDS             18915..20660
                     /gene="dnaG"
                     /locus_tag="RHZ76_001946"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDV2812596.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDDEDPDTLIRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            20855..22696
                     /gene="rpoD"
                     /locus_tag="RHZ76_001947"
     CDS             20855..22696
                     /gene="rpoD"
                     /locus_tag="RHZ76_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDV2812597.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHATAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(22775..23281)
                     /gene="mug"
                     /locus_tag="RHZ76_001948"
     CDS             complement(22775..23281)
                     /gene="mug"
                     /locus_tag="RHZ76_001948"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HDV2812598.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQASEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            23406..23481
                     /locus_tag="RHZ76_001949"
     tRNA            23406..23481
                     /locus_tag="RHZ76_001949"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23439..23441,aa:Ile,seq:cat)
     gene            complement(23534..24298)
                     /gene="nfeF"
                     /locus_tag="RHZ76_001950"
     CDS             complement(23534..24298)
                     /gene="nfeF"
                     /locus_tag="RHZ76_001950"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HDV2812599.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHEDGVASSWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSVLVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFETEQFDPQRVRAAAYWHAK"
BASE COUNT         5874 a         6667 c         6209 g         5596 t
ORIGIN      
        1 ggacattgga gtgatatatg attttattcg ccgactataa caccccttat cttttcgcta
       61 tttcctttgt actgttgatc ggcctgttag aaatttttgc gcttatctgc ggtcacatgc
      121 tctccggtgc tcttgatgca catcttgacc attatgattc cataaccaca ggtcatatta
      181 gccaggcact tctttatctc aatattggaa gattgccagc tctcgtcgtt ctctgcttac
      241 tggctggttt ttttggtctt attggcatcc tgttgcagca tgcctgcatc atggtctggc
      301 aatcaccact gtcgaacctg ttcgtggttc ctgtcagttt actgtttacg attattgcgg
      361 tgcattatac cggtaaaata gttgcgccct ggattccacg cgaccatagt tcagctatta
      421 cagaagaaga atatattggt agcatggcgt taattaccgg tcatcaagcc acttcaggta
      481 atccctgcga aggaaaactc accgatcaat ttggccaaat acattattta ctactggagc
      541 ctgaagaagg aaaaattttc actaaaggag acaaagtgtt aattatttgc cgactttctg
      601 caacgcgata tttggcggaa aacaatccct ggccccaaat attgtaattt attaatcaaa
      661 aggaaattta ataatggatg atattgttaa ttctgtgccc tcctggatgt ttaccgcgat
      721 tattgccgta tgcattctgt ttattattgg aattattttc gccaggctct atcgtcgcgc
      781 ttcggcagag caagcttttg ttcgtactgg tttaggtggg caaaaagtgg taatgagcgg
      841 tggcgcaatc gtgatgccga tctttcatga aataatcccc atcaatatga atactctgaa
      901 gctggaagtc agtcgctcaa ccattgatag cctgattacg aaagatcgta tgcgcgtcga
      961 tgtagtagtc gctttctttg tgcgggtaaa accttcagta gaagggattg ccaccgctgc
     1021 ccagacgctg gggcaacgca ccctgtcgcc tgaagactta cgtatgttgg ttgaagataa
     1081 atttgtcgat gccctccgtg caacagctgc gcaaatgacc atgcatgagt tacaggatac
     1141 ccgcgagaac ttcgtgcagg gggtgcaaaa tacagtggca gaagacctgt cgaaaaacgg
     1201 tctggaactg gagagcgttt cacttaccaa ctttaaccag acctcgaaag aacatttcaa
     1261 tccgaacaat gcctttgacg ccgaaggttt aaccaaactg actcaggaaa cagagcgccg
     1321 tcgccgcgaa cgtaacgaag ttgaacagga tgtagaagtt gcggtgcgtg aaaaaaatcg
     1381 cgatgcgcta tcgcgcaagc tggagattga acagcaagaa gcgtttatga cgcttgagca
     1441 ggagcagcag gttaaaaccc gtactgccga acagaatgca cgtattgcgg cttttgaagc
     1501 tgaacgtcgt cgtgaagcag agcagacacg aattctggct gaacgacaga ttcaggaaac
     1561 agaaatcgac cgcgaacagg ccgtccgctc aagaaaggtt gaagctgaac gtgaagttcg
     1621 cattaaagag atcgaacagc agcaggtcac cgaaatcgct aaccagacga aatcgatcgc
     1681 tattgccgcc aaatcggaac aacagtccca ggcagaagcg cgtgctaatc tcgcacttgc
     1741 agaagcggta agcgcccaac aaaacgtaga aaccactcgc cagactgccg aagccgatcg
     1801 tgctaaacaa gttgccctaa tcgctgccgc gcaggatgca gaaaccaaag cggttgaact
     1861 gaccgtgcgg gcgaaagcag aaaaagaagc cgcagagatg caggcggcgg ctatcgttga
     1921 gttagccgaa gctacacgta aaaagggtct ggcggaagca gaagcacaac gtgcgctgaa
     1981 cgatgctatc aacgtacttt ctgatgaaca aaccagcctt aaattcaaac tggccttgtt
     2041 gcaggcgctg cctgcggtaa tagagaaatc cgttgagccg atgaagtcaa tcgacggtat
     2101 caagattatt caggtcgatg gtctgaatcg tggcggcgct gcgggtgatg cgagtacagg
     2161 tagcgttagt ggaggaaacc ttgcagagca ggcattgtct gccgcccttt cttaccgcac
     2221 acaggcaccg ctgattgact ccttgctcaa tgaaattggt gtttcaggcg gctcactgac
     2281 ggcattgact tcacccttaa cctcaacaac tcccgtcgcc gaaaacgtag aataaatttt
     2341 cagctatgaa cgtccctggc acaaaaagcc ggggattttt tatatctgcg ttccgctaaa
     2401 aagtgcaaat gatcaggttg ttgcagcatt ttgcgtgacg gctcggggaa ggcaaaactg
     2461 catcagagaa agcattgcgt ggggtctggc gctcatcaac aatcggggag tcagcagggg
     2521 gctgaaacgg gaaagcccct cccgaagaag gggccttaca gaaggaaagg gttatgatga
     2581 agctcgtcat catactgatt gtgttgttac tcgtaagttt cgcagcttat taacagccaa
     2641 tcagaggggg gagaaatcct ccctcaccct tgttccttta ctttaggttg aaaaaaaatc
     2701 agcgtcaaga aggtttcatt aacacatatc cctggcccaa aaagccgggg attttttata
     2761 tctgcgttcc gctaaaaggt gcaaatgctc aggacgttgc agcgttttgc gtgaccgctc
     2821 ggggaaggca aaattgcctc tgggaaagca ttgcgcgggg tccggcgctc atcaacaatc
     2881 ggggggcagc aaggggctga aacgggaaag cccctcccga agaaggggcc ttgtataagg
     2941 aaagggttat gatgaagttc gtcatcatac tggttgtgtt gttactgtta agtttcccga
     3001 cttactaaca actcatcaga ggggggagaa atcctccctt acccttgttc ctttactcta
     3061 ggttgaaaaa acaacagcgt caataggcct gccatgtacg aagcgagatc tgtgaaccgc
     3121 tttccggtta gcctttttta tcctgttgga tcttcttgat gatgttggtc gtcgagcaac
     3181 cgtcttcaaa gttgagcacc aacacttcgc caccgttggc ccagacttct ttactcccgg
     3241 caatctcttc tggtttatag tcgccgcctt tcaccagcag atctggcaag atcccggcga
     3301 tcaagcgctg cggcgtgtcc tcttcaaacg acaccaccca gtcgaccgct tccagtgcgc
     3361 ccagcacaat catacgctgt tcgagcgggt taacggggcg ggaatcccct ttcagccgtt
     3421 tggtggaggc atcgctgttc acagcgacaa tcaaacggtc acccagttta cgggcatttg
     3481 ccagataaga gacgtgcccg gcgtgcagga tgtcaaagac accgttggtc atcaccactt
     3541 tttcaccacg tttacgcgct gccgctacgg ccagcttcag ttcctcttcg gtcatcacgc
     3601 caaagcctgt atctgcacgt ccacgtacag cattttccag ctcgatcggc gaaacagtgg
     3661 aggttcccag tttgccgacc accacgccag ccgccgcatt ggcaaagaag caggcttctt
     3721 ccagcgaatt acccgctgcc agcgttgccg ccaggacgcc aatcaccgtg tcgcccgcac
     3781 cggtaacgtc atacacttcc tgcgcctggg ttggcatatg cagcggcgct ttacccggtt
     3841 gcagcagcga cataccctgt tcggaacggg tcactaacag agccgagagt tcgtaatcgg
     3901 caatcagttt catgccgcgc tcaacaatct cttcttcggt cttacattta ccgacaacag
     3961 cttcaaattc cgagagattc ggcgttaaca gcgtagcgcc gcggtagcgc tcaaaatcgg
     4021 taccttttgg atcaatcagc accggaacac ccgctttacg cgccagttgg atcatctgct
     4081 gtacgcttgc cagcgcacct ttggcgtagt cagaaagcac cagcgcgcca atcgaactca
     4141 gcgcctgatt aatccgctcg tgcagcggct gcggatcaac accttcgaaa ccttcttcaa
     4201 aatccagacg gatcagctgt tggttgcggg aaagtacccg taatttggta atggtcggat
     4261 gcgtcggtac agaaacgaag tcacatttga cgttgacgtc ggccagagat ttactcagcg
     4321 cgcgcgctgc atcgtcaatg cccgtcaacc cgaccaggcg tgcattagca ccgagagaag
     4381 cgatattcat cgccacgtta gccgcgccgc ccggacgttc ttcgatggta ttcactttaa
     4441 ccacgggcac cggcgcttcc ggcgagatac gactggtggg gccgtaccag taacgatcca
     4501 gcatcacatc accaaccacc atcactcctg cacgttcaaa ctctggcagc gttactttca
     4561 ttcctgtctc ctgagagatt caaaatttgc gcgcgataat accatacttc attcttccac
     4621 cagccacttc tgccagcttg cccgcaccag ttcacgctct gcggtgaagc aatcctccga
     4681 cacatggccc ggcaattcct gtaatgccag atgatgaagt tcatcgcgaa gcgtagtgta
     4741 agcacgggtc agcgccatcg cttcctgctc ttccataatg tcgttttgcg ccagtagttc
     4801 cagaatacgc acgttgtctg accagcgcgt taacttcggt ttttcatgag cgtagcgcaa
     4861 caccagatat tgggtaataa attcgatatc ggtaattccc ccttcatcag ctttgatatc
     4921 aaagcgatcg cgatgtttat tgccgagatg agcgcgcatt ttctcgcgca tttcccgcac
     4981 ttccgtttgc agagttttac cttcacgcgg cagcgtcata atctcgcgac gcactgcgtc
     5041 aaagtgcgcg gtgagctgcg gatcgccgta cactacacgc gcacgcacca gcgcctgatg
     5101 ttcccacgtc caggcctcgt ttttctgata atcggcaaat gcttctgcgg atgtcaccag
     5161 cattcccgcc gccccggacg gacgcagtcg agcatccact tcatacaaaa tgccggaaga
     5221 ggtacgcgta ctgaacagat gcataatgcg ttgcgccaga cgcagataaa actgccgccc
     5281 gtcgatttcc cgctcaccgt cagtcatcgc atccattggg caatcatgga ggaagataag
     5341 gtcaagatcg gaactgtagc ctaactccca gctgcccagc ttgccgtagc cgaccaccgc
     5401 aaaaccacgc ccttcgcgtt cgttcaggtg attcggctta ccgtagcggg caaccatttg
     5461 aacccacgcc tgctgaacga cggcatctat catggcttcc gccagccagg ttaagtgatc
     5521 gctcactttc atcaccggta gcgtaccggc gatatccgct gcggcgatgc gtaacagctg
     5581 cgcctgtttg aactgacgca gcgcctcaag ctgttgctct tcgtcatctt ccggcacgcg
     5641 cagcaaatac tggcgcaact catcgcggta ggcatcggtc gccgtcggct ggtaaagggt
     5701 gtttggatcg agcaattcat ccagcaataa tggataacgc gccagctggc tggcaatcat
     5761 cggcgacgcg gcacacagag aaatcaaatg tttaagcgcc gcggggaatt cactgagcaa
     5821 ttctaaatag gtggtgcggg taacaatccc caccagcaag gcggtaatgc gcgacagcgt
     5881 aacggcagcg tcttcacgcg cacagacatc acttagcaga tgcggcatca gatggtcgag
     5941 cacctgacgt cctcgcggcc cgatggtgcg cttatccagc tctttgcgga aatcggcaat
     6001 cagcgttagc acctgtttgc gatcatcctc gctaagatgc gccagcactg gcgtagtgtc
     6061 atcttcctgc aacgcatcct gccacagctc acgccactgt tccgacagcg actcttcctg
     6121 agtttcactt tcgtcgtcgc caatcaattc attaaacacc cggcgcacat tggtcatatg
     6181 tgcggtcagc gccccggtca attgcggcca gtcagcaaag tccatcgccc acgccagccg
     6241 cgcacgatta agctcatcag aaggaagcgt ctgggtttgt tcgtcgttaa tgctttgcag
     6301 caggttttcc agacgccgca ggaacagata cgccactcgc aattgttcag catcgttttc
     6361 agaaagcaga tgcagctcgg caatggcgct gagcgttggc agtaaagagc gcgattgcag
     6421 cgacggttcg cgtccgccgc gaatgagctg gaacacctga acgataaatt caatttcgcg
     6481 aatgccgcct gcgccgagtt tgatattgtc ggtcaaacca cgtcgacgca cttcacgggc
     6541 aatcatccct ttcatgttgc gcagcgactg aatcacgctg aaatcgatgt aacgacggaa
     6601 aacaaacggg cgcagcatcg cacgcaactc gttagcatag acgccttcgc tatcgcccat
     6661 aatccgcgcc ttgaccatcg cgtaacgctc ccagtcgcgc ccctgctcct ggtaataatc
     6721 ttccaacgcg gcaaagctca gcaccagcgg gccactttcg ccaaacggac gcagccgcat
     6781 atccacgcga tagacgaagc catcctgcgt tggttgatcc agcactttaa tcagccgctg
     6841 ccccatgcgg gtaaaaaact gcgcgttatc cagttcccgg cgtccaccct gcgtacaacc
     6901 atgttccggc caggcaaaaa tcagatcgat atcagaggag aaattcagct ccccaccgcc
     6961 cagcttaccc atgcctaaaa tcagcagcgg ttgcgcttcg ccctgcgcat tgcacggcgt
     7021 tccccactcg cggcagcagg cgtcatacag ccagtcacgc gccgcaacaa tcagcgtctc
     7081 cgccagatag ctgagctgct gcaatatgct ctcttcagta accagtgcca gcgtttgcgc
     7141 ccaggcgatg cgcaccataa tgcgccgccg gaatagccgc agctcgcgca ttaacccggc
     7201 ttcgtcactc acattacaga gcgcctcctg caaccatgcc gcgtaatgct gccattcgtc
     7261 ggcctgcggc ggttggcttt ccagttccgt cagccactct ggatgcgcaa tcacgctgtc
     7321 ctgcacaaaa tcactaaaag taagtactga cttcgcctgt gcgctaagtg attcctcggc
     7381 taaaggctct ggcagccgct caacaacggt ctgccagtac tgctgtaacg gtgaagagag
     7441 cggcttcata agcgatttta tccttgcctg attaacgttt tccgctgtgc aaccagaacg
     7501 gttcctgatt gtttgcctca ttacggaaat gttcaatttc gatgcgttgc ccggtcgcaa
     7561 tagcgtgatg cagcccctgc cagttctcca gccaggcttg cgcgacgaca ggatcatagt
     7621 aacccgccag caacagtatt gagtcaatat cacgcgtcag gcgtggcaac tggtcacggt
     7681 agcgatcgcc taacggctgg cagaaaacgc ttttcagttc agcggcatgg cgggaaagat
     7741 ggatatcggc aaagcgtttg aaggagtcgc tgattttgcc ctgcgctttg gcatctaaaa
     7801 atggctgcca tgctttgctt accaaccatt cggtcaacgc cagcttcgcc attgccgttt
     7861 cggtagagta gaccgccgtc acggcagaca ccgcagaagc aatggtcgcc tcgcattgag
     7921 tcagcagatc acgtaagtga gtgctcgctt tacgcggcac aataccaccg aacagcatca
     7981 gcgtatgacg gaccaggcta atggctgcca gcacctgttc tttcgccgca tcgttgccgc
     8041 gtacccacag ttcttcatga tactgccatt gcgctaacgc cagctcgagc gccgcttcca
     8101 gcccctgttc cacatcggct tttgccgcaa catgcaaaat ggtggtcggt ttgatttcac
     8161 gcgccggatt gccctgcgcc agatgatagc cacgcgccgc tttgctcagg ctgccctggc
     8221 gtaatccggt ttgcgatacc agttggttcg ccagtttcag caccgcgcgc gtgtcgccgc
     8281 taagcagttc cagttccagc tcacagatag gttcagcaaa ttcacccgct ttcacttccc
     8341 cctggtcgag ggcgatttca atttggctac catcgaccgc caccagccat ttttcgcgat
     8401 aaaaatcggt gctgaacagc ggctgcacgc gggaggcgag atcggcgggc aattcgccgt
     8461 tcggccagac ttccgtcggt aactgcgcca ggtcgagcgt cggttcgctc aacgccacgt
     8521 tatattccgg gcgctgatgt aagccgcctg tcactcttcc tgcaactttc atggtcatct
     8581 catagcgacc gttttcgcca cgaatacgta agcccatatc gtgcccacgc agccagttat
     8641 ccggcgtttc gtagtaaata ttcagcaact gcacggggtc atggtgctcg ccgcccagcg
     8701 tattgagatg gtcacgcaac gcctcaacgg cactgtgatt aacaataaac tttaattcga
     8761 tttcctgagc catggccttt acttatcggt tatgtcacat aagtgacgat gaacggcgaa
     8821 cttaatgcga tcttttttgt cagtagatag tattttgcgc caaattgcca tgcaacgggc
     8881 aatttgacgg gcgtaaaagt ttgaagcagt ggcaagacct gacacagagg attccgattg
     8941 atgtcgaatg ctttgcgtag cgacactgat gccactacta tcgttccact ttccatgaca
     9001 ataacgacag cctgatgcca aaattacgcc tgatcggatt aactttactc gcacttagcg
     9061 cgactgccgt ctcacacgct gaagaaacgc gttatgtttc cgacgaactg aatacctggg
     9121 tccgcagcgg tccgggagat cattatcgcc tcgtgggcac ggttaacgcc ggcgaggaag
     9181 tgaccttatt acaaactgac gccaacacca attatgccca ggtgaaagac agctctggcc
     9241 gtaccgcctg gatcccgttg aaacaactta gcactgagcc aagcctgcgc tcccgtgtgc
     9301 cagatctgga aaatcaggtc aaaaccctga ccgataaact caccaatatc gataacacct
     9361 ggaatcagcg caccgcagaa atgcagcaaa aagtggcgca gagcgacagc gtgatcaacg
     9421 ggttaaaaga agaaaatcag aaactgaaaa acgagctgat tgtcgcgcag aaaaaggtcg
     9481 atgccgccag cgtacagctg gatgacaaac agcgcaccat catcatgcaa tggtttatgt
     9541 atggcggtgg cgtgctgggg cttggcttgc tgctcggtct ggtactgccg cacctgatcc
     9601 caagccgcaa acgcaaagat cgctggatga actaaatcgc cttcttcgtt gcactgtctt
     9661 attattagga attgtgtatt tgacgaaggg atgatggcgt gaagatttat ctggtcggtg
     9721 gtgctgttcg ggatgcattg ttagggctac cggtcaaaga cagagattgg gtggtggtcg
     9781 gcagtacgcc acaggagatg ctcgacgcgg gctaccagca ggtaggccgc gattttcctg
     9841 tgtttctgca tccgcaaacg catgaagagt atgcgctggc acgtaccgaa cggaaatccg
     9901 gttccggtta caccggtttt acttgctatg ccgcaccgga tgtcacgctg gaagatgatc
     9961 ttaagcgtcg cgatctgacc attaatgcgc tggcccagga cgataacggc gagattatcg
    10021 acccgtacaa cggtctgggc gatctgcaaa atcgtctgtt gcgccatgtt tcccccgctt
    10081 ttggcgaaga tccgttacgc gtattgcgcg tggcgcgttt tgctgcgcgt tatgcccacc
    10141 tcggttttcg tattgccgat gaaactctgg cgttgatgcg cgagatgacc catgcgggtg
    10201 aactggaaca cctgacgcct gaacgggtat ggaaagagac ggaaagcgcc ctcaccaccc
    10261 gcaatccaca ggtgttcttc caggtactgc gcgattgcgg cgcactgcgc gttttattcc
    10321 cggaaattga cgcactgttt ggcgttccgg cccctgccaa gtggcatccg gaaatcgata
    10381 cgggtattca taccttaatg acgctctcaa tggcggcgat gctgagtccg caggtcgatg
    10441 tccgtttcgc aactttatgc cacgatctcg gtaaagggct gacgccgcca gaactctggc
    10501 cgcgtcatca tggtcatggc ccggcgggtg ttaagttagt ggaacaacta tgccagcgtc
    10561 tgcgcgtgcc aaatgaaatt cgcgatttag ccagactggt ggctgagttt cacgatctca
    10621 tccacacctt cccaatgctg aacccaaaaa ccatcgtcaa attatttgat tccatcgatg
    10681 cctggcgtaa accgcagcgt gtcgagcaac tggcgctgac cagcgaggct gacgtgcgcg
    10741 gcagaaccgg ttttgaatca gcggactacc cgcaaggccg ctggttgcgc gaagcctggg
    10801 aagtggcgca gtcagtgccg acaaaagccg tcgttgaagc gggatttaaa ggtgtggaga
    10861 ttcgcgagga gctgacccga cggcggattg cggcggtagc cagctggaag gaacaacgtt
    10921 gcccaaagcc tgaatgagta ttgggcattt tgccggatgc ggcgtaaacg ccttatccgg
    10981 ctacatgtca acgacagttg taggcctgat aagacgcgca agcgtcgcat caggcgttga
    11041 ttgccggatg cggcgtgaac gccttattcg gcctacgcaa tcgttgacaa cgccaagcat
    11101 ccgacactat tcctcaatta aaagaacacg acatacaccg cagccgccac aataaagcga
    11161 taaatggcga acgggataaa cgaaatgcgc ttaatcaatt gcaggaaggt tttaatcgct
    11221 atcagcgcca ccacaaaagc ggtgataaac ccaacggcaa acatcgggat atcgccgctt
    11281 gtcaggaagc cccagctttt gtagagatcg agcgccgttg cgcccatcat catcggcacc
    11341 gccagcagga acgaaaactc ggaagcagcg taacggctca cccccatcag catcccacct
    11401 gaaatggtcg ccccggaacg ggagaaaccc ggccacagcg ccagacactg gaaacagcca
    11461 atcataaatg cctgacgata ggtcatatca tcaagacccg gcgcacgcgg ctctttcggc
    11521 ttcaggcatt cggcggcaat cagcaacaaa ccgccaacga ccagcgcata catcacattt
    11581 atcgggttaa acaatgactt aatcgtgtcg tggaacaaca gccccaatac caccgccgga
    11641 atcatcccca gcaaaatgtg gatcagcgtt aaacgacctt tgctttcacc ttcgtgctgc
    11701 aacgggcggc caaagtggat gccaatcagg ccaaacagac gccgccagaa catcactact
    11761 accgccagaa ttgatcctaa ctggatcaca acttcaaagg ttttcgccgt gtcgccctca
    11821 aaccccaaca agtgaccgac aataatcata tggcccgtgc tggataccgg cagaaattct
    11881 gtcaatcctt cgaccacacc caatattgcc gctatcagca gcgagtgcat atcgctcatc
    11941 aataaacccc taaattatta aaatgtaccg cttgtccgaa ctactgcgta tgaccaggtt
    12001 ataaccgttt ggtttaacag ctgtaaaatt aattattttc tttcagatta ttgccacgct
    12061 caatgattac gccaacattc gccgcccgcg ccactgcgcc tggcttgctg agtttgatac
    12121 gcacccacgg cgagttgaag cgtgctaaca gcagctccgc cacctcttca gccacgcgtt
    12181 ccaccagcgc aaaacgcgcc ccctcgacgt ggctgaccac cgtttctgca atgtcagcgt
    12241 aactgaggca atccgccaca tcatcacttt tcgccgcttt acggttatcc cacgccattt
    12301 cgatatcgaa cactaacttc tgttcgatgg tctgttccca gtcgtaaaca ccaatagtgg
    12361 tgattaccga aagttgctct ataaatacaa tatccatcac gtcctgcctg ctttttggct
    12421 aaccggatac cacttccggc gaaatgtgcg tattatccac agattcatcg ttgaacacga
    12481 attttcaaaa cggaacagct tatgagtgca atcgcgcctg gaatgatcct catcgcgtac
    12541 ctctgcggct ccatttccag tgccattctg gtttgccgct tgtgtgggct gcccgatccg
    12601 cgaaccagcg gctccggcaa tccaggcgca accaatgtgt tacgtatcgg tggcaaggga
    12661 gcagccgtag cagtactgat tttcgacgtt ctgaaaggaa tgttgcccgt ctggggcgcg
    12721 tatgaattag gtgtcagccc cttctggcta ggcttaattg ccatcgccgc ctgtcttgga
    12781 cacatctggc ccgttttctt cggatttaaa ggaggaaaag gcgttgctac cgcttttggt
    12841 gccatcgcac ccattggctg ggatctcacc ggagtaatgg cgggaacctg gttactgacc
    12901 gtgctattga gcggatactc gtcgctggga gcgattgtca gtgcactgat tgctccgttt
    12961 tatgtctggt ggtttaagcc acaattcacc ttcccggttt cgatgctctc ttgcctgatc
    13021 ctgctgcgtc atcatgacaa catccaacgt ctgtggcgtc gtcaggagac aaaaatctgg
    13081 acgaaattca aaagaaagcg cgaaaaggat cccgagtgat ttctggtgga tctacatgac
    13141 ctgatagcct tcatcgggct tgcccagccg ttgctggcac catgccgcca gaaattccac
    13201 gcagacacgt aatttcatgc tgcgatagag cggctcccgg taaacagccc agatattggc
    13261 gctttgtgca tactctggca atacttgcac cagtttgcca ctctccagaa acggcaacac
    13321 atcccactcg gaacgcagca taatcccttt gccctccagc gcccattgca gcacaatctc
    13381 gccgctattg gaggaaagat gcccgcttac cttcaccgat tttttctcct gaccgttccc
    13441 caactcccat attccatggg tcatatcgcg ctctttggtc accagacagt catgacgact
    13501 taattcctgt agggattgtg gctgcggata tttttgcaga tactcaggtg ctgcgcataa
    13561 tattctttta ttttttgtca aaagatgcgc aatataataa tcaggaattt cgtcattaat
    13621 acgaatatcc agatcaatat tatcctgcac taaatcaatt tgccgatcga acaattcaaa
    13681 atgcacctgt aactcaggat aattgcgcat cagttcggta atagctggcg caatatggct
    13741 gcgcccaaaa ccaaaactgc aaccaatacg aatcatcccc tccgggcgcg ttttgatttg
    13801 cgtgacgtca tcgaccagtc gctgatattg ggtgaggatt tccaacgcat gttcgtaaca
    13861 acgttggcca ctttcagtca gcgccacgcc gcgagcggag cggttcagca gcgttgttgc
    13921 cagcgtattt tcaaggattt ggatccgctt ggtgacaaat gcaggcgtct gcccaagcgt
    13981 cgccgctgcg gcgctaaaac tcccgctgtg aacgatttct accagtacct gcaagtcttt
    14041 ggctaaaggc cagctattca gcatcgttaa ttatctgcag ttgtgataag cgcagtgtat
    14101 ttcgcaaaac attgccattc cctgctctgg cgcaatattc attcttattt tcgcgttgat
    14161 tcacgaactg ttaattacat attcacggtg gcaaaaaata taaaaccaca tttttagtgg
    14221 tagtttgtgg cggtgaattt ttccagacaa atacaaaaac tggagttgcc atgatgagcg
    14281 aaagtaataa gcaacaggca gtgaataagt tgacagagat tgtcgctaac tttaccgcca
    14341 tgatttctac ccgaatgcct gatgacgtgg tggataaact aaaacagcta aaggatgccg
    14401 aaacgtcgtc gatggggaaa attatctacc atacgatgtt cgacaacatg caaaaagcga
    14461 ttgacctgaa tcgtcctgcc tgtcaggaca ccggggagat tatgttcttc gttaaagtcg
    14521 gttcccgctt cccactgctt ggcgagctgc aaagcatact caaacaagcc gtggaagagg
    14581 caaccgtcaa agcgccacta cgtcacaatg cggtagaaat ttttgacgaa gtaaacaccg
    14641 gcaaaaatac cggtagcggc gtaccgtggg tcacctggga catcatcccc gacaatgacg
    14701 atgcggaaat cgaagtttac atggcaggcg gcggctgcac gctacctggc cgctcgaaag
    14761 tgttaatgcc gtcagaaggc tacgaaggcg tggtgaaatt cgtcttcgaa aatatctcca
    14821 ccctcgccgt aaacgcctgt ccaccggtac tggtgggcgt gggcatcgcc acctcggtgg
    14881 aaaccgccgc cgtactctcg cgtaaagcca ttttgcgccc gattggcagc cgccatccca
    14941 atccaaaagc ggcagaactg gagctacgcc tggaagaagg actcaaccgt ctggggattg
    15001 gtccacaagg gctgaccggc aacagttcag tgatgggcgt acatatcgaa tctgccgccc
    15061 gccatccgtc aaccatcggc gttgctgttt ctaccggctg ctgggcgcat cgtcgcggca
    15121 cgctgctggt tcatgccgat ctctcctttg aaaatctgtc tcacacccgg agcgcgttat
    15181 gaaaaagatc ctgacaaccc cgatcaaagc tgaagatctg caagatattc gcgtcggcga
    15241 tgtgatctac ctgaccggta cgctggtgac ctgccgcgac gtttgtcacc gccgcttgat
    15301 cgaactcaaa cgtccgatcc cttacgatct caacggcaaa gcgattttcc acgctggccc
    15361 catcgtgcgt aaaaacggcg ataaatggga gatggtttcc gttggcccga caaccagtat
    15421 gcgtatggaa agttttgaac gtgaatttat cgagcagacc ggcgtgaaac tggtggttgg
    15481 caaaggtggt atggggccgc tgaccgaaga aggctgccag aaattcaagg cgctacatgt
    15541 gattttcccg gcaggctgcg cggtgctggc ggcaacccag gtagaagaga ttgaagaagt
    15601 gcactggaca gaactcggga tgccggagtc actgtgggtc tgccgggtca aagagttcgg
    15661 cccgctgatt gtctctattg atacccacgg caacaacctg atagccgaaa acaaaaagct
    15721 gttcgccgaa cgccgcgatc ccatcgtgga agagatctgc gagcacgtcc attacatcaa
    15781 ataaccctcc cggagaggct caccccctct cctttttcgc aggcataaca cgatgaaacc
    15841 ttccactgaa tggtggcgat accttgcgcc gctggcggtc atcgccatta ttgctctaat
    15901 tccggttccc gcagggctgg agagtcatac ctggctctac tttgccgttt ttactggcgt
    15961 gatcgttgga ctgatcctcg aacccgtgcc gggtgccgtg gtggcgatgg tgggtatatc
    16021 catcatcgcc atactctctc cctggctgct gttcagcccg gagcagctcg ctcagccagg
    16081 ctttaaattc actgcaaaat ccctctcgtg ggccgtttcc ggtttttcta attcggttat
    16141 ctggctgatt ttcgccgcct ttatgtttgg cacaggctat gaaaaaaccg ggcttggacg
    16201 ccgcattgcg ctgattctgg tgaaaaagat ggggcatcgt acgctgtttc tcggctatgc
    16261 ggtgatgttc tccgagctga tcctggcacc tgtaacaccg tccaactcgg cgcgtggtgc
    16321 ggggattatc tatcccatca tccgtaacct gccaccgctc tatcaatcac aaccaaacga
    16381 cagcagttca cgcagcattg gctcgtacat catgtggatg gggattgttg ccgactgcgt
    16441 gaccagcgcc attttcctga cggcgatggc accaaacttg ctgttaattg gactgatgaa
    16501 aagtgcatct cacgccacgc tgagttgggg cgactggttc ctcgggatgt tgccgctcag
    16561 cattttactg gttctgctgg ttccctggat ggcttacgtg ctgtacccac cggtactgaa
    16621 gtctggtgat caggtgccgc gctgggcaga gacggaactg caggcaatgg gcccgctctg
    16681 ttctcgtgaa aaacggatgc tggggctgat ggtaggcgcg ctggtgctgt ggattttcgg
    16741 cggtgattat attgatgctg cgatggttgg ttacagcgta gtggcactga tgctgcttct
    16801 gcgcattatc tgctgggacg acattgtcag taataaagcg gcgtggaacg ttttcttctg
    16861 gctggcctcg cttatcaccc tcgctaccgg actcaacaac accggtttta ttagctggtt
    16921 tggcaaactg ttagcaggca gcttaagtgg ttattcgccg acgatggtga tggtggcatt
    16981 gattgtggtg ttttatctac tgcgctactt tttcgccagc gccacggcgt atacctccgc
    17041 cctcgcaccg atgatgatcg ccgccgcgct ggcgatgccg gaaatcccgc tgccggtgtt
    17101 ctgcctgatg gttggcgcgg caattggtct ggggagcatt cttacaccat acgccaccgg
    17161 acccagtccg atttactacg gtagtggtta tctgccaacg gcggattact ggcgactggg
    17221 ggcgattttt gggctgatat tcctcgtatt gctggtgatt accggcttac tgtggatgcc
    17281 cgtggtgttg ctttaagtaa ggacggtgcc ggaaattgct ctccggcacc cgatagtttt
    17341 acgcggccgg taactccgcc agcggccagc gcggacgcac gctaacgccg agatccgccg
    17401 tcgcgcctgc tttaaaccgc accattccgg cataggcgat catcgcgccg ttatcagtac
    17461 aaaactccgg acgcgcgtag aacacttcgc cgcggcgttt tttcatcatt tcagccagct
    17521 tcgcccgtaa cgtacggtta gcactcacgc cgcctgccat gaccagtcgc ttaaagccag
    17581 tctgatccaa cgctcgcttg cacttaatca tcagcgtatc gaccaccgca tcttcaaagg
    17641 cgcgggcgat atcagcacgc gtctggtcgt cggtgccgtt gtcacgaatg gtatttgccg
    17701 cgaaggtttt cagaccggag aagctgaaat ccagccccgg acggtcggtc atcggacgcg
    17761 ggaagacaaa gcgcccggca gtaccctgag ccgccatttt cgacagtaac ggtccgccag
    17821 gataatccag ccccagcagc ttcgcggttt tatcaaacgc ttcaccggcg gcatcatcga
    17881 tagactcgcc gagcagctcg tactgaccaa tgccagtcac gctgattaac tgcgtatgac
    17941 cgccggacac cagcagcgcg acaaacggaa attccggcgg gttatcttcc agcatcggcg
    18001 ctaacagatg cccttccata tggtgtaccg ggattgccgg aacgttccag gcaaacgcca
    18061 gagaacgccc cacggttgcg ccaaccagca gtgcgccgac taatccaggg cctgcggtat
    18121 aggccacagc atcaatgtct tttgccgtta agccagactc ctttagcgcc gcctggatca
    18181 acggtacggt tttgcgcacg tgatcgcggg aggccagttc aggcacgacg ccgccgtagt
    18241 cagcgtgcaa tttcacctga ctatacaatt ggttggctaa caaacctttt tcatcgtcgt
    18301 aaatggcgat gccggtttca tcgcaggaag tttcaatacc cagtacacgc atgacttgtt
    18361 ttacctcgct ttattaccgc gcagtgtagg accaatgcgg gttgatgtaa aactttgttc
    18421 gcccccggag aaagcctcgt gtatactcct cacccttata aaagtccctt tcaaaaaggc
    18481 cgcggtgctt tacaaagcag cagcaattgc agtaaaattc cgcaccattt tgaaataagc
    18541 tggcgttgat gccagcggca aaccgaatta atcaaaggtg agaggcacat gccggtaatt
    18601 aaagtacgtg aaaacgagcc gttcgacgta gctctgcgtc gcttcaagcg ttcctgcgaa
    18661 aaagcaggtg ttctggcgga agttcgtcgt cgtgagttct atgaaaaacc gactaccgaa
    18721 cgtaagcgcg ctaaagcttc tgcagtgaaa cgtcacgcga agaaactggc tcgcgaaaac
    18781 gcacgccgca ctcgtctgta ctaattcccc gagagcgttg ctctccgatc agaccgagtt
    18841 gtagttgtaa ggccgtgctt ccgaaaggaa tgcgcggctt attttcgttt atgaattgct
    18901 aaaaatcggg gcctatggct ggacgaatcc cacgcgtatt cattaatgat ctgctggcac
    18961 gcactgacat cgtcgatctg atcgatgccc gtgtgaagct gaaaaagcag ggcaagaatt
    19021 tccacgcgtg ttgtccattc cacaacgaga aaaccccgtc cttcaccgtt aacggtgaga
    19081 aacagtttta ccactgcttt ggatgtggcg cgcacggcaa cgcgatcgac ttcctgatga
    19141 actacgacaa gctcgagttc gtcgaaacgg tcgaagagct ggcagcaatg cacaatcttg
    19201 aagtgccatt tgaagcaggc agcggcccca gccagatcga gcgccatcag aggcaaacgc
    19261 tttatcagtt gatggacggt ctgaatacgt tttaccaaca atctttacaa caacctgttg
    19321 ccacgtctgc gcgccagtat ctggaaaaac gcggattaag ccacgaggtt atcgctcgct
    19381 ttgcgattgg ttttgcgccc cccggctggg acaacgtcct gaagcggttt ggcggcaatc
    19441 cagaaaatcg ccagtcattg attgatgcgg ggatgttggt cactaacgat cagggacgca
    19501 gttacgatcg tttccgcgag cgggtgatgt tccccattcg cgataaacgc ggtcgggtga
    19561 ttggttttgg cgggcgcgtg ctgggcaacg atacccccaa atacctgaac tcgccggaaa
    19621 cagacatttt ccataaaggc cgccagcttt acggtcttta tgaagcgcag caggataacg
    19681 ctgaacccaa tcgtctgctt gtggtcgaag gctatatgga cgtggtggcg ctggcgcaat
    19741 acggcattaa ttacgccgtt gcgtcgttag gtacgtcaac caccgccgat cacatacaac
    19801 tgttgttccg cgcgaccaac aatgtcattt gctgttatga cggcgaccgt gcaggccgcg
    19861 atgccgcctg gcgagcgctg gaaacggcgc tgccttacat gacagacggc cgtcagctac
    19921 gctttatgtt tttgcctgat gacgaagacc ctgacacgct gatacgaaaa gaaggtaaag
    19981 aagcgtttga agcgcggatg gagcaggcga tgccactctc cgcatttctg tttaacagtc
    20041 tgatgccgca agttgatctg agtacccctg acgggcgcgc acgtttgagt acgctggcac
    20101 tgccattgat atcgcaagtg ccgggcgaaa cgctgcgaat atatcttcgt caggaattag
    20161 gcaacaaatt aggcatactt gatgacagcc agcttgaacg attaatgcca aaagcggcag
    20221 agagcggcgt ttctcgccct gttccgcagc taaaacgcac gaccatgcgt atacttatag
    20281 ggttgctggt gcaaaatcca gaattagcga cgttggtccc gccgcttgag aatctggatg
    20341 aaaataagct ccctggactt ggcttattca gagaactggt caacacttgt ctctcccagc
    20401 caggtctgac caccgggcaa cttttagagc actatcgtgg tacaaataat gctgccaccc
    20461 ttgaaaaact gtcgatgtgg gacgatatag cagataagaa tattgctgag caaaccttca
    20521 ccgactcact caaccatatg tttgattcgc tgcttgaact gcgccaggaa gagttaatcg
    20581 ctcgtgagcg cacgcatggt ttaagcaacg aagaacgcct ggagctctgg acattaaacc
    20641 aggagctggc gaaaaagtga tttaacggct taagtgccga agagcgatcg ggaagccccc
    20701 gacagccgca ctgagaggca gcggcaaata tataagtacg ccctcgtaat tatcgttggc
    20761 ggtaaacaac cgttggattt cagcgttaac ggctgaagga catcgggtca atcgcccaac
    20821 accaacctca tgaaataagt gtggataccg tcttatggag caaaacccgc agtcacagct
    20881 gaaacttctt gtcacccgtg gtaaggagca aggctatctg acctatgccg aggtcaatga
    20941 ccatctgccg gaagatatcg tcgattcaga tcagatcgaa gacatcatcc aaatgatcaa
    21001 cgacatgggc attcaggtga tggaagaagc accggatgcc gatgatctga tgctggctga
    21061 aaacaccgcg gacgaagatg ctgccgaagc cgccgcgcag gtgctttcca gcgtggaatc
    21121 tgaaatcggg cgcacgactg acccggtacg catgtacatg cgtgaaatgg gcaccgttga
    21181 actgttgacc cgcgaaggcg aaattgacat cgctaagcgt attgaagacg ggatcaacca
    21241 ggttcaatgc tccgttgctg aatatccgga agcgatcacc tatctgctgg aacagtacga
    21301 tcgtgttgaa gcagaagaag cgcgtctgtc cgatctgatc accggctttg ttgacccgaa
    21361 cgcagaagaa gatctggcac ctaccgccac tcacgtcggt tctgagcttt cccaggaaga
    21421 tctggacgat gacgaagatg aagacgaaga agatggcgat gacgacagcg ccgatgatga
    21481 caacagcatc gacccggaac tggctcgcga aaaatttgcg gaactacgcg ctcagtacgt
    21541 tgtaacgcgt gacaccatca aagcgaaagg tcgcagtcac gctaccgctc aggaagagat
    21601 cctgaaactg tctgaagtat tcaaacagtt ccgcctggtg ccgaagcagt ttgactacct
    21661 ggtcaacagc atgcgcgtca tgatggaccg cgttcgtacg caagaacgtc tgatcatgaa
    21721 gctctgcgtt gagcagtgca aaatgccgaa gaaaaacttc attaccctgt ttaccggcaa
    21781 cgaaaccagc gatacctggt tcaacgcggc aattgcgatg aacaagccgt ggtcggaaaa
    21841 actgcacgat gtctctgaag aagtgcatcg cgccctgcaa aaactgcagc agattgaaga
    21901 agaaaccggc ctgaccatcg agcaggttaa agatatcaac cgtcgtatgt ccatcggtga
    21961 agcgaaagcc cgccgtgcga agaaagagat ggttgaagcg aacttacgtc tggttatttc
    22021 tatcgctaag aaatacacca accgtggctt gcagttcctt gacctgattc aggaaggcaa
    22081 catcggtctg atgaaagcgg ttgataaatt cgaataccgc cgtggttaca agttctccac
    22141 ctacgcaacc tggtggatcc gtcaggcgat cacccgctct atcgcggatc aggcgcgcac
    22201 catccgtatt ccggtgcata tgattgagac catcaacaag ctcaaccgta tttctcgcca
    22261 gatgctgcaa gagatgggcc gtgaaccgac gccggaagaa ctggctgaac gtatgctgat
    22321 gccggaagac aagatccgca aagtgctgaa gatcgccaaa gagccaatct ccatggaaac
    22381 gccgatcggt gatgatgaag attcgcatct gggggatttc atcgaggata ccaccctcga
    22441 gctgccgctg gattctgcga ccaccgaaag cctgcgtgcg gcaacgcacg acgtgctggc
    22501 tggcctgacc gcgcgtgaag cgaaagttct gcgtatgcgt ttcggtatcg atatgaacac
    22561 cgaccacacg ctggaagaag tgggtaaaca gttcgacgtt acccgcgaac gtatccgtca
    22621 gatcgaagcg aaggcgctgc gcaaactgcg tcacccgagc cgttctgaag tgttgcgtag
    22681 cttcctggac gattaatcgg taggccggat caggcgttac gccgcacccg gcactagtcc
    22741 ctctgcacaa acgccacctt ttcggtggcg ttttttatcg cccacgcact accagcgcct
    22801 ggtccagctc gcgatacgct tcaaccagtt tctctagtga aacgcgactt aaaccgctgg
    22861 gatttggcag cacccaaatc tgcgtcgaac caatggtgag cgtttgtttc ccccactgtg
    22921 caccgcgctg gctgaatccc tgttcatatg cttgtttgcc cagaatcgcc aacgcctgcg
    22981 gctgataatc ctcaattttt tcaatcaact tacgcccacc tgcgtgcaac tcctgctttg
    23041 aaacttcact ggcttgcacc gttggcctgt caaccagttt ggtgacgcca caacgataat
    23101 ccagcagatg ctgtgcctcc tgcggcttca actgacggtc ggtaaacccg gcctgatata
    23161 tcaccttcca gaagcgattt gccggatgag caaagggaaa accagtcccg gcggatgaaa
    23221 gcccagggtt gataccgcaa aacacgaccc gtaaccctgg agccaaaata tcctcaacca
    23281 tatttactcc tttctaacat caccaggaaa agtataacgg attgaaaata cattgtttat
    23341 aaaaacagca ggcgcgcggt aatggctgga ttgcgacacg gagttacttt ataatccgct
    23401 accatggccc cttagctcag tggttagagc aggcgactca taatcgcttg gtcgctggtt
    23461 caagtccagc aggggccacc agatatagca aaggctgacg agaaatcgtc agcctttttc
    23521 ttttacatat cagttacttt gcgtgccagt aggccgctgc acgaacccgc tgtgggtcaa
    23581 actgttccgt ttcaaagcgg cggcttaaat tcttaacgac tttgccttcg ccggttatcc
    23641 agatgaagta atcatcggca gggatttgca tctgcgccag acgcgcatct accgcctgct
    23701 catcatgtgc cagccattcg atattaaaac catcaaggtg cgcgagataa tcctgacagg
    23761 cgttatcccg cacgctaacc agcacgctaa cttgcggttt aacggcaagt ttgctcaacg
    23821 tttccaggcg gcggcgcaat gcgggcattc cggattcatc gcagacatac agctgatacg
    23881 cgtaatcttc cggcaccacc agcgaaccgc gcggacctgc caccgtgagt ttatcgcccg
    23941 gttgggcctg cattgcccag ctactggcga cgccgtcttc gtgaataaag aaatcaatcg
    24001 ccagttcatg gcgtagttcg tcatacaacg gcgtatagtc acgcgacggt gggcgtggtc
    24061 cttccggcca gacgatgccc tcttccgtta ccgttggcgg cacaaagtga gcgtcaggtt
    24121 gaggaaagaa gagtttgctg tgatcgtcaa agccgcgcga tgtaaaaccg tccagcgcct
    24181 cgccgccgag gacaatgcgc tgaaaaccgg cgctgatgcg ctcaacgcgt aacacagtca
    24241 gttcacggaa tcgcagatca ttgcgaacgc gctgcgggta gcggggggag ttattcattg
    24301 ttatcgcctt cgtgatggta attagatata tctaaataaa actcgc
//
LOCUS       DAPHBJ010000055        24120 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.055, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000055 DAPHBJ010000000
VERSION     DAPHBJ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.055"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            22..432
                     /gene="gspS2"
                     /locus_tag="RHZ76_001951"
     CDS             22..432
                     /gene="gspS2"
                     /locus_tag="RHZ76_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HDV2812600.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTLKMTIISEAGTQTTQTPDAFLTSYQRQMCTDPTVKLM
                     LTEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            450..1175
                     /locus_tag="RHZ76_001952"
                     /pseudo
     CDS             450..1175
                     /locus_tag="RHZ76_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="part of the general (type II) secretion pathway
                     (GSP), a signal sequence-dependent process responsible for
                     protein export; involved in trans-location of exoproteins
                     from the cytoplasm to the periplasm; incomplete; partial
                     in the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
     gene            1226..1762
                     /gene="yghD"
                     /locus_tag="RHZ76_001953"
     CDS             1226..1762
                     /gene="yghD"
                     /locus_tag="RHZ76_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HDV2812601.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETILQQLVAMNTRLKNAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIRRIADRGENIQVWIEPVVFNDLLKWLNALDEKYALRVTQIDVS
                     AAEKPGMVNVQRLEFGRG"
     gene            complement(1911..1986)
                     /locus_tag="RHZ76_001954"
     tRNA            complement(1911..1986)
                     /locus_tag="RHZ76_001954"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1951..1953),aa:Phe,seq:gaa)
     gene            complement(2092..2786)
                     /locus_tag="RHZ76_001955"
                     /pseudo
     CDS             complement(2092..2786)
                     /locus_tag="RHZ76_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
     gene            3184..5319
                     /gene="speC"
                     /locus_tag="RHZ76_001956"
     CDS             3184..5319
                     /gene="speC"
                     /locus_tag="RHZ76_001956"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDV2812602.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPYR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVEIGIEARKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLVE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGDVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGVKRLYGYVLK"
     gene            complement(5369..6625)
                     /gene="nupG"
                     /locus_tag="RHZ76_001957"
     CDS             complement(5369..6625)
                     /gene="nupG"
                     /locus_tag="RHZ76_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HDV2812603.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6827..7906)
                     /gene="mltC"
                     /locus_tag="RHZ76_001958"
     CDS             complement(6827..7906)
                     /gene="mltC"
                     /locus_tag="RHZ76_001958"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HDV2812604.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7971..8246)
                     /locus_tag="RHZ76_001959"
     CDS             complement(7971..8246)
                     /locus_tag="RHZ76_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HDV2812605.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(8274..9326)
                     /gene="mutY"
                     /locus_tag="RHZ76_001960"
     CDS             complement(8274..9326)
                     /gene="mutY"
                     /locus_tag="RHZ76_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDV2812606.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWAL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9487..10206
                     /gene="trmB"
                     /locus_tag="RHZ76_001961"
     CDS             9487..10206
                     /gene="trmB"
                     /locus_tag="RHZ76_001961"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDV2812607.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            10206..10532
                     /locus_tag="RHZ76_001962"
     CDS             10206..10532
                     /locus_tag="RHZ76_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HDV2812608.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            10716..11435
                     /locus_tag="RHZ76_001963"
     CDS             10716..11435
                     /locus_tag="RHZ76_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HDV2812609.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11611..12657
                     /gene="ansB"
                     /locus_tag="RHZ76_001964"
     CDS             11611..12657
                     /gene="ansB"
                     /locus_tag="RHZ76_001964"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDV2812610.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12774..13781
                     /locus_tag="RHZ76_001965"
     CDS             12774..13781
                     /locus_tag="RHZ76_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HDV2812611.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13936..15072)
                     /gene="hemW"
                     /locus_tag="RHZ76_001966"
     CDS             complement(13936..15072)
                     /gene="hemW"
                     /locus_tag="RHZ76_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HDV2812612.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRAEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(15065..15658)
                     /locus_tag="RHZ76_001967"
     CDS             complement(15065..15658)
                     /locus_tag="RHZ76_001967"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HDV2812613.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(15666..15956)
                     /locus_tag="RHZ76_001968"
     CDS             complement(15666..15956)
                     /locus_tag="RHZ76_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HDV2812614.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(15953..16519)
                     /gene="yggT"
                     /locus_tag="RHZ76_001969"
     CDS             complement(15953..16519)
                     /gene="yggT"
                     /locus_tag="RHZ76_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HDV2812615.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(16537..17241)
                     /gene="yggS"
                     /locus_tag="RHZ76_001970"
     CDS             complement(16537..17241)
                     /gene="yggS"
                     /locus_tag="RHZ76_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HDV2812616.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17259..18239
                     /locus_tag="RHZ76_001971"
     CDS             17259..18239
                     /locus_tag="RHZ76_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDV2812617.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGMILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHRVGEGRL"
     gene            complement(18423..18839)
                     /gene="ruvX"
                     /locus_tag="RHZ76_001972"
     CDS             complement(18423..18839)
                     /gene="ruvX"
                     /locus_tag="RHZ76_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDV2812618.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(18839..19402)
                     /locus_tag="RHZ76_001973"
     CDS             complement(18839..19402)
                     /locus_tag="RHZ76_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HDV2812619.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(19511..20461)
                     /gene="gshB"
                     /locus_tag="RHZ76_001974"
     CDS             complement(19511..20461)
                     /gene="gshB"
                     /locus_tag="RHZ76_001974"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HDV2812620.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(20474..21205)
                     /gene="rsmE"
                     /locus_tag="RHZ76_001975"
     CDS             complement(20474..21205)
                     /gene="rsmE"
                     /locus_tag="RHZ76_001975"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDV2812621.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(21285..21992)
                     /gene="endA"
                     /locus_tag="RHZ76_001976"
     CDS             complement(21285..21992)
                     /gene="endA"
                     /locus_tag="RHZ76_001976"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HDV2812622.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(22087..22584)
                     /locus_tag="RHZ76_001977"
     CDS             complement(22087..22584)
                     /locus_tag="RHZ76_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HDV2812623.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MENVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(22661..24055)
                     /gene="galP"
                     /locus_tag="RHZ76_001978"
     CDS             complement(22661..24055)
                     /gene="galP"
                     /locus_tag="RHZ76_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HDV2812624.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
BASE COUNT         6004 a         6059 c         6355 g         5702 t
ORIGIN      
        1 taaccttaga gattatttac catgtcgata aaacagatgc cagggagggt attaatatcg
       61 ctattgttga gcgttacagg attattaagt ggctgtgcca gccataatga aaacgccagt
      121 ttactggcga aaaaacaggc gcaaaatatc agccaaaacc tgccgattaa atctgcggga
      181 tataccttag tgctggcaca aagtagcggc acgacgttaa aaatgaccat tatcagcgaa
      241 gcggggactc agaccacgca gacgcctgac gcctttttaa ccagctatca acgacaaatg
      301 tgcactgacc caacggtgaa attaatgctc actgagggaa ttaattacag cataacgatt
      361 aatgatacac gtacaggtaa ccagtatcag cggaaactgg atcgcactac ctgtggaata
      421 gttaaagcat aacgtcgggt agatataaat tggcgcgggt tgtttttcgt gacgcacgaa
      481 tttatctcat tcaatggctg acaaaaattc gtcacactct taaccagaga caatctctta
      541 atacagacaa agagcatctg cgaaaaattg tacgcgggat gttctggctg atgctgctta
      601 ttatttctgc aaaagtggcg cattcactct ggcgctattt ctctttttct gcggaatata
      661 tggctgtttc cccatcggcg aataaaccgc cccgtgcgga tgcaaaaacg ttcgataaaa
      721 atgacgtgca attaatcagc cagcaaaact ggtttggcaa atatcagccc gtcgccacgc
      781 cggtaaaaca acccgaacct gcacctgtgg cagaaacgcg tcttaatgtg gtgttgcgtg
      841 ggatcgcctt tggtgccaga cccggcgcgg ttattgaaga aggtggcaaa cagcaggtct
      901 atttgcaggg ggaacggctt ggttctcaca acgcggtgat tgaggaaatc aatcgcgacc
      961 atgtgatgct gcgctatcag ggaaaaatag agcgcctgag cctggcagaa gaggagcgtt
     1021 ccacggttgc tgtgacccgc caaaaagcca tcagtgacga agcaaagcaa gctgttgctg
     1081 aacctgctgt cagtgcgcca gttgagatcc cgactgccgt gcgtcaggca ctggcgaaag
     1141 atccgcagaa aatttttaac tatatccagc ttacagaaag agggtgatgc ggtttccggc
     1201 ggcgtaacgt tgcggaggaa ataacatgtt acgcgataaa tttattcact attttcagca
     1261 atggcgtgaa cgccagttaa gccgtggcga acactggctg gcacaacacc tggcggggcg
     1321 ttctccgcgt gaaaagggca tgttactggc agcggtggtg ttcctgttta gcgtcggata
     1381 ttacgtcctc atctggcagc cgttgagcga acggattgag caacaggaga caatattgca
     1441 gcagctggtg gcgatgaaca cccgactgaa gaacgccgcg ccggatatta ttgcagcgcg
     1501 aaaatctgcc acaacaacgc ctgcgcaggt atcgcgggtc atcagtgaca gtgcttccgc
     1561 gcactcggtg gtcatcaggc ggatagccga tcgtggggag aatattcagg tctggataga
     1621 acctgtggtg tttaatgacc tgctgaaatg gttaaacgca ctggatgaaa aatatgcgct
     1681 gcgggtgaca caaattgatg tcagtgctgc tgagaagcct gggatggtga atgtgcagcg
     1741 gctggagttt ggacgggggt aaagtatcag gagattaata tcaataaaat tactctcgtt
     1801 aacgtggcga gagtaattcc cccagacttt ataagctaat ttttttcgtg aaggttattg
     1861 tcagatccaa aagcaaaaac ccgccttgtg ggcgggttct taagaattag tggtgcccgg
     1921 actcggaatc gaaccaagga cacggggatt ttcaatcccc tgctctaccg actgagctat
     1981 ccgggcaacg gggcgcatta aacctgattc gcctcgtctc gtcaatcaaa tttcttcaat
     2041 ttactgcaga ctgcacaatc tatcatcact ttgccgctat tgcacgcatt gtcaggcaaa
     2101 ccaggcggcg gaaagcggca ttgccagaag gagtgctgga agcatgttga ccaccgggaa
     2161 cattttaatg ccacagatgc gtaatccggt tgccagcagc aataaaccgc ctacggcgct
     2221 gaagtctgcc atcatcgacg gtgtggtcag cggtaatatc agcgcggcag cccacgccag
     2281 cgttaactgg atgatcagta atgggatact aattaccgac accgcaatac ccagcgagca
     2341 ggcgaagatc atcgccgtaa agaaatcaag aaatgacttg gcgattaaaa tactcggatc
     2401 gccggtcatc ccttcgttca tcgccccgaa gatcccggtg ccgctggcgc aaaacaggac
     2461 aataatcgcg acataattct gaataaaaga ttcatgcgct ggcttcttac gtgagtgacg
     2521 aaacagattt tgtgctttgg cgactgctgt attgacacct ttctccagca aacaaatttc
     2581 gccaattaga gcgccaagta gcgtagctaa aaccatcgct ggaaggttgg cacatttcac
     2641 caccagtaaa ataccaatcc ccagcgatgc cagaccaaaa attgatgtca tggagacgcg
     2701 gatacgttcc ggtaagcgtt ggctgagcag tgcgccgaga acgccaccca gtatgcacta
     2761 gcgttgataa aagggccgat gaccacaaga gttcctgtta gctgtattgt ttgattttct
     2821 tattatgaac tttttgcctt gttgatggct tgtctggtcg caaagtcctg gcttgcacgc
     2881 tttagcgaaa ggtgccatga ttgcgcgaat tttctcctct ctgtacggag tttgcccgat
     2941 gcacgccatc tccttacatt ctctcgctta tcgccgtttc gcgcgaaacg tttccctttc
     3001 tatgttactg ctcatgcggt gaagttaacg cacgctcact gcaggacaac agtaaaatca
     3061 gagcgtttct gcttttactg atgtctggcg gtcggagctg gtgaccagtt tgacccatat
     3121 ctcatggggc agggttttcc accttgtccg gtattcttac ttccccgaaa cgggtttgcg
     3181 cttatgaaat caatgaatat tgccgccagt agtgaactgg tatcccgact ttcttctcat
     3241 cgtcgcgtgg tggcgttggg agatactgat tttacggacg tcgcggcagt cgtcattacc
     3301 gctgcggata gtcgcagtgg cattcttgcg ttgcttaagc gcaccggttt tcatctaccg
     3361 gtgtttttgt attccgaaca tgctgttgaa ttacctgcgg gcgttacggc ggtaatcaac
     3421 ggcaacgagc agcagtggct ggagctggaa tccgcagcct gtcagtatga agagaatttg
     3481 ctgccaccgt tttatgacac gctgacgcag tacgttgaga tgggcaacag cacctttgct
     3541 tgccctggac atcaacatgg tgcgtttttt aaaaagcatc ctgccggacg ccatttttac
     3601 gatttctttg gtgagaacgt ctttcgcgcc gatatgtgta acgctgacgt aaaattgggc
     3661 gatctgctta ttcatgaagg atcggcgaaa gatgcgcaga aattcgcagc caaagtcttt
     3721 catgccgata aaacctattt tgtgctgaac ggcacatcgg cagcgaataa agtggtgacg
     3781 aatgcgctgt taacgcgtgg cgatctggtg ctcttcgacc gtaacaacca taagtcgaat
     3841 catcacggcg cgctgattca ggcgggggcg acgccggtct atctggaagc ttcacgcaac
     3901 ccgtttggtt tcattggcgg tattgatgcg cactgtttta atgaagagta tctgcgccag
     3961 caaattcgcg acgttgcgcc agaaaaagcc gacctgccgc gcccgtatcg cctggcgatt
     4021 attcagctgg gaacctatga cggcactgtc tataacgccc gtcaggtgat cgataccgtt
     4081 gggcatctgt gtgattacat tctgtttgat tccgcgtggg tcggttatga acaatttatc
     4141 ccgatgatgg cggatagctc gccgctgctg ttagaactta acgaaaacga tccggggatc
     4201 tttgtgactc agtcggtgca caaacagcag gcgggattct cacagacgtc gcagatccat
     4261 aaaaaagata accatatccg cggacaggcg cgtttttgcc cgcataagcg gttgaataac
     4321 gcctttatgc tccatgcttc taccagccct ttctatccgc tgtttgctgc actggatgtt
     4381 aacgccaaaa ttcatgaagg ggagagtggg cgtcggctgt gggctgagtg tgttgagata
     4441 gggattgaag cgcgcaaggc tattcttgcg cgctgtaagc tgttccgccc gtttatcccg
     4501 cccgttgttg atggcaaatt gtggcaggat tatccgacat cagtgttagc cagcgaccgc
     4561 cgttttttca gttttgagcc gggggcgaag tggcacggct ttgaaggata tgccgcggat
     4621 cagtattttg ttgatccgtg caagctgtta ctcactacac caggtatcga tgccgaaacc
     4681 ggcgaatata gcgactttgg cgttccggcg acgattctgg cgcactatct gcgtgagaac
     4741 ggcattgtgc cggagaagtg cgatctcaac tccattctgt ttttattaac tccggcggaa
     4801 agccacgaga agctggcaca actggtggcg atgctggcgc aatttgaaca gcatattgag
     4861 gatgactcgc cgctggttga ggtgttgccg agcgtttata acaagtatcc ggtgcgctat
     4921 cgcgactaca ccctgcgcca gttgtgtcag gagatgcacg atctgtatgt cagtttcgac
     4981 gtcaaagacc tacaaaaagc gatgttccgc cagcagagtt tcccgtcagt ggtgatgaac
     5041 ccccaggatg cgcatagcgc ttatattcgc ggtgacgtgg agttggtgcg gattcgtgat
     5101 gccgaagggc gaattgcggc agaaggggcg ttgccttatc cacctggcgt gctttgcgtg
     5161 gtacccgggg aagtctgggg tggggcggtt caacgttatt tccttgcact ggaagaaggg
     5221 gtgaatttgt tgccgggatt ttcgccggag ctgcaaggtg tttatagcga aaccgatgcg
     5281 gatggcgtga aacggttgta cggttatgtg ttgaagtaag aataaaaaaa acgggtcacc
     5341 ttctggcgac ccgtttttct ttgcgtaatt agtggctaac cgtctgtgtg cctgtcggga
     5401 cacgaacgtg tttatatttg aacatcgcca tgaacgcgaa ggccagaacc acggagtaac
     5461 cagcgaaaat caaccatacg gtctgccagt cggtaatgcc gttttgggtg tacatctcaa
     5521 caactttacc gctcacgatg ccgccgagga tacagccgaa gccgttagtc atcatcagga
     5581 acatcccttg tgcactggcg cgaattgccg ggctaacttc tttttcgaca aacaccgaac
     5641 cagagatgtt gaagaagtcg aatgcgcaac cgtaaacgat catcgacagt accagcagta
     5701 cagtaccgaa cggagtcggg tcgccgtaag caaacagcgc aaaacgcagg atccacgcca
     5761 caatactgat catcattacg ttcttaatac cgtagcggct taagaagaac gggatggtca
     5821 gaatgaacag ggtttcagag atctgcgaaa tcgacatgat gattgacgca tgctgcacaa
     5881 taaagctgct ggcaaacatc ggatctttgt cgaagctgtg caggaaggta ttaccgaaca
     5941 tgttggtaat ctgcagttcc gcgcccagca gcattgagaa gataaagaag attgccatac
     6001 gcttgttttt aaacagcgcg aatgcatcga ggcccagcag ggttgtccag ctctgattcg
     6061 cttgctgttt agcaaccgga atatgcggca gagtcagggt aaacagaacc agaatggcgg
     6121 aaagtgctgc gccaatatac agctgcatgt ggcttaattc gaagccagac aggctcacca
     6181 cccacattgc catgataaag ccgatggtgc cccagatacg gattggcggg aagtcagtaa
     6241 cgatatccat cccggcattt tgcaggcgat agtaagagat ggtgttgatt aacccaagcg
     6301 ttggcatata agcaaacgag ttaatcaata tcacaaggaa catcgcttcc ggtgtcgtga
     6361 cctgtgccgc catgaacagc gtgatagcgc caatggtgtg gcaaatggca tatacccatt
     6421 tcgcacttaa ccatttgtcg gccacaatcc ccagcagcgc aggcataaag accgctgcga
     6481 tacccagtga gctataaact gcgccaatag aagcaccgtc aaacttcagg gtaacaaaca
     6541 tataggagcc gagggtcgtc agccaacttc cccacagaca gaactgcaga aaagagagga
     6601 ttttcagctg cagcttaaga ttcatgttaa tttcctcaca tcgtgatgcg gatgagtgtt
     6661 tgaaaaagca catttgaccg tgcgagatga tgggattcta tcaacttcgc ggactggttt
     6721 tttgttacct gtggcaaata attgcaaaca ttcccaattt gcaaaacgaa attgtgatgt
     6781 ggataacatt tttgcccctg agcatcgtca ggggcggtta atggaattat cggcggcggt
     6841 aggatttttg cgcggtattc actttataaa gataacggcg agattccgca gagggatggc
     6901 gggtcgtcag cgtctgataa acatcgcccg gcgtcatggt gttaataata ttggcagcct
     6961 gaatcttatc attcgaaaag actcgcagca cgctgcctgc gccgccgtta taggcggtga
     7021 tgacggcata acgccgcgat gttgggttat caattccgcc tagataaaca ttgttcagca
     7081 tcgccagata cgcggtgccg gtatcaatat tgctggcagg atcaaacaag aaactgcggc
     7141 tcggcgtgcc ggatttcccc tgcgagcgga acacatcttt cccggcagta tgttgtacca
     7201 cctgcattaa tcccagcgca tcggaacggc tgaccgcata cgggttaaag gaagattcgg
     7261 tctgcataat tgccagaatc agcgactcat caacgccata tttccgtgac gcctggcgga
     7321 ccatgccgag atatttgtgc gcacgtttat caaggtggtt cggtaccatg ttaatggtga
     7381 cgctgtagat gatacgcagc ccgttactgc ggctttgcag acggtttttc agcagataat
     7441 ccgcgaagtt gcttgcgcga ccttcccagc gaatcggctg cccggtgttg tccaccacct
     7501 gaccgtaaag gaaaggttct ttcgaaatcg taatatcatc aacgtcggaa tagagatcga
     7561 ccgaactcgg atcgtcaccc atcagcaacg ttttgataat tgcccggcgc aaatgcgcgg
     7621 caggttctgt cccggcgata gtttcgatag taatcgtacc gtcatcgaag ttgatgtggc
     7681 tgcgggtctg atattgatcg gtgtatttca cgtagtcctt aggaccggcg atcaccacct
     7741 ctttgaatcc ccagatgttc tcaatattgt gggcaaattg ccccatcaga atatcaaaac
     7801 cgttggtatc tttgacccag gcttcgttat aggtatcgcc ttttttggtc gtcgaacagg
     7861 agatgagcaa cggcgcaatc aaagccagcg cgagatattt tttcatcatt ccgggtgcgt
     7921 gttgtgtttg tttttatgca agttgccgga ggcgtgctcc ggcactgttt ttatttttta
     7981 tcttccggcg tatagccctc gatatgcacc tctttaccct cgaacaggaa gttgaccatc
     8041 tcctgctcaa gcagcttgcg gtgctcggca ttcatcatgt tgagtttctt ttcattaatc
     8101 agcatggttt gcttgtgctg ccactgcgcc caggcttctt tggagatctc gttatagatg
     8161 cgttttccca gctcgccggg gtacagctga aaatcctgac cttctgcttc acgttgcagg
     8221 aaagtacaaa aaatcgttct gctcataaat catcctcttt atcgactcac gcgctaaacc
     8281 ggcgcgccag tgcgtaactg ctgtaacaaa cgctccacgg gagccgccag gccaactgac
     8341 ggcggttgcg ctaagttata ccagagcgca ttgccttcat ccatgcagcc ggtgaatgac
     8401 gacacgggaa gccacatagg cacaatatct aagtggaaat ggctgaaggt atgccgaaac
     8461 gcggtcagtt gcgtcaggtt atcggcagca atctgccgtt gcgccagcca ctgccgcaaa
     8521 ctttcttcgt cggcaaactg cgggaaacag tataaaccgc cccacaatcc gctcggcgga
     8581 cgctgcgcca gcaatacttc atcttcgtgc tgtaatagca aaaagtagcc ggtgcgctcc
     8641 ggcagcgtct gtttcggttt tttgcccgga taaagcgccc agctattgtt ggcggcggca
     8701 atacatccgt tttgtagcgg acagagcgaa catttcggtt tcgagcgcgt acaaatcatc
     8761 gcacccaaat ccatcatcgc ctgattaaac cgttccacgc caaccgcggg cgtcacctgc
     8821 tcgctcaaac tccataattt attctcgacc tcttttttcc caggccagcc gcttacagca
     8881 tagcagcgcg ccagcacgcg tttgacgtta ccgtcgagaa tcggaaagtg cttacccaga
     8941 gaaagcgaga gaatcgcgcc tgcggtggaa cgcccgacgc ccggtaacgc cgcgacttct
     9001 tcaaaggttt ccgggaattt accgccgtgt aaggtcgcca cttgttgtgc cgctttatgc
     9061 agattgcgcg cgcgggcgta atagccaagc ccggtccaca agtggagaac ttcgtcgagc
     9121 ggcgcattgg cgagatcggt caccgtcggg aagcgcgcca taaagcgttc aaaatagggg
     9181 ataacggtcg caacctgagt ttgttgcaac atcacttctg agagccatac tttgtagggc
     9241 gtcttgtcaa tttgccaggg cagcgttttt cgcccgtatt tatcgtacca gtccagaacc
     9301 tgggctgaaa attgcgacgc ttgcatggtc accgaattca ctgttgttgg gggcaagatt
     9361 gcagcacagc gacggcaggg tgtaaaccgg aactttccgc agccacggcc ttatcatgct
     9421 tgcatccggc aactaacttt ggataatgcc cgttttcaga acactttcac aagcgactaa
     9481 atctttatga aaaacgacgt catttcaccg gaatttgatg aaaacggccg cccactgcgc
     9541 cgtatccgta gttttgtgcg ccgccagggg cgactgacca aaggccagga acatgcgctg
     9601 gaaaactact ggccggtgat gggcgttgag ttcagcgaag atatgctgga tttccccgcg
     9661 ctttttggcc gtgaagcgcc ggtgacgctt gagattggtt ttggcatggg ggcgtcgctg
     9721 gtggcaatgg ctaaagatcg ccctgagcag gacttcctcg gcattgaagt gcattcaccg
     9781 ggcgttggtg cgtgcctggc ttctgcgcat gaagaaggtt taagcaacct gcgagtgatg
     9841 tgtcacgatg cggttgaagt gctgcataaa atgattcctg acaattcatt gcgcatggtg
     9901 cagctctttt tccctgaccc gtggcacaaa gcgcgccata ataaacgccg tatcgtacag
     9961 gtgccgtttg ccgaactggt aaaaagcaaa ctgcagctgg ggggggtatt ccatatggcg
    10021 accgactggg aaccttatgc ggaacatatg cttgaagtga tgtcttctat tgacggttat
    10081 aaaaacctgt cagagagcaa tgattacgta ccgcgtccgg catcacgtcc ggtgacgaaa
    10141 tttgaacaac gtggtcatcg tcttggtcac ggagtatggg acttaatgtt cgagagggtg
    10201 aaataatggc aaagaaccgt agccgtcgtc tgcgtaaaaa aatgcacatc gacgaattcc
    10261 aggaattagg attttcggtg gcatggcgat tcccggaagg tacatcggaa gaacagattg
    10321 ataaaaccgt tgatgatttt attaacgagg ttatcgaacc gaacaaactg gcctttgacg
    10381 gcagcggtta tctggcctgg gaaggtctga tctgcatgca ggaaatcggc aaatgcaccg
    10441 aagaacatca ggcgattgtg cgtaagtggc tggaagagcg caaactggat gaggtacgca
    10501 ccagcgaact tttcgacgtt tggtgggact aagaaagcat acgggcgatg acaaatgcaa
    10561 aactgcctga tgcgctacgc ttatcaggcc tggaaagatg cacgatcgag taggcgggat
    10621 aaggtgttta cgccgcatcc ggcatggaaa acgcgtactt tgttatcaat ctggggccag
    10681 caaatgctgg cctgattttt tcttgaggga agactatgat gcgcaaaatg ctgctggcgg
    10741 cagcactttc agtgacggca atgaccgctc acgccgacta ccagtgcagc gtcacgccgc
    10801 gtgacgatgt gattgtcagc ccgcaaaccg tgcaggtgaa gggcgaaaac ggcaatctgg
    10861 tgatcacgcc agacggcaac gtgatgtata acggtaagca atattccctg aatgccgccc
    10921 agcgcgagca ggcgaaggat tatcaggctg aactacgcag cacgctgccg tggattgatg
    10981 aaggcgcgaa aagccgcgtc gaaaaagccc gtattgcgct ggataaaatt atcgttcagg
    11041 agatgggcga aagcagcaaa atgcgcagcc gtctgaccaa acttgatgcg cagctgaaag
    11101 agcagatgaa ccgcattatc gaaacgcgca gcgatggcct gacgtttcac tataaagcca
    11161 ttgatcaggt tcgcgccgaa ggccagcaat tagtgaatca ggcaatgggt ggaattttac
    11221 aggacagcat taatgaaatg ggcgcgaaag cggtgctgaa aagcggcggt aacccattac
    11281 agaatgtgct gggaagcctg ggggggctgc aatcctcaat ccaaaccgag tggaaaaagc
    11341 aggaaaaaga tttccagcag tttggcaaag atgtttgtag ccgcgttgtg actctggaag
    11401 atagccgcaa agccctggtc gggaatttaa aataatcctc tattttaaga cggcataata
    11461 cttttttatg ccgtttaatt cttcgttttg ttacctgcct ctaactttgt agatctccaa
    11521 aatatattca cgttgtaaat tgtttaacgt caaatttccc atacagagct aagggataat
    11581 gcgtagcgtt cacgtaactg gaggaatgaa atggagtttt tcaaaaagac ggcacttgcc
    11641 gcactggtta tgggttttag tggtgcagca ttggcattac ccaatatcac cattttagca
    11701 accggcggga ccattgccgg tggtggtgac tccgcaacca aatctaacta cacagcgggt
    11761 aaagttggcg tagaaaatct ggttaatgcg gtgccgcaac taaaagacat tgcgaacgtt
    11821 aaaggcgagc aggtagtgaa tatcggctcc caggacatga acgataatgt ctggctgaca
    11881 ctggcgaaaa aaattaacac cgactgcgat aaaaccgacg gcttcgtcat tacccacggt
    11941 accgacacga tggaagaaac cgcttacttc ctcgacctga cggtgaaatg cgacaaaccg
    12001 gtggtgatgg tcggcgcaat gcgcccgtcc acgtccatga gcgcagacgg tccattcaac
    12061 ctgtataacg cggtagtgac cgcagctgat aaagcctccg ctaatcgtgg cgtgctggtg
    12121 gtgatgaacg acaccgtact ggacggtcgc gatgtcacca aaaccaacac caccgacgta
    12181 gcgaccttca agtctgttaa ctacggtcct ctgggataca ttcacaacgg taagattgac
    12241 taccaacgta ccccggcacg taagcacacc agcgatacgc cattcgatgt ctctaagctg
    12301 aatgagctgc cgaaagtcgg catcgtttat aactacgcta acgcatccga tcttccggct
    12361 aaagcactgg tagatgcggg ctatgatggc atcgttagcg ctggtgtggg taatggtaac
    12421 ctgtataaat ccgtgttcga caccctggca accgccgcga aaaacggcac tgcagtagtg
    12481 cgttcttccc gcgtaccgac gggtgctacc actcaggatg ctgaagtgga tgatgcgaaa
    12541 tacggcttcg tcgcctctgg cacgctgaac ccgcaaaaag cgcgcgtcct gctgcagctg
    12601 gctctgacgc aaaccaaaga tccgcagcag atccagcaga tcttcaatca gtactaatca
    12661 cctcgccccg gtatcgtgcc ggggcttttt cacttcagac ttacgtccat tgccaatttt
    12721 cattacccta atgataatca ccggaataaa ttattccgcg tgaggttttt cgggtgaaaa
    12781 agcaatggat tgttggcacg gcactgctta tgttgatgac tggtaatgcc tgggcagatg
    12841 gcgaaccgcc aactgaaaat atcttaaaag atcaattcaa aaagcagtat cacggcattc
    12901 tcaagcttga tgctatcacc ttaaaaaatc ttgatgctaa gggtaatcag gccacctggt
    12961 cagcggaagg cgatgtctct tccagtgacg atctctatac ctgggtcggt cagttggcag
    13021 attacgaact gctcgaacag acctggacga aagataaacc ggtaaaattc tcggcgatgt
    13081 taaccagtaa aggaacgccc gcgtctggct ggtcggtgaa cttttactct tttcaggcgg
    13141 cagccagcga tcgtgggcgg gtggtcgacg atatcaaaac gaataataaa tatctgatcg
    13201 tgaatagcga agatttcaat tatcgcttta gtcagcttga atctgcgttg aatacccaga
    13261 agaactcgat tcctgcgctg gaaaaggaag tgaaagcgct tgataagcaa atggtggcgg
    13321 cgcagaaagc agcggatgca tattggggaa aagatgcaaa cggtaagcaa atgacccgcg
    13381 aagaagcctt taaaaaaatt caccaacagc gtgatgagtt taataaacag aacgatagcg
    13441 aggcgttcgc ggttaaatat gacaaagagg tttatcaacc ggcgattgcg gcatgtcata
    13501 aacagagtga agagtgttat gaagtgccga ttcagcagaa acgagatttc gatatcaacg
    13561 aacaacggcg acagactttt ctgcaatcac aaaaactcag ccgtaaattg caggatgact
    13621 gggtaacgct cgaaaaaggg caatatccgt taacgatgaa agtttcagaa ataaacagta
    13681 aaaaagtcgc gattttgatg aaaattgacg atatcaacca agctaatgaa cgctggaaaa
    13741 aagataccga acaactgcgg cgcaatgggg tgattaagta agtttgatat agtgcacgat
    13801 ttgtagcagg atgcggcgtt gaccccgcat ccggcaatca acgtctgatg cgacgcttgc
    13861 gcgtcttatc agacctacaa ccgctgccac atgtcggctg gataaggcgt tcacgccgca
    13921 tccggcaata caagtttact cagccagaaa aagctccagc agcgaattta aaaacagctt
    13981 cccatgttcc gttatctgcc agtaatccgc acattcggtg agataaccct gggcaatcgc
    14041 ctcgtctaac tgtgggcgaa tcacatcttc gcaaagcccg gtatacgcac taaactctgc
    14101 gcgtggcgcg gcctccagca agcgaaagcg gttcataaag aactcaaacg gcttatctgc
    14161 ggcttcgaca tctcgctggc tttccagata ccttccttgc ataaaaccac gcggatgacg
    14221 cgttttagtg gtacgcagaa tgcgcccatc cgggaaggtc actttgccgt gcgcgccgca
    14281 gccaataccg atgtagtcgc caaagcgcca gtagttgaga ttgtgctggc actgataact
    14341 gggtttggcg taagcggaag tttcatattg ctgataaccc gctgcggtta ataactgatg
    14401 cccctgttcg aatatatccc acagcgcgtc gtcgtccggc agcaccggtg gtcgcgaacc
    14461 aaacagcgta ttgggttcga tggtcagttg ataccaggaa agatgcggcg gattcagttc
    14521 aatggcctgg cgtagatcgc caagcgcctc ttccagtgat tgatccggta gtccatgcat
    14581 caaatcaagg ttaaagctac gtaaccctaa accgctcgcc agctttgccg ctcgtttcgc
    14641 ttcttgcggg ccatgaatgc gcccaagtcg tttcagcttt tcttcgctaa aactctgcac
    14701 accaatagag atgcggttca caccagcacg ctgataatcg acaaagcgat cggcttctac
    14761 cgtaccaggg ttcgcttcca tagtaatttc tgcatccgct gccagcggca aacgcgcacg
    14821 cacgccgtcc agcagcgttt gcatcgccgg gccggaaagc aggctcggcg taccaccgcc
    14881 aataaagatt gtctttactt cacggccctg agcgtaagcc acatcattgt ccagatcgca
    14941 aagcagatgc tgaacgtaat cgtcgtgcgg cacttctcct ttcaacgcgt gagagttgaa
    15001 atcgcagtac gggcatttct gcacgcacca cgggatgtga atgtagagac tcagcggcgg
    15061 taatttaacc attacgtaaa gcgtccaaca gcagtttcaa tgcctgacca cggtgggaaa
    15121 tggcgctctt ttcttcgcgg gtcagttcgg cagcggtttt cccttcggaa ggtacgaaga
    15181 agattggatc ataaccaaag ccaccagtgc ccgctggttc acgagtaatc acgcccggcc
    15241 agctaccgtg gcacaccagc ggagtcggat cttccgcgtg acgcagatat accagcacgc
    15301 agtggaaacg cgcctgacgt tggtcgtccg gtacgtcttt cagtgtttcc aacagttttt
    15361 gcagattctt ttgatcggtc gcgtcttcac cggaataacg cgcggagtaa atacccggcg
    15421 cgccacccag cgcatctacc gccagaccag agtcgtcggc aattgccggt aaaccggtca
    15481 cttttgccgc atggcgcgct ttcagaatcg cgttttcgat aaaggtcagg ccggtttctt
    15541 cagcggaatc tacgccgagg tctgtttggg ccacgatatc aagaccgaag tcgctaagca
    15601 gcgacgccag ctcacgcact ttaccggcat tgccggttgc gaggacaact ttttgcatag
    15661 gatacctaat taattaacgc cgcgatttct ggcgggattt gttgcggatt aatgatttta
    15721 atttgtttgt ggcggccaag ttcgcctttt tcaattacca cctggctttt ggctacccgg
    15781 aattgcttac cgagaaactt caccagatgg ctgttggcct ggccgtcaac cggcggcgcg
    15841 gtaatggcga ctttaacttc gtcgccatgt aaaccgacaa tagaatcacg gctggctttc
    15901 ggctgaatat agagccgtaa aaccagaccg tcatcattaa ctgttacggc actcataacg
    15961 ccatccacag ccccggcagc agcatgtttc cggttgcctg taatacttct gcgacaccca
    16021 tattgatgac atacagcagc agaacgagga tcatcggcga gaaatcaatc ccacccattg
    16081 ccggtagcag gcggcgaatc gggcgcagca gcggatcggc cagctgaatc agcacgtatt
    16141 caatcggact acgaccctgg cttacccagc tcataatcgc catcaccagc aggacccaga
    16201 aaatcagcaa gccgatggtt ttcagcagaa tcagtaaacc ggcaatccag atgattggca
    16261 ggaaggtcac cactttaaac agcacaatgg ctttgataaa actgagaata taggcaacca
    16321 gcagcgaggc gctgtcaatg ggacccattg ccggaataac gcggcgcagt ggcccgataa
    16381 ttggctgcgt taccttcact acaaactgtg agaaggggtt gtaaaaatca caatgagccc
    16441 actgcatcca gatgcgtaat aacagcacca tggtatacag ctcaatgacc gttgaaagca
    16501 ggaaagtcaa cgtattcatg gcgttccttt aattccttat tttttagagt aatcacgcgc
    16561 accaaaaatt gcagtgccga tacgaaccat cgtgctacct gccgcaatgg cggcttccat
    16621 atcgtccgac attcccagag agagcgtgtc gatatgcggg tagcgcgttt tcagtccggc
    16681 aaatgctaca gccatttggc gtgcaacttc aaactgcctt acatattctg actcaggcgc
    16741 agggattgcc atcagcccgc gcagacgtaa acgcggtagt tcagcgaccg ccgctgccag
    16801 ctcgtccagt tctgccagtt gaatcccgga cttactgttt tcatcactaa tattaatttg
    16861 aatcagtacg ttaagagggg gaagttctgc cgggcgctga tcgttgaggc gggtagcaat
    16921 gcgcaaacgg tcgatggtat gacaccagtc gaaatgctct gccaccaggc ggcttttatt
    16981 agactgcaac gggccaataa aatgccattc taatcctgtt acgcccagtt cctgaaagtg
    17041 gcgaatttta tctacccctt cctgaacgta gttttcacca aattgacgct gcccggcatc
    17101 aatggcttct gcgatggcgc tcgcaggttt tgttttactg actgcaagca gcgtaatttc
    17161 ttctggagaa cggccgcaac gcgttgcagc cgctgagatt ttgtcccgga cctgtgccag
    17221 gttatgcgca atatcgttca ttttccgagg atcttagtat gaatatggaa gaaattgtgg
    17281 cccttagtgt aaagcataac gtctcggatc tacacctgtg cagcgcctgg cccgcacgat
    17341 ggcgcattcg cgggctaatg gaagctgcgc cgtttgatgc gccggacgtc gaagagctac
    17401 tgcgggagtg gctggatgac gatcagcggg caatattgct ggaaaatggc cagctggatt
    17461 ttgccgtgtc gctggcggaa aaccagcggt tgcgtggcag tgcgttcgcg caacggcaag
    17521 gtatttctct agcattacgg ttgttacctt cgcactgtcc acagctcgaa cagcttggtg
    17581 cgccaccggt attgccggaa ttactcaaga gcgagaatgg catgattctg gtgacggggg
    17641 cgacggggag tggcaaatct accacgctgg cggcgatggt tggctatctt aatcaacatg
    17701 ccgatgcgca tattctgacg ctggaagatc ctgtggaata tctctatgcc agccagcgat
    17761 gtttgatcca gcagcgggaa attggtttgc actgtatgac gttcgcatcg gggttgcggg
    17821 ccgcattgcg ggaagatcct gatgtgattt tgctcggaga gctacgtgac agtgagacaa
    17881 tccgtctggc gctgacggcg gcagaaaccg ggcatctggt gctggcaacc ttacatacac
    17941 gtggtgccgc gcaggcagtt gagcgactgg tggattcatt tccggcgcag gaaaaagacc
    18001 ccgtgcgtaa tcaactggca ggtagtttac gggcagtgct gtcacaaaag ctggaagtgg
    18061 ataaacagga aggacgcgtg gcgctatttg aattgctgat taacacaccc gcggtgggga
    18121 atttgattcg tgaagggaaa acccaccagt taccgcatgt tattcaaacc gggcagcagg
    18181 tggggatgat aacgtttcag cagagttatc agcaccgggt gggggaaggg cgtttgtgac
    18241 gttgccatgc ccgatgcgca agcttatcgg gcctacaaag gcaataaatg tctgatgcga
    18301 cgctaacgcg tcttatcaga cctacagccg ctgccagatg tgtgctggat aaggtgttca
    18361 cgcagcatcc ggcaaaatgg ccggatgacg gtaacgacta tccgccaggc ttttaaatcg
    18421 ccttaatatc cctgctcgaa atagctttcg agaataataa ccgcagaggc agagtcaact
    18481 ttgcctttgt tgagcgcccg atagccgccc tgttcaaaca gaccggaacg ggcttccacg
    18541 gtgctaagac gctcgtcatg gagctttact tcaacaccga aacgaccatg aatacggttg
    18601 gcgaatttac gcgctctggc agtcaatggt tgctcggtgc cgtccatatt cagcggcaaa
    18661 ccgacgatga tttcgtccgg ctgccactct ttcagtaaac gctcgataat gttccagtcc
    18721 ggcgtaccgt cctgtgcttt aattgcaggc aaagggcgag cggtgccggt aatgcgttgg
    18781 ccgaccgcta cgccaatgct tttggtgccg aagtcgaagg cgagtaaggt tccactcatc
    18841 aggcgtgtcc tgccacacca ggcatggtga gaatatccac accaatcagt tttgccgcct
    18901 cgcgccagcg gtcggcaatc ggcgttttga acagaatatt cagatctgcc ggggccgtta
    18961 gccacgcgtt atcgagaatt tcttgttcca gttgaccttt ctcccaggag gcataaccca
    19021 gagccaccaa tacgtcagac ggttgtttat cggtgccgag cgtttccagc acatcgcggg
    19081 aagtggtcat taccgtgttg tctgaaatgc gaatgctgga agcaaaattg gagggcggag
    19141 tatgcaaaat aaacccgcga tcttcagcca gcggaccgcc gagcataacc ggtttatcca
    19201 gacggattga ttcatcacgc ggctccggcg tgatcttcag cttttccaga atcccttcaa
    19261 ttttgagatt ttccagcggc ttgttgacga tgatccccat tgcaccattg gtattatgtt
    19321 cgcaaatgta gaccacggaa cgacggaaaa tcggatcctg gagagcaggc atggcaataa
    19381 gaaagtgatg ctgtaaattc attgtcagag gttctgtttc ctggttcaaa aaagcaacag
    19441 cgcccagtat gcgtggaaag aagggcgctg tcactcagag tctcaacgag atccttctcg
    19501 ctaaggtggg ttactgctgc tgtaaacgtg cttcgatggc atccattaac attccggtga
    19561 tcgacaccgg aaactctgct tcaatctcac gaatacaggt tgggctggtg acgttaattt
    19621 cagtcagacg gtcgccgatg atatccagac caacaaaaat cagccctttt tctttcagcg
    19681 tcggcccgat ctgacgggcg attttccagt cactttccgt cagcggacga ggttcaccgc
    19741 gaccaccggc agccagattg ccacgggttt cgcccccctg cggaatacgc gccaggcagt
    19801 acggtaccgg ctcgccatcc accaccagca cgcgtttgtc gccatcttta atggctggca
    19861 ggtaattttg cgccatgcag tagcgagtgc catgctcagt cagggtttcg gcaatcacgc
    19921 cgaggtttgg atcgccttct ttcacgcgga aaatcgacgc gccgcccata ccgtccagcg
    19981 gcttaagaat gatgtcgctg tgtttctccc agaacgcttt tagctgcgct ttattgcgcg
    20041 taaccagcgt ttctggcgtt aagtcagaga accaggcggt aaacagtttc tcgttacagt
    20101 cgcgcaggct ctgcggcttg ttaacgatca gcgtcccttt ctcttcggca cgttccagaa
    20161 tataggtcgc gtagataaac tcggtatcaa acggcgggtc tttacgcatc aggatcacat
    20221 cgagatcggc cagcggcaga tcctgttcac cgacgaacga aaaccactct tcgtagttct
    20281 gcttcacgtt cagcgtgcgg gtatgggcgc gggcttcacc attgatcaga tacagatcgc
    20341 ccatctccat atagtgaagt tcgtaaccac gacgctgtgc ttccagcaac atagcaaaac
    20401 tggaatcttt cttgatgttg atgtttgcga tggggtccat cacgatgccg agcttgatca
    20461 ttattcttct ccgttagccc aaatcgccaa atcgtacttg tagcgcggta atggcggtga
    20521 gcgcagttgt ctctgtacgc aaaacgcgag gtcccaacag gatatcagta aattgatagc
    20581 gggcagtcat ggcaatttca tctgccgata aaccgccttc cgggccaatc agcaggcgga
    20641 cgcgttcaac cggtaacggc aacgtattga tgctgttact ggcgcgcggg tgaagattca
    20701 gtttcagtcc ttcatcctgc tctgcacacc aggcttccag atccatcgct ggacggattt
    20761 ccggcacccg gttacgacca cactgctcac aggcagcaat tgcaatcttc tgccactgct
    20821 gaagcttctt gttcagacgt tcactatcca gtttaacgcc gcagcgctca gaaaaaagtg
    20881 gcgtaatgag gcttacaccg agttcgatcg atttctggat agtaaattcc attttttcac
    20941 cacgcgacat cacctgaccg aggtgaatat gcagcggaga ttcgcgatcg tcgatctggc
    21001 cttccagcac cttcacttcc acgctttttt tgctggcgct ggtaatttcg gcgtcaaaga
    21061 cctggttgct accgtcaaac aattgcaacg cctgccccgg ccccatgcgc agtacgcgcc
    21121 cgatatggtt ggcggcatct tcgcaaagcg cgatgtgaga atggctggtc agtggttcag
    21181 gatgataaat gcgggggata cgcatagtta aaaatccgcg tcgtctcccc acgcggttgt
    21241 acgcgtgggg taggggttaa caaaaagaat cccgctagtg taggttagct ctttcgcgcc
    21301 tggcaagcgc gttgcacata cgggttatga ttgccctgca ccttcgcgat gcgttcatcg
    21361 cgctcgcact cccagtcggt aaccggatac atcttgttcc atgcgttgaa cagctgcgtt
    21421 tgctggcgag agagtgtcag gttgtattgg tcgcgcatat agaagtaggt gcgcgcaatg
    21481 gcaccgcgtg cacgcgctgg tggttcggca gctttttctt tgaaatcgac cttcatggcg
    21541 cattgaccgt actggccttc accgccattc cactggctgt acataaagtt gccgcgatcg
    21601 ccattcacct caccgactga cggctgcagg ttatgcatat cgctttccat cttgcgatag
    21661 accggatctt tagcgcagtt tttacgtcca ccgtcctgcc agcactggcg ctggtgaccg
    21721 aactgccagg cgggaacgac atgttcccac tctacgcggc tggcgcggtt ttcatttttg
    21781 cgcacctgat agccgcacga ttgcagatca acaacgcctt ttttgccctg ccagttaatt
    21841 ttacatccgc aataaaacgt accgggcgcg tcagcgtgga cttttaccgc cgcggctttc
    21901 gcctgagaaa aactattgat accttcggcc aacgccgggc cggaaaatgc tgcgctcagt
    21961 accaccgcag caatagacaa ataacggtac atcacttact ccgtgttaaa acgagccagc
    22021 aacgtagcga aagttgtttt ggtatgcaat cagccagatc aggaaagttc ctgatagttc
    22081 agatggttat ttcgcaacca gctgttcacc gcagtgaaca cagcgataga cggcctcgcc
    22141 acgaactacg cgattatggc ggcgtacggt aagctgatgc tcctggcact tgcagcggta
    22201 ggggaaggtg ttgcgacgca cggattgcag ttcgaactga tgcgtacgac gggcgggaac
    22261 acccagcacg ttttccatca tccacttcca ctctttgcca tgtggcgcta cgcggccgaa
    22321 atgtttccat accagcaaat gtgccagttc gtgcggcacc acttcttcaa taaaggcttc
    22381 actgttttcc aacagcaaaa cgggattgag gcgaatttca tagctttcca gccaggctgt
    22441 tccggcggag gttccgcgct gggtgtaaga gagttttggc tccgggtagt tacgccctag
    22501 cttcaggttg gcctgggcga gtttttcccg caggcgacgc ataacggcct gttggatggc
    22561 gatagggaga cgggatgttt tcatagcggc agaggataga gcgaagaaaa gatgactgca
    22621 agaggtggct tcctccgcga tgggaggaag cttggggaga ttaatcgtga gcgcctattt
    22681 cgcgcagttt acgacctttc atcagattac gttcaatatg ttccagcgaa acgtgtttgg
    22741 tttccggtac cagccacaat gtcagcagga taaacagtac gttcagagcc gcatacaccc
    22801 agaaggtgtt ggcgttaccc agcgtgttga gcatggtcag gaacgttgcg ccaacgatca
    22861 tgttggcaat ccagttggtg gcagtggagc aggtgatgcc aaaatcgcgg cctttcagcg
    22921 gctgaatttc ggagcacagt acccaaatca gcggaccggc actcatggca aaaccgacaa
    22981 taaacatcag cagcatggcg atggcgaaat actgcgccga cggagagtga ataccgatat
    23041 gcatcattgt accgagtacg cccatgccag cagccatcac caggaagccc agcgttagcg
    23101 ttggtttacg tccccagcgg tcaacaaggc cgattgcgat aaaggtggca agtacgttgg
    23161 tcaggccgac aatcacggtc ccccacattt gctcggtagt gttggtataa cccgccagtt
    23221 cgaagatttt cggcgcgtaa tacatgatga cgttcatccc ggtgaattgc tgcattacct
    23281 gcaacagtac gccaaggaac accgcgcggc ggaagttgct gttctcttta aacagcgccc
    23341 agccactctg tttaacctgc aaactttcac ggatttcatc cagttcgcgt ttcgcttccg
    23401 cgctggtgtc acgcaggcgt agcagcacgc gttcggcatc aacaaaacgg cgtttggcgg
    23461 caaaccaacg tgggctgtct ggcaggaaga agacaccaat cagcagcaaa attgccggga
    23521 tgataatcac acccagcatc cagcgccatg caccggtgta gctgaaggct gtatcagaaa
    23581 gataagcacc gaggatcccg atagtgatca tcaactgata catcgagatc atactgccac
    23641 gaattttttc cggcgcaatt tcagagaggt acagcggtgc ggtataagag gccacaccca
    23701 ccgccagccc cagtagaacg cgggaaagaa tcagtacttc aacgtttggc gcagccgcag
    23761 agaacagcga accggcaaca aacaaaattg cgccgatcat caggctcttt ttgcgcccga
    23821 gtttaaagga gagccagccg ctgcccaccg caccgactgc cgcaccgaac atcatggagc
    23881 ttacgaccca ttcttgcgtg tgcgaagtaa tctggaattc atctgcaata aacggcagtg
    23941 cgccagcaat tacaccgata tccaggccaa agagtaatcc cgccagagcg gcaaggaagc
    24001 agacgaaaaa cgtcattgcc ttgtttgacc gcccctgttt tttagcgtca ggcatgatgc
    24061 cctccaatat ggttattttt tattgtgaat taagataggt gagtacgacg taaaaagatg
//
LOCUS       DAPHBJ010000056        23837 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.056, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000056 DAPHBJ010000000
VERSION     DAPHBJ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.056"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            12..530
                     /locus_tag="RHZ76_001979"
     CDS             12..530
                     /locus_tag="RHZ76_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDV2812625.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQMGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            546..1085
                     /locus_tag="RHZ76_001980"
     CDS             546..1085
                     /locus_tag="RHZ76_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HDV2812626.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1178..2755)
                     /gene="guaA"
                     /locus_tag="RHZ76_001981"
     CDS             complement(1178..2755)
                     /gene="guaA"
                     /locus_tag="RHZ76_001981"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDV2812627.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2824..4290)
                     /gene="guaB"
                     /locus_tag="RHZ76_001982"
     CDS             complement(2824..4290)
                     /gene="guaB"
                     /locus_tag="RHZ76_001982"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDV2812628.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4452..5822
                     /gene="xseA"
                     /locus_tag="RHZ76_001983"
     CDS             4452..5822
                     /gene="xseA"
                     /locus_tag="RHZ76_001983"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDV2812629.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLKKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSITTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5819..6034)
                     /locus_tag="RHZ76_001984"
     CDS             complement(5819..6034)
                     /locus_tag="RHZ76_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDV2812630.1"
                     /translation="MELHCPQCQHVLDQDNGHAHCPSCGEVIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6103..7575)
                     /gene="der"
                     /locus_tag="RHZ76_001985"
     CDS             complement(6103..7575)
                     /gene="der"
                     /locus_tag="RHZ76_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDV2812631.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7693..8871)
                     /gene="bamB"
                     /locus_tag="RHZ76_001986"
     CDS             complement(7693..8871)
                     /gene="bamB"
                     /locus_tag="RHZ76_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HDV2812632.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8882..9502)
                     /gene="yfgM"
                     /locus_tag="RHZ76_001987"
     CDS             complement(8882..9502)
                     /gene="yfgM"
                     /locus_tag="RHZ76_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HDV2812633.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9520..10794)
                     /gene="hisS"
                     /locus_tag="RHZ76_001988"
     CDS             complement(9520..10794)
                     /gene="hisS"
                     /locus_tag="RHZ76_001988"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDV2812634.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNMLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10905..12023)
                     /gene="ispG"
                     /locus_tag="RHZ76_001989"
                     /gene_synonym="gcpE"
     CDS             complement(10905..12023)
                     /gene="ispG"
                     /locus_tag="RHZ76_001989"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDV2812635.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12050..13063)
                     /gene="rodZ"
                     /locus_tag="RHZ76_001990"
     CDS             complement(12050..13063)
                     /gene="rodZ"
                     /locus_tag="RHZ76_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HDV2812636.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13348..14502)
                     /gene="trmG/rlmN"
                     /locus_tag="RHZ76_001991"
     CDS             complement(13348..14502)
                     /gene="trmG/rlmN"
                     /locus_tag="RHZ76_001991"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HDV2812637.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14652..15083)
                     /gene="ndk"
                     /locus_tag="RHZ76_001992"
     CDS             complement(14652..15083)
                     /gene="ndk"
                     /locus_tag="RHZ76_001992"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDV2812638.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15232..17544)
                     /gene="pbpC"
                     /locus_tag="RHZ76_001993"
     CDS             complement(15232..17544)
                     /gene="pbpC"
                     /locus_tag="RHZ76_001993"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HDV2812639.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKITTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NSIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTSCP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17545..22506)
                     /gene="yfhM"
                     /locus_tag="RHZ76_001994"
     CDS             complement(17545..22506)
                     /gene="yfhM"
                     /locus_tag="RHZ76_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HDV2812640.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHLTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSSLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEQLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22713..23558
                     /gene="sseA"
                     /locus_tag="RHZ76_001995"
     CDS             22713..23558
                     /gene="sseA"
                     /locus_tag="RHZ76_001995"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HDV2812641.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKYLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
BASE COUNT         5708 a         6606 c         5931 g         5592 t
ORIGIN      
        1 aacaaggaat agtgatgaaa tttaaaaaat gtcttctgcc tgtggcaatg ttagcgtcat
       61 tcactctggc aggatgccag tcaaatgctg acgatcatgc tgccgatgtt tatcaaaccg
      121 atcaactgaa taccaaacaa gaaactaaaa ccgttaatat tatttccatt cttcccgcaa
      181 aagttgccgt agacaactcc caaaataaac ggaacgcaca agccttcggc gcgcttattg
      241 gcgcagtcgc tggcggtgtt atcggccaca acgtcgggtc tggcagcaat tccggaacga
      301 cggcaggtgc agttggcggc ggagctgtag gcgcggcagc gggttctatg gtgaatgata
      361 aaaccttagt ggaaggtgtt tctttaacct ataaggaagg caccaaagtg tatacctcta
      421 cccagatggg taaagagtgc cagtttacga caggtttagc cgttgttatt accacgacgt
      481 ataacgaaac gcgtattcag ccaaatacca aatgtcctga aaagagctaa taatcaggag
      541 gagtcatgaa gaaagttttt ctttgcgcca tcttagcctc cttaagctat ccggctatcg
      601 cctcatcatt gcaggatcaa ctctctgctg tcgcagaagc ggaacagcaa ggtaaaaatg
      661 aagagcaaag gcagcatgac gaatgggtcg cggagcgcaa cagggaaatc cagcaagaga
      721 agcaacgtcg cgcaaatgcc caggccgccg ctaacaaaag agcggcaacg gcagcggcaa
      781 ataagaaagc tcgtcaggat aaactggacg ccgaagcctc tgcggacaaa aaacgcgatc
      841 aaagttatga agatgagcta cgcagcttag agattcagaa acaaaaactg gcgctggcga
      901 aagaagaagc ccgcgttaag cgagaaaacg aatttatcga tcaggaactg aagcacaaag
      961 ctgcgcaaac cgatgtggtg caatctgaag ctgacgccaa cagaaatatg actgaaggcg
     1021 gtcgcgatct gatgaaaagc gtgggcaaag cagaagagaa caaatcggac agctggttta
     1081 attaatcgat gttagtaact tcaatcctat aattcttgaa gataaaaaac cctctgtagt
     1141 aacagagggt tttgttcatt catagtgcag ggtcaaatca ttcccactca atggtagctg
     1201 gcggcttgcc gctgatgtca tacaccacgc gggaaatacc gttcacttca ttgataatgc
     1261 ggttggaaac gcgaccgagg aaatcgtacg gcagatgcgc ccagtgtgcg gtcataaagt
     1321 cgatggtttc gacagcacgc agagagacaa cccagtcata cttacgacca tcgcccatta
     1381 cgccaacgga acgtaccggc aggaacacag tgaacgcctg gctgactttg tcgtacaggt
     1441 ccgctttacg cagttcttca atgaagatgg cgtcagcacg gcgcagcagg tcacagtact
     1501 ctttcttcac ttcacccagc acacgaacgc caaggcctgg tcccgggaat gggtgacggt
     1561 acaacatgtc gtacggcagg cccagctcca gaccaatctt acgcacttcg tctttgaaca
     1621 gctctttcag cggttcaacc aggcccatct tcatctcttt cggcaggccg cccacgttgt
     1681 ggtgagattt gatgacgtgt gctttaccgg ttgcagacgc cgcagattcg ataacgtcag
     1741 ggtagatggt gccctgcgcc agccacttca cgtcttccag tttcagcgct tcttcatcga
     1801 atacttcaac gaaaacgcga ccgatgattt tacgttttgc ttccggatcg ttttcgccag
     1861 ccagcgctga caggaagcga tcttctgccg gaacgtgaac aatgttcaga ccaaagtgat
     1921 cgccaaacat atccagaacc tgctctgctt cgttgagacg cagcaggccg ttgtcgacga
     1981 atacgcaagt caggttttta ccgatagcgc ggtgcagtag cattgcggtt acggaggaat
     2041 ccacgccacc agagaggccg aggatgactt tatcgtcgcc tacctgctca cggatgcggg
     2101 ctacagcatc gtcgataatt ttcgctggcg tccacagggc ttcacactgg cagatatcac
     2161 gcacaaaacg ctccagcatg cgcataccct ggcgggtgtg agtcacttcc gggtggaact
     2221 gtacgccata gaagcgtttt tcttcgttag ccataatggc aaacgggcag ctttcggtgc
     2281 tggctacggt gacgaagtcg gacgggatag cggtaacttt atcgccgtgg ctcatccaga
     2341 catcgagcag cggtttaccg tctgcggtca gcgcatcttc gataccgcga accagtgcgc
     2401 tgtcgtttac gacttcaacc tgcgcgtagc caaattcacg ttcgttagag gcttcaacgt
     2461 gaccgcccaa ctgcatcgcc atggtctgca taccatagca aacgccgaat accggtacgc
     2521 ctgcttcaaa gacatactgc ggcgcacgcg gactgttttc ttcagtagta ctttccgggc
     2581 cgccggaaag aataatgccg cttggattga agtcacgaat ttgtgcttct gtcacatccc
     2641 acgcccacag ttcgcagtaa acgcccagct cacgcacgcg gcgcgcaacc agttgagtat
     2701 actgggaacc gaagtccaga atgaggatgc gatgcttatg aatgttttcc gtcattgacg
     2761 cttattccga ggcaagtgaa acagataata taaatcgccc gacatgaagt cgggcgaaga
     2821 gaatcaggag cccagacggt agttcgggga ctctttagta atggtcacgt cgtgaacgtg
     2881 gctttcctga atgcccgcac cgctgatacg tacaaactcc gctttagtac gcagttcgtc
     2941 gatagtacca cagccggtca ggcccataca ggagcgcagg ccacccatct gctggtgaat
     3001 gatctctttc aggcgacctt tataagctac gcgaccttcg ataccttccg gcaccagttt
     3061 gtcggcagcg ttatcgctct ggaaataacg gtcagaggaa cctttggaca tcgcgcccag
     3121 ggaacccata ccacggtaag atttgtaaga acggccctgg tagagttcga tttcacccgg
     3181 agattcttca gtacccgcca gcatggaacc caccattacc gcgcttgcgc cagcggcgat
     3241 agctttggcg atgtcgccgg agaagcgaat accaccatct gcgataaccg gaatacctgt
     3301 gccttccagc gcttcaactg cgtcagcaac ggcagtgatc tgcggaacac ctacgccagt
     3361 aacgatacga gtagtacaga tagaaccagg gccgataccg actttaaccg cgctgcaacc
     3421 agcttctgcc aaagcgcgtg cacctgcagc tgttgccacg ttgccgccga tgatttgcag
     3481 atccggatat ttagcacggg tttcacggat acgctgcaaa acgccttctg agtgaccgtg
     3541 ggaggagtcg atcagcagaa cgtcaacgcc agcggcaacc agcgcgtcaa cacgctcttc
     3601 gttacccgca cctgcgccaa ctgcagcacc aacacgcaga cggccttgct cgtctttaca
     3661 ggcgttcggt ttacgttccg ctttctggaa atctttcacg gtgatcatgc cgatcaggtg
     3721 gaattcgtca tcaaccacca gcgctttttc aacgcgtttt tcgtgcattt ttgccagcac
     3781 cacttcacgg gcttcacctt cacgcacggt gaccagacgc tctttcggcg tcatgtaaac
     3841 gctaaccggc tggttcaggt cggtaacaaa acgcacgtca cgaccggtga taatacccac
     3901 cagttcgttt tcttcggtaa cgaccggata gcccgcaaaa ccgttacgct cggtcagttc
     3961 tttcacttcg cgcagcgtcg tggttggcag aacagtctgc ggatcagtca ccacaccaga
     4021 ttcgtgtttt ttcacacggc gaacttcttc tgcctggcgt tcaatggaca tgtttttgtg
     4081 gataaagccg ataccgcctt cctgagccag agcaatagcc aggcgcgctt ccgttacggt
     4141 atccattgct gcggaaagca tagggatatt cagacgaata gttttcgtca gctgggtgct
     4201 gaggtcagca gtattcggca gaacggtaga atgagcagga acgaggagaa cgtcgtcaaa
     4261 cgtcagagct tctttagcga tacgtagcat gggcaatatc tcgaccagag tggttaataa
     4321 atattgccgc ggcattatac agagcgtaac cgattgcatc tacccctttt tgcaaaaaat
     4381 gcttgctatc cccggagggc gggttactat cgactgaata acctgctgat ttagaatttg
     4441 atctcgctca catgttacct tctcaatccc ctgcaatttt taccgttagt cgcctgaatc
     4501 aaacggttcg tctgctgctt gagcatgaga tgggacaggt ttggatcagc ggcgaaatct
     4561 ctaatttcac acaaccggct tccggtcact ggtactttac actcaaagac gacaccgccc
     4621 aggtacgctg cgcgatgttc cgcaacagca accgccgggt gaccttccgc ccacagcatg
     4681 ggcaacaagt tttagttcgc gccaatatta cgctctacga gccgcgcggc gactaccaga
     4741 taatcgttga gagtatgcag ccggccggtg aagggctgct gcaacagaag tacgaacagc
     4801 tcaaagcgaa gttgcaggct gaaggtttgt tcgatcagca atacaaaaaa ccacttccct
     4861 cccctgcgca ttgcgttggt gtgatcacct caaaaaccgg tgctgcgcta catgatattt
     4921 tgcatgtgtt aaaacgtcgc gatccttctc taccggtgat catctacccc accgccgttc
     4981 agggcgatga cgcgccgggg caaattgttc gcgccattga actggcgaat cagcgcaatg
     5041 agtgcgacgt gttgatcgtt gggcgcggcg gcggttcgct ggaagattta tggagtttta
     5101 acgacgaacg cgtagcgcgg gctatttttg ccagccgcat tccggtcgtc agcgccgtcg
     5161 ggcatgagac ggatgtgacc attgccgatt ttgttgccga tctgcgtgcg ccaacgccgt
     5221 ctgccgccgc tgaagtagta agccgtaatc agcaagagtt actgcgccag gtgcaatcgg
     5281 cccgtcaacg gctggagatg gcgatggatt attatctcgc caaccgtacg cgtcgcttta
     5341 cgcagattca tcatcgcttg cagcagcagc atccgcagct ccggctggca cgccagcaaa
     5401 ccatgcttga acgcctgaaa aaacggatga gctttgcgct ggaaaatcag cttaagcgtg
     5461 ccgggcaaca gcagcagcga ttaacacagc ggctgaatca gcaaaatcca cagccgaaga
     5521 ttcatcgcgc gcaaacgcgc attcagcaac tggaatatcg tttagcagaa atcctgcgcg
     5581 cacagcttag cgccacgcgt gaacgtttcg gtaatgcagt aacgcacctc gaagccgtaa
     5641 gcccactgtc aacgctggcg cgtggataca gcattactac tgctactgac ggcaatgtac
     5701 tgaaaaaagt gaagcaagtt aaagcgggtg aaatgctaac cacacgtctg gaagacggct
     5761 ggatagaaag tgaagtaaaa aacatccagc cggtaaaaaa atcgcgtaaa aaggttcatt
     5821 aagccagcac gaattcaacg cgttttttcg atatcaagcc atgcccgtgc tgacagaagt
     5881 aatctactgc accgcaggct tttaacacct gcaacggttg atggcagtct ggacaaagag
     5941 ctttcatttc aataacttcc ccgcaggatg ggcaatgcgc atggccgtta tcctgatcaa
     6001 gcacatgctg acattgtgga caatgcagtt ccatatgaat tcctccggta gccacccctc
     6061 tacattcata gagggaatgg cagataaaat acttacggat aattatttat ttttcttgat
     6121 gtgcttcatc agacgcttac gtttacgcat ctgggttggc gtcagggtgt tacgcttgtt
     6181 cgcatacggg ttttcccctt ctttgaactg aatacgaatc ggcgatccca ttacgtccag
     6241 cgatttgcgg aagtagttca tcaagtaacg cttgtaggaa tcaggcaggt ctttcacctg
     6301 attaccgtga atcaccacaa tcggcgggtt ataaccaccg gcgtgggcat atttcagctt
     6361 cacacgacga ccgcgtacaa gcggcggttg gtgatcttca acagccatcg tcatgatgcg
     6421 cgtcagcata gaggtcccca cacgacgggt ggagctgtca tacgcttcac gtactgattc
     6481 aaacaagtta ccaacaccac tgccgtgcaa ggcagagata aagtgcacac gagcaaaatc
     6541 gataaagccc agacggaagt ccagcgtttc tttcacctgc tctttcactt cctgactcag
     6601 gccatcccac ttattcacca caatgacaag tgagcgccca ctattgagaa taaagcccag
     6661 cagcgagaga tcctgatcgg aaataccttc gcgcgcatca atcactaaca tcaccacgtt
     6721 ggcatcttca atggcctgca acgttttgat tacggagaat ttctctacag catcggtgat
     6781 tttaccgcgt ttacgtacgc cagcggtgtc aatgagcaca tactcacgtc catcgcgttc
     6841 cattggaatg tagatactgt cacgcgtcgt accaggcatg tcataaacaa caacgcgctc
     6901 ttcaccaaga atacggttag tgagtgtaga cttacctacg ttcggacgac ccacaatcgc
     6961 cagtttgatc ggcagacttt gcgggtcgaa gtcgtcttcc tcttcttctt cgccgttctc
     7021 ttccgcttca aattgcgccc agtattcagc gtcttcgtcg acttcctctt gcggtgcgag
     7081 atcttccatc cacggcagca gcacatgctc cagcagactt aatacgccac gaccgtgaga
     7141 cgcggcgatc gggtagattt cacctaaacc aagcgagtag aaatcaacca cggcctgatc
     7201 gggatccaga ccgtcagttt tgtttgccac caggaaggtc ggtttttcac gggagcgcag
     7261 atgtttggca atcgcttcat ctgccggcat caggcccgcg cgcgcatcca ccataaacag
     7321 tacgacgtcc gcttcttcaa tcgccagcag cgactgttcc gccatgcggg tttctacacc
     7381 gtcttctgtg ccatcaatcc cgccggtatc aatacagata aactcacggc cttcaatttc
     7441 cgcacgaccg tacttacggt cacgagtcag acccgggaaa tccgcaacca gcgcatctcg
     7501 ggtgcgagtt agacggttaa ataacgtgga ttttcctacg ttagggcgcc cgacaagcgc
     7561 gaccacaggt accatgttta aagcctcatt tttataaatc atcagacaac gcacgctata
     7621 ttcgcgtcgt tgttaaaaac aggaaaacgg cccctgtcca ggagccgttt tcaaagtgaa
     7681 cgacagagac gattaacgtg taatagagta cacggttccg tcttttgcct ggatcagcag
     7741 tttgccgtca gcggcaaccg gttcagtctg gaaaccggaa ctatcaactt tttgctgggc
     7801 aacgaaacga ccatcttcga cgttaatcca gtgcagataa ccttcgctgt cacctaccac
     7861 caggttgcca ttatacagca ccggagaagt cagcaggcga tgcaacagat cgctttgtgt
     7921 ccacagcgta acgccgccat caatggtcaa cgccatcacc cggtcatttt gatcgaccag
     7981 atagatgcga ttgccgtcga cgatgaaatc attcaccgaa cccagttcgc gtttccacat
     8041 aatctgacca ctgcgcagat caagcgccgt caggttacca ttataggcca gcgcgaaaac
     8101 aacgccgtta acaacgacgg gagtcgtgtc aacatcgctc agacggtcaa tttcggtaga
     8161 accggtcgcc tgggaaatac gctgctgcca aatcatctgg ccctgttcca tcagcactgc
     8221 gctgacgcga ccattatcgc cacccacgac ggccgcacca aaagccgttg ccggcgcaga
     8281 ctcgccacgc aaagagagcg aaggcatatc gaggttaact gtccatttga cagcgccgtc
     8341 agcttcgttc agcgcttgta actgaccgtt actggtgtgg attaacacca gaccgtcgct
     8401 gaccaccggg cgagaaagtg cttcacccgc gactttagtt tgccatgcca cagtaccatc
     8461 gctggtattc agcgcgtaaa cctgcgcctt ttcgctgcca atgtagacat gcccaccaga
     8521 cacggtcaca ccgccagaaa gtaatgcagg ctctttagag aaccagccat ctttctcggc
     8581 caggctgaca gaccagattt ctttgccatc atccgcattc agcgctttta ctaaaccagc
     8641 gcggtccgct gcatagacaa cgttgtccgc cagtgccgga tgaagattgg aatagaagtt
     8701 gccaatgccg ctaccaacgg aagtgctcca cgccgtggtc ggcgtaaact ggttttcaac
     8761 ggttggcaat ggggacatct ttaccacatc ttcttcgctg ttaaacagcg aacagccgct
     8821 taaaagggta acggaaagca gtcctggcag cagtaattta cgcaattgca tcgggtccct
     8881 ctcagatgga caaattatta attttcatct gcatcatttc gctcagtgcc ggagtaacat
     8941 cgcttttcac gcctgcttcc catgcactac gcgcaccttg cttatcacct ttgctcagca
     9001 atgcttcacc acgcaggtcg gcaacaatgg cagcccaccc ttcacctttg atggtatcaa
     9061 gggttttcag cgcggcatca gcctgcttga gctgtacctg aacgcgagca agacgcagat
     9121 ttatcacggc tttgagattt tcatcgctcg tgtctgccag cccctgttgt aactgggcgg
     9181 cagctttctc tagttcattt ttgtcaacaa attgctgcgc aagttccaaa gaagccagcg
     9241 caccataagt atttttattt tcagcagcaa atttttccgc cgccgggatg ctatccggtt
     9301 tgccttcgct cactgcggta accgcatttt gataggcaag agaagcggag cgtgcagaat
     9361 caacctgatg gctgttccag tagcgccagc caatcagtgc gccaacgccc aaaatcaccc
     9421 caacagccag tgctttgcca ttttcagcaa aaaagcgttt aaccgcttct acctggtcgt
     9481 tttcgttctc gtaaatttcc acgctgtcct tctccttcct taacccagta acgtgcgcaa
     9541 atgcgcggct acgctatcct gcgcaactgc cgtttgctca ccagagcgca aatccttcac
     9601 tactgctgtg ccgttagcca cttcagactc acccagcacc acagcaacgc gggcacccca
     9661 tttatcagca cgggcaaact gtttcttaaa gttgccgccg ccgtggttgg tcatcaattt
     9721 cacgcccggt aattcatcac gcagacgctc agctaatgcc atagccgcag attgtgtatc
     9781 agcacctgaa gccaccaggt atatatcgac aacaggatcg gctttaaatt ccggattaac
     9841 ggcctgtact aacaatacaa gacgttcgag gcccatcgca aaaccgacag ccggtgttgc
     9901 acgaccgccc agttgttcca caagaccgtc ataacgaccg cctgcacaca cggtgccctg
     9961 ggagccgaga ctgttagtca cccactcgaa aacggtacgg ttgtagtaat ccagaccacg
    10021 caccagacgc tggtttacgg tgtaagcgat ccccgcgctc tcaagcagtt tgcacagacc
    10081 agcaaagtgc tcacgagatt cctcgtccag ataatcacct aatgccggag cgtcgttgag
    10141 aagcgcctgc acttccggat tttttgaatc cagcacgcgc agcgggttag tgtacatgcg
    10201 gcgtttgcag tcttcgtcca gcttttcttt atgctgctca aggaatgcca ccagcgcatc
    10261 gcggtaattg gcgcgtgctt ccagcgaacc gatagaattc agctcaagag ttacgtgttc
    10321 ggaaataccc agcgcgcgcc accagcgggc ggtgagcata atcagttcag cgtcgatatc
    10381 cggaccttgc agaccgaaaa cttcgcagcc caactgatgg aactgacgat aacgcccttt
    10441 ctgcggacgc tcgtgacgga acatcggccc gatataccac agacgctgtt cctgattgta
    10501 cagaagacca tgctcgatgc cggcgcgtac acagcccgcc gtcccttcag ggcgcagagt
    10561 caggctgtcg ccattgcgat cctcaaaggt gtacatctct ttttcaacca cgtcggtgac
    10621 ttcaccaatc gcacgtttga atagcggggt ctgctctaca atcggcaagc ggatttcact
    10681 gtaaccgtag ctgccgagca tgtttttcag tgtgccttca atgcgctgcc agatggccgt
    10741 ttcgccaggc aggtaatcgt tcatgccgcg aatggcttga atgttttttg ccacgtttat
    10801 tctctttctg aatataaaaa tgaaccctca acgcttccct caatgtttgg ggagccatgc
    10861 gggttcaatc atacacggga agcgaggcgc ttcccatcac gttattattt ttcgacctgc
    10921 tgaacgtcaa ttcgacgcgc ttcgtccagc tgactggctt tcgcacgaat gcgtgcttcc
    10981 agctggtcga tcatatcgtt gttgtccaga cggtctttgc gcacgccatc ttcatagagg
    11041 ccgcttttct tgttgccgcc ggtgacgccg agtgtagaaa ccagcgcctc acctgggcca
    11101 ttcaccacgc agccgataat cgaaacgtcc atcggagtga tgatatcttc caggcgttgc
    11161 tccagcgcgt taaccgtacc gataacatca aactcctgac gcgaacaggt cggacaggcg
    11221 atgaagttga tacctcgcga acggatacgc agcgatttca aaatatcgaa accgactttg
    11281 atctcttcga ccggatcggc cgccagtgat acgcgcagcg tgtcgccgat gccttcagac
    11341 agcagcagac ctaaaccaat ggcggatttt actgccccgc tgcgcgcgcc acccgcttcg
    11401 gtgatcccca gatgcagcgg ctgatcgatt tgttttgcca gcaaacgata agactcaaca
    11461 gcgaggaaga cgtcagacgc tttcacgctg actttgaact gatcgaagtt caggcgatcg
    11521 agatgatcaa catggcgcat ggcggattcc agcaacgcct gcggcgtcgg ttcgccatac
    11581 ttttcttgca gatctttttc cagcgatccg gcgttaacgc caatgcggat cggaatgttt
    11641 ttatcgcgcg cacagtcaac taccatgcga atacgctctt cattaccgat attgccaggg
    11701 ttaatacgca gacaatcgac gccgtattcc gctactttca gcgcaatgcg atagtcgaag
    11761 tggatgtcag ccaccagcgg cacgttaacc cgctgtttga tgagtttgaa cgcttctgcc
    11821 gcgtccatcg tcggtacgga gacacggacg atatcagcgc caacgcgttc cagcgccttg
    11881 atttgattga ccgttgcttc gacatctgtc gtacgcgtat tggtcatgga ctgtacggcg
    11941 atgggagcac catcgccaat cggcacattc ccaacgtaaa tacgtgttga ttttctacgt
    12001 tgaattggag cctggttatg catgaaaaat ctcccgcgtt acccgtctgt tactgcgccg
    12061 gtgattgttc ggcattgagg gtcagacgcg caacctggtt agttctgata aaacgactca
    12121 gatcgacagg tttcccttga tactggatct gtactgcggc tggcgcacca attttcagtt
    12181 tgtacggtgc ctggccggtt aagttcaaat taccgtcttt acgctgcata ccgctaaaca
    12241 attttttacc ggtagcatca gtgacctcca gccagcaatc ggcagtaaag ttcatcacca
    12301 gcgcattcgg atcagccacc ggcgtggtca cgccagcctg atcggttggc aacggcgcag
    12361 caccatctgg cgttgttgct gccgttggtg ccggggtcgc ggcggtatca acatttgcct
    12421 gcgaaggcga aacaaccgca ttctgttgcg gatcaacagc tggtgctggc gcagttacgg
    12481 caggtgtttg cgtgttggtt gcggtagtat ccacagacgc tggcggcgtg gacgttgtag
    12541 ccgggtctgt agttgtcgac gtatttaacg gaacactctg cccctgctcg ctattactgc
    12601 tcagttccgc cgaagattga tcggccatag tggtgatctc ttcctgctga gctttgtggt
    12661 cttgccacca ccaggcaccg ctcaggccga taaccacaaa caacaccagc caggtgaagg
    12721 tcatcagcca accatcacgt tttttgcggc gtttaccgag ggaaaaactc tgcattggcg
    12781 caacttttgc agcccgtagt ggagcctgct tttccagccc tggcagcagt tcttcttctg
    12841 gaatatgtac cagacgcgca taagagcgga tatatccgcg caggaatgtt gaagcaagat
    12901 cggcgggtgc cttatcttct tcaatgtcgc gtaccgtgga aaccttcagg caaagtcgct
    12961 cggcaacggc ctgctgacta agtcctagtt gttcgcgagc attacgcagg cgagcgccgg
    13021 tagtaagtgc ttcattttgg tcgtgcgtgg cttcagtatt cattcgctgc aggtacgttt
    13081 aaatgagaat tgagatgccg gtgaaccatc atgctcaccc acaccgcgaa acatccgtta
    13141 agttaaccgt tatcatacag tataagaccg tctgtgtgct cgtgacaaag cccacattca
    13201 ctaaggctaa cttactgttg catcgttaca tactgcctta aagtcagcaa aaacgcaccg
    13261 ttaatattga ccagacaatt gcaacttaat acttcattca taaaacatta cgccacggta
    13321 cataaagtaa ccgtggcgta atggctatca gaccgcttta atgtcgatgg cttcaccctg
    13381 catccgttta cgcagggtac gtttcgtacg gtcgataaca tcgcccgcca actgaccaca
    13441 ggcagcatcg atatcatcac cacgcgtttt acgcacaata gtggtgaaac cgtagctcat
    13501 cagcactttt gagaaacggt cgatacggct gttcgagctg cgtccatacg gcgcacccgg
    13561 gaacgggttc cacgggatca ggttgatctt acacggcgta tctttcagca gttccgccag
    13621 ttggtgagcg tgttcagtgc cgtcgttaac gtggtcaagc atcacgtatt caatagtgac
    13681 tcggccttga ttggcgttgg atttctccag ataacggcgc accgcagcaa ggaacgtttc
    13741 gatattgtac tttttgttga tcggcacaat ttcgtcacga atttcgtcgt tcggcgcgtg
    13801 cagggaaatt gccagtgcaa cgtcgatcat atcgcccagt ttatccagcg ccggaactac
    13861 accggaagta gaaagcgtga cgcgacgttt agacaggcca aaaccgaaat catcaagcat
    13921 gatttccatc gccggaacga cgttgttcag gttgagcagc ggctcgccca tgcccatcat
    13981 cactacgtta gtgattgggc gctgaccggt gacttttgct gcgccgacga ttttcgccgc
    14041 acgccacacc tggccaataa tttccgatac ccgcaggttg cggttaaagc cctgctgggc
    14101 ggtggaacag aatttacact ccagcgcaca ccccacctgc gaagagacgc agagcgtggc
    14161 acggtcgtct tccgggatat acaccgtttc gacgcgctga tcgccaacgg cgatcgccca
    14221 tttaatggtg ccgtcggatg aacgctgttc ttcaaccact tccggtgcgc ggatttccgc
    14281 cacctctttc agtttgccgc gcaacacttt gttgatgtcg gtcatctcat caaagttgtc
    14341 gcagcaatag tgatacatcc acttcatcac ctgatcggcg cggaagggtt tttcacctaa
    14401 atctttaaaa aactcccgca tctgctgacg gttgagatcc agcaggttga tttttccatc
    14461 tttcgtggtg acgttttcag gtgtgactaa ttgttcagac atatgctatt ccggcctcgt
    14521 tattacacgt tatggcccct ggagggttga aaaaagaaac gccccggtga gcggctgctc
    14581 gtccgggggc gctgcattgt acaaattctg gcgcacggat gccacgtttg cacgcggcat
    14641 ttacgaaatt attaacgggt gcgcgggcac acttcgcctt cgccaaagaa ataagcgatt
    14701 tcgcgagcgg cagattcgac ggaatcagaa ccgtgggtac cgttttcggt caggctgtca
    14761 gcgtaatcag cgcgcagagt accagccagt gcgtttgccg gattggtcgc gcccagcaga
    14821 tcgcggtgac gctgaacggc gttttcacct tccagcacgg aaaccacgat cgggccagag
    14881 gtcatgaatt caaccagacc atcaaagaac ggttttccat cgtgttcagc ataaaagcca
    14941 cgtgcctgtt caacggtcag gtgcagcatt ttggtgccaa caattttgaa ccctgcagct
    15001 tcaaagcgcg caaagatatt accaatgacg ttttttgcta ccgcgttcgg tttgatgatg
    15061 gaaaaagtac gttcaatagc catttttacc tctgtaaatt gttctgttgt tgtctgtacc
    15121 agcgtacgag atggcgcgga ttataatgag caacagggcc gttgactatt gatgaaggta
    15181 acattttttt aaaataaaat gagttttagc aacaatcagg gacaaaacag actattgcat
    15241 gacaaatttc actgtcgcga tttgccccac gtcatccatc accagcaatt gataatcacc
    15301 tttatccgtc aaatgcaggg tcacgttgcg cccgcgttca gttaacggtt cgccattcag
    15361 aaaccaccag cgttcacctg cccctccact tgattgcaac ggcaaagtcg cttccgctgc
    15421 gcccggtaaa cgtttaataa tcgcgccatc gcgaacgcct gtcagctgca gcgggagctg
    15481 ggcatcgtga ccgtatggcg ggcaacttgt cgaggccggt ggtaagcgca cagcgcgacg
    15541 ttctgatgcg ggcagccagg gttccagcgg tagcggccag acatttatca tttcttgtcg
    15601 cgcttgcggg caatcagcgg caacacgttt gccattttca tccagccaga tggggaaacg
    15661 aatgccatta atgccttcct gctccggcag taatagagtt ggcggctgac tcccgtccag
    15721 cagccaggtt gccaggcggc ggcgacagtt accgtcacct tccggcaaag actgtccgcc
    15781 cggccagcag ataacgccac gagtgactga gttcgggcgc gggtcttccg gcagattcgc
    15841 actgcgcgac agtaagatat tattgacctg attcaacaat ggtacggcac tggcaaagcc
    15901 aaactgacca acaacgggcg tgccgtccgg tctgccagtc cagatcccaa tgacatagcg
    15961 agcgttaacc ccaatcgccc aggcgtcacg atagccatag ctggtgcccg ttttccatgc
    16021 cagtggggcg acgcgcggca aggcactatc cggcaagggt tgcgcttcat cagccataat
    16081 ccggcgaatg atccacgccg cccccgacga cattaaaggc cgttcaagca gcggatcgtc
    16141 aggctgtaag cgcaatttgc ctgccttgcc gtggcgagca aacgcggtat acgctgccgc
    16201 catatcttcc agttttgcac cagcgccgcc gagaatgagt gaaagattcg gcgcagcacc
    16261 gttgggcaaa tataacggca atccaacatt gcgtaacttt gccgcaaacc gtttcggtcc
    16321 ataggcttcc agcacctgca cagcaggtaa gttcagcgag cgcaccagcg cctcgctcat
    16381 gctgatcggg ccatgaaaac cgctatcaaa gttacctggt cgataatcac cggtgcgccg
    16441 ggggacgtct tgcagcagtg atgccgggtg gatcaagcct tcatccagcg ccagaccata
    16501 aacaaacggt ttgagcactg atcctggcga tcggatcgaa ttgaccatat caacatgacc
    16561 aaagcgtgaa tcatcgttga gatcaaccga tcccacccag ccgcgaacac gcatatcggt
    16621 atgatcaacc acgatcatcg ccagtgagct gcgcggtggc aaccgccctt tccagttttg
    16681 cgccagttct tccagacgtc gttgaagacc ggcatccaac gtagtagtga ttttgtcgct
    16741 tttgctttta ccgagcatca tgcgcgaaaa cagcggtgcc agttgcggca tttgtcgggg
    16801 ggccagccag atgggttctt cccttgactc ttttacctgc tcacgggacc acacaccttg
    16861 cacggccatc cgttcgagca ctttattacg cgcggcttcg gcacgctccg gccaacgatc
    16921 cgggcgaaga cggctgggcg cttgcggcaa aaccgccagc attgccgcct cggaatagct
    16981 taaattcgca ggcgattttc cgagataagc ccaacttgcc gcaccgatcc cctgcaacgt
    17041 accgccaaac ggagcgcggt taagatacaa ggtcagaatt tcacgcttag acagatgcca
    17101 ttccagttgc aacgcgcgcc agagctggcg aattttgccg ccaaatgttt tggggtgagg
    17161 atcaagcaga cgagcaacct gcatagtgag cgtgctgcca ccggaaataa cccgtcccga
    17221 agtgagatct tgccatgctg cgcgcgccac cgagaatgga ttcacccccg gatgcttcca
    17281 gaaccagcga tcttcataat tgatcagcgc ttcaaggtaa cgtggagaaa catcttcgat
    17341 tgttaccgga taacgccaga tgccgtcagc atcggcgaag cgccagagcg gcgtaccatc
    17401 ctgcgccacg accactcgtg cgggattgac ttcatgcagc ggtagaggcc agagtttatc
    17461 tgctccccac gctgcaagaa aaaggagaaa gggcgcggct gccaacgtta tccagcagcc
    17521 gcgtttggtt aacaagcgag gcatttacgg tctgacaatc agcagatctt cagccgcgcc
    17581 ggtcgcccgc cattggggaa catacattga ttccaccatc ggttgcggta cctgatacgt
    17641 tcccggcgtc accgcccgcg ccagatacac cagcgttacc ggttggtatt catcaacggc
    17701 aaccgccgcc acaaagcgat cgtcacggaa ctcaatgtgc ttaatgctcg cctgctgcat
    17761 ctggttcagt aagttctgca cttcgccacc actttgctcc aggctggcgc taccgttcgc
    17821 cagattctgg ttttccagtt ccagacccgc aggcagcaga tccacgacta acgcatccgg
    17881 cacgctgtta ctggctttta cctgcaacca caccagcacc agatcgccgc tacgtaacga
    17941 gtccagcgat ttgctcttac catcagtacc aagaatatga cgctcgattt gcagcacatt
    18001 gttcgccggt aaaggtgcgg attgcggata accgctggca tccatacgca accataacgg
    18061 ctgatcgcca ctgttgctca cctgcaaggt gacaagttga tcgctattca gattgctgtt
    18121 ttgcgctttc tcgcctgtca gctgctcagc tgagaaagag gtttgcgcct gccatttacc
    18181 gggtaaatcc tgaatcgtac gggcagccag gaacaacgcg ttactttcct gcgtcgatag
    18241 ccagcgttca ccaaacgcct gctgcgaaag tgtgttcagc aaagtgtact gctcatcggg
    18301 tagcagttta ttttcttcca gcaaggagag cattaacgcg ttgtcgcgca gtgaactacc
    18361 gtaatcaccc agccatatcc gctcatcact attacgcggc gttttcagcg ccagcgcaat
    18421 cgcttcttca ccacgcgtcg catcacccat ggttttcagc gcaacgccaa gttgcagcag
    18481 cggtaaacca gaagcggcat ctgcacgatg ctcccagatt tcacgcagcg cacccagcgg
    18541 agccttttgc tgacgggcca acaccagcgc agcgtaagac tgtacggcga atttactggc
    18601 tttgagatta tccgcgtacg ggatcgacat catgcccgga tcttgtaaat agcgcagcag
    18661 acgctcatta ccccggttaa tggcgtctgt cggcacgctg taaccctgtt cgcctgcgcg
    18721 gaccaggaaa tccatcacgt aagccgtcag ccagtactct tcgtcaccgt ttttatccca
    18781 cagcgcaaag ccgccgttat cacgttgcat ttgcagcaaa cgggaaatgc cgatatcgac
    18841 cgatgcacgg cgtttctcat cactgtcgcc tttgatgccc aacgcctgca gttgggctgc
    18901 gttggtataa agtgacggaa acaggccgct ggcggtttgc tcaagacagc cgtacggata
    18961 cgcttttaac tctttgatat aacgtgcgat gttcagcggt ggtttgccgc tcaacaacaa
    19021 ttgcccttcc agcgtaacag gcgagaagtt ttgcaatcca tccgccggaa tcgcccatgt
    19081 ctcaccaggc tgtaacgccg taccgtaatt aaccgtttgt gccgggaacg ccggacggac
    19141 gccgattttc cactgcttat gctgatcggc aacggtttca cccggtaacg ctaacccgct
    19201 aatggtggcc tgaatttctc catcgccata acccggcaat gctcgcaccg ggataaacag
    19261 cgtagtacgc acacctggcg ctaattcaac ggctgcgggt gaatcgctga ccagttcaag
    19321 caaaccactg gcggtcaggg caacgttcag tttttgcggt ttatcggtaa gattagtgat
    19381 atccagcgtc agacgcgagg tatcgccact cgccataaag cgcggcatgt tcagttcagc
    19441 aatcaccggt gcggcaacta tcactttact ttcgttgcta ccgaagtcat ctgccgtcca
    19501 ggcttgcgcc atgacgcgca attcaccgtt aaaatcgcca atcggcagtg taaccgagcc
    19561 ttcgccctgt tcgttgagcg ttaccggcag cgcctgctgc gcgacaatat tgacgtgatt
    19621 gaccggcggt ttaccaccac gtttcagctc atcaccatcg ccaccgaaac gcagagctgc
    19681 cagacgcccc tgaccttcaa taacctgacc gtaaatatcg taaatgtctg cgccatagcg
    19741 tttctgacca aagaacgcct gccacggatc tggcgtgacg tagtcagtaa tattcagcac
    19801 accactatca acggcagaca ccagcacatt cacctgttta ggcttctcgc catttttagt
    19861 gctggcttta attttcacgg ttaatggttg attgggacgc atttttgctg gtgtttccag
    19921 cgccagatcg aggcgacggt tttcatcacc aagcggcaga tgcaacacac caaccgcgcg
    19981 ttttggcgtc gcggagcgag atttatcgcc aggacgcacc accagcgtac tcaaataaag
    20041 atcatgacga ttccaggttt tatcgaccgg aatcgtcaga tccagccctt gagccggaac
    20101 atcaatctct tgccaccaca gcggcccttc actggactcg accatcgcat aacctttacc
    20161 cgccgttggc gcggcaatat gcaacttaat ggtatcgcca gggcgataac tggctttatc
    20221 cagtttcagc gtgacacggt cgggtcgcac ggcaccgctc ccgtcgctgt tgtcctgcca
    20281 gctatagcca gcccagaaac gaacactact gaccgcttca ttcggcgctt tgacttccag
    20341 acgataagca ccccactcta ccggaaaact gaccttgccg gtttcgtccg ctttcagatc
    20401 cagagtttgt tcattttcga tcagatcttt ttgatcaaac tgtgactgcc agccttcatc
    20461 ttctgaccag ttccagtagt aatcgcggcg ttcgcgaatc aggcgcacct gcaagcccga
    20521 cacggctttt ttcacgcctt gcgcatcgct ataaacgatg tcaaaagcgg cgttactgcc
    20581 ttcatcaaca atgggctgtt tcaccgtgct gtcagtacga taatcgtaaa ccgatttcga
    20641 ggcgaactgc ggacggatcc ccggcaatgc atcggcaggc cagatagcct gctcagcgcg
    20701 gcgcgtcacc gggcgaccgc ccgattccag caaactaccc tggaaaataa cctgtaatgg
    20761 ggaatgcgtt tccttccact ggctttctgt agaaacttcg ccgcgccctt tatcatccag
    20821 cgtcaactga acttcatcca gcgtgcggga aaggttttcg gcagctatat cgccgaattc
    20881 aaaaccaggt aaggctgaca cagcctcacg cagtgggcgc aggaaaagtt gcccttgcaa
    20941 agtattacca ttagcaggcg caccgtacag gtagtatccc accacggaga atttcacttc
    21001 atcattcggc gtaagcgggg ttttctcacc ggtcagattc agcgccatac gctctggcat
    21061 aaaatcttcg acgtggaaat cccacatccg atactggtta tcgcccgtgt tagcgcgaat
    21121 atgccacata ccggttgccg cattgctatc gagtggccag gttaagtggt agaggccatt
    21181 ctccggctga ctaacgacgc tcctgagaac ctgcccgtcg ggtttaatca cgtctaactt
    21241 gatgggttga tcgggcaacg ctttaccgtc tgcatcacgc agcaaaccat tgaggattac
    21301 cgtttcaccc gggcgataaa gatcgcgcgg gccaaacatg aaaaactgtt tgctatagcc
    21361 tggcgcgcca gcaatgttaa attctgctaa gtccagcgcc ggaagtttta aatcgagtag
    21421 cgttgtctga ccgtctttac gcgccaacag taatgccgcg tttttatcat tttccagctg
    21481 cacatgcccc tgagcgtcac tggttgcctg agtcagagtc tgccctttct catttaataa
    21541 agagacttca attccttgct gggccgcgcc gttttccaga ctttgggtaa agatatccag
    21601 acgattgtga taacggtgag ctgaaacgcc gatatcactt aacgtaaaca gcgtcgcggg
    21661 attactgtaa tcgtaacgtc cagcctgatt catcacagcc agatacacgc ccgcctgctg
    21721 aagcggtttg atatcgccca gcggcagcaa taatttttca cgggtgttac gcgcaggatt
    21781 gagatcaaac cgtccggtgt agaccagatc cgccatctgc agcagtttgt ctgactgcca
    21841 gttcgccagc gaattgcggt attcccattg gctaatgaat gctggcagag attctggctt
    21901 aacgcgaaag aagttaacat cgacattatt aacgttgagc gccattaccg gcagcccttc
    21961 aacgacttta ccaggcagca gcgaaccacg gctggcaaaa ccgacgctgg gttggatgtc
    22021 gcgggtagtt atagtttttt cgtaatcttt actgaaggtt gcgttgttga gtgctttgac
    22081 ctccttgcca atagtaacga tcaaatcacg tttcggttcg aggtggcgta aacgcagctc
    22141 tttaagatta tctgacagct cccaggcacc atccactttg ccgctttttt tatcgacgac
    22201 atgaataacg cgtgagaaat cctgatccgg gtcgagaggg atggagaacg tcagcaccag
    22261 cgtggctgca ccatcaagtt ggacttcaga gagatccagc aatgtcagcg ccttaccggc
    22321 actctgttgt gccagttttt gtcgctctgg cgcggagagt tttgctgaac tcgcgttttc
    22381 tgaagaggcc gctttagtaa cttcagaagg cgcatctttt ttcaccgctg ttggcgcgtt
    22441 atcgttgttg tcgcaccctg ccagcgccag cattagcatg caggcggcta cgcgtaactt
    22501 tttcattttt catccctgcc acaatggccc gttagcaacg tcgaataatt attatgcgtg
    22561 agaatttacg ttatgtaatt cagtatcacg cccatacaat ccgaatttta gtgagaattg
    22621 tggctcagac agccaaatca gcctcttttg cttgtcgctt ttcactaaac atccgacaat
    22681 ttagcctaac cccggcaaaa atggagatgc ctatgtccac gacatggttt gtaggagccg
    22741 actggctcgc cgaacatatt gatgacccgg aaattcagat tatcgatgcc cgcatggcgt
    22801 cgcctggaca ggaggatcgt aacgttgctc aggagtatct gaatggacat attcccggcg
    22861 cagtgttttt tgatatcgaa gcgctttctg atcacacttc cccgcttccg cacatgctgc
    22921 cgcgcccgga aacgttcgcc gtggcgatgc gtgaattagg cgttaaccag gataagtacc
    22981 tgattgtcta tgacgaaggt aatcttttct cagccccacg agcatggtgg atgctgcgca
    23041 cctttggtgt agagaaagtg tcgattctgg ggggtggact tgcaggctgg cagcgcgatg
    23101 atctgctgtt agaagaaggt gcagtagagc tgccggaagg agagtttaac gccgcgttta
    23161 atcctgaagc cgtggtgaaa gtaaccgatg tattattggc aagccatgaa aatacggcgc
    23221 aaattattga tgcccgcccg gctgcacgtt ttaacgcaga agttgatgaa cctcgcccag
    23281 gtttacgtcg cggacatatt cccggagcac tgaatgttcc gtggacggaa ctggtgcgcg
    23341 aaggcgaact aaaaacgacc gatgaactgg atgcgatatt ttttggtcgc ggcgtcagct
    23401 acgacaaacc aattatcgtc agctgcggct ctggtgtaac ggcagccgtg gttttgttag
    23461 cactcgcgac gctggatgtg ccaaacgtga aactgtacga cggcgcatgg agtgaatggg
    23521 gcgcgcgggc agatttaccg gttgagccag tgaaataagt attttacagg caataaaaaa
    23581 ccgccgaatt tggcggtttt ttattgctag tctggttcgc ggcctttcca gcaggttgac
    23641 ttgtgttaca tgagcaacgc aggtgcttca cagcaaaaca atactcacca gtaactctct
    23701 ttttgtcaag caaaagagag taattattgt ttatttagcg tattatcgac accggccctt
    23761 tccgccgtgt tcggtaataa aataacctgg cttattagtc cgaattcaga caaatataaa
    23821 caaatcctgc tcaaaat
//
LOCUS       DAPHBJ010000057        23395 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.057, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000057 DAPHBJ010000000
VERSION     DAPHBJ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.057"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..76)
                     /locus_tag="RHZ76_001996"
     CDS             complement(<1..76)
                     /locus_tag="RHZ76_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDV2812642.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTA"
     gene            complement(134..1153)
                     /locus_tag="RHZ76_001997"
     CDS             complement(134..1153)
                     /locus_tag="RHZ76_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HDV2812643.1"
                     /translation="MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFTEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPIVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(1165..2379)
                     /gene="rspA"
                     /locus_tag="RHZ76_001998"
     CDS             complement(1165..2379)
                     /gene="rspA"
                     /locus_tag="RHZ76_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HDV2812644.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSANSAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(2585..2911)
                     /locus_tag="RHZ76_001999"
     CDS             complement(2585..2911)
                     /locus_tag="RHZ76_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HDV2812645.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            3046..3387
                     /locus_tag="RHZ76_002000"
     CDS             3046..3387
                     /locus_tag="RHZ76_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HDV2812646.1"
                     /translation="MKITLSKRIGLLAILLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            3422..3982
                     /gene="speG"
                     /locus_tag="RHZ76_002001"
     CDS             3422..3982
                     /gene="speG"
                     /locus_tag="RHZ76_002001"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HDV2812647.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(3985..4695)
                     /locus_tag="RHZ76_002002"
     CDS             complement(3985..4695)
                     /locus_tag="RHZ76_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HDV2812648.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            4803..5108
                     /locus_tag="RHZ76_002003"
     CDS             4803..5108
                     /locus_tag="RHZ76_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HDV2812649.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSSQDGAPAEPQ"
     gene            5307..6719
                     /locus_tag="RHZ76_002004"
                     /pseudo
     CDS             5307..6719
                     /locus_tag="RHZ76_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(7597..8586)
                     /locus_tag="RHZ76_002005"
     CDS             complement(7597..8586)
                     /locus_tag="RHZ76_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812650.1"
                     /translation="MMTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLTDRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYEKTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(8668..9159)
                     /locus_tag="RHZ76_002006"
     CDS             complement(8668..9159)
                     /locus_tag="RHZ76_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812651.1"
                     /translation="MMFNNNDWKLSVTDINPYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQQNKRAEAERIAKEQRIQAE
                     RMREENMTDAEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWL
                     DSL"
     gene            complement(9425..10183)
                     /locus_tag="RHZ76_002007"
     CDS             complement(9425..10183)
                     /locus_tag="RHZ76_002007"
                     /inference="COORDINATES: protein motif:HMM:NF016283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HDV2812652.1"
                     /translation="MSTQPLMKHEVVLPVIVDGYHWACNQDRMWNLNELHKELELPSA
                     KEPGQWRSSVRTLLDQDANLHVAHGGSTPGTWATEEAAIAYAMWVSPDFYLKVIRAFI
                     ALRNDKVSEAKALAQDSKELKGLKPIARNWLEKLDNPKQGHTLTECLKNLDFRLGTKL
                     VSAKALNAVLKSGRIANPFYSRKLDGRGQPVFDISSGGWMTSFNPKGLENGYYRLQDN
                     HYNGQEGLKVLTKGYEWLKSNKVELVRLCAKGGL"
     gene            complement(10768..11136)
                     /locus_tag="RHZ76_002008"
     CDS             complement(10768..11136)
                     /locus_tag="RHZ76_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812653.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLSESMTSNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            complement(11630..13228)
                     /locus_tag="RHZ76_002009"
     CDS             complement(11630..13228)
                     /locus_tag="RHZ76_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV2812654.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYLYQQVYTSSPDTRLTQIKDLAVP
                     QLVRRANGRYTIRFRLKGQTIPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPEVS
                     LQELTAHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGIRL
                     ALEVLTAAQQRVDTGDTSGLIKLIDGGVSQLTDDNNHTDYSTIGDSTSILKNEQGDRP
                     AVFTQERQPSVVFSSLVSSLLAEKVQTLKSSSYKDLSSSLNTVSRFLPEDMDLMSRSG
                     WLAVRDSMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRR
                     AFTDEELERLLVRVESEYQFTRHTAHTTSEARRWASLVSVVTGARSAEVCHLTKRDIV
                     TLDNGLVCIDINEDGDGKSVKNKHSVRLVPLTDGVCGFSLKSFLKWVDTQPADGPLFG
                     MTPSAYSSWFNSRVLTEALGDAENVSLHSLRHWLATRMKERGVNLVDAQGILGHSSQS
                     ITYDLYGKGHAVGRLAEVLKTALL"
     gene            13211..14320
                     /locus_tag="RHZ76_002010"
     CDS             13211..14320
                     /locus_tag="RHZ76_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDV2812655.1"
                     /translation="MVNVSHIRTLSSVESTTSSPSIRLTVESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLPDLMTVEQEDIIPNDYAGNMGYLI
                     FLKPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            14381..16804
                     /locus_tag="RHZ76_002011"
     CDS             14381..16804
                     /locus_tag="RHZ76_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HDV2812656.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEVPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            16815..17432
                     /gene="dmsB"
                     /locus_tag="RHZ76_002012"
     CDS             16815..17432
                     /gene="dmsB"
                     /locus_tag="RHZ76_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDV2812657.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            17434..18288
                     /locus_tag="RHZ76_002013"
     CDS             17434..18288
                     /locus_tag="RHZ76_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HDV2812658.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGVGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLFSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLRM
                     TVGMAIAG"
     gene            18331..18945
                     /gene="dmsD"
                     /locus_tag="RHZ76_002014"
     CDS             18331..18945
                     /gene="dmsD"
                     /locus_tag="RHZ76_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HDV2812659.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKCIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            19104..20396
                     /gene="clcB"
                     /locus_tag="RHZ76_002015"
     CDS             19104..20396
                     /gene="clcB"
                     /locus_tag="RHZ76_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HDV2812660.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(20349..21044)
                     /gene="bioD"
                     /locus_tag="RHZ76_002016"
     CDS             complement(20349..21044)
                     /gene="bioD"
                     /locus_tag="RHZ76_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDV2812661.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(21169..22389)
                     /gene="mlc"
                     /locus_tag="RHZ76_002017"
                     /gene_synonym="dgsA"
     CDS             complement(21169..22389)
                     /gene="mlc"
                     /locus_tag="RHZ76_002017"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HDV2812662.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(22524..>23395)
                     /locus_tag="RHZ76_002018"
     CDS             complement(22524..>23395)
                     /locus_tag="RHZ76_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2812663.1"
                     /translation="RYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLAR
                     TNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSSAPFIRAVS
                     DTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPESLEHAVIVHEPL
                     MAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEV
                     GEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPA
                     ARNFRIHLLNALR"
BASE COUNT         5838 a         5989 c         5812 g         5756 t
ORIGIN      
        1 gcgcagtgcc cagccatccc gatactgctg ctttcaccaa atccttagtg cttctttcgt
       61 gtttttctat tgtcataatg gttatctcta aaaaagaggt aagatgcgta ctacttactc
      121 gccgttattg gtattattca gaaaaagtga gtaagacttt gcagcaatgt ttttgatcct
      181 gttcaaataa actaatggca tcagcaacat gctggaaatc aaacgtatgg gtaattaatt
      241 tttctggttt aattaaccct ttacttaacc agtcgataac gatcgggaat ttatttgcat
      301 ttaagcgtga agagaaaata gagagttctt ttccggtaat tccttgctga atcacttcag
      361 acggttcact ggagaacccc atcaatacaa tacgtgccgc tggagaagcc agcgttacgg
      421 cctctttcag gatagaagga tgacaagccg catcgataat taatgtcggc ttgatgcctt
      481 tttcagtgaa aatctcgcca agcggtgtct ggctgttatt aatcgcccag tcagccccgc
      541 tctctttcgc tttttccagt cgttcatcaa tgcgatcggc aacaatcaca tttttaacgt
      601 tatagacgcc ttttaatacc tgaacgatcg tcaggccgat tggaccggca ccataaacca
      661 gaacggtatc attttcagtc ggttgaccat gtccggttac gttagccgca atggtaaaag
      721 gttcgatcat taccgcatat tgatcggcca ctgcttcagg aattttccac gcattttttg
      781 ccggaaccac ggcatattca ctgaaaccac cgtcagcgtg cacacctaat acagccagtg
      841 tcgtacaaac gttcggttta cctatagagc acggatagca atgcccacag ctgaccaccg
      901 gatcgacagc aacacgttca ccgactctgg cgctttccac gccttcaccc actgcatcaa
      961 tgacgccaaa gaattcatga ccaatgacgc gcggatattt cgcaaaagga ttatgcccac
     1021 gataaatatg gctatctgaa ccacaaattc cggcaagttt cacttttact cgtacttcac
     1081 ccgctgacgg ggtgggtatt tcacgttcga caatcgccag ttgattcggt ttttcaatta
     1141 atatgctttt cattatctta ctccttacca gttccacagc gtgccatctt ccagacgtgc
     1201 gactggtaga taagcaggtt catagggata tttcgccgcc agcttttcat cgaattcgat
     1261 accaagaccc ggtttgtctc ccggatgcat atagccgtta tcgaaagtcc agttgtgcgg
     1321 gaagacttcg agcatttgtt cggaataacc catgtattcc tggacaccga aattggggac
     1381 ccacagatca aagtgcagcg ccgcagccat gcagactggt gacaaatcgg aaggaccgtg
     1441 tgagccagta cgtacctgat acagcgaagc aaaatcggca atccggcgca taccggtaat
     1501 tccgcctgca tgggtcagcg tggtgcggat ataatcgatg agttgctctt caatcagttg
     1561 tttgcagtcc cagatgctgt tgaagacttc acccactgcg atgggtgtga cggtatgttg
     1621 gcgaatgaga cggaagcatt cctggttttc cgcaggcgtc gggtcttcca tccagaacat
     1681 gcgataatct tcaatgcttt taccaaagcg cgccgcttca ataggcgtta agcgatggtg
     1741 catgtcatgc agcaaatgtt cattaaaacc aaacttgtta cgtaccgcgt caaacaattt
     1801 cggcatgaaa tcgaggtatt tctccgtcga ccacagctgc tcttccggcc actgtccttt
     1861 ggttgcgggt tcataagcca gacctttacc tttcgacatg ccgtaggtgg ttttcatacc
     1921 agggattccg cactgcacgc ggatggcttt gaatcccagc tcctgatgac gggcataatc
     1981 atccagagct tcatcaatac tgtgaccggt ggtatggcaa taaaccatca ccccttcacg
     2041 agacgcgccg ccgagtaact ggtaaagcgg catgttggca gctttggctt taatatccca
     2101 cagcgccata tcaaccgctg aattggccga catcgtaacc ggaccgcgac gccagtaagc
     2161 acctttatag aaaaactgcc agatatcttc gatacggtgc gcatcgcgac caataagctg
     2221 cggacaaagg tgatcctgca aataagaggc cacggaaagc tcacgtccat tgagggtggc
     2281 atccccaagg cccgtaatac cgtcctcagt ggtgattttt aatgtgacga aattacgccc
     2341 cggacaggta acaaaaactt cagcctttac gatcttcatg ttcgattcct tgcatcgctt
     2401 gtcgtgatgc atgaaatcta cgcatctgag ctactaccat acaagtatga agattgaaaa
     2461 aagccggagt gatcacaaaa aaaggcgtat atttgcgctg tgaatggttg acaaaagatg
     2521 aaatagaata ccttttgtca gctgacactt cctcttatct tattgataaa atggatttat
     2581 gttcctacgt gcgcccccag cccgcaacaa tgatcaacat gccgcaaagc gcaatcaacg
     2641 cacccgtcca gtcataaaga gtcagtttca cgccatccac aacgcgcagc cacatcaacg
     2701 ccgtgcagac ataaacgcca ccataagccg cgtaaacacg cccactcgcc gctggatgca
     2761 acgttaacaa ccagacaaac agcgccagtg aaatccccgc cggaagcaac agccagatac
     2821 tggcgtttcg ttttaaccac aaccagggca gaaagcatcc aataatttca cacagcgcag
     2881 tagcaaaaaa tagtaacgtt gttttaatca tctttgtctc ttattgacat catgtatagt
     2941 tatagggcga cataatatca tcaatataaa caccctcctg gtacgttttg cgtccgcagt
     3001 ggatggtgta gaatcacctt taatcattca tacagggaat gaattatgaa aatcactctc
     3061 agcaaacgaa tcggcctgct cgctattctg ctgccttgcg cactggcatt gagcacaact
     3121 gttcatgccg aaactaacaa actggtgatt gagtctggcg acagtgcaca aagccgccag
     3181 cacgccgcta tggaaaaaga gcaatggaat gacacgcgca atctgcgcca gaaagtgaat
     3241 aaacgcactg aaaaagagtg ggataaagcc gacgccgctt ttgataaccg cgataaatgt
     3301 gagcaaagcg ccaacatcaa tgcctactgg gagcccaata ctttgcgctg cctggaccgt
     3361 cgaactggcc gcgttattac cccctaacct gttattgatt taaggaatgt aaggacacgt
     3421 tatgccaagc gcccacagtg ttaagctacg cccgctggag cgtgaagatt tacgctatgt
     3481 acatcaactc gacaataacg ccagtgtgat gcgttactgg tttgaggaac cctacgaagc
     3541 ctttgttgaa ctctctgatc tgtatgataa gcatattcac gatcagagcg aacggcgctt
     3601 tgtggtggaa tgtgacggcg aaaaagccgg tctggtggag ctggtggaaa ttaaccatgt
     3661 tcatcgccgc gcagaatttc agataattat ctccccggag tatcagggga aaggtctggc
     3721 aacccgtgcc gccaaattag caatggacta tggctttacc gttctcaatc tctataagct
     3781 gtatctgatc gttgataaag agaatgaaaa agcgattcac atttaccgca agcttggctt
     3841 ttcggttgaa ggtgaattga tgcacgagtt ctttattaat ggtcaatatc gtaatgccat
     3901 tcgcatgtgt atattccagc atcagtatct ggcagagcac aaaacaccgg gtcagactct
     3961 cctgaagccg accgcacaat agcattaata ataatcgatc gtatttttga tggtgtaaac
     4021 ccgttcgacg gcgggtttta ctccttcatc aacaatgatt agctgacagt ccaccggatt
     4081 agcgtgactg tcatattcac agctctgttt tacattacca acccgttgat tattcagtaa
     4141 agtaaccgct gtgtaatcta attttttgat cggatccgtt gatggcgtgg cgctgacaga
     4201 taatgttttg tcgttacttt tcgtggtttt acccagcgga taaccctgat catcatagcg
     4261 atattccatc tgcatttgtt tgctgctggc tttaatcacg aagccattat catcggtttc
     4321 ccaactcacc ccggcagaag gtaattctgc tagctggcat tttccctgta aacgtactct
     4381 cttctccagc gtctcggcat cacggtaata attggcgtcc agtaccagag cgaccacggt
     4441 attattttcc agatccagta attcgagtga atcaaaacag ccttcttccg acaaagtccc
     4501 agaaacacgt ttcgtcactt caccttgctc atccattaat gtctgagtga aatcttttac
     4561 cggaccacgc agcggatcaa aatcgaattc attagagaaa ctggccatct caggggtaaa
     4621 tgaaagtgtt acctctgtgc ggtcacatcc tgtgaggaat atcgcgagta agcatggtag
     4681 taatttgtat ttcacaacag tcaccagaga gtagagatga ttctcaatca tagtagcaaa
     4741 tacagtactt tacacgttaa atgctatgct tgaagaagtt atcttcgcgt aaggagctta
     4801 tgatgaaact ctcaacctgc tgtgccgcgc ttctgctcgc cctcgcctca cccgcggtac
     4861 tcgccgcgcc gggatcctgt gagcgcatac agagcgatat atcacagcgc attatcaata
     4921 atggcgtacc cgaaagcagc ttcacgttaa gtattgtacc caatgaccag gttgatcagc
     4981 ctgattccca ggtcgtcggc cattgcgcta atgatacgca taaaattctc tatacccgca
     5041 ccaccagcgg taacgtctct gctcccgcgc agtctagcca ggatggcgcg cctgccgaac
     5101 cgcagtaaca cattatcgac tgaacgccgg atatgacaca tccggcgatt tgaacataca
     5161 acataatccc accatattac tcataccctt ctattgatgt ggattaataa ttcttaaccc
     5221 aaaatgggta gactccctct attgttagcg cgctaaatat tcaatatata aacatttata
     5281 taacgataaa gaacagggag tgagttatgt ccaaaaatga acgaatggtg ggcatcagcc
     5341 gcagaacact cgttaaatct accgcgatag gttctctggc gctggctgca ggcggttttt
     5401 ctttaccgtt caccctacgc agtgcagcgg cagcggtaca acaggccagc gagaaagtgg
     5461 tctggggtgc ctgttccgtc aactgtggta gccgctgtgc acttcgtcta catgttaaag
     5521 ataatgaagt gatctgggtg gaaactgaca acaccggcaa cgacgagtat ggcaatcatc
     5581 aagtacgcgc ctgtttgcgc ggtcgctcca tccgccggcg tattaatcat cccgatcgct
     5641 tgaattaccc aatgaaacgc gtgggtaaac gcggtgaagg caaattcgaa cggattagct
     5701 gggatgaagc cctggatact atcgccagta gtctgaaaaa aaccgtcgaa caatatggca
     5761 atgaggctgt atatattcag tactcttcgg ggattgttgg cggcaatatg acccgctctt
     5821 cgccatcagc ctcggcggtc aaacgcctga tgaactgcta cggcggttca ctcaaccagt
     5881 atggctccta cagcactgcg caaatttcct gtgccatgcc ctacacctac ggcagtaatg
     5941 atggcaacag caccacggat attgaaaaca gcaagctggt cgtgatgttt ggtaacaacc
     6001 cggcagaaac ccgcatgagc ggcggtggca tcacttatct tcttgaaaaa gcgcgcgaga
     6061 aatcgaacgc caaaatgatt gttatcgatc cgcgatatac cgatacgact gctggtcgtg
     6121 aagatgaatg gcttcctatt cgcccgggca ccgatgccgc gctggttgcg ggtattgcct
     6181 gggtattgat taacgaaaat ctcgttgatc aaccttttct cgataaatac tgcgtcggct
     6241 atgacgaaaa aaccttaccc gcagatgcac ccaaaaatgg tcactataaa gcctatattc
     6301 ttggtgaagg tgacgataaa acagcgaaaa cgccgcagtg ggcttcgcaa attaccggta
     6361 tcccggtgga ccgtatcatc aaactggcgc gtgaaattgg cacagcaaaa cccgcttata
     6421 cctgccaggg ctgggggcca caacgccagg caaacggcga gctgaccgca cgcgctattg
     6481 ctatgttgcc tattctgacg ggcaatgtcg gcatcagcgg cggaaatagt ggcgcgcgtg
     6541 aatcgaccta taccattacc atagagcgcc tgccggtgct ggataatccg gtcaaaacgt
     6601 caatttcctg cttcagctgg acagatgcta tcgatcatgg ccccaaaatg acggcaatcc
     6661 gcgacggcgt ccggggcaaa gataaactgg atgtgcccat taagttcatc tggaacttca
     6721 aggacttgaa gcggaccctt agtaggcctg aggtcctgca ttgagtcaag gactttgtgt
     6781 caggctttgt gtcagccttg tgtcattaga cgggatgtct agacggctgg tcaggacccc
     6841 cggtggcctc ggtctggact tgaggcgggc tatgggggaa gtctgctggc ttaatgtatt
     6901 agatggtcat tcacattttt ctgtcagaaa ccatcggtcc cactgtgagt acaaacctaa
     6961 gcccgcagaa gcccacccac agccttagac agagtgtcga atacattcac accgagtcga
     7021 ccggagagga ccatatagag gcccacagag aggaacacga gtttatagac cagagtcagg
     7081 agggacctta ggtctgcgga agtcatggac ataaagcctc cttcgtctat tgtactactg
     7141 cactagataa ctagtacaaa acaaaaggtc cagaccattg gtccagacca tagttatcat
     7201 gatgattgga acgattgaac ggtaggcact ttacgtctca cccactgttc agcttcagcc
     7261 agtgaccagc atagccgctt atagactctg cgtgtcatcc agattgttgc ttgatatcgt
     7321 ccaccctcca caggctgtat agatgtctta atcacagtga caggccacca cctgaagtcc
     7381 tgctgatatt cagtccagac ttacctctgc tcatggtctt acctccctcg gtctcctgac
     7441 cttgggtatc gactacagct tgctgagcgt catctggagg agctacagga ggagctacag
     7501 gaggcgggac cgaagcccca ccttgagtcc caccaccacc tgtcaggccc ttggcgatgg
     7561 acctgaagcc tttactaatg ctcttaccca ttggtcttac gcttcggtct tggtgaactt
     7621 caggcgtttg actacaggca ggacggcagt agctgagcca gtgcccatct tgatggtcgt
     7681 agcagcttca gcgacaacga acactttacc gaggatttcc aagtcgtggg actgagagtc
     7741 gccatcacgt gaaaggacga ccgtaatatt ctcgccatcg tgacggaagc aaccgaagct
     7801 cagagccgtg aagcgtttag ccagcatgaa tacacccttg tcttcaccgt tgtagtaggc
     7861 gatgtctgtt cccagcatat cgacgatagc gtcttcaccg gagaagcctt gcttagcagc
     7921 cagacgggac agtgctttta gttggctctc atgagcgata ataccgtagt cagacacctc
     7981 agaaccgaaa gaagactctg tcttctcata cagagcgtcc atgatagcgt ctgccttagc
     8041 ggtcaggcta ccagtcagga caggaagctc tacggtcttc agcagcttag aggattccag
     8101 aacgtcgacc atgcgagcgt tgatggtacg gaccacattc tcacagatgt tcatagcgtc
     8161 ctgctgaagt gtgccctgag tgaatccggt ggatgacttg gcgtcccaga gcttggtctg
     8221 ctcagagatg agaatagcct gctcgattgg ttcgaccacg atttcttgtc cagacggaac
     8281 ggtcacgact tcaggtcgct tgccaccacg gacaggaacg accacagagc ggtcagtcag
     8341 agtgtgggca atgattgggg tcggtaagct gttacgggtc ggcaggactt ctacagggaa
     8401 cgggatgttc tcttgagcgt ccttaagtac accgttcagc atagccttaa ccgtcttagc
     8461 taactggagt gcgtagacct gcgggtcagc gttagtcggc agaccttcat tgacctgaat
     8521 gttgtcggta tcggtgatgc gtactggctc aatgccaaac tggaaagcgt ctttaatcgt
     8581 cataataatc aatctcctaa attagtttaa atactgtgag tcctcaaaac tgaggagtcc
     8641 ctaacatcgg gtcagggact gtgtgtctta taagctgtcc agccagttac cgcctgactc
     8701 aaggtcagcg acaatagcgg cacgggagcg accagcggaa tgggtagcac tgcggaggtt
     8761 ctcaccatag gtccggcgtt cagctttgac ctgttcgcgg cgctgtagct ctttctgcca
     8821 ctctgcatca gtcatgttct cttcacgcat gcgctcggcc tgaatgcgct gctctttagc
     8881 gatacgctca gcctcagctc gcttgttctg ctggcggcga ccataggcgg tcatcaggtc
     8941 attactgaag aagttgccaa tggtcacgcc agcttcagcc agagcttcca gtaactccat
     9001 cgcctcgcgg ctggtggacg gtaagcctga caggtagccc ggaatcaggg tcttaatcgc
     9061 cagagacagc ggataggact gaccgtccag actcacagta ttttcatagg gattgatgtc
     9121 ggtgactgat agtttccagt cgttgttgtt gaacatcata ggtaattctc ctcttgagag
     9181 ttgagactat cggtatcgca gacgcagtag cgcctacggg tctccccgca tcggtcatct
     9241 caaaaggttt taaaattagg cagacagttt catagcgacc agttgcgcta tcagctcgtc
     9301 tcgggattgg tgctggcggg tctcatcttc cgggtctaga ccaagctctc tgaggaggac
     9361 ttcagtccgg tagccacggt aacggagagt catgcggccc tcaggtgtgc gtgggtcaat
     9421 catcttagag tcctccctta gcgcacagac gaaccagctc gaccttgttg gacttaagcc
     9481 actcgtaacc cttggtgagg accttaagcc cctcctgacc gttatagtgg ttatcctgaa
     9541 gacggtaata gccgttctcc agacccttag gattgaagct ggtcatccag ccacctgacg
     9601 agatgtcaaa tacaggctgt cctcgaccgt ccagcttgcg actatagaag ggattagcta
     9661 tacgccctga tttcaatacg gcattaagag ccttggctga taccagcttt gtacctaacc
     9721 tgaagtcgag attcttaaga cactctgtca gggtgtggcc ctgcttcggg ttatccagct
     9781 tttccagcca gttacgggct ataggcttga gacccttaag ttccttggag tcctgcgcca
     9841 gcgccttagc ttccgacact ttgtcgttac gtaaagcgat gaaagcacgg atgaccttaa
     9901 ggtagaaatc aggtgagacc cacatcgcat aggcaatagc cgcctcttca gtagcccatg
     9961 tccccggagt cgaaccacca tgagcaacat gcaaatttgc atcttggtca agaagcgtcc
    10021 tgacgctaga acgccactga cccggctcct tagcagatgg aagctcaagc tctttatgta
    10081 gctcgttcag gttccacata cggtcctgat tacaggccca gtgatagccg tcaacgatga
    10141 caggaagaac tacttcatgc ttcattaagg gttgagttga catatcgttg tactcctttt
    10201 gttatgcctc ctgaagtcac accctgaaag cctactcaag cccccaacga tagggagact
    10261 cacaggcaac aggagacata cagaaagact tgaggtaata aggtcgtgtg aagaccatgt
    10321 taactttttt aacaaacccg ttttctgggt tggttagact tctcgccaac gcgcccaaat
    10381 ttggacccgt taagaatggt gactgctcag ttttgagcgc gttaagtcct gaccagacgg
    10441 aaactttttc ctactggcta ctagataact agcgtgtctc catatgtata aaggcgggac
    10501 cgcatgtcag gacgagctgt aaggacttcc agatggtgag cggtgataaa cttaagccaa
    10561 tgagtagtga gtagacgagc tactaacaga tgactgaaga acgctaacga gctggaggtc
    10621 gagacaggag gtctgtcaga ggcggtcacg ggccttgatt acttaggaga atacttaatg
    10681 tcggttgacc agatgctcgc atgttctgac gcatcagact cagaggcgga ggctggtctc
    10741 cctatagtga tagtttaggg tccgtaatca tgaaccagct ttactggcgt ggtactggac
    10801 ggatttactg gcgctgacga cggtagcata gacgtagcga gcgtagcgag ttgaaaccat
    10861 aggttcaccc aaggcaacac gcttacggct cagggactcc tgtacagcgc ttacagttaa
    10921 tgtgtcgtgc gttagtagtt gggtcagaat caagtatggg ctgacaggct tggtattgct
    10981 tgtcattgat tcgctcaggc gaccagcacc agtaaggaca tcatccagcc agccactcaa
    11041 gtcaccctga ccatcgaatg acagcaggtc cccgcagtgc tgctgcaagt aggctttggt
    11101 gaacgtgttg tcgtgagttg cgattggtcg tgccatagag tatctcctgt agttgaatta
    11161 gttggttccc ctatagtgat aatcaagtcc atcactggtg atgcatacag tagcgcgaca
    11221 ggatgtgact cacaggctgg actggatgtc acggattaag gcgaccctag gaattaagga
    11281 agtaaatata aagaggggga ccggatgtga ctcacaggtt gaaccgaatg tcaaggacta
    11341 aagacgcctc aggaattaag gaagtatata taaagagaga ccagaggcga ctcacagtta
    11401 aacaggaatg tcaagaggag accataggaa tttggaggga ccggagttta ggacgcagtt
    11461 taaataagac cacagacaga cttagagtcg gacagccgaa ggcggaccag tgggcctata
    11521 gtcaggaggg attatgatta ttatcaccct ccctaacttc agtcagactt cagtgaccca
    11581 aggtctcaac catcaagccg tatatggtct ccaccttggg tcttcctact taaagtaaag
    11641 ctgtcttcag cacctcagcc agcctaccaa cagcgtgtcc tttcccgtac aggtcatacg
    11701 taatggactg actgctgtga cctaagatgc cttgagcgtc caccagattg actccacgct
    11761 ctttcattcg ggtcgccagc caatgtctca gactgtggag agaaacgttc tcagcgtctc
    11821 ctagggcctc agtcaggacc ctagagttaa accaactaga gtaggcactc ggagtcatac
    11881 caaagagggg accatcagca ggctgagtgt ctacccattt gaggaaggac ttcaggctga
    11941 aaccacagac cccatcggtc agcggcacaa gtcggactga atgcttgttc ttcacagact
    12001 taccgtcccc atcttcattg atgtcgatac agaccagccc attgtccaag gttacaatgt
    12061 cacgtttagt caggtggcag acttcagcag acctagcgcc agtgaccaca gacaccagag
    12121 acgcccacct acgggcctca gaggtcgtgt gagccgtgtg tcgtgtgaac tgatactcag
    12181 actcaacacg gaccagaagt ctctccagct cctcgtctgt gaacgccctg cgcttggagt
    12241 cgatatcctt ggtcagcttc atccgttcaa tagggttgcg gccctcaagt tgcccattca
    12301 tcaggccata atcgagacac atcttcgctt tggtcagaag cttgttgatt gtcgatggtc
    12361 tcacctcaga agccagcata gagtccctga cagccagcca cccagagcgg gacatcaggt
    12421 ccatgtcttc aggcaggaac cgggagacag tattgagtga ggacgagagg tccttgtagc
    12481 tggaggactt cagggtctgg accttctcag ccagcagcga ggacactagg cttgaaaaaa
    12541 ctacagatgg ctgacgctcc tgtgtgaaaa ctgctggtct atccccctgc tcattcttca
    12601 gaattgacgt agagtcgcct atagttgagt aatcagtatg gttattatca tcagtaagct
    12661 ggcttacgcc accatcaatc agcttaatca gaccagaggt atcaccagtg tctaccctct
    12721 gctgtgctgc ggtcaggact tcaagcgcta accttatgcc cttctgctgg tccagactca
    12781 aggcttgcgt agcggctagc tccttcaggt tggacttctc gtcgaccaaa gtagcgacct
    12841 caagtccatt ccagtaatcg tcactagcgt ctgtcaggaa ctgctcagcc atagaccgga
    12901 ggtgcgctgt aagctcctgt agtgagactt caggcctatc caacatgaag gccttagctg
    12961 tggctgcaag ctctctctga cgcattgtgg ctactctcct gtctgtgctg cgggtcgata
    13021 ctgaaagaaa cggtatggtc tgtcccttga gtctgaagcg gatagtatat cgaccattgg
    13081 ctctgcggac taactgtggc actgcgaggt ccttgatttg tgtcagcctt gtgtcaggac
    13141 ttgaggtgta gacttgctga tacagatagg gagacccaga gaaagaactg gatggatata
    13201 cagtattgtc atggttaatg tcagtcatat caggacctta agtagcgttg agtcaactac
    13261 ttcaagtccg tccatccgtc tgacagtgga gtccatctgg aactacgcgg gaaataccct
    13321 cgttaatcag cattctgaca tcaacaaaac gcatgaaatt ctgcaggacg aatcgaaatg
    13381 cgaaatgatt gtggtcatcg aaaactttat gacctcatcg gcaaaatatg ccgacattct
    13441 gctgccagac ctgatgaccg ttgagcagga agatattatt cctaacgact acgccggtaa
    13501 catgggatat ctcattttcc tcaagcctgt caccagcgaa aaattcgaac gcaaaccgat
    13561 ttactggatc ctgagtgaag tcgcgaaacg tcttggacct gacgtctatc aaaagttcac
    13621 tgaaggtcgc acgcaggaac aatggttaca acatctgtac gccaaaatgc ttgccaaaga
    13681 tccggcgtta ccgtcttacg acgaactgaa aaaaatgggt atctataagc gtaaagatcc
    13741 caatggtcat tttgtcgcct acaaagcatt tcgtgacgac cccgaggcaa atccacttaa
    13801 aacaccttcc ggtaagattg aaatttattc cagcaagctg gcggaaattg cccgtacctg
    13861 ggaactggaa aaagatgaag tgataagccc actgccagtt tatgcctcta cctttgaagg
    13921 ctgggactcc cctgaacgta gaaccttccc actgcaactg tttggtttcc attacaaatc
    13981 ccgtactcac tcgacctacg gcaacattga tgtcctgaaa gcagcctgcc gtcaggaggt
    14041 gtggatcaac cctatagatg cgcagaaacg tgggattgcc aacggcgata tggtgcgggt
    14101 gtttaaccat cgtggcgaag ttcggctacc agccaaagtg acaccacgta ttctccctgg
    14161 agttagcgct atgggccagg gagcctggca cgaggccaat atgtctggcg ataaaatcga
    14221 ccatggcggc tgtgtgaata cgctaaccac tctgcgccct tcaccactgg cgaagggaaa
    14281 cccgcagcac actaatctgg tcgagatcga aaaaatataa cccactacaa ccataaattc
    14341 tggcatgaca ttttgtttga aaagcaataa gtgagtaatg atgaaaatcc ataccacaga
    14401 ggcgctcatg aaggctgaaa tcagccgtag aagtctgatg aaaacctccg cacttggcag
    14461 tctcgcgctg gcaagcagtg ctttcactct gccattttcc caaatggttc gggcggcaga
    14521 ggttccggta gaagagaaag cggtctggag ttcctgcacc gttaactgcg ggagccgctg
    14581 tctgttacgt ttgcatgtga aagatgacac cgtgtactgg gtggagtctg atacgacagg
    14641 tgacgacgtc tacggtaatc atcaggttcg cgcgtgttta cgcgggcgct ctatccgccg
    14701 acggatgaat catcctgata ggttgaaata tcccatgaag cgcgtcggca agcgcggtga
    14761 aggtaaattt gaacggataa gttgggacga agccctggat accatcagtg ataatcttcg
    14821 gcggatccta aaagattacg gcaatgaggc tgtacatgtc ctgtacggaa caggtgtaga
    14881 tggcggaaac atcaccaact caaacgtccc gtaccgtctg atgaactctt gcggtggttt
    14941 tctcagtcgc tatggcagct acagtaccgc acagatcagt gccgcaatga gttatatgtt
    15001 cggtgccaat gatggcaaca gcccggatga tatcgccaat acgaaactgg tcgttatgtt
    15061 cggaaataac ccggcagaaa cgcggatgag cggcggtggt gtcacttact acgtcgagca
    15121 agcccgcgaa cgttcaaacg cacgcatgat cgtcatcgat ccacgttata acgacactgc
    15181 tgccgggcgt gaagatgaat ggctgcccat tcgccctggc accgatggcg cactggcctg
    15241 tgcgattgcc tgggtactga ttactgaaaa catggtcgat cagccatttc tcgacaaata
    15301 ttgtgttggt tacgatgaaa aaacgctgcc cgccaacgca ccacgtaacg cgcattataa
    15361 agcctatatt ctgggcgaag ggcctgacgg catagctaaa acgccggaat gggcagcaaa
    15421 aatcaccagc atcccggcag aaaaaattat ccagttggca cgagagatcg gttcagcaaa
    15481 acctgcttat atttgtcagg gttgggggcc acaacgacat tccaacggcg agcaaacatc
    15541 ccgcgctatt gccatgcttt ccgttctcac cggcaacgtc ggcataaacg gcggcaactc
    15601 aggcgtacgc gaaggtagct gggatctggg ggtagaatgg ttcccgatgc tcgagaatcc
    15661 tgttaaaacg cagatttccg tctttacatg gacagatgcc atcgaccatg gtacggaaat
    15721 gaccgcgacc cgtgatggtg ttcgtggaaa agaaaaactg gatgtcccca tcaagttttt
    15781 atggtgctac gccagtaaca cattgatcaa tcaacatggc gacatcaatc acacccatga
    15841 ggtgcttcag gatgacagca agtgcgagat gattgttggc attgaccact tcatgacggc
    15901 ctcggctaag tattgcgata tcctgttgcc cgacctgatg ccaacagagc aagaagacct
    15961 tatctctcat gaatctgcag ggaatatggg ctatgtgatc ctcgcccaac ccgcaacctc
    16021 agcaaaattt gaacgcaaac ccatctactg gatgctgagt gaagtcgcca aacgcttagg
    16081 accagacgtt tatcaaacct ttactgaagg tcgcagtcag catgaatgga tcaaatatct
    16141 ccatgcgaaa acgaaggaac gtaaccctga gatgcccgac tacgaggaga tgaaaacgac
    16201 cgggatcttt aagaaaaaat gcccggaaga acactacgtc gctttccgcg cattccgtga
    16261 agatccacag gcaaacccgt tgaaaacacc ttcggggaaa atcgaaattt attctgaacg
    16321 actggcgaag attgcagata cctgggaatt gaaaaaagat gaaattattc atccccttcc
    16381 tgcgtatacc ccaggttttg atggctggga cgatcccctg cggaaaacct atccactgca
    16441 gttaacgggc ttccattaca aagcgcgtac ccactccagc tacggcaata ttgatgtgtt
    16501 acagcaggcc tgcccacaag aggtgtggat caaccccatt gatgctcagg cacgcggtat
    16561 ccgtcatggc gataccgtgc gggtatttaa caataatgga gaaatgctga ttgccgcaaa
    16621 agtgactccg cgtattctgc ctggcgttac cgccatcggg caaggtgcgt ggcttaaagc
    16681 ggatatgttt ggtgaccggg tcgatcacgg cggcagtatc aatattctga cctctcaccg
    16741 cccttcaccg ctggcaaagg gaaacccgtc gcacagcaat cttgtccaga tcgaaaaggt
    16801 ttaaggagta gcccatgacc acacaatatg gattttttat cgattccagt cgctgtactg
    16861 gctgtaaaac ctgcgaactg gcgtgcaaag attttaaaga tcttggcccg gaagtcagtt
    16921 tccgccgtat ttatgaatac gctggcggcg actggcagga agataatggc gtctggcacc
    16981 agaacgtgtt cgcttattat ctctccatct cctgtaacca ttgcgatgac cccgcatgta
    17041 caaaagtctg ccccagcggc gcaatgcata agcgtgaaga tggctttgtg gtggttgatg
    17101 aagatgtttg tatcggctgt cgctactgcc acatggcttg tccgtacggc gcgccacagt
    17161 acaatgctga aaaagggcac atgacgaagt gcgatggttg ttattcgcgc gtcgccgagg
    17221 ggaaacaacc catatgtgtc gaatcctgcc cgctgcgtgc acttgagttc ggtccaattg
    17281 aagagctgcg ccagaagcac ggcactcttg ctgccgtcgc gccactgccg cgcgcgcatt
    17341 tcacaaaacc caatatcgtt atcaaaccta acgccaacag ccgcccgacc ggcgatacca
    17401 ctggttatct ggctaatccg gaggaagtgt aaaatgggaa atggatggca tgagtggcca
    17461 ctggttatct ttaccgtatt gggtcaatgc gtagtaggcg cgctgattgt tagcggaatt
    17521 ggctggttcg ccgccaaaaa tgacgcagac cgtcagcgta ttgtccgtgg catgtttttc
    17581 ctctggctgt tgatgggcgt cgggtttatt gcctctgtca tgcacctggg atcgccactg
    17641 cgggcgttta actcgctgaa tcgaatcgga gcttctggtc tgagtaacga gattgctgcc
    17701 ggttccatat tttttgccgt tggcggatta tggtggctgg tggcagtcat cggtaaaatg
    17761 ccgcaagcgt tggggaaact ctggttactt ttcagcatgg cgctcggcgt cattttcgtc
    17821 tggatgatga cctgcgtgta tcaaatcgac accgtgccaa cctggcataa cggttacact
    17881 acgctggctt tcttcctgac ggtattgttg agcggtccaa tacttgctgc cgcaattctg
    17941 cgggcagctc gcgttacatt taataccaca ccatttgcca tcattagcgt tctggcgctg
    18001 atcgcttgtg ccggggtgat tgtgctgcaa ggtttgtctc tggcgtctat tcactcatcc
    18061 gtgcaacaag ccagcgcact ggtaccagat tacgcctcct tacaggtctg gcgtgtggta
    18121 ttgttatgcg caggtcttgg atgctggctt tgcccgctca ttcgtcgccg tgaacctcac
    18181 gttgccggac ttattctggg gctaatcctc atacttggcg gtgagatgat cgggcgtgtg
    18241 ctcttttatg gcctgcgcat gacagtagga atggctattg caggttaaca aattggtgcg
    18301 gggcaacccg cacatttcag gatgttagga atgacccatt tttcacagca agataatttt
    18361 tctgtcgcgg cgcgcgtgtt gggcgcgctg ttttattacg ctccagagag tgcggaagcc
    18421 gcacctctgg ttgcggtact caccagtgac ggctgggaaa ctcagtggcc tctaccagaa
    18481 gcgtcattag cgccgctggt gacggctttt cagacacagt gtgaagagac tcacgcccag
    18541 gcctggcagc gtttgtttgt cggcccgtgg gcactgccgt ctccgccgtg ggggtcggtc
    18601 tggctggatc gcgaatctgt gctgtttggc gattcaacat tggcacttcg tcagtggatg
    18661 cgcgagaaat gcattcagtt tgaaatgaag caaaacgaac cggaagatca ttttggttca
    18721 ctgctgttga tggctgcgtg gttggcggag aatggtcgcc agacagaatg cgaagaattg
    18781 ctggcatggc acctttttcc gtggtcaaca cgttttcttg atgtttttat cgaaaaagca
    18841 gaacaccctt tctaccgtgc actgggtgaa ctggctcgct taacgctggc gcaatggcaa
    18901 tcacaactgt taattcctgt cgcggttaaa ccgctgtttc gataggatca ccgtaatatt
    18961 gccggatggt gacgattacg cgcaatccgg caataatagg ttacagtgtc acgttttttt
    19021 atctcttaaa gcacgcactg cttttgcggc tggcctcttt tgccgcaaaa tagtcgcccg
    19081 tgtttcattg cccatttctg ctcatgcatc atctacacat ctatccggat ctgcgcacta
    19141 tgttccgccg tctgcttatc gcaacagtcg tcggtattct cgcggccttt gccgttgccg
    19201 ggtttcgtca tgcgatgctg ctactggagt ggttgttcct caataatgac tccggcagtc
    19261 tggtcaatgc agcgacaaac ctttccccct ggcgacggtt gctaactccg gcgctcggcg
    19321 gactggcggc gggtttgttg ctgatgggct ggcagaaatt tacccaacaa cgccctcatg
    19381 cgccgaccga ttacatggaa gcgttgcaaa ccgatggaca gttcgattac gcagcaagcc
    19441 tggttaaatc gcttgcctct ctgctggtag taaccagcgg cagtgcaatt ggtcgcgaag
    19501 gtgcgatgat tcttttagct gcccttgccg cctcctgttt tgcccaacgt tttacgccac
    19561 gccaggagtg gaaattatgg atcgcctgtg gggccgcggc gggaatggct gcggcctatc
    19621 gtgccccgct tgctggcagt ttatttatag ccgaagtgct gtttggcact atgatgttgg
    19681 cctctctcgg cccggtgatt atttccgccg tcgtggcatt gctggttagc aatctgatta
    19741 atcatagcga cgcgttactc tacaacgtac aactctcagt gacggttcag gctcgtgact
    19801 atgcgctgat tatcagtaca ggtgtgctgg caggtctgtg cggaccactg ttgttaacgt
    19861 taatgaacgc ctgtcatcgt ggatttgtga gtctcaaact tgcgccgccc tggcaactgg
    19921 cactaggcgg gttgatcgtg ggtctgcttt ccctgttcac acctgcagtg tggggcaacg
    19981 gctatagcac cgtacaatcc tttttaaccg ccccaccact gttaatgatc attgccggga
    20041 tcttcctttg taaactgtgt gccgtgctgg cgagtagcgg ttccggcgca cccggtgggg
    20101 tctttacacc gacgctattt atcggtcttg ccattggcat gttgtatggt cgtagcctgg
    20161 gattatggtt ccctgatggc gaagaaatta cacttttact cggattgacc gggatggcga
    20221 cactgttggc ggcgaccacg cacgcgccga ttatgtcgac gttgatgata tgtgaaatga
    20281 ccggggagta tcagctactc cccggtttat tgattgcctg cgtaattgcg tcggtaattt
    20341 cgcggacgtt acaccgtgac tctatctacc gccagcacac tgcgcagcat agctaagcgg
    20401 atgtattgcc ccagttcacg ctgttcggcg cgcggcagat aaggcagttc accaatgagc
    20461 ggtgccggaa gttttttacc cagcacatca atgatttccg cataatgcgc cagtcctggg
    20521 ttgattcggt tagccaccca gccaatgagc ggcagcccgt cgttggcgat cgcctgagct
    20581 gttagcagtg catggttaat gcaaccttcc tgaataccga caaccatcaa caccggcagt
    20641 tgttcctgca ctacccattc agagagtgga cgcaaatcat tcatcagact gcgccagccg
    20701 ccagtccctt ctaccacgac atgatcgact ttttcggtca ggtttgccag gccgtttgaa
    20761 atgagggtgt aattgattgg gcaactgtgc gccacgctac tttcttcttc gcttaacgcg
    20821 ataggattaa ctgcttcata aggcagttcg atggttgaaa cactctgcaa caccagggca
    20881 tctttattac gcagcccttc gggtgtctct ttgctcccct tcgctacggg tttatatccc
    20941 gcaaccgttt ttccctggga ggctaacgct tgtagcaatg cgcgggaaac caccgttttc
    21001 cctacagaag tgtctgtacc ggtaataaag aaacgcttca gcatcactaa ctccaccgtt
    21061 atgcttcaca aatataaacc aggaaaataa ttaaccttga aagtctaagt tatgctttcc
    21121 tggcccaaat tgagatagcg caaattttgg tagaacagtt aaaaaatgtt aaccctgcaa
    21181 cagacgaatc aacaaagaac cgttatacat cgcgtctttt accagtgcag cgcctgccat
    21241 cgtgccctgg ttagaaaact gagtactctc aacgctgatg tgctgactat acgcaggaag
    21301 ggcctgctga cggatgctgt ctgagatgac cgggaagagg atatctgccg ctttacttaa
    21361 cggtgagcca atcagtattt tttgtgggtt aaataaattc accatgatgg caagaatgcg
    21421 cccgacatgc gcgcccaccc cggtaatgat gtcttttgcc agtagatcgc cgcgcaatgc
    21481 cgcctgacac aatgagtcca cggttaacgg ttgtccatgt aacatcgagc tcatggattg
    21541 attaagacgc agctgtgcca gctcaagaat actgtccacg ctggcgatgg tttcgaggca
    21601 gccgtgattc ccgcaataac agcgtttccc atacgggtcg acctgtgtgt ggcctatttc
    21661 cacgagacta ctgctgcctg cgtgtagcag atgaccatcg gtaatgacgc ccgcccccac
    21721 gttgtgatcg ataaccacct gaatcacatc gcgcgccccg cgtgaggcac caaacaaggc
    21781 ctctgccatc gtccatgcgc tgatatcatg ctgaatataa accggaacgc cggtatgctg
    21841 ctccagcgcc tcgccgagcg gcatctcttt tacatcctcg tagaacggca tgcgatgtac
    21901 aataccattt tccgtatcaa taattcccgg caaggttatg gcaatcgaag ttagacgctc
    21961 aagttttttc tggtggcgga taaaaaactg atcgatatgg gaaataatac gatccagcaa
    22021 tggcaagtca tcttttaacg ccagttcctg cgactcttcc accaccagtt tgctgctcag
    22081 atcgcgcaga gcaaggaaaa tctccccgcg actaatgcgc agagaaagat agtgccaggc
    22141 ttcagtttca accaccagcc ccaccgccgg acggccacgg ttccccgctt ctttgatttc
    22201 cagctcttgc accaggtgtg cttcgagcat ctcacggaca attttagtga tactggcagg
    22261 agccagttgc gccagacggg aaagatcgat acgcgagact ggaccaagct gatcaatcag
    22321 gcgataaacc gcgcccgcgt tggtctgctt tatttgatca atgtgcccag gctggttttc
    22381 agcaaccacc gcatactccc tatattttcg cgctccgaaa taatctgtag gctatggtga
    22441 agcacttcaa tacgtgtcgt caaattttta cttaggcatg tgattaacag cacatttttc
    22501 gggctttttc gctgaaattt ccctcacctg agagcattca gcagatgaat acgaaagtta
    22561 cgcgcagccg gactttgttc atgatgtttc ggccagacca accacatttc agaaaccgca
    22621 tcctcttcag caatcggcac ccagcgcatt tcgttgagct gaacacgttt aaatgacgca
    22681 ggcaaaattg aaacacccag accggcggaa accagaccga tgatggtcat tgcctcgccc
    22741 acctcctgag tgatgacggg cgtcaaatgg taacgtcgca tcagcccgag aatatcgtca
    22801 tacagccctg tcccgacgtg cggatcaaaa aagacaaagg gttctttcgc cagttcagcc
    22861 agcgttacat tcgggttatt tgccagggga tgatcgtgcg ggatcatcgc cataagcggt
    22921 tcatggacga tgactgcgtg ttcaagcgac tccggtaacg ctgtgttacg cagcaatccc
    22981 atatccagcg ttccttcaat gagcggagcg atttgctcgc gagtgttcat ttcgcgggtt
    23041 tgtaaatgga catcaggata atcacggcga aacagcgata acgtatcgga caccgcccga
    23101 ataaaaggag ccgacgaagt aaaaccaatg cgcaactccc ccgcttcacc ctgatgcagc
    23161 ctttcagcgc gagcggcagc gtcatccacc atagacagga tttgccgact atctgcaaga
    23221 aactgttttc ctgctgccgt cagcaataca ctgcgattgg ttcgtgccag cagtcgggca
    23281 ccaatttgtt gctccagcgc ctgaatctgc tgacttagcg gcggttgcga aatattcagg
    23341 cgggcagcgg cgcgcccgaa atgcagctct tccgcaacag caacaaagta acgca
//
LOCUS       DAPHBJ010000058        23366 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.058, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000058 DAPHBJ010000000
VERSION     DAPHBJ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.058"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            43..684
                     /locus_tag="RHZ76_002019"
     CDS             43..684
                     /locus_tag="RHZ76_002019"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812664.1"
                     /translation="MRIKHLIFLPVPFISFYLNAASLDEINQKLDEGYRYACNATQTG
                     NAEAASLALENYRTKFINSASRSGFSEIMNVEINKVVAEFFRKGMIQGNTSTGACETP
                     AELLLQTSQAVYSRQSKSALASALDGVRFTEYAAQAYPIFYSIQSTGISEDEVIEQIN
                     KIGNNAIINNQNAIDVSNATNAMKEVAKVLFSARSDPELMKFMMDYINNHKLH"
     gene            964..2037
                     /locus_tag="RHZ76_002020"
     CDS             964..2037
                     /locus_tag="RHZ76_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812665.1"
                     /translation="MRKSYTYGIPFGLHRDTGRFLDITEVSRGSACNCICPGCRTDLI
                     ARQGEVKLWHYSHSTDLLGDCDGLMEAIRGKIIEVIQEKQLLGFPHLLAGYDGGTVPL
                     DEVSGSGSMFGATADLFVKVNDLCIAVFLDIDRSVAGKLTFDHLHASEMVAALRIDLP
                     DIEYEISQVQLGRRDGTYSECIEKIIIDATESREWLYHPMMQELGTEPLKVYEGREPA
                     EAGPLLQRLSDSTLKLPDNLPQSMNVLIRMQQATIVCLCRLTVRASDIAQTDEFPLFM
                     RYFRLHCLDTSEHVNYEKLLNWQDMITKSKEGQLLTVEERDYLQEIVRIGLYNHRLKS
                     VCLTACEDNRVGESEGPKNYSLL"
     gene            complement(2294..2770)
                     /locus_tag="RHZ76_002021"
     CDS             complement(2294..2770)
                     /locus_tag="RHZ76_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812666.1"
                     /translation="MEKTQSPAERFYESIYYSDESLEEDYLAELRNFSSDHWDTALRA
                     ARLSAAVKRFKTSEMLRFILEFVVPENAQEDVPDLTPLAAKRLCNSLFGRSGSQSILV
                     YVFGQAGRVHRSATCSPKTIEAIADLYRSDAERYWNSTLATIERVKHTYRAKIRNS"
     gene            complement(2850..3080)
                     /locus_tag="RHZ76_002022"
     CDS             complement(2850..3080)
                     /locus_tag="RHZ76_002022"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812667.1"
                     /translation="MSYAHLFENCDHVIEGNILLDIASTPPVLKKALIFLIQSGLERE
                     AFNAQLTELIKHLEFDITAAELSAHLLTPGNA"
     gene            3465..3857
                     /locus_tag="RHZ76_002023"
     CDS             3465..3857
                     /locus_tag="RHZ76_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000341068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812668.1"
                     /translation="MDDKEQFTSLVAKHASRLTEEQLAGYDDCSQYGECVSPSYEVFR
                     GYRTRHTLDEFLELAISLNAIHPDEYLTDMLLKPHEVIGALADEGDQLNNATPVYIFP
                     DTGVYAAAVSETRVLDAWLCWPCYPANW"
     gene            4879..5256
                     /locus_tag="RHZ76_002024"
                     /pseudo
     CDS             4879..5256
                     /locus_tag="RHZ76_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209044.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            5353..6180
                     /locus_tag="RHZ76_002025"
     CDS             5353..6180
                     /locus_tag="RHZ76_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HDV2812669.1"
                     /translation="MSGTFKGLTQAQIDRRLKDGRGQGQGSDYKPFIYTRDVSSLGRS
                     HRLPGSKTRRLHHLLSDLELAIFLTLDRSPHVTDIREQFPMRVEDTVRIAEELGLPHG
                     CYKGTPQVLTSDFLVDFEDPQRPSIAIQAKYSTDLQKPEVIERLELERRYWQEKGIPW
                     VIVTEREVSKVAFANIQWLYPAHAEGDIAQDDLAHYQQLFLLEFQRHPDRKLTAIAQG
                     LDTSGQLEAGQALYWLRQLLARHCFLFDLDTPYRELKPKDLVANSHQMHQELSSVAR"
     gene            6194..8254
                     /locus_tag="RHZ76_002026"
     CDS             6194..8254
                     /locus_tag="RHZ76_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017781027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2812670.1"
                     /translation="MGELRKRLLWSGTEQAIWIDIDSDTALPEPISIVELERLIMERE
                     LESIADPFEETVLREVEEGSLDQQKRDEAWGMLADFVHNPQLFVRRPRGLIVRGIMER
                     HGVTNQTVYRLLRRYWQRGMCRNALLPDYVNSGARGKRRKPNQAKLGRPRVVMEGKGS
                     NVTPDIERIFRRVIEERLLKEKHPSIPDAYAAGLNLLRAVLTELPTSELPTLGQFRYF
                     YGREYHFTDTLPCRVSAVDFAKDFRPLSSTSTTEALGPGYRYQIDATIADIYLVSEHD
                     RSLIVGRPVVYMVIDVFSRMVVGMYVGFEGPSWVSAMVALANTVADKVEYCRQYSVEI
                     DASDWPVKGLPDVVLADKGELNGTKVEAFSQAFGVRIENAPARRGDAKGIVERYFRTV
                     QERFKPYASGVVEDTTSRKRGGHDYRLDASLTLPEFTQIIIAGILYHNNFHTLSKYDR
                     TSGMPGDLPAIPVMLWNWGLANLTGRLRTAPEELVRINLLPHESATVSELGIRLFGCF
                     YTCQEAIREGWFHRGQGRRPTGVTVAYDPRSADHIYLRPSNSLKDYWVCDLADRSRRF
                     RGMTFWDVWLLSREERRSDTNAAAEALVERGNLLKQIESIVAQAEDASPLKTGMTTKD
                     LGTQIRENKQQEKRQERQKTAFKPDKAQQEKPAEVIPLRGEKQEDYAFPDLSDLIFKE
                     EEDD"
     gene            8247..9857
                     /locus_tag="RHZ76_002027"
     CDS             8247..9857
                     /locus_tag="RHZ76_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017781028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDV2812671.1"
                     /translation="MTEEFQRGVIPLARYQEQQLPEYQDNPLISALPPIPDLQEVVDL
                     LQQLPGFEPQEALLDGRLRAHAIARLLHGFFQPLSHHLELESKISLMIRQGYIGRNPA
                     SGAWYAHLQNGYRRVEEEDLDAAVYQSVSSTANSLSLFGCSGCGKTRTLERILGMYPQ
                     ALHHPDYDITQLVYLKIDCPIDGDLDELCLSFFNEVDKILGTHYSRSHGRKKLGTKRL
                     LASMCQLANLHTLGVLIIDEVQNLNEARSGGAEKMHNFFVSLVNTIGVPIIQVGTHRA
                     SKFFQRTFRAARRVSGLGSIRWDRLPRDEHWKRLLKRLWKYQWLRNAAPLDDELEATM
                     YDLTQGVMDIVIKLFALAQIRAIVTGVECIKPLLLRRVFEDEFKPVHPMLAALRSGRP
                     ELIAKYDDLLMPEIEGRLLTLTRSLDSVPQPKPPAPPADDKAKRLAALLEGMEIPRDI
                     AIPMADELLAENPDIPLAALIHKATAYLAQDAPKRPGISRVKRTEWGCLSEEDLRRRY
                     ADGPESEAYDRFKQAGLILDLRPLLRAS"
     gene            9859..11361
                     /locus_tag="RHZ76_002028"
     CDS             9859..11361
                     /locus_tag="RHZ76_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HDV2812672.1"
                     /translation="MYFPRLYPDELLYSGIARCRVHLGIGSHKTLLHMLFGDSKVAAI
                     TDLPTHLTALANNTGLDAEGMILNHTLFPLYAPFIPPERRTDLFQAMLAPDRPTIGLS
                     GASTALVKWPDWLRYCPICFEDMAARYGEPYWRRSWQIQGIDACPEHGCQLLNSPIPF
                     RRAQRHEFHTASPLFLPRDLRVSPVGEEAIRLAKAAMQLLALEEVQSPGYGRWTNLYR
                     YLATECGARRGRQVRAEVIWEKILASHQRDWLAANGLLTSGEPPPWLLAMFRKHRKGF
                     SALQHLIVWTSLRPGQHAGELISEAKTRQVDLASEWSVQLLPAEFEQKQQYRAIWLQA
                     LANHGGAKAARQDGAGACYAWLYRHDRHWLMVANQVRQRRQGNNSHIDWRARDIRLVR
                     LLIRIGRGSEEDLGLPRRSRNWFLQQLPHRASVEHRLDQLPLCCTFLDRYAESVGEYQ
                     IRRLTAAMLKDVQTGITSRRWELEKRCGLEKSRVAPLTTAFIRLIGRWIE"
     gene            11354..12976
                     /locus_tag="RHZ76_002029"
     CDS             11354..12976
                     /locus_tag="RHZ76_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2812673.1"
                     /translation="MNRTVFRQIPNGSRLLAVGNFFRAKTPAAQWQLAALFERNGQLQ
                     LQRFAQEMSCVLAVGREFLGEEGAPYRPSGFKKTVVLPAIDTWRERQLGECPRLARRL
                     AANPEISGQRCFVFEVDGLTVWLPKFELARKMFFHAAFIVRAAFAPNGLDMAFTIYRE
                     GDVVHIHTPPKTGAPSQLLKIKGYRDHFSWLLLNQDVRRSFESIWQSLNQRQERVLQE
                     SAYARWQFDFQAPTSLAGTTMDVRGPFDPESNELLVWEIEKLQGLRFSHRGDIFFHHP
                     ALKLPVRGENTGCVSVPSGAKELEVDADEEPTAAKEQRLIHLPVEGVTFDAQLRTKIA
                     YNGERANGHGKKVDDEQCPSRESQLFGVADPVSEGSIASGEFQQLGEGEGDGDEHERL
                     ADRFSLLREILEQITQESGVALVALEVRPLPPVPRCSYHMIDHEKSRCYLLVRFKLQN
                     GDQRYLLEIDTSDNRKTMSTRIMGFKAGVEAGKCIDRILRGTVKGSLRWPGTMAKYCE
                     PLHSVHHPKESSSGANHARVFDWKQRIRAALG"
     gene            13068..13169
                     /locus_tag="RHZ76_002030"
                     /pseudo
     CDS             13068..13169
                     /locus_tag="RHZ76_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(13460..13612)
                     /locus_tag="RHZ76_002031"
                     /pseudo
     CDS             complement(13460..13612)
                     /locus_tag="RHZ76_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010955146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(13569..13832)
                     /locus_tag="RHZ76_002032"
     CDS             complement(13569..13832)
                     /locus_tag="RHZ76_002032"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2812674.1"
                     /translation="MPPQRRTYSKSFKAQVIQECSEPGASIANIALGYSLNANLVHKW
                     IRLHTQKTTAIQPAFIPLPSQMLGAGSHAQVRCCDHEFDPVGP"
     gene            13847..14110
                     /locus_tag="RHZ76_002033"
     CDS             13847..14110
                     /locus_tag="RHZ76_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812675.1"
                     /translation="MGHGLRRRCREGVLAGRILLNYVVWGNGGFSARLWNAIRSDDWA
                     IPHIGLSSLGEIVGWARPDEFPPRNMRTSKGLRALGYNVRIGV"
     gene            14338..14619
                     /locus_tag="RHZ76_002034"
     CDS             14338..14619
                     /locus_tag="RHZ76_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV2812676.1"
                     /translation="MTARQQAIFTLDELATYLKVGKRTLYRLAAHGEIPAFKVGGTWR
                     FRQSEIDRWINDQIQAGRKKEVIRTDEQPKSAKHPVLPGRAIHSRRQTE"
     gene            14655..15224
                     /locus_tag="RHZ76_002035"
     CDS             14655..15224
                     /locus_tag="RHZ76_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003847761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDV2812677.1"
                     /translation="MDIDFKKLAPWNWFKKEQEEQQSTASLPVQRNDLPVAGGPVSPI
                     LQLHREIDRLFDDTFRGFGFPALAMPRWPSDWPGLLKPALDIQETDKQYKISLEVPGV
                     EEKDIHITLDNDVLLVRGEKRQEQESKDGGFHRVERSYGSFQRALNLPTDANQDTIKA
                     AFKNGVLTITMEKREASAPKQGRSIPING"
     gene            15322..18171
                     /gene="clpK"
                     /locus_tag="RHZ76_002036"
     CDS             15322..18171
                     /gene="clpK"
                     /locus_tag="RHZ76_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock survival AAA family ATPase ClpK"
                     /protein_id="HDV2812678.1"
                     /translation="MARKQCQVCGQPATVRVEANLNGRHSTMLLCDDHYRQLARQQKR
                     TVSPLEALFGSRSGLFEDFLGSDFFRIGDDSPSMAADTDEVVDASFGEPAPAGTGTAR
                     RRGSGLASRISEQSEALLQEAARHAAEFGRAEVDTEHLLLALSDSDVVKTILGQFKIK
                     VDDLKRQIESEAKRGEKPFEGEVGVSPRVKDALSRAFVASNELGHSYVGPEHFLIGLA
                     EEGEGLAANLLRRYGLTPQALRQQVSKVVGKGAEDGRAETPTNTPELDKYSRDLTKMA
                     REGKLDPVIGRAQEIETTIEVLARRKKNNPVLIGEPGVGKTAIVEGLAQRMVAGEVPE
                     TLRDKRLVELNINAMVAGAKYRGEFEERVQKVLKEVTEHQGEMILFIDEVHTIVGAGQ
                     GGGEGGLDVANVFKPMMARGELNLIGATTLNEYQKYIEKDAALERRFQPVMVPEPTVA
                     QTMMILRGLRDTFEAHHKVSITEDAIIAAAELSDRYITARFLPDKAIDLLDQAAARVK
                     LSATARPVAVQELESELHQLRREQDYMASRKQYDKAAELGKRIEAKETELKQLVEEWE
                     RERASGSAEVKAEHVAQIVSRLTGIPVNELTVEEREKLLHLEQRLHERLVGQDEAVRA
                     VADAVRLSRAGLREGDKPVATFLFLGPTGVGKTELAKALAESIYGDEGALLRIDMSEY
                     GERHAVARLVGAPPGYVGYDEGGQLTEKVRRKPYSVLLLDEIEKAHPDVYNILLQVFD
                     DGRLTDGKGRVVDFTNTIIIATSNLGSDIIQRRLKAGGAAGEEYEKTKSEVMDVLRGH
                     FRPEFINRIDEIIVFHALGKEEIRHIVGLQLDRVARNAASQGVTLTFDQTLIDHFAEE
                     GYKPEFGARELKRLIRSELETALAREMLGGGIGKGDHANARWDDKAERVVFERQEPPA
                     KSAEPEKPDAADVAEAPPGDASKPARKKKSADGES"
     gene            18188..19618
                     /gene="cls"
                     /locus_tag="RHZ76_002037"
     CDS             18188..19618
                     /gene="cls"
                     /locus_tag="RHZ76_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDV2812679.1"
                     /translation="MSDPNGLTWAATLVSLAVAIPTAGHAVIYKRDPRSATLWVLLIA
                     LLPLGGSLLYGLFGINRYQRRARRLFPGADPAVRQDLSPTIPEAVSPPLAGLAHLVGR
                     ATGQSLTGGNRIEPLVDGEQAYPAMLAAIESARYSVALTSYIFDSQGIGAQFVDALRR
                     AHERGVQVRVLIDDVYARWTPRSAYRALQRAGVPAATFNPTLIPARLHAAHLRNHRKL
                     LVIDGETGFTGGLNIFSPYWRPDAPDQACHDLHFRLRGPVIAHLMRCFTDDWCDTTGE
                     RLSKGYWGEPPVTADEQGTSWARGIEAGPDEALDRMRWTFMGALSAAKHSVRIWTPYF
                     VPDQPMIAALSTAALRGVRIDVLTPANGDHPTVQWAARAHYWQVLEHGVRIFERPGPF
                     DHSKLMLVDGAWCCLGSANWDARSLRLNFEFNVEVYDTALSTRLSSLFDAARDASGEI
                     SAKALRARPLAIRLRDGVARLFTPIL"
     gene            19646..21475
                     /gene="ftsH"
                     /locus_tag="RHZ76_002038"
     CDS             19646..21475
                     /gene="ftsH"
                     /locus_tag="RHZ76_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDV2812680.1"
                     /translation="MEKKDQWNIGYWIVAGLLLLTLQNYWQAAKTVEPVPYSEFEKAL
                     AEGRVAEVLVSDRTVTGHLKSPDSRGKTTIVATRVEPDLAERLSKYDVPYARVVESTW
                     LRDVLSWILPAVAFFGVWFFLFRRFAEKQGMGGFLSIGKSRAKVFMEKNTGVTFADVA
                     GVDEAKAELVEIVDFLKNPQEYGRLGARIPKGVLLVGPPGTGKTLLAKAVAGEAAVPF
                     FSISGSEFVEMFVGVGAARVRDLFEQARGQAPAIIFIDELDALGRARGVGGPIGGHDE
                     REQTLNQLLTEMDGFDSSVGLIILAATNRPEILDQALLRAGRFDRQVLVDRPDKKGRL
                     DILKVHVKKVTLAQDIDLEQVAALTTGFSGADLANLVNEAALAATRRKASAVELQDFT
                     AAIERIVAGLEKKNRVLNPKERETVAYHEMGHALVALALPGTDPVHKISIIPRGIGAL
                     GYTLQRPTEDRFLMTRADLEHKIAVLLGGRAAEKLVFGELSTGAADDLARATDIARDM
                     ITRFGMDEGLGYIAFEAQRPRFLDTPELAHGGCRVAESTQARIDQAIRDIVMGVFERA
                     YRILDTNRAVLERCARELLARETLDENDILQLTQGLVLDRNEK"
     gene            21554..22012
                     /gene="hsp20-GI"
                     /locus_tag="RHZ76_002039"
     CDS             21554..22012
                     /gene="hsp20-GI"
                     /locus_tag="RHZ76_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock protein sHSP20-GI"
                     /protein_id="HDV2812681.1"
                     /translation="MSALTPWDPFRELDELQNRLATMLGRTPQRQGARTGNEALTTAD
                     WAPMVDISEDENAFLLKLDLPEVPKDAVRVSAENGVLTISGERKLEKEEQGKKFHRIE
                     RAYGRFVRSFVLPDNVDPTKVTASMKDGVLEVRLVKAEQAKPKQIEISVN"
     gene            22034..22942
                     /gene="yfdX1"
                     /locus_tag="RHZ76_002040"
     CDS             22034..22942
                     /gene="yfdX1"
                     /locus_tag="RHZ76_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat resistance protein YfdX1"
                     /protein_id="HDV2812682.1"
                     /translation="MNIKQPQYIRSALALAVCIGLSGPVLAQSAASPSAAAPSVAPKA
                     AQPQVDDKAAQEAEKKRSELTQDAITALTKTQEALTLLDANKTKEALAALELATGKLE
                     LVLARDAKLALAPVDVRVITHDIHANVESVKKAVKLSRELLGDGEVQKARPIVANLAS
                     EIVIETDNLPMATYPAAIKSAARLVDSGKIDEAKAELARALNTLVVTQVVLPLPVLRA
                     EAAIAKAEKLAETDKRDAKQNEELSTLLSSVRTEIELAQILGYGKKEDFKPIFDQVKS
                     IEQKSAGGKSGNGWFDELKTRIQKLF"
     gene            23045..>23366
                     /gene="yfdX2"
                     /locus_tag="RHZ76_002041"
     CDS             23045..>23366
                     /gene="yfdX2"
                     /locus_tag="RHZ76_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat resistance protein YfdX2"
                     /protein_id="HDV2812683.1"
                     /translation="MNEQTSNPNAINEGTNEQAAVSSLPARPEPKPEVVTEVQPEVQK
                     ETDSQAADKRKQVLDEAVSALALTKSALAALDEKESARALATLAEVTGKLELIVAREP
                     TLALA"
BASE COUNT         5161 a         6472 c         7056 g         4677 t
ORIGIN      
        1 cgtcatttaa aatcctgttt ccgctccatg gtggtaactc gtatgagaat taaacatcta
       61 atatttttac ctgttccctt catttccttt tatttaaacg cggcgagcct ggacgaaatc
      121 aatcaaaagc tggatgaagg ttaccgttac gcctgtaacg caactcagac gggtaatgca
      181 gaagccgcct ccctcgcgct ggagaactac agaacaaaat tcatcaactc agcatcacga
      241 tctggtttca gcgagataat gaatgtagaa ataaacaaag ttgtcgcgga atttttcaga
      301 aagggcatga tccaggggaa caccagcact ggggcctgtg agactccagc ggagctgctg
      361 ctccagacct ctcaggcggt ttattcccgc cagtcaaagt cagcactggc gtcagccctc
      421 gatggtgtcc gattcactga atatgcggca caggcctacc cgatttttta ttcaatccaa
      481 agtacaggta tcagtgagga tgaagtaatt gaacagatta ataaaatagg aaacaatgca
      541 ataattaata accaaaatgc aattgatgtc agtaatgcga caaatgcaat gaaagaagta
      601 gcgaaagtcc ttttttcagc ccgaagtgac ccggagctca tgaaatttat gatggattat
      661 attaataacc acaaactgca ttgaaggtaa ttgcggtgta taaaacaaag ccattttcgg
      721 ccaaaaaatg agtcagctga agaaacgctc gttaattccg aatccgtcca gattaatatg
      781 gcacactgct ggtggaggtt ttggcaatcg gaaaagtaac ttttcacccg tgtctaatca
      841 tcatcgtttt atcaatccgc tcagttacgg tattaaaatc tcacgaaaaa tagtcttctt
      901 atcgaagtgt catgctgtct tcgcccgata tagtgaaggt atgtattcag cggggccact
      961 gacatgcgaa aaagctatac ctatgggatt cctttcggac tccatcgcga taccggacgg
     1021 tttctggaca tcacggaagt cagcagagga agcgcatgca actgcatctg tcctgggtgt
     1081 cgtacggatt tgatagccag acagggagag gtcaaactct ggcactatag ccacagtacc
     1141 gatctcctcg gtgattgtga tggcctgatg gaggcgattc ggggaaaaat tattgaagtt
     1201 attcaggaaa aacaacttct aggattcccg catcttctgg ccggttacga tggaggcacg
     1261 gtcccacttg atgaagtcag cggcagcgga agcatgttcg gcgcaacggc agatctgttt
     1321 gtgaaggtga acgatctctg catagctgtg tttctggaca ttgaccgcag tgtggccgga
     1381 aaactgacgt tcgaccatct ccatgcctcg gagatggtag ctgcattgcg tattgatctg
     1441 ccggatatag aatacgaaat cagtcaggtt cagcttggta gacgagatgg tacctacagt
     1501 gaatgtatcg agaaaatcat cattgatgcg acggagtcac gcgaatggtt gtatcacccc
     1561 atgatgcagg aactggggac cgagcccctg aaggtatatg agggccgcga gccagcggaa
     1621 gcaggaccac ttctacaacg cctttccgac agcaccctga agctgccgga caacttaccc
     1681 cagagcatga atgtgctgat aagaatgcag caggccacca ttgtgtgttt gtgtcggctc
     1741 accgtcagag cttcagatat cgcccagacc gacgaattcc cgctgtttat gcgttacttc
     1801 cggctgcatt gcctcgacac cagtgagcat gtgaattacg agaaattgct taactggcag
     1861 gacatgatta caaaatcaaa ggaagggcag ttactaacgg tggaggaaag ggattatctc
     1921 caggaaatcg tccgtattgg tctctataac caccggctta aaagtgtctg cctgacggcc
     1981 tgcgaagaca atagggtggg agaatctgaa ggaccaaaaa attactcatt gctctgaaga
     2041 gggatgggaa attatggggg ggcatggaag cgtgaagaga ataaacctgt tcgccttaaa
     2101 cagaaacagg ctgttcattt cttacatgtg aacagagtac ctatcatgat cagaccggta
     2161 atataaattg gcacagccat caaaaccaat cccagtggtt cttctttggt aaaaacatcc
     2221 tgaataagtc cgtcttcgct taatgctaat ggacgcatac attagaatta aggcgtaagg
     2281 tctaataact agatcaggag tttcggattt ttgcgcggta ggtgtgtttg accctttcaa
     2341 ttgtcgcaag agttgaattc cagtagcgtt cagcgtcaga acgataaaga tcagcgatag
     2401 cctctatggt tttgggggaa caggttgcac tgcgatgcac acggcctgcc tgaccaaaga
     2461 catagacaag aatggactgg ctaccagacc ggccgaacag ggagttgcaa agccgcttcg
     2521 ctgcgagcgg tgtcagatcg gggacgtctt cctgagcgtt ctcgggaaca acgaattcca
     2581 gaatgaagcg aagcatctcc gatgtcttga aacgctttac cgccgcagac aaacgggcgg
     2641 cacgcagggc agtgtcccag tggtcgctgc tgaaattgcg gagctccgcg aggtaatcct
     2701 cttccaggct ttcatcagaa taataaatgc tttcgtaaaa gcgctctgcg ggtgattgcg
     2761 tcttttccat tggagaaacc attttgccta atgtatgcat acgttagcat gtagtgaaat
     2821 taaaacaagg taaagacaaa gaaccgcatt caggcgtttc ccggagtaag cagatgggct
     2881 gacaattcgg ctgcggtaat atcaaactca aggtgtttga tgagctctgt taattgagcg
     2941 ttaaatgcct cccgctccag tccggattgg atgagaaaga tcagggcctt tttcagaacg
     3001 ggcggagtac tcgcaatgtc gaggagaatg ttgccctcaa taacatgatc gcaattttca
     3061 aaaaggtggg cgtaactcat tacgtgtatt ccatttatca ttttcaaatc gtgataaaaa
     3121 ttagattacc agaacgtcgg aagcaaaaaa aagcattaag cagaaatgta tggtatccat
     3181 ccttcctcca gctacccttg cgccctgata attttatcct gggggagggg gcattgatca
     3241 ttacataccc gatcagaaaa gccgggaacc agaatgtcac ccggatatcg aagatgcaac
     3301 gattcaatat tagtgtattt agcgtctcct ggcggcaaag tcggtcggcc ggaactggta
     3361 aactgtattc cacctcgccc gggctggcag gcgactgacg tcttgtcggt tcatagccgg
     3421 tggtcgcgtc tgacaataca ggcccctaca gtgtaaggat tgtgatggac gataaagagc
     3481 agtttacgag tctcgtggca aagcatgcct ccagactcac cgaagagcag ctggccgggt
     3541 atgatgactg ttcccagtat ggtgaatgcg tctcgccttc atacgaagta ttccgggggt
     3601 atcgtacccg ccacaccctg gatgaatttc tggagctggc catatcgctg aatgccatcc
     3661 acccggatga atatttaacg gatatgctgc tgaagcctca tgaggtgatc ggtgctctgg
     3721 ccgatgaagg cgaccagttg aacaacgcca ccccggttta tatcttcccg gataccggcg
     3781 tctatgcagc ggccgtcagt gaaacccgtg tgctcgatgc ctggctttgt tggccatgct
     3841 acccggcgaa ctggtaatgc gtccgttgga attgcaccgg caggatacga atttaaccga
     3901 ccccgtggcg ggagggttat cacaattgca tgtcctcatc cctaaagtcc cctgccagac
     3961 ccatacgtgc tgtcaggctt cgcctgattt gagatcagtg caaatacttc agccgataac
     4021 tttttgtttt ctttttattt tctccctgtt tcctcaaaat tcgcgtgcgg cctggtcagg
     4081 gcacgattgt cacatagggt gtattgaagc gggcaggatg gtcgggtggg cacggcctgg
     4141 gtttgagtcg gacactatgt gggatgagga catgcaaaat attcgcttta tctgtgaaaa
     4201 ctgttgtcta cgggcagtca ccagttcaag ttacaccatc cacgttagct tttaataaga
     4261 gtggttacga tgttcagtac tgagaaaaaa tcagagcatt taggtcagat catagtgatt
     4321 gatcccgcgc ttcttgggca gggtatagag gttggcccgg tcgtagtgtt tgatttgggg
     4381 cacgccagca aaggggttgc ggacttgatg cgcgatgctg aaaagcttga tggtttatcc
     4441 ccaacgacaa tagcaaacag actttcacga tgaccgaaca aggtcgattc gggcaaggcg
     4501 gcggaggggc acatctccca atgataatga ggttatgcaa cctgttagtc accggcttgc
     4561 ctgatgttat gattgacatc tcttttgacc ggtctgtagt tgagcattaa ttcgtgagga
     4621 atacggtatc agcggataaa agtagcatgt ttgctatcgc ctccagctgt gaaaaccctc
     4681 ggtgggtgca agaagttgag agccgtgggg ggatgacccg taaggaatgg gctgactttg
     4741 tggatcgaaa ttttaaccct cctaaataga gtaaattccg attggcattg ttgcaggaat
     4801 tatggtgccg cagcttattg ggtgtttgtc aattgccggg aagtattcgc tggcatatca
     4861 cttaaacggg agtagtaagt gagagaacct cgtccacgcc atacattaca ggtagtctgt
     4921 gtcccgtctc tgaatataca ggatctgggg ctaaccagtt ttcatagctg gctcttatca
     4981 aaaggctatg acacgaccaa tgccaggaac aaccgtactt gctgggcaag ggaaggtggc
     5041 tggcacctga agcgatgcag acatctggca acgggcgaag atcatttctg gtttatcgca
     5101 ttcaacggta ccggcggcga catttttcct gtgaaaaccc agcaggacta ccgtgctgcc
     5161 taccgcaagc ttgaagcgta tggatatgca ccggccgtta ttgaacaatt gaacacaggc
     5221 gttgcctata atcttgctta ttcacggaca cctgtgggat gcaaataaag tatcatccgt
     5281 ataaataaag tttcattctg agcctgcatc cgctatgttg aggtcatcga cacagcgggg
     5341 gcaggctttc tcatgtctgg aacattcaag gggctgaccc aggcccagat cgatagaagg
     5401 ctcaaggacg ggcgtggtca aggccaggga tcagactaca agccgttcat ctacacaagg
     5461 gacgtgtcct cgctgggtcg ctcccaccgc ctgcctggca gcaagacccg gcgtttacac
     5521 catcttctat ccgatcttga actggccatc ttcctgacct tggatcggtc tccccatgtc
     5581 accgatatcc gtgaacagtt tccgatgcgg gtcgaagata cggtgcgcat cgccgaggag
     5641 cttggcctgc cccacggttg ttacaagggt accccgcagg tactgaccag cgactttctg
     5701 gtggatttcg aagatcctca gcgcccgagc atcgcgattc aggccaagta cagtaccgac
     5761 ctgcagaagc ctgaagttat cgagaggctt gagcttgaaa ggcgctactg gcaggaaaaa
     5821 ggtattccct gggtcatcgt tacggagcgc gaggtatcca aggtggcatt tgccaatatc
     5881 caatggctct atcctgccca tgctgaaggc gacatcgctc aggacgatct cgcccattac
     5941 cagcagctgt ttctgctcga gtttcagcgt cacccagacc ggaagctgac cgctattgcc
     6001 caaggactgg atacctccgg ccagctggag gccggtcagg cgctctattg gctacgccag
     6061 ttgctggccc ggcactgttt ccttttcgat cttgacactc cttaccggga gctgaagccg
     6121 aaagacctgg ttgccaactc tcatcaaatg catcaggagc tgagcagtgt tgcccgttaa
     6181 tcaggtgttc cggatggggg aactgcgaaa gcgcctgctt tggtccggta ccgagcaggc
     6241 aatctggatc gatatcgatt cggacacggc gttgcccgaa ccgatatcga ttgtagagct
     6301 ggagcgtctg atcatggagc gggagctgga gagcattgcc gatcccttcg aggaaacagt
     6361 cctacgggag gttgaggaag gttcccttga tcagcagaag cgtgatgaag cttggggaat
     6421 gctggcggat tttgtgcaca acccccagct tttcgtgcgg cgccccagag ggctcattgt
     6481 cagaggcatc atggagcgcc atggtgtcac caatcagacg gtgtataggt tactgaggcg
     6541 ctactggcag cgaggcatgt gcaggaatgc cctcttgccg gactatgtca actcgggcgc
     6601 ccgtggcaag cgcagaaagc cgaaccaggc caagctgggc aggcccagag tggtgatgga
     6661 ggggaaaggg agcaatgtca cgccggacat cgagcgcatt tttcgccggg tcatcgagga
     6721 acgtttgctc aaggaaaaac atccgtccat tcctgatgcc tacgccgccg ggctgaatct
     6781 gctccgtgct gttctgacgg agctgccgac ctccgaactg cctacattgg ggcagttccg
     6841 ttacttctat gggcgcgaat atcacttcac cgacaccttg ccatgcaggg tgtccgcagt
     6901 ggattttgcc aaggacttcc gaccgctcag cagtacatcg acgaccgagg cactggggcc
     6961 aggataccgc taccagatcg atgccaccat cgccgatatc tatctggttt cagaacatga
     7021 tcgcagcctt atcgtcggca ggcctgtcgt ttacatggtg atcgacgtgt tcagccgcat
     7081 ggtcgttggt atgtacgtcg gattcgaggg accttcctgg gtcagtgcca tggtggcatt
     7141 ggccaacacg gtcgccgaca aggttgagta ttgccgccag tatagcgtgg agatcgatgc
     7201 cagcgactgg ccggtcaaag gcttgccgga tgtggtgctg gccgataagg gcgaactgaa
     7261 tggcaccaag gtggaagcat tttcccaagc cttcggcgtt cgcatcgaga atgcaccggc
     7321 caggcgagga gatgccaagg ggatcgtcga gcgttatttc cggacggtgc aggagcgttt
     7381 caagccctat gccagcggtg tggtcgagga caccaccagc cggaagcggg gtggccacga
     7441 ctacagactt gatgccagtc tgaccttgcc cgagttcacc cagatcatca ttgccggcat
     7501 cctctaccac aacaactttc ataccttgag caaatacgac agaacctctg gtatgccggg
     7561 cgatctgcca gccatcccgg tcatgctgtg gaactggggc ttggccaact tgaccggccg
     7621 gttacgtact gcgcccgagg agctggtgcg gataaatctc ttgccccatg agtcggccac
     7681 ggtttcggag ctgggaatca gactgttcgg ctgcttctac acctgccagg aagcaatcag
     7741 ggagggttgg tttcaccgag gccaggggcg ccggccgaca ggggtaacgg tcgcatacga
     7801 tccgcgtagc gccgaccaca tctacctgcg gccgtccaat agtctcaagg actactgggt
     7861 ttgcgatctt gccgaccgca gtcgacgctt cagggggatg accttctggg atgtctggct
     7921 gctgtccaga gaggaacggc gtagcgatac caacgcggcg gcggaagctt tggtagagag
     7981 aggcaatctt ctgaagcaga tcgagtccat cgttgcccag gcagaggatg ccagtccgct
     8041 caaaacgggt atgaccacga aggatctggg aacccagatc cgcgagaaca agcagcagga
     8101 aaagcgccag gaacgccaga aaacagcatt caaaccggat aaagcacagc aggaaaagcc
     8161 agccgaggtg atcccactgc ggggcgagaa gcaggaagat tatgccttcc cagatctcag
     8221 tgacctgata ttcaaggagg aagaggatga ctgaagaatt ccagcgcgga gtcattcccc
     8281 tggcgcgtta ccaggagcag cagcttcccg agtatcagga caatccgctc atcagcgcat
     8341 tgcctccgat tcctgatctg caggaggtgg tcgatctatt gcagcagctt cccggtttcg
     8401 agccgcagga ggcgcttctg gatggtcggc tcagggcaca tgcgattgcc cgcctgttgc
     8461 acggcttctt ccagccgctg agccatcact tggagctgga gagcaagatc agcctgatga
     8521 tccgtcaagg gtacatcggt cggaatccgg ccagcggtgc ctggtatgcg cacctgcaga
     8581 atggctatcg ccgtgtcgag gaggaggatc tggatgcggc ggtgtaccag agtgtctcct
     8641 ctacggccaa cagcctgtcg ctgttcggct gctccggttg cggcaagacg cgcacgctgg
     8701 agcggattct gggcatgtac ccccaagctc tccatcaccc tgattacgac atcacccaac
     8761 tggtctacct gaagatcgac tgcccaattg acggtgactt ggatgagctg tgcctgagct
     8821 tcttcaacga agtagacaag atcctgggta cccactacag ccgtagccac ggacgcaaga
     8881 agctgggtac caaacgtctg ctggcatcca tgtgccagct ggccaatctg cacacgctcg
     8941 gagtgctgat catcgacgag gttcagaacc tcaacgaggc acgctccggt ggtgccgaaa
     9001 aaatgcacaa tttcttcgtg tcgctggtga acaccatcgg tgttccgatc attcaggtcg
     9061 gcacccaccg ggccagcaag ttcttccagc gcacgttccg ggctgctcgg cgggtctccg
     9121 ggttgggcag catccgctgg gataggttgc ctcgggacga gcattggaag cgtctgttga
     9181 agcggctttg gaagtatcag tggctgcgaa atgcggcgcc gctggatgat gaactggagg
     9241 ctacgatgta cgacctcacc caaggggtca tggacatcgt catcaagctg ttcgcgctgg
     9301 cgcagatccg ggcgatagtg acaggtgtcg agtgcattaa gccgctgttg ctgcgcaggg
     9361 ttttcgagga cgagttcaaa ccggtccatc cgatgctggc ggccttgcgg agcggtaggc
     9421 ccgagctgat cgccaaatat gacgacctgc tgatgccaga gatcgaggga cggttgctga
     9481 ccctgactcg ctctctggat agcgtccccc aacctaaacc accggcccct ccggctgatg
     9541 acaaggcaaa gcggttggcc gccctgctcg aaggtatgga aatcccgagg gacatcgcca
     9601 ttcccatggc cgatgaattg ttggcggaga atccggacat cccgctggcg gccctgattc
     9661 acaaggccac ggcctacctc gcgcaggacg ctcccaagag gcccggcatt tccagagtta
     9721 agcggacgga gtgggggtgc ctatccgagg aggatctgcg ccgacgttat gccgatgggc
     9781 ctgaaagcga ggcctatgac agattcaagc aggctggctt gatcctcgat ctacggcctc
     9841 tgctgagagc gagctgacat gtacttcccc aggctctacc cggacgaact gctttacagc
     9901 ggtattgccc ggtgccgggt acatctgggg ataggcagcc acaagacctt gctgcacatg
     9961 cttttcggtg acagcaaggt tgccgcgatc accgatctgc ccacacacct gactgcgttg
    10021 gcgaacaaca cgggcttgga tgcggaaggc atgatcctga atcacacgct gttcccgctc
    10081 tatgcccctt tcattccgcc ggagcgccgt accgatcttt ttcaggccat gcttgctccc
    10141 gaccggccga cgattggtct gtctggggcc tcgacggcct tggtcaagtg gcccgattgg
    10201 ctgcgttact gcccgatctg ctttgaagat atggctgcgc gctacggtga accctactgg
    10261 cggcgtagct ggcagattca agggatagat gcctgtcccg agcatggctg tcagctcctg
    10321 aactcaccta tcccgttccg tcgagcgcag cggcacgagt ttcatacagc ctctccactg
    10381 tttctgcccc gtgatcttcg cgtgagtccc gtgggcgaag aggctatcag gctcgccaag
    10441 gctgctatgc agctgctggc gctcgaagag gtgcaatcgc ctggctacgg acgctggacc
    10501 aacctgtacc ggtacctggc tacagagtgc ggcgccaggc gaggccggca ggtcagggct
    10561 gaagtcatct gggagaagat cctggcatcc catcaaaggg attggcttgc tgcgaacggt
    10621 ctgctgacat ccggagagcc tccaccttgg ctgttggcga tgttcaggaa gcatcgcaag
    10681 ggattcagtg cgctgcagca tctgatcgtc tggaccagcc tgcgccctgg ccagcatgct
    10741 ggcgagctga tcagcgaggc gaagactcgc caggtcgacc ttgcctccga gtggtcagtg
    10801 cagctgctcc ctgctgagtt tgagcaaaag cagcagtacc gagccatttg gctgcaagca
    10861 ctggctaatc acggtggagc caaggcagcc aggcaagacg gagcaggggc atgttacgcc
    10921 tggctctatc gtcatgaccg acactggctg atggttgcga accaggtcag gcagcgccga
    10981 cagggcaaca acagccacat cgactggcga gcgcgtgaca taaggttggt gcgcctgctg
    11041 atccggattg gtagaggtag cgaagaagac ctgggccttc cgcgcaggag tcgcaactgg
    11101 ttcctgcagc agctacccca tcgggcatca gtcgagcacc gtctcgatca attgcctctt
    11161 tgctgcacct tcctggatcg ctatgccgag tccgtcggcg agtatcagat ccgtcgcctg
    11221 actgcagcga tgctgaagga tgtacagaca ggcataacgt ctaggcgctg ggagctggaa
    11281 aagcgttgtg gattggaaaa aagcagggtg gcaccactga ccactgcgtt cattcgattg
    11341 ataggaagat ggattgaata ggacagtatt tcgtcagatc ccgaacggat ccaggttgct
    11401 ggccgttgga aattttttca gggccaagac tccggcggcc cagtggcagt tggcggctct
    11461 ctttgagcgt aacggtcagc tgcagctgca gcggttcgca caggagatgt cgtgtgtctt
    11521 ggccgttggt cgcgagtttc ttggcgaaga aggggctcct tatcgtccca gtggtttcaa
    11581 gaagacggtg gtgcttcctg caatagatac ctggcgggaa aggcagttgg gtgagtgccc
    11641 acgtctggcc agaaggcttg ctgcaaatcc tgagatatct ggacagcgat gctttgtgtt
    11701 cgaggtcgat ggtctgaccg tctggctgcc gaaattcgag ctggcacgaa aaatgttctt
    11761 ccacgcagcc ttcatcgttc gtgcagcctt cgcaccgaat ggcttggaca tggccttcac
    11821 gatctacaga gagggcgatg ttgttcatat ccatacgccc ccgaagacgg gggccccaag
    11881 tcagcttctc aaaatcaagg gatacagaga ccatttcagt tggctgctgc tgaatcagga
    11941 tgtgaggcga tccttcgagt caatctggca aagcctcaat caaaggcaag agcgggtact
    12001 gcaagagtcg gcgtatgcca ggtggcagtt cgactttcag gcacccactt ccctcgcggg
    12061 taccaccatg gatgtgcgag gcccctttga tccggaaagc aacgaattgc tggtctggga
    12121 gatcgaaaag cttcaggggc tccggtttag ccatcgcggt gatatttttt tccatcaccc
    12181 tgctctcaag ttacccgtca ggggagagaa cacaggctgt gtatccgtgc catcgggagc
    12241 gaaagagttg gaggtggatg ctgacgaaga gccgactgct gccaaggagc agaggttgat
    12301 tcatctgccc gttgagggag tcacgtttga tgctcagctg cgcaccaaga tcgcctacaa
    12361 cggggagcgt gccaacggtc atggcaagaa ggtcgacgac gagcaatgcc cgagcagaga
    12421 aagccaactc ttcggcgtgg ctgatcctgt gagcgagggc agcatagcgt caggagaatt
    12481 ccaacaattg ggtgagggag agggtgatgg ggacgagcat gaacggcttg cagatcgctt
    12541 ctcgctgctc cgagagattc ttgaacaaat cacccaagag tctggagttg cgctggtagc
    12601 actggaagtg aggcctctgc cgcccgttcc acgttgcagc taccacatga ttgatcacga
    12661 aaaatcacgc tgctatctgc tggtaaggtt caagctgcaa aacggcgatc aacgctacct
    12721 tctagaaata gatacctcag acaaccggaa gaccatgtca acgcggatca tgggcttcaa
    12781 agcaggtgtt gaggcaggaa aatgcataga caggatcttg aggggaacag tgaaaggctc
    12841 gttgcgttgg ccaggcacca tggcgaagta ctgtgagcct ttgcactctg tgcatcaccc
    12901 gaaagaaagc tcatctgggg ccaaccatgc ccgggtgttc gactggaagc agcggatacg
    12961 cgcggcccta ggctgattgc agaatacacc gcagttcggc tgagatacat cttgtcgcgc
    13021 actgcgtgca gcctccccga aactgcccgg agccgtgcca aggcgatata ggccccttgg
    13081 gagtcggcaa gacgcatctg gccatcgcac tgcgctatcg ggcggcgcag tgcggcatca
    13141 agacccgctt catcactgcg gccgaccttg gtgctgcaac tgggcgcgga ccagcgtaac
    13201 cgtccgggca tcccggctgg cttgaccccg ccaagctaag agatggtggc agttcgtgga
    13261 tgccgtttcc cgggtgcacg cgatccgaga ccacgcgatc aagcaggaaa acaagcacct
    13321 gggcttgcct gactgaccgc aggccggcga cgacaaggtg gaaaagttcg gcgtgtggct
    13381 ctggcggcaa cgatcccgcg aagggcggac ggtgcttgag ctgttgaacg taagcgtgcg
    13441 ggcagaccgt cttgcgcgac tatgccggat gccacctatg cggcagcagc tctgcaatct
    13501 cactcgcccg ctgcgtcggc aaccgtgtca gcacatcctt gagataggca tacggatcgt
    13561 gcccattatt acgggccgac tggatcaaac tcatgatcgc agcagcgcac ttgagcatgc
    13621 gatcccgcgc cgagcatttg agaaggcaac gggatgaacg ctggctggat agccgtggtt
    13681 ttctgggtat gcagccgaat ccatttgtgg acgaggttgg cattgaggct gtagcccaag
    13741 gcgatgtttg cgatggaggc accgggttca gaacactcct gaatgacctg ggccttgaaa
    13801 gatttgctgt aggtacggcg ctgagggggc atggaaaatc cgcttgatgg gccatggcct
    13861 tcggcggcgg tgtcgggaag gtgtgttggc aggacggata ctgttgaact acgttgtctg
    13921 gggcaacgga ggtttttcgg cccgactctg gaacgcgatc cgcagcgacg actgggcaat
    13981 tccgcacatt ggcctgagca gcctcgggga aatcgtcggc tgggcgcgcc cagacgagtt
    14041 tccgccaagg aacatgcgca cgagcaaggg gctgcgggcg ctcggttaca acgtgaggat
    14101 cggtgtttga tctgcggccg tgggcggcgc ccgcaaggtc ccgatcaacc caccgtcccc
    14161 tgcgccggac cggcccgccc tggcataccg tccttgaaga tttgagtcac atcaatcggc
    14221 cagcagatcg ctggatgttg actcttgact tttggcatat taggcgcaat atcttgcaaa
    14281 gtcttgcaag aacttcctca ggcgaggccg cgtgacgacg gaacttgtgg gggtggtatg
    14341 acagcgcgcc agcaggcgat cttcacactc gacgagttgg ctacctactt aaaggtcggc
    14401 aagcggacgc tctaccggct cgccgcgcac ggggagattc cggccttcaa ggtcggcggg
    14461 acgtggcggt ttcgtcaaag tgaaatcgat cgatggatca atgatcagat ccaagcaggc
    14521 agaaagaagg aggtgatacg tacagatgag cagccgaagt cagcgaaaca tcccgtgttg
    14581 cctgggcgcg ctatccatag ccgcaggcaa acggagtgag cgagagtctt caatttttgt
    14641 tctggaggtg tgacatggac attgatttca agaagttggc tccctggaac tggttcaaga
    14701 aggagcagga agaacaacag agcactgcct cgctgccggt gcagcgcaat gacctgccgg
    14761 tagcgggtgg gcccgtcagt cccatcctgc aattgcaccg cgagatcgac cgcctcttcg
    14821 acgacacgtt tcgaggcttc ggctttccgg cgctggccat gccacgctgg ccgtcggact
    14881 ggccgggcct gctgaagccg gcgctggaca tccaggagac cgacaagcag tacaagattt
    14941 ctttggaggt tcccggcgtc gaggaaaaag acatccatat cacgctcgac aacgacgtgc
    15001 tgctggttcg cggtgaaaag cgtcaggagc aggaaagcaa agacggcggt ttccaccggg
    15061 tggagcgctc ctacggcagc tttcagcgcg ctctgaacct gccgaccgat gccaaccagg
    15121 acacgatcaa ggccgctttc aagaacggcg tactcacgat cacaatggaa aagcgcgagg
    15181 ccagtgcgcc caagcagggc cgctcgatcc cgatcaacgg ctgacgccga tcggcttgtt
    15241 ctctacagaa aaaccaagtg aatggcgagg cgccgtgacc ttcggcgcct cgtcaaatca
    15301 gtcctctcaa ggagcatgag catggccaga aaacaatgcc aggtctgcgg ccagcccgcc
    15361 acggtgcggg tggaagccaa tctcaatggt cgccacagca ccatgctgtt gtgtgacgat
    15421 cactatcgcc aactggcgcg ccagcaaaag cgcaccgtct cgccgctgga agccttgttt
    15481 ggctcgcgca gcggcttgtt cgaagacttc ctcggcagcg acttcttccg catcggtgac
    15541 gattcgccgt ccatggcggc cgataccgac gaggtggtcg atgcctcgtt cggcgaaccc
    15601 gccccggccg gtacgggcac tgcgcgccgt cgcggcagtg ggctcgccag ccgtatcagc
    15661 gaacagtccg aggccctatt gcaggaggcc gcccgacacg ctgcagagtt cgggcgcgcc
    15721 gaagtcgata ccgaacacct gctgctggcg ctatccgaca gcgacgtggt gaagaccatc
    15781 ctgggacagt tcaagatcaa ggtcgatgac ctcaagcgac agatcgaatc cgaagccaag
    15841 cgcggcgaga agccgttcga gggcgaagtc ggcgtgtcgc cccgggtcaa ggacgcgctc
    15901 agccgcgctt tcgtggcctc caacgaactc ggccactctt atgtcgggcc ggagcatttc
    15961 ctgatcgggc tcgctgagga aggcgaaggt ttggcggcca acctgctgcg ccgttacggc
    16021 ctcacgccgc aagcgctgcg ccagcaggta agcaaggtgg tcggcaaagg ggccgaggat
    16081 ggccgcgccg agacgccgac caacacgccc gagctggata aatactcgcg cgacctcacc
    16141 aagatggcgc gcgagggcaa actcgatcca gtcatcggcc gtgcgcagga gatcgagacc
    16201 accatcgagg tgctggcccg gcgcaagaaa aacaacccgg tgttgatcgg tgagcccggc
    16261 gtcggcaaga ccgccattgt cgaagggctg gcgcagcgca tggtcgccgg tgaagtgccc
    16321 gagacgctgc gcgacaagcg tctggtcgag ctcaacatca acgccatggt ggcaggcgcc
    16381 aagtatcgcg gcgagttcga ggagcgcgtg cagaaggtgc tcaaggaagt gaccgagcac
    16441 cagggcgaga tgattctctt catcgatgaa gtgcacacca tcgtcggtgc aggccagggt
    16501 ggcggcgaag gcgggctgga cgtggccaac gtgttcaagc cgatgatggc gcgcggcgaa
    16561 ctgaacctga tcggcgccac cacgctcaac gagtatcaga agtacatcga gaaggacgcc
    16621 gcgctggagc gtcgtttcca gccggtgatg gtgcccgagc cgacggtagc gcagaccatg
    16681 atgatcctgc gcggcctgcg cgacaccttc gaggcgcacc acaaggtcag catcaccgag
    16741 gatgcgatca tcgccgccgc cgagttgtcg gaccgctaca tcaccgcgcg ctttttgcct
    16801 gacaaggcca tcgacctgct tgaccaggcg gccgcacgcg tgaagctgtc ggccacggct
    16861 cgcccggtgg cggtgcagga gctggagtcc gaactgcacc agttgcggcg tgagcaggac
    16921 tacatggcct cgcgcaagca gtacgacaag gccgccgagc tgggcaagcg catcgaggcc
    16981 aaggagactg aactcaagca gctcgtcgag gaatgggaac gcgagcgcgc ctcgggcagc
    17041 gccgaagtca aggccgagca tgtcgcgcag atcgtctcgc gcctgaccgg cattccggtc
    17101 aacgagctga cggtggaaga acgcgagaag ctgctgcatc tggagcagcg gctgcatgag
    17161 cgcctggtgg gccaggacga agcggtgcgc gcggtggccg atgccgtgcg gttgtcgcgc
    17221 gcgggcctgc gcgaaggcga caagccggtg gcgactttcc tgttccttgg gccgaccggc
    17281 gtgggcaaga ccgagctcgc caaggcgctg gccgagtcca tctatggcga tgaaggtgcg
    17341 ctgctgcgca tcgacatgtc cgagtacggg gaacgccatg ccgtggcacg cctggtgggc
    17401 gcgcctccgg gttatgtggg ctatgacgag ggcggccagc tcaccgagaa ggtgcgtcgc
    17461 aagccctaca gcgtgttgct gctcgatgag atcgaaaaag cgcaccccga cgtctacaac
    17521 atcctgctgc aggtgttcga cgacgggcgg ctcaccgacg gcaagggccg ggtggtggat
    17581 ttcaccaata ccatcatcat cgccacctcg aacctgggct cggacatcat ccagcgtcgg
    17641 ctgaaggccg gtggcgccgc cggcgaggaa tacgagaaga ccaagtccga ggtgatggac
    17701 gtgctgcgcg gacacttccg ccccgagttc atcaaccgca tcgacgagat catcgtcttc
    17761 catgcgctgg gcaaggagga gatccgccat atcgtcggcc tgcagcttga tcgtgtggcc
    17821 cgcaacgccg ccagccaggg cgtgacgctg accttcgatc agaccttgat cgaccacttc
    17881 gcggaggaag gctacaagcc cgagttcggc gcgcgtgagc tcaagcggct gatccgcagc
    17941 gagctggaaa ccgcgctggc gcgcgagatg ctgggtggcg gtatcggcaa gggtgatcac
    18001 gccaacgccc gctgggacga caaggccgaa cgggtggtct tcgagcgcca ggagccaccc
    18061 gcgaagtcgg ctgagcctga aaagcccgat gccgcggacg tggccgaggc accgcctggc
    18121 gacgcgagca agcctgcgcg caagaagaag tcagcggatg gcgaatcttg agcgatggga
    18181 ggctgtcgtg tccgacccca atgggctgac atgggcagcc accctcgtgt cgctggcggt
    18241 cgctatcccc acagcggggc atgcggtcat ctacaagcgt gaccctcgat cggccacgct
    18301 gtgggtactg ctgatcgcct tgctgccgct gggcggttcg ctgctgtatg ggctgttcgg
    18361 catcaaccgt taccagcggc gggcgcggcg gctcttcccg ggggcagatc ctgctgttcg
    18421 gcaggatctc tcgcccacga tacccgaggc tgtatcgccg ccacttgccg gtctggccca
    18481 cctggtggga cgcgccaccg gccagtcgct gaccggcggc aaccgcatcg agccgcttgt
    18541 cgatggcgag caggcctacc cggccatgct cgccgccatc gaatcggcgc ggtacagcgt
    18601 cgccttgacc tcgtacatct tcgacagcca aggtatcggg gcgcagttcg tcgatgcgct
    18661 gcgccgggct catgagcgcg gcgtgcaagt gcgggtgctg atcgacgacg tgtatgcccg
    18721 ctggacgccc cgcagcgcct accgcgcctt gcagcgcgcc ggcgttccgg cggcgacgtt
    18781 caacccgaca ttgattcccg cgcgcctgca tgccgcgcat ctgcgcaatc accgcaagct
    18841 gctggtgatc gatggcgaga cgggtttcac cggcggattg aacatcttca gcccgtactg
    18901 gcggcccgat gcgccggacc aggcctgtca cgatttgcac tttcgtctgc gcgggccggt
    18961 gattgcgcat ctgatgcggt gcttcaccga tgattggtgc gataccacgg gcgagcggct
    19021 cagcaagggt tactggggcg aaccgcccgt aacggctgat gagcaaggaa cctcttgggc
    19081 gcgcggcatc gaggccggtc ccgatgaggc cctggaccgg atgcgctgga ccttcatggg
    19141 cgctttgagc gccgcgaagc attcggtgcg catctggaca ccttacttcg tccccgatca
    19201 gccgatgatc gcggcgctaa gcacggccgc gttgcgcggc gtgcgtatcg acgtgctgac
    19261 gcccgcaaac ggcgaccatc ccacggtgca atgggccgcg cgcgcccact actggcaggt
    19321 gctggagcac ggtgtgcgca tcttcgaacg gcctggcccg ttcgaccaca gcaagctgat
    19381 gctggtagac ggagcgtggt gctgcctggg ttcggccaat tgggatgcac gcagtttgcg
    19441 cctcaacttc gagttcaatg tggaggtgta cgacaccgcg ttgtctacgc ggctgtcatc
    19501 cctttttgat gccgctcgtg atgcgtcggg cgagatatcg gcgaaggcgt tgcgcgcccg
    19561 cccgctggcc atccgcttgc gcgacggggt ggcccgtctg ttcaccccca ttctctagcg
    19621 acacgacttg cgaggaacat tgcccatgga aaagaaagat caatggaaca taggctactg
    19681 gatcgtcgcc ggcctgttgc tgctgacgct gcagaactac tggcaggcgg ccaagaccgt
    19741 cgagcccgtg ccctacagcg aattcgagaa ggcgctggcc gaggggcgcg tcgccgaagt
    19801 gctggtgtcg gaccgcacgg tcaccgggca cctgaaatcg ccggacagcc ggggcaagac
    19861 caccatcgtg gccacccgtg tcgaacccga cctggccgag cggctgtcca agtacgacgt
    19921 gccctatgcg cgggtggtgg aaagcacctg gctgcgtgat gtgctctcct ggattctgcc
    19981 ggcggtggcc ttcttcggcg tctggttctt cctgttccgc cgcttcgccg agaagcaggg
    20041 catgggtggc ttcctgagca tcggcaagag ccgtgccaag gtattcatgg agaagaacac
    20101 tggcgtgacc tttgccgatg tcgctggcgt cgatgaagcc aaggcggagt tggttgagat
    20161 cgtcgatttc ctcaagaatc cgcaggagta tggacggctc ggggcgcgca ttcccaaggg
    20221 cgtgttgctg gtcggcccac cgggcaccgg caagacattg ctggccaagg ctgttgccgg
    20281 cgaggcggcg gtgcccttct tctccatctc cggatcagag tttgtcgaga tgttcgtcgg
    20341 cgtgggtgca gcgcgcgtgc gcgacctgtt cgagcaggcc cgcgggcagg cgccggccat
    20401 catcttcatc gacgagctcg atgcgctggg ccgcgcgcgc ggcgtcggcg gccccatcgg
    20461 cggccacgac gagcgcgagc agaccctcaa ccagctgctc acggagatgg acggcttcga
    20521 cagctcggtc gggctgatca tcctcgccgc caccaaccgc cccgaaatcc tcgaccaggc
    20581 gctgctgcgc gccggtcgct tcgaccgtca ggtgctggtg gaccggcccg acaagaaggg
    20641 acggctggac atcctgaaag tccacgtcaa gaaggtgacg ctggctcagg atatcgatct
    20701 cgaacaggtg gctgcgctga ccacgggctt ttcgggtgca gacctcgcga acctggtcaa
    20761 cgaggccgcg ctggccgcga cccggcgcaa agcgtccgcc gtggagttgc aggatttcac
    20821 cgccgccatc gagcgcatcg tggcgggcct ggagaagaag aaccgcgtgc tcaatcccaa
    20881 ggagcgggaa accgtggcct atcacgagat gggccatgcg ctggtggcgc tggcgctgcc
    20941 cggcaccgac cccgtgcaca agatctcgat catcccgcgc ggcatcggcg cgctgggcta
    21001 cacgctgcaa cgccccaccg aagaccgctt cctgatgacg cgcgccgatc tggaacacaa
    21061 gatcgccgtg ctgctgggcg gtcgtgcggc cgagaagctc gtgttcggcg agctgtccac
    21121 cggggctgcc gatgacctcg cacgggccac cgacattgcc cgcgacatga tcacccgctt
    21181 tggcatggac gaaggactgg gctacatcgc cttcgaggcg cagcggccac gctttctcga
    21241 tacaccggaa ctggcccacg gcggctgccg ggtggccgaa tcgacccagg cgcgcatcga
    21301 tcaggccatc cgcgacatcg tgatgggcgt gttcgagcgc gcctaccgga tcctcgacac
    21361 caaccgcgcg gtgctggagc gttgtgcgcg cgaactgctg gcgcgggaaa cgctcgacga
    21421 gaacgacatc cttcaattga cccaaggact tgttctagat agaaacgaaa agtagtaggc
    21481 gctattcaga gtgagccggc ggctctgttc ggtgcgtcat ccaattcgcc ggcattgttc
    21541 aaggagaacc gatatgtctg cattgactcc gtgggacccc ttccgggaac tggatgaatt
    21601 gcaaaaccgc ctggcgacga tgttgggacg gacaccccag cgacagggcg cccgtaccgg
    21661 caacgaagcc ctgaccacgg cggactgggc accaatggtg gacatcagcg aggatgagaa
    21721 cgcattcctc ctcaagctgg atctgccgga ggtccccaag gatgccgtgc gcgtcagcgc
    21781 ggaaaacggc gtgctcacca tcagcggcga gcgcaaactg gaaaaagagg agcagggcaa
    21841 gaaattccac cgcatcgaac gtgcgtatgg ccgctttgtg cgcagctttg tcttacctga
    21901 caacgttgat ccgaccaagg tgacggcttc gatgaaagac ggcgtgctgg aagtgcggct
    21961 tgtcaaggcc gagcaagcca agccgaagca gattgaaatt tcagtcaatt aacttcaatt
    22021 ggagtccaac atcatgaata tcaaacagcc ccaatacatc cgttccgcac ttgccctggc
    22081 ggtctgcatc ggactgagtg gaccggtttt ggcccaatcg gcggcaagtc caagcgctgc
    22141 agcgccctct gtcgcaccca aggcggcgca gccacaagta gacgacaagg ccgcccagga
    22201 agccgaaaaa aagcgttccg agctcactca ggacgccatc acggcgctca ccaagaccca
    22261 ggaggctttg accctccttg atgcaaataa gaccaaggag gcgctcgctg cgctggaact
    22321 ggccaccgga aagctggaac tggtattggc acgcgacgcc aaacttgctt tggcgccggt
    22381 cgatgtacgc gtcatcaccc atgatatcca cgccaacgtg gaatcggtaa agaaagcggt
    22441 caagttgtct cgggagttgt tgggtgatgg cgaggtgcaa aaggcccggc ccatcgttgc
    22501 caatctggcc agcgaaatcg tgatcgaaac cgacaacctg cccatggcaa cgtacccggc
    22561 agcgatcaag tcggctgcac ggctcgtcga cagcggcaag atcgacgaag ccaaagcaga
    22621 gctcgcccga gcactgaaca cgctggtggt cacccaagtt gtactccctc tgcccgtgct
    22681 acgggccgaa gccgcgatag caaaagctga aaagctggcc gagactgaca agcgcgatgc
    22741 caagcagaac gaggagctca gcaccttgct gtcgtccgtg cgcacggaga tcgagctggc
    22801 gcaaatcctg ggttatggca agaaggagga cttcaaaccc atcttcgatc aggtgaagtc
    22861 cattgagcaa aagtcggctg gtggcaaaag cggcaatgga tggttcgacg agttgaagac
    22921 gcgcatccaa aagctgtttt gaggtgatga aggggccgac tgctccatcg gtcagtctcg
    22981 cgatattcgc agtcggcccc aacagattcc cctattttca ctatctcata cgaggcatat
    23041 caccatgaat gagcaaacat cgaatcccaa cgcgatcaac gaaggcacga acgaacaggc
    23101 cgcggtaagc agccttcctg ccagaccaga gcccaagcct gaagtcgtca cggaggtaca
    23161 gcctgaggtt cagaaggaaa cggactcgca ggcggcagac aaacgtaaac aagtcctcga
    23221 tgaggccgtc tcggccttgg cgctgaccaa atcagcgctg gcggccctgg acgaaaaaga
    23281 atccgcgcga gcactggcaa cgctggccga agtgacggga aagctggagc tgatcgttgc
    23341 acgcgaaccc acgctcgccc tggccc
//
LOCUS       DAPHBJ010000059        23025 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.059, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000059 DAPHBJ010000000
VERSION     DAPHBJ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.059"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            56..1309
                     /locus_tag="RHZ76_002042"
     CDS             56..1309
                     /locus_tag="RHZ76_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2812684.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1706..2041
                     /locus_tag="RHZ76_002043"
     CDS             1706..2041
                     /locus_tag="RHZ76_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HDV2812685.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAKKHSHQQP
                     AKPAAQPAA"
     gene            2134..2217
                     /locus_tag="RHZ76_002044"
     CDS             2134..2217
                     /locus_tag="RHZ76_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812686.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            2317..3138
                     /locus_tag="RHZ76_002045"
     CDS             2317..3138
                     /locus_tag="RHZ76_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDV2812687.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLSLTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(3177..3506)
                     /gene="mdtI"
                     /locus_tag="RHZ76_002046"
     CDS             complement(3177..3506)
                     /gene="mdtI"
                     /locus_tag="RHZ76_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HDV2812688.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(3493..3858)
                     /gene="mdtJ"
                     /locus_tag="RHZ76_002047"
     CDS             complement(3493..3858)
                     /gene="mdtJ"
                     /locus_tag="RHZ76_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HDV2812689.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            4270..5304
                     /gene="tqsA"
                     /locus_tag="RHZ76_002048"
     CDS             4270..5304
                     /gene="tqsA"
                     /locus_tag="RHZ76_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HDV2812690.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(5329..6717)
                     /gene="pntB"
                     /locus_tag="RHZ76_002049"
     CDS             complement(5329..6717)
                     /gene="pntB"
                     /locus_tag="RHZ76_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HDV2812691.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(6728..8260)
                     /gene="pntA"
                     /locus_tag="RHZ76_002050"
     CDS             complement(6728..8260)
                     /gene="pntA"
                     /locus_tag="RHZ76_002050"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HDV2812692.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCTCSPWRKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            8784..9728
                     /locus_tag="RHZ76_002051"
     CDS             8784..9728
                     /locus_tag="RHZ76_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV2812693.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            9914..11296
                     /locus_tag="RHZ76_002052"
     CDS             9914..11296
                     /locus_tag="RHZ76_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV2812694.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            11333..12055
                     /gene="folM"
                     /locus_tag="RHZ76_002053"
     CDS             11333..12055
                     /gene="folM"
                     /locus_tag="RHZ76_002053"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HDV2812695.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(12052..12387)
                     /locus_tag="RHZ76_002054"
     CDS             complement(12052..12387)
                     /locus_tag="RHZ76_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HDV2812696.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            12516..13235
                     /gene="rstA"
                     /locus_tag="RHZ76_002055"
     CDS             12516..13235
                     /gene="rstA"
                     /locus_tag="RHZ76_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HDV2812697.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            13239..14540
                     /gene="rstB"
                     /locus_tag="RHZ76_002056"
     CDS             13239..14540
                     /gene="rstB"
                     /locus_tag="RHZ76_002056"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HDV2812698.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNKATLIVEDDGSGIAPENREHIFEPFVRLDPSRGRSTGGCGLGLA
                     IVHSIALAMGGTVNCGTSELGGARFSFSWPLWHNIPQFTSA"
     gene            14616..15545
                     /gene="tus"
                     /locus_tag="RHZ76_002057"
     CDS             14616..15545
                     /gene="tus"
                     /locus_tag="RHZ76_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HDV2812699.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGK
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(15542..16945)
                     /gene="fumC"
                     /locus_tag="RHZ76_002058"
     CDS             complement(15542..16945)
                     /gene="fumC"
                     /locus_tag="RHZ76_002058"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDV2812700.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEYNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(17088..18734)
                     /gene="fumB"
                     /locus_tag="RHZ76_002059"
     CDS             complement(17088..18734)
                     /gene="fumB"
                     /locus_tag="RHZ76_002059"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDV2812701.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            18933..20108
                     /gene="manA"
                     /locus_tag="RHZ76_002060"
     CDS             18933..20108
                     /gene="manA"
                     /locus_tag="RHZ76_002060"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HDV2812702.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            20209..21717
                     /locus_tag="RHZ76_002061"
     CDS             20209..21717
                     /locus_tag="RHZ76_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDV2812703.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLATQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(21762..23009)
                     /gene="uidC"
                     /locus_tag="RHZ76_002062"
     CDS             complement(21762..23009)
                     /gene="uidC"
                     /locus_tag="RHZ76_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HDV2812704.1"
                     /translation="MAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRADKPSAG
                     AGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGDKSFGFA
                     LGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALGYWALTP
                     NIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDTSRYSLG
                     ASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETALVSGQL
                     TWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVNYRLTPQ
                     FTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKGREKRDG
                     STLGDRLNYWDVKMSIQYDFMLK"
BASE COUNT         5690 a         5942 c         5552 g         5841 t
ORIGIN      
        1 ttttcctacc ctatgtataa gcctgatcta caggcatatt tagcaaggat ttcaagtgag
       61 ccgtactaca actgttgatg gcgctccggc aagcgacact gacaagcaaa gcatttctca
      121 gccaaatcaa tttattaaac gcggtacgcc gcaatttatg cgcgtcaccc tggcgctgtt
      181 ctctgccgga ctggcaacat ttgcacttct ctattgtgtg cagcctatcc ttccggtgct
      241 ttcgcaggag tttggcttaa cccccgcgaa cagtagtatt tcactgtcca tttccacggc
      301 gatgttggct attggtttgc tgtttactgg cccgctatcc gatgccattg gtcgcaaacc
      361 agtgatggtc acggcgctac tgttggcctc catttgtacg ttactttcga caatgatgac
      421 cagctggcac ggcattttga ttatgcgcgc cttgattggg ctttcgttaa gtggcgtggc
      481 agctgttggc atgacttatc ttagcgagga aatccatccc agtttcgtgg ccttttcaat
      541 ggggttgtat atcagcggca actcaattgg cggcatgagc ggacgcttaa ttagcggtgt
      601 cttcacggac tttttcaact ggcgaattgc tctggcggca atcggttgtt tcgcgctggc
      661 ctcggcgttg atgttctgga aaatcctccc tgaatcacgc cattttcgcc cgacttcgct
      721 gcgccctaag acgttgttta tcaactttcg tctgcactgg cgtgaccggg gattaccgtt
      781 attgttcgca gaaggctttt tgctgatggg gtcgttcgtc acgctgttta attacatcgg
      841 ctatcggttg atgctctccc cctggcatgt cagtcaggcc gtggttggct tattatcgct
      901 ggcttatttg accggtacat ggagctcacc caaagccgga accatgacca cccgctatgg
      961 gcgtggtcca gtgatgttgt tttcgacggg ggttatgctg tttggtttac tgatgacctt
     1021 attcagctcg ctgtggctga tctttgccgg aatgttactc ttctcagcag gattcttcgc
     1081 agcccactca gtagccagca gctggatcgg cccccgcgca aaacgcgcta aaggccaggc
     1141 ctcctcgctg tatctgttca gttactatct ggggtcgagt attgccggga cgctgggtgg
     1201 tgttttctgg cataactatg gctggaacgg cgtcggcgca tttattgctc tgatgctggt
     1261 cattgctctg ctggtcggga cgcgtttgca tcgtcgtctg cacgcctgaa aaataagtcc
     1321 ggactgcggt aaatacccgt ccggacttat tgccagctca aaccaacgtt aatagccatc
     1381 ctaaaataga cgaagcgcca gccaattccc gcagcgcatc tagcgtcata aggattataa
     1441 gtacccaaat aaacggattc attttgctgt gtgtcattta ttactgatgc gcagttattc
     1501 taccgctttg taagtagtaa aatagttaac ccgatcaaga ctactattat tggtagctaa
     1561 attttcctta agtcacaata cgttattatc aatgctgtaa tttattcagc gtttgtacat
     1621 atcgttacac gctgaaacca accactcacg gaagtctgcc attcccaggg atatagttat
     1681 ttcaacggcc ccgcagtggg gttaaatgaa aaaacaaatt gagggtatga caatgaaaaa
     1741 agtattagct ctggttgttg ccgctgctat gggtctgtct tctgccgcct ttgctgcaga
     1801 gactgcgacc acacctgctc cgactgcgac gaccaccaaa gcagcgccgg cgaaaactac
     1861 acatcataaa aaacagcata aagcagcacc tgcccagaaa gcgcaggcgg ctaaaaagca
     1921 tcataaaaat acgaaagctg aacagaaagc ccctgaacaa aaagcgcagg cagcgaagaa
     1981 acacgccaag aaacacagcc atcagcaacc ggcaaaacct gctgcacaac ccgcagcgta
     2041 agttttcaac agtaatgctg gcgcgccccc tcgcgcctga aaattacggt gctaagcggg
     2101 taacgtttag caccgccttt agcggagggt agtatggtgg gccgttatcg ctttgagttc
     2161 attcttatca ttcttatttt atgcgcactg attgccgccc gtttttatct ttcctgactg
     2221 tagtcatctg attttactcc cactttcatc ccgtcccgtc tatagtattt atgagggttt
     2281 gcttttaata atcataatta cccaccagag tgtgatatgc gtacaaccat tgctgtagtg
     2341 ttgggtgcaa ttagtttgac gtctgctttt gtgtttgcag ataaaccaga cgttgccaaa
     2401 tcggcaaacg atgaggtcag caccctgttt tttggtcatg atgatcgtgt gccagtgaat
     2461 gacacgaccc aatcaccgtg ggatgcggtt gggcaactgg aaacggccag cggcaattta
     2521 tgtacggcga cgctgattgc acccaatctg tcattaacgg caggacactg tttattgaca
     2581 cctccaaagg gtaaagccga taaagcagtg gcgctgcgtt ttgtgtcaaa taaaggtctt
     2641 tggcgctatg agatccacga catagaaggc cgcgttgatc cgacactggg aaagcggtta
     2701 aaagcagatg gggatggttg gattgtacct cccgcagccg cgccgtggga cttcggattg
     2761 attgtgctac gtaatccccc ttctggcatt acgccgttgc cgttatttga gggagataaa
     2821 gccgcgctta ccgccgcatt aaaagcggca ggtcgtaaag tgactcaggc aggctaccct
     2881 gaagatcatc tcgatacgtt gtacagtcat caaaactgtg aagtgactgg ctgggcgcaa
     2941 acgtcggtga tgtcacatca gtgcgatacc ttgccgggtg acagcggttc gcctctgatg
     3001 ttgcataccg atgacggctg gcaattaatt ggggtgcaaa gttcggctcc tgccgcgaaa
     3061 gatcgctggc gcgccgataa ccgggccatt tctgttaccg gttttcgcga caagctggat
     3121 caactgtcgc aaaaataatg ttcaaacgct gcccgacagc gcgggcagcg tcttcatcag
     3181 gcaagtttca ccatgatcat tccagccaac agcaagacca ggccaatcca gcctttacga
     3241 tttaaccgtt gaccaaacaa gatccaacct gcggctaacg tggcggcaat accaaacccg
     3301 ccccacaatg cataagcgac agacaagtcg atccctttaa cggcttgaga aagcgcacta
     3361 aaggcagcca gcaccgccgc cagggagagc aagccaaata ttttgcgacg aaagccgtca
     3421 gaaaatttca aaaagacgtt agcaacgatt tccagcacga ttgccaatgc cagccaggcg
     3481 gcgtgaaccc attcaaactg cgccatggtt cacctccagt tcaggtttac gcgctttacg
     3541 ggtacctgat tttatcaaca caatcccggc gaccagggtg gttaacccgg caattttcat
     3601 cagcgataaa ctttcgtcga ataacaaaac gctaaacaag gtaataaata aaataccgat
     3661 accttcccac agcgcataag ctacgcctaa ggcgattttt ttaacggcga aagagagaaa
     3721 tatatacgac agagaaatca tcaccagcat taaaataaag ccgccatttc cctcactgac
     3781 gctcgcccat ttcattgaca gcgtaccggt aatttctgta gcaatagcca gacctaataa
     3841 aatccaataa atatacattg tccttctcct gcaagagaat tattttaatt ttcgcttaat
     3901 tcagcgaaac caaagtaaag tggtcaagcc ccgcgcatag cgcaagcttt ggcagaagaa
     3961 aggactaaag cgcgttgcgc cagtgctgct cacctacgag caagatagtg gatgaggtac
     4021 gaagatgggt aaatgtagaa aataacgtcc tgaacaaatt gtccataata ttacaattat
     4081 ccgcagtgtt gcttctcgtc atcgcggatg ataattgtcc tcggtagttg aacacgcctg
     4141 atttgtatca tagcttaaga attaactcaa aatattttca cttctttacc tgagcggttt
     4201 gattttcgtt atgatgacgg agcgaaaaag acattattat tagcaaagga agaaaaaacg
     4261 gggacaagca tggcaaagcc gatcatcacg ctcaatggcc taaaaatcgt cattatgttg
     4321 ggaatgctgg tcattattct ctgcggtatc cgttttgccg ccgagatcat cgtgccgttt
     4381 attctcgcat tatttattgc tgttattctt aacccgctgg tgcaacacat ggtccgctgg
     4441 cgtgtgccgc gtgtactggc ggtgtcgatt ttgatgacca tcatcgtgat ggcgatggtg
     4501 ttgctattag cttatctggg ttccgcgctc aacgagttga cgcggacgtt accgcaatat
     4561 cgcaactcta ttatgacgcc gctgcaagcg cttgaaccgt tgttgcaacg cgtagggatt
     4621 gacgtctcag ttgaccagct ggcgcattat attgatccga acgcggcgat gacgttgctc
     4681 accaacttat tgacgcagtt atctaatgcc atgtcatcaa tatttttatt gctgctgacg
     4741 gtgctgttta tgctgctcga agtgccacaa ttgcccggaa aatttcagca aatgatggcg
     4801 cgtccggttg aagggatggc ggcgattcaa cgtgcgattg acagtgtttc tcattatctg
     4861 gtgctgaaaa cagccatcag catcatcacc ggcctggtcg cctgggcgat gctcgccgca
     4921 ctcgatgttc gcttcgcttt tgtctgggga ttgctggcct ttgcgcttaa ttacatcccg
     4981 aatattggtt cagtcctcgc ggcaatcccc cctatcgctc aggtactggt gtttaatggc
     5041 ttctacgaag cgttgctggt gctggcggga tatctgctga ttaatctggt cttcggcaat
     5101 attctggagc cgcgtatcat ggggcgtggg ctggggcttt ccacattggt ggtatttttg
     5161 tcgttgattt tttggggatg gttgttagga ccggtgggta tgctgctttc cgtgccgttg
     5221 acaattattg tcaaaattgc gcttgaacaa acagcgggag gtcaaagcat cgccgttctg
     5281 ttaagcgatc tcaataaaga gtgacggcct cagcagaggc cgtcagggtt acagagcttt
     5341 caggattgca tccacgctgg ctttggcgtc accaaacagc atgtgggtgt tttccttgaa
     5401 gaacagcggg ttttgcacac cagcatagcc agtgttcatc gaacgtttaa agacaatcac
     5461 gttctgcgct ttccacactt ccagcacagg cataccagca atcggactct tcggatcatc
     5521 ctgcgccgcc gggttaaccg tatcgttagc accaatcacc agtacggtat cggtatcagc
     5581 aaagtcatca ttgatctcgt ccatttccag cacgatgtca tacggtactt ttgcttcagc
     5641 cagcaatacg ttcatatgtc caggcaaacg ccccgcgacc gggtggatac cgaaacgcac
     5701 attaatacca cgagcgcgca atttctcagt aatttcagcg acaggatatt gcgcctgcgc
     5761 gactgccatg ccgtaccccg gagtaatgat cactgaatgg gagtttttca gcagttccgc
     5821 tgtctcttct gcggtgattt cgcggtgctc acccacttcc tgatcatcgc cagtagaaga
     5881 gccgtcggtg ccgaaaccac ccgcaataac gctgataaag gaacggttca tcgccttaca
     5941 cataatgtaa gaaaggatag cccccgaaga accgaccagc gcaccggtca caatcagcag
     6001 gtcgttgctg agcataaagc ccgcagccgc agccgcccag ccggagtacg agttcagcat
     6061 cgacaccacc actggcatat ctgcaccacc gatggaggcg actaaatgcc agccgaatac
     6121 cagcgcaatt gcggtcatta tcagcaatgc cagcacttgc aggccgacgc tgtccgtgcg
     6181 aacaaataca atcagcagca ggaaggaaac gaccagagcc gccaggttca ttttgtgacg
     6241 gtttggcagc atcaatggtt tagacgaaat cttgccacac agtttgccga acgccaccac
     6301 cgaacccgtg aacgttaccg ccccgatgaa gataccgagg aacacttccg tcaggtgaat
     6361 attgaccaga atcggtgcca ttcccgcgtc atgatgcaga tagctgttaa agccaaccag
     6421 cactgccgcc agacccacga agctatgcag gatcgccacc agttctggca tttcggtcat
     6481 ttcaactttc ttcgccagac ggataccaat tgccccacca atgaccatcg ccagcaagat
     6541 ccagccaaca ttacccgtat ccggtccaaa aatggttgcg attaacgcaa tcgccatccc
     6601 ggcgataccg aagttgttac cctggcgaga cgtttcatgt ttcgaaagac cggccagact
     6661 gaagataaac aggatcgcgg caacaatgta tgcagctgta actaatcctc cagacatatg
     6721 ttacccctta atttttgcgg aacattttca gcatgcgctg agtcacggtg aagccaccga
     6781 aaatattaat gctggctata agcaccgcga taaaactaag gaagctaacc cagccgccct
     6841 ggccaatctg caacagtgct ccgacaacaa taatccctga aatcgcgttg gtgaccgaca
     6901 tcaacggtgt atgcagcgcg tgcgatacat tccacaccac gtaataaccg acaacgcagg
     6961 ccagcgcgaa aacggtgaag tgcccaagga attctttcgg cgcaacgctt gccatccagc
     7021 caaaaagaat gattgccagc gccatcaacg cgtatttacg ccacggtgag caggtacatt
     7081 tttcctcagt tttcacttcc ggtgccgctt tttgtgccgc ctgcggctga gctgatacct
     7141 gaatcggcgg tgccggccag gtaatttcgc ccgcacggat cacggtcacg ccgcgaatca
     7201 ccacatcatc aaaatcaaca gtgatattgc cgtctttctc tttgcacaac agtttcagca
     7261 gattaacgag gtttgtgccg taaagctgtg aggattgcgt cggcagacgg cccggaagat
     7321 cggtataacc aatcactttg acaccatttt ccgtagtgaa gatttcaccc ggcacggtgt
     7381 attcacagtt gccgccgttt tgggctgcca ggtcgacaat cacactgccc gccttcatgg
     7441 agtcaaccat ttcacgggta attagcttcg gcgctggttt gcctggaata agcgcggtgg
     7501 tgacaatgat atcgacctct tttgcctggg cggcaaagag ttccatttcc gctttgatga
     7561 acgcgtccga catcactttg gcatagccat cgccgctgcc agcttcctct ttaaaatcca
     7621 gctcgaggaa ttccgcgccc atactttgaa cttgttcttt cacttccggg cgggtgtcga
     7681 atgcacgcac aatcgcgccg agactgtttg ctgcgccaat ggcggccaga cctgcaacac
     7741 ccgcaccaat caccatcact tttgccggtg gcactttccc ggccgcagta atttgcccgg
     7801 taaagaagcg cccaaattca tgtgccgctt caacaatggc gcgataaccg gcgatgttcg
     7861 ccatcgagct tagtgcgtcc agcgattgtg cgcgtgagat acgcggcaca gagtccatcg
     7921 ccatcacggt cacgttacgt tccgcaagtt tttgcattaa ttccggattc tgcgcaggcc
     7981 agataaaact caccagcgtt gtcccaggat tcagtaacgc aatttcatca tctaacggcg
     8041 cattgacctt cagaatgatc tctgactgcc agacgctatt cccttctaca atttcagcgc
     8101 ccgcttgcac aaacgcttta tcgtcaaaac ttgccagttg acccgcgccg ctctctaccg
     8161 cgacggtaaa acccagtttc agcagctgtt ccactgtttt tggcgttgct gcaacacggg
     8221 tttcattggt taaccgttct cttggtatgc caattcgcat gatattccct tccatcggtt
     8281 ttattgatga tggtttgcct gtgtcaggag ccacacaagc tgctcatgta cgagctaaat
     8341 gttactccgt taaaataaat tagtaacaaa cgccctataa cgtactgaaa attatgcctg
     8401 tgatctagcg ccaaaaatca gtatttcggc gtgaactcgc aaaatattaa cgattcagcc
     8461 gtgatagtgg gataaacacc ttagaacgcc ggataaagac tgataattgt cttcgacggt
     8521 cgggtaaaac gagacatcgc cccggcacga atcactactt aacattaaat taacttatac
     8581 aattcagttg cttcagtagt aatgatgctg atacggctgt tttttaagca tagacggtca
     8641 tttgagcagg attaaaattg gcttaaggaa tgtgatatga aaaatgacgc agacagttac
     8701 accgtttaaa tgcaataatc agccacgttt ctcgttaata acaataccag tacctggttt
     8761 gcgcaaggcg aaggattatt tttatgaagc ttaagaacac cctcctggcg tcggcactgc
     8821 tttctgctac ggcattctcc gttaacgcag caacagaact gacaccggag caagcggcag
     8881 cggttaaacc ttttgaccgt gtagtggtta ccggtcgttt taatgctatt ggcgaagcgg
     8941 tgaaagccgt ttctcgtcgc gcagataaag aaggtgccgc ctctttttat gttgtcgaca
     9001 cttctgattt tggtaacagc ggtaactggc gtgtggtcgc tgacctctat aaagccgatg
     9061 ctgaaaaagc agaagaaaca agtaatcgcg taattaacgg tgttgtcgaa ctgccgaaag
     9121 atcaggctgt tctgattgaa ccgtttgaca cggtcaccgt ccagggcttc tatcgtagcc
     9181 agccagaagt caatgatgcc atcaccaaag cggcaaaagc gaaaggtgcc tactctttct
     9241 acatcgttcg tcaaatcgat gccaaccagg gcggcaacca gcgtattact gcattcatct
     9301 ataaaaaaga tgctaagaaa cgtatcgtcc agagcccgga tgtgatcccg gcagattccg
     9361 aagcaggacg tgcagctctg gctgccggtg gcgaagccgc gaagaaagtt gagatcccgg
     9421 gtgttgcgac taccgcatca ccaagttctg aagtcggtcg cttctttgaa acccagtcat
     9481 caaaaggcgg gcgttacacc gtcacgctcc cggatggcac taaagtcgaa gaactgaaca
     9541 aagcgacagc agcgatgatg gtcccgttcg acagcatcaa attctctggc aactacggca
     9601 acatgaccga agtctcctat caggttgcga aacgtgccgc gaagaaaggt gctaagtatt
     9661 accacatcac ccgccagtgg caggaacgtg gtaataacct gaccgtcagc gcagatctgt
     9721 ataaataact attcagttac tcctataagg cggcttgatg ccgcctttcg catttttagt
     9781 cacaaatttc accgcgacca ttgcatgcag acacatctct tcgtaaaatc ccgcgcttca
     9841 gtggcaaact ccatttttat gcgttttgcc ctaatattta ttcttcttat cacgttttaa
     9901 tcactggata tcgatggaaa agaaactggg actgagcgca ctcaccgcgc tggtattaag
     9961 ctcaatgctg ggcgcgggtg ttttcagtct gccgcaaaat atggcggcag ttgccagccc
    10021 ggcagcactg ctcatcggct ggggtattac tggcgctggc attttattgc tggcctttgc
    10081 catgctgatc ctcacgcgca ttcgtcccga acttgacggc ggtatcttta cctatgcccg
    10141 cgaagggttt ggcgagctaa tcggcttttg ttccgcatgg ggatactggc tgtgcgcagt
    10201 catcgccaac gtctcctatc tggtgatcgt tttttccgcg ttaagctttt ttacggacac
    10261 gccggaattg cgcctgttcg gcgatggtaa tacctggcaa tcgatcgtcg gtgcatcggc
    10321 tttattgtgg attgttcact ttttgatcct gcgcggggtg caaaccgctg ccagcattaa
    10381 cctggtggcg acactggcaa aattgttgcc gctgggtctg tttgttgtgc tggcgatgat
    10441 gatgttcaaa ctggatacct tcaagctcga cttcaccgga cttgcacttg gcgtacccgt
    10501 gtgggaacag gtgaaaaaca ccatgctgat caccctgtgg gttttcattg gtgtggaagg
    10561 tgccgtggtt gtttctgcgc gtgcgcgtaa taaacgtgat gttggcaaag cgacactgct
    10621 ggcggttctc tccgctctgg gcgtttactt gctggtaacg ctgctttcgc tgggcgtggt
    10681 ggcacgtcct gaactggctg aaattcgtaa cccgtcaatg gccggtctga tggtggaaat
    10741 gatgggacca tggggcgaaa tcatcatcgc tgccggtttg atcgtttccg tttgcggcgc
    10801 gtacttaagc tggaccatca tggcggcaga agttccgttc ctggctgcca ctcataaagc
    10861 attcccacgc atttttgcgc gccagaacgc acaagctgcg ccatccgcct cgctgtggtt
    10921 gaccaacatc tgcgtgcaaa tttgtctggt actcatctgg ctgaccggtt ccgattacaa
    10981 cacgttgctg accatcgcct cagaaatgat tctggtgccc tatttcctgg tcggcgcgtt
    11041 cttgctgaaa atcgcaaccc gtccactgca taaagcggta ggtgtcggtg cctgcattta
    11101 tggcttatgg ttattgtatg cctctggtcc aatgcacttg ctgttatccg ttgttctgta
    11161 tgcgccgggg ctgctggttt tcctgtatgc gcgtaaaacg catacacatg ataacgtact
    11221 gaaccgccag gaaatggtgc taattggcat gctgttaatt gcctcagttc cggcgacctg
    11281 gatgctggtg ggataacgtt catcccatcg ttggttagca aaggagataa cgatgggtaa
    11341 agcccagccc ttgccaatat taattactgg cggtggtcgt cgcatcggcc tcgccctcgc
    11401 atggcatttc attaatcaaa agcaaccggt gattgtcagc tatcggacac actatccagc
    11461 cattgatgga ctgattaatg caggtgcgca gtgtattcag gctgattttt cgaccaacga
    11521 cggtgtgatg gcgtttgccg atgaagtact aaaaagcacc catggtctgc gtgctatttt
    11581 gcataacgcc agtgcgtgga tggcggaaaa accgggtgcg ccactggccg acgtactggc
    11641 ttgcatgatg cagatccacg ttaatacccc atacctgctc aaccatgcgc tggaaagatt
    11701 actgcgtggg cacggacacg ccgccagcga tatcattcac tttaccgatt atgtggtgga
    11761 gcgcggtagc gacaaacata ttgcgtatgc tgcaagcaaa gcggcactgg ataatatgac
    11821 ccgctcgttt gcccgcaagc tggcaccgga agtgaaagtg aattctattg cgccatcgct
    11881 gatcctgttt aatgaacatg atgatgccga atatcgacaa caggcgctga ataaatcact
    11941 gatgaaaacc gcgcctggcg agaaagaagt gatcgacctg gtcgattact tacttaccag
    12001 ttgctttgtc accggacgca gtttcccact tgatggcggt cgtcatctgc gttaatgcag
    12061 ctttatccag catataatca acacccatgc gcttatcccc caacacgcaa cagaaccaac
    12121 aaaagcggcg ggcaagcgca tcattccggt gaaataccac agcgacacca ggtaaacaaa
    12181 atagggaata atcgaccaca tactaaaaat gatggttgcg cgtaaggctt caatgccgcg
    12241 ttcgctggca acaatataat gcgcgataag cgcaaaggtc gggaaaagtg gaatcagccc
    12301 ggcgatataa taatttttcg tttttgctaa aacaccaatc aacagcacta ccagcgcacc
    12361 gagcgcggct ttgattacca gccccatctt tttaccttaa cacttccata acaagtcatc
    12421 agtagaatac ctgatgaaaa cttgtttaga aacgattgat agtaagtaaa aacagcgcgg
    12481 tgtattgtga cgtttttata tctaccgtga atgttatgaa cactatcgta tttgtggaag
    12541 atgatgcgga agtcggttca ctgattgccg cgtacctggc aaaacatgat atgcaggtta
    12601 ccgtagagcc gcgcggcgac caggccgaag aaaccatttt gcgagaaaat ccggatttgg
    12661 tgttactcga catcatgcta ccaggcaagg acggcatgac catttgtcgt gatttacgcg
    12721 caaagtggtc tggaccgatt gttcttctaa cctctctcga tagcgatatg aaccacatcc
    12781 tggcactgga aatgggtgcc tgcgactata ttctcaaaac gacgccccct gctgttttgc
    12841 tagcgcgttt acgtttgcat ttgcgtcaga atgagcaagc cacactgacc aaaggtcttc
    12901 aggaaacgtc tctgactccc tacaaagccc tgcatttcgg cacgttgacc atcgatccca
    12961 tcaaccgcgt agtcaccctg gctaacactg aaatctcgct ctcgacagct gatttcgaat
    13021 tattgtggga attagctacc catgccgggc aaatcatgga ccgcgatgca ttgctgaaaa
    13081 atttacgcgg cgtcagttat gacggactgg atcgtagcgt ggacgtggct atttcgcggt
    13141 taagaaaaaa actactcgat aacgccgcag aaccttatcg cattaaaacc gtgcgtaaca
    13201 aaggctatct ttttgcgcct catgcatggg aataagcgat gaaaaaattg tttatccagt
    13261 tttacctgtt attgtttgtc tgcttccttg tgatgtctct gctggttggg ctggtgtaca
    13321 aatttaccgc cgaacgcgcg ggcaaacagt cgctggatga tttgatgaac agttcgctgt
    13381 atctgatgcg cagcgaattg cgtgagatcc ccccacacga ctggggtaag acgctgaaag
    13441 agatggattt aaatctctct ttcgatctgc gtgtcgagcc actgagtaaa taccatcttg
    13501 atgatatttc catgcaccga ctgcgtggcg gcgaaattgt cgccctggac gatcagtaca
    13561 cgtttttgca gcgtatcccg cgcagccact acgtgctggc agttggtcct gttccttatc
    13621 tttattacct ccatcaaatg cgattgctgg atatcgccct gatcgctttt attgctattt
    13681 ccctcgcctt tccggtgttt atctggatgc gtccgcactg gcaggatatg ttaaaactgg
    13741 aagcagcggc gcaacgattt ggcgatgggc atctcaatga acgtatccac tttgatgagg
    13801 gttcgagctt tgaacgactt ggcgtcgcat ttaaccagat ggcggacaat atcaacgcct
    13861 taattgccag caaaaaacag cttattgacg gtatcgctca cgaactgcga acaccgttag
    13921 tgcgcctgcg ttatcgactg gagatgagcg ataacctgag cgccgccgaa tcccaggcgt
    13981 tgaatcgtga tatcagtcaa cttgaagctt taattgaaga gctgctgact tatgcccgac
    14041 tcgatcgccc acaaaacgag cttcatctta gcgaaccaga cctgccgttg tggctgtcaa
    14101 cgcatctggc agatattcag gcagtaacgc ccgataaaac ggtacggata aaaacgctca
    14161 tgcaaggcca ttatgcggcg ttggatatgc gcttaatgga gcgcgtgctg gataatttgc
    14221 tcaataacgc cctgcgctac tgccattcaa cggttgaaac cagcctgcta ctgtcgggga
    14281 ataaagcgac attaattgtt gaggatgatg gctcagggat tgccccagaa aaccgcgaac
    14341 atatctttga accttttgtt cgcctcgatc ccagccgggg tcgctcaacc ggcggctgcg
    14401 ggctgggact ggcaattgtc cactctatag cactggcaat gggcggtacg gttaattgtg
    14461 gcaccagcga actgggtggt gcccgcttct cgtttagctg gccgttatgg cataacatcc
    14521 cgcaatttac ctctgcttga cactacgcgc acggtggtca agtcaccacc actgtgctat
    14581 aaattaagga tgttgtaact aaagtggtta atattatggc gcgttacgat ctcgtagacc
    14641 gactcaacac tacttttcgc cagatggaac aagagctggc tgcatttgcc gctcatcttg
    14701 agcaacacaa gctattggtt gcccgcgtgt tctctttgcc ggaggtaaaa aaagaggatg
    14761 agcataatcc gcttaatcgt attgaggtaa aacaacatct cggcaacgac gcgcagtcgc
    14821 tggcgttgcg tcatttccgc catttattta ttcaacaaca gtccgaaaat cgcagcagca
    14881 aggccgctgt ccgtctgcct ggcgtgttgt gttaccaggt cgataacctt tcgcaagcag
    14941 cgttggtcag tcatattcag cacatcaata aactcaagac cacgttcgag catatcgtca
    15001 cggttgaatc agaactcccc accgcggcac gttttgaatg ggtgcatcgt catttgccgg
    15061 ggctgatcac ccttaatgct taccgcacgc tcaccgttct gcacgacccc gccactttac
    15121 gttttggctg ggctaataaa catatcatta aaaatttgca tcgtgatgaa gtcctggcac
    15181 agctggaaaa aagcctgaaa tcaccacgca gtgtcgcacc gtggacgcgc gaggagtggc
    15241 aaagaaaact ggagcgagag tatcaggata tcgctgccct gccacagaac gcgaagttaa
    15301 aaatcaaacg tccggtgaag gtgcagccga ttgcccgcgt ctggtacaaa ggagatcaaa
    15361 aacaagtaca acacgcttgc cctacaccgc ttattgcgct gattaatcgg gataatggcg
    15421 cgggcgtgcc ggatgttggg aagttgttaa attacgatgc cgacaatgtg caacaccgtt
    15481 ataaaccaca ggcgcagccg cttcgtttga tcattccccg gctgcacctg tatgttgcgg
    15541 attaacgccc ggctttcata ctgccgacca tctgttctgg ccgtacccag ctgtcaaact
    15601 cggcttcgct aagatacccc agcgcaaggg ccgcagcttt taaggtcagc ccttctttat
    15661 gcgctttttt ggcgatctcc gcggctttat cataaccaat gtgggtgtta agcgcagtca
    15721 ccagcatcag cgattcattg agtaattgat tgattcgctc acgattcggt tcaataccca
    15781 ctgcgcagtg tttgttaaaa ctttccatgc catctgccag caagcgcacc gattgcagga
    15841 aattgtggat caccattgga cggaagacgt tcagttcaaa gttaccggaa gcgcccccca
    15901 tgttgatcgc cacgtcgttc cccatcacct gacagcagag catggttaat gcctcacact
    15961 gtgttggatt cactttcccc ggcatgattg agctgcccgg ctcattttcc gggattgaga
    16021 tttcaccaat tccgcagcgc gggccagagg ccagccagcg gacatcattg gcgattttca
    16081 tcagtgacgc agccaaccct ttcaacgcgc cgtgcgcctg aaccagagca tcacaggtcg
    16141 ccagcgcttc aaatttgttc ggcgcggtaa caaacggtgc acaggtaatg actgccagtt
    16201 catctgctac gcgacgcgca tactccggat gggtatttag tccagtaccc accgctgtac
    16261 cgccaagagc cagttccgct acgtgaggca ggctgtattc gatatgtttg agattatact
    16321 cgagcatcgc tacccagccg gaaatctcct gccccagtgt tagcggcgtg gcgtcctgca
    16381 agtgggttcg accgattttg acgatatcgg caaatgcacg cgatttttca ctcagcgtct
    16441 gtgtcagggt tttaagctgc ggaatgagtt gcttgcgcag cgccagcagc gccgcaacgt
    16501 gcatcgccgt cggaaagaca tcgttggaac tttggctttt gttcacgtcg tcgttagggt
    16561 gaactttacg ttccatcccg cgcacgccgc cgagtaattc actggcccgg ttagccagca
    16621 cttcgttcat gttcatgtta ctttgcgtgc cggagccggt ctgccagata gccagcggga
    16681 attcgtcgtc atgctgtcct gccagtactt catccgccgc ctgccgaatg gcgctcgctt
    16741 tctcttcaga caacaagcct aaatcttcat taacttttgc cgctgcgcgc ttggttagcg
    16801 ccagcgcatg aatcagtgag gtgggcattt tctccgtcga aatgcggaaa tgctccagcg
    16861 agcgttgagt ttgtgcgccc cacagcttat ctgccgggac atcaatcgcc cccatcgaat
    16921 ctttttcgct gcgtactgta ttcatggcct gctcctcacc tgtttaattt tttctttctg
    16981 ttttgctttc gttaagcaac ttttagctca cttattattt actatttgat aacaaatgtt
    17041 tggtctttcg tgccatgtaa aaaaaccgcc ccgaagggcg gctctgttta tttcacacag
    17101 cgagtgcact gtgtgagttg tatctgctgg aagaagtcat ttcctttatc atccacaagg
    17161 ataaacgccg ggaaatcttc cacttcaatt ttccagatgg cttccattcc cagttccgga
    17221 tattcaacac attccaggct cttaatactt ccctgcgcca atacagcggc cggaccaccg
    17281 atactgccaa ggtagaagcc gccgtgtttt ttacaggcat ccgtcacctg ctggctgcgg
    17341 ttgcctttcg ccagcatgat catacttccg ccctgcgctt gcagttgatc gacataagaa
    17401 tccatccgtc cggcggtcgt tgggccaaga gaaccggagg cataaccttc cggcgttttg
    17461 gccggacccg cgtagtaaat cggatgatct ttaatgtact gcggcagccc ttcaccgtta
    17521 tccatccgct ctttcagttt ggcgtgagca atatcacgac cgacgataat cgtgccgtta
    17581 agcgataagc gtgtagaaac gggatactgc gacaactgtg cgaggatctc tttcatcgga
    17641 cggttaaggt caacgcgcac cgcttcgcct tctcccgctt tacgcagctc ttcagggata
    17701 tatttacctg gattatgttc cagtttttcg atccagatcc cctgacggtt gatcttcgct
    17761 ttgatattac ggtcagcaga gcaggaaaca cccataccaa ccggacagga tgcgccgtga
    17821 cgtggcaggc gaatcacgcg tatgtcgtga gcgaagtatt taccaccaaa ctgcgcaccc
    17881 agaccgagat tttgcgcttc gatcagcaat tctttttcca gttccacatc gcggaatgcc
    17941 tgaccgtgct cattcccttc cgttggcagt tcgtcatagt atttagcgga agccagtttc
    18001 actgttttaa ggttagtttc tgcagaagtt ccaccaatta cgaacgcaat atgatacgga
    18061 ggacaggccg ccgtacccag cgtgcgcatc ttctcaacca ggtaattttt cagtttcccc
    18121 ggcgttagta acgctttggt ttcctgatag agatacgtct tgtttgccga accaccacct
    18181 ttggcgatac agaggaattt gtactcgtcg ccatcaacgg cataaagatc gatctgcgct
    18241 ggcagattgg tgccggtatt cacctcttta tacatatcca gcggcgcgtt ttgcgagtag
    18301 cgcaaattat cttcgatata agtgttatag acaccgcgcg ccagcgccgc ttcatcacag
    18361 ccaccggtcc atacacgctg ccctttttta ccaacaataa tcgcagtgcc ggtatcctga
    18421 caggttggca gaacgccttt cgccgcgatg tcggagttac gcaggaattg cagcgccaca
    18481 tatttatcat tttcgctggc ctccgggtca cgcagaatgt cggccacctg ttgttggtga
    18541 gccggacgca gcataaacga cgcatcatga aacgcctgac gcgccaacag agttaacgct
    18601 tcgggtgcga ctttcaaaat ctcctgccct tcaaattcag atacgctaac gtgttcgctg
    18661 gttagcaggt aatactcagt atcatctttt ttgagtggaa aaggagcctg ataatgaaag
    18721 ggtttgtttg acattgttct ctcacttact gcctggtttg gttatgctct gggcgggtgt
    18781 tccgttgccc tgttaaaagc gagtaacaat atcctacaca cttttttaac aaaaactgag
    18841 actagtacga ctttttgcgg ctccaggtta cttcccgtag gattcttgct ttaatagtgg
    18901 gattaatttc cacattaaaa cagggattga tcatgcaaaa actcattaac tcagtgcaaa
    18961 actatgcctg gggcagcaaa acggcgttga ctgaacttta tggtatggaa aatccgtcca
    19021 gccagccgat ggccgagctg tggatgggcg cacatccgaa aagcagttca cgagtgcaga
    19081 atgccgccgg agatatcgtt tcactgcgtg atgtgattga gagtgataaa tcgactctgc
    19141 tcggagaggc cgttgccaaa cgctttggcg aactgccttt cctgttcaaa gtattatgcg
    19201 cagcacagcc gctctccatt caggttcatc caaacaaacg caattctgaa atcggttttg
    19261 ccaaagaaaa tgccgcaggt atcccgatgg atgccgccga gcgtaactat aaagatccta
    19321 accacaagcc ggagctggtt tttgcgctga cgcctttcct tgcgatgaac gcgtttcgtg
    19381 agttttccga gattgtctcc ctactccagc cagtcgcagg tgcacatccg gcaattgctc
    19441 actttttaca acagcctaat gccgaacgtt taagcgaact gttcgccagc ctgttgaata
    19501 tgcagggtga agaaaaatcc cgcgcgctgg cgattttaaa atcggcgctc gacagtcagc
    19561 agggtgaacc gtggcaaacg attcgtttaa tttctgaatt ttacccggaa gacagcggcc
    19621 tgttctcccc gctgttactg aatgtggtga aattgaatcc tggcgaagcg atgttcctgt
    19681 tcgctgaaac accgcacgct tacctgcaag gcgtggcgct ggaagtgatg gcgaactccg
    19741 ataacgtgct gcgtgcgggt ctgacgccta agtacattga tattccggaa ctggttgcca
    19801 atgtgaaatt cgaagccaaa ccggctaacc agttgttgac ccagccggtg aaacaaggtg
    19861 cagaactgga cttcccgatt ccagtggatg attttgcctt ctcgctgcat gaccttagtg
    19921 ataaagaaac caccattagc cagcagagtg ccgccatttt gttctgcgtc gaaggcgatg
    19981 caacgttgtg gaaaggttct cagcagttac agcttaaacc gggtgaatca gcgtttattg
    20041 ccgccaacga atcaccggtg actgtcaaag gccacggccg tttagcgcgt gtttacaaca
    20101 agctgtaaga gcttactgaa aaaattaaca tctcttgcta agcttattaa aggcttataa
    20161 caccttcagg cggccagtcc gcctgatttc attttatgga taatcattat gaataaatcg
    20221 ctggtagcgg taggcgtcat tgttgcgcta ggcgtagtct ggacaggcgg cgcatggtat
    20281 acaggcaaga agattgaaac ccatctcgaa gacatggtcg cgcaggcgaa cgcgcaactc
    20341 aaactgacag ctcctgaatc caacctggaa gtgagttatc aaaactatca tcgcggcgta
    20401 ttcagcagcc agttgcaact gttggtgaaa cccattgccg ggaaagaaaa tccgtggatt
    20461 aaaagcggtc agagcgtcat cttcaacgaa tcggttgatc atggtccctt cccgcttgcc
    20521 cagcttaaaa aactgaacct gatcccgtcg atggcatcaa ttcaaaccac gctggttaat
    20581 aacgaagtaa gcaaaccact gtttgatatg gcaaaaggtg aaacgccttt tgagattaac
    20641 tcgcgcattg gttacagcgg tgattccagt tccgatattt cgctcaagcc actgaattac
    20701 gagcaaaagg atgaaaaagt cgcctttagc ggcggcgagt tccagttaaa tgctgacaga
    20761 gacggcaaag ccatctccct ttccggggag gcgcaaagtg gtcggataga cgcagttaac
    20821 gaatacaacc agaaagtgca gttgaccttt aataatctga aaaccgacgg ttccagcacg
    20881 ctggcaagtt ttggtgagcg cgtaggaaac caaaaactgt cactggaaaa aatgaccatt
    20941 tcagtggaag gcaaagaact ggcattgctg gaaggcatgg agatcagcgg taaatcggat
    21001 ctggtcaatg acggtaaaac gatcaatagc caactggatt actcgctaaa cagcctgaag
    21061 gtacagaatc aggatctggg cagcggcaag ctgactttaa aagtcggcca gattgatggt
    21121 gaagcctggc atcagtttag ccagcaatat aacgcgcaaa ctcaggcgct gctggcacag
    21181 ccagaaattg ccaacaatcc cgaactttat caggagaaag tgacggaagc cttctttagc
    21241 gccctgccgc tgatgttgaa aggcgatccg gtgattacta tcgcgccgct aagctggaaa
    21301 aacagtcagg gtgaaagtgc gctgaatctg tcgctgttcc tgaaagatcc ggcaacgact
    21361 aaagaagcgc cgcaaacgct ggcgcaggaa gtagatcgtt cggttaaatc tctggatgcg
    21421 aaactgacca ttccggtgga tatggcaact gagtttatga ctcaggtagc gaagctggaa
    21481 ggttatcagg aagatcaagc gaaaaaactg gcgacacagc aagttgaagg tgcatcagca
    21541 atggggcaga tgttccgtct gaccaccttg caggacaata ccatcaccac cagcctgcaa
    21601 tatactaacg gtcagataac gttaaacggg cagaaaatgc cactggaaga tttcgttggt
    21661 atgtttgcaa tgccggcatt aaatgttccg gtcgtacccg ctattccgca gcagtaattc
    21721 acagacaaaa cgccggagat ttttctctcc ggcgttattt tttacttcag cataaagtca
    21781 tactgaatac tcattttcac atcccagtaa ttcaggcgat cgcccagcgt actgccatcg
    21841 cgcttttccc gccctttatc ggcagcaaga aagaaattca tcccttgtaa cggcccttcg
    21901 ctgacccgat agttcatacc acctaagata cccgtgcctt caatccgcac atctcgttta
    21961 ctggattcat agccgcgagt cacaatcggt gcaaatgtca gcgtaaattg cggcgttaaa
    22021 cgatagttga cgcccaattg ccaggactgc attccttcac ggttacgatc aagacttaat
    22081 gaaaagggat agccaatgtc ggtatccacc acggcaccat tcattgcatg gcgcaaataa
    22141 ccaccactgc caaatacact tccccacggc gcattccagg tcaattgtcc actaaccaac
    22201 gccgtttcat tgggctggct ggtattacga cttaaacctt cgagctgcgc gtaaaagccg
    22261 agcaactccc ctttcaacgt ataattcggg tcgagcggta tgcttttctc gagaatactt
    22321 tgtatctgat tggaaacatc tttctgtacc gatgccccca gagaatagcg actggtatca
    22381 tcatgccaac tggcggctaa aaaagaacgt gcgcgatgtt tatcgtattt tcccgtctgg
    22441 ctgttataca ccccttcatc acgaataccg gttgttgaat cagtccataa aaatactcgt
    22501 gaacgccaca ttccccacag atcaatattt ggtgttaaag cccaataacc taatgctcct
    22561 tcagaaactg tcggtaatgt acgttgcgaa ctgccagcca ttaacggaat acgataaggt
    22621 aagctaccat aactataatc cgtcccgaag cgacctaatt taagcaggct attttcactg
    22681 ggttttattt ttactgcccc cagggcaaag ccaaaacttt tatcaccatc aagataccac
    22741 cgggtactca tatcagcacg agcacctaat ttataaacat aatacgcccc gccttcaacg
    22801 ccaataatat tggaataata accactattg aaatcgagca atcctccctg tacccatgcg
    22861 taaatatctc ttcccgcgcc agcactgggc ttatccgccc tgcgcaattc gtttttcagt
    22921 ctgatgcgta gtcccgcttc atcgtcagcc agttgcgccg cataagcaga ggtaaggcca
    22981 gaggcagccg tcaggcaaat aacggccatg gccactattt ttctc
//
LOCUS       DAPHBJ010000060        22649 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.060, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000060 DAPHBJ010000000
VERSION     DAPHBJ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.060"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..671
                     /locus_tag="RHZ76_002063"
     CDS             <1..671
                     /locus_tag="RHZ76_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2812705.1"
                     /translation="GLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQLSRKVKF
                     SNNWQKQKRKIQRLHSCIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSKSAAGTV
                     SQPGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPAYTSQRCAYCGHT
                     AKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMI
                     QATA"
     gene            complement(711..1925)
                     /gene="ydcO"
                     /locus_tag="RHZ76_002064"
     CDS             complement(711..1925)
                     /gene="ydcO"
                     /locus_tag="RHZ76_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HDV2812706.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            1978..2514
                     /gene="sutR"
                     /locus_tag="RHZ76_002065"
     CDS             1978..2514
                     /gene="sutR"
                     /locus_tag="RHZ76_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HDV2812707.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            2587..4548
                     /gene="rlhA"
                     /locus_tag="RHZ76_002066"
     CDS             2587..4548
                     /gene="rlhA"
                     /locus_tag="RHZ76_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HDV2812708.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(4640..4870)
                     /locus_tag="RHZ76_002067"
     CDS             complement(4640..4870)
                     /locus_tag="RHZ76_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HDV2812709.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            5092..5268
                     /gene="hicA"
                     /locus_tag="RHZ76_002068"
     CDS             5092..5268
                     /gene="hicA"
                     /locus_tag="RHZ76_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HDV2812710.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            5314..5730
                     /gene="hicB"
                     /locus_tag="RHZ76_002069"
     CDS             5314..5730
                     /gene="hicB"
                     /locus_tag="RHZ76_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HDV2812711.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5809..7215
                     /locus_tag="RHZ76_002070"
     CDS             5809..7215
                     /locus_tag="RHZ76_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDV2812712.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            7460..8605
                     /locus_tag="RHZ76_002071"
     CDS             7460..8605
                     /locus_tag="RHZ76_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV2812713.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            8623..9636
                     /locus_tag="RHZ76_002072"
     CDS             8623..9636
                     /locus_tag="RHZ76_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2812714.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDPLMAENLCGMTGSFALRPEHIRLNIPGEMQANGT
                     IQAVQYQGAATRFELTLSGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            9637..10578
                     /locus_tag="RHZ76_002073"
     CDS             9637..10578
                     /locus_tag="RHZ76_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2812715.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIILIALYLAFVKRLGAFDAL"
     gene            10568..11362
                     /locus_tag="RHZ76_002074"
     CDS             10568..11362
                     /locus_tag="RHZ76_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2812716.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            11384..12808
                     /gene="patD"
                     /locus_tag="RHZ76_002075"
     CDS             11384..12808
                     /gene="patD"
                     /locus_tag="RHZ76_002075"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HDV2812717.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(12905..13000)
                     /gene="yncL"
                     /locus_tag="RHZ76_002076"
     CDS             complement(12905..13000)
                     /gene="yncL"
                     /locus_tag="RHZ76_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HDV2812718.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            13195..13368
                     /gene="ortT"
                     /locus_tag="RHZ76_002077"
     CDS             13195..13368
                     /gene="ortT"
                     /locus_tag="RHZ76_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HDV2812719.1"
                     /translation="MSLYQHMLVFYAVMAAISFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            13454..13687
                     /locus_tag="RHZ76_002078"
     CDS             13454..13687
                     /locus_tag="RHZ76_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HDV2812720.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(13688..14137)
                     /locus_tag="RHZ76_002079"
     CDS             complement(13688..14137)
                     /locus_tag="RHZ76_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV2812721.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(14134..14652)
                     /gene="mddA"
                     /locus_tag="RHZ76_002080"
     CDS             complement(14134..14652)
                     /gene="mddA"
                     /locus_tag="RHZ76_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HDV2812722.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTLAGYPVLVSEENGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            14833..15870
                     /gene="curA"
                     /locus_tag="RHZ76_002081"
     CDS             14833..15870
                     /gene="curA"
                     /locus_tag="RHZ76_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HDV2812723.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            16068..16733
                     /gene="mcbR"
                     /locus_tag="RHZ76_002082"
     CDS             16068..16733
                     /gene="mcbR"
                     /locus_tag="RHZ76_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HDV2812724.1"
                     /translation="MPGTGKMKHFSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(16769..18871)
                     /locus_tag="RHZ76_002083"
     CDS             complement(16769..18871)
                     /locus_tag="RHZ76_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDV2812725.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTSVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYITPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLIGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGVNIAWRFE"
     gene            19113..20174
                     /locus_tag="RHZ76_002084"
     CDS             19113..20174
                     /locus_tag="RHZ76_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDV2812726.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(20289..21788)
                     /gene="ansP"
                     /locus_tag="RHZ76_002085"
     CDS             complement(20289..21788)
                     /gene="ansP"
                     /locus_tag="RHZ76_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HDV2812727.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLSSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            22054..>22649
                     /locus_tag="RHZ76_002086"
     CDS             22054..>22649
                     /locus_tag="RHZ76_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HDV2812728.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIAFMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEIWLNSQLTAEPYAFGEQLTLVDCYLCTMR
                     TWGPGHEWFQDNAQNISAIADAVCQLPKLQEVLKRNEI"
BASE COUNT         5751 a         5587 c         5875 g         5436 t
ORIGIN      
        1 tcgggctgga tgctggcgtg gctaaactcg ccacgctgtc agatggcaca gtctttgagc
       61 ctgtaaacag ttttcagaaa aaccagaaga agctggcgag acttcagcga cagttaagcc
      121 gcaaggtcaa attcagcaac aactggcaaa agcagaaacg caaaatacag cgactgcatt
      181 cctgtatcgc aaatatccgc agggactacc ttcacaaagt cacaacggcc gtcagcaaaa
      241 accacgcaat gatagtcatt gaggatttga aggtcagcaa catgtcaaag tcagcagcgg
      301 gtacggtcag ccagccgggg cgcaatgtcc gggcaaaatc aggtttaaac cgttcgatac
      361 tggatcaggg ctggtatgaa atgcgccgcc agcttgcgta caagcagctc tggcgtggtg
      421 gtcaggtgct tgctgttccg ccagcgtata caagccagcg ttgcgcgtac tgtggtcata
      481 cagcgaaaga gaaccgcctg tcacaaagta aattcagatg ccaggtatgt ggatatacag
      541 cgaacgccga tgtaaatggc gctcgcaaca ttttagcggc ggggcacgcc gttcttgcct
      601 gtggagagat ggtgcagtca ggccgcccgt tgaagcagga acccaccgaa atgattcagg
      661 cgacagcctg aacgtagcag ggatccacgt ccttcagggc gtggaggatg tcaatatcgg
      721 tttctgtcag cgattaaatt caacaccacg taacaaacgc ctccggcaat taatccccaa
      781 aacgcagaac caatcccgac cagcgtcaat ccacttgccg ttaccagaaa tgccaccacc
      841 gccgcgtctc gctcacgctc attatgcagc gcctgataca aactgccgcc gatggtactt
      901 aacagcgcca gacctgccag catctggatc caacttacgg gcagggcagc catcatcccg
      961 gtaatggcac taccaaacag acctgcgagc aaatagaaaa tgcctgcaac ggcagcggcc
     1021 agccaacgtt gatctttatc cggatgcgct tccgggcttt ggcaaatagc cgcggtgatt
     1081 gccgcaatac cgacggaata aacgccgaaa ggggaaaaaa ccagtgccag caatccagta
     1141 aatacaatta atggcgaaac aggagccgaa tatccagctg ctttcattgc tgcgataccc
     1201 ggtgcgtttt gcgatgccat cgtcaccaga aaaaggggga gtgcaacgct caggctgtga
     1261 gcaaacgaaa aatcaggggt aatataagtg gggagaacgg gtttaaagac aacatcagtt
     1321 gtgacaacgt caccttgcgc gatgacgatc acgatcccaa taatcatcgc ggcaattacc
     1381 gcatagcgcg gcgcaacggc cttggttgcc agccatacca gcaacatact tccacacaac
     1441 gtaaattgac cgtccagact ggcaaacgcc tgtaaaccaa agcgtaataa aatcccggca
     1501 agcattgccg ccgcaagcga gtgcggaata atgcgcatca gacgagcaaa gagtcccgtt
     1561 atgccgcaga ggactattag cgcgttggtg acaataaaaa cgccgatggc ttcgttaagt
     1621 gttagtccct gcaatccggt gaccaacaaa gccgcgccag gcgttgacca tgcggtgaga
     1681 acaggtacgc gataccataa tgtcagagtc agcgtactga cgcccattgc cagccccagc
     1741 gccgtcatcc agccagagat ttgtgcagtg gtggctccgg cgacaatcgc tgcttgccag
     1801 attattgccg ctgaactggc gtagccaatt aataccgcca gaaaccccgc cagtagcgtg
     1861 ggtggaggaa tagaaaacag acgcatagtc acctcgtgcg ttatagcgta caggcaccgt
     1921 agcatttgtc cgttataacg cacaagtgat aaacttccgt tttgccggag gagtcgcatg
     1981 gaaaatctcg ctcgcttttt atccaccaca cttaaacaac tacgccagca gcgcggctgg
     2041 agtctttcgc gactggcaga agcgacgggc gtttctaaag caatgttagg gcagattgag
     2101 cgtaatgagt ccagtccgac ggtcgcgaca ttatggaaaa ttgccaccgg gttgaacgta
     2161 ccattttcaa catttatttc tccgccgcag tccgctacgc cttcagttta tgatccacaa
     2221 cagcaggcga tggtgatcac ttcgctgttt ccttatgatc cacaactctg ctttgaacac
     2281 ttctcgattc agatggcatc tggtgcaata agcgaatcga cgccccatga gaagggggta
     2341 atcgaacatg tcgtcgtcat tgatggacaa cttgatctgt gcgttgatgg ggaatggcag
     2401 acccttaatt gtggggaagg cgttcgattt gctgcagacg tcacgcatat ctatcgtaat
     2461 ggtggggagc aaaccgtaca ttttcattcc ctcatccatt acccgcgcag ttaagcggga
     2521 aaactatttc gcaacgtcgc gcttgtggct aaaatagccg ccatttttca gctactggat
     2581 aagaatgtga ccgtatcttc tcatcgactt gaactgttaa gcccggcacg cgatgccgcc
     2641 attgcccgcg aagctatttt gcacggtgcc gatgctgttt atatcggcgg ccctggtttt
     2701 ggtgcccgtc ataatgccag taatagcttg aaagatattg ccgagctggt gccgtttgcc
     2761 catcgttatg gtgcaaaaat tttcgtcacg cttaacacca ttttgcatga tgatgagctg
     2821 gaacccgcgc aacggctgat tactgacctc taccagaccg gtgtcgatgc gctgattgtt
     2881 caggatatgg ggattctgga acttgatatt ccgccgattg aactgcacgc cagtacgcag
     2941 tgcgacattc gtacagttga aaaagcgaag ttcctctctg atgttggctt cacgcagatt
     3001 gtgctggcgc gagagctgaa tcttgatcag atccgcgcga ttcaccaggc tacggacgcg
     3061 accattgaat tctttattca tggggcactg tgcgtggcct attcgggtca gtgctacatt
     3121 tctcatgcgc aaacagggcg tagcgccaac cgtggcgatt gctcgcaggc gtgccgtttg
     3181 ccatacacat tgaaagacga tcaggggcgg gtggtttcct atgaaaaaca tctgctgtcg
     3241 atgaaagata acgatcagac tgccaacctc ggcgcgctga ttgatgctgg tgtacgctcc
     3301 ttcaagattg aagggcgtta caaagatatg agctacgtga agaatatcac cgcccattat
     3361 cgccagatgc ttgatgccat tattgaagaa cgtggcgatc tggcgcgcgc ttcatcaggt
     3421 cgtactgaac atttctttgt tccatcgacg gaaaagactt tccaccgtgg tagcacagat
     3481 tattttgtga atgcccgtaa aggcgatatt ggcgcgttcg attcgccgaa atttatcggc
     3541 ctgccggtag gcgaagtatt gaaagtggcg aaagatcatc tcgatgttgc cgttaccgag
     3601 ccactggcaa atggcgatgg cctgaacgtg ttgattaaac gtgaagtcgt cggttttcgt
     3661 gccaatacgg tcgagaaaac cggagaaaat cagtaccgcg tctggcccaa tgaaatgcca
     3721 gcagatttgc acaaaattcg tccacatcac ccactaaacc gtaatcttga tcataactgg
     3781 cagcaggcac tgacaaaaac ctccagcgaa cgtcgggtgg cggtagacat tgaactgggc
     3841 ggctggcagg aacaactgat tctgaccctc accagtgaag agggtgtcag catcacgcat
     3901 acgctggacg ggcagttcga cgaagccaat aacgccgaaa aagcaatgaa caatctgaag
     3961 gatggtctgg caaaactggg gcaaaccctc tattacgccc gcgatgtgca aattaatttg
     4021 ccgggggcgc tgtttgtacc aaacagtctg ttaaaccagt tccgccgtga agctgctgac
     4081 atgctggatg ctgcgcgtct tgccagttac cagcgcggca gccgtaaacc ggttgctgat
     4141 cctgcgccgg tttatccgca aacgcatctg agtttcctcg cgaacgtata caaccagaaa
     4201 gcgcgtgaat tttatcatcg ctatggtgtg cagctgattg acgcggcgta tgaagcacat
     4261 gaagagaagg gcgaagtccc ggtgatgatc accaagcatt gtctgcgctt tgcctttaat
     4321 ctgtgcccga aacaggcgaa aggcaatatc aaaagctgga aggcgacgcc aatgcaactg
     4381 gttaacggcg atgaagtatt aacgctaaag tttgattgcc gcccatgcga gatgcacgtc
     4441 attggcaaaa tcaaaaatca catactgaaa atgccgttac cgggaagcgt agtggcatcc
     4501 gtaagtccgg atgagctgct gaaaacattg cctaagcgaa aagggtaaaa cgccagtttt
     4561 ctggttactc acaactcatt gaatctgcat gatattgcct gccgggtaag gcgtcacgcc
     4621 gcatccggca acaaatgact caacgttgtc ccgcttctgg tttgcgcgat ttttgccagt
     4681 aaaaatgctc gcgcaaacct tccgccgact cttccgccac agcacgcaat tcatcgctgt
     4741 ccgcttcatg acgcagctga tgatccacat tctttaccca cacaaattca tgtcctttgt
     4801 gccctgccat gagttgtcct gagaacagcg cacacgttaa taagacaacc gataacgcct
     4861 tcgtaaacat cctgccacct ttttcttacc ttttgccgct atgatgccga tcgtttcttg
     4921 aggttattat tcagttttgc aaattagcgc aaagaaattc tggaatcttc cttcctgatt
     4981 ttgcatggca ttctgccgtt gcggcgattt agtgctattt tcgagcaaat tacacacgga
     5041 ggtaaacact aatgtttact ttgttgatat actcagcggc agggaggcga tgtgaaacaa
     5101 agcgagttca gacgttggct cgaatctcag ggcgtcgatg tagcgaatgg cagcaaccat
     5161 ttgaaactca ggtttcatgg gaggcgcagt gtcatgccgc gtcacccctg cgatgagatt
     5221 aaagaaccat tgcgtaaagc aatcctgaaa caactcggtt tgagttaatc gccaattaaa
     5281 aaggttaatg acatgcgagg gacagtcgaa attatgcgtt atcccgtcac tcttacaccc
     5341 gcgccggaag gcggttatat ggtttctttt gtggatatcc ctgaagcgtt gacgcagggc
     5401 gaaactgtcg ctgaagcgat ggaagcggca aaagatgctt tactgaccgc atttgatttt
     5461 tattttgaag ataacgagct tatcccttta ccttcgccat taaatagtca tgatcacttt
     5521 attgaagtgc ctttgagcgt cgcctctaag gtattgctgt taaatgcttt tttacagtca
     5581 gaaatcactc agcaagagtt agccaggcga attggcaaac ctaaacagga gattactcgc
     5641 ctatttaact tgcatcatgc gacaaaaatc gacgccgtcc agctcgcggc aaaggcgctt
     5701 ggcaaagagt tatcgctggt gatggtttaa ttacagttaa cgaaaagttg tcatttttaa
     5761 caactgatat agactgccga atcatctgca cataattacg attcgataat gaaaaaatac
     5821 cagcagcttg cagaacaatt acgcgagcag attgcgtcgg gtatctggca acccggcgat
     5881 cgtttgcctt cgttgcgtga ccaggtggcg ctttcaggca tgagctttat gactgtcagc
     5941 catgcctatc agttgctcga aagtcaggga tatattatcg cacgaccgca gtcgggttat
     6001 tacgttgcgc cacaggcaat aaaaatgccg aaagcgccag tcattccagt cactcgagat
     6061 gaagcagtcg atatcaacac ttatattttt gatatgttgc aggccagtcg cgatccgtcg
     6121 gtcgttccgt ttgcctcggc ctttcccgac ccgcgacttt tccccctcca acaactaaac
     6181 cgctcgctgg cgcaggtaag caaaaccgcc acagcgatga gcgtgattga aaacttaccg
     6241 ccaggaaacg cagaactgcg tcaggctatt gctcgtcgct atgccttaca gggcatcacc
     6301 atttctcctg atgaaattgt cattactgcc ggggcgttag aggcattaaa cctcagtttg
     6361 caagcggtaa ctgaaccggg cgattgggtg atagtagaga atccttgttt ctacggtgcg
     6421 ttgcaggcgc tggagcggct acggctgaag gcgttatcgg tggcgacgga tgttaaagaa
     6481 gggatagatc ttcaggcgct ggaactggcg ttgcaggagt atccggtgaa agcgtgctgg
     6541 ctgatgacta atagccagaa tccactcgga tttaccttaa cgccgcaaaa aaaagcacaa
     6601 ctggtggcgt tgctcaatca gtacaacgta acgctgattg aagatgacgt ttacagcgaa
     6661 ctttattttg gacgggaaaa accgctgcct gcgaaagcgt gggatcgcca cgatggcgtt
     6721 ttgcattgct cttcgttttc gaaatgtctg gtgcctggtt ttcgtattgg ttgggtcgcc
     6781 gccggaaaac atgcacgtaa aattcaacgc ttgcagttga tgagtacgct ttccaccagc
     6841 tcaccgatgc aacttgcgct ggtggattac ctttccacgc gccgatacga cgcccatctt
     6901 cgtcgcctgc gtcgccagct tgcggaacgt aaacaacgtg cctggcaggc actgctgcgt
     6961 tatctgcctg cggaagtgaa aattcatcat aatgacagtg gttactttct ctggctggag
     7021 ctccccgagc cgttagatgc cggcgaatta agcctggcgg cactgacgca tcatatcagt
     7081 attgcgccgg gtaaaatgtt ttctaccggt gaaaactggt cacgtttttt ccgttttaat
     7141 accgcgtggc agtggggaga gcgtgaagaa caggcggtaa aacaattagg caaacttatt
     7201 caagaacggc tgtaatagcg tttaatttaa ttcctcttag attgggtaat atgaatttcg
     7261 aatagcagtc atatttccta actccttgac tatactccag aagataacct tacagacggc
     7321 ataatgcgcg gtagctcaca acctgattaa attttctcag gggcgaaggt gtgcctgcaa
     7381 gccgccgtct atggttaaac aaggagatat ttttacggca cggcggctga acaattaatt
     7441 acgacaggag taagacctta tgagcaagac atttgcccgc agcagcctgt gtgcgctcag
     7501 catgacaata atgaccgctc acgccgccga accgcctacc aatttagata aaccggaagg
     7561 gcgactggat attatcgcct ggccgggata catcgaacgc ggacaaactg ataaacaata
     7621 cgactgggta acgcagttcg aaaaagagac aggctgcgcg gtgaatgtga aaaccgccgc
     7681 gacttccgat gaaatggtca gtctgatgac caaagggggt tacgatctgg ttacggcatc
     7741 cggcgatgcc tcgctgcgtt tgattatggg taaacgcgtg cagccgatta ataccgcatt
     7801 gattcccaac tggaaaacgc tcgatccgcg cgtggttaaa ggcgactggt ttaatgttgg
     7861 cggcaaagtt tacggcacac cttaccaatg ggggccgaac ctgctgatgt acaacactaa
     7921 aaccttcccg acgccgccgg atagctggca agtggttttt gttgagcaaa atctgccgga
     7981 cggcaagagc aataaaggcc gcgttcaggc ttatgatggc cctatctaca ttgcggacgc
     8041 tgcgttgttc gttaaagcca ctcagccgca gttgggcatc agcgatccgt atcaactcac
     8101 cgaagaacag taccaggcgg tgctgaaagt gctgcgcgct caacacagtt tgatccatcg
     8161 ctactggcat gacactaccg tgcaaatgag cgatttcaaa aacgagggtg tggttgcttc
     8221 cagtgcctgg ccctatcagg ccaacgccct gaaagccgaa ggccagcctg ttgctaccgt
     8281 tttcccgaag gagggtgtta ccggttgggc tgataccacc atgctgcata gcgaagcgaa
     8341 acatccggtt tgcgcctaca aatggatgaa ctggtcatta acgccaaaag tgcagggcga
     8401 tgtggcggcc tggtttggct cgttaccggt agtgccggaa gggtgtaaag ccagtccgtt
     8461 attaggcgaa aaaggttgtg aaaccaacgg ttttaactat ttcgacaaaa tcgccttctg
     8521 gaaaacgcct atagcagaag ggggcaagtt tgttccctac agtcgctgga cgcaggatta
     8581 cattgccatt atgggcggtc gctaacttcg ctggggtgct ttatgacgta cgcagtggag
     8641 tttgacaacg tctcgcggtt gtacggtgac gtgcgcgcag tagatggcgt cagtattgcg
     8701 ataaaagatg gtgagttctt ctctatgctg gggccgtccg gctccggcaa aaccacctgc
     8761 ctgcgcctga ttgctggctt cgaacagctt tccggcgggg ctatctctat ctttggtaaa
     8821 cccgccagca atctgccacc gtgggagcgg gacgtgaata ctgtctttca ggactacgcg
     8881 ctatttccgc atatgtcgat tcttgacaat gtcgcctatg ggctgatggt caaaggcgtg
     8941 aataaaaagc agcggcacgc aatggcgcaa gaggcgctgg agaaagtggc gttggggttt
     9001 gtacatcaac gtaaaccgtc acaactttct ggtggtcagc gccagcgggt tgctatcgcc
     9061 agagcattgg tgaatgaacc gcgcgtattg ctgttggatg aaccgctcgg cgcactggat
     9121 ctcaaattgc gtgagcagat gcagctggaa ctgaaaaaac tgcaacagtc tctcggtatc
     9181 acttttatct tcgttaccca cgatcagggc gaagcgttat cgatgtccga tcgtgtggcg
     9241 gttttcaata atggacgcat tgagcaggtc gattccccgc gcgatctcta tatgcgcccg
     9301 cgcacgccgt ttgttgccgg attcgtcggg acatccaacg tttttgatcc gctaatggcc
     9361 gagaatcttt gcggcatgac gggaagcttc gccctgcgac cggaacatat ccgtctcaat
     9421 atccccggcg aaatgcaggc taatggcacg atccaggcgg tgcaatatca gggcgcagcg
     9481 acccgttttg aactgacact gagcggcggt gaaaaactgc tggtgagtca ggccaatatg
     9541 acaggcgaag aactgcctgc cacgctcacg cccggacaac aggtgatggt ttcctggtcg
     9601 cgtgatgtga tggtgccgct ggttgaggag aggtgaatgg cgatgaatgt attgcaatca
     9661 ccttcacgtc caggtctggg taaggtgtcc ggtttcttct ggcgtaatcc ggggctgggg
     9721 ctgtttttac tgctgcttgg cccgctaatg tggtttggca ttgtctattt cggctcgctg
     9781 ctgacactgt tatggcaggg attttatact tttgacgatt tcaccatgtc ggtaacgccg
     9841 gaactgacgc tggcgaatat ccgtgcgctg tttaatccgg cgaattacga catcattctc
     9901 cgcacgctga ctatggctgt ggcagtcact atcgccagcg ccattctggc ttttccaatg
     9961 gcgtggtata tggcgcgcta taccagcggg aaaatgaaag cgttttttta tattgcggta
    10021 atgttgccga tgtgggcgag ctacattgtt aaagcctatg cctggacgtt attgctggca
    10081 aaagatggcg tggctcagtg gtttttacaa catcttgggc tggaaccact gctgactgcg
    10141 ttccttacat tacctgcggt gggcggaaat acgctgtcaa cttccgggct ggggcgcttt
    10201 ctggtgtttc tctatatctg gttgccgttc atgatcctgc ccgttcaggc ggcgcttgag
    10261 cgtttgccgc cgtcattgtt gcaggcgtcg gctgatctcg gcgcacgtcc acgacaaacc
    10321 tttcgctatg tggtgctgcc gctggcaatc ccgggtattg ccgctggctc tatctttacc
    10381 ttctcactca cactgggcga ttttatcgtc ccgcagctgg ttggtcctcc aggatatttt
    10441 atcggcaata tggtttattc ccagcagggg gcgattggca atatgccgat ggcggcggca
    10501 ttcaccctgg tgccgattat tctcatcgca ctgtacctgg cgttcgtgaa acgtctggga
    10561 gcgttcgatg cactctgaac gcgcaccgtt tttcctcaaa ctggcggcct ggggcggcgt
    10621 tgttttccta cattttccca tcctgataat cgccgcctat gcgtttaaca ctgaagatgc
    10681 ggcgtttagt tttccaccgc agggcctgac gctgcgctgg tttagcgtgg cagcacagcg
    10741 tagtgatatt cttgatgccg tgacactgtc acttaaagtg gcggcgctgg cgacattaat
    10801 tgcgctggtg ttagggacgc tggcagctgc cgcgctgtgg cgacgagact ttttcggcaa
    10861 aaacgccatt tcgctgttac tgctgctgcc cattgcgctg ccgggcattg tcactggtct
    10921 ggcgttatta accgccttta aaaccatcaa tctggagccg ggatttttca ccatcgtggt
    10981 cggtcatgcg actttttgtg tagttgtggt gtttaacaat gtcatcgccc gttttcgccg
    11041 cacctcctgg agtctggttg aggcgtcaat ggatcttggg gccaatggct ggcaaacctt
    11101 ccgctacgta gtgttgccga atctcagttc ggcgttactg gcaggaggaa tgctggcgtt
    11161 tgccttgtcg ttcgatgaaa tcatcgttac gacctttacg gcaggtcatg aacgaacgtt
    11221 accgttgtgg ttgctcaatc agcttgggcg accgcgtgat gtaccggtaa ctaacgtggt
    11281 ggcactgctg gttatgttgg taacaacctt gccgatcctg ggggcctggt ggctaacccg
    11341 cgaaggcgac aatggtcaat aaccactgat acaggaatat gctatgcaac ataagttact
    11401 gattaacgga gaactggtta gcggcgaagg ggaaaaacag cctgtctata atccggcaac
    11461 gggggacgtt ttactggaaa ttgccgaggc atccgcagag caggtcgatg ctgctgtgcg
    11521 cgcggcagat gcagcatttg ccgaatgggg gcaaaccacg ccgaaagtgc gtgcggaatg
    11581 tctgctgaaa ctggctgatg ttatcgaaga aaatggtcag gtttttgccg aactggagtc
    11641 ccgtaattgt ggcaaaccgc tgcatagtgc gttcaatgat gaaatcccgg cgattgtcga
    11701 tgtttttcgc tttttcgcgg gtgcggcgcg ctgtctgaat ggtctggcgg caggtgaata
    11761 tcttgaaggt catacttcga tgatccgtcg cgatccgttg ggggtcgtgg cttctatcgc
    11821 accgtggaat tatccgctga tgatggccgc gtggaaactt gctccggcgc tggcggcagg
    11881 gaactgcgta gtgcttaaac catcagaaat taccccgctg accgcgttga agttggcaga
    11941 gctggcgaaa gatatcttcc cggcaggcgt gattaacata ctgtttggca gaggcaaaac
    12001 ggtgggtgat ccgctgaccg gtcatcccaa agtgcggatg gtgtcgctga cgggctctat
    12061 cgccaccggc gagcacatca tcagccatac cgcgtcgtcc attaagcgta ctcatatgga
    12121 acttggtggc aaagcgccag tgattgtttt tgatgatgcg gatattgaag cagtggtcga
    12181 aggtgtacgt acatttggct attacaatgc tggacaggat tgtactgcgg cttgtcggat
    12241 ctacgcgcaa aaaggcattt acgatacgct ggtggaaaaa ctgggtgctg cggtggcaac
    12301 gttaaaatct ggtgcgccag atgacgagtc tacggagctt ggacctttaa gctcgctggc
    12361 gcatctcgaa cgcgtcagca aggcagtaga agaggcgaaa gcgacagggc acatcaaagt
    12421 gatcactggc ggtgaaaagc gcaagggtaa tggctattac tatgcgccga cgctgctggc
    12481 tggcgcatta caggacgatg ccatcgtgca aaaagaggta tttggtccag tagtgagtgt
    12541 tacgcccttc gacaacgaag aacaggtggt gaactgggcg aatgacagcc agtacggact
    12601 tgcatcttcg gtatggacga aagatgtggg cagggcgcat cgcgtcagcg cacggctgca
    12661 atatggttgt acctgggtca atacccattt catgctggta agtgaaatgc cgcacggtgg
    12721 gcagaaactt tctggttacg gcaaggatat gtcactttat gggctggagg attacaccgt
    12781 cgtccgccac gtcatggtta aacattaagg ataatattgc agatcgtaag agtatgagat
    12841 gatcttgcgt actgtcagag cactgatttc tctgacagta cgtgctgcgt gcaaaatcac
    12901 tacatcaaat aaaccagcca aatctcatag agataagagt aaacaaacca acagcagcaa
    12961 agacatttat cagtactaca gttctactgg aaacattcat ttttgccacc ttttatcatt
    13021 gcccctatgt gtatagtacg ggttaagaaa atccgcaata atactcattc tgaagatgga
    13081 taagggcaag ttgctgtttg atgatttttt ctcaaactgg tgaaaaaact tgatgcacgt
    13141 caaaaaatga cgcatatttg cgcgcgtttt attcatctgg ctggacgccc gtacatgtct
    13201 ctctatcaac atatgcttgt tttttatgcg gttatggcag caatctcatt tcttatcacc
    13261 tggtttcttt ctcacgataa gaaacgcatc cgtttcttaa gcgcctttct ggtgggggca
    13321 acatggccga tgagtttccc ggtggcgctg ttgttttcac tgttttaaat cttactcact
    13381 ggcttgacgg caccacgcag acttatatca tttggatgaa tcgataaatt tcacaagtgg
    13441 ctaaggagaa agtatgtcgc atctggatga agtcatcgcg cgcgtggatg ccgcgattga
    13501 agagagcgtc attgcccata tgaacgaatt attaatcgcc ctgagcgatg acgcggagtt
    13561 aagtcgggaa gatcgctaca cccagcagca acgtctgcgc acagcgatcg cccatcacgg
    13621 tcgcaagcat aaagaagata tggaagcgcg ccatgaacag ttaaccaaag gcggcaccat
    13681 cctgtgatta aaatgagcgt ctggctacca accaggcgcc aaccacaagt aaaattgcgc
    13741 cacaaattgg gccgaacaac gcccgtagtg gtacgccatg actgcggaag atatccagaa
    13801 ccagaccgcc aatcagctga ctggcgacca gtactgcaat agttgtcgcc gctcccacat
    13861 tctgatatcc gctgatactg gcgaagacaa aaaacgatcc cagcaagcca ggtataagtg
    13921 tccaccagcg cacgctggac accagttcgc caaaccccgc catgccttgt ttaaaccata
    13981 aaatagaaac aaagagcaca atccccacca gtgagttcag caacatggcg atgagaatgg
    14041 tagaggaggt ctgggtgatg cgcaccatca gcgtgttctg gacaaccaga ccaattccgg
    14101 cggcaatcaa aaaagcaagg gtgagcgact gattcatcca atcgcgtccg gttcagtgcg
    14161 ctcgtcgagt tgcaactgca taaatgtcag atccagccaa cgaccaaatt tagtgcctac
    14221 ctgcggcatt tgcgcggtga cgacaaatcc cagcgactgg tggagatgca gcgaggcctg
    14281 attttgcgat tcgatcccgg cgaccatgac atgcttcccg caatcccgcg cttcatcaat
    14341 caatcggctt aacaatttac gccccagacc tttgccctga tgatcgggat ggacataaac
    14401 cgaatgttcc acggtatggc gaaaaccatc gaaactacgc cagtcgccaa acgaggcata
    14461 tcccgtcact acgccgtttt cctcgctcac cagcactgga taacctgcta aagtccgcgc
    14521 ttcaaaccag gcaatgcggt tatcagcatc caccgtttgg tcattccaga tagccgccgt
    14581 atacaacacg gcgtggttat aaatttccgc aatggcagca cagtcggctt tgcgggcaaa
    14641 acggatggac atgtctggcc tcgataaaat aatgatgatg atgttatcaa tactatcgaa
    14701 aacagcccga agcaacggat tcctcgatga aattattgcg tgttgcagag gggatgtgac
    14761 ggctgcaaac aaaaccaatc ccgtgagcct gctactattg atggattcat gccaaaagca
    14821 caggagagca ttatggggca acaaaagcag cgtaatcgac gttgggttct ggcctcgcgt
    14881 ccacatggcg caccggttcc ggagaatttc cgtcttgaag aagatgatgt cgccacaccg
    14941 ggtgaaggac aggtgttact gcgcacagtt tatttgtccc tggacccgta tatgcgtgga
    15001 cgtatgagcg atgagccatc ttattcaccg cctgttgata ttggcggcgt gatggtcggc
    15061 ggcacggtga gccgtgttgt ggagtcgaat catcctgatt atcagcctgg cgactgggtg
    15121 ctgggctaca gtggatggca ggactatgac atatccagtg gtgatgatct ggtgaaactt
    15181 ggcgatcatc cgcaaaatcc atcgtggtcg ctgggtgtgc tggggatgcc aggctttacc
    15241 gcttatatgg ggctgctgga tatcggtcag cctaaagagg gcgaaacgtt ggtggtagct
    15301 gcggcgacag gtcctgtggg agcaacggtg gggcaaatcg gcaaacttaa aggttgcagg
    15361 gtggtggggg ttgccggtgg cgcggaaaaa tgccgccatg ctatcgaggt gctgggcttc
    15421 gatgtttgtc ttgatcacca cgcggatgat tttgccgaac aactggcgaa agcgtgccca
    15481 aaaggtattg atatctatta tgaaaacgtg ggcggtaagg tatttgatgc agtgctacca
    15541 ttacttaata catctgcgcg cattcccgtt tgtggcttag tgagcagcta taacgctaca
    15601 gagctaccac ccggtccgga tcgtttaccc ctgttgatgg caacggtgct gaaaaaacgt
    15661 attcgcttgc aagggtttat tatcgctcag gattatggtc accgcatcca tgagtttcag
    15721 aaggagatgg ggcaatgggt gaaagaggat aaaatccact accgcgaaga tattactgat
    15781 ggtttagaga atgcgccaca gacgtttatc ggcctgctga agggtaaaaa cttcggcaaa
    15841 gtggtgatcc gcgtggcggg tgatgattaa agtttcaacg gcggcgtaag ccgccataaa
    15901 aatccaggca aaaagcttat tttcagcttt aattaactaa ccacgtcatt gttctgctgc
    15961 taataacaac aaaggtgagt ggtttatgat tattcacttt aatacaccag gtgaactcct
    16021 tctgccatgc aggcagggtt ggacagaaaa cacgcgaagg aattaaaatg cctggaacgg
    16081 gaaaaatgaa acatttcagt ttgactctgc aggttgagaa cgacctgaaa catcagctta
    16141 gtattggcgc attaaaacct ggcgcacgcc tgattactaa aaatctggcg gagcaattag
    16201 gtatgagtat tacacctgtg cgtgaagcat tattacgtct ggtttcggtg aatgcgcttt
    16261 ctgtcgcacc tgcacaagca tttacagttc cggaagtggg gaaacgtcaa ttagacgaaa
    16321 tcaatcggat ccgctacgaa ctggaattaa tggcagttgc tctagctgtt gaaaacctca
    16381 ccccgcaaga ccttgcggaa ctccaggaat tgctcgagaa attacagcag gcgcaagaaa
    16441 agggcgacat ggaacaaatc attaatgtaa acaggctatt tcgcttagcg atttatcatc
    16501 gctcaaatat gcccatcctg tgtgagatga ttgagcaact gtgggtcagg atggggcctg
    16561 gtttacatta tctctatgaa gcgattaatc cagcggaatt acgggagcat atagaaaact
    16621 atcatctgtt actcgctgcg ttaaaagcaa aagacaaaga gggatgcaga cattgtcttg
    16681 ctgaaattat gcaacaaaat attgctattt tataccagca atacaatcgt taaagtaaat
    16741 gcccggcaaa taccgggcat caaggtaatt actcaaatcg ccatgcgata ttcacgccga
    16801 caccataatt tcgcccgggc gcaggttcgt aatatcgccc gtttgactca ttgacaatga
    16861 cagaaccaac gtattcttta tcgaataaat tatcgacacg accaaataaa tccacagtta
    16921 aattgtggta attgtattta tacccggtga ataagccgat gagagtataa gacggcgcct
    16981 tggccgtatt ttcatcatct gccataatat cgcccatata acgcgcttcc gtgcctgcat
    17041 accagccatc ttccggtaca taacctatcg acgcaaagcc catattacgg gcgatcccag
    17101 gcatccgatt accgttacaa tcctgttcat tgcaaacatt gctgcgatag gtcgcatcaa
    17161 gccaggtcca ggacgcgttt acgcgaaaat ctcccgcgaa acgttgatcc catgccagtt
    17221 cagcgccttg acgacgggtc tttccagcat ttttgtaagt cgtacgccca ccgctactgc
    17281 tatcgacaac aatttcatca tcggtgtcgg tctggaacaa tgcgagacta agcagcccat
    17341 caccaatacg cgttttactg ccgatctcaa ttgtatcgtt ggtggatggt tttaaaccaa
    17401 agttcatacc gctttgccca tcagcccgat aagacagctc attaatcgtc ggcgtttcaa
    17461 aaccacgccc ggctgccaga tagatattcc aggcatcggt cattgcgtat tttaacgatc
    17521 cggctggtag ccatttgtga taactggcat caccgctgtc atcgccgtta cccggagtaa
    17581 tgtaatggtc gttggaatca aaccacacgg agctgtagcg cacgccagca tccagcgaca
    17641 gtttttcgct cagctgccac tgcgtctgta aatagggatc gatgttccac atcagattgc
    17701 gttcgtcgcg acgcaactca cctttttgcc cgtactccgg catgccgcta ttcaggcgga
    17761 agttattgta gcccttgcgg ttttcactca tgttttcgta gttcaggccg gtagtgaacg
    17821 tgaccggaac gcccagttca ccacggtgtg tccagcggct gtctattccc tggtaatggc
    17881 gttgcagggt aatcacgccg cccgcatgtg acgggttaag ttgtggtgcc atgggtattg
    17941 actggtactg ggtcgtttct cgctctccgg catacatcat cacactcata tcatcccgcg
    18001 aactcaggct acgttcatag cgcaacccag cctgagtttg cttgatggtt tttcgcgtgt
    18061 cgtattgttc agcgcgtggc gcttgttgcg gattcgcttt ccattctgct ttggttagcc
    18121 caccagggtc atctgctttg atatccacgc tattgaaaat caggcttaat ttgctggcgt
    18181 catcaatgcg tacgcccagt ttggcattag ctaaattttt ctgtgcgcca ctatggtcac
    18241 gatagccgtg ggtcgtaaaa cgcgtggttg agacggtgta atcgacatcg ccgggctgtg
    18301 tgccgtcgcc cgttgcaccc gttgctttga gcccatagcg ccagctgcca aaactgccgt
    18361 agtaactgct ggcttcaatg gttggtggct gttgcccggt ctgggtggtg acattcatta
    18421 caccgccaga cgcgttgcca tacagggcag agaaggggcc acgcagcact tccacatttt
    18481 gcacactgct taaatcgatg ttggatgttt gcccttgccc gtcgggcatg gtagcgggaa
    18541 taccgtccac atacaggcga ataccgcgaa ttccgtaagt ggagcgggag ccaaatccgc
    18601 gaatcgacag ctgtaaatct tgcgcatagt tctgccgatt ttgtacctgc aaaccaggca
    18661 cgctggtcag tgattcggac aagttaatgc gcggtgttgc caggcgcatc tcctcgccat
    18721 ccaccacgct tactgctgct ggggtatcca gttctgaaac cacctgcggt gcggcactga
    18781 caatcatggt ctgttcatca gcggcaaaaa caacggggga aagggcaagt agtgcgggca
    18841 aaacggtctg tcggacggaa aaaatcttca tgaaaaaagc caggttaaga atgggaaaac
    18901 gccgttatgg taatgaaatt gtaaatttat ggaaaatgaa acggcacgat acgttaagta
    18961 attgagaaaa ttgtagtcgt aacggcaaga aatgctccac atttgagaaa ataatgatta
    19021 ccattcccat ttataacaag agcgtaacga tgattacgct tagcgaagca ttgtgaagca
    19081 gcaaaaatat cggttcatca aagggagtcg tcatgcattt acgtcatctg ttttcatcgc
    19141 gcctgcgtgg ttcattactg ttaggttcat tgcttgttgc ttcatcattc agtacgcagg
    19201 ccgcagaaga aatgctgcgt aaagcggtag gtaaaggtgc ctacgaaatg gcttatagcc
    19261 agcaagaaaa cgcactgtgg ctcgccactt cgcaaagccg caaactggat aaaggtggcg
    19321 tggtttatcg tcttgatccg gtcactctgg aagtgacgca ggcgatccat aacgatctca
    19381 agccgtttgg tgccaccatc aataacacga ctcagacgtt gtggtttggt aacaccgtaa
    19441 acagcgcggt cacggcgata gatgccaaaa cgggcgaagt gaaaggccgt ctggtgctgg
    19501 atgatcgtaa gcgcacggaa gaggtgcgcc cgcttcaacc gcgtgagctg gtagctgatg
    19561 atgccacgaa caccgtttac atcagtggta ttggtaaaga gagcgtgatt tgggtcgttg
    19621 atggcgagaa tatcaaactg aaaaccgcca tccagaacac cggtaaaatg agtaccggtc
    19681 tggcgctgga tagcaaaggc aaacgtcttt acaccactaa cgctgacggc gaattgatta
    19741 ccatcgacac cgccgacaat aaaatcctca gccgtaaaaa gctgctggat gacggcaaag
    19801 agcacttctt tatcaacatt agccttgata ccgccaggca gcgtgcattt atcaccgatt
    19861 ctaaagccgc agaagtgtta gtggtcgata cccgtaatgg caatattctg gcgaaggttg
    19921 cggcaccaga atcactggct gtgctgttta acccagcgcg taatgaagcc tacgtaacgc
    19981 atcgtcaggc aggtaaagtc agtgtgattg acgcgaaaag ctataaagtg gtgaaaacgt
    20041 tcgatacgcc gactcatcca aacagcctgg cgctgtctgc cgatggcaaa acgctgtatg
    20101 tcagtgtgaa acaaaaatcc actaaacagc aggaagctac ccagccggac gatgtgattc
    20161 gtattgcgct gtaatagatg tggcgagggt gaaaacggag agacttgcct gacttttatc
    20221 acgccgcatg cggcacacct tcgtcagtaa tctaaatttg cccggcacgt agccgggcaa
    20281 ttttgcaatt agctcgccgt ttcaggctta aacacaattt cctgtttttc ttcatcttcc
    20341 tcgacgactg gcgcagtgct gtgaatttca gcaacgcgtt tgcgcacacc aaaccagcct
    20401 ataaccagca gaataccgat aatcggcagc gccgcgatag tataagtccc gttcgggtaa
    20461 tcgaacgcca tcagcaccag gacactcagt aaaaacagta atgtcagcca ggaagtgaag
    20521 ggcgcgccag gcagtttaaa actgacatcc gctgctttgc cttgtttaat tgctttacgc
    20581 aggcgcatct ggcacacgat gataaacgcc catgaagcga tgattcccag cgacgcgaag
    20641 ttcaacacaa tctcaaatac gcgcgacggc accagatagt tgaggaatac gccgacgaca
    20701 tacacaacta gtgtcgccag aatcccggca tacggcacat gctgacgact cattttcgcc
    20761 ataaaactcg gtgcggaacc gcccatcgcc attgagcgca gaatacgtcc ggtgcagtac
    20821 agacctgagt tcaggctgga gagggcagcg gtcagcacca caatgttcat aatgctgccg
    20881 atatatggca cacccagttt agagaaaaac gtcacgaacg gactttgccc cgcctgatac
    20941 gcgctccacg gcaatagcat aaccagcaac accacggagc cgacgtaaaa caggccaata
    21001 cgccaaatca cgctgttaat cgctttaggc accatggtct gcggatcttt acattctccg
    21061 gcagctgtac ccaccatttc aatggaggca aaagcaaaca ctacgccctg aatcaacacc
    21121 agagcaggca gcaaaccgtg ggggaagaag ccgccattat cggtaattaa atgaaagcca
    21181 gtggtgttgc catccagcgg ctgaccacta ctgaggaaca ctgtacccac gaccaaaaag
    21241 gtcacaatgg cgagcacttt aataagcgca aaccagaact ccatctccgc aaaccatttc
    21301 acaccgatca tattcatggt gccaacgatg gtaagtgcag cgagcgcaaa gacccactgc
    21361 ggcacgccgc caaacgcacc ccagtaatgc atatacagag cgacggcggt aatatcaaca
    21421 atccccgtca tcgcccagtt gatgaaatac atccagccag caacataagc ggctttctca
    21481 cccaaaaact cacgggcata agagacaaaa ctgccactgg aagggcggtg taacaccagc
    21541 tcacccaatg cacgtagaat aaaaaatgaa aacaggccgc agatcaaata gaccagtgcc
    21601 agtgcgggcc ccgccatttg cagtcgggct cctgcaccta aaaacaagcc ggtgccaatc
    21661 gcgccgccaa tggcgatcat ctgtacctgg cgattgccca tcgctttgtg atacccctct
    21721 tcgtgggcat taagccagcg gcgtttcgcg gcgtgttgat ctgaagtgtc ggtgtcgtgt
    21781 ttactcattg ctctccctga ttgctttaat gaaaaagtca tataagttgc catgaacaat
    21841 gtttattcat cctggcgatg atagttatca ccattattct ttatgggtat aaaactatcg
    21901 gcgcagcatc ctacccgtta tcgataaacg atgcaaaaca tccccttaca atcctgaagg
    21961 ggaataatac aactgacgaa aaaatgacaa atctttttgc tggttaacct gtgtactgtc
    22021 ctacacttaa tctttaaaag attgtgaggg catatgatta aggtgtatgg cgtacccgga
    22081 tggggctcta caatcagtga gctgatgcta acactggctg atattcctta tcagttcgtt
    22141 gatgtcagtg gttttgacca cgagggagct tcacgcgagt tattgaaaac cctgaacccg
    22201 ttgtgtcagg ttccgacact ggcgctggaa aatgacgaga ttatgacgga gacagcggcg
    22261 attgcattta tggttctcga tcgtcgcccg gaccttgcgc caccggttgg gcgcgccgaa
    22321 cgtcagcagt ttcaacggct attggtctgg ctggttgcta atgtctatcc aacattcact
    22381 ttcgccgatt accctgagcg ttgggcatct gacgcacctg aacagttaaa gaagaatgta
    22441 attgaaatct ggctgaattc acaattgacc gctgaacctt atgcctttgg tgagcaacta
    22501 acgctggtgg actgttatct ctgcaccatg cgcacatggg ggccggggca tgaatggttt
    22561 caggataatg cgcagaatat cagcgcgatc gctgatgctg tatgtcagtt accgaaatta
    22621 caagaagtat taaaaaggaa tgaaattat
//
LOCUS       DAPHBJ010000061        22605 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.061, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000061 DAPHBJ010000000
VERSION     DAPHBJ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.061"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..196
                     /locus_tag="RHZ76_002087"
     CDS             <1..196
                     /locus_tag="RHZ76_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="activator of nucleoside metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HDV2812729.1"
                     /translation="HEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLTKSKKGYLIKNRK
                     QLSGLALEMDPENKFSGMMQ"
     gene            complement(267..1517)
                     /locus_tag="RHZ76_002088"
     CDS             complement(267..1517)
                     /locus_tag="RHZ76_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDV2812730.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(1611..2549)
                     /gene="psuG"
                     /locus_tag="RHZ76_002089"
     CDS             complement(1611..2549)
                     /gene="psuG"
                     /locus_tag="RHZ76_002089"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HDV2812731.1"
                     /translation="MSKLKISPELLQISPEVQEALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(2537..3478)
                     /locus_tag="RHZ76_002090"
     CDS             complement(2537..3478)
                     /locus_tag="RHZ76_002090"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HDV2812732.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDIRGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(3902..5593)
                     /gene="fruA"
                     /locus_tag="RHZ76_002091"
     CDS             complement(3902..5593)
                     /gene="fruA"
                     /locus_tag="RHZ76_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HDV2812733.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(5610..6548)
                     /gene="fruK"
                     /locus_tag="RHZ76_002092"
     CDS             complement(5610..6548)
                     /gene="fruK"
                     /locus_tag="RHZ76_002092"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDV2812734.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(6548..7678)
                     /gene="fruB"
                     /locus_tag="RHZ76_002093"
     CDS             complement(6548..7678)
                     /gene="fruB"
                     /locus_tag="RHZ76_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HDV2812735.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVHAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPLAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            8046..9227
                     /gene="setB"
                     /locus_tag="RHZ76_002094"
     CDS             8046..9227
                     /gene="setB"
                     /locus_tag="RHZ76_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HDV2812736.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTIEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(9224..9478)
                     /locus_tag="RHZ76_002095"
     CDS             complement(9224..9478)
                     /locus_tag="RHZ76_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDV2812737.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEILTAP"
     gene            9633..10205
                     /gene="yeiP"
                     /locus_tag="RHZ76_002096"
     CDS             9633..10205
                     /gene="yeiP"
                     /locus_tag="RHZ76_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HDV2812738.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            10428..11894
                     /locus_tag="RHZ76_002097"
     CDS             10428..11894
                     /locus_tag="RHZ76_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDV2812739.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLVEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHQGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            12012..12998
                     /gene="yeiR"
                     /locus_tag="RHZ76_002098"
     CDS             12012..12998
                     /gene="yeiR"
                     /locus_tag="RHZ76_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HDV2812740.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            13037..13750
                     /gene="lpxT"
                     /locus_tag="RHZ76_002099"
     CDS             13037..13750
                     /gene="lpxT"
                     /locus_tag="RHZ76_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HDV2812741.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLGFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            14162..14728
                     /gene="mepS"
                     /locus_tag="RHZ76_002100"
     CDS             14162..14728
                     /gene="mepS"
                     /locus_tag="RHZ76_002100"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HDV2812742.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            14909..16465
                     /locus_tag="RHZ76_002101"
     CDS             14909..16465
                     /locus_tag="RHZ76_002101"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDV2812743.1"
                     /translation="MFIRAPNFGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARNAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            16547..18361
                     /locus_tag="RHZ76_002102"
     CDS             16547..18361
                     /locus_tag="RHZ76_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV2812744.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPSEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPTSSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPIYSLGIDTWWYDVNKAAKLPSASKQGE"
     gene            18362..19456
                     /locus_tag="RHZ76_002103"
     CDS             18362..19456
                     /locus_tag="RHZ76_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HDV2812745.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            19456..20481
                     /locus_tag="RHZ76_002104"
     CDS             19456..20481
                     /locus_tag="RHZ76_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2812746.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            20483..22072
                     /gene="yejF"
                     /locus_tag="RHZ76_002105"
     CDS             20483..22072
                     /gene="yejF"
                     /locus_tag="RHZ76_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HDV2812747.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIILRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(22076..22420)
                     /locus_tag="RHZ76_002106"
     CDS             complement(22076..22420)
                     /locus_tag="RHZ76_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812748.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
BASE COUNT         5461 a         5879 c         5533 g         5732 t
ORIGIN      
        1 tcacgaaaag catacgcaag cggcagagta tttaggcgtt tcttatcgac atcttttata
       61 tgttctcgcg caattcattc acgacggttt attaactaaa agcaagaaag ggtatctcat
      121 taaaaacaga aagcagttgt caggactggc gctggagatg gacccggaga ataaattctc
      181 cgggatgatg cagtaaaaat tatttgcaat agcgcgattg ccggatgcaa cgcttaatac
      241 gttttatccg gtctacaaat cgagcattac gccagaccaa taaagaaccc ggcaatagtc
      301 gcactcatca ggttggaaag cgttgctgct gccagcgccc gtaaaccaag ctgggcgatt
      361 tctggagcac gttttggcga aatagccgaa aatgcgccaa cgacaacacc gatagaacca
      421 aagttagcaa aaccacaaag cgcaaaggag ataatcgcaa tggttttcac ttccagcgtg
      481 ccgcccgttt gcaggtatgg ggagaaactc aggtaagcga cgaattcgtt aatcgccagt
      541 ttctgcccaa tcaggctacc cgcaagattg gcatcactcc agtccacacc catgatccac
      601 gccagcggtg ccagcacata gccaaaaata ctttccagag aggcattggc gaaaccaaac
      661 cagccaccaa ttccgccgat aatgccgttg atcagcgcaa taattgcgac aaacgccatt
      721 accaccgtcg ctacaccagc ggcgattttc agcccagtca tcgcaccgct cgccgccgct
      781 tcgataaagc tttttggcgg cgtttcgctg aacgacagat tttcaaatgt gacctgcgaa
      841 ggctcggtgg ccgggctaag aatacgtgca aacaaaatac cacctgggat cgccatcagc
      901 gatgccgcta acaggtagtc aattggtacg cccattccgg cataaccaat catcatcgaa
      961 ccagcaatgg acgccatccc gctacaaatt gcggtaaaca actcgttgcg attcatgcga
     1021 tcgataaacg gtttaacgat cgccgggatc tcattttgcc cgaggaaaat agttgtgact
     1081 gcgacaaaag attcgatttt gctgatgtta agagctttct ggaaaatgct gccaaggatg
     1141 cgaatcagca gccccatcac gccaatgtag tacagcagac tgatgagcgc agtaacgaaa
     1201 ataatcgccg gaagtacgcg aaaggcgaag ataaaacccg caccgtcaaa caggacatcc
     1261 attttcggcc caaccagcga accaaaaatg aaggcgctac cggcatcact gtaagacatc
     1321 actttatgaa cgcctaatgc cgcctgttct actgcccatt ttcccggtgg gaagtagagc
     1381 atgatgccac caatagcgat ttgcagcagc agtgcggctc caaccgtacg caaactgatg
     1441 ctctttttat tcactgacaa cagaaatgct attgccagta acaccaccat ccccacaaca
     1501 cttctcatta tgtccataat gattttccct tcatgccagt aaacccggcg tcagcgccag
     1561 gttttggtat gtttgatgag tacgggcgac ggctttctgc ccgtcagaaa ttaacccgcg
     1621 agacgctggt attctttggc aatttcactc gccagaatgg cgttgttgaa caccagctgg
     1681 atattggatt tcaggctgtc accgccggtc agttcagcaa cgcgcgccag caggaacggt
     1741 gtactttctt taccaataac gccctgctct tcggcttcgg cgacggcttg atctatggct
     1801 gcattgatag attcctccgg catcgcaaac tgttccggga tcgggttcgc taccacgagg
     1861 ccaccgttca gcccgctttg ccatttcacc gccattgcac gggcaatttc actggcgctg
     1921 tcgagacgaa tgctgacgtc aaacgagctg gtacggcaga aaaacgcagg cagcgcttta
     1981 gtctgatagc caattaacgg cacaccgaag gtttctaaat actcagtggt taatcctaaa
     2041 tcgagaatag atttcgcccc ggcacaaaca acggtgacat tagtatttgc cagttcttgc
     2101 aaatcggcag aaatatcgaa ggtatgttcc gccccgcgat gcacaccacc aattcccccg
     2161 gtggcaaata ctttaattcc ggcaagcgcc gcaataatca tcgttgaagc cacagtggtt
     2221 gcgccatttt ttccggcggc aacaacaaaa ggtaaatcgc gacgactaac tttggtcacg
     2281 ttatgccctt cacgacccag taattcaatt tcttctttac ttaaacccac tttcatcacg
     2341 ccgccaataa tggcgatagt ggcaggtact gcgccctgtt tacgaatagt ttcttctact
     2401 tcaattgcgg tctgggcatt ttgtgggaac ggcatcccgt gagaaataat ggtcgattcc
     2461 agcgccacaa ccggtttttt gttttttaaa gcttcctgca cttccgggga aatttgtaat
     2521 aattcagggg aaatttttaa tttagacatt ctgcgttctc cactaacgat ataacgttgg
     2581 caatcgataa atcggggtta ttggtgtatt cacaggagag cgccattgac gaacatccct
     2641 gtgcgaaacg aacagattcg gcaaacggca ttccgtctac ccaacacgaa gcaagtcccg
     2701 ccatcatggc atcgcccgct ccggtaacat taataacatt ggttttgatc ggcgcagacc
     2761 agccattttc acctctgata tcgctgtaat aaacgccgtc gccgcccatg ctcaatacca
     2821 gtcggttcag gccatgttga tggaaccagg cagcaacttt tgccacatcg tcacgccctg
     2881 acagcgcaat cccactcagg gtttccgctt caaggcggtt tggcttcaga gtgtggatct
     2941 gatttagacg gtcgcgcact ttgacacatt tccatgcgga aaccggatcg acaaatacgg
     3001 gtacgttggc ggcattatcc agaatccatg ccagtgcctc ttcactgata ttacagtccg
     3061 caacaatgac ctttgccctc tgaataaatt cacggtgctg tgcgagatat tcagctgtaa
     3121 tagcgttgct aatattcatg tcatttatag caaccagcat ttcaccggta ttatcgagta
     3181 atgataaata actagacgta ttttctcccg gcacaatcag gcatttatcg acataaacgc
     3241 cagattgatt ggtttgcgtt agcagcgatt gaccataaaa atcactgcct actgcgctca
     3301 gtagccaggc tttgttaccc agcaacgcca ggttttgtgc aatattgcgc cctactccac
     3361 caggcgtaaa ttttatttta cctggatttg aatccgcata atttaatgat tcatgtgaat
     3421 atccggcgac atcaatattc gccgaaccta taattacgac ataatccttt tcgcgcatag
     3481 cacgtcctct ggtaaaaaat taccaaccct gtcaatccac aagaatatag atactgcaaa
     3541 gacggtttaa acatgtgttc atatttaaac atgtgctcat agtatgcatg ttttacgtaa
     3601 agtaaattac cgccagagag gctaaatggg cagatttgag caggctcaca aaatgcgatt
     3661 aaatgcctga attacaaaag cgaggaattt gtgacgggat gcacagaaca ggaacaataa
     3721 aaaagggcag aaaataatct gcccttgagg aaataacctt agtgtttgat gaacgtttca
     3781 agtttctact gaaagataaa accatttaaa ttcataaaat tacattcaca agctggtcat
     3841 tccctttgcg ctcatccaat gatacagggc aggaaatttc tgccctgtaa cacacctttt
     3901 attacgctgc tttcgctact gcgtccactt ccggacgttt caggaaggca taggccaaac
     3961 ccgccaccag cgtaccggca ataattgcta ccaggtaacc cagtaccggc gtaatagcgc
     4021 cagggatcag cagaacaaac agaccaccgt gtggtgccat cagtttcgca ccaatcgcca
     4081 ttgagattgc gccagtcagc gcaccaccca cgatacagca cggcagcaca cgcatcggat
     4141 cacgagcagc aaacggaatt gcaccttccg aaatgaagca cagtcccaat accagagcgg
     4201 ctttgccacc ttcctgctgc gctttgtcga atttgcgacg cgccaccatt gttgccagac
     4261 ccattgccag cggtggcacc atacctgccg ccataatcgc cgccatcggg ccataggttt
     4321 gagtactcag cagacccaca ccgaatgcgt acgctgcttt gtttaccgga ccgcccatgt
     4381 cagtacacat catgccaccg aggatcgccc ccagcagaac cgcattcgca gtccccatgg
     4441 tctgcagcca gtgagtcagc ccttcgagaa tgccagcaac cggtttaccg atcaggtaga
     4501 tcatcgccag accgaccacc agactggaaa ttagcggaat gatcaggatt ggtttcagcg
     4561 cctccatact ctgtggcagt ttcagttgcg tactgattaa cttcgcaatg taaccagcca
     4621 ggaagcccgc aataataccg ccaatgaagc cagaaccggt gctgaccgcc agcataccgc
     4681 caatcagacc cggcgtgagg cccggacgat cggcaatgga aaaggcaata taacctgcca
     4741 gtaccggcac catcagcgca aaggctgaac caccaccaat ctgcatcagc gccgctgcca
     4801 acgtacccgg ctctttaaac gcttcgatac caaaagcaaa agaaagcgcg atacacagac
     4861 caccagcaac gaccatcggc agcatgtaag agacgcccgt tagcaagtga cgataagcgc
     4921 ctgcactctc tttcttacct tcagtggtcg ccgtttgagc tttgcccgcc ggttcatacg
     4981 gcgttgcttc agcaaccgct ttatccagtt cctgcgcggt tttcttcagc gccagaccgg
     5041 tagaggtacg atacatcggt ttaccagcaa atttcgccag atccacttcg atatctgccg
     5101 ccacaatcac cagatccgct gcggctactt cctcgggagt gattgcatta cccgcgccaa
     5161 cagaaccacg ggtttcaact ttcacccacc agccacgttt tttcgcttcg gtttcaatgg
     5221 cttcagccgc cataaaggtg tgtgctacgc cagtcgggca agcagtcacc gcaactacgc
     5281 gtttcggacc gctggcggca accggtgctg tcgcagtgac cggcgcagtg taaggtttcg
     5341 catggccttt ggcttcactc aggaacagct cagggtgcgc aactgcccgg gaaatatcac
     5401 ccagccagac atttttaccg ttcagcgcgc tgtcattcgg gatggaatca ccgagaacaa
     5461 tcgccatttc agcgtcgttc ggattgtcga tgatttccag ttttgctttt cgcgccgccg
     5521 cgcccagcag ggtcttcgcc atataggcgc gtgcctgacc gagattagcg tcaataatca
     5581 gcagcgtttt cattatgcct ctcctgctgt cagttaaaag gttgtaagtc gacgcgcgcc
     5641 atcattgcgg ccaactgcgg acgatcggta atacccacat tgctttgact taccgccagg
     5701 gctgcaacag ctgtcgccag acgcagtgtg tgttcactgg attcacgcat cagcaagcca
     5761 taaatcaggc caccaaccat agaatcccct gcgccaacgg tgcttacgac atcgactgac
     5821 ggtggtttgg cgatccattc gccggaggca ttaacccaaa gcgcgccttc ggcacccagt
     5881 gaaataacaa catgcgcgat accttgttca cgcagcgcat gcgcagcttc aatcacatct
     5941 ttcatttcag gcagtttacg gcctgcccag atttccagct cgcggcggtt aggtttcacc
     6001 agccacggtg ccgctttcaa acctgctact aacgcttcac ggctactatc aaagataatg
     6061 caaggacact gactacgcag gcgagtcatc cagtcggtga acgcttccgg gctgacgcct
     6121 gacggtaagc ttccgctgac acagaccata tcgaactgac cgagccagct cagagaatca
     6181 gtcacaaagc gttcccagtc ggcgggggtg acttcaaaac ccgagaagtt gaagtcggtc
     6241 acttcgccgt ctttttccgt cagcttaacg ttaattcgag tgcgcccctg tacaacctgg
     6301 aaacggttgg caatgcccag ctcgctgaac agttgctgaa aaccatcctg attgtcttta
     6361 ccgaggaagc cgccaacggt gacatcaatt cccaggtctt ttaatacttt ggccacgttg
     6421 atgcctttac ccgccgcatg cagaccggtg gttttcacca ggttcacttc gccgcgttca
     6481 atttccgggc agaaaccaac aaggtcataa gccggattaa gggtgatagt agcaacacgt
     6541 ctgctcatta tgcgccctcc ccaagaccag cagcgatagc gtcgccgatt gctttcagcg
     6601 cctgttcagc atctgcaccc tgggcggtaa agcgtaggcg atgacctttc ttaacgccaa
     6661 gtgccacaac tttcatcaga ctacgtccgt ttgccggttt gccggtgcca tcaaggtttg
     6721 tcacggtaat atcactgtta aattgtttaa tggtattgac cagcatggta cctggacgag
     6781 catgcaggcc gtgttcattg cgcaccacaa actccgcgct taacacgtcg tcggtcggcg
     6841 catcatcgct ggtcagcagc gccagcaacg ttgccgcatc cgctttcagc aagcggtcag
     6901 ctttattgtc gagcaacaaa tcagcgagac gcttaagaac cgcgaggggc tgatcgtcat
     6961 tcatcgccac actcaccagc atggctgccg tttcgccgtc cacatcaaaa gcatttgccg
     7021 cacggcttac cgcaatcgcg ctacgcagat tgccttcggc gctatcgctc agccagatac
     7081 cctgtccgag gttcagcggt tgttcattga tggctttggt gacgaaagtg gcgtcaactg
     7141 cccccgcctc tttcagacgc gcagcgttca gcgcctgaag agtcagcaga tcgctggcga
     7201 cgatatccag cgtcagcatt tcgttgtcga gcttcagctg ctcactctgc ttttcgccca
     7261 tcagcaatgc gcgaagttct tctgctgttg ttgctgactt cagttgttca gcaacggaat
     7321 catcgctcag tacgtgggtc agctggcgta gcaggcccag atgttcatcc gagctggcag
     7381 caataccgat cgccacgtac gctacctgac cgtcacccca ggtgacgcct tccgggaact
     7441 gaaatacctg aacgcccgtt ttcagcacct gatcgcgggt gtcggtggtg ccgtgtggaa
     7501 tagcaatacc attgccgagg aacgttgagg tttgctgctc gcgcgccagc atgccattga
     7561 cgtagccttc tgctacatta ccggcatgca ccagcgccgc agcgacctgg cgaatcgcct
     7621 cttctttgtc tccggccttt tcgcccggat ggatgtcctg tacggataac tggaacatag
     7681 ttctcctctc ttgctgaatt gaaacgattc agcctctatg agaaaaaaag cgccaacctg
     7741 gcttagggtt aaagacaaga tcgcgctgaa acgtttcaag aaagcataat acttctgttt
     7801 cagcacgcaa ggaaagccgt aaagttatga gcaaaagttt gatgtgctgc acaatttttt
     7861 gccatttttc ctaattgctg atgggaaaat ccgttatcag caatttcatt tcagcagcgt
     7921 cagcagccaa tcagcaacag tgaaatggca ttttgattat tcgtggcgct tttgatttcg
     7981 ctgtctgttt attttctgac aagcagcgta aactccgcgt cttcctcttc cagtgatcga
     8041 ccagcatgca taactccccc gcagtctcca gcgcgaaatc gtttgacctg acctcgacgg
     8101 cgtttttaat cgttgccttt ctcaccggta ttgcgggcgc tctgcaaacc ccgacactca
     8161 gtatttttct taccgatgaa gtacatgccc gtccggcgat ggtgggattc ttctttaccg
     8221 gcagcgctgt cattgggatt ctggtaagtc agtttctcgc cgggcgctct gataagcgcg
     8281 gcgatcgcaa atcgctgatt gtcttttgct gcctgttagg cgtgctggcc tgcacccttt
     8341 ttgcctggaa tcgcaactac tttgttttgc tattcgttgg cgtctttctt agcagctttg
     8401 gctcgaccgc taacccgcaa atgtttgccc ttgcccgtga acatgccgac aaaaccggac
     8461 gtgaggcggt gatgttcagt tcttttttac gcgctcaggt ttcactggca tgggtcattg
     8521 gcccaccgct ggcttatgcc ttagcgatgg gtttcagctt tacggtaatg tatctgagcg
     8581 cagcggtagc gtttattgtt tgcggcgtga tggtgtggct gtttttaccg tcgatgcaaa
     8641 aagagcttcc gctggcgacc ggcacgattg aagcgccgcg ccgtaaccgt cgcgatacgc
     8701 tgctgctgtt tgtcatttgt acattgatgt ggggctcgaa cagcctgtac atcatcaaca
     8761 tgccgctatt tattatcaac gaactgcatc ttcccgagaa actggccggt gtgatgatgg
     8821 ggaccgccgc cgggctggaa atcccgacca tgttgattgc cggatatttc gccaaacgtc
     8881 tgggtaagcg tttcttaatg cgcgttgctg ccgtgggtgg cgtctgtttt tacgcaggaa
     8941 tgctgatggc gcattcacct gtcattctgt tgggcttgca gctgctaaat gctattttta
     9001 ttggcattct gggtggcatc gggatgctct attttcagga tttgatgccc ggtcaggcag
     9061 gttcagccac cacgctctat accaacacgt cgcgcgtggg ctggatcatc gcaggatcag
     9121 tggcgggcat cgtcgccgag atctggaatt atcacgctgt gttctggttt gcgatggtga
     9181 tgattatcgc cactctgttt tgcttactgc ggattaaaga tgtttaaggc gcggtcagga
     9241 tttccagatc gataagccag gtcatggcct gctgacgcga gttaccgcac atttcagcac
     9301 tggcttgtaa gcctgcacag actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt
     9361 gctgttcatc aagctgaatg cagggcgtat tggcgggctt gccatctggc ataccgggaa
     9421 tagggctgga aattgaaggg gcagtgcaac acgccccgca acccggacgg cattccatac
     9481 gttgttctct taaggttaac caccggattg atgagcgcgc acagtaccac cttttttgca
     9541 ccagcaaaag tgcgaatacc acttgcccga aaggcccgtc gcgagtactt tgtcgcgata
     9601 tttttgacat tttcgactac aggaattttt cgatgccaag agcgaacgaa attaaaaaag
     9661 gtatggtact gaattacaac ggcaaactgc tgctggttaa ggatattgat attcagtcgc
     9721 ccactgcccg cggtgccgct acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc
     9781 tgaaagtaga agagcgtttc aaaggtgatg atatcgttga caccgtgacg ctgacccgcc
     9841 gttacgttga cttctcctat gtcgatggca acgaatatgt ctttatggat aaagaagact
     9901 ataccccgta taccttcacc aaagatcaga ttgaagaaga gttgctgttt atgccagagg
     9961 gcggcatgcc ggacatgcag gtgctgacct gggacggtca actgctggcg cttgagctgc
    10021 cgcagaccgt tgatctggaa atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca
    10081 gcgcccgtaa caaaccggcg acattgagca ctggtctggt gattcaggta ccagaatact
    10141 taagcccggg cgaaaaaatt cgtatccata tcgaagaacg ccgttatatg gggcgtgctg
    10201 actaacttca gccgcatgca gaaaagggat agctcaggct gtcccttttt taatttatta
    10261 taccaatctt ctattttgcg cttcctgaac accttacgcc ctcaattttc actcgttgat
    10321 tgatcgccct cacacttcat cgcattaaca atccagacca atttcaattg ctgtcatata
    10381 actttacact gtcattgtta atttatcgtt actaagacgt gactcctatg aagacaattg
    10441 cctccgttac gctcccgcat catgtacacg ctccacgcta cgatcgccag cagttgcaat
    10501 cacgtatcgt tcattttggc tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg
    10561 atcgtgtgct gaatgcccag ggcggcgact gggggatctg tgaaatcagc ttgttcagcg
    10621 gtgatcaact gatgagccag ctccgcgcac agaaccattt atataccgtg ctggagaaag
    10681 gtgcggacgg caatcaggtg ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac
    10741 tggattcctt agcggcaatt attgagaaat tttgcgagcc acaggtggca attgtttccc
    10801 tgacgattac cgaaaaaggc tattgtattg acccggccac cggtgcactc gacaccagta
    10861 atccgcggat tattcacgat ctacaaaccc ctgaagaacc tcactccgca ccgggtattc
    10921 tcgtcgaagc actgaaacgc cgccgtgagc gcggccttac accgtttacc gtgctctcct
    10981 gcgacaatat tcccgacaat ggtcatgtgg tgaaaaacgc ggtgctggga atggcagaaa
    11041 aacgttcgcc agaactcgcc gggtggataa aagagcacgt cagttttccg ggaaccatgg
    11101 tcgaccgcat tgttccggct gcaaccgacg aatcactggt ggaaatcagc cagcatctgg
    11161 gggtgaatga tccctgcgcg attagctgcg aaccgtttat ccagtgggtg gtggaagata
    11221 acttcgtcgc tgggcgtcct gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc
    11281 tgccatggga agagatgaaa ctgcggatgc ttaatggcag ccactctttt ctcgcttatc
    11341 tgggttacct ctcaggattc gcccatatca gtgattgcat gcaggatcgc gcatttcgcc
    11401 atgccgccag aacattaatg ctggatgagc aagcgccgac actgcaaatt aaagatgtcg
    11461 atttaacaca atatgcggat aagttaattg cacgttttgc taatccggcg ctgaaacata
    11521 agacctggca aatcgcgatg gatggcagcc agaaattacc gcaacgcatg ctggcaggta
    11581 ttcgcataca tcaggggcgc gaaacggact ggtcgttgct ggcattaggc gttgcaggct
    11641 ggatgcgtta cgtcagcggc gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc
    11701 ttagcgataa aattcgcgaa cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc
    11761 tgctttccct gcgtgaagtt ttcggtgatg atctgccaga taacccgcat tttgtgcagg
    11821 ccatcgaaca agcctggcaa caaatcgtac aattcggcgc acatcaggcg ctattaaaca
    11881 ccctcaaaat ttaacgattt ctgcggttaa agcggatgaa gctcaccttc gtccgctctc
    11941 cccttctctt ttctgccttt tttagccagg attaacgctc agttaactta ccagagtatt
    12001 gcggagccgt tgtgaccagg accaacctca tcaccggttt tctcggcagc gggaaaacca
    12061 cgtcgattct tcatctgtta gcccataaag atcccaacga aaaatgggcg gtactggtta
    12121 atgaatttgg ggaagtcgga attgatggtg ctttgctcgc cgatagcggc gcattgctga
    12181 aagagatccc cggcggctgc atgtgctgcg ttaatggttt acccatgcag gtagggttga
    12241 ataccttact gcgtcaggga aaaccagacc gcttgttgat agagccgacc gggctgggcc
    12301 atccgaaaca gatcctcgat ctgttaaccg caccagtcta tgaaccgtgg atagatctgc
    12361 gcgccacctt gtgcattctc gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg
    12421 aaaacttccg tgaccagctg gctgccgcag acatcattgt cgccaataaa tccgaccgtg
    12481 cgacgcccga aagtgagcaa gcgctacagc gttggtggca gcaaaatggt ggcgatcgac
    12541 aattaattca cagtgagcat gggaaagttg acggtcatct tctggattta ccgcgtcgca
    12601 atttagccga gttgcccgcc agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt
    12661 tagcagcgtt aagcctgcca gagcatcaac gctggcgtcg cagtctgaac agcgggcaag
    12721 gatatcaggc ctgcggctgg atattcgacg ctgatacggt attcgacacc attggcattc
    12781 tggaatgggc gcgacttgca ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag
    12841 ggctggtacg aatcaaccgt cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc
    12901 caccggacag ccgtattgag ctgatttcca gcagcgaagc tgactggaat gccttacaga
    12961 gcgcgctgtt gaagcttcgt ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa
    13021 gataattaga gaaaatatga ttaaaaattt gccgcaaata gtgttgttga atattgtcgg
    13081 cctcgcgctg tttctttcct ggtatattcc cgttaatcat ggattctggt tgccgattga
    13141 tgcggatatt ttttatttct ttaatcagaa actggtcgaa agtaaggcct ttttgtggct
    13201 ggttgcattg accaacaatc gcgctttcga cggttgttct ctgctggcga tgggaatgtt
    13261 gatgctgggt ttctggctga aagaaaacgc ccctggcaga cgacgtatcg tgattatggg
    13321 tctggtcatg ctattaactg cagtggtatt aaaccagctg ggtcaggcat taattcctgt
    13381 aaaacgggcc agcccaacat tgacttttac cgatattaac cgcgtcagcg aactgctctc
    13441 tgttcccacg aaagatgcct cacgagatag ctttcccggc gatcacggca tgatgctgct
    13501 tattttttcg gcattcatgt ggcgttattt cggcaaagtt gcaggcctta tcgcccttat
    13561 tatttttgtg gtttttgcat ttcccagagt aatgattggc gcacactggt ttactgacat
    13621 cattgtcggt tcgatgaccg tgatattgat cggtttgccc tgggtgttgc tgacgccatt
    13681 aagtgatcga ttaatcacct tttttgacaa atcactacct ggaaaaaaca aacatttcca
    13741 aaacaaataa ctcacagtaa ttaacatcat caggcttatt tttatagtga ggataatcct
    13801 gatgatgcgc accgtgcttt catccatcgc gcgcaaaaat cattctctaa gtaaatgaat
    13861 ggattgcatg cgtttcactc aatcgtactt taattgacca atcccgctta ttaactttct
    13921 gtatcacttt ttcttataaa aaatcatgca aaaccgctcg ccaagaccgc atcaatcggg
    13981 taatctcgaa ctcgttttgc ctcggcggta gattatcctc acagcatata attttgtgcg
    14041 ttagtccaca gatttggcct taaggaattg tttcaacatg cccaggtaat tagtctcgtg
    14101 tcgcttggca tttttttata acgatatttg tcgttaagga cttcaaggga aaacaaacaa
    14161 catggtcaaa tctcaaccga ttttgagata tatcttgcgc gggattcccg cgattgcagt
    14221 agcggttctg ctttctgcat gtagtgcaaa taacaccgca aagaatatgc atcctgagac
    14281 acgtgcagtg ggtagtgaaa catcatcact gcaagcttct caggatgaat ttgaaaacct
    14341 ggttcgtaat gtcgacgtaa aatcgcgaat tatggatcag tatgctgact ggaaaggcgt
    14401 acgttatcgt ctgggcggca gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg
    14461 tacattccgc gagcaatttg gcttagaact tccgcgttcg acttacgaac agcaggaaat
    14521 gggtaaatct gtttcccgca gtaatttacg tacgggtgat ttagttctgt tccgtgccgg
    14581 ttcaacggga cgccatgtcg gtatttatat cggcaacaat cagtttgtcc atgcttccac
    14641 cagcagtggt gttattattt ccagcatgaa tgaaccgtac tggaagaagc gttacaacga
    14701 agcacgccgg gttctcagcc gcagctaata aaccgtttgg atgcaatccc ttggctatcc
    14761 tgacgagtta actgaaagca ctgcttaggc agtgcttttt tgttttcatt catcagagaa
    14821 aatgatgttt ccgcgtcttg atccaggcta tagtccggtc attgttatct tttaaatgtt
    14881 gtcgtaattt caggaaatta acggaatcat gttcatacgc gctcccaatt ttggacgtaa
    14941 gctcctgctt acctgcattg ttgcaggcgt aatgattgcg atactggtga gttgccttca
    15001 gtttttagtg gcctggcata agcacgaagt caaatacgac acactgatta ccgacgtaca
    15061 aaagtatctc gatacctatt ttgccgacct gaaatccact actgaccggc tccagccgct
    15121 gaccttagat acctgccagc aagctaaccc cgaactgacc gcccgcgcag cgtttagcat
    15181 gaatgtccga acgtttgtgc tggtgaaaga taaaaaaaca ttctgttcat ctgcgaccgg
    15241 tgagatggac attccactca atgaattgat tccggcgctc gacattaata aaaacgtcga
    15301 tatggcgatc ttacccggca cgccgatggt gccgaacaaa cccgcaatcg tcatctggta
    15361 tcgcaaccct ttgctgaaaa atagcggcgt ctttgccgct ctgaatctca acctgacgcc
    15421 ttcactcttt tatagttcac ggcaggaaga ttacgatggc gtcgccctca ttattggcaa
    15481 tactgcgcta tctacctttt cttcacgttt gatgaacgtt aacgaattaa ccgacatgcc
    15541 agtccgtgaa actaaaattg cgggcattcc tctgaccgtt cggctttatg cagatgactg
    15601 gacatggaac gatgtgtggt acgcattttt actgggcggc atgagtggaa ctgtcgttgg
    15661 cctgctctgc tattacctga tgagcgtacg tatgcgcccc ggcagagaaa tcatgaccgc
    15721 catcaagcgc gaacaatttt acgtggcgta tcaaccggtg gtggatacac aagctttgcg
    15781 agtaacgggc ctggaagtac tgctacgctg gcggcatcct gtcgcgggag aaattccccc
    15841 ggatgccttc attaactttg ccgaatcgca aaagatgatt gtgccgctga ctcagcacct
    15901 gtttgagtta attgcccgca atgccgcaga attagaaaaa gtgctgccgg taggcgtcaa
    15961 atttggtatt aacattgcgc cggaccatct gcacagcgaa agctttaaag cagatatcca
    16021 gaaactgctc acttccctgc ccgcacacca tttccagatt gtgctggaaa ttaccgagcg
    16081 cgatatgttg aaagagcaag aagccacaca actcttcgcc tggctgcact cggtcggcgt
    16141 agaaattgct attgatgact tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt
    16201 tacgctcgat tatctgaaaa ttgaccgtgg atttatcaac gccatcggta cggaaacgat
    16261 cacttcaccc gtacttgacg cggtgctgac gctggcgaaa cgcctcaata tgctgacggt
    16321 tgctgaaggg gtcgaaacgc cggaacaggc gcgatggcta agcgaacgcg gcgttaattt
    16381 catgcaaggc tactggatta gtcgcccgtt accgctggac gattttgttc gctggctgaa
    16441 gaaaccgtat acgccgcagt ggtaaggtgt gcttacgtcc cttattattc atagtgaaag
    16501 catgccggat tgcggctaat gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat
    16561 actgctgctg tttatcgccc tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag
    16621 ctatgccttt gccgtgctgg gcgaaccccg gtacgcattt aatttcaacc attttgatta
    16681 tgtgaacccc gccgcgccaa aaggtgggca gataacgttg tcagccctcg gcaccttcga
    16741 taatttcaac cgctatgcac tgcgcggcaa cccgggcgca cgcaccgagc agttgtacga
    16801 cacgctattt acgacttccg atgacgaacc aggcagttat tacccgctga ttgctgaaag
    16861 tgcacgctat gctgacgatt attcctgggt ggaggtcgct attaatccgc gcgcccgttt
    16921 tcatgatggt tcgcccatta ctgcccgcga tgtagagttt acttttcaaa aatttatgac
    16981 cgaaggcgtg ccgcaatttc gtctggtcta caaaggcacc accgtcaaag ccattgcacc
    17041 gttaaccgtg cgcattgagt tagctaaacc cggcaaagaa gatatgctga gtctgttttc
    17101 gctgccggta tttccagaaa agtactggaa ggatcacaaa cttagcgacc cgctcgccac
    17161 gcctccgctt gccagtggtc cgtaccgcgt tacgtcctgg aaaatggggc aaaatattgt
    17221 ctattcccgt gtgaaagatt actgggcagc aaacttaccg gtaaaccgtg gacgctggaa
    17281 tttcgacacc attcgctacg attattacct cgatgataat gtcgcctttg aagcgtttaa
    17341 agcaggtgcc tttgatttgc gtatggaaaa cgacgccaaa aactgggcca cgcgttatac
    17401 cggtaaaaat ttcgataaaa aatacatcat caaagatgag caaaagaacg aatcagccca
    17461 ggatacgcgt tggctggcgt ttaatatcca acgtccggta ttcagcgatc gccgggtccg
    17521 ggaagctatc actctcgcct ttgactttga atggatgaac aaggcgttgt tttacaatgc
    17581 ctggagtcgc acgaacagtt attttcagaa taccgaatac gcggccagaa attaccccga
    17641 cgccgcggag ctggtgcttc tggcaccaat gaaaaaagat ctaccgtcag aagtcttcac
    17701 acaaatctac cagccgccgg tatccaaagg cgatggctac gatcgtgaca acctgttaaa
    17761 agccgacaaa cttctcaacg aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc
    17821 cacaacgggt cagccactca gctttgaatt attgcttccc acaagcagca atagtcagtg
    17881 ggtattgccg ttccagcaca gcctgcaacg gctgggtatc aacatggaca ttcgcaaggt
    17941 ggataactcg caaatcacta accgcatgcg cagtcgcgac tatgacatga tgccgcgcgt
    18001 atggcgggcg atgccgtggc ccagttccga tttacagatt tcctggtcat cggaatatat
    18061 caattccact tataatgccc ccggcgtgca aagcccggtt atcgactcgc tgatcaacca
    18121 aattattgcc gcgcagggaa ataaagaaaa attactgccg ttggggcgag cactggatcg
    18181 cgtattaacg tggaattatt acatgctgcc aatgtggtac atggcggaag accgtctcgc
    18241 ctggtgggat aaattctccc agccggccgt gcgccccatc tatagcctcg gtatcgatac
    18301 ctggtggtat gacgtcaata aagcggccaa actgccgtcc gccagcaaac agggagagta
    18361 gatgggcgct tacctgattc gccgtctgtt gctggtgatc ccaacattat gggcgattat
    18421 caccatcaac tttttcatcg tgcaaattgc gcctggcggt ccggtcgacc aggccatcgc
    18481 cgccattgag tttggtaatg ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc
    18541 cagccatgcg caaacgggtg tcggcaatat cagcgacagt aattaccgtg gcggacgcgg
    18601 attagatcca gaagtgatcg ctgagatcac tcatcgctac ggttttgata agccgatcca
    18661 cgaacgttac ttcaaaatgc tctgggacta catccgcttt gattttggcg atagcctgtt
    18721 tcgcagcgcc tcggtgctga cgctgattaa agacagtctg ccggtttcca tcaccctcgg
    18781 attgtggagc acgctgatta tctatctggt gtcgattccg ttaggcattc gcaaagctgt
    18841 ttataatggg agccgctttg acgtctggag tagcgcattt atcatcatcg gctacgccat
    18901 tccggccttt ttgtttgcca tcctgctgat tgtcttcttc gcgggcggca gctatttcga
    18961 cctgttccct ctacgcggcc tggtttccgc taactttgat tcgctgccgt ggtatcagaa
    19021 aatcaccgat tatctgtggc atatcacgct gccggtgctg gcgacagtga ttggtggctt
    19081 tgcggcgctg accatgctga caaaaaactc attccttgat gaagtgcgca agcaatacgt
    19141 ggtgaccgcg cgtgcgaaag gggtaagtga aaaaaatatt ctctggaaac atgtgttccg
    19201 caacgccatg ctgctggtga ttgccggttt tccggcgacg tttatcagca tgttttttac
    19261 cggctcgctg ctgattgagg tgatgttttc actcaatggt ctgggcttac tgggctacga
    19321 agcgaccgtc tcgcgcgatt atcctgtaat gtttggtacc ttgtatattt tcaccctgat
    19381 tggcctgctg ctgaatattg tcagtgatat cagctatacg ctggttgatc cgcgtattga
    19441 ttttgaggga cgttaatgtc gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt
    19501 cgtcataacc gtcgcggcta ctggtcgtta tggattttcc tcgtcttgtt tggtttgagt
    19561 ttgtgttctg aacttatcgc caacgataaa ccgttgctgg tgcgttatga cggcagttgg
    19621 tatttcccgt tattgaaaaa ctacagcgaa agcgattttg gcggcccgct ggcaagtcag
    19681 gctgattatc aggacccgtg gctgaaacaa cggctggaaa ataacggctg ggtactgtgg
    19741 gcaccgattc gctttggtgc taccagtatc aactttgcta ccgataagcc cttcccttct
    19801 ccaccctccc ggcaaaactg gctgggaacg gatgccaacg gcggcgatgt gctggcacgc
    19861 attctctatg gcacgcggat ctcggttctg tttggcctga tgctgactct ctgttccagc
    19921 gtgatgggcg tgctggcggg ggcgctacaa ggctattacg gcggtaaagt cgatctctgg
    19981 ggacaacgct ttattgaagt atggtcgggg atgccgacgc tgtttttgat tattttactt
    20041 tccagcgtcg tacagcctaa cttctggtgg ctgctggcaa ttactgtctt gtttggctgg
    20101 atgagtctgg tcggcgtggt gcgggcggag tttttacgta ctcgtaattt cgactacatt
    20161 cgtgcggcac aggcgcttgg cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct
    20221 aatgccatgg tcgcgaccct caccttttta ccgtttattt tatgtagttc gataaccacc
    20281 ctgacctcgc tcgatttcct cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa
    20341 ctgctgttac aagggaaaaa taaccttcag gccccgtggc ttgggatcac cgccttcttg
    20401 tcggtggcga tattattgtc tttgctgatc tttattggtg aagccgtccg cgacgcattt
    20461 gatcctaata aggcggtgta gcatgacgca aactctgtta gcgattgaaa atttgtcggt
    20521 gggttttcgc catcagcaaa ccgtacgtac agtagtcaat gatgtttcac tacagattga
    20581 ggctggcgaa acgctggcgc tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct
    20641 gtcaatttta cgcctgctcc cttccccgcc ggttgaatat ctctccggcg atattcgttt
    20701 tcatggcgaa tcgctgcttc acgccagcga tcaaacgttg cgcggtgtac gcggtaataa
    20761 gatcgccatg atttttcagg aaccgatggt gtcgttaaat ccattgcata ccctggaaaa
    20821 acagctttat gaagtgcttt cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga
    20881 aattcttaac tgccttgatc gcgttggtat ccgccaggcg gcaaaacggc tgacagatta
    20941 tccgcatcag ctctccggcg gcgaacggca gcgggtgatg attgcgatgg cgctgttaac
    21001 gcgaccggaa ttattaattg ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc
    21061 gcagatttta cagctgttgc gcgaactgca aggcgagctg aatatgggca tgctgtttat
    21121 tactcataac ctcagcattg tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg
    21181 tcgctgtgtc gagcaaaatt acgccgctac gctatttgca tcacccactc atccttacac
    21241 acaaaagcta ctcaacagtg aaccgtcagg cgatccagtg ccgttgccag aacctgcctc
    21301 aacgttgctg gatgttgaac agcttcaggt tgccttcccc attcgcaaag ggattttgaa
    21361 gcgcattgtg gatcataatg tggtggtgaa aaacatcagt tttacgctac gagcgggtga
    21421 aacactgggt ttagtgggcg agtccggttc cgggaaaagt acgacgggac tggcgctgct
    21481 gcgactgatt aattctcagg gcagcatcat ctttgacggt cagccactgc aaaatttaaa
    21541 tcgccgccag ctgttaccta ttcgtcatcg cattcaggtg gtatttcagg atccaaactc
    21601 ctcgctcaac ccacgactca acgttttgca gattattgag gaaggcttac gggttcacca
    21661 gccgacgctt tctgccgcac aacgcgaaca acaagtgata gccgtgatgc atgaagtggg
    21721 attagatcct gaaacacgcc accgttatcc ggcggagttc tctggtggtc agcgacaacg
    21781 tattgcgatt gccagggcat taattcttaa gccctcgctg atcatacttg atgaaccgac
    21841 atcatcactc gacaaaacgg tacaggcgca aatattgacg ctattgaaat cattgcaaca
    21901 aaagcatcaa ctggcctatt tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg
    21961 tcatcaggtt atcatactgc gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt
    22021 gtttgccaca ccgcagcagg agtatacgcg tcagctactg gcgttgagct gacgcttaaa
    22081 aaggattgta gtttgaaaag ggttcggcaa ttgccacacc aaaatttttc aatcggcatg
    22141 ttgcagcaaa ctcgtcctgg cgatttacaa acaggcacgg ctcaccttca cactccagca
    22201 ccgaacatgg aacttcgatg tcgcttaacg cctggcttag tttatacatc accgaccatg
    22261 cattatcacc atccgggcta agcaatttaa gcgccaccag gctgttgtca ccgcacggtc
    22321 cattttgcgg aatcggttca accttcgaac ctgcgaaacc agcagaccag cgatagttct
    22381 gcggcagtcg atggacgatt gagagttgta atgaagtcac ttattttccc cggaagcaca
    22441 ttcacttcac aatttgttta catcaatttt aacacatcat caacaatccg tctttataca
    22501 gatcgtaaag attgatgcct cgcctgcgtg gcctctatgg ctctgattta agtaatatct
    22561 gcgggcgtgg tcacgtttgc ggggctatat gtacccgttt ctgcg
//
LOCUS       DAPHBJ010000062        22485 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.062, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000062 DAPHBJ010000000
VERSION     DAPHBJ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.062"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            143..826
                     /locus_tag="RHZ76_002107"
     CDS             143..826
                     /locus_tag="RHZ76_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDV2812749.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIQEGKPESSLLKQEEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATESGSVIHISLVNLLSIPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMTSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPAA
                     DNRQKRSKK"
     gene            870..5894
                     /locus_tag="RHZ76_002108"
     CDS             870..5894
                     /locus_tag="RHZ76_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDV2812750.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     LLSSFGALADNYDGQGVDYGDGSASDGWVAIGKGAKANIFLNNAGASTALGYDAIAEG
                     QYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSFGQYSMALGRYSKALGRL
                     SIAMGDSSKADGANAIALGNAAKAAGIMSIGLGDNANASQDYAMALGAESEAAENATA
                     IGNKAHAKGVNSIALGNGSQALADSAIAIGQGNKANGADAIALGNGSQSSGLNAIALG
                     KASVVTGDNSLALGSNTNANGINSVALGAGSIADQDDSVSVGSDSLQRKIVNVKNGTI
                     KADSHDAINGSQLYAISDSVAKRLGGGSSVNVDDGTVKAPTYNLKNGNKNNVGDALTV
                     LDQFTLQWDQNRDKYSAAHGSSTASVITDVADGAVSDSSKDAVNGSQLKATNDDVETN
                     TTNIATNTGNIATNTANIATNTTNITNLTDTVGDLKDDALLWNGTAFNAAHGTETTST
                     ITNVKAGTLSDDSTDAVNGSQLKDTNDNVATNTTNIASNTANIATNTSNIADNTANIA
                     TNTSNIADNTANIATNTSNIAGNTANIATNTTNIAANTTSINSLNTSVDALEQDAMLW
                     NGTAFNAAHGTETTSTITNVKAGTLSDDSTDAVNGSQLKATNDNVATNTTNIASNTAN
                     IATNTANINTLNTSIDTLEQDAILWNGTAYSAAHGTETASTITNVKAGTLSENSTDAV
                     NGAQLNATNANVATNTTNIATNTASINTLNTSIDALEQDALLWDGTAFSAAHGANKDA
                     SKITNVLAGTVSSASTDAINGSQLHGLSSSIATYLGGGATVSDSGVFSGPTYNIDGND
                     YTNVGAALDAINTSLSDSLGDALLWDSTTGAFSAKHGSTASVITNVADGAVSDSSSDA
                     VNGSQLYDVSNSVVDVLGGGAGVNTDGSISAPTYTIANTDYDNVGDALNALDTTLDDA
                     MLWDATAGENGAFSASHDGSASKITNVAAGTISDTSTDAVNGAQLHGVSSSVAEALGG
                     GAAVNSDGSISAPTYTIADTDYTNVGDAMNAIDSTLDNALLWDATAGENGAFNASHDG
                     KASVITNVANGQINETSTDAVNGSQLNATNMLIQNIAGDTSESYITENGEGINYVRTN
                     DSGLVFEDASATGVGATAVGYNSVASGDSSVAIGQNSSSTIESGIALGSSSVSNRVIL
                     QGSRDTSVTEDGVVIGYNTSDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPSKYFHANSTEEDSLAVGEDSLAMGAKTIVNGDAGIGIGLNTLVLTDAI
                     NGIAIGSNASANHANSIAMGSGSQTTRGAQTDYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVADEENTISVGSS
                     TNQRRITNVAAGKNATDAVNVAQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6262..7917
                     /gene="lldP"
                     /locus_tag="RHZ76_002109"
     CDS             6262..7917
                     /gene="lldP"
                     /locus_tag="RHZ76_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDV2812751.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCIVGVITTLQAYVLTWMIP"
     gene            7917..8693
                     /gene="lldR"
                     /locus_tag="RHZ76_002110"
     CDS             7917..8693
                     /gene="lldR"
                     /locus_tag="RHZ76_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HDV2812752.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8690..9880
                     /gene="lldD"
                     /locus_tag="RHZ76_002111"
     CDS             8690..9880
                     /gene="lldD"
                     /locus_tag="RHZ76_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HDV2812753.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
     gene            10034..10507
                     /gene="trmL"
                     /locus_tag="RHZ76_002112"
     CDS             10034..10507
                     /gene="trmL"
                     /locus_tag="RHZ76_002112"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDV2812754.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10560..11381)
                     /gene="cysE"
                     /locus_tag="RHZ76_002113"
     CDS             complement(10560..11381)
                     /gene="cysE"
                     /locus_tag="RHZ76_002113"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDV2812755.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11461..12480)
                     /gene="gpsA"
                     /locus_tag="RHZ76_002114"
     CDS             complement(11461..12480)
                     /gene="gpsA"
                     /locus_tag="RHZ76_002114"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDV2812756.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAYRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12480..12947)
                     /gene="secB"
                     /locus_tag="RHZ76_002115"
     CDS             complement(12480..12947)
                     /gene="secB"
                     /locus_tag="RHZ76_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HDV2812757.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(13010..13261)
                     /gene="grxC"
                     /locus_tag="RHZ76_002116"
     CDS             complement(13010..13261)
                     /gene="grxC"
                     /locus_tag="RHZ76_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HDV2812758.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13403..13834)
                     /locus_tag="RHZ76_002117"
     CDS             complement(13403..13834)
                     /locus_tag="RHZ76_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDV2812759.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14079..15623
                     /gene="gpmM"
                     /locus_tag="RHZ76_002118"
                     /gene_synonym="pgmI"
     CDS             14079..15623
                     /gene="gpmM"
                     /locus_tag="RHZ76_002118"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDV2812760.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15633..16916
                     /gene="envC"
                     /locus_tag="RHZ76_002119"
     CDS             15633..16916
                     /gene="envC"
                     /locus_tag="RHZ76_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HDV2812761.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16920..17879
                     /locus_tag="RHZ76_002120"
     CDS             16920..17879
                     /locus_tag="RHZ76_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HDV2812762.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQIDTSTPPKNTVPDTPRNPFRGVKLCKPKKPLEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(17866..18900)
                     /gene="waaH"
                     /locus_tag="RHZ76_002121"
     CDS             complement(17866..18900)
                     /gene="waaH"
                     /locus_tag="RHZ76_002121"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HDV2812763.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEAATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCLRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRREVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLNRQGNKNLNYQRHYIKITRLLEKLNRNYTDKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(19139..20164)
                     /gene="tdh"
                     /locus_tag="RHZ76_002122"
     CDS             complement(19139..20164)
                     /gene="tdh"
                     /locus_tag="RHZ76_002122"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDV2812764.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(20174..21370)
                     /gene="kbl"
                     /locus_tag="RHZ76_002123"
     CDS             complement(20174..21370)
                     /gene="kbl"
                     /locus_tag="RHZ76_002123"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDV2812765.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(21646..>22485)
                     /gene="yibB"
                     /locus_tag="RHZ76_002124"
     CDS             complement(21646..>22485)
                     /gene="yibB"
                     /locus_tag="RHZ76_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HDV2812766.1"
                     /translation="IITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLAALENEM
                     IIFTSPDLKSRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRLEPRQLK
                     NPEYWAPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLKIWDFPF
                     DENKMHLFTIKKGLAVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLVLESQKI
                     TLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALRIFL
                     SRK"
BASE COUNT         5724 a         5810 c         5656 g         5295 t
ORIGIN      
        1 tacattttta tgatgaatgc ttaaaacaat atttaatcga cacacatcac tgtcattaat
       61 gtaaagcaac atagatcacc aataatacaa tggacaactc taccggcatt gatcgctggt
      121 cggaggcaat aaggattttc acatgaactt gaagaagatt tttttcagtg ctgtcactgt
      181 ttcagttctc tgcgcattaa cgggatgtga ctacattcaa gaaggcaagc cagaaagtag
      241 tttactgaaa caagaagaag aacataacaa taaaattgtg ctacttgaaa aacaacaagc
      301 acagctaaag agtcaactcg aaactattca aaaacaacaa actggcataa taaacagcac
      361 aaagacttta actcacgtaa ttaaatccgt taaagaccaa caaaatactt ttatttttac
      421 cgagtttaac ccagcaaaaa cgaagtattt catcctgaac aacggttcgg tggctttagc
      481 gggccgagtg ttatccattg acgccacaga gagtggcagt gttattcata tttcactggt
      541 caacttatta agtataccaa tctcaaacat tggctttaat gcgacatggg gtggtgaaaa
      601 acctgtcgat gccaaagagt ttgcccggtg gcaacaatta cttttcaaca catcaatgac
      661 atccacattg aaattattac caggtcaatg gcaagacatt aatttgacac tgaagggtgt
      721 atcgccaaat aacctgggat atctgaagtt agccatcaat atggaaaata ttcagtttga
      781 caatcttccc gctgctgata atcggcagaa aagaagcaaa aaataaatgc aattagtacg
      841 cttataacaa ggaatagcga gcatcattaa tgaacaaaat atttaaagtt atctggaacc
      901 ctgccacagg gaattatact gttaccagcg aaacggcaaa aagccgtggc aagaaatctg
      961 ggcgcagtaa gctgttaatt tctgcgctgg ttgcaggtgg gttgttgtca tcgtttgggg
     1021 cgctggctga taattacgac ggtcagggcg ttgattacgg cgatggctca gctagtgacg
     1081 gctgggttgc tatcggtaaa ggtgcaaaag caaatatttt tttaaacaac gctggtgcca
     1141 gtaccgcttt aggttatgac gctatagctg aaggccaata tagctctgcc atcggctcaa
     1201 aaacccatgc tattggtggt gcatcaatgg cctttggggt tagtgcaatc tctgaaggtg
     1261 acaggagtat cgcgctgggt gcatcgtcgt attcattcgg ccaatactca atggcccttg
     1321 gtcgttattc caaagcgctg ggtagattgt ctatagctat gggggatagc tccaaagcgg
     1381 atggagcaaa cgccattgcg ctgggaaatg ccgctaaggc tgctggtatt atgagcatcg
     1441 gtctcggtga taatgccaat gcgtcacaag attatgctat ggcgctggga gcagaaagcg
     1501 aagccgctga aaatgcgacc gctatcggca ataaagcgca tgcaaaagga gtgaatagca
     1561 tcgcgttggg taatggcagc caggctctgg cagatagtgc aattgctatt ggccagggca
     1621 acaaggctaa cggcgctgat gctatcgctc tgggtaatgg tagccagtcg agtggcttaa
     1681 acgctattgc cttaggtaag gccagtgttg taactggcga taatagtctt gcgcttggga
     1741 gcaataccaa tgccaacggt attaactctg tcgcgctggg cgcaggttct attgcggatc
     1801 aagacgatag cgtttctgtc ggcagtgact cattacaacg caagatcgtt aatgtaaaaa
     1861 atggcacgat caaggctgat agccacgacg ccattaatgg ctcacagctt tatgccatca
     1921 gcgactcggt ggcaaaacgg cttggcgggg gttcatcggt aaacgttgac gacggtactg
     1981 ttaaagcacc aacctacaat ttaaaaaatg gtaacaaaaa taacgttggc gatgcgctca
     2041 ctgtactcga tcaattcacc ctgcaatggg atcaaaacag agacaaatac agcgccgctc
     2101 atggtagctc aactgccagc gtaatcaccg atgtggcgga tggcgcagtt tcagattcca
     2161 gtaaagacgc ggttaacggt tcacaactga aagccaccaa tgatgatgtt gagaccaata
     2221 ccaccaatat cgctaccaat accggaaata ttgcaactaa cactgcaaat attgctacca
     2281 acaccaccaa tatcactaat ttgacagata ctgttggcga ccttaaagat gatgccctgc
     2341 tctggaatgg cactgcattc aacgccgctc atggtacgga aaccaccagc accatcacca
     2401 acgtgaaagc tggtaccctt tcggatgaca gtactgacgc ggttaatggc tcgcaactga
     2461 aagacactaa cgataacgtg gcgaccaaca ccaccaatat cgctagcaac acggctaaca
     2521 ttgccactaa caccagtaat attgccgata acactgccaa cattgctacc aacaccagta
     2581 atattgccga taacactgcc aacattgcga ccaacaccag caatattgca ggtaacactg
     2641 ccaacattgc taccaatacc actaatatcg cggctaacac cacaagcata aatagcctga
     2701 acacgtctgt ggatgctctt gaacaggatg ctatgctctg gaacggcact gcattcaacg
     2761 ccgctcatgg tacggaaacc accagcacca tcactaatgt gaaagctggc accctttcgg
     2821 atgacagtac tgacgcggtt aatggctcgc aactgaaagc tactaacgat aacgtggcaa
     2881 ccaacaccac caatatcgcc agcaatacgg ctaacattgc gaccaacacc gccaatatta
     2941 atactctcaa tacgtcgatc gatacccttg agcaggatgc aatactctgg aacggaacag
     3001 cgtatagcgc cgcgcacggt acagaaacag ccagcactat taccaacgtt aaagcaggta
     3061 ctttatctga aaatagtacg gacgcagtta acggtgcgca gctgaacgcg actaacgcga
     3121 atgtggcgac caacaccacc aatattgcta ctaacaccgc cagcattaat actctgaata
     3181 catccattga cgctctggaa caggatgcgc tgctttggga tggcactgca ttcagtgcag
     3241 cgcatggtgc aaacaaagat gccagcaaaa tcaccaatgt cctggcaggg actgtatcat
     3301 ccgccagtac tgatgccatt aatggcagcc agcttcatgg gttgagcagt tcgatcgcca
     3361 cctatcttgg tggtggtgcc actgtgagcg attctggcgt atttagcggc cctacctata
     3421 acattgatgg taatgattac accaatgttg gtgctgcact tgacgccatt aacacctcac
     3481 ttagcgactc actcggcgat gccctccttt gggatagcac gactggcgca tttagtgcca
     3541 aacacggttc taccgccagc gtaatcacta acgtcgcaga cggtgcagtc tctgactcta
     3601 gctctgatgc tgtgaatggt tcacaactgt acgatgtaag caactctgtt gtcgatgttc
     3661 tgggtggtgg tgctggcgtg aatacggatg gcagcatcag tgcaccaacg tacaccattg
     3721 ctaacactga ttacgataat gtcggcgatg ccctaaacgc gcttgatacc actcttgacg
     3781 atgcgatgct atgggatgct accgcaggtg aaaatggtgc cttcagtgcc agccacgatg
     3841 gcagtgccag caaaataact aacgtcgcgg caggaacaat ttctgacacc agcaccgatg
     3901 cggttaatgg ggctcaactc cacggcgtta gcagttccgt cgctgaggct cttggtggtg
     3961 gtgcggcggt gaattctgac ggcagcatca gcgcaccgac ttacaccatt gctgataccg
     4021 actataccaa cgtcggcgat gcgatgaatg caattgactc aaccctggat aacgccttgc
     4081 tttgggacgc taccgcaggt gaaaacggtg cgtttaacgc cagccatgat ggcaaagcca
     4141 gtgtcatcac caacgtagcc aatggtcaga ttaacgaaac cagcaccgac gcggttaacg
     4201 gttcccagtt aaatgccacg aatatgttga tccagaatat tgccggtgat accagcgaaa
     4261 gctatatcac agagaacggt gaaggtatca actatgtgcg tactaacgac agcggcttag
     4321 tctttgagga tgccagtgct acgggtgtgg gcgctacagc tgtaggttat aactccgtcg
     4381 catcaggcga tagcagtgtg gcaattggac agaacagcag cagtactatt gaatctggaa
     4441 ttgctctggg cagcagttct gtgtctaacc gtgtcatcct tcagggctcc cgtgatacca
     4501 gcgtaaccga agatggcgta gtcattggtt ataacaccag tgatggcgaa ctgcttggcg
     4561 cgttgtcaat tggtgatgac ggtaaatatc gtcaaatcat caacgtcgcc gatggttccg
     4621 aagcccatga cgccgttacg gttcgccagt tgcagaatgc gattggtgct gtagcaacga
     4681 caccgtccaa atacttccat gctaattcaa cggaagaaga ttcactggct gtcggtgaag
     4741 actcactggc aatgggtgcg aaaaccattg ttaatggtga tgccgggatc ggtattggcc
     4801 tgaacactct ggtgttaact gatgcaatca acggtattgc tatcggtagc aacgcgagtg
     4861 caaatcatgc aaacagtatt gcgatgggta gtggttccca gaccacccgt ggtgcgcaga
     4921 ctgactacac cgcctacaac atggacgcgc cgcagaattc tgtcggtgaa ttctctgtcg
     4981 gcagcgaaga cggtcaacgt cagatcacca acgtcgcggc tggttcagcg gataccgatg
     5041 cggttaacgt aggtcagttg aaagtcactg atgagcgcgt agcgcaaaat acccagagca
     5101 ttactaacct gaacaatcag gtcactaatc tggatactcg cgttactaat atcgaaaacg
     5161 gtattggcga cattgtcacc accggtagca ccaagtactt caagaccaac accgatggcg
     5221 tagatgccaa cgcccagggt aaagatagcg ttgctattgg ttctggttcc attgctgccg
     5281 ctgacaacag cgtcgcactg ggtaccggtt ccgttgcaga tgaagaaaat acaatctctg
     5341 taggttcttc cactaaccaa cgccgtatta ctaacgttgc cgcaggtaaa aatgctaccg
     5401 atgctgttaa cgttgcgcag ttgaagtctt ctgaggcggg cggcgtgcgt tacgacacca
     5461 aagctgatgg ttctatcgac tatagcaata tcaccctcgg tggcggcaac ggtggtacga
     5521 ctcgtatcag caacgtctcc gctggcgtca acaacaacga cgcggtgaac tacgcgcagt
     5581 tgaagcaaag cgtgcaggaa acgaagcaat acaccgatca gcggatggtt gagatggata
     5641 acaaactgtc taaaaccgaa agcaagttga gtggtggtat cgcttctgca atggcaatga
     5701 ccggtctgcc gcaggcttat acaccgggtg ccagcatggc ttctattggt ggcggtactt
     5761 acaacggtga atcggcagtt gctttaggtg tatcgatggt gagcgccaat ggtcgttggg
     5821 tctacaaatt acaaggtagt accaatagcc agggtgaata ctccgccgca ctcggtgccg
     5881 gtattcagtg gtaatgatcc attaagttag tgtgactaag ggcaaacgcc aaattgcctg
     5941 atgcgctacg cgcatcaggc aagataaggc gctctggatc aacaacctaa gggcaattct
     6001 ctgatgagga ttgccctttt ctttaccaga catctccccc cacaagaatt ggccctacca
     6061 attcttcgct tatctgacct ctggttcaca atttcccaat taaaactcac atcaatgttg
     6121 ccaatacata acatttagtt aaccattcat tgtcattatc cctacacaac acaattggca
     6181 gtgccacttt tacacaacgt gtgacaagga gatgagcaac agactcatta cacgatgtgc
     6241 gtggactcca ggagacctgc aatgaatctc tggcaacaaa actacgatcc cgccgggaat
     6301 atctggcttt ccagtctgat agcatcgctt cccatcctgt ttttcttctt tgcgctgatt
     6361 aagctcaaac tgaaaggata cgtcgccgcc tcgtggacgg tggcaatcgc ccttgccgtg
     6421 gctttgctgt tctataaaat gccggtcgct aacgcgctgg cctcggtggt ttatggtttc
     6481 ttctacgggt tgtggcccat cgcgtggatc attattgcag cggtgttcgt ctataagatc
     6541 tcggtgaaaa ccgggcagtt tgacatcatt cgctcgtcta ttctttcgat aacccctgac
     6601 cagcgtctgc aaatgctgat cgtcggtttc tgtttcggcg cgttccttga aggagccgca
     6661 ggctttggcg caccggtagc aattaccgcc gcattgctgg tcggcctggg ttttaaaccg
     6721 ctgtacgccg ccgggctgtg cctgattgtt aacaccgcgc cagtggcatt tggtgcgatg
     6781 ggcattccaa tcctggttgc cggacaggta acaggtatcg acagctttga gattggtcag
     6841 atggtggggc ggcagctacc gtttatgacc attatcgtgc tgttctggat catggcgatt
     6901 atggacggct ggcgcggtat caaagagacg tggcctgcgg tcgtggttgc gggcggctcg
     6961 tttgccatcg ctcagtacct tagctctaac ttcattgggc cggagctgcc ggacattatc
     7021 tcttcgctgg tatcactgct ctgcctgacg ctgttcctca aacgctggca gccagtgcgt
     7081 gtattccgtt ttggtgattt gggggcgtca caggttgata tgacgctggc ccacaccggt
     7141 tacactgcgg gtcaggtgtt acgtgcctgg acaccgttcc tgttcctgac agctaccgta
     7201 acactgtgga gtatcccgcc gtttaaagcc ctgttcgcat cgggtggcgc gctgtatgag
     7261 tgggtgatca atattccggt gccgtacctc gataaactgg ttgcccgtat gccgccagtg
     7321 gtcagcgagg ctacagccta tgccgccgtg tttaagtttg actggttctc tgccaccggc
     7381 accgccattc tgtttgctgc actgctctcg attgtctggc tgaagatgaa accgtctgac
     7441 gctatcagca ccttcggcag cacgctgaaa gaactggctc tgcccatcta ctccatcggt
     7501 atggtgctgg cattcgcctt tatttcgaac tattccggac tgtcatcaac actggcgctg
     7561 gcactggcgc acaccggtca tgcattcacc ttcttctcgc cgttcctcgg ctggctgggg
     7621 gtattcctga ccgggtcgga tacctcatct aacgccctgt tcgccgcgct gcaagccacc
     7681 gcagcacaac aaattggcgt ctctgatctg ttgctggttg ccgccaatac caccggtggc
     7741 gtcaccggta agatgatctc cccgcaatct atcgctatcg cctgtgcggc ggtaggcctg
     7801 gtgggcaaag agtctgattt gttccgcttt actgtcaaac acagcctgat cttcacctgt
     7861 atagtgggcg tgatcaccac gcttcaggct tatgtcttaa cgtggatgat tccttaatga
     7921 ttgttttacc cagacgcctg tcagacgagg ttgccgatcg tgtgcgggcg ctgattgatg
     7981 aaaaaaacct ggaagcgggc atgaagttgc ccgctgagcg ccaactggcg atgcaactcg
     8041 gcgtatcacg taattcactg cgcgaggcgc tggcaaaact ggtgagtgaa ggcgtgctgc
     8101 tcagtcgacg cggcggcggg acgtttattc gctggcgtca tgacacatgg tcggagcaaa
     8161 acatcgtcca gccgctaaaa acactgatgg ccgatgatcc ggattacagt ttcgatattc
     8221 tggaagcccg ctacgccatt gaagccagca ccgcatggca tgcggcaatg cgcgccacac
     8281 ctggcgacaa agaaaagatt cagctttgct ttgaagcaac gctaagtgaa gacccggata
     8341 tcgcctcaca agcggacgtt cgttttcatc tggcgattgc cgaagcctca cataacatcg
     8401 tgctgctgca aaccatgcgc ggtttcttcg atgtcctgca atcctcagtg aagcatagcc
     8461 gtcagcggat gtatctggtg ccaccggttt tttcacaact gaccgaacaa catcaggctg
     8521 tcattgacgc catttttgcc ggtgatgctg acggggcgcg taaagcaatg atggcgcacc
     8581 ttagttttgt tcacaccacc atgaaacgat tcgatgaaga tcaggctcgc cacgcacgga
     8641 ttacccgcct gcccggtgag cataatgagc attcgaggga gaaaaacgca tgattatttc
     8701 cgcagccagc gattatcgcg ccgcagcgca acgcattctg ccgccgttcc tgttccacta
     8761 tatggatggt ggtgcatatt ctgaatacac gctgcgccgc aacgtggaag atttgtcaga
     8821 agtggcgctg cgccagcgta ttctgaaaaa catgtccgac ttaagcctgg aaacgacgct
     8881 gtttaatgag aaattgtcga tgccggtggc actggctccg gtgggtttgt gtggcatgta
     8941 tgcgcgtcgt ggcgaagttc aggcagccaa agcggcggac gcgcatggta ttccgtttac
     9001 tctctcgacg gtttccgttt gcccgattga agaagtcgcg ccagccatca agcgcccaat
     9061 gtggttccag ctttatgtac tgcgcgatcg cggctttatg cgtaacgcgc tggaacgagc
     9121 aaaagcagcg ggttgttcga cgctggtttt caccgtggat atgccgacac cgggcgcacg
     9181 ctaccgtgat gcgcattcag gtatgagcgg cccgaacgcg gcaatgcgcc gctacttgca
     9241 agcggtgaca catccgcaat gggcgtggga tgtgggcctg aacggtcgtc cacatgattt
     9301 aggtaatatc tcagcttatc tcggcaaacc gaccggactg gaagattaca tcggctggct
     9361 ggggaataac ttcgatccgt ccatctcatg gaaagacctt gaatggatcc gcgatttctg
     9421 ggatggcccg atggtgatca aagggatcct cgatccggaa gatgcgcgcg atgcagtacg
     9481 ttttggtgct gatggaattg tggtttctaa ccacggtggc cgccagctgg acggtgtact
     9541 ctcttccgcc cgtgcactgc ctgctattgc agatgcggtg aaaggtgata tagccattct
     9601 ggcggatagc ggaattcgta acgggcttga tgtcgtgcgt atgattgcgc tcggtgccga
     9661 caccgtactg ctgggtcgtg ctttcttgta tgcgctggca acagcgggcc aggcgggtgt
     9721 agctaacctg ctaaatctga tcgaaaaaga gatgaaagtg gcgatgacgc tgactggcgc
     9781 gaaatcgatc agcgaaatta cgcaagattc gctggtgcag gggctgggta aagagttgcc
     9841 tgcggcactg gccccgatgg cgaaagggaa tgcggcatag tcgtttgcgc cccctccccc
     9901 taaccctctc cctcagggag ctgtctcttg atcagatctc ctgatcaaga gacaggggga
     9961 ggccggggtg aggggaaaag gccgcactgc tcaccaattt tctgctatcc tgcccccgca
    10021 ctaagggggc agtatgctaa acatcgtact ttacgaacca gaaattccgc caaatactgg
    10081 caacatcatc cgtctttgcg ctaataccgg ctttcgtctg catatcatcg aaccgatggg
    10141 atttgcctgg gacgataagc gcctgcgccg cgcggggctg gactatcacg agtttaccgc
    10201 cgttacgcgt catcatgact ttcgcgcgtt cctcgaagca gaaaatcccc agcgcctgtt
    10261 tgccctcacc acgaaaggta cgcctgctca cagcgccgta agctatcagg atggcgatta
    10321 tctgatgttc ggcccggaaa cacgcggcct gccagcgagc attcttgatg ccctgcccgc
    10381 tgaacaaaaa attcgcattc cgatggtgcc ggacagccgc agcatgaacc tgtccaatgc
    10441 ggtgtcagtt gtggtgtatg aagcctggcg gcagctggga tatccgggag cgttgttgag
    10501 agattagtta ctgtatgccg gatagatgat tacatcgcat ccggcacgat cacaggacat
    10561 tagatcccat ccccatactc aaatgtatgg ttaataccgt tgaaatgctg gtccatatcc
    10621 attgatggct tatcgctgtc tggtttaccg acaatacgag ccggaacgcc agcggcggtt
    10681 gtatgcggcg gcaccggttg cagcaccacg gaacctgcgc caatcttcgc gccgcgacca
    10741 acttcaatat tgccgaggat tttcgcgccc gcgccaatca tcacaccttc acgaattttc
    10801 gggtgacggt caccaccaga tttacccgta ccgccaagcg tcacagattg cagaatcgat
    10861 acgtcgtttt caattaccgc cgtttcaccc acaacgatgc ccgtggcgtg gtcgagcata
    10921 atgccacggc caatttttgc tgccgggtga atatcgacct ggaacgtcac agaaacctgg
    10981 ttttgcagaa agattgccaa tgcgcgacgc ccctgattcc acaaccagtg accgatgcga
    11041 taggcctgca aggcatgaaa acccttcagg tataacaacg gggttgagta tttatcgact
    11101 gccgggtcgc gggtacgcac cgcctgaata tcacaggccg cagaggcgat catttccggg
    11161 tcagcggcgt aggcttcttc caccacttca cggatagcaa tagcaggcat aattggcgat
    11221 gacagcttgt tcgccagcat gtagctcagt gcacttccaa ggttttcgtg cttgagtagc
    11281 gtcgcgtggt aaaaactggc cagcattggc tcacagtccg ccagcgttct ggcttcggct
    11341 ttaatattgt tccagacaat ttccagttct tcacacgaca ttgcttactc cacacgatga
    11401 gataatgacc ggcccgttct gcgcgggccg ggtcatagcg gtaacaaagg ttccctgggg
    11461 ttagtggctg ctgcgctcgt ccttgcgtgc acgacctaat aacgtcaatg ctgcctcgcg
    11521 cgcgtttttt ccgcaatata atacttgata aatttcctcg gttattggca tttctacgcc
    11581 gaagcgatac gccagttcgc ggacttcttt cgtattgcgg tagccttcca ccacctgacc
    11641 aatcttctcc tgcgcgcttt gtacatccat gccctgaccg agcatcatgc caaaacggcg
    11701 gttacgcgac tggttgtcgg tacaggtaag cacaagatcg cccagccccg ccatgcccat
    11761 aaaggtggca ggatcggcac ccagcgccgc gccaagacgc gacatttcag ccagcccacg
    11821 ggtgatcagt gccgtacgcg cattcgcacc aaaaccgata ccgtcggaca tccccgcgcc
    11881 aatggcaatg acgtttttca ccgcaccgcc aagctgcacg ccaatgaaat ccgggttgct
    11941 gtaaacgcgg aaacttttgc cacagtgcag caattgttgg agatcatcgg caaaggtctg
    12001 gtcggtagag gccagcgaaa tagctgtcgg taaacctgcc gccagttctt tcgcaaacgt
    12061 tgggccagag ataaccgcca gcggaatttg atcgcctaac gcctcgcggg ccacgtcctg
    12121 taacagacgc ccggtttccg cttccagccc tttggtcgcc cacaccagac gcgcatcagg
    12181 acgcatcagc ggtttaatct ggcgcagcac ttcaccaaag acatggctgg gtacgacgac
    12241 gagaatatta cggctggctg ccagcgcagt ggcgagatcg ctttcaagat ggagcgtatc
    12301 gggaaaaggc acatcgggga gaaacgcggc gttacagcgg tcgcgttcaa gcgttgcgat
    12361 atgttcaggg tcatggcccc agaggacaac ctcgtggcca tttcttgcca gggtaatggc
    12421 aagagcggtg ccgtacgagc cggcaccgat cacagtcatt gaagcattac gttggttcat
    12481 caggcatcct gatgttcttc agtaccttcg ccagcctgct gctgcaaata gttcatgaac
    12541 agcgcatcga agttaaccgg cgcaaggttc agttgcggga atgtaccgcg ggataccatg
    12601 ctggtgatgc actcacgagc atacgggaac agaatgttcg ggcagtatgc gcccaggcaa
    12661 tgtgccatct gggtgccttc gatacccgcg atggagaaaa taccgccctg ctgaacttca
    12721 cacaggaacg cggtttcttc gcccagggag gccgttacgg taacacgcag taccacttcg
    12781 taaacgtcat ctgccagttg agtagatgcc gtatccagat caagtttaac ttctggctgc
    12841 caatctttct ggaaaacgtg cggcgcgttc ggcgcttcga aagaaatatc cttggtataa
    12901 atacgttgga tctggaaagt catttcggtg ttgttttgtt ctgacatgtg tagaaaaccc
    12961 ttaagtgttg tccttaaata ctgcgcaatg taatgccaac gttcacacgt tatttcagca
    13021 ggggatccag tccaccacgt gcatccaatg catacaagtc atcacagccg ccaatgtgct
    13081 gtgcgtcaat aaaaatctgg ggaaccgtgg tgcgaccgct gcgtttgatc atctcttcac
    13141 gcttggcggc attgccatcg atcggcaact cctgaaaact cacgcccttg ctgctcagca
    13201 gtgcttttgc acgatggcaa tacgggcagg tttctttggt atagatttca acattggcca
    13261 taatttagct cctgtttttt taccctgaaa tttcatgttg cagggcggct gcaaaatgga
    13321 gtgtccccag aagcttataa caataagtcg ctgggggcct aattgcagct aacggcctgc
    13381 atcatgaaag acgacaggta aattatttgc cgcgcaccaa aggcaagttt tcgccagccc
    13441 agccagcgac gccttctttc agcacgaata cttgcgcaaa accagctttc gtcagtgcgt
    13501 ttgcaggctc ctggcactgc atgccagaac cgtctaccac gataaccggt ttgtctttgt
    13561 gcttctcaag ctcaccaaca ttgttggctt tgatttcgct cggcaacaga ttgatagaac
    13621 ctgcgatatg gcctttacgg aagtcatcac gctgacgtaa atccacaacc acagcgtctt
    13681 ctttgttgat cagacgcgta gcttcaccac gagtaatcac cttcactttc gaggtcaggc
    13741 tcttaaacgt agtcacaaga accgccacca gtaacgcgat ccaggcgata ctcagtatgg
    13801 gatgacggcc aacaaattgc ataatttctt gcatgggggg taacaactcc cgacgtagtg
    13861 attaaaaaaa ccaggaaagg agtatacctg cgcggtgcgg caaatacagc cagcgcgtta
    13921 actggaatgc aattttgcgg ggcacgacga aaaaaaagcg caaatctggc atcggcttgc
    13981 cgcgagcggt cgtatatttt gatcttcaga ggctatttta tcgattcagc tgtagtaaaa
    14041 ttacgcaaat tttgactctt gagtatgagg ttgtcgcaat gtcggtttct aaaaaaccta
    14101 tggtactggt gattctggat ggctatggct atcgtgaaga acagcaggat aacgccattt
    14161 ttagtgctaa aaccccggta atggatgcac tgtgggccaa tcgtccgcat accctgatcg
    14221 acgcttccgg tctggaagtc ggtctgcctg accgtcagat gggtaactcc gaagtaggcc
    14281 acgttaacct gggtgccggc cgcatcgtgt atcaggacct gactcgtctg gacgttgaaa
    14341 tcaaagatcg cgctttcttt gctaatccag tgctgactgg cgcggtagat aaagcgaaaa
    14401 acgcaggtaa agcagtacac attatgggtc tgctctccgc aggcggcgta cacagccacg
    14461 aagatcacat catggcgatg gtagaactgg cagctgaacg cggcgcagaa aaaatctacc
    14521 tgcacgcatt ccttgacggt cgcgacaccc cgccgcgcag tgctgaatcc tcgctgaaaa
    14581 aatttgaaga gaaatttgct gcgctgggta aaggccgcgt agcgtccatc attggtcgtt
    14641 actacgcgat ggaccgcgat aaccgttggg atcgcgttga aaaagcctat gacctgctga
    14701 ctctggcgca gggtgagttc caggccgata ctgccgttgc tggtttgcag gcagcttatg
    14761 ctcgcgacga aaacgacgag ttcgtgaaag cgaccgttat tcgtgcagaa ggtcagccag
    14821 atgcggcaat ggaagacggc gatgcgctga ttttcatgaa cttccgtgct gaccgcgcgc
    14881 gcgaaatcac tcgtgcattc gtaaacgcag atttcgacgg cttcgcgcgt aagaaagtgg
    14941 ttaacgtcga tttcgtgatg ctgaccgaat acgccgctga catcaaaact gcggttgctt
    15001 acccacccgc ttccctggtt aacaccttcg gcgagtggat ggcgaaaaac gacaaaactc
    15061 agttgcgtat ttccgaaacc gaaaaatatg cccacgttac tttcttcttc aacggtggcg
    15121 tagaagagtc gttcaaaggc gaagatcgca ttctgatcaa ctcaccgaaa gtggctacct
    15181 acgatctgca accggaaatg agctccgcag agctgaccga aaaactggtt gcggccatca
    15241 agagcggcaa atacgacacc attatctgta actatccgaa cggtgacatg gtaggtcaca
    15301 ccggggtgat ggaagcggcg gttaaagcgg ttgaagcact ggatcactgc gtggaagaag
    15361 tcgcgaaagc ggttgagtcc gtgggtggac aactgctgat caccgctgac cacggtaacg
    15421 ctgaacagat gcgcgatccg gcaacgggtc aggcacacac ggcacacacc aacctgccag
    15481 ttccgctgat ttacgttggt gataagaacg tgaaagcggt tgcaggcggc aaactttctg
    15541 acatcgcgcc gaccatgttg tcgctgatgg gtatggaaat cccgcaagag atgactggta
    15601 agccgctgtt catcgtggaa taatccctcc ccatgagggg aaaggcgatt aataccatga
    15661 cacgggccgt gaaaccgcgc aggtttgcaa tcaggcccat catctacgcc agcgttctta
    15721 gcgctggcgt attgttgtgc gccttttccg cccatgcgga tgagcgtgac caactcaaat
    15781 ctattcaggc cgatatcgcc gcgaaagagc gcgcggtacg ccaaaagcaa caacaacgcg
    15841 caagcctgct cgcacaattg aaaaagcagg aagaagcgat ctctgaagcc acccgtaagc
    15901 tgcgcgaaac gcaaaacacg ctcaatcaac tcaataaaca gattgatgag atgaacgcgt
    15961 cgattgccaa actggagcag caaaaagccg cccaggagcg cagcctcgcc gcacaactgg
    16021 atgccgcatt ccgtcagggc gagcataccg gtattcagct gattctcagc ggtgaagaaa
    16081 gccagcgtgg acagcgttta caggcttatt tcggctatct caaccaggcg cgacaagaaa
    16141 ccattgccca gttgaagcaa acgcgtgaag aagtcgccat gcagcgtgct gaactggaag
    16201 agaaacaaag cgagcaacaa acgctgttat atgaacagcg cgcccaacag gcgaagctga
    16261 cccaggcgct gaacgagcgt aaaaagacgc tggcagggct ggagtcttcc atccagcaag
    16321 gtcagcaaca gttgagcgag ctgcgcgcca acgaatcccg tctgcgtaac agcattgccc
    16381 gtgcggaagc cgcggcgaaa gcgcgtgctg aacgagaagc gcgtgaggcc caggcggttc
    16441 gcgaccgcca gaaagaagcg acgcgcaaag gcaccaccta caaaccgacc gaaagcgaaa
    16501 aatcgctgat gtcccgaact ggtggcctgg gtgcaccgcg tggtcaggca ttctggccag
    16561 ttcgcgggcc gacgctgcat cgctatggcg agcagctaca gggtgaatta cgctggaaag
    16621 gaatggttat cggtgcctct gaaggtactg aagttaaagc gattgccgac ggtcgggtga
    16681 ttctggctga ctggctgcaa ggctacggtc tggtggtggt ggttgagcat ggtaaaggcg
    16741 acatgagtct ttacggctat aatcagagcg cactggtgag cgttggttcg caggttcgcg
    16801 cgggccagcc gattgcactg gtgggcagca gtggcggtca gggtcggcct tcactctatt
    16861 tcgaaattcg ccgccagggt caggcggtca atccgcagcc gtggttggga agataagttt
    16921 tgtttccatt tcgtcgtaac gttcttgcat ttgccgctct gttggcgctc tcctcccccg
    16981 taattgctgg caagcttgcc atcgtcattg atgattttgg gtatcgcccg cacaacgaaa
    17041 accaggtgct ggcgatgcct tccgctatct ccgtcgctgt attacccgat tcaccgcacg
    17101 ccagagagat ggcgaccaaa gcgcataaca gcggtcacga agtgttgatt catctaccga
    17161 tggcaccact gagtaaacag ccgctggaga aaaatacgct acgcccggag atgagcagcg
    17221 acgaaattga gcgcattatt cgtagtgcgg tcaataacgt gccctatgcc gtggggatca
    17281 acaaccacat gggcagcaag atgacctcta acctgtttgg tatgcagaaa gtgatgcagg
    17341 cgctggagcg ttacaatctt tatttcctcg acagcgtaac catcggtaat acccaggcga
    17401 tgcgcgcagc gcaaggcact ggcgtgaagg tgatcaaacg gaaagtgttc ctcgacgatt
    17461 cgcaaaatga agcggacatc cgtgtgcaat ttaatcgcgc aattgatctg gcgcgtcgca
    17521 acggttcaac cattgccatt gggcatcctc acccttcaac ggtacgcgtg ctacaacaga
    17581 tggtttataa cctgccgcca gacattacgc tggtgaaagc cagcagcttg ctgaatgaac
    17641 cgcagattga tacgtctaca ccgccgaaaa acactgtgcc cgacacaccg cgtaatccgt
    17701 tccgtggcgt gaagctctgc aaaccgaaga aaccgctgga acctgtttat gctaatcgct
    17761 tctttgaagt attaagcgaa agcatcagcc agagcacgct gatcgtttac ttccagcatc
    17821 agtggcaagg ctggggcaaa cagcctgaag cggcgaagct taacgctagc gcaaattaaa
    17881 ggcgctacgc gtaatgcgat ggtgcgaccg cgttttgtcg cgccattgcc ataagcggaa
    17941 agaccacagt aacgcctgat aacccacctt cacgctgcgc acattggtaa tcaggcgctt
    18001 atacataccg gaagtaaata tctcggcaat catccgttgg cgggtaataa tatccggctc
    18061 tttacgcacc gcatggcaaa cacgcaatgc ttcgtaagtt atttgctgat gaaattccgg
    18121 ataaatcgta attttgtcgg tataatttcg atttaatttc tctagcaggc gggtaatctt
    18181 aatatagtga cgttgataat taaggttttt attcccttgt ctattcaacc gactcactga
    18241 cgtattatgc agataatatt tatataacga ttgctcggta tatcgcgcac gcagcgcgtt
    18301 aaacatgaat tctgttgtcc agacaatatc ctgatgatgt aatccggcaa taaatttaat
    18361 gttatttttt acaataacct cgcggcgata aacccccatc cagacaacgt gagtccaacg
    18421 gcgcgaagaa agccccatcc gcagccagtc cgggccggtt aatacgccgg ttgagcgaag
    18481 gcgatcggtg gggatggatt gccaggtttc tcccgtttca cgaagacacc agtcagcgtt
    18541 gcactgcgcc acgtcgaggt cgtcctctaa cgccatggtc atcagcgttt cgtacatggt
    18601 gggatagacc tcatcgtcag catcgacaaa agcgacatat ttacccgtcg ccgcctcgat
    18661 cccacgatta cgcgccaccg atgcgccagc attcgcctga tgcaacaaac gaacgtgcgg
    18721 atagttttct gcgtaatgct ttgctatttc aacagaatta tccgttgaac catcgttaat
    18781 aataatgatt tccagagcag tccaggtttg cgtaattaaa gattccatac aagtgcggaa
    18841 atcatcaccc gcattatata acgggataat aacactaagt ttattggtgc tgttcatcat
    18901 aaattaccga cgacctctga agttatcctg acgttttaca ggctttgtat taagaaacta
    18961 ttaagcctgt gagatattat ttttacaggt gagagagtca cttcagggta gagagataat
    19021 gagaacagct atataagaga tcagcgcaat tacccattct ttattttact gacagcattt
    19081 tacatattta acagattatc ctcaggaaaa agggctggaa taccagccct tgtttgtgtt
    19141 aatcccagct caggataact ttcccggact ggcccgaacg catggcgtca aagcccttct
    19201 ggaaatcatc gatagagaaa cgatgggtaa tgatcggcga aagatcgaga ccagactgaa
    19261 tcagcgccgc catcttgtac caggtttcaa acatctcacg accgtaaata cctttaatga
    19321 acaagccttt aaagatcact ttggtccagt cgatagacat atcagacggt ggaataccca
    19381 gcatcgcaat acgaccgccg tggttcatgg tgtcgagcat ggtacgaaac gctggcggcg
    19441 caccggacat ttccagaccg acatcaaagc cttcggtcat gcccagttct gccatcacat
    19501 cattgaggtt ctctttagcg acgttaaccg cacgggtgat acccatttta cgcgccaact
    19561 caaggcggta ttcgttaaca tcagtaatca ccacgttacg tgcaccaacg tgtttcgcca
    19621 ccgccgccgc cataatacca atcgggcctg caccagagac cagcacatcc tcgcccacca
    19681 gatcaaacga cagcgcggta tgcacggcgt taccgaaggg atcaaaaatg gaagccagat
    19741 catcggagat attgtcgggg attttgaaag cgttgaacgc cggaatcacc agatactcgg
    19801 cgaagcagcc cggacggtta acgccgacgc cgatcgtgtt gcggcacaga tgggtacgac
    19861 cgccacgaca gttgcgacag tgaccacagg tgatatggcc ttcgccagaa acgcgatcgc
    19921 caatcttgaa gcctttcact tcctgaccaa tacctaccac ttcaccgaca tattcatggc
    19981 ccacgaccat cggcaccggg atggtttttt gcgaccactc atcccagtta tagatgtgaa
    20041 cgtcagtccc gcagatggct gttttacgga ttttaatcag cagatcgtta tgcccgagtt
    20101 ccggtacagg aacgtcggtc atccagatgc cctcttccgc tttcagtttg gataacgctt
    20161 tcatctcaca tcctcaggcg ataacgccca gttgtttacc aatacgcgtg aatgcttcta
    20221 ctgcacgcgt aatttgctca ggggtatgcg ccgcagacat ctgggtacga atacgcgcct
    20281 gacctttcgg aacgaccgga tagaagaaac cggtgacgta aatgccctct ttttgcagct
    20341 cacgggcaaa tttctgcgcc actactgcat caccaagcat gaccggaata atggcgtgat
    20401 cggctcccgc cagggtaaag cccgccgccg acatttgctc acggaactga cgcgcgttcg
    20461 cccacagacg gtcacgcagc tcgctacccg cttcaaccat ctccagcact ttgatggaag
    20521 cggcaacaat ggccggtgcc agcgagttgg agaacaggta cggacgagaa cgctggcgca
    20581 gccactcaac cacttctttg cgcgccgcgg tataaccacc agaagccccg cccagcgctt
    20641 taccgagcgt gccggtgata atatcgaccc ggcccatcac atcgcagtat tcatgggaac
    20701 cacgaccgtt ttcaccgaca aaacctaccg cgtgggagtc gtctaccatc accagggcat
    20761 catatttatc tgccagatcg caaacgcctt tcaggttggc aatcacgcca tccatcgaga
    20821 acacaccatc ggtggcaatc agcacatgac gcgcaccggc ttcacgcgct tctttcagac
    20881 gtgcttccag ctcctgcata tcgttgttgg cataacgata gcgtttagct ttgcacagac
    20941 gcacaccatc aataatagac gcgtggttca gtgcgtcgga gataatggcg tcttccgcgc
    21001 ccaacagcgt ttcaaacagg ccaccgttag catcaaagca ggaagagtag agaatcgcat
    21061 cttccatccc caggaatgcc gccagttttt gttcaagctc tttatggctg tcctgagtgc
    21121 cgcagataaa acgcaccgaa gccatgccga aaccgtgaga atccattccc gcctttgccg
    21181 ccgcaatcag atccggatga ttcgccagtc cgagatagtt attggcacaa aagttaataa
    21241 cgtggcttcc atcagccaca gtgatatctg cttgctgcgc agacgtaata atgcgctctt
    21301 ctttaaacaa cccttccgcc cgtgcggttt ccagatcgtt ggttaactgc tgataaaatt
    21361 ctccacgcat tgcgattctc cagacagggc aaattccagc acatattacc caaacttata
    21421 ggttgcgacg agatgacgcg ttaacacttc tgcaaaattc aggataaata acgtgaatct
    21481 tgatgtctct caacacctat agatagtaga aaaactgtac agttttggct gggcataatg
    21541 atgagtgatt ttactgcatt gatataaaaa gaaaaaatga tgccaaagca tcattttttc
    21601 ctatctcagt tggcatcaaa acgctaccca ctaatggagg gaaacctatt ttcttgaaag
    21661 gaaaattctc agcgcctgca tttttgcccc taaagtgtta ctcctgaagc agcgaaacaa
    21721 atcgaaccat tttcctctcc ccagatagtt taggttgaag agatcggagc gtttgtaata
    21781 gcacatgaca aaaatgccct ggtcatcgtc cacaatgtta ttgtttaggg ttattttttg
    21841 actttccaga actaatttat agaattcttt ccatttatgc tgtgaaccaa caattgcgcc
    21901 accaataata tatacatgat tgccaatcat gaaatcaaaa gcctgttgtt gagatgtgac
    21961 agcaagccct ttttttatag tgaaaagatg catcttattt tcatcaaatg ggaaatccca
    22021 aattttcaat cctcgagtta catttggctt acggcaatag ccaaaatcta tccacgcaac
    22081 aagaggagtt ttcactaagc ccatgttgat agctttattt acaaaatagg ccttaaggtt
    22141 acataccaat acatactctg gtgcccagta ctctgggttt tttaattgcc gcggctcaag
    22201 tctatttgta aacgactcat ctttttgaat tttttcgata cgacttctga tatatctaaa
    22261 tttctttttt atatcgataa cgataaccgt tgttggtttt ccgttgcgaa tcgcttcaac
    22321 ccttgatttc aaatcgggag aagtgaaaat aatcatctca ttttcaagtg cggccagacg
    22381 ctcaaagtaa ctaaaataaa catcaactga acgagctagt ttttcacgga accctttatt
    22441 agcagtccaa tcccctctac caatgtcaaa atatgcagtg ataat
//
LOCUS       DAPHBJ010000063        22423 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.063, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000063 DAPHBJ010000000
VERSION     DAPHBJ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.063"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..130)
                     /locus_tag="RHZ76_002125"
     CDS             complement(<1..130)
                     /locus_tag="RHZ76_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDV2812767.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVIN"
     gene            complement(140..691)
                     /gene="ybcL"
                     /locus_tag="RHZ76_002126"
     CDS             complement(140..691)
                     /gene="ybcL"
                     /locus_tag="RHZ76_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HDV2812768.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(843..1193)
                     /gene="emrE"
                     /locus_tag="RHZ76_002127"
     CDS             complement(843..1193)
                     /gene="emrE"
                     /locus_tag="RHZ76_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HDV2812769.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPHIKNNHP"
     gene            complement(1527..1841)
                     /gene="fliE"
                     /locus_tag="RHZ76_002128"
     CDS             complement(1527..1841)
                     /gene="fliE"
                     /locus_tag="RHZ76_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDV2812770.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2056..3714
                     /gene="fliF"
                     /locus_tag="RHZ76_002129"
     CDS             2056..3714
                     /gene="fliF"
                     /locus_tag="RHZ76_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDV2812771.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            3707..4702
                     /gene="fliG"
                     /locus_tag="RHZ76_002130"
     CDS             3707..4702
                     /gene="fliG"
                     /locus_tag="RHZ76_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDV2812772.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4695..5381
                     /gene="fliH"
                     /locus_tag="RHZ76_002131"
     CDS             4695..5381
                     /gene="fliH"
                     /locus_tag="RHZ76_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDV2812773.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5381..6754
                     /gene="fliI"
                     /locus_tag="RHZ76_002132"
     CDS             5381..6754
                     /gene="fliI"
                     /locus_tag="RHZ76_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HDV2812774.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLRGLE
                     RIFPTVS"
     gene            6773..7216
                     /gene="fliJ"
                     /locus_tag="RHZ76_002133"
     CDS             6773..7216
                     /gene="fliJ"
                     /locus_tag="RHZ76_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HDV2812775.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7213..8340
                     /gene="fliK"
                     /locus_tag="RHZ76_002134"
     CDS             7213..8340
                     /gene="fliK"
                     /locus_tag="RHZ76_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HDV2812776.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQVRVTGNSGVDIFA"
     gene            8445..8909
                     /gene="fliL"
                     /locus_tag="RHZ76_002135"
     CDS             8445..8909
                     /gene="fliL"
                     /locus_tag="RHZ76_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HDV2812777.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8914..9918
                     /gene="fliM"
                     /locus_tag="RHZ76_002136"
     CDS             8914..9918
                     /gene="fliM"
                     /locus_tag="RHZ76_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HDV2812778.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9915..10328
                     /gene="fliN"
                     /locus_tag="RHZ76_002137"
     CDS             9915..10328
                     /gene="fliN"
                     /locus_tag="RHZ76_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HDV2812779.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10331..10696
                     /gene="fliO"
                     /locus_tag="RHZ76_002138"
     CDS             10331..10696
                     /gene="fliO"
                     /locus_tag="RHZ76_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HDV2812780.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQPDFQSVMKNLLKRSGRS"
     gene            10696..11433
                     /gene="fliP"
                     /locus_tag="RHZ76_002139"
     CDS             10696..11433
                     /gene="fliP"
                     /locus_tag="RHZ76_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDV2812781.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11443..11712
                     /gene="fliQ"
                     /locus_tag="RHZ76_002140"
     CDS             11443..11712
                     /gene="fliQ"
                     /locus_tag="RHZ76_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDV2812782.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11720..12505
                     /gene="fliR"
                     /locus_tag="RHZ76_002141"
     CDS             11720..12505
                     /gene="fliR"
                     /locus_tag="RHZ76_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HDV2812783.1"
                     /translation="MLQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            12795..13418
                     /gene="rcsA"
                     /locus_tag="RHZ76_002142"
     CDS             12795..13418
                     /gene="rcsA"
                     /locus_tag="RHZ76_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HDV2812784.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVANGIFVNMR"
     gene            complement(13462..13650)
                     /gene="dsrB"
                     /locus_tag="RHZ76_002143"
     CDS             complement(13462..13650)
                     /gene="dsrB"
                     /locus_tag="RHZ76_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HDV2812785.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            13813..14040
                     /gene="yodD"
                     /locus_tag="RHZ76_002144"
     CDS             13813..14040
                     /gene="yodD"
                     /locus_tag="RHZ76_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HDV2812786.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            14338..15153
                     /locus_tag="RHZ76_002145"
     CDS             14338..15153
                     /locus_tag="RHZ76_002145"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HDV2812787.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(15150..16844)
                     /gene="yedQ"
                     /locus_tag="RHZ76_002146"
     CDS             complement(15150..16844)
                     /gene="yedQ"
                     /locus_tag="RHZ76_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HDV2812788.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVLRAGFYVSTQPTLFTRNVPTRYYSYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISNSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(17082..17264)
                     /locus_tag="RHZ76_002147"
     CDS             complement(17082..17264)
                     /locus_tag="RHZ76_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HDV2812789.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRPAEEV"
     gene            complement(17343..18260)
                     /locus_tag="RHZ76_002148"
     CDS             complement(17343..18260)
                     /locus_tag="RHZ76_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HDV2812790.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            18433..19353
                     /gene="yedA"
                     /locus_tag="RHZ76_002149"
     CDS             18433..19353
                     /gene="yedA"
                     /locus_tag="RHZ76_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HDV2812791.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(19342..19812)
                     /gene="vsr"
                     /locus_tag="RHZ76_002150"
     CDS             complement(19342..19812)
                     /gene="vsr"
                     /locus_tag="RHZ76_002150"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HDV2812792.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(19793..21211)
                     /gene="dcm"
                     /locus_tag="RHZ76_002151"
     CDS             complement(19793..21211)
                     /gene="dcm"
                     /locus_tag="RHZ76_002151"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HDV2812793.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(21278..21973)
                     /locus_tag="RHZ76_002152"
     CDS             complement(21278..21973)
                     /locus_tag="RHZ76_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDV2812794.1"
                     /translation="MDLQHWQAEFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQHSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTMEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPLAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTVRGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(22013..22378)
                     /locus_tag="RHZ76_002153"
     CDS             complement(22013..22378)
                     /locus_tag="RHZ76_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDV2812795.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMACYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
BASE COUNT         5471 a         5930 c         5706 g         5316 t
ORIGIN      
        1 aattgataac tttttttccc tgacggatac gacataaagc tggcataaga ggcttaaccc
       61 tgtgaatctc atgatgttta tgcatccgta tttcttcaat ctttaggtta gtcctatctt
      121 ttgccagcat actcttatcc tactttacct cataaacggg tattatctca gcggttgcaa
      181 ttttattggc attaagcata taaccaacta acgctccgct ggaattagaa tccacaggaa
      241 tcttatcagt ttttagagcc catattttaa actggtaatg atggggttta tctcctttgg
      301 gaggacatgc tccaccaaat cccgcatagc caaaatcatt tcggccttgg acagcaccag
      361 tcggaagttt tgtaccatca cgtcttcctg catcagcggg caaatatgtt accgttgctg
      421 gaatattagc aacagtccag tgccaccatc cgctgcctgt aggcgcatct ggatcataca
      481 cagttacggc aaagcttttg gtgccttctg gagcaccaga ccaggttaat gagggcgatg
      541 tattaccccc ctcacaccca aatccagaaa atacatgaga cgttgtaagt tgctctcctg
      601 tttttatttc attactagtg acctgaaatg ctgcggcctg cgcagaaaat gttatgaatg
      661 ccaatacact tgaaacgata agttttttca taaaaacctc tttgttgtga cctatcgtta
      721 ttttatttga tgtgcattga tctcattatg cataaaggcg caatgatcgc gcaaatgcaa
      781 tcataattat aaagttgccc cgaacattag ccataatatg cacaaatcag attcctgtta
      841 tctcaaggat gattattttt tatatgcggt gcgcttcgtg acaacaagtt aatgactaac
      901 acaccggcac aaatcaacat catgcctatg atggctggca ggtccagtcg ttggccaaaa
      961 aatccccagg aaagcaaact aatcagaacg ataccgactc ctgaccagat agcataagca
     1021 atccctgtag gtatataagc cagtgtctga gctaataacc aaaatgatgc acaatagcaa
     1081 ataattgtgc caacagaggg ccataaccgt gtaaacccct ctgaaaactt cattaaggtt
     1141 gtaccaatga cctctgcaag tattgcgcca ccaagataaa tataagggtt catagcatat
     1201 tctttcctgt tcatactgga gagaattgta ctacagtttg aacacaactc accgttttca
     1261 tcctggtgta tgtgtatcca gggggctctg gcgtggcaag agataaaggc aggaacaata
     1321 atgatcagaa ggaaccccag gctaacaaaa gagttcactc ttcctatgag caaagcgaag
     1381 acaaaacatg ttatcgcgtt tacggatcgt ggatggcaga aaacgttcag gatcaggtat
     1441 ccattttaaa ccagaaattg agtgagtttg ccccatccat gccccaagcg gtgagattgg
     1501 aagtaattac caacaggtta caaaacctac acctgcatgc tcatcacttc ctgatacgcc
     1561 gccaccagct tattacgcac ctgaatcccc atttgcatag aaactgaggc tttttgcata
     1621 tcggtcatca catcgtttaa cgccacgccg ggttcaccga gagtgaattt ttctgcctgc
     1681 gtgcgggcgg ctgtttgtgt atcgcttatg cgatcgagcg cggcgtgcag ctgcccggca
     1741 aaactaatgg tcggttgtgg cagtgattcc tgtgcacgcg cactcatcgc cgtagcctgt
     1801 aactggctga taaccccttc aatcccctgt atcgctgaca ttttcatctc ccggataatt
     1861 tctgttagca aagcctacca gtaagtcaat aagacaaagg cgctaaatag caacaaaaaa
     1921 acgggtttat tggcggatag aaaaaaacga aagcacaaat aatgggagcg tcaatttttc
     1981 gagtttgctg acccgggagt gagtcttgtt ccactttgcc aataacgccg tccataatca
     2041 gccacgaggt gcgcgatgaa tgcgactgca gcccagacaa aatctcttga gtggcttaat
     2101 cgcctgcgtg cgaatccgaa aattccattg attgttgccg gttccgcggc agtggcggtc
     2161 atggtcgcac tgatcctgtg ggcgaaagcc cccgactacc gcacattatt cagcaatctt
     2221 tccgatcagg atggtggcgc aattgtcagc caactgacgc aaatgaatat tccttaccgc
     2281 ttcagcgaag ccagcggcgc tattgaagtt ccggcagata aagttcacga gctgcgcctg
     2341 cgcctggcgc aacaaggttt gcccaaaggc ggcgcggtcg gtttcgaact gctcgatcag
     2401 gaaaagtttg gtatcagcca gttcagcgaa caggtgaatt atcagcgggc gctggaaggc
     2461 gagctttctc gtaccatcga aactatcggc ccggtaaaag gggcgcgcgt acatctggca
     2521 atgccgaaac cgtctttatt cgtccgtgaa caaaaatccc cttctgcatc ggtgacggta
     2581 aatctgttac ccggccgcgc actcgatgaa gggcaaatta gcgccattgt gcatctggtt
     2641 tccagtgcgg ttgctggtct gccgccgggg aacgtcacgc tggtggatca gggcggacat
     2701 ctgttaaccc agtccaatac cagcgggcgc gatcttaatg acgctcagtt gaaatatgcc
     2761 agcgatgtcg aaggccgtat tcagcggcgt attgaagcga tcctgtcgcc gattgtcggc
     2821 aacggtaata ttcacgccca ggtcacggcg cagctggact tcgccagtaa agaacaaacg
     2881 gaagaacagt atcgccctaa cggtgatgaa tctcatgcgg cgcttcgttc acgccagctt
     2941 aatgagagcg agcaaagcgg ttccggttat ccgggcggcg taccgggggc gttgtcgaat
     3001 caaccggcac ctgcgaataa cgcgccaatc agcacgcctc cggcaaatca aaataaccgc
     3061 cagcagcagg cgagcaccac cagcaatagt gggccgcgta gcacacagcg gaatgaaacc
     3121 agtaactacg aagtcgatcg caccattcgt cataccaaaa tgaacgtggg cgatgtgcaa
     3181 cgtctgtcag tcgcggttgt ggtgaattac aaaaccttgc cagatggcaa accgttgcct
     3241 ctcagcaacg aacagatgaa gcaaattgaa gatctgaccc gcgaggcgat gggcttttct
     3301 gaaaaacgcg gtgactcgct caatgtcgtt aactcgccgt tcaatagcag tgacgaaagc
     3361 ggcggagaac tgccgttctg gcaacagcaa gcgtttatcg atcagttgct ggctgccggt
     3421 cgctggttgc tggtactgct ggtggcgtgg ctgctgtggc ggaaagcggt acgcccgcag
     3481 ttaacacgtc gcgccgaggc gatgaaagct gtacagcaac aggcgcaggc ccgtgaggaa
     3541 gtggaagatg cggtggaagt ccgcctgagc aaagacgaac aactccaaca acgacgcgct
     3601 aaccaacgtc tgggggcaga agtcatgagc cagcgtatcc gtgaaatgtc tgataacgat
     3661 ccgcgcgtgg tggcgctggt cattcgccag tggataaata acgatcatga gtaacctgac
     3721 aggcaccgat aaaagcgtca tcctgctgat gaccattggc gaagaccggg cggcagaggt
     3781 gttcaagcac ctctcccagc gcgaagtgca aaccctgagc gctgcaatgg cgaacgtcac
     3841 gcagatctcc aacaagcagc taaccgatgt gctggcggag tttgagcaag aagctgaaca
     3901 gtttgccgca ctgaatatca acgccaacga ttatctgcgt tcggtattgg tcaaagctct
     3961 gggtgaggaa cgtgccgcca gcctgctgga agatattctc gaaactcgcg ataccgccag
     4021 cggtattgaa acgctcaact ttatggagcc gcagagtgcc gccgatctga ttcgcgatga
     4081 gcatccgcaa attatcgcca ccattctggt gcatctgaag cgcgcccagg ccgccgatat
     4141 tctggcgttg ttcgatgaac gtctgcgcca cgacgtgatg ttgcgtatcg ccacctttgg
     4201 cggcgtgcag ccagccgcgc tggcggaact gaccgaagta ctgaatggct tgctcgacgg
     4261 tcagaatctc aagcgcagca aaatgggcgg cgtgagaacg gcagccgaaa ttatcaacct
     4321 gatgaaaact cagcaggaag aagccgttat taccgccgtg cgcgaattcg acggcgagct
     4381 ggcgcaaaaa attatcgacg agatgttcct gttcgaaaat ctggtggatg tcgacgatcg
     4441 cagcattcag cgtctgttgc aggaagtgga ttccgaatcg ctgttgatcg cgctgaaagg
     4501 ggccgagcag ccactgcgcg agaaattcct gcgcaatatg tcgcagcgtg ccgccgatat
     4561 tctgcgcgac gatctcgcca accgtggtcc ggtgcgtctg tcgcaggtgg aaaacgaaca
     4621 gaaagcgatt ctgctgattg tgcgccgcct tgccgaaact ggcgagatgg taattggcag
     4681 cggcgaggat acctatgtct gataatctgc cgtggaaaac ctggacgccg gacgatctcg
     4741 cgccaccaca ggcagagttt gtgcccatgg tcgagccgga agaaaccatc attgaagagg
     4801 ccgaacccag ccttgagcag caactggcgc aactgcaaat gcaggcccat gagcaaggtt
     4861 atcaggcggg tattgccgaa ggtcgccagc aaggtcataa gcagggctat caggaaggac
     4921 tggcccaggg gctggagcaa ggtctggcag aggcgaagtc tcaacaagcg ccaattcatg
     4981 cccggatgca gcaactggtc agcgaatttc aaactaccct tgatgcactt gatagtgtga
     5041 ttgcttcgcg cctgatgcag atggcgctgg aggcggcacg tcaggtcatc ggtcagacgc
     5101 caacagtgga taactcggca ctgatcaaac agatccaaca gttgttgcag caagaaccgt
     5161 tattcagcgg caaaccgcaa ctacgcgtgc accccgatga tctgcaacgt gtggatgata
     5221 tgctcggcgc aaccttaagt ttgcatggct ggcgcttgcg gggcgatccc acactccatc
     5281 ctggcggctg taaagtctcc gccgatgaag gcgatctcga cgccagtgtc gccactcgct
     5341 ggcaagaact ctgccgtctg gcagcaccag gagtggtgta atgaccacgc gcctgactcg
     5401 ctggctaacc acgctggata actttgaagc caaaatggcg cagttgcctg cggtacgtcg
     5461 ctacgggcga ttaacccgcg ctaccgggct ggtgctggaa gccaccggat tacaattgcc
     5521 gctcggcgca acctgtgtca ttgaacgcca gaacggcagc gaaacgcacg aagtagaaag
     5581 cgaagtcgtt ggctttaacg gtcaacggct gtttttaatg ccgctggagg aagtggaagg
     5641 tgtcctgccc ggcgcgcggg tttatgccaa aaacatttcg gctgaaggac tgcaaagcgg
     5701 caagcagtta ccgctcggtc cggcgttatt aggtcgtgtt ctggacggca gcggtaaacc
     5761 gctcgatggc ctcccctccc ccgatacgac ggaaaccggt gcgctgatta ccccgccatt
     5821 taacccgttg caacgtacac cgattgaaca tgtgctggac accggcgtgc gcccaatcaa
     5881 tgccctgctt accgttgggc gtgggcagcg tatggggctg tttgccgggt ccggcgttgg
     5941 taaaagtgtg ctgctgggga tgatggcacg ttacacccgc gccgatgtca ttgtcgtggg
     6001 tttgattggt gaacgcggac gcgaagtaaa agattttatt gagaatatcc tcggtgccga
     6061 agggcgtgca cgctccgtgg tgattgccgc tccggcggat gtttctcctc tcctgcgaat
     6121 gcagggtgcc gcctatgcca cgcgcattgc cgaagatttt cgcgatcgtg gtcagcatgt
     6181 gttgctgatt atggactccc tcacccgcta cgcgatggcc cagcgtgaaa ttgcgctggc
     6241 gattggcgaa ccacccgcca ctaaaggtta tcccccgtcg gtgtttgcca aattaccggc
     6301 actggtcgag cgtgccggaa atggcattag cggcggcgga tcgattactg cattttatac
     6361 cgtgctcact gaaggcgatg accagcagga ccccattgcc gactctgcgc gggcaatcct
     6421 cgacggtcac attgtgctgt ctcgccgact ggcggaagcc gggcactatc cggctatcga
     6481 tattgaagcg tcgatcagcc gcgcaatgac ggcgttgatc agtgagcaac attacgcgcg
     6541 agtgcgcacc ttcaaacagc tgttgtcgag ttttcagcgt aaccgcgatc tggttagcgt
     6601 cggcgcgtat gccaaaggca gcgatccgat gctcgacaaa gccatcgccc tgtggccgca
     6661 gctggagggc tatttgcaac aaggcatttt tgaacgcgcg gactgggaag cgtctctccg
     6721 ggggctggag cgtattttcc cgacagtgtc ataacccagg agataacggc agatggcaga
     6781 acatggcgcg ctggcgaccc tgaaagatct ggcagaaaaa gaggtagagg atgccgcgcg
     6841 cctgctgggt gaaatgcgtc gcgggtgtca gcaggcggaa gaacagctca aaatgctgat
     6901 cgattatcag aatgaatatc gcaataacct caacagcgat atgagtgccg ggatgaccag
     6961 caaccgctgg atcaactatc agcagtttat ccagacgctg gaaaaagcca ttactcagca
     7021 tcgccagcaa cttaatcagt ggacgcagaa agttgacatt gccctgaaca gctggcgaga
     7081 aaagaaacaa cgtttgcagg cctggcagac actgcaggaa cggcaatcca cggcggcact
     7141 gcttgcagaa aaccgcctcg atcagaaaaa gatggatgag ttcgcccagc gcgccgccat
     7201 gaggaaacct gaatgattcg cttagcgccc ttaattaccg ccgacgttga caccaccaca
     7261 ttgcctggcg gcaaagccag cgatgctgca caagattttc tcgcgttgtt gagcgaagca
     7321 ttagcaggcg agacaactac cgacaaagcg gccccccagt tgctggtggc aacagataag
     7381 cccacgacaa aaggcgagcc gctggtcagc gagattcttg ccgatgcgca acaagcggat
     7441 ttactgatcc ctgtggatga aacaccgcct gtcatcaacg acgaacaatc cacatcaaca
     7501 ccattaacca cggctcaaac gatgacgttg gctgcggtgg ctggcaacaa tacggcaaaa
     7561 gacgaaaaag cggatgatct gaatgaagac gtcaccgcaa gcctgagcgc cctttttgcg
     7621 atgttgccgg gttttgacaa tacgcccaaa gtgactgatg tgccgtcaac cgtgttaccg
     7681 gcagagaaac caacgctctt cacaaaactg acttctgcgc aactcacaac agcacagcct
     7741 gatgatgccc ccggcacgcc agctcagcca ttaacaccgc tggtagcaga agcccagagt
     7801 aaagcggaag tcatcagcac accttcaccg gtgaccgctg ccgccagccc gctaatcact
     7861 ccacaccaga cacagccact gcccaccgtc gccgcgcctg tgttgagtgc accgctgggt
     7921 tctcacgaat ggcaacaatc attaagccag catatttcgc tgttcacccg ccaggggcaa
     7981 caaagtgcag agttgcgtct gcacccgcag gatttaggtg aagtgcaaat ctccctcaaa
     8041 gtggatgata accaggcgca aatccagatg gtttcaccgc atcaacacgt acgcgccgcc
     8101 ctggaagcag cgcttccggt actgcgaacg cagctggccg aaagtggcat tcagttaggg
     8161 caaagcaaca tcagtggcga aagctttagt ggtcagcagc aggccgcttc ccagcaacag
     8221 caaagccaac gcacagcaaa ccatgaacct ctggcggggg aagacgacga tacgcttccg
     8281 gttcccgtct ctttacaagt acgtgtaaca ggcaacagcg gcgttgatat tttcgcctaa
     8341 cgtcagaggt agcaccgtaa tccgcgtctt ttccccgctt tgttgcgctc aagacgcagg
     8401 ataattagcc gataagcagt agcgacacag gaagaccgca acacatgact gattacgcga
     8461 taagcaagaa aagcaagcga tcgctttgga tcccgattct ggtattcatt accctcgcgg
     8521 cctgtgccag cgcaggttac agctactggc attcgcatca ggttgccgct gacgacaaag
     8581 cgcagcaacg cgtcgtgccc tccccggtct tctatgcgct ggataccttt acggtcaatc
     8641 tgggcgatgc agatcgcgta ctttatatcg gcataaccct gcgcctgaaa gatgaagcta
     8701 cccgctcgcg actgagtgag tatttgccgg aagtccgtag ccgtttgctg ttactgtttt
     8761 cgcgtcagga tgctgccgta ctggcgacag aagaaggcaa gaaaaacctg attgccgaga
     8821 ttaaaaccac gctttccacc ccgcttgttg ccgggcaacc gaaacaggat gtcaccgacg
     8881 tgctgtatac cgcttttatt ctgcgataac gacatgggcg atagtattct ttctcaagct
     8941 gaaattgatg cgctgttgaa tggtgacagc gaagtcaaag acgaaccgac agccagtgtt
     9001 agcggcgaaa gtgacattcg tccgtacgat ccgaataccc aacgacgggt tgtgcgcgaa
     9061 cgtttgcagg cgctggaaat cattaatgag cgctttgccc gccattttcg tatggggctg
     9121 ttcaacctgc tgcggcgtag cccggatata accgtcgggg ccatccgcat tcagccgtac
     9181 catgaatttg cccgcaacct gccggtgccg accaacctga accttatcca tctgaaaccg
     9241 ctgcgcggca cagggctggt ggtgttttca ccgagtctgg tgtttatcgc cgtggataac
     9301 ctgtttggcg gcgatggacg cttcccgacc aaagtggaag gtcgcgagtt tacccatacc
     9361 gaacagcgcg tcatcaaccg catgttaaag ctggcgcttg aaggctatag cgacgcctgg
     9421 aaggcgatta atccgctgga agttgagtac gtgcgttcgg aaatgcaggt gaaatttacc
     9481 aatatcacca cctcgccgaa cgacattgtg gttaacacgc cgttccatgt ggagattggc
     9541 aacctgaccg gcgaatttaa tatctgtctg ccattcagca tgatcgagcc gctacgggaa
     9601 ttgttggtta acccgccgct ggaaaactcg cgtaatgaag atcagaactg gcgcgataac
     9661 ctggtgcgcc aggtgcagca ttcacagctg gagctggtcg ccaactttgc cgatatctcg
     9721 ctacgcctgt cgcagatttt aaaactgaaa cccggcgacg tcctgccgat agaaaaaccc
     9781 gatcgcatca tcgctcatgt tgacggcgtt ccggtactga ccagtcagta cggcaccctc
     9841 aacggtcagt atgcgttacg gatagaacat ttgattaacc cgattttaaa ttctctgaac
     9901 gaggaacagc ccaaatgagt gacatgaata atccggccga tgacaacaac ggcgcaatgg
     9961 acgatctgtg ggctgaagct ttgagcgaac aaaaatcaac cagcagcaaa agcgctgccg
    10021 acgcggtgtt ccagcaattt ggcggtggtg atgtcagcgg aacgttgcag gatatcgacc
    10081 tgattatgga tattccggtc aagctgaccg tcgagctggg ccgtacgcgg atgaccatca
    10141 aagagctgtt gcgtctgacg caagggtccg tcgtggcgct ggacggtctg gcgggcgaac
    10201 cactggatat tctgatcaac ggttatttaa ttgcccaggg cgaagtggtg gtcgttgccg
    10261 ataaatatgg cgtgcggatc accgatatca ttactccgtc tgagcgaatg cgccgcctga
    10321 gccgttagtg atgaataacc acgctactgt gcaatcttcc gcgccggttt ctgctgcgcc
    10381 actgctgcag gtgagcggcg cactcatcgc cattattgcc ctgatcctcg ctgctgcctg
    10441 gctggtaaaa cggttgggat ttgcccctaa acgcactggc gttaacggtc tgaaaattag
    10501 cgccagtgct tcgctgggcg cgcgtgaacg ggttgtggtg gtcgatgtgg aagatgcacg
    10561 gctggtgctc ggcgttaccg cagggcaaat caatctgcta cataaacttc ccccttcagc
    10621 accaacggaa gagataccgc agcccgattt tcagtcggtc atgaaaaact tgcttaagcg
    10681 tagcgggaga tcctgatgcg tcgtttgttg tctgtcgccc ctgtccttct ctggctgatt
    10741 acgcccctcg ccttcgcgca actgccgggt atcaccagcc agccgctgcc tggcggtgga
    10801 caaagctggt cgctcccggt gcagacgctg gtgttcatca cctcgttgac gtttattccg
    10861 gcaattttac tgatgatgac cagtttcacc cgcatcatta ttgtttttgg tttattgcgt
    10921 aacgcgctgg gaacaccctc cgcgccacct aaccaggtat tgctggggtt ggcgctgttt
    10981 ttgacctttt ttattatgtc gccggtgatc gacaaaattt atgtcgatgc gtaccagcca
    11041 ttcagcgaag agaaaatatc aatgcaggag gcgctggaaa aaggggcgca gccgctgcgt
    11101 gagtttatgc tgcgtcagac ccgtgaggcg gatttagggt tgtttgccag actggcgaat
    11161 actggcccgt tgcagggacc tgaagccgtg ccgatgcgca ttttgctccc ggcctacgtg
    11221 accagcgagt tgaaaaccgc atttcagata ggcttcacga ttttcatccc ttttttgatt
    11281 atcgacctag tgatagccag cgtgttgatg gcattaggga tgatgatggt tccccccgcc
    11341 accattgctc tgccctttaa actgatgctg tttgtactgg tggatggctg gcaattgctg
    11401 gtcggttcgc tggcgcagag cttttacagc tagagaggca aaatgacacc tgaatcggtc
    11461 atgatgatgg ggactgaagc gatgaaagtc gcgctggcac tggctgcccc gctattgttg
    11521 gtagcgttgg tcacgggcct tatcatcagt attttgcagg ccgccaccca gattaacgaa
    11581 atgacgctgt cgtttattcc gaaaatcatc gccgtattta ttgccattat tattgccgga
    11641 ccgtggatgc tcaatctgtt gctggattac gtccgcacct tgttcactaa cctgccgtat
    11701 atcatcgggt agccgtacta tgttgcaggt gacaagcgat caatggcttt cctggctaag
    11761 tctgtacttc tggccgttac tgcgcgtgct ggcgctcatt tctaccgcgc caattctgag
    11821 cgaacgcagc gtgccgaaac gggtaaaact gggtctggca atgatgatca cgttcgctat
    11881 tgccccatca ttacctgcca acgatgttcc tgttttttcg ttctttgctc tgtggctggc
    11941 cgtgcagcag atcctgatcg gcattgcgct tggtttcacc atgcaatttg cctttgccgc
    12001 tgtgcgaacc gctggcgaaa ttatcggtct gcaaatgggg ctgtcatttg cgacgtttgt
    12061 cgatccggcc agccatctca acatgcccgt tctagcgcgt atcatggata tgctggcgtt
    12121 actgctgttc ctgacattta acggtcattt atggttgatt tcactgctgg tcgatacctt
    12181 tcacaccctg ccgattggtg gcgaaccgtt gaacagcaat gcgtttctgg cactcaccaa
    12241 agcagggagt ttgattttcc ttaacgggct gatgctggcg ttaccgctca ttactctgct
    12301 gctgaccctg aatctggcat taggtttact gaatcgtatg gccccgcaat tatccatttt
    12361 tgttattgga tttccattaa ctctgactgt cggtatctct ttaatggcgg cattaatgcc
    12421 gttaattgcg cctttttgcg aacatttatt cagtgagatg tttaatttgc tggctgatat
    12481 tattagtgaa ttgccattaa tataattccg taacgtttat catgttatcc taaggattat
    12541 ccgaaaaata atacctacga acatcttcca ggatactcct gcggcgaaat atttgtttta
    12601 agctcactca catatcgcaa catttacttt actttaagac aattccaggc aaattataca
    12661 acactttacg ggatagtaag tccgcctgaa aaatcgcgag agtggcgcat taggtgaccc
    12721 atgttgttcc gtttagtcat gatgaaatat tcaggtaagg ggaattatcg ttacgcattg
    12781 agtgagggta tgccatgtca acgattatta tggatttatg tagttacacc cgactaggtt
    12841 taaccgggta tctgttgagt agaggggtta aaaaaagaga aatcaacgac attgaaaccg
    12901 ttgatgacct tgccatagct tgtgattcac agcgcccttc agtggtgttt attaatgagg
    12961 actgtttcat ccatgatgct tctaacagtc agcacatcaa gcacatcatt aatcaacatc
    13021 ccaatacgtt atttatcgtt tttatggcaa ttgccaatgt tcattttgat gaatatctat
    13081 tggtcagaaa aaatttattg ataagttcta aatcgattaa accggaatct ctcgacgata
    13141 tccttggcga tattctgaaa aaagagacaa cgataacctc ttttttaaat atgccgacgt
    13201 tatcattgag ccgaaccgaa tcgagtatgt tgcgaatgtg gatggcaggt cagggaacca
    13261 ttcaaatctc tgaccaaatg aatatcaaag ccaagaccgt ttcatcgcat aaaggtaata
    13321 ttaaacgtaa gatcaaaacg cataataaac aggttatcta ccatgtcgtc cgactgacgg
    13381 ataatgtggc taatggtatt tttgtcaaca tgcgctaaca cattctgact ggtggtttcc
    13441 caccagtcag gctgaataag attactctgc tttctccaca aagataccgt cctgatgccc
    13501 tgcttcatta aagaaccaga tgccgagcgg gtagtcttcc agcgaaacca ggtacattgt
    13561 gccttcacta aactcctcaa ctgccagtac cacgccagga cgacgcggac cgccatccgt
    13621 tttgactgtt acccgatcat tcaccttcat cgttttcctc ctgtggcttt gtgccagtgt
    13681 agaacaattt cgtttttctg gcagcgccag gcgcgcgcga gtgctgattt tctcgacggt
    13741 ctatacttaa gagatgccag gcggacttaa cgactggcgg caacaacaga gtaacggttg
    13801 cgaggaaaga tgatgaaaac cgcaaaagag tacagcgata ccgcaaaacg tgaggtcagc
    13861 gtcgatgtcg atgccctgct ggcggcgatc aatgaaatta gcgaaagcga agttcatcgc
    13921 agccagaacg attctgaaca cgttagcgtc gatggacgtg aatatcatac atggcgtgaa
    13981 ttggcggatg ccttcgaact ggatattcat gacttcagcg tctctgaagt gaatcgttga
    14041 atgcacaata aaaaaatccc gaccctgagg gggtcgggat gaaacttgct taagcaagaa
    14101 gcacttaaaa aattcgttac accaggaaat ctgatgtgtt catcacctta tccgcaattt
    14161 ttttcgctga caagaaaata ttcgcgatat gaatgattaa cgctacttat gaatagaaat
    14221 gtgacgtgcg tcaccccttg gtggctctgg cgtcaacggg atcgctcccg aaaaaatcat
    14281 ctttgattat ttgtgctgtt ttgagaatca tcctggtaag gggagaactg cgcgaccatg
    14341 ttttcaattc aacaaccact actggttttt agcgatcttg atggcaccct gctggacagt
    14401 catagttatg actggcaacc ggctgccccc tggctcagcc gtttacgcga agcgaatgtt
    14461 cccgtcattc tctgtagcag taaaacttca gcggaaatgc tgtacttgca aaaaacgttg
    14521 gggctacaag ggttaccgct gattgccgaa aatggcgcag tgatccagct tgctgaacaa
    14581 tggcaggata tagacggttt tccacgcatc atctcaggta ttagccatgg cgaaatcagc
    14641 caggttttaa atacgctacg cgagaaagaa cattttaaat tcacgacttt cgatgatgtc
    14701 gacgatgcaa ccatcgccga atggacggga ttaagccgta gccaggcggc gctgacgcag
    14761 ctgcatgagg cgtcggtaac gctaatctgg cgcgacagtg acgagcgtat ggcacaattt
    14821 accgctcgtc tgaacgaact gggcttacag tttatgcagg gtgcgcgctt ctggcacgtc
    14881 ctggatgcct ctgccggaaa agatcaggcc gccaactgga ttatcgcgac ctatcaacaa
    14941 ttatcaggca aacgcccaac cacacttggc ctgggcgatg ggccaaacga tgcgccctta
    15001 ctggaggtaa tggattacgc ggtgattgtg aaagggctaa accgtgaagg ggtgcatctg
    15061 catgatgagg atccgacccg cgtctggcga acgcagcgtg aaggaccgga aggctggcgt
    15121 gaagggctgg accatttttt ctccgctcgt taagcgttat cgctcgcgca tacccgatta
    15181 cgcccggcct gtttagcgag ataaagccga cggtcagcca gtgactggag ttgttcaaaa
    15241 tcataatcac cggtttcctc gctgctactt acccccagcg aggcactgat gcgtatcgtc
    15301 gtactcttgg cgatcaacat ctctttttca tttagcttca ggcgaatacg ttctgcgacc
    15361 tccgcagcct ccgtcagact cgcgcctggc agaatcacac aaaactcctc accaccgacc
    15421 cgcccggcaa cgtcctgcgc acgcaaggaa ttgctaatta atcctgcagc atgagaaaga
    15481 acacggtcgc ccgcctgatg accaaagcgg tcattaatcg ctttaaaatg gtcaagatcg
    15541 acctgaatga cagaaaaagg atgttggtgc gtctgacaca atttagcgag cggacgggct
    15601 ttttcgaaca gtgcgccacg attatataaa cgggttaagg tgtcgtgcca cgcctgccac
    15661 tgcaacgagc tttgcagaac atacatgttg ctgaccatcc ggcgaatcac ataccaggag
    15721 atgagtaaca tggtggtaaa gagcgcccac agcagggtta atgcaatgct gatactgccg
    15781 aaatcgccgc gcacgccttc gcttagcgta tggacgcgca ccagcacacc gtcgaaatgg
    15841 tccagacgtt cccaactaac atagcgactg tccatacgaa tgccgccccg cgtgtcatgt
    15901 tccattgcct gcgccagcaa ggccagttca cgaggatcaa aaatattccc tgttggatga
    15961 tcaggattgg aagaggtcaa aaatctcagc ttactgtcat agagctgata ctcaccatcg
    16021 aggtttttat cgatggcgtt tcttaaaaat tgctgcatgg tacgcacggg aatactcatc
    16081 cccagcacgc cataccagta gttattactg tctaccggaa cactgacggt aacctgcggt
    16141 tcagtattgc tggcgtgttc cggttgcgaa gtaaaccagc gtaccgcgcg gtgacgattt
    16201 tctcgttgcg aatggccgat aaaccagggt tgggtgacat agctgtaata acgcgttggt
    16261 acattgcgcg taaacaaggt cggctgcgtc gaaacgtaaa atccggcacg caagacatac
    16321 atcgcctgtt caaccatcga cgaggtgttg tgcgccagtc gcagcaagta accaacttcc
    16381 agtgcagcgg taatttcatt gtcgaggctt tcattttcgc gagacaggag attcccctcg
    16441 ctgactaatg catccgacac gccattgact ggcagggtgc gtcgtcggtt gagttcgatt
    16501 tgccaggcgt gctcgtcgcg atgctgttcg aactcggtta cagcattacg cagagaggtg
    16561 aaatccagtg gcgctacgag agcttcgcgc atgccattac gcaaaaagat cagtttgtcg
    16621 acgttatact gcaaatgctt atcgagcgcg ttggctacgt tttccagatg attacgctgg
    16681 ctggagatat aggcatcttc cagcaccact acttcacgcc aggtgagcaa ggtggaaaaa
    16741 agcaatacca cgataaagca gagattaacg acatgaccag gccccaggcg gcgtgcgagt
    16801 tttttcaacc agctctggtt ttccattttt gtctcgtgct gcaccctgac gactcccaac
    16861 ctgctttttt atgattctgg cataacctgg cgatagcgta ccgcaaaaca gcgataattt
    16921 tataatggtg gcatgataag acgcgacaga catcgatagt tttataacgt aggtatgata
    16981 agacgcgata gcgtcgcatc aggcaattca gtgcagacac tggcacaccc gaccggatgc
    17041 gacgtgagcg ccacatccgg tcgggtgtta atgataccct ttcaaacttc ttccgcagga
    17101 cgactcccct cccctggaac aagctcggtt tcatgaaaag cttcccgctt gaccccgtaa
    17161 ccgtcatacc aacgacactc aaccataccg ctggagtatc cagtgacaat catccgcggg
    17221 ccgccctctt taaccgtaac ttcctcacta accataaagc tcatactcgc ctcctttttt
    17281 cgagtgaaac ttgttcacct tagttgaaga tagcgaattt tgcataatag cctgtgcgag
    17341 tattaatgtg cctgaccgcg cattttcgct acggctttca ctcccagcac cacgatgccg
    17401 ccgatgataa atccaagaat cagatttaaa acagtcggta atatcatcgc caccactgca
    17461 ctttgctgcc cggcgaaatg ttcaatggcg tgatgcagcg gcgcaatacc atgtaccaca
    17521 atcccgccgc cgacgaggaa catcgccagc gtgccgacaa tcgataacgc tttcatcagc
    17581 cagggcgcaa tgatcaataa ccctttacct aatgcctgca tcagcgcgct ggatttttcc
    17641 gccagccaat accccaggtc atcaatctta acgataaccc ctaccagacc gtagacgccc
    17701 acggtcacta ccagcgcgat gcctgaaagc accagcacct gattaagcaa cggcgcttcg
    17761 gccacaatcc ccagcgtgat ggcgacgatt tcggcagaca atataaaatc ggtacgaatc
    17821 gcccctttta ttttgtcctt ttcaaacttc agcggatcct gcgccgccag cttctccaga
    17881 cgctgctgac tctgcgccgg atcttcttta tgtttacgcg cctccagcat atgcagcact
    17941 ttctctaccc cttcaaagca gagaaacgcg ccaccaatca tcaacagagg cgtaatcgcc
    18001 cacgggataa acgcactgat gatcagcgcc agtggcacca gaatcacttt attaatcagc
    18061 gatcctttcg ccacgcccca gaccacggga agttcccggt tggcccgcac gcctgaaact
    18121 tgttgcgcat tgagcgataa gtcatcccct aatacaccgg cggttttctt cgccgccagt
    18181 ttgcccatca cggagatatc gtccagtagt gtggcgatat catcgagcaa cgtcagtaaa
    18241 ctactcccgg ccaaaagcat tccctctcat ttttattgtt gaagggtgta agtatgaagc
    18301 aaaaacggca gctctgatac ttgcttcaca ggtcaacatt tttttaacaa tcaagtgcaa
    18361 ttatatctct cgccagcaga atgattttca cgtttactat gaaccacctt tttatttcca
    18421 ccgtgaggaa ttatgcgttt ccgccagttg ttaccgcttt ttggcgcgct gtttgcgttg
    18481 tatatcattt ggggctcaac ctattttgtc attcggattg gcgtggaaag ctggcctccg
    18541 ttaatgatgg cgggcgttcg attcctggca gccggtattt tattgctggc atttttgcta
    18601 ctgcgcggac acaaactccc cccgctacgt ccgctgctca atgccgcgct gattggcctg
    18661 ttattgctgg ctgtcggtaa tggcatggtg acggttgccg agcatcaaaa tgttccttcc
    18721 ggcatcgccg ccgttgtggt tgcaaccgtg cccctcttta ccctgtgctt cagccgcctg
    18781 tttggcatta aaacgcgcaa actggaatgg gtgggtatcg ccattgggct tgccggaatc
    18841 atcatgctca atagcggtgg aaatttaagc ggcaatccgt ggggcgcgat tctgatttta
    18901 atcggctcga ttagctgggc gtttggctcg gtttatggct cgcgcattac cttacctgta
    18961 gggatgatgg cgggtgcgat tgagatgctg gcggcaggcg tggtgttaat gatcgcgtcg
    19021 atgattgcag gtgaaaaact gacggcgctc ccttcccttt caggcttcct tgcggtcggc
    19081 tatctggcgc tgtttggttc gattatcgcc attaacgctt atatgtattt aatccgcaac
    19141 gtcagtccgg ctctcgccac cagctacgcc tacgttaacc cggtggtcgc ggtcttgctg
    19201 ggtacgggac tgggcggaga aacgctgtcg aagattgaat ggttggcgct cggcgtgatt
    19261 gtcttcgcgg tggtactggt cacgttggga aaatatctct tcccggcaaa acccgtagtt
    19321 gcgccagtta ttcaggacgc atcaagcgag taaatgaatc ccctgcgtgt cgatctgcgc
    19381 gctggcacct tcgccgcaga tccactcttc cagacgctcg ctaagcgctt catccgtcag
    19441 cttctcgcgc ccacgtaacg cacactccca gacaatcagt acgcgccagc caagctcctg
    19501 caagcgactg atatcacggc gatcccgctc aacattttta cctatcttct ccagccagaa
    19561 ctcggttcga gtcgcaggca ctttaaacag atagcagtga tgatggtgcc agaagcagcc
    19621 gtgggtaaat atcacgcaac gatattcatc aacgacaaaa tccgggcgtc cgggcagact
    19681 ggcatcctga acgcgaaacg ccaggccctg cccggttaac agactggcga ggcgcttctc
    19741 aatcgccgta tcacgcgtgg caatcgcgcg catatttttg ctgcgagtgg ccttatcgtg
    19801 aacgtcggcc atgttgtgcc tcttgctgac gcaacgccac cgcctgtttg atttttggct
    19861 caagcagttt tgccaccgcg gcaaagaccg gcacgaccac cgagttaccg aactggcgat
    19921 aggcctgagt gtccgaaacc ggaatgcgga atttcgcttc tcccggcgct tcaaaaccca
    19981 ttaagcgcgc gcattcccga ggcgttaacc gacgtggacg atgttgctga ttcagcggat
    20041 cgtcaaagtc tttctcacct ttggccatat cccagccgcg atcgattaaa atttccgcgc
    20101 catctttgta ataacgcgca gacagcgtac gcgtgacgct ttgcggattg ttcggataaa
    20161 ccattccata accgaagccg ttaccgcgcg cctgatgttt tttcgcatat cgatagaggt
    20221 acttccacag caccggcgtc aggatatatt tcgcctcgac catcgggtcc agcagctgcg
    20281 ccagcgtcac tcgctgcgca gggaaacatt cgctgatatc acgcagggta aaatcggctt
    20341 tcagattcag atcgcgacga aaacccacca gcacgatgcg ttcacggtgc tgcggcagaa
    20401 aatgtttgcc gtcgatgatt ttcggatcgt ccggcccgtt atcttctgca tcagccacgt
    20461 catagcccag ttcgtccagc gtctgcatga tgatgcggaa cgttttaccc tggtcgtgac
    20521 ttttcaggtt tttgacgttt tcgagcacaa acatcgccgg acgacgcgcg tcgataatgc
    20581 gcaccacatc aaaaaacagc gtgccctggg tatcgcaggc aaaaccgtgc gcccgcccga
    20641 gcgagttctt tttcgatacg ccagccagcg aaaatggctg acaagggaaa ccggccagta
    20701 aaacatcgtg ttcaggaatg tgttgacgaa tatgttccgc cgccgcctca tcactcacgc
    20761 cttctttatg gctgagggtg atgtcgcgga tatcttcatt aaaatgatgc gtcgccgggt
    20821 cgcaataatg attggcttta taagtgcgta ccgcatgttt gttccattcg ctggtaaaca
    20881 cgcactgtcc gccaatcgat tcaaaaccgc gacggatgcc gccaattccg gcaaatagat
    20941 cgataaagcg aaacgcataa tgcggatgat gtgccggtgg tttgggtaac aacgtttgca
    21001 gatgggcgaa ctctttctca cttaaacgat gccatgccga gtcattcgcc agcgcacgtt
    21061 ttaaaattgc cgcgctccag tgattctcac ccacaccatt aagctgcgcc accagcgttt
    21121 taacatcata aatttgcagc agtttctcca gcattgcctg tgcggcattc ccggtgctgt
    21181 atgaatcggt tactgatata ttttcctgca tagatttaac cggccatctg agatggccga
    21241 caggttaaca taattacagc catttagggc cacgcgctta tcccgcgggt gaaaacgcat
    21301 ctatcacctt gtgatcgaca ccttcattct cccccgccag ttcagcactg agcttcgcca
    21361 taaactcgac taaaaaatgc gcgttgtgct gcgccagctg cttgcctctt acggtttgca
    21421 tggtttgcgg tagcttcagc aacttagtct ggaaatgatc cagcgcgtag cgtttatcat
    21481 caagcgggcg atgctgtgcg agcggatctt cgccatcaaa cagcgccacg cccaatgccc
    21541 ctgaaacggc aaacacgcgc gccaggccaa tcgcccccag ggcttccagc cgatccgcat
    21601 cctggacgat tttagcttcc attgttaagg gggcgatttg cgcgctgaaa ctgtgagcgg
    21661 caatggcatg acaaacggcc tcgattttct ccgccggaaa ttgcacgaac tcttcacgga
    21721 gcaggcgacg cgtctcttct gccgccagga ttgaagaatg ctgccgttgc ggatgatttt
    21781 tcgccaggct cacaatatcg tgaaaataac aggccgttaa aatcaccagc atatcgacgt
    21841 catcatctgc cgccagcttc tgtgccgtag cccagacgcg gcgaaaatga cacacgtcat
    21901 gggccgcgtc ctggtgctga tgatggtttt tcaaccagtt ttcgaactct gcctgccagt
    21961 gttgtaagtc catacgcgct ccgttgccaa agagtcgcca ccttagcagt ttttattcat
    22021 agcgtctgct acgtgcggga cgaggtcggg tgtaccaggc aatgctgcct aacaatgccc
    22081 cgaccagagc cataaataaa aatccgacca gtgcccctaa ccactttccg gcggtagagc
    22141 ctaaatcgct ttcaaatccc gacacggggg catcggggat ccggctcatc acccagatgt
    22201 aacacgccat tgcccagaca aaatagccac accatgtata gaaagcccac aaagccagtt
    22261 tgccgccagg gctgcgagag agtctctctt ccatctttgt cgaaccgtat tccattttga
    22321 gataacccgg aaaattcaga cttagtacta ttatgtgata aaagtcacat ttttccacat
    22381 tagatgagcc agtcaagact cactcatttt ttgaacaaat aga
//
LOCUS       DAPHBJ010000064        22056 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.064, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000064 DAPHBJ010000000
VERSION     DAPHBJ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.064"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            88..501
                     /locus_tag="RHZ76_002154"
     CDS             88..501
                     /locus_tag="RHZ76_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDV2812796.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(546..863)
                     /gene="hspQ"
                     /locus_tag="RHZ76_002155"
     CDS             complement(546..863)
                     /gene="hspQ"
                     /locus_tag="RHZ76_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HDV2812797.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(921..2111)
                     /gene="rlmI"
                     /locus_tag="RHZ76_002156"
     CDS             complement(921..2111)
                     /gene="rlmI"
                     /locus_tag="RHZ76_002156"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HDV2812798.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETVDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECSIYDRSDVA
                     VRKKEGMELTQGPVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            2206..2484
                     /gene="yccX"
                     /locus_tag="RHZ76_002157"
     CDS             2206..2484
                     /gene="yccX"
                     /locus_tag="RHZ76_002157"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDV2812799.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(2481..2810)
                     /gene="tusE"
                     /locus_tag="RHZ76_002158"
     CDS             complement(2481..2810)
                     /gene="tusE"
                     /locus_tag="RHZ76_002158"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HDV2812800.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(2901..3560)
                     /gene="yccA"
                     /locus_tag="RHZ76_002159"
     CDS             complement(2901..3560)
                     /gene="yccA"
                     /locus_tag="RHZ76_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HDV2812801.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(3767..3854)
                     /locus_tag="RHZ76_002160"
     tRNA            complement(3767..3854)
                     /locus_tag="RHZ76_002160"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3818..3820),aa:Ser,seq:tga)
     gene            4281..5399
                     /gene="hyaA"
                     /locus_tag="RHZ76_002161"
     CDS             4281..5399
                     /gene="hyaA"
                     /locus_tag="RHZ76_002161"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HDV2812802.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            5396..7189
                     /gene="hyaB"
                     /locus_tag="RHZ76_002162"
     CDS             5396..7189
                     /gene="hyaB"
                     /locus_tag="RHZ76_002162"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HDV2812803.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            7208..7915
                     /gene="hyaC"
                     /locus_tag="RHZ76_002163"
     CDS             7208..7915
                     /gene="hyaC"
                     /locus_tag="RHZ76_002163"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HDV2812804.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            7912..8499
                     /locus_tag="RHZ76_002164"
     CDS             7912..8499
                     /locus_tag="RHZ76_002164"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HDV2812805.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            8496..8894
                     /gene="hyaE"
                     /locus_tag="RHZ76_002165"
     CDS             8496..8894
                     /gene="hyaE"
                     /locus_tag="RHZ76_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HDV2812806.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            8891..9748
                     /locus_tag="RHZ76_002166"
     CDS             8891..9748
                     /locus_tag="RHZ76_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HDV2812807.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            9882..11426
                     /gene="appC"
                     /locus_tag="RHZ76_002167"
     CDS             9882..11426
                     /gene="appC"
                     /locus_tag="RHZ76_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HDV2812808.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFALKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRGLEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            11438..12574
                     /gene="appB"
                     /locus_tag="RHZ76_002168"
     CDS             11438..12574
                     /gene="appB"
                     /locus_tag="RHZ76_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDV2812809.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            12587..12679
                     /gene="cbdX"
                     /locus_tag="RHZ76_002169"
     CDS             12587..12679
                     /gene="cbdX"
                     /locus_tag="RHZ76_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HDV2812810.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            12759..14057
                     /gene="appA"
                     /locus_tag="RHZ76_002170"
     CDS             12759..14057
                     /gene="appA"
                     /locus_tag="RHZ76_002170"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HDV2812811.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(14172..16352)
                     /gene="etk"
                     /locus_tag="RHZ76_002171"
     CDS             complement(14172..16352)
                     /gene="etk"
                     /locus_tag="RHZ76_002171"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HDV2812812.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGIEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(16372..16818)
                     /gene="etp"
                     /locus_tag="RHZ76_002172"
                     /gene_synonym="yccY"
     CDS             complement(16372..16818)
                     /gene="etp"
                     /locus_tag="RHZ76_002172"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HDV2812813.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(16806..17945)
                     /locus_tag="RHZ76_002173"
     CDS             complement(16806..17945)
                     /locus_tag="RHZ76_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HDV2812814.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(17991..20087)
                     /locus_tag="RHZ76_002174"
     CDS             complement(17991..20087)
                     /locus_tag="RHZ76_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDV2812815.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDACWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(20087..20833)
                     /locus_tag="RHZ76_002175"
     CDS             complement(20087..20833)
                     /locus_tag="RHZ76_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDV2812816.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(20830..21474)
                     /gene="gfcB"
                     /locus_tag="RHZ76_002176"
     CDS             complement(20830..21474)
                     /gene="gfcB"
                     /locus_tag="RHZ76_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HDV2812817.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(21581..21886)
                     /locus_tag="RHZ76_002177"
     CDS             complement(21581..21886)
                     /locus_tag="RHZ76_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812818.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATKTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTRR"
BASE COUNT         5166 a         5727 c         5823 g         5340 t
ORIGIN      
        1 ccgcttttgc tctcattcat tcgtattagc tgcatggttg gcatgtcggc tttgtcgtac
       61 actttgttaa acagtctgga ggaaataatg aaagaaaccg atattgcagg tattttaacg
      121 tccactcaca ccattgcgct ggtgggggca agcgacaaac ccgatcgtcc cagctatcgc
      181 gtgatgaaat atctactcga ccaggggtat cacgtcattc cggtttcgcc aaaagttgcc
      241 ggcaaaacgc tgctgggcca gcaggggtat ggcacgctgg cagacgttcc ggaaaaagtc
      301 gatatggtcg atgttttccg taattcagaa gctgcgtggg gcgtggcgca ggaagctatc
      361 gccattggcg cgaaaaccct ctggatgcaa ctgggggtga ttaatgagca agcggcagta
      421 ctggcacggg atgccgggtt aaatgtggtg atggatcgct gcccggctat tgagatccct
      481 cgcctgggcc tggccaaata aaaaatcccc ggaaggcaaa aaccttccgg ggattcgttc
      541 agggatcagt tacgcagacg cggggcctgg agttgtttgc ggatggtctg cgccagctca
      601 tccatggatg gctgttcagg atgttcatcc tgcaattcgc tacttagctg ggcttcagcc
      661 aggtaggtat gaaccggtag gccgttatcg tcctccatca ccacgtgata ccacggagcg
      721 gcgcgaagct cgtcattcac cgccagctca tcaggcgacg gttcagagag cgaataaacc
      781 gggtcgatat ccacgaccac tccgagataa cctaacaggg aatggcggac ctgctggccg
      841 ataccgaatt tgctggcaat catagtcacc tcccgggaaa tctttacctt tgatataagg
      901 gtaaaattcc acatttcaag ttacatgacg cgacaggcaa accctttcag atatagccct
      961 tccgggtagg tagcgatcac cggatgatcg gctgcctgac ggaactgctc tataaattgt
     1021 acatcacggc cggcatcaat tgcggcatcc gcgatgattt tctgaaataa atcgctggtc
     1081 atcagaccgg aacaggagaa agtcaggaga ataccgcctt cattcagcaa ctgaatcgcc
     1141 agcatgttga tatctttata gccccgacac gcgcccatca actggctttt attctcaaca
     1201 aacttcggcg ggtccatcac gataacgtca aatttttcac cgcgatcgcg ataagtacgc
     1261 agcaatttaa agacatcatc acggacaaac tcagccttgc tcagatccag tttgttcagc
     1321 tcaacgttct gccgtgcaat atccagcgct tcctgggagg tatcaacgct gacaacctgg
     1381 ctgcaaccgc ccatcagtgc cgatacggcg aaaccaccgg tataggagaa acagttcagc
     1441 acacgtttat tttcaacgta gcggcgggta gccaggcggc tatcacgctg gtccaggtag
     1501 tagcccgttt tgtgtccgtg ctgaatatcc accagcagtt tcattccgtg ttcttcaatc
     1561 ggcagcaggg caggtggcaa ctcgccggtg acggggccct gggtcagctc cattccttct
     1621 tttttacgta ccgcgacgtc gctgcgatcg taaatcgaac attccgggta cagcgtttgc
     1681 agggcactaa ttaatgccgc gcgctgatat tctgccccag cactcagcag ttgcagcacc
     1741 agaaaattac cgaaacgatc gatagtaata cccggcaggc catcagattc tccggcgatt
     1801 aaacgatagc tgtcgaggcc atctttttgc gccagccagt cacgccattt ttgtgcttgt
     1861 tgcaaacggc gggaaaaaaa agcaatgtcg atagactcag acgggtcaaa cgtccagacg
     1921 cgcgcccgga tttgcgaagc tggcgaataa gcgccgcgtg ctaaccattt tccctgatga
     1981 tcaacaatat cgacggtttc accgaggctg gctttacctt ccatgcgggc aacggccccg
     2041 gaaaagaccc acggatggcg acgaagtaat gatttttcgc gccctttggc taacactaaa
     2101 cgtacactca taatttactt ttctgtcgat gcaaagaaat gggcgtcatt gtccggagtt
     2161 ttccatggat ttgcaacgcg tccattaagg ataaggagag cagcaatgtc gaaagtctgc
     2221 ataattgcct gggtttacgg gcgagttcag ggcgtaggat ttcgctacac cacacagtac
     2281 gaagcgaaaa gactgggttt aaccgggtac gccaaaaatc ttgacgatgg cagcgtggaa
     2341 gtggttgctt gcggtgaaga agggcaggtg gaaaaattaa tgcagtggtt aaagagtggc
     2401 ggcccgcgtt ctgcgcgtgt agaacgggtg ctcagcgagc cgcatcatcc ctcgggggaa
     2461 ttaaccgatt ttcgtatccg ttaaatacat tttaccggtt ttggcaggcc agcaattttg
     2521 gtggcttgct tagccggacc tttcggaaac agtcggtata aatagcggct gttacctttt
     2581 tcttcgccaa atttattcgc catcgctttt accagcatac gaatcgccgg agaagtattg
     2641 aattccagat agaaatcacg cacaaaacgc accacttccc agtgttccgg tgacagcgaa
     2701 atcccttcgt tctctgcaat caccacagcc agcggctcac tccactggct gctttctttg
     2761 agatagcctt cggtatccgt ttctatctct ttaccttcga agatcagcat aattattact
     2821 accttaatca gactggcggc agtgtaacaa aaaacaaagc cccgcataaa gcgaggctat
     2881 gaaagtgtta gcgggtgaga ttaatcgcgg ctagcgaagc ccagaatgct cagcaggctg
     2941 acgaagatgt tgtacagcga aacatacagg ctaaccgtgg cacgaatata gttcgtctca
     3001 ccgccatgaa tgatgttgct ggtttcaaac aagatagcgc cagaggagat cagaatgaag
     3061 accgcgctga tcgccagatg cagagcaggc agctgcagga agatattcgc aaccatacca
     3121 atcagcacca ccacaatacc cgccatcagc ataccgccga ggaacgacat atctttgcgg
     3181 gtggtcagca catatgcaga gcagcagaag aacactaacg ccgttccgcc cagtgccata
     3241 gcgattacgt cacccattcc ggcagacaga taggtgttca gaataggtcc gaggatataa
     3301 cccagaaaac cggtaaaggc gaatgcggag ataatcccgg tcggcttatt cgccgtttta
     3361 taggtcagga acatcaaacc atacataccc accagcgtca gaatcagacc cggagatggc
     3421 agcatcagca ccgtactggc agttgcggta atcgccgaaa aggccagcgt caggctcagc
     3481 agaaaatagg tattacgcag caccttatgg gtgctaagca gtgatgtacg gtcatgtgaa
     3541 gaactaacaa tacgatccat tgagtcactc tctatgacag atgtaattaa ttaagcagca
     3601 taatgataat gcgtaagggc acccagaagt tttacccatc tttacgcatt tgatctggaa
     3661 caggtttaac agcggattat caggtcatta agcaaatata acgccctgag aatttcgaca
     3721 ggcaaaagaa aaaggggtta gcatttagct aaccccttat cttatttggc ggaagcgcag
     3781 agattcgaac tctggaaccc tttcgggtcg ccggttttca agaccggtgc cttcaaccgc
     3841 tcggccacac ttccggaatg acgcgcacta taaacatccc gatgcggcgt gtaaacccct
     3901 aatttgtttg tttgcctgaa aaacagccaa aagtgcattg atagcgtgaa ataacagcag
     3961 attgatcatt tcatcaccat gaattccttc tcttttactc gtttagcaac cggctaaaca
     4021 tccccaccgc ccggccaaaa gaaaaatagg tccattttta tcgctaaaag ataaatccac
     4081 acagtttgta ttgttttgtg caaaagtttc actacgcttt attaacaata ctttctggcg
     4141 acgtgcgcca gtgcagaagg atgagctttc gttttcagca tctcacgtga agcgatggtt
     4201 tgccttgcta cagggacgtc gcttgccgac cataagcgcc cggtgtcctg ccggtgtcgc
     4261 aaggaggaga gacgtgcgat atgaataacg aggaaacatt ttaccaggcc atgcggcgtc
     4321 agggcgttac ccggcgcagc tttctcaaat attgtagtct ggctgccacg tcgctgggat
     4381 taggcgcggg aatggcacca aagattgcct gggcgctgga gaacaaaccg cgcattccgg
     4441 tggtatggat ccacggtttg gaatgcacct gctgtaccga atcttttatc cgctccgctc
     4501 acccactggc gaaggacgtc atcctttccc tgatttccct cgattacgac gatactttga
     4561 tggctgccgc cggaacccag gcggaagaag tctttgaaga catcatcacg caatacaatg
     4621 gcaaatatat cctcgcagta gaaggtaatc cgccgctggg cgagcagggg atgttctgta
     4681 tcagcagcgg tcgaccgttt attgagaaac tcaaacgtgc cgctgccgga gccagcgcga
     4741 ttatcgcctg gggaacctgc gcgtcctggg gctgcgtgca ggccgcgcga cccaatccga
     4801 cgcaggcaac gcctatcgac aaagtcatca ccgacaaacc cattatcaaa gtacctggct
     4861 gcccgccgat cccggatgtg atgagcgcca tcattactta catggtgacc tttgatcgct
     4921 tgccagatgt cgacagaatg ggccgtccgc tgatgttcta tggtcagcga atccacgata
     4981 aatgctatcg ccgcgcccac ttcgacgccg gagagttcgt ccagagttgg gatgatgacg
     5041 ctgcccgcaa aggttactgc ctgtacaaaa tgggctgcaa agggcctacc acctataacg
     5101 cctgttcctc cacacgctgg aatgatggcg tttctttccc aatccagtct ggtcacggct
     5161 gcctgggctg tgcggaaaat ggtttctggg atcgcggttc gttctacagc cgcgtggtcg
     5221 atattccgca aatgggtact cattccaccg ccgataccgt cggtttaacc gcgcttggcg
     5281 tggtggcagc ggctgttggt gtgcacgcag tcgccagcgc cgttgaccag cgcagacgtc
     5341 ataaccagca acctacagaa accgaacatc agccaggcaa tgaggataaa caggcatgag
     5401 cactcagtac gaaactcagg gatacaccat caataatgcc ggacgccgcc tggtggtcga
     5461 cccgattacg cgcatcgaag gccacatgcg ctgcgaagtg aatattaacg atcagaatgt
     5521 gatcaccaat gccgtctcct gcggcaccat gtttcgcggg ctggagatca tcctacaagg
     5581 gcgcgacccg cgcgatgcgt gggcgttcgt tgaacgtatc tgcggcgtct gtactggcgt
     5641 acacgccctg gcttcggttt acgccatcga agatgctatc ggtattaaag tgccggacaa
     5701 cgccaatatc atccgcaaca ttatgctggc aacgctctgg tgccacgatc atctggtgca
     5761 cttctatcag cttgccggga tggactggat cgatgtgtta gatgcgctga aagccgaccc
     5821 gcggaaaacc tccgaactgg cgcaaagtct ctcctcttgg ccgaaatcat cccctggcta
     5881 tttcttcgac gtacaaaacc gcctgaaaaa atttgttgaa ggcgggcagt tggggatctt
     5941 ccgcaatggc tactgggggc acccgcagta caaactgccg ccagaagcta acctgatggg
     6001 ctttgcccac tatctcgaag ctctcgattt ccagcgtgaa attgtcaaaa tccacgcggt
     6061 ctttggcggt aaaaacccgc atccaaactg gattgtcggc gggatgcctt gcgccatcaa
     6121 cattgacgaa agcggcgcgg tcggggcagt caatatggaa cgcctgaacc tggtgcagtc
     6181 aattatcacc cgcacggcgg acttcattaa caacgtgatg atccccgacg ccttagccat
     6241 cggtcagttc aacaaaccgt ggagcgaaat cggcactggt ctttctgata aatgcgttct
     6301 cagctacggc gcattcccgg atattgccaa cgactttggc gagaaaagtc tgctgatgcc
     6361 tggcggcgcg gtgattaacg gcgacttcaa caatgtgctg ccagtggatt tggttgatcc
     6421 gcagcaggtg caggagtttg tcgaccacgc ctggtatcga tatcccaacg atcaggtcgg
     6481 gcgtcatccg ttcgatggca tcaccgaccc gtggtacaac cccggcgatg tcaaaggcag
     6541 cgataccaac attcagcagc tgaatgaaca ggaacgctac tcgtggatca aagcgccacg
     6601 ctggcgcggt aacgcgatgg aagtggggcc gctggcgcgc acgttaatcg cttatcacaa
     6661 aggcgatgct gcgaccgttg agtcggtcga tcgcatgatg tcggcgttga acctgccgct
     6721 ttccggtatc cagtcaacgt taggccgcat tttgtgccgc gcgcacgaag cgcagtgggc
     6781 cgcaggtaag ttgcagtatt tcttcgacaa gctgatgacc aacctgaaaa acggcaatct
     6841 cgccactgct tccacggaaa aatgggaacc tgcaacctgg ccgacagagt gccgtggtgt
     6901 cggttttacc gaagcgccgc gcggggcgtt aggccactgg gccgccattc gcgatggcaa
     6961 gattgatctc taccagtgcg tggtgccgac cacctggaac gccagcccgc gcgatcccaa
     7021 agggcagatt ggcgcttatg aagcggcgct gatgaacacc aaaatggcga tccccgagca
     7081 accgctggag atcctgcgta ctctgcacag ctttgacccg tgcctcgcct gttcaacaca
     7141 cgtgctgggc gacgacggta gcgagctgat ctccgtgcag gtgcgttaac agcgaaggag
     7201 aatcatcatg caacagaaaa gcgacaacgt tgtcagccac tatgtctttg aagcgccagt
     7261 gcgcatctgg cactggttga cggtgttatg catggcggtg ttgatggtca ccggatactt
     7321 tatcggcaag ccgctacctt ccgtcagcgg cgaggcgacg tatctgttct atatgggcta
     7381 catcaggtta attcacttca gcgccgggat ggtttttacc gtggttttgc tgatgcggat
     7441 ctactgggct tttgttggca atcgatactc ccgcgagctg tttatcgtgc cggtatggcg
     7501 taaaagctgg tggcagggcg tgtggtatga aatccgctgg tatctgtttc tggcaaaacg
     7561 tccgagtgcc gatataggcc ataatcccat cgcccaggcg gcgatgttcg gctatttcct
     7621 gatgtcggtc tttatgatca tcactggttt tgcgctgtac agcgaacaca gccagtacgc
     7681 tatttttgcg ccgttccgtt atgtggtgga atttttctac tggacgggtg gcaactcaat
     7741 ggacattcac agctggcatc ggctggggat gtggctgatt ggcgcgtttg tgatcggtca
     7801 tgtctacatg gcgctgcgtg aagacatcat gtccgacgac acggtgatct ccaccatggt
     7861 caacggctac cgtagccaca aatttggcaa aataagtaac aaggagcgtt catgagcgag
     7921 caacgcgtgg tggtcatggg gctgggcaac ctgctgtggg ccgatgaagg cttcggcgtg
     7981 cgggtggcgg aacggctgta tgcccattac cactggcccg agtatgtgga gattgtcgat
     8041 ggcggtactc agggactgaa cttgctgggg tatgtcgaaa gcgccagcca tctgttgatt
     8101 ctcgatgcca ttgactacgg gctggaacct ggaacgctgc gaacctatgc cggagaacgc
     8161 attccggctt atctcagcgc gaagaaaatg agcctgcatc agaacagttt ctccgaagtg
     8221 ttggcgctgg cggatatccg cggacatctg ccagcacata ttgccctcgt cggtctgcaa
     8281 cccgcaatgc tcgacgacta cggcggtagc ctgagcgaac tggcacggga gcaactgccc
     8341 gctgcggaac aggcggcgct ggcgcagctt gctgcgtggg gaattgtgcc gcaaccggct
     8401 aatgaatcgc gctgtctcaa ttatgactgt ctgtcgatgg aaaattacga aggcgttcgc
     8461 ttgcgccagt accggatgac acaggaggag cagggatgag caacgacacg ccatttgatg
     8521 cgttgtggca acgaatgctg gcgcgcggct ggacgccagt cagtgaatcc cgtcttgacg
     8581 actggcttac gcaagcgcca gacggcgtgg tgttattaag cagtgacccg aaacgcacgc
     8641 cagaggtcag cgataatccg gtaatgattg gcgaattact gcgcgagttt cccgactata
     8701 catggcaggt ggcgattgct gaccttgagc agagcgaagc catcggcgat cgctttggcg
     8761 tctttcgctt tcctgccact ttagtgttta ccggcggaaa ctatcgcggc gtgctgaatg
     8821 gtattcaccc gtgggcggaa ctgataaacc tgatgcgcgg gcttgtcgaa ccgcagcagg
     8881 agcgtgcctc atgagcgaaa cttttttcca tctgctgggg ccaggaacgc aaccgaacga
     8941 tgacagtttc agcatgaatc cactgccgat cacctgtcag gtgaatgatg aaccgagtat
     9001 ggcggccctg gagcaatgtg ctcacagccc gcaggtgatt gcgctgttaa acgagttaca
     9061 acatcaacta agcgaacgcc aaccgccgtt gggcgaggtg ctggcagtcg atctgttaaa
     9121 tctcaacgcc gacgatcgtc actttatcaa tacgcttctc ggggaagggg aagtgtcagt
     9181 gcgcattcag caggctgacg acagtgaaag tgaaatacag gaggcgatct tctgcggatt
     9241 atggcgggtg cgcagacgtc gcggcgaaaa gttgctggag gacaaactgg aggctggctg
     9301 cgcgccgctg gcgttgtggc aggcggcaac gcaaaatctc ttgccgacag attcgctgtt
     9361 accgccgccc attgatggcc tgatgaatgg cctaccgttg gcgcatgagt tactggcaca
     9421 tgtacgtaac cccgacgcgc agccgcacag cattaatctg acgcaattac ccatcagcga
     9481 ggctgatcgg ctttttctct cacgtctctg tgggccggga aatattcaga ttcgtaccat
     9541 tggctatggc gagagctata tcaacgccac ggggttacgc catgtctggc atttacgctg
     9601 tacggacacc ttaaaaggcc cgttactgga aagttatgaa atctgcccaa taccggaagt
     9661 ggtgctggca gcgccagaag atttggtcga ctctgcgcag cggcttagcg aggtatgtca
     9721 gtggctggcg gaagctgcac cgacgtaaaa agacggtaag tatcgctttc agtcttatga
     9781 atatcgcaat cggcgaatac ctctggtcgt agagtttcag gataaagagg gagatctacc
     9841 attatcgggt tatttttctc tcttcgccta caggagtgcg catgtgggat gtcattgatt
     9901 tatcgcgctg gcagtttgct ctgaccgcgc tgtatcactt tttatttgta ccccttaccc
     9961 tggggctgat ttttttgctg gctatcatgg aaaccattta cgtggtcacc ggcaaaacaa
    10021 tctaccgcga tatgacgcgc ttctggggta agctcttcgg tatcaatttt gctcttggcg
    10081 tggctaccgg cctgaccatg gagtttcagt ttggtactaa ctggtcattc tattccaact
    10141 atgtgggcga tatttttggc gcaccgctgg cgatggaagc attaatggcc ttcttcctcg
    10201 aatccacctt tgtcgggctg ttcttcttcg gctggcaacg gctgaataaa taccagcacc
    10261 tgctggtgac gtggctggtg gcgttcggtt caaatctctc tgcgttgtgg atattgaatg
    10321 ccaacggttg gatgcaatac ccgaccggtg cgcattttga tatcgacacc ctgcgtatgg
    10381 agatgaccag cttcagcgag ctggtcttta atccggtcag ccaggtgaaa tttgtgcaca
    10441 ccgtaatggc gggctacgtg accggggcca tgtttattat ggcgatcagc gcctggtatt
    10501 tactgcgcgg acgggagcgc gatgtcgcat tacgctcgtt tgccatcggt tccgtcttcg
    10561 gtactctggc gattatcggt accctgcaac tcggagacag ttctgcgtat gaagtcgcgc
    10621 aagtacaacc ggtaaaactg gcggcgatgg aaggggagtg gcaaacggaa cctgcacctg
    10681 caccgttcca tgtggttgcc tggccggaac aggatcaaga gcgtaacgcc tttgccctca
    10741 aaattcccgc gctgctaggg atcctcgcca ctcactcatt agataaaccc gtgccgggtc
    10801 tgaagaattt gatggctgaa acctacccac gcttgcaacg cggacgtatg gcctggctgt
    10861 taatgcagga aatatcgcaa ggcaatcgtg agccgcatgt gttgcaggca ttccggggac
    10921 tggaaggtga cctgggctac ggcatgttgc tctcccgcta tgcgccggat atgaatcatg
    10981 tcacagccgc acagtaccag gcggcgatgc gtggcgcgat acctcaggtt gcgccggtat
    11041 tctggagttt ccgcatcatg gtgggctgtg gttccctgct gctactggtg atgctgattg
    11101 cgcttgtcca gacgctgcgt ggcaaaatcg accagcatcg ctgggtgctg aaaatggcgc
    11161 tctggagttt gccgttgccg tggattgcga ttgaagccgg gtggtttatg accgagtttg
    11221 gtcgtcagcc gtgggcgata caggacatct taccgacata ctccgcgcac tccgctttaa
    11281 ccacaggaca actggctttc tcactgatca tgatcgtagg gctttacacc ctgttcttaa
    11341 tcgccgaagt ctacctgatg cagaaatatg cccgtctggg gccgagcgcg atgcagagtg
    11401 aacaaccgac gcagcaacag gggtaaagga gaaaatcatg tttgattatg aaacattgcg
    11461 cttcatctgg tggctgctga ttggcgtgat cctggtggtc tttatgatct ccgacggatt
    11521 tgacatgggg atcggctgtc tgctgccgct ggtggcgcgt aatgatgatg aacgccggat
    11581 agtgataaac agcgttggtg cacactggga aggcaaccag gtctggttga tcctcgctgg
    11641 tggggcatta tttgccgcct ggcccagagt gtatgcagcg gcgttttccg gcttttatgt
    11701 ggcgatgatc ctggtgctgt gctcactgtt cttccgcccg ctggcctttg attatcgcgg
    11761 aaaaatcgcc gatgcacgct ggcgtaaaat gtgggacgcc ggtctggtca tcggcagtct
    11821 ggtgccgccg gtagtcttcg gtatcgcctt cggcaacttg ttgctcggcg tgccgtttgc
    11881 cttcacaccg caattacgcg tggagtatct cggcagcttc tggcaactgc tgacgccatt
    11941 ccctttattg tgtggtttgc ttagccttgg gatggtgatt ttgcaaggtg gcgtctggtt
    12001 acaactgaaa actgttggtg tgattcatct gcgttcacag ctggcgacca aacgcgctgc
    12061 actgttggtg atgctgtgct ttttgctggc gggttactgg ctgtgggtcg gtattgatgg
    12121 ctttgtactg ctcgcccagg atgctaacgg tccttccaat ccgttaatga aactggtggc
    12181 agtgctacct ggtgcctgga tgaataattt tgtcgagtcg cccgttttgt ggatcttccc
    12241 gctgctggga ttcttctgcc cattgctgac ggtgatggcg atttatcgtg gtcgcccggg
    12301 ttggggattt ttgatggcat cattgatgca atttggcgtg attttcacgg caggcatcac
    12361 gctgttcccc tttgtcatgc cgtcaagcgt gagtccgatc tccagcctga cgttgtggga
    12421 cagtacttcc agtcagctga cgctgagcat tatgttggta atcgtgctga tatttttgcc
    12481 cattgtgttg ctctacactc tctggagcta ctacaaaatg tgggggcgca tgacaacaga
    12541 aactctccgc cgtaacgaaa acgagttgta ctaaggagca gaaacaatgt ggtatttact
    12601 ttggttcgtc ggcattttgt tgatgtgttc gctctccacc cttgtgttgg tatggctgga
    12661 cccgcgtctg aaaagttaac gaacgtaggc ctgatgcggc gcattagcat cgcatcaggc
    12721 aatcaataat gtcagatatg aaaagcggaa acatatcgat gaaagcgatc ttaatcccat
    12781 ttttatctct tctgattccg ttaaccccgc aatctgcatt cgctcagagt gagccggagc
    12841 tgaagctgga aagtgtggtg attgtcagtc gtcatggtgt gcgtgctcca accaaggcca
    12901 cgcaactgat gcaggatgtc accccagacg catggccaac ctggccggta aaactgggtt
    12961 ggctgacacc gcgcggtggt gagctaatcg cctatctcgg acattaccaa cgccagcgtc
    13021 tggtagccga cggattgctg gcgaaaaagg gctgcccgca gtctggtcag gtcgcgatta
    13081 ttgctgatgt cgacgagcgt acccgtaaaa caggcgaagc cttcgccgcc gggctggcac
    13141 ctgactgtgc aataaccgta catacccagg cagatacgtc cagtcccgat ccgttattta
    13201 atcctctaaa aactggcgtt tgccaactgg ataacgcgaa cgtgactgac gcgatcctca
    13261 gcagggcagg agggtcaatt gctgacttta ccgggcatcg gcaaacggcg tttcgcgaac
    13321 tggaacgggt gcttaatttt ccgcaatcaa acttgtgcct taaacgtgag aaacaggacg
    13381 aaagctgttc attaacgcag gcattaccat cggaactcaa ggtgagcgcc gacaatgtct
    13441 cattaaccgg tgcggtaagc ctcgcatcaa tgctgacgga gatatttctc ctgcaacaag
    13501 cacagggaat gccggagccg gggtggggaa ggatcaccga ttcacaccag tggaacacct
    13561 tgctaagttt gcataacgcg caattttatt tgctacaacg cacgccagag gttgcccgca
    13621 gccgcgccac cccgttatta gatttgatca agacagcgtt gacgccccat ccaccgcaaa
    13681 aacaggcgta tggtgtgaca ttacccactt cagtgctgtt tatcgccgga cacgatacta
    13741 atctggcaaa tctcggcggc gcactggagc tcaactggac gcttcccggt cagccggata
    13801 acacgccgcc aggtggtgaa ctggtgtttg aacgctggcg tcggctaagc gataacagcc
    13861 agtggattca ggtttcgctg gtcttccaga ctttacagca gatgcgtgat aaaacgccgc
    13921 tgtcattaaa tacgccgccc ggagaggtga aactgaccct ggcaggatgt gaagagcgaa
    13981 atgcgcaggg catgtgttcg ttggcaggtt ttacgcaaat cgtgaatgaa gcacgcatac
    14041 cggcgtgcag tttgtaatgc ataaaaaaga gcattcagtt acctgaatgc tctgaggctg
    14101 atgacaaacg aagaactgtc taatgcgtag accggaaaag gcgttcacgc cgcatccggc
    14161 cactttcagt tttactcttt ctcggagtaa ctataaccgt aatagttata gccgtaactg
    14221 taagcggtgc tggcgcgttt aatcacacca ttgaggatag cgcctttaat attgacgcct
    14281 gcctgttcca gacgctgcat tgacaaactc acctctttgg cggtgttcaa gccaaaacgc
    14341 gcaaccagca ggctggtgcc aacagaacgc cccacgaccg cggcatcact caccgccagc
    14401 atcggcggcg tatcgacaat caccagatcg taatggtcgt tcgcccattc cagtaattga
    14461 cgcatccgat cgcgcatcag cagttcagac gggttaggtg gcacctgacc gcgagtaatc
    14521 acatcaaagc ctcctttgcc aaaatgctgg atcactttgt tgagctcatc tttacctgcc
    14581 agatattccg acaagccatg ttcattactc acggtaaaca ggttatgcga ataaccacgg
    14641 cgtaagtcgg catcaataaa taacactttt tgatcggact gggcgatcac cgctgccaga
    14701 gttgaactga caaacgtttt accactgtct ggcgtcgcac cggtgatcat tagaatgtta
    14761 ttctccgtct ccatcatagc gaaatgcaga ctggttcgta gcgcacgtac ggcttccaca
    14821 gcagaatccg ccgggttatc caccgccagg aaggggatat ttttagtacg atggcgctgc
    14881 tgattagaaa ataaattttt cttacgcaga cgggtgcgtt tatccagcca ctcggacatt
    14941 gggatagtgg cataaacgct gatgccgtgc tcttccagtt gttccggggc ttctatacca
    15001 cgacgcaaca tcgcacgcgc cagcacggca cccacagaaa taaacaggcc aagaataaaa
    15061 ccaagcacca cgttcaacgc ttttttcggt ttcactggct gcggctgagt gactgccggg
    15121 tcgataatcc gcacgttacc aatggcactg gatttcgaaa tactcaactc ctgctggcgg
    15181 ttaagtaatt gcagatatac cgcacggccc gcttctacgt cacgacttaa acgcaacact
    15241 tcctgttggg tggaaggcat tgccgatacc cgcttattca ggcgtttgcg ttcttgctcc
    15301 agcgtctggc gtttttccag cagcgcacga taagttgggt gatctttctt atacagctgg
    15361 gagatctctg cctcgcggaa ggtcagctca ttgagttgat tatcaacgtt cacaatctgc
    15421 tcaagaacgg ctttggcttc caggttaagg tcaaccgaat cgcgctgctg gcgataaacg
    15481 ttgagttttt cttccgcttg gtccagctcg ctgcgcactt caggtaactg gcgctgtaag
    15541 aattcaaggc tttgtgaatc ctgcgccgcc tggcgagcga tattctgttg caaatagttg
    15601 ttagcgatgc tgttcagaat acgagtaatc aactggggat catcaccagt catggtaagt
    15661 tccagcatcc cgctttcttt actgcgttcg ctaacggtaa aggtttcctg caatgcgtta
    15721 atcgcttcca gttcggtacg ctggctcagg acaaactgtg ttcctggttt ggccttaatg
    15781 tccgcgatag tcagcgcaac gccatctttt tccagacgct ggccgaccat accattgacg
    15841 gtgaactctt caccttccag tgtatagtgg ccgttttccc caaccgtgag tgtcagttgc
    15901 tgatcctgac cattcagttg tggaatatgc atccagctga tcgccagctc acctggtttt
    15961 tctttggtta atctcgccca gccgcgaccc acaatcggaa aatacttctg ctcaactatg
    16021 tcgcgcagat tcagttcagc aatggtttta ccgagaatca tgcgcgattg cagcagttgg
    16081 atctccggtg cagactcggg cgatgagtta gggatcatat cgctcaggcc gctgagaatg
    16141 gcgttgccct gtttttgctc aacctggacc agagtatctg cctgataaat tggtgtgctt
    16201 aacagcgagt aagcgacagc gatcagcgtg aataacgcgg tcacgctgat aataaactta
    16261 cggtgatccc ataactcgcc gaccagacga agcagatcga tctcattttc ctgagtgctg
    16321 cctggtggcg tattcatatt tttagttgtc atcgtatttt ataaactcaa attaccggct
    16381 gaggcgcttc gcccattcct gactggcgcg ctccaacata ccgtagacat gttcaaatgc
    16441 gtcctgactt ttacgatagg gatccgggat ctctttctgt tccagccatt gcccaaacag
    16501 cattgtcttc ccgcggacct cgggagcaat tgcggtaacc tgagcaatat gttccgactc
    16561 cattgccaga atcagatcgt aatttcgtgc catctcagca gtgagcttac gtccggcatg
    16621 tccttcaaga gatacgccat gattagcggc aacatctgcc gctgtcgcat ctgcagggtg
    16681 ttttaccaga ccatgaacac ctgccgattt gacttttacg cccggcagac gtttacgcag
    16741 caagcgctcg ccaatgggcg aacggcaaat gttgccggta caaaccacca ggattgagtt
    16801 aaattttagt tgggccatgt ttgaatccac cgtgaagtct ctgtcaggtc atgcaccccg
    16861 gaaatcgtcg gtaccagcag agaaatcaca cggttccaac gagccagcgg cgcggtagtg
    16921 acataaacaa tgtcgtaagg ttcaagctgg aattccgttc cgagtatcat tgccgatgcg
    16981 tccttcgcat tcagctgata aatattggcg attttgccat tctgcttgtt ttgcgttgca
    17041 cgaataacaa aaatccccgt cgcatcagcg acatcctgat tcataccctc agcgttaccc
    17101 agcgcttccg ccagggtcat accgctgcgg tccattttca gcgtgctctg tttacccact
    17161 tcacccatca cgaagacttt cagatcatcg ttgcgcggaa taaataaaat atcgcccggg
    17221 tgcagcagtt tgttctggcg caagtcgcca cgctgcatca gcgcatagag gttcactttg
    17281 gtctttacgc cattctgggt gagcaccacg ttgcgccagt ctgcatctgc ggtcagaccg
    17341 cccgcggcgt taatcgcatc catgatggtc agaggaatat tggtaatcgg ctgctgaccc
    17401 gatttgctga cttcgccggt gacgtacgct ttttgcgatc ggaacgccgc gacgctgaca
    17461 tcgacttgcg ggctttcaat gactgaatcg agacgcgctg taatctcgtt gcgtacctgg
    17521 gtaagtgttt tcccggccac tttcaggcga ccaatatagg ggtagaaaat cgcaccatcg
    17581 gcgttaaccc agttgccggt atcactggcg ctacggtatt gccctgcggg ggtggttagt
    17641 tccgggtgat cccagaccgt aaccatcaac acatcaccaa tcccgatacg atattcatag
    17701 tttgcgattt gctgctccag ctccgggtta gcctgcgaca taatcggttt ggcgcgcaac
    17761 tgatcaatca atcctggcgt gacgggatag atattgacca ttttgtccag gtcatactga
    17821 ttgtcaggta gttcaatgac atccttgtta ctggtcgata aattctgacc aggaacaagg
    17881 gtacatgctg tcaaagacag aacagccaat gtcagtaccg aaaacttaaa aatgttcttt
    17941 ttcattggtt tatatttatc agtcaatgta atgttatgaa ttacgtcagg ttattgtcca
    18001 accggtactt ctcgctctgc cgtcattgga tagagctggt atttgcgtga cagcatttgt
    18061 ccaccatcac gcgtcaatgg tgtccacgaa accaccgcgc ggttgcggtt aggtccaatg
    18121 gtcatcaagt cgaatggaat cgagatataa aaacctttac taaagccgcc ttcgccgtag
    18181 tcatctttag atacgttact gattgccgcc cataccccta ccgccacgcc gctgtcaaag
    18241 cgtttggcga cgtcgatcgt tgcgccttta tcttttgcca gatattgccc aacgctaagt
    18301 ttcatcagta cgccattgag cgtcggcggg ttccagtaag ccgtcacgaa acctgttggc
    18361 gtggaataat cggtgaaacg catcatgttg tcccagtcac gttgcttcac gtagttaacg
    18421 tccacaccca gcgcccagca ggcatctagc gggcgataaa gcagctcgga accgacaccg
    18481 gcgtacatcg tttccagata accgccatac acctggccat agaaaccatt gcctaagtca
    18541 gcaaaatagt tcgcctgcaa gttgttgaga taaacgtcat tgcgaacgta gtcacggata
    18601 tgcgtgcgca cgcggggcag ggtagagtcc gcgggcaaca gcgaagactt aaacttgtcg
    18661 tagttgttgt aaatattggt gaaaataccg ccatcaagca gcaggtggtc ggtaaaccag
    18721 tagcgggcac tggacatcag ccccagctgg aacatataga aatcttccgg gccgcccagc
    18781 gactgtgaaa gtgttgggtt gaaagagtag ctaaagcgat cttcacgaat acggtagccc
    18841 cgaccaaagg cagaaagatc ttctgcttca acacgttgtt gttgaagttg ctctgattga
    18901 ccaggcgctg taccagccag ctgtttgcgc aggctggcta cgtcggtttc ggtagtcacc
    18961 atcgccatat gctcgcgctt ttgcgtcacg ctaatcttct caacgccttg cggcaggtta
    19021 ttcaccagaa tccggttggc acgatcgacc gcttcgcgag aatcacggta tttgtattgt
    19081 tgaccagaca tatacagcgt cttatcgcgc agctgaattt ccggtgcttc aaaacctgcg
    19141 ttgtacttca gcgcggtaag ttggttcgct actgtggtgt actgcaatcc ttcagattca
    19201 ggcgcaggtt gatatgccgg ttttggcgta tcgcgcaggg cagggcgcag atcgttgaaa
    19261 ttggtccgta acgtgaagcc aaacatcaac gtgttaccgc gttcataact caggttgaga
    19321 tctgcccagc tggcagcgcg ataaactgcg ccgacgttga aatggcttgc ctgaggcagt
    19381 ttgccagcga aatcattctg gtaattgttg ccatcgtatt cgagtttcag acgcagggga
    19441 ttccacggcg tttgatactc aatgccgcca aagatggaag ccgggccacg aaagatatcg
    19501 ctaaagctga tatcgcccgc atcgtgagac tctgcgcgat gacaatattt atcgctcacc
    19561 cggcaaaacg ggttggtaat attgcccgca ttaccggcgt agccccatgc catcccgagg
    19621 gtgaaatcaa atggccccgc ttgcttgctg gccaccagat actcaccgtc aaacagaccc
    19681 gtaccagcaa tatcacgttt accaaacgcc acttgcggta gccagtaacc ttcttcccac
    19741 aggcgaagtt taaaatcgaa tgatttgtct ttgtatgact gatcgccgct gaaatcttcc
    19801 cactggctat atttgcgtgt gcgcacatcc gtataacgaa tggtgccttc cagccatggg
    19861 aacagcgcca cggaggtgga gtagaaccgg tattgatcgt tatcccggta gttgacgctg
    19921 aattcacctt ccggtgcgat gcgcgcattt ggcatctgca acaatcctgt gccgccaaag
    19981 tctgattgcg acgaacccag cggatccggg taggttaata cttcagcatg acaggcactg
    20041 gagacggcaa tggccaggca gcttaaaaga taagaattct tcttcatcag tcaggaacac
    20101 gttgcgttaa aacagacaca atctggtcat ttaaatcagc atatttttcg ggcagtacat
    20161 gcgcagaaaa gcccaaccaa agctggctgc caggcggtgg ttccacatgt cttttattcc
    20221 acagggcaac aggggcgacg acagtctcac cctcaggcgt aatcaccata acgttattct
    20281 tgtccgcgcc tgcaaggcgg gggtgatctt gcagataatc ggtaacgcta cggcctgcct
    20341 gccagggcaa ttgcccggcc ccagaaaccg cacccagcag agtaatagtc accggacgct
    20401 gtacggtata gagcgtatag tccccgacca acgggggatt gctgttctca tccactcgta
    20461 caaaatcagg gtccagtttg accggtaagc gaccagtgat attgagattg agtaactgct
    20521 ggcgcacgga tttaatcgtt gccgccacat cgtcgtcagc ttctgcttcc caggaagcaa
    20581 gctgtgccat gacatgttgg tagtctttaa gcgctttagc tttcgccgca gagtcggtca
    20641 gcaaggcacc aggccaccag aggcgatcgc gcagttgcgg ttgtgtcacc aactggacaa
    20701 cattttccat ggggccaacg gaaagtgtct gttgttcgcc aggcagataa atagtcaccg
    20761 ttccttgcgc aaaggcatgg ggtgtcatta cgtaaagtac gctggcaatg aaatacgact
    20821 gtaatttatt catggtgcgg gtttaagaaa cgtcatttct acgggaataa cccctgcgcc
    20881 taacatttgc cgactttgac gaacctggcc agtagccgaa tcgacccaga atgtgttttg
    20941 ccactgtttt tctggccgcg tggaagtcac ctcttcctgc cagacattac acagcacatt
    21001 gcgacccgca atattcagcg tctctaatcc agcaaaggaa aaagtagaac tcagggtcgc
    21061 agagcgaaaa tggttgtctt cactccagag aatatcgcgc gtccagcggc tgccatcttt
    21121 tattgccagc gcgttacgca gagggtcctg cccggaattt gtcacttcca gcagattatt
    21181 attaagtttg acggttttta aaagacgtcc attgtgggta accagcatgg cgttatcctg
    21241 ggataaccat ttgctttgtt cttgttctat atatcccagt accacaaaga ttcgttgccc
    21301 ttcattaagg cgtaaataca tagtggaata aggcaacgcc tggatctgct gatccgctac
    21361 ggtgatatcc tgattatcga aaaggctggc gcgaaatgta tcgaccatac tttgctggct
    21421 gtgcgtacat cccgcaagaa atagtgcgaa aatcgataaa ataagagggc gcacctttaa
    21481 tccttgaaag gttaattaaa aaaatgcaaa tctctaaagc ccggtacgaa tatcgcaacc
    21541 gggtatcatt taactttaat ttcgacgcgt taaagcagta ttagcgtcta gtggacgtgg
    21601 tggttgaggt gttatgagaa ccgccatcgc cactgtcatt tgctgcaaca actgccaacg
    21661 tcgccacacc aacagccgtt gccacaccga caactgtcgc cgtgccgaca gcgccagcag
    21721 ccacgccacc agtggtagta gcagtcttgg ttgcgccggt agtggtcgtt gtggtgccag
    21781 tagtacccgt cgttgcctcg gtcccgtttg ttgcttcagg ggcagcaaac gcagctggcg
    21841 tcgtcaacat gaaggccata aggattgcag aaagcttgtg tttcataact tttcctttat
    21901 tcatcgcatg gacaatacgt acgcttgaga tcaggattgt ctactttgag tataacatcc
    21961 ttgtgaaatc tccctttcct cataccgctt gcactatata ctgcgggtag gaaaggcgcg
    22021 cagagggaaa tataagattg ttaactatcc ccctct
//
LOCUS       DAPHBJ010000065        21943 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.065, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000065 DAPHBJ010000000
VERSION     DAPHBJ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.065"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            74..616
                     /gene="zapC"
                     /locus_tag="RHZ76_002178"
     CDS             74..616
                     /gene="zapC"
                     /locus_tag="RHZ76_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HDV2812819.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSFEQAV"
     gene            complement(613..1722)
                     /gene="ycbX"
                     /locus_tag="RHZ76_002179"
     CDS             complement(613..1722)
                     /gene="ycbX"
                     /locus_tag="RHZ76_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HDV2812820.1"
                     /translation="MATLIRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            1966..4074
                     /gene="rlmKL"
                     /locus_tag="RHZ76_002180"
     CDS             1966..4074
                     /gene="rlmKL"
                     /locus_tag="RHZ76_002180"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HDV2812821.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYTNRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            4086..5993
                     /locus_tag="RHZ76_002181"
     CDS             4086..5993
                     /locus_tag="RHZ76_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2812822.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            6123..7376
                     /gene="pqiA"
                     /locus_tag="RHZ76_002182"
     CDS             6123..7376
                     /gene="pqiA"
                     /locus_tag="RHZ76_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HDV2812823.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            7381..9021
                     /gene="pqiB"
                     /locus_tag="RHZ76_002183"
     CDS             7381..9021
                     /gene="pqiB"
                     /locus_tag="RHZ76_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HDV2812824.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            9018..9581
                     /gene="pqiC"
                     /locus_tag="RHZ76_002184"
     CDS             9018..9581
                     /gene="pqiC"
                     /locus_tag="RHZ76_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HDV2812825.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            9837..10004
                     /gene="rmf"
                     /locus_tag="RHZ76_002185"
     CDS             9837..10004
                     /gene="rmf"
                     /locus_tag="RHZ76_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HDV2812826.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(10074..10592)
                     /gene="fabA"
                     /locus_tag="RHZ76_002186"
     CDS             complement(10074..10592)
                     /gene="fabA"
                     /locus_tag="RHZ76_002186"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HDV2812827.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(10661..12421)
                     /locus_tag="RHZ76_002187"
     CDS             complement(10661..12421)
                     /locus_tag="RHZ76_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HDV2812828.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPILIREAA
                     RYTGEQETLPLSPQWLLRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            12607..13059
                     /gene="matP"
                     /locus_tag="RHZ76_002188"
     CDS             12607..13059
                     /gene="matP"
                     /locus_tag="RHZ76_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HDV2812829.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(13135..14175)
                     /gene="ompA"
                     /locus_tag="RHZ76_002189"
     CDS             complement(13135..14175)
                     /gene="ompA"
                     /locus_tag="RHZ76_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HDV2812830.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(14532..15041)
                     /gene="sulA"
                     /locus_tag="RHZ76_002190"
     CDS             complement(14532..15041)
                     /gene="sulA"
                     /locus_tag="RHZ76_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HDV2812831.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            15259..15888
                     /gene="sxy"
                     /locus_tag="RHZ76_002191"
     CDS             15259..15888
                     /gene="sxy"
                     /locus_tag="RHZ76_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HDV2812832.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(15851..18013)
                     /gene="yccS"
                     /locus_tag="RHZ76_002192"
     CDS             complement(15851..18013)
                     /gene="yccS"
                     /locus_tag="RHZ76_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDV2812833.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDQSQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(18023..18469)
                     /locus_tag="RHZ76_002193"
     CDS             complement(18023..18469)
                     /locus_tag="RHZ76_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDV2812834.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            18592..20646
                     /gene="helD"
                     /locus_tag="RHZ76_002194"
     CDS             18592..20646
                     /gene="helD"
                     /locus_tag="RHZ76_002194"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HDV2812835.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSLFVEILKNLDVPVAR
                     KP"
     gene            complement(20678..21136)
                     /gene="mgsA"
                     /locus_tag="RHZ76_002195"
     CDS             complement(20678..21136)
                     /gene="mgsA"
                     /locus_tag="RHZ76_002195"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDV2812836.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(21232..21894)
                     /locus_tag="RHZ76_002196"
     CDS             complement(21232..21894)
                     /locus_tag="RHZ76_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HDV2812837.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPHLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
BASE COUNT         5572 a         5314 c         5844 g         5213 t
ORIGIN      
        1 ttattgttga ggttattaag cgaagcgaca atggattgtg ttgctgcggt tttatagggt
       61 aggggagagg cagatgcgaa ttaaaccaga cgataactgg cgttggtatt atgatgaaga
      121 gcacgatcga atgatgctcg atttagccaa tggtatgcta tttcgctcac gttttgcgcg
      181 caagatgttg actccagatg ccttttctcc cgcaggcttt tgcgttgacg acgccgcgct
      241 ctatttctct tttgaagaaa agtgccgcga ttttaattta tctaaagaac aaaaagccga
      301 actggtgctg aatgcactgg tagcaattcg ctatctcaaa ccgcaaatgc cgaaaagctg
      361 gcattttgtt tcccatggtg aaatgtgggt gcccatgccg ggcgatgcgg cctgcgtttg
      421 gttgagcgat actcacgagc aggtcaattt gctggtcgtt gaatctggcg aaaatgccgc
      481 actatgcctg ctggcacaac cctgcgttgt tattgcgggt cgcgcgatgc agttgggcga
      541 cgccattaaa atcatgaacg acaggctgaa accgcaggtt aatgttgaca gcttcagctt
      601 cgaacaggca gtctaacgcg ccaacttaag tgcagtcttc ggtacacagc tacagcaaag
      661 aatggtgcca tcatcgccca ttgctgattt tttcagcggc gtgacttcgc cttctaaaag
      721 ctgaacacgg caacttccac aaatgcccgc gcggcaagaa taagggatac gaattccctg
      781 attttctaat tgctccagca acacctgttg gttatttcca cgaaatgcct gtccctgcca
      841 gtcaatatct acatttgcgt ccggttgttg cgtgatgttg gcggtatcat cagcggcagc
      901 tgcgccgtaa atttttgccg gagccgttgc cagaatttcc acctcatcgc caacgcgaat
      961 cacgccgcta ttacgggcaa ttaaattctg accaaaatcg acatcgccgt tatcctgggc
     1021 agtgcggaaa gattgcaatg tttttaatgg ttcgcctgcc ggatgttttt gccctttttc
     1081 tgggctgacg gtggtgaaaa tacagcggct acaaggttta accacatcaa acaccacatc
     1141 accaatgcga atcactttcc agcgatcttc ttcccaggct gacgcgccgg aaaccaccag
     1201 attggggcgg aattgctcca tttttacact ggccggacaa cgttgttgga gatcacgtaa
     1261 cgaggcttcg ttagcaagaa ggtaaggata gccatcagca aatgacagag gtacagtgtt
     1321 gtggcgtttc acgcgccggg tcatttgtgg ccccacccag cgtaattgca cttcgcggga
     1381 gaaaaatcca cttagccatt tgttgatcgc gtctggcgca attcgcgcgg taaaatgtgt
     1441 gccccaaact tcggttggtg cgtcttgtgt ggcgaaatca gcaaaacgaa catatgcgct
     1501 actgccatct ggtgcggtga gatgcaagcc atcatgcaca ggtgaaggag taaaccgtac
     1561 catctgggga aactggcgag cggtaataaa cgtaccgtca ggttccgtga tcataaagat
     1621 gcgatcgaag gccagaccac tgacatctgc cagagcatgt gtaagaccaa tgccgcgcat
     1681 cgatttaaca ggatgaataa aaagccggat taatgtcgcc acggtgcggt cctcaaatga
     1741 aaataagccc tcaactttat gacatgacgc gcttattagc tataatgcgc aacaattttc
     1801 ttagcgcctg tcccattagg ctattttatt tgtcagtttg gccctgggtt atgctcgaaa
     1861 tcctcacgta cttgtgtacg ctccggtttc tccgcgctgt ccatgtccag actgactgca
     1921 acaattacgc ctactgcggt aggctcttag agtaaaagtg acgatatgaa ttctctgttt
     1981 gccagtacgg cccgtgggct ggaagagctg ttaaaaactg aactggaaaa cctgggggcc
     2041 gttgaatgcc aggtggttca gggtggggtc catttcaagg gcgacacacg gcttgtttac
     2101 cagagcctga tgtggagccg cctggcctcg cgtattatgt tgccgctggg cgagtgtaag
     2161 gtttacagcg atttagacct ctatctcggt gttcaggcga tcaactggac agagatgttt
     2221 aatcctggcg cgaccttcgc tgtccacttc agtggtttga atgacaccat ccgcaacagt
     2281 cagtacggcg cgatgaaagt gaaagacgcg atcgtcgatg ctttcacgcg gaaaaatctg
     2341 ccgcgtccaa atgttgatcg cgatgcgccg gatatccgcg ttaacgtctg gctgcataaa
     2401 gaaaccgcca gtatcgctct tgatctgagt ggtgatggtt tacatctgcg tggctatcgc
     2461 gatcgtgctg gtattgcgcc gatcaaagaa accctggcag ccgctattgt gatgcgatcc
     2521 ggctggcagc caggaacacc gctgctcgat ccgatgtgtg gttccggtac gttgctgatt
     2581 gaagcagcga tgctggcgac cgatcgcgca ccaggcttgc accgtgggcg ttggggcttt
     2641 agcggctggg cgcagcatga tgaagctatc tggcaggaag tgaaagcgga agcgcaaact
     2701 cgcgcccgta aaggcctggc tgagtatagc tctcattttt acggttcgga cagcgacgca
     2761 cgggtgattc aacgtgcacg cactaacgcc cgtcttgcgg ggattggtga actgatcacc
     2821 tttgaggtga aagatgtcgc gcaactgacc aatccgctgc cgaaagggcc gtacggcaca
     2881 gtgttgagca acccgccata cggggaacgt ctggacagcg aaccggcgct gattgcgctg
     2941 catagcctgc tgggtcggat catgaaaaac cagtttggtg gctggaatct ctctttgttt
     3001 agtgcctcgc cggatctgct aagctgcttg cagctgcgtg cagacaaaca gtacaaggcg
     3061 aaaaacggcc cgctggactg cgtacagaaa aattaccacg ttgccgaaag cactccagac
     3121 agcaaaccgg cgatggtagc ggaagactac accaaccgcc tgcgtaagaa cctcaaaaaa
     3181 ttcgagaagt gggctcgcca ggaagggatt gaatgttacc gcctgtatga cgccgatctg
     3241 ccagaatata acgttgccgt tgaccgttat gccgactggg tggtggtgca ggagtatgcg
     3301 ccgccaaaaa ctattgatgc tcacaaagcg cgtcagcgtc tgttcgatat tatcgctgca
     3361 accatttcgg tactggggat tgcgccaaac aaactggtgc tgaaaacccg tgaacgccag
     3421 aagggcaaaa accaatacca gaaactgggc gagaagggcg agtttcttga agttaccgaa
     3481 tataacgctc acttgtgggt gaacctgacg gattatctcg atactggtct gttcctcgat
     3541 caccgcatcg cccgtcgtat gctcggtcag atgagcaaag gcaaagattt cctcaacctg
     3601 ttctcttata ccggcagcgc caccgtgcat gcgggattag gcggtgcacg cagcaccacc
     3661 accgtggata tgtcgcgtac ttatctggaa tgggcagaac gcaacctgcg tctgaatggc
     3721 ctgaccgggc gtgcgcatcg cctgattcag gccgattgcc tggcgtggct gcgtgaggca
     3781 aatgaacagt tcgatctgat ctttatcgat ccgccaacct tctctaactc aaaacgaatg
     3841 gaagatgcgt ttgatgttca gcgcgatcat ctggcgctga tgaaagattt gaaacgtctg
     3901 ctgcgtgcag gtgggacgat catgttctcg aacaacaaac gtggcttccg tatggatctc
     3961 gacggcctgg caaaactggg actgaaagca caagaaatta cgcaaaaaac gctctcccag
     4021 gatttcgccc gtaaccgcca gatccacaac tgctggctga ttaccgcagc ctgaaaggaa
     4081 tagtaatgtc attaatcagt atgcatggcg catggctgtc gttcagcgac gcgccgcttc
     4141 tcgataacgc agaactgcat atcgaagata acgaacgtgt ttgtctggtg ggccgcaacg
     4201 gcgcaggcaa atcgacgtta atgaaaatcc tcaaccgtga acaagggctg gatgacggtc
     4261 gcattattta cgagcaagat ttgattgtag cgcgtctgca acaggatccg ccgcgtaacg
     4321 ttgagggtag cgtttatgat ttcgttgccg aaggcattga agaacaagcg gaatatctga
     4381 aacgctatca cgatatttcg cgcctggtga tgaacgaccc gagcgagaaa aatctcaacg
     4441 aactggcgaa ggttcaggaa cagctggatc accacaacct gtggcagctg gaaaaccgca
     4501 tcaacgaagt gctggcgcaa ctggggttag atcctaacgt tgcgctgtcg tcgctttccg
     4561 gcggctggtt gcgtaaagcg gcattaggac gcgcgctggt gagtaatccg cgcgtgctgt
     4621 tgcttgatga accgacaaac cacctggata ttgaaaccat cgactggctg gaagggtttt
     4681 tgaaaacttt caacgggacg attattttca tctcccacga ccgttcgttt atccgcaata
     4741 tggcgacgcg cattgttgat ctcgatcgcg gcaagctggt gacctatcca gggaattacg
     4801 accagtacct gctggaaaaa gaagaagccc tgcgcgtgga agaattacaa aatgccgagt
     4861 tcgatcgcaa actggcgcag gaagaggtgt ggatccgcca ggggatcaaa gcacgccgta
     4921 cccgtaatga aggccgcgta cgcgccctga aagcgatgcg tcgcgaacgt ggtgaacgtc
     4981 gcgaagtgat gggtaccgca aagatgcagg tggaagaggc cagccgctcc ggtaaaatcg
     5041 ttttcgaaat ggaagacgtt tgctaccagg ttaacggtaa gcaactggtg aaagattttt
     5101 ctgcccaggt tctacgtggc gacaaaattg ccctgattgg tccgaatggg tgcggcaaaa
     5161 ccacgctgct aaaactgatg ctcggtcagc ttcaagcgga cagcgggcgt attcacgttg
     5221 gcaccaaact ggaagtggct tatttcgatc agcaccgcgc ggaactggat cccgataaaa
     5281 cggtgatgga taaccttgcc gaaggtaagc aagaggtgat ggttaacggc aagccacgcc
     5341 acgtattggg ctatttgcag gactttctgt tccatccgaa acgggcgatg acgccggtac
     5401 gtgcgctttc tggcggtgag cggaaccgct tgctgctggc gcgtttgttc ctcaaaccaa
     5461 gcaacttatt gattcttgac gaaccgacca acgatcttga tgtcgaaacg ctggaactgc
     5521 tggaagaact gatcgacagc tatcagggca cggtattgtt ggttagccac gatcgtcagt
     5581 ttgtcgataa caccgttaca gaatgttgga tcttcgaagg cggcggtaaa attggtcgtt
     5641 atgtcggcgg ttatcatgat gcccgtggtc agcaagagca gtatgtggcg ctcaaacagc
     5701 ctgcggtgaa aaaaaccgaa gaagccgccg cggcaaaagc agaaactgta aaacgcagca
     5761 gtagcaaact aagctataaa ttgcagcgcg aactggagca gctaccgcaa ttgctcgaag
     5821 atctggaggc gaagctggaa gccctacaga cgcaagtggc ggatgcttcc ttcttcagtc
     5881 agccgcatga gcagacgcaa aaagtgcttg ctgatatggc tgctgcagag caggagctgg
     5941 agcaagcctt tgaacgctgg gagtatcttg aagcgttaaa aaatggtggc tgatcgcaaa
     6001 atagtcgatt taaacagaga cagctgacaa cggtaaaatt gcctgatgaa ctgggtttat
     6061 caggcatgaa aaccaacact ttgttagtca tctgtaaaat agcgcatcat taaggagtac
     6121 caatgtgcga acatcatcat gccgcgaagc acatcctgtg ctcgcagtgt gacatgctgg
     6181 tggcgttacc gcgccttgag catggtcaga aagcggcatg tccccggtgt ggcacaacgt
     6241 taaccgtggc gtgggatgcc cctcggcagc gtcccaccgc ctatgcgttg gctgcactgt
     6301 tcatgctgtt gctgtccaac ttgtttcctt ttgtgaatat gaacgttgca ggagttacca
     6361 gtgaaattac attactggaa attcccggcg tgcttttttc tgaggactac gccagcctcg
     6421 gcaccttttt cctgttgttt gtgcaactgg ttcccgcgtt ttgtctgata accattctgt
     6481 tactggtgaa tcgcgcggaa ttaccggtcc gtttaaaaga gcaactggca cgggtgcttt
     6541 ttcaactcaa aacctgggga atggcggaga ttttcctcgc tggtgtgctg gtcagtttcg
     6601 ttaaactgat ggcttacggc agcattgggg taggcagcag ctttctcccc tggtgtttat
     6661 tttgtgtcct gcaactgcgc gcttttcagt gcgttgatcg tcgctggtta tgggacgaca
     6721 tcgccccgat gccagaactg cgccagccgc taaaaccagg cgtcacgggg atacgtcagg
     6781 ggctgcgttc gtgctcctgt tgtacggcaa tccttcctgc tgatgaaccc gtgtgcccgc
     6841 gttgtagtac caaagggtac gttcggcgta gaaacagcct gcagtggaca ctcgcgctgc
     6901 ttgtaacgtc catcatgctg tatcttccgg ctaatatttt gcccatcatg gtgacggatt
     6961 tattaggctc gaagatgccg tcgacgattc tcgctggggt cattctgtta tggagcgaag
     7021 gctcttatcc cgtcgctgcg gtgatctttc tggccagtat tatggtgcca acgttaaaga
     7081 tgatcgccat cgcgtggctg tgttgggatg ccaaagggca tggcaagcgc gacagtgaaa
     7141 gaatgcattt gatttatgaa gttgttgagt ttgtaggccg ctggtcgatg attgacgttt
     7201 tcgttatcgc ggtgctctcg gcgctggtgc gtatgggagg tttaatgagt atttatccgg
     7261 caatgggtgc attaatgttt gctttagtcg tcataatgac aatgttttct gctatgacgt
     7321 ttgacccgcg tttgtcgtgg gatcgtcaac ctgaatcaga gcatgaggag tcctgacagt
     7381 atggaatcta ataatgggga agccaaaatc cagaaagtga agaactggtc tcccgtgtgg
     7441 atatttccta tcgtcacggc gctcattggg gcctgggttc ttttttatca ttacagccat
     7501 cagggaccgg aagtgaccct gatcaccgcg aatgcggaag gaattgaagg tggcaaaacc
     7561 accattaaaa gccgtagcgt tgacgtcggc gtggttgaaa gcgccacact ggctgatgat
     7621 ttgacgcacg ttgaaatcaa agcgcggctg aattccggta tggaaaaatt gctgcataaa
     7681 gacaccgtct tttgggtggt gaaaccgcag attggtcgcg aagggattag cggcctggga
     7741 acgctgctgt ctggagttta tatcgaactg cagccaggcg cgaaaggcag caaaatggat
     7801 aaatacgatt tgctggactc gccaccgttg gccccgcctg atgcgaaagg tatccgtgtg
     7861 attctcgata gcaaaaaagc cgggcagctc tcgccaggag atccggtgct gttccgtggc
     7921 tatcgggtag gttcggttga aaccagcacc ttcgatacac aaaaacgcaa tatcagctat
     7981 caactgttca tcaatgcacc ttatgaccga ctggtgacca acaatgttcg cttctggaaa
     8041 gatagtggca ttgcggttga tctgacgtca gcagggatgc gtgtggagat gggctcattg
     8101 acaacgctgc tgagtggcgg tgtcagcttt gatgtgccgg aaggtctgga tttagggcag
     8161 ccagtggcac cgaaaacagc tttcgttttg tatgatgatc agaagagcat tcaggattcg
     8221 ttgtacaccg atcacattga ttatctgatg ttctttaaag attcggtacg cggtctgcaa
     8281 ccgggagctc cggtagagtt ccggggtatt cgcctgggta ccgtaagcaa agtgccattc
     8341 tttgcgccga atatgcgtca gacatttaac gatgattacc gtattccggt actgattcgt
     8401 atcgagccag agcggctgaa aatgcagctt ggcgaaaatg cggatgttgt tgagcacctt
     8461 ggcgaattgt tgaaacgtgg tttacgcgga tcgctgaaaa ccggaaacct ggtcactggt
     8521 gcactgtatg ttgatctcga tttctatcca aatacgcctg caataaccgg tattcgtgaa
     8581 tttaatggtt atcagattat cccgaccgtt agcggcggcc tggcgcaaat ccagcaacga
     8641 ctgatggaag cgttggataa gatcaacaaa ctgccattga atccgatgat tgaacaggca
     8701 accagtacgc tttctgaaag tcagcgcaca atgaaaaacc tgcaaacgac gctggatagc
     8761 atgaacaaga tcctcgctag ccagtcgatg cagcagttgc cgacggatat gcagtcaacg
     8821 ttgcgtgaat tgaatcgcag catgcagggc ttccagcctg gctccgcagc ctacaacaag
     8881 atggtggcgg atatgcagcg ccttgatcag gtgttgcgag aactgcaacc ggtgctgaaa
     8941 acgctcaatg agaagagtaa cgcgctggta tttgaagcga aggacaaaaa agatccagag
     9001 ccgaagaggg cgaaacaatg aaaaagtggc tagtgacgat tgcagcactg tggctggccg
     9061 gatgcagctc cggcgaaatt aataaaaact attaccagtt acctgtggtg cagagcggta
     9121 cacaaagtac cgccagccag ggcaatcgtc tgttatgggt agagcaggtc actgttcctg
     9181 actatctggc ggggaatggt gtggtttatc aaaccagtga tgtgaagtat gtgattgcca
     9241 acaacaactt gtgggccagc ccgttggatc aacagttgcg caacaccctg gttgccaacc
     9301 tgagtacgca actgcccggc tgggtggttg cctcccagcc tctgggaagc gcccaggaca
     9361 cgctcaatgt taccgtaacg gagtttaacg gtcgctatga tggcaaggtc attgtcagtg
     9421 gtgagtggct gttgaaccac cagggacaac tgatcaaacg tccgttccgt ctggaaggag
     9481 tgcaaactca ggatggttac gatgagatgg ttaaagtgct ggccggtgtc tggagtcagg
     9541 aagccgcttc tattgcacaa gagataaagc gtctacctta attataaaga tttgtaaata
     9601 taaccgtctc cggtatgttg cctgaggcgg tttttttgtc tctaacgtgc ggaaaaattt
     9661 gttcctcttc acattttttg tacaaccgac atgcccgtgt agctcacaaa tatgacagtg
     9721 gcgtgaattt tgcgcattga cggcagttat gattcgcggt attgcttaac tgtgattgca
     9781 catttagtaa tcactgtttt cttttccacc agaaaccagt atgagggaaa cgaggcatga
     9841 agagacaaaa acgagatcgc ctggaacggg cacatcaacg tggttatcag gccggcatcg
     9901 ccggacgctc aaaagaaatg tgtccctatc agacgctgaa tcaaaggtca caatggctgg
     9961 gaggctggcg agaagccatg gcggacaggg tagtaatggc ctgattctgt ctctttaaaa
    10021 agaaacctcc gcattgcgga ggtttcgcct tttgatactc tgtctgatta taatcagaag
    10081 gcagacgtat cctggaacag accgactttc aggtcgctgg cggtatagat cagacgacca
    10141 tcaaccagca cttcgccatc cgccaggccc ataatcagac gacggttaac aatgcgttta
    10201 aagtgaatac ggtaggtcac ttttttcgct gtcggcagta cctgaccagt gaatttcact
    10261 tcgccaacgc ccagcgcgcg gcctttacct tcgccgccca gccagccgag gtagaaccct
    10321 accagctgcc acattgcgtc caggcccagg catcccggca taaccggatc gccaataaag
    10381 tggcatccga agaaccacag atccggattg atatccagtt ctgcttcaac ataccctttg
    10441 tcgaagttac cacccgtttc ggtcattttg accacacggt ccatcatcag catgttcggt
    10501 gctggcaatt gcgggccttt agcgccaaac agttcaccgc gaccagaggc aagaaggtct
    10561 tcttttgtat aggattcgcg tttatctacc atgttctctg taagccttat tttattgaag
    10621 cacgcaggat agctaacacg tgtacgctga acaagtccga tcagttcgga ataaaccagt
    10681 tcagccaacg taatggccat ggaaaacggt gacgtccttc ctgttgcgat gcttgcgcga
    10741 tacgttcctg gatggtttgc atcagcgtcg tttggccttc gccatcccac accagattta
    10801 ataataacgg cagtgcgtca gtcacatcgt ctactgccca gatggtgaat ttgccttctt
    10861 ctaccgcttt caccagttca ctgtgaagac ttaaatggcg aacgttagct gtggggataa
    10921 tgacaccttg tttcccggtt aactcacgtt gctggcaaat agcaaagaag ccttcgattt
    10981 tctcatttaa accaccgacc ggctgggcgc gaccgaactg atcgactgaa cctgtgatag
    11041 cgatactctg attcaccggc acatcggcga gggcgcttat cagggcgcag agttcagcca
    11101 tcgaggcact atctccatca acttcactgt atgactgctc aaatgtcagc gatgctgaga
    11161 aggggatctg ttgctcaagc tgtagttccg acatcaggaa cgcttgcatg atcatcatcc
    11221 ctttcgcatg gatattgccg ccaagctccg ctttgcgttc gatgtcggtg aattcaccat
    11281 cgccaatatg cacaacgcag ctaatgcgag aaggttcgcc aaaagcgcgt ggatgacccg
    11341 gaaattcaat gaccgaaagg gcgttaattt gcccgatgcg ttcgccttcg gtttcaatca
    11401 ggatttgctc ctgaaggatc tcatcctgca tacgttcagc gaggaaacct tcgcgccatt
    11461 cacgctgctg cagcattaag tttagctgct cgccggagaa ggtgtcgcca tcacacaggg
    11521 aggcgacctc tttacactgg cggaggagcc actgcgggct aagcggaagt gtttcttgtt
    11581 caccggtgta gcgtgctgct tcgcggataa gtatcggcca ggcatccgct cccggtgcag
    11641 gtaagtgatt atgtctggcg gtaaatgtca cccagcgaca ccactgggtt actgactccg
    11701 catcgacaat ctgcagagta tcttcaaatt cgctataaat agcctgctct gaaagctctg
    11761 gctccatctc ctggaaatca gccaatgatt cgcgttcgcc taccagaatg actttcagct
    11821 tcaatggcat cgaaggcaca gagacgggga gagggcgcga ctcatcaaac gcaacccagt
    11881 caaaacgctc gcggttaacg atatttttca gccgcatcca cagcagaggt tgcgccagca
    11941 gtgtacgcaa agagatgatg agaataccgc catttgcctg atgcaccaga ccaggctgca
    12001 gggtaatgtc gccattaaac tggcgcaggc agccaaagag ttgctccgct tctacccagt
    12061 cggcagcgac aacttgcgtt aaagtcgcaa aattatcatc tgcactcact gcgtgacgta
    12121 agcggatgga gtggccggaa acctcatagt gaccgcccac cagttggcct gcatcgcttt
    12181 gtagcgtacg cgcggcattg gcaataagat tcagatactc agactcttcc ggggccttcg
    12241 ccagcataaa agaggaggat gctcgcgtat gcagcaactg ctccagcgca aattgcagcc
    12301 gcggttgagt atcactgaat aaaggatcgt tttcgtcaat caaatgtggc tgagcaaata
    12361 tttcctgata gctatcggta tcaggaacca ggtcacgcca tgcaagtttc gtaatggtca
    12421 aagttgatgt tttttagtct gttgtcaaag ccgcgattat accgtaaccg gcactacagc
    12481 acacgtagaa agcaccgaca atactcctgg catgggcgtt aaagctcaca ggatggagat
    12541 tcttttcttc actggcctaa aaagctgata ttctgtaaag agttacacgg taacattgag
    12601 atcgctatga aatatcaaca acttgaaaat cttgaaagcg gttggaaatg gaagtatctg
    12661 gttaagaagc atcgcgaagg ggagttaatc acccgttaca tagaagccag tgccgcccag
    12721 gaagccgtcg atgtgttgct ctcgctggaa aatgaacccg tgctggtaaa tggctggatt
    12781 gacaagcata tgaatccgga actggtcaat cgcatgaagc aaaccattcg ggcaagacga
    12841 aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa aatcgatcga tctggaattt
    12901 attgtctggc aacgtctggc tggtcttgcg cagcgtcgcg gtaaaacgct ttctgaaacg
    12961 attgttcagc tgattgaaga tgcggaaaac aaagagaaat acgcgaataa aatgtcttct
    13021 ctgaagcagg atctgcaggc attgctgggt aaggaataac tgacgaaagt cagttcaatt
    13081 tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc taccagacga gaacttaagc
    13141 ctgcggctga gttacaacgt ctttgatacc tttaacttcg atctctacgc gacgatccgg
    13201 agccaggcag tcgatcagtg cagcacgctg tttcacgttg tcacaggtgt tgccagtaac
    13261 cgggttggat tcgcccatac cacgtgcgga gatcttgtct gccgggatac ctttggagat
    13321 caggtaatca acaacagact gagcacggcg ctcggacaaa ccctggttgt aagcgtcaga
    13381 accgatgcgg tcggtgtaac ccagaacaac tacggaaccg tctttcggat ccaggttgct
    13441 cagctggctg tacagctgat ccagagcagc ctgaccttcc ggtttcaggg ttgctttgtt
    13501 gaagttgaac agaacgtcag acttcagagt gaagtgcttg gtctgtactt ccggtgccgg
    13561 agctggagcc ggagcaacta ctggagctgc ttcgccctga ccgaaacggt aggaaacacc
    13621 caggctcagc atgccgttgt ccggacgagt gccgatggtg tgtgcgtcac cgatgttgtt
    13681 ggtccactgg tattccagac gggtagcgat ttcaggagtg atcgcgtact caacaccgcc
    13741 agcgaagacc ggagaaacgc cggtgtcgtg gtttttacca taaacgttgg atttagtgtc
    13801 tgcacgccat accataccac ccagacgagt gtagatgtcc aggtcgtcag tgattgggta
    13861 acccagttta gcggtcagtt gaacgccctg agctttgtat gcaccgtttt caacgctgcc
    13921 tttgtacggc atacgaccta accagtcgta acccatttca aagccaacat acgggttaac
    13981 ctggtaacca ccaaaagcac cagcgcccag ttggttttca tgggtcgggc cattgttgtt
    14041 gatgaaacca gtgtcatggt actgggacca gcccagttta gcaccagtgt accaggtgtt
    14101 atctttcgga gcggcctgcg ctacggtagc gaaaccagcc agtgccactg caatcgcgat
    14161 agctgtcttt ttcatttttt gcgcctcgtt atcatccaaa atacgccatg aatatctcca
    14221 acgagataac acggttaaat ccttcaccgg gggatctgct caatattaac tctaccgata
    14281 tcttcggctt atgccgagca cccctggcga tgtaaagtct acaacgtagt tgaaaactta
    14341 caagtgtgaa ctccgtcagg catatgaaaa aaaagtcttg tataaggtat gtttaatctt
    14401 ttttgtcagc gacaatttac agaagagaat cgcggaaacc gcttcagaca agcctccgca
    14461 aggaaaatta gtcacgactg aaagcattgg ctgggcgaca aaaaaagttc caggattaat
    14521 cctaaattta cttaatgata caaattagag tgaattttta gcccggaaag ttgtctcgtg
    14581 gcgtgagagg atgcgcttac cggacgcata ataaacccca tagcgttacc ttcatttgcc
    14641 gcatcaacaa gttcagcatg ctcttcttca gtcaaatcat ctgccaacca accgatcacc
    14701 acactgtaat tgcccgtgcg taaagcgcga accattgact ccacagtgtg gcaaggggag
    14761 agctggctaa tctgcattac tttcgttaag ggtagcccag atgcctgaac ccattcccga
    14821 ctcagttttt gttgcggtgt taaccagagt tgccagcgcg attgctgacc gagttgctgt
    14881 aacaatggca acagtagaag ttgcgtcatc atgggctgat cttcgcgata gacaacttca
    14941 ctgataagcc cggctgtagt gttttccgta gagacacgcg caattttact tgctgcggat
    15001 gagaacgacg aagaacgatg tgcatagcct gaagtgtaca taatcaatcc agccctgtga
    15061 gttactgtat ggatgtacag tacatccagt gacaacaaag atcaacccta ttttcggaaa
    15121 gagcctcgca aattttgtcg ttggtgacgg gaaaacataa attaatcttg ccccttaaga
    15181 ataagttgcc tattttcgta gttaacggat ccgttaatgt gaatcattct tttatgttat
    15241 gattttaaaa ggaattttat gaaaagcctc tcctataagc ggatctataa atcacaagaa
    15301 tacctggcaa cgttgggcac aattgaatac cgatcattgt ttggcagtta cagcctgacc
    15361 gttgacgaca cggtgtttgc gatggtttct gatggtgagt tgtatcttcg ggcttgtgag
    15421 caaagtgcac agtactgtgt aaaacatccg cctgtctggc tgacatataa aaagtgtggc
    15481 cgatccgtta ccctcaatta ctatcgggtt gatgaaagtc tatggcgaaa tcaactgaag
    15541 ctggtgcgtc tgtcgaaata ttctctcgat gcagcgctga aagagaaaag cacgcgcaat
    15601 acccgggaaa gactgaaaga tttgcccaat atgtcttttc atctggaagc gattctcggg
    15661 gaggtgggga ttaaggatgt acgggcgtta cgtatacttg gggcaaaaat gtgttggttg
    15721 cgactgcggc agcaaaacag tctggtgaca gaaaagattc tgtttatgct tgaaggtgcc
    15781 attatcggca ttcatgaagc tgcgctcccg gtggcacgcc gccaggagct tgcagaatgg
    15841 gctgactctc ttacgccgaa acaggagttt cctgcggaac ttgagtaatc tggcgttgca
    15901 gacgaccaat ctcaggcagt aatgcaatca ataatccaac ttgttgtacg accagaggtt
    15961 ctttgctgtc tgcccgtggt tcaagttgct gcatccgctg tttcaggcta gctaatgcct
    16021 cattgacgcg ttcttcatca gcaggttgat gatgtaacgc gtcatcaaca tagcaaactg
    16081 cgtcatcaag aaacgccaga atttcaggat tagttaactg ctcccggtga gcaccgaggg
    16141 ctgagatata gctggtaaac gtatggttaa ggcacagcaa ccgaaacgcg gcttcgcgaa
    16201 tttgcggggt aacgttcggc tcgctggaca tatttgatac caccgacgcc agctcagcat
    16261 cacggttgtg tgcatcgcgg cgggcaatac gatacgccag acggttatca cgcccctgat
    16321 ggtattgctc cagtatggca tcgagatacc gacagttggc ctctgtggcg cgttcgagca
    16381 tgcgcggcag attgcgaaac tgccagtcag gccagatgta gctcactgcc gcccacgcaa
    16441 tggcacaacc aatcagcgta tcgattacgc gaggtaacgc tacttcaaaa ccttcaccca
    16501 gtaagttaaa acacagtagc accaaaagtg tgatgaacat cgttgcatga gcgtattgca
    16561 cgttacggaa ggcaaaaaag agcacgccgg taataaccag cagcaccagc tgcccttcca
    16621 gtgatggcac aaaccacagc acaggaatgc caatggcgat acctaccagc gtaccaataa
    16681 tccttaactt caggcggtgg cgcgtggcgt tatagtttgg ctggcagaca aacaaacttg
    16741 tcagcaagat ccaatacccg tgatgcattc cggttatctg aatgatggcg tagccgaagc
    16801 acaacaccag cgacattctt accgcatgac ggaagagggc ggattccggc gtgaagtgac
    16861 ggctaagacg cagccagata tcactcaacc cgtgcgggct gtcatcagcg agctcatttt
    16921 cgtcattatt atggggtagt gcctgggcct gttctgattc aattgttgcc agttgggcat
    16981 caatggcgcg taaattgttc agcaaaaatc ccagtgtttt gagtaaatcg gcgggtgcgc
    17041 cgttatcgcg catccgctcc agcgcagcat caatatgcgt aaaagcgcgc tcaaaatgcg
    17101 gatcatgttg ataaggctga cgcaacaaaa tacagcgtga cagttgctgg cacgcctggc
    17161 cctgcatcga catcagccgc tgaaaacgga acagcacgtc gctgtggcga aaatgttcac
    17221 gcaatgtttg atactgaata tgagaagagc tggcacgctc gtgaatatcc tgtgcgacaa
    17281 agtaataatg cagcgtgcga cgcgttcccc gttgaccacg atcgccacgt aagcgggtca
    17341 gcagcgagag tttcgtctga ttcaatgtcg ccatcagcag accgttggcg agagccaaat
    17401 cgtacagcgg tgcctggctt tgatcttcaa tatcaggatc aaacatgcgc gacttgagct
    17461 caagataacg cgccagttgt tcatagcaac gcgccaggtt gtcctgcagc gggcggaccg
    17521 ggaacagcag atgaccaata agtgtcagga cgttgtacca gacggcaccg gccagcagat
    17581 acatcggctg ctgataccag tgctcataca gtgatgttcc caacatagtg taaatggcga
    17641 tcagcaatgc accgaaggca attgttgcat agcgttgacc cagaccgccg agcaaaatga
    17701 agccgctggt agagagcgtt aagccaatcg caaatagcca gggccaggga aacagcaatt
    17761 ctactgaggc cgaggcgata aaaaagcaga acagcgtaat gatgaggtta cgcaaacgtc
    17821 ccgccagtcg gtcatcgaga tcggtcagcg ccgctgccac catccccagc gttagcggaa
    17881 tcgtcagttt tacatcaccc agccaccacg gaaacgctgt ggttccacaa agcgcaataa
    17941 aaatacgcgc gtaatacagc caggcgctgt tccaggtata gcgtttgagc aaaggactta
    18001 gcataaaggc catattagtg gattattcaa aacgacgacg tgcattggct tcacgcgcag
    18061 cttgcgctgt ttctaccgat accacgcgac gaccaaccgg ccatagtgca atagcggcaa
    18121 ttttaaagtt cgcaatgccg acaggaatgc caatgattga aatacattgt gcgatgcccg
    18181 ttgcaatgtg catcaggcat aaccaccagc caaagaaaat cagccagaaa atattcaata
    18241 ccgtaccgcc agtattcagc agcacatttt tgccagccgg gttcagttca tcgacatgaa
    18301 tagcttcatt gccataaggc accagagaca gtttagtgat ctcccagcag gatcgtgtca
    18361 gcggtaaggt aaaaatcagc acaatactga ccagagtcgc caacagccag cccagagtgg
    18421 tggcaaatcc gccaagcaca aagttcagaa tgttcaaaac ggtacgcata aaacctcgct
    18481 ttactgtggt tttcagtaat gggcggcaat tgtaacgttt ttttgggctg gagcacgttt
    18541 tctctgacgg ttacactgat aagaaataat ttcgtgtgga tctacagagt catggaactg
    18601 aaagcgacaa cgcttggaaa acgtctggca cagcaccctt acgatcgggc ggtgatcctc
    18661 aatgccggga ttaaagtctc cggcgatcgc cacgaatacc ttattccttt caatcaatta
    18721 ctggcgattc actgtaagcg cggtctggta tggggcgagc tggaatttgt actgccggac
    18781 gaaaaagtgg tgcgtctgca cggcaccgaa tggggcgaga cgcagcgttt ttaccatcat
    18841 cttgatgctc actggcggcg gtggagtggc gagatgagcg aaattgcgtc tggtgtttta
    18901 cgccagcaac tggatttgat tgccacgcgc actggagaaa ataaatggct gacgcgtgag
    18961 caaacctctg gcgttcagca acaaatccgc caggctttgt cggcgttgcc gttgccggtt
    19021 aaccgactgg aagaattcga taactgccgt gaggcgtggc gtaaatgtca ggcctggttg
    19081 aaagatattg aaagcgctcg gttgcagcat aaccaggcgt ataccgaagc catgcttacc
    19141 gagtatgcgg attttttccg ccaggtcgag tcttcaccgc tgaatccggc gcaggcccgg
    19201 gcagtcgtta atggcgagca ttctctgtta gtgctggcag gtgcaggaag cggaaaaacg
    19261 tcggtgctgg tggcccgtgc aggctggttg ctggcgcgtg gtgaagcgtc ccctgagcaa
    19321 attttattgc tggcgtttgg tcgcaaagcc gctgaagaga tggacgagcg gattcgcgaa
    19381 cggctacata ccgaagacat taccgcacgc acgtttcatg cgctggcgct gcatattatt
    19441 cagcagggca gcaaaaaagt tccgatagtc agcaaactgg aaaatgatac cgctgcccgt
    19501 catgaactct ttattgctga gtggcgcaag caatgcagcg aaaagaaagc gcaggcgaag
    19561 ggctggcggc aatggctgac ggaagaaatg cagtggtcag tgccagaagg taacttctgg
    19621 gatgatgaaa aattacagcg tcgccttgcc tctcgcctcg atcgctgggt aagtctgatg
    19681 cggatgcacg gtggtgcaca ggcagaaatg attgccagtg cacccgaaga gattcgcgat
    19741 ctgttcagta aacgtatcaa gttgatggcc ccgttattaa aagcctggaa aggtgcgctg
    19801 aaggcagaaa acgctgtcga tttttcgggc cttattcatc aggcgattgt gattctggag
    19861 aaaggtcgct ttatcagccc gtggaagcat attctggttg atgaatttca ggatatctcg
    19921 ccgcagcggg cagcgttgtt agcggcatta cgcaagcaaa acagtcagac gacgttgttc
    19981 gctgttggtg atgactggca ggcgatttac cgattcagcg gtgcgcaaat gtcgctcacc
    20041 accgctttcc atgaaaactt tggtgaaggc gaacgctgtg atttagacac gacttaccgt
    20101 tttaacagtc gtatcggtga ggtggcaaac cggtttattc agcagaaccc aggccagctg
    20161 aaaaagccgc taaacagctt aaccaatgga gacaaaaaag ccgtcacgtt attggatgag
    20221 agtcaacttg acgctttgct ggataagctc tctggttatg ccaaaccgga agagcgcatt
    20281 ctgatcctgg cgcgttacca tcacatgagg cctgccagcc tggaaaaagc ggcaacacgc
    20341 tggccgaagt tgcaaatcga ctttatgacc attcatgcca gcaaagggca acaggcggat
    20401 tacgtcatca tcgttggctt gcaggaggga agtgatggtt ttccggctgc ggcgcgggag
    20461 tcgattatgg aagaggcgct actgccaccg gttgaggatt tcccggacgc tgaagaacgg
    20521 cggttaatgt acgtggcgct gacccgggca cgccatcggg tatgggcact gtttaacaaa
    20581 gagaatccct ctctctttgt ggaaatactg aaaaatctgg atgtgccggt ggcgagaaaa
    20641 ccgtaagaaa caggtggcgt ttgccacctg tgcaatatta cttcagacgg tccgcgagat
    20701 aacgctgata atcggggatc agaatatcga ccgcgtcgtt gaaatgcggc gactggatta
    20761 tgaagtctgc cgttgccacg ttggtggcga ccggaatgtt ccataccgtc gccagacgca
    20821 gcaaggcttt cacgtcagga tcgtgcggca cggcatttag tggatcccag aagaaaatca
    20881 atacatcaat tttcccttct gagatcaatg cgccaacctg ctggtcaccc cccattgggc
    20941 cactcaacat cgcgttgacg ttcatgccgg tcgcgcggga aattaagtta ccggtagtgc
    21001 ctgttgcata cagtacgtgt tgttccagta acggttgatg ccgttccacc cagctcatca
    21061 gcatttgttt gcagtgatcg tgtgccacca gcgcaatatg tttccgcgca ggtaaagtgc
    21121 gagtcgtcag ttccataatg tacatccgta gttaactttc ctacagatta ctgtaagcac
    21181 ttatcgctgc aagataaaga ccgaaaaagc ctgcgcacag gcacaaaaat ctcaggaaga
    21241 tggttgtttt tccgcccact gcaggaaagt atttcgcgtt tgtgggtcag ccagtttaaa
    21301 ccaatacttc agccgttgtt ctgtgagcac ctgagactgc ggtggaatag catccagctc
    21361 ggaaacaccc gaaagcagcg tactgtcatc tgccatcatc gtggcaaatt gcggtagtga
    21421 agcgcgtttc gcggatttgt tatagcgttc aacctcgacc tcataatcaa tcgtttttgc
    21481 cgttgaggta atggcgagaa tatccagctt taccggaatt ggcgtcgcat cgccatccaa
    21541 caattcaagg tgcggcgcgg catcaaaatg gttagcttcc cgctcattct ccaggcgagg
    21601 caggcgaaaa ttgacctggt tgatgagctg ggtattaaaa ctgaccacca gcggtgggga
    21661 gatatacagc cgttcttcac tattggaaag atgaatcgtc ttctcaacgc gaaacactaa
    21721 ctgatgcggt ccattatcca gttcaatgct gtccgcaccg cgcagcagag agctggaaac
    21781 cttcttgccg tcgagtacca gaagatcgac atcggttgaa agccgcagcg tggtggcaaa
    21841 aacggatacc ggcaaacata atgcaatcaa ggtggtcacg atgccggttt tcataggtag
    21901 ctcctgtcaa aagaccgccc gaataataat gtgtcaaaat ttt
//
LOCUS       DAPHBJ010000066        21638 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.066, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000066 DAPHBJ010000000
VERSION     DAPHBJ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.066"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(125..358)
                     /locus_tag="RHZ76_002197"
     CDS             complement(125..358)
                     /locus_tag="RHZ76_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HDV2812838.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLCSEKRVSSEANKEKSDITELLRKQVRPD"
     gene            747..1292
                     /locus_tag="RHZ76_002198"
     CDS             747..1292
                     /locus_tag="RHZ76_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HDV2812839.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1267..3192
                     /locus_tag="RHZ76_002199"
     CDS             1267..3192
                     /locus_tag="RHZ76_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDV2812840.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEVINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFELTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3189..3395
                     /locus_tag="RHZ76_002200"
     CDS             3189..3395
                     /locus_tag="RHZ76_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HDV2812841.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3392..4993
                     /locus_tag="RHZ76_002201"
     CDS             3392..4993
                     /locus_tag="RHZ76_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDV2812842.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     PSRLFRTQFRMVSPKRISNPNNTGDSRYCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGLASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            4974..6293
                     /locus_tag="RHZ76_002202"
     CDS             4974..6293
                     /locus_tag="RHZ76_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HDV2812843.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRQMFAQKVSAYTGLSVQAVLDTEAAVYSGQEVID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTEETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLASGNPASDAVNDLLNTPV"
     gene            6303..6635
                     /locus_tag="RHZ76_002203"
     CDS             6303..6635
                     /locus_tag="RHZ76_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HDV2812844.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSKKTPAMTPLMPDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDEAKKRTAF
                     AGTAISIV"
     gene            6691..7716
                     /locus_tag="RHZ76_002204"
     CDS             6691..7716
                     /locus_tag="RHZ76_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDV2812845.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSEAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     THARGLRTYGCIQDADALSEGINASPRYPKNWKTSGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            7758..8153
                     /locus_tag="RHZ76_002205"
     CDS             7758..8153
                     /locus_tag="RHZ76_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HDV2812846.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGLDTSVSPENALTGHENEVVSAQTDTVTDTAALVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            8165..8518
                     /locus_tag="RHZ76_002206"
     CDS             8165..8518
                     /locus_tag="RHZ76_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HDV2812847.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            8530..9108
                     /locus_tag="RHZ76_002207"
     CDS             8530..9108
                     /locus_tag="RHZ76_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2812848.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSVAQV
                     ARETKVRRKLVKERARLKRATVKNPQARIRVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9105..9500
                     /locus_tag="RHZ76_002208"
     CDS             9105..9500
                     /locus_tag="RHZ76_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2812849.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEADFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDVPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            9508..10248
                     /locus_tag="RHZ76_002209"
     CDS             9508..10248
                     /locus_tag="RHZ76_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2812850.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDKDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GNTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEINVTAS"
     gene            10264..10686
                     /locus_tag="RHZ76_002210"
     CDS             10264..10686
                     /locus_tag="RHZ76_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HDV2812851.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQTEDAGPAEPVSAGKCSMVS"
     gene            10668..11102
                     /locus_tag="RHZ76_002211"
     CDS             10668..11102
                     /locus_tag="RHZ76_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HDV2812852.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPTSPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11095..13656
                     /locus_tag="RHZ76_002212"
     CDS             11095..13656
                     /locus_tag="RHZ76_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDV2812853.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRMLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAVLGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLRSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSLTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            13653..13982
                     /locus_tag="RHZ76_002213"
     CDS             13653..13982
                     /locus_tag="RHZ76_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2812854.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            13982..14680
                     /locus_tag="RHZ76_002214"
     CDS             13982..14680
                     /locus_tag="RHZ76_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDV2812855.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            14685..15428
                     /locus_tag="RHZ76_002215"
     CDS             14685..15428
                     /locus_tag="RHZ76_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2812856.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     DYFRMAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            15365..15997
                     /locus_tag="RHZ76_002216"
     CDS             15365..15997
                     /locus_tag="RHZ76_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDV2812857.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDVSTSGLTAQLHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPTAASTDNGKQN
                     TYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16058..19471
                     /locus_tag="RHZ76_002217"
                     /pseudo
     CDS             16058..19471
                     /locus_tag="RHZ76_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            19542..20141
                     /locus_tag="RHZ76_002218"
     CDS             19542..20141
                     /locus_tag="RHZ76_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HDV2812858.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            20206..>21638
                     /locus_tag="RHZ76_002219"
     CDS             20206..>21638
                     /locus_tag="RHZ76_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HDV2812859.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDAGTSAREAATHATDAAGSARAASTSAG
                     QAATSAQEASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNAAASQKSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASTSASSAASSATAAGNSVKAAKT
                     SETNAKSSETAAEQSASAAADSETAAASSAREASIKAEEAAVSATAAGKSAESAASSA
                     STATTKAGEATEQASAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAARGSATTASTKATEAAGSAAAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNST"
BASE COUNT         4991 a         5484 c         6724 g         4439 t
ORIGIN      
        1 accattgata gtttaaatcg tttcgaactt tatcaaatat ttgtttaaaa atgactacct
       61 gatccataga taaaccttcc atgtgatatg agggggcgta gtctgcacga ttatctaaat
      121 tgcttcaatc tggtctgacc tgttttctga gcaattcagt aatgtcactc ttttctttgt
      181 ttgcttcaga agaaactctt ttttctgagc acagtctccg gcggcaggct tcaatgaccc
      241 aggctgagaa attcccggac cctttttgct caagagcgat gttaatttgt tcaatcattt
      301 ggttaggaaa gcggatgttg cgggttgttg ttctgcgggt tctgttcttc gttgacatga
      361 ggttgccccg tattcagtgt cgctgatttg tattgtctga agttgttttt acgttaagtt
      421 gatgcagatc aattaatacg atacctgcgt cataattgat tatttgacgt ggtttgatgg
      481 cctccacgca cgttgtgata tgtagatgat aatcattatc actttacggg tcctttccgg
      541 tgatccgaca ggttacgggg cggcgacctc gcgggttttc gctatttatg aaaattttcc
      601 ggtttaaggt gtttccgttc ttcttcgtcg taacttaatg tttttattta aaataccccc
      661 tgaaaagaaa ggaaacgaca ggtgctgaaa gcgagctttt tggcctctgt cgtttccttt
      721 ctctgttttt gtccgtggaa tgagcaatgg aagtcaacaa aaagcagctg gctgacattt
      781 tcggtgcgag tatccgtacc attcagaact ggcaggaaca gggaatgccc gttctgcgag
      841 gcggtggcaa gggtaatgag gtgctttatg actctgccgc cgttataaga tggtatgccg
      901 aaagggatgc tgaaattgag aacgaaaagc tgcgccggga agttgaagaa ctgcggcagg
      961 ccagcgagac agatctccag ccagggacta ttgagtacga acgccatcga cttacgcgtg
     1021 cgcaggccga cgcacaggag ctgaaaaatg ccagagactc cgctgaagtg gtggaaaccg
     1081 cattctgtac tttcgtgctg tcgcggatcg caggtgaaat tgccagtatt ctcgacggga
     1141 tccccctgtc ggtgcagcgg cgttttccgg aactggaaaa ccgacatgtt gatttcctga
     1201 aacgggatat catcaaagcc atgaacaaag cagccgcgct ggatgaactg ataccggggt
     1261 tgctgagtga atatatcgaa cagtcaggtt aacaggctgc ggcattttgt ccgcgccggg
     1321 cttcgctcac tgttcaggcc ggagccacag accgccgttg aatgggcgga tgctaattac
     1381 tatctcccga aagaatccgc ataccaggaa gggcgctggg aaacactgcc ctttcagcgg
     1441 gccatcatga atgcgatggg cagcgactac atccgtgagg tgaatgtggt gaagtctgcc
     1501 cgtgttggtt attccaaaat gctgctgggt gtttatgcct acttcataga gcataagcag
     1561 cgcaacaccc ttatctggtt gccgacggat ggtgatgccg agaactttat gaaaactcac
     1621 gttgagccga ccatccgtga tattccttcg ctgcttgcgc tggccccgtg gtatggcaaa
     1681 aagcaccggg ataacacgct caccatgaag cgtttcacca atggtcgtgg cttctggtgc
     1741 ctgggcggta aagcggcaaa aaactaccgt gaaaagtcgg tggatgtggc gggttatgat
     1801 gaacttgctg cttttgatga tgatattgaa caggaaggct ctccgacgtt cctgggtgac
     1861 aagcgtattg aaggctcggt ctggccaaag tccatccgtg gctccacgcc caaagtgaga
     1921 ggcacctgcc agattgagcg tgcagccagt gaatccccgc attttatgcg ttttcatgtt
     1981 gcctgcccgc actgcgggga ggagcagtat cttaaatttg gcgacaaaga gacgccgttt
     2041 ggcctcaaat ggacgccgga tgacccctcc agcgtgtttt atctctgcga gcataatgcc
     2101 tgcgtcatcc gccagcagga gctggacttt actgatgccc gttatatctg cgaaaagacc
     2161 gggatctgga cccgtgatgg cattctctgg ttttcgtcat ccggtgaaga gattgagcca
     2221 cctgacagtg tgacctttca catctggaca gcgtacagcc cgttcaccac ctgggtgcag
     2281 attgtcaaag actggatgaa aacgaaaggg gatacgggaa aacgtaaaac cttcgtaaac
     2341 accacgctcg gtgagacgtg ggaggcgaaa attggcgaac gtccggatgc tgaagtgatg
     2401 gcagagcgga aagagcatta ttcagcgccc gttcctgacc gtgtggctta cctgaccgcc
     2461 ggtatcgact cccagctgga tcgctacgaa atgcgcgtat ggggatgggg gccgggtgag
     2521 gaaagctggc tgattgatcg gcagattatt atgggccgcc acgacgatga acagacgctg
     2581 ctgcgtgtgg atgaggtcat caataaaacc tatacccgcc ggaatggtgc agaaatgtcg
     2641 atatcccgta tctgctggga tattggcggg attgacccga ccattgtgta tgaacgctcg
     2701 aaaaagcatg ggctgttccg ggtgatcccc attaaagggg catccgtcta cggtaagccg
     2761 gtggccagca tgcctcgtaa gcgaaacaaa aacggggttt accttaccga aatcggtacg
     2821 gataccgcga aagagcagat ttataaccgc ttcacactga cgccggaagg ggatgaaccg
     2881 cttcccggtg ccgttcactt cccgaataac ccggatattt ttgaactgac cgaagcgcag
     2941 cagctgactg ctgaagagca ggtcgaaaaa tgggtggatg gcaggaaaaa aatactgtgg
     3001 gacagcaaaa agcgacgcaa tgaggcgctc gactgcttcg tttatgcgct ggcggcgctg
     3061 cgcatcagta tttcccgctg gcagctggat ctcagtgcac tgctggcgag cctgcaggaa
     3121 gaggatggtg cagcaaccaa caagaaaaca ctggcagatt acgcccgtgc cttatccgga
     3181 gaggatgaat gacgcgacag gaagaacttg ccgctgcccg tgcggcactg catgacctga
     3241 tgacaggtaa acgggtggca acggtacaga aagacggacg gcgagtggag tttacggcca
     3301 cttccgtgtc tgacctgaaa aaatacattg cggagctgga ggtgcagacc ggcatgacac
     3361 agcgacgcag gggacctgca ggattttatg tatgaaaacg cccaccattc ccacccttct
     3421 gggaccggac ggcatgacat cgctgcgtga atatgccggt tatcacggcg gtggcagcgg
     3481 atttggtggg cagttgcggg cgtggaaccc accgggtgaa agtgtggatg cagccctgct
     3541 gcccaacttt acccgtggca atgcccgcgc agacgatctg gtacgcaata acggctatgc
     3601 cgccaacgcc atccagttgc atcaggatca tatcgtcggg tcttttttcc ggctcagtca
     3661 tcgcccaagc tggcgctatc tgggcatcgg ggaggaagaa gcccgtgcct tttcccgcga
     3721 ggttgaagcg gcatggaaag agtttgccga ggatgactgc tgctgcattg acgttgagcg
     3781 aaaacgcacg tttaccatga tgattcggga aggtgtggcc atgcacgcct ttaacggtga
     3841 actgttcgtt caggccacct gggataccag tccgtcgcgg cttttccgga cacagttccg
     3901 gatggtaagc ccgaagcgca tcagcaaccc gaacaatacc ggcgacagcc ggtactgccg
     3961 tgccggtgtg cagattaatg acagcggtgc ggcgctggga tattacgtca gcgaggacgg
     4021 ctatcctggc tggatgccgc agaaatggac atggataccc cgtgagttac ccggcgggct
     4081 cgcctcgttc attcacgttt ttgaacccgt ggaggacggg cagacccgcg gtgcaaatgt
     4141 gttttacagc gtgatggagc agatgaagat gctcgacacg ctgcagaaca cgcagctgca
     4201 gagcgccatt gtgaaggcga tgtatgccgc caccattgag agtgagctgg atacgcagtc
     4261 agcgatggat tttattctgg gcgcgaacag tcaggagcag cgggaaaggc tgaccggctg
     4321 gattggtgaa attgccgcgt attacgccgc agcaccggtc cgtctgggag gcgcaaaagt
     4381 gccgcacctg atgccggggg actcactgaa cctgcagacg gctcaggaca cggataacgg
     4441 ctactccgtg tttgagcagt cactgttgcg gtatatcgct gccgggctgg gtgtctcgta
     4501 tgagcagctt tcccggaatt acgcccagat gagctactcc acggcacggg ccagtgcgaa
     4561 cgagtcgtgg gcgtacttta tggggcggcg aaaattcgtc gcatcccgtc aggcgagcca
     4621 gatgtttctg tgctggctgg aagaggccat cgttcgccgc gtggtgacgt taccttcaaa
     4681 agcgcgcttc agttttcagg aagcccgcag tgcctggggg aactgcgact ggataggctc
     4741 cggtcgtatg gccatcgatg gtctgaaaga agttcaggaa gcggtgatgc tgatagaagc
     4801 cggactgagc acctacgaga aagagtgcgc gaaacgcggt gacgactatc aggaaatttt
     4861 tgcccagcag gtccgtgaaa cgatggagcg ccgcgcagcc ggtcttaaac cgcccgcctg
     4921 ggcggctgca gcatttgaat ccgggctgcg acaatcaaca gaggaggaga agagtgacag
     4981 cagagctgcg taatctcccg catattgcca gcatggcctt taatgagccg ctgatgcttg
     5041 aacccgccta tgcgcgggtt ttcttttgtg cgcttgcagg ccagcttggg atcagccgcc
     5101 tgacggatgc ggtgtccggc gacagcctga ctgcccagga ggcactcgcg acgctggcat
     5161 tatccggtga tgatgacgga ccacgacagg cccgcagtta tcaggtcatg aacggcatcg
     5221 ccgtgctgcc ggtgtccggc acgctggtca gccggacgcg ggcgctgcag ccgtactcgg
     5281 ggatgaccgg ttacaacggc attatcgccc gtctgcaaca ggctgccagc gatccgatgg
     5341 tggacggcat tctgctcgat atggacacgc ccggcgggat ggtggcgggg gcatttgact
     5401 gcgctgacat catcgcccgt gtgcgtgaca taaaaccggt atgggcgctt gccaacgaca
     5461 tgaactgcag tgcaggtcag ttgcttgcca gtgccgcctc ccggcgtctg gtcacgcaga
     5521 ccgcccggac aggctccatc ggcgtcatga tggctcacag taattacggt gctgcgctgg
     5581 agaaacaggg tgtggaaatc acgctgattt acagcggcag ccataaggtg gatggcaacc
     5641 cctacagcca tcttccggat gacgtccggg agacactgca gtcccggatg gacgcaaccc
     5701 gccagatgtt tgcgcagaag gtgtcggcat ataccggcct gtccgtgcag gctgtgctgg
     5761 ataccgaggc tgcagtgtac agcggtcagg aggtcattga tgccggactg gctgatgaac
     5821 ttgttaacag caccgatgcg atcaccgtca tgcgtgatgc actggatgca cgtaaatccc
     5881 gtctctcagg agggcgaatg accaaagaga ctcaatcaac aactgtttca gccactgctt
     5941 cgcaggctga cgttactgac gtggtgccag cgacggaggg cgaaaacgcc agcgcggcgc
     6001 agccggacgt gaacgcgcag atcaccgcag cggttgcggc agaaaacagc cgcattatgg
     6061 ggatcctcaa ctgtgaggag gctcacggac gcgaagaaca ggcacgcgtg ctggccgaaa
     6121 cccccggtat gaccgaggaa acggcccgcc gcattctggc cgcagcacca cagagtgcac
     6181 aggcgcgcag tgacactgcg ctggatcgtc tgatgcaggg ggcaccggca ccgctggctt
     6241 caggtaaccc ggcatctgat gccgttaacg atttgctgaa cacaccagtg taagggatgt
     6301 ttatgacgag caaagaaacc tttacccatt accagccgct gggcaacagt gacccggctc
     6361 ataccgcaac cgcgcccggc ggattgagta agaaaacgcc agcaatgact ccgttgatgc
     6421 cggatacctc cacccgtaag ctggttgcgt gggatggcac caccgacggt gctgccgttg
     6481 gcattcttgc tgttgctgct gaccagacca gcaccacgct gacgttctac aagtccggca
     6541 cgttccgtta tgaggatgtg ctctggccgg aggctgccag cgacgaggcg aaaaaacgga
     6601 ccgcgtttgc cggaacggca atcagcatcg tttaacctga cccttcatca ctaaaggccg
     6661 cctgtgcggc tttttttacg ggattttttt atgtcgatgt acacaaccgc ccagctgctg
     6721 gcggcaaatg agcagaaatt taagtttgat ccgctgtttc tgcgtctctt tttccgtgag
     6781 agctatccct tcactacgga gaaagtctat ctctcacaaa ttccgggact ggtaaacatg
     6841 gcgctgtacg tttcgccgat tgtttccggt gaggttatcc gttcccgtgg cggctccacc
     6901 tctgaattta cgccgggata tgtcaaaccc aagcatgagg tgaatccgca gatgaccctg
     6961 cgtcgcctgc cggatgaaga tccacagaat ctggcggacc cggcttaccg ccgccgtcgc
     7021 atcatcatgc agaacatgcg agacgaagag ctggccattg ctcaggtcga agagatgcag
     7081 gcagtttctg ccgtgctcaa gggcaaatac accatgaccg gtgaagcctt cgatccggtt
     7141 gaggtggata tgggccgcag tgaagcgaat aacatcacac agtctggcgt cacggagtgg
     7201 agcaagcgtg acaagtccac gtatgacccg accgacgata tcgaagccta cgcgctgaac
     7261 gccagcggcg tggtgaatat catcgtgttt gacccgaaag gctgggcgct gttccgttcc
     7321 ttcaaagccg tcagggagaa gctggatacc cgtcgcggct ctcattccga actggagaca
     7381 gcggtaaaag acctgggcaa agcggtgtct tataagggaa tgtatggcga tgtggccatc
     7441 gtcgtgtatt ccggacagta cgtggaaaac ggcgtcaaaa agaacttcct gccggacaac
     7501 acgatggtgc tgggtaacac tcatgcacgc ggtctgcgca cctatggctg tattcaggat
     7561 gcggatgcat tgagtgaggg tattaatgcg tctccccgtt atccgaaaaa ctggaagaca
     7621 tccggcgatc cggcgcgaga gttcaccatg attcagtcag caccgctgat gctgctggct
     7681 gatcctgatg agttcgtgtc cgttcaactg gcgtaatcat ggcccttcgg ggccattttc
     7741 tctctgtgga ggaatccatg acgaaagatg aactgattgc ccgtctccgg tcgctgggtg
     7801 agcaactgaa ccgtgatgtc agcctgacgg ggacgaaaga agaactggcg ctccgtgtgg
     7861 cagagctgga agaggagctt gatgacacgg atgacgcagc cggtctggac acgtctgtca
     7921 gcccggaaaa tgcgctgacc ggacatgaaa atgaggtggt atcagcgcag acggataccg
     7981 tgactgatac ggctgctctg gtcacggttg tggcactggt gacgctgcat actgatgcac
     8041 ttcacgccac gcgggatgag gctgtggcat ttgtgctgcc gggaacggcg ttccgtgtct
     8101 ctgccggtgt ggcagctgaa atgacagagc gcggcctggc cagaatgcaa taacgggagg
     8161 cgctgtggct gatttcgata acctgttcga tgctgccatt gcccgcgccg atgaaacgat
     8221 acgcgggtac atgggaacgt cagccaccat gacatccggt gagcagtccg gtgctgtgat
     8281 acgtggtgtt tttgatgacc ctgaaaatat cagctatgcc ggacagggcg tgcgcgttga
     8341 aggctccagc ccgtccctgt ttgtccggac tgatgaggtg cggcagctgc ggcgcggcga
     8401 cacgctgacc atcggtgagg aaaacttctg gatagaccgg atttcgccgg atgatggcgg
     8461 aagctgtcat ctctggcttg ggcggggcgt accgcctgcc gttaaccgtc gccgctgaaa
     8521 gggggatgta tggccataaa aggtcttgag caggccgttg aaaacctcag ccgtatcagc
     8581 agaacggcgg tgcccggtgc cgccgcaatg gccattaacc gcgttgcttc atccgcgata
     8641 tcgcagtcgg tggcacaggt tgcccgtgag acaaaggtac gccggaaact ggtaaaggaa
     8701 agggccaggc tgaaaagggc cacggtcaaa aatccgcagg ccagaatcag ggttaaccgg
     8761 ggggatttgc ccgtaatcaa gctgggtaac gcgcgggttg tcctttcccg ccgcagacgt
     8821 cgtaaaaagg ggcagcgttc atccctgaaa ggtggcggca gcgtgcttgt ggtgggaaac
     8881 cgtcgtattc ccggcgcgtt tattcagcaa ctgaaaaatg gccggtggca tgtcatgcag
     8941 cgtgtagccg ggaaaaaccg ttaccccatt gatgtggtga aaatcccgat ggcggtgccg
     9001 ctgaccacgg cgtttaaaca gaatattgaa cggatacggc gtgaacgtct tccgaaagag
     9061 ctgggctatg cgctgcagca tcaactgaga atggtaataa agcgatgaaa catactgaac
     9121 tccgtgcagc cgtactggat gcactggaga agcatgacac cggggcgacg ctttttgatg
     9181 gtcgccccgc tgtttttgat gaggcggatt ttccggcaat tgccgtttat ctcaccggcg
     9241 ctgaatacac gggcgaagag ctggacagtg atacctggca ggcggagctg catattgaag
     9301 ttttcctgcc tgctcaggtg ccggattcag agctggattc gtggatggag tcccggattt
     9361 atccggtgat gagcgatgtc ccggcactgt cagatttgat caccagtatg gtggccagtg
     9421 gctatgacta ccggcgcgac gatgatgcgg gcctgtggag ttcagccgat ctgacttatg
     9481 tcattaccta tgaaatgtga ggacgctatg cctgtaccaa atcctgtaat gccggtgaaa
     9541 ggtgccggga ccaccctgtg ggtttataag gggagcggtg acccttatgc gaacccgctt
     9601 tcagacgttg actggtcgcg tctggcaaaa gttaaagacc tgacgcccgg cgaactgacc
     9661 gctgagtcct atgacgacag ctatctcgat gataaagatg cagactggac tgcgaccggg
     9721 caggggcaga aatctgccgg agataccagc ttcacgctgg cgtggatgcc cggagagcag
     9781 gggcagcagg cgctgctggc gtggtttaat gaaggtaata cccgtgccta taaaatccgc
     9841 ttcccgaacg gcacggtcga tgtgttccgt ggctgggtca gcagtatcgg taaggcggtg
     9901 acggcgaagg aagtgattac ccgcacggtg aaggtcacca atgtgggacg tccgtcgatg
     9961 gcagaagatc gcagcacggt gacggcgaca accggcatga cggtgacacc cgccagtgct
    10021 tccgtagtga aagggcagag caccacgctg accgtggcat tccagccgga gggcgcaacc
    10081 gacaagagct tccgtgcggt gtctgcggat aaaacaaaag ccaccgtgtc ggtcagtggt
    10141 atgaccatca ccgtgaaagg tgttgctgca ggcaaggtca acattccggt cgtatccggt
    10201 aatggtgaac ttgctgcggt tgcagaaatc aacgtcaccg ccagttaatc cggagagtca
    10261 gcgatgttcc tgaaaaccga atcatttgaa cataacggtg tgaccgtcac gctttctgaa
    10321 ctgtcagccc tgcagcgtat tgagcatctc gccctgatga aacggcaggc agaacaggcg
    10381 gagtcagaca gcaaccggaa gtttactgtg gaagacgcca tcagaaccgg cgcgtttctg
    10441 gtggcgatgt ccctgtggca taaccatccg cagaagacgc agatgccttc catgaatgaa
    10501 gccgttaaac agattgagca ggaagtgctt accacctggc ccacggaggc aatttctcat
    10561 gctgaaaacg tggtgtaccg gctgtccggt atgtatgagt ttgtggtgaa tgatgctcct
    10621 gaacagacag aggacgccgg gcctgcagag cctgtttctg cgggaaagtg ttcgatggtg
    10681 agctgagttt tgccctgaaa ctggcgcgtg agatggggcg acccgactgg cgtgccatgc
    10741 ttgccgggat gtcatccact gagtatgccg actggcaccg cttttacagt acccattatt
    10801 ttcatgatgt tctgctggat atgcactttt ccgggctgac gtacaccgtg ctcagcctgt
    10861 ttttcagcga tccggatatg catccgctgg atttcagtct gctgaaccgg cgcgaggctg
    10921 acgaagagcc tgaagatgat gtgctgatgc agaaagcggc agggcttgcc ggaggtgtcc
    10981 gctttggccc ggacgggaat gaagttatcc ctacttcccc ggatgtggcg gacatgacgg
    11041 aggatgacgt aatgctgatg acagtatcag aagggatcgc aggaggagtc cggtatggct
    11101 gaaccggtag gcgatctggt cgttgatttg agtctggatg cggccagatt tgacgagcag
    11161 atggccagag tcaggcgtca tttttccggt acggaaagtg atgcgaaaaa aacagcggca
    11221 gtcgttgaac agtcgctgag ccgacaggcg ctggctgcac agaaagcggg gatttccgtc
    11281 gggcagtata aagccgccat gcgtatgctg cctgcacagt tcaccgacgt ggccacgcag
    11341 cttgcaggcg ggcaaagtcc gtggctgatc ctgctgcaac agggtggtca ggttaaggac
    11401 tccttcggcg ggatgatccc catgttcagg gggcttgccg gtgcgatcac cctgccgatg
    11461 gtgggggcca cctcgctggc ggtggcgacc ggtgcgctgg cgtatgcctg gtatcagggc
    11521 aactcaacct tgtccgattt caacaaaacg ctggtccttt ccggcaatca ggcgggactg
    11581 acggcagatc gtatgctggt cctgtccaga gccgggcagg cggcagggct gacgtttaac
    11641 cagaccagcg agtcactcag cgcactggtt aaggcgggag taagcggtga ggctcagatt
    11701 gcgtccatca gccagagtgt ggcgcgtttc tcctctgcat ccggcgtgga ggtggacaag
    11761 gtcgctgaag ccttcgggaa gctgaccaca gacccgacgt cggggctgac ggcgatggca
    11821 cgccagttcc ataacgtgac ggcggagcag attgcgtatg ttgctcagtt gcagcgttcc
    11881 ggcgatgaag ccggggcatt gcaggcggcg aacgaggccg cgacgaaagg gtttgatgac
    11941 cagacccgcc gcctgaaaga gaacatgggc acgctggaaa cctgggcaga caggacagca
    12001 cgggcattca aatccatgtg ggatgcggtg ctggatattg gtcgtcctga taccgcgcag
    12061 gagatgctga ttaaggcaga ggccgcgttt aagaaagcgg acgatatctg gaatctgcgc
    12121 aaggatgatt attttgttaa tgatgaagcg cgggcgcgtt actgggatga tcgtgaaaag
    12181 gcccgtcttg cgcttgaagc cgcccgaaag aaggctgagc agcagagtca acaggacaaa
    12241 aatgcgcagc agcagagcga taccgaagcg tcacggctga aatataccga agaggcgcag
    12301 aaggcttacg aacgcctgca gacgccgctg gagaaatata ccgcccgtca ggaagaactg
    12361 aataaggcac tgaaagacgg gaaaatcctg caggcagatt acaacacgct gatggcggcg
    12421 gcgaaaaagg actatgaagc gacgctgaaa aagccgaaac agtccggcgt gaaggtgtct
    12481 gcgggcgatc gtcaggaaga cagtgctcat gctgccctgc tgacgcttca ggcagaactc
    12541 cggatgctgg agaagcatgc cggagcgaat gagaaaatca gccagcagcg ccgggatttg
    12601 tggaaggcag aaagtcagtt cgcggtactg gaggaggcgg cacaacgtcg ccagctgtcc
    12661 gcacaggaga aatccctgct ggcgcataaa gacgagacgc tggagtacaa acgccagctg
    12721 gctgtacttg gcgacaaggt cacctatcag gagcacctga acgcgctggc acagcaggcg
    12781 gataaattcg cacagcaaca acgggcaaaa cgggccgcca ttgatgcaaa aaaccggggg
    12841 ctgactgacc ggcaggcagc gcgggaagcc acggaacagc gcctgaagga acagtatggc
    12901 gataatcctc tggcgctgaa taacgtcatg tcagagcaga aaaagacctg ggcagctgaa
    12961 gaccagcttc gcgggagctg gatggcaggc ctcaggtcag gctggagtga gtgggaagag
    13021 agcgccacgg acagtatgtc gcaggtaaaa agtgcagcca cgcagacctt tgatggtatt
    13081 gcacagaata tggcggcgat gctgaccggc agtgagcaga actggcgcag cctcacccgt
    13141 tccgtgctgt ccatgatgac agaaattctg cttaagcagg caatggtggg gattgtcggg
    13201 agtatcggca gcgctattgg cggggctgtt ggtggcggcg catccgcgtc aggcggtaca
    13261 gccattcagg ccgctgcggc gaaattccat tttgcaaccg gaggatttac gggaaccggc
    13321 ggcaaatatg agccagcggg gattgttcac cgtggtgaat ttgtcttcac aaaggaggca
    13381 accagccgga ttggtgtcgg caacctgtac cgcctgatgc ggggctatgc ggaaggtggt
    13441 tatgtgggcg gtgccggaag tccggcgcag atgcggcggg ctgaaggcat taattttaat
    13501 cagaacaatc acgtggtgat tcagaacgac ggtacgaatg gtctgccagg tccacagatg
    13561 atgaaggcag tgtatgacat ggcccgcaag ggtgcccgtg atgaaattca gacacagatg
    13621 cgtgatggtg gcctgttctc cggaggtgga cgatgaagac cttccgctgg aaagtgaaac
    13681 ccggtatgga tgtggcttcg gccccttctg taagaaaggt gcgctttggt gatggctatt
    13741 ctcagcgagc gcctgccggg ctgaatgcca acctgaaaac gtacagcgtg acgctttctg
    13801 tcccccgtga ggaggccacg gtactggagt cgtttctgga agagcacggg ggctggaaag
    13861 cctttctgtg gacgccgcct tatgagtggc ggcagataaa ggtgacctgc gcaaaatggt
    13921 cgtcgcgggt cagtatgctg cgtgttgagt tcagcgcaga gtttgaacag gtggtgaact
    13981 gatgcaggat atccggcagg aaacactgaa tgaatgcacc cgtgcggagc agtcggccag
    14041 cgtggtgctc tgggaaatcg acctgacaga ggtcggtgga gaacgttatt ttttctgtaa
    14101 tgagcagaac gaaaaaggtg agccggtcac ctggcagggg cgacagtatc agccgtatcc
    14161 cattcagggg agtggttttg aactgaatgg caaaggcacc agtacgcgcc ccacgctgac
    14221 ggtttctaac ctgtacggta tggtcaccgg gatggcggaa gatctgcaga gtctggtcgg
    14281 cggaacggtg gtccggcgta aggtttacgc ccgttttctg gatgcggtga acttcgtcaa
    14341 cggaaacagt gacgccgatc cggagcagga ggtgatcagc cgctggcgca tcgagcagtg
    14401 cagcgaactg agcgcggtga gtgcctcctt tgtactgtcc acgccgacgg aaacagatgg
    14461 cgctgttttt ccgggacgta tcatgctggc caacacctgc acctggacct atcgcggcga
    14521 tgagtgcggt tatcacggtc cggcggtcgc ggatgaatat gaccagccaa cgtccgatat
    14581 cacgaaggat aaatgcagca aatgcctgag cggttgtaag ttccgcaata acgtcggcaa
    14641 ctttggcggc ttcctttcca ttaacaaact ttcgcagtaa tcccatgaca gagacagaat
    14701 cagcgattct ggcgcacgct cggcgatgtg cgccagcgga gtcgtgcggc ttcgtggtaa
    14761 gcacgccgga gggggaaaga tatttcccct gcgtgaatat ttccggtgag ccggaggatt
    14821 atttccggat ggctccggag gactggctgc aggcagagat gcagggtgag attgtggcac
    14881 tggtccacag tcatcccggt ggtctgccct ggctgagtga ggctgaccgg cggctgcagg
    14941 tgcagagtga tttgccgtgg tggctggtct gccggggggc gattcacaag ttccgctgtg
    15001 tgccgcatct caccgggcgg cgctttgagc acggggtgac ggactgttac acgctgttcc
    15061 gggatgctta tcatctggcg gggattgaga tgccggattt tcatcgcggg gatgactggt
    15121 ggcgtcacgg tcagaatctc tatctggata atctggaggc cacagggctg tatcaggtgc
    15181 cgttgtcagc ggcacagccg ggcgatgttc tgctgtgctg ttttggttca tcggtgccga
    15241 atcacgccgc aatttactgc ggcgacggcg agctgctgca ccatattcct gaacaactga
    15301 gtaaacgaga gaggtatacc gacaaatggc agcgacgcac acactccctc tggcgtcacc
    15361 gggcatggca cgcatctgcc tttacgggga tttgcaacga tttggccgcc gcatcgacct
    15421 tcgtgtgaaa acgggggctg aagccatccg cgcactggcc acacagctcc cggcgtttcg
    15481 tcagaaactg agcgacggct ggtatcaggt acggattgcc gggcgggacg tcagcacgtc
    15541 cgggttaacg gcgcagttac atgagactct gcctgacggc gctgtgattc atattgttcc
    15601 cagagtcgcc ggggccaagt cagggggcgt attccagatt gttctggggg ctgccgccat
    15661 tgccggatca ttctttactg ccggagccac tcttgcagca tggggggcag ccattggggc
    15721 cggtggtatg accggtatcc tgttttctct cggtgccagt atggtacttg gtggtgtggc
    15781 gcagatgctg gcaccgaaag ccaggacgcc cacggcagca agtacagata acggcaaaca
    15841 gaacacctat ttctcctcac tggataacat ggttgcccag ggcaatgttc tgcccgttct
    15901 gtacggtgaa atgcgcgtgg ggtcgcgggt ggtctctcag gagatcagca cggcagacga
    15961 aggggatggt ggtcaggttg tggtgattgg tcgctgatgc aaaacatttt atgtgaaacc
    16021 gcctgcgggc ggttttgtcg tttatggagc gtgacgaatg ggtaaaggca gcagtaaggg
    16081 gcataccccg cgcgaagcga aggacaacct gaagtccacg cagctgctga gtgtgatcga
    16141 tgtcatcagc gaagggccga ttgaaggtcc ggtggatgga ttaaaaagcg tgctgctgaa
    16201 cagtacgccg gtgctggaca gtgaggggaa taccaatatc tccggcgtca cggtggtgtt
    16261 ccgggcaggt gagcaggagc agacgccgcc ggagggcttt gaatcctccg gttccgagac
    16321 ggtgctgggt acggaagtga aatacgacac gccgattacc cggaccatca cgtctgcaaa
    16381 catcgaccgt ctgcgcttta ccttcggcgt gcaggcactg gtggaaacca cctcaaaggg
    16441 ggacaggaat ccatcggaag tccgcctgct ggttcagata caacgtaacg gtggctgggt
    16501 gacggaaaaa gacatcacca ttaagggtaa aaccacttca cagtatctgg cctcggtggt
    16561 ggtggataac ctgccgccgc gcccgttcag tatccggatg cgcaggatga cgccggacag
    16621 caccacagac cagctgcaga acaaaacgct ctggtcgtca tacaccgaaa tcatcgatgt
    16681 gaaacagtgc tacccgaaca cggcactggt cggcgtacag gtggattcgg agcagttcgg
    16741 cagccagcag gtgagccgta attatcatct gcgcgggcgt attctgcagg tgccgtcgaa
    16801 ctataacccg cagacgcggc aatacagcgg tatctgggac ggaacgttta agccagcata
    16861 cagcaataac atggcctggt gtctgtggga tatgctgacc catccacgct acggcatggg
    16921 gaaacgtctt ggtgcggcgg atgtggacaa atgggcgctg tatgtcatcg gccagtactg
    16981 cgaccagtca gtgccggacg gctttggcgg cacggagccg cgcatcacct gtaatgcgta
    17041 cctgaccaca caacgtaagg cgtgggatgt gctcagtgat ttctgctcgg cgatgcgctg
    17101 aatgccggta tggaacgggc agacgctgac gttcgtgcag gaccgaccgt cggataaggc
    17161 gtggacctat aaccgcagta atgtggtgat gccggatgat ggcgcgccgt tccgctacag
    17221 cttcagcgcc ctgaaagacc gccataatgc cgttgaggtg aactggattg acccgaacaa
    17281 cggctgggag acggcgacag agcttgttga agatacgcag gccattgccc gttacggtcg
    17341 taatgttacg aagatggatg cctttggctg taccagccgg gggcaggcac accgcgccgg
    17401 gctgtggctg attaaaacgg agctgctgga gacgcagacc gtggatttca gcgtcggcgc
    17461 agaagggctt cgccatgtac cgggcgatgt tattgaaatc tgcgatgatg actatgccgg
    17521 tatcagcacc ggtggtcgtg tgctggcggt gaacagccag acccggacgc tgacgctcga
    17581 ccgtgaaatc acgctgccat cctccggtac cacgctgata agcctggttg acggaagtgg
    17641 caatccggtc agcgtggagg ttcagtccgt caccgacggc gtgaaggtaa aagtgagccg
    17701 tgttcctgag ggtgttgctg aatacagcgt atgggggctg aagctgccga cgctgcgcca
    17761 gcgcctgttc cgctgcgtga gtatccgtga gaacgacgac ggcacgtatg ccatcaccgc
    17821 cgtgcagcat gtgccggaaa aagaggccat cgtggataac ggggcgcact ttgacggcga
    17881 acagagtggc acggtgaatg gtgtcacgcc gccagcggtg cagcacctga ccgcagaagt
    17941 cactgcagac agcggggaat atcaggtgct ggcgcgatgg gacacaccga aggtggtgaa
    18001 gggcgtgagc tttatgcttc gcctgaccgt ggcagcggac gacggcagtg agcggctggt
    18061 cagcacggcc cggacgacag aaaccacata ccgcttcagg cagctggcgc tggggaacta
    18121 caggctgaca gtccgggcgg taaatgcgtg ggggcagcag ggcgatccgg catcggtatc
    18181 gttccggatt gccgcacctg cagtgccgtc gcggattgag ctgacgccgg gctattttca
    18241 gataaccgcc acgccgcatc ttgccgttta tgacccgacg gtacagtttg agttctggtt
    18301 ctcggaaacg cggattaccg atatcaggca ggtagaaacc acagcccgct atcttggtac
    18361 ggcgctatac tggatagccg ccagtatcaa tatcaaaccg ggccatgatt attactttta
    18421 tatccgcagt gtgaacaccg ttggcaaatc ggcattcgtg gaggccgtcg gtcgggcgag
    18481 cgatgatgcg gaaggttacc tggatttttt caaaggcaag ataaccgaat cccatctcgc
    18541 caaggagctg ctggaaaaag ttgatctgac ggaggataac gccagcagac tggatgagtt
    18601 ttcgaaagag tggaaggacg ctaacgataa atggaatgcc atgtggggcg tcaaaattga
    18661 gcagaccaaa gacggcaaac attatgtcgc gggtattggc ctcagcatgg aggacacgga
    18721 ggaaggcaag ctgagccagt ttctggttgc cgctaaccgt atcgcgttta ttgacccggc
    18781 aaacgggaat gaaacgccga tgtttgtggc gcagggcaac cagatattca tgaacgacgt
    18841 gttcctgaag cgcctgacag cccccgccat taccagcggt ggcagtcctc cggtattttc
    18901 cctgacatca gacggaaagc tgaccgctaa aaatgcggat atcagtggta gtgtgaatgc
    18961 gaactcagga acgctcaaca atgtcacgat taaccagaac tgtacgatta agggcatgct
    19021 ggaggcgacc caggtcagag gggatttcgt taaagctgta tcaaaagcct tcccgaaaaa
    19081 agtcggtacg tggggtaaca cggaaacacc aaacggtacg gttacagtca ccatcagcga
    19141 tgatcataac tttgaccgcc agattattat tccgcccatt atttttaacg gtatagcgta
    19201 tgacgatccg gggagcggaa ataacccggg aggcacgcga tacacgggtt atggttttga
    19261 agttcgcaaa aacggcgtat taatcgcatc cagagaaact aaaggggcca ttcccggtag
    19321 ttacagtgca gttattgata tgcctagtgg tggtggtagc gtcactctgg agtttaagat
    19381 tttccagaaa ggcaatcagg gggcaggcaa tatcaccgac tgtacggtga ttgtgaccaa
    19441 aaaagcggct tccggcatca gtattcgttg aaatatttat aaccccaata aatggcgtca
    19501 ggaatgacgc cttttttatt gcagaaaagc gagaggtaat tatgcgtaaa gtttgtgccg
    19561 ccattttgtc cgcagccatt tgtctggccg tatccggtgc gcctgcatgg gcgtctgaac
    19621 atcagtccac gctgagcgcg gggtatcttc atgcccggac caacgttccc ggcagtgatg
    19681 atctgaacgg gattaacgtg aaataccgtt atgagtttac ggatacgctg gggctggtga
    19741 cgtcattcag ctatgcagga gacaagaatc gccagctgac ccattacagc gatacccgct
    19801 ggcatgaaga ttccgttcgt aaccgctggt tcagcgtaat ggcggggccg tctgtgcgcg
    19861 tgaatgaatg gttcagcgcg tatgcgatgg cgggtgtggc ttacagccgt gtgtcgactt
    19921 tctccgggga ttatctccgc gtaactgaca acaaggggaa aacgcacgac gtgctgaccg
    19981 gaagtgatga cggtcgccac agcaacacgt ctctggcgtg gggagctggc gtgcagttta
    20041 acccgaccga atccgtggcc attgatattg cttatgaagg ctccggcagt ggcgactggc
    20101 gcactgacgg tttcatcgtg ggtgtcggtt ataagttctg attagccagg taacacagtg
    20161 ttatgacagc ccgccggttc aggcgggctt ttttgtgggg tgaatatggc agtaaagatt
    20221 tcaggtgtac tgaaagacgg cacaggaaaa ccggtacaga actgcacaat ccagctgaaa
    20281 gcaaaacgta acagcaccac ggtggtggtg aacacgctgg cctcagaaaa tccggatgaa
    20341 gccgggcgtt acagcatgga cgttgagtac ggtcagtaca gcgttattct gttggtggaa
    20401 ggattcccgc cgtcacatgc cgggaccatc accgtgtatg aagattcccg acccggtacg
    20461 ctgaatgatt ttctcggtgc catgacggag gatgatgccc gtccggaggc tctgcgccgt
    20521 tttgaactga tggtggaaga ggtggcgcgt aacgcgtccg cggtggcaca gaacacggca
    20581 gccgcgaaga agtcagccag tgatgccggc acatctgccc gtgaggcggc aacccatgcg
    20641 actgatgctg caggctcagc gcgtgcagcc agcacgtcag ccggacaggc cgcgacttcg
    20701 gctcaggagg cgtcttccag cgcaggaacg gcatcaacaa aggccactga agcatcaaaa
    20761 agtgctgccg ctgcagagtc ctcaaaaagc gcggcggcca ccagtgccgg tgcggcgaaa
    20821 acgtcagaaa cgaatgctgc agcgtcacaa aaatcagcag ccacttctgc atccactgcg
    20881 acgacgaaag cgtcagaagc cgccacttca gccagagatg cggcggcctc caaagaggcg
    20941 gcaaaatcat cagaaacgaa cgcctccaca agcgccagca gtgcagcctc ctcggcaacg
    21001 gcggcaggaa attccgtgaa ggcggcaaaa acgtccgaga cgaatgctaa gtcttctgaa
    21061 acggcagcgg aacagagcgc ctcagctgcg gcagactctg agacagcagc tgcatcatcg
    21121 gcccgtgagg cgtccattaa ggcagaggag gcagcagtca gtgccaccgc cgccggaaaa
    21181 tcggcagaaa gcgccgcatc atccgcttca acagccacaa cgaaggctgg cgaagccact
    21241 gaacaggcca gcgcagcagc gaggtctgct tccgcagcga agacatccga aacgaacgcg
    21301 aaagcgtcgg aaaccagcgc agaatcctca aaaacggctg ccgcatcgtc cgccagttcg
    21361 gcggcgtcat cggcatcatc tgcgtctgct tcaaaagatg aggcgaccag acaggcgtca
    21421 gcagcgaggg gcagcgccac gacggcatcc acgaaggcga cagaggctgc tggcagtgcg
    21481 gcagcggcag cacagagcaa aagtacggcg gaatccgcgg caacgcgcgc cgagacagca
    21541 gcaaaacggg cagaggatat tgcatccgcc gtggcgcttg aggatgcgag cacgacgaaa
    21601 aaggggatag tacagctcag cagtgcgacc aacagcac
//
LOCUS       DAPHBJ010000067        21369 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.067, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000067 DAPHBJ010000000
VERSION     DAPHBJ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.067"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(202..603)
                     /gene="nikR"
                     /locus_tag="RHZ76_002220"
     CDS             complement(202..603)
                     /gene="nikR"
                     /locus_tag="RHZ76_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HDV2812860.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(609..1415)
                     /gene="nikE"
                     /locus_tag="RHZ76_002221"
     CDS             complement(609..1415)
                     /gene="nikE"
                     /locus_tag="RHZ76_002221"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HDV2812861.1"
                     /translation="MTLLNISGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGEKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(1412..2176)
                     /gene="nikD"
                     /locus_tag="RHZ76_002222"
     CDS             complement(1412..2176)
                     /gene="nikD"
                     /locus_tag="RHZ76_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HDV2812862.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2176..3009)
                     /gene="nikC"
                     /locus_tag="RHZ76_002223"
     CDS             complement(2176..3009)
                     /gene="nikC"
                     /locus_tag="RHZ76_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HDV2812863.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(3006..3950)
                     /gene="nikB"
                     /locus_tag="RHZ76_002224"
     CDS             complement(3006..3950)
                     /gene="nikB"
                     /locus_tag="RHZ76_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HDV2812864.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(3950..5524)
                     /gene="nikA"
                     /locus_tag="RHZ76_002225"
     CDS             complement(3950..5524)
                     /gene="nikA"
                     /locus_tag="RHZ76_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HDV2812865.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(5635..6222)
                     /gene="acpT"
                     /locus_tag="RHZ76_002226"
     CDS             complement(5635..6222)
                     /gene="acpT"
                     /locus_tag="RHZ76_002226"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HDV2812866.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPEMPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6277..7326)
                     /locus_tag="RHZ76_002227"
     CDS             complement(6277..7326)
                     /locus_tag="RHZ76_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDV2812867.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            7458..8675
                     /locus_tag="RHZ76_002228"
     CDS             7458..8675
                     /locus_tag="RHZ76_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2812868.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADSLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(8679..9236)
                     /gene="dcrB"
                     /locus_tag="RHZ76_002229"
     CDS             complement(8679..9236)
                     /gene="dcrB"
                     /locus_tag="RHZ76_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HDV2812869.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(9309..9974)
                     /locus_tag="RHZ76_002230"
     CDS             complement(9309..9974)
                     /locus_tag="RHZ76_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HDV2812870.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10195..10440
                     /gene="tusA"
                     /locus_tag="RHZ76_002231"
     CDS             10195..10440
                     /gene="tusA"
                     /locus_tag="RHZ76_002231"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HDV2812871.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(10542..12740)
                     /gene="zntA"
                     /locus_tag="RHZ76_002232"
     CDS             complement(10542..12740)
                     /gene="zntA"
                     /locus_tag="RHZ76_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HDV2812872.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(12814..13440)
                     /locus_tag="RHZ76_002233"
     CDS             complement(12814..13440)
                     /locus_tag="RHZ76_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HDV2812873.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLVGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            13581..13940
                     /locus_tag="RHZ76_002234"
     CDS             13581..13940
                     /locus_tag="RHZ76_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HDV2812874.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(13943..14212)
                     /locus_tag="RHZ76_002235"
     CDS             complement(13943..14212)
                     /locus_tag="RHZ76_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HDV2812875.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14202..14798)
                     /gene="rsmD"
                     /locus_tag="RHZ76_002236"
     CDS             complement(14202..14798)
                     /gene="rsmD"
                     /locus_tag="RHZ76_002236"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HDV2812876.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAEATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            14948..16444
                     /gene="ftsY"
                     /locus_tag="RHZ76_002237"
     CDS             14948..16444
                     /gene="ftsY"
                     /locus_tag="RHZ76_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDV2812877.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQSVVEEIVQVQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAGVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVVEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            16447..17115
                     /gene="ftsE"
                     /locus_tag="RHZ76_002238"
     CDS             16447..17115
                     /gene="ftsE"
                     /locus_tag="RHZ76_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDV2812878.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDLNLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17108..18166
                     /gene="ftsX"
                     /locus_tag="RHZ76_002239"
     CDS             17108..18166
                     /gene="ftsX"
                     /locus_tag="RHZ76_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HDV2812879.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFNINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            18411..19265
                     /gene="rpoH"
                     /locus_tag="RHZ76_002240"
     CDS             18411..19265
                     /gene="rpoH"
                     /locus_tag="RHZ76_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HDV2812880.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            19536..20639
                     /gene="livJ"
                     /locus_tag="RHZ76_002241"
     CDS             19536..20639
                     /gene="livJ"
                     /locus_tag="RHZ76_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HDV2812881.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARSYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(20838..21221)
                     /gene="panM"
                     /locus_tag="RHZ76_002242"
     CDS             complement(20838..21221)
                     /gene="panM"
                     /locus_tag="RHZ76_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HDV2812882.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EERGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         5093 a         5629 c         5905 g         4742 t
ORIGIN      
        1 gtaaaatcct tttctattat tttaatgaca gtatcttcat acgaaaaagc gaagaaaaac
       61 ttgtggtcac aatgaattga agatttttag gaatatattt agaacgttac aaaagagaag
      121 gttcggatag ggtatttaga gaggaatcag gtgtgctagt ggccctttgt tgatcgttca
      181 ccaaaggacc caataataga ctcaatcttc cttcggcaag cactgcaaat gcccgtgccg
      241 cacgccgcgc tgggcgataa cgtcatcggc aaaatgctgc acgtcaccca tgtcaccttt
      301 caacacggcg atttccagac agtcgtcgtg gttgatgtgc acatgcagcg tggcgacgga
      361 gaggtcgtgg tgatgatgct gggtggaaac aatacggctg gctaagtcgc gtttttcgtg
      421 ttcatacaca tacgacagca ccgcgaaacc ttgtgtgccg tgctgctggg tagcttcctg
      481 cgccagggcg ctacgcagaa tatcgcggat agcttcggaa cggttgttat aaccacgacg
      541 ctggctcagg ctgtccagcg tctccagtaa atcgtcatca agcgtgatgg tgactcgttg
      601 catttgcgtt aaaccttttc tgtggtgcga cggcgcacgg ggaatgcggg taataccgcg
      661 ttttgtagca cacgtccggc gtcagaggaa aaggttaatt tctctcccac cacctgggtt
      721 tcgacgattt gtccgttgtc cataaccatt acccgctggc aaaagcgttc taccaggcgt
      781 aagtcgtggg tgatgaacag gcaggcggtg ccaaactgtt gttgtagctt tttcagcagg
      841 cgaatgacac ccgcctgtaa cacgagatca aggttagaaa cggcttcatc cagaatcagt
      901 agtttcggtt cgaccgccag cgcgcgagcc aggcagacgc gctggagctg gccgccgctt
      961 aactgcggtg ggcgtttgtc gagaacgctg tcatcgagat cgaccgcctt cagcatttcg
     1021 ctggcgcgcg ccagttgttc ggattttttc agtgatagca ggtggcgcat cggttcacgc
     1081 aggatctcgc gcacggtttt gcgcggattc acggcgctga tggagtcctg aaataccatc
     1141 tgaatatcgc ggcggaacgc tttacgctgg gcgcgattga gtttcgccag cggttcgcca
     1201 cgccagctaa tattcccctg cgcgggcgat tctaaaccca ccagcaaccg cgcgagggta
     1261 cttttcccgc agccgctgcg ccccagcagg gcgacggttt cgccgctttt cagggtcagg
     1321 gaaacgttat tcagcaccgc ctgatgttga tgttttccgt taaatccacc gtgcgcatag
     1381 tgatgggaaa ggccggagat gttaagtaaa gtcatgatgc cagctccata ccgtagaggg
     1441 cgagatgagc ggaaaccagg ctgcgcgtca ctgtatgttt gggggcgtta aacagcgttt
     1501 ctacatcgcc ctgttcgaca atcttaccgt cagacatcac cgccacgtca tccgccagac
     1561 gcgccacaac gcccatatca tgggtcacca gcagcattcc cggcgcttgt ttttgcataa
     1621 tgctttccag cagatcgagg atgcgcgcct gtgctaccac gtcgaggtcg gtggtcggtt
     1681 catcggcgat gataaacggt gattcacaca gcaccgccat cgcaatcatc atgcgctgca
     1741 acatgccgcc gctcatctcg aacgggtaca gcttcagcac gcgcgcggcg ttttccagcc
     1801 ccaccgcttc tatggcagcg gtaagcgtag cgtcatcggc gggtttccct aacgccaggc
     1861 aggtttcccg cgcgtgggtg tgcatggtgt gcagtggatt aaaggcgctg cgcgggttct
     1921 gcatgatggt ggcaattttg atgccgcgca gggcgcaagg cgaaaccggt ttgccatcgg
     1981 ctaaaatttc ccccgccgtc tggcgaacgc cagcgggcaa aatgcccagc gtcgcggcgc
     2041 aggttaatga tttcccgctg ccgctaccgc cgactaacgc cagcacgcgc ccgcgttgca
     2101 gggttaacga tacaccgtgt accagcggct gcgcggcctg tagcgcgata ttacgtagtt
     2161 caatctgttg cggcattagt gtgcgtgctc cgtcaccaga tgagggtcca gatgatcgcg
     2221 cagtgcgtca cccaccaggt taaaggccat cacactgata aacagcgcca gccccggcca
     2281 gaacatttgc agcggctggg tccagatata ctggcgcgcg tcgttaatca tcacgcccca
     2341 ttcggcggtc ggcgcggtca caccgaggcc aaggaaagac atccccgcga cgtgcagcat
     2401 catatggccg atatccagcg ttgccagcac cagcagcgaa gggatcaccg cgcctgccag
     2461 atgatcgaca aacacccgca catggcccgc accggaaagc cgtgacgcca gcacaaactc
     2521 gcgttggcgt agtgaaatca ccaggctgcg caccatgcgt gcataccacg cccagtgcga
     2581 cagggcgatg gcgataatta cgttggtcag cccggtgccg agcacgccaa ccataaagaa
     2641 cgacagaatc gaggtcggga aggtcataaa catatcggcg acgcgcatgg tggcctgatc
     2701 aacgcgcccg ccaatcaacc cggcgctgcc gccaataacc agccctaatg tcagcaccag
     2761 cagcaggcag gccattaccg aaccgagcga cacgcgggtc gctgccatca gccgcgagaa
     2821 aatatcgcga cctaagtgat cggtgcccag ccagtgctgc gcatccggcg aaagcaggcg
     2881 cgacggcaaa tcaatcgcct gggggtcata cggcagccac cactggctgg tgagcgcaat
     2941 cagcgccagc agggcgataa tgatcagcgc caggcgtacc gaccagcggg aagagaggaa
     3001 aaagttcacg cgtgcgctcc ttcatgacga cgaatgcgcg ggtccagcgc ggcgttgagc
     3061 aaatcgacaa tcaaattaca gaccacaaaa accaccacca tcatcagcgt aaagcactgg
     3121 atcaccggat agtcacggtt aaaaatcgcc gacaccgcat agcgcccgac gcccggccag
     3181 gcaaagatgt tttcgataat catcgtcccg ccaatcagtt cgccgatatg catccccacg
     3241 gcggtgatca tcggcagcga ggcattgcgc aggatgtggc gacgttcggt ctgtttgtcg
     3301 ttcaggccgc gcagacgcgc ccaggtgacg tgacgctgac cggcgacgtc cagcatactg
     3361 gcgcgcagta aacgcgcgtt aatcgccagc gacataaagg caatggaaac cgcaggcaaa
     3421 atgatgtgct gccagccgcc gtaacccatc gcgggtagcc attgcagata caccgaaaac
     3481 gccatcacca gtaaaaacgc cagccagaag ttaggcatcg acacgccaag aaacgcgatg
     3541 aaacgtacgg cgaaatccgg cagacggtcg cgatggcgcg ccgcccagat accgagcggt
     3601 acggaagtga gcagaattaa taccagcgcc gcacctgcaa gttccagcgt ggcgggcagg
     3661 aagttcagca tatcgtccag taccgggcgt tggctggcga atgagatacc aaagtcaaga
     3721 tgcagcgcct tccacaacca ggtgccgtac tggacgtaca gcggctgatc cagtcccagc
     3781 atggtgcggg tagaggccag catctccggc gtcggcggca ggttagacag acgcaaataa
     3841 tcgagcgccg ggtcgccggt accgaggcgc agcatcagaa aaatgatcac cgaggcggcg
     3901 agcaccatcg ggatcagcag cagaaagcgg cgtaatacgt aacgcaacat taaggtttca
     3961 ccggtttaat ctgttcgaac ggaatttcgg tggcgatcgg cgcgtagggg atgttaccca
     4021 gctccggttt tgataccacc atcattgaga tgtaactgat aggcagataa accgcctcgt
     4081 catgcagacg ggtcagaatg tcgcgataca gcgcctgacg ttgcgtttcg tcatgggtcg
     4141 ccagcacttc gccgatctct ttatcaatca gcggtttgtc ggctaatcct tgctgtgcct
     4201 ggaagtcagc gtgtgacggt acgcgcattg aactgaggaa ggcgtgtgga tcatatggcg
     4261 cgccccaggt gcggtggaaa atcatgccaa aacgaccgtc gcgctgacga gcatagatac
     4321 tgctctcttc ttcgccaatc agcgagacat ctgcgccaat ctggcgcata tcagcctgaa
     4381 tgatttccgc catcgatttg cttaacgcat cggtgccgat gaacgaaagt tcaatgcgca
     4441 gcggctgacc atttttctcg cggatgtctt tgcccgcagg cagcgtccaa ccggcttttt
     4501 ccagcaacgc tttcgctttt tgcggatcgt actggcgcgg tttcaggccg aggttggcgt
     4561 agggcacaga aggggcaaac agggtgtcgg cgacctgctg ggtgccatac aacgcgttat
     4621 caatcagcga ttttttgttt accgcgtaat taagagcttc acgtactgcc agctcgttgg
     4681 tgggggcttt ggcggtattg agcgccagca tcacggtttc gatcggctgt gacagttggg
     4741 tgtggtaagc cggattctgg ctaaagcggg cgaaggtatc gagcggtaat aacccttcgt
     4801 ttccgtacag caggtcgata tcgccagttt caaacgccac cgcgcgggta gtcgggtccg
     4861 ggatgacgtt aaaggtgatc tttttaatcg ctggcttttc gccccagtag ttttcgttac
     4921 ggacgaagac atcgtactga ttcagtttcg attcctgcaa aatccacggt ccggtgccaa
     4981 tcggcgcttt aattccgttc atggtttcat ggtttttaaa ctgcgaggga gcgataaaac
     5041 ggaaaggacg gggcagggcc agttcttgca ggaaaggata gtaggcgctt ttcagggtaa
     5101 tttgcagctc tgttttactg agtgctttaa catcaacaat ctggtttgcc agctccagcc
     5161 aggcgtgacg ttgacggtta tcgagcactg cgcggaagtt ttctgccgcc gcctcggcat
     5221 cgaacggttc accgttggag aatttcacgt catcacgcag ggtgaaggtc caggttttac
     5281 catcttctga atgagtccag ctttttgcca gccacgggat caccgaaccg tctgcctgat
     5341 atttcaccaa tggttcataa accatgctct gggcgaacat ctggttaggc gtgtaaaggt
     5401 gcgggtttag tggcccgaca ttcaccggcc aggcggtggt gatttcatct ggtgcagcgg
     5461 catggacgat aaaagacgca caagccagca gcgcaaatag agtgcggcgg agtgtggaga
     5521 gcatggtaac cccaatggat taaaatagat ggcggaaata agtatgacga ttttaagtat
     5581 tcgtcatact gattacctgt tctggatcaa tagatgggca gtcgggtggg cggatcagtt
     5641 aactgaatcg atccattgca cactgtcggc ggtgagcgta aagggagtag gggtgcagat
     5701 cgcaaggctt aaattttcaa gctgacaatg gctgactgac agcgaggagt gataggtgct
     5761 gtctacgctg acgatttgcc aggcgctgcc gccacgctgt ttaacgatgg cttctttgcg
     5821 cgtccagatg cgccagaaca tttccagttg ctgatcagga tgcacggcgt ccatctcagc
     5881 gtgttccccg aggctgaata cggcgttcgc cagccagcgc cagttggcgc gcgggcgaat
     5941 cacttcgata tcgcagccga cttcgccttc atcactcaac agcagggcga tatcgtcacc
     6001 gctatggctt aagttgaacc atagcggcat ttccggcgca aatgcaggtt tgccttgttc
     6061 gccatagatg atctccggta gcggggaaag cgtgtgcgaa agcaatgcac gccccgccag
     6121 ccagcgttcg cgtcgtggac cttgcggtgc ttgctcgcgt aaacccggtg gcagtggagc
     6181 tgcgcttaag gtcgaaactt tccccagaac tatccgatac atatcagggc caacgtttaa
     6241 tggaaaatga aagtgcgtat cgtatcactt gtcgcctcat cccggtaacc gacttttcgg
     6301 tctgcccggc cccagtaaaa tcgccagttt gctaccgcct ttggtggttt ccatccagat
     6361 tttacacacg ctggttaatg gcaccgaaag tagcatccct accgggccga gcagccatcc
     6421 ccaaattaac aatgaaagaa ataccaccat ggtggacatc cccaggcgat ggcccatcat
     6481 ccgtggttct aaaatattgc cgatgaccat atggaccact aaaaacaatg cgccgaccag
     6541 aatacattcg taaacaccat taaacagcag cacctgaatc attggcggta cggcggaaat
     6601 taccgcgccg atattgggca cgtagttgag caaaaacgcc agtactgccc acatcagcgc
     6661 aaactgcacc cccatcagct ccagccccag ccagacgatg acacctgtcc ataaactgag
     6721 tagcgtcttc aatgcaagat agtgcgaaac gcctttaagt gcgcggtgta atcccgcgat
     6781 gtgaatctgt ggattattca gcgcaaaacg cattttgtaa gggacgtggc gcacttcaaa
     6841 cagcataaaa actacggtca tcaccagcaa aagcacgctc gccattgccc cggaaagccc
     6901 ggtcattagc gctgtggtga aggtaaccac tttttccgag tccatccgct gcagcattcg
     6961 ctccggcgac atatgcaaat taagaaaagg caacatctcc tgcaatttaa aaagtttgcg
     7021 cgtcagctcc ttattaaact tcggcagcat agagataaat tcgttaaacg atgccgccag
     7081 tacgccgacc agcgcggtta gtgcgatcag catcaccacc actacaatcg taatggcaac
     7141 ggggcgttgt actccccgac gaataaacca ggtgacgagc gggttgagga cgatggcaaa
     7201 aaacagcgcc agtaacagct gcacaatgat atctgccgct gcgtgaatgc ccgcgaggat
     7261 cactaccagc gaggccagct tgagcagaat gtgcatgccc gttttatcgg gttgaggggt
     7321 ttccattggg gcttccttgt gactttttgt attaagtgta gcgggagtca cgccagcaat
     7381 attctgattc ttactgctga ttttcacgtc agcacgtggt aaaaatgaaa cactgttgta
     7441 aaaatgtggt gatcctcatg cccgaacccg tagccgaacc cgcgctaaac ggattgcgcc
     7501 tgaatttgcg cattgtctct atagtcatgt ttaacttcgc cagctacctc accatcgggt
     7561 tgccgctcgc tgtattaccg ggctatgtcc atgatgtgat gggctttagc gccttctggg
     7621 caggattggt tatcagcctg caatatttcg ccaccttgct gagccgccct catgccggac
     7681 gttacgccga ttcgctggga cccaaaaaga ttgtcgtctt cggtttatgc ggctgctttt
     7741 tgagcggtct ggggtatctg acggcaggat taaccgccag tctgcctgtc atcagcctgt
     7801 tattactttg cctggggcgc gtcatccttg ggattgggca aagttttgcc ggaacgggat
     7861 cgaccctatg gggcgttggc gtggttggct cgctgcatat cgggcgggtg atttcgtgga
     7921 acggcattgt cacttacggg gcgatggcga tgggtgcgcc gttaggcgtc gtgttttatc
     7981 actggggcgg cttgcaggcg ttagcgttaa tcattatggg cgtggcgctg gtggccattt
     8041 tgttggcgat cccgcgtccg acggtaaaag ccagtaaagg caaaccgctg ccgtttcgcg
     8101 cggtgcttgg gcgcgtctgg ctgtacggta tggcgctggc actggcttcc gccggatttg
     8161 gcgtcatcgc cacctttatc acgctgtttt atgacgctaa aggttgggac ggtgcggctt
     8221 tcgcgctgac gctgtttagc tgtgcgtttg tcggtacgcg tttgttattc cctaacggca
     8281 ttaaccgtat cggtggctta aacgtagcga tgatttgctt tagcgttgag ataatcggcc
     8341 tgctactggt tggcgtggcg actatgccgt ggatggcgaa aatcggcgtc ttactggcgg
     8401 gggccgggtt ttcgctggtg ttcccggcat tgggtgtagt ggcggtaaaa gcggttccgc
     8461 agcaaaatca gggggcggcg ctggcaactt acaccgtatt tatggattta tcgcttggcg
     8521 tgactggacc actggctggg ctggtgatga gctgggcggg cgtaccggtg atttatctgg
     8581 cggcggcggg actggtcgca atcgcgttat tactgacgtg gcgattaaaa aaacggcctc
     8641 cggaacacgt ccctgaggcc gcctcatcat cttaaaactt actgaataac cagcgtatta
     8701 atgatgtttt ctgcggtggt ctgcgctttt tgctgatcgt cagcgggcag cgtaatttgc
     8761 atggtcagca gttgattacc cacgttaccc agaataacgg aagagtacgc cgtctggcct
     8821 ttcgcggaga taatactgtc taactgctgc attttgtgac ctttcagctc aatggcttta
     8881 ttggttacca cttgcagctg cggatcgcgg ctacgttgct gatcttccag acgcttcgcc
     8941 agcaccgcca gatcttcttt cggatcatcg cccatgatga cgatgactgc tttctgcccg
     9001 gtggcgtcgg accagacatg catgttattg gcctgcgttc ccagcttacc gctctggtcg
     9061 gtcatatccg ctggcagcga gaaacttaac ttgccatcaa gcaggttgac gggattcccg
     9121 gtagcgttac tttccgcgac cgaaccctgc gccgtagcgt tagtgtcttt atcatcacag
     9181 gccgcaagcc ccataaccag caggccaatt ccgacatatt taaccagatt gcgcattgac
     9241 ttcttccttt cgataaacgg ccataacggc tcattcatcc atcttatcac aactctgata
     9301 acgaaccttt aactcgcctg caaagcgttg atttcggatt tatctgccag tcttttcaac
     9361 agcatattga gtaatacgcc atacattggc aggaagaaaa cgatactgat taacactttg
     9421 aaacagtaat cgaccagcgc gatttccatc cagtgttcag ccataaaggc atccgggcta
     9481 cgccagaagg caatgaagaa aaaggccagc gtgtcgctga cgttaccgaa cagtgtggac
     9541 gctgtcggtg ccagccacca gcggcgactc tgacgcaggc ggttaaaaac gtgcacgtcg
     9601 aggatttgcc ccagcgcgta ggccatgaaa ctggcggtgg cgatacgggc gacaaacagg
     9661 ttgaagtggg cgagtgcgcc gaatccctgc caggaaccca tatagaatag cgacgagatg
     9721 acgtaggaga ttaataacgc agggatcatt accgcgaaga taatgcgtcg ggccagcggt
     9781 gcgccaaaaa tacgcacggt caggtcggta gcaagaaaaa taaacggaaa gctaaacgcg
     9841 ccccaggtgg tatggaaacc caaaatggag acgggaagct gaaccagata gttactggag
     9901 gtgatcacca gcagatgaaa taacgataac cagaacaacg ccttatagcg ttgagtttgc
     9961 gagaaaacgt tcatattgta cctttttgat taaccattgg ggtgagggaa cccaatacgt
    10021 acgacacgtc tgttacctta acataataac gtgcttaccc ttttttcgag ccgccgcatg
    10081 atactgcttt acgttgacaa tgcaatggtt aattttcacg caatcgttaa cctggtttgc
    10141 ttacggactc acagggcgta aactagcgcc gtttttttat gtgatgagaa gaaaatgacc
    10201 gatctctttt ccagccctga ccacacactc gacgcacttg gcctgcgctg cccggaaccc
    10261 gtaatgatgg tgcgcaaaac tgtgcgcaat atgcagcccg gcgaaacgtt gctgattatc
    10321 gccgacgatc cggccactac ccgcgatatt cctgggtttt gtacctttat ggaacacgaa
    10381 ctggttgcga aagagacgga tggactgcct tatcgttatt tgattcgtaa aggcggttga
    10441 tgggggctga ttggcttcga tgccgccttt tcccctcacc ctaaccctct ccccagaggg
    10501 gcgaggggac cgatcgcgct caatgttgcg atcggtttgc cttatctcct gcgcaacaat
    10561 cttaacgcat tcgctgtcac cagcaccgtc gcacccgtat ctgccagcac cgccagccat
    10621 agtccggtca tccctaaaag cgtggtgacg aggaagatcc ctttcagtcc cagcgcaatg
    10681 gtgatgttct ggcggatatt ggcgtgagtg gcgcgtgcca gttcaatcat ttgcaccagg
    10741 ccgcgcaggt ggttatgggt taatgctgca tcagcggttt ccagcgccac gtctgtgcca
    10801 ctgcccattg caatcccaat ggcggcagct ttcatcgctg gcgcgtcgtt aataccgtca
    10861 ccgaccatcg ccagcggcgc atgttgattc agctcggtca ccgctttgac tttatcttcc
    10921 ggcaacaggc ccgctttaaa ctccagcccc agctccccgg caattgccgc cgctgcgcgt
    10981 ggattatcgc cggtgaggat cacccctttg acgcccagcg cgttcagttc actgatggca
    11041 gttgcagcat cggcgcgcag ggtatcctgt aacgcaatga caccaagcac gtcatcgtta
    11101 cgtactacca gcactaccgt ttgcccggcg ctttccagtt cgttaattaa accagcaaat
    11161 gcatcagcgg gatgtttccc ggcagcgcaa atcaatacgc gctcaccgtt aacctgcgct
    11221 tcaatgccag acccgaccag cgcccgctgt gattcggcgg cgggaatggc gagtgcagca
    11281 acctgtgctt cgcgcacgat cgcctgcgcc agtggatgcg tcgcgccttg ctcgaccgcc
    11341 gccgccagtg tcagcagttc agattcacta atacccgttg ccggatgaat cgcggtaacg
    11401 cgcggtttac cgacggtcag cgtaccggtt ttatcaaacg ccacctgggt aacacgacct
    11461 agctgctcca gcgccgcacc gcctttaatt aacgccccac gacgtgccgc tgctgccagc
    11521 ccggaggtaa tcgccgcggg cgtggagata actaacgcac acgggcagcc aatcagcagc
    11581 agcgtcagcc ctttataaat ccactcttgc cagctggcgg caaacagcag cggcggcacc
    11641 agcgttacca gcagggcgac ggccataatt gctggcgtat aaatacggct gaaacggtcg
    11701 ataaaccgct caatgggggc gcggcgctct tcggcttctt cgatcagttt cagaatccgg
    11761 tcaatggcgc tggcacccgg ttctgacagc acttccagcg ttaccagacg gtctacgctg
    11821 gtggctcctg caggaacttt atcgcccgtt gcgcgctcca ccggaatgga ttcgccggtc
    11881 agggcgcttt catcaaaact ggcaaagggc gagagcagtt taccgtcggc aggcaaacgc
    11941 ccgcccgccg caacttcaat cacatcgcca gggcgcaggc tgttaatcgc cacctcttcc
    12001 cgctcaccgt tacgcaggcg cgtggcggtt tctggtttca gcgccattaa cgcgctaacg
    12061 ccctgacgcg cgcggctggc ggcccagcct tccagtcgtt caccaatcaa aaacagcagc
    12121 aacaccatcg cagcttcagc cgttgcgcca ataaacagcg caccaatagc agctacgctc
    12181 attaacgttt caatggcgaa gtaactgcct gatttgatca gccgtaatgc ctgacgagca
    12241 atcgggtaca gcccaaccag cgtggtcgcg ataaacgcca gttgcccgaa cggatgattg
    12301 aactgctcca gaccccagct gattgccatc atcacgatta gcgtaatcag cggcagattc
    12361 tctttcaggc gcgatgcttg tggctcgtcg gcggcctgct catcacgcag ggaatagccc
    12421 gctttttgca ccgcagattc aacttgtgca cggatgtcat tgtcggcatc aaccaccagt
    12481 ttttcggtgg cgaacagtac ctgcacctga ttcacgcctg caagctggcg cacggcattt
    12541 tctaccttgc gcgcacaggc ggcgcagtcc atgccgctga ctttccagct atagcgggtg
    12601 ccggagacgt tttcagagag cgttggcgtg ctggaacatg cgccgtcgca gcaacagtcg
    12661 ttggcgttct gtaccgtggt tagcggtttg aacgcggcaa attgaggggc tttcttgccg
    12721 tgattgtcag gagtcgacat ggcatcctcc ggttaagttt tttctcatta accgaaggat
    12781 acactctgga gtcgactcca gagtcaagtt ttatcagaga tacagcgagc ggacgatgag
    12841 gaagtgaccg gcaaagtagc aggccgcagc aatcgcgtta tccgcacgga agcgtcggcg
    12901 atagtggcta cccagccaga caaagttact gataaacagc aacgaagcgc cgacaaacgc
    12961 agagagcgcc ggagcggtcg gacggaagaa ccacagttca cctaccagcc acaccatcac
    13021 cagcgtcatg ccgataaacg tgcagatagg ccaacgatac tcttccagcc gcgtccagat
    13081 aatcgccagt aacagcgcgc ccagaaccag cagtaccaac ggcagaggcc agaagaaaga
    13141 gagcgtcatc tgactggcga aatagatggt gtacagcagg tgcgagagga aaaacgcgcc
    13201 gatggcgtac atcagacgtt gacgtggcaa cagggttagc gcatcgccca gcagtgaggc
    13261 gcacagcccc gccagcacca gatagctaat ggcgtcgaac atcggcgctt gccaggccag
    13321 taacaacagg agaagaaggg ttaacggttt aaacacccag cgttgccagg ttggcccacg
    13381 atacgatgca tccacagata gccatgcgga aagacagaca gcgataaacg accaaagcat
    13441 cttagttcct tgaattcgtt attttttcgt aagcggaatg cctacggtct tgcttccagt
    13501 ttagtggcga gggtggacgg atgacaacac cataactggc agggtatgct tatttccgca
    13561 ttttccgatg agggataaag atgagcaaac cacctctttt ctttattgtt atcattggct
    13621 taattgtcgt cgccgcatcg tttcgtttta tgcaacagcg acgggaaaaa gctgataatg
    13681 atatggctcc gctccagcaa aagctggtgg tggtgagcaa caagcgggaa aaaccgatta
    13741 acgatcgccg ttcgcgccag caggaagtga ctccggcagg caccagtatg cgctatgagg
    13801 caagcttcaa accgcaaagc ggaggaatgg agcaaacgtt tcgcctcgac gcccagcagt
    13861 accacgccct gacagtgggc gataaaggta cgctgagcta taaaggaacg cgctttgtta
    13921 gctttgttgg cgaacaataa ggttattttt taactttaaa tttcttctgc cagaccagca
    13981 gttcaaacac gccgaaaagg aaaatccgca ccttttcggc ggtggtcatc tgcgggccat
    14041 ctttcggtaa agtggatttc aatagcgcca gctgcatacc atgcatcagc acggtaaaaa
    14101 tcagcgccac gttaacgaaa atattcagcg ggcgcgggaa gggatgcacc aggttgagga
    14161 ttaaaaatcc ccaaacgcag agcattaaca aacgtccaat attaatcagc atcactttct
    14221 ccttgtgctt cgcgttgata cagccgataa gccacctgac ccgccacttt ttcccgatgc
    14281 aatgaccagt ttgccggaac agtgggcaga ccgttttcga cttcgctttc gacataaatc
    14341 agggcttcgt cagccagcca gccgttatct tccagtaaat ttatcgtctc ttctaacaag
    14401 ccacggcgga acggtggatc gacaaacact atattatgcg gtgtgccttt ttgcgccagg
    14461 aatgacatcg cgttgctgtt cactacgcgt gcattgcctg cttttagtgt cgccagattc
    14521 ttaattaact gctgagaaac tgcacgatcc atctcaatca acgtggcctc cgcagcgtag
    14581 cgtgataacg cttccagccc cagcgcgccg cttccggcga agcaatccag acattgggcg
    14641 tcaacaatga ccggagccag ccagttaaat aacgtttcgc gtacgcggtc ggtggtgggg
    14701 cgcagacctg ggctatcagg aaccggtagt ttacggcctc gccactgccc gccaataatg
    14761 cggatttggc cgctgccgga atgattcggt tttttcatga taaattgctc aatccgccag
    14821 ataacacata cttgcaggcg gtgatgtgaa ttaagttaag tgatagacta tttcatcatt
    14881 tttttagctg ctatgtacat agcgttaacg ctgtgccatg aagcaacagc gaggagtgta
    14941 gtcgcaaatg gcgaaagaaa aaaaacgtgg ctttttttcc tggctgggct ttggtcaaaa
    15001 agagcagacc ccggaaaaag agacagaagt tcagaatgaa caatcggttg tagaagaaat
    15061 cgttcaggtg caagagcctg cgaaggcttc tgaacaagcc gttgaagagc agccgcaggc
    15121 gcatactgaa gccgaggcgg aaacttttgc tgccgacgtt gtggaagtca ctgaacaggt
    15181 tgctgaaagt gaaaaagcgc agcctgaagc gggagtcgtt gcacagccgg aaccggtcgt
    15241 agaagaaacg ccggagccag tggctatcga acgtgaagag ctgccgttgc cggaagacgt
    15301 caacgccgaa gaggtttcgc cagaagagtg gcaggctgaa gcggaaaccg tagagattgt
    15361 cgaagcggcg gaagaagaag cggctaaaga agaaatcacc gacgaagagc tggaagcaca
    15421 ggcgctggct gccgaagcgg cagaagaggc ggtgatggtg gttcctccgg cagaagaaga
    15481 gcagccggtg gtagaaatcg ctcaggagca ggaaaaaccg accaaagaag gtttcttcgc
    15541 gcgcctgaaa cgcagcctgt taaaaaccaa agaaaatctc ggttccggat ttatcagcct
    15601 gttccgcggt aaaaaaatcg acgatgatct gtttgaagag ctggaagaac agcttttgat
    15661 cgccgatgtg ggcgtggaaa ccacacgtaa aattatcacc aatctgacgg aaggcgcatc
    15721 ccgcaagcag cttcgtgatg ccgaggcgct ctatggcctg ctgaaagaag agatgggcga
    15781 gattctggcg aaagtcgatg agccgctgaa tgttgaaggt aaaacgccgt tcgtgatcct
    15841 gatggtgggc gtcaacggtg tgggtaaaac cacgacgatt ggtaagctgg cacgtcagtt
    15901 tgagcagcag ggtaaatcgg tgatgctggc ggcgggcgat actttccgtg cagcagcggt
    15961 tgaacagctt caggtctggg gtcagcgcaa caatattccg gtgattgccc agcataccgg
    16021 tgcggattcc gcctctgtta tcttcgacgc cattcaggca gctaaagcgc gtaatatcga
    16081 cgtcctgatt gccgataccg ccggacgcct gcagaacaaa tcgcacctga tggaagagtt
    16141 gaagaaaatc gtccgcgtga tgaagaaact cgacgttgaa gcgccgcatg aagttatgct
    16201 gactatcgat gccagcaccg ggcagaacgc ggtaagccag gccaaactgt tccatgaagc
    16261 cgttggttta accggcatca cgctaacgaa actggacggc acggcgaaag gcggggtaat
    16321 tttctcggtg gctgaccagt ttggtatccc tatccgctac attggtgtcg gcgaacgtat
    16381 tgaggatttg cgtccgttta aggcggacga ctttatagag gcactttttg cccgagagga
    16441 ttaacaatga ttcgctttga acatgtcagc aaggcttatc tcggtgggag acaggcgctg
    16501 cagggcgtta cgttccatat gcagccgggt gagatggcgt ttctgaccgg tcattccggc
    16561 gcagggaaaa gtaccctcct gaagctgatc tgtgggattg agcggcccag cgccgggaaa
    16621 atctggttta gcggccatga catcacgcgt ctgaaaaacc gtgaagttcc gtttctgcgc
    16681 cgccagattg gcatgatttt ccaggatcac catctactga tggaccgtac tgtctacgat
    16741 aacgtggcga tcccgctgat tatcgctggt gccagcggtg acgatattcg tcgccgggtg
    16801 tcggcggcac tggataaagt cgggctactg gacaaagcga agaacttccc tattcagctt
    16861 tcgggcggtg aacaacagcg tgttggcatt gcccgcgcgg tggtgaataa gcccgcggta
    16921 ctgctggcgg acgaaccgac tggtaacctg gacgacgcgt tgtcggaagg gattctacgt
    16981 ctgtttgaag agtttaaccg cgttggggta accgtattga tggcaacgca cgacctcaac
    17041 ctgatttcac ggcgttccta tcgcatgctc accctgagcg atggtcactt gcatggaggc
    17101 gtgggccatg aataagcgcg atgcaatcaa tcatattcgg cagtttggcg ggcgtctgga
    17161 tcgcttccgt aaatcggtcg gcggctcagg cgatggcggt cgtaacgcac cgaaacgcgc
    17221 gaaatcctcg ccaaaaccgg taaatcgcaa aaccaacgtt ttcaacgaac aggtgcgcta
    17281 tgccttccac ggcgcattgc aggatctgaa aagcaaaccg ttcgccacat ttttaacggt
    17341 gatggttatc gccatctccc tgacgctgcc cagcgtctgt tatatggtgt acaaaaacgt
    17401 taaccaggcg gcgacgcagt attatccgtc accgcaaatc actgtttatc tgcaaaaaac
    17461 gctggacgat gacgctgctg cgggcgtggt ggcacagttg caggccgagc aaggcgtgga
    17521 gaaagtgaat tatctttctc gtgaagacgc gctgggcgag ttccgtaact ggtctggctt
    17581 tggcggcgcg ctggatatgc tggaagaaaa cccgcttccg gcagtggcgg tggtgatccc
    17641 gaaactcgat tttcagggga cggaatcact caatacgctg cgtgatcgta tcacgcagat
    17701 taacggcatt gacgaagtgc ggatggatga cagctggttt gcccgtctgg cggcgttgac
    17761 tgggctggtc gggcgcgttt cggcgatgat cggcgtattg atggtggcgg ccgtgttcct
    17821 cgtcatcggc aacagcgtac gtctgagcat ctttgctcgc cgtgactcca ttaacgtgca
    17881 gaaactgatt ggtgcgacgg atggtttcat cctgcgcccg ttcctgtacg gtggcgcact
    17941 gctgggattt tctggcgcat tgttgtcact aattttgtca gaaattctgg tactgcgatt
    18001 gtcatcggcg gttgcggaag tggcacaggt tttcggaacg aagtttaata tcaatggttt
    18061 atcgttcgat gaatgcctgc tgttgctgct ggtatgctcg atgattggct gggtggcagc
    18121 gtggcttgcc acggtacaac atttacgcca ctttacgcct gaataataaa agcgtgttat
    18181 actctttccc tgcaatgggt tccgtagcag ggaaagagac cccgttgtct cttcccggta
    18241 tttcatctct atgtcacatt ttgtgcgtaa tttattcaca agcttgcatt gaacttgtgg
    18301 ataaaatcac ggtctgataa aacagtgaat gataacctcg ttgctcttaa gctctggcac
    18361 agttgttgct accactgaag cgccagaaga tatcgattga gaggatttga atgactgaca
    18421 aaatgcaaag tttagcttta gccccagttg gcaacctgga ttcctacatc cgggcagcta
    18481 acgcgtggcc gatgttgtcg gctgacgagg agcgggcgct ggctgaaaag ctgcattacc
    18541 atggcgatct ggaagcagct aaaacgctga tcctgtctca cctgcggttt gttgttcata
    18601 ttgctcgtaa ttatgcgggc tatggcctgc cacaggcgga tttgattcag gaaggtaaca
    18661 tcggcctgat gaaagcagtg cgccgtttta acccggaagt gggtgtgcgc ctggtctcct
    18721 tcgccgttca ctggatcaaa gcagagatcc acgaatacgt cctgcgtaac tggcgtatcg
    18781 tcaaagttgc gaccaccaaa gcgcagcgca aactgttctt caacctgcgt aaaaccaagc
    18841 agcgtctggg ctggtttaac caggatgaag tcgaaatggt ggcccgtgaa ctgggcgtaa
    18901 ccagcaaaga cgtacgtgag atggaatcac gtatggcggc acaggacatg acctttgacc
    18961 tgtcttccga cgacgattcc gacagccaac cgatggcacc ggtgctctat ctgcaggata
    19021 aatcatctaa ctttgccgac ggcatcgaag atgataactg ggaagagcag gcggcaaacc
    19081 gtctgaccga cgcgatgcag ggtctggacg aacgcagcca ggacatcatc cgcgcgcgct
    19141 ggctggacga agacaacaag tccacgttgc aggaactggc tgaccgttac ggcgtttccg
    19201 ctgaacgtgt acgccagctg gaaaagaacg cgatgaaaaa actgcgcgct gctatagaag
    19261 cgtaatttcc gctattaagc agagaaccct ggatgcgagt ccggggtttt tgttttttga
    19321 gcctctacaa taatcaattt cccctccggc aaaacgccaa tccccacgca gattgttaat
    19381 aaactgtcaa aatagctatt ccaatatcat aaaaatcggg tatgttttag cagagtatgc
    19441 tgctaaagca cgggtagtca tgcataaaac gaaataaagt gctgaacaac aacatcacaa
    19501 cacacgtaat aaccagaaga atggggattc tcaggatgaa cataaagggt aaagcgttac
    19561 tggcaggatg tatcgcgctg gcattcagca atatggctct ggcagaagat attaaagtcg
    19621 cggtcgtggg cgcaatgtcc ggtccggtgg cccagtacgg tgaccaggag tttaccggcg
    19681 cagagcaggc ggttgcggat atcaacgcta aaggcggcat taaaggcaac aaactgcaaa
    19741 tcgtaaaata tgacgatgcc tgtgacccga aacaggcggt tgcggtggcg aacaaagtcg
    19801 ttaacgacgg cattaaatac gtgattggtc acctctgttc ttcatcaacg cagcctgcgt
    19861 ctgatatcta cgaagacgaa ggcattttaa tgatcacccc agcggcaacc gcgccggagc
    19921 tgaccgcccg tagctatcag ctgatcctgc gcaccaccgg cctggactcc gaccaggggc
    19981 cgacggcggc gaaatatatt cttgagaaag tgaaaccgca gcgtattgct atcgttcacg
    20041 acaaacagca atacggcgaa ggtctggcgc gagcggtgca ggacggcctg aagaaaggca
    20101 atgctaacgt ggtgttcttt gatggtatca ccgccgggga aaaagatttc tcaacgctgg
    20161 tggcgcgtct gaaaaaagag aatatcgact tcgtttacta cggcggttat cacccggaaa
    20221 tggggcaaat cctgcgtcag gcacgcgcgg cagggctgaa aactcagttt atggggccgg
    20281 aaggtgtggc taacgtttcg ctgtctaaca ttgcgggcga atcagcggaa gggctgctgg
    20341 tgaccaagcc gaagaactac gatcaggttc cggcgaacaa acccattgtt gacgcgatca
    20401 aagcgaaaaa acaggaccca agcggcgcat tcgtctggac cacctacgcc gcgctgcaat
    20461 ctttgcaggc gggcctgaat cagtctgacg atccggctga aatcgccaaa tacctgaaag
    20521 cgaactccgt ggataccgta atgggaccgc tgacgtggga tgagaaaggc gatctgaaag
    20581 gctttgagtt cggcgtattt gactggcacg ccaacggcac ggcgaccgat gcgaagtaat
    20641 cattaatcgg caactttggg ttgccgccaa attgctaata tcgagtacgt tgcttcatgc
    20701 cggatgcggc gtaaacgcct tatccggcct acaagatcca aagaaatcag taaattgcaa
    20761 cacacattgt aggcctgata agcgtagcgc atcaggcaat ttctcgttgg tcatcacttt
    20821 tgaaatcaac ttgacgatta acgcttctcc cagccgccct gttgcgccgt aaatcccagt
    20881 gcctgcataa acgccgtcat cacaccgcgt tcttccacgc ctgcatccgc catccaccag
    20941 catgaaacgc caggattgtt acgcaaaacc tcttccagca gatattgccc cacaccgcga
    21001 cgtcgggtga cttcccgcac acgcagggaa tccagtgctc cctcggtgcc gcttaaggtt
    21061 acccgtacgg cagcgagcag gcgctcgtta aaacgcgcgg cgtagatacg gtggttatcg
    21121 tcaacctgta aagaggaagg ggaatactcc ggccagatct tttgcaggtc aatccggtcc
    21181 tggtcgctaa agttttctaa tcgaatgatg gtcagcttca tgggtaaccc gtgtaaatca
    21241 caaaagtgga accagtgtag cgaaataatt taatcggagg ctttctcttt tttatttctt
    21301 ttggcaggtg attaattttt taacagcaat aattacaaag ttaaaacatt agagaatgaa
    21361 aaataccca
//
LOCUS       DAPHBJ010000068        20938 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.068, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000068 DAPHBJ010000000
VERSION     DAPHBJ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.068"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1146
                     /gene="proV"
                     /locus_tag="RHZ76_002243"
     CDS             <1..1146
                     /gene="proV"
                     /locus_tag="RHZ76_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HDV2812883.1"
                     /translation="RAFKYIEQGLSKEQILEKTGLSLGVKDASLAIEEGEIFVIMGLS
                     GSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMVFQSFALMPHM
                     TVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHSYPDELSGGMRQRVGLARAL
                     AINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGDRIAI
                     MQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFG
                     PRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALTQQQGLDAALIDAPLAVDAQ
                     TPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
     gene            1139..2203
                     /gene="proW"
                     /locus_tag="RHZ76_002244"
     CDS             1139..2203
                     /gene="proW"
                     /locus_tag="RHZ76_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HDV2812884.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2261..3253
                     /gene="proX"
                     /locus_tag="RHZ76_002245"
     CDS             2261..3253
                     /gene="proX"
                     /locus_tag="RHZ76_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HDV2812885.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTHTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            3545..4729
                     /locus_tag="RHZ76_002246"
     CDS             3545..4729
                     /locus_tag="RHZ76_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2812886.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALSGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            4853..5590
                     /gene="ygaZ"
                     /locus_tag="RHZ76_002247"
     CDS             4853..5590
                     /gene="ygaZ"
                     /locus_tag="RHZ76_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HDV2812887.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWIAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            5580..5915
                     /gene="ygaH"
                     /locus_tag="RHZ76_002248"
     CDS             5580..5915
                     /gene="ygaH"
                     /locus_tag="RHZ76_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HDV2812888.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            6006..6536
                     /gene="emrR"
                     /locus_tag="RHZ76_002249"
     CDS             6006..6536
                     /gene="emrR"
                     /locus_tag="RHZ76_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HDV2812889.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            6663..7835
                     /gene="emrA"
                     /locus_tag="RHZ76_002250"
     CDS             6663..7835
                     /gene="emrA"
                     /locus_tag="RHZ76_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HDV2812890.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            7852..9390
                     /gene="emrB"
                     /locus_tag="RHZ76_002251"
     CDS             7852..9390
                     /gene="emrB"
                     /locus_tag="RHZ76_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HDV2812891.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(9454..9969)
                     /gene="luxS"
                     /locus_tag="RHZ76_002252"
     CDS             complement(9454..9969)
                     /gene="luxS"
                     /locus_tag="RHZ76_002252"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDV2812892.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(10119..11675)
                     /gene="gshA"
                     /locus_tag="RHZ76_002253"
     CDS             complement(10119..11675)
                     /gene="gshA"
                     /locus_tag="RHZ76_002253"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDV2812893.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(11748..12176)
                     /locus_tag="RHZ76_002254"
     CDS             complement(11748..12176)
                     /locus_tag="RHZ76_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV2812894.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12173..12739)
                     /gene="yqaB"
                     /locus_tag="RHZ76_002255"
     CDS             complement(12173..12739)
                     /gene="yqaB"
                     /locus_tag="RHZ76_002255"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HDV2812895.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(13020..13096)
                     /locus_tag="RHZ76_002256"
     tRNA            complement(13020..13096)
                     /locus_tag="RHZ76_002256"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13060..13062),aa:Arg,seq:acg)
     gene            complement(13295..13371)
                     /locus_tag="RHZ76_002257"
     tRNA            complement(13295..13371)
                     /locus_tag="RHZ76_002257"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13335..13337),aa:Arg,seq:acg)
     gene            complement(13434..13510)
                     /locus_tag="RHZ76_002258"
     tRNA            complement(13434..13510)
                     /locus_tag="RHZ76_002258"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13474..13476),aa:Arg,seq:acg)
     gene            complement(13709..13785)
                     /locus_tag="RHZ76_002259"
     tRNA            complement(13709..13785)
                     /locus_tag="RHZ76_002259"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13749..13751),aa:Arg,seq:acg)
     gene            complement(13789..13881)
                     /locus_tag="RHZ76_002260"
     tRNA            complement(13789..13881)
                     /locus_tag="RHZ76_002260"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13845..13847),aa:Ser,seq:gct)
     gene            complement(14197..14382)
                     /gene="csrA"
                     /locus_tag="RHZ76_002261"
     CDS             complement(14197..14382)
                     /gene="csrA"
                     /locus_tag="RHZ76_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDV2812896.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(14617..17247)
                     /gene="alaS"
                     /locus_tag="RHZ76_002262"
     CDS             complement(14617..17247)
                     /gene="alaS"
                     /locus_tag="RHZ76_002262"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDV2812897.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNTQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVVEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(17375..17875)
                     /gene="recX"
                     /locus_tag="RHZ76_002263"
     CDS             complement(17375..17875)
                     /gene="recX"
                     /locus_tag="RHZ76_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDV2812898.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQEI
                     WRNFAD"
     gene            complement(17944..19005)
                     /gene="recA"
                     /locus_tag="RHZ76_002264"
     CDS             complement(17944..19005)
                     /gene="recA"
                     /locus_tag="RHZ76_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDV2812899.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(19085..19582)
                     /gene="pncC"
                     /locus_tag="RHZ76_002265"
     CDS             complement(19085..19582)
                     /gene="pncC"
                     /locus_tag="RHZ76_002265"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HDV2812900.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(19727..20812)
                     /gene="mltB"
                     /locus_tag="RHZ76_002266"
     CDS             complement(19727..20812)
                     /gene="mltB"
                     /locus_tag="RHZ76_002266"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HDV2812901.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETDTTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
BASE COUNT         4818 a         5655 c         5289 g         5176 t
ORIGIN      
        1 cgagcgttca aatatatcga acaaggactt tcaaaagaac aaattctgga aaaaactggg
       61 ctatcgcttg gcgtaaaaga cgccagtctg gccattgaag aaggcgagat atttgtcatc
      121 atgggattat ccggctcggg taaatccaca atggtacgcc ttctcaatcg cctgattgaa
      181 cccacccgcg ggcaagtgct gattgatggt gtggatattg ccaaaatatc cgacgccgaa
      241 ctccgtgagg tgcgcagaaa aaagattgcg atggtcttcc agtcctttgc cttaatgccg
      301 catatgaccg tgctggacaa tactgcgttc ggtatggaat tggccggaat taatgccgaa
      361 gaacgccggg aaaaagccct tgatgcactg cgtcaggtcg ggctggaaaa ttatgcccac
      421 agctacccgg atgaactctc tggcgggatg cgtcaacgtg tgggattagc ccgcgcgtta
      481 gcgattaatc cggatatatt attaatggac gaagccttct cggcgctcga tccattaatt
      541 cgcaccgaga tgcaggatga gctggtaaaa ttacaggcga aacatcagcg caccattgtc
      601 tttatttccc acgatcttga tgaagccatg cgtattggcg accgaattgc cattatgcaa
      661 aatggtgaag tggtacaggt cggcacaccg gatgaaattc tcaataatcc ggcgaatgat
      721 tatgtccgta ccttcttccg tggcgttgat attagtcagg tattcagtgc gaaagatatt
      781 gcccgccgga caccgaatgg cttaattcgt aaaacccctg gcttcggccc acgttcggca
      841 ctgaaattat tgcaggatga agatcgcgaa tatggctacg ttatcgaacg cggtaataag
      901 tttgtcggcg cagtctccat cgattcgctt aaaaccgcgt taacgcagca gcaaggtctt
      961 gatgcggcgc tgattgatgc gccgttagca gtcgatgcac aaacgcctct tagcgagttg
     1021 ctctctcatg tcggacaggc accctgtgcg gtgcccgtgg tcgacgagga ccaacagtat
     1081 gtcggcatca tttcgaaagg aatgctgctg cgcgctttag atcgtgaggg ggtaaataat
     1141 ggctgatcaa aataatccgt gggataccac gccagcagcg gacagtgccg cgcaatccgc
     1201 agacgcctgg ggtacaccga cgactgcacc gactgacggc ggtggtgctg actggctgac
     1261 cagtacgcct gcgccaaacg tcgagcattt taatattctc gatccgttcc ataaaacgct
     1321 gatcccgctc gacagttggg tcactgaagg gatcgactgg gtcgttaccc atttccgtcc
     1381 cgtcttccag ggcgtgcgcg ttccggttga ttatatcctc aacggtttcc agcaattgct
     1441 gctgggtatg cccgcaccgg tggcgattat cgttttcgct ctcatcgcct ggcagatttc
     1501 cggggtcgga atgggtgtgg cgacgctggt ttcgctgatt gccatcggcg caatcggtgc
     1561 ctggtcgcag gcaatggtga ctctggcgct ggtgttaacc gccctgctgt tctgtatcgt
     1621 catcggtttg ccgttgggga tatggctggc gagaagtccg cgagcggcga aaattattcg
     1681 tccactgctt gatgccatgc agaccacgcc agcgtttgtt tatctggtgc caatcgtcat
     1741 gctatttggt atcggtaacg tgccgggcgt ggtggtgacg atcatctttg ctctgccgcc
     1801 gattatccgt ctgaccattc tggggattaa ccaggttccg gcggatctga ttgaagcctc
     1861 gcgctcattc ggtgccagcc cgcgccagat gctgttcaaa gttcagttac cgctggcgat
     1921 gccgaccatt atggcgggcg ttaaccagac gctgatgctg gccctttcta tggtggtcat
     1981 cgcctcgatg attgccgtcg gcgggttggg tcagatggta cttcgcggta tcggtcgtct
     2041 ggatatgggg cttgccaccg ttggcggcgt cgggattgtg atcctcgcca ttatcctcga
     2101 tcgtctgacg caggccgttg ggcgcgactc acgcagtcgc ggcaaccgtc gctggtacac
     2161 cactggccct gttggtctgc tgacccgccc attcattaag taactctgca cttgcccggt
     2221 gacgccgggc attatcaccc tgccaaaaaa aggaataaca atgcgacata gcgtactttt
     2281 tgcgacagcg tttgccacgc ttatctctac acaaactttt gctgccgatc tgccgggcaa
     2341 aggcattact gttaatccag ttcagagcac catcactgaa gaaaccttcc agacgctgct
     2401 ggtcagtcgt gcgctggaga aattaggtta taccgtcaac aaacccagcg aagtagatta
     2461 caacgttggc tacacctcgc ttgcttccgg cgatgcaacc ttcaccgccg tgaactggac
     2521 gccactgcat gacaacatgt acgaagctgc cggtggcgat aagaaatttt atcgtgaagg
     2581 ggtatttgtt aacggcgcgg cacagggtta cctgatcgat aagaaaaccg ccgaccagta
     2641 caaaatcacc aacatcgcac aactgaaaga tccgaagatc gccaaactgt tcgataccaa
     2701 cggcgacgga aaagcggatt taaccggttg taaccctggc tggggctgcg aaggtgcgat
     2761 caaccaccag cttgccgcgt atgaactaac ccataccgtg acgcataatc aggggaacta
     2821 cgcggcgatg atggccgaca ccatcagtcg ctacaaagag ggcaaaccgg tgttttacta
     2881 cacctggacg ccgtactggg tgagtaatga gctgaagcca gggaaagatg tggtctggtt
     2941 gcaggtgccg ttctccgcac tgccgggcga taaaaacgcc gataccaaac tgccgaatgg
     3001 tgcgaattat ggcttcccgg tcagcaccat gcatatcgtt gccaacaaag cctgggccga
     3061 gaaaaacccg gcagcagcga aactgtttgc cattatgcag ttgccagtgg cagatattaa
     3121 cgcccagaac gccattatgc atgacggcaa agcctcagaa ggcgatattc agggccatgt
     3181 tgatggctgg atcaaagccc accagcagca gttcgatggc tgggtgaatg aggcgctggc
     3241 agcgcagaag taatttttat tcgggcggat aaggcgtttt tacctcatcc gccgctctgt
     3301 acacaatgcc tgatgcgacg ctggagcgtc ttatcatgcc tacaaaatac actcattccg
     3361 tatggcggat aaggcgtttt cgccgcatcc gccgttctgt gcacaatgcc tgatgcgacg
     3421 ctggcgcgtc ttatcatgcc tacaaaccac atcgcacatt tcaacaagct atctttcatc
     3481 ccatattcat caacatccgc tattattgat ttccagctta atcatcacct gatgaacaaa
     3541 aataatgact aaacctaatc atgagcttag cccggcgctg atcgtgctga tgtctatcgc
     3601 caccggtctg gcggtcgcca gtaactatta cgctcagcca ttgctcgaca ccatcgcgcg
     3661 taacttttcc ctttccgcca gttcggcagg ctttattgtt accgccgcgc aattgggcta
     3721 tgcagcaggt ctgctgtttc ttgttcccct cggtgatatg tttgaacgcc gccgcctgat
     3781 tgtctcgatg accttactgg cggcaggcgg catgttaatc actgccagca gtcagtcgct
     3841 ggcgatgatg atcctcggta cggcattaag cggtttattc tctgtcgtgg cacaaattct
     3901 ggttccgctg gcagcgacgc tggcttcacc ggacaaacgc ggcaaagtgg ttggcactat
     3961 tatgagcggt ctgctgttgg ggatcttgct ggcacggaca gttgccggat tgctggcgaa
     4021 tctcggcggc tggcgcaccg tcttttgggt tgcttcggtg ttaatggcac tgatggcgct
     4081 ggcattatgg cgtggtctgc cacaaatgaa atcagaaacc cacctcaact acccacagtt
     4141 gttgggttcc gttttcagta tgtttatcag cgataaaatt ctgcgtaccc gcgcgttgct
     4201 gggctgcctg acctttgcca atttcagcat tctctggacc tcaatggcct ttttgcttgc
     4261 cgctccacct tttaactaca gcgatggtgt aattggtctg tttggacttg cgggagctgc
     4321 cggagcgttg ggcgctcgtc cggcgggcgg ttttgccgat aagggcaaat cgcaccacac
     4381 cacaactttc ggtctgctgc tgctattact ttcatggctg gcgatctggt ttggtcacac
     4441 ttccgtactg gcgttgatta tcggaatcct ggtgctggat ctcaccgtgc agggcgtgca
     4501 tatcactaac cagacggtaa tttatcggat acatcctgat gcgcgcaatc gcctgaccgc
     4561 aggttacatg actagctact ttattggcgg tgccgccggt tcgctaattt cagcctcagc
     4621 ctggcaacat ggcggttggg ctggcgtttg tctggctggc gcgacgattg ccctggttaa
     4681 cttactggtc tggtggcgag gttttcatcg tcaggaagcc gcaaattaag caatcagcaa
     4741 ccgttttcgt ggctttacac ttataagggt gttaagaagc ccatcagtct gataaggtta
     4801 agatattcat tcagtctatt tataatatta acaatcgtta agcgtacact ctatggaaag
     4861 ccctactcca cagcctgctc ctggttcggc gaccttcatg gaaggatgca aagacagttt
     4921 accgattgtt attagttata ttccggtggc ctttgcgttc ggtctgaatg cgacccgtct
     4981 gggattctct cctctcgaaa gcgttttttt ctcctgcatc atttatgcag gcgcgagcca
     5041 gttcgtcatt accgcgatgc tggcagccgg gagtagtttg tggattgctg cactgaccgt
     5101 catggcaatg gatgttcgcc atgtgttgta tggcccgtca ctgcgtagcc gtattattca
     5161 gcgtctgcaa aaatcgaaaa ccgccctgtg ggcgtttggc ctgacggatg aggtttttgc
     5221 cgccgcaacc gcaaaactgg tacgcaataa tcgccgctgg agcgagaact ggatgatcgg
     5281 cattgccttc agttcatggt catcgtgggt atttggtacg gtaatagggg cattctccgg
     5341 cagcggcttg ctgcaaggtt atcccgccgt tgaagctgca ttaggtttta tgcttccggc
     5401 actctttatg agtttcctgc tcgcctcttt ccagcgcaaa caatctcttt gcgttaccgc
     5461 agcgttagtt ggtgcccttg caggcgtaac gctattttct attcccgtcg ccattctggc
     5521 aggcattgtc tgtggctgcc tcactgcgtt aatccaggca ttctggcaag gagcgcccga
     5581 tgagctatga ggttctgctg cttgggttac tagttggcgt ggcgaattat tgcttccgct
     5641 atttgccgct gcgcctgcgt gtgggtaatg cccgcccaac caaacgtggc gcggtaggta
     5701 ttttgctcga caccattggc atcgcctcga tatgcgctct gctggttgtc tctaccgcac
     5761 cagaagtgat gcacgataca cgccgtttcg tgcccacgct ggtcggcttc gcggtactgg
     5821 gtgccagttt ctataaaaca cgcagcatta tcatcccaac actgcttagt gcgctggcct
     5881 atgggctcgc ctggaaagtg atggcgatta tataacccac aagaatcatt tttctaaaac
     5941 aatacattta ctttatttgt cactgtcgtt actatatcgg ctgaaattaa tgaggtcata
     6001 cccaaatgga tagttcgttt acgcccattg aacaaatgct aaaatttcgc gccagccgcc
     6061 acgaagattt tccttatcag gagatccttc tgactcgtct ttgcatgcac atgcaaagca
     6121 agctgctgga gaaccgcaat aaaatgctga aggctcaggg gattaacgag acgttgttta
     6181 tggcgttgat tacgctggag tctcaggaaa accacagtat tcagccttct gaattaagtt
     6241 gtgctcttgg atcatcccgt accaacgcga cgcgtattgc cgatgaactg gaaaaacgcg
     6301 gttggatcga acgtcgtgaa agcgataacg atcgccgctg cctgcatctg caattaacgg
     6361 aaaaaggtca cgagtttttg cgcgaggttt taccaccgca gcataactgc ctgcatcaac
     6421 tctggtccgc gctcagcaca acagaaaaag atcagctcga gcaaatcacc cgcaaattgc
     6481 tctcccgtct cgaccagatg gaacaagacg gtgtggttct cgaagcgatg agctaacgcg
     6541 tcatctcgct caaaaatcca gatttataaa agaaaaaatg actggccagc atcgcaacat
     6601 gctggccttt ttggcaagca ggtcggctca gccgatgagt taagaagatc gtggagaaca
     6661 atatgagcgc aaatgcggag actcaaaccc cgcagcaacc ggtaaagaag agcggcaaac
     6721 gtaagcgtct gctcctcctt ctcaccttgc tctttataat tattgccgta gcgataggga
     6781 tttattggtt tttggtactg cgtcacttcg aagaaaccga tgacgcatac gtggcaggga
     6841 atcaaattca aattatgtct caggtgtctg gcagcgtgac gaaagtctgg gccgataaca
     6901 ccgattttgt aaaagaaggc gacgtgctgg tcactctcga cccgacagat gctcgccagg
     6961 cgtttgaaaa agccaaaact gcactggctt ccagcgttcg ccaaacccac cagctgatga
     7021 ttaacagcaa gcagttgcag gcgaatattg aggtgcagaa aatcgccctc gcgaaagcac
     7081 aaagcgacta caaccgccgt gtgccgctgg gcaatgccaa cctgattggt cgcgaagagc
     7141 tgcaacacgc ccgcgacgcc gtcaccagtg cccaggcgca actggacgtc gcgattcaac
     7201 aatacaatgc caatcaggcg atgattctgg ggactaaact ggaagatcag ccagccgtgc
     7261 aacaggctgc caccgaagta cgtaacgcct ggctggcgct ggagcgtact cgtattgtca
     7321 gtccgatgac cggttatgtc tcccgccgcg cggtacagcc tggggcgcaa attagcccaa
     7381 cgacgccgct gatggcggtc gttccagcca ccaatatgtg ggtggatgcc aactttaaag
     7441 agacgcagat tgccaatatg cgtatcggtc agccggtcac tatcaccacg gatatttacg
     7501 gcgatgatgt gaaatacacc ggtaaagtgg ttggtctgga tatgggcaca ggtagcgcgt
     7561 tctcactgct tccagcgcaa aatgcgaccg gtaactggat caaagtcgtt cagcgtctgc
     7621 ctgtgcgtat cgaactggac cagaaacagc tggagcaata tccgctgcgt atcggtttgt
     7681 ccacgctggt gagcgtcaat accactaacc gtgacggtca ggtactggca aataaagtac
     7741 gttccactcc ggtagcggta agcaccgcgc gtgaaatcag cctggcacct gtcaataaac
     7801 tgatcgacga tatcgtaaaa gctaacgctg gctaatccag aggtgcgtgt gatgcaacag
     7861 caaaaaccgc tggaaggcgc gcaactggtc attatgacga ttgcgctgtc actggcgaca
     7921 ttcatgcagg tgctggactc caccattgct aacgtggcga tccccactat cgccgggaat
     7981 ctgggctcat cgctcagcca gggaacgtgg gtaatcactt ctttcggggt ggcgaatgcc
     8041 atctcgatcc cgcttaccgg ctggctggca aagcgcgtcg gggaagtgaa actgttcctt
     8101 tggtccacca tcgcctttgc tattgcgtcg tgggcgtgtg gtgtctccag cagcctgaat
     8161 atgctgatct tcttccgcgt gattcagggg attgtcgccg ggccgttgat cccgctttcg
     8221 caaagtctat tgctgaataa ctacccgcca gccaaacgct cgatcgcgct ggcgttgtgg
     8281 tcgatgacgg tgattgtcgc gccaatttgc ggcccgatcc tcggcggtta tatcagcgat
     8341 aattaccact ggggctggat attcttcatc aacgtgccga ttggcgtggc ggtggtgttg
     8401 atgacactgc aaactctgcg cggacgtgaa acccgcaccg aacggcggcg gattgatgcc
     8461 gtggggctgg cactgctggt tattggtatc ggcagcctgc agattatgct cgaccgcggt
     8521 aaagagctgg actggttttc atcacaggaa attatcatcc ttaccgtggt ggcggtggtg
     8581 gctatctgct tcctgattgt ctgggagctg accgacgata acccgatagt cgatctgtcg
     8641 ttgtttaagt cgcgcaactt caccatcggc tgcttgtgta tcagcctcgc gtatatgctc
     8701 tacttcggcg ctattgttct gctgccgcag ttgttgcagg aggtctacgg ttacacggcg
     8761 acctgggcag gtttggcctc tgcgccggta gggattattc cggtgatcct gtcgccgatt
     8821 atcggccgct tcgcgcataa actggatatg cggcggctgg taaccttcag ctttattatg
     8881 tatgccgtct gcttctactg gcgtgcctat acctttgaac caggtatgga ttttggcgcg
     8941 tcggcctggc cgcagtttat ccaggggttt gcggtggcct gcttctttat gccgctgacc
     9001 accattacgc tgtctggttt gccaccggaa cgactggcgg cggcatcgag cctctctaac
     9061 tttacgcgaa cgctggcggg gtctatcggc acgtcgataa ccacgaccat gtggaccaac
     9121 cgcgagtcga tgcaccatgc gcagttgact gagtcggtaa acccgttcaa cccgaatgcc
     9181 caggcgatgt acagtcaact ggaagggctt gggatgacgc aacagcaggc atcaggctgg
     9241 attgcccagc agatcaccaa tcaggggctg attatttccg ccaatgagat cttctggatg
     9301 tcagccggga tattcctcgt cctgctgggg ctggtgtggt ttgctaaacc gccatttggc
     9361 gcaggtggcg gcggaggcgg tgcgcactaa gtacaactaa gccagttcat ttgaactggc
     9421 ttttttcaat taattgtgaa gatagtttac tgactagatg tgcagttcct gcaacttctc
     9481 tttcggcagt gccagttctt cgttgctgtt gatgcgtacg tcacgttcca gaatgctacg
     9541 cgcaatatcc tgcgcttcct gcaacgagtg catctggtaa gtgccacact ggtagacgtt
     9601 cagttccggg atctgattct gatcctgcac tttcagcacg tcttccattg ccgctttcca
     9661 ggcatcagca acacgctgct catctggcgt accaatcaga ctcatataaa aaccggtgcg
     9721 gcagcccatt ggcgagatat cgataatctc tacaccatta ccgttaagat ggttacgcat
     9781 aaaaccagca aacaggtgct ccagggtatg gatccctctt tctggcatca cttctttgtt
     9841 cggcacgcag aagcgcagat cgaacacggt gattgcgtcg ccatgcgggg tgttcattgt
     9901 tttcgccacc cgaactgcag gcgcttccat ccgggtatga tcgactgtga agctatctaa
     9961 caacggcatt tagccacctc cggtaatttt tttaaaaatt ttctgaactc tttcttccca
    10021 ggcgagtctg agtatatgaa agacgcgcat ttgttatcat catccctgaa ttcagagatg
    10081 aaattttggc cactcacgag tggccttttt cttttctgtc aggcgtgttt ttccagccac
    10141 accgcaaacg gttcggtatc agcggcttcc atttcctgct gacggcgttc agacgcctcg
    10201 cgctcggcta caaaatcctc ttcgcgcaga atttccagcg gctcttcacg cagcagatta
    10261 cggtaggctt ctgcaaatgc tttgcctgtt ccgccaatac cagtatcaat catagacctt
    10321 aagatacggg cagagaaagt cagatcggga ttatcgaagc aggcaaccag ttcatcacac
    10381 actttctgat acgcttcgcc gccgttaata ctatccagcg tttgcgcgac gcgtttcaga
    10441 tcgcggaaca gatctttacc cacctgcggt aacgggaact gtgcggtttc gcagccgata
    10501 cccagcgtca gacccggttt gcgaccttcg aggatcaccc ggttccagtt aacgcgtgta
    10561 caggcaagtt cgctactgct catttccggt gcatcagcca gcgcacacca gaccataaac
    10621 aggtcgagga atcgcacctg ctgttcatct acaccaatcg gcgagaacgg gttgatgtcc
    10681 agcgaacgca cttcaatata ttcaatgccg ccacgtaaca gcgcatcaga aggcgactcg
    10741 ccgctgcggg taacgcgttt tggacgaatc ggcgcgtaca gttcgttttc aatctgcaac
    10801 acgttgctgt tgatttgcag cctcttaccg tctttctcaa taccaatctt cgcgtactct
    10861 tccgatggcg ttttgattgc ctgtttaagg cccgctacgt actcgtaaag atcgttgaag
    10921 gtaataccaa gattgctttg cgatttattg gtatagccga gatcgctcaa acgaagagag
    10981 gtcgcatacg gcaggtaata cataccgcac tcggttttct caaacggcag cgacgttggt
    11041 tttccttgca ggaaagaaga acaaatcgcc ggagatgcac caaacagata aggaatgacc
    11101 caaccgaaac gatagtaatt gcggataacg cggaaatagc ccgcagaaat tttctctttg
    11161 gcatcagcgc ccgagatatc accgcacttc gcttgccaga atgccattgg caaagagaaa
    11221 ttgtagtgca cgccggaaat ggtttgcatc agcgcgccgt agcgattttt cagcccttca
    11281 cgatacagcg ttttaaagcg tccggtgtta gaagtgccgt actgtgccag ttcgatgtcc
    11341 tgaccttctg cgatgtagca tggcatactt aacggccaca tccgctcatc gcccatattg
    11401 cgcgccgtat aacgatgcag atcgcgcata aaggtcagca tatgttcaat atcaccatcc
    11461 actggtgtaa tgaattccag caatgcttcc gcaaaatcgg tagtaatcca tttgtgcgtc
    11521 agtgcggaac ctaatgcttc aggatgacct gttgttgcca gtgtgccatc agcattaaca
    11581 cgcaaagttt cgcgctccag cccacgctgt atccccttta acgcctgagg atgtttttcc
    11641 agccaggcca gcgcctgtga tacgtccggg atcaaattga cctcccgcct gtcaaaatcg
    11701 ttttaattag cataactgta atggtgacca tatgtgcagg cctacaatta gtgccaccac
    11761 atcatgccct gaacggtcgc tgctgcaact gcaacatagc gtaacgcttt accaaggcac
    11821 aaaaaaaaga ttaccggtcc ccacgagatg cgcatccatc ccgctaacag acacagtaaa
    11881 tcgccaacca cgggcatcca gcttaataat agcgtgactg caccatagcg tttcagccag
    11941 ccggtagctt tctcttgcca gcgcgatgtt ttacgcaatg gaaagaaacg cccaaggata
    12001 acgttagtta accctccaag gctattaccc attgttgctg ttaaaactaa aacccaggga
    12061 tgactgatcc cggaaagcaa cattgccacc agcacgactt cggagttgcc gggtaatagt
    12121 gtagcgctga gaaaactact ggcaaacaac gagaaaagcg ataacgcttc actcacagca
    12181 agcgaacatc cacggcgtcc atgcctgctg cacgggccgc ctgaataccg aaatcggcat
    12241 cttcaaagac cacacactgc gtcggttgca cgcccatacg ctgcgcgcac aacaaaaatg
    12301 tgtctggcgc gggtttatgg tgtttgacgt gatcggcagc gacgacggcg tcaaaataat
    12361 ggcgtaatcc caggtgcgcc agcaatgcct cagcgatggc gctttcactc cccgttccta
    12421 cagccattgg gcgacgacca tgccaacttt tcaccacatc aacaagagga agcggttcga
    12481 cgctatccag cagcatactt cttactgctt ctgttttttc acgcgctaac gcatgcgggt
    12541 cgagatcggc ctgattcagc tcaataattg cctgagcaat acgccaggtg ggcgatccat
    12601 taagcgcaat catcgcctga atatcgtact gaagaccgta gtgccctaat acttcgcgcc
    12661 acgctttacg gtgcgtaggc tccgtatcca ggattgtgcc atccatatca aaaattaaac
    12721 ctgcataacg ctcgtacatg gtcttctcgc aagtcgaaaa atatgacgct actttagcgt
    12781 aattgcttga ttttgtcgct gatggcgaag gagaagaaaa ttcaggaaat ggataaagga
    12841 gttatttaga aatgagatat ttttgaagga aattttttgg agaagatggt gcatccggga
    12901 ggatgactca ctgacgttcg cccttcgggc cgttgctggc gcaacgttat ccttcctggt
    12961 gcctgcggtt aataccgcta tctcttcgat accttcattg ctgaaggtta cggagaatat
    13021 ggtgcatccg ggaggattcg aacctccgac cgctcggttc gtagccgagt actctatcca
    13081 gctgagctac ggatgcatcg ggaaaactta ttctactgct gatattgata ccgctactaa
    13141 agccatatca attaagagat ggtgcatccg ggaggattac tcgccttgcg gctcgccctt
    13201 cgggccgttg ctaaagcaac gttatcatcc ctagtgcctg cggttaattc cgctacctct
    13261 tcgatacctt cattgctgaa gatttcggag aatatggtgc atccgggagg attcgaacct
    13321 ccgaccgctc ggttcgtagc cgagtactct atccagctga gctacggatg catcgggaaa
    13381 tttattttac tgctgatatt gataccgcta ctaaagccat atcaagtaag agatggtgca
    13441 tccgggagga ttcgaacctc cgaccgctcg gttcgtagcc gagtactcta tccagctgag
    13501 ctacggatgc atcgggaaac ttactttact gcagattttt tgataccgct actaaagccg
    13561 tatcaagtaa gagatggtgc atccgggagg attactcgcc ttgcggctcg cccttcgggc
    13621 cgttgctaaa gcaacgttat cctccctggt gcctgcggtt attaccgcca cctcttccat
    13681 accttcatcg ctgaaagtac gtagaatatg gtgcatccgg gaggattcga acctccgacc
    13741 gctcggttcg tagccgagta ctctatccag ctgagctacg gatgcaaatg gcggtgaggc
    13801 ggggattcga accccggatg cagcttttga ccgcatactc ccttagcagg ggagcgcctt
    13861 cagcctctcg gccacctcac cacacgcctc ttacgagtgc ttcgaagaac ttgtttatcg
    13921 ctcatcgtcg ctgcgtggcg cacatattac tttctgagac ttataagtca aacaattttt
    13981 cctacacttt tatcgtttgc acacttcacg ttcaattagt ctgtaaaaac gtcaaaaaga
    14041 gtgttttatc aacagaagaa tggaggtctg acagatagta gtaatgcaaa aaaatggaga
    14101 cttaagttga atgaacggga gtaaagcgaa aagactatag agtgaaggag aaattttgag
    14161 ggtgcgtctc accgataaag atgagacgcg gaaagattag taactggact gctgggattt
    14221 ttcagcctgg atacgctggt agatctcttc acggtgaaca gaaacttcct tcggggcatt
    14281 tacgccaata cgtacctggt tgccctttac ccctaaaact gtcacggtga cctcatcccc
    14341 aatcatgagg gtctcaccaa ctcgacgagt cagaatcagc attctttgct ccttgaaaga
    14401 ttaaaagagt cgggtctctc tgtatcccgg cattatccat catataacgc caaaaagtaa
    14461 gcaatgacaa acacattaca tctaagcagt catggcatta cattctgtta aacctaagtt
    14521 tagccgatat acacaacttc aacctgactt tatcgttgtc gatagcgtta atgcgaatgc
    14581 cgtgaagcga gtccacggca ttgcctgacg cttatattat tgcaatttcg cgctgaccca
    14641 gcctttcaca ctggctaacg ctgcaggtaa ggccgcagca tccgtaccac cggcttgcgc
    14701 catgtcagga cgtccaccac ccttgccgcc cacctgctga gcgaccatac caatcagttc
    14761 ccctgctttc acacgatctg tgacgtcctt agatacgcct gcaatcagag aaaccttacc
    14821 ttcgactacc gttgccagca cgataattgt cgaccccagc tgatttttta aatcgtcaac
    14881 catggtacgc aacattttcg gctcaacacc gctaagctcg ctaaccaaca gcttaacacc
    14941 attaacatca attgccttac tggaaagatt tgcgctctcc tgtgcggcag cttgttcttt
    15001 aagctgttgt aactcttttt ccagctgacg cgtacgttcc agtactgagc gcactttatc
    15061 agccagatta ttgctatcgc ctttcagcag atgcgcgact tcgcttaagc gatcgctgtc
    15121 tgcatgaacg gtggcgatag caccttctcc ggttaccgct tcgatacgac gaacgcctgc
    15181 agcagtaccc gattcagaga tgatgcggaa cagaccaata tcaccagtgc ggctggcgtg
    15241 agtaccgcca cacaactcgg tagagaaatc gcccatgctc agcacgcgta cgcgctcatc
    15301 atacttctcg ccgaacagcg ccatcgcacc tttcgctttc gccgcttcga gatccatgat
    15361 gttggtttcg atcggcaaat tgcgacgaat ctgtgtgttc accaggtctt cgaccgcacg
    15421 aatctcttct ggtttcatcg cttcgttgtg tgagaagtcg aagcgcagca ccttgtcgtt
    15481 aaccagtgaa cctttctgcg atacatgagt acccagaacc tggcgcagcg cagcgtgcat
    15541 caggtgcgtt gcggagtgat tcagacgaat acgggcgcga cgagcctcat caacatcagc
    15601 ctgcaccgcg tcgcccactt tcagagaacc cgcagcaagt ttaccgatgt gaccaatcgc
    15661 ctggccgtat ttctgcgtat cttccaccgc aaaggagaag ttagcgcctt tcagttcgcc
    15721 tttatcgcca acctgaccgc cggattccgc atagaatggc gtttgatcca gcacgaccac
    15781 agcttcctgg cctgcattga tggcatcaac cgctttacca tcaacaaaca gcgcagtcac
    15841 tttgccgttc agttccagat ggtcatagcc tttaaattca gatgcactgt caacacggat
    15901 cattgcgttg taatcggcac caaagccgct ggcttcgcgc gcgcgacgac gctgctcttc
    15961 cattgcagct tcaaaaccag cttcgtcaac tttgatgttg cgctcacgac aaacatcagc
    16021 cgtcaggtca accgggaagc cataggtgtc gtacagacgg aaagcagttt caccatccag
    16081 cgtatcacca gaaagttttg ccagctcttc atccagcaac gccagaccgc gctccagagt
    16141 acgagcaaac tgctcttctt cagtcttcag cacctgctca acctgcgcct gctggcgttt
    16201 caggtcttca cccgcagagc ccataacgtc gatcagcgga ccaaccagtt tgtagaagaa
    16261 ggtttctttc gcgccgagca tattaccgtg acgcactgcg cgacgaatga tacgacgcag
    16321 tacataacca cggttttcat tggacggcat tacgccatcc gcgatcagga acgcacaaga
    16381 acgaatgtgg tcagcgatta cgcgcagcga tttattgctc agatcggttg cgccagtgac
    16441 tttcgctacc gcctggatca gcgtgcggaa caggtcgatg tcatagttag agttaacgtg
    16501 ttgcagcacc gcagcaatac gctccagacc cataccggta tctacagacg gcttcggcag
    16561 cggttccatc gtgccatcgg cctggcggtt gaactgcatg aagacgatgt tccagatctc
    16621 aatgtagcgg tcgccgtctt cttccgggct tcccggaggg cccccccaaa tgtggtcgcc
    16681 gtgatcgtag aagatttcgg tgcacgggcc gcacggacca gtgtcaccca tctgccagaa
    16741 gttgtcagat gcgtatggcg cacctttgtt atcgccgatg cgaataatac gttcgcgcgg
    16801 gatccctact tctttttccc agatttcgta ggcttcgtcg tcgctttcat agacggtaac
    16861 ccacagacgc tctttcggca gggcaaacca tttttcgctg gtcagcagtt cccatgcaaa
    16921 ctgaatggca tcgtgtttga aatagtcgcc gaagctgaag ttgcccagca tttcgaagaa
    16981 ggtatggtga cgcgcggtgt aaccgacgtt ttccaggtcg ttgtgtttac cacccgcacg
    17041 cacgcagcgt tgggaagtgg tagcgcggga ataattacgc ttgtcgagcc caaggaacac
    17101 atccttgaac tggttcatcc cggcgttggt aaacaacaaa gttgggtcgt tatggggtac
    17161 cagggagctg ctggcaacta cctgatgtcc cttactatgg aaaaagtcga gaaacgcctg
    17221 acggatctca gcggtgctct tgctcataat tatcctgaaa tcaagctaac gaaatatcgc
    17281 caccagctcc agcgtgtctt aaccgccggg ctggtaactg aaaagtggga ataagataag
    17341 ttttcttgac tgggaagtaa aataccgtat gcgttcagtc ggcaaaattt cgccaaatct
    17401 cctggatatc ttccatcaga tagccacgat agagcagaaa acgctggatc ttaacttttt
    17461 ctgaaaagac agttggcaaa ggttcgccat attttcgcgt cgcctgatcg cgcgccagtg
    17521 cgcaccagtc gatgtcacat tcacgcatcg ctttttctgt cgcttcgcgg gaaatacctt
    17581 tctgattcag ttcctggcga atacgcgcag gtccataacc tttgcggcta cggctggcga
    17641 taaagcgcgc aacaaatcgg ctgtcatcga gatagccatg ttcatggcac caggcaataa
    17701 cgcgctcgta atcttctgcc gtagcatcaa tctcttctgg gccatttttg cccataatcg
    17761 gtgccgcgag tttacgtcgc agttcttgct cactgtgatc gcgcaccgcc agaatgcgta
    17821 ccgcacgatc caacaggcga gcatatgccg ggcgacggga tgttgattct gtcatggcat
    17881 atccttacaa cttaaaaaag caaaagggcc gcagatgcga cccttgtgta tcaaacaaga
    17941 cgattaaaaa tcttcgttag tttctgctac gccttcgcta tcatctacag agaaatccgg
    18001 cgttgagttc gggttgctca gcagcaactc acgtactttc ttctcgatct ctttcgcggt
    18061 ttccgggtta tctttcagcc aggcagtcgc attcgcttta ccctgaccga tcttctcacc
    18121 tttgtagctg taccacgcgc ctgctttctc gatcagcttc tcttttacgc ccaggtcaac
    18181 cagttcgccg tagaagttga taccttcgcc gtagaggatc tggaattcag cctgtttaaa
    18241 cggcgcagcg attttgttct tcaccacttt cacgcgggtt tcgctaccca ccacgttttc
    18301 gccctctttc accgcgccga tacgacggat gtcgagacga acagaggcgt agaatttcag
    18361 cgcgttacca ccggtagtgg tttccgggtt accgaacatc acaccaattt tcatacggat
    18421 ctggttgatg aagatcagca gcgtgttgga ctgcttcagg ttacccgcca gcttacgcat
    18481 cgcctggctc atcatacgtg ccgcaaggcc catgtgagag tcgccgattt cgccttcgat
    18541 ttccgctttc ggcgtcagtg ccgccacgga gtcaacgacg ataacgtcta ctgcgccaga
    18601 acgcgccagg gcgtcacaga tttccagtgc ctgctcgccg gtgtccggct gggagcacag
    18661 caggttatcg atatcgacgc ccagtttacg tgcgtagatt gggtccagcg cgtgttcagc
    18721 atcgataaac gcacaggttt taccttcacg ctgcgctgcg gcgatcacct gcagcgtcag
    18781 cgtggtttta ccggaagatt ccggtccgta gatttcgacg atacggccca tcggcagacc
    18841 acctgcccca agcgcgatat ccagtgaaag cgaaccggta gagatggttt ccacatccat
    18901 ggaacggtct tcacccaggc gcatgatgga gcctttacca aattgtttct caatctggcc
    18961 cagtgctgcc gccaacgctt tctgtttgtt ttcgtcgata gccattttta ctcctgtcat
    19021 gccgggtaat accggatagt caatatgttc tgttgaagca attatactgt atgctcatac
    19081 agtatcaagt gttttgtaga aattgttgcc acaaggtctg caatgcatac gcagtagcct
    19141 gacgacgcac cgcatcacgg tcgccgctga agcattcccg ccgggtaatg ccttcaccgc
    19201 gggcagtggc aaaagcaaac cagacggtgc cgacaggctt ctcttcactg ccgccatccg
    19261 gcccggcgat accactaata gacacggcat aatcagcacg agccgctttc agtgcgccta
    19321 tcgccatttc caccacgacg ggttcactca ccgcgccatg ctgcgccagc gtctcttcgc
    19381 gtacgccgat catctgcgct ttggcttcgt tactgtaggt gacaaatccg cgttcaaacc
    19441 aggcggagct accggcaata tcggtaatca ctttcgctac ccaaccaccg gtacaagact
    19501 cggcagttgt tacggttgcg ccacgggctt tcagcgcctg cccaacctgt tcacttaact
    19561 gcatcagttc actgtcagtc atcacaacct ctgtcagtca aaaatttatg ccggacaaga
    19621 tagcactttc gtcaggaaga tggtgttggc gttgcaattt tacgaggagg attcagaaaa
    19681 aagctgatta gccagaggga agctcacgcc cccctcttgt aaatagctac tgtactcgcg
    19741 ccagcgccac ggcttgtcct aactgccaga ccgccattgc gtaatgggtg ctgtggttgt
    19801 aacgggtgat ggtgtagaag ttcggcagac cgtaccagta ctggtagccg gtgccaacat
    19861 ccagacgcag caggctggct tgttgatggt tgcccagcgg ctgctgtggc gttaaacctg
    19921 cggcggcaag ctgcgaaatg ctgtacttag ttttgaagcc atttggcaag cctggagcct
    19981 gaccgtttgc cattaccgcg acctgatcgc ctttcaccca gccgtgcgct ttgaaatagt
    20041 tcgccacgct accgatcgca tcaaccggat cccacaggtt gatatgcccg tcgccgctga
    20101 aatctaccgc atattgtttg taagacgacg gcataaactg tccgtagccc atcgccccgg
    20161 caaaggaacc tttcagattg agcggatcgt cctgctcgtc gcgcgccatc aacaggaagg
    20221 tttccagctc gccagagaaa tactccgcgc ggcgtgggta gttaaatgac agcgttgcca
    20281 gcgcatcgag gatgcgagtt ttccccatca cgcgccccca gcgggtttca acgccgataa
    20341 tcccgacgat aatttccggc ggtactccat acacctgcca cgcgcgattc aacgcatctt
    20401 catactgatt ccagaaaacc acaccgttct gcacgttgtc cggcgtaata aattttttgc
    20461 gataacggag ccatgcgccg ttcggacctg atggcggttt caccgatgtg gttggtgcct
    20521 ggttatccat cagccgcagt accgaatcca gacgcttcgc ctgggagaga atttcctgca
    20581 actgctgacg atcgaaaccg tgtttgttca ccattctgtc gatgaactgc tgggcattcg
    20641 ggttattagc gaaatcgccg cccatctgca tcacattgtg ctgcggctca agcaggaagc
    20701 cgccagacgg cgttccggtg gtcgtatcag tctcagtagg ttttggcttg ctgctacagg
    20761 cggcaagcaa cacaaaaagg ggaagcaatg ttacataacg acgcttgaac atgaggggtc
    20821 catttaacag attcaaccag gggcaagtat ggtaaagcat cacgccccgc acaaggaagc
    20881 ggtagtcact gcccgatacg gactttacat aactcaactc attcccctcg ctatcctt
//
LOCUS       DAPHBJ010000069        20921 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.069, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000069 DAPHBJ010000000
VERSION     DAPHBJ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.069"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(73..285)
                     /gene="cspA"
                     /locus_tag="RHZ76_002267"
     CDS             complement(73..285)
                     /gene="cspA"
                     /locus_tag="RHZ76_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HDV2812902.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(566..856)
                     /locus_tag="RHZ76_002268"
     CDS             complement(566..856)
                     /locus_tag="RHZ76_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HDV2812903.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1290..2000
                     /locus_tag="RHZ76_002269"
     CDS             1290..2000
                     /locus_tag="RHZ76_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HDV2812904.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2050..3024)
                     /gene="ghrB"
                     /locus_tag="RHZ76_002270"
     CDS             complement(2050..3024)
                     /gene="ghrB"
                     /locus_tag="RHZ76_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HDV2812905.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3128..3787)
                     /locus_tag="RHZ76_002271"
     CDS             complement(3128..3787)
                     /locus_tag="RHZ76_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HDV2812906.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIVALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            3940..6273
                     /gene="bisC"
                     /locus_tag="RHZ76_002272"
     CDS             3940..6273
                     /gene="bisC"
                     /locus_tag="RHZ76_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HDV2812907.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6242..6682)
                     /locus_tag="RHZ76_002273"
     CDS             complement(6242..6682)
                     /locus_tag="RHZ76_002273"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV2812908.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6679..7242)
                     /gene="tag"
                     /locus_tag="RHZ76_002274"
     CDS             complement(6679..7242)
                     /gene="tag"
                     /locus_tag="RHZ76_002274"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDV2812909.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7400..8098
                     /locus_tag="RHZ76_002275"
     CDS             7400..8098
                     /locus_tag="RHZ76_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV2812910.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGLS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8327..9535
                     /locus_tag="RHZ76_002276"
     CDS             8327..9535
                     /locus_tag="RHZ76_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2812911.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALLMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            9859..11550
                     /gene="eptB"
                     /locus_tag="RHZ76_002277"
     CDS             9859..11550
                     /gene="eptB"
                     /locus_tag="RHZ76_002277"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HDV2812912.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVSMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEAAAN"
     gene            11642..11718
                     /locus_tag="RHZ76_002278"
     tRNA            11642..11718
                     /locus_tag="RHZ76_002278"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11676..11678,aa:Pro,seq:cgg)
     gene            11753..11886
                     /locus_tag="RHZ76_002279"
     ncRNA           11753..11886
                     /ncRNA_class="other"
                     /locus_tag="RHZ76_002279"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            12629..14236
                     /gene="dppA"
                     /locus_tag="RHZ76_002280"
     CDS             12629..14236
                     /gene="dppA"
                     /locus_tag="RHZ76_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HDV2812913.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(14299..14526)
                     /locus_tag="RHZ76_002281"
     CDS             complement(14299..14526)
                     /locus_tag="RHZ76_002281"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812914.1"
                     /translation="MTRSLELRVCIAHKSIPALIGQGMKYCCRMALHAEAQRHRVYRL
                     LTKCALFMPDAALTPYPAYKIVQIQYIAEFS"
     gene            14544..15563
                     /gene="dppB"
                     /locus_tag="RHZ76_002282"
     CDS             14544..15563
                     /gene="dppB"
                     /locus_tag="RHZ76_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HDV2812915.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15573..16475
                     /gene="dppC"
                     /locus_tag="RHZ76_002283"
     CDS             15573..16475
                     /gene="dppC"
                     /locus_tag="RHZ76_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HDV2812916.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16486..17469
                     /gene="dppD"
                     /locus_tag="RHZ76_002284"
     CDS             16486..17469
                     /gene="dppD"
                     /locus_tag="RHZ76_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDV2812917.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17466..18470
                     /gene="dppF"
                     /locus_tag="RHZ76_002285"
     CDS             17466..18470
                     /gene="dppF"
                     /locus_tag="RHZ76_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HDV2812918.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIEMPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18500..19771)
                     /locus_tag="RHZ76_002286"
     CDS             complement(18500..19771)
                     /locus_tag="RHZ76_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDV2812919.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20247..20354
                     /locus_tag="RHZ76_002287"
     CDS             20247..20354
                     /locus_tag="RHZ76_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDV2812920.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            20730..20837
                     /locus_tag="RHZ76_002288"
     CDS             20730..20837
                     /locus_tag="RHZ76_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDV2812921.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         4999 a         5216 c         5558 g         5148 t
ORIGIN      
        1 atttcctgaa aacaaataaa aaaatccccg ccaaatggca gggatcttag attctgtgct
       61 tttaagcaga gattacaggc tggttacgtt accagctgcc gggcctttag cgccgctttc
      121 gatggtgaag gacactttct gaccttcgtc cagagatttg taaccatcgt tctggatagc
      181 agagaagtgt acgaacacat ctttagagcc atcgtcagga gtgatgaagc cgaagccttt
      241 gtcagcgttg aaccatttta cgataccagt cattttaccg gacatagtgt attaccttta
      301 ataattaagt gtgcctttcg gcgatatggc gtgctttaca gattttgaag cgttaaagga
      361 atgtgcacta cgaggggtat caacgataac tcttgaaggg acttgcctta ctacactgct
      421 ttaatggtct gtacgtcaaa ccgttgatgt gcattaagcc acgcattggc gggtgatgca
      481 acaattattt ttcatattta tgattaatcg gttggaaaac ggtcctgtca tcaggaccgt
      541 aaacagcaat aaagtggata aaagtctatt ccatcaactg cttacttaat gccgggttgg
      601 cttgaatcaa acgcatcaat tttagttcgg cacttgaagg cttcacgcgt ctggattccc
      661 attcctttac catggcgact gatacgccca aaacccgggc gaaatcatcg atttttaatc
      721 ctgtcccttt tcgtaactgc tcaatttcgg tacaggacgt tgttctgtgc gtcagggtaa
      781 ttttctgcgt ttcatcttta aaaacaatct gttccaggct gctcaacagc tcatgcattg
      841 gatctttata ttccattgaa aactcctcaa atcacactgc gggatcgtga attgcatcga
      901 agctcattaa gaatagtcgg gaaaagcaga cacagcgggg tgaagtcagg gcatgctctg
      961 gctatgattc gattcagcct gtcatttagc gtgacttata gccacctaac gatctgataa
     1021 atgccgatat atctcttacc actcctgttt tgtaaagatt tcgaaaatct aaccttctgc
     1081 gaaggcgtaa atctctggct acaaaaaagg caacggcact atcatttttt aacaatgaaa
     1141 caacaaatta accttgtttc attttctcca tgattttacc aggaagatta cgtagaaagg
     1201 gtatcctgca agcctgtcct ggactatcct tgtcacgtca gacacgcgtg tgtcgttgtg
     1261 cgcttttttt gggtgaaagg agtaagaaaa tggcgacagg aaagtcctgc tctcgctggt
     1321 ttgcgcctct tgcggcgtta ttaatggtag ttagcctgag tgggtgtttt gataaagaag
     1381 gcgatcagcg taaagcgttt atcgacttcc tgcagaatac agtgatgcgt agcggtgaac
     1441 gtctaccaac cctgactgcc gatcagaaaa aacagtttgg tccttttgtc tctgattacg
     1501 cgattctgta tggttattct cagcaggtga atcaggcgat ggattccggt ctgcgcccgg
     1561 ttgtagacag cgttaacgca attcgcgtac cgcaggatta cgttacgcaa agcggtccgc
     1621 tgcgtgaaat gaacggttcg ctgggtgtgc tggcgcaaca actgcaaaat gcgaagttgc
     1681 aggccgatgc tgcgcactct gcgctaaaac agagcgatga tttgaaaccg gtctttgatc
     1741 aggcattcac caaagtggta acgacgccag ctgatgcatt gcaaccgttg attccggcgg
     1801 cacaaacttt cacgcaacaa ctggtgatgg ttggagacta cattgctcag caaggtactc
     1861 aggtgagctt tgtcgcaaat ggcatccagt tcccgacttc acagcaggca agtgaatata
     1921 acaaactgat tgcgccatta ccggcacagc atcaggcgtt taatcaggcc tggactacag
     1981 cagttactgc aacccaataa agagtaaaag cccgagcgat tatcgggctt tactctacgc
     2041 agtcgcggct tagtccgcga cgtgcggatt cacacagttc ttctcaacct ttccttgtaa
     2101 cgcatcaatc aaattatcca cggcacaggc ggccatgcca taacgcgtct catgggtggc
     2161 agatccaata tgcggtactg cgacgacgtt ggccattgag agcaacggcg aatctacgga
     2221 cagtggctct tgttcgaaga catccagccc ggcagcgtga atttcgcctt tctgcaatgc
     2281 tgcgatcagt gcattttcgt caaccaccgg gccacgtccg gcattaatga aaatggcgga
     2341 ggatttcatt ttggcgaatt gttctgcgcc aaacagatga tgcgtctcat cagttaacgg
     2401 caggatcagg caaacgaaat ctgactcttg taacagagta tccaaatcgc agtagcgggc
     2461 gttgaagcgt tcttctgctt ctttatggtg gcggcgcgcg ttatagagga tgggcatgtt
     2521 gaagccaaag tgcgcacgtt gtgccagcgc catgccgatc cgtcccatcc cgacaatgcc
     2581 cagtgtttta tggtgaacgt cagtgccgta ccagtccggg cctatgctcg cggtccattc
     2641 gcctgctttt acccgttctg ctacctccac aacccgacga gcggtagaca acaccagcgc
     2701 catcagcgta tcggcgacgg tttctgttaa tacggttggc gtgtgcatca gcagaatttt
     2761 tcgggcggta agcgcatcga catcaaaatt gtcatagccg acggagatcg ttgatgtggc
     2821 acgcagtttc ggcatttttt ccagcaatgc ggcatcaaca ttctcgtttg aacccagtaa
     2881 accttcagct tcggcaaaaa ttgctgcatt ttgttcgacg gtttgtgggc tgaggtttgc
     2941 cacctggtga acggtgaaat gctcttgcag gcgttgcagt aaatcatcag gtaaggcttt
     3001 gtagaggata acggacggct tcatgcttct ctccattcac tgataaggcg atcaccttag
     3061 cctgaaatat gatgaaggaa aactgaagaa tgccaggtga tttctgcatc acctggcatg
     3121 aaagggatta cagcgggctt aaggtaattt ctacacggcg gttttgcgcc ttaccttctg
     3181 cggtgctgtt gctggcgatt gggttagccg ggccaaggcc ctgagtacgg atgcggctgg
     3241 cgtccacgcc ctgggtgatc aacgcgctgg caacggaatc cgcacgctgc tgggagagac
     3301 gcatgttcag gtcgtgaccc cccgtgctgt cggtataacc aatcacgtta accgccgttt
     3361 tcggatactc tttcagcacc attgccacgc cggtcagggt gttagcgcct gccggtttca
     3421 gagtcgcgct gctgctgtcg aaggtcacat tgttcggcat attgaggata atgttatccc
     3481 cgctgcgggt tacgctaaca ccagtgccgc gcattttgtc gcgcagcttc gcttcctgca
     3541 catccatgta ataaccaacg ccgccgccca gagctgcgcc tgctgctgcg ccaatcagcg
     3601 cgcctttacc gcgatctttc ttcgaagaag agagcgcaac aatacccgcg cccacgagag
     3661 agcccagacc tgcgccgata gcagatttac ctgcttcgcg ttcgccggtg taagggttag
     3721 ttgtgcagcc agataccgcc agagcgccac tcactacggc ggcaataaga taaacacgtt
     3781 tcttcattgt taatccttaa taaccttttt attctttgcc acgggttccg tggcgggaga
     3841 ttatgccgcg tgaacatgaa gattattcct gggaatactc ggaaatttgt aagtaatatt
     3901 taactgctca atacatctaa cctttcagga gtcttcggtt tggccaactc atcctcacga
     3961 tattccgttc tgactgccgc ccactggggg cccatgctgg ttgaaaccga cggcgaaacc
     4021 gtgtttagct cgcgtggcgc gttagccaca ggaatggaaa actccttgca gagcgcggtt
     4081 cgcgaccagg ttcacagcaa tacgcgggta cgatttccaa tggtgcgcaa aggctttctc
     4141 gcgtcaccgg aaaacccgca aggcattcgt gggcaggatg aatttgttcg cgtgagttgg
     4201 gatgaggcgc tggatcttat tcaccaacaa cataaacgca ttcgtgaggc ttatggtccg
     4261 gcatcgattt ttgctggttc ctacggctgg cgttcaaacg gcgtgctgca taaggcctcg
     4321 acattattac aacgctatat ggcgctggca ggcggttata ccgggcatct gggggattat
     4381 tcgaccggcg cggcacaggc gatcatgccg tatgtcgtgg gtggtagcga agtttatcaa
     4441 cagcagacca gttggccgct ggtgctggaa catagcgatg tcgtggtgct gtggagtgct
     4501 aatccactca atacgctgaa aattgcgtgg aatgcatccg atgagcaggg gctttcttac
     4561 ttttctgcac tgcgtgacag tgggaaaaag ctgatctgca ttgatccaat gcgatcggaa
     4621 accgtcgatt tctttggcga taaaatggag tgggtggcac cgcacatggg caccgatgtt
     4681 gcgctgatgc tggggatcgc ccatacgctg gtggaaaatg gttggcacga cgaagcgttt
     4741 ctggcgcgtt gcaccacagg ttatgccgtc ttcgcctctt atttgctggg cgagagtgac
     4801 ggaatagcga aaaccgccga atgggcagca gagatttgtg gtgttggcgc agcgaaaatc
     4861 cgcgagctgg cggctatttt ccaccaaaat accaccatgc tgatggcagg ctggggaatg
     4921 cagcgccaac agtttggtga gcaaaaacac tggatgatcg tcacgctggc agcaatgttg
     4981 gggcaaatcg gcacacccgg cggcggtttt ggtctttctt accattttgc caatggtggt
     5041 aaccccacgc ggcgttctgc ggtgctctct tccatgcagg gcagcttgcc gggtggctgc
     5101 gatgcggtag ataaaatccc tgtggcccgc attgttgaag cactggaaaa ccctggtggc
     5161 gcatatcaac acaacggtat ggaccgacat tttccggata ttcgttttat ctggtgggcg
     5221 ggcggtgcca actttactca tcatcaggat accaaccgcc tgatccgtgc ctggcaaaaa
     5281 ccggagctgg tggtgatctc cgaatgtttc tggacggcgg cggcgaaaca cgcggatatc
     5341 gttctgcctg cgaccacctc gtttgagcgt aatgatctca ccatgaccgg cgattacagt
     5401 aatcagcatc tggtgcccat gaagcaagtg gtgccgccac gctatgaagc gcgtaatgat
     5461 tttgatgttt ttgccgagtt aagtgaacgc tgggagaagg gcggttatgc acgttttacg
     5521 gaaggaaaaa gtgagctgca atggctggaa acgttttata acgttgcccg acagcgcggg
     5581 gcaagccagc aggttgaatt gccgccattt gctgagttct ggcaagccaa ccagttaatt
     5641 gagatgccgg aaaacccgga cagcgagcgg tttattcgct tcgctgattt ttgccgcgat
     5701 ccgcaggcgc atccattaaa aaccgccagc ggtaagattg aaatcttctc gcagcgtatt
     5761 gccgattacg cttatccgga ttgcccgggg catccaatgt ggctggagcc ggacgaatgg
     5821 cagggcaatg ccgaaccaga acagttgcag gtactttctg cccatccggc gcaccgcctg
     5881 cacagccagc tgaattacag ttctctgcgc gaattgtacg cggtagcaaa ccgtgagcct
     5941 gtcaccattc atcctgacga tgctcaggcg cgcggcataa ccgaagggga tatggtgcgg
     6001 gtgtggaact cccgtgggca gatccttgcc ggagcggtca ttagcgaggg aattaaacct
     6061 ggcgtgattt gcattcacga aggggcatgg ccggatctgg atttaaccgc tgacggtatt
     6121 tgtaaaaacg gcgcagtgaa cgtgctgacc aaagatctcc ccagctcgcg gctggggaat
     6181 ggctgtgcgg gtaatacggc gctggcatgg ctggaaaaat acaacggtcc ggaactgaca
     6241 cttacagcgt ttgaaccacc ggccagctca taatccaggt gggtagttgg gtttcatcct
     6301 gccatgcgca atcgacaatg tgaaaaccct gcgcacggta aaaatctatc gccggttgat
     6361 ttttttgata aacctccagc atcagatggg gatagcgctg ctgcacatac tgcatcagcg
     6421 ccttaccaat accgcgtctg acggccttcg gtgcgacaaa cattgctgcc agaaatcggc
     6481 cttccataat gctgacaaaa ccgagaagct taccgtcctc ttcccagacc cagttttgcg
     6541 cattggcaag ataggcatcc cgcaccagcg gaatgcagtc acgccagtaa ttcgctttta
     6601 taaagggatg cccccaggtt gtactttcca gccacagttc gaggatcgcg gggagttctg
     6661 aacgttgcgc ttcccgaatc atggtttatt tcccggatag caacagcagc caaccacatg
     6721 atcattcacc agcccacatg cctgcataaa ggagtaacag attgtggtgc cgacaaactt
     6781 aaaaccacgt tttttcagtg ccttagatag ggcgtcggag gcggacgtag atgtgggaat
     6841 ttcgctcaac gttgtggctt gtgtcacctg tggctgatga tttacaaacg accagacaaa
     6901 gtcggcaaac ggttcgccgt tctgttccat ttgcaggtac gcccgcgcat taccaataat
     6961 tgcctgaatt ttccctcgat ggcggataat cccggcgtcc tgtaccagtc tttcgacatc
     7021 ctcttcctgc attgcggcaa ccttcaccgg atcgaactga tgaaagcagg cgcgatagtt
     7081 ttcgcgtttt ttgaggacgg tgatccacga taacccagcc tgctgccctt caaggcagat
     7141 catttcgaac agttttttac tgtcagtttc aggcacgccc cactcattat catggtaggc
     7201 aatataaagc gggtcctgac tcacccagcc gcaacgttcc atactttccc tcgctatgta
     7261 cccgatcgta aaaatttcag ccgcttttct tgacgcatcg gtgaaaaagt caatatttaa
     7321 tacagggcta ctatgcccga tatccttcat acaatgacaa taatattgcc gaattcggct
     7381 cttccctgga gtcgtgttaa tgatcataaa aaaaagcggt ggtcgctggc agctaagcct
     7441 gctggcgagc gtggtaatca gtgccttttt tctcaacaca gcttacgcct ggcaacaaga
     7501 atatatcgtt gatacgcaac ccggactttc cacagagcgt tacacctggg atagtgatca
     7561 tcaacctgat tacaacgata ttttgtcgca acgtattcaa agtagccaaa gggcgctggg
     7621 actggaagtc aatctggcgg aagaaacccc tgtggatgtg accagcagta tgagtatggg
     7681 ctggaatttt cctttatatg aacaggttac aaccggcccg gtcgcggcat tacattacga
     7741 tggcacaacc acctcgatgt ataacgagtt tggcgacagt actaccacgc tgaccgatcc
     7801 gttatggcat gccagcgtga gtaccttagg ctggcgtgtt gactcccggc ttggcgatct
     7861 ccgaccctgg gcgcaaatca gctataacca gcaatttggc gagaatatct ggaaggcgca
     7921 atcaggcctg agccggatga cggcgacaaa ccagaacggc aactggctgg atgtcaccgt
     7981 aggcgctgat atgttgctca atcaaaatat tgccgcctat gccgcgctaa ctcaggcaga
     8041 aaataccact aataatagcg actatctgta tacgatgggg gttagcgcca gattttaacg
     8101 taacagtcac aattgaaacc attaaataac aatagttgtg gcgatagtgg gtgctaactt
     8161 accaaataat aaatttggtg aataattgtc gcgtcattca ttcctgaact aaggcatttc
     8221 attccgttct gatggcattt catgccgttt ttccccaggc ataaagtgca cttcgttatg
     8281 gttgtcggca gagatttttc ctttttatta ctgcaggaat actgccatga caccttcaaa
     8341 ttatcagcgt acccgctggc tgacactcat cggtactatc attacccagt ttgcgctggg
     8401 gtcggtttat acctggagcc tgtttaatgg cgcgctttcc gccaagctgg atgcgccggt
     8461 aagccaggtc gctttctctt tcggcttgtt aagtctgggg ctggcaattt cgtcttctgt
     8521 tgcgggcaaa ttacaggaac gttttggcgt taaacgcgtc accatggctt ccggcatttt
     8581 gctgggatta ggcttcttcc tgacagcgca ttctgacaac ctgatgatgc tgtggttaag
     8641 cgccggtgtg ctggtgggac tggcagatgg cgcgggttat ctgctgacgc tctctaactg
     8701 tgtgaagtgg ttcccggagc gtaaaggtct gatctccgcg ttcgctatcg gttcttatgg
     8761 tctgggtagc ctgggtttca aatttatcga cacgcagctg ctggaaacgg tcggtctgga
     8821 aaaaaccttt gtgatttggg gagcgattgc gctgttgatg attgttttcg gcgcaacgtt
     8881 aatgaaagac gcaccaaaac aggaagtgaa aaccagcaat ggtgtggtgg agaaagatta
     8941 cacgctggca gagtcgatgc gtaaaccgca gtactggatg ttagcggtaa tgttcctgac
     9001 cgcctgcatg agcggcctgt acgtgattgg ggtagcgaaa gatatcgccc aaagtctggc
     9061 acaccttgat gtggtttccg cagccaatgc agtcactgtt atttccatcg ccaacctttc
     9121 aggtcgtctg gtgctgggta ttctgtctga caaaatcgcc cgtatccgtg ttattaccat
     9181 tggtcaggtg atatcgctgg tgggtatggc ggccctgctg tttgcaccat tgaatgcagt
     9241 gacgttcttt gcagcgattg cctgcgtggc atttaacttt ggcggcacta ttaccgtctt
     9301 tccgtcactg gtcagtgagt tctttggcct caataacctg gcgaaaaact acggtgtgat
     9361 ttatctcggt ttcggtatcg gtagcatttg tggttcgatt atcgcctcac tgtttggcgg
     9421 cttctatgtg accttctacg tgattttcgc cctgctgatt ctgtcattgg cgctttctac
     9481 gacgattcgt cagccagagc agaaaatgtt gcgtgaggcg catggctccc tttaatgctc
     9541 agccgcgagc tactgggctt cagcttccgt gtgcatcaaa cgcatcaacg aaatgtataa
     9601 acaatgactc gtaaaatttg ggccaattaa ctggcccttt ttactgcttt ttgtaacgct
     9661 aaagtctctt ttcaaacttg catttttgta aatttgtgct tcatgcacac tctttcccca
     9721 cactttttcc ctttgctgtg gtctacttat tcgcgcgtgt agattttact tatctgacta
     9781 cctccgcact ttttccctgc cgggcctgaa aagccactaa gcagggtgtt atcacctgtt
     9841 tgtccagggt ttgtttgcat gagatacatc aaatcgatta cacagcagaa gctgagcttt
     9901 ttgcttgcaa tctatattgg cctttttatg aatggcgcgg ttttttaccg ccgcttcggc
     9961 agctatgcgc acgattttac cgtctggaaa ggcatttctg ctgttgttga actggccgcc
    10021 accgtactgg tgaccttctt tttactacgt cttctttcgc tgtttggccg ccgcagctgg
    10081 cgtattctgg catcgctggt ggtgctcttt tccgcaggtg ccagctatta catgaccttc
    10141 cttaatgtgg tcattggtta tggcatcatc gcttccgtca tgaccaccga tatcgacctg
    10201 tcaaaagaag ttgttggtct gaactttatt ctctggttaa tcgccgttag tgcattgcct
    10261 cttatcctta tctggaataa ccgctgtcgc tacaccttgc tccgacaact gcgaacccca
    10321 gggcagcgta ttcgcagcct ggcggtcgtc gtactggcgg gtattatggt ttgggcaccg
    10381 attcgtttgc tggatatcca gcagaagaaa gtggagaggg cgaccggcgt tgatttgccg
    10441 agttatggcg gtgtcgtagc gaactcttat ctgccatcaa actggctttc tgcgttgggg
    10501 ctgtatgcct gggcgcgggt cgatgaatct tccgataata attcattgct taatccggcg
    10561 aagaaattca cttatcaggc accgcaaaac gttgatgaca cttatgtcgt gtttatcatc
    10621 ggtgaaacca cgcgttggga ccatatgggt attttcggct atgagcgtaa taccacgccg
    10681 aaactggccc aggagaaaaa tctggcggcg ttccgtggtt actcctgtga taccgcaacc
    10741 aaactctcac tgcgttgcat gtttgtacgt caggggggcg cggaagataa tccgcagcgc
    10801 acattaaaag aacagaacat tttcgcggtt ctgaagcagt taggattcag ttctgacctc
    10861 tacgctatgc agagcgaaat gtggttctac agcaacacga tggcggacaa cattgcttat
    10921 cgtgagcaga ttggtgcgga gccacgtaat cgtggcaagc cggtagatga tatgttgctg
    10981 gtagacgaaa tgcagcaatc gctagggcgc aacccggatg gtaagcatct gatcattctg
    11041 cataccaaag gttcgcattt taactacacc cagcgttatc cgcgtagctt cgcgcagtgg
    11101 aagccggaat gtattggtgt tgatagcggc tgtaccaaag cgcagatgat caactcctat
    11161 gacaactcgg tgacctatgt ggatcacttt atctccagcg tgattgatca ggttcgcgat
    11221 aagaaagcga ttgtgttcta cgcagctgac cacggtgagt caattaatga acgcgagcac
    11281 ctgcacggca cgccgcgtga actggcaccg ccggagcagt tccgcgtatc gatgatggtc
    11341 tggatgtcag ataaatatct ggaaaatccg gccaatgcgc aggcgtttgc gcagctgaaa
    11401 aaagaagccg acatgaaagt gccacgccgt cacgtagagc tgtacgatac catcatgggt
    11461 tgtcttggct atacttcacc ggatggtgga attaacgaaa acaacaactg gtgtcacatc
    11521 ccgcaggcaa aagaggcagc ggctaactaa gcggcctgct gactttctcg ccgatcaaaa
    11581 ggcattttgc tattaaggga ttgacgaggg cgtatctgcg cagtaagatg cgccccgcat
    11641 tcggtgattg gcgcagcctg gtagcgcact tcgttcggga cgaaggggtc ggaggttcga
    11701 atcctctatc accgaccaaa ttcgaaaagc ctgctcaacg agcaggcttt tttgcatctg
    11761 cagctcatga ggatgagaac ctccgggggc agggaggttc gactcgagcg aagcgagaga
    11821 acgttgcgcc agcaacggcc cgcagggcga gccacgaagt ggcgagtaat cctctatcac
    11881 cgaccaaatt cgaaaagcct gctcaacgag caggcttttt tgcatctgca gctcatgagg
    11941 atgagaacct ccgggggcag ggaggttcga ctcgacgtag gcctgataag acgcgtcagc
    12001 gtcgcatcag gcaaatgtgc cggccattgt cggatgcggc gcgaccgcct tattcgacct
    12061 acacgacaca catttccctg ccaaatttgc gccccatatc tcactatcca ttaacggatt
    12121 tgtgacagaa accattgtct ttttttatat ttgcttaaat ctttttttgc gttacttatg
    12181 gctggccctg gcattttccg ctgtgcgttt tagcgttcat ggtattaatc gctcagatat
    12241 ccaccttttc ctcagatttt tttcattaaa tgcataaaac ttaatcacca aatgttgcta
    12301 aatattaagc tgattgttct gttcatgttg gagtagcaca aatttctctg ctgcaaatag
    12361 ctgtttgaag tttttttaat ctttgtttgt ggattctcac cgttggtgta aatcccggtt
    12421 ggctgtattg acgttttcac attctgttga cagattgtag gtcacgaggg gcattttatg
    12481 gaggatccgc actgttacac tgatgttaat tagtacggca tccccacctc ataacgttga
    12541 cccgaccggg caaaaaacaa aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa
    12601 caacaaacat cacaattgga gcagaataat gcgtatttcc ttgaaaaagt cagggatgct
    12661 gaagcttggt ctcagcctgg tggctatgac cgtcgcagca agtgttcagg ctaaaactct
    12721 ggtttattgc tcagaaggat ctccggaagg gtttaacccg cagctgttta cctccggcac
    12781 cacctatgac gcctcttccg tcccgcttta taaccgtctg gttgaattta aaatcggcac
    12841 caccgaagtg atcccgggcc tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta
    12901 taccttccat ctgcgtaaag gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg
    12961 tgaactgaac gccgatgatg tggtgttctc gttcgatcgt cagaaaaacg cgcaaaaccc
    13021 gtaccataaa gtttctggcg gcagctacga atacttcgaa ggcatgggct tgccagagct
    13081 gatcagtgaa gtgaaaaagg tggacgacaa caccgttcag tttgtgctga ctcgcccgga
    13141 agcgccgttc ctcgctgacc tggcaatgga cttcgcctct attctgtcaa aagaatatgc
    13201 tgatgcgatg atgaaagccg gtacaccgga aaaactggac ctcaacccaa tcggaaccgg
    13261 tccgttccag ttacagcagt atcaaaaaga ttcccgtatc cgctacaaag cgtttgatgg
    13321 ctactggggc accaaaccgc agatcgatac gctggttttc tctattaccc ctgacgcttc
    13381 cgtgcgttac gcgaaattgc agaagaatga atgccaggtg atgccgtacc cgaacccggc
    13441 agatatcgct cgcatgaagc aggataaatc catcaatctg atggaaatgc cggggctgaa
    13501 cgtcggttat ctctcgtata acgtgcagaa aaaaccactc gatgacgtga aagttcgcca
    13561 ggctctgacc tacgcggtga acaaagacgc gatcatcaaa gcggtttatc agggcgcggg
    13621 cgtatcagcg aaaaacctga tcccgccaac catgtggggc tataacgacg acgttcagga
    13681 ctacacctac gatcctgaaa aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg
    13741 tttctccatc gacctgtggg cgatgccggt acaacgtccg tataacccga acgctcgccg
    13801 catggcggag atgattcagg cagactgggc gaaagtcggc gtgcaggcca aaattgtcac
    13861 ctacgaatgg ggtgagtacc tcaagcgtgc gaaagatggc gagcaccaga cggtaatgat
    13921 gggctggact ggcgataacg gggatccgga taacttcttc gccaccctgt tcagctgcgc
    13981 cgcctctgaa caaggctcca actactcaaa atggtgctac aaaccgtttg aagatctgat
    14041 tcaaccggcg cgtgctaccg acgaccacaa taaacgcgtt gaactgtaca aacaagcgca
    14101 ggtggtgatg cacgatcagg ctccggcact gatcatcgct cactccaccg tgtttgaacc
    14161 ggtacgtaaa gaagttaaag gctatgtggt tgatccatta ggcaaacatc acttcgaaaa
    14221 cgtctctatc gaataattaa aagccataca agactgatgg caaaggcaaa aatgcctgat
    14281 gcgctccgct tatcaggcct acgaaaattc tgcaatatat tgaatttgca cgattttgta
    14341 ggccggataa ggcgttaacg ccgcatccgg cataaacaaa gcgcactttg tcaacaatct
    14401 gtatacccgg tggcgctgtg cctctgcgtg cagcgccatc cggcagcaat acttcattcc
    14461 ctgtccgata agggcgggaa ttgatttgtg agcaatacag acacgcagtt ccaggctgcg
    14521 ggtcactata gagaatccgg gttatgttgc agtttattct ccgacgtttg ggactcgtca
    14581 tccccacgtt tatcggtatt acccttctca catttgcctt tgtccacatg atcccgggcg
    14641 atccggtgat gatcatggcg ggcgaacgtg ggatctcccc agagcgtcac gcgcagctgc
    14701 tggctgaact cggcttagat aaaccgatgt ggcagcagta tctccattac atttggggcg
    14761 ttatgcatgg cgatctaggc atttcaatga aaagccgcat cccggtttgg gaagagttcg
    14821 tgccgcgctt ccaggccacg ctggaacttg gcgtctgcgc gatgattttt gctacggcag
    14881 tcggtattcc ggtcggcgtg ctggctgcgg ttaaacgcgg ttccattttc gatcacacag
    14941 cggttggcct ggcgctgaca ggttattcaa tgcctatctt ctggtggggc atgatgctga
    15001 tcatgctggt ttcggtgcac tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg
    15061 tgttcctcga tgactccaat ccgttaaccg gttttatgct aatcgacacc gccatctggg
    15121 gtgaagacgg caactttatc gatgccgtcg cccatatgat cttgcctgcc attgtgctgg
    15181 gtactattcc gctggcggtc attgtgcgta tgacacgctc ctcgatgctg gaagtgctgg
    15241 gcgaggatta catccgcacc gcgcgcgcca aagggctaac ccgcatgcgg gtgattatcg
    15301 tccatgcgct gcgtaacgcg atgctgccgg tggtgaccgt tatcggcctg caggtgggaa
    15361 cattgctggc gggggcgatt ctgaccgaaa ccatcttctc gtggcccggt ctgggacgct
    15421 ggttgattga cgcactgcaa cgccgcgact atccggtagt gcagggcggc gtattgctgg
    15481 tggcgacgat gattatcctc gtcaacttgc tggtcgatct gctgtacggc gtggtgaacc
    15541 cgcgtattcg tcataagaag taaggggaca tcatgtcaca ggttactgaa aataaagtga
    15601 ttagcgcacc ggtgccgatg accccgttac aggagttctg gcactatttt aaacgcaaca
    15661 aaggcgcggt cgtcgggctg gtttacgtcg tcatcgtgct gttcatcgcg atctttgcca
    15721 actggattgc accctataac ccggcggaac agttccgcga tgcactgctc gccccgccag
    15781 cctggcagga aggcggcagc atggcgcact tgctgggcac cgatgacgta ggccgtgatg
    15841 tgctgtcgcg cctgatgtac ggtgcgcgcc tgtcgctgct ggttggctgt ctggtagttg
    15901 tgttatcgct gattatgggc gttattctcg gcctgatcgc cggttacttt ggcggcctgg
    15961 tcgataacat cattatgcgc gtggtcgata tcatgctggc gctgccaagt ctgctgctgg
    16021 cgctggtgct ggtggcaatt ttcggcccgt cgattggtaa cgccgcgctg gcactgacct
    16081 tcgttgcctt gccgcactat gtgcgcttaa cccgcgccgc cgtgctggtg gaagttaacc
    16141 gcgattacgt caccgcgtct cgcgtggcgg gtgccggggc gatgcgtcag atgtttatta
    16201 acatcttccc gaactgcctt gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg
    16261 ccattctcga tatggctgct cttggtttcc tcggcatggg ggcacagccg ccaacgcctg
    16321 agtggggcac catgctctcc gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga
    16381 ccttcccggg tctggcgatc ctgctgacgg tgctggcatt taacctgatg ggtgacggtc
    16441 tgcgtgacgc gctcgatccc aaactgaagc agtaagaggt tcgagatggc gttattaaat
    16501 gtagataaat tatcggtgca tttcggcgac gaaagcgcgc cgttccgcgc cgtagaccgc
    16561 atcagctaca gcgtaaaaca gggcgaagtg gtcgggattg tgggtgagtc cggctccggt
    16621 aagtcggtca gttcactggc gattatgggg ctgattgatt atccgggccg cgtaatggca
    16681 gaaaaactgg agtttaacgg ccaggatttg cagcgtatct cagaaaaaga gcgccgcaac
    16741 ctggtgggtg ccgaagtggc gatgatcttc caggacccga tgaccagcct taacccgtgc
    16801 tacaccgtgg gtttccagat tatggaagcg attaaggtgc atcagggcgg caacaaaagt
    16861 acccgccgtc agcgagcgat tgacctgctg aatcaggtcg gtattcccga tccggcatcg
    16921 cgtctggatg tttacccgca tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc
    16981 atggcgattg cctgtcggcc aaaactgctg attgccgatg aaccgaccac cgcgctggac
    17041 gtgaccattc aggcgcaaat catcgaacta ctgctggagc tacagcagaa agagaacatg
    17101 gcgctggtgt taattaccca tgacctggcg ctggtggcgg aagcggcaca taaaatcatc
    17161 gtgatgtatg caggccaggt ggtggaaacc ggtgatgcgc acgccatctt ccatgcgccg
    17221 cgtcacccgt atactcaggc attgctgcgt gcgctgccag aatttgctca ggacaaagaa
    17281 cgtctggcgt cgttgccagg tgtcgttccc ggcaagtacg accgcccgaa cggctgcctg
    17341 cttaacccgc gctgccccta tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat
    17401 atgctcgctg acgggcgtca gtccaaatgc cattacccac ttgatgatgc cgggaggccg
    17461 acactatgag tacgcaagag gccaccctgc aacaaccgct gttgcaggct atcgacctga
    17521 aaaaacatta tccggtgaag aaaggcatgt tcgcgccgga acgtctggtt aaagcgctgg
    17581 atggcgtttc gtttaacctt gaacgtggca aaacgctggc agtagtgggc gaatctggct
    17641 gcggtaaatc gaccctcggt cggttgctga cgatgattga aatgcccacc ggtggcgagc
    17701 tgtattacca ggggcaggat ctgcttaagc acgatccgca ggcgcagaag ctgcgtcggc
    17761 agaaaatcca gatcgtcttc cagaaccctt acggttcgct gaatccgcgt aaaaaagtcg
    17821 ggcaaattct tgaagagccg ctgctgatca acaccagctt aagcaaagaa cagcgtcggg
    17881 aaaaagccct gtcgatgatg gcgaaagtcg gcctgaaaac cgagcactat gaccgctatc
    17941 cgcatatgtt ctccggcggt cagcgtcagc gtatcgccat cgcccgtggt ctgatgctcg
    18001 acccggatgt ggtgattgcc gatgaaccgg tttccgcgct ggatgtttca gtgcgcgcgc
    18061 aggtgctgaa tctgatgatg gatttgcagc aggagttggg gctgtcttat gtctttatct
    18121 cccacgacct gtcggtggtg gagcacattg ctgatgaagt gatggtgatg tacctgggcc
    18181 gctgcgtgga gaagggaacg aaagaccaaa tcttcaataa cccgcgccat ccgtacactc
    18241 aggcgctact ttccgcgacg ccgcgcctga acccggacga tcgccgcgag cgcatcaagc
    18301 tcagcggtga actaccaagc ccactgaatc caccgccggg ttgcgccttc aacgcccgct
    18361 gtcgtcggcg cttcggcccc tgcacccagt tgcagccgca gctaaaagac tacggcggtc
    18421 aactggtagc ttgttttgct gttgatcagg atgaaaatcc gcagcgttaa ctcacaaaac
    18481 cggggccatg cgctccggtt tactcaatca gcttcaacag tggcgacaag catagcaaaa
    18541 tgccgtacag cagaatcagc caggctttaa atccgcgaag tttttccagt tgtgcgactt
    18601 tatagatcag gaaaaacgga atgaggcacg acacaatacc atataacggg cttcccaact
    18661 gaaagaacac cagcaccgat acacgaaacg aaacccaaat cgtcaacgta atgacgataa
    18721 aagcgcagat cgccagagtc agcacgcgtg agttaatttt cttggtatca ataattcggc
    18781 ttaacagatt gagaataatg cctttaatgg cctcgtggaa accgagataa atgccaaaga
    18841 atgcggtcag tacggcaaag atattaagca ccgtagaggt gatatgaatg atatgcccag
    18901 ggatcacctg cgcggccagc gccagtgctg agatattttg ttcaaaggca gaaacggctt
    18961 cttcgtggct aattgagaag gtaaacgaaa aggcaaaaaa caggatcacc gcgatgagcg
    19021 tgatataact aattcggtgg gtacgcagcg cgagccgggt tgccagtact ttatccgctt
    19081 cccgtttacg ataggcaata ttcattgggt taagtacctg aataaatact gcagaaaaga
    19141 agcaaaatgg aatggtaagc aagacatcgc ggaaaaagac ggaggcttgc gggaaggcgg
    19201 ttatattggc gaaattccag tgcgggatca tcgcaaaacc gaacacgaca ataatcccta
    19261 ctttgaccac caccattggc ccggaaatct taaacagtaa tcgttcacca ccagacgcaa
    19321 tcgccaccag tacggcgaaa atagcgactt tataaagtag agattgtgaa agatcggcat
    19381 cggttaaacc gaaggttttc aggtacgagg cgctgtcgaa aaccacggag agagagtaga
    19441 taaaaatccc gtggataatc atcaaaaagt agataacccc gaggaaaatt ccccagttct
    19501 tccccaggta atgactgata atatcggtgt agtcattaca ggaatcgctt tcagaaaggg
    19561 tttttaaata aatgtcctgc actacccagg tggcaggata agcaatgatc gctgcggtaa
    19621 taaataccca aattcccttc aagccaattt gtactggcat cagcacggtt ccggcaccaa
    19681 tcgccatgcc tatgcataat aaaacccagc cgaagtcgta gcgtgtaaac ggcaatttct
    19741 gattgtgttt cgatagtgtg ttgtgctgca taaagtcctg tccagaaaga agtcggcctc
    19801 gcaaaaatga ataaacccag tttctcttgc gcaacaggtg gagaagtcga atacgcagta
    19861 tgtaaaaaat gaggcaacag attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa
    19921 ttgcgcattt cactaccagt ctggagtcag cccggatatg aactgcaact attgcaaata
    19981 aagaaattaa gctggaaggc atcgcgcaat gaggaagatt gccgggtgct gacagtaaaa
    20041 agcaatcgta gaatcagcgg caagccggaa aggttccggt gaggcgcaat gttgcggggg
    20101 ctttatccct ggtggcattg gttgctggag agagaaaacc cccgcacgtt gcaggtatgc
    20161 acctgacaac atcacggggg ctaatcttga acccaaacaa ctcaagaata gccgcgaaac
    20221 gttggcatta caacacaggc ggctatatga cgctcgcaga actgggcatg gccttctggc
    20281 atgatttagc ggctccggtc attgctggca ttcttgccag tatgatcgtg aactggctga
    20341 ataagcggaa gtaacgtgtc atgcgggcgt caggctgccg taatggcaat ttgcgcccgg
    20401 accaggccgc aggggtgaaa ctctgcggcc tttttcgtaa gaaaaatgca acggttgcaa
    20461 gcggataata ttctgcggaa tgcgcagcac agagtgaaga tgagacggat tccggaacag
    20521 aaaaacagtc gtagaatcag cgtcaagccg gaaggattcc ggtgaggcgc aatgttgcgg
    20581 gggcttaatc cctggtggca ttggttgctg gagagagaaa acccccgcac gttgcaggta
    20641 tgcacctgac aacaccacgg gggccattct tgcatccaaa caatacaaga atagccgcga
    20701 aacgttgtca ttacaataca ggcggctata tgacgctcgc agaactgggc atggccttct
    20761 ggcatgattt agcggctccg gtcattgctg gtattcttgc cagtatgatc gtgaactggc
    20821 tgaacaagcg gaagtaacgt gccatgcggg cgtcaggctg ccgtaatggc aatttgcgcc
    20881 cggatcaggc cgcaggggtg aaactctgcg gcctttttcg t
//
LOCUS       DAPHBJ010000070        20819 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.070, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000070 DAPHBJ010000000
VERSION     DAPHBJ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.070"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            67..729
                     /gene="atoD"
                     /locus_tag="RHZ76_002289"
     CDS             67..729
                     /gene="atoD"
                     /locus_tag="RHZ76_002289"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HDV2812922.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            729..1379
                     /gene="atoA"
                     /locus_tag="RHZ76_002290"
     CDS             729..1379
                     /gene="atoA"
                     /locus_tag="RHZ76_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HDV2812923.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            1376..2698
                     /locus_tag="RHZ76_002291"
     CDS             1376..2698
                     /locus_tag="RHZ76_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HDV2812924.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            2729..3913
                     /gene="atoB"
                     /locus_tag="RHZ76_002292"
     CDS             2729..3913
                     /gene="atoB"
                     /locus_tag="RHZ76_002292"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HDV2812925.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SQHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTTEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKNLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(3987..4763)
                     /locus_tag="RHZ76_002293"
     CDS             complement(3987..4763)
                     /locus_tag="RHZ76_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HDV2812926.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPIHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4768..6417)
                     /locus_tag="RHZ76_002294"
     CDS             complement(4768..6417)
                     /locus_tag="RHZ76_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HDV2812927.1"
                     /translation="MNWRRIVWLLALVTLPMLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMAAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQAETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(6418..10932)
                     /locus_tag="RHZ76_002295"
     CDS             complement(6418..10932)
                     /locus_tag="RHZ76_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HDV2812928.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPFEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGKELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     IGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVTLNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQAIGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTRDINDSLILDTPEYPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            complement(10956..11579)
                     /locus_tag="RHZ76_002296"
     CDS             complement(10956..11579)
                     /locus_tag="RHZ76_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HDV2812929.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(11576..13264)
                     /locus_tag="RHZ76_002297"
     CDS             complement(11576..13264)
                     /locus_tag="RHZ76_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HDV2812930.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERTAEKTPVRQRIVVSARWLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPARASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGIAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(13413..16040)
                     /gene="gyrA"
                     /locus_tag="RHZ76_002298"
     CDS             complement(13413..16040)
                     /gene="gyrA"
                     /locus_tag="RHZ76_002298"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HDV2812931.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            16187..16909
                     /locus_tag="RHZ76_002299"
     CDS             16187..16909
                     /locus_tag="RHZ76_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HDV2812932.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            complement(17037..20789)
                     /gene="yfaL"
                     /locus_tag="RHZ76_002300"
     CDS             complement(17037..20789)
                     /gene="yfaL"
                     /locus_tag="RHZ76_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HDV2812933.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAVTDSCQGYDVKASCQ
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIVNNTAFTNNTAEGYGGAIYTNSVTAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSSAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQN
                     QAELNVGSTQQTFVHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANN
                     NSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITSDVDTRSDAAGHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILPYASSLWVGDGATFVTGADQDIQSIDAISSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSNVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPTPDPDPTPDPDPTPDPEPTPAYQPVLNAKVGGYLNNLRAANQAF
                     MMERRDHAGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDGEW
                     MLGIVGGYSDNQGDSRSNMTGTCADNQNHGYAVGLTSSWFQHGNQKQGAWLDSWLQYA
                     WFSNDVSEQEDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAA
                     NRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDGSTISDDAV
                     KQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
BASE COUNT         4731 a         5743 c         5263 g         5082 t
ORIGIN      
        1 cattctggca ctccccttgc tattgcctga ctgtacccac aacggtgtat gcaagaggga
       61 taaaaaatga aaacaaaatt gatgacatta caagacgcca ccggcttctt tcgtgacggc
      121 atgaccatca tggtgggcgg atttatgggg attggcactc catcccgcct ggttgaagca
      181 ttactggaat ctggtgttcg cgacctgaca ttgatagcca atgataccgc gtttgttgat
      241 accggcatcg gtccgctcat cgtcaatggt cgagtccgca aagtgattgc ttcacatatc
      301 ggcaccaacc cggaaacagg tcggcgcatg atatctggtg agatggacgt cgttctggtg
      361 ccgcaaggta cgctaatcga gcaaattcgc tgtggtggag ctggacttgg tggttttctc
      421 accccaacgg gtgtcggcac cgtcgtagag gaaggcaaac agacactgac actcgacggt
      481 aaaacctggc tgctcgaacg cccactgcgc gccgacctgg cgctaattcg cgctcatcgt
      541 tgcgacacac ttggcaacct gacctatcaa cttagcgccc gcaactttaa ccccctgata
      601 gcccttgcgg ctgatatcac gctggtagag ccagatgaac tggtcgaaac cggcgagctg
      661 caacctgacc atattgtcac ccctggtgcc gttatcgacc acatcatcgt ttcacaggag
      721 agcaaataat ggatgcgaaa caacgtattg cgcgccgtgt ggcgcaagag cttcgtgatg
      781 gtgacatcgt taacttaggg atcggtttac ccacaatggt cgccaattat ttaccggagg
      841 gtattcatat cactctgcaa tcggaaaacg gcttcctcgg tttaggcccg gtcacgacag
      901 cgcatccaga tctggtgaac gctggcgggc aaccgtgcgg tgttttaccc ggtgcagcca
      961 tgtttgatag cgccatgtca tttgcgctaa tccgtggcgg tcatattgat gcctgcgtgc
     1021 tcggcggttt gcaagtagac gaagaagcaa acctcgcgaa ctgggtagtg cctgggaaaa
     1081 tggtgcccgg tatgggtggc gcgatggatc tggtgaccgg gtcgcgcaaa gtgatcatcg
     1141 ccatggaaca ttgcgccaaa gatggttcag caaaaatttt gcgccgctgc accatgccac
     1201 tcactgcgca acatgcggtg catatgctgg ttactgaact ggctgtcttt cgttttattg
     1261 acggcaaaat gtggctcacc gaaattgccg acgggtgtga tttagccacc gtgcgtgcca
     1321 aaacagaagc tcggtttgaa gtcgccgccg atctgaatac gcaacggggt gatttatgat
     1381 tggtcgcata tcgcgtttta tgacgcgttt tgtcagccgg tggcttcccg atccactgat
     1441 ctttgccatg ttgctgacat tgctaacatt cgtgatcgcg ctttggttaa caccacaaac
     1501 gccgatcagc atggtgaaaa tgtggggtga cggtttctgg aacttgctgg cgtttggtat
     1561 gcagatggcg cttatcatcg ttaccggtca tgcccttgcc agctctgctc cggtgaaaag
     1621 tttgctgcgt actgccgcct ccgccgcaaa gacgcccgta cagggcgtca tgctggtcac
     1681 tttcttcggt tcagtcgctt gtgtcatcaa ctggggattt ggtttggttg tcggcgcaat
     1741 gtttgcccgt gaagtcgccc ggcgagtccc cggttctgat tatccgttgc tcattgcctg
     1801 cgcctacatt ggttttctca cctggggtgg cggcttctct ggatcaatgc ctctgttggc
     1861 tgcaacaccg ggcaacccgg ttgagcatat cgccgggctg atcccggtgg gcgatactct
     1921 gttcagtggt tttaacattt tcatcactgt ggcgttgatt gtggtgatgc catttatcac
     1981 ccgcatgatg atgccaaaac cgtctgacgt ggtgagtatc gatccaaaac tactcatgga
     2041 agaggctgat tttcaaaagc agctaccgaa agatgctcca ccgtccgagc gactggaaga
     2101 aagccgcatt ctgacgttga tcatcggcgc actcggtatc gcttacctgg cgatgtactt
     2161 cagcgaacat ggcttcaaca tcaccatcaa taccgtcaat ctgatgttta tgattgcggg
     2221 tctgctgcta cataaaacgc caatggctta tatgcgtgct atcagcgcgg cagcacgcag
     2281 taccgccggt attctggtgc aattcccctt ctacgctggg atccaactga tgatggagca
     2341 ttccggtctg ggcggactca ttaccgaatt cttcatcaat gttgcaaaca aagacacctt
     2401 cccggtaatg acctttttta gttctgcact gattaacttc gccgttccgt ctggcggcgg
     2461 tcactgggtt attcagggac ctttcgtgat acccgcagcc caggcgctgg gcgctgatct
     2521 cggtaaatcg gtaatggcga tcgcctacgg cgagcaatgg atgaacatgg cacaaccatt
     2581 ctgggcactg ccagcactgg caatcgccgg actcggtgtc cgcgacatca tgggctactg
     2641 catcactgcc ctgctcttct ccggtgtcat tttcgtcatt ggtttaacgc tgttctgacg
     2701 gcaaccctac aaacagaagg aatataaaat gaaaaattgt gtcatcgtca gtgcggtacg
     2761 tactgctatc ggtagtttta acggttcact cgcttccacc agcgccatcg acctgggggc
     2821 gacagtaatt aaagccgcca ttgaacgtgc aaaaatcgat tcacaacacg ttgatgaagt
     2881 gattatgggt aacgtgttgc aagccgggct ggggcaaaat ccggcgcgtc aggcactgtt
     2941 aaaaagcggg ctggcagaaa cggtgtgcgg attcacggtc aataaagtat gtggttcggg
     3001 tcttaaaagt gtggcgcttg ccgcccaggc aattcaggca ggtcaggcgc aaagcattgt
     3061 ggcggggggt atggaaaata tgagtttagc gccctactta ctcgatgcaa aagcacgctc
     3121 tggttatcgt cttggagacg gacaggttta tgacgtaatc ctgcgcgatg gtctgatgtg
     3181 cgccacccat ggttatcata tggggattac cgccgagaac gtggccaaag agtacggaat
     3241 tacccgagaa atgcaggatg aactggcgct acattcacag cgtaaagcgg cagccgcaat
     3301 tgagtccggt gcttttacag ccgaaatcgt cccggtaaat gttgtcactc ggaagaaaac
     3361 cttcgtcttc agtcaagacg aattcccgaa agcggattct acgactgaag cgttaggcgc
     3421 attgcgcccg gccttcgata aagcaggaac agttaccgcc gggaacgcgt ctggtattaa
     3481 cgacggtgct gccgctctgg tgattatgga agaatctgcg gcgctggcag caggccttac
     3541 ccccctggct cgcattaaaa gttatgccag cggtggcgtg ccccccgcat tgatgggtat
     3601 ggggccagta cctgccacgc aaaaagcgtt acaactggcg gggctgcaac tggcggatat
     3661 tgatctcatt gaggctaatg aagcatttgc tgcacagttc cttgccgttg ggaaaaacct
     3721 gggctttgat tctgagaaag tgaatgtcaa cggcggggcc atcgcgctcg ggcatcctat
     3781 cggtgccagt ggtgctcgta ttctggtcac actattacat gccatgcagg cacgcgataa
     3841 aacgctgggg ctggcaacac tgtgcattgg cggcggccag ggaattgcga tggtgattga
     3901 acggttgaat taatcaataa aaacacccga tagcgaaagt tatcgggtgt tttcttgaac
     3961 atcgacggcg aaggtaaccc cattaatcac cagtcaaaac ttttcaccag cgtcagctcg
     4021 ccagcattac gcatcggtac aataaatgtt tcctgtttct cattgaccga tccttcatcg
     4081 gtgatcagcg tcagttgggc ggtggttaat tccgtttcgc tgcgcccacc atagtagttg
     4141 atatacacct gatagcgccc gtgaattggc gcgggcatgg cgaaaatctc gggtccgtac
     4201 cccgtcgtga catccatatc cagtgcacca ctgtttttca gcacggtgtt accgtaccag
     4261 gcgtgttcgc catcgggcgt aacaacgtga aggtcgaggt cggtattgtc cgtatcccac
     4321 gagagaacca gccgtaaacg tgcacgaatc gttcctgtac ccggcgttga gtaaaactgc
     4381 attttttgtc ggctttgccc atccgggctg atgacctgca cgctgttgct gccttcagtg
     4441 aaaatataag ggcgtgcaaa tgaaccatcg gattcaattc tctgcggcat actggcaccg
     4501 ttgaccacca gccggccctg ctgaacttta cccgccgcag cataattctt tattttgccg
     4561 cggatctgcg ctgagatatt ttgatcatct gccatgttga cggacgacgc cgggtaattg
     4621 atcgattgtg aaaaactggc atcttcgccc tctgccggat gccagcctga gagcggtgca
     4681 tcaatctcga cttcctgcac accttcactg tgagcgacag gcgaaagcgc caccagtaac
     4741 aacggaagaa aaatctttcg catagcatta gtccattaat aattgtcggg tcagcgtttc
     4801 gatatagttc tcatcaagcc cggtgggata accatcaaag gccaggtgca gatactcatg
     4861 ggttaaatcc agccgatcct gaagcgtaaa gaagttacga ataaacagcc gtttctgctg
     4921 ccgatcggta taggggaaac ctgagactaa acggcaaacc gcaaacacgt ctggttcgtt
     4981 gtaccccgtc tcagcttgta atatacgccg ccactgcggc atttttttcg ccagccaggc
     5041 tttcgctttg ggtaataact ggcaggttga gcgtggcgct ccccagcggc taaggctgtt
     5101 gtcgggataa gcaaacgcga gtatctggtc gtaacgctct ccctgccccg cctgggccgt
     5161 ggcttgccgc caggaaagcg tcccttcggt agcgcgactg ccatgataat gaaccggatc
     5221 gcccgcatag atgagatcct gcgtccaggc ggccatcgtt cgcgcccccg tcgtagctgg
     5281 cgaagccgaa acacgctgcg tggcgctgct gtcagggatg gtcaggcaat cgccttcccg
     5341 attggcgttt tgctgtaaaa aagtgcgaat cgccacggtc atggctttcg ctgcctcagg
     5401 gggcgtactt ttcgcctcgc gatccagaac tctggcaacg tactcttcac gatcgagatg
     5461 tgactgcaat ttcagtttgc ccttttcgct cagtaaggtc gtctcaccgt ggctgacaaa
     5521 cgtaatgtgg ttaccattag taaacgtcac acgatagcgc ccattaagta cgccgggttt
     5581 tactgccgtc gtactctttt ccgctgtgat tttcttcagc ggatagcggg caaacagctc
     5641 cacctctaca cattgcccac tggcaacctg cgtcggcact ggcaaaaccc gattaagcac
     5701 cgtcgcataa cgggttagta ccgttttgct ggtgccactg ccagtaaccc acagcggtgt
     5761 accatcggtt aaccagccag caaaaccacc ctggcggatt tcctgtttat catcggcaaa
     5821 ccagctccag gttttcaccc gtacccgact accgagcatg gatgcgacac cgattttcgc
     5881 ctcgtccagc accacatcca gtaagacttc ctgagctttg ttttgcgcag gtaacgtcgc
     5941 taatgaatcg agcagcgatt taaccgttac cgaggtttct ggcttcagcg ttgtaagaga
     6001 cgctaaccac gcaggtgcct gacgctgttg ccagtattgc ccccacacat ctgcgccaat
     6061 atgcaaccgc tgcggcgcaa aatagagccc acaggaacgt accagcgcgg tatcgcgcgt
     6121 tatgctctcc ccagcctgac aacaataaac ctcttccggg gagtttccgc gacactggta
     6181 gggctgctcc ggttgatggg tatcctccag ccaggcgtaa acatacagtt tccacaaact
     6241 ccctaacggc gtggtgagcg agtcaggtaa tgctgagact ttggtcacgc ctgatgacga
     6301 cagctgatac agttgatcgt gctgcgcacc gcgcagcacc agttgtaacg gcgtctcctc
     6361 cgcaagcatt ggcagagtaa ccaacgccaa tagccagacg attctgcgcc agttcactta
     6421 tttcacctgc atcccggtcc attcactccc tgccgcaaca ctttgctgcg caggtgcata
     6481 ggaacgcaca taacgagcag gaggcagaac gaattgccct ttttgcgaga agcgcagcaa
     6541 atggcggaaa gtgaccgttc ccgtcagttc tttcaccggc accatatagg ccagttcgcc
     6601 catttcatta cgtgcttttt ccagcaattg cccctgttgt ttcgcggcgt tgggtttatt
     6661 gaccgaaatg ccccatgttg tgcgctcaac gtcggctccc ggtgggagcg gtacttccac
     6721 ctgaccgtag cgcagaactg catcctgctc gctggtaagc gtgatttcat cgagatacag
     6781 cgcatcgctg tcaatctcat tgctggtcac cggttgcaga gtaaagctca tctcttcttc
     6841 accagggata agccgataca actggcgttc aacggttacc ggaatgttac tttgttgagc
     6901 cgtttttgcc ggttcacgcc agcggacctg cacattttgc ggcgataatt cgtcaccaaa
     6961 agagagaatg tccggcacgc cctgaccaac ccaacgccag tcttcgcccc ctccagttaa
     7021 cttatgttta gcccatgcgc ccgcaggcgc aggcaaaaca actggaggca ttgtcgccat
     7081 atatttcgcc agccagttca tggccagcgc gcgctcaata gtggattgct cagcggtcaa
     7141 accacttaaa atagcggccg tctgggtagc gtcgccaccg ctattaagca gcacaaccgt
     7201 gtgcgccagc ggctgattgc tgtttgccgc tttatcccac gcatattgct gaacctgtgg
     7261 catcaccgtg gacttcaact gcgctttttt cagcaacgtc atggttaaga cgtttgccac
     7321 cgcatctgcc agtggatact ccggtgtatc gaggatcagg ctatcattga tatcgcgggt
     7381 gtcttcctca gagaaatctt cagttttagt tccgcgccgg gcgatagctt catccaaccc
     7441 tttcaacaac gttttgcacg gcaaattcat ctcctgcgcc catgccagca ccagcgcccg
     7501 atgcaataac ggcatgttat ctgcctgctc cgcgtagctg tcgagcatat gctgccagta
     7561 ttccggttgt tgcgttacgc cgatcgcctg gctggcctgc cagtcggcgt accatgccca
     7621 tgccgtaagg aaggcgtcac cattgccatc ttcaccccac caggtaaagc gcgctccggg
     7681 ccccgccagt tgcatcagcc gcagacggtt atcctgaatc atctgacgaa tgtcgttagc
     7741 ggcggcactt tgatgatcgg caagcgaacg ccaggcgaga ctgagcggga tcagacggct
     7801 accggtgttg attacgccac cccacggttc atcgactaac gcatcaagat tgttgcggaa
     7861 aatctcctgc ggcgtttcac tactttgtag ccggatattg ctcgcctgct cgggcaacat
     7921 cagcgcgtta tcgccaccac cgagcatgac atttttctgc tgttcaacgg gccagctgtt
     7981 atccacaaaa gacagttttg tgctaatgct gtcctgcact tgcccatttt gttgcagttc
     8041 tgcacttaac aagccagatt gctgaatatt ctgcgtcagc gaaatataat tcgccccttt
     8101 gtgcagcgtc agcgtctggc gcatctcagc gcctgcaaat ttagtcacca gcgccaccgg
     8161 ttcgttatcc tgctgactga agataaacag tcctgccgcc ggtttgtcgc ccacgcgata
     8221 caccgttggc atactccact tcatgtagag atttttttcc gaacgcagat aagcacgccc
     8281 ctgcccgacc agcccgtcgc cgttcatccc acgcgcggtg atacgccagc gggttaacga
     8341 atcaggcatc aggaacgtga aatacgcttt gccttgttta tcggttgtga gtgacggcat
     8401 ccatgccgcg gtatccacct cttcacgccg tggacgttca agcattttta ctcgccgctc
     8461 gctgcggtta gttgcgccag gcgcaaccgg ctcgctggag agcgcctggt cgtagctgat
     8521 aaacgacaga ctggagctgg tacgcacatt gttacgcccc agcggataga aaaatttgcc
     8581 gatattcggc gcgatttctg gttgcagcgc gtagatcatt tcatcgacca cgcctaccgt
     8641 tagctgcgca gaaacaggtt tacctttcag cgacgaggtt aattcgacat tgaccagttc
     8701 accaggctgg taatgggttt tgtccgtttt cacccggata tccagctggg gaacggcaac
     8761 tttgatcccg gcgttctgaa aactgtactg accgttacgg gtatacagca ccgaaaaagt
     8821 gatgttaggc gcaaaggaat tgctcactgg aacccgggct tcatactggg tatcgtttaa
     8881 acgttgtagc gttagccagt ttgccggatg cgaaagcagc gactgctgtt ccacgcgatc
     8941 gcgttccagc gtcaataatg cttcatcaat tggctccgga aaggtaatca gcatcttcgc
     9001 ggtttcgcct ggctggtaca gcgttttatc cgccacgata tctaccgtac cagtatgcgc
     9061 cgtgctgccc ttaccgctga cggcatgact taacccagcg agaattaagc cgtctttatc
     9121 gcgtaatgtc agattgtagt tgccaggttt agcgaaattg acggtaaagg atttgccgcc
     9181 tgacggtagc tctccgctat ggctcgtgcg gtcttcgaga cgcaaccatt catacgtaac
     9241 aggaacctgt tttgaagatt ccagcgcggc ataacggaac acaaccgact cgccgctatt
     9301 actgtattgt gcggcggtac ttaaagagta atgcgccagg ccgcgttcaa tgaggatctc
     9361 tttggtggtg gtgacgcgat acgccgcgcc gtcactggcg gagacggtta acaaatagcg
     9421 gctcggttta tcggcggcgg ggagatttaa cgtcacatga ccgctggcgt cggacaccgt
     9481 ttcgctgcct tccagcgaca cggggaaacg tccggcataa cgcaaatcgt taccgaccat
     9541 tgataattgc tgagcgcgca aacttaactg cacgcgggca ttttttaccg gctcgccatc
     9601 cgggtagagc agttgcagtt tgccgctgac cgcttcgcca gttttgaact cttttttgtc
     9661 gagagctaaa ccaatctcga aatgtggctt gatgtagttt gccacgcgaa aactgctgct
     9721 atagacctga ttgcggtatg caagacgtaa ctcataacct ccggctacgg cattttctgg
     9781 caggcggaaa cttccctgcc cgccattgcg cgcatccagc gtgacattga cggtttgcaa
     9841 cagactgccg ttggcgtcca gcaccgaaag cttcgccggg gcgctgacga tgggggatga
     9901 atgcaacgga tcgtggaact cgcggccgat cactttaaca tcgacacgat cgcctgcgcg
     9961 atatagcggg cgatcggtaa aaatatacaa gcgggtgttg tagatttcgc tttcgtagaa
    10021 gaagttctcg gagacaaaaa cgccgccttc agcatcctta cccagaatgt atgaacgttc
    10081 tggcgatata tgttgtaact gcaaggtacc gctgtcatcg gtcacaccgc gggtcatcac
    10141 gccaagaccg tcagtccaca agatctctga gccgggcttc gcttcaccct gttttttacc
    10201 cgcggtccac accagaagct ctttgcctga cactttgcta agcgccacgg tatcggaaac
    10261 aaacaccacc gtcgtcgccc gatacccacc aaccatcgcc tcgacgaggt acagtcccgg
    10321 ctcttgttgg ccgagaggaa tataaatgtt acccggctgc ggcgagatga aattgctgga
    10381 tgcgccttcc agttttaccc cttgctgcgg ctcgaacggt ttagcctgcc atagtggata
    10441 acggaactgt tccaccaggg gatatttttt cagcggggaa aactggttgt tctgtacata
    10501 acgggaaggt ttaataatgg cattgccgag ctgtaattcg ggtaacgcct gagtcacatt
    10561 ctgccgtgac tgagaagaga aagtacgctg catcacgcgg cgagatttgc cgtaccagtt
    10621 atcccacagc caggttagcg tattgttcag cccgtcgccc agatattgcg gttgcaccac
    10681 aatgcgatgc aggtttttct gctggcgcaa aaatgccatc gggtcaggaa tacgatacag
    10741 gcgaacgtcc acgccgccgt actcttccat ctgatagcgc cgataatcac gccccggcgc
    10801 ttccagtcgc actttcgcct cttcactgct gctaaaactg ctgtcagcaa gcaaaaagaa
    10861 tgttcccccg gcaggcggcg catagttgct ggaaggaagc gaatcatcag cattagcaag
    10921 ccctgttccc acaagagaca gggacaagca cgctgctatc gcgccagaaa atttaaacga
    10981 taaatgccaa tgaaattggg attggaatcg ttgggtatcc atcgggtgtc cttccatgtc
    11041 ataagttgtt gcagactgac tgcgcgcatt ccgttgtcag ttttcgtggc gcttccggtg
    11101 tggtagatga cgtaacgccc catccagacc attaagtgct gggcatcgcc ctgatcgaaa
    11161 aaaatcatat cgccaggcag cgcctggttt atgtcctggc caataaactg gctgttgtac
    11221 tgaatcaaat taatcgcggt cacatagggg ccggttttcc cgttcccctg attccagttt
    11281 tgcgccagtt gacgctgttc aggtgttagc gtcatctctg gcggcaaata ctggctagat
    11341 aaaccgttac tttttagcca tttactgtcg tgaactttca gcgtctcgtt cgccgcaaat
    11401 cgcaccaggc ccgcacaatc ctgctgatac cagcgtggac ttggcccctg gcggagctgt
    11461 tcttgtgcaa tgcgtacaaa ccaggcgcga aacagcccgg attgttcgac attcagcatt
    11521 tcgctatggg caacaacaca acacagccag caaatcagcg ccagcagccc gtgcctcata
    11581 gcggctgcca ggttatgggt agccactgcc aggcggcacc gggttccatc tgggccagct
    11641 tcatgacata acgcggttgt tgagataaag cgtccagctt cggcattaat aaagtttgtg
    11701 cggcgttata aaaaaccggt tcgagattct tcggcagact ggtcaacgtt tcgttacgca
    11761 gcagtttcgc tattccttgt ggattgatat agagcggaac gatgccatca gtgggtatta
    11821 catccaccat tgccgggcgg gttttattca gtgtttgcag cgcgttatta accagcgtgt
    11881 catcgaggga gaaaagcagc gttttgtttt gcatcgccag cgacacacgg aaaaaataat
    11941 ccgacattaa ttgatcgggt tgcgccgcct gcgctttcgg atactggccg tatcgggaac
    12001 tcacttcgcg acgccagatt tgcgcttcgc cctgctgagt ctggcttacc ggcaaaacac
    12061 cttctggcgc tttgctttcg tgcgcaccaa tattttgcgt aaacagtttc cctggcaatt
    12121 gcgcctgttc ggcagtgcca tcaaactgac cgacaaacag cggggtttgc aattttgagt
    12181 cttcatacca gcacagcccc gcggcaccgt ctaacgcccc attcagcttg tcgttttcct
    12241 ggctgatgtg cgaaagcatc tcttcggcaa taccgtgtga atacggcacc gccacacaga
    12301 agctggctct ggcaggcata ctgttccata ccggcgtaaa atcgaaactg gcatctacgc
    12361 tggcggattc atcatttaac gccacaaaac tgtgccagcc gtcgttaccc atttcgaagc
    12421 gtacgccagc aaaagaaggc attaaccgct ggtagccaaa ccccagccac ctggcgctga
    12481 ctacgatgcg ctggcgtact ggcgtttttt cggcagtacg ctcttccagg ccaaagcttg
    12541 cttgccagcg ttttttgccg ctcaacaaat cacctgcgat cgccgtggct tcggtatcct
    12601 gctgatcatc tttaaacaac atatccgtgc tggaaaacac cagcatcttg tcctgataag
    12661 tcgcgaacat cagggcgtta ttgccgttat agcgcaactg ataaacagga acagtttcac
    12721 tatttatctt gatgctactg atttccgttt tgcttaactg gctgtcgctg gtagcggcaa
    12781 acaacaatgg ctccagcagt ttgcttaaac cactgcgctg gatcagcacc atataatgtg
    12841 aaagatggcc ttgtttatcg tgccacagcg ctgcctgcgc gggctgatct aagagtgacg
    12901 aaaagagctt atctttcagc gtgagatcgt gttcatagac aatacgacga atgctgcctt
    12961 caatgcccag acgatcggca tgattctgat aatagaaaac gaaatcttcg ctcagaacat
    13021 cgtggagaaa cggaatggtg aggagatctt tgggaagctg gctcagagag tcgctgtcga
    13081 gaaagaggtc cggctcattg agatcgattt gcagattgtt gtgcaccacc agcggcgaca
    13141 acgttttttc tggcccactg ccagcatatt gcaacgccca gacgccagcg gaaagcagtg
    13201 ctattgcgcc aaaacctaca agaccataga accgccagcc tttcgccttt ttttcaccac
    13261 tcattgccac attccttgtg tatagccagc cattttttac gggcacagcc aaactttacc
    13321 gtgccctaat acgacaaaag cccagacttt gcagcctgga cttttcaatt caaacaaggg
    13381 agatagctcc cttttggcat gaagaagtaa aattattctt cttctggctc gtcgtcaacg
    13441 tccacttccg gagcgatttc atcgtcccct tccgcggcac tgccgtcgat ggtatccaga
    13501 tcttcctcgt caaccggttc agcaacacgt tgcagaccca ctacgttttc atcttccgca
    13561 gtacggatga ggatcacgcc ctgggtgtta cggcccacga tgctgatttc cgaaacgcga
    13621 gtacgtacca gcgtaccggc atcggtgatc atcatgatct ggtcgcagtc atctacctgt
    13681 accgcgccaa caactaaacc gttacgttcg gtaaccttga tggagataac ccctttcgtc
    13741 gcacgcgact tggttgggta ttccgccact gcggtacgtt taccgtaacc gttttgcgtt
    13801 gcggtgagga ttgcgccatc gccacgaggc acgatcagag agacgacttt atcgccttca
    13861 cctaagcgaa taccgcgaac accggtggtg ttgcagccca tcgcacggac agaagactct
    13921 ttaaagcgca ccactttacc ttcagcggag aacagcatta cttcgtcttc gccgctggtc
    13981 aggtcaacgc cgatcagctc atcgccgtca accagtttga tcgccacttt accggcggta
    14041 cgcagacggt tgaactcggt gaggacagtt ttcttcacgg taccgttagc ggtcgccatg
    14101 aagactttca cgccttcttc aaactcggtc actggcagga tcgcagtgat acgttcgtcc
    14161 tgctccagcg gcagcaggtt gacgatcgga cgaccgcgcg cgccacgagt ggcttccggc
    14221 aactgataaa ctttcatcga atagacgcga ccacggctgg agaagcacag aatatggtcg
    14281 tgagtgttcg ccaccagcag tcggtcgata aagtcttctt ctttaatacg tgcggcagat
    14341 ttacctttcc cgccacgacg ctgcgcttcg tattcagaaa gcggctgata cttaacgtag
    14401 ccctggtgag agagcgtcac taccacatct tcctgagtga tcagatcttc caggttgatg
    14461 tctgcgctgt tggcggtgat ttcagtacga cgtttgtcac cgaactgttc acgaaccagc
    14521 tccaactctt cacggatcac ttccatcaaa cgatcggcgc taccaagaat acgcaacagt
    14581 tccgcgatct gatccagcag ctctttgtat tcgtcgagca gtttttcgtg ctcaaggccg
    14641 gtcagtttct gcaaacgcag atccagaatc gcctgagctt gctgttcggt caggtagtac
    14701 agaccatcac gcacgccgaa ctctggctcc agccattccg gacgcgcagc atcgtcgcca
    14761 gcacgttcga gcatcgcggc aacgttgccc agctgccacg gattagcaac cagcgcagtt
    14821 ttcgcttctg caggcgtcgg cgcatgacgg atcagttcga tgatcgggtc gatgttcgcc
    14881 agcgccacgg ctaatgcttc aaggatatga gcacgatcgc gagctttacg cagttcgaaa
    14941 atagtacgac gggtcaccac ttcacggcgg tgacgaacaa acgccgcgat gatgtctttc
    15001 aggttcatga tcttcggctg accatggtgc aatgccacca tgttgatacc gaaagaaacc
    15061 tgcaactggg tctgggagta gaggttgttg agcacaactt caccgaccgc atcgcgtttc
    15121 acttcaatca cgatgcgcat accgtctttg tcagactcgt cacgcagcgc gctgatgcct
    15181 tccacgcgtt tttcttttac cagttccgca atcttctcga tcaggcgcgc tttgtttacc
    15241 tgatacggaa tttcgtggac gataatggtt tcacgaccgg ttttggcgtc aacttccact
    15301 tctgcgcgag cgcggatata caccttgccg cgaccggtac ggtaagcttc ttcaataccg
    15361 cgacgaccgt taatgattgc cgccgtcggg aagtccggcc ccgggatgtg ttccatcagc
    15421 ccttcaatgc tgatgtcttc atcatcgata tacgccagac aaccgttgat gacttccgtc
    15481 aggttgtgcg gcgggatgtt ggttgccata cctacggcga taccggaaga accgttcacc
    15541 agcaggttag gaattttggt tggcatgacg tcgggaattt tttccgtgcc gtcatagtta
    15601 tcaacgaaat cgaccgtctc tttttcgaga tcggccatca gttcatgggc aattttcgcc
    15661 agacggattt ccgtataacg cattgccgcc gcagagtcgc cgtcgatgga accgaagtta
    15721 ccctgaccgt ctaccagcat gtaacgcagc gagaatggct gcgccatacg gacgatcgtg
    15781 tcataaaccg ccgagtcacc atggggatgg tatttaccga ttacgtcacc aacgacacgg
    15841 gcagattttt tataggcttt gttccagtca ttgcctagta cgttcatggc gtaaagtacg
    15901 cgacggtgta ccggcttcag gccatctcgg acatctggca gcgcacggcc aacaatgacc
    15961 gacatcgcat aatccagata ggagctcttc agctcttcct caatgttgac cggtgtaatt
    16021 tctctcgcaa ggtcgctcat ctaaccgcta tccctctact gtatcccgga ttcaaaggtc
    16081 gcaaattata acacagccgc gcagtttgag gtaaacctat acgctttatt cacatccaat
    16141 gcctgatata ctcgtttgtc ttgccaatta cggagtagaa gtgccaatga atgccgaaaa
    16201 atcgccggaa aaccataacg tagaccacga agagatcgct aaatttgaag ccgtcgcctc
    16261 ccgctggtgg gatcttgaag gtgagttcaa accgttgcac cgcattaacc cgctgcgtct
    16321 gggctatatt gccgagcgtg ctggcggttt atttggcaaa aaggtgctcg atgtcggttg
    16381 tggcggcggc attctggccg agagtatggc gcgcgaaggc gcgacggtga ccggtctgga
    16441 tatgggcttt gagccattgc aggtggcaaa actgcacgcg ctggaaagcg gcattcaggt
    16501 ggattatgtg caggaaaccg tggaagagca cgcggcaaaa catgccgggc agtatgatgt
    16561 ggtgacctgc atggagatgc tggagcacgt ccccgatccg cagtcagtgg tcagagcctg
    16621 tgcgcaactg gtgaaaccag gcggcgatgt ctttttctcg acacttaacc gcaacggcaa
    16681 gtcatggctg atggcggtgg ttggtgcgga atatattttg cgcatggtgc ccaaaggcac
    16741 gcatgatgtg aagaagttta ttaaaccggc agaattgctg ggctgggtgg atcagaccag
    16801 tttaaaagag cggcatatca ctgggctgca ttacaacccg atcactaata cttttaaact
    16861 cggccccggc gtggatgtga actatatgct gcacacgcag aataagtgag gttgatgttt
    16921 ggccgcgcca atgcctgatg cgacgcttgc cgcgtcttat caggcctaca aatgctcccc
    16981 gtaggccgga taaggcgttt acgccgcatc cggcaaccgt gccgactaga cagtgattac
    17041 catttcaccg tcatcgacaa aaaccctgcc gtctgggcaa aatcatcact ccctttctgc
    17101 cacgccacgc tgccgcgcag ggacactcgc tgactgatat tgcccgtgac tcccactttt
    17161 atttcacccc gttgcttcac cgcatcgtca ctgatagtgc tgccatcctc ttcaatttcc
    17221 gtcgaatggg gatcgtgata ataattcaga tctaatgttg gtatgacatg aacagcggta
    17281 cgccattcgc tgtgtaaacc cagccgcgtc tgaatatcat caccctgcga ttgtgacacg
    17341 cgcgcacggt tagcggcggt aaaatcatcc tgctgcacgc cctgataaat cacctgcgcc
    17401 tgcggttcaa tcaccacacc acgccccggt aaccactgat accccgcctc cagcgaggcg
    17461 ataatccccg acgagtggta atgatctgtg ccatcttctt gttcggaaac atcattgcta
    17521 aaccacgcgt attgcagcca gctatccagc caggcccctt gcttctgatt accgtgctga
    17581 aaccagcttg atgtcagccc aacggcataa ccgtggttct ggttatcggc gcaagttccg
    17641 gtcatattcg agcggctgtc gccctggtta tcgctgtagc caccaacaat cccaagcatc
    17701 cactcgccat ccgtgcccca gcgcccgcta aacagatcgc cgctaagttg caccgtagaa
    17761 gtgtcttcat gttgagccag ttgccccgct gctgtgtaat gataatctcc gccgataaca
    17821 cgtaaattca gcgtctgacc atcgccacct gcgtgatcgc gtcgctccat cataaacgcc
    17881 tgatttgccg cccgcaggtt attgagataa ccgccaactt tggcattcaa caccggctgg
    17941 taagcaggcg taggttccgg gtcgggtgtt ggatcaggat ccggcgtggg atcaggatcg
    18001 ggagtcgggt ctggatcagg tggcgatggc ggcgttactt cttgcgatcg cagataccag
    18061 tcgttgttat cttccaccag cgtgtagtca tacgctccca tattgacgta gccgctgcct
    18121 gccagactga actgcgcatt gttttgaaac tgcgtgggat cagctgcgaa atcaaccact
    18181 ttaatgcctg tcgatgtcgg ctcaccaatc cctgtaatgg agttaaccac cacagttgtg
    18241 ttgccagcag tattaccgtt catcaccaat tgatcgctta ccgagtcatc gccgtttaat
    18301 tcgctatcga gcagtagcgt accgccgccg gtataatcac cgtttaccgt cagcgtatcg
    18361 ccagcgacgc cattttgcag gctaaccgca ccggacgtat tggtcagatt gccattaacc
    18421 gtcacgttgg aaagataagt caggctgtca gtttcaaggt tggtattaag ctcacctgtc
    18481 aacgtcacac catcggtggc attcaccagc gtcccatcgc cgttaaatag tgaggcatta
    18541 agggcgacgg aagtatcctg cccggtcagg cgcaaaaccg taccatcgct gatgtcgata
    18601 gtgccgctgg aaatagcatc aattgactga atatcctgat ccgcgccagt aacgaacgtt
    18661 gccccatcac caacccacag tgacgaagca taaggaagga tatcagcaac atcacctttc
    18721 agggtgcctt cttcgacacg taccgccgac tgcgttgtac cgctggcggt cagctccagt
    18781 gtaccagccc ccgttttagt caatgtgcta ccctcatcct gatgctgccc gctgctgtca
    18841 gccatcagtg cgccccactg cgtgtctacc cccgcatcaa ctgccacttc accgtcggcg
    18901 cgcatttcaa tatcacgtcc gtggcctgcc gcatctgaac gcgtgtcaac gtcggaggta
    18961 atctccatca cgctttcttg ttgcttatcg gtaaagataa cctggcggtt atagtgggta
    19021 tctccaagct gcgagttgtc gctcaccatc agcgtaccgg aggcgatttg tgttgtgccc
    19081 aaataactat tattgtttgc cagacttacc tgcccgtcgc tcgcatcgac caccacgtag
    19141 acgccatctt tcccgtcgcc gccgatatgc aaattgtcgc cacctgcaag atctaccaca
    19201 tcctgactgc cgataaccgt tccgctgctg ccactgactt caaggccatc gttgtatgat
    19261 gtgccgctct gccaggtgga gaaatcagag agatcgagca caccgccgtt taacacgatc
    19321 gactgcggat cgtcctggag agcggtaaga tctgccgcgt cgccttccag cgaaagcacc
    19381 gcaccatcat caacgactat atcgccggtt agcgccatcg actgcgcccc tgccagcacg
    19441 tagctgccgt tttgcgcaat ggtgagctga ccagcacctt cgatgatgcc agcaaaactg
    19501 ccctgattaa cagtaacgtt gccaccagca tcgatattta aagtgccatt ctgaaagccc
    19561 gtcaatgcgt gcacaaaagt ttgttgggtc gagccaacgt ttagctcagc ctgattctga
    19621 tactgatcaa tactccctat cgtcagaccg tagcagtctt gcggatcgtc ctggcaatgc
    19681 gtatcgccga cattcatcag ggagttgctg cggcccaggg taacttcacc gttttcaatc
    19741 tgcatctcac cagtaaagtc attgttatct gcattaagta ccagatcgcc ggaacctgtt
    19801 ttggtgatta acccggtacc agcaatagag tcaacagctc cgtcattctc tgtattgcca
    19861 ataaccagcg tttttccgtc ggcaatatca aaggtaactt cacttaagcc gagatacata
    19921 aagccacccg ccgcagagga aggaccatct ccatagcctg ctgcgctatt gttctcatcg
    19981 actaacacgc ctccgttctg gctgtagctg tcatcaacag aaatatcaat aagataggga
    20041 gccgtcacgc tattggtata tatcgccccg ccataacctt cggcagtgtt atttgtaaag
    20101 gcagtgttat ttactatcgt ataacctgaa ggatgcttgc tgtcgctatt attatcggta
    20161 acatcgattg ccccgccatc gccatcactg taacttgttg atgtatatgc ctggttgtta
    20221 tcaaaaataa catcacttaa ataaacgtca ttattaatgg tataaattgc gccacctttg
    20281 ccatcattag cgatgttatt gcgaaacatg gcgttagtga cacgtaaatc gacggcacca
    20341 gtatcgttag taccagaaga atagattgcg ccaccatagc cgcctgcgac gttaccggaa
    20401 aaaataacat cagtaagatt cagcgttgag ttttctttag caaatatggc cccgccgtta
    20461 ttatattctc ctgtaacggt gttattggca aacagagtca ttccagtttc attttctggt
    20521 aatagtgaaa attccgctcc ttgttgcaaa aatacggccc caccgctggc gttattggtc
    20581 atatccgaaa aaaccagcca ttgcccatca gcgatactcc agtcctgcgt aatgcctgaa
    20641 aggctttgcc tgctggcctg acaactcgct ttgacatcat atccctggca tgaatcagtg
    20701 accgccgcga caccgttagc agagaaaaga gatgcaatca ttgacgggag taaagataaa
    20761 tactccttgc gtagaaagat aatccgcata ttaatctaac catcattttc tataagacg
//
LOCUS       DAPHBJ010000071        20740 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.071, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000071 DAPHBJ010000000
VERSION     DAPHBJ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.071"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(67..330)
                     /locus_tag="RHZ76_002301"
     CDS             complement(67..330)
                     /locus_tag="RHZ76_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812934.1"
                     /translation="MMINEQGIAVNTPDLSRLYIISGRMMFDDEEEVMLVEADSCGEA
                     EAAFQQHIDEGIAHLDQVLVISSASLQSLLPDRLIASSLTHTV"
     gene            complement(387..719)
                     /locus_tag="RHZ76_002302"
     CDS             complement(387..719)
                     /locus_tag="RHZ76_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812935.1"
                     /translation="MESEQLITKITQTLKRPDGSEVRIVVEQAFGSGLTPSLGVYVLR
                     RPTTADNWQLCKTAPHKDWRTMSVDEYQKHGRSEMLRYVSIGEILRLSAAIGKPMSYV
                     DTCPGLQG"
     gene            complement(1187..2218)
                     /locus_tag="RHZ76_002303"
     CDS             complement(1187..2218)
                     /locus_tag="RHZ76_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element ISEc33 family transposase"
                     /protein_id="HDV2812936.1"
                     /translation="MPIIVPIPRGERRLMQKAIHKTRDKNHARRLTAMLMLHRGERVS
                     DVARTLCCARSSVGRWINWFTHSGIEGLKSLPAGRSRRWPFEHICTLLRELIKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLHAGTVRRWLPSAGLVWRRAAPTLRIRDPHKDEK
                     MAVIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSYVGGNSKSSALFIALLKHLKATYRRAKTITLIVDNYIIHKSRETQRWLK
                     ANPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETASPFP
                     GGKHGQAKV"
     gene            complement(2374..3555)
                     /locus_tag="RHZ76_002304"
     CDS             complement(2374..3555)
                     /locus_tag="RHZ76_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HDV2812937.1"
                     /translation="MKLFGISSRDSGMDAMFYIQASTKQQASFIFDFLATRLYAHCQL
                     IEPLNETIIPSLSRTTLSLQPWSQNSQTRTSEMQMVTHGLTLSGAVMVEHSRQIVDFC
                     DNLGLRLDAPTGKPGWTELINADCEQLNSPRLYPVLIQPGTEGQFQIYVPAPAVIDAD
                     TVIASVIDECFSDVTSYCLAGTPFPMTEKPVIRMEAHLSDAHTRVFELTFISYNLALS
                     ATVYDKNLRQVLMLCEDFGVTLQGRAGASCHAYMRNEYRRLAHVGVYSRSGAENDVRA
                     YSSDKEETLIRQLMTQATAGSALIPAWHGKQFNGVLMNANPSSATTGFIYLSPEFPQG
                     QFMMTAEVVKSSCTPRGISYIETVDGYRFIVTAYDTKAMHSLTEFLAVNEDYFTPYRW
                     E"
     gene            complement(3564..3860)
                     /locus_tag="RHZ76_002305"
     CDS             complement(3564..3860)
                     /locus_tag="RHZ76_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV2812938.1"
                     /translation="MIHSFKDRRLEKFFRNGKSTAGIPAELINAISCRLETLNNVKNE
                     RELLSGSLRYERLRMTANRYSSIRVNSKYRLFFEWNDGVHNVHLSAHDYKSLIH"
     gene            complement(3924..4391)
                     /locus_tag="RHZ76_002306"
     CDS             complement(3924..4391)
                     /locus_tag="RHZ76_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812939.1"
                     /translation="MDKYFDRSGMAIDNAKIKCIDSVKGTGEYIYRVTCNKCNGRGER
                     NHFYKSRCIACNATGYSLVTTRTCYTLTALYRIYPEAARKISAAQAAERQRAVQSKTS
                     AFNLWCQNHQELVDAITQQDGENSFLNSLKSTLSRKFPLSDKQLTVAARILGM"
     gene            5251..6027
                     /locus_tag="RHZ76_002307"
     CDS             5251..6027
                     /locus_tag="RHZ76_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812940.1"
                     /translation="MPKYLLPAELAEIVCGLLVQPALLNELKTPATHKAFMQDIGELV
                     AKHCGGQIDGVIMPEVRDEDSQADYLTSPDETPYIVVSPGQDMHSLTDNVWRHHARDG
                     WLDHIKGSVEESPDELPTHKECAVRRYTLQRLLLQEETSHANLPVQVVRMCEYSTDDV
                     EQTDSSAEFNVTMALGGHAMLAVKDTDSRLVMALTLLIENGVPSVHVNAGDTDPSVLH
                     IRAAHGGLVLSTDSLNHDFSISPSDQYTYGRPGLLLKAAY"
     gene            6177..6953
                     /locus_tag="RHZ76_002308"
     CDS             6177..6953
                     /locus_tag="RHZ76_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812941.1"
                     /translation="MAKLISTRELATIITALLVKPELLGELDSPEKHRALMEDLGRVV
                     AEHCGGNVTEIALPETDGTAAEGALQNGQPVRYLETKESSPYLIVHPDASLPSVTQNV
                     WMHADHDGWEEHFNGETDALTPEMSEQFRQQVYALLTPESHTTSSEMRLTLQDWQLGE
                     AEIPEEDCQPYQVKVLAENKNVQCEVTNETGTTCFGLILEIDRGVPTLHIDTGSDSLL
                     HIHAAHDGLVLTPDAPSHGFEDAPVDRFSYNSPSLLVPGV"
     gene            7142..7483
                     /locus_tag="RHZ76_002309"
     CDS             7142..7483
                     /locus_tag="RHZ76_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812942.1"
                     /translation="MRELLGMAGAEHQASVMYQTFGHLDAKLGEKHKGHFVFINGQHG
                     DLCVVHSEFSSFDEGPGYFSDRADFIWELVKNDGPCSKVGIYRFDGEYALPKRRNGRR
                     FSGSVTCLQAF"
     gene            7584..7847
                     /locus_tag="RHZ76_002310"
     CDS             7584..7847
                     /locus_tag="RHZ76_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812943.1"
                     /translation="MTNANANDGKTSPVLPTLEQVYALLKANYKPARFADRDGEIWGH
                     EYSWNLARSSLQDLEKYGKSYVSKHSDRMGDGFSFGPDLVIIR"
     gene            8075..8779
                     /locus_tag="RHZ76_002311"
     CDS             8075..8779
                     /locus_tag="RHZ76_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812944.1"
                     /translation="MTMKNTVIPTVTENEMGEVITRHSAYGLVSVSRTSTTGQRLYAS
                     DLSHKEVVTMTFSESEQIERDGVIRHRLAEGRRRSPLLQVSLSPAQWATMITSFGMSD
                     GVPCTINSLIRGDYERQPEIGYIESTRERYERQIRQAAEREMAKLHKKLEVLRLLAVK
                     GKAGKRELDEAYQSLLSVINNLPVNLAFTNQLIQESMVNIVSHGKAELEATAMGVAAR
                     LGMKEMSSLASLEEKK"
     gene            complement(8812..9231)
                     /locus_tag="RHZ76_002312"
     CDS             complement(8812..9231)
                     /locus_tag="RHZ76_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812945.1"
                     /translation="MSELAEKPLITRVAPLERIYYCASRAEGLKPDRHEIRMQSQFLL
                     LGVIWLPPALAMAELERLLKGYDADHSRLLENVLETAHQQFLKFCEPQKREGGGVESH
                     VECPRQDDALQCLRFMSTRYALAVDLCAAYVCSRYGR"
     gene            complement(9319..11703)
                     /locus_tag="RHZ76_002313"
     CDS             complement(9319..11703)
                     /locus_tag="RHZ76_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4165 domain-containing protein"
                     /protein_id="HDV2812946.1"
                     /translation="MKFCRRPLIVAVYTALLFPLYSQAQLYNYSYTDTHGTKVNAEPT
                     REYLNPGSAITLNLISGLDRYERVTVTRSSDNVQMFQSVTGLVTVADRITAADGSEYY
                     GKSMTLPVLGEGTFSLKSETLDNKSNVVATTSYSFSIDITPPVLPDPMQWLRAGFQYG
                     SLDIFGDRTATQAISLSNVSDARSGLDKAEWFAIDSSGVRRTVNAQLNALTGVVQALA
                     AVAAGSTVAPVSPAYYTVGFRVYDKAGNWTETSHRSGIDRTIPSYIQHQVRNAKTGVW
                     EDYVPGMTIFENPVKFRYKRLKSDSTNFNGTDFGWVDQITNSDNTYFYTEATVSYPET
                     YMYYELHTKAGLNVNLQYKTLKFTPGNGVAEAPRYLSGQYHFRDANTWTSSDIVRFTG
                     PDCVDLGRVNVEPRSYVQTATIVGIGSCNVSVGESSCQVSMNYCKTSGKGYVPYQIHI
                     RGTGSYANLGGLQSHFYTYWDMNAPVINSIVSSPLDKTVVMLVTDSDVTNDWTNYMWA
                     TNIFNLTLKDTSNKVSTLSPKNVTVLNYQLKQVTFDYSALPSGKYTATGAATDTYNNT
                     AELTLSNTILHDVTPPVVDISFEGKQADGALVKGLENLVITLADDMTKASLKSITLSG
                     GPTSDKVSVGWYSQGTDKYGLNYPRIFPATDDADSYQLTAVAVDEAGNTTTKVSRFRY
                     VPNNLIEFNTLKTLAVGMGLKTSDNQPLAYLRTNSIRKKDGSLITGAQTGTLTVRKDA
                     AFAVSMNGVAVVPGDSKDITIDFGQGDGVLIPIFPATSGNVGESSFMIELPQIQ"
     gene            11954..12556
                     /locus_tag="RHZ76_002314"
     CDS             11954..12556
                     /locus_tag="RHZ76_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4165 domain-containing protein"
                     /protein_id="HDV2812947.1"
                     /translation="MKNQAIALAMAMLGVSVPASAQIYESIFTDTNGIEVHAPSTRLL
                     LNPASPVTLTLIAGLDRYVNVKITSATGTQLLNTTTTRTSVSDRLKAGDGSEFYGKKV
                     TLPVLGEGRSVVQVSILDLNQSVVATYSYNWLVDTTPLSGNALTGNAAYGSTAGSVWK
                     MGLEATGQFDFTSTNVTDVNGIEKGMFYVYRSNGSLFSTT"
     gene            12568..12804
                     /locus_tag="RHZ76_002315"
     CDS             12568..12804
                     /locus_tag="RHZ76_002315"
                     /inference="COORDINATES: protein motif:HMM:NF023666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HDV2812948.1"
                     /translation="MYHTYSKNSVKGAGIPDSNLDEDFTAKVVIYDNAGNTRTLPLQL
                     FRYDNYLGGPRSAKLRQRGARGFRISGLFSWDDR"
     gene            complement(12958..14186)
                     /locus_tag="RHZ76_002316"
     CDS             complement(join(12958..13871,13871..14186))
                     /locus_tag="RHZ76_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDV2812949.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWKDGRRSRHSDDTDV
                     LLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKTAVP
                     PSKRAHTGKVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFD
                     SETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRS
                     PESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQQASNGLSDNRCLEI"
     gene            14296..14892
                     /locus_tag="RHZ76_002317"
     CDS             14296..14892
                     /locus_tag="RHZ76_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812950.1"
                     /translation="MVYGLENIRIALADNLTTPRITRLQITGGPTADNVELTWSPAGT
                     NVYAPEYPRLFPSFEPSENYALSVTVSDSQSNSKTYTQKFSYLPNNLVQLHNLRTLSV
                     SSALKTSLNEPLAYLSTNVLRKSNGEIAKDVQSATLTVRKDAAFGIKFNGVQAALGES
                     VEVKIDMGLGDNLLVPIYPAENGKVGTSEFMIQIDELK"
     gene            15284..16165
                     /locus_tag="RHZ76_002318"
     CDS             15284..16165
                     /locus_tag="RHZ76_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDV2812951.1"
                     /translation="MSSNNNKLSLVIEESLKDSGEVIQLFPTLKQTIQPKVLLRLGVF
                     VPTLKSTDKGGRKSHHIDATEPLSRLSIVEGEGYSQIQIYGPRLDMDTDFKSWCGIVY
                     ALSNRPLDSEGQLELNFPEFAKFCGFDTKRIDKQLKERFDASLTRIARTNISFIKKIP
                     GTDDQITINMHLVESTYYDSSTGKIIIKPNSKLNTLYRVDGKTRLYLKTLQKLARKES
                     AQALYLYLAELPSTFYRIGFDRLRGRLQLSSHLGNQNATVKKALGQLKEIGFLEYSID
                     KENGDYVLNILKRNMKP"
     gene            complement(16751..17266)
                     /locus_tag="RHZ76_002319"
     CDS             complement(16751..17266)
                     /locus_tag="RHZ76_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2812952.1"
                     /translation="MKISTGMMKSNAPLIILICLMIAFSAYVKSWSLLYTLLAASVSL
                     TVMISLPFIVSPLQHSKFIALRILRLLVITLWTIGLVGVFFSVVERLVWVNADSYPAW
                     LAKELDSPAITDGENFIKATESFEKICGKNKGYLSVVTKHNGIFMRCDDSLSFDSWWK
                     GVYRLKTPETR"
     gene            complement(17917..20373)
                     /locus_tag="RHZ76_002320"
     CDS             complement(17917..20373)
                     /locus_tag="RHZ76_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4165 domain-containing protein"
                     /protein_id="HDV2812953.1"
                     /translation="MRKILTGCIIATAIASVYPAAAQIFSYSYTDTNQLAKTLKPATQ
                     SYLNPASTMTLNLISGLDRYERVSVTRDSDKKVMYSAVTGKISVADRIVAADGTEYYG
                     KDLALPALGEGAFTVINDTLDLQQAVVSSSTYHFTIDTTAPKYTSIYPSQNAGYEMVL
                     SGPVWGLGRGGSGQFSIFADGVDDANGIDKIRLVIKRGNGTVVSDNTLSYDTAAKRAF
                     YPWIKDAVTQPGMPTSDLNEEFTFNFIITDKAGNTLNIPPQRFLFDDQIGEYTPFAVH
                     DSRVSTSVVPGISSGYVPFVRGLSVLENPYRMVIRIPRTNWKPYRNGGITILNNYGGA
                     KVISEDTTYVYVEVKLPQGALDINYYRPVNTYQWSGGDLTQYASWLNWDPASVKSPAW
                     GSPAIERQMSDGTWFNSVNWKYFKASDMPVNLTQIRFNVQARPYDQKITGGATCNIPA
                     GSTSCTVALTQAITNGTTGYLHSGYEVRSTTESTFFMPIWENVVWHTLGPSVTGYDYN
                     DTTNILQVYINQPGDGSYFDTVKLSRVWLSDKSRNNADINVTGTLTGRNTATGNYTYE
                     FNLKLIPEGSYNVQLNAQDSFNNTGSLDYKTVTVDNTPPSISISYETKPITSSVTVYG
                     LENIRIQLADTLTKPSLTRMTLRGGPVSDAVELSWVNLGNNLYAPNYPKIFPSLNDGE
                     TYTLTVQAKDEMNNVKESAVEFSYLPNNLVRLENLKTISVSTALKTSDDTPLAVLYAS
                     QLRKKDGSIATGKQDAILTVRKDAAFGVTVNGVSAAPGESKNVQLDLGLGDSRSFPIF
                     PSTSGVVGTSEFMLNIEELK"
BASE COUNT         5152 a         5145 c         5222 g         5221 t
ORIGIN      
        1 aaataaatat acgggaaaac aaaaaaccgc cacaagggcg gaatttatta gacgggaaag
       61 atttggttat acggtatgtg tcagggagga agcaatgagc ctgtctggta acagtgattg
      121 tagggacgca gacgaaataa caagcacctg atccaagtgc gctatacctt cgtcgatatg
      181 ttgctggaat gccgcttcag cttcaccgca ggagtccgct tcgaccagca tcacctcttc
      241 ctcatcatca aacatcatgc ggcctgagat aatatacagt cggcttaagt ccggggtatt
      301 tacagcgatg ccctgttcgt taatcatcat ttttctccgc cttcaatttt taaatttgtc
      361 agttctgcta cgtgtaaaaa acagcatcag ccctgtaaac ccggacaggt atcaacgtag
      421 ctcattggtt tgccgatcgc ggcgctcagg cgaaggattt cgccgatgga gacgtaccga
      481 agcatttccg aacgcccgtg tttctggtat tcatccaccg acatagtgcg ccagtccttg
      541 tgcggtgcgg ttttgcacaa ctgccagttg tcggcagtgg tcgggcgacg caggacatat
      601 acacccagcg aaggcgtgag gccactgccg aatgcctgct ccaccacaat acgtacttca
      661 gagccgtcgg ggcgtttcag tgtctgagta attttagtaa tgagctgttc ggattccata
      721 ctattaacct cgaccgtcag ttgggttgct gtagggatat tttaccaaag tcggctctct
      781 ggctaataag taagaagtga tttttttcat tcattgaagt ttctttcata tggaatgaat
      841 attaattatt taaactgact cggcctattt taattattat taatcagggt atttaatgtg
      901 caaattggca gggcgctgaa ttaccgggca tccattgccc ggacagttta ttaaaaaaaa
      961 ctgataatgc cccgccggag aattacgcca attcctgtga aggcatggtc tggctgaaaa
     1021 gttacttttc ccggttgccc tgccttgatc ccgtatcgtt tataccggtc tgctcttaca
     1081 tcctacctgg gcagcagctg gctacggtga tcgggtatta aaatagaacc ggtctgtttt
     1141 accgcagatt caacgccccg cacctaaata gctgcgccta ataccgctac acttttgcct
     1201 gaccatgttt tcctccgggg aatgggctgg cggtttccat aaaatggcgg acctttttca
     1261 gtaactgcca cattgagcgg cactgatgat tacgggttat cgtgtcatga agtgcctgcc
     1321 atagccgttc cacatgattc acccacggcg agtaaaccgg ctggtaaatt accctgaact
     1381 tgggatttgc tttcaaccag cgctgtgttt cgcggctttt atggataatg tagttatcaa
     1441 cgatcagcgt gattgttttc gcccgccggt aagtggcttt caggtgcttc agcagagcga
     1501 taaacagcgc tgaacttttg ctgttgccgc ccacgtagct gactttaccc gtgccactgt
     1561 gcagtgcgcc ggccagatag tatttttcgt tctgccccgg cgtcactacc cgtttctgct
     1621 gtccgcgcaa ctgccagtcc gcaccgattt taggattaag gtggatatcc acttcatctt
     1681 cataaaatac cggatgctct gcgctgcatt catccagcgc tttgtggatt accgccatct
     1741 tttcatcttt atgtgggtca cggatacgca gagttggcgc ggccctgcgc catacaagcc
     1801 ccgcagatgg caaccagcgg cgaacggttc ctgcatgtaa ctggcaaccg gttatctcat
     1861 tgatttttat tgccagtaat tcggtgctcc agcgtgaacg ttgataacca aaatcgccgg
     1921 gagaatgctt tatcagctca cgtaacaggg tgcagatatg ttcaaaaggc cagcgtcggg
     1981 agcgccctgc gggtaaggat ttcaggcctt caatacctga gtgcgtaaac cagttaatcc
     2041 agcgaccaac ggatgaacgg gcacaacaga gagttctggc aacatcgctg acccgttcac
     2101 cccgatgaag catcagcatg gccgtgagtc tgcgggcatg atttttatca cgcgttttat
     2161 gaatagcttt ctgcatcagg cgtcgttcgc cacggggtat tggtactatg atcggcatcg
     2221 ctcagtccgg ttggtgattt tggttggttt ggcgattgat cagatcgcac aatccgggct
     2281 gagttccctt tcagtgatct actattccgc gcagctattt agcgtaaaat cgcctttccc
     2341 ggatgccctc cttagtcgtc cggcacgatg gcgctactcc cagcgataag gggtaaaata
     2401 gtcctcgtta acagcaagaa attcggtcag ggaatgcatc gccttcgtgt catatgccgt
     2461 cacaataaaa cggtatccgt ccaccgtttc gatgtagctg atcccccgtg gcgtgcaact
     2521 ggatttcaca acttccgccg tcatcataaa ctgcccttgc gggaattccg gtgacaggta
     2581 gataaagcca gtggtggctg atgacgggtt ggcattcatc agcacgccgt taaattgctt
     2641 tccgtgccac gccggaatca acgcgctccc cgctgtcgcc tgagtcatca actggcggat
     2701 cagggtttcc tctttatctg agctgtaggc gcgaacatca ttttcagccc cggagcgtga
     2761 gtagacgccg acatgagcaa gacgtcggta ctcgttacgc atataggcat gacaggatgc
     2821 accggctcgc ccctgaagag tgacgccaaa gtcttcacat aacatcagta cctggcgcag
     2881 gtttttgtca tagaccgtgg cagagagcgc caggttatag ctgatgaatg tcagctcaaa
     2941 aacccgggta tgtgcgtcac taaggtgtgc ctccatgcgg atcactggtt tctccgtcat
     3001 tggaaacggc gtaccggcca agcagtacga ggtaacatcg ctaaaacact cgtcgatcac
     3061 actggcaatg accgtatcgg cgtcaatgac agccggagct ggaacataaa tctgaaactg
     3121 tccttcagtg cccggttgga tcaggacggg gtatagacgc ggggaattga gttgttcgca
     3181 gtcggcattg atcagttctg tccaaccggg ttttccggtg ggagcatcaa ggcgaagacc
     3241 caggttatcg cagaagtcga cgatctggcg ggagtgctcc accatcactg ccccggagag
     3301 tgtcagaccg tgcgtaacca tctgcatctc actcgtccgg gtctgggagt tctgggacca
     3361 gggctgtaat gaaagcgtcg tccgacttaa ggagggaatg atggtttcat tcagaggttc
     3421 aataagctgg caatgcgcat acagccgggt ggcgaggaag tcaaatatga agcttgcctg
     3481 ttgttttgtg ctggcctgaa tataaaacat cgcatccatg cctgaatccc gggatgaaat
     3541 accgaaaagt ttcatttttt accttaatgg atgagcgatt tataatcatg cgccgacagg
     3601 tggacgttat gtacgccatc gttccactca aagaatagcc gatattttga attaactcga
     3661 atggaagaat accggtttgc ggtcattctt aagcgttcgt accgcaaact accagataac
     3721 aattcgcgtt cgtttttaac attatttaac gtttccaatc tgcatgaaat cgcattaatc
     3781 agttcagcag gaatgcctgc cgtggatttt ccattacgga aaaacttttc caatcgcctg
     3841 tctttaaatg aatggatcat attttcctcc gtaacgaaat gagtttaaat aaataagaat
     3901 taaaaaagcc tgaatctaat ttattacatc ccgaggattc tggctgcgac cgtcagttgt
     3961 ttatcactta atggaaattt gcgtgataac gtcgacttca ggctgttcaa aaaactgttc
     4021 tcgccgtctt gctgcgtgat ggcgtccacc agttcttggt ggttctgaca ccagaggttg
     4081 aacgcggaag tctttgattg aacagcacgt tgacgctctg ctgcctgagc ggctgagatc
     4141 ttgcgagcag cttctgggta tatgcgatac agtgcagtca gggtgtaaca ggtacgggta
     4201 gtgaccaggc tatagccagt ggcattacag gcaatacagc ggctcttata aaagtgattg
     4261 cgttcgccac gtccattgca tttattgcaa gtaacacggt aaatatattc cccggtgcct
     4321 tttactgaat caatacactt aatcttagcg ttatcgattg ccattccgct acggtcaaag
     4381 tatttatcca tttttgattc ctcaggtttg gtctgaagta ataatgacag attggcctcg
     4441 ctggctaata acctgaacga acgttttttg aaaataaatc aggaaggatg attgagatga
     4501 tttatatatg caggattcaa ttttaatgga gggtattttc agaattatct gtttacaaaa
     4561 aacgaaacaa atcctaatga ggcatttatt gcatcacaat aaaagtgatt gcttttcact
     4621 catttgagta gcgatgctta agtatttatc cgttttaagg tataacagca gcgtgatacg
     4681 gctcagggaa tgcctgtccg tcaccttaat gatgctttac ttccggttgc cgaaacagtc
     4741 catatggctg acatgtataa cacgcacggt ctaacgcagt tccggggggt ggcacagcgg
     4801 cgtgatgccg ctttaatctg gaaaacgcag gcatctactg gtgcctcagg atcgcggcac
     4861 tcgcaagctg aaacaccaga gcctgttcgt cttccggctg tatcgctcgt ccccgtctac
     4921 aaaacccgtc tcatccgcag gtatcgctgg cgcactccca gacggttaga ggctttcggg
     4981 aactgcctgg gtcaaagaac accggcgttc aggtagctgt tgaaatggac atctacacgt
     5041 ttttgttcaa gtgattcagt tgtggtgagg gcataacggg taatgccctc ccgtgaagcc
     5101 ctgagaaaag ttacttttcc aggattaatg cagtcttgct tttaatataa attcagggga
     5161 tattttttta aaaaaatacc tgtcgtcaac ttattagaaa agtaagtcaa aatgctagag
     5221 tgttctcatt caaaaagagg agaactaacc atgccaaaat atttgttacc tgccgagctt
     5281 gcggaaattg tttgtggatt actggtgcaa ccagcattat tgaatgaatt aaaaacacca
     5341 gccacgcata aagcatttat gcaggacatc ggcgagctgg tggcgaaaca ttgcggcggt
     5401 caaattgacg gcgtgatcat gcctgaggta cgcgatgaag acagccaggc cgattacctg
     5461 acatctccag atgaaacccc ttatattgtt gtctctcctg gccaggacat gcactccctc
     5521 acagataacg tctggcgtca tcatgcccgg gatggctggc ttgaccatat caaaggcagt
     5581 gtcgaagaaa gccctgatga actgccgacc cacaaagagt gcgctgtacg ccgctacacc
     5641 ctccagcgtc tgctccttca ggaagaaacc agccatgcta atctgccagt acaggtggta
     5701 cggatgtgtg agtacagcac cgacgatgtt gagcagaccg attcttcagc agagtttaac
     5761 gtcacaatgg cgctcggtgg tcatgccatg ctggcagtaa aagatacaga ttcccgtctg
     5821 gtgatggccc tgacactctt gattgaaaat ggagttccct ctgtccacgt taatgcgggt
     5881 gacactgatc cttcagttct gcacatccgg gcagctcacg gtggtctggt tctttcaacc
     5941 gactccctta atcatgattt cagtatttcg ccctctgacc agtataccta cggacgccct
     6001 ggattattat tgaaagccgc ctactgattt attaattcaa aatccgtcat tattatttac
     6061 ccccattttt cgcccctaac ttatggggcg agtatatttt aaaaaacttt ctttcaggtt
     6121 attagccagc aaactagaac tggtagaatt aattcatcag agaaaagggg actatcatgg
     6181 ctaaattaat ttcgacgcgt gaactggcaa caattattac tgcattgctg gtaaaaccag
     6241 aattactggg cgagctggat tccccagaaa aacatcgcgc cctgatggaa gatctggggc
     6301 gtgttgtggc agagcattgt ggcggtaatg tcactgaaat cgcactaccg gagaccgatg
     6361 gtacggctgc tgaaggcgcg ttgcagaatg gccagcccgt ccgctatctg gaaaccaaag
     6421 aaagctctcc gtatctcatc gtacaccctg acgcctcact gccgtcggtg acgcaaaacg
     6481 tctggatgca cgctgatcat gatggctggg aagagcattt caacggagag accgatgccc
     6541 tgactccaga gatgagcgag cagttccgcc agcaggttta tgcactgctg acgccggaat
     6601 cccataccac ctcttcagaa atgagactga ctttgcagga ctggcaactg ggtgaagccg
     6661 agatcccaga agaagactgt cagccctacc aggtgaaggt gttagcagaa aataaaaacg
     6721 ttcagtgcga agtgacgaat gaaaccggca ccacgtgttt cggcctcatt ttggagatcg
     6781 accgtggtgt accgacgctg cacattgata cgggcagtga ttcactgctg cacattcacg
     6841 cggcacatga cggcctggtt ctgacaccgg atgcaccgag ccacggattt gaagatgctc
     6901 ctgtagatcg cttttcttat aacagcccgt ccctgttggt gcctggggtg taaccaggca
     6961 aactgcccag cggtattttg gccccgggta aaattattca gaacaggagt caggcagatg
     7021 ggcgatgttg ccacactacc accgaaaatt aacgatggcg tacttcgacg ctggaatgct
     7081 ctcggtaaag cggataaaga ggcgctggcc gactatggcg ttagcgtgaa ggaggagtga
     7141 attgcgcgaa ttacttggaa tggcaggtgc agaacatcaa gcctccgtaa tgtatcaaac
     7201 gttcggacac ctcgacgcga agctaggcga aaagcataaa ggtcacttcg tttttatcaa
     7261 cggccagcac ggcgatctgt gcgttgtgca tagtgaattt tcatcctttg acgaaggtcc
     7321 aggctatttc agtgaccgtg ccgacttcat ctgggaatta gttaaaaacg acggcccatg
     7381 ctctaaggtc gggatttacc gatttgacgg cgaatacgcg cttcctaagc gccggaatgg
     7441 gagaagattt tcaggaagcg taacgtgcct gcaagcattc taattccgtg ctgcatgagc
     7501 gccgacaggt cttccgtaca acgggaggcg gcacagttcg cgtgcaacca tccccacaca
     7561 ataactcgac tgagtacata ataatgacga acgctaacgc aaatgacggg aagaccagcc
     7621 cggtattacc cactctcgaa caggtctatg ctctgctcaa ggccaactat aaacccgcac
     7681 gatttgctga tcgggatggg gaaatctggg ggcatgagta ttcatggaac ttggcaagat
     7741 ccagtttgca agacctggag aaatacggga aatcatatgt atccaagcat tccgaccgga
     7801 tgggcgatgg attcagtttt ggtcctgacc tggtcattat tcgctgacat acgcacggaa
     7861 gtgagtaaga tggctggctg cgccggagaa ctagagtaat gaagggtaaa aatatcgtgg
     7921 ctcgtgtcgt gcgggaagcg gcctataagt gggaaactcg ccaccaattg cagcaggcgt
     7981 acaattcagc gagtgacgtg ggaaaagttc ccagacattc cggttccgca tctgatttac
     8041 gccagacgct gtcattttta attctttgta gggcatgacg atgaaaaaca cagtaatacc
     8101 gacggtgact gaaaacgaga tgggagaggt gatcacccgc cactcggctt acggactggt
     8161 atcagtaagc cgcacgtcca cgacgggaca gcgcctttat gcctccgacc tcagtcataa
     8221 agaggtagtc acgatgacct tcagtgaatc agagcagatt gaacgcgacg gtgtaatccg
     8281 tcatcgactg gcggaaggcc gacgccgctc accgctcctg caagtcagtc tgtctccggc
     8341 gcagtgggcc acgatgatca catcgttcgg aatgagtgac ggcgtgccgt gtaccattaa
     8401 cagcctgatt cgcggcgatt atgagcggca gccggagatt ggctatatcg agtcgacacg
     8461 cgaacgatac gaacggcaga tccggcaagc cgctgagcgt gaaatggcga aattgcataa
     8521 aaagctggaa gtgctgcgtt tgctggcggt aaaaggcaag gctggaaagc gtgagcttga
     8581 cgaagcctat cagtcgctgt taagcgtaat caataacttg ccggtcaatc tggccttcac
     8641 caaccaactc attcaggaat ccatggtgaa tatcgtctca cacggcaagg ccgaactcga
     8701 agccaccgcc atgggcgtag ccgctcgtct gggtatgaaa gaaatgagca gccttgcctc
     8761 gctggaagag aagaagtaac cggtgcgccc gcccgtaaat catgaaatgc actaacgtcc
     8821 atacctgctg cacacatacg cggcacacag atcaacggcc agcgcatacc gcgttgacat
     8881 aaaccgtaaa cactgaagtg cgtcgtcctg tcggggacat tctacgtggc tttctacgcc
     8941 ccccccttct ctcttctgcg gctcacagaa cttcaggaat tgctggtggg cagtctccag
     9001 gacgttttcc agtaaccggc tgtggtcagc gtcatatccc ttcagcaggc gttccagttc
     9061 tgccatagcg agtgcaggtg gtaaccagat gacgccaagc agcaaaaact ggctctgcat
     9121 tcgaatttca tggcgatctg gcttcagacc ctctgcccgg gaagcgcaat aataaatcct
     9181 ctccagggga gctacacgcg tgataagagg tttctcagca agttctgaca tgcgactaaa
     9241 tcagcctgtt tcgtttcttt ctgattacgt taagacacta tgaaaaagcc ccgtaatacg
     9301 gggccggttc ggaggcagtt actggatttg cggcagctcg atcatgaagc ttgattcacc
     9361 gacattgccg ctggtggccg ggaagatggg gatcaggacg ccatcaccct gcccgaaatc
     9421 tatcgtgatg tctttcgaat ccccgggtac gacggccacc ccattcatac taaccgcaaa
     9481 ggccgcatct ttgcggaccg tcagcgtgcc ggtttgtgct ccagtgatca gcgaaccatc
     9541 ttttttccgg atgctattgg tacgcagata cgccagcggc tggttatctg atgtcttcag
     9601 ccccataccc acggccagcg ttttgagagt gttaaattca atcaggttgt tcggtacata
     9661 acggaaccgg ctgacctttg tcgtggtatt accggcttca tctaccgcaa ccgcagtaag
     9721 ctggtaggaa tccgcatcat ctgtcgcggg gaagatacgc gggtagttca gcccgtattt
     9781 atcagttccc tggctgtacc agcccacact gactttgtca gaagtgggtc cgccgctcag
     9841 cgtgatgctt ttcagggaag cttttgtcat atcgtcggcc agtgtgatca ccagattttc
     9901 cagccctttg accagtgctc cgtcagcctg ctttccctca aaactgatat cgacaacagg
     9961 cggcgtgacg tcatgcagga tggtattcga tagcgtcagt tctgccgtgt tgttgtaggt
    10021 gtccgtcgcg gcacccgttg cagtgtattt cccggagggt aacgcgctgt aatcaaacgt
    10081 cacttgtttc agctggtagt tcaggaccgt aacgtttttc ggcgacaagg tagagacttt
    10141 attactggtg tccttcagtg tgaggttaaa gatattggtg gcccacatat aattggtcca
    10201 gtcgttagtt acgtcactgt ccgtcaccag cattaccacc gttttatcca gtgggctgga
    10261 gacgatactg ttgataaccg gggcgttcat atcccagtag gtgtagaaat gcgactgtag
    10321 tccgccgaga tttgcgtatg aaccggtccc tctgatatga atttggtacg gaacatagcc
    10381 tttcccgctc gtcttacagt aattcatgct gacctggcaa ctgctctcac ccactgaaac
    10441 attgcaactg ccaatgccga cgatagtagc agtttgcaca taagagcgcg gttcaacgtt
    10501 tacacgaccc aaatctacgc aatccggccc ggtaaacctg acgatgtcac tcgaagtcca
    10561 tgtattagca tctctgaaat ggtactgccc tgacaaatag cggggggctt cagccacgcc
    10621 gttacccgga gtaaatttca gcgttttgta ctggaggttg acgttaaggc ccgctttagt
    10681 atgaagctcg tagtacatat aggtttccgg gtagctcacc gtggcttcag tataaaaata
    10741 ggtattgtcg gagttggtaa tctgatcgac ccagccgaag tctgtcccgt taaaattggt
    10801 tgagtccgat ttaaggcgtt tgtacctgaa tttgactggg ttctcgaaaa tagtcatgcc
    10861 aggaacataa tcttcccaga cgcctgtttt cgcattccgc acctgatgtt gaatgtagct
    10921 gggtattgta cgatcaatac cagagcgatg tgatgtttct gtccagttcc cggctttgtc
    10981 ataaacccga aaacctacgg tgtaatatgc cggactgacg ggcgcaaccg tgcttcctgc
    11041 cgccaccgcc gccagtgcct ggaccacccc cgtgagtgca ttcagctgag catttacagt
    11101 ccggcggaca ccgctgctgt caatagcaaa ccactccgct ttatccagtc ctgacctcgc
    11161 atcagagacg ttcgataacg aaatggcctg ggttgcggtc ctgtcgccga aaatatccag
    11221 acttccgtac tgaaaaccag ccctgagcca ctgcatcgga tctggtagaa cgggaggagt
    11281 gatatcgatg ctgaaagagt agctggttgt cgccacgaca ttcgacttat tgtcgagtgt
    11341 ttcggattta agagaaaaag taccctcgcc cagtaccggc agtgtcatgg acttgccgta
    11401 gtactcacta ccatcagcgg cagtgatcct gtcggccact gtcaccaggc cggtgacact
    11461 ctggaacatc tgcacattat cggatgaacg tgtcacggta acgcgctcgt agcggtcgag
    11521 accagatatc agattcagcg taattgcgct gcccgggttg agatactctc tggtgggttc
    11581 agcattcact ttcgtgccgt gtgtgtcggt atacgaatag ttgtaaagct gagcctggct
    11641 gtaaagcggg aacagcagtg ccgtatagac ggcgacaatc aacggacgtc ggcaaaattt
    11701 catttcggtt ctcctggttg gccagaaaat tttacctgaa tcgttttctt cgcccgggcg
    11761 ctaagagggg taattgttca gtaaaacatc gtccgtttgt gtgctttttg ccagatttaa
    11821 tcggcaggcc tgcgggaaat accccatccc aatgtccgtt ttaggctgat taattcccca
    11881 gaaatccccc ccttaacgga aaaatccgca gggcagacgt ttaaaatagc aacaactaaa
    11941 aggggaatac tgaatgaaaa atcaggctat agctttggct atggctatgc tcggagtctc
    12001 agttccggca tctgctcaaa tttatgaatc catcttcaca gataccaatg gtattgaagt
    12061 tcacgccccc tcaacccggc tgttgctgaa tccggcctca ccggtaaccc tgacgctcat
    12121 tgccggtctt gaccgttatg tgaatgtcaa aatcaccagc gccaccggca cgcaactgct
    12181 gaacaccact actacgcgca ccagcgtatc cgaccgtctg aaggccggag atggcagcga
    12241 attttacgga aaaaaagtaa ctttaccggt gcttggcgaa ggcaggtcgg ttgtgcaggt
    12301 cagtattctg gatctgaatc agtccgttgt agccacctac agctacaact ggctggtaga
    12361 caccacaccg ctgtctggta atgccctcac aggcaacgcc gcctatggat caactgcggg
    12421 tagcgtctgg aaaatggggc tggaggccac ggggcagttt gattttacct caaccaatgt
    12481 cacggacgta aatggcatcg agaaaggtat gttttatgtc tatcgcagta acggcagttt
    12541 gttcagtacg acgtgactgg tcagaagatg tatcacactt actcaaaaaa ctcggtcaaa
    12601 ggcgcgggta ttcccgacag caatctggat gaagatttca cggccaaggt agtcatttat
    12661 gacaacgcag gaaacacccg aacccttccg ttgcagctct ttcgatatga taactatctg
    12721 ggcggtccga gatccgcaaa gctccgccag cgtggtgcca ggggtttccg gatatccggc
    12781 ctattcagct gggatgaccg ttaatgagaa tccgattcat cttgtatatc gcgtgccaaa
    12841 gtccaactac cggtcatatg ccgagggcgg cctactattc tataacctgt attcttcccc
    12901 gaccgagatt gcgacggaca gcacctatac ctacgttgaa atgatctgga tttgccccta
    12961 tatttccaga cacctgttat cacttaaccc attactggcc tgctgccgca gatattcccg
    13021 tggcgagcga taacccagtg cactatgcgg atgccattcg ttataatgct cgaacgcctc
    13081 tgcaaggttc tttgctgccg ttaacccgtc tggtttaggc atgatactga tgtagtcacg
    13141 ctttatcgtt ttcacgaagc tctctgctat gccgttactc tccggactcc gcaccgccgt
    13201 gttcttcggt tcaagcccca acatccgggc aaactgccgt gtttcattag cccggtagca
    13261 tgaaccatta tccgtcagcc actctactgg agacgccgga agctcgttgc cgaagcggcg
    13321 ttccaccgct cccagcatta cgtcctgtac tgtttcactg tcgaagccgc ccgtagtgac
    13381 tgcccagtgc agtgcctcac ggtcacagca gtccagcgcg aacgtgactc gcagtttttc
    13441 tccgttatca cagcggaact cgaacccgtc agagcaccat cgttgattgc tttctttcac
    13501 ggccactttg ccagtatgtg cccgtttcga tggcggtaca gcggtttttc gctcaagcaa
    13561 cagcgcattc tggcgcatga tccggtaaac acgtttggca ttgatcgcag gcataccatc
    13621 aagttcggcc tgtctgcgaa gcagcgccca tacccgacga taaccatacg tgggcagctc
    13681 tccgataaca tggtgtatac ggagaagcac atccgtatca tctgagtgac ggctgcggcg
    13741 accatctttc cagtcatcgg ttcgtctgag aattacgtgc aactgcgcac gcgacacccg
    13801 gagacaacgg ctgactaagc ttactcccca tccccgggca ataagggcgc gtgcgctatc
    13861 cacttttttg cacgcccgta ttcaacggct tctttaagga gttcattttc catcgttttt
    13921 ttgccgagca ggcgctggag ttctttaatc tgcttcatgg cagcagcaag ttcagaggca
    13981 ggaacgacct gctctcctgc ggccacagca gtaagacttc cctcctggta ttgcttgcgc
    14041 cagagaaata actggctggc tgccacaccg tgttgccggg caacaaggga gaccgtcatt
    14101 cccggttcaa aactctgctg aacaatagcg atcttttcct gtgtagtacg ccgtctgcgt
    14161 ttctccggtc ctaagacatc aatcatctgc tctccaatga ctagtctaaa aactagtatt
    14221 aagactatca cttatttaag tgatattggt tgtctggaga ttcagggggc cagtctatga
    14281 tcctgcccta gtacggtggt atacgggctg gagaatatcc gtatagctct ggcggataac
    14341 ctgacaacac cccggatcac ccgtcttcag ataaccggag ggccaacggc agataacgtt
    14401 gagctcacgt ggtcacctgc tggcacgaac gtttatgcgc cagaatatcc aaggctgttc
    14461 ccctcattcg agccttccga aaactatgcg cttagcgtga cggtctccga cagtcagtcg
    14521 aacagcaaaa cttataccca gaaattcagc tacctcccga ataacctagt gcagttgcat
    14581 aacctgcgaa ccttatccgt cagttcagca ctcaaaacgt cgttaaatga gcctcttgct
    14641 tatctgtcaa ccaacgtgtt gcgtaagtcc aacggtgaga ttgcaaaaga cgtccagagt
    14701 gcgacgctaa cggtacgtaa agacgctgcc ttcggtatta agttcaacgg cgtgcaggcc
    14761 gcgcttggtg aatctgtaga ggtgaaaatt gatatggggc tcggtgataa ccttttagta
    14821 cctatatacc ctgctgaaaa cggcaaagtg ggtacatccg agttcatgat ccagatcgat
    14881 gagctgaagt aatgagaatg ggccgtcggt agtactggcg gcttaacgaa agtaactttt
    14941 tctgtaaggg ttgaagtgtg cttctgatgt ttttagaacg gatcggaatt tactcacatt
    15001 ttgatcgtga tgattcaata cgtgacagag catagttatc aacaggatag aataataata
    15061 aagatcaata gaggatcaat agcagatcaa aaaggaataa cgatcctcgc agcccagtga
    15121 tatcgggcct ctcagcggat cagcatccac tgtacagggg gagaaataca ctatgtcggg
    15181 taatccatcc actgtaccgg gcgcataatc cactataccg ggtagattcg atacactata
    15241 gcgggtagga tgtagatctt atgcacaaaa cggatctccc aatatgtcaa gtaataacaa
    15301 caagttatct ctcgtcattg aagagtccct aaaagattca ggggaagtta tacaactctt
    15361 tcccacatta aaacagacca ttcaaccgaa agttttactc cgcctaggtg tgtttgtgcc
    15421 gactttgaaa tctacggaca agggggggag gaagagccat catatcgatg ccactgagcc
    15481 gctatcaagg ttatctattg tagagggtga aggttacagc cagatccaaa tctacgggcc
    15541 gcgtctcgat atggataccg atttcaaatc atggtgtggg atcgtttatg cgttgtctaa
    15601 ccggccgctt gattcagaag gtcagctgga gctgaatttt ccggagtttg caaaattttg
    15661 tgggtttgat accaagcgca ttgataaaca actgaaagag cgctttgatg cttcgctgac
    15721 gcgtatagcc cggaccaaca tttcatttat taagaaaata cccggtacag atgatcagat
    15781 aacgataaac atgcacctgg tggaatcgac gtattacgac agttccacgg gaaaaatcat
    15841 catcaagcca aattcgaagc tcaatacgct gtatcgcgtc gacggtaaaa cccgactcta
    15901 cctgaagaca ttgcaaaaac tggcccgtaa ggagtccgca caggccctgt acctgtatct
    15961 ggctgagctg ccgagcacct tttaccgcat cggattcgat cggcttcgtg gacgactcca
    16021 actgtcatca cacctcggta accagaatgc cacggttaaa aaggcgctgg gccagcttaa
    16081 agagatcggt tttctggaat actccataga caaagaaaac ggcgattacg tactgaatat
    16141 tctaaagaga aacatgaagc catgaccgat gccggataat gcacctgcct gccgcgtggg
    16201 tggctgcgtt acccggtata gtggacattc ttctcctttc cactctgctt tacccggtac
    16261 aatggatctc cactacaccg ggtagtgtca ggcctaaaat tacgatgcag cccctcctct
    16321 tcagccttac actgaatatt tagtgtgttt tttcaccaaa aatgtgatct atacccggta
    16381 cagtggagtc acagaaatgt gatccgtacc cggtatagtg gatgagaact actgctaatc
    16441 ggttaccggg aatgtggtgg tactgggtgg aaaggtttac ccggtatagt ggatcgtggc
    16501 agtgtcatct ttccggatag catgacgtca cggttaggaa accgtacccg gtatagtgga
    16561 tagcgaatgg tgtacaaccc tgttaacgtg atgatgctga agtgaaagtt taacccggtt
    16621 tagtggagcg acttaaagcg cgtaccagta gctgatgggg gagcagcacc cggttcagtg
    16681 gaggggcgga tatcgtcgta aaggtcttaa ctaaaagatg accattcagt cgcagcatta
    16741 aagaaatata tcatcgagtc tcaggggttt taagccggta gacacccttc caccaggagt
    16801 cgaaagacaa gctatcatcg cagcgcataa atatgccgtt atgcttggtg acaacgctaa
    16861 gatagccttt atttttcccg cagatcttct caaaagactc tgttgcttta ataaaatttt
    16921 caccatccgt gatggccgga gaatccagtt ccttcgccag ccatgccgga tagctgtccg
    16981 cattaaccca tacaaggcgt tcaacaactg aaaagaagac gccgacaagg ccaatcgtcc
    17041 aaagcgtaat aactagtaaa cgcaaaattc gtaaggctat aaattttgag tgctgtagag
    17101 gcgaaacgat aaaaggcagt gagatcatga ctgtgagtga gactgatgcc gccagcaagg
    17161 tgtataacaa tgaccatgat ttaacatagg cggaaaaagc gatcattaaa caaatcagaa
    17221 tgatgagcgg tgcattgctt ttcatcatgc ccgtgctaat tttcattctt ttggggctcc
    17281 attttgtacg agttttgaga gactgcgttt gtaatcatcc gctgccttag aaaagttacc
    17341 gtcattctgt accggcgtaa agcgaatgcc aaaaaacgcc tcaatgacgg aggaaagcgt
    17401 cactatcggt cctgacgagg catgggcttt tcgctcccga gcctcacgct ccgcaaagtg
    17461 gatcaccggt cccctagaaa atgagagtga gtcatgggaa gaccagtctg tttatagcca
    17521 cagtagttaa acgcaccccg ccagtccgtc gctgtttgac atcatggccg cgaattaggt
    17581 taacgtcgcc cggtatagtg taatcccagg ctgaacctgt gtttgcagct ggtggccctg
    17641 catccatcac catgacaggc gctggccgca ggaatcgcgt gccggatgaa gggtatccac
    17701 tataccgggt gaatgatatg ttgatccacg ctgacgggta gttttatgag caaacccgca
    17761 gcgagtggca ggcctgtgtc attcgcagtt ttactgatgg gacggaaggt tatccacaaa
    17821 aacggtgaat aaccctgtga ccggaatctg gataatcatc attgcggcga caacaacaga
    17881 aagaagtggc ccggacttgg ccaccctacc ctgcttttac ttcagttcct ctatattcag
    17941 cataaattca gaagttccaa cgacgccgga agtcgaagga aaaataggga agctgcggct
    18001 gtcacccaga cccagatcca actgaacatt tttgctctcc ccaggagctg cggaaacccc
    18061 attcaccgtg acgccgaagg ctgcatcctt acgcaccgtc agtatcgcgt cctgtttccc
    18121 ggttgcgata gacccgtcct tcttacgcag ctggctggca taaagaaccg ccagcggtgt
    18181 gtcatcggac gttttcagcg cggtactaac ggagatggtc ttaaggtttt ccagacgaac
    18241 caggttgttc ggcaagtaac taaattcgac ggcgctttct ttaacgttgt tcatctcgtc
    18301 tttggcctgc acggtaagcg tgtaagtttc gccgtcgtta agtgaaggaa agatcttcgg
    18361 gtaattcggg gcataaagat tatttccgag gtttacccac gacaactcaa cagcatcaga
    18421 taccgggcca ccgcgaagcg tcattcgcgt taaagagggc ttggtcaggg tatctgcgag
    18481 ctgaatacga atgttctcca gtccatacac cgttacactg ctggtgatag gtttggtttc
    18541 gtagctgata ctgatggacg gcggcgtgtt atcgaccgtc acagtcttat agtccagact
    18601 acccgtattg ttgaacgaat cctgggcgtt gagctgcacg ttatagctgc cctccgggat
    18661 tagtttcagg ttgaactcgt atgtataatt cccggttgct gtgttacgcc ccgttagtgt
    18721 tccggtcaca ttgatatccg cgttattgcg ggatttgtcg gacagccaca cccggctcag
    18781 tttaacggta tcgaaatacg atccgtctcc cggctggttg atgtaaacct gaaggatgtt
    18841 ggtcgtatca ttgtagtcat agccagtgac cgaaggcccc agcgtatgcc agacgacgtt
    18901 ttcccagata ggcatgaaga aggtggattc tgttgtggaa cgtacctcat aaccactatg
    18961 cagatagccg gtggtgccgt ttgtaatcgc ctgtgtcagg gcgacggtac acgacgttga
    19021 cccggcaggg atgttgcagg tggcaccgcc agtaattttt tggtcgtagg gccttgcctg
    19081 cacattgaaa cggatctggg tcaggttcac cggcatatct gaagccttga agtatttcca
    19141 attgacgctg ttaaaccagg taccatcgga catctgtcgc tcaatcgccg gactccccca
    19201 tgcaggactt tttaccgaag ccgggtccca gttcagccag gaggcgtatt gcgtcagatc
    19261 gcctccggac cattggtacg tgttcactgg gcgatagtag tttatatcca gagcgccctg
    19321 gggaagcttc acctcgacgt acacataggt cgtgtcttct gaaataacct tagcaccgcc
    19381 ataattgtta agaatggtga tgccgccgtt acggtatggc ttccagttgg ttcgggggat
    19441 acggatgacc atacggtatg ggttttcgag tacagatagc ccccgaacaa acgggacgta
    19501 ccctgaagag atccccggga ccacactcgt actgacgcgt gagtcatgaa ccgcaaaggg
    19561 ggtgtattca cctatctggt catcaaataa aaagcgctgc ggcggaatat tcagcgtgtt
    19621 tccggcttta tctgtgatga taaaattgaa ggtaaattct tcattgagat cgctggtggg
    19681 cataccgggt tgagtgaccg cgtccttgat ccaggggtaa aaggcacgtt ttgccgccgt
    19741 atcgtaactg agtgtgttgt cggacacgac tgtgccattt ccgcgcttga tgaccagacg
    19801 gatcttgtct atgccattcg catcatcaac tccatcagca aagatggaaa actggccaga
    19861 tcctccgcgc ccaagccccc aaactgggcc actcaggacc atttcatagc ctgcgttctg
    19921 gctcgggtag atgctggtat actttggcgc tgtagtgtcg attgtgaaat gatatgttga
    19981 agagctgacg acagcctgct ggagatccag agtatcgttg atgacggtga aggccccttc
    20041 accgagtgcg ggtaacgcca agtcctttcc gtaatattcc gtgccgtctg cggcaacaat
    20101 gcgatctgcg acgcttatct ttcctgtcac tgcggaatac atgacctttt tgtcactgtc
    20161 cctggtcaca ctcactcgtt cgtatcggtc aaggccggag atcagattca gggtcattgt
    20221 gcttgccgga ttaaggtagc tctgcgtggc aggcttaagc gtctttgcca gctgattggt
    20281 gtcagtatac gaatagctga aaatctgagc cgccgccgga taaactgaag ctatggccgt
    20341 ggcaatgata cagcctgtca gaattttacg catgtgatac tcccgggatg tatttgcccc
    20401 tattttacga aatcttttca cctgggcttg ctggttagcc ggggtatttc acggcataaa
    20461 acttttaaat gttgccgtga tagcaatgtt gtcgggtcag aacaaaacga aagttaaggc
    20521 aacttcaaca gggctgatta cggtggaaga aaagtaactt ttctcttgag aagaaatagg
    20581 aaaagcagaa attaggacga ttaaatgagg tgaaatgtca gcaacaggcg atgattgtcc
    20641 atcatcgtaa gggagggtac agggtgccgc tgccttactg gcatcatgtt gatgtccagc
    20701 tcttcatacc cgtttgtacc ttagccggaa ctgttgttca
//
LOCUS       DAPHBJ010000072        20581 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.072, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000072 DAPHBJ010000000
VERSION     DAPHBJ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.072"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(365..1561)
                     /gene="hemY"
                     /locus_tag="RHZ76_002321"
     CDS             complement(365..1561)
                     /gene="hemY"
                     /locus_tag="RHZ76_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HDV2812954.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRHDGLMLTL
                     QNNPPQ"
     gene            complement(1564..2757)
                     /gene="hemX"
                     /locus_tag="RHZ76_002322"
     CDS             complement(1564..2757)
                     /gene="hemX"
                     /locus_tag="RHZ76_002322"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDV2812955.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPQGDTPAA
                     APQGE"
     gene            complement(2779..3519)
                     /gene="hemD"
                     /locus_tag="RHZ76_002323"
     CDS             complement(2779..3519)
                     /gene="hemD"
                     /locus_tag="RHZ76_002323"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDV2812956.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3516..4457)
                     /gene="hemC"
                     /locus_tag="RHZ76_002324"
     CDS             complement(3516..4457)
                     /gene="hemC"
                     /locus_tag="RHZ76_002324"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDV2812957.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDSRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4844..7390
                     /gene="cyaA"
                     /locus_tag="RHZ76_002325"
     CDS             4844..7390
                     /gene="cyaA"
                     /locus_tag="RHZ76_002325"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDV2812958.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7430..7750)
                     /gene="cyaY"
                     /locus_tag="RHZ76_002326"
     CDS             complement(7430..7750)
                     /gene="cyaY"
                     /locus_tag="RHZ76_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HDV2812959.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDYWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7798..9123)
                     /locus_tag="RHZ76_002327"
     CDS             complement(7798..9123)
                     /locus_tag="RHZ76_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HDV2812960.1"
                     /translation="MNGQIITVALILLGHIFLTPAVQAIGFDYYNDHGVMSYGKGYGE
                     NEKIIAQFPKMNRADLRLVTNISGERELVEGYIPTDEISIKNEEYHWVTDGRVILWRG
                     KIVSNPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNS
                     TTLMDSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYL
                     NGVHLTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESR
                     VRWRKQLTPDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIP
                     LDTPDLVIDRSFNSGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDT
                     EVRITPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            complement(9120..10589)
                     /locus_tag="RHZ76_002328"
     CDS             complement(9120..10589)
                     /locus_tag="RHZ76_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HDV2812961.1"
                     /translation="MHYRNVVCLLSCSLFLSSAWGCRLDEPEHNIYQKQGKGVVYLRP
                     YEKTNLSLPQINYKRLRLLPNLLIDPTKLKDWETVPPATDLTTDVVYSGANATLPHYS
                     YYSDGRAILYAGEIVQNPPGTPPVDISSFQAWGDFAADKYSLYYEGKRTDSNQQLNRR
                     TLRQVEFNPQWKPDWLGLILRDKHYLYANGQRLDDPDTFTVLAQKSWDQRGKFSTAFN
                     PCLPAPFGPWDTLARTRTKILINSEQLDADPNTFSVVRWMPGSLLTWRDKNGLQRKVL
                     DKENLAWDEDLTKHCLDFSLLEKKVFWRKGPACKQEELPGLDPEQFHPISDAVAQYQD
                     SLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAEGWPYHSGLHVFES
                     DGPLILLDNHSPDEREAHLNDHPFLRRWYARDNRYVYSFDGAQLWRYRTADPKQVRLI
                     WKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            complement(10955..11509)
                     /locus_tag="RHZ76_002329"
     CDS             complement(10955..11509)
                     /locus_tag="RHZ76_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HDV2812962.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSTTCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGEDFLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYATGKMN"
     gene            11859..12062
                     /locus_tag="RHZ76_002330"
     CDS             11859..12062
                     /locus_tag="RHZ76_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV2812963.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            12099..12923
                     /gene="dapF"
                     /locus_tag="RHZ76_002331"
     CDS             12099..12923
                     /gene="dapF"
                     /locus_tag="RHZ76_002331"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDV2812964.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12920..13627
                     /locus_tag="RHZ76_002332"
     CDS             12920..13627
                     /locus_tag="RHZ76_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HDV2812965.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            13624..14520
                     /gene="xerC"
                     /locus_tag="RHZ76_002333"
     CDS             13624..14520
                     /gene="xerC"
                     /locus_tag="RHZ76_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDV2812966.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            14520..15236
                     /gene="yigB"
                     /locus_tag="RHZ76_002334"
     CDS             14520..15236
                     /gene="yigB"
                     /locus_tag="RHZ76_002334"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HDV2812967.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            15320..17482
                     /gene="uvrD"
                     /locus_tag="RHZ76_002335"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             15320..17482
                     /gene="uvrD"
                     /locus_tag="RHZ76_002335"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HDV2812968.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(17629..18393)
                     /locus_tag="RHZ76_002336"
     CDS             complement(17629..18393)
                     /locus_tag="RHZ76_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HDV2812969.1"
                     /translation="MAHQLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFAVQSGP
                     MLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            18763..19713
                     /gene="corA"
                     /locus_tag="RHZ76_002337"
     CDS             18763..19713
                     /gene="corA"
                     /locus_tag="RHZ76_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDV2812970.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPAAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(19755..20246)
                     /locus_tag="RHZ76_002338"
     CDS             complement(19755..20246)
                     /locus_tag="RHZ76_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HDV2812971.1"
                     /translation="MKSKLQKIILCLFILCCIYNLWTLRPVQVLYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGNLHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFKK
                     DNF"
     gene            complement(20243..20581)
                     /locus_tag="RHZ76_002339"
                     /pseudo
     CDS             complement(20243..20581)
                     /locus_tag="RHZ76_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
BASE COUNT         4831 a         5262 c         5322 g         5166 t
ORIGIN      
        1 aaatattcac tcaggaagtt attactcagg aagcaaagag gattacagaa ttatctcata
       61 acaagtgtta agggatgtta tttcccgatt ctctgtggca taataaacga gtagatgctc
      121 attccatctc ttatgttcgc cttagtgcct cataaactcc ggaatgacgc agagccgttt
      181 acggtgctta tcgtccactg acagatgtcg cttatgcctc atcagacacc atggacacaa
      241 cgttgagtga agcacccact tgttgtcata cagacctgtt ttaacgcctg ctccgtaata
      301 agagcaggcg tttttttata tgtcaggaag gccccggagg tgcttgcccc cgggtgagaa
      361 ggaactactg tggtgggttg ttctgcaacg ttaacatcaa accgtcgtga cgcatcgctg
      421 cagcttcttc cggcttgtgc agtctgtcca gcgcgtcggc aagccatgcg taatcgtagg
      481 cgtccggacg ttgtttcagc gctgcgcgga aggcgagcga tgcttcctgc cattctccgt
      541 gcttcatcag tgactggccc agtgtgctcc acaacagcgg gcgatcgccg acgtttttga
      601 tctgctggcg cagcactttt tccagctgtt ccggattgtt ggttttcagt cgaggaatcg
      661 gcagcagtag gcgatcgtcg tactggcgtt tcaggccatc gatgataatt tgctgggcag
      721 tatcatgatc gtcacattca ataagatgtt ccgccattgc cacctgcaac gctacctgat
      781 gacgcgtttt ccggctttgg tttttccacc agttacgcaa accttcacta ccgttatcgg
      841 cacgcgcctg atccatcagg ccaatccatg cctgttgttc cagcattgca cgatgttctt
      901 catcaccaac atgggctttc gccattgatg ggatgatatc cagcagagaa ctccatgcac
      961 ctgtgcggat atacgcctgt tccgccagac gtaatacttc cggatggcgt ggcgtaactt
     1021 ccagcagctt atccacgccg tggcgtgcag catggttttc attacgggcc agttgcagac
     1081 gtacacgggt gatttctacc ggaatggtgt cattgccggc cagctccgct gcgcgttcca
     1141 gatgttggtt ggcgcgcgct tcatcaccac gttgttgcgc ggcttcggca gccagtagat
     1201 agttcaccac cggttgttca gcgtgatcgg catttttcgc catcagcttt tcaacttgct
     1261 gataatcgcc ttccgccagt ttcagcagcg cctgttcggt ctgcttacgt gcacggcgac
     1321 gcttacgccc gacaaaccac ccacgggtgt gcgcgccagt gcggaagatc cgccgcagta
     1381 gccactcaat ggcaaacagc actaccatcg ccagaatcaa tatgatcgcc aggcccgtga
     1441 cgctggtttc gatattgtag ttgtcggtct ggatcagcac ataaccctga tgaccggcaa
     1501 tcatcgggcc aaccactatc cccgcaatca gcaacacaaa gagtaataac acttttagca
     1561 tggttattct ccttgcggcg cggctgccgg agtatcacct tgcggtgcag gtgcaggtgc
     1621 aggtgcaggt ttagcttccg ttgtccctgc tgccggttgt gccagcaggt tacgcacgcg
     1681 agtctgcatc agtttttcca gcatcgcctg gctttgcagg gtttccggaa gatccatcga
     1741 gatattttgc tggcttaact ggtccacctc gtcgaggaac gctttggtgg tggcatcatc
     1801 agtatcgtag taagcacgta cccaggtgga gacgttctcc agcgcctggc gataagtctc
     1861 ttcctggtga cgcggtacag cttgtgctgc gaccagcagg cgagagcgaa tattttcgcg
     1921 cagatagata tcctgatttg gcgctaacag cggtacggcg gtgtcatcac gacggcgaat
     1981 cgtaatgaag ttgtccataa agttctgcca gcttttttgc agattgatac gccattcgct
     2041 gatggaactg gaaagctctt caccgtctga atccatcggc gaaccatcgc tgtcattatc
     2101 ggccagacgc aggttatcta cctgatttga aagctgatta agcttaagga tgatgccgtc
     2161 ataatccacc tgcgatactg cagaaaggct ggcgatatca tcggtaattg cccgacgaac
     2221 ggtaatcaga ctcgggtcat tcatatccgc caggctggcg tctgcacttt tcagcaacgc
     2281 tgcagcggtc gtgacgtcct gatcgctcca cagcttccgt ccggcgagtt tcaccagaaa
     2341 atcggcctga gccagcagcc aggttttagc atcgctgccg gaaatggtgg cgaccttttg
     2401 ttggacttca tccaactgtt ttgccagcgt ttcttgctga cgattcgcct gcttaagttg
     2461 tgcagcttgt tgcttaataa tgccttccag ctcggctttt tggctctcct gggctttttg
     2521 caatgccgtc agttggttag ccagggcatc gctggtggcg gtctgattga cggcctgttg
     2581 tttaccccag ccatacaaac cgacgcccgc cgccagagca atagcgatag ccaccgcgct
     2641 gagaatcaat gcggtattgt tcttactctt tttttctgtt gcgacaggtt gtgacgtggt
     2701 gtccacggcc tccctggtct cttcaaccac ggcggaggtt ttttcttgtt ccgtcattat
     2761 ggcttcctgt tatgagagtt attgtaatgc ccgtaaaagc gcatcgttgt cagcgttatc
     2821 ggcgacctta atgtcttgcc agcccagttc ccgggcgagt ttcgccaaac gctcactgac
     2881 gaccaatagt cgacagtgta gtaaccagtg ctcacgatac cattgtggga tcagcgacca
     2941 gagttgctgc aacatttcac cgctggtaac aacgaccatc gtcacctcgc gggattgcca
     3001 gcgcatcgct tcttctgcac catcgtaatg gattgcgcat cgttgataac attcacaaaa
     3061 agtgacctca gcaccgcgcg ctgtcagggt atccccaatt agctcacgac cgccattgcc
     3121 acgtaatatc agcgcacgtt tgcccgcaat attttgtaat tcaggtaatt gtagcaagac
     3181 ttcgctgatt tcccgatcct gcgggtagag aatcttctgt ccgcttacgg tatgtagcgc
     3241 cagtgcggtg gtgcgtccaa tggcgaaata atcaggtagt cggggccatt tacgatcttg
     3301 ctgatgcagc tgtgattggg caaaagcaac cgcgtgttgc gagagggcaa acaacagatc
     3361 gctctccccc agcgccgcca gttgatcagc aagttgcggt aattgttgac ccggagaaaa
     3421 ctcaatcagc ggaaaatgcc aggccacctg ccccagtgtg cgcagacggc tcactaactc
     3481 ttctccagcg ggagacgggc gggtgaccag gatactcatg ccggggcgtc tccgttatag
     3541 acttcagcga ggatctcgcg cgcgccgtta ttcagtagct cttctgccag cgaaatcccc
     3601 atttgttcgg catcttgcgg cgcaccgcgg cgttcaccgc gaataatctg cgaaccgtcc
     3661 ggcgcgccga ccagcgcacg cagccagatt tcgccatcaa taagctcggc gtagctacca
     3721 attggcacct gacatccgcc ttcgagacgg gtattcatgg cgcgttctgc ggtaacgcgc
     3781 agtgcagttt cgtggtgatt cagcgcggca agcagctcgc gagtgcgtga atcatcaagg
     3841 cggcattcaa tacccaccgc accttgtcct accgccggaa gagaaatctc gggtggcaac
     3901 gcggcgcgaa tacgtgactc cagacctaaa cgttttagtc cggctacggc aagaatgatg
     3961 gcatcgtatt cgccgttatc cagtttgctc aggcgagtgc cgacgttgcc gcgcagggag
     4021 cggataatca gatccggacg gcgttcagcc agttggcact ggcgacgtaa actggacgtc
     4081 ccgacgatac tgcctgccgg taacgcatcc agactgtcat agttattgga cacaaaggca
     4141 tcgcgaggat cttcacgctc acaaatagtg accagtccca gaccttgcgg gaattcaacc
     4201 ggcacatctt tcattgagtg tacggcgata tcggcgcgat tttcgaggag cgcgacttcc
     4261 agctctttta caaataagcc ttttccgcct actttcgcca gcggcgtatc aagaatcaca
     4321 tcgccgcgcg tcaccatcgg taccagttca acgaccaggc ccggatggct cgccatcaac
     4381 ttgtctttga catagtgtgc ctgccagagt gcaagtgggc tttggcgtgt ggcaattctt
     4441 aaaacattgt ctaacatgct tgttaccgtc attatcatcc gtggtccatc ctaacatcct
     4501 tgccagagtg atgtcagtgt tgtggtgaaa cgtagacgcc tgcgcaaacc gtaaaatgag
     4561 gtctggcagt ggatcctgac aggcgtttca cgccgtcgta ataaggaatt tacagagaat
     4621 aaacggtgct acacttgtat gtagcgcatc tttctttacg gtcaatcagc aaggtgttaa
     4681 attgatcacg ttttagacca ttttttcgtc gtgaaactaa aaaaaccagg cgcgaaaagt
     4741 ggtaacggtt acctttgaca tacgaaatat cccgaatgcc gcgtgttacc gttgatgttg
     4801 gcggaatcac agtcatgacg ggtagcaaat caggcgatac gtcttgtacc tctatattga
     4861 gactctgaaa cagagactgg atgccataaa tcaattgcgt gtggatcgcg cgcttgctgc
     4921 tatggggcct gcattccaac aggtctacag tctactgccg acattgttgc actatcacca
     4981 tccgctaatg ccgggttacc ttgatggtaa cgttcccaaa ggcatttgcc tttacacgcc
     5041 tgatgaaact caacgccact acctgaacga gcttgaactg tatcgtggaa tgtcagtaca
     5101 ggacccgccg aaaggtgagc ttccaattac tggtgtatac accatgggca gcacctcgtc
     5161 cgtagggcaa agttgttcct ctgacctgga tatctgggtc tgtcatcaat cctggctcga
     5221 tagcgaagag cgccagttgc tacaacgtaa atgtagcctg ctggaaagct gggccgcctc
     5281 gctgggtgtg gaagtcagct tcttcctgat tgatgaaaac cgtttccgtc ataatgaaag
     5341 tggcagtctg gggggcgaag attgtggctc cacccagcat atactgttgc ttgacgaatt
     5401 ttatcgtacc gccgtgcgtc tcgccggtaa gcgtattctg tggaatatgg tgccgtgcga
     5461 cgaagaagag cattacgacg actatgtgat gacgctttac gcgcagggcg tgctgacgcc
     5521 aaatgaatgg ctggatctcg gtggcttaag ctcgctttct gctgaagagt actttggtgc
     5581 cagcctttgg cagctctaca agagtatcga ttccccatac aaagcggtac tgaaaacact
     5641 gctgctggaa gcctattcct gggaataccc gaacccacgt ctgctggcga aagatatcaa
     5701 acagcgtttg cacgacggcg agattgtatc gtttggtctc gatccatact gcatgatgct
     5761 ggagcgtgtt actgaatacc tgacggcgat tgaagatttt acccgtctgg atttagtacg
     5821 tcgctgcttc tatttaaaag tgtgcgaaaa gctcagccgt gaacgcgcct gcgtaggctg
     5881 gcgtcgcgca gtgttgagcc agttagtgag cgagtggggt tgggacgaag ctcgtctggc
     5941 aatgctcgat aaccgcgcta actggaagat tgatcaggtg cgtgaggcgc acaacgagtt
     6001 gctcgacgcg atgatgcaga gctaccgtaa tctgatccgc tttgcgcgtc gcaataacct
     6061 tagcgtctcc gccagtccgc aggatatcgg cgtgctgacg cgtaagctgt atgccgcgtt
     6121 tgaagcatta ccaggtaaag tgacgctggt aaacccgcag atttcacccg atctctcgga
     6181 accgaatctg acctttattt atgtgccgcc gggccgggct aaccgttcag gttggtatct
     6241 gtataaccgc gcgccaaata ttgagtcgat catcagccat cagccgctgg aatataaccg
     6301 ttacctgaat aaactggtgg cgtgggcatg gtttaacggc ctgctgacct cgcgcacccg
     6361 tttgtatatt aaaggtaacg gcattgtcga tttgcctaag ttgcaggaga tggtcgccga
     6421 cgtgtcgcac catttcccgc tgcgcttacc tgcaccgaca ccgaaggcgc tctacagccc
     6481 gtgtgagatc cgccatctgg cgattatcgt taacctggaa tatgacccga cagcggcgtt
     6541 ccgcaatcag gtggtgcatt ttgatttccg taagctggat gtcttcagct ttggcgagaa
     6601 tcaaaattgc ctggtaggta gcgttgacct gctgtaccgc aactcgtgga acgaagtgcg
     6661 tacgctgcac ttcaacggcg agcaatcgat gatcgaagcc ctgaaaacta ttctcggcaa
     6721 aatgcatcag gacgccgcac cgccagatag cgtggaagtc ttctgttata gccagcatct
     6781 gcgcggctta atccgtactc gcgtgcagca actggtttct gagtgtattg aactgcgcct
     6841 ttccagcacc cgccaggaaa ccgggcgttt caaggcgctg cgcgtttctg gtcaaacctg
     6901 ggggttgttc ttcgaacgcc tgaatgtatc ggtacagaaa ctggaaaacg ccatcgagtt
     6961 ttatggcgcg atttcgcata acaaactgca cggcctgtca gtgcaggttg aaaccaatca
     7021 cgtcaaatta ccggcggtgg tggacggctt tgccagcgaa gggatcatcc agttcttttt
     7081 cgaagaaacg caagacgaga atggctttaa tatctacatt ctcgacgaaa gcaaccgggt
     7141 tgaggtatat caccactgcg aaggcagcaa agaggagctg gtacgtgacg tcagtcgctt
     7201 ctactcgtca tcgcatgacc gttttaccta cggctcaagc ttcatcaact tcaacctgcc
     7261 gcagttctat cagattgtga aggttgatgg tcgtgaacag gtgattccgt tccgcacaaa
     7321 atctatcggt aacatgccgc ctgccaatca ggatcacgat acgccgctat tacagcaata
     7381 tttttcgtga tgaacgtgcc ggaaagcgag gcttatccgg catgcaatct tagcggaaac
     7441 tgactgtttc acccgcctgc tgcgtcgccg cctgttccag caaatcccag aaggtttcgc
     7501 cgctgcgatc gcaaatccac tcatcgcctt tcagatcaaa atggtagccg ccttgtttgg
     7561 ttgccagcca tacctggtgc agcggttcct ggcggttgat aatgattttg ctgccatttt
     7621 caaaggtaat ggtcagtacg ccgccgttga tttcgcagtc gatatcgctg tcgccatccc
     7681 agtagtccag gcgttcttca atggtcagcc agagttgatc agccaggcga tgaaattcac
     7741 tgtcgttcat tgttgtatcc tgtttttaag tgatggcggc agtatagcgg tatggggtca
     7801 gggcttcaaa gtttgcacct ctgctgctgc gttccggcat gattcatccg tcaccggaat
     7861 aatgatgtct ctgttttgaa cttcacgtac gttctcctct ctgacacgag ttttcgccgg
     7921 gcagggggag ggcgtaatcc ttacctctgt atcggtccat gtataaacat tgcgatcgtc
     7981 ataagcgaag cgatcgtttt tagttagcgg accgttaact ggaataattt gcaagctctt
     8041 tgaccgtaat tggcggataa agtaagcatg ctgtttgccg ctattaaatg agcgatcaat
     8101 gaccaggtct ggcgtatcca gcgggattat ctcaagctgg tcttcaccaa atgtcgataa
     8161 ttttgttaca tacagccgat ctttatattg cgcaatattg ccagtaatca gatgaaattg
     8221 ttcaggttca atacctggta ggtctatcca cttgctccac tgacggtcgg gcgtgagctg
     8281 tttacgccag cgaacgcgac tttcccctac ttcaaaagag acgtcatcat ctacgggtat
     8341 cgctttgcct gataattgac caaagggata acgatgcaga cccttattgt cgcgaaaaac
     8401 cagcagtgaa tgaggtatcc agcgaattat ctcaaatgag tctgcattcg ccgtaaggtg
     8461 aacgccattt aaataaatat tctgcccact gcggataagc agatcattgg aatacgaatt
     8521 tctgtttaca ctcataagac gtggattaat accccaccat cttttttcta atacagtaac
     8581 atcactgctg ctcccctgac gtcgaccgga caagtagaga tttttactat ccatcagcgt
     8641 ggtagagtta ccttcgatag cttttagtgt cgccagatca acacgtgacg cgccgctatt
     8701 actttcggtg cgctgtccgt caaaatagag gctatattta tcgaccgcga agcggcccat
     8761 agcctgaaag ctggcaatat cgacagtggg ggttcccggt ggattgctaa cgattttgcc
     8821 acgccagaga ataacgcggc cgtctgtcac ccagtggtat tcctcatttt ttatgcttat
     8881 ttcatcggtg ggtatatagc cttccaccaa ctcccgctcc ccggatatat ttgtcacaag
     8941 gcgcaaatcg gctctgttca tcttcggaaa ttgggcaatg attttctcat tctccccata
     9001 acctttaccg taagacataa caccgtgatc attgtaatag tcgaacccta tggcctgtac
     9061 agcaggcgtc agaaatatgt gccccaacag gatgagtgca actgtgatta tttgaccatt
     9121 cattttgtcg cctcattgcg cggggcagga ataaattcgc cgtcatcagt aatttttccg
     9181 tccagatatc ccgttttgta atttacgcca tagccatatc ccgaatgttg ttccttccag
     9241 attaagcgaa cttgtttcgg atcagcggtg cggtatcgcc agagctgcgc gccatcaaag
     9301 ctgtaaacgt agcggttatc gcgggcatac catctgcgca aaaagggatg gtcattaaga
     9361 tgggcttcgc gttcatccgg agagtggtta tccagtaaga tcagcggtcc gtcagattca
     9421 aacacgtgta aaccggaatg gtatggccac ccttcggcac gcgtaagtaa atagccgtgg
     9481 cgtttcccgg cgttgaaacg tttgttgata ataagattgg gatcatcaag tttcacgatc
     9541 tccagcttgc ggtcaccaga ctctgtttcg atgatggtat aaagcgagtc ctgatactgg
     9601 gcgacagcat cactgatggg gtgaaactgt tccggatcga gtccgggtaa ttcttcctgt
     9661 ttacaagcag gccctttacg ccagaacact ttcttttcca gcagagaaaa atccagacag
     9721 tgctttgtta aatcttcatc ccacgccaga ttttccttgt cgaggacttt acgctgtagc
     9781 ccgtttttat cacgccaggt caagagtgag ccgggcatcc agcgtacgac ggaaaaggtg
     9841 ttcgggtcgg catcaagctg ttcgctgttg atcaggattt tggtccgtgt acgagccagg
     9901 gtatcccagg ggccaaatgg ggcaggaagg cagggattga atgctgtaga gaatttaccg
     9961 cgctgatccc atgatttttg tgccagtacc gtaaaggtgt caggatcatc aaggcgctga
    10021 ccatttgcgt aaagataatg cttgtcacgg agaatcaaac ctagccagtc tggtttccat
    10081 tgcgggttaa attctacctg acgcaacgtt cggcggttta gttgctggtt gctatcggtg
    10141 cgtttgcctt cgtaatagag gctgtactta tctgcggcaa aatcacccca tgcctgaaat
    10201 gacgagatat cgactggcgg tgtgcctgga gggttttgca caatctcgcc agcgtagaga
    10261 atagcgcgac catcgctata gtaagagtaa tggggaagag ttgcgtttgc gccgctgtag
    10321 acaacgtctg tcgtgagatc agtagccggg ggcaccgttt cccaatcctt cagttttgtt
    10381 gggtcaatta acaaattcgg taacagacgc agacgcttgt agttaatctg cggtagcgaa
    10441 agattggtct tttcataagg gcgcaggtac accacgcctt ttccttgttt ctggtaaata
    10501 ttatgttccg gctcatcaag ccgacatccc catgccgatg ataagaacaa tgaacaagac
    10561 aacaaacaaa ccacattgcg atagtgcata aagccatcct ggcgcgaggt gccgatcacg
    10621 aaactaccag cagaacataa ttccccgcac gtaagcgtaa cttttgcggc aaatcgtcac
    10681 ttttcagaat ccataaaggt cattgctatt ttctgatagt tgctgggtaa acgtgatgtt
    10741 tccattcgcg atataaatac gaggtactgc gccatggtgt gactgatcac gttaaaatat
    10801 tccggattga cgccgggaag ggtttcaaca tgcggctctg cccccgttgc ctactttatt
    10861 gtctttcgcg agttgtaccc aggtcccacc atcggtatct aacattcccc acattattac
    10921 gctttctgcc gaacgtgatt tttatagaaa tctatcaatt catttttcct gtggcataaa
    10981 gtttatcttt cacatcctgc cgaacgcggg tcttgtaccc cccattgtga agatcttcat
    11041 acggagaaaa agaatcttta ctccacccca gcagcacttt atcgccaggc ataaaatgca
    11101 catgcagaaa atcttctccc cattctcttt tggggagagg taacgtcata ttccgtttct
    11161 ccagacgcat tccagcatta tactgtgtac gagtagtact taatgtccag ataacctctg
    11221 ccgtatcccc actaacatca ccacaacagg ttgttgaacc gccaccattt gcaaaagcat
    11281 tcgcgcccgc cacgccgtta acactgaatc ccagtatcgg gcggtcaatt tcactgtgga
    11341 tttccagaat cactccacct cttggtcctc cccatactgc ctgtcctata ttccacaaca
    11401 cgaccagtac cggaacgaca attaacgtcc gccataccca gcgcccccac cgggcataac
    11461 cgcgcgtaag cttttggtca aatttttcca gttgggtaaa tagccccatc agattttatt
    11521 ccctcttatc accaggttgg caaagttgta tgtcactggc gactaaggtc agacaacaaa
    11581 attgcatggt gcgccactct tttaaaagct ccatgccgac gataagaaca acgaacaagc
    11641 caacaaacaa accacattgc gatagtgcat aaagccatcc tggcgcgagg tgttgatcac
    11701 gaaactgcca gcagaacata aatcctcacg agtaagcgtt atactcgcag catttcctca
    11761 cttttcagac ttcataaaga gtcgctaaac gcttgctttt acgccttctc ctgcgatgat
    11821 agaaagcaga aagcgatgaa ctttacaggc aatccataat gaaaaacgtg tttaaggcac
    11881 tcactgtatt acttactctc ttcagcctga cgggctgcgg tctgaaaggt ccgctctatt
    11941 tcccgccagc agataaaaac gcaccgccgc cgaccaaacc ggtagagacg caaacgcaat
    12001 ccacggtgcc ggataaaaac gatcgcgcca ctggcgatgg tccatcccag gtgaattact
    12061 aaaagtcagt ttctgtaccc gcgtgattgg agtaaatgat gcagttctcg aaaatgcatg
    12121 gccttggcaa cgattttatg gtcgtcgacg cggtaacgca gaatgtcttt ttttcaccgg
    12181 agctgattcg tcgcctggct gatcggcacc tgggggtagg gtttgaccaa ctgctggtgg
    12241 ttgagccgcc gtatgatcct gaactggatt ttcactatcg cattttcaat gctgatggca
    12301 gtgaagtggc gcagtgcggc aacggtgcgc gctgctttgc ccgttttgtg cgtctgaaag
    12361 gactgaccaa taagcgtgat atccgcgtca gcaccgccaa cgggcggatg gttcttaccg
    12421 tcaccgatga tgatctggtc cgcgtaaata tgggcgaacc caacttcgaa ccttccgcgg
    12481 tgccatttcg cgctaacaaa gcggaaaaga cctatattat gcgcgccgcc gagcagacaa
    12541 tcttatgcgg cgtagtgtcg atgggaaatc cacattgcgt gattcaggtc gatgatgtcg
    12601 ataccgcggc ggtagaaacg cttggtcctg ttctggaaag ccacgagcgt tttccggagc
    12661 gcgccaatat cggttttatg caagtggtta agcgcgagca tattcgttta cgcgtttatg
    12721 agcgtggggc aggagaaacc caggcctgcg gcagcggcgc gtgtgcggcg gttgcagtag
    12781 ggattcagca aggtttgctg gccgaagaag tacgcgtgga actccccggc ggtcgtcttg
    12841 atatcgcctg gaaaggtccg ggtcacccgt tatatatgac tggcccggcg gtacatgtct
    12901 acgacggatt tattcatcta tgaagcaacc aggggaagaa ctgcaggaaa cactcacgga
    12961 gcttgatgac cgggcggttg tcgattatct gattaaaaat cctgagtttt ttatccgtaa
    13021 tgcgcgcgca gtagaagcga tacgtgtgcc gcatccggta cgcggcaccg tttcgttggt
    13081 cgagtggcac atggcccgcg cacgtaatca tattcatgtt ctggaagaga acatggcgct
    13141 gttgatggaa caggctatcg ccaacgaagg cctgttttat cgcctactct acctgcagcg
    13201 cagtctcacc gccgccagca gtctcgacga tatgctgatg cgctttcacc gctgggcgcg
    13261 cgatctcggc ctggcaggtg cgagtctgcg cctgtttccg gatcgctggc gcttaggtgc
    13321 gccgtcgaac cacactcatc tggcattaag ccgtcagtct ttcgaaccgc tgcgtattca
    13381 gcgtttgggg caggaacagc actatcttgg gccgcttaac ggaccagagc tgctggtggt
    13441 gctaccggaa gcgaaagcgg tgggatcggt ggctatgtcg atgctgggaa gcgatgctga
    13501 tttgggtgtc gtgctgttta ccagtcgcga tgccagtcac tatcaacaag ggcagggaac
    13561 gcagttactt catgaaattg cgctgatgtt gccggagctt ctggagcgtt ggattgaacg
    13621 cgtatgaccg atttacacac cgatgtagaa cgctacctac gttatctgag cgtggagcgc
    13681 cagcttagcc cgataaccct gcttaactac cagcgtcagc ttgaggcgat catcaatttt
    13741 gccagcgaaa acggcctgca aagctggcaa caatgcgatg cagcgatggt acgcaatttt
    13801 gctgtacgca gtcgccgtaa agggctggga gcagcaagtc tggcgttacg gctttctgcg
    13861 ctacgtagct tttttgactg gctggtcagc cagaacgaac tcaaagctaa cccggcgaaa
    13921 ggtgtttcgg caccgaaagc gccgcgtcat ctgccgaaaa atatcgacgt cgacgatatg
    13981 aatcggctgc tggatattga tatcaacgat cccctcgccg tgcgcgaccg ggcaatgctg
    14041 gaagtgatgt acggcgcggg cctgcgtctt tccgaactgg tggggctgga tatcaaacac
    14101 ctcgacctgg agtccggcga agtgtgggtg atggggaaag gcagcaaaga gcgccgcctg
    14161 ccgattggtc gcaacgctgt ggcgtggatt gagcactggc ttgatttgcg cgacctgttt
    14221 ggtagcgaag acgacgcgct ttttctgtcg aaactgggca agcgtatctc cgcgcgtaat
    14281 gtgcagaaac gctttgccga atggggcata aaacaagggc tgaataatca cgttcacccg
    14341 cataaattac gtcactcgtt cgctacgcat atgctggagt cgagcggcga tcttcgtggt
    14401 gtgcaggagc tgctgggtca tgccaacctc tccaccacgc aaatctatac tcatcttgat
    14461 tttcaacacc ttgcctcggt gtacgatgcg gcgcatccac gcgccaaacg ggggaaataa
    14521 tgcgttttta ccggcctttg gggcgcatct cggcgctcac ctttgacctg gatgataccc
    14581 tttacgataa ccgtccggtg attttgcgca ccgagcgaga ggcgcttacc tttgtgcaaa
    14641 attatcatcc ggcgctgcgc agcttccaga atgaagatct gcaacgcctg cgccaggcgg
    14701 tacgggaagc ggaacccgag atttatcacg acgtgacgcg ctggcgtttt cgttcgattg
    14761 aacaagcgat gctcgacgcc gggctgagtg ccgaagaagc cagtgccggc gcacacgcag
    14821 caatgatcaa ctttgccaaa tggcgcagcc ggatcgacgt cccgcagcaa acccacgaca
    14881 ccttaaaaca gctggcgaag aaatggccgc tggtggcgat caccaacggt aacgcccagc
    14941 cggagctgtt tggtttgggg gattattttg agtttgtgct gcgcgctggt ccgcacgggc
    15001 gctcaaaacc gttcagcgat atgtactttc tggcggcgga aaaactcaac gtgccgatcg
    15061 gcgagatctt acatgttggg gacgatctca ccactgacgt gggtggggca attcgcagcg
    15121 gaatgcaggc ttgttggatc agaccggaaa atggcgatct gatgcaaacc tgggacagcc
    15181 gtttactgcc acatctggaa atttcccggt tggcatctct gacctcgctg atataatcag
    15241 caaatctgta tatataccca gctttttggc ggagggcgtt gcgcttctcc gcccaaccta
    15301 tttttacgcg gcggtgccaa tggacgtttc ttacctgctc gacagcctta atgacaaaca
    15361 gcgcgaagcg gtggccgcgc cacgcagcaa ccttctggtg ctggcgggcg cgggcagtgg
    15421 taagacgcgc gtactggtgc atcgtatcgc ctggctgatg agcgtggaaa actgctcgcc
    15481 atactcgatt atggcggtga cgtttaccaa caaagcggcg gcggagatgc gtcatcgtat
    15541 cgggcaactg atgggcacca gccagggcgg tatgtgggtc ggcaccttcc acgggctggc
    15601 gcaccgcctg ctgcgtgcgc accatatgga cgccaatctg ccgcaggatt tccagatcct
    15661 cgacagtgaa gaccagctac gcctgcttaa gcgtttgatc aaggcgatga acctcgacga
    15721 gaagcagtgg ccgccgcgcc aggcaatgtg gtacatcaac agccagaaag atgaaggcct
    15781 gcgtccgcat catattcaaa gctacggtaa tccggtggag cagacctggc agaaggtgta
    15841 tcaggcgtat caggaagcgt gcgatcgtgc gggtctggtg gacttcgccg agctgctgct
    15901 gcgcgctcac gagttgtggc ttaacaagcc gcatatcctg caacactacc gcgaacgttt
    15961 taccaatatc ctggtggacg aattccagga taccaacaac attcagtacg cgtggatccg
    16021 cctgctggcg ggcgacaccg gcaaagtgat gatcgtcggt gatgacgacc agtcaatcta
    16081 cggctggcgc ggggcgcagg tggagaatat tcagcgtttc cttaatgatt tccccggtgc
    16141 cgaaactatt cgtctggagc aaaactaccg ctctaccagc aatattctga gcgccgctaa
    16201 cgccctgatt gaaaacaata acgggcgtct gggtaaaaaa ctgtggaccg atggcgcgga
    16261 cggtgagcct atttccctct attgcgcttt taacgaactc gatgaagcgc gttttgtggt
    16321 taaccgcatc aaaacctggc aggacaacgg cggagcgctt gccgagtgcg ccattctcta
    16381 ccgcagcaac gcccagtcgc gggtgctcga agaggcgtta ttgcaggcca gtatgccgta
    16441 ccgtatttac ggcgggatgc gcttcttcga acgccaggaa atcaaagatg cgctctcgta
    16501 tctgcgcctg attgccaacc gcaacgacga cgcggccttt gagcgtgtgg tgaatacgcc
    16561 aacgcggggt attggtgacc ggacgctgga cgtggtacgt cagacatcgc gcgatcgcca
    16621 gttaacactc tggcaggcat gtcgtgagct gttgcaggaa aaagccctcg ccgggcgagc
    16681 tgccagcgcc ttgcagcgat ttatggaatt aatcgacgcc ttagcgcagg aaactgccga
    16741 tatgccgctg catgtacaga ctgaccgggt aattaaagac tccggcctgc gtaccatgta
    16801 tgagcaggag aagggcgaaa aaggtcagac gcgtatcgaa aacttagagg aactggtgac
    16861 ggcaacgcgc cagttcagct acaacgaaga agacgaagat ttaatgccgc tgcaggcgtt
    16921 cctctcccat gcggcactgg aagcaggtga agggcaggcg gatacctggc aggatgcggt
    16981 gcagttgatg acgctacact cggcgaaagg cctggagttc ccgcaggtgt ttatcgttgg
    17041 tatggaagag ggcatgttcc caagccagat gtcgctggat gaaggcgggc gtctggaaga
    17101 agaacgccgt ctggcctacg ttggcgtaac ccgcgcgatg cagaaactga cgctgaccta
    17161 cgcggaaacc cgccgtctgt atggtaaaga ggtttaccat cgcccgtcgc gctttatcgg
    17221 cgagctgccg gaagagtgtg tggaagaggt gcgcctgcgc gccacggtaa gccgcccggt
    17281 cagccatcag cggatgggta cgccgatggt cgagaacgac agcggctaca agctcggcca
    17341 gcgcgtacgc cacgctaagt ttggtgaagg caccattgtc aatatggaag gcagcggtga
    17401 gcatagccgt ttgcaggtgg catttcaggg ccagggtatt aaatggctgg tggcggcata
    17461 cgcccggctg gagtcggtgt aacgttgccg gatgcggtgc tgcgcacctt atttggccta
    17521 aaaaatcatt catattcaat aaattgcaac gtcatgtatg ccggataggg cgtttacgcc
    17581 gcatccggca tctgcgccat cttcagtatc tgacacaaaa ctatcgtttt aacctttccg
    17641 ctcgacggaa atcatggtga caaaaggata gcgttgccac gggattgccc cgcctttcat
    17701 atacatatgt gaaatcgtac catcaagata aagcagctgc tcaacgttca gtttcgcttt
    17761 ggcataacag gcaaaatcat aaaaatttgt cgcctgctgg ctcaacaaaa acacggcatt
    17821 cccatgttta ttaatcccaa caccgttacg aattttacgt gaggcgacgt tgggatgaat
    17881 acgcggatta attacaccgt tttccatcaa cattggccct gactgcaccg caaactgaat
    17941 atctttactg gctttaaaag catctagacg aacgatgccg actttatctc ccgcgacata
    18001 aaacacgccg ccaggacgga taaagaaatt cccttcacct gaagcgagat ttaacgccac
    18061 cttctgctga ccgttttcga tgtacaaacc gagcggcgca tagctttcat catagatgcc
    18121 gccgttcatc gccatctgca cctgaccctg actattaata tccgccagca gagcatgtaa
    18181 cgttccccac gcttcgccat tggctttttg ccagtacatt ttcacccgct ctgtttgagg
    18241 attaacggta tacgcctgta cggtcagcgt cggatctgag agtgcgcaat catcagcggc
    18301 aacagcaaac aagggaagta aggtgagggc gagaaaaatc cgtttgagat tcaaggtgat
    18361 cattccttta ccaatgagta gctgatgcgc cattataggt cctggatgtg ggattttttt
    18421 atcctgttag cgaccttgac gagtaccaaa aagcgcgaag ttcaactatt gttctgtggt
    18481 gttctgttgc gtgttgacgg caaaattttg ctggcgtaac atgcgcgcac gatcactcta
    18541 agaggacatt cgccttggac acacccagta gatactggct cactatcctg tcatccagga
    18601 tcaactccta aggctatccc tttttgctga tagccttagc ggttgtcagc gacctcaatt
    18661 tttcccgtcg cgctgagtca ggctgtttaa tggtctgaaa cccaatttgt ttctgtgtgc
    18721 ccaccgaact gtccgatatt ttaagcattg ggagtcccgg tcatgctgag cgcatttcaa
    18781 ctggaaaata accgactgac ccggctggaa gtcgaagagt cacaacccct tgtaaatgca
    18841 gtatggattg atcttgtcga accggacgac gacgagcgac tgcgcgtaca atctgaactt
    18901 ggccagagcc tggcaacccg cccggaactg gaagacatcg aagcatcggc acgtttcttt
    18961 gaagacgacg acggcctgca tattcactcc ttcttcttct ttgaagatgc ggaagatcac
    19021 gccggtaact ccactgtggc atttaccatc cgtgatggtc gtctgtttac tctgcgtgag
    19081 cgtgaactgc ccgcttttcg tctgtatcgt atgcgtgccc gtagccagtc gatggtagac
    19141 ggtaacgcct acgagttgct gctggatctg ttcgaaacca aaatcgaaca gttggcagat
    19201 gaaattgaaa atatctatag cgacctggag cagttgagcc gggtgattat ggaagggcat
    19261 cagggcgatg agtacgacga ggcgctctcc actctggcgg aactggaaga tatcggctgg
    19321 aaagttcgcc tgtgtctgat ggatacccag cgcgcgctca acttcctggt gcgtaaagcg
    19381 cgtttaccgg gtgggcaact ggagcaggcg cgtgaaatcc tgcgagatat cgaatccctg
    19441 ctgccgcata acgaatccct gttccagaag gtgaacttcc tgatgcaggc ggcaatgggt
    19501 tttatcaaca tcgagcagaa ccgcatcatc aaaatcttct cggtggtatc cgtggtattc
    19561 ctgccgccga cgctcgttgc ttccagctat ggcatgaact ttgagtttat gccagaactg
    19621 aagtggagct tcggctaccc tgccgcgatt atctttatga tcctcgcggg cctggcaccg
    19681 tatctgtact ttaagcggaa gaactggttg taatatgagt gccggataaa gctatttttt
    19741 tatccggctt atttctaaaa attgtctttt ttaaagtcat gcggattgaa aactctctct
    19801 attttatttt cttgtgttaa ttgatactca gtaaaatcgt taataccaat atgagtgtta
    19861 cgataagggt attcattaat aaccattaag taatttttca cgatgcaatt atcattgctc
    19921 ttaatcgtcg ggaaacagta aagatcttca tacggccctt cgatatattt tttatagtca
    19981 gtgaagccat taccgatatc aatgacatac caggtatgca aattaccttc tggtagggga
    20041 tgttgatttt taatttcatt ctgatgcttc aggtaccaat ttattttatc gctgtcagtc
    20101 catggcagat gatcaacgac cagaaagact gaattgccag catcagagta tgtatacaaa
    20161 acctgtacag gacgcaatgt ccataaatta tagatgcagc atagaataaa taaacataat
    20221 ataattttct ggagtttgct tttcatagat aattttcgat tctttcgata gtgttaaaat
    20281 tggtgaaaaa aggtttgaaa gcaaagtctt tatgccgttg taaaaagaac catatgcgaa
    20341 aaatctatat ttgctgtaag tcttgttttt gatatcagtt acatcaagac cgaagtggtc
    20401 ctgtgctaca aaatgaattg tcgcactcca tcctatagcg tatttttgaa agcttaaaag
    20461 agatattttt tgagctgaaa tatcatgaac actgattcct aagccattaa aactgtcctc
    20521 gaagtcatta aatttattta gtcttgatga aagtaatttg gttttaatgc tttgcaggat
    20581 a
//
LOCUS       DAPHBJ010000073        20078 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.073, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000073 DAPHBJ010000000
VERSION     DAPHBJ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.073"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(127..873)
                     /gene="cutC"
                     /locus_tag="RHZ76_002340"
     CDS             complement(127..873)
                     /gene="cutC"
                     /locus_tag="RHZ76_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDV2812972.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(887..1453)
                     /locus_tag="RHZ76_002341"
     CDS             complement(887..1453)
                     /locus_tag="RHZ76_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV2812973.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1669..3402
                     /gene="argS"
                     /locus_tag="RHZ76_002342"
     CDS             1669..3402
                     /gene="argS"
                     /locus_tag="RHZ76_002342"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDV2812974.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            3579..4067
                     /locus_tag="RHZ76_002343"
     CDS             3579..4067
                     /locus_tag="RHZ76_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HDV2812975.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DVNVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4187..4579)
                     /gene="flhe"
                     /locus_tag="RHZ76_002344"
     CDS             complement(4187..4579)
                     /gene="flhe"
                     /locus_tag="RHZ76_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HDV2812976.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4579..6657)
                     /gene="flhA"
                     /locus_tag="RHZ76_002345"
     CDS             complement(4579..6657)
                     /gene="flhA"
                     /locus_tag="RHZ76_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDV2812977.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NIVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGHEQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6650..7798)
                     /gene="flhB"
                     /locus_tag="RHZ76_002346"
     CDS             complement(6650..7798)
                     /gene="flhB"
                     /locus_tag="RHZ76_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HDV2812978.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDVVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8000..8644)
                     /gene="cheZ"
                     /locus_tag="RHZ76_002347"
     CDS             complement(8000..8644)
                     /gene="cheZ"
                     /locus_tag="RHZ76_002347"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HDV2812979.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8655..9044)
                     /gene="cheY"
                     /locus_tag="RHZ76_002348"
     CDS             complement(8655..9044)
                     /gene="cheY"
                     /locus_tag="RHZ76_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HDV2812980.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9059..10108)
                     /gene="cheB"
                     /locus_tag="RHZ76_002349"
     CDS             complement(9059..10108)
                     /gene="cheB"
                     /locus_tag="RHZ76_002349"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HDV2812981.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10111..10971)
                     /gene="cheR"
                     /locus_tag="RHZ76_002350"
     CDS             complement(10111..10971)
                     /gene="cheR"
                     /locus_tag="RHZ76_002350"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDV2812982.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10990..12591)
                     /gene="tap"
                     /locus_tag="RHZ76_002351"
     CDS             complement(10990..12591)
                     /gene="tap"
                     /locus_tag="RHZ76_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HDV2812983.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESVQLQIAPVVS"
     gene            complement(12637..14298)
                     /gene="tar"
                     /locus_tag="RHZ76_002352"
     CDS             complement(12637..14298)
                     /gene="tar"
                     /locus_tag="RHZ76_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HDV2812984.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14443..14946)
                     /gene="cheW"
                     /locus_tag="RHZ76_002353"
     CDS             complement(14443..14946)
                     /gene="cheW"
                     /locus_tag="RHZ76_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDV2812985.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14967..16931)
                     /gene="cheA"
                     /locus_tag="RHZ76_002354"
     CDS             complement(14967..16931)
                     /gene="cheA"
                     /locus_tag="RHZ76_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDV2812986.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16936..17862)
                     /gene="motB"
                     /locus_tag="RHZ76_002355"
     CDS             complement(16936..17862)
                     /gene="motB"
                     /locus_tag="RHZ76_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HDV2812987.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17859..18746)
                     /gene="motA"
                     /locus_tag="RHZ76_002356"
     CDS             complement(17859..18746)
                     /gene="motA"
                     /locus_tag="RHZ76_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HDV2812988.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGVGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLATVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18873..19451)
                     /gene="flhC"
                     /locus_tag="RHZ76_002357"
     CDS             complement(18873..19451)
                     /gene="flhC"
                     /locus_tag="RHZ76_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HDV2812989.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19454..19804)
                     /gene="flhD"
                     /locus_tag="RHZ76_002358"
     CDS             complement(19454..19804)
                     /gene="flhD"
                     /locus_tag="RHZ76_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HDV2812990.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4776 a         5540 c         4916 g         4846 t
ORIGIN      
        1 gctcaacaga tcacggttat cgttcgtttt ttatactgct cagggataaa aaaaggcccc
       61 tgttgaaatt gcaggggcct ggtacgagca agcatcatat tgggcgacat gatgcaacgg
      121 taaaaatcat ttggcctgat ggcgttcaat gattcctttc atttcagcaa ccgccgcccc
      181 gtctacgaca taacgcgaat actcgtccgc gtgtgcatct gatgacatag acaatccttg
      241 attacgataa cgcatcggtg aggcttgcca cgctcccgcg gagctatgga cttccagcac
      301 tccggcatcg aggaagtggt gcaggttttc tgcacggact cctgctccgg ccataatgat
      361 tggagcatca cgatgggcaa taagttccat aatttttgat aaaccttgca gtgcgtctga
      421 tttttgccct gatgtcagca cccgggcaat gcccaattcc gcaagattat tgagtgtatt
      481 tagagggtta gcgcacatat caaaggcgcg atgaaaagtc actgccagcg gaccggcagc
      541 agccattatt ttttccattc gtggcatatc gacattcccg tcaacatcga gaacgcccgt
      601 caccagtcca ggaaaaccta attcgcggac cgtgcgcaca tcctcaagaa tggcggcaaa
      661 ctcaccgtcg ctgtagcaaa aatcaccacc gcgtgggcga attatcggat gcacagggat
      721 cgtcacccgc tggcgcacgg atttcagtac acccaacgac ggcgttaagc ccccctcttt
      781 tggggctgcg cataattcaa ctctgtctgc gccgttttgc tgcgccgtta gtgcacattc
      841 catgctgtaa cagcaaattt ccagtaatgc catttttact ccttaattac gccgactgct
      901 cgctggcaac gatctcttca atggaccacg gatgaaactt aatagttgtt ttgccatcgg
      961 tgaccgccag cgtcggattc ggtaatcgct catgctcacc ttttggcgaa ctggttttca
     1021 ctgagatccc cggtaggctg agtccttcat cagaaagcaa tgccagcgca cgggcattca
     1081 gcgtttctgg atcacccggc agaacgattt caatatgttc ccaaccttcg tgtgggtaac
     1141 gtttttcccc gggccacggt agctccacaa tagaaaactg ccagtgcgca acctgtaccg
     1201 gttcatgcaa tttaaacaga caaatcggtc tgccattgat catattttct gacaaaagct
     1261 cgccacactg ttcaaacccg cgacgccagc gttcagcagt ggcgttttga tggcaacgca
     1321 aagaaatgtg atcggcagtc agcggagtga tattcaaccc cagacggcgg gaaagttcat
     1381 ctaatgcgtg ggtaaatcgc ggtaaatccg atgcaatatc ctgcagctcg tcgatagatt
     1441 gccagttcgc cataatcact cttcgtcttt cagtaaaagc gttaatttac cctgttgccc
     1501 tgtgccaacc aaccgctgat ttcacgccgc ttctgatgca atagtgaaaa cggcaatacg
     1561 ccacgcgcac gttgctgacg aaaacagcca tttgcagtat actcccgccc taatttcttt
     1621 aactggtgcg ggcaattttt gctcgcttca tcaatgtaag gtattccggt gaatattcag
     1681 gctcttctct cagaaaaagt ccgtcaggcc atgattgcgg caggcgcgcc tgcggattgc
     1741 gaaccgcagg ttcgtcagtc agcaaaagtt cagttcggcg actatcaggc taacggcatg
     1801 atggcagttg ctaaaaaact gggtatggca ccgcgacaat tagcagagca ggtgctgact
     1861 catctggatc ttaacggtat cgccagcaaa gttgagatcg ccggtccagg ctttatcaac
     1921 attttccttg atccggcatt cctggctgaa catgttcagc aggcgctggc gtccgatcgt
     1981 ctcggtgttg ctacgccaga aaaacagacc attgtggttg actactctgc gccaaacgtg
     2041 gcgaaagaga tgcacgtcgg tcacctgcgc tctaccatta ttggtgacgc agcagtgcgt
     2101 actctggagt tcctcggtca caaagtgatt cgcgcaaacc acgtcggcga ctggggcact
     2161 cagttcggta tgctgattgc atggctggaa aagcagcagc aggaaaacgc cggtgaaatg
     2221 gagctggctg accttgaagg tttctaccgc gatgcgaaaa agcattacga cgaagatgaa
     2281 gagtttgccg agcgtgcacg taactacgtg gtaaaactgc aaagcggtga cgaatatttc
     2341 cgcgagatgt ggcgcaaact ggtcgacatc accatgacgc agaaccagat cacctacgat
     2401 cgtctcaacg tgacgctgac ccgtgatgac gtgatgggcg aaagcctcta caacccgatg
     2461 ctgccaggaa ttgtggcgga tctcaaagcc aaaggtctgg cagtagaaag tgaaggcgcg
     2521 accgtcgtat tccttgatga gttcaaaaac aaggaaggcg aaccgatggg cgtgatcatt
     2581 cagaagaaag atggcggcta tctctacacc accactgata ttgcctgtgc gaaatatcgt
     2641 tatgaaacac tgcatgccga tcgcgtgctg tattacatcg actcccgtca gcatcaacac
     2701 ctgatgcagg catgggcgat cgtccgtaaa gcaggctatg taccggaatc cgtaccgctg
     2761 gaacaccaca tgttcggcat gatgctgggt aaagacggca aaccgttcaa aacccgcgcg
     2821 ggtggtacag tgaaactggc cgatctgctg gatgaagccc tggaacgtgc acgccgtctg
     2881 gtggcagaaa agaacccgga tatgccagct gacgagctgg aaaaactggc taacgcggtt
     2941 ggtattggtg cggtgaaata tgcggatctc tccaaaaacc gcaccacgga ctacatcttc
     3001 gactgggaca acatgctggc gtttgagggt aataccgcgc catacatgca gtatgcttac
     3061 acgcgtgtat tgtccgtgtt ccgtaaggca gaaattaacg aagagcaact ggctgcagct
     3121 ccggtaatca tccgtgaaga tcgtgaagcg caactggcag ctcgcctgct gcagtttgaa
     3181 gaaaccctca ccgtggttgc ccgtgaaggc acgccgcatg tgatgtgtgc ttacctgtac
     3241 gatctggctg gtctgttctc tggcttctac gagcactgcc cgatcctcag cgcagaaaac
     3301 gaagaagtgc gtaacagccg tctgaaactg gcacaactga cagcgaagac gctaaagctg
     3361 ggtctggata cgctgggtat tgagacagta gagcgtatgt aatcgatttt tcgtgagagt
     3421 aaagcctgat cagggttacc tgatcaggct tttttattgc catctaaatg tattctgaaa
     3481 atggacatgc cattgttttc tcactgttgg ataagaggcc agaagcgtaa tatccggccc
     3541 cagggaaacg ataacggttg aatttaagga ataccgcagt gtttaaattt cttgtattaa
     3601 cattaggcat tatctcttgc caggcttacg cagaagatac ggttatagta aacgaccatg
     3661 acatttcagc catcaaagat tgttggcaaa aaaattcaga tgatgatact gacgttaacg
     3721 tgatcaaatc atgcctgcga caagaataca atctcgtcga tgcgcaatta aataaagcct
     3781 atggtgaagc ttatcgttat atagaacaag tgccacgcac aggtgtaaaa aaacctgata
     3841 ccgaacaact taacttgctt aaaaaatcac agcgagcctg gctggatttt agggacaaag
     3901 aatgtgaatt aatcctttca aatgaggacg ttcaggattt aagtgaccct tattctgaat
     3961 cagaatggct ctcatgtatg atcatacaga ccaatacgcg aactcgccag ttgcagctat
     4021 accgtaactc tgaagatttt tatccaagcc ctttgacaag aggataattc acatcttttt
     4081 ggcatgtttt gttgcaagct attcctgata aataattgca acaagacatc gagccttttt
     4141 cactgagtta ttaaacatac tcgcgagcgc gtaatttttt tgtccttcag cgataattca
     4201 caatcacttc attacgttgt acctttagcg gtggaattaa ccgcccaccg cctggcactt
     4261 cccagataaa tcgcaacggt tctgctgccg ctataccgga aaaggccacg gtggttccgc
     4321 tctgcccctc taattcgaca caacgagatt gcgaacacaa gcgaacccgc agtcctgaag
     4381 gtgtcgggcc gataagctga taacgccacg ctaccagcgt catcaatcct gaagcaggtt
     4441 gtcgcgtaga aagaggcgca gacgacatcg actcaccgcg atgatttagc gtaattccta
     4501 cactacttgc ctgccacatc ccctccccgg cggcttgcac cagcagcgga aacaataata
     4561 ttgctaataa ggttctcatt atttgccgcc aattgtcgcc gtcatgcgga tatgtcggtt
     4621 atcagacagt tccagattcg acaggaccac taactgcggc aagctgcggc gcaggaagcg
     4681 agacaataat ggtcgcagcg cgtggttcac caacaatact ggcggcgcac ccagcatctc
     4741 ctgacgggat agcgcttcct gagtttgcgc cagtaaacga tccgccagcc ctggctccag
     4801 tcctcccccg ccctgcagcg cctgtagtaa caaacgttcc agcggtgtat cgaggccaat
     4861 aacatggact tcatctttgc caggaaacca ctgctgggta atcgcccgtc ccaacgccac
     4921 gcgcacgacg gcggttaatt catgtggatc gctttggatg ggcgcatgtt ccgccagcgt
     4981 ttcgagaatg gtgcgcatat cgcgaatcgg tactttttca tcgaggagat tttgcagcac
     5041 tttatgcagt gtggtgagcg tgacgacgcc aggaacgaga tcttccgtca gctttggcat
     5101 ctcctgggcg acgcgatcca acagctgttg cgcctcctga cgaccaaaca gctctgcggc
     5161 atgctggcta atgaggtggt taagatgcgt tgctaccacc gtgctggcct caaccactgt
     5221 gtacccctga atctgcgcct gttcttttag cgcactttca atccagatag cattcaggcc
     5281 aaatgccgga tcgacggtcg cctcaccagg taacgtcccg gcagcggttc cagggttaat
     5341 cgccagccag cgccccggat aagcatcacc actgccaatc tccacgcctt tcatcaaaat
     5401 gcgatagcgg gcaggttgca gatccatatt gtcgcgaatg tgcaccactg gcggcagaaa
     5461 tcccatctcc tgggcaaatt tcttgcggat actgcgtata cggcccaaca actcaccatc
     5521 ctgctggaaa tcgaccatcg ggatcagtcg ataacccact tccattccca gagaatcttc
     5581 cagttgtaca tcgttccacg tcgcttcgac aacggtatta ttctctgcca tttttaccgg
     5641 tttgggttcg gcaggcgctt tttgttcgtg tccgcgtatc caccaggcca gcccgagcaa
     5701 tccggcagtg aacagcaaaa ataccaggtt cggcattcca ggcaccaggc cgagtaaacc
     5761 gagcacggcg gcgcttaaca acataacgct tgggttactg aaaagctgat tcaccatctg
     5821 ctcgccaaca tcctgatcgg tgctgacacg cgtaacgatg acccccgcgg cggtagaaat
     5881 caccagcgcc ggaatttgtg ccaccagacc gtcgccaatg gtcaatagcg tataactttc
     5941 cgccgcgtgt cccatgctca tgccatgttg cagcacgccg accagcaacc cgccgacaat
     6001 gttaatgacc atgatgagga tcccggcgat ggcatcgccg cgaacaaact tacttgcccc
     6061 gtccattgag ccgtaaaaat cggcttcctg agtcacttcg gagcggcgtt ttttcgcctc
     6121 atcttcacca atcaatccgg cgttaaggtc ggcgtcaatc gccatctgct tacccggcat
     6181 accatcgaga acaaagcgcg cacccacttc tgcgatacgc ccggcacctt tggtaatgac
     6241 cataaagttg atgatcacga gaatgacaaa caccacgata ccgatagcga aattgccacc
     6301 aacgaggaag tgaccgaacg cttcgaccac cttccctgcc gccgccgcgc cggtatgccc
     6361 ttccattaaa atgatacggg ttgaagccac gttaagtgcc agacgcaaca gcgtggtaaa
     6421 caacagaatg gtcggaaacg cagcaaactc aagcgtgcgc tgggtaaaca tcgccaccag
     6481 caacaccatg atcgacaagg caatattgaa ggtaaacaac aggtcgagta tgaatgcggg
     6541 cagtggcagc accatcatcg acaagatcaa caggatcaaa atcggtccgg caaggatctg
     6601 ccattgtgtc gatttcaggt ttgcgggcag gcgcagcatc gcggccagat tactcatggg
     6661 tcggtttctc gttaataaaa tccagggctt ccggcaccgg aagatgagta ggttgtacag
     6721 ggcgctgtcc accagccaga cgccagcgtt tcagttgcca gacccaggcc agcacttccg
     6781 ccaccgcggc gtacagttga cccgggattt gttgaccaat ctccgcatgt cgatacagcg
     6841 ctcgcgccag cggcggcgct tcaagcgtcg ggacgttatt ttcagcgcca atttcacgaa
     6901 tgcgcagcgc gaccagccct gcacctttag cgaccacttt cggtgcgctc attttgtttt
     6961 cgtcatactg caacgctacc gaatagtggg tcgggttatt gacaatgaca tccgctttcg
     7021 gcacatcggc catcatccga cgccgtgcgg cagctcgctg catctgacgg atccgtcctt
     7081 taacatgggg gtcgccttcg ctttgtttga actcatcacg aatatcctgc cgcgacattc
     7141 gcagcttttt caggtggctg aagatttgga aaaagacgtc aaatcccacc attggaatga
     7201 caccaagcac caccagcagt gcgcatagcc ctaccacatc catcgcatta cccatggcgg
     7261 taatcggaga ctcggccatt aagcgcatca tctgcggcca gtgatgccag agaaaaaacc
     7321 ccgtcacgct gccaaccagg atggttttca aaattgcttt aagcaactcc gcgccagtct
     7381 gagccgagaa catccgttta atgcccggta gcgggttgag tttggaaaac ttcggctgca
     7441 aggatttgcc gctaaatacc agccctccca gcatgaccgg agaaatgagc gccaccagta
     7501 ccacgccgct aatcagtggc agcagcgcca gcatggcttc tctgatcagc agaataatct
     7561 gcccgaggat cagattcggg tctttgataa tactgtgatc aaaatgcagc ccagcggaga
     7621 gcatgcccga caatcgacgg gccagcgaca caccgccaaa ccagataaca ctaacgccca
     7681 ccagcaaaat cagcagtgag gtcagttcac gggaacgcgg gatttgcccc tcttcccgcg
     7741 ctttttctag tcggtgaggt gtgggggctt ctgttttgtc gtcgctctcg tcagacacgt
     7801 cgccaatcct ggaaagagta ttaagcgtga atgatgccag agcgcaaagc gttcaatggt
     7861 ttgagtaagg ggcaaaacag gcgggattta gggcttttgc tgccacacat caagcatagt
     7921 gtgcgtttgt cggatgcggc gtcatcgcct tatcagaccg cctgatatga cgtggtcacg
     7981 ccacatcagg caatacaaat caaaatccaa gactatccaa caaatcgtcc acctgatcct
     8041 gactggctac cacaccggct ttgctggtat cgacctgagg tccattaagc aaactctggt
     8101 tttcacgttt tggacgcgac tcctgttccg ggatgttttc caacagcacc atcagcaact
     8161 ggcgttcgat ctcctgaatg acatccatca tccgcttaat gacctgcccg gttagatcct
     8221 gaaaatcctg cgccatcatg atttccagca gttgcgcgtt agtaaagctg gtatgcgcgg
     8281 gtacatctgc cagaaattgt cgtgtatctg ttaccagttc acgggcgtcg gcaaggtcaa
     8341 tcggatcggc aaaccagtca tcccaacgtt gggttaacgc ttttgctgat ttctccattt
     8401 gatcctgatg cggttgtgac gcctcaacac tgttcagcgc ccgctccgca gcctgggcgg
     8461 tcatctgcac aacatagtac aaacgatcgc gcgcatcggg gatggcttcc gccgcttcgg
     8521 caatggcctg atccagcccc agttcccgca aactgtcgcg cagcatacgc gtcaggctgc
     8581 cgatgcgcgc aatgatatcg ccagctgaat gctcgtcagc aggtttgatt gatggttgca
     8641 tcatagtcgc atcctcacat gcccagtttc tcaaagattt tgttgagttt ttcctccagc
     8701 gtcgcggcgg taaatggctt caccacatag ccactggccc ccgcttgcgc cgcagcaatg
     8761 atgttctctt tcttcgcttc tgcagtcacc attaacactg gcaatgccga catcgcgcca
     8821 tccgcacgaa ttgttttcag caattccagg ccatccatat tgggcatgtt ccagtcggag
     8881 ataacaaatc cataaccgcc tgcctgcaac ttattgagag cgtcgacgcc atcttccgct
     8941 tcctcaacat tattgaatcc cagctctttc agcaggttac gcactatgcg tcgcatggtg
     9001 gaaaagtcat ccacaaccaa aaatttaagt tctttatccg ccatttcaca ctcctgattt
     9061 aaatacgtat cgcctgtccg gcactaattt ttgccaacat ttgctggctt acctggctaa
     9121 gatcgaccac ttcgcagaca ccacccatat tgatggcctc gcgcggcatg ccgaacacca
     9181 cgcaacttgc ttcgttttgc gcaagggtcc atgcccccgc ctgacgcatc gccaacattc
     9241 ctgccgcgcc gtcgttgccc ataccggtca ggatcacccc aaccgcatta cgccccgcct
     9301 gtttggcgac agaatggaac aacacatcta ccgaaggccg atgacggtta accgccgggc
     9361 catcatgaat tttgatttgg taatttgcgc cactacgcgc cagctccata tgccgatcgc
     9421 ccggcgcgat ataggcatgc cccggcaaaa cacgttctcc gtcttcggct tctttaaccc
     9481 cgatctgaca aagcttatta agtctgtcgg caaaagagcg ggtgaaacca ggcggcatat
     9541 gctgggtaat taacagtgcc gggctggaaa gcggcaacgg ttgcagtacg tgacgaattg
     9601 cctcagttcc acccgttgaa gcaccaatcg caatcagttt ttcagaactc aacaacggcc
     9661 ctgccttcag cgttgtcggt gccgacaatg gcttatgtgc tgcaaggctc gcctttgctg
     9721 ctgtacgcac cttttcagca atcatttcgc tatacgccag cattccttcg cgaataccca
     9781 gttgcggttt ggtgacaaaa tctatcgccc ccagctccag cgcgcgcagc gtgacttctg
     9841 accctttgcc ggtcagggaa gaaaccatca ctacgggcat tggacgcaaa cgcattaatt
     9901 tttcgaggaa atccagtccg tccatccgcg gcatttcaac atccagcgtc agcacatcgg
     9961 gattgaattt cttaatcaag tcacgcgcga ccagcggatc aggcgcggtc gccaccattt
    10021 ccatgtcgct atggctgttg atgatttctg tcatgatctg gcgcatcagt gccgaatcat
    10081 cgacagataa caccctgatt ttgctcatcg ttaatcctta cttagcgcat acaccgtctg
    10141 accacgcagc gtgaagcggc gctcaaggtg gctaaagttt tcagagtgac ccgcaaacag
    10201 caatccgtcg ggtttaagga gcggaacaaa gcggcgcaaa atctcctgct gggtattttg
    10261 atcgaagtag atcatgacgt tacgacagaa gatcgcatca aacggccccg gcacggtgta
    10321 ctgtttcgcc agtagattca gcggggcaaa atcaacatag ttcgccagct cctgacgcac
    10381 gcgtaccagc ccttcatgcg gtcccgtccc tcgcatgaaa taccgttgca gttgctgcgg
    10441 cgtcaggttt ttcaactctt catggcgata aataccgctt ctggcttttt ccagcacttc
    10501 ggtgtcgata tcactggcaa acactttcca gcgtccgggc gcggtgccca atgtgtcagc
    10561 cagcgtcatc gcaatgctgt acggctcttc gccggtcgaa gccgccgcgc tccatacgcg
    10621 atactcgcca gaacggcgac gtgcgtgatc cgcgagcaga gggaaatggt gtgcctcacg
    10681 gaaaaatgcc gtcagattcg tggtcagcga attgataaac gcctgccact caccgctgtg
    10741 ctgattagat tccagcaagt tcagataatg accgaaatcc gtcagtccca gcgaacgcaa
    10801 acgacgaacc agtcggttgt aaaccatgtc gcgtttatgg tcagccagaa cgatcccggc
    10861 tcgttgatag atcaattgac ttatccgccg aaaatgcgcg tcggaaagcg ccaggcgctc
    10921 ggtcatctgt aacaataaag acgtttgccc acagggcaga gatgaagtca tagcgccttc
    10981 tcaatcactt caggatacca ctggcgcaat ttgtaactgc accgactcat gtcgtgctac
    11041 ttcatgttct tcaagggtaa ataccgccac acgcgacgaa agatggtcgg cctgattcgc
    11101 cagttgttcc gttgccaccg ccgcttcttc taccagcgag gcgttctgct gagtcacctg
    11161 atccatctgg ctgacagcct gtgcaacctg ctcaatcccc cgctgttgtt cttccgacgc
    11221 agaggcaatt tctcccataa tgtcgttcac gcgggtcacc gaactgacaa tatcgatcat
    11281 ggtcgcggcg gcgttattca ccagtttcga accctgctgg acacgattga ctgactcttc
    11341 gatcagccct ttgatctctt ttgctgcctg cgcgctacgg ctggcaagat tgcgcacttc
    11401 acctgccact accgcaaaac cacgcccctg ctctccggcg cgagccgctt ccactgccgc
    11461 attcagggcc agaatattgg tctggaaagc aattccgtcg ataacgctga taatgtcgcc
    11521 aattttttgc gagctggtgg cgatctcctg catggtgtga gtcatggtac tgacctgaac
    11581 accgcccgcc tgcgccgttg tcgcggcatt ttttgccagt tccgacgcct gtcgtgcgtt
    11641 atcggcgttt tgccctaccg tggcggttaa ttgctccata ctggcggccg tttgtgccag
    11701 cgatgccgcc tgctgttcgg tacgacttga gagatcgtta ttgcctgcga caatctccgc
    11761 gataccaatg tgcatctcct ggcttccctt acgcacatca cttaccgtcc cacgcaaagc
    11821 ctgctgcatg gtcttcagac tggcaaaaat ggcggtgatc tcattacgac catataccgc
    11881 aatcggacgc gccagattac ccgcagcaat gctgtcaaaa tggctaccga taatggccag
    11941 tggttgaaca atcatcttgc gcgtccacca cagcgcactg ctgatgtaga tcgccgcaac
    12001 aataatcatg ctgataaaca ccagtgccga aatctgatag ttacgctggc tttgcgcact
    12061 ggcggcttcc agcacatggt tgatctccag ctgccaggcc tcaaagttaa cgtcaaacgc
    12121 attctgtgat ccctgcaccg gcgcagtgag gaaatccgaa agttgattac tttcaagcca
    12181 ggtcgcctga tgttcgagat cgttgtgcca gcgggcaaaa cttttctccg tctctttttg
    12241 caatgccctg acgtgctcgt tgcccgcagt catcgccata aaacttttaa acagcgtggt
    12301 ggattgcgtc agactggcgc gcgccgtcgt catcaacgtt ttaatgtcat ccgccggata
    12361 gctaagtgcc gtcagagtgc ccgctttgtt cagcgcggta ctggcctgta acattaccgc
    12421 ccgagtttgc gctaatgccg cacgttgctg attgctctgc tcgacctgat tcagtcgttg
    12481 caaatcgtcg cgaaatgccc aaaaagacat gccgttactg ccaatctgca agatcccgca
    12541 gagaatcaaa attaaaaaca gcgtggtcga aattcgaata cgattaaaca tcaacgctcc
    12601 ccatcaggcg gcaatgaccg cgttagtaaa tactcgtcaa aatgtttccc agtttggatc
    12661 ttgttcagca attcgcagtc gtggctgtgc cggtggttgc tcactggcag gacgggatgg
    12721 tgtttgcggt ttattggtga gtgggctggc tgccagacgg aacgcggaaa ccgcttgcgt
    12781 taaacgactc gcctgttctt ccagcgcagc ggcggcggca gctgattcct gcaccagcga
    12841 tgcgttctgt tgcgtgacgc gatccatttc cgaaaccgcc aatgcgactt gatcgatgcc
    12901 acggctctgt tcatccgatg ccgatgcaat ctcgcccata atgtcagtca cgcgagtgac
    12961 agcattgacg atattgttca ttgtttcccc ggcgctttcg accagcaccg aaccggtatc
    13021 aacgcgtgag acggagtctt caatgagggc tttgatctct tttgccgcct gggcgctgcg
    13081 actggcaaga ttacgcactt cacccgccac cacggcaaaa ccacggccct gttcacccgc
    13141 acgcgcggct tcgaccgcgg cattcagcgc gaggatatta gtctggaagg caataccgtc
    13201 gataacgctg ataatgtcgg caattttctt cgaactatcg gcgatctcat gcatcgtttt
    13261 cactacgcca tccaccactt tgccgccgtg ctgggcggtg tcggaggcac tttgcgccag
    13321 ttgcgaggcc tggcgggcgt tatcggcgtt ttgcttcact gtcgcggtga gctgctccat
    13381 gctggcggca gtttcttcca gcgcggatgc ctgctgttca gtacgggagg aaagatcggt
    13441 gttgcccgcc gcaatttcac gggtaccggc atagatggca tctgaacctt cgcggacatg
    13501 agtgacggtg tcagtcaaag agcgttgcat atgtgaaacg ctctgcgcca ggtcgcccat
    13561 ttcactgcgc ccgtcaatgg tcagggtatt cgccaggtta ccaccggcga tttcgcgaat
    13621 gtgagcaata atttttgcca gcggagtaag caacatacgg cgaatgccgt accacgccac
    13681 cagcagaatc aataccacca ccagcgcgat aaccgccagt tgccactggg caaatcggta
    13741 atcatctgcg ttgtcagtga cgatatcgcg atacagtttt tcactgctga gggcgtactg
    13801 agcaaacgct tcgcccattg cattttgcat tccctgggtt ggctgagcga aataagctcc
    13861 agtattgcca taatctagat aatcaatcag ttcagttaac gctgtgtaat agtttttata
    13921 tttttcatca atattacgac tggtagcgac catttcaggt aacggtgcca tgcttttgaa
    13981 ttttttataa tgcgtcgctg cctgcgccaa tgttttcctg gcgctatcga gcaattcaac
    14041 tttggcgtta ctttgttgat tggaggaatc catcatcatc cgcaccgctg aacgactcag
    14101 gttaatgcgc gtttgcagca ttaaatccca ggttgacgtc agctcgccct gctgttcccg
    14161 caattgattg gaaaccacaa agctcttctg gctatggtga agggaagaaa aaaacagact
    14221 gccggaaata agctgtaaca gtgcgaatac ccccagcacc attaccaaca gcgtgactac
    14281 gcggatacgg ttaatcataa ggcaccttcc tgaaaacaag ttgatctcgt tatcggcaag
    14341 gaggggggaa actttattgc tgatgccacc cgccgcgaaa ttgaaataaa aaacccgatg
    14401 cgcagatcat cgggttcatt tcaattgagg aaatcgggag aattacgcca cttctgacgc
    14461 cgcgctatct aacagcgcca tctcttcgct gttcagcagt ttttcgatgt tcaccagaat
    14521 caacatccgg tcgcccagtg cgcccagtcc agtgagatat tctgttgaaa gcgtcacggc
    14581 aaattccggt gccggacgaa tttgctccgc cgtcaatgaa agcacgtctg agacgccgtc
    14641 aaccacgatg ccgaccaccc gctgtccgag attcaggacg ataactaccg tgttgtcgtt
    14701 atagtccaca tccacctggc tgaacttaat tcgtaagtca acaatcggca caataacgcc
    14761 gcgcagattc gtgacgcctt tgataaacgc tggcgtgttc gcaatccgtg ttacctgatc
    14821 gtagccacgg atctcctgca ctttcaggat atcaataccg tactcttcat caccaagggt
    14881 aaataccaga aattcctggc ctgacggctc gctggccagc tttgttacat tcgtcatacc
    14941 ggtcatattg ttaccttttt actcattcag gcggcggtgt tcgccatacg ttgttcgcgg
    15001 tttatcgcct gcaaggcgga gacatcaaca atcagtgcca cgctgccgtc gccaagaatg
    15061 gtcgcagcag aaatgccggg gactttgcga tagttacttt caaggttttt aactacaacc
    15121 tgatgttgac caattaattg atccaccagc aaggcgtagc ggcgaccgcc actttgtaag
    15181 atcaccacaa ttccttgggt agcttcggtt ttcgcgcccg cgacgttgaa cactttccac
    15241 agttcgacga tgggcagata ttcaccccgc acttccagca cccgctcgcc gccggccagt
    15301 ggatggagat cggcttcacg gggttgcagt gattccataa cagcattcag cggcagaatg
    15361 aaaacttcat ccgcaacgcg tacggacatg ccgtcgagga tggccagcgt cagcggcagt
    15421 aaaatgcgga tcgtagtgcc agtaccctgc ttcgactgga tttcaacatg accgcccatc
    15481 tcctggatat tacgtttaac gacgtccatg ccgacgccgc gcccggagac gtcggtgacc
    15541 tgctctgccg tggagaagcc tggtgcaaat atcagcattg caacttcgtc gtcgctcatg
    15601 ttttcgctga cagtcaaacc ttgcgaggcc gcttttgcca gaattcgctc acggtttagc
    15661 cccgccccat cgtcggtcac ttcaatgcaa atgttgccgc cctgatgttc ggcagacaga
    15721 attaaatttc cgacgctgtt tttacctgcg gcgagccgtt tttctggcag ttcaataccg
    15781 tgatcgaggc tattgcgtac caggtgggtc agcgggtcga taatgcgttc tatcaggctc
    15841 ttgtcgagct cagtggaact gcccaccagc gtcagttcta cctgcttgcc gagttttccc
    15901 gccagatcac gcaccagccg gggatagcga ctaaaaacat attccatcgg catcatgcga
    15961 atcgacatca ctgattcctg caaatcacgg gcgttacgtt gtaactgccc catgctggtt
    16021 atcaaatcac catgattaac cgggtccagt tcgctggaac gctgggcaag catggactgg
    16081 gtgataacca gctcgccgac gaggttaatt aattgatcaa ccttttctac cgctacacgg
    16141 atgctggtgg attcactgct gcgcgtcgtt ttttcccgct ccacgcggcc ggttggcgct
    16201 tgttcggctg ccagtttaag cactggtggg gtggatattt ttggcgagac ttctactgtt
    16261 tcaaaggtaa tctgatcggc ttcaatcaca aaacagagta ccgctgtgat gtcatcttcg
    16321 gcgatgtcgc ccggtaatat tgccgaaaga gaatccgccc ctttcaccac atcagttaac
    16381 gttgtcagat gccccagctc ttcttccagc aggtcgactt ccccggcctt caggcgcgaa
    16441 aggataattc gtcgcggcga ctgactgcga ctctgctcat cttgcggttc acttttggca
    16501 accacactta atcgggtcac tgcggatggc gtttcgcctt ttgcttctaa tgccagttga
    16561 cgcaaggcct ggcagatata atcgaagcta gcggcatccg gctcttgcga ctgtttataa
    16621 gcgtcgagct gttcttgcat gatgtccttc gtttccaaaa acagattgat aatgtcggtg
    16681 ttgagttgca tctcacctcg tctggcttca tcgagcaggt tttccatcag atgcgtggtt
    16741 tcctgcaaaa cgctgaagcc aaaagttcct gcccctcctt tgatcgagtg ggcagcccga
    16801 aagatggcat tcaattgttc ggcatctggc gcttccggct gcaaaaccag cagatgctgc
    16861 tccatatcag ccaacagttc gtccgcttca tcaaaaaatg tctgataaaa atcgcttata
    16921 tccatgctca cgctgtcacc tcggttcggc tgatggcatt gtgggaacac tgacctgtgg
    16981 tgcaacctca ggtttttcca gggcgcttac tggctcattc tggctttcgg cattttcatg
    17041 caaaatggcc tgttcggctt gtttgttcag taccagcagg ctgatgcgac ggttgacggc
    17101 atcatcaggt ccgcgatcgc ttaagcgcat cgttgccgcc atgccgacga cacgtaacac
    17161 tttgccgcta tccaaccctc cgaccatcag ttcgcggcgg gatgcattgg cccgatcggc
    17221 agaaagctcc cagttgctat atcctttctc accgctggcg taggggaaat catcggtatg
    17281 acctgaaagg ctaatacggt tgggaatacc gttcagtaca ggcgcaatgg cgcgcagaat
    17341 gtcgcgcata tagggttcga catcggcact gccggttcta aacatcgggc gattctggct
    17401 atcgatgatc tgaatacgta gaccttcctg gaccagatcg attttgagat ggggacgtaa
    17461 cgcccgcagt ttcggatcgg actctatcaa ctggtcgaga tcaccccgca atttccgcaa
    17521 tcgactttgc tccatgcgtt ttttcagctc ttcgatgttc ggctgcttat tcacttcccc
    17581 ctggctttgg gtgtaatcat caccaccgcc gggaattggg ctttcactat tagaaatgcg
    17641 atcgccgccc gtaaccgcag tcgccagtgg agtccggaag tactccgcaa tctgaatcag
    17701 ctcttttggg ctggagatgg agatcagcca catcaccaga aaaaaggcca tcatcgcagt
    17761 cataaagtcg gcataagcaa tcttccacga tccatgtgct gccccgtggc ttttggcttt
    17821 gcgtcgtttg acgacaataa tcggatgcgc ttgattcttc atgcttcctc ggttgtcgtc
    17881 tgttgttgcg gatttttcac cgcacgcaca tgctcttcca gttcaataaa cgacggacgt
    17941 tcgctggaat agagcgtttt gcgaccaaac tcaacggcga taggcggtgc gtaaccgttc
    18001 agattagaaa gcagagtgac tttgacgcac tgcatcattt tgctggtttc ggcgcttttc
    18061 tgacgtaaaa cagtcgctaa tggggaaata aatccgtaag ccaataaaat gccgaggaaa
    18121 gtccccacca tcgcatgtgc gataagcgca cccagctcgg cggcaggacg atcggctgaa
    18181 cctaacgcgt gaacgacccc cattacagcc gcgacaatac caaacgccgg aagtgagtcc
    18241 ccgaccagcg ccagactgtt cgccgggact tctgcctcgc tttcgtgcgt ctcaatctct
    18301 tcatccatca gggcttcgat ttcgaaggtg ttcatgtgac cgctgataat caggcgcaga
    18361 taatcgacga taaaatcaag catgacgcta tccgcgagga tgcgtgggta gctggcgaag
    18421 atctcgctct cacggggatt ttcaatatca cgttccagcg aaaacatccc catctgccgc
    18481 gattttgcca tcaaccgata aagcagagcc agcagatcca tatacattgc tttggtgtat
    18541 ttggagcgac gaaacagcaa cggcagcgcc ttcagcgtgc ctttaatcgc tttgccatta
    18601 ttgccgacga taaacgaccc aacccctgca ccggcaataa tcaccagttc agcgggttga
    18661 tagagtgctc caaggcttcc accggtcatc aaataaccgc cgaaaactgt accgagaaca
    18721 accaggtaac ctaataagat aagcacgaca tcatccttcc actgttgacc atgacaggat
    18781 gttcagtcgt caggcgttaa cgcgcgattg gggcaaaaaa aagcagcggt acgtcgttac
    18841 cgctgctgga atgttgcgcc tcaccgtatc agttaaacag cctgtactct ctgttcatcc
    18901 agcagttgtg ggataatatc ggcaggattc tgggaaagtt tacgtctttt tactgcccgg
    18961 gatggcggtt gacataagct gcaggcaaag ctgccaacag gctggtgagc gtgggtaata
    19021 aaattgccgc cgcagcagtt gcagctggaa agttgcagta atccactttc aacaaaccgc
    19081 accaatgtcc aggcacgagt taatgccagc agtggtcctt cttctgcttg tgggcactgt
    19141 tcaaggtata aacggtaggc tttgatcacc gcatcgacgc cattacacaa accggttttc
    19201 agtaaaaact gccatgcatt acagaacatc gaagcatgaa cgttttgttc ccaggtcata
    19261 aaccagtcgg ttgagaatgg cagcatgcct ttcggcggtg ggcttccgcg cagttcttta
    19321 taaagtttta tcaggcgtcc gcgacttaac tgtgtttcgc tttccagcat ctgcaaacga
    19381 gcgcccaggg tgatcaattc cattgccagc tgaatatccc gcgcttcctg aacaatgctt
    19441 ttttcactca tgatcaggcc cttttcttgc gcagcgcttc ttcaggctga ttaacatcat
    19501 tcagcaagcg tgttgagagc atgatgccgg tatgaatttg ctggagatcg tcaacgcggg
    19561 aatcttgcgt caactgagta atcgtctggt ggctgtcaaa acggaagtga caaaccagtt
    19621 gattggtttc tgccagctta accatttgcg gaagagtcag tgccgctaac gttgtcgcca
    19681 tttcttcatt tatgccgaga cgaaacatag cggacgcttt gtcctgaaca atcaaacgct
    19741 gtgcaagtag taaatatgac aagttgatgt cataaatgtg tttcagcaac tcggaggtat
    19801 gcattattcc cacccagaat aaccaacttt atttttatgc ggtttcaccg caccccgtga
    19861 tgtcgccggg aagccccggt aaaaaataat tagcattaga atagttgcga taagctgcaa
    19921 taagcagaac cacctttttg gtttaatatg cccttacaaa tagaaatggg tctttacact
    19981 tatctaagat ttttcctaaa tcgacgcaac tgtactcgtc actacacgca catacaacgg
    20041 aggggggctg cgattttcaa taatgtgtga tgcagatc
//
LOCUS       DAPHBJ010000074        19630 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.074, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000074 DAPHBJ010000000
VERSION     DAPHBJ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.074"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            92..634
                     /locus_tag="RHZ76_002359"
     CDS             92..634
                     /locus_tag="RHZ76_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family protein"
                     /protein_id="HDV2812991.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTETT
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            734..1936
                     /locus_tag="RHZ76_002360"
     CDS             734..1936
                     /locus_tag="RHZ76_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HDV2812992.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIANEHGWIEV
                     VESMTLSV"
     gene            2156..2938
                     /locus_tag="RHZ76_002361"
     CDS             2156..2938
                     /locus_tag="RHZ76_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDV2812993.1"
                     /translation="MLESSKVPALTRAIDILNLIACIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            2953..4158
                     /gene="rhmD"
                     /locus_tag="RHZ76_002362"
     CDS             2953..4158
                     /gene="rhmD"
                     /locus_tag="RHZ76_002362"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HDV2812994.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLSATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4215..5504
                     /locus_tag="RHZ76_002363"
     CDS             4215..5504
                     /locus_tag="RHZ76_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2812995.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            5522..6325
                     /gene="yfaU"
                     /locus_tag="RHZ76_002364"
     CDS             5522..6325
                     /gene="yfaU"
                     /locus_tag="RHZ76_002364"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HDV2812996.1"
                     /translation="MNALLSNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTA
                     EQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(6366..7268)
                     /locus_tag="RHZ76_002365"
     CDS             complement(6366..7268)
                     /locus_tag="RHZ76_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HDV2812997.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTTAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGFDRDQVLAATQLSEADLAANNH"
     gene            complement(7461..8651)
                     /gene="glpC"
                     /locus_tag="RHZ76_002366"
     CDS             complement(7461..8651)
                     /gene="glpC"
                     /locus_tag="RHZ76_002366"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HDV2812998.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(8648..9907)
                     /gene="glpB"
                     /locus_tag="RHZ76_002367"
     CDS             complement(8648..9907)
                     /gene="glpB"
                     /locus_tag="RHZ76_002367"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HDV2812999.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVHQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(9897..11525)
                     /gene="glpA"
                     /locus_tag="RHZ76_002368"
     CDS             complement(9897..11525)
                     /gene="glpA"
                     /locus_tag="RHZ76_002368"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HDV2813000.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            11798..13156
                     /gene="glpT"
                     /locus_tag="RHZ76_002369"
     CDS             11798..13156
                     /gene="glpT"
                     /locus_tag="RHZ76_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDV2813001.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13161..14237
                     /gene="glpQ"
                     /locus_tag="RHZ76_002370"
     CDS             13161..14237
                     /gene="glpQ"
                     /locus_tag="RHZ76_002370"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDV2813002.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDNLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(14279..14521)
                     /locus_tag="RHZ76_002371"
                     /pseudo
     CDS             complement(14279..14521)
                     /locus_tag="RHZ76_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            14700..15350
                     /gene="inaA"
                     /locus_tag="RHZ76_002372"
     CDS             14700..15350
                     /gene="inaA"
                     /locus_tag="RHZ76_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HDV2813003.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(15404..15658)
                     /gene="yfaE"
                     /locus_tag="RHZ76_002373"
     CDS             complement(15404..15658)
                     /gene="yfaE"
                     /locus_tag="RHZ76_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HDV2813004.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(15658..16788)
                     /gene="nrdB"
                     /locus_tag="RHZ76_002374"
     CDS             complement(15658..16788)
                     /gene="nrdB"
                     /locus_tag="RHZ76_002374"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDV2813005.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(17022..19307)
                     /gene="nrdA"
                     /locus_tag="RHZ76_002375"
     CDS             complement(17022..19307)
                     /gene="nrdA"
                     /locus_tag="RHZ76_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDV2813006.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         4532 a         4827 c         5375 g         4896 t
ORIGIN      
        1 tccttttttt cgctcattga atcctctgtg ccaggcagta caatcccgca gctttgggaa
       61 ttttgattaa ttgcttgtgg gaaaggggat aatgaaaaaa attgcgctag caggtctggc
      121 cggaatgctg ttggtttctg catcggtcaa tgcaatgagc atcagcggtc aggcgggtaa
      181 agaatacacc aatattggtg tcggttttgg gactgaaacg acgggcctgg cgttaagcgg
      241 taactggaca cataacgacg acgacggtga tgtcgctggc gtggggctgg ggttgaatct
      301 gcctctcggg ccgttaatgg cgaccgttgg cggaaaaggc gtttacacca acccgaatta
      361 cggcgatgaa ggttatgccg cagcggtagg aggtggtttg cagtggaaaa ttggcaacag
      421 cttccgtttg tttggcgagt attactactc tccggattcg ctctccagcg gtattcaaag
      481 ttatgaagaa gcgaatgctg gcgcgcgtta caccattatg cgtccagtca gtattgaggc
      541 gggttatcgc tacctgaatc tgtcgggtaa agacggtaac cgcgacaacg ccgtggctga
      601 cggcccgtat gttggggtta acgccagttt ctgatccctt ccggcgcggc attctgtcgc
      661 gccattcagt ttcttactac gccttatctc cctttgcgtt atagtgattc gataacaaaa
      721 gcaggagata accatgttaa aagtggaaat gttatccacc ggggatgaag tgttacacgg
      781 gcaaatcgtt gacactaacg ctgcctggct ggccgatttt ttctttcatc aggggttgcc
      841 attatctcgc cgcaatacgg tgggggataa ccttgatgac ttagtcacca ttcttcgcga
      901 acgtagtcag cacgccgatg tgctgatcgt taacggcggg ctgggaccga ccagcgatga
      961 tttaagcgca ctcgccgctg cgacagcaaa aggtgaaggc ctggtgctgc atgaagcctg
     1021 gctcaaagag atggaacgct atttccacga acgtggacga gtaatggcac cgagcaaccg
     1081 taaacaagcg gagctgcctg ccagtgctga atttatcaat aacccggtag gcaccgcctg
     1141 tggttttgcc gtgcagctta atcgttgcct gatgttcttt actcccggcg taccgtcaga
     1201 atttaaggtg atggtcgagc acgaaatcct gccgcgcctg cgcgagcgtt tttctttacc
     1261 gcagccgccg gtttgtctgc gtttgactac ttttggtcgt tcggaaagcg atctggcaca
     1321 aagcctggac actctacaac tgccgccggg cgtaacaatg ggctatcgct cctcaatgcc
     1381 tatcatcgaa ctgaaactca ccggaccggc aagcgagcaa caggcgatgg aaaaactgtg
     1441 gctggatgtt aaacgtgttg ccggacagag cgtgattttc gaaggcactg aaggactgcc
     1501 cgcgcagatc agtcgcgaat tgcaaaaccg ccagttcagc ctgacgttga gcgagcaatt
     1561 caccggtggt ttattggctt tgcaactttc tcgcgcaggt gctccattgc tggcgtgtga
     1621 agtggttcct tcacaggagg aaaccctggc gcaaactgcg cactggatta cagaacggcg
     1681 ggccaaccat tttgccgggc tggcactggc tgtttcgggt ttcgagaacg agcatctcaa
     1741 ctttgcgcta gccacgccag acggcacttt cgctctgcgt gtgcgtttca gcactacgcg
     1801 ctacagcctg gctatccgtc aggaagtgtg cgcaatgatg gcactgaata tgctgcgccg
     1861 ttggttaaac ggccaggata tcgccaatga gcatggctgg attgaggttg ttgagtccat
     1921 gaccttatct gtctgattcc gccactctgt gccggtttac tgcaccggca cattccccct
     1981 ccggcaaaat ttgtccaacc tgttgtactc aataagaaaa aatccgtgcg ctgattcaca
     2041 aatttgtgcg taatttctcc agtctgctgg acgaggagcg ggatttgatg cgcttcacaa
     2101 ttttactccc cctgagcaac gagactgatt ccagtatcta agactggagt ccagtatgtt
     2161 ggaatcaagc aaagttcccg ccctgacacg ggctattgat attctcaatt tgattgcctg
     2221 cattggcccc tgtagtgctg cgacgatcat tgacacattg ggaatcccta aaagcacggc
     2281 ctatttgctg cttaatgaac tcaggcgtca gcgttttctt agccttgatc accaggaaaa
     2341 tttttgtttg tggaccaggc tggtggagct ttccggtcat gcgttaagca agatggatct
     2401 ccgggaactg gctcgaccgc gtttgacgca actgatggat accaccgggc tgttatgtca
     2461 cctggggatt attgataacg gcagcgctta ttacattttg aaagtggagt catcagccac
     2521 tatcagcgtg cgttcccatg aaggaaaaag cctttcgctt tatcgctccg gaataggtaa
     2581 atgcctgctt gcctggcaac ctgcagcggt acagcaaagt attatcgaag ggctggtctg
     2641 ggagcaggcg acacccacaa cgattactca tccacagcaa cttcacgaag aactggcgcg
     2701 tattcgccgt cagggctgga gctatgacaa cggcgaagat tacgccgatg tccgttgtgt
     2761 cgctgcgccg gtgtttaacg ccaataacga gcttaccgct gcgatctcgg tggtcggtac
     2821 ccgcttacaa atcaatgaag aataccgtga ttatctggcg ggcaaggcca ttgcctgcgc
     2881 cagggatatt tcacgtctgt tgggatggaa aagtcccttt gacttacaag cctcataaca
     2941 atggagaaca tcatgaccct accaaaaatt aaacaggttc gcgcctggtt tactggcggt
     3001 gcgacagcag aaaaaggcgc tggcggcggt gattatcacg accagggggc gaatcactgg
     3061 atagacgatc atattgccac cccgatgagt aaataccgcg attacgagca gtcacgccag
     3121 tcatttggca ttaacgttct tggcacgttg gttgttgaag tcgaagcaga aaacggccag
     3181 accggattcg ccgtttcgac agccggtgaa atgggctgtt ttattgtcga aaaacatctt
     3241 aaccgtttca ttgagggtaa atgtgtcagt gatatcaaac tgatccacga tcaaatgctc
     3301 agtgccaccc tgtattactc cggctctggt ggcctggtga tgaatacgat ttcctgtgtc
     3361 gatctggctc tgtgggatct gttcggcaaa gtggtcgggc ttccggttta taaactttta
     3421 ggcggcgctg ttcgtgatga gattcagttc tacgccacag gtgcgcgtcc ggatctggca
     3481 aaagagatgg gctttatcgg tggcaaaatg ccgacgcact gggggccaca tgatggcgat
     3541 gcggggatcc gcaaagatgc cgctatggtc gcggatatgc gtgaaaaatg cggtgaggat
     3601 ttctggttaa tgctcgactg ctggatgagt caggacgtga actatgcgac caaactggcc
     3661 cacgcttgcg cgccctataa cctgaaatgg atcgaagagt gcctgccgcc acagcagtat
     3721 gaaggttatc gcgaactgaa acgcaacgcg ccagtcggaa tgatggtcac cagcggtgag
     3781 caccacggca cactgcaatc ttttcgtacg ctttcagaaa ccggtatcga cattatgcag
     3841 ccggatgttg gctggtgcgg tggtttaacc acgctggtgg aaattgccgc aatcgccaaa
     3901 tccagggggc aactggtggt gccgcacggt tcgtctgttt actctcacca tgcggtgatc
     3961 accttcacca atacgccatt cagcgaattc ctgatgacca gcccggattg ttcaacgatg
     4021 cgtccgcagt ttgacccgat tctgctcaat gagccggttc cggtgaatgg tcgtattcat
     4081 aaatcagtgc ttgataaacc cggttttggc gtcgaactca atcgtgactg caatttgaaa
     4141 cgcccctaca gccactaatc actccgggcg ttgctgtgac aacggtaacg cccacatatt
     4201 ctaaggagct ttttatgagc accgctttgc ttgacgccgt ggtgaagaaa aaccgtgtgc
     4261 gtttaattcc gtttatgttg gcgctgtatg tgctggcgtt tctcgaccgt tcgaatatcg
     4321 gttttgccaa acagacctac cagattgata ccgggttgag taatgaagct tatgcgctgg
     4381 gagcaggcat tttctttgtg gtatatgcgt ttctgggtgt tccggcgaat cttttgatgc
     4441 gcaaactggg ggccagaacc tggattggta cgacaacact gctgtgggga tttctttcgg
     4501 cagccatggc atgggccgat actgaagcga aatttctgat agttcgcact ctgcttggtg
     4561 ctgcggaggc cggatttttc cctggtatga tttatctcac ctcgcaatgg tttccgcagc
     4621 gtaatcgcgc cagcattatg gggctgttct atatgggcgc accgctggcg ttaacactgg
     4681 gatcaccgct ttccggcgcg ctgttggaga tgcatggatt tatggggcat cccggctggt
     4741 tctggatgtt tgtgattgaa ggattgttgg cagtcggtgc tggggtattc acattctttt
     4801 ggcttgatga cacaccggag caggcacgtt ttctgagtaa acaagaaaaa acgttgctta
     4861 tcaatcaact ggcaagtgaa gaacaacaga aagtgacttc tcggctgagc gatgcgctgc
     4921 gtaatggccg agtctggcaa ctagcgatta tctacctgac cattcaggtg gcggtttacg
     4981 gattaatttt cttcctgccg acccaggttg cggcattgct gggaacaaaa gtgggcttta
     5041 cagcgtcggt ggtcaccgcc attccgtggg ttgcggcctt gtttgggacc tggcttattc
     5101 cgcgctattc cgataaaacc ggtgaacggc gtaatgtcgc agcgctgaca ttactggcgg
     5161 cgggcattgg tattggtctg tccgggctgc tttctccagt aatggcgatc gtagcgctgt
     5221 gtgttgcagc tatcgggttt attgccgtgc agccagtgtt ctggacgatg ccgacacaac
     5281 tgctttccgg tacggcgctg gctgcgggaa ttggttttgt aaacctgttt ggtgcagtgg
     5341 gcgggtttat tgccccgatc ctgcgcgtga aagcagaaac gttatttgcc agcgatgcgg
     5401 cgggattact gacgctggca gcggtggcgg tcatcggttc gctgattatt ttcactctgc
     5461 gtgtaaatcg cactgttgcg cagaccgacg tggcacatca ttaaataggt taaggaacac
     5521 gatgaacgca ttattaagca atccctttaa agaacgttta cgcaagggcg aagtgcaaat
     5581 tggtctgtgg ttaagctcaa cgactgccta tatggcagaa attgccgcca cttctggtta
     5641 tgactggttg ctgattgacg gggagcacgc gccaaacacc attcaggatc tttatcatca
     5701 gctacaggcg gtagcgccct atgccagcca acccgtgatc cgtccggtgg aaggcagtaa
     5761 accgctgatt aaacaagtcc tggatattgg cgcgcaaact ctactgatcc cgatggtcga
     5821 tactgccgaa caggcacgtc aggtggtgtc tgccacgcgc tatcctccct acggtgagcg
     5881 tggtgtcggg gccagtgtgg cacgggctgc gcgctgggga cgcattgaga attacatggc
     5941 gcaagttaac gattcgcttt gtctgttggt gcaggtggaa agtaaaacgg cactggataa
     6001 cctggacgaa atcctcgacg tcgaagggat tgatggcgtg tttattggac ctgcggatct
     6061 ttctgcgtcg ttgggctacc cggataacgc cgggcacccg gaagtgcagc gaattattga
     6121 aaccagtatt cggcggatcc gtgctgcggg taaagcggct ggttttctgg ctgtggctcc
     6181 tgatatggcg cagcaatgcc tggcgtgggg agcgaacttt gtcgctgttg gcgttgacac
     6241 gatgctctac agcgatgccc tggatcaacg actggcgatg tttaaatcag gcaaaaatgg
     6301 gccacgcata aaaggtagtt attgatatca aaggcccatg gggatcggct gtgggcctgt
     6361 gttaattagt ggttattcgc tgccagatcg gcttcgctta gctgggtggc cgcgagcacc
     6421 tggtcacgat caaagccctg ttccagcatg cgcaaagcaa ttttaatggc ttgttcatgc
     6481 ataccttcct gccagccaat ttgatgccct tcctgccgta gccgttcagc agtagtcatt
     6541 aatctctcct tttgtagtgg tgaacgttcg gcaatctcct caataaaacg ggtgaaacgg
     6601 gaggtatcgc cgcattgcag cagataatta aacagtgttt gtagctggct gtcattagtg
     6661 aagcctctaa ccaaaagcgt ggtgatcctg tcgaccatgc cgattaaatc gcggtcgcga
     6721 atatgctttt gaatcagttc cagcagagct atacgccgat gttgcatgat ctcatcgtca
     6781 ggtacgatgg tgatatccac caacggaaaa gcttcggtgt acaactgccg ggcaagttgc
     6841 ggatcgtcaa actcatccag ccagttaagt gagtacgggt agggcgatgt ttcgccatga
     6901 taaaacagca atggcaccac cagcggaact ctgtcatagc ctttatccag gtgacgctgc
     6961 atggcggcag tggcatagcg cattagccga aaagccatat tcttttctgc agagctttga
     7021 tgttcaatca cgcagtagat atagccgtca ccgtcgctgg tttccacgga ccacaaaaca
     7081 tccgagtagt aagcgcgtaa acttttttca ataaaactag tgggttccag gcgtaaggtt
     7141 tgcaggttgc aaagcttgcg cagtggttct ggtaaatgta tttcgagaaa atcccgtgcg
     7201 gtttcgggtg tgaacataaa ggttttaaat accgcatcat gcggggagga ggttgttgat
     7261 tcggtcatgg ttgtccgtca cctgatattt atgatgacgc gacattacgg ttatcccagg
     7321 cctttgacat ctgccagttt cagcctttgc gagacgtttt cagaggaatt ttcactgcgt
     7381 tgcaacgcat acattgggca cggaatcgtg tacgcaaaaa tgcccggtaa ccacatgctt
     7441 accgggcatc agaaaggagt ttaagccagc gcctgggcca gtagcgtaat cggatgttcg
     7501 cagcgaagac ttgtggacat ctcaatctgc catttacagg tttcgcagtc ggtgaccacc
     7561 agatctgcgc cgctttcttc tatctggcgg aacagtggtg cgccgatggc ttgtgaggtg
     7621 gggtagttct cttttttgaa accgtaagta cccgcaatac cgcagcactg ggaatccagc
     7681 accgttaact caagccccgg gattttacgc aacagctcca gggtgtagag cgtccagccc
     7741 attttttcca tatggcacgg agtgtgataa accactttca gcggcagcgg tttcagcggt
     7801 aacgttttgc cttcgtccag cttgcgccac agccagcggg ttgccagttc gatatgatcg
     7861 cgcaagcctt tgttgtcgac attcagcact tccgggtatt cgtcgcgcag ggcaaatgta
     7921 caggttgagg aggtggcaat caccggaatg ccttttactc ccacagcttc gcggatcgac
     7981 tctacattcg taattgcctg tttgcgtgct ttatcggtaa agccgttggc gattagcggt
     8041 acgccgcagc atttttcttt gctgagcagt tgtacaccgg tacccattgc gttgagcact
     8101 ttaattaaat ctttacctaa ctgcggatgg ttgtagttaa cgaagcatcc gtgaaagaaa
     8161 gcgacctggt ctttatattg tgcttgctga gccgctatgc tgcgatacca gcgacggaac
     8221 gtgccgaagg agtatttcgg tagcgtgcga cgatgatcga tttttaacgc cgcatcaagc
     8281 agctgccgca ccggtttcag cgaggtagcg gtgttgacga ttggtgcgaa cggcgtggaa
     8341 acgctaccca tcaggtcggt atgactcaac acaaaattac gcagcgacgg gcgcgtggtg
     8401 tcatattttg cccgcgcgcg ctggataata tcgccaatct tcacatcgga cgggcaggcg
     8461 acttcacaac gtttgcagtt gatgcaatat ttcagcgcct cgtcatacag tgcgccatct
     8521 ttcaaacgca gacgctcgcc atccggcccg gcttgttttg gccctggata accgggattc
     8581 acccggctca ccgggcaggc ggtggtgcag acggtgcact taatgcagtt ttcgaagctg
     8641 gtgtcgttca ttgttggcct cctgcgcgtt gggcaatctg ttgagcggca tgtaaagcac
     8701 tgacggcaca aacaccgccg ccgcatccct gggcgatggg atcaaatccg cccagcaccg
     8761 aaccgatggc aaacaggttc tcaatggttt gccctgcctg cgacgggcgt agcgtctcat
     8821 cagtggttac accgaactgc tgccacggtt gcggcgcaaa aaaatcgccc ttataccatt
     8881 caccccgcgt ggcggtttgt agcacatcaa ggccgagaat cggctcgcga atgccgttac
     8941 gttccgctac cagtccgcca ctaaagaaac tgccgctggc gagaaccgcg aaacgtggac
     9001 gtagcggaat atcggcgtga ttgcgggtcc agatttcatt cactacgcca tttttacagg
     9061 tcactttttt cacttcatcg cccggcatcc acacgccacc ctggtgcaca aactggcgct
     9121 gtaactggtt ttgcagacga atgcccagca cggaaggcgg cagcgttggc aaaagcatca
     9181 gtgaacaagg tagtttttca ttcaaccaac gccacagttt gtcatcggcc aggccgaagc
     9241 aggcgggcat caggatcatt tcgcaggtat tggcgacagg aataagcgca tcaagtaaca
     9301 gcggccagtt ttcttcatta tcaaggaaac gggcgatatt cacagcgcga aattcggtgg
     9361 cgttattgcg caggacatcc agttccggca gctctatttc tgcggtttca accgaaagat
     9421 ccagttcgcg caacgaagcc gccgcaagat gcgcctgaaa atccatcagg ccgcttatcc
     9481 cgaccacaca tattttcttc gcgggcaacg gccagacggg gacttctggc gaacttagcc
     9541 aggtagagcg cagagtgccg agcggcgtaa tccgctggtg agccagttct acgctgccct
     9601 gcaattgcgc accgctttca gcgattaatg cctgcgcctg gcaagcgaga tcgagcacgc
     9661 gttgtggccc gagaagggag taaggatgtg caggtgcctg ctgacgcaaa gattccagtc
     9721 cactgtgaat gtctgccacg ggttgaccat ctggcagatg gctcagcaaa tccagcgaac
     9781 cggatgagaa atgcaacgcg ctttgaccac gcgtgacaat ggcacagcgc aggccgtgtt
     9841 tttgcagttg caggccacag agtaatccgg cgaggccgcc gcccataatg acagtatcaa
     9901 agcgcatctt tctgctcctt ctccagacca cacaatccct gataaaccca gcgggtaaat
     9961 tcgctttcgc gcagtgcatc tccccaggcg atgggttgca cgcctttcca gcgttcgtta
    10021 aggaaggtgg aaagttgctc gatagattgc gcggacgtcg tgacgttaaa acgttgcagc
    10081 agtccggcag cgcggcaggc gcagagttcg ccctggcagg tgcccatccc cacacgggta
    10141 cgacggcgta aatccagcag gctattaacg tttaaatttt ctaccgcgta ctgcacttca
    10201 cccgcagtta ccgcttcgca ctcacatacc aggctacggt gcagacggcc ttcgctcagc
    10261 caggcaggcg tgcgatcgcc atgacgataa accgcagaac cgcgcagcgg ggcaggcagg
    10321 gagatgactt tacgcaaggt aacttcagcg ggttcttgtg aaccaggcag tgccagatcg
    10381 gcagtcgtac aggggcgcgt gttgcccagt ttgcggcata ccgcgtcggt agcccattca
    10441 gccatcagcc gataggtcat cagtttgcca ccggtgatgg tgataaatcc gtccagacca
    10501 tcgcgttcag catggtcgag cagcacgatg ccacggctga cgttacgtcc gctcgggtcg
    10561 tcatcgctgg caaccagcgg gcgcacgcca gaataggccc gcaaaatgcg cgttttcgcc
    10621 atcacggggg ccagtttttc cccttcacgc agcagaatat caacctcttc tgccgtcact
    10681 cgattatcgt caatctcgtt gtaatcaata cgtaaagagg tggtaccaat cagcgaaatg
    10741 gtatcgccag gcaccagaat atcggcgtcg gaaggtttac ggcagcggtt gatcacatgc
    10801 tggttaatgc ggtgatccat gatcagcagc gatcctttcg ccgggaacat gcgaatgcgc
    10861 agatcggcat attcggcaat gtgttgcccc cagatcccag cggcattaac cacgacaggt
    10921 gcatgaaggg cctgagtttc gccggtgaga tggttacgta cacgaacacc gcacaccgtc
    10981 gcgccttcac gaatcagccc cgtgacttca tgagcggtaa ggataacggc accgtgttct
    11041 ttggcatcca gcatgtttgc tgcggtcaga cgaaatggat caacggtgcc atccggaact
    11101 ttcaccgcgc caatcagtgc cgggttaacg gcaggttcga taatgcgcgc ttgctgcggg
    11161 tctatagctt ctgcgctgat ccctgcttct tcgcaggcgc gaataaaagt ggcctggaag
    11221 gagaggtcat cttccggcag ggtgataaac aggccgttgg ttggttcaac gcagtgacgt
    11281 gcaatgcgtt tcaggatctg gttttcacta atgcattcgc gggccgattc cgcatcggtt
    11341 accgcatagc gcgcaccgct gtgcagcagg ccgtggttac gcccggtggc accggttgcg
    11401 atgtcgtggc gctcaaccaa aatcacgcgc agcccgcgca gggcacagtc gcgggcaatc
    11461 ccggctcccg ttgcgccgcc gccaatgata atcacgtcac ttgattgcga gtcgcgagtt
    11521 ttcattgtta tccctctgaa gttcgttttt taccatttag ccatagtaaa aacatgaatt
    11581 gtttgatttc gcgcatattc gctcataatt cgaaagtgaa acgtgatttc atgcgtcatt
    11641 ttgaacattt tgtaaatctt atttaataat gtgtgcggca attcacattt aatttatgaa
    11701 tgttttctta acatcgcggc aactcaagaa acggcaggtt ctctcactga atcaggctgt
    11761 taatcataaa taagaccacg ggccacggag gctatcaatg ttgagtattt ttaaaccagc
    11821 gccacacaaa gcgcgcttac ctgccgcgga gatcgatccg acttatcgtc gattgcgctg
    11881 gcaaattttc ctggggatat tctttggcta tgcggcttac tatttggttc gtaagaactt
    11941 tgcgcttgct atgccttatc tggttgagca gggattctca cgcggtgatt taggttttgc
    12001 cctttcgggg atctcgattg cttatggatt ttcgaaattc atcatgggtt cggtatcgga
    12061 tcgctcgaat ccgcgcgttt tcctgcccgc aggtttgatt ctggcggcgg cagtgatgtt
    12121 gtttatgggc tttgtgccat gggcgacgtc gagcattgcg gtgatgtttg tactgttgtt
    12181 cctctgcggt tggttccagg ggatggggtg gccgccgtgt ggtcgtacta tggtgcactg
    12241 gtggtcgcag aaagaacgtg gcggcattgt gtcagtgtgg aactgtgcgc acaacgtcgg
    12301 tggtggtatt ccgccgctgc tgttcctgct ggggatggcc tggttcaatg actggcatgc
    12361 ggcgctctat atgcctgctt tctgcgccat tctggtggca ttattcgcct ttgcgatgat
    12421 gcgcgatacc ccgcaatcct gtggcttgcc gccgatcgaa gagtacaaaa atgattatcc
    12481 ggacgactat aacgaaaaag cggaacagga gctgacggcg aagcaaatct tcatgcagta
    12541 cgtactgccg aacaaactgc tgtggtatat cgccatcgcc aacgtgttcg tttatctgct
    12601 gcgttacggc atcctcgact ggtcaccgac ttatctgaaa gaggttaagc atttcgcgct
    12661 ggataaatcc tcctgggcct acttccttta tgaatatgca ggtattccgg gcactctgct
    12721 gtgcggctgg atgtcggata aagtcttccg tggcaaccgt ggggcaaccg gcgttttctt
    12781 tatgacactg gtgaccatcg cgactatcgt ttactggatg aacccggcag gtaacccaac
    12841 cgtcgatatg atttgtatga ttgttatcgg cttcctgatc tacggtcctg tgatgctgat
    12901 cggtctgcat gcgctggaac tggcaccgaa aaaagcggca ggtacggcag cgggctttac
    12961 cgggctgttt ggttacctgg gcggttcggt ggcggcgagc gcgattgttg gctacaccgt
    13021 ggacttcttc ggctgggatg gcggctttat ggtaatgatt ggcggcagca ttctggcggt
    13081 tatcttgttg attgttgtga tgattggcga aaaacgtcgc catgaacaat tactgcaaaa
    13141 acgcaacgga ggctaatggc atgaaattga cgctgaaaaa ccttagcatg gcgatcatga
    13201 tgagcactat agtcatggga agcagtgcaa tggcggcgga cagcaacgaa aaaatagtca
    13261 tcgcccatcg cggtgccagt ggatatttgc cggagcatac gctgccagca aaagcgatgg
    13321 cgtatgcgca gggagcggat tatctggaac aggatttggt gatgaccaaa gacgacaatc
    13381 tggttgttct gcatgaccat tacctcgatc gtgttactga tgttgccgat cgtttcccgg
    13441 atcgggcgcg caaagacggt cgttactacg cgatagattt cacgctggat gaaattaagt
    13501 cgttgaaatt taccgaaggt ttcgatattg aaaacggtaa aaaagtgcag acttatccgg
    13561 ggcgtttccc aatgggtaag tccgacttcc gggtgcacac ctttgaagaa gagattgaat
    13621 ttgttcaggg gttaaatcac tctaccggga aaaatatcgg tatttatcca gaaatcaaag
    13681 cgccgtggtt ccatcatcag gaagggaagg atattgcggc aaaaacgctg gaagtgctga
    13741 agaaatatgg ttacaccggt aaagacgata aagtttattt gcaatgtttt gatgctgatg
    13801 agctgaagcg tattaagaat gagctggaac ccaaaatggg catggagctc aatctggtac
    13861 agctgattgc ctataccgac tggaatgaaa cgcagcagaa acagccggat ggaagctggg
    13921 ttaattacaa ctacgactgg atgtttaagc cgggtgccat gaaacaggtg gcggaatatg
    13981 cagatggtat tggtccggat taccatatgt tgattgagga gacatcgcag ccgggtaata
    14041 tcaaactcac tggcatggtg caagatgctc agcagaataa actggtagtg catccttata
    14101 ccgtgcggtc agataaactg cctgaataca ctcctgatgt gaatcagtta tatgatgctc
    14161 tgtataacaa agcgggtgta aatgggctgt ttactgattt ccctgataag gcagtaaaat
    14221 ttcttaataa agagtaatgt tactgcagga tggcgttcat gcgccatcct gtctcatatc
    14281 aaatttttcc aatcgccact aattgttcca tgcacatttg tagtaaaaac agtggactgc
    14341 cttctaccgg cttttcttta gccagcaacg aaatctgtcg atagaaagtt ggttcgagag
    14401 gaacggaaca caattcacct gcaatgcttt tcagcgttaa ctctggttgt aaggcgatgc
    14461 ctaatccctg tcgaataaaa ttcatcgctg tatccgcctg attaaattca cattttatct
    14521 gctgggcgca ctattagtgg tagtggctgg tggaatctat gcgctgcaaa tgaagggtaa
    14581 aagcggtgtc aagctagtag tggcaaaaga aattcattaa tacgacacgt ttcattaaga
    14641 ttttcctcag gtaaccacct atagtcattg tcgttaataa aaatgacagg cgatagggta
    14701 tggcagtttc agcaaagtac gacgaattta atcactggtg ggcaacagag ggcgactggg
    14761 ttgaagagcc taactatcgc cgtaatggta tgagcggggt gcaatgcgtc gagcgcaacg
    14821 gcaaaaagct gtatgtaaag cgcatgacgc atcatctgtt tcattccgta cgttatccgt
    14881 tcggccgacc aacgattgtc cgtgaggttg cggtaattaa agaactggaa cgggccggtg
    14941 tcatcgtgcc gaagatcgtt tttggtgaag cggtgaaaat tgagggtgaa tggcgagcgt
    15001 tgctggtgac tgaagatatg gcggggttca tcagcattgc tgactggtat gcccagcatg
    15061 cagtatcgcc ttattctgac gaagtacggc aagccatgtt gaaagcagtg gcgctggcgt
    15121 ttaagaaaat gcatagcatt aatcgtcagc atggctgttg ttatgttcgc catatctatg
    15181 tgaaaacaga aggtaatgca gaagctggtt ttcttgacct ggagaaaagt cgtcgccgct
    15241 tgcgtcgcga taaagcgatt aatcatgact tccgccagct ggaaaaatat ctggagccaa
    15301 tccccaaagc ggactgggag caggtgaaag cgtattacta cgccatgtaa tcgttgtagg
    15361 ccagataaga cgcataagcg ttgtatctgg cactacaacc aattcacatc tcgatttcaa
    15421 tatcgccttt tgcccggcaa caacagggca aaatttcccc cggctgaata aaggctaacg
    15481 gttcggcaat ccagtcaact tgacctgcaa ccaggcgtgt gcgacaggag ccgcagtaac
    15541 cttcgcgaca ctggtactca accgccacat tgtgggattc cagcgccgcc agaagggaag
    15601 ggtgttcatc ctggcacagc agttgtgtgc cagtgatgcg cagggtaacg cgggccatca
    15661 gagctggaag ttactcaaat cgtcggtgtc cacttccgag tcaatctgcc cgaccagata
    15721 agaactgact tccacttcct gcggagcaac ctgcacgtta tcagacacca gccaagtgtt
    15781 gatccacggg atcgggttgg agcgcgtctg gaacggcaga tccaaaccga ctgcctgcat
    15841 acggatattg gtgatgtatt caacgtactg gcagagaatg tctttattca gaccaatcat
    15901 cgaaccgtcg cggaacagat aatccgccca gtctttctcc tgttgagctg cctgaacaaa
    15961 caggtcatag cactcctgct tacactcttc ggcaatttcc gccatctcag gatcgtccgc
    16021 gccgctgcgc agcagattca gcatatgctg ggtgccggtc aggtgcaggg cttcgtcgcg
    16081 ggcaatcagg cgaataattt tggcgttgcc ttccatcaat tcgcgttctg caaatgcgaa
    16141 ggaacaagca aagctgacgt agaaacgaat cgcttccagc gcgttaacgc tcatcaggca
    16201 gagatacagt tttttcttca gctcgcgcag gctaacggtc acagttttac cgttaacggt
    16261 gtgggtacct tcgcccagca gatgccagta gctggtcatt tcgatcagct catcgtaata
    16321 gctggagatc ccttccgcac gtttctggat ctgctcgttg gtgacgatat cgtcaaacac
    16381 aacagacgga tcgttaacga tattacgaat gatatgagta taggaacggg aatgaatcgt
    16441 ttctgagaac gcccaggttt cgacccaggt ttccagttcc ggaatagaaa taagcggcaa
    16501 tagcgccacg ttcgggctac gaccctgaat ggaatccagc agcgtctgat atttcaggtt
    16561 gctgataaag atgtgttttt cgtgctccgg cagcgcctgg taatctatac ggtcgcggga
    16621 gacgtcaact tcttccggac gccagaagaa agagagctgc ttttcgatca gcttttcgaa
    16681 gatgtcatat ttttgctgat cgtagcgagc cacgttgacc ggctgaccaa agaacatcgg
    16741 ttctttgagc tgatcatttt tcgtctgtga aaaggtggta tatgccatga gtgtgtcctg
    16801 ttgggagttt aatgccggat aaggcatttt acgccgcatc ctgtgcctga tgcgacgctg
    16861 gcgcgtctta tcaggcctac aaatccgagc cgtaggccgg ataaggcgtt cacgctgcat
    16921 ccggcacccg gagcctgatg cgacgctggc gcgtcttatc aggcctacaa accgagccgt
    16981 aggccggata aggcgtttac gccgcatccg gcatctcaat atcagatctt acatgcgccg
    17041 ctttcgcagc catcgtcctg gattgacggc accagatcgt cttgtgcgtc ttcagcgccg
    17101 tcacgggtgt tctgataata cagtgttttg accccgaatt tgtaggcggt gagcaggtct
    17161 ttcagcaact gctgcatcgg cacttttcct gacgggaagc gtgacggatc gtagttggtg
    17221 ttggcagaga tcgactgatc gataaatttc tgcatgatac ccaccagttg cagataacca
    17281 tcgttacccg gcatttccca cagcagctca taggcgtcgt gcaggtgctc gtagtccggc
    17341 accacctggc gcaaaatacc gtctttcgac gctttgatgc tgacgtaacc gcgcggcggt
    17401 tcaataccgt tagtggcgtt agagatctgc gaagaagtct cggacggcat cagagcagaa
    17461 agcgtggagt tacgcagacc gtgcgttttg attgactcac gcagagcttc ccagtcgtaa
    17521 tgcagcggct cattagcgat ggtatccaga tctttcttat aggtatcgat cggcaggatc
    17581 cctttcgcgt aagtggtttc gttaaaccac gggcacgcgc cttgctcttt cgccagctca
    17641 ttagaggctt tcagcaggta atactgaatg gcttcgaagg ttttatgcgt caggttgttg
    17701 gcgctgccgt cggagtagcg tttaccgtgc ttcgccaggt agtaagcgaa gttgatcaca
    17761 ccaataccca gcgtacgacg acccatcgct ccacgtttgg cggccgggat cgggtaatcc
    17821 tgataatcca gcagcgcgtc aagtgcacga accgccagaa ttgccagctc ttccagttca
    17881 tccaggttat taattgcgcc caggttgaaa gcagacagcg tacacagcgc gatttcaccg
    17941 ttctcgtcgt tgacgtcgtt cagcggtttg gtcggcaggg ctatctccag gcacaggtta
    18001 gactgacgca ctggcgcgat ggccggatca aacgggctat gggtattgca gtggtcaacg
    18061 ttctgaatat agatacgacc ggtagacgca cgttcctgca tcatcagcga gaacagctca
    18121 acggctttca cacgctgctt gcggatgctg tcgtctttct catatttggt atacagacgt
    18181 tcaaactctt cctgatcggc gaagaacgcg tcgtacagcc ccggtacgtc ggacgggctg
    18241 aacagggtga tatcttcacc tttcagcaga cgggtataca tcagtttgtt gatttgtacc
    18301 ccgtagtcca tatgacgcac gcggttgcct tccacaccac ggttgttttt caacaccagc
    18361 aggctttcca cttccagatg ccacatcggg tagaacagcg ttgccgcacc gccgcgcaca
    18421 ccgccctgag agcaggattt caccgctgtc tggaaatgtt tgtagaacgg aatgcagccg
    18481 gtatggaacg cttcaccacc gcgaatcggg ctacccagcg cacgaatacg cccggcgttg
    18541 atgccgatcc cggcacgctg ggaaacgtat ttaacaatcg cgctggaggt ggcgttgatg
    18601 gaatccaggc tgtcaccgca ctcgatcagt acgcaggagc tgaactgacg agtcggggta
    18661 cgcacgccgg acatgattgg cgtcggcagc gaaattttaa atgtggaaac cgcgtcgtaa
    18721 aaacgcttca catattgcag gcgcgtttca cgcgggtagt tcgagaacaa gcacgcggca
    18781 actagaatat aaaggaactg ggcgctctca tagatttcgc cggtcacgcg gttctgtacc
    18841 agatatttgc cttccagctg cttaacggca gcataagaga aggtcatatc acggtcgtga
    18901 tcgataaagg tgtccatctg cttgaactct tcttccgtgt agtcttccag cagatgatta
    18961 tcgtatttgc ccatctcgac cattttcacc acgtggtcgt acagcgcagg cggctcaaac
    19021 tggccgtagg cttttttacg caggtggaag atcgccaggc gcgcggcgag atactgataa
    19081 tccggcgcat cacgggagat caggtctgcg gcagccttga taatggtttc gtggatgtca
    19141 gaggtcttga taccgtcata aaactgaatg tgggagcgca gctcgacctg ggaaatcgaa
    19201 acgttatgca gtccttctgc cgcccaatcc agaacgcgat ggattttgtc gagattgatg
    19261 cgctctgtgc taccgtcgcg ctttgtcacc agcagattct gattcatgta tgtcgtacct
    19321 gtttttggaa ttctttccgc aatagtttca tgcagactac ctgtagtacg ggaagctgct
    19381 tagaacacta tatatagggg gtatgtttgg gattcactgc aagatagtgt gaaaatgacc
    19441 ctcttgcaag tgcataactt tgtggataac tcaggaagga aaaagtggct ttcgcgcacc
    19501 ttaggtcaga caaggtgtcc gggaaagtca atgggaagag aaaaatttgt taaaaataac
    19561 tgttcggcga tttcttgaac ggcataatag gttagataaa ttgatataga tggtttatta
    19621 atctttttgc
//
LOCUS       DAPHBJ010000075        19531 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.075, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000075 DAPHBJ010000000
VERSION     DAPHBJ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.075"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..234
                     /locus_tag="RHZ76_002376"
     CDS             <1..234
                     /locus_tag="RHZ76_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813007.1"
                     /translation="SIMACNVFAFSISGGVSERSYKETEKTSAMTTTHSTKLQPSQAI
                     LFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(311..553)
                     /locus_tag="RHZ76_002377"
     CDS             complement(311..553)
                     /locus_tag="RHZ76_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HDV2813008.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            906..1826
                     /gene="lpxP"
                     /locus_tag="RHZ76_002378"
     CDS             906..1826
                     /gene="lpxP"
                     /locus_tag="RHZ76_002378"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HDV2813009.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2182..2253
                     /gene="ypdK"
                     /locus_tag="RHZ76_002379"
     CDS             2182..2253
                     /gene="ypdK"
                     /locus_tag="RHZ76_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HDV2813010.1"
                     /translation="MKYFFMGISFMVIVWASTFALMI"
     gene            complement(2318..3556)
                     /gene="alaC"
                     /locus_tag="RHZ76_002380"
     CDS             complement(2318..3556)
                     /gene="alaC"
                     /locus_tag="RHZ76_002380"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HDV2813011.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKT
                     MFRADGLLPASSKHIHENAE"
     gene            3933..5630
                     /gene="ypdA"
                     /locus_tag="RHZ76_002381"
     CDS             3933..5630
                     /gene="ypdA"
                     /locus_tag="RHZ76_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HDV2813012.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            5645..6379
                     /gene="ypdB"
                     /locus_tag="RHZ76_002382"
     CDS             5645..6379
                     /gene="ypdB"
                     /locus_tag="RHZ76_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HDV2813013.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            6392..7249
                     /locus_tag="RHZ76_002383"
     CDS             6392..7249
                     /locus_tag="RHZ76_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2813014.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(7252..9747)
                     /gene="ptsP"
                     /locus_tag="RHZ76_002384"
     CDS             complement(7252..9747)
                     /gene="ptsP"
                     /locus_tag="RHZ76_002384"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDV2813015.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNKLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGEKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQRKEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9772..10809)
                     /gene="ypdE"
                     /locus_tag="RHZ76_002385"
     CDS             complement(9772..10809)
                     /gene="ypdE"
                     /locus_tag="RHZ76_002385"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDV2813016.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWVETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(10809..11892)
                     /gene="ypdF"
                     /locus_tag="RHZ76_002386"
                     /pseudo
     CDS             complement(10809..11892)
                     /gene="ypdF"
                     /locus_tag="RHZ76_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(11907..13154)
                     /locus_tag="RHZ76_002387"
     CDS             complement(11907..13154)
                     /locus_tag="RHZ76_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDV2813017.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKTSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(13176..13502)
                     /locus_tag="RHZ76_002388"
     CDS             complement(13176..13502)
                     /locus_tag="RHZ76_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HDV2813018.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(13721..14686)
                     /gene="glk"
                     /locus_tag="RHZ76_002389"
     CDS             complement(13721..14686)
                     /gene="glk"
                     /locus_tag="RHZ76_002389"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HDV2813019.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            14890..16146
                     /locus_tag="RHZ76_002390"
     CDS             14890..16146
                     /locus_tag="RHZ76_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HDV2813020.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGALGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            16261..16587
                     /locus_tag="RHZ76_002391"
     CDS             16261..16587
                     /locus_tag="RHZ76_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDV2813021.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(16728..17966)
                     /locus_tag="RHZ76_002392"
     CDS             complement(16728..17966)
                     /locus_tag="RHZ76_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDV2813022.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            18302..19504
                     /gene="nupC"
                     /locus_tag="RHZ76_002393"
     CDS             18302..19504
                     /gene="nupC"
                     /locus_tag="RHZ76_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HDV2813023.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
BASE COUNT         4717 a         5227 c         4873 g         4714 t
ORIGIN      
        1 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
       61 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
      121 tcacaggcga ttttgtttaa gatgagagaa gatgcgccac cattaaacct cacagaagaa
      181 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
      241 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
      301 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
      361 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
      421 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
      481 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
      541 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
      601 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcatg
      661 ttaaatgcaa gactgttgtg tacggaaaaa tatttacttt gcacgattaa taatcagtag
      721 ctgaaagcag tcagcgagtg ataaagtaca gataccgcat tcatgctcct cgtggttatg
      781 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
      841 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
      901 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
      961 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
     1021 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
     1081 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
     1141 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
     1201 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
     1261 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
     1321 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
     1381 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
     1441 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
     1501 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
     1561 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
     1621 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
     1681 aaggctaccc gacagatgaa aatcaagccg ctgcctatat gaacaagatt atcgagaaag
     1741 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
     1801 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
     1861 ccataataac ctcatggttt ttatggcttt tttcttaagg ttttttcctt cgctgaatat
     1921 ctaattatta ccgtcagaaa ttaaactgtc gccgatatga gacacttgta agttactaaa
     1981 agtaaataag aaaatgcctc ttgtctcgtt tcagttttag gcgtacatta gcgccgtctc
     2041 gagtagttta gctcagaatt ctggggtgat gcccacggta aaatacaggg cttttaaggt
     2101 cgctttgccc ggtgtcaact cactgtatca ggtgtaatga agtcattcag gcgtaacagt
     2161 aattacgcgg agagatgtaa agtgaaatat ttctttatgg gcatttcttt tatggtcatc
     2221 gtttgggcca gtacttttgc cctgatgatc taaaagcaaa ctgtgcaatg aaaaacagga
     2281 gccatcaggc tcctgtttgc atttctgacg acggtgttta ttccgcgttt tcgtgaatat
     2341 gtttgctgct ggcgggtagt aaaccgtcgg cacggaacat cgttttaatc cctctgatgg
     2401 cctgacgaat acggtcgcgg ttttcaatca gtgcaaagcg aacatgcgta tcaccatagt
     2461 cgccaaagcc aatccccggc gagacacaaa ccttcgcttc gttaaggagc ttcttggcaa
     2521 attccagcga tcccatggcc gcatatggtt ccgggatttt cgcccagaca tacatcgaag
     2581 ccttcggcat ttcaaccatc cagcccgctt catgcagccc tttaaccaat acatcacggc
     2641 ggcgtttgta ctgttcggca atgtcgcgca cgcactgttg atcgccctcc agcgccgcaa
     2701 tcgccgccac ctgcaacggc gtaaaggtgc cgtaatcgtg atagctttta atacgagcca
     2761 gggcgctgac cagcgttttg ttgccaacca taaagccgat acgccagccc gccatgttgt
     2821 agcttttcga cagcgtaaag aactcgactg ccacatcgcg tgcacccggt acctgcataa
     2881 ttgacggcgc tttccagcca tcgtagacga tatcggcata ggccaggtca tggaccacca
     2941 gcacatcgta gcgtttcgcc agcgccacta ctttttcgaa gaactccagt tccacgcatt
     3001 gcgcggttgg gtttgacggg aagccgagga tcatcatctt cggtttcgga taactttcac
     3061 gaatggctcg ctccagttcg ttgaagaaat cgacaccttc caccagcggc actgagcgta
     3121 cctgcgcccc ggcaatcacc gcgccataaa tatggatcgg gtaacttgga ttcggcacca
     3181 gcaccgtgtc accatgatcc agcgtcgcca gcatcagatg cgccaggccc tctttcgaac
     3241 caatagtgac gatggcttct gattccgggt cgatttcaac gtcgtagcga tcctgatacc
     3301 agcgggaaat ggcgcgacgt aaccgcggaa tgccgcgtga agtggagtaa ccatgcgtgt
     3361 ccgggcgctg ggccacagta cataattttt cgacgatatg cggcggagtc gcaccgtccg
     3421 ggttacccat gctgaaatcg ataatatctt cgccgcgccg acgcgcagcc attttcagtt
     3481 cagcggtgat gttaaaaacg tagggcggga gacgatcaat gcgcgtaaag cgacgttcag
     3541 ggcgagtgtc agccataatt tcctcagatt aacgttagcg cccggaccgt ccgagcgacg
     3601 ctgccacaat ggtggctgtt ttgaaaatag cctgattaat ttctgtctgt ccagcgtttt
     3661 agaacatttt tatcaatggc tttaaatgag gaactcaact tcaaaaacag gaaatttttc
     3721 tacgctaaca agaaaattac caccaatttt attaacatta taatttcaat aaatttacca
     3781 tccagaagcg cattatcaag agatacattg ctaatacacc atttatcttt caatcccctt
     3841 ttaaatttat ggtttttcct caaaaacggc ctgctggtca tcagccttca ggttacctat
     3901 catagaggtt taatccttat tcagagtcac ccgtgcacga aatattcaac atgctgctgg
     3961 cggtcttcga tcgggcagcg ttaatgctta tctgcctgtt ctttctcatc cgtatccgcc
     4021 tgtttcgcga actgttgcac aagtcggcgc actccccaaa agaattgctc gccgttaccg
     4081 ccattttctc gctgttcgcc ctgttcagca cctggtccgg cgttcccgta gaaggctcgc
     4141 tggtgaacgt acgtattatc gcggtgatgt ccggcgggat tctgtttggc ccgtgggtag
     4201 gcatcattac cggcgtgatt gcgggtattc accggtattt aattgatatc ggcggcgtga
     4261 cggcgatccc ctgctttatc accagcattc tggcgggttg tatatcgggc tggatcaacc
     4321 tgaaaatccc caaagcacag cgctggcgcg tcggtattct cggcggcatg ttgtgtgaga
     4381 cgttgaccat gattctggta attgtctggg caccaactac cgcgttgggg atcgatatcg
     4441 tctctaaaat cggcattcca atgatcctcg gtagcgtctg tatcggcttt attgtgcttc
     4501 tggtgcaaag cgttgagggc gaaaaagagg ccagcgccgc gcggcaggcc aagctggcgc
     4561 tggatatcgc caacaaaacg ctaccgctgt ttcgccatgt caatagcgag tcattacgca
     4621 aggtctgcga aattatccgc gatgacattc acgccgatgc ggtggcgatt actaataccg
     4681 atcatgtgct ggcctatgtt ggcgtgggtg agcataacta tcagaatggc gatgacttca
     4741 ttagcccgac gacccgtcag gcgatgaatt acggaaaaat catcattaaa aacaatgatg
     4801 aagcccaccg tacgccggaa atccactcga tgctggtgat cccgctttgg gagaaaggcg
     4861 ttgtgaccgg aacgctgaaa atttactact gccacgcaca tcagataacc tcgtcattac
     4921 aggaaatggc ggttggtctg tcgcaaatca tctccacgca actggaggtt tcacgcgccg
     4981 agcagctacg tgaaatggca aataaggcag agcttcgcgc cctgcaaagc aaaattaatc
     5041 cccattttct gtttaacgct ttgaacgcta tttcatcgtc aatccgtctg aatccggata
     5101 ccgctcgcca gctgatcttt aatctgtcgc gttatctgcg ctataacatt gaattaaaag
     5161 acgatgagca aatcgatatc aaaaaagagc tgtatcaaat taaagactat attgccattg
     5221 agcaggcccg ctttggcgac aagctcacgg ttatctatga tattgatgaa gaggtgaatt
     5281 gctgcattcc cagcctgctg atccagccgt tggtggagaa tgccattgtc cacggtatcc
     5341 agccttgcaa aggtaaaggc gttgtcacca tcagcgttgc agagtgcggc aatcgggtac
     5401 gcattgcggt gcgggatacc gggcacggca tcgatccaaa ggtgattgag cgggtcgaag
     5461 cgaatgaaat gccgggcaat aaaattggcc tgctgaatgt tcatcatcgc gtgaagttat
     5521 tgtatggcga ggggctgcat atccgccgcc tggaaccagg aacggaaatt gcgttttaca
     5581 ttcctaacca acgcacccca gtcgcctcac aggctacgtt attgctttga gccggagtga
     5641 tattgtgaaa gtcatcattg ttgaagacga attcctggca caacaggaac tgagctggct
     5701 aattaaagag cacagccaga tggagattgt cggcaccttt gacgacggtc tggacgtgtt
     5761 gaagtttttg cagcataacc gcgtcgacgc catttttctg gatatcaata ttccgtcgct
     5821 ggatggtgtg ttgctggcgc aaaacatcag ccagttcgcc cataaaccgt ttattgtgtt
     5881 catcaccgcg tggaaagaac atgcggtaga agcgtttgaa ctggaggcgt ttgactacat
     5941 tctcaaaccg tatcaggagt cacgcattac cgggatgctg caaaaactgg aagcggcctg
     6001 gcaacaacag cagaccagca gcacgcctgc cgcgacggta acgcgtgaga atgacaccat
     6061 taatctggtg aaagatgagc gaataatcgt cacgccaatt aacgatatct attacgccga
     6121 agcgcacgag aaaatgacct ttgtctatac gcggcgtgaa tcctacgtaa tgccgatgaa
     6181 cattaccgaa ttttgcagca aactgccgcc gtcgcatttt ttccgctgcc atcgctcatt
     6241 ttgtgtcaat ctgaacaaaa tccgcgaaat cgaaccgtgg tttaataaca cctatattct
     6301 gcggctgaaa gatctggatt ttgaagtgcc agtcagccgc agcaaagtaa aagaatttcg
     6361 ccagttaatg catctttaat cgggaacttt catgaaagca ccaggcttgc ccgccgatca
     6421 acaatttttc gccgatctgt tcagcggcct ggtgcttaac ccgcaactac tggggcgcgt
     6481 ctggtttgcc agccagcctg cctcgttgcc ggtgggcagt ttatgtattg attttccccg
     6541 tctggatatc gtgctgcgcg gcgaatacgg caacctgctg gaagcaaagc agcagcgaat
     6601 ggtggaagga gaaatgctgt ttattccggc acgcgcggct aatttaccga tcaacaacaa
     6661 accggtgatg ctgttaagcc tggtgttcgc accgacctgg cttgggttat cgttttacga
     6721 tagccgcacc acgtcgttgt tgcatcctgc tcgccagatc cagcttccca gcctgcaacg
     6781 cggtgaaggt gaagcgatgc ttaccgccct cactcatctt agccgttcgc cgctggagca
     6841 gaatatcatt cagccactgg tgttaagttt gctgcatctt tgccgcaacg tggtgaatat
     6901 gccgccgggt aattcgcaac cgcgcggcga ttttctctat cacagcattt gtaactgggt
     6961 tcaggataat tatgcccagc cgctcacccg cgagagcgtg gcgcagtttt ttaatatcac
     7021 gcccaatcat ctgtcaaaac tgtttgctca gcatggaacg atgcgtttta tcgagtatgt
     7081 gcgttgggtg cgaatggcga aggcgaggat gattttgcag aaatatcatc tgtcaattca
     7141 tgaagtggca cagcgttgcg gttttccgga tagcgactat ttttgtcgcg ttttccggcg
     7201 tcagtttggt ctgacgccgg gagagtacag cgcccgtttt cagggctaac gtcagaaggt
     7261 taattctgtt tccagcagcg tcaggatact ttgtgcatct tgcgcggcaa acaacgactg
     7321 gcggaagttt ttattcacca gtttacgcgc cagctgcgag aagactttca catgattcat
     7381 cccttcgtta gcacccagcg tcagcatgat caccagttcg acttcgccca tttctgactg
     7441 ccagtcaatc ggtttcgcca gccgggcaat gctgatactg gaatgacgga tccactgaga
     7501 tttggtgtgc gggatcgcta cgccaaaacc aacgccggtg gtaacaatct ctttccgctg
     7561 ccagacatct tcttccagct caaacggatg ttcagtgcgc ccgttaacgc cgaggttgcc
     7621 gcacaggaac tggatcgcct gctctttatt gctaaaatcc tgatcaacaa agatattctc
     7681 cagcgccagc agtgggcgaa cgtcttcttc cggcgtaaag gcggtgagta acgcttcaat
     7741 ttcctgcgca ctgcggcatt cacatgcctg acgcgccagt tcccgacacg cctcgctatc
     7801 cagttgacga agctggcttt tcaccgccgg aatacgcggg ctactcatac tcagctcatc
     7861 caggcccagc ccaagcagta gcggcagata acggctttca ccgcccagtt caccgcaaat
     7921 gcctacccat ttgccccgct gatgcgcagt ggtaactatt tgctgcaaca tgcgcaggaa
     7981 cgatggcgta atcgggttat atagcggcga tacgcgcggg ttattacgat cgaccgcata
     8041 cagatactgg gtcatatcgt tggagccgat actgaagaaa tcgacctcat cgcagaagtg
     8101 gtcgatgatg tagcacaccg acggaacttc caccatgatc ccaagcgtaa tcgtctctgc
     8161 atgacgcagg ccatcgcgct taagctcaac gatcgctttt tggatctcgc ctttcaccca
     8221 taagatctga tcgaggctgt gaaccatcgg gatcatcaac tgggcgttgc cgaaactggc
     8281 ggcgcgcaaa atggcccgca gttgagtgcg gaacaggcca gcaaattccg ggtaaatacg
     8341 taccgcgcga tagccgagga acgggttctc ttcctgggga atattcagat aaggaatgct
     8401 tttatcgccg ccgatgtcca tcgtgcggaa gataatcggc ttttcgcccg ccgccagtag
     8461 cacctgctgg taggcttcaa attgctcctg ctcgtccggc gcgctgtcgc gatccatata
     8521 gagcatttcg gtacggaaca gcccgacacc ttccgcgccg ttggcaaacg cgcctggcgc
     8581 ttccagagcg gtgccgatat tcgccgcaat atcaatacgc ttgttatcac gggaataggc
     8641 cagctgcgcg gcagcctgcg cctgttgttt ttggcgttta tccgccagcg tctgcgcgac
     8701 ctgataataa ccgctcaccg cgtcattcgg gttaatcgcc agcacgccgc actgggcgtc
     8761 aagcactgca ggttgcccgg cataacgggc aatcgcatcc agcggcaagc cactcagtac
     8821 cgggatcgcc gaggcacggg ccagaatcag tgtatgcgag gtgcgcccgg ttttctccag
     8881 aatcatgccc gcaagatttt tcaaatcaag gctcaaaaac tggcttgggg ttaaatcttc
     8941 agccaccaga atggtcggtt tttcaagcac cagcttgttg cgcggcttca gttccggcca
     9001 ggtgatatgc agcaactgtt cgctgatatc gcgaatgtcg ctaacacgtt cgcgcagata
     9061 atcgctggca gaggcagaaa gtttggcgca aacctgctcc atattgctga tgatcgccgc
     9121 ccccagcccc tgatgctgtt ctgtcatcag gcgacggata ttgcctgcaa attcatcatc
     9181 ctgaatcagc gacaaatggg cgctgaggat agttttgctt tcgccgtcac gctcacgcaa
     9241 ttgctggttc agttgctcgg caagcgttgc caggctgtgc tccagccggg tggaatcttg
     9301 cgcactggcg gggattgccc gataactgtc gaggctgtcg ctttgtaaca gggtcagcgt
     9361 acccacgccg acgccgcttg ccagcacatt gccgtacagt aaatccgggt taaggcggct
     9421 taatgaacgc ggcagcggat gcgccgtcag ttctgcctgc gtaggctgaa cgctgtcgct
     9481 gtcgataaag cgcacctgga tgtactcttc cagcacgcgc cgcgcctgct cttcatcgct
     9541 gccgctaatg ttcaggctgc aactgtcatt aaatagggtg ccggtgccaa tcagcgccag
     9601 cgagcttttc gcatctgcct ttgcgttctg gcgatggtta ataaaagtga tttcgctttg
     9661 ccactggctg cactgttctt taagttccca cgccggacga gcatgtagac cgttaggcaa
     9721 aggacagaga aattgaatcg ttaacataac cgctccgggt taatcaggag atcatctgaa
     9781 atccgtcagt tgaacaaccg tctcacgcgt aagacgttga ataagggcag ataaaagttg
     9841 ctgcatctgc aaaatgtcgc ggcaatcggc aatcgatgcg gcgcaatgtc catggcgggt
     9901 tgctggcccc atcaccactg tgggcacgcc ggtgccggtt aagtgtaccg ccccgccgtc
     9961 cgtgccgccg ttgctgaaca tatctgcctg caacggcacg ccaatttctg ccgccacggt
    10021 ttcgacccag gcggtaagtt ttggcggcgc aatcagcgac ttgtcgctta acaccagcat
    10081 cggcccgtta ccaatctggc gatggttagc cgcgccataa tcaaagtttt tcgcccagca
    10141 ggcggtatca agcacaatgg cgacgtccgg cgacaccgcg cgggtggcag tttgcccgcc
    10201 gcgtaatccc acctcttcgc tggaacttgc caccagccac acttccgcag gtagttcggc
    10261 gtcgtgcagt tcgcgcagta acgtcaccag cagatagcaa ccgaggcggt catcaaaggc
    10321 tttccccatc actcgctggt gagggagaac ctgaaaagtg gtatcaaacg tgacgcgatc
    10381 gccgggacga attcccgcct gcatcacttc gtcataggag cgcgcaccaa tgtccacgcg
    10441 catggcgctg acgtcattcc cctgccggtc gccgtcaagc aggcctggaa ttttgcactc
    10501 ttcacgggtg gtgatgcgca ccggctgcag ctggcgggca gccatgcgta cgttgccaac
    10561 cggcagcaca tcaatcgccc cttcgcggga gatgctgcgc accataaatc ccacttcgtc
    10621 catatgcgca cagatcatca ccttcggacc tgtcgattca ttcaggcgga tcagcaccga
    10681 tcccagacca tcaaatcgca cttctttttg caggcgatcc gcttcttcca gcaggatctg
    10741 ccgcacttcc tgttccgagg aggcgatcgc atctgcctcg ctcaacgctt ttaatagcga
    10801 taaatccatt atgcctctcc cgtgagcaac actgttttcg gcatggcgta gagcacttct
    10861 gcgccttgcg gggtaaccag cacaacatct tcgatgcgca cgcccccttg ccctagcaaa
    10921 taaatccccg gctccacggt cagtaacatg cctggctgta gcgtcgtggt gtcccgcggt
    10981 gaaaaacgcg gatcttcatg aacttcaatg ccgatagcgt gaccggtgtt atgaccgaaa
    11041 tagtcgccat aacctgcttc tgtaatgacc cggcgcggca tcgtcaacct gctggcagcg
    11101 cacgccgggg cgaatcgcgg agattgctgc gagctgtgcc tgcaggacaa tttgatagac
    11161 gttaaacagc gggtgagatt cggcgctcac cccttcgcca ttcaccagca aggtgcgcgt
    11221 catatcagag cagtagccct gatacagcgc accgaaatcg agagtgacaa actcgcccgc
    11281 tgcaacaatc ttgtcgctgg ctttgccgtg cggcagcgcc ccacgccagc cactggcgac
    11341 aatggtgtca aaagaggctt tttctgcgcc ctgctggcgc ataaaccact ccagttcagc
    11401 ggctatctcg cgctcgctca tccccgcctg aataaagcgg cgaatatgct ctgcaccgcg
    11461 atcagcaatc ccacaggcga ggcggatttt ctccacctcc tctggcgttt tgatttgccg
    11521 cagcacatcc ggcgtggcgc taaccagttt cgcattgagt tcagactgct agcggtgcgc
    11581 ggtttcccaa ctcacctgct ggccctcaaa accgagcgtt tgcaactgtt catcggcaat
    11641 gatttgattg acgatagtgg taagcgtgtt cgtcgcgtca agcaaatgca gctggtagcc
    11701 ttgcgcacgg gcttccacct caacgtaata gcgcgaatcc accagaatgt gcgcactttc
    11761 acggctaatc accacataac ctgatccggt ggagatcccc agatgcggct gtttgttctg
    11821 ccgtgaggag agaagcactg catccagttg ttgcgcctta agccagtcgc gcagcgaagc
    11881 gagtaatgtc atctggattt tccttcttac aggctatcga ttaacaattt gcctttacgg
    11941 aacatcatca gacgcaggaa caccaccatc agcgcagtta tgactgcccc cagcgcgatc
    12001 cccgccatat agacgcccag gttagtgacc agcggccatg cccagatagc ggactccggg
    12061 aaccattgca ctgcgcccag ccagacagcg gcggttgagc cgacaatagc gccgaccata
    12121 tacgacggaa tggcggtgat ggggctttcc agcgcaaacg gaatcgcacc ctcactgatc
    12181 cccataaacg ccaggaacat ggcggtctta ccctgcggat aaagttgagc gttgaacagg
    12241 cgtttgccgg ttaaacgacg gtcgataatg gtcgccagcc ccagaccaat cggcggaata
    12301 acgatagcga tagaacgagc ggtgaccggt aatacgtggt cagtggtaaa gctgaaggca
    12361 acaaaacctg cggctttgtt gatcgggcca ccaaggtcga ttgctgtcgc cgcggcgatc
    12421 cccattgcgt acatcagcgc gcccttctca cctgcggcag tcagcacagt acggataccg
    12481 ccgttgatcc agccaccaaa gggggtgatg acgtagtaca tcgccagcat gacaaaaatg
    12541 gcggaaagaa tcgggagcaa aaatgtggtt ttgaacgcta acaggaaatc cggtaactgg
    12601 attttctggt tcatccactt cacaaggtaa cccgcgacga tagaaataat cagcgcacca
    12661 atgaaggtgg acggcaccgg cgaagaggtc gcccattgca tagtgctggg atcgaagttc
    12721 agcagttgcg tcggctgggt agacatcaac ccgccgataa aaccagccgg gaacgccagt
    12781 ttgccaccga tagagttcgc cacaaaagca gcaaacatcg gaatggcaaa accaaacaac
    12841 acgccgccga aggactgcga cagccaggca aatttaagta aggagaggtc gaaaccggag
    12901 aatttcccgc tattaagagc atccatgatg ccgatctcag caggaatttt aagccagcta
    12961 taagcaatca gctggctgaa agcgaggatc acaccgccca taattaaggt cggcaccatg
    13021 cgtgaaatcc ctgacatcac atgctgcggg agttcacccc agaagctggt tttagaggcc
    13081 tgcggattct taactgccgc tgccgcaccg gatgcgccag gcacaacggt tgcactgcgt
    13141 tttttaatgg ccataacaat tatccctgtc gaatattact gttgttcaga ggcaatcatc
    13201 tcttcgattt ctttgatgat gcccgcagcg tttttaattg cgtcctgcaa agtgatttca
    13261 taaacgtcgc gtgattcgaa acgttcgtta tcttccgggg taactgccac ggagtggatg
    13321 atgatggtcg cttcggcgat atcctgcgcc gtcaggcgat tctggatacc gtccgcgccc
    13381 tgggtttcaa ttttcacttc ataaccggct tctaccgccg cttcttccag cgcctgagcg
    13441 gccataaagg tgtgagccag gcccatcggg caggcacata aggcaatcag tttcttactc
    13501 atcgttaatt cctcgttcag tgatagttga cgagtgaaat tatgaacgtg caaaacaaat
    13561 cgccgttacc agacaaataa tgcatttact ggagaattaa tcccgctgta cggaagtgtg
    13621 acgcaggtcg gccttgtgag gggacacaaa gaaaaattga ataaactgta tgatttaaaa
    13681 gattatcggg agagttacct cccgatataa aaggaaggat ttacagaatg tgacctaagg
    13741 tctggcgtaa atgtgcaccg gaaccgagaa ggcccggatt gtcatggacg atgagataca
    13801 ccggaatatc atggacatat tctttaaagc gccctttatc ttcaaatgcg gcacggaaac
    13861 cggaggcttt gaagaactca aggaagcgcg gcacgatacc gcccgcaata aacacgccgc
    13921 caaatgtccc gagattgagc gccagattgc cgccaaaacg gcccataatg acgcaaaaca
    13981 gcgacaatgc gcggcggcaa tcggtgcagc tgtcagccag cgcgcgttcg gtaatatctt
    14041 ttggcttgag attttctggc aggcggttgt cagctttcac aattgcgcga tacaaattca
    14101 ccagcccagg gccagaaagc acgcgctccg ccgaaacatg accaatttcc gcacgcaata
    14161 tttcgaggat aatggcctct tcttcactat tcggcgcaaa atcaacgtga ccgccttcgc
    14221 ctggcaagct tacccaacgc ttatcgacat ggaccagatg cgcaacccca agccccgttc
    14281 cggcaccgta aaccgcaata ggcttacctt cgaccggttc tgcgccacca aactgaatca
    14341 gatgctcttt tttcagcatc gggatcgcca tcgatacagc ggtaaaatcg ttaataattt
    14401 ccagatggct aaaaccgaga ttctttttca tttcggcaat tgagaacgcc caggtatggt
    14461 tggtcatcgc cacccagtca ccggtaattg ggcaagcgat ggcaatacag ccgtctttca
    14521 cctcgacctt atgttcttca agataaacgc gaatgaccgc ttcgaggctg gggtaatcaa
    14581 gccctgaata ggtcttagcc tgcgagattt caccactggc aatatcacac agagcaagac
    14641 gtgcgttggt gccgcccaca tcaccgacta atgcatactt tgtcattctt caactgctcc
    14701 gctaaagtca aaataattct ttctcacact gtaaatacct gggggcataa caacaacgcc
    14761 ggaaaggcag gctccctgta aatatcgatc tgggtcacac aattacttta tcgtttcagc
    14821 accaattgca gcgatgcttt tttgcaagct gggcaaacta agtatctgac cccgcataag
    14881 gaatagaaca tgctccatcc gcgagccaga accatgttgt tattatcgct ccccgccgtg
    14941 gcaattggga ttgcgtccag tcttattctg attgtggtga tgaaaatcgc ctcggtatta
    15001 cagaatttgc tctggcaacg actgccggga gctctgggga tagcccagga ttcacccctc
    15061 tggatcatcg gtgtattaac gctaacgggt attgcggtgg ggttggttat ccgtttcagc
    15121 cagggtcatg ccggaccaga ccccgcctgt gaaccgctga tcggcgcacc ggttccgccc
    15181 tctgcgctac ctggacttat cgtagcatta attctcggtc ttgctggcgg cgtcagcctg
    15241 gggccggaac atccgatcat gaccgtcaat atcgcccttg cggttgccat tggcgctcgt
    15301 ctgttaccgc gcgtcaaccg aatggagtgg actattttag cctctgccgg aaccatcggc
    15361 gcactgtttg gcactcctgt tgcggcggcg ttgatatttt cgcaaacctt aaatggcagt
    15421 agtgaagttc cgctatggga tcgtctcttt gcgccgttaa tggcggcagc agctggtgca
    15481 cttactaccg gattattttt ccatcctcat ttttcactgc ccattgctca ttacggacag
    15541 atggaaatga ccgatattct cagcggtgca attgtcgcgg cgattgccat cgcagcaggg
    15601 atggtcgccg tatggtgctt accacggttg cacgcgatga tgcatcaaat gaaaaatccg
    15661 gtgctcgtgc tgggtattgg cggatttatt ctcggtattc tgggggttat tggtggacca
    15721 gtttcgctgt ttaaagggct ggatgagatg cagcagatgg tggcaaatca ggctttcagc
    15781 accagcgatt actttttgct ggcggtaatt aaacttgccg ccctggtcgt tgctgccgcc
    15841 agtggctttc gcggtgggcg aatcttcccg gcagtgtttg tcggcgtggc attagggttg
    15901 atgctgcatg agcacgttcc cgccgtacca gcggcaataa ccgtttcttg cgctattctc
    15961 ggcatcgtgc tggtggtaac acgcgatggc tggttaagtc tttttatggc ggcagtcgtt
    16021 gtacccaata ccacattgct accgctgctc tgtatcgtca tgcttccggc atggctgtta
    16081 ttagcaggta agccgatgat gatggtcaat cgtccgaagc aacagccacc ccacgataac
    16141 gtttagcaaa aaatgctctc ctttatgatt taagagttat ggcgatgata cggggcttta
    16201 cctccccgta atattgcgtt aacaggccgc tgacggcttc gagcgtgaag gagaataaca
    16261 atgttcaggt cactgtttct ggcggccgcc ctgatggcat ttaccccgct tgcagcaaac
    16321 gcaggtgaaa tcaccctact gccatcaatc aaattacaaa ttggcgatcg cgatcattac
    16381 ggtaattact gggacggtgg tcactggcgc gaccgtgact actggcatcg caattatgag
    16441 tggcgcaaaa accgttggtg gcgtcatgat aatggctacc accgtggctg ggataagcgt
    16501 aaagcgtatg agcgtggcta tcgtgaaggc tggcgcgatc gtgacgatca tcgcggaaaa
    16561 ggccggggtc atgggcaccg ccattaaacg cgtccgccaa tggagcacaa tgcctgatgc
    16621 gacgctagtg cgtcttatca ggcctacaaa ccggctcttt aacgcgtagg gcggataagg
    16681 cgtttacgcc gcatccgcca atagtgccag atgcgacgct cattcaacta caatcccagc
    16741 gccgtcccca ccaacaacca gatattcagc gccacgacca gcaccacaat cacccagcct
    16801 gtctgtttta cgcgtttgct gttcaccaga tcgcccatca acttgctgtc actggtgaaa
    16861 atcagcagtg gaaccagcgc cagagcgata ccaaaactta acagtacctg actcataacc
    16921 agaatccgtg tcggatctaa tcccatcaga atgacaataa atgacggcaa catggtgact
    16981 gtacgacgca cccacagcgg gatatgaaag cgaatgaagc cctgcatcac cacctgcccc
    17041 gccagtgtcc ccaccaccgt tgaagacagc cccgcagcaa ccaggcttaa tccaaagacc
    17101 gttgccgcag cgtggcttaa cagcggttgc agcgtcagat aagcctcatc aagatcggca
    17161 acaccagtat gaccggaaaa gtggaacgcc gccgcagctg tagccatcat cgccagattg
    17221 acaaaaccgg caatagtcat ggcgatagcc acatcccatt tggtggcgga ataacgttgt
    17281 tgacgcgaac cgccatgtaa atgctgagtg agcgaggagt gcaaataaat cacatgcggc
    17341 ataatcgtcg cccctaacac gcctgctgcc aggaagaccg cttccgaagt aggtaaactc
    17401 gggatcacca ttcctttacc cagctgcgcc aggttaggct gggagaaaat caactcgaca
    17461 atgtaagccg cggcaacaaa caacagtaac ccgccaatca ctttctccag cggtttttgc
    17521 ccgcgacgtt gcagcattaa aatcaggaaa gtcgcgatcc ccgtcagcac cgcgccctgc
    17581 aacaacgaaa caccaagaat gagtttaaaa ccgatcgccg caccaataaa ttccgccagg
    17641 tcggttgcca tcgcaataat ttctgcctga acccaataga accacactac gggacgcgga
    17701 tagtgatcgc gaatctgctc cgccagattt ttaccggtgg caatccctag tttggcagag
    17761 aggatctgaa tcagcatcgc catcaggttg gcccaaacga caacccacag tagctgatag
    17821 ccgaagctag cacccgcctg aatattggtc gcaaagttac cgggatcgat ataaccaatc
    17881 gccgcaatga acgcaggtcc cattaatgcg agcctcatct tgcgcgccgc ccgtccgctg
    17941 ctactctcaa cgcgatagtt cgtcatcttg tgcctctaaa acatagcctt tgctatgttt
    18001 catgctatgc caaacgagaa tgattatcaa attcatttaa atggattgtg gtgatttctc
    18061 tgatagacca ggattatgac tacgaaaaga ttgatgactt gaatgttatg gttatgttta
    18121 atgttagcac atttacataa ctttcagctt ccatacacaa catagcagaa atgtatgaca
    18181 gatcactatt tttgaagcct gtcacaggac gtcattatag tgtgtgtcag atctcgtttt
    18241 ccttaaccat gttacataga atgtgcacgg aaatttaacc tgcctcatat ttggagcaaa
    18301 tatggaccgc gtccttcatt ttgtactggc acttgccgtt gttgcgattc tcgcactgct
    18361 ggtaagcagc gaccgcaaaa aaattcgtat ccgttatgtt attcaactgc ttgttatcga
    18421 agtgttactg gcgtggttct tcctgaactc cgacgttggt ttaggcttcg tgaaaggctt
    18481 ctccgaaatg ttcgaaaaac tgctcggatt tgccaacgaa gggactaact tcgtctttgg
    18541 tagcatgaat gatcaaggcc tggcattctt cttcctgaaa gtgctgtgcc caatcgtctt
    18601 tatctctgca ctgatcggta ttctccagca cattcgcgtg ttgccggtga tcatccgcgc
    18661 aattggtttc ctgctctcca aagtcaacgg catgggcaaa ctggaatcct ttaacgccgt
    18721 cagctccctg attctgggtc agtctgaaaa ctttattgcc tataaagata tcctcggcaa
    18781 aatctcccgt aatcgtatgt acaccatggc tgccacggca atgtccaccg tgtcgatgtc
    18841 catcgttggt gcatacatga ccatgctgga accgaaatac gtcgttgctg cgctggtact
    18901 gaacatgttc agcaccttta tcgtgctgtc gctgatcaat ccttaccgtg ttgatgccag
    18961 tgaagaaaac atccagatgt ccaacctgca cgaaggtcag agcttcttcg aaatgctggg
    19021 tgaatacatt ctggcaggtt tcaaagttgc cattatcgtt gccgcgatgc tgattggctt
    19081 tatcgccctg atcgccgcgc tgaacgcact gtttgccacc gttactggct ggtttggcta
    19141 cagcatctcc ttccagggca tcctgggcta catcttctat ccgattgcat gggtgatggg
    19201 tgttccttcc agtgaagcac tgcaagtggg cagtatcatg gcgaccaaac tggtttccaa
    19261 cgagttcgtt gcgatgatgg atctgcagaa aattgcttcc acgctctctc cgcgtgctga
    19321 aggcatcatc tctgtgttcc tggtttcctt cgctaacttc tcttcaatcg ggattatcgc
    19381 aggtgcagtt aaaggcctga atgaagagca aggtaacgtg gtttctcgct tcggtctgaa
    19441 gctggtttac ggctctaccc tggtgagtgt gctgtctgcg tcaatcgcag cactggtgct
    19501 gtaagaccat acataaaaaa gccggggata a
//
LOCUS       DAPHBJ010000076        19519 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.076, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000076 DAPHBJ010000000
VERSION     DAPHBJ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.076"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..573
                     /locus_tag="RHZ76_002394"
     CDS             <1..573
                     /locus_tag="RHZ76_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDV2813024.1"
                     /translation="GASLARLAQKGIYIAAVVMTTGNSGTDGIIDRHEESRNALKILG
                     CHQTIHLNFADTRAHLQLNDMISALEDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVY
                     QASMVACRTIPQILGYETPSTWLSFMPQVFESVKEEYFTVKLAALKKHKSQERRDYMR
                     HDRLRAVAQFRGQQVNSDLGEGFVIHKMIL"
     gene            583..1779
                     /locus_tag="RHZ76_002395"
     CDS             583..1779
                     /locus_tag="RHZ76_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDV2813025.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            1757..2338
                     /locus_tag="RHZ76_002396"
     CDS             1757..2338
                     /locus_tag="RHZ76_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDV2813026.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRIYHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNSVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2340..3113
                     /locus_tag="RHZ76_002397"
     CDS             2340..3113
                     /locus_tag="RHZ76_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HDV2813027.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDVWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3300..3575
                     /gene="frmR"
                     /locus_tag="RHZ76_002398"
     CDS             3300..3575
                     /gene="frmR"
                     /locus_tag="RHZ76_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HDV2813028.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3610..4719
                     /gene="frmA"
                     /locus_tag="RHZ76_002399"
     CDS             3610..4719
                     /gene="frmA"
                     /locus_tag="RHZ76_002399"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HDV2813029.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            4811..5644
                     /gene="fghA"
                     /locus_tag="RHZ76_002400"
     CDS             4811..5644
                     /gene="fghA"
                     /locus_tag="RHZ76_002400"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDV2813030.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVANADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKEAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(5770..6309)
                     /locus_tag="RHZ76_002401"
     CDS             complement(5770..6309)
                     /locus_tag="RHZ76_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HDV2813031.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6411..7622)
                     /gene="mhpT"
                     /locus_tag="RHZ76_002402"
     CDS             complement(6411..7622)
                     /gene="mhpT"
                     /locus_tag="RHZ76_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HDV2813032.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVIAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(7897..8910)
                     /gene="dmpG"
                     /locus_tag="RHZ76_002403"
     CDS             complement(7897..8910)
                     /gene="dmpG"
                     /locus_tag="RHZ76_002403"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HDV2813033.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(8907..9857)
                     /gene="mhpF"
                     /locus_tag="RHZ76_002404"
     CDS             complement(8907..9857)
                     /gene="mhpF"
                     /locus_tag="RHZ76_002404"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HDV2813034.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(9854..10663)
                     /gene="mhpD"
                     /locus_tag="RHZ76_002405"
     CDS             complement(9854..10663)
                     /gene="mhpD"
                     /locus_tag="RHZ76_002405"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HDV2813035.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQHDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10673..11539)
                     /gene="mhpC"
                     /locus_tag="RHZ76_002406"
     CDS             complement(10673..11539)
                     /gene="mhpC"
                     /locus_tag="RHZ76_002406"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HDV2813036.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11557..12501)
                     /gene="mhpB"
                     /locus_tag="RHZ76_002407"
     CDS             complement(11557..12501)
                     /gene="mhpB"
                     /locus_tag="RHZ76_002407"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HDV2813037.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12503..14167)
                     /locus_tag="RHZ76_002408"
     CDS             complement(12503..14167)
                     /locus_tag="RHZ76_002408"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HDV2813038.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFARELEAFSQQNDEVTLHLKTAEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            14346..15191
                     /gene="mhpR"
                     /locus_tag="RHZ76_002409"
     CDS             14346..15191
                     /gene="mhpR"
                     /locus_tag="RHZ76_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HDV2813039.1"
                     /translation="MSINMQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAEL
                     SGLHRTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLG
                     DLLREVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFC
                     PEQERKELIEMLASRPGDDYQLAREPLKLQAILARARKEGYGQNYRGWDQEEKIASIA
                     VPLRSEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRS
                     GVHLR"
     gene            15268..16350
                     /gene="lacI"
                     /locus_tag="RHZ76_002410"
     CDS             15268..16350
                     /gene="lacI"
                     /locus_tag="RHZ76_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HDV2813040.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16473..>19519
                     /gene="lacZ"
                     /locus_tag="RHZ76_002411"
     CDS             16473..>19519
                     /gene="lacZ"
                     /locus_tag="RHZ76_002411"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDV2813041.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPH
                     LTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRY"
BASE COUNT         4574 a         5368 c         5108 g         4469 t
ORIGIN      
        1 ggcgcatcgc ttgctcgtct tgcgcaaaaa ggaatttata tcgcagccgt ggtgatgact
       61 accggcaact ctggtacaga tggaataatc gatcgccatg aagaatcgcg caacgcccta
      121 aagatattag ggtgccacca aactattcat cttaattttg ctgacacccg cgctcattta
      181 cagctcaatg atatgatttc cgctctggaa gacatcatta aaaatcaaat tccttctgat
      241 gttgaaatca tgcgggtata taccatgcat gatgccgacc gccatcagga tcatctcgct
      301 gtttatcaag cttcaatggt tgcctgccgc actattccac aaattctcgg ctacgaaacc
      361 ccaagtacct ggctttcatt tatgcctcag gtttttgaat ccgttaaaga agaatatttc
      421 acagtcaaac ttgctgcatt aaaaaaacat aaaagccagg aacgacgcga ttatatgcgt
      481 catgatcgcc tgcgtgcagt tgcacaattt cgcgggcaac aggtcaatag cgatctgggt
      541 gaaggctttg taattcataa aatgattctt tgagcaaatt gcatgaaaac ctggatattt
      601 atctgtatgt ccatagcaat gttgctatgg tttttaagta cgctaagacg taaacccagt
      661 caaaagaaag gctgtattga cgccattata cctgcgtata acgaaggccc gtgtctggcg
      721 cagtcactgg ataatctact gcgtaaccct tatttttgcc gggtaatttg cgttaacgac
      781 ggctccacgg acaataccga agcggtcatg gcggaagtca aacgcaaatg gggcgaccgc
      841 tttgttgccg tcacgcaaaa aaataccggt aaaggtggtg cgctgatgaa tggcctcaat
      901 tatgccacct gcgaccaggt ttttttaagt gatgccgaca cctatgttcc gcccgatcaa
      961 gacggaatgg gctatatgct ggcagaaatt gagcgcggtg ctgatgccgt aggcggcatt
     1021 ccctctactg cgttgaaagg cgcggggctg ttaccgcaca tccgcgcgac cgtaaagttg
     1081 ccgatgattg ttatgaagcg cacgctacag cagctcctgg gcggcgcacc gtttattatc
     1141 agcggtgcct gcggaatgtt ccgtactgat gtattgcgta agttcggttt ctcagatcgt
     1201 actaaagtcg aagaccttga tctcacctgg acattagtgg caaacggcta ccgtattcgg
     1261 caggcgaatc gctgcatcgt atacccacag gaatgcaaca gtccgcgtga ggagtggcgt
     1321 cgctggcggc gttggattgt gggctacgcg gtctgtatgc gcctgcataa aaggctttta
     1381 tttagccgct tcggtatctt cagtatattt cctatgctgt tggttgtgat ttatggcgtt
     1441 gggatttatc tcactacctg gtttaatgaa ttcatcacca ccgggccgca tggtgtggtg
     1501 ttggcaatgt ttccgcttat ttgggtcggc gtagtttgtg ttattggtgc ttttagcgcc
     1561 tggtttcatc gttgctggtt gttggtgcct ttagcgccgc tttccgttgt gtatgtatta
     1621 ttagcttatg ccatctggat tatttatgga cttattgcct tttttactgg acgcgaacct
     1681 cagcgcgaca aacccacccg ctattccgca ctggtggaag cgtcaaccgc ttattcccaa
     1741 ccttctgtca caggaactga aaaactatct gaagcttaat gttaaagaga aaaatattca
     1801 gattgcagac caggtaatta ttgatgaaac tgcaggtgaa gttgttatcg gcgcgaatac
     1861 ccgtatttat catggtgccg ttattcaggg tccggtagtg attggcgcaa actgcctgat
     1921 aggtaattat gcgtttattc gtcctggcac aataatcagc aatagtgtaa aaattggttt
     1981 tgccaccgaa attaaaaatg cggttattga agcggaagca acgattggtc cgcaatgttt
     2041 tattgccgac tcggtagttg caaaccaggc atatttgggc gcacaagtac gtaccagtaa
     2101 tcatcgtctg gatgaacagc ctgtgtctgt tcgaactcca gagggaatta tcgctaccgg
     2161 atgcgataaa ttaggttgtt atatcgggca gcgttcacgc cttggcgtac aagttataat
     2221 tttgcctggg cgaattattt ccccgaacac gcaacttggc ccgcgcgtga ttgtagaacg
     2281 taatttacct gccggaactt attcactccg acaagaactt atccgtacag gagattaaaa
     2341 tgataaaacg gacgttatta gcggcggcca tttttagcgc attgcccgct tatgccgggt
     2401 taacttccat taccgcgggc tacgatttta ccgattattc tggcgatcat ggcaaccgta
     2461 atttagcgta tgctgaactg gtggcgaaag ttgaaaacgc aacgctgctt tttaatcttt
     2521 cccaggggcg tcgtgattat gaaactgaac atttcaatgc cactcgcggt cagggtgcgg
     2581 tctggtataa atggaataac tggctgacaa cccgaacggg tattgccttt gcggataata
     2641 cgccggtctt tgcccgccag gattttcgtc aggatattaa cctggccctg ttgccaaaaa
     2701 cgcttttcac gaccggttat cgctacacta aatattacga tgatgtcgaa gtcgatgtct
     2761 ggcaaggcgg cgtatcactc tatactggcc cggtgatcac cagctaccgc tatacccatt
     2821 atgactccag cgatgcaggt ggtagttata gcaatatgat ttccgtgcgt ctgaatgacc
     2881 cgcgcggcac tggttatacg caactatggc taagccgcgg aacaggcgct tacacctatg
     2941 actggacgcc agaaacacgc tacggcagca tgaagagcgt cagtctgcaa cgtattcaac
     3001 cgctgactga gcaacttaat ctcgggctga cggcaggtaa agtgtggtac gacaccccaa
     3061 ccgatgatta taacggtcta caacttgcag cccatctgac ctggaaattc tgattccttc
     3121 tgccgcccgc tatccggggc ggccttccct gccgattagc cccccccctt tcctctttgt
     3181 tttccgacca cattcaccgg ataaatttta ttctccagtg ttatatacta taggggggta
     3241 tgcattgaca tatagcatac ccccctatag tatattgcgt gcagataatg aggtgcgaaa
     3301 tgcccagtac tccggaagag aagaaaaagg tccttactcg agttcgtcgt attcgagggc
     3361 agattgatgc tctggaacgg tcgctggagg gtgatgccga atgccgtgcc atactccaac
     3421 agatcgctgc cgttcggggc gcggctaatg ggctgatggc agaagtgctt gaaagccata
     3481 tccgggaaac gtttgaccga aatgactgct acagccgcga agtcagccaa tccgttgacg
     3541 acactattga actggttcga gcctatctta aatagctgaa tctattacca tattgaggaa
     3601 gagcgagaga tgaaatcacg tgctgccgtt gcatttgctc ccggtaaacc gctggaaatc
     3661 gttgaaattg acgttgcacc accgaaaaaa ggtgaagtgc tgattaaagt cacccatacc
     3721 ggcgtttgcc ataccgacgc atttaccctc tccggggatg acccggaagg tgtattcccg
     3781 gtggttctcg gtcacgaagg ggccggcgtt gtggttgaag tcggtgaagg cgtaaccagc
     3841 gtcaaacctg gcgaccatgt gatcccgctt tacaccgcgg agtgcggcga gtgtgagttc
     3901 tgtcgttctg gcaaaactaa cctctgtgtt gcggttcgcg aaacccaggg taaaggcttg
     3961 atgccagacg gcaccacccg tttttcttac aacgggcagc cgctttatca ctacatgggg
     4021 tgctctacat tcagtgaata caccgtggtc gcggaagtgt ctctggccaa aattaatcca
     4081 gaagcaaacc atgaacacgt ctgcctgctg ggctgtggcg tgaccaccgg tattggcgcg
     4141 gtgcacaaca cagctaaagt ccagccaggt gattctgttg ccgtgtttgg tcttggcgcg
     4201 attggtctgg cagtggttca gggcgcgcgt caggcgaaag cgggtcggat tatcgctatt
     4261 gatacgaacc cgaagaaatt cgatctggcg cgtcgcttcg gtgctaccga ctgcattaac
     4321 ccgaatgact acgacaaacc gattaaagat gtcctgctgg atatcaacaa atggggtatc
     4381 gaccatacct ttgaatgcat cggtaacgtc aacgtgatgc gtgcggcgct ggaaagtgcg
     4441 caccgcggct ggggtcagtc ggtgatcatc ggggtagcag gtgccggtca ggaaatctcc
     4501 acccgaccat tccagttggt caccggtcgc gtatggaaag gttccgcgtt tggcggcgtg
     4561 aaaggtcgtt cccagttacc gggcatggtt gaagatgcga tgaaaggtga tatcgatctg
     4621 gaaccgtttg tcacgcatac catgagcctg gatgaaatta atgacgcctt cgatctgatg
     4681 catgaaggca aatccattcg aaccgtaatt cgttactgat ttcccgcagg tatacccgtc
     4741 cacttcagac ggggttctta atactctccc cgggcagccg tccgggggat taaccgtgag
     4801 ataatgactg atggaactca ttgaaaaaca tgccagcttt ggcggctggc aaaatgtgta
     4861 tcggcattat tcccaatcac tgaaatgtga aatgaatgtc ggcgtctatc tcccaccaaa
     4921 agccgcgaat gaaaaattgc cggtgctgta ctggctttca ggcctgacct gcaacgagca
     4981 gaatttcatt actaaatcgg ggatgcagcg ttacgcggct gagcacaaca ttattgttgt
     5041 tgcgccggac accagtccgc gaggcagtca tgtcgcaaat gccgaccgtt acgatctcgg
     5101 gcaaggtgcc ggattttacc tgaacgcgac gcaagcgccg tggaatgaac attacaaaat
     5161 gtatgactat atccgcaacg agctgccgga tttagtgatg caacattttc cggcaacgac
     5221 cagaaagtct atctctggtc attctatggg cggactgggc gcgctggtgc tggcgttacg
     5281 taacccagat gaatatgtca gcgtctcggc gttttcgccc attgtctccc catcgcaagt
     5341 accgtgggga cagcaagcct ttgccgcata tcttggtgaa aataaagagg cctggttgga
     5401 ttacgatccg gtgagtctta tttcacaagg tcaacgcgtt gcggaaatca tggttgatca
     5461 ggggttgagt gatgattttt acgcggaaca gctgcggact ccaaatcttg aaaagatctg
     5521 ccaggagatg aatatcaaga cgttaatccg ttatcacgag ggttacgacc acagctatta
     5581 ttttgtctcc agttttattg gcgagcatat tgcctaccac gccaataaac tgaatatgcg
     5641 ttgataatag tgcacgactg ccggatgcgg cgtgaacgcc ttatccggcc tacatttcgc
     5701 ccgtaagccg taggcctgat aagacgcgca aagcgtcgca tcaggcatct gcgcacgact
     5761 tatctatgtt taccacatca aatcatcagg cactttgaag tcggcatacg gatcgtcttc
     5821 atcctgctct tcggcactga gcgcgctgtg caacacaata ctgctggcat cacgctgagc
     5881 aattttatcg gcaacactgg cgggaataat ggcgtattca ccttcgctat tgttatcgac
     5941 caacaagcgg gcaatcgcca gacgaccatt aatcaactgc gcctgtgtga gcttatcgac
     6001 aaaaatcttc ttaatcagat tgccgtcagt gaagttaaaa ccaatatcgc cattggcaat
     6061 ggtgattcgg ttcatttcaa tgagctgctt cacctgggct ttatattctt tcgccaacgc
     6121 cgcttgtttt tgctgttcgc taagctgttt atcacgctca agctgtgcct ttttattttc
     6181 ttctaccgca gcccgagctt cacgcgcctg aacgcgcgat tttttcgccg ttctctcgac
     6241 cttcgccgct tttttgctgg tgactaatcc tgctttgagc aactgctctt gtaaggtaag
     6301 ttttgccatc tttgtttcca aacccgctga ataattttta gcgagtatac ctgtaaacgc
     6361 cgtgggtgta ttgggggcaa gtccctttcg cccctgctta ataaggcgcg tcaggcatcg
     6421 gcacacggct gcattcttga tctccgactc atcaaaataa acaccgccaa cccagcaaca
     6481 agaatacccg gtgcagaagc ggcaattacg ccgaccgtgc cagtgcctaa tgccagcatt
     6541 ttcccggcca gtaacggacc gctcatagcc cccagacgcc ctaccgccac ggctgttccc
     6601 acacctgttg cgcggatctg cgaactgtaa aacaacggtg ccagggcata caaaacgctt
     6661 tgcccacctg tcgcaaacaa ccccgcgaca aatcccgcca gcaacatacc gttaaatgac
     6721 gacaccgttc caagcgccag cagcgaagct aacatgccgc tataaatcag tagcgacatg
     6781 gttactggac gcagcttatc catcaatgcg cccaacatta acgtcccgct tgccgccccc
     6841 atttgcaggg caaacatcac ccctgccgcc tgcgatggct ggaatccttg ctccaccaaa
     6901 agtagcggta gccagttgat caacatataa accaccagca gagtgaagaa ataacacaac
     6961 cacagcagca gcgtcgcggt tgccgtttct ggcgcaaata aggcacgcag tggtggcgca
     7021 gcctgttttt cgccagcgaa aaccgctgac tccggcagcc agcgcattaa tagcggcacc
     7081 agaatcaacg gcaccacacc acctacccaa aacaccgttt gccatgctaa gtttgccccc
     7141 gcgaaaccca gtgtcgccgc cagcgccgcg ccaatgggaa caccgcaata catcaggctc
     7201 actgccgtcc cacgaaaacg tggacccgcg gcttcagacg tcagggcgat aagattcggc
     7261 aacgccgccc ccagcccgac accggtcatc agccgcgcaa agaccagtga ggggaaatcc
     7321 caggcaatcg ccgttgccag tgagaacaaa ccaaacagcg caactgagcc aatcaaaata
     7381 cgcttacgac cataacggtc tgccagcatc ccgccaacca acgcgccggg tagcaaaccg
     7441 agtattccgg cgctaaatat ccagcccatt tgcattttat cgagtgcgaa agcctgggcg
     7501 atgccacccg ccgcaatgcc agccgcctga agatccagcc cttccatcag agcgaccaaa
     7561 aaacaaagcc cgatggtcag catcaggcgg gatgaagatg atgaaggggt acgagtcgac
     7621 ataagatgaa cctcgttgcg agtgcaggtg aatcgcgact gatacattgc cagccgcgta
     7681 atcagaacta tttaatgact gcctacgggc gaaatgtagg ccggacaaga cgtttatcgc
     7741 atccggcagt cgtgtgcaga tgcctgatgc gacgctaacg cgtcttatca ggcctacaaa
     7801 ttctcacgcc acccgtaggc cggataaggt gtttacgccg catccggcag tcgtgctggg
     7861 atgggcatac aaaatgcacg ttctcggcag tcatcattat ttgttgttgc gcagatccag
     7921 cgccacgtca acgatcatat cctcctggcc gccaaccatc cggcgtttgc ccagctcaac
     7981 gagaatatcc accgcactta agccataacg cgccgccgcc gtttcacagt gacgcaggaa
     8041 gctcgagtaa acaccagcgt atcccagcgc cagcgtttcg cgatcgactc gtaccggtcg
     8101 atcctgcaac ggacgcacca ggtcgtcggc ggcatccatt aacgcataga gatcggtccc
     8161 atgctgccag cccagtttat ccgcggcggc aataaacact tccagcggtg cgttacctgc
     8221 gcccgcgccc attcccgcga ggctggcgtc gattcggtcg cagccctctt ccaccgccgc
     8281 gatagagttc gccacgccaa gactcaggtt atggtgagcg tgcatgccag tttgcgtttc
     8341 tggtttcagc tctgctttca gggcgcggaa acggtcacgg atatcgctca tgttcatcgc
     8401 accgccagaa tccaccacat aaatacaggt cgcaccgtag ccttccatca gctttgcctg
     8461 cttggcgaga ttctccggcg tggtcatatg gctcatcatc agaaaaccaa cggtgtccat
     8521 tccgagctcg cgggcatact gaatatgctg ggcggaaaca tcagcttcgg tacagtgcgt
     8581 tgccacacga accacccgcg cgccagcctg ccaggcattt ttcagatcgt gaatagtgcc
     8641 gattcctggc agcaacaacg tcgcgatttt ggcgtgcttc accacatccg ccgctgcttc
     8701 aatccattca aggtcgctat gtgcgccgaa accatagtta aagctggaac cttgcaaacc
     8761 gtcgccgtgg gccacttcaa tcgaatccac gcgggcatcg tccagtgctt tggcaatctg
     8821 gcgaacgttt tccagcgaat actgatgacg aatggcgtgc ataccgtcac gcaatgtgac
     8881 gtccgagata taaagttttt taccgttcat gccgcttctc ctgccttgcg cgccagtgac
     8941 tgggccattt tttccgctgt cgccagcgca ctggaagtca taatatcgag gttgcccgca
     9001 taggcaggca gataatgcgc tgcgccttcg acttccagcc agaccgctgt tttcagtccg
     9061 gagaattgcc ccacgcccgg taaattgacc ggtttatcct gcgggataac ttcaaactgc
     9121 acgcgctgtt tcaggcgata acccggtacg taagcctgca ccgcctcagc catttcattg
     9181 attgaggctt cgatatcatc ttgtgaagct tcgtcgctca atacatacac cgtgtcacgc
     9241 atcatcaacg gtggctctgc tgggttaagc acaataatcg ccttcccttt tgccgcgccg
     9301 cccaccactt caatggctcg ggaagtggtt tccgtaaatt catcgatatt ggcacgcgtg
     9361 ccaggtccgg cagatttact ggcgatagaa gcgataattt cggcgtaatg aacacgcgcc
     9421 acgcgtgaaa ctgccgccac cattggaatg gtggcctggc cgccgcaggt gaccatgttg
     9481 acgttcagtt gatcgacgtt cgcctcgagg ttaaccaccg gcacgcagta agggccgatg
     9541 gcagcaggcg tcaggtcaat taagcgaata tccggtttcg cttcgcgtaa agcggcatcg
     9601 tttttcacat gcgcgcccgc gctggtcgca tcaaatacaa tgtcgatatc agcaaattca
     9661 ggcatgttca tcagcccgat caccccttca tgggtggtgg cgacgcccat acgtctggca
     9721 cgcgccagac cgtcggactg aggatcaatg ccgaccatca ccgccatctc cagatgctga
     9781 ccgtgacgca aaattttaat catcagatcg gtgccaatgt tgccagaacc gataatggcg
     9841 actttacgct tactcatgac agacttcctt ttggggctgc gcttgaaaat gtcgcagcaa
     9901 ctgaacctat gccttcaata tgggcttcaa aacgatcgcc cgcattcacc gccaccatcg
     9961 ggcctaatgc cccggtaaga atgatatctc cggcgcgcag cggttcaccc agactggcca
    10021 ttttgcgtgc cagccagaca gcggcattaa gcggatgtcc caggcattcg ctgccgcgcc
    10081 cgctggaaac ctcttcgtta ttacgcgtca tcttcatggc gcagtttttc aggtctaacc
    10141 ccgccggacg ttgcgccgga ccgccgatga catacacccc gcaggaagcg ttatctgcca
    10201 cggtatcgac aaactgaatc gaccagtcgc gaatgcggct ccccaccact tccagcgccg
    10261 gaagtaccca ttcaatggcg ttatacagtt cgtcgaaggt gatatcagtt gcgggtaaat
    10321 cgcggttcaa caccagtgcg atctccgctt caatgcgggg ttgcagaaca cgggaaaaag
    10381 gaatgatttc gttatcgcca taacacatgt cggcaaataa cgtcccaaaa tccggttgat
    10441 caacgcccag ttgttgttgc actttcggat gcgtcaggcc cactttacgc cctaccacgc
    10501 gacgcccctg cacaacgtca tgttgcacat ttatgtgctg aatggcgtaa gccgcttcag
    10561 cgttatcgat accaatcaga tcgcgcagcg gtgcaatcgc ttcgccctgc tctgcggcgc
    10621 ggcgtaaatc cgccgccagt tgctcaagag tatgcttcgt catgaccatt ccttaagggc
    10681 gtgcgaggaa attcagcacc agttgattga aagcgtcggc atgttcccac tgcgcccagt
    10741 gaccgcagtc gcggaagata tgcagttcag aaccggcaat gccggacagc agacgcagac
    10801 ccgcatccat cggcacaaag cggtcgttgc gcccccagac aatcagggtt tgcgctttga
    10861 tttccgccag acgtgggcca aaatccggga actgtttcgg attagcttcc aggctcttaa
    10921 cgaagttttc caggtgatcg cggcgcgaca gcatattatt caggcgcgct tcaaacaggg
    10981 cgtcggtcaa atcgctggta tcaaaaacga agatatccat catcagtttc aggttttcga
    11041 tagtcggctg acgataaagc tgattcagtc gcttaatacc ttcggttggc atcggcgtaa
    11101 acaaactcat gccgcccgta ccgccgccca tcagcaccag tttgccgacg cgctccggcc
    11161 agttaagggt gaacgccaca gaactatggc cgcccatcga gttgcccaac aggtggattt
    11221 tggcgatatc cagttgatcc accacgcttt tcaggattcg tgcattaaga tccgatcgtg
    11281 aaccactatt aacgatcgaa tcgctcttgc cccaacccgg acaatccagc aggatcaccc
    11341 gatagcccgc ctctaccagc ggatcgatat tgcggctgaa attcgcccag ccagtagcac
    11401 ccgggccgga accatgcagc aggacaacgg tttcgtcgcc ttgtccgcag tcattaaaat
    11461 ggatgcgcag cgttttaccc gcttcttcta cattcagaaa acggctggtg gcggcttcgg
    11521 tttgtggctg ataactcatc atcttctcct gcatattcag ttctctgttc tggcgcttaa
    11581 cgagccaaat ccggcaatcc actccgggat tgggcggtaa taacgccctt cgctacgcca
    11641 gttgccaaat gcagaaatag cggcaaaagc ggcgacccag gttttgattt catgtgtcga
    11701 ctttccggca atggcggaaa gctcttcgtt actgacggca tccagttcct gtatgcgtcc
    11761 ctgctccagc aaagtcatga actggttatc ccaaatcggg ttgagcggat gcagagttct
    11821 ctgatcctca acaaacttct cagcggcgct aatcacccgt tgctgacgca attcgcgctc
    11881 actggcgggt aaatctttcc cgctccccaa cagacggtcg cgcatatggg catcggcttt
    11941 cgccagttcg ggcaccggcg gctgatggga aagcccaccg gaacccagga acagcacgcg
    12001 tttattgaga gtgctggtga aacgtccaat ggcttcaccc aacatgcggg tacgctggaa
    12061 accgggcagc ggcgtggcga caccgttgat gaacacaggc agaactggca ccttatccag
    12121 cccaccgagc aggaactcca gcggctgggc gaacccgtgg tccacctgca tacagtaaga
    12181 aacggcaaga tcgatcccgc tcttcatgac ggcatgcgca caggcctccg ccagctccac
    12241 aggcacgggc agctctcctg ccgcactgcc gaaatcacca attgccgtcg ctccaacgcc
    12301 taaacagaac ggtggcatca cgtcatagaa aaagccgttg tagtgatctg gcgcaaacag
    12361 caccaccagt tcaggggaga aagccgcaat acgctcgcgg gcgctggcaa tcacgccatt
    12421 gacctcatcg agtacctctt gtgccgggtc gacatacccc accagcggcg agtgggaaag
    12481 acaatgaaga taagcgtgca tatcaggcta ccttttcgac agaaacgtcg gcatcagggc
    12541 gggtcagcgt catcaccgac gccagtttat tcagggtctt gcccagggtt tgcggaatgg
    12601 cggtggcggc aacaaagcga tccgggcgca tcaccaccag cgaagcattg tgttgtgcga
    12661 accagctacg cagacgacct tgcgtatcgc ccacgcgtag cacgccgtcg tggttatcct
    12721 gtgccgtatg aatttgcact tccggcacca cctgaataaa gcgtgtgccc aacgcgcgcc
    12781 actgctggat ttgctcgtcg ctcatccccc acagtggatt gcatccccag ccaattaccg
    12841 cgaagttcgc gccgatcgcg ttatcgagca gcgtcacgtc gccgttttcc agcgtgactt
    12901 tcggctgaat aaacatcttg ccgaccggag agtgcttcgc ttcgccctca cgtaccagcg
    12961 caccgccgta atattgcggc atcggcttga agcgcatttc gaggaagtag cgttttactg
    13021 gcggcagata attcagcagc caggaaacgc cgtcacgtaa cgtaccctgc cagcgtttcg
    13081 gtggagccag cacgttgccc gccgtcacgg acaggtcaat catcgctttg gcgtgatcgc
    13141 gacgttcttg ttgataggta tcgagcagcg catcgcgggc tttcccctgg ataaccaacg
    13201 ccagtttcca tgcgaggtta aaggcgtcgc gcataccact gttatagccc tgcccctgcc
    13261 ataccggcat gatgtgcgcg gcatcgcccg ccagcagtac gcgatcaata cggaaacgtt
    13321 gcgccagtcg cgcgttgtgg gtgtagacac gctggcgaat caattcaaca ttgtccggat
    13381 taggcagcac tttgcttaac agcttgcgca tattttgcgg ctcacgcagc tgctcttcgg
    13441 tttctcccgg catcaccata aattcaaagc gacgtaccgc atgaggcagc gcggcagaaa
    13501 catacgggcg caccggatcg caacacaaat agatatgcgg cgtacttaac ggatcgttgg
    13561 cgatatctac cacaatccac tgatttggcg cagttttacc ttcaaacggc acattcagag
    13621 tgcgacggac aaaacttgct ccaccgtcac aggctaccag ccactgggct ttgaccgttt
    13681 cccgctgccc ttctgccgtt ttcaggtgca aggtcacttc gtcattttgc tggctgaagg
    13741 cctccagctc gcgggcaaac aagcagcgca cattcggaaa acgcgacaac ccttccagca
    13801 tcaccgcatc gacctgtggc tgaataaagg cgttacggcg cggccagcca aattcatcgg
    13861 tcattggctg aatatcagca aaacagcggc cttttggggt gagaaaacgc atcgcgtgcc
    13921 acggtgtagt gtgcggcaga acattctcga ccaggccgac cgactgcatg gtgcgcagcg
    13981 cctcgtcatc aataccaatc gcacgcgggt agtcgatcaa cttatcgagt ttctccacca
    14041 ccagcacgtc aatgcccatc tgaccgagat agttcgccat catcagccca accggaccgg
    14101 caccagcgat cgccacctga acgctatggt taacagcagg ctggatgtca gggtgttgta
    14161 ttgccatttc agtacctcac gactcggaca aaatgccgtt gcgcgcacag tacagcgcaa
    14221 cttattttgt taaaaacatg taaatgattt tttattgtgc gctcagtata ggaagggtgt
    14281 tttcggctac aatcaaaaca tgctcgaatg tgcaccaggt gcacaacgtt gtttaaacta
    14341 tagaaatgtc aattaatatg cagaacaatg agcagacgga atacaaaacc gtgcgcggct
    14401 taacccgcgg tctaatgtta ttaaatatgt taaataaact tgatggcggt gccagcgtcg
    14461 ggctgctggc ggaactcagc ggcctgcatc gcaccactgt gcggcgactg ctggagacgc
    14521 tgcaggaaga gggatatgtc cgccgtagcc cctccgatga cagttttcgt ctgaccatca
    14581 aagtgcggca attaagcgaa ggatttcgtg acgaacagtg gatttctgca ctggcggccc
    14641 cactgctggg cgatctgttg cgcgaagtgg tatggccgac agatgtgtcc acgctggatg
    14701 ttgatgcaat ggtggtacgc gaaaccaccc accgtttcag ccgcttatct tttcaccggg
    14761 caatggtcgg gcgacgtttg ccgcttctga aaaccgcctc gggcctgacc tggctggcct
    14821 tttgcccgga acaagaacgc aaggaattaa tcgagatgtt agcctcccgc cccggtgatg
    14881 actatcaact ggcacgagaa ccgttaaagc tgcaagccat tctggcgcgc gcgcgcaaag
    14941 agggttacgg acagaactac cgcggctggg atcaggagga gaagatcgcc tctatcgccg
    15001 taccgctgcg cagtgaacaa cgggtgattg gctgtctgaa tctggtgtat atggcgagcg
    15061 caatgaccat tgaacaggcc gcggaaaagc atcttccggc gctacaacgg gtagcaaaac
    15121 agatcgaaga aggggttgaa tcgcaggcta ttctggtagc cggaaggcga agcggcgtgc
    15181 atttacgttg acaccatcga atggcgcaaa acctttcgcg gtatggcatg atagcgcccg
    15241 gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgctatacga tgtcgcagag
    15301 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct
    15361 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc
    15421 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg
    15481 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt
    15541 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg
    15601 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag
    15661 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct
    15721 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg
    15781 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct
    15841 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag
    15901 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa
    15961 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg
    16021 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg
    16081 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag
    16141 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
    16201 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg
    16261 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
    16321 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
    16381 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtgaaattgt
    16441 gagcggataa caatttcaca caggaaacag ctatgaccat gattacggat tcactggccg
    16501 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
    16561 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
    16621 aacagttgcg cagcctgaat ggcgaatggc gctttgcctg gtttccggca ccagaagcgg
    16681 tgccggaaag ctggctggag tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa
    16741 actggcagat gcacggttac gatgcgccca tctacaccaa cgtgacctat cccattacgg
    16801 tcaatccgcc gtttgttccc acggagaatc cgacgggttg ttactcgctc acatttaatg
    16861 ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggc gttaactcgg
    16921 cgtttcatct gtggtgcaac gggcgctggg tcggttacgg ccaggacagt cgtttgccgt
    16981 ctgaatttga cctgagcgca tttttacgcg ccggaaaaaa ccgcctcgcg gtgatggtgc
    17041 tgcgctggag tgacggtagt tatctggaag atcaggatat gtggcgaatg agcggcattt
    17101 tccgtgacgt ctcgttgctg cacaaaccga ctacacaaat cagcgatttc catgttgcca
    17161 ctcgctttaa tgatgatttc agccgcgctg tactggaggc tgaagttcag atgtgcggcg
    17221 agttgcgtga ctacctacgg gtaacagttt ctttatggca gggtgaaacg caggtcgcca
    17281 gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg tggtggttat gccgatcgcg
    17341 tcacactacg tctgaacgtc gaaaacccga aactgtggag cgccgaaatc ccgaatctct
    17401 atcgtgcggt ggttgaactg cacaccgccg acggcacgct gattgaagca gaagcctgcg
    17461 atgtcggttt ccgcgaggtg cggattgaaa atggtctgct gctgctgaac ggcaagccgt
    17521 tgctgattcg aggcgttaac cgtcacgagc atcatcctct gcatggtcag gtcatggatg
    17581 agcagacgat ggtgcaggat atcctgctga tgaagcagaa caactttaac gccgtgcgct
    17641 gttcgcatta tccgaaccat ccgctgtggt acacgctgtg cgaccgctac ggcctgtatg
    17701 tggtggatga agccaatatt gaaacccacg gcatggtgcc aatgaatcgt ctgaccgatg
    17761 atccgcgctg gctaccggcg atgagcgaac gcgtaacgcg aatggtgcag cgcgatcgta
    17821 atcacccgag tgtgatcatc tggtcgctgg ggaatgaatc aggccacggc gctaatcacg
    17881 acgcgctgta tcgctggatc aaatctgtcg atccttcccg cccggtgcag tatgaaggcg
    17941 gcggagccga caccacggcc accgatatta tttgcccgat gtacgcgcgc gtggatgaag
    18001 accagccctt cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg ctacctggag
    18061 agacgcgccc gctgatcctt tgcgaatacg cccacgcgat gggtaacagt cttggcggtt
    18121 tcgctaaata ctggcaggcg tttcgtcagt atccccgttt acagggcggc ttcgtctggg
    18181 actgggtgga tcagtcgctg attaaatatg atgaaaacgg caacccgtgg tcggcttacg
    18241 gcggtgattt tggcgatacg ccgaacgatc gccagttctg tatgaacggt ctggtctttg
    18301 ccgaccgcac gccgcatcca gcgctgacgg aagcaaaaca ccagcagcag tttttccagt
    18361 tccgtttatc cgggcaaacc atcgaagtga ccagcgaata cctgttccgt catagcgata
    18421 acgagctcct gcactggatg gtggcgctgg atggtaagcc gctggcaagc ggtgaagtgc
    18481 ctctggatgt cgctccacaa ggtaaacagt tgattgaact gcctgaacta ccgcagccgg
    18541 agagcgccgg gcaactctgg ctcacagtac gcgtagtgca accgaacgcg accgcatggt
    18601 cagaagccgg acacatcagc gcctggcagc agtggcgtct ggctgaaaac ctcagcgtga
    18661 cactccccgc cgcgtcccac gccatcccgc atctgaccac cagcgaaatg gatttttgca
    18721 tcgagctggg taataagcgt tggcaattta accgccagtc aggctttctt tcacagatgt
    18781 ggattggcga taaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc cgtgcaccgc
    18841 tggataacga cattggcgta agtgaagcga cccgcattga ccctaacgcc tgggtcgaac
    18901 gctggaaggc ggcgggccat taccaggccg aagcagcgtt gttgcagtgc acggcagata
    18961 cacttgctga tgcggtgctg attacgaccg ctcacgcgtg gcagcatcag gggaaaacct
    19021 tatttatcag ccggaaaacc taccggattg atggtagtgg tcaaatggcg attaccgttg
    19081 atgttgaagt ggcgagcgat acaccgcatc cggcgcggat tggcctgaac tgccagctgg
    19141 cgcaggtagc agagcgggta aactggctcg gattagggcc gcaagaaaac tatcccgacc
    19201 gccttactgc cgcctgtttt gaccgctggg atctgccatt gtcagacatg tataccccgt
    19261 acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat tatggcccac
    19321 accagtggcg cggcgacttc cagttcaaca tcagccgcta cagtcaacag caactgatgg
    19381 aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac atggctgaat atcgacggtt
    19441 tccatatggg gattggtggc gacgactcct ggagcccgtc agtatcggcg gaattccagc
    19501 tgagcgccgg tcgctacca
//
LOCUS       DAPHBJ010000077        19336 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.077, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000077 DAPHBJ010000000
VERSION     DAPHBJ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.077"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            317..3223
                     /locus_tag="RHZ76_002412"
     CDS             317..3223
                     /locus_tag="RHZ76_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDV2813042.1"
                     /translation="MAETVSLTPAKLYEESTVFFRSPESGLWCRGRYGGQRPDNKHLV
                     IVRSDKNVVIDLADIFYPNYGQGLAMNPAHFLAARANDAPFFYPLREKFVSAWIKQRA
                     ACRSMSSLISSRVEIEPHQIAIVRRILQDQNPKYLLADEVGLGKTIEAGLVIREHILE
                     CKREARVLVVVPQVLQGQWTQELVDRFALQEVMSGGRHTQIRLCLPEELCQPELIGWK
                     PTMVVIDEAHQLTRQAWADDEDDLAQYAAMAALCHQARIVLLLSGTPMHGNERHFLAM
                     LHCINPQAWQLNAQGIEKFMQRVAERERLGGIYSSLTPETPNGVLEEILEELRLLFAD
                     DEQLQARIVGLSPLVDFFAPDESEERTAGILALRQWIGERYRLFHRLLRNRREDPALE
                     YLFPGLAGLEKCVWPVASTEITLDETLDAWRAAAWRNPAQHQHLDSTTLDEWVEALFL
                     NPLVMSRQAKKALVKGTASEEEFNFLEQIIETSQQEQKAKDVALMKALTDWFEKCPDG
                     KAVIFCGEGSEAAFLFTKLQIQAPWAVVRHAPDQRKQSELLDDSWQVLICDRRGEDGL
                     NLHGNNRLAVHYSLSRDFNRFEQRLGRFNRYSGNLRGVKPVKSLVLLPERDGLRADWV
                     KLLDEGTGLFHRSVASLQFVLSEQLDVVWRDYVGQGLAVFHEAQQRFSGENGFIAQER
                     KRVLAQENLLSMEQEVIAAREFSEQLAESEDDAEEQAKDMLAWMCKALGFKREKYPEG
                     GFRLRFERGEYQRQTLVDVGTFIDNCLLGLDFSEGYPPSTAMMSLSRTEAGNHKHVYP
                     LRYGQPFVETVWQLMQSDPRGASMALLRVLSSAVALKQPHTWFHFQWLSEAQVEGENQ
                     LAAQRRGDECFSPVVHNFWLDDGGKEADPQIVVSLLDKPYDEEGNRLFQDINLREEVW
                     TRMPDWFDPHSWKETVLAAAEQARQNVHAHYGDRPVRHQLLAMKAIILCTRDML"
     gene            3236..5686
                     /locus_tag="RHZ76_002413"
     CDS             3236..5686
                     /locus_tag="RHZ76_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDV2813043.1"
                     /translation="MENQWQQLRAVLNGELNERDGLSGQFFNRLLYCLDDVRAGECDR
                     LHAFYDALQCARALGLTPLRLPYRHLTEGTMLQHFGLRRDHDYPGELLLTREAEEIDP
                     HLLPVWKGEQRRFLETPPLDVLLPSVLNDPRYTHYTSAGQQQAVRTVLTCGRDKTLLV
                     NLPTGSGKTFVIHSQMLTSPRRHLTLVIVPTVALAIEQALRAQEVMQQAGQDHGGRYV
                     WHSGQSTEERNEMKSRLASGEQRALFCSPEAATGGLLLQLFSLAERGLLGAVIVDEAH
                     LIDQWGAEFRPEFQLLAPLVRSLKATSPDGVKVVLLSATFSQSTLNTLKALFAGKRQG
                     SVIEVNGSFLRPEPVWYVSEAASHEDHLAQVENAIARLPAPMILYTTEVEQAKFWYQY
                     LRERGYRRCGLFHGATPMQEREGLIHAWRNDGLDIMVATSAFGVGMDKNNVRSVLHVA
                     VPENLDRFYQESGRGGRDGKASVAHIIFHHKQLQVARNINRTKLIGASKGYLRWAKMH
                     QLREQHQPGQFIVPLRAKHADIRMDSQSNVDWNLRTLLLMQRAGFIDIAYPPPDLSAI
                     APDERDEAKIRAWFDHYFNHIQVSVRRDGHMDEARWHEGIQAHRSHELGMRKQGYSAL
                     EEWLNDPSISLCQKLAQFYTLDGFVPEIACGGCPACRSKGYPPFTPTLGRIAHVTGEA
                     VRNVMGNEQRVYYPATLTNRLLLRQWSDWIARLLANRQIQAIRASQSVLAQLSAVLPA
                     GLPFWCSLAVDEENTCWDELVLVLPDEAMPELDIFATINRIIVAPERLQEPGYRGRRW
                     WEVDTSAVALEQFQRNII"
     gene            5686..6585
                     /locus_tag="RHZ76_002414"
     CDS             5686..6585
                     /locus_tag="RHZ76_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813044.1"
                     /translation="MAITNNAAAGSQIRLLCMIDRVLNRRTGQAIPMVDLIELLRPEM
                     LPVSDGARKRLPAEVSFWTKEGLWKNESAGLSQQSPLSSERNLPARVLRTLINSEKTT
                     PLLTGTRGQPLLMCVTSMLAQEKYTLRGSTPLTKEAVPLAVGPMINNKLAGEGRRNLN
                     VSNEAESFLNYAYFLGFTEPYLNGWVMDPTRAIEGILDNLGLTAGMPIQEFLSQLAEF
                     LPMLDGGAYRQQVEPMISADNWQPLEKHMISASLSQALLRLELTMQLVFSSRSDDLDA
                     MVLQGPDGSLRRISTVSPGEARK"
     gene            6588..9731
                     /locus_tag="RHZ76_002415"
     CDS             6588..9731
                     /locus_tag="RHZ76_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813045.1"
                     /translation="MIEYWPSLKNINECIRTEAEELAEYTLLAVHQPVNLLRRDKDGN
                     NLGYAKEEDLLNHFLETPRPIPVVGKAGVGKSHIIRWLDAKLRLRQEYKDKKWHIVRI
                     PKSASLREVLTSMLAGLEGEIFDEAREDINKVSDKRTPREIAEWLLMLMGQELRNLHE
                     RNKQDMEALKKQMAGSTPEQLKAMKPQATRLQKIHIHAEDNALPTLINDAYFKQFLLK
                     EEHCLYRLASRLTNGATSSDLDEGEQQLQAKDLDFSFNINDLSLPARQYIQKSRLNTH
                     EDGRKDASEILNLVLGKATQALFNQLFNFRGRSFSDLFTQIRQALHEKHMTLMVLVED
                     MSLITAIEDVLIDSLEREGIRDGKEVLCPVCSAIAVTDGYQGYARRRQGMLDRAKGEW
                     VIEEVGSGREETRLRIVDFCGRYINAARFGSEALLQRWEQAADKSHWPPDWEASANGD
                     EMAEVFGRSEQGFSLYPFNASAIHALAEAFCRDDRNELKFNPRQIINQILLRVLQHCR
                     RDADEGRFPPPRLGDIAAPAGLRSWLFRQGFADTERAESVVALWGYPADSDATLASTL
                     PPDVARSFDLEDLAQVLENTKSAPLVEQSVMPTRVTKVEQQVKKTVQPPKPVKPVLKE
                     DKDTLAIRALDAAVGDWMLKGAPLEIDPARYIKSSLAFFFDKRAVAEWAGSSYRPTLW
                     LGKSNFVAVELPNAQGNRGVHVVKFISQSEYDKRSVQLTDAAMALARFGYYRENSLTK
                     TEDWSYPEGKTDYLIIQSFCDRWVNYALTELVKHKRNDLPLLLSEQIALADALGVIKT
                     ADGPKEVLGRLLQNSKTLSSQFRTGITKAITELRGEALAKWDDAQDAWLSLVALNDHA
                     LEGDLLLSAIQKVLKKRSNNTHAAVVKKSLSEIRPALDTAALFADCENADDFSELVTG
                     LTQLVKSLGDSGDYPADMTPDSSTLANSLTGLTEGGVWLTILKLRGITQSEDPLRQWQ
                     LLCELDGTLINRLIFTISGWQQVNKRVLANITAYNHSHGSHQISEFRTQIESTLQELH
                     QVLDAMQSVAGEQYDNA"
     gene            9718..10452
                     /locus_tag="RHZ76_002416"
     CDS             9718..10452
                     /locus_tag="RHZ76_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813046.1"
                     /translation="MTTLKTQLTQLREQYQIKQQVQSLEEVYDRYRTIRDGLLDVAEP
                     LQKAQKQLEVIGTLPEPHSAIDFSQEAALFGGAKTQLDALTARFKESGDKTEQCGFWE
                     TVRVLKSVHESIDDKVDATWKAFVADLEARATLDPVFLEQQRTLGNVLVYDDYRKARD
                     NFNRQKLSGPDSLSVVKNLQKYSDEMIALKRSMDLDAPDDVLRFFEALDRHNHASLTL
                     LTPGVITWLEEKDMLPAFNVTRKRMI"
     gene            10452..14966
                     /locus_tag="RHZ76_002417"
     CDS             10452..14966
                     /locus_tag="RHZ76_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001497987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDV2813047.1"
                     /translation="MKGIDLSLLQRVLDALEEQENALLVWGDTGGFFNLDELLSVIGR
                     VLPDEDAEVVLDQMLEAAMLIQVPHVSGTPVWRTRMGETVHLMRNLRQWMHKQKLDQS
                     KTLVSDYRFIRRPRSYPDRVYEPATLISGWKKQLKLSDRVCDIMRQALASDKPFLLAG
                     FQVRATERIMQCWQDHQVKSNTASGTIVCAGTGSGKTLSFYLPALTRLAEDICNNPER
                     KVRILAIYPRKELLKDQFAETFSQCRKLDDYMLTAAGRKIRIGAFFGDTPVKAEWSKE
                     DVKGKVGLPFGLMKCQQPHPHHPKQRCGGALIWRREDVFGEREVLTCTQCQHQVDQSE
                     IMITRDAQQHRGDAPDILFTTTEMLNLQMNSTWSNHLFGVGEGYGPTLVLLDEVHTYS
                     GTTGAQTAFLLRRWMQRTDCLPHFVGLSATLTDAQHFFARLVGAAEEQVALIQPYMED
                     MIEEGAEYLLALRGDPVSETALLSTTIQASMLMARMLDSKANKSQGTWGKKTFIFTDT
                     LDGNNRLYHDLSDAEGWETGPGHSRIDHSPLAVLRSPFDNTAPERSKTELGQNWRAAT
                     DIGHDLAENKVISRTSSQDAGVDASADVVVATSSLEVGYNDPLVGAVLQHKAPNDVAS
                     YLQRKGRAGRPRGMRPWMLVVLSEFGRDRVEFQRYEGLMSPEIKRQGLPLDNQHVQKM
                     QAAMATLDWISKVGQFKDLWSMLKKAEHNQLKYNRMYGPLIKLIEEVLSGGRRLNELM
                     RYLQDALQLSDGAVQNILWSPPRSIMFEFLPTILRNLRTRWSVNGVEWAGLRPNQPNS
                     EGEQHRSNSPVPEYIPQNLFSELNLPELDIRLKRGFDDEDHWETLSFWQGIREFAPGR
                     LSKRYAVKSNKSTDWLVPQSYEPMAGEGRQFVDFQISDAFGDSWQNEYEVDYMGKTIK
                     VVKPSKVMTTRADIRRINDKSNAQLQWVFNVINPAIATPDEVPKGPWKHTLSDVTFYN
                     HQHMTPLELVRFSTGSQASLRFRNKERAHVDFTWVNGEEQVGVGSRQWVDAMRLRFNL
                     TCDDVLGLLHQEEIQRGMRPVYFQHLVRQSPEFEFDSFNADWAIECFMAQLAETLANG
                     AHASVESALREMASEKGGERLADIPASLFQPDTDNETGTDQALQIGLNKLLQRPEIQQ
                     LLLNCAQALWKPLDEIDGFVEWARQVLADTLAAGVQQTLSTLLPDVDERAVVTDSSWM
                     SDPRKGAEWLEIWLCEMESGGSGILIRLQQKWAEDPVSFLNVLVRNLSASDYEQIDYD
                     LRTVLQMMQTDDTLRMAISAVREASNMDARREANKNLHLQLSQRGMRLSHSFTTVLYS
                     RILRAGSGEHTDTQLYQLLSDWSSLEASAGIEFSMNTMAHALAVNSLGVETDASVVFD
                     EQCRNQNLLWPRGYTIRQAELGFYNMFCSRKVTTERLLAGALFSEQIEKIALDDDWLG
                     HLHAALRKGGRAELILTRQQRNQLHQVITTVQIEPVDHLGLLLYPRLGEVRREQDVLI
                     LRIELAEAMQ"
     gene            14963..15508
                     /locus_tag="RHZ76_002418"
     CDS             14963..15508
                     /locus_tag="RHZ76_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD-like domain protein"
                     /protein_id="HDV2813048.1"
                     /translation="MIGRAKQRQIFLHGSAGHRQVREVLSAQVVALIMQPEPIWLVSP
                     WVSNFSLLDNRSGNWDSIDPAWGGREVDFIELLACAVNNGAPLSLVTRDEPMNRIFVK
                     ALEKRLSNFATFRLEWRNQLHTKGFLTPNFFLKGSMNFTRSGVNRNEEFVELNCDSHM
                     IGEAMAEFESQYLFIGSKEAF"
     gene            15508..17679
                     /locus_tag="RHZ76_002419"
     CDS             15508..17679
                     /locus_tag="RHZ76_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813049.1"
                     /translation="MCADWLKNYYAEDNYLDYDKAMQGGYGIPQMNTLIQQAAALRMP
                     CIVPSTRTGRTVFYALAENAKSLDELRRILTAALGSADTTPDIKSLNHSDDGGEQLLL
                     EKSPDGFLAFDFLPVPDGSPQQVKEWQAARMKRVYAMLQTVMDLYHQRPVLHSLVSRQ
                     TGRILRDFYTACQARDGKIAEQYLEELRGNQTLSSLNLLFLELQGMAASAKWDAILNH
                     PRLEVLLRGRVPERIQRLLLRSSGHLMLNAIRDAHFPLDRREDARRLVLGLLPLYKHK
                     PRFAHQASFLPDWQLWAMGAALLGIDEWQTATPLLAPDWIQQVEGWATGTTSLPAPVA
                     AEEQALIQAPVIMLISLENATDLLLEALLADAERESEIYAQLAAMPEATRQALQKIPK
                     LWEAWQALKKRCEPQDYGWCRWLADLQQTTESERFESLRQQATVHYMDWAPSTFSETQ
                     WQALLEHQSNTQLSKVLRDVLPTLLNWLEEYDVKVSASLWPDWLMLLAVEDIRSEEDV
                     RLGGMILDKFLSSPFTCEEYSAAIESTEILCAENVSVRTLGYSLDIAELLYDRVTANE
                     ASRLGFWIKLQELLKHRWERLDASMQFSARMVERLYLGEHAGNVFPSEDNTPGVASSL
                     HRDLSGKTLAIYSLMKGAARRGKEALLQLYPGLEVELNHDHVATPALVNLAKTADYFI
                     FASSSSKHQAFYAVTDYRKEIIYPSGKGASSMIASFVRAME"
     gene            complement(17942..18304)
                     /gene="queD"
                     /locus_tag="RHZ76_002420"
     CDS             complement(17942..18304)
                     /gene="queD"
                     /locus_tag="RHZ76_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDV2813050.1"
                     /translation="MKTTLYKDFTFEAAHRLPNVPNGHKCGRLHGHSFVVRLEITGEV
                     DKHTGWIIDFAELKAIFKPTLDRLDHYYLNDITGLENPTSEVLAEWIWNQIKPQLSIL
                     SAVIVKETCSAGCIYRGE"
     gene            complement(18555..18944)
                     /locus_tag="RHZ76_002421"
     CDS             complement(18555..18944)
                     /locus_tag="RHZ76_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813051.1"
                     /translation="MFAKSFIALDGNDRLTGARTAQTAPYDRYTCHLCHSALKYHPEY
                     DTERPWFEHIDAGSAENGQHNCPYVKPDASEIHLARRLQRFLPDATPRVYKADWNCIQ
                     CGKNYYGECYCSACRTGDFSQLTKMGS"
     gene            complement(19115..19207)
                     /locus_tag="RHZ76_002422"
                     /pseudo
     CDS             complement(19115..19207)
                     /locus_tag="RHZ76_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547754.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
     gene            19221..19336
                     /locus_tag="RHZ76_002423"
                     /pseudo
     CDS             19221..19336
                     /locus_tag="RHZ76_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         4641 a         4807 c         5477 g         4411 t
ORIGIN      
        1 ataattgtga tcgctgtcac tctgcaagat aatgatgaga aataatttat ctcctgatta
       61 caattgctaa aaaatcattc ccaccatata ttaaattaca tgcatacaca gcgatattgt
      121 tcgcatcgca atatgatgct attcgctaca gctaataaaa aattaaatga ctacactacc
      181 caatacattc tctgaaggtg atctagttac ttctcttttg tttggcggaa tcgccaaaat
      241 tgtacgtctt cacccgcctg gcaaggctga gatcgcctgg tttgtctcac ctctcgaata
      301 cgaggttaac cgccagatgg cggagaccgt atcactgacg ccggcaaaac tgtatgaaga
      361 gagcacggta ttcttccgct cacccgagag cggactgtgg tgtcgggggc gttatggcgg
      421 gcaacgtccg gataataagc atctggtgat cgtccgctct gataagaacg tcgtcatcga
      481 tctagctgat attttttatc caaactacgg acagggactg gcaatgaacc cggcccattt
      541 cctcgccgct cgcgccaacg acgcaccttt tttctaccca ctgcgcgaga agtttgtaag
      601 tgcatggatc aaacagcgcg ccgcctgtcg gtcaatgtcg tcgttaatca gcagtcgggt
      661 ggagattgag cctcatcaga tagcgattgt ccgccgcatt ctccaggatc agaaccccaa
      721 atacctgcta gcggacgaag taggactggg caaaaccatc gaagcgggac tggtcatccg
      781 agagcatatt ctggagtgta aacgtgaagc ccgcgtattg gtggtggtgc ctcaggtatt
      841 gcaaggccaa tggacgcagg aactggtcga tcgttttgcg ctacaggaag tgatgagcgg
      901 aggacgacac acccagattc gtctgtgcct tccggaagaa ctttgccagc cggaactgat
      961 cggctggaag cccacgatgg tggtgatcga cgaggcccat caactgactc gacaggcatg
     1021 ggccgatgat gaagatgacc tggcgcagta tgccgctatg gccgcgttgt gccatcaggc
     1081 ccgtattgtg ctgctcctgt ctggcacacc gatgcacggt aacgagcgtc acttcctggc
     1141 aatgctgcac tgcataaatc cacaggcgtg gcagcttaat gcacagggaa tcgaaaaatt
     1201 tatgcaacgc gtggcagagc gcgagcgtct ggggggaatt tacagctctc tgacccctga
     1261 aacaccaaac ggggtgctgg aagagatcct cgaagagtta cgtctgcttt ttgctgatga
     1321 tgagcaactc caggcgcgca ttgttggtct gagtccgttg gttgattttt ttgcacctga
     1381 tgaaagcgaa gagcgaacgg cgggcatttt ggcattacga cagtggatag gagagcgcta
     1441 ccgtcttttt caccgcttgc tgcgtaaccg acgtgaagat cccgcgctgg aatacctatt
     1501 tccaggtctg gccgggttgg aaaaatgtgt ctggcccgtc gcatcgaccg agatcacgct
     1561 tgatgaaacc cttgatgcct ggcgtgccgc ggcctggcgc aaccccgcac agcatcaaca
     1621 tctggattcc acaacgcttg atgaatgggt ggaagcgctg tttctcaacc cgctggtgat
     1681 gtcccgccaa gccaaaaagg cactggtaaa agggaccgcc agcgaagaag agttcaattt
     1741 tcttgagcag attattgaaa cgtcccagca ggagcagaaa gccaaagatg tggcgctaat
     1801 gaaggcgctg accgactggt ttgaaaaatg tcctgatggc aaagcagtca tcttttgcgg
     1861 tgaaggatca gaagccgcct tcctgtttac caaactgcaa attcaggccc cctgggcggt
     1921 agttcgccat gcgccggacc agcgtaagca gagcgagttg ttggatgaca gttggcaggt
     1981 attgatctgc gataggcgcg gcgaagacgg actgaatctg cacggaaaca accggcttgc
     2041 ggtccactat agtctgagcc gcgactttaa tcgctttgaa cagcggttgg ggcgctttaa
     2101 ccgctattcc ggaaatctac gcggcgttaa gccagtgaaa agcctagttt tactgcctga
     2161 gcgtgatggg ctgcgtgctg actgggtgaa attactggac gaaggcacgg gattgtttca
     2221 tcgaagcgtc gccagcctgc aatttgttct gtctgaacaa ttagacgtgg tatggcgcga
     2281 ttacgttggg caggggcttg ccgtcttcca tgaggcgcaa cagcgctttt cgggagaaaa
     2341 tggcttcatc gctcaggagc gtaagcgcgt tctggctcaa gaaaacttgt tatcgatgga
     2401 acaggaggtc attgctgccc gcgagttttc tgagcaactg gccgaatctg aagatgatgc
     2461 cgaagagcag gcaaaggata tgctggcttg gatgtgcaag gcattagggt ttaagcgaga
     2521 aaaatatcct gaaggcggat ttcgcctgcg cttcgagcgt ggcgaatatc aacggcaaac
     2581 gctggtggac gtgggaactt ttattgataa ctgcctgctc ggtctggatt tttcagaggg
     2641 atatccaccc tccacggcga tgatgagcct gtctcgtacg gaagccggca accataaaca
     2701 tgtttacccc ttgcgctacg ggcagccttt cgtcgaaacc gtatggcaac tgatgcagag
     2761 cgatccgcga ggtgccagta tggcgctgct tcgggttctc tcctccgcgg ttgcgctgaa
     2821 gcaaccccac acctggttcc attttcagtg gctgagtgaa gcacaggttg aaggtgaaaa
     2881 tcagctagcc gcgcagcgtc gtggtgatga gtgcttcagc ccagtggtcc acaatttctg
     2941 gctggatgac ggtggaaaag aggctgaccc gcagatcgtc gtctccctgc tggataaacc
     3001 ttacgatgaa gagggcaacc gtttgttcca ggatatcaat ctgcgagaag aggtctggac
     3061 gcgaatgccg gactggtttg atccgcatag ctggaaagag accgtactcg ctgcggcgga
     3121 gcaggcccgg cagaacgtgc atgctcacta tggtgatcgg cccgtccgcc atcaattgct
     3181 tgcaatgaag gcgattattc tgtgtacccg tgacatgctg taatcggaag caacaatgga
     3241 aaatcagtgg caacaacttc gtgctgttct gaacggagaa ctcaacgaac gcgacgggct
     3301 gagtgggcag ttttttaacc gcctgcttta ctgcttggat gatgtacggg cgggagaatg
     3361 cgaccgcctc cacgcatttt atgatgcact gcaatgcgct cgggcactcg ggctgacgcc
     3421 gttaaggttg ccgtaccgac accttaccga aggcacaatg ctccagcatt ttggtttgag
     3481 acgggatcat gactacccag gcgaactgct cctgacgcgc gaagcggaag agattgaccc
     3541 ccatctatta cccgtctgga aaggcgagca gcgccgtttt ctggaaaccc caccgctgga
     3601 tgtacttttg ccctctgtac tcaacgatcc gcgttatacg cactacacgt cggcagggca
     3661 acagcaggcg gtacgcacgg tgttgacctg cggcagggat aaaacgctac tggtgaatct
     3721 gcccaccggg tctggtaaaa cctttgttat tcactcccag atgttgactt caccgcgaag
     3781 gcacctgacg ctggtgattg taccaactgt ggcgctggcg atagaacagg cgctccgggc
     3841 gcaagaggtg atgcaacagg ctgggcagga tcacggtggt cgttacgtct ggcatagtgg
     3901 ccagtcgaca gaagaacgta atgaaatgaa gtcgcgactg gcatccggcg agcagcgagc
     3961 gttgttttgc tcccctgaag cagcgacagg tgggctcttg ttacaactgt tctcgcttgc
     4021 ggagcgcgga ctactgggcg ccgtgatagt ggatgaagcg caccttatcg atcaatgggg
     4081 ggcggagttc cgtccagaat ttcagctact ggcgccacta gtgcgatcgt tgaaggctac
     4141 ctcgccagac ggtgtaaaag tcgtgctgct ttctgccact tttagccaaa gcaccttgaa
     4201 tactctcaaa gcgctctttg caggtaaacg ccaagggtca gtgattgaag tgaacggcag
     4261 ttttctgcgt ccggaaccag tgtggtatgt cagcgaggct gccagccacg aggatcatct
     4321 tgcacaggtg gaaaacgcaa ttgctcgctt gccggcgccg atgatcctct acaccaccga
     4381 agtggaacag gccaaattct ggtaccagta tctgcgcgag cgtggttatc ggcgctgtgg
     4441 attgttccat ggtgcgacgc caatgcagga acgagaaggt cttatccacg cttggcgtaa
     4501 cgatgggctg gatatcatgg tggcgacttc ggcatttggt gttgggatgg ataaaaacaa
     4561 cgtccgttcc gtcttgcatg tcgcagttcc tgaaaattta gatcgtttct accaagagag
     4621 cggtcgtggc ggtcgtgatg gtaaagcatc ggtcgcgcac attattttcc atcacaaaca
     4681 gctacaggtc gcacggaaca ttaaccgtac caaactgatc ggtgccagca aaggatattt
     4741 gcgctgggcc aaaatgcacc agttgcgcga gcagcatcag ccgggtcagt tcatcgttcc
     4801 gctacgcgcg aagcatgctg acattcgcat ggattcgcag agcaatgtgg actggaacct
     4861 gcgtacctta ctgttaatgc agcgggccgg gtttatcgat atcgcctatc cgcctccgga
     4921 tcttagcgct atcgccccgg acgagcgtga tgaagcaaag atccgggcgt ggtttgacca
     4981 ttatttcaac catattcagg tttccgtacg ccgtgatggc catatggatg aagcgcggtg
     5041 gcatgagggc attcaggccc accgttcgca tgaactgggg atgcgaaaac agggatatag
     5101 cgcgttggag gagtggctca atgacccttc aatctcgttg tgccagaagt tagcgcaatt
     5161 ttacaccttg gatggctttg tgccggaaat agcctgcggc ggatgcccag cctgtcggag
     5221 taaagggtat cctcccttta ctccgacgct cggtcgtatc gcgcacgtga cgggcgaggc
     5281 ggtgcggaac gtcatgggaa atgagcaacg cgtttattat cctgcgacgc tgacgaaccg
     5341 gctactgctt cgtcaatgga gtgactggat tgcccgactc ctagccaatc gccagattca
     5401 ggctatccgc gccagccaga gcgtactcgc ccaacttagc gcggtactgc ccgcaggttt
     5461 gcctttctgg tgttcgcttg ccgttgatga agaaaacacc tgctgggacg aactggtgct
     5521 ggtgttgccc gacgaagcga tgccggaact ggacattttc gccaccatca atcgcattat
     5581 tgttgcgccg gaacgcttac aggagccggg ctaccgcggg cgtcgctggt gggaggttga
     5641 taccagtgcc gtggcattag aacaatttca gaggaatatt atctagtggc aattaccaat
     5701 aacgctgccg cagggagcca gatccgtctg ctgtgcatga tcgaccgggt actgaatcgg
     5761 cggaccggac aggcgatccc gatggtagat ttgattgaat tactgcgccc ggagatgctg
     5821 cccgtcagcg atggcgcgag aaaacgtctg cccgccgaag tctctttctg gacaaaagaa
     5881 gggttgtgga aaaatgagag cgctggcctg agtcaacagt cgccgctttc cagcgaacgt
     5941 aatctgcccg cacgggtttt gcgtacgcta attaacagtg agaaaacgac gccgctgctg
     6001 actggaacgc gcggtcagcc cttgctgatg tgtgtcacct ccatgcttgc acaggaaaaa
     6061 tatacgttac gtggaagcac gccgctgaca aaagaggcgg tgccgctggc cgttggcccc
     6121 atgattaata ataaattagc aggtgagggg cgacgtaacc tcaacgtctc taacgaagca
     6181 gaatccttcc tgaattacgc gtattttctc ggttttacag aaccttatct gaatggctgg
     6241 gtgatggacc ctacccgcgc cattgaagga attttggata acctggggct taccgccggg
     6301 atgccaattc aggagttcct cagccaactg gcggagtttc tgccaatgct ggatggggga
     6361 gcctaccgtc agcaggtaga accgatgatc tctgctgaca actggcaacc gctggagaaa
     6421 catatgatta gtgcttcttt gagccaggcg ttgcttcgtc ttgagctgac tatgcagttg
     6481 gttttcagtt cgcgttctga tgacctggac gccatggtcc tgcaaggtcc ggatggcagc
     6541 ctgcgtcgta tcagtaccgt tagccctggg gaggcaagaa aatgagaatg atcgaatact
     6601 ggccttcctt gaaaaacatc aatgaatgta ttcgcaccga ggcggaagag ttggcggagt
     6661 acaccttact ggccgtgcat caaccggtga atttgctgcg tcgtgataaa gacggcaaca
     6721 acctcggata tgcgaaagaa gaggatctgc tcaaccactt tctggaaacg ccgcgcccaa
     6781 tcccggtagt gggtaaagct ggggtgggga aatcgcacat catccgctgg ttggatgcga
     6841 aactgcgatt gcgccaggag tacaaagaca agaagtggca cattgttcgt attcccaaaa
     6901 gtgccagcct gcgtgaagtt ctcacctcaa tgctggctgg cctggaaggc gagatcttcg
     6961 acgaggcgcg ggaagacatc aataaggtgt cggataaacg cactcctcgc gagattgctg
     7021 aatggctact gatgctgatg gggcaagagc tgcgcaacct gcatgaacgc aacaagcagg
     7081 atatggaagc actgaaaaaa cagatggcgg ggagtactcc tgagcagtta aaagccatga
     7141 agcctcaggc gacacggctc cagaaaatcc acatccacgc tgaagataat gcattgccaa
     7201 cgctgattaa cgatgcctac ttcaagcagt ttttgttgaa agaagagcac tgcctctatc
     7261 gtcttgccag ccgtctgacc aacggcgcaa ccagcagcga tctcgacgaa ggggaacagc
     7321 aacttcaggc caaagacctt gatttcagct ttaacatcaa tgacctcagt ctgccggcgc
     7381 gtcagtatat tcagaaaagc cgccttaata cgcatgagga cgggcgcaaa gatgcctccg
     7441 agatcctcaa cctggtgctg ggtaaagcga cgcaggcgct gtttaaccag ctcttcaact
     7501 tccgtggccg cagtttctcc gacctgttta ctcagattcg tcaggcgcta catgaaaagc
     7561 atatgacgct gatggtgctg gtagaggata tgtcgctcat caccgcgatc gaagatgtgc
     7621 tgatcgacag cctggaaagg gaagggatcc gagacgggaa agaggtgctg tgccctgtgt
     7681 gttcagccat tgccgttact gacggttatc aggggtatgc ccgccgccgt caggggatgc
     7741 tggatcgcgc gaagggtgaa tgggtgatcg aagaggtagg gagcgggcgc gaagagacgc
     7801 gactgcgtat tgtcgatttt tgtggtcgat acatcaacgc ggcccgcttt gggagcgaag
     7861 ccttattaca gcgctgggag caagccgccg ataaatctca ctggccacct gactgggaag
     7921 ccagcgcaaa cggtgacgaa atggccgaag tgtttgggcg aagcgagcaa ggtttttctt
     7981 tgtatccctt caacgctagc gctatccatg cgttggcaga ggcattctgc cgcgacgatc
     8041 gcaacgaatt aaaattcaac ccacgtcaga tcatcaacca gatactgttg cgtgtactac
     8101 agcactgtcg ccgtgatgcc gatgagggac gctttccacc tccgcgtctt ggcgatattg
     8161 ctgctccggc agggcttcga agctggctgt tccgccaggg ctttgccgat accgagcgtg
     8221 ccgaatccgt ggtcgcgctg tggggttatc ccgccgattc cgacgccact ttggccagta
     8281 cgctgccgcc tgacgttgcg cgcagtttcg atctggaaga tctggcgcag gtgctggaaa
     8341 acacaaaaag tgccccgcta gttgaacaat cggtgatgcc aacgcgggtg accaaagttg
     8401 agcagcaggt taaaaaaacc gtgcagccac ctaaaccagt gaaacccgtg ctcaaggagg
     8461 ataaagatac cttggctatc cgcgcgctgg atgcagcggt gggcgactgg atgctaaaag
     8521 gcgcgccgct tgagatcgat cctgcacgat atatcaaaag ctccctggcc tttttctttg
     8581 ataagcgcgc cgtggcggaa tgggcgggca gcagctatcg gccgaccctt tggttgggta
     8641 aatccaactt tgttgccgtt gagttaccta acgcgcaggg taaccgtggc gtacatgtgg
     8701 tgaaatttat ttcccagtcc gaatacgaca agcgctcggt gcaattaacg gatgccgcga
     8761 tggcgctggc gcggtttggc tactacagag aaaactcctt aactaagacc gaggactgga
     8821 gctacccgga gggcaaaacg gattacctga ttattcagag cttctgcgat cgctgggtta
     8881 actatgcgct gacggaactc gttaagcata agcgcaacga tctgccactg cttctcagcg
     8941 agcagattgc acttgccgat gcgctggggg tgattaaaac ggccgatggt ccgaaagagg
     9001 tgcttgggcg actgctgcaa aacagcaaaa cgctttcttc acagttccgc accgggatca
     9061 cgaaggcaat taccgaactg cgaggagagg cgttagcaaa gtgggatgat gcccaggacg
     9121 catggctcag cctggtggcg ctaaacgatc atgcgctgga aggcgatctc ctgctgagtg
     9181 cgatacaaaa ggtgttaaaa aaacgctcaa ataacacaca cgcagccgtg gtgaaaaagt
     9241 cgctcagtga aatccgcccc gcactggata ctgctgctct gtttgccgac tgtgaaaatg
     9301 cggatgattt ttccgaactc gtcaccggcc tgactcagtt ggttaaatcc cttggggata
     9361 gcggtgatta tcctgcggat atgacacccg acagtagtac gcttgcgaac tctctgactg
     9421 ggttaacgga aggtggagtc tggttgacga tcctcaagtt gcgaggcatt acgcaatctg
     9481 aagatccgct tcgccaatgg cagttacttt gcgaactgga cggtacttta attaaccgac
     9541 tcatattcac aatcagtggc tggcagcagg tcaacaagcg ggtgctagcg aatattaccg
     9601 cctacaacca ttcgcatggc agtcaccaga tcagcgaatt tcgcacgcag attgaaagca
     9661 cgttgcagga acttcatcag gtcctcgacg ccatgcagtc cgtcgcaggg gaacagtatg
     9721 acaacgctta agacccaact tacgcaactg cgggagcagt atcagataaa acagcaggtt
     9781 cagtcgctgg aagaggtcta cgatcggtac cgcaccattc gtgacggctt gctggatgtt
     9841 gccgaaccgc tgcaaaaagc ccaaaagcaa cttgaagtga tcggaacgct accggaacca
     9901 cactccgcta tcgatttttc gcaagaggcg gcgcttttcg gcggtgccaa aacgcagttg
     9961 gatgcgctga cggcgcgctt caaagaaagt ggcgataaaa ccgagcagtg cgggttttgg
    10021 gaaacggtgc gggttctgaa gagcgtacat gaaagtattg atgataaggt ggatgcaacc
    10081 tggaaagcgt ttgttgccga tcttgaagcg cgcgcaacgc tggatcccgt ctttctggaa
    10141 caacagcgga cgctggggaa cgtgttggtg tatgacgatt atcgcaaagc gcgcgataac
    10201 tttaatcggc agaagttaag cgggccagac agcctgtcgg tggtaaaaaa cctgcagaag
    10261 tatagcgatg agatgatcgc gcttaaacgt tcaatggacc tggatgcgcc agacgatgtt
    10321 ctgcgctttt ttgaggcgct ggacagacat aatcatgcct cgcttacgct gctgacgccg
    10381 ggcgtgatta cctggctgga agaaaaagat atgctgccag cgtttaacgt gacacgtaag
    10441 agaatgattt aatgaaggga atcgatcttt cgcttttgca acgcgtgcta gatgcactgg
    10501 aagaacaaga aaatgcgctc ctggtatggg gtgacacggg cggctttttt aatctggatg
    10561 aacttttaag cgttatcgga cgggttttac ctgacgaaga tgccgaagtg gttctggatc
    10621 aaatgctaga ggccgctatg ctgatccagg tgccgcacgt cagtggcacg cctgtctggc
    10681 gaacccgaat gggggagacg gtccatctca tgcgcaacct gcgccagtgg atgcataagc
    10741 aaaaactgga ccagagcaaa acgctggtat cggactatcg ctttattcgc cggccgcgca
    10801 gctatcccga ccgcgtttac gaaccggcaa cgctgattag cggctggaaa aaacagctga
    10861 aattaagcga tcgcgtttgt gacatcatgc gccaggcgct ggcgtcagat aagcccttcc
    10921 ttctggcggg ttttcaggtg cgcgctaccg aacgcattat gcagtgctgg caggatcatc
    10981 aggtgaaaag caacacggcc agcgggacca ttgtctgtgc tggcaccggt agcggtaaaa
    11041 cgctgtcgtt ttacctgcct gcgctaaccc gtctcgctga ggatatttgc aacaaccctg
    11101 agcgcaaggt gcgcattctc gccatctatc cgcgaaaaga gttgctcaag gaccagtttg
    11161 ccgaaacttt ttcacagtgc cgcaagcttg acgactatat gctcacggcg gccggaagaa
    11221 aaattcgtat cggtgccttt tttggcgata caccggtgaa ggcggaatgg tctaaggaag
    11281 acgttaaggg gaaggttggt ctgccgttcg ggttgatgaa atgccagcag ccccacccgc
    11341 atcatcctaa gcagaggtgc ggaggggctc tcatttggcg tcgtgaggac gtatttggcg
    11401 agcgtgaggt acttacctgt acgcagtgcc agcatcaggt ggatcagagt gaaatcatga
    11461 tcacccgtga tgcgcagcaa catcgcggcg acgcgccgga cattctgttt accacaacgg
    11521 aaatgttgaa cctccagatg aactccacct ggagcaacca cctgtttggc gtcggcgagg
    11581 gctatgggcc gacgctggtt ctgctcgatg aggtccacac ctacagcgga accaccggcg
    11641 cgcaaactgc gtttctgctg cggcgctgga tgcagcgcac cgattgtttg ccgcattttg
    11701 ttggcctgtc tgcaacgctt accgacgcgc agcacttctt tgccaggttg gtgggtgccg
    11761 ctgaggagca ggttgcgctg atccagccct acatggaaga tatgatcgaa gagggggccg
    11821 agtacctgct ggcgctgcgc ggcgatccgg tttctgaaac ggcattactc tccaccacca
    11881 tccaggccag tatgctaatg gcgcgcatgc tggatagcaa agcgaataag tcgcaaggta
    11941 cgtggggcaa gaagaccttc atcttcaccg acactctcga cggtaacaac cgtttgtacc
    12001 atgacctcag cgatgccgaa ggttgggaga cgggcccagg gcactcgcgc attgatcact
    12061 caccgttggc tgtgttacgt agcccttttg ataatactgc acctgagcgg tctaaaaccg
    12121 aactggggca aaactggagg gcggccacgg atattggcca cgacctcgcg gaaaacaaag
    12181 tcatttcccg aacgtcgagc caggatgccg gcgttgatgc atcagcggat gtcgtggtcg
    12241 ccacctcttc gctcgaggtg gggtataacg atccgctggt cggtgccgtt cttcagcata
    12301 aggcgccgaa cgatgtcgcc tcctatttgc agcgtaaagg tcgtgccgga cgtccacgag
    12361 gcatgcgtcc ctggatgctg gtggtgctct ccgagtttgg ccgcgatcgc gtcgagttcc
    12421 agcgttatga agggctaatg agcccggaga tcaaacgtca gggtctgccg ctggacaacc
    12481 agcacgtaca gaaaatgcag gcagcaatgg caaccctcga ctggatcagc aaagtcggtc
    12541 agttcaaaga tctctggagc atgttgaaaa aagctgagca taatcagctg aaatacaatc
    12601 gaatgtatgg cccgcttatc aaactgattg aagaggtgct gagcggcggc aggcggttga
    12661 acgaattgat gcgctacctt caggatgctc tgcaattatc tgatggcgcg gtgcagaata
    12721 tcctctggag cccgccgcgc tcgatcatgt ttgagtttct accaaccata ctgcgtaacc
    12781 tcagaacgcg ctggtcggtt aacggcgtgg agtgggccgg actgcgtccg aatcagccaa
    12841 atagcgaggg cgaacagcac cggagcaatt cacccgtccc ggaatacatc ccgcagaacc
    12901 tgtttagcga gctaaacttg ccggaactgg acattcgcct gaagcgtgga tttgacgatg
    12961 aagaccactg ggaaactctc tctttctggc agggaatacg tgaatttgcg cctgggcgac
    13021 tctctaaacg ctatgccgtc aaatcgaaca aaagtaccga ctggctggtg ccacaatcct
    13081 acgagccgat ggcaggggaa gggcggcaat ttgttgattt ccagataagc gatgcgttcg
    13141 gcgatagctg gcaaaacgag tatgaagttg actatatggg aaaaaccatc aaggttgtta
    13201 aaccgtcgaa ggtcatgacc actcgggccg acattcgccg gatcaatgat aaaagtaatg
    13261 cgcaactgca atgggtgttc aacgtcatta accccgctat tgctacgcca gacgaggtgc
    13321 cgaagggacc ctggaagcac acgctgagcg atgtcacgtt ctacaaccac cagcatatga
    13381 ccccgctgga actggtccgc ttttctactg gatcgcaggc gtcgctccgg ttcaggaata
    13441 aggaacgggc tcacgttgat ttcacctggg ttaatggcga ggaacaggtt ggcgtaggaa
    13501 gtcgtcaatg ggtggatgca atgcgcctgc gttttaacct cacctgtgat gatgtgcttg
    13561 gtctgctaca tcaagaagaa atccagcgag gcatgcgccc cgtttatttt caacatcttg
    13621 taagacagtc gccggaattt gaatttgaca gcttcaacgc ggactgggcc attgagtgtt
    13681 ttatggctca gcttgcggag acactggcaa acggcgctca cgcctcggtt gagtcggcat
    13741 tacgagaaat ggcctccgaa aaagggggag aaaggctggc agatattccg gccagcctgt
    13801 tccagccgga taccgacaat gaaacaggca ctgatcaggc gctacagatt ggcctcaaca
    13861 aacttcttca gcgaccggaa atacagcagt tgctgctgaa ctgcgcgcaa gcgctctgga
    13921 aacccctgga tgaaatcgac ggttttgttg aatgggcacg ccaggtatta gcggacaccc
    13981 tggctgcggg tgtgcagcaa acgctaagca cgctgcttcc ggacgttgat gagcgagcgg
    14041 tagtgacgga ttccagttgg atgagcgatc cccgcaaggg agcagaatgg ctggaaatct
    14101 ggctctgtga gatggagtct ggcggcagcg gtattctgat tcgtttacag cagaagtggg
    14161 ctgaagatcc tgtctccttc ctcaatgtac tagtgcgtaa tctttcagcc agcgactacg
    14221 agcagatcga ttacgatctg cgaactgtgt tgcagatgat gcaaacggat gacacgcttc
    14281 gtatggcgat ctcagccgta cgtgaagcca gcaacatgga cgcccggcgg gaggctaaca
    14341 agaatctgca tttgcagctt tcacagcgcg gtatgcgcct ttcccacagc tttaccacgg
    14401 tgctgtactc gcggattctg cgggcgggca gcggcgagca cacagatacc cagctttatc
    14461 agctactgtc ggactggtcg tcgcttgaag cgagcgcggg tattgaattc agtatgaaca
    14521 ccatggcgca cgcgttggcg gtgaactcgc ttggcgtaga aactgatgct tccgtcgtgt
    14581 ttgacgagca gtgccggaat caaaacctgc tctggccacg aggctatacg atccgacagg
    14641 ctgagctcgg cttttacaat atgttttgct cgcgcaaagt cacaaccgag cgtctgcttg
    14701 ctggcgcgtt atttagcgag caaatcgaaa agatcgccct tgacgatgac tggcttgggc
    14761 atttgcatgc ggcgctgcgt aaaggcggtc gtgcggaact gatcctcact cgccagcagc
    14821 gaaatcaatt acatcaggtc attaccaccg tgcaaattga acccgtcgat catttgggac
    14881 tgctgctgta tccgcgtctc ggtgaagtcc gccgtgagca ggatgtgctc atattgcgta
    14941 ttgaactggc agaggcgatg caatgatcgg acgtgcaaaa cagcggcaga tttttttaca
    15001 tggctccgca gggcaccggc aggtgcgaga agtcctcagc gcccaggttg ttgctctgat
    15061 tatgcagcca gagccaatct ggctggtttc gccgtgggtg agtaacttct cactactgga
    15121 caatcgctca ggcaattggg acagcattga tcccgcgtgg ggaggccggg aggtggattt
    15181 cattgagctg ttagcctgtg cggtgaataa cggcgcgccg ctttcgctgg tcacccgcga
    15241 cgaaccgatg aaccgtatct ttgtaaaagc gctggaaaaa cgcctgagta actttgcgac
    15301 ctttcgtttg gaatggcgta accagttgca tacaaaaggt tttctgacgc caaatttttt
    15361 tctcaagggc tcgatgaatt ttacccggag cggtgttaat cgaaatgaag aatttgtcga
    15421 actgaactgc gattcgcata tgatcggtga agcgatggcc gaatttgaaa gtcagtatct
    15481 ttttattgga tcaaaggaag cgttctgatg tgtgcagact ggctgaaaaa ttattacgct
    15541 gaagataact atctggatta tgacaaggcg atgcaggggg ggtatggaat cccacaaatg
    15601 aatacgctga ttcaacaggc cgcagcgcta cggatgccct gtatcgttcc ttcaacgcga
    15661 acgggaagaa cggtgtttta tgctctggct gagaatgcga aaagcctaga cgaattacga
    15721 cgtattttaa ccgcggcgtt gggttccgct gacactactc cggatattaa atcactcaat
    15781 cattctgacg atggcgggga gcagttgctg ctggagaaat ccccggacgg atttctggcc
    15841 ttcgatttcc taccggtgcc ggacggttcg ccgcaacagg taaaagaatg gcaggcagca
    15901 cgaatgaaac gggtttatgc catgctgcaa acggtgatgg atctttatca tcagcgacct
    15961 gtcttacatt cgctggtcag tcggcaaacc ggacgaattc tcagggattt ctacacagca
    16021 tgccaggctc gcgatggcaa gattgccgaa cagtatctgg aagagttacg cggaaatcag
    16081 acgctttctt ctctgaatct cctgtttctt gagctacagg ggatggcggc ttcagcaaaa
    16141 tgggacgcga ttctcaacca ccctcggctg gaagtattat tgcgcggccg ggtgccggag
    16201 cgtatccagc gcctgctgtt gcgatcctct ggccatttaa tgcttaacgc catccgcgat
    16261 gcgcacttcc cactggacag gcgtgaagat gccaggcgac tggtgctggg cttattgccg
    16321 ctgtacaagc ataagccccg ctttgctcat caggcgagtt tcctgcctga ctggcaactg
    16381 tgggccatgg gggccgcgct gctggggatc gacgaatggc aaacggcgac gcctctatta
    16441 gctcctgact ggatacagca ggtcgagggg tgggcgacag gcacaacctc gctgcccgcg
    16501 ccggtagcag cggaagaaca ggcgctgatc caggcgccgg ttatcatgct gataagcctt
    16561 gaaaacgcga ccgatttatt actcgaagcc cttctggcgg acgctgagcg cgaaagtgag
    16621 atctacgcgc aactggcggc gatgcctgaa gcaacacgac aggcattgca aaaaataccc
    16681 aaactctggg aggcatggca ggcgctgaaa aaacgctgtg agcctcagga ctatggctgg
    16741 tgccgctggc ttgcagattt acaacagact acggaatccg aacggtttga atcattgcgc
    16801 cagcaggcga ccgttcacta catggactgg gcaccgtcaa ccttcagcga aacgcagtgg
    16861 caggcgctgc ttgagcatca gtccaacacc cagttgagta aggttttgcg agacgtactg
    16921 ccaactcttc tgaactggtt ggaagaatat gacgttaagg tatctgcctc cttgtggcca
    16981 gactggctta tgctgctggc agttgaggac atacgctcag aagaagatgt acgtctcggc
    17041 gggatgattc tggataaatt cctttccagt ccctttacct gcgaagaata ttcggcagcg
    17101 attgaatcga cagagattct atgtgctgaa aacgtgtcgg tgaggacgtt gggttattct
    17161 cttgatattg ctgaacttct gtatgacagg gtcactgcaa acgaagcctc cagattaggt
    17221 ttctggatta agttgcagga gttactcaag catcgctggg agcgcctgga tgccagtatg
    17281 cagttttctg cacgcatggt ggaaaggctc tatctcggcg aacatgccgg gaacgttttc
    17341 ccgtcggagg acaatacgcc gggcgtagca tcttcgcttc atcgcgatct cagcggtaaa
    17401 accttagcga tttacagcct gatgaaaggg gcggcaagac gcggaaaaga ggcgttgctt
    17461 cagctttatc caggactgga agtagagctc aaccacgatc atgtcgctac ccctgcgctg
    17521 gtgaatctgg cgaaaacagc ggactacttt atcttcgcta gtagcagttc aaagcatcag
    17581 gcgttctatg ctgtgacgga ttatcgcaaa gagatcatct acccctctgg aaagggggcg
    17641 tccagtatga tcgcatcttt cgtcagggcc atggagtaaa taaggaatag cctctgctat
    17701 tccttatttg ttgtcacctc tgaaccacct cctatggagg tggtgattgt tcctgtaggg
    17761 ctataacccg aagaaagccc atgcggaaat gtttcacctt atacccggat gcttcagaga
    17821 agcagtgcgg tttttctcgg taatgcaacc agaaacaatc ctgaagaacg ggtaataaat
    17881 gttttaagtc tccttttagg ggaccgcatc aaagccacct tctaagaagg tggattcttt
    17941 actattcacc acggtaaatg cagcctgcgg agcatgtctc tttcactatc accgcgctca
    18001 ggatagatag ctgaggctta atctgattcc aaatccattc agcaagcact tcgctggtag
    18061 ggttctccag tcccgttata tcattgagat agtagtgatc cagtctatcc agagtgggtt
    18121 taaaaatcgc ttttagttcg gcaaagtcga taatccaacc ggtatgctta tccacttcac
    18181 ctgttatctc aaggcgcacg acaaaagagt gtccgtgtaa gcgtccgcat ttatggccat
    18241 tcggcacatt gggaagacgg tgcgcggctt caaaggtgaa atctttgtat aacgttgttt
    18301 tcatagggac ctcatttttt gttataccgt aacaataagg ggcgatagtt cctagcattt
    18361 atgagttaac ttttaacatt ctattgggta ggcatgaata tttaggtcga gtttcaacta
    18421 gactgttttt tactgtggga cgtcatgtag gatgagcata cacctaaatt gtccgttatc
    18481 gctggtatca gttgcacaac gaccacctca ctgacatcga cgaagattgg tgacaagaaa
    18541 gcatttcagc aacctcatga tcccatcttt gttagctggc tgaagtctcc ggtacggcag
    18601 gcagagcaat agcattcacc atagtaattt ttcccgcatt gtatacagtt ccagtcggct
    18661 ttatataccc ttggtgttgc atcgggaagg aagcgttgca ggcgtcttgc gaggtgtatt
    18721 tcactcgcgt ctggctttac gtaggggcaa ttgtgctgac cattttcggc ggaacccgca
    18781 tcaatgtgtt caaaccacgg gcgttcagtg tcgtattcag gatggtattt cagtgcactg
    18841 tgacagaggt ggcaggtata gcggtcgtaa ggggcggtct gtgccgttct ggcaccggta
    18901 agacgatcgt taccgtcgag agcgataaaa gatttagcaa acataatatt actcccgcca
    18961 gacaacacgc aggcgtaagc cgtccctgaa aggcggacag acgccatgtg gttgaatggt
    19021 ttaaaaggat tgagaaggga attgctgtgt ggttgtcaca ctatgggatg ttggcgtaaa
    19081 taaatcgcca ccagcaaaac ggatgtagag aatatcagcc ctggctgcag ccgaatgagc
    19141 gttcggtact gctgtatcgc ttggtgcgat gctttgccat atagctcaaa cggaaagtca
    19201 gtgcatccag tttatccatg aaacccggct tactggatca aaatttacca ttgcgaaagc
    19261 ttgttctgga aaagctggag atgaaatggt ctccagagca aatatcagga tggttaaggc
    19321 gaacaaaacc acgtca
//
LOCUS       DAPHBJ010000078        19285 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.078, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000078 DAPHBJ010000000
VERSION     DAPHBJ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.078"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(102..749)
                     /gene="ychE"
                     /locus_tag="RHZ76_002424"
     CDS             complement(102..749)
                     /gene="ychE"
                     /locus_tag="RHZ76_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HDV2813052.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1226..3901
                     /gene="adhE"
                     /locus_tag="RHZ76_002425"
                     /gene_synonym="adhC"
     CDS             1226..3901
                     /gene="adhE"
                     /locus_tag="RHZ76_002425"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDV2813053.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIRETGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            4025..4921
                     /locus_tag="RHZ76_002426"
                     /pseudo
     CDS             4025..4921
                     /locus_tag="RHZ76_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(5203..5820)
                     /gene="tdk"
                     /locus_tag="RHZ76_002427"
     CDS             complement(5203..5820)
                     /gene="tdk"
                     /locus_tag="RHZ76_002427"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDV2813054.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVDSLTAIQERHRHD"
     gene            6425..6838
                     /gene="hns"
                     /locus_tag="RHZ76_002428"
     CDS             6425..6838
                     /gene="hns"
                     /locus_tag="RHZ76_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HDV2813055.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(6982..7890)
                     /gene="galU"
                     /locus_tag="RHZ76_002429"
     CDS             complement(6982..7890)
                     /gene="galU"
                     /locus_tag="RHZ76_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDV2813056.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(8092..9105)
                     /gene="rssB"
                     /locus_tag="RHZ76_002430"
     CDS             complement(8092..9105)
                     /gene="rssB"
                     /locus_tag="RHZ76_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HDV2813057.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(9197..10102)
                     /gene="rssA"
                     /locus_tag="RHZ76_002431"
     CDS             complement(9197..10102)
                     /gene="rssA"
                     /locus_tag="RHZ76_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HDV2813058.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            10215..10673
                     /locus_tag="RHZ76_002432"
     CDS             10215..10673
                     /locus_tag="RHZ76_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HDV2813059.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            10723..11565
                     /gene="purU"
                     /locus_tag="RHZ76_002433"
     CDS             10723..11565
                     /gene="purU"
                     /locus_tag="RHZ76_002433"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HDV2813060.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            11725..11809
                     /locus_tag="RHZ76_002434"
     tRNA            11725..11809
                     /locus_tag="RHZ76_002434"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11759..11761,aa:Tyr,seq:gta)
     gene            11853..11984
                     /locus_tag="RHZ76_002435"
     ncRNA           11853..11984
                     /ncRNA_class="other"
                     /locus_tag="RHZ76_002435"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            12019..12103
                     /locus_tag="RHZ76_002436"
     tRNA            12019..12103
                     /locus_tag="RHZ76_002436"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12053..12055,aa:Tyr,seq:gta)
     gene            12150..12281
                     /locus_tag="RHZ76_002437"
     ncRNA           12150..12281
                     /ncRNA_class="other"
                     /locus_tag="RHZ76_002437"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(12465..13142)
                     /gene="narI"
                     /locus_tag="RHZ76_002438"
     CDS             complement(12465..13142)
                     /gene="narI"
                     /locus_tag="RHZ76_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDV2813061.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGSASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(13142..13852)
                     /gene="narJ"
                     /locus_tag="RHZ76_002439"
     CDS             complement(13142..13852)
                     /gene="narJ"
                     /locus_tag="RHZ76_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDV2813062.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(13849..15387)
                     /gene="narH"
                     /locus_tag="RHZ76_002440"
     CDS             complement(13849..15387)
                     /gene="narH"
                     /locus_tag="RHZ76_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDV2813063.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(15384..19127)
                     /locus_tag="RHZ76_002441"
     CDS             complement(15384..19127)
                     /locus_tag="RHZ76_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HDV2813064.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         4866 a         5065 c         4623 g         4731 t
ORIGIN      
        1 atttttacct attccttttg cttttgataa ctgcgcggga acaaagtaaa aataatgacg
       61 aagtaaacgg agcatggcag ctccgtttca tttgaaagga attaattaag caggccgggg
      121 aaaatcccct taataccagt aacgataaat tcaatcccca atgccatcag caatagcccc
      181 ataatacgcg taatcacgtt gatgccggtc tggcgtaaaa cccgtaccag ccacggtgcc
      241 atgcggaaca atccccagca acataaagcg aacaatgcaa tagccacaaa gaaaccaaac
      301 agatagctaa tgctgtgata acgcgtaccc cagacgatgg tagaactgat cgcccctggc
      361 cccgccatca acggcaacgc caatggcacc acaccaatgc tttcacgtac cgcggtttct
      421 gatttttctt gcttgttctg tttatcctcg ccaagcttgc cgctgatcat cgacatcgct
      481 atcgtcacca ccaggatgcc cccggcgata cggaacgaat caattgatat accaaaaagt
      541 tgcagaatcg tgtcgccgag aaaaagcgag atccacaaga taatggccac agacaggttg
      601 gctgtaaggt tagttttgtt tcgcgctgcc gctgtctgat aactggtcat gctgataaag
      661 acgggaataa tccctaccgg gttgaccagc gcaaataacc cgatgaaaaa tttgaagtaa
      721 acgggaaaat caaaaaaggt ctgaatcacg gttagctccg aagcaaaagc cggataatgt
      781 tagccataaa taaggttgaa aagacgcgct gacaatacgc cttttgacag catttttcac
      841 ctcctaacta cttaaaattg ctatcattcg ttattgttat ctagttgtgc aaaacatgct
      901 aatgtagcca ccaaatcata ctacaattta ttaactgtta gctataatgg cgaaaagcga
      961 tgctgaaagg tgtcagcttt gcaaaaattt gatttggatc acgtaatcag tacccagaag
     1021 tgagtaatct tgcttacgcc acctggaagt gacgcattag agataataac tctaatgttt
     1081 aaactctttt agtaaatcac agtgagtgtg agcgcgagta agcttttgat tttcataggt
     1141 taagcaaatc atcaccgcac tgactatact ctcgtattcg agcagatgat ttactaaaaa
     1201 agtttaacat tatcaggaga gcattatggc tgttactaat gtcgctgaac ttaacgcact
     1261 cgtagagcgt gtaaaaaaag cccagcgtga atatgccagt ttcactcaag agcaagtaga
     1321 caaaatcttc cgcgccgccg ctctggctgc tgcagatgct cgaatcccac tcgcgaaaat
     1381 ggccgttgcc gaatccggca tgggtatcgt cgaagataaa gtgatcaaaa accactttgc
     1441 ttctgaatat atctacaacg cctataaaga tgaaaaaacc tgtggtgttc tgtctgaaga
     1501 cgacactttt ggtaccatca ctatcgctga accaatcggt attatttgcg gtatcgttcc
     1561 gaccactaac ccgacttcaa ctgctatctt caaatcgctg atcagtctga agacccgtaa
     1621 cgccattatc ttctccccgc acccgcgtgc aaaagatgcc accaacaaag cggctgatat
     1681 cgttctgcag gctgctatcg ctgccggtgc tccgaaagat ctgatcggct ggatcgatca
     1741 accttctgtt gaactgtcta acgcactgat gcaccaccca gacatcaacc tgatcctcgc
     1801 gactggtggt ccgggcatgg ttaaagccgc atacagctcc ggtaaaccag ctatcggtgt
     1861 aggcgcgggc aacactccag ttgttatcga tgaaactgct gatatcaaac gtgcagttgc
     1921 atctgtactg atgtccaaaa ccttcgacaa cggcgtaatc tgtgcttctg aacagtctgt
     1981 tgttgttgtt gactctgttt atgacgctgt acgtgaacgt tttgcaaccc acggcggcta
     2041 tctgttgcag ggtaaagagc tgaaagctgt tcaggatgtt atcctgaaaa acggtgcgct
     2101 gaacgcggct atcgttggtc agccagccta taaaattgct gaactggcag gcttctctgt
     2161 accagaaaac accaagattc tgatcggtga agtgaccgtt gttgatgaaa gcgaaccgtt
     2221 cgcacatgaa aaactgtccc cgactctggc aatgtaccgc gctaaagatt tcgaagacgc
     2281 ggtagaaaaa gcagagaaac tggttgctat gggcggtatc ggtcatacct cttgcctgta
     2341 cactgaccag gataaccaac cggctcgcgt ttcttacttc ggtcagaaaa tgaaaacggc
     2401 gcgtatcctg attaacaccc cagcgtctca gggtggtatc ggtgacctgt ataacttcaa
     2461 actcgcacct tccctgactc tgggttgtgg ttcttggggt ggtaactcca tctctgaaaa
     2521 cgttggtccg aaacacctga tcaacaagaa aaccgttgct aagcgagctg aaaacatgtt
     2581 gtggcacaaa cttccgaaat ctatctactt ccgccgtggc tccctgccaa tcgcgctgga
     2641 tgaagtgatt actgatggcc acaaacgtgc gctcatcgtg actgaccgct tcctgttcaa
     2701 caatggttat gctgatcaga tcacttccgt actgaaagca gcaggcgttg aaactgaagt
     2761 cttcttcgaa gtagaagcgg acccgaccct gagcatcgtt cgtaaaggtg cagaactggc
     2821 aaactccttc aaaccagacg tgattatcgc gctgggtggt ggttccccga tggacgccgc
     2881 gaagatcatg tgggttatgt acgaacatcc ggaaactcac ttcgaagagc tggcgctgcg
     2941 ctttatggat atccgtaaac gtatctacaa gttcccgaaa atgggcgtga aagcgaaaat
     3001 gatcgctgtc accaccactt ctggtacagg ttctgaagtc actccgtttg cggttgtaac
     3061 tgacgacgct actggtcaga aatatccgct ggcagactat gcgctgactc cggatatggc
     3121 gattgtcgac gccaacctgg ttatggacat gccgaagtcc ctgtgtgctt tcggtggtct
     3181 ggacgcagta actcacgcca tggaagctta tgtttctgta ctggcatctg agttctctga
     3241 tggtcaggct ctgcaggcac tgaaactgct gaaagaatat ctgccagcgt cctaccacga
     3301 agggtctaaa aatccggtag cgcgtgaacg tgttcacagt gcagcgacta tcgcgggtat
     3361 cgcgtttgcg aacgccttcc tgggtgtatg tcactcaatg gcgcacaaac tgggttccca
     3421 gttccatatt ccgcacggtc tggcaaacgc cctgctgatt tgtaacgtta ttcgctacaa
     3481 tgcgaacgac aacccgacca agcagactgc attcagccag tatgaccgtc cgcaggctcg
     3541 ccgtcgttat gctgaaattg ccgaccactt gggtctgagc gcaccgggcg accgtactgc
     3601 tgctaagatc gagaaactgc tggcatggct ggaaacgctg aaagctgaac tgggtattcc
     3661 gaaatctatc cgtgaaactg gcgttcagga agcagacttc ctggcgaacg tggataaact
     3721 gtctgaagat gcattcgatg accagtgcac cggcgctaac ccgcgttacc cgctgatctc
     3781 cgagctgaaa cagattctgc tggataccta ctacggtcgt gattatgtag aaggtgaaac
     3841 tgcagcgaag aaagaagctg ctccggctaa agctgagaaa aaagcgaaaa aatccgctta
     3901 atcagtagcg ctgtctggca acataaacgg ccccttctgg gcaatgccga tcagttaagg
     3961 attagttgac cgatccttaa actgaggcac tataacggct tccacaacag ggagccgttt
     4021 tcttatgcca cttctcaatg atctgctcga tttcagtgac catccgctta tgcctccgcc
     4081 ctctgcacaa ctatttgcag aacaccttcc caccgagtgg atacaacact gcctgacgct
     4141 ttctgctcat gcgaccgttc gccgccgtcg tttaccgggg gacatggtta tctggatggt
     4201 ggtgcaatga gccaattacc gatgttgttc gccgtctgaa cctgagcgcg gatggcgaag
     4261 cggggatgaa cctgctggcc cgcagcgctg tcacccaggc gcgtcagcgc gtgggggccg
     4321 ccccagtgga atggctcttc cgccagaccg cacagacagg ggcgcggaac gttacctgaa
     4381 ggatgactgg cacggcctgc aactttttgc cattgatggc gcacagttca ggacacctga
     4441 taaacccgag ctgcgtgaat attatgggtc tgccaacaca tccactaagc ggcagaacgc
     4501 ctacccggtt atgcgtctgg tggccttaat gaacctggga agtcacattc tgctgaatgc
     4561 cgtgaccgca ccttaccggc agagcgaaac tgtgctggcc cactccatgc tcgccaccat
     4621 cccggataac tctattacgt tgtttgataa acttttttac agcgaagacc tgctgctgac
     4681 gctgaaccag aagggttgta accgccactg gctactgcct gcgtggaaga atatcgcatc
     4741 agaaatgata gagctgggaa acacggcgtc accagggacc atcccgaaac gactggagca
     4801 tcttcggggt gctctggaag tggtgttcat aacaaaacgc ccccgaccat caaggccgag
     4861 gtcggttaag atatcaaaaa cccgttatcc tgtgaaacat agtgccgctc ctcttaagtg
     4921 aacggcattg cccttctggg gccgtatttt ttgtttaccc aaagcaactt ttccataaac
     4981 cgacagcatt agccttcatc atatttgcga cgatgtataa cgcctaaaca cagggatatt
     5041 gtactttaca ggtcacaagt caacgtcggt gcttaagagc cctgtgaggc gtatagcggc
     5101 gttaaaaaac tgccgagaag ggtatatagc ccggaagaag tgcgtaaaac gaactgacag
     5161 gataaaagtg ccctgctcac cctgtcagta aagaaattct tattaatcgt ggcgatgcct
     5221 ttcctgaata gccgttaatg agtcgacttg taacgcctct ttatagtgtt tacggcatac
     5281 agaaacgtat cgttcattac caccaattac cacctgctca ccttcgttat aaggtctgcc
     5341 tgcttgatca agacgcagca ccatgcttgc tttacggcca caaaaacaga tggtttttaa
     5401 ttcaaccagt ttgtcggacc atgccagtaa gtattggctg ccaataaata attcacctcg
     5461 aaaatcggta cgtaaaccat aacaaagtac gggtatatcg agttgatcga caacctccga
     5521 taattcatat acttgttgtc tggttaaaaa ctggcattca tcaaccagta cgcaatgaat
     5581 tgcctgctgt tcatgttccg cacgaatctc atcaaataat gatgaatttt ggttaaataa
     5641 ttttgcaggc gatgacaaac ctatacgcga actgactttc ccggcaccaa agcgatcatc
     5701 aatttctgcc gtatatacga cagtgcgcat gccgcgttcc tggtaattgt atgaagattg
     5761 caacaatgct gtagacttac ccgcattcat tgcggaatag tagaaatata gctgtgccat
     5821 cagccacaga ccctcaatga tatgaaataa agttggctgg agtttatcat aattcgtcag
     5881 tttttcagct caacagtatg cacagaagtg ggtatatgcg ttctccctta cgaagccttg
     5941 cataatcctt ctgagctatc attacaactg cctcgcttgt tataagcggg taaatgactg
     6001 ctggtaacta ttcacaatct ttaacctgtt gcgcaagtaa tagccctctg ttgacctcca
     6061 ggagatagtg caatactaag tccatgctct tattgcgact gttctacttt tcatcattcg
     6121 cttaataggg aattctcgta aacacaacta atacagaaga ctgaaaggtc gtcagcctac
     6181 gataatctcc ccataaaatg tgacatgaat caggaagttt taacctcacg tgctgcgaaa
     6241 tcatcggtgt aaatagggct atatgccgcg tcttttctgg ctaattttat gaaaagatat
     6301 ttattggcgg cacaaaataa agaacaattt tgaattcctt acattcctgg ctattgcaca
     6361 actgaattta aggctctatt attacctcaa caaaccaccc caatataagt ttgagattac
     6421 tacaatgagc gaagcactta aaattctgaa caacatccgt actcttcgtg cgcaggcaag
     6481 agaatgtaca cttgaaacgc tggaagaaat gctggaaaaa ttagaagttg ttgttaacga
     6541 acgtcgcgaa gaagaaagcg cggctgctgc tgaagttgaa gagcgcactc gtaaactgca
     6601 gcaatatcgc gaaatgctga tcgctgacgg tattgacccg aacgaactgc tgaatagcct
     6661 tgctgccgtt aaatctggca ccaaagctaa acgtgctcag cgtccggcaa aatatagcta
     6721 cgttgacgaa aacggcgaaa ctaaaacctg gactggccaa ggccgtactc cagctgtaat
     6781 caaaaaagca atggatgagc aaggtaaatc cctcgacgat ttcctgatca agcaataatc
     6841 ttttgtagat tgcacttgct taaaatcccg ccagcggcgg gattttttat tgtccggttt
     6901 aagacaattt aataagaata agctataaaa aaacggcgtc gattgctcaa cgccgtttcg
     6961 tggataacac cgatacggat gttacttctt aatgcccatc tcttcttcaa gccaggcttt
     7021 aaattccgtg ccaagggtgt tatgacgaat accgtattca acgaaggcct gcatgtaacc
     7081 taatttatta ccgcagtcat ggctcttccc tttcatatga taggcttcca ccgtttcttt
     7141 ttcgatcagc atatcaattg cgtcggtgag ctgaatttca tcaccagctc ccggaggggt
     7201 ttttgccagc aacggccaaa tatccgcgct aagtacgtaa cgacccacaa tagcgagatt
     7261 agacggcgca acatccgctt tcggtttttc taccacacca accatcggta cgctttcacc
     7321 cggcgctaat tcaacgcctt tgcaatccac aacgccatat gcggtcacat cagcaaccgg
     7381 ttcaaccatg atctggctat gacccgtttc atcaaagcgg cggatcatct ctgccaggtt
     7441 atcctgtgac aaatcggatt catattcatc cagaataaca tcaggcaaaa taacagctac
     7501 cggttcatca cccactaccg ggtgagcaca caataccgcg tgtcccaggc ctttcgccag
     7561 accctgacga acttgcataa tagtcacgtg cggtggacaa atagactgca cttcatcaag
     7621 cagttgacgt tttacacgtt tttccagcat tgcttccagt tcaaaactgg tatcaaagtg
     7681 gttttcaata gagtttttag atgagtgtgt aaccagcaca atttcagtaa tgccagccgc
     7741 aatacattca ttcacgacgt attgaattaa tggcttatcg acaagtggca gcatctcttt
     7801 cgggatggct ttcgtcgccg gcaacatcct ggttcctaat cccgcaacgg ggataacggc
     7861 ttttttgact ttcgtattaa tggcagccat ttaaattctc ctggactgtt cgtgttttga
     7921 acgtgttcat atttctgtat cgcatcccag tatatcagca ccccgaaggg gctaagttct
     7981 gaaaaggagg cgagttagca taattaagac aattacttat acgaatggcg acgatagtac
     8041 caccagccga cattagcagg taatgcaaat ttagcccgcg ttatcgtttg ctcattctgc
     8101 agacaacatc aagcgcagtc gaccaccggt tccccatatt tggcattgcc aggcatcgca
     8161 tcgctggctc aattgattca aataagcgtt acctaaagtg cctaacggaa caccattact
     8221 gatttgcacc tggtgttcgc cggtatttaa cgtcgcattc agacccgcag aaaccagaat
     8281 gagatttttc agttcgcgat gataatagcc aactaatagc ggaaactgcc ccggcagatt
     8341 ggcctgacga agtaaatggt ttacctgctt caataacgcg cccaactctg gcaaccgttg
     8401 attttggtgt gcaagctgtt cctgtaataa tccgttaaac aatgcgcgta ataacaaggc
     8461 agcaagtacg ccattatgtc cagctcgggt gacatcaagg caataaaatg ccagatcgtt
     8521 ttccgaaagt gcggcaatat caagcaccag gccgggtttg tccgcggcaa ccaattgacg
     8581 ataattaacc cggcaatggg aaatcacctg ctgaaccggc ggttgtagtt cctgtaataa
     8641 tttcgccgct gcggcagggt tatcaaccat tgcatcccag tcgcgaaaaa gcctttcctc
     8701 ttcctcaacg cgcgaattaa acatgctggg atagagacag gcaaaaacca tctcgcgcaa
     8761 gcgattcaga tctttaactg gtttcagcaa aacatcttca acgcccagac gtaacgcttt
     8821 ggcaatatct gccatatttt cagtggcaga tatcaccaga actggggtct ggtcgcctct
     8881 gttacgtata tgctccagca gtttaagccc gttcattcgt ggcatcgcga tatcacatat
     8941 catcaggtct ggagtgaaac ctcccagcaa ctcaagggca tccaccccat cagccgccag
     9001 taccgttgtc gctcccaatg aggaaaacca tgaatccaga agcgagcgaa atacctgctc
     9061 atcttcaaca atgagaatct gttttccgac caatggctgc gtcatgttct ctcccctgac
     9121 tggctttact caatagtggc atgctattgc cacctgcgcc tgtcagaatt aacttaagtg
     9181 tagataaaaa ttctggtcaa atgttggtgc gtaccaacgg caaaagttcg tccattttcc
     9241 tttccactgc cagctgtccg gccgcaatgg cagcgtgcgc gcgatggaaa tcaagcgtag
     9301 atatttgcgg gcaaacaggt tgaatcagaa tatcgggcgg atcacctgcc atgcggttcc
     9361 ttttaaggcg gttctccagc acctggatag aagtggtcat aatctctgtt gccgttggcg
     9421 ctgtcaccgc ccgacgtgtc gttatgctgc ctaacctttc tttcagacgc gcatgccacg
     9481 gcagagaatc accattctcg ctattttctt cactgacatt aaaggagagc aaatcttgtt
     9541 gcatcaaatg agcatcgtgc tgcaggtcaa ccgctatcac aatatcagcc cccaatgcac
     9601 gcgtgaggga aataggaatt gggttaacga ctgctccatc aaccagccag tagccgttat
     9661 gtgcaacagg tgccatgagt cctggaatac tgcatgatgc gcgaatagca agatggagat
     9721 cgccttcagt aaaccataat tcacgtcccg tacttaaatt ggtggcaaca gccgcaaagc
     9781 gacgggaaca attttcgatc tctgtttccg gcattatttc gcggtattga ttgaagacac
     9841 gctcgccgcg cagtaaccca ccgcgctgcc aggagagatc catcaggcgt aaaacatccc
     9901 aataactgaa agaggtcacc caatcttcca gcgcagataa tcgatcgcat gcataggcag
     9961 cgcccaccag cgaaccaatt gaacatcctg caacgatatc aatttcaata cccacttttt
    10021 ttagcgcatt aataacgcca atatgcgacc aacctctcgc cgcgccagat cccagcgcca
    10081 gccctatttt tatctttctc attattcccg caagattccc ctggaatgca atcgtagcca
    10141 catcgctact gctcggttaa catagtgcta ctcaggcggt taatacgccg ttattgtttc
    10201 cagggagatt atttgtgtct cagctttgtc cctgtggtag tgctgtcgag tatagcctat
    10261 gttgccaccc ttatgtgtct ggtgaaaagg ttgcacctga tccagaacat ctcatgcgtt
    10321 cgcgttactg cgcttttgtg atgcaagacg cagattattt aataaagacc tggcatccat
    10381 cttgtggagc agcggcgtta cgtgccgaat tgattgccgg atttgcgcat actgaatggt
    10441 tgggattgac cgtatttgaa cattgttggc aggacgccga caatatcgga tttgtcagtt
    10501 ttgttgctcg atttaccgaa ggtggcaaaa caggtgcaat aattgaacgt tcgcgatttt
    10561 tgaaagaaaa cggtcaatgg tactatattg acggtacacg tccgcagttt ggccgcaacg
    10621 atccctgccc ttgtggttca ggtaaaaaat ttaaaaagtg ctgcggccaa taatggttga
    10681 cggtacggtt tagcaaacac tctcaacaag gtttttccag caatgcattc actccaacgt
    10741 aaagttctgc gtactatttg tccggaccaa aaaggtctga tcgcacgtat taccaatatt
    10801 tgctacaagc acgagttaaa tatcgtacag aacaatgaat ttgttgatca ccgtaccggg
    10861 cgctttttta tgcgcacgga actggaaggg atttttaatg attccaccct gctggcggat
    10921 ctcgatagcg cattgccaga aggctccgtg cgtgagctga atcctgccgg tcgtcgccgg
    10981 atagtgattc tggtcactaa agaagcgcat tgccttggcg atttgttgat gaaagccaat
    11041 tacggcggcc tggatgtcga aatcgcggca gttattggta accacgatac tctacgttct
    11101 ctggttgagc gttttgatat tccgtttgag ctggtaagcc atgaagggtt aagccgcaac
    11161 gagcacgatc aaaagatggc ggatgccatt gatgcttatc aacctgacta cgtggtgctg
    11221 gcgaagtata tgcgggtatt aacgccggaa tttgtggcac gcttcccgaa taagatcatc
    11281 aatattcacc attcattcct gccagcgttt attggcgcac gtccttatca ccaggcctat
    11341 gaacgtggtg tgaagattat tggcgcaact gctcactatg tgaatgacaa tctggacgaa
    11401 ggcccaatca tcatgcagga cgttattcat gtcgatcata cctacacagc tgaagatatg
    11461 atgcgcgcag gtcgtgacgt cgagaaaaac gtcttaagtc gtgcactata caaagtactg
    11521 gcacagcgcg tctttgttta cggtaatcgg acgattattc tttaatcgcc agcaaaaata
    11581 actggttacc tttaatccgt tacggataaa aattacgcaa ccagttcatt tttctcaacg
    11641 taacacttta cagcggcgcg tcatttgata tgatgcgccc cgcttcccga taagggagca
    11701 ggccagtaaa aagcattacc ccgtggtggg gttcccgagc ggccaaaggg agcagactgt
    11761 aaatctgccg tcatcgactt cgaaggttcg aatccttccc ccaccaccat aattcaccac
    11821 agcgatgtgg taacttcggt gagattgtga aagtttgcac tcatcgggga agggtgagaa
    11881 ccttcgacta aggttcgatt cgagcgaaag cgagagaacg ttgccacagg caacggcccg
    11941 aagggtgagg agcgaagcga cgaataatcc ttcccccacc accatctcaa gcattacctc
    12001 gaaatttgaa ttacccctgg tggggttccc gagcggccaa agggagcaga ctgtaaatct
    12061 gccgtcatcg acttcgaagg ttcgaatcct tcccccacca ccatcacttt caaaagtccc
    12121 tgaactctca aacgaatccg taattaaata ttctgcccaa gcggggaagg atgagaagct
    12181 tcgaccaagg ttcgacttga gcgccagcga gagagcgttg ccgcaggcaa cgacccgaag
    12241 ggcgaagcgc gcagcgctga gtaatccttc ccccaccacc atcacttcct gaaatactca
    12301 aaacctgtta ctccgagcta attttgcccg atggcaccgc gtttaccgga cctacaatat
    12361 cctcccctgt aggtcggata agcgaagcgc atccgacaac tcaccccgca ccgtgcccat
    12421 aaaaaaaccc cgccgaagca gggtctatgt gaactaaaat tcgcttagtg acgagcgcgc
    12481 accaactgat acttacgtgt cagatactcc accggtacgc tccagatgtg caccagacgc
    12541 gagaacggga acagcaggaa taacgtcatc cccaacacca ggtgcagacg gaagataaac
    12601 gccacaccat cgaggtgttg agaagcgcca ccgtggaagg tcaccaccga ctgcgcccag
    12661 ccaaccagtt tcatcatctc gctaccgtcc atatgctgag cggagaacgg aatggtcaac
    12721 aggcccagcg cgcactggat aacgagcagc gacaggatca ggatatccgc tccggtagtg
    12781 gttgcacgca cgcgtgggct gaacagacga cgcttcagca acagcacgcc gccaatcaga
    12841 cacagcacgc cgctggcact accagcaaac atcgccattt tctgtttcac ttcaatcggc
    12901 agccaggctt catacatcca gtgcggcgtc agcataccga agaagtgacc aacaaaaatc
    12961 cccagaatac cgatatggaa caggttcgac gccaggttca tccctttgcg atccagcatc
    13021 tggctggacg ccgcgcgcca ggtgtactgc ccgtagtcat aacgcagcca gctaccaatc
    13081 aggaagaccg ccccggctat gtacgggtag atatcaaaga agaacatatt caggaattgc
    13141 attagtgctg tcctccggtg gtgatattca gatattgcgg cgcgacggca ccggcaaagc
    13201 gacgctgatg agcagtgatt gctgaatcac cgcagccttt gtcagcaaag aatttaacct
    13261 gctcttcttc ccagacagca tccagcgcct gcggcgtatc atcgcgcgct tcgtcggcaa
    13321 ttttttccgc cactttgtcg ctgtcgatag cggtattggc caatttcagc agcagatcaa
    13381 acatcacggc ataacggctt tcacgctgtt gcagacgcgc gctcagcaat gccagaatcg
    13441 gcgcgatatc tttcaaacct tccacggctt cgctttgcgg cagctgcgac aggtactcca
    13501 gatacagcgg cagatggtcc ggtaattcgc ggctgtttaa ctgcaaaccg tgctgctcgt
    13561 actgcgccag caggtccacc atcgcctggc cgcggtcacg ggattcaccg tgaacatgtt
    13621 caaatagcaa cagtgacgtg gcgcggccac ggtcgaacag ttcgctgtac tgggcctggg
    13681 catcgagcgg atccatcgcc gttaaatcgc gcaggaaaat gcccagcgca tgggcatctt
    13741 ctttcgacag atttttcgac gcagcaatcg cctcaaacat ctcttgttga tgctgccata
    13801 aggcagcatc cggatattca aggagacgcg atacaatcac gagttcgatc atggatgcgg
    13861 ctccgttttg ctggtcacat cgatggcatc gatacgacgg ctgttgaaca gattgaattt
    13921 ggtatctgaa ccgtggcagc catcaccaaa ggtaaagccg cagccatttt tctccgggaa
    13981 ggcttcccgt gccagttcac gatgactact cggcaccaca aagcgatctt cgtagttagc
    14041 aatcgccaga taacggtaca tctcctgtgc ctgggcttcg gtcagaccga cctcttccag
    14101 cgcacgggta tcaactttac cgtcaacggt ttcagcacgt ttgtaatgac gcatcgccag
    14161 catacgtttc agtgcgcgca gtaccggttt ggtatcaccg gcggtcagca gattcgccag
    14221 atactgtacc ggaatacgca agctttcgac gtctggcaga atgccgttgc tacccagctc
    14281 acccgcgtct gctgcagact gaatcggaga cagaggcggc acgtaccaga ccatcggcag
    14341 tgtgcgatat tctggatgca gcggcagcgc cagtttccat tccattgcca ttttataaac
    14401 cggcgactgc tgtgcggctt caataacgct cagcggaata ccgtctttaa tcgcctgctc
    14461 gatgactttc ggatcgttcg gatcgaggaa cacctccagc tgacgctggt aaagatcttt
    14521 ctcgttctcg gtgctggctg cacgttcaat cgcgtcggca tcgtacagca gcacgccaag
    14581 ataacggata cgaccgacac aggtttctga gcacacggtc ggctgacccg cttcaatacg
    14641 cggatagcag aagatgcact tctcagactt accgctcttc cagttgaagt agattttttt
    14701 gtacgggcat ccggtgatgc acatacgcca gccacggcat ttatcctggt cgatcaggac
    14761 gatgccatct tcttcacgct tgtaaatcgc accgctcggg caggtcgcca cacatgccgg
    14821 gttcaggcag tgttcgcaca ggcgcggcaa atacatcata aaggtgtttt cgaactggct
    14881 atacatcgcc ttctggatgt tgtcgaagtt cttgtctttc gccagtttgt caaactcacc
    14941 acccaggtca tcttcccagt tcggcccttt ttcgattttc gccatccgtt ccccggtaat
    15001 cagcgaacgc ggacgggcaa tcggctgcga tttgctgcct tccggcgcgg tatgcaggtt
    15061 ctgataatca aaatcgaacg gctcgtaata atcgtcgatc cccggcagat gcgggttagc
    15121 gaagatttta cccagcagca tggcgcggtt acccatgcgc ggctgcagtt tgccattgat
    15181 tttacggatc cagccgcctt tgtatttttc ctggttttcc cagtcagtcg ggaagccctg
    15241 acccggcttg gtttccacgt tgttgaacca cgcgtattcc acgccttcac ggctggtcca
    15301 gacgttttta caggtaactg aacaggtgtg gcagccgatg cacttatcga gattcagcac
    15361 catgccgact tgtgaacgaa ttttcatttt acgctctcct gtacctggtc attgccttcg
    15421 ccatctaacc agtcaatgtt cttcatctta cgcactacaa caaactcatc gcggttagaa
    15481 cctacggtgc cgtagtagtt aaagccgtat gccagatggg catagccgcc gatcatatgc
    15541 gtcggtttcg gcgtgatacg ggtgaccgag ttatggatac caccacgctg ttgggtaatt
    15601 tccgaaccag gcaggttaac gatacgttcc tgcgcgtggt acatcatggt catccctgcc
    15661 ggaacacgct ggctgacaac cgcacgggca gtcagagcac cgttgctgtt gaatacttca
    15721 atccagtcgt tatcggcgat acccagatct ttggcatcgg cttcactcaa ccagaccacc
    15781 ggaccaccgc gacctaaagt cagcatcagc aggttgtcgc tgtaggtgga gtggataccc
    15841 cacttctggt gcggcgtcag gaagttgagc gctttttccg ggttgccgtt ggatttctgg
    15901 cctatcactt ctttcaccga acgggtgtcg atcggcggac gataaaccag caggctttca
    15961 ccgaaatcac gcatccactg gtgatcctga tacagttgct gacgaccaga gagcgtacgc
    16021 catgggatca gctcgtgaac gttggtgtaa ccggcgttgt aagaaacgtg ttcatcttcc
    16081 agaccagacc aggtcgggct ggagataatt ttgcgcggct gtgcctgaat atcgcggaag
    16141 cggatcttct cgtcttcttt attcagcgcc agatgcgtat ggtcacgacc ggtaaattcg
    16201 ctcagggcag cccaggcttt cacggctacc tgaccgttgg tttccggtgc cagtgtcagg
    16261 atcatctctg ccgcatcaat tgcggtgttc agcatcggct ggcctttcgc cggaccttcc
    16321 gctttggtgt agttgagctt acgcagcaga tccatctcgc tctgggtgtt ccaggcaatc
    16381 cctttaccgc cgttaccgat tttctccatc agcgggccga tagaagtaaa gcgttcgtaa
    16441 gttgccggat aatcgcgctc tacgaccata atgtgcggtg cagttttgcc tgggatcagg
    16501 tcgcattcgc cttttttcca gtctttcaca tccagcggct gcgccagttc agcggcagag
    16561 tcatgctgga taggcagcgt gacgatgtcg gtttctttac ccagatggcc gacgcacact
    16621 tcggagaatt tcttcgcgat ggctttgtag atttcccagt cgcttttcgc ttcccaggcc
    16681 ggatcgaccg ccgcagacag cgggtgaata aacggatgca tatccgaagt attcatgtcg
    16741 tctttttcgt accaggtcgc cgtcggcaaa atgatgtcgg aatagagaca ggtgctcgac
    16801 agacggaagt ccagcgtaac caccagatcc agcttgcctt ccagaccatt gtcctgccag
    16861 tccacttctt ccggcttcac gccgccctgt tgccccagat ctttaccctg gataccgtgc
    16921 tccgtcccca gcaggtactt gagcataaac tcatgacctt taccggaaga accgagcagg
    16981 ttagaacgcc agatgaacag gttgcgcggg tggtttttac cgttttctgg ttgttctgcc
    17041 gcaaaacgga tggaaccctc tttcagggat ttcaccgtat agtccaccgg attcatcccg
    17101 gctttttccg cttcgccagc gatagtcagc gggttagtgc ctaactgcgg tgcagacggc
    17161 agccagccca tgcgttccgc acggacgtta aagtcgatca agtgtccggt atagcgggat
    17221 ttgtccgcca tcggtgacag caactcttcc gccgtgacgg tttcataacg ccactggctg
    17281 gagtggttat agaaataaga agtgctgttc atgtgacgcg ccggacgctg ccagtcaagg
    17341 gcaaacgcca gcggctgcca gccggtttgc ggacgcagtt tttcctgacc tacatagtgc
    17401 gcccagccgc ccccgctctg accgacacag ccgcagaaaa tcagcatgtt gatcagacca
    17461 cgatagttca tatcgaggtg ataccagtgg ttcagccccg caccgacgat aatcatcgaa
    17521 cgaccgtgcg ttttatcagc gttatcggca aattcacggg cgatgcgaat aatctggctg
    17581 cgagaaacgc cggtaatctg ctcggcccag gccggggtat aagctttcac atcgtcatag
    17641 ctggttgcac agttaacgtc gttcaggcca cgttccagac cgtagtttgc cagcgtcaga
    17701 tcataaacgg tggtcaccag ggcggtgctg ccatcagcca gttgcaggcg tttcaccggc
    17761 agtttgtgca gcagcacgtt ttccagttcc actttgttga agtgttccgt gccgtcgcca
    17821 ccaaagtacg ggaagcctac ctcagcgatc tcatcctggc tacccagcag gctcagttgc
    17881 agctcggttt cttcgccagt tttgccgtcg cgctgctcaa gattccattt gcccttctcg
    17941 ccccagcgga agccaataga accgttcggc gcaaccattt cgccattggt attaaaggcg
    18001 acagttttcc attccggatt gttttcctgg cccagcgcat caaccagatc agcagcgcgc
    18061 aacatacgac ctgcagcgta gtaaccgtcg cgttcttcca gcatcaccag catcggcatg
    18121 tcggtgtagc gacgcacata gtcggtgaaa tactggcttg ggttgtcgag gtggaattca
    18181 cgcagcatta cgtggcccat cgccagcgcc attgccgcat cggtgccctg tttcggtgcc
    18241 agccacagat cgcacagttt ggcgatttca gcgtagtctg gtgtgacggc aacagttttg
    18301 gtgcctttgt aacgcacttc agtaaagaag tgagcatccg gggtacgcgt ctgcggcacg
    18361 tttgaacccc aggcgatgat gtagctggag ttataccagt cagcagattc cggtacgtca
    18421 gtttgctcgc cccaggtttg cggagacgca ggaggcaggt cgcagtacca gtcgtagaag
    18481 cttaagcaag taccgccaat cagcgagaga tagcgtgcac ccgatgcgta agaaaccatc
    18541 gacattgccg gaattggcga gaaaccagca acacggtccg ggccgtagtt tttgatggtg
    18601 taaacgttag atgcggcgat cagttcgttc acctcctgcc aggaagaacg aacaaatcca
    18661 ccgcgtccac gcgcctgctt aaagcttttc gctttatcgg cgtcttcaat gatagaagcc
    18721 catgcctcaa ccggatcgct atgcagcgcc ttcgcttcac gccacatttt catcaggcgt
    18781 ttgcgcatca tcgggtattt caggcggttg gcactgtaaa gataccagga gtagctggca
    18841 ccgcgcgggc agccgcgagg ttcatggttt ggcagatccg gacgggtacg cggatagtca
    18901 gtctgctggg tttcccaggt gaccagaccg tttttgacgt agattttcca gctgcaggag
    18961 ccggtgcagt ttaccccgtg ggtagagcgg acgattttgt catgctgcca acgctggcga
    19021 tatccatcct cccagtcacg gttggtattg agaagctggc catgcccatc ggcaaaggtt
    19081 tcacccttct gtttgaagta gcgaaaccgg tccaggaatt tactcatcgg ttttctcctg
    19141 tgggagcctg tcggccctct gataaatcga cattgctaat gtttaagggc aaggtaacgc
    19201 tctgaaacag cgtgggaatt gataacgatc aagagtgatg gggatgaaaa ataaagtaaa
    19261 ttcctaagat acccctttag gataa
//
LOCUS       DAPHBJ010000079        19264 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.079, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000079 DAPHBJ010000000
VERSION     DAPHBJ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.079"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            441..1580
                     /gene="wza"
                     /locus_tag="RHZ76_002442"
     CDS             441..1580
                     /gene="wza"
                     /locus_tag="RHZ76_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HDV2813065.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1586..2029
                     /gene="wzb"
                     /locus_tag="RHZ76_002443"
     CDS             1586..2029
                     /gene="wzb"
                     /locus_tag="RHZ76_002443"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HDV2813066.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            2032..4194
                     /gene="wzc"
                     /locus_tag="RHZ76_002444"
     CDS             2032..4194
                     /gene="wzc"
                     /locus_tag="RHZ76_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HDV2813067.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITTVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4287..5126
                     /gene="wcaA"
                     /locus_tag="RHZ76_002445"
     CDS             4287..5126
                     /gene="wcaA"
                     /locus_tag="RHZ76_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HDV2813068.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5129..5617
                     /gene="wcaB"
                     /locus_tag="RHZ76_002446"
     CDS             5129..5617
                     /gene="wcaB"
                     /locus_tag="RHZ76_002446"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HDV2813069.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5614..6831
                     /gene="wcaC"
                     /locus_tag="RHZ76_002447"
     CDS             5614..6831
                     /gene="wcaC"
                     /locus_tag="RHZ76_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HDV2813070.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTAGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAGFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6806..8023
                     /gene="wcaD"
                     /locus_tag="RHZ76_002448"
     CDS             6806..8023
                     /gene="wcaD"
                     /locus_tag="RHZ76_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HDV2813071.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8034..8780
                     /gene="wcaE"
                     /locus_tag="RHZ76_002449"
     CDS             8034..8780
                     /gene="wcaE"
                     /locus_tag="RHZ76_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HDV2813072.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAAYF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8796..9344
                     /gene="wcaF"
                     /locus_tag="RHZ76_002450"
     CDS             8796..9344
                     /gene="wcaF"
                     /locus_tag="RHZ76_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HDV2813073.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQSTIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETK"
     gene            9371..10492
                     /gene="gmd"
                     /locus_tag="RHZ76_002451"
     CDS             9371..10492
                     /gene="gmd"
                     /locus_tag="RHZ76_002451"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HDV2813074.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10495..11463
                     /gene="fcl"
                     /locus_tag="RHZ76_002452"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10495..11463
                     /gene="fcl"
                     /locus_tag="RHZ76_002452"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDV2813075.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            11463..11942
                     /gene="gmm"
                     /locus_tag="RHZ76_002453"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11463..11942
                     /gene="gmm"
                     /locus_tag="RHZ76_002453"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HDV2813076.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDTLLASNDVHANSRAYFLAEKRAGVPGL"
     gene            11939..13162
                     /gene="wcaI"
                     /locus_tag="RHZ76_002454"
     CDS             11939..13162
                     /gene="wcaI"
                     /locus_tag="RHZ76_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HDV2813077.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWCYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     NKENVLRQFINDIRG"
     gene            13165..14601
                     /gene="cpsB"
                     /locus_tag="RHZ76_002455"
     CDS             13165..14601
                     /gene="cpsB"
                     /locus_tag="RHZ76_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HDV2813078.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14703..16073
                     /gene="cpsG"
                     /locus_tag="RHZ76_002456"
                     /gene_synonym="manB"
     CDS             14703..16073
                     /gene="cpsG"
                     /locus_tag="RHZ76_002456"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HDV2813079.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDIRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKMLTPLKLVINSGNGAAGPVVDAIEARFKALSAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGMPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKEKTLGELVRDRMAAFPASGEINSKLAHPIEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16128..17522
                     /gene="wcaJ"
                     /locus_tag="RHZ76_002457"
     CDS             16128..17522
                     /gene="wcaJ"
                     /locus_tag="RHZ76_002457"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HDV2813080.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17524..19002
                     /gene="wzxC"
                     /locus_tag="RHZ76_002458"
     CDS             17524..19002
                     /gene="wzxC"
                     /locus_tag="RHZ76_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HDV2813081.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDLI
                     GDMLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRSGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
BASE COUNT         4619 a         4804 c         5326 g         4515 t
ORIGIN      
        1 taaaaacaat attgaatgac acttaatata attcttaaaa atagccaatt gccgaattgt
       61 taccttgcct gctattccgt tagctgtaac acttcctcct gcattattgg agagccaata
      121 ttcaattacc ataatgtcac cttatattcg gaagttaatt tccgcaataa ccctttctca
      181 tcaacgattg tatgcagaga gggtttgaaa aattaagcta aatcagtgta ttggtagcta
      241 gaaagccagg ggcggtagcg tgtctggatg cctgaaagac cagtctgaat tattctgtca
      301 atagcctacg gatgaaatca atttttttag gactgatgcc agttaaattt ttattcaatt
      361 aactgcaagg taaatgtgca tggcgacatt gctattaata gtgcacagga taattactct
      421 gccaaagtga taaataaaca atgatgaaat ccaaaatgaa attgatgcca ttattggtgt
      481 cagtaacctt gataagcggt tgcacagtac ttccgggcag caatatgtcg acgatgggca
      541 aagacgtcat caaacagcag gacgctgatt tcgatctcga caaaatggtg aatgtttatc
      601 cgctgacccc gcgcctgatt gaccaattac gcccacgccc gaatgtagcg cgccccaata
      661 tgacgctgga aagtgagatc gcgaattacc agtatcgcgt cgggccgggg gacgttctta
      721 atgtcaccgt ctgggatcac ccggaactca ccacgccagc cggtcagtac cgcagctcca
      781 gcgacaccgg caactgggta cagcctgacg gcactatgtt ttacccgtat atcggcaagg
      841 tccacgtagt cgggaaaacg ctcgctgaaa tccgcagtga tattaccggg cgcttagcga
      901 cgtacatcgc tgacccgcag gtggacgtta atatcgccgc cttccgctca caaaaggcct
      961 atatctccgg tcaggtgaat aaatccggtc aacaggcgat caccaacgtg ccactgacta
     1021 ttctcgacgc catcaacgcc gcaggtggcc tgaccgacac cgctgactgg cgcaacgtgg
     1081 tgttaacaca caatggtcgt gaagaacgca tttctttgca ggcgctgatg caaaacggcg
     1141 acctcaacca gaatcgactg ctttaccccg gcgatattct ctacgtgccg cgtaatgatg
     1201 atctgaaagt gtttgtgatg ggtgaagtga agaaacagag caccctgaaa atggacttta
     1261 gcggtatgac cctgactgaa gccctgggca atgctgaagg tatcgacatg accacctcca
     1321 acgccagcgg catctttgtc attcgtccgc tgaaaggcga gggcgggcgt aacggcaaga
     1381 ttgccaatat ctaccagctg gatatgtccg atgccacgtc gctggtgatg gcgacagaat
     1441 tccgcctgca accttatgac gtggtgtatg tcaccaccgc cccggtttcc cgctggaacc
     1501 gtctgatcaa tcagttgctg ccaactatta gcggtgtccg ttacatgacg gatacagcca
     1561 gcgacattca taactggtaa tcgtcatgtt taacaacata ttagttgtct gtgtcggcaa
     1621 tatttgccgt tccccgacgg cggaacgctt actgcaacgt tatcacccgg aactgaaagt
     1681 ggagtccgct ggactcggcg cgctggtcgg taagggcgct gatcctaccg ctatcagcgt
     1741 cgccgcagaa catcaactgt ctctggaagg ccactgtgca cgacaaatca gccgccgcct
     1801 gtgtcgcaac tatgacctga ttttgaccat ggaaaagcgc catatcgaac gcttatgcga
     1861 gatggcacct gagatgcgcg gcaaagtgat gctgtttggt cactgggata acgaatgtga
     1921 aatccccgat ccgtatcgca aaagccggga aacgtttgca gcggtgtaca cattacttga
     1981 acggtctgcc cgccagtggg cgcaggcatt gaacgcagag caggtataag aatgacagaa
     2041 aaagtaaaac aacatgccgc tccggtaacg ggcagtgatg aaatcgatat tggtcgcctg
     2101 gtcggcaccg tcattgaagc gcgctggtgg gtgattggca tcaccaccgt attcgccctc
     2161 tgtgccgtgg tttacacctt cttcgccacg ccgatttata gtgccgacgc actggtacaa
     2221 atcgagcaaa acagcggcaa ttcgttagtg caggacattg gttcggcatt agccaacaaa
     2281 ccgcctgcat cggacgccga gatccagttg attcgttcgc gtctggtgct tggtaaaacg
     2341 gtggacgatc tcgacctcga tattgcagtg agcaaaaaca cgttcccgat tttcggtgcg
     2401 ggctgggatc gcctgatggg acgtcagaac gagacggtga aagtgaccac ctttaaccgc
     2461 ccgaaagaga tggcggatca ggtgtttacg cttaatgtgc tggacaacaa aaactacacc
     2521 ctgagcagcg acggcggctt tagcgcccgg gggcaagcgg gccagatgct gaaaaaagaa
     2581 ggcgtcacgc tgatggttga agccattcac gccagcccgg gcagtgagtt taccgtcacc
     2641 aaatactcca cgctggggat gatcaaccaa ctgcaaaaca gcctgacggt aacggagaac
     2701 ggcaaagacg ctggcgtttt gagcctgact tataccggtg aagatcgtga acagatccgc
     2761 gacattctta acagcatcgc ccgtaactat caggaacaaa atattgagcg caaatcggcg
     2821 gaagcgtcga aaagcctcgc cttcctcgcg caacagttac cggaagtacg tagccgcttg
     2881 gatgttgccg aaaacaaact gaatgccttc cgtcaggata aagattctgt tgatctgccg
     2941 ctggaagcaa aagcggtgct cgattcgatg gtgaacatcg atgcgcagtt gaacgaactg
     3001 acctttaaag aggcggaaat ctccaagctg tacaccaaag ttcacccggc gtaccgcacg
     3061 ctgctggaga aacgtcaggc gctggaagac gaaaaagcca aacttaacgg tcgcgtaacg
     3121 gcgatgccga aaacccagca ggagattgtc cgtctgaccc gcgatgtcga gtctggtcag
     3181 caggtctata tgcaactgct gaataaagag caggagctga aaatcaccga ggccagcacc
     3241 gtcggcgatg tgcgcattgt tgacccggca atcactcagc ctggcgtgct gaaaccgaag
     3301 aaagggctga ttatcctcgg cgcgattatc cttggcctga tgctttctat cgtgggtgtg
     3361 ctgctgcgct cgttgtttaa tcgcggcatt gaaagcccgc aagtactgga agaacacggt
     3421 attagcgtct atgccagcat cccgctgtcg gaatggcaga aagcgcgtga tagcgtcaaa
     3481 accatcaaag ggattaaacg ctataaacag agccagctac tggcggtggg gaatccaacc
     3541 gatctggcga ttgaagccat ccgtagtctg cgtaccagtt tgcacttcgc gatgatgcag
     3601 gcacagaaca atgtgttgat gatgaccggg gttagcccgt caattggtaa aacctttgtc
     3661 tgcgccaacc tggcggcggt gatcagccag accaataaac gcgtgttgtt gatcgactgc
     3721 gatatgcgca aaggctacac ccacgagctg ttgggcacta ataacgttaa tggcctgtcg
     3781 gaaattctga ttggtcaggg cgatattact accgctgcta aaccgacctc tattgccaaa
     3841 tttgacctga tcccgcgcgg tcaggtgccg ccaaatcctt ctgaactgtt gatgagcgaa
     3901 cgctttgccg aactggtgaa ctgggcgagt aaaaactatg acctggtgtt gattgatacg
     3961 ccgccgattc tggcagtgac cgatgcggca attgttggtc gtcatgttgg aaccacgtta
     4021 atggtggcgc gttatgcggt caacacattg aaagaagtgg aaaccagtct gagccgcttt
     4081 gagcaaaacg gtattccggt gaaaggggtg attctgaact ccatcttccg ccgcgccagc
     4141 gcgtatcagg attatggcta ttacgaatac gaatataagt cggatgcgaa ataacgacgc
     4201 ctgcattcgc accgccccgt aggccggata aggcgttcac gccgcatccg gcaagcaaac
     4261 cagctcataa gccgggagaa caacctatga aagacaatcc gctgatctca atctatatgc
     4321 cgacctggaa ccgccaacaa ctggcgattc gggcaataaa atcggttttg cgccaggact
     4381 acagcaactg ggagatgatc atcgtggatg attgctccac ctcctgggaa caactgcaac
     4441 agtacgtcac cgctctcaac gatccgcgta tcacttacat tcacaacgac attaacagcg
     4501 gggcgtgcgc ggtacgtaac caggcgatta tgctggcgca aggggaatat atcaccggga
     4561 ttgatgacga tgacgaatgg acacccaacc gtctgagcgt cttcctcgcc cataaacagc
     4621 aactggtcac acacgccttt ttgtacgcta acgactacgt ttgccagggc gaagtatatt
     4681 cccaaccggc aagcttgccg ctgtatccca aatcgccgta ttcacgccgc ctgttctaca
     4741 aacgcaatat cattggcaac caggtcttta cctgggcatg gcgtttcaaa gagtgtttgt
     4801 tcgataccga actgaaagcg gcacaggatt acgacatctt cctgcggatg gtggtggagt
     4861 acggcgaacc gtggaaagta gaagaggcga cgcagatcct gcatatcaat cacggtgaaa
     4921 tgcagatcac ctcgtcaccg aaaaaattct ccgggtactt ccacttttac cgcaagcaca
     4981 aagacaaatt cgaccgcgcc agcaaaaaat atcagctgtt taccctctac cagatccgca
     5041 ataaacgtat gacctggcgc actttgctga cgctgctgtc ggtgcgtaac ggcaaacgtc
     5101 tggctgatgg tattcggggg cgctaaagat gctggaagat ctgcgcgcca acagctggag
     5161 tttacgcccg tgctgcatgg ttcttgccta tcgtgttgct catttttgtt cggtgtggcg
     5221 caaaaagaac gtcctcaaca atctgtgggc ggccccgctg ctggtgctgt atcgcattat
     5281 caccgaatgc tttttcggtt atgaaatcca ggctgccgcg accattggcc gccgctttac
     5341 tatccatcac ggttacgccg tcgtgatcaa taaaaacgtg gtagcggggg atgattttac
     5401 cattcgccac ggcgtcacta tcggcaatcg tggtgccgat aacatggcat gtccacacat
     5461 tggcaacggc gtcgaactcg gtgccaacgt cattattctt ggtgatatca cgcttggtaa
     5521 caacgtcacc gtgggcgcgg gcagcgtggt gctcgactct gtcccggaca acgcgctggt
     5581 ggtgggagaa aaagcgcgag tgaaggtaat taaatgaata ttttgcaatt taatgtgcga
     5641 ctggcggaag gcggggcagc aggtgtggcg ttagatctcc accagcgtgc gctgcaacag
     5701 gggctggcgt cacattttgt ctacggttac ggcaaaggcg gcaaagagag cgtcagccat
     5761 cagaactatc cgcaggtcat caaacatacg ccgcggatga ccgcgatggc gaatattgct
     5821 ctgtttcgtc tgtttaatcg cgatctgttt ggcaatttca atgagttata tcgcaccatt
     5881 actcgcacag cgggtccggt ggtcctgcat tttcatgtgc tgcacagcta ctggctaaat
     5941 cttaagagcg tggtgcgctt ttgcgaaaaa gtgaaaaacc ataaaccgga cgtcactctg
     6001 gtctggacgc tgcacgacca ctggagtgtt accggacgct gcgcctttac cgacggttgc
     6061 gaaggctgga aaacaggctg ccagaaatgc ccgaccttaa ataactatcc gccggtgaag
     6121 attgatcgcg cgcaccagct ggtggcgggc aaacgccagt tattccgcga gatgctggca
     6181 ctgggctgtc agtttatttc ccccagccag catgtggctg atgctttcaa tagtctgtac
     6241 gggccagggc gttgccggat tatcaataat ggcattgata tggcaaccga agcgattctg
     6301 gcggacttgc ctccggtgcg cgaaacccag ggtaagccga aaatcgcggt ggtggcgcat
     6361 gacctgcgtt acgacggcaa aactaaccag caactggtgc gcgagatgat ggcgctgggc
     6421 gacaaaatcg aactgcatac ctttggtaag ttctcgccgt tcaccgctgg caacgtggtt
     6481 aatcacggct ttgaaactga caagcgcaag ttgatgagcg cgctcaatca gatggatgcg
     6541 ctggtgttca gttctcgcgt cgataactac ccgctgattt tgtgtgaggc gctatcgatt
     6601 ggcgtgccgg tgattgccac ccatagcgac gcggcgcggg aagtgttgca aaaatccggc
     6661 ggtaaaaccg tcagcgaaga agaggtgctg caactggtgc aattaagcaa accggaaatc
     6721 gcgcaggcga tatttggtac cacgttggcc gggttcagcc agcgcagccg cgccgcctac
     6781 agtggacaac agatgctgga ggagtatgtc aacttctatc agaatctgta gctacctgct
     6841 gctgccgctg atctacctgc tggttaacgt caaaatcgcc cagcttggtg aaagtttccc
     6901 catcactatc gtcacttttt tacctgtctt gttactgctg tttttagaac gcatcagcgt
     6961 taaaaaattg atgattgcct tagggattgg cgcgggactc acggcgttta actacctgtt
     7021 tggtcagtcg ttggatgcca gtaaatacgt cacttcaacc atgctgtttg tctatattgt
     7081 gatcattatt ggcatggtgt ggagtattcg ctttaaaacg atttcgccac acaaccatcg
     7141 caagatatta cgtttctttt atctggtggt cgggctggtg gtggcgctgg cggcggtgga
     7201 gatggcgcaa attatcctca ccggtggcag tagtattatg gagtcgattt cgaaatatct
     7261 gatttacagc aacagctatg tgctgaattt catcaaattc ggcggcaagc gcacgacagc
     7321 actttatttc gaaccggcat ttttcgctct ggcattaatc tcaatttggc tcagcatcaa
     7381 acagtttggt atcaaaacgc ctaaaaccga tgctatgatt ctcgcaggga taatattatc
     7441 cggatcgttt tcaggggtta tgacctttat cctgttttat ttgctggagt gggcattcca
     7501 atatctgaat aaagaggcga ttaagaaaaa gttaccgtta gcattgattt ctctggctgt
     7561 attcctggtt ggcgtggtaa tcgcgtttcc ttatatttcc acccgtctgg gcgatttagg
     7621 tacagaagga tcgtcatcgt attatcgcat tgtcggtccg ctggtgatgg tcggttattc
     7681 tttgacccat attgacggtg tagtcagatt tggctcactt tatgaatatg tcgcatcatt
     7741 cggaatattt aacggtgcgg atgtcggaaa aaccatagac aatggtttgt atctgctgat
     7801 tatttatttt tcctggttcg cagtgttttt atcgctgtgg tacatgggga aagtgataaa
     7861 aatgatgatc aacgcttttg gtgataaccg caattttcgc gtgcaattat atctttttac
     7921 tccggtgtcg ctgtttttta ccggttcgat atttagcccg gaatatgcat ttttaatcgt
     7981 ctgtccgttt attttgcgaa aagcgttaaa tattacgagg taagaataag aacatgttgc
     8041 ttagcataat cactgtcgcg tttcgtaacc tcgaagggat agtcaaaaca catgcctcgc
     8101 tggcgcatct ggcgcaggcg gacgatatca gcttcgaatg gattgttgtc gatggcggct
     8161 ccaatgacgg cacccgtgag tacctggaaa atctcaatgg tatctataac ctacgctttg
     8221 tcagcgagcc agataacggt atctacgacg ccatgaacaa gggcattgcg atggcacaag
     8281 gcaagttcgc gttgtttctc aactcgggcg atatttttca tcaggatgcc gcatattttg
     8341 tccgtaagtt aaaaatgcaa aaagataacg tgatgatcac cggcgatgcg ctgctggatt
     8401 ttggcgacgg gcataaaatt aaacgtagcg ccaaaccggg ctggtatatt tatcacagcc
     8461 tgcccgccag ccatcaggcg atatttttcc cggtatccgg cttgaaaaaa tggcgttatg
     8521 acctggaata taaagtttct tccgactacg cgctggcagc caaaatgtat aaagccggtt
     8581 atgcatttaa aaaactcaat ggcctggtgt ctgaattttc tatgggtggg gtatcaacca
     8641 ccaataatat ggaattgtgt gctgacgcca aaaaagtcca acggcaaata ttacatgtgc
     8701 ctggcttctg ggctgaatta tcctggcatt tacgccaacg tactacctca aagacgaaag
     8761 ccttatataa caaaagctga atataaggga aaacgatgca agatttaagc ggtttctcgg
     8821 tgccgaaagg gtttcggggc ggcaacgcta ttaaagtgca attatggtgg gcagtacagt
     8881 caacaatatt tgcctggtcg ccacaagtat tgtatcgctg gcgggctttt ttattacgtt
     8941 tattcggcgc aaaaatagga aaaaacgtag ttattcgtcc gtcagtaaaa attacctatc
     9001 cgtggaaatt aaccttaggt gattacgcgt gggtcggcga tgacgtcaat ttatataccc
     9061 tcggtgaaat aaccattggc gcacattcgg tgatatcgca aaaaagttat ttatgcaccg
     9121 gtagccacga ccatgcaagt caacatttca ccattaatgc cacgcctatt gtgattggcg
     9181 agaaatgctg gctggcaacc gatgtttttg ttgcccctgg cgtcacgatt ggcgacggca
     9241 ccgtcgtggg tgcacgaagc agtgttttta aatcgcttcc ggcaaatgtg gtttgccggg
     9301 ggaatcccgc agtggtgata cgcgaacgcg ttgaaactaa ataaattcaa aaaatacaga
     9361 ggaataagac atgtcaaaag tcgctctcat caccggtgta accggacaag acggttctta
     9421 cctggcagag tttctgctgg aaaaaggtta cgaggtgcat ggtattaagc gtcgtgcatc
     9481 gtcattcaac accgagcgcg tggatcacat ttatcaggat ccgcacacct gcaacccgaa
     9541 attccatctg cattatggcg acctgagtga tacctccaac ctgacgcgca ttttgcgtga
     9601 agtgcagccg gatgaagtgt acaacctggg cgcaatgagc cacgttgcgg tttcttttga
     9661 gtcaccggaa tataccgctg acgtcgacgc gatgggtacg ctgcgcctgc tggaggcgat
     9721 ccgcttcctc ggtctggaaa agaaaacccg tttctatcag gcttccacct ctgaactgta
     9781 cggtctggtg caggaaattc cgcagaaaga aaccacgccg ttctacccgc gatctccgta
     9841 tgcggtcgcc aaactgtatg cctactggat caccgttaac taccgcgaat cctacggcat
     9901 gtacgcctgt aacggtattc tcttcaacca tgaatccccg cgccgcggcg aaaccttcgt
     9961 tacccgcaaa atcacccgcg caatcgccaa catcgcccag gggctggagt cgtgcctgta
    10021 cctcggcaat atggattccc tgcgtgactg gggccacgcc aaagactacg taaaaatgca
    10081 gtggatgatg ctgcaacagg aacagccaga agatttcgtt atcgcgaccg gcgttcagta
    10141 ctccgtacgt cagttcgtgg aaatggcggc agcacagctg ggcatcaaac tgcgctttga
    10201 aggcacgggc gttgaagaga agggcattgt ggtttccgtc accgggcatg acgcgccggg
    10261 cgttaaaccg ggcgatgtga ttatcgccgt tgacccgcgt tacttccgtc cggcggaagt
    10321 tgaaacgctg ctcggcgacc cgaccaaagc gcacgaaaaa ctgggctgga aaccggaaat
    10381 caccctcaga gagatggtgt ctgaaatggt ggctaatgac cttgaagcag cgaaaaaaca
    10441 ctctctgctg aaatctcacg gctacgacgt ggcgatcgcg ctggagtcat aagcatgagt
    10501 aaacaacgca tttttatcgc tggtcatcgc gggatggtcg gttctgccat caggcgacag
    10561 ctcgaacagc gcggtgatgt ggaactggta ttacgcaccc gcgacgagct gaacctgctg
    10621 gacagccgcg ccgtgcatga tttctttgcc agcgaacgta ttgaccaggt ctatctggcg
    10681 gcggcgaaag tgggcggcat tgttgccaac aacacctatc cggcggattt catctaccag
    10741 aacatgatga ttgagagcaa catcattcac gctgcgcatc agaacgacgt gaacaaactg
    10801 ctgtttctcg gatcgtcctg tatctacccg aaactggcaa aacagccgat ggcagaaagt
    10861 gagttattgc agggtacgct ggagccgacc aacgagcctt acgccattgc caaaatcgcc
    10921 gggatcaaac tgtgcgaatc ttacaaccgc cagtacggac gcgattaccg ctcggtcatg
    10981 ccgaccaacc tgtacgggcc gcacgacaac ttccatccga gtaactcgca tgtgatccca
    11041 gcattgctgc gtcgcttcca cgaggcgacg gcacagaatg cgccggacgt ggtggtctgg
    11101 ggcagcggta cgccgatgcg cgaatttctg cacgtcgatg atatggcggc ggcgagcatt
    11161 catgtgatgg agctggcgca cgaagtctgg ctggagaaca cccagccgat gctgtcgcac
    11221 attaacgtcg gcacgggcgt tgactgcact atccgcgagc tggcgcaaac catcgccaaa
    11281 gtggtgggtt acaaaggtcg ggtggttttt gatgccagca aaccggatgg tacgccgcgc
    11341 aaactgctgg atgtgacgcg cctgcatcag cttggctggt atcacgaaat ctcactggaa
    11401 gcggggctcg ccagcactta ccagtggttc cttgagaatc aagaccgctt tcggggggaa
    11461 tgatgttttt acgtcaggaa gactttgcca ctgtagtgcg ctccacaccg cttgtctctc
    11521 tcgactttat tgtcgagaac agtcgcggcg agtttctgct tggcaaaaga accaaccgcc
    11581 cggcgcaggg ttactggttt gtgccgggag ggcgcgtgca gaaagacgaa acgctggaag
    11641 ccgcatttga gcggctgacg atggcggaac tggggctgcg tctgccgata acagcaggcc
    11701 agttttacgg tgtctggcag cacttttatg acgataactt ctccggcacg gatttcacca
    11761 ctcactatgt ggtgctcggt tttcgcttca gagtagcgga agaagagctg ttactgccgg
    11821 atgagcagca tgacgattac cgctggctga cgccggacac gctgctcgcc agtaacgatg
    11881 ttcacgctaa cagccgcgcc tattttctcg ctgagaagcg tgccggagta cccggattat
    11941 gaaaatactg gtctacggca ttaactactc gccggagtta accggcatcg gcaaatacac
    12001 cggcgagatg gtggaatggc tggcggcaca aggtcatgag gtgcgggtca ttaccgcacc
    12061 gccttactac ccgcaatggc aggtgggtga gaactattcc gcctggtgct acaaacgaga
    12121 agagggggcc gccacggtgt ggcgctgccc gctgtacgtg ccaaaacagc cgagcaccct
    12181 gaaacgcctg ttacatctcg gcagttttgc cgtcagcagt ttctttccac tgatggcgca
    12241 acgtcgctgg aagccggatc gcattattgg cgtagtgcca acgctgtttt gcacgccggg
    12301 aatgcgcctg ctggcgaagc tctctggtgc gcgtaccgtg ctgcatattc aggattacga
    12361 agtggacgcc atgctggggc tgggccttgc cggaaaaggc aaaggcggca aagtggcaca
    12421 gctggcaacg gcgttcgaac gtagcggact gcataacgtc gataacgttt ccacgatttc
    12481 gcgttcgatg atgaataaag ccatcgaaaa aggcgtggcg gcggataacg tcatcttctt
    12541 ccccaactgg tcggaaattg cccgttttca gcatgttgca gacgccgatg ttgatgccct
    12601 tcgtaaccag cttggcctgc cggataacaa aaaaatcatt ctttactccg gcaatattgg
    12661 tgaaaagcag gggctggaaa acgttattga agctgccgat cgtctgcgcg atgaaccgct
    12721 gattttcgcc attgtcgggc agggcggcgg caaagcgcgg ctggaaaaaa tggcgcagca
    12781 gcgtggactg cgcaacatgc aatttttccc gctgcaatcg tatgacgctt tacccgcact
    12841 gctgaagatg ggcgattgcc atctggtggt gcaaaaacgc ggcgcggcag atgccgtatt
    12901 gccgtcgaaa ctgaccaata ttctggcggt aggcggtaac gcggtgatta ccgcagaggc
    12961 ccacacagaa ctggggcagc tttgcgaaac ctttccgggc attgcggttt gcgtagaacc
    13021 ggaatcggtc gaggcgctgg tggcggggat ccgtcaggcg ctcctgctgc ccaaacacaa
    13081 cacggtggca cgtgaatatg ccgaacgcac actcaataaa gagaacgtgt tacgtcaatt
    13141 tataaatgat attcggggat aattatggcg cagtcgaaac tctatccagt tgtgatggca
    13201 ggtggctccg gtagccgctt atggccgctt tcccgcgtac tttaccccaa gcagttttta
    13261 tgcctgaaag gcgatctcac catgctgcaa accaccatct gccgcctgaa cggcgtggag
    13321 tgcgaaagcc cggtggtgat ttgcaatgag cagcaccgct ttattgtcgc ggaacagctg
    13381 cgtcaactga acaaactcac cgagaacatt attctcgaac cggcagggcg taacactgca
    13441 cctgccattg cgctggcggc gctggcggca aaacgtcata gcccggagag cgacccgtta
    13501 atgctggtat tggcggcgga tcatgtgatt gccgatgaag acgcgttccg tgccgccgtg
    13561 cgtaatgcca tgccgtatgc cgaagcgggc aagctggtga ccttcggcat tgtgccggat
    13621 ctacctgaaa ccggatatgg ctatattcgt cgcggtgaag tgtcggcggg tgagcaggat
    13681 acggtggcct ttgaagtggc gcagtttgtc gaaaaaccga atctggaaac cgcgcaggcc
    13741 tatgtggcaa gcggcgaata ttactggaac agcggtatgt tcctgttccg tgccggacgc
    13801 tatctcgaag aactgaaaaa atatcgcccg gatattctcg atgcctgtga aaaagcgatg
    13861 agcgccgtcg atccggatct cgattttatt cgtgtggatg aagaggcgtt tctggcctgc
    13921 ccggaagagt cggtggatta cgcggtcatg gaacgcacgg cagatgccgt tgtggtgcca
    13981 atggatgcgg gctggagcga tgtcggttct tggtcttcat tatgggagat cagcgcccac
    14041 accgccgagg gcaacgtttg tcacggcgat gtgattaatc ataaaactga aaacagctat
    14101 gtgtacgccg aatctggcct ggtcaccacc gtcggggtga aagatctggt ggtggtgcag
    14161 accaaagatg cggtgctgat tgccgaccgt aacgcggtgc aggatgtgaa aaaagtggtc
    14221 gaacagatca aagccgatgg tcgccatgag catcgggtgc atcgcgaagt gtatcgtccg
    14281 tggggcaaat atgactctat cgacgcgggc gaccgctacc aggtgaaacg catcaccgtg
    14341 aaaccgggcg aaggcttgtc ggtacagatg catcatcacc gcgcggaaca ctgggtggtt
    14401 gtcgcgggaa cggcaaaagt cactatcgac ggtgatatca aactgcttgg tgaaaacgag
    14461 tccatttata ttccgctggg ggcgacgcat tgcctggaaa acccggggaa aattccgctc
    14521 gatttaattg aagtgcgctc cggctcttat ctcgaagagg atgatgtggt gcgcttcgcg
    14581 gatcgctacg gacgggtgta gcagttgaat aatcaaccgg attaattctc cggtaaataa
    14641 cgacaaaaac aaaattggcc gtttcggtca gggccaacta ttgcctgaaa aagggtaacg
    14701 atatgaaaaa attaacctgc tttaaagcct atgatattcg cgggaaatta ggcgaagaac
    14761 tgaatgaaga tattgcctgg cgtattggtc gtgcctatgg cgaatttctc aaaccgaaaa
    14821 ccattgtgtt aggcggtgat atccgcctca ccagcgaaac cttaaaactg gcgctggcga
    14881 aaggtttaca ggatgcgggc gtcgatgtgc tggatattgg tatgtccggt accgaagaaa
    14941 tctatttcgc cacgttccat ctcggcgtgg atggcggcat tgaagttacc gccagccata
    15001 acccgatgga ttataacggc atgaaactgg tgcgcgaggg ggctcgcccg atcagcggtg
    15061 ataccggact gcgcgacgtc cagcgtctgg ctgaagccaa cgactttcct cccgtcgatg
    15121 aaaccaaacg cggtcgctat cagcaaatca atctgcgtga cgcttacgtt gatcatctgt
    15181 tcggttatat caacgtcaaa atgcttacgc cgctgaagct ggtgatcaac tccgggaacg
    15241 gcgcagcggg tccggtagtg gacgccattg aagcccgctt taaagccctc agcgcgcccg
    15301 tggaattaat caaagtacac aacacaccgg acggcaattt ccccaacggt attcctaacc
    15361 cgctgctgcc tgaatgccgc gacgacaccc gcaatgcggt catcaaacac ggcgcggata
    15421 tgggcattgc ctttgatggc gattttgacc gctgtttcct gtttgacgaa aaagggcagt
    15481 ttatcgaggg ctactacatt gtcggcctgc tggcagaagc gttcctcgaa aaaaatcccg
    15541 gcgcgaagat catccacgat ccgcgtctct cttggaacac cgttgatgtg gtgactgctg
    15601 ctggcggcat gccggtaatg tcgaaaaccg gacacgcctt tattaaagaa cggatgcgca
    15661 aggaagacgc catctacggt ggcgaaatga gcgcccacca ttacttccgt gatttcgctt
    15721 actgcgacag cggcatgatc ccgtggctgc tggtcgccga actggtgtgc ctgaaagaga
    15781 aaacgctggg cgaactggtg cgcgaccgga tggcggcgtt tccggcaagc ggtgagatca
    15841 acagcaaact ggcgcacccc attgaggcga ttaaccgcgt cgaacagcat tttagccgtg
    15901 aggcgctggc ggtggatcgc accgatggca tcagcatgac ctttgccgac tggcgcttta
    15961 acctgcgctc ctccaacacc gaaccggtgg tgcggttgaa tgtggaatcg cgcggtgatg
    16021 taccgctgat ggaagcgcga acgcgaactc tgctgacgtt gctgaacgag taatgtcgga
    16081 tcttccctta ccccattgcg ggtaaggggc taataacagg aacaacgatg acaaatctaa
    16141 aaaagcgcga gcgagcgaaa accaatgcat cgttaatctc tatggtgcaa cgcttttcag
    16201 atatcaccat catgtttgcc ggactatggc tggtttgcga agtgagcgga ctgtcattcc
    16261 tctacatgca cctgttggtg gcgctgatta cgctggtggt gttccagatg ctgggcggca
    16321 tcaccgattt ttatcgctca tggcgcggtg ttcgggcagc gacagaattt gccctgctgc
    16381 tgcaaaactg gaccttaagc gtgattttta gcgccggact ggtggcgttc aacaatgatt
    16441 tcgacacgca actgaaaatc tggctggcgt ggtatgggct gaccagcatc ggactggtgg
    16501 tttgccgttc gtgtattcgc attggggcgg gctggctgcg taatcatggc tataacaagc
    16561 gcatggtcgc cgtagcgggg gatttagccg ccggacaaat gctgatggaa agcttccgta
    16621 atcagccgtg gttagggttt gaagtggtgg gcgtttacca cgacccaaaa ccgggtggcg
    16681 tttctaacga ctgggcgggc aatctgcaac agctggtaga ggatgcaaaa gcgggcaaga
    16741 ttcataacgt ctatatcgcg atgcagatgt gcgacggcgc tcgagtgaaa aaactggtcc
    16801 atcaactggc ggacaccacc tgttcggtgc tgctgatccc cgatgtcttt accttcaata
    16861 ttctccattc acgtctcgaa gagatgaacg gcgttccggt ggtgccgctg tatgacacgc
    16921 cactttccgg ggtaaaccgc ctgctcaaac gtgcggaaga tattgtgctg gcaacgctta
    16981 ttctgctgct gatctccccg gtgctgtgct gtattgcgct ggcggtaaaa ctcagttcac
    17041 cagggccggt tatttttcgc cagactcgct acggcatgga tggcaaaccg atcaaagtgt
    17101 ggaagttccg ttccatgaaa gtgatggaga acgacaaagt ggtgactcag gcgacgcaga
    17161 acgatccgcg cgtcaccaaa gtggggaact ttctgcgccg cacctcgctg gacgaattac
    17221 cgcagtttat caatgtgttg actggcggga tgtcgattgt cgggccacgt ccgcacgcgg
    17281 tggcgcataa cgaacagtat cgccagctca ttgaaggcta catgctgcgc cataaagtga
    17341 aaccgggcat taccggctgg gcgcagatta atggctggcg tggcgaaacc gacacgctgg
    17401 agaaaatgga aaaacgcgtc gagttcgacc ttgagtacat ccgcgaatgg agtgtctggt
    17461 tcgatatcaa aatcgttttc ctgacggtat tcaaaggctt cgttaacaaa gcggcatatt
    17521 gatatgagct tacgtgaaaa aaccatcagc ggcgcgaagt ggtcggcgat tgccacggtg
    17581 atcatcattg gcctcgggtt ggtgcagatg accgtgctgg cgcggattat cgacaaccac
    17641 cagttcggcc tgcttaccgt gtctctggtg attatcgcgc tggcagatac gctttctgac
    17701 ttcggtatcg ctaactcgat tattcagcga aaagaaatca gccaccttga actcaccacg
    17761 ttgtactggc tgaacgtcgg gctggggatc gtggtgtgcg tggcggtgtt tttgttgagt
    17821 gatctcatcg gcgacatgct gaataacccg gacctggcac cgttgattaa aacattgtcg
    17881 ctggcgtttg tggtgatccc ccacgggcaa cagttccgcg cgttgatgca gaaggagctg
    17941 gagttcaaca aaatcggcat gatcgaaacc agcgcggtgc tggcgggctt cacttttact
    18001 gtggttagcg cccatttctg gccgctggcg atgactgcga tcctcggtta tctggtcaat
    18061 agtgcggtaa gaacgctgct gtttggctac tttggccgca aaatttatcg ctccggtctg
    18121 cacttctcgc tggcgtcggt agcaccgaac ttacgttttg gtgcctggct gacggcggac
    18181 agcatcatca actatctcaa caccaacctt tcaacgctgg tgctggcgcg tattctcggt
    18241 gcgggcgtgg cagggggata caacctggcg tacaacgtgg ccgttgtgcc gccgatgaaa
    18301 ctgaacccca tcatcacgcg cgtgttgttt ccggcatttg ccaaaattca ggacgacacc
    18361 gaaaagctgc gcgtcaactt ctacaagctg ctgtcggtgg tggggattat caactttccg
    18421 gcgctgctgg ggctgatggt ggtgtcgaat aactttgtac cgctggtctt tggtgagaag
    18481 tggaacagca ttattccggt gctgcaattg ctgtgtgtgg tggggctgct gcgctcagtg
    18541 gggaacccga ttggttcgct gctgatggcg aaagcgcggg tcgatatcag ctttaaattc
    18601 aacgtattca aaacctttct gtttattccg gcgattgtta ttggtgggca gatggcgggc
    18661 gcaatcggcg tcacgctcgg cttcctgctg gtgcagatta tcaacaccat tctgagctat
    18721 ttcgtgatga ttaaaccggt gctcggttcc agttatcgcc agtacatcct gagtttatgg
    18781 ctgccgtttt atctctcgct gccgacgctg gtggttagtt atgcgttggg cattgtactg
    18841 aaagggcaac tggcgctggg gatgctgctg gcggtgcaaa tagccgcggg cgtgctggcg
    18901 tttgtggtga tgattgtgct gtcgcgccat ccgctggtgg tggaagtgaa gcgtcagttt
    18961 tgtcgcagcg aaaaaatgaa aatgctttta cgggcggggt gaatggttgt tttcggttcc
    19021 ggcaacggtt gccggatgcg gcgtaaaacg ccttatccgt cctggggggc tgggcttgga
    19081 tcttgtaggc ctgataagac gcgttagcgt agcatcaggc actgaatgcc ggatgcggcg
    19141 tgaacgcctt atccggccta cggttctgtg ctcggatctt gtaggcctga taagacgcgt
    19201 cagcgtcgca tcaggcactg aatgccggat gcggcgtaaa acgccttatc cgtcctacgg
    19261 ctct
//
LOCUS       DAPHBJ010000080        19118 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.080, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000080 DAPHBJ010000000
VERSION     DAPHBJ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.080"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(112..2778)
                     /gene="cas3"
                     /locus_tag="RHZ76_002459"
     CDS             complement(112..2778)
                     /gene="cas3"
                     /locus_tag="RHZ76_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDV2813082.1"
                     /translation="MEPFKYICHYWGKSSKSLTKGNDIHLLIYHCLDVAAVADCWWDQ
                     SVVLQNTFCRNEMLSKQRVKAWLLFFIALHDIGKFDIRFQYKSAESWLKLNPATPSLN
                     GPSTQMCRKFNHGAAGLYWFNQDSLSEQSLGDFFSFFDAAPHPYESWFPWVEAVTGHH
                     GFILHSQDQDKSRWEMPASLASYAAQDKQAREEWISVLEALFLTPAGLSINDIPPDCS
                     SLLAGFCSLADWLGSWTTTNTFLFNEDAPSDINALRTYFQDRQQDASRVLELSGLVSN
                     KRCYEGVHALLDNGYQPRQLQVLVDALPVAPGLTVIEAPTGSGKTETALAYAWKLIDQ
                     QIADSVIFALPTQATANAMLTRMEASASHLFSSPNLILAHGNSRFNHLFQSIKSRAIT
                     EQGQEEAWVQCCQWLSQSNKKVFLGQIGVCTIDQVLISVLPVKHRFIRGLGIGRSVLI
                     VDEVHAYDTYMNGLLEAVLKAQADVGGSVILLSATLPMKQKQKLLDTYGLHTDPVENN
                     SAYPLINWRGVNGAQRFDLLAHPEQLPPRFSIQPEPIYLADMLPDLTMLERMIAAANA
                     GAQVCLICNLVDVAQVCYQRLKELNNTQVDIDLFHARFTLNDRREKENRVISDFGKNG
                     ERNVGRILVATQVVEQSLDVDFDWLITQHCPADLLFQRLGRLHRHHRKYRPAGFEIPV
                     ATILLPDGEGYGRHEHIYSNVRVMWRTQQHIEELNGASLFFPDAYRQWLDSIYDDAEM
                     DEPEWVIKGMDKFESAECEKRFKARKVLQWAEEYSLQDNDETILAVTRDGEMSLPLLP
                     YVQTSSGKQLLDGQVYEDLSYEQQYEALALNRVNVPFTWKRSFSEVVDEDGLLWLEGK
                     QNQDGWFWQGNSIVITYTRDEGMTRVIPANPK"
     gene            complement(3137..3871)
                     /gene="cysH"
                     /locus_tag="RHZ76_002460"
     CDS             complement(3137..3871)
                     /gene="cysH"
                     /locus_tag="RHZ76_002460"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDV2813083.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRIAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWESGMSEEETRFFGLKRECGLHEG"
     gene            complement(3946..5658)
                     /gene="cysI"
                     /locus_tag="RHZ76_002461"
     CDS             complement(3946..5658)
                     /gene="cysI"
                     /locus_tag="RHZ76_002461"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HDV2813084.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDKWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESQKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(5658..7457)
                     /gene="cysJ"
                     /locus_tag="RHZ76_002462"
     CDS             complement(5658..7457)
                     /gene="cysJ"
                     /locus_tag="RHZ76_002462"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HDV2813085.1"
                     /translation="MTTQVPPSALLPLNPEQLVRLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQTAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDEPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAVDEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            7776..8138
                     /gene="queD"
                     /locus_tag="RHZ76_002463"
     CDS             7776..8138
                     /gene="queD"
                     /locus_tag="RHZ76_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDV2813086.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            8216..9487
                     /locus_tag="RHZ76_002464"
     CDS             8216..9487
                     /locus_tag="RHZ76_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDV2813087.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERCITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRTWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            9478..9738
                     /locus_tag="RHZ76_002465"
     CDS             9478..9738
                     /locus_tag="RHZ76_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDV2813088.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQTAERLINACPAGLFSLTPE
                     GDLRIDYRSCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            9755..10330
                     /locus_tag="RHZ76_002466"
     CDS             9755..10330
                     /locus_tag="RHZ76_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HDV2813089.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(10478..11338)
                     /locus_tag="RHZ76_002467"
     CDS             complement(10478..11338)
                     /locus_tag="RHZ76_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDV2813090.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(11335..12114)
                     /locus_tag="RHZ76_002468"
     CDS             complement(11335..12114)
                     /locus_tag="RHZ76_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDV2813091.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVECFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(12092..13501)
                     /locus_tag="RHZ76_002469"
     CDS             complement(12092..13501)
                     /locus_tag="RHZ76_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2813092.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGHFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(13523..14977)
                     /locus_tag="RHZ76_002470"
     CDS             complement(13523..14977)
                     /locus_tag="RHZ76_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDV2813093.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(15047..15832)
                     /locus_tag="RHZ76_002471"
     CDS             complement(15047..15832)
                     /locus_tag="RHZ76_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV2813094.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYLVR"
     gene            16151..17428
                     /locus_tag="RHZ76_002472"
     CDS             16151..17428
                     /locus_tag="RHZ76_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2813095.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQIAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSTSLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLTLTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            17455..18933
                     /locus_tag="RHZ76_002473"
                     /pseudo
     CDS             17455..18933
                     /locus_tag="RHZ76_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
BASE COUNT         4664 a         4941 c         4685 g         4828 t
ORIGIN      
        1 atctcatacc gggaaattaa aagaagatgt acattgtgca ccttccctac ttaagtaggg
       61 ataaaccgtt attggtcttg ttatcgtcat tgataccaat cattcccgaa gttatttggg
      121 atttgcaggg atgactctgg tcatcccttc atctcgagtg taggtaataa caatactgtt
      181 accctgccag aaccatccat cctgattctg tttcccttcc agccaaagta acccatcttc
      241 atctactact tcagaaaaac tacgtttcca ggtgaagggt acattgacgc gattaagtgc
      301 aagcgcctca tactgctgtt cataacttag gtcctcgtag acctggccat cgagcagttg
      361 tttacctgaa gacgtttgta cataaggcaa taatggcagg ctcatttccc catccctcgt
      421 taccgcaaga atggtttcat cgttatcctg caaactatat tcttcagccc actgcagaac
      481 cttgcgagcc ttgaacctct tttcacactc agcgctttca aacttatcca tgcctttgat
      541 gacccattct ggctcatcca tttcagcatc atcgtaaatg ctatccagcc attgccggta
      601 ggcatcaggg aaaaataagg atgctccatt aagttcctca atatgttgct gcgtccgcca
      661 catgactcta acgttgctat aaatatgctc atgtcgtccg taaccctcgc catcaggcag
      721 caaaatggtg gcaacaggaa tctcaaaacc agcgggacga tatttgcgat gatggcgatg
      781 taaacgaccc aatcgttgga aaagcaaatc tgcgggacaa tgctgggtaa ttaaccaatc
      841 aaaatcaacg tcgagcgatt gttccacgac ttgcgtggcg acaagtattc gtccaacatt
      901 tcgctcccca ttttttccga aatcgctaat aacacgattc tctttttctc gacgatcgtt
      961 cagcgtaaag cgtgcatgaa acaaatctat atctacttgc gtgttattta gctcctttag
     1021 ccgttggtag catacttgtg caacgtcaac caaattgcaa ataagacaga cttgtgcacc
     1081 cgcgtttgcc gctgcgatca ttcgctctaa catcgtaagg tcaggtaaca tgtcagctaa
     1141 ataaataggt tctggctgaa tcgaaaagcg gggcgggagt tgttctggat gagctagcag
     1201 atcaaaacgt tgcgcaccat tcacacctcg ccagttaatg agtggatatg cggagttatt
     1261 ttccactgga tctgtatgca gaccataagt atccagaagt ttctgttttt gtttcattgg
     1321 tagggttgcg gaaagaagaa taacactccc tcccacatca gcctgagcct tgagcactgc
     1381 ctccagcaag ccgttcatat aggtgtcgta agcatgaact tcatcaacaa ttaaaacact
     1441 tcgaccaatt cccaaaccac ggataaagcg gtgtttaact ggcaataccg atatcaacac
     1501 ctgatcaatc gtgcaaacgc cgatttgccc aagaaacact ttcttattgc tttgtgacaa
     1561 ccactgacaa cactgaaccc acgcttcttc ttgcccctgt tcagtaatcg cgcgtgattt
     1621 tattgattga aagaggtggt taaaccgtga attgccatga gcaagaataa gatttgggga
     1681 tgaaaataag tggctcgcgc tcgcttccat tctcgtaagc atagcattcg cggtagcttg
     1741 tgttgggagg gcaaaaataa cactatccgc aatttgttga tcaataagtt tccaagcata
     1801 ggccagtgct gtttccgttt taccggagcc tgtaggtgcc tctattaccg tcagcccggg
     1861 agctactgga agagcatcaa ctaacacctg taattgtctg ggttgatagc cattgtccag
     1921 tagtgcatga acaccttcat aacatcgctt atttgataca agtccactca actccaatac
     1981 ccggctcgca tcctgctgtc ggtcctggaa atacgttctc agagcattta tgtcggaagg
     2041 cgcatcctca ttaaacagaa aggtattcgt tgtagtccag gagcctaacc agtcagcaag
     2101 cgagcaaaaa cctgctaaca gtgatgaaca atcaggtggt atatcgttta tagataaccc
     2161 cgctggcgtt aaaaataatg cttccagtac agatatccac tcctcacgag cctgtttatc
     2221 ttgcgcagca taagatgcca gagaagctgg catttcccaa cgcgacttat cttgatcctg
     2281 ggaatgtaat ataaaaccat gatgtcctgt aacggcctct acccatggaa accaggactc
     2341 ataaggatga ggagcggcat caaaaaaact gaaaaaatcc ccgagagatt gctctgaaag
     2401 tgaatcctgg ttaaaccaat acagaccggc tgcaccatga ttaaatttac ggcacatttg
     2461 tgttgatgga ccattaagtg atggcgttgc aggatttaat ttcagccaac tttctgctga
     2521 tttatattgg aatcgtatat caaactttcc aatatcatga agagcaatga aaaataacag
     2581 ccaggccttc accctctgtt ttgatagcat ttcatttcgg caaaaagtat tttgcagtac
     2641 gactgattga tcccaccagc aatctgcaac agcagcaaca tcaaggcaat gataaattaa
     2701 cagatgaata tcatttcctt tcgtcaagct ttttgaggat tttccccagt aatggcatat
     2761 atatttaaaa ggttccatta atagcctccc tgttttttta gtattatcga tgatatcagc
     2821 gggctatttt tcgatgctgc tcataaatat cgaattacag tgatatacac atgtcttgcc
     2881 tccctgcaaa gaagagacta acattcgaat tgtttgggtt cgaacgctgg cctcaggttg
     2941 atagaaatat cgcctggggc ttttgtccat ctggaacctc gcgaatgctt aacgccagac
     3001 agcctcaagc acccgacgcc attctatacc tgataattct tccagcggcg ttcacgttta
     3061 aaatcaggaa tactgctcgc aaaataaaaa cgcccccatc atttctgaca gaggcgttta
     3121 atttatccgg caatatttac ccttcgtgca atccacattc cctttttaag ccaaagaaac
     3181 gtgtttcttc ttccgacatg ccagattccc atttacgggt tgtatgggta tcaccgaccg
     3241 ataaatatcc ttcatcccat aatgggtgat atttcaggcc atgtttttgc aggtactgat
     3301 aaatagttcg gttatcccag tcgataatcg gcagcacttt aaatacgcca cgctgaattg
     3361 ccagcaccgg taaattggcg cgactgccgg attgttcacg gcgcaggcca gcaaaccagg
     3421 tttgcgcgtt aagttctttc agagcccggt tcatcggttc gactttgttg atgtcattgt
     3481 acttttcaat gccttcaacg ccctgctccc acagttttcc gtagcgtgct tcctgccagg
     3541 ctgcgctttc ggtagcacgg tacactttca ggttgagctt gagtttgtcc gttaactcgt
     3601 caataaagcg gtaggtttcc gggaacaagt aacccgtatc ggtgaggatc accggaatat
     3661 ccgggcgaat ttgattcacc agatgcaggc tcaccgccgc ctgaatgcca aagctggaag
     3721 aaagtacata ttcaccgggc agattatcca gcgcccaggc tatgcggcct tcagcgtcca
     3781 gtttttccag ttcggcgtta gtttccgcca gcgccagaat gcgatctacc ttcggcagtt
     3841 cgttcagggc gtttagatcg agtttggaca tagattcctc actgtttgcc ttgcctgatg
     3901 cgacgctcac gcgttcttat caggcctaca agaccgggct gatggttaat cccacaaatc
     3961 gcgcgccgga tcgagcaccg ggcgaatgat gcccgcacgc accgtaaagt cgccgaagcc
     4021 ttcacccgct tcgcgctctt tcgcccagcg ccctatcagt tcatcaagcg acgccaggat
     4081 ttccggctcg gtgatgtttt ctttatacat ccgtgggata cgtgtcccaa tgcggttgcc
     4141 gccaagatgc aggttgtagc gacccggcgc tttacccacc aggcccactt ccgccagcat
     4201 cgcgcgacca caaccgttcg ggcagcctgt tacacgcatc acgatatgct catcgctgac
     4261 accatgtttc gccattaaat tatcgatgtt gtcgataaaa gacggcagga aacgctccgc
     4321 ttccgccatc gccagcgggc aagtcgggaa tgacacgcag gccattgagt tttcacgctg
     4381 cggcgtgacg gcattcatta acccgctctc tttagcgatc ttctcgatct tcgctttctg
     4441 gctttcaggt acaccggcga tgatcagatt ctggttcgcc gtaatacgga aatcgccttt
     4501 gtggatcttc gcgatctcca gcaggccggt tttcagcgga cgccccggat aatcaaggat
     4561 tcggccattt tcgataaaca gcgtcaggtg ccatttgtca tcaatgcctt ttacccaacc
     4621 aatgcgatcg ccgcgccctg taaattcata cgggcggatc ggttcgaatt tgatccccgc
     4681 gcgacgttcc acttccgctt taaatgtctc aacacccacg cgctccagcg tgtatttggt
     4741 tttggcattt ttacgatcgg tacggttacc ccaatcgcgc tgagtcgtca cgacggcttc
     4801 cgccaccgcc agggtatgct ccagcggcag ataaccaaac tcgctcgctg tgcgggcgta
     4861 ggttttctta ttaccgtgtt cgatggaaag cccgccgccc accagcaggt taaagcctac
     4921 cagcttgccg ttttcggcga ttgccacgaa gttcatgtcg ttggcatgca gatcgatatc
     4981 gttctgcggc gggatcacta ccgtggtttt gaatttacgc ggcaaataag tctgaccgag
     5041 gatcggttct tcatcggtgg tggcgacttt ttcctgatcg agccagatct ccgcatacgc
     5101 gcgggtacgc ggcagcagat gttcagagat tttcttcgcc cattcatacg cttccgcgtg
     5161 tagctgcgac tcgtaagggt tcgaggtgca gagtacgtta cggttcatgt cgttagcggt
     5221 cgccagcgca tcaagaccaa ccgagtgcag catctggtgc accggtttga cgttcttttt
     5281 cagaatgccg tggaactgaa acgtctggcg gttggtcagg cgaatgctgc catagatggt
     5341 gttttcaccg gcaaatttgt cgatcgcctg ccactgttta gtggtaatca ccccacccgg
     5401 cagacgacag cgaagcagca tcgcgtggcg cggctccagc ttctgttcag cacgttcggc
     5461 gcggatgtcg cggtcatcct gctgatacat gccgtggaag cggatcagca ggaagttatc
     5521 gcccttaaag ccgccggtca ggccgtcgtt taaatcttcc gcaatggtgc cgcgcaggta
     5581 gttgctttca agcttcatgc gctcggcgtc tgtcagtttt ccttcgacca ctaaaggccc
     5641 tggatgtttt tcgctcatta gtagacatct cgctgataac ggcgctctac gcgcagttca
     5701 cttaaaaatt catccgccgc ttcggtgtcc atgccaccaa attcggcaat cacttccaga
     5761 agtgcctgct caacgtcttt cgccatgcga ttagcgtcgc cgcagacata aatgtgggca
     5821 ccgtcattga tccagcgcca cagctccgcg ccctgttcgc gtagtttgtc ttgtacgtaa
     5881 actttttctt tttgatcgcg cgaccaggcg agatcgatac gtgtcagcac gccctctttg
     5941 acgtagcgct gccactccac ctggtagagg aaatcttccg taaagtgcgg gttgccaaag
     6001 aacagccagt ttttacccgg tgcttcatcg acagcacgtt gctgcataaa ggcgcggaac
     6061 ggcgcgatac cggtgcctgg gccaatcata atcaccgggg tttctggatt ggctggtagg
     6121 cggaagttat cgttatgttc gataaatacg cggacttcgc cctcttcttc cacgcggtca
     6181 gcgaggaagc tggaggcacc accggcacgg gcgcggcctt ccacgtcgta acgcaccaca
     6241 ccaacggtga cgtgtacttc gttctcgact tccgcctgcg aggaggcaat ggaatagaga
     6301 cgcggcgtca gcgggcgcag cagattaatt agcgcttcgg catcaagctg tgccggggag
     6361 aaacgcacca tgtcaacaat cggcgtcgtc gcggcgtaat gctgtaactt cgctttatcg
     6421 cccaccagcg gcagcagtgt ttcactgcgg gtaagcgtgg cgtaattctc aacaatgttg
     6481 gcggtgttga cggtcagttc gaagtgccac tgtagcgctt cgttcagagg caacgttttg
     6541 ccctcgacgg tgacaggttc atcgcctttc agccacagca gttcgacaag ttctttcacc
     6601 agtgccggat cgttctgata ccagacgccc agcgcgtcac ccggctggta acgcaggccc
     6661 gagtcaccta agtcaatttc gatatggcga acgtcttttt cagagttacg cccggtaatt
     6721 ttctggttaa cagagaggct cgccaccagc ggctcgtctt tgctgtacgg gctggtgtgg
     6781 atttcattta ccgcgccagt agcgacggat tgcgaaggtg ccgcgacagg cgcacgcgat
     6841 ttaagcgcat caaccacacg agcgcgccac tcgctggcag cggtctggta ttcaacgtcg
     6901 gcatcgacac ggtcgagcag gcgttcaccg cccagttccg ccagcttgct gtcaaaatct
     6961 ttcccggact ggcagaaaaa ttcataagag ctatcgccga ggctaaacac ggcaaatgcg
     7021 gtgttttcca gcttcggcgc ttttttggag aacaggaact tatgcagcgc gacggcttct
     7081 tccggcggtt ccccttctcc ttgcgttgac gtcaccacga taagcagttt ttcgctggcg
     7141 atttgtttga atttatagtc gcccgcgttc accagcttaa cgttcagttt tgccgctaat
     7201 aaatcatcac gtaatgcttc agcaacccgg cgcgcattgc cggtttgcga ggcggagata
     7261 atagttatac ccggcatttc tgcggcggga gctggcgtcg ctgcaagcgc agcaggctgc
     7321 tgattgagca cgccccagaa atagccagaa acccaggcaa gctgggtggg agttaaatcg
     7381 gtcgtggccg cctgaaggcg taccagttgc tccgggttca acggaagcaa cgcggaaggt
     7441 gggacctgtg tcgtcatgcg tcgttatgtt ccagtaagca aagctgtttc tgcgccctgt
     7501 cagcgcccat aaaacagaag agaaggtaag gttaacgggg tgaacggtgt ggattaaaga
     7561 cgggatagcg ataactaata accaaatcga ctaacctgtt ttagcaatag tctttaacaa
     7621 caaaatagat taaccaacct aatgaaaaac aaatgaattt agccaatcat taagataaat
     7681 cagcgatttt gcgcaacaaa gtcgttttag ataatgcgaa aaaacagcct ttccggtact
     7741 ctacggcggt tttatcgtcc tgtagagaaa ttatgatgtc caccacgtta tttaaagatt
     7801 tcaccttcga agccgctcac cgcttaccac acgtcccgga agggcataaa tgtggtcgcc
     7861 tgcacgggca ttcctttatg gtgcgactgg aaattaccgg ggaagtcgat ccgcatacgg
     7921 gctggattat cgatttcgct gaactgaaag cagcgtttaa gccaacttat gagcgcctcg
     7981 atcaccatta tctcaatgat attccaggcc tggaaaaccc gaccagcgaa gttttagcaa
     8041 aatggatttg ggatcaggtt aaacccgttg tgccgctgtt aagtgcggtg atggtaaaag
     8101 aaacctgcac cgctggttgt atctatcgcg gcgaatgata agagtgtgtc ggcggtcaat
     8161 ttcccttaag taacgctatg ttagggtgtt gtgttctgga tatctggggc atgacatgga
     8221 agacgactgc gacattatta ttattggtgc cggtattgca ggcaccgctt gcgcgttacg
     8281 ctgcgcgcga gcgggtttat ccgttttgtt actggaacgc gctgaaatcc ccggcagcaa
     8341 aaatctttcc ggcgggcggt tatataccca tgcactcgcg gaactcctcc ctcagtttca
     8401 tctgaccgcg cctcttgaac gatgcatcac tcacgaaagc ctttccctgt taacgccgga
     8461 tggcgcaacg acgttttcca gcttacagcc cggcggtgaa tcctggagtg tattacgtgc
     8521 acggttcgat ccgtggctgg ttgccgaagc cgaaaaagaa ggtgtcgaat gcatccctgg
     8581 tgcgacggtg gatgcactgt atgaagaaaa cggcagggtg tgtggtgtca tttgtggtga
     8641 cgatattctc cgcgcccgtt atgtggtgct ggcagaaggt gccaacagcg tcctggctga
     8701 gcgtcacggg ttagtgactc gtcctgctgg cgaagcgatg gcgttgggga tcaaagaagt
     8761 gctgtcgctg gaaacatccg ctattgaaga acgttttcat ctggagaata acgaaggcgc
     8821 agcgttgctg ttcagcggcg ggatctgtga tgacttaccc ggcggcgcat ttctttatac
     8881 taatcaacaa acgctctcgt tagggattgt ttgcccgctc tcttccctta cgcaaagccg
     8941 tgttccggca agcgagctgc tgactcgctt taaagcgcat ccggcagtgc gcccgcttat
     9001 caaaaacacg gaatcactgg agtatggtgc gcatctggtg ccagaaggtg gcttgcacag
     9061 tatgccggtg caatacgccg gtaacggctg gctgctggtg ggcgatgcgt tgcgcagttg
     9121 cgtcaatacc ggaatttccg tgcgcggcat ggatatggcg ctgactggcg cgcaggcggc
     9181 ggcacaaacg ctgataagcg cctgccagca ccgcgagccg caaaatctgt ttccgcttta
     9241 tcatcacaac gtagagcgca gcctgctgtg ggatgttcta cagcgttatc agcatgttcc
     9301 ggcgcttttg caacgcccgg gatggtaccg tacgtggcct gcgttaatgc aggatatttc
     9361 ccgcgattta tgggatcagg gtgataaacc tgttccaccg ctgcgccagt tattctggca
     9421 tcatttacgt cgtcacggcc tgtggcatct ggcgggcgat gttatcagga gtctgcgatg
     9481 tctgtagccc gtaatctctg gcgcgttgct gatgcgccgc acattgttcc ggctgactcc
     9541 gttgagcgcc agacggcaga acggttgatt aacgcctgtc cggcaggtct tttttcgctc
     9601 acaccggaag gtgacttacg tattgactat cgcagttgcc tggagtgtgg cacctgccgt
     9661 ttgctgtgcg acgaatcaac actacaacag tggcgctatc cgccttccgg attcggcatc
     9721 acctaccgct ttggataaat aataaggata atttatgccc ctcttacacc tgctccgcca
     9781 gaatccggtg attgctgccg ttaaagataa tgccagcctg caactggcaa tcgattctga
     9841 atgccaattt atttccgtgt tgtacggcaa tatctgcacc atcagtaata tcgttaaaaa
     9901 gattaagaac gccgggaaat atgcttttat tcatgttgat ttgctggaag gcgcgtcaaa
     9961 taaagaagtc gttattcagt ttttgaaact ggtgaccgag gcggacggca ttatcagtac
    10021 caaagcctca atgttgaaag cggcaagagc ggaaggtttt ttctgtattc atcgcctgtt
    10081 tattgttgat tcaatttcgt ttcacaacat tgataagcaa gttgcgcaat cgaatccgga
    10141 ttgtattgag atcctgccag gctgtatgcc caaagtgctg ggctgggtga cagagaaaat
    10201 ccgccaaccg ctgattgccg gtgggctggt gtgcgatgaa gaagatgcgc gtaatgcgat
    10261 taacgcgggt gtcgtggcgc tttccaccac gaataccggg gtctggacgt tagcgaaaaa
    10321 attactttga cgggataatc gtaaccaatt gaatttggtt tgatttgtag gccgcacgcc
    10381 acatccgaca ttcagcgcct gatgcgacgt ttgacgcgtc ttatcaggcc tacaagtccc
    10441 caggccagaa ccgtaggcag gatcatccgg caacacgtta ttggcaatct gcgtgaatgt
    10501 ttgtcaccag tgcctccagc accactttcc agtcatcaac cacgccaaca tctgcctgtg
    10561 aaaataccgc agcgctggcg tcatgattaa tcgccaccac aaacttgcta ttgcgtacgc
    10621 ccgccattaa cgctgctgcg ccagaagcgc caacaacaat acacacctca ggtgccagca
    10681 gatgtccgga aataccaatc actttttcag catcaacgcc accgttcatc actcgcgccc
    10741 gactgtagcc cacttcggct cccagctttt ctgccagcat agcgatttcc tggttatctg
    10801 cctctccgcc ctgccccacc accagcaccc gtctggcctc agctaatggg tcgcgagtaa
    10861 catttttcag atcctctgtg ctaaccagcc agtccggaag cgcacccggg acaatgttca
    10921 gttgttgcat tccagagggc aacgtggcgt ttttcgctgc tcccgcctga cgcgccaggg
    10981 aaaggcatag cgggcgtttt tctgtttgca gcgtcgccgt tagcgcattt ccccagtggg
    11041 atttgcgcac gcttacggtt ggaatatcta acgaagtcac ctggcagatg cttgcaccat
    11101 gtaaacgcca ggccagccgg gtcgacagtt catcgccaaa cgttcccggc ggaaagagaa
    11161 caacgtccgc aggcgttcgc tgccactgtt ccaccaacgc atccagcacc tgttccgcca
    11221 ctacaggctg aggttcaatc tgccagtgcg ccagggtaca accgctgaaa tcctgcgccg
    11281 ccagccagct tgctattgcg gcattctcct gatttattgt cacaattgcg atattcatgg
    11341 ctgcatcctt tggcgtaaat agtcctgcca tagcttttga gctttttctg ctaccgtttg
    11401 cccatcaatc agcgtcgctc cgcgtcgttg ctctgctctt gccagttgca gacactgcat
    11461 cgctggcatt tcggcagcaa cagttttacg gataatttct gctttccccg cagccattcg
    11521 ctggcgcatt cccggtactg gcaaggccac ttcaccgcac tgacgcacgg caatcaccgc
    11581 aggcaagcga acccggcaac aacgcaaccc atgttcagta cgctgttcga gggtgataaa
    11641 cagcgcgtcg agagtgaaac actccacctg ggtgaagcag ggccagccca gcatttccgc
    11701 cagcaaaaat ggcgtttgcc cattctgccc ttcgctgctt tggcagccgg taattatcag
    11761 atccagcgga ttctgatgct gccattcggc aatatggcga gcgacaaatt ccggcgcaaa
    11821 gcgcagatct gccgccgtct ccagcaatac agcttcctca aacccaagag ccatgagata
    11881 gcgcagccag tgcaatgccc gttcatcccc catgctcaac gcggttaaag acatcggcgt
    11941 accgtttttt ctctgcgcca acagcagcgc ggcagcggcc tgttcatcag caccgagtaa
    12001 acttcgcagt agcgaaatat ccggtccgct tttaccctga gccgccgcct gccactcttt
    12061 ttccgccagc attccggcat ccggttcggc tttaaacgct aacagaatgt tcattcgcac
    12121 ctcctacatt tcccgccgcc accagcggga gggctttagt ttctggtgcc catagccagg
    12181 tcacaacaaa accaaccaac aacactgtcg ccaggagcaa taaggtgact tgcattcccc
    12241 actgcgccag cacccacggc agcaggccag tacttaccgc cgcgccaagt cgactcatgg
    12301 cggtggcaaa accgacgccc agcgagcgaa tgtcagtagg aaaactttcc gcaggcaaaa
    12361 tgcccaccag attactgact gccgaaatgg tggtgctgaa gagaacaaaa agcagcagcg
    12421 ttaatgaact gccggaaggc aaacaggcca ttactaccag cgttgccgcc agcagcaaaa
    12481 aacttcccag caaaaatttg cgatgtgcca gcaggtgcgt cagaactaat cccagcagcg
    12541 cgcccacaat taacaacgca ttaagcatca ggctggcagt cagcgcatct tccagaccaa
    12601 tagtctgggc gatagttggc agccaggtat aaatcacaaa ccatgggatt acgaggcaga
    12661 caaagaagac gctgttaaac gccgtgcgcc gccagtaacg cgaagagaac aaggttttga
    12721 tgtgtttatg ggtcgccgtt accacttcat cgcccagtaa aacatgggga ccaaaatagc
    12781 gatgcacgat agcgtgagct tctgcaaaat gcccctggcg cagtagccag cgtggtgatt
    12841 ctggcgttcc ccagcgtaat aacgtaatca acaacgcggg cagagctgcc gatgccagta
    12901 gccagcgcca ggcctccggg ttttcggaaa taaagtgatg tccggcaata cttgccagca
    12961 catagcctac ggtccacacc acgctgaatg cgcccagcaa aataccgcga tggcggcgcg
    13021 gggaaaattc agccagcaag gtgtgaccta ctgaataatc gcctcccaga ccaatgccaa
    13081 tcaaaatgcg cagtccaata agatgctctg gcgtggtggc aaaaaattgt aaaaacgaag
    13141 caagcgtaat cagcaaaaag ctgaaggtga agattttttg ccgaccaata tggtcggaga
    13201 tccacccaag aaccaggctg ccaaggaaca aaccaaggag cgccgagccg ccgatcattc
    13261 ccgccataaa cggcgtcagt tgcatggcgg gcgtaagctg aataatggcg taaccaatga
    13321 cgccgagaac ataaccgtcg gtcaggtgtg cgccgaaagt gagcgcagca atgcggcagt
    13381 gaaaacggtt aagcggtaaa tcatccattc gcaccggtga agtgttcatc tttacccctt
    13441 cattgtccgg taataagaac ggcttctcgc ctgagaagcc gaaaaggcaa cgccccgtca
    13501 tcgcggaacg ctgcctgaca cattattttt caatcggata gatagtaccc gtattcataa
    13561 tgccattagg atcgaactgc tttttcagcc cttccagcaa cgcccacgcg ctgccgtgtt
    13621 ccagtttgct ccagtgaacg cgatgtttac cgataccgtg gtggtgcacc atcgaaccgc
    13681 cgaggcgaat ggtttcttca cagatgatct tgttgagcgg attgtggtac ttgtcgattt
    13741 cctcttccgg cttacagtca acgacgttgt aatcgtagac gaagtacatg ttggtgccgt
    13801 tctgatagct atgagaggag tgaccgccca gcatggtgat gtcgtcggcg tgcgggaact
    13861 cagtacgaat acggttaata acgctttcgt agatttcgtg gatgcagctc cagcagccgg
    13921 acacttcagt ggtaaagccc atgttgccgg ttttgaggat ctgcacacgt tcggcagcca
    13981 ctttatccgg tccccagttc aggttgttaa accaggtttc gatcagcttg ctgtccacgc
    14041 gctggcattg cgggtagcgg gcaacgattt ccgcaatccc ttcgcccgtc gccttcgcaa
    14101 tgcgagggtt accttcagcc ataaagatca gcacgcattt tccgtcggca aaatgggtga
    14161 agtgttgggt gccatcttca gcgtcataca aacgagcgat cgacggacga tacccttcca
    14221 ccatgatttc acgcaggatg ttgaagccgg ttttcatgtc ttccaggata tagccgtaga
    14281 agaggttgtt ttccggggtg aatttaaaga ttttcactgt tacttcagtg atatagcaaa
    14341 gtgcaccttc gttaccgatg atgatgtggc gaatgtccgg gccagccgcg cggcgtggca
    14401 cgtttttaat acgtgtcacg gtgccatcag ccaatactgc ttccagacca acgaccatat
    14461 cttcgattgc gccgtagagt gtggagaact gcccgatact gcgggttgct accaggccgc
    14521 ccatctgcgc cagcggcttt gactgcggag aatgccccgt ggtgtaacct ttttcacgca
    14581 acgcgttttc cagcacttcc agcggaacac cacattgcgc cgtggcctgc atattctcaa
    14641 tatcaatatt aatgatttga ttcatggcgg agccgtcgag caccaccgag ttttccacaa
    14701 cggtttccag cccaccttcg gtagcggaag caccggtacg cggcacaccg ttaattttgt
    14761 gcgcattcat aaaattcagc acacgggaga cttgttctgt ggaaccgagt tttacgaccg
    14821 ctgccggaat cggcaaagta taaatgccat gaatatccgg aaatttacga aaacggtcaa
    14881 tactgttctt ctttaatact gtttcatcgg taattacgcg atctgcacca acaatttcct
    14941 ttagctggtc gacaatcgct gcgcgagata aagacatagt aattccttcc tgataataaa
    15001 aatggaaaaa tcattaataa gagaaaacag aaaccaccgt tatggattag cgcactaaat
    15061 aaccgccatc gaccactaat aaatgcccat tgacataatt cgatgcctga cttgcgagaa
    15121 acacggctgc acccattaaa tcctgagtat cgccccaacg gtttgccgga atatgatcaa
    15181 gaacgcgctg attggtttct ggattactgc gtgtcgccag cgtaatatct gttgcataat
    15241 agccaggggc gataccattt acctgaatat tatattgacc taattcatca caataagctt
    15301 tggtgaaccc ggcaagagca tgtttagtgg cagaatatgc gggtgaccat tgtccaccta
    15361 agtaagagaa caatgaacag atattaataa ttttaccgct tttctgcggg atcataattt
    15421 ttgccgcttc atagcttaac tcgaatgcgg cggtcaggtt cacatcaatc atcggatccc
    15481 agtcggcacg accgaagtcc agcaccttat tcagcttaca aataccggca ttgtttacca
    15541 gaatatcaac tgtaccgaaa cgctcacagc aagcagcgat aatcttctgc ggcgcgcctt
    15601 ctgcggtgat atccacctgc atgaagtcca cctcaacacc ctgtttttca atcatttcct
    15661 ttgtttcgcc gttatctttg acgaaactag gaataaagat atttgcgcca gctttggcca
    15721 acgccatggc aaatgcctgg cctaaaccgc tattcccacc ggtaacaatt gcggttttac
    15781 ctttcagtga gaaaaaatcc attgagaacg cattgagaga ttcgattgac atagttagct
    15841 ccaatttccg taaggcaaaa aaaaagaaag gtaacttccc ccgcgagagg gaagttacct
    15901 ttctcattat ctctggtaac tggagataat ttttagcata acggcataac tgctaatgtg
    15961 atcgtaatca cagtgtgata ttcgtattgt caaatagttt taaaaagcag aagcgtgatt
    16021 actctcacac aacacctctg ctttccatgt tatttattat ttcaattact agagacctga
    16081 gaaaagtaat ccacctgaaa gggcggctta cttttctctt ttttttgtct attacaaatg
    16141 ggaatacgat atgcaacaca actcatatcg ccgttggata accctcgcga taattagttt
    16201 tagcggcggc gttagtttcg acctggctta tttacgttat atttatcaaa ttcccatggc
    16261 gaaatttatg ggattcagca ataccgagat aggtttaata atgagtacct ttggtattgc
    16321 tgctattatt ctttatgccc ccagcggcgt tattgccgat aaattttcac accgcaaaat
    16381 gattacttcc gcgatgatca ttaccggatt actgggtctg ttaatggcaa cgtatccacc
    16441 gctgtgggta atgctctgta ttcagatcgc ctttgcgata acgacgattt taatgctgtg
    16501 gtcggtgtcg attaaagctg catcgttgct tggcgatcat agcgagcaag ggaaaattat
    16561 gggctggatg gaagggctgc gcggcgtcgg tgtaatgtcg ctggcggtgt ttaccatgtg
    16621 ggtcttttct cgctttgcac cggatgacag caccagcctg aaaacggtca ttatcatcta
    16681 tagtgtggtt tacatcttgt tggggattct gtgctggttt tttgttagcg ataacaacaa
    16741 cctgcgcagt gccaataacg aagaaaaaca gtcattccag cttagcgaca tcctggccgt
    16801 tttgcgtatc agcaccacct ggtattgcag catggtgatt tttggcgtct tcaccatcta
    16861 cgccattctg agttactcta ccaactatct gaccgaaatg tatggcatgt cgctggtggc
    16921 ggcgagctac atggggattg tgatcaacaa aatattccgc gcgctgtgcg gcccacttgg
    16981 cggcataatc accacctaca gcaaagtgaa atcccctacc cgcgtgatcc aaatcctttc
    17041 cgtactcggc ctgctgacgt taactgccct gctcgtcacg aactctaacc cgcaatcggt
    17101 cgcgatgggg attggcctga ttttactgct gggattcacc tgttacgcct cacgcgggct
    17161 gtactgggcc tgccctggcg aagcgagaac accgtcttac attatgggca ccacggtagg
    17221 tatttgttcg gtgattggat tcctgccgga tgtcttcgtt tacccaatta tcggccactg
    17281 gcaagacacc ctgcccgctg cagaagccta ccgcaatatg tggctgatgg gcatggcggc
    17341 gctcggcatg gtgattgtct ttaccttttt gctgttccaa aaaattcgta ctgctgatag
    17401 cgcccccgca atggctagca gcaagtaagc caggaaaatt acaggagaaa aatcatgtcg
    17461 aaaaaataca tcatagggat tgatggcgga agtcagagca caaaagtggt gatgtacgat
    17521 ctggaaggta atgtggtttg cgaaggtaaa ggcttattac agccgatgca cacgccagat
    17581 gccgatactg cagaacatcc tgacgacgat ttatgggcat cattatgttt tgccggtcac
    17641 gatttgatga gtcagtttgc cgggaataaa gaagatattg tcggtattgg tctgggatcc
    17701 atccgttgct gccgtgcgtt attgaaagcc gatggcacgc cagctgcgcc gttgattagc
    17761 tggcaggatg cacgcgttac acgcccttac gaacatacga atcctgacgt ggcgtatgtc
    17821 acctcttttt cgggttatct gacgcatcgc ttaaccggcg agtttaaaga caatatcgcc
    17881 aactattttg gtcagtggcc ggtggattat aagagctggg catggagcga agatgctgcg
    17941 gtaatggata agtttaatat cccccgtcat atgctgtttg atgtgcaaat gcctggcacc
    18001 gtcctcggac atatcacacc acaagccgca ctggcgacac atttcccggc aggactgccg
    18061 gttgtttgta ccaccagtga taaaccggta gaagctctgg gggctggatt actggatgat
    18121 gaaactgcgg taatttctct tggcacttac atcgcattga tgatgaacgg caaagcactg
    18181 ccgaaagatc cggtggcgta ctggccgatt atgtcttcta ttccgcaaac attgctgtat
    18241 gaaggttacg gtattcgcaa aggtatgtgg acggtgagct ggctgcgtga catgttaggc
    18301 gagtcgttaa ttcaggatgc cagggcgcag gatctttcac cggaagattt actcaacaag
    18361 aaagcctcta gtgtgccacc tggctgtaat gggctgatga cggtgctgga ctggctgacc
    18421 aatccgtggg aaccgtacaa acgcgggatt atgatcggct ttgattccag catggattac
    18481 gcatagatat atcgttcgat attggaaagt gtggcgctaa cgctgaagaa caattacgac
    18541 aatatgtgta atgaaatgaa tcactttgcg aagcatgtga tcattactgg cggcggttcg
    18601 aacagcgatc tgtttatgca aatttttgcc gacgtgttca accttccggc acgccgtaac
    18661 gccattaacg gttgtgcaag tctgggagca gcgattaata cagcggtagg tctggggcta
    18721 tacccggatt acgcaacggc tgttgataag atggttcgcg tgaaagatat ctttataccg
    18781 attgagagca atgccaaacg ctacgacgcg atgaataaag gcattttcaa agacctaacc
    18841 aaacatactg atgtgatcct gaaaaaatcg tatgaagtga tgcatgggga attggggaat
    18901 gtggattcga tccagagctg gtcgaatgcg taagtgagga aggccgggcg ggaaactgcc
    18961 cggcctgaac atacctgaat ggttatcccc gctaacgcgg ggaacataag ttcgacaact
    19021 taaaaaatga taacaagtcc ggtttatccc cgctgatgca gggaacataa taaaaacttt
    19081 aactagttcg gtttatccct gtttttacaa ggaatatt
//
LOCUS       DAPHBJ010000081        19012 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.081, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000081 DAPHBJ010000000
VERSION     DAPHBJ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.081"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            52..657
                     /gene="hyfA"
                     /locus_tag="RHZ76_002474"
     CDS             52..657
                     /gene="hyfA"
                     /locus_tag="RHZ76_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HDV2813096.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVLPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLT"
     gene            669..2686
                     /gene="hyfB"
                     /locus_tag="RHZ76_002475"
                     /pseudo
     CDS             669..2686
                     /gene="hyfB"
                     /locus_tag="RHZ76_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            2697..3644
                     /gene="hyfC"
                     /locus_tag="RHZ76_002476"
     CDS             2697..3644
                     /gene="hyfC"
                     /locus_tag="RHZ76_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HDV2813097.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRR
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLICLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLELKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            3661..3771
                     /locus_tag="RHZ76_002477"
     CDS             3661..3771
                     /locus_tag="RHZ76_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HDV2813098.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            3747..5099
                     /gene="hyfD"
                     /locus_tag="RHZ76_002478"
     CDS             3747..5099
                     /gene="hyfD"
                     /locus_tag="RHZ76_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HDV2813099.1"
                     /translation="MGVLFAALTTLCMLSLISTFYQADKVAVTLTLVNVGDVALFGLV
                     IDRVSTLILFVVVFLGLLVTIYSTGYLTDKNREHPHNGTNRYYAFLLVFIGAMAGLVL
                     SSTLLGQLLFFEITGGCSWALISYYQSDKAQRSALKALLITHIGSLGLYLAAATLFLQ
                     TGTFALSAMSELHGDARYLVYGGILFAAWGKSAQLPMQAWLPDAMEAPTPISAYLHAA
                     SMVKVGVYIFARAIIDGGNIPHVIGGVGMVMALVTILYGFLMYLPQQDMKRLLAWSTI
                     TQLGWMFFGLSLSIFGSRLALEGSIAYIVNHAFAKSLFFLVAGALSYSCGTRLLPRLR
                     GVLHTLPLPGVGFCVAALAITGVPPFNGFFSKFPLFAAGFALSVEYWILLPAMILLMI
                     ESVASFAWFIRWFGRVVPGKPSEAVADAAPLPGSMRLVLIVLIVMSLISSVIAATWLQ
                     "
     gene            5111..5761
                     /gene="hyfE"
                     /locus_tag="RHZ76_002479"
     CDS             5111..5761
                     /gene="hyfE"
                     /locus_tag="RHZ76_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HDV2813100.1"
                     /translation="MTGSTIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            5766..6395
                     /locus_tag="RHZ76_002480"
     CDS             5766..6395
                     /locus_tag="RHZ76_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HDV2813101.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGHSATRAVTVLHSLGIT
                     LLLILALWLVQTAAGAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQ"
     gene            6399..7346
                     /locus_tag="RHZ76_002481"
     CDS             6399..7346
                     /locus_tag="RHZ76_002481"
                     /inference="COORDINATES: protein motif:HMM:NF012581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813102.1"
                     /translation="MLDPTLMLLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSA
                     LLSAVLLNCALLVMIRYYIIICQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRL
                     LAYSSVENMGLVAVALGIGGPLGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLN
                     VVCGMLKIMPFTAVLFGGGALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVMLLLLL
                     TLVLAGLVRMAARVLMAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRIL
                     AGASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER"
     gene            7336..9051
                     /gene="hyfG"
                     /locus_tag="RHZ76_002482"
     CDS             7336..9051
                     /gene="hyfG"
                     /locus_tag="RHZ76_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HDV2813103.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDIVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSDAQVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTISDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            9061..9606
                     /gene="hyfH"
                     /locus_tag="RHZ76_002483"
     CDS             9061..9606
                     /gene="hyfH"
                     /locus_tag="RHZ76_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HDV2813104.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            9603..10361
                     /gene="hyfI"
                     /locus_tag="RHZ76_002484"
     CDS             9603..10361
                     /gene="hyfI"
                     /locus_tag="RHZ76_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HDV2813105.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLREADDPRLNSIVQQLFRVLRGLHD"
     gene            10354..10767
                     /gene="hyfJ"
                     /locus_tag="RHZ76_002485"
     CDS             10354..10767
                     /gene="hyfJ"
                     /locus_tag="RHZ76_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HDV2813106.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DNATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            10797..12809
                     /gene="hyfR"
                     /locus_tag="RHZ76_002486"
     CDS             10797..12809
                     /gene="hyfR"
                     /locus_tag="RHZ76_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HDV2813107.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTSQDCTLFNWLARIITPVLQSWLNDEEQQVAQRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIECLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            12831..13680
                     /locus_tag="RHZ76_002487"
                     /pseudo
     CDS             12831..13680
                     /locus_tag="RHZ76_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(13718..14779)
                     /locus_tag="RHZ76_002488"
     CDS             complement(13718..14779)
                     /locus_tag="RHZ76_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDV2813108.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            14992..16455
                     /gene="bepA"
                     /locus_tag="RHZ76_002489"
     CDS             14992..16455
                     /gene="bepA"
                     /locus_tag="RHZ76_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HDV2813109.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            16476..16835
                     /gene="arsC"
                     /locus_tag="RHZ76_002490"
     CDS             16476..16835
                     /gene="arsC"
                     /locus_tag="RHZ76_002490"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HDV2813110.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(16973..17719)
                     /gene="hda"
                     /locus_tag="RHZ76_002491"
     CDS             complement(16973..17719)
                     /gene="hda"
                     /locus_tag="RHZ76_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HDV2813111.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(17769..>19012)
                     /gene="uraA"
                     /locus_tag="RHZ76_002492"
     CDS             complement(17769..>19012)
                     /gene="uraA"
                     /locus_tag="RHZ76_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDV2813112.1"
                     /translation="TIPLSLQHLFAMFGATVLVPVLFHINPATVLLFNGIGTLLYLFI
                     CKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFCLVSFIVKKAGTGWLDV
                     LFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTIIISITTLAVTVLGSVLF
                     RGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTLYTPRFEWFAILTILPA
                     ALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVISGFFGSTPNTTYGENI
                     GVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVMGGVSLLLYGVIGASGI
                     RVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGMALATIVGIGLSLIFKL
                     ISVLRPEEVVLDAEDADITDK"
BASE COUNT         4193 a         5078 c         5128 g         4613 t
ORIGIN      
        1 cctttatgca atatgaaatg caatgtttca tatcattttc aaggagccga catgaaccgc
       61 tttgtggtgg ccgaaccact gtggtgtaca ggatgtaata cctgtctcgc tgcctgttcg
      121 gacgtgcata aaacgcaagg tttacagcaa cacccgcgcc tggccctggc gaagacgtca
      181 acaatcactg cccctgtcgt gtgtcatcac tgtgaggaag ccccttgcct gcaggtctgc
      241 ccggtcaatg ccatctctca gagggatgat gcgatccaac tcaacgaaag cctctgtatt
      301 ggctgcaagc tttgcgccgt ggtctgccca tttggcgcaa tcagcgcttc aggaagccgt
      361 ccggtgaatg cccatgcgca atatgttttt caggctgaag gctcactcaa agacggcgaa
      421 gaaaacgtgc tgccccaaca tgcgttgctg cgctgggaac cgggtgtcca gaccgtcgcg
      481 gtgaaatgcg acctgtgtga tttcttgcca gaaggtccgg cctgcgttcg cgcttgcccg
      541 aatcaggcgt tacggctgat caccgacgat agcctgcaac gtcagatgaa agaaaaacag
      601 cgccttgccg caagctggtt tgccaatggc ggggaggatc ccctttccct cacttaggag
      661 caacactaat ggatgccctg caattattaa cctggtcgct gatcctctat ctgtttgcca
      721 gtctggcttc gctgttttta ctcggtctgg acagactggc tattaagctt tccggcatca
      781 catcgctggt gggcggcgtg attggcatca tcagcggaat tacgcaatta catgctggtg
      841 taactttagt cgcccatttt gccacccctt ttgactttgc cgatttaacc ctgcgaatgg
      901 atagcctctc ggcatttatg gtgctggtta tctccttgct ggtggtggtt tgttcgctct
      961 attcattgac ttatatgcgc gaatacgagg gcaaaggcgc ggcggcgatg ggcttcttta
     1021 tgaatctttt catcgcatcg atggttgccc tgctggtgat ggacaacgct ttttggttca
     1081 tcgtgctgtt tgaaatgatg tcgctgtctt cctggtttct ggtcattgcc aggcaggata
     1141 aaacgtcgat caacgctggc atgctctact tttttatcgc ccacgccgga tcggtgctga
     1201 ttatgatcgc cttcttgctg atggggcgcg aaagcggcag cctcgatttt gccagtttcg
     1261 cacgctttca ctttctccgg ggctggcgtc ggcggtgttt ctgctggcct ttttcggttt
     1321 tggcgcgaaa gccgggatga tgccgttgca cagctggttg ccgcgcgctc accctgccgc
     1381 accatcgcac gcttcggcgt tgatgtctgg cgtaatggtc aaaatcggta ttttcggcat
     1441 cctgaaagta gcgatggatc tgctggcgca aacgggtttg ccgctgtggt ggggcattct
     1501 ggtgatggcg atcggcgcaa tctccgcgct cctgggcgtg ctgtatgcgc tggcggaaca
     1561 ggatatcaaa cggctgctgg cctggagtac cgtcgaaaac gtcggcatta ttttgctggc
     1621 agtcggtgtg gcgatggtcg gtctgtcact gcacgacccg ctgctcaccg tggttggact
     1681 gctcggcgca ctgtttcatc tgctcaacca tgcgctgttc aaatggctgc tatttctcgg
     1741 cgcgggcgcg attatttcgc gtttgcatac ccacgacatg gaaaaaatgg gggcactggc
     1801 gaaacggatg ccgtggacag ccgcagcatg cctgattggt tgcctcgcga tatcagccct
     1861 tcctccgctg aatggtttta tcagcgaatg gtacacctgg cagtcgctgt tctcactaag
     1921 tcgtgtggaa gccgtagcgc tacaacttgc gggtcctatt gctatggtga tgctggcagt
     1981 cactggtggg ctggcagtaa tgtgcttcgt caaaatgtac ggtattactt tctgtggtgc
     2041 gccgcgcagt acacacgctg aagaggcaca ggaagtgcca aatacgatga tcgtcgccat
     2101 gctactgctc gcggcactct gcgtattaat tgcgcttagt accagttggc tggcaccgaa
     2161 gataatgcat attgcccatg cctttaccga tacccctccc gtcactgtcg ccagcggaat
     2221 agcacttgta cccggcacgt ttcatacacg ggtcactccc tcattactgt tgctgttact
     2281 actggcgatg cctttgctgc ctggccttta ctggctgtgg tgtcgttcgc gccgcgcagc
     2341 gtttcgtcgc acaggagatg cctgggcatg cggctacggc tgggaaaatg cgatggcccc
     2401 gtcaggcaat agcgtgatgc agccgctgcg tgtggtcttt tctgcgctat ttcgtctacg
     2461 acaacagctc gaccctacgc tgaggctaaa taaaggtctt gcgcacgtca ccgccagggc
     2521 tcagagcaca gaacccttct gggatgagcg ggtgatccgc cccatcgtga gcgccaccca
     2581 acggctggcc aaagaaatac agcatctgca aagcggcgac tttcgtctct attgcctgta
     2641 tgtggttgcc gcactggttg tgctgctaat cgctattgcc gtctaaggaa atcaccatga
     2701 gacaaactct ttgcgacgga tatctggtca tttttgcgtt agcacaggcc gtgattctgc
     2761 tgatgctaac cccacttttt acgggtattt cccggcagat acgcgcgcga atgcactccc
     2821 gccgcagacc ggggatctgg caggattatc gcgatatcca caaactgttt aaacgccagg
     2881 aagttgcgcc gacatcttca ggtctgatgt tccgcctgat gccgtgggta ttaatcagca
     2941 gcatgctggt gctggcgatg gccctaccac tatttattac cgtttcccct tttgcgggcg
     3001 gcggcgatct gatcaccctt atctgtcttc ttgccctgtt tcgttttttc tttgctcttt
     3061 ccgggctgga taccggaagc ccgtttgcgg gagtcggtgc cagtcgcgag ttgacgctcg
     3121 gcattctggt cgaacccatg ctcattctct cactgctggt attggcgctg atagcaggtt
     3181 ccacgcatat cgagatgatc agcaatacgc tggcaacggg ctggaactcg ccgctaacca
     3241 ccgtactggc gttactggcc tgtggttttg cctgcttcat tgagatggga aaaattccct
     3301 ttgatgttgc tgaagcagaa caggaattac aggaaggccc gctgaccgaa tattccggtg
     3361 ccgggctggc gctagcgaaa tgggggctgg agctgaaaca ggtcgtgatg gcagcactgt
     3421 ttgtggccct gtttctgccc tttgggcgcg cgcaagaact ttctctcgcc tgcctgctga
     3481 cttcacttgt cgttacgctg ctcaaggttt tgctgatttt tgtactggcc tcaatcgcag
     3541 aaaacacgct ggcacgcggg cgttttttac tcattcacca tgtgacctgg cttggcttca
     3601 gccttgctgc gcttgcatgg gtcttctggt taaccggtct gtaaggagca ctgacggaat
     3661 atggaaaatc ttgctctgac gacgttaatg ctgcctttta tcggcgcact ggtcgtttcg
     3721 ttttcgccac aacgtcgggc cgccgaatgg gggttttgtt cgccgcactg accacgctgt
     3781 gcatgttgtc gctgatctcc acgttttatc aggccgataa agttgccgtc acgttgacgt
     3841 tggtcaacgt gggggatgtg gcattgtttg gcctggtcat tgatcgcgtg agtacgctga
     3901 ttctgtttgt ggtggtgttt ctcggtttgc tggtcacgat ctactccacg ggttatctga
     3961 cggataaaaa tcgcgaacac ccgcataacg gcacgaatcg ttattacgca tttttgctgg
     4021 tgtttatcgg cgcgatggcg ggactggtac tctcctcgac gctgctcggt cagttgttgt
     4081 tttttgaaat tacgggcggc tgctcctggg cgttgatcag ttattaccag agcgataaag
     4141 cgcagcgttc ggcactaaaa gcgttgctta tcactcatat cggctcgttg gggttgtatc
     4201 ttgccgccgc cacgctgttt ttgcagaccg gaacgtttgc gcttagcgca atgagcgagt
     4261 tacacggcga cgcacgttat ctggtttatg gcggcatcct gtttgccgcg tgggggaaat
     4321 cggcccagct accgatgcaa gcgtggctac cggatgcaat ggaagcgcca acaccgatca
     4381 gtgcctatct ccacgccgca tcgatggtga aagtgggcgt ttacattttt gcccgcgcca
     4441 ttatcgacgg cggcaatatc ccgcatgtga ttggcggcgt tggcatggtc atggcgctgg
     4501 tcaccattct ttatggattt ctgatgtatt tgccacagca ggatatgaag cggttgctgg
     4561 cctggtcgac catcactcaa cttggctgga tgttcttcgg cttgtcgctc tccatcttcg
     4621 gctcgcggct ggcgctggag ggtagcatcg cctacatcgt caaccacgcg ttcgctaaaa
     4681 gcctgttttt ccttgtagca ggtgcgctga gttacagctg cggcacgcgc ttgttgccgc
     4741 gtctgcgtgg cgtattgcac acactgccgt tgcctggcgt gggtttctgc gtggcagcgc
     4801 tggcaattac cggcgtgccg ccgttcaacg gcttcttcag taaattcccg ctgtttgctg
     4861 ccggttttgc gttgtcagtg gagtactgga tcctgctgcc cgccatgatt ctgctgatga
     4921 ttgaatcggt cgccagtttc gcctggttta ttcgctggtt tggtcgcgtt gtgcctggca
     4981 aaccgagcga ggccgtcgcc gatgccgcac cgctgccagg atcaatgcgc ctggtgttga
     5041 ttgtactgat tgtgatgtcg ctgatttcca gcgtaatcgc cgcgacctgg ttgcagtaag
     5101 gagatgatga atgaccggtt ctacgatcgt aaataatctg gcgggactga tgatgctgac
     5161 atcactgttt gtgattagcg tcaaaagcta tcgcctgtca tgcggatttt acgcctgcca
     5221 gtcactagtg ctggtgtcta ttttcgccac cctctcgtgc ctgttcgccg cagagcaact
     5281 gctgatctgg tccgccagcg cctttatcac caaagtgctg ctggtaccgt taatcatgac
     5341 ttacgctgca cgaaatattc cccagaacat cccggaaaaa gcgttattcg gtccggcaat
     5401 gatggcactg ctcgcggcgt taattgtcct gctttgcgca tttgtcgttc agcccgtgaa
     5461 gctaccgatg gctaccgggc tgaaaccagc gctggcggtg gcgttaggtc attttctgct
     5521 tggcctgctg tgcattgtca gccagcgcaa tatcctgcgg caaatttttg gttactgcct
     5581 gatggaaaac ggctcccatc tggtgctggc gcttcttgcc tggcgagcac cggaactggt
     5641 ggaaataggt atcgctaccg acgccatctt cgccgtcatt gtgatggtgt tactggcaag
     5701 aaaaatatgg cgtacccacg gcacgctgga cgtgaacaac ttgaccgcgc tgaagggata
     5761 atgagatgag ttattctgtg atgttcgctt tactcctgct cacgccgctg cttttttcgc
     5821 tgctctgttt tgcctgccgg aaacgggggc actctgcgac tcgcgcggtg acagtattac
     5881 atagcttagg gatcacactg ctgctgattc tggcactctg gttggttcaa actgccgctg
     5941 gtgcgggaga aatattcgct gcgggactgt ggcttcatat tgatggtctg ggcggtttgt
     6001 tcctcgccat tcttggtgtg attggctttc tcaccggtgt ttactcgatt ggctacatgc
     6061 gtcatgaagt ggcacacggc gagctttcac ccgttacgct gtgcgattac tacggtttct
     6121 tccatctgtt tttgttcacc atgctgctgg ttgttaccag caataacctg attgtgatgt
     6181 gggcggcgat cgaagccacc accttaagct cggcgtttct ggtaggcatt tacggtcagc
     6241 gttcatcgct ggaagctgca tggaagtaca tcattatttg tactgttggc gtcgcttttg
     6301 gtctgttcgg taccgtgctg gtatacgcca acgccgccag cgttatgccg caggcagaaa
     6361 tggcgatatt ctggagcgag gttcttaagc aatagtcctt gcttgaccca acattaatgc
     6421 tattggcctt tgtgtttgtg ctaattggct ttggtaccaa aaccgggcta tttcctatgc
     6481 acgcctggct gccggatgct cacagtgaag cgccgagtcc ggtcagcgcc ctgctctccg
     6541 ccgtattgct gaactgcgcg ctgttggtga tgattcgcta ttacatcatt atctgccagg
     6601 ccatcggcag cgatttcccc aaccggttgt tgctcatctt cggcatgttg tcggttgccg
     6661 tggcggcatt tttcattctg gtacagcggg acattaagcg tctgctggcg tactccagcg
     6721 tggagaacat gggactggtc gcggtggcgc taggcattgg cgggccgctg ggaatttttg
     6781 ccgcgctgct gcacacctta aaccacagtc tggcaaaaac gctgctgttc tgcggttccg
     6841 gcaatgtact gctcaagtac ggcacgcgcg atctcaacgt cgtctgcggg atgctcaaaa
     6901 tcatgccatt taccgccgtg ctgtttggcg gcggtgcgct ggcgctggcc gggatgccgc
     6961 ccttcaacat ttttcttagc gaatttatga ccgttaccgc cggactggca cgtaatcacc
     7021 tgctgattat cgtcatgctg ttattgctgt taacgctggt gctggcgggc ctggtacgga
     7081 tggctgcgcg ggtgttaatg gcgaaaccgc cgcaggccgt taaccggggt gatctcggct
     7141 ggttgaccac ctcgccaatg gtgattctgc tggtcatgat gctggcgatg ggaacgcata
     7201 ttccacaacc tgtcatcagg atcctggcgg gcgcttccac tatagtcctc tcagggacgc
     7261 acgacctgcc tgcacaacgt agcacctggc atgatttttt gccttcaggc accgcatctg
     7321 tttcggagaa acacagtgaa cgttaattca tcgtcaaatc gtggcgaagc gattctcgcc
     7381 gccctgaaaa cgcagttccc cggcgcggtg ctggatgaag agcgacaaac gcctgaacag
     7441 gtcaccatta cggtgaaaat caatctgctg cctgacattg tacattatct ttattatcaa
     7501 catgatggct ggcttccggt tctgtttggc aacgacgagc ggacacttaa cggtcattac
     7561 gcggtttatt atgccctttc tatggaaggg gccgaaaaat gctggatcgt agtgaaggca
     7621 ctggtcgatg ccgacagtcg ggagtttccg tcagtcacac cgcgcgtccc tgccgcggtc
     7681 tggggcgagc gagaaattcg cgatatgtac gggctgattc cggttggcct gccggatcag
     7741 cgtcgcctgg tgttgcccga tgactggccg gaagatatgc atccgctgcg caaagatgcg
     7801 atggattatc gactgcgccc tgaaccgacg actgattccg aaacgtatcc gtttatcaat
     7861 gagggcaaca gcgatgcgca ggtgatccct gtcggcccgc tgcatatcac ctccgatgaa
     7921 ccgggtcact tccgcttgtt tgtggatggc gagcaaattg tcgatgctga ttaccgcctg
     7981 ttttatgtcc atcgcggcat ggagaaactg gcagaaacgc ggatgggcta caacgaagtg
     8041 accttcttat cagaccgcgt gtgtgggatt tgcggttttg cccacagtgt ggcctatacc
     8101 aactcggttg aaaatgcact ggggattgag gtgccgcaac gagcgcatac cattcgctcg
     8161 attctgctgg aagtcgaacg gctacacagt catttgctca accttggcct ctcctgccat
     8221 tttgttggtt ttgataccgg ctttatgcaa tttttccgcg tgcgggaaaa gtcgatgacg
     8281 atggcggaat tgctgaccgg gtcgcgtaaa acctacggtc tgaatctgat tggtggtgtt
     8341 cgccgcgata ttctcaaaga gcaacgtctg caaacgctga aactggtgcg cgagatgcgc
     8401 gccgacgtgt cggagctggt agagatgctg cttgctacgc cgaatatgga acaacgcact
     8461 cagggcattg gcattctcga ccgacaaatc gcccgtgatt atagccctgt agggccgctg
     8521 atccgcggca gtggttttgc ccgtgatttg cgctttgatc acccctacgc cgactacggc
     8581 aatattccaa aaacactgtt taccttcacc ggcggcgatg ttttctcccg cgtgatggtc
     8641 cgtgtcaaag agacgtttga ttcgctggca atgctggaat ttgccctcga caacatgccg
     8701 gataccccgc tgctgaccga aggctttagc tataaacctc acgcattcgc gctgggcttt
     8761 gttgaagcgc cacgcggtga agacgtgcac tggagcatgc tcggtgataa ccaaaaattg
     8821 ttccgctggc gctgtcgtgc cgccacctac gccaactggc cagtgctgcg ttacatgcta
     8881 cgcggcaata ccatttctga cgcaccgctg attatcggta gccttgatcc ctgctactcc
     8941 tgtaccgacc gtgtgacgct ggtagatgta cgcaagcgcc agtcaaaaac cgtgccgtat
     9001 aaagagatcg aacgctacgg cattgatcgt aaccgttcgc cgctgaagta aggacagaag
     9061 atgctgaagt tactgaaaac tattatgcgc gccggaaccg cgacggtgaa atatcccttc
     9121 gcgccactgg aggtcagccc aggctttcgc gggaaaccgg acctgatgcc cagccaatgt
     9181 attgcctgcg gtgcctgcgc ctgtgcttgt ccggcaaatg cgctgactat ccagaccgac
     9241 gaccagcaaa attcgcgcac ctggcagctc tatctggggc gttgtattta ctgcggacgt
     9301 tgtgaagaag tgtgcccgac cagagccatc cagcttacca ataactttga actgaccgtc
     9361 accaataaag ccgatctcta tacccgcgcg acgttccatc tacaacgttg cagccgttgc
     9421 gaacgcccgt ttgccccgca aaaaaccgtc gcactggctg ctgaattgtt ggcacagcaa
     9481 caaaatgcgc cacaaaaccg cgaaatgttg cgggcgcaag cgagcgtctg cccggagtgc
     9541 aaacaacgcg cgacgctgat caacgacgat acagatgtac cgctggtggc taaggagcag
     9601 ctatgagtcc agtgcttaca caacatgtca gccagcccat cacgctggat gagcaaacgc
     9661 aaaagatgaa gcggcatttg ctacaggata tccgtcgctc ggcttacgtt tatcgcgtcg
     9721 attgcggcgg ctgcaacgcc tgtgaaatcg aaatttttgc cgccatcaca cccgtattcg
     9781 acgcagaacg ttttggcatt aaggttgttt catcaccgcg tcacgccgat attttgttat
     9841 ttactggcgc agtcacccgg gcgatgcgta tgcctgcact tcgggcgtat gagtctgccc
     9901 ccgatcataa aatttgtgtt tcctacggcg cgtgcggtgt cggcggcggt attttccacg
     9961 atctctacag cgtctggggc ggtagcgaca ccattgtccc cattgatgtt tggatccccg
    10021 gctgcccgcc aacaccggcc gccaccattc acggtttcgc cgtggcgctc ggtttgctgc
    10081 aacagaagat tcacgctgtg gattatcgcg atcccaccgg agtgaccatg caaccgctgt
    10141 ggccgcagat cccgccatca cagcgtatcg ccattgagcg agaagcgcgg cggctggcgg
    10201 gctatcgtca ggggcgagaa atttgcgatc ggctcttgcg ccatttaagc gatgatccta
    10261 caggaaatcg ggttaacacc tggttgcgcg aagccgacga tccgcgtctc aatagtatcg
    10321 ttcagcaact ctttcgcgtt ctccggggat tacatgactg aagagtgcgg ggaaattgtt
    10381 ttctggacgc tgcgaaaaaa gtttgtcgcc agtagcgacg agatgccgga acacagctcc
    10441 caggtaatgt attactcgct ggctatcggt catcacgttg gcgtgattga ttgtctgaat
    10501 gtcgccttcc gctgcccact gacggaatac gaagattggc ttgcccaggt cgaagaggag
    10561 caagcccgac gtaagatgct gggggtgatg acttttggtg agattgttat tgacgccagc
    10621 cacaccgccc tgttgacccg ggcatttgcg ccactggcgg ataacgcgac gtctgtgtgg
    10681 caggcgcgta gcattcaatt cattcatcta ttggatgaaa ttgtgcagga accggccatc
    10741 tatctgatgg ccagaaaaat tgcgtgagaa ggatttctca ttaataagga ctgttgatgg
    10801 ctatgtcaga cgaggcgatg tttgccccgc cgcaaggaat aacaattgaa gcggtaaacg
    10861 gaatgctcgc ggagcggtta gcacagaaac acggtaaggc gtctttatta cgcgccttca
    10921 tcccgctgcc gccgccgttc agcccggtac aacttattga actgcatgtt ctcaaaagca
    10981 acttctatta ccgctaccat gatgatggca gcgatgtgac ggcaacaaca gagtatcagg
    11041 gcgagatggt cgattattcg cgtcacgccg tccttctcgg cagtagtgga atggcggagc
    11101 tacgctttat tcgtacccac ggcagtcgtt ttacttccca ggattgcaca ctgtttaact
    11161 ggctggcgcg gataatcacc ccggttctgc aatcatggct caatgatgaa gaacagcagg
    11221 tggcgcagcg tttgctggag aaagatcgcg atcatcatcg ggtactggtt gatattacta
    11281 atgcagtgct gtcacatctt gatctcgacg atctgatcgc tgacgtcgcc cgtgagatcc
    11341 atcatttttt cggtctggct tcagtcagta tggtactggg cgatcatcga aagaacgaga
    11401 agttcagcct gtggtgcagc gatctttctg cctcacattg tgcgtgtctg ccacgcaata
    11461 tgcctggcga cagtgtattg ctgacacaaa cgctacaaac ccgacaaccg accttgacgc
    11521 atcgtgcaga tgatctgttt ctctggcaac gcgacccgtt attactctta cttgcatcta
    11581 acggctgcga atctgcgctc ctgataccgc ttacctttgg caaccataca ccgggtgcat
    11641 tgttgctggc gcatacctct tccactctct ttagtgagga aaactgccag ctactacaac
    11701 acatagccga tcgcatcgct attgccgttg gcaatgccga tgcctggcgc agcatgaccg
    11761 atttgcagga aagtttgcag caagaaaacc accagcttag cgagcagctc ctttcaaatc
    11821 tgggcgtcgg tgacattatc tatcaaagcc aggcaatgga agacctgctc cagcaggtag
    11881 atattgtggc gaagagcgac agtacggtgt tgatttgtgg tgaaaccgga accggcaaag
    11941 aggtgatcgc cagagcgatc catcaactta gcccgcgaca cgacaagccg ctggtcaaaa
    12001 tcaactgcgc tgccatcccc gccagtcttc tggaaagtga gttattcggt catgacaaag
    12061 gggcgtttac tggtgcgatt aatacccatc gtggtcgttt tgaaattgcc gatggcggca
    12121 cgttgtttct cgatgaaatt ggcgatctgc cgttagaact tcagcctaaa ctgctgcgcg
    12181 tattgcagga gcgggagatt gagtgtctcg gcgggagtag aacgatcccg gtgaatgtca
    12241 gagtcattgc cgccaccaac cgtgatttgt ggcaaatggt tgaagatcgc cagtttcgca
    12301 gcgatctctt ttatcgcctg aatgtcttcc cactggaatt gccgccgctg cgcgaccgtc
    12361 cggaagatat ccctctttta gcaaaacatt tcacgcaaaa aatggcgcgc catatgaatc
    12421 gcgcaattga cgccatccca accgaggcgc tacgccagtt gatgtcgtgg gactggccgg
    12481 gcaacgtgcg cgagctggaa aacgtgattg agcgagcggt actgctgact cgcggtaaca
    12541 gtctgaattt acatcttaat gtccgacaaa gccgtttact gccgacgcta aatgaagatt
    12601 cagcgcttcg cagttcaatg gcgcagttgc tgcacccgac gacgccagag aatgacgaag
    12661 aagaacgtca gcgcattgtt caggtattgc gagaaaccaa tggcattgtt gccgggcccc
    12721 gtggcgcggc gacacgatta gggatgaagc gcaccacgct gctgtcacga atgcagcgtc
    12781 tggggatctc ggttcgcgag gtgttgtaat ctgcttttgc aggagtatgc atgagaaaca
    12841 aactctcttt cgacttgcag ttgagcgcca gaaaagcggc aatcgctgaa cggattgctg
    12901 cccataaaat tgcccgcagc aaagtatcgg tctttttaat ggcgatgtcc gctggcgtgt
    12961 ttatgacgat tggatttact ttttaccttt ccgttatcgc cgatgccccg tcttcacagg
    13021 cattaaccca tctggtgggc ggcctttgct ttacactcgg ctttattttg ctggcggttt
    13081 gcggcaccag cctgttcacc tcgtcggtaa tgacggtgat ggcaaaaagt cggggcgtta
    13141 ttagttggcg aacttggctg attaacgcac ttctggtggc ctgcggtaat ctggcaggta
    13201 ttgcctgttt cagtttgtta atctggtttt ccgggctggt gatgagtgaa aacgcgatgt
    13261 ggggagtcgc ggttttacac tgcgccgagg gcaaaatgca tcatacattt actgaatctg
    13321 tcagcctcgg cattatgtgc aatctgatgg tttgcctggc gctgtggatg agttattgcg
    13381 ggcgttcgtt atgcgacaaa atcgtcgcca tgattttgcc catcaccctg tttgtcgcca
    13441 gtggctttga gcactgtatc gccaatttgt ttgtgattcc gttcgccatt gccattcgcc
    13501 atttcgcccc tccccccctt ctggcaactg gcgcacagta gcgcagacaa ttttccggct
    13561 ctgacggtca gccattttat taccgccaat ctgctcccgg tgatgctggg taatattatc
    13621 ggcggtgcag tgctggtgag tatttgttat cgggctattt atttacgtca ggaatcctga
    13681 aaaatcagcc cggcgaaaca gttcgtcggg ctgaagatta ttcttgcgct atttgcccat
    13741 cgggccaggc gtggatcaca gctttgatca gggtcgccaa tggaatggcg aagaatacgc
    13801 cccagaatcc ccacaaacca ccgaagatca ccaccgataa aataatcacc agcggatgca
    13861 ggttaaccgc ttcggagaac aacaccggta ctaacaggtt gccgtccagc gcctgaataa
    13921 tcagatacac tgcgaagcag ctccagaatt ccgtgcctgc accaaactgg aatagcgcca
    13981 cgccaaccac cggaatggtc accacaaatg cgccaatgta cggaataagt accgagaagc
    14041 cgaccagcac cgccagcagc agcgaatagt tcagcccaaa gagcaagaac cccagccagg
    14101 tggcgatccc caccacgatc atctccagca ctttgccgcg gatatagttg gtgatttgtt
    14161 gattcatctc cttccacacc tgtcctgcca gtccacggtt gcgcggcagc acccgacgaa
    14221 cggcgttcag catctgctct ttgtctttca gcaggaagaa gaccatcaat ggcaccagca
    14281 ccagatagac ggctatggtc agcaaaccga ccagcgaggc gagggaaatt ttcaccaccg
    14341 aatcgcccat ggtcagcatc cgactgcgca tattttcggc cattgcatca ataatgcccg
    14401 catccattaa cgccggatag cggcgcggca acgtggcggc aaagtcagaa agcttattga
    14461 gcatccccgg catatcgcgg attaagtaga tgccctgttg ccaggcgata ggcagtacca
    14521 cgaacgccat cagtagcaat ataccgacga aaaccaccaa tacaatcgac gtcgcccagc
    14581 ggcgggagca gccaatagat tgcaggcgca cggttggcca ttccagcaaa taggccagca
    14641 caatagccac cagcaacgga gcaagcaggc cactaaagaa aaagataatg ccaaatccgg
    14701 caactaaaat aaccagcaag gcaatcgctt ccgggtcgct aaaacggcgg cgataccatt
    14761 gcatcaacat ttcgagcata aagaccttcc ctgaacctca agagcgggat tgcgatcagc
    14821 aattgtatcg aaatgtcaca aaaaagactt cgctttttat gacggattca ggaaactgaa
    14881 aagtcatttg agtgggctaa tcttcgccgt tacactcaaa ggcggcgcgg tgggaacgat
    14941 atttcacagt atcggtcaaa tgactatctt cagaaataca ggatagaggt tatgttcagg
    15001 cagttgaaaa aaaacctggt tgcaaccctc attgctgcta tgaccattgg tcaggtagcc
    15061 ccggcgtttg ccgacagcgc agacaccttg ccggatatgg gaacctccgc aggaagcacg
    15121 ctttccattg gtcaggaaat gcagatgggc gactattatg tccgccagct acgcggcagc
    15181 gcgccgttaa ttaatgaccc gctgttaacg caatatatta attcgctggg gatgcgtctg
    15241 gtttcgcatg ccaattcggt taagacaccg tttcattttt ttctgatcaa caacgacgaa
    15301 attaacgcct ttgctttctt tggcggcaac gtggtgctgc actctgccct gttccgttat
    15361 tccgataacg aaagtcaact ggcttcagtt atggcgcacg aaatctccca cgtcacccaa
    15421 cgtcacctgg cgcgagcgat ggaagatcag cagcgcagcg cgccgctgac ctgggtcggc
    15481 gcgttaggtt ctattttact ggcgatggcc agtccgcagg cggggatggc ggcactgacc
    15541 ggtacactgg cgggaacgcg ccagggaatg atcagcttca cccagcaaaa tgaacaggaa
    15601 gcggaccgca ttggtattca ggtgctgcaa cgctcgggat tcgatccgca ggcgatgcca
    15661 accttcctcg aaaaattact cgatcaggcg cgttactcct cgcgcccgcc ggaaatttta
    15721 ttgactcacc cgttgccgga aagtcgtctg gcagatgccc gcaaccgtgc taatcagatg
    15781 cgcccgatgg tggtgcagtc gtcggaagat ttctatctgg cgaaagcgcg cacactgggg
    15841 atgtataatt ccggacgtaa ccagctcacc agtgatttgc tggatgaatg ggcgaaagga
    15901 aacgttcgtc agcaacgagc agcacaatat ggtcgtgctt tacaggcgat ggaagccaat
    15961 aaatacgacg aggcgcgtaa aacgctgcaa ccgttactgg cggcagaacc tggcaacgca
    16021 tggtatctcg atctggctac cgatatcgat cttgggcaaa acaaagccaa tgaggcgatc
    16081 aatcgtctga aaaatgcccg cgatttacgc accaatcctg tgttgcagct caacctggcg
    16141 aacgcttatc tacaaggcgg tcaaccacaa gaagcggcca atattctgaa tcgctacacc
    16201 tttaataata aagatgacag caacggctgg gatttgctgg cacaggcgga agccgcgcta
    16261 aataaccgcg atcaggagct ggctgcgcga gcagaaggtt atgcgctcgc cgggcgactc
    16321 gatcaggcca tttcgctgtt gagtagcgcc agttcgcagg tgaaattagg cagcctgcaa
    16381 caagcgcgtt acgatgcgcg catcgaccag ttgcgccagc tgcaggaacg ctttaagcct
    16441 tataccaaga tgtaattcgc attaaaggaa gaacaatgac caaacaggta aaaatctacc
    16501 ataacccacg ctgttcaaag agccgggaaa cgctgaacct gctgaaagaa aacggcgtgg
    16561 agccagaagt ggtgctctac cttgagacac ccgccgatgc tgcaacgctg cgcgatttgc
    16621 tgaaaatgct ggggatgaat agcgcccgtg aattgatgcg ccagaaagag gatctttata
    16681 aagaactgaa cctggctgac agttcgctta gcgaagaggc gctgattcag gcaatggtgg
    16741 ataatccgaa gctgatggag cgcccgattg tggtcgcgaa tggcaaagcg cggattggtc
    16801 gtccaccgga gcaggtactg gaaatcgtgg gttaagatgt gctccatcgc tagttgaagc
    16861 acattgccgg atgcgacgct tgccacgtct tatccggcct acgaatacta tggattaact
    16921 tcgcgtagtt cggataaggc gttcgcgccg catccgacaa taaacacctt atctacaact
    16981 tcagaatttc tttcacaaac ggaatggtca gcttacgttg cgcggtaatc gacgcacgat
    17041 ccaactgatc caacgtcata aatagcgtgc gcatttctct gtcgagccgc ttcagcaaga
    17101 aacgccccac atcttccggc agttcaaaac cacgcaaacg cgcgcgtaac tgtagcgcct
    17161 gcaacttatc ttcatcagaa agtggctgca atttgtagat ctgcccccag tcgagtcgcg
    17221 acgcgagatc cggtaatccc agattcaact gccgcggtgg acgatcgccg gtgatcaaca
    17281 accgtgtttt gcccgattcc agaattcgat tgtagagatc gaaaatcgcc atctcccaca
    17341 actcatcgcc tgcaatacac tcaatgttgt cgatacagac cagcgacaaa tgctccatac
    17401 cgtcgagcac ttccggaaca aaccaggtgc gtttatccag cgggacatag cccaccgcat
    17461 cgccacgctg cgacaattcc gcgcaagccg cgtgcagcag atgactgcgc cccgcgcctt
    17521 cgcgtgccca gagatagatg taaccgctat gttcctgacg cagcacgttt tgcagcgcgg
    17581 ccagtaaaga ggagttatcc cccggccaga aacttgcaaa ggtttcgtcg tcaggaagat
    17641 aaagtggcaa agagagctgt gccggtgtgt tcagagatac ctcaaccagg atttcacaaa
    17701 atcgcgagaa gtttaccaca gaatcccatg atgtttgaac cgggcagcaa cactgcccgg
    17761 tcggtacatt atttgtctgt tatatccgcg tcttctgcgt ccagcaccac ttcttcaggg
    17821 cgcagcacac tgatcagttt gaaaatcaaa cttaagccga taccgacgat ggtcgccagc
    17881 gccatacctt tcaactccgc cgcgccgatg tttaccttcg cgccactgac gccgatgatc
    17941 aaaatcacgg aagtcaggat caggttctgt gctttgttgt aatccacttt cgattcgatc
    18001 aaaacacgaa taccggaagc accgatgaca ccataaagca gcagcgaaac gccgcccatc
    18061 accggcaatg ggatcatctg gatagcggca gccagtttac cgacgcagga aagcaggata
    18121 gcgaaaatcg ccgccccgcc gataacccag gtactgtaaa cacgggtgat cgccatcacg
    18181 ccaatgtttt ctccgtaagt ggtatttggc gtagagccaa agaagccgga aatcacggtc
    18241 gacaagccat tagcaaacat cgaacggtgc agacctggat cgcgcagcag atcttttttg
    18301 acgatattag ccgttactac caggtgccct acgtgttcgg caataaccac taacgccgct
    18361 ggcagaatag tcagaatggc aaaccactcg aagcgcggcg tatagagggt tggcagcgca
    18421 aaccagtgag cattaataat cggcgtggta tcgacaattc ccattgcgaa agagagcgcg
    18481 taccccacca gcacgccaat taaaatcggg ataattgcca ggaaaccacg gaacagcacg
    18541 gaacctaaaa ccgtgaccgc cagggtggta atagagatga tgatggtttt ggagtctggc
    18601 gtttgccctt cagccgggag taaacccgcc ataccggcag ctacgcccgc cagctccaga
    18661 ccgatgacgg caacgattgc gcccattgcc gcaggtggaa acagcacgtc cagccagccg
    18721 gtccccgctt tcttcacgat aaaagaaacc aggcagaaca gcacgccgca cataataaag
    18781 ccgcccagcg cgacttcata ccctaacggc aacagtaaca ataccggtga aataaaggca
    18841 aagctggaac caagataagc cggaattttc cctttacaga tgaagagata cagcagcgtt
    18901 ccaataccgt taaataacag tacagtcgcc gggttaatat gaaataagac gggcaccagg
    18961 acggttgcac caaacatggc gaacaaatgt tgcaaactaa gcgggattgt ct
//
LOCUS       DAPHBJ010000082        18870 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.082, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000082 DAPHBJ010000000
VERSION     DAPHBJ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.082"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            33..902
                     /locus_tag="RHZ76_002493"
     CDS             33..902
                     /locus_tag="RHZ76_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HDV2813113.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1095..1448)
                     /locus_tag="RHZ76_002494"
     CDS             complement(1095..1448)
                     /locus_tag="RHZ76_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HDV2813114.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1586..3232)
                     /gene="groL"
                     /locus_tag="RHZ76_002495"
     CDS             complement(1586..3232)
                     /gene="groL"
                     /locus_tag="RHZ76_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDV2813115.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3276..3569)
                     /locus_tag="RHZ76_002496"
     CDS             complement(3276..3569)
                     /locus_tag="RHZ76_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDV2813116.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3845..5101
                     /gene="yjeH"
                     /locus_tag="RHZ76_002497"
     CDS             3845..5101
                     /gene="yjeH"
                     /locus_tag="RHZ76_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HDV2813117.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            complement(5117..5593)
                     /gene="fxsA"
                     /locus_tag="RHZ76_002498"
     CDS             complement(5117..5593)
                     /gene="fxsA"
                     /locus_tag="RHZ76_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HDV2813118.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            5930..7366
                     /gene="aspA"
                     /locus_tag="RHZ76_002499"
     CDS             5930..7366
                     /gene="aspA"
                     /locus_tag="RHZ76_002499"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDV2813119.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7484..8785
                     /gene="dcuA"
                     /locus_tag="RHZ76_002500"
     CDS             7484..8785
                     /gene="dcuA"
                     /locus_tag="RHZ76_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HDV2813120.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8901..9239
                     /gene="cutA"
                     /locus_tag="RHZ76_002501"
     CDS             8901..9239
                     /gene="cutA"
                     /locus_tag="RHZ76_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HDV2813121.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9215..10912
                     /gene="dsbD"
                     /locus_tag="RHZ76_002502"
     CDS             9215..10912
                     /gene="dsbD"
                     /locus_tag="RHZ76_002502"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HDV2813122.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRIVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10949..11524
                     /locus_tag="RHZ76_002503"
     CDS             10949..11524
                     /locus_tag="RHZ76_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV2813123.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11631..11706
                     /locus_tag="RHZ76_002504"
     tRNA            11631..11706
                     /locus_tag="RHZ76_002504"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11664..11666,aa:Phe,seq:gaa)
     gene            complement(12792..13445)
                     /locus_tag="RHZ76_002505"
     CDS             complement(12792..13445)
                     /locus_tag="RHZ76_002505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813124.1"
                     /translation="MARTEEERAQATSIFATYKAEVDKRELSNTDNYDKNILTLSSAG
                     LAISLTVFKDIVPHNQTAHIWLLYSAWVLFGCAILSTIFSFLISNAALRAQLEIAHKY
                     YIEEDESVFGKVSLESKALDWVNRFSGGFFVLAIISVITFGVINFDRRTEVSKSDMVN
                     KTQQINNDGLPPPMMQRVPVDKGATVPLMQPVTQTNQTDATVNGTPSPTTSTDSAKN"
     gene            complement(13468..13716)
                     /locus_tag="RHZ76_002506"
     CDS             complement(13468..13716)
                     /locus_tag="RHZ76_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813125.1"
                     /translation="MAINKKQSSPKLASQAAKALQDENSSEIKKKLAASVVAQARTGK
                     QTGAEMETVASDVMKSSKYSEETKSYAASLLSQSNKPR"
     gene            14437..15621
                     /locus_tag="RHZ76_002507"
     CDS             14437..15621
                     /locus_tag="RHZ76_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017382944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV2813126.1"
                     /translation="MSTPKRFRFTNQLIKSLPPNTSDARSTDAEYSDTEISGLKCLVS
                     RREGRKKFLLRYLYHGRKRAIAIGHWPEVDVNLARQIAIEHKRSLATGTDPKQERENK
                     RMELTFDELFNQHYLPWAKSAKRSWGKDVQRYRDHIRPAIGQRLLSDITPASILRLQQ
                     TLSTSLSAATCNRVIVLIKAVFTWAGRQSMTSVHPARVVQLLRENNARQRYFTEDEIR
                     RIFLSADNDTSPVAARYVKLLLLTGLRRDELRLAKWEHVDPVKRTLWLPETKNGYGRI
                     VHLNSLAMDVIRTLPTQRGNPWLFIGKKPGMPLSNPVKAFQRIVRRAGIFDKEVCIHT
                     CRHSVAALIVSYGGTLYDVQAQLGHRSSQSSQRYAHLHPQRLQNTSQLLAERITAQLP
                     SL"
     gene            complement(15676..16032)
                     /locus_tag="RHZ76_002508"
     CDS             complement(15676..16032)
                     /locus_tag="RHZ76_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017382945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HDV2813127.1"
                     /translation="MIFESDQYYDGFPHAISSENEEKTSFNDYALVNTLAECLYHDIS
                     REIFDGSEPALRAVIANGQIKFLARYTLRGACYYEPLGRFPRMSIKDARECARELYNR
                     YDDNRVPDEEYGAIPF"
     gene            complement(16082..17995)
                     /locus_tag="RHZ76_002509"
     CDS             complement(16082..17995)
                     /locus_tag="RHZ76_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017382946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HDV2813128.1"
                     /translation="MNKKVTINVPKVSGIKRIRAFADRDKNFNKMSEAIIRRAENTEV
                     GEDDIASLGIGDGLPRHYVNTADGLFYIGGQKPVQVCTTPVVPVALFCGKDKESGLGI
                     SLMTGNGLGKYVKAQVSFEDIVQGGAQVMGRLAGRGFCVGPSPQQLALFDRFIQSCRS
                     LPLPVYLMADAMGFVSEDMAFLHGHEPVVTSLTGFDYLLPGFRVRPGLHKGGALEEWI
                     NLIRENVHGWAQTFALSASFASMLLVPAGMDVGMFHFHGVSTTGKTLLLMLAASVHGD
                     GSEPGSGGNVNIIRWNTTPNAMERLLADYSGLVACVDELGASNHKQLSSLLYNITSGT
                     AKERLNRGLTADEPFKWRMFILSTGEMSISEKLAEQKAVLQGGQEHRAISMELLPEDA
                     QKPDEPVSETRARADALKTGLAEAYGHAGPAFIGKLLSGKNTAGGLRTYAEFSSWLQE
                     QTDAYCNALEEALRDEGRDITRVQRRGLKRFALVWLAGEKALKYGLLPFESDAVAESV
                     MSAARRWLDDNQNQYNPVRNMLEELQLTLVTKSSRHFITLRDEHARCPGDMWGFVHYK
                     TGDFLIYESVFEKLCSEKGLKTVDVARPLEHSGYLLGESAGHYKKRALKTMAQSYYQV
                     KKAFLKCNVMTEF"
     gene            complement(18081..>18870)
                     /locus_tag="RHZ76_002510"
     CDS             complement(18081..>18870)
                     /locus_tag="RHZ76_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017382947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV2813129.1"
                     /translation="RSARTDLSKIHKYLLPRLGDKKIADMTEADIVAYLHDLDLKPAT
                     RNRHLALIKAVFTWAIRMGYISRSPALYIKALPEVTSDRRAMDDSQLQCWMDVCERWR
                     NGNPDHEALALLMFLALTGLRLGETRHLRLEDVDWSRKVITLRHTKNGRLRRVPVCDK
                     AFVLLTEQRQHLGDEGWVFPGKGTDNPVAEPRRLQKRLCEAAGIPPFTIHEMRHTFAT
                     KLIESGADIHTVKDLLGHSTIKVTELYLHASPARYHEVVNRAMG"
BASE COUNT         4624 a         4877 c         4512 g         4857 t
ORIGIN      
        1 atttaattga gaaatggtta gggagaacct acatggccat aagcatcaag ggtgttaata
       61 ccggtgttat tcgcaagagt aataatttca ttgcactggc gctgaaaatt aaagaacctc
      121 gcaataaaga atcgttgttc tttatgtctg tcatggaact gcgcgacctg ctgattgcgc
      181 tggaaagtcg tctgcatcaa aagcataaac tggatgctgc tgcacgcctt cagtatgagc
      241 aagctcgcga taaagtcatt aagaaaatgg cagaaaacat cccagaaatt ctggttgatg
      301 agcttaaaaa tgccgatatt aatcgtcggg ttaacacgct ggaacttacc gataatcaag
      361 gcgaaaatct taccttcgta ttaacgctac atgacggtag caaatgtgag ttagtcgtca
      421 atgagttgca gattgaaatg ctggcgcgag caatcattca tgcaattaat aatgcggaaa
      481 tgcgtgaact ggcattacgt attacctcac tgttagattt cctgccgtta tatgacgttg
      541 actgccagga aaatggcaat ctggaatatg acacctattc ccaaccggaa tggaaacata
      601 acttgttcga ccattatctg gcggtgcttt accgctttaa agatgaaagt ggcaaagaac
      661 aatttagcgg cgcagtggtg aaaacgcgtg aagcaacgcc gggtaaagaa atagaggcga
      721 tcactcgccg aatgcttgat ttcagcccac gactgaaaaa actcgcgggt gtgccatgtc
      781 aggtctatgt tcgcaccgta gcggcgaata acgcccaacc gctaacccag gatcaatgct
      841 tacgcgcgct gcatcactta cgtgttcaat ccaccagcaa aactgcacca caggcgaaat
      901 aatctcatct ttgctaatgc gaggctggag ccatccagcc tgaaatgact gataacgtga
      961 tcgttggtca tgccggatgc ggcgtaaacg ccttatccgg cctacaaatt ctcaagcatt
     1021 caatcagttg caatgcctta tgtaggcctg ataagcgaag cgcctctggc aattatacgt
     1081 ttgtcaccat cacatcagtt cgggcactta taaacctgac cgataatctg gctatccgtc
     1141 gggacaaaac tggacaacat tccctgcgac gggctgctga tgccataaat cacgttaccg
     1201 cccattgcag ccgcctggtt gcgcagatcg tttgctgcgc cgcgcataga accgccctct
     1261 tctccgtgtt gcccggaaag ccagttgctt tgcttacctg tcgcagtacc aatcagctgg
     1321 cactctgcgc ctggctgctc gtccacaatg cgtacactct gaccggcagc actcaattcg
     1381 ttgctggagc tacaacccgc catcagtagc gcggcaccga caatccctgc taagtatttt
     1441 acgtgcatgt tattccccat aatcaatgag ctggacgcca ctcgcgtcgt ccgtgtctga
     1501 atcttatact agaaagatga ccaaaagaaa aacccccaga catttctgcc cgggggtttg
     1561 tttatttctg cgaggtgcag ggcaattaca tcatgccgcc catgccaccc atgccgccca
     1621 taccgccagc agcgcctaag tcagctgcat catttttcgg caggtcggta accatgcact
     1681 cggtggtgat catcaagcca gccacagaag ccgcgtactg cagagcagaa cgggttactt
     1741 tggttgggtc caggataccc atgtcgatca tgttgccgta ttcttcggtt gctgcgttgt
     1801 aaccgtagtt gccgtcgccg cctttaacgg tgttagcaac aacagacggt tcttcgccgc
     1861 agttcaggac gatctgacgc agcggagctt ccattgcacg cagtgcaact ttgataccca
     1921 cgttctggtc ttcgttctga ccacgcaggt cagccagttt agacgctacg cggatcagcg
     1981 caacaccacc accagcaacc acgccttctt ctaccgcagc acgggtcgcg tgcagggcgt
     2041 cttcaacgcg tgcttttttc tctttcattt caacttcggt agcagcacct actttgataa
     2101 ctgcaacgcc gcctgccagt ttcgctacgc gttcctgcag tttttcacgg tcgtagtcag
     2161 aagttgcttc ttcaatctgc tgacggatct gagcaacacg gccctggatt gcagcttctt
     2221 cacccacgcc atcgatgata gtggtggtgt ctttgttgat cacaacacgt ttagcctgac
     2281 ccaggtcttc cagggttgct ttttccagct ccataccgat ctcttcagag attacggtac
     2341 cgccagtcag ggttgcgata tcctgcagca tagctttacg acgatcgccg aagcccggag
     2401 ctttaaccgc agcaactttc acgatgccac gcatggtgtt aacaaccaga gttgccagcg
     2461 cttcgccttc aacatcttca gcgatgatca gcagcggttt gcctgctttg gcaacagctt
     2521 ccagaaccgg cagcatttcg cggatgttag agattttctt gtcagccagc aggatgaacg
     2581 ggctttccag ttctactgcg ccagtttccg gcttgttgat gaagtaagga gacaggtagc
     2641 cacggtcgaa ctgcatacct tcaaccacgt ccagttcgtc ctgcagaccg gtaccgtctt
     2701 caacggtgat aacgccttct ttaccgactt tgtccatcgc ttcagcgatc agtttaccta
     2761 cggtttcgtc ggagttagcg gagatggtac caacctgagc aatcgcttta gagtcagagc
     2821 acggtacgga cagcgctttc agttcttcaa ctgcagcggt aacggctttg tcgataccac
     2881 gtttcaggtc catcgggttc atgcccgcag caacagcttt cagaccctca gtaatgatag
     2941 cctgagccag tacagttgca gtggtggtac cgtcgcctgc agcgtcgttc gctttagagg
     3001 caacttcttt caccatctgc gcacccatgt tttcgaactt gtcttccagt tcgatttcac
     3061 gagcaacgga aacaccatct ttggtgatgg tcggtgcacc gaaagattta tccagaacta
     3121 cgttacggcc tttcgggccg agggtaactt tcactgcatc tgccagtacg tttacgccgc
     3181 gcagcatttt cacacgagcg tcgttaccga attttacgtc tttagctgcc attatcttta
     3241 ttccttaaat tcgtatgttc agtgtcgtgc gaggattacg cttcaacaat tgccagaatg
     3301 tcgctttcgg acatgatcaa cacttcttca ttgtcgatct tctcagattt cacaccgtag
     3361 ccatcgttga aaataacgat gtcgccaact ttcacatcca gcggcttcac ttcgccattt
     3421 tcaaggatac ggccattgcc gacagccagc acttcgccgc gggtggattt agccgctgca
     3481 gagccggtca gaacgatgcc gccagcagat ttagtttcaa cttctttacg cttgacgatc
     3541 acgcgatcat gcaatggacg aatattcatt gataactctc ctttgagaaa gtccgtatct
     3601 gttatgggtg acgccggagc ttacgtggtt tcccggctgg tgaccagaga aatggggatg
     3661 aggattcgcc ccttcaaggg ggaaaaagaa aaaaaattct gatcagcaca aaatcgggtg
     3721 aaaaccctga ttcacctcac atttcatcgc aatttcttca tcgccgtcat aagcgaatct
     3781 gattgtgcta ccatcgaaaa tctacgcagt tgcccaaaat ttgggcgcaa tcggatatca
     3841 acccatgagt ggactcaaac aagaactggg gctggcccag ggcattggac tgctatcgac
     3901 gtcattatta ggcactggcg tgtttgccgt tcctgcgtta gctgcgctgg tagcgggcaa
     3961 taacagcctg tgggcgtggc ccgttttgat tatcttagtg ttcccgattg cgattgtgtt
     4021 tgcgattctg ggtcgccact atcccagtgc aggcggcgtc gcgcacttcg tcggtatggc
     4081 gtttggttcg cggcttgagc gagtcaccgg ctggctgttt ttatcggtca ttcccgtggg
     4141 tttgcctgcc gcgctacaaa ttgccgccgg gttcggccag gcgatgtttg gctggcatag
     4201 ctggcaactg ttgttggcag aactcggtac gctggcgttg gtgtggtata tcggtactcg
     4261 cggtgccagt tccagtgcaa atctacaaac cgttattgcc ggacttatcg tcgcactgat
     4321 tgtcgctatc tggtgggcgg gcgatatcaa acctgcgagt atccccttcc ccgcgccagg
     4381 aaatatcgaa cttaccgggt tatttgctgc gttatcagtg atgttctggt gttttgtcgg
     4441 tctggaagca tttgcccatc ttgcctcgga atttaaaaat ccagaacgtg attttcctcg
     4501 tgctttgatg attggtctgc tgctggcagg attagtctac tggggctgta cggtagtcgt
     4561 cttacacttc gacgcctatg gtgaacaaat ggccgcggca gcatcgcttc caaaaattgt
     4621 agtgcaactg ttcggtgtag gagcgttatg gattgcctgc gtaattggct atctggcctg
     4681 ctttgccagt ctcaacattt atatacagag cttcgcccgc ctggtctggt cgcaggcgca
     4741 acataatcct gaccactacc tggcacgcct ctcttctcgc catatcccga ataatgccct
     4801 caatgcggtg ctcggctgct gcgtggtgag cactttggtg attcatgctt tagagatcaa
     4861 tctggacgct cttattattt atgccaatgg catctttatt atgatttatc tgttatgcat
     4921 gctggcaggc tgtaaattat tgcaaggacg ttatcgacta ctggcggtgg ttggcgggct
     4981 gttatgcgtt ctgttactgg caatggtcgg ctggaaaagt ctctatgcgc tgatcatgct
     5041 ggcggggtta tggctgttgc tgccaaaacg aaaaacgccg gaaaatggca taaccacata
     5101 atccggcgtt tcgacattaa tcctggcgat cgtctttatg atcaaggcgg tcgcgctcat
     5161 catcctttcg ctggtactca ccatcaaaag tattaccgcc accggtcccg gcgctaaaac
     5221 cgccgcctgg catacgagaa aagcgcaaat gcggcatcaa cttcactgtc agatgctttt
     5281 gcaccggcgg taataaaagc agaagaccga ggaagtcggt gaaaaagccc ggtaataaaa
     5341 gcagcaaacc ggcaatgatc agcgaaacac ttttaatcat ctccgccgct gggttttcac
     5401 ccgccgccat tttttgctgc atcagcacga aattcttaaa gccctggtta cgtaccagtg
     5461 acatcccgat aactgacgtg aatataacca gcacgagggt cagcaatacc cccaatacat
     5521 gggcaacctg aataaagatt gaaatctcaa tatagacata aaggaaaatg gcaataaaag
     5581 gtaaccagcg caaaggtttc tcctgtaata gcagccggta aaacccggct acctgaatgg
     5641 gttgcgaatc gcgttcagct tatattgtgg tcattagcaa aatttcaaga tgtttgcgca
     5701 actatttttg gtagtaatcc caaagcggtg atctatttca caaattaata attaaggggt
     5761 aaaaaccgac acttaaagtg atccagatta cggtagaaat cctcaagcag catatgatct
     5821 cgggtattcg gtcgatgcag gggataatcg tcggtcgaaa aacattcgaa accacatata
     5881 ttctgtgtgt ttaaagcaaa tcattggcag cttgaaaaag aaggttcaca tgtcaaacaa
     5941 cattcgtatc gaagaagatc tgttgggtac cagggaagtt ccagctgatg cctactatgg
     6001 tgttcacact ctgagagcga ttgaaaactt ctatatcagc aacaacaaaa tcagtgatat
     6061 tcctgaattt gttcgcggta tggtaatggt taaaaaagcc gcagctatgg caaacaaaga
     6121 gctgcaaacc attcctaaaa gtgtagcgaa tgccatcatt gccgcatgtg atgaagtcct
     6181 gaacaacgga aaatgcatgg atcagttccc ggtagacgtc taccagggcg gcgcaggtac
     6241 ttccgtaaac atgaacacca acgaagtgct ggccaatatc ggtctggaac tgatgggtca
     6301 ccagaaaggt gaatatcagt acctgaaccc gaacgaccat gttaacaaat gtcagtccac
     6361 taacgacgcc tacccgaccg gtttccgtat cgcagtttac tcttccctga ttaagctggt
     6421 agatgcgatt aaccaactgc gtgaaggctt tgaacgtaaa gctgtcgaat tccaggacat
     6481 cctgaaaatg ggtcgtaccc agctgcagga cgcagtaccg atgaccctcg gtcaggaatt
     6541 ccgcgctttc agcatcctgc tgaaagaaga agtgaaaaat atccaacgta ccgctgaact
     6601 gctgctggaa gttaaccttg gcgcaacagc aatcggtact ggtctgaaca cgccgaaaga
     6661 gtactctccg ctggcagtga aaaaactggc tgaagtcact ggcttcccat gcgtaccggc
     6721 tgaagacctg atcgaagcga cctctgactg cggcgcttat gttatggttc acggcgcgct
     6781 gaaacgcctg gctgtgaaga tgtccaaaat ctgtaacgac ctgcgcttgc tctcttctgg
     6841 tccacgtgcc ggcctgaacg agatcaacct gccggaactg caggcgggct cttccatcat
     6901 gccagctaaa gtaaacccgg ttgttccgga agtggttaac caggtatgct tcaaagtcat
     6961 cggtaacgac accactgtta ccatggcagc agaagcaggt cagctgcagt tgaacgttat
     7021 ggagccggtc attggccagg ctatgttcga atccgttcac attctgacca acgcttgcta
     7081 caacctgctg gaaaaatgca ttaacggcat cactgctaac aaagaagtgt gcgaaggtta
     7141 cgtttacaac tctatcggta tcgttactta cctgaacccg ttcatcggtc accacaacgg
     7201 tgacatcgtg ggtaaaatct gtgccgaaac cggtaagagt gtacgtgaag tcgttctgga
     7261 acgcggtctg ttgactgaag cggaacttga cgatattttc tccgtacaga atctgatgca
     7321 cccggcttac aaagcaaaac gctatactga tgaaagcgaa cagtaatcgt acagggtagt
     7381 acaaataaag aaggcacgtc agatgacgtg ccttttttct tgtgagcagt aacttaaaaa
     7441 caacaatcta atatcaactt gttaaaaaac aaggaaggct aatatgctag ttgtagaact
     7501 catcatagtt ttgctggcga tcttcttggg cgccagattg gggggaatag gtattggttt
     7561 tgcaggcgga ttgggggtgc tggttcttgc cgctattggc gttaaacccg gtaacatccc
     7621 gttcgatgtc atttccatta tcatggcggt tatcgccgct atttctgcca tgcaggttgc
     7681 tggcggtctg gactatctgg ttcatcagac agaaaagctg ctgcgccgta acccgaaata
     7741 catcacgatc ctcgcaccta tcgtgaccta tttcctgaca atctttgctg gtactggcaa
     7801 catctctctg gcgacactgc cagttatcgc tgaagttgcg aaggaacaag gcgttaaacc
     7861 ttgccgtccg ctgtctactg cagtggtatc cgcgcagatt gcgatcaccg catcgccaat
     7921 ctcagcggca gtggtttaca tgtcttccgt gatggaaggt catggcatca gctacctcca
     7981 tctgctctcc gtggtcatcc cgtccaccct gctggcggtt ctggtgatgt cctttctggt
     8041 cactatgctg ttcaactcca aactctctga cgatccgatt tatcgcaagc gtctggaaga
     8101 gggcctggtt gaactgcgcg gtgaaaagca gattgaaatc aaatccggtg caaaaacgtc
     8161 cgtttggctg ttcctgctgg gcgtagttgg cgtggttatc tatgcaatca tcaacagccc
     8221 aagcatgggt ctggttgaaa aaccgctgat gaacaccacc aacgcaatcc tgatcatcat
     8281 gctcagcgtt gcaactctga ccaccgttat ctgtaaagtc gataccgaca acatcctcaa
     8341 ctccagcacc ttcaaagcag gtatgagcgc ctgtatttgt atcctgggtg ttgcgtggct
     8401 gggcgatact ttcgtttcca acaacatcga ctggatcaaa gataccgctg gtgaagtgat
     8461 tcagggtcat ccgtggctgc tggccgtcat cttcttcttt gcttctgctc tgctgtactc
     8521 tcaggcagca accgcaaaag cactgatgcc gatggctctg gcactgaacg tttctccgct
     8581 gaccgctgtt gcttctttcg ctgcggtgtc tggtctgttc attctgccga cctatccgac
     8641 gctggttgct gcggtacaga tggatgacac gggtactacc cgtatcggta aattcgtctt
     8701 caaccatccg ttcttcatcc cgggtactct gggtgttgcc ctggccgttt gcttcggctt
     8761 cgtgctgggt agcttcatgc tgtaatgacc aatcgcgggg cgttcacgcc ccgctttctt
     8821 tcccgccgac taacatcctt cccccgtccg ttgtatagtg acctctctct tgcggttcca
     8881 tctgttcttg cgaggtgttt atgcttgatg aaaaaagttc gaataccgcg tctgtcgtgg
     8941 tgctatgtac ggcaccagat gaagcgacag cccaggattt agccgccaaa gtgctggcgg
     9001 aaaaactggc ggcctgcgcg accttgatcc ccggcgctac ctctctctat tactgggaag
     9061 gtaagctgga gcaagaatac gaagtgcaga tgattttaaa aactaccgta tctcaccagc
     9121 aggcactgct ggaatgcctg aagtctcatc atccatatca aaccccggaa cttctggttt
     9181 tacctgttac acacggagac acagattacc tctcatggct caacgcatct ttacgctgat
     9241 cctgctactt tgcagcactt ccgtttttgc cggattattc gacgcgccgg gacgttcaca
     9301 ttttgtcccc gcggatcaag cctttgcttt tgattttcag caaaaccaac atgacctgaa
     9361 tctgacctgg cagatcaaag acggttacta cctctaccgt aaacagatcc gcattacgcc
     9421 ggaacacgcg aaaattgccg acgtgcagct gccgcaaggc gtctggcatg aagatgagtt
     9481 ttacggcaaa agcgagattt accgcgatcg gctgacgctt cccgtaacca tcaaccaggc
     9541 gagtgcggga gcgacgttaa ctgtcaccta ccagggctgt gctgatgccg gtttctgtta
     9601 tccgccagaa accaaaaccg ttccgttaag cgaagtggta gccaacaacg cagcgtcaca
     9661 gcctgtgtct gttccgcagc aagagcagca caccgcgcaa ttgccctttt ccgcgctctg
     9721 ggcgttgttg atcggtattg gtatcgcctt tacgccatgc gtgctgccaa tgtacccact
     9781 gatttctggc atcgtgctgg gtggtaaaca gcggctctcc actgccagag cattgttgct
     9841 gacctttatt tatgtgcagg ggatggcact gacttacacg gcgctgggtc tggtagttgc
     9901 cgccgcaggg ttacagttcc aggcggcgct acagcaccca tacgtgctca ttggcctcgc
     9961 catcgtcttt accttgctgg cgatgtcaat gtttggcttg tttactctac aactcccctc
    10021 ttcgctgcaa acacgtctca cgttgatgag caatcgccaa cagggcggct cacctggcgg
    10081 tgtgtttgtt atgggggcga ttgccggact gatctgttca ccatgcacca ccgcaccgct
    10141 tagcgcgatt ctgctgtata tcgcccaaag cgggaacatg tggctgggcg gcggcacgct
    10201 ttatctctat gcgttgggca tgggcctgcc gctgatgcta attaccgtct ttggtaaccg
    10261 cttgctgccg aaaagcggcc cgtggatgga acaagtcaaa accgcgtttg gttttgtgat
    10321 cctcgcactg ccggtcttcc tgctggagcg agtgattggt gatgtatggg gattacgctt
    10381 gtggtcggcg ctgggtgtcg cattctttgg ctgggccttt atcaccagcc tacaggctaa
    10441 acgcggctgg atgcgtattg tgcaaattat tctgctggca gcggcattgg ttagcgtgcg
    10501 cccacttcag gattgggcat ttggtgcgac gcataccgcg caaactcaga cgcatctcaa
    10561 ctttacacaa atcaaaacgg tagatgagtt aaatcaggcg ctcgttgaag ccaaaggcaa
    10621 accggtgatg ttagatcttt atgccgactg gtgcgtcgcc tgtaaagagt ttgagaaata
    10681 caccttcagc gacccgcagg tgcaaaaagc gttagcagac acggtcttac ttcaggccaa
    10741 cgtcactgct aacgacgcac aagatgtggc gctgttaaag catcttaatg tccttggcct
    10801 accgacaatt ctcttttttg acggacaagg ccaggagcat ccacaagcac gcgtcacggg
    10861 ctttatggat gctgaaacct tcagcgcaca tttgcgcgat cgccaaccgt gaacgacact
    10921 tgcagtggga aagacggagg aaaatagcgt gcaacgtgaa gatgtactgg gagaagccct
    10981 gaaattatta gaattacaag ggattgccaa caccacgctg gagatggttg ctgaacgtgt
    11041 ggattatcca ctggacgagc tacgccgctt ctggccagac aaagaggcaa tcctctacga
    11101 tgcgctgcgt tatcttagcc aacagataga tgtctggcgt cgtcagctga tgctggacga
    11161 aacgcaaacc gccgaacaaa agctgctggc acgttatcag gcgttatcgg agtgcgttaa
    11221 aaacaaccgc tatccgggct gtctatttat cgctgcctgt acgttttatc ccgatcctgg
    11281 ccaccctatt catcaactgg ccgatcagca aaaaagcgcg gcctacgatt tcacccacga
    11341 actgttaacc acactggaag ttgacgatcc ggcgatggta gcaaagcaga tggaactggt
    11401 gctggaaggc tgtttaagcc gaatgctggt gaatcgtagc caggcggatg tcgacactgc
    11461 acatcggctg gcggaagata tcctgcgctt cgcccgctgc cgccagggtg gtgcattgac
    11521 ctgacagaaa cacagaaaag aagcgatttg ccgcaatctt aagcagttga atcgctttta
    11581 ctgaaattag gttgacgaga tgtgcagatt acggtttaat gcgccccgtt gcccggatag
    11641 ctcagtcggt agagcagggg attgaaaatc cccgtgtcct tggttcgatt ccgagtccgg
    11701 gcaccactat tcataaggcc tcgctgcaaa gcggggcttt ttcctttctt agctacggta
    11761 gatttctggt cgttgcaaca cgttatttgt ttgctggatt tttcgccatt ttcgcaggtg
    11821 ttcgttactt ttagacacct caacatcctc tgatgtacct tttccttacc ttacagctac
    11881 ggtctttcta cggtcctcca cggttccttt ctctcagatc attcataatg aagtagaaag
    11941 cacggggaaa acggggaaat tatcacttca ccttaatccg ctggtcttaa cgtgattttg
    12001 tcgcggggaa taaggtgggg aaatccccta tagtaagcgg gaaaacctga cagcatatgt
    12061 cctccaccaa tcacatggga atcttcacca gtccgcccac ctgcagcaag atcttctttc
    12121 cctttcaatt cttaccgtta ccagaatccc ccgtcctcca cgtcatcata aaatgatata
    12181 tcagaagtaa tactacttga actaaacgta ttgcactgtc aaacctaagt ggccgcagaa
    12241 agcgaggggc cgttttacag ggatacttac agaggcatta ctcaaatagc ggtgtttaag
    12301 tcaatgggga gcaagtcatg tatttgtgta atgacaagcc tttacttcag cgcaaaggcc
    12361 cgaatcgatg gttaggctac tgtttattat tcgttgtttt agcggctgag ggagcactac
    12421 ctgatgattg actgggaact ggctgcattt tcggcatcgt tgcgccgtgt ttctcaatgc
    12481 tagagtttga tactttaacc atcagaggtg atatggcgcc atctccaata cgaactgttt
    12541 tgtaattatt tgaactcatt actaggctcc ttatttattc gcggctattg gacgaagttg
    12601 tggaaggttg ctgagtagtc cctccagatg attgttgcga agctcgggcg gtagggatcg
    12661 cctgcatctg aggaacattt gcaccatttc ctacaaatac acgctgcatg gaaggcataa
    12721 ttgccccatc catccctaag cctgagtgta ttaccttaga tttatcgttc aagttagctc
    12781 ctgacgttaa tttagttttt ggcggaatca gtagatgttg taggtgaagg agtaccgttt
    12841 actgttgcat ctgtctgatt tgtttgtgtt actggttgca tgagggggac cgtagcacct
    12901 ttatcaacag gtactctttg catcattggt ggtggcaacc catcgttatt aatttgctgc
    12961 gttttattaa ccatatcgct tttgctaacc tccgttcttc tatcaaagtt gatcacacca
    13021 aatgtaatga ccgagattat agctaaaaca aaaaaaccac ctgaaaatct attcacccaa
    13081 tccaaagcct ttgattcgag gcttactttc ccaaaaacac tttcatcttc ttcgatgtaa
    13141 tatttatgag ctatttctaa ctgagccctc agtgctgcat tactaatcaa gaaactgaat
    13201 attgttgata atattgcaca tccaaataat acccatgcag agtaaagcag ccaaatatgc
    13261 gccgtttgat tatgagggac tatatcttta aagacagtta atgagatggc aaggccagca
    13321 gatgataacg tcagtatgtt cttatcataa ttatcagtat tagaaagctc acgcttatca
    13381 acttcagctt tgtaagtagc gaaaattgat gttgcttgag ctcgctcctc ttcagtcctt
    13441 gccacttatc gattccttta gctgaactta acgaggttta ttcgactgcg acaacaatga
    13501 cgcagcatag cttttggttt cctcgctata ctttgaactt ttcataacat cggaagctac
    13561 tgtctccatc tcagcaccag tttgcttacc agtccgggct tgtgcgacaa cagaagcagc
    13621 aagcttcttc ttaatttcag atgagttttc atcttgtaga gctttcgctg cttgagaggc
    13681 gagcttaggt gaggattgct ttttgttaat agccatagaa tatctccaag agaaatacaa
    13741 tatattgaca gcttacgaac aattacaact acatatagta ttgcagaaaa aaatcgataa
    13801 caacattatt gcaaggacta atcgtgatgc agatcataga aatactgttt ctataaacag
    13861 tgctttgagc cttgaagcag gtctaatctt tgccacatac tgctgtggca ttgattcccg
    13921 ctacattcct gagggaatca gacagttagt atttggtttc gcttgtatcc agctccacta
    13981 atcatgattg gacagtgatg agacatcact agcaacaaca ggaagttaac agtctccgca
    14041 gaacaccgac cagacggtaa gactgccatc accaaatgac tgaccttttg acctgctcac
    14101 cattgattaa cacattgtga tgtcagcaat gtctgctctt gatacatggt cgactgtgag
    14161 acctgccata tacgggcaac gatcctagtc cttatggcat gtaatagtcc cagactgaaa
    14221 cacatattat ttctgtgccc ctccccctga ttgcatcttt cccctaagac cagaactgga
    14281 ccgtaaagct tgttcttact gcatttctcg tcttcattca tctcatgtgg cacctgcata
    14341 tccttcgcca cgccagaaag tcagcttagc cataaaaagt gccgtaccgg ggatgggtca
    14401 accctgacgt tctttacacc agtcacagga ggtaatatgt ccactcccaa acgcttccgt
    14461 tttactaatc agctcattaa atccctgccc cctaacacat cagatgcacg cagtaccgat
    14521 gccgaataca gcgacaccga gatttccggc ctcaagtgtc tggtcagcag aagagaaggt
    14581 cgtaaaaagt ttttgctacg ctacctttat catggacgaa aacgcgccat cgccattggt
    14641 cactggcctg aagtggacgt caatcttgct cgtcagattg ccatagaaca caaacgttcc
    14701 cttgccaccg gcactgaccc taagcaggag cgtgaaaaca agagaatgga gctgacgttc
    14761 gatgaactct ttaatcagca ttatctgcca tgggcaaaat ccgctaaacg cagttgggga
    14821 aaggatgttc agcgctaccg cgatcatatc cgtcccgcca ttggtcagag gctgctgagc
    14881 gatatcacac cagcgagcat tctgcgcctt cagcagacgt taagtacttc cctgtcagcg
    14941 gctacctgta accgtgtcat cgtactcatt aaagccgtct ttacatgggc cggaaggcaa
    15001 agcatgacct ctgttcatcc ggcaagggtg gtacagctac tcagggagaa caacgcccga
    15061 cagcgttatt ttaccgagga cgaaattcgt cgtatttttc tctccgccga taacgatacc
    15121 agccctgtag ctgcccgcta tgtcaaactg ctgttactga ccggccttcg tcgtgatgag
    15181 ctgcgtctgg ctaagtggga gcatgttgac ccggttaaac gcaccctgtg gctccctgaa
    15241 accaagaatg gctatggacg gattgttcac cttaactcac tggcgatgga cgtaatcaga
    15301 acgctgccaa cgcagcgggg aaatccgtgg ttgtttatag gcaaaaaacc ggggatgccg
    15361 ctgagtaacc cggttaaggc ctttcagcgt atcgttcgtc gggcggggat tttcgacaaa
    15421 gaagtatgta ttcatacctg tcgccattcg gttgccgccc tcatcgtgtc ctacggcgga
    15481 acactgtatg acgttcaggc tcaactgggg caccgcagca gtcagtcttc acaacggtat
    15541 gcgcatcttc atcctcagcg cttacagaac accagtcagt tactggcgga aaggataacg
    15601 gctcagctac cgtccttgtg ataacagaaa agcagaggca agtgcccctg ctttttttta
    15661 ctgttcagcc atcagtcaga acgggatggc cccatattct tcatccggca cccggttatc
    15721 atcatatctg ttatagagtt cacgagcaca ctcgcgcgcg tctttaatag acatacgagg
    15781 gaacctgccg aggggttcat aatagcaggc ccctctaagc gtataacgag ccaggaattt
    15841 aatctgaccg ttggcaatga cggcccggag cgccggctca ctgccatcga agatttcacg
    15901 agaaatgtcg tgatacaggc actccgccag tgtgttaacc agagcgtaat cattgaatga
    15961 ggttttttct tcattttcgc tgctgatagc gtgaggaaaa ccgtcatagt actggtctga
    16021 ttcaaaaatc atacatcacc ttctattcgt tgttattcag tcaaaatggt tttattcagg
    16081 gttaaaattc ggtcatcaca ttgcatttca gaaacgcttt tttgacctgg tagtaagact
    16141 gcgccatcgt cttcagcgct ctttttttat agtgtccggc actttcgcca agcagataac
    16201 cggaatgctc taacggtctg gccacatcaa cggtcttaag tcccttttca gaacagagct
    16261 tttcaaacac cgattcataa atcaggaagt cgccggtttt gtaatggacg aatccccaca
    16321 tatctcccgg gcatcgggca tgttcgtcac gcagggtgat aaagtgcctg ctgctttttg
    16381 tcaccagcgt cagctgaagc tcctccagca tgttacgcac cgggttatac tgattctggt
    16441 tatcatccag ccagcgtcgt gcagcggaca taacggattc agccaccgca tcgctttcga
    16501 aaggtaaaag gccatatttc agcgccttct ctcctgccag ccataccagc gcaaatcttt
    16561 tcagtccgcg ccgctgaacg cgggtaatat cacgcccctc atccctcagg gcctcttcca
    16621 gtgcattaca gtacgcatct gtctgttcct ggagccagga tgaaaactct gcgtaggtgc
    16681 gaagaccgcc agccgtattt tttccggata acagtttgcc gataaatgca ggcccggcat
    16741 gaccataagc ctctgccagc cccgtcttca gtgcatctgc acgggcacgt gtttctgata
    16801 ccggttcatc cggtttttga gcatcctcag gcagcagttc catactgatg gccctgtgct
    16861 cctgccctcc ctgtaacacg gctttctgtt cagccagctt ttcggagatt gacatctcac
    16921 cggtagagag aataaacatg cgccatttaa acggctcatc tgccgtcagg ccacggttca
    16981 ggcgctcttt tgccgtacct gacgtaatgt tgtaaagcag tgaggaaagt tgcttatgat
    17041 tactcgcgcc cagttcatcg acgcaggcga ccagaccgga atagtcagcg agaagacgct
    17101 ccatcgcatt cggcgtggta ttccagcgaa taatatttac attcccccca ctacccggct
    17161 cgctgccatc accatggacc gatgcagcca gcatcaggag aagcgtttta ccggtggtgg
    17221 atacgccgtg aaaatgaaac atgcccacat ccataccggc aggaaccaga agcatcgaag
    17281 caaaggacgc actcagcgca aaggtctgcg cccagccatg gacattttcc cggatgaggt
    17341 taatccactc ctccagagcc ccacctttgt gcagtccggg acgcacgcgg aatcccggca
    17401 gcagataatc aaacccggtc agagaggtca caacgggctc atgaccatgt aaaaaggcca
    17461 tatcctccga aacgaatccc attgcatctg ccatcagata gacaggcagc ggtaaactcc
    17521 ggcagctctg aataaaacgg tcaaaaagag caagctgctg tggagagggg ccaacacaaa
    17581 atccacgacc tgccagtctg cccataacct gagcaccacc ctgaacaata tcctcaaagc
    17641 tcacctgggc cttaacatac ttacccagcc cgttaccggt catcaggctg atcccaagac
    17701 cggactcctt atcttttcca caaaacaggg cgacaggcac aactggcgtg gtgcaaacct
    17761 gcacaggttt ctgtccaccg atgtagaaaa ggccatcagc cgtattcaca taatgccgtg
    17821 gcagcccatc accaataccg agtgaagcga tatcatcttc accgacctca gtgttttcag
    17881 cacgcctgat aattgcttcc gacattttgt tgaaattttt atcgcggtca gcaaaagcgc
    17941 gaatacgctt aatcccggaa accttaggaa cattaattgt cactttttta ttcatgtgta
    18001 ttaccatcaa attacgccac cgtcagaaaa cgggtgacat cggaatcctg gttaaaagcc
    18061 atgtcgttga atgcaggttg ttaccccatc gcccgattaa cgacttcgtg ataacgcgcc
    18121 ggggaagcgt gcaagtaaag ttccgttact tttatcgtgc tgtgacccag taagtctttg
    18181 acggtgtgaa tgtcggcccc gctctcaatg agctttgtcg caaaggtatg tcgcatctca
    18241 tggatggtga acggtggaat acccgcagcc tcacaaagcc gtttttgcag gcgacgtggc
    18301 tcagcaaccg gattgtcggt ccccttgccc ggaaataccc agccttcatc acccagatgc
    18361 tgacgctgtt ctgtcagaag aacgaaagcc ttatcacaca ccggtacacg gcgaagtctg
    18421 ccgtttttgg tatggcgaag agtgataacc tttcggctcc agtccacatc ctcaaggcga
    18481 agatgacggg tttcccccag acgcagtccc gtcagggcaa ggaacatgag cagagcaaga
    18541 gcttcatgat cgggattacc attacgccag cgctcacaga catccatcca gcactgaagc
    18601 tgggagtcat ccatggcgcg tctatctgac gtcacttcag gcagcgcttt gatataaagc
    18661 gccggagagc ggctgatata tcccatgcgg atagcccagg taaacaccgc tttaatgagt
    18721 gccagatggc ggttgcgggt ggcaggcttg agatcgagat catgcagata ggccacaatg
    18781 tccgcctccg tcatatcggc gattttttta tcgccaagac gagggagtag atacttgtga
    18841 attttagaca ggtctgtcct tgctgaacgc
//
LOCUS       DAPHBJ010000083        18515 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.083, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000083 DAPHBJ010000000
VERSION     DAPHBJ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.083"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            120..812
                     /locus_tag="RHZ76_002511"
     CDS             120..812
                     /locus_tag="RHZ76_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDV2813130.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
     gene            835..2262
                     /gene="mdtD"
                     /locus_tag="RHZ76_002512"
     CDS             835..2262
                     /gene="mdtD"
                     /locus_tag="RHZ76_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HDV2813131.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(2228..3220)
                     /gene="rbsR"
                     /locus_tag="RHZ76_002513"
     CDS             complement(2228..3220)
                     /gene="rbsR"
                     /locus_tag="RHZ76_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HDV2813132.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(3224..4153)
                     /gene="rbsK"
                     /locus_tag="RHZ76_002514"
     CDS             complement(3224..4153)
                     /gene="rbsK"
                     /locus_tag="RHZ76_002514"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDV2813133.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            complement(4279..5169)
                     /gene="rbsB"
                     /locus_tag="RHZ76_002515"
     CDS             complement(4279..5169)
                     /gene="rbsB"
                     /locus_tag="RHZ76_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HDV2813134.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            complement(5194..6159)
                     /gene="rbsC"
                     /locus_tag="RHZ76_002516"
     CDS             complement(5194..6159)
                     /gene="rbsC"
                     /locus_tag="RHZ76_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDV2813135.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALDGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            complement(6164..7669)
                     /gene="rbsA"
                     /locus_tag="RHZ76_002517"
     CDS             complement(6164..7669)
                     /gene="rbsA"
                     /locus_tag="RHZ76_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HDV2813136.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            complement(7677..8096)
                     /gene="rbsD"
                     /locus_tag="RHZ76_002518"
     CDS             complement(7677..8096)
                     /gene="rbsD"
                     /locus_tag="RHZ76_002518"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDV2813137.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            complement(8263..10131)
                     /gene="kup"
                     /locus_tag="RHZ76_002519"
     CDS             complement(8263..10131)
                     /gene="kup"
                     /locus_tag="RHZ76_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HDV2813138.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10354..11850
                     /gene="ravA"
                     /locus_tag="RHZ76_002520"
     CDS             10354..11850
                     /gene="ravA"
                     /locus_tag="RHZ76_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HDV2813139.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERNALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVSAQIRQAQQC"
     gene            11844..13295
                     /gene="viaA"
                     /locus_tag="RHZ76_002521"
     CDS             11844..13295
                     /gene="viaA"
                     /locus_tag="RHZ76_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HDV2813140.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSAITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(13300..14292)
                     /gene="asnA"
                     /locus_tag="RHZ76_002522"
     CDS             complement(13300..14292)
                     /gene="asnA"
                     /locus_tag="RHZ76_002522"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDV2813141.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            14444..14902
                     /gene="asnC"
                     /locus_tag="RHZ76_002523"
     CDS             14444..14902
                     /gene="asnC"
                     /locus_tag="RHZ76_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HDV2813142.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            14992..15435
                     /gene="mioC"
                     /locus_tag="RHZ76_002524"
     CDS             14992..15435
                     /gene="mioC"
                     /locus_tag="RHZ76_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HDV2813143.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKDSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            15814..17703
                     /gene="mnmG"
                     /locus_tag="RHZ76_002525"
     CDS             15814..17703
                     /gene="mnmG"
                     /locus_tag="RHZ76_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDV2813144.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            17767..18390
                     /gene="rsmG"
                     /locus_tag="RHZ76_002526"
     CDS             17767..18390
                     /gene="rsmG"
                     /locus_tag="RHZ76_002526"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDV2813145.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT         4657 a         4830 c         4569 g         4459 t
ORIGIN      
        1 tttttcagca aaagccccgt taatgtgcca taaaacaagc aatttgccgt ttttgcaacc
       61 gtaatcacac ttcctggtgg catactaagg aggaataaaa agaaaaagga agataagtaa
      121 tgcctttaag cgcacaacag ttagctgcac agaaaaacct ttcgtatgtt ctggctgaga
      181 agctggcgca acggatctta aaaggtgaat atgaacccgg caccattttg cccggtgaga
      241 ttgagctggg cgagcaattt ggagtgagtc gtacagcggt acgcgaagcg gtcaaaacgt
      301 taacggcaaa agggatggtt ttaccgcgac cgcgaattgg tactcgggtc atgccacaat
      361 cgaactggaa ttttcttgat caggaattgc ttacctggtg gatgacagaa gagaactttc
      421 atcaggtcat cgatcacttt ctggttatgc gtatctgtct ggagccgcaa gcctgcctac
      481 tggcagcaac ggttggcacc gcggaacaga aagcgcatct caatacgtta atggccgaaa
      541 tggcggcatt aaaagagaac tttcgccgtg aacgctggat cgaagtcgat atggcctggc
      601 atgagcatat ctatgaaatg agcgccaatc cgtttttgac ctcattcgcc tcgctattcc
      661 attcggttta tcacacttac ttcacgtcaa ttaccagcga cacagtgata aagctggatc
      721 tgcatcaggc gattgtcgat gcgattatcc aaagcgatgg cgacgcggca tttaaagctt
      781 gtcaggcgct gctacgctca cctgataagt gataacccga taacaggaca ttgaatgagc
      841 gataaaaaga agcgcagtat ggcgggtttg ccgtggatcg cggcgatggc cttcttcatg
      901 caggcacttg atgccactat tcttaatacc gccttacccg caatcgctca tagccttaat
      961 cgttctcctc tcgcgatgca atcagccatc atcagttata cgctgacggt ggcgatgctt
     1021 attccggtaa gcggatggct agccgatcgc ttcggtacgc gtcgcatttt tacccttgcc
     1081 gtgagtctgt tcacattggg ttctctggcc tgcgcacttt ctaattcgct accacagctg
     1141 gttgtcttcc gggttattca ggggataggc ggcgcaatga tgatgcctgt tgctcggctg
     1201 gccttactgc gcgcttatcc tcgtaatgaa cttcttccag tattgaattt tgtcgccatg
     1261 ccgggtctgg tggggccaat tttaggcccc gttcttggcg gcgtgctggt cacctgggca
     1321 acctggcact ggatattttt aatcaatatc cccataggta ttgcgggcct tctttacgcg
     1381 cgcaaacata tgcccaattt caccaccgca cgacgcagat tcgatatcac tggctttttg
     1441 ctgtttggcc tcagccttgt tctcttctca agcggaatag agctattcgg ggaaaagatt
     1501 gtcgccagct ggattgcctt gacggtaatt gtcaccagca tcgggttact gcttctctat
     1561 attctccatg cacgacgcac gccaaaccca ttaatttcat tagatttatt taaaacccgc
     1621 actttctcga tcggtatcgt aggcaatatt gcaacccgtc tggggaccgg ctgtgtaccg
     1681 ttccttatgc cattgatgtt acaggtagga tttggttatc aggcgtttat tgctggctgt
     1741 atgatggcac cgacagcgtt aggttccatt attgcaaaat cgatggttac ccaagtctta
     1801 cgtcgtctgg gctatcgcca tacgttagtg gggatcacgg tgattattgg gctaatgatc
     1861 gctcagttct ctttgcaatc accagcaatg gctatatgga tgctgatctt gccgttgttt
     1921 atattaggga tggctatgtc gacgcagttt accgcgatga ataccatcac acttgccgat
     1981 ctgaccgatg acaatgccag cagcggtaac agtgttctgg cggtcacgca gcaactgtcg
     2041 attagtttag gcgttgctgt aagtgcggcc gtccttcgcg tttatgaagg aatggaaggc
     2101 acaacgactg tcgaacaatt ccactatacg tttatcacaa tgggcattat tactgttgct
     2161 tcagcagcaa tgttcatgct tctgaaaaca accgatggta ataatttgat caaaagacag
     2221 cgtaaatcta agccgaaccg cgttccatca gaatcggagt aagttgtaat cgttgttgct
     2281 gaagggtcgg ctgggttatc cgatggatga gtacatcaat cgccagctcc cccagttcat
     2341 ctttcggttg gtggatagtg gttaatggtg gcgtcataaa gcttgccagt tcgatatcgt
     2401 catagccaat caccgcgata tcctgcggaa cctgtaactc tgcctgatat aacgcctggt
     2461 aaacgccaac agccatagcg tcatttccgg taaagacggc ctgaggacgc agcggatgtg
     2521 atagcagttg gcgcatagcg tcaaacccgc cgttaaattc aaaatcacca gtgacttcat
     2581 agccatcagg aatgttgaga cccgcacgtt tcatcgccgc ccgataacct tccaaccgca
     2641 ggcgcgccgg agttttatcc agcgggccgg taatacaggc gatacgggta tgacctttat
     2701 cgatcagata ttgcgttgct aagtctccgc ccagcaacga gttatcctga ataagatcgc
     2761 tgtcgccatc gaacggagcc cagtccatca tcacagtagg cactgtcgga taacgttgca
     2821 tgatttcacg cgaaggttga tgcgtttcgg tgcacagtaa cagcaagcca tcaacgcgtt
     2881 tttgcatcag cgtttccaga ttgcgattca tccgctgttc atcgccttcg gtattgcaaa
     2941 ggacgagact ataaccgcgt tcgaagcagc tgcgttcaac gccacgcacc agttctgaat
     3001 agaaaggatt ggtactggca gtgatcaaca tgccaatggt atgtgtttga ttgagtttga
     3061 ggctacgcgc cagagctgat ggcgcgtaat tgagttcttt aatcgccgct tcaactttgg
     3121 cggtaatcgc ttcactgacg aagcgatctt tattgataac gtgagaaact gttgaggtag
     3181 aaacgcccgc caggcgggca acatctttca ttgtagccaa gcgtcacctc tgcctgtcta
     3241 aaaatgcgtc gatctcttca cgccacggta cggaaggttg tgcgccttta cgtgttacgg
     3301 caatcgcagc ggcagcatgg gcaaaacgaa tcgcctctgg caatggtttt tcttccagca
     3361 atgccgtgat taacgcaccg ttaaaggtat ctccggcagc aatggtatcg acagcctgca
     3421 cccggaatcc aggaacgcgc tgaccttcac cattcacgct agcccataca ccacgacttc
     3481 ctaaagtaat cagtacagta cggatacctt tttcatgcag tacctgcgcc gccttcgctg
     3541 catcttcatc attttcaaca cgaataccgg tgagcttttc tgcttccgtt tcgtttggcg
     3601 taataatgtc caccagcgcc agcagttcgt caggaagttc gcgagccgga gccgggttaa
     3661 gcgcaacgat agtcttattt tgatgggcga ttttcgccgc tgccatcaca ctttcgagtg
     3721 gtgattccag ctgcattaat aatgctgacg cgttggcaat acgctcacgt tgcgcttcca
     3781 ccagcgccgg ggaaagggca gcattagcgc cggcatgaat accgatgaca ttctcacctt
     3841 cgccattaac aaaaatcagc gccacacctg ttgattcgcc tttgatcacg ctgaccggag
     3901 taatatcaat gttatcagtg gcgagctgct ggcgaacgct ctcaccaatg ctgtcatcac
     3961 ccgtacaggc aataaacgcg atattcgcac cgctacgccc agcagccaca gcctgattcg
     4021 cgcctttgcc gccaaatgca acctgatagt ggttaccggt tacggtttcg cctggagtag
     4081 gaaaagattg aagattaaga atgtggtcag cattaatgct gccaagaaca acgaggctgc
     4141 ctgcgttttg catattcggg atgtccataa aatgcgccac cgtgttaggg tggcgcgtgc
     4201 cctgcttttc tttatattac acgttcatct gtcgatgacg tatttatgtc accatcaggt
     4261 catacaacct gattaaaact actgcttaac aaccagtttc agatcaaccg gatacttagc
     4321 ctgaactttc tcgcctttca gcactttatc tgcggtttcg acgcctttcg cgccaatctg
     4381 atcgggtagc tgagcgatag tcgctgctag tttgccatca ttcaccgctt tttcgccatc
     4441 cggtgtaccg tcaaatccga cgaccatcac atccgattta ccggcagttt gcagtgcgcg
     4501 cagcgcgccc agcgccattt catcattctg cgcgaataca gcctgaacat ccggatgagc
     4561 ggtcaacagg ttctgcatta cgttcaaacc tttagtgcga tcaaaatctg ctggctggct
     4621 ggcaagaaca ttaaacttgt gagcagcaac ggcctgctgg aagccttcgc cacgttcacg
     4681 ggctgcggat gtaccagcaa tgccttgcag ctcgataact ttggcacctt cacccgcttt
     4741 cttcgcgatg taatcaccag cgattttgcc gcccagtacg ttatcagaag caatgtggct
     4801 caccacttca cctttcgttg cctggcggtc aagagtgata accgggatgt tcgcctggtt
     4861 agccatcttc acagcattac ccactgcgtc ggagtcggtc gggttaatca gcagaatttt
     4921 tgtgccgcga acggttaagt cctgcacgtt cgccagctct ttcgccgggt tgttctggga
     4981 gtccagcacc accaggttat agccaagttt atccgcctct ttctgcgcgc catctttcag
     5041 cgatacaaag aacgggttgt taagcgtgga gaccaccagc gcgatggtgt cttttgccat
     5101 cgcattcgca ctgacggtgg cgcttagcgc aacagcggaa accagggtag ccagtttttt
     5161 catgttcata ttcaagatgt cctgtagtcg ttattactgc tttttgttgt ctaccagcac
     5221 cgccagcaaa atcaccaccg ctttgacgat catctggtaa taggaggaaa cacctaacaa
     5281 attcaatcca ttattaagga agccaagaat taatgcgccg atcaacgtcc caacaatgcg
     5341 accttttccg cccgccagac tcgtaccgcc cagaaccacc gcagcaatag catccagctc
     5401 atagccagtc cccgccgtgg gttgtgcgga ggagagacgc gccacttcaa tgatcccggc
     5461 cagcgatgcc agcagaccac aaagagaata gacgatgatt ttgattttat tgacgttgat
     5521 accagaaaga cgcgttgccg cttcgttgcc gtccagcgcg tagatgtaac gccccagacg
     5581 cgtgtgatgc agcatgtacc aggccgcgag gaagacaatc cccatgatcc agactggcgt
     5641 cggtacgccc agcggacgac caataccaaa ccagccaaac agatcggcgt tctcagtaaa
     5701 gccggtattc actgggctac cgttggtata aaccatggtc acgccgcgca gtaaaagcat
     5761 cataaccagc gtagcgataa acgcctggac gcgacctttc gctacaatca ccccggttac
     5821 cgcaccaatt gcggcaccta acgcgagagc agcagcgaca gccaccagcg cattgacttc
     5881 aatgccgacg atagatgcag caactgcgcc ggtcagcgcc aacagagaac ctaccgataa
     5941 gtcgatgccc gacgtcagga tcaccagcgt catcccgacc gccataatgg cgttcactga
     6001 ggtttgctgg agaatattga ataagttatt gatggtgaaa aagttcgggc ttaacgtcga
     6061 gacaatcgcg atcagcacca gcagagcgat aagcgatttc tgctccatca gccacgcttt
     6121 cgtgaaataa cggcgaccag agacagtctg ggttgtcatt tttttactcc tgattcacgc
     6181 gattaagctt gcccacagcg gcagccatta acacttcctg ggtggcctgc tcacgagtaa
     6241 attccccgct gagatgccct tcatgcatga cgatgatgcg atcgctcatg cctaatactt
     6301 ctggcatctc cgatgacacc agaatgatgc tcaagccatc ggctttaaac tggttaatca
     6361 gttgatagat ctcttttttc gcgccgacat ctacgccacg ggtaggttca tcaaggatca
     6421 acactttggg gcgtgtcatc agaccgcggg caatcgccac tttttgctga ttgccaccgg
     6481 aaagcagacc aattgcctgt tccatcgacg gagttttcac attaaacaga cgaatgaaat
     6541 cactcacagc ctgctgttca tcggcatgct tcaaactgcc gccagcgcgg ctgaagtagc
     6601 gcagcgctgt cagcgacatg ttctctttta ctgacatgcc caacactaaa ccgtcacgtt
     6661 tacggtcttc ggagatatac acaatgccgt ttgccaagcc atcctgcggt gaacgggtaa
     6721 cgacttcatg cccatccagg gtgacgtaac cgctggtgcg cggtagtgcg ccgtagagca
     6781 ctttcatcag ttcggtacga cctgcgccca tcaaaccaga gacgccaaga atttcgcctt
     6841 tgcgtaaagt aaaagagaca tcgttaacgc caggtccgca gagattatcg actttcaggc
     6901 ggatatctcc cggcgctttg tccaggtgcg gatattgatc ttccagcttg cgacccacca
     6961 tcatctcaat cagcgaatct tcggtcagtg atgccacttc gcgctcagca ataaattgcc
     7021 catcacgaaa aacggtaacg tcatcgcaaa tctcgaagat ttctttcatg cggtgggaga
     7081 tatagacaat accgcggcct tgcgatttca gctcgcggat gacgcggaac agggattcgg
     7141 tttcggtatc ggtcagcgca tcggtcggtt catccataat gatgactttc gactcaaagc
     7201 tcagcacttt ggcgatttca accatttgct ggtcaccgat ggaaagatcg cccaccagct
     7261 tgtcgctttt aaagcgcagg ttaagtttag ccagcaattt atccgcttcg gcatacatgg
     7321 ttttccagtc aattttgcca aagcgattaa caaactcacg accgaggaaa atgttttcgg
     7381 caatggtcaa ctgcgggatc aggttcagtt cctgatggat aatcccaatc ccggcttcct
     7441 gggaagattt cggcccggta aatgtcgttt ctttccccag ccataaaagc gtgccggcat
     7501 cgcgagcata gataccagta agcactttca tcatggtgga tttacccgcg ccgttttcgc
     7561 ccaccagcgc catcacgcgg cccggataga catttaacgc tgcgcccgag agggctttta
     7621 cgcccgggaa ggctttatcg atgcctttaa gctgaagtaa tgcttccatg acggcctcag
     7681 aacgtcacgc cagcacagag aatgatattc gcatacggag aacattctcc gctgcgaatt
     7741 accgcctgac tttctgcggt ttgttgtttg aattgttcat gcgtggtgta acgaatttca
     7801 atggtatttc cctggtgttt ttgcagctgc tcaaggtgag tgagcaacgt ttcgtggagt
     7861 tgcggattat gttgtttgat ctcttccgcg ataatggcag cctcgacctg catttcattt
     7921 gtgacgacgc ccagcacctg cataaaagaa ggtacaccct gggttaatgc catatcgata
     7981 cgcgttgtac ttttggggat gggtaaacca gcatcacaca ccaccagcgt atcggtatgt
     8041 cccagacggg agatcaccga tgaaatatca gaattaagaa cggtgccttt tttcattttt
     8101 ttctccatca gcgaaacgtt tcgctgaccc gcagtttaat cctgactgag ttgggaaaac
     8161 catcgtgacg ttacggaaat gtgatcaacc tcgaaacgtt ttacatggtg attaaccatg
     8221 aaaacaaaaa cgccccctta tggagaaagg aggcgtctgg cgttagattt cgacctgagt
     8281 acccagttcg ataaccctgt ttggcggaat ttcaaattga tctggtgcac gcagcgcatt
     8341 acgttgcagc agcaagtaca gcttgccgcg cagacgcaaa taccacgggc gtttgccgag
     8401 gatcaacgac tcatgcgaca taaagaagga ggtttccatc atccggcaac ttaatccttc
     8461 cagaccgcag cggtggaaaa cttcttccac gtttggcgtt tctcgccaac cataacttgc
     8521 caccacgcgc cagaaagtgg gcgacagttg ttcaatctgt acccgacgga cattatggac
     8581 atatggcgcg tcttcggtac gcagagttaa cagaatcacc cgctcatgca ataccttgtt
     8641 atgtttaagg ttatgcatca gcgcaaaggg aatgacgttg attgcacgcg acatatacac
     8701 cgcggtcccg ggcacgcgaa caggcggtga tttctccagc gaagcaatca tcgcttccag
     8761 agagttacca tgttcatgca tccgccgcag caagcggaaa cgctcgctct tccaggtggt
     8821 catcacgata aacatcacag taccgaggct caatggcaac cagccgccgg agagcagttt
     8881 atcgaggtta gcggtgaaca atggaatatc gacacaaagg aaagcaatca ggatcagcgc
     8941 aacaaaatac ttattccagt gccagttctg acgtgccacg gtagtcgaga gaatagacgt
     9001 cagcaccatg gttccggtca ccgcaatccc gtacgccgcc gccaggttgc tggagtgctc
     9061 aaagctgaca atcacaatca cgaccgcgac atagagcatc cagttcacaa acggaatata
     9121 gatttgccct gactccattt cggaggtgtg aataatgcgc atcggcgaca aatatcccag
     9181 acgtaccgcc tgacgcgtca atgagaagac gccagagata accgcctgcg aggcaattac
     9241 cgtcgccagt gcggcgatga tcagcagcgg gatcagcgcc cagtccggtg ccaacaggaa
     9301 gaacgggttc ttaatcgctt ccgggttctt taacaacagc gctccctgac cgaagtaatt
     9361 aagggtcaag gaaggtaata cgacggtgaa ccacgccagg cgaataggga acttaccaaa
     9421 gtgccccata tcagcataca gcgcctcgac ccccgtaatc gacaacacca ctgcccctaa
     9481 tgcaataaaa gaaaccgttt tgtattcaag gaagaaatgc accgcccaca ttggattcag
     9541 cgcatgcagc acttccgggt tagcaataat gctacgtaac cccagtcctg ccagaatcaa
     9601 aaaccaggtc agcatgatcg gcgcaaacag cttaccgacc atagccgtgc catgtttttg
     9661 aatcataaat aataaagtga gaacgataat tgagagggga actatccagg tatccagctg
     9721 cggggcgacg atttccagac cttcaatggc tgacatcacc gaaatagcgg gtgttatgac
     9781 gacttcacca tagaaaaagc tgccgccgat tagccccata atcaccagca ttgatgtggt
     9841 tcgcgccgac gtattacgcc cggcaagcga catcaacgtc aggatccccc cctcaccggc
     9901 gttatctgcc cgcatcacga aggtgagata tttaatggaa accacaaaga ttagcagcca
     9961 gaagatcagc gataaaaagc caaacacggc atcgcgttca acgccaaaac caaactggcc
    10021 ggacaaacat tcacgaagtg tatataacgg gctggtacca atatcgccgt agacaactcc
    10081 aatcgccgcg agggtaatcg cgggcaatga ttgcttatta tcagtgctca tagactagtc
    10141 tttcgttgaa atatgaaatg tgtgcttagt cccttggccc acaaaaaagc gcacagtatg
    10201 cacgattaac ggcaaaatcg tactcctaaa tgcggccaca ttaacctggc tcaaagaaaa
    10261 ataccccccc tttgagacta ttttagccac cagcaagaaa cgtctatact cgcaatttac
    10321 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
    10381 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
    10441 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
    10501 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
    10561 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
    10621 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
    10681 attgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
    10741 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtctg
    10801 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
    10861 cgcatgctga ttcgtctgtg gttagataaa gtgcaggata aagcgaattt ccgctccatg
    10921 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
    10981 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
    11041 gagctgattt ttatgctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
    11101 gatcgtcgct ggaaaaaagc gatccgatta ttacaggcca gcgccttttt tagcggtcgc
    11161 agtgctgttg ccccggtaga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
    11221 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcacgc ctggcaacag
    11281 caagggatgt tgactcgcct tggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
    11341 caaagcgata aaacagcctt aacggtaatt cgtctgggcg gcattttcag ccgtcgtcag
    11401 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
    11461 ttaaaattgc atgatatgga agtggttcat atctcctttg agcgtaacgc gctggaacag
    11521 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
    11581 ttgaatctgg aagtggatag cgcccaacat cttgttgtac gcgatgtaag tttacaaggc
    11641 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
    11701 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
    11761 tgtctgttta tccctggcga ctggttaggt cgcattgaag ccagcctaca ggatgtcagt
    11821 gcacagattc gccaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
    11881 tcagcgaaga gggattgatc gaagagatga tcatcgcgct gctggcctca ccgcagctgg
    11941 cagtcttctt tgaaaaattc ccacgcctga aggcagcaat cactgatgat gttccccgct
    12001 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccagaa ctcaccgaag
    12061 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
    12121 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccgacagt
    12181 tggttgatgc taacagcgcg atcacttcag cgttacacac actttttctc cagcgttggc
    12241 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
    12301 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
    12361 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggc cagcttaaac
    12421 gtggcgacta tcagttgatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
    12481 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
    12541 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
    12601 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
    12661 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
    12721 atcgcctgca cggtgagtcg tggcgtgaaa aagtgatcga acgtccggtg gtacataaag
    12781 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
    12841 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc cttgatgcgc attgctctcg
    12901 cagaaaaccg gcgctgctat attatgctat tttccaccga gatcgtccgt tatgagcttt
    12961 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
    13021 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
    13081 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
    13141 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
    13201 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
    13261 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
    13321 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
    13381 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
    13441 gcgcagcagc gcctgatgcc actccagctc caggcgatct tcgtcaccgg tcagcgccag
    13501 ttgatgcttc agcgtgtcgg catctacacg gatccccatg gaggaaagct caaacgcatc
    13561 ttccagtacc gggttccaca ccagaatatc gccgttcaga cccgcatggc ccagctctga
    13621 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tggcgatgac catcgctcag
    13681 cttgccgcca atcccgacaa ggaataccgc gccaagatct ttcgctatcg cccgctcacg
    13741 ccctttggca tcaagatccg gataacgaga cagtaactcc tggctgtgta cgaagtggat
    13801 ctgatccggc aggaacggtg ccaggccaaa ctcttcgcta accgcagctt cggttgcttt
    13861 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagaattgac gctcaccgtc
    13921 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
    13981 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
    14041 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
    14101 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
    14161 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
    14221 ttccagttga cgagaaaagt gagatttcac gaagctaatt tgacgttgtt tggcaatgta
    14281 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
    14341 acacacatgc aataatcatt caataaaaag cctaactcac ttttgattca ttaaaaaaga
    14401 aggctaaaat agaatgaatc atcaatccgc ataagaaaat cctatggaaa attatctgat
    14461 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
    14521 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
    14581 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
    14641 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
    14701 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
    14761 catctttata aaagtgatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
    14821 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
    14881 catgcgtacc atcaagccat aatcggcttt tttaatccca tacttttcca caggtagatc
    14941 ccaacgcgtt cacggcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
    15001 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
    15061 gaaaagctgg aagaggcggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
    15121 ttatctgcct cggggatctg gctgattatc agctccaccc acggtgccgg agatattccg
    15181 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
    15241 cgctttggcg caatcggtat tggtagtcgt gaatatgaca ccttttgtgg ggctatcgat
    15301 aaactcgagg cagaactcaa agattccggt gcaaaacaga caggcgaaac actgaagatc
    15361 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
    15421 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
    15481 ttggatcaac cggtagttat ccaaggaaca acggttgttc agtttttgag ttgtgtataa
    15541 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
    15601 cctttccagg ttgttgatct taaaagccgg atccttgtta tccacagggc agtgcgatcc
    15661 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
    15721 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
    15781 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
    15841 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggccgcggc gcgtatgggt
    15901 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
    15961 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
    16021 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
    16081 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc tctaccgtca ggcggtacgt
    16141 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
    16201 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
    16261 gccgtcgtgc tcaccgttgg gacattcctc gacggtaaaa ttcatatcgg tctggataat
    16321 tacagcggtg gccgtgctgg tgatccgccg tccattccgc tttctcgccg tttgcgtgaa
    16381 ctgccgctgc gcgttggtcg tctgaaaacc gggacaccac cgcgtattga tgctcgaact
    16441 atcgacttta gtgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
    16501 atgggcaatg cgtcccagca tccccagcag gtgccgtgtt atatcactca taccaacgag
    16561 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
    16621 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgccgac
    16681 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaacga aatttatccg
    16741 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
    16801 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
    16861 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
    16921 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttact ggccggtctt
    16981 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcgcgttc tcaggcgtat
    17041 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
    17101 acctcgcgcg cagaatatcg tctgatgctg cgcgaagata acgcggatct gcgtttgact
    17161 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
    17221 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
    17281 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
    17341 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
    17401 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
    17461 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
    17521 ctacccgcga cactggatta ccgccaggta tccggtcttt ctaacgaagt gatcgccaaa
    17581 cttaacgatc acaaaccggc ctctatcggc caggcttcac gtatttctgg cgtcacgcct
    17641 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
    17701 taacacatta aaaatgtctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
    17761 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttaccgat
    17821 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
    17881 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
    17941 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
    18001 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
    18061 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
    18121 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
    18181 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
    18241 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
    18301 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
    18361 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
    18421 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
    18481 acgttcactc ttttgcatca acaagataac gtggc
//
LOCUS       DAPHBJ010000084        18453 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.084, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000084 DAPHBJ010000000
VERSION     DAPHBJ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.084"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            55..1275
                     /locus_tag="RHZ76_002527"
     CDS             55..1275
                     /locus_tag="RHZ76_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDV2813146.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPLQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKSKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1248..1877
                     /locus_tag="RHZ76_002528"
     CDS             1248..1877
                     /locus_tag="RHZ76_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDV2813147.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1878..3038)
                     /gene="ybdL"
                     /locus_tag="RHZ76_002529"
     CDS             complement(1878..3038)
                     /gene="ybdL"
                     /locus_tag="RHZ76_002529"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HDV2813148.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAHHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDANSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLT
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3147..4235
                     /gene="hcxA"
                     /locus_tag="RHZ76_002530"
     CDS             3147..4235
                     /gene="hcxA"
                     /locus_tag="RHZ76_002530"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HDV2813149.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFKKVESFKA"
     gene            complement(4245..4442)
                     /locus_tag="RHZ76_002531"
     CDS             complement(4245..4442)
                     /locus_tag="RHZ76_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDV2813150.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4625..6730)
                     /gene="cstA"
                     /locus_tag="RHZ76_002532"
     CDS             complement(4625..6730)
                     /gene="cstA"
                     /locus_tag="RHZ76_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HDV2813151.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6911..7324)
                     /gene="entH"
                     /locus_tag="RHZ76_002533"
     CDS             complement(6911..7324)
                     /gene="entH"
                     /locus_tag="RHZ76_002533"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HDV2813152.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7327..8073)
                     /gene="entA"
                     /locus_tag="RHZ76_002534"
     CDS             complement(7327..8073)
                     /gene="entA"
                     /locus_tag="RHZ76_002534"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HDV2813153.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDALVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8073..8930)
                     /gene="entB"
                     /locus_tag="RHZ76_002535"
     CDS             complement(8073..8930)
                     /gene="entB"
                     /locus_tag="RHZ76_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HDV2813154.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8944..10554)
                     /gene="entE"
                     /locus_tag="RHZ76_002536"
     CDS             complement(8944..10554)
                     /gene="entE"
                     /locus_tag="RHZ76_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HDV2813155.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALIEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10564..11739)
                     /gene="entC"
                     /locus_tag="RHZ76_002537"
     CDS             complement(10564..11739)
                     /gene="entC"
                     /locus_tag="RHZ76_002537"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HDV2813156.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12114..13070
                     /gene="fepB"
                     /locus_tag="RHZ76_002538"
     CDS             12114..13070
                     /gene="fepB"
                     /locus_tag="RHZ76_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HDV2813157.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLDRLKALF"
     gene            complement(13074..14324)
                     /gene="entS"
                     /locus_tag="RHZ76_002539"
     CDS             complement(13074..14324)
                     /gene="entS"
                     /locus_tag="RHZ76_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HDV2813158.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRHFRQTPPQVTASDS"
     gene            14435..15439
                     /gene="fepD"
                     /locus_tag="RHZ76_002540"
     CDS             14435..15439
                     /gene="fepD"
                     /locus_tag="RHZ76_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HDV2813159.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15436..16428
                     /gene="fepG"
                     /locus_tag="RHZ76_002541"
     CDS             15436..16428
                     /gene="fepG"
                     /locus_tag="RHZ76_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HDV2813160.1"
                     /translation="MIYVSRRLLITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALSAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16425..17240
                     /gene="fepC"
                     /locus_tag="RHZ76_002542"
     CDS             16425..17240
                     /gene="fepC"
                     /locus_tag="RHZ76_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HDV2813161.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17237..18370)
                     /gene="fepE"
                     /locus_tag="RHZ76_002543"
     CDS             complement(17237..18370)
                     /gene="fepE"
                     /locus_tag="RHZ76_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HDV2813162.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKSFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISTLVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKAHVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGGVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4328 a         5225 c         4777 g         4123 t
ORIGIN      
        1 aattaacaga cttatctcac tgatcaccct gtaacgttca gagagcgtct tcagatgtct
       61 atttataaaa ttcctcttcc gctcaatatt ctggaagctg cacgagagcg tattacctgg
      121 acactcaata ccctaccccg cgtttgtgtc tcattttctg gcggcaaaga ctctggttta
      181 atgctgcatc tgacagccga acttgcccga caaatgggca aaaaaatctg cgttttgttt
      241 atcgactggg aggcgcaatt ctcttgcacc attaactatg ttcagtccct gcgcgagttg
      301 tacaccgatg tcatcgaaga gttttactgg gttgcgctcc cgcttacgac gcaaaattcc
      361 ctttcacaat accaacccga atggcagtgc tgggaacctg atgtcgaatg ggtacgtcaa
      421 cctctccaag atgcgataac cgaccctgac tttttctcct tttaccagcc aggcatgacc
      481 ttcgaacaat ttgtacgtga gtttgccgaa tggttttcac aaaaacgtcc ggcggcgatg
      541 atgatcggca tccgtgcgga tgagtcctac aaccgttttg tcgccatcgc cagtttaaat
      601 aaacaacgtt ttgccgacga taaaccctgg actacggccg caccaggcgg tcatagctgg
      661 tacatttacc ccatttacga ctggaaagtg gctgatatct ggacctggta tgcaaatcat
      721 caaagtctct gcaacccact gtataaccta atgtatcagg caggcgttcc tctgcgtcat
      781 atgcgaattt gcgaaccttt tggcccggag caacgacaag gattatggct ctatcacgtt
      841 atcgaaccgg atcgctgggc tgctatgtgc gcacgagtca gcggggtaaa aagtggcggc
      901 atttacgccg gacatgacaa tcatttctat gggcatcgga aaatcctcaa gccagaacat
      961 ttagactggc aagaatatgc attattgctg ctcaatagca tgccggaaaa aacagctgag
     1021 cattaccgca ataaaatcgc catttatttg cactggtatc agaaaaaagg catcgaggtt
     1081 ccacaaaccc agcaagggga cattggcgcg aaagatatcc cctcctggcg gcggatatgc
     1141 aaagttttac tcaataacga ttactggtgt cgggcattat catttagccc cacaaaatcg
     1201 aagaactatc agcgttataa cgaacggata aaaggaaaac gtcaggaatg ggggatacta
     1261 tgcaacaacg attaacgcag gatttaacgc aatttctcgc cagcttgccg gaagatgatc
     1321 gcatcaaagc gattaatgaa atccgcatgg cgatccatca ggttagcccc tttcgtgaag
     1381 agccagtgga ttgcgttctg tgggttaaaa acagccaact tatgcctaat gattacaacc
     1441 caaacaacgt ggcaccaccc gaaaaaaaac tgctgcaaaa atccattgaa atagatggtt
     1501 ttacccaacc cattgtggtc acacatacgg ataaaaatgc catggaaatc gtggatggtt
     1561 ttcaccgcca tgaaattggt aaaggctcat catcattaaa attacgctta aaaggttatt
     1621 tgccggtgac ctgtctggaa ggtacgcgta atcaacgcat cgccgcgact attcgtcata
     1681 atcgcgctcg tggacgccat caaatcaccg ccatgtcgga aatcgtccgt gaacttagcc
     1741 agttgggatg ggacgataat aaaattggca aagagctggg aatggacagc gacgaagtgc
     1801 tgcgcctgaa gcaaattaac ggcctgcaag agttgtttgc cgaccgtcaa tattcccgcg
     1861 cctggacggt taaatagcta aagctggcgc aggcgttcag ctgctgccag caacgtcgat
     1921 tccttcttgg caaaacagag acgaatcagt ttatgtggga agggatcggc gcaaaacacc
     1981 gacagcggaa tcgccgctac gccgtgctcc tgcgtcagcc actggcaaaa ctcaacatca
     2041 tccagggtag aaaccgcgct gtaatccacc agcaaaaagt atgtaccttc acacggtaaa
     2101 atctccagcc ggctttcatt taaagcattc accagaatat cgcgcttctg gcgataaaag
     2161 tccggtaacg caagataatg ctcaggttct gcacgtagca tatcagcaag cgtcagctgt
     2221 gccggggtat tcaccgaaaa ggtcagatac tgatgtacct tgcgaatttc ggcgctgatg
     2281 ggcgctggcg caacacaata acccactttc cagccggtca tatgataggt cttgccaaat
     2341 gaagaaaccg ccactgcccg ctcacgcagc tgcggatgcg ccagcacact ggcatggccc
     2401 tgttgtgaaa agttgatgtg ctcgtagact tcatcgctaa tgacaaaaat ctcgtgcccg
     2461 gcgatcgcct gccacaaagc ggcgaaatca gcctgctgcc agacagttgc actggggtta
     2521 tgcggagtgt tgaggatcac cagtctggtg cgctcgctta ataatgcggc aaattcctgc
     2581 cagtcaacgc gaaaatgcgg tggttgcagt gccatacgct tcactattcc cccagaaagc
     2641 gcgatggcgg gggcgtaact gtcatagctg ggatcaaaac aaatcacttc atcgccattg
     2701 cgcaccagtg cggtaatcgc cgcgtataac gcttccgtcg cccctgccgt tacggtgata
     2761 tcgctattgg catctggttg atagccatac aaacgttccg ttttctgagc aatcgcctcg
     2821 cgcaaggcct gcacgccggt catgggcgcg tattggtttg ccccctgtgc aacgtggtgc
     2881 gccagccgct cctgtaaata gcgcggacca tcaaaatcag gaaagccttg cgacaggtta
     2941 atcgcctggt gttgctgcgc cagcgcgctc atctgggtga aaatagtggt gccaagttgt
     3001 ggaagtttgc tttgtggaat cagagggtta tttgtcattt ttattgtacc tgacgaagtt
     3061 gcgtgttgaa ggcactataa cacgatgcta gtatttggca atcaagacgt ttagatgtct
     3121 aaataaaaca ataaggacaa cacaacatgc ctcacaatcc tatccgcgtg gtcgtcggcc
     3181 cggctaacta cttttcacat ccaggaagtt tcaatcacct gcacgatttt ttcactgatg
     3241 aacaactttc tcgcgcggtg tggatctacg gcaaacgcgc cattgctgcg gcgcaaacca
     3301 aacttccgcc agcgtttgga ctgccagggg caaagcatat tttgtttcgc ggtcattgca
     3361 gcgaaagcga tgtacaacaa ctggcggctg agtccggtga cgaccgcagc gtggtgattg
     3421 gcgtcggtgg cggtgcactg ctcgacaccg cgaaagccct cgcccgccgt ctcggtctgc
     3481 cgtttgttgc cgttccgacg atcgccgcca cctgcgccgc ctggacaccg ctctccgtct
     3541 ggtataatga tgccggacag gcgctgcatt atgagatttt cgacgacgcc aattttatgg
     3601 tgctggtgga accggagatt atcctcaatg caccgcaaca atatctgctg gcggggatcg
     3661 gtgacacgct ggcgaaatgg tatgaagcgg tggtgctggc tccgcaacca gaaacgttgc
     3721 cgctaaccgt gcgactgggg atcaataatg cgcaagccat tcgcgacgtc ttgttaaaca
     3781 gtagcgaaca ggcgctgagc gatcagcaaa atcaacagtt aacgcaatca ttttgcgatg
     3841 tggtggatgc tattattgct ggtggtggga tggttggtgg tctgggcgat cgttttacgc
     3901 gtgtggcggc agctcatgcc gtgcataacg gtctgaccgt gctgccgcaa accgagaagt
     3961 ttctccacgg caccaaagtc gcctacggaa ttctggtgca aagcgccttg ctgggtcagg
     4021 atgatgtgct ggcgcaatta actggagcgt atcagcgttt tcatctgccg actacactgg
     4081 cggagctgga agtggatatc aataatcagg cggagatcga caaagtgatt gcccacaccc
     4141 tgcgtccggt ggagtccatt cattacctgc cagtcacgct gacaccagat acgttgcgtg
     4201 cagcgttcaa aaaagtggaa tcgtttaaag cctgacgtac aaatttagca gcagcgcgcg
     4261 ccgccttttc caccgtagcg cgcgtcctgc cgctcacgga aaaactcttc gtaggtcatc
     4321 ggcgtttgat cgggatggtt aacccgcata tgttcgacat agttgtcgta atcaggcata
     4381 ccaatcatca gcttcgccgc ctgacctaaa tattttccgg cttttgccag tgaatcaaac
     4441 atcttggcct ccgctaacca tgttgctgaa agagtgccgg atgcggcgtg aacgccttat
     4501 ccggcctaca aatgctatca acgcgctcgg tcagtcccct cgccccaccg gggagagggt
     4561 tagggtgaga gggaaccctg ggcactaagc caacattcgc caacatcccc ccctcactct
     4621 gactttagtg tgcgcctttt gcctgcgcca cgatctcctc gacattttcc ggcattggtt
     4681 catacggcgt ttctttcgcc gttggcttcg gatctttcaa tgccgccagt gccgtcttaa
     4741 tcgagaacag tgccagaacc accacgacca ccataaagaa gatggttaac ccggcatcca
     4801 gacggttgtt gaacaccagt tgcgccagct gtgactcagt atactgcgac ggaatattgc
     4861 cgctgtcgat cattgcctgg aacttattag caatggccag gaagccgact ttcgcatccg
     4921 ggctaaacgc tttctgccag cctgcggtca gggtacaaat cagcagccag gccgttggta
     4981 ccagcgccac ccaggcgtaa cgttgacgtt tcatcttgaa caacaccacg gcacagagca
     5041 tcagcgccat ccctgccagc atctggttgg caataccaaa cagcggccac agagtgttaa
     5101 tgccgcccag cggatcgacc acgccctgat ggaggaagta gccccacgcc agtacgcaca
     5161 gcgctgttgc cagcaggtta gcaggcagtg aatcggtccg cttcaggcca ggagacacca
     5221 cgcccagcag atcctgcaac ataaagcgcg cagcacgcgt acctgcatcc accgccgtca
     5281 gaataaacag tgcttcaaac aaaatggcga agtgatacca gaacgccaca tccatcatgc
     5341 cgcccagcgc gccgtgcaga atgtaggcca tccccaccgc cagcgtcggc gcaccgcccg
     5401 cacgggaaat gatcgactgt tcacccactt cgctggcaat ctggtttagc gtatctggtg
     5461 taatgctaaa gccccagcta ctcaccacct gcgcggcaga agcgaccaca tccgccgtcc
     5521 ctgccggagc cagcaccgcc atcgggctgt tcatggcaaa atacacgccc ggatcgatga
     5581 tacaggcgga aaccagcgcc ataatcgcca cgaaggattc cattaacatc ccaccgtagc
     5641 cgataaagca cgcctgccct tcgttcgcca gcatcttcgg cgtggtccca gaagagatca
     5701 gcgcatggaa gccagacacc gcgccacagg cgatggtgat aaacaggaac gggaacaggt
     5761 taccggtcca taccgggcca gtgccatcga caaatttggt cagcgcaggc atggtcagcg
     5821 tcgggcgcat aatcaaaatg cctaccgcca gaccgacgat agtcccgatt ttcaggaagg
     5881 tagagaggta gtcacgcggg gccagcagta accacaccgg cagcaccgcc gccacaaaac
     5941 cgtaacccac cagcatccag gtcaactgca cgccggtaaa gtcaaagtac ggtgcccagg
     6001 tcggactttc tgccacccag ccgccagaga taatggcgaa aatcaggaat accagaccga
     6061 tgaccgacac ttcgccaata cgccccggac gcagatagcg caggtagatc cccataaaca
     6121 gcgccagcgg aatggtgaac gcaacggtat atgttcccca cgggctatga gtcagggctt
     6181 tcaccacgat catcgccagc actgcaagga taatgaccat gatcataaag caggccacca
     6241 gcgcaatcac cccggcggtt ggccccatct cttctttgac cagctcaccc agcgagcgac
     6301 cgtcacggcg cgtagaaaca aacagcacca tgaaatcctg caccgcaccg gcgagaacca
     6361 ccccagcgag cagccagatc atccccggca ggtagcccat ttgcgccgcc agtaccggcc
     6421 ccaccagcgg acctgctccg gcaatggccg caaaatggtg accgaacagc actttcttgt
     6481 ccgtcggcac atagtccagc ccgtcgttat ggcgcaccgc tggcgtcata cgcgtcgggt
     6541 caaccgccag cacatttttg gcgatataca gcccataaaa acggtaagcg atcagataga
     6601 tacagaccga cgccaccaca atccacagcg cgttgatctg ttccccacga tttaaagcaa
     6661 tgtatcccag agcaaatgct cccattacag agagcactgt ccagacgagg tatttccctg
     6721 atttgttcat agttgttatc cgtgtgcgtg tccatagaga tgttacattt tgtttctata
     6781 acatctttgt acaatttaac aacacagcaa ccatgtaaat cggagttgaa accaatattt
     6841 aacttaacaa tgttaactcg atcactccgt taaccgagtt ttcctgcaat ctcatctaat
     6901 tctgtcgggt tcatcccaaa actgccgtac ccagccgaca agtgcagcaa cgccgcccct
     6961 gttcatcgaa aacgacgatt tcccagctct gattttgccg accaagatgc agcggctggc
     7021 agacgccgcg tacctttccc tcagacaccg ggcgatggtg tgttgcatta agttctgtgc
     7081 ctaccacaca ctgtccgtcg cgggtcatca taaatccggc catcgatccc agcgtttccg
     7141 ccagcgccgc cgacgcgccg ccatgtagta aaccgaacgg ctgatgagta cgggtatcaa
     7201 ccggcatttc ggcttccagc acatcatcgc ccagacgggt atacacaatt cccagatgcg
     7261 ccaccattgt gttatcgctg gtggcgttca gttcgtcgag cgttaaatgg cgtttccaga
     7321 tcatgcttat gcccccagcg ttgagccgcc atcgaccaca atatcctgta gggtaatatg
     7381 gctggcgagg tcagaggcga ggaacaaaat cgtgttggcg atctcttgtg gacgggcgat
     7441 tttccccagc ggaatgccga gtttaaactg ctcgccaaag ccgcgaatac gctgttcttc
     7501 ggcgtcatcg ctcacccaca gcgtgcgttg catatcggtg tcggtggagc caggcgaaac
     7561 cacattacag cgcacgccgc tacccgccag ttccagcccg acgctcaacg ccaggctttt
     7621 cagcgccgct ttcgatgcgc cataagcact catgccaata cgcggcgtgt gcgcggcgtc
     7681 ggacgccaca gtgacaatcg ccccgccccg ctgacggcga aactggttca tggtttgctg
     7741 gaacaggtta aacgcaccgc cgacgttaac cgcaaaagtc tgctgccagt cctctttact
     7801 gagctgatcg gtcgcgccca tgcgtaaaat tcccgccgca ttgaccagcg cgtccagtcg
     7861 ctccgtttca gctaacagtc gctgacacac ttgcgcgacc tgcgcagcgt cggcaacatc
     7921 catcacttcg gtcgcaaagg gatattgctc ctgagtgaac gcttgatcaa aacctgtaac
     7981 tttcgctccc gcctcaacaa acgccagcgc cgtggcgtag ccgatacctt tacctgcgcc
     8041 ggttacccag acatttttac cgctgaaatc cattatttca cctcgcggga gagtagcttc
     8101 caccaggcgt cgatggtcgg gtttttcgcc agcatgacaa agtcgatatc accatgcact
     8161 ttgcgccagc gcgccgccag cgccatcatg cgcaccgaat ccagaccgta gtcgatcagg
     8221 ttgtcgtcat cgaacggttc atcggactcg tccagcaacg gcaggatcac ctcacgcagc
     8281 gccgctttgc tggcggggat aggtgctggc agtaattctt cagtcatcac cacccggcca
     8341 gaacgtccgg ccacatattt cagcgacatc aaatgctcgt cacggctgaa atcggccagc
     8401 gcatccgcca ccataaacgg tttaatatcg cgcataaatg cgtcggttgc ggtggtcata
     8461 cagccaatgt gggcatatac cccggtaata atcagctggt tacgtccact ctctttcagc
     8521 atttgctcca gcggagaacg atgaaacgcg ctgtagcgcc acttcaccag caccgtgtcg
     8581 tcggcatctg gcgtcaggcg atccaccacc ttttgctgtt ccggcgagcg ggtcaggccc
     8641 ggcccccaca tatcattcaa cagcgcccga tcttcatcgc tctgctcttt cggctgggcg
     8701 gtgtaataaa ccgggatatt gtgctgtttg cagtagtcgc gcagcgcagc aatattcgcg
     8761 atcacctgct ccatcatcgg gcagttctcg ccccagaagc tgacaaaata gtcctgcata
     8821 tcatggatta acaacgcggc acgttgcggt tcaaaggccc agtcaacttt attctgcgga
     8881 atatcgtgag actccggcag tgcgtaagcc tgtaattttg gaatagccat cgtgttctct
     8941 ccttcaggct gatgcgcgtg acgccagcca ctgacgtaat tgttttttat cgactttccc
     9001 gaccgccgtc agcggaagtg aatccacaca ctccacgcga tccggtaatt taaattcggc
     9061 aataccctgt tcacgcagga aacgacgcac ctgcaccgcg cgcagcggct cttttaccac
     9121 cagataagcg cagctttttt cgcccatcag ctcatcttcc atgctcacca gtgcggcgta
     9181 gatcaccgcc gggtggcgca gcagcaggtt ttcgatctct tcggcagcga tcttctcgcc
     9241 gccacggtta atctgatctt tctcgcgccc ctgcacggtg atgtaaccct ctggatcaat
     9301 agagatcaga tcgccggaac agtaaaaacc gttggcatca aaggcgctgg cattgtgctg
     9361 tggacttttg taatagccgc ggaaggtgta cggcccgcgc gtcatcaggc gtccgacttc
     9421 cccttgcggc agtggatttc cttcggcatc ggcaacccat acttcgtcat ccggacacat
     9481 tgggtaaccc tgggtatgga taattttctc cgcgctatca tcaagtcggg tgtagttcac
     9541 cagcccttcc gccatgccaa acacctgctg caactgacag ccaatctcag cgggaatacg
     9601 cgccgcaagg gtggcagaaa gacgtgcgcc gccgacctgt aacagtttca gcgaggcaag
     9661 ctgcgcccgg ctttcgcctt cgatcagcgc ctgcaaccac aggctgactg cgggtggcac
     9721 cagcgcggta acgttaacct gatgtttttc aatcaatggg aaacagagcg tggcgctggg
     9781 atcggccgcc agaacaaccg ttcctccggc aagaaagacg cccagcgatc ctggcgaact
     9841 catggcgtag ttatgagccg ccgggatcgc gcacaggtag cgtgtctgtt gtgtgaactg
     9901 acaaatctcg acgctacgac gcacgctgta gtagtagtcg ttatgagtgc gcgggatcag
     9961 tttcggtgtg ccggtggtgc caccggaaag ctggaaatag gccacttcat cagcaggtga
    10021 tggcgtggca gtaaaatcct cagccggatg gttaatcgca tcctgcaagt tatgctcacc
    10081 gctgtcgttg agcagttgca ccacgcgaat ggaggaatgt tctgtgacga aagtattgag
    10141 gaaatcatcc ccgctaaaca gcgcatgttg gcgatcggca atcagcaatg cgggttcaat
    10201 ctggctggca taggcgttca gttcactacg ctgatggctg aacaacgcca gcaccggcgc
    10261 aacgcccagt ttcagcagcg cgaaaaaggt aatatacaat tcagcgacgt tacccagttg
    10321 taccagcgcg gtttcaccag gtttaatgcc ctgacggcgt aaactacacg cgaggttatc
    10381 cgccgcctga ttcagctccc gataactcaa ctgtcgctcg ccgtcgataa ccgcgatgct
    10441 gtcactcgca gcatgtcgcg tcagaatgtc ggtcagcggc aaatcctgcc agtagccttt
    10501 ttcccgatag cgacgggcaa actcttccgg ccagcgggtg aatggaatgc tcatcctcgc
    10561 tccttaatgc aatccaaaaa cgttcaacat ggtagaaagt ttgacgcctg tttcgcgcca
    10621 ctcacccaac ggtgacgacg caggcacaat ccccgctccg gcaaacagac gcacctgatt
    10681 ttcccgcagc ttcgcgcagc ggatggtcac cacccattcg ccgttacctt cgctgtcaca
    10741 ccaacccaca atgccgccaa acagttcgcg gtcgaacggt tccagttcag caataacctg
    10801 ggtcgcggcc tgatgcggga aaccgctcag cgcgggggtc ggatgcagca gacaggccag
    10861 agtcagtgcg ttttcttgcg aattcgcttt accttcaaag ggagttgcga gatgccacag
    10921 cgtcggcgtg gtgatcagct gtggagaaga aggaacgtgt aactcactac tgcgttcgcg
    10981 cagtacctct ttcatcgcct gagtcaccag ttcatgttca tggcgatctt tttctgacgc
    11041 cagcagacga ttacctgctt cgcgatcgag cacttcatcc ggctgacgac gcgcggaacc
    11101 ggctaacgga atggagctaa aacgctcgcc gtctttacgt agcagcagtt ccgggctggc
    11161 ccccagcagg acgccaccat cagccagcgg aacatggaag ttgtaactaa ccgggttttg
    11221 cgcaatcaac cgttccagca atacgccact atcaatggcg gcgtcagtgg tgatatcaat
    11281 caaccgtgac aacaccactt tgtcgacctg cggcgtggcg gtaagtgcgg cggcgcgggc
    11341 aaccatctgt tcaaacgtgg tttgctccgg aattgcctgg cgttccacca cattcagcga
    11401 ctggctgcgg gtgaaacggc gtgcggaagc ttgtttttcc tgacgggaga acgactgcca
    11461 ggattcagga atatacagcg acgaaggctg acgtggatcg aagggaatcg ccccgaccat
    11521 caccggattt ttgatgccct gcgctttggc atcggcaaac agcgcggcga gtttttgctg
    11581 gaagggactg tcgggcgaat ccccgttcac agccggttca tcgaagcggg cgaaacatcc
    11641 tgacgtcgta aaactgcggt acggcgacat aaagaaaaag cgattgggcg caagtgttgc
    11701 catggtctgc tgtacttcct cagccagtga cgtatccata tcatcctcca aaaaatgata
    11761 aaggctttaa taatgattat catttatatt ttcggtcgct aacctaaaag caaacgcgca
    11821 ctatgtcaac tcttgaggta acgcaatctg caactcgggg ttgcatctgg ttgtggttac
    11881 aatgaaaatg agaagcatta ttgatggatt cgcataagcg caatgtgatg tcctgcgccg
    11941 ttctgccccc tctcccttcc agggtgaggg ctggggtgag ggttaatgtt cgcaccagtg
    12001 ctggctgttc ccctcaccct aaccctctcc ccaaaggggc gaggggacgg attgtgcgct
    12061 ttgtcgaatt tgtcattacg cccttaacct tattaataac aggaagctga tttgtgagac
    12121 tcgccccgct ctaccgcaac gcccttctat taacaggact tttgctttca ggaatagccg
    12181 cagttcaggc cgctgactgg ccgcgtcaga ttactgacag ccgtggcaca catacactgg
    12241 aaagccagcc gcagcgtatt gtttccacca gcgtcaccct gaccggctca ctgctggcga
    12301 ttgatgcacc agtgatcgcc agcggcgcga ccacgccaaa taaccgcgtc gcggatgacc
    12361 agggcttttt acgccagtgg agcaaggtgg cgaaagaacg caaactgcaa cggctctata
    12421 tcggcgaacc gagcgccgaa gccgttgccg cgcaaatgcc ggatctgatt ttaattagcg
    12481 caactggcgg ggattcggcg ctggcgctgt atgatcagct ttccaccatc gccccgacat
    12541 taatcatcaa ttacgacgac aaaagctggc agtcgctgtt aacgcagctt ggcgaaatta
    12601 ccgggcatga gaaacaagcg gcagagcgga ttgcgcagtt tgataagcaa ctggcggcgg
    12661 cgaaagagca aatcaaatta ccgccgcagc cggtcactgc cattgtctat actgccgctg
    12721 cacacagtgc caatctctgg acgccagaat cagcacaagg gcagatgctg gaacaactcg
    12781 gctttacgct ggcgaagtta cccgcagggt taaatgccag ccaaagtcag ggtaaacgcc
    12841 atgacatcat tcagcttggt ggggaaaatc tggctgcagg gttaaatggc gagtcactat
    12901 tcctgttcgc cggtgatcag aaagatgccg atgctattta tgctaatccg ctgctcgcgc
    12961 acctgcctgc agtacaaaac aagcaggttt atgcgctggg aaccgagacg ttccgtctgg
    13021 attactacag cgccatgcaa gtgctggata ggcttaaggc gctgttttaa gcattaactg
    13081 tcggacgctg tcacctgcgg cggcgtctgg cgaaaatgtc gcaactccac cagcaccagc
    13141 aataacaaca cgccgataat caacaaacca aaaccgctcg cgcttgcgga agcaaccggt
    13201 gtcatcatcg cgcccaaacc acccagcagc gccgcgccta tggcatcgcc cgtcacgttc
    13261 tgcgccgtcc acaaaccgtt aatccgccct aacatcgctt ccggggtttg cgtttgcagc
    13321 attgtgtatt gcagcaacga gctgaccgca ctcaaccagc cgaacagcgc cagacaaacc
    13381 acgcctaaaa tccacatcgg catcaggcca aacagaccaa tggcgaggaa cgatcccagc
    13441 gtggagagca gcatcaataa ccctggtcgc gcactatgtg ccagcttccc gctggttaac
    13501 gcaccaatag ccgcgccgag cgggatcgcc gcgtagagaa aaccaatctg tgccgctgac
    13561 atctgccagt tgtcagccag cgccggatac agtacccgca ccgcgctcgc catcgtcaat
    13621 aaaccaccca gcagcgcaat cccgcccacc agcgggctgg caagcagaaa acgaaatcct
    13681 gccagtaatg atttcaacgg atgctcacgc ggctgcggtg gcggtggcaa cgccggaagg
    13741 cttaacaacg gtagcaaggt aataaacgtg cccgccgccg ccagcccgta gttccaggct
    13801 acgccaccgg tcgccagcaa taaaccgcca atcatgggcg aaatcaccga ccccagacgc
    13861 acggtcaaca tggtgatcgc cccggcctgc attaagtttt cacgccctac cagtgctggt
    13921 gtcgccgcca atagcgccgt aacgccaagc gatgcgaaaa aaccatccca taaaccaagt
    13981 aaatagattg ccagcaatga cggctccggc agcagtgcat taaggcacag tccaatgaag
    14041 ccaatgccac aggtgccgcg cgccagcaaa atcacttttt tgcgctcata gcgatccgcc
    14101 agcacaccgc cgaccatcag gccaacaaac atcgcgccgc cggtcagcgt caccgaaagc
    14161 cccacctgcc aggtggaatg tgtcatcatc tggatctgca ccggcaccgc gacgccgagc
    14221 aaacccagag acacaattga gatgaaacga gcgaggaata ctgcgcgaaa cgccgggtgc
    14281 gttttcaaca ggctgaggtt aagcagccag gattgtttat tcattacaat gccttgccat
    14341 caaatatttt aaatacctat tcttaggctg cacatgctaa catatccaaa taagatcgat
    14401 aacgataatt aatttcatta tcatggaagt tcgtatgtct ggttctgttg ccgtgacacg
    14461 cgccattgcc gtgcccggat tgctgttatt actgattatc gcgacggcat taagcctgct
    14521 cattggggca aaatcactcc ccgcttccgt agtgctggag gccttctccg gcacctgcca
    14581 gagcgccgac tgcaccatcg tgctcgacgc gcggctaccg cgtacccttg ccggtttact
    14641 ggcaggcggc gcgcttggcc ttgccggggc gttaatgcaa accctcaccc gaaacccact
    14701 tgccgacccc ggcttgcttg gcgtgaacgc cggagccagc tttgccattg tgctgggtgc
    14761 ggcgctgttt ggttactctt ccgcgcagga acaactggcg atggccttcg ccggggcgct
    14821 ggtggcctca ttgattgttg cctttaccgg cagtcagggc ggcgggcagt taagtccggt
    14881 gcgtttaacc ctggcgggcg tggcgctggc ggcggtgctg gaaggactga ccagcggcat
    14941 cgccctgctt aatcctgacg tctacgatca gttgcgtttc tggcaagccg gttcgctgga
    15001 tattcgcaat ctacatacct taaaagtggt gctgatcccg gtgctgatcg ccggagcaac
    15061 tgcgctatta ctgagtcgcg cgctgaacag tttgagcctc ggcagcgaca ccgcgacggc
    15121 gctgggcagt cgcgtggcgc gcacacagtt gattggtctg ctggcgatta ccgtgctttg
    15181 tggtagtgcg acggcagtag ttggcccgat tgcctttatt ggcctgatga tgccgcatat
    15241 ggcgcgttgg ctggtgggtg ccgatcatcg ctggtcgctg cccgtcacgc tacttgctac
    15301 ccctgccctg ctgctgtttg ccgatatcat cgggcgggtg attgtgcccg gcgaactgcg
    15361 cgtttctgtg gtcagtgcgt ttattggtgc accggtgctg atcttcctcg tgcgacgtaa
    15421 aacgcgaggt ggtgcatgat ttacgtctct cgccgattac tcatcacctg tttgctgctg
    15481 gtttccgcct gtgtggttgc aggtatctgg ggattacgca gcggtgccgt cacgctggaa
    15541 acctcgcagg tattcgccgc gctgatgggc gatgcgccgc gcagtatgac gatggtggtc
    15601 accgaatggc gtttaccacg cgtgctgatg gcgctgttga ttggcgcagc actgggcgtc
    15661 agtggcgcga tttttcagtc gctgatgcgt aacccgctcg gcagccctga cgtaatgggc
    15721 tttaacaccg gggcgtggag cggcgtgctg gtggcgatgg tgctgtttgg tcaggacctg
    15781 acggctatcg cgctgtcagc aatggtgggc ggcattgtca cttcgctgct ggtctggctg
    15841 ctcgcctggc gcaacggcat cgacaccttt cggttgatta ttatcggtat cggcgttcgc
    15901 gccatgctgg tggcctttaa tacctggctg ttgctgaaag cgtctttaga aacggcgcta
    15961 acagcaggtt tgtggaatgc cggatcgctc aacggcctga cgtgggcaaa aacctcgcct
    16021 tccgcaccca tcattatatt gatgctcatt gccgccgcct tactggtacg ccggatgcgc
    16081 ttgctggaaa tgggcgatga taccgcgtgt gcgctgggcg tcagcgtcga acgttcgcgt
    16141 ctgttaatga tgctggttgc agtggtgctt accgctgcgg caacagcgct tgccgggccg
    16201 atttccttta ttgctttagt cgcaccgcac attgcccgac gcattagcgg caccgctcgc
    16261 tgggggctaa cccaggcggc gctatgcggg gcgctgttac tgctggcggc cgatctctgc
    16321 gcccaacaac tgtttatgcc gtatcaactt ccggttggcg tcgttaccgt cagcctcggc
    16381 ggtatttacc ttatcgtctt gttaattcag gagtctcgca aaaaatgacc gaatcagtag
    16441 cccgtttgcg cggcgaacag ttaaccctgg gatatggcaa atataccgtt gcggaaaatc
    16501 tgactgtaga aatacctgat ggtcacttca cggcaattat cgggccaaat ggctgcggta
    16561 aatccacgtt actgcgtacc ttaagccgcc tgatgacgcc tgctcatggg catgtctggc
    16621 tggatggcga gcacattcaa cattacgcca gtaaagaggt tgcacgccgg attggtctgt
    16681 tggcgcaaaa tgctaccacg ccgggcgata tcaccgtgca ggagctggtg gcgcgtggac
    16741 gttatccgca tcaaccgctg tttacccgct ggcgcaaaga ggatgaagaa gcggtaacga
    16801 aagcaatgca ggccacggga ataactcatc tggcagatca aagcgtggat accctttctg
    16861 gcggacaacg ccagcgagcg tggatcgcga tggtgctggc ccaggaaacg gcaattatgc
    16921 tgctcgacga acccacgacc tggctggata tcagtcatca gattgatttg ctggagttgt
    16981 taagcgaact gaaccgcgaa aaaggctata ccctggcggc ggtgctgcac gatcttaatc
    17041 aggcctgtcg ttacgccagc catttgattg cattgcggga agggaaaatt gttgctcagg
    17101 gagcgccgaa ggagattgtc actgctgaac tgattgaacg catttatggt ctgcgctgca
    17161 tgatcattga cgatccagtt gccggaacgc cgcttgtggt gccgctcgga cgaacggcac
    17221 cttcaaccgc aaatagttaa actaagtggt ctgccatcat ggcatcctgt tttctggatg
    17281 ccatcgcata gcgcaacagc acgccaccac aagccaccat cccgccgatc aacgcggaaa
    17341 ggatcacaat aatcgcctta cccggaccgt cttttttcac tggcaatgac gggcttaact
    17401 gatatttaaa cggcgtaaaa ttcacatcgt tgacatgtgc ttttgttaat tgctcgacaa
    17461 gatactgccg attacgtaat tcaccgttca gttccgcaac gtcagtgacc gctttttcta
    17521 tttccagttt gcgttcaata ccgtctgcac cgagagaaat agaaaaatcg gggtcatctt
    17581 taacggcctg accattactg taaacgggct ttttaattcc tgccgcgttg gcaatgtcga
    17641 gtgaataatt gaggcgctga atgtttgcat caagttgatt tttcgtttta atgcgatcct
    17701 gagccagttt ttctttttca aactgggttt tgatctccag tttattacgg acgttttcta
    17761 gcgactcttt caccaccaac gtagagatat aatcgatata cccgctcaaa acggtctgcg
    17821 cctcttcact ggttggcgcg gtaaaactta gcgtccagga ggtatacagt gacggttcat
    17881 cttttttctt actggcattg tcatcaaccg ctttcatttt ttcgctcaat gcgacaattg
    17941 cgcgatgcaa atccagttcg tcgattttcg cctcttttaa ttggtccatc acataaggtg
    18001 atgaacgcag gtactcttcc agcaagctaa ccgactgaaa cttcttgata aacaggttaa
    18061 atgcttctgt acgatcaatt ttgatatcca gatccagcac acgaagctta gtgaatgatt
    18121 tctccaactc ttgccactga acaggttctg gaggcgtgac aaccgccgcg ctggtccatt
    18181 tttgcggcag gatgaaagag atcagcaagc ctgcgcaggc aaacgcaaaa acgaccgcca
    18241 tgaccgtttt tttggcccgc cataaaactg agattagatt aagtaaatca atttcattat
    18301 tactgggcga cgccagagga taatcgggaa aatgagcgtc acttccctgt ttaatattca
    18361 gtgatgacat ggggtataac ctgaaaatga gtccaatagg gggaaatctc cacggcgctc
    18421 actttaccag ttggtgataa ttatccgaag ctg
//
LOCUS       DAPHBJ010000085        18274 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.085, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000085 DAPHBJ010000000
VERSION     DAPHBJ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.085"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..578
                     /locus_tag="RHZ76_002544"
                     /pseudo
     CDS             1..578
                     /locus_tag="RHZ76_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            681..1184
                     /locus_tag="RHZ76_002545"
     CDS             681..1184
                     /locus_tag="RHZ76_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDV2813163.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1251..2708)
                     /gene="nuoN"
                     /locus_tag="RHZ76_002546"
     CDS             complement(1251..2708)
                     /gene="nuoN"
                     /locus_tag="RHZ76_002546"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HDV2813164.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQTHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2715..4244)
                     /gene="nuoM"
                     /locus_tag="RHZ76_002547"
     CDS             complement(2715..4244)
                     /gene="nuoM"
                     /locus_tag="RHZ76_002547"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HDV2813165.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4408..6249)
                     /gene="nuoL"
                     /locus_tag="RHZ76_002548"
     CDS             complement(4408..6249)
                     /gene="nuoL"
                     /locus_tag="RHZ76_002548"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HDV2813166.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQTYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6246..6548)
                     /gene="nuoK"
                     /locus_tag="RHZ76_002549"
     CDS             complement(6246..6548)
                     /gene="nuoK"
                     /locus_tag="RHZ76_002549"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HDV2813167.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6545..7099)
                     /gene="nuoJ"
                     /locus_tag="RHZ76_002550"
     CDS             complement(6545..7099)
                     /gene="nuoJ"
                     /locus_tag="RHZ76_002550"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HDV2813168.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7111..7653)
                     /gene="nuoI"
                     /locus_tag="RHZ76_002551"
     CDS             complement(7111..7653)
                     /gene="nuoI"
                     /locus_tag="RHZ76_002551"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HDV2813169.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7668..8645)
                     /gene="nuoH"
                     /locus_tag="RHZ76_002552"
     CDS             complement(7668..8645)
                     /gene="nuoH"
                     /locus_tag="RHZ76_002552"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HDV2813170.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8642..11368)
                     /gene="nuoG"
                     /locus_tag="RHZ76_002553"
     CDS             complement(8642..11368)
                     /gene="nuoG"
                     /locus_tag="RHZ76_002553"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HDV2813171.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASAIRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11421..12758)
                     /gene="nuoF"
                     /locus_tag="RHZ76_002554"
     CDS             complement(11421..12758)
                     /gene="nuoF"
                     /locus_tag="RHZ76_002554"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDV2813172.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12755..13255)
                     /gene="nuoE"
                     /locus_tag="RHZ76_002555"
     CDS             complement(12755..13255)
                     /gene="nuoE"
                     /locus_tag="RHZ76_002555"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HDV2813173.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13258..15060)
                     /gene="nuoC"
                     /locus_tag="RHZ76_002556"
     CDS             complement(13258..15060)
                     /gene="nuoC"
                     /locus_tag="RHZ76_002556"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HDV2813174.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRVRTPSF
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15154..15816)
                     /gene="nuoB"
                     /locus_tag="RHZ76_002557"
     CDS             complement(15154..15816)
                     /gene="nuoB"
                     /locus_tag="RHZ76_002557"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HDV2813175.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15832..16275)
                     /gene="nuoA"
                     /locus_tag="RHZ76_002558"
     CDS             complement(15832..16275)
                     /gene="nuoA"
                     /locus_tag="RHZ76_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HDV2813176.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16906..17844)
                     /gene="lrhA"
                     /locus_tag="RHZ76_002559"
     CDS             complement(16906..17844)
                     /gene="lrhA"
                     /locus_tag="RHZ76_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HDV2813177.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4551 a         5077 c         4730 g         3916 t
ORIGIN      
        1 accctgggtc actgcattat cgccaacgcc ccgacacgta agtcctgggc aattagaaaa
       61 aacagttcgt tatcttacac aaactgtgca accacgtaat gccggcaata ttgataatct
      121 gaataggtcc gcagagtata taaaagagtt ctttgtcagt agcggtgcca gagttaccgc
      181 gcagggcgtc cccgattggc tattacgact ctgcgcctgg aagccagaat tacccttacc
      241 cggcaatgtc ctggctttat cccgatcggg gagactttat tgctgtggtc ggcagaatgc
      301 aggatatcaa cgccgttcgt caggtaaaag cggcattgtt gtcatcccgg gatttatctg
      361 tttattctat gaatgcccca gggtttattc ccggtattga tttttctgat caccttaatt
      421 attggcaaca cgatattccc gccgtaatga ttactgacac cgctttttat cgtaataaac
      481 aataccactt gcccggtgat accgcagaca gattgaatta tcagaaaatg gctcaggtgg
      541 tggatggtgt tataaccttg ttatacaaca gtaaataata aaaatttctc gaggcgggtc
      601 cagaaacagc gacatgtaca ttttattttc cctcgtctgt tttaagatgc gaaacataaa
      661 attcaatgga ggaaaaataa atgggaatga ttggctattt tgcagaaatt gactcagaaa
      721 agattaacca actgcttgag tctactgaaa aacctttgat ggataatata catgacacgc
      781 tttcaggatt acgccgactg gatattgata aacgctggga ttttttacac tttgggctga
      841 caggcacctc tgcttttgat cccgcgaaga atgatccgct gagtcgtgcc gtgcttggtg
      901 aacacagcct cgaagatggc atagatggct tcctgggatt gacctggaat caggaattag
      961 ccgcgacaat cgaccgactg gaatcactgg atcgaagtga gctgcgtaaa cagttttcca
     1021 ttaagcgact caatgaaatg gagatttatc cgggagtgac cttttcagaa gagttagagg
     1081 ggcaactctt tgcctcgatc atgcttgata tggaaaagct gattagcgcg tatcgacgta
     1141 tgctgcgaca agggaatcat gcgctgacgg ttattgttgg gtgatggtgt cagagcgcca
     1201 aaaaaaaccg ccgaacatgt cggcggcttt ctgacttaca aactaacaga ttacatcagc
     1261 ggcattgcta accgcacaat actaatcagc ggttgtggcc atacacccag caccagtacc
     1321 aacagtgcag aaatcagcac cacgataccg cccgcgctgt actgccagtt tgacggtgca
     1381 tcgcgacccg gttgttccgg tgcgtgcagg tacaggctca ccgccacgcg caggtagtag
     1441 tagaggccga ttgccgaacc gacaaccacg gcacccacca gccaccacaa gtgtgtctgg
     1501 acaccgaccg ccagcacgta gaacttaccg ataaagccca gcgtcatcgg gataccggcc
     1561 agagacagca tcatcaccgt catcactgcc gcgaggatcg gacgatgcca gaacagaccg
     1621 cggtaagaga acagggaatc agcatccggg ccacgatacg ggctggacat caggctgacc
     1681 acgccgaacg cgccgaggct gctgaacaga taaccggcca ggtaaacccc taccgcttcc
     1741 atcgacatct cgccggtttg cagcgcaatc agcgctacca gcagatagcc gaggtgagag
     1801 atagatgagt aaccgagcag acgtttgata ttggtctggc tcagcgccat caggttaccg
     1861 aagatgatgg aggcaaaggc gataatcgcc agcaccacgc gaatcgcttc gctgtcaccc
     1921 accggtgcgt agaggaacag acgcatcacc acaccgaaga tagcgatttt gctcgccgtc
     1981 gccaggaaag tggaaaccgg cgcaggcgcg ccctggtata cgtctggcgt ccacaggtgg
     2041 aacggcacca gagagagttt gaagccgagg ccaacaatca tcaggccgaa acctgccagc
     2101 aacagcggct cgttgagcat accgtcgcca aggtttttac ccaacgcgac aaacgacagg
     2161 tcgccagact gcgcatacac cagcgccata ccaaacagca ggaaagaaga cgctgcggca
     2221 gaaaggatgg tgtatttgat actggcttcc agtgaacgtt tctggcggaa agcgtaaccg
     2281 accaggccaa acagcggcaa agagatcagt tcgataccga ggaacagaga cgccagatgg
     2341 ttggcattcg ccagcaggat cccgcccagc gcggcaatta acaccaacag gtagaactcg
     2401 tccttgttgt cgttatagcc ttcaagccac gggtaggcga aagtacaggt ggcgaggctc
     2461 gccaacaata ccagcccggt gtaaagcatg gcgaaaccat caacgcgcat cagcggcgta
     2521 acgtccatag cgcccgcctg gccaacaaac cagagcgaaa ccagcgccgc gttaagccca
     2581 ataaccgaga gcgtagcgtt gaggaaatga ttgcgtcgcc acgcaatgga gagcatcaca
     2641 accaccaccg tcaagccgac gatcagcaac ggtagcagtg cgatcaggtt ttgtggagtt
     2701 attgtcatgg cgatttacgg ccttgtagta gtaacggaat taacaaacca ctgctggata
     2761 ttgccaatcg cggagtgcga ggtatccaga atcggctgcg gatagaagcc cagcagtacc
     2821 agcagcacca ccagcaacag gatcataaac agctcacgca gcgacatccc tggcagttcc
     2881 tggctggcaa tctggctttt cgctttaccg aagtaagcgc gatgtaacat cgccagcgaa
     2941 taaacagatg caaagaccag cccaaaggta gagataacgg taatcaccgg gacaacctgg
     3001 aagctgccga acagaatcat aaattcgccg acgaagttac cggtgccagg catcccaagc
     3061 gttgccaccg caaagaacag cgacagtgct ggcagccatt tcatcttgct ccacagaccg
     3121 cccatcatgc gcatgtcgcg ggtatggata cgttcataaa gctgaccgca gagaataaac
     3181 agacccgccg ccgacaagcc gtgcgcaatc atctggatta ccgcgccctg gtaggccaac
     3241 tggctgccgg tgtagattgc aatcagcacg aagcccatgt gggaaaccga ggtgtaggcg
     3301 atcagacgtt tgatatcggt ctgggcgaag gccatccacg caccgtagaa gatgccgata
     3361 acacccagcc acatagcaat tggcgcgaac tctgccgacg cgttcgggaa cagcggcagg
     3421 gagaaacgca gcaaaccgta agcggcagtt ttcagcaaga tccccgcgag gtcaacggaa
     3481 ccggcggtcg gagcctggga gtgcgcatcc ggcagccagc catgcagcgg aaccaccggc
     3541 attttgactg cgaaggcgat gaagaagccc agcattaaca ggtattccac accactggac
     3601 attggcgtat tcagcagctc ttcatagttg aaggtccaga cgccggtcgc attgtagtga
     3661 acaaaaacca gcgccaggat ggcgatcaac atcaccagac cactcgcctg ggtgtaaatg
     3721 aagaacttgg ttgccgccgt gatacgcgtt ttaccgtcag aggctttatg cccccacagt
     3781 gcgatcagga agtacatcgg caccagcatc atttcccaga agaagaagaa caggaacatg
     3841 tcgatggcaa ggaacacgcc gataacgccg cccaggatcc acatcaggtt gaggtggaag
     3901 aagccctgat atttttcgat ctctttccac gaacagagta ccgccagcac accgagcaga
     3961 ccggtcagca cgaccatcag cagcgacagc ccgtcaatgg cgagatggat agagatacca
     4021 aaacgcggga tccacggcat gtcgaattca gactgccact gcggaattcc ggcggattgc
     4081 gtcagtgaat aaccgccctg caaccacagt tgcagcgaca gcgccagcgt caatcccatg
     4141 gtgatcagcg cgatccagcg cggcaccttg acgccaaagc gttcggtctg ccagcacagg
     4201 aagccgccaa taaagggaat taatattagc cagggtagta acatggcgat ctttattcct
     4261 tgtaaaagtc ccgtcaggac cggattttca acgaattctc acgacagaaa attctcttcg
     4321 gactgggggt agtgtcggat gcgccgcgaa ggcagcttat ccgacctacg ttcttatatg
     4381 cccaggggca atcccacaat ccttaactca acgcagtacc atcaacagtg ccagcaccac
     4441 gaccgcaccg atgctcatgg atgccacata ccagcgcaga taaccgttct cgcttaacag
     4501 cagaccttta cctgcaaagc gggaaagaac cgccgggatg ttcatcattg agttcagcgg
     4561 atcgcgtttc agcaaccagg caatacccag gaacggcttg acgaacactt tgtcgtacag
     4621 ccagtcaaag ccccaggcgt tgtaccacca ggtactcagc aggcggcccg gcgcactgtt
     4681 ggcgatggag gtcaccagag tacgtttacc cagccacagc caggctgcca gcagaatgcc
     4741 gaccaccgcg accacgccag aggtaatttc cagggtcaac atgctgccgt gcgccagttc
     4801 cgtcgtttgc ggaagcacgc cctgcagcgg cggtacaatc agtgcgccaa cgaaggtgga
     4861 aaggatcagc agcacaatca gcggcaggct gtgagttacc cctttcacgg cgtgagcgtg
     4921 aatttgttct tttccgtgga agacgatgaa aatcatacgg aaggtgtaga gcgaggtcat
     4981 aaacgcaccg accagacctg ccaccatcag attgatatga ccattcgcca tcgcacccgc
     5041 gaggatctca tccttactga agaagcccgc agtgaccagc ggcagtgccg acagtgctgc
     5101 gccgcccacc aggaagcaga gataaaccag cggaatagat ttacgcagac cgcccatctt
     5161 gaagatgttc tgttcgtgat ggcaggccag aatgacggaa ccggatgcca ggaacagcag
     5221 cgctttaaag aacgcgtgag tcatcaagtg gaaaatcgcc gcatcccatg cctgcacgcc
     5281 aagcgcgagg aacatgtagc caatctggct catggtagag taagcgagaa cacgtttgat
     5341 gtcggtctgt accagcgcgg caaaaccggc cagcagcagc gtaaccgccc cgacaatacc
     5401 caccagatgc agaacttccg gcgtcatcag gaacaggccg tgggtacggg cgatcaggta
     5461 gacacccgcg gttaccatcg ttgcggcgtg gatcagcgcg gagacaggcg tcgggcccgc
     5521 catcgcgtca gcaagccatg tctgcaacgg caactgcgca gatttaccga ccgcaccgcc
     5581 cagcagcatc agcgtcgccc acatcagcat gttattgccg tcagcaaagt gcgctggtgc
     5641 cagttccacc atttcgcgga agttcagggt gcccagttcg ttgtaaagaa tgaacagtgc
     5701 gaaagcgagg aacacgtcac ccacacgggt cacgacgaac gctttcattg ccgctgcgcc
     5761 attcttcgga tcggtgtaat agaacccgat cagcagatag gagcacaggc ccacgccttc
     5821 ccagccgagg tacatcagca gcaggttgtc ggcaagcacc agaaccacca tgctggcgat
     5881 gaacaggttg gtgtaagcga agaagcgaga gtagccctct tcaccgcgca tataccagga
     5941 ggcgtacatg tgaataagga aacccacacc agtgaccacc gagagcatgg tcagcgacag
     6001 gccgtccagc accaggttaa aaccgatgtt aaagtcgcct accgacatcc acgtccacag
     6061 cggctggctg tatgtctgct cgccgttagc gaagaaatca acgccgataa aggcggttac
     6121 cagcgccgcc aggcccacag agcctacgcc gacgatcgcc gagacgtttt cagaccagcg
     6181 cccacgggag aatgccagca ggacgaagcc aatcaatggc aaaataatgg ttaaggcaag
     6241 catgttcatc cgcgcatctc acttactgaa tcgatgttca ggttctggcg acgacggtga
     6301 agttgcagca gcagcgcaag gccgatactc gcttctgccg ccgcgaggct gatggcgaga
     6361 atgtacatca cctgaccgtc ggtctggccc cagtagcttc cggcgaccac gaaggccagc
     6421 gcggaggcgt taatcatgat ttccagacca atcaacataa acagcagatt gcgacggata
     6481 accagaccgg ttaagccaag aacgaataag attgccgcga ggatcagtcc atgttgtaag
     6541 gggatcatgc gtgctcctcc gtttttcttt tcgcgctgtc gtctttacga ttgctcagca
     6601 cttcacccgc acgctcttca cgaccgacgt ggaaggccac aaccagacct gcgagcagca
     6661 gcatagaagc cagttccacc gccagtacgt aaggcccgaa cagcgtaata cccactgctt
     6721 tagcactgat tggcgtaccg tcgataccct gatcgttaac accgaggatg gcgtaaacaa
     6781 tcaccaccag catgatggcc gacaaaattg ccggaccaat ccacacctgc ggtttcagcc
     6841 actggcgttc ctgttcgatt tctgaaccgc ccaggttgag catcatcacc acgaacacga
     6901 acagcaccat aatggcaccc gcgtagacga taatttccag cgcaccggcg aagtaagcgc
     6961 ccagtgagaa gaacaccccg gaaatcgcca gcagcgaaat aatcaggtac agcagtgcgt
     7021 gtaccggatt ggtatgggtg atcactcgca aggtcgcaag tatggctatc aggccacaga
     7081 tataaaaagc gaactccatt gcccctctcc ttacggtaac aggctcttga cgtcgatagg
     7141 cttggcttcg ttctctgctt cgcccttatc tttgccgtcg attgccatac ctgccatccg
     7201 gtagaagtta tattccgggt atttgcccgg accggagatc agcagatcct ctttctcgta
     7261 aaccagatcc tggcgcttgt attcccccat ttcgaaatcc ggggttaact gaatcgccgt
     7321 ggtcggacag gcttcttcgc acagaccaca gaaaatgcag cgtgagaagt tgatgcggaa
     7381 aaattccggg taccagcgac cgtctttggt ttctgctttt tgcagcgaga tacagccgac
     7441 cgggcaggct accgcgcaga ggttacaggc tacgcaacgc tcttcgccgt ccgggtcgcg
     7501 ggtcagaacg atacgaccac gataacgggg cggcagatag accggctctt ccgggtacat
     7561 tcgcgtttcg cgtttggcga acgcgtgcag gccgatcatc cagatactac gaacctgggt
     7621 gccgaaacct actaacaatt cttttaaggt catggtctta ttgcccctta ttgcgcctgc
     7681 cagagaatga cagccgccgt taccagcaag ttgatcagcg tcagcggcag gcagattttc
     7741 cagccgaagg acattacctg gtcataacgc ggacgcggta acgacgcacg aatcaaaatg
     7801 aacatcatca taaagaacgc ggttttcagc gcgaaccaga tgaatggcgg taacaacggg
     7861 ccttgccagc caccgaagaa cagcgtcacc atcaatgcag agatggtcac aatcccgatg
     7921 tactcaccca cgaagaacag accgaacttc ataccggaat attcaatgtg gtaaccatcc
     7981 gccagttcct gctcggcttc cggctggtca aacgggtgac ggtgacatac cgccacgccc
     8041 gcgatggcaa aggtaataaa accaaagaat tgcgggataa cgttccacac atgcgcctgg
     8101 ctgttgacga tgtcggtcat gttgaatgaa ccggcctgcg ccaccacgcc catcaaggaa
     8161 agcccgagga acacttcgta gctcagggtc tgcgcagaag cacgcatcgc acccagcaac
     8221 gagtatttgt tgttacttga ccagcccgca aacagcaccg cgtaaaccgc cagacctgcc
     8281 atcatcagga agaacaaaat cccgatgttc aggtcggcaa ccacccaacc cggactgact
     8341 ggcacaatcg caaaggccag cagcagcgag gtaaaggcaa tcatcggtgc cagggtaaag
     8401 atgacgcgat ccgagaattt cgggatccag tcttctttaa agaacatttt gatcatgtcc
     8461 gcaaccagct ggagcgaacc gccccagcca acacggttag gtccgtaacg gttctggaac
     8521 agacccagca ggcgacgttc gccaaagctc atgaatgccc cgcaggtgac aaccaccagc
     8581 aggatcacca ccgctttgag gatggtcagc aggatctcaa tcagttccgg tgatatccaa
     8641 ctcattgttg tgcctccttg agatcctcaa gatgcgcgcc agccagcacc ggagcaatgc
     8701 cggacatacc catcggcaag cccacctgcc ctgccgtcag tccttcggcg atttcaaccg
     8761 gcagcgtgac cgtgttgcca tcgtaactaa aggagacgcg tgtacctgcg ttcacaccca
     8821 acttcgcggc atccgctggg ttgagtttga tgtacggctg cggcatacgg ctctggaaga
     8881 ccggagcacg ctgtgacaat tcatcgctgc caaacaggtg gtaatacggc gcgatacgcc
     8941 atttcccgtc ctgcggctgg aagcgtgccg gtacgctggt gaagtaatcc agaccatttt
     9001 cgctggtttc aaacagacgc acgcccggat cgccaaagcg cagtttgccg cccacttcgt
     9061 cctggaattt gttccacgcc tgcggggagt tccagcccgg cgcccaggca aacggcactt
     9121 gcgaacggtg cgcagtcggc tggttgttac cttccatcga gaaggtgaac atggtgtcaa
     9181 tatcctgcgg ctgacgcggc tcatgaacgc tgatattggc gcgcatggcg gtacgaccgc
     9241 tgtaacggtg cggttcacgg gccagtttct gcccacgaat acggaatgtc gcatccggcg
     9301 cagcatcttt gatacctgcc agttccggga ttttcgccac aacagcgtca atcacatggt
     9361 cgagctgcgt ccagtccact tcacggctca gcagggtgct gtgcagcgag tgtaaccagc
     9421 gccagctttc cagcatgaca gttttgctgt cgtaataagc aggatcgtaa acctggaaga
     9481 aacgttgggc gcggccttcg ttgttgatca ccgtaccgtc gctttcagca aagctggcag
     9541 cagaaagtac cagatgggcg ttttccataa tcgctgtgcg ttgatgatca accaccatca
     9601 ccagcggtgc tttagccagc gcagcattca cgcggatagc agaagcgtga cgatgcagat
     9661 cgttttccaa caccaccacc gcgtcggcgc gtccggtttc cagttcggtt aacgcttctt
     9721 caagcgaacc gccacccata atgcccagcc ccatgctgtt gacggaacgg gcaatcatgg
     9781 tgataccgac gtcagcgccg cgacctttca gggctttcgc gacgttagcc gccgcctgaa
     9841 tcacctctaa gctaccggcg ttcgtcccgg agataatcaa cggtttcttc gcacctgcca
     9901 gtgcctgcac gatgacgtcg attttgcttt gcagctcagg ttcgataccg tcaaccgctg
     9961 gtgcagagtt atccagcgca tgggcgatgg caaaacctaa acgcgcctga tcttcaaccg
    10021 gtgcgcggta agtccacgcc gcgatatcat ccagacgggt gtcatcaacg ttggtaacaa
    10081 acagcggatg cttcgcacgt tgaccgatgt tgaggattgc cgcaatctgc cagtcagcca
    10141 ctttctgtgc tgccgccatt tcgcgcgctt tacctttcac agcctgacgc actgccagcg
    10201 cgacgcgcgc gccggtctgg gtaacgtctt cgcccagcac cagtaccgca tcgtaagatt
    10261 cgatttcgcg cagagccgga gtataaatgc cgccttcacg cagcactttc agcgccagtt
    10321 gcagacgttc ctgctcaccg tgagcgatac cggtgtagaa gttttcttcg cccaccagtt
    10381 cacgcagcgc aaagttgctt tccacgctgg cacgcggaga accaataccg atcactttct
    10441 tcgactgacg cagaatatct gctgcgccct gcattgcctg ttcggcgttg agggtaatga
    10501 aatcatcgcc acgacgctgt actggctgac gcggacgatc tttcaggttg acgtaaccgt
    10561 aaccgaaacg accacggtcg cagaggaagt agtggtttac cgtaccgttg taacggtttt
    10621 cgatacgacg cagttcgcca taacgctcgc cggggctgat gttacagccg atggaacatt
    10681 gctggcagat gctcggcgca aactgcatat cccatttacg gttgtaacgc tcggagtgcg
    10741 ttttgtcggt aaatacgccg gtcgggcaaa tttcgaccag gttaccggaa aattcgcttt
    10801 ccagcgtgcc gtcttccggg cgaccgaagt agacgttgtc gtgcgcaccg taaacgccca
    10861 gatctgtacc gtcagcgtaa tctttgtagt aacgcacgca gcggtagcag gcgatgcagc
    10921 ggttcatttc gtgagagatg aacggtccca aatcctgatt acggtgggta cgtttggtga
    10981 aacggtagcg acggaagctg tgtccggtca tcacggtcat atcctgaaga tggcagttac
    11041 cgccttcttc acataccgga cagtcgtgcg ggtggttggt cattaaccac tcgaccacac
    11101 tttcacggaa ctgtttcgct tcttcgtcgt caatggaaat aaaggtgcca tcggaagccg
    11161 gtgtcataca ggacatcacc aggcgaccac gcgtgtcttc cgcgttttgg tattgcttca
    11221 ccgcacactg gcggcaagca ccgacgcttc ccagcgccgg atgccagcaa aagtaaggaa
    11281 tatcaaggcc cagagacaga caagcttcca gcaggttgtc cgctccgttg acctcgtatt
    11341 ctttgccgtc tacatgaatt gtagccatta gcatgcttcc agttttctca gtcagagact
    11401 gagcgttaat cgaaattcgg ttaccagcgc tctttcagca ggttcggctg aatcccatta
    11461 atcaaatggg tattgctgaa cggctgtttg attcccgcct caaattcttc gcggaaatat
    11521 ttgatggcgc tctgtaacgg ctccactgca ccaggtgcgt gggcacagaa agttttaccc
    11581 gggcctaaga atcgacacag ttgctcaagt gtttcgatat cgcctggctg accttcacca
    11641 cgctccagcg cacgcagaat tttcacgctc cacggcagac cgtcgcggca cggcgtacac
    11701 cagccgcagg actcacgggc gaaaaactct tccaggttac gcaccagcga caccatgttg
    11761 atctcatggt caaccgccat cgccagcgcc gtacccagac ggcttcccgc tttaccgata
    11821 ctttcgaatt ccatcggcag gtcaaggtgc gcttcggtca ggaagtcggt gcccgcgcca
    11881 cctggctgcc aggctttgaa tttcagaccg tcacgcatac caccggcgta atcttcgagg
    11941 atctcgcgcg cggtggtgcc gaacggcagt tcccacaggc ccggattttt cacccgaccg
    12001 gagaagccca tcagcttggt gccagcatct ttacttttcg agatgttctg ataccactcc
    12061 acgccgttag cgaggatcgc cggaacgtta cacagggttt cgacgttgtt gacacaggtc
    12121 ggtttacccc atgcgccgga ggttgccggg aagggtggct tcgagcgtgg gttagcacga
    12181 cgtccttcca gggagttgat taacgctgtt tcttccccgc agatgtagcg ccctgccccg
    12241 gtatggacga acagttcgaa atcgaaacct gttcccataa tgtttttgcc aagcagaccc
    12301 gcttcggtgg cttcggcaat ggcacggcgc agattaactg ccgcttcgat atattcgcca
    12361 cgcaggaaga tgtagccacg gtaagctttc agcgcaaacg cggagatgag cataccttcc
    12421 accagcaggt gcggcagttg ctccatcaac aggcggtctt tataggtgcc cggctccatt
    12481 tcatcggcat tacacagcag gtaacggatg ttcatggatt cgtctttcgg catcaggctc
    12541 catttcaggc cagtcgagaa gcccgcgccg ccgcgccctt tcagaccagc gtcttttacc
    12601 tgattaacga tttcgtccgg agacagcccg gtcagcgcct tacgcgcgcc ttcgtaaccg
    12661 tttttgctgc ggtattcgtc cagccacact ggctgtttgt catcgcgcag acgccaggtc
    12721 agcggatgcg tttcgggagt acggataatg tttttcattt ataccgctcc agcagttcag
    12781 ggatcgcttc cggggtcaga tgcgcgtgag tgtcctcatc gatcatcatg tttggccctt
    12841 tatcacagtt ccccaggcag caagttggca gcagcgtaaa gcggccatca aatgtcgttt
    12901 gccctggttt gatgttcagc tttttctcga gcgccgcctg aataccctga taaccgttga
    12961 tatgacagac cacgctgtca caataacgga tcacatggcg accaaccggc tggcggaaga
    13021 tctgactgta gaacgttgcc acaccttcga cgtcgcttgc cggaataccc agcacatcgg
    13081 cgatcgcgtg gatcgcacca tccggcaccc agccacgctg cttctgaacg attttcagcg
    13141 cttcaatgga cgccgcacgc gggtcttcgt agtggtgcat ctcgtgctcg atcgcttcac
    13201 gctctgccgc actcagctca aaagcctcgg tttgtggttg ttgattctcg tgcataatta
    13261 gcggtccaca tctgacataa caaaatcgat actgcccaga taaacaatca ggtcagacac
    13321 caggctgccg cggatcgccg ccggaatttg ctgcaaatgc gcaaagctcg gggtacgaac
    13381 acgggtgcgg taactcatgg tgctgccgtc gctggtcagg tagtaactgt tgatcccttt
    13441 ggtcgcctca atcatctgga aagattcatt ggcaggcatc accggacccc acgacacttg
    13501 caggaagtgg gtgatcaggg tttcgatatg ttgcagcgtg cgctctttcg gcggcggcgt
    13561 ggtcagcggg tgatccgctt tgaacgggcc ttccggcatg ttgttgaggc actgctcaag
    13621 aatgcgcaga ctctggcgca gctcttccac tttaagcatt acgcgggtgt agcagtcaga
    13681 aacgccacca cccaccggga tttcaaagtc gaagttttca tagccagaat aaggacgcgc
    13741 cttacgcacg tcgaagtcga tcccggtagc acgcaggccc gcgccagtgg tgccccactc
    13801 cagcgcctct ttcgcgccat aggcggcaac gccctgggaa cgacctttca gaatggtgtt
    13861 ttgcagcgcc gctttctcgt aagacgccag acgtttcggc atccagtcga ggaactcacg
    13921 cagcaggcga tcccagccgc gcggcaggtc gtgcgctacg ccgccaatac ggaaccacgc
    13981 cgggtgcata cggaaaccag tgattgcttc caccagatcg taaattttct gacgatcggt
    14041 aaaggcgaag aacactggcg tcattgcgcc gacgtcctga ataaaggtcg agatatacag
    14101 caggtgactg ttgatgcgga acagttcgga gagcataacg cgaatgacgt taacgcgatc
    14161 cggcacggtg atcccggcca gtttctctac cgccagcacg taaggcattt cgttaacgca
    14221 gccgccgagg tattcgatac ggtcagtata cggaatgtag ctgtgccagg actggcgttc
    14281 gcccattttc tccgcaccac ggtggtggta accgatgtct ggtacgcagt cgacaatctc
    14341 ttcgccatcg agttgcaaaa cgatacggaa agccccgtgc gccgacgggt ggttcggacc
    14401 gaggttgagg aacatgaagt cctcgttttc ggtgccgcgc ttcatccccc actcttccgg
    14461 tttgaaggtc agggcttcca tctccagatc ctgtttggct ttggtcagct caaacggcga
    14521 gaattcggta gcgcgcgccg gataatcttt acgcagcggg tgacctttcc aggtttgcgg
    14581 catcatgatg cgtcgcaggt tcgggtgacc gtcgaaagta atgccaaaca gatcccaggt
    14641 ttcacgctca taccagttag cgttcgggaa cagtttggtg aaggtcggta cgtgcaggtc
    14701 gttttctgcc agcgccacct tcagcatgat gtcgcggtta cgatcgatag aaatcagatg
    14761 gtagaaaacg gaaaaatccg cggcaggtaa cccttcgcgg tgtgtgcgca gacgttcgtc
    14821 catgccgtgt aagtcaaaca gcatgacgta aggtttcggc agtttcttta agaaatcgcc
    14881 aacttccagt aattgttcac gcttgatcca cacaacggga accccggtgc gagtcgcctg
    14941 aacagtaaag gcatccggcc caaaacggtt gcgcagttcg ccaatcaccg gatcatcaag
    15001 atgatcgcgg gtctgccagg cgggttcttg cgcggttaag tcggtcatat tgttcaccat
    15061 tgcaaatggt ccgtggtgac tgtcggcgca gtaagcttcg cgaaatagat ttaggaataa
    15121 gcgaagaaaa atccctttgc cgacaggcgc aaattaaatc tcgtcaggtg tacgcaggtt
    15181 agttacggca atgcgttcac cgcgcttgcg ttcgcgctct gattgcatat tggcgcgata
    15241 aacgccctga tcgccaacca cccaggagag cggacgacgt tctttgccga tagattcctg
    15301 caacagcatc agtgcctgca tgtacgcttc aggacgcggc gggcagcccg ggatatacac
    15361 atcaaccggg atgaatttat cgacgccctg cacaacggaa taaatatcgt acataccacc
    15421 agagttggca caggcaccca ttgagataac ccattttggt tccagcatct ggtcatacag
    15481 acgctgaatg accggtgcca ttttggtaaa gcaggttcct gcaaccacca tcaggtcagc
    15541 ctgacgcggc gaagcacgca atacttctgc gccaaaacgc gccacgtcat gcaccgcggt
    15601 aaacgaagtc accatctcaa cgtaacagca ggaaagaccg aagttatacg gccaaattga
    15661 gtttttacga ccccagttaa ccatgtcatt gagcttgccc ataaacacgt ttttgttaac
    15721 ttcttgctcc agagggtcgg ttacgatctc ctgcttttgc agggggtaac ggtcgttctc
    15781 accgttggga tctatgcggg tgagcgtata atccatctta atgcctcgcg gttagcgttg
    15841 acgattagcg atactgttcg tttccgggtt catacgctcg cggcgtgaac gcgcgggcgt
    15901 ccagtccagc gcgccaatac gcaccagata aaccagacct gccagtaaca caaaaataaa
    15961 aattgcagct tccacaaagc ctacccagcc gctttcgcgg atagaggttg accatgcgaa
    16021 cagatacagc gcttcaacgt cgaagataac gaagaacatg gccaccagat aaaacttggc
    16081 ggacaggcgt aagcgggcgg agccgaccga gtcgataccg gattcaaacg gcacgttttt
    16141 cgacctcgcg cgtgcgcgac cgcctaaaaa ccaaccgcct accagcatca ggcaacacag
    16201 gccaatggca acgataagaa agatagcgaa tgcccagtga tgagcgatga cttcagtgga
    16261 tgttgacata ctcattgctt actcatcaaa agtagcgcca gattcactgc tcttcacggc
    16321 agatggacgc cacatcgatt catggggagg aataaaaaaa accttacaat cactgtagaa
    16381 attcttttat acagctaatt gatgtggtct tttactcctt tctataacct tttgtcaact
    16441 ttaacaaaag tttcttcaca ttagtttaca taatatcaac accattagca tttaatgccc
    16501 tttcacccca gatccttgac gactccagga taattagatg ttgttgaatc gtgtccgttg
    16561 tgaagcaatg gaaaaaatac gggtctattt tgacaggaat ttgtgtcgat tcctcccccc
    16621 aaaagagagt attttcttga tctgtgacac gcttttgtca ttccataaca aaaacgcagc
    16681 aacaaattta cgtatttttt aacatcattg tagcaggtga tttttttcag gcgattattt
    16741 gtgcgttcgg gacgtgaatc tctggtgatt gaaaaatgaa cagttttgtg cgttctgcac
    16801 tatgcgagga aggattttta ctaaaaaaaa gccgctgggg tttaaaacac ccccagcggc
    16861 tcgtttttta cactattgtc tcaggaatta tctatcgtcc gtcgattact cgatatccct
    16921 ttcaatcaac aatgaatcat ccccttccgg agcagacatt gggctgtatt gccacggatt
    16981 atggtagctc tccatcgcct gatagatcac ctgggccagc tcattattac tggacgggtc
    17041 atagcacagc aaatattcgg tatcaggtag cggcggtaag ccatctactc cactcagcac
    17101 acgcaggtcc gggctcatca tttcaaccgg ccttgccgtc acgccaagac cggctttcac
    17161 tgccgcacga acggccggaa gcgtcgaggc gacataagcc agtcgccatg gaatatctgc
    17221 tttattaagc gtcgccagca ccatatcgcg aaacggactt ggatcatcca gcaatacaag
    17281 agggatcggc tcaccttttt gcaatacgta ttccgctgcg cagtaccagt gtgttggcga
    17341 ggtacgcagg ttcaatgcct taaaggtaga aggacgatgg gtggttacca tcaaatccac
    17401 ttcctgggat tccagcattt ctgccatata ggcattacgt ttaacgcgga catccagcgc
    17461 taatttcgga taaaccgaac tcacgcgatt taacaggaaa ggtaagatcg tatcggcaga
    17521 ttcatctgaa gcaccgatag ttaacacgcc ctgaagatta ctgaacatta atgatgagca
    17581 ggcctcatca ttaaaacgca ggattttcct ggcgtaacca agaagttgaa tgccatgttc
    17641 agttaacagt ttgttgcgac cgtgacgagc gaacagttct ttcccaacga gttgttccag
    17701 acgctgcatt tgctgactta cggcggactg agtacgacac acagcggcag ctgcggcagc
    17761 aaaagtgttc agatcggcaa cagcaacaaa tgttctcagc agatcgaggt cgaggttaat
    17821 tatcggacga tttgcactta tcatatatta tcacttactg gcggctcata ctgagctggt
    17881 taatgctgtg cacacacaaa caagcaattc catttgtaat gtgcctccct ggcagtttca
    17941 tccggaaccc ggacgaaagt aaaaatgcat atgagttgca ctaaaaaagc gactcacatt
    18001 gttccgttat aatgcctgaa gtagatcaca gaatatatct tcagggatcg catatctatt
    18061 aagttactca ctcttttcta tttatgacat gcgcgtgttt gtataaatgt aaatgtgagt
    18121 ccttgttcca ctctcgtgca gcatcgctgg tcatacgcga acacgtacaa acagcaatgg
    18181 tgaggcggca tcaagagcag ggatccgtca tttatgcgag cattttaccc caaaaacctt
    18241 ttatttataa gggtcattgc gaattatctg atgc
//
LOCUS       DAPHBJ010000086        18096 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.086, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000086 DAPHBJ010000000
VERSION     DAPHBJ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.086"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..677
                     /locus_tag="RHZ76_002560"
     CDS             <1..677
                     /locus_tag="RHZ76_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV2813178.1"
                     /translation="MQAGLKEVMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAG
                     ESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLV
                     KTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERKVLRLLGKGWGINQIASL
                     LKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELR
                     REMSHS"
     gene            635..1312
                     /gene="bglJ"
                     /locus_tag="RHZ76_002561"
     CDS             635..1312
                     /gene="bglJ"
                     /locus_tag="RHZ76_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HDV2813179.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1350..2138)
                     /gene="fhuF"
                     /locus_tag="RHZ76_002562"
     CDS             complement(1350..2138)
                     /gene="fhuF"
                     /locus_tag="RHZ76_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HDV2813180.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2279..2515
                     /locus_tag="RHZ76_002563"
     CDS             2279..2515
                     /locus_tag="RHZ76_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HDV2813181.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLILTP
                     VMLYHKQLRHYILLPSCLALSAGIMLMLMNLNQG"
     gene            complement(2554..2640)
                     /locus_tag="RHZ76_002564"
     tRNA            complement(2554..2640)
                     /locus_tag="RHZ76_002564"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2604..2606),aa:Leu,seq:cag)
     gene            complement(2949..3980)
                     /gene="rsmC"
                     /locus_tag="RHZ76_002565"
     CDS             complement(2949..3980)
                     /gene="rsmC"
                     /locus_tag="RHZ76_002565"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HDV2813182.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4083..4496
                     /gene="holD"
                     /locus_tag="RHZ76_002566"
     CDS             4083..4496
                     /gene="holD"
                     /locus_tag="RHZ76_002566"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HDV2813183.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4465..4911
                     /gene="rimI"
                     /locus_tag="RHZ76_002567"
     CDS             4465..4911
                     /gene="rimI"
                     /locus_tag="RHZ76_002567"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDV2813184.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            4926..5603
                     /gene="yjjG"
                     /locus_tag="RHZ76_002568"
     CDS             4926..5603
                     /gene="yjjG"
                     /locus_tag="RHZ76_002568"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HDV2813185.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLMISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            5694..7283
                     /gene="prfC"
                     /locus_tag="RHZ76_002569"
     CDS             5694..7283
                     /gene="prfC"
                     /locus_tag="RHZ76_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDV2813186.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            7676..8281
                     /gene="osmY"
                     /locus_tag="RHZ76_002570"
     CDS             7676..8281
                     /gene="osmY"
                     /locus_tag="RHZ76_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HDV2813187.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            8408..8569
                     /locus_tag="RHZ76_002571"
     CDS             8408..8569
                     /locus_tag="RHZ76_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HDV2813188.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            8691..9764
                     /locus_tag="RHZ76_002572"
     CDS             8691..9764
                     /locus_tag="RHZ76_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HDV2813189.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            9761..10543
                     /locus_tag="RHZ76_002573"
     CDS             9761..10543
                     /locus_tag="RHZ76_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV2813190.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(11060..11923)
                     /locus_tag="RHZ76_002574"
     CDS             complement(11060..11923)
                     /locus_tag="RHZ76_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HDV2813191.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNHCGECVPQCPHQALQIVDGKVVWNAAVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VGRLIFPALYL"
     gene            complement(11895..13445)
                     /locus_tag="RHZ76_002575"
     CDS             complement(11895..13445)
                     /locus_tag="RHZ76_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HDV2813192.1"
                     /translation="MPTSDENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPELFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            13703..14482
                     /gene="deoC"
                     /locus_tag="RHZ76_002576"
     CDS             13703..14482
                     /gene="deoC"
                     /locus_tag="RHZ76_002576"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDV2813193.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            14609..15931
                     /gene="deoA"
                     /locus_tag="RHZ76_002577"
     CDS             14609..15931
                     /gene="deoA"
                     /locus_tag="RHZ76_002577"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HDV2813194.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            15983..17206
                     /gene="deoB"
                     /locus_tag="RHZ76_002578"
     CDS             15983..17206
                     /gene="deoB"
                     /locus_tag="RHZ76_002578"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDV2813195.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            17286..18005
                     /gene="deoD"
                     /locus_tag="RHZ76_002579"
     CDS             17286..18005
                     /gene="deoD"
                     /locus_tag="RHZ76_002579"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDV2813196.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         4326 a         4634 c         4879 g         4257 t
ORIGIN      
        1 ttatgcaagc agggttaaaa gaggtcatga ggactcactt ccctgaatat gaaataatat
       61 ccagcgcctc tgcggaggac cttaccttat tacaattacg tcgttccgga ttagtcattg
      121 ctgatttagc cggtgaaagt gaagatccac gttctgtttg tgaacattat tattctttaa
      181 tctcacaata tcgggaaatt cactgggttt tcatggtgtc acgctcctgg tattcccagg
      241 cagtagaact gctcatgtgc cctacggcga cgttattgtc tgatgttgaa cccattgaga
      301 atctggtcaa gaccgtacgt tccggcaata cgcacgcaga gcgtatcagc gccatgctga
      361 cctctccggc aatgactgaa actcatgatt ttagctatcg ctccgtcatt ctcactcttt
      421 cagagcgcaa ggtactgcgg ctattaggta aaggatgggg catcaaccag atagcttcat
      481 tgcttaagaa aagtaataaa actatcagcg cccaaaaaaa cagtgcgatg cgtcgactgg
      541 caattcacag caacgctgaa atgtatgcat ggataaatag cgcgcagggt gcaagagaac
      601 ttaacttgcc ttctgtttat ggagatgccg cagaatggaa cacagccgaa ttaagaagag
      661 aaatgtcgca ctcatagaaa aatgcgtcat gagtagtatc ggtattgaga gtttattcag
      721 aaagtttgcg ggtaaccctt ataagctcca tacctatacc agtcaggagt catttcagga
      781 tgccatgtcg cggatctcgt ttgcggcggt cattttttct ttttctgcca tgagaagtga
      841 gcgcagagag ggattatctt gcctgactga actggcgatt aagtttccgc gtacccggcg
      901 tttagttatt gcggatgatg atattgaagc tcggctgatt ggttcattgt cgccatcacc
      961 gctggacggt gtattaagta aagcgtcaac gctggagatt tttcatcagg aactcttttt
     1021 gtcattaaat ggtgtacgtc aggcgaccga ccgactgaac aatcagtggt acattaacca
     1081 aagccggacg ctaagcccca cggagagaga aatattgcgc tttatgtcgc gtggctactc
     1141 aatgacacaa attgccgagc agcttaaacg caatatcaaa acgatccgtg cacataaatt
     1201 taatgtgatg tcgaaactgg gcgtcagttc tgatgcaggg ttgttggagg ccgcagatat
     1261 tctgttatgt atgcggcatt gcgaagcaag taatgtgttg catccctatt aatccgcatg
     1321 atgccgggtt tacttccccg gcagtgcttt catttcagcg tacaatcgcc acattgctgc
     1381 acatccggta agcgataacg ctggcagcaa gtgcggcgca ccagcaggcc atcgcgcagt
     1441 accacggtac gccagagtgg attatcttca ccgttcgtga gcgttttctc aaaaaagagg
     1501 gcatggcgca gcgattcaac cgtcgcctcg ccgagcagct gcttcatctc agtgagatac
     1561 cagttgatca aataaccggt attactccag ataagtttgc cgttgatctc tccggtcgct
     1621 tctagtgcct gcacaaccgg aaccagcgcc tggctgatta acgtttccat tcgctgctgc
     1681 ggcgaatgtg gtgttgcgtt tttatcttca cacacatcga cccagaaaca ggcgacgcgt
     1741 ccggtttcgt gaaactcagc atggaaatgt tccggcgaca catctaatgc cttttcctgc
     1801 gtcagtagcg ccagcattaa tggtggcacc atcaggccga tataccattg tgcccatagt
     1861 gagatcagcg gtttgttctc gcggatcatc gttggttggt tgcgatagat atgatcggaa
     1921 tagaccgcga gtagagaact tagcgcattc ggtgatgacc attgcgccag cgtcatggcg
     1981 ttaagcggag cgggttcatc caggcggata aactcgagca aatgttcacg atgtttggcg
     2041 atcgtcgcac gtacggcttg tgcaagccca gcatcctgcg gctggagatg cgttcgccag
     2101 atgatatctt catagagcgg tgcggaacga taggccataa tcgggatagt aatctaaatg
     2161 ataatgattg ctaatcctag cgataggttt acccgatagc aagggatttc tctggcttgc
     2221 aaatgataaa aattatcata tgatatcggt tatcattatc aatgaaagag atgaaaccat
     2281 gttgcaacgt acgctgggca gtggctgggg agtgttgctg ccgggattgc tgattgcagg
     2341 gctgatgtat gcggatttat cgccggatca gtggcggatt gtcattctga tgggattgat
     2401 attgacgccg gtgatgctgt atcacaaaca gttacggcat tacattttgc taccatcgtg
     2461 cctggcgctt agtgctggca tcatgctgat gctaatgaat ttgaatcagg gatgaaaaat
     2521 caaggaagaa acaagaaagg aagtaaagat aattggtgcg agggggggga cttgaacccc
     2581 cacgtccgta aggacactaa cacctgaagc tagcgcgtct accaattccg ccaccttcgc
     2641 ataccatcaa ttcttaaaaa gaattgctac cacggaggcg cattctagtg gttttcagct
     2701 tttcgtcaat agttaattat cgccggaggt gtaattgctg gaaaaatgtc catcaggaaa
     2761 ctaacgtgca ggtttggtag ttttgtaggt cggataaggc gttcacgccg catccgacac
     2821 ggtatttggg tgcagatgcc agatgcgacg cgggcgcgtc ttatctggcc tacgaagggc
     2881 taacgtgcag gttttgtagg tcggataagg cgttcacgcc gcatccgaca cggtattcgg
     2941 cgagataatt aacctttctt cgcctggcgg gtcataatgg cgcgatacac cttgaaacgc
     3001 ccggtttgcg caatcacttc atggaagcca aatgtctcat ccagcacgtc cgggtaaggc
     3061 aggaaggcgt tcgctacaat tcgcagctcg ccgccgctat taagatgacg caccgcgccg
     3121 cgaatcagcg tttgcgccgc atccaggctg gtttgcatcc catcgtggaa cggcgggttg
     3181 gagatgatca tatcaaaacg acctttcacc tcggaaaaga cgttgctggc aaagacttca
     3241 ccttcaacac cgttggccgc aagtgttgcg cggctggctt ctaccgctgg cgcagagaca
     3301 tcgcacaagg tgagacgaat tttcggcgaa tggcgcgcaa aggcaactga aagcacaccc
     3361 gcgccacagc cgacatccag cactttacct ttcgtgtgcg gggttaacgt cgagagcagc
     3421 aactggctac cgacatccag accgtcgcgg ctaaacacgc caggcagcgt tttgaccgtc
     3481 aggccatcga cgctgtattc gccccagaat ttatcggcat caaataccgg ctgtttttcc
     3541 agacgaccaa aatagaggcc acagcgacga gcgctgtcga ctttattcaa cggcgcataa
     3601 tctgccagca tctgctcggc gctgcgcacg ccgctgcggt tctcgccaac gacaaaaata
     3661 tctgtcccca ctggcagcag agaaagtaaa ttcatcaact ggaactgggc ttccggtttg
     3721 ttcttcggcc agtagtaaat cagcgtatcg caatctgcga cgtcatccgc cgtggcgacc
     3781 agactgaaac gggcgttatc ccccatctgg cggcttaata cctgccagtg gtggaattgc
     3841 tgggtatgag cacggctggc cgcggtatct aaacgcgcgg gcaggtcatc ctgtaagtct
     3901 ccggcaaaca gaatacggct ttgttcgaaa tcatcactgt gacgcagcaa gacttcactt
     3961 gccggggtaa atgcagacat ggaatgctcc tcaattgata ctggcggcga ttatagccat
     4021 atgttggcgc ggtatcgacg aatttgctat atttgcgccc ctgacaacag gagcgattcg
     4081 ctatgacatc ccgacgagac tggcagttac agcaactggg cattacccag tggtcgctgc
     4141 gtcgccctgg cgcgttgcag ggggagattg ccattgcgat cccggcacac gtccgtctgg
     4201 tgatggtggc aaacgatctt cccgccctga ccgatccttt agtgagcgat gttctgcgcg
     4261 cattaaccgt cagccccgac caggtgctgc aactgacgcc agaaaaaatc gcgatgctgc
     4321 cgcaaggcag tcgctgcaac agctggcggt tgggtactga cgaaccgcta tcactggaag
     4381 gcgctcaggt ggcatcaccg gcgctcaccg aattacgggc aaacccaacg gcacgcgccg
     4441 cgttatggca acagatttgc acatatgaac acgatttctt ccctcgaaac gactgattta
     4501 ccagcggctt accacattga acaacgcgcc cacgcctttc cgtggagtga aaaaaccttt
     4561 gccagcaatc agggcgagcg ttatctcaac tttcagttaa cgcaaaacgg caaaatggcg
     4621 gcgtttgcga ttacgcaagt ggtgctggat gaagctacat tgttcaatat tgcggtcgat
     4681 cctgactatc agcgtcaggg attgggaagg gcgctgctgg aacatctgat cgacgaactg
     4741 gaaaaacgcg gcgtggcgac gctatggctg gaagtccgtg cttcaaacgc tgccgccatt
     4801 gccctgtacg aaagtttagg ctttaacgag gcgacgattc gccgcaatta ctaccccacc
     4861 acggacggtc gcgaagacgc catcatcatg gcgttgccaa tcagtatgta agacaaggtg
     4921 gaataatgaa gtgggactgg attttctttg atgccgatga aacgctgttt acctttgact
     4981 cattcaccgg cctgcagcgg atgtttcttg attacagcgt cacctttacc gctgaagatt
     5041 ttcaggacta tcaggccgtt aacaagccac tgtgggtgga ttatcaaaac ggcgcgatca
     5101 cttcattaca gcttcagcac gggcgttttg agagctgggc cgaacggctg aaagttgaag
     5161 caggcttgct taacgatgcc tttattaatg cgatggcgga aatctgcacg ccgctgccgg
     5221 gcgcggtttc tctgcttaac gccattcgtg gcaacgctaa aatcggcatc atcaccaacg
     5281 gttttagcgc cttgcagcaa gtgcgtctgg aacggacggg cctgcgtgat tacttcgatt
     5341 tgctgatgat ttccgaagaa gttggcgttg ctaaaccgaa taagaaaatt ttcgattatg
     5401 cgctggaaca ggcgggcaat cctgaccgtt cacgcgtgct gatggttggc gacactgccg
     5461 agtccgatat tctcggtggc atcaacgccg ggcttgcgac ctgctggctg aatgcgcaca
     5521 atcgcgagca accagaaggc atcgcgccca cctggaccgt ttcttcgttg cacgaactgg
     5581 agcagctcct gtgtaaacac tgattgcctc ccccccgttg atgggtaaaa tagccgcaat
     5641 ttttcgtttt caacaagcgc ggcgcgatgc cgcttactca agaagaaaga attatgacgt
     5701 tgtctcctta tttgcaagag gtggcgaagc gccgcacttt tgccattatt tctcacccgg
     5761 acgccggtaa aactaccatc accgagaagg tgctgctgtt cggacaggcc attcagaccg
     5821 ctggtacagt aaaaggccgt ggttccaacc agcacgctaa gtcggactgg atggagatgg
     5881 aaaagcagcg tgggatctcc attactacgt ctgtgatgca gtttccgtat cacgattgcc
     5941 tggttaacct gctagacacc ccggggcacg aagacttctc ggaagatacc tatcgtaccc
     6001 tgacagcggt ggactgctgc ctgatggtta tcgacgctgc aaaaggtgtt gaagatcgta
     6061 cccgtaagct gatggaagtt acccgtctgc gcgacacgcc gatcctcacc tttatgaaca
     6121 aacttgatcg tgatatccgc gacccgatgg aactgctcga tgaagttgag aacgagctga
     6181 aaatcggctg cgcaccgatc acctggccga ttggctgcgg caagctgttt aaaggcgttt
     6241 accaccttta taaagacgaa acctatctct atcagagcgg taaaggccac acgattcagg
     6301 aagtccgtat tgttaaaggg ctgaataacc cggatctcga cgctgcggtt ggtgaagatc
     6361 tggcacagca gctacgtgac gaactggaac tggtgaaagg cgcgtctaac gagttcgaca
     6421 aagaattgtt ccttgcgggc gaaatcactc cggtattctt cggtactgcg ctgggtaact
     6481 tcggcgttga tcatatgctg gatggcctgg tggagtgggc ccctgcgccg atgccgcgtc
     6541 agactgatac ccgtaccgta gaggcgagcg aagacaaatt taccggcttc gtatttaaaa
     6601 ttcaggccaa catggacccg aaacaccgcg accgcgtggc gtttatgcgc gtagtatccg
     6661 gtaaatatga aaaaggcatg aagctgcgcc aggtgcgtac tgcgaaagat gtggttatct
     6721 ccgacgcgct gacctttatg gcgggcgacc gttcgcacgt tgaagaagcg tatccgggcg
     6781 atatcctcgg cctgcacaac cacggcacta ttcagatcgg cgacaccttt acccagggtg
     6841 agatgatgaa gttcaccggt attccgaact tcgcaccaga actgttccgt cgtatccgcc
     6901 tgaaagatcc gctgaagcaa aaacagctgc tcaaagggct ggtacagctt tccgaagagg
     6961 gcgcggtgca ggtgttccgt ccgatctcca acaacgattt gatcgttggt gctgttggtg
     7021 tgctgcagtt tgatgtggtg gtatcgcgcc tgaagagcga atacaacgtt gaagcagtat
     7081 atgaatcagt caacgttgcc actgcccgct gggtagaatg tgcggacgcg aagaaattcg
     7141 aagagttcaa gcgtaagaac gaaagccaac tggcgcttga tggcggcgat aacctcgctt
     7201 acatcgctac cagcatggtc aacctgcgcc tggcgcagga acgttatccg gacgttcagt
     7261 tccaccagac ccgcgagcat taattcttgc ctccagggcg cggtagccgc tgcgccctgt
     7321 caatttccct tccttattag ccgcttacgg aatgttctta aaacattcac ttttgcttat
     7381 gttttcgctg atatcccgag cggtttcaaa attgtgatct atatttaaca aagtaatgac
     7441 atttctgacg gcgttaaata ccgttcaatg cgtagatatc agtatctaaa gccgtcgatt
     7501 gtcattctac cgatattaat aactgattca gaggctgtaa tggtcgttat tcatcaccca
     7561 tcgattttgt gatggcgacc attgacttct gtagagggtg aagtctctcc ctattcagca
     7621 atgcaacctc gtgttgccag gctcaaatta cgagcaaaca tacaggaata aatcgatgac
     7681 tatgacaaga ctgaagattt cgaaaactct gctggctgta atgttgacct ctgccgtcgc
     7741 gaccggctct gcctacgcgg aaaacaacgc acagactacc aatgaaagcg cagggcaaaa
     7801 agtcgatagc tctatgaata aagtcggtaa tttcatggat gacagcgcca tcaccgcgaa
     7861 agtgaaggcg gcgctggtgg atcatgacaa catcaagagc accgatatct ctgtcaaaac
     7921 cgatcaaaaa gtcgtgaccc tgagcggttt cgttgaaagc caggctcagg ccgaagaggc
     7981 agtgaaagtg gcgaaaggcg ttgaaggggt gacctctgtc agcgacaaac tgcacgttcg
     8041 cgacgctaaa gaaggctctg tgaagggcta cgcgggtgac accgccacca ccagtgaaat
     8101 caaagccaaa ctgctggcgg acgatatcgt cccttcccgt catgtgaaag ttgaaaccac
     8161 cgacggcgtg gttcagctct ccggtaccgt cgattcccag gcacagagcg accgtgctga
     8221 aagtatcgcc aaagcggtag atggtgtgaa aagcgttaaa aatgatctga aaactaagta
     8281 attcgtcgta attcgtcctc ccgaactttg tcgggaggcg cgatgtgcac cacactaaaa
     8341 atatcgcatt gagtgatttt cacgctcata tcaagcggtg acattaacta tggtaaagga
     8401 gacgcttatg tttcgttggg gcatcatatt tctggttatc gcgttaatcg ccgccgcact
     8461 tgggtttggt ggtctggccg gtaccgctgc aggcgcagct aaaattgtct ttgtcgtcgg
     8521 gattattctg ttcctggtga gtttgttcat gggccgaaaa cgaccctaga tttcagacca
     8581 ttaagaaaat tttatccaaa gccagtccag cggactggct tttgcggttt tagcgaataa
     8641 taaattgcgt tactttgtca ttctttcaca acaaaaacag gaaggcagag gtggggcagc
     8701 gaatacctgt aacgcttggt aatattgcgc cgttgtcgct aaggccgttc cagcctggac
     8761 gaatagctct ggtgtgcgaa ggcggcggac agcgtggaat tttcacggct ggcgtgctgg
     8821 atgagtttat gcgcgcgcag tttaatcctt tcgatcttta tctcggcaca tctgccgggg
     8881 cgcagaacct ctcggcgttt atctgcaatc agcccggtta cgcgcgcaaa gtcatcatgc
     8941 gctataccac aaaacgcgaa tttttcgatc cattgcgctt tgtccgtgga ggaaatctta
     9001 tcgatctcga ctggctggtg gaggccaccg caagccagat gccgttgcaa atggataccg
     9061 ccgcgcggtt gtttgacagc ggcaaatcgt tttatatgtg cgcctgtcgt caggatgact
     9121 acgcgcctaa ttacttttta ccaaccaaac aaaactggct ggatgtgatt cgcgcctcca
     9181 gtgcgatacc tggcttttat cgtagcggag tgtcgctgga aggcattaac tacctggatg
     9241 gcgggatcag tgatgcgatt ccggttaaag aggcggcaag gcagggcgct aaaacgttgg
     9301 tcgtcatccg cactgtgccg tcacaaatgt actacacgcc gcagtggttc aaacgcatgg
     9361 aacgctggct gggtgacagt agcctgcagc cgctggtcaa tctggtgcag catcatgaaa
     9421 ccagctatcg tgacattcag caatttattg agaaaccacc gggcaagctg cggatattcg
     9481 aaatttatcc accgaaacca ttacatagta tcgcgcttgg cagtcggatt ccggcgctgc
     9541 gtgaagacta taaacttggg cgtttatgcg gtcgttattt cctcgccacg gttggcaagc
     9601 tattaactga aaaagcgccg cttacccgcc atctggtgcc agtggtgacg ccggaatcga
     9661 tcgttattcc gcctgcgcca gtcgccaacg atacgctggt tgccgaagtg agcgatgctc
     9721 cgcaggcgaa cgacccgaca tttaacaatg aggatctggc ttgatttgcc gttttatcga
     9781 tacccactgc cattttgatt tcccgccgtt tagtggcgat gaagaggcca gcctgcaacg
     9841 cgcggcacaa gcgggcgtag gcaagatcat tgttccggcg acagaggcgg aaaattttgc
     9901 ccgcgtgcag gcgttagcgg aaaaatatca accgctgtat gccgcattgg ggttgcatcc
     9961 ggggatgttg gaaaaacata gcgatgtgtc tcttgatcag ctacagcagg cgctggaaag
    10021 gcgtccggcg aaggtggtgg cggtggggga gatcggtctg gatctcttcg gcgacgatcc
    10081 gcaatttgag aggcagcagt ggttactcga cgaacaactg aaactggcga aacgctacga
    10141 tctgccggtg atccttcatt cccggcgtac acacgacaaa ctggcgatgc atcttaaacg
    10201 tcacgattta ccgtgcactg gcgtggttca cggtttttcc ggcagcctgc aacaggccga
    10261 gcggtttgta cagctgggct acaaaatcgg cgtaggcggt actatcacct atccacgcgc
    10321 cagtaaaacc cgcgatgtca tcgcaaaatt accgctggca tcgttgttgc tggaaaccga
    10381 cgcgccggat atgccgctca acggctttca ggggcagcct aaccgaccgg aacaggctgt
    10441 gcgggtgttt gacgtgcttt gcgagttgcg cccggaacca gaagatgaga ttgccgaagt
    10501 gttgcttaat aatacgtatg cgttgtttag cgttagtggg tagtttaccc ttcgtaggca
    10561 gcatccggca gtcggcgcat aatgcctgat gcgacgcttg tcgcgtctta tcatgcctac
    10621 aggtttgtgc cgaaccgtag gccggataag gcgttcacgc cgcatccggc agttggcgca
    10681 caatgcctga tgcgacgctt gacgcgtctt atcatgccta caagtctgtg ccgaaccgta
    10741 ggccggataa ggcgtttacg ccgcatccgg cagttggcgc acaatgcctg atgcgacgct
    10801 tgtcgcgtct tatcatgcct acaagtctgt gccgaaccgt aggccggata aggcgttcac
    10861 gccgcatccg gcagtcggcg cataatgcct gatgcgacgc ttgtcgcgtc ttatcaggcc
    10921 tacaggtttg tgccgaaccg taggccggat aaggcgttca cgccgcatcc ggcagttggc
    10981 gcacaatgcc tgatgcgacg ctggacgcgt cttatcatgc ctacaagtct gtgccgaacc
    11041 gtaggccgca tccggcatgt cacaaataca gcgccggaaa tatcaaccgg cccaccccgc
    11101 gcacctttaa tgcatcagcc aacggctcaa cgtcttccgg cgtggcgctc gcccagcttt
    11161 gcgcctcgcc atacacgccg tgggcatgaa acgcgttcag gcgtaccgga acatcgccaa
    11221 gtcccttgat aaacgccgcc agttcttcga tgtgttgcaa ataatccacc tggccaggga
    11281 ttaccagcaa acgcagttcc gccagcttgc cgcgctctgc cagcaaacag atgctgcgct
    11341 taatctgctg attatcgcgt ccggttaatt gttgatgaca ttcgctaccc catgctttga
    11401 gatcgagcat tgcgccgtca cacaccggga gtaatttttc ccagccggtt tcgctcaaca
    11461 tgccgttact gtccaccaga caggtgagat ggcgcagttg cggatcgttt ttgatagcag
    11521 taaacagcgc cactacaaac ggcagttggg tcgtggcttc gccgccgctt accgttatcc
    11581 cttcaataaa cagcaccgct ttgcggacat ggctaagcac ttcgtccacg ctcatggatt
    11641 gcgccatcgg cgtggcatgt tgcggacacc tcttcaggca ggtatcacac tgctcgcaga
    11701 ccgcagcgtt ccacaccact ttgccgtcaa caatctgcaa cgcctgatgc ggacactgtg
    11761 gcacgcactc tccgcaatga ttacagcgcc ccatcgtcca cggattgtga cagtttttgc
    11821 agcgcagatt gcagccctgc aaaaacagag ccagacgact gcctggcccg tcaacgcagg
    11881 agaaggggat aatcttactg actaaagcgc atctgctgtt catggcttat cacgcgcggc
    11941 tggcgttcca gaatacgagt gttgcgtgcg gcttcttcgc ctagccaggt ggtgttggtg
    12001 cgtgaacctt cggcgcgata tttttctaaa tccgacaaac gcaccatata accagtaacg
    12061 cgaaccagat cgttaccgct gacattggcg gtaaattcac gcattccggc tttaaaggca
    12121 ccgaggcaaa gctgtaccag cgcctgcgga ttacgtttga tggtttcgtc gagcgtcaga
    12181 atgtcgctga tgccggaata ataataagca tgatgcgggg cgacagtttg cagatgggtg
    12241 atcggatctg gctcatcgcc atagggtaaa cgcgcgcccg gcgtggtgcc gatatcggaa
    12301 ctgatccccg actgtgcgtg taacatggcg cgtttttgcc agccatattt cacgggggta
    12361 ttggcgacaa actccgccag ttgcgcgctg atgcgataac ccacttcatt tgcggcggct
    12421 tctttaccgt agcgcgcggc aatcccttct ttctcacaca gcaagttaac cgcttccgcc
    12481 agcccataca tgccaaacat tggcacaaaa agttcagggt taatcagccc ttctttcacc
    12541 aggaagctat tctcaaagaa gtgtgattgt tgatagagga attcacaccg cgcatcgatg
    12601 atggcgatct gctgctggca gtagtgcggt agagtgcgcg taaagaagtc atccagcgat
    12661 tcgctgcgct cggcaatggc tttcaggtta aggcgtacca gcgtgctgcc accacccgcc
    12721 agcggcagtg agttgtaaca gctcacaatc ccgtagcccc cttttgtgaa aattttatca
    12781 tgcaccggac cgttggcgat gtgcggtttg ctacattcac agatgttctt cgccacttcc
    12841 agcagtaggt catcaggggt gatttcagga tcgtagataa aggtcaggtt cggtgaaacc
    12901 tgcttcaact ctgcgtctgc acgtaagatt gcacgggtaa tgggcgaatc agacgggccg
    12961 atattggcgt gcataaaggc gtctggcagg gttctgtcga ggtaacgcca gaaacgtttt
    13021 attcgaatat cgatctcgtc ttgtgttaga attctaacat acggttgcaa caacgcatcc
    13081 agttgcccca ggtagaccgg catcgatgtg accgacggta cgtggtggta aagaatggtc
    13141 agcagagaga gtgcgtcatc aagatctttc gcaccttcca gctccagcca ttcggaaccg
    13201 ttcgccagaa aacgggcgta atcgggtaag acatagcgcg gtttgtacgg cgcatgacct
    13261 tcaaacatat cgcagattac accctcatcc agcgcacggc gggcttcggc aggcagctgc
    13321 ggataaggca gattgttttc tgcttcaagt gccagaaaat ggcgcttctg ctccgggcta
    13381 agcactgggc tggtgacaat ttgctggcaa cgttgttgca gtgcattttc atcagaagtg
    13441 ggcatcttct tttcctttta tgccgaaggt gatgcgccat tgtaagaagt ttcgtgatgt
    13501 tcactttgat cctgatgcgt ttgctaccac tgacgcattc atttgaaagt gaattatttg
    13561 aaccagatcg cattacagtg atgcaaactt gtaagtagat ttccttaatt gtgatgtata
    13621 tcgaagtgtg ttgcggagta gatgttagaa tactaacaaa ctcgcaaggt gaattttatt
    13681 ggcgacaagc caggagaatg aaatgactga tctgaaagca agcagcctgc gtgcactgaa
    13741 attgatggac ctgaccaccc tgaatgacga cgacaccgac gagaaagtaa ttgctctgtg
    13801 tcatcaggcc aaaaccccgg tcggcaatac cgccgctatc tgtatctatc ctcgctttat
    13861 cccgattgct cgcaaaacac tgaaagagca gggcaccccg gaaatccgta tcgctacggt
    13921 aaccaacttc ccacatggta acgacgacat cgaaatcgcg ctggcagaaa cccgtgcggc
    13981 aatcgcctac ggtgccgatg aagttgacgt ggtgttcccg taccgcgcgc tgatggcggg
    14041 caacgagcag gttggttttg accttgtgaa agcctgtaaa gaggcttgcg cggcagcgaa
    14101 tgtactgctg aaagtgatca tcgaaaccgg cgaactgaaa gacgaagcgc tgatccgtaa
    14161 agcgtctgaa atctccatca aagcgggtgc ggacttcatc aaaacctcta ccggtaaagt
    14221 ggctgtgaac gcgacgccgg aaagcgcgcg catcatgatg gaagtgatcc gtgatatggg
    14281 cgtagaaaaa accgttggtt tcaaaccggc gggcggcgtg cgtactgcgg aagatgcgca
    14341 gaaatatctc gccatcgcag atgaactttt cggtgctgac tgggctgatg cgcgtcacta
    14401 ccgctttggt gcttccagcc tgctggcaag cctgctgaaa gcgctgggcc acggtgacgg
    14461 taagagcgcc agcagctact aagtaagatg ctttacgcct gatgcgctgc gcttatcagg
    14521 cctacgagac gtatctaccc gtaggccgga taaggcgtag acgcatccgg caaaagccgc
    14581 ctcatactct tttcctcggg aggttacctt gtttctcgca caagaaatta ttcgtaaaaa
    14641 acgtgatggt catgcgctga gcgatgaaga aattcgtttc tttatcaacg gcattcgcga
    14701 caacactatc tccgaagggc agattgccgc cctcgcgatg accattttct tccacgatat
    14761 gacaatgcct gagcgtgtct cgctgaccat ggcgatgcga gattcaggaa ccgttctcga
    14821 ctggaaaagc ctgcatctga atggcccgat tgttgataaa cactccaccg gcggcgtcgg
    14881 cgatgtgact tcgctgatgt tggggccgat ggtcgcagcc tgcggcggct atattccgat
    14941 gatctccggt cgcggcctcg gtcatactgg cggtacgctc gacaaactgg aatccatccc
    15001 tggcttcgac attttcccgg atgacaaccg tttccgcgaa attattaaag acgtcggcgt
    15061 ggcgattatc ggtcagacca gctcactggc tccggcggat aaacgtttct acgcgacccg
    15121 tgatattacc gcaaccgtgg actccatccc gctgatcact gcctcgatcc tggcgaagaa
    15181 actggcggaa ggtctggacg cgctggtgat ggacgtgaaa gtgggtagcg gcgcgtttat
    15241 gccgacctac gaactctctg aagcccttgc cgaagcgatt gttggcgtgg ctaacggcgc
    15301 tggcgtgcgc accaccgcgc tgctcaccga tatgaatcag gtactggcct ccagtgcagg
    15361 taacgcggtt gaagttcgtg aagcggtgca gttcctgacg ggtgaatatc gtaacccgcg
    15421 tctgtttgat gtcacgatgg cgctgtgcgt ggagatgctg atctccggca aactggcgaa
    15481 agatgacgcc gaagcgcgcg cgaaattgca ggcggtgctg gacaacggta aagcggcaga
    15541 agtctttggt cgtatggtag cggcacaaaa aggcccaacc gacttcgttg agaactacgc
    15601 gaagtatctg ccgacagcga tgctgacgaa agcagtctat gctgataccg aaggttttgt
    15661 cagtgaaatg gatacccgcg cgctggggat ggcagtggtt gcaatgggcg gcggacgccg
    15721 tcaggcatct gacaccatcg attacagcgt cggctttact gatatggcac gtctgggcga
    15781 ccaggtagac ggtcagcgtc cgctggcggt tatccacgcg aaagacgaaa acagctggca
    15841 ggaagcggcg aaagcggtga aagcggcaat taaacttgcc gataaagcac cggaaagcac
    15901 accaactgtc tatcgccgta tcagcgaata acggtatact gatctgatca tttaaatttg
    15961 aagcactgag tacggagaac atatgaaacg tgcatttatt atggtgctgg actcattcgg
    16021 catcggcgct acagaagatg cagaacgctt tggtgacgtc ggggctgaca ccctgggtca
    16081 tatcgcagaa gcttgtgcca aaggcgaagc tgataacggt cgtaaaggtc cgctcaatct
    16141 gccaaatctg acccgtctgg ggctggcgaa agcacacgaa ggttctaccg gtttcattcc
    16201 ggcgggaatg gacggcaacg ctgaagttat cggcgcgtac gcatgggcgc acgaaatgtc
    16261 atccggtaaa gataccccgt ctggtcactg ggaaatcgcc ggtgtcccgg ttctgtttga
    16321 gtggggatat ttctccgatc acgaaaacag cttcccgcaa gagctgctgg ataaactggt
    16381 tgaacgcgct aatctgccgg gttacctcgg taactgccac tcttccggta cggtcattct
    16441 ggatcagctg ggcgaagagc acatgaaaac cggcaagccg attttctata cctccgctga
    16501 ctctgtgttc cagattgcct gccacgaaga aacctttggc ctggataaac tctacgaact
    16561 gtgcgaaatc gcccgtgaag agctgaccaa cggcggctac aacatcggtc gtgttatcgc
    16621 tcgtccgttt atcggtgaca aagccggtaa cttccagcgt accggtaacc gtcacgacct
    16681 ggctgttgag ccgccagcac cgaccgtgct gcagaaactg gttgatgaaa aacacggtca
    16741 ggtggtttct gtcggtaaaa ttgcagacat ctacgccaac tgcggtatca ccaagaaagt
    16801 gaaagcaact ggcctggacg cgctgtttga cgccaccatc aaagagatga aagaagcggg
    16861 tgataacacc atcgtcttca ccaacttcgt tgacttcgac tcttcctggg gccaccgtcg
    16921 cgacgtcgcc ggttatgctg cgggtctgga gctgttcgac cgccgtctgc cggagctgat
    16981 gtctctgctg cgcgatgacg acatcctgat cctcactgct gaccacggtt gtgatccgac
    17041 ctggaccggt actgaccaca cgcgtgaaca cattccggta ctggtttacg gcccgaaagt
    17101 aaaaccgggc tcactgggtc accgtgaaac cttcgcggat atcggccaga ctctggcaaa
    17161 atattttggt acttctgata tggaatatgg caaagccatg ttctgataat gggtgcggct
    17221 tgatgccctc accccggccc tctccctcag ggagagggtg aaaacatgtt gataaggata
    17281 aaacaatggc taccccacac attaatgcag aaatgggcga tttcgctgac gtagttttga
    17341 tgccaggcga cccgctgcgt gcgaagtata ttgctgaaac tttccttgaa gatgcccgtg
    17401 aagtgaacaa cgttcgcggt atgctgggct tcaccggtac ttacaaaggc cgcaaaattt
    17461 ccgtaatggg tcacggtatg ggtatcccgt cctgctccat ctacaccaaa gaactgatca
    17521 ccgatttcgg cgtgaagaaa attatccgcg tgggttcctg tggcgcagtt ctgccgcacg
    17581 taaaactgcg cgacgtcgtt atcggtatgg gtgcctgcac cgattccaaa gttaaccgca
    17641 tccgttttaa agatcatgac tttgccgcta tcgctgactt tgacatggtg cgtaacgcgg
    17701 tagacgcggc taaagcactg ggcattgatg ctcgcgttgg taacctgttc tccgctgacc
    17761 tgttctactc tccggacggc gaaatgttcg acgtgatgga aaaatacggc atcctcggcg
    17821 tggaaatgga agcggctggt atctacggcg tcgctgcaga atttggcgcg aaagccctga
    17881 ccatctgtac cgtgtctgac cacatccgca ctcacgagca gaccactgcc gctgagcgtc
    17941 agaccacctt caacgacatg atcaaaatcg cactggaatc cgttctgctg ggcgataaag
    18001 agtaattgtg tttcgctgaa aggcgattgt cgtgtgaagc cggagtggga gactgctccg
    18061 gctttttagt atctattcat caatagcaac tgattt
//
LOCUS       DAPHBJ010000087        18005 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.088, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000087 DAPHBJ010000000
VERSION     DAPHBJ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.088"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1415
                     /gene="tnaA"
                     /locus_tag="RHZ76_002580"
     CDS             <1..1415
                     /gene="tnaA"
                     /locus_tag="RHZ76_002580"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HDV2813197.1"
                     /translation="ENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSED
                     VFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQGR
                     GAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFDT
                     GVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAKK
                     YDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPMG
                     GLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYRI
                     AQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIRA
                     VEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIKG
                     LTFTYEPKVLRHFTAKLKEV"
     gene            1506..2753
                     /gene="tnaB"
                     /locus_tag="RHZ76_002581"
     CDS             1506..2753
                     /gene="tnaB"
                     /locus_tag="RHZ76_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HDV2813198.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLVLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWLSSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSSTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            2886..4061
                     /gene="mdtL"
                     /locus_tag="RHZ76_002582"
     CDS             2886..4061
                     /gene="mdtL"
                     /locus_tag="RHZ76_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HDV2813199.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4036..4995
                     /gene="yidZ"
                     /locus_tag="RHZ76_002583"
     CDS             4036..4995
                     /gene="yidZ"
                     /locus_tag="RHZ76_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HDV2813200.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5152..5901
                     /locus_tag="RHZ76_002584"
     CDS             5152..5901
                     /locus_tag="RHZ76_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDV2813201.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            5923..6489
                     /gene="yieF"
                     /locus_tag="RHZ76_002585"
                     /gene_synonym="chrR"
     CDS             5923..6489
                     /gene="yieF"
                     /locus_tag="RHZ76_002585"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HDV2813202.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMAVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6543..7880)
                     /gene="adeP"
                     /locus_tag="RHZ76_002586"
     CDS             complement(6543..7880)
                     /gene="adeP"
                     /locus_tag="RHZ76_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HDV2813203.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8047..8712
                     /gene="yieH"
                     /locus_tag="RHZ76_002587"
     CDS             8047..8712
                     /gene="yieH"
                     /locus_tag="RHZ76_002587"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HDV2813204.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            8779..9252
                     /locus_tag="RHZ76_002588"
     CDS             8779..9252
                     /locus_tag="RHZ76_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HDV2813205.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            9301..9888
                     /locus_tag="RHZ76_002589"
     CDS             9301..9888
                     /locus_tag="RHZ76_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HDV2813206.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(9950..10672)
                     /locus_tag="RHZ76_002590"
     CDS             complement(9950..10672)
                     /locus_tag="RHZ76_002590"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDV2813207.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSTTRRVNLAITAGSSPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(10674..11828)
                     /locus_tag="RHZ76_002591"
     CDS             complement(10674..11828)
                     /locus_tag="RHZ76_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDV2813208.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPVLKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLPMQPSYRNYLNKIRH"
     gene            complement(11855..13471)
                     /gene="bglH"
                     /locus_tag="RHZ76_002592"
     CDS             complement(11855..13471)
                     /gene="bglH"
                     /locus_tag="RHZ76_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HDV2813209.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENYMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13540..14952)
                     /gene="bglB"
                     /locus_tag="RHZ76_002593"
     CDS             complement(13540..14952)
                     /gene="bglB"
                     /locus_tag="RHZ76_002593"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HDV2813210.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKKITIKAP"
     gene            complement(14971..16848)
                     /gene="bglF"
                     /locus_tag="RHZ76_002594"
     CDS             complement(14971..16848)
                     /gene="bglF"
                     /locus_tag="RHZ76_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HDV2813211.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLDEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTSLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(16982..17818)
                     /gene="bglG"
                     /locus_tag="RHZ76_002595"
     CDS             complement(16982..17818)
                     /gene="bglG"
                     /locus_tag="RHZ76_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HDV2813212.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         4535 a         4498 c         4266 g         4706 t
ORIGIN      
        1 tggaaaactt taaacatctc cctgaaccgt tccgcattcg tgttattgag ccagtaaaac
       61 gtaccactcg cgcttatcgt gaagaggcaa ttattaaatc cggtatgaac ccgttcctgc
      121 tggatagcga agatgtgttt atcgatttac tgaccgacag cggcaccggg gcggtgacgc
      181 agagcatgca ggctgcgatg atgcgcggcg acgaagccta cagcggcagt cgtagctact
      241 atgcgttagc cgagtcagtg aaaaatatct ttggttatca atacaccatt ccgactcacc
      301 agggccgtgg cgcagagcaa atctatattc cggtactgat taaaaaacgc gagcaggaaa
      361 aaggcctgga tcgcagcaaa atggtggcgt tctctaacta tttctttgat accacgcagg
      421 gccatagcca gatcaacggc tgtaccgtgc gtaacgtcta tatcaaagaa gccttcgata
      481 cgggcgtgcg ttacgacttt aaaggcaact ttgaccttga gggattagaa cgcggtattg
      541 aagaagttgg tccgaataac gtgccgtata tcgttgcaac catcaccagt aactctgcag
      601 gtggtcagcc ggtttcactg gcaaacttaa aagcgatgta cagcatcgcg aagaaatacg
      661 atattccggt ggtaatggac tccgcgcgct ttgctgaaaa cgcctatttc atcaagcagc
      721 gtgaagcaga atacaaagac tggaccatcg agcagatcac ccgcgaaacc tacaaatatg
      781 ccgatatgct ggcgatgtcc gccaagaaag atgcgatggt gccgatgggc ggcctgctgt
      841 gcatgaaaga cgacagcttc tttgatgtgt acaccgagtg cagaaccctt tgcgtggtgc
      901 aggaaggctt cccgacatat ggcggcctgg aaggcggcgc gatggagcgt ctggcggtag
      961 gtctgtatga cggcatgaat ctcgactggc tggcttatcg tatcgcgcag gtacagtatc
     1021 tggtcgatgg tctggaagag attggcgttg tctgccagca ggcgggcggt cacgcggcat
     1081 tcgttgatgc cggtaaactg ttgccgcata tcccggcaga ccagttcccg gcacaggcgc
     1141 tggcctgcga gctgtataaa gtcgccggta tccgtgcggt agaaattggc tctttcctgt
     1201 taggccgcga tccgaaaacc ggtaaacaac tgccatgccc ggctgaactg ctgcgtttaa
     1261 ccattccgcg cgcaacatat actcaaacac atatggactt cattattgaa gcctttaaac
     1321 atgtgaaaga gaacgcggcg aatattaaag gattaacctt tacgtacgaa ccgaaagtat
     1381 tgcgtcactt caccgcaaaa cttaaagaag tttaattaat actacagagt ggctataagg
     1441 atgttagcca ctctcttacc ctacatcctc aataacaaaa atagccttcc tctaaaggtg
     1501 gcatcatgac tgatcaagct gaaaaaaagc actctgcatt ttggggtgtt atggttatag
     1561 caggtacagt aattggtgga ggtatgtttg ctttacctgt tgatcttgcc ggtgcctggt
     1621 ttttctgggg tgcctttatc cttatcattg cctggttttc aatgcttcat tccgggttag
     1681 tgttattaga agcaaattta aattatcccg tcggctccag ttttaacacc atcaccaaag
     1741 atttaatcgg taacacctgg aacattatca gcggtattac cgttgccttc gttctctata
     1801 tcctcactta tgcctatatc tctgctaatg gtgcgatcat tagtgaaacg atatcaatga
     1861 atttgggtta tcacgctaat ccacgtattg tcgggatctg cacagccatt ttcgttgcca
     1921 gcgtattgtg gttaagttcg ttagccgcca gtcgtattac ctcattgttc ctcgggctga
     1981 agattatctc ctttgtgatc gtgtttggtt cttttttctt ccaggtcgat tactccattc
     2041 tgcgcgacgc caccagctcc actgcgggaa cgtcttactt cccgtatatc tttatggctt
     2101 tgccggtgtg tctggcgtca tttggtttcc acggcaatat tcccagcctg attatttgct
     2161 atggaaaacg caaagataag ttaatcaaaa gcgtggtatt tggttcgctg ctggcgctgg
     2221 tgatttatct cttctggctc tattgcacca tggggaatat tccgcgagaa agctttaagg
     2281 cgattatctc ctcaggcggc aacgttgatt cgctggtgaa atcgttcctc ggcaccaaac
     2341 agcacggcat tatcgagttt tgcctgctgg tgttctctaa cttagctgtt gccagttcgt
     2401 tctttggtgt cacgctgggg ttgttcgatt atctggcgga cctgtttaag attgataact
     2461 cccacggcgg gcgtttcaaa accgtgctgt taaccttcct gccacctgcg ttgttgtatc
     2521 tgatcttccc gaacggcttt atttacggga tcggcggtgc cgggctgtgc gccaccatct
     2581 gggcggtcat tattcccgca gtgcttgcaa tcaaagctcg caagaagttt cccaatcaga
     2641 tgttcacggt ctggggcggc aatcttattc cggcgattgt cattctcttt ggtataaccg
     2701 taattttatg ctggttcggt aacgtcttta acgtgttacc taaatttggc tgatcccttc
     2761 aagaagccag ccatttcgct ggcttcttgc ctgtcaggaa atctcttttg tccaaatggc
     2821 aactcgcctg attctccttc accacgtatg ctttgcgtca ccttactatc aggacgcttt
     2881 agcccatgtc ccgcttttta atttgtagtt ttgccctggt tttactttat cccgccggga
     2941 ttgatatgta cctcgttggt ttaccgcgca tcgccgccga tctcaatgcc agcgaagcgc
     3001 agttgcatat tgcgttctcc gtatatctgg cggggatggc agctgcgatg ttatttgccg
     3061 gtaaagtggc cgatcgttca gggagaaagc cggtcgccat acccggcgcg gcgctattta
     3121 ttattgcctc ggtgttctgt tcactggctg aaaccagcac gttatttctt gcaggccgat
     3181 ttctacaggg gttgggcgca ggctgttgtt acgtagtggc gttcgccatt ttgcgcgaca
     3241 cgctggatga tcgacgtcgg gctaaagtgc tgtcattact caacggtatt acctgcatca
     3301 ttccggtgtt agcgccagtg ctcggacatc tgattatgct taaattcccg tggcagagtc
     3361 tgttctgggc gatggcaatg atgggcatcg cggtactgat gttgtctttg tttattttaa
     3421 aagaaacgcg cccagcgtcc cccgctgctt cggacaaacc acgagaaaat agcgagtcgc
     3481 tgcttaatcg gtttttcctc agccgtgttg ttatcaccac cctcagcgtt tcggtgatcc
     3541 tcactttcgt caatacatcg ccggtattgc tgatggaaat catgggtttt gagcgcggag
     3601 aatacgccac cattatggcg ttgaccgctg gcgtcagcat gaccgtttca ttctccacgc
     3661 catttgcgct gggaattttt aagccacgta cgttgatgat cacctcgcag gtgttattcc
     3721 ttgcagcggg gatcaccctt gccgtttcac cttcccatgc ggtttctctg tttggtatca
     3781 cgctgatttg cgccggtttc tcggtaggtt ttggcgtagc gatgagtcag gcgttaggac
     3841 cattttcatt acgcgcgggc gtagccagct cgaccttagg tattgcgcag gtttgcggtt
     3901 cgtcactgtg gatttggctg gcagcggtgg ttggtatcgg cgcatggaat atgctgatcg
     3961 ggattctgat tgcctgtagc atagtgagcc tgttgctgat tatgttcgtc gcgcctggac
     4021 gccccgttgc cgctcatgaa gaaatccatc accacgcttg atctcaatct gctgctctgt
     4081 ctgcaactgc tgatgcagga gcgcagcgtg accaaagcgg cgaagcggat gaacgtgacc
     4141 ccttcagcgg tgagtaagtc gctggcaaag ttaagagcgt ggtttgacga cccgctcttt
     4201 gtgaactcac cgctgggcct gtcgcccaca ccgctaatgg tcagcatgga gcaaaatctg
     4261 gcggaatgga tgcaaatgag caatctgctg ctggataaac cgcaccacca gacaccgcgc
     4321 ggcctgaagt ttgagttggc ggcggaatcg ccgctgatga tgatcatgct taatgcgctg
     4381 tcgaaacgga tctaccaacg ttacccgcag gcgaccatca aactacgtaa ctgggattac
     4441 gattccttag atgccattac tcgtggtgaa gtggatattg gtttttccgg tcgcgaaagc
     4501 catcctcgct cgcgggagct gttaagctcg ctaccgttag ccattgatta tgaagtgctg
     4561 tttagtgatg taccctgcgt ctggttacgc aaagatcatc cggcactgca tgaaacgtgg
     4621 aatctggaca cctttttacg ttatccgcat atcagcattt gctgggaaca gagcgacacc
     4681 tgggcgctgg acaatgtgtt acaggagctg gggcgcgaac gcacgattgc tatgagcctg
     4741 ccggaatttg agcagtcact gtttatggca gcgcaacccg acaatctgct actggcgacc
     4801 gcgccgcgct actgtcagta ctacaatcaa ctccatcaac tgccgttggt tgctcttcct
     4861 ctcccgtttg acgaaagcca gcaaaaaaag ctggaagttc cttttaccct gctgtggcat
     4921 aaacggaaca gccataatcc gaagatcgtc tggttacggg aaaccattaa aaacctttac
     4981 gcgtcgatgg cataaccgaa tcgtatgaaa cgcgacccaa tttcacaata aaatgtaaaa
     5041 aagttgtaat aagcttgtct gaatcgaact tttagccgct ttagtctgtc catcattcca
     5101 gtaaatgatt actcttatat tcataatgga ccattaagca tggagcgaaa aatggcgact
     5161 cactttgccc gagggatttt aacggaagga catctgattt ctgttcgtct cccctcccag
     5221 tgtcatcaag aagcccgaaa cattccccct catcgtcaaa gccgttttct ggcgtccaga
     5281 ggtttactcg cagaactgat gttcatgctg tatggcattg gcgaattgcc ggaaatcgtc
     5341 accctgccga aaggtaaacc ggttttcagt gataaaaatt tgccttcgtt ttctatttcc
     5401 tatgccggga atatggttgg cgtggcgtta acaaccgaag gtgaatgtgg cctcgatatg
     5461 gaactacagc gtgcgacgcg cgggtttcat agcccacacg cgcccgataa ccacaccttt
     5521 tccagcaatg aatcgctatg gatcagtaaa caaaacgatc ctaacgaagc gcgggcgcag
     5581 ctcatcacgc tgcgccgaag cgtgctaaaa ctaaccggtg atgttttgaa tgacgatccg
     5641 cgcgatctgc agctgctgcc cattgccgga cgcctgaaat gtgctcatgt aaatcatgta
     5701 gaagcgttat gcgacgcgga ggacgtgcta gtgtggtccg tggcggtcac gcccgcgatt
     5761 gaaaagctca gtgtctggga gttagatggc aaacacagct ggaaaagcct gccggatatt
     5821 cacagccgcg ccaacaatcc taccagccgg atgatgcgtt ttgcccagct ctctaccgtg
     5881 aagtcttttt cgccaaattg atagacaacc acaggagtca ttatgtctga aaaattgcag
     5941 gtggttacgt tactggggag cctgcgcaaa ggctcattta atggcatggt tgcacgtacc
     6001 ctgccgaaaa ttgctccggc gagcatggaa gtcaatgcgt taccttccat tgccgacatt
     6061 cccctgtatg acgctgacgt acagcaggaa gaaggttttc cagcaacggt tgaagctctg
     6121 gcggaacaga tccgtcaggc tgacggtgtg gtgatcgtca cgccggaata taactactcg
     6181 gtaccgggtg ggctgaaaaa tgccatcgac tggctttccc gcctgccgga tcaaccgctg
     6241 gcgggtaaac cggtgttgat tcagaccagc tcaatggccg tgattggcgg cgcgcgctgt
     6301 cagtatcacc tgcgccagat tctggttttc ctcgatgcaa tggtgatgaa caagccggaa
     6361 tttatgggcg gcgtgattca gaacaaagtt gatccgcaaa ccggagaagt gattgatcag
     6421 ggtacgctgg accacctgac cgggcaattg accgcatttg gtgagtttat tcagcgagtt
     6481 aagatctaaa taaaaaaccc gccagtaatc atgcatggcg ggtttttaat gcgctatcga
     6541 ttttaatgag cgtcgataaa tacaatcttc aggataaaca gcagcgcaac gatgattacg
     6601 cacgggctaa ggtcacgcag acgcccggta ccaatcttca tcacgcagta ggagataaag
     6661 cccagcgcaa taccttcggt aatcgaaaag ctgaacggca tcatcacggc ggtaataaac
     6721 gccggaacag attcagtaag atcctgccag ttcacgcgag caagacttga ggtcatcaac
     6781 acgccaacgt agatcaacgc gccagctgca gcgtagcctg gcaccattcc cgccagcggc
     6841 gacagaaaga taaccagcag gaacagcaga ccaacaacca ctgccgtcag accggtacga
     6901 ccgccaaccg atacgccgga agaggactca atataagccg taacggaaga agtaccgata
     6961 aacgaaccgg tcacggaaga gatactgtcg acatacagcg cctgcttcat gcgcgggaat
     7021 ttccccttct catccgccag acctgcttta tcggtcacgc caatcagcgt accggaggag
     7081 tcaaacaagt tgaccaacat gaaagagaaa atcacccctg ccagcccgag gttaaacgac
     7141 ccggctaaat ctacatgacc cacaactgtc attacgctcg gcggcgcaga aacgatgcca
     7201 ttgtagtgca catcacccag catccagccc agcagcgtcg tcaccacgat agaaaccagc
     7261 accgctgcgt gaatgttgcg cgaggccaga atagcaatga tgaagaagcc gaggataccc
     7321 agaagtacgc tgtgagaagt cagattaccg atgctcacca gcgtttccgg gttagcgaca
     7381 atcacacctg cgtttttcag ccccatcatg ccaatgaaca gaccgatacc gctggtaata
     7441 cccacacgca gactcaccgg aatgttggct atcatccagt agcgaacgcg gaaaatcgtc
     7501 agtaacagca gacctatcgc gccccagaag attgcgccca tcccgacctg ccacggcaag
     7561 cccatcgcct gtacaacgac aaaagcgaag aacgcattca ggcccatagc gggtgccagt
     7621 gcaactggca ggttagcaaa cagtcccatc ataatactgc cgaatgcagc gatcagacag
     7681 gtagtgacga agacggcgct ggtatccatg ccagcaacgc caagaatttg cgggttaaca
     7741 aaaacgatgt aaaccatcgt caggaaggtg gtaaaaccgg cgatcacttc ggtccgtgcc
     7801 gtcgtgccat gttcgcgcag tttaaacacg cgttccagca tcccctggcc agaagcctgg
     7861 gttgtgtgtt gttgactcat tatctatttc cgaacaagga gggaaaatcc gtcgctatcg
     7921 tataccaaaa tgagacaata tggacggctg aagataactt tttttcattg tatttgctta
     7981 tacggcaacg attgcgtcat gcgaattggc atgacgaaaa cgttaaacta acgataaagg
     8041 aaagacatgt cccagataga agcggtattt ttcgactgcg acggtacgct ggtcgatagc
     8101 gaggtcattt gctctcgcgc atatgtaacg atgtttcagg aatttggtat tacgctcgat
     8161 cctgaagagg tattcaaacg tttcaagggt gtaaaactgt acgaaattat cgatattgtt
     8221 tcccttgaac atggtgttac gttagcgaaa acagaagctg aacacgttta ccgtgcagaa
     8281 gtcgctcggc tgttcgattc agaactggaa gccatcgaag gggctggagc gctcctgtca
     8341 gcgatcactg cgccaatgtg tgtggtatct aacggcccaa ataacaaaat gcagcattct
     8401 atgggcaagc tgaatatgtt gcactacttc ccggataaac tgttcagcgg ctacgatatt
     8461 cagcgctgga agccagaccc ggcgttaatg ttccatgcgg caaaagcgat gaatgtaaat
     8521 gtagaaaact gcattctggt tgatgactca gttgccggtg cgcaatctgg tatcgacgca
     8581 ggtatggaag tgttctactt ctgcgccgac ccgcacaata agccgatcgt tcacccgaaa
     8641 gtcaccacct ttacccatct ttcgcagtta ccggaactgt ggaaagcgcg tggttgggat
     8701 attacggcat agttcttcac actcccttca cttaccccgc ttaaattggc gctcaaaggt
     8761 aagttaaggg tgtttgatat gtctgtttca cgtcgggtaa tacatcacgg actttatttt
     8821 gcggttttag gaccgttaat tggtgttctg tttcttgtcc tctacatatt cttcgcaaaa
     8881 gaaccgctgg ttcttttggt aataatacag gtacttccat tgtttctctt attgtcgatt
     8941 actacgggag ctattcctgc attgttaacc ggcgttatgg tcgcctgtct gccggaaaag
     9001 atcgggtcac agaaaaatta tcgttgtctg gctggtggca taggtggcgt cgttatcacc
     9061 gagatctatt gtgcagttat tgtacatatt aagggcatgg cttcctcgga gttgtttgaa
     9121 aacattcttt ctggtgacag tctcgttgtc cgcatcattc ctgcattgct ggcaggtgtg
     9181 gtgatgagca gaatcattac ccgtctgccc ggattggata tttcatgtcc tgaaacagac
     9241 tctttaagtt aagcgggata ctttatcttt gggctactca aaagcagaca ggatgttact
     9301 atgactcaaa atatcaggcc gttaccccaa ttcaaatatc atcccaagcc actggaaaca
     9361 ggcgcatttg aacaggataa aaccgtagag tgcgattgct gtgaacaaca gacgtcagtt
     9421 tattactcgg gtccctttta ttgcgttgat gaagttgaac atctctgtcc gtggtgtatt
     9481 gcggacggtt ctgctgctga aaagtttgca ggtagttttc aggatgatgc cagcatagaa
     9541 ggtgttgaat ttgagtatga tgaagaggac gaatttgccg gtattaagaa cacatatcct
     9601 gatgaaatgc tgaaagagtt ggttgaacgc acgccaggtt atcatggatg gcaacaggaa
     9661 ttctggcttg cgcattgtgg cgatttctgt gcttttatcg gctatgtggg ctggaatgat
     9721 ataaaagatc gcctcgatga atttgccaac cttgaagaag attgtgagaa tttcggtatt
     9781 agaaattctg atctagctaa atgcctgcaa aagggtggtg attgtcaggg ttatctcttc
     9841 cgctgtctcc actgcggcaa gctgagactg tggggtgatt tttcgtagtt atttaaataa
     9901 tgagaacagg ccggagcgta attcatacat ccggccttat ttcttcagct taaccgagcg
     9961 ctaattctgc ggcagcggct ttatcagcga ttaccatcag cgaaggatgc agctgcaaca
    10021 cggaagctgg aacatcttcg gtaaccggac cttgcagaac atttttcagt gcctgcgctt
    10081 tacctgcgcc actgacaatt atcagcaagt ttttcgccgc catgatgctt ttcggcccca
    10141 tcgtcacgta gctgtcaggc actaacgaaa agtcgccgcc caactcacca tgcgccacaa
    10201 tatcaaccat ctctccctgg atcgggaact ccacggtttg ctcatggaaa tgggtcgtat
    10261 tcggcaaatt gccacaaaaa tgaccatctg cacctaatcc cagcaccacc aaatccaggc
    10321 cgccttcacg cgccagtttc tgatcatgct cgcggtagtt atcaatagtg agcttctgga
    10381 tattctcttc tttgatcccc gcaggggtga aaaacagatt acgcagattg gtaatcgtta
    10441 caccttctcc ctctttgccg cgaaagggaa tttcatcgaa attatagaaa tagcagttat
    10501 cgtaccaggg cttaccttta accagggtgg tgaggtattc atacatgcct ttgggcgagc
    10561 taccggcggt aattgccagg ttaacacgac gcgtcgtcga catataacca agcagatggt
    10621 gtgccgcgac acggctcatt tcctggtaat cttcggtaat gattaatttc atcttagtgc
    10681 cttattttat ttaaataatt tctgtacgaa ggctgcatag gcaggccggg atattcctgg
    10741 taatcaaact taattttttt ctgctcaagc tcagttttca gcccggcgat atccttgccg
    10801 gttacgacat ctttatcacc aacgaccaca gtaaaattac gtagttgctg gttaatagct
    10861 gccggatcgt tcagccgggc cgcgacacct tcatccggta cggttgtcgt ggtaacacca
    10921 ctgaatgtgg ccagccagcc aaagctttcc agatgattca ttcccgatac cagcgcctga
    10981 tacccacctt gtgaaagccc tgccagtgcg cgaccatcgg catctttacg gacgttaaaa
    11041 cgcttgctaa tcagcgggat aatatcgttc atcagttcgc gatctgccgc tttagcattc
    11101 agcggataaa agactttacg tctttcctga ggcacgaaat cttcgggaat aatgcccttc
    11161 gcatcggttt cggtatctgg gatcaccacc agcatcggtt taattttccc ttcagccagc
    11221 aggttatcca tgatttgcgg gatacgcccc tgatcgatag cggaacgtcc agtatcacca
    11281 aagccgtgat agaaatagag cactggcaaa ggctcgccca tgccggtgta tcctggcggg
    11341 gtccagacat acatctgacg ttcagattgc aatgcgttgg agtggtaagt tatggcgatc
    11401 aaatcaccat gcgcaacaga acgcgtatcc agataactgc ctggcaccag aatcatactg
    11461 gagttaacct ggcgctgagg cttagtcatt gcggtgccag tatcaatgct gcgtacacca
    11521 tcaacattga aaaaatattc gtacagattg cctttcagga caggtgtgcg ccacgaccag
    11581 actcccgctt cgtctttggt catcgggtga atattgtccg gaaccggaac acccaccact
    11641 acggagacgt tttttgcccc aggggcaaag tagcgaaagg tcacgctgtt atcggcattg
    11701 acctgagtca catactgttt aaccggaata gtgggtgccg ggctggctgg catatccgct
    11761 gctatggccc actgagcaga aataccgctg gcaatagcca gcgttaaagc agcaattttt
    11821 atattcatcg taatattcct ttcattaagt tccattacca ccagatttca gcctgggcac
    11881 cgacagcaaa ctggtcgtct ttattgtctt caaaggtgaa gccatccagt tcgttttcca
    11941 gggctttgat atacgtggcg tagaaacgaa tttccggacg cgaggtcaac atactggtat
    12001 tgactttaaa ggtatggaag agcgtggttt tataaccaga ctcattaaat ttattactat
    12061 tcgcatcttt gttttgctgg gtgaaatagc ccagttcaac acctgtctgg ttatattggt
    12121 cccaaatata ggccgggcga acaaccgcac gaatagattc gaaatcagag tgggcgcctg
    12181 tttcgtagct atatatatcg ttaccgaagg agtaaacaat cgcgttagct acaatgaaat
    12241 gatcgccaat ataggcttcg ccctgcgaag tcagacgtac cgctgttccg ccggtgtgat
    12301 caccataata acgaccatta aaggtggtaa atggacttgc gccagcataa cggccaaagt
    12361 tactggcgat agaattattc gcgaccagga aggagaattc gttgaagcca cctttatcaa
    12421 atttctgcgt taaagatgtg ccaaacatcc aggtatcttt ccagtcataa tacccgttat
    12481 tatcctgatt atctttttcc gatgcgcttt cgtttgccgt gacataacga ccacttacca
    12541 ttaaggtggc tttatcccat aacgggatat ctttatagcg taaatcaatg gtattggtat
    12601 taatctgctg tttgttttgc aggctgcgat cgtaatcatc gacatcttcg cgaaccagcg
    12661 cgatatcaat tttacccgga ccgactttcc agttttccag acctacaccc gctgcggcat
    12721 cagtacgctg cgttttccag tcaagcatct ggatttcaat tttcggcgca ccgtgtttac
    12781 ccacccagaa atcagcctct ggcgcaaagg gcaggaaacc tttggtggta acgtacatat
    12841 cggagaactg catatagttc tcgccaccgg cgttatcgcc aaaccagccg gtagagtact
    12901 gctgaccaac gttaccatcc atcatcacaa cggcatcaac ccgtttgccg ttttcgttgt
    12961 agacacgttg ttttaactgc aaatcaaacc agccggagta ttcgttacca aagcggccca
    13021 gagaaccaat cgcccatgat ttaggtgaac catgagaggc ggtcccccaa ccagaacggt
    13081 aatagccgtt gtaactaaag ccaatttcat ctttcacaaa tttactgaaa tctttcagag
    13141 tcatggaaga ataaatcgag cctgtctcac tggcattttt ctcttcattt ttgaccagta
    13201 cagcatcagg ttttgcggca ctactggtcg ggaacggatt ggcggcatac ccttgatcat
    13261 tcgtacttac gctacgattc accgtcgctg gcgtatactt tttcttctct tcatctttat
    13321 actttttgag ttcgctttgc gtttctctta acgccttttc taataactca aggcgttgtt
    13381 ccaccgttaa tgattcagcc aatgattgtg cagaaaaggc taacggtgcc attaataaga
    13441 tggcagaggt aataagattt cgtctaaaca taatatccct ttatggtgca aagaaagaat
    13501 taacgcatcg catccagact gttctgaatg cgacgataat taaggtgctt taatggttat
    13561 tttttttaat gacagccccc gcgtcttgat cacctctgca taccatccga agcttttctt
    13621 gcgtgttctt gtgaggcttc cttcgccatt atcatcacga tccacataaa taaagccgta
    13681 gcgcttagac atttgtgaat gagatgcact gactaaatca attggccccc aactggtgta
    13741 ccccataata tccacaccat cggcaatcgc ttcatttacc tgtaccaggt gatcgtttaa
    13801 ataggcaatt cgataatcgt cctgtatcga accatccgct tcaacgctgt cttttgcgcc
    13861 taatccgttc tcgacaataa ataacggttt ttgataacga tcccaaagcg tatttaacag
    13921 aacccgtaat ccaaccggat caatttgcca cccccactct gaacttttca gatgcggatt
    13981 ggggatcata ttcagtatgt tgccctgcgc atttttatta atgctttcgt cgtgggaaac
    14041 acaaccagtc atgtaataac taaaagagat gaaatcgacg gtatgtttta aatcttctgc
    14101 gtcactttca gtcatctcaa tggtgatatt gtggtcgcgg aagaaacgct gcatatagcc
    14161 gggatactgg ccacgcgcct gaacatcacc aaagaacatc cagcgccggt tctcttccat
    14221 ggcctgcaac atatcctgtg gctggcaggt gagggggtaa accagcccac cgagaagcat
    14281 attgccgatt ttcgcttcgg ggagcaggct atgacaggct ttaactgccc gcgcactggc
    14341 aaccagttga tggtggatag cctgataaac ttccgcctcg ccactctctt ctgccagccc
    14401 cacgcccgtg aatggcgcgt gtaacgacat gttgatttca ttaaacgtca gccataacgc
    14461 cactttatgt tggtagcgag taaagaccgt gcgggcgtaa tgctcgaagt gatcgatgac
    14521 cgctcgatta gcccaaccgc cgtagttttt caccagccca tatggcattt cgtaatggga
    14581 taacgttacc agcggcttga tccccgcctg cgccatttca tcaaacagcc gatcgtaaaa
    14641 cgctaacccc gcttcattcg gttcgacttc gtcgccctga gggaaaattc gcgcccaggc
    14701 aatggaaata cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta
    14761 acggtgataa aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc
    14821 catttttccc attacgccat gaggctgtaa atctgaggtc gagatccctt tgccatcttc
    14881 ctgccaggca ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg
    14941 aaatgctttc ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc
    15001 tgcgcttatc tgcgccgtgc cgtggggtaa tacgtccgta aaatcatcgc tattactgat
    15061 taataccggc gtcgtcagat caaatccggc ctcgcgaata gcagggatat caaaagaaat
    15121 cagccgatcg cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc
    15181 gtccagtttt acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc
    15241 aatgccaatg gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg
    15301 cacttcacca accgagggca gaatggcaat acctttaccc aacaggccac tggcaaacgt
    15361 ggtatcagcg acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc
    15421 ctgctcaggt ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg
    15481 acgtttagcg gtgatgaaat gaagcatcac cgtaccgaca aatgcgcaac cgatggcaat
    15541 gacaccgcca ataacgctgg cccagacggt gaaatcaatt cccgttgacg ggatggtttg
    15601 catgaaggtg aaaatacttg gcaaaccaaa ggagtagact ttcgtttgcg cgtagccaat
    15661 gatggtggcc cccaaagccc cactgataca ggcgataaca aaggggtact tacgcggcag
    15721 gttgacgcca tataccgctg gttcggtgat accaaacaga ctcgtcaacg ccgctgatcc
    15781 cgccaccact tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac
    15841 ctgcgccata atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa
    15901 gttattgata cacagcggca ccaggcccca gtgcagtccg aacatgacga agatttgcca
    15961 gaagccgccc attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc
    16021 ggcggcaatc agttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg
    16081 tgtgataacc atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca
    16141 cgcattaagt cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac
    16201 cgatgacgag taattcaaca atgtgaccgg aatacccagg aaatccagcc ccagcgcatc
    16261 cgctttttgc ccgttctcga aagcagtcag aattaatgga tgcactaacg ctccaccaat
    16321 caccatggca gtaaatggat taccgccgaa gcgtttcccc gcggtgtatc ccaggattat
    16381 cgggaagaac caaaacaagg catcactggc gctgaataaa attaaataag taccactttg
    16441 ttcggtcgtc cactgaaaag tgagcgccag agccagcata cctttcaaga tcccggttgc
    16501 cgccatcaaa ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag
    16561 cagattacct ttatcatcat tttccggtgc ctgttgcgct ttttcgtcaa ggcctgccac
    16621 actgttaacc gccaggaaga catcggccac atggttacct atgaccacct gaaactggcc
    16681 accgctttcc accaccataa taataccggg ggtctttttc agtacctctg cttgcgcttt
    16741 gctttcatcc tttaatttaa aacgtaatcg cgttgcgcaa tgcatcagac tcacaatgtt
    16801 atctgcgccc ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct
    16861 accgctttgc ggcaaaactc caaaaaaaaa cctgaaaaaa acggcctgac gtgaatcaag
    16921 caattttttt caggttttgc ccgcttagtg cggtaacaat cctttactca gtaataatat
    16981 ttcagtgttc tttgcgcacg cgctctatat ttatggctaa aaacataatc tctgcgggtg
    17041 aaattttacg ttgatactgc aaaccaataa aaatggcgat ccgttccgca cattgccatg
    17101 cttgcgggta attttgtttt actgcttgtt gtaatgattc atcactatcg ttaattgaag
    17161 catgttcaag aatacgccag gataaaaact tcagatgtgt aaccagtcgc tgataactca
    17221 agctttcttc ctggtaatta aggctgaact gaaattttat taattgcagc atttcgcgca
    17281 ttaactgcgt gacacctgca acatcctcca tatttccgct catttgggca ctgaccagat
    17341 gcatggcaat aaagcccact tcatctttcg gtaactgcac gcccaaccgt ttatcaataa
    17401 tggttaacgc ttcttcccct agctggaact ctttcgggta aagccgctgg atatcccaca
    17461 gcaacgggtt gggcagcaac acgttttgct gaaagcgttt aatcgcaaac tggcaatggt
    17521 cagttagcga gatataaata ctgtcctgta attttcccaa gcgctcctgc gctaaagaga
    17581 taatacgatc acaggttgcc atcacctcaa gaggaatatg acttaagagt tcgcttaatc
    17641 gcccgttcag ttcatgactg ctcaaggcat actctttttc tattccactt gagttaattc
    17701 tttcgccagc gcgtttttga aagccaattc cgcgccccat gacgactttt tcccgctgtt
    17761 gatcatcaat aaccaccaca acattattgt tgagaatttt ggtgatttgc atgttcatag
    17821 caaggacctt ttttataaac aaaaaaaccc gacttcacca gtattctctg gttatgtcag
    17881 gttttgcctg cgaatgcagt aacaatccag tcatttatta atggttttta taacgaacat
    17941 ccaggttcgg aaattaattt aattgcgtgc agagaaaata gcaatgcgct attgataaaa
    18001 atatg
//
LOCUS       DAPHBJ010000088        17563 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.089, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000088 DAPHBJ010000000
VERSION     DAPHBJ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.089"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            84..1223
                     /gene="arnB"
                     /locus_tag="RHZ76_002596"
     CDS             84..1223
                     /gene="arnB"
                     /locus_tag="RHZ76_002596"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HDV2813213.1"
                     /translation="MSEFLLFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIAMTQLAKLEYLNTRRREIALQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRNALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1227..2195
                     /gene="arnC"
                     /locus_tag="RHZ76_002597"
     CDS             1227..2195
                     /gene="arnC"
                     /locus_tag="RHZ76_002597"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HDV2813214.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2195..4177
                     /gene="arnA"
                     /locus_tag="RHZ76_002598"
     CDS             2195..4177
                     /gene="arnA"
                     /locus_tag="RHZ76_002598"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HDV2813215.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPDVIFSFYYRHLIYDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4174..5064
                     /locus_tag="RHZ76_002599"
     CDS             4174..5064
                     /locus_tag="RHZ76_002599"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HDV2813216.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5064..6716
                     /gene="arnT"
                     /locus_tag="RHZ76_002600"
     CDS             5064..6716
                     /gene="arnT"
                     /locus_tag="RHZ76_002600"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HDV2813217.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPNFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6713..7048
                     /gene="arnE"
                     /locus_tag="RHZ76_002601"
     CDS             6713..7048
                     /gene="arnE"
                     /locus_tag="RHZ76_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HDV2813218.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7048..7434
                     /gene="arnF"
                     /locus_tag="RHZ76_002602"
     CDS             7048..7434
                     /gene="arnF"
                     /locus_tag="RHZ76_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HDV2813219.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPMTKQRY"
     gene            complement(7428..7694)
                     /gene="pmrD"
                     /locus_tag="RHZ76_002603"
     CDS             complement(7428..7694)
                     /gene="pmrD"
                     /locus_tag="RHZ76_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HDV2813220.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(7804..9159)
                     /gene="menE"
                     /locus_tag="RHZ76_002604"
     CDS             complement(7804..9159)
                     /gene="menE"
                     /locus_tag="RHZ76_002604"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDV2813221.1"
                     /translation="MIFSDWPWRHWRQVRGETIALRLNDEQLNWRELCARVDELASGF
                     AVQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARVTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9156..10118)
                     /gene="menC"
                     /locus_tag="RHZ76_002605"
     CDS             complement(9156..10118)
                     /gene="menC"
                     /locus_tag="RHZ76_002605"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDV2813222.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETREEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRDRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10118..10975)
                     /gene="menB"
                     /locus_tag="RHZ76_002606"
     CDS             complement(10118..10975)
                     /gene="menB"
                     /locus_tag="RHZ76_002606"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDV2813223.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10990..11748)
                     /gene="menH"
                     /locus_tag="RHZ76_002607"
     CDS             complement(10990..11748)
                     /gene="menH"
                     /locus_tag="RHZ76_002607"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDV2813224.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWVQRFLTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11745..13415)
                     /gene="menD"
                     /locus_tag="RHZ76_002608"
     CDS             complement(11745..13415)
                     /gene="menD"
                     /locus_tag="RHZ76_002608"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDV2813225.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIDGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13504..14799)
                     /gene="menF"
                     /locus_tag="RHZ76_002609"
     CDS             complement(13504..14799)
                     /gene="menF"
                     /locus_tag="RHZ76_002609"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HDV2813226.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAVVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14878..15183)
                     /gene="elaB"
                     /locus_tag="RHZ76_002610"
     CDS             complement(14878..15183)
                     /gene="elaB"
                     /locus_tag="RHZ76_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HDV2813227.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLCSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15238..15699)
                     /locus_tag="RHZ76_002611"
     CDS             complement(15238..15699)
                     /locus_tag="RHZ76_002611"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV2813228.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15764..16681
                     /gene="rbn"
                     /locus_tag="RHZ76_002612"
     CDS             15764..16681
                     /gene="rbn"
                     /locus_tag="RHZ76_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HDV2813229.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16869..>17563
                     /locus_tag="RHZ76_002613"
     CDS             16869..>17563
                     /locus_tag="RHZ76_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HDV2813230.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDKFGKEAEDILYHIVRTPTNETLSIIRLIKNACLKLYNLAHTATN
                     SPLKPAGPDNSDDLLFKKLFSPSKLMTIIGDEIPLISEKQSLSKVLLNDKNNELSDGT
                     NFWDKNRQLTTDEIDCYLQKIAANAKNTEVNYPTGLYVPYSTRTHLEDTLNENIKSDP
                     SWPKAVQLFPINTG"
BASE COUNT         4128 a         4733 c         4422 g         4280 t
ORIGIN      
        1 tttcgctcca aatggcaaaa tatacacaac actctttata gcaaatataa gtggacaggt
       61 attcaatggc ggaaggaaaa gcaatgtcag aatttttgct tttttcgcga ccagcaatgg
      121 gcgtggagga actcgctgca gttaaagagg ttctcgaatc cggttggatc acaaccggcc
      181 cgaagaatca ggcgcttgag caagcctttt gccagttgac gggaaatcag catgccatcg
      241 cggtcagttc agccaccgcc ggaatgcata tcacgctaat ggcgttggaa attggcaagg
      301 gtgatgaagt gattacgcct tccctgacct gggtttcaac tctcaatatg atctccttgt
      361 tgggtgcaac gccggtaatg gtggatgtcg accgcgatac gctgatggtc acgcctgaag
      421 ctatcgagtc agccattacg ccacgcacta aagccatcat tccggtgcat tatgccggtg
      481 cgccagcaga tattgacgcc attcgcgcca ttggcgaacg ttacggcatc gcagttatcg
      541 aagatgcagc ccatgccgtc ggtacgtatt acaaagggcg acatattggc gcaaaaggta
      601 ccgctatttt ttcgtttcac gccatcaaaa atattacctg tgcggaaggt ggcctgattg
      661 ttactgataa tgaaaatctt gcccgccagc tacggatgct gaaatttcac ggtctgggtg
      721 tcgatgccta tgacagacaa acctggggcc gtgcaccgca ggctgaagtc ttaacgccgg
      781 gctataagta caatctgacc gatattaacg ccgcgattgc catgacacag ttagccaaat
      841 tagagtacct caacacccgt cggcgcgaaa ttgccctgca atatcagcaa gcactggcag
      901 ctctcccctt tcagccatta agtcttcccg cctggccgca cgttcacgcc tggcatctgt
      961 ttattattcg tgtcgatgaa caacgttgtg gtatcagtcg aaatgcgttg atggaagcgt
     1021 taaaagaaag aggcattggt accgggttac atttccgcgc cgctcacaca caaaaatatt
     1081 atcgcgagcg ttttcccacg ctgtcgttac cgaataccga atggaatagc gaacgcatct
     1141 gttctttgcc gctgttcccg gatatgacca ccgccgatgc cgaccgtgtc atcacagccc
     1201 ttcagcaact cgcaggacaa taagccatgt ttgaaatcca ccctgttaag aaagtctcgg
     1261 tggttattcc cgtttataac gagcaggaaa gcttaccgga attaatcagg cgcaccacca
     1321 cagcctgtga atcgttgggg aaagagtatg agatcctgct gattgatgac ggcagtagcg
     1381 ataattccgc gcatatgctg gtcgaagcct cacaagcgga gaacagccat attgtgtcta
     1441 ttttgcttaa ccgcaattac gggcaacatt cagcgattat ggcgggattc agtcacgtta
     1501 ctggcgactt aattattacc cttgatgccg atctccagaa tccgccagaa gaaatccccc
     1561 gcctggtggc aaaagccgat gaaggttacg acgtggtagg gactgtacgc cagaaccgcc
     1621 aggacagctg gtttcgtaaa accgcttcga agatgattaa ccggcttatt cagcgcacca
     1681 ctggcaaagc gatgggtgat tacggttgta tgctgcgcgc ctatcgccgt catattgtcg
     1741 atgcgatgtt gcactgccat gaacgcagca cctttatccc gattctggcg aatatcttcg
     1801 cccgccgtgc cattgaaatt ccagtacatc atgccgagcg tgagtttggt gaatccaaat
     1861 acagttttat gcgcctgatt aatttgatgt acgacctggt gacctgcctt accacaacgc
     1921 cgctacgtat gctgagtctg ctcggcagca ttattgcgat tggaggtttt agcattgcgg
     1981 tgctgctggt gattttacgc ctgaccttcg gaccacaatg ggcggcagaa ggcgtcttta
     2041 tgctatttgc cgtgctgttt acttttattg gcgctcagtt tatcggcatg ggattactcg
     2101 gtgaatatat cggcaggatc tacaccgatg tccgcgcccg cccccgctat tttgttcagc
     2161 aagttatccg tccatccagc aaggaaaatg aataatgaaa accgtcgttt ttgcctacca
     2221 cgatatggga tgcctcggta ttgaagccct gctggctgcc ggttacgaaa ttagcgccat
     2281 ttttacccat actgataatc ccggtgaaaa agccttttat ggttcggtgg ctcgtctggc
     2341 ggcggaaaga ggcattccgg tttatgcgcc ggataacgtt aatcatccgc tgtgggtgga
     2401 acgcattgcc caactgtcgc cagatgtgat tttctctttt tattatcgcc atcttattta
     2461 cgacgaaatt ttgcagctcg ctcccgcagg tgcatttaat ctgcatggtt cgctgttacc
     2521 aaaatatcgt ggtcgcgcgc cgctgaactg ggtgctggtc aacggtgaaa cggaaactgg
     2581 cgttacattg caccgaatgg tgaaacgtgc cgatgccggg gccattgtgg cgcaactgcg
     2641 cattgccatt gcgccagacg atatcgctat tacgctgcat cataaattgt gccatgccgc
     2701 gcgccagcta ctggaacaga cattacccgc cattaaacac ggtaatattc tggaaatcgc
     2761 ccagcgcgaa aacgaagcca cctgttttgg tcgcagaacg ccggatgaca gtttccttga
     2821 atggcataaa ccggcatccg tactgcacaa catggtacgt gccgttgccg atccgtggcc
     2881 gggtgccttc agctatgttg gcaatcagaa attcaccgtc tggtcgtcgc gtgttcatcc
     2941 tcatgccagc aaagcacagc cggggagcgt gatttctgtt gcgccactgc tgattgcctg
     3001 tggcgatggc gcgctggaaa tcgtcaccgg acaggcgggc gacggcatta ctatgcaggg
     3061 ctcgcaatta gcgcagacgc tgggcctggt gcaaggttca cgcttgaata gccagcctgc
     3121 ctgcaccgcc cgacgccgta cccgggtact catcctcggg gtgaatggct ttattggcaa
     3181 ccatctgaca gaacgcctgc tgcgcgaaga tcattatgaa gtttacggtc tggatattgg
     3241 cagcgatgcg ataagccgtt ttctgaatca tccgcatttt cactttgttg aaggcgatat
     3301 cagtattcat tccgaatgga ttgagtatca tgtcaaaaaa tgtgatgtcg tcttgccgct
     3361 ggtggcgata gccacgccga ttgaatatac ccgcaacccg ctgcgcgtat ttgaactcga
     3421 ttttgaagag aatctgcgca ttatccgcta ctgcgtgaag taccgtaagc gaatcatctt
     3481 cccgtcaact tcagaagttt atgggatgtg tagcgataaa tacttcgatg aggaccattc
     3541 taatttaatc gtcggcccgg tgaataaacc acgctggatt tattcggtat caaaacaatt
     3601 acttgatcgg gtgatctggg cctatggcga aaaagagggt ttacagttca ccctcttccg
     3661 cccgtttaac tggatgggac cacgactgga taaccttaat gcagcgcgaa ttggcagctc
     3721 ccgcgctatt acgcaactca ttctcaatct ggtagaaggt tcaccgatta agctgattga
     3781 tggcggaaaa caaaaacgct gctttactga tattcgcgat ggtatcgagg cgttataccg
     3841 cattatcgaa aatgcgggaa atcgctgcga cggtgaaatt atcaacattg gcaatcctga
     3901 gaacgaagcg agcattgagg aactgggcga gatgctgctg gcgagcttcg aaaaacatcc
     3961 gctgcgccat catttcccac cgtttgcggg ctttcgcgtt gtcgaaagta gcagctacta
     4021 cggcaaagga tatcaggacg tagagcatcg taaaccgagc atccgcaatg cccaccgctg
     4081 cctggactgg gagccgaaaa ttgatatgca ggaaaccatc gacgaaacgc tggatttctt
     4141 cctgcgcacc gttgatctta cggataaacc atcatgacca aagtaggctt acgcattgat
     4201 gtcgatacct ttcgtggcac ccgtgaaggc gtgccgcgtc tgctggaaat cttgagtaag
     4261 cataatattc aggccagcat ttttttcagc gtcggcccgg acaatatggg ccgccatctc
     4321 tggcgactgg tgaagccaca gtttttgtgg aagatgctgc gctcaaacgc ggcatcgctt
     4381 tatggctggg atattttact ggcaggtacg gcctggccag gtaaagagat tggtcatgcc
     4441 aatgccgata tcattcgtga agcggctaaa catcacgaag tcggcctgca cgcctgggat
     4501 caccatgcct ggcaagcccg tagcggtaac tgggatcggc aaacaatgat cgacgatatt
     4561 gcacgcggtc ttcgcactct ggaagagatt atcggtcaac cggtaacctg ttctgccgct
     4621 gcgggctggc gtgccgacca gaaggtgatc gaagcaaaag aagcgttcca tttgcgctac
     4681 aacagcgatt gtcgtggggc catgccgttc cgtccattgc tcgaatcagg aaaccctggc
     4741 actgcgcaaa ttccggtgac cttacccacc tgggatgaag tgattggtcg ggatgtgaaa
     4801 gcagaagatt ttaacggttg gttactcaac cgcatcctgc gagataaagg cacgccggtt
     4861 tataccattc atgcagaagt tgaaggctgc gcttatcagc ataattttgt ggatctcctc
     4921 aaacgcgcag ctcaggaagg cgtgacattt tgccctttaa gcgaactgtt atcagagacg
     4981 ttgccgctcg gacaagttgt tcgcggaaat attgccggac gtgaaggctg gctgggttgc
     5041 caacaaattg cgggtagtcg ctgatgaaat cggtacgtta ccttatcggc ctcttcgcgt
     5101 ttattgcctg ctattacctg ttaccgatca gcacgcgtct gctctggcaa cccgatgaaa
     5161 cgcgttatgc ggaaatcagt cgagaaatgc tggcatccgg cgactggatt gtgccccatc
     5221 tgttagggct acgttatttc gaaaaaccca ttgccggata ctggattaac agcattgggc
     5281 aatggctatt tggcgcgaat aactttggtg tgcgggcagg cgttatcttt gcgaccctgt
     5341 taactgccgc gctggtgacc tggtttactc tgcgcttatg gcgcgataaa cgtctggctc
     5401 tactcgccac agtaatttat ctctcattgt ttattgtcta tgccatcggc acttatgccg
     5461 tgctcgatcc gtttattgca ttctggctgg tggcgggaat gtgcagcttc tggctggcaa
     5521 tgcaggcaca gacgtggaaa ggcaaaagcg caggattttt actgctggga atcacctgcg
     5581 gcatgggggt gatgaccaaa ggttttctcg cccttgccgt gccggtatta agcgtgctgc
     5641 catgggtagc gacgcaaaaa cgctggaaag atctctttat ttacggctgg ctggcggtta
     5701 tcagttgcgt actgacggtt ctcccttggg gactggcgat agcgcagcgg gagcctaact
     5761 tctggcacta ttttttctgg gttgagcata ttcaacgctt tgcactggat gatgcccaac
     5821 atagagctcc gttctggtac tacgtgccgg tcatcattgc cggtagcctg ccgtggctgg
     5881 gattactccc cggtgcactg tacacaggct ggaaaaaccg caagcattcc gcaaccgtct
     5941 atttgttgag ctggacgata atgccgctgc tgtttttctc cgtcgctaaa ggtaaattgc
     6001 ccacctatat tctttcctgc tttgcatctc tggcaatgct gatggcgcat tacgctttgc
     6061 tggcagcaaa aaataatcct ctggcgctgc ggattaatgg ctggattaac atcgcttttg
     6121 gcgtcactgg cattattgcc acatttgtgg tctccccgtg gggaccaatg aacacgccgg
     6181 tgtggcaaac cttcgagagc tataaagtct tttgtgcctg gtcgattttt tcgctatggg
     6241 catttttcgg ctggtacacc ttaacaaacg tcgaaaagac ctggcctttt gccgcgcttt
     6301 gcccgctggg gctggcgttg ctggtaggat tttcaattcc tgacagagtt atggaaggaa
     6361 aacatccgca attttttgtc gagatgacac aagaatcact tcagccaagc cgctatattc
     6421 ttactgatag cgtcggtgtt gccgcaggtc tggcatggag cctgcaacgc gatgacatca
     6481 tcatgtatcg ccagacaggt gagttgaaat acggccttaa ttatccggat gcgaaaggga
     6541 gatttgtcag cggtgatgag ttcgcaaact ggcttaatca acatcgtcag gaggggatta
     6601 ttactctcgt gctttcggtt gaccgcgatg aagatatcaa cagtctcgcc attccgcccg
     6661 cagatgccat cgatcgtcag gagcgtctgg tgctgattca gtatcgtccc aaatgatctg
     6721 gctaacatta gtctttgcca gcttgcttag cgttgccggg cagttgtgtc agaaacaggc
     6781 aacctgcttt gtggcgataa acaaacggcg caaacatatc gtgctgtggc tgggactggc
     6841 gctggcttgt cttggtcttg ccatggtgct ctggctgctg gtcttgcaga acgtaccggt
     6901 aggcattgcc tacccgatgt taagtctgaa ttttgtctgg gtgacgctgg ctgcagtaaa
     6961 actgtggcac gaaccggtat cgccgcgtca ctggtgtggg gtggcgttca ttattggtgg
     7021 cattgtgatc ctcgggagta cggtgtaatg ggcctgatat ggggattatt cagcgtcatt
     7081 attgcctcag ttgcgcaatt aagcctgggt tttgcggcaa gtcatctgcc gccgatgacg
     7141 cacttatggg attttatcgc ggctctgtta gcttttggcc tggatgcccg gatactgtta
     7201 ctcgggctgc tgggttatct gctttccgtt ttttgttggt ataaaacgct tcataaactt
     7261 gcgctaagca aagcctatgc cttgctcagt atgagttatg tgctggtatg gattgcttca
     7321 atggtacttc ccggctggga agggactttt tcgctgaaag cactactggg agtagcctgt
     7381 attatgagcg ggttgatgct gattttcctg cccatgacaa aacaacgtta ctgagttttc
     7441 cctgccactt tacactgccg ttcccactca tcggggctat aactgcttgc cgagaggact
     7501 ttcaccgtat gcagcttttc acgattaata caataaagcg cattctgcaa aggcgagagt
     7561 aaatccccca ctttcacggc gaagtcactc ttcacttcgg cgatcatttt tatcgcgccg
     7621 ccagcatcgc agagcataag tacatgtctg ttatcttgtt tattgcagca cgattttttg
     7681 accagccatt ccattgcatt atcctgtttg ctaagagttt tcacatcaat ttacagatta
     7741 acagataaac tctttatcaa cggcaatgga atggctgaaa tgagaagtga aacggagggt
     7801 taattattgc tgacgttgca cccactcttt tagcgcctga cgtgaaattt taataccgcc
     7861 gtttttcagt tcaggcggca acgttaacca acgcaccggt tgctgaaaac gggccagctt
     7921 atctttcacc cattcactaa gatcaacgct ctcgtggtca tactccatca ccgccaccgg
     7981 tcgatgacca aactccttgt cggcaacggg gacgataaac acctgcaaaa ccgcaggatg
     8041 tgcagcaatt acgcgctcga cttcttccgg ctgaataccc tctccgccac tgaagaatag
     8101 attgtctaaa cgtccgacaa tggtcagctt gccattatgc atctcaccgc gatcgcgcgt
     8161 agcgtaccag ccttcgtcat taaccagtga aaccagttgc ccgttacgcc agtaaccttc
     8221 tgccatactg gcagcccgca gccacacttc attattaacg attttcactt cccgacccgg
     8281 cagcggcgaa ccaacgtctg ccaggccgtc ggcttctttc gcacacaccg tggaggcaaa
     8341 ctcggtcaga ccatagccgc aaaagcaacg aatcccctgc tcgcgcgcct gttccgtcaa
     8401 ctcgaccggg atagccgcgc cgccaagtaa caccgctttc agggaaacgg aactgcggtt
     8461 aaccagcaaa cgccagagtt gtgttggcac cagtgaagcg tgagtacagc ctgccagcat
     8521 ttgctccaat ggctgtttat cacgcaccgt cacccgcgca ccagcgtata accagcgcca
     8581 cataattccc tgaccggaga cgtgaaacag cggtaaagag agcaaccagt catcgtgatc
     8641 gccaaacgga atcaacgata acaccccttc ggcactggca agatgggcct gataagtatg
     8701 gacagcggct ttcggcaacc cggtagaacc cgaggtcaac gtcattgagc acagacgcgt
     8761 cggctgccac gtagcggcat gtgcgccttc aacccgctga atgtgcagcg acgttaatgc
     8821 cggaaacgtg ttttccccat ccggcaccag agcaaattgc agcgtcagat tgggcagcaa
     8881 ttcttcaagc aacggttgcg gcagctgagg gttcacgggc aacacccgcg ccccgcattg
     8941 cagtaacgcc agccaggcga gcagcgtttg cggcgtattc cacgcccgca acatcacgcc
     9001 gctgccctca accaccccct gcaccgcaaa tccggaggct aattcatcga cgcgagcaca
     9061 aagctcgcgc cagttgagtt gctcgtcatt aagacgtaag gcgatggttt ctccccgcac
     9121 ttgccgccag tgacgccacg gccagtcaga gaagatcata acaaccgctc cagtgcatca
     9181 acttccacga caggcagcgt gctacccggc cagcgacgta cctgctgcgc ctgcatcaga
     9241 tccagcgtgt ccagccctgg aatggtgtcc ggcgttaacc aggcggcaat ccgcgccagt
     9301 tgcgttaagc ctaagctcga ttcaatggaa gaactgatca ccgccgtcag ccccagcgcg
     9361 tgcgccgcct gtacctgctc gcgtactttt tccagactgc ccgtgagcgt gggtttgata
     9421 actaccgcgc gcacgccctc ttcagccaca aaggcaaaat ccggctcgcg caggctttca
     9481 tcccaggcaa tggcaatgcc ggtttcacgg gcaaacgctc gcgaatcatc gcgggttttg
     9541 cacggctctt cgagaaacgc gatgcggtcg cgataatccg ggttaacgta tttggcaaac
     9601 tgctgacctt tcagcggtgt ccaggcgcga tttgcgtcaa gacgcaaatg cagatccgga
     9661 attgcctcca gcaacagatt caccaccatg ccgtcgcgca ccgcttcgta caatcccact
     9721 ttgaccttcg ccactttctc gcctggcata tctgcaagtt tgaggatcag atcgtccgga
     9781 tcgccattac acagcggtgc cgcacggtag ttggctgctt gcggcaacgt atctgtcagt
     9841 tctgccaatg cacagcttac gccaaaggcc acggaaggca tctgcggtag ctcgcaatcg
     9901 cctgccagcc agttatttac ccaggcaagc agcacacttt gcgcctcttc cctggtttcc
     9961 tgactgaagc ccggcagtgg ggagatctcc ccccaccctt cgcgctcgcc ttcacgcagg
    10021 caaacataca gcccgtcgcg ggtttttaac cgcctgtcgc gcagaaccac ccccgcgtcc
    10081 atggggatct gccagcggta tacctgcgcg ctacgcatta cggattccgt ttgaatttgc
    10141 tgaagtcagg ctgacgtttc tggttgaagg cgttgcgacc ttcctgacct tcttccgtca
    10201 tgtagaacag catggtggcg ttgcccgcca gctcctgcag ccccgcctgc ccgtcacagt
    10261 cggcgttcag tgcagctttc aggcagcgca gcgccatcgg gctgttttgc agcatttcgc
    10321 ggcaccaacg gacggtttct ttttccagat ccgccagcgg taccacggtg ttcacaaggc
    10381 ccatatccag cgcctgtttt gcgtcgtact gacggcacag gaaccagatt tcacgcgctt
    10441 ttttctgccc gacgatgcga gccatgtagg aagcgcccca gccgccgtcg aaggaaccga
    10501 ctttcgggcc agtctgaccg aagatggcat tatctgccgc gatagtcagg tcgcacatca
    10561 tgtgcagaac gtgaccgccg ccgatggagt agccagccac catcgcgaca accggtttcg
    10621 gacaggtacg gatctgacgc tggaagtcca gcacattcag gtgatgtacg ccggaatcat
    10681 ctttatagcc gccgtaatca ccacgcactt tctggtcacc accggagcag aacgctttat
    10741 cgcctgcacc agtcagaatg atcacgccga tgttgtcgtc ataacgcgca tctgccagcg
    10801 cctggatcat ctcttttacc gtcagaggac ggaaggcatt gcgcacctgc ggacgattaa
    10861 tggtgatttt tgcgataccg tcggtggatt tttcataacg aatgtcctcg aaaccttcgg
    10921 agcagtcgtg ccattcaacc ggtgcgtaaa gcattgcttc atcaggataa atcatattgt
    10981 gtcctttagt cagaaacgca agatctgcgc cagacttgcg attacgccag cgggattttc
    11041 ccgatgcgcg ttatgtccgg cgcgaggaat gacatggcag tcggcagcca gttccgccgc
    11101 cagggcgcgg aatttgctgt cacgttcacc acataaataa taaaacgcaa atgtgcgggc
    11161 gctaaggtta gcacgtaaat caggctggac ggcgagagaa gtcgcctcca gcatggcggc
    11221 aagcgttgcg ccattattgt tgctgcgcag cgccaccagc tcccggcgtt gatcgtcatt
    11281 gagtgaggca aaaacaggct gttgatacca gtcggcaaat accgccgtta acggttctgt
    11341 gagaaaacgc tgcacccatt ggcgatcgga acgctgacgt tccgcacgtt gttcagcatt
    11401 ttgcagcccc ggatgcccgc cttcgacaat aaccccacaa agccccgcca gcccctggca
    11461 agccgccatc atcgccaccc gtccaccaag cgagtacccc accagccaga agtcaaggat
    11521 gttgtaacta accaaggttt tacgcagtaa gtcggtgaca tcatcaaatc catcgacgct
    11581 aatcgccgcc gaaccaccgt gacctgggag atcaacatac aaccgtgagt agtcggcaaa
    11641 cgcctcgccc acttcttgcc attcgtggca atcgccggaa aaaccgtgca aaaacaccag
    11701 ccagggtaaa cctggttttc cgtgttttgc ctgcgcgtgc aggatcataa atggcttacc
    11761 tgcgccagaa gttgctggag cgtttgcgca ccatcggtgt cgttaaccac catttcaatc
    11821 accgtggtgg ttggcgtgcg ccaggcgtcg gcaaatgccg tttcaagttc ctgccagttt
    11881 tgcggacgat gatatttcag ctcgaacatc gcggcggcgt gctcaaaatg gacgttttgc
    11941 ggcatcagat agaaacgctc acgctcgctt tgcggcgttg gcaacagcga gaaaatttgc
    12001 ccgccgttgt tgttcaccac aattaatacc agcggcgcag aaacctgacg caataacgcc
    12061 agcgcgttga gatcgtaaag tgcggagaga tcgcccacaa tcgccagcgt cggtttgccg
    12121 cttgcccgct gaacgcctgc ggcggtcgaa agcagtccgt cgataccgct ggcccctcgg
    12181 ttgctgtaca ccgggtaacc tgccggaagt tgcgaaagcg catcaatcag acgtaccacc
    12241 aggctgttac caacaaacaa ttgcccctgt tcaggcagat agtcgcagat gcgatgcgcc
    12301 agttgcgctt cgccaaacgc atcacggcgg gcaataaccg cctgcattgc ctgttccgcc
    12361 aggcgcggga tttcaacgca ccagggctgg cgtttttctg ccggatgcag ctccagccag
    12421 tcggcaatat tggcaattaa gcgacgtccg cgatggtgtg ccggatcaag tcgcccgtca
    12481 atgtcatcaa caatccagta ctcttctggt tcacagcttg cctgccattg caggagccgt
    12541 ttccccgtca ggctgcttcc cagttgcacc acaatttgcg cctgctgcaa ctcgctggtc
    12601 gctttggcat tgcctaacca aagatcggca cacggcagcg gctgcccggt ttgtgacagc
    12661 acatcgccaa tcagcggcca gccaagagtt tgcgcccaca gggcaacttt tttgccctct
    12721 tccgcactca tgcgcccggc aaccaccacg ccgcgctttt gtcgccagaa gaaccagtcg
    12781 cgctgttttt cactttccag acgaggcgct tcacgcagcc acggtttgtc gtcctgccac
    12841 cagtcaccca gacgctgttg ccagctaagc ccggtatcgt ccatttcgcc atacagcggt
    12901 tcagcaaacg ggcagttgat atggactccc cccgcatgaa gcgtaccgag agcgtggtcg
    12961 atggtagaaa ccagccaacg tgcggggata tcctgggtcg ggcgcggcaa tgaaatactg
    13021 tgcgtggggt gagaggcgaa cattcccggc tggcgaattg cctgattcgc gccgcagtca
    13081 attagctccg gcgggcgatc ggcggttaag agaatcagtt tttctccggt taacccggct
    13141 tcaatcagtg ccggatagag atttgccacc gccgtgccgg aggtcacaat caccgccacc
    13201 ggctgcttgc tgactttcgc cagccccagc gccagatgcc ccaacccacg ctcatcgaaa
    13261 tgggtgtggt gaatgaatgc ggaattctcc gccgccgcta acgttaacgg tgtagaacgc
    13321 gagcctgggg cgatacagat gtgtctgacg ccgtgacgcg ttaatgcttc cagaatgacc
    13381 gccgcccagc gtcggttaaa tgcgcttact gacatgagtt tgtccggtat caatattgcg
    13441 gctaagtata aggagctaca aaaatagatt attgatatga atcggtaatg atgcgactca
    13501 ttactattcc atttgtaata aagtacgcag ccctgccgct ttgttgtcga tttcctgcca
    13561 ctcttgctcg gggtcggaac cacggacaat gcccgcgcca gcatataatc gcacgacatt
    13621 gccgctaatt tttgctgagc gcagggaaac gcagaattcg ctttgttgta atgagagata
    13681 gcccgcagaa ccggcgtacc attctcgggt gaacggttcg tggcgggcga taaactgtcg
    13741 cgccagatcg cgcggtaagc cagcaactgc tgccgttggc tgcaactgat gtaaacagat
    13801 cacatcatcc gctttgttga gtgaagtcca gatacagcgg cgaagatgct gcactttacg
    13861 cagacgcagt acctgcggcg gtaaaacatc cagcgtctgg gtatcggcct gtaatcgttg
    13921 acagatatct tccaccacca gcatgttctc gcgctggttt ttatcatccg ccatcagcca
    13981 ctctcctaac tgctgcgcct gcttatcatc aggattattt gctactgttc ccgccagcgc
    14041 ttcagtacgc agcgctttgt cacgccgccg ccataaccgt tccggtgaag agccaagaaa
    14101 agcattttcg ccatcaaagg ccatgtaaaa atggtagcaa ttcagattca gtcgacgact
    14161 ggcagccatc atcgccgccg cgttgaccgg acttgcgaaa tgcaggtcag ttgcccgagc
    14221 gagcaccact ttgtcgagct caccttcggc gatggttttc gttgccagtt cgattaattg
    14281 cgtccagccc gttttgtccg gccagtgttg ttctcgcgtg gtggttaaat gtaacccagg
    14341 caagggcttg atactcacca gtgtggcgat aaattctttt gcctgaatcg catcgtgctg
    14401 aagggagctt tcgctgaata gcgtcagccg cagcgtggct ttaccgccac agcgtcgcca
    14461 ttccaggcgg ggtaaaagta aattgccctg cgacgggtca aaagcattca gcccccaaat
    14521 gcgtaagtcg gcgtgttccg ggtgctggcg aagaaaacgt tgtgcctggt ccaacgacgt
    14581 aaaacgggta atcgcgccca ggacgacagc ttcttcatca ccattacgtt gttgccagta
    14641 gaattgcggg tatgtttgct gactggccag ccagctcaag gcatcaaaag cgtctttgag
    14701 agggaaagga atatcgataa cccgaatgcc gggtgtcgcc ggaatctctt gcgacaaatg
    14761 gcgcagtaga ttttccagcg ccgtagtaag tgattgcacg cggacctctc cctgttaaaa
    14821 gcctcacatt atacggggta ctacaaaaaa aatgcagtac cccggtgtag ggaggtttta
    14881 acggcgtgcc agcaacagtc ctagtaccag cccaacggcc gcgcccacac caattccttg
    14941 ccagggtttt tcgtggacgt agtcatcagc acgataaact gcctgcttcg cccgatagta
    15001 ataactgtct gaagcctggc taacccgttt tttcacatca tccagcgctt tttctgcacg
    15061 cgctttcagc tcaacatatt tctgatcggc gggatcgcca gaggagcaga gcacctcttc
    15121 cagtgtttca ctaagcagcg tcaggtcgtc atcgatacgt gtatcaccaa actgattaga
    15181 cattctcgtt ctccatttgc gtaaaacctg tcactaacta tagacaaggg ttgttgatta
    15241 cgcctgaatt acctcgcgtg ccatcccaat gtgtgggata ccatcttctt catagacctc
    15301 cgtcacaggg ataaaaccga aactctggta gaagttttgc agatgcgcct gcgccccgag
    15361 gtaaacaggc ttatcaggcc agtgatgcgt acatgtttcg agtgttttac tcatcagctg
    15421 ctgccccact ttttcgccac gcaacgcttc actgacaatc acccgaccta taacgaccgg
    15481 ctcaagatca tcatcacttt tcagaatcct cgcatacgcc accagttcat catttttcca
    15541 accgagaata tggcgattat cccccgtcaa atcatcacca tcgatatcct gataagggca
    15601 attctgctcg acaacaaaca ccgcgcaacg cagttgtaat aaggcataaa gctgggaaac
    15661 cgataattca gagtgatgca gatcttgcca ttcaatcata atgctctcct tttagatgta
    15721 tgccttatac tagccggaat attttttgaa acgtgatgaa ctcatggaat taattttttt
    15781 aggtacttca gccggtgtgc caacccgcac gcgcaatgtc acggcaatat tgcttaacct
    15841 gcaacatccg acccagagcg gactttggtt gtttgactgc ggtgaaggca cccagcatca
    15901 gctactgcat accgccttta accctggaaa actggacaag atttttatca gtcaccttca
    15961 tggcgatcat ctttttggtt tacccggctt gctgtgcagt cgttctatgt caggcattat
    16021 ccaaccctta acgatttatg gtccgcaagg tatccgtgaa tttgtggaaa ccgcgctgcg
    16081 gattagcggc tcatggaccg attatccgct ggaaattgtc gaaattggcg ctggcgaaat
    16141 tctcgatgat ggcctgcgca aagtaaccgc ttatccgctg gaacacccac tggaatgtta
    16201 tggctatcgt attgaagaac atgataaacc gggtgcatta aatgcccagg cattaaaagc
    16261 tgctggcgtg ccgcctggcc cactgtttca ggaattaaaa gcgggcaaaa caatcacgct
    16321 ggaagatgga aggcagatta acggcgcaga ttacttagct gctccagtgc caggtaaagc
    16381 gctcgctatt ttcggcgata ccggcccctg cgatgccgca cttgacctgg ctaaaggtgt
    16441 cgatgtcatg gtgcacgaag cgacgctgga tataaccatg gaagccaaag ccaatagtcg
    16501 cggccatagc tctacacgcc aggctgcgac actagcccgt gaggctggag tcggcaagct
    16561 aatcattacc cacgtcagct cgcgctatga tgacaaaggt tgtcagcacc tgttacgtga
    16621 atgcaggtca attttcccgg cgactgaact ggcgaatgat ttcaccgtgt ttaacgttta
    16681 actctgctta ttattaaaaa cagggcgaaa cttgccctgt tatcgcaacc cgcgctacat
    16741 atatccaccc acacaggcat attgagcgat ttttgatatt tattcatctc cttacgcact
    16801 atattcagag caataagttg cctcttttca tcttgattca gcacaacctt tgcaactaaa
    16861 aggagcccat gatggttaca gttgtcagca attattgtca attatctcaa acgcaactca
    16921 gtcagacatt tgcagaaaaa tttactgtga ccgaggaatt actgcagtct ttaaaaaaaa
    16981 cagcgttatc cggagatgaa gaaagcattg agttactgca taatattgcg ttaggttatg
    17041 ataaatttgg gaaagaagct gaagatattc tttaccatat tgttagaacc ccaacaaatg
    17101 agaccctatc gattatccga cttatcaaaa atgcttgttt aaaattatat aatttggcac
    17161 ataccgcaac caactccccc cttaaaccgg ctggtccgga taattcagat gatctcctgt
    17221 tcaaaaagct attctcccct tcgaaattaa tgacaattat cggtgatgaa attcctctta
    17281 tatctgaaaa acagtcgctt tcaaaggtgc ttttaaatga taagaataat gaactgagcg
    17341 atgggacaaa cttctgggat aaaaatcgtc aattaaccac agatgaaata gattgctatc
    17401 ttcagaagat cgccgctaat gcaaaaaata ctgaagtcaa ttatcctact ggtctctacg
    17461 tcccctactc caccagaact cacctggaag acactctcaa tgaaaatatt aagagcgatc
    17521 catcatggcc gaaagcagtc cagttattcc ccataaatac tgg
//
LOCUS       DAPHBJ010000089        17546 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.090, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000089 DAPHBJ010000000
VERSION     DAPHBJ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.090"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            63..479
                     /locus_tag="RHZ76_002614"
     CDS             63..479
                     /locus_tag="RHZ76_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traK protein"
                     /protein_id="HDV2813231.1"
                     /translation="MPIITAKVSDELLAYIDLVSGGNRSDYLRRCLEAGPGDRESGLK
                     IVADRLSDVNRKLDYLFDRASDADFGPLRDELKAITETLSGVKFPPAGQMMLHESLAI
                     ETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER"
     gene            481..2010
                     /locus_tag="RHZ76_002615"
     CDS             481..2010
                     /locus_tag="RHZ76_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HDV2813232.1"
                     /translation="MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYL
                     VKNTFSLWPLWSALIAGWFIGVGGLIAFIIYDKSRVFKGERFKKIYRGTELVRARTLA
                     DKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKTTIFNELLFKSIIRGGKNIAL
                     DPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQESPDMATEE
                     WFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEK
                     AVGSARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWL
                     DSIFSIVLGMGEKESRINVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLV
                     KVYGRDMAQTILANMRSNIVLGGSRLGDETLDQMSRSLGEIEGEVERKESDPQKPWIV
                     RKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKAKHVKYHRKNPVPGIELRE
                     I"
     gene            2010..5246
                     /locus_tag="RHZ76_002616"
     CDS             2010..5246
                     /locus_tag="RHZ76_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HDV2813233.1"
                     /translation="MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEAL
                     GLSGDVESARFKELLVGEIDTFTHMQRHVGDAKKERLGYDLTFSAPKGVSMQALIHGD
                     KTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTNNLVVATFRHETSRALDPDLH
                     THAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYELRYNSKNNT
                     FDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREE
                     IHQEWASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSL
                     SERDASFERQKLIQIANKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGAS
                     VMAETLTRKEWIDSLTNSGMRADKARFAVDDGIKNGRLKKTSHRVTTVEGIRLERSIL
                     TIESRGRGQMPRQLTAEIAGQLLSGKTLKKEQMRAVTEIVTSKDRFVAAHGYAGTGKS
                     YMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLDEKSVVF
                     IDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMK
                     DIQRQKNEVLLEAVKYAAEGNAACALKNITGVNELKEEAPRLAQLADRYLSLSSEQQD
                     ATLIISGTNASRKTLNDYIRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIII
                     PEQDYKNGMKRGESYQVLDTGPGNKLTVESISGEQIAFSPRTHTKLSVYQAVSAELAP
                     GDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVELDKKQASYLSYAYATT
                     VHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVSRDSP
                     KTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGT
                     DYQPVSNAEDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYE
                     HYAEASDYDFDSSIYDDYAMPQTSQAEQSHTGKEHTHEHEHEEGGHEI"
     gene            5246..5590
                     /locus_tag="RHZ76_002617"
                     /pseudo
     CDS             5246..5590
                     /locus_tag="RHZ76_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FipA"
     gene            5744..5872
                     /locus_tag="RHZ76_002618"
     CDS             5744..5872
                     /locus_tag="RHZ76_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813234.1"
                     /translation="MRYLLDIVSTDGYYWYMSGKICERVSDYRTAAFFEIGRLLTL"
     gene            complement(6046..6579)
                     /locus_tag="RHZ76_002619"
     CDS             complement(6046..6579)
                     /locus_tag="RHZ76_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HDV2813235.1"
                     /translation="MKKLATWLLAAAFTTAALPAFAVEPSVQVGYSPEGSARVLVLSA
                     IDSAKTSIRMMAYSFTAPDIMKALVAAKKRGVDVKIVIDERGNTGRASIAAMNYIANS
                     GIPLRTDSNFPIQHDKVIIVDNVTVETGSFNFTKAAETKNSENAVVIWNMPKLAESFL
                     EHWQDRWNQGRDYRSSY"
     gene            complement(6579..7574)
                     /gene="tadA"
                     /locus_tag="RHZ76_002620"
     CDS             complement(6579..7574)
                     /gene="tadA"
                     /locus_tag="RHZ76_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDV2813236.1"
                     /translation="MTDAAFYQLGPLREYLEDPTVFEIRINCFQEVICDTFSGRRVVQ
                     NAAITADFIRNLAKSLVSSNKLTMQAINDVILPGGIRGVICLPPAVIDGTTAVAFRKD
                     LAADKNLEQLTSEGIFSDCRKITGSKQSLTDDDFFLKELHSSEKWPAFLQTAVEKKRT
                     IVICGETGSGKTVLTRALLKSLHKDERVIILEDVHEVTVDHVVEAVYMMYGDAGKIGR
                     VSATDALRACMRLTPGRIIMTELRDDAAWDYLKALNTGHPGGVMSTHANSARDAFNRI
                     GLLIKATPIGRMLDMSDIMRMLYSTIDVVVHMEKRKIKEIYFDPEYKMQCVNGSL"
     gene            complement(7616..8776)
                     /gene="virB10"
                     /locus_tag="RHZ76_002621"
     CDS             complement(7616..8776)
                     /gene="virB10"
                     /locus_tag="RHZ76_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein VirB10"
                     /protein_id="HDV2813237.1"
                     /translation="MARKSVDVDQELDENTGDGEFESERGGFNGSNRRSAPGMKAFVI
                     LMALLALVFIGITVMGKIRAPAKAEADKDGGKAQQANTLPNYSFNSDPDVNKPATAQN
                     SATDARAVQAAAQADADAGSSNTGARTSNKRKEPSPEELAMQRRLGGELAQTNQAATS
                     NSPGAQPQDNETSEGSSALAKNLTPARLKASRAGVMANPSLTVPKGKMIPCGTGTELD
                     TTVPGQVSCRVSQDVYSADGLVRLIDKGSWVDGQITGGIKDGQARVFVLWERIRNDQD
                     GTIVNIDSAGTNSLGSAGIPGQVDAHMWERLRGAIMISLFSDTLTALVNQTQSNNIQY
                     NSTENSGGQLASEALRSYMSIPPTLYDQQGDAVSIFVARDLDFSGVYTLADN"
     gene            complement(8776..9660)
                     /locus_tag="RHZ76_002622"
     CDS             complement(8776..9660)
                     /locus_tag="RHZ76_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HDV2813238.1"
                     /translation="MKKLLLSAVVLSVLGGAATNVMALEVGRNSPYDYRIKSVVYNPV
                     NVVKIDAVAGVATHIVVAPDETYITHAFGDSESWTFAHKMNHFFVKPKQAMSDTNLVI
                     VTDKRTYNIVLHFIGEETKKNADGTVSKSFIETPWAVRHAVLQLTYEYPFEQQEKAKS
                     AADKKRITQKLKQTAFAGAKNYQYVMSEQPEMRSIQPVHVWDNYRFTRFEFPANAELP
                     QVYMISASGKETLPNSHVVGENRNIIEVETVAKEWRIRLGDKVVGVRNNNFAPGAGAV
                     ATGTASPDVRRVQIGEDN"
     gene            complement(9671..10369)
                     /locus_tag="RHZ76_002623"
     CDS             complement(9671..10369)
                     /locus_tag="RHZ76_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004216210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HDV2813239.1"
                     /translation="MKANKKTGLTREAIKEFNESRKGLEVDLMDEVLKSRRTAWMVAT
                     GSAVVTVFALSLVGYVVHKYSQPIPAHLLTLNEATHEVQQVKLTRDQTSYGDEIDKFW
                     LTQYVIHRESYDFYSVQVDYTAVGLMSTPNVAESYQSKFKGRNGLDKVLGDSETTRVK
                     INSVILDKPHGVATIRFTTVRRVRSNPVDDQPQRWIAIMGYEYKSLAMNAEQRYVNPL
                     GFRVTSYRVNPEVN"
     gene            complement(10359..10505)
                     /locus_tag="RHZ76_002624"
     CDS             complement(10359..10505)
                     /locus_tag="RHZ76_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HDV2813240.1"
                     /translation="MRSLLLMGVLLISACSSGHKPPPEPDWSNTVPVNKTIPVDTQGG
                     ANES"
     gene            complement(10588..11628)
                     /locus_tag="RHZ76_002625"
     CDS             complement(10588..11628)
                     /locus_tag="RHZ76_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HDV2813241.1"
                     /translation="MAFTLVQDIFAKVDGAITSMVSANVATIISDVTPLVATCLTIKL
                     MMQGLYSMFNPGAGDSLSSLIKEYLSIALILSFATAGGWFQQDLVNVALHMPDDFAGI
                     LSSPDKVEASGVPAIIDSGIEKGVRIVNTAWDAADVFSSSGLAAYAIGGIMLVATVVL
                     GGLGAGFVIMAKILLAVTLCFGPIAIFCLLWGPTKNIFARWLGSVINYGLVVVVLALV
                     FGFIMQMFDNLLSSMNSDAAYSSITGSMAALLLTIISIFVLFQIPQVAQSWGSGISAG
                     VADAARSTGSSMQALGNMGSHGMFGGNAFRGGNTGGGQQSAGGGSGSNSGGSSGSNLS
                     GKARGSRGKKAA"
     gene            complement(11644..11871)
                     /gene="eexN"
                     /locus_tag="RHZ76_002626"
     CDS             complement(11644..11871)
                     /gene="eexN"
                     /locus_tag="RHZ76_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncN-type entry exclusion lipoprotein EexN"
                     /protein_id="HDV2813242.1"
                     /translation="MKKLLLLIPFFLVACDASHDVDWYKKHDKERKATIEECKKNADE
                     LQKPDCKNAREADRQLFVHGKEDGEINSPKI"
     gene            complement(11879..12592)
                     /locus_tag="RHZ76_002627"
     CDS             complement(11879..12592)
                     /locus_tag="RHZ76_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HDV2813243.1"
                     /translation="MKKSLTAVLLTTGLILGGAQSASAGIIVSNPTELIKQGEQLEQM
                     AQQLEQLKSQLETQKNMYESMAKTTNLGDLLGTSTNTLANNLPDNWKEVYSDAMNSSS
                     SVTPSVNSMMGQFNAEVDDMTPSEAIAYMNKKLAEKGAYDRVMAEKAYNNQMQELSDM
                     QALTEQIKSTPDLKSIADLQARIQTSQGAIQGEQAKLNLMNMLQQSQDKLLRAQKDRA
                     TRNFVFGTGGDVTASPSIN"
     gene            complement(12610..15210)
                     /locus_tag="RHZ76_002628"
     CDS             complement(12610..15210)
                     /locus_tag="RHZ76_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="HDV2813244.1"
                     /translation="MRAATATKPKKIDAYRKEPSVNKKYLPYSYHLNDYVISMENGDL
                     MAFFKLDGRTHDCASDRELVTWHKDLNTLVKSFGTDHVELWTHEYHHEAKEYPDGEYD
                     HFFPAYIDQYNRKLHGDSKQLINDLYLTVIYKQVGDKTQKFLAKFEKPTRDEIQQMQN
                     EALEGLEDISEQILEAMKPYGIQQLGIYYRDKRGVEIPAPDKKEREELAEVDESDIFD
                     EAIVIERNEPEPSQAHAYSKALEFLYFLANMEWAIVPVCRDRIREYIMDNRPVSSLWG
                     DVVQIRTVDHNFYTTGIEFREYEEDTEPGQLNMLKEADFEYLLTQSFSCLSESSAKTF
                     LTHQEKSLQETRDRAQSQLAQLGTALDMLTSREFVMGYHHGTVHVWDNDQNAVQRKAR
                     RVKVMLTGCGVVGGTLSLASEAAYYARLPGNQKWAPRPVPINSWNFLHFSPFHNFMRG
                     KPDNNPWGPALTMFRTISGTPLYFNFHVTPLEELSYGKRPLGHALITGMSGEGKTTLL
                     NFLLAQSMKYNPRLFVYDRDRGMEPFIRSVGGYYKVLQQGMPSGFAPLQIEPTKRNIA
                     LIKNLFRICVETTNNGPISATMATELAEGVDAVMGEGSLIPREARTVTILDGYVNEVV
                     ENGVSLKGLLREWTREGQYGWLFDNDKDSLDLSANDIFGFDLSEFIAAKEEVSSPART
                     PLMMYLLYRVRDSIDGKRRVIQCFDEFHAYLDDPVIEREVKRGIKTDRKKDAIYVFAT
                     QEPNDALSSRIGRTIMSQTVTKICLRDPEAIREDYAFLTDAEYDALMSITEHSRQFLV
                     KQGQQSAIASFNLYPRNSDDIDADIKTMDNVLSVLSGEPQNAEIAHELVERLGNDPEV
                     WLKEYWRLTA"
     gene            complement(15210..15527)
                     /locus_tag="RHZ76_002629"
     CDS             complement(15210..15527)
                     /locus_tag="RHZ76_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000496058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HDV2813245.1"
                     /translation="MFVDGKRPLFKGATRLPRALGVPRNVAMMIFMISASLFMIIHMW
                     AILVFVFLWIPSAALTKYDDRMFRIMGLWLKTKFSNWFDSPFKQWGGSSYSSVDYKRK
                     GLK"
     gene            complement(15577..15870)
                     /locus_tag="RHZ76_002630"
     CDS             complement(15577..15870)
                     /locus_tag="RHZ76_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraM"
                     /protein_id="HDV2813246.1"
                     /translation="MTTLFKKYGPAVVMGVLSIALPQIALAAGTDTGESTATSIQTWL
                     STWIPIGCAIAIMVSCFMWMLHVIPASFIPRIVISLIGIGSASYLVSLTGVGS"
     gene            complement(15880..16161)
                     /locus_tag="RHZ76_002631"
     CDS             complement(15880..16161)
                     /locus_tag="RHZ76_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV2813247.1"
                     /translation="MERLYLVLQSQILELFEAGKVKEVTIERVSLKKWYPVFQIDDEQ
                     LGQIACSIRVNKEHELRTWADLRLLAEFLKDKCGVEECRLNLQSTEDSE"
     gene            complement(16170..16904)
                     /locus_tag="RHZ76_002632"
     CDS             complement(16170..16904)
                     /locus_tag="RHZ76_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDV2813248.1"
                     /translation="MSKHPKLLVLALACLACAGRASAAPASDEVARLAQRCAPDVSPL
                     TMAYIVGHESSNGPYRININGSIQLKQQPRTEAEAVSVAKVLLKDNKSFDMGLAQINS
                     NNLVGLGLSVDDIFKPCINLRASQTILKACYDSALKSYPAGQVALRHALSCYNTGSLT
                     NGISNGYVTKVINVARQSTDLKIPTLLPDGQTSEDSTANEPQQAKSTAPQYDGEQDVF
                     GSGDGDAFSRNNTDAFLTRQETAKGE"
     gene            16968..17318
                     /locus_tag="RHZ76_002633"
     CDS             16968..17318
                     /locus_tag="RHZ76_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HDV2813249.1"
                     /translation="MSRRNEKKTAEGRKVMLVPLKSEKRPKGEPVYRDPDNPFNTWTG
                     IGKRPAWLTAKLDAGISLEAMKMQGVANPREHRPAKYRDPRNAENTWSGTGRRPTWLK
                     ELLDSGLSLDDLKI"
BASE COUNT         4439 a         4441 c         4334 g         4332 t
ORIGIN      
        1 ctaagatttc agcttgttag atcccaatct tttgtttaag aaatgcacaa tgtgaggact
       61 gaatgccaat aataaccgca aaagtatcag atgaactttt ggcctatata gacctggttt
      121 caggaggtaa tcggtcagat tatctgcggc gctgccttga agctggccca ggtgatcggg
      181 agtccgggtt gaaaatagtt gccgaccggc tgagcgacgt aaatcgcaaa ctggattatc
      241 tttttgaccg tgcttcagat gcggattttg gcccactgcg cgatgagctg aaggcaataa
      301 ccgaaacgct atctggcgta aaattcccac cggcaggaca gatgatgttg catgaatcac
      361 tcgctattga aacgctaatc ctcctgcgct cgatagcaga accgggcaaa accaaagcgg
      421 caaaagcaga ggttgaacgt aacggctaca aagtctggga accaaaaaag gagcgctgaa
      481 atggacgata gagaaagagg cttagcattt ttatttgcaa ttactttgcc tccagtgatg
      541 gtatggtttc tagttgcaaa atttacctac ggtattgatc catccacggc taaatacctg
      601 attccgtatc tggttaagaa tactttttcg ctatggcctt tatggtcagc tttaattgca
      661 ggctggttta ttggtgttgg cggtctgatc gcttttatca tttatgataa atcacgcgtg
      721 tttaaaggcg aaagattcaa aaagatttat cgtggtacag agcttgttcg cgccagaaca
      781 ctcgctgata aaacacgcga aagaggtgtc aaccagttaa ccgtggctaa tatccccata
      841 cctacatacg ctgagaactt gcatttttcg attgccggta caaccggtac tggtaaaacc
      901 acaattttca atgaactgtt atttaagagc atcattagag gcggcaaaaa tattgcttta
      961 gatccaaatg gggggttctt aaagaatttc tatcgtcccg gcgatgttat tttaaacgcc
     1021 tatgataaac gcactgaagg ctgggtgttt ttcaatgaaa ttcgccgttc atatgattac
     1081 gagcgcttag tgaactctat tgttcaggaa agccctgata tggctactga agaatggttc
     1141 ggctatggcc gtcttatttt tagtgaagtt tcgaaaaaac ttcacagcct atacagcaca
     1201 gtaactatgg aagaagttat tcactgggcc tgtaacgttg accagaaaaa attaaaagaa
     1261 tttttaatgg ggacgcctgc cgaagctatt ttttccgggt ctgaaaaagc agttggaagc
     1321 gcgcgatttg ttctcagtaa gaatcttgcc ccacatttga aaatgccgga aggtaatttt
     1381 tccctgcgtg actggcttga tgatggaaag ccgggaaccc tgtttatcac ctggcaggaa
     1441 gaaatgaaaa ggtcacttaa tccgctaatt tcctgctggc tggattcgat tttttctatc
     1501 gtgctgggta tgggtgaaaa agaaagccgc attaatgtat ttattgacga gctggaatca
     1561 ctccagtttc tgccaaacct caacgatgca ctgaccaaag ggcgtaaaag cggtctgtgt
     1621 gtttatgctg gctatcaaac ctattctcag ctggttaagg tttatggtcg ggatatggct
     1681 cagaccattc tggctaacat gcgttctaac atcgtgctgg gcggcagccg tctcggtgat
     1741 gaaacgttgg atcaaatgtc gcgctcactc ggtgagatag aaggcgaagt tgagcgtaaa
     1801 gaatccgatc ctcagaagcc ctggattgtc cgtaaacgcc gcgacgttaa agttgttcgt
     1861 gccgtaacgc ctaccgaaat atcaatgttg ccaaacctca ccggctatct ggcgttgcct
     1921 ggtgatatgc ccgtcgctaa gttcaaggct aaacacgtta aataccatcg caaaaaccct
     1981 gttcctggca ttgaactgag ggagatctga tgcttgatat aaccacgatt acccgccaga
     2041 acgtcactag cgttgtgggc tactactctg atgcaaagga tgattactac agtaaggatt
     2101 catcattcac gtcctggcag ggaaccggag ctgaagccct cggcttgtcc ggggacgttg
     2161 aatcagcccg gtttaaagag ctgcttgtcg gtgagataga taccttcacg catatgcagc
     2221 gacacgtggg tgatgccaaa aaagagcgtc tgggctacga cctgacgttt tcagcgccta
     2281 aaggggtatc catgcaggcg ctgatacacg gcgataaaac cattatcgag gctcatgaaa
     2341 aagctgttgc cgctgctgtg cgtgaagctg aaaaactcgc gcaggcccgg acgactcgcc
     2401 aggggaaatc agtaacccag aataccaata accttgtcgt cgctactttc cgccacgaaa
     2461 cgtccagggc gctagatcct gatttgcaca cacacgcttt tgtcatgaac atgacccagc
     2521 gcgaggacgg ccagtggcgc gcgctcaaaa atgatgagct gatgcgtaac aaaatgcacc
     2581 tgggcgatgt ttacaaacag gagctggcgt tggagttgac caaagccggt tatgagctgc
     2641 gttacaacag caaaaataac acgttcgata tggcccattt ttcagatgaa cagattcgcg
     2701 ctttttcccg ccggtcggag caaattgaaa agggactcgc tgcaatgggt ctgacgcggg
     2761 aaaccgccga tgctcagacg aaaagccgcg tctcgatggc tacgcgtgaa aagaaaacag
     2821 agcattcccg tgaagaaatt caccaggaat gggccagccg cgccaaaacg ctcggcattg
     2881 attttgataa ccgtgaatgg cagggacacg gtaaacctct ggaggctgat atagcgcgca
     2941 acatggcccc ggattttacc agcccggagg ttaaagctga ccgggctatc cagtttgcag
     3001 ttaagtcgct gtcagagcgc gatgccagct ttgaacgcca gaagctgatt caaatcgcta
     3061 ataagcaggt gctgggccat gccacaatag ccgatgttga aaaagcgtac ctgaaggcgg
     3121 tacagaaggg cgccatcatc gagggcgaag cccggtatca atcaactctg aaggtcggtg
     3181 cttcagttat ggccgaaact ctgacgcgca aagagtggat cgactcgctg acgaatagcg
     3241 ggatgcgtgc ggataaagcc cgttttgcgg tagatgatgg tattaaaaac ggcaggctta
     3301 aaaagaccag ccaccgcgtc actaccgtgg agggtattcg ccttgaacgt tccattctga
     3361 ctatcgaatc acgcggccgg gggcagatgc cgcgacagct gaccgcagag attgcaggcc
     3421 agctgctttc cgggaaaacc ctcaagaagg agcagatgcg cgcggttaca gaaattgtga
     3481 cgagcaaaga tcggtttgtg gccgcacatg gttacgccgg taccggtaaa agctatatga
     3541 cgatggccgc taaagagctg ctggagtcac aagggctgaa ggtcacggcg ctggccccct
     3601 atgggacaca gaaaaaagcg cttgaagatg atggattacc ggcccgcacc gttgccgctt
     3661 tcctcaaagc gaaggataaa aagctggatg aaaaatcagt cgtattcatt gatgaagccg
     3721 gggttatacc cgcccgacag atgaaacagc tgatggaggt aatcgagaag cataacgctc
     3781 gcgcggtatt cctgggggat acgtcacaga cgaaagcggt agaagccggt aagccttttg
     3841 agcagctgat taaagcaggt atgcagacca gctacatgaa agacattcag cgacagaaga
     3901 atgaagtttt gcttgaggcg gttaagtatg ccgctgaagg taatgccgcg tgcgccctga
     3961 aaaatatcac cggcgtgaac gaactgaagg aagaagcgcc ccgactcgct caacttgccg
     4021 atcgctacct gtctctgtcc tcagaacaac aggatgccac cctgataatc tctggtacga
     4081 acgcctcacg caaaaccctc aatgactaca tccggggcaa tctggggctt gccggaaccg
     4141 gcgaaacgtt cacgctactt gaccgcgtgg attcgacaca ggcggaacgc cgcgacagcc
     4201 gttatttcag taaagggcaa atcattatcc ccgaacagga ttacaaaaat ggtatgaagc
     4261 ggggcgagtc ttaccaggtt cttgataccg ggccgggcaa caaattgacg gttgaaagca
     4321 tcagcggtga gcagatcgct ttcagcccgc gtacacacac caagctgtct gtataccagg
     4381 cagtcagcgc cgaactggcg ccgggcgata aggttatggt aacgcgtaac gataaaacgc
     4441 tggacgttgc caacggcgac cgcttcacgg tgaaaaccgt ggagggtgaa aaactgacgc
     4501 ttgaggacaa aaaagggcgc acggttgagc tggataaaaa acaggcttct tatctttcct
     4561 atgcttatgc aaccaccgtc cacaaatccc aggggcttac ctgtgatcgc gtgttgttca
     4621 acatagatac caaatcgctt accacttcta aggacgtttt ttatgttggt atttcgcgtg
     4681 cgcgtcatga ggtggaaatt tttaccgacg ataaaaaatc tttggcatcg agcgtgagcc
     4741 gcgacagccc gaaaaccacg gccgcagaaa ttgaccgttt ctttgggctt gaggccaggt
     4801 ttaaggatat tggccgcgat accagtttag aaacccgatc agctgaaaaa ggtctgccag
     4861 aagccacggg ggaaagcatg gcgtttaacc agaaacccga tgaacataac atgacaaccg
     4921 gcactgatta tcagccggtg agcaacgcag aggacgcctt tcatcttaag cagaacccta
     4981 tggatgattc cgttggcctg cggcgtcatg aagcacagca aaatgatgca gagctggccc
     5041 atgattatgc tgctgccgac gatcagcagt ggagcgcgca ggagtatgcc gattacgagc
     5101 attatgccga agcatccgac tacgactttg acagcagcat ttacgacgat tacgccatgc
     5161 cgcagacgtc tcaggcggaa cagagccata ccggaaaaga acacacccat gagcatgagc
     5221 atgaagaagg gggccatgaa atctgatgga gcagatcgac aagcgcaagc aggataaact
     5281 gaagttcgac cgggtaatta atctggcgca cagactgccg caaccggcga tccatgactt
     5341 actacgcgcg ctgatactgc caattcaggc cgattacttg ctggccgttg gcacggaggg
     5401 ccaggacgcg cgcccggaca tgaacgagcg cgagttcttc ttcacaaaaa tcatctgggc
     5461 gatggattac acgcacatga aatcactcag gcttgcagct gaagattttc ccctggcgct
     5521 tgcgacggcc aagatcctgc cgtggccgtg gggtgaatca agctaccgga gtgctttagc
     5581 cgatataggc agcgctaagg gcaatccgtg ggtacaggat attaaccacc gcgttacttt
     5641 gtggttgccc tggcggatag gctttgtacg aggagggaat cattctattg ccagcggcgt
     5701 tctggccggt gagggtgagg taatacccga tacggtttat gatatgcggt atttgctcga
     5761 tattgtcagc actgacgggt attactggta catgagcggg aagatctgcg agagagtcag
     5821 tgactaccgt acagccgcct tttttgaaat aggccgcctg cttacactgt gattccttca
     5881 aaatatctca acgaaatttc ccgacacctg tttaagctat cttgctgata tttctgtttt
     5941 tcgatttttg ccggcgcgat gccggcgacg ttggccagct gccccgggcg cgatactgag
     6001 atcagtttct ttgaacaaaa aaaaccgcct tttggcggtc agtattcagt agctggaacg
     6061 gtagtctctt ccctgattcc agcggtcttg ccagtgttcg aggaatgatt cagcgagctt
     6121 aggcatgttc cagatcacca ccgcattctc cgagtttttc gtttcggccg ctttggtgaa
     6181 attaaagctg ccagtttcaa cggtcacatt atccacgatg atcaccttgt catgctggat
     6241 agggaaatta ctgtcagtac gcaaagggat gccgctgttc gctatgtagt tcatggccgc
     6301 aatgctggcg cgccccgtat tgcccctttc atcaattacg attttcacat caactccccg
     6361 ttttttggct gctaccagtg ccttcataat atccggggcg gtaaaagaat aagccatcat
     6421 ccgtatcgag gtttttgcgg agtcaatggc gctcaggacg agaacgcggg cgcttccttc
     6481 aggcgagtag ccaacctgaa cggatggttc caccgcaaag gcgggcaggg cggctgtcgt
     6541 aaatgctgcg gccagaagcc aggttgctaa ttttttcatt acaggctccc gttcacacac
     6601 tgcattttat attcagggtc aaaataaatt tctttgattt tccgcttttc catatgcacc
     6661 acaacgtcaa tggtggagta gagcattcgc ataatatcgc tcatatcgag catacggccg
     6721 ataggggtcg ccttgataag cagcccaata cggttaaagg catcgcgcgc agagttagcg
     6781 tgcgttgaca taacaccgcc tggatggccg gtattaagtg ctttaagata atcccacgca
     6841 gcatcatccc taagctcagt catgatgata cggcccggtg tcagacgcat acaggctcgc
     6901 agggcatcag tggcgctgac gcggccgatc tttcctgcat cgccgtacat catataaacg
     6961 gcttctacaa cgtgatcgac cgtgacttcg tgaacgtcct ctaaaataat tacacgctcg
     7021 tctttatgta gcgattttaa cagcgcgcgc gtgagtaccg ttttccccga cccggtttca
     7081 ccgcagatca cgatagtgcg tttcttctca acggcggttt gcaggaatgc gggccatttt
     7141 tcgctgctgt gcagctcttt aaggaaaaaa tcatcatccg ttaggctttg cttgctgccg
     7201 gtaatcttcc ggcagtcact gaaaatcccc tcgctggtca gctgctccag atttttatcg
     7261 gccgccaaat ccttacgaaa cgctacggcc gttgtaccgt caatcaccgc agggggcaga
     7321 cagataacgc ccctgatccc gccaggcagg atcacgtcat taatggcctg catggtcagc
     7381 ttgttgctgc tcaccaacga tttagcaagg ttcctaataa aatctgccgt aattgccgcg
     7441 ttctgcacaa ccctgcggcc gctgaacgta tcacagataa cttcctgaaa gcagttaatg
     7501 cgaatttcaa aaacagtagg atcttctaaa tactcgcgca gtgggccaag ttgatagaaa
     7561 gctgcatcag tcatgattac tcctgaagaa aagcgggcgc taagcgccca cttttttagt
     7621 tgtctgcgag cgtataaacg ccgctgaaat cgaggtcgcg ggcaacaaaa atgctcaccg
     7681 catcaccctg ctgatcgtag agggtagggg ggatagacat gtaagagcgg agtgcttcag
     7741 acgccagctg cccaccgctg ttttctgtgc tgttgtactg aatgttatta ctctgcgtct
     7801 ggttaaccag cgccgttaag gtgtcagaga acaacgaaat catgatcgca ccacgcagac
     7861 gctcccacat atgggcatcc acctggcccg gaatccccgc gctgccgagt gagttcgttc
     7921 cggcactgtc aatattaacg attgtcccgt cctggtcatt acggatacgc tcccagagaa
     7981 caaacacgcg cgcctggccg tctttgatac caccggtaat ctgcccgtca acccatgagc
     8041 ctttatcaat cagcctaacg agtccatcag ctgagtaaac gtcctgtgaa acccggcagg
     8101 aaacctgacc cggaacagtg gtatccagct cggtgccggt accacagggg atcattttgc
     8161 ctttcggaac agtcaggctg ggattagcca tgactccagc gcggctagcc ttcagccttg
     8221 caggagtcag gtttttagcg agtgctgaac taccttcgct tgtttcgttg tcctggggct
     8281 gcgctccggg actattgctt gtagccgcct gattagtctg ggccagctcg ccgcccagac
     8341 gacgctgcat agccagttct tcaggcgaag gttctttacg cttattagag gtacgcgcgc
     8401 cggtattgct gctgcccgca tctgcatctg cctgtgcggc agcctgcaca gcacgggcat
     8461 cagtggcgct attctgcgca gttgcaggtt tattaacatc aggatcgctg ttaaagctgt
     8521 agtttggcag tgtattggcc tgttgcgctt taccaccgtc tttatcagct tcagctttag
     8581 ccggggcgcg aattttaccc atgaccgtaa tcccgatgaa taccaaagca agcagcgcca
     8641 tcagtatgac aaaggctttc ataccaggag ccgaacggcg gttactgcca ttaaatccgc
     8701 cacgctcgct ttcaaattca ccgtctccgg tgttttcatc gagttcctga tctacatcga
     8761 cacttttacg ggccatcagt tatcctcccc aatttgaacc ctgcgcacat ccggggaagc
     8821 cgtaccggtt gctaccgcac cggcgcccgg cgcgaaatta ttattacgaa cgccaacgac
     8881 tttatcgccc agacgaatac gccactcttt agcgacggtt tccacctcga tgatgttgcg
     8941 gttctcaccc acaacatgag agttaggcag cgtttctttg ccactggccg agatcatgta
     9001 gacctgcggt aactccgcat tggccggaaa ctcaaaccgg gtaaagcggt agttatccca
     9061 gacgtgaacc ggctggatgc tgcgcatttc aggctgttcg ctcattacgt actgatagtt
     9121 cttcgccccc gcaaaagccg tctgcttcag cttctgcgta atgcgttttt tatcagccgc
     9181 gcttttggct ttttcctgct gctcaaacgg atattcatag gtcagctgaa gaacggcatg
     9241 gcgcacagcc cacggcgttt caataaagga ttttgatacc gtaccgtctg catttttctt
     9301 cgtttcttca ccgatgaaat ggaggacgat gttataggtg cgcttatcgg tgacgatcac
     9361 caggttggta tcactcatgg cctgtttcgg cttcacaaaa aaatggttca ttttgtgcgc
     9421 aaacgtccag ctttcagaat cgccaaaagc atgagtgata taggtttcgt caggcgcgac
     9481 aacaatgtgg gtagccacac cggcgacagc gtcaattttg accacattaa cagggttata
     9541 aacaacgctt ttaatgcgat agtcataagg agaattgcgg ccaacctcaa gcgccataac
     9601 gttagtggcc gcgcctccca ggactgacaa aacgactgct gaaagaagta gttttttcat
     9661 ggggcagccc tcagttaact tccgggttga cgcgataact cgtcacgcgg aaacccagcg
     9721 ggttgacata acgctgctca gcattcatcg ccagcgattt atattcatac cccataatgg
     9781 caatccagcg ctgcggctga tcatcaacgg gattgctgcg cacgcggcga accgtagtaa
     9841 agcgtattgt tgctacgccg tgcggtttat cgaggatcac agagttaatc ttcacgcggg
     9901 tcgtttcact gtcgcccaga accttatcaa gaccattgcg gcccttgaac ttgctctggt
     9961 aagactctgc cacgttcggc gtggacatta agccaacggc cgtatagtcg acctgaactg
    10021 aatagaagtc atagctctca cggtgaatga catattgtgt cagccagaac ttatcaattt
    10081 cgtcaccata agaggtctgg tcgcgggtca gcttgacctg ctgtacttcg tgagtggcct
    10141 cgttgagcgt tagcagatgt gcggggattg gctggctgta cttatgcact acgtaaccaa
    10201 ctaaagagag tgcaaaaaca gttaccaccg ctgaaccggt ggcaaccatc caggcggtac
    10261 gccgggactt cagcacttca tccatcagat caacttcaag ccctttacgg ctttcgttga
    10321 actctttaat ggcttcacgt gtaagccctg tttttttatt agctttcatt tgcaccacct
    10381 tgcgtatcaa ccgggattgt tttgtttact ggaacggtgt tgctccagtc cggctccggt
    10441 ggcggtttat gcccgctgga acaggcgcta atcagaagaa ctcccataag caataagctg
    10501 cgcattacgg catccattat cggttgttga acgtaccaat tataataagt cgtcgaaatt
    10561 gcaatttcaa cgacttttgt tttaatttta tgcagccttc ttcccgcgac tgccccttgc
    10621 cttaccactt aaattagaac cactgcttcc tccgctgttg ctgccacttc ctccacctgc
    10681 cgattgctgg ccgccgccgg tattaccgcc ccggaacgca ttaccgccaa acatgccatg
    10741 actacccatg ttgccaagcg cctgcattga agaaccggta gaacgtgcag catctgcgac
    10801 accggcactg ataccgctac cccagctctg ggcgacctgc gggatctgaa acagaacaaa
    10861 gatagagata atggtcagca gcagcgcggc catagagcca gtgatagatg aataggccgc
    10921 atcggagttc atcgaggaca gaaggttgtc gaacatctgc atgatgaaac caaacacgag
    10981 agccagcacg acaacgacca gcccgtagtt gattaccgat ccaagccagc gcgcgaagat
    11041 gttttttgtt ggcccccaca gcaggcagaa aatggcaatc gggccgaaac aaagcgtaac
    11101 ggcaagaagg attttcgcca tgatcacaaa gcccgcaccg aggccaccca gcacaacggt
    11161 agcaaccaac ataattccgc caatggcata tgcggccaga ccgcttgacg aaaatacatc
    11221 tgccgcatcc cacgctgtat ttacaatcct gacgcctttt tcaataccgc tatcaatgat
    11281 cgccggtacg ccgctggctt cgactttatc aggggaagat aagatgccag caaaatcatc
    11341 tggcatgtga agcgccacat taaccaaatc ctgctgaaac cagccgcctg ccgtcgcaaa
    11401 gctcaggata agggctatag atagatactc tttaatcagc gagcttaggc tgtcgcctgc
    11461 ccccggatta aacatcgagt acaagccctg catcatcagc tttatggtca ggcaggttgc
    11521 caccagaggc gtaacgtcag agataatggt ggcaacgttg gcgctcacca ttgacgtaat
    11581 cgccccgtct acttttgcga aaatgtcttg taccagggtg aatgccatat tgcctcctta
    11641 ctcttaaatt ttcggtgagt taatttcacc gtcctcttta ccgtgaacaa acagctggcg
    11701 atcagcctca cgcgcgtttt tgcagtctgg cttctggagt tcatcagcat tcttcttgca
    11761 ctcctcgatc gttgccttgc gctctttgtc atgctttttg taccaatcaa cgtcatgcga
    11821 agcgtcgcag gccaccagga agaaagggat aagcagcagt agttttttca taatttcctc
    11881 agttaattga aggtgacgcg gtaacgtccc caccggttcc aaaaacaaaa ttacgggtcg
    11941 cacgatcctt ctgtgcgcgc aaaagtttgt cctgcgattg ctgtaacata ttcatcaaat
    12001 tcagcttcgc ctgctcaccc tgtatagcgc cttgtgacgt ctggatacgt gcttgcaggt
    12061 cggcaatgga tttcaggtct ggcgtcgatt taatctgctc cgtcagcgcc tgcatatcgc
    12121 tcagctcttg catctggttg ttgtaggctt tttcggccat cacacggtcg taagcacctt
    12181 tttccgccag ctttttgttc atataggcga ttgcttcgct gggcgtcatg tcgtcaacct
    12241 cagcattaaa ctggcccatc atgctgttaa cagaaggcgt gaccgacgaa ctggagttca
    12301 tcgcgtcgct gtacacctct ttccagttgt caggcaggtt attcgccagc gtattggttg
    12361 acgtccccag cagatcgccc aggttggttg ttttagccat cgactcatac atatttttct
    12421 gcgtctccag ctggctttta agctgctcca gctgttgagc catttgttca agctgttcac
    12481 cctgtttaat cagctcggtg gggttggata cgatgatgcc agcggaggcg ctttgcgcgc
    12541 ccccaagaat caagccggtg gtcagcagca ctgccgtaag tgattttttc atggagtttt
    12601 gcctcgttgt taagccgtca ggcgccagta ttctttgagc catacttcag ggtcattacc
    12661 gagccgttca accagctcat gcgcaatttc ggcgttttgt ggttcaccgg acaacacgct
    12721 aagtacgttg tccattgtct taatatctgc atcaatatcg tcgctgttgc gagggtagag
    12781 attgaaagaa gcaatcgcag actgttgccc ttgtttaacc aggaactgtc tggagtgttc
    12841 ggtaatcgac atcagcgcgt cgtattcagc atcagtaagg aaggcataat cctctcggat
    12901 agcttccgga tcgcgcaggc agatttttgt gacggtctgc gacatgatcg tgcggccaat
    12961 acggctggac agcgcatcgt tcggctcctg cgtggcaaac acatagatag cgtctttctt
    13021 acggtcagtt ttgataccac gcttaacttc acgctcgata accggatcgt caaggtaggc
    13081 gtggaactcg tcaaagcact gaatgacgcg gcgtttgccg tcgatggagt cacgtacccg
    13141 gtacagaagg tacatcatga gcggagtacg ggccgggctg gatacttcct ctttggctgc
    13201 gataaactcg gataaatcga agccaaaaat atcattcgcg ctgagatcca ggctgtcttt
    13261 atcattgtca aacagccagc catactggcc ttcgcgcgtc cattcgcgca gcagcccttt
    13321 cagtgatacg ccattttcca caacttcatt cacgtacccg tccaggatcg taacggtgcg
    13381 cgcctcgcgt ggaataagtg agccttcccc cataaccgca tcaacgcctt cagccagttc
    13441 ggtagccatc gttgcgctga taggcccgtt attggtggtt tccacacaaa tgcggaacag
    13501 gtttttaatg agggcaatat tgcgtttggt cggttcaatc tgaagcgggg caaacccgga
    13561 cggcataccc tgttgcagaa ctttatagta gccaccaacg cttcgaatga acggctccat
    13621 accgcggtca cggtcataaa caaaaagtcg cgggttgtac ttcattgact gcgccagcag
    13681 gaagttaagc agcgtggttt taccttcccc cgacataccc gttattaacg catggcccag
    13741 tgggcgttta ccgtaggaaa gttcttcaag cggggtcaca tggaaattaa aatagagtgg
    13801 cgtaccgctg atcgtgcgga acatggtcag cgctggcccc cacgggttat tgtcaggctt
    13861 gccacgcata aaattgtgga acgggctgaa gtgcaggaag ttccatgagt ttatcggaac
    13921 cgggcgcggc gcccatttct ggttgccagg cagtctcgca taatatgcag cctcagaggc
    13981 caggctgaga gtcccaccaa ccacgccaca gccggttagc ataaccttca cacgacgcgc
    14041 tttgcgctgt accgcgtttt ggtcattatc ccagacatgc acggttccat gatggtagcc
    14101 catcacgaac tctctggacg tcagcatatc gagcgcggta ccaagctgtg ccagctggct
    14161 ttgcgcacgg tcgcgcgttt cctgcaaaga tttttcctga tgcgtcagaa acgttttagc
    14221 tgaagattca gagaggcaag aaaaactctg cgtcagaagg tattcaaaat cggcttcttt
    14281 aagcatgtta agctggcctg gctctgtatc ttcttcgtat tcacgaaatt caatgccggt
    14341 ggtatagaag ttgtgatcta ccgttctgat ctggacaaca tccccccaca gggagctaac
    14401 agggcggttg tccatgatgt actcacggat acgatcacgg caaacaggca cgatggccca
    14461 ttccatattt gcgaggaaat aaaggaactc cagcgctttt gaataagcgt gagcctgcga
    14521 aggttcaggc tcgttgcgtt cgataacaat ggcttcgtca aaaatgtctg attcatcgac
    14581 ttcagcaagt tcttcacgtt cttttttatc aggcgcagga atttcaacac cgcgtttgtc
    14641 acgataatag atacccaact gctgaatgcc atacggcttc attgcttcca ggatttgttc
    14701 agaaatatct tccagacctt caagcgcctc attctgcatt tgctgaattt cgtcacgagt
    14761 cggcttttca aatttcgcca gaaacttctg tgttttatcc cctacctgtt tgtaaataac
    14821 ggtcagataa aggtcattaa tcagctgctt ggaatcaccg tgcaacttac ggttatattg
    14881 atcaatataa gcagggaaaa aatggtcata ctcaccatcc gggtactctt tagcctcatg
    14941 gtgatattca tgcgtccaca gctctacatg gtctgttccg aagctcttga ccagcgtatt
    15001 aaggtcttta tgccaggtga ccagttcccg atctgatgcg cagtcatgtg tgcggccatc
    15061 cagcttgaaa aaagccatca gatcgccgtt ttccatcgaa atcacgtaat cattgaggtg
    15121 ataagaatag ggcaaatact ttttatttac tgatggctcc ttacggtagg catcaatttt
    15181 ttttggcttc gtagcggtgg cagctctcat tattttaaac ccttacgttt gtagtcaaca
    15241 gaggaataag acgatcctcc ccactgctta aacggagaat caaaccaatt actgaatttg
    15301 gttttcaacc acaggcccat aattcgaaac atgcggtcgt catattttgt taatgcagct
    15361 gaaggaatcc acaaaaagac gaacaccagg atcgcccaca tatgaataat cataaaaagc
    15421 gaggcagaaa tcatgaatat catcatagct acattacgtg gtacacccag cgcgcgaggt
    15481 aagcgagtcg cacctttgaa aagcggtctt ttcccgtcaa cgaacatctt tgtagtcctc
    15541 actttggggg acaaaagtcc ccccttttcg cgttgttcag cttcctacgc ccgtcaggga
    15601 aaccagatat gatgcagaac caataccaat cagagagatt acgatacgag gaataaagct
    15661 ggctgggatt acgtgaagca tccacataaa gcaactaacc atgatcgcaa tagcacaacc
    15721 aattggaatc catgtgctca accacgtctg gattgatgta gcggttgatt caccagtatc
    15781 ggtgccagcg gccagcgcaa tttgcggcag ggcaatggac aaaacgccca taactaccgc
    15841 agggccatac ttcttaaaca acgtggtcat actttctcct tactcactat cttctgttga
    15901 ttgcagattt aaccggcatt cttcaacgcc acacttatct ttcaaaaact ctgccagtag
    15961 ccttaaatca gcccaggttc gtagctcatg ctctttgtta acccgaatgg aacatgcgat
    16021 ctggcccagc tgttcatcgt ctatctgaaa aacgggatac cacttcttta atgaaacccg
    16081 ttctatcgtt acctccttca cttttcctgc ttcaaataac tccaggatct gtgattgcaa
    16141 tacaagatac aaacgttcca ttcataacct cattccccct tcgctgtttc ctgtctggtt
    16201 aaaaaggcat ccgtattatt tcggctgaag gcatcgccat caccagaacc aaaaacatct
    16261 tgttcaccgt catactgcgg cgccgtactt tttgcctgct gaggctcatt cgcggtgctg
    16321 tcctcactgg tctggccgtc aggtagcagc gtagggattt tcaaatcagt tgattgacgc
    16381 gccacgttga taactttcgt gacatacccg ttagaaatcc cgtttgtgag tgagccggtg
    16441 ttgtagcagg aaagcgcgtg tctcagcgca acctgcccgg ctgggtagga tttcagggcg
    16501 ctatcataac aggctttaag aatggtctgg ctcgcccgca ggttgatgca gggtttgaaa
    16561 atatcgtcaa ccgaaagacc caggcccact aaattatttg agttaatttg tgcaaggccc
    16621 atatcaaaac ttttattatc cttcagcaga actttcgcaa cgctgacggc ctcagcttca
    16681 gtacgcggtt gctgttttaa ctgaatacta ccgttaatat tgatcctgta cggtccattt
    16741 gaggactcat ggccgacgat gtacgccatc gttaagggtg aaacatcagg cgcacatctc
    16801 tgcgcaagcc tggcaacttc atcggaggca ggcgcagcac tggcacggcc agcacaagca
    16861 aggcaggcca gagcgagaac caggagtttt ggatgtttac tcatttatgt ttcaccgcgt
    16921 aattgtaatt tcgacgactt ttgtttatga taagggctaa tgagatcgtg tcaagaagaa
    16981 acgaaaagaa aaccgctgaa ggaaggaagg tgatgctggt tcccctgaaa tcagaaaaac
    17041 gaccaaaagg cgaacccgtg tatcgtgacc cggataaccc ttttaatacg tggactggta
    17101 tagggaagcg cccggcctgg ctaactgcaa aattggacgc tggcattagc ctggaagcca
    17161 tgaagatgca gggcgtagcc aaccccagag aacatagacc agcaaaatac cgcgacccca
    17221 ggaacgcaga aaatacctgg tccgggactg gccgccgacc cacatggctc aaagagctgc
    17281 ttgatagtgg tttatcactt gatgatctga agatataacc ggagggtata aaaatgggcg
    17341 aacttattga ttttgcggaa aggcaaaaaa gcagaaggaa gaaaaaagca tcttccattc
    17401 cgcccatttt tagaaaattt cgtgtccatg cgatcaggtt actcgccagc atcatcaaat
    17461 ccggctctta ttcagttgct tatatcgtta aaaaaattac aggaaagtta atcaagtttt
    17521 acacaatttt aacgattttc attttt
//
LOCUS       DAPHBJ010000090        17448 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.091, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000090 DAPHBJ010000000
VERSION     DAPHBJ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.091"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(150..956)
                     /gene="trpA"
                     /locus_tag="RHZ76_002634"
     CDS             complement(150..956)
                     /gene="trpA"
                     /locus_tag="RHZ76_002634"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDV2813250.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(956..2149)
                     /gene="trpB"
                     /locus_tag="RHZ76_002635"
     CDS             complement(956..2149)
                     /gene="trpB"
                     /locus_tag="RHZ76_002635"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDV2813251.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2161..3522)
                     /gene="trpCF"
                     /locus_tag="RHZ76_002636"
     CDS             complement(2161..3522)
                     /gene="trpCF"
                     /locus_tag="RHZ76_002636"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415778.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HDV2813252.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEEQERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3523..5118)
                     /gene="trpD"
                     /locus_tag="RHZ76_002637"
     CDS             complement(3523..5118)
                     /gene="trpD"
                     /locus_tag="RHZ76_002637"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HDV2813253.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5118..6680)
                     /gene="trpE"
                     /locus_tag="RHZ76_002638"
     CDS             complement(5118..6680)
                     /gene="trpE"
                     /locus_tag="RHZ76_002638"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDV2813254.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCEDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQSLSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENSIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6702..6795)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6772..6816)
                     /gene="trpL"
                     /locus_tag="RHZ76_002639"
     CDS             complement(6772..6816)
                     /gene="trpL"
                     /locus_tag="RHZ76_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HDV2813255.1"
                     /translation="MKAIFVLKAWWRTS"
     gene            6954..7835
                     /gene="yciV"
                     /locus_tag="RHZ76_002640"
     CDS             6954..7835
                     /gene="yciV"
                     /locus_tag="RHZ76_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HDV2813256.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7832..8452
                     /locus_tag="RHZ76_002641"
     CDS             7832..8452
                     /locus_tag="RHZ76_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDV2813257.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8480..10375
                     /locus_tag="RHZ76_002642"
     CDS             8480..10375
                     /locus_tag="RHZ76_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HDV2813258.1"
                     /translation="MAGKFRCILLLIAGLFVSSLSYAENTEIPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMIIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRIFEDGRIESSDPFYREDFTVL
                     YRWPHALLSNAPAPQTTNIFSHILLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQYDKNQQILICV
                     NEGATRNNEVLLPAEQLLLEALFRKGDKVVLTGRRNRLLRRAFLRMQKFYLPRKKSSF
                     YRPDTFLQWGGLAILAVILYGNLSPVGWAGMSLVGDMFIMICWIIPFLFCSLELLFAR
                     DDDKPCVNRAIITLYLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVCLTGYLTGM
                     GYIFLPKFTQTGQQRYARGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAISAN
                     LGREWALRIAPSLSSAIRAPEIARNGVLFSLQTHLSCGANTSLLGRSYSGGGAGGGAG
                     GGGVGGW"
     gene            10586..11461
                     /gene="rluB"
                     /locus_tag="RHZ76_002643"
     CDS             10586..11461
                     /gene="rluB"
                     /locus_tag="RHZ76_002643"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HDV2813259.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNNG"
     gene            complement(11501..12091)
                     /gene="cobO"
                     /locus_tag="RHZ76_002644"
     CDS             complement(11501..12091)
                     /gene="cobO"
                     /locus_tag="RHZ76_002644"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HDV2813260.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(12088..12846)
                     /locus_tag="RHZ76_002645"
     CDS             complement(12088..12846)
                     /locus_tag="RHZ76_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HDV2813261.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCRSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            13066..14115
                     /gene="sohB"
                     /locus_tag="RHZ76_002646"
     CDS             13066..14115
                     /gene="sohB"
                     /locus_tag="RHZ76_002646"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HDV2813262.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRLTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(14151..14402)
                     /locus_tag="RHZ76_002647"
     CDS             complement(14151..14402)
                     /locus_tag="RHZ76_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HDV2813263.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            14782..17379
                     /gene="topA"
                     /locus_tag="RHZ76_002648"
     CDS             14782..17379
                     /gene="topA"
                     /locus_tag="RHZ76_002648"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDV2813264.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         4241 a         4344 c         4663 g         4200 t
ORIGIN      
        1 atattactgt tgggcggaaa aatgacgtaa gttgacgttc gaccggggta agcgaaacgg
       61 taaaaagata aatattaaat gaatttagga tttttccggc ttcattaaag aaagttaaaa
      121 tgccgccagc ggaactggcg gctgtgggat taactgcgcg tcgccgcttt catcggttgt
      181 acaaaaactt tcagtgccgc cagcattttc tctggctcat taatatgttg ctcgatgatt
      241 ttaacaatgg ccgaaccaga aatcgcgccc gcagctcctg catcaatcgc tgcttttacc
      301 tgatccgggg cggaaatacc aaatccctgc aatggaggtg cagcgttgta ctctttcagc
      361 ttcgcaacca gatgattgag gggtaacgcg gcgcggtttt ctgcgccggt cacgcctgct
      421 cgtgacagca aataggtgta accacgaccg taagaggcta tctggcgcag caggtcgtca
      481 tcggcatttg gcgggcagat gaagataggt gcgacattat gacgcaacgc ggcctggcgg
      541 aagggcgcgg actcttcaac tggcacatcg gcaaccagca ccgaatcgac gccgactttt
      601 tcgcactggg cataaaactc atcaatgcct ttgttaaaca ccagattggc atacatcaac
      661 aggccaatgg gaatggtcgg gtgtttctgg cgaatcagtg ccagcatttc aaaacattgt
      721 gccggagtca cacctgccgc aaaggcgcgc agagtggcgt tttgaatcgt cgggccatcc
      781 gccagtgggt cggagaaggg gatacctaac tccagcgcgt cagcaccggc ttcaattagc
      841 gtatcgataa ttttcaatga ctgctcaatg cccggatcac cgagcgtgac gaaaggaacg
      901 aatgcgcctt ctttgcgctc cttcaactgg gcaaacagag attcgtagcg ttccatcaga
      961 tttcccctcg tgctttcaaa atatcgtgaa cggtgaagat gtctttatcg ccgcgaccgg
     1021 aaaggttaac caccagtagc tgctctttat ccgggttttc gcgcatcatt ttcaacgcat
     1081 gggccagggc gtgggaggat tccagcgccg ggatgatccc ttcgtgcagg cacagcgttt
     1141 tgaaggcttc aagggcttca tcatcggtaa tagacacgta atcagcgcgt ccagtgctgt
     1201 taagatacgc gtgttgtggg ccgacagacg ggaaatccag tccggcggag atggagtaag
     1261 attcttcaat ctgcccgtct tcggtttgca tcatcggcgc tttcataccg aaatagatac
     1321 ccacgcgacc atgttttagc ggtgcgccgt gctcgccagt ttcgataccg tgaccacctg
     1381 gctccacacc aatcaggccg acgttggttt cattgatgaa atcagcaaac atgccgatgg
     1441 cattcgaacc gccgccaaca caggcgataa cggcatccgg caggcgacct tctctttcca
     1501 gaatctgcgc tttggtttct tcgccaatca tccgctgaaa ctcacgcaca atggtcggat
     1561 aaggatgcgg gccagctgcg gtgcccagca tatagtgcgc ggtttcgtaa ctaccggacc
     1621 agtcgcgcag cgcctcgtta caggcatctt tcagcgtcgc ggaaccgcta tgcaccggga
     1681 tcacttccgc acccattaag cgcatacgaa aaacgttagg cgactggcgt tcaacgtctt
     1741 tggcacccat ataaatacgg catttcaggc cgagcagggc gctggcaagg gccgacgcca
     1801 cgccatgctg accggcaccg gtttcggcga tgatttcggt tttacccatc cgcttcgcca
     1861 gcaacgcctg ccccagcacc tggttagttt tatgcgcgcc gccgtgcagc aaatcttcac
     1921 gcttgagata cagcgtggtg ttcgtcccgg ctgtaatgtt ctggcatttg gtcagcgcgg
     1981 ttggacgccc ggcatagttt ttcagcaggt cgttgaactg agcctgaaat tcaggatctt
     2041 tttgcgcact gacaaaagct tcttccagct ggcgcagagc aggcatcagg atttgtggca
     2101 cgtacatgcc gccaaactca ccaaaatagg ggttaagtaa tgttgtcatt gttcctttcc
     2161 ttaatatgcg cgcagcgtct ggaaaaccga ggccaaaaga cgtgcgtctt tgatgcccgg
     2221 ttgcgactct acagcagaat taaaatcaag tccggcgcag ccggtttgtg ccgcttccac
     2281 gcagttatct gcgcctaagc cccccgccag cagaacgttg ccaagcgatt gaccatttaa
     2341 tagtgaccag tcaaaacgtt gcccgcttcc accctggccg ttgtctaaaa catatttatc
     2401 aacgtgctga aactcgcggg cgggcagggt ttcaccgacg cttaatgctt tccagatggc
     2461 aacatgtgct ggcagagctt cacgcagcgt atcgatatac agctgttctt cattaccatg
     2521 cagttgcact gccgccagcg ataacacctt agctttgtcc accacatcgg caatatcgtg
     2581 attgcggaac acgccaacat actgcaacgg tgccgcagcc atcacttcct gcgcctgttc
     2641 aacgttgacg caacgcggtg atgtcgcaac aaaaatcaac ccaccgtaaa tcgcgcccgc
     2701 gtcataagct gctttagcat cttgcccacg cgtcaggcca catactttat tctcacccag
     2761 caacacccgg cgcacggcgg cgtgcaaatc gtcatgggcc atcaacgccg aaccaatcag
     2821 aaaaccgtta gcgaagtggc ttaactcgcg cacctgagcg taagtattga tgccggattc
     2881 gctgattacc gtcacgttgt gccccagttt cggcgcaagc tcgcgggtac ggttgagatc
     2941 aatcgacaaa tcacgcagat cgcggttgtt gatgccaacg acctttgctc ccaatgcaat
     3001 ggcgcgctcc tgttcctctt cattactgac ttcggtcagc acccccatct ccagactgtg
     3061 agcgacggcg gcaagctggc gatattggtc gtcatccagt actgaaagca ttaataagca
     3121 ggcatcggcc tggtaatagc gcgccagata gatctggtaa gggtcgataa tgaagtcttt
     3181 acataaaatc ggctgcgggg cgatttggct gacgatgggg aggaaatcaa agctcccctg
     3241 aaaatatttc tcatcagtca gcaccgaaat tgccgaagcg taatgtttat aaatggcggc
     3301 aatgcgtgct ggatcgaaat catcacggat cacgcctttt gacggcgacg ctttcttgca
     3361 ctccagaata aacgccgtgc gcgcaccctg tagcgcatca taaaaatgtc gcgtgctcgg
     3421 ctgaacctca ttctgaaaac tggccagcgg ttgctgctgt ttgcgggctt ctacccaaat
     3481 cgccttgtct gcgacgattt tcgctaaaac ggtttgcatc atttaccctc gtgccgccag
     3541 tgcggtgact ctgtcgtaag cggaaccact gcgcagtacc tcaagaacgg tttgcgcatt
     3601 ggcttgcaga tcttcatggc catgcaggcg cattaacatg gcgacgttcg cagcgacggc
     3661 tgcttcatgg gcggcgtcgc ctttaccttg taacaaacgt gttaaaatgt cacggttttc
     3721 ttccggtgtt ccgcctgcca gttgctcctg gtggtagggt gtcaggccaa agtcttctgc
     3781 ggtgagctga tagcttttaa tttcgccgtc atgcagttcg gcaacgattg tcggcgcgtg
     3841 taatgaaact tcatccatcc cgccgctgtg caccaccgcc gcgcgttgat accccagcac
     3901 gcgcaaggtt tcggcaatcg gcagcaccag ttccggacta taaacaccaa ttaacgccag
     3961 cggcggatgc gccgggttaa tcaatggccc cagcacattg aacagggtgc gggttttcag
     4021 ttgctggcga accggcatcg cgtggcggaa tccggtgtga tacttcggcg caaagaggaa
     4081 acatacacct aactcatcca gcgcctggcg cgatttatcg gcgttcatat caagattaat
     4141 accgaacgcc gccagcagat cggacgaacc agatttactg gagacgctac ggttgccgtg
     4201 tttcgccact ttcagcccac aggccgcggc gacaaacgca ctggcggtag aaatattgat
     4261 actgttgctg ccgtcaccgc cagtaccgac gatatcagca aacagataat ccgggcgcgg
     4321 gaacggcgct gcgttttcca gtagcgcggt tgctgccccg gcgatctcgt tcgggtgctc
     4381 accgcgaatt ttcatgctca ccagcgccgc cgccagttgt tccggcttca gctcgccacg
     4441 caccaccgct gaaaacagct ggtggctttc ttgttggcta agcgtctgcg cctgatacag
     4501 tttttccaga atcggttgca gcgtgttggc tggctctagt ttctgctgcg cccaggccag
     4561 cgtttgttcc agcaggcgag cgccctgggt ggtgagaatg gattccggat ggaactggaa
     4621 tccacaaacg cgatccgcat cgtgacgtac tgccatcacc atgccattaa aatgggcgtt
     4681 gatggttaaa ccggccggaa tgttactgcc aaccagcgag tgataacgcg ccaccggcag
     4741 cgggtttgtt aatccggcaa acatcgcctg accgtcatgt tcaatgctgg aggctttacc
     4801 gtggagaatt tcgcccgcct gaccgacata gcccccgtaa gcttcgacaa tcgcctgatg
     4861 tccgaggcaa atgccaataa tgggcagctt gccacgcaag cgggtgagga gttccggcat
     4921 acaaccggct tcgctcggca caccggggcc aggagaaagc atcagcaccg gattgctcat
     4981 ggtcgccagg cgttcaatta aggtttgcgc cggaatatgg ttgcggtaaa tcaccacgtt
     5041 atgcccattg ctgcgcaact gatctgccag gttgtacgta aaagagtcga tattatcgag
     5101 cagcagaatg tcagccatca gaaagtctcc tgtgcatgat gcgcggtggc aatagcgcgc
     5161 agtacagcgc gggctttatt acgggtttcg tcggcttccg actgcggaac agaatcaagg
     5221 actacgccag caccggcttg cacggtggcg atactgtttt ccaccagcgc cgagcggatc
     5281 acaatgcagg tgtcgagatc gccatgcgcg gtgaaataac ctaccgcgcc gccgtagctg
     5341 ccgcggcgac gaccttcagc ctcggcaatt aactgcatag cgcgtacttt cggcgcaccg
     5401 cttaacgttc ccatattcat acaggcgcgg taagcgtgca gggcgtcgag atcgtggcgc
     5461 agctcaccga ccacgcggga gaccaggtgc atcacgtaag agtaacggtc aactttggta
     5521 agatcggcga cgtagcggct gccgggggtg caaatgcgtg ccagatcatt acgggcgaga
     5581 tccaccagca tcagatgttc agaaagctct ttatgatcgg tacgcatttc cagttcgatg
     5641 cggctgtcga ggtctctgtc cagtgaacca tcggcacgac gaccgcgtgg gcgtgtcccg
     5701 gcaatcgggt agatctcaat ctggcggctg gtggcgtcat atttgagcga actttccggc
     5761 gacgcgccaa acagggtgaa atcattatcc tgcataaaaa acatgtacgg gctgggatta
     5821 ctctttttca gcacgtaata ggccgccagc ggtgacgggc agggcagaga gaaacggcga
     5881 gatggcacca cctggaaaat ttctccagcg cgaatcgctt tttgcaacaa acgcactacg
     5941 ccaccgaact cttcatcgct ctggttacat tcacaacgca tatgcggcac ggaaaccacc
     6001 ggcagcggtg gcgcggtttc ggtcagttgc tggcgaagat cgttcaggcg agcagtgaga
     6061 cgttgttttt cttcttcatt cggagcaaac aggctggcct gaatgcgggt gctttttttc
     6121 tgatggtcaa tcaccatcag cgtttcagcg agataaaaac agaaatcagg gcagttattt
     6181 tccgctgaca gttgcggtaa atcttcaaac cccgccacaa ggtcataaga gaacaggccg
     6241 ccgaagaaca tggcttctcg ttcttccttc ggtacattca acagattctg caataagcgg
     6301 aaagcgtcaa aaaccgaaag ggagcataag cgagcgtctt catccagcag tggactaaca
     6361 ggggggaagc gcagcacacg gcagtttggt aattgttcat tttccacacc cgcaggcagg
     6421 gcgttatcca gtagtgtcag cagggcttcg ccgttgccgg aaagggactg gattgtgaca
     6481 gtgtcaccta aagctgtaat gcgtagcgca ctgtctacca gcagcaggct ttttaaatca
     6541 tctttgctgt cgatatctgc ggattccagc agcagcgttg ccggacgatc ctcacacaat
     6601 tggtgaaaaa gcgcggtggg attgtcgcga taagcgcctt cgcaggttag cagttcgaga
     6661 gtcggttttt gtgtttgcat tgttattctc taattttgtt caaaaaaaag cccgctcatt
     6721 aggcgggctg ggtatctgat tgctttacgc atggtgaata cactgcccgt ttcaggaagt
     6781 gcgccaccaa gctttcagta cgaaaattgc tttcattgtc gatacccttt ttacgtgaac
     6841 ttgcgtacta gttaactagt tcaatgatta attgtcaaca gctcatttca gaatatttgc
     6901 cagaaccgtt atgatgtcgg cgcaaaaaac attatccaga acgggagtgc gccttgagcg
     6961 acacgaatta cgcagtgatt tacgacctgc acagccatac cacagcttcc gatggctgcc
     7021 tgacgccaga agcattggtg caccgtgcag tcgagatgcg cgtcggcacc ctggcgatca
     7081 ccgaccatga caccacagct gcaatcgcgc cagcaagaga ggaaatttca cgttcgggac
     7141 tggcgctgaa tcttattccc ggcgtggaaa tttccacggt ctgggaaaat catgaaattc
     7201 atattgtcgg gctgaatatt gatattactc atccgctaat gtgtgagttc ctcgcgcagc
     7261 agacagaacg gcgcaatcag cgggcgcagc tgattgccga aagacttgaa aaagcgcaaa
     7321 tacctggggc gctggaaggc gcacaacgac tggcgcaggg cggcgcagtg acgcgcggtc
     7381 attttgcgcg tttccttgtt gagtgcggca aagccagttc aatggcggat gtctttaaaa
     7441 agtatctggc gcgcgggaaa accggatacg ttccgccaca gtggtgtaca atagaacaag
     7501 ctattgatgt cattcatcat tctggcggta aggcggtgct agctcatcct gggcggtaca
     7561 atcttagtgc taaatggctg aaaagattgg ttgcgcattt tgccgaacac cacggtgacg
     7621 cgatggaagt cgcgcagtgc cagcaatcgc ccaatgaacg tacccagctg gcggcccttg
     7681 cgcgtcagca tcatttatgg gcatcacaag gatctgattt tcatcagcca tgcccgtgga
     7741 tcgaactggg tcgtaaactc tggctgcccg caggcgttga aggcgtctgg cagctatggg
     7801 aacagccgca gaacaccaca gagagggaat tatgagccag tttttttata ttcatcctga
     7861 taacccacag caacgtctga tcaaccaggc ggtggagatc gtgcgtaaag gcggggtgat
     7921 tgtttatcca actgattccg gctatgcgct cggctgtaaa attgaagata aaaacgcgat
     7981 ggagcgtatt tgtcgtattc gccagctgcc ggacggtcac aactttaccc tgatgtgtcg
     8041 cgatctttct gaactctcga cctattcatt tgtggataac gttgcgtttc gtttaatgaa
     8101 aaacaacacg ccgggcaact ataccttcat cctgaagggg acgaaggaag tgccacgccg
     8161 tctgttgcag gaaaaacgca aaaccatcgg tatgcgtgtg ccttctaacc cgattgcgca
     8221 ggcgttgctt gaagcactgg gcgaaccgat gctttccact tcgctaatgc tgccaggcag
     8281 cgaatttacc gaatccgatc ccgaagaaat taaagaccgt ctggaaaaac aggtagattt
     8341 gattattcac ggcggctatc tcgggcagaa accgacaacg gttattgatc ttaccgatga
     8401 tacgccggtc gtggtgcgtg aaggcgtagg tgatgtgaag cctttcttat aacaaattgc
     8461 gttccacgga tggaagacta tggcagggaa gtttcgctgc attttgctgt tgatagcagg
     8521 gctgtttgta tcatctctaa gttatgcaga aaacacggag atcccttctt atgaagaagg
     8581 gatttcgctc tttgatgttg aagccactct gcaaccggat ggggtgctcg acatcaaaga
     8641 aaatattcat tttcaggcgc gaaatcagca gattaaacac ggattttatc gtgatttacc
     8701 acgactctgg atgcagcctg atggggacgc tgcactgctg aactatcata ttgttggcgt
     8761 cacccgtgat ggtattcctg aaccctggca tcttgactgg catatcgggt taatgattat
     8821 tgtcgtgggc gataaacaac gtttcttgcc tcaaggcgac tatcattatc aaattcatta
     8881 tcaggttaaa aatgctttcc tgcgtgaggg agattcagat ctgttaatct ggaacgtgac
     8941 tggtaaccac tggccgtttg aaatctataa gacccgattt tcactcaagt tccctgatat
     9001 cgcgggtaat ccatttagcg aaatcgatct ctttactgga gaagagggcg acacatatcg
     9061 aaatggccgc atctttgagg acggaagaat tgaatccagc gatccgtttt atcgtgaaga
     9121 tttcacggta ctctaccgct ggcctcacgc tttactcagc aatgccccgg ctccacaaac
     9181 gacgaatatt ttcagccata ttcttttacc ctccacgtca tcgttgttaa tttggtttcc
     9241 gtgtctattc ctggtttgtg gatggttata tctctggaag cgcaggccgc aatttacgcc
     9301 ggtagatgtg attgaaaccg atgtcattcc gccagattac acgcccggca tgttacgtct
     9361 cgatgcgaag ctggtttacg acgataaagg tttctgtgcc gatatcgtaa atctgatcgt
     9421 gaagggaaaa attcatctgg aagatcagta tgataagaac cagcaaatcc tgatttgtgt
     9481 taatgaaggc gcgaccagaa ataatgaggt attactgccc gcagagcagt tattactgga
     9541 agcgttattt cgtaaaggcg ataaggtcgt tcttacgggg agacgcaaca gactcttacg
     9601 cagggcattt ttacggatgc agaaatttta tctgccgcgt aaaaagtctt cgttttaccg
     9661 gcctgatacg tttttgcaat ggggtggact ggcaatattg gcggtcattc tctacggtaa
     9721 cctgagtccc gtaggttggg caggaatgag tctggtaggc gatatgttta ttatgatctg
     9781 ctggattatt ccttttttat tttgttccct tgagctttta tttgcccgcg atgatgacaa
     9841 gccttgcgtt aatcgtgcaa tcatcacttt gtatttacca ctgatttgtt caggcgtggc
     9901 cttctattct ctctatatca atgtcggaga tgtattcttt tactggtata tgccagcggg
     9961 ttattttagc gctgtttgcc tgaccggtta tctcactggc atggggtata tttttctgcc
    10021 aaagtttacc caaactgggc agcaacgtta tgcccgcggt gaagctatcg ttaactatct
    10081 tgcgcgtaaa gaggcagcaa cacacagtgg acgtcggcgg aaaggggaaa cacggaaact
    10141 ggattacgcg ttgctaggtt gggctatatc ggcaaatttg gggagggaat gggcgttacg
    10201 cattgcccct tcgctttctt cggcgattcg cgctccagag attgcccgta acggcgtttt
    10261 attctcatta cagacgcacc taagttgcgg ggccaatacc agtttgttgg ggcgaagtta
    10321 ttccggtggt ggtgctggcg gcggcgcggg tggcggaggc gttggtggct ggtaattaag
    10381 ctgatgttaa tcgaaacaag ctttaaccat aaagatagtg tagtatgttg cgcctcaaag
    10441 ccaggccgat aaacgtcgag tcgtttactt aaggcctgaa gagttcaaac agactttact
    10501 atcagtgagt cagatgtaag ttctgactca ctgttgcaca aaagcatatc agacgcctgg
    10561 gaaggcgaca ccaaaaggaa gctctatgag cgaaaagcta cagaaagtgc tggcgcgtgc
    10621 cggccacggt tctcgccgtg aaatcgaatc tattattgaa gccggtcgtg tgagtgttga
    10681 tggcaaaatt gctaaactcg gcgatcgtgt tgaagttacc cctggattga aaatccgtat
    10741 cgatggtcac ctgatttcgg tacgtgagtc cgctgaacaa atttgtcgcg ttctggccta
    10801 ttacaaaccg gaaggtgagt tgtgtacccg taacgatccg gaaggacgtc caacggtgtt
    10861 tgaccgtctg ccaaaactgc gtggcgcacg ctggattgcc gtgggtcgct tggacgttaa
    10921 tacctgtggt ctgctgctgt tcaccaccga tggtgaactg gcaaaccgtt taatgcaccc
    10981 aagccgtgaa gttgaacgtg aatatgccgt gcgtgtattt ggtcaggttg acgacgcgaa
    11041 actgcgtgat ttaagtcgtg gcgtgcagtt ggaagatggt ccggcagctt ttaaaaccat
    11101 caagttcagc ggcggcgaag ggatcaacca gtggtacaac gtgactctga ccgaaggccg
    11161 taaccgtgaa gttcgtcgtc tgtgggaagc ggttggtgtg caggtgagcc gcctgatccg
    11221 tgttcgttac ggtgatatcc cactgccaaa aggtctgcca cgcggtggct ggacggagct
    11281 ggacctcgcc cagactaact atctgcgcga actggtggag ctaccgccag aaaccagctc
    11341 taaagtcgct gtagaaaaag accgtcgtcg catgaaggcg aatcagattc gtcgtgcggt
    11401 gaaacgtcac agtcaggtga gcggcggtcg tcgttctggc ggacgtaata ataacggtta
    11461 atcaaagtat gccggacgtc atatccggca tttttacaga ttaataatcg atccctatct
    11521 gcgcttttac accggcatca aacgcatgtt tgacggggcg taattcactt accgtatctg
    11581 ccagttcaag aatatcccga tgacaaccac gacccgtgat aatcaccgtc tgttgatgtg
    11641 gacgttcatt taacgcctgc accacttctt ccagtggcaa atagtcatac gccaccatat
    11701 acgtcagttc atcaagcaaa accatatcca gtgaggaatc agcaagcatc cgctttgcat
    11761 gttgccagac ttcgcggcag gcggcggtat cagactcgcg gttttgtgta tcccaggtaa
    11821 agcccgttgc catcacctga aactcaacgc catgtggctc cagcagattg cgttcgccat
    11881 taggccaggt gcctttaata aactgcacga cgcctacttt ttttccgtga ccaactgcgc
    11941 gtgttgccgt accaaatgcc gcggtggttt tgccttttcc attgccggta aagacgatga
    12001 taataccgcg ttcatcctgg gcctgggcca cacgagcatc tactttttct ttcactcgct
    12061 gctgacgctg ttggtagcgt tcatcactca ttgggaaatt cctggtttac ggcccggttg
    12121 ggcgtcaaag gtcatgccgg ttttacggcg gctgtcatcg cccatcagcc agaggtagag
    12181 cggcatgata tcagcgggtg ttttaagttt ctgtggatct tcggtcggga aggcgctggc
    12241 acgcattgcg gtgcgcgtac cgcctgggtt aatgcagttg acgcgcaggc gctgctgata
    12301 ttcatcggcc agtacctgca tcatcccttc ggtggcaaat ttcgacgctg catatgcgcc
    12361 ccagttggct cgtccctgac gtccaacgct tgatgaagta aagaccagtg aaccggcgtc
    12421 cgatttgagt aataaaggaa gcagtgcctg ggtgagcata aaggtggcat taacgttgac
    12481 ctgtatgacg tcctgccaga cctgtggatt ttgttcgctc attggacaaa catcgccgag
    12541 caatccggca ttatgcaaaa caccatccag acgcggataa ttaacggcaa tgcgctgtgc
    12601 cagttgttgg caatcttcgg acctgcaggt cagcaaatcg agaataaacc actgtggctg
    12661 acgcccagtt tcttcgttta tgtggctggc tacctgacgt aatttttctt cattacggcc
    12721 caacagaatc actgtcgcac catagcgtgc atacgtcatc gcggcttcac gaccaatacc
    12781 atcactggct cccgtcacca ggataatgcg atcattgagt aaatcttgtt ttggctggta
    12841 atgcatggct actcctcaac gacgttgtct gtcgcgctgc cagtgtactt tatgactttg
    12901 gggctttatg cctgaaacag aagggcattt caatcagcct ggggcgataa caccgcaaaa
    12961 tggatacttt gtcatacttt cgctgcaata acatctctgc gagacggctt aacatgcctg
    13021 ttgtaaactg tgagccaaag cgttgtttaa ccaaggtggg gactcgtgga attgttgtct
    13081 gaatatggtt tgtttttggc gaaaatcgtt accgttgtgc tagcgattgc ggcgattgcc
    13141 gccattattg tcaatgttgc tcaacgtaat aaacgccagc gtggcgagtt acgggtcaac
    13201 aatctcagcg aacagtataa ggagatgaaa gaagaactgg ccgcggcgct gatggactca
    13261 catcagcaaa aacagtggca caaagcgcag aagaaaaagc ataagcaaga agcgaaagca
    13321 gcaaaagcga aagccaaact gggagaggtg gcaactgaca gtaaaccccg cgtctgggtg
    13381 ctggatttta aaggcagcat ggacgcccat gaagtgaact cgctacgtga agagataacg
    13441 gctgtactcg cagcattcaa accgcaggat caggttgtac tacgtctgga aagccctggt
    13501 ggcatggtgc atggttacgg gttggcggct tcgcagctgc agcgtctgcg tgataaaaac
    13561 attcctttaa ctgttacggt agacaaagtc gctgccagcg gcggttacat gatggcctgt
    13621 gtggcggaca aaattgtttc cgcaccgttt gctattgtgg gttccattgg ggtggtggcg
    13681 caaatgccca actttaaccg cttcctgaaa agcaaagata ttgatatcga acttcacacc
    13741 gccgggcagt ataagcgtac gctgaccttg ctgggtgaaa ataccgaaga agggcgggag
    13801 aaattccgcg aagagctgaa cgaaacgcat cagttattta aagattttgt gaagcgtatg
    13861 cgtccgtctc tggatattga acaggtggca acgggtgaac actggtacgg acaacaggcg
    13921 gtagagaaag gcctggttga tgaaatcaac accagtgatg aagttattct tagcctgatg
    13981 gaaggccgtg aagtggtcaa tgtacgctat atgcagcgta aacgactcat tgaccgactc
    14041 accggcagcg cggcagagag cgccgatcga ttgttgttac gctggtggca gcgggggcaa
    14101 aagccattga tgtaaaagac aaacgcgagg gtaagacctc gcgttttgct ttaatcaacc
    14161 aggtgatatt tttctgaaag cacatgggcc aggtgtttga acatattaaa caccgcggtg
    14221 cttttggctg ttggcaatcc ttgttcatct aaaaagtagt cgccggtaaa taccagcacg
    14281 ccattacgct gcgtgacgcc ggtggcttca atccctgcga gcgtatcctc atgctcacga
    14341 atgattttgt tggcctcttt caacagcgtc tcgcggtcga tgggttgtgt ctctttgttc
    14401 attttttact ccttaaacaa ggacattagt ctacgccagg catggcttgc agacaaatat
    14461 accacgctgg tggcaagagc gccttactgg caactttgga ttttgcatgc taataaagtt
    14521 gcgtatcgga ttttatcagg tacagtgtga cgctttcgtc aatctggcaa tagatttgct
    14581 tgacattcga ccaaaattcc gtcgtgctat agcgcctgta ggccaagacc tgttaactca
    14641 gtcacctgaa ttttcgtgaa cagagtcacg acaaggggtt gatatccgca gagagcgagt
    14701 ccatatcggt aactcgttgc cagtggaagg tttatcaacg tgcgacgcat tcctggaaga
    14761 atcaaattag gtaaggtgaa tatgggtaaa gctcttgtca tcgttgagtc cccggcaaaa
    14821 gccaaaacga tcaacaagta tctgggtagt gactacgtgg tgaaatccag cgtcggtcac
    14881 atccgcgatt tgccgaccag tggctcagct gccaaaaaga gtgccgactc tacctccacc
    14941 aagacggcta aaaagcctaa aaaggatgaa cgtggcgctc tcgtcaaccg tatgggggtt
    15001 gacccgtggc acaattggga ggcgcactat gaagtgttgc ctggtaaaga gaaggtcgtc
    15061 tctgaactga aacaactggc tgaaaaagcc gaccacatct atctcgcaac cgaccttgac
    15121 cgcgaagggg aagccattgc atggcacctg cgggaagtga ttgggggtga tgatgcgcgc
    15181 tatagccgag tggtgtttaa cgaaattact aaaaacgcga tccgccaggc atttaacaaa
    15241 ccgggtgagc tgaatattga tcgtgttaat gcccagcagg cgcgtcgctt tatggaccgc
    15301 gtggtggggt atatggtttc gccgctgcta tggaaaaaga tcgctcgtgg tctgtctgcc
    15361 ggtcgtgtgc agtcggtggc agtccgcctg gtggtcgagc gtgagcgtga aattaaagcg
    15421 ttcgtgccgg aagagttctg ggaagtcgat gccagcacga ccacgccatc tggtgaagcg
    15481 ttggcgttgc aggtgactca tcagaacgac aaaccgttcc gtccggtcaa caaagaacaa
    15541 actcaggctg cggtaagtct gctggaaaaa gcgcgctaca gcgtgctgga acgtgaagac
    15601 aaaccgacaa ccagtaaacc tggcgctcct tttattacct ctacgctgca acaagctgcc
    15661 agcacccgtc ttgggtttgg cgtgaaaaaa accatgatga tggcgcagcg tttgtatgaa
    15721 gcaggctata tcacttacat gcgtaccgac tccactaacc tgagtcagga cgcggtaaat
    15781 atggttcgcg gttatatcag cgataatttt ggtaagaaat atctgccgga aagtccgaat
    15841 cagtacgcca gcaaagaaaa ctcacaggaa gcgcacgaag cgattcgtcc ttctgacgtc
    15901 aatgtgatgg cggaatcgct gaaggatatg gaagcagatg cgcagaaact gtaccagtta
    15961 atctggcgtc agttcgttgc ctgccagatg accccagcga aatatgactc cacgacgctg
    16021 accgttggtg cgggcgattt ccgcctgaaa gcacgcggtc gtattttgcg ttttgatggc
    16081 tggacaaaag tgatgcctgc gttgcgtaaa ggcgatgaag atcgcatctt accagcagtt
    16141 aataaaggcg atgctctgac gctcgttgaa ctgacaccag cccagcactt taccaagccg
    16201 ccagcccgtt tcagtgaagc atcgctggtt aaagaactgg aaaaacgtgg tatcggtcgt
    16261 ccgtctacct atgcgtcgat catttcgacc attcaggatc gtggctatgt gcgagtagaa
    16321 aatcgtcgtt tctatgcgga aaaaatgggc gaaatcgtca ccgatcgcct ggaagagaat
    16381 ttccgcgagt taatgaacta cgacttcacc gcgcagatgg aaaacagcct tgaccaggtg
    16441 gcaaatcacg aagcagagtg gaaagctgta ctggatcact tcttctcgga tttcacccag
    16501 cagttagata aagctgaaaa agatccggaa gagggtggta tgcgcccgaa ccagatggtt
    16561 ctgaccagca ttgactgccc gacttgtggt cgcaaaatgg ggattcgcac agcgagcacc
    16621 ggggtattcc ttggctgttc tggctatgcg ctgccgccga aagagcgttg caaaaccacc
    16681 attaacctgg tgccggaaaa cgaagtgctg aacgtgctgg aaggcgaaga cgctgaaacc
    16741 aacgcgctgc gcgcaaaacg tcgttgcccg aaatgcggca cggcgatgga cagctatctc
    16801 atcgatccga aacgtaagtt gcatgtctgt ggtaataacc caacctgcga cggttacgag
    16861 atcgaagagg gcgaattccg cattaaaggt tatgacggcc cgatcgttga gtgtgaaaaa
    16921 tgtggctctg aaatgcacct gaaaatgggg cgtttcggta aatacatggc ctgcaccaac
    16981 gaagagtgta aaaacacacg taagatttta cgtaacggcg aagtggcacc accgaaagaa
    17041 gatccggtgc cattacctga gctgccgtgc gaaaaatcag atgcttattt cgtgctgcgt
    17101 gacggtgctg ccggtgtgtt cctggctgcc aacactttcc cgaaatcgcg tgaaacgcgt
    17161 gcgccactgg tggaagagct ttatcgcttc cgcgaccgtc tgccggaaaa actgcgttat
    17221 ctggccgatg cgccacagca ggatccggaa ggtaataaga ccatggttcg ctttagccgt
    17281 aaaaccaaac agcaatatgt ctcttcggaa aaagacggaa aggcgactgg ctggtcagca
    17341 ttttatgttg atggcaaatg ggttgaagga aaaaaataac ctttaattct gtcaggttgt
    17401 tataaacaaa gggtcgcgaa agcggccctt ttttattgca tattattt
//
LOCUS       DAPHBJ010000091        17441 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.092, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000091 DAPHBJ010000000
VERSION     DAPHBJ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.092"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(194..1786)
                     /gene="lsrK"
                     /locus_tag="RHZ76_002649"
     CDS             complement(194..1786)
                     /gene="lsrK"
                     /locus_tag="RHZ76_002649"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HDV2813265.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRLAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVLVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPAPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1865..2818)
                     /gene="lsrR"
                     /locus_tag="RHZ76_002650"
     CDS             complement(1865..2818)
                     /gene="lsrR"
                     /locus_tag="RHZ76_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HDV2813266.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3067..4602
                     /gene="lsrA"
                     /locus_tag="RHZ76_002651"
     CDS             3067..4602
                     /gene="lsrA"
                     /locus_tag="RHZ76_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HDV2813267.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTMEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPVVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGNEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4596..5624
                     /gene="lsrC"
                     /locus_tag="RHZ76_002652"
     CDS             4596..5624
                     /gene="lsrC"
                     /locus_tag="RHZ76_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HDV2813268.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVTTLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLMAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5624..6616
                     /gene="lsrD"
                     /locus_tag="RHZ76_002653"
     CDS             5624..6616
                     /gene="lsrD"
                     /locus_tag="RHZ76_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HDV2813269.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6628..7581
                     /gene="lsrB"
                     /locus_tag="RHZ76_002654"
     CDS             6628..7581
                     /gene="lsrB"
                     /locus_tag="RHZ76_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HDV2813270.1"
                     /translation="MTLHRFKKIALLSVLGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDVLCPAL
                     KRDMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKVKVAFFYS
                     SPTVTDQNQWVKEVKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKKGSMKTGDKLDIQGV
                     GQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKENIGKYDF"
     gene            7608..8483
                     /gene="lsrF"
                     /locus_tag="RHZ76_002655"
     CDS             7608..8483
                     /gene="lsrF"
                     /locus_tag="RHZ76_002655"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HDV2813271.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGNTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8507..8797
                     /gene="lsrG"
                     /locus_tag="RHZ76_002656"
     CDS             8507..8797
                     /gene="lsrG"
                     /locus_tag="RHZ76_002656"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HDV2813272.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8853..9611
                     /gene="tam"
                     /locus_tag="RHZ76_002657"
     CDS             8853..9611
                     /gene="tam"
                     /locus_tag="RHZ76_002657"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HDV2813273.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLQQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRME"
     gene            complement(9615..10529)
                     /locus_tag="RHZ76_002658"
     CDS             complement(9615..10529)
                     /locus_tag="RHZ76_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HDV2813274.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10736..12187)
                     /gene="uxaB"
                     /locus_tag="RHZ76_002659"
     CDS             complement(10736..12187)
                     /gene="uxaB"
                     /locus_tag="RHZ76_002659"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDV2813275.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12414..13400)
                     /locus_tag="RHZ76_002660"
                     /pseudo
     CDS             complement(12414..13400)
                     /locus_tag="RHZ76_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            complement(13473..13832)
                     /locus_tag="RHZ76_002661"
     CDS             complement(13473..13832)
                     /locus_tag="RHZ76_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HDV2813276.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAAALPVNDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(13832..14758)
                     /gene="glsB"
                     /locus_tag="RHZ76_002662"
     CDS             complement(13832..14758)
                     /gene="glsB"
                     /locus_tag="RHZ76_002662"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HDV2813277.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(14822..16210)
                     /gene="sad"
                     /locus_tag="RHZ76_002663"
     CDS             complement(14822..16210)
                     /gene="sad"
                     /locus_tag="RHZ76_002663"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HDV2813278.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARHMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16311..17192
                     /locus_tag="RHZ76_002664"
     CDS             16311..17192
                     /locus_tag="RHZ76_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2813279.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLDGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGYAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVIAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLEVPDSARQGYQ"
     gene            17270..>17441
                     /gene="yneK"
                     /locus_tag="RHZ76_002665"
     CDS             17270..>17441
                     /gene="yneK"
                     /locus_tag="RHZ76_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HDV2813280.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATK"
BASE COUNT         4319 a         4471 c         4373 g         4278 t
ORIGIN      
        1 ttttcgttat aaaataaggg ttatgcttaa tgctttaaga aaaatagcaa tttccccttg
       61 aatatattac tggtgatgga aggatgaatc tggaagctgg catttaacaa gccagttcta
      121 aaagaaggga aataagaata actatactca aaaactaaca gccacggtca tcatgatgtg
      181 gctgtcaatg aaactataac ccaggcgctt tccataacga cgtcgtcagt ccatgatcaa
      241 ccagccccag ctgatcctga taaactgcct gccacttatc gcgtgaatcc tgataaagtt
      301 catgcttttc cggggctggt gtgtgcgtcc gttcccagcg aaccagccgt tctccggttt
      361 ctgccattga tgaaaaaatt ccggcaccga cgccagctgc aatggcacat cctaatgcag
      421 tggcttcttt gaccaccgga atattgacgg gtaatcccga gacatcagcg agaatttgac
      481 tccataattt cccttttgaa cctccgcctg caaagactaa cgatgaagga tgaatattcg
      541 aaaaatcagc aatttgctgc aagttacacg ctgaaacaat cgccgcattt tcttccagcg
      601 cacggaacaa tgtcgctttg ttacatttat ccgggtcaat ggacaaatta ataaaggaag
      661 gcgcagcgtg ataccaggtt ttaaagcgca ttctgtcgga gaagatcggc attacgcccc
      721 acgacccagg cggcacccga ctggccatct cttccagcag cgtataggtg tcgatgccta
      781 aacgttcggc aatcaatttt tcttcggcac agaaagcatc gcggaaccag cgcatggtga
      841 gtccggtaaa aaagcttata gattcagctt gtaccatgcc aggaattaca tggggattaa
      901 cgagcacgtt catttctggg tctgtcaccg gcgcggctaa gtttacaact tgctgccaga
      961 atgtgccgcc aagaaccgcg gtttgtgcca gacgcacaac gcctaacccc aggcaaccaa
     1021 gctgcacgtc gcctcctcca acgaccacag gagtgcccgc cttcagaccg cagagttccg
     1081 ccgcttgtga acttaccacg cccagcaatg tgccggtttc tttgacagga gaaagaatat
     1141 cggcacgcag gccagccata tccagcaatg caggtttcca gtcacgggtg gttagatcaa
     1201 gaagtcccgt ggtgccagcg ttagagggat ccaccgcaag ttcgccgctg agcatatagg
     1261 ccagccagtc gctgatcatg gtgatggttg atgcctgacg gtaaatatcg gaacgatggt
     1321 gcgccagcca aagtaatctg gggatggcac ttaaagccag tgtttgtccg gtcgcgcgat
     1381 aaacttcgtt ttcaaaggta ttgttgtgca gttctttaag ttcgctaact tcgcgcgccg
     1441 ctctggcatc cacattggcg caggcccaga tcggtgttcc ttcattgtta tataaaacaa
     1501 tgccttcacg catcgaacat gccgaaacgg cagcgatata ctccggggct atgccggcgt
     1561 tgtgcaacgc ctggcgcata cactcacacg ccagttgcca gtttttgttg agatcaaatt
     1621 ccatagaacc aggaacgtcc ggtactgcca gatgccgcca ctccgcctgt cccactgcta
     1681 tttgattgcc ttccaggtcg aatatcacag cccgaatact tccggtgcct gcatccagcg
     1741 ccatcaggta gtactttgat tctgaagggg taaagagtcg agccattaga ttatcctcgg
     1801 cttatgcgtt ctgtctgttc ctctatacgt tctccatcat tcccggtaat aaggtcatgc
     1861 aaatttaact acgtaaaatc gccgctgctg tgtcctgatc ggtaaccagt gcgttgatat
     1921 aaccgccttt cattgcagcg gcaattgctt cggctttatt ttctccccct gccacgccaa
     1981 cccggacggg tatggtcttc agcgcgctta aaggtaagcc aatcagttcg ttatgtattt
     2041 tgatatccgt gacaacgtca ccttttgcat caaaaaagta gcctaaaatg tcgccaaccg
     2101 ccccttttcg gccaatcatt aactgttcgc cctggctgat ataaccggag cgaatgatcg
     2161 tcgcgtcgtc ttgttgactc acggccccaa tgccgacaat cgccacatcc gctgcttgcg
     2221 cggctagcag aacatctttg acgcaatttt cattttttag cgtacgggca atgtcagcgg
     2281 aggatgcccg caacggagcc ggaataatat tcacactgca cgccgcgtta agctgcccga
     2341 tacccgtcat ataagaaccg acgccaccgg agagcgtgac caggcgaatt tgctgtgacg
     2401 aaataaaacc acttaagcgt tgcagcgtat tcatggttgc ctcgccaaaa ccaatcgcca
     2461 gcatctgttg tggttgaagt aaactcatca acatatgcgc cgcgcctatc cctagtcgcc
     2521 cgccgacatc agcatccgca agcccaggga tcacccggac atgttgcagc gaaaactgac
     2581 gacgtaattg agtttcatat tccagacagc cttcaaagcg agaattaatc tgtacgcgaa
     2641 taatgccgga ctgatgccct ttctccagca atcgcgatac tttcaaacgt gtcaggccga
     2701 gacgatcgct gatctcactc tgggtcagcc cgtcgtggta gtaaaaccac gcgatccgcg
     2761 cgacctgttc ttcttcacac attccctgtt ctgaaattac cgaatcgttg attgtcataa
     2821 ttcattcttc actttgaaca tatttaaatc tttaatgcaa ttgttcagtt cttgcgcatt
     2881 tatatctgtg atggcaacca cagcttgact ctacgagtat gaacaaacgc aaccgtgaaa
     2941 atcaaaatag cataaattgt gatctattcg tcggaaatat gtgcaatgtc cacctaaggt
     3001 tatgaacaaa ttaaaagcag aaatacattt gttcaaaact cacctgcaaa actgaacggg
     3061 ggaaatatgc aaacgagtga tacccgcgcg ttaccgctac tttgcgcccg ctcggtttat
     3121 aaacagtatt caggggtcaa tgtcctgaaa ggcatcgatt ttacgttgca tcagggggag
     3181 gtccacgccc tgctcggcgg caatggtgcc ggtaaatcga cgttaatgaa gattattgcc
     3241 ggtattaccc ctgctgatag cggtacgatg gagattgggg gcaacaacta cgccagatta
     3301 acgccagttc atgctcatca gctgggtatt tatctcgttc cccaggaacc gctgcttttc
     3361 ccaagcctgt cgataaaaga aaacatcctg tttgggctgg caaaaaaaca gctctccatg
     3421 cagaaaatga agaacttgct ggcggcgctg ggctgccagt ttgatctgca tagtctggca
     3481 ggatcgctgg atgtcgccga tcgccaaatg gtggaaatcc tccgcgggct gatgcgcgac
     3541 tcgcggattc tgatcctcga tgaacctacc gcctcgctta cccctgcgga aaccgaacgc
     3601 ttgtttactc gcttgcaaga gctgcttgct actggcgtgg gtattgtttt tatctcgcat
     3661 aagctgccgg aaattcgcca gattgccgat cgaattagcg tgatgcgcga cggaaccatc
     3721 gccttaagcg gcaaaaccag cgaactgtct accgacgaca ttattcaggc catcacgcca
     3781 gtggtacggg aaaaatcgct ctctgccagc caaaaattat ggctggagtt acctggtaac
     3841 cgcccacaac atgccgccgg aacgccggtg ctgacactgg aaaatctgac tggcgaaggc
     3901 ttcaggaatg tcagcctgac gctcaatgcc ggagaaattc tgggcctggc tgggctggtg
     3961 ggagccggac gcacagaact ggccgagacg ctctatggtc tgcgtacttt gcgtggcgga
     4021 cgcattatgc tgaatggtaa cgagatcaat aaattatcca ctggagaacg tttactgcgc
     4081 ggtctggttt atctgccgga agatcgccag tcatccggac tgaatctcga tgcttcgctg
     4141 gcctggaatg tctgcgccct tactcataac cttcgtggat tctgggcgaa aaccgcgaaa
     4201 gataatgcca ccctggaacg atatcgtcgg gcgctgaata ttaaatttaa ccaaccggaa
     4261 caagctgcac ggacattatc cggtggcaac cagcaaaaaa tcctcattgc caaatgcctg
     4321 gaagcctcgc cgcaagtatt gattgtcgat gagccgacgc gcggcgtgga tgtctcggcg
     4381 cgtaatgata tctaccaact gttgcgcagc atcgccgcac agaatgtggc tgtgctgctt
     4441 atctcctccg acctggaaga gatcgaactg atggcagatc gcgtgtatgt gatgcatcag
     4501 ggcgaaattg cccactctgc actgaccggg cgcgatatta atgtcgagac tattatgcgc
     4561 gttgccttcg gcgatagtca gcgtcaggag gcgtcatgct gaagtttatt cagaacaacc
     4621 gtgaaatcac ggcactgctg gcggtggtgc tgctgtttgt attacccggt tttctcgacc
     4681 gccagtattt aagtgtgcaa acgctgacca tggtttatag cagcgcgcaa atcctgatcc
     4741 tgctggcaat gggcgcgacg ctggtaatgc ttacgcgcaa tattgatgtt tcagtgggtt
     4801 cgattaccgg aatgtgcgcg gtgctgttgg ggatgttact gaacgcagga tattcactac
     4861 ctgttgcctg tgtcacgact ttactgcttg gtttgctcgc gggatttttc aacggtgtcc
     4921 tggtcgcgtg gctaaagatt cctgccattg ttgccaccct tggcacgtta gggttgtaca
     4981 gaggcatcat gttgctgtgg actggcggta aatggattga agggttaccc gccgaactga
     5041 aacagctctc cgcaccgctg ctgtttggcg tttccgcgat tggttggttg acgataattc
     5101 tggtggcatt tatggcctgg ctgatggcga agacggcgtt tggacgcagt ttttatgcca
     5161 cgggcgataa tttacagggc gctcgtcaac tgggcgttcg tacggaagcc attcgcattg
     5221 tggcattttc gttgaacggc tgcatggcgg cactggcggg aattgtgttt gcttcgcaga
     5281 ttggttttat ccccaaccag accggtaccg ggctggagat gaaagcaatt gcagcctgcg
     5341 tgctgggcgg cattagtttg ctcggcggtt ccggtgcgat catcggtgcg gtactcggcg
     5401 catggttcct gacgcagatc gatagcgtac tggtgctgtt gcgcattccg gcatggtgga
     5461 acgattttat cgcgggcctc gtgctgctgg cggtgctggt gtttgatgga cgcctgcgtt
     5521 gtgcgctgga acgtaatcta cggcggcaaa aatatgcccg ctttatgacg ccaccgccat
     5581 ccgttaaacc cgcttcgtca ggtaaaaaac gggaggccgc ataatgcgta ttcgctacgg
     5641 ttgggaactg gctcttgccg cactgctcgt tattgagatt gtcgcatttg gtgcaattaa
     5701 cccgcggatg ttagatctca atatgttgct gttcagcacc agtgacttta tctgcattgg
     5761 cattgtcgcc ctaccgctga cgatggtgat tgtcagtggc gggatcgata tttcgtttgg
     5821 ttcgaccatc ggcctctgcg ccattgcatt gggcgtactg tttcaaagtg gtgtgccgat
     5881 gccgctggcg atactcctga ccttactgct cggcgcattg tgcgggctga tcaacgccgg
     5941 attaattatc tataccaaag ttaacccgct ggtgattacg cttggcacgc tgtatctgtt
     6001 tgccggaagc gctctgctgc tttccggtat ggccggagcg acggggtacg aaggtattgg
     6061 tggattcccg atggcgttta cagatttcgc taacctggat gtgctgggac tccccgttcc
     6121 gctgattatc ttcctgatat gtctcctcgt tttctggctc tggctgcata aaacccatgc
     6181 cggacgtaat gtgtttttga ttgggcaaag cccgcgcgtg gcgctttata gcgcgattcc
     6241 agttaaccgc accttatgtg cgctctatgc catgacgggg ctggcgtctg cggtcgccgc
     6301 tgtgctgctg gtatcgtatt ttggttcagc acgttccgat ctcggtgcgt cgtttctgat
     6361 gcccgccatc accgccgtgg tgcttggcgg tgccaatatt tatggtggtt ccggttccat
     6421 tatcggcacc gccattgcgg ttttattagt gggatatttg caacaaggtt tgcaaatggc
     6481 aggagtgcca aatcaggtgt ccagcgccct ttccggtgcg ctacttatcg tcgttgtcgt
     6541 aggtcgttcc gttagcctgc atcgccagca aattaaagag tggctggcgc gtcgggccaa
     6601 taacccattg ccataaagga tatcttcatg acacttcatc gctttaagaa aatcgcctta
     6661 cttagcgttc ttggcattgc cgcaatctct atgaatgtgc aggccgcaga gcgtattgca
     6721 tttattccca aactggttgg cgtgggattt tttaccagcg gtggcaacgg cgcacaacaa
     6781 gcgggtaaag agctgggcgt tgatgtgacc tacgacgggc cgacagaacc cagtgtttct
     6841 ggtcaggtac agttgattaa taacttcgtc aatcaaggtt ataacgccat tatcgtttct
     6901 gcggtttcgc ctgatgtctt gtgtccggca ctgaaacgcg acatgcaacg tggtgtgaga
     6961 gtgctgacct gggactctga tactaaaccg gagtgccgct cttactacat taatcaggga
     7021 acgcccgccc agttgggagg tatgttggtg gatatggcgg cgcgtcaggt gaataaagac
     7081 aaagtcaaag tcgcgttttt ctactcaagc cccaccgtta cggaccaaaa ccagtgggtg
     7141 aaagaagtga aagcgaaaat cgccaaagag catcctggct gggaaattgt cactacgcag
     7201 tttggctata acgatgccac taaatcatta caaaccgcag aaggaatatt aaaagcgtat
     7261 agcgatctcg acgccattat cgcccccgat gccaacgccc tgcccgctgc cgcacaagcc
     7321 gcagaaaact tgaaaaatga caaagtagcg attgtcggat tcagtacgcc aaacgtgatg
     7381 cgtccatatg tggaacgcgg cacggtgaaa aaaggatcaa tgaaaacggg cgacaagctg
     7441 gatatccagg gcgtaggtca ggttgaagtc tcgccaaata gcgttcaggg ctatgactac
     7501 gaagcggatg gtaatggcat cgtactgtta ccggagcgcg tgatattcaa caaagagaat
     7561 atcggcaaat acgatttctg atgtgcatta cttaaccgga gtaagttatg gcagatttag
     7621 acgatattaa agatggtaaa gattttcgta ccgatcaacc gcaacaaaat atccctttta
     7681 ccctgaaagg ttgcggtgcg ctggattggg gaatgcagtc acgcttatcg cggatattta
     7741 atccgaaaac gggtaatacc gtgatgctgg cctttgatca tggttatttt caggggccga
     7801 ctaccggact tgaacgcatt gatataaata tcgccccgct gtttgaacat gccgatgtat
     7861 taatgtgtac gcgcggcatt ttgcgcagcg tagttccccc agcgaccaat aagccggtgg
     7921 tactgagggc gtcaggtgcg aactctattc tggcggaatt aagtaatgaa gccgtggcgt
     7981 tatcgatgga tgatgccgtg cgcctgaaca gttgcgcggt ggcggcgcag gtttatatcg
     8041 gcagcgaata tgaacatcag tcgatcaaaa atattattca gctggttgat gccggaatga
     8101 aagtgggaat gccgaccatg gccgtgactg gcgtgggcaa agatatggtg cgcgatcagc
     8161 gttatttctc gctcgcgact cgaatcgccg ctgaaatggg ggcgcaaatt atcaaaacct
     8221 attatgtcga aaaaggtttt gaacggattg ttgccggatg tccggtaccc attgttattg
     8281 ctggcggtaa aaaattaccg gagcgcgagg cgctggaaat gtgctggcag gctatcgatc
     8341 agggcgcttc tggtgtggat atggggcgta atattttcca gtctgaccat ccggtggcga
     8401 tgatgaaagc cgtacaggcg gtggttcacc ataacgaaac ggctgatcga gcatatgaac
     8461 tctatctgag tgaaaaacag taactgcgga tctaaggaga agaattatgc acgtcacact
     8521 ggttgaaatt aacgttcatg aagacaaggt tgacgagttt atcgaagttt ttcgccagaa
     8581 ccacctgggc tctgtacagg aagaaggcaa tttgcgcttc gatgtcttac aggacccgga
     8641 agtgaattcg cgcttttata tctacgaagc ctataaagat gaagacgcag tggcgttcca
     8701 taaaaccacg ccccactaca aaacctgtgt cgcgaaactg gaatctttaa tgaccgggcc
     8761 gcgtaaaaaa cgtctgttca atggtttgat gccgtgaggc gaatttatca attttatcta
     8821 caattggggt aacgcgctga cgggagtaaa aaatgtctga ctggaacccc tctttatatt
     8881 tgcacttttc cgctgaacga tcgcgtccgg cggtggagct gcttgccaga gtgccgctgg
     8941 aaaatgtcga atatgttgcc gatcttggct gtggcccagg taacagcacc gcccttctac
     9001 aacaacgttg gcctgcggcc aggataacag gcatcgactc gtctccggcg atgattgctg
     9061 aagcgcgcag tgctttgcca gactgccagt tcgtggaagc agacatccgc aactggcaac
     9121 cggtacaggc actcgatctg atttttgcta atgcctcact gcaatggctg cccgaccact
     9181 acgaattgtt tcctcatctg gtttctttac ttaatccgca gggcgtgctg gcagtacaga
     9241 tgccagataa ctggctggag ccgacccatg tgctcatgcg cgaagttgcc tgggaacaaa
     9301 actacccgga tcgcgggcgt gagccgttgg ctggcgttca tgcttactac gatattttga
     9361 gcgaagccgg atgtgaggtc gatatctggc gaaccaccta ctatcaccag atgccgtcac
     9421 accaggcgat tatcgattgg gtgactgcca ctggattacg tccgtggtta caggatctga
     9481 ccgagagcga acagcagctt tttcttaagc gctaccatca gatgctggaa gagcagtatc
     9541 cactgcaaga gaacggacag atactgctgg catttccgcg tctgtttatt gttgcccggc
     9601 gtatggagta aatatcacgt cagctggtaa tgacgatcgg gaagaatttt tgctggaatt
     9661 tcggcttcat cgttcatctg taacaggtca atttcaatag cgttgcagat ggcatccagt
     9721 ggtaaatcat tgttttcagt accgaacgga tcttccagtt cttccgccag acaatccagc
     9781 gaaataaaag tgtaggaaat cagcacagag ataaaaggcg tcatgtaatg caggtccacg
     9841 accagcgcga acggcagcat gatacaaaac agataaacgg tacgatgcaa aatcagggtg
     9901 taggcaaagg gaattggcgt gtaggcaatg cgctcacagc ctgccagaac cgctgaaata
     9961 tcattaagcc gatcgttgag gctaataaac aggatatctg aaagctgccc attgcggcgc
    10021 tgaaccgcca accactcccc cattattaac aagatacggt tagctggaga gttcgaagcc
    10081 agtacaggct gaagatcttc agtcttgaga taatgagcca gcacttccgc ctgtggctgt
    10141 ttgcgtaatg tcatgcgtaa acagtgggcg aaggcgattt gcaaccgggc aaactccctt
    10201 acacttgccg aatccggcaa tgtcgttttt acctcgcgca gtaacgaccg tgaggcaatc
    10261 atcaactgcc cccaaagttt tcgcgcttca acgtaacggg cgtacccggc attattacga
    10321 aaaccaagaa aaatggcgat ggcgacaccg agaatgctga acggtgcgag ggtgaatttg
    10381 atgcccagat gcgtgtacca gggcagcatg aaaataacag cgatagaaaa gagaaaattg
    10441 agtagtaagc gcgatgatat cttggataat actgagccgt gccagacaaa aatacggcgc
    10501 agccagtgtt gttgtggacg aacaatcatg gttatcttca ggcgtgaaaa agtcgcccta
    10561 ttaaacgtga ttacgatcac attctcaaga cgctacttac aaattaccta caagcttaca
    10621 acattaacag gcgctgcatg tggcatctgt taatgataaa ggcacatagg atgttgtaac
    10681 taatatagtg aaataagaaa cccggtcgaa accgggttca gaagtaacgg tgttattagc
    10741 acaacggacg tacagcttcg cgcatccctt tttcaagaat cgcatccagg tcattagcaa
    10801 cctgctcgac cagaccaggc acttgcgtca ggtcctgctc ccagtggtct ttttctgcca
    10861 aaacaatcgc taccagttcc tgggtgccga taacacggtc acgatgctgg ctccagagtt
    10921 gctgataacg ttccagccag tgtgcatcgt cctgtaccgg atatgtttct ccgttacgct
    10981 caccgcgata gaaggcaatc aatgccgcta atgcgaaagt aaggcgcgcc ggaagtgtgc
    11041 cttttgcctt ctgccctgcc agcagctgcg gcaggatgcg agtgcggaac ttggtcatac
    11101 cgttgagcgc gatagacagc agctgatgct taatgtacgg gttacggaaa cgcccggtga
    11161 ctgcactggc gaaagattcc agttcatcac gaggcaaatc cagtaccgga ataatttctt
    11221 cgtagatagc tttttcaacg aatgcgcaaa tttcagcatc gttcatcgct tcacctacgg
    11281 tatccagccc tgcctggaac gccaccggca ccagcgcggt gtgcgcaccg ttgaggattg
    11341 ccactttgcg ttctttgtac ggtttaatat cgtcaacaat cagcacgttg agcggatatt
    11401 tgtccagacg cagttcagtc gctaaggatt tcggtccctg aatcacaaac aggtaaaagt
    11461 gttcagcggt gtcgagaaaa ccatcgtgat aacccagttc ttcttccagt ttagccactt
    11521 catcgcgcgg ataaccggta acgatacggt ccaccagcgt agaacagaag ctgttagcct
    11581 gatccagcca ttgaataaat gcttctggca gtgcccactc ttgcgcatag cgcagcacca
    11641 gttcacgcaa cgcgtcgcca ttgtagtcaa tcaactcaca cggaatgatg atccaacctt
    11701 tatccagcgc accgttgaaa tggctaaagc gttcgaacag cagacgggtc agttttgccg
    11761 gatagcttac cgctggcgcg tcatcgaatt tatcgcccgc atggtagctg atacccgctt
    11821 cggtggtgtt agagaaaaca aagcgcattt ccgggttgtg cgccagtttc aggaattcat
    11881 cgtattcact gtagacgctg atttcacgat taaccgagcg aatcagacgc gcgtcgctga
    11941 ccgcttcccc cttctcattc aggccacgga taatggtggt gtacagacca tcctgcgtgc
    12001 tcagtgacgg cgggaatgaa gtttcaatcg gacgaacaac gaccacgcca gaattcagat
    12061 cggtgtgctc attcaggaga tcgatttgcc agtcaacaaa ggcgcgcagg aagttacctt
    12121 caccaaactg aatgatacgt tctggatact gtgcaccggg aaaatcgcga cgatttagtg
    12181 ttttcacaat gggttccctt ctgattagtc atacaacctg tttgaattgg tacgacaggt
    12241 tagcaaactt taatacgccg aacccctgtt ttgatcaact cctgatgatt aatgagcagt
    12301 tttatgagaa aagtgtggcg cggatcatgg tttaatcgag gaaaaacgcc ttttcctgga
    12361 tcataaagtg gtagaacaca ttgcattcaa atcgcgcgta atgaataaag atgtcagaca
    12421 acttcctcac cgtaacgcat agtgctggta cggttgcgcc catctttctt cgagcgatac
    12481 agacacgtat cggcttcgac catcaattta ttaaagtcat cggtcagcgt tcggtacgat
    12541 gcgcgaccac taccgacgcc aatacttacc gtgagataaa gcgttttttg ttgccaggtg
    12601 aatggttgca gttcaacgcc tttacggatt ttttccgcca ttagcagacc atcgacagga
    12661 ttcaccgacg gcactgcaac agcaaattct tcgccgccca ttcgcgccac cagcccctta
    12721 tcaccgacaa tcttctgaat atgccgggca aacacgctta acactttatc gccacattca
    12781 tgtccatagt tatcgttaat gcttttgaaa tagtcgatat caagcagcat gacagtcaga
    12841 tgttgtgtct gtttcagcga tggacttttc agcgcctcat aaagaccgga ccgtgagtaa
    12901 acctgagtca gaaaatcaaa gtcggctcgc agcgcaactt gcttcattag cgaattgatc
    12961 gctgccacgc taaaagaaac cataattggg catatcgcca tcgtggcaat accgagacgt
    13021 gcggagaaca tttgcggaat ggagaacggc gaaccgaccg agatatcaat caccgaattt
    13081 gccaccagta cgatttccac cgcaccggtg acaaaggtca acagacatgt tacctgcggc
    13141 gtatagcgca ctgcacacca gattaatgca ggcagcggga acgccagact ccccgctccg
    13201 ccaatgacta ccgaggcaat aaccgacaca ataagcgcga tggcgggcat catctgttct
    13261 gctttaaagc gcggcagcac tccaggaata gccaacgtca gcatacaagg cacgatcaac
    13321 acgcccgttg agaattgctc actgaaccag tccgcaagca aaggccagaa atccagacta
    13381 tcaatactga ccgaaccaag atagcatgtt cgctgacagc ggcggcaaat aaaggtaccc
    13441 tgggaatgct attttgtcgt tttccgcaat tctcatggct gattcatctg tacaaccagc
    13501 cagtgataga ttaccgccac gatataacgt tgttgttctt cgcttaacga aacaccctgc
    13561 ggaatgttat gccacttagc cagacaaccg cgacagcagg tcgcggtggc gtgctgagcg
    13621 ataaacaccg gatgcccgcg catgggggtt tgcttaccgt cattaaccgg taatgccgca
    13681 gcaagacgtt tagcgacaaa atccgccgca tgttgttcga tgactggcgc gcctttctcc
    13741 agacaatact gacgctcttt catgcccaaa cgaaagcgag agcgaaattt tgaacgggat
    13801 aaacgcgcga agagaggatc gagagactgc attaataaac cgaacgccct aactgtttcg
    13861 tcaattgttc aaggacggca atgcccgcaa gcgagttacc tgcatcatcc agttccggac
    13921 tccagactgc gatggccatt tcatgcggaa caatcgccac aataccgccc ccaacgccag
    13981 atttcgccgg tagtcccacc cgccaggcaa actcccccgc gttctggtac ataccactgg
    14041 tcgccatcag cgcgttaatt tgccgcgcct gcattggcgt caccactggt tcatcaatat
    14101 gaatagcttt cccctgatta gccagaaaga caaacgtccg ggccagctct acacagctca
    14161 ttttcagagc gcagtaatga aagtagtttt gcagaacggt tgtcacgtca tgatggaaat
    14221 tgccaaacga cttcatcagc caggcgatag ccgcatttcg cgcggaatgt tcaaattcgg
    14281 aacgcgctac caccgtatcg taggaaatat cagacacacc gcttaagccg cgcacgactt
    14341 ccagcatacg ttgccgtggt gcgcttaatc gcccttgcaa catatcgcag accaccagcg
    14401 caccggcatt aatgaacgga ttacgcggta taccctgctc catttccagt tgcactaagg
    14461 aattgaacgg tgatccagac ggatctttgc cgacgcgttg ccagatttcc tcttcggagt
    14521 aatgacgcat ggcgacaacg agactcagca ctttggaaat agactgaatg gaaaaacgtt
    14581 cttgcgcgtc tccggcctga aaaagctgtc cgtcaacggt acagatagca atccccaatc
    14641 gggaaccgtc tactgtagcc agcgccggaa tataatccgc gactttaccc tgaccaatga
    14701 gcggccgcac ttgccgcaag atgttttcta aaattgcatt atccatggcg actgccactt
    14761 tctactcctg gaccgcaggt ctgaaaagac ctgcgagtat atcagagctg aatatgtcgc
    14821 gtcagatccg gtctttccac accgtctgga tattacagaa ttcgtgtaag ccgaaatggg
    14881 aaagctcacg accaaagcca ctctttttca cgccaccaaa ggccactcgc gcgtcgctgg
    14941 cacaataacc attgataaac accccaccgc attccagacg tgccgccata tgtctggcct
    15001 gtgtttcgtc agtggtaaaa atggtcgctg aaaggccgaa ctcactatca ttagccagtt
    15061 ccagtgcatg ttctgcatct ttcgcaatgg tgattgccgc aacggggcca aacatttctt
    15121 cccgaaacgc ggtcatttct ggggtaacat tcgccagaac cgttggcgga tagtagttac
    15181 ctgccccagc catcttttcc ccgcccagta acaaacgcgc accctgcgcc agggttttct
    15241 ccacctgatg atgcagctca tcacgtaaat caaaacgagc cattggtccg agagcgttct
    15301 cttcgtcacg gggatcgccc attttcaagg ctgccgcagc tgccacaaaa cgttcggtaa
    15361 atgccgaagc aattccctct tcgataataa agcgttttgc cgctgcacat acctgtccgg
    15421 tattctgata acgtccggct accgccgctt tcaccgccag ttccagatcg gcatcgttaa
    15481 gcacaataaa cggatccgaa ccgcccagtt ccagtacgca ttttttcagt gccgctccag
    15541 cctgtgcgcc aatagccgct cccgcacgaa cacttccggt caccgtgaca gcagcaatgc
    15601 gcgagtcttt aatcatctga ctgacaccgt cgttgtcggc attcagccag ccatatacgc
    15661 cttgtgggat acccgcatct ttaaacacct gggcaatgag ctgtgcacag cccatcacat
    15721 tcggcgcatg tttaagtaag tagccgttac ctgcaagaat gatgggaaca gcgccacgca
    15781 tcacctgcca taacggaaaa ttccacggca taatcgccag aatcgtcccc aacggtcgat
    15841 actcaataac cgcctgctga ttttccacca gcgtaggttc cgccttcagc attgccggac
    15901 catgttctgc ataccagtca cacaaattcg ccgatttcgc cacttcagcg cgcgcctggt
    15961 tgattggttt gcccatttcg cgggtgatca tttgcgccat ttcttcgcta cgagcgcgca
    16021 gagccttacc gatatcacgc agtttttcag cacgataatc tatatttgtc tcgcgccagt
    16081 cgcgaaagcc tgctgccgcc agctgaagtg cgttttcgat atcgtcagcg ccagcccacg
    16141 gcagcacaga aagttgttca cccgtggcag gatttatcga aattgcatga gttgccggag
    16201 taatggtcat cggggtatct cctttatgag tcatggtatg aagatacgca gatttactct
    16261 tgctttaaaa tgaataatat taagccactt attcacgaat cgagaatgct atggatctga
    16321 cccaactgga gatgttcaac gcggttgccg aagctggcag cataacccag gctgcagcaa
    16381 aagtgcatcg cgtgccgtcg aatctcacta cccgtctgcg ccagttggaa acagaactgg
    16441 gggttgatct gtttattcgc gagaatcagc gtttacgtct ctctcctgcc gggcataact
    16501 ttttacgcta tagccaacaa attctcgcgt tagtggatga agcgcggagc gttgtcgctg
    16561 gcgatgagcc gcaaggttta ttttctcttg gttcgctgga aagcaccgct gcagtgcgca
    16621 ttcctgccac gctggcggaa ttcaaccgtc gttatcccaa aattcagttt tcactttcca
    16681 ccggcccttc cggcactatg ctggatggtg tactggaggg aaaactgaat gcggcgttta
    16741 ttgatggacc cattaaccat actgccatcg acgggatacc ggtataccgc gaggaactga
    16801 tgatcgtcac gccacaagga tatgcgccag taacccgtgc cagtcaggtt aatggcagta
    16861 acatttatgc cttccgcgcc aattgttcgt atcgtcgcca cttcgagagc tggtttcatg
    16921 ctgacggtgc cgctccggga actatccatg agatggagtc ttatcacgga atgttggcct
    16981 gtgtgatcgc aggagcaggc attgcgctta ttccgcgctc tatgctggaa agtatgccgg
    17041 ggcatcacca ggttgaagcg tggccgttag ctgagcaatg gcgttggtta acaacctggc
    17101 tggtctggcg tcgtggtgcg aaaacgcgcc cgctcgaggc atttattcaa ctgctggagg
    17161 tgcctgactc ggcaagacag ggatatcaat gagctatttt tgatagttct tgcggctaat
    17221 atgctctata tagtgatgtt ccgatgactt atgactatat ggggcaaata tggttacgcc
    17281 agtaagcatc agcaattaca tatctcttcc tgatgatttt cccgtacgta atattgcacc
    17341 tcaggtaaaa gaagttttaa aggattttat tgatgcactc agtaccataa tatgtgatga
    17401 agagtggcgc acgtctttaa acatcaactc agccacaaaa a
//
LOCUS       DAPHBJ010000092        16637 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.093, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000092 DAPHBJ010000000
VERSION     DAPHBJ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.093"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            118..2163
                     /gene="paaZ"
                     /locus_tag="RHZ76_002666"
     CDS             118..2163
                     /gene="paaZ"
                     /locus_tag="RHZ76_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HDV2813281.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSATTGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNTLLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAIQGSPSMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLESLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            2411..4684
                     /gene="tynA"
                     /locus_tag="RHZ76_002667"
     CDS             2411..4684
                     /gene="tynA"
                     /locus_tag="RHZ76_002667"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HDV2813282.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQLFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDN"
     gene            complement(4743..6242)
                     /gene="feaB"
                     /locus_tag="RHZ76_002668"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(4743..6242)
                     /gene="feaB"
                     /locus_tag="RHZ76_002668"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HDV2813283.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADRLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCGKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            6478..7383
                     /gene="feaR"
                     /locus_tag="RHZ76_002669"
     CDS             6478..7383
                     /gene="feaR"
                     /locus_tag="RHZ76_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HDV2813284.1"
                     /translation="MNPAMDNEFQQWLSQINQVCGNFTGRLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRLEW
                     IAAETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(7555..7881)
                     /locus_tag="RHZ76_002670"
     CDS             complement(7555..7881)
                     /locus_tag="RHZ76_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HDV2813285.1"
                     /translation="MKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVTDINAERKASYQQLAKQNNVSADDIAKLAGQKLVARAKPGEYVQGING
                     KWMRKS"
     gene            complement(7889..8074)
                     /locus_tag="RHZ76_002671"
     CDS             complement(7889..8074)
                     /locus_tag="RHZ76_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HDV2813286.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(8071..10710)
                     /locus_tag="RHZ76_002672"
     CDS             complement(8071..10710)
                     /locus_tag="RHZ76_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HDV2813287.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            10918..11907
                     /gene="ldhA"
                     /locus_tag="RHZ76_002673"
     CDS             10918..11907
                     /gene="ldhA"
                     /locus_tag="RHZ76_002673"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDV2813288.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            12018..12440
                     /gene="hslJ"
                     /locus_tag="RHZ76_002674"
     CDS             12018..12440
                     /gene="hslJ"
                     /locus_tag="RHZ76_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HDV2813289.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(12437..12703)
                     /locus_tag="RHZ76_002675"
     CDS             complement(12437..12703)
                     /locus_tag="RHZ76_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HDV2813290.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            12977..16501
                     /gene="nifJ"
                     /locus_tag="RHZ76_002676"
     CDS             12977..16501
                     /gene="nifJ"
                     /locus_tag="RHZ76_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDV2813291.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVIDELLTRGENVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         4159 a         4300 c         4164 g         4014 t
ORIGIN      
        1 ttttccgcag ttgctataca gatcgcatag ttaacatttc gttaaaagat cccttgcttt
       61 ttatgattcg cgatttaact attaacaaca gaaatgtgaa acatctggag agtagcgatg
      121 cagcagttag ccagtttctt atccggtacc tggcagtctg gccggggccg tagccgtttg
      181 attcaccacg ccattagcgg cgaggcatta tgggaagtga ccagtgaagg tcttgatatg
      241 gcggctgccc gccagtttgc cattgaaaaa ggtgcccccg ccctccgcgc gatgaccttt
      301 atcgaacgtg cggcgatgct taaagcggtc gctaaacatc tgctgagtga aaaagagcgc
      361 ttctatgctc tttctgcgca aacaggcgca acgcgggcag acagttgggt tgatattgaa
      421 ggcggtattg ggacgttatt tacttacgcc agcctcggta gccgggagct gcctgacgat
      481 acgctgtggc cggaagatga attgatcccc ttatcgaaag aaggtggatt tgccgcgcgc
      541 catgtactga cctcaaagtc aggcgtggca gtgcatatta acgcctttaa cttcccctgc
      601 tggggaatgc tggaaaagct ggcaccaacg tggctgggcg gaatgccagc catcatcaaa
      661 ccagctaccg cgacggccca actgactcag gcgatggtga aatcaattgt cgatagtggt
      721 cttgttcccg aaggcgcaat tagtctgatc tgcggtagtg cgggcgacct tttggatcat
      781 ctggacagcc aggatgtggt gactttcacg gggtccgcta cgaccggaca gatgctgcga
      841 gttcagccaa atatcgttgc caaatctatc cccttcacga tggaagctga ttccctgaac
      901 tgctgcgtac tgggcgaaga tgtcaccccg gatcaaccgg agtttgcgct gtttattcgt
      961 gaagttgtgc gtgagatgac cacaaaagcc gggcaaaaat gtacggcaat ccggcggatt
     1021 attgtgccgc aggcattggt taatgctgtc agtgatgctc tggttgcgcg attacagaaa
     1081 gtcgtggtcg gtgatcctgc acaggaaggt gtgaaaatgg gcgcactggt aaatgctgaa
     1141 cagcgtgctg atgtgcagga aaaagtgaac acattgctgg ctgcaggatg cgagattcgc
     1201 ctcggtggtc aggcggattt atctgctgcg ggtgcattct tcccgccaac cttattgtac
     1261 tgtccgcagc cggatgaaac accggcggta catgcaacag aagcctttgg ccctgtcgca
     1321 acgctgatgc cagcacaaaa ccagcaacat gctctgcaac tggcttgtgc aggcggcggt
     1381 agccttgcgg gaacgctggt gacggctgat ccgcaaattg cgcgtcagtt tattgccgac
     1441 gcggcacgta cgcatgggcg aattcagatc ctcaatgaag agtcggcaaa agaatccacc
     1501 gggcatggct ccccactgcc acaactggta catggtgggc ctggtcgcgc aggaggcggt
     1561 gaagaattag gtggtttacg agcggtgaaa cattacatgc agcgaaccgc tatacagggt
     1621 agcccgtcga tgcttgccgc tatcagtaaa cagtgggtgc gtggtgcgaa agtcgaagaa
     1681 gatcgtattc atccgttccg caaatatttt gaggagctgc aaccaggcga cagcctgctg
     1741 actccccgcc gcacaatgac agaggccgat attgttaact ttgcttgcct cagcggcgat
     1801 catttctatg cacatatgga taagattgct gctgccgaat ctattttcgg tgagcgggtg
     1861 gtgcatgggt attttgtgct ttctgcggct gcgggtctgt ttgtcgatgc cggtgtcggt
     1921 ccggtcattg ctaactacgg gctggaaagc ttgcgtttta tcgaacccgt aaagccaggc
     1981 gataccatcc aggtgcgtct cacctgtaag cgcaagacgc tgaaaaaaca gcgtagcgca
     2041 gaagaaaaac caacaggtgt ggtggaatgg gctgtagagg tattcaatca gcatcaaacc
     2101 ccggtggcgc tgtattcaat tctgacactg gtggccaggc agcacggtga ttttgtcgat
     2161 taatcggtga atgaagggca acggcgaata gttgcccttt tatttcacta agttttgtga
     2221 cgttgtcaca ttatgcatga tgtgtacatc tattttcagg gcatccactg tatgaaaagc
     2281 tggcacacct gccaaacaac ctggcaggtg caggcaatcc cctttgcatc agtactgata
     2341 atgtgaacct gactaaaccg cccacagagc gcggttgcta acaagaacac aacatctgac
     2401 gaggttaata atgggaagcc cctctctgta ttctgcccgt aaaacaaccc tggcgttggc
     2461 agtcgcctta agtttcgcct ggcaagcgcc ggtatttgcc cacggtggtg aagcgcatat
     2521 ggtgccaatg gataaaacgc ttaaagaatt tggtgccgat gtgcagtggg acgactacgc
     2581 ccagctcttt accctgatta aagatggcgc gtacgtgaaa gtgaagcctg gtgcgcaaac
     2641 agcaattgtt aatggtcagc ctctggcact gcaagtaccg gtagtgatga aagacaataa
     2701 agcctgggtt tctgacacct ttattaacga tgttttccag tccgggctgg atcaaacctt
     2761 tcaggtagaa aagcgccctc acccacttaa tgcgctaact gcggacgaaa ttaaacaggc
     2821 cgttgaaatt gttaaagctt ccgcggactt caaacccaat acccgtttta ctgagatctc
     2881 cctgctaccg ccagataaag aagctgtctg ggcgtttgcg ctggaaaaca aaccggttga
     2941 ccagccgcgc aaagccgacg tcattatgct cgacggcaaa catatcatcg aagcggtggt
     3001 ggatctgcaa aacaacaaac tgctctcctg gcaacccatt aaagacgccc acggtatggt
     3061 gttgctggat gatttcgcca gtgtgcagaa cattattaac aacagtgaag aatttgccgc
     3121 tgccgtgaag aaacgcggta ttactgatgc gaaaaaagtg attaccacgc cgctgaccgt
     3181 aggttatttc gatggtaaag atggcctgaa acaagatgcc cggttgctca aagtcatcag
     3241 ctatcttgat gtcggtgatg gcaactactg ggcacatccc atcgaaaacc tggtggcggt
     3301 cgttgattta gaacagaaaa aaatcgttaa gattgaagaa ggtccggtag ttccggtgcc
     3361 aatgaccgca cgcccatttg atggccgtga ccgcgttgct ccggcagtta agcctatgca
     3421 aatcattgag cctgaaggta aaaattacac cattactggc gatatgattc actggcggaa
     3481 ctgggatttt cacctcagca tgaactctcg cgtcgggccg atgatctcca ccgtgactta
     3541 taacgacaat ggcaccaaac gcaaagtcat gtacgaaggt tctctcggcg gcatgattgt
     3601 gccttacggt gatcctgata ttggctggta ctttaaagcg tatctggact ctggtgacta
     3661 cggtatgggc acgctaacct caccaattgc tcgtggtaaa gatgccccgt ctaacgcagt
     3721 gctccttaat gaaaccatcg ccgactacac tggcgtgccg atggagatcc ctcgcgctat
     3781 cgcggtattt gaacgttatg ccgggccgga gtataagcat caggaaatgg gccagcccaa
     3841 cgtcagtacc gaacgccggg agttagtggt gcgctggatc agtacagtgg gtaactatga
     3901 ctacattttt gactggatct tccatgaaaa cggcactatt ggcatcgatg ccggtgctac
     3961 gggcatcgaa gcggtgaaag gtgttaaagc gaaaaccatg cacgatgaga cggcgaaaga
     4021 tgacacgcgc tacggcacgc ttatcgatca caatatcgtg ggtactacac accaacatat
     4081 ttataatttc cgcctcgatc tggatgtaga tggcgagaat aacagcctgg tggcgatgga
     4141 cccagtggta aaaccgaata ctgccggtgg cccacgcacc agtaccatgc aagttaatca
     4201 gtacaacatc ggcaatgaac aggatgccgc acagaaattt gatccgggca cgattcgtct
     4261 gttgagtaac ccgaacaaag agaaccgcat gggcaatcca gtttcctatc aaattattcc
     4321 ttatgcaggt ggtactcacc cggtagcaaa aggtgcccag ttcgcgccgg acgagtggat
     4381 ctatcatcgt ttaagcttta tggacaagca gctttgggta acgcgttatc atcctggcga
     4441 gcgtttcccg gaaggcaaat atccgaaccg ttctactcat gacaccggtc ttggacaata
     4501 cagtaaggat aacgagtcgc tggacaacac cgacgccgtt gtctggatga ccaccggcac
     4561 cacacatgtg gcccgcgccg aagagtggcc gattatgccg accgaatggg tacatactct
     4621 gctgaaacca tggaacttct ttgacgaaac gccaacgcta ggggcgctga agaaagataa
     4681 ttgattgttt cagactaaaa aaacggcacc aggtgccgtt ttttacttat gagcgaacaa
     4741 gattaatacc gtacacacac cgacttagtt tcacaccaac cgtccagcca gtcggggcca
     4801 aaatcacggc ctgttcctga ctgcttcatc ccaccaaacg gtaagttagc gtcaattaag
     4861 gtatgactgt ttacccacac cgtccctgcc tgtaagcgat cgctatattc cagagcctgg
     4921 gagagatttt gcgtccagac actggcagtt aagccatatt ccgtgtcgtt tgccagttgt
     4981 aacgcctctt ctccatccgc tactcgcacc aggttaacca ccggaccaaa cacctcttca
     5041 cgagttaagc gcaatttagc atcgggattt accaccagcg ttggcgcaac ataatacccc
     5101 tctccggctg gtccattcga tccgcgaatc agctctgctt gctgtgcctg cgcatcgtcg
     5161 aggaatgaac acactttgcc gcagtgcgca cgagaaacca aagggttaat ctgtgcaaca
     5221 ggtgacatcc ccggtcccac ttgcaacgat tttaccgcct gctcaaatcc actaaccagc
     5281 gtgtcaaaca acggcgcttc aatataaatt cgcgaactgg cggcgcatac ttgcccttga
     5341 ttcaggaagc ttccggtcat caagccttca ataacccatt gcggatcagc atcttttaat
     5401 acaattgccg ggtttttacc gcccagttcc agcgttacac gcgttaagcg atcagcagca
     5461 gttctggcaa taccttttcc cgtcgcggtt gaaccggtaa aactgatttt cgcaacatga
     5521 ggatgtgacg tcagggccgc gccgcataca gcacctgacc cggtgacgac attaaaaacg
     5581 ccatcaggga taccagcctc gctggccagt tccgccacgc gcaacatcgt cagtggcgtg
     5641 gtttccgaag gcttaatcac gattgaacag cctgctgcca gtgctggcat caccttccac
     5701 atgccaatca tcaacggaaa gttccatggc acaattcccg ctaccacgcc aaccggctct
     5761 ttacgcgtcc aggcctgata acgcgccccc tggggtaagg gaatcgacaa gtccagcgtt
     5821 ttacccgcga ttttggtcgt taacccggcg gtataacgca tccagttcag cgtacagccc
     5881 acttcaaaag cacgggaaat ggcaattgac ttgccttgct ccagggtttc cagttgcgcc
     5941 agctcctcac tgtgctgctc caccagatca gcaaaacgta gcagaatacg ttcacgctct
     6001 gcgggtaatc gcccggccca gcgacgcgag acaaaggccc gccaggcaga catgactgcg
     6061 ttatctacat ccgcttcgtt ggcatcagca gtagacgcaa tttcttgccc ggtggccgga
     6121 tcaaagatcg ccaaccgttt ttcactttgt gcggggccag gacgaccatc aatataaaga
     6181 ccgtgttgac gatcgagaaa ctgttggacc tggcttaata ctgctacatg cggctctgtc
     6241 atcacttttc cttattattt acccagtgtg atttcagtgt acgcagcgac aagaaaagtc
     6301 acttttttcg ctgtgacatt cgctttgtca gctgtgacaa gctccgcaaa tcgtgacaat
     6361 aaccgcttat gtctctatgc ctgacaagac ttatttccta tagtcaggct cattagcttc
     6421 gtttattgca acacaaatgc aacaataaaa atacatttca cagagcgaaa acgtgccatg
     6481 aaccccgcaa tggataatga gtttcagcaa tggctttccc aaatcaatca ggtatgcgga
     6541 aattttaccg gacgcctgct gactgagcgt tacacgggtg tactggacac gcattttgcc
     6601 aaaggactaa agctgagtac cgtgacaacc agcggggtga atttatcccg cacctggcag
     6661 gaagtaaaag gcagcgacga tgcctggttt tacaccgttt ttcagcttag tggtcaggca
     6721 ataatggagc aggatgagcg tcaggtgcag attggcgcag gcgatattac gttactcgat
     6781 gcctcacgcc cctgttcgct ttactggcag gagtcttcta aacagatttc attacttttg
     6841 ccacgcactc tgctggaaca atattttccc catcaaaaac ctgtctgcgc agaaagactg
     6901 gacgctgact tacccatggt gcaactcagt catcgtctgt tacaggaaag catgaataac
     6961 ccggcacttt ctgaaacaga aagtgaagct gcgctacagg cgatggtgtg tctgctgcgc
     7021 ccggtacttc atcagcggga atctgtccaa cctcgccgtg aacgtcagtt tcaaaaagtg
     7081 gttacgttga tagacgataa tattcgcgaa gagatattac gcctggagtg gatagccgca
     7141 gagacgggta tgtcagtacg tagtttgtac cgaatgtttg ccgataaagg tttggtcgtc
     7201 gcgcaatata ttcgtaaccg tcgtctcgat ttttgtgcag atgcgattcg ccatgccgca
     7261 gatgatgaaa aactggcagg catcggcttt cattggggat tttctgacca gagtcatttt
     7321 tcaacggtat ttaagcaacg ctttgggatg acgccaggcg agtatcgacg taaattccgc
     7381 taactaattc gcgttgcatg tactgcaacg ccaattagtt aaaacagata tttgactacg
     7441 catatttcag ggcaaaaggc aatttgacag gagtttttga atattcacct tgtttccgga
     7501 aaatagatta atcgccttct cctgtagcgt tatcccgata ttgcggtttt gctattaaga
     7561 ttttcgcatc catttaccgt ttatcccttg tacatattcc cccggtttgg cccgagctac
     7621 gagcttttgc ccggcgagtt tcgcgatatc atctgcagac acattatttt gctttgccag
     7681 ttgttggtaa ctcgctttac gttcggcatt aatgtcggtt actaatttct ctgtttcagc
     7741 atccgttttc agcgcaacca aataaccata aaatgtttca cctactcgcc cctgagttct
     7801 ggcttcatcc agagtcaatg ccattacatt gctgcttacc agcccaacaa gaaacgcaca
     7861 aagaagtaat gttttcttca tcatcgcctc agaaaagatc gctacgagtt tcaagcagct
     7921 cttcgacatc tttgtctgcc ttgatgatga tctcatgctc aattttaacg ttcatattga
     7981 tagtgatcgg ttccttaggt gcagcgactt caatacgcgg cgtacagcca accagcataa
     8041 aagatgacgt caacgcagcc agtaaaattt tcattgtttt tcctcacact cttttccttg
     8101 cggacagtca tttcccggca gacgtgcgtt ctgctccaac catgcctgga gattatcgcc
     8161 aaagcgtaaa ctgcgccaca gcgtaaaaat attctcttca tgatggtaat taaggtttac
     8221 cgtaccgctt ttaccatcaa cgcgactggt acctgtaatg ttggcctgca tggttaataa
     8281 accgagatta tctaaattaa tatttgtcga tgaacggcta atttccatat agcgcaacca
     8341 gttaattgct gaacccgcag tcatattgtc tttcaccacc gcatctgcgg tgtctttatc
     8401 cagccgcaag gtcattggcc cgctgtttgc cagccaacca tctttcacta tccatttttc
     8461 gttgtttagc cataacggca atgcaccact aaatgccccg gacatggcga attgtttcgg
     8521 attgacggcg ctaaccagtt cgctggatga gagattattc agccgcaaca gcgccgggtc
     8581 atgctgcggc atacgtaatt gtttcatcag tacgttaccg cctaacacat cgacgctaac
     8641 atcagtcaac agcaagggtt cttcttcagt ccacggataa cgcccttgca aatcagccgt
     8701 aatatttttc gctgtcacca gattaatcac ttcggcaatt cgcaacgtaa cggggccgcg
     8761 agtccccaga tgccaggctc catcggcaaa acggaaaggc aggacaaaat cgacaccgtt
     8821 cacctgatta tctggcatcc aggcactacc gcctttcaac acgccgtgcc ctcccgcgcg
     8881 gaatccttgt tcaggcgcag cagaaaatgc aacctgagca tacagttcac catcgcgtaa
     8941 gttcatcttc cagtcgggtg gcaccagcgg ctggaataca gtcagtgatt gtttaggcca
     9001 ccaggcgtta ccgcgcagac gaataccgtc ccagcgaccg tttacccgaa ccgggccaat
     9061 ctcaccagca tgtaaatcgc ctttaaagag gaaataggtc ggatcgcgcc catcgacgct
     9121 aaattttaag gttgatggcg gtaacacact gccgccagtg aacagcgttt gcccggcgtc
     9181 cagtgacagc gcgccgctga aggagggatg ctgtgcgtca cgtacccaga cgacgggctt
     9241 gtcgagaatt aatcgcggct tttctaccgt catcgtaccg tattgtaact ggtcgaaacc
     9301 ggtggagaga tcggtcagcg taatcgtgct gtcatgccac tcaccttttc ctgcgacatc
     9361 ccaggtggca ttcatcggtg taaaactccc ttttccccag tagcgccact gccagcggcc
     9421 agcgtcaggg agaaaatcat tcgccagccc atccatatgc agcacgaaat cgcccagttc
     9481 attttcatgc gcctgcaaga tggcctgcaa acgtccgtca acaccacgtt gggtgacttt
     9541 tacacccgct aaaggccagc ggatttcatc gatgtccagc gaatcgatga ctcttccctt
     9601 cgaacgtaat aacgcgcctg gctcaaaggc cagcgttggg tcagtcagac ttccacttag
     9661 ctgcgcgggt aagcgggcat ataaaatgag gtccgcctgt ttcgcttcac cggttagctg
     9721 cagaggcagc tggctgttat ccatgcttaa ttttcctggg ccaaaattta gcaccgcgtt
     9781 gcctttaccc gcttgcccct gggtcagcac actcagtcgt ccgctgatca gagcgttctc
     9841 aagccctgcc tgccagttgt cgactttgac acccagtcgg ccactcaaag gaaaacctgc
     9901 atacggccaa ctccagcgac catcgcttac ggtcaattgt tgacgagtaa tttgccacgg
     9961 caaatcgagc aacggatcgc cgttatcccg tgccagcaca atcaattgcc cgctattttc
    10021 ctgccagtcc agctctgcat ccaccagtga cggttcctgc ggcaagttta acgtcgcagt
    10081 agcatgccca cttacaggaa gtccatccgg cacgagcggc atagtaaatt cccccaccag
    10141 tttaaccggc ggctgatttt caaacgcgac gacatccagt tcgctgactg taagttgttg
    10201 ccctttcagc tggccttgaa atttaacttt ttcgccctga taacgcagtt gctggatatc
    10261 agaggttaat gcgagagaga gttttccctg ccattcctgc cagggagaaa aaatcagttt
    10321 atcgatattg atccaggtgt taggcagcat ggactgccac tgcgcgaggg tttttggagc
    10381 agctggcgat tgctccgtct gcggcaattt cgccagacaa gcagaatcaa gttctaccat
    10441 gccgacgttc aataaccagc ggctgggatg tgaaaggctg gcgttggtga tatgcgcaag
    10501 ctgacaatct cccaccagat aacggagatc gggaatgatt aaacctttac gcgtaatgcg
    10561 tgggctttca tctaatgcaa tacgtgtccc gagcggtagc cagatgcccg ccagcgtggg
    10621 aacccacagc ccgagcgtca tcagcagcgt caacggcaca agaataatca gtaataacag
    10681 cgcgagaacg gctttatatt tacccagcat gggtagttaa tatcctgatt tagcgaaaaa
    10741 ttaagcattc aatacgggta ttgtggcatg tttaaccgtt cagttgaagg ttgcgcctac
    10801 actaagaata gttgttgatg aatttttcaa tatcgccata gctttcaatt atatttgaaa
    10861 ttttgtaaaa tatttttagt agcttaaatg tgattcaaca tcactggaga aagtcttatg
    10921 aaactcgccg tttatagcac aaaacagtac gacaagaagt acctgcaaca ggtgaacgag
    10981 tcctttggct ttgagctgga attttttgac tttctgctga cggaaaaaac cgctaaaact
    11041 gccaatggct gcgaagcggt atgtattttc gtaaacgatg acggcagccg cccggtgctg
    11101 gaagagctga aaaagcacgg cgttaaatat atcgccctgc gctgtgccgg tttcaataac
    11161 gtcgaccttg acgcggcaaa agaactgggg ctgaaagtag tccgtgttcc agcctatgat
    11221 ccagaggccg ttgctgaaca cgccatcggt atgatgatga cgctgaaccg ccgtattcac
    11281 cgcgcgtatc agcgtacccg tgacgctaac ttctctctgg aaggtctgac cggctttact
    11341 atgtatggca aaacggcagg cgttatcggt accggtaaaa tcggtgtggc gatgctgcgc
    11401 attctgaaag gttttggtat gcgtctgctg gcgttcgatc cgtatccaag tgcagcggcg
    11461 ctggaactcg gtgtggagta tgtcgatctg ccaaccctgt tctctgaatc agacgttatc
    11521 tctctgcact gcccgctgac accggaaaac taccatctgt tgaacgaagc cgccttcgat
    11581 cagatgaaaa atggcgtgat gatcgtcaat accagtcgcg gtgcattgat tgattctcag
    11641 gcagcaattg aagcgctgaa aaatcagaaa attggttcgt tgggtatgga cgtgtatgag
    11701 aacgaacgcg atctgttctt tgaagataaa tccaacgacg tgatccagga tgacgtattc
    11761 cgtcgcctgt ctgcctgcca caacgtgctg tttaccgggc accaggcatt cctgacagca
    11821 gaagctctga ccagtatttc tcagactacg ctgcaaaact taagcaatct ggaaaaaggc
    11881 gaaacctgcc cgaacgaact ggtttaatct tgccgctccc ctgcaaccca ggggagctga
    11941 ttcagataat ccccaatgac ctttcattct ctattcttaa aatagtcctg agtcagaaac
    12001 tgtaattgag aaccacaatg aagaaagtag ccgcgtttgt tgcgctaagc ctgctgatgg
    12061 cgggatgtgt aagtaatgac aaaattgctg ttacgccaga acagctacag catcatcgct
    12121 ttgtgctgga aagcgtaaac ggtaagcccg tgaccagcga taaaaatccg ccagaaatca
    12181 gctttggtga aaaaatgatg atttccggca gcatgtgtaa ccgctttagc ggtgaaggca
    12241 aactgtctaa tggtgaactg acagccaaag ggctggcaat gacccgtatg atgtgcgcta
    12301 acccgcagct taatgaactc gataacacca ttagcgaaat gctgaaagaa ggtgcacaag
    12361 tggatctgac cgcgaaccag ttaacgctgg cgaccgcgaa acagacatta acttataagc
    12421 tggcggattt aatgaattaa tagctgccac agctcccggc ggcaagtgac tgttcgctac
    12481 agcgtttgcc gttgggtaat gcacacatcc caatcgccgt accatccagt tgacgggcaa
    12541 cagaaagcga accgccgatc attgcacaat ttgcttctcc actactggac atcgacgctt
    12601 ttaaacctgg cgctacgtgc gccgcagtcg cctgctgaac aggctcacta ctacacgccg
    12661 acaacaataa agcggcacac cctacccaaa acgctgctcg catctcttct tcctctgatc
    12721 ttcaagccaa acgacaccgc cataaataat aggcagcaca gagggcgacg tcgagagctg
    12781 tcctgcgcgt tgccccgcat ttttactttt ttatggctat ttttttgccc tctgtttgat
    12841 caaaacattc attacgctga tgtgggggac acaaaagcga aaatacagaa gaaagccatt
    12901 tgctaaaatt gaaagattac tactgggcgc gcagcaattt cgtgcgcccc tcatttgcgc
    12961 aatgtaaggg tgtcatatga ttactattga cggtaatggc gcggttgctt cggtggcgtt
    13021 tcgcaccagt gaagttatcg ccatctaccc tattaccccc agttccacga tggcagaaca
    13081 ggctgatgcc tgggccggaa acggcttgaa gaacgtttgg ggagacacac cacgcgtggt
    13141 tgaaatgcag tcggaagcgg gtgctatcgc taccgtgcat ggcgctttgc agacgggtgc
    13201 cctttcaaca tcgtttacgt catcgcaggg tttgctgctg atgatcccga cgctgtacaa
    13261 actggcaggc gaactcacac cgtttgtcct gcatgtagcg gcacgtaccg ttgccacaca
    13321 tgcactctct atttttggcg atcattccga cgtcatggcg gtgcgccaga cgggttgcgc
    13381 gatgttgtgt gcggcaaacg tccaggaagc gcaagacttt gctctcattt cgcacatcgc
    13441 gacgctgaaa agccgcgtgc catttattca tttctttgat ggtttccgca cgtcccacga
    13501 aatcaataaa attgtcccgc tggccgatga cactattctt gatctcatgc cgcaggccga
    13561 aattgatgct catcgcgccc gggcgctcaa cccggaacat ccggtgatcc gcggtacgtc
    13621 cgccaatcct gacacttatt tccagtctcg cgaagccacc aacccctggt acaacgcggt
    13681 ctatgaccat gttgaacagg cgatgaatga tttcgctgcc gcgacaggtc gtcagtatca
    13741 gccgtttgag tattacgggc atccgcaagc ggaacgggtg attatcctga tgggctctgc
    13801 cattggcacc tgtgaagaag tgattgatga attgctaacc cgtggcgaaa atgtcggcgt
    13861 gctgaaagtt cgcctgtacc gccccttctc cgctaaacat ttactgcaag ctctgccggg
    13921 atccgtacgc agcgtggcgg tactggacag aaccaaagaa cccggtgccc aggcagaacc
    13981 gctctatctg gatgtgatga ccgcactggc agaagccttt aataatggcg agcgcgaaac
    14041 tctgccccgt gtcattggtg ggcgctatgg tctttcatcc aaagaatttg gcccggactg
    14101 tgtactggcg gtatttgccg agctcaacgc ggctaaaccg aaagcgcgct ttacggttgg
    14161 tatttacgat gatgtgacta atctgtcact gccgttgccg gaaaacaccc tgccaaactc
    14221 ggcgaaactg gaagccttgt tttatggcct tggtagtgat ggcagcgttt ccgcgaccaa
    14281 aaacaatatc aagattatcg gtaattccac gccgtggtac gcacagggct attttgttta
    14341 cgactccaaa aaggcgggcg gcctgacggt ttctcacctt cgagtgagcg aacagccgat
    14401 tcgttccgct tatctcattt cccaggctga ttttgttggc tgccaccagt tgcagtttat
    14461 cgataaatat cagatggctg agcgtttaaa acctggcggc attttcctgc tcaacacacc
    14521 gtacagcgca gatgaagtgt ggtcgcgctt gccgcaagaa gttcaggccg tgttaaacca
    14581 gaaaaaagcg cgcttctatg tgattaacgc ggcgaaaatc gcccgcgaat gtggcctggc
    14641 ggcccgtatt aataccgtca tgcagatggc ttttttccat ctgacgcaaa ttctgcctgg
    14701 cgatagcgcc ctcgcagaat tgcagggtgc gattgccaaa agttacagta gcaaaggcca
    14761 ggatctggtg gaacgcaact ggcaggctct ggcgctggcg cgtgaatccg tagaagaagt
    14821 tccgttgcaa ccggtaaatc cgcacagcgc caatcgaccg ccagtggttt ccgatgccgc
    14881 ccctgatttc gtgaaaaccg taaccgctgc gatgctcgcc gggcttggtg acgccctccc
    14941 cgtttcggcg ctgccgccag acggcacctg gccgatgggc actacgcgct gggaaaaacg
    15001 caatatcgcc gaagagatcc ccatctggaa agaggaactc tgtacccaat gtaaccactg
    15061 cgttgccgct tgcccacact cagctattcg cgcaaaagtg gtgccgcctg aagcgatgga
    15121 aaacgcccct gccagcctgc attcgctgga tgtgaaatcg cgtgatatgc gcgggcagaa
    15181 atatgtcttg caggtggcac cggaagattg caccggttgt aacctgtgcg tcgaagtttg
    15241 cccggcgaaa gaccgtcaga atccagagat taaagccatc aatatgatgt ctcgcctgga
    15301 acatgtcgaa gaagagaaaa tcaattacga tttcttcctc aacctgccag aaatcgaccg
    15361 tagcaaactg gaacgtattg atattcgtac atcgcagctg attacaccgc tgtttgaata
    15421 ttcaggtgct tgctccggtt gtggcgagac gccgtatatt aaattactga ctcagctcta
    15481 tggcgaccgg atgttgatcg ctaacgccac tggctgttct tcaatttatg gcggtaacct
    15541 gccctctaca ccgtatacca ccgatgccaa cggtcgtggg ccggcatggg cgaactctct
    15601 atttgaagat aatgccgaat ttggccttgg tttccgcctg acggtcgatc aacaccgtgt
    15661 ccgcgtgctg cgtctgctgg atcaatttgc cgataaaatc ccggcggaat tactgacggc
    15721 gttgaaatca gacgccacgc cagaggttcg tcgtgaacag gttgcagctt tacgccagca
    15781 actcaacgat gttgccgaag cacatgaact gctacgtgat gcagatgcac tggtggaaaa
    15841 atcaatctgg ctgattggtg gtgatggctg ggcttacgat atcggctttg gcggtctgga
    15901 tcatgtattg agtttgacgg aaaacgtcaa cattctggtg ctggatacgc aatgctattc
    15961 caacaccggt ggtcaggcgt cgaaagcgac accgctgggt gcagtaacta aatttggcga
    16021 gcacggcaaa cgtaaagcgc gtaaagatct tggcgtcagt atgatgatgt acggtcatgt
    16081 ttatgtggcg cagatttctc tcggcgcgca gctgaaccag acggtgaaag cgattcagga
    16141 agcggaagcg tatccggggc catcgctgat cattgcttat agcccgtgtg aagagcatgg
    16201 ttacgatctg gcactcagcc acgaccagat gcgccaactc acagctaccg gcttctggcc
    16261 gctatatcgc tttgatccgc gtcgtgccga tgaaggcaaa ctgccgctgg ccttggattc
    16321 acgcccgccg tcagaagcac tggaagaaac gttacttcac gagcaacgtt tccgtcggct
    16381 gaattcgcag cagccagaag tggcagaaca gttatggaaa gatgctgcag ctgatttgca
    16441 aaaacgctat gacttcctgg cacaaatggc cggaaaagcg gaaaaaagca acaccgatta
    16501 aatgctctgg ataaggatta tccaattcta aaaaaaaagc ccggacgact gttcgggctt
    16561 gtctttttat atgttggaaa atcagtggca atgcaatggc ccaacagaaa ataaaatctg
    16621 tgacaataaa ggcatat
//
LOCUS       DAPHBJ010000093        16456 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.094, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000093 DAPHBJ010000000
VERSION     DAPHBJ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.094"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(29..1483)
                     /gene="xylB"
                     /locus_tag="RHZ76_002677"
     CDS             complement(29..1483)
                     /gene="xylB"
                     /locus_tag="RHZ76_002677"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDV2813292.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1555..2877)
                     /gene="xylA"
                     /locus_tag="RHZ76_002678"
     CDS             complement(1555..2877)
                     /gene="xylA"
                     /locus_tag="RHZ76_002678"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDV2813293.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3243..4235
                     /gene="xylF"
                     /locus_tag="RHZ76_002679"
     CDS             3243..4235
                     /gene="xylF"
                     /locus_tag="RHZ76_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV2813294.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPHGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            4313..5854
                     /gene="xylG"
                     /locus_tag="RHZ76_002680"
     CDS             4313..5854
                     /gene="xylG"
                     /locus_tag="RHZ76_002680"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HDV2813295.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5832..7013
                     /gene="xylH"
                     /locus_tag="RHZ76_002681"
     CDS             5832..7013
                     /gene="xylH"
                     /locus_tag="RHZ76_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HDV2813296.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7091..8269
                     /gene="xylR"
                     /locus_tag="RHZ76_002682"
     CDS             7091..8269
                     /gene="xylR"
                     /locus_tag="RHZ76_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HDV2813297.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8465..9289)
                     /locus_tag="RHZ76_002683"
     CDS             complement(8465..9289)
                     /locus_tag="RHZ76_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HDV2813298.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9609..11639
                     /gene="malS"
                     /locus_tag="RHZ76_002684"
     CDS             9609..11639
                     /gene="malS"
                     /locus_tag="RHZ76_002684"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDV2813299.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASLVRECPKWDGLPLTVDVSATFPEGAAVRDYYSQQIAIVKNGQIM
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKSLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKMDTHAKAIDGYTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLLLKQGYGFVREHGDDKVLVVWAGQQ"
     gene            11817..13070
                     /gene="avtA"
                     /locus_tag="RHZ76_002685"
     CDS             11817..13070
                     /gene="avtA"
                     /locus_tag="RHZ76_002685"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HDV2813300.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13221..13694)
                     /locus_tag="RHZ76_002686"
     CDS             complement(13221..13694)
                     /locus_tag="RHZ76_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDV2813301.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREQGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13796..14644)
                     /gene="yiaJ"
                     /locus_tag="RHZ76_002687"
     CDS             complement(13796..14644)
                     /gene="yiaJ"
                     /locus_tag="RHZ76_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HDV2813302.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            14845..15843
                     /gene="yiaK"
                     /locus_tag="RHZ76_002688"
     CDS             14845..15843
                     /gene="yiaK"
                     /locus_tag="RHZ76_002688"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HDV2813303.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTSAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            15855..16322
                     /locus_tag="RHZ76_002689"
     CDS             15855..16322
                     /locus_tag="RHZ76_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDV2813304.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKVLDFLRATDFNALEPGVVEIDGKN
                     IYTQIIDLTTREAVVNRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRNI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCIMQTASEIRKIVVKVALTALN"
BASE COUNT         4181 a         4178 c         4053 g         4044 t
ORIGIN      
        1 ccccacccgg tcaggcaggg gataacgttt acgccattaa tggcagaagt tgctgataga
       61 ggcgacggaa cgtttctcgt cgtggctgat aagcggcata acgctgcgca tctggtagat
      121 gcgactgttc taacggtagt tgcggcaaca attcaatgag cgatttctct ggattcgccg
      181 cgatctgcgc cagccttgct gcgcccagtg ctggccccac atcccccccc gtacggtaat
      241 cgagctgctg accgctgata tccgccagca tctgacgcca gtactcacta cgcgccccgc
      301 ccccaatcaa cgtaacactt tgcggtttaa taccgcaggc atgcacgaca tccatgccat
      361 ctgccagcgc ataacccacg ccttccagca ctgctcgcgc cagttcattg gggccatgtt
      421 gatgagtcaa accaaagaaa acccccttcg cctggggatt attgtgtggc gtacgctcgc
      481 cggaaagata aggcagaaac caaactggct cggcactttc atcagcctgt tgagctgcag
      541 cgattaaagc tgggacattg ctcaggccgg ttaatttcgc ggcccaatcc agacacgacg
      601 ctgcactcag catcacagac attaaatgcc aacgttgcgg tagcgcatgg caaaagctat
      661 gtacggcgct ttctggcttg cttaagaacc cttcgctgac agcaaaatag acccccgacg
      721 tccccagcga taacattgcc tgattagcat caaccattcc cacaccaact gcaccagctg
      781 cattgtcgcc accgcctgcg acaactggca ccgtcgccat accccacgct ttcgcaactt
      841 caggtaacaa agcaccagta atttcgctgc cttcgtataa tgcgggcatc tggtcacgag
      901 ataagtcgca agcctgcagc atgacgtcac tccagtcacg ctttgcgaca tccagccaca
      961 tggtgccagc tgcgtcagac atatcgctgg caaactcccc cgtcatacgc agacgcaagt
     1021 aatctttcgg taataatact ttgtcgattt gacggaatat ctccggctca tgccgctgaa
     1081 cccatagcaa tttaggcgca gtaaatccgg gcatcatcag gttgccggta atcactcgtg
     1141 attgcggaac tctcgcttcc agcaaagtgc actcttgcgc acagcgcccg tcgttccaca
     1201 aaatggcagg gcgcaatacc cgttgttgag catccagtaa ggttgctcca tgcatctggc
     1261 cggcaatacc caatgcttta acgtcctgca gagaatgctg atcgcccaga gctttcattg
     1321 cgcgatcagt tgcctgccac cactgttccg ggtcttgttc cgaccagagt ggatgcgggc
     1381 gcgaaacggt cagcttttcc gtttgcgaag caaccacctc accctgctcg ttgagcaaaa
     1441 taacttttac gcccgaggtg ccaagatcta tcccgatata catatcgatc gttccttaaa
     1501 aaaatgcccg gtatcgctac cgataaccgg gccaacggac tgcacagtta gccgttattt
     1561 gtcgaacaga taatgattta ccagattttc cagttgctcc tggcgaccac tctgatgcac
     1621 cggagacaaa ttatgttcct gagcatattt ggctaaatct gccagtgaca tttggccttt
     1681 caggatttgc tggcccaatt cgctattcca gccggaataa cgctgcgcga tgcgtttatc
     1741 cagctcgcca tcttcaatca tgcgcgctgc aattttcagc gccagtgcca tcgtatccat
     1801 cgcgccgata tgaccgtaaa acagatcata tttatcagta ctttgacgac gtactttggc
     1861 atcgaagttc agaccaccgg tggtgaaacc gcctgctttg agaatttcat acatcaccag
     1921 cgcattctct tccacactgt tcgggaactg gtcggtgtcc cagcccagtt gcgcatcgcc
     1981 acggttggcg tcgacagaac cgaacaggcc aagcgcaatg gcggtggcta tttcatgatg
     2041 gaaagagtga cctgccagcg tcgcgtggtt agcttcaatg ttcagtttaa tctctttttc
     2101 cagaccaaac tgtttcagga agccatagac cgtcgcggca tcgtaatcat attgatgttt
     2161 ggtcggttct tgcggtttcg gttcgataag caacgtgccc tggaaaccga ttttatgttt
     2221 atgctcaacc accatctgca taaagcggcc cagttgttca cgctcctgac gcaagtcggt
     2281 atttaacagc gtttcgtaac cttcacgacc gccccacagg acatagtttt caccgcccaa
     2341 tttatgggtt gcttccatcg ctgtaacaac ttgcgttgcc gcccagctga agacttcagg
     2401 atctgggttc gtcgccgcac ccgcgccgta gcgagggttt gtaaagcagt tggcggttcc
     2461 ccacagcagc ttcacgccgc tctcttcttg cttgcctgcc aggacatcaa ccatttgcgc
     2521 aaaattattg atgtactctt ttaacgacgc gccctcaggg gaaacatcca catcgtggaa
     2581 gcaataaaat ggcacatgta acttgtggaa aaactcaaat gcgacatctg ctttacgctt
     2641 cgccaacgcc agtgcctcac caggctgctg ccacggacga ttaaacgccc ccacaccaaa
     2701 catatccgcc ccgttccagc agaaggtgtg ccagtagcag gcggcaaaac gcaagtgctc
     2761 ttccatacgc ttacccaaca ccagttcgtc gggattgtag tgacggaatg ctaacgggtt
     2821 tgaggatttt gagccttcat aacgaacgcg atcgagctgg tcaaaatagg cttgcatatt
     2881 gaactccata atcaggtaat gccgcgggtg atggatgatg tcgtaatatt gggcactccc
     2941 tttcagttgc tcaattatgt tatttcacac tgctattgag ataattcaca agtgtgcgct
     3001 cgctcgcaaa ataaaatgga atgatgaaac tgggtaattc ctcgaagaga aaaatgcaat
     3061 aagtacaatt gcgcaacaaa agtaagatct cggtcataaa tcaagaaata aaccaaaaat
     3121 cgtaatcgaa agataaaaat ctgtaattgt tttcccctgt ttagttgcta aaaattggtt
     3181 acgtttatcg cggtgattgt tacttattaa aactgtcctc taactacaga aggccctaca
     3241 ccatgaaaat aaagaacatt ctactcaccc tttgcacctc actcctgctt accaacgttg
     3301 ctgcacacgc caaagaagtc aaaataggta tggcgattga tgatctccgt cttgaacgct
     3361 ggcaaaaaga tcgagatatc tttgtgaaaa aggcagaatc tctcggcgcg aaagtatttg
     3421 tacagtctgc aaatggcaat gaagaaacac aaatgtcgca gattgaaaac atgataaacc
     3481 ggggtgtcga tgttcttgtc attattccgt ataacggtca ggtattaagt aacgttgtaa
     3541 aagaagccaa acaagaaggc attaaagtat tagcttacga ccgtatgatt aacgatgcgg
     3601 atatcgattt ttatatttct ttcgataacg aaaaagtcgg tgaactgcag gcaaaagccc
     3661 tggtcgatat tgttccgcac ggtaattact tcctgatggg cggctcgccg gtagataaca
     3721 acgccaagct gttccgcgcc ggacaaatga aagtgttaaa accttacgtt gattccggaa
     3781 aaattaaagt cgttggtgac caatgggttg atggctggtt accggaaaac gcattgaaaa
     3841 ttatggaaaa cgcgctaacc gccaataata acaaaattga tgctgtagtt gcctcaaacg
     3901 atgccaccgc aggtggggca attcaggcat taagcgcgca aggtttatca gggaaagtag
     3961 caatctccgg ccaggatgcg gatctcgcag gtattaaacg tattgctgcc ggtacgcaaa
     4021 ctatgacggt gtataaacct attacgttgt tggcaaatac tgccgcagaa attgccgttg
     4081 aattgggcaa tggtcaggag ccaaaagcag atacctcact gaataatggc ctgaaagatg
     4141 tcccctcccg cctcctgaca ccgatcgatg tgaataaaaa caacatcaaa gatacggtaa
     4201 ttaaagacgg attccacaaa gagagcgagc tgtaagcgtt acgccccggc gcggagcggg
     4261 ggcgtgattt ctctccatgc cgcgtgaatg aattggctta ggtggagtcg ttatgcctta
     4321 tctacttgaa atgaagaaca ttaccaaaac cttcggcagt gtgaaggcga ttgataacgt
     4381 ctgcttgcgg ttgaatgctg gcgaaatcgt ctcactttgt ggggaaaatg ggtctggtaa
     4441 atcaacgctg atgaaagtgc tgtgtggtat ttatccccat ggctcctacg aaggcgaaat
     4501 tatttttgcg ggagaagaga ttcaggcgag tcacatccgc gataccgaac gcaaaggtat
     4561 cgccatcatt catcaggaat tggccctggt gaaagaattg accgtgctgg aaaatatctt
     4621 cctgggtaac gaaataaccc acaatggcat tatggattat gacctgatga cgctacgctg
     4681 tcagaagctg ctcgcacagg tcagtttatc catttcacct gatacccgcg ttggcgattt
     4741 agggcttggg caacaacaac tggttgaaat tgccaaggca cttaataaac aggtgcgctt
     4801 gttaattctc gatgaaccaa cagcctcatt aactgagcag gaaacgtcgg ttttactgga
     4861 tattattcgc gatctacaac agcacggtat cgcctgtatt tatatttcgc acaaactcaa
     4921 cgaagtcaaa gcgatttccg atacgatttg cgttattcgc gacggacagc acattggtac
     4981 gcgtgatgct gccggaatga gtgaagacga tattatcacc atgatggtcg ggcgagagtt
     5041 aaccgcgctt taccctaatg aaccacatac caccggagat gaaatattac gtattgaaca
     5101 tctgacggca tggcatccgg tcaatcgtca tattaaacga gttaatgatg tctcgttttc
     5161 cctgaaacgt ggcgaaatac tgggtattgc cggactcgtt ggtgccggac gtaccgagac
     5221 cattcagtgc ctgtttggcg tgtggcccgg acaatgggaa ggaaaaattt atattgatgg
     5281 caaacaggta gatattcgta actgtcagca agccatcgcc caggggattg cgatggtacc
     5341 cgaagacaga aagcgcgacg gcatcgttcc ggtaatggcg gttggtaaaa atattaccct
     5401 cgccgcactc aataaattta ccggtggcat tagccagctt gatgacgcgg cagagcaaaa
     5461 atgtattctg gaatcaatcc agcaactcaa agttaaaacg tcgtcccccg accttgctat
     5521 tggacgtttg agcggcggca atcagcaaaa agcgatcctc gctcgctgtc tgttacttaa
     5581 cccgcgcatt ctcattcttg atgaacccac caggggtatc gatattggcg cgaaatacga
     5641 gatctacaaa ttaattaacc aactcgtcca gcagggtatt gccgttattg tcatctcttc
     5701 cgaattacct gaagtgctcg gccttagcga tcgtgtactg gtgatgcatg aagggaaact
     5761 aaaagccaac ctgataaatc ataacctgac tcaggagcag gtgatggaag ccgcattgag
     5821 gagcgaacat catgtcgaaa agcaatccgt ctgaagtgaa attggccgta ccgacatccg
     5881 gtggcttctc cgggctgaaa tcactgaatt tgcaggtctt cgtgatgatt gcagctatca
     5941 tcgcaatcat gctgttcttt acctggacca ccgatggtgc ctacttaagc gcccgtaacg
     6001 tctccaacct gttacgccag accgcgatta ccggcatcct cgcggtagga atggtgttcg
     6061 tcataatttc tgctgaaatc gacctttccg tcggctcaat gatggggcta ttaggtggcg
     6121 tcgcggcgat ttgtgacgtc tggttaggct ggcctttgcc acttaccatc attgtgacgt
     6181 tggttctggg actgcttctc ggtgcctgga acggatggtg ggtcgcgtac cgcaaagtcc
     6241 cttcatttat tgtcaccctc gcgggcatgt tggcatttcg cggcatactc attggcatca
     6301 ccaacggcac gactgtttcc cccaccagcg ccgcgatgtc acaaattggg caaagctatc
     6361 tacctgccag cactggcttc atcattggcg cgcttggctt aatggctttt gttggttggc
     6421 aatggcgcgg aagaatgcgc cgtcaggctt tgggtttgca gtctccggcc tctaccgcag
     6481 tagtcggtcg ccaggcttta accgctatca tcgtattagg cgcaatctgg ctgttgaatg
     6541 attaccgtgg cgttcccact cctgttctgc tgctcacgtt gctgttactc ggcggaatgt
     6601 ttatggcaac gcggacggca tttggacgac gcatttatgc catcggcggc aatctggaag
     6661 cagcacgtct ctccgggatt aacgttgaac gcaccaaact tgccgtgttc gcgattaacg
     6721 gattaatggt agccatcgcc ggattaatcc ttagttctcg acttggcgct ggttcacctt
     6781 ctgcgggaaa tatcgccgaa ctggacgcaa ttgcagcatg cgtgattggc ggcaccagcc
     6841 tggctggcgg tgtgggaagc gttgccggag cagtaatggg ggcatttatc atggcttcac
     6901 tggataacgg catgagtatg atggatgtac cgaccttctg gcagtatatc gttaaaggtg
     6961 cgattctgtt gctggcagta tggatggact ccgcaaccaa acgccgttct tgattttgat
     7021 aaaaattttc tcaaagccgg ttacgtatta ccggttttga gtttttgcat gattcagcag
     7081 gaaaagaacc atgtttacta aacgtcaccg catcacatta ctgttcaatg ccaataaagc
     7141 ctatgaccgg caggtagtag aaggcgtagg ggaatattta caggcgtcac aatcggaatg
     7201 ggatattttc attgaagaag atttccgcgc ccgcattgat aaaatcaagg actggttagg
     7261 agatggcgtc attgccgact tcgacgacaa acagatcgag caagcgctgg ctgatgtcga
     7321 cgtccccatt gttggggttg gcggctcgta tcaccttgca gaaagttacc cacccgttca
     7381 ttacattgcc accgataact atgcgctggt tgaaagcgca tttttgcatt taaaagagaa
     7441 aggcgttaac cgctttgctt tttatggtct tccggaatca agcggcaaac gttgggccac
     7501 tgagcgcgaa tatgcatttc gtcagcttgt cgccgaagaa aagtatcgcg gagtggttta
     7561 tcaggggtta gaaaccgcgc cagagaactg gcaacacgcg caaaatcggc tggcagactg
     7621 gctacaaacg ctaccaccgc aaaccgggat tattgccgtt actgacgccc gagcgcggca
     7681 tattctgcaa gtatgtgaac atctacatat tcccgtaccg gaaaaattat gcgtgattgg
     7741 catcgataac gaagaactga cccgctatct gtcgcgtgtc gccctttctt cggtcgctca
     7801 gggcgcgcgg caaatgggct atcaggcggc aaaactgttg catcgattat tagataaaga
     7861 agaaatgccg ctacagcgaa ttttggtccc accagttcgc gtcattgaac ggcgctcaac
     7921 agattatcgc tcgctgaccg atcccgccgt tattcaggcc atgcattaca ttcgtaatca
     7981 cgcctgtaaa gggattaaag tggatcaggt actggatgcg gtcgggatct cgcgctccaa
     8041 tcttgagaag cgttttaaag aagaggtggg tgaaaccatc catgccatga ttcatgccga
     8101 gaagctggag aaagcgcgca gtctgctgat ttcaaccacc ttgtcgatca atgagatatc
     8161 gcaaatgtgc ggttatccat cgctgcaata tttctactct gtttttaaaa aagcatatga
     8221 cacgacgcca aaagagtatc gcgatgtaaa tagcgaggtc atgttgtagc gatgatgaga
     8281 attgtcggcg tcacatcagg taacgctgcg tggttgtcgg atgcggcgtg aacgccttat
     8341 ccgacctacc cgccaggcat gataaaacgc accaacaacg cttcaggctc gtagctcaac
     8401 tgcctgagac aaagtaaaaa gccttatccg actgacaagt cggataaggc tcaaggaaat
     8461 gcaattacat atgcgccgcg attaaccgtt ggttatcctg gtacattgcg aacaggtagt
     8521 tgttataact cttccctttg gtcgagtagc ccttcagctt gtgaatcatc gctgtggcag
     8581 tcacttcctg atccgcttta cgcagctgcg cacgcgattt acggaacgaa gagtaagccg
     8641 ggtgcgtatt caggttagtg acataggcgc tcaccgattc tttgacagaa ctaaactgtg
     8701 agtacccttt cactttacct ggcgcattgg tacaacgtcc tttcatgcat ttcatgccga
     8761 acaggttgtt gttgttgcgc gccagcttcg acgttcccca accgctttct gctgcagcca
     8821 tcgtcgccac catactggtg gggataatgt ctacgcgttc aagcaaggta ttccacggga
     8881 tttttcgcgt attaccggac cacttcacct tgtagcgttt ggcgatgtct ttcagacgcg
     8941 cacgctcagc aggtgaccat tggccctgat actgttttga aatgagccag ttacgttccg
     9001 cagtaatggc cgcattttgg ctggtaatgt aaggcattac ggtccggaga aacgcctttt
     9061 tccttggtgt tccggaaggg tattttcgca aatcaggaag tgaactactc tttgcactat
     9121 tgcgagaata ctcttgttta ctgcttacct gtttattact agctttagtt aagtgggact
     9181 tttgactcgc tgttgttgtg tgcgtcttcg ctaacacctc actcgaaaac accagagtga
     9241 gtaacataag aatcatcgcc ccatatcgtc gtatgggagt caaaatcatc aggtctcctg
     9301 gtcggattta atcattccaa caccttatat ttttcacaaa tttgagagtt gaatctcaaa
     9361 tcatatcaaa aatagctgtc aagagcaccc caaggaatag tccaaatctg aaactatgtc
     9421 acgtgttaac gattcagatt ggcgctaaat cgcagaaaat gtgggggtta tcgcaaaatt
     9481 cagccgtttt ttgcgcgaga tcgctcaccc ttgcttctca tcctgtggac ttaccgctca
     9541 gggatgagtt ttgtttggct tatcgctggc aaactgtctg aaatcgcagc aataaggact
     9601 catccgccat gaaactcgcc gcctgttttc tgacactcct tcctggcttc gccgttgccg
     9661 ccagctggac ttctccgggg tttcccgcct ttagcgaaca ggggacagga acatttgtca
     9721 gccacgcgca gttgcccaaa ggtacgcgtc cactaacgct aaattttgac caacagtgct
     9781 ggcagcctgc ggatgcgata aaactcaatc agatgctttc cctgcaacct tgtagcaaca
     9841 cgccgcctca atggcgattg ttcagggacg gcgaatatac gctgcaaata gacacccgct
     9901 ccggtacgcc aacattgatg atttccatcc agaacgccgc cgaaccggta gcaagcctag
     9961 tccgtgaatg cccgaaatgg gatggattac cgctcacagt ggatgtcagc gccactttcc
    10021 cggaaggagc cgccgtacgg gattattaca gccagcaaat tgcgatagtg aagaacggtc
    10081 aaataatgtt acaacccgct gccaccagca acggtttact cctgctggaa cgggcagaaa
    10141 ctgacacatc cgcccctttc gactggcata acgccacggt ttactttgtg ctgacagatc
    10201 gtttcgaaaa cggcgatccc agtaatgacc agagttacgg acgtcataaa gacggtatgg
    10261 cggaaattgg cacttttcac ggcggcgatt tacgcggcct gaccaacaaa ctggattacc
    10321 tccagcagtt gggcgttaat gctttatgga taagcgcccc atttgagcaa attcacggct
    10381 gggtcggcgg cggtacaaaa ggcgatttcc cgcattatgc ctaccacggt tattacacac
    10441 aggactggac gaatcttgat gccaatatgg gcaacgaagc cgatctacgg acgctggttg
    10501 atagcgcaca tcagcgcggt attcgtattc tctttgatgt cgtgatgaac cacaccggct
    10561 atgccacgct ggcggatatg caggagtatc agtttggcgc gttatatctt tctggtgacg
    10621 aagtgaaaaa atcgctgggt gaacgctgga gcgactggaa acctgccgcc gggcaaacct
    10681 ggcatagctt taacgattac attaatttca gcgacaaaac aggctgggat aaatggtggg
    10741 gaaaaaactg gatcagaacg gatatcggcg attacgacaa tcctggattc gacgatctca
    10801 ctatgtcgct agcctttttg ccggatatca aaaccgaatc aactaccgct tctggtctgc
    10861 cggtgttcta taaaaacaaa atggataccc acgccaaagc cattgacggc tatacgccgc
    10921 gcgattactt aacccactgg ttaagtcagt gggtccgcga ctatgggatt gatggttttc
    10981 gggtcgatac cgccaaacat gttgagttgc ccgcctggca gcaactgaaa accgaagcca
    11041 gcgccgcgct tcgcgaatgg aaaaaagcta accccgacaa agcattagat gataaacctt
    11101 tctggatgac cggtgaagcc tggggccacg gcgtgatgca aagtgactac tatcgccacg
    11161 gcttcgatgc gatgatcaat ttcgattatc aggagcaggc ggcgaaagca gtcgactgtc
    11221 tggcgcagat ggatacgacc tggcagcaaa tggcggagaa attgcagggt ttcaacgtgt
    11281 tgagctacct ctcgtcgcat gatacccgcc tgttccgtga agggggcgac aaagcagcag
    11341 agttattact attagcgcca ggcgcggtac aaatctttta tggtgatgaa tcctcgcgtc
    11401 cgttcggtcc tacaggttct gatccgctgc aaggtacacg ttcggatatg aactggcagg
    11461 atgttagcgg taaatctgcc gccagcgtcg cgcactggca gaaaatcagc cagttccgcg
    11521 cccgccatcc cgcaattggc gcgggcaaac aaacgacact tttgctgaag cagggctacg
    11581 gctttgttcg tgagcatggc gacgataaag tgctggtcgt ctgggcaggg caacagtaac
    11641 ttttccggct tcccgttcgt cagtacctcg ggaagccgcc aaccaggata aaatgtcagc
    11701 cctaatcagc gttgcaggat aaagcaccgc tcactcttca acagaccgat ttgcacccca
    11761 gcaaatgtag cgttattgtt accttccttg ctacagagtt cgacagatat cccgctatga
    11821 cattctccct ttttggtgac aaatttaccc gccactccgg cattacgctg ttgatggaag
    11881 atctgaacga cggtttacgc acgcctggcg cgattatgct cggcggcggt aatccggcgc
    11941 agatcccgga aatgcaggac tacttccaga cgctactgac cgacatgctg gaaagtggca
    12001 aagcgactga tgcactgtgt aactacgacg gtccacaggg gaaaacggag ctactcacac
    12061 tgcttgccgg aatgctgcgc gagaagttgg gttgggatat cgaaccacag aatattgcac
    12121 taacaaacgg cagccagagc gcgtttttct acttatttaa cctgtttgcc ggacgccgtg
    12181 ccgatggtcg ggtcaaaaaa gtgctgttcc cgcttgcacc ggaatacatt ggctatgctg
    12241 acgccggact ggaagaagat ctgtttgtct ctgcgcgtcc gaatattgaa ctgctgccgg
    12301 aaggccagtt taaataccac gtcgattttg agcatctgca tattggcgaa gaaaccggga
    12361 tgatttgcgt ctcccggccg acgaatccaa caggcaatgt gattactgac gaagagttgc
    12421 tgaagcttga cgcgctggcg aatcaacacg gcattccgct ggtgattgat aacgcttatg
    12481 gcgtcccgtt cccgggtatc atcttcagtg aagcgcgccc gctatggaat ccgaatatcg
    12541 tgctgtgcat gagtctttcc aagctgggtc tacctggctc ccgctgcggc attatcatcg
    12601 ccaatgaaaa aatcatcacc gccatcacca atatgaacgg cattatcagc ctggcacctg
    12661 gcggtattgg tccggcgatg atgtgtgaaa tgattaagcg taacgatctg ctgcgcctgt
    12721 ctgaaacagt catcaaaccg ttttactacc agcgtgttca ggaaactatc gccatcattc
    12781 gccgctattt accggaaaat cgctgcctga ttcataaacc ggaaggagcc attttcctct
    12841 ggctatggtt taaggatttg cccattacga ccgagcagct ctatcagcgc ctgaaagcac
    12901 gcggcgtgct gatggtgccg gggcacaact tcttcccagg gctggataaa ccgtggccgc
    12961 atacgcatca atgtatgcgc atgaactacg taccagagcc ggagaaaatt gaggcggggg
    13021 tgaagattct ggcggaagag atagaaagag cctgggctga aagtcactaa aaattgcctg
    13081 atgcgctacg cttatcaggc ctacatgatt tctgcaatat attgaatttg gaagaatttg
    13141 taggccggat agggcgttta cgccgcatct ggcataaaca aagcacgcta ttccggcgtt
    13201 gagaaacgcc ggaaaacgtt tcaaacaggc tgctgccgta gccgatgccg ctgcactttc
    13261 tcgacatcca tgcactgcaa cgcctttgtc gggcaggctt caacacaggc cggtcccgtc
    13321 tcccgatgcc agcacagatc gcacttaatc gccctcgcct ttttgcgcga agaaacgacc
    13381 tccatcgcac caaacgggca agccagcata cagcttttac agccaatgca acgtgtttgt
    13441 tcaacgaaaa tatgcccttg ttcgcggctt atcgcgtcaa cagggcagac attcgcgcac
    13501 ggtgcatctt cacactgatg acaggctact gccgtggtcc agctgtggtc tttaatgaca
    13561 cgaatacggg aaataaactc gtctggtgac aacgcagcgc aatcctgatt ctcctgatgc
    13621 gacactgcgc aagccacttc acaggtacgg caaccgatac atttcgtcgc atccgcaata
    13681 ataaaccggt tcatgatcac tcctttgcac taaaacagca gagtgccaga gagcgcgtcc
    13741 ctactgcttt gatccaacga aggaaatgtt aaagttttgg cacttgtcca aagcgttatg
    13801 tgatcgcgcc caggtcatcc ctgacagtaa atcccagttc attagaaata gcctgcgcgg
    13861 tttcacgcag tggtttcagg agatttttct ctcccacctg tttcagacgt gatgtcgaaa
    13921 gcgaaatcga cacggcgtac ggcacccgcc catgaatatc aaacaccgga acagcaatac
    13981 aggagacgcc gagttcgttt tcttctctgt ccatcgccgc tccgctttca cgaatgtgcg
    14041 ccagttcgtc gaacatcgcg ggcagctcgg taatggtatt gcgggttaac ggctggatct
    14101 catgctgatg gctttcccag tatgacttca cgtagtccgg gtgaccaaac gccatgtaga
    14161 tcttgcccat tgcggaacag tagagcggca tatgctggcc aatataggca cgggttcgca
    14221 gcatcccggt tgtgggttcc agcttataaa tcaaaatagc gtgatcgtct tcgcggctgg
    14281 agaagttaat ggtttcacca gtggcgatgt tcagtgcctc aagatgcgga gcggcgatat
    14341 gaatgatatt cagcgaagac agcgccttct gcccgacggc aataaatttg gtggtcaggc
    14401 gataactccc tgcggcaggc gcggtggtca catagccgca ggactgtaat ccctgcaata
    14461 agcgatggac ggtactctta tttaaaccag ccagctccga aagatgcgcc aacggacaac
    14521 cgtttggata gttgctcaaa atctcaatca gcatcaaccc gcgaaacaga ctctggcttc
    14581 cggctggacg ctctttttcc tgcgccatct cgttctcttt ttttcccatc acttctttcc
    14641 ccattttgtc gcgtcctgat ggtagcgcaa agtgtgccgt agttcacgat ctcgacagat
    14701 aatttataac caattgattt ttatgtcttt tgaaattcat caatcagatt gctttgttaa
    14761 aaagcgatcg atatatttga aatcaagttt cgcatattga aattttaagc caaaaaagcg
    14821 atcaaaaaaa caaggaagcc tgggatgaaa gtgacatttg agcagttaaa agcagccttt
    14881 aatcgggtct taatttcacg cggcgttgac agcgaaacgg ctgacgcctg tgcagagatg
    14941 ttcgcccgca ccaccgaatc cggcgtttat tctcacggcg ttaatcgttt ccctcgtttc
    15001 attcaacaac tggaaaacgg cgatatcatt cctgatgccc aacccaaacg tataaccagc
    15061 ctcggcgcaa ttgaacagtg ggacgcccag cgttcgatcg gtaacctgac agcgaaaaag
    15121 atgatggatc gcgccattga actggctgcc gatcacggta ttggtctggt ggcactacgt
    15181 aatgccaacc actggatgcg cggcggcagc tacggctggc aggcggcgga aaaaggctat
    15241 attggcattt gctggaccaa ctccatcgcc gtaatgccgc cgtggggcgc aaaagagtgt
    15301 cgcatcggca ctaacccgct gatcgtcgcc attccttcca cgccgatcac catggtcgat
    15361 atgtcgatgt cgatgttctc ttacggcatg ttagaagtta accgtctggc aggtcgtcag
    15421 ctcccggtcg atggtggctt tgatgatgag ggcaatttga ccaaagaacc tggcgttatc
    15481 gagaagaatc gccgcatttt gccgatgggc tactggaaag gttctggcat gtcgattgtg
    15541 ctggatatga tcgctactct cctttccgac ggcgcatccg ttgccgaagt cacccaggac
    15601 aacagcgacg aatacggcat ttcacaaatt tttattgcca ttgaagtgga caagcttatc
    15661 gacggtccca cccgcgatgc caagctgcaa cgcatcatgg attacgttac tagtgccgag
    15721 cgtgctgacg aaaatcaggc cattcgctta cccggccatg aatttactac cctgctggcc
    15781 gaaaaccgcc gtaacggcat cactgttgat gacagcgtgt gggccaaaat ccaggcgtta
    15841 tgaggagata agtcatgatt tttggacata tcgcgcagcc caatccgtgc cgtttgcccg
    15901 ccgccattga aaaggtgctc gattttctgc gcgctaccga tttcaacgcc ctggagccgg
    15961 gcgttgtcga aatcgacggc aaaaatattt atacgcaaat tatcgattta accacacgcg
    16021 aagcggtggt aaaccgtccg gaagtgcatc gtcggtatat cgatattcag tttctggcgt
    16081 ggggcgaaga gaaaatcggc attgctattg atacgggaaa taataaagtc agcgaatcac
    16141 tattagagca gcgcaatatt attttttatc acgacagtga acatgaatca tttatagaaa
    16201 tgataccggg cagctacgca attttctttc cgcaggatgt tcaccgacca ggttgtatta
    16261 tgcaaacagc ctctgagata cgaaaaattg tggttaaagt cgcattaacg gcgctgaatt
    16321 aatcgaataa tcgtctacgc cagaacgcct gggtaatgtt attgctctaa ctatattaat
    16381 aattcaggtt gctgcacatg cgacttgaat tatgacagac atagcctcaa ggaatagcta
    16441 tgaaaaaaat actcga
//
LOCUS       DAPHBJ010000094        15929 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.095, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000094 DAPHBJ010000000
VERSION     DAPHBJ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.095"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(21..746)
                     /gene="phoU"
                     /locus_tag="RHZ76_002690"
     CDS             complement(21..746)
                     /gene="phoU"
                     /locus_tag="RHZ76_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDV2813305.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(761..1534)
                     /gene="pstB"
                     /locus_tag="RHZ76_002691"
     CDS             complement(761..1534)
                     /gene="pstB"
                     /locus_tag="RHZ76_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HDV2813306.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(1717..2607)
                     /gene="pstA"
                     /locus_tag="RHZ76_002692"
     CDS             complement(1717..2607)
                     /gene="pstA"
                     /locus_tag="RHZ76_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDV2813307.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(2607..3566)
                     /gene="pstC"
                     /locus_tag="RHZ76_002693"
     CDS             complement(2607..3566)
                     /gene="pstC"
                     /locus_tag="RHZ76_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HDV2813308.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(3652..4692)
                     /gene="pstS"
                     /locus_tag="RHZ76_002694"
     CDS             complement(3652..4692)
                     /gene="pstS"
                     /locus_tag="RHZ76_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDV2813309.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(5006..6835)
                     /gene="glmS"
                     /locus_tag="RHZ76_002695"
     CDS             complement(5006..6835)
                     /gene="glmS"
                     /locus_tag="RHZ76_002695"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDV2813310.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(6997..8367)
                     /gene="glmU"
                     /locus_tag="RHZ76_002696"
     CDS             complement(6997..8367)
                     /gene="glmU"
                     /locus_tag="RHZ76_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDV2813311.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(8720..9139)
                     /gene="atpC"
                     /locus_tag="RHZ76_002697"
     CDS             complement(8720..9139)
                     /gene="atpC"
                     /locus_tag="RHZ76_002697"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDV2813312.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(9160..10542)
                     /gene="atpD"
                     /locus_tag="RHZ76_002698"
     CDS             complement(9160..10542)
                     /gene="atpD"
                     /locus_tag="RHZ76_002698"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDV2813313.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(10569..11432)
                     /gene="atpG"
                     /locus_tag="RHZ76_002699"
     CDS             complement(10569..11432)
                     /gene="atpG"
                     /locus_tag="RHZ76_002699"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDV2813314.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(11483..13024)
                     /gene="atpA"
                     /locus_tag="RHZ76_002700"
     CDS             complement(11483..13024)
                     /gene="atpA"
                     /locus_tag="RHZ76_002700"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDV2813315.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(13037..13570)
                     /gene="atpH"
                     /locus_tag="RHZ76_002701"
     CDS             complement(13037..13570)
                     /gene="atpH"
                     /locus_tag="RHZ76_002701"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDV2813316.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(13585..14055)
                     /gene="atpF"
                     /locus_tag="RHZ76_002702"
     CDS             complement(13585..14055)
                     /gene="atpF"
                     /locus_tag="RHZ76_002702"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDV2813317.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(14117..14356)
                     /gene="atpE"
                     /locus_tag="RHZ76_002703"
     CDS             complement(14117..14356)
                     /gene="atpE"
                     /locus_tag="RHZ76_002703"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDV2813318.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(14403..15218)
                     /gene="atpB"
                     /locus_tag="RHZ76_002704"
     CDS             complement(14403..15218)
                     /gene="atpB"
                     /locus_tag="RHZ76_002704"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDV2813319.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(15227..15607)
                     /gene="atpI"
                     /locus_tag="RHZ76_002705"
     CDS             complement(15227..15607)
                     /gene="atpI"
                     /locus_tag="RHZ76_002705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HDV2813320.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         3830 a         4341 c         3982 g         3776 t
ORIGIN      
        1 attgggattt gtctggtgaa ttatttgtcg ctatctttcc ccgccagcag tttatccagc
       61 tcatcgccac cgacgtgacg gaaatcctgc cccttcacgt agtagaagat aaactcacaa
      121 atattctggc agcggtcgcc aatacgttcg atagaacgcg cgcagaacag cgcagtaagt
      181 acgctcggaa tggtacgcga atcttccatc atgtaggtca tcagttgacg aacaatacct
      241 tcgtattcct gatcgacttt tttatcttca cgataaatac gtaccgcttc gtcaatgtcc
      301 atccgcgcga acgcgtccag cacgtcgtgc agcatctgga tggtatgacg gcccagcgac
      361 tccagactta ccagcaacgg ctgatgctgc tgggagaatt tctccagcgc agtacggcag
      421 attttgtccg ccacgtcgcc aatacgctcc agctcggcaa tggttttact gatcaccata
      481 accagtcgca ggtcgctcgc cgtcggctga cgtttggcga taatgcgcac gcaggcttca
      541 tcgatcgcca cttccatcat gttgacgttc ttgtcgcctt cgatgacgcg cttcgccaga
      601 tcgctgtcct ggttatgcat cgcggtgatt gcatcagaaa gctgctgctc caccatgccg
      661 cccatggtca tcacctgcgt gcggatactt tccagttcgg cgttgaactg gccggaaata
      721 tgtttattaa gattgagact gtccataacg cactcctgaa tcaaccgtaa cgaccggtga
      781 tgtagtcttc tgtttgtttc ttcgctggct tggtgaacag atcgtccgtg ttgctgaact
      841 caatcaattc gcccaggtac ataaacgccg tgtggtcgga acaacgcgca gcctgctgca
      901 tgttgtgggt gacgatcacc acggtgtaat cctgcttcag ttcggtgatc agctcttcaa
      961 tacgcccggt agagataggg tcgagcgccg aacacggttc gtcgagcagc agcacttccg
     1021 ggcgaatggc gataccacgc gcaatacaca gacgctgttg ctgaccacca gagagagagt
     1081 aaccgctctg gtgcaattta tctttggttt cgttccacaa tgcggctttg gtcaatgccc
     1141 actgcacgcg ctcgtccata tcggcacggg agagcttctc aaacagacga acgccaaaag
     1201 cgatgttgtc gtagatggac atcgggaacg gcgtcggttt ctggaacacc atgcccactt
     1261 tcgcacgcag cagggcgata tcctgagagt tggtcaggat gttgtcgcca tcaagcagaa
     1321 tttcaccttc cgcacgctgc tccgggtaca gttcaaacat tttgttgaag gtacgcagca
     1381 gcgtcgattt accgcagccg gacggcccga taaacgccgt aacctggttt ttggcgatat
     1441 ccaggttgat gtttttcagg gcatggaatt tgccgtagta gaagttcaaa ttacgaacct
     1501 gaattttact cggggcagtt tcaaccatac tcattgcaat cttttcctca tcgtgcctga
     1561 tgcacttcgc taatcaggct taccgtaggc cggatcaggc gttcacgccg catccggcaa
     1621 agtgttaacc gtgtacagag tcaaaaaggc actcggtcgg tcccctcgcc cctctgggga
     1681 gagggttagg gtgaggggaa aaccgtgtca gcaatatcaa ccgtgtttat tcttcgcaaa
     1741 aacaacgcgc gccagaatgt tcagcagcag tacgcacagg gtaatgatca ataccccggc
     1801 ccaggccaat tgctgccatt ccgcaaacgg gctcatcgca aacttaaaga tcgtcaccgg
     1861 caggttggcg atcggctgca tcatgtccgt gctccagaac tggttggaga gcgcggtaaa
     1921 cagcagcggc gcggtttcac cagcaatacg ggcaatcgcc agcaggatac cggtcataat
     1981 cccggacacc gacgctttca gcgtaatcgc agagatcatc ttccacttcg gtgtacccag
     2041 cgcataagcc gcttcacgca ggctgtccgg caccagtttc agcatgttct cggtggtgcg
     2101 gataacaatc ggcacctgca acaacgccag ggcaatcacg cccgcccagc cggagaagtg
     2161 ctccatctgc gccaccacaa tggtgtaaac aaacagacca accacaatcg acggcgcaga
     2221 gagcagaatg tcgttaatga agcgaatcac ttctgccagc caggatttac gaccatattc
     2281 cgccagataa atccccgcca taatgcccag cggcgtaccg aataccgtgg cccacaaaat
     2341 taacaggccg ctacccgcca gggcgttcgc cagaccacca ccttccgtat tgggcggcgg
     2401 cgtcatttca gtgaacagcg ccagcgacat accatcgata ccgcgagtga tggtggacat
     2461 taaaatccag atcagccaga acagaccgaa ggccatcgtc gccatcgaga gcgtcagcgc
     2521 aatacggttt ttgaggcggc gacgcgcctg cattttgcgg cgagattcag ccagcgccgc
     2581 agtggtttgc atttcaacca tagccattag cgtgccccct cattcttagc caggcgcata
     2641 atcataaact tcgatgcggc gaggacgatg aaggtaatca caaacaggat caggcccagt
     2701 tccatcagtg cggcaacgtg cagaccggat tccgcttccg caaattcgtt cgccagcgca
     2761 gaggtgatac tgttgcccgg catatacagc gaggcgctgt cgagctggta ggtgttaccg
     2821 atgataaagg tcaccgccat ggtttcaccg agcgcgcggc ccagccccag catgatgccg
     2881 ccgataacac catttttggt gaacggaaga acgatacgcc agataacttc ccaggtggtg
     2941 cagccaatac cgtaggccga ctctttcatc atcaccgggg tttgttcgaa cacatcacgc
     3001 attaccgccg caatgtacgg aataatcatg atggcgagga tcacgcctgc cgcgaggata
     3061 ccgataccaa atgcggggcc agagaacagc gcgccaacaa tcgggatatt cgacatgata
     3121 ttgccgaccg gctcctgaaa gtaaacggcg aacagcggcg caaagataaa caggccccac
     3181 atgccgtaaa cgatacttgg aatggctgcc agcagctcaa tggcgatacc cagcgggcgt
     3241 ttcagccagc cgggcgcaag ctcagtcagg aacagggcga taccgaaact caccgggacg
     3301 gcgatcagca gcgcgataaa cgaagtcacc agcgtaccgt agatcggcac caacgccccg
     3361 tagatatcgt tcggtgcatc ccactctttg gtccacagga aagccagacc aaatttctga
     3421 atgctcggcc aggaggagat gatcagagag acaataatgc cacccaacat caatagcaca
     3481 atcagcgccg ccagttttac cagcacgctg aaaattatgt cgcccttttt acccggtggg
     3541 ttaaaagcag gcttggttgc agccataagt tactcttcag ttaaacgcgt ttacaaagtc
     3601 attttgtaat gccggataag ccgttaatgc cttatccggc ctggagtttt attagtacag
     3661 cggcttaccg ctactgtctt taatattggt cttccacgca gcgcgaacct gttcaacgac
     3721 gctatccggc aggctggcat aatccaggtc gttcgcctgt ttagccccgg ttttgtacgc
     3781 ccagtcgaag aacttcagca cttctgtgcc ttgttctggt ttcttctgat ctttgtggat
     3841 cagaatgaac gtggtagagg taataggcca tgcatcttcg cctttctggt tggtcagatc
     3901 ctgagcgaag gttttgctcc agtctgcacc ttttgctgca ttagcgaagt tttcttcggt
     3961 cggactaacc ggtttaccat cagcggagat cagtttggtg tacgccaggt tgttctgctt
     4021 agcgtaagca tattcaacgt aaccaattgc acccggcaga cgctgaacga acgcggcgat
     4081 accgtcatta cctttaccgc ccagaccgat cggccatttt acggtagagc cagtaccaac
     4141 gttgtttttc cactcttcgt tcactttcgc caggtagctg gtgaagacga aggaagtccc
     4201 ggagccatct gcgcggcgta ctacagcaat gttctgtgaa ggcagtttca gacccggatt
     4261 cagtttggcg atggcttcat catcccactt cttgattttg cccaggtaga tgtcgccgag
     4321 ggttttacca tccagcacca gttcgccaga cttcagccct ggaatgttaa cagccagcac
     4381 cacgccgcca atcacggtcg ggaactggaa caggccttcc tgagccaatt tttcatcaga
     4441 cagcggcgcg tcagaggcac caaaatcaac ggtattagcg ataatctgtt ttacgccacc
     4501 ggaagaacct ataccctggt agttaacttt attaccggtt tctttctggt aagtgtcagc
     4561 ccatttggca tacaccggcg cagggaaggt tgcacctgca cctgtcaggc ttgcttctgc
     4621 aaacacagag aaagcgctca tcgataaggt cgcggcgaca acagttgcga cggtggtacg
     4681 cataactttc ataatgtctc ctgggaggat tcataaagca ttgtttgttg gctacgagaa
     4741 gcaaaatagg acaaacaggt gacagttata tgtaaggaat atgacagttt tatgacagag
     4801 agataaagtc ttcagtctga tttaaataag cgttgatatt cagtcaatta caaacattaa
     4861 taacgaagag atgacagaaa aattttcatt ctgtgacaga gaaaaagtag ccgaagatga
     4921 cggtttgtca catggagttg gcaggatgtt tgattaaaaa cataacagga agaaaaatgc
     4981 cccgcttacg cagggcatcc atttattact caaccgtaac cgattttgcc aggttacgcg
     5041 gctggtcaac gtcggtacct ttgatcagcg cgacgtgata agccagtagc tgcagcggaa
     5101 cggtgtagaa gattggtgca atcacctctt ccacatgcgg catctcgatg atgtgcatgt
     5161 tatcgctact tacaaaaccc gcatcctgat cggcgaagac atacaactga ccgccacgcg
     5221 cgcgaacttc ttcaatgttg gattttagtt tttccagcaa ttcgttgttc ggtgcaacga
     5281 cgataaccgg catatcggca tcaatcagcg ccagcggacc gtgtttcagt tcacctgcag
     5341 cgtaggcttc agcgtgaatg taagagatct ctttcagctt caatgcgcct tccagcgcga
     5401 ttgggtactg atcgccacgg cccaggaaca gcgcgtgatg tttgtcagag aaatcttctg
     5461 ccagagcttc aatgcgtttg tcctgagaca gcatctgctc aatacggctc ggcaacgcct
     5521 gcagaccatg cacaatgtca tgttcaatgg aggcatccag acctttcagg cgagacagct
     5581 tcgccaccag catcaacagc acagttaact gagtggtgaa tgctttagtg gatgccacgc
     5641 cgatttctgt acccgcgttg gtcattagcg ccagatcgga ttcgcgcacc agagaagaac
     5701 ccggaacgtt acagattgcc agtgaaccaa ggtaacccag ctctttcgac agacgcaggc
     5761 cagccagggt atccgcggtt tcgccagact gtgacaaggt gatcatcagg ctgttacgac
     5821 gcacggcaga tttgcgatag cggaattcag aggcgatttc gacgtcgcac ggaatacctg
     5881 ctagcgattc aaaccagtag cgggaaacca taccggagtt ataagaagta ccacaggcga
     5941 ggatctgaat atgctcaacc ttcgacagca gttcgtcggc gttcggtccc agctcgctta
     6001 aatcaacctg accgtggctg atgcgtccgg taagggtgtt tttgatcgcg ttcggctgtt
     6061 cgtagatctc tttctgcatg tagtgacggt aaatgccttt atcgcccgcg tcatattgca
     6121 gattggattc gatatcctga cgttttactt ccgcgccagt tttatcgaag atgtttaccg
     6181 aacggcgagt gatttccgca atatcgccct cttcaaggaa gataaagcga cgggtcaccg
     6241 gcaacagcgc cagctggtca gaagcgataa agttttcgcc catccccagg ccaatcacca
     6301 gcggactacc agaacgtgcc gccagcaggg tatccgggtg acgggagtcc atgatcactg
     6361 taccgtacgc accacgcagc tgcgggatag cacgcagaac ggcctcacgc agagtcccgc
     6421 cttgtttcag ctcccagttc accagatggg caatcacttc ggtgtcggtt tcagaaacga
     6481 aggtatagcc acgcgctttt agctcttcac gcagcggttc atggttttcg atgatgccgt
     6541 tatgcaccac cacaatgtgt tcagaaacat gcggatgcgc attcacttct gaaggttcac
     6601 cgtgggtcgc ccagcgagtg tgagcaatac cagtgccgcc atgcagagga tgttcttccg
     6661 ctgcctgtgc cagcatctgg actttaccga ggcgacgcag gcgggtcata tgaccttctg
     6721 catcaacaac ggccagaccg gcagagtcat atccgcggta ttccagacga cgtaaacctt
     6781 caagaaggat ttctgctaca tcacgttgcg cgatcgcgcc aacaattcca cacatagttt
     6841 ttgattccga tttatatcgt tgtcggtcaa cctgtatgcc cgttttttcg ggcgccccga
     6901 gccttgtaga gagtggggtt atttttatag ttactgcttg tgggagggag attatgttat
     6961 ctcctcatcc catgtgaccg ggttagccgg ccagaatcac tttttcttta ccggacgacg
     7021 ccagccttct ttctgagtct gcggcacacg gctgatagct aatgcatttt cgccgacatt
     7081 acgcgtcaca gttgtacccg cagcaatggt cgcgcctttg cctactgtta ccggggccac
     7141 cagctgagtg tcggaaccaa caaacacatc gtcgccgata atggtcttaa atttattcgc
     7201 accatcgtag ttgcaggtaa tggttcccgc gccgatgtta acgttatcgc caatttccgc
     7261 atcgcccagg taagtcagat gaccagcttt cgagccttta cccagacgcg cttttttcat
     7321 ctcaacgaag ttaccgacgt gagcaccttc cagcaactca gcaccaggac gcaaacgggc
     7381 aaacgggcca atggtacagg ccgctgccag attcgcatct tccacaacgg tatacggact
     7441 gatttcgcaa tcatcgccaa tcacgctgtt tttaatcacg caaccggtgc caattttcac
     7501 gcgatgaccg agagtcacgt tgccctcgat gataacgtta gtatcaattt caacatcgcg
     7561 cccgtgagtt agcgtaccac gcagatcaaa acgcgctgga tcgcgcagca taacgcctgc
     7621 taacagcagt ttttcagcct gttcggactg ataaacacgc tccagacggg agagttgcag
     7681 gcggttattc acgccttcta cttcgcttaa acgttgcgga tgaacggcga cgatttcacg
     7741 cccttcctga tacgccagcg caataatgtc ggtgatgtag tattcgccct gagcattatt
     7801 gttggtcagc ttcgccagcc agcgtttcat atctgcgccg ttggcaatca gaatgccggt
     7861 gttgatctcc tgaatctgac gctgctcgtc ggtggcatct ttgtgctcaa caatgccggt
     7921 aactttgccg ttttcacggg tgatacgtcc ataaccggtc ggatcatcca gtttcaccgt
     7981 cagcagacca atgccaccct gcggtttagc atcacgcaga cgctggagtg tttcgacaga
     8041 gatcagcggc acgtcgccgt agagcattaa aatgtcttca tcatcggcaa agaaaggtgc
     8101 ggcctgctgc attgcatgac ccgtacccag ctgctctgcc tgaagcaccc agttaaggtt
     8161 gtcgtctttc agcgcctgtt ttagcagatc gccgccgtga ccgtacacca ggtgaacgtg
     8221 cgctgcgcct aattcattcg cagcatcaat gacatgctga accatcgctt tcccggcaag
     8281 ggtatgcagc actttcggaa gatcggaata catgcgcgtg cctttgcctg cggcaaggat
     8341 cactacgctc atagcattat tcaacatacg cgtcctgact gtaatttgag aacgaattta
     8401 aaccgcttca ccttgaaaaa actacatttt tttcatcgtg aaatggacag aggataaatt
     8461 gttcaataac ggattatccc cgctgacgaa agcgccattt tcgaccatcg tcacgccttt
     8521 tgtctgcaga aaattaaggc agtaccttaa tttaagcgtc aggtggatgt ttttgctctt
     8581 atttcgatca atgaataaac aggaaaacag gggggtttta tatcagcagg atctatgtga
     8641 acgctattca ggacgggtca cacgcgcaaa aaaaagccag cctgtttcca gactggcttt
     8701 tgtgcttttc aagccggtgt tacatcgctt ttttggtcaa ctcgataacg cgcagctgcg
     8761 cgatcgcttt ggccagttcc gcagacgcct gagcgtaatc tacgtcgccg tgagagctgc
     8821 taatgtgctc ttcagcctta cgtttcgctt ccatggctcg cgcttcgtcg agatcctggc
     8881 cgcgaattgc ggtgtcggcc agaacggtca cgttgccagg ctgcacttca agaatgccgc
     8941 cagacagata gataaactct tcgtgaccgt gctgtttcac gatgcgaatc ataccaggct
     9001 taatggcggt gagcagcggt gcgtggccag ggtagatccc cagttcacct tcgctacccg
     9061 ttacctggat tttctcgacc agaccagaga acatttgttg ctctgcgctg acgacgtcca
     9121 ggtggtaagt cattgccata tcaccctccg attaaggcgt taaagttttt tggctttttc
     9181 cacagcttct tcgatggaac cgaccatgta gaacgcctgc tccggcaggt gatcgtattc
     9241 gccttccatg atgcctttaa agccacggat ggtgtctttc agggagacgt atttacccgg
     9301 agaaccggtg aatacttctg ccacgaagaa cggctgggac aggaagcgct ggatcttacg
     9361 agcacgcgct accaccagtt tgtcttcttc agacagttca tccataccca ggatggcgat
     9421 gatgtctttc agttcctgat aacgttgcag gatggactga acgccacgcg cggtgtcgta
     9481 gtgttcctga ccaaccacca gcgggtccag ctgacggctg gtggagtcca gcgggtcaac
     9541 ggccgggtag atacccagag acgcgatctg acggctcagt accacggttg cgtcaaggtg
     9601 cgcaaaggtg gttgccggag acgggtcagt caagtcatcc gcaggtacgt atactgcctg
     9661 tacggaggtg atagaaccag ttttggtgga ggtgatacgt tcctgcagaa cgcccatctc
     9721 ttccgccagg gtcggctgat aacctaccgc tgaaggcata cggcccagca gtgcggatac
     9781 ttccgtaccg gccagggtgt aacgatagat gttgtcaacg aacagcagaa cgtcacgacc
     9841 ttcgtcacgg aatttctcag ccatggtcag accggtcaga gcaacgcgca gacggtttcc
     9901 cggcggctcg ttcatctggc catacaccag ggatactttg tcgataacgt tggagtcggt
     9961 catttcgtgg tagaagtcgt taccctcacg agtacgttca cctacgcccg caaacacaga
    10021 gtaaccggag tgctcgatcg cgatgttacg aatgagctcc atcatgttta cggttttacc
    10081 tacacccgca ccaccgaaca gaccaacttt accgccctta gcgaacggac acatcaggtc
    10141 gataactttg ataccggttt ccagcagttc ctgagagttt gacagctctt cgtaggaagg
    10201 tgctgcgcgg tgaatcgccc aacgctcttc ttcaccgatc tcgcctttca tgtcgaccgg
    10261 ttcacccagt acgttcatga tacggcccag agtcgcttta cctaccggga cttcaatcgg
    10321 gtgttcgagg tcttttacat ccagaccgcg acgcagaccg tcggaggaac ccattgcgat
    10381 ggtacgtacg ataccgccgc cgagctgctg ctgaacttcc agcaccagac gctcattacc
    10441 attttgcacc tcaagagcat cgtacacgcg cggtacggca tcctgaggga attcgacgtc
    10501 aactacggcg ccgattacct ggacaatctt tccagtagcc atcttaaatc ctctacgaaa
    10561 taacctgttt aaaccgcggc ggcccccgag acgatctcgg tgagttcctg agtaatgctg
    10621 gcctgacgag ctttgttgta taccaactgc agctctttaa tcaggctgcc gccattgtcg
    10681 gtcgcggctt tcatcgccac catacgggcg gcctgctcgc tggccaggtt ttcaaccacg
    10741 ccctgataaa cctgagattc gacataacga cgcagcaggg tatccagcaa cgccttcgga
    10801 tcgggttcgt acaggtaatc ccaggattta tgtttcagat catcatcatc tgatgccggt
    10861 aacggcagca gctggctgat ggtcggaacc tgagacatgg tgttaataaa tttgttgctg
    10921 acaatgtaaa gcttgtccag acggccttcg tcgtaggcct gcaacatcac ttttaccgga
    10981 ccgatcagtt cggacaggga agggttatcc cccatgccgg tgacctgggc aacaacattg
    11041 ccgcccacgg agttgaagaa cgacacgcct ttcgagccga tcattgcgag gtcgcattga
    11101 acgcctttgt cggtccaggt cttcatttcc gctagcagtt ttttgaacag gttaatgttc
    11161 aaaccaccgc acaaaccacg gtcggtcgac accaccaggt agcccacgcg tttaacgtcg
    11221 cggtcttcca ggtaagggtg cttatattcc agattaccgt gtgcaaggtg accaatcact
    11281 ttgcgcatgg tttctgcata aggacggctg gccgccatgc gatcctgcga tttacgcatt
    11341 ttggaagcgg cgaccatctc catcgcttta gtgatctttt gcgtgttctg gacgcttgcg
    11401 atcttactac gtatctcttt tgcgccggcc atgagcttct cctcaatgcc ttgcggcctg
    11461 ccctaaggca agccgccaga cgttaccagg attgggttgc tttgaaggaa tcgaggatgc
    11521 ctttcagctt gccttcgatt tcgtcgttgt agccaccggt ctggttgatc tcttgcatca
    11581 acggagcgtg atcacggtcg acgtaagcca gcagagcggc ttcgaagctg ccaattttcg
    11641 acagttcaac atccgccagg taaccacgtt ctgctgcgaa cagaaccaga gactgctgcg
    11701 caacggacat cggcgcatac tgtttctgtt tcagcagttc ggtcactttc tgaccgtggt
    11761 caagctgctt acgtgttgca tcgtcaaggt cggatgcaaa ctgagagaac gctgccagtt
    11821 cacgatactg tgccagagcg gtacggatac caccggacag ttttttcatg atcttggtct
    11881 gtgctgcacc accaacacgg gatacggaaa tacccgggtt aaccgcagga cgaataccgg
    11941 cgttgaacag gttggtttcc aggaagatct gaccatcggt aatggagatt acgttggtcg
    12001 gaacgaacgc agaaacgtca cccgcctgag tttcgataat cggcagtgcg gtcagagaac
    12061 cggttttccc tttcacttca cctttggtga aggcttcaac gtattcggcg ttaacacgtg
    12121 cagcacgctc cagcagacga gagtggaggt agaaaacgtc gcccgggaat gcttcacgtc
    12181 ctggcggacg acggagcagc agggagatct gacggtaagc aacagcctgt ttagacaggt
    12241 catcgtaaat gatcagcgca tcttcaccgc ggtcacggaa gtattcgccc attgcgcaac
    12301 cggcatacgg tgccaggtat tgcagtgcag cggattcaga cgcggttgct accacaacga
    12361 tggtgttagc cagtgcgccg tgctcttcca gtttacgtac cacgttagaa atggtggacg
    12421 ctttctggcc gatagcgaca tagatacatt tgataccgga atcgcgctgg ttgatgatgg
    12481 catcgatagc cagtgcggtt ttacctgtct gacggtcacc gatgatcaat tcacgctgac
    12541 cacgaccgat tgggatcatg gagtcaacgg ctttataacc ggtctgtacc ggctgatcta
    12601 cggactgacg ttcgataacg cccggagcga ttgcttctac agcagagaag ccgtcgtgat
    12661 ccagcggacc tttaccgtcg attggtgcac ccagagtgtt aaccacacgg cccagcaggc
    12721 cacggccaac cggaacttcc aggatacggc cagtacactt aactttcatg ccttcggcaa
    12781 ggtcagcgta cggacccata acaaccgcac ctacagagtc gcgctcgagg ttcagtgcga
    12841 tagcgtaacg gttacccggc agggagatca tttcaccctg catacaatcg gccaggccgt
    12901 gaatgcggat aacaccgtca cttacagaaa caatagtacc ttcgttgtga gcttcactca
    12961 caacattgaa ctgagcaatg cgctgcttga tcagttcgct gatttcggtg gaattcagtt
    13021 gcatgctcca gtccccttaa gactgcaaga cgtctgcaag gcgctcaaga cgaccgcgta
    13081 cgctgccatc aatgaccata tcacccgctc ggatgataac gcctgccatt acagacttat
    13141 cgattttgca attcagctta actttgcgtg acagacgttt ttccatcgca gcagaaattt
    13201 tcgcgagctg ttgttcactc agtgcggcag cggaaatgac gtctacctca gcggtagcct
    13261 cactcacggc acgcaggtga ataaactgct ccagaacatc cgggagcgcg ttaagacgac
    13321 cattttcagc cataacccga atcaggttct gaccgttttc gtccagttgc tcaccacaaa
    13381 ctgcgataaa cgactcggcg agcgtttctg gcgcaagcgc gccagagaga agctctgcca
    13441 tttgttcgtt tttggttacc tcggcggcaa acgccagcat gtcctgccag cgttctacac
    13501 tttggtgttc gacggcaaag tcaaaagctg ctttggcgta ggggcgagct accgtaataa
    13561 attcagacat cagcccctcc ctccttacag ttcagcgaca agtttatcca cgatgtcgct
    13621 gttagcagct tcatccacgg aacgttcgat gatcttctcg gcgccagcaa cagccaggat
    13681 agcaacttgc ttacgcagct cttcacgggc acgtttacgc tcggcttcaa tttccgcctg
    13741 cgcctgggcc acgattttag tacgttcctg ttctgcctca gctttcgctt cgtccagaat
    13801 ctgcgagcgg cgtttgttcg cctgctcgat gattacctgg gcttccgctt tcgctttttt
    13861 cagctggtcg gtcgcgctgg cctttgcaag gtcaaggtcc ttatgtgctc gttctgcgga
    13921 agcaaggccg tcagcaattt ctttttgacg tttttcgatg gctgccatta atggcggcca
    13981 tacgtacttc atgcagaaca gaacgaacag gacaaacgcg atggcctggc cgaggattgt
    14041 tgcgttaaga ttcacagcac aatgcctcta tttagttaac gttctgatat tgctctttaa
    14101 ataaaagcaa cgcttactac gcgacagcga acatcacgta cagacccaga cctacagcga
    14161 tcatcgggat agcatccacc agacccataa cgataaagaa ctgagtacgc agcagaggaa
    14221 tcagatcagg ttgacgcgct gcgccttcca ggaatttacc cccgaggatg ccgataccga
    14281 tcgcagcacc gattgccgcc agacccatca tcacagcggc agccatgtac agcagatcca
    14341 tattcaggtt ttccatgaca gtctccagtt tgtttcagtt aaaacgtagt agtgttggta
    14401 aattaatgtt cttcagacgc catcgacaga tagacgatcg tcagaaccat gaagatgaag
    14461 gcttgcagcg taatgatcag gatgtggaaa atggcccacg gcacattcag gatccactgt
    14521 gaccaccacg gcaacagacc agcaatcaga atgaaaatca gctcaccggc atacatgtta
    14581 ccgaacagtc gcaaaccgag tgaaactggt ttggacagca ggcttacccc ttcaaggatt
    14641 aagttgacag gaatgaacgc ccagtgattg aacggctgca gcgtcaactc tttcgtgaag
    14701 ccgccgatgc ctttcatttt gatgctgtag aacagaatca ggataaatac gcccagtgcc
    14761 atagacagcg ttacgttcac gtccgcagac ggaaccacac gcagtgcagg cagacccagt
    14821 acatgttcag caatgtacgg cagcaggtcg ataggcagta aatccatcag gttcatcagg
    14881 aatacccaga cgaagatcgt cagggccagc ggagcaatca gcttgctttt gccatggtac
    14941 atgtctttca cgctaccatt aacaaagccg atcaccagct caatcgcggt ctgaaactta
    15001 cctggcacac cgctggtcgc ctttttggct acgctacgga ataaaaccag gaacaacaga
    15061 cccagcacca ccgagaagaa catggagtca atattgattg tccagaaggt ggctgggggg
    15121 ttttgtggat ccaccagcga gaatgtacgc aggtccagct gaaggttatt caggtggtgt
    15181 cctatgtaat cctgcggcgt catattttct gaagccatga tgccttttac cctttgttgt
    15241 taattacagc cggtgccagt atctgaacca ccagcaccaa aacccacgta acgatcagcg
    15301 gcaagaatac cgcctttaaa accgccaacg ccaccaccag taacaccaac atcgccagaa
    15361 ctttgaaagc ttcgccaaat gcgaatgtcc aggccacccg gcctttcgct ggtgtatgcg
    15421 cctggtgacg ccaggcaaat atcataaaca aaacgttagg cagaaagact gccaggcccc
    15481 cgcttattgc agagacgccc cagaaggggt ctttgaggct gaacagcaat ccacttgcta
    15541 tcaccaccag taactgaacg agcagaagct tccgagcaac gtttcgactc acgagcgaca
    15601 cagacatcac gtttttcact cctgctccct tcgaggtatg ccgcgtgtcg tataaaactt
    15661 tctttaaggc ttagagtcaa gcatcaaaaa gcggtcaaat tatacggtgc gcccccgtga
    15721 tttcaaacaa taagtagcca aaaggtgaat aaatgtttaa atatttttcc agtgcataca
    15781 attgcgactt ttctgctaac cctgttcgat catgaaaaac tgtaaataac gcgtaaacac
    15841 tggtgataaa gcgtgcttca gatcacatat tgcgcatgtt cgcgcacagc atatttattt
    15901 acttggcaaa tgatgccttt gcaagttta
//
LOCUS       DAPHBJ010000095        15914 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.096, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000095 DAPHBJ010000000
VERSION     DAPHBJ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.096"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            135..569
                     /gene="slyA"
                     /locus_tag="RHZ76_002706"
     CDS             135..569
                     /gene="slyA"
                     /locus_tag="RHZ76_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HDV2813321.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(616..1083)
                     /gene="slyB"
                     /locus_tag="RHZ76_002707"
     CDS             complement(616..1083)
                     /gene="slyB"
                     /locus_tag="RHZ76_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HDV2813322.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            1357..2466
                     /gene="anmK"
                     /locus_tag="RHZ76_002708"
     CDS             1357..2466
                     /gene="anmK"
                     /locus_tag="RHZ76_002708"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDV2813323.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            2564..2893
                     /gene="mliC"
                     /locus_tag="RHZ76_002709"
     CDS             2564..2893
                     /gene="mliC"
                     /locus_tag="RHZ76_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HDV2813324.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            2952..3608
                     /gene="pdxH"
                     /locus_tag="RHZ76_002710"
     CDS             2952..3608
                     /gene="pdxH"
                     /locus_tag="RHZ76_002710"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HDV2813325.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            3737..5011
                     /gene="tyrS"
                     /locus_tag="RHZ76_002711"
     CDS             3737..5011
                     /gene="tyrS"
                     /locus_tag="RHZ76_002711"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDV2813326.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            5070..5933
                     /gene="pdxY"
                     /locus_tag="RHZ76_002712"
     CDS             5070..5933
                     /gene="pdxY"
                     /locus_tag="RHZ76_002712"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HDV2813327.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(5977..6582)
                     /gene="gstA"
                     /locus_tag="RHZ76_002713"
     CDS             complement(5977..6582)
                     /gene="gstA"
                     /locus_tag="RHZ76_002713"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HDV2813328.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(6688..8190)
                     /gene="dtpA"
                     /locus_tag="RHZ76_002714"
     CDS             complement(6688..8190)
                     /gene="dtpA"
                     /locus_tag="RHZ76_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HDV2813329.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(8801..9436)
                     /gene="nth"
                     /locus_tag="RHZ76_002715"
     CDS             complement(8801..9436)
                     /gene="nth"
                     /locus_tag="RHZ76_002715"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDV2813330.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(9436..10131)
                     /gene="rsxE"
                     /locus_tag="RHZ76_002716"
     CDS             complement(9436..10131)
                     /gene="rsxE"
                     /locus_tag="RHZ76_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HDV2813331.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(10135..10755)
                     /gene="rsxG"
                     /locus_tag="RHZ76_002717"
     CDS             complement(10135..10755)
                     /gene="rsxG"
                     /locus_tag="RHZ76_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HDV2813332.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQNDKPVAAVLETTAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPSQLSQLPACGE"
     gene            complement(10759..11817)
                     /gene="rsxD"
                     /locus_tag="RHZ76_002718"
     CDS             complement(10759..11817)
                     /gene="rsxD"
                     /locus_tag="RHZ76_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HDV2813333.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(11818..13944)
                     /gene="rsxC"
                     /locus_tag="RHZ76_002719"
     CDS             complement(11818..13944)
                     /gene="rsxC"
                     /locus_tag="RHZ76_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDV2813334.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPATKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAIARAKARKREQQPANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAVPEEQVDP
                     RKAAVAAAIARAQAKKAAQQKVVYED"
     gene            complement(13937..14515)
                     /gene="rsxB"
                     /locus_tag="RHZ76_002720"
     CDS             complement(13937..14515)
                     /gene="rsxB"
                     /locus_tag="RHZ76_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HDV2813335.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     ITPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(14515..15096)
                     /gene="rsxA"
                     /locus_tag="RHZ76_002721"
     CDS             complement(14515..15096)
                     /gene="rsxA"
                     /locus_tag="RHZ76_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDV2813336.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(15173..15613)
                     /locus_tag="RHZ76_002722"
     CDS             complement(15173..15613)
                     /locus_tag="RHZ76_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HDV2813337.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(15699..15914)
                     /gene="ydgT"
                     /locus_tag="RHZ76_002723"
     CDS             complement(15699..15914)
                     /gene="ydgT"
                     /locus_tag="RHZ76_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HDV2813338.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
BASE COUNT         4048 a         4054 c         4048 g         3764 t
ORIGIN      
        1 tttacgcgca atttctgaaa aatatgtctg acggtaacca aatgcagcaa tacatttgtt
       61 ttagcaatac aattgctgca cactattcta aaagccgcat aatatcttag caagctaatt
      121 ataaggagat gaaattggaa tcgccactag gttctgatct ggcacggttg gtgcgcatat
      181 ggcgtgctct gatagaccat cgcctgaaac cgctggagtt aacacaaacc cattgggtta
      241 cgttacacaa tatccatcag ttacctccag accagtcgca aattcaactg gcaaaagcga
      301 ttggcatcga gcagccatca ctggtccgta ctctggacca actggaagaa aaagggttaa
      361 tttcgcgtca aacttgtgcc agcgatcgtc gggctaaacg tattaaactg acggaaaagg
      421 cagagccgct gatcagcgaa atggaagctg ttattaacaa aacccgcgcg gaaatattac
      481 atggcatctc cgcagaggaa ctggagcaac tgattacgct catcgcaaaa cttgagcata
      541 atatcattga gttacaggcc aaagggtgaa atgaaggggg cgtgtggtca gtaacctgac
      601 cacacgcaaa cttatttagc gcggagaaac ggtcacctga ctgccattgc tggccagtac
      661 gacacgttgg cccggagaga aacgagtgtt gccttgtttc tgtaccacca tgatggtatt
      721 accatcgtct ttacgaattt ccagctcgac accctgcgtt ttgttcattg cactctgtac
      781 gccctgacca gctacgccac ctgcaacagc gcctgctgca gtagccagag aacgcccggt
      841 tccgccacca acagtattcc ccaggaaacc accaagaaca gcaccgccaa ttgcaccgat
      901 aacgttggaa tcatcaccgc cctgaatctg taccggacgt acgttaacga tggtgccata
      961 gctgacattc tgtacttgtt tcgcttcaga agcggtataa acatcccctg acagggtgtc
     1021 gttattaaca caaccgacaa gagacagacc taccattgaa acaaccaata cgcgtttaat
     1081 cattgaaaaa tctcctgttc accatgaaac gccacgcaag catccctcat ggtcaaagta
     1141 tatggcatat gtgaatccat ggttacatgt tctaccaaat atgagcaaat cataatgaaa
     1201 gtaggatcaa ccaattataa acaaaacatt catgatgatt attgatttga agataatgtt
     1261 ttactaccaa cggtaaaaat cggtcattcc gcatggtatt cacgaaaagt ttatgtttga
     1321 gtggtgcgca aacgtcagat tgaaaaagga tgaactatga aatcgggccg ctttattggc
     1381 gttatgtcag gcaccagcct tgatggtgtt gatgttgtgt tggcgacaat tgatgaacac
     1441 cgggtcgcac agctggcaag tttgagttgg ccgatcccgg tatctctgaa acaggctgta
     1501 ctggatattt gccagggcca gcagcttaca ctttcgcagt ttggacagct tgatactcaa
     1561 ctcgggcgac tttttgctga tgcggtcaat gccttgctta aggaacaaaa tctgcaggca
     1621 agagatatag ttgcgatcgg ttgtcacggt caaaccgtct ggcatgaacc gacgggcgtg
     1681 gcaccacaca ctttacagat tggcgataac aatcaaattg tggcacgcac cggaattacg
     1741 gttgtcggtg attttcgccg tcgcgatatt gccttgggag gacaaggcgc accgctggta
     1801 cctgcgttcc atcatgcgct gctggctcac ccaaccgagc gacgaatggt gctcaatatt
     1861 ggcggcatcg ccaatctgtc actgctcatt cctgggcagc cggttggggg ctacgatacc
     1921 ggtcctggta acatgctgat ggatgcctgg atctggcgtc aggccggtaa accttacgat
     1981 aaagatgccg agtgggcacg ggcgggtaaa gttattctcc cactgctgca aaatatgctc
     2041 agcgacccgt atttctcgca acctgcaccg aaaagcaccg gacgcgaata ctttaactat
     2101 ggttggctgg agcgccattt gcgccatttt ccgggtgttg atccccgaga tgtgcaggcg
     2161 acactggcag aactcaccgc cgtgaccatt tctgaacaag ttttgttgag cggtggctgc
     2221 gaacgattga tggtatgtgg tggaggtagt cgtaatccgc tactcatggc gcgtctggcg
     2281 gcattactgc caggcacaga agtcaccacc accgatgccg ttggcattag tggcgatgac
     2341 atggaagcat tggctttcgc ctggcttgcc tggcggacgc tggcgggatt accaggaaat
     2401 ctgccttccg tcactggcgc aagccaggag acggtactgg gggctatttt ccccgctaac
     2461 ccgtgatatc agagttaact gaatttttct taccgcgtct gccgttacac ttggaacgtt
     2521 agggagggcg tattgccctc cagaccagga aagtcttcgg gatatgacta tgaaaaaact
     2581 gttaattatc attttgccgg tccttctctc cggctgtagc gcctttaatc agctggttga
     2641 gcgcatgcaa accgatacgc tggaatacca gtgtgatgaa aaaccgttga cggtcaaact
     2701 gaataatccg cgccaggagg tcagttttgt ttacgataat caactactgc atctcaaaca
     2761 gggcatttca gcctctggcg cgcgttacac tgacggaatc tatgttttct ggtcgaaagg
     2821 cgatgaagcg actgtctata aacgcgaccg catcgtcttg aataactgtc agttacaaaa
     2881 tccacagcgt tgagattttt ccaggggcgg cgcacaatag cgtcacccac tgacaatccg
     2941 taaagaaaac catgtctgat aacgacgaat tgcagcaaat cgcgcatctg cgccgtgaat
     3001 acaccaaagg cgggttacgc cgccgcgatc ttcccgccga tccattaacc ctttttgaac
     3061 gttggctctc tcaggcttgt gaagccaaac tggcggaccc taccgcgatg gtggtcgcta
     3121 ccgtggatga acatggtcag ccttatcagc gcatcgtttt actcaaacat tacgacgaaa
     3181 aaggcatggt gttttacacc aacctcggca gccgtaaagc acatcaaatc gaaaataatc
     3241 cgcgcgttag cctgctgttc ccgtggcata cccttgagcg ccaggtgatg gtgatcggta
     3301 aagcagaacg actttcgact ctcgaagtga tgaaatattt tcatagccgc ccgcgtgata
     3361 gccagattgg tgcatgggtt tcgaagcagt ccagtcgcat ttctgcccgc ggtatccttg
     3421 aaagtaaatt cctggagctg aagcagaagt ttcaacaggg cgaagtgcca ttgccgagct
     3481 tttggggcgg ttttcgcgtc agccttgaac agattgagtt ctggcagggt ggtgagcatc
     3541 gcctgcatga ccgctttttg taccagcgtg aaaatgatgc gtggaagatt gatcgtcttg
     3601 caccctgaaa agatgcaaaa atcttgcttt aatcgctggt actcctgatt ctggcacttt
     3661 attctatgtc tctttcgcat ctggcgaaaa gtcgtgtacc ggcaaaggtg cagtcgttat
     3721 atacatggag attttgatgg caagcagtaa cttgattaaa caattgcaag agcgggggct
     3781 ggtagcccag gtgacggacg aggaagcgtt agcagagcga ctggcgcaag gcccgatcgc
     3841 gctctattgc ggcttcgatc ctaccgctga cagcttgcat ttggggcatc ttgttccatt
     3901 gttatgcctg aaacgcttcc agcaggcggg ccacaagccg gttgcgctgg taggcggcgc
     3961 gacgggtctg attggcgacc cgagcttcaa agctgccgag cgtaagctga acaccgaaga
     4021 aactgttcag gagtgggtgg acaaaatccg taagcaggtt gccccgttcc tcgatttcga
     4081 ctgtggagaa aactctgcta tcgcggcgaa caactatgac tggttcggca atatgaatgt
     4141 gctgaccttc ctgcgcgata ttggcaaaca cttctccgtt aaccagatga tcaacaaaga
     4201 agcggttaag cagcgtctca accgtgaaga tcaggggatt tcgttcactg agttttccta
     4261 caacctgttg cagggttatg acttcgcctg tctgaacaaa cagtacggtg tggtgctgca
     4321 aattggtggt tctgaccagt ggggtaacat cacttctggt atcgacctga cccgtcgtct
     4381 gcatcagaat caggtgtttg gcctgaccgt tccgctgatc actaaagcag atggcaccaa
     4441 atttggtaaa actgaaggcg gcgcagtctg gttggatccg aagaaaacca gcccgtacaa
     4501 attctaccag ttctggatca acactgcgga tgccgacgtt taccgcttcc tgaagttctt
     4561 cacctttatg agcattgaag agatcaacgc cctggaagaa gaagataaaa acagcggtaa
     4621 agcaccgcgc gcccagtatg tactggcgga gcaggtgact cgtctggttc acggtgaaga
     4681 aggtttacag gcggcaaaac gtattaccga atgcctgttc agcggttctt tgagtgcgct
     4741 gagtgaagcg gacttcgaac agctggcgca ggacggcgta ccgatggttg agatggaaaa
     4801 gggcgcagac ctgatgcagg cactggtcga ttctgaactg caaccttccc gtggtcaggc
     4861 acgtaaaact atcgcctcca atgccatcac cattaacggt gaaaaacagt ccgatcctga
     4921 atacttcttt aaagaagaag atcgtctgtt tggtcgtttt accttactgc gtcgcggtaa
     4981 aaagaattac tgtctgattt gctggaaata atgcattaag tggaaagggg gagtgagaaa
     5041 tcactccccc tggtttttat acagggaaca tgatgaaaaa tattctcgct atccagtctc
     5101 acgttgttta tggtcatgcg ggtaacagtg cggcagagtt tccgatgcgc cgcctgggcg
     5161 cgaacgtctg gccgctgaac accgttcaat tttctaatca cacccaatac ggcaaatgga
     5221 ctggctgcgt gatgccgccc agccatttaa ccgaaattgt gcaaggcatt gccgccattg
     5281 ataaattaca cacctgtgat gccgtattaa gtggctatct gggatcggcg gagcagggtg
     5341 aacatatcct cggtatcgtc cgtcaggtga aagccgcgaa tccgcaggcg aaatattttt
     5401 gcgatccggt aatgggtcat ccggaaaaag gctgtatcgt tgcaccgggt gtcgcagagt
     5461 ttcatgtgcg gcacggtttg cctgccagcg atatcattgc gccaaatctg gttgagctgg
     5521 aaatactctg tgagcatgcg gtaaataacg tcgaagaagc ggttctggca gcgcgcgaac
     5581 tcattgcgca agggccacaa attgtgttgg ttaaacacct ggcgcgagct ggctacagcc
     5641 gtgaccgttt tgaaatgctg ctggtcaccg ccgatgaagc ctggcatatc agccgtccgc
     5701 tggtggattt tggtatgcgc cagccggtag gtgttggtga tgtgacgagc ggtttactgc
     5761 tggtgaaact gcttcagggg gcaacgctgc aggaggcgct ggaacatgtg accgctgcag
     5821 tctacgaaat catggtgacc accaaagcaa tgcaggaata tgagctgcaa gtggtggctg
     5881 ctcaggatcg tattgccaaa ccagaacatt acttcagcgc aacaaagctc tgaaatatct
     5941 catttcggcc cacaacgacc tgtgggccga tggctattac tttaagcctt ccgctgacag
     6001 cgcgtcttgt acttccggac gttcagccat acgttgcata aatgctgcaa tgtgctctaa
     6061 cccttccaga ttcagtttca ccgcgtatgc ccagcgcaga accgtaaaca gataggcatc
     6121 agcaattgta aatctttgcc cgcagatcca gtgctcatcc ttcagtgcct cgttcacata
     6181 ttgcagcttc ttctccagct gcgcgcgaac tgtcggtttg tactcttccg gtgtatcagg
     6241 gcgaaacaga ggtgtgaaac ctttatgcag ctcggtggcg atgtaattca gccattcgat
     6301 ggttttatag cgggaaatac tgtttaccgg tgccagcaac tggcggtcgg ggacgctgtc
     6361 ggcaagatac tgcataatcg ctacgccttc cgtcagcaaa gtaccgtcat ccagcagcaa
     6421 tgcaggcacc tgccccttag ggttaacggc aaagtaatcg tcaccgtttt cgagacgttt
     6481 tttcattaaa tccacactga cgagggtaaa atcctttccg ctctcacgca gggtgatatg
     6541 ggaagcgaga gagcaggcac ccggtttgta gaacaatttc atcggtaact ccttttgtct
     6601 gtggtttaaa gtatggtagt gcgttgctga acaaaaaaaa agccgctaac gaaaagttag
     6661 cggcttgtaa aagagtttcc ctgaatttta cgctacggct gctttcgccg ctttgtctgc
     6721 agcgtcatcc tgcgtcatgc ggtgcagttt cggcgcggtc agcagcatca gtactgcaat
     6781 aacggcagta gcgacaccaa tctgcaagaa tacgcgacca tagacttcca gtgacatcag
     6841 cggatcggta acgttatccg gcacagccat catacccgca acataaccac caatcaggtt
     6901 tgcaccggca gtggtcagga accagctacc cataatgaag cccatcagac gctgcggaac
     6961 gagttgagca accattgcca gacccagacc agagatcatc agttccccga tgctctgcag
     7021 gccatagctt gcgaccagcc agcttacaga cacgatacca gcgtcagacg cgaatttcgc
     7081 acccagcggc agaatcagga acgcaccaga acacatcacc atgccgattg caaacttggt
     7141 tggcatcggc agggtatcgc ccatcttgtt atagatagcg gccagaatcg gactaccgat
     7201 gatgatccag aacgggttca gtgcctgata ctgctcaggt tctacggcca gacccagaat
     7261 ggagtgctca acgttacgaa tcgcaaagaa gttcagtgac gttggcatct ggctgtacag
     7321 cacgaagaag ataatggctt cgagcatcag gatgaaggca acgatcattt tacgacgcgc
     7381 agcacctttc atcgcgaagg cttctttacc gaagataacc acgataccga aggcaacaac
     7441 gcccagcgcc atacgcgcaa cttcctgatt gtgcagcagc caggtggcga tagcgatcag
     7501 tgccacaaca ccaataatgg tcagcagcag gttacggtag ttgataggct cgaagtctgg
     7561 ttttgaaccg tactgtttaa cccagcgttg gcagaaggcg aagttaacga tagtgatcag
     7621 caggcctaca acgctcaacg caaaggcaac actccagccg tatttcgcgg ccagccacgg
     7681 cgtagcaatc atagagaaga aagagccgat gttgacggac atgtagtaca tggtgaatgc
     7741 accgtccaga cgcgggtcgt ttttctcata gcatgtagaa agcagagaag acgggttagc
     7801 tttaaacagg ccgttaccga ccgcaatagc cgccataccc atataaacga taccggcgtc
     7861 gtgaccagac caggcaacca gagcataacc aatcgccagc acaatagcgc cgagcataat
     7921 tacgcgttta gtacccagta ccttgtcacc taaccagccg ccgatagcga ccagaccata
     7981 aaccagggca ctaaaggaag agaaaagggt gattgaatcc gcttcagaca tacccagttg
     8041 tttaaccagg taaacagcca taattccttg taggccgtaa taaccaaaac gttcccataa
     8101 ctcaatcgag aagatgagat agaacgcctt cggttgtttg aaagcgttca aactgacgct
     8161 ttcagttggt ttttggtttg cagtggacac ttttacctct ttttttacat cccatattaa
     8221 cgggggtgct ttctcgcgtt atgtataaaa aacatacgcg caattgttat agtgggaggg
     8281 gaaacggcag gtaatgttca ctatcttgca ctttcagaca agtcctttgt aataatctgt
     8341 tacatataac tgaggcgggg aattcaacca ttccgggaaa tgttatccag cgttaaattt
     8401 ttcaaaaaac gtaacggcag attttttcac tgcactgaga ggggggtgat ggcttaacgg
     8461 cgatatgttc tgctatttag cgtttttact agtgagatag tccagtttct gaaaaatagc
     8521 cagtgtaatg ttttgtaggt caatatttgt tgcgtgtgta aatttaaatg attttaacct
     8581 gattcttgca atgtttttac attgcgacag catcgtgatc ttgatcacga atgaatagaa
     8641 aatttcgcta ttaaaaaaac aattaacctg catttttggt tatccgcaaa cggattacct
     8701 gatgataacg ctaagcaatg gcatcattga cgttaaaaag atgttttaaa agaggataaa
     8761 gaaaggttat caatggggta atcggtgtta ccccttttct tcagatgtca actttctctt
     8821 tgtattcaca aagatcttca ataatacaag agccacagcg gggcttgcgg gcaatgcagg
     8881 tataacgccc gtgcaggatc aaccaatggt ggcagtcgac tttaaactct gccggaacca
     8941 ctttcagtag cttttcttct acctgttcga cgtttttccc cggcgcaaat tgagtacgat
     9001 tacaaacgcg gaaaatgtgc gtgtcgacag caatagtcgg ccagccgaat gcagtgttta
     9061 atacgacgtt ggctgtttta cgacctacgc cgggcagggc ttcaagcgca gcacgatctt
     9121 ccggaacctc gccattatgc tgctccagca agatacggca ggttttgatg atattttctg
     9181 ctttgctgtt ataaagccca atcgttttga tataggtttt caccccttca acgcccagtt
     9241 caagcatcgc tgcaggcgta ttcgccaccg ggtagagttt cgccgtcgcc ttattaacac
     9301 tgacatcggt cgcctgagcg gaaagcagta cggcaatcag caattcaaaa ggcgaactga
     9361 aattaagctc ggtggtggga tgaggattgt tctcacgcag gcgagtgagg atctccaggc
     9421 gttttgcttt attcatcaga cattccctgt ttcaccgttt ggcaatgcac gttctgcagc
     9481 tgcttcagca cggcgctttt tcattctttc atcaatcagg tattttcctg ccagcatcag
     9541 tcccaggcca ataaatgcac ctggtggcag catcgccagc aggaaagggg agtcggtgtg
     9601 gaaaatctcc acgcgtaata cttttgccca gctacctaac agcgcatctg caccgtcaaa
     9661 caacgtgcca ttgccgataa tttcgcgtag tgaacccagc acgaacatgg cgcaggttgc
     9721 gcccatacca attgaaaagc cgtccagtgc cgaaagcgcc ggaccttttt tggcggcgaa
     9781 ggcttcagcg cggcccacca cgatacagtt agtgacaatc agcggaataa aaatccctaa
     9841 tgattgatac aggccaaagg cgtaggcgtt gatcagcatc tgtacagcgc tgaccaccga
     9901 ggcgatgatc atcacgtaaa tgggaatgcg gatctcggct ggcgtccagt gacgcagcgt
     9961 cgaaatggtc aggttagtca gcgtcagtac cagcgtagtc gcaagtccta aacccagagc
    10021 gttagtggca gtggacgtga ccgccaatag cggacagagg ccgagcagct ggaccagcgc
    10081 agagttgttt ttccacaacc cctgaacaat aacgtcttta atttcgctca cgctttattc
    10141 tccacaggca ggaagttgag aaagttgtga cggtaacgtc tgagcgtaca atccggcgcg
    10201 ttttaccgca ttaaccaccg cgcggggagt aatcgttgcg ccggtgaact ggtcgaaatc
    10261 accaccatct ttcttcaccg cccaatgcgc atcatccgca ccactgattt ttttacccgc
    10321 aaaatgggtg atccagtcag aaaggcgcag ttcgatttta tcgccaagcc ctggcgtttc
    10381 gtggtgctct gtcacgcgcg tgccaagtac cgtgccgtta aaatcggctc ccaccagcag
    10441 ctgaatcgca ccggaatagc catctggcgc ggttgtttcc agaacggcgg ctaccggttt
    10501 gtcattctgt ttggcgatgt aaacccgatg ctcaccttta cctaactctg gcgcagttac
    10561 cagatagcaa ctctgtgcca gcgcattgtt atagcgttcg gctggcagca cctgatcaaa
    10621 taacgccttt tgttgcagac tggcctgttc agcaatcgtc gttttggtca tctggttgat
    10681 ggccgcagtt aaccctgttg aacccgctgc aaatagcgcc aacgtaatgc cgtgttttcg
    10741 gatagttttc agcatggttt accctttgcg atggccgtag acgcgcggac gcgtgtagta
    10801 atcgatcaga ggaaccgtga tgttcgccag caggacggca aaagccacgc cgtcaggata
    10861 gccgccgaaa ctgcggatca accagactaa taagcccgcc agcgcgccga aaataagacg
    10921 accacgattg gtcgtagaag cggtaaccgg gtcagtcaaa ataaagaatg cgccgagcat
    10981 ggtcgcacca gacagcagat gaatttgcgg tgctgccagt gtttctggtg agaacaacca
    11041 gcccaacgtt gcgcataacg ccagcgttac taagaagctg aggggaatat gccagcgaat
    11101 cgctttctgc catagcaacc acaggccgcc agccagccag gcgagattta cccattgcca
    11161 accagcgccc gccagaatac cgctgtagat cggatattgc ataatctgtt caaccgaatg
    11221 accggcacgg acagaggttt taaatgtatc cagcggtgtc gcctgactaa tgccatcaat
    11281 acctaagcgt agtgtgttca tatcaccacc actggcggta tgtccgctga aaataacctg
    11341 tatggcgtcg ataaaaccag ggatgttgac cgcaatttca tgtggcggta accagctggt
    11401 catctgcaca gggaaggaga tcagtaagac cacataacca atcattgccg gattaaacgg
    11461 gttttgcccc agaccgccat acaactgttt agcgatgatc accgcaaaca ccgtacccag
    11521 cacgaccatc caccatggcg cgaggggggg aatacttacc gccagcaata agcctgtcag
    11581 caatgctgag ttatctttca acgttgcggc taccgactgc ttgcgtagtt tgagtacgag
    11641 agcttcggct aacagagcac taaccgatgc caacaggatc tgaacgagag taccccaacc
    11701 aaaaaaccac agttgcgctg cgattcctgg cacggctgcg agcaacacca acagcataat
    11761 gcgcgatgtc tggcgctggt tatgggtata aggggagcta gctattctga ataccattta
    11821 gtcctcgtat acaaccttct gctgggcggc ttttttggcc tgagcgcggg caatagccgc
    11881 ggcaactgcc gctttgcgcg gatcaacctg ttcttctggt accgcattag cctgttgctg
    11941 ttccagcttg cgtgctttgg cgcgtgcaat agccgcttcg acggcagctt tgcgcggatc
    12001 aacctgttct tctggctccg cattagccgg ttgctgttcc cgtttgcgcg ctttggctcg
    12061 ggcaatagcg gcttcgacgg cggctttgcg cggatcgacc tgttgttctg gctcggcatt
    12121 agcctgttgc tgttccagct tgcgtgcttt ggcacgggca atagcggctt cgacggcggc
    12181 tttgcgcgga tcgacctgtt cttctggttc cgcattagcc tgttgctgtt ccagcttgcg
    12241 cgctttggcg cgggcgatag ctgcttcaac ggcagtttta cgtggatcag caacggttgc
    12301 tgcgtcgtta gtttgctgca gttctgcctg tttcgctctg gcttgcgctt tacgggcttc
    12361 ccgtgctgca ataattgcac tgttatccgg gcgttcgccc gctttaatca caataggctg
    12421 tgtagcctgg gcctgtttct ctttcacccg cgccagagcg gcagcaatcg catctttatc
    12481 tttggttgca ggttgaacgg ctgcgctctt atgtcgttca aggcgagccg ctttttcgcg
    12541 ctccagacga gcctggcgcg cttcgaaacg cgctttggct tctgcggcgc gcttttcttc
    12601 ctgacgaata gccgcaattt cagctttttc ctgacggaaa tattgcacca ggggaatatt
    12661 gctcgggcaa acccacgcgc aagccccaca ttcaatgcaa tcagcaatgt tatgcgtggt
    12721 agctttatcg tgttgctgac ctttgctgaa ccagtacaac tgttgcggca aaagatccgc
    12781 agggcaggcg tcagcacagg cgctacaccg gatgcagctt tgttcttcct gtggttcgcc
    12841 aagttcattg gcagagggag ccaacagaca gttggtaatc tttacgaccg ggacatccag
    12901 ccatggcaag gtaaagccca ttagcgggcc acccataatc accatttgat cggcagaggg
    12961 gcagaatccg gcatcattca ataaatgacg cactggcgtc cccagccgtg cccagacgtt
    13021 gcccgggcga gcgattgctt cgccagtcag ggttacaaca cgctcggtaa tcggctcgcc
    13081 atcaataacg gcacgtttca ctgcataagc agtgccgacg ttttgcatta atacgccgat
    13141 atcggatgaa cgcccgccat gtggaacctg cttcccggtc agaatgtagg ttaattgttt
    13201 agcaccgcca gaaggatatt tggttggaat cacccgcaga gaaatatcgt tagagtccgc
    13261 cagcaccgcg cgcagcatgg aaatcgcctg cggtttgtta tcttcaatgc cgataagaat
    13321 ttcgcgtggc tgcagaatat gcgcaagaat gcgaataccc tctacgacct gagccgcgca
    13381 atcctgcatc aaacggtcat cggcggtaat gtacggctcg cactcagccg cgttgataat
    13441 caacgtttca atcttatctc cgccaccctg caatttaacg cctgtcggga atcctgcacc
    13501 gcccagcccg gcaacaccaa actgatgtat gcgctcgatt aactcttcgc gactgcgagt
    13561 gcgataatcg gcccagccgt cgcgcgggat ccagcagtct tcaccatcgg catcaataat
    13621 cacgcttaat tcagctaaag ctgaaggatg agccgtagag tggggcgcaa tagccgtaac
    13681 ggtacccgag gtgggcgcgt gaacaggcag cattttgccg cgaccacggg taagcggctg
    13741 gccgcgcaat actttatcgc cgacgctaac gcacaactca ccttcagcgc caatatgctg
    13801 tttcagtgga ataacaaaac gctgcgccag gggtacctgg cgcaggggtg taccgttgga
    13861 ctgggttttc atctccggtg gatggatgcc gccgttgaaa tcccagattt tattttttct
    13921 gaatgcagag aataacttaa gcatggtgtt ccacgggaat gatacgcacg ggaatggtgt
    13981 tcagatccca tttccaggag tcaggtgttt ctgcgaccgg ttgcaacgag atgcagtgcg
    14041 tcgggcacgg atcaacacat aaattgcagc ccgtacagag atcactcatt accgtatgca
    14101 tggcacgggt agcgccaacg atggcgtcta ccggacacgc ctgaatacat ttagtgcagc
    14161 caatacagtt attttcatca ataaccgcca ccatccgcgc aggcgttatc tcttgcgctt
    14221 cgccatccag cggctgcggc tcgacattaa gcaactcggc aatttttagc atcacagctt
    14281 cgccacctgg ggcgcaacgg ttgatttttt caccgttaca gctgatggct tccgcgtagg
    14341 ggcgacagcc gggataaccg cactgaccac actggctctg cggtaagatt tcgtcaattt
    14401 tctcaacgac cggatcgtct tccaccgcaa aacggcggga ggcataaccc agaatggcgc
    14461 caaacgccag gcccagcagg ctcacggcgg caacggcaat ccagatagca ttcattacaa
    14521 cttcaccaaa ccactaaagc ccataaaggc cagagacata agacctgcgg taattaacgc
    14581 aatggcatta ccgcgaaaag gtgccgggac atcagccaca gcaaggcgtt cgcggatggc
    14641 ggcgaagagc accatcacca gcgagaaacc gacagcggcg gaaaaaccgt acagcgccga
    14701 ctgcaagaaa ttgtgcccga gattgatatt cagcaacgcc acgccgagca ctgcacagtt
    14761 ggtggtgata agcggcaaaa aaatccccag cagacggtaa agcaccgggc tggttttgcg
    14821 caccaccatc tcggtgaact gcacgaccac agcaatcacc agaataaatg ccagggtgcg
    14881 caggtaaatc agattaagtg ggatcaaaat ccacgtatcg ataagccagg cgcaaataga
    14941 cgccagcgtc atcacaaacg ttgttgccag ccccatgccc atcgcggttt ccagtttttt
    15001 ggaaaccccc ataaacggac agagaccgag aaacttgacc agtacaaagt tattgaccag
    15061 tacagttccg acaaacagta acaggtagtc agtcattttt cggcctgaaa taaaaaagcc
    15121 gcctattatc cgttaatcca gagcaggcga caacaggtta actgtaaggt tattacggat
    15181 tcacaaacgt cgcttttacc cgcgacgagc gtttgaaata ggggacgatc aatgcggttg
    15241 ccaacaaagt aaacagcaac tggcgaacgg ctatgccatc ttccacgggt gaaaaagcga
    15301 atgcttttac tgccagcaat acggaaataa gcagccagat aatatagtgt ttaggaacac
    15361 aacgccgacg tttaaagaaa gcgatggtca accacagtgt gtaataccac atggcgatag
    15421 cggtaataaa agaaacgccc cataagattt gtgtcgtcag tgcttgtccg ccgagcgttt
    15481 gaaatgtttg cggagaagat aacgcagcgg tatacagcaa caacgccagc gtcgtactca
    15541 acagagcgac taaaagccag gccaacgggc caagcaacca gcctccaata cgttgtggcg
    15601 ttgtggtggt cataggttct ccagaatagc gacgcaaaca ggcaaatcta attttagggg
    15661 cgttattata aggcttttat aaccgatcgc tactttcttt attggacata gtgccagacg
    15721 gacttcggta cctggccgag gtcaaacaaa cgcccgccag aaaccagctc tgcgcgacgg
    15781 tgatcggcag cacgatacat attgatcagt tcctgatcgt ccgtcagggt gtaattaaga
    15841 tggtcgtaga gtttttccag actttcgagt gaactgattt tgcgaaattt taataagtag
    15901 tcctgaacag tcat
//
LOCUS       DAPHBJ010000096        15894 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.097, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000096 DAPHBJ010000000
VERSION     DAPHBJ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.097"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..3057)
                     /gene="lacZ"
                     /locus_tag="RHZ76_002724"
     CDS             complement(<1..3057)
                     /gene="lacZ"
                     /locus_tag="RHZ76_002724"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDV2813339.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQESRSLNGEWRFAWFPAPEAVPESWLERDLPDADTVIVPSNWQMHGYDAPIYT
                     NVTYPIAVNPPYVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLHAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEADVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADHVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDHYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTFATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFSLSGRTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATTWSAAGHISAWQQWRLAENLSVTLPSAPHAIPQ
                     LTTSETDFCIELDNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTVHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASNTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFHLSAGSYHYQL"
     gene            complement(3179..4261)
                     /gene="lacI"
                     /locus_tag="RHZ76_002725"
     CDS             complement(3179..4261)
                     /gene="lacI"
                     /locus_tag="RHZ76_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HDV2813340.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAQ
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIHPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLKLSQGQAVKSNQLLPVSLVKRKTTLAPNT
                     QTTSPRTLADSLMQLARQVSRLESGQ"
     gene            4614..5623
                     /locus_tag="RHZ76_002726"
                     /pseudo
     CDS             4614..5623
                     /locus_tag="RHZ76_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152284.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
     gene            6027..7031
                     /locus_tag="RHZ76_002727"
     CDS             6027..7031
                     /locus_tag="RHZ76_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS4321 family transposase"
                     /protein_id="HDV2813341.1"
                     /translation="MENIALIGIDLGKNSFHIHCQDRRGKAVYRKKFTRPKLIEFLAT
                     CPATTIAMEACGGSHFMARKLEELGHSPKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISR
                     LSTILEDNSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLKRKLDEDEVGQRLLSIPCV
                     GTLTASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLL
                     VQCARVFIQKLEHQSGKLADWVRDLLCRKSNFVVTCALANKLARIAWALTARQQTYVA
                     "
     gene            7293..7571
                     /locus_tag="RHZ76_002728"
     CDS             7293..7571
                     /locus_tag="RHZ76_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDV2813342.1"
                     /translation="MPSGPHIEWLTVLDFDEIKIDRIFIANIDDPVKRALLVSVVKGL
                     RGTGKPLVFEGVETPGQFEFVRSLGPGYLVQGWYTGKPETISAMNIQG"
     gene            complement(7600..7827)
                     /locus_tag="RHZ76_002729"
                     /pseudo
     CDS             complement(7600..7827)
                     /locus_tag="RHZ76_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(7902..8195)
                     /gene="tnpB"
                     /locus_tag="RHZ76_002730"
     CDS             complement(7902..8195)
                     /gene="tnpB"
                     /locus_tag="RHZ76_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV2813343.1"
                     /translation="MRNGFNGLAAKVQTMLKDDPISGHVFIFRGRSGSKVKLLWSTGD
                     GLCLLTKRLERGRFAWPSARDGKVFLTPAQLAMLLEGIDWRQPKRLLTSLTML"
     gene            complement(8294..9061)
                     /gene="fecE"
                     /locus_tag="RHZ76_002731"
     CDS             complement(8294..9061)
                     /gene="fecE"
                     /locus_tag="RHZ76_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HDV2813344.1"
                     /translation="MRLRTENLTVSYGAQTVLDGLSLALPAGKITALLGPNGCGKSTL
                     LNCFSRLLTPDSGEILLDEKPIAGFSARQLARRLALLPQHHLSPEGITVRELVSYGRS
                     PWLSLWGRLSAEDNERVNVAMSQTRTRNLADRRLTQLSGGQRQRAFLAMVLAQDTPLI
                     LLDEPTTYLDINHQVELMRLMVELKRQGKTVVTVLHDLNQASRYCDHLVVLASGRVMA
                     QGAPEAVMKPELLKTVFSVEAEIHPEPVSGRPMCVVK"
     gene            complement(9062..10018)
                     /gene="fecD"
                     /locus_tag="RHZ76_002732"
     CDS             complement(9062..10018)
                     /gene="fecD"
                     /locus_tag="RHZ76_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HDV2813345.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQDGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLVQGIVRNPLASPDILGINHAASLASVGALLL
                     MPSLSVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLLH
                     PQDVNSALLWLTGSLWGRDWHFVKIAVPLLILFLPLSLRFCRDLDLLALGDARAATLG
                     VSVQRTRFQGLMLAVAMTATGVAVCGPISFIGLVVPHMVRRIAGGRHRWLMPVSALTG
                     ALLLVIADLLARIIHPPLELPAGVLTAIIGAPWFVWLLVRMR"
     gene            complement(10015..11013)
                     /gene="fecC"
                     /locus_tag="RHZ76_002733"
     CDS             complement(10015..11013)
                     /gene="fecC"
                     /locus_tag="RHZ76_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HDV2813346.1"
                     /translation="MTALKHPVLLWGLPVAVLIIIFWLSLFCYSAIPVSGADAIRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPVAGYSLSFIAACGGGVSWLLVMTAGGGFRHTQDRNKLILAGIALS
                     AFCMALTRITLLLAEDHAYGIFYWLAGGVSHAHWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNVSDSTAHTLGVNLPRLRLIINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLMADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(11010..11912)
                     /gene="fecB"
                     /locus_tag="RHZ76_002734"
     CDS             complement(11010..11912)
                     /gene="fecB"
                     /locus_tag="RHZ76_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HDV2813347.1"
                     /translation="MLAFIRFLFAGLLLVIGHAFAATVQDEHGTFTLDKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGIYTALQQIAPVLLLKSRNETYAENLHSAAIIGEVVGKKREMQARLEQH
                     KEKMAQWSSQLPTGTRVAFGTSREQLFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQILTAAQKQQVASVDSNAWARMRG
                     IFAAERIAADTVKIFHHQPLTDVK"
     gene            complement(11957..14281)
                     /gene="fecA"
                     /locus_tag="RHZ76_002735"
     CDS             complement(11957..14281)
                     /gene="fecA"
                     /locus_tag="RHZ76_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HDV2813348.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVDIAPGSLD
                     KALNQYAAHSGITLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLRNNSW
                     TLETAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPNEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQSFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAIKGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSVYASYAYVNAEIREKGDTYGNQVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(14368..15321)
                     /gene="fecR"
                     /locus_tag="RHZ76_002736"
     CDS             complement(14368..15321)
                     /gene="fecR"
                     /locus_tag="RHZ76_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HDV2813349.1"
                     /translation="MNPSLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGAVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDAQQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKGILSFSDKPLGEVIATLSRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(15318..15839)
                     /gene="fecI"
                     /locus_tag="RHZ76_002737"
     CDS             complement(15318..15839)
                     /gene="fecI"
                     /locus_tag="RHZ76_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HDV2813350.1"
                     /translation="MSNRATSTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMSSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGVAPSPE
                     ERESQLETLQLVDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSVSSVKKYVAKA
                     VEHCLLFRLEHGL"
BASE COUNT         3483 a         4412 c         4478 g         3521 t
ORIGIN      
        1 caactggtaa tggtagctac cggcgctaag gtggaattcc gccgacactg acgggctcca
       61 ggagtcgtcg ccaccaatcc ccatatggaa accgtcgata ttcagccatg tgccttcttc
      121 cgcgtgcagc agatggcgat ggctggtttc catcagttgc tgttgactgt agcggctgat
      181 gttgaactgg aagtcgccgc gccactggtg tgggccataa ttcaattcgc gcgtcccgca
      241 gcgcagaccg ttttcgctcg ggaagacgta cggggtatac atgtctgaca atggcagatc
      301 ccagcggtca aaacaggccg cagtaaggcg gtcgggatag ttttcttgcg gccctaatcc
      361 gagccagttt acccgctctg ctacctgcgc cagctggcag gtcaggccaa tccgcgccgg
      421 atgtggcgta ttgctcgcca cttcaacatc aacggtaatc gccatttgac cacttccatc
      481 aatccggtag gttttccggc tgataaataa ggttttcccc tgatgctgcc acgcgtggac
      541 cgtggtaatc agcaccgcgt cggcaagcgt atctgccgtg cactgcaaca acgctgcttc
      601 ggcctggtaa tggcccgccg ccttccagcg ttcgacccag gcgttagggt caatgcgggt
      661 cgcttcactt acgccaatgt cgttatccag cggtgcgcgg gtgaactgat cgcgcagcgg
      721 cgtcagcagt tgttttttat cgccaatcca catctgtgaa agaaagcctg actggcggtt
      781 aaattgccaa cgcttattat ccagctcgat gcaaaaatcc gtttcgctgg tggtcagttg
      841 cgggatggcg tggggcgcgg aggggagtgt cacgctgagg ttttccgcca gacgccactg
      901 ctgccaggcg ctgatgtgtc cggctgctga ccatgtggtc gcgttcggtt gcactacgtg
      961 aaccgttagc cagagttgtc cggcgctctc cggctgcggt agttcaggca gttcaatcaa
     1021 ctgtttacct tgtggagcga catccagagg cacttcaccg cttgccagcg gcttgccatc
     1081 cagcgccacc atccagtgca ggagctcgtt atcgctatga cggaacaggt attcgctggt
     1141 cacttcgatg gttcgcccgg ataaactgaa ctggaaaaac tgctgctggt gttttgcttc
     1201 cgtcagcgcc ggatgcggcg tgcggtctgc aaagaccaga ccgttcatgc agaactggcg
     1261 atcattcggc gtatcgccaa aatcaccgcc gtaagccgac cacggattgc cattttcatc
     1321 atatttaatc agcgactgat ccacccagtc ccagacgaag ccgccctgta aacgggggta
     1381 ctgacgaaac gcctgccagt atttagcgaa gccgccaaga ctgttaccca tcgcgtgggc
     1441 gtattcgcaa aggatcagcg ggcgcgtctc tccaggcaac gaaagccatt ttttgatgga
     1501 ccatttcggc acagccggga agggctggtc ttcatccacg cgcgcgtaca tcgggcaaat
     1561 aatatcggtt gcgaaggtgt cggctccgcc gccttcatac tgtaccgggc gggaaggatc
     1621 gacagatttg atccagcgat agagtgcgtc gtgattagcg ccgtggcctg attcattccc
     1681 cagcgaccag atgatcacac tcgggtgatt acgatcgcgc tgcaccattc gcgttacgcg
     1741 ttcgctcatc gcgggtagcc agcgcggatc atcggtcaga cgattcattg gcaccatgcc
     1801 gtgggtttca atgttggctt catccaccac atacaggccg tagtggtcgc acagcgtgta
     1861 ccacagcgga tggttcggat aatgcgaaca gcgcacggcg ttaaagttgt tctgcttcat
     1921 cagcaggata tcctgcacca tcgtctgctc atccatgacc tgaccatgca gaggatgatg
     1981 ctcgtgacgg ttaacgccgc gaatcagcaa cggcttgccg ttcagcagca gcagaccatt
     2041 ttcaatccgc acctcgcgga aaccgacatc gcaggcttct gcttcaatca gcgtgccgtc
     2101 ggcggtgtgc agttcaacca ccgcacgata gagattcggg atttcggcgc tccacagttt
     2161 cgggttttcg acgttcagac gtagggtgac gtgatcggca taaccaccac gctcatcgat
     2221 aatttcaccg ccgaaaggcg cggtgccgct ggcgacctgc gtttcaccct gccacaaaga
     2281 aaccgtcacc cgcagctcat cgcgcagctc gccgtacatc tgaacgtctg cctccagtac
     2341 agcgcggctg aaatcatcat taaagtgagt ggctacatgg aaatcgctga tttgcgtgct
     2401 cggtttatgc agcaacgaga cgtcacggaa aatgccgctc atccgccaca tatcctgatc
     2461 ttccagataa ctgccgtcac tccagcgcag caccatcacc gcgaggcggt tttctccggc
     2521 gtgtaaaaat gcgctcaggt caaattcaga cggcaaacga ctgtcctgtc cgtaaccgac
     2581 ccagcgcccg ttgcaccaca aatgaaacgc cgagttaaca ccatcaaaaa taattcgcgt
     2641 ctggccttcc tgtagccagc tttcatcaac attaaatgtg agcgagtaac aacccgtcgg
     2701 attctccgtg ggaacatacg gcggattgac cgcaatggga taggtcacgt tggtgtagat
     2761 aggcgcatcg taaccgtgca tctgccagtt tgaggggacg atgacagtat cggcgtcagg
     2821 aagatcgcgc tccagccagc tttctggtac cgcttctggt gccggaaacc aggcaaagcg
     2881 ccattcacca ttcaggctgc gcgactcttg ggaagggcga tcggtgcggg cctcttcgct
     2941 attacgccag ctggcgaaag ggggatgtgc cgcaaggcga ttaagttggg taacgccagg
     3001 gttttcccag tcacgacgtt gtaatacgac ggccagtgaa tccgtaatca ttgtcatagt
     3061 tgtatcctgc gtgaattgtt atccgctcac attttcgcat aacatacgag ccggacacat
     3121 agagtgtaaa gcctggggtg cctaatgagt gagctgactc acattaattg cgttgcgctc
     3181 actgcccgct ttcaagtcgg gaaacttgtc gtgccagctg cattaaggaa tctgccaacg
     3241 tgcggggaga ggtggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga
     3301 cgggcaacag ctgattgctc ttcaccgcct ggccctgaga gagtttcagc aagcggtcca
     3361 cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac
     3421 acgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcaacc
     3481 cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg
     3541 cagtgggaac gatgccctca tttagcattt gcatggtttg ttgaaaaccg gacatggcac
     3601 tccagtcgcc ttcccgttcc gctatcggat gaatttgatt gcgagtgaga tatttatgcc
     3661 agcccgccag acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt
     3721 gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga
     3781 aaataatact gttgatggga gtctggtcag agacatcaag aaataacgcc ggaacattag
     3841 cgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc
     3901 cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc
     3961 gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg
     4021 cgacaatttg cgacggcgcg tgcagggcca gactggaggt agcaacgcca atcagcaacg
     4081 actgtttccc cgccagttgt tgtgccacgc ggttgggaat gtagttcagc tgcgccatcg
     4141 ccgcttccac cttttcccgc gttttcgcag agacgtggct ggcctggttc accacgcggg
     4201 aaacggtctg ataagagaca ccggcatact ctgcgacatc gtatagcgtt actggtttca
     4261 catttaccac cctgaattga ctctcttcgg gtgctatcat gccataccac gaaatgtttt
     4321 gcgccattcg gagggggcac gccgaagtga gattcaaaat gccgacgtca gttcacaggt
     4381 gggtaatgat accaacgtgt tgatttcgta ttttcactga caccaggatc atcctgatgt
     4441 tacaaggatt gaatgactac agattaaaat agtcatcaac aggttgacag cattacccag
     4501 cgcccgcttt ccgcgtattt ttcgatttgc actgccgggg gcactggcgg gtatcgcgtt
     4561 ccgcacactt ttacatttat ttatctggtg actgaccttt ttaaggagac agaatgaaag
     4621 ccgttggttt ctggcaaccc gggaaagtac atccgttatt gaagatttgt cgcttcccca
     4681 cccatccatg cccaaggggc gtgatctttt gatcagagtc agggcggtcg cggttaaccc
     4741 acgggacctg aaaagccggc gaaacatcag tccctctgca ggcaatccgg tagttatggg
     4801 atacgacgct tccggtgttg ttgaagcaac cggaccagag gtgacgctgt tcaggccagg
     4861 tgaccacgtt ttctatgccg gtgtgctcga tcgccaggga gccaacgcag agtttcagct
     4921 ggttgacgaa cgcattacgg ggataaagcc ggcagcacta agttttgaag ctgcagcgtc
     4981 ggttcctctg accggtctga cagcctggga aatgttattt gatcgtctgc agttgtcagt
     5041 gaacggagat gctgatcaga ccctgttgat gcttggcggt gcgggcggag tcccttgtat
     5101 ggctatacag cttgcccgca ctctgagtcg ggtgaacatc atcgggactg cctcccggca
     5161 ggagagtgtc agagcggttc gcgggtttgg tgcgcattat gtcctggatc attcatgtgc
     5221 gcttgcgcca caggttgcca gccttgctgg actccctccc attacccgga ttttctcaac
     5281 gtacaccacg ccatctgcct gggaaaatat ggccgaaatt attgccccac aaggccgtat
     5341 cggactcatc gacgaccctg aaccactgga tctgcgtttg atcaaattta agagcgtttc
     5401 tgttcactgg gaggcaatgt ttacccgccc catgcatgct actgcggaca tgtctaaaca
     5461 gcatgatatt cttaacaaac tctcatgcat gctggattcc ggtaagctca aacctccgtt
     5521 gctggcagag tatggcaggc tgaatgctaa agcacttcgc cgggctcatg aggacatgga
     5581 aaataaaaat gtgaacggga aaattgtttt ttcagggttc tgatgaacag gcctgtcaga
     5641 gtaaaaaaag attcgggttc agcacaatgc tgaattctgt ctggctatag agtaagaatc
     5701 gtaccggaat tgagcattta ccctcagtac ctgcggatga aaaagcagtc tgccctgaca
     5761 tactgaacaa tcccttctca taagatgcct ctcttttgct gtttaaagag ctgagcagtc
     5821 cgggcggaga ctgacctcgt tccgctttcc aagcgacccc cttgttgaaa gtgcgacaaa
     5881 agagataggg cgaatgacta tacttcgctc atagctgccc gccatgccag ctcaaaaaac
     5941 aggcttaacg taggatattt aatgagatgg tcactccctc cttcccggta ctatgctgag
     6001 gacaggcttt cattcggaga actatcatgg aaaacattgc gctcattggt atcgatctgg
     6061 gtaaaaactc tttccatatt cattgccagg atcgtcgcgg gaaggctgtt taccgtaaaa
     6121 aatttacccg gccaaagttg atcgaatttt tggcgacatg ccccgctaca accatcgcaa
     6181 tggaagcctg tggcggttct cactttatgg cacgcaagtt ggaagagttg gggcattccc
     6241 caaagctgat atcaccacaa tttgtccgcc cgttcgttaa aagcaataaa aacgactttg
     6301 tcgacgccga agctatttgt gaagctgcat cgcgtccgtc tatgcgtttt gtgcagccca
     6361 gaacggaatc tcagcaggca atgcgggctc tgcatcgtgt ccgtgaatcc ctggttcagg
     6421 ataaggtaaa aacaaccaat caaatgcatg cttttctgct ggaatttggc attagcgttc
     6481 cccgaggagc tgccgttatt agccgactga gtaccattct tgaggataat agtttgcctc
     6541 tttacctcag ccagttattg ctgaaattac aacagcatta tcactatctt gttgagcaga
     6601 ttaaagattt ggaatcccag ttgaaacgaa agttggacga agatgaggtt ggacagcgct
     6661 tgctgagcat tccctgcgtc ggaacactga cagcgagtac tatttcaact gagattggcg
     6721 acgggaagca gtacgccagc agccgtgact ttgcggcggc aacagggctt gtacctcggc
     6781 agtacagcac gggaggtagg acgacattgc tgggaattag taagcgaggt aataaaaaga
     6841 tccgaacttt gttggttcaa tgtgccaggg tattcataca aaaactggaa caccagtctg
     6901 gcaaattggc cgattgggtc agggatttac tgtgccggaa aagcaacttt gtcgtcactt
     6961 gtgctctggc aaacaagctg gccagaatag cctgggccct aacggcacga cagcaaactt
     7021 atgtagcata acggcagaaa tacaccggtt taaagaatta ctgatctggt tttgcgaata
     7081 ctgatattga tgatactaac ggcccaccgg cctgttgagg aacctgtaaa acggaaaggc
     7141 tcattgaagc cgtatatttt ctggaggttc atcaggcgcg gaactcatca aggcgcggga
     7201 ataaaatccc attcagacgc cggatagatt caagcaagcc aacttgtcgt caaaatcggt
     7261 gttgcaaaaa cgggagtgac catagattcc gtttgccaag cggcccccat attgaatggc
     7321 tgacggtact ggatttcgat gagataaaaa tagaccgtat ttttattgcc aatattgatg
     7381 acccggtgaa gcgagcgcta cttgtttctg tggtgaaagg gttacgtggt actggcaaac
     7441 cactggtgtt tgaaggggtg gaaacgccag gacagtttga attcgtccgt tctcttggcc
     7501 ccggttacct agttcagggc tggtataccg gaaaacccga aacaatatct gcgatgaata
     7561 ttcagggtta aaacgatacg gggcccggcc cgtatcggca acccgacccg tcagcgtatc
     7621 gctttctgtc tgaagccggt tcaggtcggc ttctatctgc gcgatgcggc gggagattta
     7681 ttcagaacga ctgccgaagt tcatccggcg aagcttatcc agctgcgcct gcagatggtc
     7741 tatttcgcgc tcccggtcgg cctgcttttc cagcagggcg cggttcagcg cttcctgttc
     7801 ggacaggcgc tgtttcagca gaaggatgtc gtcaggggga ggtgttgttc ataagcccgc
     7861 attttaccag gcttattcaa cgacaaccag gataaagagg cctacaacat ggtcagggac
     7921 gtaagcagtc ttttaggctg ccgccagtcg atgccttcca gaagcattgc cagctgcgcc
     7981 ggggtcagga acactttgcc atcgcgggca gagggccagg cgaagcggcc gcgttccagc
     8041 cgtttggtca gcaggcacag cccgtcgccg gtggaccaca gcagtttgac cttactgccg
     8101 ctgcggcccc ggaagatgaa gacatggccg gatatcgggt catctttcag catcgtctgc
     8161 acctttgcgg cgagaccatt gaagccgttg cgcatgtcgg tgataccggc aaccagccag
     8221 attttggtgc ctgttggcag gtggttatca cttcctttat ctgtcctgtg atgatcctta
     8281 tgacttcgcc agactacttc accacacaca tgggtctgcc agataccggt tcgggatgga
     8341 tttccgcttc tacgctgaac accgtcttta gaagctctgg tttcatcact gcttccggag
     8401 caccttgcgc catcacgcga ccgcttgcca gtaccaccag atgatcgcaa tagcggctgg
     8461 cctgattcag atcgtggagt accgtcacta cggttttccc ctgccttttg agttcaacca
     8521 tcaggcgcat cagttcaacc tgatgattga tatcgaggta ggtcgtaggc tcatcaagaa
     8581 gaatgagtgg ggtatcctgt gccagcacca tcgccagaaa cgcacgctgg cgctggccgc
     8641 cggaaagctg ggttaacctg cggtcagcca gattgcgcgt ccgggtctga ctcattgcca
     8701 cgttgacccg ctcgttgtct tccgcggaga gtcgccccca caacgacagc cacgggctgc
     8761 ggccgtagga aaccagttcc cgtacagtga tcccctcggg ggataaatgg tgttgcggca
     8821 gtaaagccag gcggcgagcc agctggcggg cggaaaaacc ggctatgggt ttttcatcaa
     8881 gcaatatctc gccagagtct ggtgttaaca ggcgagaaaa acagttcaac agcgtcgatt
     8941 tcccgcaacc gttagggccg agcagggccg tgatttttcc ggcaggcagg gcgagagaaa
     9001 gtccatccag cacggtttgt gcgccatagc tgacggtcag attttcagta cgtaatctca
     9061 tttatcgcat tctcacaagc agccagacaa accacggtgc gccgataatg gcggtcagca
     9121 cgcctgccgg aagctccagc ggcggatgaa tgattcgtgc cagcagatcg gcaatgacca
     9181 gcaacaacgc gcccgtcagg gccgaaacgg gcatcagcca gcggtgacgc ccgccggcga
     9241 tcctgcgcac catgtgcggc acgacgagac caataaaact tatcgggcca cagacggcca
     9301 cgccggttgc tgtcatcgcg acagccagca tcagcccctg gaaccgggtg cgctgaaccg
     9361 acacgccgag cgtggctgct cgtgcatcgc ccagcgccag caggtcgaga tcgcgacaaa
     9421 agcgcaggct cagcggcaaa aacagaatga gcaacggcac ggcaatcttc acaaagtgcc
     9481 agtcgcgccc ccataagcta cccgtcagcc acagcagggc actgtttaca tcctgcgggt
     9541 gcaggagcat caggtaatcc gtcaggctcg cccagcaggc ggaaagcgcc acgccggtga
     9601 gcgccagctt catcggctgg tgggtctttg ccagcatctt cagcaagatc aaccccgcca
     9661 taccgcccgc aaaggccagc agcggcagca ccatcacgga tagtgatggc ataagaagca
     9721 gtgctcccac agaggcaagg ctggcggcat ggttaatgcc gagaatatcc ggtgatgcta
     9781 gcgggttacg cacaatcccc tgtaccagca cgcccgccac ggcgagggct gcaccgacaa
     9841 acagtgccag cagcaagcgc ggcagtcggt actccatcaa tacataataa tgctcgcgtc
     9901 cgtcctgcca gtcggtcagc agcgcgcgcc acggcacggg gatcaccccc atatggagtg
     9961 ataacagcgc acaacctgcc agggcaaggg tgatgaaaat gaccagcgca attttcatcc
    10021 tcgcctcctc accagccaga caaagcaagg actgccaatc agcgccagca ctgcgcctgc
    10081 gggcagatcg ccggggaagg ccagcgcgcg tgcgagtaca tctgccatca gcatcagcgt
    10141 ggcccccagc agcatgctca ccggcagtac gttgcgctga tcgaagcctg cccagaagcg
    10201 cgccagatgt ggcaccagca ggccgataaa cgccaccgga cctgccacac tgacgcacgc
    10261 gccaaccaga agcagcacta acatattgat gatcaaacgt agtctcggca ggttcactcc
    10321 cagcgtatgg gcggtactgt cgctgacgtt gagcaggttc agttgattcg ccagcagcaa
    10381 cacgacaggg actgcagtga ccaccaccgg caagagctgc cagacatcct gccagtgggc
    10441 gtgggacact ccgcctgcca gccagtaaaa gatgccgtaa gcatgatctt cggccagcag
    10501 cagggtgatg cgggtcaggg ccatacaaaa ggccgacagc gcgatacccg cgaggatcag
    10561 tttgtttctg tcctgggtat gacgaaaccc gcctcctgcg gtcatgacca gcagccagct
    10621 cacgccgccc ccgcatgccg cgatgaacga cagggaatag cctgcaaccg gcgtcggact
    10681 cagcgcgctg gtaagcgcca tagccagcgc cgcgccgctg ttaatgccga gcagtgaagg
    10741 agaggccatt gggttgtggg tcagggtttg cagcagcgtg cccgcgagcg ccaggcttgc
    10801 gccgatcaga acggcgacca ggcttcgcgg caaacgaagg ttttgcacca gcgcttctgg
    10861 tagcgttggc gtgtgtccag gcagcagggc gcggattgcg tctgctccgg aaacaggaat
    10921 ggccgagtag caaaacagac tcagccagaa aataataata agtactgcaa cgggaagccc
    10981 ccacagcagc accgggtgtt ttagcgcggt catttcacat cggtaagcgg ctgatggtgg
    11041 aagattttta ccgtgtcagc ggcaatacgc tctgcggcaa aaataccgcg catccgcgcc
    11101 caggcgttac tgtcgactga agcaacctgc tgcttctgcg cggcggttaa tatctgccag
    11161 agcggatctt gttgccagcg tttaacaatg ctctcttcgc gatagtgggc aaccagcagc
    11221 caggcaggat tgaccgccag cagttgctcc aggccgatgg acggcatgga cgcgcccgcc
    11281 atcgcagcgg gaacgttcag ccccagagaa gccagcacgc tgccggtcca ggtctcctgg
    11341 gtatgcaggt tgaatagctg ttcgcgtgat gtgccaaagg ccacgcgtgt ccctgtggga
    11401 agctggctgg accactgcgc catcttctct ttatgttgtt ccagacgcgc ctgcatctct
    11461 cgttttttac ctaccacttc gccgatgata gccgctgagt gcaaattttc agcgtaggtt
    11521 tcgttgcggg acttaagcag cagtaccggc gcgatttgct gcaaggcagt gtaaatcccc
    11581 gcatggcgac tgctgtcggc aatgatcagg tctggtttca gggcagcaat ggcttccagg
    11641 ctcggctgcg cgcgcgttcc gacggactgc cacggtttca ggtgcgcacg cacttcgggc
    11701 aggatgcgtt ttgcatcgtt atcgtcggca ataccgatcg ggctgacgtc cacggcggcc
    11761 agcgcatcgg cgaacgagag ttccagcacc acaatccgtt gtggcgtttt atcgagtgta
    11821 aacgtgccgt gttcgtcctg aaccgtggcg gcaaaggcgt ggccgatcac cagcagcagg
    11881 cctgcaaaaa gaaaacggat aaatgccaac atagtcacat tccagctaaa agcccggcaa
    11941 gccgggcgtt aacacatcag aacttcaacg acccctgcat atacagcgtg cgcggctggc
    12001 ctgcgtagat gcctttgttg ttgtcgtcat aagagcggat gaagtagtcc tggtcgaaga
    12061 tgtttttcac accgaacgcc aggttcagat ctgccatctg cgggccaaag tcatacgcca
    12121 cgcgtgcgcc ccagagcatg aagccgggaa tgcggccggt actgccgtcg gcgctctctt
    12181 tcaccgtatt ggcgttatcc gcaaactggc tggactggaa atcgctgttc agattgaacg
    12241 tccagttgcc cggcttgtag tccacgccca gcgtgccttt atgtttcggg gagaatggca
    12301 cctgattgcc ataggtgtcg cctttctcgc ggatttccgc gttcacatac gcatagctgg
    12361 cgtagacgga aacgttatca agcgttggcg ttagcgtacc caggtcgtaa cgtgcctgcg
    12421 tttccagccc ggtatggcgc gttttgccac gtgcagtgac ggtgtcgttg gtctggttgg
    12481 agtcgtactg attgttaaag ttaatcagga acagccccat ttccgccgtc agcgcgccgt
    12541 catcgtagcg ggtaccgagt tcccaggttc gcgctttttc cggttccaca ttgccgcttt
    12601 gcacagcctt gccaatctgg ctgtactgta cggtgccgaa cgagccttca gtgtttgcat
    12661 aaagattcca gctatcagtc aggtgataga gcacgttcaa cgccggaagc ggtgcgttat
    12721 agctcacctc ttcgtgcgtg cctttgatgg cgttgttctg gtatgactcg atatgttcga
    12781 aacgcatacc cggcgtgatg gtccagttgc cgatgtcgat tttgtcatcc agataccagg
    12841 cgtgcgcctc ggtgccggaa cgcgtgtcgc ggtcgtaagg gcttgagccg gacggcaact
    12901 gcccgctgct ggtggcggtg tagtaacgca tttcatgcgt tgattcattc acatagcgat
    12961 agcccacgcc cacttcgtgc gcggaagggc cgatcataaa gctctggctg tagcgtggct
    13021 caataccgcg cacccagtag ttacgcggcg agagggtgat gcgtttgcct tgctccaggt
    13081 agccgctgcg cagggtttgg gtgtagaacc cctgaatgtt gaatttatgc tggctgtccg
    13141 gctggaactg gtagcccagg ctcgccagct tgcgacggcc ccagaagcgg tcatacgggc
    13201 gggtggattg ccagcgatcg gcgtcgtaat ccgcgcggga caggccgccg ggcatgtcgg
    13261 cttcaccatc gtaatattgc agcaggctgt tgaaggtgtg cacctcattc ggcgcatatt
    13321 tgcttttcag catcaggtcg tcgatgcggg tggcgctgtg ctcgcgccag tcactgccgc
    13381 gcgtgccgga gtagagcagc gcggtgccaa aaccgttgtc cgctgtgccg cccaccatca
    13441 ggttgtgcgt ctctttcggg ttgttttgtg aagaggttgg gctgagctga ccttccaccc
    13501 ccgcctcgat accaaagtcc tgcggaatgg cgcgggtaac aaagttcacc acgccgccca
    13561 cgctctgcgg tccgtaacgc accgcaccac caccgcgcac cacgtcaatg gcatccatgt
    13621 tgccgagcga aacgggagcc agtgaaagct gcggctgacc gtaaggggca aaggggacgg
    13681 ggatgccgtc catcaggacg gtcgagcggc tggcgaggcg tgggttcagg ccccggatgc
    13741 caaagttcat cgccaggtcg tggctgccgg tgccgttgtt ttccggcgcg ctgacgccag
    13801 ggatgcggtt aagcacctca cgcatggtgg ttgcgccggt tttggcgaaa tcctcacggc
    13861 ggatcacgtc acgcgcgcca gcatgttcaa atacgtcgtt ttcacgcgca tcgcccagcc
    13921 agtcgccgac cacggtcagg gcatcttctt ttggcgcggg cgcggtctcc agcgtccagc
    13981 tgttatttct cagcggtttt acctgcagtc cgctgccgtc cagcagttgt tgcaggccgc
    14041 tctcgacgtc gtaatctccg tgcaggccgt tgctctgctt gccgcgcgtc aggctggcgt
    14101 caaccgagag ggtaattccg ctgtgtgcgg catactgatt gagcgctttg tcgagcgatc
    14161 ccggtgcgat atcaacctgt gcagcaaaag cggaaaacga gagaccggcc agcggcagca
    14221 ggctcaggcg aatggcgtta accaaaggtg ttgttttacg aaaaacgcgt aacggcgtca
    14281 taccttctcc atcatcattt ttgttgtgtt cagctatgag tcgaacgaga agggtaaaaa
    14341 ggacaatcga aatgagaatt atttttctta cagcggtgaa atgtttatcc agtaccgcgt
    14401 aatagactga attttaacgg gaagcgtttg agcgataacg ttcaggatcg catcggtatt
    14461 tttcagcggg aacgtcccgc tcaggcgcag cccggcaacg gcgggatcgc agcgcagcac
    14521 gccgttgcgg taacggctta gcgtggctat cacctcaccc agcggtttat cgctgaagct
    14581 caggatgccc ttcgtccagc ttgtactctc gtcatccagc ggtttcactg cgccaaactc
    14641 agaggcgctg aactgcaggc tttcaccagc gttcacgatg cgtttttgcg cgggggcact
    14701 ggcgagaagc acttccacgg cgtgctgctg cacgtcaagc tgcgtgaaat tatcctgctg
    14761 gcggacggta aattctgtcc ctaaagcggt gagttggccc tgacgggtca ggacgcggaa
    14821 ggggcgttgc tgcgcatctt tcgcggtagt aatggcgatt tcaccgtacc agagccggac
    14881 ggtgcgctga tgcgcatcaa aacgcacatc cgccgcgctt tgggtattca gcgtgagcag
    14941 ggagccatct tccagttgct gacggctgac tgcgcctttg gcggtgcggt aatctgcccg
    15001 cagaccttcg ccggtttccg actgccagag ttgccagcct ccgccagcgc cgagcaacag
    15061 cagtaatcct ttcatcacgt gacggcgggt gaggcgggta tcgtgcagcg cccggctggc
    15121 aacgtcgcca ggcacaccgc caagctggtt gcgcaggttt tcaacctgct gccaggccca
    15181 ctggttatcc tgatcctgtt cataccactg ttgccagcgc gcttcctgtt gtgggctgac
    15241 gcgctcgccg cttagcacgg cataccagtg ggaagctgaa cgcagcgcct gacggcggga
    15301 atcggttaac gaaggattca taacccatgc tccagacgaa acagcaggca gtgctcgacg
    15361 gctttcgcca cgtatttttt cacggagctg acggaaacac cgagtttgtg cgcaatctcg
    15421 ctgtatgtca ggccatccag ttgcgaaagc agaaacgctt cacgtgtttt tccgtttagc
    15481 ccgtccagca tgctgtcgac gagttgtagg gtctcgagct ggctttcgcg ttcctcaggt
    15541 gaaggcgcta ccccctccgg cataagcgcc agcatctcca gatacgcttt ttccagcgcg
    15601 tttcggcgaa acaggtccac catcacgcgt ttggcgatag tgcacaggaa agagcgagga
    15661 tcgcggatcg tcgagagcgt ttcgctgctc attacccgca aaaaagtgtc ctgggcaatg
    15721 tcatctgcat caaaggcaga ctggagtttg cgcgtcagcc aacttttcaa ccagccgtga
    15781 tgggtgccat aaagcgactc gaacgttaag gaagctgtgg aagtggcacg gttagacatg
    15841 cggagtgcat caaaagttaa ttatcacgta gccatattaa tatgagaatg gtta
//
LOCUS       DAPHBJ010000097        15686 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.098, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000097 DAPHBJ010000000
VERSION     DAPHBJ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.098"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(32..2602)
                     /locus_tag="RHZ76_002738"
     CDS             complement(32..2602)
                     /locus_tag="RHZ76_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDV2813351.1"
                     /translation="MKFTLKDYQRDAVRDSLVNLRKARRYWHNENDKSAFSLTAVTGA
                     GKTVMAAAAFEALFHGDDEFNFDADPGAVVIWFSDDPALNEQTRFRLMEASDRINYND
                     MVVVEHPFSRRKFQPGKIYFLNTQKFGKNSLLVRGFDDKGGLFEAAPDAQAYTLWDTI
                     QNTIEDPDLTLYLVLDEAHRGMGSSTTSSEAAKSTIVQRLINGSAGVKGIPVVWGISA
                     TVERFDKAIVNAGHHVKLPNVVVDPAKVQESGLIKDDIILGVPDEAGDFDTVWVRRAA
                     DKLREASDAWAEYARQQETIREVVPLMVFQVPNMPDPNEVGRWLDSLFSRYPELPIDS
                     VAHVFGEHTTQRFGQHQVPYIEPQRVQESTWVRVLIAKDAISTGWDCPRAEVMVSFRA
                     ASDRTHITQLLGRMVRTPLARRIPGNERLNSVECLLPKFNTNTVRDVVNTLFKGDDTS
                     PPSGRILVNPVEMKPNPAASAAVWEVFEALPSQTRPQKGARPARRLTALAQELVDDGI
                     LDGAVRKAHAALHAVLNKFQTENAEKIKNKRNAVLVVDGKAVVASLKNKAMTFNEFWE
                     EADVAVIDDAYRRAARIFSPDVSRTYVEYLADKVADREEDAEEYLEAIMEARVTIAGM
                     GLVMEVQDYFDAEADKLAKAWLVEYTPQIKTLKDERKEAYRQIVEMSTEPQDVDLVRP
                     ENKFEMTRAREGEKETDLPVWKHHLLCDESGNYPAQLNHWETKVFEIETKREGFAFWY
                     RNPQYTGQSSLGIAYVEAEQYKIVRPDFLFFAEQDGKMVVDLVDPHGLHLADALPKLK
                     GLALYAEHHPGAYRRIESVAEVKGKLRVLDLKRQDVRNAIAVAENAETLFSSGLANDY
                     "
     gene            complement(2599..4725)
                     /locus_tag="RHZ76_002739"
     CDS             complement(2599..4725)
                     /locus_tag="RHZ76_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDV2813352.1"
                     /translation="MSRLTDLIAKAKAKDPQLAADLDREIKILSSRLPFGLNFERHSP
                     EVVELPLRPIRKGDKVRVLPERGSTKKGDQRLWQVKTIHKARKVADLELLDAADVETQ
                     TVALSNLVVIAEFRDTIWPGMVSTGKVQRGGDKPFHSVINGENYHVLKALTYTHRGKV
                     DAIYIDPPYNTGAKDWKYNNDYVEGDDIYRHSKWLAMMERRLLVAKELLNPSDSVLIV
                     SIDEKEYLRLGLLLEQLFPEARIQMVSSIIKPEGTNRPAEFSRTNEFLFFVMFSDARV
                     IPTNDDMSGRRAPATEVPIEWRNLRRRENSSVRAARPNQFYAVFVNEHTCSIHSVGEP
                     LPPDVDRQSVVPPTGCVAVFPLKPDGTEMLWGLTPGALRSLVSNGFARVANSGRGASS
                     MTIQYLAKGTITAIHSGDVTITGKDDQGAVQGFYTEKSSMPKTTWNKDSHNAQTSGTL
                     ILTSLLPGRRFPFPKSLYAVEDSLRFFVKNKPEAVILDFFSGSGTTAHAVMRLNKQDG
                     GRRQCISVTNNEVAADEQKKLREQGLRPGDLEWEKWGICDYITKPRVQAAITGKTPDG
                     EPIKGDYKFTDEFPMSEGFEENAEFFTLTYEAEKSVSHNLAYARIAPLLWLRAGARGK
                     RIDKLPADGWAVTDAYGLLTAVDQATPFIKAISHASDVRVAFIVTDDDRHFQSVTKRL
                     PKGVEPVRLYESYLTNFSFTSGELTE"
     gene            5044..5907
                     /locus_tag="RHZ76_002740"
     CDS             5044..5907
                     /locus_tag="RHZ76_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDV2813353.1"
                     /translation="MSKQEGINTINKAMAALPTRLSDTILKHIHSLTEYEPVIGIMGK
                     TGSGKSSLCNALFAGEVSPVSDVTACTREPLRFRLKVGERYMTIVDLPGVGENSARDT
                     EYAALYREQLPRLDLVLWLIKADDRALAVDEHFYHQVIGEVYRHKVMFVISQSDKAEP
                     TSVNGQLSMAQKQNVSRKICLLHELFQPVHPVCAVSVRLQWGLQVMAERIIKCLPREA
                     TSPVVSQLQHPFRTAAVQEKARDDFGETVGAVLDTVSAFPLIPAPVRAVIQAVRNTVV
                     SVARTVWDFFF"
     gene            5998..6819
                     /locus_tag="RHZ76_002741"
     CDS             5998..6819
                     /locus_tag="RHZ76_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDV2813354.1"
                     /translation="MTRLASRFGAANLIRRDRPLTREELFRVVPSVFSEDKHESRSER
                     YTYIPTISLLDSLQREGFQPFFACQTRVRDPGRREHTKHMLRLRREGQITGKQVPEII
                     LLNSHDGSSSYQMLPGLFRAVCQNGLVCGESFGEVRVPHKGDVVSQVIEGAYEVLGIF
                     DRVEEKRDAMQSLLLPPPAQQALAKAALTYRFGEDHQPVTESQILSSRRWQDESNDLW
                     TIYQRIQENLIKGGLSGRNAKGGRTHTRAVRGIDGDVKLNRALWVMAETLLTQLQ"
     gene            7036..7737
                     /locus_tag="RHZ76_002742"
     CDS             7036..7737
                     /locus_tag="RHZ76_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDV2813355.1"
                     /translation="MTQAERRHDRLAVRLSLIISRLVAGETLSVRKLAAEFGVLVRTL
                     RRDFRERLMYLDLEYQSGYCRLRTAGSEMQMVPDVLIFAHRSGLAGLFPGLDRRLVNA
                     LLMCDESPCVIAPANPVPSPSGALSFWRLIQAITGRRRVTLIAEGRRCERLAPCRLLI
                     HQQTWYLVAEYEGHIAVFTLDEIHLIQPLQETFRRNDSLCRLTEDPVFIQALPHFRFI
                     QQSLLTFVPADSPPE"
     gene            7778..8014
                     /locus_tag="RHZ76_002743"
     CDS             7778..8014
                     /locus_tag="RHZ76_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813356.1"
                     /translation="MPVIAIIAIVIIVIILNKTGVSDSLTALTLATVAALLTGGGAAG
                     AASVALTPFVGVPVGIFVGIYVFTKVVRLISGKK"
     gene            8014..8454
                     /locus_tag="RHZ76_002744"
     CDS             8014..8454
                     /locus_tag="RHZ76_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813357.1"
                     /translation="MKRKTLPLLALVASTLFLSACDDRSDDLKAISKFKDLTPPRFSD
                     VVSRQDDVREEWSQIVLSGPTLQVLRTRQSPDGCEGGSYYYLVDMEEKTVQPLMNALC
                     IADNISLQYHEKTDPRTRERFLEYTHDGKLMGRLLIPSNPDNRE"
     gene            8478..8945
                     /locus_tag="RHZ76_002745"
     CDS             8478..8945
                     /locus_tag="RHZ76_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813358.1"
                     /translation="MKINSLSRLMLAGVLLISFNASAVSGLWQQGYGQGNAEYSVTDG
                     SGKMFTINCTENPDQNGIYQHSVFLTLTGDKTLSSHDDSTDITVVMDNKQYAIPSTLG
                     WRNGDNAWFSFIMDIRKARQFDVYVNDRKAGTFTADLKNVEKVLPTPGDCGND"
     gene            9022..9261
                     /locus_tag="RHZ76_002746"
     CDS             9022..9261
                     /locus_tag="RHZ76_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HDV2813359.1"
                     /translation="MSQSVLLSPGPFSRIQAEAVTVAFRNITIEDDQGNHFRLVVRDP
                     EGLMVWRAWSFEPDGGAGLNRYIRQYGIIKEPSAL"
     gene            9361..9810
                     /locus_tag="RHZ76_002747"
     CDS             9361..9810
                     /locus_tag="RHZ76_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDV2813360.1"
                     /translation="MTQHTLILANEPQLIITATAVPNEQRIGFWPQHFGTIPQWITLE
                     PCIFTWMERFCDEYSGGIWSFYTLSNGGAFMTPDAEGDDKWHLFNGMNGNDAEMSAEA
                     AGIAVCLIEYSHHACRTECDAMTEHYYRLRDYALQHPESSAILRIID"
     gene            9819..10301
                     /locus_tag="RHZ76_002748"
     CDS             9819..10301
                     /locus_tag="RHZ76_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HDV2813361.1"
                     /translation="MMEQSLIPLTPALPLTAQRTVKRALTLLDRHLRETGVAFTSTQA
                     ASDWLKLKMAGLEREEFMVMYLNQQNQLIAYETLFAGSISSTEVHPREVVKRALYFNA
                     AAVILAHNHPSGNIIPSQADKTITQRLVKALHLVDIRVPDHLIVGGTQILSFAEHGLL
                     "
     gene            10310..10531
                     /locus_tag="RHZ76_002749"
     CDS             10310..10531
                     /locus_tag="RHZ76_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDV2813362.1"
                     /translation="MKITSKRRAMTIYRQHPASRIFRFCTGRYQWHGSVCHYTVKVVP
                     DIFGVLAVYAERHQDRNGPYTNLMSITLS"
     gene            10549..10866
                     /locus_tag="RHZ76_002750"
     CDS             10549..10866
                     /locus_tag="RHZ76_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDV2813363.1"
                     /translation="MSNTTWGLQRDITPRLGARLVQEGNRLHYLADRASITGKFSDAE
                     CPKLDVVFPDFISQIESMLTTGELNPRHAHCVTLYHNGFTCEADTLGSCGYVYIVVYS
                     TQR"
     gene            10887..11216
                     /locus_tag="RHZ76_002751"
     CDS             10887..11216
                     /locus_tag="RHZ76_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDV2813364.1"
                     /translation="MNTLPATTPQAAKLYLSPVSVWQMLLTYLLEQHYGLTLNDTPFS
                     DETVIQEHIDAGVSLSDAVNFLVEKYGLIRIDRKGFSWQEQTPYISVVDILRARRSTG
                     LLKTNVK"
     gene            complement(11580..>15686)
                     /gene="ypjA"
                     /locus_tag="RHZ76_002752"
     CDS             complement(11580..>15686)
                     /gene="ypjA"
                     /locus_tag="RHZ76_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HDV2813365.1"
                     /translation="SQIVKVQGHADGTTINKSGSQDVVQGSLATNTTINGGRQYVEQS
                     TVETTTIKNGGEQRVYESRALDTTIEGGTQSLNSKSTAKNTHIYSGGTQIVDNTSTSD
                     VIEVYSGGVLDVSGGTATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLL
                     ENGGHLDINAYGSANKTIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGG
                     TQNINNYGIATGTNINSGTQNIKSGGKADTTIISSGSRQVVEKDGTAIGSNISAGGSL
                     IVYTGGIAHGVNQETGSALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTV
                     VAKTSAKNTTIDTGGKLIVQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIAS
                     TTSGTLIEGTNSYGDAFYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYG
                     KDVGTVLNSAGTQTIYASATSDKANIKGGKQTVYGLATEANIESGEQIVDGGSTEKTH
                     INGGTQTVQNYGKAINTDIVSGLQQIMANGTAEGSIINGGSQVVNEGGLAENSVLNDG
                     GTLDVREKGSATGIQQSSQGALVATTRATRVTGTRADGVAFSIEQGAANNILLANGGV
                     LTVESDTSSDKTQVNMGGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVS
                     ANGEAIKTKINEGGTLTVNDNGKATDIVQNSGAALQTSTANGIEISGTHQYGTFSISG
                     NLATNMLLENGGNLLVLAGTEARDSTVGKGGAMQNLGQDSATKVNSGGQYTLGRSKDE
                     FQALARAEDLQVAGGTAIVYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAVRITD
                     SATLTLGNGVDTTLADLTAASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGDVYLSAQ
                     TAAPATTNGIYNTLTTNELSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGV
                     SPQSDDAMTLVKTGGGDASFTLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDP
                     IPNPKPDPKPDPKPDPNPKPDPTPDPTPTPVPEKRITPSTAAVLNMAATLPLVFDAEL
                     NSIRERLNIMKASPHNNNVWGATYNTRNNVTTDAGAGFEQTLTGMTVGIDSRNDIPEG
                     ITTLGAFMGYSHSHIGFDRGGHGSVGSYSLGGYASWEHESGFYLDGVVKLNRFKSNVA
                     GKMSSGGAANGSYHSNGLGGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMK
                     SKSVDTRSIYRELGATLSYNMRLGNGMEVEPWLKAAVRKEFVDDNRVKVNSDGNFVNY
                     LSGRRGIYQAGIKASFSSTLSGHLGVGYSHSAGVESPWNAVAGVNWSF"
BASE COUNT         3642 a         4193 c         3780 g         4071 t
ORIGIN      
        1 ggccatttta tattcaattt atcttaggat cttaatagtc attggcaagc ccactactga
       61 ataacgtttc cgcattttca gctacagcta tagcatttcg tacatcctgc cgtttcaaat
      121 ctaacacccg taatttaccc tttacttcgg ctacggattc gattcgtctg taagcaccag
      181 gatgatgttc ggcatatagc gccagtcctt tcagtttggg caaagcatca gccagatgta
      241 ggccatgtgg atctaccaag tccacaacca ttttgccatc ctgttcggca aagaacagga
      301 aatcgggacg aacaatcttg tactgttcgg cttcaacata ggcgattccc agtgatgact
      361 gccctgtgta ctgtggatta cgataccaga aagcaaatcc ttcacgtttg gtttcgatct
      421 caaagacctt ggtttcccaa tggttcaact gagccggata gttcccgctt tcgtcacaca
      481 acaaatggtg tttccagact ggaaggtcag tttccttttc accttcacgc gccctagtca
      541 tttcaaactt gttctccggc ctgaccagat ccacatcctg cggttcggta ctcatttcga
      601 cgatttgacg gtaagcctct ttacgctcat ctttcaacgt cttgatctgc ggtgtgtact
      661 cgaccagcca cgccttcgcc agtttatcgg cctcggcatc gaagtaatcc tgtacctcca
      721 tcaccagccc catgccagca atggtcacac gagcttccat gattgcttcc agatattcct
      781 ctgcatcctc ttctcgatcc gcaaccttat cagccagata ttcgacatag gtacgtgaaa
      841 catccgggct gaagattcgt gctgcacgcc ggtaggcgtc atcaatcacc gctacgtccg
      901 cttcttccca gaattcgttg aaggtcatag ctttgttctt caggctggca accactgcct
      961 taccatccac aacgagcact gcattgcgct tgtttttgat tttttcagca ttttctgtct
     1021 ggaacttgtt caagacagcg tgcaaagcag catgggcttt cctgactgcc ccatcaagaa
     1081 tgccgtcatc aaccagttcc tgtgccagtg cagtcagacg tctggctggt ctggctcctt
     1141 tctgtggtcg ggtttgtgaa ggtagcgctt caaatacttc ccatactgca gctgaagctg
     1201 ccgggttagg tttcatctcc acagggttaa ccaagatacg cccgctcgga ggagatgtat
     1261 catcgccttt aaacaaggta ttgacgacat ctctcacggt attggtatta aacttgggta
     1321 ataggcattc gacagagttt aagcgctcat tacccggaat gcgtctagcc agtggagtac
     1381 gtaccatacg ccccagtaat tgcgtaatat gcgttctgtc gctggcagcg cggaaggaaa
     1441 ccatcacttc ggctcgcggg caatcccagc cagtgctgat ggcatcttta gcaatcagta
     1501 cgcgcaccca agttgattcc tgcacccgct gcggttcaat ataaggcact tggtgttggc
     1561 cgaaacgttg tgtggtgtgt tcaccgaaga catgagcaac actgtcaatg ggcaattcag
     1621 gataacggga gaataacgaa tccagccagc gtccgacctc gttcggatct ggcatattgg
     1681 gaacctggaa taccatcaga ggcactactt cgcgtatggt ttcctgctgc cttgcgtatt
     1741 ctgcccaggc atcgctcgct tccctgagtt tgtcggcagc acggcgtacc cacactgtat
     1801 cgaagtcacc agcttcatca ggcacaccca gaatgatatc gtccttaata agtcccgatt
     1861 cctgcacttt agcggggtca accaccacat tcggcagttt gacatgatga ccagcattga
     1921 caatggcttt gtcgaaacgt tcaactgtgg cggaaatacc ccataccaca ggaataccct
     1981 tcaccccagc cgaaccgttg atgagccgtt gcacaatggt acttttagct gcttcgctgg
     2041 aagtcgttga cgaccccatc cccctgtgcg cttcatctag cactaagtaa agggtcagat
     2101 ctgggtcttc gatggtgttt tgaatagtat cccacagcgt ataagcttgt gcatcagggg
     2161 ctgcctcgaa aagcccgcct ttatcatcaa agccgcgcac caacagacta tttttgccaa
     2221 acttctgcgt gttaaggaaa tagattttcc caggctgaaa cttgcgccga ctgaaagggt
     2281 gttcaacgac taccatatcg ttatagttga tacggtcact ggcctccatc agacgaaaac
     2341 gggtttgctc gttcagtgca ggatcgtcac tgaaccagat cactaccgcg ccgggatcgg
     2401 catcgaaatt aaattcgtcg tcgccatgaa ataatgcttc aaacgcagcg gcagccatga
     2461 cggtcttacc cgcaccggtc acagccgtta aagagaaggc gctcttgtca ttttcgttat
     2521 gccagtaacg gcgagccttt ctgagattca ccagcgaatc acgcaccgca tcgcgctggt
     2581 agtccttgag agtgaacttc attctgttaa ttccccactg gtgaagctga aattggtcag
     2641 gtacgattcg tataaacgta ctggctcaac accttttggc agacgcttgg tgacagactg
     2701 gaaatgacga tcatcgtcgg tgacgataaa ggccacccgt acgtcactag catggctaat
     2761 ggctttaatg aaaggtgtgg cttgatccac cgctgtcagc aggccataag catcagtcac
     2821 cgcccatcca tcagcaggca gtttgtcaat ccgtttgcca cgagcccctg ctcgcaacca
     2881 cagcaagggc gcaatacgcg cataagccag gttatggcta accgacttct ctgcttcata
     2941 agtcaaggtg aaaaattcgg cgttttcctc aaagccttca gacatcggga attcatcggt
     3001 aaacttgtaa tcgcctttaa tcggttcacc atcaggggtc ttgcctgtga tggcggcttg
     3061 cacacgcggc tttgtaatat agtcgcagat gccccatttt tcccattcga gatcgccggg
     3121 gcgtaagcct tgttcgcgca attttttctg ttcatcggca gcaacttcgt tgttagtgac
     3181 ggagatacat tggcgacgcc caccgtcctg cttattcaag cgcattacgg catgggcggt
     3241 tgtaccggag ccggaaaaga agtcaagaat gacagcctct ggtttgtttt tcacgaagaa
     3301 gcgaagggaa tcttcgacgg catatagtga tttggggaag gggaatcgtc tacccggtaa
     3361 gagagaggta aggatgagtg tccccgaagt ctgagcgttg tgggagtcct tgttccaagt
     3421 tgtcttgggc atcgatgatt tctcagtgta gaagccctga acagcgcctt gatcatcttt
     3481 tcctgtaatt gtaacgtcac ctgaatgaat ggctgtaatg gtccctttgg cgaggtactg
     3541 aattgtcatg ctcgaagcgc cccgaccgga gtttgccact ctagcgaagc cattagaaac
     3601 taagctccgt agcgcgcctg gtgttagccc ccaaagcatc tccgttccat caggcttgag
     3661 cggaaacaca gcgacacaac cagttggtgg caccacactc tgacgatcaa cgtctggagg
     3721 gagtggctcg ccgacagagt ggatactaca agtgtgttcg ttaacaaaca ctgcgtagaa
     3781 ttgatttggt cgcgcagcac ggacgctaga gttctctcta cgtctgagat ttcgccactc
     3841 aattggaact tctgttgccg gagctcggcg tccagacatg tcgtcgttag ttggtattac
     3901 cctcgcgtca gagaacatca caaaaaacaa gaactcgttt gtacgcgaga actctgcagg
     3961 ccggtttgtt ccttccggct tgatgattga tgaaaccatt tggatacgag cttctgggaa
     4021 taactgctcc aacagcaatc ccagccgtag atattccttc tcgtcaatac tgacgatcag
     4081 caccgaatct gacgggttca acaactcctt cgctaccagc aagcgacgtt ccatcatcgc
     4141 cagccatttg ctgtgtcgat agatgtcgtc accttcgacg taatcgttat tgtatttcca
     4201 gtccttcgcc cccgtgttat aaggcgggtc gatatagatg gcgtcgacct tgcctctatg
     4261 cgtgtaggtc agcgctttca atacatggta gttctcgcca ttgatgactg agtggaaagg
     4321 cttgtcaccg ccgcgctgta cttttcccgt actgaccata cccggccaga tcgtgtcacg
     4381 aaattcagct ataaccacaa ggttactcag cgctacagtc tgagtctcga catcagccgc
     4441 gtccagcaac tcgagatcag cgaccttcct cgccttatgg atagttttaa cctgccacaa
     4501 ccgttgatca cctttcttag tggacccacg ctctggcaga acgcggactt tgtctccttt
     4561 gcgaataggc cgcaatggca actcaacaac ttcagggctg tgtcgttcaa aattaagccc
     4621 aaaggggagg cgtgacgata ggatcttgat ttctctatca agatcagcag ccagttgcgg
     4681 atctttggcc ttcgctttag ctattaaatc agtcagcctg gacatatatt ccttttttct
     4741 tatcaattct gagcatttta ggttcaagcg ttcggctatc aggaaagccc aatacgttga
     4801 ttcatagaag gaatcagaac gctaaaaaat tggcggtaat attaacacta atcaacgact
     4861 tcatcgatcc ttaaaccgag agatataggt tagtcccatc atgtctgtag cgcagtttcc
     4921 tctatccaag tggcaccaga taatccaagt attttcagac atatatcatc tctctgaaca
     4981 caaccatcca tacggcgaac ctgtggttcg ccgttgttac tttattttct gtcaggaaaa
     5041 atcatgagta aacaagaggg tatcaatacc atcaacaagg cgatggccgc gctgcctact
     5101 cgactgtctg atacgatcct aaaacacata cactcgctta ctgaatatga acctgtgatc
     5161 ggtattatgg ggaaaacagg atcaggaaaa tccagcttgt gtaacgcctt atttgccgga
     5221 gaagtctcac cagttagtga tgttactgcc tgtacccgag aaccactacg ctttcgtttg
     5281 aaggtcggtg agcgttatat gacgatcgtt gaccttccag gtgtgggaga aaacagtgcc
     5341 cgcgataccg agtatgccgc gctgtaccgt gaacaacttc cccgactcga cctagtattg
     5401 tggctgatta aggctgatga ccgcgcactg gcagtggatg agcactttta tcatcaggtt
     5461 attggtgagg tataccggca taaggtgatg tttgttatca gccagtcgga caaggccgag
     5521 cccaccagcg ttaatggtca attgtccatg gcgcagaagc agaatgtcag ccgcaaaatc
     5581 tgcctgctgc atgaactgtt ccagcccgta catccggtgt gtgccgtgtc ggtccgtctg
     5641 cagtgggggc tgcaggtgat ggccgagcga ataattaagt gtctgccacg cgaagccacc
     5701 agcccagtgg tgtcgcaact gcagcatccc tttcgcacag cagcagtcca ggagaaagcg
     5761 cgtgacgatt ttggtgaaac ggtcggcgcg gtactggaca cggtaagtgc tttcccactc
     5821 ataccggctc cggtacgtgc cgtaatacag gccgtccgta acaccgtggt atccgttgcc
     5881 cgtactgtgt gggatttctt cttctaagta tttaacccat ccctgttatt tccctaaccc
     5941 tgtctcctgt gcgacgggga tttttttatt tgtttttcca ttatgaggag tctgcttatg
     6001 acccgtctgg cctcgcgctt tggtgcagca aatcttatcc gccgcgaccg tccgttaacc
     6061 cgtgaagagt tgtttcgagt ggtccccagc gtattcagcg aggacaaaca cgaatcccgc
     6121 agcgaacgtt atacctatat tccaaccatc tccctgctgg acagcctgca gcgggaaggc
     6181 ttccagccat tctttgcctg tcagacccgt gttcgcgatc ctggccgtcg tgaacatacg
     6241 aagcacatgc tgcgcctgcg tcgggagggg cagattaccg gtaagcaggt gccggaaatt
     6301 atcctgctca actcccatga tggctcaagc tcttaccaga tgttgcctgg cctgttcagg
     6361 gctgtgtgtc aaaacggcct cgtctgcggt gagtcgtttg gcgaggtacg ggtgccgcac
     6421 aagggggatg tggtgagtca ggttattgag ggggcgtatg aagtactggg gatttttgac
     6481 cgtgtggagg aaaagcggga tgccatgcag tcattgctgt taccaccacc tgcgcagcag
     6541 gcactggcaa aagccgcgct aacttatcgc tttggtgagg accaccagcc ggttactgaa
     6601 tcgcagatac tctcctcgcg ccgctggcag gatgaaagca atgacctgtg gaccatctat
     6661 cagcggattc aggaaaacct gattaagggc ggactcagcg ggcgtaatgc taaaggcgga
     6721 cgaacgcata cccgtgccgt acgtggcatt gacggggacg tgaagcttaa ccgtgctctg
     6781 tgggtgatgg cagaaacact gctcacgcaa ctgcagtaga cgtcttatgt tgccacgttg
     6841 ttaatatcgg acaccacctg tccgcatcgc tatgtgctcg tgtgcctcaa tctcccggtt
     6901 atagcctgta acccccgttg catctggctt ttgcagaaat aaaaaatagt ttctgcgttg
     6961 tccataccct gtccgccccc ctctttaaag taatcacatc attttcagcc agttaacttt
     7021 cctggagaac ctctaatgac acaggcagag cgccgccatg accggctggc tgtcaggctg
     7081 tcactgataa tcagccgtct ggtggcgggt gaaacgttga gtgtgcgtaa actggccgct
     7141 gagtttggtg tgttagtgcg cacgctgcgg cgtgattttc gtgaacgact gatgtatctg
     7201 gacttggagt atcagtccgg atactgccgc ttacgcactg ctggcagtga aatgcagatg
     7261 gtgcccgacg tgcttatctt cgcccaccgc agcgggctgg ccgggctttt tcccggcctt
     7321 gaccgccgtc tggtgaatgc tctgctgatg tgcgatgagt ctccctgcgt gatagcacca
     7381 gccaatccgg ttccttcgcc ttcaggagca ttgtctttct ggagactgat tcaggccatt
     7441 accgggcgca ggcgtgtgac gctgattgcg gaggggcgac gctgtgagcg cctggctccc
     7501 tgccggttac tcatccacca gcagacctgg tatctggtgg ctgaatatga agggcatatc
     7561 gccgtattca cacttgatga aatccatctg attcagcctt tgcaggagac ttttcgccgc
     7621 aatgacagtc tgtgccgtct gactgaagac ccggttttca ttcaggcttt accccatttt
     7681 cgctttatcc agcagtcact gcttacgttt gttccggctg acagcccacc ggaatagcgc
     7741 aggcgttgtt atcaacccgg caacagggag gagcccagtg cccgttattg ccattatcgc
     7801 cattgttatc atcgtcatca ttctgaataa aaccggggtt tccgacagcc ttacggccct
     7861 gacacttgcc accgttgccg cactgctgac gggaggtggc gcagccggtg ctgccagtgt
     7921 tgcgctgacg ccgttcgtcg gcgtgccggt gggtattttc gtggggattt atgtctttac
     7981 caaagtggtt cgtctgattt caggaaaaaa ataatgaaac gtaaaacact acctctactg
     8041 gcgctggttg cctccactct gtttctgagc gcctgcgatg acaggagtga tgacctgaaa
     8101 gctatcagta aatttaagga cctcacgcct ccgcgcttca gcgatgtggt gagccgtcag
     8161 gatgatgtca gggaagagtg gtcgcagatt gttctttctg gccccactct gcaggtctta
     8221 cgcacacgcc agtcgcccga tggttgcgag ggtggcagtt actactatct ggtggatatg
     8281 gaggagaaaa ccgtccagcc gctgatgaat gcgctgtgta ttgccgacaa catcagcctg
     8341 caataccacg agaaaactga cccgcgcacc cgggagcgtt tccttgaata tacccatgac
     8401 ggcaaactga tgggacggct gctgataccc tcaaatcctg ataaccggga ataaatacga
     8461 agacaaagga gacagaaatg aaaataaatt ctttaagtcg gctcatgctc gcaggcgtgc
     8521 tgcttatcag cttcaatgcc tctgcggttt cggggctctg gcagcagggg tacggtcagg
     8581 gcaatgcgga atacagtgtg acagatggca gcggaaagat gtttaccatc aactgcacgg
     8641 aaaatccgga ccagaacggt atttaccagc actcggtttt tctgaccctc acaggtgata
     8701 aaacgctcag ctcgcatgat gacagtactg acatcacagt ggtgatggac aataagcaat
     8761 atgccattcc ctcaaccctt ggctggcgta acggggataa tgcctggttt agtttcatca
     8821 tggatattcg taaggccagg cagttcgacg tctacgtcaa tgatcgcaaa gcggggactt
     8881 tcactgctga tctgaagaac gttgagaaag tcctgcccac tcccggagac tgcggtaacg
     8941 actgacagcc gcatcttccc ccgcaaacta accccgacag ccaccagtct gtcggggttt
     9001 tcttttatca ggagcctgca aatgtcccaa tcagtgttgc tgtcaccggg accgttctcc
     9061 cggatacaag cggaagcggt cacggtagcg ttccgcaata tcaccattga agacgaccag
     9121 ggtaatcact tccgactggt ggttcgtgac ccagaaggcc tgatggtctg gcgggcatgg
     9181 agctttgaac ctgacggcgg agctgggctt aaccgctata tccggcagta cggcattata
     9241 aaggagccct cagcactcta attgaacgag cacttaacgc ggcatcacac ttcataccac
     9301 atcatttatt ccccataacg acagcctgag ttgctggcga ttttattcct ggagaacacc
     9361 atgacacagc acaccctcat cctggctaac gagccacagt tgattatcac cgccacagct
     9421 gtacccaatg aacaacgtat cggcttctgg ccacaacact ttggcactat cccgcagtgg
     9481 ataacgctgg aaccctgtat tttcacctgg atggagcgct tttgtgatga gtacagcggt
     9541 ggtatctggt ccttttatac gctcagcaat ggcggcgcgt ttatgactcc tgatgctgaa
     9601 ggtgacgata aatggcatct gttcaacggc atgaacggca atgatgcgga aatgagcgcg
     9661 gaagccgcag gtatcgctgt ctgcctgatt gaatacagcc atcacgcctg tcgcaccgaa
     9721 tgcgatgcca tgacggagca ctattaccgc ctacgggatt acgccctgca gcaccctgaa
     9781 tccagcgcca ttctgcgaat cattgattaa ggatacttat gatggaacag tcacttatcc
     9841 cactgactcc ggcactacca ctaaccgcac aacgcacggt aaaacgcgcc ttaacgctgc
     9901 ttgaccgaca cctgcgcgaa acaggcgtgg cattcacctc cacccaggct gccagtgact
     9961 ggctgaagct gaaaatggcg ggactggagc gtgaagaatt tatggtgatg tatctgaatc
    10021 agcagaacca attgattgcc tacgaaaccc tgtttgccgg ttccatcagc agcaccgagg
    10081 tacatccccg tgaggtggtc aaacgcgccc tgtacttcaa tgcggcagca gtgatactgg
    10141 cacataacca tccctccggt aacatcatac ccagccaggc agataagacc atcacgcagc
    10201 gtctggtgaa ggcgcttcat ctcgttgata tccgtgtgcc tgaccatctc attgtcggtg
    10261 gcacgcagat attgtcgttc gctgaacacg gtttgctttg aggtatgaca tgaaaattac
    10321 cagtaaacgc cgggcgatga cgatttaccg tcagcatccg gcctcccgca tttttcgatt
    10381 ctgcactggc agataccagt ggcatggcag tgtctgtcat tacaccgtta aggtggttcc
    10441 tgacattttc ggtgtgctgg cggtatacgc cgaacggcac caggatcgca acggacctta
    10501 taccaatctg atgagtatca ccctgagctg acaataaaga ggttatagat gagcaacacc
    10561 acatggggcc tgcagcggga tatcacgccg cgcctgggag cacgtctggt gcaggagggc
    10621 aaccggctgc actatctggc tgaccgggcc agtatcaccg gtaagtttag tgacgccgaa
    10681 tgtcctaagc tggatgtggt atttccagat tttatcagcc agatagagtc gatgttgacc
    10741 actggtgaac tgaatccccg tcatgcccac tgcgtcaccc tgtatcacaa cggtttcacc
    10801 tgcgaagccg atacccttgg cagttgcggc tacgtataca tcgtcgtcta ttccactcaa
    10861 cgctgactaa tttcacgaga gcaaacatga atactctacc tgcaacaact ccgcaggcgg
    10921 cgaagctata tctgtcgccc gtgtctgtct ggcaaatgtt actgacatac ctgctggaac
    10981 agcactatgg cctgaccctg aacgacacac cattcagtga tgaaaccgta atacaggaac
    11041 acattgatgc tggagtatcg ctcagcgatg cggtgaactt tctggtagaa aaatacgggc
    11101 ttatccgtat cgaccgaaag ggattttcgt ggcaagaaca aaccccgtat atttccgtag
    11161 tggatattct gcgagcaagg cgctctaccg gcttgctaaa aactaatgtg aaataaacgc
    11221 ttaaatacaa agcagactaa aggaaagcaa aatgctaatc tcacaaacga agaggcttct
    11281 actgtaacct cccctcccct gcaaaaacct gacattttct tttaggacca acaatgggac
    11341 caaaatgaaa attgaactga aggttatcag ctattaaaca acaatttaca caaccaattc
    11401 agactccgcc agcccaccaa tcatgattgg acggtgtaag gacaacacca acaaaaacag
    11461 gaagttagaa gtctcagcaa aacaccgacc agacggtgag gagacataaa aggatacgca
    11521 aaggagccgc ggctcctggt aacatgaaag cccacagatg tgggcttttt cgttgatggt
    11581 cagaacgacc agttcacacc agctaccgcg ttccacgggg attccacacc ggcactatgg
    11641 ctatacccca ccccaagatg cccgcttaac gtactgctga atgaggcttt aatacctgcc
    11701 tggtatattc cacgtctgcc cgacaaataa ttgacgaaat taccgtcact attcactttc
    11761 acccggttat catcgacaaa ttctttacgc acagccgcct tcagccacgg ctcaacttcc
    11821 ataccgttcc ccagacgcat gttgtaactc agcgttgcgc ccagttcacg atatatactg
    11881 cgggtatcga ctgatttcga tttcatgcca ttggataaat gatattcggg gttatcagcg
    11941 gtgaaccccg ttaacgatgc atacggcgtc aggttccagt taccatcggt aaatcgcatc
    12001 ccggtttcaa tgtgaccgcc cagcccgttg ctgtggtaac tgccattggc ggctccaccg
    12061 ctgctcattt tacctgctac gttactttta aaacggttca gcttcacgac accgtccaga
    12121 tagaaaccac tttcatgttc ccaactggca tagccgccca gagaataact gcccacactg
    12181 ccatgtcctc cgcgatcaaa accgatatgt gaatgggaat agcccataaa agcgcctagc
    12241 gtggtaattc cttcaggaat atcattacgg ctgtcgatcc ccactgtcat tccggtcagc
    12301 gtctgctcaa acccggcacc cgcatcggtg gtgacattat tacgggtgtt atacgtcgcc
    12361 ccccagacat tattgttgtg tggactcgct ttcattatgt tcaaccgctc gcgaatactg
    12421 tttagctcag catcaaatac caacggtaat gttgctgcca tattgagtac ggctgccgta
    12481 gaaggcgtaa tgcgtttctc cggaacgggt gtcggcgttg gatcgggagt agggtctggt
    12541 tttggatttg ggtctggctt tggatcgggt tttgggtctg gctttggatt tgggatgggg
    12601 tccgggttgg gtttgacatc attggtcagg ttccagttgc tgttgccgtc acttttcagg
    12661 acatactcat aggtcccaag atcaacgaaa ccgccggtat tgcccagcgt aaacgaagca
    12721 tcccctcccc ctgttttcac cagcgtcatc gcgtcgtcag actgtgggct gacgccggta
    12781 tcctgaacaa agattttaaa attaccagtg gcgttgttgt tgacgaccag ttgatcgccc
    12841 cgggagcctg caacgttggt atgcaggtag aaattaccgc taccggaaag ttcattggtt
    12901 gtcagcgtat tgtagatacc gttagttgtg gcaggcgctg ctgtttgtgc tgacaaataa
    12961 acatcaccgt cgttgagtag caaactgttt actctatatt cgcagttgct ggtacctgca
    13021 caggaattat tgctgttaag ccagacactg ccccggctgg cagccgtcag gtcggcaagc
    13081 gtggtatcga cgccatttcc cagagtcaat gtcgcgctat cggtaatccg gaccgcccct
    13141 tcgagtttaa ctggcgtaac attatcccgt ggcgtcatta acgacaggct tcctgtcgcg
    13201 ccactgaccg atgcatccgc cagcgtacct gcgtagacga ttgccgtacc gccagcgacc
    13261 tggagatctt ctgcccgggc cagagcctga aactcatctt ttgaccgccc aagggtatat
    13321 tgcccgccag agttaacctt tgtggcggag tcctgaccca ggttttgcat tgccccaccc
    13381 ttgccaaccg tggagtcgcg agcttcggta cctgctaata ccaatagatt accgccattt
    13441 tccagcaaca tattggtcgc taaattgccg gaaatggaaa aagtaccgta ctggtgagta
    13501 ccgctgattt caataccgtt agccgtgctc gtctggagag cggcaccgct gttctggacg
    13561 atatctgtcg ctttgccatt atcgttgact gtcagcgtac cgccttcatt gatctttgtt
    13621 tttattgcct ctccgttagc tgaaactgtt tgtattccgc cgtcgttaat tgttgtctca
    13681 ttcgccacac cctcgacaat ttgttcaccg ccggtgagcg tcgtgcctgt cgcagtggct
    13741 tttgttttga cgatctcccg tccgcccata ttgacctgtg ttttgtcaga agaggtgtct
    13801 gactccacgg ttaacacgcc gccatttgcc agcaggatat tgttcgccgc accctgctcg
    13861 atgctgaacg cgacgccatc cgcgcgtgtt cctgtgaccc gcgtcgccct ggtggttgca
    13921 accaaagcgc cctggctact ctgctgtatc cccgttgcgc tgcctttctc ccgcacatcg
    13981 agtgtgccgc cgtcattaag caccgagttt tcagccagac cgccctcatt aactacctgt
    14041 gaacccccat taataatgga accttccgct gtcccgtttg ccataatttg ttgtaggcca
    14101 gagacgatat cggtattgat tgccttacca taattctgaa cggtttgcgt gccaccattg
    14161 atgtgtgttt tctctgttga cccaccatca acaatttgtt caccactttc gatatttgct
    14221 tcagtggcta aaccatatac cgtttgcttg ccacctttga tatttgcttt atcagaagtg
    14281 gcactggcat atattgtttg ggtgccagca ctattgagta cagtgccaac atcttttcca
    14341 taaacatcca ttttgccgtt ggcattaata atcgtgtcaa ctgcccggga accagtgacg
    14401 actgttaatg agccagcgtt ttccagcact acatttttag cttctgaatt cctgatgtag
    14461 aaagcatcac cataactgtt ggttccttcg ataagtgttc cggaggtcgt ggaagcaatt
    14521 aatgcgccgc cggattgttg ctcaacatgc ttagcctcac caccgtcctg aacctccaga
    14581 acgccgccat tattaagtct ggtgctatct gttttagcct ccttctggac aatcagctta
    14641 ccgccagtat caatggtagt atttttcgcc gaggttttag ccactaccgt cagttcgccg
    14701 gtattttcca gcacaacata attagcctcc cctccggtaa tagtgaagtg agagagcttg
    14761 ttgtatcctt cgatatcagt ccctgcaccc gtgttggcaa ctaaagcact gcccgtctcc
    14821 tggttaaccc catgtgcaat accgccggta tagacaatca gcgagcctcc ggcgctaata
    14881 ttgctgccaa ttgccgtacc atctttctca acaacctgcc ggctcccgga ggatataatt
    14941 gttgtgtcag ctttcccgcc gcttttgata ttttgcgttc cgctgttgat attggtgcct
    15001 gtggctatgc cataattatt aatattctgt gtgccaccat taattatggt atttgtcgcg
    15061 tttcctgcaa catccataac cccgccatta tctattcggg tcgcatcagc tttagcattg
    15121 gttaaaactg acattgttcc tttatcttta ataatcgtct tgtttgccga accatatgcg
    15181 tttatgtcta aatgaccacc gttttccagc aacacattgt ctgccacgtg attgtggatg
    15241 gagaatgcac cttcactatt cgtaccgctc accgtcgtac cgttagtgtt agtttttaaa
    15301 attgcaccat cgtgctgggt aacatttgtt gccgtaccac cactaacatc aagcacgcca
    15361 ccagaataaa cttcaataac atccgaggtg ctggtgttat caacaatttg cgtgccacca
    15421 gaatagatat gcgtattttt tgccgttgac ttactattca gagactgagt tccgccttca
    15481 atcgtcgtgt ccagcgcacg gctctcatat actctttgct caccgccatt tttaatggtt
    15541 gttgtttcta ctgtgctctg ttcaacatac tgtcgaccac catttatggt tgtgttcgtt
    15601 gccagacttc cttgtactac gtcctgagag ccagacttat ttatcgttgt accatcagca
    15661 tgcccttgaa cttttactat ctggct
//
LOCUS       DAPHBJ010000098        15637 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.099, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000098 DAPHBJ010000000
VERSION     DAPHBJ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.099"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            77..595
                     /locus_tag="RHZ76_002753"
     CDS             77..595
                     /locus_tag="RHZ76_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HDV2813366.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            585..1811
                     /gene="dgcN"
                     /locus_tag="RHZ76_002754"
     CDS             585..1811
                     /gene="dgcN"
                     /locus_tag="RHZ76_002754"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HDV2813367.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISITSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            1827..2309
                     /locus_tag="RHZ76_002755"
     CDS             1827..2309
                     /locus_tag="RHZ76_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDV2813368.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(2385..2732)
                     /gene="rplS"
                     /locus_tag="RHZ76_002756"
     CDS             complement(2385..2732)
                     /gene="rplS"
                     /locus_tag="RHZ76_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDV2813369.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(2774..3541)
                     /gene="trmD"
                     /locus_tag="RHZ76_002757"
     CDS             complement(2774..3541)
                     /gene="trmD"
                     /locus_tag="RHZ76_002757"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDV2813370.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(3572..4120)
                     /gene="rimM"
                     /locus_tag="RHZ76_002758"
     CDS             complement(3572..4120)
                     /gene="rimM"
                     /locus_tag="RHZ76_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDV2813371.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4139..4387)
                     /gene="rpsP"
                     /locus_tag="RHZ76_002759"
     CDS             complement(4139..4387)
                     /gene="rpsP"
                     /locus_tag="RHZ76_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDV2813372.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4524..5885)
                     /gene="ffh"
                     /locus_tag="RHZ76_002760"
     CDS             complement(4524..5885)
                     /gene="ffh"
                     /locus_tag="RHZ76_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDV2813373.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6052..6843
                     /locus_tag="RHZ76_002761"
     CDS             6052..6843
                     /locus_tag="RHZ76_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HDV2813374.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6864..8150
                     /locus_tag="RHZ76_002762"
     CDS             6864..8150
                     /locus_tag="RHZ76_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDV2813375.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8205..8798)
                     /gene="grpE"
                     /locus_tag="RHZ76_002763"
     CDS             complement(8205..8798)
                     /gene="grpE"
                     /locus_tag="RHZ76_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDV2813376.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQYEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            8921..9799
                     /gene="nadK"
                     /locus_tag="RHZ76_002764"
     CDS             8921..9799
                     /gene="nadK"
                     /locus_tag="RHZ76_002764"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HDV2813377.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            9885..11546
                     /gene="recN"
                     /locus_tag="RHZ76_002765"
     CDS             9885..11546
                     /gene="recN"
                     /locus_tag="RHZ76_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDV2813378.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            11695..12036
                     /gene="bamE"
                     /locus_tag="RHZ76_002766"
     CDS             11695..12036
                     /gene="bamE"
                     /locus_tag="RHZ76_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HDV2813379.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12098..12388)
                     /locus_tag="RHZ76_002767"
     CDS             complement(12098..12388)
                     /locus_tag="RHZ76_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HDV2813380.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(12378..12815)
                     /gene="ratA"
                     /locus_tag="RHZ76_002768"
     CDS             complement(12378..12815)
                     /gene="ratA"
                     /locus_tag="RHZ76_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HDV2813381.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            12986..13468
                     /gene="smpB"
                     /locus_tag="RHZ76_002769"
     CDS             12986..13468
                     /gene="smpB"
                     /locus_tag="RHZ76_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDV2813382.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            13683..14045
                     /gene="ssrA"
                     /locus_tag="RHZ76_002770"
     tmRNA           13683..14045
                     /gene="ssrA"
                     /locus_tag="RHZ76_002770"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            14249..15490
                     /locus_tag="RHZ76_002771"
     CDS             14249..15490
                     /locus_tag="RHZ76_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDV2813383.1"
                     /translation="MARKTKPLTDTEIKAAKPKDADYQLYDGDGLTLLIKSSGSKLWQ
                     FRYYRPLTKQRTKQSFGAYPAVSLSDARKLRAESRVLLAKDIDPQEHQKEQVRNSQEA
                     KTNTFRLVAERWWNVKKASVTEDYADDIWRSLERDVLPAIGDISVTEIKAHTLVKAVH
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIHPGQ
                     LPQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWDEIDFDASEWKIPAARMKM
                     NREHTVPLSDGALAILEIMKSLSGGREFIFPSRIKPTQPMNSQTVNAALKRAGLGGVL
                     VSHGLRSIASTALNEEGFPPDVIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWADF
                     VKAADSGSIVNSGVRGIRLVG"
BASE COUNT         3952 a         4111 c         3711 g         3863 t
ORIGIN      
        1 atgaaaaaat gatattttaa tgagcattct gaaaaaatat cgatgcattt cgagcgaaga
       61 tggtggggat ccctgaatgc gtttttctca ccgactgttc ttacttctga ttctcctgct
      121 gacgggagca ccaatactcg ctcaggagcc ttctgacgtt gcgaaaaacg tgcgtatgat
      181 ggtttccggt attgtcagtt acacccgctg gcctgcgcta tcagggccac caaagttatg
      241 tatcttttca tcttcacgtt ttagtaccgc cctacaagag aatgccgcga catctttgcc
      301 ttatctgcct gtcatcattc acactcaaca agaggcgatg atttcaggct gcaatggttt
      361 ttattttggc aacgagtcac cgacatttca gatggaatta accgaacaat atccgtcaaa
      421 ggcgttatta ttaattgccg aacaaaatac cgagtgcatt attggtagcg ccttttgtct
      481 gatcatccac aataatgacg tcagatttgc cgtaaacctg gatgccttat cgcgtagcgg
      541 tgtaaaggtc aacccggatg tcttaatgct cgcacggaag aaaaatgatg gataacgata
      601 attctcttaa taagcgcccc acgtttaaaa gagcattacg caacatcagt atcaccagca
      661 tatttatcac tatgatgctg atctggttgc tgctttccgt gacctcggtg ctgaccctga
      721 aacagtacgc gcaaaaaaac ctggcactga cagcagcaac aatgacttac agtctggaag
      781 cagctgtcgt ttttgccgat ggccctgcag caactgaaac actggcagcg ctgggccagc
      841 aagggcaatt ttcaactgca gaagtacgtg ataagcagca aaatattctg gcgtcctggc
      901 attacacccg taaggatcca ggcgatactt tcagcaattt cataagccac tggctcttcc
      961 ccgcccccat cattcagccg attcgtcaca atggtgaaac cattggcgaa gtacgcttaa
     1021 ccgctcgcga cagttcaatc agccatttta tctggttttc gctcgccgta ctgaccggtt
     1081 gtattctgct ggcatcaggc atcgcaatta ccctcacccg ccatttgcac aatggcctgg
     1141 tggaagcact gaaaaatatc accgatgtcg tacatgatgt gcgttccaac cgcaattttt
     1201 cccgacgagt ttcggaagaa cgtatcgctg agtttcaccg cttcgctctc gacttcaaca
     1261 gtctgctgga tgaaatggaa gagtggcagc ttcgtttaca ggctaaaaat gcgcagcttc
     1321 tacgtaccgc gctacatgac ccattaaccg ggctggctaa ccgcgcagcg tttcgtagcg
     1381 gcatcaacac gttgatgaac aattccgatg cccgaaaaac gtcggcgtta ctatttcttg
     1441 atggcgataa tttcaaatac atcaatgata cctggggtca tgcgacgggc gatagagtct
     1501 tgattgaaat cgcaaaacgg ttagctgaag ttggcgggct gcgacataaa gcataccgcc
     1561 tgggcggcga tgaattcgct atggtgctct atgatgtaca gtcagaatct gaagtgcagc
     1621 agatatgctc agcactgaca caaatcttta atctcccgtt tgatcttcat aatggtcatc
     1681 agaccaccat gacattaagc attggttacg cgatgaccat tgagcacgcc tctgcggaaa
     1741 aattacaaga gcttgccgat cacaatatgt atcaggccaa acaccagcgt gccgaaaagc
     1801 tggtgagata acaaggatat atcgatatga taaagcacct ggtagcaccc ctgattttca
     1861 cctcactaat actgactggc tgccagtccc ctcagggaaa gtttactcct gagcaagtcg
     1921 ccgctatgca atcttatgga tttactgaat ccgccggcga ctggtcgctg ggcttatcag
     1981 atgccattct gttcgcaaaa aatgactaca aattgctccc ggaaagccag caacagatcc
     2041 aaaccatggc agccaaattg gcctcgacag ggctaacaca tgcccgtatg gatggacaca
     2101 ccgataacta tggtgaagac agttacaacg aaggcttatc attgaaacgg gcgaatgtcg
     2161 tggccgatgc atgggctatg ggtggacaaa ttccacgcag caatctcacc acacagggtt
     2221 taggaaaaaa atatcccata gccagtaaca agaccgccca gggccgcgcc gagaaccgcc
     2281 gcgtcgcagt ggtgattact accccttaaa acaccagcaa acagataaaa aagggccagc
     2341 caattggcca gcccttctta acaggatgtc gcttaagcga aatcttagtt aagacgctct
     2401 ttgatacgag cagccttacc agtacgctca cgcaggtagt acagtttagc tttacgaaca
     2461 gcaccacgac gtttgacaga aatgctgtca actaccggag agtgagtctg gaagacacgc
     2521 tcaacgcctt cgccgttgga aattttacga acagtgaatg cagagtgcag accgcggtta
     2581 cgaatagcga taaccacgcc ctcgaatgcc tgcagacgtt ttttggaacc ttcaacaacc
     2641 catactttca cttccacggt atcacccgga cggaaggaag gtacgtcctg cttcatctgc
     2701 tcttgttcaa gttgcttaat aatgttgctc ataatttaat ctcttatcct gggtaaactg
     2761 atatctcggg ggcttacgcc atcccatcat gtttatgttg ctgttgtgcg tgttccgttt
     2821 tgaactccgc cagcaacctt gcttgctctt cagtcagagc caggttttcc agaagttcag
     2881 gtcttctaag ccaggtacgg cccagcgact gtttcaaacg ccagcgacgt atctcggcat
     2941 ggttgcccga cagtaacact ggcggaactt ccatcccttc taacacctca ggccgcgtat
     3001 agtgcgggca atccagcaat ccttcagcaa aggaatcttc cgttgccgag gcttcatgtc
     3061 ccagtactcc cggaataaac cgggaaacgg agtcaatcag cgtcattgct ggtaactcac
     3121 caccactgag aacgtaatcg ccgattgacc attcttcgtc aatttcggtt tggatcacgc
     3181 gctcatctat accttcgtag cgaccgcaca ccagaatcaa tttttgattc gttgccagtt
     3241 cgctgacgcc cgcttgatca agcttgcgtc cctgtggtga cagataaatc acctttgcgc
     3301 cttcacccgc cgcggctttt gctgcatgaa tggcgtcccg caagggttgc accatcatta
     3361 acatccccgg tccgccgccg taaggacgat cgtccacggt acggtgccgg tcatgcgtga
     3421 agtcgcgagg actccagctc tggatgctca gcaggccatt tttaactgcc cggccagtta
     3481 ccccgtaatc ggtaattgcg cggaacattt caggaaacag gctaattatg ccaatccaca
     3541 tagcgccgtc ttttaccgtt tatccggtgg tttaaaaacc aggatcccaa tctacttcga
     3601 ttgaacgagt agtgagatcg actttcttga taacctgccc atcgaggaac ggtacgagac
     3661 gttccttgat accaaacgca tctttcaggt ttgccttaat gacgagaacg tcattagatc
     3721 cggtttccat catatcgacg actttaccga gatcgtagcc ttcagtggtt actacctggc
     3781 agcccatcag gtctttccag tagtagtccc cctcttcaag ctgaggcagc tgcgatgaat
     3841 ccacgacaat ttcacaattc gtcagcaggt tcgccgcatc acgatcgtca acgcctttca
     3901 gcttgatgat catgtcctga ttgtggtgct tccagctttc cagctggact tgctgccact
     3961 gacccgcctt ctggataaac cagggctgat agtcaaaaat gctttcggcg tcttcggtgg
     4021 aagaaaacac tctgagccac ccacgaatac cgtaagacga acccattttt cccaaaacga
     4081 tgggatcaac aggtgcttgc gcggtgagtt gtttgctcat catgaccacc gtgacagatt
     4141 aagctgcttt gtttacttct ttgatcagcg cagcaacgcg atcagaaata gttgcgccct
     4201 ggccaaccca gtgagcgatg cgatccagat ccaggcgagt gccttcttct ttttcgctag
     4261 cgattgggtt gaagaaacca acgcgctcga tgaagcgacc gttgcgtgca ttacggctgt
     4321 cagcgacaac aacctggtag aacggacgct ttttagcgcc gtgacgtgct aaacgaatag
     4381 ttaccataac atcctcttgt gtgaataaaa caaccggacc ccatcgagga acggagtccg
     4441 gtgtcatatt aaaagcccga aaattttact gatttctgga gaaaatgcaa tcagcagtta
     4501 ataactctgt cgcataaggt catttagcga ccaggaaatc ccggcggcat catccccttc
     4561 atgcttctca tcatcttcgc cattccgccc ttcttcattt tcttcatcat gcgctgcatg
     4621 tcgtcgaact gcttcagaag gcggttaacg tcctgcacct gcataccgca accggcagcg
     4681 atacgacgct tacgcgaacc tttgatgatt tctggcttag cgcgctcttt catcgtcatc
     4741 gagttgatga tggcttccat acgcaccagc actttatcgt ccatctgcga cttaacatta
     4801 tccgggatct gccccatgcc cggcagcttg cccatcaggc tagccatgcc gcccatattt
     4861 ttcatctggc gcagttgctc aagaaaatcg ttgagatcga agccgtcgcc ttttttcagc
     4921 ttgctggcta atttctctgc ctgcgcgcgg tcaactttgc tttcgatatc ttcgatcagc
     4981 gacagtacgt cgcccatgcc gagaatacgc gacgcgatgc ggtccggatg gaacggctcc
     5041 agcgcctcag ttttctcgcc aacaccgaga aacttgatcg gtttgccagt gatgtgacga
     5101 atagagagcg ccgcaccgcc gcgggcatcg ccgtccactt tggtcaacac tacgccggta
     5161 agcggtaacg cttcattgaa tgcttttgcc gtattggccg catcctgacc ggtcatggcg
     5221 tcaaccacaa acagggtttc aaccgggtta atcgacgcat ggacttgttt gatctcgtcc
     5281 atcatcgctt cgtcaacgtg cagacgacca gcggtatcca ccagcagcac gtcgtagaat
     5341 ttcagtttgg cttctttcag cgccgcgtta acgatatcta ccggcttctg accaacatca
     5401 gaagggaaga aatcaacgcc cacctgctct gccagcgtct caagctgttt gattgccgcc
     5461 gggcgataaa cgtcggcaga aaccaccagc actttcttct tgtgcttctc gcgcaggaac
     5521 ttaccgagtt taccaacgct ggttgtttta ccggcacctt gcaggcccgc catcagcacg
     5581 accgcaggcg gttgcgccgc caggttcagg gtctggttct cttcgcccat cgccgcaacc
     5641 agttcgttac ggacgatttt gacgaactcc tgccccggcg tcaggctctt attaacttca
     5701 tgaccaaccg ctttctcttt tacgcgattg ataaactcac gcactaccgg cagagcgacg
     5761 tccgcctcca gcagcgccat gcgcacttcg cgcagcgtat cttttacgtt gtcttcagtg
     5821 aggcgtccac ggccactgat attgcgcagc gtgcgcgaca aacgatcggt taaattatca
     5881 aacattgtct ctcgcctggg gtggaaacgg ttggccgcaa tcgcgacaca tcatcagtat
     5941 tttgccgcag tataacatga aggcgtcttt gttgttatgc aacggttgga gcagcgttca
     6001 cctgacgcta tactgcttct ctttcttatt gctcaaactg tcgacatcac tatgcccgtt
     6061 tttgctctgc tcgcgcttgt cgcctactcc gtcagtcttg cgctgattgt tcccggtctg
     6121 ctgcaaaaaa acggcggctg gcggcgcatg gctattattt ctgcggtcat tgcgctggtc
     6181 tgccacgcaa tcgctctgga agcccgcatc ctgcccgacg gtgatagcgg acaaaacctc
     6241 agcctgctga acgttggttc attggtcagt ttgatgatct gtacggtaat gaccattgtg
     6301 gcttctcgca atcgtggctg gttgctgcta cccattgtct atgcctttgc gcttatcaac
     6361 ctggcgctgg cgaccttcat gcccaatgaa tacatcaccc atctggaagc aacgcctggg
     6421 atgctggtgc acattggctt atcgctcttt tcctatgcca cgctaattat cgccgccctg
     6481 tacgcgctgc aactggcgtg gattgattac caactgaaga acaagaagct ggcatttaac
     6541 caggaaatgc cgccattgat gagtatcgag cgtaaaatgt tccacatcac gcagattggc
     6601 gtggtgctgc taacgctcac gctttgcact ggcctgttct acatgcacaa cctatttagc
     6661 atggaaaata tcgacaaggc tgtgctctct atcgtggcgt ggtttgtcta tattgtgctg
     6721 ctgtggggac attaccatga aggatggcgt ggacgccgcg tcgtctggtt taacgttgcg
     6781 ggcgcggtaa ttctgacact ggcctacttc ggcagccgaa ttgtccagca gttaatcagc
     6841 taaacccaga aaaggagttt cccctggaac acatttctac tactacgttg atcattattc
     6901 tgatcatcat ggtggtcatt tcagcctatt tttccgggtc cgaaaccgga atgatgaccc
     6961 tcaaccgcta tcgtctgcga catatggcga aacagggtaa tcgctcggcc aaacgcgtcg
     7021 aaaaattgct gcgtaagcca gaccgcctga taagcctggt gttaatcggc aataacctgg
     7081 tcaatattct tgcctccgcg ctcggcacta ttgttgggat gcgtttgtac ggcgatgcgg
     7141 gcgtggcaat tgcgactggt gtgctgactt ttgtcgtact ggtatttgct gaggtattgc
     7201 cgaaaaccat tgccgcgctg tacccggaaa aagtcgctta tccgagtagt tttctgctgg
     7261 ctccgctgca aattttgatg atgccgctgg tctggttgct gaatgctatc acccgtatgc
     7321 tgatgcgcat gatgggtatc aaaaccgata tcgtggttag cggctctttg agcaaagaag
     7381 agttgcgcac tatcgtgcac gaatcacgct cacaaatttc ccgtcgcaat caggatatgc
     7441 tgctgtcggt gctcgatctg gaaaaaatga ccgttgatga catcatggtg ccgcgcagtg
     7501 aaattatcgg tattgatatc aacgatgact ggaaatcgat tctgcgccaa ctctcccact
     7561 cacctcacgg gcgcatcgtg ctctaccgtg attcgctgga cgacgccatc agtatgctgc
     7621 gagtacgtga agcctggcgg ttgatgtcgg agaaaaaaga gttcaccaaa gaaaccatgc
     7681 tgcgcgccgc ggacgagatc tattttgtgc cggaaggtac gccgctcagc acgcagttgg
     7741 taaagtttca gcgcaacaaa aagaaagtcg gcctggtcgt caacgagtat ggagacattc
     7801 aggggctggt gacggttgaa gatattctgg aagagattgt cggcgatttc actacgtcga
     7861 tgtcgccaac acttgccgaa gaggtcacgc cgcaaaacga cggttcggtg attatcgatg
     7921 gcaccgccaa cgtgcgggaa atcaacaaag cctttaactg gcatctaccg gaagatgatg
     7981 cccgcacggt taatggcgtc attcttgagg cactggaaga gatccctgtc gcaggcaccc
     8041 gcgtgcgtat tggcgagtac gatatagata ttctcgacgt acaggacaat atgattaagc
     8101 aggtaaaagt ttttcctgtg aaaccgctgc gcgagagtgt ggcggagtaa cgaaaacggc
     8161 cctgcattcg catgcagggc cgtgaattat tacgaaaaca gaaattacgc ttttgctttc
     8221 gctacagtta ccatcgccgc acgaatcgta cgaccattca gcgtataacc cttctgcata
     8281 atgcccagta cgttacctgg cgcaacgtca tcagattcca ccattgcgat agcctgatgc
     8341 acattcgggt ccagcggcac gttagtttcg gcgatcactt caacaccaaa cttacgcaca
     8401 acatccagca tcgacttcag cgtcagctca atgccttcaa ccatcgcaga catatccggg
     8461 ttagctttat cagccacttc cagcgcacga tccaggctat caatcaccgg cagcaattcg
     8521 ttgatgaatt tctccagcgc gaatttgtgg gctttttcaa tatccagttc agtacgtcga
     8581 cgcaggtttt ccatttcggc ttttacacgc aaaatgccgt cacgttcacg ggtctgggct
     8641 tcagccagct gagcttcgag attcgcaatt ttttcatcgc gcggatccac ctgctcagca
     8701 gaagcttctg gctcaactgc ctcaatctct tcgtactgat ccatgataat ttcttccggg
     8761 gcttgcccct caggcgtttt ctgttcttta ctactcatga atttctccgc gtttttttcg
     8821 cattcatctc gctaacttcg cttattatgg ggatcagttt cagggtttca agggaagcac
     8881 tcacattgtc atcaatcttc gcaacaagga cctcggaaaa atgaataatc atttcaagtg
     8941 tattggcatt gtgggacacc cacggcaccc cactgcactg acaacacatg aaatgctcta
     9001 ccgctggctg tgcacaaaag gttacgaggt catcgttgag caacaaatcg ctcacgaact
     9061 gcaactgaag aatgtgaaaa ctggcacgct cgcggagatt gggcaactag ctgatctcgc
     9121 ggtagtcgtt ggtggcgacg gtaatatgct gggcgcggca cgcacactcg cccgttacga
     9181 tattaaagtt attggaatca accgtggcaa cctgggtttc ctgactgacc ttgaccccga
     9241 taacgcccag caacagttag ccgatgtgct ggaaggccac tacatcagcg agaaacgttt
     9301 tttgctggaa gcgcaagtct gtcagcaaga ttgccagaaa cgcatcagca ccgcgataaa
     9361 tgaagtggtg cttcatccag gcaaagtggc gcatatgatt gagttcgaag tgtatatcga
     9421 cgagatcttt gcgttttctc agcgatctga tggactaatt atttcgacgc caacaggctc
     9481 caccgcctat tccctctctg caggcggtcc tattctgacc ccctctctgg atgcgattac
     9541 cctggtgccc atgttcccgc atacgttgtc agcacgacca ctggtcataa acagcagcag
     9601 cacgatccgt ctgcgttttt cgcatcgccg taacgacctg gaaatcagtt gcgacagcca
     9661 gatagcactg ccgattcagg aaggtgaaga tgtcctgatt cgtcgctgtg attaccatct
     9721 gaatctgatt catccgaaag attacagtta tttcaacaca ttaagcacca agctcggctg
     9781 gtcaaaaaaa ttattctaat tttacgccag cctctttact gtatataaaa ccagtttata
     9841 ctgtacacaa taacagtaat ggtttttcat acaggaaaac gactatgttg gcacaactga
     9901 ccatcagcaa ctttgctatc gttcgtgagc ttgagattga ttttcatagc ggcatgaccg
     9961 taataactgg cgagaccggc gcgggtaaat ctattgcaat agatgccctc ggtctttgtc
    10021 tcggtggtcg cgctgaagcc gacatggtgc gtaccggcgc tgctcgcgct gacctgtgcg
    10081 cccgtttttc tctgaaagat acgccagcgg ctctgcgctg gctggaagaa aaccagcttg
    10141 aagacgggca tgaatgtttg cttcgtcgcg tgatcagcag cgatggtcgc tcccgtggtt
    10201 tcatcaacgg tacagctgtt cctctgtcac aactgcgcga actgggtcag ttgctgattc
    10261 agatccatgg tcagcacgct catcagttac tcaccaaacc cgagcaccaa aagttcctgc
    10321 ttgatggcta tgccaatgaa acctctctac tccaggaaat gacagcacgt tatcagttgt
    10381 ggcatcaaag ctgccgtgac ctcgcgcatc atcaacagtt aagtcaggaa cgcgctgccc
    10441 gtgcggaact gctgcaatac caattaaaag aacttaacga atttaatccg cagcccggag
    10501 agtttgaaca aatcgacgaa gagtacaaac gtctggcgaa cagcggtcaa ttgctgacca
    10561 ccagccagaa tgcattggca ttaatggccg acggtgaaga cgcaaacctg caaagtcagc
    10621 tttacacggc taaacaactg gtgagcgaat tgattggcat ggacagcaaa ctgtccggcg
    10681 tacttgatat gctggaagaa gctaccatcc agattgctga agccagcgat gaactgcgcc
    10741 actactgcga tcgtctggat ctcgatccca accgactatt tgaacttgaa cagcgcatct
    10801 caaaacagat ttcgctggca cgtaaacatc acgtcagccc tgaggcattg ccacagtatt
    10861 accagtcgct actggaagaa cagcagcaac tggacgatca ggccgactca caagaaacgc
    10921 ttgcgctggc ggtaacgaaa catcatcagc aggcactgga aatcgcgcgc gcattacacc
    10981 aacaacgcca gcaatatgca gaagaacttg cacagctgat caccgacagt atgcatgcgc
    11041 tctcaatgcc gcatgggcag tttacgatcg atgttaaatt tgacgagcat cacctgggcg
    11101 ctgacggtgc cgatcgtatt gagtttcggg taaccaccaa cccaggtcag ccaatgcagc
    11161 ctattgccaa agtcgcatcc ggtggtgaat tgtcccgcat cgcactggca atccaggtca
    11221 tcacggcgcg taaaatggaa accccggcac tgatttttga tgaagtggat gtagggatta
    11281 gcggtccaac agcggcagtt gtcggcaaac tgctgcgtca actcggcgaa tcaactcagg
    11341 tgatgtgtgt tacccacctg ccacaagtcg cgggatgtgg tcatcaacac tattttgtca
    11401 gcaaagaaac cgatggtgcg atgacagaaa cacacatgca atccctggat aaaaaagcgc
    11461 ggttacaaga actggcgcgc ctgcttggtg gcagtgaagt cacacgtaac acactggcga
    11521 atgcgaaaga actgcttgcg gcgtaaactt ttttcctgct tcacggtcag agtaaacagc
    11581 aaaacgccgt aagaccggaa agcaaaaggt tttaaagtga tgaaaggtct attatcatcg
    11641 gcatattaca gatgagccac gtactgctcg ggcccgaaaa ggaatcaaat cactatgcgc
    11701 tgtaaaacgc tgactgctgc agcagcagta ctattgatgt tgaccgcagg ctgttccact
    11761 ctggagcgag tggtttaccg tcctgacatc aaccagggga actatctgac cgctaacgac
    11821 gtatccaaaa tacgtgttgg catgacgcaa caacaagttg cgtacgcatt gggtacaccg
    11881 ctgatgtccg atccatttgg tacgaatacc tggttctatg tcttccgcca gcaaccaggt
    11941 catgaaggtg taactcagca aacgctgacg ctgaccttta acagtagcgg tgtgttgacc
    12001 aatattgata acaaacctgc gctgagtggt aactaataat aaagttgctc tcaaagacgt
    12061 taaaaaaggt gctcaatgag cacctttttt ctgtctgtta tttattcgct gatttttctg
    12121 ctcgttgcct gcgaagctct ttcggatcgg caatgagagg gcgataaatc tccacccgat
    12181 cgccatcatg cacactatcg cttagttttg ccggacggct gtaaatgccg actttgtttt
    12241 tagttaaatc gatatcggta cgcaattcca gcaagccact ggcgcgaata gcttcttcaa
    12301 ccgtcgcgcc ctcctgcagc gtcactcgct gcaggtactg cttctcaggt agcgcataag
    12361 ccacctcaac ggcaatttta cctggcactg taaacctctt tcgcacgaac cgtaaaagcc
    12421 tggaccatat tagccgccag ctctttaaac acgcgaccaa aggcgagttc aatcaactta
    12481 ttggtaaact caaagtcgag atgaaactcg atacgacacg cctcctggct cagcggcgta
    12541 aacttccatc caccaatcaa tttcttgaac ggcccatcca ccagattcat aagaatactt
    12601 tggttactgg tcaactggtt gcgggtagta aacgttttgc tgatcccagc cttagagaca
    12661 tctaccgccg cagtcatctg cccaggagtg gactccagaa tccgacttcc ggtacaaccc
    12721 ggcaaaaact gaggataaga ctgaacgtca ttcactaact gatacatttg ctccgcgctg
    12781 tagggtacca gtgcggtccg gctaatctga ggcataacaa tttccatcaa caaaaatcca
    12841 acaaataata tcatttatcc tgctgtaaaa aaaacgctat cccggcgctg ggtaacatcg
    12901 ggttcatgct aagatagagc cttgtccccc gcaggattga tatggggtgt tttcgatttc
    12961 agattaccga tgattcacga cgcttatgac gaagaaaaaa gcacataaac ctggttcagc
    13021 gaccatcgcg cttaacaagc gcgcccgtca cgaatacttt atcgaagaag agttcgaagc
    13081 gggacttgcc ctgcaaggct gggaagttaa atccctgcgc gcaggaaaag ccaatatcag
    13141 cgacagctac gtccttctgc gtgacggaga ggcatttctg tttggcgcta acatcacgcc
    13201 aatggccgtg gcctccacgc atgtggtgtg cgatcctacc cgtacccgca agttacttct
    13261 caaccagcgc gaactggact cattgtacgg tcgcgtcaat cgagaaggct ataccgtagt
    13321 ggcgctctcc ctgtactgga aaaatgcctg gtgcaaagtg aaaatcggcg tcgccaaagg
    13381 taagaaacag cacgataaac gttcagatat caaagagcgc gaatggcagg tggataaagc
    13441 acgtatcatg aaaaacgccc accgttaaac ctgcactcca attattgacc agttcctcac
    13501 cgcgcctccc tctccggcgg cgcgaatgaa catcttattg gctatcacat ccgacacaaa
    13561 tgttgccatc ccattgctta atcgaataaa aatcaggcta catgggtgct aaatctttaa
    13621 cgataacgcc attgaggctg gtcatggcgc tcataaatct ggtatactta cctttacaca
    13681 ttggggctga ttctggattc gacgggattt gcgaaaccca aggtgcatgc cgaggggcgg
    13741 ttggcctcgt aaaaagccgc aaaaaatagt cgcaaacgac gaaaactacg ctttagcagc
    13801 ttaataacct gcttagagcc ctctctccct agcctccgct cttaggacgg ggatcaagag
    13861 aggtcaaacc caaaagagat cgcgtggaag ccctgcctgg ggttgaagcg ttaaaactta
    13921 atcaggctag tttgttagtg gcgtgtccgt ccgcagctgg caagcgaatg taaagactga
    13981 ctaagcatgt agtaccgagg atgtaggaat ttcggacgcg ggttcaactc ccgccagctc
    14041 caccaaaatt ctccatcggt gattaccaga gtcatccgat gaagtcctaa gagcccgcac
    14101 ggcgcaagcc ctgcgggctt ttttgtgccc tgaatttgtc ccgcgaagtc cgaagagaac
    14161 taattaaatc cgaacctttt aggcccattg ataggcccaa cgaaaagctc tattgttttc
    14221 gttgggccta aacgcatgga gactccccat ggcaagaaaa accaagccgt taaccgatac
    14281 ggaaatcaaa gccgccaaac ctaaagatgc cgattaccag ctgtatgatg gtgacgggct
    14341 tactctgcta atcaagtcca gcggtagtaa gctctggcag ttccgttact atcgacctct
    14401 gaccaagcag cgaaccaagc agagcttcgg agcctatcca gccgtctccc tttctgatgc
    14461 gcgtaaactc agagctgaat ctcgagtttt gttggcgaaa gacattgatc ctcaggaaca
    14521 tcagaaagaa caggtgagaa attctcaaga ggccaaaacc aacactttcc ggttagttgc
    14581 cgagcgttgg tggaatgtga agaaagccag cgtaacagag gactatgccg acgatatctg
    14641 gcgctcgctt gagagggatg ttctcccggc aatcggtgat atcagtgtca ctgagattaa
    14701 ggctcatact ttggttaaag cagttcatcc ggttcaggcc agaggtgcat tagagacagt
    14761 ccgccgcctt tgtcagcgta ttaacgaagt catgatttat gcgcagaaca caggcctgat
    14821 tgatgcggtt cccagcgtaa acatcgggaa agcattcgag aaaccgcaaa agaaaaacat
    14881 gccaagcatc cacccaggtc aacttccaca gctaatgcag actatgcgta cggcaagtat
    14941 cagcctgtcc acacgatgcc tgttcatgtg gcagcttctc accatcaccc gccctgccga
    15001 agctgctgag gctcgatggg atgagatcga tttcgatgct agcgaatgga aaattccagc
    15061 tgctcgaatg aagatgaatc gggagcatac ggttccatta tctgatggag ctctcgctat
    15121 tctggaaata atgaagtctc tcagcggtgg tcgagaattt atctttccca gtcgcattaa
    15181 gcctacccag ccgatgaaca gccagacagt aaatgcagcc cttaagcgtg ctggtttagg
    15241 aggcgtactc gtttcacacg gcttacgttc tattgccagt acggcactca atgaggaagg
    15301 atttccgcct gatgttattg aggctgcact tgctcatgta gataagaatg aggtacgtcg
    15361 cgcttacaat cgaagtgact atcttgagca acgacgaccg atgatgcagt ggtgggctga
    15421 ttttgttaaa gcagcagata gcggcagtat cgtgaatagt ggggttaggg gaatacgtct
    15481 tgtaggatga atcctttatc agcacccata atgggtgctg ataactcacc tataggtaat
    15541 acggaatttt accctttggt tttctttttt ctagtaaccg tattatcgaa agccaatttg
    15601 atactctcaa tatttcttga ccagtttttt ataatgg
//
LOCUS       DAPHBJ010000099        15606 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.100, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000099 DAPHBJ010000000
VERSION     DAPHBJ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.100"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(116..868)
                     /gene="yecC"
                     /locus_tag="RHZ76_002772"
     CDS             complement(116..868)
                     /gene="yecC"
                     /locus_tag="RHZ76_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HDV2813384.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(865..1533)
                     /gene="tcyL"
                     /locus_tag="RHZ76_002773"
     CDS             complement(865..1533)
                     /gene="tcyL"
                     /locus_tag="RHZ76_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HDV2813385.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1548..2534)
                     /gene="dcyD"
                     /locus_tag="RHZ76_002774"
     CDS             complement(1548..2534)
                     /gene="dcyD"
                     /locus_tag="RHZ76_002774"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HDV2813386.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPCFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTNAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2639..3439)
                     /gene="tcyJ"
                     /locus_tag="RHZ76_002775"
     CDS             complement(2639..3439)
                     /gene="tcyJ"
                     /locus_tag="RHZ76_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV2813387.1"
                     /translation="MKLAHLGRQALMSVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3527..4078)
                     /gene="fliZ"
                     /locus_tag="RHZ76_002776"
     CDS             complement(3527..4078)
                     /gene="fliZ"
                     /locus_tag="RHZ76_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HDV2813388.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDALLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4124..4843)
                     /gene="fliA"
                     /locus_tag="RHZ76_002777"
     CDS             complement(4124..4843)
                     /gene="fliA"
                     /locus_tag="RHZ76_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HDV2813389.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5163..6845)
                     /locus_tag="RHZ76_002778"
     CDS             complement(5163..6845)
                     /locus_tag="RHZ76_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HDV2813390.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLSGFNVNGSADKASVAATADGMVKDGYIKGLTSSDGSTAYTKTTANTAA
                     KGSDILAALKTGDKITATGANSLADNATSTTYTYNATSNTFSYTADGVNQTNAAANLI
                     PAAGKTTAASVTIGGTAQNVNIDDSGNITSSDGDQLYLDSTGNLTKNQAGNPSKATVS
                     GLLGNMAADGTAVETTIKTEAGVTVTAEGNTGTDTNTDGVPDSFTDGTVKIEGATVSA
                     SAFTGIAYSANTGGNTYAVAANNTTNGFLAGDDLTRDAQTVSTYYSQADGTVTNSAGK
                     EIYKDADGVYSTENKTSKTSDPLAALDDAISSIDKFRSSLGAIQNRLDSAVTNLNNTT
                     TNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            7111..8517
                     /gene="fliD"
                     /locus_tag="RHZ76_002779"
     CDS             7111..8517
                     /gene="fliD"
                     /locus_tag="RHZ76_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDV2813391.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLAPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPISIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQNSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            8542..8952
                     /gene="fliS"
                     /locus_tag="RHZ76_002780"
     CDS             8542..8952
                     /gene="fliS"
                     /locus_tag="RHZ76_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDV2813392.1"
                     /translation="MYAAKGTQTYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSSSLIQDPV"
     gene            8952..9317
                     /gene="fliT"
                     /locus_tag="RHZ76_002781"
     CDS             8952..9317
                     /gene="fliT"
                     /locus_tag="RHZ76_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HDV2813393.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNFS"
     gene            9395..10882
                     /gene="amyA"
                     /locus_tag="RHZ76_002782"
     CDS             9395..10882
                     /gene="amyA"
                     /locus_tag="RHZ76_002782"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDV2813394.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10916..11329)
                     /locus_tag="RHZ76_002783"
     CDS             complement(10916..11329)
                     /locus_tag="RHZ76_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV2813395.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11516..12721
                     /gene="yedE"
                     /locus_tag="RHZ76_002784"
     CDS             11516..12721
                     /gene="yedE"
                     /locus_tag="RHZ76_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HDV2813396.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWNDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12718..12951
                     /gene="yedF"
                     /locus_tag="RHZ76_002785"
     CDS             12718..12951
                     /gene="yedF"
                     /locus_tag="RHZ76_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDV2813397.1"
                     /translation="MKNIIPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            13058..13717
                     /locus_tag="RHZ76_002786"
     CDS             13058..13717
                     /locus_tag="RHZ76_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HDV2813398.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRTDGKPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEVGGKEASEIATSGCVPANQFTWHPVSRAVGNVKNQGAEL
                     I"
     gene            complement(13769..14977)
                     /locus_tag="RHZ76_002787"
     CDS             complement(13769..14977)
                     /locus_tag="RHZ76_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2813399.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSSIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            14985..15398
                     /gene="tnpA"
                     /locus_tag="RHZ76_002788"
                     /pseudo
     CDS             14985..15398
                     /gene="tnpA"
                     /locus_tag="RHZ76_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         3757 a         4029 c         3866 g         3954 t
ORIGIN      
        1 gccatcacat tctcttatgt tattaatgag ttatgctgat ttgttaagca gttttatcag
       61 gcttgaaatg gcgttccgcc ccgactgtcg aatcgtcggg gctgattttt tcttattatt
      121 gcagcagaaa cttctcgagg aactggcggg tgcgaggctg ctcgggatcg gcaaataacg
      181 cttttgcggc cccctgctcg actatccgcc cctggtccat aaagatcgcc cggtccgcaa
      241 catcccgggc aaagctcatt tcgtgcgtca caatcaccat cgtgcgcttt tcctgcgcca
      301 gctgacggat ggtgttcagg acttcgccca ccagctctgg atccagcgcc gacgttggct
      361 cgtcaaacaa aatcacctca ggacgcattg ccagcgcacg tgcaatcgca acacgttgct
      421 gttgaccgcc agacaaacga cgtggatagc tggtttcttt accggccagc ccaactttcg
      481 ccaacagctc gcgagcgcgc gccgtggcct cttctttcgg ttcgcctttg acgatcaccg
      541 gcccttcaat aatgttttcc agcaccgtac gatgcggaaa caaattaaag ttctggaaaa
      601 caaacccgac gtgctgacgc aactggcgaa tcagcgattt ttgctgactt aatgaacgtg
      661 cagtatcaat agtgatatcg ccaacggtga tcgttcccgc ttcgggttgt tccagcagat
      721 ttatgctgcg tagcaacgtg gttttgccgg aaccactcgg accgataatt gccaccactt
      781 cgccaggctt tacctcaagg tcgataccgt gcagcaccgt ctgaccgtga aattttttca
      841 ccaggttctt aacttcaatg gcactcattt tggttctctc tcctggcgat taagttgatt
      901 ctcaaaatgg ttctgcaacg tcgataatac tgtcgccatg atccagtaga tgagcgaagc
      961 cgccaggtac atggtgaaca cctctagcgt gcgcgaggta atcaactgcg cctgacggaa
     1021 cagctccggc acctggattg tcgcggccag cgacgtatct tttaccaggc tgataaagct
     1081 gttcgacagc ggcggcaacg ccacccgcgc cgcctgtggc aaaatcgcgc gacgcatggt
     1141 ctgccacggc gtcataccaa tactggcagc tgcttcccac tgacctttat caatagaaga
     1201 aatggcggcg cgcagtgttt cggcggcata ggcggcagtg ttgagcgaca gaccaatcat
     1261 tgctgacgga atgggatcta attcgatacc aaactgcggc aggccgtaat agatcataaa
     1321 cagttgtgcg atgagcggtg taccacgaaa aatagagata taaaaacgcg ccagccagcg
     1381 cacgggccag attggcgaca ggcgcatcag cgcgagaata aacccgagca gtaagccaaa
     1441 aaacatgccg ccgatactga gttgcagcgt atacccagcc cctttcagca ggaacggcaa
     1501 ggaatcaata accagttgta tactttcttg catgagcgtt cccggagcta aacgtgggga
     1561 tgataggcga acagcgcagg cgcaccgccg gtatgaataa acagaatcgg cccttcatct
     1621 ttgaagcgtt tctgactgat accgtcaatc agccccgcca tcgcttttcc ggtatacaca
     1681 ggatcaagca gaatgccttc cagccgcgcc agcaatttca ctgcttccat gccttcgtcg
     1741 ttcggcacgc cgtagccagg tgcaaaatag tcatcccaga gtataatttc cgctgatgcg
     1801 gtcagctcca gttctttcgc aatcgcctgt tgtaggttaa ccactttcgg caactgatcg
     1861 gcaacggaac gcgacacggt cacgccaatc agttcgcttt caggcatcag gtgttccagc
     1921 ccaacagcca gtccggcgtg agttccggca ctgcccgatg cgactaccac cgacgaaata
     1981 ttaaccgccc cttcacactg ttgcgcgatt tccagcgcac tctccacata acctagcgcg
     2041 ccaagagcat tagaaccgcc aaccggaatg acatacgggc gaaagccttg tgcttcgact
     2101 cgcgtcgcca gctcttccag ttgggcattg ggatcggtca gtgcttcgca catttcaatc
     2161 tgggtattga acagatccag caacaagcga ttgccgttgg ttaaatagtt ttctgcgttt
     2221 gtgccaatag gattttccag cagcgccaca cagtgcagac cgagtttcgc cgcaactgcg
     2281 gcagtctgcc gcacatggtt agactggatc gccccggcag taatcagcgt atcggcacct
     2341 tcgcgcagag catctgctgc gagaaattcc agcttacgta atttattgcc gcccattgcc
     2401 atgggggtga cgtcatcccg tttgatgaaa atttcccgtc ctagataatc agaaaagcac
     2461 ggcagatatt cgagcggcgt tggcgcgccg ataaactcca accgtggaaa acgggttaaa
     2521 ttatgcagtg gcataacagc ctccgatgtg tgttgttgtg attttcttat tatgcacgct
     2581 gaaaacgcgt aaataaaaaa ggcgctagtg aaagcgccct tttttgtcat tatgctgatt
     2641 atttggtcac atcagcacca aaccattttt cggaaagagc ttgcagagtg ccgtcttttt
     2701 gcatttccgc aattgcatca ttcactgctt tcagcaggtc ttcatttcct ttacgcagcg
     2761 ccacgccaga ctcctgacgg gagaatgctt caccggttac tgccagcgta tcgttggttt
     2821 tcttcaccag atccagcgcc gccagacggt caacgaggat cgcatcgata cggcctacgc
     2881 gcagatcctg atatttggtc gggtcatcat cataggtacg cacatcgacg ccctgaacat
     2941 tctgccgcag ccactcttca tagttggtgc ccagaccgac acccactttt ttgcctttca
     3001 gatcagcagc cgttttaatg gtgccttcgt tacctttttt caccagcgcc tgaataccag
     3061 aaatggtgta cggggtagag aaatcgtatt ttttcttgcg ctcatcagaa atggtgacct
     3121 gattaatcac cacatcaatg cgtttagagt ccagcgacgc cagcataccg tcccatttgg
     3181 tcggttttag tgacgcctca acgccaagat gttttgccag ctgttgggca aattccactt
     3241 caaaaccggt taatttgccg tcatccccct gaaaactgaa cggcggataa gtgccttcca
     3301 gccctaccag cagcgtgccg cgctctttaa ctttattaag cagaccttca tctgcaaaac
     3361 ttttaacgct catgcccgca accagcgcta cggccatcac actcatcaat gcctgacgtc
     3421 ccagatgtgc taatttcata tttaccccga atgttgttat gtctgtttgc agtgtagagc
     3481 cattgtttgt aaacacaaaa acaactccgc tacatcttat tcttatttaa tatatatcag
     3541 aagcaggcag gctggaggat ttctgcacaa gtccggtaca cgtttgacgc tgatagtgtt
     3601 gatacttccg taatgcgatg cggtaattgt tggtgctggt ggtgggcagc cacggggcga
     3661 ggatttcatc aaggaaaccg tcctgcagcg gatcgaggga aatattttgc tcgtgtagat
     3721 gattccccaa acggcgcaaa cgaacgacat attcacgcac cgtacctggg ctcatttcag
     3781 tttgctcaaa aagaaattgc ttaaagccga taatatcgaa aaaatcactc tgcgttttgc
     3841 aatgtaaatc accgcaaaat cggcacaatg ccgcccatga tttttgttcc gtttgccagc
     3901 cattttcatc aagcagcgcg tccaggcggg aaatctcgat tttattcact attttgccgt
     3961 cgcgaacgag ggtaatgcga tcgagtaatt tacggcaatg cgcgcaatga gtctggctgt
     4021 gtttaaagtc tttaagatag cggcttaatg gccgtctttt caggtgctgc accatcatta
     4081 agaactcctg gtagtcaaaa ttaaagtgcg gcatttactg acgttataac ttacccagtt
     4141 tagtgcgtaa ccgtttaatg gcctggctgt gtaactgact gacccgcgat tccccgacct
     4201 ccagcaccgc gccaatctct ttgagattca gctcttcctg gtaatagagg gttaatacca
     4261 gtttttcgcg ctccggcaac gtttcgatag cttccatcac ccgctggcgc agattactgt
     4321 ccagcagttg ttgtagcggg ttttctcgct gatgatcatc agtaaccagt tcgatgctat
     4381 cgccgtgctc ttcgcgccac tcatcgtagg agaagagctg gctgttattg gtgtcgagca
     4441 acatttggcg ataatcggca atatcgatcc ctaaacgttc cgctacctca gtttccgtgg
     4501 cgttgcggcc aagttcctgc tccagttgcc ctattgcctg tgccacttca cgcgcgttgc
     4561 gtcgcacgct gcgcggcacc cagtcacggc tgcgaagttc atccagcata gcgccacgga
     4621 tacgctgcac tgcgtaagtt gtaaatgccg ttccttgtag ggcgtcataa cgttcgacgg
     4681 cattaagtaa cccaatgccg cccgcctgta gcagatcgtc aagttccacg ctcgcgggca
     4741 gtcgaacctg caggcgcaat gcttcgtgac gcaccagcgg gacataacgc tgccacagcg
     4801 agtgtttatc cattacacct tcagcggtat agagtgaatt cacgataaac agccctgcgt
     4861 tatatgagtt atcggcatga ttatccgttt ctgcagggtt tttaatcgga cgattagtgg
     4921 gtgaaatgag gggttatttg ggggttaccg gtaaattgcg ggcagaaaaa accccgccgg
     4981 tggcggggaa gcacgttgct gacaaattac cattcatgtt gccggatgcg gcgtaaacgc
     5041 cttatccggc ctacaaaaat gtgcaaattc aataaattgc aattcccctt gtaggcctga
     5101 taagcgcagc gcatcaggca atttggcgtt gccgtcagtc tcagttaatc aggttacgac
     5161 gattaaccct gcagcagaga cagaacctgt tgtggtacct ggttggcttt tgccagcacg
     5221 gagttaccgg cctgctggat gatctgcgct ttcgacatgt tggatacttc ggtcgcatag
     5281 tcagcgtcct gaatacggga ctgcgcttca gacaggttgg tagtggtgtt gttcaggttg
     5341 gtgactgcgg aatccagacg gttctggata gcacccaggg atgaacggaa tttgtcgatg
     5401 gagctgattg cgtcgtcaag cgcagccaat ggatcggaag tcttcgatgt tttattctct
     5461 gtgctgtaga caccatcagc gtctttatag atttctttgc ctgcgctatt cgtgaccgtg
     5521 ccatcggctt gcgagtagta ggttgaaaca gtttgagcat cccgggttaa gtcatccccc
     5581 gctaggaaac catttgtagt attatttgcg gcaacagcat aagtattccc accggtgttg
     5641 gcggaatatg caatgcccgt aaatgcagat gctgaaacag tagcaccttc aatttttaca
     5701 gtcccatctg tgaagctatc tggtacgcca tcagtattag tatcagtacc tgtattacct
     5761 tcagctgtaa ctgttacacc agcctctgtc ttgatggttg tttcaacagc agtaccatcc
     5821 gcagccatat ttccgagaag cccagaaacg gttgctttgc tcgggttgcc ggcctggttt
     5881 ttagtcaggt tacctgttga atccagataa agttgatcgc catcacttga agtaatattg
     5941 cccgaatcat caatatttac attctgtgct gtcccaccaa tagtaactga tgcagctgtc
     6001 gttttccctg ctgcaggtat gagatttgct gcagcattcg tttggtttac accgtcagcc
     6061 gtataggaga aggtattgct ggttgcatta taagtataag ttgtcgatgt cgcattatca
     6121 gcaaggctat ttgcacctgt tgcggtaatt ttatcgccag tcttaagcgc cgcaagaata
     6181 tcagatcctt ttgctgcagt atttgctgta gttttagtat atgcagtgct gccgtcagat
     6241 gaagttaacc ctttgatata tccgtcttta accattccgt cagctgtcgc cgcgacactt
     6301 gccttatctg cgctaccatt tacgttaaac ccactcagcc ccagcgtatc agagtcaatt
     6361 ttcttcagat caatagtgat agtctggccg tcattcgcac caacctgaat tttcatcgaa
     6421 ccgtcttttg ccagtacgtt cacgccgttg aactgggttt gaccagatac gcgatcaatt
     6481 tcgtccagac gggatttgat ttcgtcctga atggagtcca gatccgaatc agagttagtc
     6541 ccggtagagg cctgaaccgt cagttcacgg atacgctgta agttgttgtt gatttcggac
     6601 agcgcgcctt cggtggtctg tgcaacagaa ataccgtcgt tggcgttacg tgcagcctga
     6661 gtcaggcctt taatgttaga agtaaaacgg ttagcaatcg cctgacctgc ggcgtcatcc
     6721 ttcgcgctgt taatacgcaa gccagaagac agacgctcga tagaactcga cagcgcagac
     6781 tggttcttgt tgatattatt ttgagtgatc agcgagaggc tgttggtatt aatgacttgt
     6841 gccatgattc gttatcctat attgcaagtc gttgattacg tattgggttt ccacccgtcg
     6901 gctcaatcgc cgtcaaacct gttatcgtct gtcgtaaaac aacctttaga atttttttca
     6961 caaacagcca ttttttgtta gtcgacgaaa tactcttttc tctgcccctt attcccgcta
     7021 ttaaaaaaaa caattaaacg taaactttgc gcaattcagg ccgataaccc cggtattcgt
     7081 tttacgtgtc gaaagataaa aggaaatcgc atggcaagta tttcatcgct gggagtcggg
     7141 tcaggcctgg atttaagttc aatccttgat agcctcaccg ccgcgcaaaa agcgacgcta
     7201 gcccctattt caaatcagca atcgtcgttt accgctaaac ttagcgccta cggtacgctg
     7261 aaaagcgcgc tgacgacttt ccagaccgcc aatactgcat tgtctaaagc cgatcttttt
     7321 tccgccacca gcaccaccag cagcaccacc gcgttcagtg ccaccaccgc gggtaacgcc
     7381 atcgccggga aatacaccat cagcgtcacc catctggcgc aggcgcaaac cctgaccacg
     7441 cgcaccacca gagatgatac aaaaacggcg atcgccacca gcgacagcaa actcaccatt
     7501 caacaaggcg gcgacaaaga tccgatttcc attgatatca gcgcggctaa ctcgtcttta
     7561 agcgggatcc gtgatgccat caacaacgca aaagcaggcg taagcgcaag catcattaac
     7621 gtgggtaacg gtgaatatcg tctgtcagtc acatcaaatg acaccggcct tgacaatgcg
     7681 atgacactct cggtcagcgg tgatgatgcg ctacaaagtt ttatgggcta tgacgccagt
     7741 gccagcagca acggcatgga ggtctcggtc gccgcccaga atgcacagct gacggtcaat
     7801 aacgtcgcca ttgagaacag cagcaacacc atcagcgacg cgctggaaaa catcaccctg
     7861 aacctgaacg atgtcaccac aggcaaccag acgctgacca tcactcagga cacctccaaa
     7921 gcgcaaacgg cgattaaaga ctgggtgaat gcctataact cgctaataga taccttcagc
     7981 agcctgacca aatacaccgc cgtagatgcg ggagctgata gccagaattc tagcaatggc
     8041 gcactactcg gcgactccac gctgcgaacg attcagacgc agttgaagtc gatgctgagt
     8101 aataccgtca gttcttccaa ctataaaacg ctggcgcaga ttggtatcac gaccgatccc
     8161 agcgatggca aactggaact ggatgccgac aaactcaccg ctgcactgaa aaaagatgcc
     8221 agcggcgtag gtgcattgat tgttggcgat ggtaaaaaaa ccggcatcac gaccaccatc
     8281 ggcagcaacc tgaccagttg gctttcgaca acgggcatta ttaaagccgc taccgatggc
     8341 gttagtaaga ccctgaataa attaactaaa gactacaacg ccgccagcga tcgcattgat
     8401 gcgcaggtcg cgcgctataa agaacaattt acccaactgg acgttttaat gacctcgtta
     8461 aacagcacca gcagctactt aacgcagcag ttcgaaaaca acagtaattc caagtaatca
     8521 atattcatcg ggagacaggt catgtacgcg gcaaaaggca cccagaccta tgcacaaatt
     8581 ggcgtcgaaa gcgccgtaat gagcgccagc cagcagcagc tggtcaccat gctatttgat
     8641 ggagtgctga gcgcactggt tagagcgcgc ctgtttatgc aggataacaa tcagcaaggc
     8701 aaaggcgtct ctttgtcaaa agcgatcaac atcattgaga acggactgcg ggtgagtctt
     8761 gatgaagaga gcaaagacga attaacccaa aacttgattg ctctttatag ctatatggtc
     8821 aggcgcttgc tgcaagccaa tttacgcaac gatgtctccg cagtcgaaga agtggaagca
     8881 ttaatgcgca atattgccga tgcctggaaa gagtcgttac tctcctcttc tttgattcag
     8941 gacccagtct gatgaaccat gcaccgcatt tgtatttcgc ctggcaacaa ctcgtcgaaa
     9001 aaagccagct catgttacgc ctggcaacgg aagaacaatg ggacgaactc atcgccagcg
     9061 aaatggcgta tgtgaatgcg gtgcaggaga ttgcacattt gactgaagag gttgacccgt
     9121 ccaccacgat gcaggagcag ctccgcccga tgctgcgcct gattctcgac aacgaaagca
     9181 aggtaaagca gttattacag attcgtatgg atgaactggc gaaactggtc ggtcagtcat
     9241 cggtgcaaaa atcggtgtta agtgcctatg gcgatcaggg cggctttgtg ctggctccgc
     9301 aagataactt ctcgtgaatc ttaacgagct aatggctcgc gaataatccg attacggcta
     9361 cgcttcaaat gttccccttg aatggagtcg aagaatgcgt aatcccacgc tgttacaatg
     9421 ttttcactgg tattacccgg aaggcggtaa gctctggcct gaactggccg agcgcgccga
     9481 cggttttaat gatattggta tcaatatggt ctggttgccg cccgcctata aaggtgcatc
     9541 gggcgggtat tcggtcggct acgactccta tgatttattt gatttaggcg agtttgatca
     9601 gaaaggcagc atccctacta aatatggcga taaagcacaa ctgctggccg ccattgatgc
     9661 tctgaaacgt aatgacattg cggtgctgtt ggacgtggta gtcaaccaca aaatgggcgc
     9721 ggatgaaaaa gaagctattc gcgtgcagcg tgtaaatgct gatgaccgta cgcaaattga
     9781 cgaagaaatc attgagtgcg aaggctggac gcgttacacc ttccccgccc gtgccgggca
     9841 atactcgcag tttatctggg atttcaaatg ttttagcggt atcgaccata tcgaaaatcc
     9901 tgatgaagat ggcatattta aaattgttaa cgactacacc ggcgaaggct ggaacgatca
     9961 ggttgatgat gaactcggta atttcgatta tctgatgggt gagaatatcg atttccgcaa
    10021 tcatgccgtg acggaagaga ttaaatactg ggcgcgctgg gtgatggaac aaacacaatg
    10081 cgacggtttt cgtcttgatg cggtcaaaca tattccggcg tggttttata aagagtggat
    10141 cgaacacgta caggaagttg cgccaaagcc gctgtttatt gtggcagagt actggtcgca
    10201 tgaagtcgat aaactgcaaa cgtatattga tcaggtggaa ggcaaaacca tgctgttcga
    10261 tgcgccgctg cagatgaaat tccatgaagc atcgcgcatg ggacgcgact acgacatgac
    10321 gcagattttc actgggacgc tggtggaagc cgatcctttt cacgccgtaa ccctcgttgc
    10381 caaccatgac acccaaccgt tgcaagccct cgaagcgcca gtcgaaccgt ggtttaaacc
    10441 gctggcgtac gccttaattt tgttgcggga aaatggcgtt ccttcggtat tctatccgga
    10501 cctctacggt gcgcattacg aagatgtcgg tggtgacggg caaacctatc cgatagatat
    10561 gccaataatc gaacagcttg atgagttaat tctcgcccgt cagcgtttcg cccacggtgt
    10621 acagacgtta tttttcgacc atccgaactg cattgccttt agccgcagtg gcaccgacga
    10681 atatcccggc tgcgtggtgg tcatgtcgaa cggggatgat ggcgaaaaaa ccattcattt
    10741 aggagagaat tacggcaata aaacctggcg tgattttctt gggaaccggc aagagagcgt
    10801 agtgaccgac gaaaacggcg aagcaacctt cttttgcaac ggtggcagcg tcagcgtgtg
    10861 ggttatcgaa gaggtgattt aaattcatcc ccggcggcaa gccggggaga tttcattacg
    10921 gcagtggcgt cggtaacggc gctttatcca gcgcggcggc acactctgcc gtcgggcgat
    10981 ccacacgctc catcgtcata ccatcgtact caatagtgtt accgtcacgc tcaacctcgt
    11041 acagctcacg ttttacggta acgttagtga gatcatcaga catcaatgtc aacttacccg
    11101 gcacggcaat cacccgctgc cactgacggc aatccagggt atcgccctct tttgtgacaa
    11161 tcaggctgcc tatcgcttcc gggctgacca gcgcacgttg cggccctttg gtctgccagt
    11221 agcccgccag ccagtccggc gcgggggttt tcacgacatt gttatagttt tcgacttcgg
    11281 cacagcccgc cagcaacatg agcgcgcctg caattgcgag ttttttcatc attcgtcctg
    11341 catgagaaga aaagaggatt gtggcattaa agccctgatg tcgccagcct ttccaggttc
    11401 agagagaaat ttgatctaac tattattttt gtgaattatt accccgaaaa taaatttgca
    11461 tgatgtatga ttcgcgactc ttgtttcaaa ccttctgagt tcagaggcta cattcatgtc
    11521 atggcagcaa ttcaaacacg cctggttgat taaattctgg gcacccatcc ctgcggtcat
    11581 cgcggcgggt attctctcca cttactattt tggcattact ggcacttttt gggctgtcac
    11641 gggtgaattt acccgttggg gcggtcagct cctgcagctg tttggcgtcc atgctgaaga
    11701 gtggggttat tttaaaatta tccatctgga aggatcgcca ttaacccgca tcgacgggat
    11761 gatgatcctc ggcatgtttg gcggctgctt tgccgcagcg ctgtgggcca acaatgtcaa
    11821 actacgcatg ccgcgcagcc gtatccgcat tatgcaggcc attattggcg gcattatcgc
    11881 cggttttggc gcgcgtctgg caatgggctg taacctggcg gcgttcttta ccggcattcc
    11941 tcagttctcg ctgcatgcct ggttctttgc catcgccact gccattggtt catggtttgg
    12001 cgcacgcttt accctgctgc ccatcttccg tattcccgtg aaaatgcaga aagtttctgc
    12061 cgcctcaccg ctgacgcaaa aaccggatca ggcgcggcgt cgttttcgtc tcgggatgct
    12121 ggtctttttc ggtatgctgg gctgggcgct gctcacggcg atgaaccaac caaaactggg
    12181 gctggcaatg ctgtttggcg tcggctttgg tttactgatt gaacgtgcgc aaatctgctt
    12241 tacttcggcg ttccgcgata tgtggatcac cggacgtacc catatggcga aagcaatcat
    12301 tatcggtatg gcggtgagtg ccatcgggat cttcagttac gtacagttag gcgttgaacc
    12361 caaaatcatg tgggcgggac caaacgcggt aattggtggt ttactgtttg gttttggcat
    12421 cgtgctggca ggcggctgcg aaaccggctg gatgtaccgc gcggtagaag gccaggtgca
    12481 ctactggtgg gtcggtctgg gcaatgtgat cggctcgacg attctggcgt attactggaa
    12541 tgatttcgct ccggcgctgg ccaccgactg ggacaaaatc aacctgctga aaacctttgg
    12601 cccgatgggt ggcctgctgg tgacatattt gctgttgttt actgcgctga tgttgattat
    12661 cggctgggaa aaacgcttct tccgccgtgc ggcaccgcag actgctaagg agatcgcatg
    12721 aaaaatatca ttcctgatta ccgtcttgat atggtgggtg aaccctgccc ttatccggca
    12781 gtcgcaaccc ttgaggcgat gccgcagtta aaaaaaggtg agatcctgga agtggtgagc
    12841 gactgtccac agtcgatcaa taatattcca ctggatgcgc gtaatcacgg ctatacggtg
    12901 ctggatattc agcaagacgg gccgaccatt cgttatttaa ttcagaagta atccctcatt
    12961 cccgctgtgg cccacagcgg gaacatcgtt gcctctgaca catagatttt acccgccccc
    13021 tctcctattc ttttcagtat gatctcagga ggtttctatg tgtggacgct ttgcccaatc
    13081 ccaaacgcgt gaagattacc ttgcgcttct cgcggaagat attgaacgcg atattcccta
    13141 cgatcccgaa cccattggca gatacaacgt cgcgccggga accaaagttc ttctgctcag
    13201 tgaacgtgat gaacaccttc atctggatcc ggttttctgg ggatatgctc ccggatggtg
    13261 ggataaaccg ccgctgatta acgcccgcgt agaaactgcc gcaaccagtc gcatgtttaa
    13321 accgctctgg caacacggtc gggcaatctg ttttgccgat ggctggtttg agtggaaaaa
    13381 agaaggcgac aaaaagcagc cttattttat ctatcgtact gacggaaaac ctgtttttat
    13441 ggccgcgata ggaagcacac catttgagcg tggtgacgaa gccgaaggat ttttgatcgt
    13501 cactgcggcg gcagatcaag gtctggtaga tattcatgac cgccgcccgc tggttctgtc
    13561 gccagaagct gcacgggaat ggatgcggca agaggttggc ggtaaagaag cctcagaaat
    13621 tgctaccagt ggatgtgtgc cagcaaacca gttcacctgg cacccggtat cgcgcgcggt
    13681 aggtaatgtc aaaaaccagg gcgcggagtt aatttaacct gtttgttgac atcctccacg
    13741 ccctgaagga cgaggatccc tgctacgttc aggctgtcgc ctgaatcatt tcggtgggtt
    13801 cctgcttcaa cgggcggcct gactgcacca tctctccaca ggcaagaacg gcgtgccccg
    13861 ccgctaaaat gttacgagcg ccatttacat cggcgttcgc tgtatatcca catacctggc
    13921 atctgaattt actttgtgac aggcggtttt ctttcgctgt atgaccacag cacgcgcaac
    13981 gctggcttgt gtacgctggc ggaacagcaa gcacctgacc gccacgccag agctgcttgt
    14041 actcaagctg gcggcgcatt tcataccagc cctgatccag tatcgaacgg tttaaacctg
    14101 attttgcccg gacattgcgc cccggctggc tgaccgtacc cgctgctgac tttgacatgt
    14161 tgctgacctt caaatcctca atgactatca ttgcgtggtt tttgctgacg gtcgttgtga
    14221 ctttgtgaag gtagtccctg cggatatttg cgatacagga atgcagtcgc tgtattttgc
    14281 gtttctgctt ctgccagttg ttgctgaatt tgaccttgcg gcttaactgt cgctgaagtc
    14341 tcgccagctt cttctggttt ttctgaaaac tgtttacagg ctcaaagact gtgccatctg
    14401 acagcgtggc gagtttagcc acgccagcat ccagcccgac cattgatgct gaagggtgaa
    14461 ccggagtgga tacttcactt tctgtctgaa tactgatgta ccacttaccg caggactggc
    14521 tgacagtgac atttttcaca acacccgtga cctgacggct gttccggtag cgcatccagc
    14581 ccagtttcgg cagaaaaata ctgctgtttt cctgatcgag cttaacgccc tgcgggtagc
    14641 ggaatacatc attctgtccc cgctttttga atcggggaaa agcagcccga ttctggaaga
    14701 agtttttgta agcccgctca aggtctttca gtgactgttg caatggctgt gacggggcat
    14761 ctttaagcca ttgcgtttca gtggcatttt tccactcaac cagccaggaa gccattttcc
    14821 cgtaagggat gtatttatta ccggcctcat gattctcatt ctgacgtgcc agagcacgat
    14881 tgaaaacgaa acgacatgcg cctgcgaagc gcctcatttc acactcctgt tgaccacctg
    14941 gtcttaactg gaatttaaat gcctgtaatc gtttcacagg tcaaatgata ctttggtctg
    15001 tgaaaaaaga aaccgatatc cgacggggaa gcattgtgtt ttcctgatgc atgttcacct
    15061 ggtctttgtc accagatacc gacgccagat ttttgaccat gacgcgacag aaaaactacg
    15121 cacttacttt tcaaatgtat gtgctgattt tgaagctgaa ctggttgaaa tggatggcga
    15181 accagatcac gtccatttgt taattaatta tcctcccaaa ctggcgatat ccagtctggt
    15241 aaacagcctc aaaggcgtat cgggtaggtt actgcgacga gatcgaccag atattgcagt
    15301 caggtattac tacaaaggcg ttttgtggag tgctggctat tttgccagta gctgcggagg
    15361 tgcgccaata cattgaacaa cagcaaacac ctggttaggt ggaaaaccgc gccttatatc
    15421 cctgccctga aggacggggt tttacggcgc accggataag cctggagatt atctacacca
    15481 gcatggacat cattgtacta tgccccgtac atgccccatc acatcctcaa catcaaagta
    15541 ttagagcctt gcaaaccatt atgcgtctca gtttgtctca tctatcttac gcaaaaaaaa
    15601 tagaac
//
LOCUS       DAPHBJ010000100        15512 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.101, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000100 DAPHBJ010000000
VERSION     DAPHBJ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.101"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            3..884
                     /locus_tag="RHZ76_002789"
     CDS             3..884
                     /locus_tag="RHZ76_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2813400.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            1089..2558
                     /gene="lysP"
                     /locus_tag="RHZ76_002790"
     CDS             1089..2558
                     /gene="lysP"
                     /locus_tag="RHZ76_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HDV2813401.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGEVAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            2852..4843
                     /gene="cirA"
                     /locus_tag="RHZ76_002791"
     CDS             2852..4843
                     /gene="cirA"
                     /locus_tag="RHZ76_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HDV2813402.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(4875..5711)
                     /gene="yeiG"
                     /locus_tag="RHZ76_002792"
     CDS             complement(4875..5711)
                     /gene="yeiG"
                     /locus_tag="RHZ76_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDV2813403.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            5969..6637
                     /gene="folE"
                     /locus_tag="RHZ76_002793"
     CDS             5969..6637
                     /gene="folE"
                     /locus_tag="RHZ76_002793"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDV2813404.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            6654..7811
                     /locus_tag="RHZ76_002794"
     CDS             6654..7811
                     /locus_tag="RHZ76_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HDV2813405.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLVGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVDAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWRLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRFFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            7953..8993
                     /gene="galS"
                     /locus_tag="RHZ76_002795"
     CDS             7953..8993
                     /gene="galS"
                     /locus_tag="RHZ76_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HDV2813406.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            9273..10271
                     /gene="mglB"
                     /locus_tag="RHZ76_002796"
     CDS             9273..10271
                     /gene="mglB"
                     /locus_tag="RHZ76_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDV2813407.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            10332..11852
                     /gene="mglA"
                     /locus_tag="RHZ76_002797"
     CDS             10332..11852
                     /gene="mglA"
                     /locus_tag="RHZ76_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HDV2813408.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            11868..12878
                     /gene="mglC"
                     /locus_tag="RHZ76_002798"
     CDS             11868..12878
                     /gene="mglC"
                     /locus_tag="RHZ76_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HDV2813409.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(12949..14184)
                     /gene="preA"
                     /locus_tag="RHZ76_002799"
     CDS             complement(12949..14184)
                     /gene="preA"
                     /locus_tag="RHZ76_002799"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HDV2813410.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(14178..15416)
                     /gene="preT"
                     /locus_tag="RHZ76_002800"
     CDS             complement(14178..15416)
                     /gene="preT"
                     /locus_tag="RHZ76_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HDV2813411.1"
                     /translation="MPQQNYLDELTPAFTSLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MVADKIILAVGQHARLDAFAKLEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
BASE COUNT         3673 a         3821 c         4089 g         3929 t
ORIGIN      
        1 ctatgcacat caccctccgg cagttggaag tttttgcaga agtattgaaa agtggatcaa
       61 ccacccaggc gtcggtgatg ctggcgttgt cgcaatcagc agtgagcgca gccttgaccg
      121 acctggaagg gcagcttggc gtgcaactgt ttgatcgcgt ggggaaaaga ctggttgtta
      181 atgaacacgg gcggctgctc tatccgcgtg cgttggcatt gcttgaacag gcggttgaaa
      241 tcgaacaact gtttcgcgaa gacaacggcg cgattcgtat ctatgccagt agtaccatcg
      301 gtaactacat tctgcctgca gttatcgccc gttatcgcca tgattatccg cagttgccga
      361 ttgaacttag cgttgggaat agccaggacg tgatgcaagc ggtgctggat ttccgcgttg
      421 atattggctt tattgaagga ccgtgccaca gcactgaaat catttctgaa ccgtggctgg
      481 aagacgagct ggtggttttc gccgcgccga cttcgccgtt ggcccgtggt ccggtcacct
      541 tagaacagct ggccgctgcg ccgtggatcc tgcgtgaacg cggttccggc acgcgggaga
      601 ttgtcgatta tctgttgctg tcacatttac cgaagtttga gatggcgatg gaattaggta
      661 actccgaggc aatcaaacat gcggtgcgtc atgggttggg aattagttgc ctgtcgcgac
      721 gtgtgattga agatcaattg caggcaggca cattaagtga agttgcggtc cctctgccgc
      781 gcctgatgcg tacgttgtgg cgtatacatc atcggcaaaa acacctttcc aacgcgctac
      841 ggcgctttct ggactattgc gatcccgcaa atgtgccgcg ttaagttgct gtacaagaac
      901 atgctggtgc tgtgtcgatt tcgtgacgca gcgccttcag catgcattcg ccagaaaaga
      961 gattggctgc tttacttata atccctgggc gatcatgaag gtgtcttata accgtgtatt
     1021 tctgccggaa ggattgccaa tcgtctgcta caatcgcgcc tcatttttaa gatggatagc
     1081 atttttgtat ggtttccgaa actaaaacca cagaagcgcc gggcttacgc cgtgaattaa
     1141 aggcgcgtca cctgacgatg attgccattg gcggttccat cggtacaggt ctttttgttg
     1201 cctctggcgc aacgatttct caggcaggtc cgggcggggc attgctctcg tatatgctga
     1261 ttggcctgat ggtttacttc ctgatgacca gtctcggtga agtggctgca tatatgccgg
     1321 tttccggttc gtttgccact tacggtcaga actatgttga agaaggcttt ggcttcgcgc
     1381 tgggctggaa ctactggtac aactgggcgg tgactatcgc cgttgacctg gttgcagctc
     1441 agctggtcat gagctggtgg ttcccggata caccgggctg gatctggagt gcgttgttcc
     1501 tcggcgttat cttcctgctg aactacatct cagttcgtgg ctttggtgaa gcggaatact
     1561 ggttctcact gatcaaagtc acgacagtta ttgtctttat catcgttggc gtgctgatga
     1621 ttatcggtat cttcaaaggc gcgcagcctg cgggctggag caactggaca atcggcgaag
     1681 cgccgtttgc tggtggtttt gcggcgatga tcggcgtagc tatgattgtc ggcttctctt
     1741 tccagggaac cgagctgatc ggtattgctg caggcgagtc cgaagatccg gcgaaaaaca
     1801 ttccacgcgc ggtacgtcag gtgttctggc gaatcctgtt gttctatgtg ttcgcgatcc
     1861 tgattatcag cctgattatt ccgtacaccg atccgagcct gctgcgtaac gatgttaaag
     1921 acatcagcgt tagtccgttc accctggtgt tccagcacgc gggtctgctc tctgcggcgg
     1981 cggtgatgaa cgcagttatt ctgacggcgg tgctgtcagc gggtaactcc ggtatgtatg
     2041 cgtctactcg tatgctgtac accctggcgt gtgacggtaa agcgccgcgc attttcgcta
     2101 aactgtcgcg tggtggcgtg ccgcgtaatg cgctgtatgc gacgacggtg attgccggtc
     2161 tgtgcttcct gacctccatg tttggcaacc agacggtata cctgtggctg ctgaacacct
     2221 ccgggatgac gggttttatc gcctggctgg ggattgccat tagccactat cgcttccgtc
     2281 gcggttacgt attgcaggga cacgacatta acgatctgcc gtaccgttca ggtttcttcc
     2341 cactggggcc gatcttcgca ttcattctgt gtctgattat cactttgggc cagaactacg
     2401 aagcgttcct gaaagatact attgactggg gcggcgtagc ggcaacgtat attggtatcc
     2461 cgctgttcct gattatttgg ttcggctaca agctgattaa aggaactcac ttcgtacgct
     2521 acagcgaaat gaagttcccg cagaacgata agaaataagt ttcctccctt ccttgctaag
     2581 ccctctcaac cgagagggct ttttcaattc catttccctg acaaatcatg cggatataaa
     2641 atttaacatt tggattgata attgttatcg tttgcattat cgttacgccg caatcaaaaa
     2701 aggctgacaa atcagaggct gttccggctt tctgggatga tcacctgcat aaaaaataag
     2761 tccaccgcga tgctgccgta cgcaagggga cgtgaagaag atgtgagcga taacccattt
     2821 tattttcgta gttacctcat ggagatatgg aatgtttagg ttgaaccctt tcgtacgggt
     2881 cgggctgtgt ttgtccgcta tttcttgtgc atggcctgtg ttagcggtcg atgatgatgg
     2941 cgaaacgatg gttgtcactg catcttccgt ggaacaaaat cttaaagatg cacctgccag
     3001 tatcagcgtc attacccagg aagacctgca gcgaaaaccg gtacagaatc tgaaggatgt
     3061 cctcaaagaa gtgcctggcg tacaactgac gaacgaaggg gataaccgta agggcgttag
     3121 tattcgtggt ctggacagca gctataccct gattctcgtc gacggtaaac gcgtgaactc
     3181 ccgcaatgcc gtcttccgcc acaatgattt cgatctgaac tggatcccgg tcgattccat
     3241 cgaacgtatt gaagtggtcc gtggcccgat gtcgtcgctg tacggttccg atgcgctcgg
     3301 cggtgtagtg aatatcatca ccaaaaaaat cggtcagaaa tggtcgggta ccgttaccgt
     3361 cgataccacc attcaggaac atcgcgatcg cggtgacacc tataacggtc agttctttac
     3421 cagtggacca ttaattgatg gtgtgctggg aatgaaagct tacggcagcc tggcaaaacg
     3481 tgaaaaggat gacccgcaaa actcaacgac caccgatacc ggagaaacgc cgcgtattga
     3541 aggattctcc agccgcgacg gcaatgtcga atttgcctgg acaccgaatc aaaatcacga
     3601 ttttactgcc ggatacggtt tcgaccgtca ggatcgtgat tccgactcgc tggacaaaaa
     3661 ccgcctggaa cgccagaact actccgtcag ccataatggg cgttgggatt acggcaccag
     3721 cgaactgaaa tactacggtg agaaagtcga gaacaaaaac cctggcaaca gcagcccgat
     3781 aacttccgaa agcaatacgg tcgacggcaa atacacgttg ccgctgacgg cgattaatca
     3841 gtttctcacg gttggcggtg aatggcgtca cgacaaactt agcgatgcgg tgaacctgac
     3901 cgggggaacc agctccaaaa cgtctgccag ccagtacgcg ctgtttgtgg aagatgaatg
     3961 gcggatcttc gagccgctgg cgctgacgac cggcgtgcgt atggacgatc acgaaaccta
     4021 cggtgaacac tggagtccgc gtgcctacct ggtttataac gccaccgaca ccgtaacggt
     4081 gaaagggggc tgggcgacgg catttaaagc accttctctg ttgcaactta gccctgactg
     4141 gacgagcaat tcctgccgtg gcgcatgtaa gattgtgggt agcccggatc tgaaaccaga
     4201 aaccagcgaa agttgggagc tggggcttta ctacatgggt gaagaaggct ggctggaagg
     4261 ggttgaatcc agcgttaccg ttttccgtaa cgatgtgaaa gatcgtatca gcatcagccg
     4321 tacgtctgac gtcaacgctg caccgggcta ccaaaacttt gttggttttg agacgggcgc
     4381 taacggacgg cgcataccgg tatttagcta ctacaacgtt aacaaagctc gtattcaggg
     4441 cgtggaaacc gaactgaaaa ttccgttcaa cgatgaatgg aaactgtcga tcaactacac
     4501 ctacaacgat ggtcgtgatg tcagcaacgg cgaaaacaaa ccgctatccg atctgccgtt
     4561 ccatactgct aacggtacgc tggactggaa accgctggcg ctggaagact ggtcattcta
     4621 tgtttctggg cactataccg ggcagaaacg cgccgacagc gcgacggcta aaacaccggg
     4681 cggttatacc atctggaata ccggcgcggc ctggcaggtg actaaagacg tcaaactgcg
     4741 cgcaggcgtg ctgaaccttg gcgacaagga tctcagtcgt gacgactaca gctataacga
     4801 agacggacgt cgttacttta tggcagtgga ttatcgcttc tgatgagaag atgcccggcg
     4861 aaccgggcgg actttcactt cagtaaatac tgcgcatgga agcgcaggtg atcctctata
     4921 aaagaggcga tgaagtagta actgtgatca tatcccggct gaatacgcag cgtcatcggc
     4981 cacgctttct ggcgcgcggc ttctgccagt accgcaggtt gcaactggtc ggcaagaaac
     5041 tgatcattat cgccctgatc gataagcgtc gggatcgcat cctgcgcgtt actggcatac
     5101 atcagtgcgc aactgtccca ttccagccat gcatttttgt cctcacctaa atagctgcta
     5161 aacgctttga ttccccacgg gacgctgcac ggattcacaa ttggcgcaaa ggccgaaacg
     5221 ctggtgtatt tacccggatt tttcagcgcc ataatcagcg caccgtgacc acccattgag
     5281 tgaccgctaa tggcgcagcg gtcgctgaca ttaaattgcg actgaaccag cgccggtaat
     5341 tcatcgcgca gataatcata catccggtaa tgcgtcgccc acggcggttg cgtggcatta
     5401 agataaaagc ctgcgccctg gcctaaatcg tagccatcgt cgttggcaac cttttcgccg
     5461 cgcgggctgg tgtctggcat caccagtaca atccccagtt ccgccgctac ccgctgggca
     5521 cccgccttgg tggtgaagtt ctcgtcattg caggttaatc cggaaagcca gtacagcact
     5581 ggtggcggag tgtgatcacg aggtggaggg agaaagatac tgaacgtcat cgggcagttt
     5641 aaggtactgg agtcgtgtcg ccagcgttgc tgccagcctt caaaacagcg gtgctcttcg
     5701 agcatttcca tgcgtggctc cttgttgtgt tgtttgcaat ttcttcatca taccgtttat
     5761 tcaatgtgct gtgagtaact ttcacttccg tatttgcata acgatgtttt aacatctgct
     5821 gatgaaaggc agcggcaatt acaataatta tcgctgtgaa tactggatta tgtgcgccgc
     5881 ctcacgcaca ataatcaggc tgtaaatcag cttaataact ttgcccccac gcagggcgga
     5941 ggcgtcacac ctgcaggaga aatcataaat gccatcactc agtaaagaag cggccctggt
     6001 tcatgaagcg ttagttgcgc gaggactgga aacaccgctg cgcccgcccg tgcatgaaat
     6061 ggataacgaa acgcgcaaaa gccttattgc tggtcatatg accgaaatca tgcagctgct
     6121 gaatctcgac ctggctgatg acagtttgat ggaaacgccg catcgcatcg ctaaaatgta
     6181 tgtcgatgaa attttctccg gtctggatta cgccaacttc ccgaaaatca ccctcattga
     6241 aaacaaaatg aaggtcgatg aaatggtcac cgtgcgcgat atcactctga ccagtacctg
     6301 tgaacaccat tttgttacca tcgatggcaa agcgacggtg gcttatatcc cgaaagattc
     6361 ggtgatcggt ctgtcaaaaa ttaaccgtat cgtgcagttc tttgcccagc gtccgcaggt
     6421 gcaggaacgt ctgacgcagc aaattcttat tgcgctacaa acgctgctgg gcaccaataa
     6481 cgtggctgtc tcgatcgacg cggtgcatta ctgcgtgaaa gcgcgtggca tccgcgatgc
     6541 aaccagtgcc acgacaacga cctctcttgg tggattgttc aaatccagtc agaatacgcg
     6601 ccacgagttt ctgcgcgctg tgcgtcatca caactgatta aaaggcagga accatggagc
     6661 gcaacgtcac gctcgatttt gttcgcggcg tcgccattct ggggatcctg ctattaaaca
     6721 tcagcgcctt tgggctacca aaggcggctt atcttaatcc cgcctggtac ggcgctatta
     6781 cgccgcagga tgcatggacc tgggcatttc ttgatctcgt cggccaggtg aaattcctca
     6841 cgctttttgc gctgctgttt ggtgcgggcc tgcaaatgtt gctgccccgt ggcagacgct
     6901 ggatccagtc gcggttaacg ctgttagtct tgttgggctt tattcacggt ttactgttct
     6961 gggacggcga tattctgctg gcttacgggc tggtgggctt aatctgctgg cggctggtgc
     7021 gcgatgcgcc atcggtaaaa agcctgttta atacaggcgt catgctttat ctggtggggc
     7081 ttggcgtttt gctgttattg gggttgattt ccgatagcca gaccagccgc gcctggacgc
     7141 cggatgcatc ggctattttg tatgaaaaat actggaagct tcacggcggc gttgatgcga
     7201 tcagtaatcg tgccgatggt gttggcaaca gtttactggc actgggcgca cagtatggct
     7261 ggcaactggc tgggatgatg ctcattggtg ccgcattgat gcgcagtggc tggctgaaag
     7321 ggcagttcag cttacgtcac tatcgtcgta ctggttttgt gctggtggcg attggggtga
     7381 tcattaacct tcctgccatc gccctgcaat ggcggctgga ctgggcatat cgctggtgcg
     7441 ccttcttact tcagatgccg cgggaactga gtgcgccgtt tcaggcgata ggctatgcgt
     7501 cgctgtttta tggtttctgg ccgcaattga gccgctttaa gctggtgctt gcgatcgcct
     7561 gcgtcggacg gatggcgctg accaactatc tattgcaaac gctgatttgt accacgcttt
     7621 tttaccacct cggtttgttt atgcattttg accgcctgga gctgctggcg tttgttattc
     7681 cggtatggct ggcgaatatc ctcttctctg ttatctggct gcgtttcttc cgccaggggc
     7741 cggtggaatg gctctggcgt cagttaactt tgcgtgctgc cggaccggca atatctaaaa
     7801 catcaagata acgatctgga tcacattcgt taacaaaacg gctgtaaccg tttccattgc
     7861 tgtgactcga ttcacgaagt cctgtattca gtgctgacaa aatagccgcc agcaagcagt
     7921 catttactgc aatctcataa caggtagtga atatgatcac cattcgtgat gtagcgcgtc
     7981 aggctggcgt ctctgtggca acggtttccc gggtgctcaa taacagcacg ctggtcagtg
     8041 ccgacacgcg tgaagcagta atgaaagccg tgagtgagct ggattatcgg ccaaacgcca
     8101 atgcccaggc gctggcaact caggttagcg acaccattgg cgtggtggtg atggacgttt
     8161 ctgatgcgtt tttcggcgcg ctggtaaaag cggtggatct agtcgctcag cagcatcaga
     8221 aatacgtgct aatcggcaat agctatcatg aagcggaaaa agagcgtcac gccattgagg
     8281 tgttaattcg ccagcgttgt aatgcgttga ttgttcactc aaaagcattg agtgacgatg
     8341 aactggcgca atttatggat aacattcccg gtatggtgtt aatcaaccgc gttgtgccgg
     8401 ggtacgccca tcgttgcgtt tgcctggata atctcagcgg tgcccgaatg gcgacgcgca
     8461 tgttgctgaa taacggtcat caacgtattg gttatctttc ttccagtcac ggcattgaag
     8521 atgacgccat gcgtaaagca ggctggatga gtgcgttgaa agagcaggat attattccgc
     8581 cggaaagctg gattggcact ggtacgccgg acatgccggg cggtgaggcg gcgatggttg
     8641 aactgctggg gcgcaatcta caacttaccg ctgtatttgc ttataacgac aatatggccg
     8701 ctggcgcact gacagcatta aaagataatg gcattgcgat tccgttacat ctctcaatca
     8761 tcggtttcga tgatattccc atcgcccgtt acaccgaccc gcaattaacg accgtgcgtt
     8821 atcccattgc ttcaatggct aaattagcca ccgaactggc cttgcagggg gcagcaggca
     8881 atattgatcc tcgtgccagc cactgtttta tgccgacgtt agtgcgtcgc cattctgtcg
     8941 caacgcgcca gaatgcggcg gcgatcacta actcaacaaa tcaggcgatg taaccgcttt
     9001 caatctgtga gtgatttcac agtatcttaa caatgtgatg gctatgattg caccgtttta
     9061 acgttgtaac ccgtatgtaa cagtgaataa tcactttcac cgaggtaata gcgtcataac
     9121 aacaattaaa gccgttttct ggagcgttac cgggcatgga agaacgaatt ttaaaaagtg
     9181 agcttcggcg ttcagtaaca cttcattaac tctactgccc cgccgagcat ttatctcaag
     9241 cactaccctg cataagaaaa accggagata ccatgaataa gaaggtgtta accctgtctg
     9301 ctgtgatggc cagcatgtta ttcggtgccg ctgcacacgc tgctgatact cgcattggtg
     9361 taacaatcta taagtacgac gataacttta tgtctgtagt gcgcaaggct attgagcaag
     9421 atgcgaaagc cgcgccagat gttcagctgc tgatgaatga ttctcagaat gaccagtcca
     9481 agcagaacga tcagatcgac gtattgctgg cgaaaggggt gaaggcactg gcaatcaacc
     9541 tggttgaccc ggcagctgcg ggtacggtga ttgagaaagc gcgtgggcaa aacgtgccgg
     9601 tggttttctt caacaaagaa ccgtctcgta aggcgctgga tagctacgac aaagcctact
     9661 acgttggcac tgattccaaa gagtccggca ttattcaggg cgatttgatt gctaaacact
     9721 gggcggcgaa tcagggttgg gatctgaaca aagacggtca gattcaattc gtattgctga
     9781 aaggtgaacc gggccatccg gatgcagaag cacgtaccac ttacgtgatt aaagagctga
     9841 acgacaaagg cattaaaact gaacagttac agttagatac cgctatgtgg gataccgctc
     9901 aggcgaaaga taagatggac gcctggctgt ctggcccgaa cgccaacaaa atcgaagtgg
     9961 ttatcgccaa caacgatgcg atggcaatgg gcgcggtaga agcactgaaa gcacacaaca
    10021 agtccagcat tccggtgttt ggcgtcgatg cgctgccaga agcgctggcg ctggtgaaat
    10081 ccggtgcact ggcgggcacc gtactgaacg atgctaacaa ccaggcgaaa gcgacctttg
    10141 atctggcgaa aaacctggcc gatggcaaag gtgcggctga tggcaccaac tggaaaatcg
    10201 acaacaaagt ggtccgcgta ccttatgttg gcgtagataa agacaacctg gctgaattca
    10261 gcaagaaata agactgattg ggcgcagtct attactgcgc ccttattgta tgaccaacaa
    10321 ggtaaaatta tatggtcagc tcaacgactc cgtcctccgg ggaatacttg ttggaaatga
    10381 gcggtatcaa caagtccttt cctggtgtta aggcacttga taacgttaat ttaaaagtcc
    10441 ggccacattc tatccatgca ttaatggggg aaaacggtgc aggaaaatcg acattattaa
    10501 aatgcctgtt tggtatttat caaaaagact ccggcaccat tttattccag ggtaaagaga
    10561 tcgatttcca ttctgcaaaa gaagccctgg aaaatggtat ttcgatggta caccaggagt
    10621 taaacctggt attacaacgt tcggtgatgg ataacatgtg gctggggcga taccccacca
    10681 aaggcatgtt tgtcgatcag gacaaaatgt accgcgaaac caaagcgatt tttgatgaac
    10741 tggatattga tatcgatccg cgtgcgcgcg tcggcacatt atctgtttcg caaatgcaga
    10801 tgatcgaaat cgccaaagcg ttttcctata acgcgaaaat tgtgattatg gatgaaccga
    10861 cttcttcgtt aaccgaaaaa gaggtcaatc atctgttcac tattattcgt aaattaaaag
    10921 agcgcggctg cggtattgtt tatatctcgc ataaaatgga agaaatcttc cagttatgtg
    10981 atgaagttac cgtattgcgc gacggtcagt ggatcgccac tgaaccgctg gcaggactga
    11041 cgatggacaa gatcatcgcc atgatggttg ggcgttctct taaccagcgt ttccctgaca
    11101 aagaaaacaa gccgggcgaa gtcatcctcg aggtgcgtaa cctgacgtca ctgcgccagc
    11161 cgtcgattcg cgatgtctcg tttgatctgc ataaagggga gatcctcggt attgccggtc
    11221 tggtgggggc gaaacgtacc gatattgttg agacgttatt tggtattcgc gagaaatcgg
    11281 ctggcaccat aacgttgcac ggcaaacaga tcaataacca taatgccaac gaagccataa
    11341 accacggatt tgcactggta actgaggagc gccgctcaac gggaatttat gcctatctgg
    11401 atatcggttt taactcgtta atttccaata ttcgcaacta caaaaataaa gttggtttac
    11461 tggataactc gcggatgaaa agcgataccc agtgggtgat tgattcgatg cgggtgaaaa
    11521 cgccgggtca tcggacgcaa attggttcgc tctcaggtgg taatcagcaa aaggtgatta
    11581 ttggtcgctg gctgttaacg caaccagaaa tattaatgct cgatgaaccg acgcgcggta
    11641 ttgatgtcgg ggcgaagttt gaaatttatc agttaattgc cgaactggcg aagaaaggca
    11701 aggggattat tattatctcc tctgaaatgc ctgagttgtt agggataaca gaccgtattc
    11761 tggtcatgag caatggtctc gtttccggaa ttgtcgacac aaaaacaaca acgcaaaacg
    11821 aaattctgcg tcttgcgtct ttgcaccttt aagattaggg gcttcccatg agtgcgttaa
    11881 ataagaaaag ttttcttact tacctgaaag agggcggtat ttacgtcgtt cttttagttt
    11941 tgctggcgat tattattttc caggacccaa catttttaag tctgttgaac ttaagtaata
    12001 ttctcaccca gtcatcggtg cgtattatta tcgcgctcgg tgtggcaggg ttaattgtca
    12061 cccaggggac cgatctttct gctggtcgtc aggtagggct ggcggcagtg gtggctgcga
    12121 cattattgca gtccatggat aacgccaaca aagtgttccc ggaaatggcg acgatgccga
    12181 ttgcgctggt tattctgatt gtctgtgcca ttggtgcggt gatcggttta atcaacggtc
    12241 tgattatcgc ttatctcaac gtgacgccgt tcattaccac gctcggcacg atgatcatcg
    12301 tctatggcat caactcgctc tattacgact ttgtcggggc gtcgccaatt tctggttttg
    12361 acagtggctt ctctaccttt gctcagggct ttgtcgcgct ggggagtttc cgtctctctt
    12421 acatcacctt ctacgcgttg attgcggtgg cgttcgtctg ggtgttgtgg aacaaaaccc
    12481 gcttcggtaa gaacattttt gccattggcg gtaacccgga agcggcaaaa gtatctggtg
    12541 tcaacgtcgg cctgaacctg ctgatgatct acgcgttgtc tggtgtgttc tatgccttcg
    12601 gcgggatgtt agaagccgga cgtatcggct ctgccaccaa caacctcggc tttatgtatg
    12661 agctggatgc tatcgcggcg tgcgtggtag gcggtgtatc gttcagcggc ggtgtgggga
    12721 cggtgattgg cgtggtgacc ggggtaatta tttttaccgt catcaactat ggcctgacgt
    12781 atatcggcgt aaacccatac tggcagtaca tcatcaaagg ggcgattatt atcttcgccg
    12841 tagcgctgga ttcactgaaa tacgcgcgta agaagtgatg tgattgtgga tattgccgga
    12901 tgcgctgcat ccggcaatcg gtgccaaatg ccggatgcgt ctctggtttt acaacgttac
    12961 cgggtgttct ttctcgcctt tcttaaactt cacttccccg agctcaatac aacccaccgg
    13021 gcagacgtga ccacaaagca gacaacccac acatttctcg gtattacaat gcggtgtgcg
    13081 ggttttctcg ctccattcca tcgcctggtg accgccgtcg taacaggaaa tataacagcg
    13141 tccacagcca acacatttat caagattgat acggggatag acaatataac tgcggtctaa
    13201 atcttccgcc gggacaatat tgttattcgc cagacctacc atctcctgca acgaatcaaa
    13261 cccttgatcg gcgagataat gcgacaaccc gctcgccata tcttccacta tccgatagcc
    13321 gtactgcata atgccggtgg tgacctgtaa cgttgctgcg ccgagcaata aaaactccgc
    13381 cgcatcttcc caggtttcaa tgccgccgat accgctgatc gggaaatcgc gcagttctgg
    13441 atgggtgcgc atttgctgaa taaagcgcag ggcgatcggt tttaccgctt taccggaata
    13501 tccggaaata ctcgatttac cgttcacgat cggcataccg attttctgat taagatcgat
    13561 attggtgatg gatttaacgg tgttaattgc cgcaatgcca tctgcgccgc cgcgcttcgc
    13621 cgccagcgcc acttcgcaca tatcaccgat attcggtgtc atcttcgcca gcatcggcag
    13681 cgtggaaccc cgtttaactg cccgacaata tttttctacc agctccgggc tttgcccgac
    13741 atcgctaccc atcgcatgag acgtcatttg cggacaggag aagttacact cgatcatatc
    13801 cgcgccagct tcttgcacca ggcgcgccag ctcctcccat tgctgctcat tttcccccat
    13861 gatcgaagcg atcaatactt tgtccgggta atcttccttc agccgacgca gggcggccag
    13921 attctcttcc aacggatgct cggcaatctg ctccatattt ttgaagccga taaaaccggt
    13981 atcttctttc accagatgat caaaacgcgg cgagacttcg ttggcgataa aaaagccgat
    14041 cgttttaaac accacgccgc cccaacctgt gtcgtaggct ttggcacaca tctcatagca
    14101 gttgcctacc ggcgaagaag agaggcagaa cgggttggga aacttcacgc cgcaaaaggt
    14161 aatcgaaaga tctttcgtta acatgagcaa gctccctcta aatagtgatg aatcgcctcg
    14221 gcggcttctt tcccggtttt cacggcatag accacggttt tgtcaccctc aacaatatcg
    14281 ccagcagcaa agacttgcgg atcgcgggtc tgataatttt gtgttttgat ggtgttacgc
    14341 tgcggctcta acttcgcaaa ggcatccagt ctggcatgct gaccgacggc gagaataatt
    14401 ttatctgcca ccatcgtcag ttcgcccggt aaccgtacat gcttaaacgt gactttattg
    14461 ccttccacgg ctactggcgt gaatccatcg atgatcgaaa cgcccagctc ccgggcgctg
    14521 gtaaactctt tttcgcttgc cggaaactca tctaactctt cacgcgctac gcaagttacc
    14581 gcctgacagc cgagaacttt cagcgtgctg gctacgtcca tcgcgacatc accgccgccg
    14641 ataattaatg cgctttgtgg aatgctgata tcgccttgcg cctgtcgtgc acgttgcaag
    14701 aagtcgacgg caatctcaac gtcgctatgc tcaaacagcg gtagcccgga accgcttgat
    14761 aaccccacgg tgaccagtac cgcgcggttt tccgctttca gttgctccag ggtgagtgtg
    14821 ttaccgactt cgttgttgca cttaatggtc acgcccattt tttcaatacg ggcgatctct
    14881 gcatccagca ctgattgcgg taaccggaat tgcggaatac cgttacgcaa ccagccaccg
    14941 gggtgcgctt ctttctcata aatcgtgacg tcataaccct ggtttgtcag tgtcacactg
    15001 gcctgcaatc cggcaggacc tgcgccaata atcgcgactt tgccgagcgt tttagtaccg
    15061 ggctgataaa tttccattcc ggtttgttgt tcaaaatcag taacaaaacg ctgtaagcgg
    15121 ccgatatcaa tgggagcatc aacaccggca cgggtacaac cgctttggca taatttctcc
    15181 gtcgggcaca ctctggcaca aacggcaccg agggcattat tttcgcgaat tgtctcggca
    15241 gcgcctttaa aattacgaaa gtagattgag cgaataaatt tccccggatc ggtctgtgcc
    15301 gggcaagcct gactacaggg agcgtcgtga cataataaac agcgagaggc ttctttaatc
    15361 gccagtaaag acgtaaaagc cggagtgagt tcatccagat aattttgttg cggcatgtga
    15421 tcttccttaa tgctataggt aattaaggcg agcgtttagt aaataataat gtactgatat
    15481 taaggctaag cgtgcggggg aatttaaact gt
//
LOCUS       DAPHBJ010000101        15323 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.102, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000101 DAPHBJ010000000
VERSION     DAPHBJ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.102"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1157
                     /locus_tag="RHZ76_002801"
     CDS             <1..1157
                     /locus_tag="RHZ76_002801"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDV2813412.1"
                     /translation="NWIDKEEYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGS
                     SEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRP
                     GQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            1313..2848
                     /gene="gadC"
                     /locus_tag="RHZ76_002802"
     CDS             1313..2848
                     /gene="gadC"
                     /locus_tag="RHZ76_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HDV2813413.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2979..4298
                     /locus_tag="RHZ76_002803"
     CDS             2979..4298
                     /locus_tag="RHZ76_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HDV2813414.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4670..6052
                     /gene="dosC"
                     /locus_tag="RHZ76_002804"
     CDS             4670..6052
                     /gene="dosC"
                     /locus_tag="RHZ76_002804"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HDV2813415.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSNYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            6077..8464
                     /gene="dosP"
                     /locus_tag="RHZ76_002805"
                     /gene_synonym="pdeO"
     CDS             6077..8464
                     /gene="dosP"
                     /locus_tag="RHZ76_002805"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HDV2813416.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEIQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLFLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFKRIQI
                     LRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAITSIG
                     QSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLKI"
     gene            8722..9303
                     /gene="ddpX"
                     /locus_tag="RHZ76_002806"
     CDS             8722..9303
                     /gene="ddpX"
                     /locus_tag="RHZ76_002806"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HDV2813417.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLTDQFSCFISPGTQHVS"
     gene            9317..10867
                     /locus_tag="RHZ76_002807"
     CDS             9317..10867
                     /locus_tag="RHZ76_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV2813418.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGAFIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10869..11891
                     /locus_tag="RHZ76_002808"
     CDS             10869..11891
                     /locus_tag="RHZ76_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2813419.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGKLSAVWRNRWLDHLVRIMVITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWCIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11888..12784
                     /locus_tag="RHZ76_002809"
     CDS             11888..12784
                     /locus_tag="RHZ76_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HDV2813420.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12781..13767
                     /locus_tag="RHZ76_002810"
     CDS             12781..13767
                     /locus_tag="RHZ76_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2813421.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIDLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGIPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            13760..14686
                     /locus_tag="RHZ76_002811"
     CDS             13760..14686
                     /locus_tag="RHZ76_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2813422.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(14742..15173)
                     /gene="osmC"
                     /locus_tag="RHZ76_002812"
     CDS             complement(14742..15173)
                     /gene="osmC"
                     /locus_tag="RHZ76_002812"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HDV2813423.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         3724 a         3832 c         3932 g         3835 t
ORIGIN      
        1 aaaactggat cgacaaagaa gaatatccgc aatccgcagc catcgacctg cgttgcgtaa
       61 atatggttgc cgatctgtgg catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca
      121 acaccattgg ttcttccgag gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc
      181 gcaagcgtat ggaagctgca ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg
      241 tgcaaatctg ctggcataaa ttcgcccgct actgggatgt ggagctgcgt gagatcccta
      301 tgcgccccgg tcagttgttt atggatccga aacggatgat tgaagcctgc gacgaaaata
      361 ccatcggcgt ggtgccgact ttcggtgtga cctacaccgg taactatgag ttcccgcaac
      421 cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc gacatgcaca
      481 tcgacgccgc cagtggtggc ttcctggcac cgttcgtcgc cccggatatc gtctgggact
      541 tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt ctggctccgc
      601 tgggctgcgg ctgggttatc tggcgtgatg aagaagcgct gccgcaggaa ctggtgttca
      661 acgttgacta cctggggggt cagattggta ctttcgccat caacttctcc cgcccggcag
      721 gtcaggtgat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc tataccaaag
      781 tacagaacgc ttcctaccag gtcgctgcct atctggcgga tgaaatcgcc aaactggggc
      841 cgtatgagtt catctgtact ggtcgcccgg acgaaggcat cccggcggtt tgcttcaaac
      901 tgaaagatgg tgaagatccg ggatacaccc tgtatgacct ctctgaacgt ctgcgtctgc
      961 gcggctggca ggttccagcc ttcactctcg gcggtgaagc caccgacatc gtggtgatgc
     1021 gcattatgtg tcgtcgcggc ttcgaaatgg actttgctga actgttgctg gaagactaca
     1081 aagcctccct gaaatatctc agcgatcacc cgaaactgca gggtattgcc cagcagaaca
     1141 gctttaaaca tacctgataa cgtttaacgg taacggtgtc ccgaaacgca cccgtttcgg
     1201 gacaatttcc aaagtctgtt cactggcatt agcaacggaa aatattgttc tgaatacgct
     1261 tcagaacaaa acaggtgcgg ttccgacagg aataccgttt tagggggata atatggctac
     1321 atcagtacag acaggtaaag ctaagcagct cacattactc ggattctttg ccataacggc
     1381 atcgatggta atggctgttt atgaataccc taccttcgca acatcgggct tttcattagt
     1441 cttcttcctg ctattaggcg ggattttatg gtttattccc gtgggacttt gtgctgcgga
     1501 aatggccacc gtcgacggct gggaagaagg tggtgtcttc gcctgggtat caaatactct
     1561 ggggccgaga tggggatttg cagcgatctc atttggctat ctgcaaatcg ccattggttt
     1621 tattccgatg ctctatttcg tgttaggggc actctcctac attctgaaat ggccagcgct
     1681 gaatgaagac cccattacca aaactattgc agcactcatc attctttggg cgctggcatt
     1741 aacgcagttt ggtggcacga aatacacggc gcgaattgct aaagttggct tcttcgccgg
     1801 tatcctgtta cctgcattta ttttgattgc attagcggct atttatctgc actccggtgc
     1861 ccccgttgct atcgaaatgg attcgaagac cttcttccct gacttctcta aagtgggcac
     1921 cctggttgta tttgttgcct tcattttgag ttatatgggc gtagaagctt ccgcaaccca
     1981 cgtcaatgaa atgagtaacc ccgggcgcga ctatccactg gctatgttac tgctgatggt
     2041 ggcggcaatc tgcttaagct ctgttggcgg tttgtctatt gcgatggtca ttccgggtaa
     2101 tgaaatcaac ctctccgcag gggtaatgca aacctttacc gttctgatgt cccatgtggc
     2161 accggaaatt gagtggacgg ttcgcgtgat ctccgcactg ctgttgctgg gtgttctggc
     2221 ggaaatcgcc tcctggattg ttggtccttc tcgcgggatg tatgtcacag cgcagaaaaa
     2281 cctgctgcca gcggcattcg ctaaaatgaa caaaaatggc gtaccggtaa cgctggtcat
     2341 ttcgcagctg gtgattactt ctatcgcgtt gatcatcctc accaataccg gtggcggtaa
     2401 caacatgtcc ttcctgatcg cactggcgct gacggtggtg atttatctgt gtgcttattt
     2461 catgctgttt attggctaca ttgtgttggt tcttaaacat cctgacttaa aacgcacatt
     2521 taatatccct ggtggtaaag gggtgaaatt ggtcgtggca attgtcggtc tgctgacttc
     2581 aattatggcg tttattgttt ccttcctgcc gccggataac atccagggtg attctaccga
     2641 tatgtatgtt gaattactgg ttgttagttt cctggtggta cttgccctgc cctttattct
     2701 ctatgctgtt catgatcgta aaggcaaagc gaataccggc gtcactctgg agccaatcaa
     2761 cagtcagaac gcaccaaaag gtcacttctt cctgcacccg cgtgcacgtt caccacacta
     2821 tattgtgatg aatgacaaga aacactaacc atgtctgaat ggttataaga caagggagcg
     2881 ataagtcatc gctccctttt tcgtgcttgc tgtcgtattg acttaaccgg gataagtacg
     2941 agaatgagcg caaatctgtt taccggaaac cagcacatat ggatatctgc tcccgaaaca
     3001 agaaattaac gattagaaga ccagcgatac tagttgcact ggcactttta ctgtgtagtt
     3061 gtaaaagcac gcctccagag tccatggtga caccaccagc aggttcaaag ccaccagcca
     3121 cgacgcaaca atcgtcacaa ccgatgcgtg gcatctggct ggccacggtt tctcggctcg
     3181 actggccacc ggtttcctcg gttaacatta gtaaccccac cagccgggcc cgtgtacaac
     3241 aacaggcgat gatcgacaaa ctggatcatc tgcaacgtct cggcataaac acggtctttt
     3301 tccaggtcaa gccggacggt accgccctgt ggccatcgaa aattttgccg tggtccgatc
     3361 ttatgactgg taagattggt gaaaatccgg gttacgatcc gctgcaattt atgctcgacg
     3421 aagcccacaa gcgtgggatg aaagtacacg cctggtttaa cccctatcgc gtatcggtta
     3481 atacgaagcc cggtactatc agggaactga atagcactct gtctcaacaa ccggcgagcg
     3541 tctatgtgca acaccgcgac tggatcagaa cgtctggcga tcgctttgtc ctcgacccgg
     3601 gcatccctga ggttcaggac tggatcacat caatagtcgc agaagtggtt tcccgctatc
     3661 ccgtagatgg cgtgcagttt gacgactatt tctatacgga gtcaccgggt tcacggctaa
     3721 atgataacga aacgtaccgt aaatacggag gcgcatttgc gtcaaaagca gactggcggc
     3781 gcaacaatac tcagcagtta attgcaaagg tatcgcacac cattaaaagc attaagccgg
     3841 gagtcgaatt tggtgttagc ccggcaggcg tgtggcgtaa ccgatcacac gatccgctcg
     3901 gttccgatac ccgaggcgcg gcagcctatg acgaatccta cgctgacacc cgtcgatggg
     3961 tggaacaagg attgctggat tacattgctc cccaaattta ctggccgttc tcacggagtg
     4021 ccgcgcgtta tgacgtgttg gcaaaatggt gggcggatgt cgttaaaccg accaggaccc
     4081 gcctgtatat cggtatcgcc ttctataaag tgggtgaacc ttcaaagata gagccagact
     4141 ggatgattaa cggcggcgta ccggaactga aaaagcagct cgatcttaac gatgcggtac
     4201 cagaaattag cggcaccatc ttgttccgtg aggactatct gaataaaccg cagactcaac
     4261 aagcggtcag ctatctgcaa agtcgttggg gcagttaaac tcccatgctt actttgtaac
     4321 aagccggtgc tttacccacc ggcttgttac accttgtgaa atatcccact agtaatcatg
     4381 cttacataag tcaaattaac cacgcaatta accacactta gttaaacagc tataaatccc
     4441 taataaaaat gtatgaatat gaaaaaccat acgatattcg ctgtcaccga aaaataatta
     4501 cgatgggcta tactcttctg aagaacagta gcgtagattg aaactgtcct aagtcgctta
     4561 tttcttttca gtatatcttc atatttcagg agaatatggt gcagttagtc gatctcgcac
     4621 gttgcgtttt gattccgtct gatttcccag ccttataagg gtgtgaatta tggagatgta
     4681 ttttaaaaga atgaaagatg agtggaccgg acttgtcgaa caggcagatc cgcccattcg
     4741 tgctaaagcc gcggaaattg ccgttgcgca tgctcattat ctgagtattg agttttatcg
     4801 aattgtccgc atcgacccgc atgccgaaga attcttgagt aatgaacaag ttgagcggca
     4861 gttgaaaagt gcgatggaac gctggattat taacgtgctt tctgcccagg ttgacgatgt
     4921 cgaaaggcta atacaaatcc agcataccgt cgcggaagtg catgcccgca taggaattcc
     4981 ggtagaaatt gtcgagatgg ggtttcgggt gctgaaaaaa atcctctatc cggtaatctt
     5041 ctcttcgaat tattccgccg cagaaaaact tcaggtctac catttttcga ttaacagtat
     5101 tgatatcgct atggaagtga tgacccgcgc gtttaccttt agtgacagta gtgcctcaaa
     5161 agaagatgaa aactatcgta tcttctcgtt actggaaaac gccgaagaag aaaaagaacg
     5221 gcaaatagcc tcaatacttt catgggaaat agatattatc tataaagtcc tgctggattc
     5281 tgatttaggc agtagtttgc ctttaagcca ggctgatttt ggcctgtggt ttaaccataa
     5341 aggtcgacat tatttcagcg gcattgctga agtgggccat atctctcgtc tgattcagga
     5401 tttcgacggt attttcaatc aaaccatgcg taacaccagg aatttgaata acagaagtct
     5461 gcgggtgaaa tttttattac agataagaaa taccgtatcg caaattatta ccttgctgcg
     5521 tgaattgttt gaagaagtat cgcgccacga agtcggtatg gatgtactga cgaaattact
     5581 taaccgccgt ttcctgccga ctatcttcaa acgcgaaatt gcccatgcca accggaccgg
     5641 tacaccgctg tcagtgctga ttattgacgt tgataaattc aaagagatca acgatacgtg
     5701 gggccataac actggcgatg aaattctgcg taaagtctct caggcctttt atgacaacgt
     5761 ccgcagtagt gattatgttt tccgctacgg gggcgatgaa tttatcattg ttttgactga
     5821 agcttctgaa aacgaaacgt tacgtaccgc agaacgtatt cgcagtcggg tggagaaaac
     5881 caaactgaaa gccgcaaacg gcgaggatat tgccctctca ctttccatcg gtgccgccat
     5941 gtttaatggt catcctgact atgagcgcct cattcaaata gccgatgaag ctctgtatat
     6001 cgccaaaaga cgaggtagaa accgtgttga actctggaaa gccagtcttt agatgcgcca
     6061 ggatgcagag gtaatcatga agctaaccga tgcggaaaat gccgccgatg gcattttttt
     6121 ccccgccctt gaacaaaata tgatgggcgc ggtgttaatt aacgaaaatg atgaagtgat
     6181 gtttttcaac cccgccgcag agaagctctg ggggtacaaa cgtgaagaag tcattggcaa
     6241 taacattgat atgctgattc cgcgggattt gcgtcctgcg catcctgaat acattcgtca
     6301 taaccgtgaa ggtggtaaag cgcgcgtcga ggggatgagt cgggagctgc agctggagaa
     6361 aaaagacggc agtaaaatct ggacccgttt tgcgctatcg aaagtgagcg ccgaggggaa
     6421 agtttattac ctggcgctgg tacgggatgc cagcgtagaa atggcgcaaa aagaacagac
     6481 ccgacaattg attattgccg ttgaccatct cgaccgaccg gtgattgtcc tcgatccgga
     6541 acgccatatt gtgcagtgca atcgcgcatt taccgaaatg tttggttact gcattagcga
     6601 agccagcggt atgcagcccg atacactcct gaacattcct gaattccctg ccgataaccg
     6661 cattcgttta caacagttgc tatggaaaac cgcccgcgat caggacgaat ttctgctgtt
     6721 gacgcgcacc ggtgaaaaaa tctggattaa agcctctatc agcccggttt atgacgtgct
     6781 cgcgcatctg cagaacctgg taatgacttt ctcggatatc accgaagaac ggcaaatccg
     6841 ccagcttgaa ggcaatattc tcgccgccat gtgcagcagc ccgccatttc atgaaatggg
     6901 ggaaatcatt tgtcgtaaca tcgaatctgt actcaacgaa tcgcatgttt cgctgttcgc
     6961 actgcgcaac gggatgccga tacactgggc gtcatcttcc cacggtgcag aaattcaaaa
     7021 tgcgcaaagc tggtcagcga ccattcgtca gcgtgatggc gcgcctgcgg ggatcctgca
     7081 aattaaaacc tcgtcaggag cagaaaccag cgcctttatc gaacgcgtgg cagatatcag
     7141 ccagcatatg gccgcgctgg cgctggaaca ggaaaaaagc cgtcagcata ttgaacaact
     7201 catccaattt gatccgatga ccggtctgcc aaatcgcaat aacctgcaca attacctcga
     7261 tgacctggtc gacaaagccg tctctcccgt ggtgtatctc atcggtgttg accatattca
     7321 ggatgtgatt gatagccttg gctatgcgtg ggccgatcag gcattgctgg aagtggtcaa
     7381 tcgctttcgt gaaaaactca aaccggatca gtatctctgt cgtatcgaag gtacgcagtt
     7441 tgtcctcgtg agcctcgaaa acgacgtcag taacattacc caaatcgccg atgagctacg
     7501 gaatgtggtc agcaagccga taatgattga cgataaaccc ttcccgctta ccttgagtat
     7561 tggcatcagc tacgacgtgg gtaaaaaccg cgattacttg ctctccactg ctcacaatgc
     7621 aatggattat attcgcaaga atggcggtaa cggctggcag ttcttcagcc cggcgatgaa
     7681 cgaaatggta aaagagcgtt tgtttttagg cgcagcgctg aaagaagcga ttagcaataa
     7741 ccaactgaaa ctggtttacc agccgcaaat cttcgcagaa acgggtgaac tgtacggcat
     7801 cgaagccctt gctcgctggc acgatcccct gcatggtcat gtgccccctt cacggtttat
     7861 tcctctcgca gaagagattg gtgaaatcga aaatattggg cgctgggtca tcgcggaagc
     7921 ttgccgtcag ttagcagaat ggcgtagcca gaatattcat atcccggcgc tttccgttaa
     7981 cttgtcggca ctgcactttc gcagtaatca ggtgtctgat gcaatgcaag cctggggtat
     8041 tgacggccac cagctgacgg tggaaatcac ggaaagcatg atgatggaac acgataccga
     8101 aatctttaag cgcattcaga tcctgcgcga tatgggcgtg ggcttatcgg tagatgattt
     8161 tggcacgggc ttttccggat tatcccgctt agtcagtctt ccggtaacgg aaatcaaaat
     8221 tgacaaaagt tttgtcgatc gttgtctgac ggaaaaacgc atccttgcct tacttgaagc
     8281 cattaccagc attgggcaaa gcctcaattt aaccgtcgtg gcggaaggcg tcgaaaccaa
     8341 agagcaattt gagatgctac gcaagatcca ctgtcgcgtt attcagggat atttcttttc
     8401 ccgcccccta cccgccgaag aaattccagg ctggatgagc agcgtgttac cgctgaaaat
     8461 ctgacaaatt cctctcgccc gcactcgcgg gttttcattt aacgtgacac tgtcacttaa
     8521 atactgtgat ttcagctacg attccgggag attccttcct taacttcccc tcttgcacca
     8581 aaactgcact acgctgcaca aatgttgatc aaaagttaaa aaatataact ttaagccagc
     8641 attctggcgc ttatcccggc atggcatgag atctgcataa gcggaaagcg cagcaatttt
     8701 tgtcttatac aggaaccgtt tatgtcggat accaccgaac tggttgattt agccgtgatc
     8761 ttccctgatc tggagatcga attgaaatac gcctgcgctg ataacatcac aggtaaagct
     8821 atttaccagc aagcgcgttg tctgttacac aaggatgcga ttaccgcgct ggcgaaaagt
     8881 atcagtatcg cccagctctc aggcttacaa ctggtgattt acgacgcgta tcgcccacaa
     8941 caagcacagg cgatgttgtg gcaagcctgc ccagacccgc aatatgttgt tgatgtgacg
     9001 gtcggttcta atcacagccg tggcacggcg atcgacctga cgcttcgtga tgagcacggg
     9061 aacatcctcg atatgggcgc aggattcgat gaaatgcacg aacgttccca tgcttaccat
     9121 ccttccgtcc cgcccgccgc tcagcgcaat cggctgttgc tgaatgcgat tatgactggt
     9181 ggcggttttg tcggtatctc cagcgaatgg tggcacttcg aattacccca ggcagcgagt
     9241 tacccactgc tcactgatca attctcttgt tttatatccc ctggcacaca gcacgtcagt
     9301 taatcaggaa actgtcatga agagatcgat atcgtttcgt cccacattgc tcgcgctcgt
     9361 ccttgccaca actttcccgg ttgcgcacgc cgccgtaccg aaagatatgc tggtgattgg
     9421 taaggccgcc gatccacaaa ccctcgaccc ggcggtaaca atagataata acgactggac
     9481 agtgacctac ccgtcttatc agcggctggt tcagtacaaa acggacggtg ataaaggctc
     9541 aaccgacgtt gaaggcgatc tggcaagtag ctggaaagcg tctgacgatc aaaaagagtg
     9601 gacgttcacc ctgaaagata atgctaaatt tgccgatggc acacctgtca ctgccgaagc
     9661 agtaaaactc tcttttgagc ggttactaaa aatcggccag gggccagcag aagcatttcc
     9721 caaagattta aagattgata ctcccgacga acatacggtg aagtttaccc ttagccaacc
     9781 attcgcaccg ttcctctaca cgctggcgaa tgacggtgca ttcattatca atccggcggt
     9841 gttaaaggaa catgcagcgg atgatgctcg cggcttcctc gcgcaaaata ccgccggttc
     9901 cggaccattt atgctgaaaa gctggcaaaa aggtcagcaa ttagttctgg tgccaaatcc
     9961 gcattacccc ggcaataaac cgaactttaa gcgagtatcg gtaaaaatta tcggtgaaag
    10021 tgcctcccgt cgcctgcagc tctcccgtgg cgacattgac attgccgatg cgctgccggt
    10081 ggatcaactc aacgccctga agcaggaaaa taaagtcaat gtggcagagt atccgtcact
    10141 gcgcgtcacc tatctgtatc tgaataacag caaagcgcca cttaatcagg cggatctgcg
    10201 tcgggccatt tcctggtcta ccgattatca gggcatggtt aacggcattc tgagtggtaa
    10261 cggaaaacag atgcgcggcc cgattccgga aggcatgtgg ggctacgatg cgacggcaat
    10321 gcaatacaac catgacgaaa cgaaagccaa agccgaatgg gataaagtga cgagcaaacc
    10381 caccagcctg acgtttctct actccgataa cgatccgaac tgggagccta ttgctctggc
    10441 gacacaatcc agtctcaaca agctgggcat caatgtgaag ctggaaaagc tggcgaacgc
    10501 caccatgcgc gacagagtgg gtaaaggtga ttacgacatt gcgattggca actggagtcc
    10561 ggattttgcc gacccgtata tgtttatgaa ttactggttt gagtcagaca aaaaaggtct
    10621 gccgggtaac cgctcgttct atgaaaacag tgaggtcgat aagttactgc gcaatgcgct
    10681 tgcgaccacc gaccagacgc agcgtacccg ggactaccag caggcacaga aaatcgtcat
    10741 tgatgacgct gcttatgtgt acctgttcca gaaaaactac caactggcga tgaacaaaga
    10801 ggtgaaaggc tttgtgttca atcccatgct ggaacaggtc ttcaatatca ataccatgag
    10861 taaataacgt gaccttctgg agtattttac gccaacgctg ctgggggctg gtgctcgtgg
    10921 tggcgggcgt ctgcgtgatt acgtttatta tctcgcatct gatccctggc gatccggcgc
    10981 ggttactggc gggtgaccgc gccagcgatg ctatcgtgga aaatattcgc cagcaactgg
    11041 gactggacca gccactgtat gtacagtttt accgctacgt cagcgacctg tttcatggtg
    11101 acctgggaac atccattcgt accgggcgtc cggtgctgga agagttgcgt atatttttcc
    11161 cggcgacgct ggaactggct ttttgtgccc tgctgctggc actcctgatt ggcatcccgc
    11221 tgggcaaact ctctgcagtc tggcgaaatc gctggctgga tcatctggtg cgaataatgg
    11281 tcattaccgg aatctccaca cctgcgttct ggcttggact gggcgtcatt gtgctgtttt
    11341 atggtcatct gcaaattctt cccggcggcg gaaggcttga tgactggctg gatccaccaa
    11401 cgcacgttac cggcttttat ctgctcgatg cgctgcttga aggcaacggt gaagtcttct
    11461 tcaatgcgtt gcaacatctc atcttaccgg cattaacgct ggcgttcgtt cacctgggaa
    11521 ttgtcgctcg ccagatccgc tcagcgatgc tggaacaatt gagtgaagac tacattcgta
    11581 ccgcccgggc cagcggcttg cccggctggt gtatcgtttt atgttatgcg ctacccaatg
    11641 cgttgatccc atcgattacc gtattgggtc tggcgctggg cgatttgttg tatggcgcag
    11701 tgctcaccga aaccgttttt gcctggcccg gaatgggtgc atgggtagta acatcaatac
    11761 aggcgctcga cttcccggca gtgatgggct ttgccgtcgt ggtttcattt gcttatgtgc
    11821 tggtcaacct ggtggtggat ttgctctatt tgtggattga tccgcgtatc ggacgtggag
    11881 gtggtgaatg atgctaagcg aggaaacgtc cgccgtacgc ccacaaaaac aaacgcgatt
    11941 taacggtgca aaactggtat ggatgctgaa aggcagtccg ctcaccgtga ccggcgcagt
    12001 catcattgta ttaatgctac tgatgatgat tttttcaccg tggctggcga cgcatgatcc
    12061 caacgccatt gatttaaccg cccgcctttt gccgccttct gcggcgcact ggtttggcac
    12121 cgatgaagtg ggacgcgatc tgttcagccg cgtactggtc ggcagtcagc aatcaattct
    12181 cgccggatta gtggtggtcg ccattgcggg tatgattggt tcgctactcg gatgtctatc
    12241 cggtgtgctt ggcggacgcg cagacgccat catcatgcgc atcatggaca ttatgctgtc
    12301 gattccttcg ttggtactga caatggcact ggctgccgct ctcgggccga gtttgtttaa
    12361 cgccatgctg gctattgcta ttgtgcgaat tcccttttat gtgcgcctgg cgcgagggca
    12421 agcattagtt gtacgccagt atacctatgt tcaggcggcg aaaacctttg gtgcgtcgcg
    12481 ttggcatctg atcagctggc atattttacg taactccctg ccgccgctga tcgtgcaggc
    12541 atcgctggat atcggtagcg cgattttaat ggccgccacg ttgggattta ttggcctggg
    12601 tgctcaacaa ccgagtgctg aatggggcgc gatggtagcg aatggtcgca actatgtact
    12661 cgatcagtgg tggtattgcg catttccggg ggcagcgatt ttgcttaccg ccgttgggtt
    12721 taatctcttt ggtgatggta ttcgcgatct gcttgacccg aaagcaggag gaaagcagtc
    12781 atgacccaac ccgttctgga cattcaacaa ctgcatttga gtttccccgg ttttaacggt
    12841 gatgttcacg cgctcaacaa tgtgtccttg cagattaacc gcggtgaaat tgtcggtctg
    12901 gtgggagaat ccggctcagg taaatcagtc accgcaatgc tgattatgcg tctgctaccg
    12961 acgggcagtt attgcgtaca tcgggggcag atttccctgc tgggagaaga tgttcttaac
    13021 gcgcgggaaa aacagcttcg tcagtggcgc ggcgcacgag tggcgatgat ctttcaggaa
    13081 ccgatgaccg ccctcaatcc gacacgtcga ataggtcttc agatgatgga cgtgatccgc
    13141 catcatcaac caataagtcg tcgggaagcc agagctaaag cgattgacct gctggaagag
    13201 atgcaaatcc cggatgccgt ggaagttatg tcgcgctatc cgtttgagct ttcaggtggt
    13261 atgcgccagc gggtaatgat tgcgctggca ttctcctgcg agccgcaatt gattattgcc
    13321 gacgaaccga ctacggcgct ggacgtcacg gtacagttgc aggtactgcg tttgcttaaa
    13381 cataaagctc gagccagtgg aactgcggtg ctgttcatca gccatgatat ggccgtggtg
    13441 tcgcaactgt gcgatagcgt ttacgtgatg tatgccggaa gcgtgattga gagcggcgtg
    13501 acggcagacg ttatccatca tccccggcat ccgtatacca ttggtttgct gcaatgcgca
    13561 ccagaacatg gaataccgcg ccagccatta cccgccattc cagggacggt accaaatctc
    13621 acccatttgc ctgacggctg cgcttttcgc gatcgttgtt atgcggcagg tgcacagtgt
    13681 gaaaacgtcc cggcgctgac agcgtgtggt gacaacaacc agcgctgcgc ctgttggtat
    13741 ccgcagcagg aggtcataag tgtctgacac gttattaacg ttacgcgacg tccatatcaa
    13801 tttcccggcc cgtaaaaact ggcttggtaa aactacggaa catgttcatg cgattaatgg
    13861 tattgattta cagatccgcc gtggtgaaac cttagggatc gtcggcgagt caggctgcgg
    13921 caaaagcacc ctcgcacagc ttttaatggg tatgctgcaa ccgagccacg ggcagtacat
    13981 ccgttctggc tcacaacgca ttatgcagat ggtgtttcag gacccgctct cttcgctcaa
    14041 tccgcgctta ccggtgtggc gcatcatcac agaaccgctc tggatagcta agcgtagtag
    14101 tgaacaacag cggcgagcgt tggcagagga gctggctgtg caggtgggta ttcgtccgga
    14161 gtatctcgac cgcctgcctc atgcgttctc cggcgggcag cggcaacgta tcgccattgc
    14221 cagagcactc tcttcgcagc ctgacgtgat tgtgcttgat gagccaacct ctgcgctgga
    14281 tatctccgtg caggcgcaga tcctcaattt actggtaacg ctacaggaaa atcacgggct
    14341 gacctatgtg ctgatttcac acaatgtctc ggtgatacgt catatgagcg atcgggtggc
    14401 ggtgatgtat ctcgggcaga ttgtagaact gggcgacgcg cagcaggtgc tgacggcacc
    14461 tgcacatcca tacacccgat tattgctgga ttccctcccc gccattgata aaccgctgga
    14521 ggaagaatgg gcattacgta aaacggatct gccgggaaac cgcacgttgc cgcaaggctg
    14581 ttttttctac gaacgttgcc cgctggcaac ccacggatgt gaagtccggc aatcattagc
    14641 aataagggag gacggacgtg agctccggtg ctggcgggcg ctgtagagat gctaacaacg
    14701 catcaggcat tcacgcgcct gacgcgtcat ccggcaatgc tttacgattt caactggtaa
    14761 tccagcgtaa tttccgcttt cagtacctga gagaccgggc atcctgcttt tgctttctgg
    14821 atgatgccgt caaaggtaga ggcatcaata cccggcaccg caacttcact cttcagtgcg
    14881 attttcgtaa tcgcaaaacc ggcatccact ttatccagcg acacatcggc ggtggtatca
    14941 atcgatgttg gcgtgaatcc cgcttccccc agcattaatg aaagcgccat tgagaaacat
    15001 gcggcatgcg ctgcgccaat cagttcttca gggttggttc ctttttcgcc ttcaaaacgc
    15061 gtgttaaatc catacggctg ttggttcagc acgccactct cggtggatac tgttcccttc
    15121 ccgcgtttga tatcgccttc ccagtgtgcc tgacctttct tatggattgt cattgttgct
    15181 ctcctgtggg cttaaaaagg aaattataga ctacgtgaga aacagcacga ggataagcag
    15241 gatattccga ataaaatccg gcgaaaatat tacttctgca cttaaattaa attcccacaa
    15301 tagattaccg ggaatatata acc
//
LOCUS       DAPHBJ010000102        14948 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.103, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000102 DAPHBJ010000000
VERSION     DAPHBJ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.103"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(42..2321)
                     /gene="maeB"
                     /locus_tag="RHZ76_002813"
     CDS             complement(42..2321)
                     /gene="maeB"
                     /locus_tag="RHZ76_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HDV2813424.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSSLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2610..3560
                     /gene="tal"
                     /locus_tag="RHZ76_002814"
     CDS             2610..3560
                     /gene="tal"
                     /locus_tag="RHZ76_002814"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDV2813425.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3580..5583
                     /gene="tkt"
                     /locus_tag="RHZ76_002815"
     CDS             3580..5583
                     /gene="tkt"
                     /locus_tag="RHZ76_002815"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDV2813426.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5659..6702)
                     /locus_tag="RHZ76_002816"
     CDS             complement(5659..6702)
                     /locus_tag="RHZ76_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HDV2813427.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPEASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGDQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6828..7403)
                     /gene="nudK"
                     /locus_tag="RHZ76_002817"
     CDS             complement(6828..7403)
                     /gene="nudK"
                     /locus_tag="RHZ76_002817"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HDV2813428.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNTKKKTVVLIRQFRVATWVNGNESGQLIESCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(7471..9450)
                     /gene="aegA"
                     /locus_tag="RHZ76_002818"
     CDS             complement(7471..9450)
                     /gene="aegA"
                     /locus_tag="RHZ76_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HDV2813429.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVGVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9656..11356
                     /gene="narQ"
                     /locus_tag="RHZ76_002819"
     CDS             9656..11356
                     /gene="narQ"
                     /locus_tag="RHZ76_002819"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HDV2813430.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTNLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11520..14633
                     /gene="acrD"
                     /locus_tag="RHZ76_002820"
     CDS             11520..14633
                     /gene="acrD"
                     /locus_tag="RHZ76_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HDV2813431.1"
                     /translation="MANFFIDRPIFACVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPKLTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNSFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14732..14791)
                     /gene="ypfM"
                     /locus_tag="RHZ76_002821"
     CDS             complement(14732..14791)
                     /gene="ypfM"
                     /locus_tag="RHZ76_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HDV2813432.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3495 a         3974 c         3910 g         3569 t
ORIGIN      
        1 ttaccctgaa tattcagggt aagcgtgaga gttaaaaaaa attacagcgg ttgggtttgc
       61 gcttctacca cggccagcgc caccatgttg acgatacgac gcaccgatgc gatcggcgtt
      121 aacacgtgaa ccggtttcgc cacacccatc agcaccgggc cgacagtcac accttccgag
      181 ctggaaacac gcagtaagtt gtaactaatg cgggcagctt ccatgttcgg catcaccaga
      241 atattggcgg aacctttcaa agagctgtcc ggcatacggt cgttgcgaat cgcttccacc
      301 agcgctgcat cgccgtgcat ttcaccatca atcatcagtt ctggtgcacg ttccctgacc
      361 agttccagcg cctgacgcat tttgctcgac gacgggcagt cagaagaacc aaagttggag
      421 tgcgacaaca aagcaacgcg cggctcaata ccaaaacgac ggacagtttc tgccgccatc
      481 aaggtgatct ccgccagctc ttctgcatcc ggttcatcat taacatatgt atcggcaata
      541 aaggtgttac cactcggcag cagcagcgcg ttcatggcac ctgcggtgtg aacgccatcg
      601 cgataaccaa agacattttt caccacgcta aaatgttcat gataatcacc caccgtaccg
      661 caaatcattg catcggcttc cccacgctga accatgatcg cgccgatcac tgtcgggtta
      721 ctgatcagcg cccgctgcgc ctgttcctga gtgacgccgc gacgcttcat gatctggaag
      781 tattcggtcc agtactcttt aaagcgcgga tcggattcgt tattgacgat ctcaaaatca
      841 acgcccgctt tgatctgcaa gcccagtttc tgaatgcgca tttcgatcac gttcggacga
      901 ccgataagga tcggtttcgc cagtcccagc gttaccagtt cctgagtggc atgcagaacg
      961 cgcgcctctt ccccttccgg cagaacaacg cgcttcggcg ctttgcgagc ctgggagaaa
     1021 atcggcttca taaacaggtt ggttttgtaa acgaactcag tcagcttgtc gatgtagacg
     1081 tcgaaatcag caatcggacg agtcgccacg cccgactcca tcgcggcttt agcgaccgca
     1141 ggagcgatct taacgatcaa gcgcggatca aacggttttg gaatgatgta ttccggacca
     1201 aagctcagat cctgatcgcc atacgctgaa gccaccactt cgctctgttc cgcatgggcg
     1261 agttctgcaa tcgcacgtac cgccgccagt ttcatctctt cgttgatggc ggttgcgcca
     1321 acgtccagcg cgccacggaa gatgaacggg aagcacagga cgttgttcac ctggttcgga
     1381 tagtcagaac gaccggtgca aatgatggca tccggacgca cttctttcgc cagcggcggc
     1441 agaatttccg gttccgggtt cgccagcgcc aggatcattg gcgcacgagc cattttcttc
     1501 accatttcct gggtcagcac tttcgggccg gaacagccca ggaaaatatc cgcgccttca
     1561 atcacatcat cgagggtacg tttgccgtca tccaccaccg catatgcggc tttggtttcc
     1621 gccatgtttg gctcacggcc ctgatagata acgccttttg aatcgcaaac cacgatgtta
     1681 tgtttttgca gacccagcgc taccagcagg ttcatacagg cgattgctgc ggcacccgcg
     1741 ccggaaacca ccatccgcac gtcggagatg tttttctcca ccacgcgcaa gccgttgagg
     1801 atggcggcag tgctgataat tgccgtgccg tgctgatcgt cgtggaatac cggaatattc
     1861 atccgctcgc gcagtttctg ttcaatatag aaacattctg gcgctttaat gtcttcgagg
     1921 ttgatgccgc cgaaggttgg ttcgagcgcg gcgacaactt caataaattt gtccgggtcg
     1981 agttcgtcaa cttcaatgtc aaatacatca atcccggcga atttcttaaa cagaacgccc
     2041 ttgccttcca tcaccggttt gcctgccagc gcgccaatgt tgcctaaccc cagcaccgcc
     2101 gtaccgttag agatcaccgc caccaggtta ccgcgggcgg tatatttgta ggcttttaac
     2161 gggtcttttt cgatttcaag acaaggtgcg gcaacgcctg gtgagtaggc cagcgccaga
     2221 tcgcgctgtg ttgccagagg cttggttgga gaaacctgga ttttccctgg aactggaaat
     2281 tcatggaaat caagtgcact ttgttttaac tggtcatcca tttggttgtt cctttcacgt
     2341 aacgttcaca aataaagtgt gtgggcaaca gcccctgccc acaacgtggc gcacattatt
     2401 accctgccgg agtctacaga ctttgagcaa gtccaaactc tcaccattaa tataatgttt
     2461 tggtaataat cctataacac tgatgttacc tgcttaatcc agcaatacca tgcctgtctg
     2521 ctatgctttt ttgatgcgtt tagcgaaatt tctcagaagt gtgaattaac gcactcatct
     2581 aacactttac ttttcaagga gtatttccta tgaacgagtt agacggcatc aaacagttca
     2641 ccactgtcgt ggcagacagc ggcgatattg agtccattcg ccattatcat ccccaggatg
     2701 ccaccaccaa tccttcgctg ttactcaaag ctgccggatt atcacaatat gagcatttaa
     2761 tagacgatgc tatcgcctgg ggtaaaaaaa atggcaagac ccaggaacaa caggtggtcg
     2821 cagcgtgtga caaactggcg gtcaatttcg gtgctgaaat cctcaaaatc gtacccggtc
     2881 gcgtgtcaac agaagttgat gcacgcctct cttttgataa agaaaagagt attgagaagg
     2941 cgcgccatct ggtggacttg tatcagcaac aaggcgttga gaaatcacgc attctgatca
     3001 agctggcttc gacctgggaa ggaattcgcg cggcagaaga gctggaaaaa gaaggtatta
     3061 actgcaacct gacgctgctg ttttcttttg cacaggcacg ggcctgtgcg gaagcaggcg
     3121 tttttctgat ttcgccgttt gtcgggcgta tttatgactg gtatcaggca cgcaagccga
     3181 tggacccgta tgtggtggaa gaagatccgg gcgttaaatc ggtgcgcaat atctacgact
     3241 actataagca acaccactat gaaaccattg tgatgggcgc gagcttccgt cgcaccgaac
     3301 aaatcctcgc cttaaccggc tgcgatcgac tgactatcgc accgaattta ctgaaggagc
     3361 tgcaggaaaa agtttcgcca gtggtacgta aattaatccc accttctcag acgttcccac
     3421 gcccagctcc catgagcgaa gcggagttcc gttgggagca caatcaggat gcgatggcgg
     3481 tagaaaaact gtctgaaggc attcgtctgt tcgccgttga tcaacgcaaa ctggaagatc
     3541 ttcttgccgc caaactataa accagccacg gagtgttata tgtcccgaaa agaccttgcc
     3601 aatgcgattc gcgcactcag tatggatgcg gtacaaaaag ccaactctgg tcatcccggc
     3661 gcgccgatgg gcatggctga tattgccgaa gtgctgtgga acgattttct taaacataac
     3721 cctaccgacc caacctggta tgatcgcgac cgctttattc tttccaacgg tcacgcgtcg
     3781 atgctgctct acagtttgct acatctgacc ggttacgacc tgccgctgga agaactgaag
     3841 aacttccgtc agttgcattc gaaaacccca ggccacccgg agattggcta tacgccaggc
     3901 gttgaaacca ccaccggccc gcttggacaa ggtttggcga acgccgtcgg gctggcgata
     3961 gcagagcgta cactggcggc gcagtttaac cagccagacc atgagatcgt cgatcacttc
     4021 acctatgtgt ttatgggcga cggctgcctg atggaaggta tttcccacga agtctgttcg
     4081 ctggcaggca cgctgggact gggcaagctg attggttttt acgatcacaa cggtatttcc
     4141 atcgacggtg aaacagaagg ctggtttacc gacgatacgg caaaacgttt tgaagcctat
     4201 cactggcatg tgatccatga aatcgacggt cacgatccgc aggcggtgaa ggaagcgatc
     4261 cttgaagcgc aaagcgtgaa agataagccg tcgctgatta tctgccgtac ggtgattggc
     4321 tttggttcgc cgaataaagc aggtaaggaa gaggcgcacg gcgcaccact gggggaagaa
     4381 gaagtggcgc tggcacggca aaaactgggc tggcaccatc cgccatttga gatccctaaa
     4441 gagatttatc acgcctggga tgcccgtgaa aaaggcgaaa aagcgcagca gagctggaat
     4501 gagaagtttg ccgcctataa aaaggctcat ccgcaactgg cagaagagtt tacccgacgg
     4561 atgagcggtg gtttaccgaa ggactgggag aaaacgactc agaaatatat caatgagtta
     4621 caggcaaatc cggcgaaaat cgctacccgt aaggcttcgc aaaatacgct taacgcttac
     4681 gggccgatgc tgcctgagtt gctcggcggt tcggcggatc tggctcccag caacctgacc
     4741 atctggaaag gttctgtttc gctgaaggaa gatccagcgg gcaactacat tcactacggg
     4801 gtgcgtgaat ttggcatgac cgctatcgcc aacggcatcg cgcaccacgg cggctttgtg
     4861 ccgtataccg cgacgttcct gatgtttgtt gaatacgccc gtaacgccgc gcggatggcg
     4921 gcactgatga aagcgcggca gattatggtt tatacccacg actcaattgg cctgggcgaa
     4981 gatggtccga cgcaccaggc tgttgagcaa ctggccagcc tgcgcttaac gccaaatttc
     5041 agcacctggc gaccgtgcga tcaggtggaa gcggcggtgg gctggaagct ggcggttgag
     5101 cgccacaacg gaccgacggc actgatcctc tcaaggcaga atctggccca ggtggaacgt
     5161 acgccggatc aggttaaaga gattgctcgt ggcggttatg tgctgaaaga cagcggcggt
     5221 aagccagata ttattctgat tgccaccggt tcagagatgg aaattaccct gcaagcggca
     5281 gagaaattag caggagaagg tcgcaatgta cgcgtagttt ccctgccctc gaccgatatt
     5341 ttcgacgccc aggatgagga atatcgggag tcggtgttgc cttctaacgt tgcggctcgc
     5401 gtggcggtgg aagcaggtat tgccgattac tggtacaagt atgttggtct gaaaggggca
     5461 attgtcggga tgacgggtta cggggaatct gctccggcgg ataagctgtt cccgttcttt
     5521 ggctttaccg ccgagaatat tgtggcaaaa gcgcataagg tgctgggagt gaaaggtgcc
     5581 tgatggtgat tgccggatgc tgattgccgg atgcgacgct gacgcgtctt atccggccta
     5641 catgtcccgc cattttgttt aacgggtgat ccacaacgtg ggccaggcgt ctggcccatg
     5701 ccagttatcg caggtgggtt ctgcggcgta acgcaccagg cgaaaacgct gaccgtcaaa
     5761 gcgccagcgc gcctgaatgc cacaatcgct taatccgcgc cctttcgcta aggtcaccag
     5821 ttcacgcgat ttctcatcaa atgttgcgtt catcagttcc agttcattcg tctcctgacc
     5881 gttgttgaac ggcaaacgca accgaaccgg gcgcgaagct agtggctttt tacgcgacac
     5941 aatccatgcc aaatcaatgg tgttataggc ccctgcctca cagctaatca tcatcagcgc
     6001 tttatcatca gtcagcgcag tgacattcac ctcacgacgc aatggatcaa gcgagcagcg
     6061 cagaccattc atccgccagt ttccataatc cagcaaatcg ttgcgttctt cgagtgagag
     6121 tggcgtcggc gttgggttaa ccaccgcgac ctctttcagc gcaggcgcgg gcggtacgct
     6181 gagcggcggt tcgtcccctt tcttgatcca cgcggtttca ctgccaacgc gcttttgctg
     6241 agcatcaata aacaacaacg ctgcttttaa gccactcaga gaaatggtct gatcgccatc
     6301 gcgtaaggtg attgccttcc cttcctgaat catctgcaaa aacgcggtga tggttgccgt
     6361 atcatcggtt actaataacc atggtgaaat ccgccacttg tcaccactta acgccaacgg
     6421 ctcgccatct aacagcagcc gtggcgctat ctccccttct gacgcctccg gcgacttcaa
     6481 tccgccgcgc tcaatacgta aaacggcatc ggtatgcgcc ccggcgctgc ggctcagggt
     6541 catcaccagt ccattatgat cgcccgtatt acgcgccacg cagaaatttt ggttattaca
     6601 ggtgacctgc cagtcggaaa acgcccgttg cgctggtgcc gcccacacca aagacgtcgg
     6661 caacaaagcg aaaaaaaaga gaaggaaaat gcgatagcgc atggacggta cgaccccaga
     6721 agttcaaaca aacagcacaa gtcgtatctt cgttgcgcag gtagcgcggc tcaatcggat
     6781 ttgtcggatt gagccaataa taaacaacga aatattattg ttatttttca gtccattaaa
     6841 tgtgacgttt gcaaatagtt aagcaataac accgtcttac cgtcacgtat ctcgccggtt
     6901 ttgatcatct ccagcgcctg gctgaacggc agctcgagca cttcaatatc ttcatcttcg
     6961 acaccgccac cggcgttagc gcgctgattg tcactgtatt cggcgataaa aaagtggatt
     7021 agctcagtca caccgcctgg cgacatatac agttcaaata atttgcgcac ttcacccact
     7081 tcatagcccg tctcttcaat cgcttctttg cgaatacaca cttccggttc gtcgttatcc
     7141 agcagcccgg cgcagctttc aatcagctgc ccgctttcat tgccattaac ccaggtagcg
     7201 acacggaact gacgaatcag aaccacggtc tttttcttcg tgttgtacag gaggatcgtc
     7261 gcaccattgc cgcgatcgta cacttcacgt ttatggcgga taacttcgcc gtctttgcgc
     7321 gtgagatcgt aagtaatgtt gtgcagggtg aaatagttat cggagagaat tttgtcttta
     7381 atgagggtga tttgttgcgt cataccgact ccacagcgcg aaatgaacaa ttatcttacg
     7441 ctgtgaagtc gggtttgtct gcgcaggcta tcagtgagat ttgactgatt ttacccccag
     7501 ccagtcaata atcccctgtg ccgcatgacg tccttctgcc attgcggtaa ccaccagatc
     7561 cgcaccacgc acggcgtcac caccagcgaa gattttcgga ttggtggtct ggtaacggta
     7621 ctggctttcc acatccgcga tgatgcggcc ccatttgtct accgttacac cgtgcgactc
     7681 cagccacggc atcccgtgcg gattgaagcc aaacgccata atcaccgcgt cggctggcat
     7741 gacaaattca ctgccttcca ccggcactgg acgccgacgc ccctgggcat ccggctcgcc
     7801 aagacgcgtg cgcaggaaac gaatcccgca gacgtgacct tgttcattca gctcaagcgc
     7861 caccggctgg acgttaaatt cgaagttggc cccctcttcg cgggcgttct tcacttcttt
     7921 cttcgagcct ggcatgttag cttcatcacg acgataagcg caggtgacgt tactcgcgcc
     7981 gtggcgcagt gcggtacgca cacagtccat cgcggtgtcg ccgccgccca gtaccacgac
     8041 gttaagtccg gcggtattga taaacggctc ttccggtagc tcttcgagcc ccatcacctg
     8101 tttagtgttg gcaatgagga acggcagcgc gtcataaacg cccggcgcat cttcattggg
     8161 taaacccgct ttcatggaac ggtaagtgcc tacgccaacg aagaccgcgt cgtattgttc
     8221 caaaagcgaa tccaaagaga catctttacc cacttcacaa ttgagttcga agtgaatccc
     8281 catcgcgctg aagatttccc gacggcgtgc cagcagggat ttatccagtt tgaaagaagg
     8341 aatgccgaaa gtgagcaagc caccgatttc tggatggcga tcgtacaccg tcacccccac
     8401 gccattgcgg gtcagaacat ccgcacaggc cagccctgcc ggacctgcac cgataatcgc
     8461 cacccgcttg tccactttgg tgacatggct taagtcagga cgccaacctt tcgccaacgc
     8521 ctgatctgaa atgtagcgtt caatgttgcc gatagttacc gcgccgtgct catcgcgaat
     8581 agtacaggca ccttcacaca aacggtcttg cggacaaacg cgtccggtaa tttccggcag
     8641 ggtgttggtc tggtgagaaa gctcgacggc ggcgtcgatg tttccggctt tcaccagttc
     8701 aatccactgc ggtatatggt tatgcagcgg gcaggtccat tcacaaacgc tgtgctcgcc
     8761 gcacttaagg cagcgcgagg cttcccgttg tgcctggtcg gcgcgaaatg gcagataaat
     8821 ttcatcaaaa ccggttttgc gcgcttcaat cgccagttta tccggctcgc cacgcgcggg
     8881 cgttgcctgc atttgttcga ctttactcat taccggcatt tcttgcgccg cggtactggc
     8941 atgccacggt tgatgttcct gacgcgcggt gcgcaagcgg cgggatttcg ccatgccgga
     9001 gagtgcgacg tcagtgacca gttgcagcgc gtccgccggg caattctcaa cacaggcagg
     9061 accgttttcg cgccccgcac aaaggtcgca tttatgcgcc gtggctttta cttttcctgc
     9121 cgcgacgggt gtcaggacga tttgcatcgt accaaaagga caggccacca cgcaggattt
     9181 acagccaata cacttttgct gattgacctg aatgctgtca tcaacgtggc tgattgcgcc
     9241 attagggcag ctacgggcgc agggcgcatc ttcacaatgg tgacaggtca ctgcactacg
     9301 ttgctgttga tgtttgataa ccgtaattcg gggatgaaaa tggtgttggc tcaggacatg
     9361 ttgctcatca ttgtgagcca tgacacaggc gatttcacaa gcatgacaac ccagacattg
     9421 ctgactgttg gccataataa aacgattcat aacgaccttc ttttttggtt gtaaaaacct
     9481 tattctttat atgagtgttg ttattacccg acttacaggg gaatcggcaa tgttcatgtg
     9541 cccagaataa gtaactattt cgctataaac tgtggcagat caaataatcc catcactgac
     9601 taaattgcgt ttcagcgaac tggaacatta atgatttttt gtggagaaga cgcgtgtgat
     9661 tgttaaacga cccgtctcgg ccagtctggc ccgggccttt ttttacattg tgctgctgtc
     9721 gattctttcc acgggtatcg ctctgctaac tctggcgagc agtttgcgcg acgctgaggc
     9781 tatcaatatt gccggatcgc tgcgtatgca gagttaccgc ctgggctacg acttgcaaag
     9841 tggcagtcca caactcaatg cacatcgcca gttatttcag caggcactgc attcaccggt
     9901 attaaccaac ctcaacgtct ggtatgtgcc agaagcagta aaaactcgct atgcgcatct
     9961 gaatgccaac tggctggaga tgaataatcg gctcagcaag ggcgatttgc cgtggtatca
    10021 ggccaatatt aataattatg ttaatcagat agacctgttc gtactggctt tacagcacta
    10081 cgctgaacgc aaaatgctgc tggtggtggc gatttccctg gctggcggca tcggtatttt
    10141 cacgctggtc ttttttactt tgcgccgcat acgccatcag gtggttgccc cgctgaatca
    10201 gctggttacc gccagtcagc gtattgaaca cgggcagttc gactcgccgc cgctggatac
    10261 caacctgccg aatgagcttg gtctgcttgc aaaaaccttt aaccagatgt cgagcgagct
    10321 gcataaattg taccgttcgc tggaagcgtc agtagaagaa aagacccgcg atctccacga
    10381 ggccaagcgt cgtctggagg tgttgtatca gtgttcgcag gcgctgaaca ctagccagat
    10441 tgatgtgcat tgtttccgcc atattttgca gattgttcgc gacaatgaag cggctgaata
    10501 tctggagtta aatgtcggtg aaaactggcg gattagcgaa gggcaaccaa acccggaatt
    10561 gccgatgcag attttaccgg tgacaatgca agagacggtt tacggcgaac tgcactggca
    10621 aaatagtcac gtttcatcat cagaaccgct gcttaacagc gtttcgtcga tgctgggacg
    10681 cggtttgtac tttaatcagg cgcagaagca ttttcagcaa ttattgttga tggaagaacg
    10741 tgcgaccatc gcccgcgaat tgcacgactc gctggctcag gtactttctt acttacgtat
    10801 ccagttgacg ttactgaagc gttcgatacc ggaagataac gccaccgcac aaagtatcat
    10861 ggccgatttt tcccaggcgt tgaatgatgc ttatcggcag ttacgcgagc tgttgactac
    10921 tttccgcctg acgctgcagc aggcggatct cccctccgcg ttgagggaaa tgctggatac
    10981 gttacaaaat caaaccagcg ccaaactgac cctcgactgc cgtctgccaa ccctggcgct
    11041 ggatgcgcaa atgcaggtgc atttgttgca aattattcgc gaagcggtgc tgaatgcgat
    11101 gaagcacgcc aacgccagcg aaatcgccgt cagttgcgtc accgcgccgg acggcaatca
    11161 cacggtttat atccgtgata acgggattgg tatcggtgaa ccgaaagaac ccgaaggtca
    11221 ttatggtctg aatatcatgc gcgaacgcgc ggaacggctg ggtgggacgc tgactttttc
    11281 acaaccttcc ggcggcggca cgttagtgag tattagcttt cgctctgcgg agggtgagga
    11341 aagtcagttg atgtaaagcc tcctactgac caaagaatac ttgcacttaa ggttcagtat
    11401 aaaagggcat gataatttac attagctcct ttttttctcc acgattggct cgtaccttgc
    11461 cgctacagtg aagcaagtca agcctacaac gatacgcaga aacacgaggt cctcttttaa
    11521 tggcgaattt ctttattgat cgccccattt ttgcctgtgt gctggcaatc ctgttgtgtc
    11581 tgacaggtac cctggcgatt ttctcattgc ccgttgaaca ataccccgat ctcgcgccac
    11641 cgaatgtgcg agtgaccgct aactatcccg gcgcatcggc ccagacgctg gaaaacaccg
    11701 tgacccaggt tatcgagcaa aatatgaccg gcctcgataa tctcatgtat atgtcatctc
    11761 agagcagtgg caccggtcag gcatctgtca ctttaagttt taaagcaggc accgatccgg
    11821 acgaagccgt gcagcaagta caaaaccagc tgcaatcagc catgcgaaag ttaccgcagg
    11881 cggtgcaaaa tcagggcgtg acggtgcgta aaaccggcga taccaacatt ctgaccattg
    11941 ccttcgtctc taccgatggt tcgatggata aacaggatat tgctgattat gttgccagta
    12001 atattcagga cccgttaagc cgcgtgaatg gcgtcgggga tatcgatgcc tatggttcgc
    12061 aatattccat gcgtatctgg ctggacccgg cgaaactcaa cagtttccag atgacggcta
    12121 aagatgtcac tgatgccatt gagtcacaga acgcgcagat tgcggttggg caacttggtg
    12181 gtacaccttc cgtcgataag caggcgctca acgccaccat taacgcccag tcactgctgc
    12241 aaacaccaga acagttccgc gatatcacct tgcgggtcaa tcaggacggc tcagaggtaa
    12301 ggctgggcga tgtcgccacc gtcgaaatgg gggcggagaa atacgattat cttagccgct
    12361 tcaatggtaa gccagcctcc gggctggggg taaaactggc ctccggcgct aacgaaatgg
    12421 cgacagcgga gctggtgctc aatcgtctcg acgagctggc gcagtatttc ccgcatggac
    12481 tggaatacaa ggtggcgtat gaaaccacct cgtttgttaa agcctccatt gaagacgtgg
    12541 tgaaaacgct gctggaagct atcgctctgg ttttcctcgt tatgtatctg ttcctgcaaa
    12601 acttccgcgc cacgctgata cccactatcg ccgtgccggt ggtgttgatg ggaaccttct
    12661 ccgtacttta cgccttcggt tacagcgtca acaccttaac catgttcgcg atggtgctgg
    12721 cgatcggtct gctggtggat gacgccatcg tggtggtgga aaacgtcgaa cgtattatga
    12781 gtgaggaagg actcactcct cgcgaagcca cacgtaaatc gatggggcag atccaggggg
    12841 cactggtcgg gattgcgatg gtgctttcgg cggtatttgt accaatggcc ttcttcggcg
    12901 gcaccaccgg tgccatctat cgccagttct ctattaccat tgttgcggcg atggtgctgt
    12961 cagtactggt agcgatgatc ctcactccgg ctctgtgtgc cacactactt aagccactga
    13021 aaaaaggtga gcatcatggg caaaaaggct tttttgcctg gtttaaccag atgtttaacc
    13081 gcaacgccga acgctacgaa aaaggggtgg cgaaaattct ccaccgtagc ctgcgctgga
    13141 ttgtgattta tgtcctgctg cttggcggca tggtgttcct gttcctgcgt ttgccgacgt
    13201 cgttcttacc gctggaagac cgtggcatgt ttactacctc ggtacaattg cccagcggtt
    13261 caacccaaca acagaccctg aaagtcgttg agcaaatcga gaaatattac ttcacccatg
    13321 aaaaagacaa catcatgtcg gtgtttgcca ccgttggctc tggccctggg ggcaacgggc
    13381 aaaacgtggc gcgaatgttt atccgcctga aagactggag cgaacgcgac agtaagaccg
    13441 gcacctcgtt tgccattatc gagcgtgcaa cgaaggcgtt taacaagatt aaagaagctc
    13501 gcgttatcgc cagcagcccg ccagcaatta gcggtcttgg cagttctgcg ggttttgata
    13561 tggagttgca ggaccacgct ggagcaggtc acgatgcgct gatggcagca cgtaaccagt
    13621 tgctggcgct ggcggcagaa aacccgaagc taacccgtgt gcgccataac ggcctcgacg
    13681 acagtccgca gttgcagatt gatatcgacc agcgtaaagc tcaggcgctg ggcgttgcta
    13741 tcgacgatat taacgacacg ctgcaaaccg cctggggttc gagctatgtg aatgacttta
    13801 tggatcgcgg tcgcgtgaag aaagtctatg tgcaggcagc tgcgccgtat cgcatgctgc
    13861 cagatgacat taatctctgg tatgtccgaa ataaagatgg cggcatggtg cccttctctg
    13921 ctttcgcgac ctcacgctgg gagacaggct cgccgcgtct ggaacgctat aacggttatt
    13981 ctgcggttga gattgttggg gaagccgcac cgggggtcag taccggtacg gcgatggata
    14041 ttatggaatc gttagtgaag cagctgccaa acagctttgg tctggagtgg acggcgatgt
    14101 cgtatcagga gcggctttcc ggcgcgcagg ctccggcgct gtacgctatt tcattgctgg
    14161 tggtattcct gtgtctggcg gcgttgtatg aaagctggtc ggtgccgttc tcggtaatgc
    14221 tggtcgtgcc gctgggggtt atcggcgcgc tgctggcaac ctggatgcgg gggctggaaa
    14281 acgacgttta cttccaggtg ggtctgttaa cggtcattgg tttatcggcg aaaaacgcca
    14341 tcctgatcgt cgagtttgct aacgagatga accaaaaagg ccacgacctg tttgaagcga
    14401 cgctccacgc ctgccgtcag cgtttacgcc cgattctgat gacctcgctg gcatttatct
    14461 tcggcgtatt gccaatggca accagcacgg gtgccggttc cggtggtcag catgcggtgg
    14521 gtactggcgt aatgggcggg atgatttcgg ctactattct ggctatttac ttcgtgccgc
    14581 tgttctttgt gctggtgcgc cgccgcttcc cgctgaagcc gcgcccggaa taagcaataa
    14641 aaaaggcgac atgccaatgt gtcgcctttt tcaactttcc gattaagaac ttgctcagca
    14701 ggttcttgct gtttgtactt tgtctcagga attacttacg aagcataact tcaataaagt
    14761 ctttccagtt ccccagttca cgttcaatca taacaacctc tcttataatt atgggtattc
    14821 tacggaaaca atataccgtg gtgaagctaa tttactcgat tgctgcgatg actacctccg
    14881 ggggacaaac cttatgtaaa tactatggtc ctgcggtgat gatttgtatg tgatacacag
    14941 caacattt
//
LOCUS       DAPHBJ010000103        14807 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.104, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000103 DAPHBJ010000000
VERSION     DAPHBJ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.104"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(17..169)
                     /gene="hokE"
                     /locus_tag="RHZ76_002822"
     CDS             complement(17..169)
                     /gene="hokE"
                     /locus_tag="RHZ76_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HDV2813433.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            622..1740
                     /locus_tag="RHZ76_002823"
     CDS             622..1740
                     /locus_tag="RHZ76_002823"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDV2813434.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAATDHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1806..2054
                     /locus_tag="RHZ76_002824"
     CDS             1806..2054
                     /locus_tag="RHZ76_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HDV2813435.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVSTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            2119..2487
                     /locus_tag="RHZ76_002825"
                     /pseudo
     CDS             2119..2487
                     /locus_tag="RHZ76_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
     gene            2581..3234
                     /gene="nfsB"
                     /locus_tag="RHZ76_002826"
     CDS             2581..3234
                     /gene="nfsB"
                     /locus_tag="RHZ76_002826"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HDV2813436.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            3342..4589
                     /gene="ybdG"
                     /locus_tag="RHZ76_002827"
     CDS             3342..4589
                     /gene="ybdG"
                     /locus_tag="RHZ76_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HDV2813437.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4657..6033)
                     /gene="pheP"
                     /locus_tag="RHZ76_002828"
     CDS             complement(4657..6033)
                     /gene="pheP"
                     /locus_tag="RHZ76_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HDV2813438.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWMEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6135..9278)
                     /gene="cusA"
                     /locus_tag="RHZ76_002829"
     CDS             complement(6135..9278)
                     /gene="cusA"
                     /locus_tag="RHZ76_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HDV2813439.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAVPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(9290..10513)
                     /gene="cusB"
                     /locus_tag="RHZ76_002830"
     CDS             complement(9290..10513)
                     /gene="cusB"
                     /locus_tag="RHZ76_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HDV2813440.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWVAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(10529..10861)
                     /gene="cusF"
                     /locus_tag="RHZ76_002831"
     CDS             complement(10529..10861)
                     /gene="cusF"
                     /locus_tag="RHZ76_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HDV2813441.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(11019..12392)
                     /gene="cusC"
                     /locus_tag="RHZ76_002832"
     CDS             complement(11019..12392)
                     /gene="cusC"
                     /locus_tag="RHZ76_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HDV2813442.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIDIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGG"
     gene            12549..13232
                     /gene="cusR"
                     /locus_tag="RHZ76_002833"
     CDS             12549..13232
                     /gene="cusR"
                     /locus_tag="RHZ76_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HDV2813443.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            13222..14670
                     /gene="cusS"
                     /locus_tag="RHZ76_002834"
     CDS             13222..14670
                     /gene="cusS"
                     /locus_tag="RHZ76_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HDV2813444.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSQGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     PSEAIVVRCQTVNHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         3577 a         3981 c         3659 g         3590 t
ORIGIN      
        1 gctccccgca caacggctac ttcttcggtt cgtaagcgag aacagcctta aactcaatat
       61 tacgttcctt tacggtgaac tcacacagcg aatctccgac cagaagcgta aatcccagca
      121 ccgttaaaca cagcactatg actgccgcaa gggcatattt cgtcagcatg tttatattgc
      181 ctccatgcga agaagggact accatccaaa ttgttcgggt ttggagtggc agccccaggg
      241 ttgatagaaa tatctcctgg ggcttcatct ttccagacct cagagtaaac ctgaaaccgg
      301 aaagcttcag gcacccgccg ttatcttacc cttcctcaac aaaatagctg tttatttata
      361 cagcgagtcc tgtttcggac ttctcactcc tcatttaatc aactgaagct gaacccgttt
      421 ttttctgcat gactcaatcg tcaaaattct tcgccaccag tcagcactgc cataacttgt
      481 cacattgcaa aagtagccaa attgcttcag gcaccatcag ttcaccatca ccaaaaaaaa
      541 gaaacattcc agaacttccg catctcgccc agccgctccg acttctatac tgaatagaaa
      601 acgccaacat aagagaaacc tatgccatta cccgattttc atgtttctga accttttacc
      661 ctcggtattg aactggaaat gcaggtggtt aatccgccgg gctatgactt aagccaggac
      721 tcttcaatgc tgattgacgc ggttaaaaat aagatcacgg ccggagaggt aaagcacgat
      781 atcaccgaaa gtatgctgga gctggcgacg gatgtttgcc gtgatatcaa ccaggctgcc
      841 gggcagtttt cagcgatgca gaaagtcgta ttgcaggcag ccacagacca tcatctggaa
      901 atttgcggcg gtggcacgca cccgtttcag aaatggcagc gtcaggaggt atgcgataac
      961 gaacgctatc aacgcacgct ggaaaacttt ggttatctca ttcagcaggc gaccgttttt
     1021 ggtcagcatg tccatgttgg ctgcgccagt ggcgatgacg ccatttattt gctgcacggc
     1081 ttgtcacgat ttgtgccgca ctttatcgcc ctttccgccg cgtcgccata tatgcaggga
     1141 acggatacgc gttttgcctc ctcacgaccg aatatttttt ccgcctttcc tgataatggc
     1201 ccgatgccgt gggtcagtaa ctggcaacaa tttgaagccc tatttcgctg tttgagttac
     1261 accacgatga tcgacagcat taaagatctg cactgggata ttcgccccag tcctcatttt
     1321 ggcacggtgg aggttcgggt gatggatacc ccgttaaccc ttagccacgc ggtaaatatg
     1381 gcgggattaa ttcaggccac cgcccactgg ttactgacgg aacgcccgtt caaacatcag
     1441 gagaaagatt acctgctgta taaattcaac cgtttccagg cctgtcgcta tgggctggaa
     1501 ggcgtcatta ccgatccgca cactggtgat cgtcgaccgc taacggaaga taccttgcga
     1561 ttgctggaaa aaatcgcccc ttccgcacat aaaattggcg catcgagcgc aattgaagca
     1621 ctgcatcgcc aggtcgtcag cggtctgaat gaagcgcagc tgatgcgcga tttcgtcgcc
     1681 gatggcggct cgctgattgg gctggtgaaa aagcattgtg agatctgggc cggtgactaa
     1741 gtcgggaatt gccttacaga agaatatccc tgacaatggt gttttaactt acatttaaca
     1801 atgttatgaa acacccttta gaaaccttga ccaccgcagc aggcattttg ctgatggctt
     1861 tcctctcttg cctgctgctt cccgcccccg cattggggct gacgctggca caaaaactgg
     1921 tgagcacgtt ccatctgatg gatcttagtc agctttacac tttattgttt tgtctgtggt
     1981 ttttagtgct gggcgctatt gagtattttg ttctgcgctt tatctggcga cgctggttct
     2041 cgctggcgga ttaaacgtcg attatcgccg tacttcgcgg catactttgc ttattctcct
     2101 tcgcctaaag gaacgtttat ggataagcaa tcactgcacg aaacggcgaa acgcctggcc
     2161 cttgagttac cctttgtcga gctttgctgg ccttttggcc cggagttcga tgttttttaa
     2221 attggcggca agatttttat gctgtcgtcg gagctacgcg gcgtcccctt tatcaatctg
     2281 aagtccgatc cacaaaaatc cctgttaaat cagcaaatat acccaagcat taagccaggg
     2341 tatcacatga ataaaaagca ctggatttcg gtgtatcccg gcgaggaaat ctccgaagcg
     2401 ttacttcgcg atctgatcaa cgattcgtgg aatctggtgg ttgatggtct ggctaaacgc
     2461 gatcaaaaaa gagtgcgtcc aggctaaagc ggaaatctat agcgcatttt tctcgcttac
     2521 catttctcgt tgaaccttgt aatctgctgg cacgcaaaat tactttcaca tggagtcttt
     2581 atggatatca tttctgtcgc cttaaagcgt cattccacta aggcatttga tgccagtaaa
     2641 aaacttaccc cggaacaggc cgagcagatc aaaactctcc tgcaatacag cccatccagc
     2701 accaactccc agccgtggca ttttattgtt gccagcacgg aagaaggtaa agcgcgtgtt
     2761 gccaaatccg ctgccggtaa ttatgtgttc aacgaacgta aaatacttga tgcctcgcac
     2821 gtcgtggtgt tctgtgcaaa aaccgcgatg gacgatgcct ggctgaagct ggttgttgac
     2881 caggaagatg ctgatggccg ctttgccacg ccggaagcga aagccgcgaa cgataaaggt
     2941 cgcaagttct tcgctgatat gcaccgtaaa gatctgcatg atgatgcaga gtggatggca
     3001 aaacaggttt atctcaacgt cggtaacttc ctgctcggcg tggcggcgct gggtctggac
     3061 gcggtaccca tcgaaggttt tgacgccgcc atcctcgatg cagaatttgg tctgaaagag
     3121 aaaggctaca ccagtctggt ggttgttccg gtaggtcatc acagcgttga agattttaac
     3181 gctacgctgc cgaaatcacg tctgccgcaa aacatcacct taaccgaagt gtaattctct
     3241 cttgccgggc atctgcccgg ctatttcccc tcagattctc ctgatttgca taaccctgtt
     3301 tcagccgtca tcataggctg ctgttgtata aaggagacgt tatgcaggat ttaatatccc
     3361 aggttgaaga tttagcgggt attgagatcg atcacaccac ctcgatggtg atgattttcg
     3421 gtattatttt tctgaccgcc gtcgtggtgc atattatttt gcattgggtg gtactgcgga
     3481 ccttcgaaaa acgtgccatc gccagttcac ggctttggtt gcaaatcatt acccagaata
     3541 aactcttcca ccgtttagct tttaccctgc aggggattat cgtcaatatt caggcggtat
     3601 tctggctgca aaaaggcacc gaagcggcag atattctgac tacctgcgcg cagttgtgga
     3661 tcatgatgta tgcgctgctt tcagtcttct cgttgctgga tgttattttg aatctggcgc
     3721 agaaattccc ggcagcatct cagttaccgc tgaaagggat atttcagggg attaaactga
     3781 tcggcgcgat tctggtcggc attttgatga tctcgctgct gattggtcag tcgccagcga
     3841 ttctgatcag cggtcttggt gcaatggctg ccgtgctgat gttggtattt aaagatccga
     3901 ttcttggtct ggtggcaggt attcagcttt ccgcgaacga tatgctgaaa ctgggcgact
     3961 ggctggagat gccgaaatac ggcgcggatg gcgcggtgat cgatattggg ttaaccaccg
     4021 tcaaagtgcg taactgggac aataccatta ccactattcc cacctggtct ctggtttctg
     4081 actcctttaa aaactggagc gggatgtcag catctggcgg gcgacgtatt aagcgcagta
     4141 tcagtattga tgtcaccagt attcgttttc ttgatgaaga cgaaatgcaa cgtctgaata
     4201 aagcgcattt gttaaagcct tatttaacca gccgccatca ggaaattaat gagtggaatc
     4261 gccagcaagg ttctacggag tcggtattaa acctgcgccg aatgaccaat attggaacct
     4321 ttcgtgccta tctgaacgaa tatctgcgta accatccgcg gattcgtaaa gatatgactt
     4381 taatggtacg ccaactggct ccaggtgata atggtttacc gctcgagatc tatgcgttta
     4441 ccaacaccgt ggtgtggctg gaatatgaaa gcattcaggc tgatatattc gatcacatat
     4501 ttgcgattgt cgaagagttt ggtctgcgac ttcatcagtc gccaaccggc aatgatattc
     4561 gctctctggc gggtgcattt aagcagtaat taaaaaaacc gctctcatcg aatggatgag
     4621 agcggacatc gttttgtcgg atgtagcgtg aatgccttat ttccgacgca gcgttttaaa
     4681 tgccacaaac aggaatacaa tccacaccgg cagcaggatc gctgacaagc gcatatcatc
     4741 catcgtgcac atcagcagca aaatcatgcc gaggaaggcg atgcaaagat agttgccgaa
     4801 cggataaagc agcgccttaa actgtgtttc acgcccctga cgtcgcatcg ctgcacgaaa
     4861 acgcagatgc gccagacaga tcataatcca gttcaacagc agcgttgcta ccaccagcgc
     4921 catcagcaga ccaaacgctt tttgcggcag cagatagttg attaacacca ccagcgaagt
     4981 gatcgctccg gaaagcatca gcgagttaat cggcacaccg cgacggctga cgcgagtcaa
     5041 aaacttcggc gcattaccct gcacagaaag gccaaacagc atgcggctgt tagagtaaac
     5101 cccgctgtta tacactgaca gcgatgctac cagaatgacg aagttcagcg cagaagctac
     5161 cacgttgctg tcgagattat ggaaaatcat cacaaacggg ctactgttgg atttcacttc
     5221 catccacgga tagagcgcca gtaaaaccac cagtgaaccg atgtaaaaca gcaggatgcg
     5281 atacaccacc tgatttaccg cttttggaat gcttttttcc ggatcgcgcg cttcagcggc
     5341 agtaatccca atcagctcca gaccgccgaa ggagaacata attaccgcca gcgacaaaat
     5401 cagcccattc cagccggtgg cgaagaaacc accgtagcgc cagaggttgt cgatactggc
     5461 tttctcgccg ccgtgaccag aaaacagcag ccacaggcca aagccgatca taccgatgat
     5521 cgccagcact ttaatcagcg caaaccagaa ctcggtttcg ccatataagc gcacgttcac
     5581 caggttaacg gcgttgatga taataaagaa ggcggcagcc caaatccacg ttggaacatc
     5641 cgggaaccag tactgcatat agatgcccgc agcggtcagc tctgccattc ccaccagcac
     5701 gaacattacc cagtagttcc agccagagag gaagcccgca aacggtcccc agtatttata
     5761 ggcaaagtgg gcaaatgaac cggataccgg ctcctcaacc accatttcgc caagctggcg
     5821 cataatcagg aaagcgatga tcccggcgac gccgtagccc agcaatacag ccggacccgc
     5881 catctgaatc gccgggccaa tgccaagaaa cagaccagta ccaattgcgc cacccaacgc
     5941 aatcagttga atatgacggt tatgtaatcc gcgatgaagc gtcggctctt gattcgacgc
     6001 agtatcttcc gatacggttg acgcgttttt cacgcctttc ccctgtgtgt cttttttgtt
     6061 gaggggcacc ttttaacatt tagtgcccat cgtcgcaaga cacaatccac acggttaaac
     6121 ggggtatcct gcttttattt ccgtacccga tgtcggtgca gccacatcag cttatacgcc
     6181 gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggcgcg
     6241 gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg
     6301 gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatat
     6361 aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggcacggcc
     6421 tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggcg
     6481 agggcgataa agccagtgcc cgtcgccacg gaaagatgaa agcccatcca ccacagcaac
     6541 cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc
     6601 cgacggaacg ccagatacaa cagcacgaag ataatcatca acgtcatcgg caccatgagt
     6661 ttaagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg
     6721 cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc
     6781 atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc
     6841 agcatcgacg gtccggtaga gactttaatg tcggccacgt ctgccagggt gatttgctgc
     6901 ttcatcggcg tcaggatcgg cagctggcgc agtgcctgcg gactatcgcg ccagctttgc
     6961 ggataacgca gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc
     7021 ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg
     7081 gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg ctcggcaagc
     7141 gcagaagcta cgcctggcac cgttcgcgct acttcttcaa tttgctcagc catcgcgtcg
     7201 atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag
     7261 agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc cggcagccgc
     7321 acggtgttat ccagttcctc aatgattttg tccatcgtca tgcctggccg ccactgctcc
     7381 tgcggcttaa gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt
     7441 tccgctttcc cggttttgcc aaatacccgt gccacttcag gtacgctcat aattagcttg
     7501 tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc
     7561 atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc
     7621 ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc
     7681 agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cgggttactg
     7741 ctttccggcg gaattttgcc acggatccag tagcccatca ggatcgggat cactacgatc
     7801 gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga
     7861 cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga
     7921 ctgataaata gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt
     7981 ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagccg tttatgcgca
     8041 ttctcgatca tgacgatagc agcatcgacc atcgccccga cggcaatcgc aatgccaccc
     8101 aacgacataa tattggcatt cagtccctgg aagtgcatga caataaaagc aatacacaac
     8161 cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag aaacagcgca
     8221 cagactaccg ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcg
     8281 cggtcaatga gctggctgcg atcgtatgtt gtaactatct ccacgccttc cggcagacta
     8341 cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg
     8401 gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttagttc ggcaatgccc
     8461 cggcgcatct ccgggccaat ctggaccttc gcaacatcgc gcagataaac gggcacgcca
     8521 ttttcactgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgcag atagccgctg
     8581 gcgcgcacca tatattccgc ttccgccagt tcgatcgacg aaccgcccgc ttcctggttt
     8641 gaagcatcca gcgcgctttt tacttcggcg agactgatgc catactgcgc caggcgctgg
     8701 ggatcgataa ccacctgata ctctttcacc acaccgccca ccgacgccac ttccgcaacg
     8761 tcagggatgg ttttcagctc atatttgaga aaccagtcct gtaatgagcg taaatcggcc
     8821 agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg
     8881 gcatctggcc ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgagg
     8941 tactccagca cccgcgaacg cgcccagtac ggatcggtgc catcttcgaa aatgacatac
     9001 acataagagt cgccaaactg tgagaaaccg cgcacagtct tcgcgccagg caccgacaac
     9061 atggtggtgg ttagcggata agtcacctga ttttcaacga tttgtggtgc ctgaccggga
     9121 tagctggttt taataataac ctgcacatcg gagagatccg gcagcgcatc cactggcgta
     9181 ttaatgatgg tccaggtgcc ccagatgctc agaaacaacg cgcccatcag caccaaaaaa
     9241 cggttcgcca ccgagcgacg aataatccat tcaatcattg gttattccct caatgcgcat
     9301 gggtagcact ttcagagcgc atccgctcca gtgcgccaga aatattggct tcggaatcaa
     9361 tcaggaacag gccgctggaa accacctttt caccttccgc cagaccagag cgtaatgcgg
     9421 tgacgccttg cgatgcctgg aaaacagcaa cgcgtttcgg tacaaagcgc ccgtcggcat
     9481 caacggtaat cacccgctgt tcgctgccgg tatcaatcag cgcttgtgac ggaatgagca
     9541 gcatcggttc gctggcggtg ttgagttgca accaggcgtt catgcccggt tttagcgcct
     9601 cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca tccacgccag
     9661 gtagcagcgt ccatttgcgg atggtgagtg ttttatccgg tcgcgccgga acggtgaggg
     9721 taaactgcga ggcatctttc accagccagg cgatagactc cgggatcgca gcagtgaccc
     9781 acaccgggtc cataccctga attttcgcta ccacgttatc tttggcgata ttcattcccg
     9841 cgcgcagatc aaacgcggtg atcacgccat caatgggcgc tttgagcgta aagcgagtct
     9901 ggattttttg cgtggcgatc aggcggcgaa tatccgcctc cggcattccc gccagtcgca
     9961 gccgctcaag aatgccttca gtctgggtcg ccgtaccgcc ggtttcgcgc agcagtaaat
    10021 actcactctg cgcttccacc cagtcaggaa tggtcaggtc gagaagcggt gtgccctttt
    10081 gtactttatc gcccacggta agcggataca ccttgtcgat aaacccggca gcgcgagcct
    10141 gcacaatggc atactgatac tcgttgtaac tgacattcgc cgggaaactc tgggcaaaag
    10201 tcagcggtcc gcgcgtgacg gtagccgttt tcacccccag attctgcgtc tgagtcgggt
    10261 caatgcgcac accagacgca gaactctctt catcggcata tttcggcacc agatccatat
    10321 ccataaacgg cgatttacct ggtttatcga accgcgtatt gggatacatt gggtcgtacc
    10381 agaataagat tttacgttct gcggtcgacg ttttttctgc aggcggttcc gcctttgcaa
    10441 cccaggtaaa acctgccgca gaaataatac cgcccgcgat catgctgccg ataataagcg
    10501 cgattttttt catctcatta aacctgggtt actggctgac tttaatatcc tgtaataaag
    10561 aaaggttgcc ctgctggaca aaattaaacg ccactttgtc gccggtttta atttcactca
    10621 ttttcgtctg cggggtgatg gtaaagcgca tggtcatctc cggccagttc acagcagcaa
    10681 tcggatcgtg atggatggtg atttttttgc tttccagatc aatacccttt accacgccag
    10741 tggcgctaat aacctgtggt tgtgcttcgc tcatggtttc atgatgatgt tcgttagcct
    10801 gggcattaaa gccaataacg gtaaacagac tgaacattgc gacttgcagt gcttttttca
    10861 tttattctct cctggagtta aaagtcatac tctaaataat tcgagttgca ggaaggcgac
    10921 aagcgagtga atccccagga gcttacataa gtaagtgact ggggtgagcg aacgcagacg
    10981 cagcacatgc aacttgaagt atgacgagta tattgctgtc aaccgccacc aagtgcggta
    11041 tacaaagaaa tttcgttaac ctgacgggca taattcaggt cgagtaaagt ttgtcgggtt
    11101 gcaaataaag aacgctcggc atccagcact tccagataac ttactgcgcc gtgctgatat
    11161 aatgcccgcg cccgttgcaa agtaatttgc agcgatgcca gataacgctg ctgggcgcta
    11221 atttgatcgt tcaggctttg acgtaatgcc agcgcatctg ccacttcttt aaaggcgttc
    11281 tggattttct gttcataatt caccaccgac tgctgctggc gaatttcggc gatatccaga
    11341 ttggcctggt tgcgtccggc attaaaaatg gggatatcaa ttttaggaat aaaattccac
    11401 atcccgctgc tggcgttaaa taatgacgac agatcgctgc tggcggttga tattccgctg
    11461 gtcaggctga tagacgggaa aaatgccgcg cgtgcagcgc caatattggc attagccgcc
    11521 attaacgcgt gttcagcttc cataatatcc gggcgctgca ataagatttg cgacgacaat
    11581 cccgccggta atttaacgct ttgcaggctg tcgctgttta ctgtctgcgc ttgcggcagc
    11641 ttgccgtagc ttcccaataa cagttgcaat gcattattcg cctgcgccag ttccccctga
    11701 cgtttagcga tgtcgctgcg ggtactttca atcaccccgc gagcctgttc cagcgccaga
    11761 acattactgc tacctgtcaa cagttgtttt tcgacaaacg catatgactg ctgataatta
    11821 cgcagcgttt cttcggctat ttgcaattgc gcatacgcta gctgctgatt gaaataactt
    11881 tgcgcgacat tagaaaccag caaaatatgc accgcgcgct gggcttcctc agtggctaaa
    11941 taattttggc gctcggcttc gctcatgttc tttaagcgac cgaaaaaatc gagatcaaag
    12001 ctggcgttaa ggccggtcga aaactcccgt gtcgtggcgg tattcccttt aagattaccg
    12061 ctccagctgc cgctgccctc gccattgagc tgtgggtagc ggtcggcatc ggtcagacga
    12121 tattgcgccc gtgcctcctg cactttcagc gtcgccatgc gcaaatcccg attattcacc
    12181 agcgcctcgc taatcagcgt cttcacctga ttatcaacaa aaaaggtgcg ccagcccgcg
    12241 ttctgatagt tatctgctgc gttaaccagg ccgttctggc tgagtgagaa ctgctgcggc
    12301 acgggcattg ccggacgctg ataatccggt gccagtgaac aaccggttag cgcaagggcc
    12361 acacaaaatg gcagaagttt acaaggagac ataggctcat aatttctggt gattttatac
    12421 cgccaacttt actcgccagg ctctgatttt ccggtgacag gaaaatgaca aaattgtcat
    12481 tttgccaata agcgattgcc atctgatccc gctactctag aattgcccgg gcaacatgcg
    12541 gaggaaatat gaaactgttg attgtcgaag atgaaaagaa aaccggagaa tacttgacca
    12601 aagggttaac cgaagccggt tttgtggtcg atttggccga caacgggctg aatggctacc
    12661 atctggcgat gaccggtgat tatgatctga taatcctcga tattatgctg ccggacgtga
    12721 acggctggga tatcgtgcgc atgttacgct ccgccaataa agggatgccg attctgttgc
    12781 ttaccgcgct tggcaccatt gaacatcgcg tcaaggggct ggagttgggg gcagatgact
    12841 acctggtgaa gccattcgct tttgctgaac tgctggcgcg ggtgcgcaca ttactgcggc
    12901 gcggggcggc ggtgattatc gaaagtcagt ttcaggttgc cgatttgatg gtcgatctcg
    12961 tcagccgcaa agtcacccgc agcggcacgc gcatcacttt gaccagtaaa gagtttactc
    13021 tgctggagtt cttccttcgc catcagggcg aagtgctgcc ccgctcgctt atcgcctcgc
    13081 aggtatggga catgaatttt gacagcgata ccaatgctat tgatgtggcg gtgaagcggc
    13141 tgcgcggcaa aatcgacaac gactttgagc cgaagctaat tcagaccgtg cgcggcgtgg
    13201 gttacatgct tgaggtgccg gatggtcagt aagccatttc agcgcccgtt ttcgctggca
    13261 acccgcctga ccttttttat cagcctggcc accatcgcgg cgtttttcgc ctttgcatgg
    13321 atcatgatcc actcagtaaa agttcatttt gccgagcagg atattaatga tttaaaagag
    13381 attagcgcca cccttgaacg ggtactaaat caccctgacg aaacgcaagc ccgacgctta
    13441 atgacgctgg aagatatcgt cagtggttat tccaatgtgt tgatttccct ggcagatagt
    13501 cagggtaaaa cggtgtatca ctcccccggt gcgccggata tccgcgagtt tacgcgtgac
    13561 gccatacccg ataaagacgc tcagggtggc gaggtgtatc tcctttccgg cccgacgatg
    13621 atgatgccag gccacggtca cgggcatatg gaacacagca actggcggat gattaacttg
    13681 ccggttggcc cgttggtgga cggcaaaccg atttatacgc tctacatcgc gctttcgatc
    13741 gattttcatc ttcattacat aaatgatttg atgaataaac ttattatgac cgcatcggta
    13801 atcagcatcc tgatcgtctt tatcgtactg ttggcggtac ataaaggtca cgcgccgatc
    13861 cgcagcgtca gccgtcaaat ccagaatatt acctcgaaag atctcgacgt tcgcctcgac
    13921 ccgcagaccg tgccgattga gctggaacag ctggtactgt cgttcaacca tatgatcgag
    13981 cgtattgagg atgtctttac ccgccagtcc aatttctcag cggatatcgc tcacgaaatt
    14041 cgcacgccga ttaccaatct catcacgcaa acggaaatcg ccctcagcca gtctcgcagc
    14101 cagaaggagc tggaagatgt gctctactct aatctcgaag agctgacgcg aatggcgaaa
    14161 atggtcagcg atatgctgtt tctcgctcag gccgataaca accagctaat ccccgaaaag
    14221 aaaatgctca acctggcgga tgaagtcggc aaagtgttcg attttttcga ggcgttagcg
    14281 gaagatcgcg gcgtggagtt gcgatttgtt ggcgacaaat gtcaggttgc gggcgatccg
    14341 ctgatgctgc gtcgggcgtt aagcaacctg ctttctaatg ccctgcgtta tacgccaccc
    14401 agtgaggcta ttgtagtgcg ctgccagacg gtcaatcatc aggtgcaagt ttccgtcgaa
    14461 aaccccggta cgcccattgc gcccgagcac ttaccgcgat tgtttgaccg tttctatcgc
    14521 gttgaccctt cccgccagcg aaaaggtgaa ggtagcggta ttgggctggc gatagtgaaa
    14581 tcgattgttg tcgcgcataa aggcacggtt gcggtaacgt cagatgcgcg ggggacaagg
    14641 tttgtgatta tgctgccgga gagagagtga ttattaagtt caaattcagg gcgtggtgca
    14701 tcacctcctg gtattgttga tcaacaaatt agagaaaaac ttattaaaca tatgaaatat
    14761 cgaaggcttt tatttttcaa agtttcacga gatgtttgta tatgaaa
//
LOCUS       DAPHBJ010000104        14628 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.105, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000104 DAPHBJ010000000
VERSION     DAPHBJ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.105"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            57..623
                     /locus_tag="RHZ76_002835"
     CDS             57..623
                     /locus_tag="RHZ76_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV2813445.1"
                     /translation="MFKGQKTLTALAISLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            674..3133
                     /locus_tag="RHZ76_002836"
     CDS             674..3133
                     /locus_tag="RHZ76_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV2813446.1"
                     /translation="MDTVNIYRLSFVSCLVMAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWQKKGDKTIPCINDSLVDKFGLKPDIRQ
                     SLPQIDRCIDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWAPPSTWKEGVAGVL
                     MDYNLFASNYRPQDGSSSTNLNAYGTTGINAGSWRLRSDYQLNNTDSEDSHEQSGGIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3148..3879
                     /locus_tag="RHZ76_002837"
     CDS             3148..3879
                     /locus_tag="RHZ76_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV2813447.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTAKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVFPGFKTVMIAPFSTVNTNTGSYSGSQFYLGYMD
                     DYGALRMTTLNCSGECRLQAVEAKK"
     gene            3876..4937
                     /locus_tag="RHZ76_002838"
     CDS             3876..4937
                     /locus_tag="RHZ76_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2813448.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEVIQPF
                     AVPGNAKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSDDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHNMTKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLIYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5089..6135
                     /locus_tag="RHZ76_002839"
     CDS             5089..6135
                     /locus_tag="RHZ76_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDV2813449.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQHIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAELQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6132..6923)
                     /gene="nei"
                     /locus_tag="RHZ76_002840"
     CDS             complement(6132..6923)
                     /gene="nei"
                     /locus_tag="RHZ76_002840"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HDV2813450.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKSYQSPLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTVDKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6959..7693)
                     /gene="pxpA"
                     /locus_tag="RHZ76_002841"
     CDS             complement(6959..7693)
                     /gene="pxpA"
                     /locus_tag="RHZ76_002841"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDV2813451.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLAAAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAENGIVVAA"
     gene            complement(7683..8615)
                     /gene="pxpC"
                     /locus_tag="RHZ76_002842"
     CDS             complement(7683..8615)
                     /gene="pxpC"
                     /locus_tag="RHZ76_002842"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HDV2813452.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLEDGDRLPIGKSKHDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8609..9265)
                     /gene="pxpB"
                     /locus_tag="RHZ76_002843"
     CDS             complement(8609..9265)
                     /gene="pxpB"
                     /locus_tag="RHZ76_002843"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDV2813453.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9288..10031)
                     /gene="ybgI"
                     /locus_tag="RHZ76_002844"
     CDS             complement(9288..10031)
                     /gene="ybgI"
                     /locus_tag="RHZ76_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HDV2813454.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10302..11783
                     /gene="dtpD"
                     /locus_tag="RHZ76_002845"
     CDS             10302..11783
                     /gene="dtpD"
                     /locus_tag="RHZ76_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HDV2813455.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            complement(11825..13243)
                     /gene="phrB"
                     /locus_tag="RHZ76_002846"
     CDS             complement(11825..13243)
                     /gene="phrB"
                     /locus_tag="RHZ76_002846"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HDV2813456.1"
                     /translation="MITHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13240..13749)
                     /locus_tag="RHZ76_002847"
     CDS             complement(13240..13749)
                     /locus_tag="RHZ76_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HDV2813457.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13900..14628)
                     /locus_tag="RHZ76_002848"
                     /pseudo
     CDS             complement(13900..14628)
                     /locus_tag="RHZ76_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3537 a         3891 c         3550 g         3650 t
ORIGIN      
        1 cattttatat attcttgcca cgcaacactc tatctcatta tttaaaggac tcagttatgt
       61 ttaaaggaca aaaaacattg accgctctgg ccatatctct gctattcact gcacctgttt
      121 atgcagctga tgaaggatcc ggtgaaattc actttaaagg tgaagttatt gaagcaccgt
      181 gtgaaataca tcaggatgat attgataaag aggttgaact cggtcaggtg accaccagcc
      241 acattaatca gtcacatcac agcgatgccg ttgctgtcga cctgcgctta gtcaactgtg
      301 atctggaaaa ctccagcaac ggttccggtg gcaagatttc taaagttgca gtcacctttg
      361 atagctcagc gaaaaccacc ggcgcagatc cgattctcaa caacaccagc acgggtgaag
      421 ccaccggcgt cggcgtacgt ttaatgaata aagatcaaag caatatcgtt ttaggcaccg
      481 ctactccaga tatcgacctg gctccgacct ccagcgaaca aacgctgaat ttctttgcct
      541 ggatggaaca aattgatcag gctacacctg taacgccagg cgcagttaca gcgaatgcga
      601 cctacgtgct ggattataaa taagtattac tttggcggcg ctatcgccgc ctttcttcaa
      661 atatttatca ggtatggaca ccgtgaatat ttatcgactc tcttttgtat cctgcctggt
      721 catggcgatg ccttgcgcat tggcggtcga atttaatctg aatgttctcg ataaatcaat
      781 gcgcgaccgc attgatattt cattattaaa ggaaaaagga gtcattgctc ccggtgaata
      841 ttttgttagc gttgcggtga ataacaacca aatcagtaat gggcaaaaaa tcaactggca
      901 aaaaaagggt gacaaaacca ttccatgcat caatgattca ctggtcgata aatttggttt
      961 aaaaccagat atccgtcagt ccttgccaca gatagatcgg tgtattgatt tcagttcccg
     1021 acctgaaatg ctcttcaatt tcgatcaagc caatcagcaa ctgaatatta gtattccgca
     1081 agcctggctg gcgtggcact cagaaaactg ggctcccccc tctacatgga aagaaggtgt
     1141 tgccggggtc ctgatggatt ataacctgtt tgccagcaac taccgcccac aggacggcag
     1201 cagcagcact aacctgaacg cctacggtac caccggaatt aacgccgggt catggcgctt
     1261 acgcagtgat taccagctta ataacaccga tagcgaagat agccatgaac agtcaggcgg
     1321 aatatcgcgc acctatcttt ttcgtccatt accgcaatta ggctctaaat taaccctcgg
     1381 cgaaaccgat ttcagttcca atattttcga tggtttttct tatactggcg cggcactggc
     1441 gagtgacgat cgaatgttgc catgggagct acgcggatac gccccacaaa ttagcggtat
     1501 tgcacagacc aatgccacgg tgacgatcag tcaatcaggt cgcgtcattt accagaaaaa
     1561 agtccctccg ggaccgttta ttattgatga cctcaatcag tctgttcagg gcacgcttga
     1621 tgtcaaagtg acggaagaag atggtcgggt gaacaatttc caggtttcgg cagcatcgac
     1681 gcccttcctg actcgtcagg gacaggttcg ctataaactg gccgcgggtc agccacggcc
     1741 ctccatgtca catcaaactg aaaatgaaac cttttttagc aatgaagttt cctgggggat
     1801 gctgtcaaac acctcgctgt acggcggcct gctgctttct ggtgatgact accattctgc
     1861 cgcaatgggt attgggcaaa atatgctgtg gcttggtgcg ctgtcgtttg atgtcacgtg
     1921 ggccagtagc cattttgata ctcagcagga cgagcggggc ttaagctacc gttttaatta
     1981 cagcaaacaa gtggatgcca ctaacagcac gatttccctc gccgcttatc gtttctccga
     2041 tcgtcatttt cacagctacg ccaactatct ggatcacaaa tacaacgaca gcgatgcgca
     2101 ggacgaaaaa cagacgatca gcttatctgt gggccaaccg attaccccac taaacctcaa
     2161 tctttacgcc aacctgctac atcaaacctg gtggaatgca gacgcctcca cgaccgccaa
     2221 catcacggca ggttttaatg ttgatattgg tgactggaga gatatctcta tttcgacgtc
     2281 attcaataca acccattacg aagataaaga tcgcgacaac cagatttacc tgtcgatttc
     2341 gctccccttc ggtaacggtg gtcgggttgg ctatgacatg caaaacagta gccacagcac
     2401 cacacaccgc atgtcgtgga acgatacgct ggatgaacgt aatagctggg gcatgtctgc
     2461 cggactgcaa tccgaccgtc ctgacaatgg agcccaggtg agcggtaact atcagcacct
     2521 gagttcagcg ggtgagtggg atatttctgg tacctatgcc gccaatgatt acagttccgt
     2581 cagcagcagc tggagcggtt ctttcaccgc aacccaatat ggtgcagcgt ttcatcgccg
     2641 cagctccacc aatgaaccac gcctgatggt cagcaccgat ggcgtggcag atattccggt
     2701 tcagggcaat ctcgactaca ccaaccattt tggcattgcg gtggtgccgt tgatttccag
     2761 ttaccagcct tccaccgtgg cggtgaacat gaatgactta cccgacggcg taacagttgc
     2821 agaaaacgtt atcaaagaaa cgtggattga aggcgcgata ggttacaaat cactggcttc
     2881 ccgttccggt aaagacgtta acgtcatcat tcgcaacgcc agcggtcagt ttcctcccct
     2941 cggagcggat atccgccagg atgacagcgg cattagcgtg gggatggttg gcgaggaagg
     3001 acatgcctgg ttaagcggag tcgctgaaaa tcaaaagttt accgtggtct ggggtgatag
     3061 tcagcattgc tcgctccatc ttcctgaaca tatggaagac accgcaaatc gcctgatttt
     3121 accttgtcat taattaagga aatgaaaatg acatttatga aaggactacc ccttttgttg
     3181 ttggttgcca gtctgtgcag ccacgctgca ctacaacccg atcgcactcg tattgtgttt
     3241 aacgctaatg ataaagctac cagtctgcgc gtggataatc gtagcgataa actcccctac
     3301 ctcgcttatt cctggctcga aaatgaaaaa ggggagaaaa gcgatgatct tctggttgcc
     3361 ctgccaccga tacaacgtct ggaaccgaaa gcaacaacgc aggtgcggat agtaaagcaa
     3421 gcctcgaccg cgaaattacc gggcgatcgc gaaacgctat ttttctacaa tatgcgtgaa
     3481 attcctcccg ccccggaaaa aaacagcgac catgccgttc tgcaagtcgc cattcagagt
     3541 cgtatcaagg tgttctggcg accggctgca ttacgcaaga aagcaggaga aaaggtcgaa
     3601 ctgcagttac aggttagcca gcagggcaac caactgacac tgaaaaatcc taccgcgtat
     3661 tatctgacca ttgcctatct ggggagaaat gaaaaaggcg ttttccccgg cttcaaaacc
     3721 gtgatgattg cgccttttag tacggtcaac acaaacaccg gaagttatag cggcagccag
     3781 ttttatctcg gttatatgga tgattacggc gcgttacgca tgacgacgct caactgcagc
     3841 ggagaatgcc gtttacaagc cgtggaggcg aagaaatgag tgctggcaag ggagtgttgc
     3901 tcgtcatctg tctgttattt ctgccgttga agtctgcgct tgccctgaac tgctactttg
     3961 gttcatccgg tggttcggta gaaaaatcag aagtgattca accttttgct gtaccaggca
     4021 atgccaaacc cggcgataag atttgggaat ctgacgatat taaaattccc gtctattgtg
     4081 acaacaatac caacggcaac tttgaaagtg agcatgtcta cgcctgggta aacccctacc
     4141 cgggggtgca ggatcgctat tatcaactcg gcgtaactta caatggcgtt gattatgacg
     4201 ccaaccaggg aaaaagccgc atcgacacta accagtgtat cgacagtaag aacattgaca
     4261 tttatacccc tgagcagatc atcgcgatgg gatggcagaa caaaatttgt tcggacgatc
     4321 ccgctaatat tcacatgtcg cgtacctttc tcgctcgcat gcggttatac gtcaaaatac
     4381 gagaaatgcc gccgcatgat tatcaaagta cgcttagcga ctatatcgtc gtgcaatttg
     4441 acggtgccgg tagcgttaac gaagatccta ccgcccaaaa cctgaaatat catattactg
     4501 gtctggaaaa cattcgcgtg ctggattgca gcgtcaattt ttctatttca ccggaaacac
     4561 aggtgattga ttttggtaaa tttaatttgc tggatattcg gcgacataac atgacgaaaa
     4621 cgttcagtat taaaacgacc aagagccaaa acgatcaatg caccgacgga tttaaagtca
     4681 gttcctcgtt ttatactgaa gaaacgttgg ttgaagaaga taaagcattg ttgattggta
     4741 atggtctgaa gctacgtttg ctggatgaaa acgcctcgcc ttataccttc aataaatatg
     4801 ctgaatacgc cgatttcacc agtgacatgt tgatctacga aaaaacctat acagctgaac
     4861 tttcgtccat tgcaggtacc cccatcgagg ctgggccctt cgatacagtg gtgcttttta
     4921 agattaacta taactgattt gccatgatgc cctttgcagc cggaaagggc atctctttca
     4981 gaaaattgct gattttcccc ttttccttga ttgctgttat tttttcctgg cccggctttc
     5041 tttatgctgt atcgatacac caaagagaaa ataatgaggg agcgaaggat gccagttttg
     5101 cagtggggaa tgttatgtgt gctatcactt ctcctttcta ttggtttcct cgcggtacat
     5161 ctcccggcgg cgctattact tgggccgatg atcgccggga tcatctttag tatgcgcgga
     5221 attactctgc aacttccccg ctctgctttt ctcgccgccc aggccattct tggctgcatg
     5281 attgcgcaaa acctcactgg ttcgattctc accaccctgg ctgtcaactg gccgatcgtg
     5341 cttgcaattt tactggtaac gctgctttcc agcgccattg tgggttggtt attggtgcgc
     5401 tatagctcat tgcctggaaa taccggtgcc tggggctcct ctcctggcgg cgcggcggca
     5461 atggtcgcta tggcgcaaga ttacggcgca gatattcgcc tggtggcgtt tatgcaatat
     5521 ctgcgagtgc tgttcgtcgc gggggccgct gttctggtca cccgcatgat gctgggcgat
     5581 aacgctgaag cggttaacca gcatatcgtc tggttcccgc cagtgagcat taatctcctg
     5641 cttactattt tgctcgcggt cgttgccggt acagtgggat gcctgctgcg tcttccttcc
     5701 ggcacgatgc tcatcccgat gctggcgggc gcagagctcc agtctggtca gctcatcacc
     5761 atcgaactac cggaatggct gctggcgatg gcgtatatgg caattggctg gcggattggt
     5821 cttggtttcg ataaacaaat cttactgcgg gcactacgcc cgctaccgca aatcctgctg
     5881 tcgatttttg ctctgctggc tatttgtgca ggtatggcgt ggggactgac ccggtttatg
     5941 catattgatt ttatgactgc ctacctcgcc accagtcccg gcgggcttga taccgtggcg
     6001 gtcatcgccg cagggagcaa tgccgatatg gcgcttatta tggcgatgca aaccctgcgc
     6061 ctgttcagta ttttgctgac ggggccagcc attgcgcggt ttatttcaac ctatgcgccg
     6121 aagcggtcgg cctagtgctg gcagcctgga caccagtaaa acgggcgcga ggacaacgtg
     6181 gttttctcaa tgatgccgcc acagcgttcg cacagttcgc catcacgatg aaaaacctta
     6241 aagcgaaaca gcgccccatg atgcttattc tcatccacct gcccccgcgt agcgtaagaa
     6301 aaccgaggaa tctccagtaa cgcgtgtgcg agtgcatcca gttgcgccgc attgagatct
     6361 ttcgctttat gatttccagt caacccaacc tgccagagga tctccacccg caaataattg
     6421 ccaagcccag ccagaaacgc ctgatcgagc agtaatcctg caaactgacg gttacgaaag
     6481 cgcggcgaca ataatcgttc ctttaccacc tccggcgtca gattcggatc cagcacatcg
     6541 ggaccaacgc gttgtaaaaa cggatgcgtg gtcagttgtt ccggggtcaa catctcaata
     6601 tccgaggcgc tataaagcag aatggttttg tcaaccgttt gcagttttac ccgcaatact
     6661 cgcgtggtct gcggctcttc gccggtatca accacgcgcc aaacgccgta aagctgatta
     6721 tggctgtaga gcgttaagtc gttggaaaaa tgagttaaca acgccttacc acgcgtttcc
     6781 acatgggtaa cgtgttgacc gataagtggt gattgataag attttaactg cgggaaggca
     6841 aaccagacat cagttagtgg tttgcctttg atcgccgcct ccaggttatc cgctgcacgg
     6901 cggatctccg ggccttcagg catggtgata tccttttttc attctttatt aagcggggtt
     6961 atgctgcgac aacaatccca ttttcggcaa atgtagagcg tagtcggcgg gcgaatgcca
     7021 gtgcgtgctc gccgtcgcca tgcaggcaga ccgtttgcgc cgtgaccgtt gcccattcgc
     7081 cggtgatact tttgactctg ccgtgttgca ccatctccag cgtttgcgcc agcgcctgtt
     7141 cttcgttttc aatcaacgcg cctggctggc ttcgcggcac cagcgagccg tcagcctgat
     7201 aaccgcgatc ggcaaacact tcctcgcgcg ttgtcaggcc atattgcttg cctgcacgaa
     7261 tcagctcgct tcctgccagc ccgacgagaa ccaatgctgg atcgcaagcg tataccgctc
     7321 tggcgatggc ggctgccagt tgtgcttctt tcgccgcctg gttgtacaac atgccgtgcg
     7381 gtttgacatg acgcattacg ccgccttgcg cacgggcaat cgttgccagc gcgccaattt
     7441 gatacagcgt ctgggcgtaa acggtttctg gcggcagctg catggcgctg cgaccaaaat
     7501 tttccctgtc gggaaaactc gggtgagcgc caatcgcgac accatttttt attgcttcac
     7561 gcacgcaagc ctgcatgatt tgcgcatcgc ctgcatgaaa tccacaggca atattggcag
     7621 aggaaaccag cgttaatagc tctgcgtcgc tggcgcagcc ttcgcccaga tcggcgttca
     7681 ggtcaatttt cattgtgcag ccgccacgct aattgttcaa aataacgttg ctgatcttgc
     7741 cgcgctttca gtgcctcttc cagtgaacac tggacaaaat gaatcggctg accgagcgga
     7801 atttgcgcaa gatggtacat atcagcctca atgatgcagg caatacgcgg gtaaccaccg
     7861 gtggtctgtg cgtcgttcat caacacaatc ggctgcccgt tatgtggcac ctgcaccacg
     7921 cccggtaaca aaccgtgaga taacagttcg cgatcggtgg tgcgttttaa aatttgcccc
     7981 tgtaagcgat agcccatgcg gttactttgc gggctaagct gccagggcga acgccagaat
     8041 gcatcctgcg aggcgcgatc gaactcatga tattccggcc ccggcaaggc gcgaatgcgg
     8101 ttgccccaca gcagctgttt aacgccctgc gcttccataa aatcatgctt cgatttgcca
     8161 atcgggagtc ggtcaccatc ctccagtaaa cggccttcca gcccgccaat ccccacgttg
     8221 agatcggtgc tgcatgaccc cattaccggc ggaacatcaa taccacccgc gaccgccaga
     8281 taactgcgca tcccgtgctg cgggcgttta agcgttaaac gctggcctgc tttcatcggc
     8341 aatcgccagc cggtccagac ggcattatca tccagccgcg cttcgcaacc ggcacccgtc
     8401 agagcaaacc acccatcagt ttcgaactca acagttaact gaccgagcgt gatctccagc
     8461 gcgggggcat tggcgtcatt acccaccagt aggttagcaa tgcgtaacgc gggcatatcc
     8521 agtgcgccgc agtggctgat acccgactgg cgaaaaccgt gacgaccgcc atcctgcaca
     8581 gtggtataca tgcccgcacg aataatcttc agcatactcc ctccttctgc ggtacaaagc
     8641 gcacgctgtc tcccggacgt aataagatgg gttcgtcacg cgccggatca aacaggctga
     8701 gtgaggtatg accaatcaac tgccagccac ccggcgttgc cagcggataa acaccagtct
     8761 gcggcccgcc gatcccgaca gaacctgccg gaacgagtaa gcgcggttca gcgcgccgtg
     8821 gcgtgtgtag ttgttccggc aaactcccga gatacgggaa gcccggttga aaacctaaaa
     8881 accagaccac gtattccacg gaggagtgca attcaacaac ctgtttttcg ctcaacccgc
     8941 aatgcgccgc gaccaccgcc aaatccggtc cgcctgcacc accgtaaacc accggaattt
     9001 caataaagcg agactcaggt tccagggcct cgctctcctc ccaccagcgt tgcaaacgct
     9061 ctatggcatc cagcgccagc gactcaggat tacgcaaaat caccgtaata ttgttcatgc
     9121 cgggaatggc ttcaaccaca ttcggcatat ccaccagacg ctgcgccagt cgccagatcc
     9181 gtttctggct agccagcgtc accggcggtt ccagttccag cactaccgcc gtttcaccta
     9241 tcagataaca acgcgctcgt tgcactttcg atccctctga ttattcgtta tgcaggatta
     9301 ggaatatcaa taaaggtcac atcaagatcg gtattttcat tcagccactc gctcaatgcg
     9361 cgaataccac cacgttcagt ggcatggtga cctgcagcat aaaaatgcaa tccctgctcg
     9421 cgggctgaat gaatggtctg ttcagaaact tcgccagtaa taaaagcatc cacgccaaaa
     9481 cgcgcggcgc tatcgataaa actttgcccg ccgcccgtgc accaggcgac gcgctggacc
     9541 acctcaggtc cggtatcgcc acaccataac ggcttacgtc ccagacgcgc ttcaatccag
     9601 gaagccagct ccagtcccgg cacaggcatg gtcagctctc cccacggcac caacggctca
     9661 atttcgccca tgaccgtgat ccccagtaac gccgccagct gtgcgttatt gcccagctca
     9721 ggatgtgcgt caagcggcaa atgccagcca tacaggttga tatcattcgc cagcaacgtt
     9781 tttaaacggt tacgcttcat tccgcgaatg accggagact ctcctttcca gaagtagcca
     9841 tgatgcacaa tgaccgcatc cgcgcccaga cgcactgcct catcgagcaa agcctggctg
     9901 gcggtgacac cggtaacaat tttttgcacc gtctctttgc cttccacctg caaaccattc
     9961 ggcgcgtaat cactaatcgc cgcgctgttc agtttttcgt taatcagttg ttccagttcg
    10021 gtgtttttca tcttcgcgcc ttccgtattc tgatagctac accgttaaca tagcgttacc
    10081 tcgcctgttc gtcgatagcc aatcttttga catattaaga aacaaacccg acgccaaaag
    10141 acgcaaattg tttatatcat cacgcttcag ctaatgtata tttatgcaat cagacagcat
    10201 attgcataaa taatcagatc tgtatcaaag ttttaacttt aaggaaacac aagcacgccg
    10261 cgcaactatt acgtgacgtt gcagtcaatt gtggaattat catgaataaa cacgcatcac
    10321 agccgcgcgc tatttactat gtcgttgcgc tgcaaatctg ggaatatttt agcttttacg
    10381 gcatgcgtgc cctgctgatt ctctatctca ccaatcaact aaaatacaac gatactcacg
    10441 cctacgagtt atttagcgcc tactgttcgc tggtgtatgt cacgccaatc ctcggtggct
    10501 ttttggcgga taaagttctc ggcaatcgca tggcggtgat gctgggggcg ttgttgatgg
    10561 cgatcggtca tgtggtgctg ggtgccagtg agatccatcc gtcattcctc tatctgtccc
    10621 tggcgattat cgtctgcggc tatggcctgt ttaaatctaa cgtaagctgt ctgctcggcg
    10681 agctgtatga gccaaccgat ccgcgtcgtg atggcggttt ctcgctgatg tatgcggcgg
    10741 gtaacgtggg gtctattatc gcacctatcg cctgtggtta cgcccaggaa gagtacagct
    10801 gggcgatggg ctttggcctg gcggcggttg gcatgatcgc gggtctggtc attttcttat
    10861 gtggcaatcg tcatttcact catacccgcg gcgttaacaa aaaagtactg cgtgcgacaa
    10921 actttctcct gccgaactgg ggatggctgc tggttctgct ggtggcaacg cctgcactga
    10981 ttaccgtact gttctggaaa gagtggtcgg tatacgcctt aattgtcgcg actattattg
    11041 gcctgggtgt actggcaaaa atttatcgca aagcagaaaa ccagaaacag cggaaggagc
    11101 tggggctgat tgtgacgctc accttcttca gtatgttgtt ctgggccttc gcacaacagg
    11161 gcggtagctc gattagcctt tatatcgacc gcttcgttaa ccgcgatatg tttggttata
    11221 ccgttccgac cgcgatgttc cagtcgatta atgccttcgc agttatgctg tgcggtgtgt
    11281 tcctggcgtg ggtggtaaaa gagagtgtcg cgggtaatcg taccgtgcgc atctggggga
    11341 aatttgctct tggccttggc ctgatgagcg ccggattctg cattctgacc ttaagcgccc
    11401 gctggtccgc aatgtatggt cactcttctc tgccactgat ggtattaggc ctggcggtga
    11461 tgggctttgc ggaactgttt atcgacccgg ttgccatgtc gcaaattacg cgcattgaaa
    11521 tccccggtgt gaccggcgta ttaaccggca tctacatgct gctttctggc gcgatcgcga
    11581 actatctggc gggcgtgatt gccgatcaga catcgcaggc ttcgtttgat gcttccgggg
    11641 cgatcaacta ctccatcaat gcatatattg aagtatttga tcaaattacc tggggcgcac
    11701 tggcgtgtgt aggagtggta ctaatgattt ggctgtatca ggcgctgaaa ttcagaaacc
    11761 gcgcgctggc gctggagtca taataggcca gcaccatacc tgataaaacg ctcgctgcgt
    11821 tttatcaggc tcctttcctc gcttcttcgt acgctgccag cgttcgcagc cttgcctctt
    11881 tgtgatcaac tatcggttgc ggataatcca gcatcacacc tgctttctgc gcccacttcc
    11941 acggctcatg caccgctttc cctggtacat cgcgcagctc cggtaaccat cgacgaataa
    12001 actcgccctc acggtcaaat ttctcgccct gggttgtcgg gttgaaaata cgaaaatacg
    12061 gcgctgcatc ggttccggtt gaagcggccc actgccagcc accgttattg gctgccaaat
    12121 caccatcaat cagctgcgac atgaaatatc gctcgccttc gcgccagtcg atcaacaaat
    12181 ctttaaccag aaaactggct gtaatcatcc gtagccggtt atgcatccag ccagtgctgt
    12241 taagctgacg catggcagca tcgacaatcg ggtatcccgt tttgccttcc tgccaggcct
    12301 gtaaatgtgc gggattgctc tgccactgca cacgatccgt ccaggcaata aacggacaat
    12361 gtttacacaa cgaggggtaa tacgtcatca gatgacggta gaactcgcgc cagatcagct
    12421 cactaagcca gacactaccg gccccaccgt ccagcgcctg cggctgttcc gccaacaagc
    12481 gatgcaaaca ctggcgaggc gataacccgc ccgttgccag gctggcggac aaacgactgg
    12541 tgccttccac tgccggaaaa tctcgttgtt gctcatattc tccggcaccg ttctggcaaa
    12601 actggcgtaa ttgcgcaatc gccgcttttt cttccaccgg gaaatgcgca gtatcgaaag
    12661 actgacgagg ataattcagc gtaatggatg gcgcgggctc tatcgatccg ctactacgaa
    12721 cttttggcgc agcgacacac tccggcatcc cttcccgcag ccgtttcagc caggcattct
    12781 taaaaggcgt aaagactttg tacatctcgt gattaccggt catcaccgcg ccaggcggca
    12841 ggatcacgct gtcatcaaat ccttcacaca ccacgttacg cagcgctctt tcaacttcca
    12901 catcccgcgc ccgctcattc acttcatact gatagttata aaacaggtgg gtaacgctgt
    12961 tttccgcgca cacctgttta actatttcga cactggcgac aaagtcatcc acttcacgga
    13021 acaataaagg aatacctttt tccgcaagcg ctatttgtag cccattcagt tgagcattga
    13081 taagttcagc ctgacgcggc gacatgttat gcgtcgccca ctggcgtggt gtagcgatat
    13141 ataacgccag cacgcgtgca gacgaattgc ggcaggcggc agccagtgcg agattatcgt
    13201 gcagacgtaa atcctggcga aaccagacca gatgggtaat cataaaactc ctgaatggcg
    13261 caagttaatc acacgcggcc aaagttcgaa ataacgctgc cctgaaagcc aggcgtcagg
    13321 ataaagcgcc atatagtgtt tgatacgcat cagcggcgca agaagtggtt gctcgccacg
    13381 gcgataactg tcgataagcg ccgccaactg ctggcgttct gtggaatcaa tatgcgggcg
    13441 aaaataaccc tgaacgtgca tcaaaacgtt ggtgtgatcg cgcgggttgg cgggatgaga
    13501 aagcaaaaca attacgcgtt ggtagtactg gttgtaaaaa tcatccagat tttgccactg
    13561 gtgaatatca gccacgaacg ggccaagttt gcggtactcc ggctgagaat gcgccagaaa
    13621 gacgagttta tagcggctgt gataatcgag aagcgccccg cgcgttaagc catgttcttt
    13681 cagtcgatgt aactcatgaa gggcgaagat acggattagg gtgctgtcat caaatcgttg
    13741 ctgattcatt actgctcctg aacggtttac aggagtaagt gtagacgttg agaaatgata
    13801 tttgtgaagt taatcctgtt gtagcaggac aaatttacga aagcctgctc cccgcaagga
    13861 ctgacgtcag gtagtttcta accatagctg ccctttctat tcaaacgtga aatcaataag
    13921 ctcatcaggg acatcgcaaa caatatgaag acgggtttct tctctgccgt gactcttttc
    13981 actcattgcg taactgtcat gcttgggatt atttaattct ttcagcggaa atttttcctc
    14041 gaaggcttta tttagccgcc cctggtttcc ttttacagcg aataaataat cacctccctg
    14101 tttttgtatc ttctctgcaa tatctttctg gcaacccatc gcatcggttt tgatgatttt
    14161 tcctttaata tccagcatgt taagaagttc agggatagct gtgatctcat tggatttctt
    14221 atccgtcttg atctgtccga tgaccagact gtgcattgtt gagaacgcac taatgacatg
    14281 aatcgctccc ttacggcgac tcttgtcata agaatgccgg tgtatttttc catcaattgc
    14341 gatgacgtct ttatcatctg aagaatggta gtcaagcatc cagttaataa agctctcatg
    14401 aaatttcgca ggacagatac aggatacaac tctggcaatg gtatcgtgaa caggaatacc
    14461 attttcaaaa tcaccatatt gcttcaaaaa atcgggatgt gtttccccaa aatcctctat
    14521 atcttcccag ccttctgcac cagaaacgac agcgtaaata gtcaacagta gaatatccga
    14581 caatttatgt ttcactttcc aggtttgtct gtaatcggga ataataga
//
LOCUS       DAPHBJ010000105        14477 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.106, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000105 DAPHBJ010000000
VERSION     DAPHBJ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.106"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..893)
                     /locus_tag="RHZ76_002849"
     CDS             complement(<1..893)
                     /locus_tag="RHZ76_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HDV2813458.1"
                     /translation="MKDQITHLPDNADRSVAKQKFKITNWPTYNKALINRGSITFWLD
                     DEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFTLMNVP
                     LRCPDYTSVSKRAKSVNVSFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRI
                     WRKLHLAVDSNTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRAASADGAYDTRLCHD
                     ELRRKKISALIPPRKGAGYWPGEYADRNRAVANQRLTGSNARWKWTTDYNRRSIAETA
                     MYRVKQLFGGSLTLRDYDGQVA"
     gene            complement(943..2418)
                     /gene="silS"
                     /locus_tag="RHZ76_002850"
     CDS             complement(943..2418)
                     /gene="silS"
                     /locus_tag="RHZ76_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005763473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver sensor histidine kinase SilS"
                     /protein_id="HDV2813459.1"
                     /translation="MHSKPSRRPFSLALRLTFFISLSTILAFIAFTWFMLHSVENHFA
                     EQDVSDLQQISTTLNRILQSPVDPDDKKISKIKESIASYRNVALLLLNPRGEVLFSSA
                     QGAALRPAVNSADFSEHSRARDVFLWTVEDPAGPMDTGSEMKMETYRIIASSGQAIFQ
                     GKQQNYVMLTGLSINFHLHYLDALKKNLIAIAVVISLLIVLIIRIAVRQGHLPLRNVS
                     NAIKNITSENLDARLEPTRVPIELEQLVISFNHMIGKIEDVFTRQANFSADIAHEIRT
                     PITNLVTQTEIALSQDRTQRELEDVLYSSLEEYNRMTKMVSDMLFLAQADNNQLIPDR
                     VMFDLRAEVMKVFEFFEAWAEERNITLKFNGMPCLVEGDPQMFRRAINNLLSNALRYT
                     PEGQAITVSIREQESFFDLVIENPGKPIPEEHLSRLFDRFYRVDPSRQRKGEGSGIGL
                     AIVKSIVEAHHGRVQVESDVRSTRFILSVPRLEKMIPETQC"
     gene            complement(2411..3091)
                     /gene="silR"
                     /locus_tag="RHZ76_002851"
     CDS             complement(2411..3091)
                     /gene="silR"
                     /locus_tag="RHZ76_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver response regulator transcription
                     factor SilR"
                     /protein_id="HDV2813460.1"
                     /translation="MKILIVEDEIKTGEYLSKGLTEAGFVVDHADNGLTGYHLAMTAE
                     YDLVILDIMLPDVNGWDIIRMLRTAGKGMPVLLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGNTMITESQFKVADLSIDLVSRKVSRAGNRIVLTSKEFSLLE
                     FFIRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRAKIDNDYGTKLIQTVRGVG
                     YMLEVPDA"
     gene            3281..4666
                     /gene="silC"
                     /locus_tag="RHZ76_002852"
     CDS             3281..4666
                     /gene="silC"
                     /locus_tag="RHZ76_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel SilC"
                     /protein_id="HDV2813461.1"
                     /translation="MFKLKLLSISTIFILAGCVSLAPEYQRPAAPVPQQFSLSHNSLT
                     PAVNGYQDTGWRNFFVDPQVTRLIGEALTNNRDLRMAALKVEEARAQFNVTDADRYPQ
                     LNASSGITYSGGLKGDKPTTQEYDARLELSYELDFFGKLKNMSDADRQNYFASEEARR
                     AVHILLVSNVSQSYFSQQLAYEQLRIARETLKNYEQSYAFVEQQLVTGSTNVLALEQA
                     RGQIESTRAEIAKREGDLAQANNALQLVLGTYRAVPSEKGMKGGEIAPVKLPPNLSSQ
                     ILLQRPDIMEAEYQLKAADANIGAARAAFFPSITLTSGLSSSSTELSSLFTSGSGMWN
                     FIPKIEIPIFNAGRNKANLKLAEIRQQQSVVNYEQKIQSAFKDVSDTLALRDSLSQQL
                     ESQQRYLDSLQITLQRARGLYASGAVSYIEVLDAERSLFATQQTILDLTYSRQVNEIN
                     LFTALGGGWVE"
     gene            4695..5048
                     /gene="cusF"
                     /locus_tag="RHZ76_002853"
     CDS             4695..5048
                     /gene="cusF"
                     /locus_tag="RHZ76_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation efflux system protein CusF"
                     /protein_id="HDV2813462.1"
                     /translation="MRNSLKAVLFGAFSVMFSAGLHAETHQHGDMNAASDASVQQVIK
                     GTGIVKEIDMNSKKITISHEAIPAVGWPAMTMRFTFVNADDAINALKTGNHVDFSFIQ
                     QGNISLLKSINVTQS"
     gene            5162..6454
                     /gene="silB"
                     /locus_tag="RHZ76_002854"
     CDS             5162..6454
                     /gene="silB"
                     /locus_tag="RHZ76_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit SilB"
                     /protein_id="HDV2813463.1"
                     /translation="MASLKIKYAAIIISSLIAGGLISVTAWQYVNSSQKTVQTEQKAP
                     ERKVLFWYDPMKPDTKFDKPGKSPFMDMDLVPKYADESGDKSSGGIRIDPTQVQNLGL
                     KTQKVTRGMLNYSQTIPANVSYNEYQFVIVQARSDGFVEKVYPLTIGDHVKKGTPLID
                     ITIPEWVEAQSEFLLLSGTGGTSTQIKGVLERLRLAGMPEEDIQRLRSTRTIQTRFTI
                     KAPIDGVITAFDLRTGMNISKDKVVAQIQGMDPVWISAAVPESIAYLLKDTSQFEISV
                     PAYPDKTFHVEKWNILPSVDQTTRTLQVRLQVSNKDEFLKPGMNAYLKLNTKSQEMLL
                     IPSQAVIDTGKEQRVITVDDEGKFVPKQIHVLHESQQQSGIGSGLNEGDTVVVSGLFL
                     IDSEANITGALERMRHPEKTESSMPAMSDQPVNMHSGH"
     gene            6465..9611
                     /gene="silA"
                     /locus_tag="RHZ76_002855"
     CDS             6465..9611
                     /gene="silA"
                     /locus_tag="RHZ76_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit SilA"
                     /protein_id="HDV2813464.1"
                     /translation="MIEWIIRRSVANRFLVMMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     LYWARSRVLEYLNQVQGKLPAGVSSEIGPDATGVGWIFEYALVDRNGKHDLSELRSLQ
                     DWFLKFELKTIPNVAEVASVGGVVKQYQIQVNPVKLSQYGISLPEVKQALESSNQEAG
                     GSSVEMAEAEYMVRASGYLQSIDDFNNIVLKTGENGVPVYLRDVARVQTGPEMRRGIA
                     ELNGQGEVAGGVVILRSGKNARDVITAVRDKLETLKASLPEGVEIVTTYDRSQLIDRA
                     IDNLSSKLLEEFIVVAIVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWDHQHPGEQIDNATRWKVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYSMAGAAALAIIVIPILMGFWIRGK
                     IPAETSNPLNRVLIKAYHPLLLRVLHWPKTTLLVAALSIFTVIWPLSQVGGEFLPKIN
                     EGDLLYMPSTLPGVSPAEAAALLQTTDKLIKSVPEVASVFGKTGKAETATDSAPLEMV
                     ETTIQLKPEDQWRPGMTIDKIIDELDRTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLSDIDATAQSIEAVAKTVPGVVSVLAERLEGGRYIDIDINREKASRYGMTV
                     GDVQLFVSSAIGGAMVGETVEGVARYPINIRYPQDYRNSPQALKQMPILTPMKQQITL
                     GDVADINVVSGPTMLKTENARPASWIYVDARGRDMVSVVNDIKTAISEKVKLRPGTSV
                     AFSGQFELLEHANKKLKLMVPMTVMIIFILLYLAFRRVDEALLILMSLPFALVGGIWF
                     LYWQGFHMSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAHPELSRKETFTPEGLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLIWLRRHKKSVS"
     gene            9698..10138
                     /locus_tag="RHZ76_002856"
     CDS             9698..10138
                     /locus_tag="RHZ76_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813465.1"
                     /translation="MKKVVLMALALGLSLPAMASEKVIDMYKSENCGCCSLWGKAMEK
                     DGFEVRTHVMNDQALSALKEKHAIPAGLRSCHTAVAGNLIIEGHVPATTIHKAMQSGS
                     GIYGLATPGMPAGSPGMEMGARKEAYDVIAFSPDGSKKVFQRIE"
     gene            10265..12712
                     /gene="silP"
                     /locus_tag="RHZ76_002857"
     CDS             10265..12712
                     /gene="silP"
                     /locus_tag="RHZ76_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008322814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ag(+)-translocating P-type ATPase SilP"
                     /protein_id="HDV2813466.1"
                     /translation="MKNDNAVQHNNQTASEQTLSPDEGHVLHKVRDPVCGMAILPDRA
                     HSSIRYQDHQLYFCSASCESKFKAHPDRYLTEDASEHSHHHHHDHHEVSPDQIKQPHN
                     QAEKENSEGVWTCPMHPEIRRSGPGSCPVCGMALEPLVATASTGPSDELHDMTRRFWL
                     GLLLAFPVLVLEMGSHLFPDLRNTVPPQYNTWLQLLLASPVVLWCGWPFFARAGMSLR
                     NRSLNMFTLVAMGTGVAWVYSVIATVFPSWFPASFRNMDGLVAVYFEAAAVITVLVLL
                     GQVLELRAREQTSGAITALLNLAPKTARRLDHDGHETDINAEDVLPGDKLRIRPGESI
                     PVDGIVVEGKTTVDESMVTGESMPVTKTEGDPVIGGTINQTGSLIIRAEKVGDETMLS
                     RIVQMVADAQRSRAPIQRMADSVSGWFVPLVILIAVVAFMIWSVWGPEPRMAHGLIAA
                     VSVLIIACPCALGLATPMSIMVGVGKGAQAGVLIKNAEALERLEKVDTLVVDKTGTLT
                     EGSPTVTGIISLNPGGETSLLRVTAAVEKGSQHPLGMAVVKAAQEKGIAIPAVTHFNA
                     PSGKGVSGDVEGQRVVIGNELAMQENSIVIDNQKAVADTLRMEGATVIYVATDGHLAG
                     LIAISDPVKATTPDALKALRQAGIRIVMLTGDNQLTAEAVARKLGIDEVEAGILPDGK
                     KAAITRLKASGHVVAMAGDGVNDAPALAAADVGIAMGTGTDVAIESAGVTLLKGDLMI
                     LNRARHLSEITMKNIRQNLFFAFIYNALGVPVAAGLLYPVYGILLSPVIAAAAMALSS
                     VSVIVNALRLKSVRLGK"
     gene            12753..12950
                     /locus_tag="RHZ76_002858"
     CDS             12753..12950
                     /locus_tag="RHZ76_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HDV2813467.1"
                     /translation="MKSTTYALIAVAAIAAFALLREHWSHVAGYWPYLLLLVCPLMHL
                     FHGHGGHGDHQHQGSENDKKN"
     gene            complement(12984..13721)
                     /locus_tag="RHZ76_002859"
     CDS             complement(12984..13721)
                     /locus_tag="RHZ76_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDV2813468.1"
                     /translation="MYSTDVVKENAYLSATRSGLESNEIATLQRSLPSRFNLRHLKKN
                     ESLKLVLQKKAGKSRVVAYKFTSGSFNYTAYRISDKKFYNLSDTSGKGSLDYPLPATA
                     RLSSPFNPARLNPVSGKVSPHNGIDYSMPMNTKIVSVIDGKITRAEYNSTMGYFVEVT
                     GKAGVKTRYLHLNKILVTKGARVTRGGAIALSGNSGRSSGPHLHYELVINNNPVNSLA
                     FRAAAPADNKLEQHAFAHARDYERYLD"
     gene            complement(14010..14459)
                     /locus_tag="RHZ76_002860"
     CDS             complement(14010..14459)
                     /locus_tag="RHZ76_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004388333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein"
                     /protein_id="HDV2813469.1"
                     /translation="MNILITTTAFTALFCGAAFAQSSDIAHEAHRFVNNASAVSHVNS
                     STHENLPDRVNKNNTPSFSEMNEHERAIVAHSFMNNSASYVHQKMIEEHKKMLSGSDA
                     NSKTSSSSFNELNAGEKAALVHEQVNNAGAEAHQTQARKLRGLYSTR"
BASE COUNT         3493 a         3574 c         3847 g         3563 t
ORIGIN      
        1 gcaacctgac catcgtagtc acgcagtgtc agtgaacctc cgaacagctg ttttacccgg
       61 tacatcgccg tttccgctat cgagcgacgg ttataatctg ttgtccattt ccaccgcgca
      121 ttactcccgg tcagccgctg attcgcaaca gcacggttac ggtctgcata ttcaccgggc
      181 cagtaacccg cgccttttcg gggcgggata agcgcgctga ttttcttacg ccgcagttca
      241 tcgtgacaga gccgggtgtc gtaagcgccg tctgccgatg ctgccctgat ttttctgtga
      301 gtctgccgga taagacccgg gaaggcttct gagtccgtca cattgttcag cgacaggtct
      361 gcacagatga tttcatgtgt gttgctgtca actgccagat gcaactttcg ccagatacgg
      421 cggcgttcct ggccatgttt tttgactttc cattcgcctt caccaaagac cttcagcccg
      481 gtggaatcaa tcaccagatg cgcgatttca ccccgggtgg gcgttttgaa actgacatta
      541 accgactttg cgcgcttgct gacactggtg taatccgggc agcgcaacgg aacattcatc
      601 agtgtaaaaa tggaatcaat aaaaccctgt gcagcccgca gggtcaacct gaacacgcgt
      661 ttaatgacca gaacggtggt gatggcgaga tcagaatagc gctgaggtct tccccgtgat
      721 gaaggcgttg ccgactcata ccaggcctga atagcttcat catccagcca gaaagttatg
      781 gagccacggt tgatgagggc tttattgtag gtgggccagt tggtgatttt gaacttttgc
      841 tttgccacgg aacggtctgc gttgtcggga agatgcgtga tctgatcctt caactcagca
      901 aaagttcgat ttattcaaca aagcccattt acatctttat tttcagcatt gggtttccgg
      961 gatcattttc tccagtctgg gcacggataa gataaaacgc gtggagcgta cgtctgattc
     1021 cacctgcact cttccgtgat gtgcttccac gattgacttc acaatcgcaa ggccgatgcc
     1081 gctgccttct ccttttcgtt gtctggatgg atccacccga taaaaacggt caaacagcct
     1141 tgataaatgt tcttcaggga tcggtttccc cggattttca atcacaaggt caaaaaagct
     1201 ctcctgctct cttattgaga cggtgattgc ctgtccctcc ggggtataac gcagggcatt
     1261 ggataacaga ttattgatcg cccttctgaa catttgtgga tctccctcaa ccaggcaggg
     1321 catcccgtta aatttgagcg tgatattgcg ttcttcggcc caggcttcga aaaactcgaa
     1381 gactttcatg acttccgctc tgaggtcaaa catgaccctg tcaggtatca gctgattatt
     1441 atctgcctgt gccaggaaca gcatatcgct gaccattttg gtcatccggt tatactcttc
     1501 aagactggaa tagaggacat cctcaagttc cctctgtgtt cgatcctgac tcagtgcgat
     1561 ttcagtctgc gtcaccagat tggtgatggg cgttctgatc tcatgcgcga tatcggcaga
     1621 gaaattggcc tggcgggtaa agacatcctc aatctttcca atcatatgat tgaacgagat
     1681 aaccagttgc tccagctcaa tgggaacgcg tgtcggttcc agtcgcgcat caagattctc
     1741 ggaggtgatg tttttaatgg cattgctgac attacgaagg ggcaggtgcc cctgacggac
     1801 agcgattcga atgatcagaa caatcaacag gcttatcacg acggcaatcg caatcaggtt
     1861 ctttttcagc gcatcgaggt aatggagatg gaaattaatg gataggccag tcagcatgac
     1921 atagttctgc tgtttgccct gaaatatcgc ctgaccagag gaggcgataa tcctgtatgt
     1981 ttccatcttc atttcggacc cggtatccat cggtcccgca ggatcctcca ccgtccagag
     2041 aaagacatcc cgtgcgcggc tgtgctcgct aaaatctgct gaattcactg ccgggcgtag
     2101 tgccgccccc tgagctgagc taaagagcac ttcccccctg ggattgagga gcaaaagggc
     2161 aacgttgcgg tagctggcaa ttgattcctt tattttgctt atttttttat catccggatc
     2221 caccggggac tgcagtatac ggttcagtgt ggtgctgatt tgttgaagat cgctgacatc
     2281 ctgctcggca aaatgatttt caacagaatg cagcataaac caggtgaagg cgataaaagc
     2341 cagtatcgtg gacaggctga taaaaaaggt cagccgcaga gcgagtgaga aagggcgtct
     2401 ggaaggtttg ctatgcatcc gggacctcca gcatgtagcc cacgccccgg actgtctgga
     2461 tcagctttgt cccgtaatcg ttgtctattt tagcgcggag tcgctttact gcgacatcga
     2521 tcgcattagt gtcgctgtca aaattcatgt cccagacctg agaggcaatc agggagcggg
     2581 gaagaacctc tccctgatgg cgaatgaaga attccagcag gctgaactct ttactggtga
     2641 gcacaatgcg gtttccggcg cgactgactt ttctggatac gagatcaatc gagaggtcag
     2701 ccaccttaaa ctggctttcc gtgatcatcg tgtttccccg cctcagaagg gttctcaccc
     2761 gggcgagcag ttcagcaaac gcaaagggtt taaccagata atcgtccgca cccagttcca
     2821 gtcctttgac cctatgttcg atcgtgccga gggctgtcag cagtaagacc ggcataccct
     2881 ttccggcagt gcgcagcatg cggatgatat cccagccgtt cacatcaggt agcatgatat
     2941 ccagaatgac taaatcatac tcggctgtca tggcgagatg atatccggta agaccattat
     3001 cagcgtgatc cactacgaac cctgcctctg taagcccttt gctgagatat tcacctgttt
     3061 taatttcgtc ttcgacgatc aatattttca tcttgctccc cggctggctg ctaatgtcat
     3121 tctattgcgc ccacgatcgt tatcaacgga ttacagcaaa aatgacaaca ttgtcattat
     3181 cctgtcaccc ggcaaacaga gagcgttagg taaagtaccc ctatcaatac tctggacttc
     3241 atttgaacca tttaccaggt ctgcctggac gagaagcgtt atgttcaaat taaaattact
     3301 cagcattagc acgatattca tcctggcagg ctgcgtgtcg cttgcgcctg aatatcagcg
     3361 gcccgcagca ccggtacccc agcagttttc actgtcccat aacagcctga cgccagcggt
     3421 aaatggctat caggatacgg gctggcgtaa cttttttgtc gatccccagg ttacccggtt
     3481 gatcggtgaa gctctgacta ataaccgtga tttgagaatg gctgccctga aggttgaaga
     3541 ggcccgagcc cagttcaacg tcacggatgc agatcgttat ccccagctga atgcctcatc
     3601 cgggataaca tacagcggtg gtctgaaagg tgacaagccg accacacagg agtacgacgc
     3661 gagactggag ctcagctatg agctcgattt tttcggcaaa cttaagaaca tgagtgatgc
     3721 tgaccgccag aactactttg ccagcgaaga agcccgtcgg gccgtacaca tcctgctggt
     3781 ctccaacgtt tcacagagct atttcagcca gcaactggcg tacgaacaac tccgtattgc
     3841 gcgggaaacg ctgaaaaatt atgaacagtc ctatgctttc gttgagcaac agctcgtgac
     3901 cgggagtacg aacgttctgg cacttgaaca ggcgagagga caaatcgaaa gtacccgcgc
     3961 cgaaatagcc aaacgagaag gcgatctggc tcaggcaaac aatgccctgc aactggtgct
     4021 tggaacgtac cgcgcagttc cgtcagaaaa agggatgaaa ggtggggaga tcgcaccagt
     4081 aaaattgcca ccaaatctgt cgtcacaaat attgctgcag cgtccggata ttatggaagc
     4141 ggaataccag ttgaaagcgg ctgatgccaa tattggcgca gcgcgagcgg cttttttccc
     4201 gtccattacc ctgaccagtg gtctctcctc aagcagtacg gagctgtcca gcctctttac
     4261 gtcaggaagt ggaatgtgga attttattcc taaaattgaa attcctatat ttaatgctgg
     4321 caggaacaaa gccaatctga agctggctga aattcgccag caacagtcgg tggttaatta
     4381 cgaacaaaaa attcagtcag cctttaagga tgtttccgac acgcttgcgc tgcgcgacag
     4441 ccttagccag caacttgagt cacagcagcg ttatcttgat tcactacaga taactctaca
     4501 gcgtgccaga ggattgtatg caagtggtgc tgtcagttac atcgaagtgc tggatgcaga
     4561 acgttccctc ttcgcgacgc agcaaaccat tctcgatctt acctattccc gacaggttaa
     4621 cgaaattaat ctgtttaccg cgctgggtgg cggttgggta gagtaaattt attcaattaa
     4681 tcaggaaatt aaaaatgcgt aattcactta aagccgtttt atttggtgcc ttctctgtca
     4741 tgttttctgc cggtcttcat gctgaaacac atcagcatgg cgatatgaat gctgccagtg
     4801 atgcttcggt acagcaggtt atcaagggca ccggtatcgt taaagaaatt gatatgaata
     4861 gtaaaaagat caccatttcg cacgaagcaa tccctgcggt gggctggcct gcaatgacca
     4921 tgcgcttcac ttttgttaat gcagacgacg ctatcaatgc cctgaaaacc ggcaaccatg
     4981 tcgatttctc gtttattcag cagggcaata tatccttact caaaagcatt aacgttacgc
     5041 aatcctgatt gactgtccgg agcgaataca tccagtgcgt ctgaacattc attaagggat
     5101 tactgtgaat gaatgatcgg ccgcatatgc caggtgtttt gatttttcag cgagaaattg
     5161 tatggcttct ttaaagataa aatatgctgc aataattatc agcagcctca tagcaggagg
     5221 gctgatatcg gttactgcct ggcagtatgt aaactcatca caaaaaacag tacaaaccga
     5281 acaaaaggca cccgagcgaa aggtactttt ctggtatgac ccgatgaaac cggataccaa
     5341 atttgataaa cccggaaaat ctccctttat ggatatggac ctggtgccaa aatatgctga
     5401 tgaaagcggc gataaaagca gtggcgggat ccgtatcgat ccaacgcagg ttcagaatct
     5461 gggattaaaa acgcaaaaag tcacgcgagg aatgctgaat tattctcaga caatcccggc
     5521 taatgtcagt tacaacgagt atcagtttgt cattgtgcag gcgcgctctg acggtttcgt
     5581 cgaaaaagtg tatcccctga cgattggcga tcatgtgaag aaaggcactc cgcttatcga
     5641 tatcaccatt cctgaatggg ttgaggcaca aagtgagttc ctgctgttat ccggtacagg
     5701 cggtacgtca acccagataa aaggggttct ggagcgactt cgtctggctg gtatgccgga
     5761 agaggatatt caaaggctgc gttcaacccg cacaatccag acccgtttta ccattaaagc
     5821 acctattgat ggtgtcatta ctgcgtttga cctgcgcacc ggcatgaata tttcgaaaga
     5881 taaagtagtg gctcagattc aggggatgga cccggtctgg atcagcgctg cagtgccaga
     5941 atctatcgca tatctgctga aagatacgtc gcagtttgaa atttcggtac cggcttatcc
     6001 ggataaaaca ttccatgtcg aaaaatggaa cattcttccc agcgtggatc agacaacccg
     6061 tacgcttcag gtccgtctcc aggtttctaa taaggatgag tttctcaagc cgggcatgaa
     6121 tgcctatctg aaactgaata ccaagagcca ggagatgctg ctgataccaa gccaggccgt
     6181 tatcgatacc ggcaaagaac agcgcgtgat tactgttgat gatgaaggca agtttgtgcc
     6241 gaaacagatc cacgttctgc atgaatcaca gcaacagtcc ggcatcggtt ccggcctgaa
     6301 tgaaggcgat accgtggtgg tcagtggcct gttcctcatt gactccgaag ccaatattac
     6361 gggcgcgctg gaacgtatgc gccaccctga aaaaacagaa agcagtatgc cagcaatgtc
     6421 tgaccagcct gtaaatatgc attcagggca ctgaggagac gacgatgatt gaatggatta
     6481 tccggcgctc tgtcgccaac cgtttcctgg tcatgatggg ggccctgttt ctcagcatct
     6541 ggggtacatg gacgattatt aacacgccgg tcgatgcctt gcctgacctg tcagatgtgc
     6601 aggtcattat caaaaccagc tatcccggcc aggccccgca gattgtagaa aaccaggtca
     6661 cctatccact taccaccacc atgctgtccg tacctggcgc aaaaaccgtg cgtggttttt
     6721 cacagttcgg ggattcgtat gtgtatgtca tttttgaaga cggcaccgat ctgtactggg
     6781 cccgttcccg cgtgctggaa tatctgaatc aggttcaggg aaaactgcct gccggtgtga
     6841 gttctgaaat cggtcctgat gccacgggtg tgggctggat ttttgaatat gcccttgtcg
     6901 atcgcaacgg aaaacacgac ctttcagaac tgcgctctct gcaggactgg ttcctgaaat
     6961 ttgagctgaa aaccatcccg aacgtggctg aggtcgcttc ggttggcggc gtggtgaaac
     7021 agtaccagat tcaggtcaat ccggtaaaac tgtcccagta cggtatcagc ctgcccgaag
     7081 tgaaacaggc acttgaatcg tctaaccagg aggccggtgg ctcatccgtt gaaatggccg
     7141 aagcggagta tatggtccgt gccagcggtt atcttcagag cattgatgat tttaataaca
     7201 tcgtcctgaa aacaggtgag aacggcgtgc cggtttatct gcgggatgtt gcccgcgtgc
     7261 agaccgggcc cgaaatgagg cgtggtattg ccgagctgaa cggccaggga gaagtcgctg
     7321 gcggcgtggt gatcctgcgg tcgggtaaaa atgcgcgcga cgttatcacg gcagtgaggg
     7381 ataaactgga gacgctgaag gccagcctgc cggaaggcgt tgaaatcgtg accacctacg
     7441 atcgcagcca gttaatcgac cgggcgattg ataacctcag ttccaaactt ctggaagagt
     7501 ttatcgtggt ggccatcgtc tgtgctctgt tcctgtggca cgtacgttct gccctggtgg
     7561 cgattatctc tctgccgctt ggcctgtgta tcgcctttat cgtcatgcac ttccagggac
     7621 tgaacgccaa tatcatgtcg ctgggaggga tagcgattgc cgtcggtgcg atggtggatg
     7681 ccgccattgt gatgattgaa aatgcgcaca aacggcttga ggagtgggat catcagcatc
     7741 cgggtgagca gattgacaac gccacccgct ggaaggtgat taccgacgcc tccgtggaag
     7801 tgggacccgc gttgttcatt agcctgctga tcatcaccct gtcctttatt cctatcttta
     7861 ccctggaagg gcaggaaggt cgtctgtttg gcccgctggc attcacgaaa acgtactcca
     7921 tggcgggagc ggccgcactg gccatcatcg tcattcctat tctgatggga ttctggatcc
     7981 gggggaaaat tcctgccgag accagtaacc ccctgaaccg ggtgctgatc aaagcgtatc
     8041 atcctttgct gctgcgggtc ctccactggc caaaaacaac cctgctggtt gcggccttgt
     8101 ccattttcac ggttatctgg ccactgagtc aggtgggcgg tgaatttctg ccgaagatta
     8161 acgagggcga tctgctgtat atgccgtcga ccttgcctgg cgtctctccg gcagaagctg
     8221 cagcgctcct gcagacaaca gacaagttaa tcaaaagcgt tcctgaagtg gcttctgtat
     8281 ttggcaagac cggtaaagca gagaccgcaa cggattccgc gccgctcgaa atggtggaaa
     8341 ccacgatcca gctcaaacct gaggatcagt ggcgtcccgg catgacaatt gacaagatta
     8401 ttgatgaact cgacaggaca gtccgtttac cgggtctggc aaacctctgg gtgccgccta
     8461 tccgtaaccg tattgatatg ctctcaaccg ggatcaaaag cccgataggt atcaaagtgt
     8521 ccgggactgt tctgtccgat atcgacgcga cggcgcagag tatcgaggcg gtagccaaaa
     8581 ccgtgcctgg cgtggtgtct gtcctggctg agcgacttga gggcgggcgc tacatcgata
     8641 tcgatatcaa ccgggagaaa gcctcccgct acgggatgac ggtaggtgat gtccagctgt
     8701 tcgtctcttc agcaatcgga ggtgctatgg tgggtgagac ggttgaaggg gtggcccggt
     8761 accctattaa cattcgctac ccgcaggatt accggaacag tccgcaggcg ctgaaacaga
     8821 tgccgatcct gaccccgatg aagcagcaga tcacgctggg cgatgtcgcg gatattaacg
     8881 tcgtttctgg accaaccatg ctgaaaaccg aaaatgcccg gccagccagc tggatttatg
     8941 ttgatgcccg cggcagggac atggtgtcgg tggttaacga cattaagacg gccatcagcg
     9001 agaaagtgaa actgagaccg ggaaccagtg tggcattctc cggacagttt gaactgcttg
     9061 agcatgccaa caagaaactg aagctgatgg tgccgatgac ggtgatgatc attttcatcc
     9121 tgttgtatct ggcattccgc cgggttgacg aagccctgct gatcctgatg agcctgccgt
     9181 tcgccctggt tggcggaata tggttcctgt actggcaggg cttccatatg tcagtggcga
     9241 ccggaaccgg gtttatcgcc ctggccgggg tggcagcaga gtttggcgtg gtcatgctga
     9301 tgtatctgcg tcatgccatt gaagcgcacc cggaattgtc ccgtaaagag acgttcacac
     9361 cggaaggtct tgatgaagcc ctctatcatg gtgccgtact gcgtgtccgg ccgaaagcca
     9421 tgaccgtggc ggtgatcatt gcgggtctgc tgccaatact ctggggaacc ggcgcaggtt
     9481 cagaagtcat gagccgtatt gcggcaccca tgattggtgg gatgatcacg gctccgctgc
     9541 tgtccctgtt cattattcct gccgcctaca aattaatctg gctgcgcaga cataaaaaaa
     9601 gcgtgtcctg aacctgaaag ggcaccccct gtgggtgtcc ttctttactg attcaccctg
     9661 acgtcagggt ttatatcgat aatatacaga ggtgagtatg aaaaaagtgg ttctaatggc
     9721 gctggctctc ggcctttcac tacccgcgat ggcgagtgaa aaagtgattg atatgtacaa
     9781 atctgaaaac tgtggctgtt gttccctgtg gggcaaagcg atggaaaaag acgggtttga
     9841 agtacgaact cacgtcatga atgatcaggc gctgtcagcc ctgaaagaaa agcatgctat
     9901 tcctgcagga ctacgaagtt gtcataccgc ggttgccggt aatttgatca ttgaaggcca
     9961 tgtgcctgcg acaacgatac ataaggcaat gcagtctggt tcgggtatat acggtctcgc
    10021 cacccccggt atgccagcag gaagtcctgg aatggagatg ggagcccgaa aagaggctta
    10081 cgatgttatc gcattctcac cggatggaag taaaaaagtc ttccagcgaa tcgaatagtc
    10141 agcggaacgg ctgataacgg gacgccggta gcaggcactc ctgtgccggc gatattcgtg
    10201 gtaatcgcat ccatgacata ccctgaagac agaagatgct tcggtatgca taaggagagt
    10261 tactgtgaaa aatgacaatg cagtgcaaca caacaaccag actgcttctg agcagacatt
    10321 atccccggac gagggccacg tattgcataa ggtgagagat cccgtgtgcg ggatggccat
    10381 cctgcccgac agggcgcaca gcagcattcg ataccaggac caccaacttt atttctgctc
    10441 cgccagctgt gagagtaaat ttaaagccca tcccgatcgt tatcttaccg aagatgccag
    10501 tgaacattcc catcaccatc accacgatca tcacgaagtc agccctgatc agataaaaca
    10561 gcctcacaac caggcggaaa aagagaattc tgaaggtgtg tggacatgtc cgatgcaccc
    10621 ggagatacgc cgcagtggtc ccggaagctg tcctgtctgt ggaatggcac tggagccgct
    10681 cgtagctacg gcatccacgg ggccgagtga tgaacttcac gacatgacaa gacgcttctg
    10741 gctggggttg ttgctggcgt ttccggttct ggtactcgaa atgggatctc atctgtttcc
    10801 cgacttgagg aatacagtac cgccacagta caacacatgg ctgcagttgc ttctggcctc
    10861 ccctgtcgtg ttgtggtgtg gctggccatt cttcgcccgg gccggaatgt cgttacgtaa
    10921 ccgctccctg aatatgttta cccttgttgc aatggggacc ggcgtagcct gggtttacag
    10981 cgtcattgca accgtcttcc cctcctggtt tcctgcatcg ttcagaaaca tggatggcct
    11041 ggtggccgtt tattttgaag ccgcagcagt tattacggtg cttgttctgc tgggacaggt
    11101 tcttgagctg cgggcacggg aacaaacctc aggcgccatt actgcgcttc tgaaccttgc
    11161 ccccaaaacc gccagacggc tggatcatga cggtcatgaa acggatatta atgcggaaga
    11221 tgtcctgcct ggcgataagc tccgcatcag acctggagag agtattccgg tcgacggtat
    11281 cgtggtcgaa ggcaaaacaa ccgttgatga atcgatggtg accggggaat ctatgcctgt
    11341 taccaaaacg gagggtgacc ctgtcatcgg ggggaccatt aatcagacag gtagtcttat
    11401 catccgtgca gagaaagtcg gtgatgaaac gatgctctca cgaattgttc agatggtcgc
    11461 tgatgcacag cgttcgcggg cccccatcca gagaatggct gacagcgttt caggctggtt
    11521 tgttcctctg gtgatactta tcgcggttgt tgctttcatg atctggtctg tctgggggcc
    11581 cgagcccagg atggcgcacg gtctcattgc ggctgtgtcg gtcctgatta ttgcctgtcc
    11641 ctgcgcgctg gggctggcca cgccgatgtc gataatggtg ggggtgggca aaggagccca
    11701 ggccggggtg ttaatcaaga atgccgaagc ccttgagcgt cttgaaaaag tggacacgct
    11761 ggttgtcgac aaaacaggca cgctcacgga aggttcgccg acggtgacag ggattatcag
    11821 tctcaatccg ggtggggaaa catctctttt gcgtgtaacg gccgcagtgg aaaaaggctc
    11881 gcagcatccg ctgggtatgg cagtagttaa agcagcacag gaaaagggga tcgcaatacc
    11941 cgcagtcact catttcaatg cgccgtcggg taaaggtgtc tcaggcgatg tcgaaggtca
    12001 acgggttgtt attggtaatg aactggctat gcaggaaaac agtatcgtta ttgataatca
    12061 aaaggccgtt gcggatacgt tgcggatgga aggtgctacc gttatctatg tggccacaga
    12121 cgggcacctt gcaggcctga tagctatctc ggatcccgtg aaagcaacca cgccggatgc
    12181 gcttaaagct ttgcgtcagg cggggatccg catcgttatg ctcaccgggg ataaccagct
    12241 taccgctgaa gcagtcgcac ggaaactggg aatagatgag gttgaagccg ggattctgcc
    12301 ggatggcaaa aaagcagcga taacccgact gaaagcgtct ggccatgtgg ttgcgatggc
    12361 cggagacggt gtgaatgatg ccccggcgct ggcagcggct gacgtgggaa tagccatggg
    12421 aacgggtaca gatgtggcaa ttgaaagtgc cggagtcacc cttctcaaag gcgacttgat
    12481 gatactgaac agggcccgtc atctgtcaga aatcaccatg aaaaatatcc gacagaatct
    12541 gttttttgca tttatctaca acgcacttgg cgtgcctgtg gctgcaggcc tgctttatcc
    12601 tgtgtatgga atactgctgt cgccagttat tgcggcggcg gccatggctc tttcctccgt
    12661 cagcgtcatt gtgaatgcgt tgcgtctgaa aagtgtcagg ctcgggaaat aacactgagt
    12721 gaaggggctg ttacgaacag aaggagtcca gtatgaaaag taccacctat gcgcttattg
    12781 ctgtcgccgc gatcgcggca tttgccctcc tgcgcgaaca ctggtcacat gtggcaggtt
    12841 actggccata tctgttattg ctggtctgcc cgctaatgca tcttttccac ggccacggag
    12901 ggcatggaga tcatcaacat caaggaagtg aaaacgataa aaaaaattaa tccggcagac
    12961 ggggccgcgt cgcggccccg ttatcagtcc aggtatcgtt cgtagtctct ggcatgcgca
    13021 aaggcatgct gttcaagttt gttatcagcg ggtgccgctg cccggaacgc cagtgagtta
    13081 acaggattgt tattgatgac cagctcgtaa tgcagatgag gaccggatga acgtccgctg
    13141 ttaccggata acgcaatagc gcctccccgg gtaaccctgg cccctttagt aacgagtatt
    13201 ttattgaggt ggagatagcg agttttaaca ccggcttttc ctgttacttc aacaaaatat
    13261 cccatggtgc tgttgtattc ggcccgggtg atttttccgt cgatgacgct gactattttc
    13321 gtgttcatgg gcatggaata atcaatgcca ttatggggac tcacttttcc cgataccggg
    13381 ttaagtcttg caggattgaa aggcgaactg agtcttgctg tggccggtaa cggataatcg
    13441 agactgcctt tcccggaagt atcggaaagg ttatagaact ttttatctga tatacgatac
    13501 gccgtgtaat taaatgaacc ggacgtaaat ttataggcca cgacacgtga ttttcccgct
    13561 ttcttttgca gtacgagttt taatgattca ttttttttca aatgccgcag attaaaccgg
    13621 gaaggcaagg agcgctgaag agtagcgatc tcgttcgatt ccagccccga gcgggtggct
    13681 gaaaggtagg cattttcttt tacgacatcg gtagaataca tttactggaa ttcgccgtta
    13741 gcatgaacac tgcggcgtat gctaggggtt ttcaggaggc gtgtcatctg tcagttaatc
    13801 gggagcaccg ttgatggtcg tcatttttgt aacatatctt gtttcccgtt tgctcctgaa
    13861 gctctgggaa ctgtatgacc agcccgacgg tgatgattaa cgggactgaa acaggttacg
    13921 gcagagcaat atggggctca tgtcctgcta cgtaacccgt cagtaaagcc ctgctgcgca
    13981 cctgacgcta agcactaacc cgcctgcagt tacctggtcg aatacagccc gcgaagcttt
    14041 cttgcctgcg tctgatgtgc ttccgcaccg gcattattga cctgctcatg cacgagagcg
    14101 gctttttctc cggcattcag ttcgttaaaa gaagaagacg aggtctttga atttgcatca
    14161 ctgccggaca gcattttttt atgttcctca atcattttct gatgcacata ggacgcgctg
    14221 ttgttcataa atgaatgagc aacaatggcc ctttcatgtt cattcatttc agagaatgag
    14281 ggcgtgttgt ttttattaac cctgtccggt aagttttcat gcgtcgagga gttcacatga
    14341 ctgacggctg aggcattatt aacaaatcga tgtgcttcat gggcaatatc actggactga
    14401 gcaaaagctg ccccacaaaa taaagctgta aacgcagtgg tcgtgattaa tatattcatg
    14461 tgtaattacc ttctgag
//
LOCUS       DAPHBJ010000106        14346 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.107, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000106 DAPHBJ010000000
VERSION     DAPHBJ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.107"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            166..1224
                     /gene="citC"
                     /locus_tag="RHZ76_002861"
     CDS             166..1224
                     /gene="citC"
                     /locus_tag="RHZ76_002861"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HDV2813470.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1239..1535
                     /gene="citD"
                     /locus_tag="RHZ76_002862"
     CDS             1239..1535
                     /gene="citD"
                     /locus_tag="RHZ76_002862"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HDV2813471.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1532..2440
                     /gene="citE"
                     /locus_tag="RHZ76_002863"
     CDS             1532..2440
                     /gene="citE"
                     /locus_tag="RHZ76_002863"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HDV2813472.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2451..3983
                     /gene="citF"
                     /locus_tag="RHZ76_002864"
     CDS             2451..3983
                     /gene="citF"
                     /locus_tag="RHZ76_002864"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HDV2813473.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3987..4538
                     /gene="citX"
                     /locus_tag="RHZ76_002865"
     CDS             3987..4538
                     /gene="citX"
                     /locus_tag="RHZ76_002865"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HDV2813474.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDCEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPLRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4513..5391
                     /gene="citG"
                     /locus_tag="RHZ76_002866"
     CDS             4513..5391
                     /gene="citG"
                     /locus_tag="RHZ76_002866"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HDV2813475.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5442..6905
                     /gene="citT"
                     /locus_tag="RHZ76_002867"
     CDS             5442..6905
                     /gene="citT"
                     /locus_tag="RHZ76_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HDV2813476.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7019..7825
                     /gene="rna"
                     /locus_tag="RHZ76_002868"
     CDS             7019..7825
                     /gene="rna"
                     /locus_tag="RHZ76_002868"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HDV2813477.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKTVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8054..8464
                     /gene="rnk"
                     /locus_tag="RHZ76_002869"
     CDS             8054..8464
                     /gene="rnk"
                     /locus_tag="RHZ76_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HDV2813478.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8695..9933)
                     /locus_tag="RHZ76_002870"
     CDS             complement(8695..9933)
                     /locus_tag="RHZ76_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HDV2813479.1"
                     /translation="MKALTYHGPHHVQVENVPDPGVEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLHFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLSFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAGRGYEIFEKREEECRKVILVPGAQN
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10154..10582
                     /gene="uspG"
                     /locus_tag="RHZ76_002871"
     CDS             10154..10582
                     /gene="uspG"
                     /locus_tag="RHZ76_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HDV2813480.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10703..12268)
                     /gene="ahpF"
                     /locus_tag="RHZ76_002872"
     CDS             complement(10703..12268)
                     /gene="ahpF"
                     /locus_tag="RHZ76_002872"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDV2813481.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12513..13076)
                     /gene="ahpC"
                     /locus_tag="RHZ76_002873"
     CDS             complement(12513..13076)
                     /gene="ahpC"
                     /locus_tag="RHZ76_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HDV2813482.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13448..14194
                     /gene="dsbG"
                     /locus_tag="RHZ76_002874"
     CDS             13448..14194
                     /gene="dsbG"
                     /locus_tag="RHZ76_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HDV2813483.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3419 a         3793 c         3608 g         3526 t
ORIGIN      
        1 atttcacgcc gaaatactcc ttaggatgta tagcgaaagg agaaaaagat atacctcgat
       61 cacccacctt tctcccaagt gaaaataaaa agttatcagt ttgcaacatt gaacaacatt
      121 cgttgcaaat cgataacaac atgcaccttc aggatactat ttattatgtt cggcaatgat
      181 attttcaccc gcgtaaaacg ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg
      241 catgaaaatg atttgagcgt tgacaccaca gtcgaagtat ttattaccgt aacccgcgat
      301 gaaaagctta tcgcgtgcgg tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt
      361 gaatccgttc gcggtgaagg actggcgctg acattagcca ctgaactgat aaacctcgcc
      421 tatgagcggc acagcacgca tctgtttatt tataccaaaa ccgaatacga ggcgctgttc
      481 cgccagtgcg gtttttccac gctgaccagc gtacccggcg tgatggtgct gatggaaaac
      541 agcgccacgc gactgaaacg ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac
      601 aagattggct gcattgtgat gaacgccaat ccctttacga atggtcaccg ttatctgatt
      661 caacaggctg cggcacagtg cgactggttg catctgtttt tagtcaaaga agattcttca
      721 cgcttcccct atgaagaccg gctggatctg gtgttaaaag gcaccgccga tattccacgc
      781 ctgactgtgc atcgcggctc cgaatacatc atctcccgcg ctacgttccc ttgctacttc
      841 attaaagaac agagcgtcat taaccattgt tacaccgaaa ttgatctgaa gattttccgt
      901 cagtacctcg ctcccgcact gggtgtaact caccgctttg tcggtactga acccttttgt
      961 cgcgttaccg cccagtacaa ccaggatatg cgctactggc tggaaacgcc gactatctcc
     1021 gcaccgccca tcgaactggt tgaaattgag cggctgcgtt accaggagat gccgatatcc
     1081 gcttcccggg tacgtcaact gctggcgaaa aacgatctca cggctatcgc gccgctggtc
     1141 cctgcagtca cgctgcatta tttgcagaac ctgcttgagc actcccgcca ggacgcggca
     1201 gctcgtcaaa agacccccgc atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg
     1261 ttgcaggcac ccttgagtct ggggatgtga tgatacgcat cgccccactc gatacgcagg
     1321 atatcgacct gcaaatcaac agcagcgttg agaaacagtt tggcgatgca attcgcacca
     1381 ccattctgga cgttctcgcc cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca
     1441 aaggcgcact ggactgcatt ttacgtgcac gactggaagc cctgctggca cgcgccagcg
     1501 gtatcccggc tctgccatgg gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa
     1561 aactcgcacc cgccgcagca tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa
     1621 ctccttcatc tacccggctg atgccctgat gtttgacctc gaagactccg tagcattgcg
     1681 tgaaaaagac accgcccgcc gcatggttta ccacgcgctg caacatccgc tgtatcgcga
     1741 tattgaaacc attgtgcgtg tcaacgcgct ggattccgaa tggggtgtca acgacctgga
     1801 agccgtcgtt cgcggtggtg cggacgttgt gcgtctgccg aaaaccgata ccgctcagga
     1861 tgttctggat atcgaaaaag agatcctgcg tatcgaaaaa gcctgtggtc gtgaacccgg
     1921 cagcaccggc ctgctggcgg cgattgaatc tccgctgggc attacccgcg cagtggaaat
     1981 cgctcacgct tccgagcgtt tgatcggtat cgccctcggt gcagaagact atgtgcgcaa
     2041 cctgcgtaca gaacgctccc cggaaggaac tgaactgctg ttcgctcgct gctccatttt
     2101 gcaggccgcg cgctctgcgg gtattcaggc gttcgatacc gtctattccg acgctaacaa
     2161 cgaagctgga ttcctgcaag aagccgccca catcaaacag ctgggctttg acggcaaatc
     2221 gctgatcaac ccgcgtcaga ttgatctgct gcacaacctc tacgcaccga cccagaaaga
     2281 agtggatcac gcccgccgcg tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg
     2341 cgtggtttcc ctgaacggca agatggtgga cggtccggtt atcgatcgcg cccgtctggt
     2401 gctctcccgt gcagaacttt ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga
     2461 aaattgaaca atctcaacga caagaacggg tagcggcctg gaatcgtcgc gctgaatgcg
     2521 atcttgccgc tttccagaac tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc
     2581 gcaaactgtg cgccaacctg gaagaagcga ttcgtcgctc tggtttacag gacggcatga
     2641 cggtttcctt ccatcacgct ttccgtggcg gtgacctgac cgtcaatatg gtgatggacg
     2701 tcatcgcgaa gatgggcttt aaaaacctga ccctggcgtc cagctccctg agtgattgcc
     2761 atgcgccgct ggtagaacac attcgccagg gcgtggttac ccgcatttat acctccggcc
     2821 tgcgtggtcc actggcggaa gagatctccc gtggtctgct ggcggaaccg gtgcagatcc
     2881 actctcacgg cggacgtgtg catctggtac agagcggcga actgaatatc gacgtggctt
     2941 tcctcggcgt cccgtcctgt gatgaattcg gtaatgccaa cggctacacc ggtaaagcct
     3001 gctgcggctc cctcggctat gcaatggttg atgccgacaa cgcaaaacag gttgtgatgc
     3061 ttaccgaaga actgctgcct tatccgcata atccggcaag cattgagcaa gatcaggttg
     3121 atctgatcgt caaagttgat cgcgttggcg atgctgcaaa aatcggcgct ggcgctaccc
     3181 gtatgaccac taacccgcgc gaactgctta ttgcccgtag cgctgcggat gtgattgtca
     3241 actctggcta cttcaaagaa ggtttctcca tgcaaaccgg caccggcggc gcatcgctgg
     3301 cggtaacccg tttcctggaa gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc
     3361 ttggcggtat taccgcgacg atggttgacc tgcacgaaaa aggtctgatc cgcaaactgc
     3421 tggatgtgca gagctttgac agccatgctg cgcaatcgct ggcccgtaac cccaatcaca
     3481 tcgaaatcag cgccaaccag tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc
     3541 tcgacgtggt ggtactgagc gcgctggaaa ttgacaccca gttcaacgtt aacgtgctga
     3601 ccggctctga cggcgtactg cgtggtgctt ccggtggtca ctgcgatacc gcaattgcct
     3661 ctgcgctttc catcatcgtc gcgccgctgg tacgcggtcg tattccgact ctggtggata
     3721 acgtgctgac ctgcatcacc ccgggctcca gtgtcgatat tctggtcaca gaccacggta
     3781 tcgcagttaa cccggcacgt ccggaactgg cagaacgtct gcaggaagcg ggcattaaag
     3841 tggtttccat tgagtggctg cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga
     3901 ttgaattcac cgaccgcgtc gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg
     3961 ttgtgcatca ggtgaaggaa taagccatgc acctgcttcc tgaactcgcc agccaccatg
     4021 cggtatcaat tcccgagctg ctcgtcagcc gggatgaaag gcaagcacgg caacacgtct
     4081 ggctcaagcg ccatcctgtt ccactggtct cctttaccgt ggttgcgcct gggccgatta
     4141 aagactgcga ggtcacacgc cgaattttta atcatggcgt gacagccttg cgtgccttag
     4201 ccgcaaaaca gggctggcaa attcaggagc aggctgcact ggtttccgcc agcgggccgg
     4261 agggcatgtt gagcattgcc gccccggctc gcgacctcaa gctcgccacc attgagcttg
     4321 aacatagtca tcctctcggg cggttatggg atatcgatgt cctgacgccc gaaggcgaaa
     4381 ttctctcccg ccgcgactat tcactgccgc ttcgccgctg cctgttgtgc gagcaaagcg
     4441 cagccgtctg cgcgcgtgga aaaacccatc aactgaccga tttactcaac cgcatggagg
     4501 cactgctgaa cgatgtcgat gcctgcaacg tcaactaaaa ccacaaagct tgcgacgtca
     4561 ttaatcgatg agtacgccct gctgggctgg cgcgccatgc tgactgaagt caatctgtca
     4621 ccgaaaccag gcctcgtgga tcgcattaac tgcggtgcgc acaaagatat ggcgctggaa
     4681 gatttccacc gcagcgcgct ggcgattcag ggctggctac cccgtttcat tgaatttggt
     4741 gcctgtagtg cggaaatggc accagaagcg gtactccacg gattacgccc aattggtatg
     4801 gcttgcgaag gtgatatgtt ccgcgccact gcgggcgtaa acacgcataa aggcagcatt
     4861 ttttctttag ggctgctatg tgcggcaatt ggccgtttgc ttcaactcaa ccaaccggta
     4921 acgccaacaa ccgtttgttc tacggcggca agtttctgcc gtggcctgac cgatcgcgaa
     4981 ctgcgtacca ataattcaca actgacggca ggtcaacggt tgtaccaaca gcttggcctt
     5041 accggcgcac gcggtgaagc cgaagcgggt tatccactgg tgatcaatca cgccttgccg
     5101 cattacctca ctctgctgga tcaggggtta gatcctgaac tggcattgct cgataccttg
     5161 ctcctactga tggcgatcaa cggcgatacc aacgttgcat cgcgcggtgg cgaggggggc
     5221 ctgcgctggc tacagcgcga ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc
     5281 gccgatctcg attatctccg gcagttcgac agggagtgta tcgaacgaaa tctcagtcca
     5341 ggcggcagtg ccgacctgct gatccttacc tggtttttag cacagattta attatttaag
     5401 cacttgataa atttggaaat attaattttc ggagaacccg tatgtcttta gcaaaagata
     5461 atatatggaa actattggcc ccactggtgg tgatgggtgt catgtttctt atccctgtcc
     5521 ccgacggtat gccaccacag gcatggcatt acttcgctgt gtttgtggca atgattgtcg
     5581 gcatgatcct cgagccaatt ccggcaacag cgatcagttt tattgcggtt actatttgcg
     5641 ttattggcag taattacctg ctctttgatg ccaaagaatt agctgaccca gcgtttaatg
     5701 cgcaaaaaca ggcgctgaaa tggggtctgg ctggtttttc cagcaccacg gtatggctgg
     5761 tatttggcgc atttattttt gcattagggt atgaagtttc cgggttaggt cgtcgcattg
     5821 cccttttcct ggtgaaattc atgggcaaac gcacgctgac gttgggttat gcgattgtca
     5881 ttatcgacat tctgctggca ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg
     5941 tttttccggt cattaaaaac ctgccgccgc tgtttaaatc attccccaac gatccgtccg
     6001 cgcgtcgtat tggcggctat ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt
     6061 ccatgtttgt caccggtgcg gcaccaaacg tgctgggtct ggagttcgtc agcaaaattg
     6121 ccggtatcca gattagctgg ttgcagtggt tcctctgctt cctgccggtt ggggttatct
     6181 tgcttatcat tgcgccgtgg ctttcctacg tgctgtacaa accggaaatc acacacagtg
     6241 aagaagtggc aacctgggcg ggtgatgaac taaaaaccat gggtgcgctg acacgcagag
     6301 agtggacgct gattggcctt gtattgctca gcttaggttt gtgggtattt ggcagtgaag
     6361 tcattaatgc tactgcggtt ggtctgctgg cagtttcgct aatgctggct ctgcacgttg
     6421 tgccgtggaa agacattacc cgctataaca gcgcatggaa cacgctggtc aacctggcaa
     6481 ctctggttgt gatggctaac ggcctgactc gttctggttt tattgactgg ttcgccggta
     6541 ccatgagtac gcacctggaa ggattctcac caaacgcaac ggtgattgta ctggttctgg
     6601 tgttctactt tgcacactac ctgtttgcca gcctgtctgc gcacaccgca accatgctgc
     6661 cggttattct ggccgtcggt aaaggtattc cgggcgtacc aatggaacaa ctgtgtatcc
     6721 tgctggtgct gtctatcggt atcatgggct gtctgacgcc gtatgcaacc ggtcctgggg
     6781 tgattattta cggctgtggc tatgtgaaat caaaagatta ctggcgtctt ggcgcaatct
     6841 tcggggtgat ttacatctct atgttgctgt tggttggctg gccgattctc gccatgtgga
     6901 actaatacgt tcgctaaacg ctgaataaat catcacgccc gccaggtaag cccacctagc
     6961 gggctttttt atgatttaat agatagtatt cagtgtatgt atgagttcca cacccattat
     7021 gaaagcattc tggcgtaacg ccgcgttgct cgcggtttct ctgcttccct tctcttctgc
     7081 caacgccgta gcgttgcagg caaaacagta tggcgatttt gatcgctatg tcctggccct
     7141 ctcctggcaa accggatttt gccagagtca acacgatcga aatcgtaacg aacgagatga
     7201 atgtcgcctg caaaccgaaa cgaccaacaa agctgatttt ctgaccgtac atggtctgtg
     7261 gccaggattg cctaaatcgg tcgctgcccg tggtgttgat gaacgccgct ggatgcgctt
     7321 cggttgcgct actcgcccaa tcccgaatct accagaagcg cgcgccagcc ggatgtgttc
     7381 atcgccggaa accggattat cactggaaac ggccgctaaa ctaagtgaag tcatgccagg
     7441 agctggcgga cgttcctgcc tggaacgcta cgaatatgcc aaacacggtg cctgctttgg
     7501 ttttgatccg gacgcatact tcggtactat ggtacgcctg aatcaagaaa ttaaagagag
     7561 cgaagccgga aaattccttg cggataatta cggtaaaaca gtgagccgcc gtgactttga
     7621 cgccgccttt gccaaaagct ggggaaaaga gaacgtgaaa acagttaagc taacgtgcca
     7681 gggtaaccct gcgtatttga ctgaaattca gatctcgatc aaagctgacg ccatcaacgc
     7741 tccgctttct gcaaactcat tcttgccaca acctcaccca ggtaactgtg gcaaaacctt
     7801 tgtgattgat aaagcgggtt attaatcctc tgatttacaa aacacgctaa ttcgtcttcc
     7861 ataaccatga cgattaagaa ttatacataa tgcgctgcat tgaggaattc cctgaatgtg
     7921 acgcaaatca cttcaaagag tgagcaacag tcagtatcat catgtgagtt aaaccctcgc
     7981 cgcctgacgg tgagggtttt cttttgggta tgtttatctg cgacactcgc agtaccgacg
     8041 acatggagta aaaatgtcca gaccaactat catcattaac gacctggatg ccgaacgcat
     8101 cgatattctg ctggagcaac ccgcctatgc tggtttgcca atcgccgacg cgttaaacgc
     8161 agagttggat cgcgcccaaa tgtgttcgcc agaagagatg ccacacgacg tggtgacaat
     8221 gaacagccgg gttaaattcc gcaatcttag cgatggcgaa gtgcgtgtgc gcacgctggt
     8281 gtatccggca aaaatgaccg atagcaatac tcagctttcc gttatggctc cggtaggtgc
     8341 cgcactgctg gggctgcgcg ttggcgattc cattcactgg gaacttccgg gcggcgttgc
     8401 aacccacctt gaagtgctgg aactcgaata ccagccagaa gctgctggcg actacctgct
     8461 ttaatcccat ctgcacaagc gataactcgc tttgcccaga ggatgcactc gcatcctctt
     8521 tacgcaaaat cagcaatttt tcctggttct cactgttcac ttttgctgca aaattattcc
     8581 gtttcgtttg tgggaataac catggcagaa gcattttaca tcctgatagg atttctgatt
     8641 atggcggcga ttatcgtcat ggctgtcctt tacctcgaaa accactcctg acgatcatat
     8701 tgttcccccc ggcatcgcat tcaccagacc tgaaaccgcc ttctgcgccg cctctgcgtt
     8761 ttgtgcaccg ggtaccagaa tcaccttacg gcactcctct tcacgttttt cgaaaatctc
     8821 atatccccgg ccggcctctt caaacggcat atagtgggta acaatttctt ctggtttcag
     8881 taatcctttc tcaattaacg gtaataattc tcccagccat gcgtgaacgt gggtctgtcc
     8941 cattttaaac gacaaccctt tatcaaaggc gtcgccaaac aggaaaccgt gaataaatcc
     9001 agcgtagacg cccggtacgc taacaatgcc gccacgcctg accgccgcaa tacactgacg
     9061 caacgcttta ccgctgctgc cctccagttt caggttagtc agcaccgttt ccgtggtgct
     9121 gcctttcgct tcaaaaccga cggcgtctat tactgcatcc acgccccggt gacctgccgt
     9181 ttgttcaata attgactgtg ccggatcgct gtcttcatca aaattaatcg ggatcgcgcc
     9241 gtagcggtcg gcggcgaaat gcaagcggta gggatgatga tcaacaacaa aaatctgttc
     9301 cgcaccgagc aaccgtgcac aggcgattgt caacaatccc acaggaccag caccatagac
     9361 tgcaacgctt gaaccttgtt ggatctgcgc attttttgct gcctgccatg ccgttggcag
     9421 aatatcagaa aggaaaagcg ctttatcatc tgaaagcaaa ggcggtactt taaacggccc
     9481 cacattccct ttagggacgc ggacatattc cgcctgccca ccaggaacgc cgccatacag
     9541 gtgactataa ccaaacaatg ccgctggagc tggtatctgt tttttattga gcgcagcgcc
     9601 tttacccgca ttggtatttt cgcaggcggc atattgttgc aatcgacaga aaaaacagtc
     9661 gccacaagca atgacgaacg gaattaccac tcggtcgcct ttttgcaaat tttttacgtc
     9721 ctttccggtt tcaactactt cccccataaa ttcatgacca aaaatatcgc catgtttaac
     9781 ctgaggtatt ttgcctcgat aaagatggag gtcagagcca cagatcgccg ttgccgtaat
     9841 acgcagaata atatcatctg cctgttcaac gcccggatcg ggaacatttt ctacctgaac
     9901 gtgatgtggg ccgtgataag tcaatgcttt catgcaacct ccagagacag ttcaaaaaag
     9961 agtcagtctt taagggtagc tatcacagcg ggaaaacggg cggtaccgca ggttttggga
    10021 gaattcctgg tgttcagtga gaatatatta atcacagaaa ccgttcgtaa ggaaaaagaa
    10081 ccagacaaat atcagggata aatattataa tcatggcgaa tgatttgttc atgattaaca
    10141 gggagaaagg gttatgtata agacaatcat tatgccagtt gatgtatttg aaatggaatt
    10201 gagcgacaaa gctgttcgcc acgctgaatt cctcgcccag gatgacggag ttattcatct
    10261 acttcacgta ctacccgggt cagccagcct gagcctgcac cgttttgccg ctgatgtgcg
    10321 tcgttttgaa gagcatctgc aacatgaagc agaagaacgt ctgcaaacga tggtcagcca
    10381 cttcaccatc gatccttccc gcattaaaca acatgtccgt tttggtagcg tgcgggatga
    10441 agtcaatgag ttggcagaag aactgggggc tgatgttgta gttattggtt ctcgcaaccc
    10501 atcgatttcg acccatctgt taggttctaa cgcctcgagc gtgatccgcc atgccaatct
    10561 gccggtgctg gttgtgcgtt aattgccaga ataaatatcc cgccctgccc ggagccatcc
    10621 cggcagggct ttttaatgcc ataaaaaagc cgccaggttt gaccccggcg gctaagcaat
    10681 tgcaggtgaa tcttacttct tcttatgcag ttttggtgcg aatcaggtag tcaaaagcac
    10741 tcagagaggc tttggcacct tcgccagtgg cgatgatgat ctgcttgtac ggaaccgtcg
    10801 tacagtcacc cgctgcgaat acgcctttga cgttggtttc gcatttcgca tcaatgataa
    10861 tctcacccat gcggttacgt tcgactgcgc cttccagcca gttggtgttc ggcagcagac
    10921 caatctggac gaaaataccg gccagttcga tgttgtgaat atcgccgctg acacgatctc
    10981 gatattccag accaacgact ttgctgccgt cgcctttcac ttccgtggtt tgcgcattca
    11041 gaataatgtc gacgtttttc aggctgcgca gtttgtcctg cagcacctgg tcggctttca
    11101 tttctggcgc aaattccagc agagttacgt gctcaacgat acccgccagg tcgattgccg
    11161 cttccacgcc ggagttaccg ccgccgataa ccgctacgcg tttgccttta aacagcgggc
    11221 cgtcgcagtg cgggcagtag gtcacgcctt tggtgcgata ctgatcttcg cccggaacgt
    11281 tcatgttgcg ccattttgca ccggtcgcca cgataatgct gcgtgctttc agtaccgcgc
    11341 cagaagctgt ttcaatctga tgcagaccac cttcaactgc tgctgggatc agtttgctgg
    11401 cgctctggct gtcgatcaca tcaacgtcgt attcatcaac gtgaactttc agtgcgcctg
    11461 ccagcttctg cccttcagtc ttcggtacag aaatgtagtt ttcgatatca acggtatcga
    11521 ggatctgacc accaaaacgt tcgcccatca gaccggtacg gatgccttta cgtgcggagt
    11581 aaattgctgc cgctgcaccc gccgggccgg aaccgacgat taatacgtca taagcatcac
    11641 gcttgttcag ctcttctgcc gcacgttttt ccgcgccagt atcaattttg gcaacgattt
    11701 cagtcaacgt catgcggccc tgaccaaact ctttcccgtt tacgaacact gccggaacgc
    11761 ccatcacgtt gcgatcggtg atttcgttct ggaaggtgcc gccgtcaatt gcagtgtgct
    11821 tgatgcgcgg gttcagtacg ctcatcaggt tcagcgcctg caccacgtcc gggcagttgt
    11881 ggcaagagag cgagtaatag gtttcgaatt caaaatcacc gtcaatatgg cgaatctgct
    11941 ccagcagaga ctgcgcttct ttcgacggat gaccaccggt ccacagcaac gccagtacca
    12001 gcgaggtgaa ctcgtggccc agcggggagc ctgcaaaacg tggcccctgg ttggaacctg
    12061 ggttggtgat caggaaagac ggcttacgca ccggcaagct gttatcttct ttaaaggtga
    12121 ctttgtctga cagttctgcg atttcagcca acagttcctt gatttctgcc gatttagcgc
    12181 tgtcatccag cgtggcaatt aactcaacag gcttggtcaa tttctcaagg taagccttga
    12241 gttgagtttt catatttgtg tcgagcatgt ttatctcctg ggctttaaac atcatcatgc
    12301 aagcggcctc tgcggctacc tggatgcagc ttgcatcatg gtgcggggaa gaaaatcggg
    12361 tgccgcagca cccgaagaat taggtgaatt tccgaagtat ttcgcgtcag agcaaggcgg
    12421 ctagcctgtg aatccccggg agcttacaca agtaagtgac cggggtgagc gggcgacgcc
    12481 aacgccgcta tggcgtgaaa gacgaaggaa atttagattt taccaaccag gtccagagac
    12541 ggagccagag ttgcttcacc ttctttccat ttagccgggc aaacttcacc tgggtgagaa
    12601 gctacgtact gtgctgcttt gattttacgc agcaggtcag acgcgtcacg gccaatgcct
    12661 tcagcggtaa cttcgattgc ctggatgata ccctgcgggt caacaacgaa ggtcgcacgg
    12721 tcagccagac cttcatcttc acgcatgttg tcgaagttac gggtcagggc gccagtcggg
    12781 tcgccgatca tcgcatattt gattttagcg atggtttcag agctgctgtg ccatgctttg
    12841 tgggtgaagt gagtatcggt agatactgcg tatacgtcta cgcccagttt ctgcagttct
    12901 tcgtagtggt cagcaacgtc acccagttcg gtcgggcata cgaaagtaaa gtcagccggg
    12961 tagaagaaga agacgctcca gcggccttcg gtatcttttt cggtgatttc gatgaattcg
    13021 ccgtttttga atgcctggtt tttaaaaggt ttaattttgg tgttaatcaa ggacatctat
    13081 acttcctccg tgttttcgat gagatgtaag gtaaccaatt tttgccgatt cggctaatgc
    13141 gtttccatta tcaaatcgat aagttttacc ttacaacctt cgtaagacaa ctttgtgaac
    13201 tttgcaccgc cagcggcgat aaaaagtaaa atggccccct ttccaggtga ccgtgtaatc
    13261 tgaactaccc tttggtatct tcagtgcctg cctgcaggct gaggtgttgc ccttacaaat
    13321 gcaacaacga catggattac aacaccctca taaacaaagg gcaatcacct gatctaagct
    13381 cttacctatg acagtgatag gttatgcctt ttactcgact tttgcactga ctgaaaagga
    13441 caaattaatg ttaaaaaaga tacttttact ggctctgctt cctgcaatcg ccttcgcaga
    13501 ggaacttcct gctccagtaa aagcgattga aaaacagggc attacaatca tcaaaacatt
    13561 cgatgccccc ggaggaatga aaggttatct cggaaagtat caggatatgg gcgtcaccat
    13621 ctacctgact ccagatggta agcacgctat ctctggttac atgtacaacg agaaaggtga
    13681 aaacctgagt aacacactta tcgaaaaaga aatttacgca ccagccggac gcgaaatgtg
    13741 gcaacggatg gaacaatccc actggctcct cgacggtaaa aaagatgcgc cggtcattgt
    13801 ctacgtcttc gccgatccgt tctgcccata ttgtaaacag ttctggcagc aggcgcgccc
    13861 gtgggtagat tctggcaaag tgcaattaag aacattgttg gttggggtta tcaagccaga
    13921 aagcccggcg acagcagcgg caattcttgc ctccaaagat cccgcaaaaa cctggcaaca
    13981 atatgaagcc tctggtggca agcttaagct aaacgtgcct gcaaacgtaa gtacagagca
    14041 aatgaaagtg ttaagtgaca atgagaaact gatggacgat ctgggggcaa atgtcacgcc
    14101 ggctatctat tacatgagta aggaaaatac gctacaacag gccgtggggt tgcccgatca
    14161 gaaaacgctt aatatcatta tggggaataa ataaaaggta attcatggat ggttgaacta
    14221 tatccctgct tttcaaccat cctaaaaaag catgaattgc tcgctctgcc ctaagtggct
    14281 gtgctatttt agaaaaactt aatcctgagt taagcaaaaa aacataatca ataaaaatat
    14341 atacgc
//
LOCUS       DAPHBJ010000107        14305 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.108, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000107 DAPHBJ010000000
VERSION     DAPHBJ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.108"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(1..155)
                     /locus_tag="RHZ76_002875"
                     /pseudo
     CDS             complement(1..155)
                     /locus_tag="RHZ76_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(235..1845)
                     /locus_tag="RHZ76_002876"
                     /pseudo
     CDS             complement(235..1845)
                     /locus_tag="RHZ76_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612334.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2140..2550)
                     /locus_tag="RHZ76_002877"
     CDS             complement(2140..2550)
                     /locus_tag="RHZ76_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HDV2813484.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLKR
                     GNDGQPPWFEHDQMTVAAKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSREGGLQEGNWKDRN"
     gene            2736..3614
                     /locus_tag="RHZ76_002878"
                     /pseudo
     CDS             2736..3614
                     /locus_tag="RHZ76_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3615..3785
                     /locus_tag="RHZ76_002879"
                     /pseudo
     CDS             3615..3785
                     /locus_tag="RHZ76_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
     gene            3800..4276
                     /locus_tag="RHZ76_002880"
     CDS             3800..4276
                     /locus_tag="RHZ76_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HDV2813485.1"
                     /translation="MQQISFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            4345..4566
                     /locus_tag="RHZ76_002881"
     CDS             4345..4566
                     /locus_tag="RHZ76_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDV2813486.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            4729..5097
                     /locus_tag="RHZ76_002882"
     CDS             4729..5097
                     /locus_tag="RHZ76_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDV2813487.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPETKK"
     gene            5187..5564
                     /locus_tag="RHZ76_002883"
     CDS             5187..5564
                     /locus_tag="RHZ76_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDV2813488.1"
                     /translation="MKTLPDTHVREVSCCPSLVTIWQTLLTRLLDQHYSLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGFSACTHSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEAKR"
     gene            5561..5764
                     /locus_tag="RHZ76_002884"
     CDS             5561..5764
                     /locus_tag="RHZ76_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813489.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLAELINKVAENGDS
                     LRVVDERDSTETPATYASPHQLL"
     gene            5777..5890
                     /locus_tag="RHZ76_002885"
     CDS             5777..5890
                     /locus_tag="RHZ76_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HDV2813490.1"
                     /translation="MQGIVPLTTETALLSCIKQAREDVLTLRHLQLLHQNR"
     gene            6049..7077
                     /locus_tag="RHZ76_002886"
                     /pseudo
     CDS             6049..7077
                     /locus_tag="RHZ76_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632466.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            7531..7713
                     /locus_tag="RHZ76_002887"
     CDS             7531..7713
                     /locus_tag="RHZ76_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HDV2813491.1"
                     /translation="MNHPVIGVVTKADLASMEHISLVKCWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEDVCR"
     gene            complement(7814..8143)
                     /locus_tag="RHZ76_002888"
     CDS             complement(7814..8143)
                     /locus_tag="RHZ76_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HDV2813492.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEEK"
     gene            complement(8315..9373)
                     /locus_tag="RHZ76_002889"
     CDS             complement(8315..9373)
                     /locus_tag="RHZ76_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDV2813493.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCT
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(9572..10045)
                     /gene="sbmC"
                     /locus_tag="RHZ76_002890"
     CDS             complement(9572..10045)
                     /gene="sbmC"
                     /locus_tag="RHZ76_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HDV2813494.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVFYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(10164..11330)
                     /gene="dacD"
                     /locus_tag="RHZ76_002891"
     CDS             complement(10164..11330)
                     /gene="dacD"
                     /locus_tag="RHZ76_002891"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HDV2813495.1"
                     /translation="MKRRLIIAASLFIFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            11539..12966
                     /gene="sbcB"
                     /locus_tag="RHZ76_002892"
     CDS             11539..12966
                     /gene="sbcB"
                     /locus_tag="RHZ76_002892"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HDV2813496.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFVEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(13009..13236)
                     /locus_tag="RHZ76_002893"
     CDS             complement(13009..13236)
                     /locus_tag="RHZ76_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDV2813497.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(13250..14299)
                     /gene="yeeE"
                     /locus_tag="RHZ76_002894"
     CDS             complement(13250..14299)
                     /gene="yeeE"
                     /locus_tag="RHZ76_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001647169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HDV2813498.1"
                     /translation="MILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYALLI
                     AISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRAGEG
                     LIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIADTFNLSVWPLVAVLLVI
                     TLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEAT
                     GRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASSEFRVRAADA
                     QTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASWLVY
                     VRPQRKARLATAAAN"
BASE COUNT         3704 a         3748 c         3387 g         3466 t
ORIGIN      
        1 cttgagcgta tccggactaa acccttttca acacgcagct cgtccggcaa ccactgatgg
       61 tggaaacgag gcgcattggt cgcttcggca acattcattc cacaattgat gctgttcacc
      121 accatttgca gcacggtagt gatgattcag ctaccggtaa caaagtgagt acataaaggc
      181 catactgtca cgaacagtgc aaaagtggct cttccgcaac ctaggtgaag attttcatgc
      241 cagtggactc attaccctcc gcctaagtag ttcaaatccg gctcgaccat acatctggcg
      301 tatcagcacc ttcaggcgat ttacatgacc ttcgacaaca ccattactcc agcgactgct
      361 gattgcctca catatagccg cagcatcagc ttccatcccc gcagctacgc accgaagttc
      421 tgctgagccg ctttcgtgga ctcgagtgaa ccagctgctt agctgtgatt tattttaggt
      481 tttcagaata cggtagaact cgagtaccag ttgctgtgct attttcaact ccggttcttt
      541 ttcacacatc agactaataa atcgggatgc ataattttct tcgcccctga ttattctcca
      601 gggcatcaac cagcggctta cccgggacac tgaaggaagt cttgcggcag tagtaaccgg
      661 tgggttccag cctttacgcc atttggcaac agtatccctg acgatggttt cactgcctgt
      721 cccccccccc tgggccacca tttcccgcca tatccggctg gcattataat taccactttc
      781 tcgttgctct tttaaccact ccctccatgg gtccagaagc cctcgctttg gaggccgggt
      841 tgacatttca gggaatgttc ctgaacgcac ccagcgactg actgtcactc gcgacaggcc
      901 tgtaatacgg gatatttccc tgaaactaca tcccttgtta tgcagggcca taacctctgt
      961 ccaatgctga tgccgttttt tgcgggtttg ctgtttaagg cgttccggac gacggagcga
     1021 tgctacaggc acagatattt ctggctcagg tgatttcttc agtgataact ctctgacaac
     1081 aagacgtatc agaggcatat gtctgtacat cattcgttca ggctcatcac caatattttt
     1141 tagcaggtgc catcgatcgg ccacctgtct ggcctgaggt gcaccttcac gtgctgctgt
     1201 cgcatagact ccactgcgat cacgcgagac aacctgtatt tccggatatt ttctgaacca
     1261 ggtcgccagc gtacgctgat cacggccggg aagcaggacg aggggacggt gagtatcaag
     1321 attgactatt aacataccgc agcagtggcc ccgatgccac gcccactcat caataccgac
     1381 atgaggcgcg cctgactgtt tcgtctccgg ggtattgata accctgcgaa gaagagtatc
     1441 agcactgcaa cggagacccg ctgccgttgc agcccgtttt ccagcctcac ctccagctat
     1501 taatcccagt tgacgttgta aattttgtaa cgcctgtgaa gactgttggt gtgaaccggc
     1561 gaagggagca agcgactcgg caaaaatttt acgtgaacaa gctgggttac ggcagtacca
     1621 gtggcggacg gaaaatacaa gccatagcgt ctgccctgat cagggtagat gctgtatccg
     1681 gcgccgacaa caactatgaa cagagtcgct acgcttaaag cattcagggc attgtgctgt
     1741 tttgcgtttt ccgtggagat ggagggttat accgtcagag ctaataattt gtcgactgca
     1801 tcgccatccg taaggtaact gtagaagtgt cttaagagag tgcattgggt gcccataact
     1861 taaaacagct tagatccttg ttcgccagaa agtttcatcc attttcttcc acccaggttg
     1921 cggaagaacc acttttcatc cgtcgttgac agatgtgaag tatgcgggag aaaaagtgaa
     1981 tatctaactg taataactca gaccggtacg agtactcggg gcgtctacaa tcattcaggg
     2041 caaagtgcta aaatttctct atcccgggat gcccctacca acgcagcact cccggagata
     2101 tcattacagc tctgcaactg atttccgcga ctgaaccgct cagttcctat ccttccagtt
     2161 gccttcctgc agccccccct ccctgctgta taccccagta ccacaacgcg ggcagcactt
     2221 ttccccctca taatcttcgt cacacatcac acaaaaccac tttctcacct tcaccgtata
     2281 atccggaacc gtcatgccct gcagatgcaa acgacgggcc tctttctctg ccgggtcaag
     2341 ccaggtgcat cgcagcagga ttttcgcagc aacagtcatc tggtcatgtt caaaccacgg
     2401 cggttgtccg tcatttcccc gcttaagtgt taacggacaa ccacaggaca cacagcacaa
     2461 aacgtcctgt cggttgtgct tcgcggcttc tttcgccgta acaaaatggc ctttgctgtc
     2521 attcgccata tagcagttca gaattcgcat atctcctcct tgtatcttga caattggtta
     2581 aaataactgt gattagattt ggcaaggtgt atgcagcccg atccctcaag gggttcatag
     2641 cccgtagctc agcgaatggc gcatacactg agccactcat ctgaaacccg aacagttgtc
     2701 agtacgccat aacgcatttg caaggatggg taatcatgag tcagccaggt tcactttgtg
     2761 tggatatcga tgtttcgaaa gcgatactgg atattgccgc cagcagcgct attgaacaat
     2821 tttctgtcgg caatgacagt gatggctttg acgccatcat tgctgagttg agaatgtacg
     2881 ccattgcgct ggttttaatg aaagccactg gaggacttga aaccgccata gtctgtgcgc
     2941 ttcaggcaga aggttttgag cttgccgtgg ttaaccccag acaagctcgt gactttgccc
     3001 gcgccatggg atacctcgcg aaaactgata gtattgatgc cagagtgctt tcccagatgg
     3061 ctgaggttct caaccggcat ccggagcggg aacgctttat cagagcactg ccggatactg
     3121 aatgtcaggt tctcactgca atggtagttc gtaggcggca gttgattacg atgctggttg
     3181 ccgagagaaa ccggttgcac cctgctcatc catagaacag gaaaagcatt aatatcatca
     3241 tcaaagcact ggaagacgag cttgcgcgac tagatgctga catgaacagc catatccata
     3301 gccacttcaa agagcttgct aagcgactga gcagtattaa aggtgttggc accatgacgg
     3361 ctgctgcgct gctggcggaa gtacccgaac agggtaacct ctccaggcgt gaaatcagtg
     3421 cgcttgtcgg tatggttcca gtaaaccggg actcgggcac gatgagaggg cgacgaacca
     3481 tctttggcgg cagggctggt gtgcgaacag ccctgtacat ggcagcgctt gtcgcaaccc
     3541 gctttaaccc ggtaataaag gtgttttaca tgcgactgct tgccgcagga aaacccaaaa
     3601 aagtggcgct ggttaaccgc gctgaaatga gcccggaagc cgccggcatt gctgcctgtc
     3661 tgatgacgta cagtcatcat gcctgtcgta cggagtgtta tgccatgacg gtccattatt
     3721 accggctgcg ggattacgcc ctgcagcatc cggaatgcag cgccattatg cgcatcattg
     3781 actgaaaggg gccggaacaa tgcaacagat ttcctttctg cccggagaga tgacgcccgg
     3841 cgagcgcagt ctcattctgc gggccctgaa aactctggac cgccatcttc atgaacccgg
     3901 cgtggccttc acctccaccc gtgcggcacg ggaatggctg attctgaaca tggcgggact
     3961 ggagcgtgaa gaattccggg tgctgtatct gaacaaccag aatcagctga ttgccggtga
     4021 aaccctcttc accggcacca tcaaccgcac agaagtccat ccccgggaag tgattaaacg
     4081 cgccctgtat cacaatgccg cagccgtggt gctggcacac aatcacccgt ccggtgaagt
     4141 cactcccagt aaggcagacc gccttatcac ggaacgtctg gtacaggcac tgggcctggt
     4201 ggatatccgg gtgccggacc atctgatagt cggtggcagc caggttttct cctttgcgga
     4261 acacggtctg ctttaacccg tcaccgccac aatcacatca cacctgtttt cacttttatc
     4321 ttccgtcttc agaggtatcc tattatgaaa attatcaccc gtggtgaagc catgcgtatt
     4381 caccaacaac atccgacatc ccgtcttttt ccgttctgta ccggtaaata ccgctggcac
     4441 ggcagtgctg aagcgtatac cggtcgtgaa gtgcaggata ttcccggtgt gctggccgtg
     4501 tttgctgaac gccgtaagga cagttttggc ccgtatgtcc ggctgatgag cgtcactctg
     4561 aactaatgtt ccgtcacccg gaaaacgagt acgactggca gagctcagac gccgctgtgg
     4621 atgaggtcct gctcatcaac gaatacacct ttaccgaagc cggtctgcgc gccggctgac
     4681 ccgtcatcgt taaactatca ttcaccgatt ttctgtgagg attttatcgt gtcagacaca
     4741 ctttccggga caacgcatcc cgatgacaat gacgaccatc cctggtgggg gctaccctgc
     4801 acagtgacgc cctgtttcgg cgcccgtctg gtgcaggagg gtaaccggtt gcattacctt
     4861 gccgaccgtg ccggtatcag aggccggttc agagatgcgg atgcgtaccc tctggaccag
     4921 gcctttccgc tgctgatgaa acaactgaaa ttaatgctca ccagtggtga actgaatccc
     4981 cgccatcagc ataccgtgac actgtatgcg aaggggctga cctgtgaagc cgacaccctc
     5041 ggctcctgtg gttacgttta tctggctgtt tatccgacac ctgaaacgaa aaagtaaacc
     5101 accagaataa ccttctgctc cggcctggtg ttttcaccac gccactgtcc cattttttat
     5161 ctctgcatat caggaaaatc ttcagtatga aaacattacc cgacacgcat gtacgggagg
     5221 tatcgtgctg cccgtctctc gtcaccatct ggcagacact gctcacccga ctgctggacc
     5281 agcattacag ccttacgctg aatgacacac cgttcgctga tgaacgtgtg attgaacagc
     5341 atattgaggc aggcatttca ctgtgtgacg cggtgaactt tctcgttgaa aaatacgcgc
     5401 tggtacgtac cgaccagtcg ggattcagcg cctgtaccca ctctcagtta ataaacagca
     5461 tcgatatcct ccgggctcgc cgggcaacag gcctgatgac ccgcgacaat tacagaacgg
     5521 taaataacat taccctgggg aagcatccgg aggcgaaacg atgaaactgg ccctgacgct
     5581 ggaagccgac agcgttaacg tgcaggcgct gaacatgggg cgcattgtcg ttgacgtcga
     5641 tggtgttaat ctcgctgaac tgattaacaa ggtcgctgaa aacggtgatt cactccgcgt
     5701 ggtggatgaa cgtgactcta ccgaaacacc ggcaacttat gccagccctc accagttgct
     5761 gtaagacaat gcagtgatac agggtatagt gccattaacc acagaaacag cactgttgtc
     5821 ctgtattaag caggcaagag aagatgtcct taccctgcgc catctgcagc ttctgcacca
     5881 gaaccggtga atctcactca acaggcttca tttcccctga cgccagcctg aatacagctg
     5941 gcgttttcat ttataaacag aaaggaaaac cgataataat ggaatggacg cccctaccat
     6001 gaagcaacgg taggatatta ctgataaacc ttccagtgga ggtgcgttat ggccgtttta
     6061 acgattagca ttgacctgac aaaaaatgaa ttccaaatcc acggtctggg taggaacaga
     6121 aaaatctaag ctcagaaaac ggattaaacg ttccctgatg cccgtattct tcgcgaatat
     6181 gtcttcctgt cggattggga tggaggtctg cgccggagcg cgtcattagt gagaatgctg
     6241 tctacttagg ggcataatgt cagactaatg cccgcgattt gtaaagccat atgtgaaaac
     6301 aaacaagaat gatatggctg ctgccgaggc catctgtgaa gccgttaccc gacctaatat
     6361 gcggtttgtc agcatcaaga cgcctgagca gtagtcatta ctgccttttc atcgtgccca
     6421 aacgggattt gttaaatcaa ggactgcgca gataaaccag atcagagaat tactggctga
     6481 atttggcatc gttctcccgc ccgacattgt agcattcagt cgtcatgtcc ctgccctgct
     6541 tgaagacgcg gaaaactgtt taacaatgcc gttcagacag ctgtcgtcct atctctaatg
     6601 aaaatgtgaa acaactgagc gtacacattg aaaccataga agccgtgagc aacgaacacg
     6661 tcgggaacga cgaactgtgt aggaaaattg ccaccatttc ggggattgct attcttacca
     6721 ccactgcaat tgtcagcacc gggtagtatc ggcttcaaaa accggcgtca gcttgctgca
     6781 tggcttggac tggttccccg acagtgcttc agcgtaggta aaaacacgct ggggtctatc
     6841 agtaaacggg acgatgtcta tatcagaacg ctactgttcc atggtgcgaa gacagtgtca
     6901 aacgccagaa agggcagaca ggtccaggaa agttggctgg gcagaccagt ctcgcggcgg
     6961 aataaaaatg ttgccaccgt agcgctgaac aacaaaaatg ccagagtgct ctgggtattg
     7021 ctaaccaacg ataaagagat ttcaccagaa gaagtgatgg tagctgcata tatgtaaatg
     7081 aaactacttc tgtctgttga aaaataaaac caacctactg attgctaagg ataaacaagt
     7141 attaaaggcc agactgataa gaccgcgact aagtgaacct gtatctgaca tggcatagct
     7201 tcgctaatgt gtcatcatga ttaggactaa atctgtgaat tcctttagga agcacggaca
     7261 caggccaatc tgagtccata tgtatgattg gaatcttaca cagatgactt tttgacacgg
     7321 ggacttggca aacaggggcg accatgtatg tcagatatga caatctcact tccgaaagaa
     7381 gtcaacgggc acgttatctg ttcaaccaga atccgacaca ttctggctat acgaaagagc
     7441 atacttatgc aaatctatat gcttttccgg caacaagcag gaatctcagc gctgaggcag
     7501 gaattagagg taaaaacgcc ctaccgggcc atgaatcacc cggtcattgg agtagtaacc
     7561 aaagcagatt tagccagcat ggaacacata tctttggtga aatgttggtt acgggaagct
     7621 ggagcgcaca acgtgttagt aaccagtgcg gttaataaca atggagttac agaacttttt
     7681 gccttgctgc atacagaaga tgtctgtcgc taatttcgat aggttctcag atgagaatga
     7741 tgacggtgat acacagataa gaatcaatga gcaatgaggg ttgccgggca accctcattg
     7801 aataaaacag gaattacttc tcttcgccgt ttttcatttc gcccatagct ttcagctttt
     7861 tggagatatc gcggcgttct ttggagagct cggcattttt gatgatgtaa tcgtcaacac
     7921 gatcttcata gtcacctttc atgctggcga tgatgccctg gattgcttca acgctcatcc
     7981 ccggcttgat gtaatcgctc aggttgtcca gcagcaggac gcgcttctgg ttgtcacgga
     8041 tctttttctc aacgtcctga atttcacgtt gcagtttgtt cttacgacgg aacagacgaa
     8101 caaattccag tacgtcctgg aatgaaggct tggtagtttc catttgtata cccctgataa
     8161 tgtgagagtc ggattcgttt aagcaaccgc tattcgttag ggccaacatt actcatagct
     8221 gccgcagatg acaatgcttt tatccctttt cactatcata ccctttatcc ttgctgaatc
     8281 gaagcagcag caagatgatt ctgaagttca ggaattattt gcgcaaggcc cggcaaatca
     8341 gattcgatag caactcaagc tgatgtgccg tttccatgtt cagccacaca taaccataga
     8401 ttggtgtttc cactaccttc aggtcatggt ggttattgag caactgacgc agctcttcca
     8461 cagcatcgtt caatttttcc gtattggcaa aaaccggctg cggattacct tcgtacagcg
     8521 catgaacaag gctcagcagt atttgctgca tcatgtgctg ggtatcacga agtttttgcg
     8581 cgttcaataa cacgaaatgg ctggggcgcg tggcccagta tgcattgatt tgcaactcca
     8641 gcatacaaac cagattgcgg ttaatggtct ggataccttc atatatcgat tttggaatac
     8701 gagtttcttt gctggcgggc gcaatcagcc cacgcatttt cacggcatcg gtcaggagtt
     8761 tttgtagatg gctttccaga cgtgggcgtt cgagtaagtt cggtgagaat gcagattgat
     8821 aaacccgatt atactcggtc agacttttcg ccagttgaat gcgccagtgg atgaacgccc
     8881 gttgtggcca gataccggta aacaacattg ccagtaaaga gccgaggatc acatcgccgc
     8941 ttcgccataa cgccgtatca atttcacctg tcggggaacc cacaacaatt gccagcgtca
     9001 ccccaatcaa taatccttga tacggtttct tgcccagcgc cagccaaccg caaagaaaca
     9061 tagccgccgt gcaccagact aacatcagcg gtaacgaaat taactccagt tgcagagcga
     9121 taagacctaa aatcgaaccc aacaccgtac cgccaatacg ttcgaaggcg cgagggacaa
     9181 cgttacccca gaacgagatt ggccccataa tcaccaccat ggtgaccagc ggccaggtgc
     9241 tttccgggat agtaaacagg cggataatga gaaaagtgag caggaatgcc agcgcgaccc
     9301 gagtaccatg cacaatgcgg taatggcggt atacccggat ttcaaacggg cttaatgact
     9361 tatcggcacg cacgcagaaa ctccgtaatg aagattaaaa gcgctcataa ctcatctgtc
     9421 ggccagaagg cccgcctctg aattatccga cggacaaacc gggaacagtg taatttcccg
     9481 gcaggagcga aaaaattgaa aggcgcatct tactcttttc gctttcaaaa aaaagagtgg
     9541 tcatcgcgtt aacacaccgc cctgagatga attagtgatg ttttggctgc accgcaacat
     9601 acatttcgat atcccagtac ccatcttccg cgccattgtt caaataaacc tcgaagcagg
     9661 gctttggtaa catttcataa gcactgtcct gcaagaggct attaaagaac tgataccagg
     9721 gtttagcaaa atcatcaccg actacacgag ccaccgcaac cgcatactga ccacctgaaa
     9781 tttctgtcag aatgacgccc tcactgtttt cggggagcgt aaagttattc ggcaccgtca
     9841 cgacggtgtc gcagcgtaat ttttcggcgg gtgtttcatc tggattgtcg taaaagacag
     9901 caacccactc cttcggcaca atatttttgc tatctaccca catcatcaac tgctcaaagc
     9961 ctttctttac cgtctgttcc cacgggccaa cgagatggaa acctgcaacg gtacgtttat
    10021 cttcctgctt aatctcgtag ttcatgacgc ccccattaac actgttgtta tatacagtat
    10081 aattgcgata ttaaaaccac aaggaatgag tgttgattat gcgagcagac tcgcactcct
    10141 gccagtctgc tgcaaaagaa aggtcaggcc ttatggtgga aataatcact caggcgagaa
    10201 aacatgctgc cttccccgac agattccagg gtaaccagcg gccagtgcgc cacctgttta
    10261 tcacggtcgt aaagttcaat ttcccctacc cgctgatggg cgctaattgg cgcggtgagc
    10321 tctttaccat caagggtata tttggctttg atatgtggaa tttcggcttt cggtagcacc
    10381 atccagaact cttgttccgt tcccaggtcg atattttctt tatcgccata ccagatgcgt
    10441 tccgtaccga cctttttccc acggtgcaaa atttgcaccg tagtaaagtt ttgttgcccc
    10501 caacgcagta attttcttgc ctcttcctca cgaccttttg cactgtcagc ccccattacc
    10561 actgcaatga gacgacgctg cccatcgaca gccgaagcaa tgagattaaa cccggcacca
    10621 gaagtatgcc ccgttttcag gccgtcaaca ttcatggttt tatcccacaa taacccgtta
    10681 cggttttgct gggtgatacc gttccaggtg agacttttct cactgtacat atgataaaac
    10741 tcgggctcgc cgtggatgat agcgcgagaa agcacagcta aatcataagc cgagctatgc
    10801 tgacctggtg catccagacc atgcactgtt tcaaaatgcg tatccttgag atgcagcttc
    10861 tcggcatagt tgttcatcat ttcaacaaac tgccgttgcc caccggcaat atagtcagcc
    10921 agtgcaacac aagcgtcatt tccggaatcc acaattaaac cacggcttaa atcacgtacc
    10981 gatacgcgat cgccctcttt caaaaacatc agtgaagaac cgacaaacac tggattatct
    11041 ttcgcccacg catcgcgccc cacggtgaca atatcgtctg gcgtaatgcg atgactatcg
    11101 atagcgcgat ccacgacata gcccgtcatc agctttgtca ggctggcggg attgcgctgt
    11161 tgatgctcat tacccgcggt gaggatctga ccggtggtgt aatccatcag tacccaggag
    11221 ccggcatgaa tctctggagg ctgaggtgaa aaaggaatgt tttccgccgc aaaaccagac
    11281 gataagttaa aaatgaacaa agaagcagca ataataagac ggcgtttcaa cagcaaaccc
    11341 tcaggagttt caaatagctg ttctttttac ggaaatactt atgaactggc tggaataaag
    11401 tgcaagaaaa tgtgactacc ctctcatttt tatctgacat gatctgttgt cactcgctgt
    11461 caaattgtcg cgctaaagct gattagcacg gtgatatttg atactctggc agacagcaga
    11521 aataacggat ttaacctaat gatgaatgac ggtaagcaac aatctacctt tttgtttcac
    11581 gattacgaaa cctttggcac gcaccccgcg ttagatcgcc ctgcacagtt cgcagccatt
    11641 cgcaccgatg acgaattcaa tgtcatcggc gaacccgaag tcttttactg caagcccgcg
    11701 gatgactatt taccccagcc tggagcagta ttaattaccg gtattacccc gcaggaggca
    11761 cgagcgaaag gagaaaacga agccgcgttt gccgcccgta ttcactcgct ttttaccgta
    11821 ccgaagacct gtattctggg ctacaacaat gtgcgtttcg acgacgaagt cacacgcaac
    11881 gttttttatc gtaatttcta cgatccttac gcctggagct ggcagcatga taactcgcgc
    11941 tgggatttac tggatgttat gcgtgcctgt tatgccctgc gcccggaagg aataaattgg
    12001 cctgaaaatg atgacggtct accgagcttt cgccttgagc atttaaccaa agcgaatggt
    12061 attgaacata gcaacgccca cgatgcgatg gctgatgtgt acgccactat tgcgatggca
    12121 aaactggtaa aaacgcgtca gccacgcctg tttgattatc tctttaccca tcgtaataaa
    12181 cacaaactaa tggcgttgat tgatgttcct cagatgaaac ccctggttca cgtttccgga
    12241 atgtttgggg catggcgcgg caatacaagc tgggtggcac cgctggcgtg gcatcctgaa
    12301 aatcgcaatg ccgtaattat ggtggatttg gcaggagaca tttcgccatt actggagctg
    12361 gatagcgaca cattgcgcga gcgtttatat accgcaaaag ccgatcttgg cgataacgcc
    12421 gccgttccgg ttaagctggt gcatatcaat aaatgtccgg tactggccca ggcgaatacg
    12481 ctacgcccgg aagatgccga ccgactggga attaatcgtc agcattgcct cgataaccta
    12541 aaaattctgc gtgaaaatcc gcaagtgcgt gaaaaagtgg tagcgatatt cgtggaagcc
    12601 gaaccgttta caccttcaga taatgtggat gcacagcttt ataacggctt tttcagtgac
    12661 gccgatcgtg cagcaatgaa aattgtgctg gaaaccgagc cgcgtaattt accggcactg
    12721 gatatcactt ttgttgataa acgaattgaa aagctgttgt tcaattatcg ggcgcgcaac
    12781 ttcccgggga cgctggatta tgccgagcag caacgctggc tggagcaccg ccgccaggtc
    12841 ttcacgccag agtttttaca aggctatgca gaagagattc agatgctggc gcagcagtat
    12901 gccgacgata aagaaaaagt ggcgctgtta aaagcgcttt ggcagtacgc ggaagagatt
    12961 gtctaatgtg aagcctccgc cgctggtacg gcggaggaca attttgagtc aggctttttg
    13021 aacggtgatg ctccacgctg catcgccaat ctgctgataa tcggtgatgg catgtccttc
    13081 ttctgccgcc cactgtggga ttgcttcagt tgcctgggtg caatcaaact caattaccaa
    13141 ctcatcgccg ctggccattt ctgccagtgc tgcttttgct tctatgagtg gaaacggaca
    13201 gacctgcgtc accacatcca gctttttaat cgccatcttt acaccttact taatttgccg
    13261 cagcagttgc cagtcgtgcc ttacgctgcg gtcgaacata cacaagccag gacgcagtcc
    13321 agactccgag aatcataaat acaaggccaa tccagccctg ccaggtcatc attgcagtca
    13381 tgaccagtcc attaccgata gagcaaccac ctgcaatgct ggcaccgaag cccatcagta
    13441 caccacctaa cccgctacgt aatgttgttt gtgcatcagc agcgcgaacg cggaactcac
    13501 tgctcgcttt ggctgcaata aatgacccca cgaagatccc taacaccagg aaaacgcccc
    13561 agttaatgta tttcacgtca cccgcgacca gaaattgcag aatattggcc gttggggaag
    13621 tgattcctaa cccaaacatg cgtccggttg cttcactcag gggccaggct aaaagcgcaa
    13681 tcaaaccgat gagtacagca gtgacaaagg gatgccagcg tttttcaaac agaatatgag
    13741 cgatcccggt tcggcgcggc ggtaaggtcg cgactttgag ttttggcttc ttcaactctt
    13801 tcatcaccac ccagagcgtt atcaccagca aaacggcaac caacggccac acagacaaat
    13861 taaaagtatc agcaatagag ttatgttcag tagtgtagtg ctgcaaggtt tgatttaaac
    13921 cactggcgtg tggagaacgc atcaccgcac tcatcaccat ataagtgaaa agcgcgatcc
    13981 aactgccgat caatccctca cccgcgcgat accaggtccc ggtggcacat ccacccgcca
    14041 gaacaattcc cagcccgaaa agatacccac ctataacagt accaagccac gggaacgccc
    14101 cggcttcgta agtcagtagg cccgcctgaa ttaacgcaaa aacccccacg ctttgtaccg
    14161 aaattgcaat cagcaaggcg taaaacatgc gattattttt cgcgatatac atatcgcgaa
    14221 aaccacctgt caggcaaaaa cgcccacgct gcatgacaaa tcccagcaga gcaccacaaa
    14281 ttagcccgct taatatcatt gaaaa
//
LOCUS       DAPHBJ010000108        14192 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.109, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000108 DAPHBJ010000000
VERSION     DAPHBJ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.109"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..119)
                     /gene="yeaR"
                     /locus_tag="RHZ76_002895"
     CDS             complement(<1..119)
                     /gene="yeaR"
                     /locus_tag="RHZ76_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HDV2813499.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGV"
     gene            complement(292..930)
                     /gene="leuE"
                     /locus_tag="RHZ76_002896"
     CDS             complement(292..930)
                     /gene="leuE"
                     /locus_tag="RHZ76_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HDV2813500.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NSEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILAATL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(1057..1980)
                     /gene="dmlR"
                     /locus_tag="RHZ76_002897"
     CDS             complement(1057..1980)
                     /gene="dmlR"
                     /locus_tag="RHZ76_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HDV2813501.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGPHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            2083..3168
                     /gene="dmlA"
                     /locus_tag="RHZ76_002898"
     CDS             2083..3168
                     /gene="dmlA"
                     /locus_tag="RHZ76_002898"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HDV2813502.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            3419..5029
                     /gene="yeaV"
                     /locus_tag="RHZ76_002899"
     CDS             3419..5029
                     /gene="yeaV"
                     /locus_tag="RHZ76_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HDV2813503.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVTWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            5061..6185
                     /gene="yeaW"
                     /locus_tag="RHZ76_002900"
     CDS             5061..6185
                     /gene="yeaW"
                     /locus_tag="RHZ76_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HDV2813504.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            6241..7206
                     /gene="yeaX"
                     /locus_tag="RHZ76_002901"
     CDS             6241..7206
                     /gene="yeaX"
                     /locus_tag="RHZ76_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HDV2813505.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHVI
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGNRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(7260..8375)
                     /gene="rnd"
                     /locus_tag="RHZ76_002902"
     CDS             complement(7260..8375)
                     /gene="rnd"
                     /locus_tag="RHZ76_002902"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HDV2813506.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDMTNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(8457..10142)
                     /gene="fadD"
                     /locus_tag="RHZ76_002903"
     CDS             complement(8457..10142)
                     /gene="fadD"
                     /locus_tag="RHZ76_002903"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HDV2813507.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(10347..10928)
                     /gene="yeaY"
                     /locus_tag="RHZ76_002904"
     CDS             complement(10347..10928)
                     /gene="yeaY"
                     /locus_tag="RHZ76_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HDV2813508.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(10968..11663)
                     /gene="tsaB"
                     /locus_tag="RHZ76_002905"
     CDS             complement(10968..11663)
                     /gene="tsaB"
                     /locus_tag="RHZ76_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDV2813509.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPEIVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(11721..13631)
                     /locus_tag="RHZ76_002906"
     CDS             complement(11721..13631)
                     /locus_tag="RHZ76_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDV2813510.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13763..14107
                     /locus_tag="RHZ76_002907"
     CDS             13763..14107
                     /locus_tag="RHZ76_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDV2813511.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         3456 a         3752 c         3519 g         3465 t
ORIGIN      
        1 taaacccccg ggcgcgttcc tttatcaaga tgacgttcga atattccggc aggtgcagtt
       61 tgtttattcc agaaaggcgt tgagcgcgta tgaatataat tctgtgggat ttgaagcatc
      121 cttttccctc cttcggtgaa tgcgctgaaa acggtttatt ccagccgttt cagggtacgc
      181 ctgataattt gcattttaaa taccatttat tggttacttt ttagcaccat atcagcgaag
      241 aatcagggag gattatagat gggaagcccg cgacaacgcg ggctgaaagc atcaggattg
      301 cagcgtcgcc agtcgggcag cgaaacccac gaacatcaaa ccaatcagtg agttgccaac
      361 tttagccagt ttctttttgg tacgtatgta ctgcgtgaca aaagcaccag atataatcag
      421 gaagctcaaa tagcagaaac tcaccagttc cagcgtcgcc gccagaataa agaatgaaat
      481 tcccgtatgt ggggcattaa catcgataaa ctgtacgaaa aacgacacat agaacaaaat
      541 ggctttcgga ttagtcaggc tcaaaattaa cgcgcgttta aaaatagcac catattgggg
      601 ctcatcggat ttggcctcgc tatttttacc cttcagggtc gcgtaaagaa ttttactccc
      661 cagatagagc aaataaaacg caccaagata acgtacaatg ttgaataata tcggggtggt
      721 cttaattaat gtcgccactc cagcccatgc cagaaacatc aataccgcat cgccaataaa
      781 tacaccgcag gccgcaagat aaccgccttt cataccgcta ctgacgctat ttttgagtac
      841 aaacagggta tttggccctg gcaccaacac aataaaaatg gccccaacca gataggtcca
      901 gtaattcaga accccgtatt cagcgaacac attaacctct ttaattatct ttcgatcatg
      961 cgcgattaaa ggtgaatatg ctaaccaatc tgtagcggct tagaaaggag aaaatcaggt
     1021 tttaacctga tatcaacccg ataattgaat cattaatcag gcatgctcca gtgaaaaatt
     1081 cgggtagtgc tcggcaaaat actggcgtaa aaattctacc gttatccgca ctttcgctga
     1141 cgtcgccagc cttgaaacat aaacggccca gacgttcgct ggctggtaat attccggtag
     1201 cacttgcact aaatgaccac tggcaatgtt ttcgctaaca tcccaccagg aacgcagagc
     1261 aatcccctgc ccatcgagac accactgatg gacaatttct ccatgattag acgacagtgg
     1321 gccggtgact ttaatcgcat gggggccttc tttattgcgt aattgccaga caccaaaagg
     1381 atgatcgcgc tctttaatga ccaggcacgg caatgcagaa agatcggtta aatgtttcgg
     1441 cgcgccatgt tgcgcaataa attccggcga cgcgcagaga atacggtaat tggtcgccag
     1501 tttgcgggcg attaaattag gggcgatgtc atcgccaatg cgaatatcga gatcgacacc
     1561 ttcattgacc aaatcgacca gtcggtcttc cacatcaaaa cgtaattcca gttgcggata
     1621 cgccttcgcc agcgctgata acgccggagc caccacctgt cgtccaaaac caaagctgct
     1681 gataatacgc agcatcccct gcggcacctg acgcacgtca gaaagttcgt ccatcatctg
     1741 accgacatcc tgcaaaatcc gctgcgccca ttcataaatc cgctctcctt cttcggtaat
     1801 ggtgacgcgg cgggtggtgc ggtgtaacaa caccacgttt agcgtttgct ccagcaaggc
     1861 gatgcgcttg ctgacgaacg ccggtgaaac gcccagttct tccgccacgg cggcaaaacc
     1921 agcccggcga gccaccagca taaagacgcg caaatcattc agcagcggta aattattcat
     1981 gattcgtgtt ttatgtttca ccagttacgg ggattaattc cttttcagtc aattatagga
     2041 tggtgatgtt gtcaattttg atggtcagga agtgagaacc caatgatgaa aacgatgcgt
     2101 attgctgcga tcccgggaga cgggattggc aaagaagtcc ttcctgaagg gattcgcgtg
     2161 ttacaggctg ccgctgagcg ctggggcttc gccttgagtt ttgagcaaat ggagtgggcg
     2221 agctgcgagt attacagcca tcacggtaaa atgatgccgg acgactggca tgagcaactt
     2281 agccgtttcg acgccatcta ttttggtgcc gtcggctggc cggataccgt tccggaccat
     2341 atttcgttgt ggggttcgct gctgaaattt cgtcgtgaat tcgaccagta cgtcaacctg
     2401 cgcccggttc gtctctttcc tggcgttccc tgcccgctgg cgggaaaaca gcctggcgac
     2461 atcgattttt acgtggtcag ggaaaacacc gaaggcgaat attcctcgct cggcggtaga
     2521 gtgaatgaag gtacagagca tgaagtcgtc attcaggaat cggtatttac gcgtcgtggt
     2581 gtcgatcgca ttttgcgtta tgccttcgaa cttgcgcaaa gccgcccacg taagacgcta
     2641 acttctgcca ctaaatcgaa cggtttagcc atcagcatgc cgtactggga tgagcgagtg
     2701 gaagcaatgg ccgagaatta cccggagatc cgctgggaca agcagcatat tgatattctc
     2761 tgcgcgcgtt ttgtgatgca gccggaacgc ttcgatgtgg tggtggcgtc caatttgttt
     2821 ggcgatatcc tttccgatct tggcccggcc tgcaccggca ccattggcat tgccccatcc
     2881 gccaacctga atccggaacg cactttcccg tcgctcttcg agcctgtcca cggttccgcg
     2941 ccggatatct acgggaaaaa tattgctaac cctatcgcca cgatttgggc cggggcaatg
     3001 atgctcgatt ttctcggcaa tggcgatgag cgtttccagc aagcgcataa cggtattctg
     3061 gcagcaattg aagaagtgat tgctcacggg ccgaaaacac ctgatatgaa aggcaatgcc
     3121 accacgccac aggttgccga cgcgatttgc aaaattattt tgcgttaagg tcaaaccagt
     3181 ttatttgaac cgcgtcactg acgcggtttt tttattcgtt ctttgcagta aataacctgt
     3241 gtcatttcac cttttattgt ttccgtttcg tgttttatgg ctttccgtat tcttaattgt
     3301 ttaatttatg taacatacaa ataatcaatt tgttaaacac aacatacaac tcattaccct
     3361 gcaaataatt acaactggca aaagccagag acgggctttt atgcccagga gatgactcat
     3421 gatgagcaat gttaagaaaa aagatgtgcc gctgataagc atcagcctgg tggccattct
     3481 tttcatcgca gctgcattaa gccttttccc acaacaatcg gccgacgcgg ccaacgcaat
     3541 atacactttt gttactcgta cgttaggttc cgccgtacag gtattggttt tgctggcaat
     3601 gggactggtg atttatttag ccaccagtaa atacggcaat attcgtcttg gcgaaggaaa
     3661 accggaatac agcacgctct cctggctgtt tatgtttatt tgtgccggtt taggttcttc
     3721 tacgctttat tggggggttg ctgaatgggc ctattattat caaacacctg gattaaatat
     3781 cgcaccgcgt tcacaacagg cactcgaatt tagcgttccc tactctttct tccactgggg
     3841 catcagcgcc tgggcaactt atacgctggc ctcattaatc atggcttatc actttcatgt
     3901 gcggaaaaac aaaggtctga gcctttccgg cattattgct gctattaccg gcgttcgccc
     3961 gcaaggccca tggggaaaac tggtcgattt gatgttcctg atcgccactg tcggcgcact
     4021 gaccatttcc cttgttgtta ccgcagcaac ctttacccgt gggctttccg cgctgaccgg
     4081 tttacccgat aacttcaccg tgcaggcatt tgtgatcctg ctttccggcg gcattttttg
     4141 cctaagctcg tggattggta tcaacaacgg tttgcaacgt ctgagcaaaa tggttggctg
     4201 gggcgcgttc ctgctgccat tactggtgct gattgtcggc ccaaccgaat ttattaccaa
     4261 cagcatcatc aatgccatcg gcctgaccac gcaaaacttc ctgcaaatga gcttattcac
     4321 cgatccgctt ggcgatggtt catttacccg caactggacc gttttctact ggctgtggtg
     4381 gatctcatac acccctggcg tagcaatgtt tgtcacccgc gtttcccgcg gtcgtaagat
     4441 taaagaagtt acctggggac tgatcctcgg cagcaccgtc ggttgctggt tcttctttgg
     4501 cgtaatggaa agctatgcca ttcatcagtt tatcaatggc gtaatcaacg tcccacaggt
     4561 gctggaaaca ctgggcggcg aaacagctgt acagcaagtt ctgatgtcgt tgccagccgg
     4621 taaattgttc ctcgccgcat acctgggcgt gatgattatt ttccttgcct cgcatatgga
     4681 tgcagtggcc tacaccatgg cggcgaccag tacgcgtaat ctccaggaag gtgacgatcc
     4741 tgaccgtggg ctgcgtcttt tctggtgcgt ggtgatcact ctgatcccgc tttccatctt
     4801 gtttaccggt gcttcgctgg aaacgatgaa aaccaccgtc gtgctcacag cccttccctt
     4861 cctcgtcatt ttactggtga aagtcggcgg gtttattcgc tggctgaaac aggattacgc
     4921 cgacattccg gctcatcaag ttgaacatta tctcccgcag acaccggttg aagccctgga
     4981 aaaaacgcca gtgctccctg cgggaaccgt attcaaaggc gacaactgag cgccatcatc
     5041 ctaacgataa aggtatccct atgagcaatc tgagccctga ctttgtacta cccgaaaatt
     5101 tttgcgctaa cccgcaagag gcgtggacca ttcctgcccg tttttatacc gatcagaacg
     5161 cgtttgaaca cgaaaaagag aacgtcttcg ccaaaagctg gatttgcgtc gctcacagca
     5221 gcgaactggc gaatgccaat gattatgtga cgcgtgagat cattggcgaa agcatcgtgc
     5281 tggtacgcgg tcgtgataag gttttgcgcg cgttctataa cgtgtgtccg caccgtggtc
     5341 atcagttgtt gagcggtgaa ggaaaagcaa aaaatgtgat tacctgcccg tatcacgcat
     5401 gggcattcaa actcgatggc aacctggccc atgcacgtaa ctgcgaaaac gtcgccaatt
     5461 tcgatagcga caaagcgcaa ctggttccgg tgcgtctgga agaatatgcc ggattcgtct
     5521 tcatcaacat ggaccccaac gccaccagcg tagaagatca attacccggc ctgggcgcga
     5581 aagtgctgga agcctgcccg gaagtccacg atctgaaact ggcggcccgc tttaccaccc
     5641 gcacgcctgc caactggaag aacattgtcg ataactatct cgagtgctat cactgtggtc
     5701 cggcgcatcc aggtttctcc gactccgtac aggttgatcg ttactggcac accatgcacg
     5761 gtaactggac gctgcaatac ggtttcgcca aaccgtccga acagtcgttt aaatttgaag
     5821 agggtacgga tgcggcattc cacggtttct ggctgtggcc gtgcacgatg ctgaacgtca
     5881 ccccgatcaa agggatgatg acggtcattt atgaattccc ggtggattct gaaactaccc
     5941 tgcaaaacta cgatatttac ttcaccaatg aagagttaac cgacgagcaa aaatcgctga
     6001 ttgagtggta tcgcgatgtg ttccgtccgg aagatttacg tctggttgaa agcgtacaga
     6061 aagggctgaa atcgcgtggc tatcgtggtc aggggcgcat catggccgac agtagcggta
     6121 gtggcatttc cgaacatggt atcgcccatt tccataatct gctggcgcag gtgtttaagg
     6181 actaatgaca tcggcggcgg tattttccgc cgctgggctg atttttgatg gagtacagca
     6241 atgtcagact atcaaatgtt tgaagtacag gtgagccagg ttgaacccct taccgaacag
     6301 gtgaaacgct tcacgctggt ggcaaccgat ggcaaaccat tacctgcgtt taccggagga
     6361 agtcacgtca ttgtgcagat gagcgatggt gataaccagt acagcaatgc gtattcacta
     6421 ctgagttcgc cgcatgacac ctcttgttat cagattgccg ttcggctgga ggaaaactcg
     6481 cgcggcggtt cccgcttttt gcatcagcag gtaaaagtgg gcaatcggtt aacgatttca
     6541 acgcctaata acctgtttgc gctaattccc tcagccagaa agcatctgtt tatcgcgggc
     6601 ggtattggta tcaccccttt cctgtcgcac atggcagagc tgcaacacag cgacgtcgac
     6661 tggcagctac attactgctc gcgaaatcca gaaagttgcg catttcgtga tgagctagtc
     6721 cagcatccgc aggctgagaa agttcacttg catcactcat caaccggaac acgactggaa
     6781 ttagcgcgat tattggcgga tatcgaacct ggcacacacg tttatacctg tggtcccgag
     6841 gcgctaaatg aagcggtaag aagtgaagct gcgcgtctgg acatcgccgc cgatacgctg
     6901 cactttgagc aatttgctat cgaagacaaa accggcgatg cctttaccct ggtgcttgcc
     6961 cgttccggaa aagagtttgt ggtgccggaa gagatgacta ttttgcaggt tattgaaaat
     7021 aataaagccg cgaaagtgga atgtttatgt cgtgaagggg tatgcggaac ctgcgaaaca
     7081 gcaatactgg aaggtgaagc tgaccatcgg gatcagtatt ttagcgatga agagcgtgcc
     7141 agccagcaaa gtatgttgat ctgttgttcg cgtgcgaagg gtaaacgcct ggtgttggat
     7201 ttgtagtttg ccaaagccgg atgtggcgct gagcgcgccc agtccggctt cggaagattt
     7261 tactgcggat attcctgcaa taaattgtgt aatgcttccg ccatcagctc accacgccag
     7321 ccggaaatca gctccggcaa attgttctgc ggtttcagtt tccagtgcca gttcagcagt
     7381 tggttgattt gccgacgcga tgccagcaat tcggcgctga tcttatgcgt ttcgctcacg
     7441 tcagtaatca gcgacttaat cgctttaaac gctttacgat aacccggcat gtccatcagg
     7501 ttaagcatcg gctgcggtaa ggcctcttcc ggcaatgtct gcgctttttc caccagcgcc
     7561 agcagcgttt taccgtgaaa gcggatttcg ctaccggata aacccaggct gtccagttcg
     7621 cctaaacttc ccggcatata acgcgcgacc gaccacaaat gctcttcacg cacgacaaag
     7681 ttcaccgcca gatcacgctc acgagccttg cgcagtcgcc agtcggctaa cagttgcaga
     7741 caggccagtt ggcgtgtgcg taattgccag gcattggtca tatcacgcca ggcatcttcc
     7801 ggcgcaacga cttcctgacg acgcatttgc atcaggcggc attcatccag cgccgcaggt
     7861 agccagccgg aggcctccgt ttctaccata agcttggcgg tgatcggtaa cagataccag
     7921 acatcagccg ctgcgtattc acactgacgt tcggtcagcg gtctggccag ccagtcggtg
     7981 cgcgattcac tcttgtccag cgtaacgccg gaatactctt ccaccatgga agcgaacccc
     8041 catgacatcg ggcgtccgca gaaggcagca aggatttgcg tgtcaatcag gggttgtggt
     8101 aattcgccaa agacattgag gaacacttcc agatcttcac tgcctgcatg gagaaatttt
     8161 gtgatggacg gatcgcgcag gatcgctttc agcggtgacc agtcggtgat cccgagagga
     8221 tcaattaacg ccagatgctc accatcaaaa agttgaatta aacccagctg cgggtaataa
     8281 gtgcgcgtac gaacaaattc agtatccagg gctatcgccg gaaaggcacg gacggcttca
     8341 cacaaagaag ccagcgcatc gtccgtggta atcatttggt aattcaaatt gtattctctt
     8401 tagtgggcgt caaaaaaaac gccggattaa ccggcgtctg acgactgact taacgctcag
     8461 gctttattgt ccactttgcc gcgcgcttcg tcacgtaatt ctcgtcgcaa aatttttccg
     8521 acgttagatt tcggtaactc atcacgaaac tccaccagct tcggtacttt gtagcccgtg
     8581 agctgacggc ggcaaaaggt caccagtgac tcttcggtaa gcgatggatc ttttttcact
     8641 acgaagattt tcaccgcttc accactggag ccggaaggta cgccaacagc cgcgacttcc
     8701 tgtacgccag gatgctgcat gacgacatct tcaatctcgt tgggatagac gttaaaaccg
     8761 gaaaccagaa tcatgtcttt tttacgatcg acaatgcgca ggaacccttc ttcatccatc
     8821 accgcgatgt cgccggtgtg taaccagcca tttttgatga tttcatcggt agcatcggga
     8881 cgctgccagt aacccagcat cacctgcggt cctttgacac aaagctcacc cggttgacct
     8941 ggtggtactt cattatcatc atcatccacc agtttggctt ccgtcgacgg caccggcaaa
     9001 ccgatgctac cactatgata atcaatatca tatgggttaa cgctgaccag cggcgcacac
     9061 tcggtaaggc catagccttc cagcagatac tgtccggtca gtttcaccca acgctctgcc
     9121 accacttgct gcactggcat cccaccgcct gcggaaagat gcagactgga gaaatccagc
     9181 tgctggaact ctttattgtt cagcaacgca ttgaacaagg tgttaacgcc agtgatagcg
     9241 gtaaacggat atttcgctaa ctcttttacc aaccctggaa tatcacgcgg gttagtgata
     9301 agcaggttct gcccacccag ttcgataaac agcaggcagt taatggtcag ggcaaaaatg
     9361 tgatacagcg gcagtgccgt tactaccagt tctttacctg gatgcagcaa cggcccatag
     9421 gtggcgttga cctgttccag gttcgccagc atattgcggt gagtcagcat cgcgcctttc
     9481 gccacacctg tggtgccgcc ggtgtattgc aggaaggcta aatcttccgg caccagttcg
     9541 ggtttgacgt actgcatccg gtagccgtta tgcagtgcgc tacgaaatga aatggcatct
     9601 ggcagatggt atttcggcac caaacgcttg atgtatttaa caacgaaatt gactaccgtg
     9661 ccttttgccg tagatagctg atcgcccata cgggtcagaa ttacgtgctg aacggcggtt
     9721 ttatcaacca ctttttccag tgtgtgagca aagttagaca cgataacaat cgccgatgcg
     9781 ccgctatcgt taagctgatg ctcaagctca cgcggggtat acaacgggtt aacgtttacg
     9841 acgatcatcc cggcacgcaa aatgccaaac agcgccaccg gatattgcaa taaattaggc
     9901 atcatcaacg caacgcgatc gcctttcttc agccccaacc cttgttgcaa ataagcggca
     9961 aacgcgcgac tgcgttcttc cagcttgcgg aaggtcatca cctcccccat attcacaaac
    10021 gcaggctgat cggcgtagcg cgcgaccgac tgctcaaaca tatctaccag agattgataa
    10081 cggtcagggt tgatctccgt cggaacgtcc gcgggataac ggttaagcca aaccttcttc
    10141 aattcttcac ctctaaaatg cgtgttcgtc gtcatcgcaa ccccaaatga tatacatgct
    10201 gttaacataa tattaactca tcataccagc ttgataatta cccaacgaaa aggttgcgaa
    10261 gcgcgtcact atttattttt atctttaccg taagaatgca gaaacagcgg accagccgct
    10321 gtttcttttt ctttaaaaac aagcaattat tcagttacaa ctgtttgtac tctcgcggga
    10381 ccgggattat accagcccca tccgccgtag ccatagggcc agccacgacc gccataaaac
    10441 catggatcaa tcggctgagg cggcataatc acctgctggg ttaaatgcca acgtttgtaa
    10501 cccgttactt gcatcaccat aaatttatag ggcgtattgc cgattttgcc gtcaaccgca
    10561 ccagtgattg gcccgactac cgtaaccagt tgtccacgga aatccaccgg gtccagaaaa
    10621 ccgttcacat cggcataaat gcgaccgcga gaaggttctc ccagcgtcgg tctggctccg
    10681 ctgtccagcg gtacggtagc aatttccagg cgagttttcc cttgctggtt ttgtaccgca
    10741 accactttgc caccaaagcg tgcctcctga ccaacgtaca gctgcggcgc actcatcacc
    10801 cgaactaaat cttgctgcgg cgtgggactg ctgcctttaa tggcgtccgg cacagtgaca
    10861 caaccgctaa gcattagcgc aaacgtacct gccagtatgc ctttgataac attcttttga
    10921 accgccatgg tgcgactcct ttttttctca gggcatactc ttaagattca ttctttgccc
    10981 ggaagtttct tccatgcgac gttgttacgt aaataaaccg gttcggcatg ttccaccgcc
    11041 accgttttac cctcagcaaa catctgacac gcaatcggca gcatatcttc agcagcaggc
    11101 agtaacactt cgccatcgcg caaaaccagc ccgctctctt taccgagatc cggccaagct
    11161 tgccagcccg tccctaccgt cacccattcg ccggaaagct gttgcattcg ttcatggacg
    11221 atttcgggtt tgagtacggc ttcggtttct tcaccgtgcc agataccgtt ttcatcacgc
    11281 tgatattcgg cccagtaaac ttcgcccatt cgcgcgtcaa tggctgccag cacgcgggtt
    11341 gcgccgtttt tgcgccacgc cccttgcgcc atcgtcatta gcgtggagac gccaatcatc
    11401 ggtaattccg cgccaagcgc cagcccttgc gcgatgccaa taccaatgcg cacgccagta
    11461 aagctaccgg ggccgcgccc gtaagccaga gcgttaatat cagtcaggga agttccgctg
    11521 gtggtcagga tatcctgcac catcggtaag attcgttgag tatgttcacg agggcaaagc
    11581 tcaaaatgag cgttgacagt accgtcgttc cacagggcga cagagcaggc ctctgtcgcg
    11641 gtatcgatag ccagaattcg catgggtctt cgtgcttaga tcaataaaaa ggcgcgcatc
    11701 ataccatact ccgtaacaaa ttacctggag gatggtatcg caaggaaacg aaccgcacgg
    11761 gcaatgtcac gggtgcgtgg cgcgggcggc agactggcga gaaacgtcgc gccgtaagga
    11821 cgcatcacca gccgattgtc acaaatcacc agcacgccac gatcgtcggc gtcgcgaatc
    11881 agtcgcccta ccccctgttt gagagtaatg acggcatctg gtagttgcac ttcatcgaac
    11941 gggtcgccac cgcgcaaacg acaatcttcc atgcgcgctt ttaacagtgg atcatccggc
    12001 gaggtaaacg gcaatttgtc gataattacc aatgacaatg tatcgccacg cacgtccacc
    12061 ccttcccaga aactgctggt tgccacaaga agcgcattac cggcgctgac aaattgctgc
    12121 aacagttgcc ctttgctggt ttccccctgc aacaatacag gaagcgtcat ggtagcgcgg
    12181 aactgctcgg ccagatcgcg catcatggcg tgcgaggtac aaagcataaa acaacgaccg
    12241 ttgttagctt cgatgatcgg tcgcagcatt gccgctaact ggcgagcaga acctggctgg
    12301 ttggtttgcg gcagattgcg cggcacacag agtaacgcct ggcggctgta atcaaatggg
    12361 ctgggcaaca gcaacgactc ggcctgttcg atgccaagcc gcgaggtgaa atgatgcaga
    12421 tcgtcgttca ccgacagcgt tgctgaggtg aagatccagc taccgggttt ttgcgccatt
    12481 aactctttga atttatccgc cacgctgagc ggcgtgagag ccagagtaaa atggcgcgaa
    12541 gtgcattcgt accagtagct gtagcccggc tgattgatct cttttagccg cttcagccgt
    12601 gtgcgataca acgtagcgcg ctcaaatgcc gcatccagca aggcggaacg ccccagtgac
    12661 agtttcgcca cgtcataaca aagttccagg gtgtcatcga gcagtaaaaa tgcccgctga
    12721 atttgcggat tagctaacag ctcacgcagg ttaccgcgat aacctggctc accgagttgc
    12781 agacgaaaat cctgcgcact ctgggcaaga cgatcagcgc acttttgtaa ctgctgggtg
    12841 tcttttaatt cggtgcggta ggcgatggtg atgtcttttg ccaggtcgag cagttgtcga
    12901 ctggagagtg actgaccaaa atactggctg gcaatgtccg gtagctggtg ggcttcgtcg
    12961 aagatcatga cgtccgcttc cgggatcagt tcgccaaatc cactctcttt aaccaccata
    13021 tccgccagaa agagatgatg gtttaccacc accacatcgg cgtccatcgc ttttttacgt
    13081 gctttgacca caaagcaatc tttatacatc gggcagtcgc tgccaagaca gttgtcgttg
    13141 gtgctggtaa ccagcggcca cgcctgtgaa tcttccgcca cgctgacgca ggtgctgata
    13201 tcaccatcga ctgtttgatt agaccaggag cgcagcagga tcacatcgct taagatttgt
    13261 accggcagat cgccccccgc cagcgcctgc tgttcgagac gttcgaggca gaggtagttt
    13321 gagcgccctt tcagcagcgc cacgttgccc gtatatttca atgcctttga gactgttggc
    13381 aaatcgcggc tgtagagctg atcctgcaac gcttttgagc cggtcgagat aatgactttc
    13441 tttttcgccc gcagcgcagg agccaggtaa gcgtaggttt tgcccgtacc ggttcctgct
    13501 tccaccacca gcggctggcc tttttctatc gcctgggtga cggctaccgc catctgtcgc
    13561 tgtggttctc gcggcttaaa gcctggtatc gctttcgcca gctgaccgtc tggtgcaaaa
    13621 tcgtccgtca cactaccccc tgttgatttg aacagggatt atgtcaggat gagggcgctt
    13681 tcgccagttg aagtggtgac ggcgacctca cattgtggca gtctttgcag cacgaaaatg
    13741 gaagtataat gaggacaaaa tgatgactat cgttcgtatc gatgctgaag cccgctggtc
    13801 tgatgtagta atccacaaca acacgctcta ctacactggt gtaccggaaa acctcgacgc
    13861 cgatgccttt gagcaaaccg ccaacacgct ggcacagatt gacgccgtgc tggaaaaaca
    13921 gggcagcaat aaatcgagca ttctggatgc caccattttc ctggccgata aaaacgactt
    13981 cgcggcgatg aataaagcgt gggatgcctg ggttgtcgcg ggtcatgcgc cggtgcgctg
    14041 cacggtacaa gcgggtttga tgaacccgaa gtataaagtt gaaattaaga ttgtggctgc
    14101 ggtgtaagct ttatcgaagc aaaataagtc agacgataat ttatcgataa tactggtcgg
    14161 ttttacataa atcgaccaga gaataagata tt
//
LOCUS       DAPHBJ010000109        14126 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.110, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000109 DAPHBJ010000000
VERSION     DAPHBJ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.110"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            7..720
                     /gene="gspC"
                     /locus_tag="RHZ76_002908"
     CDS             7..720
                     /gene="gspC"
                     /locus_tag="RHZ76_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HDV2813512.1"
                     /translation="MNYFHTTIVKNGQIINQPTNAFQSDFSLAALWRNENHAGVKDAN
                     PVAVNQETPKLSIALNGIVLTSNDETSFVLINEGSEQKRYSLNEALESAPGTFIRKIN
                     KTSVVFETHGHYEKVTLHPGLPDIIKQPDSESQNVLADYIIATPIRDGEQIYGLRLNP
                     RKGLNAFTTSLLQPGDIALRINNLSLTHPDEVSQALSLLLTQQSAQFTIRRNGVPRLI
                     NVSVGELTGMNGLRHERTQ"
     gene            704..2656
                     /gene="gspD"
                     /locus_tag="RHZ76_002909"
     CDS             704..2656
                     /gene="gspD"
                     /locus_tag="RHZ76_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HDV2813513.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNARKPSSSGAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPIFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYRSLSKEKYTRYRQEQQQRIDGKSK
                     ALVGSEDLPVLDENTFNSHAPAPSSR"
     gene            2666..4147
                     /gene="gspE"
                     /locus_tag="RHZ76_002910"
     CDS             2666..4147
                     /gene="gspE"
                     /locus_tag="RHZ76_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HDV2813514.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHKALIKNGLQKVISGDTSWDEVMRVASATLESEA"
     gene            4144..5340
                     /gene="gspF"
                     /locus_tag="RHZ76_002911"
     CDS             4144..5340
                     /gene="gspF"
                     /locus_tag="RHZ76_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HDV2813515.1"
                     /translation="MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNKRLGDVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTTVAIGVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQRTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLNNLEIRQRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            5350..5787
                     /gene="gspG"
                     /locus_tag="RHZ76_002912"
     CDS             5350..5787
                     /gene="gspG"
                     /locus_tag="RHZ76_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HDV2813516.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     AVSDIVALENALDMYKLDNHHYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            5795..6304
                     /gene="gspH"
                     /locus_tag="RHZ76_002913"
     CDS             5795..6304
                     /gene="gspH"
                     /locus_tag="RHZ76_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HDV2813517.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDVANTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESQND
                     WDEELSIHLQPFKPDDSNQPQVVILADGQITPFSLLMANAGTGEPLLTLVCSGSWPLD
                     QTLARDTRP"
     gene            6301..6678
                     /gene="gspI"
                     /locus_tag="RHZ76_002914"
     CDS             6301..6678
                     /gene="gspI"
                     /locus_tag="RHZ76_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HDV2813518.1"
                     /translation="MNKQSGMTLLEVLLAMSIFTAVALTLMSSMQGQRNAIERMRNET
                     LASWIADNQLQSQDSFGEENTSSSGKELINGEEWNWRSDIHSSKDGTLLERTITVTLP
                     SGQTTSLTRYQSIDNKSGQAQDD"
     gene            6671..7258
                     /gene="gspJ"
                     /locus_tag="RHZ76_002915"
     CDS             6671..7258
                     /gene="gspJ"
                     /locus_tag="RHZ76_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HDV2813519.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPLAPLSEAQTL
                     LTVHWYLRNHTLYRAVRTSVDGRKDQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     QTQQYGGLQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            7251..8234
                     /gene="gspK"
                     /locus_tag="RHZ76_002916"
     CDS             7251..8234
                     /gene="gspK"
                     /locus_tag="RHZ76_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HDV2813520.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTLSFHSQLQRTRQVNHH
                     LQRQYDIELAEKLALASLTQDVKDNDRQTTLQQYWAQPQQLQLEDGNTVKWQLRDAQH
                     CFNLNALAKISDDPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDVDDSPRF
                     HGAEDSFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTTELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKRHLFPYSRYFTLSTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            8249..9412
                     /gene="gspL"
                     /locus_tag="RHZ76_002917"
     CDS             8249..9412
                     /gene="gspL"
                     /locus_tag="RHZ76_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HDV2813521.1"
                     /translation="MPESLMVIRSSSTLRKHWEWMTFSADSVSSVHTLTDDLPLESLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDNTQDWHWTVVDK
                     QNESVEVIGIQSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQTSWLIR
                     SAAHAFNELDEHWLQHLAAQFPPENMLCYGVVPHGVAAANPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGIFRKQKTVSKSGKWLARLAVSCLVLAILSFVGSRSIALWHTLKIEDQL
                     QQQQQETWQRYFPQIKRTHNFHFYFKQQLAQQYPEAVPLLYHLQTLLLEHPELQLMEA
                     NYSQKQKSLTLKMSAKSEANIDRFCELTQSWLPMEKTEKDPVSGVWTVRNSGK"
     gene            9409..9870
                     /gene="gspM"
                     /locus_tag="RHZ76_002918"
     CDS             9409..9870
                     /gene="gspM"
                     /locus_tag="RHZ76_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HDV2813522.1"
                     /translation="MIKSWWAEKSTSEKQIVAALAVLSLGVFCWLGVIKPIDTYIAEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLAITLENGPDNTL
                     TIHPVTAPLENVSRWLTTAQVTYGIVIEDLQFTLAGNEEITLRHLSFREQQ"
     gene            9870..10547
                     /gene="gspO"
                     /locus_tag="RHZ76_002919"
     CDS             9870..10547
                     /gene="gspO"
                     /locus_tag="RHZ76_002919"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase GspO"
                     /protein_id="HDV2813523.1"
                     /translation="MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHF
                     RQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGALFLYFCFVLTLSVIDFRTQLLPD
                     KLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
                     LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLG
                     YLAWISY"
     gene            complement(10576..11052)
                     /gene="bfr"
                     /locus_tag="RHZ76_002920"
     CDS             complement(10576..11052)
                     /gene="bfr"
                     /locus_tag="RHZ76_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HDV2813524.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREEG"
     gene            complement(11124..11318)
                     /gene="bfd"
                     /locus_tag="RHZ76_002921"
     CDS             complement(11124..11318)
                     /gene="bfd"
                     /locus_tag="RHZ76_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HDV2813525.1"
                     /translation="MYVCLCNGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVR
                     AAREVMEDELMQLPEFKESA"
     gene            complement(11487..14123)
                     /gene="chiA"
                     /locus_tag="RHZ76_002922"
     CDS             complement(11487..14123)
                     /gene="chiA"
                     /locus_tag="RHZ76_002922"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001491351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HDV2813526.1"
                     /translation="MPALAMEAWNNQQGGNKYQVIFDGKIYENAWWVSSTNCPGKAKA
                     NDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSNTPASNTPANGGSATPAQGT
                     VPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNCPGDAKGNDASNPWRYVRAA
                     TATEISETSNPQSCTSAPQPAPDVKPAPDVKPAPDVQPAPADKSNDNYAVVAWKGQEG
                     SSTWYVIYNGGIYKNAWWVGAANCPGDAKENDASNPWRYVRAATATEISQYGNPGSCS
                     VKPDNNGGAVTPVDPTPETPVTPTPDNSEPSTPADSGNDYSLQAWSGQEGSEIYHVIF
                     NGNVYKNAWWVGSKDCPRGTSAENSNNPWRLERTATAAELSQYGNPTTCEIDNGGVIV
                     ADGFQASKAYSADSIVDYNDAHYKTSVDQDAWGFVPGGDNPWKKYEPAKAWSASTVYV
                     KGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNSRPWKPLGKAQSYSNEELNNAPQ
                     FNPETLYASDTLIRFNGVNYISQSKVQKVSPSDSNPWRVFVDWTGTKERVGTPKKAWP
                     KHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVVSKDANTCLPTWGTAYGMQNYAQ
                     YSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDLMQHYYDIVDNLNLRVLDFDIEG
                     TWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTLPILPTGLTPEGMNVLSDAKAKG
                     VELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSAIANLHSQLKGLHPNKSDAEIDA
                     MMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLGMVGIWSIARDLPGGTNLSPEFH
                     GLTKEQAPKYAFSEIFAPFTKQ"
BASE COUNT         3624 a         3649 c         3377 g         3476 t
ORIGIN      
        1 tttaatgtga attatttcca tacaactata gttaaaaatg gacagatcat taatcaacca
       61 acaaacgcat ttcagtctga tttcagcctt gccgctttat ggcgtaacga aaatcatgct
      121 ggggtaaaag atgcaaatcc cgtggcagtt aatcaggaaa cacctaagct ctctatagcg
      181 cttaatggca ttgtactcac atcgaatgat gagacttctt ttgtcttaat taatgagggg
      241 agcgaacaaa agcgatactc cctcaacgaa gcccttgagt cagcgccggg aacgtttatt
      301 agaaaaataa ataaaaccag cgttgtgttt gaaactcacg ggcattacga aaaagtaact
      361 ctccatccag ggctacctga catcatcaag cagcctgatt cagaaagtca aaatgtactc
      421 gctgattata ttatcgccac gcctatacgt gatggagaac agatatacgg gcttcgattg
      481 aaccctcgta agggcctcaa tgcatttacc accagcttgc tacaaccagg ggatatcgcc
      541 ctcaggatca ataatctttc gctgacccac cctgatgagg tttcacaggc attaagttta
      601 ctgctaactc aacaaagtgc tcagtttaca attcgtcgca acggcgtacc ccgcttgata
      661 aatgtttccg tcggggaact tacaggaatg aatggactgc gtcatgaaag gactcaataa
      721 aatcacctgc tgcttgctgg cagcactact catgccttgt gcaggacacg ctgagaacga
      781 acaatacggc gcgaacttca ataacgccga tatccgccag ttcgtggaaa tagtgggtca
      841 gcatcttggc aaaacgatcc tgatcgaccc ttcggtacag ggaaccattt ccgtacgcag
      901 taatgatacg tttagccaac aggagtacta ccagttcttt ttaagtattc ttgatcttta
      961 cggttattcc gtgatcacgc tggacaatgg ttttctgaaa gtggttcgct cagctaatgt
     1021 aaaaacatcg ccagggatga ttgctgacag ttctcgtcca ggcgtaggtg atgagttggt
     1081 cacccgaatt gtaccgcttg agaacgttcc tgctcgtgac ctggcccccc tgctccgcca
     1141 gatgatggat gcgggtagcg tcggtaatgt tgtgcattat gaaccctcca acgttcttat
     1201 tctgaccggt cgtgcctcca ccattaataa actgattgaa gtcataaagc gcgttgatgt
     1261 catcggcaca gagaagcagc aaattattca tctggaatat gcgtcagcgg aagatctcgc
     1321 cgagattctt aatcaattaa tcagcgaaag ccacggtaaa agccagatgc cagccctcct
     1381 ctccgcgaag attgtggcgg ataagcgaac caactctctt atcatcagtg gaccggaaaa
     1441 agcacgccag cgcatcactt cattactgaa aagccttgat gtcgaagaga gcgaggaagg
     1501 aaatacccgg gtttattacc tgaaatatgc taaagccacg aatctggtgg aagtgctaac
     1561 cggtgtttcc gaaaagctga aagatgaaaa agggaatgcg cgtaagccct cctcttctgg
     1621 cgcgatggat aacgtcgcca ttaccgccga tgaacagact aactctctgg tcattaccgc
     1681 tgaccagtcc gtccaggaaa aactcgccac ggtaattgcg cgtctggaca ttcgccgtgc
     1741 acaggtgctg gttgaggcaa tcatcgttga agttcaggat ggaaatggac taaacctcgg
     1801 cgtgcaatgg gcgaataaaa acgttggcgc acagcaattt accaataccg gattaccgat
     1861 ttttaacgct gcgcaaggtg tggctgatta taaaaagaat ggtgggatca ccagcgcgaa
     1921 tcctgcctgg gatatgttta gcgcctacaa tggcatggcc gcaggcttct tcaatggcga
     1981 ctggggagta ctgcttaccg cgctggccag taacaataaa aatgacatcc tcgccacccc
     2041 aagcatcgta acgctggata ataaactcgc gtccttcaac gtggggcagg atgtgccggt
     2101 gctatccggg tcacagacca cttcagggga taacgtcttt aataccgtcg aacgcaaaac
     2161 ggtggggaca aaactcaaag ttactccgca ggtcaatgaa ggcgacgcgg tgttgctcga
     2221 aatagagcag gaagtctcca gcgttgactc ttcctctaac tcgacgctcg gcccgacgtt
     2281 taatacccgt actattcaaa acgccgtgct ggtcaaaacc ggtgaaacgg tggtcctggg
     2341 cggattgctg gatgattttt ctaaagagca agtgtcaaag gttcctctgc ttggcgatat
     2401 tcctttagtg gggcaactct tccgctatac ctccaccgag cgcgctaaac gcaacctgat
     2461 ggtatttatc cgtccgacga ttatccgtga cgatgatgtt tatcgctcac tgtcaaaaga
     2521 gaaatacacc cgttaccgtc aggagcaaca acagcggatc gacgggaaat caaaagcgct
     2581 ggttggctcg gaagatttgc cggtgctgga tgaaaacacg ttcaacagtc acgcccctgc
     2641 gccatcgtca cggtgaggca ttcatatgag aattcactca ccgtaccccg ccagttgggc
     2701 gctggcacaa cgaattggtt atctctattc agagggcgag attatttatc tcgccgatac
     2761 gccattcgag cggttactcg atattcaacg tcaggttggc cagtgccaga ccatgaccag
     2821 cttgtcacag gctgattttg aagctcggct ggaagcggta ttccatcaga ataccggtga
     2881 gtcgcaacag attgcgcagg atatcgatca atccgtcgat cttctctcgc tttcggaaga
     2941 gatgcccgca aatgaagatc tcctgaatga agattcagcg gcaccggtta tccgcttgat
     3001 caatgcgatt ttgagtgagg ccatcaaaga aaccgcctct gatatccaca ttgaaaccta
     3061 tgaaaaaaca atgtcgatcc gttttcgcat cgacggcgtt ttgcggacaa ttttacagcc
     3121 aaacaaaaaa ctggcggcac tgcttatctc ccgaattaag gtcatggctc gtcttgatat
     3181 cgccgaaaaa cgtattccac aggatggaag aattagtttg cgtatcgggc gacgtaacat
     3241 agatgtccgc gtatccacac tgccgtccat ctatggtgaa cgcgccgtac tccgcctgct
     3301 ggataaaaac agcctccagc tttcattgaa caacctgggg atgacggcag cggataagca
     3361 ggatttagaa aatctcattc agcttccgca cggtattatc ctggtgacag ggccgacagg
     3421 ctccggtaaa agcaccacgc tctacgccat cctttcggcg ctgaatactc ccggccgcaa
     3481 tattctgacg gtagaagatc ccgtggaata tgagctggaa ggcattgggc aaacgcaggt
     3541 gaatacccgt gtggatatgt ctttcgctcg cggcctgcgc gccatacttc gccaggaccc
     3601 ggatgtcgtc atggtggggg aaattcgtga tacagaaacc gcgcagattg cggttcaggc
     3661 ctcgctcacc ggccatctgg tactctcaac actccacact aacagtgcat caggcgcagt
     3721 gacccggctc cgcgacatgg gcgtcgaatc attcctgctt tcgtcttccc tggcagggat
     3781 tatcgcgcaa cgtctggttc gtcgcctgtg tccgcaatgc cgacaattca cgcccgtatc
     3841 accccaacaa gcgcagatgt ttaaatatca tcagctcgcg gtgacaacaa ttggcactcc
     3901 cgtaggctgc cctcattgcc atcaatccgg ctatcagggg cgcatggcga tccacgaaat
     3961 gatggtggtg acgccggaat tacgggccgc tattcatgaa aatgtggatg aacaagcact
     4021 ggagcgacta gtccggcaac aacacaaggc cttaatcaaa aatggcctgc aaaaagtgat
     4081 aagcggtgac acctcctggg atgaggttat gcgcgtcgcc agtgccacgc tggagagcga
     4141 agcatgaatt atcgctatcg cgccatgacc caggatggtc aaaaattgca agggatcatt
     4201 gatgctaacg atgaacgtca ggcacgactg cggctgcgtg aagaagggct tttcctgctg
     4261 gatattcgcc cccaaaaaag ttcgggagta aaaacacgtc gcccgaggat cagccatagt
     4321 gaactgacgc ttttcacccg gcagttggca accttaagcg cagcggcatt acccctggaa
     4381 gagagccttg ccgtaatcgg tcaacaaagc agtaataaac gactgggtga cgtgttaaat
     4441 caggtacgca gcgccatcct tgaagggcat cccctttccg atgcattaca gcattttccc
     4501 acgcttttcg attcgctcta tcgtaccctg gtaaaagcgg gcgaaaagag cgggctgctg
     4561 gccccggtgt tggaaaagct ggctgattac aatgaaaacc ggcagaaaat ccgcagcaag
     4621 ctcattcagt cactgatcta cccctgtatg ctcactacgg tggcgattgg ggtcgtgatt
     4681 attctcctca ctgctgtcgt gcccaaaatt accgaacagt tcgtgcatat gaagcagcaa
     4741 ctgccgctga gtacacgcat tcttttaggt ctgagcgaca cgttgcaacg taccggcccg
     4801 acattattag cgacagtgtt tattgtcgct gtaggtttct ggctctggtt aaaacgcggc
     4861 aataaccgcc accgttttca tgccatgttg ctgcgcgttg cgctcatcgg cccgctgatt
     4921 tgcgccatta acagcgcacg ctatctccgc actttaagta ttttgcaatc cagcggcgtc
     4981 cctctgctgg atgggatgaa tttgtccacc gaaagcctca acaacctcga aattcgccag
     5041 cgtctggcaa atgcggcaga gaacgttcgc cagggtaaca gcattcatct ttcgctggaa
     5101 caaaccgcaa ttttcccgcc gatgatgctc tacatggtgg cctctggcga aaaaagcggg
     5161 cagctcggca cattaatggt cagagccgca gataaccagg agacactcca acaaaatcgg
     5221 atcgccttaa cgctctccat cttcgagcca gcactcatta ttacgatggc actgatcgtc
     5281 ctgtttattg tcgtgtcggt actccaacct cttcttcaac ttaactcaat gattaattaa
     5341 ggaaagcata tgcgcgcaac ggataagcaa cgcggtttta cattactgga aattatggtg
     5401 gtgatcgtca ttatcggcgt acttgccagc ctggtggttc ctaacctaat gggcaataaa
     5461 gaaaaggcgg ataagcaaaa agccgtcagc gatattgtgg cgctggaaaa cgcccttgat
     5521 atgtacaaac tcgacaatca tcactatcca accacaaatc aggggcttga atccttagta
     5581 gaagcgccga cactgccacc gctggccgca aactataaca aggaaggtta tatcaagcgt
     5641 ctgcctgccg atccctgggg caatgattat gtcctcgtta atcctggtga acatggtgca
     5701 tacgatctgc tttcagcagg gcccgatggt gaaatgggaa ccgaggacga catcaccaac
     5761 tggggtttga gcaagaagaa aaagtaagtc agcgatgaat cagcaacgcg ggtttaccct
     5821 gctggagatg atgctggtgc tggcgctggt cgctatcacg gcaagcgtgg tgctcttcac
     5881 atatggccgg gaggatgtcg ccaacactcg tgcgcgcgaa acagcggcgc ggtttaccgc
     5941 tgcacttgaa ttagccatcg accgcgccac gctcagtggt cagcctgtcg gtatccattt
     6001 ctctgattca gcatggcgca ttatggtacc gggcaaaaca ccatcagcct ggcgctgggt
     6061 tccattacaa gaagatgccg cagacgagag ccaaaatgac tgggatgaag aactctcaat
     6121 tcatcttcag ccatttaaac ctgatgacag caaccagcca caggttgtga ttctggccga
     6181 cggacaaatc acgcccttct ctttgttgat ggctaacgcc gggaccggtg agccgctcct
     6241 gacgttagtc tgttcaggct catggcccct ggatcaaacg ctcgccagag atacccgccc
     6301 atgaacaaac aatcagggat gacactgctt gaagtcttac tggcgatgag tattttcacc
     6361 gctgttgctc tgactttaat gagcagtatg caagggcaac gaaatgccat cgagcgaatg
     6421 cgtaacgaga cgctggcatc gtggatagcg gataaccagc ttcagtcaca agattcattc
     6481 ggcgaggaaa acacgtccag ttctggcaaa gagttaataa atggcgaaga gtggaactgg
     6541 cgcagcgata tccactcaag caaagacggc acgcttctgg aacgcaccat aacggtgaca
     6601 ttacccagcg gtcagacaac ctcgcttacg cgttatcaaa gcatcgataa taaatccggg
     6661 caggcacaag atgattaatc gccagcaggg tttcacattg ctggaggtca tggcggcgct
     6721 ggcgattttc tcaatgctta gcgtgctggc gtttatgatt ttttcgcaag cttccgagtt
     6781 gcatcaacgc tcacagaaag aaatccagca gttcaatcag ctacagcgca ccatcaccat
     6841 tcttgataac gatctcctgc aactggtcgc ccggaggaat cggagtacgg acaaaatcat
     6901 ggtgttgggt gaagaagcta tttttaccac gcaaagtcgc gatccgctgg ctcctctcag
     6961 tgaagcacaa accttactca ctgttcactg gtatctgcga aatcatacgc tctaccgtgc
     7021 tgttcgtact tctgtggatg gcaggaagga tcaacccgcc caggcgatgc tggaacatgt
     7081 cgagagcttt cttctggaaa gtaacagcgg ggaaagccag gaacttcctc tctcagtcac
     7141 gttacatctg cagactcagc aatatggggg gttacagcgc cgttttgcat tacctgagca
     7201 gttagcgaga gaagagtcgc cagctcagac gcaggccggg aataataacc atgaataatg
     7261 aacaacgcgg cgtcgcgctg ctcattgtac tcatgttact ggcgctcatg gctgctctgg
     7321 cagcagacat gacgctcagt tttcacagcc agcttcagcg aacccgccag gtaaaccatc
     7381 atctgcaacg gcaatatgat attgaactgg cggagaaact tgcgcttgcc agcctcaccc
     7441 aggacgttaa agataacgac agacagacca cgttgcagca gtactgggca cagccacaac
     7501 agttgcagct ggaagatggc aatacagtga agtggcagtt acgcgatgcc cagcactgtt
     7561 ttaatctcaa tgcgttagca aaaatctctg acgacccgct ggcttcccct gatttccccg
     7621 cccaggtatt cagtgcgctg ctgattaacg ccggtatcga tcgcggaaac accgatgaga
     7681 tcgtgcaatc aatcgctgac tatattgatg tcgatgactc accacgcttt cacggcgcag
     7741 aagatagctt ttaccaaagc cagacgccgc cccggcatag tgccaatcag atgctttttc
     7801 tgactggcga attacgtcag ataaaaggca taaccgaaaa catctatcag cggcttatcc
     7861 cgtatgtctg cgttctcccg accacggaac ttagcatcaa tcttaatatg ctaacggaga
     7921 acgatattcc gctcttcaga gcgttattcc tcaataacat cacggatgct gacgctcggg
     7981 tcttattgca aaaaaggccg agggaaggct ggctaaccac ggatgcattt ctttactggg
     8041 cgcagcagga tttctccggt gtgaagccac tggttgctca ggtgaaaagg catctctttc
     8101 cctatagccg ctattttacg ctgagtacgg aaagtatcag cgacgaacaa tcccagggat
     8161 ggcaaagcca tatttttttc aaccgtaaac agcagagcgc ccaaatctat cggcgtacgc
     8221 tgcaactcta ctaatcataa ggatcgtgat gcctgaatcg ttgatggtca tacgttcttc
     8281 ctcgacgctg cgtaagcatt gggaatggat gacgttctcc gcagatagcg tatcttcggt
     8341 acatacgctc acggacgatc ttccgctgga gtcgctggcc gatcagcccg gtgccggaaa
     8401 tgttcatctt ttgatcccac cagagggcct tttatatcgt tccctcacgc tgcccaacgc
     8461 caaatacaaa ctcaccgcac aaacattaca atggctggca gaggagacgt tacccgacaa
     8521 cacccaggac tggcactgga cggttgtcga taaacaaaac gaaagtgtcg aggtgatagg
     8581 cattcagtcg gagaaactca gtcgctatct tgagcgttta catactgccg gactcaacgt
     8641 gacgcgggtt ttgccagatg gctgctatct tccctgggaa gtagatagct ggactctggt
     8701 taaccagcaa acaagctggc tcattcgcag tgccgcacat gccttcaacg aactggatga
     8761 acactggtta cagcatcttg ctgcccagtt tccgcctgag aatatgcttt gttatggtgt
     8821 agtacctcac ggcgttgcag cggccaatcc acttatccag catcctgaaa taccatcact
     8881 gagtttgtat tctgccgata ttgcattcca gcgctatgac atgctgcatg gtatttttcg
     8941 taaacagaaa actgtaagca aatcgggaaa atggctcgcc cgcctggcag taagctgtct
     9001 cgttctcgcc attctttctt ttgttggcag ccggagcatc gcgctctggc atacgcttaa
     9061 gattgaagat caacttcagc aacaacagca agaaacctgg caacgttatt tcccgcagat
     9121 caaacgcacc cacaactttc atttttactt taagcagcaa ctcgctcagc aatatcctga
     9181 agctgtaccg ttgctctatc atttacaaac acttctgctg gaacaccctg aactgcagtt
     9241 aatggaggcg aactatagcc agaagcaaaa gtcgctgacc ttaaaaatga gtgctaagag
     9301 tgaagccaat atcgatcgtt tctgtgagtt aacacagtcc tggcttccga tggaaaaaac
     9361 agaaaaagat ccggtcagtg gtgtatggac agtaaggaac tcagggaaat gattaaatca
     9421 tggtgggcag aaaaatccac atcggaaaaa cagatcgttg cggcactggc ggttctctcg
     9481 ctcggcgttt tttgttggct tggggtgata aaaccgatag atacctacat tgcagagcat
     9541 cagtcccacg cgcaaaaaat taagaaggac atcaaatgga tgcaggatca agcgagcacc
     9601 catggtttac tcggtcatcc tgcgcttacg cagcccatca aaaatatcct tctcgaggaa
     9661 gcaaaacgtg agaatttggc tatcacgctg gaaaacggcc cggacaatac tctgacaatt
     9721 catcctgtca cagctcccct cgaaaacgtc tcccgctggc tcactacggc acaggtaaca
     9781 tacggtattg ttattgaaga ccttcagttt actcttgccg gaaacgaaga gatcaccctc
     9841 aggcatttgt ccttcaggga gcaacaataa tgacaatgct actgccgctc ttcattctgg
     9901 ttggttttat tgcaggttat tttgttaatg ccatcgccta tcacctctcg ccgttggagg
     9961 ataaaacggc gttaacgttt cgccaggtat tggttcattt caggcaaaaa aaatatgcct
    10021 ggcatgatac agtgcccctg atactttgtg ttgctgctgc tatcgcctgc gccctggcac
    10081 ctttcacgcc catcgtgact ggcgcactct ttctctattt ctgtttcgta ctcacgctca
    10141 gtgttattga ttttcgcact cagctcctgc ccgacaaact caccttaccg ctgctctggc
    10201 ttggcttggt atttaatgcg cagtatggat taattgattt acatgatgcg gtttacggcg
    10261 cggtagcggg ctatggggtg ctgtggtgtg tttactgggg cgtctggtta gtttgtcaca
    10321 aagagggatt gggctacggt gatttcaagc tactggccgc tgcaggcgca tggtgtggct
    10381 ggcaaacgtt gccaatgata ctgctgattg cctcgctggg tggcattggt tacgccatcg
    10441 tttcacaact tctgcaacgc cggactataa ccactatcgc attcggcccg tggcttgcgc
    10501 tcggcagcat gataaacctg gggtatctgg cctggatctc ttattaaccg ggagggttct
    10561 ccctcccgac acggctcaac cttcttcgcg gatctgtgct tgcagataat tttgcaggcc
    10621 catcttctga atcagatcaa gttccgtttc cagccagtcg atatggcctt cttcatcacg
    10681 caaaatttct atcatcatat cgcggctgac gtaatcatga acgctatcgg cataaccaat
    10741 tgcctcacgc aaattcttcg cgccatccag ctcaagtgcc agatcagaac gcagcatttc
    10801 ctcaacatct tcaccaatgt tcagtttgcc caagtcctgt aagtttggaa gaccttccag
    10861 aaaaagaatg cgctcaatat aacgatcggc gtgtttcatc tcatcaatgg attcatgata
    10921 ctccacatca ttgagacgtt tgagacccca gtttttaaac attcgggcat ggagaaagta
    10981 ctgattgatt gcgacaagct catttcccaa cagtttgttg agataattta taactttagt
    11041 atcacctttc attttttgac tccttcgctt ccactctttg aagcgtagaa cggcttacga
    11101 gtaagtcaaa aaacacactc cgtttatgcg gactccttaa actccggcag ctgcattaat
    11161 tcatcctcca tcacttcacg cgcggcccgg acacatttac cgcactgatt gccgaccgga
    11221 ataaattttt ttaattgctg gaacgagtga ggggaaaact ggcgcacagc ctgacgaatt
    11281 tttttatcgc tgataccatt acaaagacaa acgtacatag agactcccgt atactttctt
    11341 caaaaataaa tgaaaatagt tcttatttca attacggaaa gactcttttt taagcaaaga
    11401 atagaaatat gaataaaaat aatgggctac caaaagtaac ccattgacaa aaaatgcggc
    11461 gataccggaa ggtatcgcca acacatttat tgcttagtaa acggcgcgaa gatttcgcta
    11521 aatgcgtact tcggtgcctg ttctttagtc aggccgtgga attccggaga caggttagtg
    11581 ccgcccggca ggtcgcgcgc gattgaccag ataccaacca taccgagatt acgcttctgc
    11641 gcatcctgca tgaccagacg agcatcagag agatagaaca cctcgccctg aacgtcgttc
    11701 acgccaacca tcggcgtggt acccatcata gcgtcaattt ctgcatcgct cttgttggga
    11761 tggaggcctt tcaattgtga atgcaggttg gcaatcgcag acgttgcaca cttaccgtga
    11821 atgttctggc cttcggtatt tgcagactga caaatcgcgt taccgtagtc cattgtcatc
    11881 acgttcacac ccgccagctc aacacctttc gctttggcat cgctcaggac attcatccct
    11941 tccggcgtca ggccagtcgg tagaattggc aaggtgtacc agatagcaat atctttgcct
    12001 tctgacttcc atttatcctg cactttcttc acagcaaggt tacgacgttc aatagatgcc
    12061 tgatccgcaa cccaggtgcc ttcgatatcg aagtccagga ctctgaggtt caggttatca
    12121 acgatgtcat aataatgctg catcagatcg tctacgttct tacaggaagc agccagcgga
    12181 gcgttgttag caccaccgat agacagcatc acatcgccgc cagcctcacg cagagctttg
    12241 attttgctgt actgagcgta attctgcata ccataagcgg taccccatgt cggcagacag
    12301 gtgttcgcat ctttactcac cacaaacgcc agcgtgaagt ggttgacgtt atgattctta
    12361 gccagcgcag ccaaatccgg gatcgtattc agcgtaaagt caacatacgg tgcataaacg
    12421 tgtttcggcc atgctttctt cggcgtacct acacgttctt tggttccggt ccagtcaaca
    12481 aaaacacgcc acgggttgct gtcagaagga gaaactttct gcactttact ctgagaaatg
    12541 tagttcacac cgttaaagcg aatcagcgta tcgctggcat aaagcgtttc tggattaaac
    12601 tgcggcgcat tattcagctc ttcgttgcta tagctctgag ccttacctaa cggcttccac
    12661 gggcggctat tgctaccggt ggcgttttgg ttcgcaacca gagcagggtt gtcactttgc
    12721 gtccagaaca gcgcttcata agcctgccca tcaacaacaa cgcgatcacc tttcacgtac
    12781 acagtggatg cggaccatgc tttcgccggt tcgtatttct tccacgggtt atcgccgccc
    12841 gggacaaagc cccatgcgtc ttgatcgaca gaagttttat aatgtgcatc gttataatct
    12901 acgatgctgt ccgcgctgta cgctttgctg gcctggaaac catccgcaac aatgacgccg
    12961 ccgttatcaa tttcacaggt agtcgggtta ccgtactgac tcaattccgc agcggtagct
    13021 gtacgctcga gacgccatgg gttattggag ttttcagcgc tggtaccccg tgggcaatct
    13081 ttagacccaa cccaccaggc gttcttgtaa acattaccat tgaaaataac atggtaaatt
    13141 tcgctacctt cctggccgct ccacgcttgc aatgaataat cgttaccgct atccgctggt
    13201 gttgatggct cgctgttatc cggagttggg gtcaccggtg tttccggagt tggatcaacc
    13261 ggagtcacag caccgccatt attatccggc ttaacggaac aggagccagg gttaccatac
    13321 tggctgattt ctgttgccgt tgccgcgcga acataacgcc atgggttact ggcatcgttt
    13381 tctttcgcat cgcctgggca atttgccgcg cctacccacc aggcgttctt ataaatgccg
    13441 ccgttataga taacgtacca tgtagaagaa ccttcctgac ctttccaggc tactacagca
    13501 tagttgtcgt ttgacttatc agctggggct ggctgaacat caggagccgg tttaacgtcc
    13561 ggtgccggtt tcacatccgg tgcaggctgt ggtgctgaag tacaggactg tggattactg
    13621 gtttctgaga tttccgttgc cgtagcggca cgaacataac gccatgggtt gctggcatca
    13681 ttgcctttcg catcacccgg acagttagaa gaggctaccc accaggcatt tttatatacc
    13741 gcaccattaa agacgacata ccaggtctga ccgccctgct gtttattcca ggcaactaca
    13801 gaagaattag acggaacagt gccctgtgct ggtgtagccg aaccgccatt agccggcgta
    13861 ttggatgcag gcgtatttga atttgaagaa gatgagctgc cgctcttttc gcaggaaagt
    13921 gtattgccaa actgactaat ttcagcagct gttgcggtac gctttaaacg ccacgggtta
    13981 gttgcatcat ttgctttcgc ttttcccggg caatttgtag aagaaaccca ccaggcattt
    14041 tcataaattt tgccatcgaa aataacctga tatttattac caccttgttg gttattccat
    14101 gcttccattg ccaatgctgg cagagc
//
LOCUS       DAPHBJ010000110        14043 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.111, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000110 DAPHBJ010000000
VERSION     DAPHBJ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.111"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(181..1092)
                     /gene="gsiD"
                     /locus_tag="RHZ76_002923"
     CDS             complement(181..1092)
                     /gene="gsiD"
                     /locus_tag="RHZ76_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HDV2813527.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAIFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1095..2015)
                     /gene="gsiC"
                     /locus_tag="RHZ76_002924"
     CDS             complement(1095..2015)
                     /gene="gsiC"
                     /locus_tag="RHZ76_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HDV2813528.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2033..3571)
                     /gene="gsiB"
                     /locus_tag="RHZ76_002925"
     CDS             complement(2033..3571)
                     /gene="gsiB"
                     /locus_tag="RHZ76_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HDV2813529.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLDQ
                     ALKTNDPVEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3591..5462)
                     /gene="gsiA"
                     /locus_tag="RHZ76_002926"
     CDS             complement(3591..5462)
                     /gene="gsiA"
                     /locus_tag="RHZ76_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HDV2813530.1"
                     /translation="MPHSDELDAGDVLAVENLKIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDSEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAGVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5449..6414)
                     /gene="iaaA"
                     /locus_tag="RHZ76_002927"
     CDS             complement(5449..6414)
                     /gene="iaaA"
                     /locus_tag="RHZ76_002927"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDV2813531.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDASVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6618..7853
                     /gene="moeA"
                     /locus_tag="RHZ76_002928"
     CDS             6618..7853
                     /gene="moeA"
                     /locus_tag="RHZ76_002928"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000397372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDV2813532.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEVCEAVVMQEQTEQTDNGVRFTVEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGR"
     gene            7853..8602
                     /gene="moeB"
                     /locus_tag="RHZ76_002929"
     CDS             7853..8602
                     /gene="moeB"
                     /locus_tag="RHZ76_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HDV2813533.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALARINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8678..9340)
                     /gene="fsa"
                     /locus_tag="RHZ76_002930"
     CDS             complement(8678..9340)
                     /gene="fsa"
                     /locus_tag="RHZ76_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDV2813534.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     ELHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GITTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            9471..10370
                     /locus_tag="RHZ76_002931"
     CDS             9471..10370
                     /locus_tag="RHZ76_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDV2813535.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10376..12808
                     /locus_tag="RHZ76_002932"
     CDS             10376..12808
                     /locus_tag="RHZ76_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDV2813536.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQMLEREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLIDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLAQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            12954..13769
                     /gene="ybiV"
                     /locus_tag="RHZ76_002933"
     CDS             12954..13769
                     /gene="ybiV"
                     /locus_tag="RHZ76_002933"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HDV2813537.1"
                     /translation="MSVKVIVTDMDGTFLNDVKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHVALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNKAPFTL"
     gene            complement(13798..13968)
                     /locus_tag="RHZ76_002934"
     CDS             complement(13798..13968)
                     /locus_tag="RHZ76_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813538.1"
                     /translation="MPCIDFPAFDLMSNIMQVGEKTFMKLRDPLDDEYYLQGPGTTLV
                     AELIEQDECNVH"
BASE COUNT         3385 a         3896 c         3579 g         3183 t
ORIGIN      
        1 catgttaata catttaacaa aataactgtc tgaacgctgt gccgctgcaa aaatagtccc
       61 ttgctgcggc tttttaacca aattacccga ttctacaaaa tcagcagaaa gttacaacac
      121 ttgcttaggg caataatttc ctgtgaggtg attacccttt caagcaatat tcaaacgtaa
      181 ctatccttta attttcggat ccagcgcatc gcgtaaacca tcgcccaaca aattgaacgc
      241 cagtacggtc agaaaaatag ccagggccgg aaaaatagcg acatgcggcg cgataaccat
      301 atccgctcgt gcctcattga gcattgctcc ccactctggt gtcggcggtt gagcaccgag
      361 gccgagaaat gacaggctgg cggcagagat aatcgaggtg ccaatgcgca tggtgaaaaa
      421 caccacgata gacgagacgg tcccaggcaa gatatgacgc aataaaatgg tcatatcgct
      481 ggcaccaata ctgcgtgctg actcaataaa ggtttgctgt ttcaacacca gcgtgttgcc
      541 gcgcaccagt cgggcaaacg cggggatgga aaaaatggcg actgcgataa tcacgttagc
      601 aatgccgctt cccaacaccg caacaacagc gatcgccagt aaaatacccg ggaaggcaaa
      661 cagcacatcg caaatgcgca tgatcagccg atcccaccag ccttcataat atccagccag
      721 caagcccagc aacgtcccga tcgccgcacc gataaacacg gcaaacacgc ccgccgccag
      781 cgagatttgc gcaccaacca ggacacggct gaaaatgtca cgccccagtg aatcgacgcc
      841 aaaccagtgc tgcaaagaag gtccgttatt cagattgtca taatcaaaat aattttcggc
      901 gtcataggga gcgatccagc gtgcaaaaat agccaccaca atcaataaaa taacgaacaa
      961 tgcggcggtc atcgccatat gctgacggcg aaatcgtcgc cagaattcat gccacggtgt
     1021 acgtacctgg tcaggtttga ccagtggcat ggcgtttaac accgcctgac gtcgccagtt
     1081 aaatagtcgc atccttactt gtacctgata gccgggttaa tggcggcgta aagcacatcc
     1141 accactaagt tgataagaat aaattccagc gagaaaagca gaatttccgc ctgaatcacc
     1201 gggtaatcgc gcatttctac ggagtcaacg agtaagcgtc caagtccggg ccagttgaaa
     1261 actttctcca ccacgataga accaccgagc aaaaagccaa actgtaagcc catcatggtc
     1321 actaccggga tcatcgcgtt gcgtagcccg tgtttgagga caacccaggt ttcgctcacc
     1381 cctttcgccc tcgcggtacg catataatct tcgcttaaaa cgtcgacaaa cgacgcgcgg
     1441 gtaaagcgcg ccatcacggc ggccactgcc gcgccgagcg tcagggaggg taaaatgtag
     1501 tgctgccaac tgtctgctcc cacggtaggc agccagccca gttcaacgga gaatacctga
     1561 attaaaagca tccccagagc aaatgccgga aacgagatcc ccgacaccgc aatggtcata
     1621 ctcaatcgat ccggccaacg attacgccag acggcggcga taattcccgc cgccatacca
     1681 aatatcaccg cccagaccat actggttatg gtcagccaca gcgttggcat aaagcggctg
     1741 gcaatctcat cggcaaccgg acgacgcgac accatcgaca ggccaaaatc cccctgcaca
     1801 gcattgctga tatagtgcca gaactggtga tacagcggct gatccaaccc cagctgctga
     1861 cgcaccagtt ctataacctg cgcatcagct tcgggcccgg caatcaatcg cgccggatcg
     1921 ccgggcagca tatgcacaaa taaaaacacc agcaccgaga cgataaacag cgttggaatc
     1981 aaccccagta agcgtttgat aacgtaatta agcattccac tccctgcgtt gcttattgca
     2041 aatccgcgtc ttcaaagctg aagccggtgt ctggcatgat ccaaaaaccg gtcaggtttt
     2101 tactgtgtgc cgacaccagt ttttctacca ccagcgggat ccacggcgat tcttgccaga
     2161 tgatatcctg cgccgcctta tataagcggg tcttttccac cggatcattc gttttcagtg
     2221 cctgatccag gaagtcatcc acctgtttat tgctgtaaaa cgcggtatta aacagcgtcg
     2281 gtggccagtt ttgcgatgca aacagcggcg atagtgccca gtcagcttcg ccggttgaag
     2341 ccgaccagcc agtgtagaac atccgcacgc cgctctcttt ttgcccttta ccttcaactt
     2401 ctgccgcccg ctgtccggca tccatcgcag tcacctgggc tttaatcccg acctgcgcta
     2461 actgctgctg ggtaaattgc agcactttct gcgcggtgct gtggttatgt gacgaccaca
     2521 gcgtggtact gaaaccgttg ggatatcccg cctctttcag taattcgcgc gctttcactg
     2581 gatcgtaagg ccacggttta taactttgcg cgtaggcgat actcggcggt accacaccag
     2641 tagctggcgt tgcatagccc gcgaaggcta ctttcaccag cgccgggcgg ttaatggcgt
     2701 aattcagcgc ctcacggacc ttcgggttat cgaacggttt ttgcgtcacg ttcatactga
     2761 tataacgctg cataattgac ggactggcca tcaactcgat atttttgttt ttctccagca
     2821 gtgcggcctg ctcgtaagga atggggaaag caaactgcgc ttcaccggtt tgcagcattg
     2881 ccgcgcgggt gttgttatcc gccaccggac gccaggttat gctgtccagt ttgggcaatc
     2941 ctggctgcca gtaacccgcg aattttttca ccttcacaaa atcggtctga ttccaggtat
     3001 ccagttcata cggtccggtt cccaccggat gaaaaccaat ctccttgcca tatttttcca
     3061 gcgctgccgg tgaaatcatc gcggtcgccg gatgggcaag aatattaata aacgctgaaa
     3121 acggctgttt gagagtaatc tttaccgttg tcggatcgat cgcctccgtt ttggcaatat
     3181 tcttatacag gttatagcgt ttaagatgat tcgccggatc gctggcccgg tccagattcg
     3241 cttttactgc cgctgcgttg aaatcggtgc catcctggaa tttaatgcct tcccgcaatt
     3301 tcacggtgta tgtaaggccg tcatcggaaa cggtataact ctccgccagc acgtttttca
     3361 gtttcatctc tttatccaga ccaaacagcc cctggtaaaa cgatttcgct acggcctgag
     3421 ataacgtgtc atttgcgtca tacggatcga gcgtggtgaa gttcgatcct accgccacca
     3481 ccacatcttt ggcagcgaat gcacaagatg ccatcaacgc tgtcgcaatg cccagcgcca
     3541 ctaacccact acggtgtaca gctcttgcca ttttattctc cgcctgaatg ttatctacgc
     3601 atgaatgcgt attctgattg tggttgtgcg acgtaatgcc ccggcccgac gcattgcaac
     3661 gagacgcctg ccacctcttc gccacgcaga tgaatattgc tgggaagatc gtccgacagc
     3721 agtacacgct gcggtcgttg tcgggacggt tcagcgaccg gaactgccgc cagtaattta
     3781 cgcgtataag gatgctgcgg gttttcgaag accgcgcgcc gtgggccaat ttcaacaatt
     3841 tgcccgagat acatcaccgc cacacgatga ctaatccgct ctaccacggc catatcgtgg
     3901 gagataaaca gatacgcaat gccgaaatca cgctggagat cgagcaacaa gttgataatc
     3961 tgcccgcgaa tagaaacatc cagcgccgaa acggcttcgt cggcaatgat cacttttgga
     4021 ttcaatgcca acgcgcgagc aatgcagatg cgctggcgct gaccgccgga aaactcatgc
     4081 gggtaacgcc aggcatgttc aggtaacagg cccacgcgct ccagcaacca cgcaacgcgt
     4141 gcagccgcgt ctttacctgg caataaaccg tgtacgcgca gcggttcgat aatcgaatca
     4201 ccgatggtct gacgtgggtc cagcgaagcg taagggtcct gaaaaataaa ctgaatatcc
     4261 cggcgtaatg cctgaagttt gccgggtgac aaggtatcga ttcgctgacc gttaaagata
     4321 atttcgccgc cctgcgattc gaccaggcgc agcaacgccc gcccggtagt ggatttaccg
     4381 ctgccagact cgcccaccag cgatagcgtt tcgccaggcc agagatcaaa actgactttc
     4441 tcaacggcat gcacttcccg cgttacgcga ttcaacaaac cgctgcgcaa agggaaacgg
     4501 gtgacaagat tacgcacccg taaaacaggt tcgctatcca ccaccgtttt ctgctcgatg
     4561 gggggttcct gtttcgctgg atgttcaagc gatatcaatg ggaaacgtcg gggataatct
     4621 aaccctttca tcgcaccaag ttgcggaaca gcagctaaca gcgcacgggt gtaaggatgt
     4681 tgcggtgcat gaaaaatctg ttcgacggta cccgtttcca ccgcctcgcc ctgatacatc
     4741 accagtaccc gatcggcaat ctctgccacc acgcccatat cgtgagtgat aaagataacg
     4801 cccatcgaca tctctttttg caatactttg attaattgca ggatctgcgc ctgaatagtg
     4861 acatccagcg cggtggttgg ctcatcggca atcagcaccg ccgggcggca tgacagcgcc
     4921 atcgcaatca tcactcgctg gcgcatcccg ccagagagtt gatgcggata acgtgaaaga
     4981 atggtttgtg cctccggaat gcgtacctga tccagcatcc gcttcgcctc gaccatcgct
     5041 tcttcacgac tggcgttctg atgcagacga attgattcgg caatctgttc acccacagta
     5101 aatactgggt tcagcgatgt catcggctcc tgaaatatca tcgccatatc cgcaccgcgc
     5161 acatggcgca tttgtgcagc gctctgctcg ctaagttcaa tcacttcacg actgcgccgc
     5221 cgcaacagca ttttatcgca ctgtactaaa ccgcccgcct gttccaacag acgcatcaat
     5281 gccagcgcag tcactgactt accggagccg gattcgccaa caatggccag cgtctcaccg
     5341 cgttgaaggc taaaagagag attgcggacc gcagctattt tctgctggtc ctgcataaag
     5401 gcaattttca gattttcaac cgccagcaca tcaccggcat caagttcatc actgtgtggc
     5461 aacggtgtcc cctttttcac ggtagatacc ggtggttggc gtatcgcctg cgtagcccca
     5521 ggcgcgatac attccttcgg tgttaaacgg tagcgcgaca ttcccttcat ggtcgatagc
     5581 gattaagcca ccgctaccgc caagcgcagg gagtttttcc attactaccc gctcgcaggc
     5641 ttccgcgaga cttaatccgc cgtaatccat taacgcggcg atgtcatatg ccgccagcgc
     5701 gcggatgaag acttcgcccg tgccggtaca agaaaccgcc acactggcgt tattggcgta
     5761 gcatccggca cccactaagg gactatcgcc aactcgtccg ggtaatttat tggtcattcc
     5821 gcctgtggac gtggctgccg ccagattgcc gtctaaatcc aacgccacgg cccccacggt
     5881 gcccattttc tgtttttcat ccagtggcgc accgctatgg tcgaggactg ttgccccttc
     5941 ctcgcgcgct gccagtagtt gttcataacg caaaggcgtg gagaaaatct ccggtgagac
     6001 gcgctccatg ccatgagcaa acgcaaaatt ttctgcccct tcgccaatca tcatcacatg
     6061 cgggctttgc tccatcacca gccgggcggc aagaaccgga ttacgcagat gactaacgcc
     6121 cgccaccgca ccggctttca gggtgttacc atccatcaca ctggcgtcca gttcatgggt
     6181 ttcatcacgc gtaaagacag cgccaattcc ggcgttaaac agtggacact cttccagcag
     6241 acgcaccgct tccgtcacca catccagcgc actttcgccc gcttccagca ttttctgccc
     6301 ggtttcaaca atggcagaca acgcctcaat gtagcgtaat tcctgttgca gactcatctg
     6361 cgcgcggcta attgcacctg cgccaccatg aattgcaatg actgctttgc ccatagtatt
     6421 cgtccattaa gggtggctcg ccacttttgc tataaatatc agaattttta tgaattactc
     6481 attcaatttt tgaatataga aagatggata tggcatgtaa aggcaggacc cagcgctacg
     6541 cccgaggtgg catacagtct gtggcgtttt tctgacataa taggcaaatt cgattttgcc
     6601 tccgcaggag tgttttcatg gaatttacca ccggattgat gtcgctcgac accgcgctta
     6661 atgagatgct ttctcgcgtc accccactga ccgcccagga aacgctgcca ctggtacagt
     6721 gttttggtcg tattctggcg agcgatgtcg tttcgccact ggatgtcccg gggtttgata
     6781 actccgctat ggacggctac gcggtgcgtt tagccgatat tgcctccggg caaccgctgc
     6841 ccgttgccgg gaaatcgttt gccggtcagc cgtatcatgg tgaatggcct gcgggcacct
     6901 gcattcgtat tatgaccggt gcgccggtgc cggaagtctg cgaagcggtg gtgatgcagg
     6961 agcagactga acaaacggac aatggcgtgc gttttactgt tgaagtgcgt agcgggcaaa
     7021 atattcgccg tcgcggtgaa gatatctctg cgggtgcggt tgttttcccg gcgggaactc
     7081 gcctgactac cgcagagctg ccagtaattg cttcactggg gattgccgaa gttccggtga
     7141 ttcgtaaagt gcgtgtagcg cttttttcta ccggtgatga actccagttg ccaggccagc
     7201 cgctgggcga cggccaaatc tacgatacca accgtctcgc cgtacacctg atgttagaac
     7261 agctgggatg cgaggtaatc aacttaggga ttatccgcga cgatccacat gccctgcgcg
     7321 ccgcatttat tgaagccgac agccaggcgg atgtggtgat cagttccggt ggtgtttcgg
     7381 ttggtgaggc ggattacacc aaaaccattc tcgaagaact gggcgaaatt gctttctgga
     7441 aactggcgat taaaccgggc aaaccgttcg cgtttggtaa actcagcaat agctggttct
     7501 gcggcctgcc gggcaacccg gtttcagcga cgctgacctt ctatcaactg gtacagcctt
     7561 tgctggcaaa actaagcggt aacaccgcca gcggcctgcc cgcgcgccag cgcgtacgca
     7621 cagcgtcccg cctgaaaaaa acgccaggac gtcttgattt ccagcgcggc gtgctgcaac
     7681 gcaacgccga tggcgaactg gaagtgacga ccaccggaca tcagggttca catatattca
     7741 gctcctttag cctcggcaac tgctttatcg tgctggaacg cgatcgcggc aatgtggacg
     7801 tgggcgaatg ggtggaagta gaaccgttta acgcgttgtt cggaggccgg taatggcgga
     7861 actcagcgat caggagatgc tgcgctacaa ccggcaaatc attctgcgcg gttttgattt
     7921 tgacggtcag gaggcactaa aagattctcg tgtgctggta gtcggcctcg gtggactcgg
     7981 ctgcgcagcc tcgcagtatc tggcaagcgc cggtgtcggc aacctgacgc tgctcgactt
     8041 cgacacggtt tcgctctcga atctgcaacg ccagacgctg cacagtgatg ctacggtcgg
     8101 gcaaccgaag gtggaatccg cccgtgacgc cctggcgcgg atcaacccac atatcgcgat
     8161 tacgccagtc aatgcactgc tggatgacgc agaacttgca gcattgattg ctgaacacga
     8221 tctggtgctc gactgtacgg ataacgttgc ggtacgtaat caactgaacg caggctgttt
     8281 tgccgcgaag gtgccgctgg tttccggcgc ggcaattcgt atggaaggtc aaatcacggt
     8341 cttcacttat caggacggcg agccttgcta tcgctgcctc agccgtttgt ttggcgagaa
     8401 cgcgttaacc tgcgtagaag caggcgtgat ggcaccgttg atcggcgtaa ttggttcgtt
     8461 gcaggcaatg gaagcgatca aactgctggc aggttatggc aaaccggcca gcgggaaaat
     8521 cgtcatgtac gatgcgatga cctgtcagtt tcgcgaaatg aaactgatgc gcaatccggg
     8581 gtgtgaggtg tgcgggcagt aattgccacc tccaggcctg ataagacgcg gcactccgcc
     8641 acgtcatcag caatttatga ggatgcagaa cgtccggtta aatcgacgtt ctgccaaacg
     8701 cccccagcca gtcctgctca aacttcgcca ccgcggcatc gacagccgga tagctaatca
     8761 tctgttgtgc cacatccagt ggcagagtaa ttgattcaca tcctgccagt aagcagtcca
     8821 gcgcctgacg cggggttttg aaactcgctg ccagcacttt cgcctgcggc gcatgcattt
     8881 tcaataactg gtgtaagtcg gtcacagtct gaatgccgct accgccctga gcatcaatac
     8941 ggttgacgta aggcgcaaca tattccgcac ctgccagcgc cgacagcagc ccttgtgccg
     9001 cgccatatac cgcggttccc agcgtcgtaa tcccttccgc ttttaacatc ttaatcgctg
     9061 ccagcccctc ggcggtcacc ggaactttca ccacgatatc cgcaataata gaacgcagct
     9121 taagcgcgtc attaaccatc ccttcggcag tggtagccat tacctgggca aacagacgcc
     9181 cctgaccgcc catcgcttca tgaagttccg gaagcacaac atccagcggt tttttacccg
     9241 cggcgataat gcttgggtta gtggtcacac ccgccagcgg aaaaatacgt gacagcgcct
     9301 tcaccgcaac aacgtctgaa gtatccagat acagttccat aaccatcctc aaaatgtact
     9361 ttttgtctga atgtagacta tcacgggaaa actctctgaa cagttgacgc acatcaatct
     9421 aactttcatt cgaaagtatt ttaatgtttg aacgaaacag agaggcaact atgattttca
     9481 atattcagcg ctactcgacc catgatggcc ccggtatccg cacggtcgta tttcttaaag
     9541 gctgttcgct gggctgccgc tggtgtcaga acccggaaag ccgcgcccgc acgcaggatc
     9601 tgctgtatga cgcacgactg tgtctggaag gctgcgagct gtgcgctaag gccgcgccgg
     9661 aagtgattga gcgcgcgctg aatggtttgc ttattcatcg ggaaaagtta accccggagc
     9721 atctgacggc gttaaccgac tgctgtccga cacaggcgtt aaccgtgtgt ggtgaagtga
     9781 aaagcgttga ggagatcatg gcgaccgttt tgcgcgataa accgttttac gatcgcagcg
     9841 gcggcggctt aacgctttcg ggtggtgaac cctttatgca gccggagatg gcgacggcgc
     9901 tactgcaagc cagccacgag gcaggcattc atactgcagt agaaacctgt ctgcatgtgc
     9961 cgtggaaata tatcgcccct tctctgccct atatcgatct gtttcttgcc gatttaaaac
    10021 acgttgccga cgcgccgttt aaacagtgga ccgacggtaa cgccgccaga gtgctggata
    10081 acctgaaaaa actcgccgca gcgggcaaaa aaatcattat ccgcgtgccg ctgattcagg
    10141 gctttaatgc cgacgaaacc tctgtaaaag ccattaccga ttttgccgcc gacgagctgc
    10201 acgttggcga aattcatttc ctgccctacc acacgctggg catcaacaaa tatcacttac
    10261 ttaatctgcc ctatgacgcc ccggaaaaac cgcttgatgc gccagaactg ctcgattttg
    10321 cccagcagta tgcctgccag aaagggttaa ccgcgacctt acgaggataa caaccatgac
    10381 cacactgaaa ctggacacgc tcagcgaccg cattaaagcg cacaaaaatg cgctggtgca
    10441 tattgtgaaa ccgccagtct gtaccgagcg cgcgcagcac tataccgaga tgtatcaaca
    10501 acatctcgat aagccgatcc cggtacgtcg cgcgctggca ctggcgcatc acctggcgaa
    10561 tcgcactatc tggatcaagc acgatgagtt gatcattggc aaccaggcaa gcgaagttcg
    10621 cgccgcgccg atcttcccgg aatataccgt ctcctggatc gaaaaagaga ttgatgatct
    10681 ggcagatcgt ccgggtgctg gttttgcggt gagcgaagag aacaaacgtg ttctgcatga
    10741 agtgtgccct tggtggcgcg gtcagacggt acaggatcgt tgctacggca tgtttaccga
    10801 tgagcaaaaa ggtctgctgg cgaccggaat cattaaagcg gagggcaata tgacttccgg
    10861 cgatgcgcac ctggcggtca atttcccgct gctgctggaa aaagggcttg atggtctgcg
    10921 cgagaaagtg gcggaacgtc gctcgcgcat caacctgacg gtgctggaag atttgcacgg
    10981 cgagcagttc ctgaaagcga ttgatatcgt gctggtggca gtcagtgaac acattgaacg
    11041 tttcgctgcc ctggcgcgtg aaatggccgc gaccgaaacc cgcgaaagcc gtcgcgatga
    11101 actgctggcg atagcagaaa actgcgatct tatcgcccac cagccgccgc agacgttctg
    11161 gcaggcgctg caactgtgct acttcatcca gttgattttg cagattgaat ctaacggtca
    11221 ctcggtgtcg tttggtcgta tggaccagta tctctacccg tattatcgcc gtgatgttga
    11281 actgaaccaa atgctggaac gcgaacacgc catcgaattg ctgcatagct gctggttgaa
    11341 attgctggaa gtgaacaaga tccgctccgg ctcacactca aaagcctctg cgggtagccc
    11401 gctgtatcag aacgtcacca ttggcgggca aaatttgatt gatggtcagc cgatggatgc
    11461 ggtgaatccg ctctcctacg cgatcctcga atcctgcggt cgcctgcgtt cgacgcagcc
    11521 taacctcagc gtgcgctacc atgcgggaat gagtaacgat ttcctcgacg cctgcgtaca
    11581 ggtgatccgg tgcggcttcg ggatgccggc cttcaacaac gacgaaatcg tgatcccgga
    11641 atttattaaa ctcggtattg aaccgcagga cgcttacgac tacgcagcga ttggttgtat
    11701 cgaaaccgcc gtcggtggca aatggggcta tcgctgtacc ggcatgagct ttatcaactt
    11761 cgcccgcgtg atgctggctg cgctggaagg cggtcgtgat gccaccagcg gcaaagtgtt
    11821 cctggcacaa gaaaaagcgt tgtcggcagg taacttcaac aacttcgatg aagtaatgga
    11881 cgcgtgggat acgcaaatcc gttactacac ccgcaaatca atcgaaatcg aatatgtcgt
    11941 cgacaccatg ctggaagaga acgtgcacga tattctctgc tcggcgctgg tggatgactg
    12001 tattgagcga gcaaaaagta tcaagcaagg cggcgcgaaa tatgactggg tttctggcct
    12061 gcaggtcggc attgccaacc tcggcaacag cctggcggca gtgaagaaac tggtgtttga
    12121 acagggcgcg attggtcagc aacagctggc tgccgcactg gcggatgact tcgacggcct
    12181 gactcatgag caactgcgtc agcggctgat taacggtgcg ccgaagtacg gtaacgacga
    12241 tgatactgtc gatacgctgc tggctcgcgc ttatcagacc tatatcgacg aactgaaaca
    12301 gtaccataat ccgcgctacg gtcgtggtcc ggttggcggc aactattacg cgggtacgtc
    12361 gtctatctcc gctaacgtac cgtttggcgc gcagacgatg gcaacgccgg acggacgtaa
    12421 agcgcatacc ccgctggcag aaggcgcaag cccggcttcc ggtactgacc atctcggccc
    12481 aacggcagtc attggatcgg tgggtaaact gcctacagca gcgattctcg gcggcgtgtt
    12541 gctcaaccag aaactgaatc cggcgacgct ggagaacgaa tctgacaagc agaaactgat
    12601 gatcctgctc cgtaccttct tcgaggtgca taaaggctgg catattcagt acaacatcgt
    12661 ttcccgcgaa acgctgctgg aggcgaaaaa acatcccgat cagtatcgcg atctggtagt
    12721 gcgtgtcgcg ggctattccg ccttcttcac cgcgctctct ccagacgctc aggacgatat
    12781 catcgcccgt actgaacata tgctgtaatc tcctcggccc ggcggcgtcg tcgccgggct
    12841 aaatcattca catcatcaat ttcatcctta ctttcattcg aaatataatt tgtgctctac
    12901 gtcacattgt tattacactg ttacggtttc attttcgagc cgggagcaca cctatgagcg
    12961 taaaagttat cgtcacagat atggacggta cttttcttaa cgacgtcaaa acgtacaacc
    13021 aaccacgttt tatggcgcaa tatcaggaac tgaaaaagcg cggcattgag ttcgttgtcg
    13081 ccagcggtaa tcagtattac cagcttattt cattctttcc tgagctaaag gatgagatct
    13141 cttttgtcgc ggaaaacggc gcactggttt acgaacatgg caagcaactg ttccacggcg
    13201 aactgacccg acatgaatcg cggattgtta ttggcgagtt gctaaaagat aagcaactca
    13261 attttgtcgc ctgcggtctg caaagtgcat atgtcagcga aaacgccccc gaagcattcg
    13321 tcgcactgat ggcaaaacac taccatcgcc tgaaacctgt aaaagattat caggagattg
    13381 acgacgtact gttcaagttt tcgctcaacc tgccggatga acaaatcccg ttagtgatcg
    13441 acaaactgca cgtagcgctc gatggcatta tgaaacccgt caccagtggt tttggcttta
    13501 tcgacctgat tattcccggt ctacataaag caaacggtat ttcgcggtta ctgaaacgct
    13561 gggatctgtc accgcaaaat gtggtagcga ttggcgacag cggtaacgat gcggagatgc
    13621 tgaaaatggc gcgttattcc tttgcgatgg gcaatgctgc ggaaaacatt aaacaaatcg
    13681 cccgttacgc taccgatgat aataatcatg aaggcgcgct gaatgtgatt caggctgtgc
    13741 tggataataa agcgccgttt actctttaac gaaagtcggg gcaattgccc cgatttctca
    13801 atgaacatta cactcatctt gttcaatcag ttctgccact aatgtggttc caggcccctg
    13861 taagtaatat tcatcgtcaa ggggatcgcg cagtttcata aacgtttttt caccaacttg
    13921 cataatgtta ctcatcagat cgaaggctgg gaagtcaata cagggcatgc taacatctcc
    13981 acacctttag aaagggatac gataggtttt gatctaacat tatttgaggg ttatcaaaat
    14041 gca
//
LOCUS       DAPHBJ010000111        13841 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.112, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000111 DAPHBJ010000000
VERSION     DAPHBJ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.112"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..345
                     /gene="ecpR"
                     /locus_tag="RHZ76_002935"
                     /gene_synonym="matA"
     CDS             <1..345
                     /gene="ecpR"
                     /locus_tag="RHZ76_002935"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HDV2813539.1"
                     /translation="SAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAGLSRDIRKE
                     LAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTVYTHRRNAE
                     AKLYSKIYKLVQ"
     gene            371..1006
                     /gene="ecpA"
                     /locus_tag="RHZ76_002936"
                     /gene_synonym="matB"
     CDS             371..1006
                     /gene="ecpA"
                     /locus_tag="RHZ76_002936"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HDV2813540.1"
                     /translation="MTHWDITSSIQLGKKCNEKKVLAIALVTVFTGMGVAQAADVTAQ
                     AVATWSATAKKDTTSKLVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKL
                     TSRLITNTLTQLDTSGSTLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYN
                     ASNRTTAQDGFTFTIISGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1064..1732
                     /gene="ecpB"
                     /locus_tag="RHZ76_002937"
                     /gene_synonym="yagY"
     CDS             1064..1732
                     /gene="ecpB"
                     /locus_tag="RHZ76_002937"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HDV2813541.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            1758..4283
                     /gene="ecpC"
                     /locus_tag="RHZ76_002938"
                     /gene_synonym="yagX"
     CDS             1758..4283
                     /gene="ecpC"
                     /locus_tag="RHZ76_002938"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HDV2813542.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSRLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4273..5916
                     /gene="ecpD"
                     /locus_tag="RHZ76_002939"
     CDS             4273..5916
                     /gene="ecpD"
                     /locus_tag="RHZ76_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HDV2813543.1"
                     /translation="MRVNLLITMIIFALIWPVTALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYMQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            5885..6595
                     /gene="ecpE"
                     /locus_tag="RHZ76_002940"
     CDS             5885..6595
                     /gene="ecpE"
                     /locus_tag="RHZ76_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HDV2813544.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPQDNRERYYRVSFREVPTRNQTRRSPTGGEVSTDPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            6908..7237
                     /locus_tag="RHZ76_002941"
     CDS             6908..7237
                     /locus_tag="RHZ76_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDV2813545.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(7485..8099)
                     /locus_tag="RHZ76_002942"
     CDS             complement(7485..8099)
                     /locus_tag="RHZ76_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HDV2813546.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENLSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            8517..9206
                     /gene="paoA"
                     /locus_tag="RHZ76_002943"
     CDS             8517..9206
                     /gene="paoA"
                     /locus_tag="RHZ76_002943"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit"
                     /protein_id="HDV2813547.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDTLRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAALKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            9203..10159
                     /gene="paoB"
                     /locus_tag="RHZ76_002944"
     CDS             9203..10159
                     /gene="paoB"
                     /locus_tag="RHZ76_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HDV2813548.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIHPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFSSAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            10156..12354
                     /gene="paoC"
                     /locus_tag="RHZ76_002945"
     CDS             10156..12354
                     /gene="paoC"
                     /locus_tag="RHZ76_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HDV2813549.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEGTPDK
                     NVGDFDGAFSSAAVKIDATYTTPDQSHMAMGPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPTIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPADP
                     TRRFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEPNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACVKLREMIASAVGFDPEQSQFADGKITNGTRSAMLHEATAGGRL
                     IAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            12364..13320
                     /gene="paoD"
                     /locus_tag="RHZ76_002946"
     CDS             12364..13320
                     /gene="paoD"
                     /locus_tag="RHZ76_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HDV2813550.1"
                     /translation="MSYSLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTK
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETTQ
                     IRAPVGIFPKARDAHTLALSVLAEVASVRLHQEEDSCLPPSS"
     gene            13371..13709
                     /locus_tag="RHZ76_002947"
                     /pseudo
     CDS             13371..13709
                     /locus_tag="RHZ76_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308332.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         3402 a         3720 c         3724 g         2995 t
ORIGIN      
        1 tccgcggtaa aggggaaaca ggtcgtattg attgcggcca gaaagtcaga agccttagca
       61 aattattggt attacaatag caatattagg ggcgtggtat acgctggact gagtcgtgat
      121 attagaaaag aactggccta tgtgattaat ggcaggttcc tgagaaaaga tattaagaaa
      181 gataaaatca cggaccggga aatggaaatt atccgcatga cggcccaggg aatgcaacct
      241 aaatcgattg ccagaattga aaattgtagt gtgaagacag tgtataccca tcggcgtaat
      301 gctgaggcca agctgtactc aaaaatatat aagttggttc agtaaactcc aggcaagtta
      361 gttttaaaaa atgactcact gggacatcac gtcctcaatt caactcggga agaaatgcaa
      421 tgaaaaaaag gttctggcaa tagctctggt aacggtgttt accggcatgg gtgtggcgca
      481 ggctgctgac gtaacagctc aggctgtagc gacctggtcg gcaacagcca aaaaagacac
      541 caccagtaag ctggttgtga cgccactcgg tagcctggcg ttccagtatg ccgaaggcat
      601 taaaggtttt aactcacaga aaggtctatt tgacgtggct atcgagggtg actcaacggc
      661 taccgccttt aaactgacct cacgtcttat caccaacaca ttaacccagt tggatacctc
      721 aggttccaca ctgaatgtgg gcgtggatta taacggcgcg gcagtcgaaa aaactggcga
      781 taccgtgatg atcgataccg ccaacggcgt actgggcggc aaccttagcc cgctggctaa
      841 cggttacaat gccagcaatc gtaccaccgc acaggatggt ttcaccttca ccatcatcag
      901 cggcaccacc aatggtacca ccgcagtaac cgattacagc actctaccgg aaggcatctg
      961 gagcggcgac gttagcgtac agttcgacgc gacctggacc agttaatctc tctgatgtac
     1021 cagcaggggt agcccccctg ctttattcct ggacggaatt gttatgaaaa agcaccttct
     1081 gcctctcgct ctgctgtttt ccggaatatc tccggcccag gcgctggatg tcggcgatat
     1141 atcatcgttt atgaacagtg acagcagcac gctgagcaaa acgatcaaaa acagtaccga
     1201 cagtggtcgc cttatcaata tccgtctcga acggctctct tcaccgcttg acgacgggca
     1261 ggttatctca atggacaagc cggatgagtt gctactcact cccgccagct tgctgctacc
     1321 cgcccaagcc agcgaagtga tccgcttctt ctataaggga cccgcagatg aaaaagagcg
     1381 ctactaccgc attgtctggt ttgatcaggc cctcagtgat gcacagcgcg ataatgccaa
     1441 ccgcagcgct gtggccactg cttccgcccg catcggcacc attctggtcg tcgcaccccg
     1501 ccaggcaaac taccactttc agtacgccaa cggctccctg acaaatacag gaaatgcgac
     1561 gctgcggatc ctcgcctacg gcccctgcct gaaagccgcc aatggtaaag agtgtaaaga
     1621 gaattactac ctgatgccgg gcaagtcgcg tcgttttacc cgcgtggaca cggcggataa
     1681 caaaggacgg gttgcacttt ggcagggtga taagttcatt cccgtgaaat agatagctgt
     1741 gcagatggat aacgacaatg cctttacgac ggttctcccc aggactgaaa gcccagtttg
     1801 ccttcggcat ggtctttttg ttcgttcagc ccgatgccag cgctgctgac ataagtgcgc
     1861 agcaaatagg tggggtgatt attccgcagg ccttcagtca ggcgcttcag gacggcatga
     1921 gcgtcccgct ctatattcat ctcgccggta gccagggtcg ccaggacgat cagcgaatcg
     1981 gcagcgcttt tatctggctg gacgatggac agctacgcat ccggaaaata cagctggaag
     2041 agagtgaaga taacgccagt gtcagcgaac aaactcgaca gcagctgatg gctctggcca
     2101 acgccccgtt caatgaggcc cttaccatcc ccctgactga caacgcgcag ctggatctca
     2161 gcttgcgcca actgcttctg cagctagtgg tcaagcgcga agcgctgggc accgtactac
     2221 gctcacgtag cgaagacatc gggcagtcca gtgttaacac cctcagcagt aatctgagct
     2281 ataacttggg cgtctataac aaccagttgc gtaacggcgg gagcaacaca tccagctatc
     2341 tgtcgctgaa taacgttact gcactgcgcg aacatcatgt ggtgctcgac ggctcgctgt
     2401 acgggatcgg tagcggtcaa caggacagtg aattatataa agcgatgtat gaacgcgatt
     2461 ttgccggtca ccgatttgcc ggtggaatgc tcgacacctg gaacttgcag tccttagggc
     2521 cgatgaccgc catttcagca gggaagattt acggcctttc ctggggaaac caggccagct
     2581 ccaccatctt cgacagcagc cagtcagcca cgccagtgat cgccttttta ccggcggcgg
     2641 gcgaagtaca tctcacccgt gatgggcggc tactaagcgt tcagaacttc actatgggca
     2701 atcatgaagt ggatacccgg ggtctaccgt acgggattta cgatgtggaa gttgaggtga
     2761 tcgttaacgg tcgcgtgatc agcaaacgca cccagcgggt caataagctg tttagccggg
     2821 ggcgcggcgt cggtgcacca ctggcgtggc aggtatgggg cggtagcttt catatggatc
     2881 gctggtcgga aaacgggaaa aagacgcgac cagctaaaga gagttggcta gcaggtgcct
     2941 cgacctccgg ctcattgagt acgcttagct gggcggcaac gggatatgga tacgataatc
     3001 aggcggtggg tgaaacccgt ctgacgctgc cgcttggggg ggcgatcaac gttaacctgc
     3061 aaaatatgct ggccagtgac agctcatgga gcagcatcgg cagcatcagc gccactctac
     3121 cgggaggctt tagttcgctg tgggttaatc aggaaaaaac ccgcattggc aatcaattgc
     3181 gacgtagcga tgccgacaac cgtgcaatcg gcggcacact caacctgaac tcactgtggt
     3241 cgaagctggg cacattcagc atcagctaca atgatgaccg ccgttacaac agccattatt
     3301 acacggcaga ttactatcaa aatgtctaca gcggtacctt tggttcgctt ggcctgcggg
     3361 ccggtattca gcgctataac aacggcgaca gcaacgccaa tacaggaaaa tatatcgctc
     3421 tcgatctctc gctaccactg ggcaactggt ttagcgcagg gatgactcat caaaacggct
     3481 acaccatggc aaacctgtca gcacgcaaac agtttgatga aggaaccatt cgcactgttg
     3541 gtgccaatct gtcacgtgcc atctccggcg ataccggtga tgacaaaacc ctcagcggcg
     3601 gggcgtatgc acaattcgac gctcgttacg ccagcggaac gctgaacgtc aatagcgcgg
     3661 cggacggcta cgtcaatacc aacttgaccg ccaatggcag cgtcggctgg cagggtaaaa
     3721 acattgctgc cagcgggcgg actgatggca acgctggggt gatattcaac accgggctgg
     3781 aggacgacgg tcagatcagc gccaaaatca acgggcggat tttcccgctt aacggcaagc
     3841 gtaactatct cccgctctct ccctatggaa gatatgaggt ggagttacag aacagcaaaa
     3901 actcactcga cagttacgat atcgtcagcg gccgcaaaag tcgtctgact ctctatccag
     3961 gcaatgtcgc tgtcattgag ccagaggtga agcagatggt taccgtctcc ggtcgtatcc
     4021 gtgcggaaga cggcacactg ctggctaacg cacggattaa caaccatatc ggccgaaccc
     4081 gaaccgatga aaacggcgag tttgtcatgg acgtggataa gaaatacccc actatcgatt
     4141 ttcgctacag tggcaataaa acctgcgaag tggctctgga actcaaccag gcgcgcggtg
     4201 ccgtctgggt cggtgatgtg gtctgcagcg gcctctcatc gtgggcggcg gtgacgcaga
     4261 caggagaaga gaatgagagt taacctacta ataacgatga taattttcgc gctaatctgg
     4321 ccagtaactg cgctcagagc ggcagtgagc aaaacaacct gggcggatgc accggcacgc
     4381 gagtttgtgt ttgtcgaaaa caactcagac gacaactttt tcgtcactcc tggcggggcg
     4441 ctggatccgc gcctgaccgg tgccaaccgc tggaccggtt taaaatacac tggttcagga
     4501 accatctatc agcaaagcct cggctacatt gataacggtt acaacaccgg cctttatacc
     4561 aactggaagt ttgatatgtg gctggaaaat tcaccagttt catctccttt aactggcttg
     4621 cgctgcatca actggtacgc tgggtgtaat atgaccacca gtcttatcct gccgcaaacc
     4681 accgacgcca gtggatttta tggcgcgacg gtgaccagcg gcggcgcgaa gtggatgcac
     4741 ggcatgttgt cagacgcgtt ttaccagtat atgcaacaaa tgcccgtcgg cagcagcttt
     4801 acaatgacca tcaatgcctg ccagacctct gtgaactatg acgccagcag cggcgcacgc
     4861 tgtaaggatc aggcctccgg caactggtat gttcgcaacg tcacccatac gaaagcagca
     4921 aatctacggt tgataaatac ccactcgctg gcggaagtat ttatcaacag cgacggagta
     4981 ccgactctgg gcgaagggaa cgccgactgc cggacgcaaa ccatcggcag ccgttcagga
     5041 ttaagttgta agatggttaa ctataccctg caaacaaacg gactcagcaa cacctcaatc
     5101 catatattcc cggcgatcgc caactcgtcg ttagcctcgg ccgtcggggc gtacgatatg
     5161 cagttcagtc tgaatggcag ttcatggaaa ccggtgagca ataccgccta ttactacacc
     5221 ttcaacgaga tgaagagcgc agactcgatc tatgttttct tctcgagcaa cttctttaag
     5281 cagatggtga acctcgggat cagcgatatc aacaccaaag atctattcaa ctttcgcttt
     5341 cagaacacca catcaccgga gtctggctgg tatgaatttt ctacctccaa cacgctgatt
     5401 atcaaacccc gtgatttcag catcagtatt atctccgatg aatatactca gacaccgtcg
     5461 cgggagggat atgttggcag cggcgagtcg gcactcgatt tcggctatat cgtaaccacc
     5521 agcggtaaaa cagctgccga cgaagtgctg atcaaggtga ccggacccgc gcaggtgatt
     5581 ggcgggcgct cctattgtgt cttcagctcc gatgacggta aggcgaaagt accgttcccg
     5641 gcgacacttt cctttattac ccgcaacgga gctacaaaaa cctacgatgc cgggtgcgat
     5701 gatagctggc gggatatgac cgatgcgctg tggttgacca caccgtggac tgatatctct
     5761 ggcgaagtgg ggcagatgga taagaccaca gtcaaatttt cgattccaat ggataacgcc
     5821 atttcgctgc gtacggtaga tgataacggc tggtttggcg aagtcagcgc ttcaggagag
     5881 attcatgttc aggcgacgtg gcgtaacatt aactaaggcc ctgctgacag cggtctgtat
     5941 gctggcggca cctttgacac aggcgatttc ggtcggcaat ctgacatttt cgctgccgtc
     6001 cgagactgac tttgtcagca aacgtgtagt gaataacaac aaaagcgcac ggatataccg
     6061 tattgccatc agtgctattg atagcccggg cagcagtgaa ttgcgcaccc gaccggtgga
     6121 tggtgaactg cttttcgccc cccgccagct ggcgttgcag gctggtgaga gcgagtattt
     6181 taaattttac tatcatggtc cacaggataa ccgcgagcgc tactaccggg tctcatttcg
     6241 cgaggtcccc actcgtaacc agacaagacg cagcccaacc ggcggcgagg tcagcacgga
     6301 tccggtggtg gtgatggata ccattctggt agtacgacca cgtcaggttc agtttaaatg
     6361 gtccttcgac aaggtgacag gaacggtaag taacactggc aacacatggt ttaagctact
     6421 gattaagcca ggatgtgatt cgaccgaaga ggaaggcgat gcctggtatc tacgtccggg
     6481 agacgtggtt catcaacctg agttacgtca gccggggaat cattatctgg tctataacga
     6541 caaattcatt aagattagcg attcctgtcc ggctaagccc ccttcggcgg actaagactt
     6601 atccatcggt cgagggaaga attccacctc agagccccaa aaatcgttta aatgatgtgg
     6661 aagcgatcgt caatatcacc tgtaacgatg ggctttctca tatgcacacc atagtgttca
     6721 ggagacacgg aaaggtatca ggcagcctgc taacgagtgg tttaatcacg aattagtacg
     6781 taaaatcggt aacggctgga aatcattcaa tactcacact atcgaaagtt ccccagccaa
     6841 ccgcggtacg ttcttacata cgatgtaccg ctgttctctt tacgattttt agctgtactg
     6901 gtgaattatg agcaatctga atccatgcat gacgtgtggt gcctgttgtg catttttccg
     6961 cgtctctttt tactgggccg aagccgacga tgctggcggc actattcccg ccaggctcac
     7021 tgaacaaata tccccttttc accgatgcat gagcggtacc aatcagaaaa acccccgatg
     7081 tattgccctt gcaggaaccc cgggcaaaaa tgcctgctgc acgatatata aaaatcgatc
     7141 gtccacatgc agagaattcg ccatgtctgg tgagaacgga gtcgttaatg aggcttgcaa
     7201 tcgtgcaagg gctaaatacg ggctgacacc gttataaaca tacaataatt aattgcactg
     7261 ccccgccagc gataatagcg gggcttcgtt tttcaggggt aacaaaaccc gatacttctt
     7321 ctatttgcca gcaacaatgc ctctcttctg tagcgttctt cggatctaca tcatcctgaa
     7381 gtagcgcgat atactcactg actatctgaa tcagaatata aaaagcaatg tttttaacct
     7441 ataaaaatgg cgctgtattt gcgccatttt tatcattcaa tgcattatct gtttgagcct
     7501 aaagggatct cagggtctgg ctcatgagta attctgtttc gtaaatctct gcgaataatt
     7561 tcaatagacc agaaccagac taaatgtcca aaaatttcag aaagattctc ataccacggg
     7621 agatcaaaca gaggtggcgt cagtcccatg agagggaatg aaatcatatg aacaaaaagt
     7681 tgggctaaag cacctgccag taagccctgc cagagtttaa tttttggaaa tacttcagcg
     7741 accacacaat aaccgacagc aaacactatc gaaaagataa tgtgcgtaac accaacccag
     7801 ttaaagacat gcccggcaaa ggtataaaca gccgcattgg gatctgtcag ccccaaccag
     7861 tctcgaagaa aaatatacgg tggattgaga aaattacgcg agcaatcaat ttggcctgca
     7921 gccctgatta atgattccgg gccacacgct gcattaaaca tatccaccgg gctacgtggc
     7981 ggcaatggaa cttcagcccc ccacttcacg aatgcggaaa caacgccagc aatcagccca
     8041 atgaatgcag caagaccata acgtctgcgg ttcggtggag tttgttcaaa tatattcata
     8101 tctaccctgc ttgtaccatt atgttataca cctcttcagg agtattcata aaacaaggca
     8161 aatgtaaaga actgtattgt tttgtataac aagatagttt cctaatcgcc aatgaatata
     8221 agctccatca tttctgccta tttttatatt agaagtgaca gagatttgca gggtgatgca
     8281 gagctgaaaa tcacaggttt ccttattggt ttttgcatcg tacaactaaa gcaataaacc
     8341 agctcccatc atcatcaaca tcccggcgaa catgaattta ttagccgggc tcatagctgc
     8401 attcgcgttt taagaataat cctcctgctg tcgccgacta tgcttaacgt ttaaaaaagc
     8461 atcagcactc tcgcaacgca ctcttatttt cccctttaga ataccggagg cctggtatga
     8521 gcaaccaagg cgaatacccc gaagataatc gggttgggaa gcacgagccg cacgatttaa
     8581 gtttgacccg tcgcgatctg attaaagtga gcgccgcaac agcggcgacc gccgtggttt
     8641 atcctcattc tacgctggcg gcaagcgttc cggcagctac acccgcgcca gagataatgc
     8701 ccctgacact gaaggtgaac ggcaaaaccg agcagcttga ggtggatacc cgaaccacgc
     8761 tactggacac tttgcgtgaa aatctgcatt tgatcggtac caagaaaggt tgcgatcacg
     8821 gacagtgcgg agcctgtacc gtgctggtca atggtcgcag gcttaatgcc tgcctgacgc
     8881 ttgcagtcat gcatcagggg gccgagatca ccaccattga aggcctgggt tcgccagata
     8941 atcttcaccc catgcaggcg gcctttatca agcatgatgg cttccagtgc ggctactgca
     9001 cctccgggca aatttgctca tcagtagcgg cgctaaaaga gattcaggac ggcattccaa
     9061 gtcacgtcac ggtcgatttg gtttccgctc cagaaacaac tgccgatgag atccgtgaac
     9121 gtatgagcgg caacatctgt cgctgtggtg catacgccaa catccttgcc gccattgaag
     9181 atgctgcggg ggagataaaa tcatgaaggc gtttacctat gaacgagtga acaccccagc
     9241 cgaggcggca cttagcgctc agcgcgtacc cggcgcaaaa tttatcgcgg gcgggaccaa
     9301 tctgctggac ctgatgaagc tggaaattga aacgcccacc caccttatcg atgtgaacgg
     9361 cctcgggctc gataagattg aagtgaccga cgcgggtggg ctgcgcatcg gcgcactggt
     9421 acggaatacc gacctggcgg ctcacgagcg cgtgcgtcgt gattacgcgg tactctcccg
     9481 cgccctgctc gctggcgcat ctggtcagtt acgtaatcag gcaaccaccg caggtaatct
     9541 gctccagcgc acgcgctgcc cctattttta cgacaccaat cagccctgca ataagcgcct
     9601 gcccgggagc ggctgcgcgg cgcttgaagg ctttagccgt cagcacgcgg tggtaggcgt
     9661 aagcgaagcc tgcattgcca cccatccgag cgatatggcg gtcgcaatgc ggttgctgga
     9721 tgcggtggtg gaaaccatca cgccggaggg aaagactcgc agtatcacac tggctgattt
     9781 ttatcaccct ccgggaaaaa cgccgcacat tgaaaccgcc ctgcttcccg gtgagcttat
     9841 cgttgcggtg acgttacctc cgccgctcgg cggaaaacat atctaccgta aggtgcgcga
     9901 tcgcgcctcc tacgcctttg ccctggtatc ggtcgcggcg attattcacc ctgacggcag
     9961 cgggcgcgtc gcgctgggtg gagtagcaca taagccctgg cgcattgagg ctgcggatgc
    10021 tcagctatcc cagggggcgc aggccgtata tgacgcgctg ttctccagcg cccatcccac
    10081 cgctgaaaac acctttaaac tcctgttggc gaagcgaacg cttgcctccg tactggctga
    10141 agcgagggca caggcatgaa atttgataaa cccgcagggg aaaacccgat cgatcagctg
    10201 aaggttgtag gtcgtcccca tgaccgcatc gacggaccgc ttaaaactac cggcacggca
    10261 cgctacgcct acgaatggca tgaagaagcc cccaacgccg cctatggcta tatcgtcggt
    10321 tccgccattg ccaaaggacg cctcaccgcc cttgatacgg acgccgcgca aaaagcgccg
    10381 ggcgtactgg ctgtcattac cgccagtaac gccggggcac tcggcaaagg cgacaaaaac
    10441 accgccaggc tgttaggcgg ccccactatt gagcactatc atcaggccat tgcgctggta
    10501 gtggccgaga ccttcgaaca ggcgcgagcg gcggcctcgc tggtgcaggc gcactatcgc
    10561 cgtaataaag gagcttactc cctggcggac gaaaaacagg ccgtcaatca gccgccggaa
    10621 ggcacgcccg acaaaaacgt cggtgacttt gacggggctt tctcctccgc tgcggtgaag
    10681 attgatgcta cctacacgac cccggaccag agccatatgg cgatggggcc gcatgcctcg
    10741 atggccgtct gggatggaaa taagcttact ctctggacct caaatcagat gattgactgg
    10801 tgccgcaccg atctggcaaa aacgctgaaa gtgcccgtgg agaatgtgcg tattatctcc
    10861 ccgtatatcg gcggagggtt tggcggcaag ctgttcctga gaagcgatgc gctgctggca
    10921 gccctcgccg cccgagcggt gaaacgtccg gttaaagtga tgctcccccg ccccactatt
    10981 cccaataaca ccacgcaccg ccccgcaacc cttcagcacc tgcgtattgg tgctgaccag
    11041 agcgggaaaa tcaccgctat ctcacatgaa agctggtccg gaaacctgcc cggcggcacg
    11101 ccggaaacgg cggtacagca aagcgaatta ctctacgccg gagcaaaccg tcataccggc
    11161 ctgcggctcg ccacgcttga tttgccggaa gggaacgcca tgcgtgcgcc cggcgaagcc
    11221 cccggtctga tggcgctcga aatcgcgatc gacgaactgg cggaaaaagc gggcatcgat
    11281 cccgtcgagt ttcgcatcct gaatgacact caggttgacc ccgccgaccc gacgcgccgc
    11341 ttctctcgcc gtcagcttat cgagtgcttg cgcaccggag cggataaatt tggctggaag
    11401 cagcgcaacg ccacacccgg acaggtgcgc gacggggagt ggctagtcgg ccacggcgtc
    11461 gcggcgggct ttcgcaataa tctgctggaa aaatcggggg ctcgggttca cctcgaacca
    11521 aacggcaccg ttaccgtgga aacggacatg accgacattg gcaccggcag ctacaccatt
    11581 ctggcccaga cggcagcgga aatgcttggc gtaccgctgg agcaggttgc ggttcacctc
    11641 ggcgattcca gtttcccggt ttctgcgggt tctggtggac aatggggcgc gaatacctcc
    11701 acctccggcg tttacgccgc ctgtgtgaag cttcgcgaaa tgattgcctc ggcagtcggg
    11761 tttgatcctg agcagtcgca gtttgccgac ggcaagatta ccaacggtac ccgaagcgcc
    11821 atgctacatg aggccaccgc aggcggcaga ctgatagcgg aagagagcat tgaattcgga
    11881 acactgagca aggagtacca gcagtcgacc tttgccgggc attttgtgga ggtcggcgtg
    11941 catagcgcga cgggagaagt tcgggtccgg cgtatgctcg ctgtgtgtgc tgcaggacgc
    12001 atcctgaatc cgaaaactgc acgcagccag gtcattggcg caatgactat gggcatgggc
    12061 gcggcactga tggaggagct ggcggtggat gaccgtttgg gctacttcgt taatcacgat
    12121 atggcggggt atgaggtgcc ggttcatgcg gatatcccaa aacaggaggt gattttcctg
    12181 gatgataccg accccatctc ctccccgatg aaggccaaag gtgtcggtga gctgggcctg
    12241 tgcggcgtga gcgcggctat cgccaacgcg gtgtataacg ccaccggtat tcgggtacgg
    12301 gattatccca tcactctgga taagctgctc gataaactgc cggatgtggt ttaaggagga
    12361 acaatgtcat actcgctttt tgacaaagac gagcactggc ataagccaga gcaggcgttt
    12421 ctcaccgatg accaccggac cattctgcgc ttcgccgtag aggcgctaat gtccggtaaa
    12481 ggagcggtgc tggtgacgct ggtggagata cgcggcggcg cggcgcgccc gctcggggcg
    12541 cagatggtgg tgcgcgaaga tggtcgttac tgcggttttg tctctggcgg ctgcgtggag
    12601 gccgctgccg cttttgaagc gctggagatg atgggctcag gccgcgatcg cgaaattcgc
    12661 tatggcgaag gttcgccgtg gtttgacatc gttctgccct gcggcggtgg gatcacgctg
    12721 acgctccata aactacgctc ggcacagcct ctgctcgccg tgctgaaccg tctggaacag
    12781 agaaaaccgg cggggctgcg ctacgatccg caagcacaat cgttggtgtg cctgcccacg
    12841 aaaacccgaa cgggctggaa tctcaatggc tttgaggtgg ggttcaggcc atgcgtcagg
    12901 ctgatgattt acggacgttc tcttgaggcg caggcaaccg cgagtcttgc agcagccaca
    12961 ggctatgaca gccatatctt cgatcttttt ccggcctcag ccagcgctca gatcgatacc
    13021 gatacggcgg tcattttgct gtgccatgat ctcaaccggg agctgccagt gttgcaggcc
    13081 gcgcgagaag caaaaccctt ttatctcggc gcattgggca gctatcgaac ccacacgtta
    13141 cgtctgcaaa agctccacga gctgggatgg tccagggagg aaacaacgca aatccgggca
    13201 cctgtcggga tattccccaa agcccgggat gcgcatactc tggcactctc cgtgctggca
    13261 gaagtcgcct ctgtacgtct ccatcaggag gaggattcat gcctgccccc gtcgtcctga
    13321 tccttgcggc cgggcgtgga gagcgctttc tcgcctccgg tggaaatacc cataagtgta
    13381 tcggctggcg tcagtccccg gaggttgcgc cttatcgctg gccatttgaa gaaaacggga
    13441 gaactttcga ccttgcgatt gaaccgcaga ttacgactaa tgatctgcgt ctgatgttga
    13501 ggctggctct agccggcgga ggaataacaa ttgccactca ggaaactttc aggccatata
    13561 ttgaaagcgg taagcttgta tcgctgcttg atgactttct tccacaattt ccgggcttct
    13621 atctgtattt cccacagcgt cgcaatattg caccaaagct ccgcgccctg attgactacg
    13681 tcaaagaatg gcggcagcaa ttggcttaaa tgtctgcacc tgcactgcct gatgtcagaa
    13741 cagtattttg atgaattgcc aaggttacaa tggcacaaat acggcacagg aggaaacgtg
    13801 gtgtatttaa atatggggta actctttgat tttaatggtg c
//
LOCUS       DAPHBJ010000112        13752 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.113, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000112 DAPHBJ010000000
VERSION     DAPHBJ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.113"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..871)
                     /gene="yhaJ"
                     /locus_tag="RHZ76_002948"
     CDS             complement(<1..871)
                     /gene="yhaJ"
                     /locus_tag="RHZ76_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HDV2813551.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLRE"
     gene            976..1677
                     /locus_tag="RHZ76_002949"
     CDS             976..1677
                     /locus_tag="RHZ76_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HDV2813552.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1700..1864
                     /locus_tag="RHZ76_002950"
     CDS             1700..1864
                     /locus_tag="RHZ76_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813553.1"
                     /translation="MSKKLAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLVEDEATA"
     gene            complement(1925..3235)
                     /locus_tag="RHZ76_002951"
     CDS             complement(1925..3235)
                     /locus_tag="RHZ76_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDV2813554.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3263..4594)
                     /locus_tag="RHZ76_002952"
     CDS             complement(3263..4594)
                     /locus_tag="RHZ76_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDV2813555.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(4869..6233)
                     /gene="tdcG"
                     /locus_tag="RHZ76_002953"
     CDS             complement(4869..6233)
                     /gene="tdcG"
                     /locus_tag="RHZ76_002953"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDV2813556.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6305..6694)
                     /locus_tag="RHZ76_002954"
     CDS             complement(6305..6694)
                     /locus_tag="RHZ76_002954"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HDV2813557.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6708..9002)
                     /gene="tdcE"
                     /locus_tag="RHZ76_002955"
     CDS             complement(6708..9002)
                     /gene="tdcE"
                     /locus_tag="RHZ76_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HDV2813558.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9036..10244)
                     /gene="tdcD"
                     /locus_tag="RHZ76_002956"
     CDS             complement(9036..10244)
                     /gene="tdcD"
                     /locus_tag="RHZ76_002956"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HDV2813559.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGLEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10270..11601)
                     /gene="tdcC"
                     /locus_tag="RHZ76_002957"
     CDS             complement(10270..11601)
                     /gene="tdcC"
                     /locus_tag="RHZ76_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HDV2813560.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11623..12612)
                     /gene="tdcB"
                     /locus_tag="RHZ76_002958"
     CDS             complement(11623..12612)
                     /gene="tdcB"
                     /locus_tag="RHZ76_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HDV2813561.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGKI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(12711..13649)
                     /gene="tdcA"
                     /locus_tag="RHZ76_002959"
     CDS             complement(12711..13649)
                     /gene="tdcA"
                     /locus_tag="RHZ76_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HDV2813562.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3554 a         3717 c         3239 g         3242 t
ORIGIN      
        1 tttcccgcag acaccaggat ttcgcttccc ccatactgtc acgacgccag gccataataa
       61 tatcgatctc gctggtcgat tccgggctga cgacacgcaa ccgcccttcc gcaatatctt
      121 tttcgaccat aggatacggc atcgtcgcca cgccaagccc cgccagtaat gcctgacgtt
      181 tatcttcaat cgtgctcacc gttaagcgcg gctgtttgtc cagcagctgt acggtcaaca
      241 ccgggcgctc acgagcggta tccgccaccg caattccacg atatttcaca cgcgtcactt
      301 cagataacgg ttccggctcc tgatgaatcg ggtgatcagg cgcggcaacg tagacgttca
      361 ttaacgtata gagcttgcgc gagttgatct ccgacgagga acgaaaatgc atatccggcg
      421 cgataacaat atccgcccgc ccctgctcca gccgttccca cgcccccgcc agcacttctg
      481 tgatgattgc cagttgggta ttggcttttg ccgccagttt gtcgattaac gggaaaaagg
      541 caggtgtcgg taccagcgct tcggtcacaa tggtgagatg cgtttcccaa ccgcgcgcga
      601 gagcttccgc atcagtagtc agtttatctg cggcttccag caaaacgcgc cctcgctcca
      661 gcaacatcct cccgacattg gtgaatttgg tacgatggcc cgagcggtca aacagcacca
      721 catccagctc ttcttccagt ttttgcatgg tgtagctaag tgcggaaggc acgcgtccca
      781 gctcatccgc cgccgccgca aaactgcccc ggcgatcgat cgcatccata acccgtagtg
      841 cttccagcgt taatgccctt tctttggcca tttcgttctc attcaggaaa tttgaacata
      901 ccgggcagaa tatctggcta acaatgtagc gtccagcccc ttaccataaa aggaagtaaa
      961 gagaggtcaa gaattatgat tactacccga actgccaggc agtgtggaca agcagactac
     1021 ggatggttgc aggcccggta tactttttcc tttggacact acttcgaccc gaaattgtta
     1081 ggctatgcct ccctgcgtgt gcttaaccag gaagtgctgg ccccaggtgc cgcctttcag
     1141 ccgcgaacct atcccaaagt cgatatttta aatgtgattc tggatggaga agcagagtat
     1201 cgcgatagcg aaggcaatca tgttcaggcc agcgccggag aggcgttgct gctctctacc
     1261 cagccgggtg tcagctatag cgaacacaat ctcagcaaag acaaaccgtt aacgcgaatg
     1321 cagctttggc tggacgcctg cccgcagcga gagaatccgc tgattcaaaa gctggcgctt
     1381 aatatgggca agcagcaatt aatcgcctcg ccagagggtg cgatgggaag cctgcaatta
     1441 cgccagcaag tgtggctgca ccatatcgtg ctcgacaaag gcgaaagtgc gaatttccag
     1501 ttgcatgggc cacgcgcgta tttgcaatcg attcacggga aatttcatgc gcttacgcat
     1561 catgaagaga aagcggcgct gacctgcggt gatggggcgt ttattcgtga cgaggctaac
     1621 attacgctgg ttgccgattc cccactgcgc gctttgctga tagatttgcc tgtctagttg
     1681 tttttacagg gagatgatga tgagtaaaaa attggccaaa aagcgccagc cggtgaagcc
     1741 cgtggtggcg aaagaacctg ctcgcaccgc caaaaatttt ggctatgaag agatgttgag
     1801 cgagctggaa gctatcgtcg cggatgctga aacgcgttta gtcgaggatg aagctaccgc
     1861 gtaaagacaa gttgccggat gcggcgaaaa cgccttatcc ggcacaatac ccaaaaccta
     1921 gcgtttatct ggccttgctc gccataatct cgataatctg ccgatcggtt tgctgcatcg
     1981 aatggcttgc taacgcacac aggttggcaa tcgactgctc aacatcatgc gccacgatcc
     2041 cttcattgcc ggtcacggcg gtatcatcca gcgccattaa caccgctttc cacgcagccg
     2101 aagcactggt cgaaaccttc atcgcacagc tgttcgacgc tccatcgcaa atcatgccgc
     2161 tgacatcgcc gatcatactg ctgatcgcca tcgagatagt ttcataacgc ccatccacca
     2221 gccatgccat cccggcggcg gcccccattg ctgcggtcgt tgcggcacaa agtgcagaca
     2281 aacgcggtaa ctggttatgg atgtaaattg cgctcaaatg cgaaagcatc agcgcacgcg
     2341 ccagccgttc atcatccgct ccgaagtgtt ctgctaccac caccacaggc attgttgcgg
     2401 tgatcccctg gttacccgag ccagagttac tcattgccgg aagcgtagcg ccgcccatac
     2461 gcgcatcgga tgccgcgctg gtacgaatca caatggatga agagagatct ttcgccagca
     2521 aaccgcgcgc gcactgtttt tccagcgtcg cgccaatatg cagcccccac ttaccgctca
     2581 aaccttcctg cgataacgca caatttagct tcgcagaatc gagaataaag cggatcgccg
     2641 caaacgggac ttcattgacg aacttcagga tctcagccag cgtcgttctg gaaagcaccg
     2701 tcagcggaga ctcttgctcg ccctctgcca cacacgcctg ctgggtaaac accacaccat
     2761 cgtgcgtctc aatatgcaca atgttggtat gcccgccgac gatggtgaca cacgcccact
     2821 tctcaccgtt ccagactttg gcgcgtgaga agaggatttc attgcaaggt tcctggatct
     2881 taacggagac tttccccgcc gccagcagtg ctttggcatc ggcaattgcc tgcgcagttg
     2941 cgtctttcag cacttccagc ccggcgttgg catttccacc taacgccccc agcgccgccg
     3001 caatcggcag ccccaccatt cccgtgccgg gaacggtgac gcccagaccg ttcttcatca
     3061 gatttggcga aacccaggct tctacacgtt caaccggacc ttccagttct gccgcagcaa
     3121 ccgccgccgc cagcgccagt gaaatcggtt cagtacatcc cagcgccggt tttacttcct
     3181 cctgaacggc gaggatgtaa cgctgccata acggatttaa agtcgaatca aacatattaa
     3241 aaaccttaaa atttcaggta aatcaagaaa atgccaggaa cggagaaaca caaagcaaca
     3301 aaccagtgac gataatcagg tacagagaca tccctttgta tttgtgcaat gccggtactt
     3361 tgtaaaccag ccacgccggg atcaggcacc ctaccatgcc gaaaatcggg ctacagatag
     3421 aggtgaagct caacaccggt gcgttcagta cgatggcgct ccaggccagc aaaatggcga
     3481 aaatcatgat gccgcgctga acaagatttt cgttaatctt ctcggcaggc atcttgcgac
     3541 gcaggatgtt cattacgatc ccttgcgttg cttcgcgaaa gcctaaatag acgccaaaga
     3601 atgcagtcat gacagcaaag atattgagaa tgacgctgac cactttcacc cacgctgcgc
     3661 cgtcaccgct aataaactgt gcggcaatcg ccagcgcgga aatattctgc tcgtaggctt
     3721 ttaccgcttc gtcatgtccc atcgccagcg tgaacgacac ggcgtagaaa aagacggtga
     3781 caaacaaaat gccaaacgcg atattcattg cccgcaatgc tttatgccgc gccacttcaa
     3841 ttgatttttc ccgcgagcga taagagatca ccatcgggct taacgtctgg ataaacagaa
     3901 tcgacgtcag ggtaaacggc agcgtaataa tggcgttttt caccagcagc cccagcggcg
     3961 gtagcgaacc gacgttatac agatgccaca ttcccaccat cgacacgccc agcgccgcga
     4021 ccaccagcag cttggtcagc accatgccgg tcgaaatttt gaataacaat ttctcgccgc
     4081 gtgaagagat cgccaccaga atgcaaatca gtaccagacc ataaaaggga ctgtctgaca
     4141 gcaacccttc cgtcacgccg aaggtatgca gataggaagc actatcgttg gtgatggcgg
     4201 tggaatagac gaacatccaa atcaccagca ttacgaaata gagcgcacct aacaggatgc
     4261 cccagttttt acctaaataa ccgctaatga cactcgggta atctttacat tctggtgatt
     4321 ctgccagcgt attaataaac aaccgctgaa acagatacat tgccgggtaa ccaatcaccg
     4381 atgagagcaa aaatacccac aatcccatca aaccgacctg caccgggaga aaaacaatcc
     4441 ccgcgccaat cgccatcccg atactcataa tcacccagcc ggtgtcagta ctgtcgaatt
     4501 tgatcgcttc tcgccactcg ctctcactca ttccagcacg acccgccggg gtcgaagcgt
     4561 ctgcaatgac gcctttattc gatgcaattt ccataatttc tcgctcaaaa ttttgtaggg
     4621 cttattattt ttttccgagc cgcatcaagg cgatatgcgg tctacgtttt tgcaggcgag
     4681 ttattagaat aaagaaatga tacgcgcgag gtcagagaaa atcttcacaa tcaagacttt
     4741 gaaataacgg agatggataa gaattttcta ctaaattaat cgcaagagaa tattcattct
     4801 ctatttgcgg cagatcacaa aaaaaaggtg cacatttgtg cacccaagga tgaaagctga
     4861 cagcaatgtc agccgcagac cactttaatg gccagtcctc cgcgtgatgt ttcgcggtat
     4921 ttatcgttca tatctttgcc ggtttcatac atcgtctcga tcactttatc gagtgaaaca
     4981 cgcggtgccg aggtgcggcg catcgccatc cgcgcggcgt ttactgcttt cacggcatta
     5041 atggcattac gttcaatgca cgggatttgt acctgtccgg caaccggatc gcaggtcagc
     5101 ccaaggttat gctccatcgc gatttccgcc gcattgcata cctgcgccgg actgccgccc
     5161 agtagttcag ttaaccctgc cgccgccatt gaacaggcca cgccaatctc cccctgacag
     5221 ccgacttccg cgccagagat ggaggcgttc attttataca gcgcgccaat agcccccgcg
     5281 gccagaaaat agcgggcaat tgaccgctcg tttaccggac gacggaactt atcgtaataa
     5341 gccagtactg ccggaataat gccgcacgca ccgttagtcg gtgccgttac cacgcgcccg
     5401 ccagctgcgt tttcttcact aaccgccagc gcgtacatgt tgatccagtc gatgacattc
     5461 atcggatcgt tagagatgtt atcgctggaa accagctgac gacgcagcgc tacggcacgg
     5521 cgcggcacat tgagcggacc aggcagcacg ccttcagtgt tcatcccacg ttcaataccg
     5581 tcatgcatca cttgccagat acgggcaaaa ccggcgtcaa tttccgcttt gctgcgcagc
     5641 gctagctcgt tgtgcatcat cagaccagat atagacaggc cgttgtaatc acacattttc
     5701 agcagttcac ctgctgagtg gaaatcgtaa ggtacggacg tttcgacatc gtgcgacagg
     5761 ccgaagtgtt cttcttcgac aataaacccg ccgccgacag agtaataggt tttacttaat
     5821 agctcttcct gtcccttcca ggcagtgatc cgcattccgt tctcatggcg aggcaacatt
     5881 tcgggatgga agataatgtt ctttgctaca ggaaaatcaa caatatgcgc accagatgcc
     5941 actggcagcc gcccgctgcg cgttactaac tctataaatg cagggatctc atcaatgaca
     6001 acatcctgcg gactgtttcc tgccagtccc atgatgatgg cgacatccgt ggcatggcct
     6061 ttgcccgtca gtgacaacga cccgtacaga tcgaccacaa tatggctcgt cgcggttaat
     6121 aagccgctac tttccagccg atcaataaaa ctttttccgg cattcattgg ccccacggta
     6181 tgcgaactgg agggaccaat cccaattttg aaaatatcga atgcactaat catatccaca
     6241 ccctcggatt gccgttcagt gaagtggagc ggaacgacct tacgaccgtc ccgctcacga
     6301 ggctttacgc actacgtact gcgatggctt caatttccag cttcacatct ttcggcaaac
     6361 gcgcgacctg cacacagctc cgggtcggat aggtcgcctg atgctcatcg aagaactgct
     6421 tatagacttc gttgatggtg gcaaaatcat tcagatcggt gataaacacg gtcatcttga
     6481 tgatatcgcc cacgctcagc ccggcagcaa ccacgatcgc tttgacgttt tcgaggctta
     6541 aacgcgcctg atcttgcaca tcagccggga tctcaccggt ctgtgggcaa accggtattt
     6601 gcccggaggt gaagaccatg ctgcctaaat caacgccctg aacataaggg ccgattgcgc
     6661 ctggggcacg ttgcgtttcg ataatctttt tcatacatcc tccggcgtca gagcgcctgg
     6721 gtaaaggtac gtgaaataac atcctgttgc tgttcacggg tcagtgcgtt gaagcgcacg
     6781 gcgtagccag agacacggat tgtcaggtta ggatattttt ccgggtgctc gatggcatcc
     6841 agcagcattt cccgattcat tacgttgacg ttgaggtgtt gaccgccttc gacatccgct
     6901 tcgtggtgga aatacccatc cagcaggccg acaaggttgg ttttacgtac tggatcttct
     6961 ttgcccagcg ccgcaggaac gattgagaag gtgtacgaga tcccatcttt ggcgtaggtg
     7021 aacggcagtt tcgccaccga cgtcaatgag gccacggcac ctttgcggtc acgaccatgc
     7081 atcgggttag cgcccggcgc gaacggtgtt ccggcgcgac gaccgtccgg cgtattaccg
     7141 gttttctggc cgtacaccac gttagaagtg atagtcagaa tcgactgggt agggacggcg
     7201 ttgcgatagg ttggcagcgc tttaattttc ttcataaagc gttcaaccag gtcgcaggca
     7261 atgctgtcta cgcgctcgtc gttgttgccg tactgcggat attcaccgtc gatttcaaag
     7321 tccaccgcca ggccgttttc gtcacggatt ggtttcacgc gggcatattt gatggcagac
     7381 agggagtccg tcgccaccga caggcccgcg atgccgcatg ccatagtgcg atagacatca
     7441 cgatcgtgca gcgccatcag cgaagcttcg tagctgtact tgtcgtgcat gtagtgaatg
     7501 atattcagcg cgctgatgta ctgcaccgcc agccagtcca tgaagtgatc gaggctgtcc
     7561 atcactttgt cgtaatccag cacgtcgtcc atcagcggtg ctgttttcgg cccgacctga
     7621 atcttcagct tctcgtccac cccgccgtta attgcgtaga gcagcgtttt cgccaggtta
     7681 gcgcgtgcac caaagaactg catttgctta ccaatcacca ttgggctgac gcagcaggca
     7741 atcgcgtaat cgtcgctgtt gaagtcagta cgcatcagat cgtcattttc atactgcaag
     7801 gaagaggtga cgatcgacac ctgcgcggca tattttttga aggcaatcgg taattcttcc
     7861 gaccaaagaa tggtcaggtt aggttccggt gccggcccca tagtgtgcag ggtgtgcaaa
     7921 tagcggaagg agtttttggt caccagcgta cgaccgtcca gccccatccc gccgatcact
     7981 tccgtcgccc agattgggtc gccggagaac agcgaatcaa attccggtgt acgcaggaag
     8041 cgtaccatac ggatcttcat gatgaagtga tcgatcagtt cctgtgcctg ctgctcattg
     8101 agtacgccag ctttaaagtc gcgctcaatg tagatatcga ggaacgatgc cgtgcggccc
     8161 agcgacatcg cgccgccatt ttgcgatttc actgccgcca gataagcgaa gtagagccac
     8221 tgcaccgctt cctgcgcatt ctgcgccggg cgagagatat caaagccata tttcgccgcc
     8281 atttcctgaa tctgcaacag cgcatgacga tgctctgcca gctcctcacg cagacggatg
     8341 gtggcttcca gatcctcgcc tttttccaga cgagactgga gatcggcaaa ttgcagttcg
     8401 cgttcacgta ccagataact gataccatac agcgctacgc ggcgatagtc accgataatg
     8461 cgcccacggc catagccatc tggtaaaccg gtcagcacgc cagatttacg gcagcgcagc
     8521 atatccggtg agtaaacatc aaatacgccc tggttatggg ttttacgcag atcggtaaac
     8581 agatattcaa attcactgtc catttctcgg ccataggcgt ggaatgaact tttaatcata
     8641 ttaatgccac cgaacgggtg tagcgcacgt ttcaacggcg catccgtttg caggccaaca
     8701 attttttcca gcggctggtt aatatatccc gcatcatgag cggtaattgt ggtggcaata
     8761 ttggtatcga aatcaaccgg cgcgtgggtt gcattttcga tacggatgcc ttccattact
     8821 ttttcccata attccgtggt ggcaggcgtc gcttcggcga ggaaagattc atcgccttca
     8881 tacggtgtat agttatgttg aataaaatcg cggacattaa tttcgttttt ccagtccgta
     8941 cctttaaagc caagccatgc gtcggcgtac agcttatcgc tggtatcaat atctaccttc
     9001 atgaaaaata atctctctac aatacttcaa ctaaattatg caaattctgc gggcgcgtta
     9061 actttgccta aatgaatggc atccaaagca atcatttttt cttcgttagt cggaataacg
     9121 gcacaaatga cacgcgcatt ttcactggaa acaattcgct caccacagga gttagagcga
     9181 ttattcattt ctgtatcaat ctctaagcct aatacagcca aatgttccat gaccagacga
     9241 cgaattaagc ttgaattctc tcctattccg ccggtgaata taattccatc caggcgacgt
     9301 aatgaagctg cgtgtccggc aatatgacgg gcaattcggt gaacaaaggt tttaattgcc
     9361 agttgcgcgc gttcgtgacc ttcatgccag gctttttcca gaacacgtaa atccgaagaa
     9421 agaccggaaa tacctaataa tcccgactct ttattcacta cgcgttccag gtcacccagg
     9481 ctctggttgg tttggctggc gacccaggac atcgcaccaa agtcgacatc gccactgcgg
     9541 gtacccatca tcaagccttc cagcggcgtc attcccattg aggtatcaac actctgaccg
     9601 ttgcgaaccg cgcagattga cgcgccattg ccaagatgcg ccacaaccag gccggagtca
     9661 tcttccgcca gattcagcag cgaatgtgcg cgctgggaaa catagcggtg cgacgtgccg
     9721 tggaaaccat aacggcgtac acctaactct tcataatatt tccacggcag gccgtataaa
     9781 taagcttccg gagccatcgt ctggtggaaa ctggtatcaa ataccgccac ctgagttacg
     9841 cccggaaata attgctgcgc cgattcaata ccacttaaat tggcgtaatt atgcaggggt
     9901 gccagtggag aaacgcgacg gatattatca atgacttcat cggtaataat ggcggactcg
     9961 gtaaaaatac tgccgccgtg agcgatgcgg tggccaatta aggccacact gtcatttaaa
    10021 ttccgttttt ccagttcaaa tgcaattgcc ttcaatgcac cttcgtagct gtggtgagcc
    10081 agcggtgctg gctctccccc atttacggat aagaatgcat tttccgagtt aataccgtcg
    10141 gcaatacctg acattaatac ttcacagtcg ctggcatcga gcacggaaaa cttaatcgaa
    10201 gacgaaccac agttaataac caaaacaacc ggaaattcat tcatctcttt tctcatcctg
    10261 agttacggat taaaacagtt tgtatacgat gttcaggatg gtcagcagac caatcacggt
    10321 aacaaacacg ttatccagac gaccacggta tttcgccaga gacggcgctt tacggatggc
    10381 atacatcggc aacaggcaca gcagggatgc gataatcggt gcgcccatgg cttcaatcag
    10441 gtcaaggatg ttcgggttgg cgtaggcaac aacccaggtg gagcccatga tgaagatcat
    10501 gctgatagtg ttcagtttac ccagcgacac tttagttttg tcgcctttat aaccaaactt
    10561 caggaccagg ccattcagac cttccagcgt tcccagatag tgaccgaaga aagatttgaa
    10621 gatagccacg agtgcgatga tggaagccgc atattccagt gtaatcgcga acgttgtttt
    10681 ggtaccggtc atggacgcaa agtggttagc cagataagaa agcactggaa tattctgcgc
    10741 tttggcttcc gccatgttgg ccggagacag agtaaacagg cagctaaagg caaagaacat
    10801 caccactgca accatcagca tgctggcacg agaaatgatt tgggaacatt tacgttcggt
    10861 gaagtcgcga ccgaagtctt tctcatactc ttcacgctta gaaaccacga aggaagagac
    10921 gattggcgag aagttaaagg agaaaaccat gatggaaatc cccagccaga cagtgatcag
    10981 gataccgtca tgaccggtta acgacagcga accgaggtca acctggtcga taactgcaga
    11041 gttccagtaa gggatcagcg acaaagaaat cagcaccagg ctggcgataa acggccatac
    11101 caggtagctc atcactttaa ccatcagatc cttaccaaac cagatgacga aagccatcag
    11161 cagcaacagg aacagcgcca caaagccgcg attcagcggt gcaaagccga gctggttttc
    11221 ccagaacgtc ataaaggtat tggtaatagt aacgccataa atccacagca gtgggcaaat
    11281 cgcgaagaag tacaggaacg tgataaccac gccgccagtt ttaccaaaat gctcttccac
    11341 cgtttccgta atgttgccgg aagggttaga gccagaaaga cacagacgcg ccagcgcccg
    11401 gtggcaataa aacgcgatgg ggtatgccaa taccaacatc agaagaatcg ggatcagtcc
    11461 gccaaaacct gcgcggatag ggaagaacag caccccggcg ccgattgccg taccaaacaa
    11521 gcctaacgtc catgtggtat ctgatttacg ccaggacgat tgttttgtct ggctggatac
    11581 aatgctatct gaagtactca tatcctatcc tcaacgaatt aattaagcgt caacgaaacc
    11641 ggtgatttga gagacgcgag aaagatcgat attgccgccg gaaataatac tgacggtttt
    11701 tctgttttga atatattggt ctaatttacc gcttaataat gcagcacatg ccagagcgcc
    11761 tgcgccttcg gtgacgactt tattgcgctg aattaaggca atcatactgt ttctgatttc
    11821 gtcttcgctg accagcacga tgtcatcgac taattcacga acgatttcgt aagttaaatt
    11881 acccggacgg gagacatcac aaccatccgc cagggtgccg gtagttcggt gcgtggttat
    11941 ttctccggag tggaaagaag ccgccatgcc gtgaacgttt tcagactgta cgccaataac
    12001 acgaatggtc gggttaatag atttaattgc caccgcaata ccagcaatta aaccgccacc
    12061 accaattggc acaatcacgt tatcgacatc atagagatct tccataattt ccagaccaat
    12121 cgttccctgg ccagcaatca ctttcggatc atcgtaaggt gggataaaaa tacggccttc
    12181 catttcgaca atttcgctca ctttagcgat agtgtcgttg aagttatcac catgcagaac
    12241 gacttctgcg gagtagtcgc acgttgccgc tactttggat tttggcgcac cttttggcat
    12301 caccacttta ccgtcgatac ccagcatcgc gcaggagagg gaaacccctt gcgcatggtt
    12361 gcccgcagaa caggccacca cgcctttgcg tttttccgca tcggtcagtg aacttaattt
    12421 attaaatgcg ccacgaattt taaatgaacc cgtacgctgc atattttcaa acttcaggaa
    12481 tattttacct ttgcaacgtt cactaaaata gttggagcga ggcatgcctg ttttataaat
    12541 tcgcccagcc agtcgttgtt tcgcttcaat aatgtcatca atagcaaccg gcagatcgta
    12601 tgtaatatgc attataaaac ctcttcgcct gaattaaata tgtaggtaac cgtaaccgac
    12661 acctgcaaga cagacaggtg gattatttat gtttaaataa aaacaaataa ctaaccaact
    12721 tctattaatt gccttcgtct acacccatta taagatgaat actctttggc taattccacc
    12781 aaaaccgatg ctgctttttt aatacgataa tttttcgacc ataccgcggc atattgtgcc
    12841 acaggtaatg tttcttcaac cggaatagta ataaattgat tagaaccaaa aggtgacgtc
    12901 atatcacaag gaattacagt taagaaatca gcattgagaa caagattata aattgtcacg
    12961 actgagtcgg ttttaacgat gttttcaata ctgatgccat ttctttgtaa cgtagtaagc
    13021 agttcgctgt agtaccccat attagtttgt ggcaacaccc actgttcgtt cttcaacgac
    13081 tccagcgtgg tggtgccggt gcatgttcgg gacttactgg ctaccagcac aaactcggac
    13141 tcgaacagcg gctcaacatg taaatcctga agcttcattt ctgcacttaa cgtaccaatc
    13201 gcaaaatcca gtcgaccgtc gcggattgcc ggtaagaacg aagacagttg cgcttcatac
    13261 atagaaacct gcgctttcgg gaacacctct ttgaacttgt tgatcatccc tgacataaaa
    13321 gtaaaaccaa tcaatgaagg aaaaccaaat gagacttcca ccaccgcctc agaagacata
    13381 ccgcttatct cattaaccat atttttcatt tcacgggtaa tggattcgga acgggagagt
    13441 aacaattgac cggcaggtgt taatgttaca ccggtatttt tccgcaccac taattccaca
    13501 ccaaaataat cttcaatatc gttaatgatt ttactgacgg ccggttgagt taaccctaat
    13561 tcttttgcag ccgagccgat agaaccactt ctaatgactt cctgaaagac taccaggtgc
    13621 tgcgttttcg gaagaagaat agtgctcata acgacctacg ttaattacct cattgacggc
    13681 atgaagtgta tcaaaatgaa atgaacagga tatgtgcgac cactcacaaa ttaactttca
    13741 atactttcca ga
//
LOCUS       DAPHBJ010000113        13692 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.114, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000113 DAPHBJ010000000
VERSION     DAPHBJ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.114"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            187..681
                     /gene="napF"
                     /locus_tag="RHZ76_002960"
     CDS             187..681
                     /gene="napF"
                     /locus_tag="RHZ76_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HDV2813563.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYDCAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            671..934
                     /gene="napD"
                     /locus_tag="RHZ76_002961"
     CDS             671..934
                     /gene="napD"
                     /locus_tag="RHZ76_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HDV2813564.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            931..3417
                     /gene="napA"
                     /locus_tag="RHZ76_002962"
     CDS             931..3417
                     /gene="napA"
                     /locus_tag="RHZ76_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HDV2813565.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIRWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVDTRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3424..4119
                     /gene="napG"
                     /locus_tag="RHZ76_002963"
     CDS             3424..4119
                     /gene="napG"
                     /locus_tag="RHZ76_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HDV2813566.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4106..4969
                     /gene="napH"
                     /locus_tag="RHZ76_002964"
     CDS             4106..4969
                     /gene="napH"
                     /locus_tag="RHZ76_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HDV2813567.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            4966..5415
                     /gene="napB"
                     /locus_tag="RHZ76_002965"
     CDS             4966..5415
                     /gene="napB"
                     /locus_tag="RHZ76_002965"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HDV2813568.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5425..6027
                     /gene="napC"
                     /locus_tag="RHZ76_002966"
     CDS             5425..6027
                     /gene="napC"
                     /locus_tag="RHZ76_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HDV2813569.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6046..6663
                     /gene="ccmA"
                     /locus_tag="RHZ76_002967"
     CDS             6046..6663
                     /gene="ccmA"
                     /locus_tag="RHZ76_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HDV2813570.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6660..7322
                     /gene="ccmB"
                     /locus_tag="RHZ76_002968"
     CDS             6660..7322
                     /gene="ccmB"
                     /locus_tag="RHZ76_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HDV2813571.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7364..8101
                     /gene="ccmC"
                     /locus_tag="RHZ76_002969"
     CDS             7364..8101
                     /gene="ccmC"
                     /locus_tag="RHZ76_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HDV2813572.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8098..8307
                     /gene="ccmD"
                     /locus_tag="RHZ76_002970"
     CDS             8098..8307
                     /gene="ccmD"
                     /locus_tag="RHZ76_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HDV2813573.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRDAQQQEAA"
     gene            8304..8783
                     /gene="ccmE"
                     /locus_tag="RHZ76_002971"
     CDS             8304..8783
                     /gene="ccmE"
                     /locus_tag="RHZ76_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HDV2813574.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8780..10723
                     /gene="ccmF"
                     /locus_tag="RHZ76_002972"
     CDS             8780..10723
                     /gene="ccmF"
                     /locus_tag="RHZ76_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HDV2813575.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10720..11277
                     /gene="dsbE"
                     /locus_tag="RHZ76_002973"
     CDS             10720..11277
                     /gene="dsbE"
                     /locus_tag="RHZ76_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HDV2813576.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11274..12326
                     /gene="ccmH"
                     /locus_tag="RHZ76_002974"
     CDS             11274..12326
                     /gene="ccmH"
                     /locus_tag="RHZ76_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HDV2813577.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVQEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKTDPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12537..13184)
                     /gene="narP"
                     /locus_tag="RHZ76_002975"
     CDS             complement(12537..13184)
                     /gene="narP"
                     /locus_tag="RHZ76_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HDV2813578.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13504..>13692
                     /locus_tag="RHZ76_002976"
     CDS             13504..>13692
                     /locus_tag="RHZ76_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813579.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGG"
BASE COUNT         2905 a         3467 c         4018 g         3302 t
ORIGIN      
        1 agcaaaagcc cgtttttgcc acgcttcccg ccactctttt gatcctgcta caggttttac
       61 cccgatcggg gtatgcatct ttgacacatc ctttaatatc ttagcggcta taaaaatggc
      121 ttattaatta tgcggtttat ttggtcgctc tcaattttca gagcgcgtta atgatggaag
      181 gtcaatgtga agattgatgc atcccgtcgg ggcatactca ctggtcgctg gcgcaaagcc
      241 agtaacggta tccgtccgcc ctggtccggt gatgaatctc attttctgac ccattgcacc
      301 cgctgcgacg cctgtattaa tgcttgtgaa aacaacattc tgcaacgcgg cgcgggcggc
      361 tatccgagcg ttaatttcaa aaataacgag tgcagcttct gttacgactg cgcgcaggct
      421 tgccccgaat cgctattttc tccgcgccac accagggcct gggatttgca gttcaccatt
      481 ggggacgcgt gcctggcgta tcagtctgtt gaatgccgcc gctgtcagga tagctgtgag
      541 ccaatggcaa ttatctttcg cccgacgctc tccgggatct accagccgca acttaatagc
      601 caactttgca acggatgtgg agcatgtgca gccagctgtc cggtatcagc cataactgcg
      661 gagtatctcc atgcacacta actggcaagt ttgcagcctg gtcgtgcagg ccaaaagcga
      721 acgaatttca gacatcagca cccaactgaa cgcctttccc ggctgtgaag ttgctgtcag
      781 cgacgcgccg agcggtcagt tgattgtggt ggtggaagca gaagacagcg aaacgctgat
      841 ccaaaccatt gagtcagtac gcaacgtaga gggcgtgctg gcggtgtcgc tggtttatca
      901 ccagcaggaa gagcaaggtg aggaaacacc atgaaactca gtcgtcgtag ctttatgaaa
      961 gctaacgccg ttgcggccgc tgcggcggct gccggtctca gcgtgccggg cgttgcccgc
     1021 gccgttgttg gtcagcagga agccatcaga tgggataaag cgccgtgccg tttctgcggt
     1081 actggttgcg gcgttctggt cggaacgcag cagggacgtg tggtggcctg tcagggcgac
     1141 ccggacgcac cggttaaccg tggcctgaac tgcattaagg gctatttcct gcccaaaatc
     1201 atgtacggta aagaccgttt gacgcagccg ctgctgcgta tgaaaaacgg taaatatgac
     1261 aaagaaggcg aatttacccc aatcacctgg gatcaggctt tcgatgtgat ggaagagaaa
     1321 ttcaaaaccg ccctgaaaga aaaagggccg gaatcgatcg gtatgttcgg ttctggtcag
     1381 tggactatct gggaaggtta tgccgcgtcc aagctgttta aagcgggctt ccgttcgaac
     1441 aacatcgacc cgaacgcacg tcactgtatg gcgtcggcag tagttggctt tatgcgtacc
     1501 tttggtatgg atgagccgat gggctgctat gacgacatcg agcaggctga cgcgtttgtg
     1561 ctgtggggcg ctaacatggc ggagatgcac ccgatcctct ggtcacgcat cactaaccgt
     1621 cgtctctcta accagaacgt caccgtggcg gtgctttcta cctaccagca tcgtagcttc
     1681 gagctggcgg ataacggcat catctttacg ccgcaatctg acttggtgat cctgaactac
     1741 atcgccaact atatcattca aaacaatgcg ataaatcagg acttcttcag caaacacgtt
     1801 aacctgcgca aaggggcgac ggacatcggc tacggtttac gtccgaccca tccgctggaa
     1861 aaagcagcga agaatccagg ttctgacgct tccgaaccga tgagctttga agattacaaa
     1921 gccttcgttg ctgagtatac gctggaaaaa accgctgaaa tgaccggcgt gccgaaagac
     1981 cagttagaac aactggcgca gctgtatgcc gatccgaaca agaaagtcat ctcctactgg
     2041 acgatgggct tcaaccagca tacccgtggt gtgtgggcca acaacctggt ctacaacctg
     2101 cacctgctga ccggcaaaat ttcccagccg ggttgcggtc cgttctccct gaccgggcag
     2161 ccttccgcgt gtggtactgc gcgtgaagtg ggcacctttg ctcaccgtct gcctgcggac
     2221 atggtggtga ctaacgagaa acatcgtgat atctgcgaga agaagtggaa tatcccgagc
     2281 ggcaccattc cggcgaaaat cggtctgcat gcggtagcac aagaccgtgc gctgaaagac
     2341 ggcaagctga atgtttactg gaccatgtgt accaacaaca tgcaggccgg gccgaacatc
     2401 aatgaagagc gtatgccggg ctggcgcgat ccgcgcaact tcatcatcgt ctccgatccg
     2461 tatccgacag tcagtgcgct ggccgccgac ttgatcctgc cgaccgcaat gtgggtagag
     2521 aaagagggcg cttacggtaa cgccgaacgc cgtactcagt tctggcgtca gcaggtacag
     2581 gcaccgggcg aagcgaaatc ggatctctgg cagttagtcc agttctcccg ccgcttcaaa
     2641 actgaagaag tatggccgga agagctgctg gcgaagaaac cggaactgcg tggcaaaacg
     2701 ctgtacgaag ttctgtatgc cacccccgaa gtgagcaaat tcccggtatc cgaactggcg
     2761 gaagatcagc tgaacgatga atcccgcgag ctgggcttct atctgcaaaa agggctgttc
     2821 gaagagtacg catggtttgg tcgcggtcac ggtcacgatc tggcaccgtt cgatgactac
     2881 cacaaagcgc gcggtctgcg ctggccggtg gtgaacggta aagaaacgca gtggcgttac
     2941 agcgaaggta acgacccgta cgtgaaagcg ggcgaaggct acaagttcta cggtaaaccg
     3001 gatggcaaag cggtgatctt cgcgctgccg ttcgaaccgg cggcggaagc accggatgaa
     3061 gagtacgacc tgtggctctc taccggacgc gtcctggagc actggcacac cggcagtatg
     3121 actcgccgtg tgccggaact gcaccgcgcc ttcccggaag cggtcctgtt tattcacccg
     3181 ctggatgcga aagcgcgcga tctgcgccgt ggcgacaaag tgaaagtggt ttctcgccgt
     3241 ggcgaagtga tctcgattgt tgatacgcgc ggtcgtaacc gtccgccaca gggcctggtg
     3301 tacatgccgt tcttcgacgc cgcacagctg gttaacaaac tgacgctgga tgcgaccgat
     3361 ccgctctcga aagagacgga cttcaagaag tgcgcggtca aactggagaa ggtgtaagcc
     3421 attatgtccc ggtcagcgaa acctcaaaat ggtcgccgcc gctttctgcg cgatgttgtt
     3481 cgcacagcag gcgggctggc tgccgtgggt gtggcgctgg ggttacaaca gcaaaccgca
     3541 cgcgcatctg gcgtgcggtt gcgcccgccc ggagccataa acgagaacgc ctttgccagt
     3601 gcctgtgtgc gttgcggtca gtgtgttcag gcttgccctt acgacacctt aaaactggcg
     3661 acgctggcct ctggtctgtc ggcgggcacg ccatattttg tcgcacggga tattccttgc
     3721 gaaatgtgtg aggacattcc gtgcgccaaa gtgtgcccga gcggtgcgct ggatcgtgag
     3781 atcgaatcga tcgacgacgc gcggatgggg ctggcggtac tggtggacca ggaaaactgt
     3841 ctcaactttc aggggctgcg ctgcgatgtt tgttatcgcg aatgcccgaa aattgatgag
     3901 gccatcaccc tggagctgga gcgcaacacg cgtaccggta agcacgcccg ctttctgccg
     3961 acggttcaca gcgacgcctg taccggttgc ggtaagtgcg aaaaagtatg cgtgctggaa
     4021 caaccggcaa tcaaggtgtt accgctgtca ctggcgaaag gggagttagg tcaccattac
     4081 cgcttcggct ggctggaggg gaacaatggc aaatcgtaaa cgtgacgccg ggcgcgaggc
     4141 gctggagaaa aaaggctggt ggcgcagtca ccgttggctg gtgttacgtc gcctttgtca
     4201 gttcttcgtg ctggggatgt ttttgagcgg tccatggttt ggtgtgtgga tcttgcacgg
     4261 caactacagc agtagcctgt tattcgacac cgttccgctg accgatccgc tgatgacgct
     4321 gcaaagtctt gccagtggtc atctgcctgc cacggtggcg ttgaccggtg cggtgattat
     4381 caccgtgctc tacgccctgg cgggaaagcg attattttgc agctgggtct gcccgctgaa
     4441 cccgattacc gacttagcga actggttacg caggcgcttt gacctcaatc agtctgcgac
     4501 gatccctcgc cacattcgct acgtgctgtt ggtggtcatt ctggtgggat cagccttaac
     4561 tggcacgctc atttgggaat ggatcaaccc cgtttctctg atggggcgta gcctggtaat
     4621 gggcttcggc agcggcgcgc tgcttattct cgcgctgttt ttatttgatc tactggtcgt
     4681 tgaacacggc tggtgcgggc acatttgtcc ggttggcgcg ctgtatggcg tgctgggtag
     4741 caaaggtgtg attaccgttg cggccaccga tcggcagaaa tgtaaccgct gtatggattg
     4801 ttttcatgtt tgcccggaac cgcatgtgct acgtgccccg gtgctggatg aacaaagccc
     4861 ggtgcaggtc accagccgcg attgcatgac gtgcggtcgc tgcgtggatg tctgttctga
     4921 ggatgtattt acaataacta cacgatggag ttcgggagcg aaatcatgaa aagccatgac
     4981 ctgaagaaag cgctgtgtca atggacggcg atgctggccc tggtggtaag cggcgcggtt
     5041 tgggcggcta atggcgtcga ttttagccag tcgccggaag tatccgggac acaggaaggg
     5101 gccattcgga tgccaaaaga gcaggaacgg atgccgctga actatgtgaa tcagccgcca
     5161 atgatcccgc atagcgttga aggttatcag gtaacgacca ataccaaccg ctgcttgcag
     5221 tgccacggtg tcgaaagcta tcgcaccact ggcgcgccgc gtatcagtcc tactcacttt
     5281 atggacagcg acggcaaagt gggcgcggaa gtggctccgc gtcgttattt ctgtctgcaa
     5341 tgtcacgtac cgcaggccga taccgcgcca atcgtgggga atacctttac cccatcaaaa
     5401 ggttacggga aataagaggt tattatggga aattctgacc gtaagcctgg tctgattaag
     5461 cgcctgtgga aatggtggcg tacccccagc cgtctggcgc tggggacgct gctgttgatc
     5521 ggttttgttg gcggcatcgt cttctggggt ggctttaaca ccgggatgga aaaagccaat
     5581 accgaagagt tctgcattag ctgccacgaa atgcgcaaca cggtgtatca ggaatacatg
     5641 gattccgtgc actacaacaa ccgtagcggc gtccgtgcga cctgtccgga ttgtcacgtt
     5701 ccgcacgagt ttgtgccgaa gatgatacgc aagctcaaag caagtaaaga gctgtatggt
     5761 aaaatttttg gcgttattga cacgccgcag aaatttgaag ctcatcgtct gacgatggca
     5821 cagaatgagt ggcggcgcat gaaggacaat aactcgcagg agtgccgtaa ctgtcacaac
     5881 ttcgagtata tggatacaac cgcccagaaa tcggttgccg cgaagatgca tgaccaggcg
     5941 gtgaaagatg ggcaaacctg tattgattgc cataaaggga tagcgcacaa gctgcccgat
     6001 atgcgtgaag tcgagccagg tttttaacag ggttattgcg tgggtatgct tgaagccaga
     6061 gagttacttt gtgagcggga tgaacgaacc ttatttagtg gcttgtcatt tacgctgaac
     6121 gcaggagagt gggtacaaat caccggtagc aacggcgcgg ggaagacaac gcttctccgt
     6181 ttgctgacgg ggttgtctcg ccctgacgca ggcgaggttc tctggcaagg gcagcccttg
     6241 catcaggtac gcgacagcta ccatcaaaac ctgttatgga taggccatca gccggggatc
     6301 aaaacccggc tgacggcgtt agaaaatctg cacttttatc atcgcgatgg cgataccgca
     6361 caatgtctgg aagccctggc gcaggccggg cttgccggat tcgaagatat tcctgtaaat
     6421 cagctctcgg ccgggcaaca acgccgcgtc gctttagcgc gtctgtggct gacccgtgcc
     6481 acgttatgga tcctcgacga gccttttacc gcgattgacg ttaacggcgt cgatcgtctg
     6541 acccagcgta tggcgcagca tacggagcag ggggggattg tgattctgac tacccaccag
     6601 ccgctcaacg ttgctgaaag taaaattcgc cgcatttcac tgacgcaaac gagggccgca
     6661 tgatgttctg gcgcattttc cgtcttgagc tgcgtgtagc gtttcgccat agcgccgaaa
     6721 tcgccaaccc gctgtggttc ttcttgattg taattaccct ttttccgctc agtatcggtc
     6781 cggagccgca actgctggcg cgtattgcac cgggcattat ctgggttgct gcgctgcttt
     6841 catccttgct ggcgctggaa cgactgttcc gtgacgattt gcaggacggc agtcttgaac
     6901 aattgatgtt gttgccgtta cccttgcccg ccgttgtgct ggcgaaggtg atggcgcact
     6961 ggatggtaac cggtctgccg ttactcatcc tttcgccact ggtagcaatg ctactgggaa
     7021 tggatgttta tggctggcaa gtgatggcgc tgacgctgct gctgggaacg cctacgcttg
     7081 gctttctcgg tgcaccgggt gtggcgttga cagtgggact taagcgcggt ggtgtgctgc
     7141 tcagcatact ggtgttaccg ctgactatcc cattactcat ctttgccacc gccgcgatgg
     7201 acgcggcttc catgcatttg cccgttgacg ggtatctggc aattttaggc gcgttgctgg
     7261 caggcaccgc gacattaagt ccttttgcga cggcggcagc gttacgaatc agcattcaat
     7321 aacgctggat tactgatttt tgttcgagtc tggtatcgaa actatgtgga aaacactgca
     7381 tcaactggcg atcccaccac ggctgtatca aatctgtggc tggtttatac cgtggctggc
     7441 aattgccagt gtggtcgtgc ttaccgtcgg ctggatctgg ggattcggct ttgctccggc
     7501 tgattatcag cagggaaata gctaccgcat tatctacctg catgtgcctg cggcgatctg
     7561 gtcgatgggc atttatgcat caatggcagt ggcagcgttt attggccttg tctggcagat
     7621 gaaaatggcc aacctggcgg tggcggcgat ggcccccatt ggtgccgtgt ttacctttat
     7681 tgccctggtt accggctctg catggggaaa accgatgtgg ggcacctggt gggtatggga
     7741 tgcacgtctg acttctgaac tggtgctgct gtttttgtat gtgggtgtga ttgccctgtg
     7801 gcacgccttc gacgaccgcc gtctggcggg ccgtgcggca ggtatcctgg tgctgattgg
     7861 cgtggtgaat ctgccgatta ttcattactc cgtggagtgg tggaacaccc tgcatcaggg
     7921 atcaacgcgg atgcagcaaa gtatcgatcc ggcgatgcgt tcgccgctgc gctggtcgat
     7981 ttttggcttc ctgctcctgt ctgccacgct gacgctgatg cggatgcgta atttgatttt
     8041 gctgatggaa aaacgccgtc cgtgggtgag tgaactgata ctgaaaagag gccgtaaatg
     8101 acccctgcat ttgcttcctg gaatgaattt ttcgcaatgg gcggttacgc cttttttgtc
     8161 tggctggcgg tggtgatgac cgttattccg ctggtggttt tggtcgtgca ctcggtgatg
     8221 caacatcgcg caattctgcg tggtgtggcg caacagcggg cgcgtgaggc gcgtttacgt
     8281 gatgcgcaac agcaggaggc tgcatgaata ttcgccgtaa aaaccgcttg tggattgcct
     8341 gtgccgtgtt ggcagggctg gcgctgacta tcggtctggt gctatatgcg ctgcgctcga
     8401 atatcgatct cttttatacg ccgggggaaa ttctctacgg caagcgtgaa actcagcaaa
     8461 tgccggaagt cggtcagcgt ctgcgcgttg gcgggatggt gatgccgggt agcgtgcagc
     8521 gcgatcccaa ttcgctaaaa gtaactttca ccatttacga cgctgaaggt tcagtagatg
     8581 tctcttacga aggcattttg ccggatctgt tccgtgaagg gcagggcgtt gtggtgcagg
     8641 gcgaactgga aaaaggcaat catatcctcg caaaagaagt gctggcgaaa cacgacgaaa
     8701 actacacgcc gccagaagtt gagaaagcga tggaagccaa tcaccgtcgc ccggcgagtg
     8761 tttataagga cccagcatca tgatgccgga aattggtaac gggctgctgt gtctggcgct
     8821 aggaattgcg ctgctgctgt ccgtgtatcc gctatggggc gtagcgcgcg gagatgcgcg
     8881 catgatggcg tcttcccgct tgtttgcctg gctgctgttt atgtctgtgg ctggcgcatt
     8941 tctggtactg gtcaatgcct tcgtggtcaa cgacttcacc gtcacctatg ttgccagcaa
     9001 ctccaatacc cagcttccgg tgtggtatcg cgtggcggct acctggggcg cgcatgaagg
     9061 ctcgctcctg ctgtgggtgc tgctgatgag cggctggacc tttgcggtag cgatttttag
     9121 tcagcgtatt ccgctggata ttgtggcccg cgtactggcg ataatgggga tggtcagcgt
     9181 cggctttttg ctgttcattc tctttacctc taacccgttc tcacgcacgt tgccgaactt
     9241 cccgattgaa gggcgcgatc ttaacccgct gttacaggat ccggggctga tcttccatcc
     9301 gcctctgctc tatatggggt acgtggggtt ctcggtggcg tttgcttttg ccattgcttc
     9361 tttgctgagc gggcgtctgg acagcactta tgcgcgtttt actcgcccgt ggacgctggc
     9421 ggcgtggatt ttcctgacgc tcggcattgt gctcggttcc gcatgggcct attacgaact
     9481 cggctggggc ggctggtggt tctgggatcc ggtagaaaac gcctcgttta tgccgtggct
     9541 ggtggggact gcgctgatgc actcactggc ggtcactgaa caacgcgcca gtttcaaagc
     9601 gtggacatta ctgctggcaa tcagtgcctt ctcgttgtgt ctgctgggga ccttcctcgt
     9661 gcgttccggc gtgctggtat cggtacacgc gtttgcgtct gatccggcgc gcggtatgtt
     9721 tatcctcgcc tttatggtgc tggtgattgg cggttcgctg ctgctgtttg ccgcgcgtgg
     9781 acacaaagtt cgctcacgcg taaacaatgc gctgtggtcg cgggaatctt tgctgttagc
     9841 gaacaatgtt ttgctggtcg ctgcgatgct ggtggtgttg ctggggacgc tgctgccgtt
     9901 ggtgcataag caactgggac tgggcagtat ttcgattggc gaaccgttct tcaacaccat
     9961 gtttacctgg ctgatggtgc cgtttgcgct actgcttggt gtcggtcctc tggtgcgctg
    10021 ggggcgggat cgcccgcgta agatccgcaa tttattgatt atcgccttca tctctacgct
    10081 ggtgctgtcg ctgctgttgc cgtggctgtt cgaaagcaaa gttgtggcga tgacggtgct
    10141 cggcctggca atggcctgct ggattgcggt gctggcaatt gcggaagctg cgctacgtat
    10201 ttcacgcggc acgaaaacca ccttcagtta ttgggggatg gtggcggctc accttgggct
    10261 ggcagtgaca attgttggca ttgcctttag ccagaactat agcgttgagc gtgatgtgcg
    10321 catgaagtcc ggcgatagcg tcgatattca tgaatatcgc ttcaccttcc gtgatgtcaa
    10381 agaggtgact ggcccgaact ggcgtggcgg tgtggcgact atcggcgtaa cgcgcgatgg
    10441 caagccggaa acggtgctgt atgcggaaaa acgttattac aacactgccg ggtcgatgat
    10501 gaccgaagcg gcaattgacg gcggcatcac gcgtgacctg tacgccgcgc tcggtgaaga
    10561 gctggaaaac ggcgcgtggg ccgtgcgtct ttactacaaa ccatttgttc gctggatttg
    10621 ggcgggcggg ctgatgatgg cgttgggcgg actgctgtgt ctgtttgatc ctcgctatcg
    10681 taagcgcgtg agtccgcaaa aaactgcgcc ggaggccgta tgaagcgcaa agtattgtta
    10741 attccgttga ttatcttcct ggcgattgcc gcggcgctgc tgtggcagct ggcgcgtaat
    10801 gccgaagggg atgatccgac caatctggaa tcggcgctca ttggcaagcc tgtgccgaag
    10861 tttcgtctcg aatcactgga caatccgggg cagttttatc aggcggatgt gctgactcag
    10921 ggcaaaccag tactgcttaa cgtctgggcg acctggtgtc cgacctgccg tgcggaacat
    10981 caatatctga atcagctttc agcgcagggc attcgcgtgg tcggcatgaa ctataaagac
    11041 gatcgccaga aggcaatcag ctggctgaaa gagctgggca atccttacgc gctaagcctg
    11101 tttgatggcg acggcatgtt agggctggat ctcggtgtct atggcgcgcc agaaaccttc
    11161 cttattgacg gcaacggtat cattcgctat cgccatgcgg gcgatctcaa tcctcgcgtc
    11221 tgggaagaag agatcaagcc gctgtgggag aaatacagta aggaggccgc acaatgaggt
    11281 ttttattggg cgtgctgatg ctgatgatct ccggctcagc actggcgacc atcgacgtgt
    11341 tgcagtttaa agatgaagca caggaacaac agttccgtca gctcactgaa gaactgcgct
    11401 gcccgaaatg ccagaacaac agcattgccg attccaactc gatgattgcc accgacctgc
    11461 gtcagaaagt gtatgaactg atgcaggaag gtaaaagtaa aaaagagatt gtcgattata
    11521 tggtggcgcg ttacggcaac ttcgtcactt acgatccgcc gttaacgccg ctgaccgtgc
    11581 tgctgtgggt gcttccggta gtggctattg gcattggcgg ttgggtcata tacgcccgct
    11641 cgcggcgtcg ggtacgcgta gtgccggaag cgtttcctga acaaagcgtg caggaaggta
    11701 agcgtgccgg atatattgtt tatctgccgg gtattgtggt ggcgttaatt gtggctggcg
    11761 tcagctacta ccagactggc aattatcagc aggtgaaaat ctggcagcag gccacggcac
    11821 aggctccggc gttgctggac agggcgctgg atccgaaaac cgatccgctc aacgaagaag
    11881 agatgtcgcg tcttgcgctg gggatgcgta ctcaactgca aaaaaatccg ggagatatag
    11941 aaggctggat tatgttgggc cgcgttggca tggcgctggg taacgctagt atcgccaccg
    12001 atgcatacgc tactgcgtat cgcctcgatc cgaaaaacag cgatgccgca ctgggttatg
    12061 ctgaagcgtt gacacgttca tctgatccca acgacaaccg cctcggtggt gaactgctgc
    12121 gccagttggt gagaagcgac cacagcaata tccgtgtgtt aagcatgtat gcgtttaatg
    12181 cctttgagca gcagcgattt ggcgaagccg ttgccgcgtg ggagatgatg ttgaaactct
    12241 tacctgccaa cgatactcgc cgtgcggtga ttgaacgtag tatcgcgcag gcgatgcaac
    12301 atttgtcgcc gcaggagagt aaataaaaag aggcccgatg gtgatgccat cgggctagac
    12361 tgttgacaaa gtgcgcttag tttatgccgg atgcggcgtg aacgccttat ccggcctaca
    12421 aaattgtgta aattcaataa attgatcgat ccacgtaggc ctgataagcg tagcgcatca
    12481 ggcagttttg cgtttgtcat aaatctcagc ccgatgataa atccatcggg ctatttttat
    12541 tgtgccccgc gttgttgcag gaacagaatg gtggccgcca cgcgtgagcg gacattgagt
    12601 ttgcgcagca gattgcgaat atgtactttt actgtctgct ctgaaatatt caacaccgag
    12661 gcaatctgtt tatttgacag cccctgtgcc agctcgtgca gaacatccag ctcgcgctcc
    12721 gtcagcacgc tgaagggatc ttcttccgcg ccaaacattt cacgttcacg taagtactga
    12781 ttgacgcgtt cgctaaagac tttgctgcct ttcgctccag cacgaattgc ttccagcaat
    12841 acttccgggt cgctgtcttt caacagataa ccgtctgcgc ctgcgtctat cagcgcaaag
    12901 acatcgctgg aggcatctga tacggtcagg ataataattt gcgcggtaac gccatccctg
    12961 cgcaaggcat tgagggtatc caggccactc atacctttca tattgagatc cagcaagatc
    13021 acgtcgatat ccagtctatt cgccagatcg atcgcgctcg cgccgtcgcc cgcttcggcg
    13081 accacttcaa agccaggatc aagctccagt aactgacgaa caccgcgtcg cataagtgga
    13141 tgatcatcca caatcatcac ctgaaaaggc gttgcttcag gcatagtagt ctcctgaggt
    13201 tttattagaa tcattatgtt tgagcgtagc gttggtaacg ctaccaggcg cgtgaatgtt
    13261 accccatttt atgtatggac atgtagtgaa aactacaacc ttgtcgttcg ctttggcatc
    13321 attttcatta taaagacgga attttgcatc agcttctcat caagaaaaac aactaaaaaa
    13381 tcaatcacaa tacttgtgca tttctttaaa cagtttttaa aaactgactc cgggtatgga
    13441 gctatgggta ttttctgtac ccaatgcttt taactgcaat taatttcata ggatgaaagc
    13501 tcaatgcatc aatctggttc tgtttctctt tgtcgttccg caatatctgt tctggtggct
    13561 acagcgttat attcacccat agcattggca tcaacagttg agtatggtga gacagttgat
    13621 ggtgttgtcc tggaaaaaga tatccagctg gtttatggga ccgccaataa tacgaaaatc
    13681 aatcctggcg ga
//
LOCUS       DAPHBJ010000114        13526 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.115, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000114 DAPHBJ010000000
VERSION     DAPHBJ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.115"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            48..2276
                     /gene="crfC"
                     /locus_tag="RHZ76_002977"
     CDS             48..2276
                     /gene="crfC"
                     /locus_tag="RHZ76_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HDV2813580.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQIEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALKVQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            2273..3151
                     /locus_tag="RHZ76_002978"
     CDS             2273..3151
                     /locus_tag="RHZ76_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HDV2813581.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            3415..4917
                     /gene="proP"
                     /locus_tag="RHZ76_002979"
     CDS             3415..4917
                     /gene="proP"
                     /locus_tag="RHZ76_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HDV2813582.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            5029..5118
                     /gene="pmrR"
                     /locus_tag="RHZ76_002980"
     CDS             5029..5118
                     /gene="pmrR"
                     /locus_tag="RHZ76_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HDV2813583.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(5094..6194)
                     /gene="pmrB"
                     /locus_tag="RHZ76_002981"
     CDS             complement(5094..6194)
                     /gene="pmrB"
                     /locus_tag="RHZ76_002981"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HDV2813584.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDDGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(6195..6863)
                     /gene="basR"
                     /locus_tag="RHZ76_002982"
     CDS             complement(6195..6863)
                     /gene="basR"
                     /locus_tag="RHZ76_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HDV2813585.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6860..8503)
                     /gene="eptA"
                     /locus_tag="RHZ76_002983"
     CDS             complement(6860..8503)
                     /gene="eptA"
                     /locus_tag="RHZ76_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HDV2813586.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(8607..9944)
                     /gene="adiC"
                     /locus_tag="RHZ76_002984"
     CDS             complement(8607..9944)
                     /gene="adiC"
                     /locus_tag="RHZ76_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HDV2813587.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10081..10842)
                     /gene="adiY"
                     /locus_tag="RHZ76_002985"
     CDS             complement(10081..10842)
                     /gene="adiY"
                     /locus_tag="RHZ76_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HDV2813588.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFVSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11167..13434)
                     /gene="adiA"
                     /locus_tag="RHZ76_002986"
     CDS             complement(11167..13434)
                     /gene="adiA"
                     /locus_tag="RHZ76_002986"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HDV2813589.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEVTPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         3260 a         3305 c         3547 g         3414 t
ORIGIN      
        1 atctggctaa ataaaataac aaaatttgct ttaaggaaga attttctatg tacacacaga
       61 ccctgtatga gttaagtcag gaggctgaac gcctgttaca gctttctcgc caacagttgc
      121 agttactgga aaaaatgcct ctctctgtac ccggagacga cgcgccacaa ctggctttac
      181 cctggagtca gcctaatatc gccgaacgtc acgcgatgct gaataatgag ttgcgtaaaa
      241 tttcccgact ggaaatggtg cttgcaattg tcggtaccat gaaagcaggg aaatcaacca
      301 ctattaatgc cattgttggt acggaggttc tgcctaatcg taatcgccca atgactgcgc
      361 tgccgacgct tattcgccat acgcccgggc aaaaggaacc ggtactgcat ttttcacatg
      421 tcgcgccaat cgattgttta attcaacaat tacaacagcg cctgcgtgat tgcgatatta
      481 agcatctgac cgatgtgctg gaaatagata aagatatgcg tgcgcttatg cagcggatcg
      541 aaaatggcgt cgctttcgaa aaatattatc tgggtgccca gcctattttt cattgtctga
      601 aaagtttgaa tgatttagtg cgactggcga aggcgctgga cgtcgatttt cctttttctg
      661 cttacgccgc cattgagcat attcccgtga ttgaagtgga gtttgtccat ctagcggggc
      721 tggagagtta tcccggtcag ttgacgttac tggatacccc cgggccaaat gaagccgggc
      781 aaccgcatct gcaaaaaatg cttaaccagc agctggcacg cgcctcggcg gtactggcgg
      841 tgctggatta tacgcaactg aaatcgatct ccgatgaaga ggtccgtgag gcgattttgg
      901 cggtggggca atcggtgccg ctgtacgtgc tggtcaataa gttcgatcaa caggatcgta
      961 acagtgacga cgccgaccag gtgcgggcac tgatttccgg gacgctgatg aaaggctgta
     1021 ttacgccaca gcagatattt ccggtgtcgt cgatgtgggg ctacctggcg aatcgggcgc
     1081 gttatgagtt agccaacaac ggtaagttac caccgccaga gcaacaacgc tgggtggaag
     1141 attttgccca tgccgcgctt ggcaggcgct ggcgtcatgc cgatctggcg gacctcgaac
     1201 atattcgtca tgctgccgat cagttgtggg aagattcgct gttcgcccag ccaattcagg
     1261 cgttgcttca tgccgcttac gctaacgcct cgttgtatgc tctgcgatct gccgcgcata
     1321 aactgttgaa ttacgcgcag caggcgcggg aatacctgga ttttcgtgcg cacgggttaa
     1381 acgtcgcttg tgaacaattg cggcaaaata tccaccagat cgaagaaagt ttgcagctat
     1441 tgcaactcaa tcaggcgcag gtgagcggcg agattaaaca tgaaatcgag ctggccctga
     1501 cctccgccaa ccactttctg cgtcaacagc aagatgcgct gaaggtgcag ttagccgcct
     1561 tgtttcagga tgattcggag ccgttaagcg agattcgtac ccgctgtgag acactgttac
     1621 agacggcgca gaacaccatc agtcgcgact ttacgctgcg ttttgccgag cttgaatcca
     1681 ccctttgccg ggtgttaacc gatgttattc gccccattga gcaacaagtc aaaatggaat
     1741 tgagcgagtc agggtttcgt cctgggtttc attttcctgt ttttcacggc gtagttcccc
     1801 acttcaacac tcgccagctg ttcagtgaag tcatttcgcg ccaggaagca acggacgagc
     1861 agagcacgcg tttaggcgtt gtgcgtgaga ctttttcgcg ctggttgaat cagcccgact
     1921 ggggacgggg aaatgagaaa tcaccgacag aaacggttga ttacagtgtg ttgcaacgag
     1981 cattaagcgc agaagtcgat ctctattgcc aacaaatggc taaagttctg gcagagcagg
     2041 tcgatgaatc tgttacggca ggcatgaata cttttttcgc tgagttcgct tcatgtttga
     2101 cggaattaca gacgcgttta cgcgaaagtc tggctctgcg tcaacaaaat gaatcggtgg
     2161 tcaggctgat gcagcagcaa ttgcagcaga ctgtgatgac tcacggctgg atttacaccg
     2221 acgcccagct gttacgcgat gatattcaaa cacttttcac ggcagaacga tattgaccaa
     2281 gacgttactt gacggccccg gtcgcgtgct ggagtcggtt tatccccgct ttttagtgga
     2341 tctggcgcag ggtgatgatg cccgccttcc acaagcccat cagcagcagt ttcgtgaacg
     2401 actgatgcag gaacttcttt cgcgtgtgca gcttcagaca tggacgaacg gcggcatgtt
     2461 aaatgcgccg cttagcctgc gtctgacatt ggtggaaaaa ctggcgtcga tgctggatcc
     2521 cggtcatctg gcactgacgc agatcgcgca gcatctggcg ctgctgcaaa aaatggatca
     2581 ccgccagcac tctgctttcc cggagctccc ccagcaaatt gccgccttgt atgagtggtt
     2641 ttcagcccgt tgtcgctgga aggaaaaggc gttaacgcaa cgaggcctac tggtgcaggc
     2701 aggtgatcag agcgagcaaa tttttacccg ctggcgtgct ggggcgtata acgcctggtc
     2761 gttgcctggg cgctgtttta tcgttctcga agagttgcgt tggggggcat ttggcgatgc
     2821 ctgtcgtctg ggaagcccgc aagcggtggc gttgttgctg ggtgatttgc gcgtgaaagc
     2881 gacacaacat ctggcagaga gtatcaatgc ggccccgacc acgcgtcact attaccatca
     2941 gtggtttgcc tcttcgaccg ttccgacggg cggggatcat gctgattttt taagttggct
     3001 gggaaagtgg accacggcag ataaacaacc cgtttgctgg tcagtgaccc aacgttggca
     3061 aactgtcgcg ctggggatgc cacgactctg ttcagcgcag cgtttggtgg gggcaatggt
     3121 cgaggaaatc ttctctgtaa atttggcgta aataatcagt tacatcaatg agtcctaaac
     3181 gaaatccatg tgtgaagttg atcacaaatt taaacactgg tagggtaaaa aggtcattaa
     3241 ctgcccaatt caggcgtcaa ctggtttgat tgtacattcc ttaaccggag ggtgtaagca
     3301 aacccgctac gcttgttaca gagattgcat cctgcaattc ccgctcccct tttgcggccg
     3361 tcgcgctgat ttttctggcg tttgcggaaa tgggccaact ctgcgaggaa agctatgctg
     3421 aaaaggaaaa aagtaaaacc gattaccctt cgtgatgtca ccattattga tgacggtaaa
     3481 ctgcgtaaag ccattaccgc agcatcactg ggtaatgcaa tggaatggtt cgattttggt
     3541 gtttatggtt ttgttgctta cgcattaggt aaagtttttt tcccgggggc tgaccccagc
     3601 gtgcagatgg ttgctgcact tgccactttc tccgttccct ttctgattcg accgcttggc
     3661 ggactcttct ttggtatgtt gggcgataaa tatggtcgcc agaagatcct cgctatcact
     3721 attgtgatta tgtcgatcag tacgttctgt attggcttaa taccgtccta cgacacgatt
     3781 ggtatttggg caccgattct gctgttgatc tgtaagatgg cacaaggttt ctcggtcggc
     3841 ggtgaatata ccggggcgtc gatatttgtt gcggaatact cccctgaccg taaacgtggc
     3901 tttatgggca gctggctgga cttcggttct attgccgggt ttgtgctggg tgcgggcgtg
     3961 gtggtgttaa tttcgaccat tgtcggcgaa gcgaacttcc tcgactgggg ctggcgtatt
     4021 ccgttcttta ttgctctgcc gttagggatt atcgggcttt acctgcgcca tgcgctggaa
     4081 gaaactccgg cgttccagca gcatgttgat aaactggaac agggcgaccg cgaaggtttg
     4141 caggatggcc cgaaagtctc gtttaaagag attgccacta aatactggcg cagcctgttg
     4201 acatgtattg gtctggtaat tgccaccaac gtgacttact acatgttgct gacctatatg
     4261 ccgagttatt tgtcgcataa cttgcattac tccgaagacc acggggtgct gattattatc
     4321 gccattatga tcggtatgct gtttgtccag ccggtgatgg gcttgctgag tgaccgtttt
     4381 ggccgtcgtc cgtttgtgct acttggtagt gttgccctgt ttgtgttggc gatcccggcg
     4441 tttattctga ttaacagtaa cgtcatcggc ctgatttttg ccgggttact gatgctggcg
     4501 gtgatcctta actgctttac gggcgttatg gcttctacct tgccagcgat gttcccgacg
     4561 catatccgtt acagcgcgct ggcggcggca tttaatattt cggtgctggt tgccggtctg
     4621 acgccaacac tggcggcctg gctggtcgaa agctcgcaga atctgatgat gcctgcctat
     4681 tacctgatgg tagtggcggt gattggttta atcaccggcg taaccatgaa agagacggca
     4741 aatcgtccgt tgaaaggtgc gacaccggcg gcgtcagata tacaggaagc gaaggaaatt
     4801 ctcgtcgagc attacgataa tatcgagcag aaaatcgata atattgacca cgagattgcc
     4861 gatttgcagg cgaaacgtac ccgcctggtg cagcaacatc cgcgaattga tgaataagct
     4921 gaaacggatg gcctgatgtg acgctgtctt atcaggccaa ttgaactctt aaggttcact
     4981 taatctctga cgcgcatact ctcctccagg ttaacggagg agagtgcaat gaaaaaccgt
     5041 gtttatgaaa gtttaactac cgtgttcagc gtgctggtgg tcagcagctt tctttatatc
     5101 tggtttgcca cgtactgatc tttcttcagc cgtacccagg cccgcgtgcc ggaagtctct
     5161 tgccggtttt gcaggaaaaa ctgcccgtga tgcaactgtg taatgcggct gacaatactt
     5221 aaccccagac caatcccgcc ataacggctg tccatacgta caaacgcttt actcaactcc
     5281 ccgcatttac tctcatcaat acctggtcct tcatcttcaa ctgccatgac cgctccgtca
     5341 tcttcttgca gcttaatcat aatgttgctg ccttgcgggc tgtaacgatg ggcgttttct
     5401 accaggtttc gcaataacat ccgcagcagg gttgcatcac cctgaacggt gatgtcggcg
     5461 gcgctctctg gcaatagcag ggtttgctgt cgctggtcga gcatggtact gagttcgtca
     5521 tacgagggga gaatgacatc ttccagcagt tttacatgtt gataattacc ggaagaaaat
     5581 gactgtccgg cacgcgccag ttgcagcagc tgggagacgc tctccatcat ctgatcaagc
     5641 cgtgccacta acggtgctac atcaatgtga tgcgttttcg ccagcagttc cagatgcaaa
     5701 cgcacccccg ccagtggcgt tcgcagttcg tgcgcgacgt cagcggtaaa caacctttcg
     5761 ttatccagcg tgctggtcag gcgactgacc agatcgttta acgccgaaac caccgcttcg
     5821 atttcgaggg tggcgctgtg aatggcaatg ggcgttaagt tgtcggcggt gcgcgcttcc
     5881 agctcttttt gcagctccgc cagcgggcgg gtgatgcggc gtaccgcctg atagcagata
     5941 aatagcgtca ggctgaccat aaagacgccg gggacaatca ggctggcgac cgcctcgcgg
     6001 atctcacgca tgatgtggcg atcgttgttg cgattgtcgc gtagcgcctg ctcaaacagc
     6061 tgaatctgct cggtactttc atgccatagc cagaagacgc tgatcagctc aaacaccaac
     6121 aaaatggccc cgatggtcaa tatcagccgt tggcgcagcg atattggtcg gcgcagaaaa
     6181 cgcatcagat tcaattagtt ttcctcattc gcgaccagca tatagccaaa gccgcgcacg
     6241 gtgcggatac gggctttgcc cactttgtcg cgcagattgt ggatatgcac ttccagggtg
     6301 ttggtcgagg gttcattgtc ccagttatag atgtcgttgt agagaatttc ccgatgcacc
     6361 ggactgcctg ctttgagcat taaccgtgac agcagagcat attctttggg cgtcagaatc
     6421 aactcttcac cgcccatcca tacctgacgg cgacccatat tcagcgtcag attgccaaca
     6481 atcagctcac tttcgccctg attattatgg cgtcgtagca gggctcggat acgggcgtgt
     6541 aactcttcca gcgcaaaggg cttcaccaga tagtcatcgg caccgacatc cagcccggcg
     6601 attttgtcgg tcagcgtatc gcgagcggtg aggatcagta ccggcagggt gtattttttc
     6661 tgccggatac gggcgagaaa atgcagtcca tcttcgtcgg gtaaccctaa atccagtacc
     6721 accaggctgt aatgaccggc ctcaaggctt tgttccgcca tccgcgcggt tgtcacgcca
     6781 tcgcacgcgt agccttcggt ttgcgccgcc agaatcagtc cctgcaataa cagcgtatcg
     6841 tcttcaacaa tcagaatttt catttactca ctctcctgca agtttgcaga atatcatccg
     6901 cagcctggta atacttcgtc tcaacgccag ttaatcccaa tagcgtggag aataaattgt
     6961 cttgtgaata gtgttgcgtt tgcgcctgtt tttgcaggca gttctggtca acctgatacc
     7021 gtttttgata atcctccgac agccacagca gcattggcac ctgtttttgg ctatccgggg
     7081 cgatggcata aggcagaccg tgcagataga tgccattttc acctaacgat tcaccgtggt
     7141 cagaaagata aaccaggctg gtggtaaatt tatcctgatg ttctttaagc agattaatcg
     7201 ctttatcaac aatatagtcg acgtaaacca gcgtgttgtc gtaagtgttc accagttgct
     7261 ctttggtaca ggtctggatc tcattggtgt cgcaggttgg ggtaaatttc ctgaactgag
     7321 gcggatagcg gttgtaatag gtcggaccgt ggctgccgat ggtgtgtaag acaatcacgc
     7381 catcaccttg caggttattg atgtactctt caagcccgtg gaacagcact tcgtcatagc
     7441 attcgccgtt gatgcactga tcaggtagat tcagcgcggt gacgttctgg tgaggcacgc
     7501 ggtcgcaggc acctttacag ccgccatcgt tgtcattcca cagcacgttg atgcccgctc
     7561 gctgaatgat atccagcacg ccttcctggt gctgtgccag ctcttctttg tagtgctcac
     7621 gcggcatatc cgagaacatg cacggtactg aaactgccgt tgccgtgccg caagatgcgg
     7681 tattagggaa atagaccacg ttatctttcg ccagccgcgg gttagtttca cgcgggtagc
     7741 cgttgaggga gaagttctcc gcccgcgagg tttcgccgac aatcaggata gtcaaatttt
     7801 tacgtttttc gttctgcatt aacgggttgc ggtgcgcgtc ttcaccaatt cgcaccagcg
     7861 gcagatttgc cagtcgctga tgggagtacc atgaccagct ggcaacaatg ctgttagagg
     7921 ggcttaagga tttcaccagc tctttgttat tgcggaacaa cgaggcgtag tctttataaa
     7981 acagtgcggc gaccagcaaa atcagtagta cagaaaccag aatattggct ccacggaaaa
     8041 gaacactgcg cagacgcgag gtggcaggtt tgatttttat ccagcaggca atcagcgcag
     8101 caagcacgcc gctgaatccc agcgttaata acatttgcgg tgtcatcagc gcataacttt
     8161 ctgccggagt ggtatcaata atattggcaa tcatcgagcg gtcgatgacg atgccgtaag
     8221 tcattatgaa atattgtgca gccgcgccaa ccagaataaa caggcaggcc agtggtcgat
     8281 taagccataa gaaagagctt agtgtcagga caatattaat cacgctgaaa gcgacgaccg
     8341 gcatcgacaa gaaaaccagt acgttatgca gcgaatccag cggcagcgcc tgcaacacct
     8401 gtttaaaaaa ggcaatattc aggcagatag agatataaaa agcggccaac aatagccagg
     8461 cgagtaaatt caaagagggt ctttttagta ggcgcttcaa catcacggtg tttccatcga
     8521 acaaagtgcg catatgctcg caaagcaaaa ttaagccaac cttaagttct taaggttggc
     8581 ttttatgttt gctggattaa ggggaattaa tctttgctta ttggtgcatc taagggatac
     8641 gggtttttat gtagccggtt gtaattcagc gcatacatgg cggtgatgac catcagggtg
     8701 acaaatgacc acataacctc tttcgctccg gaccccacca cggcccagat gcagtagagg
     8761 aaggcaatgg tagtaactgc cagatatgcc gggcgtgctt taccaaagtg accgtgtccg
     8821 agcagcagta acgccgcaca ggtgtaaaga tatggcacca gtgtaaagat gaccgagacg
     8881 gaagaaacca gaccgaactc tttggtcgcg tttggtgaaa tgctgctgag ctggaagatg
     8941 gtcatcaaaa taccgacgat aatcagcccc gccactggcg tacccgcttt atttacacgg
     9001 gcaaaaatcg gtgggaacag tccgtcatcg gcagcggctt tcgccgtttg acccgccagc
     9061 aacgtccagc cgcccagtga acctaagcaa cccgcagctg cgcagaagga aacaatggcc
     9121 ccggcggtgt cacccaacgc catccgtgcg gcatcaccga atggcgaagc agaaacgcgc
     9181 agtgcggcat tagggatcat ccccataatc gcggtggtag aaagtacata gcaaacggcg
     9241 gcaatcaata ccccaccaat ggtggcgata gggacattgc gtttcgggtt tttcaccaca
     9301 cctgcggcaa cggaggcact ttccacaccg atgaacgacc acagcgtaac gttaagggta
     9361 ctttgaattg caccgaaggt gcccaggccg ctgacgttcc atgccgccat ataggtttca
     9421 ccacggaacc agaaccagcc aaatacggca atcccgacga tggggatcag cgccagcacg
     9481 gtggcaactg cctgcacacg ggtgatcatt ttcggaccga caatgttcag caggacgaag
     9541 atccacagca ccacgacgca ggtgatggtt aataccaatg gatctttcag aatcgggaag
     9601 aagtaactta aatatcctac gccaatgacc accatggcga tattgccgat ccagcaggcc
     9661 agccagtaga ggacgttggt ttgataaccg agaaacgggc caaagcagcg gcgggcgtaa
     9721 gcgtaagaac caccaggact tgggtcgagg aacgacattt tggcgtatac catcgagagc
     9781 cccagtgcac cgataatcgt caccaaccat ccataaatgg caatcccgcc agtagaggcc
     9841 aggtttgcag gtaacagaaa aacacctgac cccataatat tccccgacac catcagggtg
     9901 acggggatta agcccacttt gtgagcatca gcatccgaag acataattaa actcctgcga
     9961 aggcgagctt cgtgacaata aattacgtca tatcatacgc ctgcattcga tagttgaatt
    10021 attatcgacc gggttatcgc tgatgccgtt attaatatag ctcttcgtta aggttaaaaa
    10081 tcaggcgaca gtgcgttctc tgtgctgact aacataatgc agcggcgtca taccgtagaa
    10141 gtctttaaag acagaaataa agtacgacgt actgttgtag ccgcaggact gtgatacctg
    10201 agagatattt ttaccgtcca tcattaattc atttacggca taacgcatgc ggcaggtggt
    10261 gattatttgg ctataactgg tgttttcgct tttcaacttt ttctttaaca gacttgggct
    10321 aagacataaa caactggcta ccatactaag attccagtct ttgtgaatat cgctttcaat
    10381 aatttgataa acgctgtcac ttacacaatt acgtaacata tacatcatta atgaaacgaa
    10441 ttttttactg tcgagaaaac gggatagcac cgtgaataat aatgcgcgtg ttcgttcctt
    10501 ttctgactca gtttctgcgg gcatcatgct gtgttgcgcg gcaacgcgaa agacgtctgg
    10561 cgtcaggcat ggcagggtga gtatgggcgt agcgctacgt tgccatactg gtatttgcga
    10621 gagatcttta ttcagaaacc ggaggtaatc tttgatgatg tcgtgactaa tatgagcaac
    10681 caaagtgttg ttaagagagg agacgtcgat aatattattt tcacaattta ataacgccat
    10741 atggttagct ttaaggctaa taggttcttt cccattcacc cttatccaga catctttttc
    10801 agtcaataaa acaatacaag gttggtcgct gcaaatcctc atacatgtac tcctgagtgc
    10861 gaataaaaga ccagttctcg ctaaagcaaa gcgataccgg gaaatggtta tataattgta
    10921 tgatgaatat aaacatgcat aaaaataacg agttttacta atagtacaaa tttttatagt
    10981 aatttctcat gtacatattt ttttacagga taaataacag gcaaaaaaaa gcgcggggtg
    11041 gggccgcgca aaaagtatga catttttgtt tgcagcaatt tttatgaaaa cgttgccata
    11101 ctcaaaaggc ctaaggccag gcggcgtaat gttatttaaa caattacgcc ttcagcggaa
    11161 tagtggttac gctttcacgc acataacgtg gtaaataccg tcaataattt cagtcccttc
    11221 agtttcgtgt tcaaatccag ggaaatggtg gtcccaggat tgcagcgagc gtaaataact
    11281 tacttgcgga ctgtttttat cgccgaagtt ttcaccagac agcagcatcg ggattcctgg
    11341 cggatacggg ataactgagt ttgccgcgat gcgtcctggc agattttcaa tggataccag
    11401 ttcgacattg ttgtcgacaa tcgcgttgta cgcttcacgc ggggtgactt ccgccaccgg
    11461 caggccggaa taggcttcgt tcaaccgtgc gccagggttg ttttctttca gccaggcaaa
    11521 catggtgtca cccagatcgt gaatccccat gttcgcgtaa gtgtcaggat attgttcaac
    11581 aagttccggc atcacctgcg ccagcggtgt gttggcgtca tagtggcgtt tgaaggagca
    11641 aagggtgtta accagagttc cccatttccc acgggttacg cccatagaga acaggaacat
    11701 aatttggaag tcagtggtgc gggtaggtac aatgccgtgg cgaccaagcc aggcagtgac
    11761 cagcgccgcc ggaacaccgg tttcttccag ttcaccatct tcacccattc ccggagcaag
    11821 gatgctgact ttaatcgggt cgagcatact ccagttatcc ggaatatctt tgaagccgtg
    11881 ccagctttcg cccggatgca ttacccagca gtcctgaacg gtggtcagca gtttggttgg
    11941 tgcgtcagca aagtcatagg ttttgccggt ttgtgggtcg gtgacgactt ctttgttcca
    12001 cggtttgaag aaccagctac cgtcagcggt gaactcttta tatagccgcg ccatcgcctg
    12061 acggaaatca accgcttcgt caatcacttc ctgtgtcagt gacaggccgc tgttgccgtc
    12121 catcatcgac accgccacgt cgttggatgc gcagatggca tacagcgggg aggtggtggc
    12181 atgcatcatg taggcctggt tgaagcggga gaagttaatc gccccacgac cttcacgtac
    12241 atgaatataa gaagcctgtg acagcgcatt cagcagtttg tgggtggagt gggtggcgaa
    12301 aacggtagga ccgttgtgat cgccaggttc gccgcgcatg gcatagtgat cggcatagat
    12361 cgggttgaaa cgtgcatagc cgtaccaggc ttcgtcaaag tgcagacgat cggaggtttt
    12421 ttccagcaga tcctgcgctt ctttagcgtt ataacacacg ccgtcatagg tgcagttggt
    12481 caccacgcag taagacggtt tttgcccggc tttgtctttg gtcagcgggc tttcactgat
    12541 tttcttctgc aaggtttcag gttgcatttc ctgcggatag attggcccga taatgccgta
    12601 gcggttgcgg cttggcacca tatagaccgg tttcgcgcct gtcagcatca aaccttgttc
    12661 gatggattta tggcagttac ggtcaacgac cacgacatcg ttatcggtca tgcaagcctg
    12721 catgatggtg cggttagagc cggaagtacc gacgactacc gaccaggagc gatcggcacc
    12781 aaatacgcgt gcggcatatt tttcgctttc gccaaatgcg ccagtatggt caagcaaaga
    12841 accgagggaa gttcgttcga tgcccatgtc ggtgcggaac agattttcac catagtagtc
    12901 atggtagaaa cgtccggcgg gtgttttggt aaaaccaacg ccgccctggt ggcctggcgc
    12961 tgcccaggaa tattcatgga tgtcactata tttcatcagc gcgctgaaca gtggcggcaa
    13021 cagctgctgg cggtagcggg tcatcgcggc aacggcgcgt ccggcgataa agtcggcggt
    13081 atcttccaga atccaggcga attcatcgac aagctccagc aggtcgcgat ccattgcggc
    13141 gagggctttt tcccgatcgc ccaacaggaa gaccggcacg ttttgttggc gctcatgaag
    13201 cttaccgatc aattgtctga cgttttgatg ttcgtccgga tgttccattt gatagctgaa
    13261 catcaggcag tcaatggctt cgtttgaaga gagaatggca aaaccatcat caaaggaggt
    13321 ggatttaatc acggtaacat tttgctggct taaagcatct gccagacgct caacggcgtt
    13381 accgacccag gtgtcttgat ggagaaactc gctttcaaca attaatactt tcatcattgc
    13441 ttacccggtt atgaaggaat cttcgttgcg ggcaagtatc ttccggcctt ttcgggctgt
    13501 aaagcgcgtg aaaaacaaac attttt
//
LOCUS       DAPHBJ010000115        13471 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.116, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000115 DAPHBJ010000000
VERSION     DAPHBJ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.116"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            171..1988
                     /gene="pcoA"
                     /locus_tag="RHZ76_002987"
     CDS             171..1988
                     /gene="pcoA"
                     /locus_tag="RHZ76_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase PcoA"
                     /protein_id="HDV2813590.1"
                     /translation="MLLKTSRRTFLKGLTLSGVAGSLGVWSFNARSSLSLPVAASLQG
                     TQFDLTIGETAVNITGSERQAKTINGGLPGPVLRWKEGDTITLKVKNRLNEQTSIHWH
                     GIILPANMDGVPGLSFMGIEPDDTYVYTFKVKQNGTYWYHSHSGLQEQEGVYGAIIID
                     AREPEPFAYDREHVVMLSDWTDENPHSLLKKLKKQSDYYNFNKPTVGSFFRDVNTRGL
                     SATIADRKMWAEMKMNPTDLADVSGYTYTYLMNGQAPLKNWTGLFRPGEKIRLRFING
                     SAMTYFDIRIPGLKMTVVAADGQYVNPVTVDEFRIAVAETYDVIVEPQGEAYTIFAQS
                     MDRTGYARGTLATREGLSAAVPPLDPRPLLTMEDMGMGGMGHDMAGMDHSQMGGMDNS
                     GEMMSMDGADLPDSGTSSAPMDHSSMAGMDHSRMAGMPGMQSHPASETDNPLVDMQAM
                     SVSPKLNDPGIGLRNNGRKVLTYADLKSRFEDPDGREPGRTIELHLTGHMEKFAWSFN
                     GIKFSDAAPVLLKYGERLRITLINDTMMTHPIHLHGMWSDLEDENGNFMVRKHTIDVP
                     PGTKRSYRVTADALGRWAYHCHLLYHMEMGMFREVRVEE"
     gene            1994..2884
                     /gene="pcoB"
                     /locus_tag="RHZ76_002988"
     CDS             1994..2884
                     /gene="pcoB"
                     /locus_tag="RHZ76_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance outer membrane transporter
                     PcoB"
                     /protein_id="HDV2813591.1"
                     /translation="MKRNLKAIPVLVAGLFTSQLSIAAGSVSADPHAGHDMSAMQMPA
                     DENFTEMTSMEPIVTESRTPIPPVTDADRKAAFGNLQGHAIHDSAINYLVLLDQLEWQ
                     RSDNTNNFSWSVNSWIGGDTDRIWLKSEGERSNGETEAAEAQLLWGHAVGPWWDLVAG
                     VRQDFRPASARTWAAVGFQGLALYNFESEITGFVSNGGKAALRLGGEYDVLLTNRLIL
                     QPSYEVNFYSQDDESRGRGRGLTDTELGLRLRYEIRREFAPYIGVSWNQLYGKTSDMA
                     KREGEKDHQVVFLAGARIWF"
     gene            2924..3304
                     /gene="pcoC"
                     /locus_tag="RHZ76_002989"
     CDS             2924..3304
                     /gene="pcoC"
                     /locus_tag="RHZ76_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance system metallochaperone PcoC"
                     /protein_id="HDV2813592.1"
                     /translation="MSILNKAILTGGLVMGVAFSAMAHPELKSSVPQADSAVAAPEKI
                     QLNFSENLTVKFSGAKLTMTGMKGMSSHSPMPVAAKVAPGADPKSMVIIPREPLPAGT
                     YRVDWRAVSSDTHPITGNYTFTVK"
     gene            3309..4238
                     /gene="pcoD"
                     /locus_tag="RHZ76_002990"
     CDS             3309..4238
                     /gene="pcoD"
                     /locus_tag="RHZ76_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance inner membrane protein PcoD"
                     /protein_id="HDV2813593.1"
                     /translation="MNDLIMIVIRFLLYLDLMVIFGLPFFQIYGISGVRHETYNLTNF
                     RSFITFAVVTGIILTGINMLLVSNAMSGVTDLRELSIHVIEMVIEETDVGISWIVRLC
                     ALFTTLGALFLYTNKRVLSCLLMTMSGGVALATLAWGGHAVMHDGLHYYLHLLSDLTH
                     LGAAGAWTGALVAFAILLMRRNEHNAQSVIVISDSLAKFATAGTVIVVALILSALVNY
                     LYIAEGNLTPLFNSSWGRILLAKTALFVLMLLLAAANRFHLGPRLEVMVREGNYDRSV
                     ALMRNSILTEFVVAIIILGAVAWLGMLAPSQIS"
     gene            4293..4973
                     /gene="pcoR"
                     /locus_tag="RHZ76_002991"
     CDS             4293..4973
                     /gene="pcoR"
                     /locus_tag="RHZ76_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     PcoR"
                     /protein_id="HDV2813594.1"
                     /translation="MQRILIVEDEQKTGRYLQQGLVEEGYQADLFNNGRDGLGAASKG
                     QYDLIILDVMLPFLDGWQIISALRESGHEEPVLFLTAKDNVRDKVKGLELGADDYLIK
                     PFDFTELVARVRTLLRRARSQAATVCTIADMTVDMVRRTVIRSGKKIHLTGKEYVLLE
                     LLLQRTGEVLPRSLISSLVWNMNFDSDTNVIDVAVRRLRSKIDDDFEPKLIHTVRGAG
                     YVLEIREE"
     gene            4970..6370
                     /gene="pcoS"
                     /locus_tag="RHZ76_002992"
     CDS             4970..6370
                     /gene="pcoS"
                     /locus_tag="RHZ76_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance membrane spanning protein
                     PcoS"
                     /protein_id="HDV2813595.1"
                     /translation="MRFKISLTTRLSLIFSAVMLTVWWLSSFILISTLNGYFDNQDRD
                     FLTGKLQLTEEFLKTETFRNKTDIKSLSEKINDAMVGHNGLFISIKNMENEKIVELYA
                     KNSVVPAVLLNKSGDILDYMIQTEENNTVYRSISRRVAVTPEQGKSKHVIITVATDTG
                     YHTLFMDKLSTWLFWFNIGLVFISVFLGWLTTRIGLKPLREMTSLASSMTVHSLDQRL
                     NPDLAPPEISETMQEFNNMFDRLEGAFRKLSDFSSDIAHELRTPVSNLMMQTQFALAK
                     ERDVSHYREILFANLEELKRLSRMTSDMLFLARSEHGLLRLDKHDVDLAAELNELREL
                     FEPLADETGKTITVEGEGVVAGDSDMLRRAFSNLLSNAIKYSPDNTCTAIHLERDSDC
                     VNVMITNTMSGQVPANLERLFDRFYRADSSRFYNTEGAGLGLSITRSIIHAHGGELSA
                     EQQGREIVFSVRLLMD"
     gene            6586..7020
                     /locus_tag="RHZ76_002993"
     CDS             6586..7020
                     /locus_tag="RHZ76_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HDV2813596.1"
                     /translation="MKKILVSFIAMMAVASSAWAAETMNMHDQVNNAQAPAHQMQSSA
                     EKSAVQGESMKMMDMSGHDQAAMSHEMMQNGNSAAHQDMAEMHKKMMKSKPENTQETA
                     KPFSEMNEHEKAAVVHEKANNGLSSVIHQQQAEKHRSQITQN"
     gene            complement(7220..8812)
                     /locus_tag="RHZ76_002994"
                     /pseudo
     CDS             complement(7220..8812)
                     /locus_tag="RHZ76_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063945310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(8843..9193)
                     /gene="tnpB"
                     /locus_tag="RHZ76_002995"
     CDS             complement(8843..9193)
                     /gene="tnpB"
                     /locus_tag="RHZ76_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV2813597.1"
                     /translation="MIPLPSGTRIWLVAGVTDMRKSFNGLGELVQHVLDDNPFSGHLF
                     IFRGRKGDTVRILWADADGLCLFTKRLEEGQFVWPAVRDGKIAITRSQLAMLLDKLDW
                     RKPKTARLNSLTML"
     gene            complement(9190..9522)
                     /locus_tag="RHZ76_002996"
                     /pseudo
     CDS             complement(9190..9522)
                     /locus_tag="RHZ76_002996"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            9592..10451
                     /locus_tag="RHZ76_002997"
                     /pseudo
     CDS             9592..10451
                     /locus_tag="RHZ76_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            10733..11809
                     /locus_tag="RHZ76_002998"
     CDS             10733..11809
                     /locus_tag="RHZ76_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDV2813598.1"
                     /translation="MVDKVTEAAVVGGVDTHKDLHVAAVVDQNNKVLGTQYFSTTRQG
                     YRQMLAWMTSFGTLKRIGVECTGTYGSGLLRYFQNAGLEVLEVTAPDRMERRKRGKSD
                     TIDAECAAHAAFSGIRTVTPKTRDGMIESLRVLKTCRKTAISARRVALQIIHSNIISA
                     PDELREQLRNMTRMQLIRTLGSWRPDASEYRNVTNVYRISLKSLARRYLELHDEIADL
                     DVMIAAIVDELAPELIKRNAIGYESASQLLITAGDNPQRLRSESGFAALCGVSPVPVS
                     SGKTNRYRLNRGGDRAANSALHIIAIGRLRTDAKTKEYVARRVAEGHTKMEAIRCLKR
                     YISREVYTLLRNQNRRINSIPITA"
     gene            12130..13200
                     /locus_tag="RHZ76_002999"
     CDS             12130..13200
                     /locus_tag="RHZ76_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDV2813599.1"
                     /translation="MQNNFQSPESVILGVDTHLDIHVGAVISQAGKLLGTRIIQTNQI
                     GYQELFDWAKSFGVLERAGIEGTGTYGAALTRFLIKNSIYVVEVNRPDRSKRRLEGKS
                     DPLDAENAARTVLSGSSKAIPKMQSGACEALRIISVARRSAVKARTQAINQLRSLLVS
                     APQDVRDKLWKSKPHECVAACINCEVSEYSPLRIALYSTLRSLAKRWGALTEELNELD
                     ESLDKLTRKYASNLRSQFGVGPQTAATLLAVAGDNPERLKNEAALASLCGVNPLPASS
                     GKTVRHRLNRGGSREANNALWTIALVRMRSDPRTRIYVDRRTGEGLSTKEILRCLKRY
                     IVRELYPLILSDLTYANRPLLT"
     gene            13208..13393
                     /locus_tag="RHZ76_003000"
                     /pseudo
     CDS             13208..13393
                     /locus_tag="RHZ76_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611384.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         3371 a         3286 c         3561 g         3253 t
ORIGIN      
        1 gtggggaaga tgacgtaaat gtaatacagc cacgtacatt acacgattgt aatgaatttg
       61 tttcttaagg tgtgctagat tcatttcatt gtaagtggat gaaccagtaa tttaatttaa
      121 atcggttctc gaattctgtc agtaaccata ctttaaataa gggaatgcgc atgctgttga
      181 aaacgtctcg acgaactttc ctgaaggggt taaccctctc tggcgtagcc ggaagtcttg
      241 gcgtatggag tttcaatgcg cgttccagtc tgagcctgcc agttgccgca tccctgcagg
      301 gtactcagtt tgacctgacc attggtgaaa cggccgtcaa tatcacgggc agtgagcgtc
      361 aggccaaaac aatcaatgga ggcctgccgg ggcccgttct tcgctggaaa gaaggtgaca
      421 ccattaccct gaaggtcaaa aaccgtctta atgaacagac gtccattcac tggcacggca
      481 ttattcttcc ggccaatatg gatggtgttc cggggctgag ttttatgggc atagagcctg
      541 atgataccta cgtttacacc tttaaggtta agcagaacgg gacttactgg taccacagcc
      601 attccggtct gcaggaacag gagggggtat acggtgccat tatcatcgat gccagggagc
      661 cagaaccgtt tgcttacgat cgtgagcatg tggtcatgtt gtctgactgg accgatgaaa
      721 atcctcacag cctgctgaaa aaattaaaaa aacagtcgga ttactacaat ttcaataaac
      781 caaccgttgg ctcttttttc cgcgacgtga ataccagggg gctgtcagcc accattgccg
      841 atcggaaaat gtgggctgaa atgaaaatga atccgactga cctcgcggat gtcagtggct
      901 acacctacac ctatctcatg aacgggcagg ccccgctgaa aaactggacc ggactgttcc
      961 gtcccggtga aaagatacgc ttacggttta tcaacggctc ggcaatgacc tatttcgata
     1021 tccgtatccc cgggctgaaa atgacggtcg tggctgcaga tggccagtat gtaaacccgg
     1081 ttaccgttga cgaattcagg attgccgttg ccgaaaccta tgatgtcatt gtggagcctc
     1141 agggtgaggc ctataccatc ttcgcacaat ccatggacag gaccggttac gctcgaggga
     1201 cactggccac gagagagggg ttaagtgctg ccgttccccc cctcgatccc cgtcctctgt
     1261 tgaccatgga agatatgggt atggggggaa tgggacatga tatggcagga atggaccaca
     1321 gccagatggg aggcatggat aacagcggag agatgatgtc tatggacggt gctgaccttc
     1381 cggatagcgg gacatcctcc gcgcccatgg atcacagcag catggccggt atggatcatt
     1441 cccggatggc cggaatgccg ggtatgcaaa gtcatcctgc gtcagaaacg gataacccac
     1501 tggttgatat gcaggcgatg agcgtctctc cgaaattaaa tgatccgggt attggtcttc
     1561 gaaataacgg aagaaaggtt ctcacgtacg cggatttgaa aagccgcttt gaggatcctg
     1621 acggacgtga acctggccgt accatagaac tgcatttaac cggccacatg gaaaagtttg
     1681 cctggtcatt taacggaatc aagttttcag atgccgcacc ggtgctgctg aaatacggtg
     1741 agcggctcag gatcacgctg atcaacgata ccatgatgac tcaccccatt cacctgcatg
     1801 gtatgtggag cgatctggaa gatgaaaacg gtaatttcat ggttcgtaaa cacacaatag
     1861 atgttccccc tggtacaaaa cgcagttaca gagtgacagc agatgcgctt ggccgctggg
     1921 cgtatcactg ccatttgctc tatcacatgg aaatgggaat gtttcgtgaa gtccgggtgg
     1981 aggaatgatg cgaatgaaga gaaatttgaa ggccatacct gttctggtcg ccggtttgtt
     2041 tacctcacag ctttctattg cggcgggctc cgtctctgca gatccccacg ccgggcacga
     2101 catgtctgcc atgcagatgc cagcagatga gaatttcact gagatgacgt caatggagcc
     2161 cattgtaact gagagcagaa cgccaattcc gcctgttacc gatgccgacc ggaaggctgc
     2221 attcggcaat ttacaggggc atgcgattca cgacagtgcg attaattatc tggttctgct
     2281 ggatcaactg gaatggcaac ggtcggataa caccaacaat ttcagctgga gtgttaacag
     2341 ctggattgga ggcgacacag atcggatttg gctaaagagt gaaggtgaac gaagcaatgg
     2401 ggaaacggag gcggctgaag cgcagttact ctggggacat gcggttggcc catggtggga
     2461 tttggttgcg ggtgtcaggc aggatttcag acctgcttct gcccggacct gggctgctgt
     2521 cggttttcag gggctggcac tctataattt tgagtctgaa attacgggtt ttgtcagtaa
     2581 tggcggaaaa gcagcccttc gtctgggagg agaatacgac gttttactga ctaaccggct
     2641 catactccag ccatcctatg aggtgaattt ctacagtcag gatgatgaat cgcggggtcg
     2701 cggcagggga ctgactgaca cagagctggg gctccggctg cgctatgaaa tacgccgtga
     2761 gtttgcaccc tatataggcg tttcctggaa tcaactttac gggaaaacat ccgatatggc
     2821 gaaaagagaa ggtgagaaag accatcaggt agtattcctg gcgggagcca gaatctggtt
     2881 ttaacgcact gatataaaac actcaactaa acaggtaaat aaaatgtcga ttttaaataa
     2941 agccattctt acaggtggcc tcgttatggg cgttgctttc tctgctatgg cccatccgga
     3001 attaaaaagc tctgtgccac aggctgattc agccgtagcg gccccggaaa agattcagct
     3061 taatttctcg gaaaatctga ccgtgaaatt ctcaggtgca aaattaacga tgacgggtat
     3121 gaaaggcatg tcatcacatt ctccgatgcc ggtcgcggca aaagtggcgc caggcgctga
     3181 ccctaaatcg atggtcatta ttccgcgaga gcctttaccc gctggcactt atcgtgttga
     3241 ctggcgcgcg gtttcttcag atacgcaccc tattaccggt aattacacct ttacagtgaa
     3301 gtaatattat gaacgacctg attatgattg ttattcgttt tcttctttat ctggatttga
     3361 tggtaatatt tggattgcca ttttttcaga tatatggaat aagcggtgtc agacatgaaa
     3421 cctataacct gactaatttc aggtcgttta taacctttgc tgttgttaca ggcatcattc
     3481 ttactggcat taatatgctc ctggtatcta atgccatgag tggagtaact gacctcagag
     3541 aattatccat ccatgttatc gagatggtga tagaagaaac tgatgtgggt attagctgga
     3601 ttgtcaggct ctgtgccctg tttaccacac tcggtgcttt gttcctttac actaataaga
     3661 gagtattgtc ctgcctgctg atgacgatga gtgggggcgt ggcgctggct acacttgcct
     3721 ggggaggaca cgccgttatg catgacggtc tgcattacta tctccattta ctgagcgatc
     3781 tgacccatct cggcgctgca ggtgcctgga caggtgctct ggttgcattt gctatcctgc
     3841 tgatgcgcag aaacgagcat aatgcacaga gcgtcattgt gatatctgac tccctggcaa
     3901 aatttgccac tgcaggaacg gtgattgttg tagccctgat cctgagtgcg ctggtcaact
     3961 atctgtatat tgctgagggt aacttaactc ccttattcaa cagttcctgg gggaggatat
     4021 tgcttgccaa gacggctctg tttgttctga tgcttcttct ggctgcagca aaccggtttc
     4081 acctgggtcc ccggcttgaa gttatggtca gggaagggaa ttatgatcgc agcgttgccc
     4141 tgatgcgaaa cagcatcctg acagaattcg ttgttgcgat tatcattctg ggcgccgtag
     4201 cgtggcttgg aatgcttgct ccgtctcaga tcagctaggg gacagccaaa gcttatgcgt
     4261 gagattttta ctttcatatc agcgagttga ccatgcagcg tattttaatc gttgaagacg
     4321 aacaaaaaac aggtcgttac ctgcagcagg gactggttga ggaaggctat caggccgatc
     4381 tctttaataa tggccgcgat ggtctcgggg ccgcgtcgaa gggacagtat gatttgataa
     4441 tactggacgt gatgctgcct ttcctcgacg ggtggcaaat catcagcgca ctgagggagt
     4501 ccgggcacga agaaccggtc ctgtttttaa ccgcaaagga caacgtgcgg gacaaagtga
     4561 aaggactgga gcttggcgca gatgactacc tgattaagcc ctttgatttt acggagctgg
     4621 ttgcacgtgt aagaacccta ctgcgccggg cacgctcgca ggccgcaaca gtctgcacca
     4681 tcgccgatat gaccgttgat atggtgcgcc ggaccgtgat ccgttcgggg aagaagatcc
     4741 atctcaccgg taaagaatac gttctgcttg agttgctgct gcaacgcacc ggagaagtgt
     4801 tacccaggag tcttatctcg tccctggtct ggaacatgaa ttttgacagt gatacgaatg
     4861 tgattgatgt cgccgtgaga cgtctgagaa gtaaaattga tgatgacttt gagccaaaac
     4921 tgatccatac cgttcgcggt gccggatatg tcctggagat cagagaagag tgaggttcaa
     4981 aatttccctg accacacgcc tgagcctgat tttttctgcg gtgatgctta cggtatggtg
     5041 gttatcaagt tttatcctga ttagcaccct gaatggctat ttcgataatc aggaccgcga
     5101 ttttctgaca ggtaaacttc agctcaccga agagtttctt aaaacagaga cgttcaggaa
     5161 caaaacggat attaagtcat tatcagaaaa aataaacgat gcgatggtgg ggcacaatgg
     5221 cttattcatt tctataaaaa acatggaaaa tgaaaaaatt gttgaactct atgccaaaaa
     5281 ttctgttgtt ccagcggtcc tgcttaataa gtcgggtgat attctcgact atatgatcca
     5341 gacggaagaa aataacaccg tgtaccgcag tatctcgcgg cgggttgccg tgacgccgga
     5401 acagggtaaa agcaaacatg tcatcattac ggttgccacg gatactgggt atcacaccct
     5461 gtttatggac aaactcagta cctggctgtt ctggttcaat atcggtctgg tctttatttc
     5521 tgtttttctg ggctggctga ccacacgtat tggtctgaaa ccgctacggg aaatgaccag
     5581 tctggcttcc tccatgaccg tacacagcct ggatcagcgt ctaaatcccg atctggctcc
     5641 gccggaaatc tctgagacca tgcaggagtt caataatatg tttgatcgcc tggagggggc
     5701 attccggaaa ctgtcagatt tctcgtctga catcgcgcat gagctgcgca caccagtcag
     5761 taatctgatg atgcagacgc agtttgcact ggctaaggaa agggatgttt cgcattaccg
     5821 cgaaatttta ttcgctaacc tggaagaact gaaaaggttg tcacgaatga ccagtgacat
     5881 gctttttctg gcacgttcag agcatggtct gctgcggctg gataaacatg atgtggatct
     5941 ggcagccgaa ctgaatgaat tacgtgagtt gttcgagccc ctggcagacg aaacaggaaa
     6001 gacaatcacg gttgaaggag agggcgttgt tgccggagac agcgatatgc tccgacgtgc
     6061 tttcagtaac ctgctttcca atgcaatcaa gtattctccc gataacacct gtacagcgat
     6121 acaccttgag cgtgacagtg actgtgtgaa cgtgatgatt acgaatacga tgtccggcca
     6181 ggttcccgct aatctggaac gtttgtttga ccggttctat cgcgcagact catcaaggtt
     6241 ctacaacacg gaaggcgcgg ggctgggatt atcaattaca aggtcgatca ttcatgctca
     6301 cggcggcgag ctgtcagcag aacagcaggg gcgggaaatt gtgttcagtg tgcgtctgtt
     6361 aatggattaa taccgttatt caggagaaac ccggaaggtg acaaaaatgt catcgttcag
     6421 tcacgcgata aacagaggcg gttttttata atcagccata aatcagggca gcgtgataat
     6481 tcaatcgccc ggttcctggc gtgatgatca accagccctg agatcaaatg ctttctctgt
     6541 tataagcatt gattgttcgg gtatgaaaac accggagacc caaccatgaa aaagatcctt
     6601 gtatccttta ttgccatgat ggctgtcgct tcatctgcct gggctgcaga gacaatgaac
     6661 atgcatgacc aggtaaataa tgcccaggcg cctgcccatc agatgcaatc atccgctgaa
     6721 aaaagtgcag ttcaggggga gagcatgaaa atgatggata tgagcggtca cgatcaggcc
     6781 gcaatgtccc atgaaatgat gcaaaacggc aattctgctg cccatcagga catggctgaa
     6841 atgcataaaa aaatgatgaa atccaaacct gagaacaccc aagaaactgc taagccattt
     6901 tccgaaatga acgagcatga gaaagccgct gttgtgcatg agaaggcgaa taatggtcta
     6961 tcttccgtta ttcatcagca gcaggctgaa aagcatcgca gccagatcac ccagaattaa
     7021 cccgcagctc cacttgtcag accctcattt gacgccgaag tcactggctt acgctcccgt
     7081 ccgggagcgt ttttttccca tatatcaaac tttaactctg aagaggtgga agtatctgac
     7141 caacactgtc acgtaacgcc agataactac aaaaacacct caggtgaacg taagcgttaa
     7201 gtgagcgccg tattgacggc tatttattgg tgagatctac gttccatggc agcagttcgg
     7261 ccactttgtt gatgggccac tccggcaata cgctcaggat atggcgaagg taaccctctg
     7321 gatcgatacc gttcagcctg cacgttccga tcagaccata cagcagggca ccacgttcac
     7381 caccatgatc actgccgaag aagatgtagt ttttcttgcc cagacagacc gctcgtagcg
     7441 cgcgctcagc agcgttatta tccggctctg ccagaccatc atcgcagtag taacacaggg
     7501 catcccactg gttcagtgca taagcgaacg cctcgcctaa ccgggatttt ttcgacagag
     7561 tggcactttt ctccaccaac cagtcatgca gggatatcag caacggttaa cttcgggacc
     7621 gtctggcggc cagccgctga gatgccggaa ggccgcgtat ttcctcttct atcgcgtata
     7681 actcactgat gcgcttcaga gcctcctctg ctgtggccgt ccgggtgctg atatagacgt
     7741 catggatttt gcgccgcgca tgagcccagc atgccgcttc tgtcaacggg ccaccttcac
     7801 gctctgcgct gaacaacctg tcgtaccctg cgaaggcatc tgcctgcagc acgccatgat
     7861 agtgccgaag atgttgctga gggtgtttcc cctgtcggtc cggtgagaag gcgaaccatg
     7921 ccgctggcgg atctgatgaa cccgcgcttc tgttatcacg gacatatgtc cagatacgcc
     7981 cggttttcgt cttctttctg cccggcgcca gcacgggcac cggagtgtca tccgtatgca
     8041 gtttgcggca gtccatcacg tagtggtaga gggcttcatc cagcggggcc attaaccggc
     8101 agcacgcatc cacccagttg gagagcagcg cacgactcag atccacaccc tgacgggcaa
     8161 agatttcgca ctggcgatac agcggcaggt gttcacagta tttggccgtt aacacgcggg
     8221 ccagcagacc cggcccggcg atgccccggt cgataggacg tgagggcgct ggcgcttcaa
     8281 cgacgcagtc gcatcgggta caggcctttt tcaccctgac cgtgcggatc actttcaggg
     8341 cgctggagac cagctccagt tgctccgcgc tgacttcgct gagataggcc atatcactac
     8401 cgcaaccagg acagctggtt tcagctggct ccagtctgtt tatttcgcgg ggaagatgtt
     8461 ccggtaacgg gcgacgatga cgggactggc gcaactgacg cggaacctgc tgatcattgt
     8521 cccggccgtt gtaacgatcg ctttgctgct cctgctgttt cagcagggct tcggcttctt
     8581 cgacctgccg gcgtagcttt tctgaacggg taccgaacag cattcggcgc agtttttcta
     8641 tctgagcccg cagatgttct atttcacgct cttcgttctc tattttctct tctgcgcggg
     8701 ccagcgctga acgcaggaac gactcagtct cagcaacgag actgagctgg ctgtctttct
     8761 ggcgaagcgc gtcttccagc gcagcgatac gggcgagata gtcctgactc atgaccccat
     8821 ttataatgcg gtcatgcgct ttttacaaca tcgtcagtga gttaaggcgt gcagttttag
     8881 gtttccgcca gtccagctta tcgaggagca tggcgagttg tgagcgggtg atggcgattt
     8941 tgccgtcgcg tacagcaggc cagacgaact gtccctcttc cagacgtttg gtaaacagac
     9001 acagaccgtc agcatcagcc cagaggatcc tcacggtgtc acctttacga ccacggaaga
     9061 taaacaggtg gccggagaac ggattgtcat caagaacatg ctggaccagt tcgcccagac
     9121 cattgaagga cttacgcata tcggtgaccc cggcaaccag ccagatacga gtgcctgacg
     9181 gtaagggtat catcggctcc tcccgctcag ctcgcggatc agcgttttca gaagtgcagg
     9241 tgataaatcc ccggacagac gcaggctggc accgccgaca gtcacctcgc atgtcatggc
     9301 ctcgcggcag gctggagcgg cgggttcata gtgcggctca gcatggtgtg acaggaccat
     9361 atcaggcagg acaacgggga taacagagtc ttgctctctg attgaccgtt gtatttcatc
     9421 gggcagaaga tgtctgtaac gctggcgcca ggtaaacagc agattgtcat taattccatt
     9481 atcccgcgcc aaccgggcaa cacaggctcc aggctgatat gaagtggttt actgaatttg
     9541 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgtcat aatgaaaaaa
     9601 agaaatttca gcgcagagtt taaacgcgaa tccgctcaac tggtcgttga ccagaactac
     9661 accgtggcag atgcagccag cgctatggat gtcggccttt ccacaatgac gcgatgggtg
     9721 aaacaattac gtgatgaacg gcagggcaaa acaccaaaag cctcccccat taccccggaa
     9781 caaattgaaa tccgtgagct caggaaaaag ctacaacgta ttgaaatgga aaatgaaata
     9841 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
     9901 aactcagggc gcgttatcct gtggccactc tctgccatgt gttcggggtt catcgcagca
     9961 gctacaaata ctggaaaaac cgtcctgaaa agccagacgg cagacgggct gtattacgca
    10021 gtcaggtact tgaactgcat ggcatcagcc acggctctgc cggagcaaga agcatcgcca
    10081 caatggcaac ccagagaggt taccagatgg ggcgctggct tgctggcaga ctcatgaaag
    10141 agctggggct ggtcagttgc cagcagccga ctcaccggta taagcgtggc ggtcatgagc
    10201 acgttgctat cccgaatcat cttgagcgac agttcgccgt aacggaacca aatcaggtgt
    10261 ggtgcggtga tgtgacctat atctggacgg gtaagcgctg ggcgtacctc gccgttgttc
    10321 tcgacctgtt cgcaagaaaa ccagtgggct gggccatgtc gttctcgccg gacagcaggc
    10381 tcaccatgaa agcactggaa atggcatggg aaacccgtgg taagcccgtc ggggtgatgt
    10441 tccacagcga tagtaataat gccgatgtca gtctttacca ccacttagtt tgccgtttaa
    10501 ccagattggt gtgattactg atgcagtgaa gaccttcccg catcctgact cacacagcga
    10561 tcgacctttt gtgtcctgcc ctggacccgt cggttgccgg aagcgccttc atgcgaggca
    10621 tctcctcacc gatgcgcgtg actcaagaag ggcctgacgg cttgtctcgt tactgtcctg
    10681 tccgggttat ctgtctggag attcaactct gtttcctcac aggagctctg tcatggttga
    10741 taaagttacc gaagctgccg ttgtgggtgg cgtggataca cataaagatt tgcacgttgc
    10801 cgctgtcgta gatcagaaca ataaagtcct tgggactcag tatttctcca caacacgaca
    10861 aggttaccgg cagatgctgg catggatgac ctcgtttggg acattaaagc gaattggtgt
    10921 tgagtgcaca ggtacctatg gttccggttt gcttcgttat tttcagaacg ccgggttaga
    10981 agttcttgag gtgactgctc cagaccggat ggagcgacgc aaacggggta aaagtgacac
    11041 aattgatgct gaatgtgccg ctcatgcagc attctcaggc atcaggacag ttactcccaa
    11101 aacgcgtgat ggcatgattg agtccctgcg ggtattaaaa acttgccgaa aaacagcaat
    11161 atcagcccgc agagtcgctc tccagattat ccattcaaat attatctctg ccccggatga
    11221 attacgtgaa cagctcagaa atatgacgcg catgcagctc atcaggaccc tgggttcctg
    11281 gagacctgat gccagtgaat accgcaatgt aaccaacgtt tatcgcattt cattaaagtc
    11341 ccttgcccga cgctatctcg agttacatga cgaaatcgct gatctggatg tcatgattgc
    11401 ggcaattgtc gacgagttgg cacctgagct gattaaacgt aatgctatcg gatacgaaag
    11461 cgcttcacag ttgctgatca ctgccggaga caatcctcaa cggttaagat cagaatcagg
    11521 atttgcggca ctgtgtggtg tcagcccggt ccccgtttct tctggaaaaa cgaaccgtta
    11581 ccgacttaat cgtggtggag atcgtgctgc aaatagtgca cttcacatca tcgccatcgg
    11641 acgcttacga actgacgcaa aaacaaagga atatgtcgcc aggcgtgtag ccgaaggaca
    11701 tacaaaaatg gaagcgatac gctgtctaaa gcgatatatc tcccgcgaag tttacacgtt
    11761 actgcgcaat caaaacaggc ggatcaacag tatcccgata acagcttgac tcttagaagg
    11821 gcgtccaggg cagtcagtat ggcagtgatg actggcagcg gttctgccgg gccaataatc
    11881 tggtaccgag catgagccgt cgcggcaact gctgggatac tcattctaat atactttcaa
    11941 ataccggatc ggactgaccc gtgctgggat tggctcgaat gcctttgtgt tgacctgtcg
    12001 tccgattctg gtatttcccg ggcatagccg ggcccagcgt ttgtgacctg ataggagctt
    12061 tgtgctgcat cttgagtgtc ctgtcctgcg atggggatgg ttgtgctact gatatctggg
    12121 gggtaaacca tgcaaaacaa ttttcaatct cctgaaagcg tgatcctggg tgtcgataca
    12181 caccttgata ttcatgtcgg tgctgttatc agtcaggcgg gtaagctgct tggtactcgc
    12241 attattcaaa caaatcaaat tggctatcag gagctatttg attgggctaa atcttttgga
    12301 gttctcgaac gagcaggtat tgaaggaacc ggaacttatg gtgctgcatt aacacgtttt
    12361 cttattaaaa atagtattta tgttgttgaa gttaaccgac cagaccgctc aaaacgccgc
    12421 cttgaaggaa agtcagatcc actggatgca gagaatgcag cccgcaccgt actttcgggg
    12481 agcagcaaag ccatacccaa aatgcaatcc ggcgcgtgtg aagctctccg gataattagc
    12541 gttgcccgac gtagcgctgt aaaagcccgt actcaggcga ttaaccagct tcgttcgctg
    12601 ctggttagtg caccgcagga tgtccgcgac aaactctgga aatcgaaacc acacgagtgc
    12661 gtagctgcct gtattaactg cgaagtatca gagtactctc cactgcgtat agctctgtac
    12721 agcacgctta gaagccttgc gaaacgatgg ggggcactga ctgaagagtt gaatgaactg
    12781 gacgaatcgc tggataaact gacccgtaaa tatgccagta accttcgtag ccagtttggc
    12841 gtagggccac agacggcagc cacactgctg gcagttgcag gtgataatcc cgaacgttta
    12901 aaaaatgaag cagcactggc atcgctttgt ggcgtaaatc cgctgccagc atcatcagga
    12961 aaaacagtca ggcacagatt aaatcgaggt ggctcacgtg aagcaaacaa tgccctgtgg
    13021 acaatagccc ttgtgcgtat gcgaagtgat ccgcggacaa gaatctatgt cgacagacga
    13081 actggtgaag ggctatcgac caaagagatc ctgagatgtc taaaacgcta catcgtccgt
    13141 gaactctacc cattgatttt atcagatctc acatatgcca atcgcccgct cttgacatag
    13201 gagcgtcaat gcggtggccg aatcgttctt cagttcactg aaaaaagagc gcatcaggaa
    13261 gagaatatac aaaacccggg atctagcccg ggcggatatc ttcgattaca ttgaagtgtt
    13321 ctacaaccgg gcccggcgcc acagtcacct cggcggcgtc agtccggagg ccttcgaaca
    13381 ggcctcgtcg tgaggacaga atttgtctac gggcgtgggg tcagtccaat caacggctcg
    13441 gaagtgtcta gattatccgt ggcgattcaa t
//
LOCUS       DAPHBJ010000116        13413 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.117, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000116 DAPHBJ010000000
VERSION     DAPHBJ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.117"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(262..687)
                     /gene="arsC"
                     /locus_tag="RHZ76_003001"
     CDS             complement(262..687)
                     /gene="arsC"
                     /locus_tag="RHZ76_003001"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HDV2813600.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(700..2010)
                     /gene="arsB"
                     /locus_tag="RHZ76_003002"
     CDS             complement(700..2010)
                     /gene="arsB"
                     /locus_tag="RHZ76_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HDV2813601.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALFVTLAALALRLSFTL"
     gene            complement(2064..2417)
                     /gene="arsR"
                     /locus_tag="RHZ76_003003"
     CDS             complement(2064..2417)
                     /gene="arsR"
                     /locus_tag="RHZ76_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HDV2813602.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            3157..3240
                     /locus_tag="RHZ76_003004"
     CDS             3157..3240
                     /locus_tag="RHZ76_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HDV2813603.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3294..4646)
                     /gene="gorA"
                     /locus_tag="RHZ76_003005"
     CDS             complement(3294..4646)
                     /gene="gorA"
                     /locus_tag="RHZ76_003005"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDV2813604.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4718..5560)
                     /gene="rlmJ"
                     /locus_tag="RHZ76_003006"
     CDS             complement(4718..5560)
                     /gene="rlmJ"
                     /locus_tag="RHZ76_003006"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HDV2813605.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARLLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGIYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5763..7805
                     /gene="prlC"
                     /locus_tag="RHZ76_003007"
     CDS             5763..7805
                     /gene="prlC"
                     /locus_tag="RHZ76_003007"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HDV2813606.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7813..8565
                     /gene="rsmJ"
                     /locus_tag="RHZ76_003008"
     CDS             7813..8565
                     /gene="rsmJ"
                     /locus_tag="RHZ76_003008"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HDV2813607.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8614..10083)
                     /gene="dtpB"
                     /locus_tag="RHZ76_003009"
     CDS             complement(8614..10083)
                     /gene="dtpB"
                     /locus_tag="RHZ76_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HDV2813608.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(10401..10835)
                     /gene="uspA"
                     /locus_tag="RHZ76_003010"
     CDS             complement(10401..10835)
                     /gene="uspA"
                     /locus_tag="RHZ76_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HDV2813609.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11226..11561
                     /gene="uspB"
                     /locus_tag="RHZ76_003011"
     CDS             11226..11561
                     /gene="uspB"
                     /locus_tag="RHZ76_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HDV2813610.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11806..13305)
                     /gene="pitA"
                     /locus_tag="RHZ76_003012"
     CDS             complement(11806..13305)
                     /gene="pitA"
                     /locus_tag="RHZ76_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HDV2813611.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
BASE COUNT         3391 a         3475 c         3505 g         3042 t
ORIGIN      
        1 cgtctttaaa ctactgcgct gtatcgctga taactaagca gctcactgat acaccatgct
       61 ggacgcgata attggaatct cacgcaggga gtactgtgta aatagcaaaa tcgcctgatg
      121 cgctacgcct gtcaggccta caaataacat actattcatc gaattttcat aactttgtag
      181 gtcggataag acgcgccagc gtcgcatccg acataaataa agcgcacttt tctaacaacc
      241 tgtggggggg atatcgccgc tttatttcag gcgcttaccc gcttcatcaa ccactttctc
      301 gccatcttcc ttggagaatg cgcctttttg cgcatctggc agaatttcca gcaccacttc
      361 tgaagggcgg cacaggcgag ttcccagcgg cgtcaccaca atcgggcgat taatcagaat
      421 cgggtgctga agcataaagt cgattaaccg atcgtcagta aatttatctt ccgcaaggcc
      481 cagctcctca tacggttcga cgtttttacg cagcagcgcg cgtacggaaa tccccatatc
      541 ggcaatgagt ttgaccagtt catcgcgcgt tggcggagtt tccagataat ggataatggt
      601 cggttctgtg ccgctgttgc ggatcatctc cagcgtatta cgcgacgtgc cgcaggccgg
      661 gttgtgataa atggtaatgt tgctcatatc agtatctcat tacaaagtga aagagagacg
      721 tagcgccagc gcagccagcg tcacaaacag cgcagccagc gtcacaaaca gcacaggcag
      781 ggtcatgata atccctgtac ggaagtaata tccccagctg atagtcatat tcttctgcga
      841 aagtacgtgc agccagagta gcgtagccag gctaccaatt ggggtaattt tcggtcccaa
      901 atcgcagcca atcacattgg cataaaccat cgcttctttg ataacgccag atgccgtgct
      961 gccatcaatg gacaacgcgc caaccagtac cgtcggcata ttgttcataa tagaagagag
     1021 gaaggcggtg aggaatccgg tgccgagcgt cgcggcccac aggccgttat ccgccagcac
     1081 gttgagtacg ccagaaagat attccgttaa tccggcattg cgcaggccat aaaccaccag
     1141 atacatgccg agcgagaaga tgacaatctg ccagggggca ccgcgcagga ctttacccgt
     1201 attaatcgca tgaccgcgtt tagcgacgac aaataatatc agcgcgccca cagctgcaat
     1261 ggcgctcacc ggaatgccga gcggttccag gacgaaaaat cccaccagca gaagcagtaa
     1321 aacaacccag ccagttttga acgtagcagg atctttgatc gcttctgcgg gagatttcag
     1381 cagcgccata tcgtagttct gcggaatatc tttgcgaaaa tagagatgta gcatcaccag
     1441 cgtggcaaca atcgcggcga tatccaccgg caccatcacc gaggcgtatt cgcgaaagcc
     1501 gaggccaaag aaatcagcgg aaacgatatt caccaggttg gagacaataa gtggcaggct
     1561 ggcggtatcg gcaatgaatc cggccgccat cacgaacgcc agcgtagtgc ctttactgaa
     1621 ccctaaagcc agcagcatgg cgatgacaat cggtgtcaaa ataagcgccg cgccatcatt
     1681 ggcaaacagg gcggcaacgg cagcaccgag caggacaatc caggtaaaca gcaagcgacc
     1741 acgaccatta ccccagcgtg agacgtgcag cgccgcccat tcaaaaaagc cggactcatc
     1801 cagcagcagg ctgatgataa tgacggcgat aaacgcagcc gtcgcgttcc agacgatatt
     1861 ccacaccacc ggaatatcac ccggatggac cacgcccgta actaacgcca gtactgcgcc
     1921 gagcgttgca ctccagccga tgcctaaacc tttcggctgc cagataacca atacgatggt
     1981 caggacaaag atagcgcctg ccagtaacat aatgcctccc ggataaaaca catctgaaaa
     2041 ttcatatgtg tttagctaaa tttttaactg caaatgttct tactgtcccc ggaacagttt
     2101 tgtcgagcca ggttgcggac aattgcctga atcttttcct gttcacatcg ccaggcctca
     2161 tcaataattt tcgccgccca tgctggaata tgcggtgata agcggtaatg aacccactta
     2221 ccttgcttgc ggtccagcaa tagcccgctt tcacgcagca atgccaggtg gcgggagatc
     2281 ttgggctgcg actggtcgag agcagtgcag agatcgcaga cgcataactc tcccagttcg
     2341 ctgagcagta aaacgatgcc cagacgggtt tcatcagcaa gaattttgaa caattggatg
     2401 ggtaacagaa atgacatatt gcgctcctga ttgttgcagg tagtgtctct cttcgaagcg
     2461 gataagtcaa aaacatatat gacttaacga atgtgtaagt gcagaggaag gtaataggtg
     2521 tgaattttga gttggctatt catttgaaag gaggtctgaa tcaggacgta aaaaaaccga
     2581 ctttgcgtcg gttttttact ttccagccct gagttggtgg ctctggctgg agtgagagag
     2641 ttcttatcta acagctcaat actattagcc agtggctaac attgcaagaa ttagcgattt
     2701 cttcagctgg cggagggagc acttcggtat atatcgcagt gacgatttat atactttcac
     2761 tgggtcatcg tcatattaag ccttgtccga atccggatgt aaaaaaaccg actttgcgtc
     2821 ggttttttac tttccagccc tgagttggtg gctctgactg gagtgagata gccatcatct
     2881 aacgcatcaa tgctaatacc atactgaaac tattgcaagg acgtgctggt tttataacct
     2941 gcatgtactg tatgattatc cagttagctc tgaggcattt tcactctggc aatgcgcata
     3001 aacgctttca aagtcctggt cagaagtacg ggtggtgccg ttaactgatg ctctggccgg
     3061 agtgagagag ttcttatcta acaatgagac atgcgccgtg acaggcagtg gatgagtaag
     3121 cggatgcatt ctcactccat cgcatggaga aaacgggtga ttgataaagc aatcatcgtt
     3181 ctaggggcgt taattgcgct gctggaactg atccgctttc tgcttcagct tctgaactga
     3241 tagcggaaac gtaattaagg gctaagagca cactactctt agccctttaa catttaacgc
     3301 attgtcacga actcttctgc cgccgttggg tgaatggcga cggtattgtc gaagtctttt
     3361 ttggttgccc ccatcttcag cgccaccgcg aagccctgca acatttcgtc cataccaaag
     3421 ccaatgccgt gaataccgac aatcttctct tccgatccaa cgcacaccag cttcatgcgg
     3481 cacggctggc ggtgagtggt gacggcggta tacatcgcgg tgaaagagga tttatacact
     3541 ttcacctgat cgtcgccata ctgctcgcgc gcctgcggtt ccgttaaacc aacagtacca
     3601 atcggcggat ggctgaagac cacggtcgga atgttgctgt aatccagatg ctcatccggc
     3661 ttgttattaa acaggcgttc agagagacgg cgacccgctg caactgctac cggtgtcagt
     3721 tctactgcac ccgtgttatc gccgaccgcg taaatacctt caacgttggt gttttgatat
     3781 ttatcgacga cgatatagcc tttttcgttg gtttttacac cagcggcttc caggttgatg
     3841 ttgtcattgg caggctcgcg accaatcgcc caaatcaggc aatccaccgt ttcactgcga
     3901 ccatcttcca gctccagcgt caggctacca tcggcatttt tcactaccgc tttcgggatg
     3961 gcgttggtat gcaactgtgg gccttcggcg ttcatcactt cgaccagcgt ttcagagatc
     4021 atcgggtcga agctgcgcag cggcgcatgt ttacgcacaa acagatgcgt tttcgcgccg
     4081 aggccgttaa tcacgccagc cagctcaacg gcgatgtaac ccgcgccaac aaccgccacg
     4141 cgctctggca aagcaggaag ggcgaagaag ccatcggaat caataccgta ttccacgccc
     4201 ggaatatccg ggtggctcgg acgaccgccg gtggcgatca gaatatgatc ggccgtgatg
     4261 gtttcaccgt taacttccag cgttttggca tcaacgaagc gggcaaagcc tttgattaca
     4321 tcaacgttat ttttaccgag cacgttttca taggaagtat gaatacggtc gatataggcg
     4381 gtacggctgg cgatcaacgt ttcccagttg aatttattga tagtggtatc aaaaccataa
     4441 tccgggccgt acatatggat cgcttcacgg atttgcgccg cgtgccacat cacttttttc
     4501 ggcacacagc caacatttac gcaggtgccg cccagctctt tggcttcaat cagcgcacat
     4561 ttctggccgt acatagccgc gcggttgatg gaggcgatac cgccgctgcc gccgccgatg
     4621 gcgatgtaat catagtgttt agtcatgaca aagtgtcctt atcgttgatt accgcgattg
     4681 tagcgcgaga cgtaataggt gccagcaatg gctgcaatta ctccggcacg atccagctta
     4741 cggtggcgtg cccggtgcct gccggaacca gtttgctgtg cagccacggc agcacgttat
     4801 tcatctgttg ttccagtttc cacggcgggt taatcacaat catgccggaa gcggtcatgc
     4861 cacggcgatc gctgtctggc agtaccgcca gttcaatttg cagaattttg cgaataccgg
     4921 tcgcttccag atcgtggatc atgcgcttaa tttgctgacg cagcaccacc ggataccaca
     4981 gtgcgtaaat accagtggcg aaacgtttgt aaccttctgc tatcccgctg accaccgctt
     5041 gatagtcagt tttcatttca tacggcgggt cgataaggat taaaccacgg cgggaaaccg
     5101 gcggcagttt ggccttaagc tgctggaaac cgtcggcttt ttcgacacgc gcacggctat
     5161 ctttctgaaa ttcagaacgc aacaacgggt aatcgctcgg gtgcagttcg gtcagttgca
     5221 ggctgtcctg ttcacgcagt agcaggcgag caatcaacgg cgaaccgggg tagtaacgca
     5281 actgaccgct acggttgaag tgttttacca cattgatgta cgcctccagt tctgcgggca
     5341 aatcgtcctg ctgccagata cgggcgatgc cttcgagata ttcgccggta cgctcggcat
     5401 gttcgctgcc taactgataa cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt
     5461 tatctttctc tttcagcgac tcgatgatca ggctctgaac ggtatgttta aggacgtcgg
     5521 cgtggttgcc agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt gttccggtaa
     5581 gtaaaaatcg gcccgtattc ggggcgcaca aaagcgtatc aggacagtat accgaaaaga
     5641 ggccgccgcc gcgaaagcgt aacgtttctc attgaaattc actacactta accccatgct
     5701 acacacatta tgtaaagcgc ctgttgagcg cttccttaac ctctttaacc aggactgcgc
     5761 gaatgacgaa tccgttactg actccctttg aattgcctcc gttttctaaa attctcccgg
     5821 aacatgtcgt tccagccgtg actaaggcat tgaacgactg ccgcgaaaac gtggagcgcg
     5881 tagtagcgca aggggcaccg tacacctggg aaaatctctg ccagccgctg gcggaagtgg
     5941 acgatgtgtt ggggcgtatc ttctccccgg tcagccacct gaactcggtg aaaaatagcc
     6001 cggaactgcg tgaagcctac gaacaaaccc tgccgctgct gtcggaatac agcacctggg
     6061 tagggcaaca tgaagggctg tataaagcgt atcgcgacct gcgcgatggc gatcattacg
     6121 ccacgctgaa cacggcgcag aagaaagcgg ttgataacgc actgcgcgac tttgaactct
     6181 ctggcatagg tctgccgaaa gagaaacagc agcgttacgg cgaaattgcg acccgtcttt
     6241 ctgaactggg caaccagtac agcaacaacg tcctcgatgc gacaatgggc tggaccaaac
     6301 tcgttaccga cgaagcggag ctggcgggga tgccagaaag cgcgctggct gcggcaaaag
     6361 cccaggccga agcgaaagag ctggaaggtt atttgctgac gctggatatc ccaagctact
     6421 tgccggtaat gacctactgc gacaaccagg ctctgcgtga agagatgtat cgcgcttaca
     6481 gcacccgcgc ctccgatcaa ggcccaaacg ccggtaaatg ggataacagc aaggtgatgg
     6541 aagagatcct cgcgctgcgt cacgaactgg cgcaactgct gggctttgaa aactacgcct
     6601 ttaaatccct tgccactaaa atggcagaaa acccgcagca ggtgctggat ttcttaaccg
     6661 atctggcaaa acgcgcgcgt ccacaaggcg aaaaagagct ggcgcaattg cgtgcctttg
     6721 ccaaagccga atttggcgtc gatgagttgc agccgtggga tatcgcttac tacagcgaaa
     6781 aacaaaaaca gcacctctac agcatcagtg acgaacagct gcgtccgtac ttcccggaaa
     6841 acaaagcggt taacggcctg tttgaagtgg ttaagcgtat ttacggcatc accgctaaag
     6901 agcgtaaaga tgttgatgtc tggcatccgg atgtacgttt cttcgaactg tatgacgaaa
     6961 ataacgaact gcgcggtagc ttctacctcg atctgtatgc ccgtgaaaac aagcgcggcg
     7021 gggcgtggat ggatgactgc gtaggccaga tgcgtaaagc tgatggttct ttgcaaaaac
     7081 cggtcgcgta tttgacttgt aacttcaacc gcccggtaaa tggtaaaccg gcgctgttca
     7141 ctcacgacga agtgatcacc ctcttccacg agttcggtca cggcctgcac catatgctga
     7201 cccgcatcga aaccgctggt gtttccggta tcagcggtgt gccgtgggat gcggtcgaac
     7261 tgccgagtca gtttatggaa aactggtgct gggagccgga ggcgctggcg tttatctctg
     7321 gtcactatga aaccggcgaa ccgctgccga aagagttgct ggataaaatg ctggcggcga
     7381 agaactacca ggcggcgctg tttattctgc gtcagctgga gttcggcctg tttgatttcc
     7441 gccttcatgc cgagttccgc ccggatcagg gggcaaaaat cctcgaaact ctggcagaaa
     7501 tcaagaaact ggttgccgtg gtgccatctc cgtcctgggg ccgtttcccg cacgctttca
     7561 gccatatttt cgccggtggt tatgccgcag gttactacag ctacctgtgg gctgacgtac
     7621 tggcggcaga tgctttctcg cgctttgagg aagagggcat tttcaaccgt gaaaccgggc
     7681 agtcgttcct cgacaacatt ctgagccgtg gcggttcaga agagccgatg gatctgttca
     7741 aacgcttccg tggtcgtgaa ccgcagctgg atgcgatgct ggagcattac ggcattaagg
     7801 gctgatcatt cagtgaaaat ctgcttaatt gatgaaacag gcaccggaga cggtgcctta
     7861 tctgttctgg cggcccgctg ggggctggag cacgatgaag acaacctgat ggcgctggtg
     7921 ttaacgccgg aacatctgga attgcgcaag cgtgatgagc caaaacttgg cggcatcttt
     7981 gttgattttg ttggcggagc gatggcgcac cgacgcaaat tcggcggtgg tcgcggtgag
     8041 gcggtggcga aagcggtggg cattaaaggc gattatttgc cggatgtggt ggatgccact
     8101 gcaggacttg ggcgcgatgc ctttgtactg gcttcagtcg gctgccgcgt gcggatgctg
     8161 gagcgtaatc cagtggttgc cgcgctactc gacgacggcc tggcgcgtgg ttatgcggat
     8221 gcggaaatcg gcggctggtt gcaggagcgg ttgcagttaa ttcacgcctc cagcctgacg
     8281 gcgctgactg atattacccc gcgcccgcag gtggtttatc tcgacccgat gttcccgcat
     8341 aagcagaaaa gcgcgctagt gaaaaaagag atgcgtgtct ttcagtcgct ggtgggaccg
     8401 gatcttgatg ccgatggatt actggagcct gcacgcttgc tggcgaccaa acgcgtggtg
     8461 gtaaagcgcc cggactacgc gccaccgctg gcgaatgtcg ccacgccaaa cgcggtagtc
     8521 actaaagggc atcgctttga tatctatgca ggtacgccag tgtaaaacta aaacagggta
     8581 gcgataacgc taccctgctt tctgcaagaa taattaatgg ctttccggcg tcgcaatcat
     8641 gcgtttcagc cacggcacca tcaacagcat cactactgca acgcccagcg tgaccagacc
     8701 aatcttacca aacacgttgg tatagacggg caacgtctca agcggatcgg taatgttgtc
     8761 cggcaccgcg gtaaatgttg ccacatagcc gcccagcaag aacgcggcag cctgcgtcag
     8821 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
     8881 caggccaagg gcgctaataa acagttcacc taagctctgg aataagtaca ccagcacgat
     8941 aaaccatggc gatgtcagcc cttgtgcatc cgcaaaccac attcccgcag ctgccgccgt
     9001 caaaaagccc agtgagcaca taaacatgcc gagagtaaat ttcatcggca tcgagaggtc
     9061 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
     9121 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
     9181 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
     9241 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
     9301 atccagcttg aatgcctgac gaaagaagat gatggtgacg acgatggaga gaacaatcag
     9361 caccagattg gcgacttcta cgttgtgcat cagccatgcg catacgaaga tcatcaccac
     9421 gctgccaagt aacacgtaca acagtttgct gaagctcatt ggccggaagt cgggttcaga
     9481 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
     9541 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
     9601 cgataacgct atcaacgagc cgatgttgat cgacatatag aacagggtga atgcgccatc
     9661 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
     9721 caggccgtta cctacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
     9781 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gcaccaagaa caatggtgcg
     9841 tttggtcccc agcaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
     9901 cgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
     9961 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
    10021 aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt
    10081 catatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
    10141 gaaaaagttt acatctgtca gagattctta atattgatgc ggtgaataga ttaatacact
    10201 attacaacag aaaataacca gctgatcaca ctaatgatgg aaaatatgat ggtgttaaat
    10261 attgtattgg ctggggattt tgattgcgaa cgcagaccgt aggccagata aggtgtttac
    10321 gctggatcag gtaacaaggc gcgccaaaaa gcaaaacgcc cgcatatgcg ggcgttcagg
    10381 aacacgtcgt agagggaaga ttattcttct tcgtcgcgca gcggaacaat cagcatatca
    10441 acgtgaacgg tgttgatcag ctgacgtgcg gaagacatca gtttgctcca gaagtcctgg
    10501 tggtgaccac aaaccaccag atccatatcg tatttcttga ttgcatcgac cagaacttgg
    10561 cccaggtcgc cgctgccgct cagggtttca gtgattgggt agcctgcatt agtggaaagc
    10621 tcggtcagcg catgatgtgt ctcttctgag atgcgtttct gcatatcacc cagattcaca
    10681 tcaataagcc cggtgtatag gtcagagtag tttacatcta cgtggatcag agaaactttc
    10741 gcattgtagg ggcgagccat agagactgct ttctctacca gaactttgct ttccggggag
    10801 agatcgaccg cgatgagaat gtgtttataa gccatagtgt tactccttcc ataaagttgt
    10861 cgatgactgg ccagctagcg tttcttgtgc cgttacgtca cccgcgtcct gcgaactacg
    10921 tgcgcggggc taacccagcc gtaaagattc agtgttaaga ccatccttac cttatagcga
    10981 cccggatgat ccgtcaatcc gccttgctta cccgttagat aaacaaattt tcgacatgaa
    11041 tatattgata gtggttaacc ttctggaaaa aaaacaacct gatctcctac actatctata
    11101 gagccgctcg gatgttggtc accacaaaag cggtttctcc aggacctggt gttaactgat
    11161 tggcagcgta tcgggagcgg tagccccggg gtggaactcc gcgggcaggt cgccggggag
    11221 gaggtatgat aagcaccgtc gcattatttt gggctttatg tgtcgtttgc attgttaaca
    11281 tggcgcgcta tttctcatca ctacgcgcgt tgttagtggt actgcgtaac tgcgatccat
    11341 tgctctatca atatgttgat ggagggggct tttttacctc acatggccaa cccaacaaac
    11401 aggtgcgtct cgtttggtat atctatgccc aacgttatcg cgatcatcac gatgatgaat
    11461 ttattcgccg ctgtgagcgg gtgcgtcggc agtttattct gaccagcgca ttgtgtggtc
    11521 tggtggtggt cagcctgatt gcattgatga tttggcatta agcacttcag caataataac
    11581 gtataaaaaa agcgggccag taaactgacc cgctcgagac tgattacaaa cgcaaaattg
    11641 cctgatgcgc tacgcttatc aggcctacat aatctctgca atctattgaa tttgcatgtt
    11701 tttgtaggcc ggataaggcg ttcacgccgc atccggcatg aacaaagcgc actatgtcaa
    11761 caatctgaag cgggccagct gacccgctcg ttttggtgtg tacgattaca ggaactgcaa
    11821 ggagagccag tacagcccgc cggaaagcag taccgcagcc ggaagggtaa acacccaggc
    11881 catcagaata ctggttacgg ttttacgctg taagccgccg ccatctacca ccatcgtccc
    11941 cgcgacagaa gaggagagta cgtgagtagt ggaaaccggc atcccggtat aactcgccag
    12001 gccgatagac actgccgccg tcatctgggc agacatcccc tgagcgtagg tcatgccttt
    12061 cttaccgatt ttctcaccga tagtcgttgc cacacgacgc cagccaatca tcgtaccgat
    12121 acctaacgcc agcgcgaccg ccatgatgat ccacaccggt gcatactcga tggtgctaag
    12181 catgtcggac ttcagtttct tcaacaggcg ctgatcgtca gcactcacgc caggcatctt
    12241 caccactttg tcgatagtgt cagaaacgca cagcataatg cggcgcatct ggctacgttg
    12301 atcaagcgac agcttgtcgt agctttccac atcggtagtc aacatgcctt tcaggcggtt
    12361 gagcgcgtta atggtattcg acggatggca atggaactcc gcgggttgcg ttgcgccagc
    12421 ttccggagcc ggtactaact gatcagcacc ggtagcctgt ttcagcagcg caggatgctg
    12481 ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta cgggtgattt cgtagccagt
    12541 ggcattcata ttcaccacga agcctgctgg tgcgacgcca atcaatacca acataaccag
    12601 accaatgcct ttctgaccat cgttcgcgcc gtgcgaaaac gccacgccga tagcggaaag
    12661 gatcagcgca atacgcgtcc agaacggcgg ctttttcttg ccgtctttct tttcacgctc
    12721 cgctggggtc aggtggatac gggcgcgttt cttggtgccg ctccagtagc gacgcagcaa
    12781 gaaaatcaga ccgccagcaa acaccaggcc gacaataggg gaaacgatca gagaaccgaa
    12841 aatacttaat actttcggga tattgagtgc atccaccact gacgtcccgg tcatcaacgc
    12901 attggttaaa ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg atgcaggtaa
    12961 accaaagtac caggtaccca ggttccagat aatcgccgcc agcaacatag agaacaccat
    13021 ggcaaggcca tgagacgatc ccatattaag cagcagatcc gtcggcagca tatgcacaat
    13081 ggcataggca acactcagac cacccagcaa aacacccaaa aagttgaaca ccgccgccat
    13141 aaccacggcg agctgagaac gcatcgcgcg ggtatagata acggttgcca cggcgttggc
    13201 tgtgtcatgg aaaccattga tggcttcgta gaacagcaca aaagccagtg caagcaataa
    13261 taacagcccg gtatgcaaat ccaggccagc aaacaaatgt agcataggac gttacgccat
    13321 tttgaggaca tgaacgcggc gcattatcag tgactttcgc ggcgcgggca aagtgaaata
    13381 tagacttttt ttgatttgcc tcctgtatgg att
//
LOCUS       DAPHBJ010000117        13374 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.118, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000117 DAPHBJ010000000
VERSION     DAPHBJ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.118"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..253)
                     /locus_tag="RHZ76_003013"
     CDS             complement(<1..253)
                     /locus_tag="RHZ76_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813612.1"
                     /translation="MLVNSWCNALNEAISTSATICAEDLYKGGHWATAKAILDDYPID
                     LWILSAGLGLLHHKDNVVPYKATFAVGYDESIPLYSQEYV"
     gene            complement(794..2038)
                     /locus_tag="RHZ76_003014"
     CDS             complement(794..2038)
                     /locus_tag="RHZ76_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV2813613.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGSGMHLLVHPNGSKYWRLQYRF
                     DGKQKMLALGVYPDVSLADARTRRDDARNLLANNIDPGDKKKSDKIEQEEARTFEQLA
                     VEWHATNKKWSEEHSRRVLKSLEDNLFPAIGKRNIADLKTRDLLAPIKAVELSGRLEV
                     ASRLQQRTTAIMRYAVQSGLLDYNPAQEMVGAVASSNRQHRPALQLKRIPELLRRIDS
                     YTGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETSMWTIPAEREAIDGVKHSQRGS
                     KMRTPHLVPLSHQALAILKDVYKLSGDRDFVFIGDHDHRKPMSENTVNKALRVMGYDT
                     KVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHMERNSVRAAYIHKAEHLDERRLML
                     QWWADFLDANRESEVSPYDFGK"
     gene            complement(2225..2309)
                     /locus_tag="RHZ76_003015"
     tRNA            complement(2225..2309)
                     /locus_tag="RHZ76_003015"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2273..2275),aa:Leu,seq:caa)
     gene            2505..3524
                     /gene="ahr"
                     /locus_tag="RHZ76_003016"
     CDS             2505..3524
                     /gene="ahr"
                     /locus_tag="RHZ76_003016"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HDV2813614.1"
                     /translation="MSMIRSYAAKEAGGELEVYEYDPGELRPQDIEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLIAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            3654..5156
                     /locus_tag="RHZ76_003017"
     CDS             3654..5156
                     /locus_tag="RHZ76_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HDV2813615.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGQLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDVKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(5275..6357)
                     /gene="lptG"
                     /locus_tag="RHZ76_003018"
     CDS             complement(5275..6357)
                     /gene="lptG"
                     /locus_tag="RHZ76_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDV2813616.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(6357..7457)
                     /gene="lptF"
                     /locus_tag="RHZ76_003019"
     CDS             complement(6357..7457)
                     /gene="lptF"
                     /locus_tag="RHZ76_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDV2813617.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMCTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            7724..9235
                     /gene="pepA"
                     /locus_tag="RHZ76_003020"
     CDS             7724..9235
                     /gene="pepA"
                     /locus_tag="RHZ76_003020"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDV2813618.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            9589..10032
                     /gene="holC"
                     /locus_tag="RHZ76_003021"
     CDS             9589..10032
                     /gene="holC"
                     /locus_tag="RHZ76_003021"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HDV2813619.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            10032..12887
                     /gene="valS"
                     /locus_tag="RHZ76_003022"
     CDS             10032..12887
                     /gene="valS"
                     /locus_tag="RHZ76_003022"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDV2813620.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(12941..13374)
                     /locus_tag="RHZ76_003023"
                     /pseudo
     CDS             complement(12941..13374)
                     /locus_tag="RHZ76_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
BASE COUNT         3343 a         3422 c         3549 g         3060 t
ORIGIN      
        1 ctacatattc ttgagaatat aatggtattg attcgtcata tccaacagca aaggtcgctt
       61 tataaggtac aacattgtct ttgtggtgaa ggagtcctaa accagcagaa agaatccata
      121 aatcaatagg ataatcatct aagatggctt tagccgtcgc ccagtgccca cctttataaa
      181 gatcctctgc acatatagtt gcagatgtag aaatagcttc attcagagca ttgcaccaag
      241 aattcacgag cacatttgaa ggggttcggc ctgatgaaaa ttgactcaga ctcaatatat
      301 ccctactacc gtagtgtttg ccgtttgtac atgtggttat taagttgatg ttgctcacaa
      361 tcgccccccc agaatcgtgt tacacaagct acaggagaaa aaaggggatg acaagagcgg
      421 agtaatcagt ccctgaaaat tttttcaggg actgatgtta gtgaggaata tactcgaaat
      481 actcatcttg agaatgagaa catctttggc atcgcctcag aatacctttt tcctcaagag
      541 agagacaaaa atttcgacac gactcaagaa ggtcgtcagg attttcattt ttatctttag
      601 aaatcaacgc tctagctagg gtgtcataac taaaacctcc cgttgagtgc gatacaatgc
      661 tcatcgcaag ttgagtatcg aaagttttca tagtgcacct ctgagtcgcc agaataatac
      721 agttataaaa aagtataggt tatagttcaa gccggctttt caaaatcgaa catagcaagc
      781 atcaggaatt agactatttg ccaaagtcat aagggctaac ttcgctttcc ctattcgcat
      841 ccaaaaaatc agcccaccac tgcagcatca gtcgccgttc atcaagatgt tcggccttat
      901 gaatgtaagc tgcgcgtact gagttacgtt ccatatggct catctgcctt tccactgcat
      961 ccctcgacca caatccagac tcgatcaatg aactacaggc cattgtcctg aaaccgtgcc
     1021 cacaaacctc taccttcgtg tcataaccca taacgcgcag cgccttgttc accgtgtttt
     1081 cactcatcgg tttgcgatgg tcatgatcgc cgatgaaaac aaaatcgcga tcaccactaa
     1141 gtttgtaaac atctttaaga attgctaaag cttgatgaga aagcggcacc agatgaggcg
     1201 tacgcatttt tgaaccacgc tgtgaatgct ttactccgtc aatagcttcg cgctctgcag
     1261 gtattgtcca cattgatgtt tcgaaatcga tctcagacca acgagcaaag cgtagttcac
     1321 ttgaacgaat gaataccagg agcgtgagtt cgacggcaag tctagtaaga ggtctaccgg
     1381 tataactatc tatgcgccgt aatagttctg ggattcgctt caactgaaga gcaggtcgat
     1441 gctgtctgtt acttgaagca acagcgccaa ccatctcctg cgcggggtta tagtccagta
     1501 aaccactctg cacggcatag cgcataattg ccgtggttcg ctgttgtaat cgtgaagcaa
     1561 cctcaagccg tcctgacagt tcaacggctt tgattggggc tagcaggtct cgggttttga
     1621 gatccgcaat attccgctta ccaatagctg gaaacagatt atcctccagg cttttcagta
     1681 ctcgcctgct atgttcttcc gaccactttt tatttgttgc atgccactca actgcgagtt
     1741 gttcaaaagt gcgggcttct tcttgctcaa ttttatcact tttctttttg tctcccgggt
     1801 cgatgttgtt tgccaacaaa ttacgagcgt cgtcacggcg agtccgtgca tcggcaagcg
     1861 acacgtcagg ataaacacct aaagccagca tcttttgctt gccgtcaaaa cggtactgca
     1921 aacgccagta cttagaacca ttagggtgga caagaagatg cataccactg ccatcagtta
     1981 acttgtattg cttatctgtt ggcttagctg ttctgacttt gatatctgtt agagccatgc
     2041 ttatcccctc cctgttggta caagcattat cgaaccggaa ataccatcat ctgtaccaac
     2101 atctgttggt agatgtacgt tgaagtcggt tgaccttgag agagttaata tagcgggaat
     2161 gatgggtaaa tactggattt caggcacaaa aaaagacgtc cgttgacgtc tattgatgct
     2221 ccgatggtgc cgaaggccgg actcgaaccg gcacgtattt ctacggttga ttttgaatca
     2281 actgcgtcta ccgatttcgc cacttcggca ctgaagaggt atgcggaaaa cgttgtggat
     2341 tatacctgtc gcacgccacc atgcaagcgc cagcttgctc gcacctcgct aagtgctgaa
     2401 attttcagca ttgcatacag cgatgtgtaa cctttgtcac actccaggca ccccgccctg
     2461 ccatgctcta cacttcccag acaacaccag agaaggacca aaaaatgtcg atgataagaa
     2521 gctatgccgc aaaagaagcg ggcggcgaac tggaagttta tgagtacgat cccggtgagc
     2581 tgaggccaca agatattgaa gtgcaggtgg attattgcgg gatctgccat tccgatctat
     2641 cgatgatcga caatgaatgg ggattttcac aatatccgct gattgccggg catgaggtga
     2701 ttggtcgcgt agtggcgctc gggagcgccg cacaggataa aggtttgcag gtcggtcagc
     2761 gtgtcgggat tggctggacg gcgcgtagct gtggtcactg cgacgcctgt attagtggta
     2821 atcagatcaa ctgtgagcaa ggtgcggtac caacaattat gaatcgcggt ggctttgccg
     2881 agaagttgcg tgcggactgg caatgggtga ttccactgcc agaaaatatt gatatcgagt
     2941 ccgccgggcc gctgttgtgc ggcggtatca cggtctttaa accactgttg atgcaccata
     3001 tcactgctac cagccgcgtt ggggtaattg gtattggcgg gctggggcat atcgctataa
     3061 aacttctgca cgcaatggga tgcgaggtga cggcctttag ttctaatccg gcgaaagagc
     3121 aggaagtact ggcgatgggt gccgataaag tggtgaatag ccgcgatccg caggcactga
     3181 aaacacttgc ggggcagttc gatctcatta tcaacaccgt caacgtcagc ctcgactggc
     3241 agccttattt tgaggcgctg acgtatggcg gtaatttcca tacggtcggt gcggttctca
     3301 cgccgctgtc tgttccggcc tttacgttaa ttgcgggcga ccgcagtgtc tctggctctg
     3361 ctaccggcac gccttatgag ttgcgtaagc tgatgcgctt tgccgcccgc agcaaggttg
     3421 cgccgaccac cgaactgttc ccgatgtcga aaattaacga tgccatcaag catgtgcgcg
     3481 acggtaaggc gcgttaccgc gtggttctga aagccgactt ctgatccctt tgccgggtgc
     3541 ctgtcgcccg gcacttaaat ctctttggaa ggcatctcgc aatctgctga atttagcggt
     3601 gaagagtgcg cagccgttgc ctatactcca ggcaggaaac tggaggatat ctcatgagcg
     3661 aacccctgtt aattgcccgc acgccggaca cagaactgtt tttattgccg ggaatggcta
     3721 accgtcacgg gctgattact ggcgcaacgg ggacgggtaa aactgtcacg ttgcaaaaac
     3781 tggcagagtc attgtcggaa atcggtgttc cggtgtttat ggccgatgtg aaaggcgatc
     3841 tgaccggcat cgcgcaggca ggaacggcgt cggaaaaact gctcgcaaga ctgaaaaata
     3901 tcggcgtcaa cgactggcaa ccgcatgcca atccggtggt ggtgtgggat atctttggcg
     3961 agaaaggcca tccggtgcgg gcgacggttt cggatctggg gcagctgttg ctggcacgac
     4021 tgttgaatct caacgatgtg caatctggcg tgctgaatat tatcttccgc attgcggacg
     4081 atcagggatt gttgctgctc gactttaaag atctgcgggc gattacccag tacatcggcg
     4141 ataacgccaa atctttccag aatcagtacg gtaatatcag tagcgcatcg gttggtgcca
     4201 tccagcgcgg actgttgtcg ctggaacagc aaggcgcagc acacttcttt ggcgagccga
     4261 tgctggatat caaagactgg atgcgcaccg ataccaacgg taaaggcgtt atcaatatcc
     4321 tcagcgccga gaagctttat cagatgccga aactgtacgc cgccagcctg ctgtggatgc
     4381 tttcagagtt gtatgaacaa ttgccggaag cgggcgatct ggagaaacca aaactggtgt
     4441 ttttcttcga cgaggcacat ctgctgttta acgatgcacc gcaggtactg ctggataaga
     4501 ttgagcaggt gatacggctt attcgctcaa aaggcgtggg cgtctggttc gtttcgcaaa
     4561 acccgtctga tattccggat aatgtgctcg ggcagctcgg taatcgcgtt caacacgctt
     4621 tgcgtgcttt tacgcccaaa gatcagaaag cggtaaaagc tgcggcgcaa accatgcggg
     4681 ccaatccggc atttgatacc gaaaaggcaa tccaggaact ggggaccggc gaggcgttaa
     4741 tctcgtttct tgatgtgaaa ggaagtcctt cagtggtgga acgggcgatg gtgattgcgc
     4801 cttgttcgcg gatgggaccg gtgacggaag atgagcgtaa tggcttgatt aatcactctc
     4861 cggtgtatgg caagtacgag gatgaggtgg accgggaatc cgcctatgag atgttgcaaa
     4921 aaggctttca ggccagtacc gagcagcaaa ataatccccc cgcgaaaggg aaagaggtag
     4981 cggtggatga cggcattctt ggtggattga aggatatttt gttcggcact accggaccac
     5041 gcggcggtaa gaaagatggt gtggtgcaaa caatggcgaa aagcgccgcc cgccaggtga
     5101 cgaatcagat tgtacgcggg atgttgggga gtttgctggg ggggagaaga aggtaagtca
     5161 ggtgcggtct gtgcccctca ccctaaccct ctccccaaag gggcgagggg accgatcgaa
     5221 ctcgtatttg actttatcaa caatgtgaaa gggagctttc gctcccttgc ttggttacga
     5281 ttttctcatt aacagccaca ggctgattaa gaagaagctg gcgcttggca acagtgcgcc
     5341 gatgatcggc gggatgccat aaaccaacgt cagcgggccg aagatctggt ccagtacgta
     5401 gaagacaaaa ccgaagctga taccggtgac cacacgcacg cccatcggta cgctacgcag
     5461 tgggccaaag atgaacgaca gcgccatcag catcatcacc gccacggata gcggctggaa
     5521 gattttgctc cacatgttga gctgataacg tccggcatcc tgaccgctcg acttcagata
     5581 cttcacgtag ttgtgcaaac cgctgattga gagtgcatcc gggtccagcg ccaccacgcc
     5641 cagtttgtct ggcgtgaggt tggttttcca ggtgccgctc accgtctgcg aaccggtaat
     5701 ctgtttcgga ttggtcagat cagattcatc aacctgcgac agacgccaga ctttatgttc
     5761 cgggtcaaac ttcgcagtag cggcatagcg tacggattgc agacgacgat tttcgttaaa
     5821 ggcataaatg ctgatgccac ctaactcttc gtcaccttta acccgctcaa tgtagacgaa
     5881 gttgttgcca tctttcgccc ataagccttg ctgggtagag agcaacgagc cgccgtacat
     5941 cgcctgcgca cggtagttac gcgccatctg ctcgccctgc ggcgcgaccc attcgccaat
     6001 cgccatcgtc agcaagacca gcggaatggc ggttttcatc accgacagcg ccacctgcat
     6061 acgggtaaaa ccagaagcct gcatcaccac cagttcgctg cgctgcgcca gcatcccaag
     6121 accaagcaac gccccaagca gagccgccat cgggaagaag atttgcacat ctttcggcac
     6181 gctcagcaag gtatacattc ctgcgcctaa cgcgtcgtaa ctcccctgcc cggctttttt
     6241 caactgatcg acaaatttga taatgcccga cagcgacacc agcatgaaca gcgtcatcat
     6301 gatggtggtg aaaatagttt taccgatata gcggtcaagt acgccaaaag gttgcatcac
     6361 accgctcctt tacgcgaaaa actggcgcgc aggcggcgga ccggcacggt gtcccaaagg
     6421 ttgagaacaa tcgccaaagc cagataaatc aggttaacgg tccacatcca cagcgtcggg
     6481 tccagcttac ctttaccgcc gttcgatttc agggaggtct ggatcaggaa gaaaagtaga
     6541 tacagcagca tggctggcag catcgacagt acgcgtccct gacgtgggtt aaccacgctc
     6601 agcggtacga ccataagtgc catcataaac acggtgaata ccaacgtgat acgccagttc
     6661 agttctgcgc gagcacgatc ggtgtcagtg ttccacaatg tgcacatgtc catctggtcg
     6721 gtatcgttcg ggtcgagcgc caccgcctgg tgaccaatga tcgcctgata atcctggaaa
     6781 tccgtaatgc ggaaatcacg taacagtgca gtgccttcga agcgcgttcc ctggttgaga
     6841 gtgacgacct gggagccgtc gcgcagctgg gttaaatgtc cggaatcggc caccaccaca
     6901 gaaggacgtg cattaccttt tggtcgaatt tgtgcgagga acacatcttt gaaatcgctg
     6961 ccgtcaacgc tttcgatgaa cagcaccgag ctgccattag tcgcttgctg gaattgccct
     7021 tgcgccagcg ccgccatgcc agggttcgct ttcgcttctg ctaacacttc atcctgatga
     7081 cgcgatgacc acggtcccgc ccacatcacg ttaaccgccg cgacgattgc cgtgaatacc
     7141 gcaaggatca ttgccgcttt caccagaacc gctttgctca ggccgcaggc atgcattacc
     7201 gtaatttcac tttcggtata cagtttgccc agcgtcatca gcagcccgag gaacaggctt
     7261 aatggcagga taagctgcgc catttccggc acgcccaacc cgagaaggga gagcaccaga
     7321 ttcgccggaa tatcgccgtc aaccgctgcg ccgaggatcc tcactaactt ttgacagaag
     7381 aagatcaaaa gcaagatgaa gagtatcgcc agctggcttt tgagcgtctc ccgcaccaga
     7441 tatcttatga ttatcacttt aaatacgccc gtaaaaactc gtcttttgca ggattttagc
     7501 ttgtttcatg gcttaaacgt catttattct cttgagtcgt cgaaatcgtc gctaagataa
     7561 ttatactcaa cggattcacc tctcagattt tgttctgacg tgccaatgcc gtaataacgt
     7621 taagattaac acgaagtcat cgcaacagcg gacatgagtt acgaaagctt gcaattctat
     7681 ctgtagccac cgccgttgtc tttaagattc aggagcgtag tgcatggagt ttagtgtaaa
     7741 aagcggtagc ccggagaaac agcggagtgc ctgcatcgtc gtgggcgtct tcgaaccacg
     7801 tcgcctttct ccgattgcag aacagctcga taaaatcagc gatgggtaca tcagcgccct
     7861 gctacgtcgg ggcgaactgg aaggaaaacc ggggcagaca ttgttgctgc accatgttcc
     7921 gaatgtactt tccgagcgaa ttctccttat tggttgcggc aaagaacgtg agctggatga
     7981 gcgtcagtac aagcaggtta ttcagaaaac cattaatacg ctgaatgata ctggctcaat
     8041 ggaagcggtc tgctttctga ctgaactgca cgttaaaggc cgtaacaact actggaaagt
     8101 gcgtcaggct gtcgagacgg caaaagaaac gctctacagt ttcgatcagc tgaaaacgaa
     8161 caagagcgaa ccgcgtcgtc cgctgcgtaa aatggtgttc aacgtgccga cccgccgtga
     8221 actgaccagc ggtgagcgcg cgatccagca cggtctggcg attgccgccg ggattaaagc
     8281 agcaaaggat cttggcaata tgccgccgaa tatctgtaac gccgcttacc tcgcttcaca
     8341 agcgcgccag ctggctgaca gctacagcaa gaatgttatc acccgcgtta tcggcgaaca
     8401 gcagatgaaa gagctgggga tgcattccta tctggcggtc ggtcagggtt cgcagaacga
     8461 atcgctgatg tcggtgattg agtacaaagg caacgcgtcg gaagatgcac gcccaatcgt
     8521 gctggtgggt aaaggtttaa ccttcgactc cggcggtatc tcgatcaagc cttcagaagg
     8581 catggatgag atgaagtacg atatgtgcgg tgcggcagcg gtttacggtg tgatgcgtat
     8641 ggtcgcggag ctacaactgc cgattaacgt tatcggcgtg ttggcaggct gcgaaaacat
     8701 gcctggcgga cgagcctatc gtccgggcga tgtgttaacc accatgtccg gtcaaaccgt
     8761 tgaagtgctg aatactgacg ctgaaggccg cctggtactg tgcgacgtgt taacttacgt
     8821 tgagcgtttt gagccggaag cggtgattga cgtggcgacg ctgaccggtg cctgcgtgat
     8881 cgcgctgggt catcacatta ccggtctgat ggcgaaccat aatccgctgg cccatgaact
     8941 gattgccgcg tctgaacaat ccggtgaccg cgcatggcgc ttaccgctgg gtgacgagta
     9001 tcaggaacag ctggagtcca attttgccga tatggcgaac attggcggtc gtcctggtgg
     9061 ggcgattacc gcaggttgct tcctgtcgcg ctttacccgt aagtacaact gggcgcacct
     9121 ggatatcgcc ggaaccgcct ggcgttctgg taaagcaaaa ggcgcaaccg gtcgtccggt
     9181 agcgttgctg gcacagttcc tgttgaaccg cgctgggttt aacggcgaag agtaattgcg
     9241 tcaggcaagg ctgttattgc cggatgcggc gtgaacgcct tatccgacct acacagcact
     9301 gaactcgtag gcctgataag acgcgcaagc gtcgcatcag gcaatgctgt tattgtcgga
     9361 tgcggcatga acgccttatc cgacctacac agcaccgaac tcgtaggcct gataagacgc
     9421 ggcagcgtcg catcaggcaa tgctgttatt gtcggatgcg gcgtgagcgc cttatccgac
     9481 ctacacacag cactgaactc gtaggcctga taagacacaa cagcgtcgca tcaggcgctg
     9541 cggtgtatac ctgatgcgta tttaaatcca ccacaagaag ccccatatat gaaaaacgcg
     9601 acgttctacc ttctggacaa tgacaccacc gtcgatggct taagcgccgt tgagcaactg
     9661 gtgtgtgaaa ttgccgcaga acgttggcgc agcggtaagc gcgtgctcat cgcctgtgaa
     9721 gatgaaaagc aggcttaccg gctggatgaa gccctgtggg cgcgtccggc agaaagcttt
     9781 gttccgcata atttagcggg agaaggaccg cgcggcggtg cgccggtgga gatcgcctgg
     9841 ccgcaaaaac gcagcagcag cccgcgcgat atattgatta gtctgcgaac aagctttgca
     9901 gattttgcca ccgctttcac agaagtggta gacttcgttc cttatgaaga ttctctgaaa
     9961 caactggcgc gcgaacgcta taaagcctac cgcgtggctg gtttcaacct gaatacggca
    10021 acctggaaat aatggaaaag acatataacc cacaagatat cgaacagccg ctttacgagc
    10081 actgggaaaa gcagggctac tttaagccta atggcgatga aagccaggaa agtttctgca
    10141 tcatgatccc gccgccgaac gtcaccggca gtttgcatat gggtcacgcc ttccagcaaa
    10201 ccatcatgga taccatgatc cgctatcagc gcatgcaggg caaaaacacc ctgtggcagg
    10261 tcggtactga ccacgccggg atcgctaccc agatggtcgt tgagcgcaag attgccgcag
    10321 aagaaggtaa aacccgtcac gactacggcc gcgaagcttt catcgacaaa atttgggaat
    10381 ggaaagcgga atccggcggc accattaccc gtcagatgcg ccgtctcggc aactccgtgg
    10441 actgggagcg tgaacgcttc acgatggacg aaggcctgtc caatgcggtg aaagaagttt
    10501 tcgttcgtct gtataaagaa gacctgattt accgtggcaa acgcctggta aactgggacc
    10561 cgaaactgcg caccgctatc tctgacctgg aagtggaaaa ccgcgaatcg aaaggttcga
    10621 tgtggcacat ccgctatccg ctggctgacg gtgcgaaaac cgcagacggt aaagattacc
    10681 tggtggttgc gactacccgt ccggaaaccc tgctgggcga taccggtgta gccgttaacc
    10741 cggaagatcc gcgttacaaa gacctgattg gcaaatatgt cattctaccg ctggttaacc
    10801 gtcgtattcc gatcgttggc gacgaacacg ccgacatgga aaaaggcacc ggctgcgtga
    10861 agatcacccc ggcgcacgac tttaacgact atgaagtcgg taaacgtcac gccctgccga
    10921 tgatcaacat cctgaccttt gacggcgata tccgtgaaag cgcccaggtg ttcgatacca
    10981 aaggtaacga atctgacgtt tattccagcg agatcccggc agaattccag aaactggagc
    11041 gttttgccgc acgtaaagca gtcgttgctg cggttgacgc actcggcctg ctggaagaaa
    11101 ttaaaccgca cgacctgacc gttccttacg gcgaccgtgg cggcgtagtt atcgaaccaa
    11161 tgctgaccga ccagtggtac gtgcgtgccg atgtactggc gaaaccggcg gttgaagcgg
    11221 ttgagaacgg cgacattcag ttcgtaccga agcagtacga aaacatgtat ttctcctgga
    11281 tgcgcgatat tcaggactgg tgtatctctc gtcagctgtg gtggggtcac cgtatcccgg
    11341 catggtatga cgaagcgggt aatgtttatg ttggccgcaa cgaagaagaa gtgcgcaagg
    11401 aaaataacct cggtgccgac gttgccctgc gtcaggacga agacgttctc gacacctggt
    11461 tctcttctgc gctgtggacc ttctctaccc ttggctggcc ggaaaacacc gacgccctgc
    11521 gtcagttcca cccaaccagc gtgatggtat ccggcttcga catcatcttc ttctggattg
    11581 cccgcatgat catgatgacc atgcacttca tcaaagatga aaatggcaaa ccgcaggtgc
    11641 cgttccacac tgtttacatg accggtctga ttcgtgacga cgaaggccag aaaatgtcca
    11701 aatccaaggg taacgttatc gacccgctgg atatggttga cggtatttcg ctgccagaac
    11761 tgctggaaaa acgtaccggc aatatgatgc agccgcagct ggcggacaaa atccgtaagc
    11821 gcaccgagaa acagttcccg aacggcattg agccgcacgg caccgatgcc ctgcgcttca
    11881 ccctggcggc gctggcgtct accggtcgtg acatcaactg ggatatgaag cgcctggaag
    11941 gttaccgtaa cttctgtaac aagctgtgga acgccagccg ctttgtgctg atgaacacag
    12001 aaggtcagga ttgcggcttt aacggcggcg aaatgacact gtcgctggcg gaccgctgga
    12061 ttctggcaga gttcaaccag accatcaaag cgtaccgcga agcgctggac agcttccgct
    12121 tcgatatcgc tgcaggcatt ctgtatgagt tcacctggaa ccagttctgt gactggtatc
    12181 tggagctgac caagccggta atgaacggtg gcaccgaagc cgaactgcgc ggtactcgcc
    12241 atacgctggt gactgtactg gaaggtctgc tgcgcctcgc gcatccgatc attccgttca
    12301 tcaccgaaac catctggcag cgtgtgaaag tactttgcgg tatcactgcc gacaccatca
    12361 tgctgcagcc gttcccgcag tacgatgcat ctcaggttga tgaagccgca ctggccgaca
    12421 ccgaatggct gaaacaggcg atcgttgcgg tacgtaacat ccgtgcagaa atgaacatcg
    12481 cgccgggcaa accgctggag ctgctgctgc gtggttgcag cgcggatgca gaacgtcgcg
    12541 taaatgaaaa ccgtggcttc ctgcaaaccc tggcgcgtct ggaaagcatc accgtgctgc
    12601 ctgccgatga caaaggtccg gtttccgtta cgaagatcat cgacggcgca gagctgctga
    12661 tcccgatggc tggcctcatc aacaaagaag atgagctggc gcgtctggcg aaagaagtgg
    12721 cgaagatcga aggcgaaatc agccgtatcg agaacaagct ggcgaatgaa ggctttgtcg
    12781 cccgcgcacc ggaagcggtc atcgcgaaag agcgtgagaa gctggaaggc tatgcggaag
    12841 cgaaagcgaa gctgattgaa cagcaggctg ttatcgccgc gctgtaatca acaccgtgat
    12901 gaaaacgaag gccggagaat gctccggcct tttttatctc ttacagaaat ggtaaagaag
    12961 gcataattcc ccgtgaaata ctggctaaga agcctttatc tggttgttct tctttattga
    13021 ttaaaggaag gtcatccaaa tcgcccagca aatacgtatt ttttgcctgc cagtcaatca
    13081 tccatatttt cagcagtgga taagccagac cgcccgtaat cccggatatc accaccaacg
    13141 cacacatgcg ataaagcata ccgtggtatg ttaaagttga gcgaaaacga atacgcccat
    13201 cattcagtga caggttgctc ataaaatgat ttcgcaacga caccgttaaa taactggtgc
    13261 taacagcaat cccaaaataa tagatttact gcgcgattat cattttacgt tgcatttcca
    13321 taaattgctg taaattctca atatcatcat ttgcatacac agaactatca tacg
//
LOCUS       DAPHBJ010000118        13311 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.119, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000118 DAPHBJ010000000
VERSION     DAPHBJ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.119"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            3..827
                     /locus_tag="RHZ76_003024"
     CDS             3..827
                     /locus_tag="RHZ76_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HDV2813621.1"
                     /translation="MPLSNTLQSQIITDNHFLHHPKVDSELTRKYERARLDTENIYLL
                     PLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKITS
                     ALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYAGT
                     TIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGNDP
                     IIGIEAYPGTAEIHAKMGYKVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK"
     gene            complement(968..1741)
                     /gene="uxuR"
                     /locus_tag="RHZ76_003025"
     CDS             complement(968..1741)
                     /gene="uxuR"
                     /locus_tag="RHZ76_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HDV2813622.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSSDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDV"
     gene            complement(1956..3416)
                     /locus_tag="RHZ76_003026"
     CDS             complement(1956..3416)
                     /locus_tag="RHZ76_003026"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HDV2813623.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3497..4681)
                     /gene="uxuA"
                     /locus_tag="RHZ76_003027"
     CDS             complement(3497..4681)
                     /gene="uxuA"
                     /locus_tag="RHZ76_003027"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDV2813624.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5021..6364
                     /gene="gntP"
                     /locus_tag="RHZ76_003028"
     CDS             5021..6364
                     /gene="gntP"
                     /locus_tag="RHZ76_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HDV2813625.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6537..7439)
                     /gene="fimH"
                     /locus_tag="RHZ76_003029"
     CDS             complement(6537..7439)
                     /gene="fimH"
                     /locus_tag="RHZ76_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HDV2813626.1"
                     /translation="MKRVINLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVVIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIAVTFVYQ"
     gene            complement(7459..7962)
                     /gene="fimG"
                     /locus_tag="RHZ76_003030"
     CDS             complement(7459..7962)
                     /gene="fimG"
                     /locus_tag="RHZ76_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HDV2813627.1"
                     /translation="MKWCKRGYVLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNSGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7975..8505)
                     /gene="fimF"
                     /locus_tag="RHZ76_003031"
     CDS             complement(7975..8505)
                     /gene="fimF"
                     /locus_tag="RHZ76_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HDV2813628.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAVAGLGIQLLNEQQNQIPINAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8515..11151)
                     /gene="fimD"
                     /locus_tag="RHZ76_003032"
     CDS             complement(8515..11151)
                     /gene="fimD"
                     /locus_tag="RHZ76_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HDV2813629.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAEQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATCDVTFNTGDSE
                     QGIVPCLTRAQLASMELNTASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLVGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNDNI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11218..11943)
                     /gene="fimC"
                     /locus_tag="RHZ76_003033"
     CDS             complement(11218..11943)
                     /gene="fimC"
                     /locus_tag="RHZ76_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDV2813630.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11980..12519)
                     /locus_tag="RHZ76_003034"
     CDS             complement(11980..12519)
                     /locus_tag="RHZ76_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV2813631.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12584..13138)
                     /gene="fimA"
                     /locus_tag="RHZ76_003035"
     CDS             complement(12584..13138)
                     /gene="fimA"
                     /locus_tag="RHZ76_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDV2813632.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAETTPTTVNGGTVHFKGEVVNA
                     ACAVDAGSVDQTVQLGQVRTASLKQTGATSSAVGFNIQLNDCDTSVATKAAVAFLGTA
                     IDSAHPKVLALQSSAAGSATNVGVQILDRTGNELTLDGATFSAQTTLNNGTNTIPFQA
                     RYYAIGEATPGAANADATFKVQYQ"
BASE COUNT         3226 a         3467 c         3204 g         3414 t
ORIGIN      
        1 ctatgccatt aagcaatacc cttcagagcc aaattattac agataatcac ttccttcatc
       61 atccaaaagt cgacagtgag ttaacacgta aatatgaacg tgcacgcctg gatacggaaa
      121 atatctatct tctgcctctt gctcgtggca ataaccataa ttacgatgga aaaagcgttg
      181 tcgaaattag aaaacttgat attagtaaag agtcctggcc atttaattat gtgacagaag
      241 catgcagaga atccgatggt attacaacaa caggacgtat gctctatcga aacttgaaaa
      301 tcacctcagc tttagacgaa atatatggtg gcatatgtaa aaaggcgcat gctgctacag
      361 aattagcaga aggtttacgg ctaaatttat ttatgaagag ccctttcgat cctgttgagg
      421 actatacagt tcatgaaata acattaggcc ctggttgcaa tgttccaggt tacgccggaa
      481 ccacgatagg atatatttca acccttccag tttctcaggc aaaaagatgg accaatgagc
      541 agccaagaat tgatatctat attgatcaga taataactgt ctcaggggta gcaaattctt
      601 ccggattcgc ccttgccgca ttacttaatg ctaatattga gatgggaaat gacccaatca
      661 ttggtataga agcctatcct ggtaccgcgg agatccatgc aaaaatggga tataaagtta
      721 tccccggtga tgaagatgcg ccactcaaac gcatgactct gcaaccgtca tctcttccag
      781 aactgtttga attaaaaaat ggtgaatgga attatattgg taaatagcca ctgagaagaa
      841 tgagtactaa tattcaggtt atacagatat ttaaacaata ccctgcaatt tacaaagcaa
      901 tactcattgc gacttcttaa tttaagaaag cgcaaggaac gtttaccctt gcgcttatta
      961 taataagtca gacgtcgact ttatccagcg gccatgaatc aaacagatag ccgtcaaaat
     1021 aaatatcgtc aacgttcgag aattccagca gacgttgctt aacgttttcc agatgctgcc
     1081 acattgccag cttcgccgct cgggcatctt ttttgattaa cgcggcgagg atctgtttgt
     1141 gatcgcccaa ccactctttg cgatacaggc tgtcatccag atggctgtgc aactggatcc
     1201 acattggatt gttttcccgc cactgccagg actgacggaa taactccacc agcatgctgt
     1261 tatgcgttgc ttcggcaatg gcgagatgga actgcatatc accgctttcg ctgctacccg
     1321 gcgcgctgga agccagttca cgctcttcca gttgcaatgc ctgacgcatt ttgacgatat
     1381 cttcgcgggt agcctgcaaa gcggcaaatt ccgcgatgtt actttccagt aactgacgcg
     1441 cctgtaacag ctcaaaagga cccgcatcat tacagacgtt ggcatccgaa ctgtctgtgt
     1501 tgtggctgcc tgagctatca agaacataga taccggcacc ccggcgcact tccaccagcc
     1561 ctttgatctc cagcatgatc agcgcttcac gtaccaccgt ccgcgtgaca tcaagcattt
     1621 ctgcaatttc acgctccggc ggcagccgtt cgccaggatt gtacggcgtc tttatgatca
     1681 gatcgcggat catcgccccg acttcctggt aaggtctttg cgcagaggtg gcagatttca
     1741 tggtgttgtg tccgttaggt gcggttaatc aaatttttgc cggatgcggc gtgaacgcct
     1801 tatccggcct acaacgggca attttacggc ccctgttaat ctcaaactat accattagca
     1861 ctggaagttg gcacgtacag gtgggcaaaa aaacgaaaac tggtcaacca aatatcagtt
     1921 tgattgacca gtaagtctgt agggtaatca gttagttaca gcgcagccac acactcgcgc
     1981 gcaccgcgtt cgcacagctg ctgatatgcc gccgtcaccg cgccaacaaa gtcggcattc
     2041 tgcggcagat catcggcaaa aataccgctc aggcctagca gcgctttcac gcggtctgcg
     2101 ccctgatact gcgcgttaat cttctggaac tcggccagca tcgggtcgac cacgtcaatg
     2161 gcattaccct gctcatccac gccctgggtg taacgcatcc agccagccac gcccagcgcc
     2221 aggtgacgcc agctgccgcc gttttgcagg tgcagacgca ccgggtccag cagacgctgc
     2281 ggtaacttct ggctgccgtc catcgcaatc tgccaggtac ggtgacgcag agacgggttg
     2341 ctgaaacgct cgatcagcag cgtcgcatag gcgttcaggt ccgtaccttc cggcatcgac
     2401 agcgttggcg cttgttcctg catcatcagg gcaaaggccg ctttgcgata atccgggtta
     2461 gtcatggtgt cggcaatagt ttcatagccg ccgaggtaac cgaggtacgc caggaaagag
     2521 tggctaccgt tcagcatacg cagcttcatc atttcgaacg gcacaacgtc tgcaacgaac
     2581 tgtgcgccca ctttatccca gtccgggcga ccattaacga aattatcttc aatcacccac
     2641 tgacggaacg gttcgcaggc aatggcgcac gggtcgtaaa cgcccagctg gtcagcaatt
     2701 tcctgtaagg tttctggcgt cgccgccgga acgatgcggt caaccatggt gcacgggaag
     2761 gtgacgttct cttcaatcca tgccgctagc tgcgggtcac gcgcctgagc cagccccagt
     2821 accgcgacct tcgccacatg accgttttca cgcacgttat cgcaggacat caccgtaaat
     2881 gctttcagcc ctttttcacg acgcagacgc aaggcttcga cgatgtaacc aatcgcggac
     2941 ttcggcgcag tcgggttttc cagatcgtgc ttgatcagcg ggttattgag atccagttga
     3001 ccgcttgccg catcagcgca gtagcctttt tccgtgaccg tcagagagac aatcgccgtt
     3061 tgcggacgcg ccatcgcgtt gagaatacct tcgcagccgt cgatttccgg gtgcagcgct
     3121 tctttcatcg aaccgataat tttcagctcg gtgctttccg cgcctttttc cgccacggtg
     3181 tacagcagtt gctgtttttt caggttttcg atcagcacgc ggtcgttgcc tggcatcagg
     3241 ttaacttcgc agatacccca gtcgctgtcg gtgctttcca gcagatgatg ggtgtacagc
     3301 gcctggtgcg cacggtgaaa cgccccgcaa ccgagatgca caatgcgtga ttccagacga
     3361 gaatgatccc atgacgggcg ggcaaccggc agattgctgt caacaatagt agtcatcgta
     3421 aactccagaa tccgtcttca gagagtgacg gcaaggaaga aggaaccggc acggcgcgca
     3481 gccatgccgg tggatattaa cggctaaaga aagcgcgctg gatcgccagt tcgacaccgc
     3541 gaacttcggc caggcctttc agacgaccaa ttgcggagta acctgggttg gttttcttct
     3601 tcaggtcgtc cagcatctga tgaccgtggt ccggacgcat cgggatcagg tcttctttgc
     3661 cttccgcttt acgacggtgt tcttcttcaa caatcgcttt caccacttcg tacatatcaa
     3721 cgtcaccgtt caggtgcgcc gcttcgtgga aggttttcgg gttatcttca cgcatggtgg
     3781 agcgcagatg ggtgaagtaa atacgcggac cgaactgctt gatcatatca accagatcgt
     3841 tgtcagcacg cacgccgtag gaaccggtgc acatggtgaa accgttcgcc atgctattta
     3901 cggtatcaac catccactgc atatcttcaa tggtagaaac aatgcgcggc aggccgagga
     3961 ttgggcgcgg cggatcgtcc gggtgaacag ccatacgcac gccaacttct tcggcaaccg
     4021 gaataatcgc cttcaggaag acggcaaagt tttcgcgcag tttggctttg tcgatatctt
     4081 tgtacagctc caggtgctta cggaactggt cgagggtgta cccttcttcc gcgcccggca
     4141 gaccggcaat gatgttacgg gtcagacgcg ctttgtcttc gtcgctcata gtggcgaagc
     4201 gttcagctgc ctgagcaatt tcttcttcgg tgtaatccgc ttccgcacct gggcgtttca
     4261 ggatatgcat ttcgaatgca gcgaattcga tctggtcgaa gcgcagagct ttggagccgt
     4321 ctggcagcac gtattcgagg tcagtacggg tccagtcgag caccggcatg aagttgtagc
     4381 acacggtgcg aataccgcac tgcgccaggt tgcgcagagt ctgctgatag ttagcgatcc
     4441 actgctcata gttgccagtg tgggttttga tatcttcgtg aattggcacg ctttctacga
     4501 cagaccacac caggcctgca tcttcaacga tagccttgcg tttgaggatc tcttctacgg
     4561 accatacttc accgttcggg atatggtgca aagcggtaac cacgccagtc gcgcccgcct
     4621 gacggacatc agctaaagaa accggatcgt ttgggccgta ccagcgccag gtctgttcca
     4681 tgatttttcc tctttcacat agtgggttcc acagcaaaga gacgcggaac cctggagttg
     4741 tttaggtatc cggatgaaca accagaccgc agccgtggcg ttgcatcgga tgagatggcg
     4801 tataagttct accttttggt aagacatgtc aacccaaatt ctgaaattgg ttaaccacat
     4861 cacaagaatt tcactctcct ggttgttgct gcaaattgca gcaaaaggta atcgatggtt
     4921 attgatcaga ggatgtgaca ttcatcgcaa caatggttga ccaatttaca taacatgtcg
     4981 cccaaaataa caacggttca accaggtaag gtaaaacgtt atgcatgtgc ttaacattct
     5041 ctgggtggta ttcggcattg gtctgatgct ggtactgaat ttgaagttca aaatcaattc
     5101 aatggtggcc ttgttggtgg cggcgctgtc cgtcgggatg ctggcgggca tggatttgat
     5161 gtcgctgctg cacaccatga aagcgggctt cggcaacacg ctgggggaac tggctatcat
     5221 cgtggtgttc ggtgcggtca tcggtaaatt gatggtcgac tccggcgcgg ctcaccagat
     5281 agcgcatacg ctgctggcac gtctcggtct gcgttatgta cagctgtcgg tgattatcat
     5341 cggcctgatt ttcggtctgg cgatgttcta tgaagtggcc tttatcatgt tagcaccgct
     5401 ggttattgtt attgccgccg aagctaaaat tccgttcctg aaactggcga tcccggcagt
     5461 agcagctgcc actaccgcac attcactgtt cccaccgcag ccgggtccgg tggcgctggt
     5521 gaatgcttat ggcgcggata tggggatggt ttatatctat ggcgtactgg tgacgatccc
     5581 aagtgtcatc tgcgcaggtc tgatcctgcc gaagttcctc ggcaatcttg agcgcccaac
     5641 gccatcattc ctgaaagcag atcaaccggt agatatgaat aatctgccct ctttcggcgt
     5701 ttcgattctg gtgccgttga tcccggcgat cattatgatc tctaccacca tcgccaatat
     5761 ctggctggta aaagataccc ctgcgtggga agtggttaac tttatcggtt cctcgccgat
     5821 tgcaatgttt attgcgatgg tggttgcatt cgtactcttt ggcaccgcgc gcgggcacga
     5881 tatgcagtgg gtaatgaacg cttttgaaag cgcggtgaaa agcattgcaa tggtgattct
     5941 gatcatcggt gcaggtggcg tgctgaagca gaccatcatc gacaccggca tcggcgacac
     6001 catcggcatg ctgatgtccc acggcaatat ctcgccctac atcatggcat ggctgatcac
     6061 cgtactgatt cgtctggcga cgggtcaggg tgtggtttca gcgatgaccg ccgccgggat
     6121 tatcagtgca gcaatccttg atccagcaac tggtcagctg gttggcgtga atccggcgct
     6181 gctagtactg gcgacggctg cgggttccaa caccctcacc cacattaacg atgcctcatt
     6241 ctggttgttc aaaggttact ttgacctgtc agtaaaagac acgttgaaaa cctggggtct
     6301 gctggagctg gtcaactccg tggttgggct gattattgtg ctgattatta gcatggtagc
     6361 gtaaaagagc acctgagcct gccattggca ggctctgttc ggattgtcgg taaagtgcgc
     6421 tttgtttatg ccagatgcga cgctgacgcg tcttatctgg cctacaaagg gctaacgtgc
     6481 aggttttagc ttcaggtaat attgcgtacc tgcattagca atgccctgtg atttctttat
     6541 tgataaacaa aagtcacggc aataatcgat tgcacattcc ctgcagtcac ctgccctccg
     6601 gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagtccctac tgctcctaac
     6661 gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac gccgacgccc
     6721 tgtgcaggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc tgcggttgtg
     6781 ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag aggaatcggc
     6841 actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac atcgcagccg
     6901 ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg gaaatcatcg
     6961 ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgagcc agctttaatc
     7021 accaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca cggcttatcc
     7081 gttctcgaat tataaacaac ccgcggcgtt tcactggtgg tcggaaatgg atagctactg
     7141 ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccataagc cgagcctcgt
     7201 tgcagtgtga cataatctgt aatggtttcc gggtaatcgt tatggcaaaa gatttgcgtc
     7261 gaaagatcca cgaccaggtt ttgccccaca ttcacggcgg gcgcaaggtt tacataaaca
     7321 ttggcgctgc caccgccaat agggatagcg gtgccattgg cggttttaca ggcgaatgac
     7381 caggcattta ccgaccagcc catcagcagt acagcaaaca ggttaataac tcgtttcatt
     7441 acaatcatct cttcggcttc agctgtaggt ataggtgatg ctaatcactg cctgaatggt
     7501 tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga agtgcgctga
     7561 ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca ctattcaatg tgttgccact
     7621 gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat aatatccggt
     7681 actgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg gacaattagt
     7741 caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacatca gactgaaaga
     7801 ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtgcacg gtttggcgac
     7861 gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac ttgcgagcgc
     7921 caatattgcc gccaatacat acccacgttt gcaccatttc atgagcctct ccagttactg
     7981 atattcaaga gtgaaggtag ccgtggcatt gatatgcccc gcagtgacag gcacctgtgt
     8041 cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca gggtcgtcca
     8101 cgaaatcgcg gacgatgggg cattaatggg tatctgattt tgttgctcat tcagaagctg
     8161 tattcccagt cccgcaaccg ctgacaccgt attttcaagt gcaagcaggt tggcattgtg
     8221 gctatctgca acaccggtaa acccaacttt tacggcagaa acggcattac cacagggtga
     8281 cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa attgcttcgc
     8341 cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac tacagccgtt
     8401 atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaagcgt gacttaccgc
     8461 cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc ccccttaacg
     8521 acattcagct gatagctgag ttaataactg ctgctgactc tctggtggca gttgataatt
     8581 ggcgacacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc ccgctaaagg
     8641 cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct ggctactctc
     8701 tgatgtcacc atcgccccaa acggcagcgg cttattattg tgggttagcg tcatgagcag
     8761 ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacggg tgggaacaac
     8821 gttagcgacc gcgttatcta aatcgacgtt atcagccagg gtattggtat ccagcgctac
     8881 tctgttctcc cgatattcag tggcataagg cagcacggca taaccgcgcc agtcggtacg
     8941 cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa caagcaccac
     9001 cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccagta ccccaccgct
     9061 gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatgt tgtcattgcc
     9121 gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac catcacctcc
     9181 cccggcgtaa ccggtttgca cgctatagct gaggttgttg tcttccagca acgtaccgta
     9241 tacaccgacc agattggtca tccgaccgtt gagatcgtgt gacatgctgt aactggcact
     9301 ggcatgtcgc cactgagatt tactgtcaga acgcagccag tggctgaaag gaatattgac
     9361 gttaagcgct aacatctgat cccgtccttt ttgccaggcg tttttcgtca ggctatagct
     9421 gagcgtccag ttgatatctt cgaatgcagt atttaaacca gcctggaatt gctcatcgac
     9481 attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg atgtgcgccc
     9541 gagttgctga gtaacggtga gttgtaattt cccgcgtttg ttataagcga gattgtaata
     9601 gtcggtgaat ttcggcttaa cctgaataac accatcctgt gtttcgatgt tgtagccatt
     9661 cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat aacggtaacc
     9721 cactaactga atattcgtgc ctgattcatt gagcgatttg ttatagagaa aacgcaccga
     9781 ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca tatccacaga
     9841 cagagcgccc agtgccccca tgtttttccc gataccgaaa ttaaacgcac gataacgatc
     9901 cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga gtaatgtact
     9961 ctggaaaaag cggggttttt cctgctgcgc atttccacta cggtattctc ctgccgtaat
    10021 ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg gtacggtaaa
    10081 gatctgcgtg ctgccgtcag cctctttaat cgttacctgc aagtcaccac tattacctgc
    10141 ggcatagata tcgttgatgg taaaaggccc cggcggcacc gtactattat aaatgtcata
    10201 cccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtgga tcaccggagc
    10261 aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg cgccgcgaaa
    10321 gttaatacca tcgaaaatat cgccctgggt ataaccatca cccagcgtca gccgggaacg
    10381 taacggaatt atgtctcgct caagccaggt attgatatgc tgccatttat ttttgctacc
    10441 tgatgaactg tcgctactgt tataactcca ggtggtattg tcgcgtaaac gccacgcacc
    10501 aatatttaac ccactctgta ggtttaaata tgcataatgg ctgttacccc caatccgatt
    10561 ctgtacacta tttccgctga aattataatt gagcaatccg gcattaatac caggatccca
    10621 taactcagga ggaatataac cacgcgcgcg attactcata aatgcctgag ggatcgtcag
    10681 gttgagtcgc tgctgaccaa catccagatg cgcagtagcg tcatggatca ttgaggttaa
    10741 tggcacgcag gcatcatccg ccagcagatt cataccggag acagaagccg tattcagctc
    10801 catacttgca agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac tgtcgcccgt
    10861 attaaatgtg acatcacacg ttgccatata accattattc aaatagatat cgacacgata
    10921 cgtccctggc ggtaattctt gcccattttc aaaacgcgat aaatcggcca cagcctgggg
    10981 atcatccgct aaaaagcgcg gattaaaata gagttcggca gatgacaaag gtgcctgttc
    11041 ggcaaaagca caggcgacaa agagccggac aaaaaaacca gccaaacgat gcttacgaat
    11101 atgcaagcat tgtgtgtttc gctggtaaag tcttaaattc agatatgaca ttattcctcc
    11161 ggtttaaaat cacccggcag tcaattcttt ttattcaggc gaaaaattcc ccctgcgtta
    11221 ttccattacg cccgtcattt tgggggtaag tgcgccataa tcatttattg ttcggtaagt
    11281 aatattgctt cctgcatcag aaggcaattt aaccgtgctt tcgcccattg gaggcaccaa
    11341 tgcattttca agaacccggg ttccggcatt caactctgtt accgtcaggt aatagggtgt
    11401 cgggttaatc agagtcagag aattcgcgct acgacgaaat cttaattttt ctgcggcctg
    11461 atcgggtggc aacgctaatt tagccgggcg atagtacagt ttaatgcggc tgataattgc
    11521 gagctgtagc gtattctcag tcaattttga tttatccatt gacggaatcg ctttaacgtt
    11581 catccagaat aaactttccc gatcctgtgg caattggtta tttgttgcat caagaatacg
    11641 taaggtattc tctttttttc ccttcatcgc aaacagaggt ggcgtcacga taaaacgacc
    11701 atcctttaca ccatcggcat tttccaccca tgattgaatt aaataggtgc tattttcatc
    11761 attatttgtc acggcaagtt gcacttgttt ttgccctgcc ggataaatta cgcgagtcgc
    11821 acctaaggcc actcccgctt cggcgcgtcc ggcaaccatc attgccatga acatcaggat
    11881 acctgccagc aagcagaatg ttatttcctg tgatttcctt acattgacgt ttttattact
    11941 cactgtcctg ttcctgttat cacattatct gctgaacaat tactgatagg ttaaagagaa
    12001 ccaggcctgg gcattggcga tgccgccagt aacccgacgc ccggtagcac gatatttggc
    12061 gatgaaatgt agcgaagtag agcctgagta aagccgtttc cagtttgctg gaggacgatt
    12121 aatcggtacg aggtttcctt catcatcaaa caacgctacg ccaatattgg tggctatccc
    12181 tggcccctct cccacggaaa gcacatctgg atttttacca tccgcgacac cgtgaaacgc
    12241 cacacccaca cgttcactca ccaccgtgct acattcccgt aaatgaataa caaaaggcac
    12301 cggtgcgcta tcttccccta ccgcatgaaa ccggttactg ctgatttgcc ccatattgac
    12361 cgtcatttgt ttatcaccgg cttcaatccg gcaagtttcc gcaataatga cgccctgaaa
    12421 ttgcatattt ccgccgggca gcgtggtgtt ccatttattt ccggccagag caaacattgg
    12481 cagcaacgac gctaatagaa ataatctctt ccttttcatc gttattttta tcgcacaagg
    12541 gtgggcatcc ctgcccgtaa tgacgtccct gaacctgggt aggttattga tactgaacct
    12601 tgaaggtcgc atccgcatta gcagcacccg gggttgcctc gccgattgca taataacgcg
    12661 cctggaacgg aatggtgttg gtaccgttat tcagggttgt ttgtgcacta aatgtcgcac
    12721 cgtccagcgt cagctcattt cctgttctgt ccagtatctg tacaccaaca tttgttgcgc
    12781 tacccgcagc tgaactctgc agagccagta ctttaggatg agcactgtca atcgcagtcc
    12841 ccaagaaggc aacagcggct tttgtggcaa cactggtatc gcaatcattc agctgaatgt
    12901 taaaaccgac agcagagctg gttgctccag tctgcttcag actagcggta cgaacctggc
    12961 ccaactgaac ggtttgatca acagagcctg catcaactgc gcaagcggcg ttaaccactt
    13021 cccctttaaa gtgaacggtc ccaccattta ccgtcgtggg tgtagtttca gccagagccg
    13081 ctgtagaact cagggacaga gccgacagaa caacaattgc cagagtttta attttcatac
    13141 tgctttcctt tcaaaaaact atttctaaat cgacatgggc agtcgttccg tacactttgt
    13201 tttgtcaacg agtttgactg ccaacactgc acagtttccc cccaaaagat gaaacatttg
    13261 gggccatttt gactcataga ggaaagcatc gcgcacaaac tttttcagtt t
//
LOCUS       DAPHBJ010000119        13290 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.120, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000119 DAPHBJ010000000
VERSION     DAPHBJ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.120"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            166..615
                     /gene="rpiB"
                     /locus_tag="RHZ76_003036"
     CDS             166..615
                     /gene="rpiB"
                     /locus_tag="RHZ76_003036"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HDV2813633.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEVDGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
     gene            684..1013
                     /gene="yjdP"
                     /locus_tag="RHZ76_003037"
     CDS             684..1013
                     /gene="yjdP"
                     /locus_tag="RHZ76_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HDV2813634.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(1160..1918)
                     /gene="phnP"
                     /locus_tag="RHZ76_003038"
     CDS             complement(1160..1918)
                     /gene="phnP"
                     /locus_tag="RHZ76_003038"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HDV2813635.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(1920..2354)
                     /gene="phnO"
                     /locus_tag="RHZ76_003039"
     CDS             complement(1920..2354)
                     /gene="phnO"
                     /locus_tag="RHZ76_003039"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HDV2813636.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(2341..2898)
                     /gene="phnN"
                     /locus_tag="RHZ76_003040"
     CDS             complement(2341..2898)
                     /gene="phnN"
                     /locus_tag="RHZ76_003040"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HDV2813637.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(2898..4034)
                     /gene="phnM"
                     /locus_tag="RHZ76_003041"
     CDS             complement(2898..4034)
                     /gene="phnM"
                     /locus_tag="RHZ76_003041"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDV2813638.1"
                     /translation="MIINNVKLVLENEVVSGSLEVQNGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKDNHIHIDHVWRQGKRVF"
     gene            complement(4031..4711)
                     /gene="phnL"
                     /locus_tag="RHZ76_003042"
     CDS             complement(4031..4711)
                     /gene="phnL"
                     /locus_tag="RHZ76_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HDV2813639.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            complement(4822..5580)
                     /gene="phnK"
                     /locus_tag="RHZ76_003043"
     CDS             complement(4822..5580)
                     /gene="phnK"
                     /locus_tag="RHZ76_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HDV2813640.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(5577..6422)
                     /gene="phnJ"
                     /locus_tag="RHZ76_003044"
     CDS             complement(5577..6422)
                     /gene="phnJ"
                     /locus_tag="RHZ76_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDV2813641.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     VIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            complement(6415..7479)
                     /gene="phnI"
                     /locus_tag="RHZ76_003045"
     CDS             complement(6415..7479)
                     /gene="phnI"
                     /locus_tag="RHZ76_003045"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HDV2813642.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTGMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            complement(7479..8063)
                     /gene="phnH"
                     /locus_tag="RHZ76_003046"
     CDS             complement(7479..8063)
                     /gene="phnH"
                     /locus_tag="RHZ76_003046"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDV2813643.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSTPLNNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(8060..8512)
                     /gene="phnG"
                     /locus_tag="RHZ76_003047"
     CDS             complement(8060..8512)
                     /gene="phnG"
                     /locus_tag="RHZ76_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDV2813644.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(8513..9238)
                     /gene="phnF"
                     /locus_tag="RHZ76_003048"
     CDS             complement(8513..9238)
                     /gene="phnF"
                     /locus_tag="RHZ76_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HDV2813645.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(9259..10038)
                     /gene="phnE"
                     /locus_tag="RHZ76_003049"
     CDS             complement(9259..10038)
                     /gene="phnE"
                     /locus_tag="RHZ76_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDV2813646.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(10144..11160)
                     /gene="phnD"
                     /locus_tag="RHZ76_003050"
     CDS             complement(10144..11160)
                     /gene="phnD"
                     /locus_tag="RHZ76_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV2813647.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(11185..11973)
                     /gene="phnC"
                     /locus_tag="RHZ76_003051"
     CDS             complement(11185..11973)
                     /gene="phnC"
                     /locus_tag="RHZ76_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDV2813648.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            complement(12106..12549)
                     /gene="yjdN"
                     /locus_tag="RHZ76_003052"
     CDS             complement(12106..12549)
                     /gene="yjdN"
                     /locus_tag="RHZ76_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HDV2813649.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWHETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(12807..13142)
                     /locus_tag="RHZ76_003053"
     CDS             complement(12807..13142)
                     /locus_tag="RHZ76_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HDV2813650.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT         2633 a         3713 c         4037 g         2907 t
ORIGIN      
        1 gatagatgtt gctggatggt gagtgtcttt aacgctattg cgctgtcaga tgtaactctg
       61 taaaacagat caggaaggcg taattcatcg attttgggtg tttttaattc attaacatca
      121 caaatgtttt ttgattgtga agttttgcac ggacggggaa gatgaatgaa aaagattgca
      181 tttggctgtg atcatgtcgg tttcatttta aaacatgaaa tagtggcaca tttagttgag
      241 cgtggcgttg aagtgattga taaaggaacc tggtcgtcag agcgtactga ttatccacat
      301 tacgccagtc aagtcgcact ggctgttgct ggcggagagg ttgatggcgg gattttgatt
      361 tgtggtactg gcgtcggtat ttcgatagcg gcgaacaagt ttgccggaat tcgcgcggtc
      421 gtctgtagcg aaccttattc cgcgcaactt tcgcggcagc ataacgacac caacgtgctg
      481 gcttttggtt cacgagtggt tggcctcgaa ctggcaaaaa tgattgtgga tgcgtggctg
      541 ggcgcacagt acgaaggcgg tcgtcatcaa caacgcgtgg aggcgattac ggcaatagag
      601 cagcggagaa attgagattc atccactact tgcatggatg agtaatgatt aatgtggata
      661 gagtttcttt ttgaggttgg ctaatgaaac gctttccgct atttctttta ttcaccctgc
      721 tcacgctgtc caccgttccg gcccaggccg atattatcga cgacaccatt ggcaatattc
      781 agcaggcgat taatgacgct tataaccctg accgtgggcg agactatgaa gattcgcgcg
      841 acgacggctg gcagcgtgaa gtgagcgacg atcggcgcag acaatatgac gaccgccgcc
      901 gccagtttga agatcgccgc aggcagttgg acgatcgcca gcgccagctt gatcaggagc
      961 gtcggcaact ggaggatgaa gagcggagaa tggaagatga gtatgggcga tgaggttggg
     1021 tatggagcag gcatcgcccc tcaccccaat ggggagaggg agaaaacgag cgcaatattc
     1081 aatatcgagc acaatcggtc ccctcgcccc tctggggaga gggttagggt gagggggcca
     1141 gcgcgcacat tccctctcat cacgccaccc caatctccat cccatcaaac cccacctcaa
     1201 accctgacgg tagtgcgttt tccatcagcc acgcatcaaa ctggtggctg atatgggtca
     1261 gaatcacccg tggcgagcgg ataacctgat tcagcgcaag cacggtattt aaatcacagt
     1321 gattacgcgg tgcatccgcg cgcggcgggt gactgcaatc catcaccatt acctgcggct
     1381 gattattgcg taaaaatttc agcgtttttt ccggcaagcc tgcggtgtca gacagccacg
     1441 ccacccggct gtgtgccgtt tccagcagat aaccgaaggt cagttttgag tggttgagcg
     1501 gcaggggcgt gacctgtaac ccctgcaaat caaacaccac aaacggttcc accgtgtggc
     1561 tgaaatcaag caggcccgga tgtttaaaca gatcgtcgca gccctgttca tccggcgggc
     1621 cgtaaaccgg gatcggatcg ccaacgcccc agcgcagcgg aaacagcccc tggacgtgat
     1681 ccatatgata atgcgtcagc aaaaactgct ggaacgatcc gggcgaccag cgatcggcga
     1741 gatcgtgcag cccggcgtcg atcagggtga ttgcgtcgtt aaacttcact acgccgctgc
     1801 acggttggcg gcgatactgc ggcgagcgcc gcgctctggc gcaggccgca cactcgcagc
     1861 cccatgccgg aacgccctgt gcgccgccgg tgccggtgag cgtgagggtc aggctcatgt
     1921 tacagcgcct tggtgaagcg gaagtggctc tgctcgtagc cttcgcgcag atagaaacgg
     1981 tgcgcgtcgt ggcgcttcac gttggtcgaa agttcggtca tttcggcccc ggcctggcgg
     2041 gcttcttctt ctgcccacgc cagtaactta ctgccgacgt tcagaccgcg cgcctgcggc
     2101 attaccacca actcctgaat ttcgccgatc cagttgacat gatgcagatg aaactgcaaa
     2161 tgcaggccga tcatgccgac aacttcgcca tcaagcagcg ccagatggta gcgcatgttt
     2221 gggtcgcgca gattggcgtt aaaacccacg cgaaacgcgt ggtggtcaaa ctccgcctgt
     2281 tttagctcac aaatcagcgc gtaaaccgcg tcggtgtcgt actgcgtggc cgggcgaagc
     2341 tcacaagcag gcatggtgtt tctccttctg atggatcagc gtcagcagcg tgtcgaccga
     2401 ctggcgcagg ctgccgtcat tgttgagcgt atggcaatcc tgtggagtat agcgggcggc
     2461 gcgcgccagg cgggcgttaa tttcactggc gttttcacgg ccacggtttt ccaggcgctg
     2521 gcggaggatc tccggcgaaa cctgtaaaca gacgggcagc agcgccgatt gatagcgcgc
     2581 ccgcgcctgc ggcagatggg cgcgtgagcc gttgaccagc acgtcgaatc cggcgtgcag
     2641 ccagagatca atctcgacgc cgacgccata atacagaccg ttagcgtgcc agcttaaggc
     2701 caacagattt tgccccgcgc gggtaaaaaa ctcctgctcg ctcagggcga tatggttttc
     2761 acttccggcg ctggcatcgc gcgtgatgta gcgatgcgcc accagtaact gagtttgttc
     2821 ccgcaggcgg agttccgcca gcaggctgtc tttcccggag ccggacggcc ccattaacca
     2881 aatcagtttt cccatcatca gaacaccctt ttaccctgac gccagacgtg gtcgatatga
     2941 atatgattgt ccttgcgatg cgccagcacc aggtcggcgc gtttgccctc gccaatcacc
     3001 ccgcgatcct ggagattaag cgcctgcgct ggatttttag tcaccagctt caccgcctgc
     3061 ggcagcgtaa agcggttgct ctcgtcatcg gcgacgcgaa atgccgcatc gagcaggctg
     3121 gcggggtagt agtcggaaga gaggatatcc agcaggccaa gctgcgccag ttcactggcc
     3181 gccacgttgc cggagtgcga gccgccgcgc acaatattcg gcgcgcccat cagcacgttc
     3241 atgccatgct tgcgcgaggc ttccgccgct tcgaacgtgg tgggaaattc ggcgatcacg
     3301 ctgccaagct ggtgagattc agcaacgtgg gcgtgggtgg cgtcatcgtg gctggcaagc
     3361 gcaatttttc gcgcgcggca cagggcggcg atggattcgc gattcggctg cgaccagcgt
     3421 gcggcgagcg ccagttgctc ttcttcgtac tgctgcatct gcgcatcagt gagggagtat
     3481 ttgccctgat aatattcgcg atacttctcg cggttggcga actggcgctg gcccggcgag
     3541 tggtccatca gcgacaccag cgtcaccggc tcgcgctgca ccagtttttc aaacagcggc
     3601 agcgtggtgt gatgcggcag ttcgcagcgc agatgcagac ggtgctcggc gcggttgacg
     3661 ccgcgtttct gcgtctcttc gatggcgttg atcatcttct ccagattctc cagccgatcg
     3721 ccgccgtcgc gcacgtcgcc aattgccacg gcatccagta cggtggtgat gccgctcgcc
     3781 accatcagcg cgtcgtggct gctcatcgcc gagtgggcag gccagtcaac tttcgggcgc
     3841 ggggtgaaga atttatccag attatcggta tgcagctcaa tcagccccgg cagcagccag
     3901 ccgccttcgc cgtccatcgc ctccggcagg cggctctggc tttcggcaaa ggcgcggatt
     3961 tcgccgttct gcacctccag cgaaccgctt accacctcgt tttccagcac cagcttaacg
     4021 ttattgataa tcatgaagag gctcccattg ggtgcaggcg gtcggcgacg tcattacgta
     4081 cagcttcgtc atggaagatg cctacgatgg ctgcgccacg ggttttggct tcgcgaatca
     4141 gttccaccac cgcggcgctg tttttggcgt ccagcgaggc ggtaggttcg tcaagcagca
     4201 gaatggggta gtcgacgata aagccgcggg cgatgttgac gcgctgctgt tcgccaccgg
     4261 aaaatgtcga tggtgccagg tgccacaggc gttccggcac gttcaggcgg gtgagaagac
     4321 gcgcggcttt agcggcgcag gcttcacgcg gaacgccggt atcgagcagc ggctgcatca
     4381 ccacttccag tgctgagata cgcgggatga cgcgcagaaa ctggctcacc cagccgacgg
     4441 tggttttgcg gatttccacc actttgcgcg ctggcgcggt gaccaggtct acccactcgt
     4501 caccgtgttt gatctggatt tgaccttcgt cgggtagata gttggcgtac agcgagcgta
     4561 gcagagttga tttgccgctg ccggaatggc cgtggagcac cacgcattcg cccgcgttga
     4621 cggtgagcga ggcgcgattg aggacgggca ggcgcacgcc gttttgctgg tgcaggatga
     4681 aggttttact gacgttttgt acgttaatca tttttcgcct cgtggttcgg gtgtcggatg
     4741 cgacgctgac gcgtcttatc cgacctacgg ggagcgcatt tgtaggccgg ataaggcgtt
     4801 tacgccgcat ccggcaccgg ctcaattctg caaaaccgat gacaccagca gctgtgtata
     4861 cggatgatgc gggtcgtcga gcacgcggtc ggttaaccca ctctccacca cttgcccctg
     4921 cttcatcacc agcaaacggt ccgccagcag gcgggcgacg cctaaatcat gggtgacaat
     4981 caccaccgcg aggttcagct ccaccaccag gccgcgcagc aggtcgagca ggcgggcctg
     5041 caccgacaca tccagcccgc cggtcggttc atccataaac accagcttcg gatgcgtcac
     5101 caggttgcgg gcaatctgca aacgctgctg cataccgccg gaaaaggtgg tcggcaggtc
     5161 gtcgatccgg ttggcgggaa tctccacctc ttccagccac ttctgcgcgg tggcacgaat
     5221 atcgccgtaa tgacgtgccc cggtcgccat cagccgctcg ccgatattgc cgcctgccga
     5281 cacctggcgg cgcaggccgt cgagtggatg ctgatgcacc acgccccatt cggtacgcag
     5341 caggcgacgg cggtcggcct cgctcattgc atacagcgaa cggttctcgt agtgaatttc
     5401 cccctgctgc ggcgtcaggc gcgcggagat cgacttcagc agcgtggtct tcccggagcc
     5461 ggattccccg acaatgccca gcacttcccc cggccataaa tcaaaagaga catcgctaaa
     5521 gcctttgccc ggcgcgtaaa ggtgggtcag gttattgacc gaaagtaacg gttgattcat
     5581 tggttttttg cctcgctctg ttggcggcaa taatcggtat cggagcagac aaacatgcgg
     5641 tttccggcgt catccagcac cacttcatca agatagctgt gggtcgatcc gcagatggcg
     5701 catggctcat cccactgctg aacggtgaac gggtgatcgt cgaaatcgag actttccacg
     5761 cgggtaaacg gcggcaccgc atagatgcgc ttctcgcgcc ccgcgccgaa cagttgcagg
     5821 gcgggcatca tgtccatttt tgggttatcg aatttcggga tcggcgacgg gtccatcacg
     5881 tagcgcccat ttaccttcac cggataggcg taggtggtgg cgatatgacc gaagcgggcg
     5941 atatcttcat acagtttcac ctgcatcacg ccgtactctt ccagcgcgtg catggtgcgg
     6001 gtttccgttt cgcgcggctc gataaagcgc agcggctcgg ggattggcac ctggaagata
     6061 attatctgat cttcggtcag cggcgtttcg gggatgcggt gacgcgtctg gataaccgtc
     6121 gcatcgtccg tacgttccgt ggtgtttacc ccggtcacgc gcttgaagaa gttgcgaatc
     6181 gacacggcgt tggtggtgtc atccgcgccc tggtcaatca ccttcagcac gtcgctttcg
     6241 ccaatcacgc tggcggtaag ctgaatgccg ccggttcccc agccgtacgg catcggcatc
     6301 tcgcgcccgc caaacggcac ctgataaccg gggatcgcca ccgcttttaa gatggcgcgg
     6361 cggatcatgc gtttggtctg ctcgtcgagg taggcaaagt tgtagccgct cagattagcc
     6421 atggttctgc tcctgttgca gacgtttgag tagctccagt tcggcctgga aatcgacgta
     6481 gtgggggagt ttgaggtgtg agacaaagcc tgcggcttcg acgttgtcgg catgcgccag
     6541 cacgaactct tcatcctgcg ccgggcctgt cgcgtgctcg ccgtattccg gggcttgcaa
     6601 cgcacggtca accagcgcca ttgccatcgc tttgcgctcg ctcatgccga ataccagccc
     6661 gtagccgcgc gtgaagtgcg gcggctcatc cggcgggtcg ataaaaccgt tgaccatttc
     6721 acactcggtc atcagcagtt cgccgacgtt taccgcaaat cccagctctt ccggcacaat
     6781 cgatacgtcg atatagccgc tgcggatctc gcccgcgaac gggtggttgc gcccgtaacc
     6841 gcgttgggtg gagtaggcca gcgccagcaa atagccttcg tcgccgcgca tcaactgctg
     6901 caaacgggaa gaacgtgagc aggggtaaac cggcggcgtg cgggtgatgt catccggctg
     6961 tgcgccgcta tcctcttcaa acttcgccag cccctgacgc gccagcaggc tgaaaacgtg
     7021 cggcgacggc tgttgttcgc tgtcggcggt ggtcagcgtc ggcgcttcgc cgtttgccag
     7081 cagggtaaaa tcgagcaggc gatgggtgta gtcgtaggtt gggccaagca gctggccgcc
     7141 gggaatgtct ttataaacgg cggagatacg gcgttcgaga cgcatcccgg tggtgtcgag
     7201 cggctcgctt accgccagct tcgccaacgt ggtgcggtag gcgcgcagca ggaaaatcgc
     7261 ttcaacgtta tcgccgctgg cctgtttcag cgccagcgcc gccagttcgc ggtcggcaat
     7321 gccgccttcg gtcatcacgc gatctaccgc gaggttaagc tgctgttcaa tctgggcgac
     7381 gctcagttca ggcaaatcgg tatcgccccg gcgtcggctc tcttgcaggg cgtgggcggc
     7441 gtcgatcgcc ttttcgcccc cttttacggc aacgtacatc agcacacctc cacatgcgtg
     7501 gttcgcggaa tagccagcag gcgttcgccg caggtcagga tcaggtcgat gccgagcggg
     7561 aacgggtgcg ggcgctcggt gagttcgtgc agaatgcact ccggcagctg cggagcgatc
     7621 attcgttctt cggcaatacc cgcgccggtg agacgcaaca tgcgcccgcc gctcaggctg
     7681 gcgacctgta aaatcagcgt cgcgcccgct tccggcgcaa cggcggtgcc ggtggaaagg
     7741 gcgttgagct gttcgctgga aatcgcctca tccgtcaccg cgaaggtcgc ctgttccggc
     7801 tggctgacca gcggcgcgtt ggtatgaaaa cgcaggctct ggttgacgat atcgttattt
     7861 aatggggtag aaagccacac cggcgtgtcg ttatcggcca gcgtcagcag cacgctggtg
     7921 gtggcgatat tcagcggttg ccagccgcgt ttgagctgat gcagggcgac aatcacgccc
     7981 ggctcgctca tggcctttaa caggcgacga aaactgtgct gggcatcctg cacgggaagc
     8041 ataaaagcgg tttccagggt catgcgttgt ctccgcgaac catcgtaaag aagtcgaccc
     8101 ggctggcgtt cacttcggcc tggcgtgcgg caatgcgtgc catacggtca gcgtccagcg
     8161 gggcaataag ggtttctgat aagttttgaa agtggcgaga ttgctgcatc agcgcgtcaa
     8221 tcagcgcgca gcgttcggcg tgctgtttat cacgccccag cacccagctg tagccgagcg
     8281 tgccgtcagt caggcgcacg gcggcgcggg tcagcgtggc gtcgccggca aaaaaacgtt
     8341 cgccggtgcc gcccatccgc gcctgaatct gtaccaggcc agtttcagcg gcgcggatca
     8401 cctcatagtc ggcggtgatg tttagcgcgt tcaggcgtgc tgccagttca gccggttggc
     8461 tgtgcgccag cacggacatc cagtgctggc gggtcgcggt atctgcgtgc attcagtgct
     8521 ccatagtgaa ttcaatcatg tcggcgcgcg tcaggctgac ggagtactcc gccgggctgc
     8581 tttcaccgtc acggtggtta agggtgcgca cgcacagcag cggcgacata ttcgggattt
     8641 caagacgctg gcactctttg gcctgggcgc ggcgggcgct gatccgcgtc tggctgcggc
     8701 gcagcgcaat tccggtttgc tcgcgcagaa aatcgtgcag cgagccgctg tcgaagcgtt
     8761 gcagcgtcgg ccagagggtg aggtccgcga agtagtggtc gattaaacag agcgcgacgc
     8821 cgttgacccg acgcagggtg cgcaggtgga tgacgttctc cccctcggta atccccagtg
     8881 cgtcagcgac gtggccggac gcggggcgca ataccgaaag cagtttttcg ctggtgggat
     8941 ggctgccctg atccagcaga ttctggctaa aacgcgcctg ggcgttgagc gggtaatcga
     9001 acgggcgcat cagcaccagc acgccgacgc cctgacggcg ctgtacccag cctttttcca
     9061 ccagttggtc gatggcgcgg cgcagggtgt ggcgattcac ctcaaagcgc gctgccagtt
     9121 gctgctcggc gggaagatag tcgccgcagc ggtagtgttg acgaagctcc tgctcaagtt
     9181 ttgcggctat ctcttgatag cgtgttgggt agctggtcgg atgtgtagac aagtgcatag
     9241 atatcaatgc ctcgcttatc agataaagtg cttacgcaac cgttgagaga ggaaatccag
     9301 caggctgacc gtgacgatga taagcaccat cagggcgcag gtttgttgga actggaaacc
     9361 gcgaatcgct tcccacaggg tgacgccgat cccgcccgcg ccgaccatgc cgacgacggt
     9421 cgccgagcgg acgttggatt cgaagcgata gagggagtag gagatcagca gcggcatcac
     9481 ctgcggcagc acgccgtaga ggatctcttc gagcttgttg gcaccggtgg cgcgaatgcc
     9541 ttccaccggg ccaggttcaa ttgcttctac cgcttcggaa agcagcttgg agagcacgcc
     9601 ggtggtgtgg ataaatagcg ccagcacgcc agcgaacggt ccgagaccga cggcgaccac
     9661 gaacagcatg gcgaagacca tttcgttaat ggcgcggcag gcgtccatca ggcggcgaac
     9721 gggctggtaa acccaccacg gcaccaggtt ttcggcgctc atcaggccaa aggggatgga
     9781 gagaaccacc gccagcgcgg tgccccagac ggcgatttgc agcgtgacgg ccatttcggt
     9841 gaggtaatcc tgccactggc tgaaatcggg cgggaagaag tcggcagcga aggttgccat
     9901 gttgccgccg tctttaatca gcgtgagcgg ggccatttcc gcgccctgcc acgagacgac
     9961 cagcacggcg agaacaacgg cccagctcag aagcgagaac cagctgcgct tgggtggggc
    10021 gatggtgatg gtttgcatgt ttggctccgg tctgtaggcc ggataaggtg cttgcaccgc
    10081 atccggcatc aacgcctgca cattgtcgga tgcgacgccg gggcgtctta tccgacctac
    10141 gatttactgc accgctttac tcaccgaact catcgcgctt agcgcgttgt tcaggcggtc
    10201 caggtcatcc agctgcgcct gaatctcggt ggtttttgcc agcttgtcct gctcattcag
    10261 tcctttattg cttttcacgc cctgcatctc tttaaacagc gcgagctggc gaatcggcac
    10321 cagttgcagg tcgctggaag cgcggaatgg cgcccagccc aggcgttcca gcaccgcttt
    10381 ttcttccggc gttttgccgt agttcataaa gaagtcgtag atcttgtctt tggtggtttc
    10441 ggaaagattc ttgcgccaga cgatcggatc gcctgggatc agcggcgact tccagatcac
    10501 cttcagttct ttcagcttct ctggcgcgga ggttttcagc ttgtcgaggt tttcggtgtt
    10561 gttggtggca acatccacct gcttgttggc gacggccagc gcgttggttt catgcccggc
    10621 gttgacggtg cgcttgaagt cgctggcgga gatattgttt ttggcaaaga cgtagtaacc
    10681 ggggacgagg aagccagagg tggagttagg atcgccattg ccgaaggtga gatctttccg
    10741 cttcgccagc agatcgttca ggttgttgat cggactatct ttgttgacga tcaacacgct
    10801 ccagtaaccc ggcgatccgt ccgccgcgac ggtctgagcg aagacctggc cgttggcgcg
    10861 atccaccgct tccattgccg acaggttgcc gtaccaggcg atatccactt tattgaagcg
    10921 cattccctgg ataatgcctg cgtagtctgg ggcaaagaag gcgttcacct tcacgcccag
    10981 cttcttctcc atatcctgta agaatggcgt ccattgcggt ttcaggtttt gctgtgattc
    11041 cgttgaaata atgccgaaat tcagcgcctt ttcctgctct tcggcgtgtg ccgggcttaa
    11101 cagggtgctg aggctgaaca tgctggtgaa ggccagcgag gcaattatct tagcgttcat
    11161 tcgttttcct caatgatggg gatgtcaggc agctttcgcg ttctcttcga tgcgattaat
    11221 gctgcggtag agatggtcaa aacgttcgtt atcaaactgt tggctgctgc cgtcgtagaa
    11281 aacgtgcccc tggcgcaggg cgacgatgcg ttcgcagtag cgcagggcgt aatccacctg
    11341 atgcagcgtg acgaccacgg tgatgccgtc gttctgattg atgtcgcgca gggtgtccat
    11401 cacgatgcgg gcggattccg ggtccagcga ggcgatgggt tcatcggcca gaatcacctt
    11461 cgcctgctgc atcagcgcgc gggcaatcgc cacacgctgc tgctgtccgc cggagagggt
    11521 ggaaacgcgt tgatgggcaa aatgcaccat gccaacgcgg gtcagcgcct gtaacgcgcg
    11581 ttgtttctgc tcgcgggtaa accagctaaa acaggtgcgc cagaacggcg tgctgccgag
    11641 cgcgccaatc agcacgttct ccagtacgct caggcggttc accaggttga attgttggaa
    11701 gatgtagccg gtgttggcgc ggcttttgcg gatatcgcgc gccagacggc cttcgcgctg
    11761 gactgtgcgg cccagcagct cgatatggct gccggcggat ttatcgccgg taatcaaacc
    11821 gcttaagtga cgtaaaaggg tggatttgcc ggaacccgac ggcccaagca gagccaccat
    11881 ttcaccgtga tgaatgttca gatcaaccgc atgcagcgcc tgatgctgat tgaaggtttt
    11941 ggcgagcttc tcgacacgga taatcgtttg catgatgcag cctccctaaa aaagtggctc
    12001 catcgtggcg gattattgtg acgatttggt taattaaaag tgactaacag atgaagagtt
    12061 aacgggaatt cgatgacagt gcgggagggc gggcctcccg gcgggttatt gcgttggttg
    12121 ttgtttgacg acattaatca tccacggtac gccaaattta tcggtgactt tgccaaagcc
    12181 atgcgcccag aaagtttcgt gccaggccat ttcgattttt ccgttagcgg caagattgtc
    12241 aaaccagcgt tttccttctt cgacctgttg cgaatcgagc accagcgtaa agccggagta
    12301 gctggctttt cctgacggca tggcatcgct catcatgatg tcgcttccgg caatgcgcac
    12361 gttggcatga gcgatggcgg tatcgggaaa ttgcattccg gaagggcagt tctcggcgct
    12421 gtcctgcgct gattttggca tttcgccgaa gctgatttta tagagcagtt ccgcgcccaa
    12481 cgtacgttga taataggcaa tcgcgtcgga acagttaccg gcaaaagaga ggtagggact
    12541 taacggcatg atggtgacct cagttaagag aagccagtta agtgtagttc gaaatttata
    12601 cagatgagag gcggcctgat aagacgcgcg agcgtcgcat caggcagtcg gcactgttgc
    12661 cggatgcggc gtaaacgcct tatccggcct acggagggtg cgggaatttg taggcctgat
    12721 aagacgcgca agcgtcgcat caggcatctg gcaccgttgc cggatgcggc gcgagcgcct
    12781 tatccggctt accgatcaca atacaatcag ttctttttca caaactcaga tttcagtttc
    12841 atcggaccaa aaccgtcgat tttgcaatcg atgttatggt cgccttcaac caggcggatg
    12901 tttttcactt tggtgccaat tttcagcatc gaagagctac ctttcacctt cagatcttta
    12961 atgatggtaa cgctgtcgcc gtcagccagc agattgccgt tagcatcttt aacgatcagc
    13021 tcgtcgcttt cctgtgcagg ttctgcgtcg ttccattcgt aggcacattc cgggcagatg
    13081 tacatgccgt tatcttcgta ggtgtattcg gagttgcatt ttgggcagtg tggtaatgac
    13141 atgtggattt cctcaaaagt cagcagggca aaagcgccaa aaaacggcag attgccgaaa
    13201 aaggccgcaa ttatacacaa aatccctgag tttgtcgggg gtactgctga ttttagacga
    13261 cgatcgtttt cacttgaaag gaaacgcaaa
//
LOCUS       DAPHBJ010000120        13114 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.121, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000120 DAPHBJ010000000
VERSION     DAPHBJ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.121"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..403)
                     /locus_tag="RHZ76_003054"
     CDS             complement(<1..403)
                     /locus_tag="RHZ76_003054"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813651.1"
                     /translation="MKVVKGLVRELSWLKKEQRACHFFLFHLITDFSARAKQGIALQV
                     KDSSDLSEQQDWAQSLMSEFDGPLQQAEAGELPPPPELPEVLIPQPGHYLGIETAHEK
                     TVSLENSSIVNINFIDGKKVVDSKNYNNLPPV"
     gene            904..1110
                     /locus_tag="RHZ76_003055"
     CDS             904..1110
                     /locus_tag="RHZ76_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDV2813652.1"
                     /translation="MTTRLSLTEDQFVDMAFITSLLQMTDKWIYKLIKDGVFPKPIKL
                     GRSSRWLKSEVENWLQERIDQSRK"
     gene            1207..1800
                     /locus_tag="RHZ76_003056"
     CDS             1207..1800
                     /locus_tag="RHZ76_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813653.1"
                     /translation="MKDYKPRYEELYKYYQVWLTDFTKLTVRSGMCHQNIYHHHTLTV
                     GSLKFPGEEEVIAVVPQCLHRIIYGRAAAPLTVNDDLSVSLFTQEHLLAHHPMLEGIL
                     LSECVRLKQRPLANKLISLFRQFSGSELRLKLVWLCWYDLMLGNCLDDWTDNLRFKKD
                     KEMDIWINDRQAENPALTSLMDEYVCFAWRTTAEPLN"
     gene            2392..2961
                     /locus_tag="RHZ76_003057"
     CDS             2392..2961
                     /locus_tag="RHZ76_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HDV2813654.1"
                     /translation="MKPYNANPNYVMNGPLLEDINAHMEAMFQRFAKLLPFRIDFAYR
                     KNTASFGHACKHTMCTEIRHLLAETENALAGFYWVMEYTPKKGLHIHLLGYLNGQYHQ
                     NPYLLSRTMGNIWKSATEGDGYHYLCRTKENYPVRVDQVIHYTDTPAIKALRYAISYL
                     AKVKQKENGLILGRSTVPDKSSRGRPRQG"
     gene            3285..3683
                     /locus_tag="RHZ76_003058"
     CDS             3285..3683
                     /locus_tag="RHZ76_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813655.1"
                     /translation="MYAKSFIALDGNGRLIGARTAQTAPYDRYTCHLCGSTLQYHPEY
                     DTERPWFEHISHNLTENGQQHCPYAKPDKNEIRLVHRLRIFVPNVTPIVLRDSWHCSL
                     CDSDYHGERYCLSCQTGRFSLKLRAESCYV"
     gene            3858..4181
                     /locus_tag="RHZ76_003059"
     CDS             3858..4181
                     /locus_tag="RHZ76_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2813656.1"
                     /translation="MELSKAAETLKELGHPTRLSLYRELVKAGHEGLPVGELQKRLEI
                     PASTLSHHLSSLISVSLIRQERQGRTLFCHARYENLEALIAFLTEECCSGTDDCLTMP
                     QKTEK"
     gene            4183..4896
                     /gene="arsH"
                     /locus_tag="RHZ76_003060"
     CDS             4183..4896
                     /gene="arsH"
                     /locus_tag="RHZ76_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical resistance protein ArsH"
                     /protein_id="HDV2813657.1"
                     /translation="MTGDLKNVDSGLFDTSITEARLGVPAVPHPPRILMLYGSVRERS
                     YSRLATEEAARLLTAMGADVRIFNPSGLPLPDDAPDSHPKVMELRELVRWSEGMVWCS
                     PERHGAMTGIMKAQIDWIPLSEGAVRPSQGKTLAVMQVCGGSQSFNAVNQMRILGRWM
                     RMITIPNQSSVAKAWQEFDEDGRMKPSSYYDRIVDVMEELVKFTLLTRGNLAYLVNRY
                     SERKESAEELSRRVNQSKI"
     gene            4905..5450
                     /locus_tag="RHZ76_003061"
     CDS             4905..5450
                     /locus_tag="RHZ76_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDV2813658.1"
                     /translation="MSDVTAFNNCMSVFWRQHEAELSRFLTSKTGDSEKAADLLQELF
                     LRARAHSDSFCEMENPRAWLYRAARNLLIDEYRTTREFVELEEDAPLTDASPDAISTL
                     DICLPETLQALSEDERWLIEESDLNRRPQQSLADELGISLAAFKSRLLRARKHLKETM
                     TELCQIEADDTSPVCCHKKMN"
     gene            5526..5888
                     /locus_tag="RHZ76_003062"
     CDS             5526..5888
                     /locus_tag="RHZ76_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HDV2813659.1"
                     /translation="MSKIEIFEAAGCCATSSVVVSDEAVKWNASAEWAKKNGVDIQRY
                     SLAKNPQQFLNNPVIKEFLNTSGLESLPVTLLDGKLVMAGKLPNREDIARWAGIQLPQ
                     DWSEDSTQPSCCCIPRMP"
     gene            5915..7666
                     /gene="arsA"
                     /locus_tag="RHZ76_003063"
     CDS             5915..7666
                     /gene="arsA"
                     /locus_tag="RHZ76_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical pump-driving ATPase"
                     /protein_id="HDV2813660.1"
                     /translation="MPFLKDIPPFIFFTGKGGVGKTSLACATAVWLADQGKRTLLVST
                     DPASNVGQVFSQTIGHRITDISTVQNLAAMEVDPMAAAQAYRDRVLDPVRGLMSAEVV
                     SSIEEQLSGSCTTEIAAFDEFTGLLTNHELREKYDHIVFDTAPTGHTIRMLELPGAWS
                     GYLEANPDAAANLGPLVGLEKQQHQYSDAVKALSDAALTRLVLVARAQASTLKEVSHT
                     HDELSAIGLQHQHLAINGVLPPFVGENDPLAQSILAREGKALLAMPENLANLPRSQLF
                     LKPFNLVGLEALRELFTESKASPVLAATTLNALDLPKLASLVDELSQTGKGLIMTMGK
                     GGVGKTTVAASVAVSLAKRGHKVHLTTSDPAAHLSYTLDGSLPNLQVSRIDPKVETER
                     YRRFVLENQGKGLDAEGLAVLEEDLRSPCTEEIAVFQAFSRIIKEADDHFVIMDTAPT
                     GHTLLLLDATGAYHREMVRQMGQTHDHVLTPMMQLQDPEKTKVIIVTLAETTPVLEAA
                     NLQQDLRRAGIEPWAWVINSSLAAAKPSSPFLVTRASRELPLINDVTEQFAKRIALTP
                     LQNEEPVGAALLAKMAD"
     gene            7791..8327
                     /locus_tag="RHZ76_003064"
     CDS             7791..8327
                     /locus_tag="RHZ76_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV2813661.1"
                     /translation="MHIIKAEEQHITAIRNIYAHHVLHGTGSFETEPPDTQEMLNRLR
                     SIQSRGFPWYVALQGDTIIGYCYISRYRERHAYRFTVENSIYIDPVYQRQGVGKALLD
                     HALKWAESQGYRQMIAVVGDSANVASVALHIRAGFTEIGTLKDIGFKHGRWLDTVLLQ
                     MKLGEGNSTLPEDSDLML"
     gene            complement(8362..8787)
                     /gene="arsC"
                     /locus_tag="RHZ76_003065"
     CDS             complement(8362..8787)
                     /gene="arsC"
                     /locus_tag="RHZ76_003065"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HDV2813662.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGNEPTVIHYLENPPSRDEL
                     VKLIADMGITVRALLRKNVEPYEELGLAEDKFTDDQLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLDILPDAQKGAFAKEDGEKVVDETGKRLK"
     gene            complement(8800..10089)
                     /gene="arsB"
                     /locus_tag="RHZ76_003066"
     CDS             complement(8800..10089)
                     /gene="arsB"
                     /locus_tag="RHZ76_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HDV2813663.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSAMLGAGLALISGVVHVG
                     DIPVVWNIVWNATATFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTYIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFTEYASVMVPVDIAAIIATLVMLHLFFRKDIPPTYDLALLKAPAKAI
                     KDLATFRTGWIVLILLLVGFFVLEPLGIPVSAIAAVGAVILFAVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADKGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTATGVIKEAMIYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTITWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(10137..11888)
                     /gene="arsA"
                     /locus_tag="RHZ76_003067"
     CDS             complement(10137..11888)
                     /gene="arsA"
                     /locus_tag="RHZ76_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter ATPase subunit ArsA"
                     /protein_id="HDV2813664.1"
                     /translation="MKFLQNIPPYLFFTGKGGVGKTSISCATAIRLAEQGKRVLLVST
                     DPASNVGQVFDQAIGNTIRPVAAVPGLSALEIDPQEAAKQYRSRIVDPIKGLLPDDVV
                     NSISEQLSGACTTEIAAFDEFTGLLTDASLLTRFDHIIFDTAPTGHTIRLLQLPGAWS
                     SFIESNPDGASCLGPMAGLEKQREQYAHAVEALSDPDRTRLVLVARLQKSTLQEVART
                     HEELAAIGLKNQYLVINGVLPTTEAEHDALAAAIWQREQEALANLPSGLSELPTDTLL
                     LQPVNMVGVSALKGLLDTCSERLPLPATNVLYTPENLSLSGLVDDIARSEHGLIMLMG
                     KGGVGKTTMAAAIAVRLADMGFDVHLTTSDPAAHLSATLNGSLKNLQVSRINPHDETE
                     RYRQHVLETKGRDLDEAGKRLLEEDLRSPCTEEIAVFQAFSRVIREAGKRFVVMDTAP
                     TGHTLLLLDATGAYHREIAKKMGDKGHFSTPMMQLQDPERTKVLLVTLPETTPVLEAA
                     NLQADLERAGIHPWGWIINNSLSIADTRSPLLCQRAHQEQPQIEAVKNQHADRIALVP
                     VLASEPAGIEKLRELMS"
     gene            complement(11906..12268)
                     /gene="arsD"
                     /locus_tag="RHZ76_003068"
     CDS             complement(11906..12268)
                     /gene="arsD"
                     /locus_tag="RHZ76_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter metallochaperone
                     ArsD"
                     /protein_id="HDV2813665.1"
                     /translation="MKTLTVFDPAMCCSTGVCGSDVDQVLVDFSADVQWLKGRGVQVE
                     RYNLAQQPMSFVQNEKAKAFLEASGAEGLPLLLLDGETVMAGRYPKRAELARWFGIPL
                     EKVGLAPTSCCGGNTSCC"
     gene            complement(12316..12666)
                     /gene="arsR"
                     /locus_tag="RHZ76_003069"
     CDS             complement(12316..12666)
                     /gene="arsR"
                     /locus_tag="RHZ76_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HDV2813666.1"
                     /translation="MIFTALQLFKNLSDETRLGIVLLLREMGELCVCDLCTALEQSQP
                     KISRHLAMLRESGLLLDRKQGKWVHYRLSPHIPSWAAQVIEQAWLSQQDDVQAIARKL
                     ASANCSGSGKAVCI"
     gene            complement(12798..>13114)
                     /locus_tag="RHZ76_003070"
     CDS             complement(12798..>13114)
                     /locus_tag="RHZ76_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813667.1"
                     /translation="LDAILKEDIDDRNFDLILSFVGKNLPTDTQSFISMSEYAEDNLL
                     KDGKEISAFRRAKVKDIKNRYFPDGTNLFFIGDGKHERTFLSKPLDLNLDIFVDTCRL
                     TE"
BASE COUNT         3439 a         3319 c         3191 g         3165 t
ORIGIN      
        1 ttactggcgg aaggttgtta taattcttac tgtcaaccac ttttttacca tcgatgaaat
       61 taatatttac tatcgaagaa ttctccaggg acacggtttt ttcatgtgca gtttcaattc
      121 caagataatg acctggttgt ggaattaata cctctggtaa ttctggtgga ggtggaagct
      181 ccccagcttc tgcctgctgt aaagggccat caaactctga catcaatgat tgagcccagt
      241 cttgttgttc gctcaaatct gaactgtcct taacctgcag agcaatgccc tgttttgccc
      301 gtgctgaaaa atccgtaatc aaatggaaca aaaaaaagtg acacgctctc tgttcttttt
      361 ttaaccaact tagttctctt accaacccct tcactacttt cattcttcat ttctcaaata
      421 attatcattt gcatttgtat tgatttaaga aaattttaac atcaacaata tagtgaataa
      481 agtacagatg atcacatagt gaaagcaaac attaaacaaa gaaaatatga ttcaaaagca
      541 taataaattc gtagggtatt ttattggtta aacagcataa aaatgggctt tgtagctatt
      601 tttttacgga tttttccatc gtgttaccag ctttggttgc tttgcttttt catgtctggc
      661 tttacggaaa aagatcgtgg taagtggctt gttcctgcgc aggtatttgt ttgagcgggc
      721 ccgggcatgc ggtcacaaaa acggttatga aatgaggtgg ccacttcctg cagttgacgg
      781 gaggcgacag gaggtcaatg tacctcgctt tttttacgta tcagcccggt aggtatgtcg
      841 gagcagtggt cgttatcaaa catcactcat ccggcttacc tttcttaacc ggagatacaa
      901 atcatgacta cccgcctttc acttaccgaa gaccagttcg ttgacatggc atttatcacc
      961 agcctactgc aaatgacaga caagtggatt tacaagctca ttaaggacgg cgtctttccc
     1021 aaacccatca agctggggcg aagctcccga tggttaaaaa gcgaagtcga aaactggcta
     1081 caggaaagaa tcgaccagtc ccgtaaataa cctcccgcca gactaaaccc tcagcagaat
     1141 aatgactatc cggttgctcc ggaggttttc tgctgtctgt catttaagcc agaaggaata
     1201 tgaaccatga aagattacaa accgcgttac gaagaacttt ataaatatta tcaggtctgg
     1261 ttgaccgatt ttacgaaact gacggtcaga tcgggtatgt gccatcagaa tatctaccac
     1321 caccataccc tgacggttgg cagccttaag tttccgggag aggaagaggt tattgctgtt
     1381 gttccccagt gtctgcaccg gattatttat ggcagagcag cagccccact gactgttaat
     1441 gatgacctga gtgtcagttt atttacccag gaacacttgc tggcccacca tcccatgctg
     1501 gaggggattt tgctgtctga gtgcgtgcgt ttaaaacaac gccctctggc aaataagctc
     1561 atcagtttgt tccgccagtt cagcggtagc gagctgcgtc tgaagctggt ctggctgtgc
     1621 tggtacgatt tgatgctagg gaattgtctt gatgactgga ccgataatct caggttcaaa
     1681 aaagacaaag agatggatat ctggatcaat gaccggcagg ctgaaaaccc tgcgctgacc
     1741 agtctgatgg acgagtatgt ctgctttgca tggcggacga ctgccgaacc acttaattag
     1801 actccgatgg ctgagagtaa atacagcttc tgcagggtaa aacaatggcc atttctgaag
     1861 agactacctt tggagtggtt tttgttcaga aaatattctg cgaagctgca tattcagtcg
     1921 ttggtgatac tggcttaaac atcaactgat gcgatcagaa atgaataatt aaggttaatt
     1981 aatattattt atagaagtca gtaagttatg cctgtctcac tgtaagtctc catgtgtagc
     2041 tatgtcgtgc aaccccatgc ccggtagtcg taatccagtc agtcaccacc gtgtctggct
     2101 agtaacaatc cgctcatatc aatagccctc tgcgaagtca gtcctgcact ggcgaatgtt
     2161 gttggtaaca gttgccagtg cagcttattg cctggaaggg tattcgcatg agatcgaaca
     2221 tcagtttgtg aaaacaagat taacaacagg cgggtatacg tcacatggca ttgttaccgc
     2281 aattgcggtc tgctgttgcc acatcattct acccgataca cccgattcca tggggaggtg
     2341 ttcataaatt acgccacacc catttccttt gactcaacac gaggaattcc aatgaagcct
     2401 tacaacgcca atcccaacta cgtcatgaac ggtcctctcc tggaggacat aaacgcccac
     2461 atggaagcga tgtttcagcg ttttgctaag ctgttaccct tcaggattga ttttgcttac
     2521 cggaaaaaca ctgccagttt cgggcatgcc tgtaaacaca ctatgtgtac agaaatccgt
     2581 cacctgcttg ccgaaacaga aaacgccctg gcaggttttt actgggtgat ggaatacacg
     2641 ccgaagaaag gtctgcatat ccatttactc ggttatctga acgggcaata tcaccagaac
     2701 ccttacctgc tctcccgaac gatggggaat atctggaaaa gtgcaaccga aggagacgga
     2761 taccactacc tgtgccggac taaagagaat tacccggtcc gagtcgacca ggttatccac
     2821 tataccgata cccctgcaat taaagccctg cggtatgcca tcagctatct cgcgaaagtt
     2881 aaacagaaag agaatggcct gattctgggg cgaagtacgg taccggataa aagcagcagg
     2941 ggcaggccgc ggcagggctg aatgtctgca accgttacga tgttaaattt gtacctgcat
     3001 cagcatgccc gacatgacca caacccggta aaagcatggt cttctgctct gcggctgcaa
     3061 tgaagtacca ccgcgccaac tgacgggacg accacgatgc aggagatgat cattttttac
     3121 gtcagtgcac cataatgtga caactaacag acagtcacga ctgctaacgc agacttactc
     3181 cccttctgat acttcttaac tggcccgcca ttcacaccct gaccacactg cccggaccgg
     3241 gacggctgac gcctgcgtgt tgtctggcgg taaggaacaa tactatgtac gcaaaatcct
     3301 ttatcgctct cgatggtaat ggtcgtctga ttggtgcacg taccgcgcag acagcccctt
     3361 atgatcgcta tacatgccac ctctgcggca gcacgctgca ataccatcca gaatacgaca
     3421 cggagcgccc atggtttgaa catatcagcc acaacttgac ggaaaatgga cagcaacatt
     3481 gcccttacgc aaagcctgac aagaatgaga ttagattggt acatcgtttg cgaatatttg
     3541 tgcctaacgt cacccctata gtgttaagag acagctggca ttgttcgcta tgtgattcag
     3601 attatcatgg ggaacgttac tgccttagct gccaaacagg gcgttttagc ctcaagctga
     3661 gagcagaatc atgctatgtg tgatgcccta ccagcagtgg aacagccctt caactctggc
     3721 taaacatagt aaccactgga acctccgttg cagattaagt cgtttctcca gctcgactct
     3781 caggcatgtt gactatggtt tcctgggaaa ataagttatt atttccataa ttccggaaat
     3841 atagaaatga gatgcaaatg gagctttcga aagcagcaga aaccctgaaa gaactgggtc
     3901 atcctacacg cctgagcttg tatagagagc tggttaaagc cgggcatgaa ggtctaccgg
     3961 ttggcgagct gcagaagcgg ctcgaaatcc cagcgtctac gctgagccat catctttcct
     4021 cgttgatatc agtttctctg atacggcagg aacgtcaggg tagaacgctc ttttgccatg
     4081 ctcgatatga aaaccttgag gctctgattg ccttcctgac agaagaatgc tgctcaggga
     4141 ctgacgactg cctcaccatg ccccagaaga cggagaagta aaatgacagg tgatcttaag
     4201 aatgttgatt cgggtttgtt cgacacttca atcacagagg caagattagg tgtccctgcc
     4261 gttcctcatc ctccgcgaat cctcatgttg tacggctctg tgcgcgaacg ttcctacagc
     4321 cgactggcaa cggaagaagc ggcaaggtta ctgaccgcaa tgggcgcaga cgtgcggatc
     4381 ttcaatcctt ctggcttgcc tcttcctgat gatgcacctg actcgcatcc taaagtcatg
     4441 gaactgcgcg agctggttcg ttggtccgaa gggatggtgt ggtgttcccc tgaacgccat
     4501 ggtgcgatga ccggcatcat gaaggcccag attgattgga taccgttatc agaaggtgca
     4561 gttcgccctt cgcaaggtaa gacgctggcc gtaatgcagg tctgcggtgg ctctcagtct
     4621 ttcaatgccg taaaccagat gcgcattctt ggccgctgga tgcggatgat caccatcccg
     4681 aatcagtcct ctgtcgcaaa agcctggcag gagtttgatg aagatggacg catgaagccg
     4741 tcgtcttatt acgaccggat cgtggatgtg atggaagaac tggtgaaatt tactctgctg
     4801 acccgaggta acttagccta tctcgttaat cgctacagcg aacgaaaaga gtctgcagaa
     4861 gaactttccc ggcgggttaa tcagagcaaa atctgagggc tggtatgagt gatgtaacgg
     4921 cattcaacaa ctgcatgagc gtgttctggc ggcaacatga agccgaactc tctcgcttcc
     4981 tgacatcgaa gacaggtgat agcgaaaaag cagccgacct tctgcaagag ctattcctgc
     5041 gtgcccgcgc tcattcagac agtttctgcg agatggagaa tccgcgagcc tggctgtatc
     5101 gggctgcgag gaatctacta attgatgagt accgcaccac cagggaattc gttgagctgg
     5161 aagaagatgc cccattaact gatgcatccc ctgatgcaat ctcaacgctg gatatttgcc
     5221 tgcctgaaac attacaggct ttatccgaag atgagcggtg gttgatcgaa gagtccgacc
     5281 ttaaccggcg tccccagcag tctctggccg atgaacttgg aatctcgctt gcggcgttta
     5341 aatcccgttt gctacgggcc agaaagcacc tgaaagaaac gatgacagaa ctttgtcaga
     5401 ttgaggcgga tgacacgtct cccgtctgct gtcataaaaa aatgaattaa tcgcgcatct
     5461 ttttcccacc accttcgttt acctcagtgt aaagccaaat gactaaacac tgaggtaaac
     5521 aatccatgtc aaaaattgag atctttgaag ctgcaggctg ctgcgcaacc agtagcgttg
     5581 tggtcagtga cgaagccgtc aaatggaacg ccagcgccga atgggcaaaa aaaaatggcg
     5641 tcgatattca gcgctacagc cttgcgaaaa acccgcagca gttcctgaat aaccctgtca
     5701 tcaaagagtt tctgaacact tcgggactag agtcccttcc agtgaccttg ctcgacggca
     5761 agctcgtaat ggcaggcaaa ctcccgaatc gtgaagatat cgcccgttgg gccggtattc
     5821 agcttcctca ggactggagt gaagacagca cgcaaccaag ctgctgctgc attccacgta
     5881 tgccttaatt acagaggagc tatcactgat gaatatgcca tttttaaaag acattccccc
     5941 gttcatcttc ttcaccggta aaggtggagt aggtaaaaca tctctggcct gtgctactgc
     6001 ggtatggctg gccgatcagg gcaagagaac acttctggtg agtaccgatc cggcttccaa
     6061 tgtcggtcaa gtatttagcc agactatcgg ccaccgaata acagatatca gtaccgtcca
     6121 aaatcttgcg gcgatggaag ttgacccgat ggctgctgca caagcctatc gtgaccgagt
     6181 gctcgacccg gttcgcgggc tgatgtcagc cgaggtggta agcagcattg aagaacagct
     6241 ttcaggttca tgcaccacgg aaatcgcggc atttgatgag tttacaggtc ttcttaccaa
     6301 ccatgagctg cgggaaaagt atgaccatat cgtatttgat actgcgccaa ccggtcatac
     6361 aatccgtatg cttgagttgc cgggggcctg gagcggttat ctcgaagcga accctgatgc
     6421 cgccgctaac ctcggaccgc tggtggggct ggagaaacaa cagcaccagt actcagacgc
     6481 ggttaaggct ctctctgatg ctgcactcac gcgtctggtt ctggttgccc gcgcccaggc
     6541 ttcgacactg aaagaagtct ctcatacgca cgatgagctg tctgctatcg gtttgcagca
     6601 tcaacacctt gccattaatg gtgtcttgcc accatttgtg ggtgaaaatg atccgctggc
     6661 gcaaagtatt ttggctcggg agggaaaagc actacttgct atgccagaga atctggcgaa
     6721 ccttccccgc tcgcagctgt ttctgaagcc atttaatctg gtcggtctgg aagccctgcg
     6781 ggagctattt acggagagca aagcctcacc ggttctcgct gctaccacgc tgaatgctct
     6841 ggatttaccg aaacttgcct cgctggtaga tgaactaagc cagacaggta aagggctgat
     6901 aatgacgatg ggtaaaggcg gagtgggtaa aacgaccgta gcggcctcgg tagcggtatc
     6961 gctggctaag cgcggtcaca aggtccactt gaccacatcc gacccggcag ctcatctgtc
     7021 ttatacgctg gatggctcgc taccaaatct tcaggtcagc cgtatcgacc cgaaggtaga
     7081 gacggaacgc tatcgtcgct ttgttctgga aaatcagggg aaagggttag atgcagaagg
     7141 gctggcggtg ctggaggaag atctccgctc accctgtact gaagagatcg ccgtcttcca
     7201 ggcattctcg cggatcatca aagaggccga tgaccatttt gtcattatgg acacagcgcc
     7261 aacgggtcac acgcttctgc tactggatgc aacaggagcc tatcaccggg aaatggtgcg
     7321 ccagatgggg caaacgcatg atcatgtgtt gacgccaatg atgcaattgc aggacccgga
     7381 gaagacgaag gtgattatcg tgacgcttgc cgaaacgaca cctgtactgg aagcagcaaa
     7441 cctgcagcag gatttgcgtc gggccggaat tgaaccatgg gcatgggtaa tcaatagcag
     7501 tctggctgct gcgaaaccat cctcaccgtt cctcgtaacc cgagccagtc gcgaactgcc
     7561 gctgataaat gacgttacag agcagtttgc aaagcgtatt gcattaacac cactgcagaa
     7621 cgaagaacca gtgggtgcgg ctctgctggc aaaaatggca gactaataga tggggctgcg
     7681 gccccatctt ctgtagccaa acttttgctt atacagatta taagtttaag tgcttaacct
     7741 gctgtcgcga attccggtag tttgtaaaac accattactg gagcaattcg atgcacatta
     7801 tcaaagctga agaacagcac attaccgcga tacgcaatat ctatgctcac catgttctgc
     7861 atggtacagg tagcttcgaa accgaacctc cggatacgca ggaaatgctt aaccgactga
     7921 ggagcattca gtctcgagga ttcccatggt atgtcgccct gcagggcgat acgatcatcg
     7981 gctactgtta tatttcccgt taccgcgaac gccatgctta tcggttcacg gttgaaaatt
     8041 cgatatatat tgatccggtt tatcagcgac agggcgtcgg aaaagcactg ctcgatcatg
     8101 cattaaaatg ggccgaatct cagggatatc gtcagatgat tgctgttgtg ggtgacagcg
     8161 ctaacgtcgc ttctgttgcg ctgcatattc gcgcaggatt tactgaaata ggcacattaa
     8221 aggacattgg tttcaaacat ggccgctggc ttgacacggt tttgctgcaa atgaaacttg
     8281 gtgaagggaa tagtactctg ccagaagatt cagatcttat gctctgaggc aggaataagg
     8341 gggcgtctgc cccctttatg tttatttcaa tcgcttacct gtttcatcaa cgactttctc
     8401 gccgtcttcc ttagcaaatg cgcctttttg cgcatccgga agaatatcta gtaccacttc
     8461 ggaagggcgg caaaggcgcg ttcccaacgg tgtcaccaca atcgggcggt taatcaggat
     8521 cggatgctga agcataaaat cgattaactg atcgtcagta aacttgtctt cggcaagccc
     8581 cagttcttca taaggctcga catttttacg cagcagcgcc cgtactgtaa tccccatatc
     8641 tgcgataagt ttgaccaact catcgcgtga cggtggattt tcaaggtaat gaataacggt
     8701 cggttcatta ccgctgttgc ggatcatctc cagcgtatta cgcgacgtgc cgcaggccgg
     8761 gttgtgataa atggtgatgt tgctcatatc agtatctcat tacaaagtga aagagagacg
     8821 tagcgccagc gctgccagcg ttacaaacag cacaggcaga gtcatgatga tcccggtgcg
     8881 gaaatagtat ccccaggtga tggtcatatt cttctgtgaa aggacatgca gccaaagcag
     8941 cgttgccagg ctaccaatag gtgtaatttt aggtcccaga tcgcagccaa taacgttggc
     9001 ataaatcatc gcttctttga taacgcctgt tgcggtgctg ccatcaatag agagcgcgcc
     9061 aaccagcacg gtaggcatgt tgttcatgat ggaagacagg aaagccgtca ggaagccagt
     9121 accgagcgtc gcggcccaga gtcctttatc cgccagtaca ttcagcacac cagaaaggta
     9181 ttcggttagc ccggcgttgc gcagaccgta gaccaccaga tacatcccca gcgagaaaat
     9241 aacgatctgc cagggtgccc cacgcagcac tttaccggta ttaatggcat ggcctcgttt
     9301 agctactgca aacaggatca ccgctccaac agccgcaatc gcgctaacgg gaataccgag
     9361 cggctcaagg acaaagaacc caaccagcag aagaattaaa actatccagc cggttctgaa
     9421 ggttgccaga tctttaatcg cctttgccgg tgctttcagg agagccaggt cgtaagtcgg
     9481 tggaatatct ttgcggaaga acagatgcag catcaccagc gtggcgataa tagcggcaat
     9541 atccaccggc accattaccg acgcatactc agtgaagccc agtccaaaga agtccgccga
     9601 gactatattc accaggttcg acacgataag cggcaggctg gccgtatcgg caataaaacc
     9661 ggcagccatg acgaatgcca gcgtggtgcc tttgctgaac cctaatgcca gcagcatggc
     9721 gatgactatc ggcgtcagaa taagtgccgc accatcgttg gcaaacagtg ccgccaccgc
     9781 cgcaccgagc agaacgatat acgtaaagag caaacgtcca cgaccgttac cccagcggga
     9841 aacgtgcagc gctgcccatt cgaaaaaacc ggactcatca agcagcaggc tgataataat
     9901 tacggcaata aaggtcgccg tcgcgttcca gacgatattc cacaccaccg gaatatcgcc
     9961 cacatgcaca acgcctgata tcagagccag tcccgcgccc agcattgcac tccagccgat
    10021 ccctaatccc ttcggttgcc agataaccaa aacaatggtc aggacaaaaa tagcgcctgc
    10081 cagtaacata aaacctcctg acagggcgac tttgccgccc tgtatatgcc aaaaaattaa
    10141 ctcatcaact ctctgagctt ttcaataccg gcgggctctg acgccagcac cggaacaagc
    10201 gctatacggt cagcgtgctg attcttaaca gcctcaatct gaggctgttc ctgatgagcg
    10261 cgctggcaaa gcaatggaga acgcgtatcc gcaatggaaa ggctgttatt gataatccag
    10321 ccccacggat gaatccccgc tctttcaagg tcagcctgca ggtttgccgc ttccagtacc
    10381 ggtgtggttt caggcagagt gaccagcagg actttggtgc gttccgggtc ctgaagctgc
    10441 atcatcggag tggaaaaatg acctttatcc cccatcttct tggcaatctc acggtgataa
    10501 gccccggtag catcaagcag taatagcgta tgcccggtag gtgccgtatc catgaccaca
    10561 aaccgtttgc ctgcttcacg gattacgcgt gagaacgcct ggaacaccgc aatctcttcc
    10621 gtgcaaggag aacgtaaatc ctcttcgagc agccgtttcc ctgcctcatc cagatccctg
    10681 cctttcgtct caagaacatg ctgccgatag cgttcagttt catcgtgagg gttgattcgg
    10741 ctgacctgca gatttttgag acttccattg agcgttgcac tgagatgtgc agcgggatct
    10801 gaggtggtga gatggacatc aaatcccata tccgccagcc tgacggcgat ggcagcagcc
    10861 atcgtggttt tacctacgcc acctttgccc atcagcataa tcaaaccgtg ttcactacgg
    10921 gcgatatcat caaccaggcc agagagcgat aagttttcag gcgtgtacag gacgttcgtt
    10981 gccgggaggg gtaatctctc agagcaggtg tccagcaatc ccttcaatgc agacacgcca
    11041 accatattga ccggctggag taataaggta tctgtcggca actcagataa cccggaagga
    11101 agatttgcca acgcttcttg ttcccgttgc catatcgcag cggccagggc gtcatgttca
    11161 gcttcagttg taggcagcac accattaatc accaggtact ggtttttcag cccaatcgca
    11221 gccagttctt catgggtgcg ggcgacttcc tgcagcgtag atttttgcag tcgtgcaacc
    11281 agaaccagtc gggtacgatc aggatcggat aacgcctcta ctgcatgagc atactgctca
    11341 cgctgctttt ccagcccggc cattgggcca agacaggaag caccatccgg gttactttca
    11401 atgaagctac tccaggcacc gggaagctgg agaaggcgaa tcgtgtggcc cgtcggcgcg
    11461 gtatcaaaaa tgatgtgatc gaagcgggtc agcagggaag cgtctgtcaa taagccggtg
    11521 aactcgtcaa acgccgcaat ctcagtggta caggcccccg aaagctgctc gctgatgctg
    11581 ttaacaacgt catcaggcaa aagaccttta atagggtcaa cgattctgga gcggtattgt
    11641 ttggcggctt cctgcgggtc gatttccaga gcggaaagtc caggaactgc tgccacaggg
    11701 cgaatggtgt taccgatagc ctgatcgaat acctggccga cattggaagc cggatcggta
    11761 ctgaccagca aaacccgctt accctgttcg gcaaggcgga ttgccgtcgc gcaggaaata
    11821 gaagtttttc ctacacctcc cttaccggta aaaaacaggt aaggcgggat attctgtaag
    11881 aatttcatat gtcctcctga catactcagc aacaggaagt attaccacca cagcagctgg
    11941 tgggagctaa ccccactttc tccagcggta taccgaacca gcgagccagc tcagcgcgtt
    12001 ttgggtatcg ccctgccatc accgtttcac cgtccaacag cagtaacgga agcccttctg
    12061 ctccagatgc ttcgaggaat gctttcgctt tctcattctg aacaaagctc atgggctgct
    12121 gcgccaggtt gtaacgttca acctgtacac cacgtccttt cagccactgc acatcagcag
    12181 aaaaatcaac cagaacctga tcgacatctg agccacatac gccggtactg cagcacattg
    12241 ccgggtcaaa caccgttaac gtcttcattc tgaatacctc acattcgtaa aatcatatat
    12301 gttcaggcaa attttttaga tgcaaacagc cttaccgcta ccagagcagt ttgccgatgc
    12361 cagcttacgg gcgatggcct gtacatcgtc ctgttggctt aaccaggcct gctcaatcac
    12421 ctgagcagcc caggaaggaa tatgcgggga taagcgataa tgaacccact tgccctgttt
    12481 gcgatccagc aatagaccac tttcccggag cattgccaga tgacgggaga tcttgggctg
    12541 tgactgttct agcgcagtac aaaggtcgca cacgcacagc tctcccatct ctctaagcag
    12601 caacacgata cccaaacggg tttcatcgga gaggtttttg aagagttgta atgctgtaaa
    12661 aatcatagtc gttcccctac ctatctggct ctatgatact cggcgaaaga ttccttgcaa
    12721 gatcacatat ggtaaatcaa atgtatagca taaatagcga acgcacaagt ttgtgcgttc
    12781 gctattaact aaaggaatca ttcagtaagc cgacaagtat cgacaaaaat atcgaggttt
    12841 aaatccaaag gttttgataa aaatgttctc tcatgcttcc catcaccaat aaagaataaa
    12901 ttcgtaccat cagggaagta tcgattttta atatctttaa ccttggctct tcggaatgca
    12961 cttatctctt taccatcttt caagaggtta tcttcagcat actcagacat tgatataaat
    13021 gattgagtat cagtcggaag attttttccc acaaacgaca gtatgagatc aaaatttctg
    13081 tcatcgatat cttccttaag aattgcgtca agca
//
LOCUS       DAPHBJ010000121        13105 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.122, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000121 DAPHBJ010000000
VERSION     DAPHBJ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.122"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(97..309)
                     /gene="cspH"
                     /locus_tag="RHZ76_003071"
     CDS             complement(97..309)
                     /gene="cspH"
                     /locus_tag="RHZ76_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HDV2813668.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            595..807
                     /gene="cspG"
                     /locus_tag="RHZ76_003072"
     CDS             595..807
                     /gene="cspG"
                     /locus_tag="RHZ76_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HDV2813669.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            818..1006
                     /locus_tag="RHZ76_003073"
     CDS             818..1006
                     /locus_tag="RHZ76_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDV2813670.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            981..1211
                     /gene="ymcE"
                     /locus_tag="RHZ76_003074"
     CDS             981..1211
                     /gene="ymcE"
                     /locus_tag="RHZ76_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HDV2813671.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            1201..1374
                     /locus_tag="RHZ76_003075"
     CDS             1201..1374
                     /locus_tag="RHZ76_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HDV2813672.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1423..2496)
                     /locus_tag="RHZ76_003076"
     CDS             complement(1423..2496)
                     /locus_tag="RHZ76_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDV2813673.1"
                     /translation="MAENKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLVTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIGCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(2568..5312)
                     /gene="torS"
                     /locus_tag="RHZ76_003077"
     CDS             complement(2568..5312)
                     /gene="torS"
                     /locus_tag="RHZ76_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HDV2813674.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITIEMLKTSGAQIVAVGNAAQALETLQNSEPFAAALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMHIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5395..6423
                     /gene="torT"
                     /locus_tag="RHZ76_003078"
     CDS             5395..6423
                     /gene="torT"
                     /locus_tag="RHZ76_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HDV2813675.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6396..7088)
                     /gene="torR"
                     /locus_tag="RHZ76_003079"
     CDS             complement(6396..7088)
                     /gene="torR"
                     /locus_tag="RHZ76_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HDV2813676.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QSVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            7218..8390
                     /gene="torC"
                     /locus_tag="RHZ76_003080"
     CDS             7218..8390
                     /gene="torC"
                     /locus_tag="RHZ76_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HDV2813677.1"
                     /translation="MRKLWNALRRPSARWSVLSLVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            8390..10936
                     /gene="torA"
                     /locus_tag="RHZ76_003081"
     CDS             8390..10936
                     /gene="torA"
                     /locus_tag="RHZ76_003081"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDV2813678.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGLRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            10933..11532
                     /gene="torD"
                     /locus_tag="RHZ76_003082"
     CDS             10933..11532
                     /gene="torD"
                     /locus_tag="RHZ76_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDV2813679.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVARCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(11684..11989)
                     /gene="cbpM"
                     /locus_tag="RHZ76_003083"
     CDS             complement(11684..11989)
                     /gene="cbpM"
                     /locus_tag="RHZ76_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HDV2813680.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQDTTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11989..12909)
                     /gene="cbpA"
                     /locus_tag="RHZ76_003084"
     CDS             complement(11989..12909)
                     /gene="cbpA"
                     /locus_tag="RHZ76_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HDV2813681.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFSRQFQHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         3183 a         3340 c         3425 g         3157 t
ORIGIN      
        1 gtttctttgt ggaaaagtaa ctgatgttat aatgtatgat gttgactatt gcactattcc
       61 aataaaacca gtcagcttta aacaagcagc gtcatattaa gagagataaa catttgccgc
      121 tgttggtcct cgcaggccat ttacgcggca aaattccaca cgtaatcctg gtataagcac
      181 ttctgcgtcg cggggagtga atgcggaaat atggacctga acttctttac gaccgtcgga
      241 ggggataatg aatcctttgc cgcttttgcg atcaaaggtt ttgacaattc ctgtcatttt
      301 acgggacaaa caaattcctt actgaaaata ctgcgctaca ctatacgggg ttaataaaat
      361 aaagccagcg atatttaaga ccgccggacg gctaaaataa aatttgctta atctcaatta
      421 tcatgcgtta atagctgcgt cggtttgaaa gacagacagc atacaaagta gtttactaaa
      481 gcagttctca ttatcaggca ttatcccctt cttttgagtc tctctcctga acactaagta
      541 gtttctgtat taaagccctg tttgccgaaa ggcccaaaat gaaggaagta aaatatgtct
      601 aataaaatga ctggtttagt aaaatggttt aacgcagata aaggttttgg ctttatcact
      661 cctgatgatg gcagcaaaga cgttttcgtc catttcaccg ccatccagag caatgaattc
      721 cgcacgctga acgaaaatca gaaagttgaa ttttctattg agcaggggca acgtggcccc
      781 gcggcagcga acgttgttac gctctaaggt tgccattatt actcaacatc tccatttccg
      841 ctgtccatgt tgtcatggtt cacagtaccg cacatcggca ttcgatgtga cggagcgaaa
      901 ccctttgggc gctaagtgta ttttttgtaa atcgacgatg atcacctttg ataacgtcgc
      961 gctgcaaata cgcactgacc atgcgccgct ggatttcaca aaataatatc aggctccctc
     1021 gtggagcctt ttttatatct gccttatttt tcttcaacgc tgtatgtata gtaagcgata
     1081 acctgttgat tattgaatct ttcggggaga tggcttataa catttcttac ctgaccaggg
     1141 taccgggaac caacacctta ctggcgtgtt gctgtctttt aagaccagaa gaggttaaca
     1201 gtgaatattg aagagttaaa aaaacaagcc gaaacggaaa tcgccgactt tatcgcgcaa
     1261 aaaatcgccg agctgaacaa gaatacaggg aaagaagtct ctgaaattcg cttcaccgca
     1321 cgagaaaaaa tgaccgggct tgaaagttat gatgtcaaaa tcaaaataat gtgattttgt
     1381 gaacatcacc ccgtgcgagg tgatgttccg cttgttgcta atttagtgac caatcattgg
     1441 cgcttgtgga attaagcgtc ggtacaattc ctccggcacc gggctttgcc atactcccgc
     1501 atacattgcg taaccaatca ccgcgaacat aatccccaga accagtagcg tcattaacca
     1561 gccagacaac gcaaaagctt ttttatttgc cgcaggtttt tgcagtgaaa aggtcaatgt
     1621 tgaggctacc ggacatgact ctacgcaagt catacagccg gtacattcca ctgttcgtac
     1681 ctgaattaat ttatctaccg ggatccgtga tgggcaattt tttgcgcatt tgccacagcc
     1741 gatacaactt tcggcattgc gacgaatctt aaacggcgac aatagcgaaa ccacgcccat
     1801 cagcgcgcca tatgggcaaa gataacgaca ccaggcatgg cgaataaaca ggctggcaat
     1861 cagcaaaacg gtcacgctga ttaatgtcgc ggtccccata tgacgaaaga aatcgagcat
     1921 tttaacgtcc atcaccacgc tgtagggcga caacataaaa tagtgaatcg cctgagcggg
     1981 catcaataac gcgatataga gaaaaaaact caacagcaaa tacttcacgc cgcgcagagg
     2041 aatatccagc cagcggggaa ggacacattg ccgaccaaac agtttgttac cgagatcgcc
     2101 gattaattca gaaagcgtac caaccgggca taaccatgag caaaaggcct ttttgagtaa
     2161 tagactgatg acgataaaag cgaccaataa cagcatcgcg gcggcgtgga cggacggtaa
     2221 ctgacctgtt acaaggctat atttcagatt catcagcccg gcaatcggta gccagccttc
     2281 gatacctccc ggtctggcga caaatgtcgt gctacttgcc gtttcgtaat agcgcaccca
     2341 ataccagaac gtgatggcaa tataaatatt cattgccaac agtaataatt gcgtcgcttt
     2401 acgccaggtc gtggcattac gccagtcatt ccacggtaat ttgccgcccg tcgtgcctgg
     2461 ccgccgctgc cagcgggttc ttttattctc tgccatgatt ttgccagtcc gttaagttgt
     2521 ataccaaatg ccactattct agttgttctt aactggctga tattgattca aatcgcgttc
     2581 aggtctttct tatgcaacca tgcttccaga gcggcaacac tgcgtgtaat ttcttcgtgt
     2641 ggaagggggg cagataatgg ctgctgctcc agttgtgcgc atagctggct ggcgatatgc
     2701 attcccagac tcgagcaact gctttttagc tgatgcgcgg cacgctttat tttctcgcta
     2761 tcctgactgg cgcgggcaat gtcgatttca tcgagaagcg gcagggcatg ttgtgtaaat
     2821 aataccagcc attcgtggat cttctccgtc cccattaact gagcatcttc attgagttgc
     2881 gatacatcca gcgattgatc attattgact tgcagttgga gatagtgcgc cagtaactga
     2941 ccgagcactt cacgcggcac cggtttaggg ataatcccgc ggaataatga actggtacgc
     3001 tggcgcagcg tttcgtcaat gacatgggcg ctaaagccaa tcaaaaccag cgacggatat
     3061 tgctgtgcta gttgtcgggc aagcgtaatg ccgtcgatat ccggcagatc aaaatccacc
     3121 agtgcagcag caaacggttc gctattttgc agtgtctcta aagcctgcgc ggcattgcca
     3181 acagcaacaa tctgcgcacc actggttttc agcatctcaa tggtaattcg ctgggttagc
     3241 gggttatctt caattaacag caaacgtaaa ccgtcaagac gcaccgcctg attgactgtt
     3301 tttggcacgg gtgccgtggc aacacgtaac ggcaagcgta aacaaaaaca gcttccaacc
     3361 tccggcgtgc tggtggcgct cagttcgccg cccatcgcct gggccagacg gctactgata
     3421 gtcagtccca gcccggtgcc gccgcgtttg ccgcttacct gcacaaatgg ctggaagatt
     3481 tctgccagtt tcgcgggatc aataccgcag ccgctgtctt ccacttcgac cagccattgc
     3541 tcgccatcag tgcgactacg caggataatg tacccttcgt cagtaaaacg cagggcgttg
     3601 ctcaacaggt tggttataac ctgacgaata cgtcgtggat cgcccattaa cgcgcacggc
     3661 atatcatcgg caattgccgt tgccaggcga atcgggcgac ctttcacccg tccgctcatt
     3721 aattgcaggg tactttccag cagcgggcgc ggttcaaagg gctcatcgct gaccgaaaca
     3781 ttcttgccac ctgcttcgat agcggaataa tcgagaatat cgttgaggat ggtcagcaac
     3841 gattcaccag agtcagtaat tgcccgcaaa tcatcacgct gggcgttaag tgcggggtta
     3901 tctgccagca gttgagcagt gccgagaata ccgtacagcg gtgtgcggat ctcatggctc
     3961 atcgccgcca gaaacgccga ttttgcctgg ctggcttttt ctgcttccgc ccgtgcctgt
     4021 cggtgttcta tcaccagttc ctgcaattca gctgtacgcg ctttgacctg cgccgccagc
     4081 tgttcgcggt ggcgattcag tgcatgaaca ttgctgcgaa acgcatccat cagccgcccg
     4141 atggtatcca gctcccgtac gccagcggtt tccgggaaag gggagtcaat atcaccgtcc
     4201 agcagccgtt gcagcgcctg cgtttgttcg gcaagtggac gcgtgactga gcgataaacc
     4261 acgcgccaga ggatcagaat cagtgcgcaa agtgaaacca tccccagcaa taacaggctg
     4321 tattgcccgc gtgcactcgc tttttccaga tgcgccagtc cgtgctgatt acgcagctca
     4381 atggtgtcga ccagctgact gacttcgcta ctaaactgcg cgaactgggc gatgttattt
     4441 tgtgcgagag tttgtaggtg attgctgatt tcactgtcct gctgatacag cgccagcaaa
     4501 tcgctatatt ggctaacggt agttaacgtt gttgcgacct gcgcacgaac acccggatct
     4561 tcaatgcgta tttgccgacg ttgcagaatt ttcaccgcat tattgagctg cttttccagc
     4621 gttggtgcat ttttctggat ctgctccagc cccagattca tcaccatttg ctgcacccgc
     4681 agagcgctaa ggcgcagttc attcatctgg ttaacatact caagatcgat atcaatcagc
     4741 cgatcgagtg cactttcagc agcctgacgc tgatcttgtt cgatcaaatc gtaaatcccg
     4801 gcctgggtcg ctccagcgga agttgtcgca ttattcgcct gaccttgcgc caggcgtgcg
     4861 atctcatcgg cggcagcgac tatctgctga ctgagttgct gttgttgctg gcgcagttgc
     4921 aaacgctgcc ccaccagttc cccttgctga cgtaacgaac gggagatctc ctgctcctgt
     4981 tgttcaatag cggtggtatc aaaaccttgt tcccgtaacg cttgcagcaa cgcattaatc
     5041 ttcaggcttt gtgcggtgag cattcgcccc tgcgcctgcc acatcttttc gttatcggca
     5101 ctggtcaggt tctgcgcggc gaaaagttcc caggcgctgg cttcgctcaa ctggcgcgcc
     5161 atattcatgg taggaatcaa tgcctgagtg ttgtcttttt ccacctggct gataaagcgc
     5221 aggttgtacc atcccaccag ggtactggtc agggttaaca gcgccatcag ggcaaagccc
     5281 atccagagtc ttcgggtcag ggttaaattc acggtcggtg cactttaggt gaaaaaggtt
     5341 gagtcgcaaa gcggaatgca tctagcataa agccttatta ttgatgaggc tatcatgcgc
     5401 gtactgctat ttttacttct ttcccttttc atgttgccgg cattttcggc tgataacctg
     5461 ttgcgctggc atgatgcgca gcatttcacg gtgcaagcct ctacgccgct taaagccaaa
     5521 cgcgcatgga aactgtgcgc gctttatccc agcctgaaag attcatattg gttatcgttg
     5581 aactatggta tgcaggaggc tgctcgccgc tacggtgtgg atttaaaagt gctggaggca
     5641 ggcggctaca gccagttggc tacccagcaa gcacaaatcg accagtgtaa acagtggggc
     5701 gcagaggcca ttttgctcgg tagtagcacg acctcatttc ccgacctgca aaagcaggta
     5761 gcaagtctgc cggtgatcga actggtaaat gctattgatg ctccccaggt gaaaagccgc
     5821 gttggtgtgc cctggtttca gatgggctat caaccggggc gatatctggt gcaatgggcg
     5881 cacggtaaac cactgaatgt gctgttgatg cccggacccg ataacgccgg gggcagtaag
     5941 gagatggtcg agggttttcg cgcagccatt gccggaagcc cggtgcgtat tgttgatatt
     6001 gcgcttggtg ataacgatat tgaaatccag cgtaacctgt tgcaggagat gctggaacgc
     6061 catccagaaa tcgacgtcgt tgccggaacg gccattgcgg cagaggcggc aatgggggaa
     6121 gggcgtaacc tgaaaacgcc gcttaccgtg gtgtcgtttt atctttcaca tcaggtgtat
     6181 cgcgggctga agcggggaag agtgattatg gctgccagcg atcaaatggt ctggcagggg
     6241 gaactggcgg ttgagcaggc catcaggcaa ttacaggggc aatcggtgtc tgataatgtc
     6301 agcccaccga ttttagttct gacgccgaaa aatgccgacc gtgaacatat tcgccgctcg
     6361 ctgtcaccag ggggatttcg tccggtctat ttttatcagc acacatcagc ggctaagaaa
     6421 taaccttcac catgttgcgt caccagtaaa tccgcgctga gtttatgacg taaacgacga
     6481 attaacacat cgacggtgcg caggtcaggg ttttccaccc gacgcgcaga aagcatacgt
     6541 agcagacgtt cacggctgag aatttcgccc ggattcgtca caaatgccac caacatttca
     6601 tactctgcgc gggtcagttt aatcggctcg ccatcccgct ccagcgtatg gcgcgacaca
     6661 ttcaggcaat aaccggcaaa gcgatagcag ttgtcctgag tgtgcggttg agcttgtcgc
     6721 gcgaggtcga ttcgccagag cagatttttc acccgtacta ccagttcgcg cagttccagc
     6781 ggtttggtga cgtaatcgtc tgcgcccatt tccagcccaa caatacggtc aatccgatcg
     6841 ctgcgtccgg taaccagaat aatccccacc gttgagcgtt ctcgcagggc gcgggttaac
     6901 atcaggccat tttcatcggg taagttgata tccagcagaa ttaaatctac cgactgattc
     6961 tgcataattt cccgtagccc ggcaccgctc gccgtaacgg aaacggtata cccctcctga
     7021 gtgaagtagg attgtaatcg cgcctgggta accggctcat cttcaacaat aacaatgtga
     7081 tgtggcatca gagggtttta ctcattctgt tcatatctgt tcatattctg ccgtaagccg
     7141 ttcatcctga ccagtgccgc tgttcatatt tgctcattaa gatcgcttca ctaaaccata
     7201 attctacagg ggttattatg cggaaactct ggaacgcgct acgccgaccc agtgctcgtt
     7261 ggtcggtact gtcgctggtc gcaattggga ttgtgattgg cattgcgctg attgtattgc
     7321 cccacgttgg gatcaaagtc accagcacaa ccgaattttg tgtcagttgc cacagtatgc
     7381 aaccggtgta tgaagaatat aaacagtcgg tgcatttcca gaacgcctcc ggcgtgcgag
     7441 ctgaatgcca tgactgtcat atcccgccgg atattccagg catggtgaag cgcaaactgg
     7501 aagcgagcaa tgatatctac cagaccttta ttgctcactc cattgataca cctgaaaaat
     7561 tcgaagccaa acgcgcggaa cttgccgagc gtgaatgggc gcgaatgaaa gaaaacaact
     7621 cggcaacctg ccgctcctgc cataactacg atgcgatgga tcatgcgaag cagcatcctg
     7681 aagcagcacg tcagatgaag gtggcagcga aagataatca atcctgcatc gactgtcata
     7741 aaggtattgc ccaccagtta ccggatatga gtagcggctt ccgtaagcag ttcgatgagc
     7801 tgcgcgccag tgctaatgac agtggtgaca cgctgtactc tattgatatt aagccaattt
     7861 atgcggcgaa aggcgataaa gaagcctctg gttctctgct gcctgcttcg gaagtgaaag
     7921 tccttaaacg tgacggcgac tggctgcaaa ttgaaattac cggctggacg gaaagcgccg
     7981 gacgtcagcg tgtactcacc caattcccag gtaaacgcat ctttgttgcc tcgattcgtg
     8041 gtgatgtgca gcagcaggta aaaacgctgg agaaaaccac cgttgccgac accaataccg
     8101 agtggagcaa gttgcaggcc actgcgtgga tgaagaaagg cgacatggtg aacgatatca
     8161 aaccgatctg ggcttatgcg gattcgttgt acaacggcac ctgtaaccag tgccacggcg
     8221 caccggaaat cgcccacttt gacgctaacg gttggatcgg cacgctcaac ggcatgattg
     8281 gctttaccag tctcgataaa cgtgaagaac gcaccttgtt gaaatatctg caaatgaatg
     8341 cgtctgacac cgcaggtaag gctcacggcg ataagaagga agaaaaataa tgaacaataa
     8401 cgatctcttt caggcatcac gtcggcgttt tctggcacaa ctcggcggct taaccgtcgc
     8461 cgggatgctg gggccgtcat tgttaacgcc gcgacgtgcg actgcggcgc aagcggcgac
     8521 tgacgctgtc atctcgaaag agggcattct taccgggtcg cactgggggg ctatccgcgc
     8581 gacggtgaag gatggtcgct ttgtggcggc aaaaccgttc gaactggata aatatccgtc
     8641 gaaaatgatt gccggattgc cggatcacgt acacaacgcg gcgcgtattc gttatccgat
     8701 ggtacgcgtg gactggctgc gtaagcgcca tctcagcgat acctcccagc gcggtgataa
     8761 ccgttttgtg cgcgtgagct gggatgaagc cctcgacatg ttctatgaag aactggaacg
     8821 cgtgcagaaa actcacgggc cgagtgcctt gctgaccgcc agtggttggc aatcgacggg
     8881 gatgttccat aacgcttcgg ggatgctggc gaaagctatt gccttgcatg gtaatagcgt
     8941 tggtacgggc ggagattact ctaccggtgc tgcgcaggtg atcctgccgc gcgtagtcgg
     9001 ttcgatggaa gtgtatgaac agcaaacctc ctggccgctg gtattgcaga acagcaaaac
     9061 cattgtgctg tggggctccg atttgctgaa aaaccagcaa gcgaactggt ggtgcccgga
     9121 tcacgatgtt tatgaatatt acgcgcagct aaaagcgaaa gtcgccgccg gtgaaattga
     9181 ggtcatcagc atcgatccgg ttgtcacatc cacccatgag tatctggggc gcgagcatgt
     9241 gaagcacatt gcggttaacc cgcaaactga cgtgccgctg caactggcgc tggcacatac
     9301 gctgtacagt gaaaacctgt acgacaaaaa cttccttgct aactactgtg tgggttttga
     9361 gcagttcctg ccgtatctgc tgggtgagaa agacggtcag ccgaaagatg ccgcatgggc
     9421 tgaaaaactg accggcattg atgccgaaac cattcgtggg ctggcgcggc agatggcggc
     9481 gaacagaacg caaattattg ctggctggtg cgtgcagcgt atgcagcacg gtgaacagtg
     9541 ggcgtggatg attgtggttc tggcggcgat gctggggcaa attggcctgc caggtggtgg
     9601 ttttggtttt ggctggcact acaacggcgc aggcacgccg gggcgtaaag gcgttattct
     9661 gagtggtttc tccggctcta cgtcgattcc gcctgttcac gacaacagtg actacaaagg
     9721 ctacagcagc actattccga ttgcccgttt tatcgatgcg atcctcgaac cggggaaagt
     9781 gatcaactgg aacggtaaat cggtaaaact gccgccgctg aaaatgtgta tttttgccgg
     9841 aactaaccca ttccatcgcc atcagcagat caaccgcatt attgaaggct tgcgcaagct
     9901 ggaaacggtt atcgccatag ataaccagtg gacctcaacc tgccgctttg ccgatatcgt
     9961 actgcctgcg accacgcagt ttgagcgtaa cgatctcgac cagtacggca atcactccaa
    10021 ccgtggcatt atcgccatga aacaggtggt gccgccgcag ttcgaggcgc gcaacgactt
    10081 cgatattttc cgcgagctgt gccgtcgctt taatcgcgaa gaagccttta ccgaagggct
    10141 ggacgaaatg ggctggctga aacgcatctg gcaggaaggt gtacagcaag gcaaaggacg
    10201 cggcgttcat ctgccagcgt ttgatgactt ctggaataac aaagagtacg tcgagtttga
    10261 ccatccgcag atgtttgttc gccaccaggc attccgcgaa gatccggatc tcgaaccgct
    10321 gggcacgccg agtggcctga ttgagatcta ctcgaaaact atcgccgata tgaactacga
    10381 cgattgtcag gggcatccga tgtggtttga gaaaatcgaa cgctcccacg gtgggcctgg
    10441 ctcgcaaaag tatccgttgc atctgcaatc tgtgcatccg gatttccgac ttcactcgca
    10501 gttatgtgag tcggaaacgc tgcgtcagca atatacggta gcgggtaaag agccagtatt
    10561 cattaacccg caggatgcca gcgcgcgcgg tattcgtaac ggtgatgtgg tacgcgtctt
    10621 taacgctcgc ggtcaggtgt tggcaggggc agtggtttct gaccgctatg cacccggcgt
    10681 ggcacgaatt cacgaagggg catggtacga tccagataaa ggcggcgagc ctggtgcgct
    10741 gtgcaaatac ggtaacccca acgtgttgac catcgacatc ggtacatcgc agctggcgca
    10801 ggcgaccagt gcgcacacta cgctggtgga aattgagaag tacaacggaa cagtggagca
    10861 ggtgacggcg tttaacggcc ccgtggagat ggtggcgcag tgcgaatatg ttcccgcgtc
    10921 gcaggtgaaa tcatgaccac gctgacagca caacagattg cctgtgttta cgcctggcta
    10981 gcgcagttgt tctcccgtga gctggacgat gaacaactga cgcaaatcgc cagtgcgcag
    11041 atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc tggctgcggc ggtgaatgag
    11101 ctggaaaact gtattgccac gctgacagta cgtgacgatg cccgtctgga actggccgct
    11161 gacttttgcg gcctgtttct gatgaccgac aaacaagcgg cgctgccgta tgcatcggcc
    11221 tacaaacagg acgagcaaga gattaaacgc ttgttagttg aggcagggat ggaaaccagc
    11281 ggcaatttca acgaaccggc agatcatctg gcgatctatc tcgaattgct cagccatctg
    11341 catttttcgc tgggagaggg gaccgttcct gcgcgaagaa tcgacagttt gcggcaaaaa
    11401 acactgacgg cgctgtggca atggttacca gagtttgttg cgcgttgtcg tcagtatgac
    11461 agctttggtt tttacgcggc actaagccag ttattgctgg tgttagtgga gtgcgaccac
    11521 caaaacagat aacgtcgttt gtgcgcctga aaagacgcgt ttagcgtcgc atcaggcatt
    11581 atggcgcagt tgccggatgc ggcgtgaacg tcttatccgg cccacaggaa ctgtaatctt
    11641 tgtagaccgg ttaagatgcg tcatcgcatc cggcaaacac acatcacgga tgagctacaa
    11701 accgggaaag ccgctggcgc agcaggcggt tttcctgctt caggtgcgca atatcatcca
    11761 ttaacgtcag cgccaccgcg atccccggcc agtccagagc cagttcatga cgcaggcgta
    11821 ccgcgcgttg caccacaatg gcggcatggt cgtcaaatac ccaggttgtg tcctggatct
    11881 cacgcggttc aaccaccccc aaaccgacaa tttcattcaa ctcctcttca gagatgccgg
    11941 tatgcaggca aaattcggta atagtaaaag tcaccgtaac attagccatt atgctttccc
    12001 ccaatcttta cgtggatcaa aagacgactg ggcgtctgcc agttgctgcc acagcgcggc
    12061 agtgttttca tccggtttcg gcggcatcac gattttcagt accgcataca gatcgccggt
    12121 ctgttttttg ctcaccagac ctttgccttt aacgcgcaat cgttgcccgg cctggctgcc
    12181 tggcgggata gtcagcaaaa tgctttcttt cagtgttgga acggtgactt tagcacccag
    12241 cgccgcttcc cacgggctaa ccggcaccac aatttccaga tcctggccga caatatcaaa
    12301 cagcggatgt ggcgcaatat gaatcaccag ccacaaatcg ccatttggac cgccgttttc
    12361 gcccggcgtc ccctggcctt tcaggcggat gcgttgacca ttgccgacgc ccgccgggat
    12421 cttcacattc agcgttttcg ggatttcctg ttcgatcatg ccaaaggcgt tataaaccgg
    12481 caggttatag ctgatggtac gcttatgctc agtaagcgtt tcttcgagga ataccgccac
    12541 ttcgatttca atatcgtggc cgcgtgtggc ggggcgttga cggctctggc gggcatgctg
    12601 accgaaaatt gacgagaaga tatcgtcaaa atcttcggcg ttaaaactct gaccgtctcc
    12661 atgctgaaac tgacggctaa attgtggatc gttgcgatgt tgccacatct gatcatactc
    12721 agcgcgacgt tgttcatcac tcaacacttc ccaggcttct gcaacctctt tgaagcgggc
    12781 ttcggcatcc ggttctttgc tgacatcagg atggtatttg cgggccagtc gacgataggc
    12841 ggtcttgatt gtcttgagat cgtccgtcgg tttcacgccc atgatggcgt aataatcctt
    12901 taattccata gcgttatctc gcgtaaatca acacaaattg aaggaacccc tgtaaggtaa
    12961 ctcctataag tgtagggtaa tcctcaaaat ttcatatgcc aacacagaat atgttattga
    13021 aatcatcgcg gagaggaggt cgcctcaagg tggggtgttg aacatatttt aaacaggtga
    13081 aaaagggtga gcgatttttg atagt
//
LOCUS       DAPHBJ010000122        13093 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.123, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000122 DAPHBJ010000000
VERSION     DAPHBJ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.123"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            423..2054
                     /gene="oppA"
                     /locus_tag="RHZ76_003085"
     CDS             423..2054
                     /gene="oppA"
                     /locus_tag="RHZ76_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HDV2813682.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2140..3060
                     /gene="oppB"
                     /locus_tag="RHZ76_003086"
     CDS             2140..3060
                     /gene="oppB"
                     /locus_tag="RHZ76_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HDV2813683.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3075..3983
                     /gene="oppC"
                     /locus_tag="RHZ76_003087"
     CDS             3075..3983
                     /gene="oppC"
                     /locus_tag="RHZ76_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HDV2813684.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3995..5008
                     /gene="oppD"
                     /locus_tag="RHZ76_003088"
     CDS             3995..5008
                     /gene="oppD"
                     /locus_tag="RHZ76_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HDV2813685.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            5005..6009
                     /gene="oppF"
                     /locus_tag="RHZ76_003089"
     CDS             5005..6009
                     /gene="oppF"
                     /locus_tag="RHZ76_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HDV2813686.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6062..6391)
                     /locus_tag="RHZ76_003090"
     CDS             complement(6062..6391)
                     /locus_tag="RHZ76_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HDV2813687.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6426..7886)
                     /gene="cls"
                     /locus_tag="RHZ76_003091"
     CDS             complement(6426..7886)
                     /gene="cls"
                     /locus_tag="RHZ76_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDV2813688.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8029..8202
                     /locus_tag="RHZ76_003092"
     CDS             8029..8202
                     /locus_tag="RHZ76_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HDV2813689.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8257..9510)
                     /gene="kch"
                     /locus_tag="RHZ76_003093"
     CDS             complement(8257..9510)
                     /gene="kch"
                     /locus_tag="RHZ76_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HDV2813690.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(9810..10106)
                     /locus_tag="RHZ76_003094"
     CDS             complement(9810..10106)
                     /locus_tag="RHZ76_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HDV2813691.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10330..11049
                     /gene="tonB"
                     /locus_tag="RHZ76_003095"
     CDS             10330..11049
                     /gene="tonB"
                     /locus_tag="RHZ76_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HDV2813692.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVTPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(11089..11487)
                     /gene="yciA"
                     /locus_tag="RHZ76_003096"
     CDS             complement(11089..11487)
                     /gene="yciA"
                     /locus_tag="RHZ76_003096"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HDV2813693.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(11592..12131)
                     /locus_tag="RHZ76_003097"
     CDS             complement(11592..12131)
                     /locus_tag="RHZ76_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HDV2813694.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(12161..12904)
                     /locus_tag="RHZ76_003098"
     CDS             complement(12161..12904)
                     /locus_tag="RHZ76_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HDV2813695.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(12932..13021)
                     /locus_tag="RHZ76_003099"
                     /pseudo
     CDS             complement(12932..13021)
                     /locus_tag="RHZ76_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
BASE COUNT         3509 a         3107 c         3217 g         3260 t
ORIGIN      
        1 acatgcttag ccaatctcta tttgattgaa ttgaaagatg tttgttaagg catggatgca
       61 agctatagat tctgatacgg tcaataaaag agaattgctt aacaattttg caaaatgtat
      121 tggcgagtaa gaaccgcatt tggtactttc cgggcaaccg ccagacgatt ctttattggt
      181 aatgagaata attaacaatt aaagagcgtc gcgaaagaat aatgtgtctc gacaggggag
      241 acacagtacg aatcgacata aggtgatcgt ctgaatcacc agaataaata aagtcggtga
      301 tagtaatacg taacgataaa gtaacctgac agcagaaagt ctccgagcct gtgcagggtc
      361 ccaatccggg attacacatg ctggttaata ccagtaatta taatgaggga gtccaaaaaa
      421 caatgaccaa catcaccaag agaagtttag tagcagctgg cgttctggct gcgctaatgg
      481 cagggaatgt cgcgctggca gctgatgtac ccgcaggcgt cacactggcg gaaaaacaaa
      541 cactggtacg taacaatggt tcagaagttc agtcattaga tccgcacaaa attgaaggtg
      601 ttccggagtc taatatcagc cgagacctgt ttgaaggctt actggtcagc gatcttgacg
      661 gtcatccagc acctggcgtc gctgaatcct gggataataa agacgcgaaa gtctggacct
      721 tccatttgcg taaagatgcg aaatggtctg atggcacgcc agtcacagca caagactttg
      781 tgtatagctg gcaacgttct gttgatccga acactgcttc tccgtatgcc agttatctgc
      841 aatatgggca tatcgccggt attgatgaaa ttcttgaagg gaaaaaaccg attaccgatc
      901 tcggcgtgaa agctattgat gatcacacat tagaagtcac cttaagtgaa cccgttccgt
      961 acttctataa attacttgtt cacccatcaa cttcaccggt gccaaaagcc gctatcgaga
     1021 aattcggcga aaaatggacc cagcctggta atatcgtcac caacggtgcc tataccttaa
     1081 aagattgggt cgtaaacgaa cgaatcgttc ttgaacgcag cccgacctac tggaacaacg
     1141 cgaaaaccgt tattaaccag gtaacctatt tgcctattgc ttctgaagtt accgatgtca
     1201 accgctaccg tagtggtgaa atcgacatga cttataacaa catgccgatc gaattgttcc
     1261 agaagctgaa aaaagagatc ccggacgaag ttcacgttga tccatacctg tgcacttact
     1321 attacgaaat taacaaccag aaaccgccat tcaacgatgt gcgtgtgcgt accgcactga
     1381 aactaggtat ggaccgcgat atcattgtta ataaagtgaa agcgcagggc gacatgcccg
     1441 cctatggtta cactccaccg tatactgatg gcgcaaaatt gactcagccg gaatggtttg
     1501 gctggagcca ggaaaaacgt aacgaagaag cgaaaaaact gctggctgaa gcgggttata
     1561 ccgcagacaa accgttgacc atcaacctgt tgtataacac ctccgatctg cataaaaagc
     1621 tggcgattgc tgcctcttca ttgtggaaga aaaacattgg tgtaaacgtc aaactggtta
     1681 accaggagtg gaaaacgttc ctcgacaccc gtcaccaggg tacttttgat gtggcccgtg
     1741 caggctggtg tgctgactac aacgaaccaa cttccttcct gaacaccatg ctttcgaaca
     1801 gctcgatgaa taccgcgcat tataagagcc cggcctttga cagcattatg gcggaaacgc
     1861 tgaaagtgac tgacgaggcg cagcgcacag ctctgtacac taaagcagaa caacagctgg
     1921 ataaggattc ggccattgtt cctgtttatt actacgtgaa tgcgcgtctg gtgaaaccgt
     1981 gggttggtgg ctataccggc aaagatccgc tggataatac ctatacccgg aatatgtaca
     2041 ttgtgaagca ctaatggcaa tacgtggggc aggagtgtcc tgctccacgg tgtctgattt
     2101 ttatcgcatt acagaaggca caggccagaa ggtagggcaa tgttaaaatt tattctacgt
     2161 cgctgtctgg aagcgattcc gacgctattt attcttatta ctatttcgtt ctttatgatg
     2221 cgcctcgcgc cgggaagccc ttttaccggc gaacgtactt taccgccaga agtgatggcc
     2281 aatatcgaag cgaaatatca tcttaatgat ccgatcatga cacagtattt cagctacctg
     2341 aaacaactgg cgcacggtga tttcggtcca tcgtttaaat ataaagatta ttcggtcaat
     2401 gacttggttg catccagttt tcccgtttct gccaaactgg gagccgcagc atttttcctt
     2461 gcggtaatac tgggtgttag tgctggcgtt attgccgcat taaaacaaaa caccaaatgg
     2521 gactataccg tgatggggct ggcaatgacc ggggttgtta tccccagttt tgtggttgca
     2581 ccattattag tcatgatatt tgcgatcatt ttgcattggc tgccgggcgg tggctggaat
     2641 ggtggggcgc ttaaattcat gatattgccg atggtggcgt tgtcactcgc ttatatcgcc
     2701 agtattgcgc gtattacccg tggctctatg attgaagtat tacactccaa ttttattcgt
     2761 actgcccggg cgaaagggtt acctatgcgg cggatcattt tacgtcacgc attaaaacct
     2821 gctctgttac ccgtgctctc ttatatgggc cccgcatttg tcggcattat taccggttct
     2881 atggttatcg aaaccattta tggtttgccg gggattggac aactgttcgt taatggcgca
     2941 ttgaaccgtg actattcctt agtgttaagc ctgaccatcc tggttggtgc tttaaccatt
     3001 ttgtttaatg ccattgtcga tgtgctatat gcggttatcg acccgaaaat ccgttactga
     3061 tactggagct cgcgatgatg ttaagtaaga aaaacagcga gacgctggaa aatttcagtg
     3121 aaaagctgga ggtcgaaggg cgcagcttgt ggcaggacgc acgtcgacgt tttatgcata
     3181 accgtgcggc ggttgccagt ctgatagtgc tggtgctgat cgcgttattt gtaatcctgg
     3241 caccgatgct ttcccagttt gcctatgacg atactgactg ggcgatgatg tccagcgccc
     3301 cggatatgga gtccggtcac tactttggta ctgactcatc cggtcgcgac ctgcttgtgc
     3361 gcgttgcgat tggcgggcgt atctcactca tggtcggtgt tgctgcggca ctggtggctg
     3421 tggtcgtcgg gacactttat ggttcgcttt ccggttatct gggcggtaaa gtggattcgg
     3481 taatgatgcg tctgctggaa atcctcaact ccttcccatt catgttcttc gtcattttgc
     3541 tggtgacctt tttcggtcaa aacatcctgc tgattttcgt ggcgataggc atggtttcct
     3601 ggctggatat ggctcgtatt gtgcgtgggc aaaccctgag tctgaagcgc aaagagttta
     3661 ttgaggcggc acaagttggc ggtgtatcga catcgggcat tgttattcgc cacattgtgc
     3721 cgaatgtact cggtgtggtg gtggtctacg catcacttct ggtgcccagc atgatcctct
     3781 ttgaatcttt ccttagcttc ctggggttgg gtacgcaaga gccgttaagc agctggggtg
     3841 cattgctgag tgatggcgcg aactcgatgg aagtctctcc atggttattg ttgttcccag
     3901 cgggattcct cgtggtgacg ctgttttgtt tcaactttat cggcgatggc ttgcgtgatg
     3961 ccctcgaccc gaaagatcgt taaggagtgc agccatgagc gtaattgaaa ctgcaactgt
     4021 gccgctcgca caacaacagg ctgacgcact gctgaacgtg aaagatttgc gtgtcacctt
     4081 tagtaccccg gacggcgacg tcacggcggt caatgatttg aatttttccc tacgtgccgg
     4141 agaaacgctg ggtattgtag gtgagtctgg ttcgggtaaa tcgcaaactg catttgcgtt
     4201 gatgggcctg ttggctgcca acggacgtat tggcggatcg gcaaccttca atgggcgtga
     4261 aatcctcaat ttgccagagc atgaactcaa taaactgcgc gctgaacaaa tctcaatgat
     4321 ttttcaggac ccaatgactt cgttgaatcc ctatatgcgc gtcggtgagc agttaatgga
     4381 agtgctgatg ctgcataaga acatgagcaa agctgaggcg tttgaagagt cggtgcggat
     4441 gctcgatgcg gtaaaaatgc cggaagcgcg taaacgcatg aaaatgtacc cgcacgaatt
     4501 ttctggcggc atgcgtcagc gagtcatgat tgcgatggca ttgctatgtc gacctaagct
     4561 actgattgcg gatgaaccca ctactgcgct ggacgtcacc gtacaggcgc agatcatgac
     4621 gctattaaat gaactgaagc gggaatttaa taccgccatc attatgatca cccacgatct
     4681 tggcgtggtg gcggggatct gcgacaaagt gctggtaatg tacgccgggc gcacgatgga
     4741 atatggcaat gcgcgcgatg tcttttatca acccgttcat ccttattcta tcggtttgct
     4801 caacgcggtg ccgcgtcttg atgcggaagg tgaaacaatg ttgaccatcc ctggtaatcc
     4861 gccaaacttg ctgcgattac cgaaaggttg cccgttccag ccacgttgtc cacatgcgat
     4921 ggaaatttgt agtagcgcac cgccgctgga agagtttacg cctggccgtc tgcgtgcttg
     4981 ctttaaaccg gtggaggaac tgttatgaat gctgtaactg aaggaagaaa agtcctcctt
     5041 gaaatcgccg atcttaaagt gcactttgaa atcaaagatg gcaaacagtg gttctggcaa
     5101 ccgccgaaaa cgctcaaagc cgtcgatggt gtcactcttc gcctgtatga aggggaaaca
     5161 ttaggtgtgg taggggaatc gggatgcggt aagtccacct ttgctcgcgc catcatcggt
     5221 ttggtcaagg cgaccgacgg tcatgttgcc tggttaggta aagagttgct gggcatgaag
     5281 cccgatgaat ggcgtgccgt tcgcagtgat attcagatga ttttccagga tccgttggca
     5341 tcgctaaacc cgcgtatgac catcggcgag atcatcgctg aaccactgcg tacttatcat
     5401 ccgaaaatgt cacgccagga agttcgcgag cgcgtgaagg cgatgatgct gaaagtcggg
     5461 ttattgccta acctgattaa ccgctatccg catgagttct ccggtgggca gtgccagcgt
     5521 atcgggattg ctcgtgctct tattcttgaa ccgaagctga ttatctgcga tgagccggtg
     5581 tcggcgctgg acgtgtcaat tcaggcgcag gtggtcaacc tgctccagca gctgcaacgt
     5641 gagatgggat tgtcattaat ttttatcgct catgacctgg ccgtggtaaa acacatttcc
     5701 gatcgtgtgt tggtgatgta tctcggccat gcggtagaac tggggaccta tgatgaggtc
     5761 taccacaatc cactacatcc ttacaccagg gcattgatgt cggcagtccc catacctgat
     5821 ccggatctgg agaagaacaa aaccatccag ttactggaag gggaattacc gtcgccgatc
     5881 aacccgcctt ccggttgtgt tttccgtacc cgttgcccga ttgccggtcc ggagtgcgcc
     5941 aaaacacgtc ctgttctgga ggggagtttc agacacgccg tttcttgcct gaaagtcgat
     6001 ccgctttaaa cacaataagg gctgacagtt gtcagccctt tttcacgccg aaagcgatta
     6061 tttattcccg ccagatgata tggcaaagtt tgtgatcttt ttcgcggcat aacagaatgc
     6121 gggcgaaaac atcgttgatt tcaccatctt cactgtccgc cagaccaatc accacttcgg
     6181 caaaaaagtc cgggttcaaa tcgaaatcaa catgttcctg ccagtcttcc gccggatcga
     6241 ataactccgc gccgccgcgc tcttcaaact gaagattgaa cagcagaaca tcagctggat
     6301 cgagattgtc ggcagccagt tcgagaaaaa tatcgtaggc ctgctcaagc gtttcatctt
     6361 cagtcaggcg attgtttaga tccatatcca tagtcactac ctgtttaacc tctgttggcg
     6421 acgttttaca gcaacgggct gaagaagtaa aacagtcgct cggcgacacg ttgccataat
     6481 ggacgtttta gccataaacg ggcatcgagc agacgtgaac gcgaaatata atcgtcctga
     6541 acggcggcaa ggtctgcacc aaaaccttta tcgtcgattg ccagagtaat ctcgaaattt
     6601 agccacagac tccgcatatc aaggttaacg gtgccaacca gacttagttc accatcgacc
     6661 agcacgctct tggtatgcag taacccgcct tcaaactgat aaattttaac cccagcagcc
     6721 agcagttccg taaagaatgc gcgactggcc cagccgacca gcatcgagtc attttttcgc
     6781 ggaaggataa tactgacatc caccccgcgc tgtgccgccg tgcaaatcgc atgaagtaaa
     6841 tcatcgcttg gcacaaagta gggcgttgtc atgatcaaat attcacgcgc cgaataagcc
     6901 gcagtcaata atgcctggtg aatgagatct tccggaaagc ctgggccaga agcaattgtg
     6961 tgaatggtgt gaccgctggc ctgttcaaac ggcataatat tgacatctgg tggtggcggc
     7021 agaatacgtt ttccggtttc aatctcccag tcgcaggaat aaataatccc catcgcggtg
     7081 gcgatggggc cttccatacg cgccatcaga tcaatccatt gccctacgcc cgcatcttgt
     7141 ttgaagtagc gagggtcgac catattcatg ctgccggtgt acgcgatgta attatcgatc
     7201 atgatcatct tgcgatgttg gcgcaggtcc atacggcgta aaaacacacg catcagattg
     7261 acctttaagg cttcgaccac ttcaataccg gcattacgca ttagctcggg ccacgggctg
     7321 cggaaaaaag ccacactccc ggcggagtcg agcatcaatc ggcaatgaat gccgcgtcgc
     7381 gcagcggcca ttaatgattc agccacctgg tccgccatgc cgccgggctg ccagatataa
     7441 aacaccatct caatattatg gcgcgcgagc tggatgtcgc ggattaacgc ctgcatcaca
     7501 tcatctgact cggtcatcag ttgtagctga ttccctttga ccccagcgat cccctgacga
     7561 cgctcgcaaa gcttgaataa tggcgcagcg acactgctat tttcttcggc gaagatatgc
     7621 ttacaggctt taaggtcgtt aagccatttt gcggtggaag gccacatcgc tctggcgcgc
     7681 tcagcgcggc gtttgcctaa atggagctcg ccaacggcaa gataggcaat aattccgact
     7741 aacggcagaa tgtaaataat caacagccag gccatcgcgg agggaactgc gcgtcgtttc
     7801 attagaatgc gtaaagttac gcctgcaatg agcaaccagt atcccagaat ggccaaccaa
     7861 ctcaccaacg tataaacggt tgtcatagat tagaaatcct tttgaaagcg cattgttatg
     7921 agtttacgca tatggattca tctggcaaat aaaaacgcgg gaaaagcact ggtcagcgtc
     7981 gggggcgggg gtataatgac cattctgtta ttgcatagag tagttaacat gaagcggagt
     8041 agaacggaag tggggcgctg gcgcatgcag cgtcaggcta gccgacgtaa atcgcgttgg
     8101 cttgaggggc aatcgcgccg aaatatgcgt atccacagca tcaggaagtg cattctaaac
     8161 aaacagcgta actcgttatt gtttgcgatc tacaatatct aaatgtactg ggcaccattg
     8221 cggtgcccaa taagtttact cctccgtctt taaactctat ttttgcgccg attctttact
     8281 gtcagctttc gtttcttgtt catcgttatc gctgaaaata ccatgacctg agttcaacaa
     8341 cattgaaacg agcatatcat tattaatctc ttcaccattt aaaactcgcg ccagaatttc
     8401 gctgccaaac agttgcggtg agagaatgat atccggatgt accatcttaa tcttattcag
     8461 gtttttgcta tcactgacgg cgagaactgt tttgacatca ctgctcatat cttttgccga
     8521 gagtacaaca aacgcgttat ctgcatcgtt atcactcagc gccagaatgg cccggcaacg
     8581 atcgattccc gctttcttta atacagaact gtcattactg tcaccgggga taacatcagc
     8641 attatcgcct aagcgttgct caagttgctt gatatcatct tcaggcaagt tgctgataac
     8701 cgttacgttt tgtccgcgtt gattcagttg caggatcgta ttgatggcga gaatcgaatg
     8761 tccgcaaaca ataaaatgat ctttacgatg cattgtatga ttgtttcctt ttacaagttt
     8821 gttgaatccc ccgcggataa gcggaccaaa aattgaggtc atagatgtgg caaaaacggt
     8881 aatgccggaa ataatgaccg aaatagtgaa caatcgtgct gattcagaaa cagggacaat
     8941 atcgccatag ccgacggttg acatggtttc tatcgaaaaa tagaacgcgg tcatcaaact
     9001 ttctattcgc ggattaaaac cttcgcttaa ataaagcgca ccgtaggttg agtaaaacag
     9061 taatgtcgtg aaactaataa atgcaaaaat tgtcccggct gcggcgctac tgtgggagaa
     9121 gtctttgcgc agtatcagca aaaacaccag cgtaaaaatg caaaatccaa tactaaattt
     9181 cagccaggga taaaaatgca gggtgtaaat tagcgctatc aacaacaaaa tgatactgat
     9241 tgcccatgcg agctttgccc ggaacagtaa gccaatggag ttcaggacaa gaaagacgcc
     9301 gagcataaag agtggggcgt ttgccagcaa ggagagatcc aactctgaaa atgctttgat
     9361 atggaaaata tcaagcaaat tgaccgacat accatagatt gttttaaaaa taagcaatcc
     9421 atttaaaaag acggccagcg cgagaatgtc gtgccgaagg gtcacccata aatttgttgc
     9481 tgtttgtttg aatgtagccc agtgactcac tgatacctct gtaaccacaa ttttgtttaa
     9541 ttatcttagc gtgcacattt tctgaacata catgcagcgc gtgaatgtgt taaacaatag
     9601 tagatgactt tatccgcagt aatcaaagga tttgagcgag caactgtacc tcaggaaagg
     9661 tcatttttcc tgaatatgct cacatcatat aaagaaatac agataaagtt attatctgct
     9721 tgtggtggtg aatgcactga ccggctataa ggaaaggcca aacaagaaca cggttgcaaa
     9781 aaccgtgccc ttaaatattg aatctctatt cagaacactt tcttaaatgg tttcactgaa
     9841 acgtgttcat agactcctgc cgctacgtac gggtcagcat cggcccaggc ctgagctgct
     9901 tccagcgatt caaattcagc aataacggtt gagccagtaa atcccgcagc ccctggatcg
     9961 ttactgtcta ccgctggcat tggaccagct gtcaacaaac gaccttcatc atgcagtaac
    10021 tgtaaacgtg ctaaatgtgc cggacgaacg gaaaggcgtt tttcgaggct atcagctttg
    10081 tcttgagcgt aaataacata caacacgggc acaactcctt gttcgggaaa gttgtaaagt
    10141 acgttatttg aaagggcgaa gatctgcaac ggaaagatga tgtctttgtt aaggccatgc
    10201 ataaagtaag ggtaattacg ccaaaaatga cattttcact gatcctgatc gtcttgcctt
    10261 attgaatatg attgctattt gcatttaaaa tcgagacctg gtttttctac tgaaatgatt
    10321 atgacttcaa tgacccttga tttacctcgc cgcttcccct ggccgacgtt actttcggtc
    10381 tgcattcatg gtgctgttgt ggcgggtctg ctctatacct cggtacatca ggttattgaa
    10441 ctacctgcgc ctgcgcagcc gatttctgtc acgatggtta cgcctgctga tctcgaaccg
    10501 ccacaagccg ttcagccgcc accggagccg gtggtagagc cagaaccgga acctgagccg
    10561 atccccgaac cgccaaaaga agcaccggtg gtcattgaaa agccgaagcc gaaacctaag
    10621 ccaaaaccga agccggtgaa aaaggtacag gagcagccaa aacgcgatgt caaacccgta
    10681 gagtcgcgtc cggcatcacc gtttgaaaat acggcaccgg cacgcccgac atcaagtaca
    10741 gcaacggctg caaccagcaa gccggttacc agtgtggctt caggaccacg cgcattaagc
    10801 cgtaatcagc cgcagtatcc ggcacgagca caggcattgc gcattgaagg gcaggttaaa
    10861 gttaaatttg acgtcacgcc ggatggtcgc gtggataacg tacaaatcct ctcagccaag
    10921 cctgcgaaca tgtttgagcg tgaggtgaaa aatgcgatgc gcagatggcg ttatgagccg
    10981 ggtaagccag gcagtgggat tgtggtgaat atcctgttta aaattaacgg caccaccgaa
    11041 attcagtaag cataaagtca aaagcctccg accggaggct tttgactatt actcaacagg
    11101 taaggcgcga ggttttcctt caggatcaac cgcgacatac ttaaataatg cttctgtcgc
    11161 tttataccgt tgcccgattg gttcagacgc tacttttttc acccacactt caatattaat
    11221 gctgaccgat gtcgtccctt tctggacaca gcgtgcatag cagcacacca catcgccgac
    11281 cgcaaccggc cgtaagaaag tcattccttc aacccgcaca gtcactacgc gaccgtgggc
    11341 aatttctttt gccagaatag cgccgccaat atccatttgt gacattaacc aaccaccaaa
    11401 gatgtcacca ttggcattgg tatcggcggg catggctaaa gtacgtaaaa caagatcgcc
    11461 ctgagggacg ttatgtgttg tagacatggt aaaaccgact taaaaggaaa atcacaggcg
    11521 cgatgctact atgattttcc tgaggagaac agaggagata cagcgccagc cccgaaggac
    11581 tggcagtctg gttaggattt atcttcctgc ggcatgtggc ggtagatata gataccgctt
    11641 aacaatgtga agattaaggt aagggcggtc aggccaaaga ctttaaagtt gacccaaata
    11701 ttttgcggca gccagaatgc gatgtagatg tttgccagac cgcaaaggat aaagaaaaca
    11761 gcccaggcca gattcagctt cgaccatacc ggttgcggca gcgtgagttc tttacccagc
    11821 atccgctgaa ttagcggctt tttcatcacc cattggctga ctaacagggc tcccgcaaat
    11881 agagcataaa tgactgtaac cttccattta ataaactcat cattgtggaa gaacagcgtc
    11941 aagccaccga agacgaccac cagaacaaaa gtgatcaggg ccatcttctc aaccttacga
    12001 aagcgaaccc agctatagat aagcacaatc gccgtggcga cgatcagcgc cgcagtagct
    12061 gcatagatgt cataaatctt gtaaaacgcg aaaaagacaa ccagcggtaa aaaatcaaga
    12121 aactgcttca ttttacgatt ccgtaatcaa atgcttaagg ttattggcga atcaacatat
    12181 acaggcggaa cagatagatg agcaatatgg ctgaaatcag gttgctcaag gtgttcgcca
    12241 gtacggcacc aatttccggg gttaatgcgg caaaagaaga ggcaaaaagc agcaacagtg
    12301 tttttgccag caaccagctc agtactgcgg gtgccaccag acgcatattc gcccaagtca
    12361 gccgcatact gctacgcatc gaggcaaaaa tgcccatttt gtcctgtacc agcatcaccg
    12421 gtgccagggc cagcaaaatg gccatgataa ttcccggaac gaccaccagc atgatgccaa
    12481 tctgtactaa aagggtagtc agaaaaatca gaataaataa cttgggcaat atcggcgcac
    12541 tggcaccaat agctcgcagc gcactgactc tctgacccgc agacaccagc tggataatta
    12601 atattacgcc tccggcgaga atggcgttac cgatcaatcc tgaaaaagtg gaagccgctg
    12661 aagcttgcag caaaatttgt tgctgttccg gtgacatatt ctgaaccagg tcgaacaacc
    12721 cactactgcc gctaacgggt acgccgtcat tgagctgcgc aagctgtgca tcactgggtg
    12781 agaaaacatg ccctaacacc actgtgataa acgcacatag caacgatacc aacagaatgg
    12841 tcataaattg attacggaag aaatttccgg tgtcacggta tacggactgc gccgtgatag
    12901 acatgcactc tccttgagtt ttatgcaggt gttaattagc gggcaattgt accctggtta
    12961 agcctcgggt ggcagcatca ggtcttgtat ggaaaagcat atctttgtaa agcggaggta
    13021 attgctggcc ttcaggcatg ggcaaatcgc ggcaagtggc gaccgagtta atatttgcgt
    13081 agcgaaaata ttt
//
LOCUS       DAPHBJ010000123        13041 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.124, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000123 DAPHBJ010000000
VERSION     DAPHBJ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.124"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            398..1087
                     /gene="mutH"
                     /locus_tag="RHZ76_003100"
     CDS             398..1087
                     /gene="mutH"
                     /locus_tag="RHZ76_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HDV2813696.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALVGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            1156..1869
                     /locus_tag="RHZ76_003101"
     CDS             1156..1869
                     /locus_tag="RHZ76_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDV2813697.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            2007..2225
                     /gene="ygdR"
                     /locus_tag="RHZ76_003102"
     CDS             2007..2225
                     /gene="ygdR"
                     /locus_tag="RHZ76_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HDV2813698.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            2333..3373
                     /locus_tag="RHZ76_003103"
     CDS             2333..3373
                     /locus_tag="RHZ76_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HDV2813699.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(3405..4598)
                     /gene="lplT"
                     /locus_tag="RHZ76_003104"
     CDS             complement(3405..4598)
                     /gene="lplT"
                     /locus_tag="RHZ76_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HDV2813700.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(4591..6750)
                     /gene="aas"
                     /locus_tag="RHZ76_003105"
     CDS             complement(4591..6750)
                     /gene="aas"
                     /locus_tag="RHZ76_003105"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HDV2813701.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            7336..8367
                     /gene="galR"
                     /locus_tag="RHZ76_003106"
     CDS             7336..8367
                     /gene="galR"
                     /locus_tag="RHZ76_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HDV2813702.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(8374..9636)
                     /gene="lysA"
                     /locus_tag="RHZ76_003107"
     CDS             complement(8374..9636)
                     /gene="lysA"
                     /locus_tag="RHZ76_003107"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDV2813703.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            9758..10693
                     /gene="lysR"
                     /locus_tag="RHZ76_003108"
     CDS             9758..10693
                     /gene="lysR"
                     /locus_tag="RHZ76_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HDV2813704.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDNFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(10680..11372)
                     /gene="ygeA"
                     /locus_tag="RHZ76_003109"
     CDS             complement(10680..11372)
                     /gene="ygeA"
                     /locus_tag="RHZ76_003109"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDV2813705.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(11501..12919)
                     /gene="araE"
                     /locus_tag="RHZ76_003110"
     CDS             complement(11501..12919)
                     /gene="araE"
                     /locus_tag="RHZ76_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HDV2813706.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGI"
BASE COUNT         3308 a         3506 c         3205 g         3022 t
ORIGIN      
        1 ttgagtaatg aaaaagagta attcgtgacc caggtcacac ctctcattta cgggttgacg
       61 aaagatcaac caatgccgta tttatccaca gaatgtgcca ctaagttaag cactgaacca
      121 ctaaaaactg gagtttcgtc gcacgtcaag gctgtaaatg gaaacagtag tggaggtttt
      181 tcacagttat cccagctttc tgtggataac atggtgtaag atcctgttta ttttcagtga
      241 ccagatttgg aaaacccgtt gcagtgttgc gcaactcgat taccggcaac ctaaaaagca
      301 atataaatca gagaattgaa caacgcatgt ggaaaaagtt acactgcgaa tattcggcac
      361 ataattgctg tttgtttttt aaccaaggta tcatgacatg tcccaacctc gcccactgct
      421 ctctcctccc gaaactgaag aacagttgtt agcgcaagca cagcaacttt ctggttatac
      481 attgggagaa ctggcggcac ttgtcgggct ggttacgcca gagaatttaa aacgcgataa
      541 aggctggatt ggcgtgttac tggagatctg gctaggtgcc agcgcaggga gtaaacctga
      601 gcaagatttt gctgctctgg gcgtggaact taaaactatc cctgtggata gtcttggtcg
      661 tccgctggaa acaacattcg tttgtgttgc cccgttaacg ggcaatagcg gggtgacctg
      721 ggaaaccagc cacgtgcgcc acaaactcaa acgcgtactg tggataccgg ttgaaggcga
      781 gcgcagcatc ccgctggcgc agcgtcgcgt tggatcaccg ttgctgtgga gcccgaatga
      841 agaggaagac cggcagctac gcgaagactg ggaagaatta atggatatga ttgttctcgg
      901 tcaggttgag cggattaccg ctcgtcacgg ggagtattta cagatacgac cgaaagcagc
      961 gaatgcgaaa gcgcttaccg aagccattgg tgcccggggc gaacggattc tgacgctgcc
     1021 acgcggcttt tatttgaaga agaatttcac cagtgcgcta ctggcccgtc attttctgat
     1081 ccagtagcca tcgctttgac ctgccgcttt ccgggcatat aattaccgct tcattttttt
     1141 ggcagggctt tttagatgtt atttgcatgg ataaccgatc ctaacgcctg gcttgcgctc
     1201 ggtacgctga cgctgctgga gatcgttctt gggatcgaca atattatttt cctttctctg
     1261 gtggtggcaa agcttcccac agcacaacgt gctcatgcgc gccgtctggg gttggcggga
     1321 gccatggtta tgcgtctggc gctgctggca tcaatcgcct gggttacgcg cctgacgaat
     1381 ccgcttttta caatattcag tcaggaaatt tccgcccgtg atttgattct gcttctgggt
     1441 ggcttgttcc ttatctggaa agccagcaag gaaatccacg aatccattga aggtgaagaa
     1501 gaagggctga aaacacgcgt ttcatcattc ctcggcgcta tcgtgcagat tatgctgctg
     1561 gatattatct tcagcctcga ctcggtgatt accgctgtgg gtctgtcaga tcacctgttt
     1621 attatgatgg ccgccgtggt gattgccgta ggcgtgatga tgttcgccgc gcgctcgatt
     1681 ggtgattttg tcgaacgcca tccttcggta aaaatgctgg cgctctcttt cctgattctg
     1741 gtcggcttta ccctgattct ggaaagtttc gacatccacg taccgaaagg ttacatctac
     1801 ttcgcgatgt tcttctctat tgcggttgaa agcctcaacc tgattcgcaa caaaaagaac
     1861 ccgctctgat actccgtacg ctctcctgcg ggagggcgta ctcactcctg ttaccctcac
     1921 agtttaagat tttcctgctt tcaagatata taacgtcggt ttataaacag actattatca
     1981 taggtgagcc aaatgaggat aaccggatga aaaaatgggc agtaataatt tctgcagtcg
     2041 gactggcgtt tgctgtttcc gggtgttcca gtgattacgt catggcgacc aaagatggcc
     2101 gtatgatttt gaccgatgga aaacctgaaa ttgatgatga taccgggctg gtgagttatc
     2161 acgatcagca aggtaacgcg atgcaaatta accgtgatga tgtttcgcaa attattgaac
     2221 gttaacaaat aaggtcagca tccggctggc cttaagattt ttctctttcc cttttccctt
     2281 ccctctgcca tttttatatt ccttatgtcg tgattataaa aaggaaacgg ctatgcaata
     2341 tcaccgtata ccccacagtt cgctggaagt cagcacgctg gggcttggca cgatgacgtt
     2401 tggtgaacag aacagcgaag ccgacgccca cgcacaactc gactatgccg ttgctcaggg
     2461 cattaacctt atcgacgttg ccgaaatgta cccagtacct ccgcgccccg aaacgcaagg
     2521 gttaaccgaa acctacgtcg gcaactggct ggcgaaacat ggcagccgcg aaaagttaat
     2581 tatcgcctcc aaagtgagcg gaccgtcgcg caataatgac aagggcatcc gcccggatca
     2641 ggcgctggat cggaagaata tccgcgaagc gctgcatgac agcctcaagc gcctacagac
     2701 tgattacctc gatctttatc aggtgcactg gccgcagcgc ccaaccaact gctttggcaa
     2761 actcggttat agctggacgg attctgcgcc tgcagtttcg ctgctggata cgctggacgc
     2821 actggcagag taccaacgcg cgggaaaaat tcgttatatc ggcgtgtcga acgaaactgc
     2881 atttggcgta atgcgctacc tgcatctggc ggacaaacac gatctgccgc gtattgtcac
     2941 cattcagaac ccctacagtc tgttaaaccg cagttttgaa gtaggtctgg cagaagtcag
     3001 ccagtatgaa ggggtcgaac tgctggccta ttcgtgcctg ggtttcggta cgctgaccgg
     3061 gaaatatctc aatggtgcaa aacccgctgg cgcacgtaat acgctcttta gtcggttcac
     3121 ccgctatagc ggtgagcaaa cgcaaaaagc cgtcgcggcg tatgttgata ttgccagacg
     3181 tcatggcctg gaccctgctc aaatggcact cgcatttgta cgccgtcaac cgtttgttgc
     3241 cagcactctg ctgggcgcaa ccacgatgga gcagttgaaa actaacgtcg aaagtttgca
     3301 tctggagtta agcgaagacg tattagctga aattgaagct gtgcatcagg tttatactta
     3361 tccggcacca taagattaac gccggttaaa accggcgtta aatattaatg acggcgctgc
     3421 cagatccaca gcgccgttat tgccagcgca aacagcgcac cgaagccaat gccaatgggc
     3481 acgaccggga tgcctaccat taccgccagc gagtaaatgc ccagcatcaa caacatggcg
     3541 ctgttttcgc caaggttttg tactgcaatc gcattccccg ccccgacgct ttttttaccc
     3601 cgctcctgta gcaacgcatt gagcggaacg acaaaaaagc cccccatcac gccaatcagc
     3661 atcaacaagg cataggctgg cagcagctcg tgttgcaggg aaaaaatcag taccaccacg
     3721 ccaatcaaaa tcccggctgg catacagcgt gacacggttt ccagcgtaac taacttcgct
     3781 gccgcacctg cgccaaccac gataccaatc gctaccatcg cgttgagata ggtgggcgta
     3841 gcgttatcgg taatgcccag cgccaccggt acccacagca ccaacaggaa acgcagcgtg
     3901 acacccgctc cccagaataa actggtgccc accagcgaaa aacgcgtttc accattgcgc
     3961 catagcgagg tgcaggcatt caggaaactg cgggtcatgt tgatgagatt ccaggactgc
     4021 cccggacgcg ccgccgccag tttgggaatg tagatattgg caacgaccgc accaccgtag
     4081 gccagtgcgc atgcggccag ggcgacgagg acatgccagt cagccagcac accaccggct
     4141 acggaaccga gcaaaatcgc cgctatggta gaagcttcca ttaaaccgtt agctttcact
     4201 aacttactac ccgtggttaa ttcgccgaga ataccgtatt tcgccggtga ataggctgca
     4261 gcaccaacac ccaccagcgt atagccgaga aacggattga taccaaagca gatactggct
     4321 gcgcccagca gcttcaggcc gttggcaaac atcatcaccc ggcctttggc gaagctatcc
     4381 gccacctgcc cgacaaacgg cgcaaaaaga atgtaagcac ctacaaacac catttgcagg
     4441 atgggctggc tccactccgg atagaactgc gctttcagta acgccagagt ggcaaacagt
     4501 agggcattat cgccaaacgc agagagaaac tgcgccacga taaccgcttt catccccttc
     4561 gaccacaacg aagtgttagt gtgcactgac tcactcatcg tgttgttccg cttcgtctac
     4621 ccagcttttc aacgtgacaa agtcaggttt gccgctgcca agtaatggca tctgtttcag
     4681 atagcgaata tcgcgcggta cagcaagctc cggcacgccg tgctcgcggg catactgttg
     4741 caacttatcg cgcgtcagtt cgttatctgt ggtgaaaagc accagtgcct cgcctttgct
     4801 ggcatcgctc ttaatcgcag tggcatggac tttatctggc gaaacaccaa gtgccagttg
     4861 ttccaccatt tccagcgaca ccatttcgcc tgcaattttg gcaaagcgtt ttgcgcggcc
     4921 ctgaatctgc acaaagccct gctcgtcaaa acgcacaata tcgccagtgt cataccagcc
     4981 gcgctccatt tcgccgcgaa cattctcggc ggtgggcact tccagtacac ctggcttctc
     5041 cacccgcaga tagccgttca ttatgttcgg ccctttcagt tgcaggcgtc cgccctcttc
     5101 gataccagga accgacaaca ggcgcgcatc cattcctggt agaatacgcc ctaccgtacc
     5161 gggtttcgcc gccatcggta cgttgataga aacgacaggc gcgcactcgg ttacaccata
     5221 gccttcaagg atgcgcaggc caaatttatc ctgccaaagc tgtttggtac tttcttgtag
     5281 tttttctgcg cctgccacca catagcgtag acgatagaag tcatacgggt tggcgaaacg
     5341 cgcgtagtga ccgaggaaag tcgaggtacc gaacaacacg gtgcaactgc ggtcatacac
     5401 caactccggc acaatgcggt aatgcagcgg gcttggataa aggaacactt ctgcacctgt
     5461 aagcagtggc gtaaacaggc ctaccgtcag cccaaaggag tgaaacagcg gtaatgccga
     5521 cataaagcga tcgttggtgg tgaagtcggc aatcgtttta atctgctcga cgttcgccag
     5581 aatgctttta tggctatgga cgacgccttt tggatggcct tcagaaccgg aggtaaaaag
     5641 gatcagcgcc tcttcttccg gctgctgttt aacctgtgcc agacgcggca tcagcaaatg
     5701 agcgaagatc catactttgt cggcagtggt gacatctgct tttaaatctt ccagatagac
     5761 ccagcgcacc tgagtaagtt gctccggcag atgccagagt ttgcctttat cgagaaactg
     5821 gcgggaagtg aagatggttt tgatttcagc cgccgtaata gcactggtca gccctttcac
     5881 cccggcagtg tagttcatca ttgcgggaat gcggcgacgg gcgatggccc caaaaatcac
     5941 tgccgcactg atgcctgcat tgggcagcat taagccgatg cgttcgcctt caacactgta
     6001 tttttcaagg atgcgtccaa caaacagcgt tttcgtaagc aatttgcgat aggagtctgg
     6061 ggtaaagttg acgtcttcga cacatttctt cccggctccg aagcggtaca ttgcactcag
     6121 taaagattcg tacagcgttt cacgcgggcg caccgccatt cgcgcttcca tcattatttg
     6181 atgcagcatt tcgccagcga ttttgcgacg gtcacgggca cgcggcgcat ccggcatcgc
     6241 cacctgcgtt ggtggcaaaa tatgcagagt aatttgcggg aacaagcgac gtttaaccag
     6301 acctttcagg cggctgaagt gcgtaagttc cgccccttca atacgcacag gaataaccgt
     6361 tgcaccagac ttcgccgcga caaaacccgc gccatcgtag attttcatca gcgagcctgt
     6421 agtggtgatg cgtccttcag ggaaaatcac cactggtcgg ccctgttcca ccagacgtac
     6481 cagatgttta atagccatag gttgcgtcgg gtcgagagga acaaagtcga taaatgattt
     6541 cagccaacgc atataccact gttggcttat tgaggtgtaa acggcaaaca ctggacgcac
     6601 aggtaaaaac agtccaagca aaatgccatc aataaaagag acgtgattag gcgtaattag
     6661 aacgcgctcg cccttcagtg cctgggtgtc acccgtaacg cgaacgcgat acaaaacacg
     6721 gcacaaattt cgaaaaaagc taaaaagcat tccaactccc tttgctctga ttcagtaaaa
     6781 gcgaatggag ggagattaca cgagataaag aacgcgagcg acagtaaatt aggtgcgaaa
     6841 aaaaacctgc gcatccgcgc aggttggtgc aagagacagg gtacgaagag cgtaccgaat
     6901 aatctcacca atcaatacct ctgggatctt gattgtggtc tgcacgacga ctcttcgcca
     6961 gcgagaaaac gcaaaggaat gaagggaaat gcaacgaggt gtgtaaattg tcggttactg
     7021 ttacagattg atgaccggcc aaaaaaaacc tgcgcatctg cgcaggctgg tgtaattcat
     7081 gtgctcaacc cgaagttgac ttcacctatc aatacctctg ggatcaccac tttagcaacc
     7141 tgaagccaaa cgccaccagc ggtcaatcgc aacagcgttt cgcaaagtgt aaccaaaggt
     7201 ttgcaatctc tttttttgtc ttacttattt gcgtttggct cacattccca cgatgaaaac
     7261 acgccacccc ttgaaccaac gggcgttttc cgtaacactg aaagaatgta agcgtttacc
     7321 cactaaggta ttttcatggc gaccataaag gatgtagccc gactggcagg cgtttcagtc
     7381 gccaccgttt cccgcgtcat taataattca cctaaagcca gtgaagcttc ccggctggct
     7441 gtgcatagtg caatggagtc tcttagctat cacccgaacg ccaacgcccg tgcgctggcg
     7501 cagcagacca ctgaaacggt cggtctgatc gttggtgatg tttccgatcc gtttttcggt
     7561 gcaatggtga aagcggtcga acaggtggct tatcacaccg gtaatttctt attgattggc
     7621 aacggttacc acaacgaaca aaaagagcgt caggctattg agcaactgat ccgccatcgc
     7681 tgtgctgcgt tggtcgtcca tgccaaaatg atcccggatg ccgatttagc ctcattaatg
     7741 aaacaaatgc ccggtatggt gctgatcaac cgtatcctgc ctggctttga aaaccgttgt
     7801 attgctcttg acgatcgtta cggtgcctgg ctggcaacgc gtcatttaat tcagcaaggt
     7861 catacccgca ttggttatct gtgctctaac cactctattt ctgacgccga agatcgtctg
     7921 caagggtatt acgatgccct tgctgaaagt ggtattgcgg ccaatgaccg gctggtgaca
     7981 tttggcgaac cagacgaaag cggcggcgaa caggcaatga ccgagctttt gggacgagga
     8041 agaaatttca ctgcggtagc ctgttataac gattcaatgg cggcgggtgc gatgggcgtt
     8101 ctcaatgata atggtattga tgtaccgggt gagatttcgt taattggctt tgatgatgtg
     8161 ctggtgtcac gctatgtgcg tccgcgcctg accaccgtgc gttacccaat cgtgacgatg
     8221 gcgacccagg ctgccgaact ggctttggcg ctggcggata atcgccctct cccggaaatc
     8281 actaatgtct ttagtccgac gctggtacgt cgtcattcag tgtcaactcc gtcgctggag
     8341 gcaagtcatc atgcaaccag cgactaaccg cagttaaagc aattccagcg ccagtaattc
     8401 ttcgatggtc tggcgacggc gaatcaaccg cgcctgacca ttatcaaaca gaacttctgg
     8461 taacagcgga cggctattgt agttggatga cattgatgcg ccatatgccc ctgtatcatg
     8521 cagtaccaga taatcacctg ccttcacttc cggcaaggcg cgggtttcaa catttccccc
     8581 ttcctgctgg gtaaagacat cgcccgattc acataacggt ccggcgacga cggtttccac
     8641 cgttggcgcg tgttccagag aacgaccatc agctgccagg gcactgatat ggtggtaact
     8701 accgtacatt gccgggcgca tcagatcgtt gaacccggca tcaaccagca caaagtggcg
     8761 gctccccatt tgtttgacgc tccgcacctg agtaattaat acgccagact gcgctaccag
     8821 gaagcgaccc ggttcaattt ccagtttcac agggtggccc aaatggcggg cgatttgctc
     8881 acgcgcggca ttccacagac cataataatg ttcggtatca accgcctctt caccctgttg
     8941 ataaggaaca gaaagcccac cgcccgcaga aatagcctgt aaatcctgac cgaattcgat
     9001 gacctgacgc accatagcac cacacacctg ttccagatgg gcataatcaa cgccagaacc
     9061 aatgtgcatg tgaatgccga ccagctgcag atgatgacgt tgtatcacgt ccagtgcggc
     9121 gggcagatcg gtgtaccaga taccgtgctt gctgttttcg ccaccggtat tggttttttg
     9181 gctatgtccg tgaccaaacc ccggattaac gcgcagccat acccgatgcc ctggcgaaac
     9241 ctggcccagt tggtcgagca tatcaacaga acccgcattc accggaattt gcaattcact
     9301 gacgcgttca agcgtcgcct gatcgataac atctgccgta aaaacaatat catcggggtg
     9361 cgtttgcgga ttgtaacccg ccgccaacgc acgctctatt tcgcctaacg agacggaatc
     9421 cactttcacg ccctgctcac gcattaagcg caaaatatga atattggaac aggctttctg
     9481 tgcaaagcgc accacatcaa actgtttcag cgctgcaatc tgccgacgaa taatttgcgc
     9541 atcgtagacc cacaccgggc agccaaattc agcgggcaaa cgcagcagat tttcggcggt
     9601 gagatcggta tcggtgctga acagtgaatg tggcataaca aactccagat aagtgctttt
     9661 ttatgattac gccacatcat aaaaagaata aaaaatatcg atttatgtcg agtctatgca
     9721 aaaatgatat ggattaccgg attgcgagag agcgctaatg gccgccgtta acttacgtca
     9781 tattgaaatt tttcatgcgg taatgaccgc cggaagcctg actgaggcgg cacacctgct
     9841 acacacctca cagccaaccg tcagccgcga acttgcgcgc tttgagaagg tgatcgggct
     9901 gaaattgttt gagcgcgtac gtgggcgatt acatcctacc gtgcaaggac tgcgtctgtt
     9961 tgaagaagtg caacgatcct ggtacggact ggatcgcatt gtcagcgccg cagaaagtct
    10021 gcgcgagttt cgccagggag aactgtctat tgcctgcctg ccggtctttt cgcaatcttt
    10081 tttaccgcag ctcctgcaac cctttctggc acgttatccc gatgtcagct taaatatcgt
    10141 gccccaggaa tcaccgctac ttgaagagtg gctctcggcc cagcgtcatg atttaggact
    10201 cactgaaacg ctccatacgc ctgcgggaac agaacgtacc gaattactct ctttagatga
    10261 agtgtgtgtg ttacctccgg gtcatccgct ggcggtaaaa aaggtattaa cgccggataa
    10321 ttttcagggt gagaactaca tcagcctttc ccgtactgac agctatcgcc agttgctgga
    10381 tcagctattt actgaacatc aggttaaacg acgcatgatc gtagaaaccc acagcgccgc
    10441 gtcagtctgc gcaatggtac gggcgggggt aggtgtttcg gtggttaacc cgctcaccgc
    10501 actagattat gcggcaagcg gtttagtggt gcggcggttc agtattgcgg ttccgttcac
    10561 cgtcagcctg atccgccccc tgcaccgccc gtcatcagcg ctggttcagg cgtttagtgg
    10621 gcatttacaa gcggggttac cgaaactggt cacttctctt gacgctattt tgtcgtcagc
    10681 tacgacagca taaaagcgac agcatcctcg gcatggatcg ccgcggtatc aaacacaggc
    10741 aaaacactgc gctcttctgg caccagtaaa ccaatttctg tgcagccaaa aatgacgccc
    10801 tgtgcgccct gttctgcaag gcgagcaatc acttgcgcat aataagcgcg tgacgcttcg
    10861 gtaaattgcc ccagacacag ttcttcaaaa ataatctgat taattttcgc ccgttcatcc
    10921 gcttcaggaa taagacaatt gatggaaaat tgttccgtca gccgcccgcg ataaaaatcc
    10981 tgttccatgg tgtaacgcgt acccagcagc gccacacgag tcattccggc cccggtaatt
    11041 gcacgtccgg tggcatccgc aatgtgtaag aaaggcagag tgcaacgtga ctcaatggca
    11101 tccgccactt tatgcatcgt attggtacat aacacaatac cttctgcgcc cgcccgctgt
    11161 aagccaagcg ccgcctcagc cagaatgtcc ccggttttat cccattcccc gcgacgctgg
    11221 cactcttcta tttcatgaaa atcgacgcta tgtagcagca cttgcgcaga gtgaagccca
    11281 ccaagccgct gtttaatgcc ttcatttatc aaacgatagt aaggaatggt ggattcccag
    11341 ctcattcctc ccagcaaacc aattgttttc attcctctct cctgatgtgc gttaccgcaa
    11401 caaggctgaa taaggaacga gacaaacgcc tcaacggcca agtgcccgat ctctattaac
    11461 gaaaaaaggg ccggatgtac agcacatccg gcccgtgaaa tcagatgccg atatttctca
    11521 acttctcgcc tgccatcagt ttgcgttcga tatgttccag cgtgacattt ttggtttccg
    11581 gaatgagcca gaaagtgatg cccacaaacg caatgttcag cgcagtgtag agccagaacg
    11641 taccggcagc gccaatgctg tcgagcagcg tcaggaaggt cgcgccgata atcatattcg
    11701 acacccagtt cgtggtggtc gaacaggtaa taccgaaatc gcggcatttc agcggctgaa
    11761 tttcagagca caggatccac accactggcg cggcgctcat cgcataaccg gcaatacaca
    11821 tcatcgtcat gccaacagag agccaggaca agccactgga agccgtaccg ttatcaaact
    11881 gcatcaggca atagcccagc accagagtgc ctaacgccat cacgctgaaa ccaattttca
    11941 gagccggttt acgccctgct ttatctaccg taaacaccgc aataaaggtg gcgaacataa
    12001 aggtcagccc tacgaccaga gtcgcaatca tctgttgttc tgtggtcgta aagcccgcca
    12061 ttttgaagat acgcggcgcg tagtacatga tgatgttcat accggtaaac tgctgcatcg
    12121 cctgcaacaa cataccgaga aacacagcac gacggacgtt acggttgatc ttaaacagtg
    12181 cccaaccgcc ctgttttaac ttcaggcttt cacgaatttc gttgagttct tctcgcgctt
    12241 tttccgacgt atcgcgcagc atacgcaata cttcttccgc ctcaatatga cgcccctttt
    12301 ccgccagcca gcgcgggcta tttggcagga agactaccag aataatcagc agaactgctg
    12361 gtaaagcaag aacccccaac attgcgcgcc agttaccgct ataactgaac gctgtatcgg
    12421 ataaaaacgc cagcacgatg ccgagtgtga ccatcaactg gtacatactg atcatcttac
    12481 cgcgaacgtt ttcacttgcc atttcagaaa gatacagagg agcggtgtaa gacgcgatcc
    12541 cgacagcaat gcccagcacc acacgagcgg cgattaacat ctctacgctg gtcgcaaaag
    12601 cggaccctat agaaccgagt acaaacagga tggcccccgc catcaggctg tatttacgcc
    12661 ccaggcggaa cgacagccaa ccattaaaca gcgcaccaat tgctgcaccg agcatcatgc
    12721 tactaaccac ccattcctgc aaacgactgg tcagcacaaa gtgatcggta atgaacggca
    12781 acgctccggc gattacgccg atatcaagac caaataacaa tcctgcgacc gcagcagcta
    12841 ccgaaacaaa catattcata cgccgcgtat cccgcaaaga acgtggcgtt aaagcagatt
    12901 ccgtattgat agtaaccatt ttttcctgcc agcagagagt aagacatagt gaaaaaatac
    12961 gtgaacaact cacgcaggtg tcaggtcgga aacagcataa atatggatta aattgctgcg
    13021 acatgtcgtt atgtgatgga t
//
LOCUS       DAPHBJ010000124        13020 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.125, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000124 DAPHBJ010000000
VERSION     DAPHBJ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.125"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            67..1122
                     /gene="phoE"
                     /locus_tag="RHZ76_003111"
     CDS             67..1122
                     /gene="phoE"
                     /locus_tag="RHZ76_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HDV2813707.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1161..1562)
                     /gene="crl"
                     /locus_tag="RHZ76_003112"
     CDS             complement(1161..1562)
                     /gene="crl"
                     /locus_tag="RHZ76_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDV2813708.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1620..2864)
                     /gene="frsA"
                     /locus_tag="RHZ76_003113"
     CDS             complement(1620..2864)
                     /gene="frsA"
                     /locus_tag="RHZ76_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDV2813709.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(2956..3414)
                     /gene="gpt"
                     /locus_tag="RHZ76_003114"
     CDS             complement(2956..3414)
                     /gene="gpt"
                     /locus_tag="RHZ76_003114"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDV2813710.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3675..5132
                     /gene="pepD"
                     /locus_tag="RHZ76_003115"
     CDS             3675..5132
                     /gene="pepD"
                     /locus_tag="RHZ76_003115"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDV2813711.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQDILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIKSVGHYWTLLTELLKEIPAK"
     gene            complement(5189..5710)
                     /locus_tag="RHZ76_003116"
                     /pseudo
     CDS             complement(5189..5710)
                     /locus_tag="RHZ76_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5709..5912)
                     /locus_tag="RHZ76_003117"
                     /pseudo
     CDS             complement(5709..5912)
                     /locus_tag="RHZ76_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="mutations in this gene result in a compromised
                     ability for drug-inducible mexXY expression; expression is
                     inducible by the same ribosome-targeting agents that
                     induce mexXY; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            complement(6158..6610)
                     /locus_tag="RHZ76_003118"
     CDS             complement(6158..6610)
                     /locus_tag="RHZ76_003118"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV2813712.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMISSQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITCVERYIDMLFVDPEYTRRGVACALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQY"
     gene            complement(6620..7018)
                     /gene="yafO"
                     /locus_tag="RHZ76_003119"
     CDS             complement(6620..7018)
                     /gene="yafO"
                     /locus_tag="RHZ76_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HDV2813713.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKQARDNNQMHKIGKMAEAFRMRF"
     gene            complement(7021..7314)
                     /gene="yafN"
                     /locus_tag="RHZ76_003120"
     CDS             complement(7021..7314)
                     /gene="yafN"
                     /locus_tag="RHZ76_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HDV2813714.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEKYLNDMTDDDFNDFKE"
     gene            complement(7366..8421)
                     /gene="dinB"
                     /locus_tag="RHZ76_003121"
     CDS             complement(7366..8421)
                     /gene="dinB"
                     /locus_tag="RHZ76_003121"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDV2813715.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(8492..9277)
                     /gene="lafU"
                     /locus_tag="RHZ76_003122"
     CDS             complement(8492..9277)
                     /gene="lafU"
                     /locus_tag="RHZ76_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HDV2813716.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRAFSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            9249..10961
                     /locus_tag="RHZ76_003123"
                     /pseudo
     CDS             9249..10961
                     /locus_tag="RHZ76_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(11177..11674)
                     /gene="rayT"
                     /locus_tag="RHZ76_003124"
     CDS             complement(11177..11674)
                     /gene="rayT"
                     /locus_tag="RHZ76_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HDV2813717.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHT
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVERGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(11850..12623)
                     /locus_tag="RHZ76_003125"
     CDS             complement(11850..12623)
                     /locus_tag="RHZ76_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDV2813718.1"
                     /translation="MHDQPLLRMSLPSIPSFVLSGLLLLCLPFSSFASATTSHISFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALVENKHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            12809..>13020
                     /gene="dinJ"
                     /locus_tag="RHZ76_003126"
     CDS             12809..>13020
                     /gene="dinJ"
                     /locus_tag="RHZ76_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HDV2813719.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDV"
BASE COUNT         3251 a         3325 c         3266 g         3178 t
ORIGIN      
        1 actttcctgt tttcaacggg actctcccgc tgaatattcg cgcgttaatt aaaatcagga
       61 atgaaaatga aaaagagcac tctggcatta gtggtgatgg gcattgtggc atctgcatct
      121 gtacaggctg cagaaatata taataaagac ggtaataaac tggatgtcta tggcaaagtt
      181 aaagccatgc attatatgag tgataacgcc agtaaagatg gcgaccagag ttatatccgt
      241 tttggtttta aaggcgaaac acaaattaac gatcaactga ctggttatgg tcgttgggaa
      301 gcagagtttg ccggtaataa agcagagagt gatactgcac agcaaaaaac gcgtctcgct
      361 tttgccgggt tgaaatataa agatttgggt tctttcgatt atggtcgtaa cctgggcgcg
      421 ttgtatgacg tggaagcctg gaccgatatg ttcccggaat ttggtggcga ttcctcggcg
      481 cagaccgaca actttatgac caaacgcgcc agcggtctgg cgacgtatcg gaacaccgac
      541 ttcttcggcg ttatcgatgg cctgaactta accctgcaat atcaagggaa aaacgaaaac
      601 cgcgacgtta aaaagcaaaa cggcgatggc ttcggcacgt cattgacata tgactttggc
      661 ggcagcgatt tcgccattag tggggcctat accaactcag atcgcaccaa cgagcagaac
      721 ctgcaaagcc gtggcacagg caagcgtgca gaagcatggg caacaggtct gaaatacgat
      781 gccaataata tttatctggc aactttctat tctgaaacac gcaaaatgac gccaataact
      841 ggcggctttg ccaataagac acagaacttt gaagcggtcg ctcaatacca gtttgacttt
      901 ggtctgcgtc catcgctggg ttatgtctta tcgaaaggga aagatattga aggtatcggt
      961 gatgaagatc tggtcaatta tatcgacgtc ggtgctacgt attatttcaa caaaaatatg
     1021 tcagcctttg ttgattataa aatcaaccaa ctggatagcg ataacaaatt gaatattaat
     1081 aatgatgata ttgtcgcggt tggcatgacc tatcagtttt aatggatatt gccggatgtg
     1141 atacatccgg cacatttcac tcacgccgtt aacttcaccg gttcatcacg aaaatcatcc
     1201 gccggttcca gcttcagatt cagcgtcgtc agcagttcac gcagcttctc gtgaaactca
     1261 cgtagggtgt gctccagtcg ttcaaccact tcagtgtctt ttaccggaac actcttccag
     1321 tcgcctgctt tatcgaacag accaaactgg taactgtagg taaaacggga ttcctgcgct
     1381 tcaagctcca tccaccagcc ccagaactca cgcacttccg gtgccggttt cacgttgacg
     1441 catacagcca gacaatcgaa aaagaatcga ttatcttcgc acttaccttc acgaatatac
     1501 gggcctagtg cggtaaattt tttgatcaat ctgctcttcg ggtgtccact cggtaacgtc
     1561 attgcaatct cctgttgtga tgcaactgtt ttaccaaatt ggcaaatttt agcaaatttt
     1621 taacacaaac gtttttcgat ccagtctgtg atttcctgaa gacctttgtc aaaattccga
     1681 tacaccgggt taaatgggat ctctaataat ttaccgtcag cagatgatga ggtgattaag
     1741 cgtgagtcct cttccgggct gaacggatcg ttcttccagt agcccgataa cattggcgtt
     1801 gggcagcgac gtcccagcaa tccttgcact tttaatgaat agcgattcag ctccacgcgc
     1861 aacgcttcgt cggaagcatc atgcatcccc aaacgactcg ccagaacgtc aagatacatt
     1921 tccggcacct gttgctggca cttaaaatca ctcaacaggg tatgaactac cggaccaaga
     1981 caggcaaccg ctttcagacg cggcgattca aggtatgcca gacgcacggc aacgttagcg
     2041 ccgaaacgga aaccaaaggc cgcgacgcga gtgtgatcca cccacggtac gttaggcagc
     2101 gcctttaaga cgtgctgatg caacaggctg gagtcctggg tcagcttcca ttttgaagaa
     2161 aagcccaccg acggcatatc aatagtcagc atcgcaatgc cgcgcggcgc aaaataacgt
     2221 tcgtacaggc tgtaatagtc cgtctgcatc gcatccagac caccacacat taatactgtc
     2281 gggaacggac catcgccttt cggcatatgc aaaaagccgg tgatgggcgc accgccgggt
     2341 acggtaaact ccatctgccg catcgtgccc ggcagacgct gagcggcctc ttcataggcg
     2401 cggtttgaca aagcctgcgc ttgctcggcc aggtcatctc ctttcagatg aggataggca
     2461 gcaatgttat acaacgtagc cgcatgcagc cagtgacgac cactgagttg cggatcttct
     2521 tccgcacagg ctttttgttg ccacaccatc gcctgggtgg cccactcata aatccagttg
     2581 ccaccacggt agccaatcac agtatcgtat aaatcatcgt cggtacgttc agcatcgctc
     2641 atcacaatac gtgcctggac gtcgaggatt tcacgtgggt caataccgcg ccagatccac
     2701 atcagacggt taatcatgcg ataccagtga gggatggttt taccatcaag agccgactgc
     2761 acaggcggtt gtgcgccgtg attaaagcgg cggactagcg tcgaggtttc aggatgttta
     2821 aagcggggtt tgaacagggt ttcgctcagg tttgcctgtg tcatggatgc agcctccaga
     2881 atacttactg gaaactattg taacccgcct gaagttaaaa agaacaacgc ccggcagtgc
     2941 caggcgttga aaagattagc gaccggagat tggcgggacg aatacgacgc ccatatccca
     3001 cggctgttca atccaggtat cttgcgggat atcaacaaca tagtcatcaa ccagcggacg
     3061 accagccggt tttgcgaaga tggtgacaaa gtgcgctttt ggatacattt cacgaatcgc
     3121 aaccgcagta ccaccggtat ccaccaggtc atcaataacg atgaagcctt cgccatcgcc
     3181 ttctgcgcgt ttcagcactt taagctcgcg ctggttgtcg tgatcgtagc tggaaataca
     3241 aacggtatcg acatgacgaa tacccagttc acgcgccagt aacgcacccg gtaccagacc
     3301 gccacggctt acggcaataa tgcctttcca ttgttcagaa ggcatcagtc ggcttgcgag
     3361 tttacgtgca tggatctgca acatgtccca ggtgacgatg tatttttcgc tcatgtgaag
     3421 tgtcccagcc tgtttatcta cggcttaaaa agtgttcgag gggaaaatag gttgcgcgag
     3481 attatagaga tctggcgcac taaaaaccag tatttcacat gagtccgcgt ctttttacgc
     3541 actgcctctc cctgacgcgg gataaagtgg tattctcaaa catatctcgc aagcctgtct
     3601 tgtgttgaca acattttctg ctaaccctgt gacctgcaat actgttttgc gggtgatcga
     3661 caaggagact taacgtgtct gaactgtctc aattatctcc acagccgctg tgggatattt
     3721 ttgccaaaat ctgttctatt cctcacccgt cctatcatga agagcaactc gctgaataca
     3781 ttgttggttg ggcaaaagag aaaggtttcc atgtcgaacg cgatcaggta ggtaatatcc
     3841 tgattcgtaa acctgccacc gcaggtatgg aaaatcgtaa accggtcgtc ttgcaggccc
     3901 acctcgatat ggtgccgcag aaaaataacg acaccgtgca tgacttcacg aaagatccta
     3961 tccagcctta tattgatggc gaatgggtta aagcgcgcgg caccacgctg ggtgcagata
     4021 acggcattgg tatggcgtct gcgctggcgg ttctggctga cgaaaacgtg gttcacggcc
     4081 cgctggaagt gctgctgacc atgaccgaag aagccggtat ggacggtgcg ttcggcttac
     4141 agagcaactg gttgcaggct gatattctga ttaacaccga ctccgaagaa gaaggtgaaa
     4201 tctacatggg ttgcgcgggg ggtatcgact tcacctccaa cctgcatttg gatcgtgaag
     4261 cggttccagc tggctttgaa accttcaagt taaccttaaa aggtctaaaa ggcggtcact
     4321 ccggcggtga aatccacgtt ggcctgggta atgccaacaa actgctggtg cgcttcctgg
     4381 cgggtcatgc ggaagagctg gacctgcgcc ttatcgattt caacggtggc acactgcgta
     4441 acgccatccc gcgtgaagcc tttgcgacca ttgctgtcgc agctgataaa gtcgacgccc
     4501 tgaaatctct ggtgaatacc tatcaggaca tcctgaaaaa cgagctggca gagaaagaga
     4561 agaatctggc cttgttgctg gactctgtag cgaacgataa agctgctctg attgcgaaat
     4621 ctcgcgatac ctttattcgt ctgctgaacg ccaccccgaa cggtgtgatc cgcaattccg
     4681 acgtggcaaa aggtgtggtc gaaacctccc tgaacgtcgg tgtggtgacc atgactgaca
     4741 ataacgtaga aattcactgc ctgatccgtt cactgatcga cagcggtaaa gactacgtgg
     4801 tgagcatgct ggattcgctg ggtaaactgg ctggcgcgaa aaccgaagcg aaaggcgcat
     4861 atcctggctg gcagccggac gctaattctc cggtgatgca tctggtacgt gaaacctatc
     4921 agcgcctgtt caacaagacg ccgaacatcc agattatcca cgcgggcctg gaatgtggtc
     4981 tgtttaaaaa accgtatccg gaaatggaca tggtttctat cgggccaact atcaccggtc
     5041 cacactctcc ggatgagcaa gttcacatca aaagcgtagg tcattactgg acactgctga
     5101 ctgaactgct gaaagaaatt ccggcgaagt aattatttga tttgctgccg gatggcgttt
     5161 aatcgccttc cggcagtttc atccttcatt atccttcgat aaaagccatc cctgtaaatg
     5221 tccgtcgcgg gttgccacgt tcaatctggt gatggaacat tcgccgctgc gatttcagcg
     5281 ccgcgctatt ttcctgttgc tgctgctcca gcttccaggc aatcagcaat cgcgccagcc
     5341 gtttgttagc atgctggctg cgctctgact gaaccttcac gctaataccg gatgccagat
     5401 gcgtggcgcg taccgccgag tcggttttat tgacatgttg accgcccggc cccgacgaac
     5461 gcagcgtctc ataacggatt gcatccgatt gttccttctc atcagcggta aaacgcccaa
     5521 tgcccagaaa ccagtttttg cgcccatgat gaggccgata cggactcgga caaatccact
     5581 gaatagtgcc acaccagctt tcgcttaatg cccatgcgtt atcgccatcc agagaaacca
     5641 gcgccgaacg cagcgtgtca gagtagcggc ccgtttctgt ttccagcacc gttacagcga
     5701 cgtcttgtcg ggtaaaaacg accaacagag aagaacgctg cgccaatcgg gtagccgcgt
     5761 ccggggtgta aatctggcat ccccactacg cgctgcatgt tgggtaaatt tgccgtggtg
     5821 tgtaattgtt ggatcgctaa actctcgatc cacaggtcat cagatgcgat ggtaaatacc
     5881 gcatcggata agggacgaat atatttgccc atgtaccatt ccatttatac tcgtcatact
     5941 tcaagttgca tgtactgcga ctcgaattat ttagagtata taaatataaa aatcaggaaa
     6001 tggcagacga atccagggca agaaaaaaag aattaatcct ggaaaggtct gcaaagagtc
     6061 attgataaag gatttgtcat gatttacctc cttgcagttt gtggaataag aaaacgcgca
     6121 cataccaggc gggcgttatt ttcattgcaa gctggattta atattgcggt ttatatcgca
     6181 tataaaaatt agtaaaccac gttccccggc attcaacgcg ctgctgctta actgtctgaa
     6241 aaccataacg ttcaaaaaag ggttttgcgg ttatgctggc gtccaccgta agttcggatt
     6301 cagacttaat caaaggtttt aacaaagcgc aggcaacccc gcggcgggtg tattcagggt
     6361 caacaaataa catatcgata taacgttcaa cgcaggtaat aaaaccaacc ggttgtgcat
     6421 taatgacggc aacccgcact tgtgatttcg cgagtttctc cttccagcga gattcgtcaa
     6481 tctgcgccca ggcggcaatt tgttgtggtg aataatgctg actggagatc atcgtaaccg
     6541 ctctaatgaa aatagcgcat agttgctgaa aatcgccagg ctgatagtct cttatttgta
     6601 tgttattcat aatataaatt caaaaacgca tgcgaaacgc ttctgccatt ttcccaattt
     6661 tatgcatttg gttgttatct cgagcctgtt tatgaggttc aggtttcaga atggcaatga
     6721 gcaaccatgc ttgctcatca aacgcaccct gacaatatac caaatgcgct tcgtcattcg
     6781 ttctgctgaa ttggcgcaac tgtggcggaa atggattatt cacatttgcc agatgaatat
     6841 gagcaactcg ctcaaatttg attaatggcc aggtaaacga gtcgtcgtag agtgcatcgc
     6901 gaccaaatat atctggcaaa acaccgtcac gcttatagga aataaaatcc gccgttaacg
     6961 catcaagttc ctctgctgta agttgcaggc gaataagttt tgttttgaat acccgcatcc
     7021 ttattcctta aagtcattga aatcatcatc cgtcatatca ttaagatatt tttcagctcg
     7081 gcgtgtaatt tcctctaatc ttgcagcact tggacggaag cgcgccttta tgggcttttt
     7141 ctcctcttgt tcagcaagca tgtccattaa cgcttcgaat gcgctggcac ttaagagata
     7201 tcctgcgggg cgattattag aaagaaccgc aaccggttga tcaataaagt atttagctgg
     7261 gtttttacgt aactcagtaa tattgaccga tttttcagcg agaattcgat gcatgcagtg
     7321 atcccctcat aataatgcac accagaatag acataatagt atacatcata atcccagcac
     7381 cagttgtctt tccatttgcg gatcaagcaa cgtcacatgc agccccacca gtcgcacacc
     7441 gcgcccgccg cggcgttcat cccaggtttt acgcgcggtg gcgattaagt cagctttatt
     7501 cagccgcggc cagacgtgct cctgagtggt ttgctgaaaa tcgtcgaact ttaatttcac
     7561 cccctggcgg gcaatcagta aatcaggttt tacctttgcc agacggcgtt caagttccgg
     7621 atacagccgc tcgataatcg cttcacattc agaccagtgg tggatatctt ccgccatcgt
     7681 gcgttccacg ccgacggatt ttcgcaaccg ttcgctgtta acgtcgcgct cgtcaatccc
     7741 ctgactacgc tcccacaaaa tgcggccaaa tttgccaaag cgtttaagca gcatcaccag
     7801 atcacacttt tgtacatcac cgcaggttcg tagccccatc gcttccagtt ttgccgccga
     7861 gactttgccg acgccgggga tttttgccag tggtaaggtt tgtaaaaatg ccggaacttc
     7921 tgccggcgta atcacaaact ggccgttggg tttattcatg tcggaggcga ttttggcgag
     7981 aaactttacg ggtgccacgc ccgcagacgc cgtcagttgc agctcgttga aaatcgtctg
     8041 gcggatttcc tgggcgatga gggtcgcaga accgtggcaa tggacgctat cggtgacgtc
     8101 gagataagcc tcatccagtg acaacggttc aatacgcgag gtgtagcgcg agaagatttc
     8161 gcggatatga tttgaggctt ctttgtaggc gtcaaagcgc cccggaagca aggtgagatg
     8221 cgggcataat ttgagcgcca tccctgtcgg catagcgcta cgtacgccaa atttacgcgc
     8281 gggataattg gcggtgctga tcaccccccg acgttcgcgg ctgccgccaa tagcaatagg
     8341 gatatcgcgc agggcgggat tgtcgcgcat ctccactgcg gcgaaaaagc agtccatatc
     8401 cacatgaatg attttacgca ttactcacct ctcaacactg gtaaagtata cagtgatttc
     8461 agggtttgag aaatgcgtaa agattcagca tttagcgcgt tcgctgcgaa agcacctgct
     8521 gcttatccag cttttgcacc agcggctgca ccactttatc cccatgctga ccaaagtatt
     8581 gatacagcgt atcggacgca cttttggtca gcaccataat ctcgatgcgc cgattacccg
     8641 cactttgcgg attcttcgcg tccagcagca tctggtccgc cattgcgctt acctgcatca
     8701 ctttatcttc cggcattccg gcctcttcca gcacccgacg agccgaaaac gcgcggtcac
     8761 ccgaaaggtt ccagttgttg tagatattgt ttttgtaggc catcgcatcg gtatgcccgg
     8821 taataataat tttattatcg agcgagtcga acactggcgc gagctccacc agcagcgttt
     8881 taaagaacgg cataatcttc gcgctgccgc gttcaaacat attgcggttc tggtcatctt
     8941 taatcagcac gcgcaatccc tggggaacaa tctccatctc cagattcgct tccatatgcg
     9001 catcacgggc aatggtgttg atactggttg ccagctcgcc aagctcggtg gcggatttct
     9061 tcttcagcag cgccgttttc tcgttgacgt cgcgcgcttt tttctccgtc tcttccggca
     9121 ccgccacggt ggcaggtttt ggcggatgag acgggctgat tttatttaac ggcgacagcc
     9181 caccaccgtt aaaaatcgac tgcccgtgca gcgcggcgat aatgctttca cgctcggatt
     9241 tactgacgct gttgacaatc aactttattg tcgtcaccaa aggggccgag cgtatttccg
     9301 aggtttctgc ccgctttacc ttagacgcga tgcccggcaa acagatggcg attgacgccg
     9361 atcttaacgc cggattgatc aaccaggcgc aggcgcaaac ccggcgtaaa gatgttgcca
     9421 gcgaggccga tttctacggc gcgatggacg gggcatcgaa gtttgtgcgc ggggacgcca
     9481 tcgccgggat gatgattctg gcgatcaacc tgatcggcgg cgtctgtatc gggatcttca
     9541 aatacaacct gagcgccgat gctgccttcc agcagtatgt gctgatgacc atcggcgacg
     9601 gcctggtggc gcagatccct tccctgctgc tctccacggc ggcggcgatt atcgtcaccc
     9661 gcgtcagcga caacggcgat atcgcccatg acgtgcgcca ccagctgctg gcaagcccgt
     9721 cggtgctcta caccgctacc gggattatgt ttgtgctggc gatggtgccg ggaatgccgc
     9781 atctgccgtt tttgctgttc agcgccctgc ttggctttac cggctggcgg atgagcaaac
     9841 gcccgcaggc ggcggaagcg gaagagaaaa gtctcgaaac gctgacccgc accatcaccg
     9901 aaaccagcga gcaacaggtc agttgggaaa ccattccgct gatcgagccc atcagcttaa
     9961 gcctcggcta caagctggtg gcgctggtgg acaaagccca gggcaacccg ctcacccagc
    10021 gtattcgcgg cgtgcgacag gtgatttctg acggcaacgg cgtgctgctg ccggagatcc
    10081 gcattcggga aaacttccgc cttaagccca gccagtacgc tattttcatc aacggcatta
    10141 aggctgatga agcggatatt ccggcggata aactgatggc cctgccctcc agcgaaacct
    10201 acggcgagat tgacggcgtg ctggggaacg acccggcgta tgggatgccg gtcacctgga
    10261 tccagcctgc gcagaaggcg aaggcgctga atatggggta tcaggtgatc gacagcgcca
    10321 gcgtgattgc tacccatgtg aacaagattg tgcgcagcta tattcctgat ttgtttaact
    10381 atgatgatat tacgcagttg cataaccgtt tggcgtcgat ggcaccgcgc ctggcggaag
    10441 atttaagcgc ggcgctcaat tacagccagt tgctgaaagt gtaccgggcg ctgctgaccg
    10501 aaggcgtttc cctgcgcgat atcgtcacca tcgccaccgt gctggtcgcc agtagcgcgg
    10561 tgaccaaaga tcatattctg ctggcagctg atgtgcgcct ggcgctgcgg cgcagcatta
    10621 cccatccgtt cgttcgcaag caggagctga cggtgtatac gctgaataat gaactggaaa
    10681 atctgctgac caatgtggtg aatcaggcgc aacagggcgg gaaagtgatg cttgacagcg
    10741 tgccagtgga cccgaatatg ctcaaccagt tccagagcac gatgccacag gtgaaagagc
    10801 agatgaaagc ggcggggaaa gacccggtgc tgctggtgcc accgcagctg cgccctttgc
    10861 tggcgcgtta tgcaaggttg tttgcgccgg ggctgcatgt gctgtcgtat aacgaagtgc
    10921 cggatgagct ggagttgaag attatggggg cgttgatgta ggcatgataa gacgcgtcag
    10981 cgtcgcatca ggcatcggtg cataaccgcc ggatgcggcg tgaacgcctt atccggccta
    11041 ccgttcaggc gcaggtttgt aggcatgata agatgtgcca gcgtcgcatc aggcatccgc
    11101 gcacaattgc cggatgcggc acaagtttgt aggcacgata agacgcgcca gcgtcgcatc
    11161 aggcatctgc gcataattat aaaatgatac gctcgccagc atttatatcc gttacgtccc
    11221 ccgcccaatc gatgggatat aaccctcgct cgacatcgcg atggaacgtt gagaatggcc
    11281 aatcactcac ttgctttacc caaccatgct ttactggatt tatataaata taatcaacat
    11341 gatgcctata atctttggta ttgcggatgg tgtgctccca aaaacgtggt tgccagatat
    11401 ttttcaatcc acaagcatgg gtaaattgct ttttaatttc ccgccagcga gaggaaaaat
    11461 catcatcgcc ttcaggtaat gtccatatgc aatgcatatg ctctggcaaa acgacccagg
    11521 cgttgatttc aaaaggcctg tctcgcttaa ctttaataat ggcgttacgg agcatctggt
    11581 actgggtggt caagagttgg cttcgacgat tgcgtaaatt caccgtgaaa aaccaggttc
    11641 cccccttgat gtaataacga cgatattcag acatggcgac tcctttttct caacggtaag
    11701 aagaaaagga aaggtcggca atgaggtgat ggaataacag gaaagcggct cgcaaaaacg
    11761 cacagatttg taggcatgat aagacgcgtc agcgtcgcat caggcatctg cgcaccaccg
    11821 ccggatgcgg cgtaaacgcc ttatccgtcc tacaaaatcg tctcttccgt caatatccgc
    11881 cgcgcgccca aaaaatgatc ctgccagaaa ggttcagcta accgacttac ccgaatggtt
    11941 tccccggtgc gcggtgactc aataaactga ccgtcgccca gatacacccc catatggtcg
    12001 gcaatctcgc ggctgtggat atggaaaaac agcaaatctc cccggcgcag gtcgttgttc
    12061 gccacaatcg ttgcccggcg atagtgatac atctcattgg ccgtacgtgg gagcttcgcc
    12121 tcgaggatct tgttgtaggc ataaaaaact aacccgctac agtcgaagcc ttgatcgggc
    12181 cgcgtgccgc cccagacgta aggcttgcca agttgctgtt ccaggcggtg aatcgccact
    12241 tcggtgatgt tatgcagatg gtcgctgctt aagaaatgtt tattctccac cagcgcctgc
    12301 cagcgtctgt cactcgcctt gagcggtgca ggaaaccatt ctggatgctg ttttatctgt
    12361 tcgcggttgt gctggcgtag cgcccttctg ctttcggcat tgccttccac aatatagctg
    12421 gcctgttttt tcagatgggt ttggtactgt tttaataaac gcgcacggtt ttgcatccgc
    12481 tggcgggcgg cgtagctgaa agagatatgt gatgtggtgg cgctggcaaa tgaagaaaac
    12541 ggcaagcaaa gcaacagtaa tcccgacaac acaaaagagg gaatcgacgg caaggacatt
    12601 ctcaataatg gctggtcatg cattgatgtt gcagtgtatc ccggcccgta ccagaacaaa
    12661 cttaataaac cattattata aaattaataa atatatttca gttaattatg taaaaacatc
    12721 cgtttattaa attactcatg gattgtcgct gttgctcatt tgagctacaa ttcaagctga
    12781 ataaatatac agcacaggag ataccccaat ggctgctaac gcgtttgttc gcgcccgaat
    12841 cgatgaagat ctgaagaatc aggcggcgga cgtactggcc gggatggggc tgaccatctc
    12901 tgacctggtt cgcataaccc tcacaaaagt cgcgcgtgaa aaggcattgc cgtttgattt
    12961 acgcgagcct aatcaattaa ccattcaatc aattaaaaac agcgaagctg gcgttgatgt
//
LOCUS       DAPHBJ010000125        12986 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.126, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000125 DAPHBJ010000000
VERSION     DAPHBJ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.126"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..446
                     /locus_tag="RHZ76_003127"
     CDS             <1..446
                     /locus_tag="RHZ76_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDV2813720.1"
                     /translation="FEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTDS
                     NMANVNFNNANLSNCHFNCSVLTKAWMFNIRLYRVNFDEASVQGMGITILRGEENISI
                     NSDTLVTLQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(600..2249)
                     /locus_tag="RHZ76_003128"
     CDS             complement(600..2249)
                     /locus_tag="RHZ76_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HDV2813721.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2401..3750)
                     /gene="ghxP"
                     /locus_tag="RHZ76_003129"
                     /gene_synonym="yjcD"
     CDS             complement(2401..3750)
                     /gene="ghxP"
                     /locus_tag="RHZ76_003129"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HDV2813722.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4296..4760)
                     /gene="soxR"
                     /locus_tag="RHZ76_003130"
     CDS             complement(4296..4760)
                     /gene="soxR"
                     /locus_tag="RHZ76_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HDV2813723.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            4846..5169
                     /gene="soxS"
                     /locus_tag="RHZ76_003131"
     CDS             4846..5169
                     /gene="soxS"
                     /locus_tag="RHZ76_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HDV2813724.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5172..6758)
                     /gene="pdeC"
                     /locus_tag="RHZ76_003132"
     CDS             complement(5172..6758)
                     /gene="pdeC"
                     /locus_tag="RHZ76_003132"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HDV2813725.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGDYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7188..7469
                     /locus_tag="RHZ76_003133"
     CDS             7188..7469
                     /locus_tag="RHZ76_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HDV2813726.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(7568..8104)
                     /gene="ssb1"
                     /locus_tag="RHZ76_003134"
     CDS             complement(7568..8104)
                     /gene="ssb1"
                     /locus_tag="RHZ76_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HDV2813727.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            8359..11181
                     /gene="uvrA"
                     /locus_tag="RHZ76_003135"
     CDS             8359..11181
                     /gene="uvrA"
                     /locus_tag="RHZ76_003135"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDV2813728.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11216..11572)
                     /locus_tag="RHZ76_003136"
     CDS             complement(11216..11572)
                     /locus_tag="RHZ76_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDV2813729.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(11576..11992)
                     /locus_tag="RHZ76_003137"
     CDS             complement(11576..11992)
                     /locus_tag="RHZ76_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HDV2813730.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12103..12816)
                     /gene="aphA"
                     /locus_tag="RHZ76_003138"
     CDS             complement(12103..12816)
                     /gene="aphA"
                     /locus_tag="RHZ76_003138"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HDV2813731.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDIGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         3343 a         3410 c         3195 g         3038 t
ORIGIN      
        1 actttgaaca tgctgatctg tctcgcgtca atctgaacaa agcaatcctc cagaatataa
       61 acttaattga tagcaaactc ttttgtacgt ggctaacaaa tacattcctc gaaatggtta
      121 tatgtaccga ctctaatatg gctaatgtta attttaataa tgccaattta agcaattgcc
      181 atttcaactg ttctgtttta acaaaagcct ggatgtttaa tatccgtctc tatcgtgtta
      241 atttcgatga ggctagcgtc cagggaatgg gtattaccat tctccgtggt gaggaaaata
      301 tctccattaa tagtgatacc ctggtaacac tacagaaatt ctttgaagaa gattgtacct
      361 ctcatactgg catgtcacaa actgaggata atattaatgc agtcgctatg aagattactg
      421 cagatattat gcaacacgca gattgaggtt aagtcgaaga acagccaatg aattgacttt
      481 acaagagaga taacttattt gttttagaat catgaatgtt agcccgagct aacctgttaa
      541 aggtgcaaaa gattgcacct ttttcagatt acctcgggcc tggttacgtc gctttcggct
      601 tactgatttt cctcaattag caacgcttca agcaaatcga gatcgtgcag taatttttgc
      661 agcgtttcgt tgctgatctc ccgcgtggcg cgcaggtggt aaagttcagc acgttcagaa
      721 cgcaatgccg ccagacggaa gcgacgctca aggttctctt cctgcacgga actttcaacg
      781 tcgttacgtc catcggcgcg acgacgcagg ttaccaatga cgcgagaact gacctccgta
      841 agcagctggt tatcgatatt ctcttcggta tccgccgcca gacgctcctc cattttctgg
      901 atcgccacaa tcgccacttc tgccgttgcc gctcgcgcaa tacgttcctc tttcaattgt
      961 tgcgaatggt ccgcgacttc aatgtgttgt agcagaatag gcaacatcac cacgccgaca
     1021 aacagcgaga agagaatgac gccagccgcc aggaacacca gctcatagcg cgccgggaag
     1081 acgttaccat ccggcaagag cagcgggata gagagcacac cggccagagt gatcgcccca
     1141 cgcaccccgg cgaaagacgc gatcaggatt tctcgtgtgg tccacgaacc aaactccatc
     1201 ggcttcttct tcaggaagcg gttgctgaac tttttcatcg tccacagcca gccgaaacgg
     1261 accagcatca gcgccgcata tatcagaata atatcggtaa acagcatcca gatttcgacg
     1321 ttagggtcga tttctgccgc catcagcgac gtctccagaa tacccggcag ctgcagacct
     1381 aacagcagga acaccatgcc gttaaagaca aattccagca tcgcccaggt gctgtttgca
     1441 cgcaggcgca ttgccagcgg cgcacggcgc atcacaccgg agcgggtgat ggtcatcccg
     1501 gcggcaaccg cagcgaggat gcccgaaacg ccaatatgtt cggcaatcag ataagaagcg
     1561 aatggcagca agaacagcag aacgatctgc gtcgcgggtt catcaccgcc ccagcggctg
     1621 aggaatcgca gcgagcgacc atacagccag ctcaccacaa aaccagcgag aataccgcca
     1681 atggctactt tcataaattc gaccgttgca ccgccaacgg tgaagatcat cgtccccatc
     1741 gccactgcca cggcaaactt caacgacacc aggccggagg cgtcattcat caacgcttcg
     1801 ccctgcaaaa tgcccatgat ttttttcggg atgcgccctt caccaacaat cccggagagc
     1861 gccacagcat cggtcggaga aagcaccgcc gccagcgcaa aggcggggat cagcggaatg
     1921 cccggcacca cccagtaaat aaggaagcca atgccgacca cggtgaccac caccagcgcc
     1981 agtgcgaggc cgaaaatctc tcgaccatgt tcaagaaatt cacgggtcgg cgttttccag
     2041 ccatcagcga acagcaacgg cgggataaac aagactaaaa agagttcagg atcaaactcc
     2101 acatgcaaac caaacgtcgg ccacgccagt agcgcaccga tggcgatttg cataagcgga
     2161 agcgggatct gaaagggcat gacacgagtg accaccccgg acagcgagac cacgagggtc
     2221 attatcagta tggtgaagaa gatttccatg cgttccctgt ttgcgatgtt tttttatgac
     2281 ctgagaaaag cgttatgcct gaattctcat tttttcagaa tatcgcaaaa gctcacacaa
     2341 tgtgcggaga aaataaaaac gggcagccat tgccacccgt tttcagaatc cgatgaaaga
     2401 ttagatagcc cagccacccg catagaaggt caccagcgcc acggcgataa cgaccgtacc
     2461 gatgttcaac ttgcgccatt cgccggaaac cagacgccca atcaccagag tcgcgaagcc
     2521 gatcatgatg cctgttacga tgttacaggt cagcacgatg aataccgccg taaccagacc
     2581 cgccatcgca tcaacaaaat cagcaaagtc gattttcgcc acgttgctca gcatcagcag
     2641 gccaacgtac atcagcgccg gagccgttgc ataccccgga acgaggtaag agagcggaga
     2701 gaggaacaga atcagcagga acagcacgcc aacggtgata gccgtcaaac cggttttacc
     2761 gcccgccgcc gtacccgccg cagactcgat gtataccgct gccggagctg cacccaccag
     2821 gccagagaaa acgctgctca tggagtcagt ggtcagtgct ttcccaccgt cgataatttg
     2881 cccatcttta tccagcaggt tcgcctggcc ggcgacggca cggatagttc cggtggcatc
     2941 aaatactgcc gtcattacca gcgccagaac gcttggcagg actacaggat tcagcgcgcc
     3001 cataatgtcc aggctgccaa tcagtgagtt gccgttttca tcgctcaatg aaggcatggc
     3061 gaaaacgccg gagaaatgga cgttaggatc gaagatcaaa ccgacaattg agataccgat
     3121 aatggtcagc agaatgccac cagggacttt cagtttttcc aggccgatga tcaccgccag
     3181 acctaccagt gacataatca ccgggaaggt cgcgaaatca cccagcgcaa cgggcagacc
     3241 atcaagcggg tttttaatca ccagaccgac accgttagcg gcaatgagca gcaggaacag
     3301 accgatacca atccccgtgc cgtgcgccac accgtgcggc aagttgcgca aaatccagct
     3361 acggataccc gtggcagaaa ttaccgtaaa cagcacaccc atcaggaaca cggcacccag
     3421 cgcgacaggt acgctaatat gttgccccag caccaggctg aatgcggtaa atgccgtcag
     3481 ggagatggcg caaccaatcg ccaacggcag attagcccac agacccatca cgatagaacc
     3541 gagtccggca accagacagg ttgcaacgaa aactgccgca ggcgggaagc ctgctttacc
     3601 caacatacct ggaacgacga tgaccgagta gaccatcgcc agaaacgttg ttaacccggc
     3661 aaccacttcc tgacggacag tgcttccacg ttgtgaaatt ttaaaccagg cgtcgagtga
     3721 accgccggta cgcgctgatg gcgtagacat aagaaaacat cccctgagag tttaattttc
     3781 gtcaagatgc gtggcggaca atcccctgcc agcgaaacgt tatcttcctg ctccaggttt
     3841 gcgacaattt gctgcgtcgc aaaaaagcaa acgtttagct ccgcgcatac aaaacgggtg
     3901 gtaaaaaaag caaacgatta tccggcgatc atagcggctt tttcaaccga agtttgctgc
     3961 ttttttctct tttattgtct tgacgatgaa aaaccaggca attgatgcgc aaacgttatc
     4021 gtcggtgcgc aagattgcag cgttatcgag ccgtataccg ttcaggattg cgataaaaaa
     4081 tcgtctgaga tgacaaaaat attgcggtta aaaagtatac ttttacactg gcgaagaaac
     4141 gtcggtgaca tacaattaaa gcatcaacac caaccggaac ctccaccacg tgctcgaatg
     4201 aggtgtgttg acgtcggggg aaaccctcct gtgtaccagc gggatagaga gaaagacaaa
     4261 gaccggaaaa caaactaaag cgcccttgtg gcgctttagt tttgttcatc ttccagcaag
     4321 cgtgcgccgg taccttcttc tcctaagcgg tcgcccgggt tacgcaacgg gcaatcactg
     4381 cgcgaaaggc agccacaacc aatacatccg tccagttcgt cacgcagcgc cactaaggta
     4441 tgaatgcgcc gatccaactc ttctcgccat tgggacgaaa gctgtttcca ctctttcgca
     4501 cttaacgtat gcccttcggg caacacgcca aacgcttcac caatggtcgc cagcggaatg
     4561 ccaatacgct gagcaatttt gataattgca acatatcgca acacatcacg tttatatcgc
     4621 cgctgattgc cgctgttacg gatactggta atcaaccctt tactttcata gaaatgcagc
     4681 gccgataccg ccacaccgct gcgtttcgcc acttcgccgg gggttagcag cgctttaatg
     4741 cggggtaatt tcttttccat aaatcgcttt acctcaagtt aacttgagga attatactcc
     4801 ccaacagatg aattaacgaa ctgaacactg aaaagaggca gatttatgtc ccatcagaaa
     4861 attattcagg atcttatcgc atggattgac gagcatattg accagccgct taacattgat
     4921 gtagtcgcaa aaaaatcagg ctattcaaag tggtacttgc aacgaatgtt ccgcacggtg
     4981 acgcatcaga cgcttggcga ttacattcgc caacgccgcc tgttactggc cgccgttgag
     5041 ttgcgcacca ccgagcgtcc gatttttgat atcgcaatgg acctgggtta tgtctcgcag
     5101 cagaccttct cccgcgtttt ccgtcggcag tttgatcgca ctcccagcga ttatcgccac
     5161 cgcctgtaat tttattgccc gcgcgttaac tcccgcgcgg gtaattgctc catccattgc
     5221 ataaacacct gcggcggcat cgccttcgca aagaaccatc cctggcaata gcgcacgccg
     5281 cgtttgcgca gccagttaac ctgctcctca gtttcgacgc cttcagcgat cgtttttaac
     5341 cccaggctgt gcgccagctc gatgatgtgt tccgcaatca aatgactggt tttgtgggtg
     5401 gtcagcgttt caacaaacga tttgtcgatt ttcaaaatat cgacattcaa tgatttaagg
     5461 ttatgcaagt tagagtagcc aataccaaaa tcatcaattg ccacttcgta acctgcctgg
     5521 cggaaagcca gaataatcgg cgtcattttg tcaacatcaa gaaatgcatg ctcagtcact
     5581 tcaaatttaa tttgctgcgg acgcaccgcg tattgctctg ttttctgatt gattcgcgct
     5641 atcaaccgtg acgtatgaaa atcggaggcc gacaggttaa tagaaacata gcgatctgca
     5701 tgtgttgcca ggtaatcgcc cagatcgcgg aagacattat caataacata atcagttatc
     5761 tgttctatca tcccctcctt ttctgccagc ggaataaact ctgccggatt cattatttgc
     5821 ccctgctcac caggccaacg taacaacgct tcagcgccga tacatttttc tgttttgata
     5881 tcgattattg gctggtaata aagacaaagt tgatgttttt cgagggcgcg ttgcaattta
     5941 cgtttgggag ataaatagtt ttgtcgaata cgtagccaga gtaatagcag aacaagactc
     6001 cccaaaatac cggcgggcaa cgcaaaaata agatgattat aaaaatgggt tataagacgt
     6061 tgatatgaag tcgcaacaat ggctgcaatt gggcgttttg tcgaataaac tgtcgcatat
     6121 aaatagccat ttctttgtac ggttaaatcc ttcaaatgaa tcagcggaga aaacgttgct
     6181 gccgaggcct ctttgcttaa cgagaaaaag gttttcgtca ccgtatcata cacaccccat
     6241 tgcaatgtcg ggtcatcaga catcacttca ctccagaaga gagggttgat aaccgccaca
     6301 taatttcccc gctgcatata ggtcatttta tagccagaga aaaaaggcgt atcgcggtaa
     6361 taatagatag aaacgttagg ttcacgctta taatcggccg gtgcaatcgt atagccgttt
     6421 acaggcgcta tcagcgatga gcataaaaaa tggttatcac gggcatagat caattcatta
     6481 atataaagat agccacgaat aatattcaac attcgctttt gatgggctgg agtgcaaact
     6541 tgcccctgat agcgttcagc tgcatcgctc actaaatctg cctggcgaat gaccagctcg
     6601 gatttatcca gtgccagttg agcaaaggtt cggagctgat tattcacttc tgattttgcc
     6661 caaaggaagg caatccatag cgaaagaatg atgggaaaga gaactaaaaa gataatgccc
     6721 ggcaacgcca gtaattggtg tcgtgcacga tgactcattg ctatcaattt cctgcctgtt
     6781 tatacccggc cattgcaagg tgaaaaccct gggtaccgac gtctttagat aaatcttacg
     6841 gtataaatag cacttttgcc tgatttgaaa ttgatacctt tctaatcgtc acctggatga
     6901 aaataattca ttgatattca aaattaattt cctacaggaa tgcagaggcg gcgggaagat
     6961 atgcccttaa tgccactgaa aaaccacata atctgtacag caataaaagt cacggcctaa
     7021 tatggctaac aaacaatcaa ttgtgatata gttcacaaaa ttaatgaaac aaacagagtg
     7081 tttcattttt gtgttctgca agccccgcga tagccagagt gtaccacgcc tcccgtgaac
     7141 aacgccgcgc tgtccagggc atcggctctt ttcagaggaa ttgtttgatg gctaccctca
     7201 ccactggcgt ggttcttctt cgctggcaac ttcttagtgc cgtaatgatg tttctggcca
     7261 gcacactcaa catccgtttt cgtcggtctg attatgtcgg gcttgcggtg atcagcagcg
     7321 gtctgggcgt tgtttccgcc tgttggttcg caatggggtt gcttggcatc acaatggcgg
     7381 acatcaccgc tatctggcac aacatcgagt cggtgatgat agaagagatg aatcagacac
     7441 cgccacaatg gccaatgatt ttgacctgat acgtagaagc ctccataacg gaggcttctt
     7501 ttttatggct ggcagatgct ttaatcatcc accttaaaac aatataacct attgttttaa
     7561 tgacaaatca gaacggaatg tcatcatcaa agtccatcgg cggctcgtta gacggcgctg
     7621 ccggagcgga ctgctgcggg cgagactgcg cgccgccgct gaactgattg ccaccctgcg
     7681 gctgctgagg ctgaccccaa ccgccctgcg gctgaccacc accgatatta ccacctgccg
     7741 gagcgccacc accctgacga ccacccagca tctgcatggt gccgccaacg ttcaccacga
     7801 cttctgtggt gtagcgatcc tgaccggatt gatcggtcca tttacgggta cgcagctgac
     7861 cttcgatata aacctgagaa cctttacgca gatattcgct ggccacttct gccagtttgc
     7921 cgaacagcac aacgcggtgc cattcagtct gctctttcat ctcgccggtc gctttatcac
     7981 gccaggattc ggaagtagcc agcgtaatgt tggcaactgc gccaccattt ggcatgtagc
     8041 gtacttccgg gtcctgaccc agattaccaa cgagaataac cttgtttacg cctctgctgg
     8101 ccatgttcgt gtctcctgaa aaaaaatcgt tctgaataag tgtaaacgcg cgattgtacc
     8161 attaccaata gcgcttttac tatgttgtga cctcggttcc gggaaacaaa cctggccaga
     8221 cattgttaca caacactccg ggtaatgcat tccaatactg tatattcatt caggtcaatt
     8281 tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat gccaccgggc aaaaaagcgt
     8341 ttaatccggg aaaggtgaat ggataagatc gaagttcggg gcgcccgcac ccataatctc
     8401 aaaaacatca acctcgttat cccccgcgac aagctcattg tcgtgaccgg gctttcgggt
     8461 tctggcaaat cctcgctcgc tttcgacacc ttatatgccg aagggcagcg ccgttacgtt
     8521 gaatcccttt ccgcctacgc gcggcagttt ctgtcactga tggaaaagcc ggacgtcgat
     8581 catattgagg ggctttctcc tgccatctca attgagcaga aatcgacgtc tcataacccg
     8641 cgttctacgg tggggacaat caccgaaatc cacgactatt tgcgtttgtt gttcgcccgc
     8701 gttggcgagc cgcgctgtcc ggaccacgac gtcccgctgg cggcgcaaac cgtcagccag
     8761 atggtggata acgtgctgtc gcagccggaa ggcaagcgtc tgatgctact cgcgccaatc
     8821 attaaagagc gcaaaggcga acacaccaaa acgctggaga acctggcaag ccagggctac
     8881 atccgtgctc gtattgatgg cgaagtctgc gatctttccg atccgccaaa actggaactg
     8941 caaaagaaac ataccattga agtggtggtt gatcgcttca aggtgcgtga cgatcttacc
     9001 caacgtcttg ccgagtcatt tgaaaccgcg ctggagcttt ccggtggtac cgcggtagtg
     9061 gcggatatgg acgacccgaa agcggaagag ctgctgttct ccgccaactt cgcctgccca
     9121 atttgcggct acagtatgcg tgaactggag ccgcgactgt tttcgtttaa caacccggcg
     9181 ggggcctgcc cgacctgcga cggccttggc gtacagcaat atttcgatcc tgatcgagtg
     9241 atccagaatc cggaactgtc gctggctggt ggtgcgatcc gtggctggga tcgccgcaac
     9301 ttctattatt tccagatgct gaaatcgctg gcagatcact ataagttcga cgtcgaagcg
     9361 ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt tgtacggttc tggcaaagaa
     9421 aacattgaat tcaaatacat gaacgatcgt ggcgatacct ccattcgtcg tcatccgttc
     9481 gaaggcgtgc tgcataatat ggagcgccgc tataaagaga cggaatccag cgcggtacgc
     9541 gaagaattag ccaagtttat cagtaatcgt ccgtgcgcca gctgcgaagg gacgcgtctg
     9601 cgtcgggaag cgcgccacgt gtatgtcgag aatacgccgc tgcctgctat ctccgacatg
     9661 agcattggtc atgcgatgga attcttcaac aatctcaaac tcgcaggtca gcgggcgaag
     9721 attgcagaaa aaatccttaa agagatcggc gatcgtctga aattcctcgt taacgtcggc
     9781 ctgaattacc tgacgctttc ccgctcggca gaaacgcttt ctggcggtga agcacagcgt
     9841 atccgtctgg cgagccagat tggtgcgggc ctggttggcg ttatgtacgt gctggacgag
     9901 ccgtctatcg gcctgcacca gcgtgataac gagcgcctgt tgggtacgct tatccatctg
     9961 cgcgatctcg gtaataccgt gattgtggtg gagcacgacg aagacgcaat tcgcgccgct
    10021 gaccatgtga tcgacattgg cccgggcgca ggtgttcacg gcggtgaagt ggtcgcagaa
    10081 ggtccgctgg aagcgattat ggcggtgccg gagtcgttga ccgggcagta catgagcggc
    10141 aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga atccggaaaa agtgctgaag
    10201 ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc tgacgctgcc ggtgggtctg
    10261 tttacctgca tcaccggggt ttcaggttcc ggtaaatcga cgctgattaa cgacacgctg
    10321 ttccctattg cccaacgcca gttgaatggt gcgaccatcg ccgaaccggc accgtatcgc
    10381 gatattcagg ggctggagca tttcgacaaa gtgatcgata tcgaccaaag cccaattggt
    10441 cgtactccgc gttctaaccc ggcgacctat accggcgtgt ttacacctgt gcgcgaactt
    10501 tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc caggacgttt cagctttaac
    10561 gtccgtggcg gacgctgcga agcctgtcag ggcgacggtg tgatcaaagt ggagatgcac
    10621 ttcctgccgg acatttacgt accgtgcgac cagtgtaaag gtaaacgcta taaccgtgaa
    10681 acgctggaaa ttaagtacaa aggcaaaacc atccacgaag tgctggatat gaccatcgaa
    10741 gaggcgcgtg agttctttga tgccgtacct gcactggcgc gtaagctgca aacgttgatg
    10801 gacgttggcc tgacgtacat tcgcctgggg cagtccgcaa ccaccctttc tggtggtgaa
    10861 gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg gcaccgggca gacactgtat
    10921 attctcgacg agccgaccac cggtttgcac ttcgccgata ttcagcaact gctcgacgtg
    10981 ctgcataaac tgcgcgatca gggcaatacc attgtggtaa ttgagcacaa tctcgacgtg
    11041 attaaaaccg ctgactggat tgtcgacctg ggaccggaag gcggcagtgg cggcggcgag
    11101 atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg aagcttcgca tacggcgcgc
    11161 ttcctcaagc cgatgctgta atcgttaagg ccgctttctg agcggccttt tcctttcaga
    11221 gttgcaccag caatttacgt ttttcttccg gcagcaaatt caccgcctgc tgataagacg
    11281 catccaccag ataatagatt tgcgaatctg gcagcgaacc gtcgagatac acggtgctcc
    11341 agtgcgcttt attcagatgg cggcttggac gcacatcgct gtgctgctga cgtagcagct
    11401 ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg attttctact tctttcacca
    11461 tcgcaaacag tacatcttcc actttgatct gcgtcgcttt ccagtcgtta tgcacgctct
    11521 gttctgcgcc tggttttgcc atgcaatatt gtagcaactc cgaaatggtc attttttact
    11581 ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg gatgcgatgt tcccccagcc
    11641 agatgccttg ccaggtgccg gtctgaatac gccctttatg taccggcaat acaagcgatg
    11701 ttcccagcat tgaggatttg atatgagaag gcatatcgtc tgctccctca tagtcatgct
    11761 cataatttcc gttgtcggga acggtgcgga ggaaaaaacg ctccatgtcg tggcgtacgg
    11821 tgggatcgca gttctcatta agtgtcagag aggcggaggt atgttgcagc aacagatgca
    11881 gtaagccgat gttaacgcgc ggcatatcag ccagctgatt cagaatttca tccgttacca
    11941 gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt ttgataccac atgtgctgct
    12001 ccttgataaa actctcttaa tcagtttgca gcaagacagc gaaaggataa aggtgtgatt
    12061 aaaaaaacag cattcgggag agcatcacgc tctcccgctc tgtcagtatt ctgaattgac
    12121 gatcacctct tcaccaaacg cacccgcttg tggcaagggt ttgtaggtag agttggaggc
    12181 gcgcagaatg cggataccac gagcgccgat atcgcgtgcg gcggtaatat cgttatcgga
    12241 atcgccataa aaaattcgga tatttttatc ctgcagccat tgcgattttg tattttgccc
    12301 tggtttatcg cccgcaaaga ttactggatt catgttggtg gcaggaatat gaaaattatc
    12361 cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta cgaccagtca caaagaagat
    12421 cgcgtcaccg cggcgtacat gcatatcaat cagctggcga gcgacctctt ttggaatgct
    12481 gaattcatcc cagccattgt tcattttttc ccagaacaca ggatttttca gataatcttc
    12541 gctttctggt gagaaggttt ttttgccgcg ccagaagccc gggctggaaa aaagtaccgt
    12601 gtcatcgata tcaaacccca ccgccattgg cggacgccct gcgaggctat tttcaatttg
    12661 tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc ctggcaacgt tagtcccagg
    12721 gttaagcggt gaaggagatg aggccagggc aacagcggaa ctgtttagag cgaacaataa
    12781 gcaaacggca ctgattgcct gtgtgatctt gcgcatattt ttccctaaat agtcagtatg
    12841 ttgaaacttt ttattgtgag atttgttgca aaaaccatct gaccataacg ccagctgccg
    12901 gggaaaggaa ggattatctg cggtttttgt gatgaaaagc aggatgatta tatttaaccc
    12961 agtaaatacg ttcctgtctt tttttg
//
LOCUS       DAPHBJ010000126        12952 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.127, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000126 DAPHBJ010000000
VERSION     DAPHBJ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.127"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(46..153)
                     /gene="rprA"
                     /locus_tag="RHZ76_003139"
     ncRNA           complement(46..153)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="RHZ76_003139"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(339..1451)
                     /gene="ydiK"
                     /locus_tag="RHZ76_003140"
     CDS             complement(339..1451)
                     /gene="ydiK"
                     /locus_tag="RHZ76_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HDV2813732.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIDKPNK"
     gene            1665..4721
                     /locus_tag="RHZ76_003141"
     CDS             1665..4721
                     /locus_tag="RHZ76_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDV2813733.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGLRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVAIVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4718..5128
                     /gene="menI"
                     /locus_tag="RHZ76_003142"
     CDS             4718..5128
                     /gene="menI"
                     /locus_tag="RHZ76_003142"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HDV2813734.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5228..5416
                     /locus_tag="RHZ76_003143"
     CDS             5228..5416
                     /locus_tag="RHZ76_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HDV2813735.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            5964..6332
                     /gene="sufA"
                     /locus_tag="RHZ76_003144"
     CDS             5964..6332
                     /gene="sufA"
                     /locus_tag="RHZ76_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HDV2813736.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6341..7828
                     /gene="sufB"
                     /locus_tag="RHZ76_003145"
     CDS             6341..7828
                     /gene="sufB"
                     /locus_tag="RHZ76_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDV2813737.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            7838..8584
                     /gene="sufC"
                     /locus_tag="RHZ76_003146"
     CDS             7838..8584
                     /gene="sufC"
                     /locus_tag="RHZ76_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDV2813738.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8559..9830
                     /gene="sufD"
                     /locus_tag="RHZ76_003147"
     CDS             8559..9830
                     /gene="sufD"
                     /locus_tag="RHZ76_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDV2813739.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLAHDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGCAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGVR"
     gene            9827..11047
                     /gene="sufS"
                     /locus_tag="RHZ76_003148"
     CDS             9827..11047
                     /gene="sufS"
                     /locus_tag="RHZ76_003148"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HDV2813740.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPGQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKQASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHLVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            11060..11476
                     /gene="sufE"
                     /locus_tag="RHZ76_003149"
     CDS             11060..11476
                     /gene="sufE"
                     /locus_tag="RHZ76_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HDV2813741.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11625..12629
                     /gene="ldtE"
                     /locus_tag="RHZ76_003150"
     CDS             11625..12629
                     /gene="ldtE"
                     /locus_tag="RHZ76_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HDV2813742.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRVTQ
                     "
     gene            complement(12693..12929)
                     /gene="lpp"
                     /locus_tag="RHZ76_003151"
     CDS             complement(12693..12929)
                     /gene="lpp"
                     /locus_tag="RHZ76_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HDV2813743.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         3260 a         3184 c         3503 g         3005 t
ORIGIN      
        1 cattattgga aagagtgagg ggcgaggtag cgaagcggaa aaatgttaaa aaaaagccca
       61 tcgtgggaga tgggcaaaga ctacacacag caattcgttg tttcactcag gggatttcca
      121 tgcttataaa tcaatatgtt gatttataac cgtgagctaa tagtaggcat acggactttt
      181 tgcgtcgatc agattcgtct cattagttta aaaactgtaa agaatttgcg acaggtacta
      241 ccagctgaat ggcggtaagc aggaaaatat ggggaagagc aagcaaatat caattagcgg
      301 gggttaacaa tcaggcggca gagccgcctg atgagaaatt acttattcgg tttgtcgatt
      361 tcgccaagtt cttcgagaat ttcctccggt tggtctgtcg gcggcgggac ttcttccacc
      421 cacgcggcaa acagacgcca ggaaacggct aacagaaccg gaccaataaa cagaccgatc
      481 atcccgaaag caatcaaacc accaataacg ccagagagga tcaggatcag cggtaaatcg
      541 gcacccatgc gaattaacat tgggcggatg acgttatcca gcgtgccaac cacaccgctc
      601 cacactaaca ataccgttcc ccaggtggta tcgccagtcc agtagagcca gataatcgct
      661 ggaatcagta ccggtaacgg gccaagctgg acaaggcaag agaggatcat taacaccgtt
      721 agcaacgttg cataaggtac gccggatacg gcgaggccga taccgccaag cactgcctgt
      781 actaacgccg tcaccaccac acccagcgct accgcgcgga tagcctgtgc cgccagcagc
      841 acggcggcat ctccgcgaac gcctgccaga cgggttgcaa aatggcgaat gccttgtgca
      901 acctgttcac cgcgccagta cagcagggca ctgaagagaa gcatcagcgc acaatgcacc
      961 ataaagcgcc cgatatgcgc cgcttgccca acgaaccagg tggtggtggt gccaatataa
     1021 gggcggactt tcgccatgat cgccgtcccc cccatatcca gcaagttgtg ccagcctgca
     1081 tacagcttcg cgccaatcac cggaatggta ttaagccacg ccagatcggg taacgtcatg
     1141 tcaccgctgg aaatggcttt aattagcggg ccgctgccgt cgacgatact gttaaccagc
     1201 aaagcaatag ggatgataaa caccatcact aataacagcg tcatcaccag aacggcgaga
     1261 gagcggcggc caaacatgat cttttgcaaa cgtaacaata ccggccaggt ggcgataacc
     1321 accgtaccgg cccatgcgaa gccgagaata aagggttgaa caatccacag acatgccaca
     1381 atcatgatgg ctaaaaacag caccgaaagc agaatttgtg cgacatccct gggctgacga
     1441 acatttacca taactacttt tcaccttttt tgtgcgtcaa atcgtcgacg cgacgtgaac
     1501 acgcgaaact cacctgcata atgatgagca atttcagcgg tttttaacag gccgattctg
     1561 cccttaattc tgatgggcgt gttagaaaaa aatgtggtac aacaactcag gcaacacgca
     1621 aacgattaat ctctcatcac agaccgcagg aaagggtcaa tataatgatt ccacagattt
     1681 cccaggcacc cggcgtcgtg caactggtgc ttaatttttt gcaagagctg gagcaacagg
     1741 gttttaccgg cgatacggcg acaagttatg ccgaccgtct gacaatgtcg accgacaaca
     1801 gtatttacca acttctcccc gatgcggtgg tatttccgcg ttcaaccgca gatgtagcgt
     1861 tgatcgcccg tcttgccgcc caggaacgct attcatcgtt gatctttact ccccgcggcg
     1921 gcggcaccgg cactaacggt caggcgctca accaggggat tattgttgat atgtcccgcc
     1981 atatgaaccg catcatcgaa attaaccctg aagagggctg ggtgcgcgtt gaggccgggg
     2041 tgataaaaga ccaactcaat cagtacctga aaccgttcgg ctactttttt gcgccggaac
     2101 tttcgaccag caaccgggca acgctcggtg ggatgatcaa taccgatgca tccggtcagg
     2161 gatcgctggt ctatggcaaa acgtcagatc acgtacttgg cctacgtgcg gtattgttgg
     2221 ggggcgatat tctcgatacg caacctttac ccgtcgagct ggcggaaacg ctgggtaaat
     2281 ccaataccac aatcgggcga atttataaca cggtttatca acgttgccgt cagcaacgcc
     2341 agttaatcat cgacaacttc cccaaactta accgttttct taccggttac gatctgcgtc
     2401 atgtctttaa cgatgagatg accgagttcg acctgacgcg cattctgacg ggttcagaag
     2461 ggacgctggc ctttattacc gaagcgcggc tggatattac gcgcttgcct aaagtgcgcc
     2521 gtctggtgaa tgtcaaatat gactcttttg actctgcgct gcgtaacgcg ccgtttatgg
     2581 ttgaggcgcg ggcgctttcg gtagagacgg tggactcaaa agtgctgaat ctggcgcggg
     2641 aagatattgt ctggcattcc gtcagcgagt tgattaccga tgtgcctgac aaagagatgc
     2701 tcgggctgaa cattgtggaa tttgctggtg atgatgaggc gctgattgat gagcgggtaa
     2761 atgcactctg tgcgcggttg gatgagctga tcgccagtca tcaagcaggt gtcatcggct
     2821 ggcaggtgtg ccgtgagctg gcgggggttg aacgtatcta tgcaatgcgc aaaaaagccg
     2881 ttggtctgct tggcaatgcc aaaggtgccg ctaagccaat tccgtttgct gaggatacct
     2941 gcgtaccgcc ggaacatctg gccgattata ttgctgaatt tcgcgcgctg ctcgacagcc
     3001 acggcttaag ctacggtatg ttcggtcacg tcgacgcagg tgtcttgcac gtccgtccgg
     3061 cactggatat gtgcgatccg caacaagaga ttttgatgaa gcaaatctct gatgacgtgg
     3121 tggcgctgac tgcgaaatat ggtggtttgt tgtggggcga gcacggcaaa ggttttcgcg
     3181 ctgaatacag cccggcgttt ttcggtgagg aactttttgc agaactgcgc aaagtgaaag
     3241 cggcatttga cccgcataac cgactcaacc cagggaagat ttgcccgcca gaaggtctcg
     3301 atgcgccgat gatgaaagtg gacgcggtga agcgcggtac attcgatcgg cagatcccca
     3361 ttgcggtacg ccagcagtgg cgcggtgcga tggagtgtaa cggcaacggt ttatgcttca
     3421 actttgatgc ccgtagtccg atgtgtccgt cgatgaagat cacccagaac cggattcatt
     3481 caccgaaagg gcgcgcaacg ctggtgcgtg aatggctgcg tttgttggcg gatcgcggcg
     3541 ttgatccact caaactggaa caagaactgc ctgaatccgg cgtcagtttg cggacgttaa
     3601 ttgcccgcac gcgcaatagc tggcatgcga ataaaggcga atatgacttc tcacacgaag
     3661 tcaaagaggc gatgtcgggc tgtctggcct gtaaagcgtg ttcgacccag tgccccatca
     3721 aaattgatgt gccggagttt cgctctcgtt ttctgcagct ctatcacacc cgttatttac
     3781 gtccgctgcg cgaccacctc gtcgctacgg tcgagagcta cgcgccgctg atggcacgtg
     3841 cgccgaaaac ctttaacttc ttcattaacc agccgctggt gcgcaaactc tcggaaaaac
     3901 atatcggcat ggttgatttg ccactgctgt cggtcccctc gctacaacaa caaatggtgg
     3961 ggcatcgctc ggcaaacatg acgctggaac agcttgaagc tctcaatgca gagcagaaag
     4021 cgcgcacggt gttggtggtg caggacccct ttaccagcta ttacgatgcg caagtggtgg
     4081 cggattttgt ccgtctggtc gaaaaattag gtttccagcc tgtgttactg ccattttcac
     4141 caaatggcaa agcccagcat atcaaaggct ttcttaatcg ttttgcgaag acggcgaaaa
     4201 agacggcgga tttcctcaac cgtatggcga agctgggtat gccaatggta ggcgtcgatc
     4261 cggcgctggt actttgttat cgcgatgaat ataaactggc actgggcgag gaacgtggcg
     4321 agtttaacgt cttactggcg aatgaatggc tggcaagcgc acttgagtca cagccagtgg
     4381 ctatagtcag cggtgaatca tggtatttct ttggtcactg taccgaagtt accgccttgc
     4441 cgggtgcgcc agcacaatgg gccgcgatat ttgcccgttt tggcgcgaaa ctggaaaatg
     4501 tcagcgtggg ttgctgcggc atggcaggga cttacggaca tgaagcgaaa aaccatgaaa
     4561 attcgctcgg gatctatgag ttatcctggc atcaggctat gcagcgactg ccgcgtaacc
     4621 gctgtctggc gaccggatat tcctgccgta gccaggtaaa acgggttgaa ggcacggggg
     4681 tacgccatcc tgtgcaggct ttactggaga ttattaaatg atatggaaac ggaaaatcac
     4741 cctggaagca ctgaatgcta tgggtgaagg aaacatggtg gggttcctgg atattcgctt
     4801 tgaacatatt ggtgatgaca cccttgaagc gacaatgcca gtagactcgc ggacaaagca
     4861 gcctttcggg ttgctgcatg gaggagcatc cgtggtactg gccgaaagta tcggttccgt
     4921 tgccggttat ttatgtaccg aaggtgagca aaaagtggtt ggtctggaaa tcaatgctaa
     4981 ccacgtccgc tcggcacgag aagggcgggt gcgcggcgta tgcaaaccgt tgcatctcgg
     5041 ttcgcgtcac caggtctggc agattgaaat cttcgatgag aaagggcgtt tgtgctgttc
     5101 gtcacgattg acgaccgcca ttttgtgacg ttgtcaccag aaaagtgtga cgcatatcgc
     5161 ctttggtcat tatctgcaca gtaaacggtg gtatactgtg caggtcttgt ctaaaagcga
     5221 ggatgctatg tctactcaac ttgatcccac ccagctggcc attgaatttt tacgccgtga
     5281 ccagagcaat ctttcacctg cccaatatct caaacgtctg aaacaactag aacttgaatt
     5341 tgctgacctg cttactctct cttctgctga attaaaagaa gaaatctact ttgcctggcg
     5401 tctgggcgtg cattgatcct gacttaatgc cgcaagttcg ccaaatcgac ctggattaac
     5461 ctccgtccag gttcgatgac cttcttaata ctgcttatct accgcctgat attgttcaaa
     5521 aataatacta atcgcagttt gtgttaaaac ggcgggttag ctttatgaga ttattcttat
     5581 cgccccttca agagctaagc cactgagagt gccggagata agcgccggat ggggtagaaa
     5641 cccttaagcc tgtgtcgcac agacttaagg gttttcttat ttctgaacgc tgcacttttt
     5701 tacctatctc ttaaatagtc attttcaata caactttttc tttttcatca cagccttaat
     5761 gctccttaga taataaccat tatctaacaa tgagatacct aattcttagc gtgcctgtta
     5821 acccacacat cagggtctat gcttattaaa agcaggcaaa aagcatgttt tacggtaaag
     5881 cccctgcgtt tgctgggttg aactgataat cattatcact aacatgctgt tatacgctga
     5941 aagcgatgaa gtgaggtaaa tcgatggaca tgcattcagg aacctttaac ccacaagatt
     6001 tcgcctggca aggcttaacg ctgacacccg cagcggcgat acacatccgt gagctggtgg
     6061 caaagcagcc gggtatggtc ggcgtgcgct taggcgtgaa gcaaacgggc tgcgcgggct
     6121 ttggctatgt gctcgacagt gttagcgagc cggacaaaga cgatctgctg tttgaacacg
     6181 acggcgcgaa gctgtttgtc ccgctgcaag cgatgccgtt tattgatggc acggaagtcg
     6241 atttcgttcg tgaaggactt aatcagatat tcaaatttca caaccctaaa gcccagaatg
     6301 aatgtggctg tggcgaaagc tttggggtat aggcggtact atgtctcgta atactgaagc
     6361 aactgacgat gtcaaaacct ggaccggcgg cccgctgaat tataaagaag gattcttcac
     6421 ccagttagcc accgatgagc tggcaaaggg gataaacgaa gaggtggtgc gcgcaatttc
     6481 ggcgaagcgt aatgagccgg agtggatgct ggagtttcgt ctaaacgcct atcgcgcatg
     6541 gctggagatg gaagaaccgc actggttgaa agcgcactac gacaagctga attatcagga
     6601 ttacagctac tactcagcac catcgtgcgg taattgtgac gacacttgcg catctgaacc
     6661 tggcgcggtg cagcaaactg gcgcgaacgc ctttttaagt aaagaggtgg aggcggcgtt
     6721 tgagcagttg ggcgttcccg tgcgggaagg caaagaggtg gcggtggatg ccattttcga
     6781 ctcagtttcg gttgccacta cttatcgcga aaaactggcg gagcagggaa ttattttctg
     6841 ttcctttggt gaggcgatcc acgatcaccc ggaactggtg cgtaaatatc tcggcaccgt
     6901 ggtgccgggg aatgacaact tctttgccgc gcttaatgcg gcggtagcct ctgacggtac
     6961 gtttatttat gtgcctaaag gcgtgcgctg cccgatggaa ctttccacct attttcgcat
     7021 taacgcggaa aaaaccgggc agtttgagcg caccattctg gtggccgacg aagacagcta
     7081 cgtcagctac attgaaggct gttccgctcc ggtgcgtgac agctatcagt tacacgcggc
     7141 agtggtggaa gtcatcatcc ataaaaacgc cgaggtgaaa tattccacgg tacaaaactg
     7201 gttccctggc gataacaaca ccggcggtat tctcaacttc gtcaccaagc gtgctttgtg
     7261 cgaaggcgaa aacagcaaaa tgtcatggac gcaatcagaa accgggtcag cgattacgtg
     7321 gaaatatccc agctgcattt tgcgcggcga taactccatt ggtgagtttt actcagtggc
     7381 gctgaccagc ggtcatcagc aagcggatac cggcaccaag atgatccaca tcggtaaaaa
     7441 caccaaatcg accattatct cgaaagggat ctctgccgga catagtcaga acagttatcg
     7501 cggcttagtg aaaatcatgc cgacggcaac caatgcgcgc aatttcactc agtgcgactc
     7561 aatgctgatt ggcgctaatt gtggggcgca taccttcccg tatgtcgaat gtcgcaataa
     7621 cagtgcacaa ctggagcacg aggcaacgac atcacgtatt ggtgaagatc aactgtttta
     7681 ctgcctgcaa cgcgggatca gcgaagaaga cgccatctcg atgattgtta acggtttctg
     7741 caaagacgtg ttctcagaac tgccgctgga atttgccgtt gaagcacaaa aactcctcgc
     7801 catcagtctt gaacacagcg tcggataagg aataaatatg ttaagtatta aagatttaca
     7861 cgttagcgtg gaagataaag ctatcctgcg cggattaagc ctcgacgttc gtcccggcga
     7921 agttcacgcc attatggggc caaacggttc gggcaaaagt accttatcgg caacgcttgc
     7981 cgggcgcgaa gattatgaag tgacgggcgg cacggttgag ttcaaaggca aagatttgct
     8041 tgcactgtcg ccggaagatc gtgcaggcga aggcatcttt atggccttcc agtatccggt
     8101 ggagattcca ggtgtcagta accagttttt cctgcaaacg gcacttaatg cggtgcgcag
     8161 ctatcgcggc caggaaacgc tcgaccgctt tgattttcag gatttgatgg aagagaaaat
     8221 cgctctcctg aagatgccgg aagatttatt aacccgttcg gtaaacgttg gtttttccgg
     8281 cggcgagaaa aagcgcaacg atattttgca aatggcggtg ctggaaccgg agttatgcat
     8341 tcttgatgag tcggactccg ggctggatat tgacgcatta aaagtggtcg ccgatggcgt
     8401 gaactcgctg cgtgatggca agcgctcatt catcattgtt acgcactacc aacgcattct
     8461 cgactacatc aagcctgatt acgttcatgt gctatatcag ggacgaattg tgaaatccgg
     8521 cgatttcacg ttggtcaaac aactggagga gcagggttat ggctggctta ccgaacagca
     8581 gtaatgcgct gcaacagtgg catcacttgt ttgaagctga agggacaaaa cgctcaccgc
     8641 aagcacagca gcatttacaa caattgctgc gtaccggact gccgacacgt aaacatgaaa
     8701 actggaaata tacgccgctg gaagggctga ccaatagcca gtttgtcagc attgcgggag
     8761 agatatcccc acagcagcga gatgccttag cgttaacgtt agacgctgtg cggctggtat
     8821 ttgtcgatgg acgttacgtg tcagcactga gcgatgcgac tgaaggcagc gggtatgaag
     8881 tgagcattaa cgacgaccgt cagggggtac ccgacgctat tcaggcggaa gtgtttctgc
     8941 atttgacgga aagcctggca caaagcgtga cgcatatcgc cgtgaagcgc ggtcaacggc
     9001 cggcaaagcc attgctgtta atgcatatca cccagggcgt ggcaggtgaa gaggtgaaca
     9061 ctgcccatta ccggcatcat ctggatctgg cggaaggtgc cgaagcaacg gtgatcgaac
     9121 attttgtcag ccttaatgat gctcgtcact tcaccggcgc acggttcact atcaatgtcg
     9181 cagcgaacgc ccacttgcag catatcaagc tggcgtttga aaacccggtc agtcaccact
     9241 ttgcacataa cgatttgttg ctggctcacg atgccaccgc atttagccat agtttcctgc
     9301 tgggtggcgc agtgttacga cacaacacca gtacgcaact caatggcgaa aacagcacgc
     9361 tgcggatcaa tagcctggcg atgccggtga aaaacgaggt gtgtgatacc cgcacctggc
     9421 tggaacacaa taaaggtttt tgtaacagcc gacagttgca caaaactatc gtcagcgaca
     9481 aaggctgcgc ggtatttaac ggtttgatca acgtcgcgca gcacgccatc aaaacggatg
     9541 gtcagatgac caacaataat ctgctgatgg gcaaactggc ggaagtggat acgaaaccgc
     9601 agctggaaat ctatgcagat gatgtgaaat gcagccacgg cgcgacggtg gggcgtattg
     9661 atgatgaaca gatgttctat ctgcgctcgc gcgggatcaa tcagcaggat gcccagcaga
     9721 tgatcattta cgcctttgct gctgaactga cggaagcact gcgtgatgag gggcttaaac
     9781 agcaggtgct ggcccgaatc ggtcaacgac tgccaggagg tgtaagatga ctttttccgt
     9841 cgacaaagtg cgggccgact ttccggtgct ttctcgtgag gtaaacggtt tgccgctggc
     9901 ttatctcgac agcgccgcca gtgcgcagaa accgggccag gtgattgacg ccgaggccga
     9961 gttttatcgt cacggctacg cggcggtgca tcgcggtatt cataccttaa gcgcccaggc
    10021 gaccgagaaa atggagaacg tacgcaagca ggcatcgttg tttatcaacg cccgttcggc
    10081 ggaagagctg gtgttcgtcc gcggcacgac ggaagggatc aatctggtcg ccaatagctg
    10141 gggcaacagc aacgtgcggg cgggcgataa catcatcata agtcagatgg agcaccacgc
    10201 taacattgtt ccctggcaga tgctttgcgc acgcgttggc gcagagctgc gtgtgatccc
    10261 gctcaatccc gacggtacgt tgcaactgga gacgctgcct acgctgtttg atgagaaaac
    10321 tcgcctgctg gcaattactc atgtctccaa cgtgcttggc acagaaaatc cactggcgga
    10381 aatgatcacg cttgcgcacc agcatggcgc aaaagtgctg gtggatggcg ctcaggcggt
    10441 gatgcatcat ctggtggatg ttcaggcgct ggattgcgac ttttacgtgt tctccgggca
    10501 taaactgtat ggccccaccg gaattggcat tctttatgtc aaagaagcct tgttgcagga
    10561 gatgccgccg tgggaagggg gcggttctat gatcgccacc gtcagcctga gtgaaggcac
    10621 tacctggacc aaagcaccat ggcggtttga agccggtaca cccaataccg ggggcatcat
    10681 tggtcttggc gcggcgctgg agtatgtttc ggcgctgggg cttaataaca tagccgagta
    10741 tgaacagaat ctgatgcatt atgcgctatc acagctggaa tctgtaccgg atctcactct
    10801 ctatggccca caaaacaggc ttggcgttat tgcttttaat ctcggtaaac accacgccta
    10861 tgatgttggc agttttctcg ataattacgg cattgctgtg cgtaccggac atcactgcgc
    10921 aatgccattg atggcctatt acaacgtccc tgcgatgtgt cgggcgtcgc tggccatgta
    10981 taacacccat gaagaagtgg atcgtctggt gaccggcctg caacgtattc accgtttgct
    11041 gggataacag ggaggcacta tggctttatt gccggataaa gaaaagttgc tgcgtaattt
    11101 tttacgctgc gccaactggg aagagaaata tctctacatt attgagctgg gccagcgtct
    11161 gccagaatta cgcgacgaag acagaagtcc acaaaatagc attcagggct gtcagagtca
    11221 ggtgtggatt gtcatgcgcc agaatgccca gggaattatt gaattacagg gcgacagcga
    11281 tgcggcgatt gtgaaagggc ttattgcggt cgtctttatt ctctacgatc agatgacgcc
    11341 gcaggatatt gtcaatttcg atgtgcgtcc gtggtttgaa aaaatggcgc tcacccaaca
    11401 tctcacccca tctcgttcac aaggtctgga agcgatgatt cgcgcaattc gcgccaaagc
    11461 cgctgcactt agctaaacta aaaggacagc tttcatccgg ttggctcaca ttgccggatg
    11521 gtcaatgttt acactgacct gcacaggagt gttttggttt caggtgaaca taagcgccat
    11581 ccgacgtttc agcgtgagtc tccggcaaat acaggaggtt tacaatgaaa cgcgcgtctt
    11641 tgcttacact cacgcttatc ggcgctttta gcgccatcca ggctgcctgg gcggttgatt
    11701 atccgctacc accaaccgga agccgactgg ttgggcaaaa tcaaacgtat acggtgcaag
    11761 aaggggataa aaaccttcag gctattgccc gacgttttga tactgcggca atgttgatcc
    11821 ttgaagccaa taacactatc gccccggtgc caaaacctgg tacgacgata actattcctt
    11881 cacaactgtt attacctgat gcaccgcgtc aggggattat cgttaacctt gcagagctgc
    11941 gcctttatta ttatccgccg ggagaaaata ttgtgcaggt ttatccaata ggtattggat
    12001 tgcaggggct ggaaacgccg gtgatggaaa cgcgtgttgg gcagaaaatc cctaacccaa
    12061 cctggacgcc tacggcaggc attcgtcagc gttcgctgga gcgtggcatt aaattaccgc
    12121 cagtcgttcc tgccggacca aataacccgc taggacgtta cgcactgcgc ctcgcgcatg
    12181 gtaatggcga atacctcatt catggtacca gtgcgccgga cagcgtcggt ttgcgcgtca
    12241 gttcagggtg tattcgcatg aatgcaccgg atattaaagc cttgttctcc agcgtgcgga
    12301 cgggaacgcc ggtgaaagtg atcaacgaac cggtgaaata ttccgtggag cctaacggga
    12361 tgcgttatgt tgaagtacat cgaccactat cggcagaaga acagcagaac gttcagacaa
    12421 tgccatacac actgccagca ggctttacgc aatttaaaga caataaggct gtagatcaga
    12481 agttagtcga taaagcgttg tatcgtcggg cagggtatcc ggtttcggtg agcagtggag
    12541 caactcccgc agccagcaat gcgccttcag tagagtcagc gcagaatggt gaaccagagc
    12601 aagggaatat gttacgcgtg acgcagtagc ggtaaacggc agacaaaaaa aatggcgcac
    12661 aatgtgcgcc atttttcact tcacaggtac tattacttgc ggtatttagt agccatgttg
    12721 tccagacgct ggttagcacg agctgcgtca tctttagcag cctgaacgtc ggaacgcatt
    12781 gcgttcacgt cgttgctcag ctggtcaact ttagcgttca gagtctgaac gtcagaagac
    12841 agctgatcga ttttagcgtt gctggagcaa cctgccagca gagtagaacc caggattacc
    12901 gcgcccagta ccagtttagt agctttcatt attaataccc tctagattga gt
//
LOCUS       DAPHBJ010000127        12940 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.128, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000127 DAPHBJ010000000
VERSION     DAPHBJ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.128"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            138..1247
                     /gene="livK"
                     /locus_tag="RHZ76_003152"
     CDS             138..1247
                     /gene="livK"
                     /locus_tag="RHZ76_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HDV2813744.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1295..2221
                     /gene="livH"
                     /locus_tag="RHZ76_003153"
     CDS             1295..2221
                     /gene="livH"
                     /locus_tag="RHZ76_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HDV2813745.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2218..3495
                     /gene="livM"
                     /locus_tag="RHZ76_003154"
     CDS             2218..3495
                     /gene="livM"
                     /locus_tag="RHZ76_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HDV2813746.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3492..4259
                     /gene="livG"
                     /locus_tag="RHZ76_003155"
     CDS             3492..4259
                     /gene="livG"
                     /locus_tag="RHZ76_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HDV2813747.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4261..4974
                     /gene="livF"
                     /locus_tag="RHZ76_003156"
     CDS             4261..4974
                     /gene="livF"
                     /locus_tag="RHZ76_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HDV2813748.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDVLLANEAVRSAYLGG"
     gene            5371..6687
                     /gene="ugpB"
                     /locus_tag="RHZ76_003157"
     CDS             5371..6687
                     /gene="ugpB"
                     /locus_tag="RHZ76_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HDV2813749.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            6785..7672
                     /gene="ugpA"
                     /locus_tag="RHZ76_003158"
     CDS             6785..7672
                     /gene="ugpA"
                     /locus_tag="RHZ76_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HDV2813750.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7669..8514
                     /gene="ugpE"
                     /locus_tag="RHZ76_003159"
     CDS             7669..8514
                     /gene="ugpE"
                     /locus_tag="RHZ76_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HDV2813751.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPSVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8516..9586
                     /gene="ugpC"
                     /locus_tag="RHZ76_003160"
     CDS             8516..9586
                     /gene="ugpC"
                     /locus_tag="RHZ76_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HDV2813752.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9583..10326
                     /gene="ugpQ"
                     /locus_tag="RHZ76_003161"
     CDS             9583..10326
                     /gene="ugpQ"
                     /locus_tag="RHZ76_003161"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDV2813753.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAV
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10313..10753)
                     /locus_tag="RHZ76_003162"
     CDS             complement(10313..10753)
                     /locus_tag="RHZ76_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HDV2813754.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10873..12615
                     /gene="ggt"
                     /locus_tag="RHZ76_003163"
     CDS             10873..12615
                     /gene="ggt"
                     /locus_tag="RHZ76_003163"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDV2813755.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSASAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(12653..12937)
                     /locus_tag="RHZ76_003164"
     CDS             complement(12653..12937)
                     /locus_tag="RHZ76_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HDV2813756.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         2853 a         3308 c         3724 g         3055 t
ORIGIN      
        1 atcactatta tcacaaaaat actgcgctaa acccttaatc agacaggcaa aaacagtgca
       61 gtataaaaaa agaacagtct gatttgttaa cacataaaaa caaagcaata caacatcacg
      121 aatggggatt tttgactatg aaacggaatg cgaaaactat catcgcaggg atgattgcac
      181 tggcaatttc acacaccgct atggctgacg atattaaagt cgccgttgtc ggcgcgatgt
      241 ccggcccgat tgcccagtgg ggcgatatgg aatttaacgg cgcgcgtcag gcgattaaag
      301 acattaatgc caaaggggga attaagggcg acaagctggt tggcgtggaa tatgacgacg
      361 cctgcgaccc gaaacaagcc gttgcggtcg ccaacaaaat cgttaatgac ggcattaaat
      421 acgttattgg tcatctgtgt tcttcttcta cccaacctgc atcagatatc tacgaagacg
      481 aaggtattct gatgatctcg ccgggagcga ccaacccgga gctgacccaa cgcggttatc
      541 aacacattat gcgtactgcc gggctggact cttcccaggg gccaacggcg gcaaaataca
      601 ttcttgagac ggtgaagccc cagcgcatcg ccatcattca cgacaaacaa cagtatggcg
      661 aagggctggc gcgttcggtg caggacgggc tgaaagcggc taacgccaac gtcgtcttct
      721 tcgacggtat taccgccggg gagaaagatt tctccgcgct gatcgcccgc ctgaaaaaag
      781 aaaacatcga cttcgtttac tacggcggtt actacccgga aatggggcag atgctgcgcc
      841 aggcccgttc cgttggcctg aaaactcagt ttatggggcc ggaaggtgtg ggtaacgcat
      901 cattgtcgaa tattgccggt gatgctgccg aaggcatgtt ggtcactatg ccaaaacgct
      961 atgaccagga tccggcaaac cagggcatcg ttgatgcgct gaaagcagac aagaaagatc
     1021 cgtccgggcc ttatgtctgg atcacctacg cggcggtgca atctctggcg actgcccttg
     1081 agcgtactgg cagcgatgag ccgctggcgc tggtgaaaga tttaaaagct aacggtgcaa
     1141 acaccgtgat tgggccgctg aactgggatg aaaaaggcga tcttaaggga tttgattttg
     1201 gtgtcttcca gtggcacgcc gacggttcat ccacggcagc caagtgatca tcccaccgcc
     1261 cgtaaaatgc gggcgggttt agaaaggtta ccttatgtct gagcagtttt tgtatttctt
     1321 gcagcagatg tttaacggcg tcacgctggg cagtacctac gcgctgatag ccatcggcta
     1381 caccatggtt tacggcatta tcggcatgat caacttcgcc cacggcgagg tttatatgat
     1441 tggcagctac gtctcattta tgatcatcgc cgcgctgatg atgatgggca ttgataccgg
     1501 ctggctgctg gtggcggcgg gattcgtcgg cgcaatcgtc attgccagcg cctacggctg
     1561 gagtatcgaa cgggtggctt accgcccggt gcgtaattct aagcgcctga ttgcgcttat
     1621 ttccgcaatc ggtatgtcca tcttcctgca aaactacgtc agcctgaccg aaggttcgcg
     1681 cgatgtggcg ctgcccagcc tgtttaacgg tcagtgggtg gtgggccata gcgaaaactt
     1741 ctctgcctct attaccacca tgcaggcggt gatctggatt gtcaccttcc tcgccatgct
     1801 ggcgctgacg attttcattc gctattcccg tatggggcgc gcctgtcgtg cctgcgcgga
     1861 agatctgaaa atggcgagcc tgcttggcat caacaccgac cgggtgattg ctttgacctt
     1921 tgttattggc gcggcgatgg cggcagtggc tggcgtactg ctcggtcagt tctatggcgt
     1981 cattaacccc tatatcggct ttatggccgg gatgaaagcc tttaccgctg cggtgctcgg
     2041 tgggattggc agcattccag gggcgatgat tggcgggctg atcttgggga ttgcggaggc
     2101 gctctcttct gcctatctga gtacggaata taaggatgtg gtttcatttg ccctgctgat
     2161 tctggtgctg ctggtgatgc cgaccggtat tctgggtcgc ccggaggtag agaaagtatg
     2221 aaaccgatgc atattgcaat ggcgctgctc tctgccgcga tgttctttgt cctcgctggc
     2281 gtctttatgg gcgtgcaact ggagctggat ggcaccaaac tggtggtcga cagtgcttcg
     2341 gatgtccgtt ggcagtgggt gtttatcggc acggcggtgg tctttttctt tcagcttttg
     2401 cgacctgctt tccagaaagg gttgaaaagt gtttccggac cgaagtttat tctgcccgcc
     2461 attgatggct ccacggtgaa gcagaaactg ttcctcgtgg cgctgctggt gcttgcggtg
     2521 gcgtggccgt ttatggtgtc acgtgggacg gtggatatcg ccactctgac catgatctac
     2581 attatcctcg gtctcgggct gaatgtggtt gttggtcttt ctggtctgct ggtgctgggg
     2641 tacggcggtt tttacgccat cggcgcttac acctttgcgc tgctcaatca ctattacggc
     2701 ttgggcttct ggacttgtct gccgattgcc gggttaatgg cagcggcggc gggcttcctg
     2761 ctcggctttc cggtgctgcg tctgcgtggt gattatctgg cgatcgttac ccttggcttc
     2821 ggcgaaattg tgcgcatatt gctgctcaat aacaccgaaa ttaccggcgg cccgaacgga
     2881 atcagtcaga tcccgaaacc gactttcttc ggccttgagt tcagccgtac cgctcgtgaa
     2941 ggcggctggg acacgttcag taatttcttt ggcctgaaat acgatccctc cgatcgcgtc
     3001 atcttcctgt atctggtggc gttgctgctg gtggtgctaa gcctgtttgt cattaaccgc
     3061 ctgctgcgga tgccgctggg gcgtgcgtgg gaagcattgc gtgaagatga aattgcctgc
     3121 cgttcgctgg gcttaagccc gcgtcgtatc aagctgaccg cctttaccat cagcgccgcg
     3181 tttgccggtt ttgccggaac gctgtttgcg gcgcgtcagg gctttgtcag cccggaatcc
     3241 ttcacctttg ccgaatcggc gtttgtgctg gcgatagtgg tgctcggcgg tatgggctcg
     3301 caatttgcgg tgattctggc ggcaattttg ctggtggtgt cgcgcgaatt gatgcgtgat
     3361 ttcaacgaat acagcatgtt aatgctcggt ggtttgatgg tgctgatgat gatctggcgt
     3421 ccgcagggct tgctgcccat gacgcgcccg caactgaagc tgaaaaacgg cgcagcgaaa
     3481 ggagagcagg catgagtcag ccattattat ctgttaacgg tctgatgatg cgcttcggcg
     3541 gcctgctggc ggtgaacaac gtcaatcttg aactgtaccc gcaggaaatc gtctcgttaa
     3601 tcggccctaa cggtgccgga aaaaccacgg tttttaactg cctgaccggt ttctacaaac
     3661 ccaccggcgg caccatttta ctgcgcgatc agcatcttga aggtttaccg gggcagcaaa
     3721 ttgcccgcat gggcgtggtg cgcaccttcc agcatgtgcg tctgttccgt gaaatgacgg
     3781 taattgaaaa cctgctggtg gcgcagcatc agcaactgaa aaccgggctg ttctctggcc
     3841 tgttgaaaac gccttctttc cgtcgcgccc agagcgaagc gctcgaccgc gccgccacct
     3901 ggcttgagcg cattggtttg ctggaacacg ccaaccgtca ggcgagtaac ctggcctatg
     3961 gtgaccaacg ccgtctggag attgcccgtt gcatggtgac gcagccggag attttaatgc
     4021 tcgacgaacc ggcggcaggt cttaacccga aagagacgaa agagctggat gagctgattg
     4081 ccgaactgcg caatcatcac aacaccacta tcttgttgat tgaacacgat atgaagctgg
     4141 tgatggggat ttcagaccga atttacgtgg tcaatcaggg gacgccactg gcaaacggta
     4201 cgccggagca aatccgtaat aacccggacg tgatccgtgc ctatttaggt gaggcataag
     4261 atggaaaaag tcatgttgtc ctttgacaaa gtcagcgccc actacggcaa aatccaggcg
     4321 ctgcatgagg tgagcttgca tatcaatcag ggcgagattg tcacgctgat tggcgcgaac
     4381 ggggcgggga aaaccacctt gcttggtacg ttatgcggcg atccgcgcgc caccagcggg
     4441 cgaattgtgt ttgatgataa agacattacc gactggcaga cggcgaaaat catgcgcgaa
     4501 gcggtagcga ttgtcccgga agggcgtcgc gtcttctcgc ggatgacggt ggaagagaac
     4561 ctggcgatgg gcggcttctt tgccgaacgt gaccagtttc aggagcgcat aaagtgggtg
     4621 tatgaactgt ttccacgtct gcatgagcgc cgtgttcagc gggcgggcac catgtccggc
     4681 ggtgaacagc agatgctggc gattggtcgc gcgctgatga gcaatccgcg tttgctgcta
     4741 cttgatgagc catcgctcgg tcttgcgccg attatcatcc agcaaatttt cgacaccatc
     4801 gagcagctgc gcgagcaggg gatgactatc ttcctcgtcg agcagaacgc caaccaggcg
     4861 ctaaagctgg cggatcgcgg ctacgtgctg gaaaacggcc atgtggtgct ctccgatacc
     4921 ggcgacgtgc tgctggcgaa cgaagcagtg agaagtgcgt atttaggcgg gtaataacac
     4981 gttgattgat aaggagtcaa aagactcctt tgagacaggt gacaaatgta aaattgcctg
     5041 atgcgctgcg cttatcaggc ctactgggtg agttgcaata tattgaattt gcacgatctt
     5101 gtaggccgga taaggcgttt acgccgcatc tggcatgaaa cgatgagtaa tctgtagagt
     5161 ttgattcaga ccttctattt ttccgcttat ccgtgcccca tctcccattt tccctcaccc
     5221 acgccgtcac cgccttgtca tctttctgac accttactat cttacaaatg taacaaaaaa
     5281 gttatttttc tgtaattcga gcatgtcatg ttaccccgcg agcataaaac gcgtgaattc
     5341 gcgcattcgg tacaacaaga gagataaacg atgaaaccgt tacattatac agcttcagca
     5401 ctggcgctcg gactggcgtt aatggggaat gcacaggcag tgacgaccat tccgttctgg
     5461 cattctatgg aaggggaact gggtaaagag gtggattctc tggcccaacg ttttaacgcc
     5521 gaaaatccgg attacaaaat tgtaccgacc tataaaggca actacgaaca gaatttaagc
     5581 gcggggattg ccgcatttcg taccggcaac gctccggcta ttttgcaggt ttatgaagtt
     5641 ggcaccgcca ccatgatggc atcgaaagcc attaaaccgg tatatgacgt gtttaaagag
     5701 gcggggattc aattcgatga gtcgcagttt gtgccgacgg tttccggtta ctactccgac
     5761 agcaaaacgg gccacttact ctcccagcca ttcaacagct cgacccccgt tctctattac
     5821 aacaaagacg ccttcaagaa agcaggatta gacccggaac aaccgccgaa aacctggcag
     5881 gatctggcgg actatgccgc gaaactgaaa gcctccggta tgaagtgcgg ctacgccagc
     5941 ggctggcagg gctggatcca actggaaaac tttagcgcct ggaacggtct gccgtttgcc
     6001 agcaaaaaca acggctttga cggcacggac gcggtgctgg agttcaacaa gccggagcag
     6061 gtgaaacaca tcgccatgct cgaggagatg aacaagaagg gcgacttcag ctacgtcggt
     6121 cgtaaggatg aatccaccga gaagttctat aacggtgatt gcgcgatgac caccgcctct
     6181 tccggttctc ttgccaacat tcgcgagtac gccaaattta actacggcgt aggcatgatg
     6241 ccttacgacg ccgatgcgaa agatgcgcca caaaacgcca ttatcggcgg agccagcctg
     6301 tgggtgatgc agggtaaaga taaagaaacg tataccggtg tggcgaagtt cctcgatttc
     6361 ctcgcgaagc cagaaaacgc tgccgagtgg catcagaaaa ccggttatct gccaatcacc
     6421 aaagcagcgt atgacctgac ccgtgagcag ggcttttatg agaaaaaccc aggggcggat
     6481 accgcgacgc gtcagatgct gaataagccg ccgttgccgt tcaccaaagg gctgcgtctg
     6541 ggcaacatgc cgcagatccg cgtgattgtg gatgaagagc tggagagcgt gtggaccggt
     6601 aagaagacac cacagcaggc actggatacc gccgttgagc gtggaaatca gttgctgcgc
     6661 cgctttgaga aatcgacgaa gtcttaatca gtgtaatgtc ggatgcgttt cgcttatctg
     6721 acctggcatc gcgtgtaggc cggataagcg aagcgcatcc ggcacagttc aggaattaac
     6781 cgtaatgtca tcatcccgtc cggtgttccg ctcgcgctgg ctgccttatc tgctggtcgc
     6841 gccgcagctc atcatcaccg ttatcttttt tatctggcct gcgggcgaag cgttgtggta
     6901 ctcgctacaa agcgtcgatc cgtttggttt ctccagccag tttgtcggcc tggataactt
     6961 cgtcacgctg ttccatgaca gctactatct cgactccttc tggacgacga taaaattcag
     7021 cacctttgtc accgtcagcg gtttgctggt atcgctgttc tttgcggcgc tggtggagta
     7081 catcgtgcgc ggcagccgtt tctatcaaac cttaatgttg ctgccttacg ccgtggctcc
     7141 cgccgttgcc gccgtattgt ggatcttcct gtttaacccc ggtcgcgggc tgatcaccca
     7201 ttttctcgcg gagttcggct acgactggaa ccacgcgcaa aacagcggtc aggcaatgtt
     7261 tctggtggtg tttgcctcag tatggaagca aatcagctac aacttcctgt tcttctatgc
     7321 cgcgctgcaa tccattcccc gttcgttgat cgaagccgca gccatcgacg gtgcaggccc
     7381 aattcgccgc ttctttaaga ttgcgctgcc gcttatcgct ccggtgagtt tcttcctgct
     7441 ggtggtgaac ctggtgtatg ccttcttcga caccttcccg gtgatcgacg ccgccacgtc
     7501 cggcgggccg gttcaggcca ccacgacgct gatttataag atctaccgcg aaggttttac
     7561 cggactggat ctggcttcgt ctgccgcaca gtcggtggtg ttgatgttcc tcgtcatcgt
     7621 gctgacggtg gtgcagttcc gctatgttga aagcaaggtg cgttaccaat gattgagaac
     7681 cgtccgtggc tgacgatatt cagccatacc atgctgatcc tcgggatcgc ggtgatcctc
     7741 ttcccgctgt acgtggcgtt tgtcgcggcg acgctggata aacaggccgt ctatgccgcg
     7801 ccgatgacgc tcatccccgg cacacatctg ctggaaaaca tccacaacat ctgggtgaac
     7861 ggggtaggca cgaatagcgc gccgttctgg cggatgttgc ttaacagctt tgtgatggcg
     7921 ttcagcatta cgctcggcaa aattaccgtc tcgatgctct cggcatttgc cattgtctgg
     7981 tttcgttttc cgctacgtaa cctcttcttc tggatgattt ttatcaccct gatgctgccg
     8041 gttgaagtac gtatcttccc gacggtggaa gtcatcgcca acctgaagat gctcgacagc
     8101 tacgccggtt taacgctgcc gctgatggcc tcggcgaccg ccacgtttct gtttcgccag
     8161 ttctttatga cgctgccgga tgaactggtg gaagcggcgc ggatcgacgg cgcgtcacca
     8221 atgcgtttct tttgcgacat cgtttttccg ctctccaaaa ccaatctggc ggcgctgttt
     8281 gtgatcacct ttatttacgg ctggaaccag tatttgtggc cgttgttgat tattaccgat
     8341 gtggatctcg gcaccaccgt ggcagggatc aaagggatga tcgctacagg cgaaggcacc
     8401 acggaatgga actcagtgat ggcggcgatg ttgttaacgc ttatcccttc ggtggtgatt
     8461 gttttagtga tgcagcgtgc cttcgtgcgc ggcctggtcg atagtgagaa ataagatggc
     8521 aggactgaaa ttacaggcag taaccaaaag ctgggatggt aaaacccagg tcattaaacc
     8581 gctgaccctt gatgtggcgg atggcgaatt tatcgtgatg gtcgggccgt ctggctgcgg
     8641 gaaatcgacg ctgctgcgca tggttgccgg gctggagcgg gtgacagaag gcgatatctg
     8701 gatcaacgac cagcgcgtga ctgaaatgga gccaaaagat cgcgggattg cgatggtgtt
     8761 ccagaactac gcgctttatc cgcatatgag tgtcgaagaa aacatggcgt gggggctgaa
     8821 aattcgcggc atgggcaagc agcaaattgc cgagcgcgtt aaagaagcgg cgcgcattct
     8881 ggagctggac ggtctgctca aacgtcgccc gcgcgagctt tccggcggtc agcgccagcg
     8941 tgtggcgatg ggccgcgcga ttgtgcgcga tccggcggtg ttcctgtttg atgagccgct
     9001 ctctaacctc gatgccaagc tgcgcgtgca gatgcgtctt gaactgcaac agttgcaccg
     9061 tcgcctgaaa acgacttcac tctacgttac tcacgatcag gttgaagcga tgacgctcgc
     9121 ccagcgagta atggtgatga acggcggcgt tgccgaacag attggcacac cagttgaagt
     9181 ctacgaaaag cccgccagcc tgtttgtagc gagttttatc ggcagtccgg cgatgaatct
     9241 gctgacaggc cgcgtgaata acgaaggcac gcatttcgaa ctggacggcg gtattgagct
     9301 gccgctaaac ggtggctacc gtcagtatgc cgggcgtaaa atgactctcg gcattcgccc
     9361 ggaacatatt gcgctaagct cgcaggcaga aggcggcgta ccgctggtga tggacacgct
     9421 ggagatcctc ggcgcagata acctggcgca cggacgctgg ggcgagcaga agctggtggt
     9481 gcgactggcg catcaggagc gcccgacggc aggcagcacg ctgtggctgc atctggcgga
     9541 aaatcagctg catctttttg atggtgaaac aggacaacga gtatgagtaa ctggccttat
     9601 ccccgcatcg tcgctcatcg tggcggcggt aagctggccc cggaaaacac cctggcggca
     9661 atcgacgtcg gggcaaaata cggtcataag atgatcgaat ttgacgcgaa gttatcgaaa
     9721 gatggcgaga tcttcctgct ccatgacgac aatctcgaac gcaccagcaa cggctggggc
     9781 gtcgcgggtg aactgaactg gcaggattta ctgcgcgtgg atgcgggcag ttggtacagc
     9841 aaagcgttta aaggtgagcc gctgccgttg ctttcgcagg tggcggaacg ctgtcgtgaa
     9901 cacgggatga tggcgaatat cgaaatcaaa cccaccactg gcactggacc attaacgggc
     9961 aaaatggtgg cgctggcggc acgccaactg tgggccggta tgacgccgcc gctgctgtca
    10021 tcgtttgaga ttgatgcttt agaagccgca caacaggcag taccggaact gccgcgcggt
    10081 ttgttgctgg atgagtggcg cgacgactgg cgcgaactga ccgcgcggct gggctgcgtc
    10141 tctattcatc tcaatcataa gttactcgat aaagtgcgag tgatgcagtt gaaagacgcc
    10201 ggactgcgga ttctggttta taccgtcaat aaaccccagc gcgcggcaga gttgctgcgc
    10261 tggggcgtgg attgcatctg taccgatgcg attgacgtga ttggtccgaa ctttacggcc
    10321 caatagtttt caacggaatg tcaggctgcg gcgtgctggg ctgattcagc atgtcgccgt
    10381 ttctcctctc cggcagcata tgctgctgat tcaacgaact atccggatta cggttgctgt
    10441 ttaacatccc gccgttggtg ttgggcagca tttgctgccg ggcgggattt cgctcccccg
    10501 gctgtgactg caacacccgc tgagaattat tgtttatctg gttttctaaa tgctgctgtt
    10561 gcaactgagt ttgcgttttc agttgctgat tcagcatccc tttttgctgg atttgctgcg
    10621 tttgcatctg ggtttgcatc cgctgctggc tgggtatctg ataccccggc tggttagggt
    10681 tgttcagagt attaatgggc tgtgcaaagc cgacaaacgg caggagtgcc gtaagaatca
    10741 gaagtcgttt catcgcgtat cctcctctga agatatcctt taagtttact cgcttcccga
    10801 caaaacgatg attaattcag agttatatac caggcttagc tggggttgcc ccttaatctc
    10861 tggagaataa cgatgataaa accgacgttt ttacgccggg tggccattgc tgctctgctc
    10921 tcaggaagtt gttttagcgc ctccgccgcg cctcctgcgc cgcccgtctc gtatggtgtg
    10981 gaggaagatg tcttccaccc ggtacgcgcg aaacagggaa tggtagcgtc tgtggacgcc
    11041 actgccactc aggtgggggt ggatattctc aaggagggcg ggaatgccgt tgatgccgcc
    11101 gtggcggtgg gctacgcgct ggcggtaacg catccgcagg cagggaatct gggcggtggt
    11161 ggttttatgt taatccgctc gaaaaatggc aataccacgg ctatcgattt ccgcgaaatg
    11221 gcacccgcca aagcgacccg cgatatgttc ctcgatgatc agggcaaccc ggacagcaaa
    11281 aaatcactca cttcgcatct ggcttccggc acaccgggta cggtagcagg tttctcgctg
    11341 gcgctggata aatacggcac catgccgctg aacaaagtcg tgcagcccgc gtttaaactg
    11401 gcacgcgatg gttttatcgt taacgacgcg ctggctgacg atctcaaaac ctacggtagc
    11461 gaagtgttgc cgaatcacga aaacagtaaa gctatcttct ggaaagaggg cgagccgctg
    11521 aaaaagggcg acacgctggt gcaggcgaac ctggcaaaga gcctggagat gattgctgaa
    11581 aacggcccgg acgaattcta taaaggcacg attgcggaac agatcgccca ggagatgcag
    11641 aaaaacggtg gcttgatcac taaagaagat ttagcggcct ataaagcggt cgaacgcact
    11701 ccgataagcg gcgattatcg cgggtatcag gtttactcca tgccaccgcc atcctccggc
    11761 gggatccata tcgtacaaat cctcaatatt ctggaaaact tcgatatgaa gaaatacggc
    11821 tttggcagcg ccgatgcgat gcaaatcatg gcagaagcgg agaaatacgc ctacgccgac
    11881 cgctcggaat atcttggcga cccggatttt gtcaaagtcc cgtggcaggc actgaccaat
    11941 aaagcctatg ccaaatccat tgccgatcaa attgatatca acaaagcgaa gccgtccagt
    12001 gagattcgcc ctggcaagct tgcgccttat gagagtaatc aaactaccca ttactctgta
    12061 gtggataaag acggtaacgc ggtggcggtg acctatacgc tgaacaccac cttcggtacg
    12121 ggtattgtcg cgggcgagag cggtattctg cttaataacc agatggatga tttctccgcc
    12181 aaaccgggcg taccgaacgt ttacgggctg gtgggcggtg atgccaacgc cgtcgggccg
    12241 aacaaacgcc cgctgtcgtc gatgtcgccg accattgtgg tgaaagacgg taaaacctgg
    12301 ctggttaccg gtagcccagg cggtagccgg atcatcacta cagtgctgca aatggtggtg
    12361 aatagcatcg attatggcat gaacgtcgcc gaagcgacca atgcgccgcg tttccaccat
    12421 cagtggttgc cggacgagct gcgtgtcgaa aaagggttta gcccggatac gctcaagctg
    12481 ctggaagcaa aaggtcagaa agtggcgctg aaagaggcga tgggcagtac acaaagcatt
    12541 atggttgggc cggacggtga gttgtacggc gcatccgacc cgcgctcggt ggatgattta
    12601 acggcggggt actaaggtta gcggccctct tcgtgggaag agggctattt tgtcagggca
    12661 agccgaaggt agcctttttt atttcgtaat cctgtagata ttcttccagc ggctttgccg
    12721 ttcccagaga atgaatttca ccactgtctt tatcaataat aaaaggtgcg ttaccagcta
    12781 agcgcgcggc ctcatctcca gtttcgagaa attctcgtgc ttcgaaacag aaataccagc
    12841 cctggctaaa gcgtccatgt agagtaatga cgaccgggag atctgcatca tcaaggtaat
    12901 ggttcgcttt cgcgaatgcg tcgtgataag taatcataat
//
LOCUS       DAPHBJ010000128        12894 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.129, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000128 DAPHBJ010000000
VERSION     DAPHBJ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.129"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            265..846
                     /gene="mdaB"
                     /locus_tag="RHZ76_003165"
     CDS             265..846
                     /gene="mdaB"
                     /locus_tag="RHZ76_003165"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HDV2813757.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            877..1191
                     /locus_tag="RHZ76_003166"
     CDS             877..1191
                     /locus_tag="RHZ76_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDV2813758.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1239..3131)
                     /gene="parE"
                     /locus_tag="RHZ76_003167"
     CDS             complement(1239..3131)
                     /gene="parE"
                     /locus_tag="RHZ76_003167"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDV2813759.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3160..3741)
                     /gene="yqiA"
                     /locus_tag="RHZ76_003168"
     CDS             complement(3160..3741)
                     /gene="yqiA"
                     /locus_tag="RHZ76_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HDV2813760.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGKNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3741..4568)
                     /gene="cpdA"
                     /locus_tag="RHZ76_003169"
     CDS             complement(3741..4568)
                     /gene="cpdA"
                     /locus_tag="RHZ76_003169"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HDV2813761.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4593..5015)
                     /locus_tag="RHZ76_003170"
     CDS             complement(4593..5015)
                     /locus_tag="RHZ76_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HDV2813762.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5016..5645)
                     /gene="nudF"
                     /locus_tag="RHZ76_003171"
     CDS             complement(5016..5645)
                     /gene="nudF"
                     /locus_tag="RHZ76_003171"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HDV2813763.1"
                     /translation="MLKPDSLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5850..7331
                     /gene="tolC"
                     /locus_tag="RHZ76_003172"
     CDS             5850..7331
                     /gene="tolC"
                     /locus_tag="RHZ76_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HDV2813764.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7479..8150
                     /locus_tag="RHZ76_003173"
     CDS             7479..8150
                     /locus_tag="RHZ76_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HDV2813765.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8156..9316
                     /locus_tag="RHZ76_003174"
     CDS             8156..9316
                     /locus_tag="RHZ76_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDV2813766.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9354..10142)
                     /gene="ygiD"
                     /locus_tag="RHZ76_003175"
     CDS             complement(9354..10142)
                     /gene="ygiD"
                     /locus_tag="RHZ76_003175"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HDV2813767.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10285..11058
                     /gene="zupT"
                     /locus_tag="RHZ76_003176"
     CDS             10285..11058
                     /gene="zupT"
                     /locus_tag="RHZ76_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDV2813768.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11116..11286)
                     /locus_tag="RHZ76_003177"
     CDS             complement(11116..11286)
                     /locus_tag="RHZ76_003177"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813769.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(11547..12200)
                     /gene="ribB"
                     /locus_tag="RHZ76_003178"
     CDS             complement(11547..12200)
                     /gene="ribB"
                     /locus_tag="RHZ76_003178"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HDV2813770.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(12304..12452)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12574..12864
                     /gene="ubiK"
                     /locus_tag="RHZ76_003179"
     CDS             12574..12864
                     /gene="ubiK"
                     /locus_tag="RHZ76_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HDV2813771.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT         3245 a         3448 c         3166 g         3035 t
ORIGIN      
        1 ctttcatctt ttacttattc aaagccatat tttcaatccc caaaaaaacc gaaccaaacg
       61 tgtaaattat tccggtgcga gtaagaatta tgaggaatgg ctatcagtat tgtcattttc
      121 agaaaatatt tatcctgcat cggtgagtca gagctagatc agacttttgc taaattcgca
      181 aaagactttg cacattttgc taatttcacc gtaccgctct gtgacgtact atagtcggca
      241 aacgtctcac tttgaggtta aaaaatgagc aacatcctga ttatcaacgg cgcgaaaaaa
      301 ttcgcccact ccaatggtca actgaacgac accctgaccg aagtcgcgga tggcacactg
      361 cgcgacctcg ggcatgatgt tcgcatcgtt cgcgccgaca gcgactacga tgtcaaagcg
      421 gaagtacaaa actttctctg ggctgatgtg gtgatctggc agatgccagg ctggtggatg
      481 ggcgcgccgt ggacagtgaa aaaatacatt gatgatgtat tcaccgaagg tcacgggacg
      541 ctgtatgcca gcgatggtcg tacccgcaaa gatccgtcga aaaaatacgg ttccggcggc
      601 ctggtacagg gcaaaaaata tatgctttct ctgacctgga acgcaccaat ggaagccttc
      661 accgaaaaag atcagttctt ccacggcgtt ggcgttgacg gtgtgtatct gccgttccat
      721 aaagcaaacc aattcctcgg tatggaaccg ctgccgacat ttatcgctaa tgacgtgata
      781 aaaatgcctg atgttccccg ctatactgaa gaatatcgca agcatcttgt ggaaattttt
      841 ggttaactag agctcaggct ttagaaggag ttaaccatgc ttaccgtaat cgcagaaatc
      901 cgtactcgtc ctggtcaaca tcaccgtcag gcggtattgg atcagtttgc taaaatcgtt
      961 ccaaccgtac tgaaagaaga aggttgccac ggctatgcgc caatggtgga ttgcgcagct
     1021 ggcgtgagtt tccagtctat ggcaccggat tctatcgtga tgattgagca gtgggaaagc
     1081 atcgcgcatc ttgaagcgca tctgcaaacc ccgcacatga aggcgtatag cgaagccgta
     1141 aaaggtgacg tgctggagat gaatatccgt attctgcagc cagggattta atcctgcctt
     1201 gtttgcccgg ccatcctgac cgggcaatgt tctttccttt aaacctcaat ctccgccatg
     1261 tcgcctttct cttgcaacca gttgcggcga tcttccgagc gtttcttcgc cagcagcata
     1321 tccatcatcg cgtcagtacg ctgatcgtct tcatcatcga tagtcaactg caccagacgg
     1381 cgagtgttcg gatcaagcgt ggtttcgcgc aattgcatcg ggttcatttc ccccagacct
     1441 ttaaaacgct ggacgttcgg cttgcctttc ttgcgtttta attgctcaag tacgccctct
     1501 ttctcttctt ccgtcagcgc gtaataaacc tctttcccga gatcaatacg gtagagcggt
     1561 ggcagtgcga cgtaaacgtg accgtgtttc accaacgcgc ggaaatgttt tacgaacaaa
     1621 gcgcagagca gcgtggcaat gtgcagacca tcagagtccg catccgcgag gatacagatt
     1681 ttgccgtaac gaagctggct cagatcgtcg ctgtcaggat cgataccgat cgctaccgaa
     1741 atatcgtgca cttcctgcga agccagcact tcgtcggaag agacttccca ggtgttaagg
     1801 atcttacctt tcagtggcat gatcgcctga tattcgcgat cgcgcgcctg cttggcagat
     1861 ccgcctgcag agtcaccttc cacaaggaac agttcggtac ggttaagatc ctgcgcggta
     1921 cagtcagcca gtttgccagg cagcgccggg ccgctggtca gctttttacg caccactttt
     1981 ttagccgcac gcatacgacg ctgggcgctg gaaatcgcca tctctgccag caactccgcc
     2041 gcctgaacgt tctggttcag ccacaggata aaggcatctt tcaccacgcc agaaacgaat
     2101 gccgcgcact gacgcgaaga gagacgctct ttggtctgcc cggcaaactg cggatcctgc
     2161 atttttactg acagcacata ggcgcagcga tcccagatat cttccgccga caactttaca
     2221 ccgcgcggca gaatgttgcg gtattcacag aactcacgca tcgcgtccaa caggccctga
     2281 cgcagaccgt taacatgggt accgccctgc atcgttggga taaggttgac gtagctttcg
     2341 gtcagcagtt caccgccttc cggcagccac agtagcgccc agtccacagc ttcagtatcg
     2401 ccagcgaaat taccgataaa cggtttttct ggcagcgtcg gtaaaccgtt taccgcttcc
     2461 gccaggtaat cattcagacc gtcctgatag caccagcgct gttcggtgtt gttgatctca
     2521 tctttaaagg taatctcaac gccggggcac agtaccgctt tggctttcag cacatgcgtc
     2581 aggcgtgaaa cagaaaatcg cgggctgtca aagaaggttt catccggcca gaagtgcacg
     2641 ctggtaccag tattgcgttt accgcaagtg ccgacaacct gtaaatcctg caccttttcg
     2701 ccattttcaa aggcgatgtt ataaacctga ccatcgcggc gcacgttaac ttctacgcgc
     2761 ttcgacaggg cgttaaccac cgaaatcccc acgccatgca ggccgccaga gaactggtaa
     2821 tttttgttag agaatttacc gcctgcatgc agacggcaaa gaatcagttc aaccgccggt
     2881 acaccctctt ccgggtgaat atccaccggc atcccgcgcc catcgtcaat aacttctaac
     2941 gactggtcag catgtaaaat aacgtccacg cgttttgcgt gacccgccag tgcttcatcc
     3001 acactgttat caatgacttc ttgccccaaa tggttagggc gagtggtatc ggtatacatc
     3061 cccggacggc ggcgaaccgg ctcaagcccg gtgagtacct caatggcatc agcgttataa
     3121 gtttgcgtca tggtttaagt tagcaattcg agttgatcgt cagagatggt gcagaccaag
     3181 aaaatcgacg atcgggttga aataatcttc gaagcccgtg aatgcgtggt tgccgccttc
     3241 tatgacagtc tggcggcagg aagcgtagta cgccaccgcc tggcggtaat ccagcacttc
     3301 atctcccgtc tgttgcagca gccagatcaa atccggcgct tccagcgggt caatctgcat
     3361 gactttgaga tcgtaaatat ggcgtgactc tagcacatat tgctgcccgg tgtaggggtt
     3421 ctcgttctta ccgagatagt ccgtcagcag ttcaaacggg cgcaccgccg ggtttaccac
     3481 cactgcgggc agcataaaac attgtgacaa ccaggtggcg taatatcccc ccagtgacga
     3541 accgacaata cccagcgaat cgccgccatg ttccaggaca atggattcca gcagctctgc
     3601 cgcgtcggaa ggatacggcg gcaactgcgg aatgatcatc tcaacgtcag ggtgatgttc
     3661 cgccagccag ttttttaaca agctcgcttt tgcagagcgc ggcgagctgt tgaaaccgtg
     3721 taaataaaga agcgtagaca tcagtagcct tctgaagcgg tatcaggttg gaaacgtgtg
     3781 tccgccaggc gatgcacctc ggtggtcagc gtgccatcag catgtaactc gagagtacgc
     3841 cagccgggcg cgatggtgtc cagcgtaaag ttggaacagt gcggcttaaa ctgcacacag
     3901 gtcgacggcg ttgccagcag gcggcgaccg ttccagtcga gatccagctc ctgatgaata
     3961 tgaccgcaca gcaagtattt gacgtgcgga aacttcgcca gcacggtatc cagttcgccc
     4021 gcgttacgca gactgtgttg atcgagccaa ctacaacccg caggtagcgg atgatgatgc
     4081 agcagcagca acgtatggcg ttctggcgca tcggccagtt tacgttccag ccactcaagc
     4141 tgaaactcgc tcagctcacc gtgcggcacg ccaaacacct ggctatccag caacaggatt
     4201 tgccattgct caccaataaa cacgcgcttc gccggggaga tacccgcatc ctgtaacgcg
     4261 ctgtacatcg cgggctggaa atcgtggttg ccaggcagcc agacgcaggg cgcacgaaaa
     4321 cttgcgatgc cttcagcgaa atgctgatag gccgcagagg attgatcctg cgctaaatca
     4381 cctgtcgcga caatcaggtc gaattcgtgc tggtgtggac gaatcgcctc cagcaccgcc
     4441 tggtagcttt cccaggtgtt tacccctaac agggcttcgt gcttttgtgc aaacaggtga
     4501 gtatcggtaa tttgtaaaat cctgactctg gcctcaccag ccagaggaag ggttaacagg
     4561 ctttccaaat ggtgtcctta ggtttcacga cgctagtaaa ccggaatcgc catcgctcca
     4621 tgtgctaaac agtatcgcaa ccagtccgct aaaaactggt taatttgatg cttttcgtcg
     4681 cgttgatgca actttttatt aggataatca taccgcgctt tgaagcgaaa aatctgctgg
     4741 cttgaacaca cttcagccac catcgcgtca tgatacagac gcaccgtcat tgacggaagg
     4801 ctccagtaac tgatcgcggg cgcagtctgt tctattgtca ccagggtagt gtatcgggtc
     4861 gattccacaa tcgtcagccg atattgtgcg tttgccacct gatagcttac agtttcgccg
     4921 ggtgcgtcat tgcgcggtaa caaacggcgc aattgtgaaa agttcatctc gcacaggcgc
     4981 atcatttcag gaaagtcagg tgtgtaacgc ttcatttatg cccactcatt ttttaacgct
     5041 tgatgatgca gctgcagcca ttgcaaagcg atgaccgacg ctgcgttgtc gattttcccc
     5101 tcttctaccc actggtatgc ctgttcccgg cttaccacat gaacgcgaat atcttcgttt
     5161 tcatcagcca gaccatgaat accgcttgcg gtcgtggcgt ccacttcgcc caccataatt
     5221 gacgaacgct cactggtgcc tcccgggctt gccaggaaac ttaacaccgg tttggtccgt
     5281 ttgactatca gtcccgcctc ttcaatcgct tcgcgacggg caacatcttc cacactttca
     5341 ccctcttcaa tcatcccggc aaccatctcc agtagccagg gggtttcact ggtgtcgtat
     5401 gcggcaatcc gaatctgctc aatcagcaca acttcatcac gcactgggtc aaagggtagc
     5461 aagactgcgg cgtgaccgcg ctcaaaaatt tcccgccgta cctcatgact catttgcccg
     5521 ttgaataaac gatgacgaaa tctataaaga tctaatgaaa aaaagccgcg ataaagtgtt
     5581 tctcgtgcaa taatttctac atcgtttttg ccaaatgtaa cgggcaggct gtctggctta
     5641 agcattgtta atgtcctggc actaatagtg aattaaatgt gaatttcagc gacgtttgac
     5701 tgccgtttga gcagtcatgt gttaaattga ggcacattaa cgccctatgg cacgtaacgc
     5761 caaccttttg cggtagcggc ttctgctaga atccgcaata attttacagt ttgatcgcgc
     5821 taaatactgc ttcaccacaa ggaatgcaaa tgaagaaatt gctccccatt cttatcggcc
     5881 tgagcctttc tgggttcagt tcgttgagcc aggccgagaa cctgatgcaa gtttatcagc
     5941 aagcacgcct tagtaacccg gaattgcgta agtctgccgc cgatcgtgat gctgcctttg
     6001 aaaaaattaa tgaagcgcgc agtccattac tgccacagct aggtttaggt gcagattaca
     6061 cctatagcaa cggctaccgc gacgcgaacg gcatcaactc taacgcgacc agtgcgtcct
     6121 tgcagttaac tcaatccatt tttgatatgt cgaaatggcg tgcgttaacg ctgcaggaaa
     6181 aagcagcagg gattcaggac gtcacgtatc agaccgatca gcaaaccttg atcctcaaca
     6241 ccgcgaccgc ttatttcaac gtgttgaatg ctattgacgt tctttcctat acacaggcac
     6301 aaaaagaagc gatctaccgt caattagatc aaaccaccca acgttttaac gtgggcctgg
     6361 tagcgatcac cgacgtgcag aacgcccgcg cacagtacga taccgtgctg gcgaacgaag
     6421 tgaccgcacg taataacctt gataacgcgg tagagcagct gcgccagatc accggtaact
     6481 actatccgga actggctgcg ctgaatgtcg aaaactttaa aaccgacaaa ccacagccgg
     6541 ttaacgcgct gctgaaagaa gccgaaaaac gcaacctgtc gctgttacag gcacgcttga
     6601 gccaggacct ggcgcgcgag caaattcgcc aggcgcagga tggtcactta ccgactctgg
     6661 atttaacggc ttctaccggg atttctgaca cctcttatag cggttcgaaa acccgtggtg
     6721 ccgctggtac ccagtatgac gatagcaata tgggccagaa caaagttggc ctgagcttct
     6781 cgctgccgat ttatcagggc ggaatggtta actcgcaggt gaaacaggca cagtacaact
     6841 ttgtcggtgc cagcgagcaa ctggaaagtg cccatcgtag cgtcgtgcag accgtgcgtt
     6901 cctccttcaa caacattaat gcatctatca gtagcattaa cgcctacaaa caagccgtag
     6961 tttccgctca aagctcatta gacgcgatgg aagcgggcta ctcggtcggt acgcgtacca
     7021 ttgttgatgt gttggatgcg accaccacgt tgtacaacgc caagcaagag ctggcgaatg
     7081 cgcgttataa ctacctgatt aatcagctga atattaagtc agccctgggt acgttgaacg
     7141 agcaggatct gctggcactg aacaatgcgc tgagcaaacc ggtttccact aatccggaaa
     7201 acgttgcccc gcaaacgccg gaacagaatg ctattgctga tggttatgcg cctgatagcc
     7261 cggcccccgt cgttcagcaa acatccgcac gcactaccac cagtaacggt cataaccctt
     7321 tccgtaactg atgacgacga cggggcttcg gccccgtctg aacgtaaggc aacgtaaaga
     7381 tacgggttat ctgccgcatt cttccccctt ctcgcttcaa tttcgaccag ccatcctcta
     7441 ttctgatggg tatttaccac tggtcccgga agacaaaaat gaaacggaca aaatccatac
     7501 gccacgcatc gttccgcaaa aactggagcg cacgccatct gacaccagtc gctctcgcgg
     7561 ttgccactgt ttttatgctg gctggctgtg aaaagagtga tgaaacagtg tctctctatc
     7621 aaaatgctga cgactgttca gctgcaaacc caggcaaaag cgccgaatgt accaccgcgt
     7681 acaacaatgc gctgaaagaa gccgaacgta ctgcgccgaa atacgccacc cgtgaagact
     7741 gtgttgctga atttggtgaa ggtcagtgcc agcaggcacc agcccaggct ggcatggcac
     7801 cagaaaacca ggcgcaggcc cagcaatcca gcgggagttt ctggatgccg ctgatggccg
     7861 gttacatgat ggggcgtctg atgggcggcg gcgcgggatt tgcacagcag ccgctgttct
     7921 cctcgaaaaa cccagccagt ccggcttacg gtaaatatac cgacgcgacg ggtaaaaact
     7981 atggcgcagc ccagccaggc cgcaccatga ccgtaccgaa gacggcaatg gcaccaaaac
     8041 cggcgaccac cactaccgtt acccgtggcg gttttggtga atctgttgcc aaacaaagca
     8101 ctatgcagcg tagtgcaacc ggtacctctt ctcgttcaat gggtggctga taccgatgga
     8161 aagagtcagt attaccgagc gcccggactg gcgtgagaaa gcccacgaat acggtttcaa
     8221 ttttcacacc atgtacggcg agccgtactg gtgtgaagat gcttactaca agttgaccct
     8281 cgcccaggtt gaaaagctgg aagaagtcac cgccgaactg caccagatgt gcctgaaagt
     8341 ggtggaaaaa gtgatcgcca gcgatgagct gatgaccaaa ttccgcattc caaaacacac
     8401 ctggagtttt gtgcgtcaat catggctgac gcaccagcca tcgctttatt cgcgtcttga
     8461 tctggcgtgg gatggcactg gcgaacctaa acttctggaa aataacgccg atacgccaac
     8521 gtcactatac gaggcggcgt tctttcagtg gatctggctg gaagatcagc ttaacgccgg
     8581 taacttgccg gagggcagcg accagtttaa cagtctgcaa gaaaaactga tcgatcgctt
     8641 cgttgagctg cgtgaacagt atggcttcca gttgctgcat ctcacctgct gtcgcgacac
     8701 ggtggaagat cgcggaacca ttcagtattt gcaggactgc gcgacggaag ctgaaattgc
     8761 tactgagttc ctctacatcg atgatatcgg gttaggtgaa aaaggtcagt tcacggattt
     8821 acaggatcag gtaatttcca acctgttcaa gctgtatccg tgggaattta tgttgcgtga
     8881 gatgttctca accaagctgg aggatgcagg cgtacgctgg ctggaaccgg cgtggaagag
     8941 cattatctcc aacaaggcac ttctaccgct actgtgggag atgttcccga atcacccgaa
     9001 cctgctgccc gcttattttg cggaagatga tcatccgcaa atggaaaaat atgtggttaa
     9061 accgatcttc tcccgtgaag gcgcaaacgt gtcgatcatt gagaacggca aaaccattga
     9121 agcagcggaa ggtccgtatg gcgaagaagg gatgattgtt cagcaattcc acccgttacc
     9181 gaaattcggc gacagctata tgctgattgg tagctggctg gtgaacgatc aacccgccgg
     9241 aattggcatt cgtgaagacc gtgcattgat cacccaggat atgtctcggt tttatccaca
     9301 tatttttgtt gagtaatcaa tgatgccgga tggcattcgc catccggtaa ttgttaacct
     9361 atctgcaccg acagcatact taaactgccc atttctatac catcgaccgg aatggtaatt
     9421 ggctcctgcc catcccacgc acctaacaca tacaacaacg gcagatagtg ctctggcgtt
     9481 gggttcgata acgcgccacc ttcatggtcg agataattca ccagaggatg ctgctctacc
     9541 ggcccttgcc acgtcagatt cgctttcaca tactcattaa acgacgtcgc ccacggatac
     9601 ggtgaactat caccgtgcca cttcactgtg cgcaggttat gcaccacgtt accgctggcg
     9661 accaacatta ttccttcatc acgcagcgcc gcgagtttgc gccccatttc gaaatgccag
     9721 gcggcaggtt tgctactgtc gatactcaac tgcaccatcg ggatatcggc gtcaggatac
     9781 atcttaatca gcacgcccca ggaaccgtgg tcaaagcccc aggcttcttt atccagcgtc
     9841 accgggatcg gcgctaacag ctcaaccaga cgctgtgcca gcgcaggcga acccggagca
     9901 ggataatgcg tatcgtacag cgcctgcggg aagccaccaa agtcatgaat cgtgggcggc
     9961 gtctccatcg cggtcactcc tgttccacgg gtaaaccagt gagccgaaac caccacaatc
    10021 gcttgcgggc gtggcaatgt catccccaac ttctgccagc tgcgggtata caaattatct
    10081 tccagcacgt tcatcggact accgtgacct aaaaacaatg ctggcatacg tgttgaagac
    10141 atgatgatat ccttaactaa aggtgtcatt ttgatatcct cacaatacgc ttgttcggcg
    10201 gagtaagaac ccggataaca atgatgatga tcatcagtta ttttgacgat ctgcctgaag
    10261 gtgaagattt ataaggagtt gtcgatgtca gtacctctca ttctgaccat actggcgggg
    10321 gcagccacgt ttattggcgc gtttctcggc gttctcgggc aaaaaccctc gaaccgctta
    10381 ctggcgtttt cgctaggatt tgcggcgggg atcatgttgc tcatctcatt aatggaaatg
    10441 cttcctgccg cactagcagc tgaaggaatg tcgcctgtgt tgggttatgg aatgtttatc
    10501 ttcggtctgc ttggctattt tggcctggac cgcatgttgc cacatgcgca tcctcaggat
    10561 ttaatgcaaa aatcggtgca gccgttgcca aaatcgatca agcgcacagc cattctgctc
    10621 actctcggca tcagtctgca taacttcccg gaagggattg ccacctttgt cacggcgagc
    10681 agcaacctgg agctgggatt tggcatcgca ctggccgtcg cgttgcacaa tatccctgaa
    10741 ggtctggcag tggcaggccc ggtttatgcg gcaacggggt ctaaacgcac cgcgattctg
    10801 tgggcgggga tttctggact agcagaaatt cttggtggtg tgctggcgtg gttaatcctc
    10861 ggtagcatga tttccccggt ggtcatggcg gcaatcatgg cggcggttgc aggaattatg
    10921 gtggcgctct cggttgatga attaatgccg ctcgccaaag agattgaccc taataataac
    10981 cccagctatg gcgtactatg tggaatgtca gtgatgggat tcagtttagt gctgctacaa
    11041 acggcgggaa ttggttaaaa acaacatcag aagttgtcca gcgcatggac aacttctgcc
    11101 agcttcacga aagccttatt ctaccttttc agctgtctct tctctcgtac tgtttaagta
    11161 tttgttccgc gcttcaagca gtgcgtctct gtcctggcga aacgcccgac aatagcgttt
    11221 caaatgcagg aaataaccca ccacaaccag agcaatcaat acaatccagt accaggcgat
    11281 aaacatcctt ttacccttaa tatttaacaa tactaaacat ttagcgcata aatttcacat
    11341 atcctttttc ggatatattc atatggtcgc ataataacgg aacaattata tgattttgat
    11401 cataatgaaa aagaatgcct gatattaatg aaattttctc atagaaagag aagcggaatt
    11461 aacatcttgc atagcaggga aaaataaaaa ccgaagcctg ctacaacttc ggtttcttga
    11521 ttaaggcagt aactaagcag cggttttcag ctggctttac gctcatgtgc ctgacggtat
    11581 gccaccaggt cttcaatagt cacgagcgcc atattgtgtt tattggcaaa ctcaatgcac
    11641 tctggtgcac gcgccatcgt gccatcgtca ttagtcagct cacacagcac accagccggt
    11701 ttaaagcctg ccagcgtcat caaatcaata gtcgcttcag tatgaccgcc acgcgtcagt
    11761 acaccacctg cctgcgcgcg aagtgggaaa acgtgacccg ggcgattcag atcagaaggt
    11821 tttgcgccat cggcaatcgc tgcgcgaacg gtcgtaatac gatcagcggc agaaacaccg
    11881 gtagtcacac cttcagccgc ttcaatggtc acggtaaaac cggtgccata ggcgctggtg
    11941 ttattttcta ccatcattgg cagatcaagt tgtttacggc gatcttcagt gatgcacagg
    12001 caaacaatac cgctaccgtg gcgaatggtc agcgccatct gctcaacggt catggtttct
    12061 gccgggaaga tcatatcgcc ttcgttttca cggtcttcat catcaagcac cattacaccg
    12121 cgtccttcac gcagcgcagc cagtgcattt tcaacacgtt cgaaaggcgt accaaaagag
    12181 gaaagtagcg tctgattcat ggtaaaaaaa cctcactaaa attatggtta ccagaatcag
    12241 ggcagtctta ggagtggcgg catatagcca aaataacgtg agcgggtcca tgcccgacag
    12301 aatcgttact ctctcccatc cggactctaa ccgtcggccc cggaattaca ccggatctgc
    12361 tgtcctttga gttcgcaccc aaagcgctcg cgggctttca actgagttga tttaccgccg
    12421 gtggggaatt tcgccccgcc ctgagaataa gcggattcac tataacgcta atgattagcg
    12481 gcagcaacgc atagcttcac ataattctgg tttatgactt acccttatcg cactacaatg
    12541 gcactcaaca cctatcagta cagggaaact acaatgattg acccgaaaaa aattgagcaa
    12601 atcgctcgcc aggttcacga atcaatgcct aaaggaatca gggagttcgg ggaagatgtg
    12661 gagaaaaaaa tccgccaaac cctacaagcg cagctgacgc gcctcgacct tgtaagccgt
    12721 gaagagttcg acgtccaaac gcaagtcctg ttacggactc gtgagaaact ggcgctgctt
    12781 gaacaaagaa tcagcgaact ggaaaatcgc agtactgaga tcaaaaagca gccagatcca
    12841 gagactctcc ccccaacgct gtaaacattt gtctttaatg cactgtaaat tatg
//
LOCUS       DAPHBJ010000129        12790 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.130, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000129 DAPHBJ010000000
VERSION     DAPHBJ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.130"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            56..1570
                     /gene="caiT"
                     /locus_tag="RHZ76_003180"
     CDS             56..1570
                     /gene="caiT"
                     /locus_tag="RHZ76_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HDV2813772.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1601..2743
                     /gene="caiA"
                     /locus_tag="RHZ76_003181"
     CDS             1601..2743
                     /gene="caiA"
                     /locus_tag="RHZ76_003181"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HDV2813773.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2872..4089
                     /gene="caiB"
                     /locus_tag="RHZ76_003182"
     CDS             2872..4089
                     /gene="caiB"
                     /locus_tag="RHZ76_003182"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HDV2813774.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLATHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4163..5716
                     /gene="caiC"
                     /locus_tag="RHZ76_003183"
     CDS             4163..5716
                     /gene="caiC"
                     /locus_tag="RHZ76_003183"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HDV2813775.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLCEESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MVQPPSANDQQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRVGFCYEAEIRDDHNRPLPAGEIGEICIKGIPGKTIFKEYFLNPQATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIAAHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5825..6610
                     /gene="caiD"
                     /locus_tag="RHZ76_003184"
     CDS             5825..6610
                     /gene="caiD"
                     /locus_tag="RHZ76_003184"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HDV2813776.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6616..7206
                     /gene="caiE"
                     /locus_tag="RHZ76_003185"
     CDS             6616..7206
                     /gene="caiE"
                     /locus_tag="RHZ76_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HDV2813777.1"
                     /translation="MSYYAFEGLIPIVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLIGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7415..7810)
                     /gene="caiF"
                     /locus_tag="RHZ76_003186"
     CDS             complement(7415..7810)
                     /gene="caiF"
                     /locus_tag="RHZ76_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HDV2813778.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(8072..11293)
                     /gene="carB"
                     /locus_tag="RHZ76_003187"
     CDS             complement(8072..11293)
                     /gene="carB"
                     /locus_tag="RHZ76_003187"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDV2813779.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGADRIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     CEAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11311..12459)
                     /gene="carA"
                     /locus_tag="RHZ76_003188"
     CDS             complement(11311..12459)
                     /gene="carA"
                     /locus_tag="RHZ76_003188"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDV2813780.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         3000 a         3323 c         3400 g         3067 t
ORIGIN      
        1 aataaaacaa acgggaacgc aaaataaata ttcgttttca cagtggaatt aactcatgaa
       61 gaatgaaaag agaaaaacgg gaatagaacc gaaggttttc tttccgccgt taataatcgt
      121 cggcatactt tgttggctta cagtcagaga tctggatgca gcgaatgtcg ttattaatgc
      181 tgtattcagt tacgtcacca atgtatgggg atgggcattt gaatggtata tggtggtgat
      241 gcttttcggt tggttctggc tggtgtttgg cccgtatgcc aaaaagcgtt taggtaacga
      301 accgccagaa tttagcaccg ccagttggat ctttatgatg ttcgcctcct gtacgtctgc
      361 tgccgtactg ttctggggat cgattgagat ctactactac atctccaccc cgccgtttgg
      421 cttagaaccg aactcgacag gggcgaaaga gttggggctg gcttacagct tgttccactg
      481 gggacctctg ccgtgggcca cttacagctt cctttcagtc gccttcgctt acttcttctt
      541 tgtccgcaaa atggaagtga ttcgccccag ctcgacactg gtgccgctgg taggtgaaaa
      601 acacgccaaa gggttgttcg gtaccatcgt cgacaacttc tatctcgtcg ccttgatctt
      661 cgcgatgggt accagtctgg gccttgccac gccgctagtg accgagtgta tgcaatggtt
      721 gtttggcatt ccgcataccc tgcaactgga cgctatcatc attacctgct ggattatcct
      781 caacgccatt tgcgtcgcct gcggtctgca aaaaggggta cgtatcgcca gtgacgtgcg
      841 tagttacctg agcttcctga tgctgggttg ggtgttcatt gtcagcggtg ccagcttcat
      901 catgaactac ttcaccgatt cggtggggat gttgctgatg tatctgccgc gcatgttgtt
      961 ctataccgat cccatcgcta aaggcggctt cccgcagggc tggaccgtct tctactgggc
     1021 atggtgggtg atttacgcta tccagatgag tatcttcctc gcccgcatct cccgtggtcg
     1081 taccgtgcgt gaactgtgct tcggcatggt gctggggctg acagcgtcaa cctggatcct
     1141 gtggactgta ctcggtagta acactctgct gttgatagat aaaaacatca tcaacattcc
     1201 aaatctgatc gaacagtacg gtgtggcgcg cgccatcatc gaaacctggg ccgctctgcc
     1261 actcagcacc gccaccatgt ggggcttctt catcctctgc tttattgcca ccgttacgct
     1321 ggttaacgcc tgctcttata ccctggcgat gtccacttgc cgcgaagtac gcgatggtga
     1381 agaaccaccg ctgctggtgc gtatcggttg gtcaattctg gttggcatta tcggtattgt
     1441 tctgctggcg ctcggcggcc tgaaaccgat tcaaaccgcc attatcgccg gaggatgccc
     1501 gctgttcttc gtcaacatta tggtgacgct ctcctttatt aaagacgcga aacagaactg
     1561 gaaagattaa ttaaccccca aaatatcaag aggttgaaag atggatttta atttaaatga
     1621 tgagcaggaa ctgtttgtcg ccggtatccg cgaactgatg gccagcgaaa actgggaggc
     1681 ctattttgcc gagtgcgacc gtgacagcgt ctacccggaa cgttttgtca aagcactggc
     1741 ggatatgggt atcgacagtc tgctgatccc tgaagagcac ggtggtctgg acgcggggtt
     1801 tgttactctc gccgccgtgt ggatggagct gggacgtctg ggggcaccaa cctatgtgct
     1861 gtaccagttg ccgggcgggt tcaatacctt cctgcgcgaa ggcacacaag agcagatcga
     1921 caaaattatg gctttccgcg gcaccggtaa gcagatgtgg aactcagcga ttaccgaacc
     1981 gggcgcgggc tccgacgtgg gtagcctgaa aacgacttat acccgtagaa atggtaagat
     2041 ttatcttaat ggtagtaagt gttttattac cagcagcgcc tacaccccgt acatcgtggt
     2101 gatggcgcgc gacggggctt ctccggacaa acctgtctac accgaatggt ttgttgatat
     2161 gagcaaaccg ggcatcaaag tgaccaaact tgaaaagctc ggtctgcgta tggatagctg
     2221 ctgtgaaatc acctttgacg acgtggaact ggacgagaaa gacatgttcg gtcgggaagg
     2281 taacggcttt aaccgcgtca aagaagagtt cgatcatgaa cgtttcctgg tagccctcac
     2341 caactacggt acggcgatgt gcgcctttga agatgcggcg cgctacgcca accagcgcgt
     2401 gcagtttggc gaggctattg gtcgtttcca gttgattcag gaaaaattcg cccacatggc
     2461 gatcaaatta aactccatga aaaacatgct gtatgaagca gcgtggaaag cagacaacgg
     2521 caccatcacc tctggcgatg cagcgatgtg caaatacttc tgcgccaatg cggcatttga
     2581 agttgtggat agcgcaatgc aggtgctggg cggtgtcggg attgcgggca accaccgcat
     2641 cagccgcttc tggcgtgatc tgcgtgtaga ccgcgtctcc gggggatctg acgaaatgca
     2701 gatcctgacg ctgggtcgtg cggtgctgaa gcaataccgc taagtccgtg cgctgcctga
     2761 tgcgacgctg atgcgtctta tcaggcctac gggagcacga atgtaggccg gataaagcgt
     2821 ttacgccgca tccggcagtc atgcgctaca taaatttttc aggagcaaat aatggatcat
     2881 ctacccatgc cgaaattcgg gccgttggcc ggattgcgcg ttgtcttctc cggtatcgaa
     2941 atcgccggac cgtttgccgg gcaaatgttc gcagaatggg gcgcggaagt tatctggatc
     3001 gagaacgtcg cctgggccga caccattcgc gttcaaccga actacccgca actctcccgc
     3061 cgcaatttgc acgcgctgtc gttaaatatt ttcaaagatg aaggccgcga agcgtttctg
     3121 aaattaatgg aaaccaccga tatcttcatc gaagccagta aaggtccggc ctttgcccgt
     3181 cgtggcatta ccgatgaagt actgtggcag cacaacccga aactggttat cgctcacctg
     3241 tccggttttg gtcagtacgg caccgaggag tacaccaatc ttccggccta taacactatc
     3301 gcccaggcct ttagtggtta cctgattcag aacggtgatg ttgaccagcc aatgcctgcc
     3361 ttcccgtata ccgccgatta cttttctggc ctgaccgcca ccacggcggc gctggcagca
     3421 ctgcataaag tgcgtgaaac cggtaaaggc gaaagtatcg acatcgccat gtatgaagtg
     3481 atgctgcgta tgggccagta cttcatgatg gattacttca acggcggcga aatgtgcccg
     3541 cgcatgagca aaggtaaaga tccctactac gccggttgcg gtctgtataa atgtgccgac
     3601 ggctacatcg tgatggaact ggtgggcatt acccaaattg aagagtgctt taaagatatt
     3661 ggcctcgcac atctgcttgg cacgccagaa atcccggaag gcactcagct tatccaccgt
     3721 atcgaatgcc cttacggccc actggttgaa gagaaactcg atgcctggct ggcgacacat
     3781 accatcgcgg aagtaaaaga acgctttgct gaactgaata tcgcctgcgc caaagtgctg
     3841 accgtaccgg aactggaaag caatccacag tatgtggctc gcgaatcaat cactcagtgg
     3901 caaacgatgg atggtcgcac ctgcaaaggg ccgaacatca tgccgaaatt caaaaataac
     3961 cccggacaaa tctggcgcgg aatgccctca catggcatgg acacggctgc cattttgaaa
     4021 aatatcggct acagcgaaaa cgacattcag gagttggtca gcaaaggtct ggccaaagtt
     4081 gaggactaaa cgcgtggcgt cagtcggctt gcctgactag cgcccgccca ataacaaacg
     4141 aacaaaaatg gatagaggtg caatggatat cattggcgga caacatctac gtcaaatgtg
     4201 ggacgatctt gcggacgttt acggtcataa aacggcgctg atttgtgaat ccagcggcgg
     4261 agtcgttaac cggtatagtt atcttgagtt aaatcaggag attaaccgca cggcaaacct
     4321 gttttatacg ctggggattc gcaaaggcga caaggttgca ctacatctcg acaactgccc
     4381 ggaatttatc ttttgctggt tcgggctggc aaaaattggc gcgattatgg tgccgattaa
     4441 cgcccgcctg ttatgcgagg aaagcgcgtg gatcctgcaa aatagccagg cgtgcctgct
     4501 ggtgaccagt gcgcaattct atcctatgta tcaacagatt cagcaggaag atgccactca
     4561 attgcggcac atttgcctga cagatgtggc acttcccgct gatgatggcg tgagttcgtt
     4621 tactcaactg aaaaatcaac aacctgccac cttgtgctat gcaccgccgc tatcgactga
     4681 cgatacggcg gaaattctct tcacctccgg caccacctcc cgaccgaaag gtgtggtgat
     4741 tacccattac aacctgcgct tcgctggata ttactccgcc tggcagtgtg cactgcgtga
     4801 cgatgacgtc tacctgacgg taatgcctgc gtttcatatc gattgccagt gtactgcggc
     4861 gatggcggcg ttttctgccg gggccacctt tgtgctggtc gagaaataca gcgcccgcgc
     4921 cttctgggga caggtacaga agtaccgcgc caccgttacc gaatgtattc caatgatgat
     4981 ccgtacgttg atggtacagc cgccttcagc gaacgatcag caacaccgcc tgcgggaagt
     5041 gatgttttat ctcaacttgt cggagcagga aaaagatgcg ttttgtgaac gcttcggcgt
     5101 tcgcttgctg acgtcttatg ggatgacgga aaccattgtg ggcattatcg gcgatcgtcc
     5161 tggcgataaa cgacgctggc cgtcgattgg tcgggtgggg ttttgctacg aagcggagat
     5221 ccgcgacgat cacaatcgcc cgctcccggc tggtgagatc ggtgaaatct gcattaaagg
     5281 catacctggg aaaaccatct tcaaagagta ctttctcaac ccacaagcca ctgcgaaagt
     5341 gctggaagcc gatggctggc tgcataccgg cgataccgga taccgcgacg aagagggctt
     5401 tttttatttc gtcgatcgcc gctgcaatat gattaaacgt ggcggcgaga atgtctcctg
     5461 cgtggagctg gaaaatatta tcgccgcgca cccgaaaatt caggacatcg tggttgtggg
     5521 tattaaagat tcgattcgcg atgaagccat caaagcattt gtggtgctga atgaaggtga
     5581 aacattgagc gaagaggaat ttttccgctt ctgcgaacaa aatatggcga aatttaaagt
     5641 gccctcttat ctggagatca gaaaagatct gccacgtaat tgctcgggga aaataattag
     5701 aaagaatctg aaataaatga aacagcaggg aactaccctg ccagctaata atcacactct
     5761 aaaacaatat gcattttttg cagggatgtt gtcgtccctg aaaaagcaaa aatggagaaa
     5821 aggaatgagt gaatcattac atctgacccg caatggatca attctggaaa ttacccttga
     5881 tcgtccaaaa gcgaatgcta ttgatgcaaa aaccagcttt gaaatgggcg aagtatttct
     5941 aaatttccgt gacgatccgc aattacgggt cgccattatt accggtgccg gagagaagtt
     6001 cttttccgcg ggctgggatt taaaagcggc agcagaaggc gaagcaccgg atgctgactt
     6061 tggtccgggt ggttttgcgg gattaaccga aattttcaat ctcgacaaac cggttatcgc
     6121 agctgtgaac ggctatgcct ttggcggcgg ctttgaactg gcgctggcgg cagattttat
     6181 tgtttgtgcc gataacgcca gcttcgccct gccggaagcc aaactgggta tcgttcctga
     6241 cagcggcggc gtgctgcgtc tgccgaagat cctgccgcct gccatcgtca atgaaatggt
     6301 gatgaccggc agacgaatgg gcgcagaaga ggcgctgcgt tgggggatag tcaaccgcgt
     6361 ggttagccag gcggaactga tggataacgc ccgcgaactg gcgcagcagc tggttaacag
     6421 cgctccgctg gcgattgcgg cgctgaaaga gatctaccgc accaccagcg aaatgccggt
     6481 agaagaagcg tatcgctata ttcgcagcgg cgtgttgaaa cactatccat cggttctgca
     6541 ttcggaagat gccatagaag ggccgctggc gtttgccgag aagcgcgatc cggtgtggaa
     6601 aggacgttaa caaccgtgag ctattacgcc tttgaggggt taattccgat agttcacccg
     6661 acggcgtttg tccatcccag tgcagtattg attggcgatg tgattgtggg agccggtgtc
     6721 tacatcggcc cactcgcctc actgcgtggt gactacgggc ggttgatcgt gcaagcggga
     6781 gccaatattc aggatggctg cattatgcat ggctactgcg acactgacac catcgttggg
     6841 gaaaacggcc atatcgggca cggagcgatc ctgcatggtt gtgtgattgg tcgcgatgca
     6901 ttggtcggga tgaacagcgt gattatggat ggcgcggtca ttggcgaaga gagcattgtt
     6961 gccgccatga gctttgtcaa agcaggcttt agcggcgaga aacgccagtt gttgataggt
     7021 actccagccc gcgctgtacg cagtgttagt gacgacgagt tacactggaa acggttgaat
     7081 accaaagagt atcaggatct tgtcgggcgc tgtcatgcat cgttacatga aacgcagccg
     7141 ttgaggcaaa tggaggaaaa tcgcccccgt ttgcagggga cgacggatgt gacgccgaaa
     7201 cggtgatctt gccggatgcg gcgtaaacgc cttatccaac tctgctggag agcaaccgta
     7261 ggccggataa gatgcgccag catcgcatcc ggcgatagct gtcggatgcg gcataaacgc
     7321 cttatccaac ccggctggag agcaaccgta agccggataa gatgcgccag catcgcatcc
     7381 ggcgaccagg tcgcacggta tcatcgaaat gacattaacg acgcatactc tttgacaaca
     7441 tcatctgcca cttctgctcg cggttaagtt ccgtgagcgg aacggcagga atacgcggtg
     7501 tctttcttac gccgactaat ttttcccgac tgttattgcg caggcgcgcg taaatttgct
     7561 catcgatatc gaccacttta accagtcgct gacactggca tccccgccct tccagcttat
     7621 tagggatcat cttaacttcg cagcttattt ctgtgacttc cgacagaata taagtcaggg
     7681 tattaaccgc cttgctgtgt tcaatatcga aatgaataga gatctctctt gctatcaccc
     7741 accgattttc agccatcatc cattcggcga ttaacaagta gagtggtttt tcaacatatc
     7801 cttcacacat acgaactcca gtttcacaaa ttaaaagtct ggctatgact cgttttgaag
     7861 caattaaaca aggccatatt aaaattgtgg atatatcgat agtatgcaac ttcaccgtcg
     7921 ataagtgtga gctaaatcct gcttatgtca tcaaaaatat cgattcccca aaagatttac
     7981 ttaatgggca aaaaaaaacg gtcctcatca gagaaccgta ttagttattc gatcaaatta
     8041 gcggatgaaa aatatctgcc atgacacgct attatttgat ctgtgcgtgc atttcctgca
     8101 ccgaaattac tttttcagtc gcatcggcat tcagcgccat cgcggtggca aagccgccgt
     8161 tcagggtggt gtcgtaatgc actttatatt gcagcgcact gcgacgaatc acgcgggagt
     8221 cttcaatcgc acgacggcct gaggtggtgt tgatgatgta ggtatattcg ccattcttga
     8281 tacggtcctg aatgtgcgga cggccttcat gcaccttgtt taccagacgc gggttgatac
     8341 ctgcttcgcc cagcacaatc gccgtgccgt gggtcgcatc cagctcgaag ccctgtttca
     8401 gcagttttgc cgccaggtcc accacgcgtt ctttatcgcc ttcgcgcacg gaaagcagcg
     8461 cacgaccgtg tttcttcatg gtggagttgc tgcccagctg cgctttggca aacgcttcag
     8521 cgaaggtgcg gcccacgccc atgacttccc cggtagagcg catttctggc cctaacagcg
     8581 ggtcaacgcc cgggaattta ttgaacggca gcaccacttc tttcaccgag tagtacggcg
     8641 ggataacttc tttggttacg ccctgctcag ccagcgattt gccagccatc acgcgcgccg
     8701 ccacttttgc cagcggtacg ccggtggctt tggagacgaa cggaacggta cgcgccgcac
     8761 gcgggttaac ttcaatcagg tagacttcgt tgtttttcac cgcaaactgc acgttcatca
     8821 ggccgcgcac ctgcaattcg aaggccagtt tctgcacctg ctggcgcatc acatcctgaa
     8881 tttcctgact taaagtgtag gctggcagcg aacatgcgga gtcaccggag tgcacgcccg
     8941 cctgctcaat atgctccatg atgccgccaa tcagcaccat ttcgccgtcg cagatggcgt
     9001 ccacatccac ttctaccgcg tcatcgagga agtggtccag caacactggc gcatcgttag
     9061 acacgctgac cgccgtctgg aagtagcgac gcaggtctgc ttcgtcatag acgatttcca
     9121 tcgcccgacc gccgagaacg taagacggac gtaccaccag cgggtagcca atctctttcg
     9181 ccttctctac cgccatttcg atagcggtaa cggtggcgtt cgccggttgc ttcagtttca
     9241 ggcggtcaac cgcgtgctgg aagcgttcac ggtcttccgc acggtcgata gcatccgggc
     9301 tggtgccgat aaccggtacg ccagcggctt ccagtgcacg cgccagtttc agcggggtct
     9361 gaccgccgta ctgaacgata acgcctttcg gcttctcaat acgcacgatt tccagcacat
     9421 cttccagggt taccggctcg aagtagaggc ggtcggaagt gtcgtagtcg gtggagacgg
     9481 tttccgggtt acagttaacc ataatggttt cgtaaccgtc ttcgcgcagc gccagcgagg
     9541 cgtgtacaca gcagtagtcg aattcgatac cctgaccgat acggttcggg ccgccgccga
     9601 gaaccatgat tttttcacgg tcggtagacg gattcgcttc gcactcttct tcataagtgg
     9661 agtacatgta agcggtgtcg gtggcgaact ctgccgcaca ggtatccacg cgcttataaa
     9721 ccgggtgcag gtcatactgg tcgcgcagct tgcggatttc cgcttcgcac acgcccgcca
     9781 gttttgccag acgcgcatcg gcaaagcctt tgcgtttcag ctggcgcagg aattcagcgt
     9841 tcaggccagt gatgcccact tccgctactt tctcttccag acgcaccagt tcttcaatct
     9901 gtaccaggaa ccagcggtca atattggtca ggttgaagac gccgtccaca gacaggcccg
     9961 cacggaacgc atcggcgatg taccagatac gatctgcgcc tgcgtctttc agttcgcgac
    10021 ggattttggt taacgcttcc gggtcatcca ggctcacttt cgggtcgaat ccagtcgcac
    10081 cgacttccag gccgcgcagc gctttttgca gggattcctg ctgcgtgcga ccaatcgcca
    10141 tcacttcgcc aaccgatttc atctgagtgg tcagacggtc gttagcaccg gcgaattttt
    10201 cgaagttgaa gcgaggaatt ttagtaacca catagtcgat ggacggctcg aaggaggccg
    10261 gagtacgtcc gccagtgatg tcgttcatca gttcgtcgag ggtgtaaccc accgccagtt
    10321 tcgccgccac tttagcaatc gggaaaccgg tcgctttcga cgccagcgcc gaagaacggg
    10381 acacgcgtgg gttcatttcg ataacaatca gacgaccgtt tttcgggttc accgcaaact
    10441 gaacgttgga accaccggtt tcaacgccga tttcacgcag caccgccatc gaggcgttac
    10501 gcatgatttg atattctttg tcggtcagcg tttgggctgg cgcgacagtg atggagtcac
    10561 cggtgtggat gcccatcgca tcgaagtttt cgatagagca gacgatgatg cagttgtcgt
    10621 ttttatcacg caccacttcc atctcgtact ctttccagcc gatcagcgac tcatcaatca
    10681 gcaactcttt ggtcggagag agatccagac cgcgggcgca aatttcttca aactcttcac
    10741 ggttataagc gataccgccg ccgctaccgc ccatggtaaa ggatgggcga ataatgcacg
    10801 ggaagcccac gtcagcggca accgccagcg cttcttccat cgtgtgtgcg ataccggaac
    10861 gcgcggtttc cagaccaatt ttcttcatcg ctacgtcgaa acgacggcgg tcttctgctt
    10921 tatcaatcgc atcggcagtg gcaccaatca tggtgacacc gaactcttcc aacacgccct
    10981 gacgttccag ctccagcgcg cagttcagcg ccgtctgacc gcccatcgtt ggcagcaccg
    11041 cgtccgggcg ctctttttca ataatcttgc gtacaacttc ccagtgaatc ggctcgatgt
    11101 aggttgcatc agccatttcc gggtcggtca tgatggtcgc cgggttggag ttcaccagaa
    11161 tgacgcggta accctcttca cgcagggctt tacacgcttg cgcgccagag tagtcaaact
    11221 cacacgcctg accgataaca atcgggcccg cacccagaat caggatactt tttatatctg
    11281 tacgttttgg catggctctt ttactcctga ttacttagcg gttttacggt actgctcaat
    11341 taactcgata aagtggtcga acaacggcgc ggcgtcgtgt ggaccagggc tggcttcagg
    11401 gtgcccctgg aagctgaatg ccggtttatc ggtgcgatga atgccctgta acgtaccgtc
    11461 gaacagggat ttatgcgtga cacgcaggtt tgcaggtaat gttgcttcgt ccaccgcaaa
    11521 accgtggttc tgggcggtga tcattaccac gtttttctcc acatctttaa ccggatggtt
    11581 gccgccgtgg tgaccaaatt tcattttgac agtcttcgca ccgctcgcca gcgccagcag
    11641 ctgatgaccg agacagatgc cgaataccgg aatatcggtt tcgaggaatt tctggatggc
    11701 ggtaatggcg taatcgcacg gggccgggtc gccaggaccg ttggagagga agatgccgtc
    11761 tggattcatt ttcagcacat cttccgcaga agtttgcgcc ggaacgatgg tcaggcgaca
    11821 gcctctatcc accagcatcc gcaggatgtt gcgcttggca ccaaaatcat aagccacgac
    11881 gtggaacggc agctcgtctt cttttttcgc ttctggcagg ccaccggtca acgtccagct
    11941 cccttgtgtc cagctatagg cttctgcggt ggtcacttct tttgccagat ccatgccatt
    12001 cagacctggg aacgcgcggg ctttttctaa cgccagcgcc gcatccgggt tatcgcccgc
    12061 gataatgcag ccattctgtg cgcctttctc gcgcagtaaa cgcgtcagct tacgggtatc
    12121 gatatcggca atcgccacga tgttatggcg tttcaggtaa gaagagaggt cttcggtatt
    12181 acggaagttg ctggcaatca gcggcaggtc gcgaatcacc agaccttgtg catgtacctg
    12241 agaagattct tcatcggcgt cattggtgcc gacattgcca atatggggat aagtaagagt
    12301 aacgatttga cgagaatagg aaggatcagt gaggatttct tgataaccgg tcattgaagt
    12361 attgaaaacg acttccccaa ccgccgaacc tgttgcccct atggcccgac cgtgaaactg
    12421 ggttccgtct tccagaacca atagcgctga cttaatcaaa acaccctcca gagaatattc
    12481 actcacttta tttgcatatt aattcacaat gatgacgtaa atcaatgcaa atctgcttac
    12541 cgacgaattt ctggcaaacg gcggcattct ggagatatgg gcgctaaaag tcaacttaat
    12601 ggcgggattt tttatattat tttgtggctc atgcatgttt tacaggcatt aagcgacaaa
    12661 aagatcatct aacctgttct ggaaaacgct tgcgcagcaa gaagattgca tgaaataaaa
    12721 acgagcagaa aaaagtggac caaatggtca aaatttacat taaaacaaca aaaacagaag
    12781 acaaaacatg
//
LOCUS       DAPHBJ010000130        12789 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.131, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000130 DAPHBJ010000000
VERSION     DAPHBJ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.131"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(421..1512)
                     /gene="ychF"
                     /locus_tag="RHZ76_003189"
     CDS             complement(421..1512)
                     /gene="ychF"
                     /locus_tag="RHZ76_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDV2813781.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1629..2213)
                     /gene="pth"
                     /locus_tag="RHZ76_003190"
     CDS             complement(1629..2213)
                     /gene="pth"
                     /locus_tag="RHZ76_003190"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDV2813782.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2491..2769
                     /gene="ychH"
                     /locus_tag="RHZ76_003191"
     CDS             2491..2769
                     /gene="ychH"
                     /locus_tag="RHZ76_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HDV2813783.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2824..4503)
                     /gene="dauA"
                     /locus_tag="RHZ76_003192"
     CDS             complement(2824..4503)
                     /gene="dauA"
                     /locus_tag="RHZ76_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HDV2813784.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4628..5575)
                     /gene="prs"
                     /locus_tag="RHZ76_003193"
     CDS             complement(4628..5575)
                     /gene="prs"
                     /locus_tag="RHZ76_003193"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDV2813785.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5726..6577)
                     /gene="ispE"
                     /locus_tag="RHZ76_003194"
     CDS             complement(5726..6577)
                     /gene="ispE"
                     /locus_tag="RHZ76_003194"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDV2813786.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGADISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6577..7200)
                     /gene="lolB"
                     /locus_tag="RHZ76_003195"
     CDS             complement(6577..7200)
                     /gene="lolB"
                     /locus_tag="RHZ76_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HDV2813787.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7414..8670
                     /gene="hemA"
                     /locus_tag="RHZ76_003196"
     CDS             7414..8670
                     /gene="hemA"
                     /locus_tag="RHZ76_003196"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDV2813788.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8712..9794
                     /gene="prfA"
                     /locus_tag="RHZ76_003197"
     CDS             8712..9794
                     /gene="prfA"
                     /locus_tag="RHZ76_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDV2813789.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9794..10627
                     /gene="prmC"
                     /locus_tag="RHZ76_003198"
     CDS             9794..10627
                     /gene="prmC"
                     /locus_tag="RHZ76_003198"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDV2813790.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10624..11016
                     /locus_tag="RHZ76_003199"
     CDS             10624..11016
                     /locus_tag="RHZ76_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HDV2813791.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11020..11829
                     /locus_tag="RHZ76_003200"
     CDS             11020..11829
                     /locus_tag="RHZ76_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HDV2813792.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11865..12719
                     /gene="kdsA"
                     /locus_tag="RHZ76_003201"
     CDS             11865..12719
                     /gene="kdsA"
                     /locus_tag="RHZ76_003201"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HDV2813793.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3209 a         3215 c         3301 g         3064 t
ORIGIN      
        1 atctgcataa caaaaacaat atgcgccatt tttgcattac taataagaaa aagcaaaaaa
       61 catcgatatt catttttggt caatgggttt tctggcctat cgttgttatt gtgctactgg
      121 ttatcatttt catcttgcca gcatattgga gcgtgatcaa ttttgatcag ctgtgaaggg
      181 ggctgatgtg tagtgataca aaaacttacg ttatatcact gctaatgaga tatccgtcat
      241 ctgaacactt atgtctttaa ttattctcgt ggttcactat aggcaataag cacaaaagtg
      301 taggatgtta caagaatgat taggactcgg tgaaatgaaa aatccacgca attgcgtgga
      361 ttttatatac ttttgcgctc ttcatgagat ttagcgaaac ctcatgagac aataaattaa
      421 ttagacgttg aaaaggaagt tcatcacatc gccatctttc acgatgtaat ctttaccttc
      481 tgcacgcatt ttgcctgctt ctttcgcgcc ttgttcacct ttgtaagtga tgaaatcttc
      541 aaacgagatg gtttgtgcac ggataaagcc tttttcaaaa tcagtatgga ttttgcccgc
      601 tgcctgcggc gcggttgctc caaccggaat ggtccatgca cgcacttctt tcaccccagc
      661 ggtgaagtaa gtttgcaggt tcagcagttt ataaccggca cggatcacac ggttcaggcc
      721 cggctcttcc agcccaagct cctgcataaa ctcgtcacgt tcttcgtcgt ccagttcggc
      781 aatgtctgct tcaacagcag cacaaaccgg aaccacaaca gaaccttctt tcgccgcgat
      841 ttcacgcacc tggtcaagat atgggttgtt ttcaaaaccg tcttcgttga cgttggcgat
      901 gtacattgtt ggtttcagcg tcaggaagct caggtaacga atagccgctt tctcttcagc
      961 gcttaaatcc agcgcgcgca gcatacctgc attttccaac tggggcaggc atttttccag
     1021 gaccgccagc tcagctttcg cgtctttatc gccacctttg gctttcttct gtacgcgatg
     1081 aatcgcacgt tcgcaggtgt cgaggtctgc cagcgccagt tcggtgttga taacttcaat
     1141 atcgtcagcc gggttaactt tgccggaaac gtgaatgatg ttgtcatttt caaagcagcg
     1201 aacaacgtga ccgatcgctt cggtttcacg gatgttggtc aggaactggt tacccagacc
     1261 ttcgcctttc gatgcgcctt ttaccagacc ggcgatatcg acaaattcca tggtcgtggg
     1321 aagcgtacgc tgcggtttta cgatttcagc cagttgatcc aggcgaggat caggcattgg
     1381 tacgacgcct gtgttcggct caatggtgca gaatggaaag ttggccgctt caataccggc
     1441 tttggtcagc gcgttgaaca gggtagattt cccgacgttg ggcaaaccga cgataccgca
     1501 tttgaatccc atgatttaaa tcaccttaat atcttaataa tcaacctgtt attgctaaca
     1561 gattgcagaa atggaaataa ctttgcctat tatacacggc actcggcaaa aatgccgcag
     1621 acaacgactt attgcgcttt aaaggcgtgc aatcggttcg ttgctttggt caagccatct
     1681 gtaaaccaca tttcagtaca acgcgccgct tcgtcaatgg cttcatcaat taacttctgt
     1741 tcactaacag gcggtttgcc taacacaaaa ccgacaactt tatttttatc gcccggatga
     1801 ccgattccga tgcgtaaacg gtgaaagtta gggttattac ccaatttact gatgatgtct
     1861 ttcagtccat tgtgaccacc atggccaccg cctaatttaa atttggcgac gccaggaggc
     1921 agatccagtt cgtcgtgggc caccagaatt tcgtccggat taatgcggaa aaaactggcc
     1981 atcgccgcaa cggctttgcc gctgagattc ataaatgtag tcgggactaa caggcggaca
     2041 tcttcgcctc caagagtgac tcgcgaagta taaccaaaga atttagcctc ttcgcgcagc
     2101 ggagcgcgca aacgctctgc cagtaagtca acgaaccagg caccagcatt atgtcgcgtt
     2161 gcggcgtatt cagcaccggg gttcgccagg ccgacaatca atttaatcgt cacgtttttt
     2221 tgtcctgagt gtgtacataa ctggcgcgta gtttactggt tgcggccccg cttgacaaaa
     2281 aactgcgtat caaatgcaga taacgtaata attgcctgag tggactatta gaaagtcaag
     2341 gtgttcaggc gtttatttgt aaagttttgt tgaaataagg gttgtaattg tgatcacgcc
     2401 cgcacataac ccactgggtg ttgtctatac tttacacata aggaagaggg gtattccctg
     2461 ttacaaccca gaaagttccg gaggtgacat atgaaacgca aaaacgcttc gttactcggt
     2521 aacgtgctca tggggttggg tctggtggta atggtggtcg gcgtggggta ttcaatcctc
     2581 aaccagttac cacagtttaa tatgccccag tatttcgcac atggtgcagt gctaagtatt
     2641 ttcgtcggtg ccattctctg gctggcgggt gcccgtgttg gcgggcatga acaggtgtgc
     2701 gaccgttact ggtgggttcg tcactatgac aaacgttgcc gccgtagcga taatcgccgt
     2761 catagctaac aaataatgca gtttgctgac cagtcagttt gcgctgactg gtcaatctcg
     2821 tacttataaa tccgccatcg ccgcgcgacg attcgggaag aacgcgaggc gtcccgggat
     2881 cggttgaatg cctgcgcgag ccatagtgcg cagtggctgg aattccacgt tgcacacgcg
     2941 cagttcacat ccttcgggca gacgcttcac aaaacgctgg aacgcatcaa gaccaccagc
     3001 atcaagtacc ggaacggcat cccacttcag aatcacaatc cgtttgcctt caagacgtga
     3061 ctccaggtcc gtgaacaagc cttcagcagc agcaaaaaac agcgggccaa taacgcgcag
     3121 taccagaaca tcgtctggaa catctacgac taccggtgcc aggcgagtca tacgtgcgat
     3181 acgacgcata aacagcagcg atgccagcac gatccccacg ctgatggcaa taaccatatc
     3241 aaacagcacg gtcagcgaca tgcacagcag catgacaatg atgtcatctt tcggtgcatg
     3301 acgcagcaag tcgaccactt tatgcgcttc actcatgttc cacgccacca tcaacagcag
     3361 ggctgccata gcggaaagcg gcagccagga gagcagcggt gccagtacca gcagggcaag
     3421 aataaccaga atagagtgga tcaccgccga gataggggaa gttgccccgg cacggacgtt
     3481 agcggcagaa cgcgcgatgg cagctgtagc ggtaatacca ccaaagaacg gagcgataat
     3541 attccccagt ccctgtccaa ccagttcgct gttcgccttg tgtttcgtcc cggtcatacc
     3601 atccagtacc acggcgcaga gcagagattc gattgcgccg agcattgcca ttgagaatgc
     3661 cgcaggcagc agtgtgcgaa tagaatccca ggttagcgtg aattctgaat taggcagatc
     3721 ccacggcagc accagttgcg gcagcagttg cggaataccg ttaccctgag aaccatcggc
     3781 caggacgtag tggaattgcg aaccgatggt agcaacatgt ccgccgagca ggttaacaat
     3841 ccccatcacc gcgcaaccag ccagcaaggc cggaaggtga ccgggtaaac gaatgcccag
     3901 acgcggccaa aaaacaagaa tacctagcgt cacaatgcca atggcagcat cacccacatt
     3961 aatggtcggc agcgccataa ataatgcgcc gactttttgt agataatgtt ccgggacatg
     4021 ggccatttgc agaccgagaa aatctttaat ctgcatggta ccgatggtga tcccgatacc
     4081 cgaggtgaaa cctaaggtga cggaaaccgg aatatactca atcaggcgac caaagcgtgc
     4141 cagacccatc agaatcaaaa agatccccga cagcaaggtc gcaaccagca gtcctgccag
     4201 tccaaactgt tgcgacacgg gatagagaat taccacaaat gccgcagtcg gaccggaaac
     4261 gctaaagcgt gacccacccg tcagagcaat gacaatcccc gcaacagctg cggtatataa
     4321 accgtactgg ggtgccacac cactaccaat agccaacgcc atcgccagcg ggatagcaat
     4381 aatcccgacg gttatcccgg caatcaggtc acgggtaaac cgtgcggcag tatatttttc
     4441 tttccagcaa gcgtcgatca gagcgcggaa aggcatcaca tgtgaggaaa atattttgtt
     4501 cacaataatg tttcatccgt gagcgcatca tctgtcaact aaatggcagg tgaaggaggc
     4561 ataggtcata caaatggata ttacagacaa aaaaacccgc cgcagcgggt ctttaagccg
     4621 ggttcgatta gtgttcgaac atggcagaga tcgattcttc gttgctgata cgacgaatcg
     4681 cttcggccag catacctgac agggtcagag tacgcacgtt cggcagtgat ttgatttcat
     4741 cgctcagcgg aatggtatcg cagacaacga cttcatcaat tacagagtta cgcaggttgt
     4801 tcgccgcgtt gccagagaag atcgggtgag tcgcgtacgc aaatacacgt ttagcaccac
     4861 gttctttcag agcttcagca gctttacaca gcgtaccgcc agtgtcgatc atatcatcga
     4921 ccagtacgca gtcacgacct gcaacgtcac cgatgatatg catcacctgt gaaacgttcg
     4981 cacgcggacg acgtttgtcg atgattgcca tatcggtatc gttcagcagc ttagcgatag
     5041 cgcgggcacg cacaacgccg ccgatgtccg gagaaaccac aattgggtta tccagattca
     5101 gctgcagcat gtcttccagc aggatcgggc taccaaatac gttatcaacc ggaacgtcga
     5161 agaaaccctg aatctgttca gcgtgcagat ccactgtcag cacacggtca acaccgacgc
     5221 tggagaggaa gtctgcaacc actttcgcag tgattggtac acgagcggaa cggacgcgac
     5281 ggtcctggcg cgcatagcca aagtagggga taacagcggt gatacgacct gcggaagcac
     5341 gacgcagggc atcaaccata acgactaatt ccatcaggtt gtcgttagta ggggcacaag
     5401 tggactggat gatgaaaata tcaccaccgc gtacattttc attaatttgt acgctgactt
     5461 cgccatcgct aaagcgacct acagcggcgt cgccgagtga agtgtacagg cggttggcaa
     5521 tacgttgtgc tagttccggg gtggcgttac cagcaaaaag cttcatatca ggcacgagaa
     5581 gaacctcagg catgcgtcca ttggtggaaa gaatctgccg aaaactgtgc gggccaggca
     5641 tgatcctttc caggcggtgt attaaagagc gcgatgcaac gtctggaaca aggtgacgtt
     5701 gtcaccgaaa ctcagcttgc ccggcttaaa gcatggctct gtgcaatggg gaaagattaa
     5761 cgcctttcgc cacaaagcca ttgagccatt ccggggcttg ctctagcacc tggcgggctt
     5821 cagactctgt atcaaattca gcaaagacac aggcccctgt cccagtcagg cgcgacgggg
     5881 cgtattctaa cagccaggaa agcaccgcat caacctcgcg aaaacgtttt cttgcgataa
     5941 cctcgcaatc attgctgaat tcacatttta gcaacgtttc tattgacctt tttggcgtat
     6001 tgcgcgggag ttcaggatct ttaaaaatca ccggagtcgg aatacttaca ccagggtgcg
     6061 ccaccagata ccacttctct ggcggatcca ccggcgttag tatttcacca acgccttcgg
     6121 caaacgcggc atgcccccga acaaagacag gaacatctgc gcccagcgtc agccccattt
     6181 ccgccagctc atccatgctt agcccgcatt gccagagatg atttaatgcc accaggaccg
     6241 tcgcggcatt ggatgaacca ccgccgagac cgccgcccat cggcaaacgc ttatcaatgc
     6301 tgatatccgc accgcttccc gtcggaagac gcccgctgtc tgccgcagtt ttcatcaaca
     6361 accgcgctgc gcgaacgatc aggttatctt catgttccac gccttcaacg ggcgttaaca
     6421 gacgaatatc cccatcgtca cgaagctcaa tgctgatggt gtcgccgtaa tcaagaaact
     6481 gaaacagcgt ttgcagcgtg tggtaaccat ccgcacgctg accggtaatg tataaaaaca
     6541 gattaagttt tgccggagag ggccactgtg tccgcattat ttcactatcc agttatccat
     6601 ttttaacttg atgcgttgac caccgtcggt gagttccata ttggctggca tcgcaggttg
     6661 cgttttggtg tcataaccac cataaacaac cttccagttt ttgccattct ggctgtaggt
     6721 aatttcgctc aggcggtact ggtcgtccag tttgtagtcg gttgcatcac ccggtaaacc
     6781 taaaatccac tggcgcaagc tgttgagcgg aattggcatt ccggtcaatt tgccaatcat
     6841 ctcttcggcg tcatcggcgg tataacgctg acctttattg tcgactaact gcacgttacc
     6901 cggttgagca ttcagctcca gttccgtgct gcccaatggg ttagtgagca gcagacggta
     6961 gcgatcctgg ccggtttgct gccagaaaaa gcgggcgtac actttttgtt ggtcagaaat
     7021 ataagcgaac gcgccgcgag tctgatactg attaagattg cgcacgtctt gctgatgctg
     7081 acgccattgt ggcgaatccg ggcttttgcc aggacctttg ggcgtggtaa cggaacaggc
     7141 agtgagcaca agagcagcca gcggtagcag gcggataaga cgaaaatcgg gcaggggcat
     7201 agtgatgaca agtccttgag atacgttgca gttataaccc ttaatgctag cgttaccgtc
     7261 cgctatcgtc tatgttcaag ttgtcttaat tgccagaatc taacggcttt cggcaattac
     7321 tccaaaaggg ggcgctctct tttattgatc ttacgcatcc tgtatgatgc aagcagacta
     7381 accctatcaa cgttggtatt atttcccgca gacatgaccc ttttagcact cggtatcaac
     7441 cataaaacgg cacctgtatc gctgcgagaa cgtgtatcgt tttcgccgga taagctcgat
     7501 caggcgcttg acagcctgct tgcgcagccg atggtgcagg gcggcgtggt gctgtcgacg
     7561 tgcaaccgca cggaacttta tcttagcgtt gaagagcagg ataacctgca agaggcgtta
     7621 atccgctggc tttgcgatta tcacaatctt aatgaagaag atctgcgtaa aagcctctac
     7681 tggcatcagg ataacgacgc ggttagccat ttaatgcgtg ttgccagcgg cctggattca
     7741 ttggttcttg gggagccgca gatcctcggt caggttaaaa aagcgtttgc cgattcgcaa
     7801 aaaggccata tgaaggccag cgaactggaa cgcatgttcc agaaatcttt ctctgtagcg
     7861 aaacgcgttc gcactgaaac agatatcggt gccagcgctg tgtctgtcgc ttttgcggct
     7921 tgtacgctgg cgcggcagat ctttgaatcg ctctctacgg tcacagtgtt gctggtaggc
     7981 gcgggcgaaa ccatcgagct ggtagcgcgt catctgcgcg aacataaagt acagaagatg
     8041 attatcgcca accgcactcg cgaacgtgcc caaatactgg cagatgaagt tggcgcggaa
     8101 gtgattgccc tgagtgagat cgacgaacgt ctgcgcgaag ccgatatcat catcagttcc
     8161 accgccagcc cgttaccgat tatcgggaaa ggcatggtgg agcgcgcatt aaaaagccgt
     8221 cgcaaccaac caatgctgtt ggtggatatt gccgttccgc gcgatgttga gccggaagtt
     8281 ggcaaactgg cgaatgctta tctttatagc gtggacgatc tgcaaagcat catttcgcac
     8341 aacctggcgc agcgtaaagc cgcagcggtt gaggcggaaa ctattgtcgc tcaggaaacc
     8401 agcgaattta tggcgtggct gcgagcacaa agcgccagcg aaaccattcg cgagtatcgc
     8461 agccaggcag agcaagttcg cgatgagtta accgccaaag cgttagcggc ccttgagcag
     8521 ggcggcgacg cgcaagccat tatgcaggat ctggcatgga aactgactaa ccgcttgatc
     8581 catgcgccaa cgaaatcact tcaacaggcc gcccgtgacg gggataacga acgcctgaat
     8641 attctgcgcg acagcctcgg gctggagtag cagtacatca ttttcttttt ttacagggtg
     8701 catttacgcc tatgaagcct tctatcgttg ccaaactgga agccctgcat gaacgccatg
     8761 aagaagttca ggcgttgctg ggtgacgcgc aaactatcgc cgaccaggaa cgttttcgcg
     8821 cattatcacg cgaatatgcg cagttaagtg atgtttcgcg ctgttttacc gactggcaac
     8881 aggttcagga agatatcgaa accgcacaga tgatgctcga tgatcctgaa atgcgtgaga
     8941 tggcgcagga tgaactgcgc gaagctaaag aaaaaagcga gcaactggaa cagcaattac
     9001 aggttctgtt actgccaaaa gatcctgatg acgaacgtaa cgccttcctc gaagtccgag
     9061 ccggaaccgg cggcgacgaa gcggcgctgt tcgcgggcga tctgttccgt atgtacagcc
     9121 gttatgccga agcccgccgc tggcgggtag aaatcatgag cgccagcgag ggtgaacatg
     9181 gtggttataa agagatcatc gccaaaatta gcggtgatgg tgtgtatggt cgtctgaaat
     9241 ttgaatccgg cggtcatcgc gtgcaacgtg ttcctgctac ggaatcgcag ggtcgtattc
     9301 atacttctgc ttgtaccgtt gcggtaatgc cagaactgcc tgacgcagaa ctgccggaca
     9361 tcaacccagc agatttacgc attgatactt tccgctcgtc aggggcgggt ggtcagcacg
     9421 ttaacaccac cgattcggca attcgtatta ctcacttgcc gaccgggatt gttgttgaat
     9481 gtcaggacga acgttcacaa cataaaaaca aagctaaagc actttctgtt ctcggtgctc
     9541 gcatccacgc tgctgaaatg gcaaaacgcc aacaggccga agcgtctacc cgtcgtaacc
     9601 tgctggggag tggcgatcgc agcgaccgta accgtactta caacttcccg caggggcgcg
     9661 ttaccgatca ccgcatcaac ctgacgctct accgcctgga tgaagtgatg gaaggtaagc
     9721 tggatatgct gattgaaccg attatccagg aacatcaggc cgaccaactg gcggcgttgt
     9781 ccgagcagga ataatggaat atcaacactg gttacgtgaa gcaataagcc aacttcaggc
     9841 gagcgaaagc ccgcggcgtg atgctgaaat cctgctggag catgttaccg gcagagggcg
     9901 tacttttatt ctcgcctttg gtgaaacgca gctgactgac gaacaatgtc agcaacttga
     9961 tgcgctactg acacgtcgtc gcgatggtga acccattgct catttaaccg gggtgcgaga
    10021 attctggtcg ttgccgttat ttgtttcgcc agcgacctta attccgcgcc cggatacgga
    10081 gtgtctggtg gagcaggcac tggcgcggtt gcctgaacaa ccttgccgta ttctcgatct
    10141 cgggacgggt accggggcga ttgcgcttgc gctggctagc gagcgcccgg actgcgaaat
    10201 tatcgctgta gatcgtatgc ctgatgctgt ctccctggca caacgtaatg cccagcatct
    10261 ggcgatcaaa aatatccaca ttctgcaaag cgactggttt agcgcgctag ccgggcagca
    10321 gtttgcgatg attgtcagca atccgccgta tattgacgag caggaccctc atcttcaaca
    10381 aggcgatgtc cgctttgagc cgctcactgc gctggttgcg gcagacagtg gaatggcaga
    10441 catcgtgcat atcatcgaac agtcgcgtaa cgcgctggta tccggcggct ttctgcttct
    10501 ggaacatggc tggcagcagg gcgaagcggt gcgacaagca tttatcctcg cggggtatca
    10561 tgacgtcgaa acctgccgtg actatggtga taacgagcgc gtaacgctcg gccgctatta
    10621 tcaatgacaa gtttttctac actgcttagt gttcatctta ttagtatcgc gctttctgtt
    10681 gggctattaa ccttacgttt ctggctacgt tatcagaagc atccacaggc atttgctcgc
    10741 tggacgcgca ttgtgccgcc ggttgtcgat acgctgttac tgttaagcgg cattgcgttg
    10801 atggctaaag cgcacatcct gccattttcc gggcaggcac agtggctgac tgaaaagctg
    10861 tttggagtta tcatttatat cgttttgggt tttattgcac tcgattatcg tcgtatgcac
    10921 agtcagcagg cgcgcattat tgccttcccg ctggcgttgg tggtgctgta catcatcatt
    10981 aaactcgcca ccacaaaagt accgttactg gggtaagtca tgagatcgtt agctgatttc
    11041 gaatttaata aagcgccatt gtgcgaaggc atgatcctgg cttgcgaagc aatccgccgc
    11101 gattttccct cgcaagatgt ttacgacgaa ctggagcgtc tcgttagtct ggcgaaggaa
    11161 gaaatcagcc agcttctgcc tttagaagag cagttggaaa aactgatcgc gctgttttac
    11221 ggcgactggg gatttaaagc ctcacgcggt gtttatcgtc tttccgatgc attatggctg
    11281 gaccaggtgt taaagaatcg acagggcagt gcggtatcat taggtgcggt tttattatgg
    11341 gtcgcgaatc gtctcgattt gccgctgctg ccggtgattt tccctacgca gctgatattg
    11401 cgcattgaat gtccggatgg cgaaatttgg ctgattaatc cttttaacgg tgaatcgtta
    11461 agcgaacata tgctggacgt atggttaaag ggaaatatca gcccgtcggc ggaactgttt
    11521 tatgaagacc ttgatgaagc tgataacatt gaggtaatcc gcaaattgct ggatacactc
    11581 aaagcctcgt tgatggaaga aaatcagatg gagctggcgt tacgcaccag cgaagcttta
    11641 ttacaattca accctgaaga tccctatgaa attcgcgatc gcgggttgat ttatgcgcaa
    11701 ctggattgcg aacacgttgc gttgaacgat ttaagttatt tcgttgaaca gtgtccggaa
    11761 gacccgatca gcgaaatgat ccgtgcgcaa ataaataaca tcgcgcataa acatattgtg
    11821 ctgcattaat taatcgacat tttactcaag attaaggcga tcctatgaaa caaaaagtgg
    11881 ttagcattgg cgacatcaac gtagcaaatg acctgccgtt cgtactgttt ggcggtatga
    11941 acgtgttgga atctcgcgat ctggcgatgc gcatttgcga gcactacgta actgtgaccc
    12001 agaaactggg tatcccttac gtgttcaaag cctcttttga caaagccaac cgctcctcca
    12061 tccactctta tcgtggaccg ggcctggaag aagggatgaa aatcttccag gagttgaagc
    12121 agacttttgg cgtgaaaatt atcaccgacg ttcacgaacc aagtcaggca cagcccgttg
    12181 ctgatgtcgt ggatgtgatt cagttgccgg cgtttcttgc tcgccagact gacctggttg
    12241 aagccatggc gaaaaccggt gcggtaatta acgtcaagaa accacagttt gtcagcccgg
    12301 gacagatggg taatatcgtt gataaattca aagaaggcgg caacgaaaaa gtgattcttt
    12361 gcgatcgcgg tgctaacttc ggctatgaca acctggttgt cgatatgctg ggcttcagca
    12421 ttatgaagaa agtgtctggt aactcgccgg tgattttcga cgtgacccac gcactgcaat
    12481 gccgcgatcc gtttggcgca gcttccggtg gtcgtcgtgc tcaggtggct gagctggcac
    12541 gagccggtat ggcggtaggt ctggcggggc tgtttattga agcgcatccg gatccggaac
    12601 atgcgaaatg tgatggtcca tccgcgctgc cgctggctaa actggaaccg ttcctcaagc
    12661 agatgaaagc gattgatgat ctggtgaaag gtttcgaaga actggatacc agcaagtaat
    12721 ctttttgttt gaaaaataaa gtcttagcgt tgtgcgttaa gactttttta tgggtgccgg
    12781 atacaaaaa
//
LOCUS       DAPHBJ010000131        12688 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.132, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000131 DAPHBJ010000000
VERSION     DAPHBJ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.132"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(65..1423)
                     /gene="plaP"
                     /locus_tag="RHZ76_003202"
     CDS             complement(65..1423)
                     /gene="plaP"
                     /locus_tag="RHZ76_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HDV2813794.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1413..1475)
                     /gene="yoeI"
                     /locus_tag="RHZ76_003203"
     CDS             complement(1413..1475)
                     /gene="yoeI"
                     /locus_tag="RHZ76_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HDV2813795.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1690..2619)
                     /locus_tag="RHZ76_003204"
     CDS             complement(1690..2619)
                     /locus_tag="RHZ76_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2813796.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2665..3489)
                     /locus_tag="RHZ76_003205"
     CDS             complement(2665..3489)
                     /locus_tag="RHZ76_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV2813797.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGSGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3572..3826)
                     /gene="yoeB"
                     /locus_tag="RHZ76_003206"
     CDS             complement(3572..3826)
                     /gene="yoeB"
                     /locus_tag="RHZ76_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HDV2813798.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(3823..4074)
                     /gene="yefM"
                     /locus_tag="RHZ76_003207"
     CDS             complement(3823..4074)
                     /gene="yefM"
                     /locus_tag="RHZ76_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HDV2813799.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            4358..4408
                     /gene="hisL"
                     /locus_tag="RHZ76_003208"
     CDS             4358..4408
                     /gene="hisL"
                     /locus_tag="RHZ76_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HDV2813800.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    4384..4507
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4554..5453
                     /gene="hisG"
                     /locus_tag="RHZ76_003209"
     CDS             4554..5453
                     /gene="hisG"
                     /locus_tag="RHZ76_003209"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDV2813801.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5459..6763
                     /gene="hisD"
                     /locus_tag="RHZ76_003210"
     CDS             5459..6763
                     /gene="hisD"
                     /locus_tag="RHZ76_003210"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDV2813802.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGNEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVRDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALSASRLIVTNDLAQCVTISNQYGPEHLIIQTRN
                     ARELVDSITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6760..7830
                     /gene="hisC"
                     /locus_tag="RHZ76_003211"
     CDS             6760..7830
                     /gene="hisC"
                     /locus_tag="RHZ76_003211"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDV2813803.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEDVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEISCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7830..8897
                     /gene="hisB"
                     /locus_tag="RHZ76_003212"
     CDS             7830..8897
                     /gene="hisB"
                     /locus_tag="RHZ76_003212"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HDV2813804.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8897..9487
                     /gene="hisH"
                     /locus_tag="RHZ76_003213"
     CDS             8897..9487
                     /gene="hisH"
                     /locus_tag="RHZ76_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDV2813805.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLARRSEESNGVDLLGIIDED
                     VPKMTDFDLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9487..10224
                     /gene="hisA"
                     /locus_tag="RHZ76_003214"
     CDS             9487..10224
                     /gene="hisA"
                     /locus_tag="RHZ76_003214"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDV2813806.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLIAGVNVPVQVGGGVRSEEDVAALLEAGVVRVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAVLRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10206..10982
                     /gene="hisF"
                     /locus_tag="RHZ76_003215"
     CDS             10206..10982
                     /gene="hisF"
                     /locus_tag="RHZ76_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDV2813807.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            10976..11587
                     /locus_tag="RHZ76_003216"
     CDS             10976..11587
                     /locus_tag="RHZ76_003216"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDV2813808.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGNVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11682..12662)
                     /gene="wzzB"
                     /locus_tag="RHZ76_003217"
     CDS             complement(11682..12662)
                     /gene="wzzB"
                     /locus_tag="RHZ76_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HDV2813809.1"
                     /translation="MRVENNNVSGQNLDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQPLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     VELEKDLKDNIALRMKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTGEDITQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIH
                     AYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLRRNALRNYNAK"
BASE COUNT         3142 a         3351 c         3279 g         2916 t
ORIGIN      
        1 aaaaagaata aaaaaaccgg agccaaatgt tcattcgact ccggtttatt attagaaaag
       61 atgattatgc tacgtcttcg tactgcggaa ccggattacg gaaactcttg gttacgcaag
      121 ccaggtaaat cagaccgata gcagcccaga tcagacccag aaccattgag ctttcttcca
      181 ggttaaccca cagcgcacca acggtcagcg caccacacat cggcaggaac agatactgga
      241 agtgatcttt cagcgtcttg ttacgcttct cacggatcca gaactgcgaa ataaccgaca
      301 ggttaacgaa ggtgaacgcc accagcgcac caaagttaat cagcgccgta gccattacta
      361 ggtcgaagtt gattgccagc aaggcaatcg cgccaaccag gatgatgttc attgccggag
      421 tacgccattt cgggtgtaca taaccgaaga agcttttcgg gaacacaccg tcacgcccca
      481 ttacgtacat cagacgcgct acgcctgcat gcgccgccat accggacgcc agtacggtaa
      541 tggtggagaa gatcagcgcg ccaacctgga acgctttacc cgcaacgtac agcatgattt
      601 caggctgtga cgcatccgga tctttaaagc gagagatatc cgggaagtac agctgcagga
      661 agtaagttgc aaagatgaag atcatgccgc caatcagcgc ggtcaggaaa atcgcacgcg
      721 ggatcacgcg ctctgcatct ttggtttctt ccgacaggtt gctgatgccg tcaaagccgg
      781 taaaggagaa gcacaggatt gtcgccccgg taatcatcgg gataacatgc gcatcgccag
      841 accagaacgg acgagtgctc gccagcgtac ccgcgccttc accttcaaat acgccataaa
      901 caaccatgcc cagaatcacc gcgatcagca ctacctgcaa cacgacgatt acagtgttga
      961 agttcgctac ggatttcaga ctacgcaggt taaaggcggt cataaaggcc accagcgcca
     1021 ccacaaacat ccacgatggg atggaaggca ccagagcttc aaaatagatt ttcgccagca
     1081 gaatgttgat catcggcgcg aacagatagt cgagcagaga agaccaaccc accataaagc
     1141 cgacagtcgg gctaatggat ttctgggcgt aagtgtatgc agagccagca gaaggatagc
     1201 ggcgaaccag cttcccgtag ctcagagccg taaacaggat cgcaatcaat gcgaacgcat
     1261 aggctgtcgg cacatgacca tccgtgaggc ctgaaacgat accaaatgta tcaaacagcg
     1321 tcatcggctg catataggca agacccatca ttacaaccgg aactaacgta agcgttttac
     1381 gcaattccac gcgagaggtg tttggagtaa cgttatgcga catggtcatt ctcctttacg
     1441 gtgataaccg tcgcgtaagc aaaaaattgc cccatttttt tggattcctc agcgacaaca
     1501 actgtcgatt tttagtaaat atctatccgg tacgaagccc ggcctcttgg tatgaatgat
     1561 tggtttgaag caaaaaataa ccgacgctga tgaaacgtcg gtttttagtc attttttgac
     1621 agcggcgcat tgtgcctaaa tgggggggaa atgacaagag aatgagaggc ttgtcataat
     1681 aatttttctt taaatggctg attttccgtc atcagatttt gcataaacac cggcgatcgc
     1741 actatttgct aaaatttcat ctcgccacca gccgctggca agccctgcgg tctgttcgtt
     1801 ccagccaatc cagacgggtt caaagagagt ttgggcgact actttcttct cgattaacgc
     1861 gccactatcg agaaaacgtt gcgccagata acggggtaaa tagccgcagc caaggccgct
     1921 aatttgcagc tccagcttgg ttttaaaatc gaaaacggta atggcttctt gttcgtcgag
     1981 aagttgtgag gccgtagaag cgccggcctg tgcagtgtcc cccaccacaa tcgcccgata
     2041 tcgtttaatg atacgacggt ttaacggttc ttcttccagg gctaacggat gatgaggcgc
     2101 gaccgcaaag acttgttcca gatcgcccaa ccgcgagaaa ccaaactcac tggaagatgg
     2161 tggctcatgc atcgcgccaa caataatatc cgctcgcccc tgagtcaggg catcccagga
     2221 accaccgagt acgccattga taaacttcag gcgcgtcacg ctatgatgtt gataaaaagc
     2281 ttcaatcagt ggcgcaagca gagaaaaagg aaacgtgtcg tccacgccaa tcaccagctc
     2341 gttttcccag ccttcatgca gtttgatcgc ctgcttttcc agttcccgca cggtatgcag
     2401 aacttcccgc cctttctcta ataacatttt tccggtacgg gtgaatttag cgcggtgacc
     2461 gctacgatca agcaattgaa tattaaggtc gctttccagc ttgtgaacgg tgtaactcag
     2521 agcggacggc gtcttataga gtttggccga cgccgccgca aaactgcctt ctttttctaa
     2581 ggcatcgagg atcatcaata catccagcag tggtttcata cacgccccct tgcagtgtat
     2641 gcggcgtcct gctggcgtga tcgtttactc cagcggcatt accagcggat cgggatactg
     2701 gtattcaaac cccagttcat tacaaatccg actgccatca ataatcttac ctttgccgct
     2761 gtccagacta tttctgaact gcggaggttc cagccccagt aaacgggcca tctgcggata
     2821 gaaaacatta cgcgcagggt gagctggcgc acatatatta tagatgtgtc cgcctttagg
     2881 tgcctgtaac aacagagtga tagcgccaat aacatcttct aaatggacta aattaacacc
     2941 atgttcacca tcaggcgcgg tttttccggc aaagaagcgt ccgggatgac gtcccggtcc
     3001 gaccagcccc gcaagacgca gaatatcgac cgaagtaccg ggtaaattgt gcagccagtc
     3061 ttcgagttct tctaatactc gtccactgtt ggttaccgga ttacgcgggg tggtttcttt
     3121 caccgtgcct tgcgcgtcgc catagacaga tgttgagctg gtaaaaataa ttcggggaat
     3181 acgatgggcc agcgcgctat ccactaactc ttgtaccgct tgtaaataga actcatcgcc
     3241 ggagccgcta cgacgtgccg gaagcgtaat gaccagtgca tcggcatcca tcagggcatc
     3301 cagatcgtca gaatcgcaaa ctaactcagg ctccatgcga agcagatagc tatcaatgcc
     3361 actcattcgg gccgcttcga caccatcttg tgtggtttta ctcccggtga cttgccagcc
     3421 tcgcgctgaa agtgacatcg ccagcggcat gcctaaccac cctaacccga caattgcgac
     3481 ctttttcatc cgttatctcc tgatacaact tagatctata aggctacgct agcgtatcaa
     3541 aactgacaat tcattctatg aatgaatctg ttcaataatg ataacgacat gctgcaatga
     3601 gcagtgaatc gtcagtaacc gcgtatacca gacggtgctc ctctgtaatg cgtcgggacc
     3661 agaagcctga caaattatgc ttcaggggtt ctggcttccc cttaccttca aatggtgttc
     3721 tgcgggtatc tttgataagt tcattgatct ttttaacaat tcgcttatct gtttcctgcc
     3781 agtacagata atcatcccat gactcctcag accagattag tttcactcaa taatgtcctt
     3841 ttccgttcct ttgcctgatt tcaggctatc gattgagtcc atcaatctcc gggcgttagc
     3901 gggggaacgc agtagataag ccgtctcttc cagcgagttg tattcttcga gtgacatcag
     3961 aacacaagcc tctccattct gacgagtaat aaggatcggg gcatgatctt caacggcttt
     4021 catcattgtt gccgacaaat tctgacgcgc ttcgctgtaa ctaattgtac gcatgtcaat
     4081 ctcctctttt gtacagctta ttgtacaatg atagacgttg attcactatt tattaattag
     4141 tttgtagatc aaggcattgt cagtgagacg aaaaatccag gcttcgctat tttgggtacc
     4201 gtcaggttag aggacagtcc tcttagcgcc ctcctttccc cgctcattca ttaaacaaat
     4261 ccatcgccat aaaatatata aaaaagccct tgctttctaa cgtgaaagtg gtttaggtta
     4321 aaagacatca gttgaataaa cattcacaga gacttttatg acacgcgttc aatttaaaca
     4381 ccaccatcat caccatcatc ctgactagtc tttcaggcga tgtgtgctgg aagacattca
     4441 gatcttccag tggtgcatga acgcatgaga aagcccccgg aagatcacct tccgggggct
     4501 tttttattgc gcggttgata acggttcaga caggtttaaa gaggaataag aaaatgacag
     4561 acaacactcg tttacgcata gctatgcaga aatccggccg tttaagtgat gactcacgcg
     4621 aattgctggc gcgctgtggc attaaaatca atcttcacac ccagcgcctg atcgcgatgg
     4681 cagaaaacat gccgattgat attctgcgcg tgcgtgacga cgacattccc ggtctggtaa
     4741 tggacggcgt ggtagacctt ggaattatcg gcgaaaacgt gctggaagaa gaactgctta
     4801 accgccgcgc ccagggtgaa gatccgcgct actttaccct gcgtcgactg gatttcggcg
     4861 gctgccgcct ttcgctggca acgccggttg atgaagcctg ggacggcccg ctctccttaa
     4921 atggtaaacg tatcgccacc tcttatcctc acctgctcaa gcgttatctc gaccagaaag
     4981 gcatctcttt taaatcctgc ttactgaacg gttctgttga agtcgccccg cgtgccggac
     5041 tggcggatgc gatttgcgat ctggtttcca ccggtgccac gctggaagca aacggcctgc
     5101 gtgaagtcga agttatctac cgctcgaaag cctgcctgat ccagcgcgat ggcgaaatgg
     5161 aagaatccaa acagcaactg atcgacaaac tgctgacccg tattcagggt gtgatccagg
     5221 cgcgcgaatc aaaatacatc atgatgcacg caccgaccga acgtctggat gaagtcatcg
     5281 ccctgctgcc aggtgccgaa cgcccaacca ttctgccgct ggcgggtgac caacaacgcg
     5341 tagcgatgca catggtcagt agcgaaaccc tgttctggga aacgatggaa aaactgaaag
     5401 cgctgggtgc cagttcaatc ctggtcctgc cgattgagaa gatgatggag tgatcgccat
     5461 gagctttaac acaatcattg actggaatag ctgtactgcg gagcaacaac gccagctgtt
     5521 aatgcgcccg gcgatctccg cttctgaaag cattacccgc actgttaacg atattctcga
     5581 taacgtgaaa gcacgcggca atgaggccct gcgggaatac agcgcgaagt ttgataaaac
     5641 cacggttacc gcactgaagg tgtctgctga ggaaattgcc gccgccagcg aacgcctgag
     5701 cgacgagcta aaacaggcga tggcggtggc agtaaagaat attgaaacct tccacactgc
     5761 gcaaaaactg ccgccggtag atgtagaaac gcagccaggc gtgcgttgcc agcaggtcac
     5821 gcgcccggtc gcttcagttg ggttgtatat tcctggcggc tccgccccgc tcttctcaac
     5881 ggtattaatg ctggcaactc cggcgcgtat tgcgggctgt aaaaaagtgg tgctgtgctc
     5941 accgccgccg attgccgatg agatccttta tgcggcgcag ctgtgcggtg tgcgggacgt
     6001 gtttaacgtc ggcggcgcac aggccattgc cgcgctggcg tttggtacgg aatctgtgcc
     6061 gaaagtggac aaaatcttcg ggccgggtaa cgcctttgtc accgaagcaa aacgccaggt
     6121 aagccagcgt ctggacggtg cggcgatcga tatgcccgca ggcccgtcgg aagtgctggt
     6181 gattgctgac agcggcgcta cgccggattt tgtggcttct gatttgcttt cccaggctga
     6241 acacggcccg gactctcagg tgattttact gacgcccgat gctgatatgg cgcgtcgtgt
     6301 agccgaagct gtcgaacgcc aactggcaga actgccgcga gctgaaaccg cacgtcaggc
     6361 actgagcgcc agccgcctga ttgtgaccaa cgatttagcg cagtgcgtaa cgatttccaa
     6421 ccagtacgga ccggagcacc tgatcattca gacccgcaac gcccgtgaac tggtcgatag
     6481 catcaccagc gccggttcgg tatttcttgg tgactggtca ccggaatcgg caggtgatta
     6541 cgcctccggc accaaccacg ttctgccgac ttacggttac accgccacct gttccagcct
     6601 cgggctggca gatttccaga agcggatgac cgtgcaggaa ctgtcgaaag aaggtttctc
     6661 cgcgctggct tcgaccattg aaacactggc cgccgccgag cgcctgaccg cccacaaaaa
     6721 tgccgttact ttgcgtgtta acgcccttaa ggagcaagca tgagcaccgt gactattacc
     6781 gatttagcgc gtgaaaacgt ccgcaacctg acgccgtatc agtcagcgcg tcgtctgggc
     6841 ggtaacggcg atgtctggct gaacgccaac gaatacccca cagccgtgga gtttcagctc
     6901 actcaacaaa cgctcaaccg ctacccggaa tgtcagccaa aagcagtgat tgaaaattac
     6961 gcacaatatg caggcgtaaa accggagcag gtgttggtca gccgtggcgc ggacgaaggt
     7021 attgaactgc tgattcgcgc tttttgcgaa ccgggtaaag acgccatcct ctactgcccg
     7081 ccaacgtacg gcatgtacag cgtcagcgct gaaaccattg gcgtcgagtg ccgcacagtg
     7141 ccgacgctgg acaactggca actggactta cagggcattt ccgacaagct ggacggcgta
     7201 aaagtggtct atgtttgcag ccccaacaac ccgaccgggc aactgatcaa cccgcaggat
     7261 tttcgcactc tgctggagtt aacccgcggt aaagcgattg tggttgccga tgaagcctat
     7321 atcgagtttt gcccgcaggc atcgctggct ggctggctga cggaatatcc gcacttggct
     7381 attttgcgca cactgtcgaa agcttttgct ctggcgggcc ttcgttgcgg atttacgctg
     7441 gcaaacgaag atgtcatcaa ccttctgatg aaagtgatcg ccccctaccc gctctcgacg
     7501 ccggtcgccg acattgcggc ccaggcgtta agcccgcagg ggatcgtcgc catgcgcgaa
     7561 cgggtggcgc aaattattgc agaacgcgaa tacctgattg ccgcattgaa agaaatctcc
     7621 tgcgtggagc aggtttttga ctctgaaacc aactacattc tggcgcgctt taaagcctcc
     7681 agcgcagtgt ttaaatcttt gtgggatcag ggcattatct tacgtgatca gaataaacaa
     7741 ccctctttaa gcggctgcct gcgaattacc gtcggaaccc gtgaagaaag ccagcgcgtc
     7801 attgacgcct tacgtgcgga gcaagtttaa tgagtcagaa gtatcttttt atcgatcgcg
     7861 atggaaccct gattagcgaa ccgccgagtg attttcaggt ggaccgtttt gacaaactcg
     7921 cctttgaacc gggcgtgatc ccagagctgc tgaagctgca aaaagcgggc tacaaattgg
     7981 tgatgatcac caatcaggat ggtcttggaa cacaaagttt cccgcaggcg gattttgatg
     8041 gcccgcacaa cctgatgatg cagatcttca cctcgcaagg cgtgcagttt gatgaagtgc
     8101 tgatttgccc gcacctgccc gccgatgagt gcgactgccg taaaccgaaa gtaaaactgg
     8161 tggagcgtta tctcgctgag caagcgatgg atcgcgccaa cagttatgtg attggcgatc
     8221 gcgcgaccga tattcaactg gcggaaaaca tgggtattaa tggtttacgc tacgaccgcg
     8281 aaaccctgaa ctggccgatg attggcgagc aactcactaa acgagaccgt tacgcccatg
     8341 tagtgcgcaa caccaaagag acgcagattg acgttcaggt gtggctggat cgcgaaggcg
     8401 gcagcaagat taacaccggc gttggcttct ttgatcatat gctggatcag atcgctaccc
     8461 acggcggttt ccgtatggaa atcaacgtca aaggcgacct ctatatcgac gatcaccaca
     8521 ccgtcgaaga taccggcctg gcgctggggg aagcgttaaa aattgccctc ggcgataaac
     8581 gcggtatttg ccgctttggt tttgtgctgc cgatggacga atgccttgcc cgctgcgcgc
     8641 tggatatctc tggtcgcccg cacctggaat ataaagccga gtttacctac cagcgcgtgg
     8701 gcgatctcag caccgagatg atcgagcact tcttccgttc gctctcttac accatgggcg
     8761 tgacgttgca cctgaaaacc aaaggtaaaa acgatcatca ccgtgtagag agtctcttta
     8821 aagcctttgg tcgcaccctg cgccaggcca tccgcgtgga aggcgacacc ctgccctcgt
     8881 cgaaaggagt gctgtaatga acgtggtgat ccttgatacc ggctgcgcca atttgaactc
     8941 ggtgaagtca gccattgcgc gtcacggtta tgaacccaaa gtcagccgtg acccggacgt
     9001 cgtgttgctg gccgataaac tatttttacc cggcgtaggc accgcacaag cagcgatgga
     9061 tcaggtgcgt gagcgcgagc tgtttgatct catcaaagcc tgtacccaac cggtgctggg
     9121 catctgctta gggatgcaac tgctggcgcg gcgcagcgaa gagagcaacg gcgtcgattt
     9181 gctgggcatc atcgacgaag acgtgccgaa aatgaccgac tttgatctgc cgctgccgca
     9241 tatgggctgg aatcgcgttt acccgcaggc aggcaaccgc ctgtttcagg ggattgaaga
     9301 cggcgcgtac ttttactttg ttcacagtta cgcaatgccg gtcaatccgt ggaccatcgc
     9361 ccagtgtaat tacggcgaac cgttcaccgc ggcggtacaa aaagataact tctacggcgt
     9421 gcagttccac ccggagcgtt ctggtgccgc tggcgctaag ttgctgaaaa acttcctgga
     9481 gatgtaatga ttattccggc attagattta atcgacggca ccgtggtgcg tctccatcag
     9541 ggcgattacg gcaaacaacg cgattacggt aacgacccgc tgccgcgctt acaggattac
     9601 gccgcgcagg gtgccgaagt gctgcacctg gtggatctga ccggggcaaa agatccggct
     9661 aaacgtcaaa tcccgctgat taaaaccctg atcgcgggcg ttaacgttcc ggtgcaggtt
     9721 ggcggcggtg tgcgtagcga agaagacgtg gcggcgttac tggaagcagg cgttgtgcgt
     9781 gtggtggttg gctccaccgc ggtgaaatca ccagaaatgg tgaaaggctg gtttgaacgt
     9841 ttcggtgccg atgccttagt gctggcgctg gatgtccgta ttgacgagca aggcaacaag
     9901 caggtggcag tcagcggctg gcaagagaac tcgggcgttt cactggaaca actggtggaa
     9961 acctatctgc ccgtcggcct gaaacatgtg ctgtgtaccg atatctcgcg cgacggcacg
    10021 ctggcaggtt ctaacgtctc tttatatgaa gaagtgtgcg ccagatatcc gcaggtggca
    10081 tttcagtcct ccggcggcat tggcgacatt aacgatgttg ctgtcctgcg tggaactggc
    10141 gtgcgcggcg taattgtcgg tcgcgcatta ctggaaggta aattcaccgt gaaggaggcc
    10201 atcgcatgct ggcaaaacgc ataattccat gtctcgacgt tcgggatggt caggtggtga
    10261 aaggcgtaca gtttcgcaac catgaaatca ttggcgatat cgtaccgctg gcaaaacgct
    10321 acgccgaaga aggtgcagac gaactggtgt tctacgatat caccgcttcc agcgatggcc
    10381 gtgtggtaga taaaagctgg gtatcgcgcg tggcggaagt gatcgacatt ccgttctgcg
    10441 tggcgggtgg gattaagtct ctggaagatg ccgcgaaaat tctttccttt ggcgcggata
    10501 aaatttccat taactcccct gcgctggcgg acccgacgtt aattactcgc ctggcggatc
    10561 gctttggcgt gcaatgcatt gtggtcggca ttgatacctg gtacgacgct gaaaccggta
    10621 aatatcatgt gaatcaatat accggcgatg aaagccgcac ccgcgttact cagtgggaaa
    10681 cgctcgactg ggtagaggaa gtgcaaaaac gcggtgccgg agaaattgtc ctcaacatga
    10741 tgaatcagga cggcgtgcgt aacggttacg acctcgagca gctgaaaaaa gtgcgtgaag
    10801 tttgccacgt cccgctgatt gcctccggtg gcgcgggcac catggaacac ttccttgaag
    10861 ccttccgcga tgccgacgtt gacggcgcgc tggcagcttc cgtattccac aaacaaataa
    10921 tcaatattgg tgaattaaaa gcgtacctgg caacacaggg cgtggagatc aggatatgtt
    10981 aacagaacaa caacgtcgcg aactggactg ggaaaaaacc gacggactga tgccggtgat
    11041 tgtgcaacac gcggtatccg gcgaagtgct aatgctgggc tatatgaatc cggaagctct
    11101 ggacaaaacc atagaaagcg gcaatgtcac cttcttctcg cgcactaaac agcgactgtg
    11161 gaccaaaggc gaaacgtcgg gcaatttcct caatgtggtg agtattgccc cggactgcga
    11221 caacgacacg ttactggtgc tggcgaatcc catcgggcca acctgccaca aaggcaccag
    11281 cagctgcttc ggcgacaccg ctcaccagtg gctgttcctg tatcaactgg aacaactgct
    11341 cgccgagcgc aaatctgccg atccggaaac ctcctacacc gccaaactgt atgccagcgg
    11401 caccaaacgc attgcgcaga aagtgggcga agaaggcgtg gaaaccgcac tggcagcaac
    11461 ggtacatgac cgctttgaac tgaccaacga ggcatctgat ttgatgtatc acctgctggt
    11521 gctgttgcag gatcaggatc tggatttaac gacggtaatt gagaacctgc gtaaacggca
    11581 tcagtgagtt gcgggtgatc agatgcgatg ttgttgtcgc atccggcaaa aaaaatgggc
    11641 aaggtgtcac caccctgccc gttttcttta aaaccaaaag attacttcgc gttgtaatta
    11701 cgcagagcgt tacgcctcag cacaatcccc gcgccaacca tgccgcccag cagcactgcc
    11761 agaatcaagg taattgcctt tttcggacta tcgcgacgaa taggtaacgt cggtttcatc
    11821 acataacggt aagcatgaat atcaagatca tcaaccttta agctttcgat atccagcagg
    11881 ttttgacgag tctgatagta gtttggtgag aacaccaacg gacgggtcgc ctcgtgctta
    11941 atcatcgact ccagcgcttc gctccccaaa aggaacaacg tatcttgtgt gatatcttcg
    12001 ccagtctgtt gaatctgcgg ttttgtcacc tgcgcctgat tcgcatactg caacgcttcc
    12061 tgaatctgac ggatacgcag atctttctgc tcctgcgcga ctacttcctg cgttcttaaa
    12121 gagtcctgca agtttttcat acgcagcgcg atgttgtctt tcaggtcttt ttccagctct
    12181 acatttacct ggtcgtcaac ttgctggata tacttcgcca gctgcttctg cgcaccttca
    12241 ggtgtttcac ccacataaga caccgccagt ggtaacggct ggtttttaac ggtaggctca
    12301 atggtcagct tttctgcttc ttcctgatta tccagcgttt ccgctaatgc tgaaaatgca
    12361 gtgctgtaac gtccgataag tgaagcctga atgtcggaga ctttcggtac agccgatccg
    12421 tagatcacat tcatcgcgtt ggtataacca gcgatttgcc ccacatcagg ttgagtaacg
    12481 atagctgtgg aagtccactt ttctttggca acggccagat accctatagc cagggcaata
    12541 gccacaatga cagagatgat aattgtcatc ttgccgcgcc acaactgcac cagtaaatca
    12601 atcaaatcaa tctgttccgg gtcaaggttt tgcccagaaa cattattatt ttctactctc
    12661 atcattaccc taacggacgt tataattt
//
LOCUS       DAPHBJ010000132        12658 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.133, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000132 DAPHBJ010000000
VERSION     DAPHBJ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.133"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(59..1708)
                     /gene="actP"
                     /locus_tag="RHZ76_003218"
     CDS             complement(59..1708)
                     /gene="actP"
                     /locus_tag="RHZ76_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HDV2813810.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1705..2019)
                     /locus_tag="RHZ76_003219"
     CDS             complement(1705..2019)
                     /locus_tag="RHZ76_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HDV2813811.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDHLNNEVLHEVQ
                     AS"
     gene            complement(2220..4178)
                     /gene="acs"
                     /locus_tag="RHZ76_003220"
     CDS             complement(2220..4178)
                     /gene="acs"
                     /locus_tag="RHZ76_003220"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HDV2813812.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHINYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4571..6007
                     /gene="nrfA"
                     /locus_tag="RHZ76_003221"
     CDS             4571..6007
                     /gene="nrfA"
                     /locus_tag="RHZ76_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HDV2813813.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6052..6618
                     /gene="nrfB"
                     /locus_tag="RHZ76_003222"
     CDS             6052..6618
                     /gene="nrfB"
                     /locus_tag="RHZ76_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HDV2813814.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6615..7286
                     /gene="nrfC"
                     /locus_tag="RHZ76_003223"
     CDS             6615..7286
                     /gene="nrfC"
                     /locus_tag="RHZ76_003223"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HDV2813815.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7283..8239
                     /gene="nrfD"
                     /locus_tag="RHZ76_003224"
     CDS             7283..8239
                     /gene="nrfD"
                     /locus_tag="RHZ76_003224"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HDV2813816.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8259..9977
                     /locus_tag="RHZ76_003225"
     CDS             8259..9977
                     /locus_tag="RHZ76_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HDV2813817.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRSEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGVVVSSVSRQEISLN
                     LQPGQPVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGLDRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            9970..10353
                     /gene="nrfF"
                     /locus_tag="RHZ76_003226"
     CDS             9970..10353
                     /gene="nrfF"
                     /locus_tag="RHZ76_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HDV2813818.1"
                     /translation="MSKGLLTLLLLFTCFARAQVVDTWQFANPQQQEQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10350..10946
                     /gene="nrfG"
                     /locus_tag="RHZ76_003227"
     CDS             10350..10946
                     /gene="nrfG"
                     /locus_tag="RHZ76_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HDV2813819.1"
                     /translation="MKQPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     HGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11288..12601
                     /gene="gltP"
                     /locus_tag="RHZ76_003228"
     CDS             11288..12601
                     /gene="gltP"
                     /locus_tag="RHZ76_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HDV2813820.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
BASE COUNT         2816 a         3302 c         3543 g         2997 t
ORIGIN      
        1 tgatgcatgc tggagaggaa gagtattccc cggtcaggcg accggggaag gggagagatt
       61 aatgtgcgcg gccttgctca acgccaaagc cggtctggga gcggataaac tgcgcgcgga
      121 acagttcacg ctcgcgcgcc ccttccgctg agttatcggt tgccgagaag aaccagatgc
      181 cgaggaatgc cacggtgata gagaacagcg ccgggtattc atacgggaag atggcttttt
      241 cgtgaccaag gatctgtacc caaatcgtcg ggccgaggat catcagtact actgcggtaa
      301 tcagccccag ccagccaccc atcatcgcgc cacgcgtggt cagtttcgac cagtacatag
      361 aaagcagaat gatcgggaag ttacagctcg ccgcgatggc aaacgccagc cccaccataa
      421 aggcgatgtt ctgattctca aacagcacgc cgaggataat cgcaatcacg ccgaggatca
      481 gtacggtgat ttttgatacc cgcagctctt cacgttcggt cgcgcctttt ttgaagacgt
      541 tagcgtacaa gtcatgcgaa accgcggatg cgcccgccag cgtcagaccc gcaaccaccg
      601 cgaggatagt ggcgaaagca accgctgaaa taaaaccgag gaacaggttg ccgcccactg
      661 cattcgccag gtgaacggcc gccatgttgt taccaccaat cagatggccc gccgcgtctt
      721 tatattccgg attcgcacca accagcatga tcgcgccgaa gccgataata aaggtcagaa
      781 tatagaagta gcccataaac ccggtggcgt agaacacgct cttacgtgct tcgcgggcat
      841 cgctgactgt aaagaagcgc atcagaatgt gcggcaagcc cgccgtacca aacatcagtc
      901 ccagacccag agagagcgcg gagatcggat ctttcaccag cccgcccggc ttcatgatgt
      961 cgaccccttt cgggtgtacc gccatcgctt cgctgaacag attgttgaag ctaaagccaa
     1021 cgtgtttcat caccataaag gccataaagc tggcaccgaa cagtaacagc acggctttaa
     1081 taatttgcac ccaggtggtc gccagcatgc cgccgaacag gacatacatc atcatcagca
     1141 cgccgaccag caccaccgca atgtgatagt taaggccaaa cagcagctcg atcagtttac
     1201 ctgcgcctac catctgagcg ataaggtaaa gcgccaccac caccagagaa ccacaggccg
     1261 aaagaatacg aatcggccct tgtttcaggc ggtaagaggc tacatcggca aaggtgtagc
     1321 gtcccaggtt acgcagacgt tcggcaatca ggaacaaaat gatcggccag cccaccagga
     1381 agcccagcga gtaaattaag ccgtcatagc cggaggtaaa caccagtgcg gagatcccca
     1441 aaaatgaggc ggcggacata tagtcaccgg caatcgccag cccgttctgg aagcctgtga
     1501 tattgccgcc tgccgtgtag tagtcgctac gagaacgtac gcgttttgat gcccagtaag
     1561 taatgccgag cgtaaacacg acgaaaatca ggaacataat aatcgcctgc cagttcgttg
     1621 gctggcgctc tacggccccg ctaatagcat ccgcggcgtt agctgcgaaa gggagtgtgg
     1681 cggcaagcgc cgtcagaact cttttcatga tgcttgtacc tcatgcagga cttcgttatt
     1741 aagatggtcg aattcgccgt tcgcccgcca gatgtagata ccggtgagaa caaaggagat
     1801 cacaatcact ccaacaccaa tcggaatacc gcgtgtgacg ctggtgttcg gattcagcgg
     1861 ggtgcccagc cagccgggcg cgaaggcgat cagtaaaata aagccgatat aaactgccag
     1921 cataataatc gacaggatgg tggcaaaccg ttgccgtttt tcgactaact ccctgaaatg
     1981 cgcattgtct tctatccgct gataaatcgt accattcatc acagattctc cagaggtaat
     2041 gtagggattg ttttaattcc cgctccctta tgggagaagg ttaacgctcg ggtaaccctt
     2101 gccgaatgta ggccggataa ggcgttatac gccgcatccg gcaatcaatg cctgatgcga
     2161 cgctgtcgcg tcttatcagg cctacaaacc gttaccgact cgcatccggc aattgtgggt
     2221 tacgatggca tcgcgatagc ctgcttctct tcaagcagct tctcgactac gccaggatcg
     2281 gcaagcgtcg aggtatcgcc caggttgctg gtatcgcccg ccgcaatttt gcgcagaata
     2341 cggcgcataa ttttgccgga gcgggtttta ggcagggagt cggtccagtg cagcacgtct
     2401 ggcgtcgcaa gcgggccaat ctctttacgc acccagttgc ggacttctgc gtacagttct
     2461 ggtgacggtt cctccccgtg attaagcgtg acgtaggcgt agatcgcctg acctttaata
     2521 ttgtgcggaa tacctactac ggcggcttcg gcaatcttcg gatgcgccac cagcgccgac
     2581 tcaatctccg ccgttcccag acggtgaccg gagacgttca gcacatcgtc cacacgcccg
     2641 gttatccagt aatagccatc ttcatcgcga cgcgcgccgt cgccgctgaa atacatattt
     2701 ttgaaggtgg agaagtaggt ctgttcaaaa cgttcgtgat cgccaaacag cgtacgcgcc
     2761 tgacccggcc aggagtcggt gatcaccagg ctgccttcgg tagcgccttc cagcgggtta
     2821 ccttcgttat cgaccagcgc cggttgcacg ccgaagaacg gacgtgttgc cgaaccggct
     2881 ttcagctcgg tagcgccagg cagcggcgtg atcatgaaac cgccagtttc ggtctgccac
     2941 caggtatcga ccaccggaca tttctcgttg ccgatttttt tccagtacca ctcccacgct
     3001 tccgggttga ttggctcgcc cacggaaccg agaatacgca gcgacgatcg gtcggtgcct
     3061 tcaatcgctt tatccccttc cgccatcagc gcgcgaatcg ccgtgggcgc ggtatagaga
     3121 atattgacct gatgcttgtc caccacctgc gccatacggg caggcgtcgg ccagttcggt
     3181 acgccttcaa acatcagcgt ggtcgcaccg caggccagcg ggccgtacag caagtaactg
     3241 tgtccggtca cccagcccac atcggcggtg caccagtaga tatcacccgg atgataatca
     3301 aagacatatt taaaggtcag cgccgcgtac accagataac cgccggtagt atgcagcaca
     3361 ccttttggct taccggtaga accggaggtg tagagaataa acagcggatc ttcggcgttc
     3421 atctcttccg cctggtgctg atcgctcgct tgctcaacca ggtcgtgcca ccacaggtcg
     3481 cgcccttcct gccagtcaat tttcccgcca gtacgcttca gtaccaccac atgctctacg
     3541 ctggtgacgt tcgggttttt cagcgcgtca tcaacgtttt tcttcagcgg aatactgcgc
     3601 ccggcacgca caccttcgtc ggaagtgatc accagtcgtg agttggaatc aataatgcgc
     3661 ccggcaacgg cttccggcga gaagccgccg aaaatcaccg aatgcaccgc gccaatgcgg
     3721 gcgcaggcca gcatcgcaac cgcggcttcc ggcaccatcg gcatataaat cgccaccaca
     3781 tcaccttttt taatgcccag ctcgagcagg gtattggcga agcggcagac gtcgcggtgc
     3841 agctctttat agttgatatg tttgctctgg ctggcgtcgt cgccttccca gatgatggcg
     3901 gtacgatcgc cgttttcttg cagatggcgg tcaaggcagt ttgccgccag attcagcgtg
     3961 ccgtcctcgt accatttaat ggacacatta ccgggggcaa aggaggtgtt tttcaccttc
     4021 tggtaaggtt tgatccagtc aagaattttt ccctgttcgc cccagaaggt atcaggtacg
     4081 ttaatagatt gttgatacat cgcctcgtac tgctgagggt ttatcaggca acggtctgcg
     4141 atgttggcag gaatggtgtg tttgtgaatt tggctcatgc ttttgttctc cttgtaggat
     4201 gttaataata tgtggcataa gcgttaaatg taggggtatt ggcagttttg tttagtattt
     4261 gggcgacaga tcacgcaaaa gtagaattgt gcaaataaac ggcagggtaa tttttgaagg
     4321 tcagcaacaa aagttgatta attctttgag gaacatgcag ttatgcatgc tgttgaaaaa
     4381 gaggaagata ctgactaact ctaaagtggt attttacatg cacttacaat tgattaaaga
     4441 caacattcac agtgtggtta tttgttacac ataggggcga gcaatgtcat gacagtgtag
     4501 gtgcggttac tgtcgtgaaa aacaataaaa accgccattg caacaatggc gcaattcgga
     4561 tgaagcccct atgacaagga taaaaataaa cgcacgccgt atcttcagct tattgattcc
     4621 ttttttcttt ttcacttctg ttcacgctga acaaacggct gctcccgcaa aacctgtaac
     4681 tgtggaagcg aagaatgaaa cctttgcccc gcagcatccc gatcaatatc tctcctggaa
     4741 agccacctcg gaacagtcag agcgtgttga cgccctggcg gaagatccac ggctggtgat
     4801 cctgtgggcg gggtatccct tctcgcgcga ttacaacaag ccgcgtggac atgcttttgc
     4861 tgtgaccgat gtgcgtgaaa ccctgcgtac cggtgcgccg aaaaacgctg aagatggtcc
     4921 gctaccgatg gcatgctgga gttgtaaaag cccggatgtg gcgcgtctga tccagaaaga
     4981 cggcgaagat ggctacttcc acggtaaatg ggcgcgcggc ggtccggaaa tcgtcaacaa
     5041 cttaggttgt gccgattgcc ataacaccgc ctctccagag ttcgccaaag gcaaaccgga
     5101 gttaaccctt tcccgtccgt atgcggctcg cgcgatggaa gccattggta aaccttttga
     5161 gaaagccgga cgtttcgacc agcaatcgat ggtttgcggt cagtgccatg tggagtatta
     5221 cttcgacggc aaaaacaaag cggttaaatt cccgtgggat gacggcatga aagtcgaaaa
     5281 tatggagcag tattacgaca aaattgcctt ctctgactgg actaactccc tgtcgaaaac
     5341 gccaatgctg aaagcgcagc acccggaata tgaaacctgg acagcgggca ttcacggtaa
     5401 aaacaacgtg acctgtatcg actgccatat gccaaaagtg cagaacgccg aaggcaaact
     5461 ctacaccgac cataaaattg gtaatccgtt tgataacttc gcccagactt gtgcgaactg
     5521 ccatacccag gacaaagctg ccttgcaaaa agtggtcgcg gaacgtaagc agtcgattaa
     5581 cgacctgaaa atcaaggttg aagatcaact ggttcacgct cacttcgaag cgaaagcagc
     5641 gctggatgca ggcgcgacgg aagctgaaat gaagccaatt caggacgata tccgtcatgc
     5701 ccagtggcgc tgggatctgg cgatcgcttc ccacggcatt catatgcacg caccggaaga
     5761 aggtttacgg atgctcggta cggcgatgga taaagcggcg gatgcacgca ccaaactggc
     5821 gcgcctgctg gcgaccaaag gcatcaccca tgaaatccag atcccggata tctcaaccaa
     5881 agagaaagcc cagcaggcca ttggcctgaa catggaacaa atcaaggccg agaagcagga
     5941 cttcatcaaa acggtgatcc cgcagtggga agaacaggca cgtaaaaacg gtctgttaag
     6001 ccaataaccc cgttccgcct cgcaaggggc ggaaaacaca atggagtgaa tatgagcgta
     6061 ttacgttcgt tattaactgc cggggtgctg gcgtcgggcc tgttgtggag cctgaacgga
     6121 attaccgcta cccctgcggc gcaggcatct gatgatcgct acgaagttac ccagcagcgt
     6181 aacccggatg ccgcctgtct ggactgtcat aaaccagata ccgaaggtat gcatggaaaa
     6241 catgcttccg ttatcaaccc gaataacaaa ctgccggtca cctgcaccaa ctgtcacggc
     6301 cagccatcac cgcaacaccg cgaaggggtg aaagatgtga tgcgctttaa cgagccgatg
     6361 tacaaggttg gagagcagaa cagcgtctgt atgtcctgtc atctgccgga acagttgcaa
     6421 aaagcgttct ggccgcacga tgtccacgta accaaagtgg cgtgcgccag ctgccattcc
     6481 ctgcatccac agcaagatac gatgcagacg ttaagtgaca aaggacggat taagatttgc
     6541 gtcgattgcc acagcgatca gcgcaccaat ccgaacttta acccggcgtc tgttccgttg
     6601 cttaaggagc agccatgacc tggtctcgtc gccagtttct caccggcgtc ggcgtgctgg
     6661 cagccgtcag cggcaccgca gggcgcgtgg tggcgaaaac gttgaatatc aatggggtgc
     6721 gttacggcat ggtgcatgac gagtcgttat gcatcggctg taccgcctgt atggatgctt
     6781 gtcgggaagt gaacaaggtg ccggaaggcg tctcgcgcct gacgattatt cgtagcgagc
     6841 cgcagggcga atttcctgat gtgaaatatc gcttcttccg taagtcttgc cagcactgcg
     6901 atcatgcgcc gtgcgttgac gtctgcccga ccggtgcgtc ttttcgcgat gctgccagtg
     6961 ggattgtcga tgttaatccg gatctctgcg tcggttgtca gtactgcatc gccgcctgtc
     7021 cgtaccgcgt gcgctttatc catccggtca cgaagacggc ggacaaatgc gatttctgcc
     7081 gtaagaccaa tttgcaggcc ggtaagttgc ccgcgtgcgt tgaagcttgc ccgaccaagg
     7141 cgctgacgtt tggcaatctg gacgatccca acagtgagat ttcgcaactg ctgcgccaga
     7201 agcccactta ccgctacaag ctggcgctgg gaaccaaacc gaagctgtac cgcgtaccgt
     7261 ttaaatacgg ggaggtgagc caatgacgca gacttccgca tttcattttg aatcgctggt
     7321 gtgggactgg ccgattgcca tctacctgtt tttgattggt atttctgctg gtctggtgac
     7381 gctggccgtg ctgttacgtc gcttctaccc gcaggcgggc ggtgcagaca gtacgttgct
     7441 gcgcaccacg ctgattgtcg ggccgggcgc ggtgatcctc ggtctgttga tcctcgtctt
     7501 ccacctgaca agaccgtgga ccttctggaa gctgatgttc cactacagtt ttacctcggt
     7561 gatgtcgatg ggggtgatgc tgtttcagct ctacatggtg gtgctggtgc tgtggctggc
     7621 gaaaatcttt gaacatgatt tgcttgccct gcaacaacgc tggttgccga agctggggat
     7681 cgtgcaaaag gttctgagcc tgctgacgcc cgttcatcgc ggactggaaa cattgatgct
     7741 ggtgctggcg gtgttgttgg gggcttatac cggctttctg ctgtcggcgc tgaaatcgta
     7801 tccgttcctc aataacccga tcctgccggt gctgttcctc ttctccggca tctcgtccgg
     7861 tgcggcggtg gcgctgatcg ccatggcgat acgccaacgc agtaacccgc attccacgga
     7921 agcgcagttt gtacaccgta tggaaatccc tgtggtatgg ggtgaaatct tcctgctggt
     7981 ggcgtttttt gtcggtctgg cgctgggcga tgacggtaaa gttcgtgcgc tggtggcggc
     8041 attaggtggc ggtttctgga cgtggtggtt ctggcttggt gtcgccgggc tggggctgat
     8101 tgtgccaatg ttgctcaaac cgtgggtcaa tcgcagttcc ggcattcctg ccgtgctggc
     8161 ggcgtgtggg gccagtctgg tcggcgtgtt gatgctgcgc tttttcattc tctacgccgg
     8221 acagttgacg gtggcgtaag ccagaaaaga ggtggtttct ggacgtattc cttcctgaag
     8281 tcggttttct ggcgttgttg ttaagtctcg gggtcaacgt gttgaccccg ttgacggcct
     8341 ttgcgggagt gcggttgcgc tggcctgcca tgatgcgact cacttgcatc ggcattctgg
     8401 cgcagttcgc gctcctgctg ctcgcctttg gcgtactgac gtattgtttt ctcatcagcg
     8461 atttctcggt catttatgtc gcgcaacata gctacagcct gctgtcgtgg gaactcaagc
     8521 tggcagcggt gtggggcggc catgaaggtt cgctgctgct ttgggtgctg ctgctttccg
     8581 cctggagcgc gctgtttgcc tggcattatc ggcagcaaac cgatccgcta tttccgctga
     8641 cgctagccgt tttatctctc atgctcgccg cactgctact gtttgtggtg ctgtggtccg
     8701 atcccttcgt gcggatattt ccaccagcaa tcgaaggccg cgatctcaat ccgatgctgc
     8761 aacatcccgg tcttatcttt catccaccgc tgctttatct cgggtatggc ggtttgatgg
     8821 tggcggcgag cgtggcgctg gcgagtttac tgcgcagcga gtttgatggt gcctgcgccc
     8881 gaatttgctg gcgctgggca ctacctggct ggagcgcatt aacggcgggg atcattctcg
     8941 gttcctggtg ggcctattgc gaactgggct ggggcggctg gtggttctgg gatccggtgg
     9001 aaaacgcctc tttattaccc tggctttctg ccactgcgct gctgcacagt ttatctctga
     9061 cacgtcagcg ggggattttc cgccactggt cgctgctgct ggcgatagtt actctgatgc
     9121 tgtcgctgct gggcacctta attgtccgtt ctggcattct ggtttcggtt catgcgttcg
     9181 cgctggataa cgtccgcgcc gtgccgttgt tcagcctgtt tgcactgatt agccttgcgt
     9241 ctctggctct gtatggctgg cgagcgcggg acggtggccc ggcggtgcgt ttttcggggt
     9301 tatcgcggga aatgttaatc ctcgctacgc tgttgctgtt ttgcgcagtg ctactgatcg
     9361 tgctggtggg aacgctttat ccgatgattt acggcctgct gggctgggga cgcctctccg
     9421 ttggcgcacc ttattttaac cgcgcgacgt taccgtttgg tctgttgatg ctggtggtga
     9481 ttgtgctggc gacgtttgtc tctggcaaac gcgcgcagct tccggcgctg gtagctcatg
     9541 cgggcgtgct gttatttgcc gctggggtcg tggtttccag cgtcagccgt caggaaatca
     9601 gcctgaattt acagccgggt cagccggtga cgctggcagg atacaccttc cgttttgagc
     9661 gcctcgatct gcaagccaaa ggcaattaca ccagcgaaaa agcgatagtg gcgctgtttg
     9721 accatcagca acgcattggt gaattaacgc cggagcggcg tttttacgaa gctcgtcgtc
     9781 agcaaatgat ggaaccgtca attcgctgga acggcatcca tgactggtat gcggtcatgg
     9841 gggagaaaac tgggctggat cgttacgctt ttcgtttgta tgtacaaagc ggtgtgcgct
     9901 ggatctgggg gggaggattg ttgatgattg caggcgcatt attaagcgga tggcggggga
     9961 ggaagcgcga tgagtaaagg acttctcacg ctactgctgt tatttacctg ttttgcgcgc
    10021 gctcaggttg tagacacctg gcaattcgct aatccgcaac aacaggaaca ggcgttaaat
    10081 attgccagcc agttacgttg tccgcagtgc cagaatcaaa acttactgga atccaatgcg
    10141 ccggtggcag tcagtatgcg ccatcaggtt tacagcatgg tggcggaggg gaagaacgaa
    10201 gtcgaaatca ttggctggat gaccgaacgc tacggagatt ttgttcgcta taacccaccg
    10261 ttaacgggtc agacgctggt gttatgggcg ctgccagtgg tgttgttact actgatggca
    10321 ctgatcctct ggcgagtgag ggcgaagcga tgaaacagcc caaaataccg gtgaaaatgc
    10381 tgacaacact cacaatattg atggtatttc tctgtgtcgg cagttatctg ttaagcccaa
    10441 aatggcaggc ggtacgtgcg gagtatcagc gtcagcgcga tccgctacat cagtttgcca
    10501 gccagcaaac cccggaagcg cagcttcagg cattacagga taaaatccgc gctaatccac
    10561 aaaacagcga acagtgggcg ttactgggcg agtactatct gtggcaaaac gattacagca
    10621 attcgctgct ggcgtatcgt caggcgttgc aactacatgg tgagaacgct gaactgtatg
    10681 cggcgctggc gacggtgctt tattaccagg ccagccagca tatgaccgcc cagactcgcg
    10741 caatgatcga caaagccctc gcgctggact ctaacgaaat caccgctctg atgctgctgg
    10801 cttccgatgc gtttatgcag gcgaattacg cgcaagccat cgaactatgg caaaaagtga
    10861 tggatctcaa ctcaccccgg atcaaccgga cacagctggt cgagtcgatt aatatggcga
    10921 aactgttgca gcggagatcg gattaatcat ctggtgatga agattatgtc attgagctgc
    10981 ataaaaaata atcgaatgaa catatgccaa aaataatcac taatcagtat tattgcagat
    11041 taaacaaata aaaatctttc cataacaaat ggttattcat taatcctgcc ataaactcgc
    11101 gttattatgc attaatgcag cgaaaagctc tgttgttaaa gggttgcgca acataccgcg
    11161 caaatgatac tgatcataag cgttaaaaaa atctacaaac caacgcaaca caattcatgc
    11221 cctggcagta tgtcacgttc tcgcgtttct gaacggggaa cggcgctcca ttgaggaagt
    11281 tattctgatg aaaagtataa aattcagcct ggcctggcag attctgtttg ctatggtgct
    11341 gggcattctc ctgggaagct acctgcacta ccatagcgac agccgcgact ggctggtcgt
    11401 caatttgctc tctccggcgg gtgatatctt catccatctg atcaaaatga ttgttgtgcc
    11461 gattgtgatc tccacgctgg tggtgggtat cgcgggtgtt ggtgatgcca aacagctcgg
    11521 gcgtattggc gcgaaaacca ttatttactt cgaggtgatc accaccgtcg ccatcatttt
    11581 ggggatcact ctggcgaacg tcttccagcc cggtgccggg gtggatatgt cgcagctggc
    11641 gaccgtcgat atctcgaaat atcagagcac tacggaagcg gtacaaagca gttcccacgg
    11701 cattatgggc acgattttgt cgctggtgcc gacgaacatt gtggcgtcga tggcgaaagg
    11761 cgaaatgctg ccgatcatct tcttctcggt gctgtttggt ctggggcttt cctccctgcc
    11821 cgcgacgcat cgtgaaccgc tggtgaccgt gttccgctcc atctctgaaa ccatgtttaa
    11881 agtgactcac atggtgatgc gttatgcacc ggtgggtgtg tttgcgctga ttgcggtgac
    11941 ggtggctaac tttggtttct cttctctgtg gccactggcg aaactggtgc tgctggtgca
    12001 tttcgccatt ctgttcttcg cgctggtagt gctgggaatt gtggcgcgcc tgtgcgggtt
    12061 aagcgtctgg atcctgattc gtattctgaa agatgagctg attctggcgt actccactgc
    12121 cagctctgaa agcgtactgc cgcgaattat tgagaagatg gaagcctacg gagcaccggc
    12181 gtcgatcacc agcttcgtgg tgccgaccgg ttactctttt aaccttgatg gttcgacgct
    12241 gtatcaaagt attgccgcta ttttcatcgc tcagctgtat ggcattgacc tgtccatctg
    12301 gcaggaaatc attctggtgc tgacgctgat ggtgacctcg aaagggattg ctggcgtgcc
    12361 gggagtgtcg tttgtggtgt tgctggcaac gctgggtagc gtaggtattc cgctggaagg
    12421 tctggcgttt attgccggtg ttgaccgtat cctcgacatg gcgcgtactg cgctgaacgt
    12481 tgtgggtaat gcgctggcgg tgctggtgat tgccaagtgg gaacacaaat ttgaccgcaa
    12541 aaaggcgctg gcttatgagc gtgaagtgct gggtaaattt gataaaactg cggatcaata
    12601 attgaagatt gccggggata tccacccggc aatgtgtgga tgcctgatgc gacgctgg
//
LOCUS       DAPHBJ010000133        12059 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.134, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000133 DAPHBJ010000000
VERSION     DAPHBJ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.134"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            3..707
                     /locus_tag="RHZ76_003229"
     CDS             3..707
                     /locus_tag="RHZ76_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV2813821.1"
                     /translation="MNLLSRLTPLLFVFAPFFAQSNMTVYPMAVSINSQGEGNVRVIS
                     KSNEVQYIKATVFRIDNPSTPQEQEVEIKSGDATHLVVMPPKFALPAGSSKTVRFVAM
                     EPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKLEVNA
                     AQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKIVVKY
                     NNWITKDNGEFELAVH"
     gene            737..1237
                     /locus_tag="RHZ76_003230"
     CDS             737..1237
                     /locus_tag="RHZ76_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2813822.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1328..4012
                     /locus_tag="RHZ76_003231"
     CDS             1328..4012
                     /locus_tag="RHZ76_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HDV2813823.1"
                     /translation="MDKKLLALLILASLSPAEAALTKIPAGFEVIAQGQQEYIEVYFS
                     GKNLGKYYAMVNFDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQASDDESSAYQLGVRLPLHPQYELYAGLANA
                     DNVSAFELGNDWTANLGGAGNLAISASVFRNDDGGKGDMQQANWSNSGWPTLGFYRTN
                     SDGDACATDSRESYNALSCYESISATVSQNFVGWNMMLGYSRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSDQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPASVEK
                     FPLVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4009..5097
                     /locus_tag="RHZ76_003232"
     CDS             4009..5097
                     /locus_tag="RHZ76_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HDV2813824.1"
                     /translation="MRNRLIAAILGLCGAVTGVQAAPNVTSEITYDLASGRADYYFWN
                     EEPPPEVSYSTTFSFFQCSYPDSQQTCTSAGNTSVVQIYLTEKRSGMRWPVKLKGYMT
                     VQVWEDGPCKGWYDKKRLDDGTGYQCKDTINNVGYLAKTKVLTLYIEQEEMKKLPIGG
                     LWEGKVKLHFSYPATDYQADIKLNVLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVE
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(5185..6483)
                     /gene="exuT"
                     /locus_tag="RHZ76_003233"
     CDS             complement(5185..6483)
                     /gene="exuT"
                     /locus_tag="RHZ76_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HDV2813825.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            6966..8378
                     /gene="uxaC"
                     /locus_tag="RHZ76_003234"
     CDS             6966..8378
                     /gene="uxaC"
                     /locus_tag="RHZ76_003234"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDV2813826.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8393..9880
                     /gene="uxaA"
                     /locus_tag="RHZ76_003235"
     CDS             8393..9880
                     /gene="uxaA"
                     /locus_tag="RHZ76_003235"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDV2813827.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVRLRQDVARGHKF
                     ALTNIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9963..10514
                     /locus_tag="RHZ76_003236"
     CDS             9963..10514
                     /locus_tag="RHZ76_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HDV2813828.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPSAVDERG"
     gene            complement(10519..11763)
                     /gene="sstT"
                     /locus_tag="RHZ76_003237"
     CDS             complement(10519..11763)
                     /gene="sstT"
                     /locus_tag="RHZ76_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDV2813829.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LFVLVGCMLMVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         3230 a         2854 c         3082 g         2893 t
ORIGIN      
        1 aaattaatct cttatcacgg cttacgccgc ttttattcgt gttcgctcct ttttttgcac
       61 agtcaaatat gactgtttat cctatggctg tttccattaa tagtcagggg gaaggtaacg
      121 ttcgcgtaat atctaaaagt aatgaagttc aatatataaa agcgacggta tttcgtattg
      181 ataatccttc aacgcctcaa gagcaggaag ttgaaataaa atccggagat gccactcatc
      241 ttgtagtgat gccgcctaag tttgcgctgc cagcgggtag cagtaagact gtgcgctttg
      301 ttgcaatgga accagagcaa aaagaaaaaa attatcgcgt taaatttgaa gcggttccca
      361 gtattgatga cgttgccacg gataaaaaag atctctctat gcagttaaca gttaacttaa
      421 tttgggggat tgttgttagt gtcccacctc agcaacctat tgctaagtta gaagtaaatg
      481 ctgcccaaaa attagttaat gcagggaatc aacgcttaaa gattttaacg atcgcttatt
      541 gtaaaaataa tagcaaagaa aattgtaaga tacagactgt aaataaaaat atcttccctg
      601 gtcaggaaag aaatcttgaa agcatatctg gctacgacaa aatcgttgtc aaatataata
      661 actggatcac caaagataat ggcgagtttg aactggcggt ccattaatta tcattgtaat
      721 aaacgaagga atagtaatga aaaaggtatt tgcaaaatct cttctggtcg cagcgatgtt
      781 ttctgttgca ggctccgcat tagcagtaca aaaggatatc actgtaacgg ctaacgttga
      841 tgccgctctg gatatgacac agaccgataa cactgcgctg ccgaaagcag tagaaatgca
      901 atatctgccg ggtcagggtc tgcaatctta ccagttaatg accaaaatct ggtctaacga
      961 cgttaccaaa gatgtaaaaa tgcagttagt ttccccggcg caactggttc aaagcctgga
     1021 cgctagcaag attgttccgc tgacagtaac ctggggcggc gaagaaatta aagctgatgc
     1081 ggcaaccact tttaccgcaa ccaaaatctt tgcttccgat gcgttaacta atggttctct
     1141 ggctaaaaat ctgatgtttg ctcaaaccac caaaggtgtt ctggaaacag gtatctaccg
     1201 tggcgtagta agcatttatc tgtctcagga tatctaatcc ggataacaga attaacacat
     1261 aacaaagggg ggctagccct cctttatttt aactgtttta gtatttgtta tggatttcag
     1321 gattgccatg gataaaaaat tactggctct tttgatcctg gcgagtctca gcccggcaga
     1381 ggcggcatta accaaaatcc ccgcagggtt tgaggttatt gctcagggac agcaggagta
     1441 tatcgaggtt tatttttcag ggaaaaatct cggtaaatat tatgcaatgg ttaattttga
     1501 taccgtaaca tttcttgatc cagcaagttt atataacaag ctggaactgg atgtcgacga
     1561 tcagaaaatc gcgcatatag tgaaagaaaa attatcgcag ccgctagctc gccacggtga
     1621 attggcttgc ggttatgtac gtactgactc agggtgtggt tttctgaata ccgatacgct
     1681 ggaaataatc tataatgatg aagaaagttc ggcaacgttg tttattaatc cgcaatggaa
     1741 ttcagctttc gatgcgaagt cattatattt aaatccagac aaaaatacgg ttaacgcttt
     1801 tatacatcag caagacatca atgttctggc acaggatgat taccaatcgt tgtctattca
     1861 gggaaacggt gcgctgggaa taacagaaaa tagctatatt ggtgcacact ggaatttcaa
     1921 cggttatgat gcagatgatg tcagtgacag taatgctgat gtcagcgatc tctattatcg
     1981 ttatgatttt ttacgtcgtt attatgtgca ggcggggcgc atggacaacc gcacgctatt
     2041 taatgcacaa ggcgggaact ttacctttaa ctttttgcca ctcggtgcaa tcgacgggat
     2101 gcgtatcggg tcgactctta gctatttaaa ccaagcgcaa agccagcagg gaaccccggt
     2161 aatggtcctg ctttcgcgca attctcgtgt tgacgcttat cgtaatgagc aacttctggg
     2221 atcgttttat ctcaatagtg gttcgcaatt tattgatacc agttcctttc cgccgggtag
     2281 ctatagcgta gcgttaaaag tctatgaaaa taaccaactc acccgcaccg agctagtacc
     2341 gtttaccaaa acaggcggtc tgacggacgg aaatgcgcaa tggttcttac aggcaggtaa
     2401 aactacatca caggcttctg atgatgaaag ttcagcttat caactggggg tacgcctgcc
     2461 attacatccg caatatgagc tctacgcagg gctggcgaat gccgataatg tgagtgcttt
     2521 cgagttaggt aatgactgga cggcaaattt aggcggggca gggaatcttg caatcagcgc
     2581 cagcgtgttc cgtaacgatg acggcggcaa aggtgatatg caacaggcca actggagtaa
     2641 ttcgggatgg ccgacgttgg gcttttatcg gaccaactct gacggcgatg cttgtgcaac
     2701 cgacagcaga gagagctata acgccttaag ctgttatgaa agtatttccg cgacggtttc
     2761 acagaatttt gtcggctgga atatgatgct gggttattcc cgcacacaaa ataacactga
     2821 tgatagtttg cgttgggata aacagcagag ctttgaaaat aactatcttc gccagacaac
     2881 tgcgcaaagt atctccgaaa ctgtacaact tagcgcttcc cgcgcttttg tgatgcgtga
     2941 ctggatcttg agtacttcgg ttggtgtttt ccatcgtaat gacaacggtg gcgataacga
     3001 cgacaacggc ttgtacttat cgttttcgtt atctgacacg ccaacgatgg acagcaataa
     3061 caacagccat tcaaccaatg tttctacgga ttatcgttat agcgatcagg atggcgatca
     3121 aacgtcatgg cagttatccc atacttttta taacgattca ttcagccata aagaacttgg
     3181 cgtaaccgtt gggggcctga acaccgatac cataaacagc gcggttaacg ggcgttggga
     3241 tggtcaatac ggaaatgtct acgctaccgt atctgacagt tatgaccgta agaatcatga
     3301 tcatctctcg gcctttacgg ggacttacag ctctacactg gctgtcagtc gctatggcgt
     3361 taatttgggt gccagtggta cagacgattt gctgggtgcg gtattggtgg atgtgaaagg
     3421 cttctctgaa caggatgaag agagtcagga tctgcaactc gaagcgcggg tggcaggcag
     3481 ccgaacgttg cagcttggtc aaagcgacag tgtgttgttc ccttatcctg gatttcagtc
     3541 tggttttgtt gaggttaacg acagtagcca gggcaatcaa caagggacaa caaacatcat
     3601 taacggtgcg gggaatcgtg aattaatgtt gttgcctggc aaactgcgct atcgcgaagt
     3661 gtctgccagc tttaattaca actatatcgg tcgcttgtta ttaccagcat cggtagagaa
     3721 attcccgctg gttggtctga atagcgccat gttactggta gctgaagatg gcggatttac
     3781 acttgaaatt aacggtagcg aaaaagagct atatctgctt tccgggcagc aattccttaa
     3841 gtgtccgctg agtgttgtaa agaaacgcgc cagcattcgt tacagcggag atgtcacatg
     3901 tagtgtggtg acttattcac aattaccgga gtccattcag gttcaggcac agttgaaaca
     3961 gcctaaatta cgtggaaacg ttcagacggc gcaaagggag gttgcaccat gagaaatcga
     4021 ttgattgcgg cgatattggg cttgtgtggt gcggttactg gcgttcaggc agctcctaac
     4081 gtgaccagtg aaattacgta cgatttggca tctggcagag cggattatta cttctggaat
     4141 gaggagcccc caccggaggt gagttacagt acaacatttt cattttttca atgtagctac
     4201 cctgattcac agcagacttg tacatcagca ggtaatactt ctgtcgtgca aatttatcta
     4261 actgaaaaac gcagcggtat gcgctggccg gttaaactga aagggtatat gacagttcag
     4321 gtgtgggagg acggaccgtg taaggggtgg tacgataaga aaaggctgga tgatgggacg
     4381 ggttatcaat gtaaagatac gattaataac gttggttatc tggctaaaac aaaagtttta
     4441 actctgtata ttgagcaaga agaaatgaag aaactgccga ttggcggttt atgggaaggg
     4501 aaagttaaac tccattttag ctacccggca acagattatc aggctgatat taagcttaat
     4561 gttctcgacc ccaaccatat cgacgtgttc ttcccggagt tcgcccacgc cacgccacgg
     4621 gtgcagttag acttgcatcc aacagggagc gttaatggca gcaactacgc gcaagatctg
     4681 accatgttgg acatgtgctt gtacgatggt tttaacggta atgccatcag ttatgaaatc
     4741 atgctcaaag atgaagggcg acccgccgca gggcgcagag acggtgactt ctctatctat
     4801 cgtcagggag gaaccaccac cgacgaggga gaacgcattg attaccgggt caaaatgtac
     4861 aacccggaaa ccggtgggca aattgatgtg cgcaataatg aaaatatggt ctggaacagc
     4921 attaacctga aacgtgtgcg tccggtcgta ttgccaggga tccgctatgc cgtaatgtgt
     4981 gtgccaacgc cattaacgct ggcagtagaa aaatttagcg tgatggacaa acaggctgga
     5041 tattacatgg ggaaattgtc ggtaatcttt acgccttcct tgccaaccat caattaaccc
     5101 tgcaaaccgg acaaacgctt ttgtctatat aaacaacgaa gccgcctgat aaggcggctt
     5161 cgtcattaca acttacgtta aatgttaatg ttgcggtgcg ggatcgttat gtgtttcctg
     5221 cgccacctca atcgccggtt tgttctgcaa cacggtccag ataaccagcg cacctaacag
     5281 gtcgaacact gccagtactg cgaacagcgg gctgaagccg atggtgtcag ccagtgcacc
     5341 gacaaccagc gcaaatagcg tacttgccag ccatgcggac atcccggtta aaccgttcgc
     5401 cgttgccact tcgttacgac caaacacatc ggaagagagc gtaatcagcg cgccagacag
     5461 tgcctggtgg gcaaaaccac cgatacacag cagcataatt gcgacatacg ggttggtgaa
     5521 caggccgatc atacccgggc caatcatcag cactgcaccc agcgttacga ccatcttacg
     5581 ggaaacgatc aggttcacac caaaccaacg ctggaacagc ggcggcaggt aaccaccgag
     5641 gatacaaccg aggtcagcaa acagcatcgg catccaggcg aacatcgcga tctctttcag
     5701 gttaaagccg taaactttaa acatgaacag cgggatccac gcgttaaaag taccccaggc
     5761 cggttctgcc aggaaacgcg gcagcgcgat accccagaac tgacggttac gcaggatctg
     5821 accaacggac attttcttcg ccgtgctcac ctggtgctgg gcttcctgac cattaataat
     5881 atagtcgcgt tcttcatcgg tcagatgctt ctggtcgcgc ggatgtttat agaaaatcag
     5941 ccatgccatc gcccagataa agctcaatgc accggagatg ataaatgcca tctgccagct
     6001 gtgcattacg attgcccata ccaccagcgg cggcgcaatc atcgcaccaa tcgaagaacc
     6061 tacgttaaag tagcctactg cgatggaacg ctctttcgcc gggaaccatt cggagctggc
     6121 tttcagaccc gccgggatca tcgctgcttc cgcggcaccg accgcaccac gagcaacagc
     6181 caggccaccc cagctacctg ccagcgcggt tgcaccacag aacacggccc acagcacagc
     6241 aaacattgca taaccgattt tcgtacccag cacatccagt acataacctg ctaccggttg
     6301 catgaccgta taagcagcag aataggctgc gatgatatag gaatactgtt gggtggagat
     6361 gtttaactct tccatcagag ttggcgcagc tgccgccaca gtgttacgcg tcaggtaacc
     6421 aagcacggtg ccgagcgtca ccagtgcgat catataccaa cgtaaccctt taattttacg
     6481 catgtaaaac ctcatcgttt tgttatatcc gcaggaagcg cggccatcat cccgccagag
     6541 gctgaagatg ttagttacga ccgggtaacc gaaaaaaaac cggcacgccc cgaacgttgc
     6601 catgaaagtt tcgtgctaat tcggcttccg taccggttaa cccgattcat ctaacaaatg
     6661 aatgaatcgg caatttgcat tccgtcgact tatgatttgc gacggcagaa agataacttg
     6721 tcatacaact ttaaaaggtg agagccatca caaatgtgcg aatatttgta gggacattac
     6781 ctgacgacag cgaggccagt actggcgcgg cctgcagcga gatttaccac tttgagagta
     6841 atttttttaa ctacgtttat tgatctaact cacgaaaata tcttcggact ctggaaattg
     6901 gtgtgataac tttgtcagca tcgcaccata agcaagctag ctcactcgtt gagaggaaga
     6961 cgaaaatgac tccgtttatg actgaagatt tcctgttaga taccgaattt gcccgccgtc
     7021 tgtatcacga ctacgcaaaa gaccagccga ttttcgatta ccattgccat ttgccgccgc
     7081 agcagattgc ggaagactat cgttttaaaa acctgtatga catctggctg aaaggcgatc
     7141 actacaaatg gcgcgctatg cgtaccaacg gtgtggccga gcgtctgtgt accggtgatg
     7201 cgtctgaccg tgaaaaattt gacgcctggg cggcgactgt tccgcacact atcggcaacc
     7261 cgttatacca ctggacgcac ctcgaactgc gccgtccgtt tggtatcact ggcaaattgc
     7321 tttctccgtc aactgccgat gaaatctgga acgaatgtaa cgaactgctg gcgcaggata
     7381 acttctccgc gcgcggcatc atgcagcaga tgaacgtgaa aatggtcggc accaccgatg
     7441 acccgatcga ttctctggag catcacgcag agatcgccaa agacggctct ttcaccatca
     7501 aagtgctgcc gagctggcgt ccggacaaag ccttcaacat cgaacaggcg acctttaacg
     7561 actacatggc gaagctgggc gaagtttccg ataccgacat tcgccgcttt gctgacctgc
     7621 aaactgccct gaccaaacgt ctggatcact tcgccgctca cggctgtaaa gtgtctgacc
     7681 acgcgctgga tgtggtgatg tttgctgaag cgaacgaagc ggaactggac agcattctgg
     7741 cgcgccgtct ggctggcgaa accctgagcg agcacgaagt ggcgcagttc aaaactgcgg
     7801 tactggtgtt ccttggtgcc gaatatgcac gtcgcggctg ggtacagcag taccacattg
     7861 gcgcgctgcg taataacaac ctgcgtcagt tcaaactgct ggggccggat gtaggctttg
     7921 actccatcaa cgaccgtccg atggcggaag agctgtctaa gttgctgagc aagcagaatg
     7981 aagaaaacct gctgccgaaa accattctct actgcctgaa cccgcgcgat aacgaagtgc
     8041 tgggcaccat gatcggtaac ttccagggcg aaggtatgcc gggcaaaatg cagttcggtt
     8101 ccggctggtg gtttaacgat cagaaagacg gtatggaacg tcagatgacc caactggcgc
     8161 agctcggttt gctgagccgc tttgtcggta tgctaactga cagccgtagc ttcctgtcat
     8221 acacccgtca cgaatacttc cgccgcattc tgtgccagat gatcggtcgc tgggtggaag
     8281 cgggcgaagc accggcggac atcaacctgc tgggcgagat ggtgaaaaat atttgcttta
     8341 acaatgcgcg tgactacttc gccattgaac tgaactaagg tctggggttg atatgcaata
     8401 catcaagatc catgcgctgg ataacgtcgc ggtcgcttta gcggatttgg ctgaaggcac
     8461 agaagtcagt gtcgataacc aaactgttag gctgcgccag gatgttgctc gtggacataa
     8521 atttgcgtta acgaatatcg caaaaggggc caacgtcatc aaatatggcc tgccgattgg
     8581 ttatgcattg gcggatattg cggcggggga acacgttcac gcccacaata cgcgcacgaa
     8641 tctgagcgat ctggatcagt atcgctatca acctgatttt caggatctgc ctgcgcaagc
     8701 ggcagatcgt gaagtgcaga tctatcgtcg cgctaacggc gatgtcgggg tgcgtaatga
     8761 gctgtggatc ctgccaaccg tgggctgtgt caacggcatc gcgcggcaga tccaaaatcg
     8821 tttcctgaaa gaaaccaaca acgccgaagg taccgacggc gtgttcctct tcagccacac
     8881 ctacggctgc tcacagctgg gcgacgatca cattaatacc cgcaccatgc tgcaaaacat
     8941 ggtgcgccac ccgaacgcag gcgcagtgct ggtgattggt ctgggctgtg aaaacaacca
     9001 ggttgccgca ttccgtgaaa cgctgggcga tatcgatcct gaacgcgttc atttcatgat
     9061 ctgccaacag caggatgatg agatcgaagc cggaatcgag catttgcatc agctgtataa
     9121 cgtgatgcgc aacgataaac gcgagccagg caaactcagc gaactgaagt ttggtctgga
     9181 gtgcggtggt tctgacggtc tttctggtat tactgctaac ccgatgctgg ggcgtttctc
     9241 tgactacgtg attgctaacg gcggtactac cgtactgacc gaagtgccgg agatgtttgg
     9301 tgcagagcag ttgctgatgg accattgccg cgacgaagca acgtttgaaa aactggtcac
     9361 catggtcaac gacttcaaac agtactttat tgcccatgat cagccgatct atgaaaaccc
     9421 atcgccgggg aacaaagcgg gcggtatcac cacgctggaa gacaaatcac ttggctgtac
     9481 ccagaaagcg ggttccagcg tcgtggttga cgtgctgcgt tacggcgagc gtctgaaaac
     9541 gccagggctg aacttgttaa gtgcgccggg taacgatgcc gtagcgacca gcgccctggc
     9601 gggtgcgggc tgccatatgg tgctgttcag tactggtcgt ggcacgccgt atggtggatt
     9661 tgtgccgacg gtgaaaatcg ccaccaacag tgaactggcg gcgaagaaaa aacactggat
     9721 cgactttgac gcgggtcagt tgatccacgg taaagcgatg ccgcagttgc tggaagaatt
     9781 tatcgacacc atcgttgagt ttgccaacgg taagcaaacc tgtaacgagc gtaacgactt
     9841 ccgcgaactg gcgatcttca aaagcggcgt aacgctataa agtcgaaatg agttgttaat
     9901 aaaaaacggc gtttcataat gtgaagcgcc gtttgctttt ccatacctgc taaggattta
     9961 tcatgaccgc gtattggctg gcccagggcg tgggtgtcat cgcctttctg attggtatca
    10021 caacattttt caatcgtgac gaacgtcgct tcaaaaagca gctttcggtc tatagcgccg
    10081 ttattggcgt acattttttt cttctgggca cctatcccgc tggtgccagt gccatcctta
    10141 atgccattcg tacattgatt accttacgca cgcgcagctt atgggtaatg gcgattttta
    10201 ttgtgctgac tggcggaatt ggcctcgcga agttccatca tcctgtcgaa ctattgccgg
    10261 ttatcggcac gattgtcagt acctgggcgc tattccgctg taaagggctg accatgcgct
    10321 gcgtaatgtg gttttcaacg tgttgctggg tgattcacaa cttctgggcg gggtcgatag
    10381 gcggcacgat gattgagggt agttttctgc ttatgaatgg cctgaatatc attcgtttct
    10441 ggcggatgca gaaaagggga attgatccgt ttaaagttga gaaaacccct tccgccgtag
    10501 acgaaagggg ttaaacaatt aattacgcag ggcgctattt gccagacgat cgtcttctgc
    10561 ctggcaagct gccgcagtga acagcacgtc agttgaagag ttcagcgcgg tttcgcaaga
    10621 gtcctgcaat acgccgatga taaagccgac ggcaaccacc tgcatggcga tatcgttcga
    10681 aataccgaac atattacagg ccagtgggat cagcagcaga gacccccccg ccacgccgga
    10741 tgcgccacag gcacacagag aagccaccac gctcaacagc agcgccgtgg gcagatcgac
    10801 cggaataccc agcgtattaa ccgcagccag cgtcaacacg gtaatagtga ttgctgcgcc
    10861 cgccatattg atggtggctc ccagcggaat agaaacggaa taggtatcgc gatccagatt
    10921 cagcttttca cacagcgcca tattcaccgg aatgttcgct gcagagctgc gggtgaagaa
    10981 ggcatacaca ccgctttcgc gcaggcacag cagcaccagc gggaacgggt tacgacgaat
    11041 tttccaccac accagcaatg ggttaaccac cagcgccacc attaacatgc agccaaccag
    11101 cacgaacagc agttgcgcgt agccccacag tgtggagaaa ccggtggttg ccagggtaga
    11161 agaaaccaga ccaaaaatac caatcggtgc gaagcgaatg accagtttca ccataaaggt
    11221 gacggcattc gacatatcgt tgaccaggtt tttggtggtc tcgttaccgt gacgcagtgc
    11281 gaagccgagg ccgatcgccc acaccagaat cccgatgtag ttacctttca gcagcgcgtc
    11341 gatggggttg gaaaccatgc tcattaccag cccgcgcatc acttcgacaa tgcctgacgg
    11401 cggcgaaata tcacccgcgc tactggataa atgcagggta gaagggaagg caaagctgaa
    11461 gactactgcg gccagagcag cagagaaggt gcccagtaga tagaggaaca aaatagggcg
    11521 gatattggtt ttctgaccgt gctggtggtt agcaatagat gccattacca gcatcaacac
    11581 caggatgggg gcaacagctt tcagtgcgcc gacgaacaaa gtacctaaca gaccaacagc
    11641 ttccgccgcg ggttttgaga tccatgccag aagaatcccc agaacaaggc cgaccaggat
    11701 ttgttttacc aggctgccat gagccagacg ccggaatagc cccggtgaac gttgcgtagt
    11761 catttttcga tcctttcatt gtgttgtcgc acatccctgg tgcgttctgt cgacgcatct
    11821 ttcaggatgt aacaaagtgt ttgcaaggtc gagtataagg aaagttacag cttcgggaag
    11881 cgaaaaatgc tggattttaa agagaggtca tgttttgcaa cttaattgtt acatttgttt
    11941 gacataaaca gagggaagga ctggctacta aggctgatta gataacatat gggcaaaact
    12001 atttggaata tgagaacggt tgcgctcctt taatgtgcta tgccatataa agtcactgg
//
LOCUS       DAPHBJ010000134        12014 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.135, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000134 DAPHBJ010000000
VERSION     DAPHBJ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.135"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            5..607
                     /locus_tag="RHZ76_003238"
     CDS             5..607
                     /locus_tag="RHZ76_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HDV2813830.1"
                     /translation="MNINYPAEYEIGDIAFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(609..1130)
                     /gene="ruvC"
                     /locus_tag="RHZ76_003239"
     CDS             complement(609..1130)
                     /gene="ruvC"
                     /locus_tag="RHZ76_003239"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDV2813831.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1165..1905)
                     /locus_tag="RHZ76_003240"
     CDS             complement(1165..1905)
                     /locus_tag="RHZ76_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV2813832.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1934..2386)
                     /gene="nudB"
                     /locus_tag="RHZ76_003241"
     CDS             complement(1934..2386)
                     /gene="nudB"
                     /locus_tag="RHZ76_003241"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HDV2813833.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2504..4276)
                     /gene="aspS"
                     /locus_tag="RHZ76_003242"
     CDS             complement(2504..4276)
                     /gene="aspS"
                     /locus_tag="RHZ76_003242"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDV2813834.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEDILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4586..5152
                     /locus_tag="RHZ76_003243"
     CDS             4586..5152
                     /locus_tag="RHZ76_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDV2813835.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            5149..5967
                     /locus_tag="RHZ76_003244"
     CDS             5149..5967
                     /locus_tag="RHZ76_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDV2813836.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            6020..6415
                     /locus_tag="RHZ76_003245"
     CDS             6020..6415
                     /locus_tag="RHZ76_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HDV2813837.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6456..7199
                     /gene="cmoA"
                     /locus_tag="RHZ76_003246"
     CDS             6456..7199
                     /gene="cmoA"
                     /locus_tag="RHZ76_003246"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HDV2813838.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7196..8167
                     /gene="cmoB"
                     /locus_tag="RHZ76_003247"
     CDS             7196..8167
                     /gene="cmoB"
                     /locus_tag="RHZ76_003247"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HDV2813839.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8332..10761)
                     /gene="torZ"
                     /locus_tag="RHZ76_003248"
     CDS             complement(8332..10761)
                     /gene="torZ"
                     /locus_tag="RHZ76_003248"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HDV2813840.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAIQA"
     gene            complement(10786..11886)
                     /locus_tag="RHZ76_003249"
     CDS             complement(10786..11886)
                     /locus_tag="RHZ76_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDV2813841.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQTATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2762 a         3201 c         2914 g         3137 t
ORIGIN      
        1 caacgtgaat attaattacc ctgctgaata tgaaattgga gatatcgcct ttacatgtat
       61 aggtgctgcc ttatttggtc aaatatcagc tgcatcaaat tgttggagta atcacgtcgg
      121 gatcattatc ggtcataacg gtgaagactt cctggttgca gaaagccggg ttcccctctc
      181 aaccatcact acgctatccc gttttattaa acgctctgct aatcaacgct atgctataaa
      241 gcgattagac gctgggctga cagaacaaca aaaacaacga attgttgaac aggttccctc
      301 ccggctacgc aaactttacc acaccggttt taaatacgaa tcttcgcgcc agttctgttc
      361 aaaatttgtc tttgatattt ataaagaggc gctatgtatt ccggtgggtg aaatagagac
      421 gtttggagaa ttgttaaata gcaatccgaa tgcaaaactc actttctgga aattctggtt
      481 cctgggttct attccgtggg agcgtaaaac cgttacgcca gccagtttgt ggcatcatcc
      541 gggtttggtg ttgattcatg cggagggagt ggaaacgcct cagccggaac tgaccgaggc
      601 ggtataactt aacgcagtcg ccctctcgcc aggttcagcc gcgattcgct catctgcatc
      661 gcattctgac taacgtggca gtgggtgatg gcaatcgcca acgcatcagc ggcatccgcc
      721 tgtggattag cgggcagttt cagcaaggtg cggaccatat gctgcacctg gcttttttcg
      781 gcgctaccaa tacccaccac cgtttgcttc acctgacgtg cagcatattc aaataccggc
      841 aactcctgat tcaccgccgc cacaatcgcc acgccgcgcg cctggcccag tttcagggct
      901 gagtcagcgt tctttgccat aaagacttgt tcaatggcga aataatcagg ctggaactgg
      961 gtgatgattt ccgtcacgcc cgcatagatg agtttcagac gagacggtaa atcatccact
     1021 ttggtgcgta tgcatccgct acccaggtag gacagttgcc tacctacctg gcggatgacg
     1081 ccgtagccgg tcacgcgcga acccggatca atgccgagaa taatagccat cacgcgtctc
     1141 cgttttgctg tttagcaggc ctcatcagag agtcgctgcg acctcatcag agatttcacc
     1201 gttatggtaa acttcctgca cgtcgtcgca atcttccagc atatcgatca gacgcatcag
     1261 tttcggtgcg gtttctgcat ccatatcagc tttggtagac gggatcatgg aaacttccgc
     1321 gctatctgct ttcagacctg ccgcttccag agcgtcgcgc actttaccca tttcttccca
     1381 tgcagtgtag acatcaatcg cgccgtcatc ataggtcaca acgtcttcag cacctgcttc
     1441 cagtgctgct tccatgatgg tgtcttcatc gcctttctcg aaggagatca cgcctttttt
     1501 gctgaacaga taagctacgg aaccatcagt accgaggtta ccgccacatt tgctgaatgc
     1561 atgacgcact tcagcaacgg tacggttgcg gttgtcagac agacattcaa tcatgattgc
     1621 cgtgccgcca ggaccgtaac cttcgtagat gatggtttcc atgtttgcat catcatcacc
     1681 gcccacacca cgtgcaattg cgcggttcag tgtgtcgcgg gtcatgttgt tagacagtgc
     1741 tttatcaatt gctgcacgca gacgcgggtt agcgtccgga tcgccaccgc ccagcttagc
     1801 cgcggttacc agctcacgaa tgattttagt gaagatttta ccgcgcttag catcctgcgc
     1861 agctttacga tgtctggtgt tggcccattt actatgacct gccataaaaa tatctccaga
     1921 tagccctgcc tgttcaggca gcgttaatta caaactgttc aatcgcctgc cggttgctcc
     1981 aggacttagt gagcgccgcc gcagcaggcg catcaagcca cttgtaagcc agatgttcag
     2041 tgaaaacgat ctgccgctcg tgcggaagcg caagacagaa ccatgattcc gtattacgcg
     2101 tcacgcccgg cgcatagcga tgacgtaaat gtgaaaaaat ttcaaactct accgtgcgct
     2161 gacagtcaat taaggtcagt tgttcagcga caacatcaat ggtgacctct tcctttactt
     2221 cgcgcatggc agcttgcggc gcggtttcac cctcttccac gctgccggtt accgactgcc
     2281 agaaatcggg atcgtcacgc cgctgcaaca tcagcacccg tttcgtatct tgtgcgtaga
     2341 tgaccactaa gatcgaaacg ggacgcttat acactttatc cttcacgctg cctcttcgtt
     2401 gactgccttc gctcatccca ttcacatagt tatctatgct cataggagtt cactcagttg
     2461 ccgcctcgat gcaacgcgaa tgatttcgtg tatttgagtc atatcagtta ttctcagcct
     2521 tcttcacaac ctgaatgctc agctcagcca gtgcagtcgg gttagcaaag ctcggtgctt
     2581 cagtcatcag acacgctgcc gccgtggttt tcgggaaggc gataacgtca cggatattgt
     2641 cggtgccggt cagcagcatg gtcagacggt caagaccgaa tgccagacct gcgtgcggcg
     2701 gagtaccgta tttcagagcg tcgagcagga agccgaattt ctcgcgctgt tcctcttcgt
     2761 tgatacccag aataccaaac accgtctgct gcatatcacc attatggata cgtactgaac
     2821 caccgcccac ttcgtaacca ttgatgacca tatcgtaagc gttcgccacc gcattttccg
     2881 gtgcagcttt cagttctgca gccgtcatat ctttcggtga ggtgaacgga tggtgcattg
     2941 ccgtcaggcc gccttcaccg tcgtcttcaa acatcgggaa gtcgataacc cacagcggtg
     3001 cccatttgct ttcgtcggtc agaccaaggt ctttacccac tttcaggcgc agtgcaccca
     3061 tcgcgtcggc aacaattttc ttgttgtcgg caccgaagaa aatcatatcg ccatcttgcg
     3121 cggcagtacg atccaggatg tcttcgatga tttctgcatt aaggaacttc gctaccgggc
     3181 tgttgatacc ttccagacct ttcgcgcgtt cgttaacttt gatgtaagcc agacctttcg
     3241 cgccgtagat tttaacgaag ttaccgtatt cgtcgatctg cttacgggtc agcgatgcgc
     3301 cgcccggaac gcgcagagcc gctacgcgac ctttcggatc gttcgccgga cctgcaaata
     3361 cagcaaactc aacagatttc agcagatcag caacgtcagt cagttccatc gggttacgca
     3421 gatccggttt atcagaacca taacggcgtt ctgcttccgc aaaggtcatt accgggaaat
     3481 cgcccagatc cacacccttc acttccagcc acagatgacg caccagcgct tccatcactt
     3541 cacgcacttg cggcgcggtc atgaaagaag tttccacatc gatctgagta aattcaggct
     3601 gacggtcagc acgcaggtct tcgtcacgga agcatttaac gatctgatag taacggtcaa
     3661 aaccggacat catcagcagc tgtttgaaca actgcgggga ttgcggcagt gcgtagaatt
     3721 tacctttgtg tacacgagaa ggcaccaggt agtcacgcgc gccttccggc gtggctttgg
     3781 tcagcatcgg agtttcgatg tcgaggaagc cgtggtcatc cataaaacgg cgcaccaggc
     3841 tggtgatttt agcgcgggtt ttcaggcgct gagccatttc cggacgacgc aggtcgaggt
     3901 agcggtattt cagacgcgct tcttcggtgt tgacgtggtt agagtcaagc ggcagaacat
     3961 ctgcgcggtt gatgatagtc agcgaggacg ccagcacttc gatttcgccg gtcgccatat
     4021 cgcggttaat atttttttcg tcacgcgcac gtacggtgcc cgtgacctga atgcagaact
     4081 cattacgcag ttcagaggcc agctttaacg cgtccgcacg atccggatcg aaaaatacct
     4141 gcacgatacc ttcgcggtcg cgcatatcga tgaagatcag gctaccaaga tcacgacgac
     4201 ggttgaccca accacacaga gtcacctgct gccccacgtg ggacaaacgg agctgtccac
     4261 aatattctgt acgcatgaga tatcccttaa cttagctgcc ggcggatgcc ccctgctgcg
     4321 caggtgacca agtcgcagcg ttagctgtat gtcacaactg aatgaaaaaa ggcggctatt
     4381 atactggaaa ttctgccgca ccgtaagagc ctggcccgcg ctggaacgcc tcgttaccac
     4441 tttatatcgg gcctgaaatc agactctacg ccagtttgct ataaaggtgt tgcccgaact
     4501 cataaaaatt aacaaaattt gtcgttccgc catcggctaa tcgcattaag gtgtgaggca
     4561 cgattttgtt ttttcaggag tcattatgct tgaacttaat gctaaaacca ctgcgctggt
     4621 ggtgatcgat ttacaagaag gcatcttacc ttttgccggt ggtccacata ctgccgatga
     4681 ggtagttaat cgcgccggga agctggcggc gaaatttcgc gccagcggtc agcccgtgtt
     4741 tctggtgcgc gttggctggt ctgccgatta cgctgaagca ttaaaacagc cggtcgatgc
     4801 gccctcaccg gctaaagtgt tgcccgaaaa ttggtggcaa catcctgctg cattaggtgc
     4861 aaccgacagc gatatcgaaa tcatcaaacg tcaatggggt gcgttttacg gtacggatct
     4921 ggagttgcaa ttacgccgcc gaggtatcga tacaatagtg ttatgtggga tctcgaccaa
     4981 tatcggtgtt gaatccaccg cccgcaatgc ctgggaactt ggctttaatc tggtgattgc
     5041 cgaagacgcc tgtagtgccg ccagcgccga gcagcacaat aacagtatca accatatcta
     5101 cccgcgcatc gcccgtgtgc gtagcgtgga agagatcctc aacgcgttat gatttacatc
     5161 ggtctaccgc aatggtcgca tcctaaatgg gtgcggttgg ggatcaccag ccttgaagag
     5221 tatgcccgcc actttaactg cgtggagggc aacaccacgc tttacgccct gccgaaaccc
     5281 gaggttgtcc tgcgctggcg tgagcagacc acagatgact tccgcttctg ttttaagttt
     5341 ccggcgacca tttcgcatca ggcagcatta cggcattgcg atgatttagt gactgaattt
     5401 ttgacccgca tgtcaccgtt ggctccgcgc attgggcaat actggctgca actgcctgcc
     5461 acattcggcc cacgggagct gcctgcgctt tggcattttc tcgattctct tcctggcgaa
     5521 tttaattatg gcgtggaagt ccgccatcca cagtttttcg ccaaaggaga agaggaacaa
     5581 acgcttaatc gcggtttaca tcagcgcggc gttaatcggg tgattttaga cagccgcccg
     5641 gttcatgcag cacgtccaca cagtgaagct attcgcgacg ctcaacgaaa aaaacctaaa
     5701 gttccggtac atgctgtact gacggcgaca aatccgctga tccgttttat cggtagtgat
     5761 gatatgacgc aaaaccggga attatttcag gtctggttac aaaaattagc gcagtggcat
     5821 cagaccacta cgccttatct ttttttacat acgccagaca ttgcccaggc accggaactg
     5881 gtacataccc tgtgggaaga cttacgtaaa acgcttccag agatcggagc agttccggct
     5941 attccacagc aatcttctct tttctgaatt tgccacctat catagacagg tgccatcggc
     6001 cattttaaag ggagtttgta tggtaagcgc gctgtatgcc gttttaagtg cgttgttatt
     6061 aatgaagttc tcttttgatg tcgttcgcct gcgaatgcag taccgcgttg cctatggcga
     6121 cggcggtttt agcgaactgc aaagcgctat tcgcattcat ggtaacgcgg tggaatatat
     6181 tcccatcgcg attgtgctga tgctgtttat ggaaatgaat ggcgcagaaa cctggatggt
     6241 gcatatttgc ggcatcgttt tgcttgctgg tcgtctgatg cattattacg gttttcatca
     6301 ccgtctgttc cgctggcgac gttccggcat gagcgccacc tggtgtgcgc tgttgctgat
     6361 ggtgctggcg aatctttggt atatgccctg ggagttggtt ttctccctgc gttagcgcac
     6421 aatacgccac tttctttttc ccggattttt acgttatgtc tcaccgcgac acgctatttt
     6481 ctgcccctat cgccagactg ggcgactgga cctttgatga acgggtagct gaagtcttcc
     6541 cggatatgat ccagcgttcc gtacccggct attccaatat tatttccatg attggtatgt
     6601 tagccgagcg cttcgttcaa cctggtacgc aggtttacga tctgggttgt tctctgggcg
     6661 cggcgacgct ctcggtgcgt cgcaacattc atcatgataa ttgcaaaatt attgccatcg
     6721 acaactcccc ggcgatgatt gaacgctgcc gtcgtcatat tgacgcctat aaagccccta
     6781 cgccagtaga cgtcattgaa ggtgatattc gcgatatcgc cattgaaaac gcatcgatgg
     6841 tggtgctgaa ttttaccctg caattcctgg aaccttccga gcgccaggca ttactggata
     6901 aaatttatca aggactgaac ccgggcggcg cgctggtgct ttcggaaaaa ttcagtttcg
     6961 aagatgccaa agttggtgaa ctgttgttca acatgcacca cgactttaaa cgtgccaacg
     7021 gttacagcga actggagatc agccagaagc gcagcatgct ggaaaacgtg atgctgaccg
     7081 attccgtgga aacccataaa gcacgcctgc ataaagccgg ttttgagcat agtgagctgt
     7141 ggttccagtg ctttaacttt ggttcactgg tggcattaaa agcagaggac gctgcatgat
     7201 cgactttggt aacttttatt ctctgattgc caaaaatcat ctttcacact ggctcgaaac
     7261 gctgcccgcg cagattgcta actggcagcg cgagcagcag cacgggttgt ttaagcagtg
     7321 gtcaaatgcg gtggagtttc tacctgaaat taaaccgtat cgtcttgatt tattgcatag
     7381 cgttacggca gaaagcgaag agccactgag cgcagggcaa attaaacgca ttgaaacgct
     7441 gatgcgcaac ctgatgccgt ggcgcaaagg gccgttctca ctgtatggcg tcaacatcga
     7501 taccgaatgg cgttccgact ggaaatggga tcgtgttctg ccccatcttt ctgatttaac
     7561 cggacgcacc attctggatg tcggctgcgg cagcggttat cacatgtggc gcatgattgg
     7621 cgcaggcgcg cacctcgcgg tggggatcga ccctacgcaa ctttttctgt gccagtttga
     7681 agcggtgcgt aaactactgg gtaatgatca gcgcgcgcat ttgttaccgt taggtattga
     7741 acaacttccg gcactgaaag cctttgatac cgtcttttcg atgggcgtgc tttaccatcg
     7801 ccgttcaccg ctggagcatc tctggcagtt gaaagatcaa ctggtcaatg aaggtgaact
     7861 ggtgctggaa acgctggtta ttgatggtga tgagaacacg gtgctggtac caggcgatcg
     7921 ttacgcgcaa atgcgtaatg tctatttcat cccttccgcg ctggcgctga aaaactggct
     7981 gaagaagtgt ggttttgtag atatccgcat tgcagatgtg agcgttacca ctacagaaga
     8041 gcaacgacgc accgaatgga tggttactga atcgctggcc gatttcctcg acccacatga
     8101 tccgagtaag acagttgaag gttatccggc acctaaacgt gcggtgctga ttgcgcgcaa
     8161 gccgtaaagg tctggtatta ctgccggatg cggcgtgaac gccttatccg gcctacaaaa
     8221 ccttgctaat tcaatatatt gcagggacta tgtaggcctg ataagcatag cgcatcaggc
     8281 agctttacgt ttgcataacc tcagcgcccg tttccgggcg ctattcactt cttatgcctg
     8341 aatagctggc tgatcaaacg ccgttaactt cggcgcattg cccgtatatt tttcgatata
     8401 caccagcgca gagttaccgg cacaggcatt tgccagctgc gagctgggga tatccgccgt
     8461 taacacgttc gcactgccgt ttttacacaa gccattttcc agatctggcc atgcaccttc
     8521 atgcacgcat accacgcctt ttttgatccc gtcagtcacc accgcgcctg tcagaatctg
     8581 accacgtttg ttccacacac gcaccagatc accattcgca ataccaaagc gagcagcatc
     8641 ttcggtgtga atagtgattg gttcacgatc tgcaaccgca tattttttac gcagttccgc
     8701 atagttaagc tgactatgta aacggtgtgc cggatgtgcg gtcagaagct gcaactgctt
     8761 ctcgtcggcg gtacccttcc actcatcagg cgcaagccag gttgggtgtg ccgggcaatc
     8821 cttatagcca aatttttcca gcgttttgga gtaaatctca attttgccgc ttggcgtacc
     8881 cagcgcattt ttcaccggat cggcgcggaa atcaccataa cgaacgtact gttcgttctt
     8941 ctcgctgcgg cgcatttcga tcagtttatt ttgctgccag aaggcattaa acatcggcat
     9001 agtcacgcgt tgcgcacggg cacctttctg agcggcatca tagaaaaatt tcagccacgc
     9061 catttcatct ttaccttcgg tatagatctc ttttccgcca ggtttcagta attccgcaag
     9121 atcggcaaac acatcaaaat cgtttcgcgc ttcaaattgc ggagcgacag cctgcttcat
     9181 cggcacaata tgctggttgc tgtaatcacc ggtcatcgtc aagtcattgc gctcaaacga
     9241 tgtggtgatc ggtaatacga tatctgcatg tttagcagct gcggtccagt agcattcaga
     9301 aacgacgatc atctccggtt tctgccatgc tttaatcaga cgattagtat cctggtgatg
     9361 ggtaaagtta ccgccacccg cccaccagat cattttgata ttcggatacg tttgctcttt
     9421 accgttatgc tgatatttgc cgccaggatt ttccagcgca tcgacgatac gggcaacagg
     9481 aatcgccgtc attccgccat catcagcggc ctcgctggcc tgtccggcaa tcgccgcaga
     9541 catttccggc agcacgccgc caacacgtgt cgggttgcca ccattggagt aatgataaga
     9601 gaaaccaaaa ccgccgcccg gtgtaccaat ctgccctaac atcgccgcca gcgtcaccag
     9661 catccagtgt ttctgttcac catattgctg gcgctgaatt ccccagcctg ccatcagcat
     9721 tgtgcgattc gccgccatca gttcagcgag tttgactatc tgcgcttccg gtacgccagt
     9781 aatttccgcc gcccaggccg cgctcttcgg agtattatcg ctcttacctg tcagatattc
     9841 ttcaaactgc ggatacccgg tggtgtattt ttcaaggaac actttatcgt gtttgccttg
     9901 tgtcatcagg gtatgcgcaa tccctaacat cagtgccacg tcggtgccca tattcggcgc
     9961 gatccaggtg gcattatcgc caaagaactc aatggtttcg gagcggatag gatcaatggc
    10021 aatcactggt ttgccagatt ttttcagctg atggaagtat tccagccctt gctcatcggt
    10081 actgctccag gcaattttta aggtattcag cgggttcatc ccccacagca ccacaacctg
    10141 gctgttttcc agaatcagcg gccaggaggt ctgctgttca tacacctcta cagaaccgac
    10201 cacatgcggc atgatcacct gtgctgcccc ggttgaataa tcgccgctat gcccggaata
    10261 accgcccgcg aggttcatat aacgttgcag taaggtttgc gctttatgca acacgccaga
    10321 agagcgccag ccgtaagatc cggcaaaaat ggccgatggt ccgttagctt tacgaatacg
    10381 atcgtgttgc tcatgaatca gttttaatgc ttgttcccag ctcacctgta cataggtatc
    10441 ttcgcctcga cctttcgccg gttgcagtgg attatcgaga tagctttttc tcaccatcgg
    10501 atgctgaata cgcgccgtgg tgtgtacctg atccgccgcc gtagactgta aggagttcgg
    10561 tatggttttc gccagcgcgc ctgtcgaaga aacaatcttg ccgtccttca cttctacgtt
    10621 catcgctccc cagcgtcccg cggtgaggat tttaccgccc ttctcttctg cccatgcggg
    10681 tagcggtgct gccgatgtca ccaccagcgc tccagcggca ataccgctgt gtttaataaa
    10741 ttcacgtctt gttaatgtca taacttcctc cctgatcaac gaggatcact gtttctcggt
    10801 aatatctttg gcgttgtact ggaaataccg cgttaaaatg tccagttcgt tttcgctcat
    10861 gctggttcgt gcccccattc ctttggcaat ggacggccac gcattgacgg tgtaatggtc
    10921 ggcggcaata ggggcatgac aaccagcgca ataggtatcg tcaagttttt cagcgtattg
    10981 ccatagcggt ttacggtccg ctaatgcggg atcggtaagc gcaccctgta aagacgcctg
    11041 acgccattga ttgccgtatt cgtcagcctg ccattccccg tttaccgtga gcgccttgat
    11101 accttcttca cttaatgtgg ctagcgccag ccgttgacct gccgccaggt agagcgtgtt
    11161 ttcactgccc tgcatttgat aaccctgcaa cagaacgatc ggctgtttgc cactggcatc
    11221 aacgacggtg agatcggtac caggattcac ggtagccagc tcgcctatgc gagaagtttt
    11281 gaaaggataa atatgtgcgc cattagtcac tgaagtagcg gtctgacttt ccagctcatg
    11341 cgccgcgttg tcatccattt ttatttctgg cggaaaatgg gcaatgcctt tatgacaatc
    11401 gatacaggtt tcgccgccct tttgtgcttt gttatgcatt ttctgcgcag attcactttg
    11461 cgaggcaata tccatggcat caaaagaatg gcaactacgg cacgttgcag agtcagttgc
    11521 ttttaattct ttccatactg tttcggccat ttcctggcga tgagtttcga acttatcgtc
    11581 actgtctatt ttgccgctaa caaattcatg ataaatatct ttagatgctt ttaatttagc
    11641 aaataaataa tccatccctg actttggaat atggcaatcg gcacattccg cacgtatccc
    11701 tttctggttc gaaaagtgga cagttccctg atattcctca aaaggtttac tcatcgagtg
    11761 gcaggaaagg caaaatgctg tatccgacgt tttatgtaag gctttttgcg ccagcaataa
    11821 cccgccgcca ccaaccacaa ccgctatcag caaaaacaat aacccaatgc gttttttccc
    11881 tcgcatagat tcctctaaat aaattccaga acaccgaaat cattgtataa ttaaatacac
    11941 aacgaattaa ccgcttgcat aactcggcac aacactgcct gaaataatcg ataaagaata
    12001 tgatttatta caat
//
LOCUS       DAPHBJ010000135        11972 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.136, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000135 DAPHBJ010000000
VERSION     DAPHBJ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.136"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(5..1348)
                     /gene="uidB"
                     /locus_tag="RHZ76_003250"
     CDS             complement(5..1348)
                     /gene="uidB"
                     /locus_tag="RHZ76_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HDV2813842.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGLSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLN
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKV"
     gene            complement(1345..3156)
                     /gene="uidA"
                     /locus_tag="RHZ76_003251"
     CDS             complement(1345..3156)
                     /gene="uidA"
                     /locus_tag="RHZ76_003251"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDV2813843.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(3547..4137)
                     /gene="uidR"
                     /locus_tag="RHZ76_003252"
     CDS             complement(3547..4137)
                     /gene="uidR"
                     /locus_tag="RHZ76_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HDV2813844.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(4358..5125)
                     /gene="hdhA"
                     /locus_tag="RHZ76_003253"
     CDS             complement(4358..5125)
                     /gene="hdhA"
                     /locus_tag="RHZ76_003253"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HDV2813845.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASAVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(5237..6265)
                     /gene="malI"
                     /locus_tag="RHZ76_003254"
     CDS             complement(5237..6265)
                     /gene="malI"
                     /locus_tag="RHZ76_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HDV2813846.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            6440..8032
                     /gene="malX"
                     /locus_tag="RHZ76_003255"
     CDS             6440..8032
                     /gene="malX"
                     /locus_tag="RHZ76_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HDV2813847.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            8042..9214
                     /gene="malY"
                     /locus_tag="RHZ76_003256"
     CDS             8042..9214
                     /gene="malY"
                     /locus_tag="RHZ76_003256"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HDV2813848.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVVLEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRIYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            9318..10319
                     /gene="add"
                     /locus_tag="RHZ76_003257"
     CDS             9318..10319
                     /gene="add"
                     /locus_tag="RHZ76_003257"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDV2813849.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKCALREKVAAK"
     gene            complement(10353..11393)
                     /locus_tag="RHZ76_003258"
     CDS             complement(10353..11393)
                     /locus_tag="RHZ76_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDV2813850.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            11636..11761
                     /gene="blr"
                     /locus_tag="RHZ76_003259"
     CDS             11636..11761
                     /gene="blr"
                     /locus_tag="RHZ76_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HDV2813851.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
BASE COUNT         2915 a         3138 c         2916 g         3003 t
ORIGIN      
        1 gctgctacac ttttttacga ttatcaattt caaccacgat ttctttgaat tttttatccg
       61 tgagcggata aaaccagata ataacgaatg ccagtagcat aaatccgcaa ggtactaagg
      121 caattgatgt gcggatgccc ataataactt ccggcgtttg cacctgattg gcgatatatc
      181 cgcttaaccc caaaataaag gcaggaattg aacctccgat tgcctgaccg catttacggg
      241 taaatgagaa tagtgaatag gtgagccctt caattcgcac gccggtcagg tattcaccgt
      301 attctacggt atcagcttcc agcgcccaca tcacggtcat ggtaacgccc tgaccaattg
      361 aagcgatggc caacgcaacc aacgccaccg gcagtgacca gacggaaacc cagaagaaca
      421 gcagataacc gcaggttccc agcaaagcgc caatcaggaa ggtattcttt ttaccgatcc
      481 tcgcgaccat ccccggcacc agcggtgccg atgccacagt accaaccagg ttttgcacca
      541 gtaccagcac agtgaacagc ccggtatcat ttaacacata gcgcacgtag aacaacgacg
      601 aggcgctgac cgcaaaggtc gaaatcagca cacacagcgc accgatgcac aacataaaca
      661 gcgggcgatt ccgtttcagg gtttgcagac tgatattcaa tgacggctgc gcaacgatac
      721 gtaccacatt ctcacgcgtc gatttgaagc agatgaagta aagcaccatt ccggcaatcg
      781 ccagcacaat tgtccagaaa tggtataccg acaccatctc ttccgggctg gagttcttaa
      841 tgctcagtcc tatcagaaat gccaggcaga caaaggtcaa tgaagcggca atcccacgag
      901 ccgcgcccag acgggcgcgg gattgtggtt gttgggtcat cgcggtagca agtgaaccat
      961 aaggaatatt caccaggctg tagcaaagcc cgaggcccat gtaggtcaaa tatgcataca
     1021 ccactttgct accatggctc cagtcggtcg gcacccagaa taccagcacg ctgaagatca
     1081 ttaacggcgc agtaccgaag agtaaaaacg ggcggaattt tccccagcgg gtattcacac
     1141 tgtccaccac tcgtccggca aagacgtcgg cgaaggcatc gaatacccgc accagtaaca
     1201 gcatggtgcc cgccgcagcg gcaccgacgc cagcgacgtc ggtgtagtaa ctcaacagga
     1261 agagcgcccc cattgcgaag gcgaagttat tggcgacgtc accgaggctg tagccgacga
     1321 tggtgcgcca ggagagttgt tgattcattg tttgcctccc tgctgcggtt tttcaccgaa
     1381 gttcatgcca gtccagcgtt tttgcagcag aaaagccgcc gacttcggtt tgcggtcgcg
     1441 agtgaagatc cctttcttgt taccgccaac gcgcaatatg ccttgcgagg tcgcaaaatc
     1501 ggcgaaattc catacctgtt caccgacgac ggcgctgacg cgatcaaaga cgcggtgata
     1561 catatccagc catgcacact gatactcttc actccacatg tcggtgtaca ttgagtgcag
     1621 cccggctaac gtatccacgc cgtattcggt gatgataatc ggctgatgca gtttctcctg
     1681 ccaggccaga agttcttttt ccagtacctt ctctgccgtt tccaaatcgc cgctttggac
     1741 ataccatccg taataacggt tcaggcacag cacatcaaag agatcgctga tggtatcggt
     1801 gtgagcgtcg cagaacatta cattgacgca ggtgatcgga cgcgtcgggt cgagtttacg
     1861 cgttgcttcc gccagtggcg cgaaatattc ccgtgcacct tgcggacggg tatccggttc
     1921 gttggcaata ctccacatca ccacgcttgg gtggtttttg tcacgcgcta tcagctcttt
     1981 aatcgcctgt aagtgcgctt gctgagtttc cccgttgact gcctcttcgc tgtacagttc
     2041 tttcggcttg ttgcccgctt cgaaaccaat gcctaaagag aggttaaagc cgacagcagc
     2101 agtttcatca atcaccacga tgccatgttc atctgcccag tcgagcatct cttcagcgta
     2161 agggtaatgc gaggtacggt aggagttggc cccaatccag tccattaatg cgtggtcgtg
     2221 caccatcagc acgttatcga atcctttgcc acgcaagtcc gcatcttcat gacgaccaaa
     2281 gccagtaaag tagaacggtt tgtggttaat caggaactgt tcgcccttca ctgccactga
     2341 ccggatgccg acgcgaagcg ggtagatatc acactctgtc tggcttttgg ctgtgacgca
     2401 cagttcatag agataacctt cacccggttg ccagaggtgc ggattcacca cttgcaaagt
     2461 cccgctagtg ccttgtccag ttgcaaccac ctgttgatcc gcatcacgca gttcaacgct
     2521 gacatcacca ttggccacca cctgccagtc aacagacgcg tggttacagt cttgcgcgac
     2581 atgcgtcacc acggtgatat cgtccaccca ggtgttcggc gtggtgtaga gcattacgct
     2641 gcgatggatc ccggcatagt taaagaaatc atggaagtaa gactgctttt tcttgccgtt
     2701 ttcgtcggta atcaccattc ccggcgggat agtctgccag ttcagttcgt tgttcacaca
     2761 aacggtgata cgtacacttt tcccggcaat aacatacggc gtgacatcgg cttcaaatgg
     2821 cgtatagccg ccctgatgct ccatcacttc ctgattattg acccacactt tgccgtaatg
     2881 agtgaccgca tcgaaacgca gcacgatacg ctggcctgcc caacctttcg gtataaagac
     2941 ttcgcgctga taccagacgt tgcccgcata attacgaata tctgcatcgg cgaactgatc
     3001 gttaaaactg cctggcacag caattgcccg gctttcttgt aacgcgcttt cccaccaacg
     3061 ctgatcaatt ccacagtttt cgcgatccag actgaatgcc cacaggccgt cgagtttttt
     3121 gatttcacgg gttggggttt ctacaggacg taacataagg gactcctcat taagataata
     3181 atactggtca acctttaatc tgattagtta accaattctg tgttgcacac agttatctgt
     3241 gagaaaccca tggaagagaa atggaaataa aaagaactga caccgcgatc aaaaaaccaa
     3301 aaactgcaat acccacagcg tgatatagat cgcattaatc tttaaaacag cgttgacaga
     3361 agccataaaa taagcaaccg aacgttcgtt cgagcgtatt gggaacgata aactctcatg
     3421 acatatggct acagtgaata ttttgggggg aattctccga agccgggaag gataattttt
     3481 gcacagagaa attacgatat gaaacgcggg cacagttatc accagcccgc cgaaaaagag
     3541 agagaatcag gatgcggtta agataccgcc aatcatcgcg cgtaatccct gagcaaacga
     3601 cgcttcacgc gccaggtctt ccgcttcgat atcggccagt acaccgtagg tcagatccag
     3661 cagtaaacgt gaagtcattg ccgtgttgat gtctgggctt atctcgcctt tttgctgggc
     3721 atcacgcatc cgctgggcaa caaattccgt gatcgtcata tgcttatttt taagcatggc
     3781 ggcgacctgt gggttacgca tcccttccgc cataatttca accaccagcg cccgttgtcc
     3841 aaaggcttca tgggtgagag agacaatgga ctcgaccata tagtcaacga aatgaatccc
     3901 ttcaatcggt tcccggaaac gggccagcgc cctctcctgg tcctgtaaga taatcgcctg
     3961 aatcaaggct tctttggaga tgaaatggtg atagagcgtc ccgggactaa tggcgcaaga
     4021 tttacagatg gctttcatcg aggcactgtg aaatccattt tctgaaaaaa tctctctggc
     4081 agcattgagg atccgggtcc gtgtcggttg tgcttcagtc tgcatgttat ccatcattaa
     4141 accattatag ttagcgtgaa taactagttt accgcgttgt caggtgagcg cctatcattg
     4201 ctgttgaata gtgcgaaggc acactctatt cgtatacttt tcactctgaa attggttaac
     4261 caatagccgt gatttggctg ccagttattt agcagagacg ttgatgagtt tatcccggta
     4321 aacaacacgc acggttgttt accggtccgt tagtgtatta attgagctcc tgtaccccac
     4381 caccggagac ggtgagaatt tgtccgctta cccagctcgc agcaggcgag caaaggaaca
     4441 gcgctgcgtt agcaatatct tgcggttggc ccagacgtct gatcggcgtg tgctgtaaca
     4501 ttttttgttc aatttctggt gtaataacgg atttcagggc atcggttaat attgcccccg
     4561 gcgcaatgcc atttacccga atattttttt cacccaggtc aaacgccata tttctgacca
     4621 gatgactggc cgcagcttta gatgatgcat aggaagtcat gtttatattt ttattttctg
     4681 ccgccataga agtgatggtc agaataacgc caccgccatt tttttccatt tctggcgcaa
     4741 caagttgtga cagatggaaa aaagaaaaca cattcagttc ataagcacgg cgaaaatccg
     4801 ccattggcat atcaaacggt ttaggtccac cgccaccggc gttgttaacc agaatatcaa
     4861 ctttacccag cttactgata gcaaagtctg ccagtgcaga gagttcctgt tcggaagtaa
     4921 tatcacaacg gcaggcaaat gcctgaccac ccagttgttg aatttcgtct acaacatggt
     4981 tagctgcgtc ggcgttaata tcactgacca ccgcagatgc gccagctgtc gcgaatgtaa
     5041 tggcgatttc tttaccaata cctgcacccg cacctgtgat gatggcgcat tttccgtcga
     5101 gtctcaggtt gtcagaatta aacacataaa cctcctgtga acttcagtaa aaataaaagg
     5161 caccgcctgt gtgccacatt ttttatcatt aaccttgagg aaaggacact accaggtata
     5221 gcagataaag agaggattat ttcgctgcaa tgagccgggc gggaataata agattgcgtg
     5281 aatgcgtctc ttcatgggtg attttttgca tcatgcgatc cgcaagtgtg ataccaagtt
     5341 cccgcgctgg cgtgctggcc caggtaacag gtatatcatc aagtgtggtt ggtgtcgcat
     5401 cggtaaatgc cgccagcgaa acctgttgct caaagtaacg atcgactccg ctttccccgc
     5461 tttgccgccc tgctttgagc aaaccaaacc atgcccccat cgcaatagtt tcgttatagc
     5521 aaaccacggc actgatggtc gggttatgac gtaaaagcgc cgtgatagct tccgcggctt
     5581 gcttctggct ggaagtgcac tccaacaccc aatcgctatg aaacggcagg ccaaatttta
     5641 gtagagttgc acaatagccc ccaacccgtt ctgcacgggt taatgaggaa ctttgccctc
     5701 ccagccaggc gatccgctga tgcccattgc gaatgagatg ctccgtcaac aactgtgcag
     5761 cctgcatgtt gtccgggcga accgtatcaa catcatcaag ataactggca cgggaagcga
     5821 aaatcaccgg gatagctttt tcttctgcca tccgtcgcag gtcatcgcta cttcctgcag
     5881 ctccggcaat taccacacca tcgacaccct gattcagtaa cagtgaaaac cgctgtgcca
     5941 gctgctcacc gtctttaccg ccgtgaagca aaaaaaccat ccgtccctgc gcttccagag
     6001 cttccgtcaa tccggccgtc aattcggcgt aaaacggcgc agataaatca cggacgatca
     6061 aaccaatgac gccgctttgc ccgccgcgca gcgccgacgc ctggcgattg cgcacaaatc
     6121 ccagctcttc aatggcggcg ttaacgcgtt ctcctgtggc ggtagagatt cgccctttgc
     6181 cactaagcac cagcgaaacg gtacttaccg acacgcccgc agccagcgca acatcatgaa
     6241 tggttatttt tttggcggta gccatgcaga tgacctactc cctgattatg tgacagataa
     6301 aacgttttac cttttattta tcttataccc gctattatcg ttgcgtaatg tgatttatgc
     6361 ctcactaaaa tttgataaaa cgttttatct tctcgcgcaa tttactgaat ccagattgtt
     6421 ctctacgagg agtcgtttta tgacggcgaa aacagcaccg aaagtcacgc tgtgggagtt
     6481 cttccagcag ttaggcaaaa ccttcatgtt acccgtggca ttattgtcgt tctgcggcat
     6541 tatgctcggc attggtagtt ctcttagcag ccatgatgtc ataaccctga tcccggtcct
     6601 gggcaacccc gtgttgcagg ctatctttac ctggatgagt aagattggct cgtttgcttt
     6661 tagtttcctg cctgtcatgt tctgtatcgc catcccgctg ggcctggcac gcgaaaataa
     6721 aggcgtagcg gcattcgctg gcttcgtcgg ttatgcggta atgaacctcg cggtaaactt
     6781 ctggttgacc aataaaggca ttctgccaac cacggatgcc gcggttctga aagccaataa
     6841 catccagagc attcttggga tccagtcgat cgataccggg atcctcggtg cggtgatcgc
     6901 cggtattatc gtctggatgc tgcatgagcg tttccataat atccgcctgc cggatgcgct
     6961 ggcattcttc ggcggtacgc gcttcgtacc aattatctcc tcgctggtga tgggccttgt
     7021 cggcctggtg attccattag tctggccgat tttcgccatg ggtattagcg gcttgggcca
     7081 tatgataaac agcgcgggtg atttcggacc gatgctgttt ggtaccggtg aacgtctgct
     7141 gttgccgttt ggtctgcatc acattctggt ggcattaatt cgctttaccg acgcaggcgg
     7201 cacgcaggaa gtctgcggtc aaaccgtcag cggcgcactg accatcttcc aggcgcaatt
     7261 gagttgcccg accactcacg gtttttctga aagcgccacg cgtttccttt cgcaaggtaa
     7321 aatgcctgcg tttctcggcg gtctgccagg tgcagcgtta gctatgtatc actgcgcgcg
     7381 cccggaaaat cgccataaaa ttaaaggtct gctgatttct ggcctgatcg cctgcgtcgt
     7441 tggcggcact accgaaccgc tggaattcct gttcctgttc gtagcgccag ttctgtatgt
     7501 catccacgcg ctgttaaccg gcctcggctt caccgtcatg tctgtgctcg gcgtcaccat
     7561 cggtaatacc gacggcaata tcatcgactt cgtggtgttc ggtattttgc atggtctgtc
     7621 aaccaagtgg tacatggtgc cagtggtggc ggcaatctgg tttgtcgttt actacgtcat
     7681 cttccgtttc gctatcaccc gcttcaatct gaaaaccccg gggcgcgata gcgaagttgc
     7741 cagctcaatc gaaaaagccg ttgccggtgc gccgggtaaa tcaggttaca acgttcctgc
     7801 aatcctcgaa gcattaggcg gtgccgacaa tattgtcagc ctcgataact gcattacccg
     7861 tctgcgtttg tctgtgaaag atatgtcgct tgttaatgtg caggcactga aggacaatcg
     7921 ggcaattggc gtagtacaac ttaatcaaca taacctgcag gttgttatcg ggccacaagt
     7981 tcagtcagta aaagatgaaa tggccggtct gatgcatact gtccaggcat aaggataaga
     8041 tatgttcgat ttttcaaagg tcgtggatcg tcatggcaca tggtgtacac agtgggatta
     8101 tgtcgctgac cgtttcggca ctgctgacct gttaccgttc acgatttcag acatggattt
     8161 tgccactgcc ccctgcatta tcgaggcgct gaatcagcgc ctgatgcacg gcgtatttgg
     8221 ctacagccgc tggaaaaacg atgagtttct cgcggctatt gcccactggt tttccaccca
     8281 gcattacacc gccatcgatt ctcagacggt ggtgtatggc ccttctgtca tctatatggt
     8341 ttcagaactg attcgtcagt ggtctgaaac aggtgaaggc gtggtgatcc acacacccgc
     8401 ctatgacgca ttttacaagg ccattgaagg taaccagcgc acagtaatgc ccgttgtttt
     8461 agagaagcag gctgatggtt ggttttgcga tatgggcaag ttggaagccg tgttggcgaa
     8521 accagaatgt aaaattatgc tcctgtgtag cccacagaat cctaccggga aagtgtggac
     8581 gtgcgatgag ctggagatca tggctgacct gtgcgagcgt catggtgtgc gggttatttc
     8641 cgatgaaatc catatggata tggtttgggg cgagcagccg catattccct ggagtaatgt
     8701 ggctcgcgga gactgggcgt tgctaacgtc gggctcgaaa agtttcaata ttcccgccct
     8761 gaccggtgct tacgggatta tagaaaatag cagtagccgc gatgcctatt tatcggcact
     8821 gaaaggccgt gatgggcttt cttccccttc ggtactggcg ttaactgccc atatcgccgc
     8881 ctatcagcaa ggcgcgccgt ggctggatgc cttacgcatc tatctgaaag ataacctgac
     8941 gtatatcgca gataaaatga acgccgcgtt tcctgaactc aactggcaga tcccacaatc
     9001 cacttatctg gcatggcttg atttacgtcc gttgaatatt gacgacaacg cgttgcaaaa
     9061 agcacttatc gaacaagaaa aagtcgcgat catgccgggg tatacctacg gtgaagaagg
     9121 tcgtggtttt gtccgtctca atgccggctg cccacgttcg aaactggaaa aaggtgtggc
     9181 tggattaatt aacgccatcc gcgctgttcg ttaaccccaa ttgcgcaacg taaaaaatcg
     9241 ttgcgcaatc gtggattttt accctgcttt gtttttataa tggtgcgcac ttttatatcc
     9301 agaaaaagag tgcgaccatg attgatacca ccctgccatt aactgatatc catcgccacc
     9361 ttgatggcaa cattcgtccc cagaccattc ttgaacttgg ccgccagtat aatatctcgc
     9421 ttcctgcaca atccctggaa acactgattc cccacgttca ggtcattgcc aacgaacccg
     9481 atctggtgag ctttctgacc aaacttgact ggggcgttaa agttctcgcc tctcttgatg
     9541 cctgtcgccg cgtggcattt gaaaacattg aagatgcagc ccgtcacggc ctgcactatg
     9601 tcgagctgcg tttttcacca ggctacatgg caatggcaca tcagctgcct gtagcgggtg
     9661 ttgtcgaagc ggtgatcgat ggcgtacgtg aaggttgccg cacctttggt gtgcaggcga
     9721 agcttatcgg cattatgagc cggaccttcg gcgaagccgc ctgtcagcaa gagctggagg
     9781 cctttttagc ccaccgtgac cagattaccg cacttgattt agccggtgat gaacttggtt
     9841 tcccgggaag tctgttcctt tctcacttca accgcgcgcg tgatgcgggc tggcatatta
     9901 ccgtccatgc aggcgaagct gccgggccgg aaagcatctg gcaggcgatt cgtgaactgg
     9961 gtgcggagcg tattggacat ggcgtaaaag ccattgaaga tcgggcgctg atggattttc
    10021 tcgccgagca acaaattggt attgaatcct gtctgacctc caatattcag accagcaccg
    10081 tagcagagct ggctgcacat ccgctgaaaa cgttccttga gcatggcatt cgtgccagca
    10141 ttaacactga cgatcccggc gtacagggag tggatatcat tcacgaatat accgttgccg
    10201 cgccagctgc tgggttatcc cgcgagcaaa tccgccaggc acagattaat ggtctggaaa
    10261 tggctttcct cagcgctgag gaaaaatgcg cactgcgaga aaaagtcgcc gcgaagtaac
    10321 aaaatggatg gtgcaaatgc accatccatt tttcatgcaa ggcacaaagt cgcgcgatgt
    10381 ttggcggatt cgatgcccag ctcaatcaac tccattacct ggattgcctg gcttgccgga
    10441 accggatttt caccatcgcc atttaacgca tcacgaatag ccgcatagta agccggataa
    10501 ttcccaggca ccgtcaacag cgtttcttcg acacgttcct caccttccac gcgggtaagt
    10561 acgccatcac gcatatcgta gccccagtct tcctgcggta gacgctcgcc atttttcaga
    10621 cgttcttcct gtggatcgag gccatatttc acataactgc ctcgggatcc atgcacgata
    10681 taccgtgctg actcagcggc tgccagcatg gtaccgtgta aaatgactcg ccgctgtgga
    10741 taggacaaga tggcgtggaa ataatcggtc gactgcgctc cgggccgtaa ctgtgccaaa
    10801 tctaccgtca tgctgaccgg taaaccaaaa agcgtaatgg cctgatcaag aagatgtggt
    10861 gctaaatcgt accagatacc gctgcctggg ccgccctgtt cacgccaacg atcgcgcacc
    10921 tgcggacgga agcggtcaaa atgagactca aagtaagcaa cttcacccag cacgccttcc
    10981 gcgagtaaac cttttagcgt caagaaatcg ctatcccagc gacggttatg gaatacagac
    11041 agcacacgcc ccaggctttt tgccagcgca tccagctctc gcgcttgtga cagtgtcacg
    11101 gtaaagggtt tatcaacgac cacatgttta cccgcctcaa gcgccgcttt ggctaacggg
    11161 aaatgggtat cgttgggtgt aggaatgaca atcaggtcta tgttgggatc gttaaacaga
    11221 tgcttcggct cagagacaac cgtaaccgtt ggccagtcgg cttttacttt tgtttcatca
    11281 ctgctggaga ttaccgccag ttccagcccg ggcgtgcccg caatcagggg cgcatggaag
    11341 gttttgctcg cataaccata cccaatcaac ccaacacgga tgttgtcgct catgtcattg
    11401 cctctcattg aagtatgatg gctatttgac actatccttt acccacgctc aacagtttaa
    11461 taacctgcca gcaataaggg atgttgttta acttaagtca aaaaaatagc gaattttcca
    11521 acgacaaaag ctaaatatcg caaaaacctc agtaaaaatc ttgctggagc tattattgct
    11581 aagtaacatt taccccctga agttaatgga tcaatcaaga gagatgtggg ctgtaatgaa
    11641 tcgtcttatt gaattaacag gttggatcgt tcttgtcgtt tcagtcattc ttcttggcgt
    11701 ggcgagtcac attgacaact atcagccacc tgaacagagt gcttcggtac aacacaagta
    11761 agctctgcac ttgtggagcg acatgctgcc cgtccgggcg catgttttca cttgtcggat
    11821 attaaaccag gaatttatta tcttgttcga tgttgttggt gattgtcagg gatagtaaag
    11881 cgcgttactc ttttcagagc agtttttcat ccctgaatat ttgccatcag cgcattctta
    11941 ttattgtaat aacacaagcg ttcacttttg gt
//
LOCUS       DAPHBJ010000136        11941 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.137, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000136 DAPHBJ010000000
VERSION     DAPHBJ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.137"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            37..1107
                     /gene="ygjJ"
                     /locus_tag="RHZ76_003260"
     CDS             37..1107
                     /gene="ygjJ"
                     /locus_tag="RHZ76_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HDV2813852.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            1124..3475
                     /gene="ygjK"
                     /locus_tag="RHZ76_003261"
     CDS             1124..3475
                     /gene="ygjK"
                     /locus_tag="RHZ76_003261"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDV2813853.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            3801..5819
                     /gene="fadH"
                     /locus_tag="RHZ76_003262"
     CDS             3801..5819
                     /gene="fadH"
                     /locus_tag="RHZ76_003262"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HDV2813854.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDISLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(5864..6280)
                     /gene="higA"
                     /locus_tag="RHZ76_003263"
     CDS             complement(5864..6280)
                     /gene="higA"
                     /locus_tag="RHZ76_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HDV2813855.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNA
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(6277..6591)
                     /gene="higB"
                     /locus_tag="RHZ76_003264"
     CDS             complement(6277..6591)
                     /gene="higB"
                     /locus_tag="RHZ76_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HDV2813856.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(6876..8012)
                     /gene="rlmG"
                     /locus_tag="RHZ76_003265"
     CDS             complement(6876..8012)
                     /gene="rlmG"
                     /locus_tag="RHZ76_003265"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HDV2813857.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR"
     gene            8097..8600
                     /locus_tag="RHZ76_003266"
     CDS             8097..8600
                     /locus_tag="RHZ76_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDV2813858.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            8677..9360
                     /locus_tag="RHZ76_003267"
     CDS             8677..9360
                     /locus_tag="RHZ76_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HDV2813859.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKDVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAQK"
     gene            9439..10425
                     /locus_tag="RHZ76_003268"
     CDS             9439..10425
                     /locus_tag="RHZ76_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV2813860.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKINVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAQGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            10708..11673
                     /gene="alx"
                     /locus_tag="RHZ76_003269"
     CDS             10708..11673
                     /gene="alx"
                     /locus_tag="RHZ76_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HDV2813861.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPTALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         2822 a         3102 c         3227 g         2790 t
ORIGIN      
        1 aggctgtact ttttctttag ctcatggatt aacacaatga aattaatcac tgcaccatgc
       61 agagcattac ttgctctgcc gttttgctac gccttttctg cggcaggaga agaagcacgt
      121 ccggcagaac atgacgacac aaaaacacct gcaattacct cgacatcttc tccttcattt
      181 cgtttttacg gcgaattagg ggttggtgga tatatggatt tagagggtga gaataaacat
      241 aaatacagcg acggcaccta tattgaaggt ggcctggaga tgaagtacgg ctcctggttc
      301 ggcctgattt acggcgaagg ctggaccgtg caggccgacc acgacggcaa tgcctgggtg
      361 ccagaccata gctggggtgg tttcgaggga ggaattaacc gtttctatgg cggttatcgt
      421 accaatgatg gcaccgaaat catgctcagt ctgcgtcagg attcctcgct ggatgacctg
      481 caatggtggg gcgacttcac ccccgatctg ggctacgtca ttcccaatac ccgcgacatt
      541 atgaccgcgc tgaaggtgca gaacttaagc ggcaactttc gttatagcgt caccgccaca
      601 cctgccggac atcatgacga aagcaaagcc tggctacatt ttggcaagta cgatcgctat
      661 gacgacaaat acacctatcc ggcaatgatg aacggttaca tccagtatga ccttgccgaa
      721 ggcatcacct ggatgaacgg tctggaaatc accgacggca caggacagct ctatctcacg
      781 ggcctgctaa ctcctaactt tgccgctcgc gcctggcacc ataccggacg cgccgacggg
      841 ctggacgtac cgggaagtga aagtgggatg atggtgagcg ccatgtatga agcgttaaag
      901 ggcgtttatc tctccaccgc ttacacctac gccaaacatc gccctgacca cgctgacgat
      961 gaaaccacct ctttcatgca gtttggtatc tggtacgaat acggcggcgg acgtttcgcc
     1021 acggcttttg atagccgctt ctacatgaaa aatgcctctc acgatcccag cgaccaaatc
     1081 ttcctgatgc aatatttcta ctggtaataa ggactgtaat ttcatgaaaa taaaaactat
     1141 tttaacgcca gtaacctgcg ctctgctgat aagtttttcc gcccatgccg ctaacgccga
     1201 caattataaa aacgtgatta accgtactgg cgcgccgcag tacatgaagg attacgatta
     1261 cgacgatcac cagcgtttta atcctttttt cgatctcgga gcctggcatg gtcatctgtt
     1321 gccagacggc cctaacacca tgggcggctt tccgggcgtt gcgctgctga cggaagagta
     1381 catcaacttt atggccagca atttcgaccg cctgaccgtc tggcaggacg gcaagaaagt
     1441 cgactttacg ctggaggcat acagtattcc cggtgcgctg gtgcaaaaac tgacagcaaa
     1501 agatgtgcaa gtcgaaatga ctctgcgctt cgccacgccg cgcacgtcac tactggaaac
     1561 caaaatcacc agcaataaac cgctggatct ggtgtgggac ggcgaactgc tggaaaaact
     1621 ggaagcgaaa gaagggaaac cgctttccga taaaaccatt gctggcgaat accctgacta
     1681 tcagcgcaaa atcagcgcca cccgtgatgg cctgaaagtc acctttggca aagtgcgcgc
     1741 cacctgggat ctgctgacct ccggcgaatc ggaatatcag gtgcataaat ccctgccggt
     1801 gcagactgaa atcaacggta atcgctttac cagtaaggcg catatcaacg gttcgacgac
     1861 gctctatacc acctattccc atctgctgac cgctcaggaa gttagcaaag agcaaatgca
     1921 gatccgcgat attctggcgc gtccggcgtt ttatctcacc gcctcgcagc aacgctggga
     1981 agagtatctg aagaaagggt taaccaatcc ggatgcgacg ccggaacaga cgcgcgtcgc
     2041 ggtgaaagcg atcgaaacgc tcaacggtaa ctggcgctcg cctggcggcg cggtgaaata
     2101 caacaccgtc acgccgtcgg tgaccgggcg ctggttctcc ggcaatcaga cctggccgtg
     2161 ggatacctgg aagcaggcgt ttgcgatggc gcatttcaat ccggacatcg ccaaagagaa
     2221 tatccgcgcg gtcttctcct ggcagatcca gcccggcgac agcgtgcgtc cgcaggatgt
     2281 gggctttgtc cccgacctga tagcgtggaa ccttagcccc gagcgtggtg gcgatggcgg
     2341 caactggaac gaacgtaata ccaaacccag ccttgccgcc tggtcggtga tggaagtgta
     2401 taacgtcacc caggataaaa cctggctggc agagatgtac ccgaaactgg tggcctatca
     2461 cgactggtgg ttacgtaacc gcgatcacaa cggcaacggc gtgccggaat atggcgcgac
     2521 ccgcgacaaa gcccacaaca ctgagagcgg cgagatgctg tttacggtga aaaaaggcga
     2581 taaagaagag acgcagtctg ggctgaacaa ctacgcccgc gtggtggaga aaggccagta
     2641 cgacagtctg gaaattccgg cacaggttgc tgcatcgtgg gaatcgggcc gtgatgacgc
     2701 cgccgtcttt ggttttatcg acaaagaaca gttggataaa tatgtcgcta atggcggcaa
     2761 acgtagcgac tggacggtga aattcgccga aaaccgcagt caggacggaa cgttgctggg
     2821 ctactcgcta ttgcaggagt cggtggatca ggccagctat atgtacagcg ataaccatta
     2881 tctggcggag atggcgacca tcctcggtaa gccagaagag gccaaacgct atcgccagtt
     2941 ggcacagcag ctcgcggact acatcaacac ctgtatgttc gacccgacta cccagttcta
     3001 ctatgacgta cgtattgaag ataaaccact ggcgaacggc tgcgcgggca aaccgattgt
     3061 tgagcgcggt aaagggccgg aaggctggtc gccgctgttt aacggtgcgg caacgcaggc
     3121 caatgccgac gcggtggtga aggtgatgct cgatcctaaa gagttcaaca cctttgtccc
     3181 gctgggaacg gcggcgttaa ccaatccggc ttttggcgct gatatctact ggcgtgggcg
     3241 cgtatgggtg gatcagttct ggtttggtct gaaagggatg gagcgttacg gttatcgtga
     3301 tgatgccctg aagctggcgg atacgttctt ccggcacgcc aaagggttaa ccgccgatgg
     3361 accaattcag gaaaattaca acccgctgac aggcgcacag caaggcgcac caaatttctc
     3421 ctggagtgcc gcgcatttgt atatgttgta taacgatttt ttccgtaagc aataatttgc
     3481 aactgctggc ggatgcggcg taaacgcctt atccgcccta catgtgcaat cctcctggcc
     3541 ggataaaacg cggcaagcgt tgcatccggc aactgcaccg cgccactggc ggatgcggcg
     3601 tgaacgcctt atccgcccta catgtgcaat cccccaggcc ggataaaaca cggcaagcgt
     3661 cgcatccggc gttatcaccg ggcgtattct ttttgaatcc catcacaaac cccgcactcc
     3721 ccttttccct tttctccggc gacggctaaa ttagaactca tccgaccaca taacaattat
     3781 tttacatact ggacaatttt atgagctacc cgtcgctgtt cgccccgctg gatttaggtt
     3841 ttaccacgtt aaaaaaccgc gtgttgatgg gctcaatgca caccgggctg gaggaatacc
     3901 cggacggtgc cgagcggctg gcagcgtttt atgccgaacg cgcccgtcac ggcgtggcgc
     3961 tgattgtcag cggcggcatt gcaccagatt taacaggcgt tggcatggaa ggtggcgcaa
     4021 tgctcaacga cgccagccag atcccacacc atcgcaccat taccgaagcg gtacatcagg
     4081 aaggcggcaa aatagccctg caaattttgc ataccgggcg ctacagctac caaccgcatc
     4141 tggtcgctcc ctccgcattg caggccccca tcaaccgttt cgttcctcac gaactcaccc
     4201 atgaagagat cctgcaactg atcgacgatt tcgcccactg cgcgcaactg gcacgggagg
     4261 caggatacga cggtgtagag gtaatggggt ccgaagggta tttgatcaac gaatttctga
     4321 cgctgcgcac caatcagcgt agtgaccagt ggggcggcga ttaccgcaac cggatgcgat
     4381 ttgccgtaga agtagtgcgt gcggtgcgcg aacgcgtcgg caacgacttc attattatct
     4441 accgactgtc gatgctcgac ctggtcgaag acggcgggac ttttgccgaa acggtagagc
     4501 tggcgcaggc cattgaagcg gcgggcgcga ccattatcaa caccggcatt ggctggcatg
     4561 aagcacgtat tccgaccatt gccacgcccg tgccgcgcgg cgcatttagc tgggtcacgc
     4621 gcaaactgaa aggccacgtc tcgctgccgc tggtaaccac caaccggatt aacgatccgc
     4681 aggttgccga cgatattctc tcgcgcggcg atgccgatat ggtatcgatg gcacgaccgt
     4741 ttcttgctga tgcggaactg ctgtcaaaag cgcaatcggg gcgagccgat gagatcaaca
     4801 cctgcattgg ctgcaatcag gcctgcctcg atcagatctt cgttggcaaa gtcacctcgt
     4861 gcctggtgaa tcctcgcgcc tgccacgaaa ccaaaatgcc aatccttcct gccgtgcaga
     4921 aaaaaaatct ggcggtggtc ggtgcgggac ctgctgggct ggcgtttgcc attaacgcgg
     4981 cggcgcgtgg gcatcaggta acattgtttg atgcccacag cgagatcggc gggcagttta
     5041 atatcgccaa acagatcccc ggcaaagagg agttttacga aacgctgcgc tattaccacc
     5101 ggatgatcga agtgacgggc gtgacgctga aactcaatca caccgtgacg gcggatcagt
     5161 tacaggcgtt cgatgaaacg atcctcgcca gtgggatcgt gccgcgcgtt ccgcctatcg
     5221 acgggatcga tcatccgaag gtattgagtt atctcgatgt actgcgcgac aaagcgccgg
     5281 taggcaacaa ggttgccatc atcggttgtg gcgggattgg ttttgatacg gcgatgtatt
     5341 taagtcagcc gggcgaatcc accagccaga atatcgccgg gttctgtaat gagtggggga
     5401 tcgacatcag cctacaacag gctggtggct taagcccgca gggaatgcag atcccgcgta
     5461 gcccacggca aattgtgatg ctccagcgca aagccagcaa accaggacag gggttaggca
     5521 aaaccaccgg ctggatccat cgcaccaccc tgctctcgcg gggtgtgaaa atgatcccag
     5581 gcgtaagtta tcagaagatt gacgatgacg ggctgcatgt ggtgatcaac ggcgaaacgc
     5641 aggtattagc agtggacaat gtggtgatct gcgcagggca agagccaaac cgcgcgctgg
     5701 cgcaaccgct gattgatagc gggaaaactg tgcatttaat tggcggctgc gatgtggcta
     5761 tggagctgga cgcacgacgg gcaattgccc agggaacacg gctggcgctg gagatttaaa
     5821 tcgttatttg aagcgccgga tgcaacgcat ccggcacgtc gcgttaatca ataaacaagg
     5881 cgggagaaat gccgaatcgc gttgccaatt ttttagcgtg ttccagcgtt aatttcctct
     5941 tcccgctcaa aacgcgtgac accatagatt tactgccaat ttccggcaga tcggaaaggg
     6001 ttaaaccata ttgatccata agggtacgaa tcacggctat accgccaggc atggcttgag
     6061 ccatggcatt aaattccgca aattcgggcg ctgattcttc ccacgcggtt attttggcac
     6121 acaccagatc cagcaagggg ttttcaggag cgttgagcag cagatgatct accagttcca
     6181 gcgcctgggt gtattgttcc tcgttctgaa tacccgccag aaaaggtgcc accgcagtta
     6241 gcttttctcc tgcttgcaag atgtcggcaa tcgcaatcat tttttcccct tagtacgatg
     6301 aacagcggta aagaaatcgt attctttatg cgtcataact tcacgtatgt agcacttttg
     6361 cgattcaaaa aagaccattg ctacaacacg taattcattg cccccaacat tgaaaacata
     6421 atgcttatcc agatatttga agttatccag agatgggaat actgctttta atgactcagg
     6481 ttttttgaaa tatcccttag caatcgtgtt ccctagagcc accaactccg ttttatgttg
     6541 cgggtatttt tccgcagcat ctttcaatgc tttttgagtt atcaggtgca ttcttcatca
     6601 cgtccgttga caaattggca atatgataac atccgttgac agattggcaa cggatgaatt
     6661 tatctgtgtg acggaagaat aaacgtagaa aaacgctaaa caagagaatg tagaggatgg
     6721 tgagagggat tttttgtttt atggaagcgc gattccagaa ctgggcagac attgccggat
     6781 gcgacgcttg ccgcgtctta tccggcctgg gagtaacgtg ctgaacgtag gccggataag
     6841 gcgttaacgc cgcatccggc aggtaactcc caatcttacc gacgacgccc cagcttcact
     6901 gttttcagca ccacaaattt attattcgtg gcgatggtgg tgcagttgcc gaaaatcttc
     6961 ttcagtttat ggaagtaatc caggtgacgg ttggcaacga tatacagttc gccgttgatt
     7021 ttcaggcagc ggcgggcgtg gtggaacatc tcccaggcga cgttatcggt cagcgcatgt
     7081 tgctgatgaa acggcgggtt gcagagcaca gcattaaagc ggaaaggctc cactccggag
     7141 agcgcgttgt tgatcataaa ctcgcagcga tccaacgcct ctggcatgtt ggtttcaaca
     7201 ttcattcggc tggaagcgac cgccatcggc gattcatcga caaacaccac tttcgcctgc
     7261 gggtttttat caagcagcgt caggccaata acgccgttac cacagccgag atcgacaatc
     7321 tcaccttcga gattctctgg cagatgttgc ataaagaagc gtgcgccaat atccagcccg
     7381 gtgcgggaga agacattcgc atggttgtgg atagtccagt cagtaccttc cagcttccag
     7441 ctaacggtct gcggcgcatc ggccagcggc ggttcattga aagtgcaatt aatcaggcgc
     7501 gcttttttcc atgccagcgt agtggtggtc ggtccgagca ctttctcgaa cagctccagc
     7561 gtggaagtgt gaatgtcacg ggctttggca ccggcaataa tacgtgtatc cgacgtgacc
     7621 actttgcgca gcgcacgcag ttgctgttcc agcaatgcca gtgttttcgg cactttgatc
     7681 agcaccacac ccggctgttg cgggtagtcg gcggtgctgt cgagaaactt cacgctcgac
     7741 tcatcaatcc cgtttaggcg taaattctcg cgcgtcgcca gttcactgat gtatgagtcg
     7801 ccaatgctgt acggcttatg ttccgccagc gcacaactta acgcaccaaa ggcatcattc
     7861 aggatcaaca ccgggccgcg gatttctgtg tcgtccaact gttgcagcaa atattcatcc
     7921 gccgcttccc acgcctgtag cgggttaacg tcatccgtcg ccggaaaacg ttgcagtgtc
     7981 agtgaacgga aaccgttgtc taagtggctc atcggccctc ctgaatgata aaatttcgac
     8041 gttgcccctg aaaagggtgc gtgagtatac cctttttctc tttttcgtgg gtggttatga
     8101 gcaatcttac ttatcttcag ggttatcccg agcagctact ttcccaggtg cgaacgctga
     8161 ttaacgaaca gcgtctgggc gatgtgctgg caaaacgcta tccgggaaca cacgactacg
     8221 ccaccgataa agccctctgg caatatactc aggatctaaa aaatcagttt ctgcgcaatg
     8281 ccccgccgat caataaagtg atgtatgaca ataagatcca tgtgctaaaa aacgcgctcg
     8341 ggttacatac cgctgtttct cgtgtgcagg gcggcaagct gaaagcaaaa gcagagatcc
     8401 gcgtcgctac tgtgtttcgc aacgcgccgg aaccgttttt acgtatgatc gtggtgcacg
     8461 aactggcgca cctgaaggag aaagagcata acaaagcgtt ttaccagttg tgctgccata
     8521 tggagccgca gtaccaccag cttgagttcg acacccggct gtggctaacg cagttgtcgc
     8581 ttgggcagga caaaatctga aaatgatgtc aggcgttatc ttttctttct ttagcgccta
     8641 aaatcgacct cccccctttc gttaaggata acgaccatgc agccccgcct tcttctccgt
     8701 atttgttttt ctcgccggac tcttaagatt ggctgtttgt tgttgcttat agcaggagcg
     8761 accattttta ttgccgatcg cgttatggtc aacgccagca aacagctgac ctggagcgat
     8821 gtcaacgcag ttccggcgcg taacgtgggc ttattgctgg gggccaggcc gggtaatcgc
     8881 tactttaccc gacgtattga taccgcagca gcgttgtacc acgccggaaa agtgaaatgg
     8941 ctactggtaa gtggcgataa cggacgtaaa aattacgatg aagcctcagg tatgcagcag
     9001 gctttgatcg ctaaagacgt accggcaaaa gtgatcttct gcgactatgc cggattctca
     9061 acgctggatt cggtagtgcg tgccaaaaag gtttttggtg aaaaccatat caccattatc
     9121 tcgcaagaat ttcataatca acgcgccatc tggctggcaa aacagtacgg catcgacgct
     9181 atcggtttta acgcgccaga cctcaatatg aagcacggtt tttatactca actgcgggag
     9241 aaactcgctc gtgtcagcgc cgtgattgac gccaaaatcc tccatcgcca gccgaaatat
     9301 ctcgggcctt cggtcatgat tgggccgttt agtgagcatg gctgtccggc gcagaaataa
     9361 tgcgtatctg cgcacgtcga agatgaaaaa ggcgtgctac attgacgaca gaatcccttt
     9421 atggagtatc cacgcgttat gatacgtttc gctgtgattg gtacgaactg gatcactcgc
     9481 cagttcgtcg aggccgccca tgagagcggt aaatacaagt taaccgccgt atattcccgc
     9541 agccttgaac aggcccagca cttcgccaat gatttttctg tcgagcatct gtttacctcg
     9601 ctggaagcga tggcggaaag cgatgccatt gacgcggtgt atattgccag cccgaattcc
     9661 ctgcattttt cccagacaca acttttcctt agccataaaa ttaatgtgat ttgcgagaaa
     9721 ccgctggcgt cgaatctggc ggaagtggat gccgccattg cctgtgcgcg ggaaaatcag
     9781 gtggtgctgt ttgaggcatt taaaaccgcc tgcctgccga actttcattt gttgcgccag
     9841 gcgctgccga aagtcggcaa actgcggaaa gtctttttca actattgcca gtattcctcg
     9901 cggtatcaac gttacctgga tggtgagaat cccaacacct ttaatccggc attctctaac
     9961 ggttcaatta tggatatcgg tttttactgt ctggcgtcgg cggtggcgtt atttggtgag
    10021 ccgaaaagcg tgcaggcaac cgccagtttg ctggcaagcg gcgttgacgc ccagggcgtg
    10081 gtggtgatgg attacggtga tttcagcgtc accttgcagc actcaaaagt cagtgattct
    10141 gtcctggcga gcgagattca gggcgaagca ggatcgctgg tgattgaaaa actgtctgaa
    10201 tgccagaaag tgtgcttcgt gccgcgtggc agccaaatgc aggatctcac ccagccgcag
    10261 catattaata ccatgctcta cgaagcagag ctgttcgcta ccctggtgga tgagcatctg
    10321 gtggatcatc cagggctggc ggtcagtcgc atcaccgcca aactgctgac cgagatccgc
    10381 cgccagactg gggtgatttt tccggcagat agcgtaaaac tataattgcc aaagtaaaac
    10441 agtgtaaaag gtatgtaaca gaccattgac tggctgaatg gtctgtcata ctttgttacc
    10501 tgcaaagggg agtaacttca ttgccggtcg atcgtcatta cgatgtgtga aaaaccacat
    10561 ccggtcaccg ggcaacccga aaggaatacg cagacgtatt ccttttttgt tgtaagtgag
    10621 accttgccgg aaggcgaggt ctatgcataa aaagcagcgg ctgacgtctt ccgacgttgg
    10681 ccgttttttt atgtgtaagg aacttctatg aatactgtcg gcacgccgtt gctatggggc
    10741 ggattcgctg ttgttgtcgc cattatgctg gctatcgacc tgttgttgca ggggcgtcgt
    10801 ggggcacatg ccatgaccat gaaacaggct gcggcctggt cgctggtctg ggtgacgctg
    10861 tcgttactgt ttaacgccgc tttctggtgg tatctggtgc aaaccgaagg tcgcgccgtt
    10921 gccgatccac aggcactggc ctttctcaca ggttatctga ttgagaaatc gctggcggtc
    10981 gataacgtct ttgtctggct gatgttgttc agctatttct ctgttccgac ggcattacaa
    11041 cgccgcgtgc tggtgtatgg cgtgctcggg gcgattgttc tgcgtaccat catgatcttc
    11101 actggcagct ggctaatttc gcagttcgac tggatcctgt atatcttcgg tgccttcctg
    11161 ctgtttaccg gcgtgaagat ggcgctcgcc catgaagatg aatcaggcat tggcgacaag
    11221 ccgctggtgc gctggctacg cggtcatttg cgcatgaccg acaccatcga caacgagcat
    11281 ttctttgtgc gtaagaatgg attgctgtac gccacaccgc tgatgctggt actgattctg
    11341 gtggaattga gcgacgtgat tttcgccgtg gatagcattc cggctatctt cgccgtgacc
    11401 actgacccgt tcattgtgct gacctcaaac ctgtttgcga tcctcggcct gcgtgcgatg
    11461 tatttcctgc tggcgggcgt agcagagcgt ttctcgatgc tcaaatatgg cctggcggtg
    11521 attctggtgt ttatcggtat caagatgctg attgtcgact tctaccatat tccaatcgcc
    11581 gtctcgctgg gcgtggtgtt tggcattctg gtgatgacgt ttattatcaa cgcctgggtg
    11641 aattatcggc atgataagca gcggggagaa tgattcgctg tatattaagc cattcctaga
    11701 gataactcat tccagacaaa ggtgagctat taattatgta atcggcttgt agctctttac
    11761 aaaaagaccg cacgggtgtt ggtggcgtca aaatcgccga agcgttccct ggaggccggg
    11821 gcgacgcgca tggatgcgcg gcgagggcgc atcttacagg gacgttccct tgcgcccgac
    11881 ccggtagccg caagggataa gctgagggaa tcgcgcagcg gcgattttgt tcgccagagc
    11941 c
//
LOCUS       DAPHBJ010000137        11814 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.138, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000137 DAPHBJ010000000
VERSION     DAPHBJ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.138"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            266..1654
                     /gene="narU"
                     /locus_tag="RHZ76_003270"
     CDS             266..1654
                     /gene="narU"
                     /locus_tag="RHZ76_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HDV2813862.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1736..5476
                     /gene="narZ"
                     /locus_tag="RHZ76_003271"
     CDS             1736..5476
                     /gene="narZ"
                     /locus_tag="RHZ76_003271"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HDV2813863.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLERNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSSSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5473..7017
                     /gene="narH"
                     /locus_tag="RHZ76_003272"
     CDS             5473..7017
                     /gene="narH"
                     /locus_tag="RHZ76_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDV2813864.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7017..7712
                     /gene="narW"
                     /locus_tag="RHZ76_003273"
     CDS             7017..7712
                     /gene="narW"
                     /locus_tag="RHZ76_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDV2813865.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7709..8389
                     /gene="narI"
                     /locus_tag="RHZ76_003274"
     CDS             7709..8389
                     /gene="narI"
                     /locus_tag="RHZ76_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDV2813866.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8468..9361
                     /locus_tag="RHZ76_003275"
     CDS             8468..9361
                     /locus_tag="RHZ76_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HDV2813867.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTFWQTSLAGK
                     HRVTIEKHHDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAINKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGKVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            complement(9457..10302)
                     /gene="nhoA"
                     /locus_tag="RHZ76_003276"
     CDS             complement(9457..10302)
                     /gene="nhoA"
                     /locus_tag="RHZ76_003276"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HDV2813868.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGDYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10475..11044
                     /locus_tag="RHZ76_003277"
     CDS             10475..11044
                     /locus_tag="RHZ76_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDV2813869.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11048..11275)
                     /gene="pptA"
                     /locus_tag="RHZ76_003278"
     CDS             complement(11048..11275)
                     /gene="pptA"
                     /locus_tag="RHZ76_003278"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HDV2813870.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11326..11814)
                     /locus_tag="RHZ76_003279"
                     /pseudo
     CDS             complement(11326..11814)
                     /locus_tag="RHZ76_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
BASE COUNT         2781 a         3079 c         3077 g         2877 t
ORIGIN      
        1 gtaacccttt agattcacga tctgaagggt tgaaacatga catacgttca aagattaacc
       61 cccgtttgaa ggttagcttg ttagttacat ttagtaacac atatctggct acttaatccc
      121 cattctgccc ccgcggttac gctttcttat ctattctttc aatcagcact tcacccaatt
      181 catccttaat tctaataact ggctattgcg ccttacctcc tgttttgtct tttttcatta
      241 ccaatgtgtg catgtgagga acaatatggc actgcaaaat gagaaaaata gtcgttatct
      301 tttacgcgac tggaaaccag aaaatccggc cttctgggaa aataaaggaa aacatattgc
      361 tcgaagaaac ctctggatat cagtcagttg tctacttctt gccttctgtg tctggatgct
      421 atttagcgca gttaccgtta atctcaataa aatcggtttt aattttacta ccgatcaact
      481 ctttttatta accgcattac cctccgtttc tggcgcatta ttgcgtgttc cctactcctt
      541 tatggtgcct atattcggtg gacgccgatg gacggttttt agtactgcaa tcctgattat
      601 tccttgcgtc tggctcggaa ttgccgtgca aaatccgaat actccttttg ggatatttat
      661 cgttatcgct ttgctatgcg gttttgcagg tgcaaacttt gcttcgagca tgggcaatat
      721 cagtttcttc tttccaaaag ccaaacaagg gagcgctctt gggattaatg gcggattagg
      781 aaacttaggt gtaagtgtaa tgcagctggt tgcaccgctg gtcatttttg tacctgtatt
      841 tgcctttctc ggcgtcaatg gcgtaccgca ggccgacggt tcggtgatgt cgctggcgaa
      901 tgccgcatgg atttgggtac cgctactggc gattgccacg atcgccgcct ggtcagggat
      961 gaatgatatc gccagttcac gcgcctcaat tgccgaccag ctccctgtct tacaacgcct
     1021 gcatctctgg ctgctgagcc tgctttacct tgccaccttc ggttcgttta tcggtttttc
     1081 tgcgggtttt gccatgctgg caaaaaccca gttcccggat gtgaatattc tgcgcctggc
     1141 gttctttggc ccatttatcg gtgccatcgc gcggtcggtt ggtggtgcta tttccgataa
     1201 gttcggcggc gtgcgggtga cgttgatcaa ctttattttt atggcgattt tcagtgccct
     1261 gctgttcctt accttgccgg gcacaggctc cggtaatttc atcgcctttt acgccgtatt
     1321 tatggggctg tttctgactg cgggtctggg aagtggttct actttccaga tgatcgccgt
     1381 catctttcgc cagataacca tttatcgggt aaagatgaaa ggcggtagtg atgagcaagc
     1441 tcagaaagaa gccgtcaccg aaacggcggc ggctctgggc tttatctcag ccattggcgc
     1501 agtgggcggc ttttttattc cgcaggcgtt tggcatgtcg ctcaatatga ccggctctcc
     1561 ggtcggcgcg atgaaagtgt ttttaatctt ctacatcgtt tgtgtgctgc tgacctggct
     1621 ggtttatggt cggcggaagt tcagccaaaa ataagagatc gatgcctgat gtacaaccgt
     1681 gtcgcatccg acaaggcatc agatcccaat tcattcctgg agcaggagtc atgtcatgag
     1741 taaacttttg gatcgctttc gctacttcaa acaaaagggc gaaacctttg ccgatggtca
     1801 cggacaggtg atgcatagca accgcgactg ggaggacagc tatcgccagc gttggcagtt
     1861 cgacaaaatc gtgcgttcca cccacggtgt taactgtaca ggctcctgta gctggaaaat
     1921 ctacgttaaa aatggtctgg tgacctggga aatccaacag accgactacc cgcgcactcg
     1981 ccctgacctg cccaatcatg aacctcgcgg ctgcccgcgt ggcgcaagtt actcctggta
     2041 tctttacagc gctaaccgcc tgaaataccc gctcattcgt aaacgactga ttgaactgtg
     2101 gcgcgaagcc ctcaagcaac acagcgatcc ggtactggcg tgggcatcga ttatgaacga
     2161 tccgcaaaag tgcctgagct acaaacaagt gcgtgggcgc ggcgggttta tccgctccaa
     2221 ctggcaggaa ctaaaccagc tgattgccgc cgctaacgtc tggaccatca aaacctacgg
     2281 cccggatcgc gttgccggtt tctcgccgat cccggcgatg tcgatggttt cttacgccgc
     2341 cggaacgcgt tatctgtcgc tgcttggcgg cacctgttta agtttctacg actggtattg
     2401 cgacctgccg cccgcctcgc cgatgacctg gggcgagcaa accgacgtac cggaatctgc
     2461 cgactggtat aactccagct acatcatcgc ctgggggtct aacgtaccgc agacacgtac
     2521 gccggacgcc cacttcttta ccgaagtacg ctacaaaggc actaaaacca tcgccattac
     2581 ccctgactac tctgaagtgg ccaaattgtg cgaccagtgg ctggcaccga aacaaggcac
     2641 tgatagcgcc ctggcgatgg caatgggcca tgtgatttta aaagagtttc atctcgataa
     2701 tcccagcgac tactttatca actactgccg ccgctacagc gacatgccga tgctggtaat
     2761 gctggagcct cgcgacgatg gtagctacgt tcccgggcgc atgatccgcg catctgacct
     2821 ggtggatgga ctgggcgaaa gcaacaatcc gcagtggaaa accgtagcag ttaataccgc
     2881 aggtgaattg gtagtgccga acggttcgat tggtttccgc tggggagaaa aaggcaaatg
     2941 gaatctggaa tccattgccg ccggtacgga aaccgaattg tcgttaaccc tgctcggtca
     3001 acatgacgct gttgcaggcg tggccttccc ctactttggc ggcattgaaa atccgcattt
     3061 tcgcagcgta aaacacaatc cggtgctggt gcgccaattg cccgttaaaa acctgacgtt
     3121 agtcgatggc aacacctgtc cggtggtcag cgtttatgat ttggtactgg cgaattacgg
     3181 cctcgatcgc gggctggaag atgaaaacag tgcgaaagat tacgctgaaa tcaaaccgta
     3241 caccccagcc tggggtgagc aaattaccgg cgtgccgcgc cagtatattg aaaccatcgc
     3301 ccgtgaattt gccgataccg cccataaaac gcatgggcgc tcgatgatta tcctcggcgc
     3361 aggtgttaac cactggtatc acatggacat gaactaccgc gggatgatca atatgctgat
     3421 cttctgcggt tgtgtcgggc aaagcggtgg cggctgggca cactatgtcg gccaggaaaa
     3481 actgcgccca caaaccggct ggttgccact ggcctttgcg ctcgactgga accgaccacc
     3541 gcgccaaatg aacagcacct cgtttttcta caatcattcc agccaatggc gctatgaaaa
     3601 agtctctgct caggagttac tttcaccgct cgccgatgcc agtaagtaca gcggtcatct
     3661 gattgatttc aacgttcgcg ccgaacgtat gggctggcta ccttctgcgc cgcagctgga
     3721 gcgtaacccg ctcgggatta aagctgaagc cgacaaggcc ggattatccc ccacagaatt
     3781 taccgcccag gcgctgaaat cgggcgattt acgtatggcc tgcgaacaac cagatagcag
     3841 cagcaatcat ccgcgtaatt tgtttgtctg gcgctctaac ctgcttggct cctccggcaa
     3901 aggccacgag tatatgcaga agtatctgct ggggaccgaa agcgggattc agggcgagga
     3961 actcggtgcc agcgacggga tcaaaccgga agaagtcgag tggcaaactg cagcgattga
     4021 aggcaagctc gacctgctgg tgacgctcga cttccgcatg tccagtacct gcctgttctc
     4081 cgatatcgtt ctgcccaccg ccacctggta cgaaaaagac gatatgaaca cctcggatat
     4141 gcatccgttt attcatccgc tttctgcggc ggtcgatccg gcgtgggaat cacgcagcga
     4201 ctgggaaatc tacaaaggta tcgccaaagc attttcgcaa gtgtgcgtgg gtcatcttgg
     4261 caaagaaacc gacgtggtat tacaacccct actgcatgac tctccggcag agctctcaca
     4321 gccgtgtgaa gtgctcgact ggcgcaaagg cgaatgcgat ctgattccag gcaaaaccgc
     4381 gccgaatatt gtggcggtgg agcgcgacta ccctgctacg tatgaacgct ttacctcgct
     4441 cgggccattg atggacaaac ttggcaatgg cggtaaaggg atttcgtgga atacccagga
     4501 tgaaatcgat ttcctcggca aactcaatta caccaagcgt gatggcccgg cacaggggcg
     4561 tccgctgatt gacaccgcca ttgacgcttc agaagtgatt ctggcactgg caccggaaac
     4621 caacggtcat gttgcagtca aagcgtggca ggcgctgggc gagatcaccg ggcgcgaaca
     4681 tacccatctg gcgctgcaca aagaggacga gaagattcgc tttcgcgata ttcaggcgca
     4741 gccgcgtaaa attatctcca gccccacctg gtccggtctg gaaagcgatc acgtctccta
     4801 taacgcggga tacaccaacg ttcatgagtt aattccgtgg cgcacgctgt cgggacgcca
     4861 gcagctctat caggatcatc cgtggatgcg tgcttttggt gaaagcctgg tggcttatcg
     4921 cccgcctatc gacacccgta gcgtcagtga gatgcgccag ataccgccaa acggcttccc
     4981 ggaaaaagca cttaacttcc tgacgccgca ccagaaatgg ggcattcact caacctacag
     5041 tgaaaacctg ctaatgctga cgctctctcg cggtggaccg attgtctgga tcagcgaaac
     5101 agatgcccga gaactgacca ttgtcgataa cgactgggtg gaagtattca acgccaacgg
     5161 cgcgctgact gcccgcgcgg tggtcagcca acgtgtaccg ccgggcatga ccatgatgta
     5221 tcacgcccag gaacgcatta tgaatattcc tggttcggaa gtaactggca tgcgcggcgg
     5281 cattcataac tcggtcaccc gcgtttgccc gaaaccgacg catatgattg gcggttacgc
     5341 gcagctggcc tggggcttta actactacgg caccgtcggc tcgaaccgcg acgaattcat
     5401 catgatccgc aaaatgaaaa acgttaactg gctggatgat gaaggtcgcg atcaggtaca
     5461 ggaggcgaaa aaatgaaaat acgttcgcaa gtcggcatgg tgcttaacct cgataaatgt
     5521 atcggctgcc atacctgttc ggtgacctgt aaaaacgtct ggaccggacg cgaaggcatg
     5581 gagtacgcat ggtttaacaa cgtcgaaacc aaaccgggca ttggttatcc taaaaactgg
     5641 gaagatcagg aagagtggca aggcggctgg gtccgcgatg tgaatggcaa gatccgtccg
     5701 cgtctgggca gcaagatggg cgtaataacc aaaatcttcg ccaacccggt ggtgccgcag
     5761 attgatgatt actacgaacc tttcaccttc gactacgaac atttgcatag cgcaccggaa
     5821 ggcaaacata tccctactgc tcgcccgcgt tcactgattg acggcaaacg gatggacaaa
     5881 gtgatctggg gcccaaactg ggaagaactg ctgggcggcg agtttgaaaa acgtgcccgc
     5941 gaccgcaact tcgaggccat gcaaaaggag atgtacgggc agtttgaaaa caccttcatg
     6001 atgtacctgc cgcgcctgtg cgaacactgc ctcaatccca gttgcgtggc gacctgccca
     6061 agcggcgcta tctacaaacg cgaagaagac ggcattgtgc tgattgatca ggataaatgc
     6121 cgtggctggc gtttgtgcat tagcggttgt ccgtacaaaa aaatctactt caactggaaa
     6181 agcggcaagt cagaaaaatg tatcttctgt tatccgcgaa ttgagtcagg ccaacccacc
     6241 gtgtgctcag aaacctgcgt gggtcgcatc cggtatctgg gcgtgctgct ttacgacgcc
     6301 gaccgcattg aggaagcggc gagcaccgag cgcgaagttg acctctatga acgtcagtgc
     6361 gaagtgttcc tcgatccaca cgatccctca gtgatcgagg aagccctgaa acaaggtatt
     6421 ccacaaaacg tgattgaagc tgcccagcgt tcgcctgtct acaaaatggc gatggactgg
     6481 aaactggcgc taccgctgca ccctgaatat cgcaccctgc caatggtctg gtacgttcct
     6541 ccgctgtcgc cgattcagtc ctacgcagat gcgggcggtt tgccgaaaag cgaaggcgtg
     6601 ctgcccgcca tcgaaagcct gcgtattccg gtgcaatatc tcgccaatat gttgagtgcc
     6661 ggcgataccg gtccggtact gcgggcgctg aaacggatga tggcgatgcg ccactatatg
     6721 cgttcacaaa ccgtggaagg cgttactgat actcgtgcca tcgacgaagt aggcctgagc
     6781 gtcgcccagg tcgaagagat gtatcgttac ctcgccattg ccaactatga agatcgtttt
     6841 gtcatcccga cgagccatcg ggaaatggcg ggcgatgcct tcgcagaacg caacggctgc
     6901 ggttttacct ttggcgacgg ttgtcacggc tcggacagca aattcaacct gttcaacagt
     6961 agccgtatcg atgccatcaa catcaccgaa gtgcgcgaca aagcggaggg cgaataatgc
     7021 agatcctcaa agtgatcggc ctgttgatgg agtatccgga cgagctgttg tgggaatgca
     7081 aggaggacgc gctggcgttg atccgccgcg acgcgccgat gcttacggat ttcacccaca
     7141 acctgcttaa cgcgccgctg ctggataaac aggccgaatg gtgcgaagtg tttgaccgtg
     7201 gacgtaccac atcgctgctg ctgttcgaac atgttcatgc cgagtcccgc gatcgcggcc
     7261 aggcaatggt ggacctgctg gcggagtatg aaaaggtcgg cctgcaactg gattgtcggg
     7321 aactgcccga ttatctaccg ctgtatctgg agtatttaag cgtactgcct gacgatcagg
     7381 cgaaagaagg attgctcaat gttgcgccga tcctcgccct gcttggcggt cgcttaaaac
     7441 aacgcgaggc accgtggtac gcgttgtttg atgctctgct gcaactggcg ggaagctccc
     7501 tttcaagtga cagtgtcaca aaacaagtaa acagcgaaga gcgcgatgac acccgccagg
     7561 cgcttgatgc agtgtgggaa gaggaacagg tgaagtttat tgaagataac gccacggcgt
     7621 gtgacagctc gccgcttaat caatatcagc gacgctttag ccaggatgtc gcgccgcaat
     7681 atgtcgacat cagtgcggga ggtgggaaat gattcagtat ctgaacgtct ttttttacga
     7741 tatctacccg tacatttgtg cgacggtgtt tttcctcggc agctggctgc gctacgacta
     7801 cgggcagtac acctggcgcg cctcctcaag tcaaatgctc gataaacgcg ggatggtgat
     7861 atggtcgaat ctgttccata tcggcatttt ggggattttc ttcgggcact tgttcggcat
     7921 gttaacgccg cactggatgt acgcgtggtt tttgccagtg gcagcgaaac aactcatggc
     7981 gatggtgctc ggcggtattt gcggtgtgtt gacgttgatt ggcggcgcag gactgctgtg
     8041 gcgcaggctg acaaatcagc gcgtgcgtgc cacttccact acgccggata tcatcatcat
     8101 gagcattttg ctgatccagt gtctgttggg gctaagtacc ataccgtttt cagcacagta
     8161 tcctgatggt agcgaaatga tgaagctggt gggttgggcg caaagcattg tcactttccg
     8221 tggtggatcg tcagagatgc tcaacggcgt agcgttcgtc ttccgcctgc atctggtgtt
     8281 gggaatgacc atcttcctgc tcttcccgtt cacccgactg gtgcatgtgt ggagcgcgcc
     8341 gtttgagtac tttactcgtc gatatcaaat tgtgcgttcg cggcgataat ttcaccctgg
     8401 ccggatagcc cttcgctctc cggccacttg caggctatga tagttctcca gtcacaggag
     8461 aacgttaatg aaaccgcagg tgtaccacgt cgatgctttt acctcacaac cgtttcgcgg
     8521 caattctgcc ggtgttgttt tccccgccga taatcttagc gaagcgcaaa tgcagcttat
     8581 cgcccgcgag ttaggccatt cggaaactgc ttttctgctg cacagcgacg acagcgatgt
     8641 gcgcatccgc tactttacgc caacggttga agttccgatt tgtggtcacg cgacagtagc
     8701 tgcgcactat gtacgtgcga aggtgttagg tttaggaaat tgcacgttct ggcaaacatc
     8761 gctggcagga aaacatcgcg tgactatcga aaagcaccac gatgattatc gtatttcgct
     8821 ggaacaaggt acgccgggct ttgagccacc gctggaaggt gaaacacgtg aggcgattat
     8881 caacgcgctt catctcactg aggacgatat tctgccaggc ttgccaattc aggtggcaac
     8941 aacaggacac tcaaaagtga tgatcccgtt gaaaccggaa gtggatatcg acgccctttc
     9001 tcctgacctt aatgcgctga ccgctatcaa caaacagatt ggctgcaatg gtttcttccc
     9061 gttccagatc cgcccaggca aaaacgaaac cgatggtcgc atgttctcgc ctgcgattgg
     9121 tattgtggaa gatccggtga ctggcaatgc caatggcccg atgggtgcat ggttggtaca
     9181 tcacaacgta ttgccccacg atggcaaggt gctgcgtgtt aaaggccatc aggggcgcgc
     9241 attggggcgt gacggcgtga ttgaagtgac ggtgacaatt cgtgataacc aaccggaaaa
     9301 agtcaccatt tctggcgcgg cggtgatttt gtttcatgcc gaatgggcaa ttaaactctg
     9361 aaaccgttgc ctgatgcgct tcgcttatca ggtctacaac cgagctaatg tatgccggat
     9421 aaggcgttta cgccgcatcc ggcaacctga cataaattat tttcccgcct ccgggtgcgt
     9481 atcaaaagcc gccatcacca gcgctaactc atccacggta aagccatgtt tcgcatcatc
     9541 cacgcccaga ccaaactgtt cttgcatcac agcatataat gacgccacat ccggtagatt
     9601 tcgctgctcc accgcgtgcc cattttcata atgggtaaaa tgaaaattgg tcagtgtcag
     9661 cttgccgccg tccggcaaat ggcggcacat cagcaaatga tggcgaaaat gcgactgcgg
     9721 ccagtgcgcc gaccagaaat tgcccatcac ataatcgctt tgttgctgct cgcagagatc
     9781 aaaacggtac atcgactgcc agtgctgatg atgattaaac tgcaacaccc aatcatcacc
     9841 ctcctgcaac aaccgataat ctccgtgtgg cgtggtctgc acgagatcgg aaactaaacg
     9901 aatcggcgcg gttagcgtct gcccaccgaa accgacatca gcaatccatt tttcctcttc
     9961 cagttccacc aacagcaaac gatgggtgcg cggcggtaat gctggcggat ttgataacac
    10021 tacgcgccct aacaagctgc gaacgttaaa ctccagctcg cgtaacaccc gctcaaacac
    10081 gccattctgc tcaaaacagt aaccgccacg acgggctatc accagtttct cttccagcga
    10141 ttgatcatca agctgtattt ccctcggcag caaaacgtcg aggttttcaa acggaatagt
    10201 gcaattgtgt ttcaggtgca atgcacgaag cgtatcaata ttgaccgcag cagctcccga
    10261 ccagttaata cgggcaaaat agtgattcag aatgggcgtc atcaggcgtt tcccttagtg
    10321 gttttctcaa cttataacct ggaatagacg ccgcaccggc tattttgtgc gatcgcggac
    10381 atgcatcacg tttgcaaatc gccaatcccg ttaatttcgt tacttattgc gttacggact
    10441 acgcttaaaa aaaccttctt cgcgaggcga taccatgagc cgattcatcc ccatagaatt
    10501 acaccatgcc agccgtctgt taaatcatgg cccaactgtc atgatcacca gtttcgatga
    10561 gcaatcccag cggcgtaaca ttatggcagc agcctggtca atgccggtgg agtttgaacc
    10621 gccacgcgtg gcgattgtgg tagataaatc gacatggacc agggagttga ttgaacgtaa
    10681 cggtaaattt ggcatcgtta tcccgggcgt tgcagcaact aactggacgt gggcagtggg
    10741 aagcgtgtcg ggtcgtgaag aagataaatt taattgctat ggcattccgg tggtgaaagg
    10801 tccggtgctt ggtttgccac ttgttgaaga gaaatgcctg gcgtggatgg agtgccgcct
    10861 gctacctgcg acttctgcgc aacaaaaata cgacacgctg tttggcgagg tggtatcagc
    10921 agcggcagac gcacgggtat ttgtcgaagg ccgctggcag tttgatgatg ataagctcaa
    10981 tacgttgcat catttaggtg ctggcacgtt tgttaccagc ggcaagcgag ttacggcggg
    11041 ttaagcatta catgctgtaa ccaggtttct ttatcaaagc ctccatttgg ggcgcgattt
    11101 cggcatccca gatagcttgc caagattctg gttgaatctg ctgtagagca atgcttatcg
    11161 aactgtcttt actgttcaga tggcgaataa taacgtcggt aatatctgca gcaagtgctg
    11221 ctttttgttg ttcgtccagt tcacgcggaa aacatttaat gtcgatgtgc ggcatgattg
    11281 tgctcctttt gaaagatgtc agtaaaatta acacacattg tgaattttca gtcctttcca
    11341 ttcaaacgtg aaatcaataa gttcatcagg gacatcgcaa acaatatgaa gacggatttc
    11401 ttctctgccg tgactctttt cactgattgc gtaactgtca tgctctggat tatttaattc
    11461 tttcagcgga aatttttcct caaaggcttt atttagccgc ccctggtttc cttttaccgc
    11521 gaataaataa tcacctccct gtttttgtat cttctctgca atatctttct ggcaacccat
    11581 cgcatcagtt gtgatgattt ttcctttaat atccagcatg ttaagaagtt ctgggatagc
    11641 tgtaatctca ttagatttct catccgtctt gatctgtccg atgaccagac tgtgcattgt
    11701 tgagaacgca ctaatgacat gaatcgctcc cctgcggcga ctcttatcat aagaatgccg
    11761 gagcgttttt ccatcaattg caatgacgtc tttatcatct gaagaatgac aatc
//
LOCUS       DAPHBJ010000138        11765 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.139, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000138 DAPHBJ010000000
VERSION     DAPHBJ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.139"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(37..318)
                     /locus_tag="RHZ76_003280"
     CDS             complement(37..318)
                     /locus_tag="RHZ76_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HDV2813871.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(581..1690)
                     /gene="apbC"
                     /locus_tag="RHZ76_003281"
     CDS             complement(581..1690)
                     /gene="apbC"
                     /locus_tag="RHZ76_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDV2813872.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1822..3855
                     /gene="metG"
                     /locus_tag="RHZ76_003282"
     CDS             1822..3855
                     /gene="metG"
                     /locus_tag="RHZ76_003282"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDV2813873.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLANELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3996..7790
                     /locus_tag="RHZ76_003283"
     CDS             3996..7790
                     /locus_tag="RHZ76_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HDV2813874.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAKLKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIEWDYANNVIIILPSGIAPSYLEQYSRFELR
                     LRKHLSLTEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGQTKRCHDRMTKAIAAFPHAAMAALTELLGQK
                     EENSWRIMLMTMLISQPALAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAVV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPICYLTEEISNQLLAKYIWYSKHITVSHEESTT
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYSMLHFGLKGLPWLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRENARSWLLKYPEHALTGLLPAALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDSALLHLGEMLRFPQEEALYPGLLQVKDVCSADSLAGFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDVSGSRLKDLPKPNKSDDETRANDAVNRYKLLKKDARTIAAQQVAR
                     LESAMCLRRRWSLENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSSST
                     ADDDLFTLPEGDISIGTPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWIKGEPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPAELSAEQLLSKLWLWEGKAERYGWGSNSTQEAQFSVIDAITACELINDIEAL
                     FE"
     gene            7800..11432
                     /locus_tag="RHZ76_003284"
     CDS             7800..11432
                     /locus_tag="RHZ76_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HDV2813875.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIARCYESDPLVVTLQYQDEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELVGL
                     ECPRTHFHSKEWLKVVANDPTAVRKLEHYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAITRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYHKNFP
                     HATLAALAELLALTEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAE
                     VMPWLSTQPQSVLKSYLSAPPKPVIDGTDNSNLPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGEQERLAATEPARYFSTESLAQRMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLFVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSHYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVAHVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALHLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     GYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMAVEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDKSTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDTF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSCRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11493..>11765
                     /locus_tag="RHZ76_003285"
     CDS             11493..>11765
                     /locus_tag="RHZ76_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813876.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCPELFTGSVYRLER
                     FRLHPTFHQRDREDADPLINDALIYIRDECNDERKLRGESPETVIAI"
BASE COUNT         3053 a         3019 c         2907 g         2786 t
ORIGIN      
        1 cggctttcta aatgcaatta aaatcaggtt gccaaattaa tcgtagcgat ttaacacgat
       61 tcgacgtttg ctatcgctac ctgcaacaac cacttctgtc gttacacctt tgacgggcga
      121 agaaaacaga tgacgggtgt caacattata cgttaactga ccacaatgct gttgaggttg
      181 accattaacc atagccatac tgcatgttgg gggactgatc ctgccattaa tcgctaatgt
      241 ggcagtctct gaagagaatg cgggagaagc caatccatag gcaagaaaaa taattcctga
      301 cagccaatac ttattcattg aacgttatcc ctgtagtaaa ggttatgcct ggatagaatg
      361 agtgcataac aaactatagc tgtacatcca ctacacagcc acgaaggatg ataatgaagc
      421 attgcctgta tgatcaatcg actttgtaga gtttcggacg aaggtccgca gaatattcgc
      481 agtattaaat aagtgttcat taatgcggca aaaactaata caccgcatca atgtaacatc
      541 tctgcggtgt atgaagaata accagaatgg ttaataggcg ttagaccgcg cggaaggaaa
      601 tctcgcctgg aatgacttca ccctgccagt agagctgagc tgcaacgcgg tcagccagct
      661 ggcggtagat agcggtaaat tcgctctccg gacggctgat aaccgttggc gtacctttat
      721 cgagatcctc acgtaaggag atgtgtaacg gcatctgccc cagcagctgt gtatggtatt
      781 tttcagccag tttctctgca ccaccggtac cgaaaatcgg ttcatggtga ccacagttac
      841 tgcaaatatg cacactcatg ttctcgacaa taccgagcac cggaacctcg actttctcga
      901 acatcacaat gccttttttc gcatcgatca gcgcgatgtc ctgcggggtg gtgaccacga
      961 ctgcaccggt tactggaatg ttttgcgcca gcgtcaattg aatgtcaccg gtgcctggcg
     1021 gcatatcaag caccagatag tcgagatccg gccacaaggt ttcctgcaac atctgcatta
     1081 acgccttgct cgccatcggt ccacgccaca ccatcgcatt gtcgtcagtg accagataac
     1141 caattgaatt ggttgccagg ccatgagaca taatcggtgc catatgggta ccgtccggcg
     1201 aagttggacg ctgattttct gcgcccaaca ttgttgggat tgatgggccg tagatatcgg
     1261 cgtccagaat gccgactttc gccccttctg ccgccagcgc cagtgccagg tttaccgccg
     1321 tggacgattt acccacgccg cctttaccgg aactcaccgc gataatgttc ttcacaccgt
     1381 taattcccgg ctgattcttc acgcgtttca gagtggctat gttatgcgac agtttccagt
     1441 cgatagcttt cgccccggtg atgcgcagca gttcggcgct acattgctct tttaactctt
     1501 cgaacgcgct atgccagaca aacggcataa ccagttccac atgcaacgtg tcgtccatcc
     1561 aggcgacatg gtgcaacgct ttcagcgtgg tgaggttatg cttcagggtt gggtgctgaa
     1621 aattggccag tgtcccggcg accattgcgc gcaaggcttc tggtgatttg gcctgggatt
     1681 gttcgttcat cccgactcct tttttgtata gataaaccat cagctgatag tttacctgaa
     1741 gaatatagag aagtacttac ttaacatttt cccatttggt actatctaac cccttttcac
     1801 tattaagaag taatgcctac tatgactcaa gtcgcgaaga aaattctggt gacgtgcgca
     1861 ctgccgtacg ctaacggctc aatccacctc ggccatatgc tggagcacat ccaggctgat
     1921 gtctgggtcc gttaccagcg aatgcgcggc cacgaggtca acttcatctg cgccgacgat
     1981 gcccacggta caccgatcat gctgaaagct cagcagcttg gtatcacccc ggagcagatg
     2041 attggcgaaa tgagtcagga gcatcagact gatttcgcag gctttaacat cagctatgac
     2101 aactatcact cgacgcacag cgaagagaac cgccagttgt cagaacttat ctactctcgc
     2161 ctgaaagaga acggttttat taaaaaccgc accatctctc agctgtacga tccggaaaaa
     2221 ggcatgttcc tgccggaccg ttttgtgaaa ggcacctgcc cgaaatgtaa atccccggat
     2281 caatacggcg ataactgcga agtctgcggc gcgacctaca gcccgactga attgatcgag
     2341 ccgaaatcgg tggtttctgg cgctacgccg gtaatgcgtg attctgaaca cttcttcttt
     2401 gatctgccct ctttcagcga aatgttgcag gcatggaccc gcagcggtgc gttgcaggag
     2461 caggtggcaa ataaaatgca ggagtggttt gaatccggcc tgcaacagtg ggatatctcc
     2521 cgcgacgcgc catactttgg ttttgaaatt ccgaacgcgc cgggcaaata tttctacgtc
     2581 tggctggacg caccgattgg ctacatgggt tctttcaaga atctgtgcga caagcgcggc
     2641 gacagcgtaa gcttcgatga atactggaag aaagactcca ccgccgagct gtaccacttc
     2701 atcggtaaag atatcgttta cttccacagc ctgttctggc ctgccatgct ggaaggcagc
     2761 aacttccgca agccgaccaa cctgtttgtt catggctatg tgacggtgaa cggcgcgaag
     2821 atgtccaagt ctcgcggcac ctttattaaa gccagcacct ggctgaatca ttttgacgct
     2881 gacagcctgc gttactacta cactgcgaaa ctctcttcgc gcattgatga tatcgatctc
     2941 aacctggagg atttcgttca gcgcgtgaat gccgatatcg ttaacaaagt ggttaacctg
     3001 gcctcccgta acgcgggctt tatcaacaaa cgttttgacg gcgtgctggc aaacgaactg
     3061 gctgacccgc agttgtacaa aaccttcact gatgccgctg aagtgattgg tgaagcgtgg
     3121 gaaagccgtg aatttggtaa agctgtgcgc gaaatcatgg cgctggctga tctggctaac
     3181 cgctatgtcg atgaacaggc tccgtgggta gtcgcaaaac aggaaggtcg cgatgctgac
     3241 ctgcaggcga tttgctcaat gggcatcaac ctgttccgcg tgctgatgac ttacctgaag
     3301 ccggtactgc cgaaactgac cgagcgtgca gaagccttcc tcaatacgga actgacctgg
     3361 gatggtatcc agcaaccgct gctgggccac aaagtgaatc cgttcaaggc gctgtataac
     3421 cgcatcgata tgaaacaggt tgaagctctg gtggaagcct ctaaagaaga agtgaaagca
     3481 gctgccgcgc cagttactgg cccactggca gatgatccga ttcaggaaac catcaccttt
     3541 gacgacttcg ccaaagttga cctgcgcgta gcgctgatcg aaaacgcaga gtttgttgaa
     3601 ggttctgaca aactgctgcg cctgacactg gatctcggcg gtgaaaaacg caatgtcttc
     3661 tccggtattc gttctgccta cccggatccg caggcactga ttggtcgtca caccattatg
     3721 gtggctaacc tggcaccacg taaaatgcgc tttggaatct ccgaaggcat ggtgatggct
     3781 gccggtccag gcgggaaaga tatcttcctg ctaagcccgg atgctggtgc taaaccgggt
     3841 catcaggtga aataatcccc cttcaaggcg ctgcatcgac agcgcctttt ctttataaat
     3901 tcctaaagtt gttttcttgc gattttgtct ctctctaacc cgcataaata ctggtagcat
     3961 ctgcattcaa ctggataaaa ttacagggat gcagaatgag acactttatc tatcaggacg
     4021 aaaaatcaca taaattctgg gcggtggagc aacagggaaa cgagttgcat atcagttggg
     4081 gaaaagttgg caccaaaggg caaagtcaga taaaaagttt ttcagatgct gcggcagcgg
     4141 aaaaagcgaa acttaagctg attgcggaga aggtgaagaa ggggtatgtg gagcaagcga
     4201 aggataattc tttgcaacct tcccaaacgg taacgggctc tctcaaggta gcggacttat
     4261 ccaccattat tcaggaacaa ccctctttcg tagcagaaac ccgtgcgcct gacaaaaata
     4321 cagatgctgt tttaccgtgg ctggcgaaag atattgctgt cgtttttccg cccgaagttg
     4381 tacacaccac gttaagtcat cgccgctttc ccggagttcc tgttcagcaa gcagacaaat
     4441 tgacccaatt acgtcgctta gcctgtagtg tgtcgcaacg ggataataaa acagccacat
     4501 ttgacttcag cgcctgttct ttagaatggc aaaacaccgt cgcccaggcg atcagtcaga
     4561 tcgacggcct gaaaacaaca cagttaccat caccagtaat ggctgtactc acggcacttg
     4621 aaatgaaatg cacaagatat aaagtgcgtg aggatgttat ggatcagatc gtccaggaag
     4681 gcggtctgga atatgctact gatgtaataa tacaccttca acagattgat attgaatggg
     4741 attatgcgaa taatgtcatt attattctgc cgtctggcat tgcacctagc tacttggagc
     4801 aatattccag atttgaatta cgcctacgta aacatttatc actgacggaa gagtctctct
     4861 ggcaaaaatg tgcacaaaaa cttattgccg caattccaca tattccagaa tggcggcaac
     4921 cattaattgc tttgttatta cccgaaaaac cagaaattgc acatgaaatt gcccagcgtc
     4981 tactggggca aaaaaaatta ccctcgcttg agtggttaaa aatagtggcg actgatgagc
     5041 acattcttgc ctcattagaa aaatatcacg aaccatatgc catttttgat gattactatt
     5101 gtggtgcgat atggtcagcc accgtattac aggagcaagg tgttgcagcc ctgccccgat
     5161 ttgctcccta tgccgcaagt gactactgcg ccgatgtgtt gcgtcatatc aatcatccgt
     5221 tcgcattgac actgcttata cgtgtagccg ggcaaactaa acgctgtcac gatcggatga
     5281 cgaaagccat tgctgcgttc ccacatgcag caatggcggc actgacggaa cttcttgggc
     5341 aaaaagaaga gaacagttgg cgcattatgc taatgacaat gcttatctca caaccagcac
     5401 tggcagaaca ggtcattccc tggctctcga cacccgcagt tgccgtactg aaatcatgcc
     5461 agcaacaact gacacagccc tcaaaccatg ccagcgccga tctactgcca gccgtagtag
     5521 tctcccctcc ctggctttcg aaaaagaaaa aatcgccgat tccggtgctg gatttagcgc
     5581 cattaggcat tgagccaatc tgttatctga cagaagaaat cagtaatcaa cttttggcga
     5641 aatatatctg gtattcaaaa cacatcacgg ttagccatga agaaagtact accaacctgt
     5701 tggcaaggat gggttttcaa cgacggatcg ctggtacata tattaaagct cccgaagcgg
     5761 tagttgaggc atggctaaat gaagattatt caaccttact aagtgaattt aaggtgtttc
     5821 attcacctac cgggcattat tggcagttgg ggattttgac aacattgccg ctggagaaag
     5881 cagtaaaagc atggaatgcc cttaccctat ctccacatac cgataccgaa tactccatgt
     5941 tacattttgg actcaaaggg ttaccttggt tagtaaactc acttgcacgc tatccacaag
     6001 aagccttgcc catcacgaat tacttcgcag cgagtgagct ggctcctgcc gtcgcccgtg
     6061 ccttcaacaa actgaaaacg ctacgcgaaa acgcccgtag ctggctgttg aaatacccag
     6121 aacatgccct taccggcctg ctgcctgcgg cgctcggcaa agccggtgaa gcacaggata
     6181 acgcccgcgc tgccttgcgt atgcttaccg aaaacggtca tcagccatta ctgcaagaaa
     6241 tcgcccgacg ttataaccag ccggaagtaa ccgatgcggt gaacgctctg cttgcgctcg
     6301 atcccttaga taatcacccg acaaaaatcc ccactcttcc ggccttttat cagccatcgc
     6361 tctggacgcg cccggtatta aaagcaaatg cccaatcact gccagatagc gccctcctcc
     6421 acctcggtga aatgctccgc ttccctcagg aagaggctct gtatccggga ttattgcagg
     6481 tgaaagacgt ctgttccgcc gactcactgg cgggatttgc ctgggatctg tttaccgcct
     6541 ggcagaccgc tggcgcgccg tcgaaagaga gttgggcgtt cactgcgtta ggcgttctcg
     6601 gtaacgatga caccgcccgc aaactgacgc cattaatacg cgcctggcct ggtgaatccc
     6661 agcataaacg cgccaccgtt gggttggata ttctcgctgc tatcggtagt gatatcgccc
     6721 ttatgcagct taacggcatc gcccagaaac tgaaattcaa agcattacag gagcgggcaa
     6781 aagaaaaaat tgccgacatt gccgagagcc gcgaactcac ggtggcggag cttgaagatc
     6841 ggttagcacc ggatctcggt ctggatgata acggttcgct gctgctggat tttggcccac
     6901 ggcagttcac cgtcagcttt gatgaaacct taaaaccgtt tgtgcgtgat gtttccggca
     6961 gccgcctgaa agacctgccc aaaccgaaca aaagcgatga tgaaacgcgg gcgaacgatg
     7021 cggttaaccg ctacaaattg ctgaaaaaag atgcgcgtac catcgccgcc cagcaggtag
     7081 caaggctgga atccgccatg tgcctgcgcc gccgctggtc gctggaaaac ttccagctct
     7141 tcctggttga gcatccgctg gttcgtcact taacccgccg tctgatttgg ggcgtttata
     7201 gcgccgaaaa ccagctactg gcttgctttc gcgtagcaga agataacagc tccagcaccg
     7261 ctgacgatga tcttttcacc ctgccggaag gcgatatctc tatcggcact cctcacgttc
     7321 tggaaatatc accaacggat gctgccgcct ttggtcagct ttttgccgac tacgaactgc
     7381 taccaccgtt ccgccagctc gaccgtaaca gctacgccct gacagaagcc gagcgcaatg
     7441 ccagtgaact gacccgctgg gcaggcagaa aatgcccgag tggtcgggtc atggggctgg
     7501 cgaataaagg ctggataaag ggcgaaccac aggatggagg ctggatcgga tggatgatca
     7561 aacctttggg tcgctggtcg ttaatcatgg aaatcgatga aggctttgcg gtaggcatgt
     7621 cgccagccga actcagcgct gagcagctct taagcaagct gtggctatgg gaaggcaaag
     7681 cagaaagata tggctggggg agtaattcaa cacaggaagc gcagttctcc gtaatcgatg
     7741 ccatcaccgc ctgcgagcta attaacgata ttgaagccct gtttgaataa ggaaagagca
     7801 tggacaagga attaccgtgg ctggcggata acgcccaact ggaactgaaa tataaaaaag
     7861 gcaaaacgcc gctcagtcat cgtcgctggc cgggcgaacc agtgtccgtt atcactggaa
     7921 gtctcatcca gacattgggt gatgaattgc tacaaaaagc tgagaagaaa aaaaacattg
     7981 tctggcgtta tgagaatttt tcactggagt ggcagtccgc catcacgcag gccatcaact
     8041 tgatcggcga acacaaaccc tcaatcccgg cccggacaat ggcggcgcta gcctgtatcg
     8101 cgcaaaatga cagccaacag ttgctcgacg aaatcgtcca acaagagggg ctggaatatg
     8161 cgactgaggt ggtgattgca cgccagttta ttgcgcggtg ttatgagagt gatcctctgg
     8221 tagtgacatt gcagtatcag gacgaggatt atggctatgg ttatcgctca gaaacctata
     8281 acgaattcga tctccgactg cgtaagcatc tctctctggc agaggaaagc tgctggcagc
     8341 gttgcgccga caaactcatt gccgcactac caggaataac caaagttcgc cgccctttta
     8401 ttgcgctgat cctcccggaa aaaccagaaa tagccaatga gttggtaggc cttgaatgcc
     8461 cgcgaactca ttttcattct aaggagtggt taaaagttgt tgctaatgac cccacagcgg
     8521 tgagaaaact cgaacactac tggagccagg atatatttag cgatcgagaa gccagctaca
     8581 tgtcgcatga aaaccacttc ggctacgcgg cctgcgccgc ccttttgcgc gaacaaggac
     8641 tggcagccat tacgcgcctc gcgatgtatg cccataaaga agattgcggc agtctgctgg
     8701 tacaaattaa ccatccgcaa gtcatccgca ccttgctact ggtggctgat aaaaacaaac
     8761 ccagcctgca acgtgtagct aaataccata aaaacttccc ccatgcgacg ctcgccgcac
     8821 tggcagaact gctggcgtta acagaaccac cagcccgccc tggttatcca atcatcgaag
     8881 acaaaaagct gcctgcacag caaaaagcac gcgatgaata ctggcgtacg ctgttacaaa
     8941 cgctgatggc atcgcagcca caactggcag cagaagtgat gccgtggtta agtactcaac
     9001 cccagtcagt gctgaagagt tatttatcgg caccgcccaa accggttatt gatggcaccg
     9061 ataacagcaa tctgccagaa atcctcgttt caccaccgtg gcgtagtaag aaaaaaatga
     9121 cagctccacg tcttgatttg gcaccgctcg aattaactcc gcaagtttac tggcaaccag
     9181 gcgaacaaga gaggcttgcc gccactgagc ctgcccgtta tttcagcacg gaatctcttg
     9241 cgcaacgcat ggaacaaaaa agtggacgag ttgtattaca ggaactgggt tttggggatg
     9301 atgtatggct gtttctgaat tatatactcc ccggaaaact ggatgctgca cgcaattcac
     9361 tctttgttca gtggcattac taccaggggc gggttgaaga gatcctgaat ggctggaact
     9421 ccccggaagc acaattagca gaacaggcgc tccgcagcgg tcacatagaa gcgttaatta
     9481 acatatggga aaatgacaac tactcacatt atcgtccgga aaagagtgtc tggaacctgt
     9541 atttattggc acagttgccg cgtgagatgg ctttgacctt ctggctgcgt atcaatgaga
     9601 aaaagcatct gttcgcgggt gaggactatt ttctcagtat cctcggattg gatgcgctac
     9661 caggtctgct gttggctttt tcacatcgtc caaaagaaac atttccgtta attttaaatt
     9721 ttggcgcaac agaactggcg ctgcctgttg cccacgtctg gcgacgtttc gcggcgcagc
     9781 gtgatctggc tcgccagtgg attttacaat ggccggaaca tacggctagt gcacttatcc
     9841 ctcttgtctt taccaaaccc agcgataata gcgaagccgc attacttgcc ctgcatttac
     9901 tgtacgaaca gggacatggc gaattgctac aaaccgtggc aaaccgctgg cagcgtacag
     9961 atgtatggtc tgccctggag cagttgctta aacagggtcc aatggacatt tacccggcac
    10021 gcattccaaa agcccctgat ttctggcatc cggcaatgtg gtccaggcct cgacttatca
    10081 ctaataatca gcctgttacc ggtgacgctc tggaaattat cggcgaaatg ctgcgcttta
    10141 cccagggggg acgtttttat agcgggctgg aacaactgaa aacgttctgc cagccacaaa
    10201 cgctggcagc ttttgcctgg gatctcttca ctgcgtggca acaagctggt gcccccgcaa
    10261 aagacaactg ggcatttctg gcgttaagtc tctttggtga cgaaagcacg gcacgggatc
    10321 tgacgacaca gatcctcgcc tggccacaag aaggcaaatc tgcccgtgct gtcagcggcc
    10381 tgaacatcct taccctgatg aataatgata tggcgctgat acagctgcat catatatcgc
    10441 aacgggctaa atcccgcccc ttacgtgata acgcggcgga atttcttcag gtggtcgcag
    10501 aaaatcgcgg gctaagccag gaagagctag cggacagatt agtcccaacc ctgggccttg
    10561 atgatccgca ggcgttgagt tttgattttg gtccccggca gtttaccgtt cgcttcgatg
    10621 aaaacctcaa cccggttatc tttgatcagc aaaacgttcg ccagaaaagc gttccccgtt
    10681 tgcgcgccga tgacgatcaa ctgaaagcgc ccgaggcact ggcccgacta aaagggctaa
    10741 aaaaagatgc tactcaggtg agcaaaaacc tgctcccgcg tcttgaagct gccctacgta
    10801 ccacccgacg ctggtcgctg gcagattttc attctctgtt tgttaatcat ccctttaccc
    10861 gtctggttac ccagcgatta atatgggggg gttatccggc aaatgaaccg cgtcgtttac
    10921 tcaacgcctt tcgtgtggcc gcagaggggg agttctgcaa tgcgcaagat gagccaattg
    10981 acctgcctgc ggacgctctg attggcattg cccacccgtt agaaatggca gtagaaatgc
    11041 gcagtgaatt tgcacagctt tttgccgatt acgaaattat gccgcctttt cgccagttgt
    11101 cgcgccgcac ggtgctgctc acacctgaca agtcaaccag taacagcctg actcgctggg
    11161 aaggtaaatc cgctaccgtt gggcaactta tgggaatgcg atacaaaggc tgggagtcag
    11221 gctatgagga cacatttgtc tatgacctgg gcgagtaccg gctggtcctt aagttttcac
    11281 ccggttttaa ccactacaat gttgatagca aagcgttaat gagctgccgt tctcttcgag
    11341 tgtaccgtga caataaatcc gtcacttttg ccgaacttga tgtgtttgat ttgagtgagg
    11401 cgttaagcgc acctgacgtc attttccatt aaaacacagc gggcagtgat gcgactgccc
    11461 gttatctaca cgacttacca gcggggaaag cgatgatcgt gcaaaaagag ctggttgcta
    11521 tttacgatta tgaggtccct gtacctgaag atccgttttc cttcagactt gagatccata
    11581 aatgccctga attatttaca ggttccgtct atcgactgga gcgattccgg ctacatccaa
    11641 catttcatca acgtgatcga gaagatgctg acccgctaat aaatgatgcg ttgatttata
    11701 taagagatga gtgtaatgat gagcggaaat tacgaggtga atcacctgaa actgtaatag
    11761 caatt
//
LOCUS       DAPHBJ010000139        11282 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.140, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000139 DAPHBJ010000000
VERSION     DAPHBJ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.140"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(40..630)
                     /gene="paaY"
                     /locus_tag="RHZ76_003286"
     CDS             complement(40..630)
                     /gene="paaY"
                     /locus_tag="RHZ76_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HDV2813877.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVGEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPGRKRLVFDENLRPKQ"
     gene            complement(612..1562)
                     /gene="paaX"
                     /locus_tag="RHZ76_003287"
     CDS             complement(612..1562)
                     /gene="paaX"
                     /locus_tag="RHZ76_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HDV2813878.1"
                     /translation="MSKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKATLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKETAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEALCQFTR"
     gene            complement(1663..2976)
                     /gene="paaF"
                     /locus_tag="RHZ76_003288"
     CDS             complement(1663..2976)
                     /gene="paaF"
                     /locus_tag="RHZ76_003288"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HDV2813879.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     NAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(3003..4208)
                     /gene="paaJ"
                     /locus_tag="RHZ76_003289"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(3003..4208)
                     /gene="paaJ"
                     /locus_tag="RHZ76_003289"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HDV2813880.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRSAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(4208..4630)
                     /gene="paaI"
                     /locus_tag="RHZ76_003290"
     CDS             complement(4208..4630)
                     /gene="paaI"
                     /locus_tag="RHZ76_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HDV2813881.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(4620..6047)
                     /gene="paaC"
                     /locus_tag="RHZ76_003291"
     CDS             complement(4620..6047)
                     /gene="paaC"
                     /locus_tag="RHZ76_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaC"
                     /protein_id="HDV2813882.1"
                     /translation="MMINLQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAADLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEIKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVIDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(6049..6837)
                     /gene="paaB"
                     /locus_tag="RHZ76_003292"
     CDS             complement(6049..6837)
                     /gene="paaB"
                     /locus_tag="RHZ76_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase"
                     /protein_id="HDV2813883.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAESLKQAER
                     DDTIRCLLITGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFMGK"
     gene            complement(6837..7604)
                     /locus_tag="RHZ76_003293"
     CDS             complement(6837..7604)
                     /locus_tag="RHZ76_003293"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase"
                     /protein_id="HDV2813884.1"
                     /translation="MSELIVSRQQRVLLLTLNRPAARNALNNALLTQLVNELEAAAID
                     TSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITARQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRSPDFKGR"
     gene            complement(7601..8671)
                     /gene="paaK"
                     /locus_tag="RHZ76_003294"
     CDS             complement(7601..8671)
                     /gene="paaK"
                     /locus_tag="RHZ76_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HDV2813885.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASFDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDDAETALKALGMPEKAIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(8679..9176)
                     /gene="paaD"
                     /locus_tag="RHZ76_003295"
     CDS             complement(8679..9176)
                     /gene="paaD"
                     /locus_tag="RHZ76_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate degradation protein PaaD"
                     /protein_id="HDV2813886.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(9191..9937)
                     /gene="paaC"
                     /locus_tag="RHZ76_003296"
     CDS             complement(9191..9937)
                     /gene="paaC"
                     /locus_tag="RHZ76_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HDV2813887.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESREPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAIN
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(9946..10233)
                     /gene="paaB"
                     /locus_tag="RHZ76_003297"
     CDS             complement(9946..10233)
                     /gene="paaB"
                     /locus_tag="RHZ76_003297"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HDV2813888.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYSIPDGIEHM"
     gene            complement(10245..11174)
                     /gene="paaA"
                     /locus_tag="RHZ76_003298"
     CDS             complement(10245..11174)
                     /gene="paaA"
                     /locus_tag="RHZ76_003298"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HDV2813889.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQHARKVA"
BASE COUNT         2617 a         3245 c         2719 g         2701 t
ORIGIN      
        1 ctgctttata attaaaggga caaaataatt ttacatctgt tactgtttcg gtcgcagatt
       61 ctcatcaaat accaggcgtt tcctgccagg ttcaacttcc cgcaatggct cgacttgatg
      121 caacgtttgc ttacagcgtg tcaccagcac ctgatactca tgcgtaccct gctttttcca
      181 tgccagctcc tgctcactaa gttcacgaat cgctttcgcc gggctgccga caatcagata
      241 attagctggc atttctgctt tggctttcac aaatgcggat gcaccaacaa tgctgttctc
      301 gccaatcacc gcaccgtcca tcactaccgc gttcattccc actaatgcat tgcggcggat
      361 aatgcagccg tgaaggatag cgctatgacc aatatgtcca tcttctccta caacagtatc
      421 ctgctcggga aaaccgtgca taacgcaatt atcctgaatg ttcgcgccat ctttcaccac
      481 gatacgacca aaatcgccac gcaggctggc attaggccca acgtaaacgc ccttgccgag
      541 aataacatcg ccgatcaata ccgctgtcgg atggacaaag ctctcttctg gcacaaccgg
      601 agtcagacca tctatctggt aaattggcat aacgcctcct gttcaatatt caagccgcca
      661 aaacgttgaa aatacaggct tcccggcgca ggcagttcac cgactgaggt ttcacctttt
      721 tcactaacga acgctaaagc agcaggcgct acgcgctgat aaatgttgat acacagctga
      781 cgcgccgtat gccctgccca gtgagccgga agcaactcct ccggcaacaa tgggtcttta
      841 aggacgacac ggcgataaaa atggatcagt aaaagctgaa tatgaaatgc acgctccggg
      901 gttaactcgt ctgccgtctc ttttaacagc ggcaccagcg ggcggaatga ctgaataaag
      961 gtttcgtaca tggcattttg ttcagttaaa tgccagcact cttctactct ggcacgcagt
     1021 gctgcgcgag aaagcgccag tggtatttgc gcttcaaaac aaatcacgtt atccgccacc
     1081 cccgcttcat gcaaaagtgt ctgtacatcc gccagttttt gcgacggcga tgccatcagg
     1141 ctgggtgcca gtgcgccaaa accttgccag atcaactgct ttttgacatc agccagcgtc
     1201 gctttatcta aaccttccga gagcaacagg agccatttac catcccatgc aggttgctct
     1261 gcgcgataaa ttttactttc tgcccgtcgc gtcaggcgca agcctttatc actgaggcta
     1321 tagaaactgc gtcgcccgat gcgggaaaca tccagccagc cttctttatt aagacgaaac
     1381 aaagcggtgc gcacgaaacg ctcaccaaat cccagccctt ccagcaaagc agccagacta
     1441 ccaagccaga tttcaccacc acgatgggaa agcgaatcac catacagaga ggagatcaaa
     1501 gatgtgccac tgaccggaac agcgtttaca gcatgttgga taaaagtatc aagtttactc
     1561 attcgattca gtctgttgtt attgttgtga tttatgaatc atagcacagg tcgtatcgac
     1621 ttctggccct gacgctttac ctcaccaggg ccagacgaca cgtcaggcac caacaatatt
     1681 gcgcagatca aacacccgac acgccttgcc ttctgaacgc gggatactgc cacagttaac
     1741 gatcatcaca tcggtagaga tccccaccat cgacttaatc cgatggcgca gctggtggca
     1801 tacctggcaa cgctgctcat gcgtcaatgt caggctactt tctttcaact ccactttcac
     1861 agaaagtgaa tcaagatgcc cccggcggtt cacctccagt tggtaatgcg gcgataaatg
     1921 ttcgaatttg acaatctctt cttccagttg tgacggaaag acatttacac cgcgaataat
     1981 caacatgtca tcgctgcgtc cgctgatgcg atccatgcgg cgcatagtcc gcgccgttcc
     2041 tggtaacagt cgggttaaat cacgcgtgcg gtaacgaatg accggcaatg cttctttggt
     2101 cagcgtggtg aataacagtt cgccatgttc accatcggca agcggtgtgc cgtcatgagg
     2161 attaacaatt tcaggataga aatgatcttc ccaaatggtt gggccgtcgg tagtttccag
     2221 acactccatc gccacccccg gccccatcac ttctgacagg ccataaatat ccagtgcggt
     2281 gatccccagg cgacgctcaa tttctttacg catggcctgt gtccacggct cagcaccaaa
     2341 cactccaacc cgcagcgagc aaccgctggc atcaccgccc aactgccgct ccagctcttc
     2401 aatcaggtta aggcaataag atggcgtaac catgatcata tctggttgaa aatcacggat
     2461 cagttgcgct tgtttttctg tctgcccgcc agacatcggg atcaccgtag cgcccagacg
     2521 ttcggcacca tagtgcgcac ccagcccacc agtaaacagc ccgtaaccat aggcgacatg
     2581 aattttgtct ttgggcgtgc cacccgccgc acgcaacgaa cgcgctacaa tattggccca
     2641 gttatcaata tcgttttgcg tatagccgac aactgtcggt ttacctgtgg ttcccgaaga
     2701 agcatgaatg cgcaccactt gttccatcgg cacggcaaag gtgtcgaaag gatagttgtc
     2761 gcgcagatcc tgtttggtgg tacagggaaa tttacgcagg tctgaaagtt ccctgaaatc
     2821 atcaggatgt acgcctgctg cgttgaattt gcgccgatac atcgggacat tttcatacgc
     2881 gtgtttgagc gtccatttca gacgctgtgt ttgtaacgcc tgtaactcat caacagacgc
     2941 ggtttcaatc gggtcaagct ttgtattggt tatcattgta gggtactcgc aggttgatat
     3001 gctcaaacac gctccagaat catggcgatg ccctgaccga caccgatgca catggtgcac
     3061 aatgcgtaac gaccgttacg ccgatgcagc tcatggctgg cagccagtgc caggcgggca
     3121 ccactcattc ccaacggatg gcctaaggcg atagcgcctc cgttggggtt aacatgtggg
     3181 gcatcatcag gcagccccaa ttcgcgtagt acacccaacg cctgggccgc gaatgcttcg
     3241 ttcagttcaa tcacgtccat atcgtgaata ctcagccccg cgcgttccag cacccggcga
     3301 gttgcaggca ccggaccaag ccccatcagg cgcggttcca ccccggcggt tgccatggct
     3361 acgatacgcg ctcgcggtgt cagtccttgc gctgctgcca tctgttcact ggcaataatc
     3421 aacgcagcgg ctccgtcatt caccccggag gcattgcctg cggtgatcac tccattggca
     3481 cgaaatggtg cttttagtcc acgtaactgt tccagcgtcg tttccgggcg caaatgctcg
     3541 tcatgttgta tttctgttac aacacctttc ttatttttca acacaaccgg aacaatctct
     3601 tcagctaaaa tgcctgaaga ttgcgctttt gccgaacgtt gctgactgcg tagcgcaaaa
     3661 ctatcttgat cttctcgcga gatttttaac agttcagcta cattctctgc cgtttccggc
     3721 atgctgtcag ttccaaattg ttgagccatg agcgggttca caaatcgcca gccaatagtg
     3781 gtatcaaaca tctcagcctg acgagaaaat gcactggttg ccttgcccat aacaaacggt
     3841 gcccgagaca ttgactccac gccaccggcg atcagcaaat cgccatcgcc cgctttaatc
     3901 gcccgtgcgg cgaaccccag tgcgtccagc ccggaaccac acaagcggtt aatggttgtg
     3961 ccggaaacac tctgcggcag ccccgccagt aaagtcgcca tccgggctac gttacggtta
     4021 tcttctcccg cctgattagc acagccgagg atcacatcat cgatacactc cgcatcgaga
     4081 cgcgggtttc gcaccagcag ctcccgcaaa gggatagcag ccagatcgtc agcccgaaca
     4141 cctgataatg ccccgccgta gcgaccaatt ggcgtacgaa ttccgtcaca gataaaggct
     4201 tcacgcatca ggcttctcct gtaatggtgc cgccgatgcg gtgagattta ccgcgaaaca
     4261 gcgcaaccgt tttttgttgt tggttaacaa tttcgatgtc gtaaacaccg gtttgcttgc
     4321 cctgatgacg tacctgcgca gtagcagtta aggtgtctcc ggcaaagcct ggacgtaaaa
     4381 aatcaatcgt gcaggcagaa gcgacggctg ccagcccctg gctattgcag gcgtaggcaa
     4441 aggcagtatc agccagtgaa aatagctgcc cgccgtggca actttgatga ccgttaagca
     4501 tttgtgcagt gacggtcatg gtcactacag caaagccttc atccattgag ataatgtcga
     4561 tgccaagcgc tttggcgcag gcatcgttct catacattgc atgggcattt tgccaggcct
     4621 tatgactcat aaccgctctc cagaagcgcc cgttggcgca gcaatgaaca tgggcgatag
     4681 cgttcttcgc catagtgatg ctgtagattt tcaaggagcc ttaatattcg ctgccagcca
     4741 agttgcgctc cccaggcaag tgggccatat ggataattca ccccaagacg catggcggta
     4801 tcgatatcct gttcagaggc cacgcctttt tgaagcgcat caagggcttc attgatgatc
     4861 attgctaccg ttcgccaaat cagcattcct gggtaatctg caatttgcag cactgttttg
     4921 ccctgctgtt gcaggtaata aatggccttg cgggtcgctg agtccgggtt caccgctgca
     4981 gcagcaatgg tcaccacctt gcccgccatt ttatcgatca ctaccaccgg gcgtgccagt
     5041 cgtattgcca gcgcctgtgc cgtctcgcct tgtgtctcaa tcaataaaac atcgtcaatt
     5101 tccgtgacac cgtcactttt cttttctact ttcattgggc taaaactgtc gcttaccgct
     5161 tccaggccaa caactgcctc acgttccgcg cgccagtcgt acacgcccag cccacttttc
     5221 ttgcccaacc gtccaccaat caccagttcc tgttgcacca gcgaaggtaa aaaacgacgc
     5281 tcctgccaga aagcgttaaa caccgaacag gtgacagcaa aattgacgtc ctgaccaatc
     5341 agatcggtta attccagcgg ccccatcggg aaaccagcgc catcgcgaag tgcagcgtca
     5401 atcacttctg gtgcagcaac ctgctcttcc agtgcccgcc aggcctcgga ataataagga
     5461 cgcgcaacac ggttaacgat aaatccagga gtcgaatgac agcgcacagg ctgcttaccc
     5521 caactcaacg ttagttcaca caactgctca acaacttccg ccgccgttgc cagcccactg
     5581 accacctcca ccaacttcat caccggtgcc gggttaaaaa aatgcagccc cgcaacacgt
     5641 tcaggatttt ttatctccgc agcaatcgcg gttatagaga ttgacgaagt gttagtggtc
     5701 aatagcgttt gtggcgggca aacttccgcc agctgtgcaa agagcgcttt tttgacttcc
     5761 agacgttcag acgccgcttc aatgaccagg tccgcagctg ccagcgcgtg aatatcggtc
     5821 accgggatca ggcgtttcaa tgtgcgttca caggtttcag cagtcagttt tccccgcgtc
     5881 acgcgtgaat ttagccgcgc gtgtatcccg tcgattgcgc gggtcagcgc ttcagcagaa
     5941 atgtcataca gtaaaacctg gtgtccatga ctggcagcaa cttcagcaat gcctgccccc
     6001 atggtgccgc tcccaatcac tgccacagtt tgcagattta tcatcatgct atttccccat
     6061 gaactgcggt gagcgtttag ccaggaacgc actgacacct tcacgataat ctgcgctccg
     6121 tccggcaagt cgctgatagt cacgttccag atccagttgc gtatcgagcg tattggtttc
     6181 agcgctattt atcgcttgct tgataagtcc aagaccaaat gtcggttgtg tcgccagatg
     6241 ccgtgccagc tgttgcgcgg tatctgccag cgtttcatca tcaacaacct gccagatcat
     6301 cccccattcg tgcgcctgtt cagcactcag ttgattcccc agcagtgcca gccccatggc
     6361 gcgcgctcgc ccagcaacgc gtggcagtaa ccaggttcca ccgcaatcgg gtactaagcc
     6421 taacttacta aacgccatga cgaattttgc tgaacgggca gcaataacga tgtcgcaccc
     6481 cagtgccagt gttgcgccag cccctgccgc cacgccattg actgcacaga tcaccggttt
     6541 tggcagtttt gccaggcgac gtaccagcgg gttatagaaa cgctccactg acatccctaa
     6601 atccggtgcg gggcctgtgg gatcaacatt acgatcgtta agatcctgac cagcacaaaa
     6661 tccgcgcccg gcacctgtaa ttaacaggca acggatagtg tcgtcgcgct cggcctgttt
     6721 caggctctct gcgagttgtg cgtgcatctc atcattaaaa ctgttcaggc gttccgggcg
     6781 gttgagcgtt agtgtcatca cgcccttttc tacatgactg aggatgaatt ccatcattag
     6841 cgtcctttaa agtcaggcga gcgtttttgt aagaaagcag agatgccttc atgacgatct
     6901 tctgttgccg ccagcaaggt gaataactgt cgctcctggg caagtccagc ttgcaaagcc
     6961 acttcctgcg actggcgcag cgcttgcttt gccgcttgta aggccagcgg cgagtgacgt
     7021 gccattttcg atgccagctg taaggcgtat tcgagggtta aatcgctggg gaatacgtcg
     7081 ctaaccagcc cggcctgctg tgcttgccgg gcggtgatgc tttctccgct cagcaccatt
     7141 ttgctggcta acgatttacc gacactacgg attaaacgtt gcgttccgcc tgcgccaggc
     7201 atgatgccga gagtgatttc cggcaaccca aaacgcgcgt tctctccggc aaccaccaca
     7261 tcgcacaaca atgccagttc gcaacccgcc ccaagcgcgt aaccgttgac ggctgcgatg
     7321 agtggtttat taaaggcctg caatcgcgcc catagctgcg gacgtgtatc gtttaaggtg
     7381 gccgcgagat ctttttccgc catttcgttg agatcggccc cggcggcaaa aaagcgtgca
     7441 ttaccggtaa tcacacagac cgaaatgctg gtatcgatag ccgcagcttc cagctcattt
     7501 accagttgcg tcagcagggc attatttagc gcattacgtg cggcgggacg gttaagggtc
     7561 agcaacaata ctcgttgctg acggctgacg atcagttcgc tcatgccatc cccttcgcgt
     7621 caaagtcaac caccacatcg ctggtcagcg gcagtgcctg gcaactcaac acatagcctg
     7681 cggccagttc atccggttcc agactgtaat tggtttccat cgccactttg ccacgcagca
     7741 ctttgcattt gcaggtcgca cagacgccgc ctttgcaggc atagggcaga tccgccccct
     7801 ggcgcaatgc cgcatcgaga atgctttcat cgtcggcatt aagcacgatt tcccgatccc
     7861 gcccatcctg acgtacagtc actttttgtc cgtcactttg cacgttaacg ctacgtttga
     7921 cgcgcgtgcc aggcgtatta aaccgctcca gatgaatcgc tttttctggc attcccagtg
     7981 cttttaaggc ggtttccgcg tcatccatca tcgccgccgg accacaaata aacgcctcat
     8041 cataaagacg aaaattaatg agcgaggccc caagtgactg taatttttca ccgtcaatac
     8101 gcccgtgaag cagatcgctg tcgagggttt cctgactgaa aatgcacaac aactgtaaac
     8161 gctgaggata tttgtctttc aggtctgcca gtgcctggcg aaacatcatg ctctggctgg
     8221 tacggttacc gtagatcagg gtgaactgac tttcaggctc ggtttgtaaa gtggtggcga
     8281 taatcgccag cattggcgta atacctgatc ctgctgcaat tgccagatag cggccctggc
     8341 gttcggcctg cggctgatag ccgaaatgcc cctgcggcac catgacctcc agcgtcatac
     8401 cctggcggat gtgttcgcgg gcatagcggg agaaacgtcc gccttcaatg gctttcaccg
     8461 ccacactaat ttcgccaggc agatagctgc ggcaaatgga gtaacaacgg cgtaattctt
     8521 caccatcaaa gctggctttt aaggtcaaat gttgaccggg gcgaaagcga tacgcctcct
     8581 gcaaaggctg gggcaccgca aaggtaatgg tcaccgcatc acgggtttcc ggctccactt
     8641 ttgccaccgt taaggaatga aacgttgtca tggcatcctc aaatacattt gaaataatcg
     8701 aaaggttcgc ggcaactatc gcagcgatac aatgctttgc aggccgtgga accaaattca
     8761 ctgataagtg tggtatggac gctggcacag cgcgggcaac gtacttctgg cggcaaatgg
     8821 gcatggcaac tgtgtccggc gggcgggcta atgccatact gtcgcagacg ctcacgggca
     8881 tcgggggtca tccagtcggt ggtccatgcc gggtcgagtt gcagcacaac ctgaacgggg
     8941 gtaaacccgt gggttgtcat tgcctcacgt atcgcgccaa tcaaatgttc cgttgccgga
     9001 caaccggaat aagtcggcgt aaagccgatc acccatcctt ctcccatctg tgtcacatta
     9061 cgcaccatgc ctaaatcggt aatggtcagc accgggatct ccgggtccgg gatctggctg
     9121 agtagcgccc atatctcatg cacctgcggc ggtgcaatgg tggccagacg ttgcataccc
     9181 atctcctctg ttaccactgc tgaccgggca agacacgctg gagatactgc atttctgcca
     9241 gcatgggccc aaggtgttcg gtatgcagtc cttttttacc gccagtgcga tacgcctgct
     9301 cttgcggtac gttcaatgtg gcttcgttga tcccggcaaa aacttcggct tcccacgctg
     9361 cgcgtaaagt gcgtgggtca accgcaatac cctcttcact cagtgcaata tcaatctcgt
     9421 cggcatcgaa cagttcggcg gtaaaacgcc acaacttgtt gatggcctgc tgcatctttt
     9481 gccctgatac gtcagtacca ttgcccagtc gctccagcca gccacgacta aaacgcaggt
     9541 gataacgcgc ttctttaatt gctttggcag aaatcgccgc cagttgcggc tcgcggcttt
     9601 ccatcagacg ggtaaagagc gccacatgcc atgcatcgat gaaatactgg cgtgcaatgg
     9661 tgtcggcaaa attgccgttt ggttgttcaa ccaacaataa gttgctgaac tggcgctcgt
     9721 ctcgggtgaa ggccagggta tcttcatcgc cttctcccgc taattcagcg gcatacgata
     9781 agaagttgcg tgcctgacct aataaatcga ggccaatgtt tgccagtgcg agatcgattt
     9841 ccagttccgg tgcatgaccg caccattccc ccaaccgctg ggagagcacc aggcagttat
     9901 cgcccaggcg caaggtgtaa gccgttaact gattcatttc caacctcaca tgtgctcaat
     9961 gccatcaggg atggagtaaa acgttggatg gcgatagacc ttgctttcag ccggatcaaa
    10021 aaattcaccg cgttcttccg gttgcgaggc aacaatttca ctcgccttca ccacccaaat
    10081 tgaacatcct tcgctacgac gggtgtaagc atcacgggca ttttccagtg ccatccgctc
    10141 atcggcagca tgtaaactgc caacatggcg gtgtgataag ccctgtttgc cacgcacgaa
    10201 aacttcgtat aacggccagt aaacattact cattttgaat ctccttatgc gaccttacgg
    10261 gcatgttgtt tttgtgcatg ggccagcgct gcttcccgta cccaggtacc ttcttcccag
    10321 gctttacgtt tggcgtcgag ccgctcctga ttacaaattc cgcgaccgtt tatcacttcg
    10381 ttaaactcct gccagtcgat ctcaccaaag cggtagtgcc cgctttcagt atcaaaatgc
    10441 agatccgggt caggaacggt cataccgagc atttcaacct gtggaacggt gttatccacg
    10501 aaacgctggc ggagttcgtc gttggtgaaa cgtttgattt tccaggcgag acttctggcg
    10561 ctgtttggcg agttatcatc gtttggcccg aacatcatta aggctggcca ccagaaacgg
    10621 ttaatggcgt cttgcaacat ctgcttttga gcttcgctgc cctgcgccag tgccatgcag
    10681 gcttcaaacc cctgacgctg gtgaaaactc tcttctttgc agattttcac cattgctctg
    10741 gcatacgggc cataagaagt gcggcataac gccacctgat tgacgatcgc tgcgccatcc
    10801 accagccagc cgataacacc gatatcagcc caactcaatg tcggataatt aaagatggag
    10861 gaatatttca tccgtccgtc gagcattttt tggtagatgt cttctctggc gcagcccagt
    10921 gtttcggcgg cgctatagag atacaggcca tgacctgctt catcctgcac tttggctaac
    10981 agaatggctt tacgccgcaa ggttggtgcg cgagtgatcc agttaccttc aggcaacata
    11041 ccaacaatct cggagtgcgc atgctgccca atctggcgga tcaatgtctt tcggtaagca
    11101 tcgggcatcc agtcctgcgg ctcgatagcc gtttcctggg ctatccgttg ctcaaagcgt
    11161 tcttcttggg tcacgtcatc acctttacga ttccagtttt tgtgatttaa ttaaaaacac
    11221 gaatcacgtt gttttgtaaa agttacacaa tggttaacta aaatcacaaa ttttgtatgt
    11281 tt
//
LOCUS       DAPHBJ010000140        11281 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.141, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000140 DAPHBJ010000000
VERSION     DAPHBJ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.141"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            179..1750
                     /gene="garD"
                     /locus_tag="RHZ76_003299"
     CDS             179..1750
                     /gene="garD"
                     /locus_tag="RHZ76_003299"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HDV2813890.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQIAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1899..2234
                     /gene="prlF"
                     /locus_tag="RHZ76_003300"
     CDS             1899..2234
                     /gene="prlF"
                     /locus_tag="RHZ76_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HDV2813891.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2234..2698
                     /gene="yhaV"
                     /locus_tag="RHZ76_003301"
     CDS             2234..2698
                     /gene="yhaV"
                     /locus_tag="RHZ76_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HDV2813892.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2753..3562)
                     /gene="agaR"
                     /locus_tag="RHZ76_003302"
     CDS             complement(2753..3562)
                     /gene="agaR"
                     /locus_tag="RHZ76_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HDV2813893.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3811..5091
                     /gene="kbaZ"
                     /locus_tag="RHZ76_003303"
     CDS             3811..5091
                     /gene="kbaZ"
                     /locus_tag="RHZ76_003303"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HDV2813894.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKVCSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5114..5587
                     /gene="agaV"
                     /locus_tag="RHZ76_003304"
     CDS             5114..5587
                     /gene="agaV"
                     /locus_tag="RHZ76_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDV2813895.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5598..6377
                     /locus_tag="RHZ76_003305"
     CDS             5598..6377
                     /locus_tag="RHZ76_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HDV2813896.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6367..7245
                     /locus_tag="RHZ76_003306"
     CDS             6367..7245
                     /locus_tag="RHZ76_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDV2813897.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7263..7697
                     /locus_tag="RHZ76_003307"
     CDS             7263..7697
                     /locus_tag="RHZ76_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV2813898.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7694..8827
                     /gene="nagA"
                     /locus_tag="RHZ76_003308"
     CDS             7694..8827
                     /gene="nagA"
                     /locus_tag="RHZ76_003308"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDV2813899.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVELDSGLHVQQIWIQSQLASF"
     gene            9178..10332
                     /locus_tag="RHZ76_003309"
     CDS             9178..10332
                     /locus_tag="RHZ76_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HDV2813900.1"
                     /translation="MPKNYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10345..11205
                     /gene="kbaY"
                     /locus_tag="RHZ76_003310"
     CDS             10345..11205
                     /gene="kbaY"
                     /locus_tag="RHZ76_003310"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HDV2813901.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         2703 a         2896 c         2998 g         2684 t
ORIGIN      
        1 taaagcacag atttgcgctt taccttaccg ggcggcactg caatccctga aatgattgac
       61 attgatcaca tttctgcgtt taaactcctg acattcttat ttcacccaat gaagtcattt
      121 atttttaaat gagaccaggt cctcatttta ataacccctg gctggagaat attgcacaat
      181 ggccaacatc gaaatcagac aagaaacgcc aactgcgttt tatataaaag ttcacgacac
      241 agataatgtg gcaattattg ttaatgataa tggcctgaaa gcaggaacgc gttttccgga
      301 tgggctggaa ttaattgaac atattcccca ggggcataaa gtcgcattgc tggacattcc
      361 ggctaatggt gaaattattc gttatggcga agtgattggt tacgccgtgc gtgcaatccc
      421 acgcggaagc tggatcgacg aatcaatggt tgtactaccg gaagcgccgc cgttacacac
      481 gctgccactg gcaaccaaag tcccggaacc cttaccgccg ctggaaggat acacctttga
      541 gggctatcgc aatgccgatg gcagcgtggg caccaaaaac ctgctcggta tcaccaccag
      601 cgtccactgt gtggcaggcg tggtggacta tgtagtaaaa atcattgaac gcgatctgct
      661 accgaaatac ccgaacgtcg atggcgtggt ggggctgaat catttgtacg gttgtggcgt
      721 ggcgattaac gcaccggcgg cagttgtacc tatccgtacc attcacaata tttcgctgaa
      781 tcctaacttt ggcggcgaag taatggtgat tggcctgggt tgtgaaaagt tgcagcctga
      841 gcgcctgctg actggaacgg atgatgtgca agctattcca gtagaaagcg ccagcattgt
      901 cagtttgcag gatgaaaagc atgtcggttt tcagtccatg gtcgaggata ttttgcagat
      961 cgccgaacgc catctacaaa aactgaatca acggcagcga gaaacctgcc cggcttcaga
     1021 actggtcgtt ggtatgcagt gcggtggcag cgatgcgttt tctggtgtaa cggcaaaccc
     1081 ggcggttggc tatgcgtctg atctactggt gcgctgcggc gcaacggtga tgttttcaga
     1141 agtaacggaa gtgcgtgacg cgatccatct gctgacacca cgcgcagtga acgaagaggt
     1201 cggcaaacgg ctgctggagg agatggagtg gtacgataac tatctcaata tgggaaaaac
     1261 cgaccgcagc gccaaccctt cgccgggcaa caagaaaggc ggtctggcaa acgtggtaga
     1321 gaaggcactc ggctccattg ctaaatcggg taaaagcgca attgttgaag tgctgtcgcc
     1381 cggtcaacgc ccgactaaac gcggattaat ttacgccgcg acgccagcca gcgattttgt
     1441 ctgtggcacg caacaggtgg cttcgggtat cacagtgcaa gtgtttacga ccggtcgtgg
     1501 tacgccgtac ggcctgatgg cggtacccgt cattaaaatg gcaacccgca ccgagctggc
     1561 gaaccgctgg tttgatttaa tggatattaa tgcgggcacc atcgctaccg gcgaagaaac
     1621 tattgaagag gtgggctgga agttgttcca ctttattctc gacgtcgcca gcgggaagaa
     1681 gaaaaccttc tcggatcaat gggggctgca taaccagctg gcggtgttta acccggcacc
     1741 ggtgacctga tttcttttgg aacgtccctc gcaaaacatg gccttagtgc catgttttta
     1801 ttgtttaaag cccccacgtc cattaataat gcatttgcat taccttagtt caagcttata
     1861 atttgagcag aaaacaggct gtaaaaggac agtgaatcat gcccgctaat gctcgctctc
     1921 acgctgtact gaccactgaa tcaaaggtca cgatacgcgg acaaacaact atccccgcgc
     1981 cagtgcgtga ggccttaaaa ctgaagccag gccaggacag cattcattac gaaattctgc
     2041 ctggtgggca agtctttatg tgccgactgg gagatgaaca ggaggatcat actatgaatg
     2101 catttttgcg ttttctggat gcagatatcc agaacaaccc gcaaaaaact cgtccattca
     2161 acattcaaca aggaaagaaa cttgtcgctg gcatggacgt caacattgat gatgagattg
     2221 gcgacgacga ataatggatt ttccacaaag ggttaatggt tgggcgctat atgctcatcc
     2281 ctgttttcag gaaacctacg atgctttagt tgccgaagtc gaggcattaa agggaaaaga
     2341 tcctgaaaat tatcagagaa aagctgccac aaagttattg gcggtggtcc ataaagtgat
     2401 tgaggagcat atcacggtca atccatcatc accggcattc cgtcatggca agtcgttagg
     2461 ctctgggaaa aataaagact ggtcacgggt aaaatttggt gctggtcgct atcgtctctt
     2521 ctttcgttac agtgaaaaag agaaagtcat cattctggga tggatgaacg atgaaaacac
     2581 gctgcgcacc tacggtaaaa aaacagatgc ctataccgta ttcagtaaaa tgttaaaaag
     2641 aggacatcct cctgccgact gggaaagcct cacccaagag acagaagaaa accattgatg
     2701 gtgttcacaa tgcccgccga acgtattcgt gttgaccagc gggcaccctt tctcactccc
     2761 cgaccagaat cacttcaacc ccagcctttc gcagtccttc cagactatcc gcaggaatgc
     2821 cttcatcaac aatgatcatg tcgatacgtt gagtatcaat gatcttatgt aaactggaac
     2881 gattgaactt actggaatcg gtgactacga tgatccgttc cgcaacttcg cacatccggc
     2941 ggtttaaacg ggcttcatct tcattatgcg tgctgacgcc gcgctccaga tcgatcgcat
     3001 ctacaccaag aaacagcata tcgaagtggt aattttgcag cgattgttca gcctgatcgc
     3061 cgtaaaaaga ttgcgactga cggcgcaaat gcccgccggt catcagcagc tcaacgcctt
     3121 ccgcttccag caaagcatta gccacgttca taccgttggt catcgcaatt acgtcagtgt
     3181 gcttgcgcat cagacgagca atctcaaaag tggtggtccc ggaatcgagg atcacccgat
     3241 gacctggctg aatcaactca acggcagctt tcgcaacgct gcgtttcatc gcggtgttca
     3301 gtgcgctttt atcttccact gatggctcga ctgacggcgt cgtgctatcg cagatcaacg
     3361 cgccaccata ggcacgcaca gcgatcccct gcttttccag aaacgccaga tcgttgcgga
     3421 tcgtcacggt agatacgcca tacaatgccg acagatcgtt aacctgcaca ctcccttgct
     3481 gtcgcagacg ctgaatgatc tgttctcgtc gctcgctggt gcctgtcact cgcttctcac
     3541 ctgaagcgtc ggtattactc atagtaagtc ctttcgtaaa actttcgttt catttcgttt
     3601 tgcctattaa cgcctttcta ttaagcaaat gcaagcccac cttgcccatt gacgcaagct
     3661 actctcgttt cagtggcttt cattatgttt cttttgtgaa tcagatcaga aaactattgt
     3721 ctttcgtttt atttttatct caccatgacg cagtatcaac tgaaacaaaa cgaaagatta
     3781 atatcgcagt aatctgaact ggagaggaaa gtgaaacatc taacagaaat ggtgagacag
     3841 cacaaagcgg gcaaaacaaa tgcaatttat gccgtttgtt ccgcacatcc gctggtgctg
     3901 gaagctgcaa tccgctacgc cagtgcaaac caaacgccgt tactgattga agcaacctcc
     3961 aatcaggtag accagttcgg cggttatacc ggaatgacgc ccgccgattt tcgcggcttt
     4021 gtttgtcagc tcgccgactc gttgaatttc ccgcaggatg cgttgattct gggtggtgac
     4081 catctggggc caaaccgctg gcaaaacctg ccagccgctc aggcaatggc caatgccgat
     4141 gatttgatta aaagctacgt tgcggcagga ttcaaaaaaa tccaccttga ttgcagcatg
     4201 tcctgtcagg acgatccaat tcccttaact gatgacatcg tggctgaacg cgccgcccgt
     4261 ctggcgaaag tggcggaaga aacctgtctt gaacactttg gcgaagccga tctggagtat
     4321 gtcattggta ccgaagtgcc ggtacctggc ggcgcgcatg aaaccttaag cgagctggcg
     4381 gtcaccacgc cggatgccgc ccgcgccacg ctggaagccc atcgtcacgc ctttgaaaag
     4441 caaggtttga atgccatctg gccacgcatc attgccctgg tggttcaacc cggcgtcgaa
     4501 ttcgatcaca ccaacgttat tgattatcag cccgccaaag cgagcgcctt aagccagatg
     4561 gtcgaaaact acgaaacgct gattttcgaa gcgcactcta ccgattatca aacgccgcaa
     4621 tcgctgcgcc agctggtgat tgaccacttt gccattctga aagttggccc agcgctgacc
     4681 ttcgccctgc gtgaagctct gttctctctg gcggcgattg aagaagaact ggtgccagcg
     4741 aaagtctgtt ctggtctgcg tcaggtgctg gaagacgtga tgctcgaccg cccggaatac
     4801 tggcaaagcc actaccacgg tgacggcaac gcgcgtcgtc tggcgcgtgg ttatagctac
     4861 tcggatcgcg tgcgttatta ctggccggac agccagattg atgacgcttt cgctcatctg
     4921 gtacgtaatc tggcggattc accaattccg ctgccgctga tcagccagta tctgccgctg
     4981 cagtacgtga aagttcgctc cggcgagctg cagccaacgc cacgggaact cattatcaac
     5041 catattcagg acatcctggc gcagtaccac acagcctgtg aaggccaata agcaaaacaa
     5101 agaggaacac gctatgccaa atattgtttt aagccggatt gatgaacgct tgattcacgg
     5161 tcaggtcggc gttcaatggg tcggatttgc gggggcaaat ctggtgctgg tagccaacga
     5221 tgaggttgcc gaagatccgg tacaacaaaa cctgatggaa atggtactgg cagaagggat
     5281 cgccgtacgt ttctggacgc tgcaaaaagt tatcgacaac attcatcgcg ccgccgatcg
     5341 acagaaaatc ctgctggttt gtaaaacacc cgccgatttc ctgacgctgg tgaaaggtgg
     5401 cgttccggtg aatcgcatta acgttggcaa tatgcactac gccaatggca aacaacaaat
     5461 cgccaaaacg gtttctgtgg atgcgggcga tatcgcagca tttaacgacc tgaaagccgc
     5521 tggggtggaa tgcttcgttc agggcgtccc gacagagcct gctgtggacc tctttaaatt
     5581 actttgaggg attcatcatg gaaatcagcc tgttgcaggc atttgcgttg ggcattatcg
     5641 cctttatcgc tggcctggat atgtttaacg gcctaacaca tatgcaccgc ccggtggtcc
     5701 tcggcccgtt ggtcgggctg gtacttggcg atctgcatac cggaatttta accggcggta
     5761 cgctggaact ggtgtggatg gggctggccc cgctggcggg cgcacagccg cctaacgtga
     5821 ttatcggtac tatcgtcggc acggcgtttg ccattactac tggcgtgaaa cccgatgtcg
     5881 cagtcggtgt cgccgtacct ttcgctgtcg cagtacagat ggggattacc ttcctgttct
     5941 cggtgatgtc cggcgtgatg tctcgctgcg accggatggc ggagaacgcc gatactcgcg
     6001 gcattgaacg cgtgaactat ctggcactgc tggcactcgg cactttctat tttctctgcg
     6061 ctttcctgcc tatctacttc ggcgcggaac atgcaaaaac catcattgat gtcctgccgc
     6121 aacgattaat cgacggcctc ggcgtcgcgg gcggcatcat gccagcaatc ggttttgccg
     6181 tgctgctgaa aatcatgatg aaaaacgtct acatccccta cttcatcctg ggctttgttg
     6241 ccgcagcctg gctcaagtta ccggtgctgg ctattgctgc cgctgcgctg gcgatggcgc
     6301 tgatcgacct gctgcgtaaa tctcctgaac cgactcaacc tgcggcacag aaagaggaat
     6361 ttgaagatgg catctaatca aaccaccctg ccgaacgtca gtgaaaacga agaaacattg
     6421 ctgactggcg tcaatgaaaa cgtgtatgaa gatcagagca ttggcgcgga gctgacgaaa
     6481 aaagatatca accgcgtcgc ctggcgttcc atgctgttac aagcgtcttt taactacgaa
     6541 cgtatgcagg cttctggctg gttgtacggt ctgctgcctg ctctgaaaaa gatccacact
     6601 aataaacgcg acctggcgcg cgccatgaag gggcatatgg gtttcttcaa tacccatccg
     6661 tttctggtga catttgttat cggcattatc cttgcgatgg agcgttctaa gcaggacgtt
     6721 aacagtattc agagcaccaa aattgccgtc ggtgcgccgc tcggcgggat tggcgatgca
     6781 atgttctggc taacgctact gccgatttgt ggcgggattg gagccagtct ggcattgcaa
     6841 ggctccattc ttggcgcagt cgtctttatt gtgctgttca acgtggtaca cctggggctg
     6901 cgttttggtc tggcgcatta tgcttaccgc atgggcgtgg cggcgattcc actgattaaa
     6961 gcgaatacca aaaaagtcgg ccatgctgcg tctatcgttg ggatgacggt aatcggtgcg
     7021 ctggtggcaa cctatgttcg tttaagcacc acgctggaaa tcacggcggg cgacgcagtg
     7081 gttaagttac aggctgatgt tatcgacaaa ctgatgccgg ccttcttacc gttggtctac
     7141 accctgacca tgttttggtt ggtacgccgc ggctggagtc cgctgcgtct gattgcagtt
     7201 accgtggttc tcggcatcgt cggtaaattc tgccatttcc tttaaataca atgaggtatt
     7261 agatgttaag tattattttg acagggcatg gcgggtttgc cagcggcatg gaaaaagcaa
     7321 tgaagcaaat tctcggtgag caatcgcagt ttatcgccat cgattttccg gaaacctcaa
     7381 gcaccgcgct gcttacatcg cagcttgaag aggccatcgc ccaactggat tgtgaagacg
     7441 gcattgtttt tctgacggat ttgctgggcg gcacgccgtt tcgcgtagct tcaacgctgg
     7501 cgatgcaaaa accgggctgt gaagtgatca ccggcaccaa tttgcaactg ctactggaga
     7561 tggtgctgga gcgcgaaggg ttaagcggtg aagaattccg tgtacaggca ctggagtgtg
     7621 gacatcgtgg gctgaccagt ctggtggacg agttaggccg ctgccatgaa gaatgtccgg
     7681 tcgaggaagg gatatgacac acgttctgcg cgccagaagg ctgctgactg aagagggatg
     7741 gctcgatgac catcagttgc gtattgctga cggtgtcatc gcagcaatcg aaccgattcc
     7801 agtgggcgtg actgaacgcg atgcggaact gctctgcccc gcttacatcg acacccatgt
     7861 acacggtggt gcgggcgttg atgtaatgga tgacgcgccg gatgtgctcg acaagctggc
     7921 aatgcacaag gcacgcgaag gtgtcggcag ttggttaccg actaccgtaa ccgcgccgct
     7981 taataccatt catgcggcgc tgaaacgtat tgcgcaacgt tgccaacgcg gcggacctgg
     8041 tgcgcaagtg ctggggagtt atctcgaagg accgtacttc acgccgcaga ataaaggcgc
     8101 gcatccgccg gagttgtttc gcgagcttga aattgccgag ctggatcaat tgattgccgt
     8161 ttctcagcac accttacgcg tggtagcgct ggcaccggaa aaagaggggg cattgcaggc
     8221 catccgccat cttaaacagc aaaatgtacg agtgatgctg gggcatagcg cggcgacctg
     8281 gcaacaaact cgcgccgcgt ttgatgctgg tgccgacggc ctggtgcatt gctataacgg
     8341 gatgacaggt ttacatcacc gcgaaccggg aatggttggc gcgggattaa cggacaagcg
     8401 cgcctggctg gaactgatag ccgatggtca tcatgtgcat ccggcggcga tgtcgctgtg
     8461 ttgttgctgt gcaaaagaga gaatcgtgct gatcaccgac gcgatgcagg cagccgggat
     8521 gccggatggt cgctatacgt tatgtggcga agaagtgcag atgcacggtg gcgttgtccg
     8581 taccgcgtcc ggtgggctgg cgggcagtac gctgtctgtt gatgcggcag tgcgcaacat
     8641 ggtcgagttg acgggcgtaa cgcctgcgga agccattcat atggcatcgc tgcatccggc
     8701 gcgaatgctg ggtgttgatg gtgttctggg atcgcttaaa ccgggcaaac gcgccagcat
     8761 cgttgagctg gatagcgggc tgcatgtgca acaaatctgg attcagagtc aattagcttc
     8821 gttttgatag tttgctcctt tattgggcct tcacttcccc cgtaaggcct ttctttttct
     8881 ttcgttttga tctgtgcagc ggtgtcggat gcgacgctaa cgcgtcttat gcgacctacg
     8941 gtacgccgca tccagcaatc ggtgaacata gcgacgtcat tacgcaattc tttaatcttc
     9001 ccttgtcttt tctttcaatg atcacagatt tcgttttatt tcctttttct ccattgaact
     9061 ttcggtttct tttctataga ttttaatcaa cgaaagacat caccaagtga aatgaaacga
     9121 aaggcaagtg aaagcgacaa cgcccgacgt caagttcatc agactaagga ttgagttatg
     9181 ccaaaaaatt acacccctgc tgccgccgca accggtacat ggactgaaga agagatccgc
     9241 catcagcctc gcgcatggat ccgttcactc accaacatcg acgcgctacg ttccgcgctc
     9301 aataacttcc ttgaaccgtt actgcgcaaa gagaatctgc ggatcatcct gaccggagcc
     9361 ggaacctcgg catttatcgg tgacatcatc gcgccgtggc tcgccagcca taccggtaaa
     9421 aacttcagcg ccgtaccgac caccgatctg gtcactaatc cgatggacta cctgaaccca
     9481 gcccatccgc tgctgttgat ctccttcggt cgatccggca acagcccgga aagcgtcgca
     9541 gccgtggaac tggcaaatca atttgtaccg gaatgctatc acctgccgat cacctgcaac
     9601 gaagcgggcg ctctttacca aaacgcgatc aacagcgata acgcgtttgc cctgctgatg
     9661 cccgcagaaa cgcacgatcg cggctttgcg atgaccagca gcattaccac catgatggcc
     9721 agctgcctcg cggttttcgc acctgagacg atcaacagcc aaaccttccg cgacgtggcg
     9781 gatcgttgcc aggcgatcct gacctcactg ggcgatttca gcgaaggtgt gtttggttac
     9841 gcaccgtgga aacggatcgt ttatctcggt agcggtggct tacagggcgc agcacgcgag
     9901 tcggcgctga aagtgctgga actgacggcg ggtaaactgg cggcctttta tgattctcca
     9961 accggattcc gtcatggacc aaaatcgctg gtcgatgacg aaacgctggt ggtggtattt
    10021 gtctccagcc acccttacac ccgtcagtat gatcttgatc tgctggctga acttcgccgt
    10081 gacaaccagg caatgcgtgt aatcgccatc gccgcggaaa gcagcgacat cgtcgctgcc
    10141 ggtccacata tcatcctgcc accgtcacgt cactttatcg acgttgagca ggcattttgc
    10201 ttcctgatgt acgcccagac gtttgcactg atgcagtcgc tgcacatggg caatacgccg
    10261 gataccccat cagccagtgg caccgttaac cgcgtggtgc aaggcgtaat cattcatccg
    10321 tggcaggcat aagaggatcg cattatgagc attatctcca ctaaatatct gttacaggac
    10381 gcccaggcca atggctacgc ggtgcctgct tttaacattc ataacgccga gacgatccaa
    10441 gcgatcctcg aagtgtgcag tgaaatgcga tcgccggtga tcctcgccgg aacgccgggg
    10501 acctttaaac acatcgcgct ggaagagatc tacgccctgt gtagcgccta ttccacaacc
    10561 tacaacatgc cactggcgct gcatctcgac caccacgaat cgctggatga tattcgccgt
    10621 aaagtccacg caggtgtgcg cagtgcgatg atcgacggca gccacttccc gtttgccgag
    10681 aacgtgaagc tggtgaaatc ggttgttgac ttctgccact cacaagattg cagcgtggaa
    10741 gcagaactgg gccgcctggg cggtgttgaa gatgacatga gcgttgacgc cgaaagtgca
    10801 ttcctgaccg atccacaaga agctaaacgc tttgtcgaac tgactggcgt cgacagcctg
    10861 gcggtagcga ttggtacggc gcacggctta tacagcaaaa cgccgaagat tgatttccag
    10921 cggctggcgg aaattcgtga agtggtggat gttcctctgg tgctgcatgg tgccagcgat
    10981 gttccggatg aatttgtccg tcgcactatt gaacttggcg tcacaaaagt gaacgttgcc
    11041 acagaattaa aaatagcctt cgctggcgcg gttaaagcct ggtttgcgga aaatccgcag
    11101 ggtaatgatc ctcgttatta tatgcgcgtc ggaatggatg cgatgaaaga agttgtcaga
    11161 aataaaatta atgtctgtgg ttcagcgaat cgaatttcag cataatcatt agttttttat
    11221 gatttatccc aatgtacttc ccgattaata tcggggagtg ccttaatgga aaaggagata
    11281 a
//
LOCUS       DAPHBJ010000141        11259 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.142, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000141 DAPHBJ010000000
VERSION     DAPHBJ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.142"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            118..1398
                     /gene="wcaK"
                     /locus_tag="RHZ76_003311"
     CDS             118..1398
                     /gene="wcaK"
                     /locus_tag="RHZ76_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HDV2813902.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSATGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTVKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEAK"
     gene            1395..2615
                     /gene="wcaL"
                     /locus_tag="RHZ76_003312"
     CDS             1395..2615
                     /gene="wcaL"
                     /locus_tag="RHZ76_003312"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HDV2813903.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFYADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVIEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADQSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            2626..4020
                     /gene="wcaM"
                     /locus_tag="RHZ76_003313"
     CDS             2626..4020
                     /gene="wcaM"
                     /locus_tag="RHZ76_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HDV2813904.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMNNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANLTNCKFSDLQGDAIEWNVAINDRDILISDHIIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            4178..5173
                     /locus_tag="RHZ76_003314"
     CDS             4178..5173
                     /locus_tag="RHZ76_003314"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HDV2813905.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            5416..6309
                     /gene="galF"
                     /locus_tag="RHZ76_003315"
     CDS             5416..6309
                     /gene="galF"
                     /locus_tag="RHZ76_003315"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HDV2813906.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            6743..7933
                     /locus_tag="RHZ76_003316"
     CDS             6743..7933
                     /locus_tag="RHZ76_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010431973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase"
                     /protein_id="HDV2813907.1"
                     /translation="MGNILQLIGRNKALFGDDVSENEKELQRIVATSRFLVLGGAGSI
                     GQAVTKEIFKRNPQKLHIVDISENNMVELVRDIRSSFGYIDGDFQTFALDIGSVEYDA
                     FIKADGKYDYVLNLSALKHVRSEKDPFTLMRMIEVNILNTEKTIQQSIAAGVKKFFCV
                     STDKAANPVNMMGASKRIMEMFLMRKSEEIAISTARFANVAFSDGSLLHGFNQRLQKQ
                     QPIVAPHDIKRYFVTPQESGELCLMSCIFGENRDIFFPKLNEALHLISFADIAVLYLK
                     QRGYEPHLCETEDEARVLAKTLPAQGKWPCLFTSSDTTGEKDFEEFFTDKEVLDMKRF
                     INLGIIKNDPLYDPVLLDHFKEKIKHMRASLEWSKKDIVKLFFEMIPDFGHKETGKYL
                     DSKM"
     gene            7948..9090
                     /locus_tag="RHZ76_003317"
     CDS             7948..9090
                     /locus_tag="RHZ76_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010431976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LegC family aminotransferase"
                     /protein_id="HDV2813908.1"
                     /translation="MNINSIIEFVRDVYKTNEFIPLHAPVFDGNEKKYVLDTLESTFV
                     SSVGKYVDDFERKMEAYTGTARAVATVNGTAALSAALYLAGVKRGDLVVTQALTFVAT
                     CNALYHLGAEPVFIDVSPVSLGLCPVALESWLSENTELTEHGCQHRTTHQIVRAVVPM
                     HTFGHPVEMDELIAVCKKWRIVLVEDAAESLGSFYKGLHTGTLGEYGALSFNGNKIIT
                     TGGGGMVFCRASEEGVRAKHVTTTAKVPHPYEFYHDEPGFNYRMPNLNAALGCGQMER
                     LDIFLKQKRTLAQRYQTFFEGSEFKFVKEPQYAQSNYWLNAVICENLDSRDAILAQMN
                     EAKVMTRPIWKLMHRLPMFEHAMRDDLKNSEQIEARLVNLPSSPVE"
     gene            9096..10259
                     /gene="neuC"
                     /locus_tag="RHZ76_003318"
     CDS             9096..10259
                     /gene="neuC"
                     /locus_tag="RHZ76_003318"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010431979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HDV2813909.1"
                     /translation="MITTTRKVAVFTGTRAEYGLLYWLMKDIQSDEELQLQLLVSGMH
                     LSPEFGNTWQQIEQDGFSIDEKIEILLSSDSPVGIAKSMGLGILGFADALSRLKPDVL
                     VILGDRFEALAAAQTAMILRIPVFHLHGGEITEGAYDDAIRHAITKLSYLHGTSTEEY
                     KNRVVQLGENPARVTNVGAIGLEHLKRSKFMTVEELSTSLNFSLKKPYVLVTYHPVTL
                     GDEPAEASFTALLDALDKFPELQVILTYPNADDGGRKIIPLLEAYAAKSPERVKAIPS
                     LGQVRYLSAVKYASAVVGNSSSGIIEVPALDVPTVNIGVRQKGRIAAQSVLNCDATTE
                     SITAALTSAISRSYIREGEKISNPYGQGNASAKIIEMIKSMNFEPSKTFYDIK"
     gene            10266..>11259
                     /gene="neuB"
                     /locus_tag="RHZ76_003319"
     CDS             10266..>11259
                     /gene="neuB"
                     /locus_tag="RHZ76_003319"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010431983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HDV2813910.1"
                     /translation="MTLIIAEAGVNHNGDEKLAFKLVDAAHKAGADIVKFQTFKAKNL
                     VTAEAVQADYQVANTKKQESQLEMLSRLELSWEAHHKLVSYCNKLGIEFLSTAFDSES
                     LDFLVNELGVKRLKLPSGELTNAPLVLEHARTGCDIIVSTGMATLAEIEAALGVIAFG
                     FTAPEEAVPSIEAFQRAYSSEVGQKALKEKVIVLHCTTEYPAPVEEINLRAMDTLRQA
                     FGLPVGYSDHSEGIMIPVAAVARGAVVIEKHFTIDKNMEGPDHKASLEPVELEAMIAA
                     IHQIEKALGNSIKAPTVSEIKNKSVARKSLVAAKTIIAGESFTSDNLAIKRPGTG"
BASE COUNT         3059 a         2503 c         2808 g         2889 t
ORIGIN      
        1 cggttctgtg ctcggatctt gtaggtctga taagacgcgt tagcgtcgca tcaggcaaaa
       61 aacggcaacg aaaacaacaa acacagcctc acctggcatc aaatttatga ggaaaaaatg
      121 aaattactta ttctgggcaa ccacacttgc ggcaaccgcg gcgacagcgc catcttgcgc
      181 ggcttacttg atgccatcaa catcctcaat ccacacgccg aagtggacgt gatgagccgc
      241 tatccggtca gttcttcctg gctactcaac cgcccggtaa tgggcgatcc gctgttcctg
      301 caaatgaaac aacacaacag cgcgacgggc gttgtcgggc gcgttaaaaa agttctccgt
      361 cgtcgctatc agcatcaggt attgctctca cgcgtcaccg acactggcaa gctgcgtaat
      421 atcgccatcg cccagggatt cactgacttc gtgcgcctgc tgtcaggtta cgacgccatt
      481 attcaggtcg gcggatcgtt ttttgtcgat ctctacggcg tgccgcagtt tgaacacgcg
      541 ctttgcactt tcatggcgaa aaagccgcta tttatgattg gtcacagcgt tggtccattc
      601 caggatgagc aatttaacca actggcgaac tacgtttttg gtcactgcga cgcgctgatc
      661 ctgcgcgaat cggtcagcct tgatttgatg aaacgcagca atatcaccac tgtaaaagtt
      721 gaacatggcg tcgataccgc gtggctggtc gatcaccaca cagaagactt caccgccagc
      781 tatgccgttc aacattggct ggacgttgcc gcacaacaga aaacggtggc aattaccctg
      841 cgcgaactgg caccgttcga caaacgtctc ggcaccactc agcaagcgta tgaaaaagcc
      901 tttgccgggg tggtcaatcg cattctcgac gaaggctatc aggtgattgc gctttccacc
      961 tgtacgggca tcgacagcta taacaaagac gaccgcatgg tggcgctcaa cctgcgccag
     1021 cacatcagcg atcctgcccg ttatcacgta gtgatggatg aactcaacga tctggaaatg
     1081 ggcaaaattc tgggtgcttg tgaactcacc gtcggtacgc gcctgcactc cgccattatc
     1141 tcgatgaact ttgccactcc ggcgattgcc atcaactatg aacataaatc cgccgggatc
     1201 atgcagcagc tgggactgcc agagatggca attgatatcc gtcatttact ggacggcagt
     1261 ttgcaagcga tggttgcgga taccttaggc caacttccgg cgctgaacgc acgacttagc
     1321 gaagccgtca gtcgcgaacg tcagacggga atgcagatgg tgcagtccgt actggaacgc
     1381 attggggagg cgaaatgaag gtcggcttct ttttactgaa atttccgctg tcgtcggaaa
     1441 ccttcgtcct taaccaaatc actgcgttta ttgatatggg atttgaggtg gagattgtcg
     1501 cgctgcaaaa aggcgacaca caaaacaccc acgcggcgtg gacgaaatac aaccttgccg
     1561 ccagaacccg ctggttacag gacgaaccaa cgggcaaagt ggcgaagctg cgccaccgcg
     1621 ccagccagac cttacgcggc attcatcgta aaaatacctg gcaggcgctc aacctcaaac
     1681 gctatggtgc cgagtcgcgg aacctgattt tgtctgccat ttgtggtcag gtcgcaacac
     1741 cattttatgc cgatgtcttt atcgctcatt ttggcccggc gggggtaacc gcagcaaaac
     1801 tacgcgaact gggtgtcatt cgcggcaaaa ttgccactat cttccatggt attgatatct
     1861 ccagtcggga agtgctcaac cattacactc ccgaatatca acaactgttt cgccgtggcg
     1921 acctgatgct accgataagc gatctgtggg ccggaaggct gcaaaaaatg ggctgcccga
     1981 gggaaaaaat cgccgtatcg cgcatgggcg tggacatgac tcgttttagc ccgcgtccgg
     2041 tgaaagcgcc cgcaacgccg ctggaaatca tttccgtcgc acgcttaacc gagaaaaaag
     2101 gcctgcatgt ggcgattgaa gcctgccgtc agttgaaaga gcagggcgtg gcatttcgct
     2161 atcgcatcct cggcattggc ccgtgggaac gacgcctgcg caccctcatc gaacaatatc
     2221 aactggaaga tgtgatagag atgccgggct ttaaaccgag ccatgaagtg aaggcgatgc
     2281 tcgacgacgc ggatgtcttc ctgttgccat cggttacagg tgcggacggc gatatggaag
     2341 gcattccggt ggcgctgatg gaagcgatgg cggtcggcat tccggtggtt tctactctgc
     2401 atagcggaat accggaactg gtggaggccg atcaatccgg ctggctggtg cctgagaacg
     2461 atgctcgcgc actggcgcaa cgactggcgg cgtttagcca actggacacc gacgaactgg
     2521 ctccggttgt caaacgtgcg cgcgaaaaag tcgaacacga ttttaaccag caggtgatca
     2581 atcgagaact cgccagctta ttgcaggctt tatagaggag aacgtatgcc atttaaaaaa
     2641 ctctcccgac gcaccttcct gacggcaagc tcggcgcttg ccttccttca tacccctttc
     2701 gctcgcgcac ttcccgcccg acaaagcgtt aacattaacg actacaaccc acacgactgg
     2761 atcgcctcat ttaaacaagc cttcagcgaa gggcaaacgg tcgtcgtgcc tgccggattg
     2821 gtttgtgaca atatcaacac cggcatcttc attcctcctg gcaaaacgtt acacatcctt
     2881 ggaagcctgc gcggcaatgg cagagggcga tttgtcttac aggacggcag ccaggtgaca
     2941 ggggaggagg gcggcagtat gaataacatc actctggatg tgcgcggctc tgactgcacc
     3001 atcaaagggc tggcgatgag cggctttggc ccggtaacgc agatttatat cggcggcaaa
     3061 aacaaacggg tcatgcgaaa cctgaccatt gataacctta ctgtcagcca cgctaattac
     3121 gccatcttac gccagggatt tcataaccag attatcggtg ccaacctcac caactgtaag
     3181 ttcagcgact tacagggcga cgccattgaa tggaatgtgg cgattaacga ccgcgatatt
     3241 ttgatatccg accacatcat cgagcgcatc aactgtacta atggcaaaat caactggggc
     3301 atcggcatag gccttgcggg aagcacttac gataataatt acccggaaga tcaggcagtg
     3361 aaaaactttg tcgtggcgaa tatcacggga tcggattgtc gacagttgat ccatgttgaa
     3421 aatggtaaac attttgttat tcgtaatatc aaagcccgca atatcacgcc ggatttcagt
     3481 aagaaagcag gtattgataa cgctacggtc gctatttacg gttgtgacaa tttcgtgatt
     3541 gataatattg aaatgattaa tagcgccggg atgttaatcg gctatggggt aattaaaggc
     3601 aaatatctct cgataccgca aaatttccga gtgaataata ttcaactgga taacacccac
     3661 cttgcttata aattgcgcgg catccaaatc tctgccggga atgctgtctc ctttgtggcg
     3721 ctgactaaca ttgagatgaa gcgtgcgtcg ctggagttac acaacaaacc gcaacatctt
     3781 tttatgcgaa atatcaatgt gatgcaggaa tcctcagttg gacccgcatt gagcatgaac
     3841 ttcgacatgc gcaaagacgt tcgaggcgtg tttatggcga aaaaagaaac actgctgtct
     3901 cttgcaaatg ttcatgcggt gaatgaaaga gggcaaagct ccgtcgatat cgacaggatt
     3961 aatcaccata ttgttaatgt ggaaaagatt aactttagat tgccggaacg gagggagtag
     4021 atttgcgacc attcataaac ttactggata taatgttctg aaagattata atcctcacac
     4081 taactgttaa agctggggct gggcagcatt gactactcag ggaaggtact catctttatc
     4141 ttgtgtttct tacgtaacaa ccagactaaa gtgatttatg aacgataacg ttttgctcat
     4201 aggagcttcc ggattcgtag gaacccgact acttgaaacg gcaattgctg actttaatat
     4261 caagaacctg gacaaacagc agagccactt ttatccagaa atcacacaga ttggcgatgt
     4321 tcgcgatcaa caggcactcg accaggcgtt agccggtttt gacactgttg tactactggc
     4381 agcggaacac cgcgatgacg taagccctac ttctctctat tatgatgtca acgttcaggg
     4441 tacccgcaat gtgctggcgg ccatggaaaa aaatggcgtt aaaaatatca tctttaccag
     4501 ttccgttgct gtttatggtt tgaacaaaca caaccctgac gaaaaccatc cacatgatcc
     4561 tttcaaccac tacggcaaaa gtaagtggca ggcggaggaa gtgctgcgtg aatggtataa
     4621 caaagcacca acagaacgtt cattaactat catccgtcct accgttatct tcggtgaacg
     4681 caaccgcggt aacgtctata acttgctgaa acagatcgct ggcggcaagt ttatgatggt
     4741 gggcgcaggg actaactata agtccatggc ttatgttgga aacattgttg agtttatcaa
     4801 gtacaaactg aagaatgttg ccgcaggtta tgaggtttat aactacgttg ataagccaga
     4861 cctgaacatg aaccagttgg ttgctgaagt tgaacaaagc ctgaacaaaa agatcccttc
     4921 tatgcacttg ccttacccac taggaatgct gggtggatat tgctttgata tcctgagcaa
     4981 aattacgggc aaaaaatacg ctgtcagctc agtgcgcgtg aaaaaattct gcgcaacaac
     5041 acagtttgac gcaacgaaag tgcattcttc aggttttgtg gcaccgtata cgctgtcgca
     5101 aggtctggat cgaacactgc agtatgaatt cgttcatgcc aaaaaagacg acataacgtt
     5161 tgtttctgag taatgcagac gagtcagaga aaaaaggacc aggttggact acgattattc
     5221 ggaaagaggt actgattaaa ttatcagtat gttgatttcc gagtaatccg agtaacccag
     5281 atgctgatgg tgtgtagtgt tatcgtaagg tttaatcaaa gttatgcgga actaagcttt
     5341 ggtatatagc cataccgttt ggctaaccta tggcaataat ttacgcacta tcagccactt
     5401 agaggtagga aaagaatgac gaatttaaaa gcagttatac cggtagcagg tcttgggatg
     5461 catatgttgc ctgccactaa ggcgattccc aaagagatgc taccgatcgt cgacaagcca
     5521 atgattcagt acattgttga cgagattgtg gctgcaggga tcaaagaaat cctcctggta
     5581 actcacgcgt ccaagaacgc ggtcgaaaac cacttcgaca cctcttatga attagaatct
     5641 ctccttgaac aacgtgtgaa gcgtcagctg ctggccgaag tacagtccat ttgcccgccg
     5701 ggcgtgacta ttatgaacgt gcgtcagggc gaacctttag gtttgggcca ctcaattttg
     5761 tgtgcacgac cagccattgg cgacaaccca tttgtcgtgg tactgccaga cgtagtgatc
     5821 gacgatgcaa gcgccgaccc gctgcgctac aaccttgctg ccatgattgc gcgcttcaat
     5881 gaaacgggcc gtagccaagt gctggcaaag cgtatgccgg gtgacctctc tgaatactcc
     5941 gtcatccaga ccaaagaacc gctggaccgt gaaggcaaag tcagccgcat tgttgaattc
     6001 atcgaaaaac cggatcagcc acaaacgctg gactcagaca tcatggccgt aggtcgctat
     6061 gtgctttctg ccgatatttg gccggaacta gaacgcactc agccaggtgc atgggggcgt
     6121 attcagctga ctgatgccat tgccgaactg gcgaaaaaac agtccgttga tgcaatgctg
     6181 atgactggtg acagctacga ctgcggtaaa aaaatgggct atatgcaggc gtttgtgaag
     6241 tatggactgc gcaacctgaa agaaggggcg aagttccgta aaggtattga aaagctgtta
     6301 agcgaataat aaaaatctgg ccgaatgtaa cggttgataa gaaaattata acggcggtga
     6361 agattcgtgg cgaaagtaat ttgttgcgaa tattcctgcc gttattttat ataaacaatc
     6421 agaataacaa cgagttagca ataggatttt agtcaaagtt ttccaggatt ttccttgttt
     6481 ccatagctga ttggtaagac aattagtgtt tgaatttttc aggtttagcg cgagtgtgta
     6541 acgctcgtca catcgtaggc atgtacgcag tgctctggta gctataaagc caggggcggt
     6601 agcgtgtgca aatctgagtt aaggcagcga ttgtgggggc tataggtatg tatagctgct
     6661 cgttctgtag atatgcttga accagagcag cttctgactc aagcatgtcg tcgcaataat
     6721 atttaatcaa cgggatcgta aaatgggtaa tattctccaa cttattggac gcaataaagc
     6781 attattcggt gatgatgtat cagaaaatga aaaagaatta caaagaattg tcgctacatc
     6841 ccgttttttg gttctgggcg gtgcaggctc tatcgggcag gctgttacaa aagagatatt
     6901 taaacgtaat cctcaaaagc tccatattgt cgatattagt gaaaataata tggttgagct
     6961 ggtacgagat atacgtagct ctttcggtta tattgatggg gatttccaaa cctttgctct
     7021 ggatattggt tcggttgagt atgacgcatt tatcaaagct gatggaaaat acgattatgt
     7081 cctgaatttg tcagcgctta agcatgttcg cagtgaaaaa gatccgttta ctttaatgcg
     7141 tatgattgaa gtaaacatcc ttaatacaga gaaaactatt caacaatcta ttgcagccgg
     7201 tgttaagaaa tttttctgtg tttctaccga taaagccgca aaccctgtga atatgatggg
     7261 ggcttccaag cgtattatgg aaatgttcct gatgcgcaag agcgaagaga tcgctatttc
     7321 tactgctcgt tttgcaaatg tagcattttc agatggctca ctgcttcatg gttttaatca
     7381 gcgactgcag aaacaacagc caattgtggc tcctcacgat atcaagcgct attttgtgac
     7441 tccacaggaa tctggtgagt tgtgtttgat gtcctgtatc ttcggcgaga atcgtgacat
     7501 ctttttcccc aaattaaatg aggcgttgca tcttatctcc tttgccgata tcgctgtttt
     7561 gtatttgaaa cagcgtggct acgaacctca tctctgtgag acggaagatg aggctcgtgt
     7621 gttggcgaaa actttgccag cgcagggaaa gtggccatgc cttttcacat ccagcgacac
     7681 cactggtgaa aaagatttcg aggaattttt taccgacaaa gaggtgcttg atatgaagcg
     7741 ctttattaat ctcggcatta tcaagaatga tccactttac gatcccgtgc ttttagacca
     7801 ttttaaagaa aaaattaagc acatgagggc gtcgctggag tggagtaaaa aagacattgt
     7861 aaaactgttt tttgaaatga ttccagattt tgggcataaa gaaacaggta aatatcttga
     7921 cagtaaaatg taaataggcc tcctcgtatg aatatcaatt caattatcga atttgtccga
     7981 gacgtataca aaacgaatga gtttattcct ttgcatgcgc cagtttttga tggcaatgaa
     8041 aaaaaatatg tattagatac acttgaaagc acgttcgttt caagcgtagg caaatatgtc
     8101 gatgactttg agcgcaagat ggaggcttat actggaacgg cgagagctgt tgcgacagta
     8161 aatggtactg cggcattaag tgctgcgctg tatctagctg gggtgaagcg cggcgactta
     8221 gtggttaccc aagcgctgac ttttgttgca acctgtaatg cactttatca tttgggggct
     8281 gaacctgtat ttatagatgt ctcgcctgtc agtctcggtc tttgccctgt tgcgctggaa
     8341 agctggcttt ctgaaaatac cgagctgact gagcatgggt gccagcatcg tacgacacat
     8401 cagattgttc gcgctgtagt gccaatgcac acttttggtc atcctgttga aatggatgaa
     8461 cttattgcgg tctgcaagaa gtggcgaatt gttctggtag aagatgcggc agaaagcttg
     8521 ggttcctttt ataaggggct gcatactggt acgcttggtg agtatggggc tctgagtttt
     8581 aatggaaata agattatcac tactggtggt ggtggtatgg ttttttgtcg cgcttctgag
     8641 gaaggtgtgc gagcgaagca tgtcaccaca accgccaagg ttccacatcc atatgagttt
     8701 taccacgatg agcctggttt taactaccgt atgcccaacc tgaatgctgc tctgggatgc
     8761 ggacaaatgg aacgattgga tattttttta aagcaaaaac gcacgcttgc ccagcgttat
     8821 caaacattct tcgaagggtc agagtttaaa ttcgttaaag aacctcaata tgctcagtct
     8881 aattactggc ttaatgctgt aatctgtgaa aacttggatt cacgtgacgc tattttggca
     8941 caaatgaatg aagcaaaagt gatgacacgc ccgatatgga aactgatgca ccgcttacca
     9001 atgtttgagc acgcaatgcg ggatgatctt aaaaactctg agcaaattga ggctcgcttg
     9061 gtcaacttgc ctagctctcc tgtggaataa gtgcgatgat aacaacaaca cgcaaggtcg
     9121 cagtttttac cggaactcga gcagaatatg gattactcta ttggttaatg aaggacatcc
     9181 agagcgacga ggagctgcaa ctgcaactgc tcgtcagtgg tatgcacctt tcccctgaat
     9241 ttggcaatac atggcaacag atcgagcagg atggtttttc aattgatgag aaaattgaaa
     9301 ttttgctctc ctctgattct ccggtcggta tagcaaaaag catggggttg gggattctgg
     9361 ggtttgctga tgcattatcc cggctgaaac ctgatgtttt ggtcattctt ggcgatcgtt
     9421 ttgaagctct tgcggcggcg caaaccgcga tgattcttcg tattcctgtt ttccatctac
     9481 atggcggtga aattactgaa ggtgcctacg atgatgctat taggcacgcc atcacgaaat
     9541 tgagttattt acacggtact tccacggaag aatataaaaa ccgtgtcgtt cagttgggtg
     9601 aaaatccggc tcgtgttact aatgtgggtg ctattgggtt agagcatctg aagcgtagca
     9661 agtttatgac ggtcgaagag ttatcaacat cattgaattt ttcgctaaaa aagccgtatg
     9721 tacttgtcac atatcatccg gttactttag gtgatgagcc tgctgaggcg agctttacag
     9781 cattgctgga cgcgcttgat aaatttcccg agcttcaggt tattctgacc tacccgaatg
     9841 cggatgatgg cggcagaaaa attattcctt tacttgaagc ctatgctgct aaatcacctg
     9901 aacgcgtcaa agctatcccg tcacttggac aggtgcgtta tctcagtgcg gttaaatatg
     9961 cttcagcggt ggtgggaaac tcttccagcg gcataattga ggtgcctgct ctcgacgttc
    10021 ctactgtaaa tattggcgtg cgtcaaaaag ggcgtattgc cgcacaaagc gtactcaatt
    10081 gtgatgctac aacggaatct atcactgctg cactaacaag tgcgatttcg cgaagttaca
    10141 taagggaagg agaaaaaata tccaatcctt atgggcaagg gaatgcgagt gctaagatca
    10201 ttgagatgat caaatccatg aattttgaac cgagcaagac attctacgac attaagtgaa
    10261 attatatgac gcttattatt gctgaagccg gtgttaacca caacggcgac gaaaaactgg
    10321 cttttaaact tgttgatgct gctcataaag caggtgctga tatcgtcaaa tttcagacgt
    10381 tcaaggcgaa aaacttggta acagctgaag cagtacaggc ggactatcag gttgcgaaca
    10441 ctaaaaagca agaatcacag ttagaaatgc taagtcggtt ggagctttca tgggaagctc
    10501 accacaagct ggttagttat tgtaataaac ttggtatcga attcctttct accgcctttg
    10561 attccgagag cttggatttt cttgttaatg agcttggcgt caaaaggcta aagttacctt
    10621 caggtgaatt gactaacgcg cctttggtac ttgagcatgc gcgcaccggt tgcgacatta
    10681 ttgtctcaac gggaatggcg acattggctg agattgaggc cgcgttgggt gttattgctt
    10741 tcggatttac agcgcctgaa gaggcagtgc cgagtattga agcttttcag cgtgcgtact
    10801 cgtctgaggt tggtcaaaaa gcgcttaagg aaaaggttat cgttcttcat tgcactacgg
    10861 aatatcctgc accagtggaa gagattaatc tacgtgctat ggatacgttg cgtcaggcgt
    10921 ttgggttgcc tgtggggtat tccgatcaca gtgaagggat aatgattcct gtggccgctg
    10981 ttgcacgtgg tgcggttgtg atcgaaaaac acttcacaat tgataaaaac atggaaggac
    11041 cggaccacaa agcatcgctg gagcccgttg aactggaggc aatgattgcc gctattcatc
    11101 agatagaaaa agcgctaggg aatagcatta aagcgcctac tgtgtcagaa atcaaaaata
    11161 agagtgttgc gcgaaaaagc ctagttgctg caaaaactat tatagcgggg gaaagcttca
    11221 catccgataa tctggcaata aagcgccctg gtacaggta
//
LOCUS       DAPHBJ010000142        11195 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.143, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000142 DAPHBJ010000000
VERSION     DAPHBJ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.143"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            22..321
                     /locus_tag="RHZ76_003320"
     CDS             22..321
                     /locus_tag="RHZ76_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HDV2813911.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(441..620)
                     /locus_tag="RHZ76_003321"
     CDS             complement(441..620)
                     /locus_tag="RHZ76_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HDV2813912.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            694..2055
                     /gene="pabB"
                     /locus_tag="RHZ76_003322"
     CDS             694..2055
                     /gene="pabB"
                     /locus_tag="RHZ76_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HDV2813913.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAVKLVNSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIFCSAGGGIVADSQEEAEYQETFDKVNRILKQ
                     LEK"
     gene            2059..2637
                     /locus_tag="RHZ76_003323"
     CDS             2059..2637
                     /locus_tag="RHZ76_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDV2813914.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASAIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2821..4185
                     /gene="sdaA"
                     /locus_tag="RHZ76_003324"
     CDS             2821..4185
                     /gene="sdaA"
                     /locus_tag="RHZ76_003324"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDV2813915.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENSMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4316..5914
                     /locus_tag="RHZ76_003325"
     CDS             4316..5914
                     /locus_tag="RHZ76_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDV2813916.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRVLIKGSPVLIQWTPAASSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDNRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5918..7474)
                     /gene="yoaE"
                     /locus_tag="RHZ76_003326"
     CDS             complement(5918..7474)
                     /gene="yoaE"
                     /locus_tag="RHZ76_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HDV2813917.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            7937..8908
                     /gene="manX"
                     /locus_tag="RHZ76_003327"
     CDS             7937..8908
                     /gene="manX"
                     /locus_tag="RHZ76_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDV2813918.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            8971..9771
                     /gene="manY"
                     /locus_tag="RHZ76_003328"
     CDS             8971..9771
                     /gene="manY"
                     /locus_tag="RHZ76_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDV2813919.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9784..10635
                     /gene="manZ"
                     /locus_tag="RHZ76_003329"
     CDS             9784..10635
                     /gene="manZ"
                     /locus_tag="RHZ76_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDV2813920.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10690..11148
                     /locus_tag="RHZ76_003330"
     CDS             10690..11148
                     /locus_tag="RHZ76_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HDV2813921.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2734 a         2828 c         2834 g         2799 t
ORIGIN      
        1 gttaatttta aggataagaa tatgcctgct gtaatagata aagccctgga tttcattggt
       61 gccatggatg tatcagcgcc aacaccaagt tcgatgaatg aaagcacggc gaagggaatc
      121 tttaaatatt taaaagaact gggagtaccc gcgagtgccg ctgatattac ggcgcgagct
      181 gaccaggaag gctggaaccc tgggttcacg gaaaaaatgg ttggatgggc aaaaaaaatg
      241 gagacaggtg aacgttctgt gattaaaaat cctgaatact tttcaacata tatgcaggaa
      301 gaactaaaag cactggtctg agttaaattt atatcagcat aaatgggtca ggacgctttt
      361 aatcacatat aaaaagcgtc ctttttctca caatcaaatg atcatcggct tctataacga
      421 aatcgatacg gattagtgtg ttactcgtct tcatcctcaa aacgcgccac gattcgctcg
      481 ccggagtggt tggcacgcag ctcttctgcc accagcgcga ttgcctggcc gctgctcatc
      541 ccttgtgcca tcagttcctg gatccgctcg acagcttttt gctgctgttc atgggtgagt
      601 gaaggtaaac ctgcaaacat tgttaactcc tgctaaattg ttggcgctaa ttatttcatg
      661 ctacccggca catagccagt agagtcagga ctgatgaaga cgttatctcc cgctgtgatt
      721 actttaccct ggcgtcagga cgccgctgaa ttttatttct cccgcttaag ccacctgccg
      781 tgggcgatgc ttttacactc cggctatgcc gatcatccgt atagccgctt tgatattgtg
      841 gtcgccgagc cgatttgcac tttaaccact ttcggtaaag aaaccgttgt tagtgaaagc
      901 gaaaaacgca caacgaccac tgatgacccg ctacaggtgc tccagcaggt gctggatcgc
      961 gcagacattc gcccaacgca taacgaagat ttgccatttc agggcggcgc actggggttg
     1021 tttggctacg atctgggccg ccgttttgag tcactgccag aaattgcgga acaagatatc
     1081 gttctgccgg atatggcagt gggtatctac gattgggcgc tcattgtcga ccaccagcgt
     1141 catacagttt ctttgctgag tcataatgat gtcaatgccc gtcgggcctg gctggaaagc
     1201 cagcaattct cgccgcagga agatttcacg ctcacttccg actggcaatc caatatgacc
     1261 cgcgagcagt acggcgaaaa atttcgccag gtacaggaat atctgcacag cggtgattgc
     1321 tatcaggtga atctcgccca acgttttcat gcgacctatt ctggcgatga atggcaggca
     1381 ttccttcagc ttaatcaggc caaccgcgcg ccatttagcg cttttttacg tcttgaacag
     1441 ggtgcaattt taagcctttc gccagagcgg tttattcttt gtgataatag tgaaatccag
     1501 acccgcccga ttaaaggcac gctaccacgc ctgcccgatc ctcaggaaga tagcaaacaa
     1561 gcagtaaaac tggtgaactc agcgaaagat cgtgccgaaa atctgatgat tgtcgattta
     1621 atgcgtaatg atatcggtcg tgttgccgta gcaggttcgg taaaagtacc agagctgttc
     1681 gtggtggaac ccttccctgc cgtgcatcat ctggtcagca ccataacggc gcaactacca
     1741 gaacagttac acgccagcga tctgctgcgc gcagcttttc ctggtggctc aataaccggg
     1801 gctccgaaag tacgggctat ggaaattatc gacgaactgg aaccgcagcg acgcaatgcc
     1861 tggtgcggca gcattggcta tttgagcttt tgcggcaaca tggataccag tattactatc
     1921 cgcacgctga ctgccattaa cggacaaatt ttctgctctg cgggcggtgg aattgtcgcc
     1981 gatagccagg aagaagcgga atatcaggaa acttttgata aagttaatcg tatcctgaag
     2041 caactggaga agtaagacgt ggaataccgt agcctgacgc ttgatgattt tttatcgcgc
     2101 tttcaacttt tgcgcccgca aattaaccgg gaaaccctaa atcatcgtca ggctgctgtg
     2161 ttaatcccca tcgtccgtcg accgcaaccg gggttgttgc tgactcagcg ttcgattcat
     2221 ctgcgtaaac acgctggaca agtggcattc cctggaggtg cagtcgatga cacggacgca
     2281 tcagctatcg ccgccgcgct gcgcgaagct gaagaagagg tcgctatacc gccttccgcc
     2341 gttgaagtta tcggcgtgct gccgcccgtc gatagcgtca ctggctacca ggtaacccca
     2401 gtggtcggca ttatcccgcc cgatctgccg tatcgcgcca gtgaagatga agtctcggcg
     2461 gtgtttgaaa tgccgctcgc ccaggcatta catctgggtc gttatcaccc tttagatatc
     2521 taccgccgtg gtgattcaca tcgggtatgg ctgtcctggt acgaacagta ttttgtatgg
     2581 ggaatgaccg caggcataat tcgtgagctg gcgctgcaaa ttggtgtgaa accctgacta
     2641 tacttatctt tacatctaca aaacactact tgagacaatc atcgcaatat tagttaaatc
     2701 gcggtttttg attagtttaa ttcatgtgaa tagttaagcc agtcgccgcg ttccctctta
     2761 cactatgcgc tgttattagt tcgttactgg aagtccagtc accttgtcag gagtattatc
     2821 gtgattagtc tattcgacat gtttaaggtg gggattggtc cctcatcttc ccataccgta
     2881 gggcctatga aggcaggtaa acagttcgtc gatgatctgg tcgaaaaagg cttactggat
     2941 agcgttactc gcgttgccgt ggacgtttat ggttcactgt cgctgacggg taaaggccac
     3001 cacaccgata tcgccattat tatgggtctt gcaggtaacg aacctgccac cgtggatatc
     3061 gacagtattc ccggttttat tcgcgacgta gaagagcgcg aacgtctgct gctggcacag
     3121 ggacggcatg aagtggattt cccgcgcgac aacgggatgc gttttcataa cggcaacctg
     3181 ccgctgcatg aaaacagtat gcaaatccac gcctataacg gcgatgaagt cgtctacagc
     3241 aaaacttatt attccatcgg cggcggtttt atcgtcgatg aagaacactt tggtcaggat
     3301 gctgccaacg aagtgagcgt gccgtatccg ttcaaatctg ccaccgaact gctcgcgtac
     3361 tgtaatgaaa ccggctattc gctgtctggt ctcgctatgc agaacgaact ggcgctgcac
     3421 agcaagaaag agatcgacga gtatttcgcg catgtctggc aaaccatgca ggcatgtatc
     3481 gatcgcggga tgaacaccga aggtgtactg ccaggcccgc tgcgcgtgcc acgtcgtgcg
     3541 tctgccctgc gccggatgtt agtttccagc gataaactgt ctaacgatcc gatgaatgtc
     3601 attgactggg taaacatgtt tgcgctggca gttaacgaag aaaatgccgc cggtggtcgt
     3661 gtggtaactg cgccaaccaa cggtgcctgc ggtatcgttc cggcagtgct ggcttactat
     3721 gaccacttta ttgaatcggt cagcccggac atctataccc gttactttat ggcagcgggc
     3781 gcgattggtg cattgtataa aatgaacgcc tctatttccg gtgcggaagt tggttgccag
     3841 ggcgaagtgg gtgttgcctg ttcaatggct gctgcgggtc ttgcagaact gctgggcggt
     3901 agcccggaac aggtttgcgt ggcggcggaa attggcatgg aacacaacct tggtttaacc
     3961 tgcgacccgg ttgcagggca ggttcaggtg ccgtgcattg agcgtaatgc cattgcctct
     4021 gtgaaggcga ttaacgccgc acggatggct ctgcgccgca ccagtgcacc gcgcgtctcg
     4081 ctggataagg tcatcgaaac gatgtacgaa accggtaagg acatgaacgc caaataccgc
     4141 gaaacctcac gcggtggtct ggcaatcaaa gtccagtgtg actaatactt cttactcgcc
     4201 catctgcaac ggatgggcga atttatcccc gctttctcgt ctgctgtaat attccccact
     4261 acacttccac tgttgcgtca ggcgtttgtc gccatacgct tacagggtgg cccgcatgca
     4321 aaaagcacaa cggatcatta aaacctatcg ccgtaatcga atgattgttt gtacgatttg
     4381 cgcactcgtt acgctcgctt cgaccctgag cgtgcgattt atttcacagc gtaacttaaa
     4441 tcaacaacgg gtagtacaat tcgccaatca cgctgtagaa gaattagata aagtactgct
     4501 tcccctacag gcaggtagcg aagtcttgct tccgctgatt ggtctgccct gctctgtcgc
     4561 ccatttgcca ttacgtaaac aggcggcaaa actccaaact gtgcgatcca ttggcctggt
     4621 gcaagacggc acactttatt gctccagcat ttttggttat cgcaatgtgc ccgtcgtgga
     4681 cattctggct gaacttcctg caccgcaacc acttttacgc ctgacgaccg accgtgtcct
     4741 gattaaaggc agtccggttt tgattcaatg gacgcctgca gcgagcagta gcaatgctgg
     4801 ggtcatggag atgattaaca tcgacttact gacggcaatg ctccttgagc cacaactgcc
     4861 gcaaatcagt agcgccagcc tgacggtgga caatcggcat ttgctctatg gtaatgggct
     4921 ggtagattcc cttccgcaac ctgaagacaa tgaaaactat caggtttctt cgcaacgctt
     4981 tccttttacc attaacgtta atggtccggg ggctacggcg ctggcatggc actatcttcc
     5041 aacacaatta ccgctggcgg tgctgctaag tttactggtg ggctacatcg cctggctggc
     5101 gaccgcttac cggatgagct tttcccgcga aatcaatctg ggcctggcgc aacatgagtt
     5161 cgaattgttc tgtcagcctt tgcttaatgc gcgcagccag caatgtattg gtgtagagat
     5221 tttgctgcgc tggaacaatc cgcgtcaggg ctggatttca ccggatgtgt ttattcctat
     5281 cgcggaagaa catcatttaa ttgtgccact gacccgctat gtgatggcag aaaccattcg
     5341 tcagcgccat gttttcccga tgagtagtca gtttcatgtt ggcattaacg tcgcacccag
     5401 ccattttcgc cgtggtgtgc tgataaaaga tctcaatcag tactggttta gcgctcaccc
     5461 gattcagcaa ctgatcctcg aaatcaccga acgcgatgcc ttactggatg ttgattatcg
     5521 gattgcccgc gagctacatc gtaaaaacgt caaactggcg attgatgact tcggcaccgg
     5581 caacagttca ttttcctggc ttgaaacatt acgtcctgac gtgctgaaaa ttgataagtc
     5641 atttaccgca gctataggtt ctgacgcggt taactcgacg gtgaccgata tcatcatcgc
     5701 gctggggcaa agactgaata ttgaactggt ggcggagggc gtggaaacgc aagaacaggc
     5761 gaagtatttg cgccgtcatg gcgtgcatat tttgcaaggg tatttgtacg cacagccgat
     5821 gccgctacgt gattttccca aatggctggc gggcagccaa ccgccgcccg cccggcataa
     5881 tggacatatc acgcccatta tgccgttacg ttaatgctta ctcatcttca tcgtgcgccg
     5941 gttgctcttt aacaatgcga accagatcaa cacgataatc attggcttca atgatggtga
     6001 tatgcagtgg ccctacatca atcacatcgc ccacacgggg aatgtgacca tttgccgaga
     6061 tcacgaggcc cgcgaccgtc gcgatatcat cgtcatcggc aaggtgctca acatcaagcg
     6121 cctgctgcaa ggcatgcaaa tctgtaccgc cttttaccag ccagccgtca ccatcagtaa
     6181 tgatttccgg cgtttcgtca gcgtccggga attcacccgc aatggcttcc agcacatcca
     6241 gcggcgtgac cagaccttgt accacaccaa actcgttggt cacgataaca aagctcccgc
     6301 gagcacgacg cagcacgccc aacaggttga tcggatcgag ggtttccggg acgataatcg
     6361 ccggagacgc cgaagcaatc gccgccacat caacgccctc ttccagcgcc accagcagtt
     6421 ctttagcacg tacaataccg atgatttcat ccagttcacc gcgacatacc gggaacagac
     6481 tgtgcggtga agagagcagt tgctcgcgga tttcatcgac cccgagatta gcgtcaaccc
     6541 agcttatttc accgcgcggc gtcatgatcc cgcgcagaga acgcgacgcc agcgtcagta
     6601 cgccgttaat catgtaacgt tcttcttcgg caaatgcacc ttccgggatc ggcatcggca
     6661 tcgggttatc ggcatcgtgc tgaacattgg cctgacgttt cccgcccatc aaacgcagga
     6721 tggcatcggc agtacgcgct cgcagcggca aagtcgactg gtggcgaata aagttgcgac
     6781 gcgcaatctg gttaaacact tcgatgatga tcgagaagcc aatcgcggca tacaggtaac
     6841 ctttcggaat gtggaaaccg aaaccttctg ccaccagact cagaccaatc attaacagga
     6901 agctcagaca gagcaccacc accgtggggt gctggttaac gaatcgcgtc agcggtttgg
     6961 atgccagcaa cataaccgcc atcgcaatca ctaccgccgc catcatcacc ggcagatggt
     7021 taaccatccc tactgcagta attaccgcat ccaacgagaa gacggcgtca aggatgacaa
     7081 tctgtgtgac gaccacccag aaactggcgt agcctttacc gtggccggaa tcatgatcgc
     7141 ggttttccag ccgttcatgc agttcggttg ttgctttgaa cagcaagaat atccccccga
     7201 acaacataat caggtcgcgt ccggagaagg agaaatccat gacggtaaat agcggtttgg
     7261 tcagcgtgac catccatgaa atcagcgaca gcagccccag acgcataatc agcgccagtg
     7321 ataaccccag caaacgcgct ttatcgcgtt gttttggcgg cagtttgtca gcaagaatgg
     7381 cgatgaagac caggttatcg atacccagca caatttcgag aacaacaagc gtgagtagcc
     7441 ccgcccaaat tgaggggtcc attaagaatt ccatgacaag ctcctgctta aggaatagct
     7501 attcgacgcc agaaataatg caggcgtaac gacaaaatgc aaacgaaagg tgcggcatag
     7561 agtgccagaa aggcaggcgt taaaaggcct gatgctgaaa tgacgtcggt gacgatccat
     7621 actgcgggct actgccctat actccatggt tgttaaacgg gagttaaaca tatcagagac
     7681 gcctctgatt tggcaaagat ttaccttcct ttgcaaacga atgtgacaag gatattttac
     7741 ctttcgaaat ttctgctaat cgaaagttaa attacggatc ttcatcacat aaaataattt
     7801 tttcgatatc taaaataaat cgcgaaacgc aggggttttt ggttgtagcc cttatctgaa
     7861 tcgattcgat tgtggacgac gattcaaaaa tacatctggc acgttgaggt gttaacgata
     7921 ataaaggagg tagcaagtga ccattgctat tgttataggc acacatggtt gggctgcaga
     7981 gcagttgctt aaaacggcag aaatgctgtt aggcgagcag gaaaacgtcg gctggatcga
     8041 tttcgttcca ggtgaaaatg ccgaaacgct gattgaaaag tacaacgctc agttggcaaa
     8101 actcgacacc actaaaggcg tgctgtttct cgttgataca tggggaggca gcccgttcaa
     8161 tgctgccagc cgcattgtcg tcgacaaaga gcattatgaa gtcattgcag gcgttaacat
     8221 tccaatgctc gtggaaacgt taatggcccg tgatgatgac ccaagctttg atgaactggt
     8281 ggcactggca gtagaaacag gccgtgaagg cgtgaaagca ctgaaagcca aaccggttga
     8341 aaaagccgcg ccagcacccg ctgccgcagc accaaaagcg gctccaactc cggcaaaacc
     8401 aatggggcca aacgactaca tggttattgg ccttgcgcgt atcgacgacc gtctgattca
     8461 cggtcaggtc gccacccgct ggaccaaaga aaccaatgtc tcccgtatta ttgttgttag
     8521 tgatgaagtg gctgcggata ccgttcgtaa gacactgctc acccaggttg cacctccggg
     8581 cgtaacagca cacgtagttg atgttgccaa aatgattcgc gtctacaaca acccgaaata
     8641 tgctggcgaa cgcgtaatgc tgttatttac caacccaaca gatgtagagc gtctcgttga
     8701 aggcggcgtg aaaatcacct ctgttaacgt cggtggtatg gcattccgtc agggtaaaac
     8761 ccaggtgaat aacgcggttt cggttgatga aaaagatatc gaggcgttca agaaactgaa
     8821 tgcgcgcggt attgagctgg aagtccgtaa ggtttccacc gatccgaaac tgaaaatgat
     8881 ggatctgatc agcaaaatcg ataagtaacg tattgtgttg attatcactc agttttcaca
     8941 cttaagtctt acgtaaacag gagaagtaca atggagatta ccactcttca aattgtgctg
     9001 gtatttatcg tagcctgtat cgcaggtatg ggatcaatcc tcgatgaatt tcagtttcac
     9061 cgtccgctaa tcgcgtgtac cctggtgggt atcgttcttg gggatatgaa aaccggtatt
     9121 attatcggtg gtacgctgga aatgatcgcg ctgggctgga tgaacatcgg tgctgcagtt
     9181 gcgcctgacg ccgctctggc ttctatcatt tctaccattc tggttatcgc aggtcatcag
     9241 agcattggtg caggtatcgc actggcaatc cctctggccg ctgcgggcca ggtactgacc
     9301 atcatcgttc gtactattac cgttgctttc cagcacgctg cggataaggc tgctgataac
     9361 ggcaacctga cagcgatttc ctggatccac gtttcttctc tgttcctgca agcaatgcgt
     9421 gtggctattc cggccgtcat cgttgcgctg tctgttggta ccagcgaagt acagaacatg
     9481 ctgaatgcga ttccggaagt ggtgaccaat ggtctgaata tcgccggtgg catgatcgtg
     9541 gtggttggtt atgcgatggt tatcaacatg atgcgtgctg gctacctgat gccgttcttc
     9601 tacctcggct tcgtaaccgc agcattcacc aactttaacc tggttgctct gggtgtgatt
     9661 ggtactgtta tggcagtgct ctacatccaa cttagcccga aatacaaccg cgtagccggt
     9721 gcgcctgctc aggcagctgg taacaacgat ctcgataacg aactggacta acaggtgagc
     9781 gaaatggttg atacaactca aactaccacc gagaaaaaac tcactcaaag tgatattcgt
     9841 ggcgtcttcc tgcgttctaa cctcttccag ggttcatgga acttcgaacg tatgcaggca
     9901 ctgggtttct gcttctctat ggtaccggca attcgtcgcc tctaccctga gaacaacgaa
     9961 gctcgtaaac aagctattcg ccgtcacctg gagttcttta acacccagcc gttcgtggct
    10021 gcgccgattc tcggcgtaac cctggcgctg gaagaacagc gtgctaatgg cgcagagatc
    10081 gacgacggtg ctatcaacgg tatcaaagtc ggtttgatgg ggccactggc tggtgtaggc
    10141 gacccgatct tctggggaac cgtacgtccg gtatttgcag cactgggtgc cggtatcgcg
    10201 atgagcggca gcctgttagg tccgctgctg ttcttcatcc tgtttaacct ggtgcgtctg
    10261 gcaacccgtt actacggcgt agcgtatggt tactccaaag gtatcgatat cgttaaagat
    10321 atgggtggtg gcttcctgca aaaactgacg gaaggggcgt ctatcctcgg cctgtttgtc
    10381 atgggggcat tggttaacaa gtggacacat gtcaacatcc cgctggttgt ctctcgcatt
    10441 actgaccaga cgggcaaaga acacgttact actgtccaga ctattctgga ccagttaatg
    10501 ccaggcctgg taccactgct gctgaccttt gcttgtatgt ggctactgcg caaaaaagtt
    10561 aacccgctgt ggatcatcgt tggcttcttc gtcatcggta tcgctggtta cgcttgcggc
    10621 ctgctgggac tgtaagactg ttgtacacta ccggggcctt ttggccccgt ttttttatct
    10681 ggaggattaa tgacaatcac ggacctggta ctgattcttt tcatcgccgc actcctggcc
    10741 ttcgcgatct acgatcagtt catcatgccc cgccgtaacg gccccaccct gctggcaatt
    10801 cctttgctcc ggcgtggtcg catcgatagc gttatcttcg tcggattgat tgtcattctt
    10861 atctataaca acgtcacgaa tcatggtgcg ttaataacga catggttatt aagcgcactg
    10921 gctctgatgg gtttttatat attctggatc cgcgttccga agatcatctt taaacaaaaa
    10981 ggttttttct tcgccaatgt ctggattgaa tatagccgaa tcaaagcgat gaacttgtcg
    11041 gaagatggcg tgttggtgat gcaattagaa cagcgtcggc tgttaatccg cgttcgaaat
    11101 atcgacgatc tggaaaaaat ttataagctt ctcgtttcaa ctcaataagt tatgaattta
    11161 gccaaagcta tgtttagtgt atttttaata atcag
//
LOCUS       DAPHBJ010000143        11134 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.144, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000143 DAPHBJ010000000
VERSION     DAPHBJ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.144"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(161..634)
                     /locus_tag="RHZ76_003331"
     CDS             complement(161..634)
                     /locus_tag="RHZ76_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HDV2813922.1"
                     /translation="MTHRKHSHAFGTITLTVTACLLLWGFQLPAWAGDATQVEPLALR
                     KIMQELGRNMQAITGAISQEEWVQVVQLAPKVAAHPEPPLTEKMRILAYLGADATKFR
                     NFDAQTHEAALAMKLAAASSDGKAVTQSFAHVQESCLGCHQAFRKPFVEHFYGAR"
     gene            complement(663..1373)
                     /locus_tag="RHZ76_003332"
     CDS             complement(663..1373)
                     /locus_tag="RHZ76_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019677824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2813923.1"
                     /translation="MSGKGIHIESLSKRYGDGDTAVFALRDVNMHVAPGEVVGLIGPS
                     GSGKSTLLKCLGAVIEPTAGRMTLGDEVIYADGWKVRDLRALRRDKIGFVFQAPYLIP
                     FLDVTDNVALLPMLAGVANGESRAKALELLTALDVQHRARAMPSQLSGGEQQRVAIAR
                     GLVNRPPVILADEPTAPLDSERAMAVIRILNDMARKFETAIIVVTHDEKIIPTFKRIY
                     HIRDGVTHEEAGEGREFE"
     gene            complement(1375..2580)
                     /locus_tag="RHZ76_003333"
     CDS             complement(1375..2580)
                     /locus_tag="RHZ76_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2813924.1"
                     /translation="MISLAGRDILHAWGKFVFTGIGLGLLIGVTLVMAGVYRGMVDDG
                     KALLDNSGADLWVVQKDTLGPYAESSSLNDDVYRAILAMPGVSQAANATYLTMQVRKG
                     ESDVRTMVVGIAPGALGATPGWPPYLVAGRQITRGHYEAVADIATGFKLGDRLTIRRN
                     HYTVVGLTRRMVSSSGDPMVFIPLKDAQEAQFLKDNDAIWQSRRRTEANPVFNRPGDP
                     GLLDAVIASQSSNAFVNAVLVTLKPGHAPDEVAESIQRWKRLTVYTRAQMEDILVGKL
                     IATSAKQIGMFLVILAIVSAAIVAFIIYSLTMDKIREIAVLKLIGTRNRTIAAMIMQQ
                     ALALGVIGFVVGKITATFSAPAFPKYVLLTPMDSVAGFFAVLVICVLASLVAIRIALK
                     VDPAEAIGG"
     gene            complement(2577..3728)
                     /locus_tag="RHZ76_003334"
     CDS             complement(2577..3728)
                     /locus_tag="RHZ76_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDV2813925.1"
                     /translation="MKKLPLQGRTLALLAVIIPLLVLFIYVGLRSGPLAPVAVTVASV
                     ESRAITPALFGIGTVEARYTYKIGPTFAGRVKRLEVHVGDQVKAGQVLGEMEPVDLDD
                     RVRSQESVFKRAEAALREAEARQAYAQTQARRYEQLFAVRSTSEEIVTTKRQELQIAD
                     AALSAAREDIARARSDREALVAQRSNLRLIAPVDGVVAVRDADPGTTIVAGQAVVEVI
                     DPKSLWINVRFDQISASGLAGGLPAHIVLRSRGGQTLKGRVLRVEPKADAVTEETLAK
                     VTFDNKPEPLPPVGELAEVTVDLPALPAAPLIPNAAVQREGDKVGVWQIVDGDLHFSP
                     VKLGTSDLNGYVQVREGLKNGDQVVTYSEKALTARSRIHVVEHIPGVSR"
     gene            complement(3725..4333)
                     /locus_tag="RHZ76_003335"
     CDS             complement(3725..4333)
                     /locus_tag="RHZ76_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDV2813926.1"
                     /translation="MDTHPKHLPADERRAVTVESVVALAGSQNPSEITTAAIAKHMNL
                     TQGALFRHFPNKEAIWQAVMEWVAERLLARIDRSAQGIESPLAAMEAMFMSHIEFVAE
                     HPGVPRMMFGELQRAESTPAKRMVQTLIQRYSERLHRLIEKGKASGELSPSLDNEAAA
                     TLFIGTIQGLVMQSLLAGDVGRMHRDAPRVFAIYRRGIRSAQ"
     gene            4521..5525
                     /locus_tag="RHZ76_003336"
     CDS             4521..5525
                     /locus_tag="RHZ76_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
                     /protein_id="HDV2813927.1"
                     /translation="MENIALIGIDLGKNSFHIHCQDHRGKAVYRKKFTRPKLIEFLAT
                     CPATTIAMEACGGSHFMARKLAELGHFPKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISR
                     LSTLLEDSSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLKRKLDEDEVGQRLLSIPCV
                     GTLTASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLL
                     VQCARVFIQKLEHQSGKLADWVRELLCRKSNFVVTCALANKLARIAWALTARQQTYEA
                     "
     gene            5985..6305
                     /locus_tag="RHZ76_003337"
     CDS             5985..6305
                     /locus_tag="RHZ76_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HDV2813928.1"
                     /translation="MPRPRIPRNICGRPADTCFKPNARPMSQLEHVHLKEDEFEALRL
                     VDLLGMQQQEAAVAMGVSRQTLANVLKAARFKVVDCLTQGKALIMHSEREGVTQDDHS
                     HSSE"
     gene            6280..6741
                     /locus_tag="RHZ76_003338"
     CDS             6280..6741
                     /locus_tag="RHZ76_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013095208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dinitrogenase iron-molybdenum cofactor"
                     /protein_id="HDV2813929.1"
                     /translation="MITAIPVNDDRVANHFTKASHLVLVDERGVELSRTENPALGADC
                     SGKRKLVDLLVQQQVSRVVVRNIGERMLGKLLGHQIAVYQTDCGRRLFTELCDPDTRV
                     LTELNKPEQGRQSFHHEAKGKKCCHSDGNTAENACQGGRQHHHGNGRCCHS"
     gene            7079..7339
                     /locus_tag="RHZ76_003339"
     CDS             7079..7339
                     /locus_tag="RHZ76_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HDV2813930.1"
                     /translation="MTSTLLDAAMTHCLFMQGVQALTAELTVRFISPVCTGDKLMVCA
                     RLLGQRRGIYLLEAWLTKGQQTVARATAKFIVPSQDIALAHR"
     gene            7454..7930
                     /locus_tag="RHZ76_003340"
     CDS             7454..7930
                     /locus_tag="RHZ76_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDV2813931.1"
                     /translation="MSKQIIDIGIAESSRVEIADGLSRLLADTYTLYIKTHYYHWNVT
                     GPMFNSLHLMFENQYNELATAVDDIAERIRSLGCFAPGTYHEFSRLTAVNEDKDIPAA
                     KNMIENLVQGQETVVKTARSLFPLVNNANDEATADLLTQRIQLHEKTAWMLRSLLE"
     gene            complement(8126..8584)
                     /locus_tag="RHZ76_003341"
                     /pseudo
     CDS             complement(8126..8584)
                     /locus_tag="RHZ76_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(8627..10162)
                     /locus_tag="RHZ76_003342"
     CDS             complement(8627..10162)
                     /locus_tag="RHZ76_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDV2813932.1"
                     /translation="MNDTSSDDILLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNLLQQESDTLTGRVDDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPTEPCCPECGGSLSYLGEDAAEQLELMRSAFRVIRTVREKHACRRC
                     DRIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYARQGVVLSRSVLSG
                     WVDACCRLLAPLDEALQHYVLTDGKLHADDTPVPVLLPGNKKTKTGRLWTYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGHIKEAACWAHA
                     RRKIHDVHVRTPSVLTEEALKRIGELYAIESEIRGKRAEERQAVRHQKVLPLLASLEG
                     WLREKQKTLSRHSELAKAFGYALNQWPALIRYAEDGWVEVDNNIAENALRRVSLGRKN
                     WLFFGSDHGGERGASLYSLIGTCKLNGVDPERYLHYVLDVIADWPVNRVGALLPWRVT
                     LPA"
     gene            complement(10212..10559)
                     /gene="tnpB"
                     /locus_tag="RHZ76_003343"
     CDS             complement(10212..10559)
                     /gene="tnpB"
                     /locus_tag="RHZ76_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV2813933.1"
                     /translation="MINLPAGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTPAQLAMLMEGIDW
                     RQPKRLLTSLTML"
     gene            complement(10556..10939)
                     /locus_tag="RHZ76_003344"
     CDS             complement(10556..10939)
                     /locus_tag="RHZ76_003344"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDV2813934.1"
                     /translation="MRAKERLPRKHYSPEFKMELVRLALEEEGSIAALARKHDVNDNL
                     LFKWIRLWQREGRVCRPRKNSSSLPALIPVQLQAGSSLPTFELPSCSPPATCHIKCRG
                     GEITLTHPSAELMSTVLRELMRGPV"
BASE COUNT         2520 a         3038 c         3070 g         2506 t
ORIGIN      
        1 atcagctacc ggtgttgcag tgtagcgagc cacatcgggt gcggtgggac gctgaaaatc
       61 agggccggcg gcgcagccgg ccagaccggc ggtgaccaga ccagctacca ggtaagttgc
      121 cgcaagccgt ctttcgaggc tgatggggcg ctggcatgtt ttagcgtgct ccataaaaat
      181 gctccacgaa aggtttacgg aaagcttggt ggcagcccaa gcagctttcc tgcacgtggg
      241 cgaatgattg ggtcaccgcc ttgccgtcgc tgctcgcagc agccagcttc atggccagcg
      301 ccgcctcgtg agtctgcgcg tcaaagtttc tgaacttggt cgcatcagcg ccgaggtaag
      361 caaggatgcg cattttttca gtaagcggtg gctcggggtg tgcagcaact tttggggcga
      421 gctgaacgac ctgaacccat tcctcctgcg aaatcgcacc ggtaatagcc tgcatgttgc
      481 gcccgagttc ctgcatgatc ttgcgtagcg ccagtggctc aacctgggta gcgtcaccag
      541 cccaagctgg caactgaaaa ccccataaaa gcaggcaggc cgtaacggtc agggtgatag
      601 ttccaaatgc gtggctgtgt ttgcggtggg tcatgaaatt ctcctgtaat tcaatcgttc
      661 tctcattcga actcccgccc ttcgccagct tcctcatggg tcacgccgtc gcggatgtgg
      721 tagatgcgct tgaatgtggg gatgatcttt tcgtcatggg tgacgacgat gatggcggtc
      781 tcgaacttcc gggccatatc gttgaggatg cggattacag ccatggcgcg ctcggaatcc
      841 agcggcgcag tgggttcatc ggccaggatc accggcggac gattgaccag tccgcgcgcg
      901 atggcgaccc gttgctgctc gccaccggag agttgcgagg gcattgcgcg agctcggtgt
      961 tgcacatcaa gcgctgtgag caattccagt gccttcgcgc gcgactctcc attcgcgacg
     1021 cctgcaagca tcggcagcag cgccacgttg tcggtgacat cgagaaacgg aatcaggtac
     1081 ggcgcctgga aaacgaaacc gatcttgtca cgccgtaagg cgcgcaggtc gcggactttc
     1141 cagccatcgg cgtagatcac ttcatcgccc agcgtcatgc gaccggcggt cggttcgatc
     1201 accgcaccca gacacttgag cagcgtgctc ttgccggacc cggaagggcc gatcagtccc
     1261 accacttcac caggcgcaac atgcatattc acgtctctca aggcaaagac agcggtgtca
     1321 ccatcgccat accgcttgct taaactttcg atgtgtatcc ctttgccact catctcaacc
     1381 tccaatggct tcggccggat cgaccttgag tgctatgcga atggcgacga ggctagctag
     1441 aacgcagatc acgagcacgg caaaaaaacc ggcgacagaa tccattggcg tgagcaggac
     1501 atattttgga aaagccggtg ctgaaaaggt ggctgtgatc ttgccgacca cgaagccaat
     1561 cacgcccagg gcgagcgcct gctgcatgat catcgcggcg atggttcggt tgcgtgtgcc
     1621 gatgagcttc aacaccgcga tctcacggat tttgtccatc gtcagcgaat agatgatgaa
     1681 ggcaacgatg gccgcgctaa cgatggccag aatcacaagg aacatgccga tctgcttggc
     1741 cgaggtggcg atcagcttgc cgacgaggat gtcttccatc tgtgcccggg tgtagaccgt
     1801 caaacgcttc cagcgctgga tggattcggc aacctcgtcc ggcgcatgac caggtttcag
     1861 agtgaccagc acggcattga cgaacgcgtt gctgctctgt gaagcaatca cggcatccag
     1921 caaacctgga tcaccgggtc gattgaagac cgggttggct tcggtgcgac gccggctttg
     1981 ccagatggca tcgttgtcct tgaggaactg ggcctcctgg gcatctttga gcggaatgaa
     2041 taccatcggg tcgccgctgg acgacaccat acgccgtgtc agccccacga cggtgtaatg
     2101 attgcggcga atggtaagac gatctcccag tttgaagccg gtggcgatgt cggccacagc
     2161 ctcgtagtga ccgcgcgtga tctggcgtcc agcgacgaga taaggaggcc atccgggtgt
     2221 agcccccaac gcaccgggcg cgatgccgac caccatggtg cgtacatcac tctcgccctt
     2281 gcgtacctgc atggtcaggt aggtcgcgtt cgctgcctgt gagactcccg gcatggcgag
     2341 aatggcgcga tacacgtcat cgttgaggct ggacgactct gcataaggac ccagagtatc
     2401 cttttgcacc acccaaaggt cagcgccact gttgtcgagc aacgccttgc cgtcgtccac
     2461 catgcctcgg tacaccccag ccatgaccag ggtgacgccg atcagcagac ccagaccgat
     2521 gccggtgaag acgaatttcc cccaggcatg gagaatgtcg cggccggcca ggctgatcat
     2581 cgtgaaactc ccgggatatg ctcgaccaca tggatgcggc tgcgcgccgt cagtgccttt
     2641 tcgctatagg tcacaacctg gtccccattc ttgagccctt cgcgcacctg cacgtaacca
     2701 ttgaggtcgg aagtgccgag cttgaccggg gagaaatgca gatcaccatc cacgatttgc
     2761 cagacaccaa ctttatcgcc ttcacgctgg acggcagcgt tggggatcag tggagcggcc
     2821 gggagcgccg gcaagtcaac cgtgacttcg gccagttcac ccactggtgg caaaggttct
     2881 ggtttgttat cgaatgtcac cttggcaagc gtttcctcgg ttaccgcgtc ggccttgggt
     2941 tccacccgca gcacgcgacc tttcaaggtc tggccaccac gcgaacgcag gacgatatga
     3001 gccggcagcc ccccagccag ccccgatgcg ctgatctggt cgaagcgcac attgatccac
     3061 aaactcttgg ggtcgatcac ttccaccacg gcctggcccg cgacgatggt cgtgccggga
     3121 tcggcatcgc gcacggcgac tacaccgtcg accggcgcga tcaggcgcag attgctccgc
     3181 tgcgcgacga gtgcttcgcg gtcggagcgt gcccgggcaa tatcttcccg agcggcggat
     3241 agggcggcat cggcgatctg cagttcctgc cgcttggtgg tgacgatttc ctcgctggtc
     3301 gagcgcaccg caaacaattg ctcatagcgg cgcgcctggg tttgcgcgta ggcttgccgg
     3361 gcttctgcct cgcgcaaagc cgcttccgcc cgcttgaata cagactcctg tgagcgcacc
     3421 cgatcatcaa ggtcgaccgg ctccatctcg ccgagcacct gtccggcctt gacctggtcg
     3481 cctacatgca cctccaggcg tttgacgcgc ccggcaaacg tcggcccgat cttgtaggtg
     3541 tagcgcgcct ccaccgtgcc gatgccgaac agcgccggtg tgatagcccg tgattccacg
     3601 cttgccaccg tcacagcgac cggggcgagc ggccctgatc gcagaccgac ataaataaaa
     3661 agcaccagca aaggaatgat gacggcaagc agtgccaggg tgcggccttg caagggtaac
     3721 tttttcattg cgcactcctt atgccacgcc gataaattgc aaagacgcgc ggcgcatcgc
     3781 ggtgcatacg tcccacatca cccgccaaca gcgattgcat gaccaagccc tggatcgtgc
     3841 caatgaacag cgttgccgcc gcctcgttgt caagcgaggg agacaactcg ccgctggcct
     3901 tgcctttctc gatgagacga tgcaaacgtt cgctgtagcg ctgaatcaag gtttgcacca
     3961 tgcgcttggc cggtgtcgat tcggcacgct gaagctcacc aaacatcatt cttggcacgc
     4021 ctgggtgctc agctacgaat tcgatatgac tcatgaacat cgcctccatg gctgccaagg
     4081 gcgactcgat tccttgtgcg gatcgatcga ttctggctaa taggcgctct gctacccact
     4141 ccatgaccgc ctgccaaatg gcttccttgt tcgggaagtg ccgaaacagc gcaccctggg
     4201 tcaagttcat gtgtttagcg atagccgcag tggttatctc gcttgggttt tgtgaaccgg
     4261 caagcgccac gacggactcg acagttacgg cacgacgttc atcggccggc agatgctttg
     4321 gatgggtgtc cacgagcact ccttgtaaga tagtaattga ttactatctt accacaagag
     4381 gcaactcaaa caagagaaaa cccgacgtac tcgtcaatat ctagagtctg caatgggctt
     4441 aacgtgcttt atttaatgag atggtcactc cctccttccc agtactatgc tgaggacagg
     4501 ctttcattcg gagaaccatc atggaaaaca ttgcgcttat tggtatcgat ctgggtaaga
     4561 actctttcca tattcattgt caggatcatc gtgggaaggc cgtttaccgt aaaaaattca
     4621 cccgaccaaa gctaatcgaa tttctggcga catgcccggc aacaaccatc gcgatggaag
     4681 cctgtggcgg ttctcacttt atggcacgca agctggcaga gttagggcat tttccaaagc
     4741 tgatatcacc gcaatttgtc cgcccattcg ttaaaagcaa caaaaatgac ttcgttgatg
     4801 ctgaagctat ctgtgaagca gcatcacgtc catctatgcg tttcgtgcag cccagaaccg
     4861 aatctcagca ggcaatgcga gctctgcatc gtgtccgtga atccctggtt caggataagg
     4921 tgaaaacaac taatcagatg catgcttttc tgctggaatt tggtatcagc gttccgcgag
     4981 gtgctgccgt tattagtcga ctgagtaccc ttcttgagga cagtagtttg cctctttatc
     5041 tcagccagtt actgctgaaa ttacaacagc attatcacta tcttgttgag cagattaaag
     5101 atctggaatc tcagttgaaa cgaaagttgg acgaagatga ggttggacag cgcttgctga
     5161 gtattccctg cgttggaacg ctgactgcca gtactatttc aactgagatt ggcgacggga
     5221 agcagtacgc cagcagccgt gactttgcgg cggcaacagg gctggtaccc cgacagtaca
     5281 gcacgggagg tcggacgaca ttgttaggga ttagcaagcg gggcaacaaa aagatccgaa
     5341 ctttgttggt tcagtgtgcc agggtattca tacaaaaact ggaacaccag tctggcaagt
     5401 tggccgactg ggtcagggag ttgttgtgtc ggaaaagcaa ctttgtcgtc acctgtgctc
     5461 tggcaaacaa gctggccaga atagcctggg cactgacggc gcgacagcaa acttacgaag
     5521 cataaaggca gaaatacacc agtttaaaca atcattcatc tggttttgcg aatactgata
     5581 ttgatgatac taacggccca ccggcctgtt gaggaacctg taaaacggaa aggctcattg
     5641 aagccgtata ttttctggag gttcatcagg cgcggaactc atcgaggcgc gggaataaaa
     5701 tcccattcag acgccggata gattcaagca agccaacttg tcgtcaaaat cggtgttgca
     5761 aaaacgggag tgaccataga ttccgttttc tgagacgacc cccttatggc agcactttaa
     5821 attgagtctc atacagtatc tgttttcttt ctcttcatct ctcatgacct gctgcccact
     5881 tacagactcc agggtaaagc aaaaaattct ccaccggtga gcagagtata ttgcatatgc
     5941 cagtttatga gtgtatatat agcatatgcc aataaggagc cttcatgcca agacccagaa
     6001 ttccccgtaa catctgtggc cgccctgcgg atacctgttt taagcccaat gcgcgaccga
     6061 tgagccagct tgaacatgtt catttaaaag aagatgagtt tgaagcgtta cggctggtcg
     6121 atttactggg catgcaacag caggaagcgg ctgttgcaat gggggtttca aggcagacac
     6181 tggccaatgt cctgaaagca gcccgtttta aggttgtgga ctgtctcact cagggcaagg
     6241 ccttaataat gcactctgaa agggaaggag ttacacaaga tgatcacagc cattccagtg
     6301 aatgacgacc gggtagcaaa tcattttacc aaagcgagtc accttgtcct ggtggatgag
     6361 cgcggtgtgg agctcagccg gacagaaaat cccgcgctgg gcgcagattg ttcaggtaaa
     6421 cggaagctgg ttgacctgct ggttcaacag caagtcagcc gcgttgtggt ccgtaacatc
     6481 ggggaacgga tgctgggtaa attactgggg catcagatcg cggtgtacca gactgactgt
     6541 ggtcgtcgtt tgttcactga actgtgtgat ccggataccc gtgttctgac tgaactgaat
     6601 aagccggagc agggacgcca gtcctttcat catgaggcga aagggaaaaa atgctgtcac
     6661 tctgacggga acacagcaga aaatgcgtgc cagggtgggc gtcagcatca tcacggaaac
     6721 gggcgctgtt gccattcatg agtttatttc atcacgggcg gtgattttta tcagacccgt
     6781 attgtgtata aaccaggagc cggaatgtgt ggaggaggca gtaatgatgc cgggggagcc
     6841 cctcagctga ctgaattccg gatgcctccc gtcagtacaa ccggacgctg agcagaaaca
     6901 gatgaattgt tatcttgatg ccagaaaggc ccatacctgt tgtatggtct gtagttcccg
     6961 cgacaacaac cctgatacgg tgaacttaat gttttcagag cacccggatg gctctgtatg
     7021 tgcggactac acggccaatc acaggcatca ggggtatacg ggtcttttac atggcggaat
     7081 gacgagtacc ctgctggatg cagcaatgac tcattgtttg ttcatgcagg gtgtgcaagc
     7141 cctgacggct gaactgacgg taagattcat ttcacccgtt tgtaccggag acaaactgat
     7201 ggtatgtgcc agactgcttg ggcaacgcag ggggatttac ctgcttgaag catggctgac
     7261 aaaaggacag cagacagtgg cacgtgccac ggcgaaattc attgttccgt cgcaggatat
     7321 tgctctggcg catcgctagt gtgacagacg acaggtgtac ctggttatta taaaaatttg
     7381 aggcgtgttt tgctcagacc ggaaagaata atcgcatatc gtatattcca ctgaaaccgt
     7441 gtaagaggta gttatgagta aacaaatcat tgatattggt attgctgaat catcccgggt
     7501 ggagattgca gacggcctgt cccggcttct tgctgacacc tataccctgt atataaaaac
     7561 acattactat cactggaatg taactggccc catgtttaac agcttacatt taatgtttga
     7621 aaatcaatac aatgagctgg ccactgccgt tgatgatatc gctgaacgta tacgctcgct
     7681 cggatgcttc gcgcccggca catatcatga attttcacgt ttaacagcgg ttaatgaaga
     7741 taaggatatt ccggcagcaa aaaacatgat tgaaaatctt gtccagggcc aggagaccgt
     7801 ggttaaaact gcacgctccc tgttcccgct cgttaataat gcgaatgatg aagccacagc
     7861 agatctgctt acacaacgta tccagttaca tgagaaaact gcctggatgt tgcgtagcct
     7921 gcttgaataa tgtcaccacg tggcagcaga aaaaacggtt tgtcagccac aggctattac
     7981 tgacagtgca gaatcatccg tgttcctgtg gctgtttttt ctgaccgtca cagtattaaa
     8041 caccattgaa tcagtgggtt aatcagtgaa tgcgtgaacg agaaatacag tccgtgacgg
     8101 gcatcatcgg ctggtattgt agtggtcaag taatattggc cacggtttta cagtaaaaat
     8161 ggtatctgtt ctctgactct tccggcgtca gcccaccgtt ataatggtga ggtctgacgc
     8221 tgttgtagta gttcaatatg taatcattaa tttgctgccg ggcctcgtcc ttgcctgcgt
     8281 aaccattcgt tggcacccat tctgttttca gactgcggaa gaagcgttcc atggggctgt
     8341 tatcccagca gtttccccgc cgactgacgc tttgatttat tctgcaacgc cagagaagtt
     8401 gttgatattt aaggcctgta tactggcttc cctggtcgct atggaacacg acatcccggg
     8461 gttggccacg cgtctcatag gccatccgca gggcactgct tatcagtgcg gtatcggcat
     8521 gcgctgacag actccagccg ataaccctgc gggcaaaaag atccataaca accgccagat
     8581 agcacgtaag cgtgcagtga gaaccgtatt gacgaagtga agctggtcag gcaggcagag
     8641 taacccgcca cgggagcaga gcacccaccc ggttcacagg ccagtcggcg atgacatcaa
     8701 gtacataatg cagatagcgt tccggatcca cgccgtttaa tttgcacgtc ccgataagac
     8761 tgtacagtga cgcaccgcgc tcaccaccat gatccgagcc gaagaacagc cagttttttc
     8821 tccccagact gacccgtcgt agggcgtttt cggcaatgtt attatccacc tccacccagc
     8881 catcttctgc atagcggatc agcgccggcc actggttcag cgcatacccg aacgccttcg
     8941 ccagttctga gtgccttgag agggttttct gtttctcccg cagccacccc tccagtgacg
     9001 ccagcagcgg cagcactttt tggtgccgga ctgcctgccg ttcctctgct cttttacctc
     9061 tgatctccga ctcgatggca tacagctcgc cgatccgttt cagcgcctcc tccgtcagga
     9121 ctgacggggt acggacgtga acatcgtgga tttttcggcg ggcatgggcc cagcacgcgg
     9181 cttcctttat atggccgtcg cggtagagct cgttgaaccc ggcgtaggca tcagcctgca
     9241 gtacgccact gaagcctgca agatgggtct gagggtggat accttttctg tccgggctgt
     9301 aagcgaacca cactgcgggg gccagcgctg atccggcgtt gcggtcgtct cgaacgtacg
     9361 tccataagcg cccggtcttc gtcttcttat tgcccggcaa cagcaccggg acaggcgtat
     9421 cgtcagcatg gagtttgccg tcggtcagga catagtgctg aagggcttca tccagcggtg
     9481 ccagcagacg acaacacgca tccacccagc ccgacagtac agaacgactc aacactacac
     9541 cctggcgggc atagatctcc gactggcgat acagcggtgt gtgctctgca tactttgagg
     9601 tcagcactcg ggccagcagc cccggtccgg cgatcccccg ttcgatgggg cgcgaaggag
     9661 ccggggcctg aacgatgcga tcgcaccgac ggcaggcatg cttttcacgg actgtccgga
     9721 taacccggaa ggcgctgcgc atcagctcca gctgttcggc ggcatcctca cccaggtaac
     9781 tcagcgaacc accgcactcc gggcaacacg gctcagtcgg cagcagccgc ttctcatccc
     9841 gtgggagtga ctcagggaac ggtttgcggg tgcgggtctg acgcagcggg cgctgcactg
     9901 ccgggtcatc cacccggccg gtcagcgtat cgctttcctg ctgcagcagg ttcaggtcag
     9961 cttccatttg cgcgatacgg cgggatacct tttcggaacg actgccgaag ttcatccggc
    10021 gcagcttatc cagttgcgcc tgcagatggt ctatttcgcg ttcccggttg ctcagctttt
    10081 cctgcagggc gtggatcaac gcttcctgtt cggccaggcg ctgtttcagc agaaggatgt
    10141 cgtcagaaga ggtgtcgttc ataagcctgt attttaccag gcttattctg cgacaaccag
    10201 gataaagagc gctacaacat ggtcagggat gtcagtaacc gctttggctg tcgccagtcg
    10261 ataccttcca tcagcatcgc cagttgcgcc ggcgtcagga acactttgcc atcgcgggct
    10321 gagggccagg cgaagcgacc acgttccagt cgctttgtca gcaggcacag accatcgccg
    10381 gtggaccaga gcagttttac ctgactgccg ctgcgtcccc ggaagatgaa gacgtggccg
    10441 gacatcgggt catctttcag cgcggtctgc acctttgcgg cgaggccatt gaagccgttg
    10501 cgcatatcgg tgataccggc aaccagccag atttttgtgc ctgcgggaag atttatcata
    10561 ccggcccccg catcagttcg cgcaggacag tgctcatgag ttcagcagag ggatgagtca
    10621 gtgttatttc tcctccccga cattttatgt ggcaggtagc aggaggagag caggatggta
    10681 gttcgaaggt tgggagagaa gagcccgcct gaagctgcac gggtatcagg gcaggaagcg
    10741 acgatgagtt ttttcggggc cgacagaccc gcccttcacg ctgccagagc cttatccatt
    10801 taaagagcag gttatcattg acgtcatgtt tccgggccag tgcggcaata ctgccttctt
    10861 cttcaagagc cagcctgacc agttccattt tgaattcagg ggaatagtgt ttccggggaa
    10921 gtctttcttt agcgcgcatg aggtgtccac ctgcaaaata agtgggtgct taagttacag
    10981 gatcctggat tcgggaccag acggtactca gacgacgctt actattataa cactatgcat
    11041 aatttttctc ataataaccc cacattgttt actgtcattt aaaaaaatca caatccgaag
    11101 acgtaacatt gagcaaagaa aataattatt tgcc
//
LOCUS       DAPHBJ010000144        11001 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.146, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000144 DAPHBJ010000000
VERSION     DAPHBJ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.146"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(114..458)
                     /locus_tag="RHZ76_003345"
     CDS             complement(114..458)
                     /locus_tag="RHZ76_003345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813935.1"
                     /translation="MQMLSNQRQLTLPHSFKCFPTKGNLHSPIRANAFRTKAPYTVPC
                     EPCFPTKSNFHRSIHTNAFFKRHFSAAIEANAFIQKALFCRHWSKCFYTKGTFLLSIE
                     TNAFHLLDIQFE"
     gene            1108..1461
                     /locus_tag="RHZ76_003346"
     CDS             1108..1461
                     /locus_tag="RHZ76_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pili assembly chaperone"
                     /protein_id="HDV2813936.1"
                     /translation="MELTLNTNVERGKTNAFAFLKSKHAKVPLFILLVLANCSFAYAG
                     SDDGAFGDIWAYMSEALTGAPGKIIACGMLFSVAYFGVVKPNLGLALVSALMMLVMAN
                     GEKIISSFLDAGIPL"
     gene            1480..1830
                     /locus_tag="RHZ76_003347"
     CDS             1480..1830
                     /locus_tag="RHZ76_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer protein"
                     /protein_id="HDV2813937.1"
                     /translation="MRGIFPLSGVFMTSQYEIPPHTYRFPYRINMPLLILFWDAKQLG
                     ITFVTIASGNIFDFFITSVVVAVVFWFAYKKAAEEGIRGKLKHKLWWYGFFPGKSVFS
                     SRYFTDPFIRNLYS"
     gene            1842..2627
                     /locus_tag="RHZ76_003348"
     CDS             1842..2627
                     /locus_tag="RHZ76_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HDV2813938.1"
                     /translation="MKLLSRLKIPFIKNSSNGDFDNKDETSQKGGEEVKGSFLDSKAR
                     FSKEIEGSEIGITYSALIKRDEKLLRVNTVAMIIIGVLVVKNQFLTDPVTIVLPPNMT
                     EEVKVVGNKASESYKTQWALFFSTLIGNINPTNIGFVTTTILDALSPDLQAKTRESLQ
                     QQTNIMQARGVEQSFKPIDMYYDTKNDMVYVWGTKSTRLINVPDKTESSKWTFEWVLG
                     MKNGRPRIAYVNQYSGTPNIKKITINGKEQLATLDNPPPSIGN"
     gene            2630..3862
                     /locus_tag="RHZ76_003349"
     CDS             2630..3862
                     /locus_tag="RHZ76_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HDV2813939.1"
                     /translation="MTSKKVFHCTAITAVILLATSNVVFAEDYQLPATVNNPVVMPVG
                     ADEFQNGVKNAIIKDTGTTTETVQATKPTTALPSLSPASSASPAVNEITGALSNNPTL
                     AGYQQQVKSGNFDSYGRPAGKEPQQNAQSTGSPSKADELYVEARNRYKEVQRVNVPPG
                     GNIVLPVSRGLQNRISTSFKNASVSTSTPADEASIFVNGGDVFISTNTDKPIGIMLSE
                     DSVPESTYNLTLVPLDVPGAMISVTTSLSPTMQAKRETSLDKQNYDEMLARSQSEELT
                     PSDPRQDDHKQRIIDLLTPVALGEVPSGFSLQEDRLSRIPSSEQSPCNFNMYAKLGQR
                     LVGSRELIDVVLVKNDKPYGQVVADQQCITEGVVASALFDKAFLQPGEETELYIVRDK
                     LFKERQTRVTTRPSLIKR"
     gene            3864..4304
                     /locus_tag="RHZ76_003350"
     CDS             3864..4304
                     /locus_tag="RHZ76_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813940.1"
                     /translation="MRKATFLTVISFGLVVFLGTSYYYQTKYIPKGTVNIFPDVHRQG
                     EIVDDAFGKSTIVISDPTLADKFVGSSTESVGRHEVNVIKELGTQNNGSKFVHDESDK
                     SSLTVGDKWPRAGEPYIVPQMTEYERNLKVKRFQQPKNGVNNGH"
     gene            4294..5652
                     /gene="trhB"
                     /locus_tag="RHZ76_003351"
     CDS             4294..5652
                     /gene="trhB"
                     /locus_tag="RHZ76_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein TrhB"
                     /protein_id="HDV2813941.1"
                     /translation="MDIKKAWENKTVRISVIGAALMVLIVVISQSIFTTPVKKEKKTQ
                     KKDMQTGFLIDDSQMNKLSNEESQKTYNEMVRQNRIDQNAAKADRDKAEKAQQENKAQ
                     IASLASQVQQLTTQLTEMQTSRNGNRNLDAGGPRKNVNEQAPAKPYQLNPNAAVNGVS
                     SGYAPISPTRNSPMRTITQSSIKTNGTDGVIQVMPISENRIREGREVVAGSEKAPTRT
                     IRGDGTAPVDSKARHAARKDEMFLPATSIITGVLITGLEAPTSLSSKAEPMPVTMRIK
                     KDIIMPNNYTMDLRDCNLLGSAVGDLASQRAYIRATSISCVNSKGKAFDVIVEAYAVS
                     ENDGKNGIRGNLISRNGNAIAGSAFAGGLSALAGSLSPSKVSSLNIDPNSTAQYQSPN
                     IGALGALAGAGAAQGGLNRLVDYYTSIAEQQWPIVEISPGRPITFVVQKGATIPTNLT
                     SR"
     gene            5661..6173
                     /locus_tag="RHZ76_003352"
     CDS             5661..6173
                     /locus_tag="RHZ76_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV2813942.1"
                     /translation="MENNNDNESPKKSESADSLNIDAAKPVGGIRTTKKVTLEIDIEK
                     TLKYLMVAGLAVLIVIYGYKGGRFVYDNFKAMSQPAYQIAVLDMQTLRKVFNKQNPVL
                     DSQQSKTNFENYFKALMKVYRERGYLVIDASLAVTIPENVEIVSYIDLGGSFGEVQSL
                     PGEPKESEIR"
     gene            6279..7031
                     /gene="htdT"
                     /locus_tag="RHZ76_003353"
     CDS             6279..7031
                     /gene="htdT"
                     /locus_tag="RHZ76_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010892304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide isomerase HtdT"
                     /protein_id="HDV2813943.1"
                     /translation="MEKAKEGGVSTKPLDGVLAKMKSYKPKDAIYIPTGGLYLFQDER
                     SQLMAVTTDGRYTITGGSVMDILQRKSVLSVEDIRKSFFINLDDAPFPLETVASIPLG
                     NPKLKRQAAIFITLDCDGCQDLIKKFYDEREKYRVDIVLIPSPGEPKQELRQLWCSKE
                     KGKVNDLDILRWLMGNKADIEKRLLTKEEAEACPAEPLVASLMLAGIYKLQGVPSVVR
                     QDGLAGNGIPKDFDYWLKQSVEPLLKNPFDTN"
     gene            7041..7991
                     /locus_tag="RHZ76_003354"
     CDS             7041..7991
                     /locus_tag="RHZ76_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraV family lipoprotein"
                     /protein_id="HDV2813944.1"
                     /translation="MNIKKVIFSALVVGSSSYLAGCTIGSAESECPGIEKGVICKGPR
                     EVMELTNNRDDLSGLGEEEANSGKGKSAVNDSQYPAQLTPPGAVQYPKSSVLVNKPVT
                     YTTTEVKPVGQLPVMYDQTLKMGAPTSTIGPRPISGNPVNSNVRMTTSYNSNGSSVNP
                     FLHPSAEVVKKTTQVVTPAPAPRYVAPNSDINASKDLYSINNGQPVNPTLSSGQVQQY
                     RTQGYKQAVVAPEPLAVLQQGRVMRITFAPYTDDNDALNLPGFVYVNVKPQTWIAGKN
                     STSNPARIVPLEVQDAARENMQQQQRATKAVSSNGIVRQL"
     gene            8000..10681
                     /gene="trhC"
                     /locus_tag="RHZ76_003355"
     CDS             8000..10681
                     /gene="trhC"
                     /locus_tag="RHZ76_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer ATPase TrhC"
                     /protein_id="HDV2813945.1"
                     /translation="MRSANVYNKELSRHQLGGFIPVYDQIPGTHYFLLDGNRLGFMFI
                     CSPSPGVFDNQQDVLTELFKMDFPTDTICQTSLTALPDLILHLSAWSAVRGGRMEGHD
                     KLKGDLLTAYQLDYYDRSLNEPLKPDHDKLMLRDFQVWISFSIPLKSALPSEIEKTRI
                     DALYSDLISKLNTVGLFPHKVGAENWLYCMDKLLHPGKTSRWSEGHVEASTMRRLNEQ
                     INVPGRKYTVTENHFSSTTQSNDISEHRYFKQLSVVKFPEFVNFGCMYELVVNWLNGR
                     KTIFSPFMITQTVHFADPLKLSRENVRYKAITNKQASIPTVLTFCPRLKDMDNDYMTI
                     TRELEDGARLLHSYLTFTVMGNSAVDVQSAADQLKSFYLESRVNVADDSYIVFPSFVS
                     SLPMCNDPKTILELDRFEVVSNTGAAHMTPIFGPWKGNTDRPVLNLVSREGQLLGLDI
                     FKTSASYNMVVGATSGAGKSFWVAYIINNYLGAGPRSNNLIHYRDTFEGFKNNSYDAF
                     DPDGAQIFVVDVGRSYQGISEQYTNSQFIDFGKKPDFTLNPFAFLTDVTVGERVFDEA
                     PVFNDDSNNHDDDKDKVAQTIMVLNQLKIMASEKGNIDDFQQSVMLQLISEEYNESRK
                     VGRTGSITGFARRCSNHEDKRIKDIGDQLGQWCEGGIYGNRFTENLPPINFDSRFIVL
                     ELEELKGTPHLQTVVLMSIIQAAQHAMFIKKDGRRRLFILDEAWEYIRPDNASGSSNH
                     SNQFFSSFLEAAWRRFRKTNCAGICITQSFEDYFTSSVGRALTANSPWKIIMKQEKES
                     IEAMKVNNYFSTTDAEYERMKNIRTVKRAFSEMLVRFENFQEICRLYVDRKMELCFTT
                     DSTDRGKLWEIQSRLDCSYGEAIEILYEQEVASKSAA"
BASE COUNT         3329 a         2158 c         2547 g         2967 t
ORIGIN      
        1 tccttaacgc ctgcaaacga ctgggatatg agcctggtcg acggcatttt atgaatatgc
       61 aggccaatga aggacctaga gaaataacat aacattctga taataaatga aaattactca
      121 aattggatat caaggagatg aaaagcattt gtttcaatgg agagcaaaaa agtgcctttt
      181 gtataaaagc atttgctcca atggcggcag aaaagtgcct tttgtataaa agcatttgct
      241 tcaatggcgg cagaaaagtg ccttttgaaa aaagcatttg tatgaatgct gcggtgaaag
      301 ttgcttttgg tcgggaagca gggctcgcaa gggacagtgt aaggtgcctt tgttcgaaaa
      361 gcatttgcac gaataggaga atgcaagttg cctttggtcg gaaagcattt gaatgaatgg
      421 ggaagtgtaa gttgcctttg gttggaaagc atttgcatga atgagtccct gataggtgtc
      481 tttgtttgaa aagcatttgt atgaatggac ccataaaagg agcttttggg caaaaggtag
      541 ttgggtgaat cggagctggg aaagtgcgtt tggccagaaa gcatttgcat gaatggattc
      601 agaagaagtg acttttacga taagcatttg aatgaatggg caccgcagac gcgcattcac
      661 caatcagcat ttgaatgaat gagctccgca gttttgcttt catcgaacag cattcgtatg
      721 aatgtgctcc gcagaggtgg ctttcattga aaagcatttg tatgaatggg ggatacataa
      781 tccttgtggg tgaaaagcac tcgcatcaat gggggaaaag tgacttttgg aatgtcatcc
      841 ttaccgtgtc ctttgttaaa tatctggctg gatgaatgcc ataaaaatgg taaaggggca
      901 gacgatgccc ctcttcccta tttggcatgt taattaaacg caccttcttt aatccgatta
      961 catgcgggat tcttttctga cattcgatgg ctaaaaaaac accaagcgca attaagtcaa
     1021 aaaaaggtgg ttaacagaaa aatgaaagaa aaataaaaag tattatgcca atgcgttttc
     1081 gtgaattcaa atcaacacgg agtaattatg gaactgacat tgaatactaa cgttgaaaga
     1141 ggaaaaacta acgcttttgc attcttgaag tccaaacatg caaaagtgcc tttgtttatt
     1201 ctcttggttt tagcaaattg cagttttgcg tacgcgggtt ccgatgatgg agccttcggc
     1261 gatatctggg cctacatgag cgaagccttg actggtgcgc cgggtaaaat catcgcgtgc
     1321 ggcatgttgt tctcggtggc ttatttcggc gttgtaaaac ctaaccttgg tttggcgctg
     1381 gtatcagcat taatgatgct ggtaatggca aacggtgaaa aaattatcag ctcgttcctg
     1441 gatgctggta ttcctctgtg atttgttaga acaataacaa tgagggggat attccccctc
     1501 tctggagttt ttatgacaag ccagtacgag ataccgccac atacatatcg ctttccatac
     1561 agaataaata tgccgttact aattctattc tgggatgcaa agcaattggg tataacattc
     1621 gtgactatcg cgagcggtaa tatttttgat ttctttataa cttcggtagt tgtggcggtg
     1681 gtgttctggt ttgcatataa gaaagcagca gaggaaggga ttagaggaaa actaaaacac
     1741 aaactgtggt ggtacggctt ttttcctgga aaatcggtgt ttagtagtcg ttactttacc
     1801 gatccattta tcagaaacct ctattcttga tggagctaag aatgaaactt ctcagcaggt
     1861 taaaaattcc attcattaaa aattccagta atggcgattt tgataacaag gatgagactt
     1921 cgcaaaaagg aggtgaagaa gtaaaaggaa gttttctgga ttcaaaagca cgctttagta
     1981 aagaaattga aggttctgaa attggaatta catatagtgc attgattaaa cgcgacgaaa
     2041 aactcctacg tgtgaataca gttgcaatga tcattattgg ggttctggtt gtaaaaaatc
     2101 aatttctcac tgatcctgtg actatagtgc taccgccaaa tatgacggaa gaagtcaaag
     2161 ttgttggaaa taaagcctca gaatcatata aaacgcagtg ggccttattc tttagtactt
     2221 tgattggaaa cataaaccca acaaacatcg gttttgttac aacaactata ttggatgcgc
     2281 tttctccgga tttacaggca aagacccggg aatcactaca acagcaaacc aatataatgc
     2341 aggctcgtgg tgtagagcag tcatttaagc cgatcgatat gtattacgac accaaaaatg
     2401 acatggtcta tgtctgggga acaaagtcaa ctcgtttgat taatgttccg gacaaaactg
     2461 aatcatccaa atggacgttt gaatgggtcc tgggaatgaa aaatggacga ccgcgcatag
     2521 cctatgtgaa ccagtattca ggtacaccaa atattaagaa aattacaatc aacggcaaag
     2581 aacaactggc aacactcgat aatccgccgc cgtcaatagg taactaatta tgacttcaaa
     2641 aaaggttttt cactgcaccg cgattacagc agtgattttg ctggcaactt cgaatgtggt
     2701 atttgctgaa gactatcagt taccggcgac agtaaataat ccggtagtta tgcctgtcgg
     2761 ggccgatgaa tttcagaacg gtgtgaaaaa tgcgataatt aaagacactg gcactacgac
     2821 tgagaccgta caagcaacaa aaccgacaac tgctctgcca agtctttcac ctgcaagcag
     2881 tgcttctccc gcagtcaatg aaataaccgg ggcgttgtca aataacccta ctctagctgg
     2941 ttaccagcaa caggttaaat caggaaattt tgatagttac ggaagaccag cagggaaaga
     3001 acctcaacaa aatgcacaaa gtaccgggtc cccttcaaag gcagatgaac tttatgttga
     3061 agcacgtaat cgctataaag aagtacaacg agtaaacgtt ccgcctggtg gcaatattgt
     3121 actcccggtt tctcgggggc tgcaaaacag aatttcgact tcatttaaaa atgcatctgt
     3181 aagtacttca acgccagctg atgaagccag tattttcgtt aatggcgggg atgtttttat
     3241 ttcaacaaat acagataagc ctataggaat tatgctttca gaagattcag ttcctgaatc
     3301 tacttataac ctgacactgg ttccactgga tgtaccaggg gccatgattt cagtaacaac
     3361 ttctttgagc ccgacaatgc aggcaaaacg agaaacttct cttgataaac aaaattatga
     3421 tgagatgctg gcacgctcac aatcagaaga gttgactcct tcagaccctc ggcaggatga
     3481 ccataaacag cggatcattg atttgttgac tccggttgca ctgggtgagg ttccttctgg
     3541 tttcagttta caagaagacc gcttgtcacg cataccaagc tcagagcagt ccccatgtaa
     3601 tttcaatatg tacgccaagt taggtcaaag actggtggga tctcgggaat tgatagatgt
     3661 cgttcttgtt aagaatgata agccttacgg acaggtagtg gctgatcagc aatgtataac
     3721 tgaaggcgtg gttgcaagcg ctctattcga taaagcattc cttcagcccg gggaagagac
     3781 cgaactttat attgtgcggg ataaattatt taaagagcgt cagactcgtg ttactacgcg
     3841 cccaagcctg attaaaagat aatatgagaa aagctacttt tcttacagtg atctcgtttg
     3901 gtttagttgt ctttttgggt accagttatt actaccaaac taagtatatc ccgaaaggca
     3961 ccgtgaatat atttcctgat gtccaccgac agggtgagat cgttgatgat gcctttggga
     4021 aaagcacgat tgttataagc gacccaacgc ttgcggataa atttgttgga agttcaaccg
     4081 agtctgtcgg tcggcatgaa gttaatgtaa taaaagaatt gggcacgcaa aataatggtt
     4141 cgaaatttgt tcatgatgag tctgataaat caagtttgac ggttggagat aaatggccta
     4201 gggctggtga accttatatc gtgcctcaga tgacagaata cgagcggaat ttgaaagtta
     4261 agcgattcca gcaaccaaag aatggagtta ataatggaca ttaaaaaggc ctgggaaaat
     4321 aaaactgtca gaatctcagt aattggtgct gcattgatgg tattgattgt tgtcatcagt
     4381 caatcaatct ttaccacacc agttaaaaaa gagaagaaaa cccagaaaaa agatatgcaa
     4441 actggattcc ttattgatga ttcacaaatg aacaagctga gtaatgagga aagccagaag
     4501 acttataacg aaatggtaag gcagaaccgt attgaccaga acgctgccaa ggctgaccgt
     4561 gacaaagctg aaaaggctca gcaagaaaac aaagcacaga tagccagtct tgcttcgcaa
     4621 gttcagcaac tcactacgca gttgactgaa atgcagacct caagaaatgg taatcgcaat
     4681 ctcgatgcag gcggaccacg caaaaacgtc aatgaacaag ccccagcaaa gccataccag
     4741 cttaatccaa atgcagcggt gaacggggtt agttctggat acgcacctat atctccgacc
     4801 cgaaacagtc caatgcggac aataacacag agttcaatta agacaaacgg cactgatggt
     4861 gtcattcagg tgatgccgat atcagagaac cggatcagag aggggcgcga ggttgttgcg
     4921 ggaagtgaaa aggccccaac gcgaactatt cgcggcgatg ggactgcacc tgttgatagt
     4981 aaagctcgtc atgcggcccg taaggatgaa atgtttcttc cagcgacgtc aattattact
     5041 ggggtgctaa ttaccgggct ggaagctcca acgagtttgt cctctaaagc agaaccgatg
     5101 ccggttacca tgcgcattaa aaaagacatc atcatgccta acaattacac gatggatttg
     5161 cgtgattgta atttattggg ttctgctgtg ggggatctgg catctcagcg tgcgtacatt
     5221 agggcaacct ccatatcgtg tgtcaactcg aaaggaaaag cattcgacgt aatagtggaa
     5281 gcttatgcgg ttagtgaaaa tgacgggaaa aatggcattc gtgggaatct gataagcaga
     5341 aatggaaatg cgattgcagg ttctgcgttt gcaggagggc tgtcagcatt ggcgggtagt
     5401 ctgagtccat caaaggtgtc ttcgcttaat atagatccaa actctacggc acaataccaa
     5461 tcgccaaaca taggtgcatt aggagcgctg gccggagccg gtgcagctca ggggggtcta
     5521 aaccgtcttg tggactacta tacgtcaata gctgagcagc agtggccaat tgtagaaatt
     5581 agcccagggc gcccaattac ttttgtcgtt caaaaagggg cgacaatacc tacaaacctg
     5641 acaagccgct gagaataatt atggaaaata acaacgacaa tgaatcacca aaaaagagtg
     5701 agtctgctga tagcttaaat atcgacgcgg ctaaaccggt tgggggtata cgtactacca
     5761 aaaaagtaac tttagagatc gatatcgaga aaactcttaa gtatctgatg gtcgcaggcc
     5821 tggcagttct tatcgtgatc tatggataca agggtggacg ttttgtatat gacaacttca
     5881 aagcaatgtc gcaaccggcc taccagattg ccgtgctgga tatgcaaact ttaagaaaag
     5941 tatttaataa gcaaaatccg gtgttggatt cgcagcaatc caaaactaac tttgaaaatt
     6001 attttaaggc attaatgaag gtttaccgtg aacgtggcta cttggttatt gatgcatcct
     6061 tggcagtaac tattccagag aatgttgaaa tagtctctta tatcgacctt ggtggaagtt
     6121 ttggtgaggt acaatctctt ccaggcgaac ctaaagaaag tgagattcga taattgaaaa
     6181 agttactttt gtctctgctg atttccatta cctcagcgtg cccgccgctt actctggctg
     6241 ttgaaaatgt attaaacaat cgtgatgccg ctgcgttaat ggaaaaggca aaagaaggtg
     6301 gagtatcaac gaaaccttta gacggtgtat tggcgaaaat gaaaagctat aagcctaaag
     6361 atgctattta catccctact ggcggtcttt atttgttcca ggatgaacgt tctcagttga
     6421 tggcggtgac cactgacgga cggtatacaa tcacgggggg gagcgtcatg gacattcttc
     6481 agagaaagtc agtgttatca gtagaggata tacgaaagtc gttctttatt aatttagacg
     6541 acgccccttt ccctcttgaa actgtggcat cgataccttt aggaaaccca aaactcaaac
     6601 gtcaagcggc aatatttatt acgcttgatt gtgacggttg tcaggattta ataaagaaat
     6661 tctatgatga acgcgaaaag tatcgggtcg atatagtttt aatcccctcc cccggcgagc
     6721 caaagcagga attgcgtcaa ttatggtgca gtaaagaaaa aggcaaagtg aatgatcttg
     6781 atatcttacg ttggcttatg ggtaataagg ccgatataga aaaacgactc ctgacgaaag
     6841 aagaggcaga agcatgtccg gctgaaccgt tagtcgcgtc attaatgctg gctggcattt
     6901 ataaattaca gggagtcccc tcagttgtaa gacaggatgg actcgctggt aatggcatac
     6961 cgaaagattt tgattactgg ttaaaacaaa gtgtcgaacc cttactcaaa aacccatttg
     7021 atactaatta aggtaatgaa atgaacatta aaaaagttat tttcagcgct ctggttgttg
     7081 gttcatcttc atacctggcg ggttgtacta ttggcagcgc agaatctgaa tgcccgggaa
     7141 ttgaaaaagg agttatttgt aaaggcccgc gtgaagttat ggaactgacg aacaatcgtg
     7201 atgatctctc cggccttggg gaggaagagg ctaattcggg aaaggggaaa tcggctgtca
     7261 atgacagcca gtaccctgcc caactaacgc cgcctggcgc agtgcagtac cccaagtcgt
     7321 ctgtacttgt taataagcca gtgacataca caacgacgga agtaaaaccg gttggacagc
     7381 tcccggtgat gtatgatcag acattaaaaa tgggggcgcc aacttcaaca attggaccac
     7441 ggccaatcag cgggaatcct gttaattcta atgtacggat gacaacgagt tacaactcaa
     7501 atgggtcatc cgttaacccc tttttgcatc cgtctgctga agtcgtgaaa aaaacaactc
     7561 aggttgttac tcccgcgcct gcaccgcgct atgtagcacc aaattctgat atcaatgcaa
     7621 gtaaagacct ttattccatt aacaatggac agccagtaaa ccctacgctc agctctgggc
     7681 aggtacagca ataccgtaca caaggctaca aacaggcagt cgttgcacca gagcctcttg
     7741 ctgtgcttca gcaaggccgc gtcatgagaa tcacctttgc accatatacg gatgataatg
     7801 acgcgctaaa tctgcctggc tttgtttatg tgaatgtcaa accgcaaact tggattgcgg
     7861 gcaaaaattc aacatcaaat ccggcacgaa ttgttccttt ggaagtccag gatgcggctc
     7921 gtgaaaatat gcaacagcaa cagcgagcaa cgaaagcagt ttcgtcaaac ggtattgtga
     7981 gacaactctg aggtcacgaa tgagatcagc taacgtctat aataaagaat taagccgtca
     8041 tcagttaggt ggatttattc ccgtgtatga tcagattccg ggtacacatt attttctact
     8101 tgacggaaac cgactcggtt ttatgtttat ctgtagtccg tcgcccggtg tctttgataa
     8161 tcagcaggat gtgttaactg aattatttaa aatggacttc ccaactgaca ccatctgtca
     8221 gacctcatta actgcgctgc cagacttgat cttgcactta agtgcctggt ctgccgttcg
     8281 tggtggtcga atggaaggtc atgataagct taagggggat ttactcactg catatcaact
     8341 ggattattat gaccgaagcc tgaatgaacc tttgaagccg gaccacgata aattgatgct
     8401 tcgagatttt caggtctgga tttccttttc aattccattg aagtcagcac ttccttctga
     8461 aatagaaaag actcgcattg atgcgcttta ctctgactta atcagtaagt tgaatacagt
     8521 aggccttttc ccacacaaag ttggtgctga aaattggctt tattgcatgg ataagttatt
     8581 gcatccggga aaaacatcgc gttggtctga aggtcatgtt gaagctagca ccatgagacg
     8641 cctaaacgag cagatcaatg taccgggtcg aaaatataca gtgacagaga atcatttctc
     8701 atctacaacg cagagcaatg atatttctga gcatcgttat ttcaaacaat tatcagtagt
     8761 gaagtttcca gaatttgtaa actttggctg catgtatgaa cttgtagtta attggttgaa
     8821 tggacgtaaa acgatcttta gccctttcat gataacccag actgtccatt tcgccgatcc
     8881 tttaaaatta tcaagagaaa acgtcagata taaggcaatt actaacaaac aagccagtat
     8941 tccaacagtt ttgacgtttt gtccaagatt aaaggatatg gataatgatt acatgacgat
     9001 aacacgcgaa ctggaagatg gggctcgcct cctacacagt tatctgacat ttactgtaat
     9061 ggggaattca gcagttgatg ttcagtctgc cgcagaccag ttaaaatcat tttatctgga
     9121 aagtcgtgtt aatgttgctg atgactcgta catagtattt ccctcatttg tttcaagcct
     9181 gccgatgtgt aatgacccaa agacaatact agaactggat cgttttgagg tcgtgagtaa
     9241 tactggtgct gcgcacatga cccccatatt tggtccttgg aaaggtaata cggacagacc
     9301 cgtacttaac cttgtctcac gagaaggaca gctgttgggg ctggatatct tcaagacttc
     9361 tgccagctat aacatggtcg ttggtgcaac gtctggtgct ggtaaatcat tctgggtcgc
     9421 atacatcatt aataattatc taggcgcagg tccgcgttct aataatctga tccattatcg
     9481 agatactttt gagggattca aaaacaattc ttatgatgca tttgaccctg atggagccca
     9541 gattttcgta gttgatgtcg gacgctcata ccaaggtatt tcggaacaat atactaatag
     9601 tcagtttata gactttggga aaaagcctga ttttacgcta aatccattcg catttttgac
     9661 tgatgtgact gttggagagc gtgtttttga tgaagcacct gtctttaatg acgattcaaa
     9721 taatcatgat gatgataagg ataaagtagc ccagaccatt atggttctaa accagttaaa
     9781 aataatggca tctgaaaaag gaaatattga cgattttcaa caatcggtca tgctgcaact
     9841 gattagtgaa gaatataacg aatcacgcaa agttggacgt acaggctcta ttactggatt
     9901 tgcgcgacgc tgttctaacc atgaagataa gcgtattaaa gatatcggtg accagttggg
     9961 gcaatggtgt gaaggaggga tttatgggaa tcgctttaca gaaaatctcc cgccaataaa
    10021 ttttgatagc cggtttattg ttctggaact tgaagaattg aaggggaccc cacaccttca
    10081 gactgtcgtt ctgatgtcga ttattcaggc cgctcagcat gcaatgttta tcaagaaaga
    10141 cggacgccgc cggttgttca tacttgatga ggcatgggag tatatccgtc cggacaatgc
    10201 ttcaggttcc agtaatcatt caaatcaatt tttctcctct ttccttgagg ccgcttggcg
    10261 ccgattcagg aaaacgaact gtgccggtat ttgtattact caatcctttg aagattactt
    10321 cacctcatcg gttggtcgcg cactaacggc taactcgccg tggaagatca tcatgaagca
    10381 ggaaaaggaa agtattgaag ccatgaaggt gaacaactac ttctccacaa ctgatgcgga
    10441 atatgagcgt atgaaaaata ttcgaacggt aaagcgtgca ttttccgaga tgctcgtacg
    10501 attcgaaaat ttccaggaga tatgtcgcct ctatgtagat agaaaaatgg agctttgctt
    10561 tacgactgac agcaccgatc gtggaaaact atgggaaatc caaagccgac ttgattgctc
    10621 ttacggcgaa gcaattgaga ttctgtacga acaggaagtt gcgagcaaat cagctgcctg
    10681 acgttttaag ttactggtta cctcattttg ttttgactga ggtatccagt acgtctttga
    10741 agtaaatagt tgttagctgt cattgcctgc cctcccccga aataatagcc cgggttaaga
    10801 atatctagct cggattaagt cctctcaaac cgtcatacat gaaattcgat gcctttgtca
    10861 ttcttctttt gaaggtgaat gaatatgatg aaatttactc cagaattatt ctccctacca
    10921 ggcttttgct gtgaagttaa tcagatcagg tattatgttt tgggtacaag aaccaaccaa
    10981 ggggggtgtg gtttttgtat g
//
LOCUS       DAPHBJ010000145        10951 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.147, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000145 DAPHBJ010000000
VERSION     DAPHBJ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.147"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            11..634
                     /gene="nfeR"
                     /locus_tag="RHZ76_003356"
                     /gene_synonym="yqjI"
     CDS             11..634
                     /gene="nfeR"
                     /locus_tag="RHZ76_003356"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HDV2813946.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(788..2308)
                     /gene="aer"
                     /locus_tag="RHZ76_003357"
     CDS             complement(788..2308)
                     /gene="aer"
                     /locus_tag="RHZ76_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HDV2813947.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVESLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVAHQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            2726..4105
                     /gene="ygjG"
                     /locus_tag="RHZ76_003358"
     CDS             2726..4105
                     /gene="ygjG"
                     /locus_tag="RHZ76_003358"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HDV2813948.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(4147..4479)
                     /locus_tag="RHZ76_003359"
     CDS             complement(4147..4479)
                     /locus_tag="RHZ76_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDV2813949.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            4698..5681
                     /gene="ebgR"
                     /locus_tag="RHZ76_003360"
     CDS             4698..5681
                     /gene="ebgR"
                     /locus_tag="RHZ76_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HDV2813950.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFVEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLSREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            5865..8957
                     /gene="ebgA"
                     /locus_tag="RHZ76_003361"
     CDS             5865..8957
                     /gene="ebgA"
                     /locus_tag="RHZ76_003361"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HDV2813951.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSTIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYPQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            8954..9403
                     /locus_tag="RHZ76_003362"
     CDS             8954..9403
                     /locus_tag="RHZ76_003362"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HDV2813952.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            9466..10899
                     /locus_tag="RHZ76_003363"
     CDS             9466..10899
                     /locus_tag="RHZ76_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV2813953.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
BASE COUNT         2613 a         2949 c         2777 g         2612 t
ORIGIN      
        1 ggaggctgat atgagccatc atcacgaagg gtgttgtaaa catgaaggcc agccacgcca
       61 tgagggctgc tgcaaaggtg agaagtcaga acacgagcac tgcggacacg gtcaccagca
      121 tgaacacggt caatgctgcg gtggtcgcca cggtcgcggc ggcggtcgtc ggcaacgttt
      181 ctttggtcac ggtgaattgc gtctggtgat tctggatatt ctctcgcgcg atgacagcca
      241 cggttacgaa ttgattaaag cgattgagaa tctaacccag gggaattaca ccccaagccc
      301 gggcgtcatc tacccaacgc tggattttct gcaggagcag tcgctgatta ccatccgcga
      361 agaggaagga ggtaagaagc agattgcgct gaccgaacaa ggcgcgcagt ggctggaaga
      421 aaaccgcgaa caggtggaga tgattgaaga acgcatcaaa gcgcgttgcg ttggcgcggc
      481 gctgcgccag aacccgcaaa tgaagcgggc gctggataat tttaaagcgg tgctggattt
      541 acgcgtcaac cagagcgata tcagtgatgc acaaataaaa aagatcattg cggtgatcga
      601 ccgcgccgct tttgatatta cgcaactgga ttaatcgccg catccgccag tggcgcggtg
      661 caattgccgg atgcgacgct tgacgcgcct tatccggcct acacccgcta cacaccccgc
      721 aggcctgata agatgcgcca gcatcgcatc aggcattgtg ctccaaccgc cggatccggc
      781 ataccgatta atgcagtacc gtcaccgcgt cttccagtcg gctggcgcgg tgtttcacca
      841 tcgccgacac ctgcgcactc tcttccacca gctcggcatt tttctgggtg atcaggttaa
      901 gctcatccac tgcacgggtc aggctggaaa gcccatcggc ctgttccagc gttgaatggc
      961 taatctgggc gatcaactgg gtgacgtttt tcacctgtgc cacaatatct tccatcgtcc
     1021 gtccggcggc gtgtacctgc tgcgaaccgg attgcacctt atcagcactg gcatcaatca
     1081 gcttgcgaat atcgttggca gcattagcgc tgcggctggc taaatgacgc acttcccctg
     1141 ccaccacggc aaaacctttg ccctgttcgc cggcacgcgc cgcttccacc gccgcattca
     1201 gggccagaat attggtctga aacgcaatat cgttaatcag cgaagtaatg gtgccaatgc
     1261 gctgggtact gtcggcgata tcgtccattg tcttgatcac cgtggtcatc gcctccccac
     1321 cctgcactgc cgcattactg gcagtgattg acagtttatc ggcagccgac gccgtggcag
     1381 agttctgttt caccgacgcc gccatttggt tcatggtcgc caccgtttgc tgaacgttat
     1441 caactgtctg ctgggtatgt tcgttcagtt catcggtgcc tttcgccagc gtctcactgc
     1501 catttctgac actggacacc tggcttgaga catcgttaat tagccaacgg cacatcaggc
     1561 caagttgccc taccgcacgt aatgtcagcc ccagctcatc gctgcgattc agatgctcaa
     1621 cactattacg ttctccggtc gccaccttca gtgcctgatg ggcaacattt tctatcgggc
     1681 gcacaatctg ccattcaaaa caggcgcttg ccaacaatac cactaacgca cagaggatat
     1741 acgtcaccac cggggcagca acaaaccaaa gcatggccgc cagcaagata aacatcaggg
     1801 tcatcactcc acgcgcccgc cagcgaagcg gtaatgaagg cagtttaccc agccagcctt
     1861 tacgcaccac caggccttta tgaatacgct tactggtacg tccggcgttc aacgctttgt
     1921 acagcgactc caccgccgcg atctcttcat ccgtcgcccg ggtacgaatc gacatatagc
     1981 cactgatttt tccctcgcgc accatcggta ccgcattggc ccgcacccaa taatggtcac
     2041 catttttgcg gcgatttttc acgatgccgc tccagggctc cccttttttc agggtgaacc
     2101 acatatccgc aaacgccgct tttggcatat ccgggtgacg caccatgttg tgcggctgcc
     2161 cttgtaactc ttgcaaggta tagccgctca cctgcacaaa agtgtcatta gcatgagtga
     2221 tatagctttg cagatcggta gtggacatca gagtggtatc gtccgccagc ggggtatttt
     2281 gctgggtgac atacggatga gaagacatgt tcgcgtcctg tgcaggttat atggttgtta
     2341 acttcttgtc agagtttatg tcggccccgc tgcggttatc tttaaccgat taatttgatt
     2401 tagatcgcaa tttgcgattt aaacacaaat ctaattcctt gatttaaaat actttcactc
     2461 tgttactata cgaaaacgtt aattatcttg cccaaaaatc aggcaattat tgccctgaaa
     2521 acgtgcattt gcgcagcaat catcaaatcc atacccgaca aaaaccgtgc aaaataacaa
     2581 caaatgttaa cagatagcat taaatattgc acaaatgata accgaatttg tgtttatccc
     2641 gattttcgcg atcgcagccg gagtggcgca atccctgcaa tacttaaatc ggtatcatgt
     2701 gatacgcgag cctccggagc atattttgaa caggttacct tcgagcgcat cggctttagc
     2761 gtgcagcgcc cacgccctga atctcattga gaagcgaacg ctggatcatg aggagatgaa
     2821 agcacttaac cgagaggtga ttgaatactt caaagagcat gtcaatccgg ggtttttaga
     2881 gtatcgcaaa tctgttaccg ccggcgggga ttacggagcc gtagagtggc aagcgggaag
     2941 tttaaatacg cttgtcgaca cccagggcca ggagtttatc gactgcctgg gaggttttgg
     3001 aattttcaac gtggggcacc gtaatccagt tgtggtttcc gccgtacaga atcaacttgc
     3061 gaaacaaccg ctgcacagcc aggagctgct cgatccgtta cgggcgatgt tggcgaaaac
     3121 ccttgctgcg ctaacgcccg gtaaactgaa atacagcttc ttctgtaata gcggcaccga
     3181 gtccgttgaa gcagcgctga agctggcgaa agcttaccag tcaccgcgcg gcaagtttac
     3241 ttttattgcc accagcggcg cgttccacgg taaatcactt ggcgcgctgt cggccacggc
     3301 gaaatcgacc ttccgcaaac cgtttatgcc gttactgccg ggcttccgtc atgtgccgtt
     3361 tggcaatatc gaagccatgc gcacggctct taacgagtgc aaaaaaaccg gtgatgatgt
     3421 ggctgcggtg atcctcgaac cgattcaggg tgaaggtggc gtaattctgc cgccgccggg
     3481 ctatctcacc gccgtacgta agctatgcga tgagttcggc gcactgatga tcctcgatga
     3541 agtacaaacg ggcatggggc gcacgggcaa gatgttcgcc tgcgagcatg agaacgtaca
     3601 gccggatatc ctctgccttg ccaaagcgct cggcggcggc gtgatgccga ttggcgcgac
     3661 catcgccact gaagaggtgt tttcagttct gttcgacaac ccattcctgc ataccaccac
     3721 ctttggcggc aacccgctgg cctgtgcggc ggcgctggcg accatcaatg tgttgctgga
     3781 gcagaactta ccggctcagg ctgagcaaaa aggcgatatg ttgctggacg gtttccgtca
     3841 actggcgcgg gaatatcccg atctggtaca ggaagcgcgt ggtaaaggga tgttgatggc
     3901 gattgagttt gttgataacg aaatcggcta taactttgcc agcgagatgt tccgccagcg
     3961 cgtactggtg gccggaacgc tcaataacgc caaaacgatc cgcattgaac cgccactgac
     4021 actgaccatt gaacagtgtg aactggtgat caaagcggcg cgtaaggcgc tggcggccat
     4081 gcgagtaagt gtcgaagaag cgtaatatca aatcggatgg cgatacgacg tcgccatccg
     4141 atctttttat acgacgcgta cgcccgcagg catcatccgc tccggcgtta acagcacgct
     4201 ttcactgccg tcatcggttt ccgcacacaa cagcatgcat tccgacgttt caccacgcat
     4261 tttcgctttt tgcagattgc ataagaccac caccgttttt cccatcaact cttcttcgct
     4321 gtagtacggc accaggctgg tcacggtttg cagtgttttt tcccccacat caacctgtac
     4381 gatgtacagc ttgtcggcgt tttcatggcg tttcacttcc acaatctttc cgacgcgcat
     4441 ttccagacgt gcaaaatcag cgtaagccac ggtttccatt gcttcctccc ttcgagtaaa
     4501 attttactaa actatagcaa aaatttttct caatcctgta ggctaaaaat ggagaatgca
     4561 ggcgtgatca cattcgtgag ccgctgtgtt accgttacag cgtcaaagaa acgcgcttta
     4621 tttactgaaa acaggtgacc caataagcac ttcctctaca atggaagcgc acatcaggga
     4681 aagtaaaaaa ggtaaacatg gcaacactaa aagacatcgc aatcgaagct ggcgtatccc
     4741 tggcgacagt atccagggtc ttaaatgacg atccgacatt gaatgtgaaa gaagagacga
     4801 aacatcgcat tctcgagatc gccgaaaagc tggagtacaa gaccagtagc gcccgtaaac
     4861 tacagacagg cgcagtcaac caacaccata ttctggctat ctacagctac cagcaggagc
     4921 tggagatcaa cgatccttac tatctggcga tccgccacgg cattgaaacc cagtgcgaaa
     4981 agctgggcat cgagctcacc aactgttatg aacacagcgg attaccagac attaaaaacg
     5041 tcaccggtat tttaattgtc ggcaaaccca cgcccgccct gcgcgccgct gccagcgcgt
     5101 tgaccgacaa tatctgtttt atcgactttc acgaacccgg cagcggttac gatgcggtgg
     5161 atatcgatct ggcacgcatc agtaaagaaa tcatcgactt ctatatcaac cagggcgtta
     5221 atcgcattgg ttttattggc ggtgaagatg agcctggcaa ggcggatatt cgtgaggtcg
     5281 cctttgtgga atatggccga ctgaaacaag tggtacgcga agaggatatc tggcgcggcg
     5341 gtttttccag ttcgtcgggc tatgaactgg caaaacagat gctgtcgcgg gaagactatc
     5401 cgaaggcact gtttgttgct tccgattcca ttgctatcgg cgtactgcgg gcaattcatg
     5461 aacgtggcct gaacatccca caggatattt cgcttatcag cgttaacgat atccccaccg
     5521 cgcgatttac ctttccgccg ctctccaccg tgcgcatcca ttccgagatg atgggaagtc
     5581 agggcgttaa cctggtgtat gaaaaagccc gcgatggtcg cgcgctgccg ctgttagtct
     5641 tcgttcccag caaattaaaa ctgcgcggca cgacccgtta aatcccctta cacactgtcc
     5701 ggcagtcgtt tttgccggac agtgctgccg tttattttcg tgatccagtt aaagtaaatg
     5761 catttaactg ctacttttta gtaaaaattt tactaaactc cccagcaatt acacaaacta
     5821 ccatcaccat gaatggttcc gatttctctc taccgggagg ccctatgaat cgctgggaaa
     5881 acattcagct cacccacgaa aaccgacttg cgccgcgtgc gtactttttt tcatatgatt
     5941 ctgttgcaca ggcgcgtacc tttgcccgcg aaaccagcag cctgtttctg cccttaagcg
     6001 gtcagtggaa tttccatttt tttgaccacc cgctgcaagt gcctgaagcc ttcacctctg
     6061 agttaatggc tgactggggg catattaccg tccccgccat gtggcaaatg gaaggtcacg
     6121 gcaaactgca atataccgac gaaggttttc cgttccccat cgatgtgccg tttgtcccca
     6181 gcgataaccc aaccggtgcc tatcaacgta ttttcaccct cagcgacggc tggcagggta
     6241 aacagacgct gattaaattt gacggcgtcg aaacctattt tgaagtctat gttaacggtc
     6301 agtatgtggg tttcagcaag ggcagtcgcc tgaccgcaga gtttgacatc agcgcgatgg
     6361 ttaaaaccgg cgacaacctg ttgtgtgtgc gcgtgatgca gtgggcggac tctacctacg
     6421 tggaagacca ggatatgtgg tggtcggcgg ggatcttccg cgatgtttat ctggtcggaa
     6481 aacacctaac gcatattaac gatttcactg tgcgtaccga ctttgacgaa gcctattgcg
     6541 atgccacgct ttcctgcgaa gtggtgctgg aaaatctcgc agcctcccct gtcgtaacga
     6601 cgctggaata taccctgttt gatggcgaac gcgtggtgca cagcagcacc attgatcatt
     6661 tggcaattga aaaactgacc agcgccagct ttgcttttac tgtcgaacag ccccagcaat
     6721 ggtcagcaga atccccttat ctttaccatc tggtcatgac gctgaaagac gccgacggca
     6781 acgttctgga agtggtacca caacgcgttg gcttccgtga tatcaaagtg cgcgacggtc
     6841 tgttctggat caataaccgt tatgtgatgc tgcacggcgt caaccgtcac gacaacgatc
     6901 atcgcaaagg ccgcgccgtt ggaatggatc gcgtcgagaa agatctccag ttgatgaagc
     6961 agcacaatat caactccgtg cgtaccgctc actacccgaa cgatccgcgt ttttacgaac
     7021 tgtgtgatat ctacggcttg tttgtgatgg cggaaaccga cgtcgaatcg cacggctttg
     7081 ctaatgtcgg cgatatcagc cgtattaccg acgatccgca gtgggaaaaa gtctacgtcg
     7141 agcgcattgt tcgccatatt cacgcgcaga aaaaccatcc gtcgatcatc atctggtcgc
     7201 tgggcaatga atccggctat ggctgtaaca tccgcgcgat gtaccatgcg gctaaagcgc
     7261 tggatgacac gcgactggtg cattacgaag aagatcgcga tgctgaagtg gtcgatatta
     7321 tttccaccat gtacacccgc gtgccgctga tgaatgagtt tggtgaatac ccgcatccga
     7381 agccgcgcat catctgtgaa tatgctcatg cgatggggaa cggaccgggc gggctgacgg
     7441 agtaccagaa cgtcttctat aagcacgatt gtattcaggg acattatgtc tgggaatggt
     7501 gcgaccacgg gatccaggcg caggatgaca acggcaatgt ctggtataaa ttcggcggcg
     7561 actacggcga ctatcccaac aactataact tctgtcttga tggtttgatc tattccgatc
     7621 agacgccggg accaggcctg aaagagtaca aacaggttat cgcgccggta aaaatccacg
     7681 cgctggatct gactcgcggc gagctgaaag tcgaaaataa actgtggttt accacgcttg
     7741 atgactacac cctgcacgca gaggtgcgcg ccgaaggtga aacgctcgca acgcagcaga
     7801 ttaaactgcg cgacgttgcg ccgaacagcg aagccccctt gcagatcacg ctgccgcagc
     7861 tggacgcccg cgaagcgttc ctcaacatta cggtgaccaa agattcccgc acccgctaca
     7921 gcgaagccgg gcattctatc gccacttatc agttcccgct gaaggaaaac accgcgcagc
     7981 cagtgccttt cgcaccaaat aatgcgcgtc cgctgacgct ggaagacgat cgtttgagct
     8041 gcaccgttcg cggctacaac ttcgcgatca ccttctcaaa aatgagtggc aaaccgacat
     8101 cctggcaggt aaatggcgag tcgctgctga ctcgcgagcc aaagatcaac ttcttcaagc
     8161 cgatgatcga caaccacaag caggagtacg aagggctgtg gcagccgaat catttgcaga
     8221 tcatgcagga acatctgcgc gactttgccg tagaacagag cgatggtgaa gtgttgatca
     8281 tcagccgcac ggttatagca ccgccggtgt ttgacttcgg gatgcgctgc acctacatct
     8341 ggcgcatcgc tgccgatggc caggttaacg tggcgctttc cggcgagcgt tacggcgact
     8401 atccgcacat cattccgtgc atcggtttca ccatggggat taacggcgaa tacgatcagg
     8461 tggcgtatta cggtcgtgga ccgggcgaaa actacgccga cagccagcag gctaacatca
     8521 tcgatatctg gcgcagcacc gtcgatgcca tgttcgagaa ctatcccttc ccgcagaaca
     8581 acggcaaccg tcagcatgtc cgctggacgg cactgactaa ccgccacggc aacggtctgc
     8641 tggtggttcc gcagcgccca attaacttca gcgcctggca ctatccccag gaaaacatcc
     8701 acgctgccca gcactgtaac gagctgcagc gcagtgatga catcaccctg aatctcgacc
     8761 accagctgct tggcctcggc tccaactcct ggggcagcga ggtgctggac tcctggcgcg
     8821 tctggttccg tgacttcagc tacggcttta cgttgctgcc ggtttctggc ggagaagcta
     8881 ccgcgcaaag cctggcgtcg tatgagttcg gcgcagggtt cttttccaca aatttgcaca
     8941 gcgagaataa gcaatgagga tcatcgataa cttagaacag ttccgccaga tttacgcctc
     9001 tggtaagaag tggcaacgct gcgttgaagc gattgaaaat atcgacaaca ttcagcctgg
     9061 cgtcgcccac tccatcggtg actcattgac ttaccgcgtg gagacagact ccgcgaccga
     9121 tgcgctattt accgggcatc gacgctattt tgaagtgcat tactacctgc aagggcagca
     9181 aaaaattgaa tatgcgccga aagagacatt acaggtagtg gaatattatc gtgacgaaac
     9241 tgaccgcgaa tatttaaaag gctgcggaga aaccgtagag gtccacgaag ggcaaatcgt
     9301 tatttgcgat atccatgaag catatcggtt tatctgcaat aacgcggtca aaaaagtggt
     9361 tctcaaagtc accatcgaag atggttattt ccataacaaa taacaactac ggcggcaaaa
     9421 ggagtttgcc gccaccgcta ccctacacat tttcggagat gtgttatgtc tgataccaaa
     9481 cgtaatacaa tcggcaaatt cggcttgctc tcgctgactt ttgccgccgt ttacagcttt
     9541 aacaacgtta tcaataataa tattgagctt ggactggcct cggcaccgat gtttttcctc
     9601 gcgacgattt tttattttat tcccttctgt ctgatcatcg cagaatttgt ttcgttaaat
     9661 aaaaactcag aagccggtgt ctacgcgtgg gtaaaaagtt cgctgggcgg gcgttgggca
     9721 tttattactg cctataccta ctggttcgta aacctgttct ttttcacctc gctgttgccg
     9781 cgcgttattg cttatgcttc gtatgccttc ctcggctacg aatatattat gacgccggtt
     9841 gccaccacca ttatcagtat ggtgctgttc gccttctcca cctgggtttc caccaacggg
     9901 gcgaaaatgt tggggccaat tacctccgtc acttcaacgc tgatgctgct gttaacgctc
     9961 tcctacattt tactggcagg tacggcgctg gttggcggcg tacagcctgc tgaccccatc
    10021 accgttgacg cgatgatccc gaacttcaac tgggcgttcc tcggcgttac cacctggatc
    10081 tttatggccg caggtggcgc ggagtccgtc gcggtgtacg ttaacgacgt caaaggcggt
    10141 tcgaaatcgt tcgttaaagt gatcatcctc gccgggattt ttatcggcgt actgtattcc
    10201 gtctcctcgg tgctgattaa cgtcttcgtc agcagcaaag agttgaaatt taccggcgga
    10261 tcggtgcagg tattccacgg catggcggcg tattttggtc taccggaagc gttgatgaat
    10321 cgctttgtcg gtctggtgtc ctttaccgcg atgttcggtt ccctgctgat gtggaccgca
    10381 acgccggtga aaattttctt ctccgaaatc ccggaaggca tctttggtaa gaaaaccgtc
    10441 gaactgaacg aaaacggcgt tccggcgcgc gcagcgtgga tccagttcct gatcgtcatc
    10501 ccgctgatga ttatcccgat gctcggttcc aataccgtgc aggatctgat gaatactatt
    10561 attaatatga ccgccgcagc gtccatgctt ccgccgttat tcatcatgct ggcttacctg
    10621 aatttacgcg ccaaattaga tcacctgcca cgcgatttcc gtatgggctc ccgccgcacc
    10681 ggtattatcg ttgtttcaat gctgattgcg atatttgccg tagggtttgt cgcttcgaca
    10741 ttcccgactg gcgcgaatat tctgaccatc attttttata acgtcggcgg tattgttatc
    10801 ttccttggct ttgcgtggtg gaaatacagt aaatatataa agggattaac ggctgaagag
    10861 cgccatattg aagcgacgcc agccagcaat gttgattaag cagaataata aaaaatgagc
    10921 aggcattttc cctcttcctg gatgaggaga g
//
LOCUS       DAPHBJ010000146        10929 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.148, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000146 DAPHBJ010000000
VERSION     DAPHBJ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.148"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(54..773)
                     /gene="dcuR"
                     /locus_tag="RHZ76_003364"
     CDS             complement(54..773)
                     /gene="dcuR"
                     /locus_tag="RHZ76_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HDV2813954.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(770..2401)
                     /gene="dcuS"
                     /locus_tag="RHZ76_003365"
     CDS             complement(770..2401)
                     /gene="dcuS"
                     /locus_tag="RHZ76_003365"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HDV2813955.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2582..2812
                     /locus_tag="RHZ76_003366"
     CDS             2582..2812
                     /locus_tag="RHZ76_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDV2813956.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            2824..3096
                     /locus_tag="RHZ76_003367"
     CDS             2824..3096
                     /locus_tag="RHZ76_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV2813957.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            3323..3619
                     /gene="ghoS"
                     /locus_tag="RHZ76_003368"
     CDS             3323..3619
                     /gene="ghoS"
                     /locus_tag="RHZ76_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDV2813958.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            3647..3820
                     /gene="ghoT"
                     /locus_tag="RHZ76_003369"
     CDS             3647..3820
                     /gene="ghoT"
                     /locus_tag="RHZ76_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HDV2813959.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(3939..5456)
                     /gene="lysS"
                     /locus_tag="RHZ76_003370"
     CDS             complement(3939..5456)
                     /gene="lysS"
                     /locus_tag="RHZ76_003370"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDV2813960.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(5693..7150)
                     /gene="dtpC"
                     /locus_tag="RHZ76_003371"
     CDS             complement(5693..7150)
                     /gene="dtpC"
                     /locus_tag="RHZ76_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HDV2813961.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(7209..9356)
                     /gene="cadA"
                     /locus_tag="RHZ76_003372"
     CDS             complement(7209..9356)
                     /gene="cadA"
                     /locus_tag="RHZ76_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HDV2813962.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(9436..10770)
                     /gene="cadB"
                     /locus_tag="RHZ76_003373"
     CDS             complement(9436..10770)
                     /gene="cadB"
                     /locus_tag="RHZ76_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HDV2813963.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
BASE COUNT         2971 a         2588 c         2589 g         2781 t
ORIGIN      
        1 ggggtatttt gcattacagt aataattatt acttacagat aaccagcgat ccgttattgg
       61 caatattgtt tcagtagtga gtagtgttct gcctgaatac ggtaacggta aactggacgc
      121 cccgtgacgc cataatggat actggtgaac aagatgtggc agttgaccag ccagatgagg
      181 tatttacggc aggaaacacg cgaaatgtta acctcgttgg ctagctcgtc ggttgaaaac
      241 tcatagtcct gatgcgcgtc aatccactgg cacagcgtgc gtaacgtctg cggcgttaag
      301 ccttttggca agcgacgagg atcctgttcg ttggagctgc tgccgtggat tagctgatca
      361 agctcggcct ggtcataata ctgatgtttt tccagcgcca ttttcttttg ccgccagccg
      421 gtgagcgcct cttcaaagcg ggaagcctgg aagggtttga tcaggtaatc cacgacaccg
      481 taatgcagcg aatctttaat ggttgccgca tcggctgcgg aggagatgac aatcacatca
      541 cttttgcaac gcgcgttatg caggacaggc agtaaatcga gcccgttctc tttttgcata
      601 tagatatcga gcaatatcag gtcgataggc gtatcgctat tgaagataat ctctttggct
      661 ttctccagcg tcgaggctgt tccacagcat tgaaagcctg ggatttgtgc tacgtatcgg
      721 cgattcagct ccgcgaccat tgcgtcgtca tcgataatta atacattgat catctgttcg
      781 acctctcccc gtcccagggt atctggacaa aaaattgtgt gaaaatcccg ggttccgatt
      841 ccacggcgat gctgccgccg agattttcta cctgttgttt gacaagtgct aaaccgacgc
      901 ctcgctcgct tccttttgtc gagacacctt tgtcaaaaat gtgatcgatt ttgtcgggtg
      961 cgatccccgg tccatcatcg ttaacttcgc agtgcagcca gccgtgacgg tagtgcaatg
     1021 ttacgctaat ttcgccaccg ggttccggcc ctaatgcttc cagcgcgttt tctatcagat
     1081 ttcccaatgt ggtaatcagc gtcgcgacct ggtcctcact gccgctgtct ggcagctggc
     1141 tttcactgtt taaaatcagc gtatggccta aatcggtcgc gcggttaatc ttgctgatta
     1201 aaaaaccagc gataaccgga gatttgatct tacccagcag agagccaatc tcttcctgat
     1261 agttattggc tgttttgaga atgtaatctt ccaactgctt ataactcttc agatgcaata
     1321 atccgagaat cacatgcaat ttattcataa attcgtggga tcgttcacga agtgcatcag
     1381 catagttgac cagaccgtcg agtcgctgca tcagtttacg aacttcagtt ttgtccctga
     1441 aggttgaaat ggcaccgatg ataacgccat tactgcgcac cggaacggtg ttgatcagta
     1501 atagccggtc tttaatcgta atctcttcgt cgcggcgcgg cgtaccgtcg cgtaacactt
     1561 ccgagacatc tactacctgt gaccacgagt ggcttagcgt cgacagtttc tcatcgtcct
     1621 gcgacttacg gtaattcagc aattcttgtg cggcatcgtt gatcagcgtg acctcgccgc
     1681 gatcgtccac ggcaacgacg ccttctttga tagactgcaa catggcctgg cgttgctcaa
     1741 acagcgtgga gatttcgtag ggttccaggc cgaaaaggat ttttttcagt actttaacca
     1801 gaatgcaggt gccaatcagt ccgaccagca tgccaaatag taccgaccag ataatgctcc
     1861 agcgactgtc attgatttgt tgggtcacgc ggcttaactc aaggccgatc gccaccacgc
     1921 caatttgttt atgattttca tcgtagatgg gggtaaatac gcgtaaagcc tgcgccagaa
     1981 aaccgcgatt gatagcgaca ttttcttcgc cattcagcgc tttaaggatg tcatcacctt
     2041 taaatggctg accaatacgc tgggcttcag gatgtgagta gcgaagactt tgcatatcgg
     2101 taacgacaat aaacagcaga tcgttgcgtt tgcgcacggc ttccgcgatg gcctggattc
     2161 cactctcctg cggttttttc tgcaagccct gacggatttc cggcgagtcg gcgagagtac
     2221 gcgccactgc cagtgccttg ttggccagcc cgtctcgcgt catatcactg atttgcgaga
     2281 agtaaatcag atgcaccacc aatagcaccg agaacagtac cgcgctgacc attaagatca
     2341 ctgtggtact caatttcatc ggacgtttgc gtaacatgcg gtagggcaat gaatgtctca
     2401 tcagcttcct tgtgtgacaa atttcttaag cattatctct gatgaggcgg gtaattcaaa
     2461 gggagtaaga atgattggct atatagggga agagactctg gcaacggaaa ctgccagtgc
     2521 tgtatgaaga ttccggggct atgcttatag tgaaaaccat actgatgaga gagggaaggt
     2581 catggatcag gcgctacttg acgggggtta tcgctgttat accggcgaaa agatcgatgt
     2641 ctatttcaac actgcgatat gtcagcattc tggcaattgc gtacgtggca acggcaagtt
     2701 atttaatctc aaacgaaagc cgtggatcat gccggatgaa gtcgacgttg ccactgtggt
     2761 taaagtgatt gatacgtgcc cgagcggcgc gctgaaatac cgtcataaat aagcgagggt
     2821 aaaatggaaa tacgcgaagg ccacaataaa ttttacatta atgacgaaca aggtaagcaa
     2881 atcgctgaaa ttgtctttgt gccgaccgga gagaatttag cgattatcga acataccgat
     2941 gtcgatgaaa gcctgaaagg gcaagggatt ggtaaacagc tggttgcgaa agtcgtggaa
     3001 aaaatgcgtc gggaaaaacg aaaaattatc ccattatgcc catttgcgaa acacgaattt
     3061 gataaaacgc gggagtatga tgatattcgc agttgatggg agagtacaga gtcacgatat
     3121 ttttcatgct ctccgcggag tgatgcagga gagcatctga agggtagggg gatgcacaaa
     3181 gaatgggcag agagcgcgtt tttttgtccc aagtcatccc ctttactgag caaaaaaaag
     3241 aatatctcct atatgagaat catcaatcgg ggttaataag ttttgcgtcc ccagagcatt
     3301 caatattgat aggagtcata ttatggaagg taaaaacaag ttcaatactt atgttgtttc
     3361 ttttgattat ccatcatctt attcctcagt gttcttaagg ttaagatcat tgatgtatga
     3421 tatgaatttc tcttctatcg tggctgatga atatgggata ccacgacaat tgaatgaaaa
     3481 ttccttcgca ataacgacat cgttagccgc aagtgaaatc gaagatttaa tcaggcttaa
     3541 atgcttagac ttaccggata ttgattttga cctcaacatt atgacagttg atgactattt
     3601 ccgtcagttt tacaagtagc caggaaggat aagaagaaca taaaaaatgg cactattctc
     3661 taaaatatta attttttatg tgattggtgt gaacatatcc tttgtcatta tctggtttat
     3721 ctcacatgag aaaacacata ttcgtttact tagtgcattc ctggtcggaa taacctggcc
     3781 aatgagtctg cctgtggcat tacttttttc tctcttttag gagctgtagt tgagtggacc
     3841 cgctggttat cgtgaaacga agcgtctctc ctcaaaactg gatttgcgga acgacgaacg
     3901 aagcagtcgc ctgactgctt cgttcattaa agtgaaattt atttctgtgg gcgcatcgcc
     3961 gggaagagaa taacgtcgcg aatagtatgg ctgtttgtaa acagcataat cattcggtcg
     4021 ataccaatac ccagaccagc ggttggcggc agaccatatt ccagcgcagt cacgtaatct
     4081 tcgtcataga acatggcttc gtcgtcacct gcagctttag cgttaacctg ttcctggaaa
     4141 cgttcagcct gatcttctgc gtcgtttaat tcgctaaaac cattaccgat ttcacgacca
     4201 ccgatgaaga attcaaaacg gtcggtgatt tccgggttaa catcattacg gcgtgccagc
     4261 ggggacactt ctgccggata ttccgtaata aaggttggct gaatcagatg tgcttctgcc
     4321 acttcatcaa agatctctgt gacaatacgt cccaaccccc agcttttctc taccgtaatt
     4381 ccgatagatt cagctaatgc tttagcagca tcaaaattat ccaggtcggc catatcggtt
     4441 tctggacgat attttttgat tgcttcgcgc atggtgagtt tttcaaacgg tttgccgaaa
     4501 tcaaacacat gctcgccata agtgacttta gtggtaccca gaacctcttg tgccagggtg
     4561 cggaacagtg actctgtcag ttcaatcaaa tcgtggtaat ccgcatacgc catgtagagt
     4621 tccatcattg tgaactcagg attatggcga acagaaatac cttcattacg gaagttacgg
     4681 ttgatttcga atacccgttc aaaaccgcct acaaccagac gtttcagata cagctccggc
     4741 gcgatacgca gatacatatc taaatccaga gcattatgat gggtaataaa cgggcgagca
     4801 gatgccccac ctggaattac ctgcatcatc ggggtttcta cttccataaa gccgcgcgcg
     4861 accatgaatt gacggatagc ggccagaatt tttgaacgga caacaaacgt ttgacgggat
     4921 ttatcgttag cgatgaggtc cagataacgt tgacgataac ggacttcctg atcctgcaga
     4981 ccatggaatt tatctggtaa aggacgtagt gctttagtca gcaggcgcag ctcagtacag
     5041 tgaatggaaa gctcacccgt ttgcgtctta aacagcgtac cgcgggcacc gataatgtca
     5101 cccagatccc attttttaaa ctgatcgtta taaacacctt ctggcaggct atctcgtgca
     5161 acgtacagtt gaatacggcc accgacatcc tgcaacgtta caaaggaggc tttccccatg
     5221 atacgacggg tcatcattcg gccagcaacc gagacttcaa tgtttaagga ttccagttcc
     5281 tggttattct tcgcatcaaa ctcttcgtgc aactggtcag aggtatggtc gcggcgaaaa
     5341 tcattgggaa acgccacacc ttgctgacgt agtgccgcca gtttttcgcg gcgatttctc
     5401 agttcatcgt taaaatcaat agcctcattg gctccccgtg tttcttgttc agacattttg
     5461 gttcctctaa atccagcttt caatttagcg tagataaaga gacagatcgg tacactgtat
     5521 tatctgcctc gactattagt aactcagttt atcttatcga aactaacgct tacctcaagt
     5581 tgatatcact aataaataac catcaaaatg gtttaaatga cttattaatc tcgatttact
     5641 agtgtgataa actgaaagtt aatttattca taaccaattg tttttaggac agttaatcgt
     5701 tgctctcctg tatcatattc gtcggcgtgc taaacagaaa acgggtagca aaggccaata
     5761 cgacgatgat cgcgacacaa gccaacgtcc actctcccat ctgagaaaag aatcgctggt
     5821 aagctgcaat tgccataccg ctaatttgcg actctgtcgt ctgctgtgcc acgacgcctg
     5881 ccagccagtt ggcgaccgcg cctgttgcca gcatataaat accggttaat acgccagaca
     5941 ttttcagacg cgtgatttgc gcaatcgcca ccgggtcaat aaagagttcg gcaaagccca
     6001 ttagcgccag cccggatatc atcacgccca ttgacgcttg accgccagcc gctgcatgtc
     6061 gggcatcaaa tgccaacaac ataaagccac aagccatcag cagtaagcca aaggcaaact
     6121 tcagccagac gcgcaatgtt gagttgccgc ggctttctgg cgacgccagc caggccagta
     6181 caaccccagc gagcatcacc gcaattgcat tcaccgactg gaatagtgct gtaggtactt
     6241 caatattgaa tgcctgacga ttcacaaagc gatcgataaa caagctgatg gtactgccgc
     6301 cctgttgtgc cagtacccag aacaatgtcc cgacaaacat caacaataca atttgccaaa
     6361 gagcacggcg gtgttcgggg aatttgatca tcatgcgggc aatgatttgt gcggcaatga
     6421 ggcaaacgat cgccagcaaa tatccggacc agtcgttctc cagcagcagg gtaaaaaata
     6481 ctggggctaa acagagcatc accaccaacc agctccatac cggtaaggca aatttgacgc
     6541 tagtgagcgc ttttttatcc atactacgcg tggactggaa atgacgatga ccgcttaaga
     6601 aaatcaacaa accgataaac atgccgccac ccgcaagggc aaagccaaca tgccatccat
     6661 accactgagc agccaggccg caggcgattg gggcggcgat agaaccgata ttgcccgcag
     6721 catacagcag cgaaaaaccg ccatcacgtc gatgatcgtt ctcgtcgtaa agctcgccaa
     6781 gcaaacagct gatgtttgat ttgaataaac cgtagccaca aataatgatt gccagcgcca
     6841 gatacaggct aaaggttgaa tttgtatcaa tgcccagcac cacatggcca agggtcatta
     6901 acagcgcgcc ggcaatcact gcagtgcggt tgccgagcag gcggtcggca agccagccgc
     6961 cgagaatagg ggtaacgtaa accagagaag catatgcgct gaacaggctg atggcatggt
     7021 tatcatcaaa accaagctga tgggtgagat agagaatgag taaggcacgc atgccgtaaa
     7081 aactgaagta ctcccagatt tggatcgcca cgatatagta tatcgcgcgc ggctgtgagg
     7141 gtgttttcat gtgttctcct tatgagcaaa aaagggaagt ggcaagccac ttcccttgta
     7201 cgagctaatt attttttgct ttcttctttc aataccttaa cggtatagcg gccatcagcc
     7261 tgacggtatg caccgtgaat atcggtttca aagcccggat agtgagcgcc gatttcacac
     7321 agcatctgca ggaactccag aaccggacgg ctttcttcgg tgatcatttc acccggcatt
     7381 accagaggaa ctcccggcgg atacggaagg atcatattgg cgttaatacg accgaccatt
     7441 tcgtcgaggt aaacttcttc ggtcataccg tgcagctctt tctggaacgc agcatacgga
     7501 gtcattacca tcgtcggcag cacttcaaat gcgcgataca tcagatccgg cagattgtgg
     7561 tgaacaatca gtttgtggat attctgagcc agttcctgaa tacgcatgtt ttcatagaat
     7621 tcaggatctt cacgatacag agacggcagc atgtttttca cacgcaggtt caggtcgaac
     7681 gcacgtttga agtcagtcag agcacgcagc aggctcagtg ctttggtctt atcgataccg
     7741 atgctgaaca ggaacagcag gttatacgga ccggttttct caacaacgat gccatgttcg
     7801 tcgaggtatt tcgccacgat gctggccgga ataccaaagt cgctcatggt gccgtctttt
     7861 tccatccccg gagtcagcag ggtgactttg atcgggtcaa gatacatgtg ctcgttatcg
     7921 atgtttttga agccgtgcca ggtgctgtca gaacgcagcg gccagcattc agtggtatcg
     7981 atatgatccg gctgccatac atcaaagaac cagccatcag attccgttct cagacgtttg
     8041 atctctttac ggaatttgat cgcacgttca atggaaccgt tgatcagacg cttacctgca
     8101 ttgcctttca tcatcgccgc agcggtttca gtggacgcca cgataccgta gtgcggagaa
     8161 gtggtggtgt gcatcatgta agcttcgtta aaggtttctt cgtttacgtc acctttaacg
     8221 tggatcatgg aagcctgaga gaacgccgcc agcagtttgt gagtagactg ggtttcgtaa
     8281 atcactttcc cttctacacg gccaccgctc ataccgcatt taccttcgta aatcggtgag
     8341 aagttggtgt aaggtaccca cgcggagtca aagtggatgg atttcacatc cagtgttttc
     8401 ttgatgaagt cggtgttgta cagcagacca tcataggtag agttagtaat tacagcatgt
     8461 accggccagg ttgcgtttgg tgtttctttc acgcgcttag caatggtagc gtgctggaat
     8521 tcactctgtg ggataccacc aagaataccg taagcgttac gggtcgggcg gaaatagatt
     8581 ggcgtaacat cgctcatcat catcaggtgg gtcagcgatt tgtggcagtt acggtcaatc
     8641 agaatggtgc tgcctgctgg agcagagtac ataccaacaa ttttgttcgc agtggaagta
     8701 ccgttggtca ccatgtagct gcggtctgcg ttaaagacgc gagcgatata ctgttctgct
     8761 tctttgtgtg gaccactgtg atccagcaga gaacccagtt cagatactga aatggaaata
     8821 tcagatttca tggtgttcgg accaaagaaa tcatagaaca ggctacctac cgggcttttc
     8881 tggaatgcag taccgcccat gtgaccagga gtacagaaag tatatttacc ttcacgaaca
     8941 tatttaaaca gtgctttagt cagcggaggc agaatagtgt tgatatattc gtcagtggtc
     9001 tgcttgatct tattagcaat atcttcagca gcacccagcg catattcaaa gaagctaatc
     9061 tgtaaacgca ggtcattcag gcttacatcg agagtggaat acgtattagc gaacgcgtac
     9121 aacggcaggt tctcgttcat tttgctaatt tcttcgcaca gctcgagatt atatttatcc
     9181 cagtcaaaaa taacgccgca cagacgcgca ttgttttcga tcagttttaa taagtcgtca
     9241 cggtcgttcg ggtaaacaat ctggaagttc agacgttcaa gcgcgcgatg aagttcacgg
     9301 atgggttctt ctttaaaata aacccccatg tgattcaata ttgcaataac gttcatagtc
     9361 atatctccag gtaaaaaagg cccctcccaa cacatgggac aaaatgaaag gaggagcctc
     9421 ggaaaatact tttaattaat gtgcgttaga cgcggtgtgg ttatccattg agtggctctg
     9481 gcgctcgtgc attttgcgag cgtagaacat caggataatc aggctgacga tgaaggtacc
     9541 tgccagctcg aaggagcttg cgcccatcag cgcgatgaag cagaacacgc aacccagtac
     9601 agagcagatc aggctgacaa agttgcggat gttaacgcct tcaaaacgaa tcaggtcaac
     9661 gcaagagtag aaatacggca gcatagtcag cagtactgcg ataccggtca gttcaccgaa
     9721 caggtcagat gctttaccac cggcagagtt catcagagtg ataaggatca tcagggcagt
     9781 cattttcact gcagccagca gcagaccttt tttcggaata ccgttgctgt cgacttcacc
     9841 ataaactttc gggaagttac cgtcgttagc ggcacgtaca cctgcctggc ctaccaacat
     9901 catccaggag cccagagaag tcaggcacgc aaaggcggtg aatgcagaaa ccagcggcgc
     9961 agcccagtta ccgaggatag ttgaagcact gattgcaaac ggagcaccgg aagccgccat
    10021 tacagaagac ggatacatac cggaaagcac ctgagtcgca gcgatgtaaa caatacctgc
    10081 taaaccagta cccagcatgg ttgccagcgg aacggtacgt ttcgggtttt taaccatacc
    10141 agtacttaca gctgcggatt caacacccac gaaggcccac aggcagagca gaatactttt
    10201 aatgatcgca tgaccatcag tggtatccgc agtattccag ttagctgcat aagttgccgc
    10261 atcaaaccaa tgccagccaa caatagcagt catcaccaca ggaataagaa ccagcaccag
    10321 accaatagtg gttaaacggc ttacccaagt accgccgagc atatttacaa aggtaaatac
    10381 ccagacgata gcaatacagg cgatacccgc cggaacagga tcatttaata ctgggaagaa
    10441 ggtggaaaga taagatacag cggtaatacc aatcgccagg ttaccaatcc agttagcatg
    10501 gtaataaaga acacctgtct gaaaaccaaa tgcaggggaa atttctccgg cataagcaat
    10561 tgggccacct tgttgcgggt tttttgttgc cagtcgggca tatacatacg ccagcgacat
    10621 tgcaccaata atagagataa tccaacccca gatagcaata ccaccgatac ttgctaggtt
    10681 cgcaggtaat aatgcaatac cgctccccat catattaccg gcaacaacac cggtacaggc
    10741 aaatagcccg atcttcttgg cagaactcat gctcttctcc taatttcatt tttgaatttg
    10801 gagtccgggt catgatgtat aactatttcc tgaccagacc aaactggcga taagattact
    10861 cacgaaaaaa ggattaatcc taaagattag gtgaaataac acaaaagttt ctgtaagtga
    10921 gaacttgag
//
LOCUS       DAPHBJ010000147        10925 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.149, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000147 DAPHBJ010000000
VERSION     DAPHBJ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.149"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            263..667
                     /gene="sdhC"
                     /locus_tag="RHZ76_003374"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             263..667
                     /gene="sdhC"
                     /locus_tag="RHZ76_003374"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDV2813964.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            661..1008
                     /gene="sdhD"
                     /locus_tag="RHZ76_003375"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             661..1008
                     /gene="sdhD"
                     /locus_tag="RHZ76_003375"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HDV2813965.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            1008..2774
                     /gene="sdhA"
                     /locus_tag="RHZ76_003376"
     CDS             1008..2774
                     /gene="sdhA"
                     /locus_tag="RHZ76_003376"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HDV2813966.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2790..3506
                     /gene="sdhB"
                     /locus_tag="RHZ76_003377"
     CDS             2790..3506
                     /gene="sdhB"
                     /locus_tag="RHZ76_003377"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HDV2813967.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3707..6508
                     /gene="sucA"
                     /locus_tag="RHZ76_003378"
     CDS             3707..6508
                     /gene="sucA"
                     /locus_tag="RHZ76_003378"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDV2813968.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6523..7740
                     /gene="odhB"
                     /locus_tag="RHZ76_003379"
     CDS             6523..7740
                     /gene="odhB"
                     /locus_tag="RHZ76_003379"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDV2813969.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            8015..9181
                     /gene="sucC"
                     /locus_tag="RHZ76_003380"
     CDS             8015..9181
                     /gene="sucC"
                     /locus_tag="RHZ76_003380"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDV2813970.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            9181..10050
                     /gene="sucD"
                     /locus_tag="RHZ76_003381"
     CDS             9181..10050
                     /gene="sucD"
                     /locus_tag="RHZ76_003381"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDV2813971.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(10154..10876)
                     /gene="mngR"
                     /locus_tag="RHZ76_003382"
     CDS             complement(10154..10876)
                     /gene="mngR"
                     /locus_tag="RHZ76_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HDV2813972.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIERSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
BASE COUNT         2568 a         2831 c         3016 g         2510 t
ORIGIN      
        1 acaaactata tgtaggttaa ttgtaatgat tttgtgaaca gcctatactg ccgccaggtc
       61 tccggaacac cctgcaatcc cgagccaccc agcgttgtaa cgtgtcgttt tcgcatctgg
      121 aagcagtgtt ttgcatgacg cgcagttata gaaaggacgc tgtctgaccc gcaagcagac
      181 cggaggaagg aaatcccgac gtctccaggt aacagaaagt taacctctgt gcccgtagtc
      241 cccagggaat aataagaaca gcatgtgggc gttattcatg ataagaaatg tgaaaaaaca
      301 aagacctgtt aatctggacc tacagaccat ccggttcccc atcacggcga tagcgtccat
      361 tctccatcgc gtttccggtg tgatcacctt tgttgcagtg ggcatcctgc tgtggcttct
      421 gggtaccagc ctctcttccc ctgaaggttt cgagcaagct tccgcgatta tgggcagctt
      481 cttcgtcaaa tttatcatgt ggggcatcct taccgctctg gcgtatcacg tcgtcgtagg
      541 tattcgccac atgatgatgg attttggcta tctggaagaa acattcgaag cgggtaaacg
      601 ctccgccaaa atctcctttg ttattactgt cgtgctttca cttctcgcag gagtcctcgt
      661 atggtaagca acgcctccgc attaggacgc aatggcgtac atgatttcat cctcgttcgc
      721 gctaccgcta tcgtcctgac gctctacatc atttatatgg tcggtttttt cgctaccagt
      781 ggcgagctga catatgaagt ctggatcggt ttcttcgcct ctgcgttcac caaagtgttc
      841 accctgctgg cgctgttttc tatcttgatc catgcctgga tcggcatgtg gcaggtgttg
      901 accgactacg ttaaaccgct ggccttgcgc ctgatgctgc aactggtgat tgtcgttgct
      961 ctggtggttt acgtgattta tggattcgtt gtggtgtggg gtgtgtgatg aaattgccag
     1021 tcagagaatt tgatgcagtt gtgattggtg ccggtggcgc aggtatgcgc gcggcgctgc
     1081 aaatttccca gagcggtcaa acctgtgcgc tgctctctaa agtcttcccg acccgttccc
     1141 ataccgtttc tgcgcaaggc ggcattaccg ttgcgctggg taatacccat gaagataact
     1201 gggaatggca tatgtacgac accgtaaaag gttcggacta tatcggtgac caggacgcga
     1261 ttgaatatat gtgtaaaacc gggccggaag cgattctgga actcgaacac atgggcctgc
     1321 cgttctcgcg tcttgatgat ggtcgtatct atcaacgtcc gtttggcggt cagtcgaaaa
     1381 acttcggcgg cgagcaggcg gcacgcactg cggcagcagc tgaccgtacc ggtcacgcac
     1441 tgttgcatac cctttatcag cagaacctga aaaaccacac cactattttc tccgagtggt
     1501 atgcgctgga tctggtgaaa aaccaggatg gcgcggtggt gggttgtact gcactgtgca
     1561 tcgaaactgg tgaagtggtt tatttcaaag cccgcgctac tgtgctggcg actggcgggg
     1621 cagggcgtat ttatcagtcc accactaacg cccacattaa cactggcgac ggcgtcggca
     1681 tggctatccg cgcaggcgta ccggtgcagg atatggaaat gtggcagttc cacccgaccg
     1741 gtattgccgg tgcgggcgta ctggtcaccg aaggttgccg tggtgaaggc ggttatctgc
     1801 tgaacaaaca tggcgaacgc tttatggagc gttatgcgcc gaacgccaaa gacctggcgg
     1861 gccgtgacgt ggtggcgcgt tccatcatga ttgaaatccg tgaaggtcgc ggctgtgatg
     1921 gtccgtgggg gccacatgcg aaactgaaac tcgaccacct gggtaaagaa gttctcgaat
     1981 cccgtctgcc gggtattctg gaactctccc gtaccttcgc tcacgttgat ccggtgaaag
     2041 agccgattcc ggttatccca acctgtcact acatgatggg tggtattccg accaaagtga
     2101 ccggtcaggc gctgacggtg aacgagaaag gcgaagatgt ggttgttccg gggctgtttg
     2161 ccgttggtga aatcgcttgt gtatcggtac acggcgctaa ccgtctgggc ggcaactcgc
     2221 tgctggacct ggtggtcttt ggtcgcgcag cgggtctgca tctgcaagag tctatcgccg
     2281 agcagggcgc attgcgcgat gccagcgagt ctgatgttga agcatctctg gatcgtctga
     2341 accgctggaa caataatcgt aacggtgaag atccggtggc gattcgtaaa gcgctgcaag
     2401 aatgtatgca gcataacttc tcggtcttcc gtgaaggtga tgcgatggcg aaagggcttg
     2461 agcagttgaa agttatccgc gagcgtctga aaaatgcccg tctggatgac acttccagcg
     2521 agttcaacac ccagcgcgtt gagtgcctgg aactggataa cctgatggaa acggcgtatg
     2581 caacggctgt ttctgccaac ttccgtaccg aaagccgtgg cgcgcatagc cgcttcgact
     2641 tcccggatcg tgatgatgaa aactggctgt gccactccct gtatctgcca gagtcggaat
     2701 ccatgacgcg ccgaagcgtc aacatggaac cgaaactgcg cccggcattc ccgccgaaga
     2761 ttcgtactta ctaatgcgga gacaggaaaa tgagactcga gttttcaatt tatcgctata
     2821 acccggatgt tgatgatgct ccgcgtatgc aggattacac cctggaagcg gaagaaggtc
     2881 gcgacatgat gttgctggat gcgcttatcc agctgaaaga gaaagatccc agcctgtcgt
     2941 tccgccgctc ctgccgtgaa ggtgtgtgcg gttccgacgg tctgaacatg aacggcaaga
     3001 atggtctggc ctgtattacc ccgatttcgg cactcaacca gccgggcaag aagattgtga
     3061 ttcgcccgct gccaggttta ccggtgatcc gcgatttggt ggtagacatg ggacaattct
     3121 atgcgcaata tgagaaaatt aagccttacc tgttgaataa tggacaaaat ccgccagctc
     3181 gcgagcattt acagatgcca gagcagcgcg aaaaactcga cgggctgtat gaatgtattc
     3241 tctgcgcatg ttgttcaacc tcttgtccgt ctttctggtg gaatcccgat aagtttatcg
     3301 gcccggcagg cttgttagcg gcatatcgtt tcctgatcga tagccgtgat accgagactg
     3361 acagccgcct cgacggtttg agcgatgcat tcagtgtatt ccgctgtcac agcatcatga
     3421 actgcgtcag tgtatgtccg aaggggctga acccgacgcg cgccatcggc catatcaagt
     3481 cgatgttgtt gcaacgtaat gcgtaattaa atgttgatgc cggggaaatc ccccggctat
     3541 tcaggaaatc tttaaaaact gcccctgaca ctaagacagt ttttaaaggt tccttcgcga
     3601 gccactacgt agacaagagc tcgcaagtga accccggcac gcacatcact gtgcgtggta
     3661 gtatccacgg cgaagtaagc ataaaaaaga tgcttaaggg atcacgatgc agaacagcgc
     3721 tttgaaagcc tggttggact cttcttacct ctctggcgca aaccagagct ggatagaaca
     3781 gctctatgaa gacttcttaa ccgatcctga ctcggttgac gctaactggc gttcgacgtt
     3841 ccagcagtta cctggtacgg gagtcaaacc ggatcaattc cactctcaaa cgcgtgaata
     3901 tttccgccgc ctggcgaaag acgcttcacg ttactcttca acgatctccg accctgacac
     3961 caatgtgaag caggtaaaag tcctgcagct cattaacgca taccgcttcc gtggtcacca
     4021 gcatgcgaat ctcgatccgc tgggactgtg gcagcaagat aaagtggccg atctggatcc
     4081 gtctttccac gatctgaccg aagcagactt ccaggagacc ttcaacgtcg gttcatttgc
     4141 cagcggcaaa gaaaccatga aactcggcga gctgctggaa gccctcaagc aaacctactg
     4201 cggcccgatt ggtgccgagt atatgcacat caccagcacc gaagaaaaac gctggatcca
     4261 acagcgtatc gagtctggtc gcgcgacttt caatagcgaa gagaaaaaac gcttcttaag
     4321 cgaactgacc gccgctgaag gccttgaacg ttacctcggc gcaaaattcc ctggcgcaaa
     4381 acgcttctcg ctggaaggcg gtgacgcgtt aatcccgatg cttaaagaga tgatccgcca
     4441 cgctggcaac agcggcaccc gcgaagtggt tctcgggatg gcgcaccgtg gtcgtctgaa
     4501 cgtgctggtg aacgtgctgg gtaaaaaacc gcaagatttg ttcgacgagt tcgccggtaa
     4561 acataaagaa cacctcggca cgggtgacgt gaaataccac atgggcttct cgtctgactt
     4621 ccagaccgat ggcggcctgg tgcacctggc gctggcgttt aacccgtctc accttgagat
     4681 tgtaagcccg gtagttatcg gttctgttcg tgcccgtctg gacagacttg atgagccgag
     4741 cagcaacaaa gtgctgccaa tcaccatcca cggtgacgcc gcagtgaccg ggcagggtgt
     4801 ggttcaggaa actctgaaca tgtcgaaagc gcgtggttat gaagttggcg gtacggtacg
     4861 tatcgttatc aacaaccagg ttggtttcac cacctctaat ccgctggatg ctcgttctac
     4921 accgtactgt actgatatcg gtaagatggt tcaggcaccg attttccacg ttaacgcgga
     4981 tgatccggaa gccgttgcct ttgtgacccg tctggcgctc gatttccgta acacctttaa
     5041 acgtgatgtc ttcatcgacc tggtgtgcta ccgccgtcac ggccacaacg aagccgacga
     5101 gccgagcgca acccagccgc tgatgtatca gaaaatcaaa aaacatccga cgccgcgcaa
     5161 aatctatgct gacaagctgg agcaggaaaa agtggcgacg ctggaagatg ccaccgagat
     5221 ggttaacctg taccgcgatg cgctggatgc tggcgattgc gtagtggcag agtggcgtcc
     5281 gatgaacatg cactctttca cctggtcgcc gtatctcaac cacgaatggg acgaagagta
     5341 cccgaacaaa gttgagatga agcgcctgca ggaactggct aaacgcatca gcacggtgcc
     5401 ggaagcggtt gaaatgcagt ctcgcgttgc caaaatttat ggcgatcgcc aggcgatggc
     5461 tgccggtgag aaactgttcg actggggcgg cgcggaaaac ctcgcttacg ccacgctggt
     5521 tgacgaaggc attccggttc gcctgtcggg tgaagactcc ggtcgcggta ccttcttcca
     5581 ccgccacgcg gtgatccaca accagtctaa cggttccact tacacgccgc tgcaacacat
     5641 ccataacggc cagggcgcgt tccgtgtctg ggactccgta ctgtctgaag aagcagtgct
     5701 ggcgtttgaa tacggttatg ccaccgcaga accacgcact ctgactatct gggaagcgca
     5761 gttcggtgac ttcgccaacg gtgcgcaggt ggttatcgac cagttcatct cctctggcga
     5821 acagaaatgg ggccggatgt gtggtctggt gatgttgctg ccgcacggtt atgaagggca
     5881 ggggccggag cactcctccg cgcgtctgga acgttatctg caactttgtg ctgagcaaaa
     5941 catgcaggtg tgcgtaccgt ctaccccggc acaggtttac cacatgctgc gtcgtcaggc
     6001 gctgcgcggg atgcgtcgtc cgctggtcgt gatgtcgccg aaatccctgc tgcgtcatcc
     6061 gctggcggtt tccagcctcg aagaactggc gaacggcacc ttcctgccag ccatcggtga
     6121 aatcgacgag cttgatccga agggtgtgaa gcgcgtagtg atgtgttctg gtaaggttta
     6181 ttacgacctg ctggaacaac gtcgtaagaa caatcaacac gatgtcgcca ttgtgcgtat
     6241 cgagcaactc tacccgttcc cacataaagc gatgcaggaa gtgttgcagc agtttgctca
     6301 cgtcaaggat tttgtctggt gccaggaaga gccgctcaac cagggcgcat ggtactgcag
     6361 ccagcatcat ttccgtgaag tgattccgtt tggggcttct ctgcgttatg caggccgccc
     6421 ggcctccgcc tctccggcgg tagggtatat gtccgttcac cagaaacagc aacaagatct
     6481 ggttaatgac gcgctgaacg tcgaataaat aaaggataca caatgagtag cgtagatatt
     6541 ctggtccctg acctgcctga atccgtagcc gatgccaccg tcgcaacctg gcataaaaaa
     6601 cccggcgacg cagtcgtacg tgatgaagtg ctggtagaaa tcgaaactga caaagtggta
     6661 ctggaagtac cggcatcagc agacggcatt ctggatgcgg ttctggaaga tgaaggtaca
     6721 acggtaacgt ctcgtcagat ccttggtcgc ctgcgtgaag gcaacagcgc cggtaaagaa
     6781 accagcgcca aatctgaaga gaaagcgtcc actccggcgc aacgccagca ggcgtctctg
     6841 gaagagcaaa acaacgatgc gttaagcccg gcgatccgtc gcctgctggc tgaacacaat
     6901 ctcgacgcca gcgccattaa aggcaccggt gtgggtggtc gtctgactcg tgaagatgtg
     6961 gaaaaacatc tggcgaaagc cccggcgaaa gagtctgctc cggcagcggc tgctccggcg
     7021 gcgcaaccgg ctctggctgc acgtagtgaa aaacgtgtcc cgatgactcg cctgcgtaag
     7081 cgtgtggcag agcgtctgct ggaagcgaaa aactccaccg ccatgctgac cacgttcaac
     7141 gaagtcaaca tgaagccgat tatggatctg cgtaagcagt acggtgaagc gtttgaaaaa
     7201 cgccacggca tccgtctggg ctttatgtcc ttctacgtga aagcggtggt tgaagccctg
     7261 aaacgttacc cggaagtgaa cgcttctatc gacggcgatg acgtggttta ccacaactat
     7321 ttcgacgtca gcatggcggt ttctacgccg cgcggcctgg tgacgccggt tctgcgtgat
     7381 gtcgataccc tcggcatggc agacatcgag aagaaaatca aagagctggc agtcaaaggc
     7441 cgtgacggca agctgaccgt tgaagatctg accggtggta acttcaccat caccaacggt
     7501 ggtgtgttcg gttccctgat gtctacgccg atcatcaacc cgccgcagag cgcaattctg
     7561 ggtatgcacg ctatcaaaga tcgtccgatg gcggtgaatg gtcaggttga gatcctgccg
     7621 atgatgtacc tggcgctgtc ctacgatcac cgtctgatcg atggtcgcga atccgtgggc
     7681 ttcctggtaa cgatcaaaga gttgctggaa gatccgacgc gtctgctgct ggacgtgtag
     7741 tagtttaagt ttcacctgca ctgtagaccg gataaggcat tatcgccttc tccggcaatt
     7801 gaagcctgat gcgacgctga cgcgtcttat caggcctacg ggaccaccaa tgtaggtcgg
     7861 ataaggcgct agcgccgcat ccgacaagcg atgcctgatg tgacgtttaa cgtgtcttat
     7921 caggcctacg ggtgaccgac aatgcccgga agcgatacga aatattcggt ctacggttta
     7981 aaagataacg attactgaag gatggacaga acacatgaac ttacatgaat atcaggcaaa
     8041 acaacttttt gcccgctatg gcttaccagc accggtgggt tatgcctgta ctactccgcg
     8101 cgaagcagaa gaagccgctt caaaaatcgg tgccggtccg tgggtagtga aatgtcaggt
     8161 tcacgctggt ggccgcggta aagcgggcgg tgtgaaagtt gtaaacagca aagaagacat
     8221 ccgtgctttt gcagaaaact ggctgggcaa gcgtctggta acgtatcaaa cagatgccaa
     8281 tggccaaccg gttaaccaga ttctggttga agcagcgacc gatatcgcta aagagctgta
     8341 tctcggtgcc gttgttgacc gtagttcccg tcgtgtggtc tttatggcct ccaccgaagg
     8401 cggcgtggaa atcgaaaaag tggcggaaga aactccgcac ctgatccata aagttgcgct
     8461 tgatccgctg actggcccga tgccgtatca gggacgcgag ctggcgttca aactgggtct
     8521 ggaaggtaaa ctggttcagc agttcaccaa aatcttcatg ggcctggcga ccattttcct
     8581 ggagcgcgac ctggcgttga tcgaaatcaa cccgctggtc atcaccaaac agggcgatct
     8641 gatttgcctc gacggcaaac tgggcgctga cggcaacgca ctgttccgcc agcctgatct
     8701 gcgcgaaatg cgtgaccagt cgcaggaaga tccgcgtgaa gcacaggctg cacagtggga
     8761 actgaactac gttgcgctgg acggtaacat cggttgtatg gttaacggcg caggtctggc
     8821 gatgggtacg atggacatcg ttaaactgca cggcggcgaa ccggctaact tccttgacgt
     8881 tggcggcggc gcaaccaaag aacgtgtaac cgaagcgttc aaaatcatcc tctctgacga
     8941 caaagtgaaa gccgttctgg ttaacatctt cggcggtatc gttcgttgcg acctgatcgc
     9001 tgacggtatc atcggcgcgg tagcagaagt gggtgttaac gtaccggtcg tggtacgtct
     9061 ggaaggtaac aacgccgaac tcggcgcgaa gaaactggct gacagcggcc tgaatattat
     9121 tgcagcaaaa ggtctgacgg atgcagctca gcaggttgtt gccgcagtgg aggggaaata
     9181 atgtccattt taatcgataa aaacaccaag gttatctgcc agggctttac cggtagccag
     9241 gggactttcc actcagaaca ggccattgca tacggcacta aaatggttgg tggcgtaact
     9301 ccaggtaaag gcggcactac ccacctcggc ctgccggtgt tcaacaccgt gcgtgaagct
     9361 gttgctgcta ctggcgctac cgcttctgtt atctacgtac cagcaccgtt ctgcaaagac
     9421 tccattctgg aagccatcga cgcaggcatc aaactgatta tcaccatcac tgaaggcatc
     9481 ccgacgctgg atatgctgac tgtgaaagtg aaactggacg aagcaggcgt tcgtatgatc
     9541 ggcccgaact gcccaggcgt tatcactccg ggtgaatgca aaatcggtat ccagccgggt
     9601 cacattcaca aaccgggtaa agtgggtatc gtttcccgtt ccggtacact gacctatgaa
     9661 gcggttaaac agaccacgga ttacggtttc ggtcagtcga cctgtgtcgg tatcggcggt
     9721 gacccgatcc ctggctctaa ctttatcgac attctcgaaa tgttcgaaaa agatccgcag
     9781 accgaagcga tcgtgatgat tggtgagatc ggcggtagcg ctgaagaaga agcggcagcg
     9841 tacatcaaag agcacgttac caagccagtt gtgggctaca ttgctggtgt gactgcgccg
     9901 aaaggcaaac gtatgggcca cgcgggtgcc atcattgccg gtgggaaagg gactgcggat
     9961 gagaaattcg ctgctctgga agccgcaggc gtgaaaaccg ttcgcagcct ggcggatatc
    10021 ggtgaagcac tgaaaactgt tctgaaataa atatctgtaa taagaaatag ccctcgccgc
    10081 ttccctctac aggaatggcg aagggctgtc ggtttcgaca tggttggcca tcgtatgatg
    10141 gccttttttg tgcttatcgc gatgattttc gctgcgctat cagggtaaat ttatagtcat
    10201 cggtattaaa agcgttgcgg ctatattcaa acacccgacc atcaactaaa tatccacgcg
    10261 atactttttc aagaatcggc tttgtctggc tgatattaag cagacggctc atctcttcgg
    10321 ttggcatcag aggaatgatt tcctgttcgc tacgctcgat aaccattttc ttcacttctt
    10381 caataaagtg atatttcgaa ttttccatga cctgccaggt gagatccggg aacaacgcaa
    10441 gcggcatcca ggtttcttcc agcgccattg gcttttgctt gcgatagcgc acgcgcttca
    10501 catgccacac acgatcctgc gcggtgattt gtagctgttg ctgaagaaaa tcgtcagccg
    10561 gaatcacttc aaatatcagg acttcactgt gtgtatcgac gtgacggtcc gacagttttt
    10621 catcaaaact ggttaactga aaaatatcgt aattgacccg ctcttctttg acgtaagtcc
    10681 cgctgccctg aatgctttcg aggatctgct gctcgactag ctggcgcaaa gcctgacgca
    10741 ccgtaacccg gctgacgcca aactctgttt gtaatgcaga ttcggtaggt aacgcatcgc
    10801 caggtttaag ctccccacgc gcaatttgct cacgaatgcg atcggcaatc tgccggtata
    10861 agggtttgtg tcccattttt agtatctcat taatacgaat ttagccatta tgcccgataa
    10921 attca
//
LOCUS       DAPHBJ010000148        10913 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.150, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000148 DAPHBJ010000000
VERSION     DAPHBJ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.150"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..822
                     /locus_tag="RHZ76_003383"
     CDS             <1..822
                     /locus_tag="RHZ76_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HDV2813973.1"
                     /translation="IVGLYNDTIKLNLHFEWTNKNNITLSNNQTSFTSGYSVTVTPAA
                     SNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGGKSWDACVNSYRNA
                     LAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGNKATINSKQGDIIL
                     RCKNLLGQQNQTSRKMQVYLSISDLLTNSNTILKGAEDNGVGFILESNGSPVTLLNIT
                     NSSKGYTNLKEVAAKSKLTDTTVSIPITASYYVYDTNKVKSGALEATALINVKYD"
     gene            complement(904..1242)
                     /gene="rcnB"
                     /locus_tag="RHZ76_003384"
     CDS             complement(904..1242)
                     /gene="rcnB"
                     /locus_tag="RHZ76_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HDV2813974.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1461..2285)
                     /gene="rcnA"
                     /locus_tag="RHZ76_003385"
     CDS             complement(1461..2285)
                     /gene="rcnA"
                     /locus_tag="RHZ76_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HDV2813975.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHGHDYEHHHHDHEHHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            2406..2678
                     /gene="rcnR"
                     /locus_tag="RHZ76_003386"
     CDS             2406..2678
                     /gene="rcnR"
                     /locus_tag="RHZ76_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HDV2813976.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      2770..2866
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2901..3689
                     /gene="thiM"
                     /locus_tag="RHZ76_003387"
     CDS             2901..3689
                     /gene="thiM"
                     /locus_tag="RHZ76_003387"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDV2813977.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            3686..4486
                     /gene="thiD"
                     /locus_tag="RHZ76_003388"
     CDS             3686..4486
                     /gene="thiD"
                     /locus_tag="RHZ76_003388"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDV2813978.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            4551..5369
                     /locus_tag="RHZ76_003389"
     CDS             4551..5369
                     /locus_tag="RHZ76_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HDV2813979.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIIAIYPRQTVNFFYSTAVQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGMLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            5421..6167
                     /locus_tag="RHZ76_003390"
     CDS             5421..6167
                     /locus_tag="RHZ76_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV2813980.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(6141..7106)
                     /locus_tag="RHZ76_003391"
     CDS             complement(6141..7106)
                     /locus_tag="RHZ76_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDV2813981.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(7103..8107)
                     /locus_tag="RHZ76_003392"
     CDS             complement(7103..8107)
                     /locus_tag="RHZ76_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDV2813982.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPNLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPIAELENNGVTNGAAMRVSPLGCLLPAHDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(8104..9381)
                     /locus_tag="RHZ76_003393"
     CDS             complement(8104..9381)
                     /locus_tag="RHZ76_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HDV2813983.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            9638..10690
                     /gene="fbaB"
                     /locus_tag="RHZ76_003394"
     CDS             9638..10690
                     /gene="fbaB"
                     /locus_tag="RHZ76_003394"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HDV2813984.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
BASE COUNT         2837 a         2738 c         2773 g         2565 t
ORIGIN      
        1 attgttggac tttataacga taccataaaa ttaaatttac attttgaatg gaccaataaa
       61 aacaacatca cgttgtcaaa taatcagacc agtttcacca gtggttattc agttacggtg
      121 acacctgcgg ccagtaatgc aaaagtgaat gtttctgcgg ggggcggcgg ttcagtgatg
      181 attaatggtg ttgcgacatt atccagtgct tcatcatcga cacgcgggag tgccgcagta
      241 caatttctac tgtgtttatt aggtggcaag tcatgggatg catgtgtaaa tagctacaga
      301 aatgcattgg cacaaaatgc aggtgtctat tcctttaatc tgacattgtc atacaacccg
      361 ataaccacaa cctgcaaacc ggacgattta ttaattactt tagacagtat tcccgtttca
      421 caattaccag ccacaggtaa caaagcaaca ataaatagta aacaagggga tattattctg
      481 cgttgtaaaa atttattagg tcaacaaaat caaacatcac ggaaaatgca ggtgtattta
      541 tcaatttctg acttgttaac caacagcaac acaatactga aaggtgcgga agataatggc
      601 gtaggattta ttcttgaaag taatggttcg ccagtcacac ttttaaatat cactaacagc
      661 agtaaaggat atacaaattt aaaggaagtt gcggcgaagt caaaacttac agatacaacg
      721 gtttcaattc cgataacagc cagttactac gtctacgata caaacaaagt taaatctggc
      781 gcactggagg caaccgcatt aatcaacgtg aaatacgact aataacaagc aagacgagca
      841 agtggctaat aataaaaaat aacaaggtgt ctgcatttcc ccctcatgat gagggggctt
      901 tttttagcga tgataaaaaa tctcaccgtc gtaggcttta atgattttac cgtcggtgtc
      961 gctgatcaac acgtacgcgc cacccatata ggtccagtgc gtcccggcat caggcgcggg
     1021 caggttacgc aactgccact gcttaatgtt gtactcatcg gtacggtaca tttctggtac
     1081 ggtgtcccct ggtttgaaat gcttaaagtc agcaatgaaa ctacttaact catacttaga
     1141 aattcccgag gtattggtcg aacccgcggt ggctggtgcg gcccaggcgg cactggtaac
     1201 cagcaaaagc gcacccagca acatcttatt tttaatggtc ataaactctc cactttgggg
     1261 atctgtaata tcagtcgcat atctttcctg ccatacaata taactgacaa gcgatatttt
     1321 gttagtgatc ccatgatttc gtaaaaagtg taaatgaaag gatgccctaa aagcatcctt
     1381 agcgagcaga gagtcagcgg aaatagctga tttaagaatc atcctgggct gaagataaca
     1441 tgcaagccta aaggattgag ttatcgcatt atgcccatga agccatgtac gcccatatac
     1501 acaccgacta agccaatcaa cagactggaa aaataggggg cgcgtttagc gagagtgtta
     1561 aatccgctcc agcgttttgc gacctgctga acgctgattg ctgcgccaac gcctacggtg
     1621 acaagcgtta acgccaggcc aatgctgaaa ctgacgacca gtgttgcgcc cagtgtcagg
     1681 gctttcaact gaatgcatat caacagcacg gtaattgctg ccgggcaggg gataaggcca
     1741 ccggttaagc caaataacaa aatttgccag ttggtgacct ctctaccatc aaagcgtcgt
     1801 ttaatgtcat tggcatgggc tcgtgcatgg gcatcctgat actcgccatg ttcgtgatgg
     1861 tgatgatgtc catggtcgtg gtgatgttcg tgatcgtgat gatgatgctc ataatcatgc
     1921 ccgtgcatat tctccagcca gttgcgttcg ccgcgccagg tacgccagaa catccagaac
     1981 gcggtgctaa taatgatcac tgcggaaatc agctggagcc acggttctgc tgattgagca
     2041 gtaaagcgct tgctgatcac catcccgcca aaggcaatta accagaccac tgcggtatgc
     2101 gaaatagttg ctgccagtcc gagcatcacc gcttgtttaa tggtgccttt gatggcgatg
     2161 ataaacgccg ccatcatcgt ttttgagtgc cctggttcca ggccatgaag cgcaccaagt
     2221 aagatggcgc tggggatgaa gaaccaggcg tttccttgct gaagaagagt tgtaaattcg
     2281 gtcatgataa taattcttag tattaattcg gcaatctgat tctactcccc cccagtacct
     2341 tatactaccc cccagtagat taatagtgct atgatttttc atattcttgt taaccaggtg
     2401 ttgccatgtc tcatacaatc cgtgataaac agaaactgaa agcgcgtgcc agtaagattc
     2461 agggccaggt cgtggcgctc aagaaaatgc tcgacgagcc gcacgaatgc gctgcagttt
     2521 tacaacagat tgctgctatc cgtggcgcgg taaacggtct gatgcgggaa gtgattaaag
     2581 gtcatctgac ggaacacatc gttcaccagg gggatgagct aaaacgtgaa gaagatctgg
     2641 atgtcgttct gaaggtgctg gattcatata tcaaataatt tattaacgcg attgtaaaac
     2701 tgccgttttt cctcgtttac aacgcgtgcg ctggacatta ccatcctcct ctgcgattta
     2761 tcatcgcaac caaacgactc ggggtgccct tctgcgtgaa ggctgagaaa tacccgtatc
     2821 acctgatctg gataatgcca gcgtagggaa gtcacggacc accaggtcat tgcttcttca
     2881 cgttatggca ggagcaaact atgcaagtcg acctgctggg ttcagcgcaa tctgcgcacg
     2941 cgttacacct ttttcaccaa cattcccctc ttgtgcactg catgaccaat gatgtggtgc
     3001 aaacctttac cgccaatacc ttgctggcgc tcggtgcatc gccagcgatg gttatcgaaa
     3061 ccgaagaggc cagtcagttt gcggctatcg ccagtgcctt gttgattaac gttggcacac
     3121 tgacgcagcc acgcgctcag gcgatgcgtg ctgccgttga gcaagcaaaa agctctcaaa
     3181 caccctggac gcttgatcca gtagcggtgg gtgcgctcga ttatcgccgc catttttgtc
     3241 atgaactttt atcttttaaa ccggcagcga tacgtggtaa tgcttcggaa atcatggcat
     3301 tagctggcat tgctaatggc ggacggggag tggataccac tgacgccgca gctaacgcga
     3361 tacccgctgc acaaacactg gcacgggaaa ctggcgcaat cgtcgtggtc actggcgaga
     3421 tggattatgt taccgatgga catcgtatca ttggtattca cggtggtgat ccgttaatga
     3481 ccaaagtggt aggaactggc tgtgcattat cggcggttgt cgctgcctgc tgtgcgttac
     3541 caggcgatac gctggaaaat gtcgcatctg cctgtcactg gatgaaacaa gccggagaac
     3601 gcgcagtcgc cagaagcgag gggccaggca gttttgttcc acatttcctt gatgcgctct
     3661 ggcaattgac gcaggaggtg caggcatgaa acgaattaac gctctgacga ttgccggtac
     3721 tgatccgagt ggtggtgcgg ggattcaggc cgatcttaaa accttctcgg cacttggcgc
     3781 ttatggttgc tcagttatta ctgcactggt ggcgcaaaat acccgtggcg tacagtcggt
     3841 gtatcgcatt gagcctgatt ttgtcgccgc ccagctcgat tcggtgttca gcgatgtgcg
     3901 aatcgatacc actaaaatcg gtatgctggc ggaaaccgat attgttgaag cggtggcaga
     3961 acggttgcaa cgttatcaga tccaaaacgt ggtactcgac accgttatgc tggcaaaaag
     4021 cggcgacccg ctgctttcac cttcggcggt tgctacgctg cgcagtcgat tattgccaca
     4081 ggtttcatta ataacgccaa acttgcccga agctgccgcc ttgctcgacg cgccacacgc
     4141 gcgcaccgaa caggaaatgc tggaacaagg gcgatcgctg ttggcgatgg gctgtggcgc
     4201 agtgctaatg aaaggtggtc atctggatga tgagcaaagc ccggactggc tgtttacccg
     4261 cgagggtgaa caacggttta ccgcaccgcg cattatgacc aaaaacaccc acggcactgg
     4321 ttgtacactc tctgcggcgt tggctgcact acgcccgcgc catacaaact gggctgacac
     4381 cgtacaggag gcaaaaagct ggctttcatc ggcgttagcc caggccgaca cgctggaagt
     4441 tggtcacggt attggtccgg ttcaccactt ccacgcctgg tggtgagcac cgtactatag
     4501 ctgctttggt tattgtcagc ctttggcact gacattcagg gatgaaacca atgcagctaa
     4561 gaattaccag tcgaaaaaaa ctcacctctc tattatgcgc tcttgggcta atttccatca
     4621 ttgcaattta tccgcgtcag acggtgaatt ttttctattc gacagcagtt cagattaaag
     4681 actacatcca cttctacggt tatcgcccgg ttaaatcttt cgcaattcgc attcctgcca
     4741 gttataccat tcacggaata gatgtttcgc gctggcagga gcggatcgac tggcagcggg
     4801 tggcaaaaat gcgcgacaac ggtatccgct tacagtttgc ttttattaag gcgacggaag
     4861 gcgaaaagct ggtggacccc tatttttcgc gtaactggca actaagccgc gaaaatggca
     4921 tgctgcgtgg ggcgtatcat tatttctccc cgtcggtatc cgcttcagtt caggcgcgat
     4981 tatttctgca aacggtggat ttctcacaag gcgatttccc tgccgtgctg gacgtagaag
     5041 aacggggaaa attatcggca aaagaattac gcaagcgggt aagtcagtgg ctaaaaatgg
     5101 tagaaaaacg cacgggaaaa aagccgatta tttactcagg agccgttttt tatcacacga
     5161 atctggcggg ctattttaat gaatatccgt ggtgggtggc tcactattat caacgtcgtc
     5221 cggacaatga cggaatggcc tggcgcttct ggcagcattc cgaccgtgga caggtagatg
     5281 gcatcaatgg tccggtggat tttaatgtgt ttaatggcac ggtggaggag ttgcagggat
     5341 tcgttgatgg gattaaagaa acgccttaaa taagaagcag tgatatactc ccaccagact
     5401 ccctggcagg aaaaagacaa atggaacaag cgcataccca gctgattgcc caactgaacg
     5461 agcgtatttt agctgcggat aacacgccgc tttatattaa gtttgccgaa acggtaaaaa
     5521 atgccgtgcg cagcggggtg ctggagcatg gcaatatttt gcccggtgag cgtgatttaa
     5581 gtcagttaac cggcgtttcg cgcattacgg tgcgcaaggc gatgcaggcg ctggaagaag
     5641 agggtgtggt gacgcgttcg cgcggttacg gcacgcagat caacaacatc ttcgaatact
     5701 cgttgaaaga agcgcgaggt ttttctcagc aggtggtatt gcgcgggaaa aagcccgata
     5761 cgctatgggt taacaagcgt gtcgtgaaat gtcccgaaga agtcgcgcag cagctggcgg
     5821 tcgaagcagg aagtgatgtc tttttgctta agcgtattcg ctatgtcgat gaagaagcgg
     5881 tatcgattga ggaatcgtgg gttccggcgc atttaattca tgatgttgat gccatcggga
     5941 tttcacttta tgactatttc cgcagccagc acatttaccc acaacgtaca cgttcccgcg
     6001 ttagcgcccg gatgccggat gccgagtttc agtcacatat tcagttagat agcaaaatac
     6061 cggtgctggt gatcaagcaa gtggcgcttg accaacagca acggcctatt gagtacagca
     6121 ttagccactg tcgcagcgat ctatacgttt ttgtgtgcga ggagtagttc ctcgcgcgtt
     6181 ggcgcacaat caccgccccg atgcccgaca acccacgacg ccactgcatt gcccagtaat
     6241 acggcatccg ccagtggcag accagaggcc agcccggcaa gtacgccacc tgcatgactg
     6301 tcgcccgccc caatggtgtc tacaacttgc gttggaaatg ccggaatgca gccagaagcg
     6361 tcgttgctga aataccatgc accctcttta tcgagacgaa cgatcaacgg cgcggcaaat
     6421 ttctcctgcc attgcttgcc aagtgttgtt atctctgcgg ataaagcaaa acgttcggcg
     6481 gcaatctcag cctcttgacg attgagtgat actaaaggtc gacaggccat gatccgcgcc
     6541 agtaatgcgt ccgggatatc gccgatacgt gggccaaaat cgataaacgg cgtcacgtct
     6601 tgcagctttt ccagccattc cactaacaat tcgccgcagg gcgaggccag ttgataaccg
     6661 gaaaaataga gcaggctgcc aggcgcaacg gttaatcgcg ccagccactg gcgattccac
     6721 tgattttcca caccgctgaa tgacataaaa gtgcgttcgc catccggctc aaccagcgcc
     6781 agacaccagc cgttatcgcc ttcggcgtta tcgatcaggc tgattaaccc ctctttcgcc
     6841 atccggttgc gaataatctc cgcccacacg ccctgaccga gcggcaaggc attacccgct
     6901 tcgatgccga ggcgctttaa cgccacggca atattcagtg cgcagccgcc aacgttaacg
     6961 ctctgctgct tcagttcgat atcgcaccca cgccagggga gggcataagc gtcggcgatc
     7021 acatcaatga ccgccgcgcc gacgaccatc accggctgac gcgaggttaa ttcaggcagc
     7081 agcgtgtgta atcgagcgcc gctcataccg cctcccgttg ttgacgatat ttcgccagcg
     7141 ctgtggcata gcggttgaaa tcaagctgat ttaccgcatc cagttccgcc tttaatgcag
     7201 gatcgatagc gttaacgcca tgcaacgcgc cgcaaatcgc cgtcgccata gcgccgatgg
     7261 tgtctgtgtc gccgccaagg ttagcgcaca ggacggcgca gcgattcggg tcggtttgtg
     7321 ccagttcaac cagcgcaatg gcgcacggaa cggactcaat agtgctggta cctgcgccaa
     7381 cgacctggta aagctgttcg ctggcggatt cggtgccgtc ggcattgcgc acaattttca
     7441 gcgcaatctc cagacgtgct gccagtgagg cgctgaaggt agtgatgcgt ttttgctgtg
     7501 catgtcgcgc aattgaaggc agtgaatcta caatcgctga ccagctttct ccgtcaatgg
     7561 cacgagaaat cgcccatgcg atgactaccg cgcctgcaac cgccagatcg gatttatggg
     7621 tcgggctgga cgccagcgcc acatcatcaa taaaggaatc aacatcgtgc gccggaagca
     7681 aacaacctaa tggcgagacg cgcatcgctg cgccgttggt cacgccgttg ttttccagtt
     7741 cagcaatggg tttaccgtcg cgaatggcgt taagcgcaat cttcgaggtc ggaccaagta
     7801 cgtttttgtt aaaggcgtcg aaacgcagcg cccagtcgag aatattacgc ccaatcagat
     7861 tcggatcgat cttgccttca cgttccagta acgcatccgc cagacacagc gccatcgagg
     7921 tatcgtcggt gaattcggcg cggttaaaat aacaggccgc gttattctcc tttggtccag
     7981 gaagaaaacg gtcaatccag ccaaagtgtg ctttaacgcg gctgcgtggc caaagctcgg
     8041 agggcatccc catcgcatcc cctaacgcct gcccataaag agcaccgaga atacgttctg
     8101 ttttcattta acttcccctt gtgtcaacgc aatatcgcga tcatcgacct tgatagccgt
     8161 aatttcgttg tcggattcgc ggaaaaaaat catgaacagc acggcgataa tggcaatcat
     8221 caccgcgccg aaagtccaca tcccggacca gttgaaagtc agtccgttta ccggttcctg
     8281 ataagcgaac atcttttcca tcatcacacc gccaagacga tagccgagca aactgccgaa
     8341 gccctggcag cagagcgtga tcagcccctg cgcagcggta cgcatatgca cgggggcttt
     8401 tttatcgaca tagatgtaag cggtaacgta gtaaaaatcg taacttacgc cgtgaagcaa
     8461 aataccgagg aacagtaacg cgtaggtgaa atattcatcc gcactaccgt aaataaagaa
     8521 gccatagcgg atcgcagcgg tgaccagacc aagcaataat acctttttga taccaaagcg
     8581 tttagtgaaa aacggcaatg ccagcataaa gaagatttca gagaactggc cgagcgtcat
     8641 ccagccggtg gcgtttttca tgccaacttc ggtcagataa ccgttggcaa agatgtaata
     8701 gaacgctagt ggcatcgcaa acaggaatga acagaagaaa aagacgagga agtttttatc
     8761 gcgcagcagg atcagcgcat ccaggccgag catgacttta atatccattt tgccggtgct
     8821 ttttggtggc gtgtcgggca ggaaaaacgc aaacacaccg agcagagcag aacttccggc
     8881 ggtaatcagc agcgggatgt tagtcggtga gatatcggca taccccagta tttgcggcaa
     8941 gaaaccacat gccagaccgg aggcaatcca gccgatagtg cccatcacac gaatgcgcgg
     9001 gaaatcacgc tcaacatccg gcacgttggc aaaagcgatg ctgttagtca gcgcaatggt
     9061 cggcatatag gttagcgagt aggccagcag taacgggaag aacccggcaa aagtggtctg
     9121 ttgcgcagcg aaatacatca gcagcgcgcc tgcgaacatc aataccgcca gcactttctg
     9181 cgccgagaaa aagcggtcag tgatggagcc aaccagaatt ggcgacagga tcgccgcaat
     9241 ggcggtacag gcatacgacc agccaatttc tccggcacta aaaccgcttt tacttaacca
     9301 caaccacaat ggcacaaacc acgcgcccca gataaaccat tcaacaaaca tcatgaacga
     9361 cagctttgct gttgttttca tcttttaatt ccttcatgac aggtaatgta aggtacgcga
     9421 tgacaatacc acttaataat accttttaaa tacctttgcg gtgaaaactt gaccgcgcta
     9481 acattttttc tgatgaatcg agccaacaga aaacgctgaa aaaacatcca aaagatggaa
     9541 aaactcgccc gtaacgacca ggattatgat tgcccgcaaa aggcgggcca ggaaaatcca
     9601 tagccgatat ccaatcggaa tttacgggag catagtaatg acagatattg cgcagttgct
     9661 tggcaaagac gccgacaacc ttttacagca ccgttgtatg actattcctt ctgaccagct
     9721 ttatctcccc ggacatgact acgtagaccg cgtgatgatt gacaataatc gcccgcctgc
     9781 ggtgttacgt aatatgcaga cgttgtacaa caccgggcgt ctggctggca caggatatct
     9841 ttctattctg ccggttgacc agggcgttga gcactctgcc ggagcttcat ttgctgctaa
     9901 cccgctctac tttgacccga aaaacattgt tgaactggcg atcgaagcgg gctgtaactg
     9961 tgtggcgtca acttacggcg tgctggcgtc ggtatcgcgg cgttatgcgc atcgcattcc
    10021 attcctcgtc aaacttaatc acaacgagac gctaagttac ccgaatacct acgatcaaac
    10081 gctgtatgcc agcgtggagc aggcgttcaa catgggtgcg gttgcggttg gtgcgactat
    10141 ctattttggc tcggaagagt cacgtcgcca gattgaagaa atttctgcgg cttttgaacg
    10201 tgcgcacgag ctgggcatgg tgacagtgct gtgggcctat ttgcgtaact ccgcctttaa
    10261 gaaagatggc gttgattacc atgtttccgc cgacctgact ggtcaggcga accatctggc
    10321 ggcgaccatt ggcgcggaca tcgtcaaaca gaaaatggcg gaaaataacg gcggttataa
    10381 agcgattaat tacggttata ccgacgatcg cgtgtacagc aagttaacca gcgaaaaccc
    10441 gattgatctg gtgcgttatc agttagctaa ctgctatatg ggccgggctg ggttgataaa
    10501 ctccggcggt gctgcgggcg gtgaaactga cctcagtgat gcagtgcgta ctgcggttat
    10561 caacaaacgc gctggcggaa tggggctgat tcttggacgt aaagcgttca agaaatcgat
    10621 ggctgacggc gtgaaactga ttaacgccgt gcaggacgtt tatctcgata gcaaaattac
    10681 tatcgcctga tacgccttat acgaacaaca ccaccccgta ggacggataa ggcgtttacg
    10741 ctgcatccgg caacctatgc ctgatgcgac gctgaagcgt cttatcatgc ctacatagca
    10801 ctgccacgta tgtttacacc gcatccggca taaaaacacg cgcactttgc tacggcttcc
    10861 ctatcgggag gccgtttttt tgcctttcac tcctcgaaca attttcatat tgt
//
LOCUS       DAPHBJ010000149        10871 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.151, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000149 DAPHBJ010000000
VERSION     DAPHBJ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.151"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            52..999
                     /locus_tag="RHZ76_003395"
                     /pseudo
     CDS             52..999
                     /locus_tag="RHZ76_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275144.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
     gene            complement(1135..1770)
                     /locus_tag="RHZ76_003396"
     CDS             complement(1135..1770)
                     /locus_tag="RHZ76_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813985.1"
                     /translation="MSSLIFYTDEAQALIVTDTLAVTDNGEPLSFVSKAGYIPQLRTI
                     IAGTGAGGFSNTWLLEASTRMVVSGIQNLNYHTSACLRRLWKQYKHDHAIPSSSTVTT
                     SVYQFAICEETGNIVSFAYRSVHDFESEQLQYGTGVKPECQILDGDLLQTIPVMMNEQ
                     RAIQATYPHPERIHIGGEMIAHYLTSSMYQCFKIGEFEDFRTQGEYILSRI"
     gene            2384..3631
                     /locus_tag="RHZ76_003397"
     CDS             2384..3631
                     /locus_tag="RHZ76_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV2813986.1"
                     /translation="MRIITNIHARYGRVSKLDATEPKLKFEPGFLYAEIPYISGIQNR
                     KLSRYVHFYLMDKAILQARDLKPLARAFLRWIRWLEDNGLDPFTPGTLKYQSPTYGFR
                     EYLLGAVRQGRIASSTAAVYISVIRRFYVFLGKCGELAASSFFTLVEKVVDGRRKVIS
                     SDLAIRITSTTKRTINPLNDAEVHILDEALKSESGLFSLMIVLMKNCGLRLDEMLTLP
                     SSLFTEEYLASVPVSELTIRGLHIGPRCGVHTKFGMTRELFITRRLYEQVLDYLADEE
                     YEKKIQKWRARQGGEGRHEPLFLMANGKRMSEKAFYSRWYSFRHRLARSAPGNVFRHK
                     PHDLRATFATHFLRSALSCYPDQAANALGTVKYWMGHKSENTTMKYIVFLQQNQISDA
                     VAGVMDALIDGAAGRDGAIYEPE"
     gene            3618..5384
                     /locus_tag="RHZ76_003398"
     CDS             3618..5384
                     /locus_tag="RHZ76_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813987.1"
                     /translation="MSRSETQITWMAENVLSWETVSGHITQRDLNRYYLLDVYLQAHF
                     SDYVLLPGWFSEHSHLARPEKRDFIASLKRFLLSYRPAGRLTIFRYLNMFFIWQSVNQ
                     YDYGTAEGVEMYIKHLMNDIRRRKISVRMARHYLSVVNRFLLTGGQIQRRYEYEFLPK
                     GTYQQQGRDSYTRNELSEILRQLSSMNEFLAGCIREHIAKSEKGVRHFSPSLLAPVYE
                     AYFRYPGENGVSRAEIIIRDVLENYFLTSFFLFCYHTWGNTSQVLGLTRDDIHLDEKG
                     ISTDYVYKGRANKYIRLTIGKSEYVTKRAGYYWFLSFIRLRDDIVNYLVSADNFPPVQ
                     ALFLSEPQVKFRKLYSLNPSHLTKFSNSEGAWATMRQLNPSLPSITVSGLRKTSEQYT
                     DRTLKNGLITAEKAQHNWGTYRRNYAAGNPQGAKENFSAALDTLMNQGIATRALSERV
                     KVADELGIDLRGSDEGVDLLLNGLGCRSQEPPTDIELRFIKKQKRFGRTPKACADFSH
                     CVECSKSCVVETLESVWLLLSFRHAIEYGKPLYIGSVNAVERYETLLLKIDLRLGLVD
                     EATLKKARVKLQREGVAPVWQI"
     gene            5372..7489
                     /locus_tag="RHZ76_003399"
     CDS             5372..7489
                     /locus_tag="RHZ76_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813988.1"
                     /translation="MADLKKKEVSRSRMAEFPAGTPLRSGDEFPTEDIICEALENKSF
                     ERLNNCLVIASSDEHDMRFINEETLNIYFRDDVWTNRYVRTHIGETLRTVNFSGIRDP
                     ELRLEAKIISASLLWIVGRTAKTQSLIRKCANIVAGARIIESMGYRSLFQLRFPLVRN
                     AFIVKMKEEDEHGRKRSERTLRNYFMDLADISLTGHTRLRKYGYFPDISFDSYNSVDD
                     SNQTYCMPFSLMMSVWEGLITELDAEISDFDFDGFSRLCAIINGFYDSPYYTSMLEAR
                     KRGTAKSIPDYRAAYYYNIAAGEIRALFATLGPGMQGWFSVHKNKRWRSDFIGVDHIK
                     LNTWHFELTLKVMNVIQAMSGMRHSEVLGVMHGSLIYDGDILGLRSVLHKFAPEGGSH
                     EDWVVCRYVEKPFQHLRRINQIMTGLDGKTLDNVPLSLNIRSWFSEQKLSFMGTQRQT
                     EWAKKFVKRHHLFIRRDHIDEFRLLNPNIRDDERVAMEIREGAFWPLRTHQFRRSLAV
                     HLRRLDLISTNDLIRQFKHLIRGMTEWYMSGALNASHFSRAIPQAFAAEIERVDTELS
                     ASRAVSYQHEGLLYGEGGKLLMSQRGGHLHQMTFPTLKKAMSMAKRGGQKLVSLGNGF
                     YCMNGHDCEFKAVVQSASCNPGCENMLAGADSVPVWKRRMAHYDNLLEIAVRNNAPQA
                     DRDFLMLERDFYADAVRFFEKDE"
     gene            7493..7921
                     /locus_tag="RHZ76_003400"
     CDS             7493..7921
                     /locus_tag="RHZ76_003400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2813989.1"
                     /translation="MTNEQSGVKKTQARAEQALRELMLSGERISQYAVEKRAGLANGT
                     LNYNCPEYRQVREAIRSLKKTCQGTAPVDEQGIEQQIKLKEKYRRQRNELREEIKKMM
                     AENVELLHNLILLQQYVRELERNTGREKGDVIPFSGGVRD"
     gene            8006..8676
                     /locus_tag="RHZ76_003401"
                     /pseudo
     CDS             8006..8676
                     /locus_tag="RHZ76_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558297.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(9247..9555)
                     /locus_tag="RHZ76_003402"
     CDS             complement(9247..9555)
                     /locus_tag="RHZ76_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003256877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2813990.1"
                     /translation="MPDIDLNDVIKALGNPVRRKILYMLKEPKIHFHQPYADADEFGV
                     CVSIIQEKVGLSQSTVSLYLSALQRAQLVTSQRIGPWTYYKRDNDKIRVVLELLKNTI
                     "
     gene            complement(9651..10775)
                     /locus_tag="RHZ76_003403"
     CDS             complement(9651..10775)
                     /locus_tag="RHZ76_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HDV2813991.1"
                     /translation="MKTKNLFSPTRLGPYTLKNRVVLPPLTRSRSSQPGDIPNELMAE
                     YYAQRSSAGFMVTEGTQIEPRGQGYAWTPGIYSSRQVEGWKKVTRAVHEKEGIIFAQL
                     WHVGRVSHNSLQPGNASPVAPSAIPAGENVKVFIETGPGEGALATASCPRALEKEEIS
                     EIVRMYAGAAQNALDAGFDGVEIHCANGYLVNQFMSSHSNKREDEYGGSLHNRLRFLR
                     EVTQAVADVVGKDRVGVRFAPLFQTTDEVRVYLGLVEDDPHETYTEAVKILEEIGIAY
                     ISLAEADWDDAPELPEAFRAEVRKLFSGVIMYAGRYTQEKAERIIGAGWGDIIGFGRP
                     FIANPDLPQRLYHEWPLNPVDPTTMYGGTAKGYTDYPAFG"
BASE COUNT         2769 a         2452 c         2780 g         2870 t
ORIGIN      
        1 gatttaataa atatcgcgac aatacagcga aagccaaatt caggaaataa tatgtatatc
       61 gaaatgaaag aaacattcct tgaattaccc tcagaaattc aattttctga ggcggctaac
      121 gctgccattc tgacgcagga taaatggggc gatgtctggc agacactgaa gaccgatgcg
      181 gatctgaatt atactgatgc cggtgagccg gtcagtctcg cttcgcgcgt gacgacggca
      241 accagtgcca tttatcatgc gattaccgaa ggctggacca tgtgcgtcgg ctacagcggc
      301 gggaaagact ctcactcgct actgcacttg ttccttatgg ccttgatccg tgcagtacga
      361 aacgggtcga acgtcagcca gcaccacttc attcagatgt ctgacaccct gattgaaaac
      421 cctgagatgc atcatcaggc cacgcaggtt ctcagccagc tcagagcctt tattgccgaa
      481 caagaactgc ctttgaccgt ccttgtggcc cgcccgtcca tgacccagag ctgggtcgga
      541 cgcattctga ccgggcgtgg acttccaacc tggacaaatt cttctacgcg tcaatgcgct
      601 acagatctaa agcgttctcc actgcgccag gccaaagccc gttatctgaa aaatgcacct
      661 gcttctgtcc gcaacaaagt gtgcctgatg ctgggttccc gcgatgatga gagcgcccgt
      721 cgggcagaca acatcctaag aatgggcggg caggcggaca gcgtctcgct cacggagcac
      781 ggcggcgaac tgtacccggt gaaagagtgg cgggcacagg atatctggtc atttctgatg
      841 gcctgtggtt ctctggcgcg atttccgctg ccaagctttt tgccggacaa cttcagcctc
      901 gccacgcttt acaaagacgc gaccggggaa tgtatctgga cgccggagaa atccactgtc
      961 cggtcttctg cctgcggcgc tcgctatgga tgctcgctta ctcttgtttt tatgtattta
     1021 gagttaaact ctacttttct atttttagaa ttcaagctat tcatcgtaat tgagagatat
     1081 ccttttaatg ttcagcaagc ctgttaaaag cactttatct gaaccgcgtt ttgcctaaat
     1141 cctgctcaat atatactctc cctgagttct gaaatcctca aattccccaa tcttaaagca
     1201 ttgatacata gatgatgtca gataatgcgc aatcatctca ccgccaatat ggatccgttc
     1261 cgggtgtggg tatgtcgcct gtatagccct ttgttcattc atcattacag gaattgtctg
     1321 caacaaatcc ccatccaaga tttggcactc aggttttact cctgttccgt attgtaattg
     1381 ctctgattcg aaatcatgga ctgagcgata ggcgaacgaa acgatatttc ctgtttcctc
     1441 gcaaattgcg aactgataaa cactggttgt caccgttgaa gaagagggaa ttgcatggtc
     1501 atgcttgtat tgtttccaca gcctccttag acaggccgat gtatgataat tgagattttg
     1561 tatacctgat acgaccattc tggttgatgc ttcaagcaac caggtgttag aaaatccacc
     1621 tgcccccgtt cctgctatga tggttcttag ctgggggatg taacctgctt tggaaacaaa
     1681 agataacggc tccccattat cggtgacggc aagggtatct gttacaatca gtgcttgtgc
     1741 ttcgtctgta taaaatatca gtgatgacat attttcctca tccagaaaag acattacatg
     1801 aatcacagaa caggtcacta caaccacgtg aaagggagtc agaccatttt ccagaggtaa
     1861 atatattctt gtgtttggac gcgttgcggt tctctgtaaa aaatatatag cacaaacaaa
     1921 agtccccgca gggataaata tagttatatg cgctttttct gctggctttt acaattgaaa
     1981 gtgacaggta aacgaaatgt gttacaggtt tcttttgctt ttcgttgggt tctgtatatg
     2041 cgaatttacc ctgtgtgtta tttttgcaat ggactcaggc tgtattcaat caattaaggt
     2101 ttacttctgg cattaagatg tatccgttgc ctgtttttta agggcacggt ttgtgccggg
     2161 aaatgaaaat cacatcttaa gttcttcact caaaggattt gtgatgttta tcacgtatgg
     2221 tccgggtaac gtgttgaggt caatgtattg catcgtgatg gtgtcgttac atcttttact
     2281 ttcctcctga tttgcatatt cactcccggc aacctggttg tttgtttttt cttcttccgg
     2341 atttatagtg taggcatgag gtggcgtttc agggcgacag actgtgagga taatcactaa
     2401 cattcatgcg cgttatgggc gtgtcagcaa actggatgca acagagccga aactgaaatt
     2461 tgaacccgga tttctgtatg ctgaaatacc ttatattagc ggcatccaga atcgaaaact
     2521 gagccgatat gtacactttt atcttatgga taaagctatc ctgcaggcca gggatttaaa
     2581 gcctttagcc cgggcatttt tacgctggat aaggtggctg gaggataacg gacttgaccc
     2641 tttcaccccg ggaacgttga aataccaaag ccctacatat ggcttcaggg agtatcttct
     2701 gggggccgta aggcaggggc gcatcgcctc atccaccgcg gcggtgtaca tcagcgttat
     2761 ccgacggttt tacgtatttc tgggcaagtg tggtgagctg gctgccagct cattttttac
     2821 gttggtcgaa aaagtcgttg acgggcgacg caaggtgata agttcggatc ttgccatcag
     2881 gataaccagc acaacaaaaa ggacaatcaa cccactgaat gatgcagagg ttcatatcct
     2941 ggatgaggca ctgaaatctg agtccgggtt gttcagttta atgattgtgc tgatgaaaaa
     3001 ttgtgggcta cggctggatg aaatgctgac cctgccgtcg tcactcttta ctgaagagta
     3061 ccttgccagt gtccctgtat ctgagttgac catcaggggg cttcatattg gtccgagatg
     3121 cggagttcat acgaagtttg ggatgacgcg agaactcttc atcaccagac gattgtacga
     3181 gcaggttctg gattatctgg cggatgagga gtatgaaaag aagatccaaa aatggcgtgc
     3241 acgacagggt ggggaagggc ggcacgagcc tttattttta atggcaaacg ggaaacggat
     3301 gtctgaaaaa gccttttact cccgctggta cagttttcgc catcgcctgg cccggtctgc
     3361 tccgggaaat gtgttcagac ataaacccca tgatttgcgg gcaacgtttg cgacccattt
     3421 tttacgctcg gcactgagtt gctatcccga tcaggccgca aatgcgctgg gtacagtaaa
     3481 atactggatg ggccataaaa gcgaaaatac gacgatgaaa tacatcgtct ttcttcagca
     3541 aaaccagatt tctgatgcag tcgccggggt catggacgca ttgattgacg gtgctgcagg
     3601 tcgcgatgga gccatatatg agccggagtg aaacgcagat aacgtggatg gccgaaaatg
     3661 tgctgtcatg ggagacggtc agcggacaca tcactcagcg ggatctgaac cgatattatt
     3721 tacttgacgt ttatcttcag gctcattttt cagattatgt gttgctcccc ggatggttta
     3781 gtgaacattc gcatcttgcc cgccctgaaa aacgcgattt tattgcctct ctgaaacgat
     3841 ttcttctgtc ttaccgtccc gccggaaggt taaccatttt ccgttatctc aatatgtttt
     3901 ttatatggca gtcagtcaat cagtatgact atggcacggc tgaaggcgtt gaaatgtata
     3961 ttaaacatct catgaacgat attcgtcggc gaaaaatttc agtcaggatg gcaagacact
     4021 acttgtccgt ggtcaaccgg tttttgctta cgggcgggca gattcagagg cggtatgaat
     4081 atgaattctt accgaaagga acgtatcagc agcagggaag ggatagctat accaggaatg
     4141 aactttccga gatcctgagg cagttgtcat ccatgaatga gttccttgcg ggatgtatcc
     4201 gggagcatat cgctaaatcg gaaaaagggg tgcggcattt ctcaccgtct ttgctggcac
     4261 ctgtttatga agcgtatttc cggtatccgg gagagaacgg ggtcagcagg gcagagatca
     4321 tcatcaggga cgttctggaa aattactttc ttacttcttt ttttctgttc tgttaccaca
     4381 catggggaaa cacctctcag gttctgggtc tgacgcggga tgatatacat ctggatgaaa
     4441 agggtatcag cactgattat gtttataagg ggagagcgaa taagtatata cgtctgacga
     4501 taggtaaatc ggagtatgtg accaaacgtg cgggctatta ctggtttctg tcttttatca
     4561 ggctcaggga cgacatcgtt aactatctgg tttccgcaga taacttccct ccggtacagg
     4621 cgctgtttct gagtgagccg caggtgaaat tccgtaagct ttattctctt aatcccagtc
     4681 atctgactaa attttctaac tctgaggggg cgtgggccac gatgcggcag cttaatccct
     4741 cgcttccatc aattaccgtt tctggcttgc gcaaaacgtc tgagcagtat acggacagaa
     4801 cattaaagaa tggtctgatt acggcggaaa aagcacagca taactggggc acttacaggc
     4861 ggaattatgc tgcaggtaac ccacaggggg cgaaggaaaa cttcagtgct gcacttgata
     4921 cgcttatgaa tcaggggatt gcgacacgtg cgttatccga gcgggtaaaa gttgctgatg
     4981 agctggggat cgatctgcgt ggaagcgatg agggtgtgga tcttttattg aatggcctgg
     5041 gatgtcgatc gcaggagccg cctacagata ttgaattgcg ctttataaaa aagcaaaaac
     5101 gatttggccg gacaccaaag gcttgtgcgg atttttctca ttgtgtcgaa tgttcaaaaa
     5161 gttgcgttgt ggaaacgctg gagtctgtat ggctcctgct gtcattcagg catgctatcg
     5221 agtatggaaa accgttgtac atcggttcgg tcaatgctgt ggagcgttat gaaacgttac
     5281 tcctgaagat tgatttacgg cttgggctgg tagatgaggc gaccctgaaa aaagcgcgcg
     5341 ttaaacttca gcgagaaggt gtggctccgg tatggcagat ttgaagaaaa aagaggtaag
     5401 ccgtagtcga atggcggagt ttcctgccgg aacgccgctt cgttcagggg atgagtttcc
     5461 gactgaggat atcatctgtg aggcactaga aaacaaaagc tttgaacgtc tgaataactg
     5521 cctggttatc gccagctcag atgaacatga tatgcgtttt atcaatgagg agacgctgaa
     5581 tatttatttc agggatgatg tctggaccaa tcgctatgtc agaacccata ttggtgaaac
     5641 gttaagaacc gttaattttt caggcatacg ggatcctgaa ctgcgtcttg aagccaaaat
     5701 catctcagcc agcctgctct ggattgtcgg caggaccgcg aagacgcagt cactgatccg
     5761 gaaatgtgca aacattgtgg cgggagcccg tattattgag tctatggggt atcgcagtct
     5821 gtttcagctt cgttttccgc ttgtccggaa tgctttcatt gtaaagatga aggaggagga
     5881 tgagcacggc aggaagcgtt ctgaaagaac attacgtaat tactttatgg acctggcgga
     5941 tatctcgctg acaggtcata cccgtcttcg aaaatacggt tactttcctg atattagctt
     6001 cgacagttac aacagtgtgg atgattcaaa ccagacatat tgtatgcctt tcagtcttat
     6061 gatgtctgtc tgggaagggc tgattactga actggatgct gaaatcagtg atttcgattt
     6121 tgacgggttt tcccggctgt gcgctatcat aaacggtttt tatgattcgc cttactatac
     6181 gtcgatgctg gaagcccgta aaagagggac agcaaaaagc ataccggatt accgggccgc
     6241 gtattactac aatattgctg ccggggaaat tcgggcgctt ttcgcgacac ttggccccgg
     6301 gatgcagggc tggttctcgg tacataaaaa taagcgctgg agatctgatt ttattggcgt
     6361 tgatcacata aagctgaaca cctggcattt tgagctgacg ttaaaagtca tgaatgtgat
     6421 acaggccatg tcggggatga gacacagcga agtgcttggc gtgatgcacg gttcactgat
     6481 ttatgatggt gatatcctgg gacttcgttc ggtgttgcat aagttcgccc cggaaggtgg
     6541 aagtcacgaa gactgggttg tttgtcgcta tgtggaaaag ccttttcagc atttacgcag
     6601 aattaatcaa atcatgacag gcctggacgg aaagacgctg gacaatgttc cattgtcttt
     6661 aaatatcagg tcgtggttct cggagcaaaa actctctttt atggggacgc agcgccagac
     6721 tgagtgggca aagaaatttg tgaaaagaca ccatctgttc atccgacgtg accatattga
     6781 tgagttcagg cttttaaatc caaacatacg tgatgatgaa cgcgttgcga tggagattcg
     6841 ggagggggct ttctggccat tgcgaaccca tcagtttcgc cgttcacttg ctgtacacct
     6901 caggcgtctt gatctgattt ctactaatga tcttatccgg cagtttaagc acttaatcag
     6961 aggcatgacg gagtggtata tgtccggtgc actcaacgct tcgcatttta gccgggcgat
     7021 ccctcaggca ttcgctgccg agattgaaag ggttgatacc gaactctcag cttccagggc
     7081 tgtttcttat cagcatgaag gcctgcttta tggcgaaggc gggaagttac tgatgtccca
     7141 gagaggtggg cacttacatc agatgacgtt tcctacactg aaaaaagcga tgagcatggc
     7201 taaacgaggc gggcaaaaac tggtatctct tggaaacggt ttttattgca tgaatggcca
     7261 tgactgcgag tttaaggcgg ttgttcagtc agcgtcctgc aatccgggat gtgagaacat
     7321 gctggccgga gctgattctg ttcccgtgtg gaaacgcagg atggctcatt atgacaacct
     7381 tctggagatt gccgtgcgaa ataatgctcc tcaggctgac agggattttc tgatgcttga
     7441 gagagatttt tatgcagatg ctgtccgctt ttttgaaaag gatgaataga gcatgacgaa
     7501 cgaacagtcg ggggttaaaa aaacacaggc aagagcagag caggccctga gggaactgat
     7561 gttatcaggg gagcgtatca gccagtatgc tgtggaaaaa cgagcggggc tggcaaacgg
     7621 caccctgaat tataactgtc ctgagtacag gcaggtcagg gaggctatcc gcagtctgaa
     7681 aaaaacgtgc cagggcaccg cgccggttga tgagcagggc attgagcagc agatcaagct
     7741 gaaagaaaag tatcgccgtc agagaaatga gctgagggaa gaaatcaaaa agatgatggc
     7801 tgaaaatgta gagctgttac ataaccttat acttttacag cagtatgtca gggagcttga
     7861 acgcaacacc gggagggaga aaggggacgt cattccgttc tcgggtggtg tcagggactg
     7921 aggctatact gaacggaagg acgcgaggtg aatcgccacg ggtttaacag acacctcaga
     7981 gtcatttaag atggcttaaa gagaggtgcc catgagcggt aagcgttatc ccgaagagtt
     8041 taaaattgaa gcagtcaaac aggttgttga tcgcggttat tctgttgcca gcgttgcaac
     8101 gcgtctcgat atcaccaccc acagccttta cgcctggata aagaactacg gtccggattc
     8161 ttccactaat aaagaacagt cagatgctca ggccgagatc cgccgtcttc agaaagagct
     8221 gaagcgggtt accgacgaac gggacatatt aaaaaaagcc gcggcgtact tcgcaaagct
     8281 gtccgactga ggtacgcctt tattcgtgac aacacctgtt gctggcctgt tcgcctgctc
     8341 tgtcgggtgc tggatgaaca tcccagtggt ttttacgcct ggcttcagca gccgcattca
     8401 caacgccatc aggcagacct gagactgaca ggacagatta aacagttctg gctggaatcg
     8461 ggatgcgtct atggttatcg caaaatccat ctggatctgc gggacagcgg gcaacagtgc
     8521 ggagtgaaca gagtctggcg actgatgaaa cgtgtcggga taaaggctca ggtcggatac
     8581 cggagcccga gggcatgtaa aggcgaggcc agtatcgtgt cgcccaacaa gctccagcga
     8641 cagttcaatc cggatgctcc ggatgagcgt tgggtaatca cagcgtggtt tcctcaaacc
     8701 ctcgctgtga attaactgaa atacttcatt gaagctgtaa ccaaacagtt aactgaaaca
     8761 atgagcccgc agagcattgc acagcgatga caccatttgt tgagcttcac cgttttcgca
     8821 tacaaccgtc cgggatttgg aaaagcactg tctttttgta ctgaccgatc atgattaagt
     8881 gacacctctt cgaaaaagag agcgcgatga gcgattaata tatggctcga aagacaatta
     8941 tgttgggctt ttatgaccgc tgtcagaggt attatcttac ccggacggca acagggtcgg
     9001 aatgtcatca gcggcactgt aacaagctat ccgccatctt aaatatctgc aggtcctttc
     9061 accgagtatg cacaccaacc gacagagtga tttgacccaa actggtggat attttgcaaa
     9121 actattagcc tcttatgacc ggtctgcagc ctgatattga ggaattagtg cgctttcatg
     9181 gaatgcctgc cgggatcgca agtacgaacc cccggtactg ttacactgaa tgtgctcagt
     9241 cctgtatcag attgtatttt tcagcaactc aagaacaacc cttattttat cattgtcacg
     9301 tttgtagtac gtccagggac cgatgcgctg agaagtaaca agttgtgccc gttgaagagc
     9361 tgagaggtaa agagaaacgg tagactgtga gagcccgacc ttttcctgaa tgatactgac
     9421 acacacaccg aactcgtccg catcagcata aggctgatgg aagtgtattt tcggttcctt
     9481 gagcatatac agaatttttc ttctaacagg gtttcccagc gccttaatca cgtcattcaa
     9541 atcaatatca ggcataaact ctgtcacttg tcgcattgag gaattgcaat gttacgatac
     9601 ttctctacgt taagcatcag cttttgaaaa gctgatgctc ataagaatgg ttagccaaag
     9661 gcgggataat ctgtgtagcc ttttgccgtt ccaccataca tggttgttgg atcgaccgga
     9721 ttcagtggcc attcatgata aaggcgctgc ggtaaatccg gattagctat gaacgggcgg
     9781 ccaaatccga taatgtctcc ccagccagca ccgataatcc gttcggcttt ttcttgcgta
     9841 tatcgcccgg catacataat tacaccgctg aataatttac ggacttcagc tctgaatgct
     9901 tcaggcaatt cgggagcatc atcccagtcg gcttcagcaa gagaaatata ggctattccg
     9961 atttcttcaa gaattttaac ggcttcggta taggtttcat ggggatcatc ttccaccagg
    10021 ccaagataga cccttacttc atccgtggtc tgaaagagcg gagcaaaacg gactcccaca
    10081 cggtctttac cgaccacatc agcgacagcc tgcgtcactt cgcgcagaaa ccgcagccgg
    10141 ttatgtaatg agccaccata ttcatcctct cttttgttgc tatgggatga catgaactgg
    10201 ttaaccaggt atccatttgc acagtgaatt tccacaccgt caaaaccggc atcaagggcg
    10261 ttctgagctg ccccggcgta catcctgaca atctcactaa tttcctcttt ttccagtgcg
    10321 cgggggcagg aagcggtagc cagcgccccc tctcccggcc cggtttcgat gaatacctta
    10381 acgttttccc ctgccggtat cgccgacgga gcgacaggcg aggcgttacc tggctgcagg
    10441 gagttatggg atactcttcc gacatgccag agctgggcaa aaatgatgcc ttctttttca
    10501 tgcactgccc gcgtcacttt cttccagcct tccacttgcc gtgagctgta gatccccggg
    10561 gtccacgcat aaccctgccc acgaggttcg atttgtgttc cttcggtcac cataaacccg
    10621 gcactgctgc gctgtgcgta atattcagcc atcagttcgt tcggaatatc acccggttgt
    10681 gagctccgtg agcgggttaa aggcggaagt acaacacgat tcttcagggt gtagggacct
    10741 agtctggtgg gagagaacag gtttttcgtc ttcattacat atcctgatat agcgaatttt
    10801 caatatcact gaaacgccat gtctcagcat ttacgacctg gcatgtcgtc gttcggccga
    10861 tgtagccgca g
//
LOCUS       DAPHBJ010000150        10781 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.152, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000150 DAPHBJ010000000
VERSION     DAPHBJ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.152"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            13..1155
                     /locus_tag="RHZ76_003404"
     CDS             13..1155
                     /locus_tag="RHZ76_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV2813992.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGKDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESRLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1162..1926)
                     /gene="glcC"
                     /locus_tag="RHZ76_003405"
     CDS             complement(1162..1926)
                     /gene="glcC"
                     /locus_tag="RHZ76_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HDV2813993.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2177..3676
                     /gene="glcD"
                     /locus_tag="RHZ76_003406"
     CDS             2177..3676
                     /gene="glcD"
                     /locus_tag="RHZ76_003406"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HDV2813994.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3676..4728
                     /gene="glcE"
                     /locus_tag="RHZ76_003407"
     CDS             3676..4728
                     /gene="glcE"
                     /locus_tag="RHZ76_003407"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HDV2813995.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARVGTPLVTIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMVGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4739..5962
                     /gene="glcF"
                     /locus_tag="RHZ76_003408"
     CDS             4739..5962
                     /gene="glcF"
                     /locus_tag="RHZ76_003408"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HDV2813996.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERILREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            5967..6371
                     /locus_tag="RHZ76_003409"
     CDS             5967..6371
                     /locus_tag="RHZ76_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HDV2813997.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6393..8564
                     /gene="glcB"
                     /locus_tag="RHZ76_003410"
     CDS             6393..8564
                     /gene="glcB"
                     /locus_tag="RHZ76_003410"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HDV2813998.1"
                     /translation="MSQTITQSRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8919..10601
                     /gene="glcA"
                     /locus_tag="RHZ76_003411"
     CDS             8919..10601
                     /gene="glcA"
                     /locus_tag="RHZ76_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HDV2813999.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT         2521 a         2725 c         3023 g         2512 t
ORIGIN      
        1 cagaacagaa ctatggaatg cgatctgctg atgattaaaa ttgaaaaagt aattaataaa
       61 aatgacctta aggcatttat cgcttttccg tcatcacttt atcccgacga tccaaactgg
      121 ataccccctt tattcattga acgcaacgag catttgtctg cgaaaaatcc ggggacggac
      181 catatcatct ggcaggcgtg ggtggcaaaa aaagcggggc agatagttgg gcgtattacc
      241 gcgcaaatag ataccttgca ccgcgagcgt tacggcaaag ataccggtca tttcggcatg
      301 attgacgcca ttgatgatcc gcaggttttt gccgcgttgt ttggcgcagc ggaagcgtgg
      361 ttgaagtcac aaggtgcaag taagatcagc gggcctttca gcctgaatat caatcaggaa
      421 agcagattac tgattgaagg ttttgacaca ccaccctgtg cgatgatgcc acacggcaaa
      481 ccgtggtatg ccgcgcatat tgaacaactg ggttatcaca aaggcattga tttactggcg
      541 tggtggatgc agcgaaccga tctcactttc tctccggcgc taaaaaaact gatggatcag
      601 gtgcgcaaaa aggtgaccat tcgctgcatc aatcgtcagc ggtttgccga agagatgcag
      661 atcctgcgtg agattttcaa ctctggctgg cagcacaact ggggatttgt gccgtttacc
      721 gaacatgaat tcgcgaccat gggcgatcaa cttaaatatc tggtgccgga cgatatgatc
      781 tatatcgctg agattgattc tgcaccctgc gcgtttattg tcggcttgcc gaacatcaac
      841 gaggcgattg ccgatctgaa cggatcgctc tttcccttcg gctgggcaaa attgctgtgg
      901 cgtttgaaag tcagcggtgt gcgaactgcg cgagtaccgc tgatgggcgt gcgcgacgag
      961 tatcagttca gccgcatcgg cccggtgatt gcgctgttat tgattgaagc cttacgcgat
     1021 ccgtttgccc gccggaagat cgatgcgctg gagatgtcat ggatcctcga aaccaataca
     1081 ggcatgaata acatgctgga acgcattggg gcggagccgt acaagcgtta ccgattgtat
     1141 gaaaagcaga tttaatacca gctaactcag gttcatctcc agcggcaccg ccgagcgaat
     1201 caaatggtgg ccttccagct cgatatcgtg gagattcttt ttcacggtcc gcacatgatc
     1261 gcgtgctgcg cgctgggcga cgtgcggcag ccgctgcaac accgcgttgt agatccgcgc
     1321 atgctggcga tcgatctgct ttttttgctg tggtcgatgg tagagattat ttacgctggc
     1381 aaacactgaa ttaaacatca gatcggtcaa tgattgcagc gtaaacacca gcacctgatt
     1441 gtgagaagcc tgacaaatgg cgagatggaa agcgtgatcc aactgcgcat gttcgatcag
     1501 cgaaatctct ttgttgttct cactggcggc gagcattttt tcataacagc gggttatcac
     1561 aacaaaatca gcctgcgttc ccagcgttgc cgccagcctt gccgattcgc cctccagtaa
     1621 tgcgcgaacg tcgagcagat cgtacagcgt tcgcggctgc gtactgaaca gatggatcag
     1681 cgggctggtg tcctgcaccc gattaagccg tgcgacacga gaatcgcgac cctgcgccgt
     1741 ttcaataatc ccgcgcccgc gcagcacggt cagcccttca cgcagtgcgg agcgtgagaa
     1801 gccgagcttt tcacacagtc gacgttccga gggaagcggc tgaccgacct tcagtacgcc
     1861 gtcgataatt aaccgttcga tactctctgc aaccacttcg caaatagggc gacgttcatc
     1921 tttcattccc ggacctcgtg cacaggtagg accaattttt ctgttcttaa ctcgcaaaac
     1981 acgcacatca cgtaagtgat tatgttacat caatttaaca ttgagttaac caagacaagg
     2041 tcacagagct ggaaaaaaaa tggtctgacc ggtagctaaa gagatagacg aaaacgaaaa
     2101 gcccgcttaa taactgttca cagaagcagc gcgcaaaaat cagctgccac acaacacaac
     2161 aaagcgaagc ctactcatga gcatcttgta cgaagagcgt cttgatggcg ctttacccga
     2221 tgtcgaccgc acatcggtac tgatggcact gcgtgagcat gtccctggac ttgagatcct
     2281 gcataccgat gaggagatca ttccttacga gtgtgacggg ttgagcgcgt atcgcacgcg
     2341 tccattactg gttgttctgc ctaagcaaat ggaacaggtg acagcgattc tggctgtctg
     2401 ccatcgcctg cgtgtaccgg tggtgacccg tggtgcaggc accgggcttt ctggtggcgc
     2461 gctgccgctg gaaaaaggtg tgttgttggt gatggcgcgc tttaaagaga tcctcgacat
     2521 taaccccgtt ggtcgccgcg cgcgcgtgca gccaggcgtg cgtaacctgg cgatctccca
     2581 ggccgttgca ccgcataatc tctactacgc accggaccct tcctcacaaa tcgcctgttc
     2641 cattggcggc aatgtggctg aaaatgccgg cggcgtccac tgcctgaaat atggtctgac
     2701 cgtacataac ctgctgaaaa ttgaagtgca aacgctggac ggcgaggcac tgacgctggg
     2761 atcggacgcg ctggattcac ctggttttga cctgctggcg ctgttcaccg gatcggaagg
     2821 tatgctcggc gtgaccaccg aagtgacggt aaaactgctg ccgaagccgc ccgtggcgcg
     2881 ggttctgtta gccagctttg actcggtaga aaaagccgga cttgcggttg gtgacatcat
     2941 cgccaatggc attatccccg gcgggctgga gatgatggat aacctgtcga tccgcgcggc
     3001 ggaagatttt attcatgccg gttatcccgt cgacgccgaa gcgattttgt tatgcgagct
     3061 ggacggcgtg gagtctgacg tacaggaaga ctgcgagcgg gttaacgaca tcttgttgaa
     3121 agcgggcgcg actgacgtcc gtctggcaca ggacgaagca gagcgcgtac gtttctgggc
     3181 cggtcgcaaa aatgcgttcc cggcggtagg acgtatctcc ccggattact actgcatgga
     3241 tggcaccatc ccgcgtcgcg ccctgcctgg cgtactggaa ggcattgccc gtttatcgca
     3301 gcaatatgat ttacgtgttg ccaacgtctt tcatgccgga gatggcaaca tgcacccgtt
     3361 aatccttttc gatgccaacg aacccggtga atttgcccgc gcggaagagc tgggcgggaa
     3421 gatcctcgaa ctctgcgttg aagttggcgg cagcatcagt ggcgaacatg gcatcgggcg
     3481 agaaaaaatc aatcaaatgt gcgcccagtt caacagcgat gaaatcacga ccttccatgc
     3541 ggtcaaggcg gcgtttgacc ccgatggttt gctgaaccct gggaaaaaca ttcccacgct
     3601 acaccgctgt gctgaatttg gtgccatgca tgtgcatcac ggtcatttac ctttccctga
     3661 actggagcgt ttctgatgct acgcgagtgt gattacagcc aggcgctgct ggagcaggtg
     3721 aatcaggcga ttagcgataa aacgccgctg gtgattcagg gcagcaatag caaagccttt
     3781 ttaggtcgcc ctgtcaccgg gcaaacgctg gatgttcgtt gtcatcgcgg cattgttaat
     3841 tacgacccga ccgagctggt gataaccgcg cgtgtcggaa cgccgctggt gacaattgaa
     3901 gcggcgctgg aaagcgcggg gcaaatgctc ccctgtgagc cgccgcatta tggtgaagaa
     3961 gccacctggg gcgggatggt cgcctgcggg ctggcggggc cgcgtcgccc gtggagcggt
     4021 tcggtccgcg attttgtcct cggcacgcgc atcattaccg gcgctggaaa acatctgcgt
     4081 tttggtggcg aagtgatgaa aaacgttgcc ggatacgatc tctcacggtt aatggtcgga
     4141 agctacggtt gtcttggcgt gctcactgaa atctcaatga aagtgttacc gcgaccgcgc
     4201 gcctccctga gcctgcgtcg ggaaatcagc ctgcaagaag ccatgagtga aatcgccgag
     4261 tggcaactcc agccattacc cattagtggc ttatgttact tcgacaatgc gttgtggatc
     4321 cgccttgagg gcggcgaagg atcggtaaaa gcagcgcgtg aactgctggg tggcgaagag
     4381 gttgccggtc agttctggca gcaattgcgt gaacaacaac tgccgttctt ctcgttacca
     4441 ggtaccttat ggcgcatttc attacccagt gatgcgccga tgatggattt acccggcgag
     4501 caactgatcg actggggcgg ggcgttacgc tggctgaaat cgacagccga ggacaatcaa
     4561 atccatcgca tcgcccgcaa cgctggcggt catgcgaccc gctttagtgc cggagatggt
     4621 ggctttgccc cgctatcggc tcctttattc cgctatcacc agcagcttaa acagcagctc
     4681 gacccttgcg gcgtgtttaa ccccggtcgc atgtacgcgg aactttgagg agcaggctat
     4741 gcaaacccaa ttaactgaag agatgcggca gaacgcgcgc gcgctggaag ccgacagcat
     4801 cctgcgcgcc tgtgttcact gcggattttg taccgcaacc tgcccaacct atcagcttct
     4861 gggcgatgaa ctggacgggc cgcgcgggcg catctatctg attaaacagg tgctggaagg
     4921 caacgaagtc acgcttaaaa cacaggagca tctcgatcgc tgcctcactt gccgtaattg
     4981 tgaaaccacc tgtccttctg gtgtgcgcta tcacaatttg ctggatatcg ggcgtgatat
     5041 tgtcgagcag aaagtgaaac gcccactgcc ggagcgaata ctgcgcgaag gattgcgcca
     5101 ggtagtgccg cgtccggcgg tcttccgtgc gctgacgcag gtagggctgg tgctgcgacc
     5161 gtttttaccg gaacaggtca gagcaaaact gcctgctgaa acggtgaaag ctaaaccgcg
     5221 tccgccgctg cgccataagc gtcgggtttt aatgttggaa ggctgcgccc agcctacgct
     5281 ttcgcccaac accaacgcgg caactgcgcg agtgctggat cgtctgggga tcagcgtcat
     5341 gccagctaac gaagcaggct gttgtggcgc ggtggactat catcttaatg cgcaggagaa
     5401 agggctggca cgggcgcgca ataatattga tgcctggtgg cccgcgattg aagcaggtgc
     5461 cgaggcaatt ttgcaaaccg ccagcggctg cggcgcgttt gtcaaagagt atgggcagat
     5521 gctgaaaaac gatgcgttat atgccgataa agcgcgtcag gtcagtgaac tggcggtcga
     5581 tttagtcgaa cttctgcgcg aggaaccgct ggaaaaactg gcaattcgcg gcgataaaaa
     5641 gctggccttc cactgtccgt gtaccctaca acatgcgcaa aagctgaacg gcgaagtgga
     5701 aaaagtgttg cttcgtcttg gatttacctt aacggacgtt cccgacagcc atctgtgctg
     5761 cggttcagcg ggaacatatg cgttaacgca tcccgatctg gcacgccagc tgcgggataa
     5821 caaaatgaat gcgctggaaa gcggcaaacc ggaaatgatc gtcaccgcca acattggttg
     5881 ccagacgcat ctggcgagcg ccggtcgtac ctctgtgcgt cactggattg aaattgtaga
     5941 acaagccctt gaaaaggaat aacaaaatga aaactaaagt cattcttagc cagcaaatgg
     6001 cgagtgcaat tattgccgca ggtcaggaag aggcgcagaa aaataactgg tctgtttcca
     6061 ttgctgttgc cgatgacggc ggtcatctgc tggcgttaag tcgcatggac gattgcgcgc
     6121 cgattgcggc ttatatctcc caggagaaag cgcgtaccgc cgcgctgggg cgtcgtgaaa
     6181 ctaagggcta tgaagagatg gtgaacaacg gacgtaccgc gttcgtgact gcgccgttat
     6241 taacgtcgct ggaaggcggc gtaccggttg ttgtggatgg gcaaattatt ggtgccgtgg
     6301 gcgtttctgg tttaaccgga gcacaggatg cgcaggtcgc gaaagcggca gcagcggtgt
     6361 tggcgaaata agcgaaaacg aggagataaa caatgagtca aaccataacc cagagccgtt
     6421 tacgcattga cgccaatttt aaacgttttg tggatgaaga agttttaccg ggaacagggc
     6481 tggacgctgc ggcgttctgg cgcaattttg atgagatcgt tcatgatctg gcaccagaaa
     6541 atcgtcagtt gctggcagaa cgcgatcgca ttcaggcagc gcttgatgag tggcatcgca
     6601 gcaatccggg gccggtaaaa gataaagcgg cctataaatc tttcctgcgt gaactgggct
     6661 acctggtgcc gcaaccggag cgcgtgacgg tggaaaccac gggcattgac agcgaaatca
     6721 ccagccaggc ggggccgcag ctggtggttc cggcaatgaa cgcccgctac gcgctgaacg
     6781 cggcgaacgc tcgctggggc tcactgtacg atgcgttata cggcagcgac atcatcccgc
     6841 aggaaggggc gatggtcagc ggctacgatc cgcaacgcgg tgagcaggtt atcgcctggg
     6901 ttcggcgttt cctcgatgaa tctctaccgc tggaaaacgg cagctatcag gatgtggtgg
     6961 cgtttaaggt ggttgataaa caattacgca tccagttgaa aaatggtaaa gaaaccacgt
     7021 tacgtactcc agcacagttt gtcggttacc gtggcgatgc cgctgcgccg acctgcattt
     7081 tgctgaaaaa taacggcctg catattgagc tgcaaatcga tgccaatggg cggattggca
     7141 aagacgatcc ggcgcacatc aacgatgtta tcgtcgaagc tgctatcagt accatcctcg
     7201 actgcgaaga ttcggtcgcg gcggttgatg cggaagataa aatcctgctg taccgcaacc
     7261 tgctgggcct gatgcagggg actctgcaag agaaaatgga gaaaaacggt cggcaaatcg
     7321 tgcgtaaact gaatgacgat cgtcattaca ccgccgccga tggctctgaa atttctctgc
     7381 acggacgctc gctgctgttt atccgcaacg tgggtcattt gatgaccatt cctgtgattt
     7441 gggacagcga aggcaatgaa atcccggaag gcattcttga tggcgtcatg actggcgcga
     7501 ttgccctcta tgatttaaaa gtgcagaaaa actcgcgcac tggcagcgtc tatattgtga
     7561 aaccgaaaat gcacggtccg caggaagtgg cgttcgccaa caaactgttt acccgcattg
     7621 agacaatgct cggtatggca ccgaataccc tgaaaatggg cattatggat gaagaacgtc
     7681 ggacctcgct gaacttgcgt agctgtatcg ctcaggcgcg caaccgcgtg gcgttcatca
     7741 ataccggttt cctcgaccgt accggcgatg aaatgcattc ggtgatggaa gctggcccga
     7801 tgctgcgtaa aaatcagatg aaatcgacgc cttggatcaa agcctacgag cgtaataacg
     7861 tgctttccgg tctgttctgt gggctgcgcg gtaaagcgca aattggtaaa ggcatgtggg
     7921 caatgccgga cctgatggca gacatgtaca gccagaaggg cgaccaactg cgtgccgggg
     7981 caaacacagc ctgggttccg tcaccaaccg ctgctacgct ccatgcgctg cactaccacc
     8041 aaaccaacgt acagagcgta caagccaaca ttgcccagac cgagttcaat gctgaatttg
     8101 aaccgctgct ggacgatctg ctgactattc cggttgctga aaacgctaac tggtcggcgc
     8161 aagagatcca acaagagctg gataacaacg tgcaggggat tctggggtac gtggtgcgct
     8221 gggtggagca ggggattggt tgttcaaaag tgccggatat tcacaatgtg gcgttgatgg
     8281 aagaccgcgc aacgctgcgt atctccagcc agcatatcgc caactggtta cgtcacggta
     8341 ttctgaccaa agaacaggtg caggcgtcgc tggagaatat ggcgaaagtg gttgatcagc
     8401 aaaacgctgg cgatccggct tatcgtccga tggcggggaa tttcgctaac tcgtgtgctt
     8461 ttaaagctgc cagcgattta atcttcctcg gcgtgaaaca gccaaacggc tataccgaac
     8521 cgttattaca cgcctggcgt ttacgcgaaa aagaaagtca ttaatagcgt caaaaaagcc
     8581 ccggcaagtt ttattgtcgg ggctatgata gtaactgcgg tagtacattt atcccggaaa
     8641 taaccggagc gattatcagg gtttaacaat attaaatacc cgtagcgcat tactagcggg
     8701 cctaaaacaa caatatggac atcttaacga tactctgcgt ctgcgtatgc ggaacagaaa
     8761 cccttacccg atatacttgt tatgccagag gtagcaattg cgttagcggc gtactgttgc
     8821 tgtaaagtat aacgcctata ttttacagtt cagtcctctt ttgcttatta caaccttaag
     8881 tatatcaagc atataaagat aataagagac tgaacaatat ggttacctgg acccaaatgt
     8941 atatgccgat gggaggactg gggctatccg ctctggtcgc cctgatcccg ataatattct
     9001 tcttcgttgc actcgcggta ttacgtctga aaggacatgt cgctggagca ataaccctta
     9061 tattatctat cctgattgca atattcgcct ttaaaatgcc gattgatatg gcatttgctg
     9121 ctgcgggcta tggctttatt tatggattat ggccaatagc gtggattatt gtcgcggcgg
     9181 tgttcctgta taaattaacc gttgccagcg ggcagttcga tattatccgc agctcggtta
     9241 tctccatcac cgacgatcag cgtttgcagg tgttactgat tggtttctcc tttggtgcgt
     9301 tgctggaagg agcggctggc tttggtgcgc cggtggcgat taccggtgcg ctgctggtgg
     9361 gcctgggctt caaaccgtta tacgcggcgg ggctgtgtct gattgccaat actgcgccgg
     9421 tggcgtttgg tgcgttgggc gtgccgattc tggtcgccgg tcaggtaacg ggaatcgatc
     9481 cgttccacat tggcgcaatg gcgggacgtc agttaccgtt cctgtcggtt cttgtgccgt
     9541 tctggctggt agcaatgatg gacggctgga aaggggtgaa agagacgtgg ccagcggcgc
     9601 tggttgctgg gggaagcttc gctgtcactc agttctttac ctctaactat attggtccgg
     9661 aactgccgga tattacttcg gcgctggtga gtatcgtctc actcgcttta ttccttaaag
     9721 tctggcggcc gaaaaatacc gaaacggcaa tcagcatggg acaatccgca ggtgcgatgg
     9781 tggtaaataa gccatcttct ggcggtcccg tgccttcaga atatagtctg gggcaaatca
     9841 ttcgagcgtg gtcaccgttt ttaatcttaa cggtgctggt caccatctgg accatgaagc
     9901 cgtttaaagc gttatttgct ccgggcggcg cgttttattc actggtgatt aatttccaga
     9961 tccctcattt gcatcaacaa gtgttgaaag cggcacccat tgtcgcccaa ccaacgccaa
    10021 tggatgcggt gtttaaattc gaccccctct cggctggcgg caccgctatt tttattgcgg
    10081 cgattatctc tatcttcatc ctcggtgtgg ggatcaagaa aggtattggc gtctttgccg
    10141 aaacgctaat tagcttgaag tggccgatac tgtcgattgg catggtgctg gcgttcgcct
    10201 tcgtcaccaa ctattctggc atgtccacca cgctggcgct ggtactggca ggtacaggcg
    10261 tgatgttccc gttcttctca ccgtttctcg gctggctggg cgtattcctt accggctcgg
    10321 acacctcctc taacgccctg tttggttcac tgcaatcgac cacggcgcag caaatcaacg
    10381 tctctgacac cctgctggtg gcagcaaaca ccagcggcgg cgtaactggc aagatgatct
    10441 ccccgcaatc tatcgccgtg gcctgcgccg cgacgggcat ggtgggccga gaatctgaac
    10501 tgttccgcta caccgtgaag cacagtctga tttttgccag cgttatcggc attatcaccc
    10561 tgctgcaggc gtatgtgttt accgggatgt tagtctcgta aatcaataaa gccggatgat
    10621 cgttaatatc atccggcttc atttacatta ttctgtctta ttaattgaaa atcatttctc
    10681 gctttattat ccatgcagca ataatgtttt gcgttcagat aacctgctaa ataattaaga
    10741 gcgtgagaac atctattttt ttatcacgca aaacaagtct g
//
LOCUS       DAPHBJ010000151        10768 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.153, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000151 DAPHBJ010000000
VERSION     DAPHBJ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.153"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(88..1134)
                     /gene="aroH"
                     /locus_tag="RHZ76_003412"
     CDS             complement(88..1134)
                     /gene="aroH"
                     /locus_tag="RHZ76_003412"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HDV2814000.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(1291..2124)
                     /gene="ppsR"
                     /locus_tag="RHZ76_003413"
     CDS             complement(1291..2124)
                     /gene="ppsR"
                     /locus_tag="RHZ76_003413"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HDV2814001.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            2457..4835
                     /gene="ppsA"
                     /locus_tag="RHZ76_003414"
     CDS             2457..4835
                     /gene="ppsA"
                     /locus_tag="RHZ76_003414"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HDV2814002.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLEANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(4892..6538)
                     /gene="fadK"
                     /locus_tag="RHZ76_003415"
     CDS             complement(4892..6538)
                     /gene="fadK"
                     /locus_tag="RHZ76_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HDV2814003.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(6595..6888)
                     /locus_tag="RHZ76_003416"
     CDS             complement(6595..6888)
                     /locus_tag="RHZ76_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDV2814004.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWKYPAGTFGIDFRYG"
     gene            complement(6885..8174)
                     /locus_tag="RHZ76_003417"
     CDS             complement(6885..8174)
                     /locus_tag="RHZ76_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDV2814005.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEHAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILETNVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(8230..9168)
                     /locus_tag="RHZ76_003418"
     CDS             complement(8230..9168)
                     /locus_tag="RHZ76_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HDV2814006.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLAQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSTLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(9188..9952)
                     /locus_tag="RHZ76_003419"
     CDS             complement(9188..9952)
                     /locus_tag="RHZ76_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HDV2814007.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQAGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            10268..>10768
                     /locus_tag="RHZ76_003420"
     CDS             10268..>10768
                     /locus_tag="RHZ76_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDV2814008.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKN"
BASE COUNT         2592 a         2750 c         2788 g         2638 t
ORIGIN      
        1 attatggctg accagagcaa gcagtaatgt gttgagaaga caacaagttg cgcagaaatg
       61 cccatcccat caggaatggg cacgcattca gaagcgggta tctaccgcag aggcgagttt
      121 ttcgaccagg cgttcggtat cttcccagcc cagacacggg tcggtaatgg attgaccgta
      181 agtgagcggc tgaccgccga cgattttttg cgttccttcg cgcaggaaac tttccgccat
      241 aattccagca atcgccgtag agccattgcg gatttgctga caaatatcct cacaaacttc
      301 taactggcga cggtgctgct tctggcagtt accgtggctg aaatccacca ccagatgttc
      361 aggtaaatca aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg catgataatt
      421 cggttttttg ccgccacgca taataatgtg gccatacggg ttgccgctgg tctgatagat
      481 ggtcatctga ccatttttgt ctggcgagag gaacatatgg ctggcgcggg ctgcgcggat
      541 agcatccacg gcaatccgcg tgttgccatc ggtaccattt ttaaaaccta ccggacagga
      601 gagtgccgaa gccatttcac ggtggatctg actttcggta gtacgtgcgc caatcgcgcc
      661 ccaactgatt aaatcagcaa taaactgacc ggtcaccata tcgaggaact cagtcgcggt
      721 tgggacgccc agctcattta cctgtaaaag taatttgcgc gccagctcca gaccgtgatt
      781 tacccgatag ctgccgttta aatctggatc ggagattagt cctttccagc cgacaacagt
      841 tcgtggtttt tcaaaatagg tgcgcattac gatttccagc cgtgactggt actggttgcg
      901 cagcgactgc agacgggtgg cgtactccat tgcagcggtg agatcgtgga tcgagcaggg
      961 gccaataatg accaacagtc gcttatcttc accattgagt attttttcaa ttctgcggcg
     1021 ggagtcggtg acatgggtgg cgacgccagg cgttacggga taccgtagcg cgagttcggc
     1081 gggcgttacc aggctctcaa tacgcgcagt acggagttcg tcagttctgt tcattacaag
     1141 tctcagaaat ttgttgcttc tacggcagga ctgccgggaa gtgatgcgca tcacgataaa
     1201 ccaatcgctg ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg ttagcatgat
     1261 aacaaaaaaa taagctaatg cactagttct ctagtacatt cggcgactaa ggcccatgat
     1321 atcgaggatc ttggtggcaa tctcttctac cgaataattg gtactgttaa tccacgggat
     1381 ctgattttta cggtacaagg cttccacttc cgcgacttcc atcctgcact gacgaagcga
     1441 ggcatagcga ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt ccgggtcgat
     1501 agtcaggccg aacaatttat gctgaagcgg tttgagcgac gcgggtagca ccagattatc
     1561 catatcgtcg gcaataaagg ggtagtttgc cgcgcggata ccaaattgca ttgccagata
     1621 cagactggtg ggggttttac cacagcgaga aacaccgagc aggatcacct gagcctgatc
     1681 cagattgcgc aacgaaatgc cgtcatcgtg ggcgagggtg taatcaatcg ccgcaatgcg
     1741 cgcatcatat ttattgagat tattagggtt aaggccatgg gtacgatgag caatcggcgt
     1801 tggatccagt ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga tatcctggca
     1861 aaacccttca ctttgcaaga tgatggcgcg aatctccggc aacacgatgg agtagaagac
     1921 cagcgggcgc acacctgtct ggtgataaat tgcgtcaatc tgatccttca ctgcccgtgc
     1981 acggctctca ttttcgacaa acggcagcgt gatgctgctg atagtgacgg gaaattgtga
     2041 cattactgcg tgtcctaata cctccgcagt tattgccgta ccatcagaaa tataaaaaac
     2101 gtggcgatca acagcattat ccattttgtt cttcccgtga tgcagacata attctatgaa
     2161 agcataaatt aaaaacgcac agaagcgtag aacgttatgt ctggtttata aaatgaacct
     2221 tcaattttat tttttatgaa aacagtattt catttttatg gtttcgttta taccgatggt
     2281 ttatgtggaa attgtcgaag agagcagatt tgcgcaacgc tgggatcagt cttaaaaagt
     2341 aaaaaaatat atttgcttga acgattcacc gtttttttca tccggttaaa tatgcaaaga
     2401 taaatgcgca gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt
     2461 ccaacaatgg ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag
     2521 acagggttgg gggcaaaaat gcctccctgg gtgaaatgat tactaacctt tccggaatgg
     2581 gtgtttccgt tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc
     2641 aaagcggcgt aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta
     2701 cccagcttgc gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc
     2761 ctgagctgga aaacgccatc cgcgaagcct atgctcagct ttccgccgat gacgaaaacg
     2821 cctcttttgc ggtgcgctcc tccgccaccg cagaagatat gccggacgct tcctttgccg
     2881 gtcagcagga aaccttcctc aacgttcagg gttttgacgc cgttctcgtg gcagtgaagc
     2941 atgtatttgc ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg
     3001 atcaccgtgg cgtggcgctc tctgccggtg ttcagcggat ggtgcgctcc gacctcgcat
     3061 cttctggcgt gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca
     3121 cttccgcatg gggccttggg gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt
     3181 acgtccataa accgacactg gaggcgaatc gcccggctat cgtgcgtcgc accatggggt
     3241 cgaaaaaaat ccgcatggtt tacgcgccga cccaggagca cggcaagcag gttaaaatcg
     3301 aagacgtacc gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac
     3361 tggcaaaaca ggccgtacaa attgagaaac actacggtcg cccgatggat attgagtggg
     3421 cgaaagatgg ccacactggc aaactgttca ttgtgcaggc gcgtcctgaa accgtgcgct
     3481 cacgcggtca ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag
     3541 gccgtgctat cggtcatcgc atcggcgcgg gaccggtgaa agtcatccat gacatcagcg
     3601 aaatgaaccg catcgaacct ggcgacgtgc tggttactga catgaccgac ccggactggg
     3661 aaccgatcat gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg
     3721 cggcgatcat cgctcgtgaa ctgggcattc cggcggtagt gggctgtggt gatgcaacag
     3781 aacggatgaa agacggtgag aacgtcactg tttcttgtgc cgaaggtgat accggttacg
     3841 tctatgcgga gttgctggaa tttagcgtga aaagctccag cgtagaaacg atgccggatc
     3901 tgccgttgaa agtgatgatg aacgtcggta atccggaccg agctttcgac ttcgcctgcc
     3961 tgccgaacga aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt atgattggcg
     4021 tccacccacg cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc
     4081 gcgagatgat gaaaggtttt gattctccgc gtgaatttta cgttggtcgt ctgactgaag
     4141 ggatcgcgac gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt
     4201 ttaaatcgaa cgaatatgcc aacctggtcg gtggtgagcg ttacgagcca gatgaagaga
     4261 acccgatgct cggcttccgt ggcgcgggcc gctatgtttc cgacagcttc cgcgactgtt
     4321 tcgcgctgga gtgtgaagca gtgaaacgtg tgcgcaacga catggggctg accaacgttg
     4381 agatcatgat cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg
     4441 cgcgtcaggg gctgaaacgt ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc
     4501 cgtccaacgc cttgctggca gagcagttcc tcgaatattt cgacggcttc tcaattggct
     4561 caaacgatat gacgcagctg gcgctcggtc tggaccgcga ctccggcgtg gtgtctgaac
     4621 tgttcgatga gcgcaacgat gcggtgaaag cactgctgtc gatggcgatt cgtgccgcga
     4681 agaaacaggg caaatatgtc gggatttgcg gtcagggtcc gtccgaccac gaagactttg
     4741 ccgcatggtt gatggaagag gggatcgata gcctgtctct gaacccggac accgtggtgc
     4801 aaacctggtt aagcctggct gaactgaaga aataaaataa atccccggcg gcgttcagtc
     4861 gccggggtta tgtgatcccc gaagatgata cttattcaat ctcttcacag acatcctgcg
     4921 ttaatcgccg cataatatct tttcttaaca aaaacttttg tattttacct gaggcagtgc
     4981 gcggtagttt ttcgattacc acgatatgtt caggatattt atattttgcg acccgtttac
     5041 ggctaaaaaa agccaccacc tcttccagcg ataatgaatg atgcggcgct ttcagcacga
     5101 cataagcgca tgatcgttca cctaaacgtt catcgggcat tgcaaccaca caggcatcgt
     5161 gaattttagg atgctgcaat aaaatatctt ccacttcacg gctgctaata ttttcgccgc
     5221 cgcggacaat aatatctttt ttgcgtccgg taatttttat atagccagcc tcatccatgc
     5281 ggcagagatc gccgctgtaa taccagcctt cttcatccag cgcacgggcg gttaattcag
     5341 gttcatcaaa ataccccata aacacattgg ggccacgcga ggcttcttca ccttcacaac
     5401 ctggcggtaa ggtcttgcgt gcgtcatcga ccactttaat ctctacacct gcggcagcgt
     5461 aaccatcggt gtgcataaag cgcgacaaag gatcatcgag attcaccacc gcatgcggcg
     5521 aactttctgt ggaaccataa acacttaata atttaatgcc gcgctgctgg cattcacgcg
     5581 ccactttttt ggggattgtg gttccgccgc aaagaaagaa acgcagcgct gaaaggtccg
     5641 cgggttgttt ctctagtaca ttcaaaagat catagacaaa cggcgttgcg ccgagcatac
     5701 aggtgcaacg ctgctgctca agcagcgcga gacacgcatc aggagtgaaa atatctaaca
     5761 acacgctgcg agcaccaatc aaaaatggtg ctgttacgcc atgcagaaag cccgttgcgt
     5821 ggccgagtgg cgcaggcatc ataaagacat cctgccaggt cagattcagc cgcgcgcaat
     5881 aagcccgctc actggcgaga atattgttat gcgttagcat cacgcccttt ggcagaccct
     5941 cggttccgga ggtaaacagc accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg
     6001 tcagtggggt attgtcggcg ataatctgac ttaatgagag ggaagaggtg gcgggagcca
     6061 gtttgtccac gccgacaatt tgttgtagtt gtggaagctg attttgcagc ggcaggatta
     6121 aatctaccgg gcgcgtttgt ttaaacaacg tcggtgcaaa gaacattttt gcctgacact
     6181 tattgagcac ccacaccagt tctgcttccc gccaggaagg caacagcggc acggaaaccg
     6241 caccgatttt caagcaggca agatagataa cggtaaattc acaccagcca ggcagttgaa
     6301 atgcgatgcg atcgcctgat tcaataccct tcgctaacat ccagtttgcc agacagctcg
     6361 cggcgtgatc gagtgcgcta taggtgtacg atgctccatg attatcgacc acggcaattt
     6421 tgtctggcat cgcacgagcg gtctgctgcc agtaatcggc cagcgaagca tcgccccata
     6481 gcccttgctg acgatacgcc gcacgacgtt gttcgttaaa cgttaatgtc actttcattt
     6541 tggactctcg aaacagaaca tcaggcccga tgtgcgggcc tgtgggatgc gtgattagcc
     6601 gtaacggaaa tcaataccga aggtacctgc gggatatttc cactgttcga gaatagtgtt
     6661 accgcacaac acccgacagg tgccgcactc cagacaaccg gcagaatcaa aatgaatgtt
     6721 gccggcgtca tcctgcttat aaagtccggc agggcaggct ttcattaatt tacggaattc
     6781 attgatatcg ggatttgccg ccaaaatgat atgcgggtgg ccctcatcaa catggaattt
     6841 attgacgcct aatttgatgt cgacgttaac cgtagcgttc tggctcatag cgcggttgct
     6901 cccttaatgc catctttcag caagttgatc agcccgattt tcttcgcatg tcccatgatc
     6961 attttgcgta ccggctggtt tggtttgccg tcaatggtga acatctcgtt catgatgtcg
     7021 gctaccattc gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg
     7081 cgaaaatgct gcatatcacg catgacgcag ctttgttcca gctcacgttt gtattgcgca
     7141 agactgctgg cggagaaatc cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc
     7201 tgagccgatg caatggctaa atccatgccg cggaccgtaa aacccaaatt caggcagaag
     7261 cctgcggcgt caccaacgat catcacgccc tcgttaacca gttgcggcac cattgccaga
     7321 ccgccttccg gcaccatatg cgcggaatat tcaagcagtt tgccgccgct aatcagcggg
     7381 cgaatggcgg ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg
     7441 gcgatatcac ccaatccaca aaccagcccc aacgatatgg aatccttatt ggtatagagg
     7501 aatccgccac ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc
     7561 tcgcccgtaa tattaaagcg atcgttgatc tgttctggtg tgaggccaat aacctcttta
     7621 acaccaacag cgtaatgatg cggatcggaa gcgggaacca ttcccagcga gcggccaagc
     7681 atcgagttaa cgccatcagc cagaatcacc acattcgttt cgagaatatc atccccagcc
     7741 tggacgccag tgaccttgtt tccttcacga accagcgcat cgacgcgaac tcccgggata
     7801 aactgtgcgc ccgcctgctc ggcatgttcc atcaaccacg ggtccagacg attacgcaat
     7861 acggtataag atgcgtgttg cggaacatct ggttgctcgc ggtgaaaatc gagggtaacg
     7921 gcgctctctt cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc taccggcgct
     7981 gatgctgcaa agcctggaat gattgcttca agtgtgtggg cataaaggcg cccgccggtc
     8041 atgtttttac atccggcact gtcgccgcgt tctatcacca ggacatccag cccggctcgt
     8101 gccatgacca gtgcggcaac gctaccagca acgcccgcac cgaccacaat ggcatcaaat
     8161 ttgtcatccg acatgcgtta tgctcccttc ccgctgcgtt tacagcggga gatgtggttg
     8221 aaaggggaat tagcggctca actggctaat cagggcaggg acgactttgt agatatcgcc
     8281 caccagaccg tagtcggcat agttgaagat tggcgcattt ttatctttat tgatggcgac
     8341 aatcactttt gcgccgttgc cgccaaccat atgttggatc tgcccggaga tccccagcgt
     8401 caggtagaga tcggatttca gcaacacgcc ggagacaccg atataacgtt cacgctccat
     8461 ccagttctcg ccttcggcaa ttggacgtga acagccgact tcagcattca gcaccgccgc
     8521 cagttcgtgg accattttca ggtcatcctg cgccgccaga ccacgaccga cgccaaccac
     8581 acgttttgct ttgctcaggt ccacgctgct tgcggctttc gcacggcgtt cgcgacagag
     8641 aatttcatga cgcggagcaa cataaggtac cgtttctgtc gggcactgat gagaggtatc
     8701 actggtgcac ggttcctgaa caccgggtgc aagggtaatg atcgctagcg tgctgttgat
     8761 cttttcctga gcgaacgcta acccgccata catccggtgt tcggcgcaaa tgtgaccatc
     8821 gacaatatcc accgccgtgg catcgttcac cagcgccgca ttcagttgca cacttaaccg
     8881 tgctgccagc gctttaccac gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc
     8941 tttcagcagg gcagcaatgc tttcggcgta attttcagtg cgttgcagcg cgtcgttttg
     9001 cgcaagaaca taaatacatt ttggaccata aggcataact gcctgcgcct ggtcggtatt
     9061 ttgtacaatg gcatacacct gttggcccca ttgctgagcg ccgccaaaca gttcagcata
     9121 acgttcagga ttatcgctaa agacccagac gctgtttaat tgactcatcg tttcttctcc
     9181 gtaggcttca gttcagggct ttcttcagat gttccgccag ctcggcaatg gcctccggcg
     9241 aatcgttatc gagaatgatg tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca
     9301 gttcagcaag tggcgcgctc tggctccagc caatatcact tgcctgccac tgatttaccg
     9361 gttttttacc cgcgccaaga atggctttca tcgaaggaat gcgtggcacg ttaatatcgg
     9421 aggtgacgca gagcacggct ggaacagaga gttcaataac ttcaacatca tcttcaagcg
     9481 tacgttcaat caccagtgta ttgccctgac gctgaatagc actcactgca ttaatcaccg
     9541 gaagttgcag aatttctccg accagcaagc cagcctgctg ggcataaagg tcgccggaac
     9601 cttcaccaaa gatcagtaaa tcgaagccga tcttttcaac tgctgccgcc agcgcttttg
     9661 cggtatcgag aggcagtgca tgctcaagtt gtgcatcctg caccatatac aggctgtgcg
     9721 gcccgcggga tagcacgtct ttgcgcactt tcgagttctg caacaatgag ccgccaacgg
     9781 tcagcgcggc tatctcatcg tcatctgttg caagctggct tgcagcttca atggcattga
     9841 gatcgaactg gctgattttg gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga
     9901 caacaatgtc ctgttcttca ggcaccagct taaagcaggt tattattttc atgatttctc
     9961 ctgtgagtcg catacggaac catctccgat ggaaggtaaa aatggcctga aaatagtgtg
    10021 cgatagcggt gcggggtttt cgacggagca agctgttttc cttgccaaca atcttgcaaa
    10081 aaaaatgcga tcgctaaaac aaaagtgcaa tttgcggcaa tatagttatt ccatgcccgt
    10141 acaatcttgc attcagtgta aataatcttg tttagctctc gctaaacgcg gttgtataac
    10201 cgcaatacac gccgtatttt atccggcata tattgttttt gcttgctcat ggaataggga
    10261 ggttgtcatg tatcaacgct gttttgataa tgccagtgaa acgctgtttg tcgccggtaa
    10321 aacgccacgg ctttcacgtt ttgcatttag cgatgatcca aaatgggagt ctggacatca
    10381 cgttcatgac aatgaaaccg agctgattta cgtcaagaaa ggggtagcaa ggtttaccat
    10441 cgattcttcg ttatatgtcg cgcatgccga tgatattgtg gtgatagaac gcggcaggct
    10501 gcatgcggtg gcctctgacg ttaacgatcc ggcaacgacg tgtacctgtg cgctgtacgg
    10561 ctttcagttt cagggggctg aggaaaatca gctactgcaa ccgcattctt gcccggtaat
    10621 tgccgcaggg cagggaaaag aagtcattaa aaccttattt aatgagctaa gtgtgatttt
    10681 gccgcaaagt aaaaatagcc aaacatcttc gttatgggac gcatttgcct atacgttagc
    10741 aattctttac tacgaaaact ttaaaaat
//
LOCUS       DAPHBJ010000152        10747 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.154, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000152 DAPHBJ010000000
VERSION     DAPHBJ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.154"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            134..847
                     /locus_tag="RHZ76_003421"
     CDS             134..847
                     /locus_tag="RHZ76_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDV2814009.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     HPADEILQASLRAIHAALS"
     gene            879..1637
                     /locus_tag="RHZ76_003422"
     CDS             879..1637
                     /locus_tag="RHZ76_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814010.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1683..3032
                     /locus_tag="RHZ76_003423"
     CDS             1683..3032
                     /locus_tag="RHZ76_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HDV2814011.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEVYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            3032..3856
                     /locus_tag="RHZ76_003424"
     CDS             3032..3856
                     /locus_tag="RHZ76_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814012.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPVALDSDIRQRLELLISRFPL"
     gene            3868..4428
                     /locus_tag="RHZ76_003425"
     CDS             3868..4428
                     /locus_tag="RHZ76_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDV2814013.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            4459..5529
                     /gene="lptF"
                     /locus_tag="RHZ76_003426"
     CDS             4459..5529
                     /gene="lptF"
                     /locus_tag="RHZ76_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDV2814014.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLIDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            5526..6605
                     /gene="lptG"
                     /locus_tag="RHZ76_003427"
     CDS             5526..6605
                     /gene="lptG"
                     /locus_tag="RHZ76_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDV2814015.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSMGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMDLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPIAFVLVALSLVYWYDRQH"
     gene            complement(6640..7812)
                     /locus_tag="RHZ76_003428"
     CDS             complement(6640..7812)
                     /locus_tag="RHZ76_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDV2814016.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSYISPVVPVIIGSKE
                     DGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(7812..8060)
                     /locus_tag="RHZ76_003429"
     CDS             complement(7812..8060)
                     /locus_tag="RHZ76_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDV2814017.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(8092..9006)
                     /locus_tag="RHZ76_003430"
     CDS             complement(8092..9006)
                     /locus_tag="RHZ76_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDV2814018.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETIQTQTYELCDGVAGGYDW
                     QRVQQLAADARCGSVRMVGIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(9003..10733)
                     /locus_tag="RHZ76_003431"
     CDS             complement(9003..10733)
                     /locus_tag="RHZ76_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HDV2814019.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRC
                     QLEDQLEYQTLKARAEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLFNAATHDNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPG
                     DRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQASQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
BASE COUNT         2583 a         2687 c         2811 g         2666 t
ORIGIN      
        1 tttggttaat atgtttttat gaaagcgtaa ttcaggtcaa tgtcacaatt aaccattgtc
       61 acaataaggt tgaacggata ttctgagcac ggaccacgta atattcaata cattatttac
      121 tgccgggttt ttcgtgaatc aacgtaatat gtcaataatt aattccacgc cagtgcgtgt
      181 tattgccatt gtaggatgtg atggttcagg taaatcgacc ctcacggcaa gcctggtaaa
      241 tgaactggca gcaagaatgc caacagaaca catttatctc gggcaatcgt ccgggcgaat
      301 tggcgaatgg atttcacagc tccctgttat tggcgcacct tttgggcgtt atctgcgaag
      361 taaagcggca catgtgcacg aaaagccctc aacaccgcct ggcaatatta ctgcactggt
      421 tatctatctg ctttcctgct ggcgggcgta caagtttcgc aaaatgttgt gtaaaagcca
      481 gcaaggcttt ctgctcatca ccgaccgcta cccgcaggtt gaagtgccgg ggtttcgctt
      541 tgatggcccg caattggcaa aaaccacggg cggtaacggt tggataaaaa tgttaaggca
      601 gcgcgagctg aagctgtacc aatggatggc atcttatttg cccgtattgt tgattcgtct
      661 tggcattgat gaacaaaccg cgtttgcgcg taaacctgac caccaactgg cagcgttaca
      721 ggaaaaaatc gccgttacgc cgcaactgac attcaatggc gcaaagatcc ttgaactgga
      781 tgggcgacac cccgccgatg aaattctgca agcgtcacta cgcgcaattc acgccgcgct
      841 ttcctgatca ccaaaatgga taggggatga tttaacgaat ggatgcacta caaactcaaa
      901 ctgttaatag tacaaccgcg ccgcagccca actacattcc ggggctgatt gcggtggtcg
      961 ggtgtgatgg caccggtaaa tccacactga ccaccgacct ggtgaaatcg ctgcaacaac
     1021 actggcaaac cgagcggcgc tatctggggc tgctctccgg cgaagacggc gacaaaatca
     1081 aacgattgcc gttggttggc gtctggctgg aacggcgact ggcggccaaa tcctcgaaaa
     1141 cccaaagcat gaaaaccaaa tctccggcgc tatgggcggc ggtgattatg tactgcttct
     1201 cgctgcgaag aatggcgaat ctacgcaagg ttcagcgact ggcgcaaagt ggcgttctgg
     1261 tggtcagcga tcgcttcccg caggctgaaa tttcgggctt ttattatgat ggaccgggga
     1321 ttggcgtcga acgtgcgacc gggaaaatca gcatgtttct ggcgcagcgc gaacggcgtt
     1381 tataccaaca aatggcgcaa tatcgcccgg aattaattat tcgcctgggc attgatattg
     1441 agactgccat ctcccgcaag cctgaccatg actatgccga gctgcaggac aaaatcggtg
     1501 tcatgtcgaa gattggctat aacggcacaa aaattcttga gattgattcc agagcgccct
     1561 acagcgaagt gctggaacag gcacagaagg cggtttctct ggttgccatc gtttctgacc
     1621 gccgaagttt aacttaaggc tggcgggata ctttttacta tcaacgataa ggtcaggctg
     1681 aattggcggg ttttaacatc aaacattggt ttgcagatgg cgcgtttcgc accattattc
     1741 gcaatagcgc ctggttaggc tccagtaatg tcgtgagcgc cttgctgggt ctgttggcgc
     1801 tctcgtgtgc cggtaaaggg atgacgcccg ccatgtttgg cgtactggtg attgtgcaat
     1861 cgtacgccaa gtcgatcagc gattttatta agtttcagac atggcaactg gtggttcagt
     1921 acggaacacc agcattaacc aacaataatc cgcagcaatt ccgcaatgtc gtctcatttt
     1981 ccttctcgct ggatatcgtc agcggcgcgg tggcgattgt cggtggcatt gccttactgc
     2041 cattcctttc ccattcatta ggtctggatg accaaagttt ttggctggca gcgctctatt
     2101 gcacgctcat tccttcaatg gcttcctcca cgccgaccgg cattctgcgt gcggtagatc
     2161 gcttcgattt aattgctgta cagcaggcga cgaaaccttt tctgcgcgca gcggggagcg
     2221 tcgtagcctg gtattttgac tttggttttg cgggttttgt tattgcctgg tacgtgtcga
     2281 atctggttgg cggcaccatg tactggtggt ttgccgcgcg cgaattacgc cgccgaaata
     2341 tccataacgc cttcaaattg aatctgtttg agtctgcccg acacattaaa ggcgcgtgga
     2401 gttttgtctg gtcaaccaac attgcccact ccatctggtc ggcgcgtaac tcgtgcagca
     2461 cagtgttggt ggggatcgtg ttaggacccg ctgccgccgg gttatttaaa atcgccatga
     2521 cattcttcga cgccgccgga acgccagcgg gtttgctggg taaaagtttt tacccagagg
     2581 tgatgcgttt agatccgcgc accaccagac cgtggttgtt aggtgtgaag tccggtttac
     2641 tggcgggcgg aatcggtatt ctggtggcgc tcgcggtgtt gatcgtcggc aagccgctca
     2701 tttcgctggt gtttggcgtt aagtatctcg aagtgtatga cctgattcag gtgatgctgg
     2761 gcgcaattgt gatttcgatg ctgggcttcc ctcaggagtc attgctattg atggcgggaa
     2821 aacagcgcgc ttttctcgtg gcgcaaacca tcgcctcaat cggctacatc gtattgctgt
     2881 tcatgttctg tcatctgttt ggtgtgctgg gagcagcgtt tgcttacttc ggtgggcaat
     2941 gtctggacgt ggcgctctcg ttaattccaa ccttaaaggc attttttcag cgccattcct
     3001 tgctttataa cgcagccgga gagaaatcct gatgatgaga aaatatttcc cgcttgaggc
     3061 gagcgagcgt ctatttgttg cgattgaaga agatgatgtc gttgatgcac aggttagttt
     3121 gccgccgact attgcactaa gttgtaccac cgaaatcatt catgataatt acgcgttatg
     3181 cctgcaattc tggctcaatg gcgttgatcg tcaggagtta ctgcgtttag ttcgcaaaca
     3241 agccaaaggc gatgagctga ctgctgatga gcgcaaacaa tttaaataca tgcgagcgcg
     3301 ctacaagcac ctgcgttttg cgcaacggct gtatttaaag aagcatcagg ccggattttt
     3361 attcggcaaa acgaccgttt ttctggggcg ttttcaggac ggctttcgta acggtaagaa
     3421 aaatattgtg tcgtattacg gcaacctgtt acggatttat ttaagctctc cggtctggtc
     3481 gttggtgaac tattcattac gccatagcca gttagaaagc gtaagcagtt ttattgccta
     3541 tcggcaaaag caaatgcata ccctgaaaga gattattgcc aaaccgcgac taaccggtag
     3601 ggaatttcat gatgtgcgaa aaatcatcag ccagcaggtt tcttattacg acaccttaag
     3661 atcgcttgat cctgaaaata aagaggcgtt acagatatcc cgcttcctcg ccgccattaa
     3721 tggcctgatg ggagataaac acgacgacat ggtggcggat gatatggaaa accgccagtc
     3781 gtatgatgca ccggtggcgc tggatagcga tattcgtcag cgcttagagc tgctgatttc
     3841 gcgttttccg ctatagccgg agcaatgatg tcatctcgtt tgattattgc gttgattatc
     3901 atgctcttag ccccgggtgt gcaggcacac aatttcgtca ctggcaaaac ggttacccct
     3961 gtttatatcc aggaaggcgg cgagctgctt ctgaatagcg acgacgaaat tcactaccaa
     4021 aagtggaaca gcacacaatt agcgggaaaa gtccgcatta ttcagtatat cgccgggcgt
     4081 aagtcggcga agaaaaagaa ctcactgtta attaaagcgg ttgaagcggc aaactttccg
     4141 caggatcgtt ttcagcccac caccattgtg aacaccgatg atgccatctt tggtactggc
     4201 tattttgttg ttggcaaaat cgaaaaaaat aaacgccgct atccgtgggc gcaattcgtt
     4261 attgacggca atggacaggg acgcgttgcc tggcgcttgc cggagcagag ttcaaccatt
     4321 ctggtgctga ataaagccgg gcaaattcag tgggcaaagg acggcagcct gacgccggaa
     4381 gaggtagatc atgtcattgc gttggcgcag aaactgatca atgaataaaa aactcaaagg
     4441 attgagggcg ttagtgagat gaagctggtt gaacactaca tcatgcgtgg tacgcgccgt
     4501 ctggtgctga ttatcgtcgg ttttctgatc tttattttcg ccagctactc cgcacagcgt
     4561 tatctgaccg aagcggcaaa tgggacatta gctcttgatg ttgtgctgga tatcgttttc
     4621 tacaaagtac tgattgcact ggagatgttg ttacctgttg gtctgtatgt gtcagttggc
     4681 gtgacgctag ggcagatgta taccgactcg gaaattaccg ctatctctgc ggcggggggc
     4741 agtccgggac gcttgtacaa agccgttctt tatctggcga taccgctaag tatttttgtc
     4801 acccttctgt cgatgtatgg tcgtccgtgg gcttatgcgc agatttatca actggagcag
     4861 cagtcgcagt cggagctgga tgttcgccag ttgcgggcaa agaaatttaa cactaacgat
     4921 aacggacgaa tgatcctttc gcagacggtt gatcaggata ataatcgcct gactgacgcg
     4981 ctgatttata cttctactgc caatcgaacc cgcattttcc gcgcccgttc ggttgatgtg
     5041 gttgacccat cacccgagaa accgaccgtt atgttgcaca acggcactgc ctatcttatc
     5101 gatcatcagg ggcgtgatga caacgaacag atctaccgta atctgcaatt acatctgaat
     5161 ccgctggatc aaagccctaa cgtcaaacgc aaagcgaaat cggtcacgga gctggcgcgc
     5221 tccgtctttc cagccgatca tgccgaactg caatggcggc aaagccgtgg cctgaccgca
     5281 ttgttgatgg cgctgctggc catttcatta agtcgggtaa aaccgcggca agggcgattt
     5341 tcaacgttat tgccactgac gttgctgttt attgccattt tttatggcgg tgacgtctgc
     5401 cgtacgctgg tggctaacgg cgcgattccc ctcattcctg gtttgtggtt agtacccgga
     5461 ctcatgctaa tgggcctgct gatgctggtc gcacgcgact tctctttgct gcagaaattt
     5521 tcccgatgaa tgttttcagc cgctatttaa tccgtcatct ctttctcggt tttgccgccg
     5581 ccgcagggct attgctgccg ctttttacca ccttcaacct gattaacgaa ctggatgatg
     5641 tcagcccggg cggttatcgc tggactcagg cggtgctggt ggtgctaatg accttaccgc
     5701 gcacactggt cgaactttcg ccatttatcg ccttattggg agggattgtc ggcctggggc
     5761 agttatcgaa aaacagtgag cttaccgcca ttcgcagcat ggggttttct atcttccgta
     5821 ttgcactggt ggcgctggtt gcagggatat tgtggactgt ttcgttaggc gcgatagatg
     5881 agtgggtagc gtcgccatta cagcaacagg cgttgcaaat caaatcgacc gccaccgcgt
     5941 tgggggagga cgatgacatt accggcaata tgctgtgggc caggcgtggt aatgaatttg
     6001 tgacggtgaa atcgctgaac gagcagggcc agccggtggg cgtggagatt tttcattatc
     6061 gcgacgatct ctcgctcgaa tcctacattt atgcacgcag tgccaccatt gaagacgaca
     6121 aaacgtggat cctgcatggt gtgaatcata aaaaatggct gaatggcaaa gaaacgctgg
     6181 aaacattaga taatcttgcc tggcaatcgg ccttcaccag tatggatctt gaagagttat
     6241 caatgccggg caatactttt tctgtccgtc agcttaatca ttacatccat tatttgcagg
     6301 aaaccgggca acccagcagc gaataccgcc ttgcactgtg ggaaaaactg gggcaaccca
     6361 tcctgaccct ggcgatgatt ttgctggctg tgccgtttac ctttagcgcc ccgcgctcgc
     6421 cagggatggg tagccgtctc gctgtaggtg tcatcgttgg cttactcacc tggatcagct
     6481 atcaaatcat ggtcaatctg ggattgctat ttgcgttgag cgcacctgtt accgcgctcg
     6541 gtttaccgat agcgtttgtg ctggtggcgt tgagcctggt gtattggtat gacagacaac
     6601 attaaacccg ccgtggcgca acaccacggc gggctcacat tactgcttca gagtagcaaa
     6661 tgcctgaatg atggcatcga tttgctcacg actgtgagca gcgtttacgc tacagcgcag
     6721 cagcgtgatg cctgcaggcg cggcaggtgg caggataaga ttcacataaa cgcccagcga
     6781 aataagctcg cgccagatgc gtaaaccgtc ctctttcgaa ccgattatta ccggcactac
     6841 cgggctaata tatgaactta actcataacc cagtttttgc agaccgtcat acagatggtt
     6901 ggcattatcc atcagctttt gccgcagctc tggttgtgtg gcaatagttc ttaacgacga
     6961 gcgcacggtc gcaatgcagg acggagaagg agaggcggta aaaatatacg ggcggctgcc
     7021 gtagcgcagc acttccatcg cttccgaacc gacagcaaaa ccgccaatcg aggccagact
     7081 tttactgaag gtgccaacga taatatccac atcatcttca acgccgacag cttcagccag
     7141 tccgcgcccc gttgcgccta acacgccaaa cgaatgcgct tcatcgacaa tcaaataacc
     7201 accgagacga cgtttgatat caacaatttc cgctaacggc gcaacgtcac cgagcatgct
     7261 gtagataccc tcaacgatga taatcgcctc tttcgcccgt tcaccgaggc gaaccatgcg
     7321 ccgttccaga tctttagcat cgttatggcg gaaacgaata atttccgcgc caccgagaga
     7381 acaggcgtca taaatactgg cgtggctgtc ggcatccagt aacaccacgg cgttatggtc
     7441 ggcaagtgcg ctgatgacgc ctaaattagc ggtataaccg gttgaaaaaa cgatagcggt
     7501 agggcgattg aaaaactcgg caatctcttt ctctaacgcc aggtgcggcg cataactgcc
     7561 gtttgccata cgagaaccgg tggtgccggt accttgggcc gctaacgcgg cctgaccttc
     7621 gctgatggca tcatgattaa aggttaaacc gagataatta ttggtcccgg ccagaatcac
     7681 ttttttattg ccgatccggc cttcggtggc ggaatacact tcatcaatac aggtgccaaa
     7741 cggtgttaac ccgttgtcac taaattgcag tcgctcgcca gcaaggcgcg catatttatc
     7801 gtatagcccc attatttatt ctccagccat ggaagtagag tttccattaa ttgcgcaggg
     7861 gttttgacat ccagcagaat attaatagga atggaaatat cgaatctgtc ttccagcatc
     7921 attaagagat ccatcacttt gatcgactcc aggccaagat cgttgacgag atcgctgtcg
     7981 ggcttaattt ccacaccgtt ttcgaccaaa tcctgtaaac aggaaaggat gtaatccatt
     8041 actatttcac gatttaccat agaaacgtta acacaccttt aaacaagaca atcaaaatag
     8101 cccgttgcgt aatgcgtgtt ccaggctaat cccgggaaac caattaatat cttcagaaat
     8161 acggttatta cttgccgacc agtcggcgtg ggttaattca cgaattttcg agcgggtcag
     8221 cataggttct ttacctgcca ggcgactcaa cgcggtactg atatccgcaa ggccggtgag
     8281 taccggcaga ggaatgccaa ccattcgcac ggaaccgcaa cgggcgtcgg cggcaagttg
     8341 ctgtacgcgt tgccagtcat agccgccggc gacgccatcg cataattcat aggtttgcgt
     8401 ctgtatagtt tcggcgctta accactgacc cactgcttgc gcgaaatcgg tgacgtgcag
     8461 aaaagagagc tgtgtatctg gcgcaccaag tcgtggcagc aggccgcgca gcatccagtc
     8521 aaacagcggt tttaactctt tatcgccggg accatacacg gctgtcgggc gaaaaacgcc
     8581 cagcgtaatt tcgtccgcca ttgctgccag tcgttgctcg gcgacgtatt tggattttgc
     8641 ataccaggag agctcgggat ggcgcgccgc cagcgaagag ataaacagaa aacgttggca
     8701 aaagccgctc tcttttgctg cttgcatcag gcgcaggctg ccgtcaacgt tacagcgggt
     8761 gaaaatctct tctttgtgcc cgcgcacttg cccggcgcaa tggaccacta cgctggctcc
     8821 ggcaaccagc tcgctaagtg aatgagtatc ttccagcgaa ccgcgcaccc aggtgagatt
     8881 atcattgacg tgagcgcggg cagtacgcgt caatgcgcga acgtgaaagc cgcgggcgag
     8941 caggttatcg ataatatatt taccgataaa cccggtagcg cccgtcaccg cgacagtttg
     9001 attcatgcca gggattcctg cacattaaga ctggcagcat aagccttctg ataacgtttt
     9061 ttcgcttccg cacgggcagg cttgccggag gacgttcggg gaatactgtg gggcggcaac
     9121 agatcgatag ccgcggtcac gccaaattcg ctttggatcc gtgccgccag cgcgtggata
     9181 agctgcccgc gacgttcttc gtcgctgatc cgacactgga tctgcaaaat gattttttcc
     9241 tgggcggtaa caaaagcaat cgcatcgcca gaatgaattt ccggttcctg ttccgctata
     9301 tattcaatat cctgcggcca gatattacgg ccacgaataa taatcagatc tttaatgcgt
     9361 ccggtgacat acagataacc gtccagcaga taaccgaggt cgccggtgtc taaccagccc
     9421 gtcgcggcaa tctcgtcttg cgaagcctgg tcgccaaagt aaccgctcat cagactggga
     9481 ccggagatgc aaatatggcc taccacacgt tccgcgaccg gcatacctgc ttcattgcgg
     9541 atttcaatac catgttccgg caacgctttg ccgcagttga cgaaagtcga tacggcgcgt
     9601 gtctctgcac ccggcgcgac ggctttaccc tgatattcga ggatgtcgcg atccacttcg
     9661 ttaaccacaa ccccggaggc ttcatcagag aagctgacag ccagcgcatt ttctgccagt
     9721 ccgtagcacg gcatgaaagt tttattgtca aagttaacct gacggaaaca ttcagcgaat
     9781 tgatggagtt gttctgcgga gatgggttct gcaccaatac cagcgacgcg ccagcaggaa
     9841 agatccagtt cagcgagatc tttttcattc acgcggcgct ggcacaattc atagccaaac
     9901 ggcggcgcaa cggaaacggt gccgcgattt ttactgatca atttaagcca ttgcagagga
     9961 cgcatggcaa aatcctgagt gcgcaaataa tctactgaaa gctgcgtggc gacgggggtc
    10021 aggagaaagc cgaccagtcc catatcatgg tagaaaggca gccaggagac gcagcggtcg
    10081 ccagggcgta atttaatgcc gtcgtggctt atagcacgta gattagccat cacttcgcga
    10141 tgggtgataa tgacgccacg gggaaaacgg gtgctgccgg aggtgtactg gaggtaggcg
    10201 atatcgttcg gaactggacg ctggagcgca acatcggctt ccggtaatgc cttaaaccag
    10261 gcgtggctta aaacatgtaa ttcggggttg tcatgcgtcg cggcattgaa cagtggcaac
    10321 cactcatcac cagtgataat ggctgcgggc tggcaacttg ccagtaaacc ctgcaatttg
    10381 gcgctccagg aatcccgctg accaacgccc attggaatcg ccaacgggac ggcgactaag
    10441 ccggcatact ggcaggcaaa aaaagcctct acgaacccgc tacttgtttc ggcaatcagt
    10501 gccacgcgat cgcctttttt caggttcagc gataacaacc gcttcgcacc agcttcggca
    10561 cgtgctttta acgtctgata ttccagttga tcttcaagtt ggcaacgtct gtcataaaaa
    10621 ttcattccgg cgctactcag agcggcgtag tccaaagcat caaccagcgt tggaaaatcg
    10681 gcatagcgca taggtaggga atgcgtaaag attttattag acatatacac cacgaaaaaa
    10741 ttggata
//
LOCUS       DAPHBJ010000153        10618 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.155, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000153 DAPHBJ010000000
VERSION     DAPHBJ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.155"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            180..1547
                     /gene="dcuC"
                     /locus_tag="RHZ76_003432"
     CDS             180..1547
                     /gene="dcuC"
                     /locus_tag="RHZ76_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDV2814020.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPTKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1590..2087)
                     /gene="sspB"
                     /locus_tag="RHZ76_003433"
     CDS             complement(1590..2087)
                     /gene="sspB"
                     /locus_tag="RHZ76_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HDV2814021.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2093..2731)
                     /gene="sspA"
                     /locus_tag="RHZ76_003434"
     CDS             complement(2093..2731)
                     /gene="sspA"
                     /locus_tag="RHZ76_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HDV2814022.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3126..3518)
                     /gene="rpsI"
                     /locus_tag="RHZ76_003435"
     CDS             complement(3126..3518)
                     /gene="rpsI"
                     /locus_tag="RHZ76_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDV2814023.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3534..3962)
                     /gene="rplM"
                     /locus_tag="RHZ76_003436"
     CDS             complement(3534..3962)
                     /gene="rplM"
                     /locus_tag="RHZ76_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDV2814024.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4181..5308)
                     /gene="zapE"
                     /locus_tag="RHZ76_003437"
     CDS             complement(4181..5308)
                     /gene="zapE"
                     /locus_tag="RHZ76_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HDV2814025.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYAIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5502..5900
                     /locus_tag="RHZ76_003438"
     CDS             5502..5900
                     /locus_tag="RHZ76_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HDV2814026.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            6054..7421
                     /gene="degQ"
                     /locus_tag="RHZ76_003439"
     CDS             6054..7421
                     /gene="degQ"
                     /locus_tag="RHZ76_003439"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HDV2814027.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7511..8578
                     /gene="degS"
                     /locus_tag="RHZ76_003440"
     CDS             7511..8578
                     /gene="degS"
                     /locus_tag="RHZ76_003440"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HDV2814028.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8641..9579)
                     /gene="mdh"
                     /locus_tag="RHZ76_003441"
     CDS             complement(8641..9579)
                     /gene="mdh"
                     /locus_tag="RHZ76_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDV2814029.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            10014..10484
                     /gene="argR"
                     /locus_tag="RHZ76_003442"
     CDS             10014..10484
                     /gene="argR"
                     /locus_tag="RHZ76_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDV2814030.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2610 a         2694 c         2646 g         2668 t
ORIGIN      
        1 attatgaatg tgttcttaca aaataatcat aagcgcatgt tttttaatga aaaatcacct
       61 cacctacaat taaaaacacg acatccgcac cataaatagc cttgcaaaaa atataacatc
      121 gttgttttca atctgccgtt tatgggattg accgttttct tttgacacgg agttcaacaa
      181 tgttcggcat aattatatct gtcatcgtat taattacgat gggctatttg atcctgaaaa
      241 actacaaacc tcaggtggtg ctggctgccg caggtatctt cctgatgatg tgcggtgtct
      301 ggttagggtt cggtggtgta ctcgatccca ccaaaagcag cggctacttg atcgtcgata
      361 tttataatga aatcctgcgc atgctgtcca accgcattgc cggattgggg ctgtcgatta
      421 tggcggtggg cggttatgcc cgctacatgg agcgcatagg ggccagtcgc gcgatggtga
      481 gcttgttaag ccgcccgtta aaactcattc gctcgccgta tattattctg tcggcaactt
      541 acgtcatcgg ccaaatcatg gcgcagttta ttaccagcgc ctccggtctg ggtatgttgc
      601 tgatggtcac cttatttccg acgctggtga gtctgggagt aagtcgcctc tctgcggtgg
      661 cggttatcgc aaccacaatg tccattgagt ggggaattct ggaaacgaac tccatttttg
      721 ctgcccaggt agcgggaatg aaaattgcca catacttctt ccactaccag cttccggtcg
      781 cctcttgcgt cattatctcg gtggcgatct cccacttttt cgtgcaacgc gcttttgaca
      841 aaaaagataa aaatatcaat cacgaacagg cagagcaaaa agctctcgat aatgtcccgc
      901 cgctctatta cgccatttta cctgtgatgc cgttaatcct gatgctcggc tcgctgttcc
      961 tcgcccacgt cgggctgatg cagtcagaac tgcatctggt ggtggtgatg ttactgagtt
     1021 tgacggtgac gatgtttgtt gagttcttcc gcaagcataa cttgcgcgaa acaatggacg
     1081 atgtgcaggc gttttttgac ggcatgggta cgcagtttgc caacgtggta acgctggtgg
     1141 tcgcgggtga aatatttgcg aaaggcttaa cgacgattgg cactgtcgat gcggttatca
     1201 ggggtgcgga gcattctggt ctgggcggta ttggcgtgat gattattatg gcgctggtca
     1261 ttgccatttg tgccattgtg atgggctctg gcaatgcgcc gtttatgtca tttgccagtc
     1321 ttattccgaa tatcgcagcc ggactacatg taccagcggt tgtaatgatt atgccgatgc
     1381 attttgccac gacgctagcg cgcgcggttt cgccgattac tgcggtggtg gtcgttacgt
     1441 caggaattgc aggcgtttcg ccttttgcgg tggtgaagcg aacagcgatc cccatggcag
     1501 tcggtttcgt ggtgaatatg attgccacaa tcacgctatt ttattaagcc attaaaagac
     1561 aaaacaggcc accagggcct gtttttgtat tacttcacaa cgcgtaatgc cggtcgacca
     1621 ccgcgtggtg gctgcggagg ttcatcgtca ggatgagtgt catcatcgtg atctggcttg
     1681 tcgccatcaa taaccgacat aacggtttcg ttgtctgccg atgcctcttc atcattcatg
     1741 atgctggtat cttcatcgta ggcagcttca ggctcaaaca tcgtgcctgc gccattttca
     1801 cgggcgtaga tagccagcac ggcagccagc ggcacagaaa cctgacgcgg aatgccacca
     1861 aagcgcgcgt taaagcgtac ctcatcattc gccagttcca gattgccgac agcacgcggc
     1921 gcaatgttga gtacgatttg cccgtcacgc gcatattcca taggaacctg cacgccaggg
     1981 agcgtcacat ccaccaccag gtgcggcgtg agctggttat ccagcaacca ctcatagaat
     2041 gcacgcagca gatagggacg acgtggtgtt agctgtgaca aatccataca gattaactcc
     2101 ggcccagacg catttcacgt tctgcttcag ttaaagaagc aaggaaagag tcacgctcaa
     2161 agacgcgggt catatagcct ttcagctctt tcgcacccgg gccgctgaac tcgatgccca
     2221 gttgcggcag acgccacagc agcggagcaa gatagcaatc gaccaggctg aactcatcgc
     2281 tcaggaagta cggcttctga ccgaagaccg gcgcaatcgc cagcagttct tcgcgcagtt
     2341 gcttacgtgc ggcatctgct tcagaagctg aaccgttgat gatggtgttc atcagcgtgt
     2401 accagtcttt ttcgatgcga tgcatgtaca gacggctttc accgcgagct accgggtaaa
     2461 caggcatcag tggcggatgc gggaaacgct catccagata ttccataatg atgcgagatt
     2521 cccacagggt cagctcacga tccaccaggg tcggaacgct ctgattcggg ttgaggtcaa
     2581 tcagatcctg aggcggattg tccttttcca cgtgttcgat ctcgaaactt acacctttct
     2641 cagccagcac aatgcggacc tgatggctat agatgtcagt aggaccggaa aacagcgtca
     2701 ttaccgaacg tttgttggca gcgacagcca tgaaaacctc caggtatagt cagaattttt
     2761 actgctacca gccaccaggt ggccagtcag aagttgtgtt acccaataag gaacgactct
     2821 ctttgttcga aaatcaaaca aaaaatgagc aatacccgac atttgggcag aaaattggat
     2881 gatagtttac cagattttgc gaccattgtg gtgagtcgat gccggaaatg gggaaaaaga
     2941 gatgcgcttt agtctgaaat agttgactta gtcccttatt ggcgatgtgg tttttgtttt
     3001 acctgtctgt caggtggcag caaaaagcaa ctttccagtt tttacgctga ttcagatttt
     3061 agctataaaa aaacccgccg aagcgggttt tttcgaaaat tgttttctgc cggagcagaa
     3121 gccaattaac gtttggagaa ctgcggacga cgacgtgctt tacgcagacc gactttctta
     3181 cgttcaacct gacgagcgtc acgagtaacg aagccagctt tacgcagttc agaacgcagg
     3241 gactcgtcgt attccatcag agcgcgggtg ataccgtgac ggatcgcacc agcctgacca
     3301 gagataccac cacctttaac ggtgatgtac aggtccagtt tctcaaccat gtcgaccagt
     3361 tccagcggct gacgaactac catgcgggca gtttcacgac cgaagtactg ttccagagaa
     3421 cgttggttga ttacgatttt accgttgccc ggtttgatga aaacgcgagc tgcggaactt
     3481 ttgcggcgac cagtgccgta gtattgattt tcagccattg cctataatcc cgattagatg
     3541 tcaagaactt gcggttgctg tgccgcgtgg ttgtgctcgt tacccgcgta aactttcagt
     3601 ttacggaaca tagcacgacc cagcgggcct tttggcaaca tgcctttaac cgcgatttca
     3661 atcacacgct caggacggcg agcaatcatc tcttcaaagg tcgcttgttt gataccaccg
     3721 atgtggccgg tgtggtgata gtacactttg tcagtacgct tgttgccggt tacagcaact
     3781 ttgtcagcgt tcagaacgat gatgtaatca ccggtatcta cgtgcggagt gtattccgct
     3841 ttgtgcttac cgcgcaggcg acgagccagt tcagtagcca gacggcccag agttttaccg
     3901 gtcgcgtcaa caacatacca gtcgcgtttt acggtttctg gtttagctgt aaaagttttc
     3961 attaaaagct tacccaataa atagttacac gttggtgaac acccaaacgt cttcaattgt
     4021 tgaggttcac acgacaaagt ccggcaaacc taccccttcg aatagcctat gccagcacac
     4081 aaaaagtttt gggaaaaaaa ctttcttgta acgtggggtc gcaggattat agagaagtcg
     4141 gggtcaaaga tcgacccctt tttgtgattt gtggcaggtt ttaacctgct aaatgctcgc
     4201 gcttcagata ctcttcgctt tgcatctctt gcagacgtga caggcaacgc tggaactcaa
     4261 atttcagccg ctcgccctga taaattgcat acagcggcac ttctgcactc accactaatt
     4321 tgacatggcg ctcgtaaaac tcatccacca gcgcaataaa gcgccgcgct tcgctctcca
     4381 tcaaccgcgt cataactggt acatcaaaca acatgaccgt atgaaagaga cgtgagagcg
     4441 caatatagtc atgctgactg cgggcgtcga cgcacagcgt agtaaaagag accgccagcg
     4501 tctggttctc gacgcccatt gtcgccaatg gccgatggtt gatttctaac gtcggtgaat
     4561 tttctcgttt cgcccccgcc agcgccaacc atagtttatc catctgcgcc cgggtttcat
     4621 cgttgagtgg cgaaagccac agatgcgcct gagtgagtgt acgcagtcga taatcaacac
     4681 cagcgtccac gttcattaca tcacaatgct gtttaatggc atcgattgca ggcagaaaac
     4741 gcgcacgttg caggccattt cgataaagtt cgtccggcgg aatatttgac gtcgctacca
     4801 gggtaatacc gcgggtgaac agggctttca tcagaccgcc aagtagcatg gcgtcggtaa
     4861 tatcagaaac aaaaaattcg tcaaaacaga gcacgtcagt ttcggcttta aagcgatcgg
     4921 caataatttc cagcggatcg gtctgcccct gtaaggcagt tagctcttcg tgcacccgca
     4981 gcataaaacg gtgaaagtgc aggcgctgtt tccgctctcc cggcaggctt tgatagaaaa
     5041 ggtccatcag ccaggttttc ccgcgtccta caccgcccca catatataag ccacgcactg
     5101 gcgtatgctt tgtgtcttcg cgtttacccc acagcttacc gacccgcgcc attagcccac
     5161 tcgtcctggg ggctggtggc ctgctattga tgagttcttg ataaataatt tccaggcggc
     5221 tgacggcctc tttttgaacg tcgtcgggtt gatggctgcc ttcattgagc gccttcaggt
     5281 attgcgatgt tggggtaacg ctttgcatga tcttattgtt attccttgaa taatcggtgc
     5341 gccgttgttc acggttgacg aaaaaaaggc cgttctacac tacgcgatat gcagtcggga
     5401 ttccacttct gtggaattaa cggttatagt ggcataatca gccgcaggca tggagcctga
     5461 agccaacacc ctacggaaac aaaagacaac gggagatgtt catgacctgg gaatatgcgc
     5521 taattgggtt agtcgtcggc atcattattg gtgctgtggc catgcgtttt ggtaatcgta
     5581 aactacgcca gcaacaggcg ttgcagtacg aactggaaaa gaataaagct gaactggacg
     5641 agtatcgcga agagctggtt agccactttg cccgcagcgc ggaattactg gataccatgg
     5701 cgcacgacta tcgccagctg tatcagcaca tggcaaaaag ctccagcagc ctgctgccgg
     5761 aactgtctgc tgaagcaaac ccgttccgta atcgtctggc agagtctgaa gccagcaacg
     5821 atcaggcacc ggtgcagatg cctcgcgact attctgaagg cgcatccggc ctgctgcgta
     5881 ctggcgcgaa gcgcgactaa tttatttttc gggcgcagcc attgcgccct cctcttctct
     5941 ccctccccga ctatcattta atctggtgtc tcattgttag ccgtctgaaa attcaataac
     6001 atcaaactgt tttgaatctc ttttcttatc attcaggtac gagagcagga ataatgaaaa
     6061 aacaaaccca gctgttgagt gcattagcgt taagtgtcgg gttaactctc tcggcgtcat
     6121 ttcaggccgt cgcgtcgatt ccaggccagg ttgccgatca ggcccctctc cccagtctgg
     6181 ctccaatgct ggaaaaagtg cttccggcag tggtgagcgt acgggtggaa ggaacggcca
     6241 gtcagggaca gaaaatcccg gaagaattca aaaagttttt tggtgatgat ttaccggatc
     6301 aacctgcaca acccttcgaa ggtttaggct ccggtgtcat catcaacgcc agtaaaggct
     6361 atgtgctgac caacaaccat gtgattaatc aggcacagaa aatcagtatt cagctcaatg
     6421 atgggcgcga gtttgatgca aaactgattg gtagcgatga ccagagcgat atcgccctgt
     6481 tacaaattca aaacccgagc aaattaacgc aaatcgctat tgccgactcc gataaattgc
     6541 gcgtcggtga ttttgccgta gcggtcggta acccatttgg ccttgggcaa accgccacct
     6601 ctggcattgt ttccgcatta ggccgcagcg ggttgaatct tgaaggtctg gaaaacttta
     6661 tccagacaga tgcttccatt aaccgcggta actccggcgg tgcactatta aaccttaacg
     6721 gtgagttaat tggcatcaac actgcaatcc ttgcgcctgg cggcgggagc gtcgggattg
     6781 gatttgccat ccccagtaat atggcgcgaa cactggcgca gcagcttatc gactttggtg
     6841 aaatcaaacg cggtttgtta ggcatcaaag gcaccgagat gagtgccgat atcgccaaag
     6901 ccttcaacct tgacgtgcag cgtggcgcgt ttgtcagcga agtgttgcca ggttctggct
     6961 cggcaaaagc gggcgtcaaa gcgggcgata ttattaccag cctcaacggc aaaccgctga
     7021 atagctttgc tgagttgcgc tctcgtatcg cgaccaccga gccgggcacg aaagtgaagc
     7081 ttggcctgct gcgtaacggc aaaccactgg aagtagaagt gacgctcgat accagcacct
     7141 cttcgtcggc cagcgctgaa atgatcacgc cagcgctgga aggtgcaacg ttgagcgatg
     7201 gtcagctaaa agatggcggc aaaggtatta aaatcgatga agttgtcaaa ggaagcccag
     7261 ctgctcaggc tggcttgcaa aaagacgatg tgatcattgg cgtcaaccgc gatcgggtga
     7321 actcgattgc tgaaatgcgt aaagtgctgg cggcaaaacc ggccatcatc gccctgcaaa
     7381 ttgtacgcgg caatgaaagc atctatctgc tgatgcgtta atgtcgtaaa ccgggcatca
     7441 ggcttacgtg tgatgtccgg ttaactcgtg gtatgctgct gccgttccct tttttaatga
     7501 cgcctccatc atgtttgtga agctcttacg ttccgttgcg attggattaa ttgtcggcgc
     7561 tattctgctg gttgccatgc cttcgctgcg cagccttaac ccgctttcca ctccgcaatt
     7621 tgacagtacc gatgagacgc ctgccagcta taatctggcg gttcgccgcg ccgcgccagc
     7681 ggtggttaac gtttacaacc gtggtttgaa caccaactct cacaaccagc ttgagatccg
     7741 caccctggga tccggtgtaa tcatggatca acgcggttat atcatcacca ataaacacgt
     7801 catcaacgac gccgatcaga tcatcgtcgc cttacaggat ggacgtgtat ttgaagcatt
     7861 gctggtggga tctgactctc taaccgatct ggcggtactt aaaattaatg ccactggcgg
     7921 tttacctacc attccaatta atgcacgtcg cgtaccgcac attggcgacg tagtactggc
     7981 gatcggtaac ccgtacaacc tcgggcagac cattacccaa gggattatta gtgccacggg
     8041 tcgaatcggt ctgaacccga ccgggcggca aaacttcctc caaaccgatg cttccattaa
     8101 ccacggtaac tctggcggcg cgctggtgaa ctcgctgggc gaactgatgg gcattaatac
     8161 gctgtcgttt gataagagta acgatggcga aacgccggaa ggtatcggct ttgcgattcc
     8221 tttccagtta gcaaccaaaa ttatggataa gctgatccgc gatggtcgcg tgatccgcgg
     8281 ctacattggt atcggcggac gtgagatcgc accactgcac gcgcagggcg gtggtataga
     8341 tcaactgcaa gggatcgtgg ttaatgaagt gtcacctgac ggcccggcgg cgaatgcggg
     8401 tattcaggtc aacgatctga ttatttcggt ggataacaaa ccggccatct ctgctctgga
     8461 gacgatggat caggtggcgg aaattcgccc tggttcggtg atccctgtag tagtgatgcg
     8521 tgatgataag cagttaacgc tgcaggtcac cattcaggaa tatccggcaa ccaattaagt
     8581 cgtgcgctca aaacaaaaaa ccggagtctg tgctccggtt ttttattatc cgctaatcaa
     8641 ttacttatta acgaactctt cgcccagggc gatatctttc ttcagcgtat ccagcatacc
     8701 ttccagcgcg ttctgttcaa atgcgctcag ggtaccgata gatttacgct cttccacgcc
     8761 gtttttaccc agcagcagcg gttgagagaa gaaacgggcg tactgaccgt cgccttcaac
     8821 gtaggcacat tcgacaacgc cttgttcgcc ctgcagtgca cgaaccagag acagaccaaa
     8881 acgtgcagct gcctggccca tagacagggt tgcagacccg ccaccggcct tcgcttcaac
     8941 cacttcagta cccgcgttct ggatgcgttt ggtcagatca gccacttcct gctcggtaaa
     9001 actaacgcca ggaacctgtg acagcagcgg cagaatggta acaccagagt gaccgccaat
     9061 aaccggcact tcaacttcgc ctggctgttt gcctttcagt tccgcaacaa aggtgttgga
     9121 acgaatgata tccagcgtgg taacgccgaa cagtttgttt ttgtcataaa caccggcttt
     9181 tttcagcact tcagcagcaa ttgcaactgt ggtgttaacc gggttagtga taataccaat
     9241 gcacgctttc gggcaggttt tcgcaacttg ctgtaccagg tttttcacga tgccggcgtt
     9301 aacgttaaac aggtcggaac gatccatacc cggtttacgc gctacgcctg cagagataag
     9361 aacgacatct gcgccttcca gcgccggagt cgcatcttca ccagaaaaac ctttgatttt
     9421 cacagcagta gggatatggc tcagatcgac agccacaccg ggagtcactg gagcgatatc
     9481 atacagagag agttctgaac ctgaaggcag ttgggttttt aacagtagtg caagcgcctg
     9541 gccaataccg ccagcagcgc cgaggactgc gactttcatc ctaaactcct tattatattg
     9601 ataaactaag atatgttgct ccgctgccgc gaccttaatc cacaaaattg ccgtttacaa
     9661 tgaccacatc tcaagaatgt gtagtcacgc aagtttagcg tttatgcatt taattgccgt
     9721 aatcaggaac ctaacttacg taattaacag tcgcttatgc attagcgcaa cattccaaca
     9781 ggtggtgaca atatacccta ccgttcagcc aaaacaacat caatttgata acaattaatt
     9841 tacttttaag cagaatttgc atgccgtgac gcaggcatgt ttctcaataa cgaaatttga
     9901 taaaatcccg ctctttcata acattatttc agccttcttc agggctgact gtttgcataa
     9961 aaattcatct gtatgcacaa taatgttgta tcaaccacca tatcgggtga cttatgcgaa
    10021 gctcggctaa gcaagaagaa ctagttaaag catttaaagc attacttaaa gaagagaaat
    10081 ttagctccca gggcgaaatc gtcgccgcgt tgcaggagca aggctttgac aatattaatc
    10141 agtctaaagt ctcgcggatg ttgaccaagt ttggtgctgt acgtacacgc aatgccaaaa
    10201 tggaaatggt ttactgcctg ccagctgaac tgggtgtacc aaccacctcc agtccattga
    10261 agaatctggt gctggatatc gactacaacg atgcagttgt cgtgattcat accagccctg
    10321 gcgcggcgca gttaattgct cgcctgctgg actcactggg caaagcagaa ggtattctgg
    10381 gcaccatcgc tggcgatgac accatcttta ccacccctgc taacggtttc acagtcaaag
    10441 acctgtacga agcgatttta gagctgttcg accaggagct ttaatctctg ccccgtcgtt
    10501 tctgacggcg gggaaaatgt tgcttatccc tctcaacccc ctgctttccc ctgcgattaa
    10561 tttaacgaat agtgcgtttt actgcgacat gtcattcaca caatgaatac ataaggta
//
LOCUS       DAPHBJ010000154        10597 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.156, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000154 DAPHBJ010000000
VERSION     DAPHBJ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.156"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            31..597
                     /locus_tag="RHZ76_003443"
     CDS             31..597
                     /locus_tag="RHZ76_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HDV2814031.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            601..1176
                     /locus_tag="RHZ76_003444"
     CDS             601..1176
                     /locus_tag="RHZ76_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HDV2814032.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQDVDLIISVEGVQQK"
     gene            complement(1218..2270)
                     /locus_tag="RHZ76_003445"
     CDS             complement(1218..2270)
                     /locus_tag="RHZ76_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDV2814033.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDSALDGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2495..3415
                     /gene="lpxL"
                     /locus_tag="RHZ76_003446"
     CDS             2495..3415
                     /gene="lpxL"
                     /locus_tag="RHZ76_003446"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HDV2814034.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3587..4813
                     /gene="mdtG"
                     /locus_tag="RHZ76_003447"
     CDS             3587..4813
                     /gene="mdtG"
                     /locus_tag="RHZ76_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HDV2814035.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4896..5270
                     /locus_tag="RHZ76_003448"
     CDS             4896..5270
                     /locus_tag="RHZ76_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDV2814036.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5271..5498)
                     /locus_tag="RHZ76_003449"
     CDS             complement(5271..5498)
                     /locus_tag="RHZ76_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDV2814037.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5671..8214)
                     /gene="mdoH"
                     /locus_tag="RHZ76_003450"
     CDS             complement(5671..8214)
                     /gene="mdoH"
                     /locus_tag="RHZ76_003450"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HDV2814038.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8207..9742)
                     /gene="mdoG"
                     /locus_tag="RHZ76_003451"
     CDS             complement(8207..9742)
                     /gene="mdoG"
                     /locus_tag="RHZ76_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HDV2814039.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10136..>10597
                     /locus_tag="RHZ76_003452"
     CDS             10136..>10597
                     /locus_tag="RHZ76_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDV2814040.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCV"
BASE COUNT         2600 a         2769 c         2616 g         2612 t
ORIGIN      
        1 ttctctcgca aattcgtttt ggatgcactt atgtcattca caaatacccc tgaacgctat
       61 ggtgttatat ctgcggcctt ccattggcta agcgcaatta ttgtctacgg catgtttgct
      121 ttaggtctgt ggatggtcac gctcagttat tacgatggct ggtatcacaa agcacccgaa
      181 ctgcataaaa gcatcggtat tttgttaatg atggggctgg ttattcgcgt tctgtggcgt
      241 gtcatatctc caccgccggg tccgctgccg agttattcgc ccatgactcg tcttgccgca
      301 aaagctggac atctcgcatt gtacctattg ctgttcgcaa tcggcatcag cggctatctg
      361 atctcaactg ccgatggtaa accgatcagc gtttttggct ggtttgacgt ccccgcgacc
      421 cttgccgacg ctggcgcaca ggcagacttt gccggtgccc tgcatttttg gctcgcgtgg
      481 agcgtcgtag tactgtccgt tatgcacgga tttatggccc tgaagcatca tttcatcgat
      541 aaagacgaca ctctgaagcg catgctggga aagtcgtcat ctgactatgg agtataaaaa
      601 atgaaaaaaa gcctgcttgg tttaaccttc gcgtccctga tgttctctgc cggttcagcg
      661 gttgccgccg attacaaaat tgacaaagaa ggtcagcacg cctttgttaa tttccgcatc
      721 cagcaccttg gctatagctg gttatacggc acctttaaag atttcgacgg tacttttacc
      781 tttgacgaaa aaaatccggc tgccgataaa gtgaatgtga caattaacac caccagcgtc
      841 gatactaatc acgccgaacg cgataaacat cttcgcagtg cagatttcct caataccgca
      901 aaatatccac aggcaacatt cacctccacc agcgtgaaga aagacggtga cgaactggat
      961 attaccggcg atctgacact gaatggcgta accaaacccg tcacgctgga agcgaaatta
     1021 attggtcagg gcgacgaccc gtggggcggt aaacgtgcag gctttgaggc cgaaggcaaa
     1081 attaagctca aagacttcaa tatcaagaca gatttaggtc cagcttctca ggatgtggat
     1141 ctgattattt cagtggaagg cgtacagcag aagtaagcgt gaaatgttgc cggatggcac
     1201 gcatccggca atgatattta ttctgttgga tcaggaatgc ccagtgttgt attcagacgt
     1261 ccacgagact tattaaagat cttattgcca ttttcacgtc ctgcccgacg gcgtcgctgt
     1321 tcttctggcg gtaacgcgct ttcttcgcag caaatctcac tacaacaacc tttgtatttt
     1381 tccgcgcata ctggacactg aataaacagc aggtggcagc catcattttt acagttggta
     1441 tggctgtcgc acggcgcgcc gcactggtgg caatgcgcga taatctcatc cgatatacgt
     1501 tcgcccatcc gctcgtcaaa aacaaaattt ttgccaataa aacgcaccgg caagccctgc
     1561 tcgcgcgcct tacgggcgta ttcaataatt ccgccctcga tatgccagac tttgttgaat
     1621 ccgttatgtt tcatccaggc actggccttt tcacaacgaa tgccgccggt gcagtacatg
     1681 acgatttttt tatctttatg tgcctgcatc atctcgactg cttttggcag ctgctcacgg
     1741 aaggtatctg ccggaatttc gagcgcgttt tcaaagtgcc ccacttcata ctcatagtgg
     1801 ttacgcatgt cgataaacag tgcatcggga tcgtcaagca tggcgttcac ttccgccgct
     1861 tgcagatact caccaacatt gctggcatca aagtgaggat cgtcaatacc gtcggcaacg
     1921 atacgatcgc ggaccttcat gcgcagtacc cagaaggatt tcccgtcatc atccaacgcg
     1981 atattcaggc gtaagccatc taaagccgag tcgaaggcat agagctgcgc gcgaaatgtt
     2041 tcaacattgc tcgcaggtac gctgatttgc gcgttaatgc cctcatgcgc cagatacact
     2101 cgcccaaaaa cattcagcgc ggtaaacagc tgatataaag cgtcacgggt cgccttagga
     2161 tcggcgatgt ggaaatactt gtaaaacgaa atggtggttc gcggttcgct ctcagccaac
     2221 attttggctt ttagcgcgtc gttggaaatg cggttgtgta acactggcat ggtgtacggt
     2281 tcctgcgaga tgggaaagta aaaatccgcg gcatgatata gcaattatcg ataattaaca
     2341 tccacacatt ttacgctaca tttgcgcatt aaaaattatt tgttatttac aagcgcggca
     2401 atttcgccca gtcttcagcc acaaattttg gttgcgggcg aaaaaatgcg acaatacata
     2461 caattgcccg aataggttga aaaacaggat tgatatgacg aatctaccca agttctccac
     2521 cgcactgctt catccgcgtt attggttaac ctggttgggt attggcgtac tttggttagt
     2581 cgtgcaattg ccctacccgg ttatctaccg cctcggttgt ggattaggaa aactggcgtt
     2641 acgttttatg aaacgacgcg caaaaattgt gcatcgcaac ctggaactgt gcttcccgga
     2701 aatgagcgaa caagaacgcc gtaaaatggt ggtgaagaat ttcgaatccg ttggcatggg
     2761 cctgatggaa accggcatgg cgtggttctg gccggaccgc cgaatcgccc gctggacgga
     2821 agtgatcggc atggaacaca ttcgtgacgt gcaggcgcaa aaacgcggca tcctgttagt
     2881 tggcatccat tttctgacac tggagctggg tgcgcggcag tttggtatgc aggaaccggg
     2941 gattggcgtt taccgcccga acgataatcc actgattgac tggctacaaa cctggggccg
     3001 tttgcgctca aataaatcga tgctcgaccg caaagattta aaaggcatga ttaaagccct
     3061 gaaaaaaggc gaagtggtct ggtacgcacc ggatcatgat tacggcccgc gctcaagcgt
     3121 tttcgtcccg ttgtttgccg ttgagcaggc tgcgaccacg accggaacct ggatgctggc
     3181 acggatgtcc ggcgcatgtc tggtgccctt cgttccacgc cgtaagccag atggcaaagg
     3241 gtatcaattg attatgctgc cgccagagtg ttctccgcca ctggatgatg ccgaaactac
     3301 cgccgcgtgg atgaacaaag tggtcgaaaa atgcatcatg atggcaccag agcagtatat
     3361 gtggttacac cgtcgcttta aaacacgccc ggaaggcgtt ccttcacgct attaaatctc
     3421 ccatgccgga tgcttcagaa tggcatccgg cattaccaca gcaaatcccc ctgatttagc
     3481 gataaaagct ctctggattg cgccccctgg aagtcgggcg cataattagt gtgcttatct
     3541 tttcttctta tgttcaccgc gcctggcgca ccaacagcgg attgctatgt caccctgtga
     3601 aaatgacacc cctataaact ggaaacgaaa cctgatcgtc gcctggctag gctgttttct
     3661 taccggggcc gccttcagtc tggtaatgcc cttcttaccc ctctacgttg agcagcttgg
     3721 cgttaccggt cactccgccc tgaatatgtg gtccggtatt gtcttcagca ttacattttt
     3781 attttcggcc atcgcctcac cgttttgggg tggactcgcc gaccgtaaag gccgaaaact
     3841 catgctatta cgctctgctc tcggcatggg catcgtaatg gtgttgatgg gactggcaca
     3901 aaatatctgg cagtttttga tcctacgggc gcttcttggg ttacttggcg gatttgtccc
     3961 caacgctaat gctcttatcg ccacacaagt accgcgtaat aaaagcggct gggcgctggg
     4021 tacgctctcc acaggcggcg ttagtggtgc gttgctcggc ccaatggctg gcggcctgct
     4081 cgccgatagc tacggcttac gtccggtatt ctttattacc gccagtgtgc tcatactctg
     4141 ctttttcgtc accctgtttt gcatcagaga aaaattccag ccggtcagca aaaaagagat
     4201 gctgcacatg cgggaagtgg tgacatcact taaaaacccg aaactggtac tcagcctgtt
     4261 tgtcactacg ttaatcatcc aggtggcgac gggctcaatt gcccccattc tgacgctgta
     4321 tgtccgcgaa ctggcgggta acgtcagtaa cgtcgccttt atcagtggca tgatcgcctc
     4381 ggtgccaggc gtggcggctc tgctaagtgc accacgactc ggcaaacttg gcgatcgaat
     4441 cggacccgaa aagatcctga ttacagcgct gatcttttct gtactgctgt tgatcccaat
     4501 gtcttacgtt cagacgccat tgcaacttgg gattttacgt tttttgctcg gtgccgccga
     4561 tggtgcacta ctccccgccg tacagacact gttggtttac aactcgagca accagatcgc
     4621 cgggcgtatc ttcagctata accaatcgtt tcgtgatatt ggcaacgtta ccggaccatt
     4681 gatgggagca gcgatttcag cgaactacgg tttcagagcg gtatttctcg tcaccgctgg
     4741 cgtagtgtta ttcaacgcag tctattcatg gaacagtcta cgtcgtcgtc gaatacccca
     4801 ggtatcgaac tgatttttcg cctttcatac ttgcaaaagc ggagaatcag ctatcctttt
     4861 ccctgaaacc tcatcaactc aaagggagaa tcgtgatgac catgtacgca acgcttgaag
     4921 aagccattga cgctgcacgc gaagaatttc ttgcagacaa ccccggcatc gacgccgaag
     4981 atgcgaatgt gcaacagttc aatgcccaaa aatacgtttt gcaggacggc gacatcatgt
     5041 ggcaagttga gttttttgcc gacgaagggg aagaaggtga atgtttacct atgcttagcg
     5101 gtgaagccgc gcaaagtgtt tttgatggcg actatgatga gatagagata cgccaggagt
     5161 ggcaggaaga gaatacatta catgaatggg acgaggggga atttcagctt gagccaccgc
     5221 tggataccga ggaaggacgc gcagcagctg atgagtggga tgaacgttaa tcactcatac
     5281 gggccatgat gaatgtcgat cggcagcagt aaagtatcaa aaaccaggga gaatggcaga
     5341 tcaaggattg tgacataacg ccaggcggag tcacgcacat cccattgcac accgggataa
     5401 tattggttgc catgcccctg ccccggtata gtgcgactaa taatgctgcc acagccactc
     5461 agcaaggtca ccatgatgct caccacaatt aaacgcattt gatacctctt ccgtaaaaaa
     5521 ataccggcat aacgccggta tttttattgg ctcgttgttt ttgccggatg cggcgtgaac
     5581 gccttaccca acctacaaaa tcttgcaaaa tcaataaatt gcaggaacga tgtaggcctg
     5641 ataagcgtag cgcatcaggc aactacgttt ttattgcgaa gccgcatccg gtttacgcaa
     5701 tgccagtgga ttgagcttta tcccttcgta ataactcacc catgaagaat atctctccgg
     5761 ggaattccag acgcggaaat gcagacgggc catcgtcacc ggatcgctta gcagcaccag
     5821 gcgacgatcg cgattcagct tctctggcgt ctcgttaagc gcctgttcaa cgtggcggtc
     5881 acgggcgatt tccagcacct tactggcgcg gtgacgcgcg gtcgccattg cggttgccag
     5941 agcgttaaat gacgggttaa acaccgcgtg cataaaacca tcatcaaggg agcgttgacg
     6001 attcatctca aggaagcgat cggtatcaac cagcacctgc ggcggtgaat actcttccgg
     6061 gatcaggaac agtttccagc gtttggttcg cagaccaacg gtggcacggc tggaaatcac
     6121 cgaaacaaac ggtgacagga tcaacgagaa gacaatcggt gccagccaga acaggaaacg
     6181 cagatccaac cacgccatac caacagccca cactaaccct aacagcagct gtgagccgtg
     6241 gcgtttgaac gcttcacccc aggaagtgga gtcatcatca cgctgcggtg aattccacac
     6301 aacttcccag ccaaggaacg cgctgacgac gaagaccgta tggaacagca tgcgtaccgg
     6361 agccagcagc acggaaaaca gcacttccag cagcaacgat aatgtaacgc gccagaatcc
     6421 gccgtattct ttcgttcctt tgcaccagat aagcaaaatg ctcaataact tcggcaggaa
     6481 caacagcacc atagtcgaag caaaaagtgc aatcgccagc tcaggacgcc actgcggcca
     6541 caccgggaac aactgccgtg gttgcaggaa gtattgcggt tcggtcaacg catgtactac
     6601 ctgcaatgca gtagagagcg cgaggaacat aaaccacagc ggagcggaga gataagacat
     6661 cacgcccgtc aggaacaccg cacggtgaac cgggtgcata cccttcacca ggaacagacg
     6721 gaagttcatc aggttaccgt ggcaccagcg gcggtcacgt tttagctcat caagcaagtt
     6781 aggcggcaat tcttcataag aacccgggag atcgtaagca atccagaccc cccaacctgc
     6841 acggcgcatc aacgccgctt ccacgaagtc atgtgacagg attgaaccgg caaaggaacc
     6901 ttcgcccggc agcggagcca gtgcgcagtg ctcgataaac ggtttcacgc ggataatcgc
     6961 gttatgtccc cagtagtgcg actcgccaag ttgccagaag tgcaaaccgg ctgtaaacag
     7021 tggcccatac acgcgggtcg cgaactgctg acagcgcgca tacagcgtat ccataccgga
     7081 cgctttcggc gacgactgaa tgatcccggc gttcgggttg gcttccatca ggcgcaccag
     7141 cccgcacaaa caatcaccgg tcattaccga gtcagcatcc agcaccacca tgtagctgta
     7201 ctggctgccc cagcgacggc agaagtcatc gatattaccg cttttacgct tcacgcggcg
     7261 acggcggcgg cgatagaaaa tctgaccttc gccaccgact tcagcgataa gctccatcca
     7321 ggctttttgc tctgcgacgc agatatccgg gttataactg tcactaagaa tgtagacatc
     7381 aaagtgtttg gcattcccgg tggcttttac tgattcccac gttgcacgca ggccagcaaa
     7441 aacacggttc acgtcttcgt tacagatagg catgatcaac gccgtgcgat gctccgggtt
     7501 taatggttca tcgccaactg ttgacgcaga tatactgtat ttatcgcgac caataagcag
     7561 ttgcaggaag cccattaacg ccgtccagaa tccggcggac acccaacaga acagtaccgc
     7621 aaagaggatc aggataccgg tttgcagcat ataaggcaga agctgcataa aggaaaccca
     7681 caaatcctga ccaaccatat ccataggatt aatcagcgcc caaccctgat aaggaagaat
     7741 ggtcttcata taccaggtcg cgacgacagt ttgcgcgagc gtcaggatca acagaatgta
     7801 acggcggatg gtaccgacgg tacgccactt ttgctcgctc tcctgctctt ctttggtcaa
     7861 acgagccaga tagcgcggcg tgacatcgcg tccacgcagg cgatcccaga aacggcctac
     7921 cgggttggta cgccacgggt cgggaaacat cgaggagcgt tttgcttctg gcatcgcctt
     7981 cagctgatcg cgcccttcgt cgtctttaat taactgtcca tcagcaagtg aatctggcca
     8041 ggcttgttcc agacgcgcct ttaccgagcc ttgcggggaa tcatcctccc gcgcccaggt
     8101 gcggtgttcg gcatccagcg cctgatgaac ggcgcggata tcagtcttcg gcaatgccgc
     8161 tttctcgctt gcggcgatgg gcattgcgtc aatgtactca gttgtcttat tcattggcag
     8221 gtaactggta gctccaggtt tcactcaacg tctgatcggc attcaccagc gcagcacgca
     8281 tttcagtggt tttcttggca tctttcactt tcacacgcat caccagacgc cagcctttgg
     8341 taaccgggtt atagcgcacc gtgctttcaa ctatctcacc attatcacca atgctggttt
     8401 gcgctgtgac cggggtatcc tctggcagtt ttttcatctc tgcgccggta aaatcgacca
     8461 caaaggcgat agtaccgtca ggctggcgaa tcaggttcga ctgcttcaca tcccccgttg
     8521 aacgacgcgt ttgttgcacc catgcgttat ctggcgcatg cagtttgtct tcatcacggc
     8581 tgaaggtgat ggtgtattta aagttcatct ctttacccgg ctccggcagc tgatccggcg
     8641 tccagtaagc gacgatgtta tcgttggttt catcgttggt tggaatttcc accagctcaa
     8701 cgctgccttt accccactcc cctttcggag tcacccatgc gcttggacga agatcgtaac
     8761 gatcatcgag atcttcaaag cgggagaaat cacgaccgcg ctgcaacaga ccgaagcctt
     8821 gcgggttctc catcgagaag ctgctgaccg ctaaatgttt cgggttattc aacggacgcc
     8881 agatccattc gccattaccg gcatggatag acagaccgtt agagtcgtgc aactccggac
     8941 gatagttatt tgcaggcgac ggttggttcg gcccaaacag gaacatactg gttaacggtg
     9001 caacccccag tttgccgact ttatcgcgca gatagatttt cgactgcaca tccacaaccg
     9061 tgtcacgccc tggcataacc acgaatttgt aagcacctgt cgcgcgcggc gagtcaagca
     9121 atgcataaat ggttaaacgt ttatcagtcg gttttggacg ctcgatccag aactctttga
     9181 agcgtggaaa ttcttcaccc gatggcaagg cggtatcaat tgccaggccg cgggcagaaa
     9241 ggccataaac ctgacctgca ccaatcacgc ggaaatagct ggccccgagc atgctgacga
     9301 tttcatcgtt tttatctttg ctgttgatcg ggtaaagcac tttaaaaccg gcaaaaccaa
     9361 ggtcttttac cgtgtctttg tcatgctgaa catcgccgaa agtgaaataa tccgggctgt
     9421 atttgattcg tttgactgcg gtggcagtca cttcatttat tttgaccggg gtatcgaagt
     9481 acataccctg atggtagaac tcgagtttga atggggtctt cagattgttc cagtacgctt
     9541 tgtcatgatt aaactggatc tgctgatagt ccgcgtattt catatcgcgg aaaacggagg
     9601 gcaagttgct tttgggcgcc tcatagcctt tcccggctaa ggattgagct tgctttgcga
     9661 catcatcaat actgaaagcc cagcttgaag atgtatacag ggttaacatt actgcagcac
     9721 tcaaccaacg cattttcatc atttgtagtt tatgtttcat aattagtaag cacttccccc
     9781 tttgtgtgct tatatcgatc cgatctattt taatggataa ccagccattc cgacaacaga
     9841 atcccggtat cgttcagcac gctggctcat ggattaagca cctgattgca accgttttca
     9901 ctttccgtgc ttctcctaaa gacagaagca tcgacttcgt gtagggtttg ccagcaattt
     9961 tatttatcaa ccgtcttaca gacgcttcca ttctggttag tggttgcgat gatgctatgc
    10021 ataaaaacct tgaagcctgc gcctggcgcg gtcaggtcgg tgtcgctatg gcaacaagcc
    10081 taaaggcata acccgacagg gataagacga aaaatccgag attagttaac catatatgaa
    10141 tccagtaccc gcgcaacgtg aatatttcct tgactccatc cgcgcctggc tgatgttgtt
    10201 agggatccct tttcatattt ctttaatcta ttcgagccat acatggcatg tgaatagcgc
    10261 cgaaccgtca ttgtggctga ccctttttaa tgacttcatc cactcgttcc gcatgcaggt
    10321 atttttcgtt atatccggct acttttccta catgcttttt ttacgctatc ccttgaaaaa
    10381 atggtggaaa gtacgtgtcg aacgtgtagg tatcccgatg ttaacagcca tccccttact
    10441 gacattgccg caatttatta tgctgcaata cgtcaaaggg aaagcggaaa gttggcctgg
    10501 gctgtcattg tatgacaaat ataatacgtt ggcctgggaa ttaatatcac acctgtggtt
    10561 tttactggtg ttagtggtca tgacgacgct gtgcgta
//
LOCUS       DAPHBJ010000155        10595 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.157, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000155 DAPHBJ010000000
VERSION     DAPHBJ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.157"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            141..476
                     /gene="eutS"
                     /locus_tag="RHZ76_003453"
     CDS             141..476
                     /gene="eutS"
                     /locus_tag="RHZ76_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HDV2814041.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            489..968
                     /gene="eutP"
                     /locus_tag="RHZ76_003454"
     CDS             489..968
                     /gene="eutP"
                     /locus_tag="RHZ76_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HDV2814042.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKSDIDT
                     PGEYFNHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            943..1644
                     /gene="eutQ"
                     /locus_tag="RHZ76_003455"
     CDS             943..1644
                     /gene="eutQ"
                     /locus_tag="RHZ76_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HDV2814043.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGQTMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            1641..2444
                     /gene="eutT"
                     /locus_tag="RHZ76_003456"
     CDS             1641..2444
                     /gene="eutT"
                     /locus_tag="RHZ76_003456"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HDV2814044.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSMVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            2441..3457
                     /gene="pta"
                     /locus_tag="RHZ76_003457"
     CDS             2441..3457
                     /gene="pta"
                     /locus_tag="RHZ76_003457"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDV2814045.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            3496..3789
                     /gene="eutM"
                     /locus_tag="RHZ76_003458"
     CDS             3496..3789
                     /gene="eutM"
                     /locus_tag="RHZ76_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HDV2814046.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            3896..4183
                     /gene="eutN"
                     /locus_tag="RHZ76_003459"
     CDS             3896..4183
                     /gene="eutN"
                     /locus_tag="RHZ76_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HDV2814047.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            4195..5598
                     /locus_tag="RHZ76_003460"
     CDS             4195..5598
                     /locus_tag="RHZ76_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HDV2814048.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            5609..6445
                     /gene="eutJ"
                     /locus_tag="RHZ76_003461"
     CDS             5609..6445
                     /gene="eutJ"
                     /locus_tag="RHZ76_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HDV2814049.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGLRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCLQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            6435..7622
                     /gene="eutG"
                     /locus_tag="RHZ76_003462"
     CDS             6435..7622
                     /gene="eutG"
                     /locus_tag="RHZ76_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HDV2814050.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            7839..9065
                     /gene="eutH"
                     /locus_tag="RHZ76_003463"
     CDS             7839..9065
                     /gene="eutH"
                     /locus_tag="RHZ76_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDV2814051.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            9062..10465
                     /gene="eutA"
                     /locus_tag="RHZ76_003464"
     CDS             9062..10465
                     /gene="eutA"
                     /locus_tag="RHZ76_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDV2814052.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
BASE COUNT         2274 a         2802 c         3211 g         2308 t
ORIGIN      
        1 atgaaagttt atgcaccaca gcgaatatct ctccattctt agtgatctac ctcacctttt
       61 aaacgcgctt gccgaatttt gttatttact ctgacgaaaa attgtcacga tacacgaaag
      121 tttttcacag gcggcgactc atggataaag aacgcatcat tcaggaattt gtgcccggca
      181 aacaggtcac actggcgcat ctcatcgcgc atcccggtga ggaactggcg aagaaaatcg
      241 gcgttccgga tgcgggcgca attggcatta tgacgctaac tcccggcgaa acggcgatga
      301 tcgccggtga tttagcgctg aaagccgctg atgtgcatat cggttttctc gaccgattca
      361 gcggtgcatt ggtgatttat ggctcggtgg gcgcagtaga agaagcgtta tcacaaaccg
      421 tcagcggttt aggtcgctta ttaaattata cgctttgtga aatgaccaaa agttaatacg
      481 aggcattcat gaaacgaatt gcgtttgtgg gtagcgtcgg ggcaggaaaa acaacgttat
      541 ttaatgcgtt gcaggggaat tataccctcg ccagaaaaac acaggccgtg gaatttaatg
      601 ataagagcga tattgatacg ccgggtgaat attttaacca tccccgctgg tatcacgcct
      661 taattaccac gctacaggat gtagacatgc tgatctatgt ccatggcgca aatgacccgg
      721 aaagtcggtt gcctgccggg ttgctggata ttggcgtcag caaacggcaa atcgccgtca
      781 tcagtaaaac ggacatgcca gatgctgacg tcgccgcaac gcgaaagtta ttgcttgaga
      841 cgggtttcga agagccgatt ttcgagctaa acagccacga cccgcaaagc gtgcagcaac
      901 tggtggatta tctggcatct ctcaccaaac aggaggaagc aggtgaaaaa actcatcaca
      961 gcgaatgata ttcgtgaagc acacgcgcgc ggcgaacagg caatgtccgt ggttctgcgc
     1021 gccagcatta ttaccccgga agcccgtgaa gtggcggatc tactgggttt taccatcacc
     1081 gaatgtgatg aatcgatccc ggtaacggcg tccgtgcccg ccagcgtgcc agccgacaaa
     1141 accgaaagcc agcgcatccg cgaaaccatc attgcccagc tgccggaagg ccagtttacc
     1201 gaaagcctgg tcgcacagct gatggaaaaa gtgatgaagg aaaaacagtc gctggagcag
     1261 ggcgcaatgc agccgagctt caaatcggtg accggcaaag gcggcatcaa agtgattgat
     1321 ggcagtagcg tcaagtttgg tcgtttcgac ggcgcagagc cgcattgcgt gggcttgact
     1381 gatcttgtga ccggagacga cggcagcagc atggccgccg gttttatgca gtgggagaac
     1441 gcattcttcc cgtggaccct gaactacgac gaaatcgaca tggtgctgga aggggagctg
     1501 catgttcgcc acgaaggcca aaccatgatc gccaaagcag gcgacgtgat gtttatcccg
     1561 aaaggctcca gcatcgaatt tggtacgaca tccagcgtga aattcctgta tgtcgcctgg
     1621 ccggctaact ggcaatccct atgaaagatt tcatcaccga agcatggcta agagcgaacc
     1681 atacgctcag cgaaggcgca gagatccatc tccctgcgga cagtcgcctg acgccctctg
     1741 cccgggagtt gctggaaagc cgccatctgc gcatcaagtt tattgacgag cagggccgcc
     1801 tgtttgttga cgatgaacag cagcagccgc agcccgttca tgggctgacc agtagcgatg
     1861 aacatccgca ggcgtgctgc gaactgtgtc gccagccggt ggcaaaaaag ccggatacgc
     1921 tgactcacct gtcggcggaa aaaatggtcg ccaaaagcga tccgcgtctg ggttttcgcg
     1981 cggttctcga cagcaccatt gcgctggcag tgtggctgca aattgaactg gcggaaccgt
     2041 ggcagccgtg gctggcggat atccgttcgc gtcttggcaa cattatgcgc gccgatgcgc
     2101 tgggggaacc gctgggctgc caggcgattg tcgggctttc tgacgaagat ctgcatcggc
     2161 tttctcatca gccgctgcgc tatctcgacc acgatcatct ggtgccggaa gccagccacg
     2221 ggcgcgatgc tgcgttgctc aatctgctac gcaccaaagt gcgggaaacc gaaaccgtag
     2281 cggcgcaggt ctttatcacc cgcagctttg aagtattacg cccggacatt ttgcaggcgc
     2341 tgaaccgcct ttcgagcatg gtctacgtga tgatgatcct gagcgtaacg aagcagccgc
     2401 taacggtgaa gcagatccaa caacgactgg gggaaacgca atgattattg aacgttgtcg
     2461 tgaactggcg ttgcgagcgc ccgccagagt ggtttttccg gatgcgttag accaacgtgt
     2521 gctgaaagcc gcgcaatatt tacatcaaca aggtctggca acgcccattc tggtcgccaa
     2581 tccgtttgaa cttcgtcagt ttgcgctcag tcacggcgtg gcgatggacg ggctacaggt
     2641 gattgatccg catggcaacc tcgcaatgcg ggaagaattt gctcatcgct ggctggcccg
     2701 cgcgggcgaa aaaacgccgc cggatgcgct ggaaaaactt accgaccctc tgatgttcgc
     2761 cgcagcaatg gtcagcgccg gtaaagcgga tgtctgtatc gcgggcaacc tctcttccac
     2821 ggcaaatgtg ctgcgtgccg gattacgcat tatcggcttg cagccaggct gtaaaacgct
     2881 ctcttccatt ttcctgatgt tgccacagta cagcggcccg gcgttgggct ttgccgattg
     2941 cagcgtggtg ccacagccga cggcggcgca gctggcggat atcgcgcttg ccagtgccga
     3001 aacctggcgc gccatcaccg gagaagagcc gcgcgtggcg atgctgtcgt tttccagcaa
     3061 tggtagcgcc cgtcacccct gtgttgctaa cgtccagcag gcgacagaaa tcgtccgtga
     3121 gcgcgcacca aagctggtgg tggatggcga gttacagttt gacgccgcct tcgtgccgga
     3181 agtggcggcg caaaaagcgc ctgccagccc gctacagggc aaggccaatg tgatggtttt
     3241 tccgtcgctg gaagccggaa atattggtta caaaatcgca caacgactcg gcggatatcg
     3301 tgccgtcggg ccactgatac aaggacttgc cgcgccgatg cacgatctct ctcgtggttg
     3361 tagcgtgcag gaaattatcg agctggcgct ggtggcagct gtgccgcgtc agacagaagt
     3421 gaaccgcgaa agcagtttac aaacactggt tgaatgaacg gtcccgttct ggaccctgat
     3481 aaagaggaaa gcacaatgga agcattagga atgatcgaaa cccggggcct ggttgcactg
     3541 attgaggctt ctgacgcgat ggtgaaagct gcgcgcgtca agctggttgg tgttaagcag
     3601 attggcggcg gcctgtgtac tgccatggtt cgtggtgatg ttgcagcctg taaagccgcg
     3661 accgatgcag gtgccgcagc ggcacagcgt atcggcgagc tggtatctgt acacgttatc
     3721 ccgcgtccgc acggcgacct ggaagaagtc ttcccgatcg gcctgaaagg cgatagcagc
     3781 aacctgtaat caccctgtgt gacgtcgatt cgggcgtcac gctctctcct ttttcatttt
     3841 accttctgcg gagggtaggg ggagctttta cctgaaatac gccacggagg cgggtatgaa
     3901 actggcagtc gtcactggac aaattgtttg taccgtacgc catcacggac tggcgcatga
     3961 caaattgctg atggtggaaa tgattgatcc acaaggtaat cctgacgggc aatgcgccgt
     4021 cgccatcgac aatattggcg cgggaaccgg ggagtgggtg ttactggtga gtggcagttc
     4081 cgcccgccag gcgcataaaa gcgaaacgtc accggtcgat ctgtgcgtga ttggcattgt
     4141 cgatgaggtg gtgtctggcg gtcaggtaat tttccataaa taaggcagaa catcatgaat
     4201 caacaggata ttgaacaggt ggtgaaagcg gtactgctga aaatgcaaag cagtgacacg
     4261 ccgcccgccg ccgttcatga gatgggcgtt ttcgcgtccc tggatgacgc cgttgcggca
     4321 gccaaagtcg cccagcaagg gttaaaaagc gtggcaatgc gccagttagc cattgctgcc
     4381 attcgtgagg caggcgaaaa acacgccaga gatttagcgg aacttgccgt cagtgaaacc
     4441 ggcatgggcc gcgttgaaga taaatttgcc aaaaacgtcg ctcaggcgcg cggcacgcca
     4501 ggcgttgagt gtctctctcc gcaggtgctg accggcgaca acggcctgac tttaattgaa
     4561 aacgcgccct ggggcgtggt ggcttcggta actccgtcca ctaacccggc ggcaaccgta
     4621 attaataacg ccatcagcct gattgccgcg ggcaacagcg tcatttttgc cccgcatccg
     4681 gcggcgaaaa aagtctccca gcgggcgatc acgctgctca accaggcgat tgttgccgca
     4741 ggcgggccgg aaaacttact ggttactgtg gcaaatccgg atatcgaaac cgcgcagcgt
     4801 ttgttcaagt ttccgggcat cggccttctg gtggtgaccg gcggcgaagc ggtagtagaa
     4861 gcggcgcgta aacacaccaa taaacgtctg attgccgcag gcgctggcaa cccgccggta
     4921 gtggtggatg aaaccgccga cctcgcccgt gccgctcagt ccatcgtcaa aggcgcttct
     4981 ttcgataaca acatcatttg tgccgacgaa aaggtactga ttgttgttga tagcgtagcc
     5041 gatgaactga tgcgtctgat ggaaggccag cacgcggtga aactgaccgc agaacaggcg
     5101 cagcagctgc aaccggtgtt gctgaaaaat atcgacgagc gcggaaaagg caccgtcagc
     5161 cgtgactggg ttggtcgcga tgcggcgaaa atcgcggcgg caatcggcct gaacgtgccg
     5221 caagaaacgc gtttgctgtt tgtggaaacc actgcagaac atccgtttgc cgtgaccgaa
     5281 ctgatgatgc cggtgctgcc cgtcgtgcgc gtcgccaacg tggcggatgc cattgcgctg
     5341 gcggtgaaac tggaaggcgg ttgccaccac acggcggcaa tgcactcgcg caacatcgaa
     5401 aacatgaacc agatggcgaa tgctattgat accagcattt tcgttaagaa cggaccgtgc
     5461 attgccgggc tggggctggg cggggaaggc tggaccacca tgaccatcac cacgccaacc
     5521 ggtgaagggg taaccagcgc gcgtacgttt gtccgtctgc gtcgctgtgt attagtcgat
     5581 gcgtttcgca ttgtttaagg agtaacagat ggcgcacgac gaacaatggc tcaccccacg
     5641 tctgcaaacg gcggcaacgc tgtgtaacca gacgcccgcc gcgacggaat ctccgctgtg
     5701 gctgggggtg gatctgggca cctgcgatgt ggtgtcgatg gttgttgacc gcgacggtca
     5761 gccggtggcg gtatgcctcg actgggccga tgtggtccgc gacggcatcg tctgggattt
     5821 cttcggtgcg gtcaccattg ttcgtcgcca tctcgacact cttgagcagc aattcggtct
     5881 tcgttttagc catgcggcga cttcatttcc tccaggtacg gacccgcgta tttccatcaa
     5941 cgtgctggaa tctgccgggc tggaagtgag ccatgtgctg gatgagccaa ccgcagttgc
     6001 ggatctgctg caactggaca acgccggtgt agtggatatc ggcggcggca ctaccggcat
     6061 tgccatcgtg aaaaagggca aggtgacgta ctcggcggat gaagccaccg gcggacatca
     6121 catctctctg actctcgccg gaaatcgccg tatttcgctg gaagaggcgg agcagtacaa
     6181 gcgcggtcac ggtgaagaga tttggcctgc ggtgaaaccg gtgtacgaaa aaatggcgga
     6241 cattgtcgcc cggcatattg aagggcaggg cattactgat ttatggctgg cgggcggctc
     6301 ttgtttgcaa ccgggcgtgg cggaactgtt tcgcaaacaa ttcccggcgt tacaggtgca
     6361 tttaccgcag cacagtttgt ttatgacccc gctggcgatc gccagtagtg ggcgagagaa
     6421 agcggagggg ctatatgcaa aatgaattgc agaccgcgct ctttcaggcg ttcgataccc
     6481 tgaatctgca acgggtaaaa acatttagcg ttccaccggt gacgctttgc ggtccgggcg
     6541 cggtgagcag ttgcgggcag caagcgcaaa cgcgtgggct gaaacatctg ttcgtgatgg
     6601 cagacagctt tttgcatcag gcggggatga ccgccgggct gacgcgcagc ctggctgtta
     6661 aaggcatcgc catgacgctc tggccatgtc cggtgggcga accgtgcatt accgacgtgt
     6721 gtgcagccgt ggcgcagttg cgtgagtcag gctgtgatgg ggtgatcgca tttggcggcg
     6781 gctcggtgct ggatgcggcg aaagccgtgg cgttgctggt gacgaacccc gatagcacgc
     6841 tggcagagat gtcagaaacc agcgttctgc aaccgcgctt gccgctgatt gccattccaa
     6901 cgaccgccgg aaccggctct gaaaccacca atgtaacggt gattatcgac gcggtgagcg
     6961 ggcgcaagca ggtgttagcc catgcctcgc tgatgccgga tgtggcgatc ctcgacgccg
     7021 cattgaccga aggtgtgccg tcgcatgtca cggcgatgac cggcattgat gcgttaaccc
     7081 atgccattga agcatacagc gccctgaacg ctacaccgtt taccgacagc ctggcgattg
     7141 gtgccattgc gatgattggc aaatcgctgc cgaaagcggt gggctacggt cacgaccttg
     7201 ccgcgcgcga gagcatgtta ctggcttcat gtatggcggg aatggcgttt tccagtgcgg
     7261 gtcttgggtt gtgccacgcg atggcgcatc agccgggcgc ggcgctgcat attccgcacg
     7321 gtctcgcgaa cgccatgttg ctgccaacgg tgatggaatt taaccggatg gtttgtcgtg
     7381 aacgctttag tcagattggt cgggcactgc gaactaaaaa atccgacgat cgtgacgcta
     7441 ttaacgcggt aagtgagctg attgcggaag ttgggattgg taaacgactg ggcgatgttg
     7501 gtgcgacatc tgcgcattac ggcgcatggg cgcaggccgc gctggaagat atttgtctgc
     7561 gcagtaaccc gcgtaccgcc agcctggagc agattgtcgg cctgtacgca gcggcgcaat
     7621 aaatgccgga tgcgacgctt gccgcatctt atccagccta cgggattgca catgtagggc
     7681 ggataaggcg ttcacgccgc atccgccagt ggcgcggtgc aattgccgga tgcgacgctt
     7741 gccgcgtctt atccagccta cgggattgca catgtagggc ggataaggcg tttacgccgc
     7801 atccgccaat aaataacaac taacagggag taaaggcgat gggaattaac gaaatcatca
     7861 tgtacatcat gatgttcttt atgctgatag ctgccgtaga caggatcctg tcgcagttcg
     7921 gcggttctgc tcgtttcctc ggtaagttcg gtaaaagtat cgaaggatca ggcggtcagt
     7981 tcgaagaagg ctttatggca atgggcgcac tgggcctggc gatggtcggt atgaccgcgc
     8041 tggcaccggt actggctcac gttctcgggc cggtaattat tccggtttac gaaatgctcg
     8101 gcgctaaccc atcgatgttc gccggaacac tgctggcgtg cgatatgggc ggcttcttcc
     8161 tcgccaaaga gctggcgggc ggcgacgtag ccgcgtggct atactctggg ttaattctcg
     8221 ggtcgatgat ggggccaacg attgtgtttt ccattccggt ggcgctcggc attatcgaac
     8281 cttctgaccg tcgttatctg gcgctcggcg tgctggcggg cattgtgacc attccgattg
     8341 gttgtatcgc tggtggtctg gttgctatgt actccggtgt gcagatcaac ggccagccgg
     8401 tggaattcac tttcgccctg atcctgatga acatgatccc ggtgatcatt gttgcgattc
     8461 tggtggcgct ggggctgaaa ttcatcccgg aaaaaatgat caacggcttc cagatcttcg
     8521 ccaaattcct cgttgcattg atcaccctcg gtcttgccgc tgcggtagtg aaattcctgc
     8581 ttggctggga actgatcccc ggtctggatc ctatctttat ggcccctggc gataaacccg
     8641 gtgaggtgat gcgcgccatt gaagttatcg gttctatctc ctgcgttctg ttaggggcgt
     8701 atccgatggt gctgctgctg actcgctggt ttgaaaaacc gctgatgagc gtcggtaaag
     8761 tactgaatat gaacaacatc gcggcagccg gcatggtggc aacgcttgcc aacaacatcc
     8821 cgatgttcgg catgatgaag cagatggata cccgcggcaa agtcatcaac tgcgccttcg
     8881 ccgtttccgc tgctttcgcc ctgggcgacc acttaggctt cgccgctgcc aacatgaacg
     8941 ccatgatctt cccgatgatt gtcggcaagt tgatcggcgg cgtaacggcg attggcgtgg
     9001 cgatgatgct ggtgccaaaa gaagacgcga ccgcgactaa aaccgaagcg gaggcacaat
     9061 cgtgaacact cgccagctat tgagcgtcgg tatcgatatc ggcaccacca ccacccaggt
     9121 gattttctcc cacctggagc tggttaaccg tgcggcggtg tcgcaggtgc cgcgctacga
     9181 attcatcaaa cgcgaaatta gctggcaaag cccggtgttc tttacccctg tcgataaaca
     9241 gggtggttta aaagaagcgg aactgaaatc gctgatttta gagcaatatc aggctgcggg
     9301 cattgcgccg gaaagcgttg attctggtgc catcatcatc accggtgaaa gcgcgaaaac
     9361 ccgcaatgct cgcccggcgg tgatggcgct ctctcagtcg ctgggcgatt ttgtcgtcgc
     9421 cagcgccggg ccgcacctcg aatccgtgat cgccggtcac ggagccgggg cgcaaaccct
     9481 ttctgaacaa cggctgtgtc gggtactgaa tatcgacatc ggcggtggca ccgcgaacta
     9541 cgccctgttc gatgccggaa aaatcagcgg cactgcctgt ctcaacgtcg gtggtcgcct
     9601 gctggaaacc gacagccagg ggcgcgtggt ttacgctcat aaaccggggc agatgattgt
     9661 ggatgagtgc ttcggtgcag gcactgacgc ccgttcgctg accggcgcgc agctggtgca
     9721 ggttacccgg cggatggcgg cgctgattgt cgaagtgatt gacggaacgc tttcgccgct
     9781 cgcgcaggca ttgatgcaaa ccggtttgct gcccgcaggt gttacgcccg aaatcattac
     9841 gctttctgga ggcgtgggcg aatgttatcg ccaccagccc gccgacccgt tctgttttgc
     9901 cgatattggc ccacttctgg caacggcgct gcatgaccat ccgcgcctgc gtgagatgaa
     9961 tgtgcagttt ccggcgcaaa ccgtacgcgc cacggtgatt ggcgcgggtg cacataccct
    10021 ttcgctctct ggcagcacaa tctggctgga gggcgtacaa ctgccgctgc gcaatttgcc
    10081 ggtggcgatc ccgattgatg aaacggatct ggtgagtgcc tggcaacagg cgctgcttca
    10141 gctggatctt gatcccaaaa ctgacgcgta cgtgctggcg cttcccgcct cgctgcctgt
    10201 gcgttacgcc gcggtactga cggtcatcaa cgcgctggtc gatttcgtcg cgcgttttcc
    10261 gaatccgcat cccctgctgg tggtggccgg gcaggacttt ggtaaagctc tgggcatgtt
    10321 gttgcgccca cagctacaac aactcccgtt ggcagtcatt gacgaagtga ttgtccgcgc
    10381 gggggactat atcgacattg gtacgcctct ttttggcgga tcggttgtgc cggtgacggt
    10441 gaaatcactc gcatttcctt cctgaatacg gtagttatca ggtatggtgc ttggtgactg
    10501 ggtgttagtg ggattatatc cggcagtgta tccggttaag gtgattggtc tggcaagatg
    10561 attggatgct tttgtggtga aaaatgtttt ggtgt
//
LOCUS       DAPHBJ010000156        10285 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.158, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000156 DAPHBJ010000000
VERSION     DAPHBJ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.158"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            49..624
                     /gene="ssuE"
                     /locus_tag="RHZ76_003465"
     CDS             49..624
                     /gene="ssuE"
                     /locus_tag="RHZ76_003465"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HDV2814053.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALYRRDVQVPDLLSLRGNAHA"
     gene            617..1576
                     /gene="ssuA"
                     /locus_tag="RHZ76_003466"
     CDS             617..1576
                     /gene="ssuA"
                     /locus_tag="RHZ76_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HDV2814054.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1573..2718
                     /gene="ssuD"
                     /locus_tag="RHZ76_003467"
     CDS             1573..2718
                     /gene="ssuD"
                     /locus_tag="RHZ76_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HDV2814055.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLQGETVDFNGKHIHVRGA
                     KLLFPAIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPLLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2729..3520
                     /gene="ssuC"
                     /locus_tag="RHZ76_003468"
     CDS             2729..3520
                     /gene="ssuC"
                     /locus_tag="RHZ76_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HDV2814056.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3517..4284
                     /gene="ssuB"
                     /locus_tag="RHZ76_003469"
     CDS             3517..4284
                     /gene="ssuB"
                     /locus_tag="RHZ76_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HDV2814057.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAIAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(4327..6939)
                     /gene="pepN"
                     /locus_tag="RHZ76_003470"
     CDS             complement(4327..6939)
                     /gene="pepN"
                     /locus_tag="RHZ76_003470"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HDV2814058.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQLLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7205..8407
                     /gene="pncB"
                     /locus_tag="RHZ76_003471"
     CDS             7205..8407
                     /gene="pncB"
                     /locus_tag="RHZ76_003471"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDV2814059.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8576..9976
                     /gene="asnS"
                     /locus_tag="RHZ76_003472"
     CDS             8576..9976
                     /gene="asnS"
                     /locus_tag="RHZ76_003472"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDV2814060.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAAARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2314 a         2635 c         2783 g         2553 t
ORIGIN      
        1 tgttatcaat gttaacaaaa aaagaacaat tggttataag gagagagtat gcgtgtcatc
       61 accctggcgg gtagtcctcg ctttccttct cgctccagct ccttgctgga atatgcgcgg
      121 gaaaaactaa atggcctgga tgtagaggtt tatcactgga atctgcaaaa cttcgccccg
      181 gaagatctgc tttatgctcg tttcgatagt ccggcactca agaccttcac cgaacagctg
      241 caacaggctg atggactgat agtcgctaca cctgtgtata aagccgccta ttccggcgcg
      301 ctgaaaaccc tgctcgacct gctgccagag cgcgctttgc aaggcaaagt ggtgctaccg
      361 ctggcgacgg gcggtaccgt ggcccatctg ctggcggtcg attatgccct taaaccagtt
      421 ttaagcgcac tgaaagctca ggagatcctg cacggcgtgt ttgccgatga ctcacaagta
      481 attgattacc atcacagacc ccagttcacg ccaaatctgc aaacccgtct tgataccgcg
      541 ctagaaactt tctggcaggc attgtaccgc cgcgatgttc aggttcctga ccttctgtct
      601 ctgcgaggta atgcccatgc gtaacatcat taaactggcg ctggtgggat tgcttagcgt
      661 ctctacgatt gcggttgctg cagaatcctc gcctgaagcg ttacgtatag gctatcagaa
      721 aggcagtatt ggtatggtac tggcaaaaag ccaccagtta ctggaaaaac gctatccgca
      781 atcaaaaatc tcctgggtgg agttccccgc gggtccgcaa atgttggaag cgttaaacgt
      841 tggcagtatt gatctcggca gtaccgggga tattccgcca atctttgccc aggctgccgg
      901 ggctgatttg gtatatgtgg gcgtcgagcc accgaagccc aaagccgaag tgattctggt
      961 ggcagaaaac agcccgatca aaaccgtagc cgatcttaaa ggtcacaaag ttgcctttca
     1021 gaaaggttcc agttcacaca accttttact gcgtgcactg cgtcaggccg gacttaagtt
     1081 taccgatatc caacccactt acctgacgcc cgctgatgcc cgcgccgcgt tccagcaagg
     1141 taacgttgac gcctgggcta tctgggatcc ctactactct gctgcattat tacagggcgg
     1201 cgtgcgggtg ctgaaagacg gcaccgatct caatcaaact ggatcgtttt atctggcagc
     1261 tcgtccctat gcagaaaaaa acggcgcttt tattcagggc gtactggcaa cctttagtga
     1321 ggccgatgcg ttaacccgca gccagcgcga gcaaagcatc gctttactgg caaaaacgat
     1381 gggcttaccg gcaccggtga ttgcctctta cttagatcat cgccctccta ccaccatcaa
     1441 accggttaac gccgaggttg ccgccttaca gcagcaaacg gcagatctgt tttatgaaaa
     1501 tcgtctggtg ccgaaaaaag tcgatattcg ccagcgcatc tggcagccca ctcaactgga
     1561 aggaaaacaa ttatgagtct gaatatgttc tggtttttac cgacccacgg tgacgggcat
     1621 tatctgggaa cggaagaagg ttcacgcccg gttgatcacg gttatctgca acaaattgcg
     1681 caagcggcgg atcgtcttgg ctataccggt gtgctaattc caacggggcg ctcctgcgaa
     1741 gatgcgtggc tggttgccgc atcgatgatc ccggtgacgc agcggctgaa gtttcttgtc
     1801 gccctgcgtc ccagcgtaac ctcacctacc gttgccgccc gccaggccgc cacgcttgac
     1861 cgtctctcaa atggacgtgc gttgtttaac ctggtcacag gcagcgatcc acaagagctg
     1921 gcaggcgacg gagtgttcct tgatcatagc gagcgctacg aagcctcggc ggaatttacc
     1981 caggtctggc ggcgtttatt gcagggagaa accgtcgatt tcaacggtaa acatattcat
     2041 gtgcgcggag caaaactgct cttcccggcg attcaacagc cgtatccgcc actttacttt
     2101 ggcggatcgt cagatgtcgc ccaggagctg gcggcagaac aggttgatct ctacctcacc
     2161 tggggcgaac cgccggaact ggttaaagag aaaatcgaac aagtgcgggc gaaagctgcc
     2221 gcgcatggac gcaaaattcg tttcggtatt cgtctgcatg tgattgttcg tgaaactaac
     2281 gacgaagcgt ggcaggccgc cgagcggtta atctcgcatc ttgatgatga aactatcgcc
     2341 aaagcacagg ccgcattcgc ccggacggat tccgtagggc aacagcgaat ggcggcgtta
     2401 cataacggca agcgcgacaa tctggagatc agccccaatt tatgggcggg cgttggctta
     2461 gtgcgcggcg gtgccgggac ggcgctggtg ggcgatggtc ctacggtcgc tgcgcgaatc
     2521 aacgaatatg ccgcgcttgg catcgacagt tttgtgcttt cgggctatcc gcatctggaa
     2581 gaagcgtatc gggttggcga gttgctgttc ccgcttctgg atgtcgccat cccggaaatt
     2641 ccccagccgc agccgctgaa tccgcaaggc gaagcggtgg cgaatgattt tatcccccgt
     2701 aaagtcgcgc aaagctaagg aagaaataat ggcaacgcca gtgaagaagt ggttattgcg
     2761 cgttgccccc tggtttttac cggtgggcat cgtggcggtg tggcaactgg cctcctcggt
     2821 tggctggctg tcgacgcgta ttttgccttc accggaagga gtggtgacgg cgttctggac
     2881 gctctccgcc agcggcgaac tgtggcagca tctggcgatc agctcctggc gggcgctgat
     2941 tggcttttca attggcggat cgctggggtt gattttggga ctgattagcg ggctatctcg
     3001 ctggggagaa cggctgctgg atacctcaat tcagatgttg cgcaacgtgc cgcatctggc
     3061 gctgatcccg ctggtgattt tgtggtttgg cattgatgaa tccgcaaaaa tctttctggt
     3121 ggcgctcggt acgctattcc ccatttatat caacacctgg catggcatcc gtaatatcga
     3181 tcgcgggttg gtggagatgg cgcgtagcta tggattatcc ggcataccgc tgtttatcca
     3241 tgtgatcctg cctggtgccc tgccctcaat tatggtcgga gtgcgttttg cgttgggcct
     3301 gatgtggctg acgctgattg ttgccgaaac catttctgcc aattccggca ttggttatct
     3361 ggcgatgaat gcgcgggagt ttttgcaaac ggacgtggtg gtagtcgcta ttattcttta
     3421 cgccctgctc ggcaaactgg ctgacgtcag cgcgcagctg ttagagcgcc tctggttgcg
     3481 ctggaacccg gcttatcatt tgaaggaggc cacggtatga atactgctcg tctgaaccag
     3541 ggcacaccat tgttgctcaa tgcagtaagc aaacattacg cggaaaatat cgtcctgaac
     3601 caactggatt tacatattcc ggcaggtcag tttgtggcgg tggtgggccg cagcggtggt
     3661 ggcaaaagta ccctgctgcg cctgctggca ggtctggaaa cgccaaccgc aggcgatgtg
     3721 ttagcgggca ccacaccgct ggctgaaatt caggaagaca cgcgaatgat gtttcaggat
     3781 gcgcgtctgc taccgtggaa atcggtgatt gataacgttg ggttaggcct taaaggccag
     3841 tggcgcgatg ccgcacgtcg agcgttggct gcggtaggac tggagaatcg cgccggggaa
     3901 tggcctgccg cactttctgg cgggcagaaa cagcgagtgg cgctggcaag agcgttgatt
     3961 catcgaccgg gtttattgtt gcttgatgaa ccgctcggtg cgctggatgc cttaacgcga
     4021 ctcgagatgc aggatttgat tgtgtcactt tggcaggagc acggctttac cgtactgttg
     4081 gtgacgcatg atgtgagcga agcgatagca atggctgacc gggtgttgtt aattgaagag
     4141 ggaaaaattg gtctggattt gacggtggat atcccccgtc cgcgccggtt agggtcggtg
     4201 cgattagcgg agctggaagc ggaagtgttg caacgggtaa tgcagcgtgg tgagtctgaa
     4261 actcgcctga gaaaacaggg gtaaaatccc cgaatggcgg cgctattgcc gccattcggt
     4321 tatttattaa gccagtgctt tggtgatctt ctcgtacaga tcgccagaga gattttccag
     4381 ccctttcaac tgttccagcg ccgcgcgcat tttctcctga cgtttggcat cataacgttt
     4441 caggcgaatc agcggctcaa tcaggcgcga agccacctgc gggttacggc tgttgaggtc
     4501 ggtaagcatt tccaccagga gctggtaacc gctgccatct tcggcatgga acgctgccgg
     4561 gttgctgccc gcaaacgcac caatcaacga acgaatacgg ttcgggttgc tcatggtaaa
     4621 tgagcgatgc tgcaacaggc cgcgcaccgt ctccagcaca ttcgccgccg ggctggtggc
     4681 ttgcaggata aaccatttat ccatcaccag accgtcctga tgccacttgt cgtcgtactc
     4741 ctgcatcagc gcgtcacggc atggcagctg tgcagcaacc gccgcagaaa gcgctgccag
     4801 cgcatcggtc atattgtttg cttcatggta ttgcttgctc accagcacgt cagccagatg
     4861 cgtttcacca aaagcaagga agcgcagaca ggcattacgc agagtgcgtt ttgcaatatc
     4921 ttcatgctca acgcggtact cgctctggta attcgcgttg taaatagcca gcagctcatc
     4981 cgccagttca gtcgccagag tacgagtgag tgcttcgcgt acttcggcaa tagcaatcgg
     5041 gtcgatgata tcgaacagtt cagccatttc attgacggat ggcagagtca gaatttctgc
     5101 cgccagtgcc gggtcaatct tctcatcgag cagcaccgcg cggaaggcgt cggccacatg
     5161 caccggcaga gacagcggct gcccttgctg atgacgggcg acgttcagct tgatgtaggt
     5221 tgccagcaaa ctttgcgccg catcccaacg ggagaaatca ttacgcgcat gacgcatcag
     5281 gaaggtcagt tgctgatcgc tccacttata ttccagtttc actggcgcag agaattcgca
     5341 cagcagcgca ggcaccggct ggaagtagac attatcaaag acaaaggtct gttccgcctg
     5401 agtgacgttc agcacggaat tcaccggatg accgcctttc tgcaacggga tcactttgcc
     5461 ttcgttatca tacagttcga tggcaaacgg aatatgcagc ggctgttttt ctgcctgatc
     5521 cggcgtggct ggcgtgcgct ggctgatggt cagggtgtac tgctcggttt ccggattgta
     5581 gtcgtctttg acggtcacaa tcggtgtacc ggactggctg taccaacggc ggaaatggga
     5641 gagatcgaca ttcgacgcat cttccatcgc ctgcacaaag tcgtcacagg tcgctgcact
     5701 accatcatga cgctcgaaat aaagctgcat ccctttctgg aagttttctt cgccaagcag
     5761 ggtgtggatc atgcgaatca cttccgcgcc cttctcgtaa acggtcaggg tgtagaagtt
     5821 gttcatctca atgaccatat ccgggcggat cgggtgcgcc atcgggctgg cgtcttctgc
     5881 aaactgcaat ccgcgcatgg tgcgtacatt attgatgcgg ttaactgcgc gggaaccaag
     5941 gtcagagctg aactcctgat cgcggaagac ggttaaacct tctttcaggc tgagctggaa
     6001 ccagtcgcga caggtcactc ggttaccggt ccagttatgg aaatattcat ggccgataac
     6061 gcgttcaata tcgaggtaat ctttgtcggt ggcggtgtcg gtgcgggcca gcacatattt
     6121 ggagttaaag atattcagcc ccttattctc cattgcgccc atattgaaga aatccaccgc
     6181 gacgatcata tagatgtcga ggtcatactc caggccaaag cgttcttcat cccatttcat
     6241 ggagtttttc agcgaggtca tcgcccacgg cgcgcgatca aggttgccgc gatcgacgta
     6301 cagctccagt gctacttcgc gaccagaacg cgtggtaaag gtatcgcgca gtacatcaaa
     6361 gtcgcctgcc accagcgcaa acaggtagca cggtttcggg aacgggtcct gccactgtac
     6421 ccaatggcgt ccgttttcca gttcgccttg cgcaacgcgg ttaccattgg aaagcaggaa
     6481 gggatatttg attttatcgg caataatttt ggtggtaaaa cgcgccagca cgtccgggcg
     6541 gtcgagataa tacgtaatat ggcggaaacc ttcggcttca cactgggtgc aaagcgcatc
     6601 gcctgactga taaagccctt ccagcgcggt attcgccgcc gggcttattt cattaatgat
     6661 cttaagcgta aaacgctccg gcaaattact gataaccagt gcgccctctt cttctttcca
     6721 ggcggtccac ggctcatcat taatatgaac agaaaccagt ttgaggtctt cgccgttgag
     6781 acgaagggga gcatctgatg caccatgacg gacagcctgg ctgaccgcgg taacgaccgt
     6841 cttttgcgcg tcgaggtcaa aggtcaagtc aatatcagta atctggtaat ccggcgcacg
     6901 ataatcgtga cggtatttgg cttgtggctg ttgagtcata aataaccttt agcatctttt
     6961 atagagtctg gtgttcagtc tattcctgtt gcgtaaatcg cgctatgcag aatcttcatc
     7021 ttttcaggta caaacgcctt tattgctaca tttttataac atacaccgcg taatgccatc
     7081 gaccagaaag gtggcatatg gtgtgatcgg ggttcaataa attgcgaaac aaggtatact
     7141 ccagcagttc ctgaagatgt ttattgtact aaacgctcct gtacgaggac gctactgcgc
     7201 acctatgaca caattcgctt ctcctgttct gcactcgttg ctggatacag atgcttataa
     7261 gttgcatatg cagcaagccg tgtttcatca ttattacgat gtgcatgtcg cggcggagtt
     7321 tcgttgccga ggtgacgatc tgctgggtat ttatgccgat gctattcgtg aacagattca
     7381 ggcgatgcag cacctgcgcc tgcaggatga tgaatatcag tggctttctg ccctgccttt
     7441 ctttaaggcc gactatctta actggttacg cgagttccgc tttaacccgg aacaagtcac
     7501 cgtgtccaac gataatggca agctggatat tcgtttaagc ggcccgtggc gtgaagtcat
     7561 cctctgggaa gttcctttgc tggcggttat cagtgaaatg gtacatcgct atcgctcacc
     7621 gcaggccgac gttgcgcaag ccctcgacac gctggaaagc aaattagtcg acttctcggc
     7681 gttaaccgcc ggtcttgata tgtcgcgctt ccatctgatg gattttggca cccgtcgccg
     7741 tttttctcgc gaagtacaag aaaccatcgt taagcgtctg caacaggaat cctggtttgt
     7801 gggcaccagc aactacgatc tggcgcgtcg gctttccctc acgccgatgg gaacacaggc
     7861 acacgaatgg ttccaggcac atcagcaaat cagcccggat ctagccaaca gccagcgagc
     7921 tgcacttgct gcctggctgg aagagtatcc cgaccaactt ggcattgcat taaccgactg
     7981 catcactatg gatgctttcc tgcgtgattt cggtgtcgag ttcgctagtc ggtatcaagg
     8041 cctgcgtcat gactctggcg acccggttga atggggtgaa aaagccattg cacattatga
     8101 aaagctggga attgatccac agagtaaaac gctggttttc tctgacaatc tggatttacg
     8161 caaagcggtt gagctatacc gccacttctc ttcccgcgtg caattaagtt ttggtattgg
     8221 gactcgcctg acctgcgata tcccccaggt aaaacccctg aatattgtga ttaagttggt
     8281 agagtgtaac ggtaaaccgg tggcgaaact ttctgacagc cctggcaaaa ctatctgcca
     8341 cgataaagcg tttgttcggg cgctgcgcaa agcgttcgac cttccgcata ttaaaaaagc
     8401 cagttaatat catcagggag ctaatcggct cccttttttt acctttaatt ccgaaatctt
     8461 tcgctgcatt tgcgaattct gcttgtctga ttgcagatgc caggtaacat aggtatcccc
     8521 ccatttacgg gtggacaagt gtttattttt tccgactatt aacagagaga atattatgag
     8581 cgttgtgcct gtagccgacg tactccaggg ccgcgtagcc gttgacagcg aagtcaccgt
     8641 acgcggatgg gtacgtaccc gccgagattc aaaagctggc atctccttcc tcgccgttta
     8701 tgacggttcc tgctttgatc ctgtacaggc tgtcatcaat aattctctgc ccaattacaa
     8761 tgaagacgtc ctgcgtctga ccaccggttg ctcggtcatt gtgacgggta aagtcgtggc
     8821 gtcgccgggc caggggcaac aatttgaaat tcaggccagc aaggttgaag ttgctggttg
     8881 ggttgaagat ccagacactt acccgatggc ggcaaaacgc cacagcattg agtatctgcg
     8941 tgaagtcgct cacctgcgtc cgcgcacaaa cctgattggt gccgccgcgc gcgttcgcca
     9001 tacgctggcg caggcgctgc atcgcttctt taacgagcag ggattcttct gggtttcaac
     9061 gccactgatt actgcgtctg ataccgaagg ggctggcgaa atgttccgcg tttctacgct
     9121 ggatctggaa aatctgccgc gtaacgatca gggcaaagtg gatttcgaca aagacttctt
     9181 tggtaaagag tctttcctga ccgtatctgg ccagttgaac ggcgaaacct acgcttgcgc
     9241 attatccaaa atttatacct tcggcccgac tttccgtgct gaaaactcca acaccagccg
     9301 tcacctggcg gagttctgga tgctggagcc ggaagtggcg tttgctaacc tgaacgatat
     9361 cgcgggtctg gctgaagcca tgctgaaata tgtcttcaaa gcggttctcg aagaacgcgc
     9421 agacgacatg aaattcttcg ctgaacgcgt agataaagat gccgtttcac gtctggaacg
     9481 cttcattgaa gccgattttg cgcaggtgga ttacaccgac gcagtgacca ttctcgaaaa
     9541 ctgcggcagg aagtttgaaa acccagttta ctggggcgtt gatctctctt ctgagcatga
     9601 gcgttatctg gcggaagaac actttaaagc accggtagtg gttaaaaact atccgaaaga
     9661 tattaaagcg ttctatatgc gccttaacga agacggtaaa accgttgcgg ctatggacgt
     9721 tctggctccg ggcatcggtg agatcattgg tggctcccag cgtgaagagc gtctggacgt
     9781 gctggacgag cgtatgctgg aaatgggcct gaacaaagaa gattactggt ggtatcgcga
     9841 tctgcgtcgc tacggtactg ttccgcattc cggtttcggt cttggttttg aacgcctgat
     9901 tgcttacgta actggcgtgc aaaacgtgcg tgatgtgatt ccgttcccac gtactccgcg
     9961 caacgccagc ttctgatttt cttcctcaaa gagccagcgc ctgctggctc ttttttatcc
    10021 tttctgtcaa gttatctgtt tgttaagtca agcaatctat ttgcaacccc gccataacga
    10081 tcatcctgtt acggaatatt acattgcaac atttacgcac aaaaaataac ctggattctt
    10141 attgcggatt agttttttct tagctgatag cacaattttc atactatttt ttggcattct
    10201 ggatgtctga acgaggattt agtgccaggt cgataaagtt tccatcagaa acaaaatttc
    10261 cgtttagtta atttaaatat aagga
//
LOCUS       DAPHBJ010000157        10246 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.159, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000157 DAPHBJ010000000
VERSION     DAPHBJ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.159"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(114..2687)
                     /gene="clpB"
                     /locus_tag="RHZ76_003473"
     CDS             complement(114..2687)
                     /gene="clpB"
                     /locus_tag="RHZ76_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDV2814061.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2817..3548)
                     /gene="pgeF"
                     /locus_tag="RHZ76_003474"
     CDS             complement(2817..3548)
                     /gene="pgeF"
                     /locus_tag="RHZ76_003474"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HDV2814062.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAADAKASTAFIQHGDKYLADIYLLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3545..4525)
                     /gene="rluD"
                     /locus_tag="RHZ76_003475"
     CDS             complement(3545..4525)
                     /gene="rluD"
                     /locus_tag="RHZ76_003475"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HDV2814063.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIIINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4660..5397
                     /gene="bamD"
                     /locus_tag="RHZ76_003476"
     CDS             4660..5397
                     /gene="bamD"
                     /locus_tag="RHZ76_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HDV2814064.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5668..6009
                     /gene="raiA"
                     /locus_tag="RHZ76_003477"
     CDS             5668..6009
                     /gene="raiA"
                     /locus_tag="RHZ76_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDV2814065.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6112..6236
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6113..6160
                     /gene="pheL"
                     /locus_tag="RHZ76_003478"
     CDS             6113..6160
                     /gene="pheL"
                     /locus_tag="RHZ76_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HDV2814066.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6258..7418
                     /gene="pheA"
                     /locus_tag="RHZ76_003479"
     CDS             6258..7418
                     /gene="pheA"
                     /locus_tag="RHZ76_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HDV2814067.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7461..8582)
                     /gene="tyrA"
                     /locus_tag="RHZ76_003480"
     CDS             complement(7461..8582)
                     /gene="tyrA"
                     /locus_tag="RHZ76_003480"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HDV2814068.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8593..9663)
                     /gene="aroF"
                     /locus_tag="RHZ76_003481"
     CDS             complement(8593..9663)
                     /gene="aroF"
                     /locus_tag="RHZ76_003481"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HDV2814069.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9873..10238
                     /locus_tag="RHZ76_003482"
     CDS             9873..10238
                     /locus_tag="RHZ76_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HDV2814070.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
BASE COUNT         2632 a         2721 c         2388 g         2505 t
ORIGIN      
        1 gaaaaatata aaaactttat cgttcgctca tttttcaggc aaaacatctt aaatatagtc
       61 ttttccgtct aacttataga caaaaacgag ccccgaaggg ctcgttttat catttactgg
      121 acggcgacaa tccggtcttc attaacttcc aggcgaatca ctttacccgg aaccaattca
      181 ccagacagta tttgctgtgc cagcgggttt tcgatctgct gctggatagc acgtttcaaa
      241 ggacgtgcac catagactgg atcgtaaccg ttctcgctca gcagtttcag cgcctcgtca
      301 gaaatgtgga tttcataacc acgttcttcc agacgtttgt acagacgttt caactgaatc
      361 tgcgcaatcg aggcaatgtg ctgttcaccc agcggatgga agaccaccac ttcatcaata
      421 cggttaatga attccggacg gaagttatgg cttaccacac cgagcaccag ctctttcatg
      481 tgcgcataat ccagttcacc gaagcgttcc tgaatcaaat cggaaccgag gttagaggtc
      541 ataatgacaa ccgtattacg gaagtcgacc gttctcccct gcccgtcagt cagacgacca
      601 tcatccagta cctgcaacag aatgttgaag acatccggat gcgctttttc cacttcatcc
      661 agcaggatga cggaatacgg acgacgacgc acagcttcgg tcaggtagcc accttcttca
      721 taaccgacat atcccggagg cgcaccaacc aaacgagaca ccgagtgttt ctccataaac
      781 tcggacatat ctatacggac catcgcctcg tcgctatcaa acataaagtt cgccagcgcc
      841 ttacaaagct ctgttttccc cacacccgtt gggccgagga acaggaatga accaatcggg
      901 cgatttggat ccgccaaccc cgcacggcta cggcgaatag cgttagatac cgcatcaacc
      961 gcttcgttct gaccaatgac gcgatggtgc agttcttgct ccatacgcag cagtttttcg
     1021 cgctcgcttt ccatcatgcg agaaaccgga atccccgtcc aacgcgccag cacttcagca
     1081 atttcggcgt cggtcacttt attacgcaac agacgcatag ttttgccttc gagctgcgtt
     1141 gcggcttcca gttgcttttc cagttccggg attttgccgt attgcagttc agacatccgc
     1201 gccaggtccc ccacacggcg agcctgttca atagcgattt tcgcctgttc cagttccgct
     1261 ttaatggtct gcgtaccaga aagcgatgcc ttctctgctt tccactcttc ttctaactcg
     1321 gagtactgac gttctttgtc gctcagttct tcgttgagca tatccaggcg ttttttactg
     1381 gcttcatcag actctttcat taacgcctgt tgttccagtt tgagctggat gatacgacga
     1441 tcgagtcggt cgagttcttc tggttttgag tcaatctgca tacgaatgct ggatgctgct
     1501 tcatcgatca ggtcgatggc tttatccggc agctgacggt cagcaatgta gcgatgagac
     1561 aacgtcgccg ctgcaacaat tgccgggtca gtaatttgca catggtggtg caattcgtaa
     1621 cgttctttca ggccacgcag aatcgcaatg gtatcttcaa cagaaggctc ggcaacaaac
     1681 actttctgga aacgacgttc cagcgcagca tctttttcga tgtactggcg atattcgtca
     1741 agcgtcgtgg cacctacgca gtgcaattca ccacgcgcca gcgccggttt cagcatgttt
     1801 ccggcgtcca ttgcgccatc ggctttaccc gcgccgacca tggtatgtaa ttcgtcgata
     1861 aacaggatga cgttgccttc ctgtttggca agatcgttaa gtacgccttt taaacgttct
     1921 tcaaactcac cgcgatattt cgccccagcc accagcgcgc ccatatccag cgccagtacc
     1981 cggcggcctt tcaacccttc cggcacttcg ccgttgataa tacgctgcgc cagaccttca
     2041 acgatggcag ttttaccgac gccgggttca ccaatcagta ccgggttatt tttagtacga
     2101 cgttgcagca cctgaatggt acggcgaatt tcttcatcac gaccaatcac cggatcgagt
     2161 ttgccctgtt cggctcgttc ggtaaggtcg atggtatatt ttttcaaagc ctgacgttgg
     2221 tcttcagcac cttgatcgtt cacgctttca cctccacgca tttgttcaat cgcttgagta
     2281 atgttggcgg tggtcgcccc tgctgctttc aggatgtcgg ccagcgtgcc gcgagactca
     2341 agtgccgcca gaacgaacag ttctgacgag ataaagttat caccacgttt ttgcgccagc
     2401 ttgtcgcaaa gattaagaac gcgcaccaga tcctgtgatg gctggacatc accaccagta
     2461 ccttcaacct gcggtaaacg atttaatgcc tgattgatat ctgtgcgcaa ctggccagca
     2521 tttatgccag ctgatgttaa caaaggacta accgaacccc cttcctgatt cagcagggcg
     2581 ctcattaaat gaagtggttc gatgaattgg ttgtcgtgcc cgagtgcaag tgattgggca
     2641 tcggcaagag caagctggaa tttattagta agacgatcca gacgcataac tcctcccata
     2701 acggatcaaa gttgctactg gagattaaat gaggtcatcc ctcaattatt caaggttatt
     2761 gaccagatta atgtgaaaag aaaatcacgc gtaccggatc gtcttgattc tttaggttat
     2821 atcagccaaa tgaaacttgc catacgcccg gtggtcttgt cgcgacgata agagaagaaa
     2881 gtctcatttt ccgtatatgt acaacggtcg ccgccgaaaa tttgttcaac accaacgctc
     2941 gccagacgct gccgggcaag cagataaata tccgccagat acttatcacc atgctgaatg
     3001 aaagctgtac tcgctttggc gtctgctgcc ataaacgcct cgcgaacctc cgcccccact
     3061 tcgaacgcgc gtggaccaat tgccggccct aaccaggcga gaatattttc cggattatca
     3121 gcaaaacagg aaactgtctc ttccagcacg cctgcgcaca gtccacgcca gccagcatga
     3181 gcggcggcga cttccgttcc cgctcgattg caaaacagca cagggaggca gtcggcagtc
     3241 atcactgcgc aaaccgtgcc aggcgtattg ctataagagg catctgctcg ttttgaggca
     3301 taaggttcgc cagtaagctt aagcacatct ttgccgtgta cctgctcaag ccagaccggt
     3361 ttagaaggca aattgcccgc agcaaaaagt cgcttgcgat tctcctcaac gtgatccggg
     3421 ttatcgccac aatgggcacc gaggttgagc gagtcatacg ggggcaagct cacgccgccg
     3481 atacgagtag agctacaagc cgcaacaccc tttggcagcg gccactgcgg gacaatcagc
     3541 ttactcataa ccagtccact tcatccttat gttcttcgaa atcggcgcgc atcacctcaa
     3601 tcagctccac catatcttgt ggaataggcg catgccattc catttcgatg ccggagatcg
     3661 ggtgataaag acgcagcatg gttgcatgta gcgcctggcg gtcaaactta cgcagcgtgg
     3721 agataaatgc ttccgaagca ccttttggcg gacgcggacg gccaccataa accggatcgc
     3781 ccaccagcgg atgagtgata tgggccatat gcacgcggat ctggtgcgta cgtccagttt
     3841 ccagacgcaa ccgcagacgc gtgtgcacac ggaagtgttc catgatgcga tagtgagtca
     3901 ccgctggttt gcccatcgga tgcaccgcca tatgggtacg tttggtcggg tggcgactga
     3961 ttggctcgtc caccgtgcca cctgcggtca tatgaccaat cgccaccgct tcatactcac
     4021 gagtaatttc acgccgttgc aaagattcga ctaaacgcgt ctgagccgga acggtttttg
     4081 ccacaaccat caggccagtg gtgtctttat ccagacgatg gacgatgccc gcacgcggta
     4141 catcggcaat gggtggatag taatgaagca acgcattcag taccgtgcca tccgggttac
     4201 ccgcgccagg atgtaccacc aggtcgcgcg gtttattaat gataataatg tcttcatctt
     4261 catagacgat atccagcggg atatcctgcg gttcaaaacg cgcttcttct tcaatctcag
     4321 cgttgatggc aacctgctcg ccacccaata ctttttcttt cggcttatca caaactttgc
     4381 cgttaaccag cactcgctgg tcgaggatcc attcttttat tcgcgaacgt gaataatccg
     4441 ggaacatttc ggccaaagcc tgatctaagc gttgaccgag ttggttttcg gacaccgttg
     4501 cagtgagctg tactcgttgt gccatatata ctgcttcttc gtttatcgtt ggggttttac
     4561 ggctttgccg tttaatatag tgtgctattg tagctggtct taaccgggag caggaacaga
     4621 gaatctcccg tattacattt tgaggaaagt caaaacgtca tgacgcgcat gaaatatctg
     4681 gtggcagccg ccacactaag cctgtttttg gcgggttgct cggggtcaaa ggaagaagta
     4741 cctgataatc cgccaaatga aatttacgcg actgcacaac aaaagctgca ggacggtaac
     4801 tggagacagg caataacgca actggaagcg ttagataatc gctatccgtt tggtccgtat
     4861 tcgcagcagg tgcagctgga tctcatctac gcctactata aaaacgccga tttgccgtta
     4921 gcacaggctg ccatcgatcg ttttattcgc cttaacccga cccatccgaa tatcgattat
     4981 gtcatgtaca tgcgtggcct gaccaatatg gcgctggatg acagtgcgct gcaagggttc
     5041 tttggcgtcg atcgtagcga tcgcgatcct caacatgcac gagctgcgtt tagtgacttt
     5101 tccaaactgg tgcgcggcta tccgaacagt cagtacacca ccgatgccac caaacgtctg
     5161 gtattcctga aagatcgtct ggcgaaatat gaatactccg tggccgagta ctatacagaa
     5221 cgtggcgcat gggttgccgt cgttaaccgc gtagaaggca tgttgcgcga ctacccggat
     5281 acccaggcta cgcgtgatgc gctgccgctg atggaaaatg cataccgtca gatgcagatg
     5341 aatgcgcaag ctgaaaaagt agcgaaaatc atcgccgcaa acagcagcaa tacataacag
     5401 aaacctgaaa cacaaaacgg cagcccttga gctgccgttt ttttattctg tcagttgtga
     5461 aactgaagcg atttagtcgc tatcgatctc atcaaatatg gctcgctttg agatattcct
     5521 caagtaaaaa aacacctctt cctgcgattt ctcacaaaaa agattcgttg acaaaaagtg
     5581 acaaaattat gagattttca tcacacattt tgacatcagg aacggtatgc tgaattcacc
     5641 aagacgggaa gacaagaggt aaaatttatg acaatgaaca ttaccagcaa acaaatggaa
     5701 attactccgg ccatccgcca acatgtcgca gaccgtctcg ccaaactgga aaaatggcaa
     5761 acacatctga ttaatccaca tatcattctg tccaaagagc cacaagggtt tgttgctgac
     5821 gccacaatca atacacctaa cggcgttctg gttgccagtg gtaaacatga agatatgtac
     5881 accgcaatta acgaattgat caacaagctg gaacggcagc tcaataaact gcagcacaaa
     5941 ggcgaagcac gtcgtgccgc aacatcggtg aaagacgcca acttcgtcga agaagttgaa
     6001 gaagagtagt cctttatatt gagtgtatcg ccaacgcgcc ttcgggcgcg ttttttgttg
     6061 acagcgtgaa aacagtacgg gtactgtact aaagtcactt aaggaaacaa acatgaaaca
     6121 cacaccgttt ttcttcgcat tcttttttac cttcccctga aagggaggcg tttcgtcgtg
     6181 tgaaacagaa tgcgaagacg aacaataagg cctcccaaat cgggggcctt ttttattgat
     6241 aacaaaaagg caacactatg acatcggaaa acccgttact ggcgctgcga gagaaaatca
     6301 gcgcgctgga tgaaaaatta ttagcattac tcgcagagcg gcgcgaactg gccgtcgagg
     6361 tgggaaaagc caaactgctc tcgcatcgcc cggtacgtga tattgatcgt gaacgcgatt
     6421 tactggaaag attaattacg ctcggtaaag cgcaccatct ggacgcccat tacattactc
     6481 gcctgttcca gctcatcatt gaagattccg tattaactca gcaggctttg ctccaacaac
     6541 atctcaataa aattaatccg cactcagcac gcatcgcttt tctcggcccc aaaggctcct
     6601 attcacatct tgccgctcgt cagtacgctg cccgtcactt tgagcaattc attgaaagtg
     6661 gctgcgccaa atttgccgat atttttaatc aggtggaaac cggccaggcc gactatgccg
     6721 tcgtaccgat tgaaaatacc agctccggtg ccataaacga cgtttacgat ctgctgcaac
     6781 ataccagctt gtcgattgtt ggcgagatga cgttaactat cgaccattgt ttgttggtct
     6841 ccggcactac tgatttatcc accatcaata cggtctacag ccatccgcag ccattccagc
     6901 aatgcagcaa attccttaat cgttatccgc actggaagat tgaatatacc gaaagtacgt
     6961 ctgcggcaat ggaaaaggtt gcacaggcaa aatcaccgca tgttgctgcg ttgggaagcg
     7021 aagctggcgg cactttgtac ggtttgcagg tactggagcg tattgaagca aatcagcgac
     7081 aaaacttcac ccgatttgtg gtgttggcgc gtaaagccat taacgtgtct gatcaggttc
     7141 cggcgaaaac cacgttgtta atggcgaccg ggcaacaagc cggtgcgctg gttgaagcgt
     7201 tgctggtact gcgcaaccac aatctgatta tgacccgtct ggaatcacgc ccgattcacg
     7261 gtaatccatg ggaagagatg ttctatctgg atattcaggc caatcttgaa tcagcggaaa
     7321 tgcaaaaagc attgaaagag ttaggggaaa ttacccgttc aatgaaggta ttgggctgtt
     7381 acccaagtga gaacgtagtg cctgttgatc caacctgatg aaaaggtgcc ggatgatgtg
     7441 aatcatccgg cactggatta ttactggcgg ttgtcattcg cctgacgcaa taacacgcgg
     7501 ctttcactct gaaaacgctg tgcgtaatcg ccgaaccagt gctccacctt gcggaaactg
     7561 tcaataaacg cctgcttatc gccctgctcc agcaactcaa tcgcctcgcc gaaacgctta
     7621 tagtaacgtt tgattaacgc cagattacgc tctgacgaca taataatgtc ggcataaagc
     7681 tgcggatcct gagcaaacag tcgcccgacc atcgccagct caaggcggta aatcggcgaa
     7741 gagagcgcca gaagttgctc aagctgaaca ttttcttctg ccagatgcag cccataagca
     7801 aaagtagcaa agtggcgcag agcctgaata aacgccatat tctgatcgtg ctcgacagcg
     7861 ctaatacgat gcagccgagc gccccagacc tgaatttgct ccagaaacca ttggtatgct
     7921 tccggcttac gtccatcaca ccagaccaca acttgctttg ccaggctacc gctgtccggg
     7981 ccgaacatcg ggtgtaaccc cagtaccggg ccatcgtgcg ccgccagcat ggcctgtaat
     8041 ggtccatttt tcactgatgc cagatcaacc agaatacaat ctttcggtaa aggcggtaat
     8101 ttgccgataa cttgctcagt aacgtggatt ggcacactaa caatcaccat tccggcatcg
     8161 gcaacaatat cagccgctcg atcccagtca tgttgctcca gaatccgcac ctgataaccc
     8221 gatagtgtca gcatcttctc gaacaggcgt cccatctgac cgccaccgcc gacgataacc
     8281 accggacgca gtgacggaca aagtgtttta aatcctttgt cgttttcact ggagtaagat
     8341 tcacgcatca cccgacgcaa aacatcctca atcagatctg gcggtacacc cagagcttcc
     8401 gcctctgcgc gccgcgaggc caacatagat gcctcgcgct ccggaacata aataggcagt
     8461 ccaaagcggc ttttcacctc gcccacttca gcaaccagtt ccagacgctt cgctaataaa
     8521 ttcagcagcg ctttatcgac ttcatcaatt tgatcgcgta atgcggtcaa ttcagcaacc
     8581 ataataaacc tcttaagcca cgcgagccgt cagctgcccg ttcagatcct gatgaatttc
     8641 acgcagcaag gcatcggtca tttcccagct aatgcaggca tcggttacgg atacaccgta
     8701 tttcatttca ctgcgcggtt gctcggaaga ctgattgccc tcgtggatat tactttcgat
     8761 catcagacca ataattgagc gattgccatc tttgatttga gcaaccacgg attctgccac
     8821 cgcaggctga cggcgataat ctttattgga attaccgtgg ctgcaatcta ccatcagaga
     8881 cgggcgcagt cccgcctgtt ccatctcttt ttcacattgc gcaacatccg cagggctata
     8941 gttcggcgct ttaccaccgc gcaggatcac atggccgtcc ggattcccct gagtttgtag
     9001 caacgcaacc tgccctgcct ggttaatgcc aacaaaacgg tgcggctggg cggcggcgcg
     9061 catagcgtta attgctgttg ccagactgcc gtcggtgccg tttttaaaac caaccggcat
     9121 ggaaagcccg gaggccattt cacggtgagt ttgcgattcc gttgtacgag caccaattgc
     9181 tgaccagcta aacagatcgc ccaggtattg cgggctattc ggatctaacg cttccgtcgc
     9241 cagtggcagt cccatattca ccagctcaag cagcaattta cgcgcgatct gcagcccggc
     9301 ttctacatca aaagagccat ccatatgggg atcgttaatt aaccctttcc agccgacagt
     9361 ggtacggggt ttttcaaaat agacgcgcat taccagatag aggctatcgc tgacctctgc
     9421 ggcaagggct ttaaatcgac gagcatattc cagagcagtt tccggatcat gaatggaaca
     9481 aggaccacat actaccagca gacgaggatc gcgcccggcg ataatatctg aaatggtttt
     9541 acgcgagtca gcaatctggg cttcttgttg caggctcaat ggaaaagcgg ccttcagttg
     9601 ttccggagtc attaaaacct gttcgtcggt aatatgtacg ttattcagcg cgtctttttg
     9661 catgatggcg atcctgttta tgctcgtttg cgatagttga tcctcagcga ggatgacgta
     9721 acgataacac ataaagtaaa gttttcaatc catatttcgt acatttttat ttacacaggc
     9781 aatttagtcg cgatttcaac ccttacttct gtatagataa atttacactc cctttgaaaa
     9841 caatccgcta tgctttgaaa aaggagaaag aaatgatgaa aaagtttatc gcccccttgt
     9901 tggctttact ggttagcgga tgtcagattg atccttatac tcacgcgcca accttgacca
     9961 gcaccgactg gtatgatgtt ggtatggaag atgcgatatc gggcagcgcc ataaaagatg
    10021 acgatgcatt tagcgattca caggcggatc gcggtctata ccttaaagga tatgccgaag
    10081 gacaaaagaa aacttgccag accgatttta cttatgcccg aggactttcc ggtaaaagct
    10141 ttcctgccag ctgcaacaat gttgaaaatg ccagccaact gcatgaagta tggcaaaaag
    10201 gggctgatga aaatgccagc acaatacgat taaattaatt aaaacc
//
LOCUS       DAPHBJ010000158        10195 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.160, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000158 DAPHBJ010000000
VERSION     DAPHBJ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.160"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(58..1248)
                     /gene="bcr"
                     /locus_tag="RHZ76_003483"
     CDS             complement(58..1248)
                     /gene="bcr"
                     /locus_tag="RHZ76_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HDV2814071.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIEQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(1276..1971)
                     /gene="rsuA"
                     /locus_tag="RHZ76_003484"
     CDS             complement(1276..1971)
                     /gene="rsuA"
                     /locus_tag="RHZ76_003484"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HDV2814072.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            2120..3880
                     /locus_tag="RHZ76_003485"
     CDS             2120..3880
                     /locus_tag="RHZ76_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDV2814073.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRLAHELRG"
     gene            4005..4289
                     /gene="rplY"
                     /locus_tag="RHZ76_003486"
     CDS             4005..4289
                     /gene="rplY"
                     /locus_tag="RHZ76_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HDV2814074.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(4428..5435)
                     /gene="yejK"
                     /locus_tag="RHZ76_003487"
     CDS             complement(4428..5435)
                     /gene="yejK"
                     /locus_tag="RHZ76_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HDV2814075.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELKSLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            5617..5844
                     /locus_tag="RHZ76_003488"
     CDS             5617..5844
                     /locus_tag="RHZ76_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HDV2814076.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            5864..7624
                     /gene="pbgA"
                     /locus_tag="RHZ76_003489"
     CDS             5864..7624
                     /gene="pbgA"
                     /locus_tag="RHZ76_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HDV2814077.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            7699..7775
                     /locus_tag="RHZ76_003490"
     tRNA            7699..7775
                     /locus_tag="RHZ76_003490"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7733..7735,aa:Pro,seq:ggg)
     gene            complement(7878..>10195)
                     /gene="yejO"
                     /locus_tag="RHZ76_003491"
     CDS             complement(7878..>10195)
                     /gene="yejO"
                     /locus_tag="RHZ76_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HDV2814078.1"
                     /translation="YSVLNDGYQIVQMGGAANQTTLNNGVLQVYGAANDTTIKGGRLI
                     VEKDGGAVFVAIEKGGLLEVKEGGFAFAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIK
                     NGIANNMLLENGGSLRVEENDFAYNTTVDSGGLLEVMDGGTVTGVDKKAGGKLIVSTN
                     ALEVSGPNSKGQFSIKDGVSKNYALDDGSGLIVMEDTQAIDTILDKHATMQSLGKDTG
                     TKVQANAVYDLGRSYQNGSITYSSKAISENMVINNGRANVWAGTMVNVSVRGNDGILE
                     VMKPQINYAPAMLVGKVVVSEGASFRTHGAVDTSKADVSLENSVWTIIADITTTNQNT
                     LLNLANLAMSDANVIMMDEPVTRSSVTASAENFITLTTNTLSGNGNFYMRTDMANHQS
                     DQLNVTGQATGDFKIFVTYTGASPAAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEY
                     TLLDNGNHSWSLAENRAQITPSTTDVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDT
                     AMWSSAINTRNNVTTDAGAGFEQTLTGLTLGIDSRFSREESSTIRGLIFGYSHSDIGF
                     DRGGKGNIDSYTLGAYAGWEHQNGAYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNG
                     AGAHVESGFRWVDGLWSVRPYLAFTGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAV
                     SYHMDLQNGTTLEPWLKAAVRQEYADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFT
                     PTLSGHLSVSYGNGAGVESPWNTQAGVVWTF"
BASE COUNT         2551 a         2759 c         2431 g         2454 t
ORIGIN      
        1 ttgtggttga catgcatcaa caaaggaagc cttttagctt cctcgttgtg caatagatca
       61 ccgttttttc ggccgactgg cgtacagaca gaagagaatg gagctggttg cgcagaatgc
      121 aattgaccaa atcatcggcc atgcagagtt aaaggtcgca agagaaagca atgcgccaac
      181 aattgcccct atgccaaaac ggaaggttcc tgccagcgaa gatgccgttc ccgccatatg
      241 gggaaactca tcaagaatga ccgccatcgc attggatgac accatcgaca cgcagcccac
      301 aaacgccgca acgccaacca ccagcgacca aaatcccagc cccagcagcg cactgatgac
      361 catccacgct gccataataa attgtatcca caaccccgag cggaacatat ttaacgcgcc
      421 aatgcggcgg acgaagcggc tgttaaagat ggtcatcacg aacagaaaaa caatgtttag
      481 cgcaaagtaa taaccaaagt tttccggcgc gacgtggtta atttcaatat aaacaaacgg
      541 tccggcgctt aagaatgaga acatcccggc aaagctgaaa ccactggcaa gcatgtagct
      601 caggacacgt ttatggcgga acaacgccgc aaagttacca atagtggtac gaatgtgaaa
      661 tggctgacga cgctccggtg gtaaggtttc tttaatcagg aagaaaatca ttgccgaagc
      721 cagaatcgcc gctaatgcca ggatccagaa gatgtaatgc cagctcagcc acaccagcac
      781 ccagccgcca actatcggtg ccatcagcgg tgcaatggtt gtcaccagca tgacaaacga
      841 catcatccgc gagaactctt ctttcgggta aatatcgcgc atcagggcgt taatgaccac
      901 gctggccgca gccgcagcca gcccgtggaa gaaacgcatc acaatcagct gctcgatggt
      961 gtttgccaac gcacacgcca ccgcggcggc ggcaaacacc agcgtaccgc cgagcaccac
     1021 cggcttacgc ccgaagctgt ctgccatcgg cccgtagatt aactgcccca acgcaaagcc
     1081 cagaatataa gtactgaggg tcatctgcgt actgcccgcc ggtacgccaa actgcgctga
     1141 aattaccggt agcgcgggca gatacatatc aatcgacagc ggcatcaaca tggccagcag
     1201 gccaaggata aaaacaatag caaacgacga atgctgtcgg gtggtcacaa cgggctcctg
     1261 aaagtcattg aaaagttaga cgacgctggc aatttcttct tcagttaacg gacgatattc
     1321 accgggggct aaatcagcat ccagcgtaat accgccaata cgttcacgat gcagctcaac
     1381 cacgtggtta cccacggcgg cgaacatgcg tttcacctga tgataacgcc cttcgctgat
     1441 ggtcagacga acctgcgttg gggtaatcac ttccagcacc gcaggcttag tgagatcttt
     1501 ttcgttatgc agctgcacgc ctttagcaaa ttgctctgcc gtatcgtcag ctacaggtga
     1561 ttccagtgtc accagatagg tcttctcgca atgatggcgc ggagaagtaa tgcggtgcga
     1621 ccactgacca tcatcagtca tcagcaccag accggtggta tcaatatcca accgccccgc
     1681 cgcatgcagt ttccacgcta ccggttcatc aagaaaatag agcaccgttg ggtgatcagg
     1741 gtcgtccgtg gagcaaacat agccctgagg cttattgagc atgaagtaac gtggaccgtg
     1801 ttgctgcgcc agcgggttgc catcgtaagc gacatcatgt tcaggaagca gtttgaacgc
     1861 tgcattacgg acgatttcgc catcgacggt gacacgattg ccgcggattt cacgcccggc
     1921 aatagcacgg ctaacgccga gttgctgtgc gataaattta tcaagtcgca tgtgtgtgat
     1981 tttgcctgta aaaacggagg tcgggcattg acccgaaaat ctgaactgtt gtctgcccag
     2041 tatagcggtc taataacatc cctcaaggga aaaagatcca tggcatacta ttagcagaat
     2101 aatctaccta cgcgagacca tgatttttac acttcgccca tatcagcaag aagccgtgga
     2161 tgccacgctc aaccattttc gtcgtcataa aacccctgcc gttatcgtgc tgcccaccgg
     2221 cgcaggtaaa agcctggtga tagcggaact ggcacggcta gctcgtggtc gcgtgctggt
     2281 gctggcacac gttaaagaac tggtggcgca aaaccatgca aagtatcagg cgctggggct
     2341 ggaagccgat atttttgccg ccgggctaaa gcgcaaagag agccacggta aagtggtatt
     2401 tggcagcgtg cagtctgtcg cccgtaatct tgatgccttt cagggtgaat tttcgctgtt
     2461 gattgtcgat gaatgtcacc gtattggtga cgatgaagag agccagtatc agcaaatcct
     2521 cactcacctg acaaaagtga atccccactt acgcctgctg gggctgactg ccacgccttt
     2581 tcgattgggc aaaggctgga tctaccagtt tcattatcac ggcatggtac gcggcgatga
     2641 gaaagccctt ttccgtgact gcatttatga gctgccgctg cgttatatga ttaaacacgg
     2701 ctatctgacg ccgccagaac gactggatat gccagtagtg caatacgatt tcagccgctt
     2761 gcaggcacag agtaacgggc tgttcagcga agccgatctc aaccgtgagc tgaaaaaaca
     2821 acaacgtatt accccgcaca tcatcagcca gattatggag tttgctgcaa cgcgcaaagg
     2881 ggtgatgatt tttgccgcga cggttgaaca cgcaaaagag attgtgggat tactgcctgc
     2941 cgaagatgca gcactgatta ctggcgacac ccccggcgct gagcgcgatg tgttaattga
     3001 aaattttaaa gcccagcgtt ttcgctatct ggtcaacgtc gcggtactga ccaccggatt
     3061 tgacgccccg cacgtcgatc ttatcgccat tctgcgccct accgaatcag tgagtcttta
     3121 ccaacaaatt gtcgggcgcg gtctgcgtct cgctccgggc aagactgatt gcttaattct
     3181 tgattatgcg ggtaatcctc acgatctcta cgcgccggaa gttggtacac caaaaggcaa
     3241 aagtgacaac gttccggtac aggttttctg ccctgcctgc ggttttgcca acaccttttg
     3301 ggggaaaacg accgccgacg ggacattgat tgaacacttt ggtcgtcgct gtcagggatg
     3361 gtttgaagat gacgacggtc atcgcgaaca atgtgacttc cgtttccgtt ttaaaaattg
     3421 cccgcaatgt aacgcggaaa acgatattgc cgcccgccgc tgccgcgaat gtgacaccgt
     3481 actggttgat ccggacgata tgttaaaagc ggcgctacga ctgaaagacg cgctggtatt
     3541 acgctgtagc ggcatgtctt tgcaacatgg gcacgacgag aaaggcgaat ggttgaaaat
     3601 cacctattac gatgaagacg gcgcggatgt gagtgagcgt ttccgtctgc aaacacctgc
     3661 ccagcgtacc gccttcgagc agctttttat ccgcccgcat acgcgcacac cgggcatccc
     3721 gctgcgctgg atcaccgccg ccgatatcct cgcccagcaa gccttattgc gacacccgga
     3781 ttttgtcgtc gcccgcatga aaggccagta ctggcaggtg cgtgaaaaag tgttcgatta
     3841 cgaaggtcgt tttcgtctgg cgcacgaatt acgcggttaa tggtagtttt gattgatgta
     3901 taaggcgatt gagtatagaa tctcgcccgc ttttgcatac gcaaagcaga tcacttacct
     3961 gttgctgggt cgcctgtagc aggattaatt taagagagaa agaaatgttt actatcaacg
     4021 cagaagtacg taaagagcag ggtaagggtg cgagccgccg cctgcgtgcc gctaacaagt
     4081 tcccggcaat catctacggt ggcaaagaag cgccgctggc tatcgagctg gatcacgaca
     4141 aagtcatgaa catgcaagct aaagctgaat tctacagcga agttctgacc atcgttgttg
     4201 acggtaaaga aatcaaagtt aaagctcagg acgtacagcg tcacccgtac aaaccgaagc
     4261 tgcagcacat cgacttcgtt cgcgcttaat tgctgaataa gttgtaaaaa accccgctcc
     4321 ggcggggttt tttgtatctg cagattatgc ctgattacgg tattgctatt ttttgcagac
     4381 cagataaggc gttcacgccg catctggcat tcagaacaca aaaccgatta attgccgcca
     4441 gacgtccggc gctgcaattg gtcgcgcaaa ttcggcggcg tgcctttaat ggtcaaggta
     4501 tcggttgctg gatcccagaa gatgcgttca cctaacagca tcgcatcaaa gttaatagtc
     4561 aagccaccgc cgctaccggc aaacttcgtc aactgacgca gtgtgctacg gtctgccggg
     4621 aagctctctt ccagttcgta gcctttttca gccgcaaact ccgtgaagct cacttcgctt
     4681 acgcctgcca gctctttcga cagcgatttc agttcgatct cttcgcctgc ctgcagctgc
     4741 tcattacagt agctataaac ctgctggcgc acgttctgtc gctctgcttt atccagttgt
     4801 gcttcggcag tgaaatcatc aaccgcttgc aacagcccac ggttttgcgc tttcgcgttt
     4861 aacccttcgc tggccccgag gaaatccata aagaaatccg ccactttgcg ccctactcgc
     4921 cctttcagga aagtgagata gcgggtggac tctggattgg tttcccattc ggttaaatcg
     4981 atacgcgcaa caatatccgc atgattgata tcaagataat gggttgggtt gatgtcgaga
     5041 ttttcgttaa cgcgcatact gctcaggttg ctcagaactg ccaccagcaa atactccacc
     5101 gccagatagc gatagtggca aaacagcacg aatccaccgt cagcaaaagg atatttcgct
     5161 aattcgtcac gcaggcggcc ggttgccgcg cggctaaatg ccaggaagtc ctcttcgccc
     5221 tgacgctgta agcgcagcgt ctgcgccagt tcactctctt cgctaaacaa gccgtaagct
     5281 ttatttttgg cgctatagac ccgatgcagt tctgccacca tctcaacgac ggtttctgtc
     5341 ggttccagca atgaatcgcg caacaccagc tcaaggttct gctcatcacg cttgataagc
     5401 tggtgcagag caatctggtt gatatccaga ctcatgataa actctccttt aagaccgggc
     5461 ggtattcaac caccgccggt taagcgacgc aataaaagat ggcggtcatt ttacagaagg
     5521 cagcgtaacg agaatgattt aacgcataaa aaagcagaaa aaaaaccgtt gctacggtaa
     5581 tatgttgccc tttcatcaac taactgattt cgatttatgc cacaaatttc ccgctacagt
     5641 gatgaacagg ttgaacaatt gctcgcagag ctgctcaacg tactggaaaa acataaggct
     5701 ccgaccgacc tttccctgat ggtgttaggc aacatggtca ctaaccttat caacaccagc
     5761 attgccccgg cccaacgcca ggcaattgcc aactcttttg cccgcgcctt acagtcctct
     5821 atcaacgaag acaaagcgca ctaagggaaa cagataacag gttatggtaa ctcatcgtca
     5881 gcgctaccgt gaaaaagtct cccagatggt cagttggggg cactggtttg cactgttcaa
     5941 tattctgctt tcgctcgtca ttggcagccg ttacctgttt atcgccgact ggccgacaac
     6001 gcttgctggt cgcatttatt cctacgtaag cattatcggc catttcagct tcctggtgtt
     6061 cgccacctac ttgctgatcc tcttcccgct gacctttatc gtcggctccc agaggctgat
     6121 gaggtttttg tccgtcattc tggcaacggc gggaatgacg ctattactga tcgatagcga
     6181 agtctttact cgtttccatc tccatcttaa tcccatcgtc tggcaactgg ttatcaaccc
     6241 agacgaaaat gagatggcgc gcgactggca gctgatgttc atcagcgtgc cggttatttt
     6301 attgcttgaa ctggtgtttg cgacgtggag ctggcaaaag ctgcgcagcc tgacgcgtcg
     6361 tcgacgcttc gcgcgcccgc tggccgcatt cttatttatc gcctttatcg cctcgcatgt
     6421 ggtgtatatc tgggccgatg ccaacttcta tcgcccgatc accatgcagc gcgctaacct
     6481 gccgctttcg tacccgatga cggcgcgacg ttttcttgag aagcatggtc tgcttgatgc
     6541 gcaggagtat caacgccgtc ttattgagca aggtaatcca gacgccgttt ccgttcagta
     6601 tccgttaagc gaactgcgct atcgcgatat gggcaccggg cagaatgtgc tgttgattac
     6661 tgtcgatggc ctgaactact cacgcttcga gaagcagatg cctgcgctgg caggttttgc
     6721 tgagcaaaat atttcgttca cgcgccatat gagctccggc aacactacag acaacggcat
     6781 ctttggcctg ttctatggca tctcgccgag ctatatggac ggcattctgt cgacccgtac
     6841 gcctgcggca ttaattactg cgcttaatca gcaaggctat cagctggggt tattctcatc
     6901 agatggcttt accagcccgc tgtatcgcca ggcattgttg tcagatttct cgatgccgag
     6961 cgtacgcacc caatccgacg agcagaccgc cacgcagtgg atcaactggc tgggacgcta
     7021 cgcacaagaa gataaccgct ggttctcgtg ggtttctttc aatggtacta acattgacga
     7081 cagcaatcag caggcatttg cacggaaata tagccgggcg gcaggcaatg tcgatgacca
     7141 gatcaaccgc gtgctcaatg cactgcgtga ttctggcaaa ctggacaata cggtagtgat
     7201 tatcactgcc ggtcggggta ttccactgag cgaagaggaa gaaacctttg actggtccca
     7261 cggtcatctg caggtaccat tagtgattca ctggccaggc acgccggcgc agcgtattaa
     7321 tgctctgacg gatcataccg atctgatgac gacgctgatg caacgcctgc tacatgtcag
     7381 cacacctgcc agcgaatatt cgcaaggtca ggatttgttc aaccctcaac gccgtcatta
     7441 ctgggttacc gcagcggata acgatacgct ggcaattacc accccgaaaa agacgctggt
     7501 gctgaacaat aacggtaaat accgcactta caacttacgt ggtgaaagag tgaaagatga
     7561 aaaaccacag ttaagtttgt tattgcaagt gctgacagac gagaagcgtt ttatcgctaa
     7621 ctgattaatt ataaatcagt tagcgaaata tcttacttgc aatcggtgtg gaaaacggta
     7681 gtattagcag ccacgagtcg gcacgtagcg cagcctggta gcgcaccgtc atggggtgtc
     7741 gggggtcgga ggttcaaatc ctctcgtgcc gaccaaaaat cccaagaaaa aaccaaccct
     7801 tacggttggt ttttttatat ctgcaattaa ttcgataaac agaccgtgac acatcacagc
     7861 ctgtttattt tctgttatca gaacgtccag accacacccg cctgagtatt ccacggcgat
     7921 tctacccctg cgccattgcc atagctgact gacaaatgac cgcttaacgt cggggtaaac
     7981 gatgacctta tcccagcctg ataaacgcca ctggttccag ccacatcatt attaaatttg
     8041 ccatcgtcat taactttcac ctggttagaa tcggcgtatt cctgacgcac ggccgctttc
     8101 agccagggtt ccagcgtcgt accgttttgc aggtccatgt gatagcttac cgccgttccc
     8161 gcttcagcgc gtaatatccg ggtatttccc acatccgcgc gcatgccgtt tgataacgtg
     8221 tagtcctgac catctgtggt aaagccggta aaggccagat agggtctaac actccacaat
     8281 ccgtcaaccc aacggaaccc gctctcaaca tgagcacccg cgccgttact attgtaatcg
     8341 ccaaacgctg ttgccccatt actcatcttg ccatggatgg tgttggcaaa acggtcaact
     8401 ttcaccaccc catcaacata ggcaccgttc tgatgctccc aaccggcata agcccccagg
     8461 gtatagctat cgatattacc tttgccgccg cgatcaaaac caatatcaga atgagagtaa
     8521 ccaaagatca agccgcgaat tgtactgctt tcttcacggg agaaacggct atcgataccg
     8581 agcgtcaggc ccgtcaatgt ttgctcaaaa ccagctcccg catcagtggt cacgttgttg
     8641 cgggtgttaa ttgccgaact ccacatcgcc gtatcgtaac taacgccttt tacgctacca
     8701 agacgctcac gcacggtgtc cagttctgca tcaaatacca gcggttgtgc ggccgccata
     8761 ttcagcacat cagtggttga aggggtaatt tgcgcgcgat tctctgccag actccagcta
     8821 tggttgccat tatccagcaa ggtatattca tacgtaccga tatcaacaac gcctccggca
     8881 ttgcccaacg taaatgcagc atcaccgccg cccgttgtta ccagtgtaag gctatctcct
     8941 gctgccgggc tggcaccggt gtacgtcacg aatattttga aatcacctgt tgcctgaccg
     9001 gtgacgttga gctgatcgct ctgatgatta gccatatcgg tacgcatata aaaattgccg
     9061 tttcccgaca gggtattggt ggtcaacgta atgaaatttt ccgcacttgc cgtcactgat
     9121 gaacgagtca ctggctcatc catcataatc acatttgcgt cagacatcgc aaggttggct
     9181 aagttgagga gggtgttttg gttcgtcgta gtgatatcgg caatgatggt ccatacgcta
     9241 ttttcgagcg aaacgtccgc tttgctggta tccacggcac catgcgttct aaaagaagcg
     9301 ccctcagaaa ccactacctt acccaccaac attgcgggtg cataatttat ttgcggcttc
     9361 atgacctcaa gaatgccatc attccctctg actgaaacgt taaccattgt gccagcccag
     9421 acgttagcgc ggccattgtt gataaccata ttttcagaga tggcttttga ggaatacgtg
     9481 atacttccat tctgatatga tcgaccgaga tcatataccg catttgcctg cactttcgta
     9541 ccagtatcct ttcccagcga ttgcatagtg gcatgcttat caaggatagt atcaatggcc
     9601 tgcgtgtcct ccataacaat gagcccggaa ccatcatcca gtgcataatt ttttgacaca
     9661 ccatctttta tactaaattg gcctttactg tttggaccac tcacttccag cgcattcgtt
     9721 gagacaatta attttccgcc tgctttttta tcaacgccag ttacagtccc gccatccata
     9781 acctccagta agccgccact atctacagtg gtattataag cgaagtcatt ttcttcaact
     9841 cgcaaacttc cgccgttttc caacaacata ttattagcaa taccattctt gatatcgaac
     9901 tgaccgagac ggtttgttcc gaatacctcc atggcccgcg tggttgtttt aatagcaccg
     9961 cctgctttct gatctaccgc aaatgcaaat cccccctctt taacctccag tagtcctccc
    10021 ttttcgatag cgacaaagac ggccccccca tctttttcaa cgattaagcg cccgccttta
    10081 atcgtggtat cattcgctgc gccataaacc tgtagcacac cattattgag cgtagtctgg
    10141 tttgccgcgc cacccatttg aacaatttga taaccgtcat ttaatactga gtatt
//
LOCUS       DAPHBJ010000159        10144 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.161, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000159 DAPHBJ010000000
VERSION     DAPHBJ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.161"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            126..341
                     /gene="bdm"
                     /locus_tag="RHZ76_003492"
     CDS             126..341
                     /gene="bdm"
                     /locus_tag="RHZ76_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HDV2814079.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAQHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            443..580
                     /gene="sra"
                     /locus_tag="RHZ76_003493"
     CDS             443..580
                     /gene="sra"
                     /locus_tag="RHZ76_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HDV2814080.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            737..2434
                     /gene="maeA"
                     /locus_tag="RHZ76_003494"
     CDS             737..2434
                     /gene="maeA"
                     /locus_tag="RHZ76_003494"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDV2814081.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGMV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2568..3578
                     /gene="adhP"
                     /locus_tag="RHZ76_003495"
     CDS             2568..3578
                     /gene="adhP"
                     /locus_tag="RHZ76_003495"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HDV2814082.1"
                     /translation="MKAAVVTKDHHVDVTYKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSHTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3724..4008
                     /locus_tag="RHZ76_003496"
     CDS             3724..4008
                     /locus_tag="RHZ76_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDV2814083.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4415..5068)
                     /gene="fdnI"
                     /locus_tag="RHZ76_003497"
     CDS             complement(4415..5068)
                     /gene="fdnI"
                     /locus_tag="RHZ76_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HDV2814084.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(5061..5945)
                     /gene="fdnH"
                     /locus_tag="RHZ76_003498"
     CDS             complement(5061..5945)
                     /gene="fdnH"
                     /locus_tag="RHZ76_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HDV2814085.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5958..9005)
                     /gene="fdnG"
                     /locus_tag="RHZ76_003499"
     CDS             complement(5958..9005)
                     /gene="fdnG"
                     /locus_tag="RHZ76_003499"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8418..8420),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDV2814086.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATRPDQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            9237..10118
                     /gene="yddG"
                     /locus_tag="RHZ76_003500"
     CDS             9237..10118
                     /gene="yddG"
                     /locus_tag="RHZ76_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HDV2814087.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2463 a         2648 c         2569 g         2464 t
ORIGIN      
        1 atataaaaat catcaaccag gactaatctt aacaacgaag cggcaaatat ttgccatgct
       61 gaatgtgctt tataaatctg cgatccgtag cagacaccat aaatacacag acacggagaa
      121 tcactatgtt tacttattat caggcagaaa attcaacagc agaacccgct ctggttaatg
      181 ccattgagca aggccttcgg gcacagcacg gtgttgtcac tgaagatgac attctcatgg
      241 agctgaccaa atgggttgaa gcttctgata acgacatcct cagtgatatc taccagcaaa
      301 cgatcaatta tgtggtcagt ggccagcacc ctacgcttta aggtgctatg cttgatcggc
      361 aacctaattt aggggtttag cacgtgtttc ttcgctacgg cgatgttgtc cttaaaacta
      421 gctacaggat tgaggagtta aaatgaaatc gaaccgtcag gcacgtcata ttcttggact
      481 ggaccataaa atttctaacc agcgcaaaat agttaccgaa ggtgacaaat ccagcgtagt
      541 aaataaccca accggcagaa aacgccccgc tgaaaagtaa ttcataacca tcagtcctca
      601 atgacgatta aacaccattg cctgcgcaat ggtgtttttg tttttatctg ctttatactt
      661 gaggccgacg ccctggcggt aaagcaaaga cgataaaagc cccccaggga tggatattca
      721 aaaaagagtg agtgacatgg aaccaaaaac aaaaaaacag cgttcgcttt atatccctta
      781 cgctggccct gtactgctgg aatttccgtt gttgaataaa ggcagtgcct tcagcatgga
      841 agaacgccgt aacttcaacc tgctggggtt actgccggaa gtggtcgaaa ccatcgaaga
      901 acaagcggaa cgagcatgga tccagtatca gggattcaaa accgaaatcg acaaacacat
      961 ctacctgcgt aacatccagg acactaacga aaccctcttc taccgtctgg taaacaatca
     1021 tcttgatgag atgatgcctg ttatttatac cccaaccgtc ggcgcagcct gtgagcgttt
     1081 ttctgagatc taccgccgtt cacgcggcgt gtttatctct taccagaacc ggcacaatat
     1141 ggacgatatt ctgcaaaacg tgccgaacca taatattaaa gtgattgtgg tgactgacgg
     1201 tgaacgtatt ctggggcttg gtgaccaggg catcggcggg atgggcattc cgatcggtaa
     1261 actgtcgctc tataccgcct gtggcggcat cagcccggcg tatacccttc cggtggtgct
     1321 ggatgtcgga acgaacaacc aacagctgct taacgatccg ctgtatatgg gctggcgtaa
     1381 tccgcgtatc actgacgacg aatactatga attcgttgat gaatttatcc aggctgtgaa
     1441 acaacgctgg ccagacgtgc tgttgcagtt tgaagacttt gctcaaaaaa atgcgatgcc
     1501 gttacttaac cgctatcgca atgaaatttg ttcttttaac gatgacattc agggcactgc
     1561 ggcggtaaca gtcggcacac tgatcgcagc aagccgagcg gcaggtggtc agttaagcga
     1621 gaaaaaaatc gtcttccttg gtgcaggttc agcgggatgc ggcattgccg aaatgatcat
     1681 cgcccagacc cagcgcgaag gattaagcga ggaagcggcg cggcagaaag tctttatggt
     1741 cgatcgcttt ggcttgctga ctgacaagat gccgaacctg ctgcctttcc agaccaaact
     1801 ggtgcagaag cgcgaaaacc tcagtgactg ggataccgac agcgatgtgc tgtcactgct
     1861 ggatgtggtg cgcaatgtaa aaccagatat tctgattggc gtctcaggac agaccgggct
     1921 gtttacggaa gagatcatcc gtgagatgca taaacactgt ccgcgtccga tcgtgatgcc
     1981 gctgtctaac ccgacgtcac gcgtggaagc cacaccgcag gacattatcg cctggaccga
     2041 aggtaacgcg ctggtcgcca cgggcagccc gtttaatcca gtggtatgga aagataaaat
     2101 ctaccctatc gcccagtgta acaacgcctt tattttcccg ggcatcggcc tgggtgttat
     2161 tgcttccggc gcgtcacgta tcaccgatga gatgctgatg tcggcaagtg aaacgctggc
     2221 gcagtattca ccattggtgc tgaacggcga aggtatggta ctgccggaac tgaaagatat
     2281 tcagaaagtc tcccgcgcaa ttgcgtttgc ggttggcaaa atggcgcagc agcaaggcgt
     2341 ggcggtgaaa acctctgccg aagccctgca acaggccatt gacgataatt tctggcaagc
     2401 cgaataccgc gactaccgcc gtacctccat ctaagcctgc gcccggtagt gaaggctacc
     2461 gggctatttc cctctccctt tttcagatct catccatact gggtagtggc gaataaatct
     2521 catttgcctc acctgctatg cagaacatca tccgaaaagg aggaactatg aaggctgcag
     2581 ttgttacgaa ggatcatcat gttgacgtta cgtataaaac actgcgctca ctgaaacatg
     2641 gcgaagccct gctgaaaatg gagtgttgtg gtgtatgtca taccgatctt catgttaaga
     2701 atggcgattt tggtgacaaa accggcgtaa ttctgggcca tgaaggcatc ggtgtggtgg
     2761 cagaagtggg tccaggtgtc acctcattaa aaccaggcga tcgtgccagc gtggcgtggt
     2821 tctacgaagg atgcggtcat tgcgaatact gtaacagtgg taacgaaacg ctctgccgtt
     2881 cagttaaaaa tgccggatac agcgttgatg gcgggatggc ggaagagtgc atcgtggtcg
     2941 ccgattacgc ggtaaaagtg ccagatggtc tggactcggc ggcggccagc agcattacct
     3001 gtgcgggagt caccacctac aaagccgtta agctgtcaaa aattcgtcca gggcagtgga
     3061 ttgctatcta cggtcttggc ggtctgggta acctcgccct gcaatacgcg aagaatgtct
     3121 ttaacgccaa agtgatcgcc attgatgtca atgatgagca gttaaaactg gcaaccgaaa
     3181 tgggcgcaga tttagcgatt aactcacaca ccgaagacgc cgccaaaatt gtgcaggaga
     3241 aaactggtgg cgctcacgct gcggtggtaa cagcggtagc taaagctgcg tttaactcgg
     3301 cagttgatgc tgtccgtgca ggcggtcgtg ttgtggctgt cggtctaccg ccggagtcta
     3361 tgagcctgga tatcccacgt cttgtgctgg atggtattga agtggtcggt tcgctggtcg
     3421 gcacgcgcca ggatttaact gaagctttcc agtttgccgc cgaaggtaaa gtggtgccga
     3481 aagtcgccct gcgtccgtta gcggacatca acaccatctt tactgagatg gaagaaggca
     3541 aaatccgtgg ccgcatggtg attgatttcc gtcactaaga ggcctttgct gcgactgcca
     3601 tgttcgggtc gcagcatcgc acactctcca acatgaaatg gctaaatgga ttattgttgc
     3661 gtaggaagga acaataatga tagtgttccc agtgaaggaa cgctactgag agtaaagaca
     3721 ctaatgaaaa tggccaatca tccccgcccg ggggacatta ttcaggaatc actggacgaa
     3781 cttaatgtca gcctgcgcga gtttgccaga gcaatggaaa ttgctccctc aacggcaagc
     3841 cgattgctga caggaaaagc agctttgacg ccagaaatgg caattaaact ttccgtggtg
     3901 atcggcagtt cgccgcaaat gtggctgaat ctgcaaaacg cctggagtct ggcagaggca
     3961 gaaaaaacgg tggatgtgtc gcgattacgc cgtctggtta cgcaataaaa aaatggcact
     4021 ttataaagcg ccatttagaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat
     4081 caggcctaca cgttcactgc aatctattgg atttgaaggg gttgtaggcc ggataaggcg
     4141 ttcacgccgc atccggcatg aacaaagcct acgttgtcta caatatgaaa atgacgcctt
     4201 ttacagcgcc atttgaaact gatgacaaac gcaacattgc ctgatgcgct acgcttatca
     4261 ggcctacacg ttcactgcaa tctattggat ttgaagggtt ttcatgccgg ataaggcgtt
     4321 cacgccgcat ccggcatgaa caaagcctac gttgtctaca atatgaaaat ggcgcctttt
     4381 acagcgccat tttcagctta aagttcaaaa gagattatat cccttcttca ctctcttttt
     4441 tcgcttctgc cttctcgatt tcacgatacc agcgcggatg gtgtttcttc gcccagcgac
     4501 gacttacctt cccttcgatc atccctttaa tcgatccttt cacccaaaat gccatataca
     4561 tatggatcag gatggcgtgg atcaggatga tgccggcagc cgcgtggatc agcaggctgt
     4621 agcgaacaac ctgcatcggg aagtactgcg caaagtacgg acgccagata atcaccccgg
     4681 tcaccagcag cacgaaaatc atgctcatga tcgaccagaa catcatcttt tgcccggcgt
     4741 tgtacttacc aacatccgcc actttgtgct cattgccttt taatacttcg acaatgttca
     4801 acagccacgg aatatctttc ttatccggga tgttgtgatg cacaaaacgc acaaacataa
     4861 acatcagtgc gacgaaaatc gcaatgccga agaacgggtg caaaatgcgt cccatctgcg
     4921 gcgtaccgaa ggtttgcgtc agccattgca gcgtcgggaa gaagaacgaa atcccggaca
     4981 gcgccaccag gaagaagcaa atcaccacgg tccagtgaca ggcgcgatca ataaatttgg
     5041 tgcgcacaat cattttcgac ttactcatga tgatcctcct cgtcatcgtc cacttcctta
     5101 ttcgggccaa taccgatgta gtggaaaatc aacccggcaa aagtggcaat aaagccagcc
     5161 gctgccagcg gtttcaacgc gcctttccac aggcttaccg aggtgtcgat cttcggatct
     5221 ttcggcagac cgtgatacag ctccggctga tcggcgtgat gcagcacgta cataacgtgc
     5281 gtaccaccga ccccttccgg gttgtagacg ccagcatgtt cgtaaccacg cgctttcagt
     5341 ttcgccacgc gctgttccgc cagctccagc atctccttct tggtgccgaa gtggatagcc
     5401 ccggtcggac aggttttcac acaagccggt tcctggccga cgctgacgcg atcgacgcag
     5461 agcgtgcatt tatatacccg gttatcctct ttgttgaggc gcggaatatt aaacggacac
     5521 ccggcaatgc agtaaccaca gccgatgcag ttttccgact ggaaatcgac aatcccgtta
     5581 gcgtactgaa tgattgcacc agcagacggg cacgccttaa ggcagcccgg atcttcacag
     5641 tgcatacagc cgtctttacg gatcagccac tccagcttgc cgttctgttc ggtttcgcta
     5701 aagcgcatca ccgtccagga cttggcgctc agatcggcgg ggttatcgta aaccccgacg
     5761 cagtgcccca cttcatcacg gatgtcgttc cactccgaac acgccacctg acaggcttta
     5821 caaccgatac aggtggaaac gtcgataagt tttgcgactt ctgctttgta atcacgcacc
     5881 tgagaaggcg gcgtgatgga gttagttgcg gaccttttga taatgtcctg cgtttccata
     5941 gccatctgtt cgccccctta cgccttctcg atgttgacta agaacgcttt atattccggc
     6001 gtttgcgagt ttgcatcacc gacattcggc gtcagagtgt tagcgatata acctttacgc
     6061 gcgacaccct caaagcccca gtggattgga atacccaccg tttcaacctg ctgaccgttt
     6121 acattcagcg gcttcagacg gcgcgttacc actgccaccg cgcggataaa gccacgtttg
     6181 cttgagacag tgacacgatc gccattatta atgcctttcg ccgccgccag cgtttcgctg
     6241 atttccacaa actgttccgg ctgagcaatt gcgttgagca atgcgtgctt ggtccaggtg
     6301 tggaagtgct cggtcagacg ataggtcgta cccacatacg ggaactgctc ttttttaccc
     6361 atccgcagcg cgtcttgttc atacagacga acgaccgggt tagacaccac gttcggatgc
     6421 agcgggttag tgcccagcgg cgtttcaatc ggctcgtagt gttccgggaa cggaccttcc
     6481 gccattttgt tgatggcaaa caggcgtccc atcccttccg gctgcatgat aaacggcccg
     6541 gttggcgtac ccggtgcggc attgccgaag tcaggaatat cgttacccgt ccacttgctg
     6601 ccgttccact ggatcagcat ccgtttcgga tcccacggtt taccattgat atccgccgaa
     6661 gcacggttgt agagcacgcg acggttgagc ggccacgccc aggcccatcc cagcgtattc
     6721 cccagaccgg atgggtcgga gttatcgcgg ttagccatct ggttgccctg ctctgtccag
     6781 ctaccggtgt agatccagca agaagatgcg gttgtaccgt catcacgcag atgcgcaaag
     6841 ctactcagca actgaccttt cttcgcaatc agcacgccat tagcgtcata gagatcttcc
     6901 agcgcatagc cgttgttctc tttagccact tcgtcagatt gcggttcgtg cggttgcttg
     6961 tagttccagc tcatcttcat cagcggttct acgcctttac caccttcggc ctggtacagc
     7021 tcgcgcagat gatggtaaat acccgccaga atttcgccgt cgttacgcgc ttcgcccggc
     7081 gcgtcctgac ctttccagtg ccactgcaac cagcgaccgg agttagcgat agaaccatct
     7141 tcttcagcaa agcaggtcga agggagacgg aatacttcag tctgaataga cgccggatcg
     7201 acatcgttcg actcgccgtg gttctgccag aaggtagagg tttcagtcac cagcggatcg
     7261 ataaccacca tgtacttcag cttgctcagg cagctcacca ctttgttttt gtccgggaag
     7321 gacgcaaccg ggttaaagcc ctggcagaaa taaccggtga ctttgccttc gtccatcatg
     7381 ttgaaatact tgatgacgtc gtaggtctgg tcccacttcg gcagccagtc atagccccag
     7441 ttgttctctt tctgcgcggc atcgccatag aaagatttca tcaggctaac gaagaacttc
     7501 ggatagttgc tccagtagtt cacctggtca ggccgcgtcg ctttcggcgt gttcgcttcc
     7561 agatacgact gcaaatcaac ctgtttttct gacggcagcg tcagataacc tggcaggctg
     7621 gtagagagca ggcctaagtc agtcaggccc tgaatgttgg agtgaccacg caatgcgttc
     7681 acgccgccac cggccatacc catgttaccg agcagcaact ggatcatcgc catagtacgg
     7741 atgttctgcg cacccacggt gtgctgcgtc cagcccagcg cgtacaggaa ggtggttgtg
     7801 cgatccggtg cgctggtgga ggccagcact tcacacactt tcaggaagtc ggcttttggc
     7861 gtaccacaga tgttttcaac aacgtccggc gtgtagcggg aaacgtgcgc tttcagcagg
     7921 ttccacacac agcgcggatg agtcagtgtt tcatcgcgtt tcgcatagcc gttttcatcg
     7981 aactgatagt tccaggacga tttatcgtat tgccgttttt cagcgtcgta gccgctgaac
     8041 agaccgtctt cgaaagcaaa atcatcacgc accagcaagc tggcgttggt gtaatgctta
     8101 acgtattcgg cgttgatttt gttgttttcg atcaggtagc gcaaaacgcc agacaggaac
     8161 gtaatgtccg taccggaacg aataggtgcg taaatatccg ctacagaagc ggtacgcgta
     8221 aaacggggat cgacaacgat caaggttgca tcgttgttgt ttttcgcttc catcgcccag
     8281 cggaaaccga cgggatgcgc ttcagcagcg ttaccgccca ttaccattac gacgttagcg
     8341 tttttgatat ccacccagtg gttggtcatc gcaccgcgac caaatgttgg agcaagactt
     8401 gctaccgttg gtccgtgtca gacgcgcgcc tggttgtcta ccgccagcat cccgagggag
     8461 cgggcaaatt tctgggtcag catcccggtt tcgttgctgg caccggaagc acacagcata
     8521 ccggtagaaa gccaacggtt taccgttacg ccctgctcgt tcttttcaat aaagttagcg
     8581 tcacggtcag ctttcatcag cttcgcaata cgggagaatg cttcttccca gctaatgcgc
     8641 tgccatttgt cagaacctgg cgcacgatat tccgggtagc gcagacggtt ttcgctgttg
     8701 acgtaatcca gcaaaccggc ccctttcggg cacagcgcac cacggcttac cggatgatcc
     8761 ggatcacctt caatgtgata aatcgcttct ctggcgtttt tcgcgccatc acccaggcta
     8821 tacatcaata gcccgcaacc tacggaacag tatgtgcagg tgttacggat ctctttagcg
     8881 cgtaataatt tgtagtttcg cgcctgagcc agtgcttgct tcggggcaaa gcccaatgcc
     8941 gctactgttg ttccagccat accgcccgcg cagattttaa aaaattgtct gcgactgacg
     9001 tccattgctt tcctcttttt tcaggggtat tactgcgcga ggaaactaac agtaaaattc
     9061 tgaatctttt tgcgcgaaat caaaaagaac acgtgtttgc cacaaaataa tccgcctgcg
     9121 caaattgacc tacctcaata gcggtagaaa aacgcaccac tgcctgacag gccagttaaa
     9181 aaaatgctat aaaattcagc ttaattttta acggcaagag agacaaaaca gcgagcatga
     9241 cacgacaaaa agcaacgctc atagggctaa tagcgatcgt cctgtggagc acgatggtag
     9301 gattgattcg cggtgtcagt gaggggctcg gcccggtcgg cggcgcagct gctatctatt
     9361 cattaagcgg gctgctgtta atcttcacgg ttggatttcc gcgtattcgg caaatcccga
     9421 aaggctattt actcgccggg agtctgttat tcgtcagcta tgaaatctgt ctggcgcttt
     9481 ccttagggta tgcggcgacc cgtcatcagg cgattgaagt gggtatggtg aactatctgt
     9541 ggcccagcct gacaattctc tttgccattc tgtttaatgg tcagaaaacc aactggctga
     9601 ttgtacctgg attattatta gccctcgtcg gcgtctgctg ggtgttaggc ggtgacaatg
     9661 ggttacatta tgatgaaatc atcaataata tcaccaccag cccattgagt tatttcctgg
     9721 cgttcattgg tgcgtttatc tgggcagcct actgcacagt aacgaataaa tacgcccgcg
     9781 gatttaatgg aattaccgtt tttgtcctgc taacgggagc aagtctgtgg gtttactatt
     9841 ttctcacgcc acaaccagaa atggtattta gcacacccgt catgattaaa ctcatctctg
     9901 cggcatttac cttaggattt gcttatgctg catggaatgt cggtatattg catggtaacg
     9961 tcaccataat ggcggtaggt tcgtatttta cgcccgtact ttcctcagcg cttgcagccg
    10021 tgctgctcag cgccccgctg tcgttctcgt tctggcaagg cgcgctaatg gtctgcggcg
    10081 gttccctgct ctgctggctg gcgacacgtc gtggttaaaa taattgccgg gtctacggcc
    10141 tggc
//
LOCUS       DAPHBJ010000160        10090 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.162, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000160 DAPHBJ010000000
VERSION     DAPHBJ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.162"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            252..2924
                     /gene="rcsD"
                     /locus_tag="RHZ76_003501"
     CDS             252..2924
                     /gene="rcsD"
                     /locus_tag="RHZ76_003501"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HDV2814088.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDNWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVNDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTENVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            2941..3591
                     /gene="rcsB"
                     /locus_tag="RHZ76_003502"
     CDS             2941..3591
                     /gene="rcsB"
                     /locus_tag="RHZ76_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HDV2814089.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(3791..6640)
                     /gene="rcsC"
                     /locus_tag="RHZ76_003503"
     CDS             complement(3791..6640)
                     /gene="rcsC"
                     /locus_tag="RHZ76_003503"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HDV2814090.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMLVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLSVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            6807..8632
                     /gene="atoS"
                     /locus_tag="RHZ76_003504"
                     /pseudo
     CDS             6807..8632
                     /gene="atoS"
                     /locus_tag="RHZ76_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
     gene            8629..10014
                     /gene="atoC"
                     /locus_tag="RHZ76_003505"
     CDS             8629..10014
                     /gene="atoC"
                     /locus_tag="RHZ76_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HDV2814091.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
BASE COUNT         2693 a         2571 c         2387 g         2439 t
ORIGIN      
        1 aactatttca caaataatta acatcagcat aatttccagc aatcttcgtt tatttgcaat
       61 taatttcgtt gggctttttg taggttattt gtacagcaaa atggcgcttg tacatctatt
      121 tcccccaatg caggatgata aatatcacgg gagaatagag aatcatcaat caggtaagag
      181 tctggaattt cacactgtac cctttatact gccctatcac ttcgcgaagt tttaacaggt
      241 cataaacacg aatgcgtcag aaagagacaa cggccacgac ccgcttttca ctcctaccgg
      301 ggagcattac ccgcttcttt ttactgttga tcattgtgtt actggtgacg atgggtgtaa
      361 tggtacaaag cgccgttaac gcctggctga aagataaaag ttaccagatt gtcgacatta
      421 cccatgctat ccaaaagcgc gtcgataact ggcgttacgt gacctggcag atctacgaca
      481 acattgccgc gacgacctcc ccctcctccg gcgaaggttt acaagagacg cgcctgaaac
      541 aggatgtcta ctatctggag aaaccacgcc gcaaaacgga agcgttaatc tttggctctc
      601 acgacaactc aacgcttgag atgactcagc ggatgtccac ttatctggat acattgtggg
      661 gcgcagaaaa tgtaccgtgg tcgatgtatt acctgaatgg tcaggataac agtctggtgc
      721 tgatctcaac cctacccctt aaagatctca cctccggatt taaagaatcg accgtcaacg
      781 acattgttga ttcacgtcgt gcagagatgt tgcaacaggc caacgccctc gatgaacgcg
      841 aaagcttttc taacatgcgc cgcctggcct ggcagaacgg tcattacttt acattgcgta
      901 ctacctttaa ccagccagga catctggcaa cggtcgtggc ttttgatctg ccgattaatg
      961 atttgatccc accgggtatg ccgctggaca gtttccgcct tgagccagac gcgacggcaa
     1021 cgggaaacaa tgataatgag aaagaaggga cggatagcgt cagtatccac tttaacagta
     1081 cgaagattga aatctcctcg gcactcaact ctaccgatat gcgcctggtc tggcaggttc
     1141 cttatggcac cttattgctg gatacgttgc aaaacattct gctgccactg ctgctgaaca
     1201 tcggtttgct ggcgctggcg ttatttggct ataccacatt ccgccatttc tccagccgca
     1261 gtacagaaaa cgtccccagc acggcggtca ataacgaatt gcgcatttta cgggcaatca
     1321 atgaagagat agtctcactg ctgccgctcg gcctgctggt tcacgatcag gaatcgaacc
     1381 gcactgtcat aagtaacaaa attgccgatc atttgctgcc gcatttgaat ctgcaaaaca
     1441 tcaccaccat ggcggaacag catcagggga ttattcaggc gacgatcaat aacgagctgt
     1501 atgagatccg catgttccgc agccaggtcg cgccgcgcac acaaattttc attattcgcg
     1561 atcaggatcg cgaagtgctg gtaaacaaga aactcaagca ggcgcagcgt ctgtatgaga
     1621 aaaaccagca ggggcggatg acctttatga aaaacattgg cgatgcgctg aaagaacccg
     1681 cacagtccct ggcggagagc gcggctaaac tcaatgcccc ggaaagcaaa caactggcga
     1741 atcaggcaga tgtgctggtg cggctggtcg atgaaataca gttagcgaac atgcttgcgg
     1801 acgatagctg gaaaagtgag acggtgctgt tctccgtgca ggatttaatt gatgaagttg
     1861 tcccttcagt gttgcctgcc atcaagcgta aaggtctgca actgctgatt aacaatcatc
     1921 tgaaagcaca cgatatgcgc cgcggcgatc gcgatgcctt acgacgtatt ttgctgctac
     1981 tgatgcaata tgccgtgacc tcaacgcaat tgggaaaaat cacccttgag gttgatcagg
     2041 atgagtcctc cgaagaccgc ctgacgttcc gcattctgga caccggagaa ggcgtaagca
     2101 ttcatgaaat ggataatttg cacttcccgt ttatcaacca gacccaaaac gatcgctatg
     2161 gcaaggcgga cccgctggca ttctggctga gcgatcaact ggcacgtaaa ctgggcggtc
     2221 atttaaacat caaaacgcgg gatgggcttg gtacacgcta ctctgtgcat atcaaaatgc
     2281 tcgcagctga cccggaagtt gaagaggaag aagagcgttt actggatgat gtctgcgtaa
     2341 tggtggatgt tacttcggca gaaattcgga atattgtcac tcgccagtta gaaaattggg
     2401 gtgcaacctg tatcacaccc gatgaaagat taattagtca agattatgat atctttttaa
     2461 cggataatcc gtctaatctt actgcctctg gcttgctttt aagcgatgat gagtctggcg
     2521 tacgggaaat tgggcctggt caattgtgcg tcaacttcaa tatgagcaac gctatgcagg
     2581 aagcggtctt acaattaatt gaagtgcaac tggcgcagga agaggtgaca gaatcgcctc
     2641 tgggcggaga tgaaaatgcg caactccatg ccagcggcta ttatgcgctc tttgtagaca
     2701 cagtaccgga tgatgttaag aggctgtata ctgaagcagc aaccagtgac tttgctgcgt
     2761 tagcccaaac ggctcatcgt cttaaaggcg tatttgccat gctaaatctg gtacccggca
     2821 agcagttatg tgaaacgctg gaacatctga ttcgtgagaa ggatgttcca ggaatagaaa
     2881 aatacatcag cgacattgac agttatgtca agagcttgct gtagcaaggt agcctattac
     2941 atgaacaata tgaacgtaat tattgccgat gaccatccga tagtcttgtt cggtattcgc
     3001 aaatcacttg agcaaattga gtgggtgaat gttgtcggcg aatttgaaga ctctacagca
     3061 ctgatcaaca acctgccgaa actggatgcg catgtgttga ttaccgatct ctccatgcct
     3121 ggcgataagt acggcgatgg cattacctta atcaagtaca tcaagcgcca tttcccaagc
     3181 ctgtcgatca ttgttctgac catgaacaac aacccggcga ttcttagtgc ggtattggat
     3241 ctggatatcg aagggatcgt gctgaaacaa ggtgcaccga ccgatctgcc gaaagctctc
     3301 gccgcgctgc agaaagggaa gaaatttacc ccggaaagcg tttctcgcct gttggaaaaa
     3361 atcagtgctg gtggttacgg tgacaagcgt ctctcgccaa aagagagtga agttctgcgc
     3421 ctgtttgcgg aaggcttcct ggtgaccgag atcgctaaaa agctgaaccg cagtattaaa
     3481 accatcagta gccagaagaa atctgcgatg atgaagctgg gtgtcgagaa cgatatcgcc
     3541 ctgctgaatt atctctcttc tgtgacctta agtccggcag ataaagacta atcatctgta
     3601 ggccagataa gacgcgttag tgtcttatct ggcatttgca ccgattgccg gatgcggcgt
     3661 aaacgcctta tccggcctac gattcccatt atttcaacag attacattaa agtaggccag
     3721 ataagacgcg tcagtgtctt atctggcatt tgcactgaat gccggatgcg gcgtaaacgc
     3781 cttatccgtc ctacgaatcc cgcgatttcc tgaccctctc ggcatatacc gtcagcgtct
     3841 gttttatcac atccagcgtt accggcttcg acaggcagct gtccataccg gactccagac
     3901 accgctgctt ctcttcagcc aacgcattag cagttactcc gattaccggc aacgtcagtc
     3961 ccaactgacg aatgcgttgc gtcaagcggt aaccatccat atttggcatg ttgacgtcgc
     4021 taagcacgat atcaatatga ttcttgctaa gtacattaag cgcatcgaca ccatcattcg
     4081 cggttttaca ttgatagccc aacgatccca actgatctgc cagcagacgt cggttaatcg
     4141 gatgatcatc cacgaccaga atcatcatat cgtcattatc gctgaccgct ttgtccgtcg
     4201 acggcagagc gttagcagga tcgtcgctct ccatctcgat caaataaata cgcgccaaca
     4261 atgccggtag ctcatgcggg gcagccacac tgtgtaccca ctcccctggc gctttctcca
     4321 gcggaatacc aatatggcga cgacagaagg tcactaccgc tctaccctgc cattttttac
     4381 tcactacctc gtcagtaatt aacacatctt cgggagtcgg ttcctgccct tcgtatgttg
     4441 taacgacgat gccgctgcgc tgcaaactgg tttccaggaa ctgacagagc gacgcattgc
     4501 ggaccgacag ccagcagcgt ttaccactca acccttccac gccttttttc tgcgggtact
     4561 gagcgccgta caacggaata cgcacggtaa actggctgcc cattcccggt tctgaatcta
     4621 ccgagatatc gccgtccatc atgctgatca gtttttcaca aatcgccaga cccagaccgg
     4681 tcccctggaa gtttcgctgt acgcccgttc cgacctggaa gaagggatca aacaagcgca
     4741 ccacttcttt cgccggaatc cccacgccgg tatcgcgaac acggatagag agataatcgc
     4801 catcagcgcg gacatgcaaa actatacagc cggtatcggt gaattttatg gcgttactca
     4861 acaggttgga gatgacctgc tgtaaacgca tcgggtcgcc atttaaggcc actggcacat
     4921 ccggttcaat aaagcagtac aagcctaact gcttgcgtac caccagcggt aaatagttgg
     4981 cggtgatgtg gttcatcact tcacgcggtg aaaactcacg cggttcgatc ttcaactgtt
     5041 ccgattcaat cttcgagaaa tcgagaatat cgctgataat tttcaacaac aggctggaag
     5101 agttgttcat tgccgtcacc agccgatcga cgcctttcgg taactctttg gtttgcaaca
     5161 gatccaggtt accgataatg ccatacagcg gcgttcgcag ctcatgactg acggtggcaa
     5221 ggaacatcga ttttgactgg ctcgcctgtt ccgctgcttg tgccatctcc tgcaacgact
     5281 cttccatctt cacgcgcgaa gaaacatcca ccagcacaca aatggccacg ttttcattac
     5341 gatagcgcga atggacgaag ctgatttgca gattggtatt gttgctggtc aggacatcaa
     5401 caaaattgac ctgctgccca cagataattt gcgtcagtcg ttggcggtcc tcatgcgtaa
     5461 gcatattgag ataggtatgc gccagttcgt tacttaaaat attgacgcca tcagcggtac
     5521 gcaaaatgca gatacccact ggcgcggagg cgacaatctt gcgattgaac tgctcatgtt
     5581 cttccagtcg cagggcgtcg ctttccgccg gaatgaaaat acgtcgctcg tacatccgtg
     5641 cgagagtaaa caatgcagct ccggcaagca cattcagcaa aattgcgtta aggatcaaca
     5701 tgcgaatgcg ttccagcacc ttatcaaccg gcaccgaata cacgatgctt agcgatgagg
     5761 gtggcagatt tttcttcagc accagctccc ggaacccttc cgtatagcca aaccaggagc
     5821 gttcctgcat ccagcgagga tcgcccttaa ttttactttc tggtccggta agcgaaatca
     5881 gggtatgacc attttcatca agaatggtaa cccccatcgg caacgtaccc ggtaagaaaa
     5941 agttctccat ccggatggtc tgctcgacac ccaaaagcgc ctgcaaccgg ttcgccagat
     6001 aaactggcgt caacgcgtaa aaatacccga cgccagggcg cggaccttcg ctgatccagt
     6061 agaggttact gccgctatca tcttgtggtg catttcgata tttattgatg cgttcatgca
     6121 aagctttcaa cgcggtatcg cgttccacta gcatatcacg cagaccgaaa ttggccatgc
     6181 agaggttatc gctgccgatt aaaaataccc ggtttagatc gtaagccgca gaaaaattat
     6241 cgcgccagta gcgcataaac cacgccaatg actccagaga acctcgccag gtgttactca
     6301 ttgcggaaca atcggagtcg gcaaacagcg gttcaaacgc aggcacatcc gcctgcgttt
     6361 ctcgtccacg cggggaaagc acaccgtttt ctgccgataa gcgattttcg gcgatgtact
     6421 tcagctcttt catcacatca gaggtgcgtt gaataaagcg ctgagcctga tcggaactca
     6481 gattaaattc ctgacgaatt tccgattctc gctgatgtaa cgcattaacg atgtaaaaaa
     6541 cggatgaaaa agcaatcaac agccagagca ctaacgccaa tgctctgaac atgtagcgcg
     6601 aggctttcag ggttgtacga aaagaagcaa ggtatttcaa aggggcgaag ctccgcctca
     6661 ggtgaccgat ggagtgtggt taaggtagcg gtaaaagcgt gttaccgcaa tgttctctct
     6721 tctctggaat atgatacacc gccgagaaat catcacctta acctctgata atcgtcatat
     6781 accggacaag actagtggat ttcagcatgc attatatgaa gtggatttat ccacgccgct
     6841 tacgcaatca aatgatcctg atggcaatcc tgatggtcat tgtcccaacg cttactattg
     6901 gttatatcgt agaaacggaa ggacgttcag cagtctatct gaaaaagaga aaaaactttc
     6961 tgccgtggtc aacctgctta atcaggcact aggcgatcgc tatgatctct acatcgactt
     7021 accacgtgag gagcgtatcc gcgcattaaa tgcagaactt gcccccatta ccgaaaatat
     7081 cactcacgcc ttccctggca tcggtgctgg ttattacaac aaaatgctgg atgcgataat
     7141 cacctacgcg ccttcagcgc tatatcagaa taatgtcggc gttaccattg ccgcagatca
     7201 ccctggtcgc gaagtcatgc gtacaaatac ccctttggtt tattcaggca ggcaggtgcg
     7261 cggcgatatt ttgaattcaa tgctccccat tgagcgtaat ggtgaaatcc tcggctatat
     7321 ctgggccaat gaattaaccg aagatattcg ccgccaggcc tggaaaatgg atgtgaggat
     7381 tatcattgtg ctcaccgccg gtttgctgat aagcctgcta ttgattgtcc ttttctcccg
     7441 tcgcctgagc gccaatattg atatcatcac cgatggcctc tcgactctgg cacaaaatat
     7501 tcccactcga ttaccacaat tgcccggtga aatggggcaa atcagtcaga gtgttaataa
     7561 cctcgcccag gcactgcgtg aaacgcggac acttaacgat ctgattattg aaaacgctgc
     7621 cgatggcgtc attgccattg accgccaggg tgatgtaacc accatgaacc cagcagcaga
     7681 agttatcact ggctatcaac gccatgaact ggtagggcag ccttactcca tgttgttcga
     7741 caatactcag ttctacagtc cagtactgga tacgctggaa catggcaccg aacatgtggc
     7801 gctggagatc agttttccag gtcgtgaccg caccattgaa ctcagtgtca ctaccagtcg
     7861 tattcataac acgcacggtg aaatgatagg tgctttggtg attttctctg atttaactgc
     7921 ccgcaaagaa acccagcgcc gcatggcgca agcagaacgc ctcgccacac tgggtgagct
     7981 gatggctggc gtcgcgcatg aagtacgtaa tccgttaacg gctattcgtg gttatgtaca
     8041 gatcttgcgc caacaaacca gtgacccaat acatcaggaa tatctgtccg tagtactcaa
     8101 agaaatcgat tcaattaaca aagttattca gcaattgctc gaattttcac gtccacgcca
     8161 cagtcaatgg caacaagtca gcctcaatgc attggttgaa gaaactctgg tactggtaca
     8221 aaccgccggc gtacaagcgc gggtcgactt cataagcgaa ctggataatg aattaagccc
     8281 gattaacgcc gatcgtgaac tgctcaaaca ggtactactg aatatcctga tcaatgccgt
     8341 ccaggctatc agcgcacgag ggaaaattcg cattcaaacc tggcaataca gcgactcaca
     8401 acaggccatt tcgatagagg acaacggctg tggcattgat ctctcgctgc aaaaaaagat
     8461 cttcgatccc tttttcacca ccaaagcctc aggaaccggg cttggtctgg cgttaagtca
     8521 acgcatcatt aatgcccatc agggtgatat tcgcgtcgcc agtttgccgg gctacggcgc
     8581 aaccttcacg cttattttac cgatcaaccc gcagggaaat cagactgtat gactgctatt
     8641 aatcgcatcc ttattgtgga tgatgaagat aatgttcgcc gtatgctgag caccgctttt
     8701 gcactacaag gattcgaaac acattgtgcg aacaacggac gcacagcatt acacctgttt
     8761 gccgatattc accctgatgt ggtgttgatg gatatccgca tgccagagat ggacggcatc
     8821 aaggcactaa aggagatgcg cagccatgag acccggacac ccgttattct gatgacggcc
     8881 tatgcggaag tggaaaccgc cgtcgaagcg ctacgctgcg gagccttcga ctatgttatt
     8941 aaaccgtttg atctcgatga gttgaattta atcgttcagc gcgctttaca actccagtca
     9001 atgaaaaaag agatccgtca tctgcaccag gcactgagca ccagctggca atgggggcac
     9061 attctcacca acagcccggc gatgatggac atctgcaaag acaccgccaa aattgccctt
     9121 tctcaggcca gcgtcttgat tagcggtgaa agcggcaccg ggaaagagtt gattgccaga
     9181 gcgattcact acaattcgcg gcgggcaaag gggccgttca ttaaagtcaa ctgcgcggcg
     9241 ctgccggaat cgttgctcga aagtgaactg tttggtcatg aaaaaggtgc atttactggt
     9301 gcacaaacct tgcgtcaggg attatttgaa cgagccaacg aaggtactct gctcctcgac
     9361 gaaattggcg aaatgccgct ggtactacaa gccaaattac tacgcattct acaggaacgg
     9421 gaatttgaac ggattggcgg ccatcagacc ataaaagttg atatccgcat cattgctgcc
     9481 accaaccgcg acttgcaggc aatggtaaaa gaaggcacct tccgtgaaga tctcttttat
     9541 cgccttaacg ttattcattt aatactgccg cctctgcgcg atcgccggga agatatttcc
     9601 ctgttagcta atcacttttt gcaaaaattc agtagtgaga atcagcgcga tattatcgac
     9661 atcgatccga tggcaatgtc actgcttacc gcctggtcat ggccgggaaa tattcgagag
     9721 ctttccaacg ttattgaacg cgccgtcgtg atgaattcag gcccgatcat tttttctgag
     9781 gatcttccgc cacagattcg tcagccagtc tgtaatgctg gcgaggtaaa aacagcccct
     9841 gtcggtgagc gtaatttaaa agaggaaatt aaacgcgtcg aaaaacgcat cattatggaa
     9901 gtgctggaac aacaagaagg aaaccgaacc cgcactgctt taatgctggg catcagtcgc
     9961 cgtgcattga tgtataaact ccaggaatac ggtatcgatc cggcggatgt ataacaccaa
    10021 aacttgctat gcagaaattt gcacagtgcg caattttctg catagccgct cattctcctt
    10081 ataaatcccc
//
LOCUS       DAPHBJ010000161        10076 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.163, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000161 DAPHBJ010000000
VERSION     DAPHBJ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.163"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(335..622)
                     /locus_tag="RHZ76_003506"
                     /pseudo
     CDS             complement(335..622)
                     /locus_tag="RHZ76_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689479.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1233..1589)
                     /locus_tag="RHZ76_003507"
     CDS             complement(1233..1589)
                     /locus_tag="RHZ76_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDV2814092.1"
                     /translation="MSAPATILDMCCGSRMFWFDKSDERAIFSDIRKEGYTLRNGRRL
                     IISPDIIADFRALSFVDASFSMVVLDPPHLESVGDNAWMGKKYGRLNKDAWRDDSRQR
                     FKEAFRVLRPHGVLIF"
     gene            complement(1586..2008)
                     /locus_tag="RHZ76_003508"
     CDS             complement(1586..2008)
                     /locus_tag="RHZ76_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HDV2814093.1"
                     /translation="MAKVFTQEEREKIKWQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSSPDG
                     EIRRYDSRLNIICRECRKSEVMQRILAFYQGNFQDVAQ"
     gene            complement(2049..3014)
                     /locus_tag="RHZ76_003509"
     CDS             complement(2049..3014)
                     /locus_tag="RHZ76_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814094.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDGGKLSESIGSKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDPQTTEQDFLTRNSDAVVFSAKKRQWGSREDLACAQWIWGR
                     IVGLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            complement(2995..3516)
                     /locus_tag="RHZ76_003510"
     CDS             complement(2995..3516)
                     /locus_tag="RHZ76_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814095.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTYEGGAIDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REITDGKSITCMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            complement(3500..3730)
                     /gene="dicC"
                     /locus_tag="RHZ76_003511"
     CDS             complement(3500..3730)
                     /gene="dicC"
                     /locus_tag="RHZ76_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicB transcriptional regulator DicC"
                     /protein_id="HDV2814096.1"
                     /translation="MLKTDALLYFGSKTKLAQAAGIRLASLYSWKGDLVPEGRAMRLQ
                     EASGGELQYDPKVYDEYRKTKRAGRLNNENHS"
     gene            3814..4221
                     /gene="dicA"
                     /locus_tag="RHZ76_003512"
     CDS             3814..4221
                     /gene="dicA"
                     /locus_tag="RHZ76_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional dual regulator DicA"
                     /protein_id="HDV2814097.1"
                     /translation="METKNLTIGERIRYRRKNLKHTQRSLAKALKISHVSVSQWERGD
                     SEPTGKNLFALSKVLQCSPTWILFGDEDKQPTPPVEKPVALSPKELELLELFNALPES
                     EQDTQLAEMRARVKNFNKLFEELLKARQRTNKR"
     gene            4388..4543
                     /locus_tag="RHZ76_003513"
     CDS             4388..4543
                     /locus_tag="RHZ76_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HDV2814098.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            4703..4921
                     /locus_tag="RHZ76_003514"
     CDS             4703..4921
                     /locus_tag="RHZ76_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814099.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMRQKINAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            5489..5677
                     /gene="dicB"
                     /locus_tag="RHZ76_003515"
                     /gene_synonym="ftsT"
     CDS             5489..5677
                     /gene="dicB"
                     /locus_tag="RHZ76_003515"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HDV2814100.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            5674..5865
                     /gene="ydfD"
                     /locus_tag="RHZ76_003516"
     CDS             5674..5865
                     /gene="ydfD"
                     /locus_tag="RHZ76_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HDV2814101.1"
                     /translation="MNSAFVLVLTVFLVSGEPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            5958..8429
                     /locus_tag="RHZ76_003517"
     CDS             5958..8429
                     /locus_tag="RHZ76_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HDV2814102.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPSDVDAGNIDGSIHRWA
                     KKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     PEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGLQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKVCTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHNSDNETGETADHP
                     IKVNGHHEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSVIWQKLIPSQADS"
     gene            8502..8753
                     /locus_tag="RHZ76_003518"
     CDS             8502..8753
                     /locus_tag="RHZ76_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HDV2814103.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            8788..10068
                     /locus_tag="RHZ76_003519"
     CDS             8788..10068
                     /locus_tag="RHZ76_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV2814104.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKDVRVRENLGVPDTAKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPTMPHNEAMKN"
BASE COUNT         2886 a         2382 c         2234 g         2574 t
ORIGIN      
        1 taccttgctg tgaagatgat gggcatacat gatgcgaaca accagaacgc aacaaacaaa
       61 aactgcaatg cgtttttcat tattcctcct acaatcaatg tgcaattaca tttaaacaca
      121 cctcaatttg gccggacata taaatatcta aaccagaaaa aatcacttac atagcgttac
      181 aaactcttta gtctaaaggt tcatcgtaaa acattcccca tacttatcag tccgttccgc
      241 gccaggtagc tcattgcctt atctggcaat ctgtaatcag gtttccgctt tttcagttgg
      301 ctggtcgttt aaccgacata gttaatccat taatctagtt gccggatgtt ggtggatttt
      361 cgcgttttag ttgttcataa aagtgcacag ctttaaccag ttcttctgat gtaactggga
      421 ctggtgaggc agtgaataag gcctgaattt gatagttcgg cctgtcgtta caatcctctt
      481 ttttcggtac atatttccag tcaccagacc actacttccc ctgaaagtcc gtaacgcctt
      541 ttttcacgta gcgatatcgc catgccactg gttttgcttg ccccgccgtt tcatgccctt
      601 cctgataatt aatctcgctc attcatcgcc ccactcatca caatatgctt cgaccggagt
      661 ttttcccgct tcatagtcat cacgctagga ttcgacatca gcagcacttc caccgcgtaa
      721 ctcaccatag tccattaata gttcatcccg ctcttcaaaa ctgatgttat atttagctga
      781 acaaaaatca gccactttgt tcttcctcat cgtcttttat ttcgtggtat gagtaattgc
      841 agtagttaaa gaaaatttct tatgctccgt catgaatttc ctcaggtgtt gcgtcatcgt
      901 ccacttcgaa tacatcctca aaatctccac cagctattcc cgtttcaata ataattttga
      961 actttcgcat ttcactaccg ccctttcggg cggcctcctg atgttctgag ggtgcagaaa
     1021 tccatccggt taaggattaa attttattta cagaactgaa tttaattatt cagatatacg
     1081 tatctgtagc cttacgaatc tactcactgg atgcctcttt cataaaaata atccagtggg
     1141 ttttatcgtt ttttccctgt tcgttgacag ataacaggtt ttctgtctgt cagtgccaga
     1201 atctggctta ccggtatttg cgtttcattc cattaaaaaa tcagaacgcc gtgcggcctc
     1261 aacacccgaa aggcttcttt aaatctctgt cgcgaatcat cacgccaggc atctttattc
     1321 agccgtccat atttctttcc catccaggcg ttatcaccaa cactctcaag atgcggaggg
     1381 tctaatacaa ccatcgaaaa agatgcgtct acaaatgata atgcacgaaa atctgcgata
     1441 atgtcagggc tgataatcaa gcgtctccca ttgcgtaatg tgtatccttc ttttctgata
     1501 tcgctgaata tcgcccgttc gtcagactta tcgaaccaga acatgcggct gccacagcac
     1561 atatcaagaa tggttgccgg tgcactcact gcgccacgtc ctgaaaatta ccctgataga
     1621 aagccagtat gcgctgcata acttcgctct tccggcactc gcgacagatt atgtttaggc
     1681 gactgtcgta gcgacgtatt tctccgtcag gtgatgacca gataaggtcc ggatcaacca
     1741 cagcaggttt cttcaccttt gccctcgaga gttttttgcg ggcgttttgc cagtccttac
     1801 gcgcctgttc agaggggaat aacccgtagc cggagttgta tacatcgcca ctggctacca
     1861 gctctctggc aagaacgctc atcagatatc tagtcgcacc tgttttagct tccagttgcc
     1921 gtaacgtctc acggccgctc tggcgcacga gttccaccac ctgccattta attttttccc
     1981 gctcttcttg tgtaaaaact tttgccacaa gtccccctta aaattacctc atgacctgaa
     2041 atcaacactt atcctctgaa accaggcgga atttctgtat ccggttcaga aatatgatta
     2101 acacaacgct gtacaggtga acgtcccagg cggatgacca gttcgtccca tttttcgcgg
     2161 agctttgacg ggctcatgat gttttttacc cagaatggat ctcgctgaac ccgaccaaac
     2221 atttcgcaaa tttgtctgtg gcttctgcca tccagcatcc gcattgtgcg cacgtcattg
     2281 gcccaggcag tccagttagg ctcttttggt cgcatgatct cgccatcatc actggctgcc
     2341 tgttcgtaga gacccacgat ccgcccccag atccactgcg cacacgccaa atcctccctg
     2401 ctaccccact ggcgtttttt cgcactaaac acaaccgcgt cggagttccg ggttaaaaaa
     2461 tcctgttcag tcgtctgcgg gtccggttgc gaagcttccg gacgaaaagt gtttttattc
     2521 tctgtagtaa tctctgttgt attctctgta agatcatcag gccattttga cccgatgaca
     2581 ttgggtcgtt ttgaaccaat ggagcgtttc attttgacct cttccatcgt gtcattttga
     2641 cctgatggag cggcgcattt tgaaccgatg gattcgctca atttgccacc atctaaaagc
     2701 tcgctcccat agttgatcgt gtagaaattg gtcatatcgc gctttgattt attgagcttt
     2761 tcacaacgca aaagccccag cgttttcaga cttgcaaacg cgcgctttaa cgttgactct
     2821 gaccagaatg ggaactgttc cagccattgt tccgttgtgt tataaatcca gcgaacacca
     2881 tcacattcca tgccggagtt ggtatctctc aaccagtagt gcagttgttg caaaacaatg
     2941 gcttcgttta agccaatttt cattgccagc tgcgtgttta taaccagtgg gcgttcagca
     3001 aaaagaaggc tcataattcc atccagcttt ttgttggtat tgctgtcgat acgcaagttt
     3061 gaaagcaatt gctttttcta taagttcgtc agtttcacga tccactacgg caggatcagc
     3121 aaaaagcagt ccggactcca ccacatcgcc atattctttg tttaacccgg cgatcatgca
     3181 cgtgatgctt tttccgtcag taatttcacg atacaacctg aaatcattaa tccggatagc
     3241 ctccataatt gcaggcacta gcgccgtgaa cttttcacgc ttatccctgg tgtcgatagc
     3301 cttccagcgt tcaaatatct tcactcgatt aacgccaagc gctcgctgat caatcgcgcc
     3361 accttcatat gtgacacgct gaacatcgat gttcgggcgc tctttcaaag cccagaatgc
     3421 ttcagtgatt aatatcgtcg cctgctcctg tgtcattcct ggtcgacata tccaggcatc
     3481 cagagcctca cgagcctgtt caggagtgat tttcattgtt caaccgcccc gcccgcttcg
     3541 tcttacgata ttcatcataa actttgggat catactgaag ctccccgcca gatgcctcct
     3601 gtagacgcat cgcgcgacct tcgggaacta aatccccttt ccagctataa agcgaagcca
     3661 aacgaatacc tgctgcttgt gcaagttttg tttttgaacc gaaatacaaa agagcgtcag
     3721 ttttaagcat ttaaaacacc tttattgtta gtcataacta acaagataga tgttaacaaa
     3781 aacatagtca atacgattta gcattagcta actatggaaa caaaaaattt aactatcggc
     3841 gaacgcatca ggtatcgtcg gaaaaacctc aaacacaccc aaaggtctct tgctaaagcc
     3901 ctgaaaatct cccatgtgtc tgtatcacaa tgggaacggg gtgatagtga acctacaggg
     3961 aagaaccttt ttgccctcag caaagtattg caatgctcac caacatggat tctatttggc
     4021 gatgaagaca agcaaccaac accacctgtt gagaagccag ttgccttatc ccccaaagaa
     4081 ctagagctcc ttgagctgtt taatgcactg ccagaatcag aacaggatac ccagctcgcc
     4141 gaaatgcgag ctcgagtaaa aaacttcaat aaactctttg aagaattact aaaagcccgt
     4201 cagcggacaa ataaaagata acatcatcaa tgagttatct tttaccacat caattatgtt
     4261 agctatagca tacaaaatca cttgaccgat atgttagtca tggctaatct tgtttgcatc
     4321 aacacaccgc acggtgttct cagcaaacag ttccgctacc ccagcgttaa ggggaaatga
     4381 ggtcagcatg gatactatcg atcttggcaa caacgaatct ctggtgtacg gcgtgtttcc
     4441 aaaccaggac ggcacgttca ccgcaatgac gtataccaaa agcaaaacgt ttaaaaccga
     4501 aaatggtgcc cgtcgctggc tggaaagaaa ctcaggtgag tgatatggat ttcgacacaa
     4561 tcatggaaaa ggcttacgaa gaatacttcg aaggccttgc cgaaggcgaa gaagctctca
     4621 gcttcagtga gtttaaacag gcgctttcca gttcggcaaa atctaacggc tgataagcga
     4681 aacagcaccg cgaggaatca gtatgcagaa acgagaaccc gtcatcatcg cgccagacta
     4741 taccgatgat gaactttatg agtggatgcg ccagaaaatt aatgcagcgc aggatctgaa
     4801 atgggctaat gaagccaggg ctaagcaggc tgaaaatctg tccgctctgg agcaggatat
     4861 caccaatctg gaaaaagcag cggcattaag cattgccaga atgattacat acccgcgtta
     4921 gtagctaatc aacaaagcta aggttagtaa ttaaggagtt ctccacgggt gaggtggagt
     4981 gcgtgcgccg gacacgggtg cgcatccgga actgacagtt tactgaaagg atatttccct
     5041 gaaaagtcag accataacgc gaaagcgcac ggcgaggtag ctggttcata gatagcctgt
     5101 cgttaaattt tcgtcgaccg tgcgcttccg gttgtggcaa cccgcgaaat ggcgcggcgg
     5161 taagtatggc ggggttattc cttccccgtt gaggacaccg ggttgtcagg ttgaccatac
     5221 gcttaagtga caaccccgct gcaacgccct ctgttatcaa ttttctggtg acgtttggcg
     5281 gtatcagttt tactccgtga ctgctctgcc gcccttttta aagtgaattt tgtgatgtgg
     5341 tgaatgcggc tgaacgcacg cggaacagtt aaaaccaaaa acagtgttat gggtggattc
     5401 tctgtatccg gcgttaattg ttaactggtt aacgtcacct ggaggcacca ggcactgcat
     5461 cacaaaattc attgttgagg acgcgataat gaaaacgtta ttaccaaacg ttaatacgtc
     5521 tgaaggttgt tttgaaattg gtgtcactat cagtaaccca gtatttactg aagatgccat
     5581 taacaagaga aaacaagaac gggagctatt aaataaaata tgcattgttt caatgctggc
     5641 tcgtttacgt ctgatgccaa aaggatgtgc acaatgaatt cagcatttgt gcttgttctg
     5701 acagtttttc ttgtttccgg agagccagtt gatattgcag tcagtgttca caggacaatg
     5761 caggagtgta tgactgcagc aaccgaacag aaaattcccg gtaactgtta cccggtcgat
     5821 aaagttattc accaggataa tatcgaaatc ccggcaggtc tttaaaacag ttccgtaata
     5881 aatatccggt ttcattctta tatgccagca atggcaggga tttgttcatc cttaaatctg
     5941 tcatgaggtt aaaacaaatg agtaaagtct ttatttgcgc cgctattcct gacgaactgg
     6001 caacaaggga agaaggcgct gtggctgtag ccacagccat tgaagctggc gacgaacgcc
     6061 gtgctcgagc aaaatttcac tggcaattcc tggaacatta tccggctgct caggactgcg
     6121 cttataaatt tattgtctgc gaggataaac ctggcatacc ccgccctgcc ctcgattcat
     6181 gggatgctga atatatgcag gaaaaccgct gggatgagga gtctgcttct tttgtcccgg
     6241 ttgagactga atccgatccg atgaacgtca cttttgacaa gctggcccct gaagtacaga
     6301 acgctgtcat ggttaagttc gacacatgtg aaaacatcac cgttgatatg gttattagcg
     6361 cacaggaatt gttgcaggaa gacatggcaa cattcgacgg acatatcgtt gaagcgttga
     6421 tgaaaatgcc agaagttaac gccatgtatc cggagcttaa gttgcacgcc attgggtggg
     6481 ttaagcataa atgtattcct ggtgctaaat ggcccgaaat tcaggcagag atgcgcatct
     6541 ggaaaaaacg tcgcgaaggt gaacgcaagg aaaccggaaa atacacgtct gttgttgatc
     6601 tcgcccgcgc cagagccaat caacagtaca ctgaaaattc aacaggaaaa atcagcccgg
     6661 tcattgctgc cattcatcgc gaatacaagc agacatggaa aacactggat gacgaactgg
     6721 cctacgctct ctggcctagt gatgtggatg ccggaaacat tgacggcagc atccatcgct
     6781 gggcaaaaaa agaagttatc gacaacgacc gcgaagactg gaagcgtatc tcggcatcaa
     6841 tgcgcaaaca gcctgatgcc cttcgctacg accgccaaac tatttttggc cttgtccgtg
     6901 agcgtccgat cgacattcac aaagatcccg tagcactgaa caaatatatc tgcgaatacc
     6961 tgacgacaaa gggcgtgttt gagaatgaag aaacagacct gggcactgtt gatgttctcc
     7021 agtcatcaga aacacaaact gatgcagtgg aaactgaggt atctgatatc ccaaaaaatg
     7081 aaaccgcgcc ggaagctgaa ccatctgtag agcgtgaggg gccgttctat ttcctcttcg
     7141 cagataagga cggagaaaaa tacggtcgcg caaacaaact ctctggtctg gataaggcac
     7201 tggctgctgg cgccactgaa atcacaaaag aagaatattt tgcccgaaaa aatggcacat
     7261 acacgggctt accgcaaaat gtagataccg ctgaagattc agaacaacca gagccgataa
     7321 aagttaccgc tgacgaagta aacaaaatta tgcaggcagc caatatcagc cagcctgacg
     7381 ccgataagtt gcttgctgca tcacgtggtg aatttgttga agggattagt gacccgaatg
     7441 atccgaaatg ggttaagggg ctccagaccc gcgattctgt gaaccagaac cagcatgaat
     7501 cggaacggaa ctaccaaaaa gcggaacaaa acagtccaaa tgcgttacaa aacgagccag
     7561 aaacgaaaca gcctgaacca gtagcgcaac aggaagtgga aaaagtctgc accgcctgcg
     7621 gtcagaccgg cggcggcaac tgccctgatt gtggcgcggt gatgggcgac gcaacatacc
     7681 aagaaacatt cgatgaagag tatcaggttg aagttcagga agatgatccg gaggaaatgg
     7741 aaggcgctga acatccacac aaggagaaca ctggcggcaa tcagcatcac aatagcgata
     7801 atgaaactgg cgagacggca gatcacccaa ttaaggtgaa cggtcatcac gaaatcacat
     7861 ccaccagcag gacgtgtgac catctaatga tcgaccttga aaccatggga aaaaatcctg
     7921 atgccccgat catctcaata ggtgcaatat ttttcgatcc gcaaaccgga gatatgggac
     7981 cggaatttag taagactatc gatctggaaa ctgctggcgg agtcattgat cgggacacca
     8041 ttaaatggtg gcttaagcaa tcacgcgaag cgcaatctgc cattatgacc gatgaaatcc
     8101 cgttagatga tgcactgtta caattgcgag aatttatcga cgaaaactcc ggtgaatttt
     8161 ttgttcaggt ttggggaaat ggagccaact tcgacaacac gattttgcgc cgttcatacg
     8221 aacggcaggg gatcccctgc ccgtggcgtt actacaacga tcgcgatgta cgcacaatcg
     8281 ttgagctggg gaaagccata gacttcgatg ccagaacggc tattccattc gaaggtgagc
     8341 gccataatgc acttgatgac gcccgttacc aggcaaaata cgtttcagtt atctggcaaa
     8401 aactgatccc gagtcaggct gattcttaat gttcaactgt cgccggttgt gactggtatt
     8461 ctgcaaccgg cgctcgtctg atgtaagaga taaagaaatc gatgagcgaa gtaatcatga
     8521 ttgtctctcc cggcaaatgg gtatccgaag agcagttaat tgcgctgaaa ggaataaaaa
     8581 aaggtacgtt aaaaaaggcc cgggaaaaat cgtttatgga aggaagggaa tataagcatg
     8641 tcgctcatga cggtatgcca tgggataaca gtccatgctt ttacaacctg gaagaaattg
     8701 atcgctggat tgagcgccag gcatcagcga gaccaagacg tcatcttact tgactaaaag
     8761 ccacactaac taatgagaga agttgaaatg aaatatccga caggcgtgga aaaccatgga
     8821 gggaaattac gtatctggtt tgtttataaa gacgtaagag tcagggaaaa tctgggggtt
     8881 cctgacacag caaaaaacag gcgcgttgca ggtgaactac gctcctctgt ttgttacgca
     8941 ataaaaactg gtgttttcga ctatgcaaaa cagtttccct cctcacgcaa tctggaaaaa
     9001 tttggtgagg cccgacaaga tttaaccata aaagaactgg ctgaaaaatt tctggcactg
     9061 aaagaaactg aagtcgccaa aacatcactc aacacatacc gtgccgtcat caaaaatatc
     9121 ctgagcataa tcggtgaaaa aaatcttgcc tcatcgatta ataaagaaaa attactggag
     9181 gttcgtaaag agttactgac tggataccag atccccaaaa gtaactatat tgttacacaa
     9241 ccagggagat cggctgtaac tgtaaataat tacatgacaa atcttaacgc cgtgttccag
     9301 tttggtgttg ataacggtta cctggcagat aatccgttta aggggatctc gccattaaag
     9361 gaatcaagaa ccattccgga tcctctttcg cgggaagaat ttatccgtct tatcgatgcg
     9421 tgcagaaatc agcaagcaaa aaatttatgg tgtgtttctg tttatactgg agttcgccct
     9481 ggtgagctgt gtgcacttgg atgggaggac atagatctga aaaatggaac aatgatgatc
     9541 aggagaaatt tagcaaaaga ccgtttcacg gtaccaaaaa cacaggcggg aaccaatcgg
     9601 gtcattcatc ttattaagcc agcaatcgac gctctccgga gtcagatgac attaacgaga
     9661 ctgagcaaag agcatatcat tgatgttcac ctcagagagt atggcagaac agaaaaacaa
     9721 aaatgcacct ttgtttttca acctgaagtg tcagcgagag taaaaaatta tggtgaccat
     9781 tttaccgttg actcaataag gcagatgtgg gacgcagcga taaaacgtgc cggactccgc
     9841 catcgaaaat catatcagtc gagacatact tatgcctgct ggtcgctgac agctggtgct
     9901 aacccggcat ttatagcaaa ccagatgggc catgcagatg cgcaaatggt atttcaggta
     9961 tacggaaaat ggatgtctga aaacaataat gcacaggtag ctttgttaaa tacacagtta
    10021 agcgagtttg ccccaaccat gccccataac gaagcaatga aaaattaatt taatat
//
LOCUS       DAPHBJ010000162         9917 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.164, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000162 DAPHBJ010000000
VERSION     DAPHBJ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.164"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            6..81
                     /locus_tag="RHZ76_003520"
     tRNA            6..81
                     /locus_tag="RHZ76_003520"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="RHZ76_003521"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="RHZ76_003521"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(467..1225)
                     /locus_tag="RHZ76_003522"
     CDS             complement(467..1225)
                     /locus_tag="RHZ76_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDV2814105.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEDLAVHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1233..2336)
                     /locus_tag="RHZ76_003523"
     CDS             complement(1233..2336)
                     /locus_tag="RHZ76_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDV2814106.1"
                     /translation="MTKVLLSHPPRPASHNSNRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFALERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2346..3527)
                     /locus_tag="RHZ76_003524"
     CDS             complement(2346..3527)
                     /locus_tag="RHZ76_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDV2814107.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIEAIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3595..4620)
                     /locus_tag="RHZ76_003525"
     CDS             complement(3595..4620)
                     /locus_tag="RHZ76_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV2814108.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDICRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(5051..5272)
                     /locus_tag="RHZ76_003526"
     CDS             complement(5051..5272)
                     /locus_tag="RHZ76_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814109.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5525..8629)
                     /gene="acrF"
                     /locus_tag="RHZ76_003527"
     CDS             complement(5525..8629)
                     /gene="acrF"
                     /locus_tag="RHZ76_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HDV2814110.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            complement(8641..9798)
                     /gene="acrE"
                     /locus_tag="RHZ76_003528"
     CDS             complement(8641..9798)
                     /gene="acrE"
                     /locus_tag="RHZ76_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HDV2814111.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2627 a         2577 c         2388 g         2325 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aataaaacaa aaggctcagt cggaagactg ggccttttgt tttatctgtt gtttgtcggt
      301 gaacactctc ccaaccagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
      361 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc agacatcaaa tcaagcgaaa
      421 ggccatccga aaggatggcc tttttgcttt tcgaactaac attcaattaa tggattacct
      481 gcgataaaaa tgccctcgta cgctctgatt taggatgcgc aaaaaactca tcaggtgcag
      541 cttgttccac tatctcccca cgatccataa aaatcacccg gtcagcgacg gttcgtgcaa
      601 accccatctc atgtgttaca cacagcattg tcatacccga ctgcgccagc ccaatcatcg
      661 tatccagcac ctctttcacc atctcaggat cgagcgccga cgttggctca tcaaacaaca
      721 taattttcgg cttcatacac agcgaacgcg caatggcaac gcgttgctgc tgaccacctg
      781 aaatctgtcc gggaaactta tgcgcatgtt cggcaattct cacccgctcg aggtaatgca
      841 ccgccagatc ttcagcctct ttcttaggca tcttgcgtac ccaaatcggt gccagggtac
      901 agttctgtag aacggtcaga tgagggaaga gattgaaatg ctgaaagacc attcccactt
      961 cctgcctgac gcgctcaata ttgcggatat cttcattaag ttcgatgcca tctaccacga
     1021 tccgtccctg ttgatgttct tccagatgat taatacaacg aatggttgtc gatttaccgg
     1081 aacctgaagg gccacacaga acgatccgtt ctcccggttg cacggttaaa tttatatttt
     1141 tcaaaacatg gaattgtcca taccatttat tgacgttttc cagcgtaatc atcgcgttag
     1201 caggttgcag taaaatttgg ctcatagtgt cctcaatgcg gtgtacgccc ggtgttaaaa
     1261 cgtttttcca gatgctggct atagcgcgac atgctgaaac agaagatcca gtagatcagc
     1321 gcggcgaaaa catacccttc cgtcgacata cccaaccagg cgggatcaac ggttgcctgc
     1381 tgaacgctac tgaaaagatc gaacaacccg ataatgatca ccaggctggt atctttgaag
     1441 agtgcgatga tggtatttac cagcccagga attaccagct tcaacgcctg tggcagaata
     1501 accagcccct gagttttcca gtaacccaac gccagcgact ctgccgcttc atactgccct
     1561 ttaggcagtg cctgtaatcc gcctcgcacg acttccgcaa catatgctga ctgaaacagg
     1621 atcacgccaa ccagcgcgcg gatcagtttg tcgatactgg tgccttctgc cataaacaac
     1681 ggcagcatga ccgaagacat aaacaatacg gtaatcaacg gtacgccgcg ccagaactcg
     1741 ataaaaatga ccgataagat acgcacaatc ggcatatggg agcggcgacc taacgccagt
     1801 aagatccccc acggtagcgc ccccgcaatc ccaactgatg caataattaa agttagcgtc
     1861 agcccgcccc attgccgggt ttcaacccgc tcaagagcaa aaaaaccgcc atacatcagc
     1921 caccagacaa tcagtgggta aatcaccgcc caggccgcaa tatagcgacc gcgatgcggg
     1981 agtattttcc agaacattgg tgcgatcgac acaagcccaa tcagtaatgc gaggttaatt
     2041 cgccagcgtt ggtcgtgtgg gtaaagccca tacataaact gaccaaatcg ttcgtggatg
     2101 aagacccaac aggcaccggc ttttgtgcag tcggcacgcg ttgagccaac ccagttagcc
     2161 tgcaaaaatg cccagttcag caacggtggg atcagttccc acatcaacca tatgcagcca
     2221 atagtcagca ggctattgct ccagctggag aacagatttt ttcgcaccca caccatcgcg
     2281 cggtttgagt tatggctcgc cgggcgcggg ggatgagaca gcaatacttt tgtcatcatg
     2341 actccttagc gttcaacgat cgcgatgcgg cggttataga tattcatcag cagcgagata
     2401 gtcaggctga taatcagata gaccgacatg gtcatggcta tcgcctcgat ggcttgcccc
     2461 gtctgattca gcacggtgcc ggcaaacagc gaaaccatat cgggatagcc aatagcggcg
     2521 gcaagagagg agtttttgac gatgttgaga tactggctgg ttaacggtgg aataatcacc
     2581 cgcaatgcct ggggaataat gacctggcgt agcgtaaccg ggttgggtaa tcccagtgac
     2641 cgagccgctt catgttgacc ataaggcact gcctggatcc cagcgcggat aatctcggcg
     2701 ataaatgcag aggtataaac cgaaagtgcc agcgttaagg ctgccagttc aggaattaaa
     2761 accatcccgc cgcggaagtt aaagcctcgt agggctggga catcccagtg tagtgctgcg
     2821 ccaaaaagcc attgcgccag taaaggcaaa ccaatgatca acactgcggc gatcggccag
     2881 gtgcggcgca gttgtccggt ctttatctgg tatgttttat taaagcggaa tagcccaaca
     2941 gaaagaacta tagccataac aacagccagg ataaacgcaa taaatccgtc tcccagctgc
     3001 ggtgacggaa tataaagccc acgattgctc aaaaaggcca gatcaaacgc gctcacagcc
     3061 tggcgtggtc cgggcaaatt gcgcaacacg gcaaagtacc agaagaagat ttgcagcagc
     3121 gggggaatat tacggaagat ctcaatataa attgtggaaa gctttcgtag cagccagtta
     3181 tccgaaagtc tcgccagacc gataaagaag cccaaaacag aagcgaacac aatacacaat
     3241 gcagaaacca gtagcgtatt gagtaagccg acaataaaaa cgcgtccgta ggtgtcgccc
     3301 tgctggtaat cgatcaaatg ctggacaata ccgaagccag cgccgcgatc cagaaaggca
     3361 aaacctgaag taatgccacg attattgaga ttcgttacag tgttgtgaaa taaccaacca
     3421 acaatgccga caacagcaac aacggcaagg atctggaata accaggcacg aaccgtaggg
     3481 ttggcaaagg agagtgagcc tttaacggtt gagcggcgat gagacataga aacctcggaa
     3541 agcataacgc tgaagagagc gccgccgtcg cgacgctcgt tacctcacac cgacttaacg
     3601 cacgggcggt gcgtactgaa taccgccgtt attccagaga ttattttgcc cacgtttaat
     3661 tttcagcggg ctttctgaac ctacgttacg ctcaaaaatt tccgagtagt tacccacctg
     3721 tttgatgatg ttataggccc atttattatc caacttcaga tccttgccgt aatcgccttc
     3781 tttacccagc agatgtgcca tatcaggcgt tgctggatta gccgcttttt catcaacgtt
     3841 ctgggaattg atgcccatct cttcagcatt cagcatggcg aaaagcgtcc agcgtacaat
     3901 cgagaaccat tcatcatcgc cacgacgaac taccggccca agcggttctt tagagataac
     3961 ctccggtaag acaatccatt cagctgggtt gcttaatttg atgcgcaggg catacagttg
     4021 cgactgatcc gaggccagcg tatcgcagcg accagattcc agtgccttcg ctgattcgtc
     4081 agagcgatcg aaagtcaccg gtgtgtactt catattgttt gccttgaaat agtcggcaac
     4141 gttgagttcg gtatcagtac ccgcctgaat acagacggta gcgccatcca gttctttcgc
     4201 gctttttagc cccgctttat cgtgcgtcag gaagccaatg ccgtcgtaat aagtgacgcc
     4261 agtaaatgcc attcccatcc cggcgtcgcg agatgaagtc caggtcgtat tacgggagag
     4321 caaatccact tcccctgact gtaaagccgt gaagcgttct tttgctgtga gtggggtata
     4381 tttcactttc gtgtcgtcac caaatacagc agcggcaaca ccacgacaaa tatcaacatc
     4441 aatacctgaa aatttaccgt cagcatcggc ataagagaac ccaggtaatc catcactgat
     4501 cccgcattgc acaaaacctt ttttctgtac ggcatcaagc gtcgcgccag catgcgcctg
     4561 atttgcaacg gcaagcagca cgctggcggc agccagtgtg gctatcatca tctttttcat
     4621 aatgcttcct gtgaggcgaa aattatcgtt ataggacgtt tgggaaacgt cttttacctg
     4681 tctgtggcca tcctcttata agcaaaagta gtgccagctt ttgcgcacga tttcgcgtcg
     4741 caataagctg agtgtagaca ggaaataata aaaacagcgc cccgcaatgg tgcgaaaatg
     4801 aaacatgcgc cacattttgg cgcaaaatat ttacgcgtgt tttactaagc agaataatga
     4861 ttgttagcgt gagtatattt ctggtgagaa gtgtgaaagt taaattgatg cgttatttcg
     4921 aaagcagcga aataacgcga tataacatgc taaatcagca gagaattagc cagcagaata
     4981 tagcaatatt ttatcttatg taaagatact gtatcgttaa acagagcgcg aaacttcgcg
     5041 ccctgttcag ttatttcgac agagcgatga tccccatcgt caggccagca acagctgctg
     5101 ctccgccagc aatagctcca gccgtttccc agttatcctg agttgtgcct ttcatacagg
     5161 tattggtgtg aaccagacgc ccctgatcgt cataaaccgg aacgcagggc gaatcgtgag
     5221 cacagcccgc cagcaatgcg gtgagcaatg ccacaggaat taatcttttc atggtgttac
     5281 ctcagtaata aacgctaacg tttaataagt taactctctt attaacgagc tgaatgccat
     5341 tttatcaccg gaggttataa atcttgcgga atgaataatg acaaattatg tgaattgtaa
     5401 aaatagtgaa gggagcggga actatagaaa gcttttaaag agtgagcaaa agaatatctt
     5461 attaattttt atgggtttca ctgaaaataa caaaggcacc cgaaagcgcc tttatgtttc
     5521 agatttatcc tttaaagcaa cggcggatca ccacaaagaa caccggtacg aagaagattg
     5581 ccagcaacgt tgcagagacc attcctccca ttaccccgat acccactgcg ttctgcgcgc
     5641 cactgccggc accgttactg atagctagcg gtaatacgcc gagaataaag gcgagagagg
     5701 tcatcaggat aggacgcaga cgcatacgta ctgccatcag tgtcgcttca acaacacctt
     5761 taccctcttt ctccatgaga tctttagcga actcaacaat caaaatagcg tttttggccg
     5821 acaagccaat tgtcgttagc aagcccacca taaagtagac gtcatttttt tgattaaaga
     5881 gtgtcgccgc cagcagcacg ccgacaatcc ctaacggcac gaccaacata accgagacag
     5941 gaattgacca gctttcatag agtgcagcaa ggcacaggaa aacaaccaca aaggaaattg
     6001 ctaccagagc gggagcctgg tttcccgata agcgttcctg atacgacata cccgtccagt
     6061 cataaccaat gcccgcaggt aattttgacg caaggttttc catcaacgcc atggcatcgc
     6121 cggaactggt tcctggcgcg gcttccccct gaatctccat tgacggcaga ccgttgtagc
     6181 gttccagtcg cggagagcca tacacccaat gtgaagtggt aaaggcagag aatggcacca
     6241 tttcgccgtt ggcgctgcgg acataaagtt tatcgacatc ttctggcagc atacggaatt
     6301 tggcatccgc ctgaacatac acctttttca cacgaccacg gtcgatgaag tcgttaacgt
     6361 aagtcccacc cagcgccgtt gaaatggtct gattgatgtc agaaagtgaa acacctaatg
     6421 cctgcgcctt ttcctggtca acttccagtt taaactgcgc ggtgtcttcc aggccattag
     6481 gacgcacgct gactaagctg gcaggatgtt gcgcagccat accaagcaac tggttacggg
     6541 cctgggttag ggcatcgtga cccagcccag cctgatcaat taactcaaag tcgaaacccg
     6601 ttgccgtgcc cagttcaaca atggctggca tattgaatgg aatgacaaaa ccgtcgcgga
     6661 tcttgcccaa ttccattttg gcacgatgga ttaccgcttc cgcactgttt tcatcaccat
     6721 tacgctcttc ccacggtttc agactgacga aggccatacc ggcgttttgt gcctggccgc
     6781 tgaagctaaa gccgttaacc gtaaagacac tttcaacgtt cgctttctcg tttttcagat
     6841 agtaatccgt cacttgatcc aacacttttt gcgtccgctc ttgcgtcgcg ccagcgggta
     6901 actgaatcat ggtcagaaag acaccctgat cctcttcagg taagaaggaa gacggaagac
     6961 gtaaaaacaa caccaccatt cctgcaacaa tcagcgcata gatcagtaaa tatcgtcctg
     7021 tggaaccgag gattttgccg acgctgttgg tgtagtggtt aacgctatga tcgaaggtgg
     7081 tattaaacca gccgaagaaa ccgcccttat tttcgtgatg ctcagcagag acgggtttaa
     7141 gcagcgttgc acataacgca ggggtaagaa tcaatgccac cagaacagaa agcgccattg
     7201 ccgaaacgat ggtgatagag aactggcgat aaattgcccc agtagaaccg ccgaagaatg
     7261 ccatcggaat aaataccgct gacagcacca tcgcgatacc caccagtgcg ccctgaattt
     7321 gcgacattga tttttccgtc gcttcttttg gcgggagctt atcctccatc atcacgcgct
     7381 cgacgttctc caccaccact atcgcatcat cgacgagcag cccgatggca agcaccatcc
     7441 cgaacatcgt tagtgtgttg atggagtaac caaaagcggc gaggatggca aacgtcccta
     7501 acaacaccac gggtaccgca atggtgggga tcagcgttgc tcgcatattc tgcaagaaca
     7561 gatacatcac caggaacacc agcataatgg cttcgaacag cgtttttacc acttcgtgaa
     7621 tagaaagctg gacgaatggc gtggtgtcat aagggtagag aaccttcatt ccctgcggga
     7681 agaatggctg taattccgcc agttttgcct taatggcttt cgcggtatcg agagcattcg
     7741 cgccggttgc cagcttaatc cccaggcccg ccgccggttt tccgttgatg cgagcgataa
     7801 cgttatagtt ttcaccgcca agttcaaccc gtgcgacatc tttcagacgc accaccgagc
     7861 cgtcactgtt tacgcgcagg gtcactttgc cgaattcttc cggatttttt aaccgcgtct
     7921 gagcaataat cgaggcattc aactgttgcc ctggtaacgc tggcgttccg cccaactgtc
     7981 cggcagcgat ctgatcgttc tgtaccttca actggttaat cacatcaacc ggtgtcagtt
     8041 tatatttgtt tagcagatcg gcgtccagcc agatacgcat cgcatactgt gcgccgaaaa
     8101 gctgtacgtc accgacgcca ttcagacggc taagcgtatc tttaacgtta gaagccacat
     8161 agtccgagat atcgtcctgt gtggtgtctg ggttatcaga gacaaagccc gccaccatca
     8221 aatagctgct actggacttt tcaacactga tcccctgctg ctgaacttcc tgcggcagca
     8281 acggcgtggc gagctggagt ttgttctgca cctgcacttg cgcgatatca ggatcggtcc
     8341 cggactggaa ggtaagggta attgtcacgc taccggcgga atcgctggtg gaggacatat
     8401 acatcaggtt atcgatacca ttcatattct gttcgataac ctgcgtcacc gtatcctgca
     8461 cggtctgcgc atcagcacct ggatagtttg ccgaaacaga aaccgcaggc ggtgcaatgg
     8521 ttggatactg agcgacgggc aattgtagga tcgccagtgc gcccgccatc atcagaataa
     8581 tggccagcac ccatgcaaat atcggtcgtc gaataaaaaa gtttgccatg tcagattacc
     8641 ttacttcgat gcagtatctg cgggggtatc ggtagtggct ttaacctgct ctcccggacg
     8701 cgctttttgc aggccgctga caatgacttg atcgccagat ttcagacctt cgctaatcaa
     8761 ccatttatcg ccaatcgcct gactggcaac gacagggcgc gcttccacct gacttttatc
     8821 gttaacaatc agcacggttg catcaccacg cggtgtgcgg ctaacgcctt gttgcgggat
     8881 aagaatggcg tcaggttgga cgccttcatc aatccgtgca cgcacaaaca tacccggcaa
     8941 aagcgtatgt tgcgggttag ggaagacagc acgtagggtt atggagccgg tgctttcatc
     9001 aacggtcaca tcagagaatt gcagcgtacc tttcagggga taggtttgac cgttttccat
     9061 gaccaactct acgttgctgg tggcgttttc cttatgcaaa tttccttgct ctacggattg
     9121 cttcagcctc ataaaatcgt tgctggattg ggtcacatca acgtagatag gatcgagctg
     9181 ctggacagtc gccagttcag tcgtttgccc attagtgaca agagcgcctt cggtcacggt
     9241 cgatttgccg atacgtccgc taattggcgc agtcactttg gtataagcga gattgatgcg
     9301 agcgctttcg actgtggctt ttgcggcaat cacggcggca tcggcctggc gagcatcagc
     9361 aatggcctgg tcgtactcct gctggctgat gtatttcgta cccacgagcg gaacgtaacg
     9421 ttttaccgtc aaatgcgcga tggcggcggc ggcttcactt ttcgccagtt cgcctttcgc
     9481 gctgtcataa tttgcctgat aggtcgcggg atcgatctgg tacagggact ggcctgcttg
     9541 tacatcgctg ccttcagtga aattacgatt cagtacgatc ccgctaacct gtgggcgaac
     9601 ttcggctata cggtaagcat tggtgcggcc tggtaattca gtcttaactt ctaacggggc
     9661 cgttttcaca atatgaacgg taacctgcgg ttcaccgacg tgagctttct cttctccctt
     9721 atcgttacaa ccggcgatta aagccgcgga gatcagaata aaggagggca ggaggaaaaa
     9781 cctggcatgt ttcgtcatta ctattcctca aaaaaccaaa agcgcgttat ttacccagaa
     9841 aggcaacgcg ttaactcgca gaaagaaaaa tacagtttgc tatcctacaa attatcattc
     9901 gtcgatgtaa ggaatag
//
LOCUS       DAPHBJ010000163         9878 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.165, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000163 DAPHBJ010000000
VERSION     DAPHBJ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.165"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(156..626)
                     /gene="bcp"
                     /locus_tag="RHZ76_003529"
     CDS             complement(156..626)
                     /gene="bcp"
                     /locus_tag="RHZ76_003529"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDV2814112.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(626..1198)
                     /locus_tag="RHZ76_003530"
     CDS             complement(626..1198)
                     /locus_tag="RHZ76_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HDV2814113.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            1344..2222
                     /gene="dapA"
                     /locus_tag="RHZ76_003531"
     CDS             1344..2222
                     /gene="dapA"
                     /locus_tag="RHZ76_003531"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDV2814114.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            2239..3273
                     /gene="bamC"
                     /locus_tag="RHZ76_003532"
     CDS             2239..3273
                     /gene="bamC"
                     /locus_tag="RHZ76_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HDV2814115.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            3486..4199
                     /gene="purC"
                     /locus_tag="RHZ76_003533"
     CDS             3486..4199
                     /gene="purC"
                     /locus_tag="RHZ76_003533"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDV2814116.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVEFVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            4367..5230
                     /locus_tag="RHZ76_003534"
     CDS             4367..5230
                     /locus_tag="RHZ76_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HDV2814117.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            5245..7260
                     /gene="tmcA"
                     /locus_tag="RHZ76_003535"
     CDS             5245..7260
                     /gene="tmcA"
                     /locus_tag="RHZ76_003535"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HDV2814118.1"
                     /translation="MAELTALYILTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDTDSLRWSDCPDPIATPHFVQHFKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNNVRTERLRDRITQWQFFH"
     gene            7334..8032
                     /gene="ypfH"
                     /locus_tag="RHZ76_003536"
     CDS             7334..8032
                     /gene="ypfH"
                     /locus_tag="RHZ76_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDV2814119.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(8141..8341)
                     /locus_tag="RHZ76_003537"
     CDS             complement(8141..8341)
                     /locus_tag="RHZ76_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HDV2814120.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(8369..9496)
                     /gene="dapE"
                     /locus_tag="RHZ76_003538"
     CDS             complement(8369..9496)
                     /gene="dapE"
                     /locus_tag="RHZ76_003538"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HDV2814121.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(9500..9856)
                     /locus_tag="RHZ76_003539"
     CDS             complement(9500..9856)
                     /locus_tag="RHZ76_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HDV2814122.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         2336 a         2467 c         2713 g         2362 t
ORIGIN      
        1 tggattctga acggtacaaa aatgatggtc attaacgttc agcaaaaaat ggattcgaca
       61 cccgtgtcgc aggggaaagc gagaatgtga tcttgatcac gagaagtgag gtatgctggc
      121 cgcaagcgca gccagcacgg aatggagcaa agtaatcagg cgtgttcttt cagccagttc
      181 agcacaacgt cgtggtgatt gctggttttg aaatcgtcaa agacatgttc gattttgcca
      241 tcagcgtcaa tcaggaagct gatgcgatga atgccatcgt aggttttgcc catgaaggac
      301 ttttcacccc agacgccgaa ttgttcgcac acctggtggt cctcatcaga caggagcgta
      361 aagttaagca gctctttttc cgcaaaacgg gagagttttt cgggtttatc ggtgctgata
      421 cccagcacat caacgcccgc ttttttcaac tcatccatgt tatcgcgtaa gccgcaggcc
      481 tgtacggtac agccgggggt catggctttc gggtagaaat aaaccagaac acgctgtccc
      541 tggaagtcgg tcaaattaac ttgttctccg tcttgatccg gcaagctaaa tttcggtgcg
      601 atatcaccgg ctttcagtgg attcattact taactccatc ctgttcatca tgttgggaat
      661 aattgacgac gttaatactg ccttgtgcat tgagttctgt acatagggct ttgaacgctt
      721 gctcaatatt tgctgcgtcc gcagatgcgg ggctgtgggc ggttatctga atatgcaact
      781 gcgcagcccg ttcattttca gcaggttgcg tgcgcgacac cagctccgca atgttcatat
      841 gatgcgcgtc gaaaagtgct gtgaagcgtt caattaaatg cggggagtct gccacatcga
      901 cctgaaccca gacagatgct ggcattggcg gacgcggacg cgccgtcgtg cgcttcatca
      961 cgattaaaag atccagttcg gcacctttca acggtaacgt tgattcaatc agagtaatgg
     1021 cattccatga accggaaagc agcataataa acgtgaactc ttctcccagc atcgccaggc
     1081 gactgtcttc aatattacag ccgcaactac tgacatgacg ggtgatggtg ttcacaattc
     1141 cagggcgatc ggcacccaac gcagtgatca ccagataatg ttgcgatgac agtgtcaaac
     1201 tggttattcc tttaaggggt gagttgttct taaggaaagc ataaaaaaaa catgcataca
     1261 acaatcagaa cggttctgtc tgcttgcttt taatgccata ccaaacgtac cattgagaca
     1321 cttgtttgca cagaggatgg cccatgttca cgggaagtat tgtcgcgatt gttactccga
     1381 tggatgaaaa aggtaatgtc tgtcgggcta gcttgaaaaa actgattgat tatcatgtcg
     1441 ccagcggtac ttcggcgatc gtttctgttg gcaccactgg cgagtccgct accttaaatc
     1501 atgacgaaca tgctgatgtg gtgatgatga cgctggagct ggctgatggg cgcattccgg
     1561 taattgccgg gaccggcgct aacgctactg cggaagccat tagcctgacg cagcgcttca
     1621 atgacagtgg tatcgtcggc tgcctgacgg taacccctta ctacaatcgt ccgtcgcaag
     1681 aaggtttgta tcagcatttc aaagccatcg ctgagcatac tgacctgccg caaattctgt
     1741 ataatgtgcc gtcccgtact ggctgcgatc tgctcccgga aacggtgggc cgtctggcga
     1801 aagtaaaaaa tattatcgga atcaaagagg caacagggaa cttaacgcgt gtaaaccaga
     1861 tcaaagagct ggtttcagat gattttgttc tgctgagcgg cgatgatgcg agcgcgctgg
     1921 acttcatgca attgggcggt catggggtta tttccgttac ggctaacgtc gcagcgcgtg
     1981 atatggccca gatgtgcaaa ctggcagcag aagggcattt tgccgaggca cgcgttatta
     2041 atcagcgtct gatgccatta cacaacaaac tatttgtcga acccaatcca atcccggtga
     2101 aatgggcatg taaggaactg ggtcttgtgg cgaccgatac gctgcgcctg ccaatgacac
     2161 caatcaccga cagtggtcgt gagacggtca gagcggcgct taagcatgcc ggtttgctgt
     2221 aaagtttagg gagatttgat ggcttactct gttcaaaagt cgcgcctggc aaaggttgcg
     2281 ggtgtttcgc ttgttttatt actcgctgcc tgtagttctg actcacgcta taagcgtcag
     2341 gtcagtggtg atgaagccta cctggaagcg gcaccgcttg cggagcttca tgccccggct
     2401 ggaatgattt tgccggtgac ctccggtgat tatgcaatcc cggtgaccaa cggtagtggt
     2461 gctgtcggta aggcgctgga cattcgtcca ccagcccagc cgctggcact ggtttctggc
     2521 gcgcgtaccc agttcacggg cgataccgct tcattgctgg tggaaaatgg tcgtggcaat
     2581 actctgtggc cgcaggtggt tagcgtgctg caggcgaaaa actacaccat cacccaacgt
     2641 gatgatgctg gtcagacact gaccaccgat tgggtacaat ggaaccgtct ggacgaagac
     2701 gagcagtatc gtggtcgtta tcaaatctct gttaagccgc agggttatca gcaggcggtt
     2761 acggttaaac tgctgaacct ggaacaggcg ggcaaaccgg ttgcagacgc ggcttccatg
     2821 cagcgttaca gcacggagat gatgaacgtt atttccgccg gtctggataa atctgccact
     2881 gacgccgcga acgctgcgca aaatcgtgcc tccaccacta tggacgtaca aagtgcagct
     2941 gatgacaccg gtttaccaat gctggtcgta cgcgggccgt tcaatgtggt ttggcaacgt
     3001 ctgccagcgg cgctggaaaa agtgggcatg aaagtgaccg acagcacccg ttcgcagggc
     3061 aacatggccg taacttataa gccgctgtct gacagcgact ggcaggaact gggcgcgagc
     3121 gatccaggcc tggcatccgg tgactataaa ctgcaggttg gcgatttaga taaccgcagc
     3181 agcctacagt tcatcgatcc gaaaggtcat actctgactc agagtcagaa cgacgcgctg
     3241 gtagctgtct tccaggctgc gtttagcaag taaaaataca gggctggaat catccggccc
     3301 ttttttctga tatgatacgc aaacgtgtgc gtctgcagga aaacgcgatt ttagcggtaa
     3361 ttcgcacgaa atttgtttgt cggacgtagt tcggataagg cgttcacgcc gcatccgaca
     3421 aaacatccgg cacaccagac agcaaaagat tttaaaacgt taattcacac ccaggagtga
     3481 taaagatgca aaagcaagct gagttgtatc gtggtaaagc gaaaaccgta tacagcacgg
     3541 aaaacccgga cctgttggtg ctcgaattcc gcaatgatac gtcagcaggg gatggcgcgc
     3601 gcattgagca gtttgatcgc aaaggtatgg tgaacaacaa gttcaactac ttcattatga
     3661 gcaaactggc tgaagcgggt atcccgactc aaatggagcg tctgctctcc gataccgaat
     3721 gtctggtgaa aaagctggat atggtgccgg ttgagtttgt cgtgcgtaac cgtgctgctg
     3781 gctctctggt gaaacgtctt ggaatcgaag aaggtattga gctgaacccg ccgctgttcg
     3841 atctgttcct gaaaaacgac gccatgcacg atccgatggt caacgaatct tactgcgaaa
     3901 cctttggctg ggtgagcaaa gagaacctgg cgcgtatgaa agagctgacc tacaaagcga
     3961 acgacgtgct gaaaaaactg ttcgatgatg ctggtctgat tctggtcgac ttcaagctgg
     4021 agtttggtct gtacaaaggt gaagtggtac tgggcgatga gttttccccg gacggcagcc
     4081 gcctgtggga caaagaaacg ctggagaaaa tggacaaaga ccgtttccgc cagagcctcg
     4141 gtggcctgat cgaggcctat gaagccgtcg cccgccgcct gggtgtacag ctggactgat
     4201 ttttctgttc atcatcttgc cgtgcagctg gcacggcaag acaaccgctc tcggaatata
     4261 tgcattgaac gctcattcct ccgccatttc atcccgcttt cctgtggtaa tcttcctctg
     4321 aaccacctac gatcatttct ataatgaata tattgttgag gtatctatgc gttggcaagg
     4381 gcgacgtgaa agtgacaatg ttgaagacag gcgcaacagc tctggtggtc cgtctatggg
     4441 cggtcccggt tttcgcctgc cgagcggtaa aggcgggctg attttactga tagtcgtgct
     4501 ggttgcaggc tactatggcg ttgatttaac cgggttgatg accgggcagc cggtttctca
     4561 acagcaatca acgcggtcaa ttagcccaaa tgaagacgaa gccgcaaaat tcacctcggt
     4621 gattctggca accacggaag acacctgggg acaacagttc gaaaagatgg gtaagaccta
     4681 tcagcaaccg aagctggtca tgtaccgtgg aatgacgcgc accggctgcg gggcgggcca
     4741 gtccattatg gggccgttct attgcccggc ggatggcacg gtttatatcg atctctcctt
     4801 ctatgatgac atgaaagaca aacttggcgc ggatggcgat tttgcccagg ggtacgttat
     4861 cgcccatgaa gtcggtcatc atgtgcagaa actgttaggc atcgagccga aagttcgtca
     4921 actgcaacaa aacgcgacgc aggcggaagt aaaccgctta tctgtgcgta tggaactcca
     4981 ggccgactgt tttgccggtg tctgggggca tagtatgcag cagcaaggcg ttctggaaac
     5041 tggcgatctg gaagaggcgc tgaatgcggc gcaggccatc ggcgatgacc gtttacaaca
     5101 gcaaagtcag gggcgagtag taccagacag tttcacacat ggcacttctc agcaacgcta
     5161 cagctggttt aaacgtggtt tcgacagcgg cgatccggca caatgcaata cttttggtaa
     5221 aagcatttaa tttccggggc agggatggct gaactgactg cgctttacat attaacagcg
     5281 caaatgaaac gtgaagggat ccgccgcttg ctggtgttga gcggggaaga gggttggtgt
     5341 tttgatcatg cgcttaagtt gcgtgatgcc ttacctggcg actggctgtg gatttcgccg
     5401 cagccagatg ctgaaaacca ctgttcaccc tcggcgctac aaactttact tgggcgcgag
     5461 ttccggcatg cggtattcga cgcccgccac ggctttgatg ccgctgcctt tgcggcactt
     5521 agcggaacgt tgaaagcggg aagctggctg gttttgttac tccctgtatg ggaagagtgg
     5581 gaaaaccaac ctgataccga ctcgctgcgc tggagtgatt gccctgaccc tattgcgacg
     5641 ccgcattttg tccagcattt caaacgcgta cttacggcgg ataacgacgc tatcctctgg
     5701 cggcaaaacc agccgttctc gttggcgcat tttactcccc gtactgactg gcaccccgcg
     5761 accggcgcac cacaaccaga acaacagcaa ctcttacagc agctactgac catgccgccg
     5821 ggcgtggcag cggtaacggc tgcgcgtggg cgcggtaagt cggcgctggc agggcaactc
     5881 atttctcgta ttgcgggcag tgcgattgtc accgcgcccg caaaagcggc aacggatgta
     5941 ctggcacaat ttgcgggcga gaagtttcgc tttattgcgc cggatgcctt gttagccagc
     6001 gatgagcaag ccgactggct ggtggtcgat gaagccgcag ccatacctgc gccgttgttg
     6061 catcaactgg tatcgcgttt tcctcgaacg ttgttaacca ctacggtgca gggctacgaa
     6121 ggcaccggac gtggtttttt gctgaaattt tgcgctcgct ttccgcattt acaccgtttt
     6181 gaactgcaac agccgatccg ctgggcacag ggatgcccgc tggaaaaaat ggttagtgag
     6241 gcactggttt ttgacgatga aaacttcacc catacaccac aaggcaatat cgtcatttcc
     6301 gcatttgaac agacgttatg gcgaagcgat ccagaaacgc cgttaaaggt ttatcagtta
     6361 ttgtctggtg cgcactaccg gacctcgccg ctggatttac gccggatgat ggatgcacca
     6421 gggcaacatt ttttacaggc ggctggcgaa aacgagattg ccggggcgtt gtggctggtg
     6481 gatgagggcg gattatctca agaactcagt caggcggtat gggcaggttt tcgtcgcccg
     6541 cggggtaatc tggtggccca gtcgctggcg gcgcacggca gcaatccact ggcggcgaca
     6601 ttgcgcggaa ggcgggttag ccggatagca gttcatccgg ctcgtcagcg ggaaggcaca
     6661 gggcggcaac ttattgctgg agctttgcaa tatacgcatg acctcgacta tctttcagtg
     6721 agttttggtt acaccgggga gttatggcgt ttctggcaac gctgcggttt tgtgctggtg
     6781 cggatgggta atcatcggga agccagcagc ggttgctata cggcgatggc gctgttaccg
     6841 atgagtgatg cgggtaaaca gctggctgaa cgtgagcatt accgtttacg tcgcgatgcg
     6901 caagctctcg cgcagtggaa tggcgaaacg cttcctgttg atccactaaa cgatgccatc
     6961 ctttctgacg acgactggct tgaactggcc ggttttgctt tcgctcatcg tccgctatta
     7021 acatcgttag gttgcttatt gcgtctgcta caaaccagtg aactggcatt accggcgctg
     7081 cgtgggcgtt tacagaaaaa cgccagtgac gcgcagttat gtaccacact taaactttca
     7141 ggccgtaaga tgttactggt tcgccagcgg gaagaggccg cgcaggcgct gttcgcactt
     7201 aataatgttc gcactgagcg tttgcgcgat cgcataacgc aatggcaatt ttttcactga
     7261 ctccttcaat tttctcccat ggtcattgtg agtcagcggc gtaaaattaa tgacatcagt
     7321 taaggagttc gccatgaaac atgaccattt tgttgttcaa agcccggata aacctgcgca
     7381 acagttgtta ctgcttttcc acggtgtcgg ggataacccg gtggcaatgg gggaaatagg
     7441 cagctggttt gcgccgctat ttcctgatgc gctggtggtg agtgtcggag gcgcggagcc
     7501 gagcggtaat ccggcggggc gtcagtggtt ttcggtgcag ggtattacgg aagataatcg
     7561 ccaggcgcgt gtggatgcca tcatgccgac gtttattgaa acggtgcgct actggcagaa
     7621 acagagcgga gtaggggcta acgccacggc gctaatcggt ttctctcaag gcgcgattat
     7681 ggcgctggag agcattaaag ccgaaccggg ccttgcttca cgcgtcattg cttttaacgg
     7741 acgctatgcg agcctgccgg aaaccgcgtc tacagccacg acaattcact tgattcatgg
     7801 tggtgaagat ccggttatcg atctggcgca tgcagttgcg gcgcaggaag cgttaatcag
     7861 tgccggtggt gacgtgacgc tggatatcgt ggaggattta ggccatgcga tcgacaatcg
     7921 cagtatgcaa tttgccctgg atcacttgcg ttataccatt ccgaaacatt atttcgacga
     7981 agcgctaagt ggcggtaagc ctggcgatga tgatgtgatt gaaatgatgt aataatagcc
     8041 gggtaggcct gataagacgc gtgagcgtcg catcaggcat cgttcaccat tgccggatgc
     8101 ggcgtgaacg ccttatccgg cctacggttg agcactcagc ttacttcttc ggttgatcct
     8161 ttttcggcca gtcgtcgtca tcgtcccact tatcgttgaa atcacgatgc gggggaagct
     8221 ccggcttgtt ggcgagaaat ttcttatggt cgacgcgctt gagatcttta atcacgttca
     8281 gcaggacgcc taccaaaaag acaatcacca gaatccacca atatttagcc agccagtcca
     8341 tgcttatttc ctcttgcaga accactcatc aggcgacgag ctgttccatg atacgttgat
     8401 acatacgggc aagtagctgc aggtcggcag cgttcacaca ttcattaatt ttatgaatag
     8461 tggcattgac cggcccgagt tccaccacct gcgcccccat gcgagcaata aagcgcccgt
     8521 cggacgttcc gcctgtggtc agtagctgcg gtttaatttc attatagtgc tcaaccgcgt
     8581 taacgaccgc atccaccagt ttaccgcgcg cggtcaaaaa tggctgcccg gaaagccacc
     8641 aatccaccgt atagcgcagt tgatgttttt caagcagggc aagcacctgc gctttgatca
     8701 tctcatcagt cagttcggtg ctgaagcgga agttaaactg cacaaacagt tcacccggaa
     8761 taacgttgtt actgcccgtt cccgcctgaa tattggcaat ctgcatactg gtcgccggga
     8821 agaattcatt gccctgatcc cactcaatag ccactaattc attaaggaaa ggtgctgcgc
     8881 gatgtaccgg attgtcagcc agatgtgggt aggccacatg cccctgcacg ccatgaatgg
     8941 taaggttgca ggttaacgat ccgcgacgac catttttcac cacatcacct accacttcga
     9001 tactcgacgg ttcgccaacc aggcagtaat cgagacgctc attacgtgcc attaacgctt
     9061 cgacgacttt taccgtaccg ttgtgggcac tggcttcttc atcagaggtg atcagaaatg
     9121 ccagtcgccc cgtatggttg ggatgttgtg cgacaaaacg ttctgccgct accaccatcg
     9181 ccgccagcga gcctttcata tctgccgcac cgcgcccgaa taacataccg tcacgaatgg
     9241 ttggttcaaa cggcggattg atccaacgat cggcgtcgcc aggcggcacc acgtcggtat
     9301 gcccggcaaa ggctaacgtt tcaccctgcc cacgccatgc ccaaaaattc tgcgtatcgg
     9361 caaaatccat gcgttcaacg gtaaaaccga tcgcctgcaa acgttcaatc aacaaagcct
     9421 ggcatcctgc gtcatcagga ctcagggaag ggcggcgaat aagctgttgt gtcagctcaa
     9481 taaccgggca cgacatagac tacacctcat ggaaaaattg ctgataactg gaatcactga
     9541 aacccagcag cataggctta ccaggcgcac agagcaatgg acgtttgata attgcaggca
     9601 tttcagtcat taatgccgcc gcagaggccg catcggtgat tttattgcgg gtggtttcgt
     9661 ccagtttacg ccaggttgta ccacgggtgt tgagtaacgc ttcccagcct aattcgttga
     9721 taaaaccgtt caataattcg ctgtccagcc catcgacgcg gtaatcatga aaacgatagt
     9781 cgatgttatt ggcttccagc caacggcgag cctttttaat ggtgtcacaa tttttgatgc
     9841 cgtaaagtgt aaccataatg aatccttttt cgatttat
//
LOCUS       DAPHBJ010000164         9868 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.166, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000164 DAPHBJ010000000
VERSION     DAPHBJ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.166"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..761)
                     /locus_tag="RHZ76_003540"
     CDS             complement(<1..761)
                     /locus_tag="RHZ76_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN/FAD transporter"
                     /protein_id="HDV2814123.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLN"
     gene            766..841
                     /locus_tag="RHZ76_003541"
     tRNA            766..841
                     /locus_tag="RHZ76_003541"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:799..801,aa:Asn,seq:gtt)
     gene            complement(879..1829)
                     /gene="cbl"
                     /locus_tag="RHZ76_003542"
     CDS             complement(879..1829)
                     /gene="cbl"
                     /locus_tag="RHZ76_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HDV2814124.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(1931..2848)
                     /gene="nac"
                     /locus_tag="RHZ76_003543"
     CDS             complement(1931..2848)
                     /gene="nac"
                     /locus_tag="RHZ76_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HDV2814125.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     VSSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVMSSPVMEKRQWQLVS"
     gene            3174..3249
                     /locus_tag="RHZ76_003544"
     tRNA            3174..3249
                     /locus_tag="RHZ76_003544"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3207..3209,aa:Asn,seq:gtt)
     gene            complement(3330..4241)
                     /gene="ldtA"
                     /locus_tag="RHZ76_003545"
     CDS             complement(3330..4241)
                     /gene="ldtA"
                     /locus_tag="RHZ76_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDV2814126.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQI"
     gene            complement(4303..5382)
                     /gene="cobT"
                     /locus_tag="RHZ76_003546"
     CDS             complement(4303..5382)
                     /gene="cobT"
                     /locus_tag="RHZ76_003546"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDV2814127.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVVDVGIDSAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKQYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELATSNIVLPGNTTSDLNS"
     gene            complement(5394..6137)
                     /gene="cobS"
                     /locus_tag="RHZ76_003547"
     CDS             complement(5394..6137)
                     /gene="cobS"
                     /locus_tag="RHZ76_003547"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDV2814128.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(6134..6679)
                     /gene="cobU"
                     /locus_tag="RHZ76_003548"
     CDS             complement(6134..6679)
                     /gene="cobU"
                     /locus_tag="RHZ76_003548"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDV2814129.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWRTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            8153..8986
                     /locus_tag="RHZ76_003549"
                     /pseudo
     CDS             8153..8986
                     /locus_tag="RHZ76_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
     gene            complement(9026..9172)
                     /locus_tag="RHZ76_003550"
                     /pseudo
     CDS             complement(9026..9172)
                     /locus_tag="RHZ76_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltranspeptidase"
     gene            9256..9681
                     /locus_tag="RHZ76_003551"
     CDS             9256..9681
                     /locus_tag="RHZ76_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDV2814130.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            9678..>9868
                     /gene="tnpB"
                     /locus_tag="RHZ76_003552"
     CDS             9678..>9868
                     /gene="tnpB"
                     /locus_tag="RHZ76_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV2814131.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGL"
BASE COUNT         2645 a         2582 c         2188 g         2453 t
ORIGIN      
        1 atattcaggc taccgttaat cagtagcggt attttcgtat tccctgcacc acgaagtgcc
       61 ccgctaccaa tcaaagtgat ggcagctgct ggataactga gtaccgtcag ctccagataa
      121 gtcaacgcca gtgctttaac ttctgtcgtg gcatcacccg cgacgaaatc aataatttgt
      181 tcgccaaaat gatgaatcag cgttgccaac agtacggcaa acaacgtcat gatcaccaat
      241 gactgccgcg tcgccaccct cgctcgtcgt cgatcccgct taccgagact aaatgccaca
      301 acgacagtgg taccaagatc gatagctgca aaaaaagcca taatgaccat attgaagctg
      361 tcggccaatc ccacgccggc catcgcatct tttcccagcc agctgaccag aaaagtgctc
      421 agaacaccca tcaacaggac acaggcgttc tccatgaaga taggaacagc aagcggggtt
      481 atctcgcgcc agaacaacac tttgtagctc ttgcgtttag cgtgccagcg agtgccgtga
      541 acaacctggc gcaaagcaga ggagatattc aaagccgacc ttaattgcaa aaagtgaaac
      601 cacatttcaa ataatgaggg agaatcagca aagctgcaaa gattttcgcc aacaaattgt
      661 ctgcaaatgc aacaaactgt tgatagaaac ggcaaacagt tgggaaattt aaaaatcggg
      721 tttgacaaaa gatttttcgc cgttaagatg tgcctcaaca gcgattcctc tgtagttcag
      781 tcggtagaac ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc
      841 aaatttctga aaagcccgct tttatagcgg gctttttgct atatctgata atcaatttcc
      901 tcttcactgt tctccatcac ctggcgcttg atatcctcaa ctgacagtcc tgcattacaa
      961 agttccagaa agcgccagac ataattacgt tgaagttgcc ctcgcttcag tcccaaccat
     1021 acagtattgg catcgaaaag atgccgcgta tccagacgga ttaaattctc ttcctcttgt
     1081 tcgccgctgg attgttcggc aactaatcca atccccagcc caagagcaac ataggtttta
     1141 atgacatcag aatcctgcgc acttaataca atatctgcca gcaaaccttt gcgggcaaat
     1201 gcgtcatcaa tacgtgagcg ccccgtaatc ccctgtcggt aagtgattaa cggccacttc
     1261 gctattgatt ccagcgtcaa cggtgtaatt tgcgtcaagg gatgatcgag tggaacaagc
     1321 aaactatggt gccaacgaaa ccacgggaag gcgacgagct gcgggtcatt actcaaacgc
     1381 tcgctggcga taccaatatc agcttcgcca ttttgcaaca acgtcgcaat ttcctgtggc
     1441 gtaccctgga ttagctccag ccgaacctcc gggaaaagtt cgcgaaaagc tttaatgacc
     1501 tcaggcaagc tataacgtgc ctgagtatgc gttgttgcaa tagtgagaac gccagacgta
     1561 tcgttggtaa acaggtctgc aagccgacga acattactgg cttcattcag aatacgttct
     1621 gcaatgacca gtaatgcttt gcccggttca gtcatgccca gcagtcgctt acctcgtcga
     1681 acaaatattt cgatgccaag ttcatcctcc agttcccgaa tatgacggct gacgccggac
     1741 tgtgaggtaa aaagcatatt cgcaacctct gtcaggttgt aatcctgacg tgcagcctcg
     1801 cggattatct ttagttgttg gaaattcacg gtaaactccg ggcagttcag attttccgtt
     1861 attgttaaag tctaatgccc ggcataacaa ataataaaaa cccgcatctt attccatccc
     1921 gatataacgc ttagctcacc aattgccact gccttttttc catcactgga gaactcatca
     1981 ctgacattaa caactctttc actgcctgtg cctgtggtga taagttcgct ctggcgggta
     2041 aatttaatga caaagagaga ctcatggaag gtgtggtaat gcgtgacatc cacccgttta
     2101 ctgcgccaca taacgaacgc gcggccgatt cgggtaatac tgcaacgccc atgccgctgg
     2161 caatcgctgc ggtaagcgtg gcaatagact caatttcacc aatgactttt gccgtgagtc
     2221 gccgtaggga aaaagcctca tcaacacgaa gtctaacagc actgtaatca ctgggaagaa
     2281 agaggttcat ttgcgcaata gcattaacat caacgctttg ccccggacaa tcttgagtcc
     2341 ctaccaggaa aagatcttct ttcagtaaag cctgactgga tacaccagcc acaggggaat
     2401 gctcataaat caccgccata tcgagttggt gatttatcag tttttcgtta agcactgcac
     2461 cactattttc atgaagatag ataacgacct ccgggaattc agcgcgaacc gcctgtaata
     2521 agggcatggt gatggatgac acagcggttc ctggcgcaaa gccaatcgag acttgccccg
     2581 ataatgactg accaacgtta tgcaccgcca gttgggcctg ctcacactga cgtaaaatgg
     2641 cccgtgcatg ggtatagaga atttttccgg cgtctgttgg cgtaacgccc cgttttgtgc
     2701 gaatcaaaag ttgttgattt aactcacctt ccagtgtggc aacctgctgg ctgagcgctg
     2761 gttgtgcgat atgcaatact tcagcagcct gagtcaggct accaatatct acaattttta
     2821 cgaagtattt caggcgtctg aagttcatgt tgcctccggt ttttgagaat cggcccaagt
     2881 gccgccatta cttacaacca gactgcaaga tgcttgccag ttttattttg gcgttgatgt
     2941 acaagctaac caactgtcaa ataagagatt atgatagatt cgtcattggc tcttttcacc
     3001 agcagtcgcg ttcccctgcc ctataaaagg agggtatgca ccacgatggt tcattaccaa
     3061 taagattgaa atctcatcac tttgttgaaa ttggcagcaa acaaacaaaa aaatgcattt
     3121 caccctttga catcaccatg cactgccatt aatatgcgcc ccgttcacac gattcctctg
     3181 tagttcagtc ggtagaacgg cggactgtta atccgtatgt cactggttcg agtccagtca
     3241 gaggagccaa attcaaaaaa gcctgctttc tagcaggctt tttgctttct aattaccaac
     3301 gctcttaaaa catctgtctt gaaccagaac tatatttgca caggcattcc cgatcgacgt
     3361 tgcaacgcag catttgcgcg attcacatca acttcttgcc cgttgataaa cgcccgcaaa
     3421 gatggggtta ccggcaatgg cacttttcgg tcagactcat attctgcacg attgcgcgac
     3481 agcggctcat gaacttccag ccagttcgag ccatctggtt cagtggtgta ttttactggc
     3541 tggtcgataa tttgcacacg cgttccaaca ggaacattat caaacagata tttgatatcg
     3601 tcattgcgca gacgaataca gccctgactt acccgaagtc caataccaaa attggcattg
     3661 gtaccgtgga tggcatacaa cctgccaata taaatcgcgt acagccccat gggattatcg
     3721 ggccccgcag gaacaaatgc gggcaaactc tcccctcgtt tcgcatattc gcgccgagtg
     3781 ttcggcgttg gcgtccaggt tggagcttct tgtttacgtt caacggtagt cacccagtta
     3841 cgcggggttt ctcgcccagc ctggccgata ccaataggaa agacttccac agtattactg
     3901 tctggcgggt agtaataaag acgcatctca gcgacgttaa ccacaatccc tttacgaaca
     3961 gtggcgggca aaatcagttg ctgcggaatg gtgagttgcg agccagactt cggcaagaaa
     4021 acatcagcgc ccggattcgc ttccagcatg ttacttaacc cttgcccgta ttgtgcggca
     4081 aaagtctcca gcggctgggt attgtgatca ggaacagtta cagtaagcga ctgccccacc
     4141 aaacggctac cctctggagg taatggataa gttaccgcca ggctagtatg gctggcaaaa
     4201 agcagagcaa atgagcaaag aatatttaca cgacgcatca tgtccctttc ctatgtcgcg
     4261 aaagctatcc gttaagtata gcttttatca gacttttcgt ttttaactgt tcaaatcaga
     4321 agtcgtattc cccggtagaa caatattact ggtagcaagt tcgcccatgt tgttatatat
     4381 cgcacaggca gcttcgatga tgggcatcgc cagagctgcg ccactcccct cacctaaacg
     4441 catctccata ttgagataag gctccagccc caaatgcgac agcgctatac gcgccccttt
     4501 ttctgccgac aggtgagaag gaatgagata ctgtttgatt gcaggagaca tctggcaggc
     4561 tgcgagcgca gcagcataag aaagaaatcc atccagcaac acgggtaaac cgcaggaagc
     4621 agcgcctaac atcacgccag ccattccgac caaatcaaat ccccccactt ttgccagaac
     4681 atcaacgcca tcctgcggat ttggttgatt caacgtaatc gcccggcgca caacatcaat
     4741 tttattagcc agtttatctg tcggcaggtt tgcgccaatc ccaaccactt cttcaggatc
     4801 ccggccagtg attgtgctga ctattgccgc tgccggtgtc gtgtttgcca tccccagttc
     4861 acctacacca aacagcgtga caccgttttt tgccagctcc cgcgtataac atatgacgtc
     4921 caaaagcaac ttttcagcct gacggcgact cattgccgga gctgaagcaa tattgccgct
     4981 acctcgtgcg acacgcatgt tgataagccc ggggataggc tcagcactat caataccaac
     5041 atcaactacg tggacgttag cgcccgcttg tgctgccagc acacacacgc cggttgttcc
     5101 gcgggtcata ttttcagcct gaatggctgt cacttctttt ggggaaatag cgaccccttc
     5161 ctcccagacg ccgtgatcgg cacacataac cagtaccgct tttttgccca catgcggtat
     5221 gccattcaac cccggcattc ccgccagttg tatggcaagc gcctccagcc ttcccaggct
     5281 accaacaggt ttgagtaacc cgtcaatatg ccgttgtgca cgcgacatag cggcaggatc
     5341 gatagcaggg atcgtattca gtaaatcggc aagtgtttgc atgtcacgtc tctttatagc
     5401 agtgccagca ggaagaccag ttcaccaagc tctatagcag ctcccagcgt atcgccggtt
     5461 tgcccgccca gcgtacgttt gagaagctga ccgaggatga aaatcgccac catcgtcacc
     5521 accatagcag ccacaccatg catacccggc aataatacgg cagcaaaaat agcggctaag
     5581 ccgagtgtga cgcaggtttg tcgcccatca attttgccaa tgaacacatt gccaagccct
     5641 tcttcccggg cataacgatg acgatacatc aataatgcgg cagtcccgcg gctgaccgca
     5701 catgctgccg ccagcgatgc aaggatcggt tcgccacgca gagccaactc gcttaatacc
     5761 agaatctttg ccagtacaac aaaaatcaat gccagaccgc catgggtgcc taaacggcta
     5821 tcacgcatga tttccaacat gcgatcgcgg ctacgtgcag aaaatacgcc atcacaggta
     5881 tcggcgaggc cgtctaggtg gaatcccccg gtcatcagcg ccagcacaag tacgctaaac
     5941 agtgccgcca gtggtacgcc acaccatgcc tgcagcacca tgaagaccag cccgctaatc
     6001 gcgccaagca ataatccaat taaaggaaaa gtaataatac cgcgagaata atgctcgaaa
     6061 tccagtccct gggaccagcg acgcggtaca ggcagacgcg taataaatga gagcatcgcc
     6121 caaaataatt tactcatttg atttttactc caatacccga aaccaccagc catacttcat
     6181 ttgccgcagc ggccagctgc tgatttaccc gcccggcaat atcacgaaaa tgtcgtgcca
     6241 gacgactctc cggcacaatc cccattccca cttcgttagt cactaataca acctttgcgg
     6301 ggcaacgttg gcaggcagca atcaacgact gaatctcagc attaatcgcc tgttccatcg
     6361 cctgataatc ccattcatca gggtctttat cgccgccata atcaaacaac agattagtca
     6421 ccattgtggt aacgcattcc agcaacacag cttcatgcgg gttaatgtct gcatgaatta
     6481 attcatcaag atgttgccaa cgctccaccg tgcgccagtg cgccggacgg ccttgccgat
     6541 gatgttctat ccgtgcagcc atctcgtcat caaggatttg cgaggtggcg atatacagaa
     6601 cctgtgaaga gtccccaata agcgcctctg cgtggcgact cttcccgctc cgtgcgcccc
     6661 ccgtcaccaa aatcatcata ccgactcctg attcaattgt atgatggtat tcattgaaca
     6721 gaacaatgga tcccccacca ttgctgaccg caaaacattt acacgatcaa tactgcgcgt
     6781 aatataatta aaaatccagc attctcaatc cattacgaaa gaagatggca ttgtggaata
     6841 gccagacttc ctcgtgttaa cgtccgacaa aatgcgtcat tgacagcggt ttctgtggat
     6901 tgacacattt cattcagata atgaattaat gctactgccg gaccaaccag caaacgggca
     6961 ttgtgttctg aaatccaggc tattgattca acgtcagcga cagatctgcg ctgtaaaaga
     7021 acttgtaaaa caacgcaata gaattatccg gaatggtggc gactatgcac tagaacacca
     7081 caatttcgct ctctcggtaa ggcactgtca cagcattcaa cagaatgtga cttgccagat
     7141 ttattagcgc caccagatgt ttaaccgggt aaccacgacc cagtcgggga tatattccat
     7201 cttctcgctg acatcatcaa gaataaaaag gtttatcaca ctcagagcaa cgttcttcag
     7261 caggcgggat aattcttcaa catttaaaag atgtgttatt aaaggcatac aaactggacg
     7321 tattgtattt tcttttggtg gttgacctaa ggtagcagtt tatcctgatg cgctgagatt
     7381 tctgtaatat ctacgtcaac attctcctcg aatagaaaat gcagcctcgg caagtgattc
     7441 acatttttta acaaaacgtt gttagcgtgc cattttcaga agctctccaa gaaagcgaat
     7501 tcttttctca aattctgcat tctcagacac agctttctac tccatggcaa tggcccaaat
     7561 acgaagttgc tcaggatcat tagtggtgct gagaagttaa ataaccatgc ggagtaccac
     7621 tcgtcatcac gtatttatat atcatgaagc tattgttgtg ttttgtacat cagtagatgt
     7681 attctgatat actccttttg ctagacataa cctttcacct gcttgcaaag cttctgtgtt
     7741 ctgacattgc caaattgttg caattctgta tccagccttc tttcagtcat agcttcgggc
     7801 cgcgataaga ctcactgatc tgaccctgat tcctcttgca gactttatag accaattaaa
     7861 atgcagtttc tgcaggtcaa cgtctgacca tcattgtcat cactctgccc attagagtaa
     7921 ccttctgcat tcatcctttt gtaaaaagtt tatgtcagta tcagcaatta accggacctg
     7981 atactgatat gagtcttacc gcatatacgg tcaatttcag caattaatta cattatccac
     8041 gccaaagtgt ttgtcatcgc aatgatggta ccttctttca gacaccattt tttcaactcc
     8101 gttttccacg gaccgcactc ttatgtcaag agtgcggtga tcctacccac gtaatatgga
     8161 ccgcaaacct attgaagatg tgatcttcga aattaataac tttatctcgc tcggtggtcg
     8221 caccattgtt gatgccacag ggtctgagtc tatcggacga gatgcgcagg cattacgaga
     8281 agtcgcatta aaaacaggtc tgaatattgt tgcttcctcc ggtccctacc tggaaaaatt
     8341 tgaaagccag agaattcata aaacggttga tgaactggcg gcaacgattg ataaagaatt
     8401 gaatcaaggg attggcgata cggatattcg tgccggaatg atcggtgaaa taggtgtctc
     8461 accgacattt accgaagccg agcataacag cttgcgggct gcctcgctgg cacagattaa
     8521 caatcctcat gtggcgatga atattcacat gccgggctgg cttcgtcgcg gtgatgaagt
     8581 actcgacatt gtgttaggtg aaatgggcgt ctcgccaaat aaagtctctc tcgcacactc
     8641 ggatccgtca ggaaaagacg tggcgtatca gcggaaaatg cttgataagg gtgtctggct
     8701 ggaattcgac atgattggcc tagacattac cttcccgaaa gagggaatag cgccaggggt
     8761 gcaggagact gccgatgccg tcgctcatct cattgagttg ggatacgccg atcagcttgt
     8821 tctcagccac gatgtcttcc ttaaacaaat gtgggctaaa aatggcggta atggctgggg
     8881 atttgttcca gatgtttttc tggcctatct ggcggagcgc ggcgtcgata aaacgatcct
     8941 caaaaaactc tgtatcgata atcccggacg attattgacc gcataaaacc attatccccc
     9001 ggcacactgc gccgggggat gtcaattaat accccgccgt taaatcatcc accaagcgcg
     9061 agtcggatgc gccgtacaac tcaccatccg acccaaccat aatgctttgc gtgctgccca
     9121 tcgcctcatt cagcgccact ttctgacctt ttgcttccag cagcttgagc gtaagcgtaa
     9181 actgaccgcc gtatgtagcc atcagacgag aattggtaac ttagacgccc atctgatata
     9241 gacggacatc taagtatgga attacaggac tggcgaaaag aacctcgtaa aaactattcg
     9301 aatgaattca aacttcgtat ggtggaactg gcatcacaac ctggagcttg tgttgcacag
     9361 attgcacgtg aaaatggcgt caatgataat gttattttca aatggctcag gctctggcag
     9421 aacgaagggc gtgtttcgcg gcgtcttccg gtaacgacct cttctgacac tggcgttgaa
     9481 ttattacctg tagaaataac gccggatgag cagaaagaac ctgtggcggc cattgcgccg
     9541 tctttatcca cttccactca gaccagagtc agtgccagtt cctgcaaggt ggaattccgt
     9601 cacggtaaca tgacgctgga aaatccatcg ccagagctgc tcacagtgtt gatccgtgaa
     9661 ctgaccggga ggggaagatg atctcactcc catcaggtac ccgtatctgg ctcgttgccg
     9721 gcgttaccga tatgcgtaaa tccttcaacg gactgggaga acaggtacaa catgtgctga
     9781 atgataatcc cttctccggt cacctgttta tcttccgtgg ccgacggggt gacaccgtca
     9841 aaattctttg ggctgatgct gatggtct
//
LOCUS       DAPHBJ010000165         9849 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.167, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000165 DAPHBJ010000000
VERSION     DAPHBJ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.167"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..322)
                     /gene="yfdX2"
                     /locus_tag="RHZ76_003553"
     CDS             complement(<1..322)
                     /gene="yfdX2"
                     /locus_tag="RHZ76_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat resistance protein YfdX2"
                     /protein_id="HDV2814132.1"
                     /translation="MNEQTPNPNATNEGINEQAAVSSLPVSPESKPEVVTEVQPEVQK
                     ETDSQAADKRKQVLDEAVSALSLTKFALAALDGKDTARALATLAEVTGKLELIVAREP
                     TLALA"
     gene            complement(425..1333)
                     /gene="yfdX1"
                     /locus_tag="RHZ76_003554"
     CDS             complement(425..1333)
                     /gene="yfdX1"
                     /locus_tag="RHZ76_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat resistance protein YfdX1"
                     /protein_id="HDV2814133.1"
                     /translation="MNIKQPQYTFSALALAVVVGLSGPALAQSAASPSAAAPSAASKA
                     AQPQVDDKAAREADKKRAELTQDAITALTKTQEALTLLDAKKTKEALAALELASGKLE
                     LVLARDAKLALAPVDVRVITHDIHANVESVKKAVKLSRELLGDGEVQKARPIVANLAS
                     EIVIQTDNLPMATYPAAIKSAVRLIDSGKIDNAKAELARALNTLVVTSVAFPLPVLRA
                     EAAMAKAEKLAETDRRDAKQNEELSTLLSSVRTEIEMAQILGYGKKADFKPIFDQVKS
                     IEQKSAGGKSGKGWFDELKTRIQKLF"
     gene            complement(1356..1814)
                     /gene="hsp20-GI"
                     /locus_tag="RHZ76_003555"
     CDS             complement(1356..1814)
                     /gene="hsp20-GI"
                     /locus_tag="RHZ76_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock protein sHSP20-GI"
                     /protein_id="HDV2814134.1"
                     /translation="MSALTPWDPFRELDELQNRLATMFGRIPQRQGARTGNEAMTTAD
                     WAPMADISEDENAFLLKLDLPEVPKDAVRVSAENGVLTISGERKLEKEEQGKKFHRIE
                     RAYGRFVRSFVLPDNVDPTKVTASMKDGVLEVRLVKAEQAKPKQIEISVN"
     gene            complement(1902..3618)
                     /locus_tag="RHZ76_003556"
                     /pseudo
     CDS             complement(1902..3618)
                     /locus_tag="RHZ76_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761381.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
     gene            complement(3679..3870)
                     /locus_tag="RHZ76_003557"
     CDS             complement(3679..3870)
                     /locus_tag="RHZ76_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814135.1"
                     /translation="MAGEAAVSDPIGLAWAASLASIAVAVVAAGHAVVYKRDPRSATP
                     WGLLISGGTALPLAGHPFA"
     gene            complement(3870..6719)
                     /gene="clpK"
                     /locus_tag="RHZ76_003558"
     CDS             complement(3870..6719)
                     /gene="clpK"
                     /locus_tag="RHZ76_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock survival AAA family ATPase ClpK"
                     /protein_id="HDV2814136.1"
                     /translation="MARKQCQVCSQPATVRVEANLNGRHSTMLLCDDHYRQLVRQQKR
                     TVSPLEALFGSRSGLFEDFLGSDFFRIGDDATPVAADTDDVVDASFGEPADAGSGVPR
                     RRGSGLASRISEQSEALLQEAAKHAAEFGRSEVDTEHLLLALADSDVVKTILGQFKIK
                     VDDLKRQIESEAKRGDKPFEGEIGVSPRVKDALSRAFVASNELGHSYVGPEHFLIGLA
                     EEGEGLAANLLRRYGLTPQALRQQVSKVVGKGAEDGRAETPTNTPELDKYSRDLTKMA
                     RDGKLDPVIGRAQEIETTIEVLARRKKNNPVLIGEPGVGKTAIVEGLAQRMVAGEVPE
                     TLRDKRLVELNINAMVAGAKYRGEFEERVQKVLKEVTEHQGELILFIDEVHTIVGAGQ
                     GGGEGGLDVANVFKPMMARGELNLIGATTLNEYQKYIEKDAALERRFQPVTVPEPTVA
                     QTIMILRGLRDTFEAHHKVSISEDAIIAAAELSDRYITARFLPDKAIDLLDQAAARVK
                     LSATARPVAVQELESELHQLRREQDYVAARKQYDQAAELGKRIEAKEAELKKLVENWE
                     RERASGSAEVKAEHVAQIVSRLTGIPVNELTVEEREKLLHLEQRLHERLVGQDEAVRA
                     VADAVRLSRAGLREGSKPVATFLFLGPTGVGKTELAKALAESIYGDEHALLRIDMSEY
                     GERHTVARLVGAPPGYVGYDEGGQLTEKVRRKPYSVLLLDEIEKAHPDVYNILLQVFD
                     DGRLTDGKGRVVDFTNTIIIATSNLGSDIIQRRLKARGAADEEYEKTKAEVMDVLRGH
                     FRPEFLNRIDEIIVFHALGKEEIRHIVGLQLDRVARSAASQGVTLTFDQTLIDHFAEE
                     GYKPEFGARELKRLIRSELETALAREMLGGGIGKGDHASARWDDKAERVVFERKEPPQ
                     TPAEPEQPDAAKATETPHGDAGKGSRKKKSASDAS"
     gene            complement(6825..7394)
                     /gene="hsp20"
                     /locus_tag="RHZ76_003559"
     CDS             complement(6825..7394)
                     /gene="hsp20"
                     /locus_tag="RHZ76_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003847761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock protein sHSP20"
                     /protein_id="HDV2814137.1"
                     /translation="MDIDFKKLAPWNWFKNEQQEQQTASSLPVQRNDLPAASGPVSPL
                     LQLHREIDRLFDDAFRGFGFPALNMPQWPSDWSGMLKPALDIQETDKQYKIALEVPGV
                     EEKDIQITLDNDVLMVRGEKRQEQEKKEGGFHRVERSYGSFQRALNLPDDANQDSIKA
                     SFKNGVLTVTIDKREVSAPKQGRSIPING"
     gene            complement(7429..7710)
                     /locus_tag="RHZ76_003560"
     CDS             complement(7429..7710)
                     /locus_tag="RHZ76_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001568597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV2814138.1"
                     /translation="MKAHLQVIFTLDELAAYLKVGKRTLYRLAAHGEIPAFKVGGTWR
                     LRQSEIDQCINDQTRAGAKKEVMRKREQQKSSEQPVSPGRTARRSRQRA"
     gene            complement(7932..8195)
                     /locus_tag="RHZ76_003561"
     CDS             complement(7932..8195)
                     /locus_tag="RHZ76_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814139.1"
                     /translation="MGHGLRRRCREGVLAGRILLNYVVWGNGSVSARLWNAIRSDDWA
                     IPHVGLSSLGEIVVWARPDEFPPRNMQTSKGLRALGYNVRIGV"
     gene            8210..8473
                     /locus_tag="RHZ76_003562"
     CDS             8210..8473
                     /locus_tag="RHZ76_003562"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2814140.1"
                     /translation="MRPQRRTYSKSFKAQVIQECSEPGASIANIALGYSLNANLVHKW
                     IRQLTQKTTAIQPAFIPLPSQMLGAGSHAQARCCDHEFDPVGP"
     gene            8418..8582
                     /locus_tag="RHZ76_003563"
                     /pseudo
     CDS             8418..8582
                     /locus_tag="RHZ76_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010955146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            8800..9648
                     /locus_tag="RHZ76_003564"
                     /pseudo
     CDS             8800..9648
                     /locus_tag="RHZ76_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            9716..>9849
                     /locus_tag="RHZ76_003565"
     CDS             9716..>9849
                     /locus_tag="RHZ76_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDV2814141.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     "
BASE COUNT         1796 a         3107 c         2860 g         2086 t
ORIGIN      
        1 cggccagggc gagcgtgggt tcgcgcgcga cgatcagctc cagctttccc gtcacttcgg
       61 ccagcgttgc caatgcgcgt gcagtgtcct tgccgtcaag tgcggccagc gcgaatttgg
      121 tcagcgacaa ggccgagact gcctcatcga ggacttgttt acgtttgtct gccgcctgcg
      181 agtccgtttc tttctgaacc tcaggctgta cctccgtgac gacttcaggc ttggactctg
      241 gactgacagg aaggctgctt accgcggcct gttcgtttat tccttcgttc gtcgcattgg
      301 gattcggtgt ttgctcattc atggtgatat gcctcgtatg agatagtgaa aataggcgaa
      361 tctattgggg ctgaatgcga acatcgcgag actgaccgac agagcagtca gccccttcat
      421 cacctcaaaa cagcttttgg atgcgcgtct tcaactcgtc gaaccatccc ttgccgcttt
      481 tgccaccagc cgacttttgc tcaatggact tcacctgatc gaagatgggt ttgaagtccg
      541 ccttcttgcc ataacccagg atctgcgcca tctcgatctc cgtgcgcacg gacgacagca
      601 aggtgctgag ctcctcgttc tgcttggcat cgcgcctgtc ggtctcggcc agcttttcag
      661 cttttgccat cgcggcttcg gcccgtagca cgggcagagg aaaggcgacc gaggtcacca
      721 ccagcgtgtt cagtgctcgg gcgagttccg ctttggcgtt gtcgatcttg ccgctgtcga
      781 tgagccgtac ggccgacttg atcgctgccg ggtacgttgc catcggaagg ttgtcggttt
      841 ggattacgat ttcgctggcc agattggcaa cgatgggccg ggccttttgc acctcgccat
      901 cacccaacaa ctcccgagac aacttgaccg ctttctttac cgattctacg ttggcgtgga
      961 tatcgtgggt gatgacgcgt acatcgaccg gcgccaaagc aagtttggcg tcgcgtgcca
     1021 ataccagttc cagctttccg ctggccagtt ccagcgcagc aagcgcctcc ttggtcttct
     1081 ttgcatcaag gagggtcaaa gcctcctggg tcttggtgag cgccgtgatg gcgtcttgag
     1141 tgagctcggc acgcttttta tcggcttccc gggcggcctt gtcatctacc tgtggctgtg
     1201 ccgccttgga tgcggcagag ggtgctgcag cgcttggact tgccgccgat tgggccagag
     1261 ccggtccgct cagtccgacg acgaccgcca gggcaagtgc ggagaaggtg tattgtggct
     1321 gtttgatatt catgatcttg ggctcctgat tgaagttagt tgactgagat ttcaatctgt
     1381 ttcggtttgg cttgctcggc cttgacaagc cgcacttcca gtacgccgtc tttcatggaa
     1441 gccgtcacct tggtcggatc aacgttgtca ggcaagacaa agctgcgcac aaagcggcca
     1501 tacgcacgtt cgatgcggtg gaacttcttg ccctgctcct ctttttccag tttgcgctcg
     1561 ccgctgatgg tgagcacacc gttttccgcg ctgacgcgca cggcatcctt ggggacctcc
     1621 ggcagatcca gcttgaggag gaatgcgttc tcatcctcgc tgatgtccgc cattggtgcc
     1681 cagtccgccg tggtcatggc ttcgttgccg gtacgggcgc cctgtcgctg gggtattcgt
     1741 ccgaacatcg tcgccaggcg gttttgcaat tcatccagtt cccggaaggg gtcccacgga
     1801 gtcaatgcag acatatcggt tctccttgaa caatgccgac gaattggatg acgcactgaa
     1861 cagagccgtc ggtttactct gaatggcacc tactactgtt ttcagttccg aacaagtcct
     1921 tgagtcaatt gacggatatc gctttcgtcg agcgtttccc gcgccagcag ctcgcacgca
     1981 cagcgctcca gcaccgcgcg gttgatgtcg agaatccggt aggcgtgctc gaacacgccc
     2041 atcacgatgt cgcggatagc ctgatcgatg cgcgcctggg tcgattcggc cacccggcaa
     2101 ccgccgtggg ccagttccgg tgtatcgaga aagcggggcc gctgcgcctc gaaggcgatg
     2161 tagcccaggc cctcgtccat gccaaagcgg gtgatcatgt cgcgggcgat gtcggtggct
     2221 cgcgccagat cgtccgccgc cccggtagac aactcgccga acaccagctt ttcggcggca
     2281 cgcccgccca gcagtacggc gatcttgtgc tcgagatcgg tacgcgtcat caggaagcgg
     2341 tcttcggtgg ggggctgcaa ggtgtagccc agcgcgccga tgccgcgcgg gatgatcgag
     2401 atcttgtgta cggggtcggt ttcgggcagc accagcgcat ggcccatctc gtgatgggcc
     2461 acggtttccc gctccttggg attgagcacg cggctcttct tttccaggcc cgccacgatg
     2521 cgctcgatgg tcgcggtgaa gtcctgcaat tccacggcgg acgcacggcg tctcctcgcc
     2581 gccagcgccg cctcgttgac cagattggcg aggtccgcgc cggaaagccc gtggtcagtg
     2641 cggctacctg ttcgaggtct acatcctggg ccagcgtcac cttcttgaca tgaaccttca
     2701 ggatgtccag ccgccccttc ttgtcgggcc ggtccaccag ctcctggcgg tcgaagcggc
     2761 cggcacgcag cagcgcctgg tcgaggattt cggggcggtt ggtggcggcg aggatgatca
     2821 gcccgaccga gctgtcgaag ccgtccatct ccgtgagcag ctggttgagg gtctgctcgc
     2881 gctcgtcgtg gccgccgatg ccgcgcacac gggccgcacc cacgccgacg aacatctcga
     2941 caaactctga tccggagatg gagaagaagg gcaccaccac ctcgccggca acagccttgg
     3001 ccagcaatgt cttgccggtg cccggtgggc cgaccagcaa cacgcccttg ggaatgcgcg
     3061 ccccgagacg gccatagtcc tgcggattct tcaggaaatc gacgatctcg accagctccg
     3121 ccttggcctc atcccccccg ccacatcggc gaacgtcaca ccggtgttct tttccacgaa
     3181 caccttggcg cggctcttac cgatgttgag aaacccgccc atgccctgtt tctcggcgaa
     3241 gcggcggaac aggaagaacc agacgccaaa gaaggctacc gctggcagga tccacgacag
     3301 cacatcgcgc agccaggtgc tctccaacac gcgggcatag ggcacgtcgt acttcgacag
     3361 gcgatcggca agatcgggtt cgaccctcgt tgccacgatc gtcgtcttgc cccggctgtc
     3421 cggcgacttc aggcgcccgg tcaccgtacg atccgacacc agcacttcgg ccacgcgcac
     3481 ctcggccagc gccttctcga actcgctgta gggcaccggc tcgacggttt tcgccgtctg
     3541 ccagtagctc tgcaaactca gcaacagcag gaaggcaacg atccagtagc cggtattcca
     3601 ttgattcttt ttttccatgg gccttgttcc tcgaaagtcg agttgccaga ggatcaggtg
     3661 aacagacggg ccgcaccgtc acgcaaacgg atggccagcg agcgggagag cagtgccgcc
     3721 gctgatgagc agcccccacg gcgtggccga ccgcggatca cgcttgtaga cgaccgcgtg
     3781 acccgcggcc acgaccgcca ccgcgatcga ggccagggag gctgcccatg ccaggccgat
     3841 ggggtcggac acggcagcct cgcccgccat caagatgcgt cgctcgccga cttcttcttg
     3901 cgcgagcctt tgccagcgtc gccgtgcggc gtctcggtcg ccttcgcggc atccggctgc
     3961 tccggctcgg ccggggtctg cggtggctct ttgcgttcga acaccacgcg ttcggccttg
     4021 tcgtcccagc gtgcgctggc gtgatcgccc ttgccgatgc cgccaccgag catctcgcgc
     4081 gccagcgcag tttccagctc gctgcggatc aggcgcttga gttcacgcgc accgaactcg
     4141 ggtttgtagc cttcttccgc gaaatggtcg atcagcgtct gatcgaaagt gagcgtcacg
     4201 ccctggctgg cggcgctgcg cgccacgcga tcgagctgca ggccgacgat gtggcggatc
     4261 tcctccttgc ccagcgcatg gaagacgatg atctcgtcga tgcggttgag gaactcgggc
     4321 cggaagtggc cgcgcagcac gtccatgacc tcggccttgg tcttttcgta ctcctcgtcg
     4381 gccgccccac gcgccttcag ccgtcgctgg atgatgtccg aacccaggtt ggacgtggcg
     4441 atgatgatgg tgttggtgaa atccaccacc cggcccttgc cgtcggtgag gcgaccgtcg
     4501 tcgaacactt gcagcaggat gttgtagacg tcagggtgtg ccttctcgat ctcgtcgagc
     4561 agcagcacgc tgtagggctt gcgccgcacc ttctcggtga gctgaccgcc ttcgtcgtaa
     4621 ccgacatagc ccggaggcgc gcccaccagc cgcgccacgg tatggcgttc gccatattcc
     4681 gacatgtcga tgcgcaacag ggcgtgctca tcgccgtaga tcgattcggc caatgccttg
     4741 gcgagctcgg tcttgcccac cccggtcggg cccagaaaca ggaaggtagc caccggtttg
     4801 ctgccttcgc gcaggccggc gcgggacagc cgcacggcat cggccacggc acggaccgcc
     4861 tcgtcttgtc ccaccaggcg ctcgtgcagc cgttgttcca agtgcagcag cttttcgcgc
     4921 tcttccaccg tcaactcgtt gaccgggatg ccggtcaggc gcgagacgat ctgcgccacg
     4981 tgttctgcct tgacctctgc actgccggag gcccgctccc gctcccagtt ctcgacgagc
     5041 ttcttgagct cggcctcctt ggcttcgatg cgcttgccga gctcggcggc ctggtcgtac
     5101 tgcttgcgcg cggccacgta atcctgttca cgccgcagct ggtgcagttc ggactccagc
     5161 tcctgcacgg ccaccggccg ggccgtggcc gacagcttca cgcgcgcggc cgcctggtcg
     5221 agcaggtcga tcgccttgtc cggcaggaag cgcgccgtga tgtagcggtc ggacaactcg
     5281 gccgccgcga tgatcgcgtc ctcggagatg ctgaccttgt ggtgcgcctc gaaggtgtcg
     5341 cgcaggccgc gcagaatcat gatggtctgg gccaccgtcg gctcgggcac cgtcaccggc
     5401 tggaagcgcc gctccagtgc ggcatccttc tcgatgtatt tctggtactc gttcagcgtc
     5461 gtggcgccga tcaggttgag ttcaccgcgc gccatcatcg gtttgaacac gttggccacg
     5521 tccagcccgc cttcgccacc gccctggccg gcaccgacga tggtgtgcac ctcgtcgatg
     5581 aacaaaatca gctcgccctg gtgctcggtc acctccttca gcaccttctg cacgcgctcc
     5641 tcgaactcgc cgcgatattt ggcgccggcc accatggcgt tgatgttgag ttccaccagg
     5701 cgcttgtcgc gcaaggtctc gggcacttca cccgccacca tgcgctgcgc cagcccttcg
     5761 acgatggcgg tcttgcccac acccggctcg ccgatcagca ccgggttgtt cttcttgcgc
     5821 cgggccagca cctcgatggt ggtttcgatc tcctgcgcgc ggccgatcac cgggtcgagc
     5881 ttgccgtcgc gcgccatctt ggtcaggtcg cgcgagtact tgtcgagttc tggcgtgttg
     5941 gtcggcgtct cagcgcggcc atcctcggcg cccttgccga ccaccttgct gacctgttgg
     6001 cgcagcgcct gcggcgtcag gccgtagcgg cgcagcaggt tggcggccag cccttcgcct
     6061 tcctcggcca gaccgatcag gaaatgctct ggaccgacat aagaatggcc gagttcattg
     6121 gaggctacga aggcgcggct gagcgcatcc ttcacgcgcg gcgacacgcc gatctcgccc
     6181 tcgaagggct tgtccccgcg cttggcctca gactcgatct gccgcttgag gtcatcgacc
     6241 ttgatcttga actgacccag gatggtcttg accacgtcgc tgtcggcgag cgccagcagc
     6301 agatgttcgg tatccacctc ggagcggcca aattcggcag cgtgtttggc ggcctcctgc
     6361 aacaaggctt ccgactgttc gctgatgcgg ctggccagcc cactgccgcg acggcgcggc
     6421 acacccgaac ctgcgtcggc gggctcgcca aacgaggcat cgaccacgtc atcggtatcg
     6481 gcggcaactg gcgtcgcgtc gtcgccgatg cggaagaagt cactgccgag gaagtcctcg
     6541 aacaggccgc tgcgcgaacc gaacaaggct tccagcggcg aaacggtgcg cttttgctgg
     6601 cgcactaatt gccggtaatg gtcgtcgcac aacagcatgg tgctgtgacg accgttgaga
     6661 ttggcttcca cccgcacggt ggcgggttgg ctgcagacct ggcattgttt tctggccatg
     6721 ctgatgctcc ttagaaggtt gaaaaggttg aggcgacgag gcatcgcgag ccgcgacacc
     6781 tcgtctcggg ccgggttttt cttgtgacga acgagccggc gacgtcagcc gttgatcggg
     6841 atcgagcgtc cctgcttcgg cgcgctgacc tcgcgcttgt cgatcgtgac cgtgagcacc
     6901 ccgttcttga acgatgcctt gatcgaatcc tggttggcgt cgtcaggcag gttcaaggca
     6961 cgctgaaagc tgccgtagga gcgctccaca cggtggaagc caccttcctt cttctcctgt
     7021 tcctggcgct tctcgccacg caccatcagc acgtcgttgt cgagggtgat ctggatgtcc
     7081 ttctcctcga caccgggcac ttccagggca atcttgtact gcttgtcggt ttcctggatg
     7141 tccagggccg gcttcagcat gcccgaccaa tcggatggcc actgcggcat gttcaacgcc
     7201 ggaaaaccga agccccggaa tgcgtcatcg aacagccggt cgatttcccg atgcagttgc
     7261 aggagcgggc tgactggccc gctcgccgct ggcaggtcat tgcgctgcac cggcagggaa
     7321 gaggcggtct gctgctcttg ctgctcgttc ttgaaccagt tccagggagc caacttcttg
     7381 aaatcgatgt ccatgtcata cctccagaaa aattggaaaa cgagttactc aagccctttg
     7441 cctgctgcga cgggcagtgc gcccaggcga cacgggctgc tcggatgact tctgctgttc
     7501 acgcttgcgc atcacctcct tctttgctcc ggctcgggtc tgatcattga tgcactgatc
     7561 gatttcactt tgacgaagcc gccacgtccc gcccaccttg aaagccggaa tttccccgtg
     7621 tgcggcgagc cgataaagcg tccgcttgcc gactttcaag tacgcagcca attcatcgag
     7681 cgtgaaaatc acctgtaggt gcgctttcat ctcaccctct ccaatcgcgt cgctttgcgg
     7741 ttccggccaa ggaaatcctt gcaatacttt gtaatactag acctgctatg aaaaacttca
     7801 agagtcaatg tgcagcgttc tcccgcccca ttgatgcagg tcaactgctc aggcaaggca
     7861 caggggggcg gcctgatcag gcaatggaca gcgcctggct cctcagcctg cgtgtgccac
     7921 aggcgcctcg atcaaacacc gatcctcacg ttgtagccga gtgcccgcag ccccttgctt
     7981 gtctgcatgt tccttggcgg aaactcgtct gggcgcgccc agacgacgat ttccccgagg
     8041 ctgctcaggc caacgtgcgg aattgcccag tcgtcgctgc ggatcgcgtt ccagagtcgg
     8101 gccgaaacac ttccgttgcc ccagacaacg tagttcaaca gtatccgtcc ggccaacaca
     8161 ccttcccgac accgccgccg aaggccatgg cccatcaagc ggattttcca tgcgccctca
     8221 gcgccgtacc tacagcaaat ctttcaaggc ccaggtcatt caggagtgtt ctgaacccgg
     8281 tgcctccatc gcaaacatcg ccttgggcta cagcctcaat gccaacctcg tccacaaatg
     8341 gattcgccag cttacccaga aaaccacggc tatccagcca gcgttcatcc cgttgccttc
     8401 tcaaatgctc ggcgcgggtt cgcatgctca agcgcgctgc tgcgatcatg agtttgatcc
     8461 agtcggcccg taataatggg cacgatccgt atgcctatct caaggatgtg ctgagacggt
     8521 tgccgacgca gcgagctagt gagattgcag agctgctgcc gcataggtgg catccggcat
     8581 agtcgcgcaa gacggtttgc ccgcatgctt acgttcaaca gctcaagcac cgtccgccct
     8641 tcgcgggatc gttgccgcca gagccatacg ccgaactttt ccaccttgtc gtcgccggcc
     8701 tgcggtcagt caggcaagcc caggtgcgag cctgtacata gatttgtgta attgcctgat
     8761 tttgatatgt tcaattcaac atcaaatgaa ggtaaaatta tggacgacaa acaattgcag
     8821 gctctggcta acgaactggc caaaaatctc aaaacccctg aagatctcag ccagttcgat
     8881 cgcctgctca agaaaatcag tgttgaggct gctctcaacg ctgaactgac tcaccatctg
     8941 ggacatgaaa aaaatcagcc caaattggga accaactccc gtaatggcta ttccacgaaa
     9001 accgtcacca ctaccgacgg gccgatggaa ttacgtactc cacgtgatcg tgatggttcc
     9061 ttcgaaccac aactggtgaa gaaaaatcag acccgcatta ccggcatgga caaccagatc
     9121 ctggcgctgt atgccagagg gatgactacc cgcgaaatta tctcagtctt caaagaaatg
     9181 tacgatgcag acgtctctcc ggcgctgata tcgaaagtca ccaatgccgc aatcgaccag
     9241 gttgtagaat ggcaaaaccg tcctctggat gcgatttatc ccattgttta tcttgactgt
     9301 attgtcctga aagtacggca ggacagctgg gttatcaaca gagcggtgtt cctggctctg
     9361 ggtattaacc ttgatggtca gaaagagctg ccaggtatgt ggctggccga aaatgaaggt
     9421 gcaaagttca ggctgaatat actgacggaa ctgaaaaatc gcgggatgaa cgatatcctt
     9481 atcgcctgtg tggacggcct gaaaggcttc ccggaagcga ttaatgccgt ctacccggag
     9541 acgcagatcc agttgtgcat agtacacatg gcacgcaaca gcctgcggtt cttggcgtgg
     9601 aaagactata aagcggtaac ccgtgacctg aaacaggttt accgcgcagc tgatcttacc
     9661 cagcaatagt ggacacgcgg ctaagtgagt aaactctcag tcagaggtga ctcacatgac
     9721 aaaaacagta tcaaccagta aaaaaccccg taaacagcat tcgcctgaat ttcgcagtga
     9781 agccctgaag cttgctgaac gcatcggtgt tactgccgca gcccgtgaac tcagcctgta
     9841 tgaatcaca
//
LOCUS       DAPHBJ010000166         9754 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.168, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000166 DAPHBJ010000000
VERSION     DAPHBJ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.168"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..144)
                     /locus_tag="RHZ76_003566"
     CDS             complement(<1..144)
                     /locus_tag="RHZ76_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814142.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFS"
     gene            222..1316
                     /gene="ddlA"
                     /locus_tag="RHZ76_003567"
     CDS             222..1316
                     /gene="ddlA"
                     /locus_tag="RHZ76_003567"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDV2814143.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1340..1552)
                     /locus_tag="RHZ76_003568"
     CDS             complement(1340..1552)
                     /locus_tag="RHZ76_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HDV2814144.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1812..2120
                     /locus_tag="RHZ76_003569"
     CDS             1812..2120
                     /locus_tag="RHZ76_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HDV2814145.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2179..3273)
                     /gene="yaiW"
                     /locus_tag="RHZ76_003570"
     CDS             complement(2179..3273)
                     /gene="yaiW"
                     /locus_tag="RHZ76_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HDV2814146.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3286..4506)
                     /gene="sbmA"
                     /locus_tag="RHZ76_003571"
     CDS             complement(3286..4506)
                     /gene="sbmA"
                     /locus_tag="RHZ76_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HDV2814147.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSTHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4858..6015
                     /gene="ampH"
                     /locus_tag="RHZ76_003572"
     CDS             4858..6015
                     /gene="ampH"
                     /locus_tag="RHZ76_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HDV2814148.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYPRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6016..6639)
                     /gene="iprA"
                     /locus_tag="RHZ76_003573"
     CDS             complement(6016..6639)
                     /gene="iprA"
                     /locus_tag="RHZ76_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HDV2814149.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNSEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6727..9633)
                     /locus_tag="RHZ76_003574"
     CDS             complement(6727..9633)
                     /locus_tag="RHZ76_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV2814150.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQTNAANYDTWTYIDNPVTALD
                     WDHMDKVGTVDGNYVNYSGFVYYNNTNGDFDQSFNGDTVNGTISTYYLNHDYADSTAN
                     QLDISNSVIHGSITSMLPGGYYDRFDADGNNLGGYDFYTDAVVDTHWRDGDVFTLNIA
                     NTTIDDDYEALYFTDSYKDGDVTKHTNETFDTSEGVAVNLDVESNINISNNSRVAGIA
                     LSQGNTYNETYTTESHTWDNNISVKDSTVTSGSNYILDSNTYGKTGHFGNSDEPSDYA
                     GPGDVAMSFTASGSDYAMKNNVFLSNSTLMGDVAFTSTWNSNFDPNGHDSNGDGVKDT
                     NGGWTDDSLNVDELNLTLDNGSKWVGQAIYNVAETSAMYDVATNSLTPDATYENNDWK
                     RVVDDKVFQSGVFNVALNNGSEWDTTGRSIVDTLTVNNGSQVNVSESKLTSDTIDLTN
                     GSSLNIGEDGYVDTDHLTINSYSTVALTESTGWGADYNLYANTITVTNGGVLDVNVDQ
                     FDTEAFRTDKLELTSGNIADHNGNVVSGVFDIHSSDYVLNADLVNDRTWDTSKSNYGY
                     GIVAMNSDGHLTINGNGDVDNGTELDNSSVDNVVAATGNYKVRIDNATGAGAIADYKD
                     KEIIYVNDVNTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSAN
                     TNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVD
                     TKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYS
                     HFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSISGLFQSGDD
                     YQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNG
                     DSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2544 a         2417 c         2278 g         2515 t
ORIGIN      
        1 agaaaaaaac tcaccgcatt gccatttaag ccaggaataa attagcttcc cgttcatcat
       61 ttttgtgatc ttgcgcacat ctttgagata acgcactttc ttcaccagta cgttgcacgc
      121 tttgtcagca gaagattgca acaatctttc agaaggccgc gaatcccccg ttttgtcgtg
      181 gtaatgtatg cactttgtgt tagacgggat aggtttttaa gatggaaaaa ctgcgggtag
      241 gaatcgtttt tggtggtaaa tcagcggaac atgaagtgtc tctgcaatcg gcaaaaaaca
      301 ttgtcgatgc cattgataaa agtcgcttcg acgttgtgct gctgggcatt gataaacaag
      361 ggcaatggca cgtcagcgat gccagcaatt atctgctaaa tgcagacgat cctgcccata
      421 ttgcgttgcg cccttcggcg accagccttg cgcaggtgcc aggtaaacat gagcatcagc
      481 ttatcgacgc gcaaaacggt cagccgttgc cgacggtgga tgtcattttc ccgattgtcc
      541 acggtacgct gggcgaagat ggttccttgc agggaatgct gcgggtcgcc aatttaccgt
      601 ttgtaggttc tgatgttctg gcttcagcag cctgtatgga taaagatgtc accaaacgtc
      661 tgctacgcga tgccgggctg aacattgcgc catttattac cctgacgcgc gctaatcgtc
      721 acaacatcag ttttgccgaa gtggagtcta aactggggtt accactgttt gtaaaaccgg
      781 ctaatcaggg ctcttctgtt ggtgtcagca aagtaaccag tgaagaacag tacgcaattg
      841 ccgtcgatct ggcgttcgag ttcgatcata aagtgatcgt tgagcaaggg atcaaaggtc
      901 gtgagatcga atgcgcagtt ctgggcaacg acaatccgca agccagcacc tgtggcgaga
      961 tcgtactcac cagcgatttc tatgcctacg acaccaagta cattgacgaa gatggcgcga
     1021 aagtggtagt tccggcagcc attgcaccag agatcaacga taagatccgg gcgattgccg
     1081 ttcaggctta tcagacgttg ggatgcgcag gcatggcgcg tgtagacgtg tttttaaccc
     1141 cagagaacga agtggtgatc aacgagatca acacactgcc tggcttcacc aacatcagta
     1201 tgtatccgaa gctgtggcaa gccagcggtc tgggttacac cgatctgatc acacgtttga
     1261 ttgagctggc gctggagcgt cacgctgcgg ataacgcatt gaaaaccaca atgtaatatt
     1321 ttaacgcccg ataatattct tattgggcgt tttcagtttc ttcatcgcga cgacgaataa
     1381 tcaaccccgc cagccagaaa ctaatcaccc acgtcaccag accgacggca taggtctgcc
     1441 aaccttttgc ttcaaatccc agtaacccca ccacgccatt aagaatgaat ataaggccaa
     1501 tggcgaacgc atagtagtgc cagtcacggc ggatttttac aggcagattc atggcagctc
     1561 cgttaacgaa aaacaacatc cttgcatatt ctgtaacaac cgtctgtggt gtagacaaca
     1621 ttttcgtaat tgttaataaa tttaacttaa ggttgccatt ttgtgactca caacgaagat
     1681 tcacgcttcg ggaatattcc caacatcaat ttaccatgat tctgatattg acaatctaaa
     1741 tgacctgtca gacttgccat aagccgctgg tatcacacca gaagagaagc agatgacttc
     1801 cggaggtttt tatggctgat ttcaccctgt caaaatcgct gtttagcgga aaatatcgca
     1861 atgcctcttc aacgcctggc aacattgcct atgcgttgtt tgtgctgttt tgcttttggg
     1921 ctggggcgca attgctgaac ctgttagtgc atgcgcccgg cgtctatgag cgtttaatgc
     1981 aggtccagga aacaggtcgc ccacgggtgg aaattggttt aggtgtcggc accattttcg
     2041 ggctgatccc gtttttagta ggctgcctca tttttgcagt ggtggcgcta tggctgcact
     2101 ggcgacatcg ccgccagtaa gatttacgtt gccagatacg gcctaaaacg ccttatctgg
     2161 catgacattc ctgtttgatc actgtttcat acaacgcgcc cgccgttcgt ctacgcgctt
     2221 cgcaaaccag gccgtagtca ggttgcgggt gattttcggg ctttccagtt gaatgccagg
     2281 caacatttcg cggggtagtg atttacccgt tttcgcttcg gcaagttgat aaactttctt
     2341 gtacagcgcc gtctcctcaa aagagaagct gtcacctttc tctaactgac ggcgaatttc
     2401 gctgtcgctc atccccagtt ttcccgccag tttgcgcgtt gccagttcgg ttttcccggg
     2461 ttctttactg tcgtaacgaa ttaaatcacc gtccagtgcc agcttcacgc cgctggcctt
     2521 actgactgcg ttctgaaacg cggcattacg gctggcgtac cagccagcgt taaaatcagc
     2581 aaagcggtat atcggtgcgc tatagctggc gggataattc agtaaatggt aagtaccaaa
     2641 ccacaacccg ccgcggcgac tgaagacttc ctgacgaact gtaccgtcca ttttccacgg
     2701 ataccctttg gtatgctgtt cggcaaaagc aatgctgacc tgcatcggcc caccggtgcg
     2761 caccggatta agcgaaccaa acagcgtctg ccccattggt accatgctga tcagatcgtc
     2821 aaaaatcgcg ctcaattgct tttctgtacg caccgaatct aaccgttcgc tataactttt
     2881 gccgtttggc gatttgattt ttagcgcggt atgaaccaga aaagcaggaa tgtgcatccg
     2941 ttcagcacga cggtcaatct cttgccaggc gatcttgctt aagcccggaa cggccggatc
     3001 tgcctgataa tttgactcct gctgcgccac cgccagcacc gagcagacgt tttccagcgt
     3061 cggtgccagc ccctgacttt caaaggtggt ggcgagatct ttcgcccagg catcgcggtc
     3121 tttcacgctc gcaggcatct tctgccgcac gacgctcgcc acatctatcg ctttttcgcc
     3181 ctttttcaac ggttgcggag cctgactact acaccccgct aaaaccagaa ccgccaacaa
     3241 tgacagcgaa gatagaggat tgacgcgcga cattacttct cctttttagc tcaaggtatg
     3301 ggttacttcc tgaattttgt caccatccag ttcatgttca aagctgcgca gacgtttgta
     3361 gatagacatc aactcaacca gtgtggtcca tgagttaatc aggtactgga aagcaccgcg
     3421 aacctgacca aaaacgttgg taatctgcgt catcaggccg agcgtaatcg taccggcaac
     3481 aattgacgga aacagcaaga acaaaccgaa aacgttatcg acctgcaaat agagaatgcg
     3541 ggcgatgttg aaatacatat agtgaaaata gaggcggaaa tagtttttcc gcacggcgct
     3601 aaacagctcg cgtaccgtag gcggcgtcgc acgcgtggca tcgtcttcac cataaaccag
     3661 ctctttacgg taggcggcct ctactcgctg gtttttaaac tccagccccg gcagtttgat
     3721 ccctaccact gccagcaatc cggtccccat cagcgaccag acgatagcgg caatcaccag
     3781 accatacgga atgtgcccga taatcggcag ctccggcaca tgcgtggaga gcgttaccag
     3841 caccggcagg aaggcgatca acgtcatgat ggcgttgata aaactgacgc ccatattctc
     3901 cagcgttgaa gcaaaacgca tggtgtcttc ctgcacacgc tgtgcggccc cttcgatatg
     3961 acgcagttgt tgccagttcg ccatgtaata ttcgttcatc gccgtacgcc agcggaacac
     4021 gtagtgactg acaaagaagt tgttcagcac actgatcacc acagcgatca gcgcaatccc
     4081 cagaaagacg cccacttcgc ggtaaaattg ttcgatagtg actttatgcg gcgaacttag
     4141 cgcggtttga atcagatcat agaacggtgc ataccaggcg ttgacggcga ccccgacttc
     4201 caccaaaaac caggtgacga agatgatcag tgcagtaccg agtattgacc agtattgcca
     4261 acggtgcggg ctgtagataa accagaaaaa tgcaaaaagt cctacgcaaa caatgtagta
     4321 agcgtaaaaa atcaggaaat ccaacgacca gaaacgcgcg gcgctaatcg ggatctgccc
     4381 ggaagcgcca gtgatacgcg ccacccagtc tcccccaccg gcttgccaga agataacggc
     4441 aatcaatgcc caaacaaagg ccgagagaaa aaacgctccc ggctttggga aaaaagactt
     4501 aaacatatgc actcctgcta acttcttatc gttttaacaa ctgctgtgta ttaaccgcat
     4561 gaccactaca aagcgtcagc caaatgaaac cgcccattgt gacccatagc aaggcgctta
     4621 gttcgccagc ctgaccataa gaattgtttc gacggatttc acaaaccaaa gattgtttac
     4681 ataagcggat taacacgcgc aggatcacaa atgcaggcat tggtgagcag gaaaaagatc
     4741 gtgtttacgc gccgttacgt ttttaacact attattgcac taattcgccc tttgcaatcc
     4801 atcaatgagt agtataaata cgctcagtta ccttcattca atttatggac accaccgttg
     4861 aaacgtagtc tgcttttttc tgccgtgctg tgtgcggcgt cattgacctc cgtccatgcg
     4921 gcgcagccga tcactgaacc ggagtttgcc tctgatattg tcgatcgtta tgccgatcat
     4981 attttttacg gcagcggtgc cacggggatg gcgctggttg ttatcgacgg taaccagcgc
     5041 gtctttcgta gttatggcga aacgcgacct ggtaataacg ttcgcccgca gctggattcg
     5101 gtcgtgcgta ttgcttccct caccaaacta atgaccagtg aaatgctggt gaaattgctc
     5161 gaccagggga ccgtaaagtt aaacgatccg ctaagtaaat atgctccgcc aggcgcacgc
     5221 gtgccgacct acaatgggac gcccattacc ctagtgaatc tggcaaccca taccagcgcc
     5281 ctgccccgtg aacagcccgg tggcgcggca catcgtccgg tgtttgtctg gccaacgcgc
     5341 gagcaacgct ggaaatacct ttctacggcg aagctgaaag ccgcgccagg cagtcaggcg
     5401 gcgtactcta accttgcgtt cgacctgctg gcagatgcac tggcgaatgc gtccggcaag
     5461 ccttataccc agttgtttga agagcagatt acccgtccat tggggatgaa agacaccacc
     5521 tacaccccct caccggatca gtgccgccgt ttaatggttg ctgagcgcgg tgccagtccg
     5581 tgcaataaca cgctggcggc aattggcagc ggcggcgtct attccacgcc tggcgatatg
     5641 atgcgctgga tgcagcagta tttgtcatct gatttttatc agcgcagtaa ccaggcagat
     5701 cgcatgcaga cgttgattta tccgcgcgcg cagtttacga aagtgattgg catggatgtc
     5761 cccggcaaag cggatgcgct tggtttaggc tgggtatata tggccccgaa agagggtcgt
     5821 ccggggatta ttcagaagac aggcggtggt ggcggtttca ttacctatat ggcgatgatc
     5881 ccgcagaaga atattggcgc gtttgtggtg gtcactcgtt cgccgttgac gcgctttaaa
     5941 aatatgagcg atggcatcaa tgatctggtg accgagctaa gcgggaataa accgttggtt
     6001 atccccgcat cctgattaat actctgaagg caaacggttg atagcgacca gtttgccttt
     6061 attcatttcg atatagccgc ctttacgcaa agcagcgaga acttctgcga cgacagaacg
     6121 cgatatgcgt gtacgttgat ggatataatt catcacacca atacgtgatc gcaattcttc
     6181 attccagtca atcattgata ataatgttgc gcggatttgt tcgtaggaat tatgcccaat
     6241 gagctgcacg tcgcgtaatt ccagaattct attttgccag gctaaccagt aaaaagcgtc
     6301 tcgccagagt tgattttgtt caataagcgt aatggtttgt ttggctggta gatgatatcc
     6361 cgtacaattt ccttctgata ttaatttgta tggtatatcg tttttcatta aaccatcagc
     6421 cagccccata atataaggag cctgggtaat accgataagt acgttttctt ctctacgcag
     6481 agagataact ccctctaaaa taacgaaagt atcttcgcta ttgacatcac tggaaaatat
     6541 aacttgcttt tcactattaa actcgaagcg cgtaccgtat ctggacaaac atttatcgag
     6601 cttaccaaat tcctgaagag gtttaactac agataacatt tgcgcgtcct ttgcagtaat
     6661 gcccgtcaaa tccttgacgg gcattattta gattaaatta ccagaatttc ttcggagtga
     6721 agaatattac caggtatatt taacacccac gttcgcggac caatcttgat ctacgtcacc
     6781 accaccgagg tagttagcat cggtataggc gctgaagttc ttggtgaagc tgaactgagt
     6841 acccagacca acacgtaccg cagacccttc agtaccgtta tcgatggaat caccgttcac
     6901 atcgttatcg ttgttagagt cgtcgtagac gtaagccagt ttgaagtacg gagtcagagc
     6961 ctggtcttcg ctgtaggtga aggtataacc tgcatctaca cccagttcat aacgcatgct
     7021 gtcgtaagac tgaccgtcaa ctttcatgtc gttgctcagc tggtagtcat caccagactg
     7081 gaacagacca gaaatgctgc cgtaaggagt cacataacca gcatcaccca gtttgaagtc
     7141 gtaaccggct ttcaagccga agccccaagc gtcggagttg gtgctaccgt caacgtaagt
     7201 accgttgctc atggttgcag acaggtcgtt gttgaagtga gagtagctca agctaccatc
     7261 aacaaagacg ttgttcgcga agtgagcaga agagtagatg taggcagtct ggctatcttg
     7321 atctacctga ccagaacgat cattcatgtc acctttagcg aagcctgcag ccgcaccgac
     7381 gatccactta gcgttgttac cgtcaatttt ggtatcaaca ccgaccatga tgccgttaac
     7441 atcctgatca tagttgatgg tgccgttgtc gccgttgaag ttaccaccga agtagcttac
     7501 ccatgcgccg ccgttatcag ccagaccatg acgagagttg gtcagacgag taccaacggt
     7561 gtcttgttcc aggttccaga tattggtgtt cgcagacggg atgctcagtg ccatgttagc
     7621 gtagtcggtc agctccatct gttgcagaac aacggtgtta ccgcgctgtt cagcctgata
     7681 ggtgtatgca cccaggtcag ctttgttagc agcagagaag gtcgcgttgg tgttgacgtc
     7741 gtttacgtag ataatttctt tatctttgta atcagcgata gcgccagcgc cagttgcgtt
     7801 gtcgatacga actttgtagt taccggttgc agcaacaacg ttatcaacag agctgttatc
     7861 cagttcagta ccgttgtcta cgtcgccgtt accattgata gtcaggtgac cgtcagagtt
     7921 catagcaaca ataccgtaac cgtagttaga cttggaagta tcccacgtac ggtcgttcac
     7981 cagatcagcg ttcagaacgt aatcgctgct atggatatcg aacacaccag atactacgtt
     8041 accgttatgg tcagcgatgt tgccgctggt cagttccagt ttgtcagtac ggaaagcttc
     8101 agtatcgaac tgatcaacgt tcacatccaa tacaccaccg ttagttacgg tgatagtatt
     8161 ggcgtacagg ttgtaatcag ccccccaccc agtagattcg gtcaacgcaa cagtactgta
     8221 ggagttaata gtcagatgat cggtatcaac gtagccatct tcaccaatgt tcagcgaaga
     8281 accgttagtt aaatcgatag tatctgaagt taatttagat tccgaaacat taacctgaga
     8341 accattatta actgtcaagg tatcaacgat ggaacgacct gtagtatccc attcagaacc
     8401 gttattcaac gctacgttaa atacaccgct ctggaagacc ttgtcatcaa caacacgttt
     8461 ccagtcattg ttttcatatg ttgcatcagg agtaaggctg tttgtagcaa catcatacat
     8521 tgctgacgtt tcagcaacgt tataaattgc ctgaccaacc cacttgcttc cgttatcgag
     8581 agtgagattt agttcatcaa cgttgaggct atcatcagtc caacccccgt tggtatcttt
     8641 caccccgtca ccgttggaat catgaccatt cggatcaaaa ttactattcc aggtgctggt
     8701 aaaggcaaca tcacccatca gcgttgaatt gctgaggaat acattgttct tcatcgcata
     8761 gtcggaacct gaagcagtaa aggacattgc aacatcaccc gggccagcat aatcactcgg
     8821 ttcatcagaa ttgccaaagt gaccagtttt gccataagta ttgctatcca ggatataatt
     8881 tgaacccgaa gtcactgtgg aatcttttac agagatattg ttatcccaag tatgagattc
     8941 ggtagtgtag gtttcgttgt aagtattacc ttgagataat gcaatacctg caacgcggga
     9001 gttattggaa atattgatgt tactttctac atcaagatta acagcaacgc cttcacttgt
     9061 atcaaatgtc tcatttgtgt gcttggttac atcaccatct ttataagaat cagtgaagta
     9121 aagagcttca taatcatcat caatagtagt gttagcaatg ttcaaagtga aaacatcacc
     9181 atcacgccag tgtgtatcaa caaccgcatc agtgtaaaaa tcatatccac ccagattatt
     9241 accatctgca tcaaaacgat cataataacc gccaggcagc atagaagtaa tcgaaccgtg
     9301 aatcactgaa ttactgatat caagctgatt agcagtactg tctgcataat catggttcaa
     9361 ataataggtt gagattgtcc cgttaacggt atcgccgtta aaggactgat cgaaatcacc
     9421 attggtgttg ttgtaataga caaaaccact atagtttaca tagttgccat ctacagtgcc
     9481 taccttatcc atatgatccc aatcaagtgc tgtaacggga ttatcgatat aagtccaggt
     9541 atcatagttt gctgcattag tctgagaggt gatagccaga gtgcaagcca atgctaattg
     9601 tgatactaca agtttctttt tccaggagtg catttgtcat ccctcctcag ggacgtaata
     9661 agttgatcca tcaattatca atgcatagaa aacatattat gtttcgcgct aatgattatg
     9721 ctgcgttaca gacgaatggt tcaattattc tttc
//
LOCUS       DAPHBJ010000167         9754 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.169, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000167 DAPHBJ010000000
VERSION     DAPHBJ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.169"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(472..579)
                     /locus_tag="RHZ76_003575"
     CDS             complement(472..579)
                     /locus_tag="RHZ76_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic polypeptide LdrB"
                     /protein_id="HDV2814151.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(984..2084)
                     /gene="chaA"
                     /locus_tag="RHZ76_003576"
     CDS             complement(984..2084)
                     /gene="chaA"
                     /locus_tag="RHZ76_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HDV2814152.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPTANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            2354..2584
                     /gene="chaB"
                     /locus_tag="RHZ76_003577"
     CDS             2354..2584
                     /gene="chaB"
                     /locus_tag="RHZ76_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HDV2814153.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            2742..3437
                     /gene="chaC"
                     /locus_tag="RHZ76_003578"
     CDS             2742..3437
                     /gene="chaC"
                     /locus_tag="RHZ76_003578"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HDV2814154.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(3481..3834)
                     /gene="ychN"
                     /locus_tag="RHZ76_003579"
     CDS             complement(3481..3834)
                     /gene="ychN"
                     /locus_tag="RHZ76_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HDV2814155.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            4020..5414
                     /gene="ychO"
                     /locus_tag="RHZ76_003580"
     CDS             4020..5414
                     /gene="ychO"
                     /locus_tag="RHZ76_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HDV2814156.1"
                     /translation="MSRFVPRIISFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(5415..6065)
                     /gene="narL"
                     /locus_tag="RHZ76_003581"
     CDS             complement(5415..6065)
                     /gene="narL"
                     /locus_tag="RHZ76_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HDV2814157.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(6058..7854)
                     /gene="narX"
                     /locus_tag="RHZ76_003582"
     CDS             complement(6058..7854)
                     /gene="narX"
                     /locus_tag="RHZ76_003582"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HDV2814158.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVQVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            8193..9584
                     /gene="narK"
                     /locus_tag="RHZ76_003583"
     CDS             8193..9584
                     /gene="narK"
                     /locus_tag="RHZ76_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HDV2814159.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         2351 a         2395 c         2503 g         2505 t
ORIGIN      
        1 gccaggtcgt cccagaaaat catggcaaac tgcgcgagcg tcatatagcc gccttgttgt
       61 aatgacaaca ttttgcggct attcttgaat tgttctggtt caagattagc ccccgttctg
      121 ttgtcaggtt gtacctctca acgtgcgggg gttttctctt tccagcaacc aatgccacca
      181 gggataaagc ccccgcaaca ttgcgcctca ccggataacg ccggcttggt gtggatacta
      241 cgactcaatt catcttcact tcatccctga aatgtttgca atgaagagtg cattccggtt
      301 tttcaacagc tgttacagtc atttcatgag tgctctggat gaggcttcca gctcgggttg
      361 ccaatattta cttgtggaag tgataaagac aaaaatggcc gcaggctgtt acccctgcgg
      421 ccggtttcgg gcgcatattg ccatcacggc agcctgacgc ccgttttcac cttacttccg
      481 gttacgccac cagccgacaa tcgctgcggt aataattccc gccaggatcg gtgccgccag
      541 gtcgtgccag aaagtcatgg caaactgcgc gagcgtcata tagccgcctt gttgtaatga
      601 caacattttg cggctattct tgaagtgtct ggtttcaaga ttagcccccg ttttgttgtc
      661 aggttttacc tctcaacgtg cgggggtttt ctctttccag caaccaatgc caccagggat
      721 aaagcccccg caacattgcg cctcaccgga taattccggc ttggtgtgga tactacttct
      781 caattcatct tcacttcatc cctgaaatgt ttgcaataaa gagtacattc cggcttttca
      841 acagctgttg cagtggtttc gtgcaggttg tggtacaggc tcgcaattct gattaacgag
      901 ctgtccaggt agtatgaaga aatacaataa gcaggaattt atcttatctc ccccgatttc
      961 cgtcatcgga ggagatataa ctgtcaggca aatatcgtca tcaaataggc ggcaaacagt
     1021 gccagatgcg ctgcgccatt gagcacgtta gtacgtccgg tggagaagga gatatggcac
     1081 agcactaaag aggccaccat caccaccatt tctggcgcac caagtgcaaa ctgtaattcg
     1141 ttacccgtca taaaggcaat tagcgtgacg acaggtacgg taagcgaaat ggttgctaac
     1201 actgaaccaa agaacagatt catcgcgcgc tggacctggt tgttcaacac tgcttttaac
     1261 gcacctaaac cttccggcga cagaatcaac agagccacca ggaagccagt aaaggcgaca
     1321 ggggcattca tgctgtcgag caatgtctcc agcgggctgg cattcatttt ggtgactgca
     1381 ataacggcaa tcagatggat aatcaaccag atagcatgcc acacgctgct atgggcagac
     1441 ggtttaccgt gatgcgggtc gtcgtcatca ctgtcatctt cgtgctcgta gacaaacaaa
     1501 ctttgatgcg ttttggtctg gatcagcaaa aatacgccat acattgccgc agaaattaat
     1561 gctaccagta acgcctgacc ggttgaaaaa ttcgccgtag gcagagccat tggaaatacc
     1621 agtacgatta tcgccagggg gaacagcgca attaaatact gcttgatacc aaacagattc
     1681 atatattggg tggcaaactt acgaccgccc aacaataatg aaaagccaac cagcccaccg
     1741 gtaacaatca taatgattga atagagcgta tcacgcatta gcgttggcgc ggcgtcgccg
     1801 gttgccatta aagctgaaat caaactgact tcaagaataa ccactgaaag gctaagaata
     1861 agcgaaccgt aaggttctcc caggcgatgg gctaatacgt ccgcatgacg gacaacacta
     1921 aaggcgctac ttaaaatacc aataagcgca agaagattga tggcaatgac cactggtagt
     1981 gtctggctgc ttccccacag gaacagcact accagcgcca gaaccgggaa aataagcgaa
     2041 gtctccttgt ggcgggtttt taccgcctct tgagcatttg acattatggt tatccctttg
     2101 cagatgaatt tatcgaaaat gtaaaaaata ggtaggaaaa ataacagaaa gtgtctggat
     2161 atcggtaaca ttttacgaat ttttacccca ctgtcgattt tttactcatt gggacataaa
     2221 aataagtatt acgtttagac aatgtttgtt ttagcgtttc ttaagaacag tctgacatga
     2281 aaattcttat gttttggcaa gagtagatat tgttgaccac acttaatgtt caactttgta
     2341 aaaggagtca acgatgccgt ataaaacgaa aagcgatctg ccggaaagcg taaagcacgt
     2401 tctaccgtct catgcccagg atatctataa agaagcgttc aacagcgcgt gggatcaata
     2461 taaagataaa gaggatcggc gtgatgacgc cagtcgcgaa gaaaccgcgc ataaagtggc
     2521 ctgggctgct gtgaagcatg aatatgccaa aggggatgat gataaatggc ataaaaaatc
     2581 gtaaaaccgc tagcttagtt aaagctattc gtgcggtgtt gccttgcaag tggtccgtgg
     2641 attgcatatt gtcccgttag tggtttcaaa atgagcagta aaaatgtccg gaagacacca
     2701 aaaagttgtc gcagggaagt atgcagtggc ggaggtgtaa ggtgataacg cgtgatttct
     2761 tgatgaatgc cgattgtaaa acggcatttg gtgccattga agaatcactc ttatggtcag
     2821 cagagcaacg ggcggcttcg ctggcggcga cgctggcttg tcgacctgat gagggaccgg
     2881 tgtggatctt cggttatgga tcgttgatgt ggaatccggc actggagttt accgagtcgt
     2941 gcaccggtac actggttgga tggcatcgcg cgttttgcct gcgcctgacc gccgggcgcg
     3001 ggactgcgca ccagccggga cggatgcttg cactgaaaga gggcggacgc actacaggcg
     3061 tcgcctatcg actgccagaa gagacgctgg agcaggaact aaccctgttg tggaagcgcg
     3121 agatgattac cggctgttat ctgccaacct ggtgtcagct tgatcttgat gatggatgca
     3181 cagtaaacgc cattgtgttt attatggacc cgcgacatcc agaatatgaa tctgacactc
     3241 gcgctcaggt catcgcgccg ttgattgcgg cggcgagcgg tccgctggga accaacgcac
     3301 aatacctgtt ttcactggaa caggagctta tcaaactggg aatgcaggac gatgggctga
     3361 atgatttgct ggtatcggta aaaaaactgc tggcggagaa ttatccggat ggtgtgttac
     3421 ggccgggatt cgcctgagta aacttccctc atagtggggc gtcagacgcc ccctcaaaca
     3481 ttaaaatgtg agcactttat cggctgacag cgtccattgc gccagttcca caagagtacc
     3541 gatttccacc ccatcaataa gaggaagtgt gctaatcccg cgcccgtcgg tacaggtttt
     3601 gcacaatttc accggtacat tctgagcggt aaggatctcc agcatttgct gaatgttgta
     3661 gccttcccct ggtttttgcc cgcgcaaccc ggctgtgacc gcatcagaca tgaggaacag
     3721 acgcagatcc agattgctct cctgctctcg taacgcaatg gccagccgca agctgttaaa
     3781 caaggattcg ctcccgtaag gtgcgccatt ggcaacgatc acgatttttt gcattattta
     3841 ctcctgtatt cagggaatta gacactcatc ttctatctta ctgcttctgc agcgtctgac
     3901 caatcggtca catttttaag gattttcctg aaagcgcgag aaaatacgac aaaagttgcc
     3961 agtaatcgtt attcttaaag gctatggttt ttcatttttt accggaagtt accgacgttt
     4021 tgagccgttt cgttcctcgc attatttcgt tttatttact cttgctggcg gcaggcggta
     4081 cagctaacgc acaatctacc ttcgagcaaa aagcggcaaa tccctttgat aataacaatg
     4141 atggtctgcc ggatttaggc atggcacctg aaaatcatga tggggaaaaa cactttgctg
     4201 aaattgtgaa agatttcggc gaaaccagta tgaatgataa cgggctggat actggcgagc
     4261 aggcaaaagc tttcgcattg ggaaaagtcc gcgacgcgct tagtcaacag gttaatcagc
     4321 acgtagagtc ctggctatca ccgtggggaa atgccagtgt tgatgtcaaa gtggataacg
     4381 aaggtcattt taccggcagt cgtggaagct ggtttgtgcc gttacaagat aatgatcgtt
     4441 atctcacctg gagccagctt ggtcttactc agcaggatga tgggttggtg agcaatgtgg
     4501 gcgttgggca acgctgggcg cgcggcaact ggctggtggg ttataacact ttttatgaca
     4561 acttgctgga cgaaaatctt cagcgagcgg gctttggtgc cgaagcgtgg ggcgaatatt
     4621 tgcgactatc ggcaaacttt tatcagccat ttgctgcatg gcatgaacag acagccacgc
     4681 aggaacaacg gatggcgcgc gggtacgacc tgacagcccg gatgcgcatg ccgttctatc
     4741 aacacctcaa taccagtgtc agcgtagaac agtattttgg tgatcgtgtt gatttgttta
     4801 actctggtac gggttatcac aatcccgtcg cgttgagtct gggattaaat tacacccctg
     4861 tgccattagt cactgtgacg gcccagcata aacagggtga aagtggcgag aatcaaaata
     4921 acctcgggct gaatcttaat taccgctttg gtgtaccgct caaaaaacaa ctttctgcgg
     4981 gcgaggttgc cgaaagtcag tcgttacgtg gtagtcgcta tgataatccg cagcgaaata
     5041 atctaccgac tcttgagtac cgacagcgaa aaacgttaac ggtgtttctg gcgacaccgc
     5101 cgtgggatct aaaacctggc gaaacagtgc cgctgaaatt acaaatccgc agtcgttacg
     5161 gtattcggca actgatttgg cagggcgata cgcagatatt aagtttgacg ccaggcgcac
     5221 aagccaacag cgcggagggc tggacgctga tcatgcctga ctggcagaac ggggaagggg
     5281 cgagcaatca ctggcgattg tcggtggtgg tggaagataa ccaggggcag cgtgtctcct
     5341 ccaatgagat cacgctaacg cttgtcgaac cgttcgacgc attgtcaaac gacgaactgc
     5401 gctgggaacc gtaatcagaa aatgcgctcc tgatgcaccc ataccgctgc ttccacgcga
     5461 gacttgagct tcattttctt cagcatgtgc ttgacgtgca cttttactgt gctttcggtg
     5521 atatccaggc ggcgggcaat catcttgttc ggcaaaccct gggcaatcag cttgagaata
     5581 tcgcgctcgc gtggggttaa ctggttaaca tcgcgctcag tagtggcacg gttagcgcgc
     5641 aagctggcgg ccagaacagg cgttaatgct tcgcttaata ccatttcgcc agcagctgcc
     5701 tgatgcaatg ctttcagcag atcttccggt tccatatctt ttaacagata gccatccgcg
     5761 ccgcgtttca gtgcggtgac cacatcttct tcatggttag agacgctgaa taccacaatg
     5821 cgccctgaga gggacttttc gcgcagttta tccagcgttt ccagaccgtt catgccgggc
     5881 atattgagat ctaacaggat cagatcggga tcaagagact ccgccagttc aataccctgt
     5941 tcgccattac tcgcttcgcc aaccacggtg atatctggtg ccatactgat aagctgtttt
     6001 acgccagttc gcagcatcgg gtgatcgtca atcagcagga tagtagccgg ttcctgatta
     6061 ctcatgggta tctccttgga cgtctgtgaa agttttttcg ggaataaagg tgaccaccac
     6121 ttcggtgcca cctgattcac gacggcggac gcggcaatcg cctcgtaaac tttgcgcacg
     6181 gtcgcgcatt attatcatgc cgtagtgatt gctgcggatg gcattttcag gcacgccgca
     6241 gccgttatcc tggacggtca gtttgacctg attatcgttt tgcgccaccg tcaccacgac
     6301 ctcactcgct tgcgaatgtt tgagggcgtt acttaatgcc tcacgggcaa tttgcaacaa
     6361 gtggattgcc tgatgtgaag gcaccagacg aggcggcaat tgataatcca gcttcaccgg
     6421 gaagccaaat ttggcgctgt actcttcgca actcgcttcc agcgccggac gtaatccagg
     6481 ctcggtgagc tgcaagcgga atgtggtgag caattcacgc aactgcgccc aggatgcatt
     6541 cagttcgtta cggatctgac ttaacagttc gcggctgctt tctggcagcg catcgccctg
     6601 catctgtaaa caactcacct gcatcttcat gcaagagaga gattgggcaa tagaatcatg
     6661 cagttcgcgc gcaatggtgg cacgctcttc catcacgatc aactgttgct gacgttcctg
     6721 atgccgatcc agcgccagcg tggcggtgag ttgttcaacc agggtatcca ccagttgttg
     6781 ttgatcatgg ctaagatgac gcccctgcgg tagggtcgcc agcaaaatac cgtactgcgt
     6841 atgtgagtca gccagccgcc acttcagggt tgtgccgcga tcgccaacgg gtaatacgcc
     6901 gcgcgggcag agctggcagc ctttatcatc acaagtcata tctggctggc aggtaaactc
     6961 ctgatgattc tcttcatcat cagtgtcata cacccgcaat tcgatatcac gtagcagggt
     7021 taaattctgt aagccgttga gtacaggtga caggcgttca cacagcgggg cgcgggaatg
     7081 caaacggcgg ttagcctgcc ataaaaaaga gaggatctga tttttatgct ccagcccggc
     7141 ggttttctcc tgaacccgct gctcaagtac ggcataactt tcggccagtt ctgcagacat
     7201 attgttcaat gcagttccaa gcatcgccat ttcgttgcgc ccgctgatgt tcgcgcgttg
     7261 ggtaaaatcg cgatgactga cggcactcgc cattgccagc agttgccgcc acggttgtag
     7321 cagtcgcgcc cgcaaccaga taatagtgaa caccagtaaa agtgccataa ataccgccat
     7381 tacccgatgg acctgtacca ctgtctcaat gcgcatttcc gtggtgcggt caaaaccaga
     7441 taccagctga tcaagcccgg caacaaactg gctgacatcc gctgacaccg tttcgcggtt
     7501 ttgtgcacgc atcagtgcag ggatcagttc attacgccag taatcttgta aaccctgtaa
     7561 ttgcgccagt tgtccgtctc gttctgctgc tcgagtcaac tcggcgctaa atgccgtttg
     7621 ttccatctct ttaattaagg gcttgtcttt ctcgcttaat ggcactgctg ccaacagacg
     7681 gtaactttgc atgcgcagcg atcccgcttt gttgatcgca tgggcgctgc cctgaacgcc
     7741 ttgcaccagc cagccagaaa ccgccatccc tgccagtcca atagcagtag aaagcaacac
     7801 aataagcgca acctgattaa ccagggtgag cggagagaga caacgtttaa gcatgtaaac
     7861 ctcttccttc aggctttaaa tgagcaataa ccttaatgaa tgtgacgata cattctggaa
     7921 tggcagtatt ctcggctatt ggctgaagta tacccatacc cggaaagagt tactccttat
     7981 ttgccgtgtg gttagtcgct ttacatcggt aagggtagga attttacagc accgtgaaaa
     8041 atctcataat ttttatgaaa tcactgtact cactatgggt aatgataaat atcaatgata
     8101 gataaagtta tcttatcgtt tgatttacat caaattgcct ttagctccag acactaaggt
     8161 ggcagacatc gaaacgagta tcagaggtgt ctatgagtca ctcatccgcc cccgaaaggg
     8221 ctactggagc tgtcattaca gattggcgac cggaagatcc tgcgttctgg caacaacgcg
     8281 gtcaacgtat tgccagccgc aacctgtgga tttccgttcc ctgtctgctg ctggcgtttt
     8341 gcgtatggat gttgttcagc gctgttgcgg tgaacttacc gaaagtcggt tttaatttta
     8401 cgaccgatca gctatttatg ttgactgcgc tgccttcggt ttctggcgcg ttattacgtg
     8461 ttccatactc ctttatggtt cctatcttcg gtggtcgtcg ctggacggcg ttcagcaccg
     8521 gtattctgat tattccttgc gtctggctgg gttttgccgt gcaggatacc tccacgcctt
     8581 atagcgtctt catcatcatc tctctgctat gcggctttgc tggcgcgaac ttcgcatcca
     8641 gtatggcaaa catcagcttc ttctttccga aacagaagca gggtggcgcg ctgggtctga
     8701 atggtggtct gggaaacatg ggcgtcagcg tcatgcagtt ggttgctccg ctggtggtat
     8761 cactgtcgat tttcgcagta tttggtagcc agggcgtcaa acagccggat gggactgagc
     8821 tgtatctggc gaatgcgtcc tggatatggg tgccgttcct tgccatcttc accattgcgg
     8881 cgtggtttgg catgaacgat cttgctacct cgaaagcctc catcaaggag cagttgccgg
     8941 tactcaaacg gggtcatctg tggattatga gcctgctgta tctggcaacc ttcggctcct
     9001 tcatcggctt ctccgcgggc tttgcaatgc tgtcaaaaac gcagttcccg gatgttcaga
     9061 ttctgcaata cgctttcttc gggccgttta ttggtgcgct ggcgcgttct gcaggtggtg
     9121 cattatctga ccgtctgggc ggaactcgtg tcacgctggt gaactttatt ctgatggcga
     9181 ttttcagcgg cctgctgttc ctgaccttac cgactgacgg gcagggcgga agcttcatgg
     9241 cgttcttcgc ggtcttcctg gcgctgttcc tgacagctgg gctgggtagt ggttccactt
     9301 tccagatgat ttcagtgatc ttccgtaaac tgacaatgga tcgcgtgaaa gcagaagggg
     9361 gttctgacga acgtgcgatg cgtgaagcgg caaccgacac ggcggcggcg ctgggtttca
     9421 tctctgcgat tggcgcgatt ggtggcttct ttatcccgaa agcgtttggt agctcgctgg
     9481 cattaacggg ttcgccagtc ggcgcaatga aggtattttt gattttctat atcgcctgcg
     9541 tagtgattac ctgggcggta tatggtcggc attctaaaaa ataaaccgtt actcgtcata
     9601 cttcgggtta catgtgctgc ggctgcgttc attcacccca gtcacttact ttagtaagct
     9661 cctgggattc attcacttgc cgccttcctg taaaccgaat tatatagagt aaaatatttg
     9721 attatccttt gcgcggcatg atgtcgcgct tttt
//
LOCUS       DAPHBJ010000168         9615 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.170, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000168 DAPHBJ010000000
VERSION     DAPHBJ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.170"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(89..1627)
                     /gene="cadC"
                     /locus_tag="RHZ76_003584"
     CDS             complement(89..1627)
                     /gene="cadC"
                     /locus_tag="RHZ76_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HDV2814160.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQKILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(2570..2908)
                     /locus_tag="RHZ76_003585"
     CDS             complement(2570..2908)
                     /locus_tag="RHZ76_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814161.1"
                     /translation="MSVTIKDDRLRTIATFDGKTLKDDRLRTIATFDGKTLKDDRLRT
                     IATFDGKTLKDDRLRTIATFDGKTLKDDRLRTIATFDGKTLKDDRLRTIATVNGNVSI
                     VVLAFAARLF"
     gene            complement(3130..6246)
                     /locus_tag="RHZ76_003586"
     CDS             complement(3130..6246)
                     /locus_tag="RHZ76_003586"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HDV2814162.1"
                     /translation="MTQQTHTIAESNNFIVLDKFTKVMDTGDSYQSELDLERELIQDL
                     LNQGYEFISVKSPTAMLANVRDQLQRLNGVVFNESEWRRFAEQYLDSPSDSSLDKTRK
                     IHIDYICDFTFDDGRLENIYLIDKKNLLRNKVQVIQQFEQTGSHANRYDVTILVNGLP
                     LVQIELKKRGVAIREAFNQIHRYSKESFNSDNSLFKYLQLFVISNGTDTRYFASTTKR
                     DKNSFDFTMNWAKSDNTLIKDLKDFTATFFQKHTLLNVLFNYSVFDSSQTLLVMRPYQ
                     IAATERILWKINSSFKAKNWSKPESGGFIWHTTGSGKTLTSFKAARLATELDFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSDNTAGLRRNLDKDDNKIIVTTIQKLNNLMKA
                     EADLPVYQQQVVFIFDECHRSQFGEAQKNLKKKFRRFYQFGFTGTPIFPENALGAETT
                     ASVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKSLETETDEKKLSAAENQQAFL
                     HPLRIQEITQYILNNFRQKTHRTFPGAKGFNAMLAVSSVDAAKAYYTTFKMLQEEAEK
                     KSGSYKCLRVATIFSFAANEEQSAIGDITDESFDTSAMNSSAKEFLDAAIDDYNACFK
                     TNFSTDSNGFQNYYRDLAQRVKNQDIDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGL
                     MQAFSRTNRIYDATKTFGNIVTFRDLERPTIDAITLFGDKNTKNVVLEKSYEEYMQGF
                     TDAATGEAKRGFMAVVSELEQRFPDPASIDSEKEKKAFVKLFGEYLRAENILQNYDEF
                     ATLKALQKVDISDPEAVETFKAEHYVDDEKFAELQTIRLPAERKVQDYRSAYNDIRDW
                     QRREKASNDRDKSTTDWDDVVFEIDLLKSQEINLDYILGLIFDHNRQKKGKEALTEEV
                     RRLIRSSLGNRAKEGLIVDFIQQTNLDDMPDKASIIDAFFTYAQREQQREAEALIKEE
                     NLNEEAARRYIRTSLKREYATENGTELNETLPKLSPLNPQYKTKKQTVFQKIGAFIEK
                     FKGVGGHL"
     gene            complement(6367..7551)
                     /locus_tag="RHZ76_003587"
     CDS             complement(6367..7551)
                     /locus_tag="RHZ76_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDV2814163.1"
                     /translation="MSEMSYLEKLLDGVEVEWKTLEDISIKISSGGTPKTGVSEFYDG
                     DIPWLRTQEVNFCDIWDTEVKITESGVKNSSAKWIPKNCVIVAMYGATVGKIGINKIP
                     MTTNQACANIQLNEEVAHYRYVFHFLCSQYTYIKSLGTGSQTNINAQIVKNIKIPIPC
                     PDNPEKSLAIQSEIVRILDKFTALTAELTAELTAELTMRKKQYNYYRDQLLSFEQGEV
                     EWKTLGEITTKIASGRNKTRDADGKFPVYGSTGLIGFIDKAAYSGDLLLVARVGAYAG
                     LVNAVSGHFDVSDNTLIIHPSDSWNVRFAFHQLRHMNLNQYAVGAGQPLITGGLLKNL
                     KVPFPSLKEQTRIAKILDKFETITASISEGLPREIELRQKQYEYYRDLLFSFPKPDAV
                     SN"
     gene            complement(7548..9104)
                     /locus_tag="RHZ76_003588"
     CDS             complement(7548..9104)
                     /locus_tag="RHZ76_003588"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDV2814164.1"
                     /translation="MTSLQQRAELHRQIWAIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSSYIEAGDDSICYAKLDDSVITDDIKDDAIKTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVE
                     GLKLGDFHEHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTHV
                     NKIYDPAAGSGSLLLQAKKQFDDHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFD
                     IKLGNTLTEPHFRDEKPFDAIVSNPPYSVKWIGSDDPTLINDERFAPAGVLAPKSKAD
                     FAFVLHALNYLSAKGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAPNLFFG
                     TTIAVNILVLSKHKTDTKVQFIDASELFKKETNNNILTDAHIDQIMQVFASKEDVAHL
                     AKSVAFETIVANDYNLSVSSYVEAKDNREIINIAELNAELKTTVSKIDQLRKDIDAIV
                     AEIEGCEVQK"
     gene            complement(9308..>9615)
                     /locus_tag="RHZ76_003589"
     CDS             complement(9308..>9615)
                     /locus_tag="RHZ76_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814165.1"
                     /translation="LSFYENPEHWDTIPNLHLLNHSQNISKQDTSLKQWLSQPSNNYS
                     PSMLLVSDENIEFSRFPEFYNERRNALKQRLLNRVFLTTKIDSSPSTMDTDEEILTD"
BASE COUNT         2494 a         2118 c         2138 g         2865 t
ORIGIN      
        1 aaataaattg ccgcaacata ttataccaac aggaacatac aaaaactcaa caacaaatat
       61 ttccgagcat aaatcaaccc ggagttactt attctgaagc aagaaatttg tcgagataag
      121 gtacaacata aggaacagaa gtctggaata taccattttc aatccagtaa agggtgtttg
      181 cccctgggcg taaattaaag gcggtgagat atgcatcagc tgcttcccgg ttcatcccct
      241 tcatttcata aaccttgcca agcaacacat aatttagcca ggacatttca agatcaatgc
      301 cagtatttat cgcctggtaa gactcatctg ttttaccttt taccagagca ctgaccgctt
      361 ttatttgata tataatggac aggttgttca attccggcag tgtaacaatg ttatctattt
      421 ctgtgttcag tgctgctaat tgtttttcat ctaaaggatg ttgagaatgg cgcacgatat
      481 caactaatgc tttttctgct ctcgcgtagg taaattctgg ggatgattga acaatctcac
      541 ctaataattc actggcacgg ttcaatgatt tatcatcgcc atgcagtaaa taatcatgtg
      601 cctgataaaa attagttaat aacgcaccac gatgcggcaa aattttctgg agcgtctcct
      661 gcattcgttg tggccacggt tggtttaacg cttttgataa actctccagt aaatcatttt
      721 gaatcgccag ctgattaccg ttagtgatga cataacgttt atccagcatg gttgaaccat
      781 ctgcattgtc taccaatttt atcgacataa agcattgttg agcacggtat tggcgctgat
      841 taacaaacgc aatagataat gttttaccgg aactgctcgg ttcatcaatg ttgtagttga
      901 ttttgtcatg caccataaag gtggagaagg tgttaagtga tgtcgccacc aaatcaccca
      961 cgcctatcgc gtaagagagc tgatacgggg aactccagct gttacaactt ttatttacca
     1021 tattaatgtc aatatcgcgt ggattgagca aaatacgcga tttgctcata ggaagacgtg
     1081 tatcaagact tgaaaacgct accagtgcta cacagatacc taacgacaac aggaaaaaaa
     1141 accataccca aaaggtagtg aatcgtttgc ttttaactgg ggattgttca ggtggcgttg
     1201 cggtgttttg aatgttaaga ctgtgggagg gagaatctgt ggcaggaacc gcctctggta
     1261 tagggggagg cgaagatagc attatttcct ctccctcttc ttcgctgtac cagataaccg
     1321 gcaccattaa tttatagccg cgctttggta cagtagcgat atagacagga ctatcttcat
     1381 cattatcttt taatgactta cgtagttctg agatactctg cgtcacaacg tgattggtga
     1441 caatacttct cttccagaca ttatcgataa gttcatccct gctaagtact tcgccactgt
     1501 gttgagcaaa gaaaaccaga agatcgatta atctcggctc aagggtaagt tgacgcccat
     1561 tgcggctaat ttggtttatg gacggagtaa caagccattc gccaacgcga actacaggtt
     1621 gttgcataat agaaactcat tcgaaaaggg aatgatgcaa tgatcattgc cacaacctat
     1681 ttttaccatc tatagatggg tttatttaca tattattggt gaatgcaaga cgttattttt
     1741 accagccata aacttcttga ttacatagta ttacgaaagg attttactga gaaccagaag
     1801 taatattcct taccatcaaa attcatcatc tttgccaaag aaaaatgttc agaaaataat
     1861 ccatggaaaa ttgtccggag cacttactat tttaatggat tgttagtctt tgcatgagca
     1921 agcgaactga tacatttctc tttgttctca ttcagaaaat ctcatcagtc gccgttcaga
     1981 ccggtgtagt gctttatgga tgcgttacgc tcctgatgac gtcatttgac gttcaacagc
     2041 atcacggggc cgcacgacat ttcacgtcag ttagtgctat agctcaggaa caaattttcc
     2101 cgaattggga tatgcccgca aattgctggt gatgtgggga gaatctggtt gagttcggta
     2161 gaattgattt ggaggcagaa cgcttaaatc gtggcgtcct gaaacgaaaa acggacctcc
     2221 gtggaggtcc gtttatatga atttggtgcc cggactcgga atcgaaccaa ggacacgggg
     2281 attttcaatc cctttaaaag agcccaaacg atccatcagc actaacagtt ttttactggt
     2341 ttaccttgag aaaaatattt aagtttggtt attcatgact taagtattgc ttaaatacgg
     2401 cgtttcgaag ctaccagaat cctaccatta tgagcctatg cccatagaag agaaattatc
     2461 tcccgttctc gcgattcaat gactaaaaat cagacggtgc aggcttttag atactgatat
     2521 tatatctttt gggtaaagta ttaatctctg ttgagatagg ctcttagccc taaaatagcc
     2581 gagcagcaaa cgccaaaaca acaatactga cattaccatt aactgtcgcg atagttctga
     2641 gtcggtcgtc tttaagtgtt ttaccatcaa atgttgcgat ggtgcggagt cgatcgtctt
     2701 tcagggtctt tccatcaaag gtagcgatgg tgcggagtcg gtcgtctttc agggttttac
     2761 cgtcaaaagt tgcgatggtt ctgagccggt cgtctttcag ggttttaccg tcaaacgttg
     2821 cgatggtcct gagtcgatca tctttcaagg tcttgccatc aaaggtcgcg atagtcctga
     2881 gtcgatcgtc ttttatagtt acgctcataa gcattctcct gtgatacgtc cgttcagcac
     2941 tttaccatcc aaaataaagc actggtttct gtaagttctg ggcgctaatc atttgaatat
     3001 gcgtttaaaa tccatccagt tagcattaac attaaccagc gcttcatcga tcataacagg
     3061 tctgatgcag gttaaaagat acaatcttac ctataaattg cggtagcgca ttgctctgtt
     3121 atctggcatc tagaggtgcc ctccaacccc tttaaatttt tcgataaacg ccccaatttt
     3181 ctggaatacg gtctgctttt tcgttttgta ctgaggattc agcggactga gctttggtag
     3241 cgtctcattt agctcagtgc cattttcggt agcatactcc cgtttgagtg atgtgcggat
     3301 atagcgcctt gcagcctctt cattgaggtt ttcttcctta atcaacgcct ctgcttcccg
     3361 ttgctgttca cgctgagcat aggtaaagaa tgcatcaatg atactggcct tgtccggcat
     3421 gtcatcaagg ttagtttgct gaataaagtc aacaatcaga ccttctttgg cacggttgcc
     3481 cagacttgag cggattaagc gtctgacctc ttcagtcagg gcttctttac cttttttctg
     3541 acgattgtgg tcgaaaatca gaccaaggat gtagtccagg ttaatctcct gcgacttcag
     3601 caggtcaatt tcaaacacca catcatccca gtctgtggtt gatttatccc tgtcgtttga
     3661 tgctttttcg cgtcgctgcc agtccctgat atcattgtat gctgagcgat agtcctgaac
     3721 tttacgttct gccgggagac gaatggtttg taattcagca aatttttcat catccacata
     3781 gtgctctgct ttaaaggttt ctaccgcttc cgggtcacta atatcaacct tctgcagggc
     3841 tttcagcgtg gcaaattcat catagttttg caggatgttt tctgcccgca gatattcacc
     3901 aaacagtttg acgaaggctt ttttctcttt ttcactgtcg atactggcag ggtcagggaa
     3961 gcgctgctcc agctccgaaa caacagccat aaaacctcgt ttagcctcac cggttgccgc
     4021 atcggtaaag ccctgcatgt actcttcgta gcttttttcc agcaccacat tcttggtgtt
     4081 tttgtcgcca aaaagagtga tggcatcaat ggtcggacgc tccaggtccc tgaaagtcac
     4141 gatattgccg aaggttttgg ttgcatcata gatacggttg gtgcgtgaga atgcctgcat
     4201 cagaccgtga taacgcaggt ttttgtcaac gaacagagta ttgagcgtcg gagcatcaaa
     4261 gccggttaag aacattccca ccacgatgag aaggtcaata tcctgatttt tcactcgttg
     4321 agctaaatcg cggtagtagt tctggaaacc gttactgtca gtgctgaaat tggttttaaa
     4381 gcaggcgtta tagtcatcaa tggctgcatc cagaaattcc ttcgcactac tgttcatcgc
     4441 gctggtatca aagctttcgt cagtaatatc accaatggcg ctttgctctt catttgcagc
     4501 aaaggagaag atggttgcca cccgaaggca tttatagctg ccagatttct tctctgcttc
     4561 ttcctgtagc attttaaacg tcgtgtaata agctttcgcc gcgtccacgc tgctcactgc
     4621 taacatggcg ttaaagccct tagctcccgg gaaggttcgg tgcgttttct gccggaagtt
     4681 attgagaatg tattgcgtta tttcctggat acgcaggggg tgcaaaaatg cctgttggtt
     4741 ttcagccgcg ctgagttttt tctcatcagt ttccgtttca agagacttaa actgtgggcg
     4801 gacatcgtta tagtccacct taaacttcag cactttttca tcacgaatcg catcagtaat
     4861 gacgtaagaa tgtaattcac gtccgaagac gctggcggtg gtttctgccc ccagtgcatt
     4921 ttccgggaaa ataggcgtac cggtaaaacc aaactgataa aagcgtctga atttcttttt
     4981 gaggtttttc tgggcctcac caaactggct gcggtgacac tcatcaaaga taaacaccac
     5041 ctgctgctga tagacgggca ggtcagcttc ggctttcatc aggttattga gtttctgaat
     5101 ggttgtgacg atgattttgt tatcgtcctt atccaggttt cttctgaggc cagctgtatt
     5161 atccgaaccg ttgacgctgt ccggagagaa gcgctgatac tccttcatgg tctggtaatc
     5221 gaggtctttc ctgtcgacca cgaagaagac tttatcaata aagtccagtt ccgtagccag
     5281 acgtgcggcc ttaaagctgg tcagggtttt tcctgaccct gtggtatgcc agataaaccc
     5341 gccgctttcg ggttttgacc agttcttcgc tttaaatgag ctgttaattt tccagagaat
     5401 gcgctcggtg gcggcaatct gataggggcg catgacgagc agagtctggc tgctgtcaaa
     5461 cacgctgtaa ttaaacagaa cattcagcag ggtatgcttc tggaagaaag tggcggtaaa
     5521 gtccttcagg tcctttatca gggtgttatc cgatttcgcc cagttcatgg tgaaatcaaa
     5581 gctgttttta tcccgcttag tggtactggc aaagtaccgg gtatcggttc cgttagatat
     5641 cacaaacagt tgcaggtatt taaacaggga gttatcgctg ttaaagcttt ccttgctgta
     5701 acggtgtatc tggttaaagg cttcacgaat cgccacgcca cgctttttga gttcgatttg
     5761 caccagtggc aggccgttga ccagaatggt gacgtcataa cggttagcgt gtgaacctgt
     5821 ctgctcaaac tgctgaatga cctgcacctt attgcgcagc aggttctttt tatctatcag
     5881 atagatgttt tccagacgac catcatcaaa agtaaaatca cagatatagt cgatatggat
     5941 tttacgggtt ttgtccaggc tgctgtcact ggggctgtcc agatactgct ctgcgaaacg
     6001 ccgccattca ctttcgttaa acaccacacc attaagacgc tgaagctggt cccgcacatt
     6061 cgccagcatc gcagtcggtg acttaacgga gataaactca tagccctgat taagaaggtc
     6121 ctgaatcagc tcccgctcaa ggtccaattc gctctggtag ctgtcaccgg tatccataac
     6181 tttggtgaat ttatcaagga cgataaagtt attggattca gcgatggtgt gtgtctgttg
     6241 agtcatagcg catcctgtgt gcccgtgtgg caaaaaccgg cagagaaaga gcaaaactac
     6301 cggcgagtaa aggggtgaag catgacctgg acagcagcag ctgaccggga cgttatctca
     6361 tatctgtcag ttgctgaccg catcaggttt cgggaagctg aacagcaaat cgcgatagta
     6421 ttcgtactgc ttctggcgca gctcgatttc gcggggaagc ccttcgctga ttgacgctgt
     6481 aattgtttcg aatttgtcga gaattttcgc gatacgtgtt tgttccttga gggagggaaa
     6541 tggtactttc aaatttttta acagtccacc tgtaataaga ggttgcccag cacctactgc
     6601 gtactgattt aaattcatgt gtcttagttg atgaaatgcg aaacgtacat tccatgagtc
     6661 agaaggatga ataataagag tattatctga aacgtcaaaa tggccggaga cagcattaac
     6721 caatcctgca tatgcaccga cgcgtgcgac taggagcaaa tctcctgagt aagccgcttt
     6781 atcaataaat cctatcaatc ctgttgaacc gtaaacagga aattttccgt cggcatcccg
     6841 agtcttgttg cgtccagatg cgattttcgt tgtgatttct cccaacgtct tccactcaac
     6901 ctcaccctgt tcaaagctca gcaactggtc gcgatagtag ttgtactgtt ttttacgcat
     6961 ggtaagctca gctgtaagct cagctgtaag ctcagctgta agagcagtaa atttatccag
     7021 aatccgaacg atttcagact ggatggcgag ggatttttcc ggattatccg ggcaggggat
     7081 ggggatctta atatttttga caatctgcgc atttatgttt gtctgggacc ctgttccaag
     7141 ggatttgata tatgtgtatt ggctacacaa gaagtgaaat acatatctat aatgagcaac
     7201 ttcttcatta agttgaatat ttgcgcacgc ttgatttgtt gtcattggaa ttttgtttat
     7261 gccgattttc cccacagttg ccccatacat agcaacaatg acacaattct ttggtatcca
     7321 ttttgcacta gagtttttaa ctccagactc agttattttt acctcggtat cccatatatc
     7381 acaaaagttt acttcttgag ttcgcaacca aggaatgtcg ccatcataaa attctgatac
     7441 gccagtttta ggggttcctc cagatgatat ctttatagaa atatcctcaa gggttttcca
     7501 ctcaacctca accccatcca gcaatttttc cagataactc atctcgctca tttctgcacc
     7561 tcgcagcctt caatttcagc cacaatcgca tcaatatctt tgcgcaactg gtctattttg
     7621 ctgaccgtgg ttttcagctc tgcattcaac tcagcaatat tgattatttc gcggttatct
     7681 ttcgcttcca catagctgct cactgacagg ttatagtcat tcgctacaat ggtttcaaac
     7741 gcgacagatt ttgccagatg agcaacatct tccttgctgg caaatacctg cataatctgg
     7801 tcgatatggg catcggtcag gatattgttg ttggtctctt ttttgaatag ttcactggcg
     7861 tcaataaact gaactttggt atcggtttta tgtttagaca acaccagaat attgacggca
     7921 atagtggtgc caaagaacag gttcggagcc agtgaaatca cggtttcgac atagttatta
     7981 tcgaccagat actgacggat tttctgctct gcgccgccac ggtaaaaaat acccgggaag
     8041 cagacaatcg cagcacgacc tttggccgaa agatagttca gcgcatgtaa tacaaacgca
     8101 aagtcagctt tggatttggg ggccagaacg ccagccgggg caaaacgctc atcgttaatc
     8161 agcgtcgggt catcgctgcc aatccatttc accgaatacg gcgggttaga aacgatggca
     8221 tcaaacggtt tttcatctct gaagtgtggc tcagtcagtg tattacccag cttgatatca
     8281 aacttgtcgt agttgatgtt gtgcaaaaac atgttcatac gcgccaggtt ataggtcgta
     8341 tggttgatct cctgaccaaa aaaaccttct tcgatgatgt ggtcatcaaa ctgcttttta
     8401 gcctgcaata acagagaacc ggaacctgct gccgggtcgt agattttgtt aacgtgggtc
     8461 tgcccgtgca tagccagttg tgcaatcagc ttagagacgt gctgcggtgt aaagaactcg
     8521 ccgcctgact taccagcatt cgccgcatag ttagaaatca ggaactcata ggcatcgccg
     8581 aacaggtcaa tctgatgttc gtggaagtca ccaagtttta gcccttcaac ccctttcaga
     8641 accgcagcca ggcgggcatt tttatcttta acggtgttac ccaggcggtt actggtggta
     8701 tcgaaatcag caaacaaacc tttgatgtca gcttctgaag ggtaaccgta agcagagctt
     8761 tcgatagcaa cgaagatact gtttaaatct gcattcagtc tgtcattggt atttgctttc
     8821 gccgctacgt tgcagaaaag ctgacttggg tagatgaagt agcctttagt tttgatggca
     8881 tcgtctttaa tgtcatcagt aattacgctg tcatccagtt tcgcataaca gatactgtca
     8941 tcaccggctt caatatagct ggaaaaattc tcactgatga agcggtagaa aagggcaccg
     9001 agtacatact gtttaaaatc ccatccatct acggagcctc tgacatcatt ggcaatggcc
     9061 cagatttggc ggtgtagctc tgcacgctgc tgaaggcttg tcattttgta tcctgttgta
     9121 tgattcttaa gtactgttat tggcccgatt ttatcgcaat ttcatatgga tgacaggctt
     9181 tgtatgtcat gccttgcact gagagttcgt ttgcccggtt gtgtagccaa cactctttca
     9241 gtcaggccag tagaaacgtt gtgctgacga taaatactga agccccaata ctatcggggc
     9301 tgaacgttca gtcggtgaga atttcttcat ccgtatccat tgtggatggt gatgaatcta
     9361 tttttgttgt aagaaatacc cgattcagta gtctttgctt cagggcgttt cttcgctcat
     9421 tgtagaactc cgggaagcga ctgaactcaa tattttcatc tgaaactaac agcattgatg
     9481 gcgaatagtt gtttgatggc tgagatagcc attgtttcag ggaagtatcc tgtttactta
     9541 tattctgaga gtgattcagt aaatgaagat taggtatggt gtcccagtgc tccggatttt
     9601 cataaaagct gaggt
//
LOCUS       DAPHBJ010000169         9490 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.171, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000169 DAPHBJ010000000
VERSION     DAPHBJ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.171"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            189..1253
                     /gene="ulaG"
                     /locus_tag="RHZ76_003590"
     CDS             189..1253
                     /gene="ulaG"
                     /locus_tag="RHZ76_003590"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDV2814166.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQVDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1361..2116
                     /gene="ulaR"
                     /locus_tag="RHZ76_003591"
     CDS             1361..2116
                     /gene="ulaR"
                     /locus_tag="RHZ76_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HDV2814167.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2113..2862)
                     /gene="yjfP"
                     /locus_tag="RHZ76_003592"
     CDS             complement(2113..2862)
                     /gene="yjfP"
                     /locus_tag="RHZ76_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDV2814168.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCAIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEK
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGSGYFTSLARSLFPPLIPETTA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            3044..3373
                     /gene="bsmA"
                     /locus_tag="RHZ76_003593"
     CDS             3044..3373
                     /gene="bsmA"
                     /locus_tag="RHZ76_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HDV2814169.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIKAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3522..3797
                     /gene="yjfN"
                     /locus_tag="RHZ76_003594"
     CDS             3522..3797
                     /gene="yjfN"
                     /locus_tag="RHZ76_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HDV2814170.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3914..5539)
                     /locus_tag="RHZ76_003595"
     CDS             complement(3914..5539)
                     /locus_tag="RHZ76_003595"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HDV2814171.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(5623..6786)
                     /locus_tag="RHZ76_003596"
     CDS             complement(5623..6786)
                     /locus_tag="RHZ76_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDV2814172.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLIPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPLYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6789..7427)
                     /locus_tag="RHZ76_003597"
     CDS             complement(6789..7427)
                     /locus_tag="RHZ76_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HDV2814173.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRSTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(7437..7835)
                     /locus_tag="RHZ76_003598"
     CDS             complement(7437..7835)
                     /locus_tag="RHZ76_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HDV2814174.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRPYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(7853..8512)
                     /locus_tag="RHZ76_003599"
     CDS             complement(7853..8512)
                     /locus_tag="RHZ76_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HDV2814175.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKAMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(8563..9261)
                     /locus_tag="RHZ76_003600"
     CDS             complement(8563..9261)
                     /locus_tag="RHZ76_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HDV2814176.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(9280..>9490)
                     /locus_tag="RHZ76_003601"
     CDS             complement(9280..>9490)
                     /locus_tag="RHZ76_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HDV2814177.1"
                     /translation="PDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALSLKSSLE
                     DILLEITSLVDNALDLAEITEEYSH"
BASE COUNT         2471 a         2505 c         2249 g         2265 t
ORIGIN      
        1 atttatttaa attcatttag ttaatttaac tacacattcc attaaatctt tccacactca
       61 aaatcaacaa caacttccct ggcaaaaagt caaaaaatgg tcattgcctt cctgaaaaaa
      121 tagtgtcagg attaatcaaa accaatcaca tattgattcg aattgaacta tcatgaaggt
      181 aaatggcgat gagtaaagtg aaaagtatca cccgtgaatc ctggatcctg agcactttcc
      241 cggagtgggg tagctggttg aatgaagaaa ttgaacaaga acaggtcgct cctggcacat
      301 ttgcgatgtg gtggcttggc tgcaccggga tctggttaaa atcggaaggt ggcaccaacg
      361 tttgcgttga tttctggtgc ggcactggca aacaaagtca cggtaacccg ttaatgaaac
      421 agggtcacca aatgcagcgc atggctggcg tgaaaaaact gcagccaaac ctgcgcacca
      481 ccccgtttgt tcttgatccg tttgcgattc gccaggtcga cgcggtactg gcgactcacg
      541 atcacaacga tcatatcgac gtcaacgtcg ctgctgctgt gatgcagaac tgtgctgatg
      601 acgtaccgtt cattggaccg aaaacctgtg tggatttgtg gattggctgg ggcgtaccga
      661 aagagcgttg catcgtggtc aaaccgggcg atgttgtaaa agtgaaagac attgaaattc
      721 atgcgcttga cgctttcgac cgtactgcac tgatcaccct gcctgccgat caaaaagcgg
      781 ctggcgtact gccggacggc atggacgatc gcgcggtgaa ctatctgttc aaaacgcctg
      841 gcggctccct gtatcacagc ggcgactccc actactctaa ctattatgcg aagcacggta
      901 acgaacatca gatcgacgtg gctttaggtt catacggcga aaacccgcgc ggtatcaccg
      961 acaaaatgac cagcgccgat atgctgcgta tgggtgaagc cctgaatgcg aaagtggtta
     1021 tcccgttcca ccacgatatc tggtcaaact tccaggccga tccgcaagag atccgcgtgc
     1081 tgtgggagat gaaaaaagat cgcctgaagt atggcttcaa gccgtttatc tggcaggttg
     1141 gcggcaaatt tacctggccg ttggataaag acaacttcga gtaccactat ccgcgtggtt
     1201 tcgatgattg cttcactatt gaaccggatc tgccgttcaa gtcattcctg taatctgtcg
     1261 tttataccgg atagcgtgga gtagttcgcg ctatccggcg tagtatttcc tggctatttc
     1321 aaatatcatc taaaaaaatc aaattttatc ggaatagctc atgactgaag cacaaagaca
     1381 tcaaatcctc ctggaaatgc tcgcacaatt gggctttgtg accgttgaga aagtcgttga
     1441 gcgtctggga atttcgcctg ccactgcgcg acgcgatatc aataaacttg acgaaagcgg
     1501 caaactgaaa aaagtgcgca atggcgcaga agctattacc caacagcgcc cgcgctggac
     1561 gccgatgaat ctgcatcagg cgcagaatca cgatgaaaaa gtacgtatcg ctaaagcggc
     1621 ctcgcagctg gttaatccgg gcgaaagcgt agtcatcaac tgcggctcca ccgcgtttct
     1681 gcttgggcgg gaaatgtgtg gcaagccagt gcaaatcatc actaattatc taccgctggc
     1741 aaattacctg atcgatcaag aacatgacag cgtgatcatt atgggcggac agtacaacaa
     1801 aagtcagtcc atcactttaa gcccgcaggg cagcgaaaac agtctctatg ccgggcactg
     1861 gatgtttacc agcggcaaag gactgacagc agaagggttg tataaaaccg atatgctgac
     1921 ggcaatggca gagcagaaga tgctgagcgt ggtagggaaa ctggtggtac tggttgatag
     1981 cagtaagatt ggcgaacgcg cgggaatgct ttttagccgt gccgatcaaa ttgatatgct
     2041 gatcaccggc aaaaatgcta acccggaaat cctgcaacaa ctggaagcgc aaggggtcag
     2101 cattctgcgt gtttaaagat gctggcggaa aaatgtcact gcagcatcta acgcctcagg
     2161 cgtaatgcgg tgacgcacgc ctggctgcca tgaacaggtt agcagtttat cccgtcccgt
     2221 ctcgcttaag gcctgctgca aacgtagtga ttcgtcggca ggcacaacgt catcgtcgag
     2281 gccatgccac agcagtagag gtctgtcact aagttgttcc aggtggtttg tcgcttccca
     2341 ctctgccagc ggcgcgacaa tgttattgaa ttcattctgc tgtgctgtcg tttcaggtat
     2401 cagcggtgga aacagtgaac gggcgagtga tgtaaaatag cccgatccca tcatgctggc
     2461 ggtacatctc accgtggggt ggcgagcggt aatccccagt gccgtcatcg cgcccatcga
     2521 cgcaccaccg actgccagac ggtcatcaag cagccacttt tcttcggcta ttgccgcacg
     2581 taaagtagtg aattcctgca tactttgtag caagatttgc cagaattgat ttaaccgccg
     2641 cgctgcgtcg ccactaaaac gactaccgtg atcgggcgca tccggcatga tcacccgcaa
     2701 accagcctgc gccagcgcaa cggcaaaata gctatacacc agactggacg aagtaaagcc
     2761 gtgataaaaa atcgcgcacg gtaacggggt atctttttgt ccgacaggat aagcatgaag
     2821 aacgggaata tctgccagct cgcgtgattc tatttcaatc atctgtctct cctttttgtc
     2881 tggtatgaca tgaagaaatc cttagcacaa gtttctgctg gcggtattag ttcataatgt
     2941 tgagatgtgg gttacgcttt cgtcacgttt tcattcgaaa atcgcgtacc aggtaacaat
     3001 tcgggaacat tcccccaaag ttaaattaat aaggcactac actatggtta gcaggaaacg
     3061 taatagcgtt atttaccggt ttgccagttt attattggtg ttgatgttaa gtgcctgtag
     3121 cgcactgcaa ggtacgccac agccagcacc gccagtaacg gaccatccgc aagagattcg
     3181 ccgcgaccag acgcaagggt tacagcgaat aggtagcgta agcacgatgg ttcggggttc
     3241 tccggatgac gcattagcag aaatcaaagc gaaagctgtc gctgcaaaag ctgattatta
     3301 cgttgtcgtc atggtggacg aaaccatcgt gacaggacag tggtattcac aagccatttt
     3361 gtaccgtaaa taaatcagac aacttacata gtcagcttta cattgctttg cgtccctatg
     3421 cgtttacctg tgcacaataa attaccggct ggaagcctgg gcgtttatta ccgcgatgga
     3481 atgccctgca acatgtgggg aaacgaaaaa tggagctgac gatgaaacaa ttacttgcct
     3541 caccctcgct gcaattagtg acttatcctg cgagcgccac ggcacagtct gcagaattcg
     3601 ccagtgctga ctgcgtgacg ggcctgaatg aaataggtca gatctcagtt agcaatattt
     3661 caggggatcc gcaggacgtg gaacgtatcg tggcgttaaa ggccgatgaa caaggtgcgt
     3721 catggtaccg cattattacc atgtacgaag accagcagcc tgataactgg cgcgtacagg
     3781 cgatactgta tgcatgagtg tcatctacag caaatgaaca atgccggatg cggcgcgagc
     3841 gccttgtccg gcctacaatt ttcacatatt tcatttagtt aatcgaaacc agcgtcgcat
     3901 cagtcgtata cgcttacaca cacactcccc ccgtcgcccg cagcaataaa tcattctgga
     3961 tctgctctga caaccgtacg ccgccgcgcg tatctaacat cacctgacac cacgcctgcg
     4021 ccattggcgg agaagcatat ttcaacattt gcgcaccgca gcccagcagg aatagctgat
     4081 gagtaatctc tcgccccagt tcttcagctg gcttacgcag ctgctgctgt aaacgacgaa
     4141 ccgcgcgatc aaaatagcga tcctgccctt tcacttccac aaatgcttcc gacaataagt
     4201 cgtatacgcc cgcttgctta ttgagaacgc gcaacacatc caggcacata atattgccgg
     4261 aaccttccca aatactgttt accggcatct cccggtaaag ccgcggcagc tcgctctcct
     4321 cgcaataacc aatgccgccc agcacctcca tcgcttcggc cacaaacggc atacctcttt
     4381 tgcagatcac aaattttgcc gcaggcgtaa ataaacgcgc ccacagggct tctttggcat
     4441 cggcacgccg gtcccacgct cgcgcaagac gaaacagcaa cgccgtttgc ccttcaagct
     4501 gaagtgccat gcgacttaag acatgacgca taaggggctg ttggatcaat ggattaccaa
     4561 aaacatggcg ttgatgtgca tgataaatcg ccagcgaaaa tgcacggcgc atcatggcat
     4621 ggctacccag ggcgcaatca aaacgcgtca tcccacccat tttcaggatc agacgaattc
     4681 cttccccttc ctgccccaac aaccaaccaa tggcatcctg aaactccact tcgcaactgg
     4741 cgttagagcg attacccagc ttatctttca gccgctcgag gcgaatcgcg ttgcgttgcc
     4801 cgtcaggcaa aaagcgcggc acaaaaaagc aggacagacc acccgcggtc tgcgccagca
     4861 ccagatgcgc atcgctttgc ggaaccgaga aaaaccattt atgccccacc agccgataag
     4921 agccatcttc cagacgctct gcacgggtgg tgttgctcat aacatcggaa ccgccctgct
     4981 tttccgtcat tcccatgcca atcaacaaac cgcgtttttg cccacctggc aataagtgag
     5041 aatcgtagcg atcgctcaat agcggcgtgg tccagtcctg aaacggcgcg ggtaacatct
     5101 gtaacaacaa tggcgtggcg gcaaaggtca tggttatcgg acataacgac cctgcctcaa
     5161 cctgcgcatg taacataaaa cgcgccgcgc gcgccacaaa tgcgccggag cgagcgtctt
     5221 cttcccaggc aagattgtgc acccgattgg tacatagcgc ctgcatcagc aggtgccagg
     5281 cggggtgaaa acgcacatcg tccagacggc gtccttgcgc atcgtagcgc aataattcag
     5341 gcggattcac attcgccagc cgccccagtt caagggattc agccgttcct aactgctgac
     5401 caatactggc tagaaaatcg ctatcccagc cagcaccttc acgcgttacc gcttcgcaga
     5461 gcgcgccatc agacaggtat aagttgctgt tatttaatgg tataggttga ttaaaaacgg
     5521 tgtgagtttg ccagtgcact gtgtctccct ccatcaatgg cagtcaccat tagtatggtc
     5581 actgccatgg attcatgaca gaaagagatt gctaaaacat tcttatccag caatgtaatg
     5641 cggaacgaaa cgtgaggtgt ctttggtgat cagtgtgtta tcttcacgga tccccattcc
     5701 gcacgcttca tcatcgacaa tccagctacc gatgagtgtg tagctatcgc caaaccgcgg
     5761 cagaggttga aacgcctggt agatcatcgg ttcatcggcg taatcaccat cagcgtggtc
     5821 aacgacgtta ttctggccgt cgaaaatggt gacatttccg ccttcgcgcg agtagagcgg
     5881 tttacgcaca tagctttcgc cagcggcaat ctgcgatttt tcaccctcaa accaggatgc
     5941 aagaaggtta ggatgaccag ggaagaagcg ccaaagcagc gggattagcc ctttattact
     6001 caagatactt ttccataacg gctccaccca ttgctcgcga cgcttgcgca gcagcggacc
     6061 gttatcgtca cgcatcatcc actccagcgg atacagctta aatgcacgct ggatgacatt
     6121 atcatcaaga tcggtcagta cgccgccgac gcccaaaccg agatcttcaa tgtagataaa
     6181 ccgcgactcc tgccctgcct gctgggcgca gtcctgcaag tacagcacgg tactcctgtc
     6241 ttcatcggtg tcctgacagc agcaaaaata aaacggttcc cgactgtaaa gctcgctaaa
     6301 gcgtgaaatc aggcgttcct gaatagcatt gtactgatcg gcatcacgcg gaataatgcc
     6361 gctgcgccgg gcatcctcca gccacagcca ctggaaataa gccgactcgt acaatgaagt
     6421 tggcgtatcg gcgttgtact ccagcagctt caccggcgca ttgccacacc aggcaaaatc
     6481 catccgtcca tacagcgaag gatcgcgggc gcgccagctt tcagcgatca catcccagta
     6541 caacggcggg atcgccagtt gcgtcaggat ctcttcatct tttaccgcgc gatccaccac
     6601 ctcaaggcac atctgatgca gttccgcagt cggtttttcg atctgctctt caatctggcg
     6661 cagagtaaaa cggtaagccc gactctcatc ccaatagatt tcattgtcga tgatatgaaa
     6721 gtcgaaaccg ttgtcagcgg cgatctggtc cagatcccgt cgcacaggaa cgttgtgtct
     6781 cagcatgatt agcctcccca atgcccacgg gcgctggaag aacgaccata gccgccgcga
     6841 gaaacggtag acgctttttt ggtggtaaag ccgcccgaag agtaagactc ttttttgcca
     6901 gaaccggagc gccaagagta atcaccagaa gtgctgcgcc agactgggcg cgaagcaaaa
     6961 gaggaaccgc cgctgttata aacaaacggc tcatcgcgat ctttacgaat aacccggctt
     7021 aacaaaaatc cagaaacgac cgggatccaa ctctgttcaa cattgtcgta gtagcaattt
     7081 tcgtacttag actgacagtt ctgctgagtc atattcttcg gaacatcggc ataaaatgcc
     7141 gttttggcgt tgttccagcc acgcgcgcaa atgtcggcat tattaccgtc atcaatacaa
     7201 tcctgcactg tcgcgtaaaa cgttccgtcg ccgtcgttat cgacatcgct actatcgcta
     7261 caacctttca atacgaaaaa agcggcaccg cccattagcg ccaacgttaa gtatttttgt
     7321 gcgtagcgat tgcgacgcgg ttcaaaagga ttattcggtc taccaatgcg actaatcgct
     7381 ccgtgaccaa ttttactgtt atttcttgat ttgcgttttc tggccatgat cgtccattac
     7441 catgtcatac aagctgcgtt aaaaataccg ccagccagcg cggcggttcc cataaacatt
     7501 cctgctgcgg tattgtgatt aataattttt tcgcttaatg cgggcatata aggtctgaca
     7561 ccagcaaaaa cgagtaactg aatcaccagt gcaatcccgc cccaggcgaa atagtctgga
     7621 atactcaccg cattgattgc cgcactggat aaggggataa cgtaacccaa caatgtaccg
     7681 ctgaacgcca gtgacgccgc ggtattattg tttttgatta actgccattc gttgtgcggt
     7741 gtaattttag aatagataaa cagaaaaata atcaccatag ccacgccaat aaaaaaatag
     7801 gcgctaaagg caagaagtga atccagtata tgcattgtaa acgtccttgt aattaaccaa
     7861 taatatgaag tgatgtcagc gggatatcga cacccaacgc gcgcgaaaat aaccactctg
     7921 gctcgccgag atcgttaaaa gattcctcac catttagcag caaatattcg taagtatctt
     7981 cggttacttg ccgttggtag cccatggtaa aattatggac ctcccatttg gcatggtttt
     8041 gattttctac tttttccagc atgtagacgg gttcgatatt tcccggttct tcgctgttaa
     8101 aaaatcgctg ccagcgtttt tcctgccagt ttaaggtcat tgctcccatc gctttggcgt
     8161 tgatcgcctc gcgccagtga ctctctttac tgataccgta gctctcttca tagacaaaga
     8221 gcttgatatc atcaatgtca tcaatatctt cgccgccggt ggtattgatt tgcagaaact
     8281 catcgcctga agtgtagtag cggaaaatct gactaccgcc gcccagatcg atgtggctga
     8341 cggcggctac cgtaaattcc tcacccggca gcgcaatcag caattcatct tccagcaaac
     8401 gaaacgctaa cgtgtcgagc gtaaagccac tattgagatg aagtcccagc ggaccacgag
     8461 cgatagctgg cttattatcc ttgccaaaca gacgctggaa aaaaccagac atattttatc
     8521 cttaaaatag ttgccagcct tttagcgagg ctggcaaaaa aattactcgc cctgttggcg
     8581 ttgcagtctt gctaatacat cctgggcgct acttttattg ctaccgccaa ttccggcttc
     8641 cgccagcttt tcgtcaaggt cgcgaccgtc tgcgactttc tccaactgtg cggcagcatc
     8701 caggcgagcc tgacgttcgg cctgacgcgt ttgcaggcgt tttaaggatt ctgccgctgt
     8761 cgaaacgctg gaagatgcgc caacggtaga ggttgttaca gcctgttgtg cacgctgcat
     8821 ggcttcagtg gctttaacga cttccatttg ctgctcaaac tgagcgatac gctgcgctgt
     8881 cgctgtaact gctttttcca cgccatcacg ggagacttcc agattcgaca acacttgctc
     8941 ttcagcggta atgagattct caaggcgcgc gatttcttca gcaacttcgt taatcaacga
     9001 cggattaacg ttcttgctta acgcttccag cgcacgggct tccagactgg ctttgcgctc
     9061 acgtaaatct ttcagcttat cgtgacttaa tttcacccgc gccagcagat caacgcgaga
     9121 ttttccggct ttatcgagtt cagctttagc gtcacgaata tgctgttcca gcatgcgcac
     9181 gccctgggtt tcttcaatgg attcttccgc ctgggagata aacgatttcc ccagcgtaaa
     9241 taaacttttt aaaattccca taaatacccg tccttgtaat tagtgtgaat attcttctgt
     9301 aatttcagcc agatccaatg cgttatctac cagcgaggtt atctcaagca ggatatcttc
     9361 aagagaagat ttaagcgata acgccccgaa aacaatgtaa tactcttcct gttgtacgct
     9421 ggagatccct accgatgaca acggcatcat cttctgattt cttaataaga aggtattaaa
     9481 ttcatctgga
//
LOCUS       DAPHBJ010000170         9417 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.172, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000170 DAPHBJ010000000
VERSION     DAPHBJ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.172"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(138..893)
                     /gene="mlaA"
                     /locus_tag="RHZ76_003602"
     CDS             complement(138..893)
                     /gene="mlaA"
                     /locus_tag="RHZ76_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HDV2814178.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            1187..2119
                     /locus_tag="RHZ76_003603"
     CDS             1187..2119
                     /locus_tag="RHZ76_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDV2814179.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLIRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNYKNPA"
     gene            2195..2269
                     /locus_tag="RHZ76_003604"
     tRNA            2195..2269
                     /locus_tag="RHZ76_003604"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2228..2230,aa:Arg,seq:cct)
     gene            2383..3132
                     /locus_tag="RHZ76_003605"
                     /pseudo
     CDS             2383..3132
                     /locus_tag="RHZ76_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311971.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            complement(3128..3514)
                     /locus_tag="RHZ76_003606"
     CDS             complement(3128..3514)
                     /locus_tag="RHZ76_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2814180.1"
                     /translation="MEPLREIRNRLLNGWQLSKMHTFEVAARHQSFALAAEELSLSPS
                     AVSHRINQLEEELGIQLFVRSHRKVELTHEGKRVYWALKSSLDTLNQEILDIKNQELS
                     GTLTLYSRPSIAQCWLVPALGDFTRR"
     gene            3732..5069
                     /gene="dsdX"
                     /locus_tag="RHZ76_003607"
     CDS             3732..5069
                     /gene="dsdX"
                     /locus_tag="RHZ76_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
                     /protein_id="HDV2814181.1"
                     /translation="MHSQIWVVSTLLISIVLIVLTIVKFKFHPFLALLLASFFVGTMM
                     GMGPLDMVNAIESGIGGTLGFLAAVIGLGTILGKMMEVSGAAERIGLTLQRCRWLSVD
                     VIMVLVGLICGITLFVEVGVVLLIPLAFSIAKKTNTSLLKLAIPLCTALMAVHCVVPP
                     HPAALYVANKLGADIGSVIVYGLLVGLMASLIGGPLFLKFLGQRLPFKPVPTEFADLK
                     VRDEKTLPSLGATLFTILLPIALMLVKTIAELNMARESGLYILVEFIGNPITAMFIAV
                     FVAYYVLGIRQHMSMGTMLTHTENGFGSIANILLIIGAGGAFNAILKSSSLADTLAVI
                     LSNMHMHPILLAWLVALILHAAVGSATVAMMGATAIVAPMLPLYPDISPEIIAIAIGS
                     GAIGCTIVTDSLFWLVKQYCGATLNETFKYYTTATFIASVVALAGTFLLSFII"
     gene            5087..6415
                     /gene="dsdA"
                     /locus_tag="RHZ76_003608"
     CDS             5087..6415
                     /gene="dsdA"
                     /locus_tag="RHZ76_003608"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HDV2814182.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(6523..8061)
                     /gene="emrY"
                     /locus_tag="RHZ76_003609"
     CDS             complement(6523..8061)
                     /gene="emrY"
                     /locus_tag="RHZ76_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HDV2814183.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(8061..9224)
                     /gene="emrK"
                     /locus_tag="RHZ76_003610"
     CDS             complement(8061..9224)
                     /gene="emrK"
                     /locus_tag="RHZ76_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HDV2814184.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         2577 a         2176 c         2161 g         2503 t
ORIGIN      
        1 aaatagtcaa tgacgaacat cagtattttt acatcattac gtccgggtca ttgtcggttt
       61 atctcctttt acttgtggta attgagggat aacaacaaaa aaggtgagtt ttgcgactca
      121 ccttttttat ttatttctta ttcagaatcg atatctttta aatcatcctg aatcgcttgc
      181 gcgttcggat tttcctgcgg tttgagttcg ccgccattag cgatgaaatc atgacgctgg
      241 aagtacgctt cgcgcaccat aatataagga tcggacgact gacgcagcag accgtcggaa
      301 tccagcagct gagcgcgggt ttcgatccct tcaagcgtcc atttacccac agacatcggc
      361 caggtcaacc aggaaagaac cgggtaaaga ccatccgcca tatcaccacc gtcatcacgc
      421 agcgtgaagc taccgtagaa tggtaactga acgtacggcc cataacccac gccataatga
      481 ccaagcgtac taccgaagcg gtgaggttca gtccgttgca gtttcgggtt cgccatccct
      541 gcaacatcaa taaagccgcc catccccaaa atggtgttca ggaaaaagcg ggtaaagtgg
      601 accatcccct gataagggtc gccctgcaag aagtagttaa ccatcaccgc aggttcttca
      661 aggttgccgg taaagttgct caaaccgtta cgcgccggtt gcggaacata atcacgccag
      721 gcgacagcga ccggtcgaac aatatacggg tctaatacat tgaagttgaa gttgtacatg
      781 gtgcggttga acccttctaa cgggtcagaa cgcccttgct gatctgtacc ggaactcgca
      841 caccccacca gaagcgtagt tcccagagca agcgccgaca ggcgaagctt cataaatgtc
      901 tccctgtttt tttatggctt atgcagtttg ccatccatga cggaacgata ccgtatccgc
      961 ctgtttaggt gtgggcgatt gtaacagcac gtcaactgat gtccagacgc cctgatttgc
     1021 tgatttgatc atagcctggt aatcgccgcc ctgtaggcta cttgattcta tagaaacaga
     1081 aaaaggcaaa cgcagccttt tctacatttt cagagtaact cccgtcagtt gcgagcaaaa
     1141 aagccgctac gctttagcta tacgtgctaa ttcaagagaa gagaccatgg acaacgacaa
     1201 aattgatcaa cacagcgacg aaattgaagt tgagagcgaa gaaaaagagc gcggcaaaaa
     1261 aatagaaata gatgaagacc gactcccctc ccgggcgatg gcaattcatg agcatatccg
     1321 ccaggatggt gaaaaagagc tggaacgcga cgcaatggcg ctactgtggt cagccattgc
     1381 ggcgggtctg tcgatgggcg cttcgttact ggcaaaaggg atatttcatg tcgaactgga
     1441 aggtgtgccg ggcagcttct tgctggagaa tctcggttat acctttggtt ttattatcgt
     1501 cattatggcc cgccagcaat tatttaccga aaataccgtg actgcggtac tacccgtcat
     1561 gcaaaaaccg acaatgagca acgtcggctt acttatacgg ttatggggcg tcgtgctgct
     1621 gggtaatatt ctcgggacag gtattgcggc gtgggcattt gaatatatgc ctatcttcaa
     1681 tgaagaaact cgcgatgcat ttgtcaaaat cggcatggat gtgatgaaga acacccccag
     1741 cgagatgttt gccaacgcga tcatttccgg ctggctgatc gccactatgg tttggatgtt
     1801 tcctgcagcg ggtgcggcaa agattgtggt gattatattg atgacctggc ttattgccct
     1861 gggtgacacc acccatatcg tggtcggttc tgttgaaatc ctctatctgg tgtttaacgg
     1921 tacgctgcac tggagcgatt tcatctggcc cttcgcacta cctactttag cggggaacat
     1981 ctgcggcggc acctttatct tcgcgttaat gagtcatgca cagattcgta acgacatgag
     2041 caataagcgt aaagcagaag cacgccaaaa agcagaacgt gcggaaaaca ttaagaaaaa
     2101 ttataaaaac ccggcataaa tggcgagggt ttaagcaatc gagcggcagc gtacttaccc
     2161 cgcactccat tagcgggtat actcatgccg cattgtcctc ttagttaaat ggatataacg
     2221 agcccctcct aagggctaat tgcaggttcg attcctgcag gggacaccat cccgccgtac
     2281 actaacgtct accattgtct ataaacccca tgcaaccata taaaaataaa gagattttat
     2341 tctcatgacg tccattgccg tctattgaaa tcaagtaaat ctatggggca taattggggg
     2401 cattgctggt tcgattgtaa atgtgccccc aatatgtcca gaaataccct caacaaatta
     2461 acagaccgtc agtgcaaagc agctaagcca cgagacaaaa cttataaatt gtctgatggt
     2521 ggtggtcttt atcttgaagt atcccagacc gggtcgaagt attggcgaat gaagtatcgc
     2581 cgcccatcag ataagaaaga agacagactt gcttttggtg tatatccaac cattagctta
     2641 caagacgcca gagaaaaacg agacaacgcc aaaaagttgt tagtcaaggg gatagatccc
     2701 aaagcagagc aaagagcagc aaaagcagaa gaaaaaggtg cattcacttt tgagacaata
     2761 ggacgtcaat gggttgaaag ccaccagatg tggaacgaag cccaccgaaa acgcgtactt
     2821 agaagccttg aaatgtatat tttccctcac attggcactt cggatattcg caaactggag
     2881 gcgatgacaa ttttaccttt gtttaagaag gtagatgacg ccgggaaaca cgatacagcc
     2941 aaccgactta aacaacgagt taaggacatc atacatagcg cccgtctgag cggcattaag
     3001 acagaaatca tcacgaatga tctcgatgtc cggttaatgc aatatgaaac taagcatcat
     3061 gccgcactac atcccagaga tttaccagac tttttcactc gattgggcag ctttaaaggt
     3121 aatcctctta ccggcgtgta aagtcaccta atgcgggcac caaccagcat tgggcgatag
     3181 agggccggga atacagagtt aacgttcccg ataactcctg atttttgata tcaagaattt
     3241 cctggttcag ggtatccagc gacgatttta gcgcccaata aacacgtttc ccctcgtgcg
     3301 ttaattccac tttgcgatgg gaacgaacaa acaactgaat gcccaattct tcttccagct
     3361 gattgatacg gtgacttacc gcactggggc tcagcgacaa ttcctctgcc gccagggcga
     3421 aggactgatg cctggcagcc acttcaaaag tatgcatttt tgataattgc cagccgttaa
     3481 gcagccgatt tcttatttca cgaaggggtt ccataatcac ctcatttttc gcttaagtgt
     3541 aaaaaaatag cggcaaaatt tcagctatga gatgagctaa agtgaaccat atctcaattc
     3601 accttcattt ttagatgtaa atcactccat tgatgcaatt tacctcatgt gaaaggcaaa
     3661 ttttatcgtt tgtcagcctg cgttgttttt ttgtccaata tcatcaggtt aatcacaggg
     3721 gaaggtgaga tatgcactct caaatctggg ttgtgagcac gctgcttatc agcatcgtgt
     3781 taattgtact gaccatcgtg aagttcaaat tccacccgtt tctggcgctg ttgctggcca
     3841 gcttcttcgt gggaacgatg atgggcatgg ggccactgga tatggtaaat gctattgaaa
     3901 gtggaattgg cggaacgctg gggttcctcg cagcggttat cggccttggc acgatactgg
     3961 gaaaaatgat ggaagtatcc ggggccgcag aaagaattgg tctgacactt caacgctgcc
     4021 gctggctttc agttgatgtc attatggtgc tggttggcct gatttgtggc atcacgctgt
     4081 ttgttgaagt gggcgtcgtg ctattgattc ctctggcttt ttcaattgcc aaaaaaacca
     4141 atacctcatt attaaagctt gccattccgc tatgtaccgc attgatggca gtgcactgcg
     4201 tggttcctcc acatccggct gctttatatg ttgccaataa gctgggcgca gatatcggtt
     4261 cggtgatcgt ctacggtttg ctggttgggc tgatggcatc actgatcggt ggcccacttt
     4321 tccttaaatt tctgggtcaa cgactgccct ttaaacctgt acccacagag tttgcagatc
     4381 tcaaagttcg cgatgaaaaa acactaccgt cattaggcgc aacgttattc accatactgc
     4441 tacccattgc gctgatgttg gttaaaacga ttgccgaatt gaatatggcg cgtgagagtg
     4501 gtttgtatat cttggttgag tttattggca accctatcac tgccatgttt atcgccgtgt
     4561 ttgtcgccta ttatgtgttg ggtatacgcc agcatatgag catggggacg atgctcacac
     4621 atacggaaaa tggcttcggt tctattgcta atattttgct gattatcggg gccggaggcg
     4681 cattcaacgc cattttaaaa agcagcagtc tcgctgatac gctggcagtt attctctcca
     4741 atatgcatat gcacccgatt cttctggcct ggttagtggc tcttattctg catgcggcag
     4801 tgggctccgc taccgtggca atgatggggg caacggcaat tgttgcaccc atgctgccgc
     4861 tgtatcccga catcagcccg gaaattattg cgattgctat cggttcaggt gcaattggct
     4921 gcactatcgt tacggactcg cttttctggc tagtgaagca atattgcggc gctacgctca
     4981 atgaaacatt taaatactat acgacagcga catttatcgc ttcagtcgtc gctctggcgg
     5041 gcacattcct gctgtcattt atcatctaag cgcaaagaga cgtactatgg aaaacgctaa
     5101 aatgaactcg ctcatcgccc agtatccgtt ggtaaaggat ctggttgctc ttaaagaaac
     5161 cacctggttt aatcctggca cgacctcatt ggctgaaggt ttaccttatg ttggcctgac
     5221 cgaacaggat gttcaggacg cccatgcgcg cttatcccgt tttgcaccct atctggcaaa
     5281 agcatttcct gaaactgctg ccactggggg gattattgaa tcagaactgg ttgccattcc
     5341 agctatgcaa aaacggctgg aaaaagaata tcagcaaccg atcagcgggc aactgttact
     5401 gaaaaaagat agccatttgc ccatttccgg ctccataaaa gcacgcggcg ggatttatga
     5461 agtcctggca cacgcagaaa aactggctct ggaagcgggg ttgctgacgc ttgatgatga
     5521 ctacagcaaa ctgctttctc cggagtttaa acagttcttt agccaataca gcattgctgt
     5581 gggctcaacc ggaaatctgg ggttatcaat cggcattatg agcgcccgca ttggctttaa
     5641 ggtgacagtt catatgtctg ctgatgcccg ggcatggaaa aaagcgaaac tgcgcagcca
     5701 tggcgttacg gtcgtggaat atgagcaaga ttatggtgtt gccgtcgagg aaggacgtaa
     5761 agcagcgcag tctgacccga actgtttctt tattgatgac gaaaattccc gcacgttgtt
     5821 ccttgggtat tccgtcgctg gccagcgtct taaagcgcaa tttgcccagc aaggccgtat
     5881 cgtcgatgct gataaccctc tgtttgtcta tctgccgtgt ggtgttggcg gtggtcctgg
     5941 tggcgtcgca ttcgggctta aactggcgtt tggcgatcat gttcactgct tttttgccga
     6001 accaacgcac tccccttgta tgttgttagg cgtccataca ggattacacg atcagatttc
     6061 tgttcaggat attggtatcg acaaccttac cgcagcggat ggccttgcag ttggtcgcgc
     6121 atcaggcttt gtcgggcggg caatggagcg tctgctggat ggcttctata cccttagcga
     6181 tcaaaccatg tatgacatgc ttggctggct ggcgcaggaa gaaggtattc gtcttgaacc
     6241 ttcggcactg gcgggtatgg ccggacctca gcgcgtgtgt gcatcagtaa gttaccaaca
     6301 gatgcacggt ttcagcgcag aacaactgcg taataccact catctggtgt gggcgacggg
     6361 aggtggaatg gtgccggaag aagagatgaa tcaatatctg gcaaaaggcc gttaataacg
     6421 tttcaacgca gcatcgcaat cctttccctg ggtgagcgat gctgccgatg gcgcagactt
     6481 aagatccccg gtcttacccg ctataacccc ctccttttat catcacccaa cgcctttcgc
     6541 tgtaaacggc ggtttcgcaa accaaaccaa aaccgtaagt aagataaaag caatagccgc
     6601 catacggaaa atctcatttg cagaaataga aagtgactgc tgggttattt cattattaat
     6661 ttcattaaga actcctgaaa gcgaaccata atatttatcc ataatttgtg acgatgaatt
     6721 aaacacgggg ttaaattgat cgatggttgc tgtcaactga ctatggtgta acgattcgcg
     6781 tcgtccccac agcgtcattg tcaacgacgt accaactgat cctgacaagg tacgaaaaaa
     6841 attactcata ctcgaggcat tggcaaattt attatctggc aagcctgaaa acgaaatcgt
     6901 tgttaagggt aaaaagaaac aggcaacggc gaatccctga aaaaactgcg gcaaaatgat
     6961 gcctgtaaaa tcaatcgttg gcataaatgt cacagaacgc cagtaatagc aaaccgcata
     7021 catcaaaaaa ctaaatgtca ctaacaaccg catgtctatt ttgttgccat aacgtcctat
     7081 caaaggtgaa attaatagtg gcatgatgcc gatgggcgca taagcaagtc cggcccatat
     7141 cgcattatac cccatcgttt cctggagtaa ctgcggcata aggacgatcg ctccagagta
     7201 aaataaatac gcgcatgtga tactcacaat accaatggtg aagttacggg acttaaacaa
     7261 actgagatca agaatcgggt tctctgaggt cgactcccaa atgactaaag agatcagaga
     7321 aataactgat actactgtta atattattat tgtactcgag ttgaaccaat ccagatcgcg
     7381 ccctttatca agcataattt gcaagccacc aacaccgagc actaacaggg tcagtcctgg
     7441 tagattcatt ttgaccggtg aagtctcagt ttctcttcct ttaagtaagg ttaagcataa
     7501 tgtcaggacg ataatcccca tagggacatt gattaaaaat atccaacccc agctaaagtt
     7561 atcacaaata taaccgccca atatcggccc acatatcgga gcgataatca cggtcattga
     7621 ccataatgcc agagcaaatg ttcttttttc tggcggataa ttccttaata ataaactctg
     7681 tgacagtgga attaacggcc ccgccattaa cccctgaacg actctaaaaa atatcagcac
     7741 atcaagattg gtcgataggc tacacattaa tgaagacagc gaaaaaaaag tgactgaaag
     7801 taaaaataat cttaattcgc ctattctttg tgccaacctg ccagtaacag ggatcgcaat
     7861 ggcatttgct acaccaaacg aggtgataac ccaggtgcct tcgtctgttg atgctcccag
     7921 aaagccagat attgtcggta ttgcgacgtt agaaatagtg gaatccaaca tttgcataaa
     7981 tgtcgctaat gacaatgcaa tagtgacgca ccataacgtc ccaccggtta atggtgccgg
     8041 agttgattta gtgattgcca ttaaagttgt ccattatgcg aaataatgtt gctaatttct
     8101 ttttctatcg gactggtatc gataactaaa gccttactgg tataagccgg catggaggtc
     8161 acggttgaag ccagctcagg catctcggca atgtcttcgt tcttcgtatc aatagttgct
     8221 gtcatcgata aaccaatacg caaggggtgt tccatgagtt cttttggatc aagagaaact
     8281 tcaaccggta cacgctgaac gattttgatc cagttccctg tcgcattttg tgcaggtaat
     8341 aaggagaacg cattgccggt tcccatattg atccctgtca cccgaccatg aaacacaaca
     8401 ttttcaccat aaagatcgct gataatattg accgattgac caatccgtac atccgtgagt
     8461 tgtgtttctt taaagttggc attaacccac atttgacgtg ccggtacgac agccattaac
     8521 gattgtccgg ggctcactgt ttcgccgacc tgaacacttc tctgggcaat atagccggta
     8581 accggactct taatatccgt acgtttaagc gccaaccagg cttctttagt tgcatccgcc
     8641 gcttcaacga cttgtggctg acggtttaat ggtgtgttca ttactaaagc tttattcgct
     8701 ttataagcct ggatagcggc attcaatgcc gctttgctac ttattaacgt atctttggta
     8761 tgctccagcg tttcttttga aataaccccc tgcttcgcta acggcactcg acggttataa
     8821 tcttctaaag attgttgata ctgaatacgt gctgaagcga cttcggcact gtattgttta
     8881 tcctgtaagt atagtttatt cgtttgccga acaatatttg ccagattatt tttagcttta
     8941 ttgagtgcga tagtggcatc agttttatcc agtgaaacta aaatgtcacc ttgtcgaacg
     9001 tagttcgtat ctttatgatt aacgacagtg acactacctg agacttgtgc agaaattgga
     9061 tctgcattcc ccgtgacata ggcgtcatct gtactaatca tgtcttctaa ttccattgac
     9121 caataggcat aggcacctga aaacgcaata aataaaacta ccgccaataa agaaaagtat
     9181 tttcttctgt tagaatgttt tttatttgaa ttaatctgtt ccactattat ctctcatttc
     9241 tcatagatga aatttatgaa ttgaatgaaa agagcattca ttcttcatat caaacacagt
     9301 gcatgtgttg cacattatcc caggaacgtt ggaggcctaa tttaaatgag gtgcaaaaaa
     9361 aacaacagag gactaaaccg tggcttttgc aatacaattc ttacgcctgt aggatta
//
LOCUS       DAPHBJ010000171         9399 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.173, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000171 DAPHBJ010000000
VERSION     DAPHBJ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.173"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1048)
                     /gene="trhU"
                     /locus_tag="RHZ76_003611"
     CDS             complement(<1..1048)
                     /gene="trhU"
                     /locus_tag="RHZ76_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein TrhU"
                     /protein_id="HDV2814185.1"
                     /translation="MKKLKKLLLEILIAVMLSLSLPAMTMAADTGVPGAMCQSAGVWQ
                     GLIKNICWSCIFPMRIMGIGAAPEGAAPSRPGCYCTDQNGIPEIGWQLSFFQPVKIVE
                     VVKSPWCSPFLEGTMLQKSQFDIGKSNTNQPMTATEAGFYDVHLWEFPIMTMLKLLVI
                     GECTAEPYVDASLTYISEVDPMWESDLLTLVLNPEAVVFANPIASMVCAADCVAVTAG
                     KDNLAAYFCAGCDGNLYPLTGHIYANDDAVRTSSLITQRLLTKLHRQGMLMRTMGADA
                     MCEKTWEYFTPRSQYRLSMLYPTPEANGPDCCHRLGDSVHDWSTLKGGRKKIGIDNYV
                     YMLWRYNDCCVRYIP"
     gene            complement(1193..2701)
                     /gene="trhW"
                     /locus_tag="RHZ76_003612"
     CDS             complement(1193..2701)
                     /gene="trhW"
                     /locus_tag="RHZ76_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010892299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein TrhW"
                     /protein_id="HDV2814186.1"
                     /translation="MRSFELKTTIKPLASCLAFYALISTAVASPQYQQVADDIARQAQ
                     VLGAQIPVPHASEGPLPSGSLDSPDTKKYIRQAEAMKKNGDLSQQTNRGYVPGMNADS
                     VQAVIDHTQAIRAQSNNSEAVNDIIRRRDEIQGNSQLSESALKSVENKPEVMRSQSSN
                     IEKMFGSSGISAADFERKIDSSREEVLSTENGITIFASFSLPDYVLEDLLRTASEHKA
                     RVVFNGLKKGTTRLPETQAAINQLVVKGKFDSPLITIDPDAFNQYQITQVPTIISREQ
                     SRFAKMVGSFNVDFFQRELARKPDQDLFPIAGTTYPVEEKSIIKELEERAQKYDWDGA
                     KKRAVADTWKNQYMVNLPPAQEHKEWLIDPTIRVTQDVKDKQGRVIASAGELINPLAR
                     FPQNLTMIIFDPMNPGQLEWAEKQYRQRLGSGQVMPMFTRIKQENGWDHLNDLREKFN
                     GKVFKVNEQIIARFQIKNTPALISTEKEKFRITQFSEAEIRGIGTKIAAEEN"
     gene            complement(2688..3200)
                     /gene="trhF"
                     /locus_tag="RHZ76_003613"
     CDS             complement(2688..3200)
                     /gene="trhF"
                     /locus_tag="RHZ76_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein TrhF"
                     /protein_id="HDV2814187.1"
                     /translation="MREYIPKKRGLLSFDWYVPINLGILILVMLIFFNRFTFGYGLLN
                     GCLPADFYMIDLKDKSVRSGNIIAFHMPKSVRFIRENEKVIKIVAGVGGDRLRVTLDG
                     VYNGNKFYKANARRISVKYGIPAETIERELTIPEGEVFLIGQTDHSWDSRFWGPVKLT
                     SVIGKTYAIF"
     gene            complement(3475..7773)
                     /gene="rsp"
                     /locus_tag="RHZ76_003614"
     CDS             complement(3475..7773)
                     /gene="rsp"
                     /locus_tag="RHZ76_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein RSP"
                     /protein_id="HDV2814188.1"
                     /translation="MRKSGLCLTLLFSLIASIKSVQAEAIMISGKLQADMPAVTFNPG
                     PGDFVAFVNNNTITASGTGNACNVTVDDRATSSVDNLVCFFEWLPNTSGFTANGFNLT
                     GIPNAAGELKLPYKISYFSGSERQKVEVVNGEYTVNALVPVKPTITGLKSSMNGVVQD
                     GYTLKSYLKDEAIRNVTVSVEPRNYVQYISIGSGSACEVAIGGNGCTIDVGSIKVSDS
                     DLLMGSREITVTANSKNNYFAPPEAQKLTLTWDYRPPVVDHTLWNFTDEAKTISIGGQ
                     EIYTGARTVAVAVKVPQEETEGEWWLPTAMTLTMNPDGVFKPTTKVTLEDGTVIDFKQ
                     SWATPLRRTLQPVSGPQKVGDEYLYIFDLTDLTNGSYAASFTVENNSKNTSTYNEPES
                     KLMLSDNPTLMVLKDGAALAKRAPVYFLNEIIVAAFQGQAGVADIKAVTIDNKLVELT
                     PTNHKGIYYLPVGDDLEVNADHEITVIAENLYGKIVTFNTTFTYQPTGFTLKNFEKDI
                     TLYSRVRQYTDLMSQTAGDKCTLFTTEENALAYLEWYGDKSDITACYPQWNNVPDGLE
                     FYFKGRTPGLTGFFNKTGDNLLDYQVYMINGKGSKAVSSRNRHVLITQLPYNPVLTYK
                     KNKVLTGINDNTALAYTTGGEAARLLAKVVPADVTVYVSQNGGESVKTTFKNRSSNND
                     ATTFVQRLKVASAPLWTKNVFDIAVEYSKEPTMRTVDTLNVYTVPDFNIRAAMEIEDK
                     KTATTMELPLKVSVGRYNNSTRKSSFSRTTMGDWDVTIYAQKSVYGKDPETGRYKTTY
                     ERTPLTEALPVNDAGVVETKIKIENMDLGNVRLVGIAKVRSPFADFELTRETSAVSIR
                     VYKGEELEGNLSKSLIIGRIPLSTLVNFKSASTANSDALAPTEWQESSDNGQTWTMLQ
                     DMTGKRSISIRKTEVGKWLYRAKMTNKFTSKESYSDVLTVVTYKQPKLSIDVAEILEG
                     DDLPVTLLDNDEPIPQGTAEVMWSEDKVNWVQGDTTYNVSAADTLPPTIYARMRYLDS
                     DELAEESSWKETSARLTVAKPKRLSVSVSGISSVEVGSKVTLDGKYTNPNSRFKNGND
                     VIEEWTAPDGQKIRGANLSITLTEQMLDKQGYAAFEYSAWLADSKDSTISTRKVSVKS
                     WVYKFPEMKVSTKLKYTMAPSTLHVALSGIKDGGYLGVTYSREWIYDKDNISVLKDDG
                     DTKEFAIAKPGKYTLVIVFRDNRNNEQRIENTFVVDEQTPMTVEMTPKFSNKFMRAPL
                     DVTLRSNIKLAHSADSIDTVTYKVNGEVVPGSKNYWAQLISGLTEKKYEVTLDVVSKM
                     GQRGTASVDFEVVKNAAPQCALSYTESNMSWSFTNKCIDTDGKLVRYEWYINDELRNV
                     FGSTATLSKNLNRGKQDIRVVAYDDSGDSATQRTTVYGLDEGASKSVDSVETSQ"
     gene            complement(7893..8456)
                     /locus_tag="RHZ76_003615"
     CDS             complement(7893..8456)
                     /locus_tag="RHZ76_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer protein"
                     /protein_id="HDV2814189.1"
                     /translation="MTMSDLSATGAKPQRVRVFSQSVAMAFEVNLDKSRSADNSAFVT
                     ETEKYYLSLQFAPPSDNREYGWNSEGSILMKLSQNEAMALASVFLRIKPTLKFEKRKT
                     THRTHQAYKNINIGPNDRGGLLVSASIVPVKMGTFKPLNYNLPVTQMDCVSTGLFLLG
                     FLTLKMPWVSSESIITALRLSESKSGQ"
     gene            complement(8453..8887)
                     /locus_tag="RHZ76_003616"
     CDS             complement(8453..8887)
                     /locus_tag="RHZ76_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer protein"
                     /protein_id="HDV2814190.1"
                     /translation="MKVNFKKVIPLLMVGLTVAGSYSIFNAKPSKPDLYDFTGKVLKT
                     TSVFQPCDKESTPSLNIQIADNGSVHINGVASKVTFVERVPGNEIAVKCTGLQVKNSR
                     LVHTSSYTMIISEGKGGFVISDLTHTKDNEVMSGTWFFKKRV"
     gene            complement(8877..9329)
                     /locus_tag="RHZ76_003617"
     CDS             complement(8877..9329)
                     /locus_tag="RHZ76_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transfer repressor"
                     /protein_id="HDV2814191.1"
                     /translation="MLSRNSLIHGLRRDQLIEVLSISEFPVVLVENPFIQPKSTGKKP
                     VIFNIDEFHVSIAPISSLKYDWEWAPIDTILIEVVIPPAEADLVSAENDFLRDAGIGH
                     IQCEPGGVSIRRTVTFVGGITADNLLYQLRLMCVSALHLIGEELEDES"
BASE COUNT         2414 a         2283 c         1964 g         2738 t
ORIGIN      
        1 tgggtatata cctgacgcag caatcattat agcgccacag catgtaaaca tagttatcta
       61 tgccgatttt tttccgtcca cctttaagcg ttgaccagtc atggacagaa tcgcctaggc
      121 gatgacagca atccggacca ttcgcctccg gcgttgggta aagcattgaa agtcggtatt
      181 gagatcgagg agtgaagtat tcccacgttt tttcacacat tgcgtcagct cccattgttc
      241 tcataagcat tccctgccta tgtaactttg tcagtagacg ctgagttata agtgagctgg
      301 ttctgacggc atcatcattc gcatagatgt gtccagttaa cgggtaaagg ttcccatcac
      361 acccggcaca aaaatatgca gccaaattat ccttcccggc agtcacggcc acgcagtcag
      421 ctgcacatac cattgaggcg atagggtttg caaataccac agcttcgggg tttagcacga
      481 gcgtcaatag gtcactttcc cacattggat ctacttcgct gatatacgtc agactggcat
      541 caacataagg ttctgctgta cattcaccaa taaccagcaa tttcaacatc gtcatgattg
      601 ggaattccca gagatgtacg tcgtagaatc cggcttctgt tgcggtcata ggctggttag
      661 tattgctctt gccgatgtcg aactgtgatt tttgcagcat ggttccctct aaaaatgggc
      721 tgcaccacgg acttttcacc acttcaacaa tttttactgg ttggaaaaaa cttagctgcc
      781 atccgatttc tggtatccca ttttgatccg tgcaatagca accagggcgt gagggggcgg
      841 caccttcagg ggctgctcca attcccatga tccgcatcgg gaagatacaa ctccagcaga
      901 tgtttttgat taggccctgc catactcccg ctgactggca cattgctccc gggacaccgg
      961 tatctgcggc cattgtcatt gcaggcagcg agagcgacag catgacagcg atcaatattt
     1021 ccaaaagtaa cttttttagc ttcttcaccg gcacgctcac tatttagtaa tgaatgttgt
     1081 cacgctttcc atatacgcaa ccactttgtt aaaacactct ctcatctggt ccgggtaagt
     1141 tagcgtcagc caagtggcca gcagcgcccc aaaaataaat cctattttca tatcaattct
     1201 cctctgccgc gatttttgtt ccaataccac gtatttcagc ttcactgaat tgcgttatac
     1261 ggaatttctc tttttcagtg gagatcagcg ctggcgtgtt ttttatctgg aaacgagcaa
     1321 taatttgctc attaactttg aacaccttcc cattgaattt ttcgcgcaga tcattcaaat
     1381 gatcccatcc attttcctgt ttgatacgcg tgaacatcgg catcacctga ccgctgccaa
     1441 gacgctggcg atattgcttc tctgcccact caagttgccc agggttcatt gggtcaaaaa
     1501 ttatcattgt gaggttctgc gggaacctgg ccagtgggtt aatgagctcg ccagcagaag
     1561 caatgacgcg accctgctta tctttaacgt cctgagtaac cctgattgtc gggtcgatta
     1621 gccattcctt gtgctcttga gcgggaggca gattaaccat gtactggttt ttccaggtat
     1681 cagcgactgc tctcttcttg gcgccgtccc agtcgtattt ctgtgctcgc tcttcgagct
     1741 ctttaataat gctcttctct tcaaccggat aggtcgtacc agcaatcggg aaaagatctt
     1801 ggtcgggctt tctggccagt tcgcgctgga agaaatcaac gttgaaagag ccgaccattt
     1861 ttgcaaaccg ggattgctca cgggagatta ttgtcgggac ctgcgtgatc tggtactggt
     1921 taaatgcgtc cgggtcaatc gtaattagtg gggagtcaaa cttccccttc accaccagct
     1981 gattgatagc tgcctgagtt tcagggaggc gtgtagtgcc tttcttcaga ccattaaaaa
     2041 ccactctcgc cttatgctct gacgcggtac ggagtagatc ttccaggacg taatcaggca
     2101 agctaaatga cgcaaagata gtgataccgt tttctgtcga gagcacctct tcccgactgc
     2161 tgtctatttt acgctcgaag tcagcggcgg agattcctga tgaaccaaac atcttttcga
     2221 tattcgaaga ctgactgcgc ataacttctg gtttattttc gacggatttc agtgcagact
     2281 cgctgagctg tgaatttccc tgaatctcat cacgtctgcg aatgatgtcg ttaactgctt
     2341 ccgagttatt actctgagcg cggatagcct gcgtatggtc tataactgcc tgcacactgt
     2401 cagcattcat acccgggaca taaccgcgat ttgtttgctg actgaggtcc ccgtttttct
     2461 tcatagcttc ggcctggcgg atgtatttct tggtatcagg agaatccaga gaaccagacg
     2521 ggagcggacc ttctgatgcg tgtggtaccg gtatttgtgc gcccagaacc tgagcttgcc
     2581 ttgcaatgtc atcagcaacc tgttgatact gggggctagc cacagcggtg gatattaagg
     2641 cgtaaaatgc cagacaggag gccagaggct taatagttgt ttttaattca aaagatcgca
     2701 tatgttttcc caataactga agtcaactta accggccccc aaaaacgtga gtcccatgag
     2761 tggtccgttt gcccaattaa aaagacctcc ccttccggaa tagttaattc tctttcaatg
     2821 gtctcggcag gtatgccgta tttcacagaa atacgtctgg cattggcttt ataaaatttg
     2881 ttcccattat aaacaccatc caatgtgacc ctcaggcgat ctccgccgac tcctgccaca
     2941 attttaataa ccttctcatt ttcacgtata aaacggactg attttggcat atgaaaagct
     3001 ataatattcc ctgaccttac cgatttatcc ttcagatcga tcatgtaaaa atcagcaggt
     3061 aaacatccat ttaatagtcc atatccaaac gtaaatcgat taaaaaaaat caacataacc
     3121 agtatcagga ttccgagatt tattggcacg taccaatcaa aactcagtaa accacgcttc
     3181 ttaggtatgt attctctcat aacctacttc tctcagaatc agaaagcaaa atggactgtc
     3241 ttttacatta ccttaaaacc tattatccat gttttggtac ttccttattt cagcaaagcc
     3301 agctatttct gattatttac cgcctttttt ttctttcatc ccgtctttga tttgtgattg
     3361 tcctggccgc tgtcttactc atgtaaagta actttcccga cgttacccca tcaaaaagtg
     3421 aaaaacaaaa aagccgggac atttctgccc cggctgttat ttcattagct tgccttactg
     3481 cgaggtttca actgaatcta cggactttga cgcgccctca tccagaccat aaacggtcgt
     3541 acgctgagtt gctgaatccc cactatcatc atacgcgaca actcgtatat cttgcttccc
     3601 acgattaagg tttttggaca gtgtcgcagt gctgccgaaa acattcctta gctcatcatt
     3661 gatataccat tcatatcgaa ccagcttgcc atcagtgtca atacatttgt ttgtgaaact
     3721 ccagctcata ttagactctg tgtagcttaa tgcacattgc ggagccgcgt ttttaacaac
     3781 ctcaaaatca actgatgcgg tacctctttg gcccatttta ctgacaacat ccagcgtgac
     3841 ttcgtatttc ttctcagtaa gaccagaaat taactgagcc caatagtttt tactaccagg
     3901 aacaacctcg ccgtttactt tgtatgtcac agtatcaata ctgtcagcag agtgcgccag
     3961 tttaatgtta gatcttagcg tgacatcaag aggcgccctc atgaacttat tagagaattt
     4021 cggtgtcatt tctacagtca tgggcgtttg ctcatcgact acgaaggtat tttcaatacg
     4081 ctgttcgttg ttacggttat cacggaaaac gattaccaac gtatatttcc ccggcttcgc
     4141 aattgcaaat tctttcgtat caccatcatc ttttaaaact gagatattgt ccttatcgta
     4201 gatccattcc cgagaataag ttaccccaag atagccaccg tcttttattc cgctcagcgc
     4261 aacatgtaag gtcgacgggg ccatggtgta tttaagcttt gtactgactt tcatctcagg
     4321 aaacttatac acccaagatt taacagatac tttccgcgtg gaaatagtac tgtccttact
     4381 gtcagcaagc catgcactgt attcaaatgc cgcatatcct tgcttatcca gcatctgctc
     4441 agtcaaagtt atcgacagat tcgcacctct gattttttgt ccgtcagggg cagtccattc
     4501 ttcaatgacg tcatttccgt tcttgaatcg gctgttcggg ttagtgtatt taccatctaa
     4561 agtaacttta cttcccacct cgacgcttga aataccactt actgaaacag ataggcgctt
     4621 gggtttagcg acggttaacc gggcagacgt ctctttccag gatgactctt cagccaactc
     4681 atcagaatcc aggtaacgca ttcgggcgta aatggtcgga ggtagcgtat cagcggccga
     4741 tacgttatag gtagtgtcac cctgaaccca attcacttta tcctccgacc acatcacttc
     4801 ggccgtaccc tgcggaattg gttcatcgtt atccagcagc gtgacaggca aatcatcgcc
     4861 ttcgagaatt tcagcaacat cgatgctcag cttaggctgt ttgtacgtta caaccgtcag
     4921 aacgtcgcta taagactctt tcgacgtaaa cttgttggtc atttttgccc tgtaaagcca
     4981 tttaccaacc tcagttttcc tgatcgaaat gcttcgcttc cctgtcatgt cctgaagcat
     5041 agtccatgtc tgtccattat cagaagactc ctgccactcg gtaggcgcca gcgcatcgga
     5101 gttagctgta ctggccgatt tgaagttaac aagcgtactg agcgggatac gtccgataat
     5161 cagactcttt gagagattac cttctagctc ttcgccctta taaacacgga tgctaactgc
     5221 actggtttcc cgggtcagtt cgaaatctgc gaaaggcgat cgtactttag caatcccgac
     5281 aagtcgcacg ttcccgaggt ccatattttc tatcttgatc ttggtttcaa ccaccccggc
     5341 atcattcacg ggcagtgctt cggttagggg tgtacgttca tacgtcgttt tatatcggcc
     5401 tgtttctgga tcttttccat aaacactttt ctgagcgtaa atggttacat cccagtcccc
     5461 cattgttgtc cgactaaatg aacttttgcg ggttgaattg ttatagcggc caacagacac
     5521 cttcaatggc aattccatcg tggtagcagt ctttttgtcc tcaatttcca ttgccgctct
     5581 aatgttaaag tcgggtaccg tatacacatt gagcgtatca acggttcgca tagtcggttc
     5641 cttcgagtac tcgacagcta tgtcgaagac attcttcgtc cacaacgggg ctgatgccac
     5701 tttaagacgt tgaacaaacg tcgttgcatc gttatttgat gaacggttct taaacgtcgt
     5761 ttttacgctt tcccccccgt tttgtgaaac atagactgtc acatcagcag gaaccacttt
     5821 tgccaataga cgagctgctt ccccgccagt ggtatacgcc agtgcggtgt tatcgttaat
     5881 ccccgtcaac accttgtttt tcttgtaggt aaggaccggg ttataaggta actgcgtaat
     5941 cagcacatgg cggtttctgg acgatacagc tttagagccc ttgccgttta tcatatagac
     6001 ctgatagtcg agcaggttat cgcctgtttt attgaagaac cctgtcaggc ctggcgtccg
     6061 acctttaaag taaaattcaa gcccgtcagg cacattgttc cactgagggt aacaggcggt
     6121 gatatcagat ttgtcaccat accattcaag gtatgccagt gcgttctctt ctgtagtaaa
     6181 gagagtgcat ttatcaccgg ccgtctgact catgagatcg gtatactgcc gaactcgtga
     6241 gtacaatgtg atgtctttct caaagttttt aagcgtgaat cctgttggtt gatacgtgaa
     6301 agtagtatta aatgtcacaa tcttgccgta caggttttca gctatgaccg tgatttcatg
     6361 gtcagcattc acttctagat catccccaac cggcagataa taaatgcctt tgtggttcgt
     6421 gggggtgagc tcaacaagtt tgttatctat cgtaacagct ttaatatccg cgactccggc
     6481 ctggccctgg aatgcggcaa caataatttc atttaagaaa tacacaggag ctcgtttggc
     6541 cagagctgca ccgtccttaa gcaccatcag tgttgggtta tcagaaagca tcagtttgct
     6601 ttcaggctca ttgtacgtag aggtattctt gctattgttc tctaccgtga acgaggcggc
     6661 ataagaacca ttcgtcaagt ctgttaagtc aaagatatac agatattcat cgccgacttt
     6721 ctgtggcccg ctcacgggct gaagagtacg acgtagcggt gttgcccagg attgcttaaa
     6781 gtcgataacc gtaccatctt ctaaagtaac ttttgttgtc ggcttaaaca ccccatcagg
     6841 attcatcgtt agcgtcatag cggttggtaa ccaccactca ccctcagttt cttcctgagg
     6901 cactttgacg gctacggcca ccgttctagc tcctgtatag atctcctgtc caccaatact
     6961 tattgtttta gcttcatccg tgaaattcca gagagtatgg tcaacaacag gaggacggta
     7021 atcccaggtc agcgttagct tctgtgcttc aggaggggca aagtaattat ttttcgaatt
     7081 ggcagtgacc gtgatttccc gtgaacccat cagcaaatcc gaatcactta ctttgatgct
     7141 tccaacatcg atcgtgcagc cattcccacc aattgcgact tcgcaagctg atccagaccc
     7201 gatagagata tattgcacat aattgcgagg ctctacggag acagtgacat tcctaattgc
     7261 ttcatcctta agataggatt taagagtgta accatcctga accactccat tcatagacga
     7321 tttcaggccc gttatcgtcg gtttaaccgg tacaagagca ttaactgtat actccccatt
     7381 aaccacttct actttctgcc tttctgatcc agaaaaataa gaaattttat acggcagttt
     7441 tagttctcct gccgcattgg gtatccctgt taaattgaat ccgtttgctg taaagccaga
     7501 ggtatttgga agccattcaa aaaagcagac caagttatct acgcttgatg tcgcacgatc
     7561 atcaaccgtc acattgcatg cgttgccagt acctgaagca gtaattgtat tattgttgac
     7621 gaatgcaaca aaatcgcccg ggccgggatt aaacgtgacg gcaggcatat cagcctggag
     7681 ttttccggaa atcataattg cctcagcttg taccgattta atcgaagcaa ttaaagaaaa
     7741 caacagggtt aaacataaac ctgattttct catgacgaac acctgttttt ttaagcagaa
     7801 ttagaatcac tctacgttaa aaaattctga aatcttgttc gtaaagagga aaaaaaacat
     7861 taaatttttg caggaaattg aggtttttta gctcactgac cgctcttaga ctcagatagg
     7921 cgaagggcgg taattatcga ttctgaggat acccaaggca ttttaagtgt caggaacccc
     7981 aacaagaaca agcctgttga aacgcaatcc atttgtgtta cgggaaggtt ataatttaaa
     8041 ggcttaaacg tccccatttt aaccggtaca atgcttgcgc ttaccaaaag gcccccacgg
     8101 tcgttgggtc cgatattgat atttttgtag gcttggtgag tgcgatgcgt ggtcttacgc
     8161 ttttcaaatt ttagtgtcgg cttaattctc agaaaaacag atgccagcgc catggcttcg
     8221 ttttgggata gtttcatgag aatgctgccc tctgaattcc atccatattc cctattgtca
     8281 gacggaggag caaactggag agacaggtaa tacttttcag tctcagttac aaacgccgaa
     8341 ttatcagcag agcgactttt gtcgagattt acttcgaatg ccatcgcgac agattggctg
     8401 aatactctga cccgttgagg cttcgcacca gttgctgaca gatcgctcat tgtcatactc
     8461 tcttcttaaa gaaccatgta ccggacataa cttcattatc cttcgtgtgc gtcaaatccg
     8521 aaataacaaa accacctttt ccttccgaaa ttatcatggt gtaagagctc gtatgcacaa
     8581 ggcgggaatt tttgacttgc agacccgtac acttaactgc aatttcatta cccggaactc
     8641 tctcaacaaa agtaactttt gatgcaacgc cattgatatg tacgcttccg ttatctgcta
     8701 tctggatgtt caacgaaggt gtggattctt tatcacaagg ttggaaaaca gaagtggttt
     8761 taagaacttt gccagtaaaa tcgtacaagt caggcttcga cggttttgcg ttaaagatcg
     8821 agtaagaccc cgccactgtt aaaccaacca ttaaaagggg gattaccttt ttaaaattaa
     8881 ctttcatcct ccagttcctc tccaatcaag tgtaaagcac ttacgcacat aagcctgagt
     8941 tgatacagca aattgtcggc ggtgatcccc ccaacaaatg tcactgtacg gcgtatcgaa
     9001 acgcctccag gctcgcattg aatatggcca atacctgcat cgcgaaggaa atcattctcg
     9061 gcgcttacca ggtctgcttc agcaggcgga atgaccactt cgatgagtat tgtatcaatt
     9121 ggagcccact cccagtcgta cttaagcgat gaaattgggg caatggaaac atgaaattca
     9181 tcaatattaa aaatgactgg ttttttgcct gttgatttag gctgaatgaa gggattctcg
     9241 accaggacaa ctgggaattc agaaatagac agaacttcga ttaactgatc tcgacgtaaa
     9301 ccgtgtatta gtgagttgcg tgataacatt cagtcagcct ttattataat aatcagatag
     9361 ttacacatga tcccgctagt tccttaccat cttcccgct
//
LOCUS       DAPHBJ010000172         9192 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.174, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000172 DAPHBJ010000000
VERSION     DAPHBJ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.174"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            118..1515
                     /gene="ulaA"
                     /locus_tag="RHZ76_003618"
     CDS             118..1515
                     /gene="ulaA"
                     /locus_tag="RHZ76_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDV2814192.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            1531..1836
                     /gene="ulaB"
                     /locus_tag="RHZ76_003619"
     CDS             1531..1836
                     /gene="ulaB"
                     /locus_tag="RHZ76_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HDV2814193.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            1846..2310
                     /gene="ulaC"
                     /locus_tag="RHZ76_003620"
     CDS             1846..2310
                     /gene="ulaC"
                     /locus_tag="RHZ76_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HDV2814194.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2324..2974
                     /gene="ulaD"
                     /locus_tag="RHZ76_003621"
     CDS             2324..2974
                     /gene="ulaD"
                     /locus_tag="RHZ76_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDV2814195.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            2984..3838
                     /gene="ulaE"
                     /locus_tag="RHZ76_003622"
     CDS             2984..3838
                     /gene="ulaE"
                     /locus_tag="RHZ76_003622"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HDV2814196.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            3838..4524
                     /gene="ulaF"
                     /locus_tag="RHZ76_003623"
     CDS             3838..4524
                     /gene="ulaF"
                     /locus_tag="RHZ76_003623"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HDV2814197.1"
                     /translation="MQKLKQQVFEANMELPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKAADMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4653..4928)
                     /locus_tag="RHZ76_003624"
     CDS             complement(4653..4928)
                     /locus_tag="RHZ76_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV2814198.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5255..5650
                     /gene="rpsF"
                     /locus_tag="RHZ76_003625"
     CDS             5255..5650
                     /gene="rpsF"
                     /locus_tag="RHZ76_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDV2814199.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5657..5971
                     /gene="priB"
                     /locus_tag="RHZ76_003626"
     CDS             5657..5971
                     /gene="priB"
                     /locus_tag="RHZ76_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HDV2814200.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            5976..6203
                     /gene="rpsR"
                     /locus_tag="RHZ76_003627"
     CDS             5976..6203
                     /gene="rpsR"
                     /locus_tag="RHZ76_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDV2814201.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6245..6694
                     /gene="rplI"
                     /locus_tag="RHZ76_003628"
     CDS             6245..6694
                     /gene="rplI"
                     /locus_tag="RHZ76_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDV2814202.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(6808..7779)
                     /locus_tag="RHZ76_003629"
     CDS             complement(6808..7779)
                     /locus_tag="RHZ76_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDV2814203.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKSKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(7832..9133)
                     /locus_tag="RHZ76_003630"
     CDS             complement(7832..9133)
                     /locus_tag="RHZ76_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDV2814204.1"
                     /translation="MDYWLPIIVLFGAFFFMLALGVPIVYAIGLSTLASISTQLDFNS
                     ALSVVSQKLASGLDSFTLLAIPFFILSGNIMNHGGIARRLINFARILGGRLPGSLAHC
                     NILANMLFGAISGSAVASAAAMGGVMHPQQVKEGYDPAFSTAVNVASAPTGLLIPPSN
                     TLIVYSLVSGGTSIAALFLAGYVPGILLGLALMVIAGIIAVRRGYPKPERPTLRQAGV
                     AIWMAIPSIFLIILIMGGVLSGIFTPTEASAIAVIYTLFLALVLYREISVKDLPKIFL
                     ESVITTAIVLLLIGSSMGMSWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIG
                     TFMDMTPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVS
                     KLPINKIIKPMLPFYAVMVLVLAMVTYIPQISMALPRALGY"
BASE COUNT         2314 a         2243 c         2402 g         2233 t
ORIGIN      
        1 ggtcgcgtca catttaatca taaataatct tgttgtgatt acttttgaaa attagagtga
       61 gtgcacaaca ttccgggtgt gcggaatacc cggttacctc ttcttcagga gatcgttatg
      121 gagatcctct acaacatctt taccgtgttt tttaaccagg tcatgaccaa tgccccgttg
      181 ttgctgggta ttgtgacctg tctgggctac atcctactgc gcaaaagtgt cagcgttatt
      241 attaaaggca cgattaaaac cataattggt ttcatgttgt tgcaggcagg gtccggcatc
      301 ctcaccagca ccttcaaacc ggtggtggcg aaaatgtccg aagtctacgg cattaacggc
      361 gcaatttccg atacttacgc gtcgatgatg gcaaccatcg accgtatggg cgatgcctat
      421 agctgggtag gttacgcggt gttgttagcg ctggcgctga acatctgtta cgtgctgctg
      481 cgtcgcatta ccggtattcg taccatcatg ctgactggtc atatcatgtt ccagcaggcg
      541 gggctgattg ccgttacgtt gtttattttc ggctactcca tgtggacaac cattatctgc
      601 acggcgattc tggtttcgct ctactggggc attacctcca acatgatgta caagccgact
      661 caggaagtga cggacggctg cggtttctcc atcggtcacc agcagcagtt tgcatcatgg
      721 attgcctata aagttgcgcc gttcctcggc aaaaaagagg aaagcgttga agacctcaaa
      781 ctgccaggct ggctgaatat tttccacgac aacatcgtct ccacggcgat tgtcatgacc
      841 atcttctttg gtgccattct gctctccttc ggtatcgaca ccgtgcaggc gatggcaggc
      901 aaagtgaact ggacggtgta catcctgcaa actggtttct cctttgcggt ggcgatcttc
      961 atcatcactc agggcgtgcg catgtttgtg gcggaactct ctgaagcatt taacggcatt
     1021 tcccagcgcc tgatcccagg tgcggttctg gcgattgact gtgcggctat ttatagcttc
     1081 gcgccgaacg ccgtggtctg gggctttatg tggggcacta tcggtcagct gattgcggtt
     1141 ggcatcctgg tcgcctgcgg ttcctcgatc ctgattattc ctggctttat cccgatgttc
     1201 ttctccaacg ccaccatcgg cgtgttcgct aaccacttcg gcggctggcg tgcggcgctg
     1261 aagatttgtc tggtgatggg gatgatcgaa atctttggtt gcgtctgggc ggtgaaactt
     1321 accggtatga gtgcctggat gggtatggcg gactggtcga ttctggcacc gccgatgatg
     1381 cagggcttct tctccatcgg tatcgccttt atggccgtca tcattgtaat tgcactggct
     1441 tatatgttct tcgctggccg cgcactgcgc gcagaagaag atgcagaaaa acaactggca
     1501 gaacagtctg cttaataagg agttttgatt atgaccgtac gtattctggc tgtgtgtggc
     1561 aacggacaag gcagttccat gatcatgaag atgaaagtgg accagttttt aacccaatca
     1621 aacattgacc acacggtaaa cagctgcgcg gttggcgaat acaaaagcga gttgagcggc
     1681 gcggatatca tcatcgcttc tacgcacatt gcgggcgaaa tcaccgtgac cggcaacaaa
     1741 tacgtggttg gtgtgcgcaa catgctctct cctgccgact ttggcccgaa actgctggaa
     1801 gtgatcaaag agcatttccc gcaggatgtg aagtaaggac gcgccatgaa attacgtgat
     1861 tcgctggcgg agaataaatc catccgcctg caggctgaag ctgagacatg gcaggacgca
     1921 gtaaaaatcg gcgttgacct gctggtggcg gcggatgttg tcgagccgcg ttactaccag
     1981 gcgattctgg atgcggttga acagcacggt ccgtactttg tgctcgcacc aggcctggca
     2041 atgccgcacg gtcgcccgga agagggcgtt aagaaaaccg gttttgcgct ggtgacgctg
     2101 aaaaagcctc tggagttcaa ccacgaagat aacgacccgg tggatatcct catcaccatg
     2161 gcggcggtcg atgccaacac gcaccaggaa gtgggcatca tgcagatcgt caacctgttt
     2221 gaagatgaag aaaatttcga ccgtttacgt gcctgccgta ccgagcagga agtactggat
     2281 ctcattgacc gcaccaacgc ggcagcttaa gaaggaattg aagatgtcat taccgatgtt
     2341 acaagtcgcg ctggacaacc agactatgga tagcgcctac gaaaccactc gcctgattgc
     2401 cgaagaagtc gacattatcg aagtgggtac cattctgtgc gtgggcgaag gcgtgcgtgc
     2461 ggttcgcgat ctgaaagcgc tttacccgca caaaatcgta ctggcagacg ccaaaattgc
     2521 cgatgcgggc aaaatcctct ctcgtatgtg cttcgaagcc aacgctgact gggtgacggt
     2581 aatttgctgt gcggatatca acaccgccaa aggcgcgctg gacgtagcaa aagagtttaa
     2641 cggcgacgtg cagatcgaac tgaccggcta ctggacctgg gaacaggcgc agcagtggcg
     2701 tgacgcaggc attcagcagg tggtttatca ccgcagccgt gacgcgcagg ccgcaggcgt
     2761 agcgtggggc gaagcggaca ttaccgcgat caaacgtctt tccgatatgg gcttcaaagt
     2821 caccgtcacc ggtggcctgg cgctggaaga tctgccgctg ttcaaaggta ttccgattca
     2881 tgtgtttatc gctggtcgca gcatccgtga tgccgcttct ccggttgaag ctgcacgtca
     2941 gttcaaacgt tccatcgccg aactgtgggg ctaaggagcg gatatgttgt ccaaacaaat
     3001 cccgcttggc atctatgaaa aagcgctccc cgccggggag tgctggctgg aacgcctgca
     3061 actggcaaaa acgttgggct tcgattttgt cgagatgtcg gtagatgaaa cggacgagcg
     3121 cctgtcgcgc ctcgactgga gccgcgagca gcgtctggcg ctggtgaacg cgattgttga
     3181 aaccggcgtg cgcgtgccgt ccatgtgcct ttctgctcat cgtcgtttcc cgctgggcag
     3241 tgaagatgac gcggtgcggg cgcaggggct ggagattatg cgtaaagcta tccagttcgc
     3301 ccaggatgtc ggtattcgcg tgatccaact ggcgggctat gacgtttact atcaggaagc
     3361 caataacgaa acgcgtcgtc gtttccgtga cggcctgaaa gagagcgttg agatggcaag
     3421 ccgcgcgcag gtgacgctgg cgatggagat catggattac ccattgatga actccatcag
     3481 caaggcgctg ggctacgcgc actatctcaa caacccgtgg ttccagctct acccggatat
     3541 cggcaacctc tcggcgtggg acaacgacgt gcagatggag ttgcaggccg gaatcgggca
     3601 tatcgtcgcg gtacatgtga aagacaccaa accgggcgtc ttcaaaaacg tgccgtttgg
     3661 cgaaggcgtg gtggatttcg aacgttgttt cgaaacgctc aaacagagtg gctattgcgg
     3721 gccgtacctg attgagatgt ggagcgaaac ggcggaagac ccggcggcag aagtggcgaa
     3781 agcgcgtgat tgggtgaaag cgcgcatggc gaaagcgggc atggtggagg cggcataatg
     3841 caaaagctaa aacagcaggt atttgaagcc aacatggagc tgccgcgcta cgggctggtg
     3901 acctttacct ggggcaacgt cagcgctatc gaccgcgaac gcgggctggt ggtgatcaag
     3961 cccagcggcg ttgcctacga aaccatgaaa gcggccgata tggtggtggt tgatatgagc
     4021 ggcaaggtgg tggaagggga gtatcgccca tcttccgaca ccgcgacgca tctcgaactc
     4081 taccgtcgtt acccgtcgct tggcggcatt gtccataccc actccactca tgccaccgca
     4141 tgggcgcagg cggggctggc gatcccggcg ttaggcacca cgcacgctga ctacttcttt
     4201 ggtgatatcc cgtgcacgcg cggattaagc gaagaagagg tgcagggtga gtacgaactg
     4261 aacactggca aagtgattat cgaaacgctg ggcaacgccg agccgctgca tacgccggga
     4321 attgtggtgt atcagcacgg accgttcgcc tgggggaaag atgcccacga cgcggtgcat
     4381 aacgcggtag tgatggaaga agtggcgaaa atggcgtgga ttgcgcgcag tattaatcca
     4441 caactcaatc acatcgacag cttcctgatg aataaacact tcatgcgtaa acacggtcct
     4501 aacgcttatt acgggcagaa gtagaacacg cgctgcggaa atttccttcc tcgggagata
     4561 actggtctaa ttccgcagcc gtttttcaaa aaaagccccc tgcgaagggg gcaaagcaaa
     4621 ctatggcaat gtttcgttgg ttatacctgg tgctaacgat aaatatccgc gctggcgtgc
     4681 atattgccgt tactccccgg ttcccgcatc agaattacgt ggtagtacgt tgcgccttgc
     4741 gcatctgttt cttcatttaa tgcctgacgt gcttcgcttt cagtggcgaa attatgattg
     4801 atataaatca cgcctaagct ttgcacatcg tccatatttc tggcctggtg gttattaatt
     4861 tcaatggctg cccatgtatt tgcacttagc aaaagcacag ccagaagggc taaaactcga
     4921 ctgaacatag atacctcctc gacggctgac tttgtgtgct ctcctctgtg atgatcttct
     4981 gatttaattt taatcaatga taaagaagtt gatggtgacc atttctgatg cagttgttcg
     5041 aaaaaacact gtgacggaat gtgatgaact tcaaatcagg gagttagttc ttttgagcga
     5101 aagatgagat ttatttgtcg gctggccttg agtttagcgg ggcatacaag tactataacg
     5161 cgtcattttt cagccgacct ttaacacgtt ccttgcctcc ccgggattcg gctgacccag
     5221 acaggaggct gaataatccg taaggagcaa ttcgatgcgt cattacgaaa tcgtttttat
     5281 ggtccatcct gaccagagcg aacaggttcc gggcatgatc gagcgctaca ctgctgccat
     5341 cactggtgca gaaggcaaga tccaccgtct ggaagactgg ggccgccgtc agctggctta
     5401 cccgatcaac aaactgcaca aagcacacta cgttctgatg aacgttgaag ctccgcagga
     5461 agtgatcgat gagctggaaa ctaccttccg cttcaacgat gccgttatcc gcagcatggt
     5521 tatgcgtacc aagcacgctg ttaccgaagc atctccgatg gttaaagcga aagacgagcg
     5581 ccgtgagcgt cgcgatgatt tcgcaaacga aaccgctgat gatgctgaag ctggggattc
     5641 tgaagagtaa tttctgatga ccaaccgtct ggtgttgtcc ggcaccgtgt gcaggactcc
     5701 ccttcgaaag gtcagtccat caggaattcc tcactgccag ttcgtgcttg agcatcgttc
     5761 tgtgcaggag gaagccggtt ttcaccggca ggcatggtgt caaatgcccg ttattgttag
     5821 cggacacgaa aaccaggcca ttactcacag tataacggtc ggcagtcgca taaccgttca
     5881 ggggttcatt tcatgccaca aggcaaagaa cggactgagc aaaatggttt tgcatgccga
     5941 gcagattgaa ttgatagatt ctggagacta gccatatggc acgttatttc cgtcgtcgca
     6001 agttctgccg tttcaccgcg gaaggcgttc aagagatcga ctataaagat atcgctacgc
     6061 tgaaaaacta catcaccgaa agcggtaaga ttgtcccaag ccgtatcacc ggtacccgtg
     6121 caaaatacca gcgtcagctg gctcgcgcta tcaaacgcgc tcgctacctg tccctgctgc
     6181 cgtacactga tcgccatcag taatcggtca cggtccatta atacgacttt gagaggataa
     6241 ggtaatgcaa gttattctgc ttgataaagt agcaaacctg ggtagcctgg gtgatcaggt
     6301 aaacgttaaa gcgggctatg ctcgtaactt cctggtaccg cagggtaaag ctgttccagc
     6361 taccaagaaa aacattgaat tcttcgaagc acgtcgcgct gaactggaag ctaaactggc
     6421 tgaagttctg gcagctgcta atgctcgcgc tgagaaaatc aatgcactgg aaactgttac
     6481 catcgcgtct aaagctggcg acgaaggtaa actgttcggt tccatcggta ctcgcgacat
     6541 cgctgacgct gtaactgcag ctggcgttga agtggctaag agcgaagttc gtctgccgaa
     6601 cggcgttctg cgtaccactg gcgaacacga agtgagcttc caggttcaca gcgaagtatt
     6661 cgcgaaagtg atcgtaaacg tagtagctga ataattcgtt attcaacgag acgtaaaaaa
     6721 acgccgacca ttggtcggcg ttttgctttt gtagcgagca cttgcagata tcgataaggg
     6781 ccacaataag cagccctacg aagataatta cttagccgcc tgaatatcag ccagcattgg
     6841 ggcaagggct gggttatcct tagcgaagtt tgcgtacatt ggctgtactt tctcgataaa
     6901 cggagcttta tctacctggt agactttcac gcccatttta tccagttttg caaattcttg
     6961 tttctcttcc tcgctccaca agtctttcat gtagctcatt gattctttag ctgcttttaa
     7021 tataactgcc tgattttccg ggctaagttt atcaaacgca gcattgctca tcagaaccac
     7081 atcgggaacc atagtgtgtt cgtcaaggct gaaggatttt gccacttcac cgtgacgcat
     7141 ggtggtcagc gccatcacgc tgttttctgc catatcgacc acaccctgct gtaaggctgt
     7201 atacaactcg ccgtaatcca gtggcgtcgg gttaccgccc atgactttga tcatttcaac
     7261 tgcgccaggg cttggctgga cacggacttt catgcctttc agatcgtctg gtttcagtac
     7321 tggtttattg ccatagaaac tgcgggcgcc tccatcataa aaagtcagcc cgaaataacc
     7381 tttgcttttc gttgagtcga ggatttttct cccgatatcg ccctgcatga cctgataata
     7441 atgatcacga tcgcggaaca aataaggcag gctaaataat ttataggaat tttcaaatgc
     7501 ttccatttct gccgcattgg tttttaccag tggaatagcg ccagaacgaa tcagctccat
     7561 tgtttctcgt tgagtaccca atgttccatt tggataaata cgaatagtaa tatcaccgtt
     7621 agagtactct ttgctctttt tcgcaaagaa ctccatcgct ttatgaacag gatgagactt
     7681 atcctgatta tgactcagtt ttaatgtcac tgcccaggac tggcttgcgg ataacataag
     7741 aacagcaccg ataatgccag cggatacttt tattttcata gatgttcctt ttctaataaa
     7801 tatggcggca ggtgccgcca gtgaatgcgc atcagtaacc cagtgctctt ggcaatgcca
     7861 tactgatttg cggaatatag gtcaccattg ccagcaccaa taccatcacg gcatagaatg
     7921 gcagcatagg tttgataatt ttattaatcg gtaatttaga tacactacaa ccgacaaata
     7981 acacactgcc tacaggcgga gtacaaatac cgatacacat gttcagtacc atgacaatac
     8041 caaagtggat cgggtccatg cccagttcgg tgacaaccgg taaaaatatc ggcgtaaaaa
     8101 tcagtaccgc cggtgtcata tccataaaag tgccgataat caacaggata atattgataa
     8161 taagcaggat tattattggg ttgtcagaaa tagtgttaag caaatcgaga atcaggaagg
     8221 gaacgtcagc gtttgacatt gcccatgaca ttcccatcga agaaccaatt aataacaaga
     8281 caattgcagt ggtaataacg gattccagaa atattttagg taaatcttta actgagattt
     8341 cacgataaag aaccagtgca aggaaaaggg tataaattac tgcaattgcg gaggcctctg
     8401 tcggggtaaa aataccactt aatacacccc ccataattaa aataatcagg aaaatactgg
     8461 ggatagccat ccatatcgca acgccagcct ggcgtaaagt cgggcgttct ggctttggat
     8521 aaccgcgacg taccgcgata ataccggcta ttaccatcag tgccagaccc agcaaaattc
     8581 ccggaacata acccgccagg aacagtgcgg caatagaagt accaccactg accagtgaat
     8641 agacgattaa cgtattactt ggtggaataa gcaaacccgt cggggcagag gcaacgttaa
     8701 ccgcagtact gaatgccggg tcgtagccct ctttaacctg ttgcggatgc atgacgccgc
     8761 ccatcgcagc cgccgaagcc acggctgaac cagaaattgc cccgaacagc atattggcga
     8821 gaatattaca gtgggcaagt gagcctggca gacgaccgcc gagaatacgg gcaaagttga
     8881 ttaatcgtcg cgctattcct ccatgattca taatgttgcc agacaagata aaaaatggga
     8941 ttgccaacaa cgtaaaacta tctaacccgg aggctaactt ttgggaaacc acactcaatg
     9001 ctgagttgaa atcgagttgt gtggaaattg atgccagtgt cgataaacca atggcataaa
     9061 caataggtac acctaatgcc aacataaaaa agaaggcacc aaataacaca ataatcggta
     9121 accagtaatc cataatttct ccattcctac tatggttggt tgttgtttgt tatctttaga
     9181 gtggttgttt aa
//
LOCUS       DAPHBJ010000173         9060 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.175, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000173 DAPHBJ010000000
VERSION     DAPHBJ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.175"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..243
                     /gene="yeaR"
                     /locus_tag="RHZ76_003631"
     CDS             <1..243
                     /gene="yeaR"
                     /locus_tag="RHZ76_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HDV2814205.1"
                     /translation="YPRLSVMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWH
                     NIEAMTDDTYFNIDFFVAPEVLMEGAQQRKVIHNGK"
     gene            247..429
                     /locus_tag="RHZ76_003632"
     CDS             247..429
                     /locus_tag="RHZ76_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HDV2814206.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            577..825
                     /locus_tag="RHZ76_003633"
     CDS             577..825
                     /locus_tag="RHZ76_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDV2814207.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(884..958)
                     /gene="yoaJ"
                     /locus_tag="RHZ76_003634"
     CDS             complement(884..958)
                     /gene="yoaJ"
                     /locus_tag="RHZ76_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HDV2814208.1"
                     /translation="MKKTTIIMIGVAIIVVLGTELGWW"
     gene            complement(961..1059)
                     /locus_tag="RHZ76_003635"
     CDS             complement(961..1059)
                     /locus_tag="RHZ76_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HDV2814209.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            complement(1064..2116)
                     /gene="dgcP"
                     /locus_tag="RHZ76_003636"
     CDS             complement(1064..2116)
                     /gene="dgcP"
                     /locus_tag="RHZ76_003636"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HDV2814210.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLIGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKTNRKHLLS
                     RIQRYIPEAYSHPFDW"
     gene            2299..2553
                     /gene="yoaF"
                     /locus_tag="RHZ76_003637"
     CDS             2299..2553
                     /gene="yoaF"
                     /locus_tag="RHZ76_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HDV2814211.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            complement(2575..2922)
                     /locus_tag="RHZ76_003638"
     CDS             complement(2575..2922)
                     /locus_tag="RHZ76_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDV2814212.1"
                     /translation="MNIQCKRVYDPAEQSDGYRILVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(2977..4158)
                     /gene="nimT"
                     /locus_tag="RHZ76_003639"
     CDS             complement(2977..4158)
                     /gene="nimT"
                     /locus_tag="RHZ76_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HDV2814213.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            4255..5076
                     /gene="nimR"
                     /locus_tag="RHZ76_003640"
     CDS             4255..5076
                     /gene="nimR"
                     /locus_tag="RHZ76_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HDV2814214.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            complement(5033..5479)
                     /locus_tag="RHZ76_003641"
     CDS             complement(5033..5479)
                     /locus_tag="RHZ76_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDV2814215.1"
                     /translation="MFDVTLLILLGLAALGFISHNNTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            5649..5753
                     /locus_tag="RHZ76_003642"
     CDS             5649..5753
                     /locus_tag="RHZ76_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814216.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            complement(5754..6257)
                     /gene="yeaK"
                     /locus_tag="RHZ76_003643"
     CDS             complement(5754..6257)
                     /gene="yeaK"
                     /locus_tag="RHZ76_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HDV2814217.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(6300..7790)
                     /locus_tag="RHZ76_003644"
     CDS             complement(6300..7790)
                     /locus_tag="RHZ76_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDV2814218.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQAGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            complement(7971..>9060)
                     /locus_tag="RHZ76_003645"
     CDS             complement(7971..>9060)
                     /locus_tag="RHZ76_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDV2814219.1"
                     /translation="SGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICL
                     TSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPD
                     NHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDE
                     YNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVE
                     SLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLE
                     GEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRN
                     ISLVMTEADKALREAKSEGGNKVIIHHI"
BASE COUNT         2502 a         2119 c         2179 g         2260 t
ORIGIN      
        1 tacccacgcc tttccgttat gcatggggcg gtcaaatatc tcggctacgc tgatgaacac
       61 agtgcagagc ctgatcaggt gatccttatc gaagcggggc agtttgcggt gttccctcca
      121 gaaaagtggc acaacattga agccatgact gacgatactt atttcaacat tgacttcttc
      181 gtggctcctg aagtcctgat ggaaggtgcg caacaacgga aagtcattca taacgggaaa
      241 tgaatcatgg gcaaagcaac gtataccgtg accgtcacca ataacagcaa tggcgtttct
      301 gtcgattatg aaacagagac gccgatgact ttgctggtgc cagaagtggc ggctgaagtg
      361 ataaaagatc tggtgaatac cgtacgttct tatgacacgg aaaacgaaca tgatgtttgt
      421 ggttggtaat tatcactgat cgttgatggc gcaatttctg cgccatcgca aaaaaaacct
      481 ccgtgatgct cgtcacaaat cttgccctcc cgatgttgtg ctatgctttt tatcagcgac
      541 taacccgcta atgcaaaaca gataagtgag gaaacaatgg gcattctgtc atggattatt
      601 tttgggctta ttgccggtat tctggcgaag tggatcatgc caggtaaaga tggaggtgga
      661 ttctttatga ctatcctgct ggggatagtc ggtgccgtag tcggcggatg gatcagcacg
      721 ctgtttggct ttggtaaagt cgatggcttc aattttggca gcttcgtggt tgccgttatt
      781 ggtgcgattg tcgtgctatt tatctacagg aagattaaaa gttaacgctt aaattgcaca
      841 aaggctgcac acaggcagcc tttgctattt tttagaggtg acgttaccac catcccagct
      901 cagtgccgag tacgacaata atcgccacac ctatcataat aatcgttgtt tttttcatct
      961 ttatgctccc ggggcagcat agccgccaat aaaaaaccat gctaaaaata cgaccgctgt
     1021 aatgaagatt acaaccggaa aaataatacc gattcgcatg ttatcaccaa tcaaaaggat
     1081 gtgaatacgc ctcaggaatg tagcgctgga tgcgcgacaa aaggtgtttt ctgtttgttt
     1141 ttttctcctg atacatcgca atatcggcag catgcagggc actgtctgca tcggttgttt
     1201 caggatcgac ttctactatg ccaagactgg caccgggata atacaaatca acatcaccta
     1261 agtgatattc tccacgtatt tgctgctgaa tgcgttctcg cagcgacgaa atatccgcat
     1321 tctcgttgtt tagtgaaaca actaaaaact catcaccgcc gagacgacca ataacttcgc
     1381 cattttgctg gagcgtatta aggcgctcgc caacctgaat gagaaacaga tcgccactat
     1441 tatgaccaaa acgatcattg attaatttga agttatccag atcgataaac gcgatcatta
     1501 tcttatggtt aagatgccgg gcgagggaaa acagtgtcgt cagattttca aaaatcgccc
     1561 gccgattcgg tagcccgatt aacgagtcgg tatacgattg cgcaatcagc gcagcattgg
     1621 cttcgcgcag ctgttcaacc agtgcctctt tttgaatata ttgtgcaatc agtccggcga
     1681 ataactgtaa aacctgttcc gcgcgttcac tccactggcg cttctcactg ctggcggcgc
     1741 aaagcgtgcc atagaatgat ccatccggta agtgaattgg cgtgctcaga aatgtggtga
     1801 tgccaagatt gcgtgccgca atacagtcac cccaacggtc ggggacttca tcgctgaaaa
     1861 agcagttttc atcaatggcg cgtttgcata acgagtaatc ccacgagacg gtaaaattct
     1921 ccgggatgtg cattttctgg ctgttacggg caaacataat atgctgcagg cgcgcttcga
     1981 catccacttt ggtcaggtag gttgattcca tatcagtgac catttccagc atctccagaa
     2041 gctgtcggac cagactttcc agtgactgtt ctttggcaag ggattgcgag acgcgggcga
     2101 taatctgatc tgacacggaa caactccagg atacggcggg taaatgctga ttatttaatc
     2161 tttgcaggca tgataaaaca tcatcaacaa agatgaaaca agacgtaata tgttttgagt
     2221 gaatgtaata aaaatagtgc tgcaaaacaa aacgataccg cgatactcta atcttggata
     2281 aatatgtgag atcaataaat gaaaattatc agttttgttc tgccttgctt gctggtcctg
     2341 gctggttgtt caaccccttc tcagccagaa gcacctaaac cgccgcagat tggtatggca
     2401 aatccggcgt cggtctattg ccagcagaag ggcgggacgc tcattccagt gcagacagcg
     2461 caaggggtca gcaacaattg caaattaccg ggcggtgaaa cgattgatga atgggcattg
     2521 tggcggcgtg accatccggc tggtgaaaaa taaccgccgg atgctgtact aaaatcacaa
     2581 gctacgtagc cagtcggcca gcaccagcgc atggttctgc gtggtgtttt ttgctgagta
     2641 gagcagggtc agcggctgtt ttttggcgat gtccgccagc cgctttcctt cttgctcgtg
     2701 ttgcgccagt tctgcaagat attgctcgcg aaaggttgca aaatcgacga cttcgccgtg
     2761 aaaggctttg cgcagttccg ttgacggcgt gatttcttta tcccactcat caagggctaa
     2821 atcggttttt ttgataccgc gcggccagag gcggtcgacc agtatgcgat aaccatcgct
     2881 ctgttcagcc ggatcataaa cgcgtttgca ctgaatattc atcgtgtttg ttctcccttt
     2941 cacgcatgat ttgttacgtc actatagcac cggatattag cgaatttctt tgtctctccc
     3001 ggcgcaaagt ccgaaaatcg ccatcagtag tgaaagtatg gcaacaccca taagtggtac
     3061 agaccagtta ccgttagcat cgtgtatttt acccatcagc ggcggcccac aggctgccaa
     3121 caaatacccg acggattgtg ccatccccga gagtgccgcc gcctgatgcg cagaacttgc
     3181 ccgaagacca atgaacgtca accccagtat cattgttgcg ccggaaccaa aaccgaaaag
     3241 cagagtccag gtgatcgcgt gcgctggcat aaagcagagc ccaacagcgc ccactgcgca
     3301 catcaaggca acgaacgctg caataccacg ctgatctttc acatgatgta agaaaagagg
     3361 gatcagcaaa ccgggagctg ctgtggctag ttgcagcaaa ccatgcagtg aacccgcctg
     3421 tgcttcgcta tagccgtgac tgatgaggat cgccggaagc cagccaataa tcacgtaata
     3481 gaccagtgag ttgatcccaa gaaacaatgt gacctgccag gcaagcggcg aacgccagat
     3541 accccgagta tgtaaggcgc gcgaggtact caaatttgca tgttgttggc ttcgccactg
     3601 tggcagccat aaaaaaagag ccagcagagg aaaacacatc agcatgagca acgcgccttg
     3661 ccagccaaaa ccgttcaaag ccagcggcac gaccatagcc gatccaagtg ccgccgcagc
     3721 tcccattgtc agggaatatg cgccggtaag tctggcgacg gaatgaggga aatcgcgttt
     3781 aattaatcct ggcagtaaga cattgcctaa tgcaatccca ccgccaatga ccgctgtacc
     3841 gccaaataat aagtaaggcg aagggagaga gcgaattgcg ataccagcac agatcaaaag
     3901 taacgcggca aacaggctac gttccatacc aaaacgtcgc gctaccgggg cggccaaagg
     3961 tgagattagc gcaaaggcca ataatggcag ggtggtcaat aagccggttt gcgctgtcgt
     4021 cagcgagtaa gcggaacgaa tcgtatcgag taacggtgct gcgccggtga aggtgacgcg
     4081 taatgttgtg gcaatcatca gaatgccagc gataaggaca atcctgtttt tgccgcttaa
     4141 tgaagttgaa caggtcatat gcttctcatt gttacaaacg aggctgtacc ttaccgtttt
     4201 tattgttatg ttgaatcaag ctaaaatgac aaattatcgc taaattcgga caaaatgatg
     4261 cacaggttga atctcaatgg ctatgaaccc gaccgacacc atgaagccgc ggtcgcattt
     4321 tgtattcacg ccggaacgga tgagttaacc agtcctgtac atcagcaccg caaaggacag
     4381 ctgatactgg cgctacatgg tgcaattacc tgtacggtgg aaaatgcttt gtggatggtg
     4441 ccgccacaat atgcggtttg gataccgggc ggggtgaagc atagcaatca agtgacagct
     4501 aacgctgaac tctgttttct gtttatcgaa cccagcgcag tgacaatgcc gacaacatgt
     4561 tgcacattga aaatatcgcc cttatgccgg gaattgattt taaccctggc aaacaggacg
     4621 acgactcagc gtgctgagcc gatgacccgc agattaattc aggtcctgtt tgatgaactc
     4681 cctcagcagc cacaacaaca gctgcacctg cccgtttctt ctcatcctaa aatccgcacg
     4741 atggtggaga tgatggcgaa agggcccgtc gagtgggggt cattggggca atgggctggc
     4801 ttttttgcga tgagtgaacg caatcttgcc cgactgatag taaaagagac ggggttgagt
     4861 tttcgccagt ggcgtcagca gttacagctg attatggcgc ttcagggatt agtgaaaggc
     4921 gatactgtac agaaagttgc tcatacgctg gggtatgatt caacaacggc ctttattact
     4981 atgtttaaga aaggcctggg gcaaacgcca ggccgatata ttgcgggatt aactactgtt
     5041 tccccacaat cagcgaaacc agacccgcgg caataagcgg gccaaccggt acgccgcgaa
     5101 acagcgccac gcctaaaaca gtgcctacca gcagtccggc gaccagttgc ggctggctgc
     5161 ccattaacgt cacgccgcgc ccacccaacc aggagacaat aacgcctact gcaattgcca
     5221 ccagtgattt ccagttcaga aacgagtgaa tcagggttga gggcggtagc gtcccgctgg
     5281 cgatgggggc cataacgcca atcgtcagaa tgattatccc gatacttagc ccctgttttt
     5341 caatccacgg gaaaaaagtg cttaacggtg tgacgcggac aatgattaac accagaattg
     5401 aaacggcgac agtgttgtta tgactgataa agcccagcgc agctaacccg agcaggatca
     5461 gcaaagtgac atcaaacatc gtggtttcct tgccaaaaaa taataagcct gcttacttta
     5521 cgcttgctgc aggaaggaag tagaaaatct ttacgttttt tgcataaaaa atcaggcgaa
     5581 ctgtcatcaa actgccattt gtgcacgcta acaataacgt gaaaacagct atctcccggg
     5641 ggcatgctat gaacgatcaa atgttcgtcg agacactgat tatcacgtca tcgttttttg
     5701 ccattgctgt tgtgctggtt ttgtccgttc tgcttattga acggacaggc tagttacgca
     5761 gtgcggcgga aattgagcag ttctggttgc gcaatgcgca aataatccgt ggttttcaaa
     5821 ataacggatt tatccagcat ccctgcattg aaggcgattt catcaaatcg ttcgaagagt
     5881 agagggtcgg caaccagttt gagttttgga tggaagctga aaggggggat cgcgccgaag
     5941 acacagcctg tgagttcatc gacttcagca gggctggcta acgaagcgcg taatccgcct
     6001 atatggctgg caagttggct cagatcggcc tgttgatcgg cagcgaggat cgccagaaca
     6061 tgttgattta cgccattgcc tttgacttta cataccagtg ccttagctcc ctggcctaaa
     6121 gcggtgccac gaatttctga cacagcttca catttaccta ccgcttcatg ggtaacgacg
     6181 cggaagttag caccttcttg tgataataaa gcgattaacc gctgatgcgt cacgcttcct
     6241 ttagccattt cagtcatttc acgctcctga gattacaagc aaacaaccac agaaggttat
     6301 catgaacggc tgtttttgtt ctgcttattg acatataaac gctcatcgga agctttatac
     6361 gcatcatgta acgtatcgtt ttctttcatc gcgtaaatac cggaagagaa gccgatctct
     6421 ttctgcggcg cgatatgttg cagacgtttt tcgatacgtt caggcagttg tgctgcaatt
     6481 tgcggcgtcg aatcgacaag aatgatgcag aattcatcgc caccgagtcg gatggcataa
     6541 tcacttttac gaatcgattg tttaatcgcc tgagctaata acgtaatcgc taaatccccc
     6601 tcctgatgac cgagggtgtc atttatttgc tttaacttgt ccatgtcaat tgcaataaac
     6661 atcaccgaag aaccggcttg caccagtttc tgcaaccgct gctccagttc aggggttaaa
     6721 attttgcgat tatacagtcc agtcatcgca tcactaatat tttctcgcga cacattttgg
     6781 tataaacgga agtgcatccg caccatattc agcaaaatta acgccgtcaa aatccagaac
     6841 agaacgcttt tccacgaaga cgtgataaag taaagaatat caatggataa cgagacacga
     6901 atgccgcccg gtaagtcatg gacgtaactg acatactgga acagattatc ttcgctctgg
     6961 ttgatgataa tgtcgcgccc cgaatcggta tcggttagcg tgacattgag aaaacgccag
     7021 aggagagggc ggtcatgagt gtaaaagata ttccgcaaat tgtttttgtt aatatccagc
     7081 agcacaatcc cttttagctg ccctgcgaca tataccggcg tcagcaaact catcacgttc
     7141 tgtttagtac gctgatcctg gtaaatgctc gacaatacgg tacgcccggt aaataccttt
     7201 tcaatatctt tgcgatcgat gccaatactt cctgactgca ggaaggacca gttattcatc
     7261 gcaaagtatt caacattaac cggctcataa aaatagacat agcggtcatt gagatcgaga
     7321 taatagtgaa atttctcttc ggccatccca agacctgaac cgtaatcatc ctggtggcgg
     7381 ttgccttcca ctgcctgatc aaaagcgggt aatagtgctg cagcttccac aattgtgtcg
     7441 cagtcagtgg atgcggtttg tagtgtgccg cgtaagcccg ggtatttatg ggcagtcagg
     7501 tttaagccat gggtattatt ggcagactca aaagcctcgc agatggcgcg ccgctgttct
     7561 ggcgacactt ccgactgctc agcagcgaga gcgcgcatca catgcgcggc aatactctga
     7621 ttctgatact tatcataaag aaaggaggag tctgctttct gcacgatata gcgcagatag
     7681 gcactcattg ctctgtcgct ggcgacaagt tcaaaaataa ggaaggaaga tgtcagcaca
     7741 atgacacttg cggcgataaa atgccggagc attctatggt gcaatttcat gataaatggc
     7801 actccttcgg ttagtgaaac gagaaaacca gataaagttc gattgcggcc tggcacaggg
     7861 gcgctaatta tactcatctc tgcgccgctg gggaggggaa atttaggggt aatagtaata
     7921 aattgtatgc aaaagccacg caaaaagcat ggctctgaat atttttgcac ttaaatatga
     7981 tgaataatca ctttgttgcc cccctcgctt ttcgcttcgc gtaaggcttt atctgcttcc
     8041 gtcatgacaa gcgagatatt acgcgtgtca cccgttgaaa aaaccgtgcc aatactgatg
     8101 gtcatttgtt caggaacatc atatctgtta ctaaagccgg tcaggagttc gacattttta
     8161 cgcatacgtt ccgcaatgat ttttgcctgg gcactattga gttcggtaaa tagcaagcca
     8221 aacacctcac cctccagtcg cgctaaaata tcatcggggc ggatgctttg ctggataata
     8281 tcgacaattg aaaccagcac tttatcaccc acacgatgtc cccattgggc attgatgcgt
     8341 ttgaactgat taatatcaac cagcatgaca cagaagtcct taacaacagg tcgcgacaat
     8401 aatgactcta cgctgttgaa aaagtagcgc cgattataaa tattggtcag cacatcatga
     8461 actgccagtt tgctggagag ttgcagttcc tgaaaaatgt tataaatcag aaaagacaca
     8521 acaaacagtt tactggagac ttcaatcccg cgactgatgt accagatggt gtaattatac
     8581 tcatccagag tcagtagaag taagttgcag catagcgatg ccagacataa taatgctatt
     8641 aatggccata gttcgctggc taatcgtgta cgcatgataa taaagaacag taaaaatgcc
     8701 cacagacaaa ccaatatttt tgtatagttg attccccagg tcgcagtatg gttgtcagga
     8761 cagtagtcga cgacatacaa agaatagtca gcgttataac tgctcagatt gtgtgccaga
     8821 aggggaaaaa ctaaaaaagg aatcagcgcc agcaacagga ttcccgtttt tttcttattg
     8881 ttgtcaagga tgttgtcttt tccataacaa aagagtgcca gcgaggttaa acaaataaaa
     8941 ctgagttggc ggaaaatata gaaaatagaa acatcattcg actttgtctg gatcagtgta
     9001 ctgccgttaa ttggtttatg gataattaca atggtttcaa ctaaatagat aaggccactt
//
LOCUS       DAPHBJ010000174         8943 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.176, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000174 DAPHBJ010000000
VERSION     DAPHBJ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.176"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..520
                     /gene="fecI"
                     /locus_tag="RHZ76_003646"
     CDS             <1..520
                     /gene="fecI"
                     /locus_tag="RHZ76_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HDV2814220.1"
                     /translation="SDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQDT
                     FLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPEE
                     RESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKAV
                     EHCLLFRLEYGL"
     gene            517..1470
                     /gene="fecR"
                     /locus_tag="RHZ76_003647"
     CDS             517..1470
                     /gene="fecR"
                     /locus_tag="RHZ76_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HDV2814221.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1557..3881
                     /gene="fecA"
                     /locus_tag="RHZ76_003648"
     CDS             1557..3881
                     /gene="fecA"
                     /locus_tag="RHZ76_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HDV2814222.1"
                     /translation="MTPLRVFRKTTPLVNTIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGRGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYLNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIASYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            3926..4828
                     /gene="fecB"
                     /locus_tag="RHZ76_003649"
     CDS             3926..4828
                     /gene="fecB"
                     /locus_tag="RHZ76_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HDV2814223.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNKTYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            4825..5823
                     /gene="fecC"
                     /locus_tag="RHZ76_003650"
     CDS             4825..5823
                     /gene="fecC"
                     /locus_tag="RHZ76_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HDV2814224.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            5820..6776
                     /gene="fecD"
                     /locus_tag="RHZ76_003651"
     CDS             5820..6776
                     /gene="fecD"
                     /locus_tag="RHZ76_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HDV2814225.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            6777..7544
                     /gene="fecE"
                     /locus_tag="RHZ76_003652"
     CDS             6777..7544
                     /gene="fecE"
                     /locus_tag="RHZ76_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HDV2814226.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(8102..8359)
                     /locus_tag="RHZ76_003653"
     CDS             complement(8102..8359)
                     /locus_tag="RHZ76_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814227.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            8506..8763
                     /locus_tag="RHZ76_003654"
                     /pseudo
     CDS             8506..8763
                     /locus_tag="RHZ76_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8776..>8943)
                     /locus_tag="RHZ76_003655"
     CDS             complement(8776..>8943)
                     /locus_tag="RHZ76_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDV2814228.1"
                     /translation="TNGLIRQYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPK
                     EIIERGVALTD"
BASE COUNT         1968 a         2536 c         2491 g         1948 t
ORIGIN      
        1 gtctgaccgc gccactacca cagcttcctt aacgttcgag tcgctttatg gcacacatca
       61 cggctggttg aaaagctggc tgacgcgcaa actccagtct gcttttgatg cagatgacat
      121 tgcccaggac acttttttgc gggtaatggt cagcgaaacg ctctcgacga tccgcgatcc
      181 tcgctccttc ctctgcacta tcgccaaacg cgtgatggtg gacctgtttc gccgaaacgc
      241 gctggaaaaa gcgtatctgg agatgctggc gcttatgccg gaggggggag cgccttcacc
      301 tgaggaacgc gaaagccaac tcgagaccct acaactcctc gacagcatgc tggacgggct
      361 aaacggcaaa acacgtgaag cgtttctgct ttcgcaactg gatggtctga catacagcga
      421 gattgcgcac aaactcggtg tttccatcag ctccgtgaaa aaatacgtgg cgaaagccgt
      481 cgagcactgc ctgctgttcc gtctggagta tgggttatga atcctttgtt aaccgattcc
      541 cgccgtcagg cgctgcgttc agcttcccac tggtatgccg tgctaagcgg cgagcgcgtc
      601 agcccacaac aggaagcgcg ctggcaacag tggtatgaac aggatcagga taaccagtgg
      661 gcctggcagc aggttgaaaa cctgcgcaac cagcttggcg gtgtgcctgg cgacgttgcc
      721 agccgggcgt tgcacgatac ccgcctcacc cgccgtcacg tgatgaaagg attactgctg
      781 ttgctcggcg ctggcggagg ctggcagctc tggcagtcgg aaaccggcga aggtctgcgg
      841 gcagattacc gcaccgccaa aggcacagtc agccgtcagc aactggaaga tggctccctg
      901 ctcacgctga atacccaaag cgcggcggat gtgcgttttg atgcgcatca gcgcaccgtc
      961 cggctctggt acggtgaaat cgccattact accgcgaaag atgccctgca acgccccttc
     1021 cgtgtcctga cccgtcaggg ccagctcact gctttaggga cagaatttac cgtccgtcag
     1081 caggataatt tcacgcagct tgacgtgcag cagcacgctg tggaagtgct tctcgccagt
     1141 gcccccgcgc aaaaacgcat cgtgaacgca ggtgaaagcc tgcagttcag cgcctctgag
     1201 tttggcgcag tgaaaccgct ggatgacgag agtacaagct ggacgaagga catcctgagc
     1261 ttcagcgata aaccgctggg tgaggtgata gccacgctaa cccgttaccg caacggcgtg
     1321 ctgcgctgcg atcccgccgt tgccgggctg cgcctgagcg ggacgttccc gctgaaaaat
     1381 accgatgcga tcctgaacgt tatcgcgcaa acgcttcccg ttaaaattca gtctattacg
     1441 cggtactgga taaacatttc accactgtaa ggaaaataat tcttatttcg attgtccttt
     1501 ttacccttct cgttcgactc atagctgaac acaacaaaaa tgatgatggg gaaggtatga
     1561 cgccgttacg cgtttttcgt aaaacaacac ctttggttaa caccattcgc ctgagcctgc
     1621 tgccgctggc cggtctctcg ttttccgctt ttgctgcaca ggttaatatc gcaccgggat
     1681 cgctcgataa agcgctcaat cagtatgccg cacacagcgg atttaccctc tcggttgacg
     1741 ccagcctgac gcgcggcaag cagagcaacg gcctgcacgg cgattacgac gtcgagagcg
     1801 gcctgcaaca actgctggac ggcagaggac tgcaggtaaa accgctggga aataacagct
     1861 ggacgctgga gcccgcgccc gcaccaaaag aagatgccct gaccgtggtc ggcgactggc
     1921 tgggtgatgc gcgtgaaaac gacgtatttg aacatgctgg cgcgcgtgac gtgatccgcc
     1981 gtgaggattt cgccaaaacc ggcgcaacca ccatgcgtga ggtacttaac cgcatccctg
     2041 gcgtcagcgc gccggaaaac aacggcaccg gcagccacga cctggcgatg aactttggca
     2101 tccggggcct gaacccgcgc ctcgccagcc gctcgaccgt cctgatggac ggcatccccg
     2161 tccccttcgc cccttacggt cagccgcagc tttcactggc tcccgtttcg ctcggcaaca
     2221 tggatgccat tgacgtggta cgcggtggtg gtgcggtgcg ttacggaccg cagagcgtgg
     2281 gcggcgtggt gaactttgtt acccgtgcca ttccgcagga ctttggtatc gaggcgggcg
     2341 tggaaggtca gctcagccca acctcttcac aaaacaaccc gaaagagacg cacaacctga
     2401 tggtgggcgg cacagcggac aacggttttg gcaccgcgct gctctactcc ggcacgcgcg
     2461 gcagtgactg gcgcgagcac agcgccaccc gcatcgacga cctgatgctg aaaagcaaat
     2521 atgcgccgga tgaggtgcac accttcaaca gcctgctgca atattacgac ggtgaagccg
     2581 acatgcccgg tggcctgtct cgcgcggatt acgacgccga tcgctggcaa tccacccgcc
     2641 cgtatgaccg cttctggggt cgtcgcaagc tggcgagcct gggctaccag ttccagccag
     2701 acagccagca taaattcaac attcaggggt tctacaccca aaccctgcgc agcggctacc
     2761 tggagcaagg caaacgcatc accctctcgc cgcgtaacta ctgggtgcgc ggtattgagc
     2821 cacgctacag ccagatcttt atgatcggcc cttccgcgca cgaagtgggc gtgggctatc
     2881 gctatttgaa tgaatcaacg catgaaatgc gttactacac cgccaccagc agcgggcagt
     2941 tgccgtccgg ctcaagccct tacgaccgcg atacgcgttc cggcaccgag gcgcacgcct
     3001 ggtatctgga tgacaaaatc gacatcggca actggaccat cacgccgggt atgcgtttcg
     3061 aacatatcgc gtcataccag aacaacgcca tcacaggcac gcacgaagaa gtgagctata
     3121 acgcaccgct tccggcgttg aacgtgctct atcacctgac tgacagctgg aatctttatg
     3181 caaacactga aggctcgttc ggcaccgtac agtacagcca gattggcaag gctgtgcaaa
     3241 gcggcaatgt ggaaccggaa aaagcgcgaa cctgggaact cggtacccgc tacgacgacg
     3301 gcgcgctgac ggcggaaatg gggctgttcc tgattaactt taacaatcag tacgactcca
     3361 accagaccaa cgacaccgtc actgcacgtg gcaaaacgcg ccataccggg ctggaaacgc
     3421 aggcacgtta cgatctgggt acgctaacgc caacgcttga taacgtttcc atctacgcca
     3481 gctatgcgta tgtgaacgcg gaaatccgcg agaaaggcga cacctacggc aatctggtac
     3541 cattctcccc gaaacataaa ggcacgctgg gcgtggacta caagccagga aactggacgt
     3601 tcaatctgaa cagcgatttc cagtccagcc agtttgcgga taacgccaat acggtgaaag
     3661 agagcgccga cggcagtacc ggccgcattc ccggcttcat gctctggggc gcacgcgtgg
     3721 cgtatgactt tggcccgcag atggcagatc tgaacctggc attcggtgtg aaaaacatct
     3781 tcgaccagga ctacttcatc cgctcttatg acgacaacaa caaaggcatc tatgcaggcc
     3841 agccgcgcac gctgtatatg caggggtcgt tgaagttctg atgtgttaac gcccggcttg
     3901 ccgggctttt agctggaatg tgattatgtt ggcatttatc cgttttcttt ttgcaggcct
     3961 gctgctggtg atcagccacg cctttgccgc catggttcag gacgaacacg gcacgtttac
     4021 actcgaaaaa acgccacaac ggattgtggt gctggaactc tcgttcgccg atgcgctggc
     4081 cgccgtggac gtcagcccga tcggtattgc cgacgataac gatgcaaaac gcatcctgcc
     4141 cgaagtgcgt gcgcacctga aaccgtggca gtccgtcgga acgcgcgcgc agccgagcct
     4201 ggaagccatt gccgctctga aaccagacct gatcattgcc gacagcagtc gccatgcggg
     4261 ggtttacatc gccttgcagc aaatcgcgcc ggtactgctg cttaagtccc gcaacaaaac
     4321 ctacgctgaa aatttgcaat ctgcggctat catcggcgaa atggtgggta aaaagcgaga
     4381 gatgcaggca cgtctggaac aacataaaga gaggatggcg cagtgggcca gccagcttcc
     4441 caaagggaca cgcgtggcct ttggcacatc acgcgaacag caattcaacc tgcatactca
     4501 ggagacctgg accggcagcg tgctggcctc tctggggctg aacgttcccg ctgcgatggc
     4561 gggtgcgtcc atgccgtcca tcggcctgga gcaactgctg gcggtcaatc ctgcctggct
     4621 gctggttgcc cactatcgcg aagagagcat tgttaaacgc tggcaacaag atccgctctg
     4681 gcagatgtta accgccgcgc agaagcagca ggttgcttcg gtcgacagta acacctgggc
     4741 gcggatgcgc ggtatttttg ctgcagagcg tattgccgct gacacggtaa aaatcttcca
     4801 ccatcagccg cttaccgttg tgaaatgacc gcgataaaac acccggtgct gctgtggggg
     4861 cttcccgttg cagcacttat tattattttc tggctgagtc tgttttgcta ctcggccatt
     4921 cctgtttccg gagcagatgc aacccgcgcc ctgctgcctg gacacacgcc aacgctacca
     4981 gaagcgctgg tgcaaaacct tcgtttgcca cgaagcctgg tcgccgttct gatcggcgca
     5041 agcctggcgc tcgcgggcac gctgctgcaa accctgaccc acaacccaat ggcctctcct
     5101 tcactgctcg gcattaacag cggcgcggcg ctggctatgg cgcttaccag cgcgctgagt
     5161 ccgacgccga ttgcaggcta ttctctgtcg ttcatcgcgg catgcggggg cggcgtgagc
     5221 tggctgctgg tcatgaccgc aggaggcgga tttcgtcata cccatgacag aaacaaactg
     5281 atcctcgcgg gtatcgcgct gtcggccttt tgtatgggcc ttacccgcat caccctgctg
     5341 ctggccgaag atcatgctta cggcatcttt tactggctgg caggcggagt gtcccacgcc
     5401 cgctggcagg atgtctggca gctcttgccg gtggtggtca ctgcagtccc tgtcgtgtta
     5461 ctgctggcga atcaactgaa cctgctcaac ctcagcgaca gcaccgccca tacgctggga
     5521 gtgaacctga cgaggctacg tttggtcatc aatatgttag tgctgcttct ggttggtgcg
     5581 tgcgtcagcg tggcaggtcc ggtggcgttt atcggtctgc tggtgccaca tctggcgcgc
     5641 ttctgggcag gcttcgatca gcgcaacgta ctgccagtga gcatgctgct gggggccacg
     5701 ctgatgctgc tggcagatgt actcgcacgc gcgctggcct tccccggaga tctgcccgca
     5761 ggcgcagtgc tggcgctgat tggcagccct tgctttgtct ggcttgtgag gaggcgagga
     5821 tgaaaattgc gctggttatt ttcatcaccc ttgccctggc gggctgtgcg ctgttatcac
     5881 tccatatggg agtgatcccc gtgccgtggc gcgcgctgct gaccgactgg caggccggac
     5941 gcgagcatta ttatgtattg atggagtacc gactgccgcg cttgctgctg gcactgtttg
     6001 tcggtgcagc cctcgccgtg gcgggcgtgc tgatacaggg gattgtgcgc aaccctctgg
     6061 catcaccgga tattctcggc gttaaccatg ccgccagcct ggcctctgtg ggggctctac
     6121 ttcttatgcc gtcactgccc gtgatggtgc tgccgctgct ggcctttgcg ggcggcatgg
     6181 cggggttgat attactgaag atgctggcaa agacccacca gccgatgaag ctggcgctca
     6241 ccggcgtggc gctttctgca tgctgggcca gcctgacgga ttatctgatg ctctcgcgcc
     6301 cgcaggatgt gaacaacgcc ctgctgtggc tgaccggcag cttatggggc cgtgactgga
     6361 gctttgtgaa gattgccatc ccgctgatga ttttatttct gccgctgagc ctgagttttt
     6421 gccgcgatct cgacctcctt gcactcggcg atgcgcgcgc caccacgctc ggtgtgtcgg
     6481 tgccccatac ccgattctgg gctttgttac tagctgtcgc catgacatct accggcgtgg
     6541 ccgcctgcgg cccgattagc tttattggtc tcgtggtgcc gcatatgatg cgtagcatca
     6601 ccggtggacg tcaccgcaga ctgctgcctg tttcggccct gacaggtgcg ttgctgttgg
     6661 tggttgccga tctgctggcg agaattattc atcccccact ggagctcccg gttggcgtgc
     6721 tgaccgccat tatcggtgcg ccgtggtttg tctggttgct tgtgagaatg cgataaatga
     6781 ctttacgaac tgaaaatctg acggtcagtt acgggacaga caaggtactt aacgacgttt
     6841 cactctcact gccaacgggg aagatcaccg ccctgatcgg tcctaacggt tgcgggaaat
     6901 cgacgctgtt aaactgtttt tcgcggcttt taatgccgca gtctggcacc gtatttctcg
     6961 gcgataatcc cataaatatg ctctcatcac gccagttggc ccgcaggctt tcgctgctgc
     7021 ctcagcacca tttaacgcca gaggggatca cagtccagga gctggtttcg tatggtcgta
     7081 atccctggct gtcactctgg gggcgtctct ccgctgaaga caatgcacga gttaatgtcg
     7141 ccatgaacca gacccggatc aatcatcttg ccgttcgtcg gttaaccgag ctttccggcg
     7201 gtcagcgcca gcgcgcattt ctggcgatgg tcctggccca gaatacgccc gttgtattac
     7261 ttgatgagcc aaccacctat cttgatatca atcaccaggt ggacctgatg cggttgatgg
     7321 gcgaactccg gactcagggg aaaacggtgg tcgctgtgct gcacgacctt aatcaggcta
     7381 gccggtactg cgatcaactg gtggtaatgg caaacggaca tgttatggcg caaggcacac
     7441 cagaagaggt gatgacccca ggattgctga gaacagtatt cagcgtggaa gcggaaatac
     7501 accccgagcc ggtatctggc aggccgatgt gcctaatgag gtagattgca caggccgtaa
     7561 gaaccaaacc acgactgaat gaaactggac tggcgccagc aagcctgttc agactggggc
     7621 tgaacttttc cggactctga aagattacca atactcatcg tccatccgct tgctttaggc
     7681 tgacaggttc ataatcaacg caaaccagag ctgtacaggc ttgggcgcgg ctttcaaacc
     7741 agtcgtgatc acagcaatca attttgaact ctgcttaacg gacatttctg tataaccctt
     7801 acggcaacga aaaacgcgaa gttaaaattt tagaaaccca aaaacgtgac atgactaagt
     7861 ttagatttca gggggggaga tcaaaaaatt tcgctctgtg ccagagcgga cattcacgga
     7921 gctggttcat taccaatgag gttgggcttt tgaggataaa tcaatgatca gacgccaacg
     7981 taaatcaaaa gcaaccctgg aacggaattg ctaatccagt ttctgaccat cgatttttct
     8041 aaaaagtgtg cgtttgctac tacttaggta ggtgcagctt tcttaatcac cggcagccac
     8101 gttatacagg ccagttgatg gatcgattgt tatcaatgat atcttcatga gtcggtgtct
     8161 cacccagctt accgaagctg gcataaagtg aaggcagacg ggcccgtcct tctccctttt
     8221 tcgccagagg gaaagcgcga agcatggtgg cagcctccca gtcacaacaa taggatggtg
     8281 tgcacggctg ctgacgccat gattcagcga tagagccgga aaatacgggg tcaaagccgg
     8341 tatcattaac cagagtcatc gtgatctgta ctgcggctgg atgatctcct gctactgcaa
     8401 ctgccaggcg accgcggctc ccttcaagta gtctgaagtg gtttactgaa tttggccacc
     8461 tgaacagagg tgatatgctc acctcagaac aacacaggtg ccataatgaa aaaaagaaat
     8521 ttcagcgcag agtttaaacg cgaatccgct caactggtcg ttgaccagaa ttacaccgtg
     8581 gcagatgcag ccagcgctat ggatgtcggc ctttccacaa tgacgcgatg ggtgaaacaa
     8641 ttacgtgatg agcggcaggg aaaaacacca aaagcctccc ccattacccc ggaacaaatt
     8701 gaaatccgtg agctcaggaa aaagctacaa cgtattgaaa tggaaaatga aatattaaaa
     8761 aaggctactg tagattcaat ctgtcaatgc aacacccctt tcaattatct ctttcggtgt
     8821 tttgaacttc agtgtctttc tcggtctgtt gtttagctga gcagcaacca gatctagttc
     8881 atgttgagta tattgggcaa gacatgtctt tttaggaaag tactgccgaa ttagcccatt
     8941 tgt
//
LOCUS       DAPHBJ010000175         8840 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.177, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000175 DAPHBJ010000000
VERSION     DAPHBJ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.177"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..813
                     /gene="gadX"
                     /locus_tag="RHZ76_003656"
     CDS             <1..813
                     /gene="gadX"
                     /locus_tag="RHZ76_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HDV2814229.1"
                     /translation="HGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCV
                     ENFVFVSRDTLSLFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQNKNL
                     RYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARI
                     ASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVS
                     YFIYVFRNYYGMTPTEYQERSAQRLSNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1181..1909
                     /gene="gadW"
                     /locus_tag="RHZ76_003657"
     CDS             1181..1909
                     /gene="gadW"
                     /locus_tag="RHZ76_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HDV2814230.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2272..5385)
                     /gene="mdtF"
                     /locus_tag="RHZ76_003658"
     CDS             complement(2272..5385)
                     /gene="mdtF"
                     /locus_tag="RHZ76_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HDV2814231.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLAAGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5410..6567)
                     /gene="mdtE"
                     /locus_tag="RHZ76_003659"
     CDS             complement(5410..6567)
                     /gene="mdtE"
                     /locus_tag="RHZ76_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HDV2814232.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            6627..6905
                     /locus_tag="RHZ76_003660"
     CDS             6627..6905
                     /locus_tag="RHZ76_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814233.1"
                     /translation="MRDLQTSGIVGLSTSKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(6906..7433)
                     /gene="gadE"
                     /locus_tag="RHZ76_003661"
     CDS             complement(6906..7433)
                     /gene="gadE"
                     /locus_tag="RHZ76_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HDV2814234.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDSERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8232..8804)
                     /gene="hdeD"
                     /locus_tag="RHZ76_003662"
     CDS             complement(8232..8804)
                     /gene="hdeD"
                     /locus_tag="RHZ76_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HDV2814235.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT         2365 a         2062 c         2067 g         2346 t
ORIGIN      
        1 catgggaatt gtctaattgc gtatgcaaga cataaatata ttctcaccat ggttaatggt
       61 gaatatcgct attttaatgg cggtgacctg gtttttgcgg atgcaagcca aattcgagta
      121 gataagtgtg ttgaaaattt tgtattcgtg tcaagggaca cgctttcatt atttctcccg
      181 atgctcaagg aggaggcatt aaatcttcat gcacataaaa aagtttcttc attactcgtt
      241 catcactgta gtagagatat tcctgttttt caggaagttg cgcaactatc gcagaataag
      301 aatcttcgct atgcagaaat gctacgtaaa agagcattaa tctttgcgtt gttatctgtt
      361 tttcttgagg atgagcactt tataccgctg cttctgaacg ttttacaacc gaacatgcga
      421 acacgagttt gtacggttat caataataat atcgcccatg agtggacact agcccgaatc
      481 gccagcgagc tgttgatgag tccaagtctg ttaaagaaaa aattgcgcga agaagagaca
      541 tcatattcac agttgcttac tgagtgtaga atgcaacgtg ctttgcaact tattgttata
      601 catggttttt caattaagcg agttgcagta tcctgtggat atcacagcgt gtcgtatttc
      661 atttacgtct ttcgaaatta ttatgggatg acgcccacag agtatcagga gcgatcggcg
      721 cagagattgt cgaaccgtga ctcggcggca agtattgttg cgcaagggaa tttttacggc
      781 actgaccgtt ctgcggaagg aataagatta tagagtttta ctcagacata aaaaaaaccc
      841 ggcatagggg accgggaaga ggatagtctg ccgtctccag actaataaac cgttataaca
      901 ctccctgttg gcacgggaaa ctttgtgctc tcagtaagtt aaatataact tttactggaa
      961 ataagatcag ccattttttt ataaacataa gctatacgct gtgcgaaaat atattcgtgc
     1021 tgcatttact tattatcaat taactgttat gcaaaactac tttgtggata aattttggtc
     1081 ctaccaaatc tggcagtttt tgcgctaaga aacagtctgg catcatttca ttagtatact
     1141 gaaattgaaa taatcgcagt atgaaatata agggataatc atgactcatg tctgctcggt
     1201 gatcctcatt cgtcgttcat tcgatattta tcatgaacag caaaaaatat cgctgcataa
     1261 cgagagtatt ctgctgctgg agaaaaattt ggcagacgat tttgcgtttt gttcaccgga
     1321 tacgcgacga ctggatatcg atgagctgac agtttgccat tacttacaaa atattcgtca
     1381 gctaccacgc aatttagggt tacacagcaa agaccgtttg ttaattaacc agtcaccccc
     1441 catgccgctg gtgacggcga tttttgatag cttcaatgaa tccggggtaa attcaccgat
     1501 actgagcaat atgctctacc tttcctgttt atcgatgttt tctcataaga aagaactgat
     1561 ccccttactt ttcaatagca tcagcactgt ttcaggaaaa gttgaacgcc ttattagctt
     1621 tgatatcgcc aaacgttggt atctgcgcga tatcgcggaa agaatgtata ccagcgagag
     1681 tctaatcaaa aaaaagttgc aggatgaaaa tacctgtttc agtaaaatat tactcgcctc
     1741 caggatgtcg atggccagac gattactcga gttacgtcaa attcctctgc atactattgc
     1801 ggaaaaatgt ggctatagca gtacatcgta ctttataaac acatttcgac aatattatgg
     1861 tgtaacgcca catcagtttg cgcaacattc gccaggtacc ttttcctgac atatttcgca
     1921 tttgaatatt ggtcaggatc tcacacctgc ttcatgtgaa actcttccct gatgatttct
     1981 gccgggctac cggctagttc tctttcgcag tttgccaggc ggtatcggta ttttcaggag
     2041 gaaaagactc ggcatgtttg ggattattaa gctgacaatt catacgatta cgggaatgtg
     2101 ggtcagtatt gtgttgttca aattgatgac gaatggctgg agtggtttct actttcagtg
     2161 ctgtgtacta agcctggtat tcctgacggt tagctggttg ttgagtggtg aatggttagc
     2221 aggaaagagt aaggctgaac cttcacgttc aaccttactc tcatttacac gttacgcttt
     2281 tttaaagcgg gcaaagagat gttccactac aacgaaaaag accggaacga agtaaattgc
     2341 cagcactgtt gcggcaaaca tcccgcccat cacgccggta cctaccgcgt tttgcgcgcc
     2401 agaaccggca ccatgactga taaccagcgg cagcacgccg agaataaagg ccagagaggt
     2461 catcaggatt gggcgtaaac gcatccgcgc cgcttcgatg attgcctcta tcggcgtttt
     2521 cccttctttc tgcatcatct caacggcaaa ttcgacaatc aggatggcgt ttttggcgga
     2581 aagcccgatg gtggtcagca aaccaacctg gaagtagacg tcattactta agccgcgcag
     2641 atcggtggcc agtaatgcgc caacgacgcc taacggaaca accaacatca ccgagaacgg
     2701 aattgaccag ctctcataga gtgcggcgag ggcgaggaac accacgacca gtgaaatcgc
     2761 atacagcgca ggagcctgat ttgaggataa cgcttcctga tacgatagtc cggtccatga
     2821 gtagccgacg cctgccggaa gtttagcgac caggtctgcc ataaatttca tggcgtcacc
     2881 ggtacttttc ccggccgccg cttcacctaa aatctccatt gacgggatgc cgttgtagcg
     2941 ttccagtcgc ggtgaaccat aggtccattc ggtagacgag taggcagaaa gcggtgccat
     3001 cgtgccagag gcgttgcgta cataccattg gttgatgtta tccggcaaca tacggaacgg
     3061 cgtgcctgcc tggacataca cttttttcac ccgcccctgg ttgaggaagt cgttcacgta
     3121 gctgctgccg aaggcggtgg aaattgtctg gttgatatca gacagcgcca cgcccatcgc
     3181 ttcagctttc gcagcgttga cgttcacttt gaacatcggc gtatcttcca ggccgttcgg
     3241 gcgtaccccg gtgacctgat tcggtgattg cgctgccagg gataacagct cgtttcgcgc
     3301 ctgggttagt ttttcgtgcc ccaggttacc gttgtccagc agttccatat caaaacctga
     3361 cgcggtaccc agttcagcca ccgcgggtaa gttgaacggg aagacgacgg ctttattgat
     3421 actgcttaac gcaatcattg cccgctgaat gatcgcggta accgagtttt cctcaccgac
     3481 acgttcagac cacggcttga gactgataaa cgccaggccg ttgttttgcc cctgaccgct
     3541 gaagccaaag ccgccaacgg taaacaccga ctggacatta tctttctctt tagtcagata
     3601 ataatccgtc acctgttgca gcactttcgt ggtgttaacc atggtggcac cggaaggtaa
     3661 ctgcgcggtg gtcataaata ccccctgatc ctcttctggt aagaaagagg tcggcgtgcg
     3721 caggaacagc accgccatcc cggcgcaaat cagcaggtag accaccatgt agcgaccggt
     3781 acaacgcaac agcgagcggg tgctatcggt atagtgttga gttgattttt caaacagcgt
     3841 gttgaagcgt gcgaacaggg cgttaggttt gtgaccgcct tccggcgcgg ctttcagaat
     3901 ggtggcgcac agggcagggg tcaggctcat tgccacaaat actgaaagca gcatggagga
     3961 gatcagcgtg atggagaact ggcggtagat ctccccggtt gcaccgctca taaaggccat
     4021 cggcataaac actgcggaaa gaacaacggc aataccgacc agcgcacgtt ggatctgccc
     4081 catcgattta tgcgtcgctt ccttcggcgg tagcttatct tccgcaatga cacgctcgac
     4141 gttctccacc accacgatgg cgtcatccac cagtaaccct atcgccagca ccatcccgaa
     4201 catagtcaac gtgttgatgg tgaaaccgac cgccgacaag atcgcaaacg tcccgagaat
     4261 aaccaccggt acggcaatcg tcgggatgat tgtggcacgg aaattctgca aaaacagata
     4321 catgaccagg aagactagga tgatagcctc aaccagtgtt ttgaaaactt cctgaataga
     4381 aatttcgata aacggcgtgg tgtcgtaagg ataaaccgtc ttcagacttg ccgggaaata
     4441 ggctgataag cggttcagtt cctctttgac tgcccgcgag gtatccaggg cgtttgctcc
     4501 ggcagccagt ttgatggcga tcccggcagc aggtttgccg ttatagcgcg ccacggtgga
     4561 ataatcttcc gccccaagtt cgacgcgagc gacatcacgc agcagcactt gcgaaccatc
     4621 ttgctgaact ttcaacagga ttttgccaaa ttcttccggc gtttgcagac gcgtctgcac
     4681 aatgatcgag gcgtttagct gctggtctgc cgcctgtggc atgccaccca gttgaccacc
     4741 ggaaatctgg ttgttttgca ccttaatctg ggaaataaca tcggaaggta ccaggttata
     4801 tttattgagt ttttgcgggt ccagccagat acgcatggca tactcggaac caaagagttg
     4861 tacgctacca acgcccgcgg tacggcttag cgggtctttg atattagacg ctacatagtc
     4921 cgcgatatcg tactggttga ggctgccgtt atcagaaata aacgccgcta ccatcaggat
     4981 attactgctc gacttatcga cgctaatccc ctgctgctgc actgcttcag gtaatgaagg
     5041 catagcgagt tgcagtttat tttgcacttg aacctgtgcg atatcaggag atgtcccagt
     5101 ctcgaaggtc agagtgatag aggcattgcc cgccgcatca ctggttgaag acatgtacat
     5161 caggccatca agcccattca tattttgctc aatcacctga gtgaccgagt cttctaccgt
     5221 ttgcgcatcg gcacctggat aggtagcgct gacggtaatg gtcggtggcg caatctgcgg
     5281 atactgcgca accggtaagt tcatgatcgc cagaccacct gcaagcatca taataatggc
     5341 aagtacccag gcaaaaaccg ggcgatcaat aaaatagtta gccatgaaag tccccttaag
     5401 cctgcaacgt tattgtttcg attcggtgct ggcgttttcc tggctggagg aaattgctcg
     5461 tgctttgata cccggacgaa tgcgttgcaa accggaaacg atcacccgat cgccagcctg
     5521 caagccagag gtgacgaccc actggtcgcc gatggctttg ctggcttcaa tttcgcgtag
     5581 ctgcacgaca tcgtctttat ccagaatgag cgccgttgct ttaccctggg cgttgtgggt
     5641 gacgccttcc tgcggcacca gtaatacatt ctggcggcta ccttcatcca ctaatgccgt
     5701 gacgtacatg ccaggcagca agtcaccatt tgggttgggg aaaatcgccc gtaacgtcac
     5761 ggagcccgtg gtttcatcca ctgtcgggtc ggagaatttc agcgtgccgg tctggctgta
     5821 gcgtttacca ttttccagat tgagctgtac tggcgtactg ccctgaacct gtttgatttg
     5881 cccactggcg acctcttctt tcatgcgtaa gaaatcttgc accgactgcg tgagatcgac
     5941 ataaatcggg tccagacgtt gtacggtaac cagcgaatct gcctgattag cggtaacgag
     6001 tgcgccgacg gtcaccgacg atttcccgct gacgcccgta atcggcgagg tgacattcgc
     6061 gtattgcaga ttgatcgtcg cctgttcaac agccgctttg gcgacggtga cattggcttc
     6121 tgcttcattc aactgggtgc gcgcggtgtc gtaatcctga cggctaacgt agttggtctt
     6181 cagcaacgat gcctggcggt taaaggtgat gcgggcattg ctggcggtag agagcgcttt
     6241 cgccagcgag cctttggcgg agtttagctc ggcctgtaaa ggtgcaggat caatctgata
     6301 cagcgaatcg ccctggttca ctttatcgcc ttcgataaag ttgcgtttaa tgataatacc
     6361 gcccacctgg ggacgtatct cggcaacttc ataaggaacg gttctaccgg gcaattcgct
     6421 caacacattg accgaaccgg gggagagtgt gacgacaccg acctcaggcg tcatggcggc
     6481 ggcgttttcc gccgatttgt catcgcaggc ggtgagcatc gcgccgcaga ataacaacgg
     6541 tattaacagc tttcttcttc tgttcatttt agtccctgaa aattcttgag aaataacggt
     6601 tctttaaatt gcgcgcgagg agagatatga gggatttaca gaccagcggc atagtggggt
     6661 tgtcaaccag taaggtggga tacaggcaca gtgatcgaca tggtgaggtc aacgacaaaa
     6721 aaaatgttgc cgttctgcca acagttcctg ccagcattcg ggcaacgaag ggttctactg
     6781 gtggatacac ataccagggg aataaagtcg ttttagcctc tcctttagta atcaccgggg
     6841 gtaatgaaat cagcatctgc atccctcgtc atgccagcca tcaatttcag ttgcttatgt
     6901 cctgactaaa aataagatgt gatacccagg gtgacgatgt cgctcatacg tcttagtttc
     6961 agcttgtaca taatgttgaa tttctgaatc tttaatgttt gttgcgaaat attgagcgtc
     7021 gacgtgatat tgctttcatt tttatttttc agaatcagtt gcataatttt gaactgatta
     7081 tgtgaaaaac ataattgtga attcttatgg ggcaagtgtt taccataagt aatctcaaga
     7141 agatttttga tactttcttt gcggctaaca aaataaatag gcgctttagc ttttagttta
     7201 ctgatgtgat aaggtgccaa aacaataatt ctctcggaat ctaatttctc cagaaattta
     7261 atcgcttctt catcaaggat atgattgtga taggtatcaa tgataacctt gaagggttta
     7321 ttgcctactt ttttgatctc tgacagggaa ttgattttta tcatttcgtg attatctttc
     7381 aactgccaaa agccctgtaa aagaaaagaa tctttcgtca tgagaaaaat cataacttgc
     7441 tccttagccg ttatcgtttc gaatatgtca tccttttcat tatttacatc cttgtccgaa
     7501 tcgttgttca atatagtaaa cgcctataag aactttaccc ttcgtgaact aatacgtcta
     7561 ttaacactaa tcaatacttc agatttatcc atcacgcact ccatatattg atcctaagta
     7621 agattcctgg ttgttatcag cttgtaaata gcaaacaaaa agttaacgtt tgcggattac
     7681 tgcccaagaa taagttatca ataaccagtt tgtgatctct gaagaatatt actaaagtta
     7741 aaatgtaatc cgatttaaat atcgagtctc cttgtttcga cttaagctgg caattggatt
     7801 gccagctttc ttttaacgca ttgaaattac ctgagtaaat aacgcattca tgtcttcagg
     7861 tttttatata gcgtctggag acacggatat ttatgcaatg taatcaacgc cagttgcaaa
     7921 aaaatgaacc cggattactt atttaaccgc ccatagcatc aacgcttacg gaactctttg
     7981 ttcactttcc cacagcactt ttaaaacatt aaacctgact acggaaaata tcagccatga
     8041 taatgtttga aatggctaat ttgccatgga gtgaaaaaaa ttagatgaaa ttcagtaggt
     8101 tgaaataatc actagcaggt tattatttca atgatagtgc gcaattgatc tacaacactg
     8161 cgtagcggag agagtattaa tcggatcata gtcacataaa gtgactatga tccgggtgac
     8221 aaccggggta attattgctg cttaacgaac aaactggcga agctgaacag gctggcggcg
     8281 ctaaatatca gttcaattcc caccagtgtg gaaaccagcg ttacagacac catcggcgtt
     8341 gcaccaagga atatccaggc aatgacgata tccagcacac caataacgag ctgtagccag
     8401 ctgcctttca ttgaacgctg acgataccaa ctcatcaggc gaataacccc tgcaacacag
     8461 aacaaaccgg caataaatgc cgcaatggca aaaatgccca gctccggtgc gcggatgaag
     8521 aaatagccga tcaataaata ggcgactgcg acgaggaaac cggataatac cggccagaaa
     8581 ttatgactgc ggttgctgaa taacccgaca ataagcgcaa tacccgagca gattaataat
     8641 gcacccacta ctgtgcttaa aatatcgcca gagacgaacg ggaaactgat acacagcaac
     8701 ccgacgataa acagcagcac ggcaataaac tggattgctc tgcgatgttt tttaagcatc
     8761 tccagatcaa acttcaaaat tgttgcctta tctatatata acatagaacc accctataaa
     8821 attaagaaga aaatcccctg
//
LOCUS       DAPHBJ010000176         8797 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.178, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000176 DAPHBJ010000000
VERSION     DAPHBJ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.178"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1143
                     /locus_tag="RHZ76_003663"
     CDS             <1..1143
                     /locus_tag="RHZ76_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDV2814236.1"
                     /translation="FIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVARN
                     MAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALITSE
                     IFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLNDL
                     IYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSTIRETVFSEANLENLSW
                     AAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSFIP
                     GIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTTPG
                     PLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            1171..1482
                     /locus_tag="RHZ76_003664"
     CDS             1171..1482
                     /locus_tag="RHZ76_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDV2814237.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILVHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            1538..3210
                     /locus_tag="RHZ76_003665"
                     /pseudo
     CDS             1538..3210
                     /locus_tag="RHZ76_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
     gene            3235..4674
                     /locus_tag="RHZ76_003666"
     CDS             3235..4674
                     /locus_tag="RHZ76_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDV2814238.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILARGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSERGWQIDPAGLRYSLNWLWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(4731..4949)
                     /gene="marB"
                     /locus_tag="RHZ76_003667"
                     /pseudo
     CDS             complement(4731..4949)
                     /gene="marB"
                     /locus_tag="RHZ76_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
     gene            complement(4981..5364)
                     /gene="marA"
                     /locus_tag="RHZ76_003668"
     CDS             complement(4981..5364)
                     /gene="marA"
                     /locus_tag="RHZ76_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HDV2814239.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(5384..5818)
                     /gene="marR"
                     /locus_tag="RHZ76_003669"
     CDS             complement(5384..5818)
                     /gene="marR"
                     /locus_tag="RHZ76_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HDV2814240.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            6029..6694
                     /gene="marC"
                     /locus_tag="RHZ76_003670"
     CDS             6029..6694
                     /gene="marC"
                     /locus_tag="RHZ76_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HDV2814241.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSAHIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(6719..7909)
                     /gene="ydeA"
                     /locus_tag="RHZ76_003671"
     CDS             complement(6719..7909)
                     /gene="ydeA"
                     /locus_tag="RHZ76_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HDV2814242.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8059..8797)
                     /gene="yneK"
                     /locus_tag="RHZ76_003672"
                     /pseudo
     CDS             complement(8059..8797)
                     /gene="yneK"
                     /locus_tag="RHZ76_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="putative protein YneK"
BASE COUNT         2408 a         1897 c         2115 g         2377 t
ORIGIN      
        1 tttattgccg gattactgca tctgaatagc attttcccca accttgaaaa agcacaagct
       61 atttttactc cggtaatgaa tggttctgta aatatcatgt caattatgat tgctttcctg
      121 gtcgccagga atatggcgat tagctatgag caagatgatc ttttatgcgg attaacggca
      181 ataggagcat tttttattgt ttatacgcca tatcagatga tagatgggca agcattcctg
      241 acgaccaaat atctcggcgc gcaggggttg tttgttgctg ttatcgttgc attgatcacc
      301 agtgaaatat tttgtcgctt agctcgaaac ccaaaaatca ccataacgat gccggcagct
      361 gtaccgcctg cggtagcgcg ttcatttaaa gttttattgc caatattttt tgtcatggtg
      421 ttcttttccg cacttaatta ttgcctgaca ctgatatccc cggcaggact aaacgatctt
      481 atttacacat taatccagac gccgctcaaa catatgggaa cgaatatctt tgcggtaatt
      541 atcctggggg ctgtgggtaa tttcctgtgg gtgctgggga tccacggacc taataccaca
      601 tcgacaattc gagaaaccgt tttttctgag gctaatctgg agaatctctc ctgggccgct
      661 caacatggca ctacctgggg cgcgccatat ccgattacct ggacttctat taatgatgca
      721 ttcgccaact gcggcggttc aggtatgacg ttggggttat tgttggctat ttttatcgct
      781 tctaagcgtg cggaataccg tgatctggca aaaatgtcat ttatccccgg tattttcaat
      841 atcaatgaac cgataatgtt tggccttcct attgtactta accccatcat gatggtgccg
      901 tttattatgg ttcccattgt taactgtgcc attggttact tctttgtttc gatggaaatt
      961 attccaccgg ttgcttatgc cgtgccctgg actacgcccg gacctttaat tgctttcctc
     1021 ggaaccgggg ggaactggct ggctttactg gttggttttt tatgtttagg tgtggcgaca
     1081 atgatctatt taccttttgt tattgccgcc aacaaagtca ataacatgac aactaacgga
     1141 taattccatt tacccaggaa ggagattgtg atgtttgcag atgaagaact tgttatggag
     1201 ttactggtca acgcggggca ggcacgttct gacgcgatgg aagccatacg ttgtgccggg
     1261 caaaaagact ggcagggcgc aactcagcta atggccagtt ctgaatctgc ctgtcttcag
     1321 gcacataaga ttcagaccgc attaattagt caggatgaag gctgtggcaa aataaaagtt
     1381 aatttaattc tggttcatgc acaggatcat ttgatgaatg caatattatg ccaggattta
     1441 gccagggaaa ttatttcatt aagaaaagaa ctccatgcct gattttttcc tccagtgcga
     1501 tagctggagg aaattattat actcttaagg ataagtcatg aatataaaga cgcttaacat
     1561 cagccttttg tctttttcta ttattacagc attgtttcca ttgaacgcga tggcaacaaa
     1621 attaaccata gagcagcgcc ttgaattgct tgaaaatgaa ttgtcgcaaa ataaacaaga
     1681 gctgaaagca acacagaatg aactgggagt atataaatcc cgactttcga ccttacaaaa
     1741 aagcatcacg gaaaacaaat ataaatcagc ctcgcttgct gaaatatcag cgacatctcc
     1801 cgtcgctgat aacatcaaaa atgaaaacgg tgaacagaac tcgtctgccg cagcgcatac
     1861 tataaatgga tcgcagcaag ttgccgttat tgaaagtaaa ggcgacaaaa ccactatcga
     1921 aagcgtgacc ctgaaagata tcagtaaata tataaaggat gatattgggt tcagctatca
     1981 ggggtacttc cgctcaggct ggggtactgg taatcacggc tcaccacaaa cttatgcagc
     2041 gggttctctg ggacgttttg gtaatgagat gagcggttgg tttgacctga ccttaaatca
     2101 gcgtgtttat aatcaggaag gtaaaacggc aaatgcggtc gttacctatg atggcaacgt
     2161 gggtgagcag tataacgatg cctggtttgg tgacagtgcc aatgaaaata tcatgcagtt
     2221 cagcgatatt tacctgacaa cgcgaggttt tttacccttc gcaccagagg cagacttctg
     2281 ggtcggcaaa cataaactcc cgcaatatga gatccagatg ctggactgga aaaccttaac
     2341 cacggatgtc gccgcgggtg tggggattga aaactgggca cttggtgtag ggctgtttga
     2401 tatgtcctta agccgtgatg atgtcgatgt ttactctcgt gatttttcgc gtaccagcca
     2461 gatgaatacc aattctgtgg atgttcgtta tcgcaatatc ccgttatggg atgatgcgac
     2521 attatcatta atggctaaat attccgcacc taataaaacg gatcaacaac aagataacga
     2581 aaatgacaac agttattttg aaatgaaaga tagctggatg ctgacttctg ttttacggca
     2641 aaacctgcaa cgcgatacgt ttaatgaatt tacgttacag gttgccaata attcctatgc
     2701 cagcagtttt gccagtttct cagatgccag taatacgatg gcgcatggtc gctattacta
     2761 tggtgatcat accaatggga tcgcctggcg tctaatctct cagggcgaga tgtatcttac
     2821 tgacaatatt attatggcta acgcgcttgt ctattctcac ggcgaagatg tttatagtta
     2881 tgaaagtggt gctcatagtg attttgacag tattcgcact gtaataagac cggcctgatc
     2941 tggaatacat ggaatcagac ggggcttgaa ttaggctggt ttaagcaaca gaacaaaact
     3001 cagcagggtg taacactaaa tgaatcggct tataaaacga cactctggca tgcattgaaa
     3061 gtgggtgaaa gcattttagg ttcacgacca gaaattcgct tctatggaac gtatatcaat
     3121 attctggata acgaattatc gaattttaaa tttaatgaga acagcaaaga cgagtttatg
     3181 gccggcatcc aggcggaagt ctggtggtaa tctattttag ttaaggagtt gtgtatgtca
     3241 ggatttaaaa aaggtttttt atggggcggc gcggtagccg cgcatcagtt ggaaggtggc
     3301 tggaatgaag gaggaaaagg catcagtatc gctgatgtga tgactgctgg cgctcacggg
     3361 gtgccgcgtg aagtgacaga aggcgttatc gacgggctta attatcccaa tcatgaagca
     3421 attgattttt atcatcgcta taaaacagat attcagttat ttgccgagat gggattcaaa
     3481 tgctttcgaa cttccattgc ctggacacga atctttccgc aaggtgacga acaggagccg
     3541 aatgaagagg gtttacaatt ttatgatgat ctgttcgatg aatgccttaa gcagggaatg
     3601 gaacctgtgg tgacgctttc acattttgag atgccttatc atctggtgac aaaatatggt
     3661 ggctggcgaa accgtaaact gatcgacttt tttatccgct tcgcatcaac ggtcttcacg
     3721 cgctataaag aaaaagtaaa gtactggatg acgtttaacg aaatcaataa tcaggtgaat
     3781 ttcagcgaaa gcctgtgtcc atttactaat tccggtatct tgtattcgcc agaggaagat
     3841 atcaatgagc gcgaacaaat aatgtaccag gcggtacatt acgagttagt tgccagtgcc
     3901 ctggcggtac agactggaaa atcgattaat cctgaattta atatcggctg catgatcgcc
     3961 atgtgcccca tctatcctct gacgtgtgca cccaacgata tgatgatggc gacgaaagcg
     4021 atgcatcgtc gttactggtt tactgatgtt catgctcgtg gatattatcc gcaacatatg
     4081 ctgaattact ttgccaggaa aggattcaac ctcgatatca caccagaaga taacgcgatt
     4141 cttgccagag gttgtgtcga ctttatcggt tttagctact acatgtcttt tacgacacaa
     4201 ttttcgcccg ataacccgca actggattat gttgaaccac gagatttggt cagcaaccct
     4261 tatatcgata catccgaacg gggatggcaa attgatccgg cagggctacg ttattcactc
     4321 aactggttgt gggatcattt ccagttgccg ctgtttattg tcgaaaatgg atttggtgcg
     4381 gttgaccaga gacaagctga cggcacagtg aacgatcact atcgcattga ttactttgct
     4441 tcccatattc gtgaaatgaa aaaagccgta gtcgaagatg gtgttgactt aattggctac
     4501 accccgtggg gctgcattga cctggtttct gccggaacag gggaaatgaa aaaacgctac
     4561 ggaatgattt atgtcgataa agacaacgaa gggaagggaa cgctggaaag gatacgtaaa
     4621 gcgtcgtttt actggtatcg ggatctcatc gccaacaatg gcgaaaatat ttgagacttc
     4681 agaaagatat tacggagatg tcaggctctg tgatcagagc ctgacaataa ctacatagcg
     4741 tgttgattat aatagggcac gccgagcgca tccgacttat cactgccagt acccatgtga
     4801 tttaaatcaa acggcggttg ttcctgcgat ggaggagcaa ccaccacatt gccacaggaa
     4861 gtaacaaccg gctacgtggg ttgttccgca acgccctgcg cggaaaagag aataagcgca
     4921 gctgctattg cggataaaag tggtttcatg attgcctcag tgacgttgtc acgttatcaa
     4981 ctagctgttg taatgattta atggatgtaa aaagcgcgat tcgccctgca tattggtcat
     5041 ccggtattta tgcggcggaa catcaaagta atttttgaag gttcgggtca gagtttgttg
     5101 cgactcgaag ccatatcgtt ctgccagata gagtatcggc tcgttacttt ccttcagctt
     5161 ttgcgcgatt tccgtcatct tacggctgcg gatgtattgg cctaatgaat gaccggtttc
     5221 ttttttaaac atccgttgca ggtgccattt ggagtaaccc gaacgctctg acactttctc
     5281 cagtgacagt ggcgattcca ggttgtcctc gatccagtcc aaaatgctat gaatggtaat
     5341 agcgtcagta ttgcgtctgg acatcgtcat acctcttttt tgtttacggc aggactttct
     5401 taagcaaatg ctcaagtgtt gccacttcgt ccgccgtcag gttttttgtt aattcttgat
     5461 gcaggtcctg gccaactaat tgatggcatt gttcgcatat tgccgcgccg ctggtggtaa
     5521 gttttaccag tacgccgcgc ttatcattcg ggttcggcaa ccgttcaacc cagcctttac
     5581 agaccaggcg atccagcata cgggtcagtg ctcccaggtc gaccgacaac acttttttca
     5641 gttcaaccgg agtaatacac gccgcgcagc ggatagagca gagcacctta aactgtgccg
     5701 cagtaatatc cagcggagac agatactcgt taagcagacg atctttcttc tgattaacca
     5761 tatggattaa gcgacccaat ggaataattt cattgaacag atcgctggta cttttcacat
     5821 tagttgccct ggcaagtaat tagttgcagg ggataatatt gcccaggcaa gtataagtca
     5881 aatgaatgaa tgaccgatgc cacgttttgc taaatcggtt caagttttgg tcaatgcgat
     5941 gcaaggttag ctaaagagtg acactttatc cttgtttacc ccttatactt ttcgctgata
     6001 acccagatac acaggataac aaccaccaat gttagatttg tttaaagcaa ttggcctggg
     6061 gctggtggtg ttactgccgt tagctaaccc attaacaact gtcgcgttat ttcttggcct
     6121 ggcaggcaac atgaacagtg ccgaacgtaa tcgtcagtcg ttgatggcct cggtgtacgt
     6181 atttgccatc atgatggtgg cgtattacgc cgggcaactg gtgatggata catttggcat
     6241 ttcgattccc ggtctgcgaa ttgcaggcgg cttaatcgtc gcgtttatcg gttttcggat
     6301 gctctttccg caacagaaag cgattgattc accggaggcg aaaagcaagt cggaagagct
     6361 ggaagatgaa cccagtgccc atattgcctt tgtgccgcta gcaatgccaa gtaccgctgg
     6421 tccgggaacc atagcgatga ttattagctc ggcgtccaca gtgcgtcaga gttcaacctt
     6481 cgctgattgg gtactgatgg ttgcgccgcc gctgatcttt ttcctggtgg cggttatttt
     6541 gtggggaagc ctacgcagct ccggcgcaat tatgcggctg gtgggcaagg gtggcattga
     6601 agcgatctcc cgtctgatgg gattcctgct ggtatgtatg ggtgtgcagt ttattattaa
     6661 cggcatcctg gaaatcatta aaacgtacca ttaaaaaagg cccgaatggg cctttcaact
     6721 attgcgtctg ttcttcgagt gtcactggcc agcggcgaaa tataatgatt gaccatatta
     6781 acgcggcaaa agcaggcacc gcgcccacat aaccaatcat cgacattgac cagtgcaaac
     6841 tcacctgatt acctaccaac gcacccgccc cgattccaat attaaatatg ccggagaata
     6901 gcgccatcgc gacgtcggta gcatctggtg ccagcgccag cactttaacc tgcataccaa
     6961 gcccgatgat catcatcgcg atcccccaga aaatactcag caccccgagg tgtatttcac
     7021 tgttcgccgc aggtagcagc agtgccagac acaccagcaa cagcgcaatt gccgtactca
     7081 ccaacgcaga cgcatactga ttacccagtt taccgaaaat cacgctgcca ataatgcccg
     7141 caccaccgag taataacagt aatgccgtgg caaagttggc gctgaatccc gcaatgtttt
     7201 gcacaaaagg ctcgatatag ctgtatgccg tgtaatgggc ggtgacaacc accacagtta
     7261 acaaataaat gctcatcaat gccgggcggc ggaacaatag cgggaggctt ttcagtgaac
     7321 cggaatgctc actgggcagt aagggaagta acttaatcag gcacaaaagg gtgataagcg
     7381 ccccaatacc aatcgcgaag aaggtcattc gccaaccgaa atactggccc acaatgcgcc
     7441 cgagaggtaa acctaagacc atcgccagtg ctgtaccggt ggcaattaaa ctcaatgcct
     7501 gtgctcgctt cccggccgga gccatacgga tcgccagaga cgccgtaatc gaccagaaaa
     7561 tcgcatgtgc aaaagccaca ccaatgcgac tgatcaccag aacggtaaag ctccacgaca
     7621 aaaacgacag tacgtggctg gcaataaaca ccacaaacag gcagatcagt aatttgcgcc
     7681 gttcaacctg actggtcatt aacataaaag gcaatgacat tagcgctact acccatgcgt
     7741 aaatggtcaa catgatgccg acctgagcgg tttgcatgtg aaaactttgc gcaatgtcag
     7801 agagcaggcc aacagggaca aattcggtgg tgttgaagat gaaggcggcg actgccagcg
     7861 taacgacccg tagccacgcc actttgcggg aaacagtgtt tgttgtcata acagattatc
     7921 gggtgagttg cgaaaaagtg atgaaatgat cttaaaacgg ttaatgatgt gaatacaatg
     7981 catttgtgat ctggatcgcg ttttccgttt ctctgacgct gattctgttc cgcgtcagag
     8041 agtcggtaac atcacggttt aatgacgttc aggaaggtct ggcgttttgg cggttgcggg
     8101 tataggtggt ctgacagggg agcgaggcct gtttttcacc aggtcttcgt gagtaacttt
     8161 tggcggcgtt tcatttttta caaacagttt gagggtcctg cgcgccatat gtttgataat
     8221 tttatcatca cttaatgatg ctggcatcaa cacgtcattc agatcacttt cattgggatt
     8281 cataaattgg tgatagtgca ataacatatt ggcgaaaaat aaaaaaagtc ctgaaaatga
     8341 ggagattctt gttctgctaa aagggataaa aagcagatgc agacttcggg tagtacatgt
     8401 ttgttagaac ctaatgaatc catcaggctt tgcaataaat cctcttttaa ttcgccgtct
     8461 gttatgtcga gaatggcggt gatcaagtta ggatctttat tgacgaacca tctgtgaacc
     8521 tcagagttac tccataatcg ggggagtatg acaaaattac cgcgagagat aaaattcagg
     8581 acaagcaaat caaactgacg taagtaatct gcagcggtat ggtgatccgg aggcaggggt
     8641 tcaatatgtg gtgtatgcgc tcttttgatg ggaacgccaa ggttgatcat tggaaattgt
     8701 tcaataaaat cattcagaaa cagaaagagc acacgctcga cgccggtctg aaatacgcgc
     8761 cccatagtga caggctttgg gttgggagcg caaaaaa
//
LOCUS       DAPHBJ010000177         8688 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.179, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000177 DAPHBJ010000000
VERSION     DAPHBJ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.179"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(276..539)
                     /gene="rpsT"
                     /locus_tag="RHZ76_003673"
     CDS             complement(276..539)
                     /gene="rpsT"
                     /locus_tag="RHZ76_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDV2814243.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            642..860
                     /locus_tag="RHZ76_003674"
     CDS             642..860
                     /locus_tag="RHZ76_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HDV2814244.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            868..1809
                     /gene="ribF"
                     /locus_tag="RHZ76_003675"
     CDS             868..1809
                     /gene="ribF"
                     /locus_tag="RHZ76_003675"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDV2814245.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKLLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1852..4668
                     /gene="ileS"
                     /locus_tag="RHZ76_003676"
     CDS             1852..4668
                     /gene="ileS"
                     /locus_tag="RHZ76_003676"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDV2814246.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVNGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGDKCPRCWHYTRDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4668..5162
                     /gene="lspA"
                     /locus_tag="RHZ76_003677"
     CDS             4668..5162
                     /gene="lspA"
                     /locus_tag="RHZ76_003677"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDV2814247.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5287..5736
                     /gene="fkpB"
                     /locus_tag="RHZ76_003678"
     CDS             5287..5736
                     /gene="fkpB"
                     /locus_tag="RHZ76_003678"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDV2814248.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5738..6688
                     /gene="ispH"
                     /locus_tag="RHZ76_003679"
                     /gene_synonym="lytB"
     CDS             5738..6688
                     /gene="ispH"
                     /locus_tag="RHZ76_003679"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDV2814249.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKSAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6754..7668
                     /gene="rihC"
                     /locus_tag="RHZ76_003680"
     CDS             6754..7668
                     /gene="rihC"
                     /locus_tag="RHZ76_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HDV2814250.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPHIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLVLVP"
     gene            7835..8656
                     /gene="dapB"
                     /locus_tag="RHZ76_003681"
     CDS             7835..8656
                     /gene="dapB"
                     /locus_tag="RHZ76_003681"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDV2814251.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
BASE COUNT         1981 a         2187 c         2463 g         2057 t
ORIGIN      
        1 cttaagttta atacctcaat gtgtatcaca atatccacat tctttgtagg gggtctggag
       61 attgagtaga tattcttgtt cagaatgtat cagccgatgg ttctacgatt cttaagccac
      121 gaagagttca gatagtacaa cggcatgtct gcaagaacct gcaacttttg actatctggc
      181 aaccggcagt gtgttctctc acgcatcaca aaagcagcag gcataaaaaa acccgcttgc
      241 gcgggctttt tcacaaagct tcagcaaatt ggcgattaag ccagtttgtt gatctgtgca
      301 gtcaggttag ccttatgacg tgcagctttg tttttgtgga tcagaccttt agcagcctga
      361 cggtccacga tcggttgcat ttcgttaaat gctttctgtg cagcagcttt gtcgccagct
      421 tcgatagctg cgtatacttt cttgatgaaa gtacgcatca tagagcgacg gcttgcgttg
      481 tgcttacgag ccttttcaga ctgaatggcg cgcttcttag ctgatttgat attagccaag
      541 gtccaactcc caaatgtgtt ctatatggac aattcaaagg ccgaggaata tgccctttta
      601 gccttctttt gtcaatggat ttgtgcaaat aagcgccgtt aatgtgccgg cactcgttac
      661 gtagtgatgg cgcaggattc taccagcttg cggggtgtga atacagcttt tccgcgataa
      721 aaattgcagc aggcggtcag tttcttcccg tgatttgcgc catggcaatg aaaagccact
      781 tctttctgat ttcggtactc aatcgccggt taaccttgac cgctgtacaa ggtatactcg
      841 gacgattttc actgttttga gccagacatg aagctgatac gcggcataca taatctcagc
      901 caggccccgc aagaagggtg tgtgctgact attggtaatt tcgacggcgt gcatcgcggt
      961 catcgcgcgc tgttacaggg cttgcaggaa gaagggcgca agcgcaactt accggtgatg
     1021 gtgatgcttt ttgaacctca accactggaa ctgtttgcta ccgataaagc cccggcacgg
     1081 ctgacccggc tgcgggaaaa actgcgttac cttgcagagt gtggcgttga ttacgtgctg
     1141 tgcgtgcgtt tcgacaggcg tttcgcggcg ttaaccgcgc aaaatttcat cagcgatctt
     1201 ctggtgaagc ttttgcgcgt aaaatttctt gccgtaggtg atgatttccg ctttggcgct
     1261 ggtcgtgaag gcgatttctt gttattacag aaagctggca tggaatacgg cttcgatatc
     1321 accagtacgc aaactttttg cgaaggtggc gtgcgtatca gcagcaccgc tgtgcgtcag
     1381 gcgctggcgg atgacaatct ggctctggca gaaagtttac tggggcaccc gtttgccatc
     1441 tccgggcgtg tagtccacgg tgatgaatta gggcgcacta taggtttccc gacggcgaat
     1501 gtaccgctac gccgtcaggt ttccccggtg aaaggggttt atgcggtaga agtgctgggc
     1561 ctcggtgaaa agccgttacc cggcgtggca aacatcggaa cacgcccaac ggttgccggt
     1621 attcgccagc agctggaagt gcatttgtta gatgttgcaa tggaccttta cggtcgccat
     1681 atacaagtag tgctgcgtaa aaaaatacgc aatgagcagc gatttgcgtc gctggacgaa
     1741 ctgaaagcgc agattgcgcg tgatgaatta accgcccgcg aattttttgg gctaacaaaa
     1801 ccggcttaag cctgttatgt aatcaaaccg aaacacggaa ccgagaatct gatgagtgac
     1861 tataaatcaa ccctgaattt gccggaaaca gggttcccga tgcgtggcga tctcgccaag
     1921 cgcgaaccgg gaatgctggc gcgttggact gatgatgatc tgtacggcat catccgtgcg
     1981 gctaaaaaag gcaaaaaaac cttcattctg catgatggcc ctccttatgc gaatggcagc
     2041 attcatattg gtcactcggt taacaagatt ctgaaagaca ttatcgtgaa gtccaaaggg
     2101 ctttccggtt atgactcgcc gtatgtgcct ggctgggact gccacggtct gccgatcgag
     2161 ctgaaagtcg agcaagaata cggtaagccg ggtgagaaat tcaccgccgc cgagttccgc
     2221 gccaagtgcc gcgaatacgc ggcgacccag gttgacggtc aacgcaaaga ctttatccgt
     2281 ctgggcgtgc tgggcgactg gtcgcacccg tacctgacca tggacttcaa aactgaagcc
     2341 aacatcatcc gcgcgctggg caaaatcatc ggcaacggtc acctgcacaa aggcgcgaag
     2401 ccggtacact ggtgcgttga ctgccgttct gcgctggcgg aagcggaagt tgagtattac
     2461 gacaaaactt ctccgtccat cgacgttgct tttcaggcgg tcgatcagga tgcactgaaa
     2521 gcaaaatttg ccgtaagcaa cgttaacggc ccaatctcgc tggtgatctg gaccactacg
     2581 ccgtggactc tgcctgcgaa ccgcgcaatc tctattgcac ctgatttcga ctatgcgctg
     2641 gtgcagatcg acggtcaggc cgtgattctg gcgaaagatc tggttgaaag cgtaatgcag
     2701 cgtatcggcg tgaccgatta caccattctc ggcacggtaa aaggtgcgga gcttgagttg
     2761 ctgcgcttta cccatccgtt tatgggcttc gacgttccgg caatcctcgg cgatcacgtt
     2821 accctggatg ccggtaccgg tgccgttcac accgcgcctg gccacggccc ggacgactat
     2881 gtgatcggtc agaaatacgg cctggaaacc gctaacccgg ttggcccgga cggcacttat
     2941 ctgccgggca cttatccgac gctggatggc gtgaacgtct tcaaagcgaa cgacatcgtc
     3001 gttgcgctgc tgcaggaaaa aggcgcgctg ctgcacgttg agaaaatgca gcacagctat
     3061 ccgtgctgct ggcgtcacaa aacgccgatc atcttccgcg cgacgccgca gtggttcgtc
     3121 agcatggatc agaaaggtct gcgtgcgcag tcactgaaag agatcaaagg cgtgcagtgg
     3181 atcccggact ggggccaggc gcgtatcgag tcgatggttg ctaaccgtcc tgactggtgt
     3241 atctcccgtc agcgcacctg gggcgtaccg atgtcactgt tcgtgcacaa agacacggaa
     3301 gagctgcatc cgcgtaccct cgaactgatg gaagaagtgg caaaacgcgt tgaagttaac
     3361 ggcatccagg cgtggtggga tctcgatgcg aaagagatcc tcggcgacga agctgaccag
     3421 tatgtgaaag tgccggatac tctcgacgta tggtttgact ccggatctac ccactcttct
     3481 gttgttgacg tgcgtccgga atttgccggt cacgcagcgg acatgtatct ggaaggttct
     3541 gaccagcacc gtggttggtt catgtcttcc ctaatgatct ccaccgcgat gaaaggcaaa
     3601 gcgccgtatc gtcaggtact gacccacggc tttaccgtgg atggtcaggg ccgcaagatg
     3661 tctaaatcca tcggcaacac cgtttcgccg caggatgtga tgaacaaact gggcgcggat
     3721 attctgcgtc tgtgggtggc atcaaccgac tacaccggtg aaatggccgt ttctgacgag
     3781 atcctgaaac gtgctgccga cagctatcgt cgtatccgta acaccgcgcg cttcctgctg
     3841 gcaaacctga acggttttga tccagcaaaa gatatggtga aaccggaaga gatggtggta
     3901 ctggatcgct gggccgtagg ttgtgcgaaa gcggcacagg aagacatcct caaggcgtac
     3961 gaagcatacg atttccacga agtggtacag cgtctgatgc gcttctgctc cgttgagatg
     4021 ggttccttct acctcgacat catcaaagac cgtcagtaca ccgccaaagc ggacagcgtg
     4081 gcgcgtcgta gctgccagac tgcgctgtat cacatcgcag aagcgctggt tcgctggatg
     4141 gcaccaatcc tctccttcac cgctgatgaa gtgtggggct acctgccggg cgaacgtgaa
     4201 aaatacgtct tcaccggtga gtggtacgaa ggcctgtttg gtctggcaga cagtgaagca
     4261 atgaacgatg cgttctggga cgagctgttg aaagtgcgtg gcgaagtgaa caaagtcatt
     4321 gagcaagcgc gtgccgacaa gaaagtgggt ggctcgctgg aagcggcagt aaccttgtat
     4381 gcagaaccgg aactggcggc gaaactgacc gcgctgggcg atgaattacg atttgtcctg
     4441 ttgacctccg gcgctaccgt tgcagactat aacgacgcac ctgctgatgc tcagcagagc
     4501 gaagtactca aagggctgaa agtcgcgttg agtaaagccg aaggtgataa gtgcccacgc
     4561 tgctggcact acacccggga tgtcggcaag gtggcggaac acgcagaaat ctgcggccgc
     4621 tgtgtcagca acgtcgccgg tgacggtgaa aaacgtaagt ttgcctgatg agtcaatcga
     4681 tctgttcaac agggctacgc tggctgtggc tggtggtagt cgtgctgatt atcgatctgg
     4741 gcagcaaata cctgatcctc cagaactttg ctctggggga tacggtcccg ctgttcccgt
     4801 cgcttaatct gcattatgcg cgtaactatg gcgcggcgtt tagtttcctt gccgatagcg
     4861 gcggctggca gcgttggttc tttgccggta ttgcgattgg tattagcgtg atcctggcag
     4921 tgatgatgta tcgctcgaag gccacgcaga agctaaacaa tatcgcttat gcgctgatta
     4981 ttggcggcgc gctgggcaac ctgttcgacc gcctgtggca cggcttcgtt gtcgatatga
     5041 tcgacttcta cgtcggcgac tggcacttcg ccaccttcaa ccttgccgat actgccattt
     5101 gtgtcggtgc ggcgttgatc gtgctggaag ggtttttacc ttctaaagcg aaaaaacaat
     5161 aataaaccct gccggatgcg atgctgacgc atcttatccg gcctacagat tgctgcgaaa
     5221 tcgtaggccg gataaggcgt ttacgccgca tccggcaaaa atcctcaaat ataagagcaa
     5281 acctgcatgt ctgaatctgt acagagcaat agcgccgtcc tggtgcactt cacgctaaaa
     5341 ctcgacgatg gcaccaccgc cgagtctacc cgcaacaacg gtaaaccggc gctgttccgc
     5401 ctgggcgatg cttctctttc tgaagggctg gagcaacacc tgctggggct gaaagtgggc
     5461 gataaaacca ccttctcgct ggagccagat gcggcgtttg gcgtgccgtc accggacctg
     5521 attcagtact tctcccgccg tgagtttatg gatgcaggcg agccagaaat tggcgcaatc
     5581 atgcttttta ccgcaatgga tggcagtgag atgcctggcg tgatccgcga aattaacggc
     5641 gactccatta ccgttgattt caaccatccg ctggccgggc agaccgttca ttttgatatt
     5701 gaagtgctgg aaatcgatcc ggcactggag gcgtaacatg cagatcctgt tggccaaccc
     5761 gcgtggtttt tgtgccgggg tagaccgcgc tatcagcatt gttgaaaacg cgctggccat
     5821 ttacggcgca ccgatatatg tccgtcacga agtggtgcat aaccgctacg tggtcgatag
     5881 cctgcgcgag cgtggggcta tctttattga gcagattagc gaagtgccgg acggcgcaat
     5941 cctgattttc tccgcacacg gtgtttctca ggcggtacgt aacgaagcga aaagccgtga
     6001 tttgacggta ttcgacgcca cctgcccgct ggtgaccaaa gtgcatatgg aagtcgcccg
     6061 cgctagtcgc cgtggcgaag aatctattct catcggtcac gccgggcacc cggaagtgga
     6121 aggcacgatg ggtcagtaca gcaacccgga agggggaatg tatctggttg aatcaccaga
     6181 cgatgtgtgg aaactgacgg tcaaaaacga agagaaactc tcctttatga cccagaccac
     6241 gctgtcggtg gatgacacgt ctgatgtgat cgacgcgctg cgtaaacgct tcccgaaaat
     6301 tgtcggtccg cgcaaagatg acatctgcta cgccacgact aaccgacagg aagcggtacg
     6361 tgccctggcc gaacaggcgg aagttgtgct ggtggtcggt tcgaaaaact cctctaactc
     6421 caaccgtctg gcggagctgg cccagcgtat gggcaaaagc gcgtttttga ttgatgatgc
     6481 gaaagatatc caggaagagt gggtgaaaga ggttaaatgc gtcggcgtga ctgcgggtgc
     6541 atcggctccg gatattctgg tgcagaatgt ggtggcacgt ttgcaacagc tgggcggtgg
     6601 tgaagccatt ccgctggaag gacgtgaaga aaacattgtt ttcgaagtgc cgaaagagct
     6661 gcgtgtcgat attcgtgaag tcgattaagt cattagcagc ctaagttatg cgaaaatgcc
     6721 ggtcttgtta ccggcatttt ttatggagaa aacatgcgtt tacctatctt cctcgatact
     6781 gaccccggca ttgacgatgc cgtcgccatt gccgccgcga tttttgcccc cgaactcgac
     6841 ctgcaactga tgaccactgt cgcgggtaat gtttcggtag agaaaaccac ccgcaatgcc
     6901 ctgcaactgc tgcatttctg gaatgcggag attccgctcg cccagggagc cgctgtgccg
     6961 ctggtacgcg caccgcgtga tgcggcatct gtgcacggcg aatcgggaat ggctggctac
     7021 gactttgttg agcacaaccg aaagccactc gggataccgg cgtttctggc gattcgggat
     7081 gccctgatgc gtgcaccaga gcctgttact ctggtagcca tcggcccgtt aaccaatatt
     7141 gcgctgttac tttcacaatg cccggaatgc aaaccgcata ttcgccgtct ggtgatcatg
     7201 ggtggttctg ccggtcgcgg caactgcaca ccaaacgccg agtttaatat cgctgccgat
     7261 ccagaagcgg ctgcctgtgt cttccgtagc ggaatcgaaa ttgtcatgtg cggtctggat
     7321 gtcaccaatc aggcaatatt aacccctgac tatctcgcta ctttgccgga gttgaatcgc
     7381 accggaaaaa tgctccacgc cctgtttagc cactaccgta gcggcagtat gcaaagcggt
     7441 ctgcgaatgc acgatctctg cgccatcgcc tggctggtgc gcccggactt attcaccctc
     7501 aagccgtgtt ttgtcgcggt agaaactcaa ggcgaattta cctcaggcac gacggtggtt
     7561 gatatcgacg gttgcttggg caaaccagcc aatgtacagg tggcattgga tctgaatgta
     7621 aaaggcttcc agcagtgggt agctgaggtg ctggttctgg tgccgtaacc tgtcacatgt
     7681 tatcggcatg cagtcattca tcgactcatg cctttcactg atatccctcc ctgtttatca
     7741 ttaatttcta attatcagcg tttttggctg gcggcgtagc gatgcgctgg ttactctgaa
     7801 aacggtctat gcaaattaac aaaagagaat agctatgcat gatgcaaaca tccgcgttgc
     7861 catcgcggga gccggggggc gtatgggccg ccagttgatt caggcggcgc tggcattaga
     7921 gggcgtgcag ttgggcgctg cgctggagcg tgaaggatct tctttactgg gcagcgacgc
     7981 cggtgagctg gccggagccg ggaaaacagg cgttaccgtg caaagcagcc tcgatgcggt
     8041 aaaagatgat tttgatgtgt ttatcgattt tacccgtccg gaaggtacgc tgaaccatct
     8101 cgctttttgt cgccagcatg gcaaagggat ggtgatcggc actacggggt ttgacgaagc
     8161 cggtaaacaa gcaattcgtg acgccgctgc cgatattgcg attgtctttg ctgccaattt
     8221 tagcgttggc gttaacgtca tgcttaagct gctggagaaa gcagccaaag tgatgggtga
     8281 ctacaccgat atcgaaatta ttgaagcaca tcatagacat aaagttgatg cgccgtcagg
     8341 caccgcactg gcaatgggag aggcgatcgc ccacgccctt gataaagatc tgaaagattg
     8401 cgcggtctac agtcgtgaag gccacaccgg tgaacgtgtg cctggcacca ttggttttgc
     8461 caccgtgcgt gcaggtgaca tcgttggtga acataccgcg atgtttgccg atattggcga
     8521 gcgtctggag atcacccata aggcgtccag ccgtatgaca tttgctaacg gcgcggtaag
     8581 atcggctttg tggttgagtg gtaaggaaag cggtcttttt gatatgcgag atgtacttga
     8641 tctcaataat ttgtaaccac aaaatatttg ttatggtgca aaaataac
//
LOCUS       DAPHBJ010000178         8662 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.180, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000178 DAPHBJ010000000
VERSION     DAPHBJ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.180"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(106..726)
                     /gene="entD"
                     /locus_tag="RHZ76_003682"
     CDS             complement(106..726)
                     /gene="entD"
                     /locus_tag="RHZ76_003682"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HDV2814252.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(901..3141)
                     /gene="fepA"
                     /locus_tag="RHZ76_003683"
     CDS             complement(901..3141)
                     /gene="fepA"
                     /locus_tag="RHZ76_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HDV2814253.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            3384..4586
                     /gene="fes"
                     /locus_tag="RHZ76_003684"
     CDS             3384..4586
                     /gene="fes"
                     /locus_tag="RHZ76_003684"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HDV2814254.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTNVWQWKTQLNANWRGSYCFIPTERDDIF
                     SVPSPDRLELREGWRKLLPQAIADPLNLQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKKSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            4589..4807
                     /gene="ybdZ"
                     /locus_tag="RHZ76_003685"
     CDS             4589..4807
                     /gene="ybdZ"
                     /locus_tag="RHZ76_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HDV2814255.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWLQQCVLPAGWDIVCQPQ
                     SQASCQQWLEAHWRTLTPTNFTQLQEAQ"
     gene            4804..>8662
                     /gene="entF"
                     /locus_tag="RHZ76_003686"
     CDS             4804..>8662
                     /gene="entF"
                     /locus_tag="RHZ76_003686"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HDV2814256.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSELPSAWSVAHYVELTGEVDSPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLAMQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQLNATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQVARQVTPGQVM
                     VASTVAKLATIIDAEEDSTRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPNGPMQTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGTFEKIGPI"
BASE COUNT         1869 a         2340 c         2384 g         2069 t
ORIGIN      
        1 aatccccgca tatccggttg tcaggtcagg atggtaaggc acctgcttta cactttcgcc
       61 cgtggtcagt gatggctgcg ggcgaatcgt accagatgtt gtcaattaat cgtgttggca
      121 cagcgttatg actatctttt cttttatctg ccagtgcaca gcaaacatct cattctcacg
      181 atgaatgatg acctgctgtt tattccagct aattatctga tagtccagaa aacctgcatc
      241 agtttggatc tcacttgcct taaatgcgct ctctttggcg gaaaatgcca gtgtcagcgc
      301 cagagaaaag gctaaaccgc agtctgcgag tcgctcgtgt tctgctggtg taataatgtt
      361 gtctgtcaat tcggtcgctg tttgcgcaga aaaaatttct tcgatatcaa cgccaatcgg
      421 ttgacgggaa accacggcca atgccgtagc cccgcagtgg ctaatactgc cgtatacctc
      481 cgcaggccac accggttggc gtagctcgcc gattgcgggc acacatttat agccatattc
      541 ccgcaaagca taaacagcag cgatccgtcc ggctaaatgc tctgttttac gtttacgtcc
      601 agcgtgttgc agttgtgcgt agtgcggcag ccagagtaaa tcctgctcac aaaaattcgc
      661 cggatcgaac tcaacaaaat gcagcgtatg tccggcaaag gggagggagg tatgcgtagt
      721 tttcatatcg accatattcg agactgatga caaacgcaaa actgcctgat gcgctacgct
      781 tatcaggcct acatggtcct gcaatatatt gaattggcaa gatttttgta ggccggataa
      841 ggcgttcacg ccgcatccgg catgaacgac gcgcactttg tcaacaatct gacgttagca
      901 tcagaagtga gtgtttacgc tcatatacca cgtacgtccc ggctcgttat aggtatacgc
      961 cccggcaccg gcgatatagt tggcccctgc caaatcgccc gtggtctggg cattacccgc
     1021 acgccacaaa cgtttgtcga acagattgtc cacgccgccg gtcagactga cattcttcgt
     1081 cacatcccag gtcgcgctca ggccaacaat gctgtaagga ctaatttctt tggtttccgg
     1141 tccaaccgct ggctgacctt tatagttgta cttcttcggc tgctgcttgc cgtaccaggt
     1201 gaaggtcgtt tgcatcgaca aatcttcccg tgcctgccag ctcagcgttg agttcaacgt
     1261 atactccggg atgatcgaca aacggtcgcc cgtggttttg ttttcactct tcagcatata
     1321 agtgatgtta ttggtccaca tcaccgtttc gctaaccggt acgtttaacg atccttccag
     1381 accttcaacc accgctttcg gcacgttatc ccactgatag agatcggtgc cgactgcgtt
     1441 ttgccctaca gccacatagc ccgcttcaat cttattgcga taatcgttac ggaaccaggt
     1501 gacgcccgcc agccagccgt cgcgtttgaa ctccagacca atctctttgt tgatgctggt
     1561 ttctgctttc aggtcatcgt taccttgcag atagcagccg cccgcgctgg catagcaacc
     1621 ctgaccttta ctgtagagaa tgtagttcgg gttagtctgg tacaggctcg gcgctttata
     1681 agcacgggcg atgcccattt tcagcgtgaa gtcatcgcct aaaccttgcg atatgttcag
     1741 cgccgggctc cagttattgc cgacaatact gtgatgatcg aaacgcagcc ccggcgttac
     1801 gatggtgctg tcagtcagct ccatgttgtt ttcggcaaac agcgagaaaa tttctgcttt
     1861 tgaatacggg ctacggtcgg tggcactcac gccatcaata gcgccaccgg tattcgttcc
     1921 ggtcagtgcc tgggtgttgg aacttaagtc cttcatccgt tgctgattcc actccgtacc
     1981 cagcgtcagc gtctggttaa cgaggaaatc aatcggcagg ttaacttcgc tatgcagcat
     2041 cacgtcatca agatcgatat cgacgaaatc ctgtgtcgct ttttcgttaa atttcccttc
     2101 ggtaccgccc gccagacctt ccggaatacg cgagttacgg gtgtgttcgt actgcaccca
     2161 gttgctggtg gtcacgccgt tatcccagcc accgttccag gtcagcgcgt agttctggcg
     2221 atacagacgg ttggtttcat cgccatattt cgagcgggta taggaatcgg agttggtatt
     2281 ctgggtgtcg cccgcataca ggttaccctg gcggctgtaa cctgcttcca gttccagcga
     2341 ttgcaatggc gcgaaatccc agcgcaccac gccattaata tctttgttga ttaccccttc
     2401 gcgcccggct ggtaacgtcg tggcatacgt tccggcacgc gcggactgat ggccctggtt
     2461 gatatcccac gcgtcagcct gggttttgtc gaggttgcca tacaaacgga agctgaattc
     2521 gtcgcccagc ggaccggtca ggctaaagtt agtgcgtttg gtggcacctt cctctttatg
     2581 ttctggcgca ttgaaatatg cgtcccagga gccgtgccac tcgccgctgc cttttttggt
     2641 aatgatgtta accacgccgc ccgccgcgcc gttgccataa cgcgcagctg ccggaccacg
     2701 cagaacttca atacgttcaa tcatttcagg tggcacccag gaagtatcac cacgggtatc
     2761 gcgctcgcca cgccagccct gacgcaccga gttacggctg cttaccggct tgccgtcaat
     2821 caaaatcagc gtgttttccg gacccatacc gcgaatatca atctgtcggt tattcccacg
     2881 ctgaccactg gtggagttac cggtcaggtt aacgcctggc atggtacgga tgatctccga
     2941 cacatcgcgg gcaaccgggt ttttgcggat ttcatctgcg gtgatggtcg aaacgccagg
     3001 cgcctgtaag ttctgctcgg cggcggtaac gacaatagta tcgtcatgtg aaacaggagt
     3061 atcggtcggc tcttgtgcct gcgctacccc ataaatcccc agattgacca acaaggccag
     3121 ggaatgaatc ttcttgttca ttgttttatt cctgcatttt tgccacgaat tgcaactgtc
     3181 gggcatggtc gtcatcaaca cgacgcatcc cgctaccgcg aaaacctttg atcctgaaag
     3241 acacgcagtg cagttggtta attaatgtcc gcgcttccca tggcgcgcca ttacgctatt
     3301 gcaaatgcaa atagttatca ataatattat caatatattt ctgcaatcaa tgaaaaattg
     3361 cacagtaaac atggggttat ggtgtgacgg cgttaaaagt aggaagtgag agctggtggc
     3421 agtcgaaaca tggcccggaa tggcagcgtc tgaatgacga aatgtttgag gtcactttct
     3481 ggtggcgtga tccccaaggt tctgaagaat actcgacgat aaagcgcgta tgggtctaca
     3541 tcactggtgt gaccgatcac catcagaaca gccagcccca gtcgatgcag cgaattgcag
     3601 gcactaacgt ctggcagtgg aagacacaac tcaatgccaa ctggcgcggc agctactgct
     3661 ttattcccac cgaacgcgat gacatttttt ctgtaccatc ccccgatcgc ctcgaattgc
     3721 gcgaaggctg gcgaaaacta ttaccccagg cgatagccga tccgctgaac ctacaaagct
     3781 ggaaaggcgg gcgagggcac gctgtttctg cactcgaaat gccgcaagcg cctctgcaac
     3841 cgggatggga ttgtccgcaa gcgccagaaa tacctgccaa agaaattatc tggaaaagtg
     3901 aacggttgaa aaagtcacgg cgtgtatgga tttttaccac cggcgatgca acagcagaag
     3961 aacgcccgct ggcagttttg ctcgatggcg aattttgggc gcaaagtatg cccgtctggc
     4021 cagtgctgac ttcgctgacc catcgtcagc aacttcctcc cgccgtgtat gtgttgatcg
     4081 acgctatcga caccacgcac cgcgcccacg aactgccgtg taatgcggat ttctggctcg
     4141 cagtacagca agagttatta cccctggtga aagctattgc cccttttagc gatcgtgccg
     4201 atcgcaccgt ggttgccggg cagagttttg gtgggctttc cgcgctgtat gccggactgc
     4261 actggcctga acgctttggc tgtgtattaa gccagtcagg atcgtactgg tggccgcatc
     4321 ggggcgggca gcaagagggc gtgttacttg aaaagctaaa agctggtgaa gttagcgccg
     4381 aaggtctgcg cattgtgctg gaagcgggta ttcgcgagcc gatgatcatg cgggccaatc
     4441 aggcgctgta tgcgcaatta caccccataa aagaatccat tttctggcgt caggttgacg
     4501 gcggacatga tgcgctttgt tggcgcggtg gcttgatgca ggggctaatc gacctctggc
     4561 aaccactttt ccatgacagg agttgaatat ggcattcagt aatcccttcg atgatccgca
     4621 gggagcgttt tacatattgc gcaatgcgca ggggcaattc agtctgtggc tgcaacaatg
     4681 cgtcttaccg gcaggctggg acattgtgtg tcagccgcag tcacaggcgt cctgccagca
     4741 gtggctggaa gcccactggc gtactctgac accgacgaat tttacccagt tgcaggaggc
     4801 acaatgagcc agcatttacc tttggtcgcc gcacagcccg gcatctggat ggcagaaaaa
     4861 ctgtcagaat taccctccgc ctggagcgtg gcgcattacg ttgagttaac cggagaggtt
     4921 gattcgccat tactggcccg cgcggtggtt gccggactag cgcaagcaga tacgctgcgg
     4981 atgcgtttta cggaagataa cggcgaagtc tggcagtggg tcgatgatgc gctgacgttc
     5041 gaactgccag aaattatcga cctgcgaacc aacattgatc cgcacggtac tgcgcaggca
     5101 ttaatgcagg ccgatttgca gcaagatctg cgcgtcgata gcggtaaacc actggtcttt
     5161 catcagctca ttcaggtggc agataaccgc tggtactggt atcagcgtta tcaccatttg
     5221 ctggtcgatg gctttagttt cccggccatt acccgccaga tcgccaatat ttactgcaca
     5281 tggctgcgtg gcgaaccaac gcctgcttcg ccgtttacgc ctttcgctga tgtagtggaa
     5341 gagtaccagc aataccgcga aagcgaagcc tggcagcgtg atgcggcatt ctgggcggaa
     5401 cagcgtcgtc aactgccacc gcccgcgtca ctttctccgg cacctttacc ggggcgcagc
     5461 gcctcggcag atattctgcg cctgaaactg gaatttaccg acggggaatt ccgccagctg
     5521 gctatgcaac tttcaggtgt gcagcgtacc gatttagccc ttgcgctggc ggctttgtgg
     5581 ctggggcgat tgtgcaaccg catggactac gctgccggat ttatctttat gcgtcgactg
     5641 ggctcggcgg cgttgacggc taccggaccc gtgctcaacg ttttgccgtt gggtattcat
     5701 attgcggcgc aagaaacgct gccggaactg gcaacccgac tggcggctca actgaaaaaa
     5761 atgcgtcgtc atcaacgtta cgatgccgaa caaattgtcc gtgacagcgg gcgagcggca
     5821 ggtgatgaac cgctgtttgg tccggtactc aatatcaagg tatttgatta ccaactggat
     5881 attcctggtg ttcaggcgca aacccatacc ctggcaaccg gtccggttaa tgaccttgaa
     5941 ctggccctgt tcccggatga acacggtgat ttgagtattg agatcctcgc caataaacag
     6001 cgttacgatg agccaacgtt aatccagcat gctgaacgcc tgaaaatgct gattgcccag
     6061 ttcgccgcgg atccggcgct gttgtgcggc gatgtcgata ttatgctgcc aggtgagtat
     6121 gcgcagctgg cgcagctcaa cgccactcag gttgagattc cagaaaccac gcttagcgcg
     6181 ctggtggcag aacaagcggc aaaaacaccg gatgctccgg cgctggcaga tgcgcgttac
     6241 ctgttcagct atcgggaaat gcgcgagcag gtggtggcgc tggcgaatct gctgcgtgag
     6301 cgcggcgtta aaccagggga cagcgtggcg gtggcactac cgcgctcggt ctttttgacc
     6361 ctggcactcc atgcgatagt tgaagctgga gcggcctggc taccgctgga taccggctat
     6421 ccggacgatc gcctgaaaat gatgctggaa gatgcgcgtc cgtcgctgtt aattaccacc
     6481 gacgatcaac tgccgcgctt tagcgatgtt cccaatttaa caagcctttg ctataacgcc
     6541 ccgcttacac cgcagggcag tgcgccgctg caactttcac aaccgcatca cacggcttat
     6601 atcatcttta cctctggctc caccggcagg ccgaaagggg taatggtcgg gcagacggct
     6661 atcgtcaacc gcctgctttg gatgcaaaat cattatccgc ttacaggcga agatgtcgtt
     6721 gcccaaaaaa cgccgtgcag ttttgatgtc tcggtgtggg agtttttctg gccgtttatc
     6781 gcaggggcaa aactggtgat ggctgaaccg gaagcgcacc gcgacccgct cgctatgcag
     6841 caattctttg ccgaatatgg cgtaacgacc acgcactttg tgccgtcgat gctggcggca
     6901 tttgttgcct cgctgacgcc gcaaaccgct cgccagagtt gcgcgacgtt gaaacaggtt
     6961 ttctgtagtg gtgaggcctt accggctgat ttatgccgcg aatggcaaca gttaactggc
     7021 gcgccgttgc ataatctata tggcccgacg gaagcggcgg tagatgtcag ctggtatccg
     7081 gcttttggcg aggaactggc acaggtgcgc ggcagcagtg tgccgattgg ttatccggta
     7141 tggaatacgg gtctgcgtat tcttgatgcg atgatgcatc cggtgcctcc gggtgtggcg
     7201 ggtgatctct atctcactgg cattcaactg gcgcagggct atctcggacg ccccgatctg
     7261 accgccagcc gctttattgc cgatcctttt gccccaggtg aacggatgta ccgtaccgga
     7321 gacgttgccc gctggctgga taacggcgcg gtggagtacc tcgggcgcag tgatgatcag
     7381 ctaaaaattc gcgggcagcg tatcgaactg ggcgaaatcg atcgcgtgat gcaggcgctg
     7441 ccggatgtcg aacaagccgt tacccacgcc tgtgtgatta accaggcggc tgccaccggt
     7501 ggtgatgcgc gtcaattggt gggctatctg gtgtcgcaat cgggcctgcc gttggatacc
     7561 agcgcattgc aggcgcagct tcgtgaaaca ttgccaccac atatggtacc ggtggttctg
     7621 ctgcaacttc cacagttacc acttagcgcc aacggcaagc tggatcgcaa agccttaccg
     7681 ttgcctgaac tgaaggcaca agcgccaggg cgtgcgccga aagcgggcag tgaaacgatt
     7741 atcgccgcgg cattctcgtc gttgctgggg tgtgacgtgc aggatgccga tgctgatttc
     7801 ttcgcgcttg gcggtcattc gctactggca atgaaactgg cagcgcagtt aagtcggcag
     7861 gttgcccgcc aggtgacgcc ggggcaagtg atggtcgcgt caactgtcgc caaactggca
     7921 acgattattg atgctgaaga agacagcacc cggcgtatgg gattcgaaac cattctgccg
     7981 ttgcgtgaag gtaatggccc gacgctgttt tgtttccatc ctgcgtccgg ttttgcctgg
     8041 cagttcagcg tgctctcgcg ttatctcgat ccacaatggt cgattatcgg cattcagtca
     8101 ccgcgcccca atggccccat gcagacggcg gcaaacctgg atgaagtctg cgaagcgcat
     8161 ctggcaacgt tacttgaaca acaaccgcat ggcccttatt acctgctggg gtattccctt
     8221 ggcggtacgc tggcgcaggg tattgcggcg cgactgcgtg cccgtggcga acaggtggca
     8281 tttcttggct tgctggatac ctggccgcca gaaacgcaaa actggcagga aaaagaagct
     8341 aatggtctgg acccggaagt gctggcggag attaaccgcg aacgcgaggc cttcctggca
     8401 gcacagcagg gaagtacttc aacggagttg tttaccacca ttgaaggcaa ctacgctgat
     8461 gctgtgcgcc tgctgacgac tgctcatagc gtaccgtttg acggtaaagc gacgctgttt
     8521 gttgctgaac gcacacttca ggaaggtatg agtcccgaac gcgcctggtc gccgtggata
     8581 gcggagctgg atatctatcg tcaggattgt gcgcatgtgg atattatctc tccagggacg
     8641 tttgaaaaaa ttgggccgat ta
//
LOCUS       DAPHBJ010000179         8633 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.181, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000179 DAPHBJ010000000
VERSION     DAPHBJ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.181"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            26..676
                     /locus_tag="RHZ76_003687"
     CDS             26..676
                     /locus_tag="RHZ76_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 domain-containing protein"
                     /protein_id="HDV2814257.1"
                     /translation="MREMNQLVNTEDSAWPIIQNWLKDATNHTELLPVNKDLAETALY
                     QLQVTTKSPMGALVYGSGGLLIDNGWLRIAGSGHPRLPRDPVSWTQRPEFAGVRALPI
                     ADDVAGGIFALNGGDLGEDTGCVYYFAPDTLNWESLEVGYSEFLQWALSGDLDTFYEN
                     VRWQQWREDVIKLSATEAFTFYPFLWVQSEEARTRKVISLTELWEMQYQMKETFTQ"
     gene            complement(717..1211)
                     /locus_tag="RHZ76_003688"
     CDS             complement(717..1211)
                     /locus_tag="RHZ76_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814258.1"
                     /translation="MKTNRSLVVIVSLITATLLLTACAQPEQSSLAGDWLLTPKDKTR
                     GLTGSIAVNIAPFRCKTNCRGDNLPDNTRRWQLSGGNEKELTYLHNMSAQEKVGLNPG
                     WQCYTSFFMRVCQGKPGTRPIVNEDYVSESGFFGSMMHVGIIELRRCQSENCQQELKA
                     INTH"
     gene            complement(1425..3065)
                     /gene="pgm"
                     /locus_tag="RHZ76_003689"
     CDS             complement(1425..3065)
                     /gene="pgm"
                     /locus_tag="RHZ76_003689"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HDV2814259.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAVLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(3091..3636)
                     /gene="seqA"
                     /locus_tag="RHZ76_003690"
     CDS             complement(3091..3636)
                     /gene="seqA"
                     /locus_tag="RHZ76_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HDV2814260.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            3821..4585
                     /gene="ybfF"
                     /locus_tag="RHZ76_003691"
     CDS             3821..4585
                     /gene="ybfF"
                     /locus_tag="RHZ76_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDV2814261.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            4725..5018
                     /gene="ybfE"
                     /locus_tag="RHZ76_003692"
     CDS             4725..5018
                     /gene="ybfE"
                     /locus_tag="RHZ76_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HDV2814262.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            5158..5688
                     /gene="fldA"
                     /locus_tag="RHZ76_003693"
     CDS             5158..5688
                     /gene="fldA"
                     /locus_tag="RHZ76_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HDV2814263.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            5898..5984
                     /gene="uof"
                     /locus_tag="RHZ76_003694"
     CDS             5898..5984
                     /gene="uof"
                     /locus_tag="RHZ76_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HDV2814264.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            5977..6423
                     /gene="fur"
                     /locus_tag="RHZ76_003695"
     CDS             5977..6423
                     /gene="fur"
                     /locus_tag="RHZ76_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HDV2814265.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(6507..6833)
                     /locus_tag="RHZ76_003696"
     CDS             complement(6507..6833)
                     /locus_tag="RHZ76_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814266.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(6883..8289)
                     /gene="chiP"
                     /locus_tag="RHZ76_003697"
                     /pseudo
     CDS             complement(6883..8289)
                     /gene="chiP"
                     /locus_tag="RHZ76_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
BASE COUNT         2150 a         2138 c         2118 g         2227 t
ORIGIN      
        1 cgtagcacac aaggagcaag tagcgatgcg tgaaatgaat cagttagtaa atactgaaga
       61 ttcagcctgg ccaataatcc agaactggtt aaaagacgcc acaaaccata ccgaactctt
      121 accggttaat aaagatttag ccgaaaccgc actttatcaa ttacaggtga caacaaaatc
      181 accgatgggt gcgcttgtct atggctccgg aggtctgctg attgataatg gttggttacg
      241 catagcagga tctggtcacc cccggttgcc acgagatcca gtaagttgga cacaacgccc
      301 tgaattcgca ggcgtgcggg ctttacccat cgctgacgat gtcgcagggg gaatttttgc
      361 tcttaatggt ggcgatcttg gggaagatac tggctgcgtt tattattttg cgccagatac
      421 gctgaattgg gaaagtctgg aagttggata ttctgagttt cttcagtggg cattatccgg
      481 tgatttagat accttctatg aaaatgtacg atggcagcaa tggcgagaag acgtaataaa
      541 attatcggca acagaagcct tcacgttcta ccctttctta tgggtacaaa gtgaagaagc
      601 aagaacaaga aaagttattt cgcttactga actctgggaa atgcagtatc aaatgaaaga
      661 aacattcacg caataagaca tgttcaggtc cgggtttcat tcccggactc actattttaa
      721 tgtgtattta tcgctttcaa ttcttgctgg caattttctg actggcagcg tcgtaactca
      781 ataatcccga catgcatcat cgagccaaaa aatccgcttt cgctgacata atcctcattg
      841 actatcggtc gagtgccggg cttgccctgg caaacccgca taaaaaatga ggtgtagcat
      901 tgccaaccgg gatttaaacc gaccttttct tgtgctgaca tattatgaag ataagtaagt
      961 tctttttcgt tcccgcctga caactgccag cggcgggtat tatcaggtaa gttatcacca
     1021 cggcaatttg tcttacaccg gaaaggtgca atatttaccg caatgctacc cgttaatcct
     1081 ctggttttat ctttaggggt cagtaaccag tcgccagcta atgatgattg ttcaggttgg
     1141 gcgcatgcag tcagcagtaa tgtggcggta attagtgaca ctatgacgac cagactcctg
     1201 ttggtcttca ttgggatttc cttgagctac tcagtttgtt gacaaagtgt tcgttgtttt
     1261 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat cgcttaaatt caatatattg
     1321 caccatcctc gtaggcctga taagcgtagc gcatcaggca attctgtgtt tgtcatacgt
     1381 aaaaaagggc gatcttgcga ccgccctttt tttattaaat gtgtttacgc gtttttcaga
     1441 acttcgctaa caatctcaac cgcttctttc tcaatctgct tgcgatgttc ttcaccgagg
     1501 aagctttcgc agtagatctt atatgcgtct tccgtgcctg acggacgcgc ggcgaaccag
     1561 ccgttgtcag tcatcacttt cagaccgcca atagaagcac cgttgcccgg agcagcagtc
     1621 aggcgcgcgg tgatcgggtc acctgccagg gtgctggcgc tcaccatttc cggagacagc
     1681 ttagacagcg ccgctttttg tgcggaagtc gcagctgcct gcaaacggtt gtagctcggc
     1741 gcaccaaagc gttttgccag ttcgttgtag tgttcctgcg ggttcttacc ggtgacagcg
     1801 gtgatttccg ccgccagcag acacatgatg atgccgtctt tgtcggtgga ccacggcgtg
     1861 ccgtcgaaac gcaggaagga agcccctgcg ctctcttcgc cgccaaagcc gaagctgccg
     1921 tcgaacagac catcaacaaa ccatttgaaa cctaccggga cttctaccag cttacgaccc
     1981 aagtcattga ccacacggtc gatcatcgca gaagaaacca gcgttttacc aacggcaaca
     2041 tctttgcccc actgcggacg atgctggaac aggtaattga tcgccaccgc caggtagtgg
     2101 ttcggattca tcaaacctgc cggagtgacg ataccgtgac ggtcataatc cgggtcgtta
     2161 gcaaacgcca gatcgaactt atcacgcagt gccagcaggc ccgccatcgc acactcggag
     2221 gagcagtcca tacggatcgc gccgtcttta tcaaggtgca taaagcggaa ggtttgatcg
     2281 acctgatcgt taacgatagt caggttgagg ttgtaatact cgccaatgcg cttccagtat
     2341 tcgataccgg aaccgcccag cggatcaacg ccaagcgtca ggaccgcttt ctgaatcgcc
     2401 gccatatcaa cgatatcggc cagcccttcc acgaacggct gcaccagatc ctgctctttc
     2461 acatgaccgg atgccatcgc ttcgtcgagg gagatacgct tcacgccttt caggccatcg
     2521 gccagcagtg cgttggcacg atcttccacc actttagtga cgttggtatc agccgggcca
     2581 ccatttggcg gattgtactt gataccacca tcttccggcg ggttatggga cggtgtaata
     2641 acgataccgt ctgccagcgg gccacctttt ttattgtgaa ccaggatggc attggaaacg
     2701 gcaggcgtcg gggtgaagcc attgttttcc tgcacaataa catctacgcc gttcgctgcc
     2761 agcacttcca gaacggaaat aaatgcaggt tcggacaggg cgtgagtatc tttacccaca
     2821 tagcaagggc cagtgatacc gtttttcgca cgttcttcag caattgcctg agcgatcgcc
     2881 agaatgtgcg gctcgttaaa gctgtggcgc gctgcactgc cacggtgacc ggaagtaccg
     2941 aatttcaccg cgtgctccgc attccctgct tctggtttca gtacataata ttgcgccgtc
     3001 agttgggcga cgttaatcaa atcactctgt tgtgcaggtt ggcctgcacg attgtggatt
     3061 gccattgctt tgtcctttgt ctgcaacgtt ttagatagtt ccgcaaacct tctcaatcaa
     3121 ttccgccggg aattgcatcg actgcatgat gtgttcgatc atgctgcatt tacggccggt
     3181 gttggtgttg gtgatcaccc aatacggcgt gcctggcaca tgtttcggct tggtctgatt
     3241 accatttttc agcagcgttt gttcatctgc cgcaaagtaa acgcgtgtac gaccgtgcaa
     3301 cgattccgtt gcttcggcaa acgcctgggc gtcaagagaa tatagtgtag acaacagcag
     3361 cataaagcga ttgaccgctc gcttttgctc tgcgtattcg tccgaaagca gaagttcacg
     3421 cattgcgcga accttgtctt taatcgtttt gaccggcttc gcttcgacga tagcaggtga
     3481 cgcaacgcga acctctttcg tcaccggagc agcaggctgt gatgcggcgg aaaatttcaa
     3541 catacgccgt aaaatgtcgg atgcgctctc gccgatatgc ttagtgtggc tggcaatata
     3601 gctgtagagt tcatcatcaa cttcaatcgt tttcatctta atccagtgca gtgtcttagc
     3661 tgaatacaag tcgccaggag tatatgaata aatcccggca gcggatagcg tcaagcctgg
     3721 cttgcagaaa aactcggaat aaaccttatt tgttgcagcc ctgtggcaca atggtcaaca
     3781 taatacccta acccgacaac gcgaaaaaaa gaactttgcc atgaaattga atatccgcgc
     3841 gcaaactgca caaaaccagc acaataattc tcccatcgtt cttgtccatg gtctgtttgg
     3901 tagcctcgac aaccttggcg tactggctcg cgatctggta aacgatcaca atatcatcca
     3961 ggttgatatg cgtaaccacg gtctttcacc gagagagccg gtaatgaatt acccggcgat
     4021 ggcgcaggat cttgttgata ccctggatgc actgcagatc gacaaagcaa catttatcgg
     4081 tcactccatg ggcggtaaag cggtaatggc acttactgca ctggcccccg atcgcatcga
     4141 taaactggtg gcgatcgata tcgcaccggt cgactatcac gtacgccgtc atgatgagat
     4201 tttcgcagct atcaacgcgg tcagtgaatc ggacgcacaa actcgccagc aagcagcagc
     4261 aataatgcgc cagcatctta atgaagaagg ggtgattcag tttctgttga aatcttttgt
     4321 tgacggggag tggcgcttta acgtgccggt attgtgggat cagtatccgc atattgtggg
     4381 ttgggagaaa atcccggcat gggatcaccc tgccctgttt atccctggtg gtaattctcc
     4441 gtatgttagc gagcagtacc gtgatgattt actggcacaa tttccacagg cacgggcgca
     4501 tgtcattgca ggcgcgggtc actgggtcca tgctgaaaaa ccagatgcgg tattacgcgc
     4561 tatccgtcgc tatctcaatg attaacgcaa ctgattaaaa acccagcgtc cgcgtcgctt
     4621 tccgcgcgtg ggcgttggcg tgactcatcg cgctgatgta ttatggcgcg ctatccatcc
     4681 gggcagaggc ctggctgatt gtttcccccg aagtcaccaa gatcatggcc aaagaacaaa
     4741 cggaccgtac gacattagat ctgttcgcgc acgagcgtcg accgggacga ccgaaaacta
     4801 atccgctttc gcgcgatgaa cagctgcgta ttaataaacg caaccaacta aaacgcgaca
     4861 aagtacgtgg ccttaagcgt gtcgaactga agctgaacgc ggaagctgtc gaggcgctga
     4921 acgagctggc ggaatcgcgc aatatgagcc gtagcgaact gatcgaagag atgttaatgc
     4981 agcaactggc ggcgctgcgt agtcagggca tcgtttaaat ttccactttc atgtagcaca
     5041 gtgtgcagtc ctgctcgttt gctgattccg caagactgcc tgttctgcta tgattgcctt
     5101 tatccgtggg caattttcca cccccatttc aataagtttc aagaggttat ttcactcatg
     5161 gctatcactg gcatcttttt cggcagcgac accggtaata ccgaaaatat cgcaaaaatg
     5221 attcaaaaac agcttggtaa agacgttgcc gatgtccatg acattgcaaa aagcagcaaa
     5281 gaagatctgg aagcttatga cattctgctg ctgggcatcc caacctggta ttacggcgaa
     5341 gcgcagtgtg actgggatga cttcttcccg actctcgaag agattgattt caatggcaaa
     5401 ctggttgcgc tgtttggttg tggtgaccag gaagattacg ccgaatattt ctgcgacgca
     5461 ttgggcacca tccgcgacat cattgaaccg cgcggtgcaa ccatcgttgg tcactggcca
     5521 actgcgggct atcatttcga agcatcaaaa ggtctggcag atgacgacca ctttgtcggt
     5581 ctggctatcg acgaagaccg tcagccggaa ctgaccgctg aacgtgtaga aaaatgggtt
     5641 aaacagattt ctgaagagtt gcatctcgac gaaattctca atgcctgatg tgatgcggcg
     5701 tagactcatg tctacgccgc attaatagat aatgccaatc aaaataattg ctacaaattt
     5761 gtaacttttg ctgttgtacc tgtacaatgt cccggtgttc aagtggcctt gccgttgtaa
     5821 atgtaagcta tgccacgttt ttattaacaa tatttgccag ggacttgtgg ttttcattta
     5881 ggcgtggcaa ttctataatg atacgcatta tctcaagagc aaattctgtc acttcttcta
     5941 atgaagtgaa ccgcttagta acaggacaga ttccgcatga ctgataacaa taccgcccta
     6001 aagaaagctg gcctgaaagt aacgcttcct cgtttaaaaa tcctggaagt tcttcaggag
     6061 ccggacaacc atcacgtcag tgcggaagat ttatacaaac gtctgatcga tatgggtgaa
     6121 gaaattggtc tggctacggt atatcgcgta ctgaaccagt ttgacgacgc tggtatcgtc
     6181 acccgccaca attttgaagg cggtaaatcc gtatttgaac tgacacagca acatcaccac
     6241 gatcacctga tctgcctcga ctgcggcaag gttatcgaat ttagtgatga ttccatcgaa
     6301 gcgcgtcagc gtgaaattgc cgcaaaacat ggcattcgcc tgactaacca cagtctctat
     6361 ctttacggtc actgtgccga aggcgattgc cgcgaagatg agcacgcgca cgaaggcaaa
     6421 taagccagcc tgaacgagaa aagccaacct gcgggttggc ttttttatgc aagggaaata
     6481 agaaaagcga gagttacagc tctcacttat ttgttattac tgcgaatttc ctgccagacc
     6541 ttatcacaat cggctttcac cgcctgatca tttccggttt gcgttgcacg cagacactgc
     6601 tgataatcca gtacgcggac actttcctgg tcggcaaact gctgatgttg tttctctttc
     6661 ttcagcacgt ttaacacgct ctggcaggct tcaatttttt ccggcgaacc ttgcgcggtg
     6721 ttgatacagg cgctgtatgc ctctttcaga cggctgtctt cctgtggtgc ggttgattgc
     6781 gcacaagcta ccagccccga tgccatcatg gcgatgagaa tcagtttttt catagcaaat
     6841 tcctcaaacg tgcggcaggc acaaaacctg ccgcgtcggg catcagaaga tggtgaatgg
     6901 tgcgattacc ataaatttta cgtcacgctc atcctggaag atgttgccgt aaccaccgcc
     6961 ccagcttggg atgtcggagt ggttgtcgta ttcggtgaag tgcagtttga acatcgtgcc
     7021 tttggcgcga ccgtcctgaa tggtatagac cgcatccagg ctgtatgcag actcttcaat
     7081 agtccggttt ttgtcgtagt acgcatccgg attgctctgc taggtcgcag gtttagcatc
     7141 ccatgcgtaa acgtaggaag cgccgatggc gaagcctgga agattccagt ttttcaggtc
     7201 atacatcgca ccgaagaaga ctgctttttc gccgttggcg ttgaagtcag aacggttgtc
     7261 ccaccagata tccaggcgac cgtttgagga agcgtaggtt ggagtcatac gttgcaggaa
     7321 gtatccctgc tgaccgtcag ccttaaccca ggtgccttcg aggcgcaaat ccactacgtc
     7381 agccgcccgg taaccaaagg tcaacgcctg cagccaggcg gtgccgtcat aaaggtcgtt
     7441 gacgctgcga tcgtcaactt tatcgcgggt accgtagaac tggtagctgg tggttaacgg
     7501 gctaccggcg atatcaaatt tgtagctggc tttggcaaaa tattgatcga tatacccttc
     7561 cgcctgacca aaagccgctt ccagtacgaa gttatttttg aagtcgtatt tcgccccaag
     7621 ggagtgcaga taatcaactt tggtggtttt atcgttctga taaaactcat ccatttccag
     7681 atgccacggc gctttgtatt cgttggtcca catgtaggag aaactcaacg caccggcatc
     7741 gccgtaatca aaattcgctc cggcttccgc accctgataa gtacccggca taaagctcca
     7801 gtgaggcgct aacagcgtct gaccggttgg ctgaatataa cctgccctcg cccaaaccgg
     7861 accgtattta aatttggccg ctgctttata caggcttata ccgcttttgt cgccggacca
     7921 gtcttcgtca taggctttat tactttttga aaacgcgatt tcgttcgggt gagagctgtc
     7981 gccgttttcc gccatttcaa tcgccgtaaa cgcggcaata tcaaggccga acatatcagc
     8041 agcataacca gactgaaaat cgaggttggc attccaggtg gagtgagaaa ggttggtttt
     8101 gtatttgtcg ccgtcggtaa catctttacg gtcgcgttca cgctgccagt aatatatacc
     8161 gccggttaag gttgaatcgt cgatgaatcc ttcggcgcga gcctccggca ttgccataaa
     8221 gcccgacatt gctgtaacac cggcgatagc cagcgccagc gtactacgtt tgccactaaa
     8281 cgtacgcatg ggttaatcct ctttgacgta taaattgctg caccaaaggt gaacagcgaa
     8341 aaaataaaaa ttaaaaaagt agcgttgaga aaagtctcgc tgactacagg aacagactat
     8401 ttttcgcgac gcgaattatc aatctctttc tgtaaccaga atgtaagaac atgagcaacc
     8461 gcacatattt tgatgatttt tgttacaagg cttaagtttg cggttgaaag gcttgcagaa
     8521 gaataaaagc gtgaaattcc agcaagatga aagcggttac attttattta attatgtttt
     8581 gttattttaa atttatttta tggataaatt gcgaagcgga attaattcgc gaa
//
LOCUS       DAPHBJ010000180         8608 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.182, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000180 DAPHBJ010000000
VERSION     DAPHBJ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.182"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..604)
                     /gene="upp"
                     /locus_tag="RHZ76_003698"
     CDS             complement(<1..604)
                     /gene="upp"
                     /locus_tag="RHZ76_003698"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDV2814267.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAG"
     gene            929..1966
                     /gene="purM"
                     /locus_tag="RHZ76_003699"
     CDS             929..1966
                     /gene="purM"
                     /locus_tag="RHZ76_003699"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDV2814268.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            1966..2604
                     /gene="purN"
                     /locus_tag="RHZ76_003700"
     CDS             1966..2604
                     /gene="purN"
                     /locus_tag="RHZ76_003700"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDV2814269.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDMVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYATDE"
     gene            2775..4841
                     /gene="ppk1"
                     /locus_tag="RHZ76_003701"
     CDS             2775..4841
                     /gene="ppk1"
                     /locus_tag="RHZ76_003701"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HDV2814270.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            4846..6387
                     /gene="ppx"
                     /locus_tag="RHZ76_003702"
     CDS             4846..6387
                     /gene="ppx"
                     /locus_tag="RHZ76_003702"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HDV2814271.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(6426..>8608)
                     /gene="pdeF"
                     /locus_tag="RHZ76_003703"
     CDS             complement(6426..>8608)
                     /gene="pdeF"
                     /locus_tag="RHZ76_003703"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HDV2814272.1"
                     /translation="PSLILPIFAHINTFAHISSGEVFLFYLPLALMISMMMFFSWAAL
                     PGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGGYRVFTPRRNNVSHGDTRLISQ
                     RIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMPFNLGTLINYQALLVGNLIGVP
                     LCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFALWLLALGALLLLLCMPLNEKST
                     IFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMISIHSYQNYIPIYPGYTTQLTI
                     TSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDPVVHLPNVRALNRALRDAPWSA
                     LCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTES
                     HQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIV
                     TNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILL
                     RMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSP
                     TSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAI
                     DDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAE
                     YVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT         2217 a         2125 c         2156 g         2110 t
ORIGIN      
        1 caccggcatc gccgaggccc ggaataatgt atccgtgctc gttcagtccc tgatcaatcg
       61 atgcggtata cagttcgacg tccgggtgcg ctttttccag cgcagcgata ccttctggcg
      121 cagcgaccag caccagaact ttgatgctgc tgcagcccgc ttttttcagc aggtcgatgg
      181 tcgcgataac ggaaccaccg gttgccagca ttgggtcaac gatcagcgcc atacgctcat
      241 cgatgttaga aaccagtttc tggaagtacg gtaccggctc cagcgtttct tcattacggt
      301 acataccgac aacgctgatg cgcgcgctcg gaacgttttc cagcacaccg tccatcatac
      361 caagacccgc acgcagaatt ggcacaacgg taattttctt acctttgatc tggtcgattt
      421 ctaccgggcc gttccagcct tcgatagtta ctttttccgt ttcgaggtcg gcggtcgctt
      481 cgtaagtcag caggctaccc acttcggaag cgagttcgcg aaagcgcttg gtgctgatat
      541 cttgctcacg catcagtccc agcttgtgtt tgacgagtgg gtgtttgact tccacgatct
      601 tcatactctt tctcctttga ggggcagcca caaaaaaaat cgacggatta tacctccttt
      661 cttcaaggcg gcaatattat tttcgttgac tttagtcaaa atgataacag tttgagataa
      721 agttatttta tattcaaatg gttatgaaag aagattattc catccgaaaa ctaacctttg
      781 ccctggcaca agtcttcttt cgccgcgcgc ctgggcaaaa gacgtgcaaa aaggttgtgt
      841 aaagcagtct cgcaaacgtt tgctttccct gttagaattg cgccgaattt tatttttcta
      901 ccgcaagtaa cgcgtgggga cccaagcagt gaccgataaa acctctctta gctacaaaga
      961 tgccggtgtt gatattgacg cgggtaatgc tctggttgga agaatcaaag gcgtagtgaa
     1021 gaaaacgcgt cgtccggaag tgatgggcgg tctgggcggc ttcggtgcgc tgtgtgcatt
     1081 gccgcaaaaa tatcgtgaac ccgtgctggt ttccggcact gacggcgtag gtaccaagct
     1141 gcgtctggca atggacttaa aacgtcacga caccattggt attgatctgg tcgccatgtg
     1201 cgttaatgac ctggtggtgc aaggtgcaga gccgctgttt ttcctcgact attacgcaac
     1261 cggaaaactg gatgttgata ccgcttcagc ggtgatcagc ggtatcgcgg aaggatgtct
     1321 gcaatcgggc tgttcactgg tgggtggcga aacggcagaa atgccgggga tgtatcacgg
     1381 tgaggattac gatgtcgcgg gtttctgcgt tggcgtggta gaaaaatcag aaatcatcga
     1441 cggctctaaa gtcagcgacg gcgatgtgct gattgcactc ggttccagcg gtccgcactc
     1501 gaacggctat tcgctggtgc gcaaaattct tgaagtcagc ggttgtgatc cgcaaaccac
     1561 cgaacttgat ggtaagccat tagccgatca tctgctggca ccgacccgca tttacgtgaa
     1621 gtcagtgctg gagttgattg aaaaggtcga tgtgcatgcc attgcgcacc tgaccggcgg
     1681 cggcttctgg gaaaacattc cacgcgtatt gccagataat actcaggcag tgattgatga
     1741 atcttcctgg cagtggccgg aagtgttcaa ctggctgcaa acggcaggta acgttgagcg
     1801 ccatgaaatg tatcgcacct tcaactgcgg cgtcgggatg attatcgccc tgcctgctcc
     1861 ggaagtggac aaagccctcg ccctgctaaa cgccaacggt gaaaacgcgt ggaaaatcgg
     1921 tatcatcaaa gcctctgatt ccgaacaacg cgtggttatc gaataatgaa tattgtggtg
     1981 cttatttccg gcaacggaag taatttacag gcaattattg acgcctgtaa aactaacaaa
     2041 attaaaggca ccgtacgggc agttttcagc aataaggccg acgcgttcgg ccttgaacgc
     2101 gcccgccagg cgggtattgc aacgcatacg ctcatcgcca gcgcgtttga cagtcgtgaa
     2161 gcctatgacc gggagttgat tcatgaaatc gacatgtacg cacccgatat ggtcgtgctg
     2221 gctggtttta tgcgcattct cagcccggcg tttgtctccc actatgccgg gcgtttgctg
     2281 aacattcacc cttctctgct gccgaaatat cccggattac acacccatcg tcaggcgctg
     2341 gaaaatggtg atgaagagca cggtacatcg gtgcatttcg tcaccgatga actggacggt
     2401 ggcccggtta ttttacaggc gaaagtcccg gtatttgctg gtgatacgga agatgacatc
     2461 accgcccgcg tgcaaaccca ggaacacgct atttatccac tggtgattag ctggtttgcc
     2521 gatggtcgtc tgaaaatgca cgaaaacgcc gcgtggctgg atggtcaacg tctgccgccg
     2581 cagggctacg ctaccgacga gtaatgcccc gtagttaaag cgccagctct gccgctggcg
     2641 tttttcaatt cacctgtaaa tcgcaagctc cagcagtttt tttccccctt ttctggcata
     2701 gttggacatc tgccaatatt gctcgccata atatccaggc agtgtcccgt gaataaaacg
     2761 gagtaaaagt ggtaatgggt caggaaaagc tatacatcga aaaagagctc agttggttat
     2821 cgttcaatga acgcgtgctt caggaagcgg cggacaaatc taacccgctg attgaaagga
     2881 tgcgtttcct ggggatctat tccaataacc ttgatgagtt ctataaagtc cgcttcgctg
     2941 aactgaagcg acgcatcatt attagcgaag aacaaggctc caactctcat tcccgccatt
     3001 tactgggcaa aattcagtcc cgggtgctga aagccgatca ggaattcgac ggcctctaca
     3061 acgagctatt gctggagatg gcgcgcaacc agatcttcct aattaatgaa cgccagctct
     3121 ccgtcaatca acaaaactgg ctgcgtcatt attttaagca gtatctgcgt cagcacatta
     3181 cgccgatttt aatcaatcct gacactgact tagtgcagtt cctgaaagat gattacacct
     3241 atttggcggt ggaaattatc cgtggcgata ccatccgtta cgcgctgctg gagatcccat
     3301 cagataaagt gccgcgcttt gtgaatttac cgccagaagc gccgcgtcga cgcaagccga
     3361 tgattcttct ggataacatt ctgcgttact gccttgatga tattttcaaa ggcttctttg
     3421 attatgacgc gctgaatgcc tattcaatga agatgacccg cgatgccgaa tacgatttag
     3481 tgcatgagat ggaagccagc ctgatggagt tgatgtcttc cagtctcaag cagcgtttaa
     3541 ctgctgagcc ggtgcgtttt gtttatcagc gcgatatgcc caatgcgctg gttgaagtgt
     3601 tacgcgaaaa actgactatt tcccgctacg actccatcgt ccccggcggt cgttatcata
     3661 attttaaaga ctttattaat ttccccaatg tcggcaaagc caatctggtg aacaaaccac
     3721 tgccgcgttt acgccacatt tggtttgata aagcccagtt ccgcaatggt tttgatgcca
     3781 ttcgcgaacg cgatgtgttg ctctattatc cttatcacac ctttgagcat gtgctggaac
     3841 tgctgcgtca ggcctcgttc gatccgagcg tgctggcgat taaaatcaac atttaccgtg
     3901 tggcaaaaga ttcacgtatc atcgactcga tgatccacgc tgcgcacaac ggcaagaaag
     3961 tgaccgtggt ggttgagcta caggcacgtt ttgacgaaga agccaacatt cactgggcga
     4021 agcgcctgac cgaagcaggc gtgcacgtta tcttctctgc gccggggctg aaaattcacg
     4081 ccaaactgtt cctgatttca cgtaaagaaa acggtgaagt ggtgcgttat gcacacatcg
     4141 ggaccgggaa ctttaacgaa aaaaccgcgc gtctttatac tgactattcg ttgctgaccg
     4201 cagatgcgcg tatcaccaac gaagtacggc gggtatttaa ctttattgaa aacccatacc
     4261 gcccagtgac atttgattat ttaatggtgt caccgcaaaa ctctcgccgt ctgttatatg
     4321 aaatggtaga ccgcgaaatc gccaacgcgc agcaagggct gcccagtggt atcaccctga
     4381 agctaaataa ccttgtcgat aaaggcctgg ttgatcgtct gtatgcggcc tccagctccg
     4441 gcgtaccggt taatctgctg gttcgcggaa tgtgttcgct gatccccaat ctggaaggca
     4501 ttagcgacaa cattcgtgcc atcagtattg ttgaccgtta ccttgaacat gaccgggttt
     4561 atatttttga aaatggcggc gataaaaagg tctacctttc ttccgccgac tggatgacgc
     4621 gcaatattga ttatcgtatt gaagtggcga caccgctgct cgatccgcgc ctgaagcagc
     4681 gggtgctgga catcatcgac atattgttca gcgatacggt caaagcacgt tatatcgata
     4741 aagaactcag taatcgctac gttccccgcg gcaatcgccg caaagtacgg gcgcagttgg
     4801 cgatttacga ctacatcaaa tcactcgaac aacctgaata accctatgcc aatacacgat
     4861 aaatcccctc gtccgcagga gtttgctgcg gtcgatcttg gttcaaacag ttttcacatg
     4921 gtcatagccc gtgtggtaga tggtgccatg cagattattg gccgcctgaa acagcgggtg
     4981 catctggcgg acggcctggg gccagataat atgttgagtg aagaggcaat gacgcgcggt
     5041 ttgaactgtc tgtcgctgtt tgccgaacgg ctacaagggt tttctcctgc cagcgtctgt
     5101 atagtcggca cccatacgct gcgtcaggcg ctgaacgcca ctgactttct gaaacgcgcg
     5161 gaaaaggtca ttccctaccc gattgaaatt atttccggta atgaagaagc ccgtctgatt
     5221 tttatgggcg tggaacatac ccaaccggag aaaggtcgca aactggttat tgatattggc
     5281 ggcggatcta cggaactggt gattggtgaa aatttcgaac cgattctcgt tgaaagccgc
     5341 cggatgggtt gtgtcagctt tgcccagctt tacttccctg gcggggtcat caataaagag
     5401 aattttcagc gcgctcgtat ggcggcagca caaaaactgg aaactttaac ctggcaattc
     5461 cgtattcagg gctggaacgt ggcaatgggc gcttccggta ccataaaagc cgcccatgaa
     5521 gtgttaatgg aaatgggcga gaaagacggg ataattaccc cggaacgtct ggaaaaactg
     5581 gtaaaagaag ttttacgtca ccgtaatttc gcatcgctga gtttaccggg tctttccgaa
     5641 gagcggaaaa cagtcttcgt tcctggactg gcgattttat gcggtgtgtt tgatgcttta
     5701 gccatccgtg aactgcgcct ttctgacggg gcgttacgcg aaggcgtact gtatgaaatg
     5761 gaaggacgtt tccgtcatca ggatgtgcgt agtcgcaccg ccagcagcct cgccaaccag
     5821 tatcacatcg acagcgagca ggcccgacgg gtgctggata ccactatgca aatgtacgaa
     5881 cagtggcggg aacagcaacc gaagctggcg catccgcaac tggaggcgct actgcgatgg
     5941 gccgccatgc tgcatgaggt cgggttgaat atcaaccaca gcggtttgca tcgccactcc
     6001 gcttatattc tgcaaaacag tgacttgccg ggttttaatc aggaacagca gctgatgatg
     6061 gcgacactgg tgcgctatca ccgtaaagcg attaagctcg acgatctgcc gcgctttacc
     6121 ttgtttaaga agaaacagtt cctgccactg atacagctat tgcgccttgg cgtattacta
     6181 aacaatcaac gtcaggcaac caccacaccg ccaacattga cactgattac tgatgacagt
     6241 cactggacac tgcgtttccc gcatgactgg tttagtcaga atgcgctggt actgcttgat
     6301 ctggaaaagg agcaagaata ctgggaaggc gtggctggct ggcggttgaa aattgaagaa
     6361 gaaagtacac ctgaaatcgc cgcttaatgc tttgcgggcc gacgagaaat gtcggcccgc
     6421 attattcagg cactttcgcg aatgggttcg atttcattca gcgtatcaat taacggctgc
     6481 ggcttaccaa taagataacc ctgcatataa tcgatcccca aagagagcac cgcctcgcgg
     6541 atctcttcgt tttcaacgta ctctgccact acccgcattt tcttcattcg cgccaggtgg
     6601 caaatcgacg ccactatctg ataatccaga ctatttgaca caatattgcg gataaagctg
     6661 ccgtcaattt taagcagatc ggcattcaca tttttaagcc gtgcatagct ggcgtagccg
     6721 gtgccgaaat catcaatcgc aatctggcag cctaattcct gaagatgctg caaggtgatt
     6781 tgcgcctgct taacattggt cagagcatta ctttcggtga cttcaaaaat aagttgccac
     6841 gcttcaatct gatatttagc cagcaactga ctgacttcaa caggaaaacg agcctgacat
     6901 accgaggttg gagacagatt aatagcaaaa cggtgagcgg gcatcttcgc tctgttttca
     6961 gccataaatt gcagcgtatg ctcaatgacc cacatgtcga tactcgacga taaaccaaat
     7021 tcgtgcgcga ccggcaagaa gctatcgggg ctgatcagtt catcattctc acctttcatg
     7081 cgcagaagaa tttcatggta aacatcacca cgcataccgg taatcggctg ggccatcagg
     7141 aaaaaatggt tgtgttccag cgcctgctgt agtcgattca tcatcgcgac tttatctttc
     7201 aattcgcgtt gcaaatacat tgccccgcga cgctgcatat tttccggggc gttggtcacg
     7261 atggaaagtt cggcgaccgt atttagctct cccagcagca ggtagatatg attcactggc
     7321 gagcgcacat agcagtaact gacgccaatc tgcggttgca tcggcatgcc atcccaaaag
     7381 aaacgaaatt gcttgagatg gctatccagt gcggtaatgc gctcctggtg cgattctgta
     7441 ttcagtcgca gcgcgagatc gttacccgaa agctgataaa catcttcacc cggttccagc
     7501 aagggtgaca gccagtgaga aagtttttgc ttgtattgaa tccgcagcat gatgccatag
     7561 ttcttaacca gcatttccat gccagggatg cgtaaataac aaagcgcaga ccagggggca
     7621 tcacgcaacg cgcgattcag ggcgcgaaca tttggcagat gaaccaccgg gtccacatac
     7681 gcaagccgct gaatgcgtct gactaccgct ctctgacggg ttgccagtac agccatataa
     7741 ttgacaataa aagagaatac cagataactg gaggaggtta tggtcagctg cgtggtatag
     7801 ccaggataaa tgggaatgta attttgatag ctgtggatgc tgatcatcaa cacgaccgcc
     7861 cagagcagcg aaatcagctt ataaccatag cgcatcgctc cccacatcat caggggcagc
     7921 aataatgaca aggtataatt ggtgctaaaa attgtgcttt tttcatttaa cggcatgcat
     7981 aacagcaaca ataaagcacc taatgccagt agccagagcg cgaactcttt tttggtgact
     8041 ttggcatcaa cctgctgttt taattgcgaa taatagctac gtaaataaaa tggatttcgc
     8101 actacccgaa tgatgaagta gcacagcggg acaccgatca gattacccac cagtaaggcc
     8161 tgataattga ttaaagtccc gaggttaaaa ggcatgacac cgaccagatt ttctctgctc
     8221 gccagtaatc ctacaaatgc agcaaactgg aaaagtatca gaaacagcgt tgcaggaaac
     8281 acaatctgcc agaatatacg ctgggaaatt aaacgggtat caccatgtga aacgttgtta
     8341 cgccggggag taaagaccct gtaaccgccc caacagagaa tgataatgat aataaaatta
     8401 gccgttaatg atagcgtttc gtaaaaaccc agctctgcat atttgcggac aaaaatccct
     8461 aaggcgatcc ctggcaatgc cgcccagctg aaaaacatca tcatgctgat catcagtgcc
     8521 agaggcagat aaaagagaaa aacctcaccg gaagaaatat gcgcgaaagt attaatgtgg
     8581 gcgaaaatgg gcaagattaa agaaggca
//
LOCUS       DAPHBJ010000181         8594 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.183, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000181 DAPHBJ010000000
VERSION     DAPHBJ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.183"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            350..7426
                     /gene="yeeJ"
                     /locus_tag="RHZ76_003704"
     CDS             350..7426
                     /gene="yeeJ"
                     /locus_tag="RHZ76_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
                     /protein_id="HDV2814273.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLVTQLVFPMAAA
                     AQGVVNAAIQQPVPAQIAIANTNTVPYTLGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSEKKLTPPPGNSSDNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAEGWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVAARRSLAGSRYDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNFSNREQSMVVVQAPTLSQKDSSVSLSTQTLNADSHSTATLTFIAHDAAGNPV
                     IGLVLSTRHEGVQDITLSDWKDNGDGSYTQVLTTGALSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNTAVSIDNV
                     KPGVTTDWKETTDGVYKATYTAYTKGSGLTAKLLMQSWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATIDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVKVTFNVNSAEAKLSQTEVNSHDGIATATLTSLKNGDYT
                     VTASVSSGSQANQQVNFIGDQSTAALTLSVPSGDITVTNTAPQYMTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNGGKGMTDSNGVAIATLTGTLAGTHMITARLANSNVSDA
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPSNHPVAGITVTFTMPQDV
                     AANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLGNNNTSDSQPVTFVADKTSA
                     QVVLQMSKDEITGNGVDNATLTATVKDQFDNEVNNLPVTFSSASSGLTLTPGVSNTNE
                     SGIAQATLAGVAFGEQTVTASLANNGASDNKTVHFIGDTAAAKIIELTPVPDSIIAGT
                     PQNSSGSVITATVVDNNGFPVKGVTVNFTSRTNSAEMTNGGQAVTNEQGKATVTYTNT
                     RSSIESGARPDTVEASLENGSSTLSTSINVNADASTAHLTLLQALFDTVSAGDTTNLY
                     IEVKDNYGNGVPQQEVTLRVSPSEGVTPSNNAIYTTNHDGNFYASFTATKAGVYQVTA
                     TLENGDSMQQTVTYVPNVANAEITLAASKDPVIADNNDLTTLTATVADTEGNAIANTE
                     VTFTLPEDVKANFTLSDGGKAITDAEGKAKVTLKGTKAGAHTVTASMTGGKSEQLVVN
                     FIADTLSAQVNLNVTEDNFIANNVGMTTLQATVTDGNGNPLANEAVTFTLPADVSASF
                     TLGQGGSAITDINGKAEVTLSGTKSGTYPVTVSVNNYGVSDTKQVTLIADAGTAKLAS
                     LTSVYSFVVSTTEGATMTASVTDANGNPVEGIKVNFRGTSVTLSSTSVETDDRGFAEI
                     LVTSTEVGLKTVSASLADKPTEVISRLLNASADVNSATITSLEIPEGQVMVAQDVAVK
                     AHVNDQFGNPVAHQPVTFSAEPSSQMIISQNTVSTNTQGVAEVTMTPERNGSYMVKAS
                     LPNGASLEKQLEAIDEKLTLTASSPLIGVYAPTGATLTATLTSANGTPVEGQVINFSV
                     TPEGATLSGGKVRTNSSGQAPVVLTSNKVGTYTVTASFHNGVTIQTQTTVKVTGNSST
                     AHVASFIADPSTIAATNTDLSTLKATVEDGSGNLIEGLTVYFALKSGSATLTSLTAVT
                     DQNGIATTSVKGAMTGSVTVSAVTTAGGMQTVDITLVAGPADTSQSVLKSNRSSLKGD
                     YTDSAELRLVLHDISGNPIKVSEGMEFVQSGTNVPYIKISAIDYSLNINGDYKATVTG
                     GGEGIATLIPVLNGVHQAGLSTTIQFTRAEDKIMSGTVSVNGTDLPTTTFPSQGFTGA
                     YYQLNNDNFAPGKTAADYEFSSSASWVDVDATGKVTFKNVGSYSERITATPKSGGPSY
                     VYEIRVKSWWVNAGEAFMIYSLAENFCSSNGYTLPRANYLNHCSSRGIGSLYSECGDM
                     GHYTTDAGFQSNMYWSSSPANSSEQYVVSLATGDQSVFEKLGFAYATCYKNL"
     gene            complement(7688..>8594)
                     /locus_tag="RHZ76_003705"
     CDS             complement(7688..>8594)
                     /locus_tag="RHZ76_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDV2814274.1"
                     /translation="ALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHAT
                     LKEIKDLAKNITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCFSPL
                     CKNASRMDQKLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYI
                     AFNLEGSSQERTFSLSIAENLIAKIQRETDIPIVIVHGPKGEDKARALVDCYNNVYRL
                     SLPPSIKRSAAIIKDAYIAITPGTSILHMASAYNTPVVAIYADYKTRWPAMADVSESV
                     VVGQKIDNISLDEFAKALKSVLARI"
BASE COUNT         2421 a         2102 c         2053 g         2018 t
ORIGIN      
        1 atccgtatgt cactggttcg agtccagtca gaggagccaa attctaaaaa ttcgcttctt
       61 agcgcaatgt cactgacctt aattgagcat tgtttttcaa cagatagcgg gttttgacat
      121 cttaagcgcc ctcgaccttt atggttgagg gcgttttgct atggcgccat caccattttc
      181 ccctcgatta taaaacttga gtaattcagt agtctcccct cttgcaactc acacccaaaa
      241 ctgcctaacg aaaagttatt aattttcaat catattgcta tcaggattta cattttttcg
      301 ctgtgctaga aagggcgcat ttatgttagc tcgttcaggg aaggtaagca tggctacgaa
      361 gaagagaagt ggagaagaaa taaatgaccg acaaatctta tgcgggatgg gtatcaaact
      421 acgccgctta actgcgggta tctgcctggt aactcaactt gtgttcccta tggctgcggc
      481 agcacaaggt gtggtaaacg ccgcaatcca acaaccagtt cctgcacaaa tcgccattgc
      541 aaataccaat acggtgccct acaccctagg agcgctggaa tcggcccaaa gcgttgccga
      601 acgtttcggt atttcggtgg ctgagttacg caaactcaac caatttcgta cgtttgctcg
      661 aggttttgat aatgtccgcc agggtgatga actggatgtc ccggcacaag ttagtgaaaa
      721 aaaattaacc ccgccgccgg gtaatagcag tgacaacctc gagcaacaga tagccagtac
      781 ttcacagcaa atcgggtctc tgctcgccga ggatatgaac agcgagcaag cggcaaatat
      841 ggcgcgtgga tgggcctctt ctcaggcttc aggcgcaatg acagactggt taagccgctt
      901 cggtaccgca agaatcacgc tgggcgtgga tgaagatttt agcctgaaga actcccagtt
      961 cgattttctc catccgtggt atgaaacgcc tgataatctc tttttcagtc agcatactct
     1021 ccatcgtact gacgagcgta cgcagattaa caacggcttg ggttggcgtc atttcactcc
     1081 cacatggatg tcgggcatca acttcttttt cgaccacgat cttagccgtt accactcccg
     1141 cgccggcatt ggcgcggagt actggcgcga ctatcttaaa ttaagcagta acggctattt
     1201 gcgactgacc aactggcgca gcgcacctga actggacaac gattatgaag cacgcccggc
     1261 caatggctgg gatgtacgcg cagaaggctg gttacccgcc tggccgcacc ttggcggtaa
     1321 actggtctat gaacagtatt atggcgatga agtggccctg ttcgataaag acgatcggca
     1381 aagtaatcct catgccataa ccgctggact aaactatacc cccttcccgc tgatgacctt
     1441 tagcgcggag caacgccagg gtaaacaggg cgaaaatgac acccgatttg ccgtcgattt
     1501 tacctggcaa cctggcagcg caatgcagaa acagcttgac ccgaatgaag tcgctgcacg
     1561 gcgtagcctt gcaggcagcc gttatgatct ggtggatcgc aacaacaaca tcgttctgga
     1621 atatcgcaaa aaagaactgg tccgcctgac cctgacagac cccgtgacag ggaagtcagg
     1681 agaagtgaaa tcactggttt cgtcgctaca aaccaaatat gccctgaaag gctataacgt
     1741 cgaagccacc gctctggaag ctgccggtgg taaagtggtt acaacgggta aagatattct
     1801 ggttaccctg ccggcgtacc ggttcaccag tacgccagaa accgataaca cctggccgat
     1861 tgaagtcacc gctgaagatg tcaaaggcaa tttttcgaat cgtgaacaga gcatggtagt
     1921 cgttcaggca cctacgctaa gccagaaaga ttcctcagta tcattaagta cccaaacatt
     1981 gaacgcggat tcccattcaa ccgccacact gacttttatt gctcatgatg cagcaggtaa
     2041 tcctgttatc gggctggtgc tttcgacgcg tcacgaagga gttcaggaca tcaccctgtc
     2101 tgactggaaa gataacggtg acggaagcta tacccaagtc ctgaccacag gagcgctgtc
     2161 tggcacgctg acgcttatgc cacagctgaa cggtgtggat gcggctaaag cccccgccgt
     2221 ggtgaatatc atttctgttt cgtcatcccg gactcactcg tcaattaaga ttgataagga
     2281 ccgttatctc tccgggaatc ctatcgaggt aacggtagaa ctgagagatg aaaatgacaa
     2341 acctgttaag gagcaaaaac agcaactgaa taccgcagtc agcatcgaca acgtgaaacc
     2401 tggtgtcact acagactgga aagaaaccac agatggcgtc tataaggcaa cctataccgc
     2461 ctataccaaa ggcagtgggc ttactgcgaa gctattaatg caaagctgga atgaagattt
     2521 gcataccgct ggatttatca tcgacgccaa cccgcagtca gcgaaaattg cgacattatc
     2581 tgccagcaat aatggtgtgc tcgccaatga gaatgcagca aacaccgtct cggtcaatgt
     2641 cgctgatgaa ggaagcaacc caatcaatga tcataccgtc acgtttgcgg tattaagcgg
     2701 atcggcaact tcctttaaca atcaaaacac cgcaaaaacg gatgttaatg gtctggcgac
     2761 tattgatctg aaaagtagta agcaggaaga caacacggtt gaagtcaccc ttgaaaatgg
     2821 cgtgaaacaa acgttaatcg tcagttttgt cggcgactcg agtaccgcac aggttgatct
     2881 gcagaagtcg aaaaatgaag tggtcgctga cggcaatgac agcgccacaa tgaccgcgac
     2941 agttcgggat gcaaaaggca acctgctcaa tgacgtcaag gtcaccttca atgttaattc
     3001 agcagaggcg aaactgagcc aaaccgaagt gaatagccac gacgggatcg ccacagctac
     3061 gctgaccagt ttgaaaaacg gtgattatac ggttacggcc tctgtgagct ctggttccca
     3121 agccaatcaa caggtgaatt ttattggtga tcaaagtact gctgccctga ccctcagtgt
     3181 gccttcaggt gatatcaccg tcaccaacac agctccgcaa tatatgactg caaccttgca
     3241 ggataaaaat ggcaacccac taaaagataa agaaatcacc ttctctgtgc caaacgacgt
     3301 cgcaagtcgg ttctcgatta gcaacggcgg aaaaggcatg acggatagta acggggttgc
     3361 aatcgccacc ctgaccggca cgttagcggg cacgcatatg atcacggctc gtctggctaa
     3421 cagcaatgtc agcgatgcac agccaatgac gtttgtggcg gataaagaca gagcggttgt
     3481 cgttctgcaa acatcgaaag cggaaatcat tgggaatggc gtggatgaga caactctgac
     3541 agcaacagtg aaagatccgt cgaatcatcc ggtggcggga ataacggtga ccttcaccat
     3601 gccacaggac gttgcggcaa actttaccct cgaaaataac ggcattgcca tcacccaagc
     3661 caatggggaa gcgcatgtca cgctgaaagg taaaaaagcg ggtacgcata cggttaccgc
     3721 aacgctgggt aataacaata ccagtgattc gcagccggta acgtttgtgg cggacaaaac
     3781 ctcggctcag gttgtcctgc agatgtcaaa agatgagatc acaggtaatg gcgtcgataa
     3841 cgcaacgcta actgcaacgg ttaaagatca gttcgacaat gaggtgaata atcttccggt
     3901 aacattcagc tcagcctctt caggactcac cctgaccccg ggagtaagta ataccaatga
     3961 gtctggcatc gcgcaggcca ctctcgcagg cgttgccttt ggtgagcaga cggtcactgc
     4021 atcactggct aataatggtg ccagcgacaa caaaactgtg cattttattg gcgacacagc
     4081 agcggcaaaa attatcgagt tgacgcctgt cccagacagc ataatcgccg gtaccccgca
     4141 gaacagctcc ggcagcgtca tcaccgccac agtcgttgat aataatggct ttccggtgaa
     4201 aggtgtgact gtgaacttca ccagcagaac aaactctgcc gaaatgacga atggcggcca
     4261 agccgtaacg aacgaacagg gtaaggctac cgtcacttat accaataccc gctcctcgat
     4321 agaatcagga gcgagaccgg ataccgttga ggccagtctg gaaaatggta gctccacgct
     4381 tagcacatca attaatgtca acgctgatgc gtctacggca catctcacct tgctacaggc
     4441 actttttgat acagtctccg caggcgacac taccaatctg tatattgagg tgaaggataa
     4501 ttacggcaac ggtgtacccc agcaggaggt aaccctcaga gtatcaccaa gtgaaggcgt
     4561 gacccccagt aataacgcta tatatactac caaccacgac ggcaattttt acgcaagctt
     4621 taccgctaca aaagccgggg tttatcaagt gacggcaacc ctcgaaaatg gcgattcgat
     4681 gcaacaaaca gtgacctatg tgccgaacgt cgcgaatgcc gaaatcacgc tggcagcctc
     4741 gaaggatccg gtgattgccg acaataacga tctcacgaca ctaacagcaa cagtcgctga
     4801 tacagagggc aatgcgatag ccaacactga agtaacattt actctgccgg aagatgtgaa
     4861 ggcgaacttc acgctgagcg atggcggtaa agcgattact gatgctgaag gcaaagcgaa
     4921 agtcacgctg aaaggtacaa aagcaggcgc tcatactgtt acagcatcga tgactggcgg
     4981 taagagtgag cagttggtgg tgaactttat tgcggatacg ctcagtgcgc aggttaatct
     5041 taacgttacc gaggacaact ttatcgccaa taacgttggg atgaccacac ttcaggcaac
     5101 agtgactgat ggaaacggca acccgttagc caatgaggcg gtgacattca cgctaccggc
     5161 agatgtgagc gcaagcttca ctctcggaca aggcggttcc gccattactg atatcaacgg
     5221 caaggctgaa gttacactga gcggtacaaa atccggcacc taccccgtga cagttagcgt
     5281 gaacaattat ggtgtcagtg atacgaaaca ggtgactttg attgccgatg ctggtaccgc
     5341 aaaactagcc tccttaacct ctgtatactc attcgtcgtc agcacgaccg agggcgcaac
     5401 catgacggca agcgtcactg acgctaacgg caacccggta gaaggcataa aagttaattt
     5461 ccgcggaacc tccgtcacgc taagcagcac cagcgttgaa acggatgatc ggggtttcgc
     5521 tgaaattctt gtgacaagca ccgaggtcgg actgaaaaca gtttcagcct ctctggcaga
     5581 taaacctact gaagtcatct cgcgattact gaatgccagt gcagatgtta attctgcgac
     5641 gattaccagt ctggagatac cggaaggtca ggtaatggtc gcacaagacg tagcagttaa
     5701 agctcacgtt aacgaccagt ttggcaaccc ggttgcgcat caacccgtga cattcagtgc
     5761 agagccatcc tcgcaaatga tcatcagcca gaatacggtc tctactaata cgcagggtgt
     5821 agccgaggtc accatgacgc ccgaaagaaa cggttcgtat atggtgaaag catccctgcc
     5881 gaatggagcc tcacttgaga aacaactgga ggctattgat gaaaaactga cactcacggc
     5941 gtccagtccg cttatcggtg tctatgcccc tacaggcgct actctgacgg caacgctaac
     6001 ctctgcaaat ggcactccag tggagggtca ggtcatcaac tttagcgtaa cgccagaagg
     6061 ggcgacgtta agtggcggaa aagtgagaac taactcttca ggtcaggctc cagtcgtttt
     6121 gaccagcaat aaagtcggta catatacggt gactgcatct ttccataacg gcgtaacaat
     6181 acagacacag acaaccgtga aagtcactgg caactcaagc accgcccatg ttgctagctt
     6241 tatcgctgat ccatcgacta tcgccgccac caacactgat ttaagtacct taaaggcaac
     6301 ggttgaggat ggcagtggta acctgatcga aggtctcact gtgtacttcg ccttaaaaag
     6361 cggctctgcc acattaacgt cattaacagc ggtgaccgat caaaacggaa tcgcgacaac
     6421 aagcgtgaaa ggagcgatga caggtagcgt cacggtaagc gcagtcacga ccgctggtgg
     6481 aatgcaaaca gtagatataa cgctggtggc tggcccggca gacacctcgc agtccgtcct
     6541 taagagcaat cggtcatcac tgaaagggga ctataccgat agtgctgaat tacgtcttgt
     6601 tctgcacgat atatcaggca atccgatcaa agtttctgaa gggatggaat ttgtgcaatc
     6661 aggtactaac gtgccctata taaaaattag cgcaattgat tacagtctaa atatcaacgg
     6721 tgattacaaa gccactgtta caggaggcgg agagggtatc gcaacgctga tccctgtatt
     6781 gaatggtgtt catcaagctg gtctgagtac cacaatacaa ttcactcgcg cagaagacaa
     6841 aataatgagc ggtacagtat cagtcaatgg tactgaccta ccgacaacta cattcccttc
     6901 gcaggggttc accggggcgt attatcagtt gaataatgac aactttgccc caggaaaaac
     6961 ggcggctgat tatgagtttt caagctctgc ctcctgggtc gatgttgatg ctaccggtaa
     7021 agtgacattt aaaaatgtcg gcagctattc ggaaaggatt acggcgacgc caaaatcagg
     7081 aggccctagc tatgtatacg aaatccgtgt gaagagttgg tgggtgaacg ccggcgaggc
     7141 tttcatgata tacagccttg ctgaaaattt ttgcagcagc aatggctaca cgctccccag
     7201 agcaaactat ttaaaccact gtagttcccg aggcatcggg tcactgtaca gtgaatgtgg
     7261 agatatgggg cattacacga ctgacgctgg ttttcaatca aatatgtatt ggtcatctag
     7321 tcccgcaaac tcaagcgaac aatacgtagt ttccctggca acaggtgatc aaagcgtatt
     7381 tgaaaagctt gggtttgctt atgcgacatg ttataaaaac ctgtgatttt ccgttataat
     7441 ttcttaaaga gtgttccatt cggggcactc ttttttacat tccctctaca gaactagccc
     7501 ttctctcaca gaaaatgaat aaataaaaat gctgcaccgc cagaatcgcg ttcgatgtga
     7561 ctatgatcat tttcggtgag tactgctttg atctaaaggc tggttttctg tgatgattga
     7621 ccagcctcag agtattgatg acaccaccgt tcataacaga tcggcattac tatgcataag
     7681 tactgtctca tattctcgcc aaaacacttt ttaatgcctt tgcgaattca tccagactta
     7741 tattgtcaat tttttgccca acgacgactg actccgagac atcggccatt gcgggccatc
     7801 gcgttttgta atcagcataa attgcaacaa caggggtatt atacgcactt gccatatgta
     7861 atattgaggt gccaggagtt attgcgatat aagcatcttt tatgattgct gctgaacgtt
     7921 taatcgaggg tggtaaggat aaacggtaga cattattata acaatccact aatgccctgg
     7981 ctttatcttc acctttgggt ccatgaacga tcactattgg tatatctgtt tcacgttgaa
     8041 tttttgctat tagattttct gcaatcgata atgaaaatgt acgttcctgc gagctacctt
     8101 ctaaattgaa cgcaatgtaa gatcctaacg atcgcatttc ctcgcgcacc tcatcgagta
     8161 catcctcact tagtggcaat tcatatattg gcctgactgc tggaaaaccc gcctcacgca
     8221 tcataaatgc ccatgtcata ggtacgggag cccggagttt ctgatccata cgggacgcgt
     8281 tcttgcacaa aggggagaag caattcatgg ttataccaac aacctgaaaa ttcgtttttg
     8341 ctttcaactg actgataaat aacatcgttt tcaggttctt tttacgcatc ccctcaatgc
     8401 aaagatccgg cgtaccgtat tgctgtgtta tgttctttgc taaatctttt atttctttta
     8461 atgttgcgtg atcctgcata gtcattgtga ctaatgttaa tttagtctgt tcaagtttaa
     8521 gcgcattaaa gacttctaaa ttaattgtcg acgttacaat taaaagatgc ttaattttat
     8581 gcaattcaag cgcc
//
LOCUS       DAPHBJ010000182         8589 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.184, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000182 DAPHBJ010000000
VERSION     DAPHBJ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.184"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(221..1813)
                     /locus_tag="RHZ76_003706"
     CDS             complement(221..1813)
                     /locus_tag="RHZ76_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HDV2814275.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            2032..2952
                     /gene="ldtB"
                     /locus_tag="RHZ76_003707"
     CDS             2032..2952
                     /gene="ldtB"
                     /locus_tag="RHZ76_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDV2814276.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(3011..4129)
                     /locus_tag="RHZ76_003708"
     CDS             complement(3011..4129)
                     /locus_tag="RHZ76_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HDV2814277.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(4126..4593)
                     /gene="mntR"
                     /locus_tag="RHZ76_003709"
     CDS             complement(4126..4593)
                     /gene="mntR"
                     /locus_tag="RHZ76_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HDV2814278.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            4779..4907
                     /gene="mntS"
                     /locus_tag="RHZ76_003710"
     CDS             4779..4907
                     /gene="mntS"
                     /locus_tag="RHZ76_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HDV2814279.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            5179..6762
                     /gene="opgE"
                     /locus_tag="RHZ76_003711"
     CDS             5179..6762
                     /gene="opgE"
                     /locus_tag="RHZ76_003711"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDV2814280.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(6811..7326)
                     /gene="ompX"
                     /locus_tag="RHZ76_003712"
     CDS             complement(6811..7326)
                     /gene="ompX"
                     /locus_tag="RHZ76_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HDV2814281.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(7379..7444)
                     /locus_tag="RHZ76_003713"
     CDS             complement(7379..7444)
                     /locus_tag="RHZ76_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814282.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            7679..8566
                     /gene="rhtA"
                     /locus_tag="RHZ76_003714"
     CDS             7679..8566
                     /gene="rhtA"
                     /locus_tag="RHZ76_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HDV2814283.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         2131 a         2255 c         2045 g         2158 t
ORIGIN      
        1 tttatattgg tcaatctgtg tgggttaaaa caaagaacag tgtgggaaca attattgatg
       61 ttttttggca ttttaaaaat gagatgcctg tttattttat aaactttgaa ggtaaacgct
      121 catccaggcg atattcagag agtgagttgt ctcacccaca taatatactt tgaatgtagc
      181 gcggccccac agagattacc tctgtagagc cagcataata ttactcgatc cctttactac
      241 gcaggtaatc ttcgtaatta ccgctaaagt cgatcacgcg ttccggggtg atttccagaa
      301 tgcgggtcgc cagggagctt acgaactcac ggtcgtgtga aacaaagatc agcgtgccct
      361 gatacagttc cagtgccatg ttcagcgact caatggattc catatccagg tggttggtcg
      421 gttcgtccat gatcagaatg ttcggcttct gcatcattaa cttaccaaac agcatccgcc
      481 ctttttcccc accggaaagc actttagctg gctttttgat gtcgtcctgg ctgaacagca
      541 aacgaccgag aatactgcgt accgcctgct cgtcatcgcc ttcctgcttc cactggctca
      601 tccattcgaa cacggtcaga tcattttcaa actcatattc gtggtcctga gcatagtaac
      661 caatgcgcgc gttctcagac cattttacgg tgccgctgtc cggttgcaga tcgcccacca
      721 gcgttttcag cagcgttgat ttaccgacgc cgttggtacc cagtaccgcc agtttttcac
      781 ccacttccag cagcaggttg agatttttaa acagcggacc gttatcaaac cctttggtca
      841 gaccttccac ttccagcgcg ttacggaaca gtttcttatc ctgttcaaaa cggatgaacg
      901 ggttctgacg gctggaggct ttcacctctt ccagtttgat tttatcaatc tggcgcgcgc
      961 gcgaagttgc ctggcgagat ttcgaggcgt tggcgctaaa gcggctaacg aaagattgca
     1021 actcagcaat ctgcgctttc ttcttggcgt tatcggccag cagacgttca cgcgcctggg
     1081 tcgccgccgt catgtactca tcgtagttac ccggataaac gcgcagctcg ccgtaatcca
     1141 gatccgccat gtgggtacag accatgttaa ggaagtgacg gtcgtgcgag atgatgatca
     1201 tggtgctgtc acgctcgttc agcacctgtt ccagccagcg aatggtgtcg atgtcgaggt
     1261 tgttggtcgg ttcgtcgagc aggagaatat ccggatcagc aaacagcgcc tgcgccagaa
     1321 gcacacgcag cttccagcca ggagcaactt cactcatcgg gccgtagtgc tgttccactg
     1381 gaattcccac gccaagcaac agttcaccgg cgcgagcttc cgcagagtaa ccgtccattt
     1441 cgccgtattt aacttccaga tcggccactt tatagccgtc ttcttcactc atttccggca
     1501 aagcatagat gcggtcgcgc tcctgcttca cttcccacaa ctctttatgc cccatgatca
     1561 ccgtatccag cacagtgaac tcttcaaagg caaactgatc ctgacgcagt ttaccaatgc
     1621 gctcgttggg atcgagggaa acgttaccca gcgtcggctc aaggtcgccg ccgaggatct
     1681 tcataaaggt ggatttacca ctaccgttcg cgccaatcag gccgtaacgg ttgccgccgc
     1741 caaatttgac ggaaatgttt tcaaacaacg gcttactgcc gaactgcatg gtgacgttac
     1801 tggaaactaa cacaggcgta tcctgaaaag agatatgaca aaccgcgcat tatgccataa
     1861 gtctgaaaga agatcctgct cctcgcttaa ggtctacact caagccaaag gcaaaaaatg
     1921 tgatttcgta cacatctgat ttcactgtga gctggaatga acttataatg cgcttccaat
     1981 actctaatat tctcaaccca atggcctgcc aggcacaaaa tctcgcttaa catgaatatg
     2041 aaattgaaaa cattattcgc agcggccttc gctgttgtcg gcttttgcag taccgcctct
     2101 gcggtaactt atcctctgcc aaccgacggg agtcgcctgg ttggtcagaa tcaggtgatc
     2161 accattcctg aaggtaacac tcagccgctg gagtattttg ccgccgagta ccagatgggg
     2221 ctttccaata tgatggaagc gaacccgggt gtggatacct tcctgccgaa aggcggtact
     2281 gtactgaaca ttccgcagca gctgatcctg ccggataccg ttcatgaagg catcgtcatt
     2341 aacagtgctg agatgcgtct ttattactat ccgaaaggga ccaacaccgt tatcgtgctg
     2401 ccgatcggca ttggtcagtt aggcaaagat acgcctatca actggaccac caaagttgag
     2461 cgtaaaaaag caggcccgac ctggacgccg accgccaaaa tgcacgcaga gtaccgcgct
     2521 gcgggcgaac cgcttccggc tgtcgttccg gcaggtccgg ataacccgat ggggctgtat
     2581 gcactctata tcggtcgcct gtatgctatc catggcacca acgccaactt cggtatcggc
     2641 ctgcgtgtaa gtcatggttg tgtgcgtctg cgtaacgaag acatcaaatt cctgttcgag
     2701 aaagtaccgg tcggtacccg cgtacagttt attgatgagc cggtaaaagc gaccaccgag
     2761 ccagacggca gccgttatat tgaagtccat aacccgctgt ctaccaccga agcccagttt
     2821 gaaggtcagg aaattgtgcc aattaccctg acgaagagcg tgcagacagt gaccggtcag
     2881 ccagatgttg accaggttgt tcttgatgaa gcgattaaaa accgctccgg gatgccggtt
     2941 cgtctgaatt aatcttcaaa acttaaagca aaaggcggac tcataatccg ccttttttat
     3001 ttgccagacc ctagttggcc gggagtataa ctaacaaaac atatcccacc aacgccgccc
     3061 acaacagcat cggtattgaa tagagatgga aacgccacca gatgcggcga tcgttcgcca
     3121 tacgtagcgc aatcaaattt gccagcgatc cgggtaataa cccaaagcca cctacgttta
     3181 ccgcccatac cagtaataaa gacggcggca catagttcag caacaatatg gtactcggca
     3241 cgttactgat cacctgcgat aaaccgattg ccgttaacca taacccgggt tcagatagat
     3301 gactcacgtt acccaacacg ccttgcaacg ctggaagctg ggtcagtaaa tggacgtcga
     3361 taaacatcgc cataaacacc agcagcagcg tccagtccac actgagcacc acgcgacgtg
     3421 ccagcagcgc aaagcctgcc gccacaatca ccagtcccca cagctcttgt ttgaactcca
     3481 gcgccgtcag aaagacgata tacagcccca gacaactcca caccagccgc ggtttccact
     3541 ccggtgtttg cacccccgta tggtattgca gcgcctttcc agggaaacaa caccagcaca
     3601 ggagcaggag cgtcagcatc attgcgccag ccagcggtgc catttgggca ataaatccgg
     3661 caaacgaaag accagaacgt ccccagataa gaatattttg cgggttgcca attggcgtca
     3721 gtagcgaacc agcgttgact gccagcgcct caaaaataat cagccgatta accgggatct
     3781 cacacagtct ttttagcgtg atagtcagcg gaacaacaat aaacagcgcg acatcgttgg
     3841 tcagaaaggt agaaagcagc gccgccgcca gcaccataaa catcgccagc cgacgctccg
     3901 tagcaaagcg gcgcaccatt ttgcgcccca gcacatcaaa ataaccgctt aactccacac
     3961 ctttggtcag cagcatcagg ccgcttaagg tgatgatggt gtgccagtcg atagcagcag
     4021 gccaggattt cggtgcaaag ggcacgaaaa agcttaatcc gataccaaca agaattaata
     4081 actgaaaaaa acgatcgcct tgcagcgtgc gtaaaaaagg caggctcatt tggcaccgtg
     4141 tttctgggta aacaaacgaa aagcgtcgag cgtctcttca ctaacatggt gctccatgcc
     4201 ttccgcgtcg cgacgggcga tttccggact gacgcccaac accaacaaga aattttcgac
     4261 tatctgatgt cgctcgcggc tttcctgcgc cagcttctct ccttctgccg ttaaaaacac
     4321 gccacgccag gggatcattt caatcagccc catggttgcc agccgcttaa gcattttagc
     4381 caccgtcggt tgcgaaactc ccagacgagc agccatgtcc acctgacgag cttcccccac
     4441 ttccctgatc aagtcagaaa tcagctcaac gtaatcatca ataagctcgc gccgatgcgc
     4501 ctctctgacc tggcggaacc cttcaacgtg ctcttccacg ttcactaact gcgtcacttt
     4561 ttttgctgtt ggcgtacctg cgcgacgact cattgtgctt cctcattatg gtgacgcata
     4621 catagcgttc tcatttcgtg cgcacattgt aaaccagagt tgcgaaggta caaaaaatta
     4681 acgttttagc aatagctata taatatagcc tgtgctatat ctgtatgtaa tgcaatcatc
     4741 cctcaaggat cgacgggatt agcaagtcag gaggtcttat gaatgagttc aagaggtgta
     4801 tgcgcgtgtt tagtcattct ccctttaaag tacggttaat gctgctctct atgttgtgcg
     4861 atatggtcaa caacaaaccg cagcaagata aaccttccga taaatagcgg cgtcgcggta
     4921 cgccgcttca ctcctgcttt catgcaggca taacgcgttt tggtctgaaa aaccccactt
     4981 tttgtcggat ttgcaatccc cttcgcaaaa gatttgttcg tcagtagttg acctgaacgg
     5041 cggctcgctc tatcttcttg cagccctgcg tatattgcgg ctcgcggatg cggacccctt
     5101 tccactcttc acgcactctt gcaggtattg acccttgacg ccagggtaag cacatggcgt
     5161 ttgttacgat agtggcatat gaatttaacc ctcaaagaat cgcttgttac ccgtagccgg
     5221 gtatttagcc cgtggactgc gttctacttt ttacagtcgc tattaattaa cctcggctta
     5281 ggttacccct tcagtttgct ctacaccgct gcgtttacgg ctattttgct tttgctatgg
     5341 cgaacattgc ctcgcgtaca aaaagttctg gtcggtgtca gttcgctggt ggcggcttgt
     5401 tatttccctt ttgctcaggc ctacggcgcg cctaatttca atacattgct ggcattgcac
     5461 tccaccaata tggaagagtc gaccgaaatc ctgacgattt ttccgtggta cagctacctg
     5521 gtcggcttat ttatttttgc gctcggcgta atagcaatca ggcgaaaaaa agagaatgaa
     5581 aaagcgcgct ggaatacctt cgacagcctg tgtctggtat tcagtgtggc gacatttttt
     5641 gttgctcccg tgcaaaacct ggcctggggt ggcgtattta aactgaaaga tactggctat
     5701 ccggtatttc gttttgctaa ggatgtcatc gtcaataata acgaggtgat tgaagagcaa
     5761 gaacggatgg caaaactttc cggaatgaaa gatacctgga cggtcactgc cgttaagccg
     5821 aagtatcaga cctatgtggt ggtgatcggt gaaagcgcgc gtcgcgatgc cctcggtgcc
     5881 tttggcggtc actgggacaa taccccgttt gccagcagcg ttaacggttt gatatttgct
     5941 gactacattg ccgccagtgg ctccacgcag aaatcgcttg gcttaacgct caatcgcgtt
     6001 gtcgatggca aaccacagtt tcaggataac tttgtcaccc tggcaaatcg cgcgggcttc
     6061 cagacctggt ggttttccaa ccagggtcaa atcggcgaat acgataccgc tatcgccagc
     6121 atcgccaaac gagcagatga agtgtacttc ctgaaagaag gtaattttga agcagataaa
     6181 aacaccaaag acgaagcgtt actggatatg accgctcaag tgctggcgca agagcactcg
     6241 caaccgcagc tgattgttct acatctgatg ggctcacatc cgcaggcctg cgacaggaca
     6301 caaggaaaat acgaaacctt tgtgcaatcg aaagaaacgt cgtgctatct ctataccatg
     6361 acgcaaacgg acgatttact gcgcaagctg tacgatcagt tacgcaacag cggcagcagc
     6421 ttctcgctgg tttacttttc tgaccacggt ctggccttta aagagcgcgg taaagacgtg
     6481 caataccttg cccatgatga taaatatcag caaaatttcc aggtgccttt tatggtcatt
     6541 tccagcgacg ataaagcgca tcgtgtgatt aaagcccgcc gctcagccaa tgacttctta
     6601 ggctttttct cccagtggac ggggattaaa gcgaaggaaa ttaacatcaa atacccgttt
     6661 atatctgaga agaaagccgg gccgatatac atcaccaact tccagttaca gaaggtggat
     6721 tacaaccatc tcggaaccga tattttcgac ccgaaacctt aaaaacaaaa atccgccccg
     6781 agaggcggat tttttatatc accaaagtga ttagaagcgg taaccaacac cggcaatcca
     6841 ggtgcctacg tcaacgctac gaatacggct ctgctcgtaa gagaagtcca gagcaacgtt
     6901 ttccatcggg ttgaactgca gacccgcacc gtaggagaaa ccgtagtcgc tggtgtcgtg
     6961 tttgtaggtc gggtattcag tggtctggaa tttaccataa cccacaccca ctacaccgta
     7021 gatgcttgcc cagtcgttaa tgcggtaagc cggaccagca gtgatgccgt agtactggtt
     7081 tttgttgtag tcaccagagc ttgcagtacg gcttttctcg gtgtaagtga aagaaccgat
     7141 cacacccagc gggctgttgt cttcttcata gcggtatttc aggttgaaac cgcccatttt
     7201 gttcatttgg ccctgagcgt cgctctgtgc gtaaccgcca gttacagtag aagtcgcagc
     7261 tacggaagta cctgcggtga aagccagaac tgcggccagt gctgaaagac atgcaatttt
     7321 tttcataacc acctcaaatg tgattcaaat aagtcctaag ttttaaatat atcaaaaatt
     7381 aatgggaaac tcttcgcgat ttgtgatgtc taacgggcca tttcatgtaa cagaacgttt
     7441 ccatacaccg ctatccatct aaatttaaat cactttttca gagaactgcg taagtattac
     7501 gcatgttttc cctgtcattc atccagatta ttcctaatca ccagactaat gattccatca
     7561 atactggcgc attttagtca aaacggggga aaattttttc aacaaatgct caaccagcat
     7621 tgggtatatc cagtacactc cacgctttac ttaagtctag atatttgtgg gagaaaggat
     7681 gcctggttca ttacgtaaaa tgccggtctg gttaccaata gtcatattgc tcgttgccat
     7741 ggcgtctatt cagggtggag cctcgttagc taagtcactt tttcctctgg tgggcgcacc
     7801 gggtgtcact gcgctgcgtc tggcattagg aacgctgatc ctcatcgcgt tctttaagcc
     7861 atggcgactg cgctttgcca aagagcaacg gttaccgctg ttgttttacg gcgtttcgct
     7921 gggtgggatg aattatcttt tttatctttc tattcagaca gtaccgctgg gtattgcggt
     7981 ggcgctggag ttcaccggac cactggcggt ggcgctgttc tcttctcgtc gcccggtaga
     8041 tttcgtctgg gttgtgctgg cggttcttgg tctgtggttc ctgctaccgc tggggcaaga
     8101 cgtttcccat gtcgatttaa ccggctgtgc gctggcactg ggggccgggg cttgttgggc
     8161 tatttacatt ttaagtgggc aacgcgcagg agcggaacat ggccctgcga cggtggcaat
     8221 tggttcgttg attgcagcgt taattttcgt gccaattgga gcgcttcagg ctggtgaagc
     8281 actctggcac tggtcggtta ttccattggg tctggctgtc gctattctct cgaccgctct
     8341 gccttattcg ctggaaatga ttgccctcac ccgtttgcca acacggacat ttggtacgct
     8401 gatgagcatg gaaccggcgc tggctgccgt ttccgggatg attttcctcg gagaaacact
     8461 gacacccata cagctactgg cgctcggcgc tatcatcgcc gcttcaatgg ggtctacgct
     8521 gacagtacgc aaagagagca aaataaaaga attagacatt aattaaattt acatttctgc
     8581 atggttatg
//
LOCUS       DAPHBJ010000183         8561 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.185, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000183 DAPHBJ010000000
VERSION     DAPHBJ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.185"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(175..2166)
                     /gene="tkt"
                     /locus_tag="RHZ76_003715"
     CDS             complement(175..2166)
                     /gene="tkt"
                     /locus_tag="RHZ76_003715"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDV2814284.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2444..3202
                     /gene="loiP"
                     /locus_tag="RHZ76_003716"
                     /gene_synonym="yggG"
     CDS             2444..3202
                     /gene="loiP"
                     /locus_tag="RHZ76_003716"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HDV2814285.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDEQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(3408..4328)
                     /gene="speB"
                     /locus_tag="RHZ76_003717"
     CDS             complement(3408..4328)
                     /gene="speB"
                     /locus_tag="RHZ76_003717"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDV2814286.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(4466..6442)
                     /gene="speA"
                     /locus_tag="RHZ76_003718"
     CDS             complement(4466..6442)
                     /gene="speA"
                     /locus_tag="RHZ76_003718"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HDV2814287.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(6451..6582)
                     /gene="yqgB"
                     /locus_tag="RHZ76_003719"
     CDS             complement(6451..6582)
                     /gene="yqgB"
                     /locus_tag="RHZ76_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HDV2814288.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            6718..6933
                     /locus_tag="RHZ76_003720"
     CDS             6718..6933
                     /locus_tag="RHZ76_003720"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814289.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            7237..8391
                     /gene="metK"
                     /locus_tag="RHZ76_003721"
     CDS             7237..8391
                     /gene="metK"
                     /locus_tag="RHZ76_003721"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDV2814290.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         2079 a         2307 c         2206 g         1969 t
ORIGIN      
        1 tagatataac aacaaatgtc aatacgcata tcgtgatgca tattacataa ttgatattta
       61 ttgcatataa atatttgtga tctacaacac gccttatcta ttgcttgtcc gcaaacggac
      121 atatcaaggt aataaaaaag gtcgccgaag cgaccttttt acccgaaatg ctaattacag
      181 cagttctttt gctttcgcaa caacgttatc aacagtgaag ccgaactctt caaacagcag
      241 ctctgccgga gcagattcac cgaaggtggt cataccgacg atagcaccgt tcaggccaac
      301 atacttgtac cagtagtcag caatacccgc ttctacagca acgcgtgcag taaccgcttt
      361 cggcagtacg gattcacggt aagcagcatc ctgcttgtca aatgcgtcgg tagacggcat
      421 ggacaccacg cgcgctttca cgccttcggc agtcagtttt tcgtaggcag caacagccag
      481 ttcaacttct gaaccggtag cgatgaaaat cagttccggc tgaccggcgc agtctttcag
      541 cacataacca ccgcgcgcga tgtttgccag ttgctcttca gttcgttcct gctgcgccag
      601 gttctgacgg gagaggataa gcgcagtcgg gccgtcctga cgctcaacgc cgtatttcca
      661 cgcgaccgcg gattcaacct ggtcacacgg acgccatgta gacatgttcg gggtcacgcg
      721 cagagaagcg acctgctcaa ccggctggtg agtcgggcca tcttcgccca gaccgatgga
      781 gtcgtgggtg taaaccatca cctgacgctg tttcatcagc gcagccatac gtacggcgtt
      841 acgtgcgtat tccacgaaca tcaggaaggt ggaggtgtac ggcaggaaac caccgtgcag
      901 ggagataccg ttagcaatcg cggtcatacc gaactcgcga acaccgtagt ggatgtagtt
      961 acctgcagca tcttcgttga ttgctttaga accagaccac agggtcaggt tagacggtgc
     1021 caggtcagca gagccgccga ggaattcagg caacagcggg ccgaacgctt cgatagcatt
     1081 ctgcgacgct ttacggctgg cgattttcgc cggattagcc tgcagtttag cgataaactc
     1141 tttcgctttg gcgtcgaagt cagacggcat ttcgcctttc atacggcggg taaattcagc
     1201 cgcttcctgc ggataagctt tcgcgtaagc cgcaaacttc tcattccatg cagattcttt
     1261 cgcctggcct gcttctttcg catcccactg agcatagatt tcagacggga tttcgaacgg
     1321 cgcgtatttc cagcccagct gttcgcgggt cagggcaatt tcagcgtcgc ccagcggcgc
     1381 accgtgggag tcgtgggtac cggctttgtt cggggaaccg aaaccgatga tggttttgca
     1441 catcagcagg gacggtttgt cagtcactgc gcgcgcttct tctactgcgc gtttgatgga
     1501 tgccgcgtca tgaccgtcga tgtcgcgaat aacgtgccag ccgtaagctt cgaaacgcat
     1561 tgcggtgtcg tcagtgaacc agccttcaac gtgaccatcg attgagatac cgttgtcatc
     1621 gtagaacgca atcagtttac ccagcttcag cgtacccgcc agagagcaaa cttcgtggga
     1681 gatgccttcc atcatgcagc cgtcgcccat gaaggcgtag gtgtagtggt cgacaatgtc
     1741 gtgacctgga cggttaaact gcgccgccag cgttttttct gcaatcgcca taccgactgc
     1801 gttggcaata ccctgaccca gcggaccggt ggtggtttcc acaccagcgg tataacctac
     1861 ttccgggtgg cccggagttt tagagtgcag ctgacggaag tttttcagtt cttccatcgg
     1921 cagatcgtaa ccggtgaggt gcagcaggct gtagatcagc atggagccgt ggccgttgga
     1981 cagcacgaag cggtcacggt cagcccagga cggattctgc gggttgtgtt tcaggaaatc
     2041 acgccacagg acttcggcaa tgtcagccat acccataggg gcacccgggt gaccggattt
     2101 ggctttctgt actgcgtcca tgctcagcgc acgaatagca ttggcaagct ctttacgtga
     2161 ggacattttg actccagatc ggatgatgaa gggcacgccc ttaacgactt gacgacagcg
     2221 cgttttgggc tacgccggaa aatttgccaa caatttaccg caagccgcgc gtcatgtaca
     2281 tggaacatcc ttttgctgct tcagaaatct ctggatcatg ctcgcatgtt gcgcaatcta
     2341 ctcgcccgtc cgttgcgctt ttccttatac tgagactgag cgtcgattca cctgcaaacg
     2401 gcgcattttt agaataatcc tgaccttgtg cggaagagaa aacatgaaaa ttcgcgcctt
     2461 attggtagca atgagcgtgg caacggtact gaccggttgc cagaatatgg actccaacgg
     2521 actgctctca tcaggagcgg aagcttttca ggcttacagt ttgagtgatg agcaggtgaa
     2581 aaccctgagc gatcaggcat gtcaggagat ggacagcaag gcgacgattg cgccagccaa
     2641 tagcgaatac gctaaacgtc tgacaactat tgccaatgcg ctaggcaaca atatcaacgg
     2701 tcagccggta aattacaaag tgtatatggc gaaggatgtg aacgcctttg caatggctaa
     2761 cggctgtatc cgtgtctata gcgggctgat ggatatgatg acggataacg aagtcgaagc
     2821 ggtgatcggt cacgaaatgg ggcacgtggc attaggccat gtgaaaaaag gaatgcaggt
     2881 ggcacttggt acaaatgccg tgcgagtagc tgcggcctct gcgggcggga ttgtcggcag
     2941 tttatctcaa tcacaactcg gtgatctggg cgagaaatta gtcaattcgc aattctccca
     3001 gcgccaggaa gcagaagccg atgattattc ttacgatctt ctgcgccaac gcggcatcag
     3061 cccggcaggt cttgccacca gctttgaaaa actggcaaaa ctggaagaag gtcgccaaag
     3121 ctcaatgttt gacgaccatc ctgcatccgc cgaacgcgcc cagcatattc gcgatcgtat
     3181 gagcgcagat gggattaagt aaagcctggt ggtgtcgaac gtaggtcaga taaggcgttc
     3241 acgccccttc tgacatgaat ggcacatttg ttaccctgtg cgcattgccg gatgcgatgc
     3301 tggcgcatct tatccggcct acgggtgccg aacgtaggtc ggataaggcg ttcacgccgc
     3361 atccgacatt aatggcacgt tttacccgtg cgcatcgcat ctggtgctta ctcgcccttt
     3421 ttcgccgcct gaatatagag catctccagc gccagcgtcg ccgcagccag agcggtgatt
     3481 tccgactgat cgtatgccgg agccacttcc actacgtcca tcccaacgat gttgagatct
     3541 ttcaggccgc gtaccagttt aatggcgcga tcggaggtca ggccgccaat cactggcgta
     3601 ccggttcccg gcgcgaaagc aggatccagg cagtcgatat caaaggtcag gtaaaccggc
     3661 atatcaccca caatctgttt cacttgggcg ataacgtcat ccacgctgcg atcgttcacc
     3721 tggcaggcgt ccagcacggt aaaaccgttg tctttatcaa actcggtacg aataccaatc
     3781 tgcacggaat gattcgggtc gatcagacct tctttcggcg cggtatagaa catggtgccg
     3841 tggtcaaatt cacaaccgtt cgcgtaggta tcggtgtggg cgtcaaagtg taccagcgcc
     3901 attttgccga aatgcttcgc atgagcacgc agcagcggca gcgtaacaaa gtggtcacca
     3961 ccgaaggaga gcatacgctt accggcagcc agcagcttct cggcgtgcgc ctgcagcttc
     4021 tcgctcatct cacgggcatc gccaaaggca tataccagat cgccgcagtc cacgacgttc
     4081 agacgctcac gcatgtcgaa attccacggg aagcggttgt gttcccaggc cagattcgtc
     4141 gaaacctgac ggatcgctgc cggaccgtgg cgaccacccg cacggccaga ggtggccatg
     4201 tcgaacggca cgccggtgat cacccagtcg gcatcgctgt catacggctg gaagttcatc
     4261 ggcaggcgta aaaaaccaaa ggcgttggaa accagtgagt tatcgtattg atgacctaag
     4321 gtgctcatgg atggactcct attacaaagt cgatataaaa aaacccttcc gcgtcgttag
     4381 gcccgacgag gaagggttgg atttgtcaca ataaattgtg gcggattatc accgctaaat
     4441 taagcggatt caagtaacac aggacttact catcttcaag ataagtataa ccgtacaaac
     4501 ctgcctcgaa ctcttcaagg aactgttgtt gcagttcagc atcaagatcg gttttcttca
     4561 cttgatcgcg gaactgggtt aacagcgttt tcggatcgag ctgtacatat tgcagcatgt
     4621 ccgccacggt atcgccttcg tcagacagtt ctacttctac gctaccgtca gggaacacga
     4681 acacgtcaac cgcttcggta tcaccgaaca ggttgtgcat attgccaagg atctcctgat
     4741 atgcgccgac cataaagaaa ccgagcatcg gcggattctc tggatcgtac tccggcattg
     4801 gcattgtcgt ggcaatacca tcaccatcaa tatagtggtc gatagcaccg tcagagtcgc
     4861 aggtaatatc cagcagcaca gcgcgacgtt ccggcacttg atccagccct tccagcggca
     4921 gaaccgggaa caactggtcg atcccccatg cgtccggcat tgactggaac agcgagaaat
     4981 tgacgtacat tttgtccgcc atacgttcct gcagttcgtc gattatcgga cgatgcgcac
     5041 ggttttgcgg atccagttgc ttctgcactt catggcacat gctcagatag agttgttctg
     5101 cccatgcacg ttcttgcagg ctaaaggtgc cggaagagta gccgatatga atgtcgtgca
     5161 gatccatctg actgtcgtgt aaccattcac gcagagaacg gcgagttccc ggttcgtgca
     5221 tctcctgcca ggtttcccac atgctttgca gcgcgcgcgg cgcatcttct gcaggcgcgg
     5281 tcggcaccgt gtattcgtta cgttccacgc cgatgatatt agacaccagc acggtgtgat
     5341 gcgcagtcac cgcacgaccc gattcggtga ttaccgtcgg atgcggcaga ccgttttctt
     5401 cacacgcatc accaatcgcc cagatgatgt tgttggcata ttcattgagg ccgtagttta
     5461 cggagcagtc agactgcgaa cgggtgcctt catagtccac gcccagaccg ccgccgacgt
     5521 cgaagcactg aatattgacg cccagcttgt gcagttccac atagaaacgc gccgattcac
     5581 gaacgcctgt ggcgatatcg cgaatattcg ccatctgcga accgaggtgg aagtgcagta
     5641 gttgcaggct gtcgagacgc ccggcttcac gcagggtttc aaccagttgc agtacctgag
     5701 tcgcagccag gccgaacttc gatttttccc cgccggagga ctgccattta cccgagccct
     5761 gcgaagccag acgtgcacgc acgcccagac gaggaacgac attcagacgt tctgcttcat
     5821 ccagcacaat ggcgatttct gacatcttct caatgaccag gtagaccttg tggcccatct
     5881 tctcgccaat taatgccagg cggatatatt cgcggtcttt ataaccgttg cagacgatga
     5941 cgctacgggt catgccagca tgtgccagca ccgccatcaa ctctgctttc gagcctgctt
     6001 ccagtcccag cggttcgccc gaatgaatca gggactcaat cacgcggcgg tgctggttaa
     6061 ctttgatcgg ataaacaagg aagtaatcgc cgttatagcc gtaggattcc cgcgcacgtt
     6121 tgaacgcggc gttaatggaa cgcaaacggt gctgcaggat ctgtgggaaa cagaacagtg
     6181 caggcagacg ctgaccctgt gcttcacgag ttttcactaa ctgcgcgaga tcgacgcgag
     6241 cttccgggac gtccgggtcc gggcacacgc tgatgtggcc cagctcgtta acgtcatagt
     6301 agttattgcc ccaccaggca atattgtaag tacgcagcat cttgctggct tcctgggagc
     6361 tcattgcaac ctcctgcatg gagcgtagta caccgtgttc gcccgctgac gaaggcaaac
     6421 ccatagacat gtcgtcagac atagcgaacc tcaaattatt ttattaagtg taaaacagtt
     6481 aacgactatc gcagcctgga actgcgataa caacccataa gcacatggat tttccagcag
     6541 tgtatgctga cgctccgact gcgcaaccgg tttctttttc ataacattat taagcacata
     6601 accgaacgta agtgtgacag ttcggcgaaa ccacgagaaa actcttgttt ttacaagagc
     6661 gcccttgttc agtcctcagt aactgtaacc agctcttgaa tcctgagaag cgccgagatg
     6721 ggtataacat cggcaggtat gcaaagcaga gatgcagagt gcggggaacg aatcttcacc
     6781 agaacggtga gacaggttaa gcagcagaca acggttcatt atttcgtatc acctccacgg
     6841 ccgcctgtta agacgaaccc acaagccaaa actctgattt caacccggct ggaagtggca
     6901 acacgaaaga aacgtcgtgt gctttttatt taagccgcgc gccgcgtttt ataccccaca
     6961 atggcagaaa attgcaaaag ataaatacgc agaatgccgg cattgtcagg aaaaatttcc
     7021 agccacgttt ttaacagaat gagacacgat tcaaaaaaaa gtggaaatcg ggtgaagaat
     7081 tgacctaaaa tagccatcca gatgttaatc catccatacc gattaacact cagactgcca
     7141 gtgtttttaa cctgcagagt cgtggtagga tccgctacca cagaaaatcc acacaacagt
     7201 ttgagctaac caaattctct ttaggtgata ttaaatatgg caaaacacct ttttacgtcc
     7261 gagtccgtct ctgaagggca tcctgacaaa attgctgacc aaatctctga tgccgtttta
     7321 gacgcgatcc tcgaacagga tccgaaagca cgcgttgctt gcgaaaccta cgtaaaaacc
     7381 ggcatggttt tagttggcgg cgaaatcacc actagcgcct gggtagacat cgaagagatc
     7441 acccgtaaca ccgttcgcga aattggctat gtacattccg acatgggctt tgacgctaac
     7501 tcctgtgcgg ttctgagtgc tatcggcaaa cagtctcctg acatcaacca gggcgttgac
     7561 cgtgccgatc cgctggaaca gggcgcgggt gaccagggtc tgatgtttgg ctacgcaact
     7621 aatgaaaccg acgtgctgat gccagcacct atcacctatg cacaccgtct ggtacagcgt
     7681 caggctgaag tgcgtaaaaa cggtactctg ccgtggctgc gcccggacgc gaaaagccag
     7741 gtgactttcc agtatgacga cggcaaaatc gttggtattg atgctgtcgt gctttccact
     7801 cagcactctg aagagatcga ccagaaatcg ctgcaagaag cggtaatgga agagatcatc
     7861 aagccgattc tgcccgctga atggctgact tctgccacca aattcttcat caacccgacc
     7921 ggtcgttttg ttatcggtgg cccgatgggt gactgcggtc tgactggtcg taaaattatc
     7981 gttgatacct acggcggcat ggcacgtcac ggtggcggag cattctctgg taaagatcca
     8041 tcaaaagtgg accgttccgc agcctacgca gcacgttatg tcgcgaaaaa catcgttgct
     8101 gctggcctgg ccgatcgttg tgaaattcag gtttcctacg caatcggcgt ggctgaaccg
     8161 acctccatca tggtagaaac tttcggtact gagaaagtgc cttctgaaca actgactctg
     8221 ctggtacgtg agttcttcga cctgcgccca tacggtctga ttcagatgct ggatctgctg
     8281 cacccgatct acaaagaaac cgcagcatac ggtcactttg gtcgtgaaca tttcccgtgg
     8341 gaaaaaaccg acaaagcgca gctgctgcgc gatgctgccg gtctgaagta atctttcttc
     8401 acctgctttc aaaggccagc ctcgcgctgg cctttttctt ttaccttacc gcggtggata
     8461 ggcattcacg ccgcatccgg caaaaagctg cccgcacaat aacatcattc ttcctcaaca
     8521 cgttttatcg caaattatca tcactactga aaccgattac a
//
LOCUS       DAPHBJ010000184         8503 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.186, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000184 DAPHBJ010000000
VERSION     DAPHBJ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.186"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            151..4174
                     /locus_tag="RHZ76_003722"
                     /pseudo
     CDS             151..4174
                     /locus_tag="RHZ76_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310144.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
     gene            4171..5571
                     /locus_tag="RHZ76_003723"
     CDS             4171..5571
                     /locus_tag="RHZ76_003723"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV2814291.1"
                     /translation="MNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPTDKMNVK
                     GDTQGNTRVRVDNIGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVYTLAKGK
                     GNDEKNWYLTSKWDGVTPADTPDPINNPPVVDPEGPSVYRPEAGSYISNIAAANSLFS
                     HRLHDRLGEPQYTDSLHSQGSASSMWMRHVGGHERSRAGDGQLNTQANRYVLQLGGDL
                     AQWSSNAQDRWHLGVMAGYANQHSNTQSNRVGYKSDGRISGYSAGLYATWYQNDANKT
                     GAYVDSWALYNWFDNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEGTLNTWY
                     VQPQAQITWMGVKDSDHTRKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDGKQREFQ
                     PYIEANWINNSKVYAVKMNGQTVGREGARNLGEVRTGVEAKVNNNLSLWGNVGVQLGD
                     KGYSDTQGMLGVKYSW"
     gene            5794..6060
                     /gene="hipB"
                     /locus_tag="RHZ76_003724"
     CDS             5794..6060
                     /gene="hipB"
                     /locus_tag="RHZ76_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HDV2814292.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDAKNASPESTEQQNLEW"
     gene            6060..7382
                     /gene="hipA"
                     /locus_tag="RHZ76_003725"
     CDS             6060..7382
                     /gene="hipA"
                     /locus_tag="RHZ76_003725"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HDV2814293.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGTQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK"
     gene            7632..>8503
                     /locus_tag="RHZ76_003726"
     CDS             7632..>8503
                     /locus_tag="RHZ76_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001388361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV2814294.1"
                     /translation="MFILTQLNINQRQRLWALMDTHTRQPLLYPLIYLIDHLALRSSA
                     TQSASLQALKFFYEFWHQKHGVTFCFSFYSSNHNPLIAIDELTAFFHYLENTHLYVPA
                     LTIRSTTQTTPQRCTNIRHVHSVIRFIRYLINTYISPRYIDGTPKELTRLATQLTGRL
                     SIHKAEFRTLTHSRQMNNGTTHKRFRSLTAEMVMAFYQIITPGSISKKNPLNPFPAGE
                     IQLRNFLICRLLLNYGLRVSELLLLECHSIKPNLRGDQFSLIVTTVDDDVSDQRKRLP
                     SLKNVYANRVLALD"
BASE COUNT         2320 a         1839 c         2265 g         2079 t
ORIGIN      
        1 ttcgaaagca ttagcaaatt agccaaattg acaccttcgc acctccaact aacactcaat
       61 acagacctct gacaatttaa taagtttcat ttttaatgtc tgacgactca tctctttcgt
      121 ggctgtattg tttgtatatg gagcgtttat atgaatagaa tctatcgcgt gatatggaat
      181 tgcactctac aggtatttca ggcctgctct gaattaactc gcagggtagg taaaacatca
      241 acggttaatt tgcgtaaatc ctctggactg acaacgaaat tcagtagatt gacgctgggt
      301 gttttgctgg cactaagcgg ttcagtgtct ggtgcgagtc tggaagttga taatggtcag
      361 attaccaata ttgatactga tgttgcttat gatgcctacc tggttggctg gtatggcact
      421 ggagtgctta atattttggc tggcggtaat gccgccttaa ccactattac taccagcgtc
      481 attggcggta atgaggattc ggagggtacc gttaatgttt tgggtggcac ctggcgattg
      541 tatgatagcg gaaataatgc aaggccttta aatgtgggtc aatccggaac ggggacgctg
      601 aatattaaac agaagggtca cgtcgatgga ggctatttaa gattaggtac tcaaactgca
      661 ggcgtcggga cggttaatgt tgagggagag gactcggttt tgacgaccga attattcgaa
      721 ataggtagct atggcacagg ttcattaaat attacggata agggttacgt cacgagttca
      781 atcgtcgcca ttttaggcta tcaggcgggc agtaatggcc aggttgtcgt tgaaaagggt
      841 ggcgagtggc taataaaaaa taatgattcc tcaattgaat ttcaaattgg taatcaagga
      901 actggggagg cgactattcg cgaggggggc tggttacggc tgaaaatacg attatcggtg
      961 gcaatgccac cggtatcgga accctgaatg tgcaggatca agactctgtc atcacggtac
     1021 gcagactcta taatggatat ttcggtaatg gcacagtcaa tatttccaat aatggactga
     1081 ttaataacaa agaatattca ttggtgggcg ttcaggacgg ttcccacggt gtcgtcaacg
     1141 tgaccgataa agggcattgg aatttcctcg gaacgggcga agctttccgc tatatctata
     1201 tcggtgatgc tggcgacggt gaacttaatg tctcgagtga aggcaaagta gattcgggaa
     1261 ttatcactgc ggggatgaaa gaaacaggca caggcaacat tactgttaag gataagaact
     1321 ccgttatcac taatctcgga actaatcttg gttatgacgg ccacggcgaa atgaatatca
     1381 gtaatcaggg gcttgttgtc agcaacggag gaagttcact cggttatgga gaaaccggcg
     1441 tcgggaatgt cagcatcacc acggggggaa tgtgggaggt caataagaat gtctatacca
     1501 ccattggtgt tgcgggcgtc ggaaacctca atattagcga tggcggtaag ttcgtatcgc
     1561 aaaatattac ttttttgggt gataaagcaa gcggtatcgg cacattgaac ctgatggatg
     1621 cgacatcatc gttcgatact gtgggtatca atgtcggtaa ttttggtagc ggcatcgtaa
     1681 atgtcagtaa tggcgccacc cttaattcaa cgggctatgg atttatcgga ggaaatgcct
     1741 ccggtaaggg aatagttaat atttcaacgg atagtctctg gaatttaaag acgtcttcta
     1801 ctaacgccca attgctacaa gtcggtgtat taggcacggg tgaactgaat attaccaccg
     1861 gaggtatagt taaagcgcgt gatacacaga tagctctcaa tgacaaaagt aagggcgacg
     1921 tgagggtgga tgggcagaac tctcttcttg aaacattcaa tatgtacgta gggacatctg
     1981 gtacgggtac gttaaccctg acgaatagcg gcacgctgaa tgtcgaaggt ggagaagttt
     2041 acttaggtgt ttttgaacct gctgtaggaa cgctaaacat tggtgcagct cacggtgagg
     2101 cggcggcaga tgccgggtat ataaccaacg cgacgaaagt agagtttggt tctggcgaag
     2161 gtgtttttgt ctttaatcat actaataaca gtgatgccgg ctaccaggtc gatatgctga
     2221 ttacgggtga cgataaagac ggaaaagtga tccatgatgc aggccatacg gtgttcaatg
     2281 cagggaatac ttatagcggt aaaacgctgg tcaatgacgg cctcctgacc attgcgtctc
     2341 atacggcaga tggggtaacg ggcatgggtt cgagtgaagt caccattgca agccccggta
     2401 cgctcgacat tctcgcatca acgaacagtg caggagatta caagctgacc aatgcgctca
     2461 aaggcgatgg tttgatgcga gtgcagctgt catcctccga caagatattt ggctttaccc
     2521 atgcaacagg gactgaattc gccggtgttg cccaactgaa agacagtacc ttcactctgg
     2581 aacgcgacaa caccgctgcg cttactcacg cgatgttgca gtctgacagt gaaaatacca
     2641 catcggtaaa cgtgggagag caatccattg gtggactggc catgaatggc ggtacgctca
     2701 ttttcgatac ggatattcct gctgcgacgc ttgcagaggg atatatcagc gtcgatacgc
     2761 tggttgtcgg cgcgggtgac tacacctgga aaggccgtaa ctatcaggta aacgggacgg
     2821 gcgacgtgct tatcgacgtg cctaaaccgt ggaatgatcc catggcgaat aaccctctga
     2881 cgacgctcaa tttgctggaa cacgatgata gccatgtcgg cgttcaactg gtgaaggcgc
     2941 aaacggttat tgggtcgggt ggctcattaa cgttacgtga tttacagggc gacgaggtgg
     3001 aagcggacaa aacgttacac attgcgcaaa acggaacggt ggtcgccgag ggtgattatg
     3061 gattccgcct cacgaccgca ccaggtgatg gtttgtacgt taactatggg ctgaaagcgc
     3121 tgaacatcca tggtgggcaa aagctgacgt tagccgaaca tggcggagcc tatggcgcaa
     3181 cggccgatat gtcggcaaaa atcggtggtg aaggggatct ggcaatcaat acggtgcgac
     3241 aggtttcgct ttccaacggt cagaacgact atcagggggc aacctacgtt cagatgggga
     3301 cattacgtac cgatgcggat ggtgcacttg gcaacacccg ggaactgaac atcagcaacg
     3361 cggccatcgt cgatcttaat ggatcgacgc agacggtaga gacattcacc gggcagatgg
     3421 gttcgactgt tttgttcaaa gagggggcgc tgacggtaaa taaaggtggg atcagtcagg
     3481 gtgaactgac aggtggcgga aacctgaatg ttacaggggg aacgctggct atcgaggggc
     3541 ttaatgcacg ctacaatgcg ttaaccagca ttagcccaaa tgcggaagtc agcctcgata
     3601 atacgcaggg gttaggcaga ggaaatattg ccaatgacgg tctgttaacg ctaaaaaacg
     3661 tgactggcga actgcgcaat agcataagcg ggaagggtat cgtgagcgca accgccagga
     3721 cagatgtaga gctggatggc gataatagcc gctttgtggg gcaattcaac attgatacag
     3781 gcagcgcgct tagcgtcaac gagcagaaaa acctgggtga tgcttccgtt atcaataatg
     3841 gcctgctcac catctccact gagcgtagct gggcgatgac gcacagtatc agcggtagcg
     3901 gtgatgtgac aaaactgggt accgggatcc tgactcttaa caacgattcc gcggcgtatc
     3961 agggtacgac ggatatcgtg ggggggggaa attgctttcg gttccgactc tgccattaat
     4021 atggcaagtc aacacattaa tatccataac agcggtgtga tgtcgggaaa tgtcaccact
     4081 gcaggtgata tgaacgttat gcctgggggg gcactgcgtg tcgctaaaac cactatcagc
     4141 ggcaacctgg agaatggcgg cacggttcaa atgaatagcg aaggggggaa accggggaat
     4201 gtactgaccg ttaacggcaa ctataccgga aacaatggcc tgatgacgtt caacgcgacg
     4261 ctgggcggcg ataattcgcc caccgataag atgaacgtga aaggcgatac ccaagggaac
     4321 actcgcgttc gggttgataa cattggcggc gtcggtgcac aaacggtcaa cggtattgaa
     4381 ctcattgagg ttggcggtaa ttctgcaggt aacttcgcgc tgaccaccgg aactgtcgaa
     4441 gctggggctt acgtctacac gctggctaaa gggaagggga atgacgagaa aaactggtat
     4501 ctgaccagta aatgggacgg cgtaacgcca gcggatacac ccgatcccat caataatccc
     4561 cctgttgtgg atccggaagg cccatcagtt tatcgcccgg aggccggaag ctatatcagc
     4621 aacattgccg cagccaactc gctgtttagc catcgtttac acgaccgtct gggtgagccg
     4681 cagtatacag attcactgca ttctcagggg tcggcaagca gtatgtggat gcgtcatgtc
     4741 ggagggcacg aacgttcaag ggccggtgac ggtcagctaa atactcaggc taaccgctat
     4801 gtattgcagc taggcggcga tttggcgcag tggagtagca acgcgcagga tcgctggcat
     4861 cttggcgtga tggcaggcta cgccaatcag cacagtaata ctcagagtaa tcgtgtgggt
     4921 tataaatcgg atgggcgcat cagcggttac agcgctgggc tgtacgcgac ctggtatcag
     4981 aacgatgcga ataagaccgg cgcttatgtt gacagctggg cgctgtataa ctggtttgat
     5041 aacagcgtca gttccgataa ccgttctgct gacgactatg attctcgcgg tgtgacggcc
     5101 tctgttgagg gtgggtatac ctttgaagcg ggaacattta gcggcagcga agggacgctg
     5161 aatacctggt acgtccagcc acaggcgcaa atcacctgga tgggtgtgaa agattccgac
     5221 catacccgga aagacggaac gcgcattgaa acggaaggcg acggaaatgt gcaaacgcga
     5281 cttggggtga aaacctacct gaatagccat caccagcgtg acgatggtaa acagcgtgag
     5341 ttccagcctt acattgaagc gaactggatc aacaatagca aagtctacgc cgtgaagatg
     5401 aatggtcaaa ccgtaggccg tgaaggtgcg cgtaatctcg gtgaagtacg taccggggtt
     5461 gaggcgaaag taaataacaa ccttagcctg tgggggaatg tcggtgtgca actaggtgat
     5521 aaaggctata gcgatactca gggcatgctg ggagtgaaat atagctggta aaccgtataa
     5581 gccgcatgtc gagatggcat gcggcttaat attgccgact tcaaacggcg catcaacgcc
     5641 ttatttaaat cctccttttt atccgcgatc gcggatatcg cagcgtttat cccgtagagc
     5701 ggataagatg tgtttccaga ttgacttatc ctcactaaag gataaaactt ataatatccc
     5761 cttaagcgga taaacttgct gtggacgtat gacatgatga gctttcagaa gatctatagc
     5821 ccaacgcaat tggcgaatgc aatgaaactg gttcgccagc aaaatggctg gacgcagagc
     5881 gagctggcga aaaaaattgg tattaagcag gcgacgattt ccaatttcga aaacaaccct
     5941 gacaatacca cgctcacgac attttttaag attttacagt cgcttgaact ctcaatgacg
     6001 ctatgcgacg cgaaaaatgc ctcgccagaa tcaacagaac agcaaaatct ggagtggtaa
     6061 tgcctaaact tgtcacttgg atgaacaacc agcgggtagg cgagttaacg aagttagcca
     6121 acggcgcgca cacctttaag tatgcaccgg agtggttagc aagccgttat gccagaccgt
     6181 tgtcactttc gctgccattg cagaggggga atatcacctc tgatgccgta tttaacttct
     6241 tcgataacct gttacccgat agcccgattg tacgtgaccg gatcgttaaa cgttatcatg
     6301 ccaaatccag acaaccgttt gatttattgt cagaaatagg gcgagacagc gttggtgccg
     6361 tgacgttaat acccgaagac gaaaccgtaa cgcatccgat aatggcatgg gaaaagctta
     6421 ctgaagccag acttgaagaa gtattaacgg cttataaagc agatatcccg ctaggcatga
     6481 ttagagaaga aaatgacttt cgcatctcgg ttgctggcac acaggagaag acagcactgc
     6541 tcagaatagg caatgactgg tgcattccga aaggaataac gccgacgacg cacatcatta
     6601 aattaccgat tggcgaaatc aggcagccca atgcgacgct cgatctcagc caaagcgttg
     6661 ataatgagta ttactgtctg ctgctggcga aagaacttgg gttgaatgtt ccggacgcag
     6721 aaatcattaa agcgggaaat gtgcgcgcgt tagcggtcga acgttttgac aggcgttgga
     6781 atgctgagcg aacggtttta cttcgcttgc cacaggagga tatgtgtcag acattcggtt
     6841 taccttcatc ggtgaaatat gaatcagatg gaggcccagg catcgcgcgg atcatggctt
     6901 ttttgatggg gtccagcgag gcgctgaaag atcgctatga ttttatgaaa ttccaggtct
     6961 tccagtggtt gattggcgca acggacggtc atgcaaaaaa cttctccgta tttattcagg
     7021 ctggcggcag ttatcgactc acgccatttt acgacatcat ttcagcattt ccggtccttg
     7081 gcggtacggg aatacacatc agcgatctca aactggcaat ggggcttaac gcatccaaag
     7141 gcaaaaaaac ggcaatcgat aaaatttatc cgcgacattt tttggcgaca gcaaaggtgc
     7201 tgagattccc ggaagtgcag atgcatgaaa tcctgagtga ctttgccaga atgattccag
     7261 cagcactgga taacgtgaag acttcattac cgacagattt tccggagaac gtggtgacgg
     7321 cagttgaaag caatgtgttg aggttgcatg gacggttaag ccgagaatac ggtagtaagt
     7381 gagatatggg gtttggtcat tgttaatgag catgacaatc atgaccgcca acgactggat
     7441 tcttccttgg tttatattgg tgcgcataat gtatattatg ttaaatttag tccgtcggtc
     7501 atttagttca taaccggcca caggatacga ttaatcatac acttacctac ccctctatct
     7561 tcttcttgca ggacgttatg tttgtcggtt actttgttca tatccacacc attgataagg
     7621 ttaagtgccg cgtgttcata cttacacaac tcaatatcaa ccaacggcag cgtttatggg
     7681 cactgatgga tactcacacg cgccagccgc ttttatatcc gttaatatat cttatcgacc
     7741 atcttgcttt gcgttcatca gccacgcaat ccgcttcact tcaagcgctc aagtttttct
     7801 atgaattctg gcaccagaaa catggtgtga ctttttgctt cagtttctat tcctcgaacc
     7861 acaatccatt gattgccatt gatgaactta cagccttttt tcattatctg gaaaatacgc
     7921 atttatatgt accagcgtta accataaggt ctactacgca gaccacgcct caacgttgta
     7981 caaatatcag gcatgtacat tcggttattc gttttatcag atatttgatt aacacgtata
     8041 tctcgccgag atatatagat ggcacgccta aagagcttac acgacttgcc acgcagttaa
     8101 cgggacgttt atcgatacat aaggcagaat tccgtactct tacccattct cgtcagatga
     8161 acaatggcac gacccataaa cgctttcgaa gtctgactgc tgagatggtc atggcttttt
     8221 accaaatcat tacacccggt tcaatttcaa agaaaaaccc actcaatccc ttccccgctg
     8281 gtgaaatcca gctacgtaat tttctgattt gcagattgct tttaaattac ggattgcgtg
     8341 tcagtgaatt actgcttttg gagtgtcatt cgatcaaacc aaatctccga ggggatcagt
     8401 tcagcctgat tgtgaccaca gtagatgacg acgtttcgga tcaacgaaaa agattgcctt
     8461 cattaaaaaa tgtttatgca aaccgggtgc tggcattaga taa
//
LOCUS       DAPHBJ010000185         8401 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.187, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000185 DAPHBJ010000000
VERSION     DAPHBJ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.187"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(171..377)
                     /locus_tag="RHZ76_003727"
     CDS             complement(171..377)
                     /locus_tag="RHZ76_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HDV2814295.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            690..779
                     /gene="kdpF"
                     /locus_tag="RHZ76_003728"
     CDS             690..779
                     /gene="kdpF"
                     /locus_tag="RHZ76_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDV2814296.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            779..2452
                     /gene="kdpA"
                     /locus_tag="RHZ76_003729"
     CDS             779..2452
                     /gene="kdpA"
                     /locus_tag="RHZ76_003729"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDV2814297.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2475..4523
                     /gene="kdpB"
                     /locus_tag="RHZ76_003730"
     CDS             2475..4523
                     /gene="kdpB"
                     /locus_tag="RHZ76_003730"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDV2814298.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWITVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAEDKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4532..5104
                     /gene="kdpC"
                     /locus_tag="RHZ76_003731"
     CDS             4532..5104
                     /gene="kdpC"
                     /locus_tag="RHZ76_003731"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDV2814299.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5097..7781
                     /gene="kdpD"
                     /locus_tag="RHZ76_003732"
     CDS             5097..7781
                     /gene="kdpD"
                     /locus_tag="RHZ76_003732"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HDV2814300.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7778..>8401
                     /gene="kdpE"
                     /locus_tag="RHZ76_003733"
     CDS             7778..>8401
                     /gene="kdpE"
                     /locus_tag="RHZ76_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HDV2814301.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATTAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPA"
BASE COUNT         1765 a         2187 c         2443 g         2006 t
ORIGIN      
        1 aagtgagata tctgtcagat aagaacaatt aggaaaatat ttatatatat ttcatgaata
       61 tatgtttcga ttggtataac gatgtctaaa tattcctctg aacatcttcc tgatacaaaa
      121 aaccgccagc gtttccgctg gcggttttca atgtattcaa aactcagcaa tcaataaaaa
      181 tcaccagttg ccttttcggc ctgatccatc cacaccggtt tatcgctggt tttcgaccag
      241 acgcgatgca ggtagctgta aaagcgggcg cggtctttcc agaaaagcat gaaaggcagc
      301 gccagaacgc ccgctgcaac cgcataagta cggcgtaaaa agataagcca tgcaggatat
      361 tctctgtaga gttccatact tttctccccc ttttgtatct acccggtgaa tggcaccgga
      421 aaaatgaatt tgtttatctg atgaaaatag taccgccttt tgtgtaattt tactactcat
      481 ccgaccactt atttttgctt attgatggtt tatttacatt catcctgtaa ttaagttaca
      541 caaaagttaa attaatacta aacattagtt aaatcatggc ttttgccatt tttatacttt
      601 ttttacaccc cgcccgcaga tttttgcgaa atctttgcag ccagaattct acccttccgg
      661 tatcactttt aggccactgg aggtgcactg tgagtgcagg cgtgataacc ggcgtattgc
      721 tggtgttttt attactgggt tatctggttt atgccctgat caatgcggag gcgttctgat
      781 ggctgcgcaa gggttcttac tgatcgccac gtttttactg gtgttaatgg tgctggcgcg
      841 tcctttaggc agcgggctgg cgcggctgat taatgacatt cctcttcccg gtacaacggg
      901 cgttgagcgc gtactttttc gcgcacttgg cgtctctgac cgtgagatga actggaagca
      961 atatctctgt gccattctcg gcctgaacat gctggggctg gcggtgctgt tttttatgtt
     1021 gctcggtcag cactatctgc cgcttaatcc acagcagttg ccggggctgt cgtgggatct
     1081 ggcgctgaat accgccgtca gctttgtcac gaataccaac tggcaatctt acagcggtga
     1141 aaccacgttg agctatttca gccagatggc tggcttaacg gtgcaaaact ttctttctgc
     1201 cgccagcggg attgcggtga tttttgccct catccgtgcg tttacccgcc agagcatgag
     1261 cacactcggg aatgcctggg tcgatctgct acgcatcacg ttatgggtgc tcgtccctgt
     1321 ggcgttgttg atcgcactgt tttttattca acaaggtgcg ctgcaaaact ttcagcctta
     1381 tcaggctgtg aataccgttg aaggagcgca acagctgtta cccatggggc ctgtagcttc
     1441 tcaggaagcg atcaagatgc tcggtactaa cggcggtggc ttctttaatg ccaactcgtc
     1501 gcatccgttt gaaaacccaa ccgcactgac caacttcgtg cagatgctgg caatcttctt
     1561 gatcccaacg gcgctgtgct ttgcctttgg tgaagtgacg ggcgatcgcc gccaggggcg
     1621 catgttgctg tgggcgatgt cagtgatttt tgtcatctgc gtaggcgtgg tgatgtgggc
     1681 agaagttcag ggtaatcctc atctgctggc gctcggcgca gacagcagca tcaatatgga
     1741 aggtaaagag agccgtttcg gcgtgctggt cagtagcctg tttgcggtcg tgactacggc
     1801 ggcttcctgt ggcgcggtga ttgcgatgca tgattcgttt accgctctcg gtggcatggt
     1861 gccgatgtgg ctgatgcaaa ttggtgaagt ggtgttcggc ggtgtcggtt ctggtcttta
     1921 cggcatgatg ctgtttgtcc tgctggcggt gtttattgcc gggctgatga ttggtcgtac
     1981 accggaatat ctgggtaaaa aaatcgacgt acgtgagatg aaactgactg cactggcgat
     2041 tctggtcacc ccgacgctgg tgctgatggg cgcggcgctg gcaatgatga ccgacgccgg
     2101 acgtagcgcc atgctcaacc ctggcccgca tggttttagc gaagtgctgt acgccgtgtc
     2161 atccgccgct aacaacaacg gcagcgcctt tgccggatta agcgccaact ctccgttctg
     2221 gaactgttta ctggcgttct gcatgtttgt cggtcgcttc ggggtgatta tcccggtgat
     2281 ggcaattgcc ggttcgctgg tgagtaaaaa gagccaaccc gccagctccg gcacgctgcc
     2341 aacgcacggc ccgctgtttg ttggcctgtt aatcggcacc gtgttgctgg ttggcgcact
     2401 gacctttatc cctgccctgg cgcttggtcc ggtggcggaa tatctctcct gatgatattg
     2461 agtgagcact gaatatgagt cgtaaacaac tggcgctatt cgaaccaaca cttgtcgttc
     2521 aggcgctgaa agaagcggtg aaaaaattaa acccgcaggc gcaatggcgc aatccggtga
     2581 tgtttatcgt ctggatcggc agtctgctga ccacctgtat tagcatcgcg atggcaagcg
     2641 gtgcgatgcc tggcaatgcg ctgtttagcg cggccattag cggctggctg tggatcaccg
     2701 tactgttcgc taatttcgcc gaggcgctgg cagaaggccg cagtaaagcg caggccaaca
     2761 gtctgaaagg ggtgaaaaaa actgcctttg cccgcaagct gcgtgagccg aaatatggcg
     2821 ctgcggagga caaagttccc gccgatcaac tgcgtaaagg cgatatcgta ctggtagaag
     2881 ctggcgatat tatcccctgc gatggtgaag tcattgaagg gggcgcatcg gtcgatgaaa
     2941 gcgccatcac cggggaatcg gcaccggtga tccgtgaatc cggcggcgat tttgcctccg
     3001 tcaccggcgg cacgcgtatt ctttctgact ggctggtgat tgagtgtagc gttaaccccg
     3061 gcgagacatt tctggatcgg atgatcgcga tggtggaagg cgcacagcga cgtaaaacgc
     3121 cgaacgagat tgccctgacc atcctgctga ttgccctgac tatcgtcttt ttactggcaa
     3181 ccgccacgct gtggccgttt tccgcgtggg gcggtaatgc agtcagcgta acggtactgg
     3241 tggcgctgct ggtctgtctg atcccaacca ctattggcgg cctgttgtca gcgatcggcg
     3301 tcgccgggat gagccggatg ctaggcgcga atgtgattgc caccagcgga cgtgcagttg
     3361 aagcggcagg tgacgttgac gttctgctac tggataaaac cggcaccatc acactcggta
     3421 accgtcaggc gtcggagttt atccctgcgc agggcgtgga tgaaaaaacg ctggctgacg
     3481 ccgcacaact ggcttcgctg gctgatgaaa cgccggaagg ccgcagtatt gtgatcctcg
     3541 ccaagcaacg ttttaacctg cgcgagcgcg atgtgcagtc gctccatgcc acctttgtac
     3601 cgtttactgc gcaaagccgg atgagcggga tcaacatcga caaccgcatg atccgtaaag
     3661 gttctgtcga tgccattcgc cgccatgttg aggctaacgg cggtcacttc cctgccgatg
     3721 ttgatcaaaa agtcgatcag gttgcgcgtc agggggccac gccgctggtg gtggtggaag
     3781 gttcccgtgt gctgggcgtt attgcgctga aagatatcgt caaaggcggt attaaagagc
     3841 gcttcgccca gctgcgcaaa atgggcatta aaacggtgat gattaccggc gataaccgtc
     3901 tgactgccgc cgccattgct gcggaagcgg gcgtcgatga ttttctcgcc gaagcgacac
     3961 cggaggccaa gctggcattg attcgtcagt atcaggcgga aggccgtttg gtggcgatga
     4021 ccggcgacgg caccaacgat gctccggcgc tggcgcaggc agatgtcgcg gtggcgatga
     4081 actccggcac ccaggcggcg aaagaggcgg gcaatatggt cgatctcgac tctaacccga
     4141 ccaagttgat cgaggtggtg catattggca aacagatgct gatgacccgt ggctcgctga
     4201 ccaccttcag cattgccaac gatgtggcga aatacttcgc cattattccg gcggcgttcg
     4261 cggcaacgta tccgcagtta aatgcgctga acatcatgcg ccttcattcg cccgactccg
     4321 ccattctcag tgcggtcatt ttcaacgcct tgattatcgt ctttttgatt cctctggcgt
     4381 taaaaggcgt gagttataaa ccgcttaccg cttctgccat gttgcgccgt aacttatgga
     4441 tttacggtct gggtgggctg ctggtgccgt ttattggtat caaagtcatt gatttactgc
     4501 tgaccgtttg cggtctggtg tgaggtttac catgcgtggc ttacgtccgg cattatcgac
     4561 atttatcttt ctgttattga ttactggcgg cgtttacccg ctgctgacca ccgtgctggg
     4621 gcaatggtgg tttccctggc aggccaacgg ttcgttgatt cgtgaaggtg atacggtgcg
     4681 gggttcggca ttaatcgggc agaattttac cggcaacggc tattttcagg gtcgcccgtc
     4741 ggcaacggca gaaatgccct ataatccaca ggcttctggc gggagcaatc tggcggtcag
     4801 taaccctgag ctggataaac aaatagccgc acgcgttgct gcattacggg ccgctaaccc
     4861 ggatgccagt acgaacgttc cggttgaact ggtgacggca tcggcaagcg ggctggacaa
     4921 taatatcacc ccgcaagcgg cggtctggca aatcccacgc gtggcgaaag cgcgtaatct
     4981 cagcgttgaa cagctcacgc aactgatcgc aaaatacagc caacaaccgc tggtgaaata
     5041 tatcggccag ccggtcgtca acattgttga actcaatctg gcgctggata aacttgatga
     5101 ataacgaacc cttacgtccc gaccccgatc gtctgctgga acaaactgcc gcgccgcatc
     5161 gggggaagct gaaagttttc ttcggtgcct gtgcaggcgt cgggaagacc tgggcgatgc
     5221 tggcagaagc ccagcgactg cgggcgcaag ggctggatat tgtggttggc gtggtagaaa
     5281 cccatgggcg gaaagatacc gccgccatgc ttgaagggct ggctgttctg ccgcccaaac
     5341 gccaggcgta ccgtgggcgg catatcagcg agtttgatct cgatgccgcc ctcgcccgcc
     5401 gtccggcgct gatcttaatg gacgaactgg cgcacagtaa tgcgccaggt tctcgtcatc
     5461 ctaaacgctg gcaggatatc gaagaactgc tggaagctgg cattgatgtt ttcacaaccg
     5521 tcaacgttca gcatctggaa agtctgaatg atgtggtcag cggcgtcacc ggaattcagg
     5581 tacgggaaac cgtgcccgat ccttttttcg atgccgccga cgacgtagtg ctggtggact
     5641 tgcccccgga cgatctgcgc cagcggctga aagaaggcaa agtctatatt gccgggcagg
     5701 cggagcgcgc cattgaacat tttttccgca aaggcaatct gatcgccctg cgtgaactgg
     5761 cactgcgccg tactgccgat cgcgtagatg agcaaatgcg cgcctggcgg gggcatcctg
     5821 gtgaagagaa agtgtggcac acgcgcgacg cgatcctttt atgtatcggg cataacaccg
     5881 gcagtgagaa actggtccgc gcggcggcgc ggttggcctc gcgcctgggt agcgtctggc
     5941 acgcggtgta tgttgaaacc cctgccctgc atcgcttacc ggaaaaaaaa cgtcgggcaa
     6001 ttctcagcgc cttacgtctg gcgcaggaac tgggcgcgga gacggcaaca ctttctgatc
     6061 cagcagaaga gaaagcggta gtgcgttatg cccgtgaaca taatctcggc aagattattc
     6121 tcggtcgccc agcctcgcgc cgctggtggc gtcgggaaac gtttgctgac cgactggcgc
     6181 gcatcgcccc cgatctcgat caggtgctgg tcgcgcttga tgaaccaccc gcccgcacga
     6241 ttaacaacgc gccggatagc cgctctttta aagacaagtg gcgggtacag attcagggat
     6301 gcgtggttgc cgccgcgtta tgcgccgtta tcaccttaat tgccatgcag tggctgatgg
     6361 cgtttgatgc cgccaacctg gtgatgctgt atctgcttgg cgtggtggta gtggcgctat
     6421 tttatggacg ctggccttcg gtggttgcta ccgtcattaa tgtggtgagt ttcgatctct
     6481 tttttatcgc cccacgcggc acgctcgccg tctctgatgt gcaatatctg ctgaccttcg
     6541 cggtgatgtt aaccgtcggg ctggtgatcg ggaaccttac tgctggcgtg cgttatcagg
     6601 cgcgggtagc ccgctaccgc gagcaacgca cacggcactt atatgaaatg tcgaaagctc
     6661 tggcggtggg ccgcagtccg caggacatcg ccgccaccag cgaacaattt attgcctcca
     6721 cgtttcatgc ccgcagtcag gtgttgttgc ccgatgacaa cggtaaattg cagccgttaa
     6781 cacatccgca aggaatgacg ccatgggacg atgccatcgc gcagtggagt tttgataaag
     6841 gcctgcctgc gggtgcgggc accgacacgt tacccggcgt gccgtaccag attttgccgc
     6901 taaaaagcgg cgagaaaacc tacggactgg tggtggtgga accggggaat ctgcgccagt
     6961 tgatgatccc ggagcagcag cgcctgctgg agacatttac gctgttagtc gccaatgccc
     7021 ttgagcggct gacactgacc gccagcgaag aacaggcgcg gatggcaagc gaacgtgaac
     7081 agatccgcaa cgccctgctg gcggcgcttt cgcatgattt acgcacgccg cttacggtgc
     7141 tgtttggtca ggcagaaatc ttaacgctcg atctggcaag cgaaggatcg ccccacgccc
     7201 gccaggccag cgagattcgt cagcatgtgc tgaacactac ccgactggtg aataatctac
     7261 tggatatggc gcgaattcag tccggcggct ttaatttgaa gaaagagtgg ttaacgctgg
     7321 aagaagtagt cggcagcgcg ctgcaaatgc tggaaccggg tttatcgtcg cccatcaatc
     7381 tttctctgcc agaaccgctg accttaatcc acgttgacgg gccactcttt gaacgggtgc
     7441 tgattaatct gctggagaac gcggtgaaat atgcgggtgc gcaggccgaa attggtatcg
     7501 atgcccacgt tgagggcgaa aatctacaac tggatgtctg ggataacggc cccggtcttc
     7561 cgccaggcca ggagcagacg atatttgata agtttgctcg cgggaataaa gagtcggcag
     7621 taccgggggt agggcttgga ctggcaattt gtcgggcgat agtggatgta cacgggggca
     7681 ctattaccgc gttcaaccga ccggaaggtg gtgcctgttt tcgtgttaca cttccccagc
     7741 aaactgcccc tgaacttgaa gaatttcatg aggatatgtg acaaacgttc tgattgttga
     7801 agatgaacag gctattcgtc gctttctgcg cacggcgctg gagggcgacg ggatgcgcgt
     7861 ctttgaggcc gaaacgctgc aacgcggctt gctggaagcg gcaacccgta agccagattt
     7921 gattattctc gatctcggcc tgcccgatgg tgatgggatt gagtttatcc gcgacctgcg
     7981 ccagtggagc gcggtgccgg tgattgtgct ttccgcacgc agcgaagaga gcgacaaaat
     8041 cgccgcgctg gatgccggag cggatgatta tctgagtaag ccgtttggca ttggcgaatt
     8101 gcaggcccgt ctgcgcgtcg cattacgccg ccactctgcc accaccgcgc ccgatccgct
     8161 ggtaaaattt tccgatgtta ccgtcgattt agccgcccgc gtgattcacc ggggtgagga
     8221 agaggtgcat ctcacaccaa ttgagttccg cctgctggcg gtgctgctca acaatgccgg
     8281 aaaagtactc acccagcgcc agctccttaa ccaggtgtgg gggccaaacg cggtcgaaca
     8341 cagtcactat ttgcgtattt atatgggaca tctgcgacaa aaactggaac aggatcccgc
     8401 c
//
LOCUS       DAPHBJ010000186         8395 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.188, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000186 DAPHBJ010000000
VERSION     DAPHBJ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.188"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(138..4025)
                     /gene="purL"
                     /locus_tag="RHZ76_003734"
                     /gene_synonym="purI"
     CDS             complement(138..4025)
                     /gene="purL"
                     /locus_tag="RHZ76_003734"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDV2814302.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELREILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRNEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            4283..5839
                     /gene="mltF"
                     /locus_tag="RHZ76_003735"
     CDS             4283..5839
                     /gene="mltF"
                     /locus_tag="RHZ76_003735"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HDV2814303.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(5836..6372)
                     /gene="tadA"
                     /locus_tag="RHZ76_003736"
     CDS             complement(5836..6372)
                     /gene="tadA"
                     /locus_tag="RHZ76_003736"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDV2814304.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(6397..7032)
                     /gene="yfhb"
                     /locus_tag="RHZ76_003737"
     CDS             complement(6397..7032)
                     /gene="yfhb"
                     /locus_tag="RHZ76_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HDV2814305.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            7241..8089
                     /locus_tag="RHZ76_003738"
     CDS             7241..8089
                     /locus_tag="RHZ76_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDV2814306.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            8145..>8395
                     /gene="yfhL"
                     /locus_tag="RHZ76_003739"
     CDS             8145..>8395
                     /gene="yfhL"
                     /locus_tag="RHZ76_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HDV2814307.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHA"
BASE COUNT         2028 a         2310 c         2168 g         1889 t
ORIGIN      
        1 gtgtcgccag aaagcgacac ggcaaaccac ccattgtcgt gatttacaga cacaaaaaag
       61 ctcccggagt tgggagctta tgatagtggt tggtgcttaa tgccgcatcc gggctgcaaa
      121 accaatgggc tgacgactta ccccaactgc ttacgcgcat tgcggaaaat acgcatccat
      181 gggccatcct cgccccagtt ttccggatgc caggagttgc tgacagtacg gaaaacacgt
      241 tccgggtgcg gcatcataat ggtgactcga ccactttcag tcgtgactgc cgtaataccg
      301 ttcggtgaac cgttcgggtt agccgggtag gtttcagtga ctttgccgaa gttatcgaca
      361 tagcgcagtg ccaccagccc tttgctttcc agtgccgcca gatgcgccgc atcacgcact
      421 tccacgcgcc cttcaccatg agagacagca atcggcattt gcgagcccac catcccctgc
      481 aacagcagcg acgggctttg ggtcacttca accaggctga aacgcgcttc aaagcgatcg
      541 gaggtattgc gcacaaaacg tggccacaac tcactacctg ggatcagttc acgcagatta
      601 gacatcatct ggcaaccgtt acataccccc agcgccagcg tttgcggacg gtggaagaag
      661 gttgcaaact cattgcgtac acggtcattg aacaggattg acttcgccca accttcaccg
      721 gcacccagca catcaccgta ggagaaacca ccgcacgcga ccagggcgtg gaaatcttcc
      781 aggcccgtgc gtccggtcag caggtcactc atatgcacgt cgatagcatc aaagcctgca
      841 cggtggaaag ctgccgccat ttcaacatgc gagttcacgc cctgctcacg cagtacggca
      901 actttcggac gtgcgccagt ggcaatatac ggtgctgcca catcttcgtt gatatcgaac
      961 gacagtttta cattcaggcc cggatcggcg tcgttagatt tcgcctgatg ctcctgatcg
     1021 gcacactccg ggttgtcacg caggcgctgc atctgccagg tagtttctgc ccaccagaca
     1081 cgcaacgtgg tgcggctttc gctgaataca gtctgcccgt tggcggtaat cacaaaacgg
     1141 tcaccggaaa ccgcctgccc tacataatgg acacaatcag caagcccatg ctgtgccagt
     1201 acggactcga ccgcttcacg gtcagcggca cgaacctgaa tcaccgcacc cagttcttcg
     1261 ttaaacaacg ccgccaggcg atcgtcaccc agagtggcga tatccgcgtc aatgccacaa
     1321 tgaccagcaa aggccatttc cgccagcgtt accagcaggc cgccatcaga gcggtcgtga
     1381 tacgccagca gcttacgctg tgcaaccagc gcctgaatcg cgtcatagaa gcctttcagt
     1441 tgcgcgacat cgcgtacatc tgccggtttg tcgccaagct gacgataaac ctgcgccagc
     1501 gccgttgcgc ccagcgcgtt attgcctttg cccaaatcaa tcagcagcag tgcgttatct
     1561 tcggtagaaa gctgcggcgt gatggtgtga cgtacatctt ccacgcgggc aaatgcagaa
     1621 atcaccagcg acagcggcga cgtcatttcg cgctcttcgt taccttcctg ccagcgggtt
     1681 ttcatcgaca tggagtcttt acccaccggg atcgtcaggc ccagcgccgg acaaagctct
     1741 tcgcccacgg ctttaacggc ttcatacagg cccgcatctt caccagggtg gcctgccgcc
     1801 gccatccagt tggcggaaag tttaatgcgt ttgatatcgc caatctgtgt tgcggcaata
     1861 ttggttaacg cttcaccgac cgccagacgg gcagaggcgg cgaaatccag cagcgcaacc
     1921 ggcgcacgct cgccaatcgc catcgcttca ccgtagtagc tgtcgaggct ggcggtagtg
     1981 accgcgcagt tagcgaccgg cacctgccac ggacccacca tctgatcgcg cgctaccatg
     2041 ccagttacgc tgcggtcacc gatggtcacc aggaaagttt tctccgccac ggtcggcaga
     2101 tgcagtacgc gtttcaccgc gtctgcaatg gtgatccctt cacggaccag cgcatcgccc
     2161 ttcgctttca gcgtttgtac atcgcgggtc atcttcggcg ttttaccaag caggacgtcc
     2221 agcggcagat cgatcggctg attatcaaaa tgacgatcgt gcagagaaag atgcagttct
     2281 tcggtcgctt caccaatcac cgcgtagggt gcgcgctcac gcttacacag ttcgtcaaac
     2341 agcggtaatt gatcggcagc aaccgccagc acgtagcgtt cctgggattc gttacaccag
     2401 atttccagcg ggctcatgcc cggttcgtcg cttagaatct cgcgcagttc aaatttaccg
     2461 ccgcgcccgc cgtcgctcac cagttccggc atggcgttag aaagaccgcc agcgccaacg
     2521 tcgtggataa acaggattgg gttggcatca ccaagctgcc agcaacggtc gatcacttcc
     2581 tggcagcgac gctccatctc cgggttgtcg cgctgtacgg aagcaaagtc gaggtcggca
     2641 tcagactgac cagacgccat agaagacgct gcaccaccgc caagaccgat gttcattgcc
     2701 gggccgccga gaacgaccag cttcgcaccg acgttgatct cgcctttttg tacgtgatcg
     2761 gcgcgaatgt tgccgatccc gcccgccagc atgatcggtt tgtgataacc gcgcagctct
     2821 tcgccgttgt ggctgttcac tttttcttca taagtacgga agtagccgtt cagtgccgga
     2881 cgaccaaatt cgttgttaaa cgccgcgccg cccagcgggc cttcggtcat gatgtccagc
     2941 gcggtgacaa tgcgctcagg cttaccgaaa tcttcttccc acggctgttc gaagccagga
     3001 attcgcaggt tggaaacgga gaaaccaacc agacccgctt tcggctttgc gccgcgcccg
     3061 gtggcacctt catcgcggat ttcaccgccg gaaccggtcg ccgcgcccgg ccacggagaa
     3121 atcgccgtcg ggtggttgtg agtttcgact ttcatcagaa tatgcgccgg ttcctgatgg
     3181 aaatcgtagc ggcccgtttc gtggtcagca aagtagcggc ccacttcaga accttccatt
     3241 acggcggcgt tatctttata agcagagaga acgtgatctg gcgtggtttc gaaagtattt
     3301 ttgatcatct tgaacagcga tttcggctgc tgttcaccat cgataaccca gtcggcgtta
     3361 aaaattttgt ggcggcagtg ctcggagttc gcctgggcaa acatatacag ttcgatgtcg
     3421 ttcgggttac gaccaagctt tgtgaaagca tcctgcaaat agtcaatttc atcttccgcc
     3481 agagccaggc caagacgcag gttagcgtcg attagcgcct gacggccctg ccccagcaaa
     3541 tcaacgctgg tgaccggagt cggttgatgg tgtgcaaaca gctgctctgc atcatctaaa
     3601 gcaaaaaaga ccgtttccat catgcggtcg tgcagttcag cggtaacctg ctgccattgt
     3661 tcattggtca gcgtaccggc ttctatatag taagcaacgc cgcgctcaag gcggtttacc
     3721 tgttgtagcc cgcagttatg ggcaatatcg gtcgctttcg aagaccaggg agagatggtg
     3781 ccaggacgcg gggtcaccag caggagtttg ccttgcgggg cgtggctggc gagtgccgga
     3841 ccatatttca gcaggcgttc aagttgtgcg tgctcatcat cgtttaacgg cgcattgagg
     3901 tcagcaaaat ggacatactc ggcgtaaata ttgtgaaccg ggagcctggc agcctgaaaa
     3961 cgtgccagca gtttgttgat tcggaatgcc gacagtgcag gcgaaccacg cagaatttcc
     4021 atcataagtc tctcgtcttc taagctttcg gtgtacccaa gggggggaaa cgggcgtcat
     4081 tataaagaat ctgatgcgct gacgaaaccg tttgcgtgga aataaaatca ccatcgtgaa
     4141 ttagcaacgc gtgccgccaa tggatgtaat aagttgccat ctggcgcagg tttacgcaaa
     4201 atgccgctca tttatgagta aacctttcac tattattacg ttttttcaag ctgggacgcg
     4261 cacgacacag agaattaact aattgaaaaa attaaagatt aattatctgt tcatcggcat
     4321 tctggcactg ctgctcgcgg tcgctctctg gccatccatt ccctggtttg gtaaagccga
     4381 caaccgtatc gccgccattc aagcgcgggg agagttgcgt gtgagcacca ttcatactcc
     4441 cctgacttat aacgaaatca acgggaaacc ttttggcctg gattacgaac tggcgaaaca
     4501 gtttgccgat tacctcggcg taaaactgaa agtgaccgtg cggcagaata tcagccagct
     4561 gtttgacgac cttgataatg gtaacgccga cctgctggcg gcaggacttg tctataacag
     4621 tgagcgggta aaaaattatc agcctggccc tacctattat tccgtgtcac aacaactggt
     4681 ttataaagtg ggtcagtatc gcccacgtac gctgggcaac ctgacggcgg agcaactcac
     4741 cgttgcaccg ggtcatgtgg tggttaacga tctccagacc ctgaaagaaa caaaattccc
     4801 ggaattaagc tggaaggtag acgacaaaaa aggctctgcg gaattaatgg aagatgtcat
     4861 cgaaggaaaa ctcgattaca ccattgctga ttctgtcgcc atcagcctgt ttcagcgcgt
     4921 tcacccggag ctcgccgtag cgctcgatat caccgatgaa caaccggtga cttggtttag
     4981 cccgttagat ggcgataata ccctttccgc cgccctgctc gacttcttca acgaaatgaa
     5041 tgaagacggt acgctggcac gcattgaaga gaaatacctg gggcatggcg atgattttga
     5101 ttacgtcgat acgcgcacat ttttacgcgc cgtcgatgcg gtactgccgc agttaaagcc
     5161 cctgtttgag aaatacgccg aagaaattga ctggcgtttg ctggccgcta ttgcttatca
     5221 ggaatcgcac tgggatgcac aggccacttc accgacgggt gtgcgcggca tgatgatgtt
     5281 aaccaaaaat accgcgcaaa gcctcggcat tacggatcgt accgatgccg aacagagcat
     5341 tagcggtggc gtgcgttatt tgcaggatat gatgagtaaa gtgccggaaa gtgtgccgga
     5401 gaacgaacgg atctggtttg ccctcgctgc gtacaatatg ggctatgcgc atatgctgga
     5461 tgcccgcgcc ctgacggcaa aaaccaaagg gaatcctgac agttgggctg acgtaaaaca
     5521 gcgtctgcct ttacttagcc agaaacccta ttacagcaag ctgacttacg gctacgctcg
     5581 tgggcatgaa gcctacgctt atgtcgaaaa tattcgtaag tatcagatta gcctggtggg
     5641 ttatctgcaa gagaaagaga agcaggctac agaagcggcg atgcaactgg cgcaggatta
     5701 tccggcggta tcgcctacgg agttgggcaa agagaaattt ccttttctct cgtttctttc
     5761 ccagtcgtca tcaaactatt tgacccattc tccctctctg ctgttttcca ggaaagggag
     5821 tgaagagaaa caaaattaat ccgtcgaaga ttgcgctttt ttctgcgctt taatttcctg
     5881 gcggcgcatg cgaaagaagt cactgagcaa cgccgcgcac tcatccgcca gtattccttc
     5941 cgtaatttcc actcggtgat tcatacccgg atgatgcagc acatccatta aagatcccgc
     6001 agcgccagtt ttcgcgtcac gcgcaccaaa gaccacgcga ccaatgcgac tgtggatcat
     6061 cgctccggca cacattacac atggttcaag cgtgacatac aacgtggcgt cgatcagacg
     6121 ataattttgc atcaccagac caccctgccg cagggccatg atttctgcat gtgcggtggg
     6181 atcatggcga ccaatcgggc ggttccagcc ttcgccgatt acccgattgt tatgcactaa
     6241 taccgcgccg accggcactt cccgctcatc ccaggcacgt ttcgccagcg tcagcgcgtg
     6301 acgcatccag tattcgtggc taaattcgac ttcagacaaa aagaaaactc cggttataaa
     6361 agcgcggcgc attatacacg gacgctatgc tttactctat tccagttgct ggagttcacc
     6421 gcgcggggtt actcgccaac gatgctgaca gaaataaagc agcggattgt cctgattact
     6481 gtcgctatag ccactgtaca gccgcagcgg agtgccgatt ttgcgctcca gttgtgcgac
     6541 cttttcatgt cccagacaac gcatcgtcaa tacccaacca ccatagccac gctgaatttg
     6601 gctggcgata agattaaccc gcggcagcca gggcgtatcg aaataaaccg cttcaaccag
     6661 cggctgcgga gagccggtaa tcaaccagat atcagcatcg gaacttaaca ggtaggtggt
     6721 taatcgctcc tgaaccagcg gaaaggcggt aacattgtcg cgaaaccagc gcacgaaatc
     6781 ggcctgcaac gtctgtaaac gtgcttcgct gtgaccaaaa gtgcaccccc acagaagcag
     6841 actcatcggc cagcgtgccg cacgaccttt tatcaataac gcaatggcta taatcggtaa
     6901 caacggcagg acaagtaacg cattcagcgg ttggcgacgt agtaaatagc gcagaaaact
     6961 gccgaacata tcctgctgat gcaatgttcc atctaagtca aaaaacacca cacgacgctc
     7021 gtgagttgcc aaaccatact cctctggact gaaacatcct gattaatttt cgctactaca
     7081 tagcctaaca gatagatcat cacttttccg gcaacaatcg accgcatcat ggctaactgg
     7141 aatttttaat tcatggcaat tagcggcaat ggaatataaa attcactcgc gtgtgtctca
     7201 tattgcctgt tgtcgccact atttcatcgc gaggaaagat atgaacggct tacttcgtat
     7261 ccgtcagcgt taccaggggc ttgcccagag tgataaaaaa ctggcggatt atctgctgct
     7321 acaacctgat acggcgcgcc atttaagctc tcagcaactg gccaacgaag ccggagtcag
     7381 tcagtccagc gtcgtgaagt tcgcgcaaaa actcggctat aaaggttttc cggcgcttaa
     7441 gttggcgttg agtgaagcgc tggcaagcca gccggaatca ccctccgtgc ccattcataa
     7501 ccaaatccgc ggtgatgatc cgttacggct ggtcggcgaa aaactgatta aagaaaatac
     7561 tgccgctatg tacgcaacgc taaacgttaa tagtgaagag aaactgcatg aatgcgtaac
     7621 aatgttgcgc tctgcgcggc ggataattct gaccggtatt ggcgcttcgg gtctggtggc
     7681 gcaaaacttt gcctggaagc tgatgaagat tggcttcaat gctgccgcag tgcgcgatat
     7741 gcatgcgcta ctcgcaacag tacaggcgtc gtcccctgac gatctgttat tagccatttc
     7801 ctacaccggt gtacgacgcg agttaaacct ggcggcagat gagatgctgc gagtgggcgg
     7861 aaaagtgctg gcgattaccg gctttactcc gaatgccctg caacagcgtg cttctcattg
     7921 cctgtatacc attgccgaag aacaagcgac aaacagtgct tcaatctctg cttgtcacgc
     7981 tcagggaatg ttaacggatt tgctgttcat tgcgctgatt cagcaggatc tggaactggc
     8041 accagaacgt attcgtcata gtgaagcgct ggtgaaaaaa ctggtctgag taaagaatgc
     8101 gcgtataatg cccgcccggt ttgtgttgtt ttgagagttt ccttatggcg ttgttaatca
     8161 ctaaaaaatg catcaattgt gatatgtgtg aacccgaatg cccgaatgag gcgatttcaa
     8221 tgggagatca tatctacgag attaacagcg ataagtgtac cgaatgcgta gggcactacg
     8281 agacaccaac ctgccagaag gtgtgcccga tccccaatac tattgtgaaa gatccggcgc
     8341 atgtcgagac agaagaacag ttgtgggata aatttgtgct gatgcaccac gcgga
//
LOCUS       DAPHBJ010000187         8385 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.189, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000187 DAPHBJ010000000
VERSION     DAPHBJ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.189"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            301..1641
                     /gene="dcuB"
                     /locus_tag="RHZ76_003740"
     CDS             301..1641
                     /gene="dcuB"
                     /locus_tag="RHZ76_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HDV2814308.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYSIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            1719..3365
                     /gene="fumB"
                     /locus_tag="RHZ76_003741"
     CDS             1719..3365
                     /gene="fumB"
                     /locus_tag="RHZ76_003741"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDV2814309.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYRFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            3488..4117
                     /locus_tag="RHZ76_003742"
     CDS             3488..4117
                     /locus_tag="RHZ76_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HDV2814310.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(4256..5677)
                     /gene="melB"
                     /locus_tag="RHZ76_003743"
     CDS             complement(4256..5677)
                     /gene="melB"
                     /locus_tag="RHZ76_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HDV2814311.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(5780..7135)
                     /gene="melA"
                     /locus_tag="RHZ76_003744"
     CDS             complement(5780..7135)
                     /gene="melA"
                     /locus_tag="RHZ76_003744"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDV2814312.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHHEALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKKASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            7418..8326
                     /gene="melR"
                     /locus_tag="RHZ76_003745"
     CDS             7418..8326
                     /gene="melR"
                     /locus_tag="RHZ76_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HDV2814313.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
BASE COUNT         2152 a         2205 c         2056 g         1972 t
ORIGIN      
        1 tgagaacaaa aaatagacca ataaatatca ataagataga agcaaacaaa acattaacat
       61 ctgcgcagta caaactataa acccatcgcc agagagtctt tctctctgaa aaagccgctt
      121 atcacagtgc ataaatttgc cgctgcttta atcagccaat attcactgtg aggtatttgc
      181 taaagccggt aacgaccaaa cggatattta gtcaggctct gaaaacagtt catacaaaac
      241 agaacgtgac tgtgatctat tcagcaaaaa tttaaatagg attatcgcga gggttcacac
      301 atgttattta ctatccaact tatcataata ctgatatgtc tgttttatgg tgcccgaaag
      361 ggtggtatcg cgctgggttt attaggcggt atcggtctgg tcattctggt cttcgtcttc
      421 caccttcagc caggtaaacc accggttgat gtcatgctgg ttatcattgc ggtggttgcg
      481 gcatcggcga ccttgcaagc ttcgggcggt cttgatgtca tgctgcaaat tgccgagaag
      541 ctgctgcgcc gcaacccgaa atatgtctca attgtcgcgc cgtttgtgac ctgtacactg
      601 accattcttt gcggtacggg tcatgtggtt tacaccattc tgccgatcat ctacgacgtc
      661 gccattaaga acaacatccg tccggaacgt ccgatggcgg caagttctat cggtgcacag
      721 atggggatta tcgccagtcc ggtgtcggtt gcggtcgtgt ctctggttgc aatgctgggt
      781 aatgtcacct ttgatggtcg ccatcttgag ttcctcgacc tgctggcaat caccattcca
      841 tcgacgttaa tcggtatcct ggcgatcggt atcttcagct ggttccgcgg taaagatctg
      901 gataaagacg aagagttcca gaaattcatc tccgtaccgg aaaaccgtga gtatgtttac
      961 ggtgataccg cgacgctgct agataaaaaa ctgccgaaaa gcaactggct ggcaatgtgg
     1021 attttcctcg gggcaatcgc tgtagtcgca cttcttggtg ctgattcgga cctgcgtcca
     1081 tccttcggcg gcaaaccgct gtcgatggta ctggttattc agatgtttat gctgctgacc
     1141 ggggcgctga ttattatcct gaccaaaacc aatcccgcgt ctatctcaaa aaacgaagtc
     1201 ttccgttccg gtatgatcgc catcgtggcg gtgtacggta tcgcatggat ggcagaaacc
     1261 atgttcggtg cgcatatgtc tgaaattcag ggcgtactgg gtgaaatggt gaaagagtat
     1321 ccgtgggcct attccattgt tctgctgctg gtttccaagt ttgtaaactc tcaggctgcg
     1381 gcgctggcgg cgattgttcc ggtcgcgctg gcgatcggcg ttgatccggc atacatcgtg
     1441 gcttcagcac cggcttgcta cggttattac atcctgccga cttatccgag cgatctggca
     1501 gcgattcagt ttgaccgttc cggcaccacc cacatcggtc gcttcgtcat caaccacagc
     1561 tttattctgc cggggttgat tggtgtgagc gtatcgtgcg tcttcggctg gatcttcgcc
     1621 gcgatgtacg ggttcttata aatgcacttt gcgtgccgcc cgtgactacg cggcacgcca
     1681 ttttcgaata acaaatacag agttacaggc tggaagctat gtcaaacaaa ccctttatct
     1741 accaggcacc tttcccgatg gggaaagaca ataccgaata ctatctactc acttccgatt
     1801 acgttagcgt tgccgacttc gacggcgaaa ccatcctgaa agtggaacca gaagccctga
     1861 ccctgctggc gcagcaagcc tttcacgacg cttctttcat gctccgcccg gcacaccaga
     1921 aacaggtcgc ggctattctt cacgatccag aagccagcga aaacgacaag tacgtggcgc
     1981 tgcaattctt aagaaactcc gaaatcgctg ccaaaggcgt gctgccgacc tgccaggata
     2041 ccggcaccgc gatcatcgtc ggtaaaaaag gtcagcgcgt gtggaccggt ggcggtgatg
     2101 aagaagcgct gtcgaaaggc gtctataaca cttatatcga agataacctg cgctattcac
     2161 agaatgcggc gctggacatg tacaaagagg tcaacaccgg cactaacctg cctgcacaaa
     2221 tcgacctgta cgcggtagat ggcgatgagt acagattcct ttgcgttgcg aaaggcggcg
     2281 gctctgccaa caaaacctat ctctaccagg aaaccaaagc tctgctgacg ccgggtaaac
     2341 tgaaaaactt cctcgtcgag aaaatgcgca ccctcggtac cgcagcctgc ccgccgtacc
     2401 atatcgcgtt tgtgattggc ggcacttctg cagaaaccaa cctgaaaacc gtcaagttag
     2461 caagcgctca ctattacgat gaactgccga cggaagggaa cgaacatggt caggcgttcc
     2521 gcgatgtcca gctggaacag gaactgctgg aagaggccca gaaactcggt cttggcgcgc
     2581 agtttggcgg taaatacttc gcgcacgaca ttcgcgttat ccgtctgcca cgtcacggcg
     2641 catcctgccc ggtaggcatg ggcgtctcct gctccgctga ccgtaacatt aaagcgaaaa
     2701 tcaaccgcga aggtatctgg atcgaaaaac tggaacacaa tccaggccag tacattccac
     2761 aagaactgcg ccaggccggt gaaggcgaag cggtgaaagt tgaccttaac cgcccgatga
     2821 aagagatcct cgcccagctt tctcaatatc cggtttccac tcgtttgtcg ctcaccggca
     2881 ccattatcgt gggccgagat attgcacacg ccaagctgaa agagctgatt gacgccggta
     2941 aagaacttcc gcagtacatc aaagatcacc cgatctacta cgcgggtccg gcgaaaaccc
     3001 ctgccggtta tccatcaggt tcacttggcc caactaccgc aggtcgtatg gactcctacg
     3061 tggatctgct gcaatcccac ggcggcagca tgatcatgct ggcgaaaggt aaccgcagtc
     3121 agcaggttac cgacgcgtgc cataaacacg gcggcttcta cctcggtagt atcggcggtc
     3181 cggcggcggt actggcgcag cagagcatca agcatctgga atgcgtcgct tatccggagc
     3241 tgggtatgga agctatctgg aaaatcgaag tagaagattt cccggcgttt atcctggtcg
     3301 atgacaaagg taacgacttc ttccagcaga tcgtcaacaa acagtgcgcg aactgcacta
     3361 agtaacctct tcggcccagc gcctggcagc atgctgccag gtgatccccc tgggccacct
     3421 cttttgcgat tgtaatttca cgcttgctgg tgaatagtca gtattttccc tgacttgcga
     3481 actcaccatg acccgcacac tcaagccgtt aattcttaac accggcgcac tggcgctaac
     3541 gttaatcctg atttataccg gcatttcggc ccatgacaaa ctcacctggc tgatggaagt
     3601 gacaccggtg attattgtcg tgccgctact gcttgccacc gctaaacgtt atccgttaac
     3661 gccgctgctc tatacgctca ttttctttca cgccatcatc ctgatggtcg gcgggcaata
     3721 cacctacgcg aaagtccccg ttggttttga ggtgcaggaa tggctggggt tgagccgtaa
     3781 tccgtatgac aagctggggc attttttcca ggggctggtg cctgcactgg tggcacgtga
     3841 gatccttgtg cgcgggatgt acgttcgcgg acgtaaaatg gtggcgtttc tggtctgttg
     3901 cgtggcgctg gcgataagcg cgatgtatga attaatcgag tggtgggcgg cgctggcaat
     3961 gggccaggga gcggatgatt ttctcggcac ccagggcgac cagtgggaca cgcaatccga
     4021 tatgttctgc gcgctgcttg gcgcattaac cacggtgata ttcctcgctc gctttcattg
     4081 ccgtcagcta cggcgctttg gcttgataac cggataacaa cgtgcacttt gttgatgccg
     4141 gatgcggcgt gaattcctta tccggtctac aaaatttgca aatccaaagc attacattaa
     4201 gacgtggcct gataaacata tcgcatcagg ccattttcgt tagctattgt ccacgtcagg
     4261 ctttcacatc actcactgca ccgaccggaa tatcagcatg aacaggctcg ggcggtactt
     4321 tgcgatattt atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca ttgaggcgat
     4381 agaaacggaa gtagagaatc agcgttacca tgaaaaacag agtcggtaga gcaatcataa
     4441 taaactgcat acctaatagg gcttgcgtag actgttcaac attcggtaca tagccaatca
     4501 tccctaacac aaccgcaatg aaaaaagccg caaaggctga accgcccttc accaccatag
     4561 tctgcacgga gtaagcgatg ctttcacagc gtacgtgcag tttatattca ccgtaatcaa
     4621 cggtatctgc caccatgatg acctgtaata cccagaaaag cgccgttccc acattcagca
     4681 aaatacccgc aatcacaatg aggacgacgt tgtgatagct cattaatgcc attaacagga
     4741 gaacaccaca gcttaacacc ggaagaatag atgctccggc ccataaaatg cgtcgggata
     4801 atgatttaac taagcgtggg aagaatacta acgtcaccag gttagcagct cccgcatacg
     4861 acagataata ggggaacaaa tccgcatcac cgataacata tgagaaataa tagatagcaa
     4921 agccggtaat aatgttgctg gctacattat aagcaagagc catacccaag aggcatgaaa
     4981 gctgatcgtt tttataaata agcgcaacga tggctttaag tgtcagatgg cttccttcag
     5041 cagacggttg attgtctgac gaaaagactt catgcacatt gcgcagagtg atgatggttg
     5101 aaacaataaa aaaggcgatc agtaccagtg tgaacatctg aaagccaaat ccccgatcgc
     5161 caccgccgac ataattaaca aatggtagcg tcacacctgc cgtaacaaag cctgccagac
     5221 tggcaaaaaa acgcggataa ggaaccagtt gttcgcgctc acgtttatcg agcgttatgg
     5281 ttggaaccag cgaccagaag ggaatatcca taatggtgta agtcatgccc cagaggatgt
     5341 aggtcacgca aacaaagaca atctgagtag taccttcaaa cagatgcgca ctaaagagga
     5401 gaaataagat tacagagttt gccaacgtac cgatcaggat ccagggtttg aacttacccc
     5461 atcgcgatcg cgtagcattt acaatccatc ccataatcgg atcgttaata gcatcccaga
     5521 tcctcgccac cagaaacaaa gtaccgacca aacccacaga cagcccgacg acatcggtgt
     5581 agtaatacat gaggtacata tacacaatgc cgatcgcaaa atccttcccg aacgctccaa
     5641 atccataact gagttttgta gtcattgaaa tgctcatagg gtatcgggtc gctgttacca
     5701 acgcccgcct tctgttattg gtatcgtcag aggtgtgcgc cggaataaat cccccggcgc
     5761 agtagcgttt agtcgcgttt taacggtgca accagcctgg cagccagtcg ccgtgggcgg
     5821 caatcaggtc gtcaacaaga gcatatattt cgtcaatgcc cagcacggcg gcagtatgcg
     5881 ggtccatcat cgcggcgtgg taaacacggt cgcgattttc cgtaagaata gcttcggtca
     5941 gcagcgtctg tacgttgatg ttggtttgca tcagggcggc cagatgcgaa ggtagcgtac
     6001 cgactttggt cggctgaatg ccattagcat caaccagaca ggctacttcc acgcaacatc
     6061 cttgtggcag gttatcaatc aaaccatcgt tacggacgtt gccgtaaatc acactcggct
     6121 cgccagtcca gatagcgttc atgattgtgc tggcatattc ccgtgacggt ttaatatcaa
     6181 tccgggaggc ttttttatac tcctccagct ctttatgcca gttcgccagc tgctcgacgc
     6241 agcgtttcgg gtactcatcc agcggtactt tataacgctc aatcaaatcc tcacgacctg
     6301 gcttaataaa ccacggtgtg tactcagcaa aatgttctga cgattccgtg acgaaatagc
     6361 ccagcttttt gaacatttcg tagcgcacaa tattctggca gcgagtattg ccatgaatat
     6421 tcggcttcgg tgcctgccct gcttcataag ccgccagcag ttccgggtag agattcacat
     6481 aactgccgtc ggcggttttg cgctccagct ccaggtaaaa cgccatatgg ttgatacctg
     6541 cgcaacggta acgcagcgta gctgggtcga tattgaggtc acgcgccaac tcttccgccg
     6601 ttccctgcac cgaatggcac agcccgacct gtttgatatg cggatagcgg gcatacatcg
     6661 cccaggtatt catcgccatt gggttaacat agttgagcat ggtggcatcg gggcagactt
     6721 ccgtcatgtc ctcgcaaatt tgccacagat gcggaatggt acgtagcgcg cgcataatac
     6781 cgcccggccc caacgtatcg gcaatggttt gttccagacc atgccgctta cagacctcga
     6841 aatcagtcac cgtgcaaggt tcataaccgc caatctgaaa tgccaccacg acaaaatcgg
     6901 catcctctaa ggcttctttc tgttgggtgt ggcaggtgat tttgccgctg gcccctgctg
     6961 aatccatcag cttacgcacc acaatatgcg actcttccag gcgggtgggg tcaatgtcca
     7021 tcagggcaat atgcgccgtt ttcagcgcct cgtgatggaa cacatcacca agaatatttt
     7081 taacgaaaat cgtcgaacca gcgccgataa atgtaatttt gggtgcagac atcatgcaga
     7141 tctcctggct tgcttgaata acttcatcat ggcaggcgaa tctgcagaaa acctccggct
     7201 tccaggttga gcattcccat aaactcagat cctgcgtgaa gcagcagtaa atctgagttt
     7261 atgggaataa attacctgaa aagcagagtt ttcggcctca tgatactcgg agagtcccct
     7321 cgcccctctg ggaagagggt gaaaaccgtg ctcccactcg cagtcatcct ccctcactcc
     7381 tgccataatt ctgatattcc aggaaagaga gccatccatg aatacagata cgtttatgtg
     7441 cagcagcgac gaaaaacaga cccgcagccc gctgtcgctg tattcggaat accagcggat
     7501 ggagattgag tttcgcgcac cgcatatcat gcccaccagc cactggcatg gtcaggtcga
     7561 agtgaatgtg cctttcgatg gcgatgtgga atacctgatc aacaatgaaa aagtgaatat
     7621 caatcagggt catatcacgc tgttctgggc ctgtacaccg caccaactaa cagataccgg
     7681 aacctgtcag agcatggcga tttttaatct gccgatgcat ctgtttctct cctggccgct
     7741 ggataaagac ctgattaacc acgtcactca cggcatggtg atcaaatcac tggccacaca
     7801 gcaacttagc ccgtttgaag tgcgccgctg gcagcaggaa ttaaacagtc cgaacgagca
     7861 aattcgccag ctcgccattg atgaaattgg cctgatgctc aagcgattta gcctctctgg
     7921 ctgggaaccg attctggtca ataaaacctc gcgcacacac aaaaacagcg tctcgcgcca
     7981 tgcgcaattt tatgttagcc agatgctggg ctttattgcc gaaaactatg atcaggcgct
     8041 gaccatcaac gatgtggctg agcacgtcaa acttaacgcc aactatgcaa tggggatatt
     8101 ccagcgggtc atgcaattga cgatgaaaca gtacattacc gcgatgcgca tcaaccacgt
     8161 tcgcgcgtta ctgagcgata ccgataaaag tattctcgat attgccctga cggcaggctt
     8221 tcgttcaagt agtcgttttt acagcacgtt cggcaaatat gtcggcatgt cgccgcaaca
     8281 ataccgcaaa cttagccaac agcgccgcca gacgtttccc ggctaaagac gatatcagta
     8341 tcagccaaaa aaatagttta gccgtcgata ataagcagtg aaaat
//
LOCUS       DAPHBJ010000188         8304 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.190, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000188 DAPHBJ010000000
VERSION     DAPHBJ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.190"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            82..1860
                     /locus_tag="RHZ76_003746"
     CDS             82..1860
                     /locus_tag="RHZ76_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HDV2814314.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTFGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISHIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVKILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1900..2379)
                     /gene="xdhC"
                     /locus_tag="RHZ76_003747"
     CDS             complement(1900..2379)
                     /gene="xdhC"
                     /locus_tag="RHZ76_003747"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HDV2814315.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2376..3254)
                     /gene="xdhB"
                     /locus_tag="RHZ76_003748"
     CDS             complement(2376..3254)
                     /gene="xdhB"
                     /locus_tag="RHZ76_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HDV2814316.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3265..5562)
                     /gene="xdhA"
                     /locus_tag="RHZ76_003749"
     CDS             complement(3265..5562)
                     /gene="xdhA"
                     /locus_tag="RHZ76_003749"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HDV2814317.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            5977..6732
                     /locus_tag="RHZ76_003750"
     CDS             5977..6732
                     /locus_tag="RHZ76_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDV2814318.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSITRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6811..6884
                     /locus_tag="RHZ76_003751"
     tRNA            6811..6884
                     /locus_tag="RHZ76_003751"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6843..6845,aa:Gly,seq:ccc)
     gene            7049..8233
                     /locus_tag="RHZ76_003752"
     CDS             7049..8233
                     /locus_tag="RHZ76_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV2814319.1"
                     /translation="MKLTARQVETAKPKEKPYKLADGGGLYLLVNPNGKRYWRLKYRS
                     LGKEKLLAIGVYPDVSLAEARSKREDAKRTLAAGNDPSLERKIEKLSRQQDAENSFEA
                     ITREWYQRRYDRWSVSYREEMMRTFEKDVFPYIGHRPIKDIKPMELLAVLSKIEARGA
                     TEKVRKVRQRCGEVWKYAVVTGRAEYNPAPDLASAVTPHEKEHYAFLIQDELPEFLRT
                     LNSYAGSVIVKIAMQLLILTGVRPGELRQAEWQEFDFERKVWNVPAERMKMRRPHLVP
                     LSSQAIDLFNQLKPMTGAGALLFPGRNNPKKPMSDMALTVLVRRIGYAGRVTGHGFRH
                     TMSTILHEQGFNSAWIELQLAHVDKNSIRGTYNHAQYLEGRREMMQWYGDYVEACLTK
                     FV"
BASE COUNT         2098 a         1943 c         2154 g         2109 t
ORIGIN      
        1 gcctgagtta atatcatcat gatctgtgaa ccatcaacgt cttcaatgat cgacttttaa
       61 gtaagtctgg aaggtaaagc tatggagctt gctactacgc agtcagtatt gatgcaaatt
      121 caaccgacaa ttcagcgttt tgccagaatg cttgccagcg ttttgcagct tgaggttgag
      181 atcgttgatg aaaacttgtg tcgcgtcgcc ggaacgggcg cgtatgggaa gtttcttggt
      241 cgccagttga gcggcaactc acgcctgctc cgccacgtcc tggaaacgaa aactgaaaaa
      301 gttgtgacac agtctcgctt cgatcccctt tgcgaaggtt gcgatagtaa agaaaattgc
      361 cgcgaaaaag catttctggg tacgcctgtc attttacagg atcgttgtgt tggggtgata
      421 agtttgattg ccgttaccca cgagcaacaa gagcatatca gtgataattt acgcgaattt
      481 tctgattatg ttcgccatat atccaccatt tttgtttcga aacttctgga ggatcagggg
      541 ccaggagata acatcagtaa aatattcgcg accatgatcg ataatatgga tcagggcgta
      601 ttagttgttg atgatgaaag tcgggttcag tttgttaatc agactgcctt aaaaacattt
      661 ggtgttgtac aaaataatat tattgggaaa cctatccgtt tcagaccatt aacatttgag
      721 agtaatttta ctcatggaca tatgcagcat attgtttcgt gggacgataa aagtgaatta
      781 atcattggtc aattgcataa cattcagggc cgacaattat ttttaatggc atttcaccaa
      841 tcgcatacca gtttttctgt agcaaatgca cctgatgaac cacatattga acaattggtt
      901 ggcgagtgcc gtgttatgcg gcaattaaaa cgactcatta gccatattgc acccagccca
      961 tccagcgtta tggtggttgg tgaaagcggc acgggtaaag aagtcgtcgc ccgagcaatc
     1021 cataagttga gcggaagacg gaataaaccc tttattgcta tcaactgtgc cgcgattccg
     1081 gagcagcttc tggaaagcga actgttcggt tatgttaaag gcgcatttac tggcgcttct
     1141 gccaacggta aaacagggtt gattcaggcg gcgaatacgg gcacgctgtt tctcgatgaa
     1201 ataggtgata tgccattaat gttgcaggct aaattactgc gcgctattga ggcgcgtgaa
     1261 attctgccga ttggtgccag tagcccaata caagtcgaca ttcgcatcat ttctgcaact
     1321 aatcagaatt tggcccagtt cattgccgaa ggtaaattcc gcgaagatct cttctaccga
     1381 cttaatgtta tcccgataac tctgccaccg ctgcgtgaac gtcaggaaga tattgaacta
     1441 ttggtgcatt actttttaca tctgcatacc cgtcgtctgg gatcggttta tcctggcatt
     1501 gctcccgatg tcgtcaaaat attgcgtaag catcgttggc ccggaaacct gcgcgagtta
     1561 agcaatttga tggaatatct ggttaacgtg gttccttcag gtgaagttat cgacagcacg
     1621 ctattgccgc caaatctgct gaataatggc acaacggagc aaagtgatgt aacagaggtc
     1681 agtgaggcgc acctgtcact cgatgatgcg ggcggcacgg cgctggagga gatggaaaag
     1741 caaatgatcc gcgaggcgct ttcacgtcat aacagcaaga agcaagttgc tgatgaactg
     1801 ggcatcggca ttgctacgct ctatcgcaag attaagaaat atgagttgtt aaacacataa
     1861 cctcagtgaa tccggcctga attcaggccg gatatccttt tacttcgttt tctcgcaatc
     1921 cagaactgta tttacaatca tctgataccc cgtgcagcga caaagatttc ccgccagtcc
     1981 gcgacgaatt tccgtaatgg ttaatggctt ctcgcgtggt ttcgccagca ttgccgtggt
     2041 agccataatc aggccaggcg tacaaaaccc gcactgcact gcgccggatt tcgcataagc
     2101 ctgctgaaca tgagaaagtt ttccgccttt cgcttcacct tccagcgtgc ggatctcttt
     2161 tccttcagcc caggcggcaa ggtataagca actgtctatt gctgtgccgt cgaccaacac
     2221 cgtacaggca ccacattcac ccacgcagca cccttgtttg acacttagca gtccttgttc
     2281 gcggagtaat tccgagagcg gcgtgcctgg tgcggcgtga agctgaaaag gcatcccgtt
     2341 aatggtgcat tcgatggtaa ttgtttcgct gtgattcatt gcaattttcc ccccgccgcg
     2401 gcgacggctt cgctaatcac ttttttggtc atcgtctgga tgagatgcag acgaaactct
     2461 ttactggccc gccatgaaga acgcggggcg acatcttgca ggacagattc gctgatagct
     2521 tccagcgttt gcaggtttaa tggcgcattt tgtgcagtct gttcggcatg ttggcagcga
     2581 atcggcgttg gcgcggcaac accaaatgcc aggcgtaatt cgctgaaatt gccgttatcc
     2641 agtcggcaat gtgcggcgca gccaatcgtt gaaatatcca ttgcgtcgcg catggcatat
     2701 ttaaaatgcg cgctgcccgc gtgttctttc ggctgtggcg gaaaatgaaa ggcgacgagg
     2761 atttcgtcat gctcaagaga cactttgccc ggcccggtgt gaaagccatt aatcgggacg
     2821 aaacgaacac cgcgcgggga gtggatctcc agtttcgcgt cataaattag cgttggcgtg
     2881 gcagaatctg cgctggtggc accgttgcaa atatttccac cgtaggtagc gacgttacgg
     2941 atctgcggtc cagcaatgga cgtggccgca gcacataacg ccgggagatg acgttgagtt
     3001 ataggatctt ctattagctg ggtaaatgtc gttgcagagc cgatacgtag cgagccatct
     3061 tccgccagcg taattccccg cagctccgcc agattatgaa tatcaacaat atggcgataa
     3121 cggtcattgt gatggtggag ctgaatcagt acgtcagtgc caccggcgag cagtttggcc
     3181 tgcgggttgt cagccagcag gttgatggca tcggcaaggg ttgctgcgcg atggtaagaa
     3241 gcaaaatcaa acatgatgtt atcctcaaat caatcctgcc agatggaact cttcatataa
     3301 ccgttttggc gtcagcggca gtgtattgat tgcaacaccg gtagccatct tcaccgcgtt
     3361 acgaatagca gcggcaacag gaattattgg tggctcaccc agtgacttat gtccgtatgc
     3421 ggattgcggc tcattgattt cgacgaacgc gctttccagt tgtggcagat ccggcatggt
     3481 cggcattttg taatccagca gattggggtt acggaccacg ccgcttttag catcgatgat
     3541 catctcttca aatagcgccc agccaatgcc cattcccatt ccgccgtgta cctgaccttc
     3601 tgccagcagt ggattaagaa tatgccctga atcatgaacg ttgaggatgc ggttgatggt
     3661 gactttgcac agcgcaatat cgaccgtcag atcaacaaag gtacagccaa acgccggtgg
     3721 gttagtggtg gttttgatgg agctttcagc agagagctgc ccgccgcgtt cagggtggta
     3781 gaaagcgtcc atcgccaaat cttttaacga cattaacggc tcttccggtc gttcaaccag
     3841 cacgatatgg ccttttatca gggtcagatt catcgctgac tgatgtagca tgactgcggc
     3901 gtgagcgatg attttctctt ttaataatag tgccgcactg cgcagcgcag gcgcggcaac
     3961 atagctctgg cgtgaggcaa atgcgccggg atcgaacggc gtaacgtcgg tatcttgagt
     4021 tgaaataacg cgaacgtcgc tgaccggaac ccccacggtt tctgccacca tttgcgagaa
     4081 gacggtgtcg gcaccctgac cgatttccgt cgcgccgctt tgcacgttga tggttccatc
     4141 ctgattcatc agaaggcgcg cgcctgctat ttctacgccg acaggccagg tgttagaggt
     4201 gtagctaaaa caggcgacgc caacgccgcg gcgcaaattg ccttgctggt tctggcattc
     4261 tgcacggcgt ttttcccatt caaagatttt ccggcctttt tcaagacact ccggcaaccc
     4321 tgcgctgtaa atacgtttgc ccgtgagcgg attagcatct ccttcgcggg cggcgttgcg
     4381 taaacgaatt tcaacaggat caatacctaa cgctgtcgcg gcgtcatcaa gcatagactc
     4441 aacggcaaat acgacttgtg gcgcgccata accacgcatc gcaccagccg aggggaggtt
     4501 ggtatagcag gtctttgaac tgtaagcgta ggcacaacga ggataaaggt aagcgacttt
     4561 attccccccc gcagaagcga tggagtgccc gtgagatgca taagcgccgg tgttagacag
     4621 aacatccaga ctataacctt tcaatgttcc gtcgcggttc acgcccattt gcccgtcaat
     4681 ggtaaaagcg tggcgggtac gggttgcgag gaaacactct tcacggctaa gggaaacttt
     4741 caccggaatg ccgccaagct tgctggtcag gaatgccgcc attggctctt ccagtacatc
     4801 ctgtttatta ccaaaaccgc caccgacaaa tggtttgatg actcgtacgc atgaccaggg
     4861 aatatccagc gcctgaccaa ccacgcggcg aacaatgtgc gggatctggg tgctggaaac
     4921 gatggtaatt cgcgagtcat cctccatcca cgccagcgat gttacgcttt ccatgtgaca
     4981 atgttgaata acgggggtct gatagtgccc ctgtacctgg tagtcggcgg catcgattgt
     5041 ttgttggaca ttacccgtcg acatcgtgct ttgtttcagt aaattgccac cgttatggat
     5101 tggtgcagcg tcttctgcca gcgccgcttc tggcgtggtg ataacgggta attcttgcca
     5161 ctcaatgctg accaattgcg ccgctttttc tgccgtgagt tcatcgcggg ccacgacgat
     5221 ggcaacggcg tcaccatgat gacgaacatg gcgagttagc agtgcgcgat cggcggtatc
     5281 gcgcttgttt tcgtcaagtg tccaggcatg cccagctgta gcgaatggaa tatcaggcac
     5341 atcttcccag gtaaaaatcg ccagtacgcc cggcaaactt ctggcttgtt catcattaat
     5401 acttacggca taaccatgtg cgatagggct acgtacatat ttcgcataac acatgcccgc
     5461 cataacataa tcgtcagtat atcgtgcccg cccggtgacc ttagcaatgg catcgacgcg
     5521 catgcatgat tcacccgtag cggttgcttc ccgcgcttcc atagaaatcc cctcgatttg
     5581 aacatttggt tcttatggca aagatttagt ctaagcaggc aagatttacg ccagaaacga
     5641 cgataagcgt taggggaggt aggaagtgtg atgacgttaa aaaaacacag atatgatacg
     5701 aaacaggact ctgaataggg gcagtgatac gtatcaatat gagtggcgat aaaatcaatg
     5761 tgataaattg ggattattac gcatcaggat gggaaatagg gtaaaaagtc aggaagttcg
     5821 atacctctac agcgaatatt ttgctgatga tacttccctt tgccaaatga aattaacctc
     5881 tttctctatt accacgtttt tccagaagca agagattggg tatcaagagg ctggctgcta
     5941 tgataaggcg tcttgttttt taagcgagga aagattttga gtgcgggacg cctgaataaa
     6001 aaatctctgg gtatcgtgat gttgttatcg gttggactgc ttttggcggg ctgttcgggt
     6061 agcaaatcat ccgatacagg aacgtattcc ggctccgttt acaccgtgaa acggggggat
     6121 acgctatatc gtatttcgcg caccacggga accagcgtaa aagaactggc gcgactgaac
     6181 ggcatttccc ccccttacac cattgaagtt ggtcagaaac taaaactggg tggggcgaaa
     6241 agtagcagta ttacacgtaa atcaaccgcc aaatcaacga ccaaaaccgc atcggttaca
     6301 ccgtcatcag cggtaccgaa atcatcctgg ccgccagtag ggcaacgttg ttggttatgg
     6361 ccaacgacag ggaaagttat catgccgtat tcgacagcag atggcggcaa taaagggatt
     6421 gatatctcag ctccacgggg tacacctatt tacgccgcgg gtgcaggaaa ggtggtgtat
     6481 gtgggcaacc agctgcgtgg ctacggtaat ctcatcatga ttaaacacag tgaagattac
     6541 attacggctt acgcccataa tgacacgatg ctggtaaata atgggcaaag cgtgaaggct
     6601 gggcaaaaaa tcgccactat ggggagcacg gatgcggcat ctgttcgcct gcatttccag
     6661 attcgttacc gtgcaacggc aattgatccg ctacgttact tgccgcctca gggcagcaag
     6721 ccaaaatgct gatggcgaat taatcagcag tcagcagcgt ggcacttgct aaggagagcg
     6781 taaggtttat aatgccttac gcatctcgaa gcgggcgtag ttcaatggta gaacgagagc
     6841 ttcccaagct ctatacgagg gttcgattcc cttcgcccgc tccaaactca actcccctga
     6901 agtctaccaa actcaacttt tccttgataa atcagcagtt atagcattct attagtctat
     6961 tgaactcaat tgtactcccc cagggtcaag ctcaatgtgg gggtatggat gggggtataa
     7021 ttgtaccccc tgctgagagg tacccccaat gaagctgaca gctcgtcagg ttgaaactgc
     7081 aaaacccaaa gagaagccct acaaactggc tgatggtggt ggtctttacc tgctggttaa
     7141 tcccaatggt aaaagatact ggcgtctcaa ataccgttcg ttaggtaaag agaagctgct
     7201 cgctattggc gtttaccccg atgtttcttt agctgaggcc agaagtaagc gtgaggatgc
     7261 aaaaaggaca ttagcggcag gtaacgatcc ttctcttgaa cgaaaaattg agaaacttag
     7321 tcgtcaacaa gacgctgaaa attcgtttga ggctatcact cgagagtggt atcagcgtcg
     7381 ctatgatcgc tggtctgtct cttatcgtga agagatgatg agaacgtttg agaaggatgt
     7441 atttccttat atcggccacc gaccaatcaa agatatcaaa ccgatggagt tgcttgctgt
     7501 tctttcaaag atagaagctc gaggagcaac tgagaaggtc cgtaaggttc gccagcgttg
     7561 tggagaggtc tggaagtatg ccgttgtgac tggcagggcc gagtacaatc cagcacctga
     7621 tctagccagc gccgtaactc ctcatgagaa agagcactac gcttttttaa tccaggatga
     7681 attgccagag tttcttcgga ccctgaactc ctatgccggt agtgtgattg ttaagattgc
     7741 gatgcaatta cttattttga ctggagtaag gcctggggaa ttgaggcaag cggagtggca
     7801 ggagtttgat tttgagcgga aggtttggaa cgtgccagca gagcgcatga aaatgcgccg
     7861 ccctcatctg gttccattat caagccaggc aattgaccta tttaatcagc ttaaacctat
     7921 gacgggagca ggagcgttgc tgttcccagg acgtaataat cctaagaaac cgatgagtga
     7981 tatggcattg actgtgttgg taaggcgcat cggctatgca ggacgcgtta cagggcatgg
     8041 attccgccac acgatgagta ctattttgca tgagcaggga tttaactcgg cctggattga
     8101 gttacagctg gctcacgtcg ataagaactc aattcgtgga acgtataacc atgctcaata
     8161 tctggagggg aggagggaaa tgatgcagtg gtatggggat tatgtcgaag cgtgtttaac
     8221 taagttcgta taagtttgag aatagggaaa acgagttttt gatgtttttc ccttctctaa
     8281 ggtcattatt gttgttggct gtat
//
LOCUS       DAPHBJ010000189         8180 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.191, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000189 DAPHBJ010000000
VERSION     DAPHBJ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.191"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..329
                     /locus_tag="RHZ76_003753"
     CDS             <1..329
                     /locus_tag="RHZ76_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814320.1"
                     /translation="SPIFIETEDNLRTFNTNELTRNDNIFILFSSVDDGPVMVVSSQR
                     LHDMLNPTKDTNWNSTYIYKSRHEMLPVNLTQETLFSSKSHGKYALFPIFTASWRAHR
                     IMNKGV"
     gene            552..1049
                     /gene="ubiC"
                     /locus_tag="RHZ76_003754"
     CDS             552..1049
                     /gene="ubiC"
                     /locus_tag="RHZ76_003754"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HDV2814321.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1062..1934
                     /gene="ubiA"
                     /locus_tag="RHZ76_003755"
     CDS             1062..1934
                     /gene="ubiA"
                     /locus_tag="RHZ76_003755"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HDV2814322.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2089..4512)
                     /gene="plsB"
                     /locus_tag="RHZ76_003756"
     CDS             complement(2089..4512)
                     /gene="plsB"
                     /locus_tag="RHZ76_003756"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HDV2814323.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4683..5051
                     /gene="dgkA"
                     /locus_tag="RHZ76_003757"
     CDS             4683..5051
                     /gene="dgkA"
                     /locus_tag="RHZ76_003757"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDV2814324.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5161..5769
                     /gene="lexA"
                     /locus_tag="RHZ76_003758"
     CDS             5161..5769
                     /gene="lexA"
                     /locus_tag="RHZ76_003758"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HDV2814325.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5842..7167
                     /gene="dinF"
                     /locus_tag="RHZ76_003759"
     CDS             5842..7167
                     /gene="dinF"
                     /locus_tag="RHZ76_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HDV2814326.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7283..7492
                     /locus_tag="RHZ76_003760"
     CDS             7283..7492
                     /locus_tag="RHZ76_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDV2814327.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7534..8049)
                     /gene="zur"
                     /locus_tag="RHZ76_003761"
     CDS             complement(7534..8049)
                     /gene="zur"
                     /locus_tag="RHZ76_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HDV2814328.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1840 a         2006 c         2241 g         2093 t
ORIGIN      
        1 tttcacctat atttatagaa actgaagata atttaagaac ctttaacacg aatgaattaa
       61 cacgaaacga taatatcttt atcctgttct cctcagtcga tgatgggcca gttatggtgg
      121 taagctccca gcgcttacat gatatgttga atcctacaaa agataccaat tggaattcca
      181 cgtatatcta caaatccaga catgagatgt tgcctgttaa tcttactcag gaaacacttt
      241 ttagctccaa atctcatggt aaatatgcgc ttttcccaat ttttactgcg agttggcgag
      301 ctcatcgtat aatgaataag ggtgtttaag taaaggaaaa catcaccgtt cctggcatcc
      361 tggacggtga tgcccctacg gttgccctcg ccagcacggg catcggtaaa gcgtaaggtt
      421 caacatcgtt ttaccacttc atgcgattgt tgcgtttttg ttgcgtatta gatcacttaa
      481 tttgctttac atctcccgta aacacttttc tgcgatacaa tgcctttacg ttatgtaacg
      541 gagagttcgg catgtcacac cccgcgttaa cgcaactgcg tgcgctgcgc tattttaaag
      601 agatccctgc cctggatccg caactgctcg actggctgtt gctggaggat tccatgacaa
      661 aacgttttga acagcaggga aaaacggtaa gcgtgacgat gatccgcgaa gggtttgtcg
      721 agcagaatga aatccccgaa gaactgccgc tgctgccgaa agagtctcgt tactggttac
      781 gtgaaatttt gttatgtgcc gatggtgaac cgtggcttgc cggtcgtacc gtcgttcctg
      841 tgtcaacgtt aagcgggccg gagctggcgt tacaaaaatt gggtaaaacg ccgttaggac
      901 gctatctgtt cacatcatcg acattaaccc gggactttat tgagataggc cgtgatgccg
      961 ggctgtgggg gcgacgttcc cgcctgcgat taagcggtaa accgctgttg ctaacagaac
     1021 tgtttttacc ggcgtcaccg ttgtactaag aggaaaaaaa tatggagtgg agtctgacgc
     1081 agaataagct gctggcgttt catcgcttaa tgcgtacgga taagccaatt ggcgcgttac
     1141 tgctgctctg gccaacatta tgggcgttgt gggtggcgac accgggcgtt ccccagctct
     1201 ggatcctggc ggtgtttgtc gcgggtgtct ggctgatgcg cgctgccgga tgtgtggtga
     1261 atgattatgc tgaccgcaag tttgatggtc atgttaagcg cacggcgaac cgaccacttc
     1321 ccagcggcgc ggtaacagag aaagaggcgc gcgcgctgtt tgtcgtgctg gtactgattt
     1381 cgtttttact ggtgctgacg ctgaatacga tgaccattct gttgtcgatt gccgcgctag
     1441 cgctggcgtg ggtgtacccg tttatgaagc ggtataccca tctaccgcaa gtggtgctgg
     1501 gcgcggcgtt tggctggtcg attccaatgg cttttgccgc tgtgagtgag tcggtgccat
     1561 tgagttgctg gttaatgttc ctcgccaata ttctctgggc ggtggcttac gacacgcagt
     1621 atgcgatggt tgaccgcgat gatgatgtga agattggcat taaatccacg gcaatcctgt
     1681 tcggccaata cgataaattg attattggta ttttgcagat tggcgtactg gcactgatgg
     1741 cgatcatcgg tgagttaaat ggcttaggct ggggatatta ctggtcaatt ctggtggctg
     1801 gcgcgctgtt tgtttatcaa caaaaactga ttgccaaccg cgagcgtgaa gcctgcttta
     1861 aagcatttat gaataataac tatgttggtc tggtactatt tttagggctg gcaatgagtt
     1921 actggcattt ctgatgatgt aaaaaagccg gatgatcatc cggctttctt ctgggttgcc
     1981 tgatgcgcgg cgcttctcag gcctacacaa cacatcgcaa tttattgaat ttgcagatta
     2041 tggaaggccg gataaggcgt tttcgccgca tccggcaatt ctctctgatt acccttcgcc
     2101 ctgcgtcgca ctctcaatcg tcaaacgcac gtctgatgta atcaactccg ccagcaactg
     2161 ataaaccttc atcgtttctg ccggttcggc atcgccgcta tcgctgatat acccttcatc
     2221 acgcagtgtc agcaccagag aactgaacac cgccttgtcg aagaactccg gcgcgttgat
     2281 gccgtgcagc acggagagac gttgcgcgac ggtgcggctc tctttctcca gcgtaccgcg
     2341 gttgatcgac gggttggcac tcaacaacca gaaggtgatg gcataacgtt gcagcgtttc
     2401 gcgcgcgcct gcggccagca gctgtagcgt gcgagaatgc gccgggttga tatgcaactc
     2461 atcatcttgc agggtaatca gcccctgacg ttgcatctca tttgccagcg catcaataac
     2521 gtccggcaac tcgtcgcgat cccagcgcag gaacagctcc gctttcagca ttgggtaaag
     2581 cacattgacg tgctccatca atacgtcgcg ggagatgtgg cgatgctggg tgacgattgc
     2641 cgccatcagc gaaggcagca ccaacatatg cgcaatgttg ttgcgatagt aggtcatcag
     2701 caccgcttgc tcgcgcggca gaatgatgat gtcgccgatt gtgtctttct cgacttcaaa
     2761 cttgttcatt tgcagcgcgt gatcgataag ctcgctggcg ctggctgaag gaacggtaga
     2821 gtccgtggag taaggtacgt tgcgcatcag atccaggtag cagttgagtt gctcggttaa
     2881 ctgctcgcgg gtgagtgagc gctgacgtga cgccagaagc gcagtacagc acaggttcat
     2941 ggcgtttgcc gcgcctgcgt tgttaatgcg taccatcaga tcggcagcaa tattattgac
     3001 cgtcggcgtt aaccatgccg gacgcaccgc ttcgatggga tcgatagatt cacgccagtc
     3061 tggtacgtgc tggttaaggt aggtcatcaa cggcattggt tcaccgaagt tgacgtaacc
     3121 ctgaccgaga ttacgcagct tgcttaaacc gcgcagcatc tgcggcaggc tctctttctc
     3181 tttcgtcgcg ccgcgcagtt ctttggcgta agtacccact tccatgacgt gctcataacc
     3241 gatgtagatc ggaatcagcg taatcggacg cgtgccgcca cgcagcatcg cctgaatggt
     3301 catcgacagc gtaccggttt tcggatccag caaacgcccc gtgcgggaac gaccgccttc
     3361 cacgaagtac tcgacggaat aaccacggct gaacagttcg ccgagatact cacggaaaac
     3421 ggtggaataa agtttattgc ctttaaaagt acggcgaata aagaacgccc ccagacggcg
     3481 gaaaatcggc ccggccggcc agaagttcag gttgatccca gcggcgatat gcggcggcac
     3541 cagcccctgg tgatacagca cgtaagaaag cagcaggtag tccatgtggc tgcggtggca
     3601 aggcacatat accagctcat gaccgtcgtg ggccagctgg cgaacgcgct cggcgttatg
     3661 gacgttgatg ccctggtaaa ggcggttcca ggtgaagccc agaatacggt cagtcaggcg
     3721 aatcatctcg taagagaaat tcgccgcaat ctcttccatc agcgcaatcg cgttctgctg
     3781 cgctttttca tgggagattt ttttgctgcg cgcttcatct tctaccgctt tggcaatggc
     3841 gcgggaggcg agcagcttat taaacagatc ctgacgagca ggcagacgtg ggcccacagc
     3901 agccagacgt tgacgggcaa agtgcatacg cgccacgcgc gccagtttct gtgcgatagt
     3961 tttatccgtg ccgtgttcat ccgccatacg gcgcagcgaa actgacggcg agaaacgcac
     4021 aaaactgtcg cgaccgagcc acagtacggc gaaaaatttc tgtacgccgt taagcatacg
     4081 cagcggcggg tttacttcgc ctttttcacg ccccggcgcg cgaccaaaca tcaccgatac
     4141 tggcaccatc tgcacatcca gatttgggtt gctacggtgc aaatcgagat agtcgtggaa
     4201 cagcttaata gactcttctt tcggcgtgta ataggtgaat acacgcggcc cgccgtgaat
     4261 gaacacatag cgcggcagta gcgtgccgtc gatttccagc ggctctaacg ggtcaggcaa
     4321 gtcatgagcc agacactggg cgcgtaacgt cagcaaatct gctttcgagt tgtacggtaa
     4381 aacgtacata attggacgag aggtatccag ccccagttcc ggggcaggat ctgccggaat
     4441 agacttgctt tttaccagga tgcttaatgg taaattcagt aatttgtagt aaattcgtgg
     4501 ccagccggac ataaacgatg taaagcctct ggttaataat gcaaatgcgc ggcaaggata
     4561 gcagaaagtc atgggaaatt ctgtggtatc cgctcatgtt tcgcgcggcg ttacgcaaac
     4621 ccgaatcatc ggatttaacg gtacactgat attgacgctc ataatgtaaa aaggttcttt
     4681 caatggccaa taataccact ggattcaccc gaattatcaa agctgctggc tattcctgga
     4741 aaggtttacg cgctgcatgg atcaacgaag cggcattccg tcaggaaggc gtagcggtat
     4801 tgttggcggt ggtcatcgcc tgctggctgg atgtggacgc aattacccgc gtgctgctta
     4861 tcagctccgt gatgctggtg atgattgtgg aaatcctcaa tagcgccatc gaagcagtgg
     4921 ttgaccgaat tggctctgaa taccatgagc tttccggacg cgctaaagat atgggatccg
     4981 ccgcggtgct gattgcgatt atcgtcgccg tgataacctg gtgcatcctg ttatggtcgc
     5041 attttggata acccttccag atttcgataa atctctggtt tattgtgcag tttatggttc
     5101 caaaatcgcc ttttgctgta tatactcaca gcataactgt atatacaccc agggggcgga
     5161 atgaaagcgt taacggccag gcaacaagag gtgtttgatc tcatccgtga tcacatcagc
     5221 cagacaggta tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca
     5281 aacgcggctg aagaacatct gaaggcgctg gcacgcaaag gcgttattga aattgtttcc
     5341 ggcgcatcac gcgggattcg tctgttgcag gaagaggaag aagggttgcc gctggtaggt
     5401 cgtgtggctg ccggtgaacc gcttctggcg caacagcata ttgaaggtca ttatcaggtc
     5461 gatccttcct tgttcaagcc gaatgctgat ttcctgctgc gcgtcagcgg gatgtcgatg
     5521 aaagatatcg gcattatgga tggtgacttg ctggcagtgc ataaaactca ggatgtacgt
     5581 aacggtcagg tcgttgtcgc acgtattgat gacgaagtta ccgttaagcg cctgaaaaaa
     5641 cagggcaata aagtcgaact gttgccagaa aatagcgagt ttaaaccaat tgtcgttgac
     5701 cttcgtcagc agagcttcac cattgaaggg ctggcggttg gggttattcg caacggcgac
     5761 tggctgtaac atatctctga gaccgcgatg ccgccaggcg tcgcggtttg tttttcatct
     5821 ctcttcatca ggcttgtctg catggcattc ctcacttcat ctgataaagc actctggcat
     5881 ctcgccttac ccatgatttt ctccaatatc accgttccgt tgctgggact ggtcgatacg
     5941 gcggtaattg gtcatcttga tagcccggtt tatttgggcg gcgtggcggt tggtgcaacg
     6001 gcgaccagct ttctctttat gctgttgctg tttttacgca tgagcaccac cgggctgact
     6061 gcgcaggctt atggtgccaa aaatcctcag gcattagccc gtgcgctggt gcaaccgttg
     6121 ctgttggcgt tgggggctgg ggcgttaatt gcgctgctgc gtacaccgat tatcgatctg
     6181 gctctgcata ttgttggcgg tagtgaagca gtcctggaac aggcgcggcg ctttcttgaa
     6241 atccgctggt taagcgcacc ggcgtcgctg gcgaatctgg tattactcgg ttggttactc
     6301 ggcgtgcaat atgcccgtgc gccagtaatt ttgttagtgg tcggcaatat cctcaacatt
     6361 gtgctggatg tctggctagt gatggggctg catatgaacg tgcagggcgc ggcgctggcg
     6421 acggttattg cggaatatgc aacattgctg attggtctgc taatggtgcg taaaatcctc
     6481 aaactacgcg gaatttccgg cgaaatgctg aaaactgcct ggcgaggaaa cttccgtcgc
     6541 ttgctggcgc ttaaccgcga tatcatgctg cgttcgctgt tgttgcaact ctgtttcggc
     6601 gcgatcaccg tacttggtgc gcgactgggg agtgacatta tcgctgttaa cgcggttctg
     6661 atgacgctac tcacctttac cgcctatgcg cttgatggtt ttgcctacgc ggttgaagcg
     6721 cactccggtc aggcatacgg tgcgcgcgac ggtagccagt tgctggatgt atggcgggca
     6781 gcgtgccgcc agtcggggat tgtagcgtta ctgttttcgg tggtttattt gctggcaggg
     6841 gaacacatca ttgcgttgct gacgtcgtta acccagattc agcagctggc tgaccgctat
     6901 cttatctggc aggtgatttt gccgttggtt ggcgtctggt gttatctgct ggacggcatg
     6961 tttataggcg caacgcgtgc cgccgaaatg cgtaacagta tggcggtggc cgccgcaggt
     7021 tttgcgctga cgctccttac gctgccgtgg ctgggtaatc atggtttgtg gctggcatta
     7081 accgtctttc tggcgttgcg cgggctttct ctggcggcta tctggcggcg tcactggcgt
     7141 aacggtacct ggtttgccgc aacgtgacgg ttaaaaattc tgaataaatt atcctaagcc
     7201 aaattgctga ctacacttaa tctcacgttc agaagaaaag tgaacgtact ctcattcaca
     7261 acctaacgat gaggtcttga ttatgaataa agatgaagcc ggcggtaact ggaaacagtt
     7321 taaaggtaaa gtgaaagagc aatggggcaa actgaccgat gatgatatga cgatcattga
     7381 aggtaaacgt gatcaactgg tcggtaaaat ccaggaacgt tatggttatc agaaagatca
     7441 ggcagaaaaa gaggtcgtgg attgggaaac ccgcaatgaa tatcgctggt aattaatccc
     7501 tcctgcccga cgtgtacaag gatgtacgcc ctcttaacgc ggtttctttt tcacctgcac
     7561 agagtgatca tgctggcact gttcaggatg acgacacgct tccacttcca cgcatgccgc
     7621 acagagccca tgtgcttcaa tcacattatg ccgcagggca aaccccattt ttgccgccag
     7681 cgtatgcatg atgtcttcca cgccttctgc acattcttct ttcaccgcgc cgcagcgatc
     7741 gcaaataaac atagctgacg tatgggtggg ctggtcgaac agatggcaga gcacataact
     7801 gttggtggat tccaccttat gcacaaaacc ttgctcaagc agaaaatcca gcgcgcgata
     7861 aaccgttggc ggcttggctt gcggttcagc ttcgcgcagc aaatcaagca gatcataagc
     7921 gctgatagcg ccatcctgca gactcatcag gcgcaacact tccaggcgct gtggggtcag
     7981 gcgcacatta cgctgcgcgc agattttttc agcctgcgct aataactcct gcgttgtggt
     8041 cttttccatc gggcacctca aagtgggggt taagaatgtc cttactttac catgttccag
     8101 gaaaaacacc gagaaccctt attgttgccg taatgttgct tttctgtttt gtacgtaagg
     8161 tgttgtgttg cctggtcgta
//
LOCUS       DAPHBJ010000190         8175 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.192, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000190 DAPHBJ010000000
VERSION     DAPHBJ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.192"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..654
                     /locus_tag="RHZ76_003762"
     CDS             <1..654
                     /locus_tag="RHZ76_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HDV2814329.1"
                     /translation="ETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTI
                     NIVNGTPIHERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDS
                     VSVNQALTDKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTN
                     ENTNGLIRQYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
     gene            664..1560
                     /locus_tag="RHZ76_003763"
                     /pseudo
     CDS             664..1560
                     /locus_tag="RHZ76_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1611..2581
                     /locus_tag="RHZ76_003764"
                     /pseudo
     CDS             1611..2581
                     /locus_tag="RHZ76_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011270155.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(2806..3957)
                     /locus_tag="RHZ76_003765"
     CDS             complement(2806..3957)
                     /locus_tag="RHZ76_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDV2814330.1"
                     /translation="MAYLDPYQRPAPDRFVRRGLFITACIAALMLLWQFLPAIEAWFS
                     PREAAERTVMARGDLAADEKTTIELFEKSRASVVYITTAQLVRDVWTRNVFSVPRGTG
                     SGFIWDDAGHVVTNFHVIQGASEATVKLADGRDYQAALVGTSPAHDIAVLKIGVGFKR
                     PPAVPVGTSADLKVGQKVFAIGNPFGLDWTLTTGIVSALDRSLPGEAGGPAIDHLIQT
                     DAAINPGNSGGPLLDSAGRLIGINTAIYSPSGASAGIGFAVPVDTVMRVVPQLIKTGK
                     YIRPALGIEVDEQINARLQALTRSKGVFVLRVTPGSAAHRAGLVGVEVTAGGIVPGDR
                     VISIDGIAVDDVTTLQARLDDKNVGDVVVLLVERAGKTREMLVELQPGV"
     gene            complement(3982..4948)
                     /locus_tag="RHZ76_003766"
                     /pseudo
     CDS             complement(3982..4948)
                     /locus_tag="RHZ76_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196690.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
     gene            complement(4926..5423)
                     /gene="psiE-GI"
                     /locus_tag="RHZ76_003767"
     CDS             complement(4926..5423)
                     /gene="psiE-GI"
                     /locus_tag="RHZ76_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat resistance protein PsiE-GI"
                     /protein_id="HDV2814331.1"
                     /translation="MKSTRLNPFQVLRDQLAIMSFYERFEQVVALVLSAVIAVIIVVS
                     LFQLIAIVFTLLVLDAFNPLDHKVFQSVFGMIMTLLIAMEFKHSIVRVALRRDSIIQV
                     KTVVLIALIALSRKFVILDSDASPAKIAALASATLALGATYWLLRKRGDRAAETSEHD
                     PSSSQ"
     gene            complement(5420..7135)
                     /gene="kefB-GI"
                     /locus_tag="RHZ76_003768"
     CDS             complement(5420..7135)
                     /gene="kefB-GI"
                     /locus_tag="RHZ76_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat resistance system K+/H+ antiporter KefB-GI"
                     /protein_id="HDV2814332.1"
                     /translation="MQGLLGTTLILLATCSLAAMATAAFRVPALLGYLSVGVVLGPSV
                     IGVIAPGETLSFLSELGVALLLFMVGLEFSLGDFWLARRTVLMAGALQMIAVAPPLIL
                     LLMWLGQPGQSAALLGTAAAMSSTALVSRQLADQGELTTRHGRSAIAVLVFQDLASVP
                     LLALLAIWARGESPKIEHVLLEVFGVLLLFAAAALASRRLLHGLLGWVARRGHEESFV
                     LVSLCVVVAAAAAAHAVGVSAALGAFLAGMVLGESDFRHHMESHLRPFRDVLSGVFFV
                     TIGLQLDAAQILSAPLAVLAWLVVLVPVKILLNTLALRATRLSALDAWRTGIALGHGG
                     EFALLLLGTVLQQHLIPATVVQPMLVALVLSMALAPLLIRHHDVLARFLSRTGGVIQP
                     PQAEEVEIAAQTTRYRDHVIICGAGELGLTVSEILRHAGVAHLLLEADAQKVEAARAA
                     GAPVFHADASRPDTLLAAGLTHAHLVVLTFAHAQQALRIAQAIAEHRPALTLWVSCRS
                     TTAADAFRAMPNVRVYQQSFAAAIGLAEQVMSTLGMSTELIEGHISAMRRRLDSNRFL
                     GSSSS"
     gene            complement(7139..7579)
                     /gene="trx-GI"
                     /locus_tag="RHZ76_003769"
     CDS             complement(7139..7579)
                     /gene="trx-GI"
                     /locus_tag="RHZ76_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat resistance system thioredoxin Trx-GI"
                     /protein_id="HDV2814333.1"
                     /translation="MKNDLHLVCPHCQSINRVPTAKLSEHPNCGRCQQPLFTGEPIEL
                     TTATFSRHVERSDLPLLVDFWAPWCGPCKMMAPQFQQAAHQLEPRIRLAKVNTEAEPH
                     LATQFGIRSIPTLALFQGGREIARQAGVMGAQDIVRWTSTQVGR"
     gene            complement(7569..8168)
                     /locus_tag="RHZ76_003770"
     CDS             complement(7569..8168)
                     /locus_tag="RHZ76_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814334.1"
                     /translation="MEWQSVPGEKEAYTGLLKHLFAVLDNEVASSRQFGEFVSLDAPV
                     EPDAQDVAEAMVEEEIFEFYQHDDTLKLADIFAGSQHPDVATIAEQEELIDRTSEFAF
                     AFDLLKDMPRLWRRIFLLIRVDGLNASCVSEILLIPSKEDAVRRWLMQAEAFIAAHLE
                     EAGVSKNGNDWLQGVDWLALSVTRSEAASLWSKEANHEE"
BASE COUNT         1781 a         2463 c         2489 g         1442 t
ORIGIN      
        1 gagacaattt ataaaacgct gtactttcgt agccgtgaag cgctacacca cctgaatata
       61 cagcatctgc gacggtcgca tagccttcgc catggcaggc gtcatacccg caaaggcgaa
      121 agaggtacga ttaacatagt gaacggaaca ccaattcacg aacgttcccg aaatatcgat
      181 aacagacgct ctctggggca ttgggagggc gatttagtct caggtacaaa aaactctcat
      241 atagccacac ttgtagaccg aaaatcacgt tatacgatca tccttagact caggggcaaa
      301 gattctgtct cagtaaatca ggctcttacc gacaaattcc tgagtttacc gtcagaactc
      361 agaaaatcac tgacatggga cagaggaatg gaactggcca gacatctaga atttactgtc
      421 agcaccggcg ttaaagttta cttctgcgat cctcagagtc cttggcagcg gggaacaaat
      481 gagaacacaa atgggctaat tcggcagtac tttcctaaaa agacatgtct tgcccaatat
      541 actcaacatg aactagatct ggttgctgct cagctaaaca acagaccgag aaagacactg
      601 aagttcaaaa caccgaaaga gataattgaa aggggggttg cattgacaga ttgaatctac
      661 agcggcgtac ttcgcaaagc tgtccgactg aggtacgcct ttatccgtga caacacctgt
      721 tgctggcctg ttcgcctgct ctgtcgggtg ctggatgttc atcccagtgg tttttacgcc
      781 tggcttcagc agccgcattc acaacgccat caggcagacc tgagactgac aggacagatt
      841 aaacagttct ggctggaatc gggatgcgtc tatggttatc gcaaaatcca tctggatctg
      901 cgggacagcg ggcaacagtg cggagtgaac agagtctggc gactgatgaa acgtgtcggc
      961 ataaaggctc aggtcggata ccggagcccg cgggctcgta aaggcgaggc cagtatcgtg
     1021 tcgcccaaca ggcttcagcg acagttcaat ccggatgctc cggatgaacg ttgggtaacg
     1081 gacataacct acatcaggac ccacgaaggc tggctgtatc ttgccgtggt tgttgatctg
     1141 ttctcacgca aaattatcgg ctggtccatg caatcccgga tgacaaaaga cattgtcctg
     1201 aacgcactgc tgatggctgt atggcggcgt aacccccaaa aacaggtgct ggttcattcg
     1261 gatcagggca gtcagtacac aagccatgaa tggcagtcgt tcctgaaatc acacggcctg
     1321 gagggcagca tgagccgtcg cggtaattgc catgataatg tggttgcaga aagctttttc
     1381 cagttgttga aacgcgaacg gataaagaaa aagatctacg gaacgcggga agaagcccgc
     1441 agcgatattt ttgattacat cgaaatgttt tataacagta agcgtcggca tggttccagc
     1501 gatcagatgt caccgacaga atatgaaaac cagtattatc aacggctcgg aagtgtctaa
     1561 attatccgtg gcgattcaaa atacgctgat cccgccaccc gagttcttcc atgaagcaga
     1621 tgaccttcgc cgacgccgag tatgccggca agggcaagca gacccgcaag gaactgttcc
     1681 tgatcgagat gggtcaggtg gtgccgtgga aagggctgat tgccctgatc gaaccgcatt
     1741 atccgaaagg cgaaggtggt cgcccggcgt atcccttgat ggcgatgctg cgtatccatc
     1801 tgatgcagaa ctggttcggc tacagcgatc cggccatgga agaagcgctg tacgagacga
     1861 ccatcctacg tcagttagcc gggctgagca tggagcgtat tcctgacgaa accaccatcc
     1921 tcaacttccg tcgtctgctg gagaaacatg agctggcagc cggcatcctc tgcgtgatca
     1981 atggctacct gggggatcgc ggtctgtcgc tgcgcctggg cactatcgtc gatgccacgc
     2041 tgatccacgc gcccagttca accaagaacc aggacggcaa gcgcgacccg gaaatgcacc
     2101 agaccaagaa ggggaaccag tattactttg gcatgaaggc gcacatcggc gttgatgatg
     2161 aatcggggct ggtacacagc gtggtgggca cggcagccac cgtggcggat gtcacccagg
     2221 tcgacaagct gctgcacggc gacgagaacg tcgtctgcgc cgatgccggc tacaccggcg
     2281 tcgaaaagcg ctccgagcat gccgaccgag aagtgatctg gcaggtcgca gcacctacaa
     2341 gatgctcgat aaacgcagcg ccctgtacaa agccaagcgc aagatcgaga aggccaaggc
     2401 gcaagtgcga gcgaaggtag agcccccgtt tcgcgtgatc aagcggcagt tcggctacac
     2461 caaggtacgt ttccgagggc tggtgaggaa cactgcacaa ctggtgacgc tgtttgccct
     2521 gtcgaaccta tggatggccc gccgacattt gctggcaagc gcaggagagg tgcgcctgta
     2581 atgcggaaaa tggccgtcgc aaggtgctcg cgacggctga aagcaccgaa atgagcgggt
     2641 gatccgatcg tttttgatcg gttcaccgct ttcaaaatca gcagtggctg aagtcagccg
     2701 gaaatacagg gctacttcag accatcccta gctagggctc gtagttcggc gcaaggctga
     2761 agtcgcttgg ctgcttcgct gtgcagatcc atgacccact ttccatcaaa ctcccggttg
     2821 tagttccaca agcatctcgc gagtcttgcc ggcccgctcc actaacaaga ccacaacatc
     2881 tccaacgttc ttgtcgtcta gccgggcctg taatgtggtg acgtcgtcga cggcgatacc
     2941 gtcgatgctg ataacgcgat cgccgggcac gatgccgcct gcggtgacct cgacgccgac
     3001 gagcccggcc ctgtgcgccg ccgagcccgg cgtcacgcgc aatacgaata cgcccttact
     3061 gcgggtcagc gcctgcagac gcgcgttgat ctgctcatcc acctcgatgc ccagcgccgg
     3121 acggatgtac ttgccggtct ttatgagttg cggcaccacg cgcataacgg tatcgaccgg
     3181 caccgcaaag cctatgccgg ccgaggcgcc agacggactg tagatggcgg tattgattcc
     3241 gatcagccgc ccagccgaat cgagcagcgg gccaccggaa ttgccggggt tgatggcggc
     3301 gtcggtctgg atcaggtgat cgatggccgg gccgcccgct tctcccggca acgagcggtc
     3361 gagcgcggag acgataccag tggtgagcgt ccagtccagg ccgaaggggt tgccgatagc
     3421 gaacaccttc tgacccacct tgaggtcggc actggtgccg accggcacgg ccggcgggcg
     3481 cttgaaaccg acgccgatct tgagcacggc gatgtcgtgc gcggggctcg tccccaccag
     3541 cgcggcctgg tagtcgcggc cgtcggccag tttgacggtg gcttcggacg cgccctggat
     3601 gacgtggaag ttggtaacga cgtggccggc gtcgtcccag atgaagcccg agccagtgcc
     3661 gcgcggcacg gagaacacgt tgcgcgtcca gacatcgcgt actaattgcg cggtggtgat
     3721 gtagaccacc gaggcgcgcg atttttcgaa cagttcgatg gtggttttct cgtccgccgc
     3781 cagatcgcca cgcgccatca cggtgcgttc tgctgcctcg cgcggactga accatgcctc
     3841 gatggcgggc agaaactgcc acagcagcat gagcgcggca atgcaggcgg tgatgaagag
     3901 cccgcgccgg acgaagcggt ccggcgcagg gcgttggtac gggtcgagat aggccatgag
     3961 acgtctcctg ttgatgggca atcagcgcca cagcccgctc gggcgccagc gcggtgggcg
     4021 cgtcgccaaa gcggattccg gcaaaaaatg gggcgcgcgg agtggcaacg ctgatgctgg
     4081 cccaggcgcc aacgtcagca ggcgtgcgat gcgctcttgc gttgccggac gcgtgcgcaa
     4141 gcaggagggc tcgggattgc cccatcccgg ccatagccag gcacgccagg agcggctgac
     4201 ccgcgcgatt ttcgccagcg cggacgccag cccctgcggg tcgccagtca gttccgccgc
     4261 aaggcggtca gcgtcgaact cacgcacgcg agacaagccg agctgtgcga gcagggccag
     4321 ctggggcgat gcggccaata acaatagacc aggccaatag acctccgccg cgccaaccag
     4381 ctgcgcgggc aggctgagca ggatcgcgat ctgtcccata agggccagta ggctcgtgag
     4441 gcgactgttg gaatccgcca gccccatgac acgcagatcc tcattagcga tgtgcgcgac
     4501 ctcgtgcacg agcacacccg ccagctcgcg tgggctcagg ctgcgcagca ggccatcggt
     4561 gagggcgatc gatgcctcct gcttcgatcc cggtggcgaa ggcgttgacg acggcactgg
     4621 gcacatagtg cggcaccggc gtggcgggca aaccggcacg ggcgggcagc tcgcgcaaca
     4681 gggcccacat ctcgtgggct tcttgcgggt gcaaggcccg tatgcggtac aggcgcaagc
     4741 tcagcgccga cgcggctacc ggttccagca gcaacgcacc tgcaacggca aacagcgcga
     4801 gccacaggca gccttcccca aacggcagtc tccctgctgc ggctgcgatc tctaccatgg
     4861 tcaggaccaa cagcccggtc tgcaggcggt tgagccagcg gtgttgcaag agcgccgtgc
     4921 gcggatcact gggatgatga cgggtcatgc tcagaggtct cggcggcgcg gtcgccgcgc
     4981 ttgcgcagca gccagtaggt cgcacccaga gccagcgtgg cgcttgccag cgcggctatc
     5041 tttgcggggc tcgcgtcgga gtcgaggatc acgaacttgc gggagagtgc gatcagcgcg
     5101 atcaggacca ccgtcttgac ctggatgatg ctgtcccgac gcagcgccac gcgcacgatg
     5161 gaatgcttga actccatcgc gatcaagagc gtcatgatca tgccgaacac gctctggaat
     5221 accttgtggt ccaggggatt gaaggcatcg agaaccagca gcgtgaagac gatggcgatg
     5281 agctggaaca gcgacaccac gatgatgacg gcgatcaccg ccgacagcac gagcgcgaca
     5341 acctgctcga agcgctcgta aaaactcatg atggccaatt ggtcacgtaa gacctgaaat
     5401 gggttaaggc gtgttgattt catgacgatg aactccctag aaaacggttg ctgtcgagac
     5461 ggcggcgcat tgcgctgatg tgtccttcaa tcagctcggt cgacatgcca agcgtcgaca
     5521 tcacctgttc cgccagccca atagctgcgg caaaggattg ctgatacacg cgcacgttgg
     5581 gcatggcgcg aaatgcatcg gccgcggtcg tacttctgca tgacacccac aaggtcagcg
     5641 ccggacggtg ctcggcaatc gcctgggcga tgcgcaacgc ttgctgggca tgggcgaacg
     5701 tcagcaccac caagtgcgca tgcgtcaagc cggcagccag caaggtatcg ggccgactgg
     5761 catcggcatg gaacaccggc gcgccggcgg cacgcgcggc ctcgaccttc tgcgcgtccg
     5821 cctccagcag cagatgcgcc acgccggcgt ggcgaagaat ctcgctgact gtcaggccaa
     5881 gctcgcccgc gccgcaaatg atgacatggt ctcgatatcg cgtcgtctgc gcggcgatct
     5941 cgacttcttc agcctggggt ggctgaatga cgccgccggt gcggctcaag aaccgggcaa
     6001 gtacatcgtg atggcggatc agtagcggtg ccagggccat gctgagcacc agcgcgacca
     6061 gcatgggttg gacgacggtc gcggggatca gatgctgctg caagaccgtg cccaacaaca
     6121 gcagggcgaa ctcgccgccg tgccccaacg ctatgcccgt gcgccaggca tcgagggcgg
     6181 acaggcgcgt cgcccgcaag gccagcgtat tgagcaggat cttgaccggt accagcacca
     6241 ccagccacgc cagcaccgcc agcggtgccg aaagaatctg tgctgcgtcc agttgcaggc
     6301 cgatggtcac gaagaacacg cccgacaaca catcgcgaaa cggcctgagg tggctttcca
     6361 tgtggtgccg gaagtcgctc tcgcccagca ccatcccggc gaggaaggcc cccagggcgg
     6421 cggatacgcc gaccgcgtgc gcagcggcgg cggcagccac caccacgcac aaggagacga
     6481 gcacgaacga ttcctcgtgg ccccgccgtg ccacccagcc cagcaggcca tgcaacagac
     6541 ggcgcgaggc gagggccgct gccgcgaaca gcagcagcac accgaatact tcgagcagca
     6601 catgctcgat cttcggcgac tcgccgcgcg cccagatcgc cagcaaggcc agcaggggca
     6661 cgctggccag gtcctgaaag accagcacgg cgatggcact gcgtccgtgg cgggtggtga
     6721 gctcaccttg gtcggccagt tgtcggctga ccagcgccgt ggacgacatg gccgccgcag
     6781 tgccgagcag cgcggcgctc tggccgggct gccccagcca catcaatagc aggatcagcg
     6841 gcggggccac ggcgatcatc tgcagagcgc ccgccatcag caccgtcctg cgggcgagcc
     6901 agaagtctcc gagggagaat tccagtccga ccatgaacaa cagcagcgcc acgcccagct
     6961 cggacagaaa gctcagcgtc tcccccggcg cgatcacgcc gatgactgag gggcccagta
     7021 caacgccgac agagagatac cccagcaagg cgggcactct gaacgccgcc gtcgccatcg
     7081 ccgccaggct gcaagtcgcc agcaggatta gtgtagtgcc gagcaagccc tgcatcggtc
     7141 agcgtcccac ttgcgtcgac gtccagcgca cgatgtcctg cgcgcccatc acgccggcct
     7201 gacgggcgat ctcccgccca ccctggaaca gggcgagcgt cgggatgctg cggataccga
     7261 actgcgtggc caggtggggc tcagcctcgg tattcacctt cgccagccgg atcctcggtt
     7321 cgagctggtg cgccgcttgc tggaactgtg gcgccatcat cttgcacggc ccgcaccatg
     7381 gcgcccagaa atcgaccagc agcggcagat cgctgcgctc cacgtggcgc gagaacgtcg
     7441 ccgtggtcaa ctcgatgggc tcgcccgtga acaagggctg ctggcagcgg ccacagttgg
     7501 gatgttccga tagcttcgca gtcggtacgc ggttgatgga ctggcagtgc gggcagacga
     7561 ggtgaagatc attcttcatg gttcgcctcc ttggaccaca gtgaggctgc ctcgcttcgg
     7621 gtcacagaca atgccaacca atcaacgcct tggagccaat cgttcccgtt tttgcttact
     7681 ccggcctctt ccagatgagc agcgatgaat gcttcggcct gcatgagcca ccttcggaca
     7741 gcatcttcct tactaggaat cagcaggatt tcagagacgc agctggcatt cagcccgtcc
     7801 acacggataa gcagaaaaat gcgccgccac aaacgcggca tgtccttcag caggtcaaat
     7861 gcaaaagcga actcgctcgt ccgatcaatc agctcctcct gttccgcgat cgttgcgaca
     7921 tcgggatgct gactgccagc gaaaatatcg gccagcttga gtgtgtcatc gtgctgataa
     7981 aattcgaaga tttcctcttc caccatggcc tcggccacgt cttgggcatc cggttcgacc
     8041 ggcgcgtcca gagaaacgaa ttcgccaaat tgccggctgc ttgccacttc gttatccagg
     8101 acggcgaaca gatgcttcaa aagaccggtg taagcctctt tttcccccgg cacggattgc
     8161 cattccacct tagct
//
LOCUS       DAPHBJ010000191         8174 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.193, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000191 DAPHBJ010000000
VERSION     DAPHBJ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.193"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            49..546
                     /gene="ftnA"
                     /locus_tag="RHZ76_003771"
     CDS             49..546
                     /gene="ftnA"
                     /locus_tag="RHZ76_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HDV2814335.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(584..823)
                     /locus_tag="RHZ76_003772"
     CDS             complement(584..823)
                     /locus_tag="RHZ76_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HDV2814336.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            1014..2225
                     /gene="tyrP"
                     /locus_tag="RHZ76_003773"
     CDS             1014..2225
                     /gene="tyrP"
                     /locus_tag="RHZ76_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HDV2814337.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2287..2952)
                     /gene="yecA"
                     /locus_tag="RHZ76_003774"
     CDS             complement(2287..2952)
                     /gene="yecA"
                     /locus_tag="RHZ76_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HDV2814338.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3148..3234)
                     /locus_tag="RHZ76_003775"
     tRNA            complement(3148..3234)
                     /locus_tag="RHZ76_003775"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3198..3200),aa:Leu,seq:taa)
     gene            complement(3247..3320)
                     /locus_tag="RHZ76_003776"
     tRNA            complement(3247..3320)
                     /locus_tag="RHZ76_003776"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3286..3288),aa:Cys,seq:gca)
     gene            complement(3375..3450)
                     /locus_tag="RHZ76_003777"
     tRNA            complement(3375..3450)
                     /locus_tag="RHZ76_003777"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3415..3417),aa:Gly,seq:gcc)
     gene            complement(3602..4150)
                     /gene="pgsA"
                     /locus_tag="RHZ76_003778"
     CDS             complement(3602..4150)
                     /gene="pgsA"
                     /locus_tag="RHZ76_003778"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDV2814339.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4207..6039)
                     /gene="uvrC"
                     /locus_tag="RHZ76_003779"
     CDS             complement(4207..6039)
                     /gene="uvrC"
                     /locus_tag="RHZ76_003779"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDV2814340.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     HNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVIAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6036..6692)
                     /gene="uvrY"
                     /locus_tag="RHZ76_003780"
     CDS             complement(6036..6692)
                     /gene="uvrY"
                     /locus_tag="RHZ76_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HDV2814341.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAVGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            6988..7164
                     /locus_tag="RHZ76_003781"
                     /pseudo
     CDS             6988..7164
                     /locus_tag="RHZ76_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7151..7375
                     /locus_tag="RHZ76_003782"
     CDS             7151..7375
                     /locus_tag="RHZ76_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HDV2814342.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7443..8165)
                     /gene="sdiA"
                     /locus_tag="RHZ76_003783"
     CDS             complement(7443..8165)
                     /gene="sdiA"
                     /locus_tag="RHZ76_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HDV2814343.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQLLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLIWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         2032 a         2094 c         1911 g         2137 t
ORIGIN      
        1 attatagtga cgccacagca acaaatataa cctttgtgga gcactatcat gctgaaacca
       61 gaaatgattg aaaaacttaa tgagcagatg aacctggaac tgtactcttc actgctttat
      121 cagcaaatga gcgcctggtg cagctatcat accttcgaag gtgctgccgc gttcctgcgc
      181 cgtcacgccc aggaagagat gacgcatatg cagcgtctgt ttgattacct gactgatacc
      241 ggcaatttac cgcgtattaa taccgttgaa tctccgtttg ctgaatattc ctcacttgat
      301 gaattattcc aggaaaccta taaacacgaa caattaatca cccagaaaat taacgaactg
      361 gctcatgctg caatgaccaa tcaggactac ccaacattta atttcctgca atggtatgtt
      421 tctgagcagc atgaagaaga gaaactgttc aaatcgatta ttgataaatt aagcctggca
      481 ggcaaaagcg gcgaaggtct gtattttatc gacaaagaac tctctaccct cgacacacaa
      541 aactaatgct ctccggcggc aggtttactg ccgccgtttc aaatcagtga cggcaaatct
      601 tactctgatc ggtcgaaaaa ccgtctttcg aaggtataaa tttgcctttt gctgccagaa
      661 acgctaccag ctctcccgct gtcatccctt ctgccgagca ggtgtgaaaa cgtgcctgtt
      721 caccaaaacg cgctttaatc gcagcttcca gactggcatg cgtatattgc tcgcctgatt
      781 caatcatcat atttaacact tcatgaccgt gaatagagtc catcgtccct cctcaaaaaa
      841 agcctagcgt agcgattgcc gcttatgaag actttgcgcc agcgcaggac tgaatgcttt
      901 ttattgtaca tttatattta caccatatgt aacgtcggtt tgacgaagca gccgttatgc
      961 cttaacctgc gccgcagata tcactcataa agatcgtcag gacagaagaa agcgtgaaaa
     1021 acagaaccct gggaagtgtt tttatcgtgg cgggaaccac aattggcgca ggcatgctgg
     1081 caatgccgct ggctgcggcc ggtgttggtt ttagcgttac gttaatcttg ttgattgggc
     1141 tttgggcgtt gatgtgctac acggcgctat tactgctgga ggtgtaccag catgttccgg
     1201 cagataccgg tctgggcacg ctggcaaaac gctatctggg acgctacggt caatggctga
     1261 cgggcttcag tatgatgttc ttaatgtatg ctctgactgc ggcatacatc agcggtgccg
     1321 gtgaattgtt ggcctccagc atcagcgact ggacaggtat ttcaatgtcg gcaaccgctg
     1381 gcgtgctgtt gttcactttt gttgccggtg gcgtggtttg tgttggaaca tcgctggtcg
     1441 atttatttaa ccgttttctg ttcagcgcca aaattatttt tctggtggtg atgctggtat
     1501 tgctactacc gcatattcac aaagtgaatc ttttaaccct gccgttgcaa caggggctgg
     1561 ctctgtcagc aatcccggtg atttttacct catttggttt tcacggtagc gtgccgagta
     1621 ttgtcagcta tatggatggc aacgttcgta agctacgctg ggtgtttata accggtagtg
     1681 cgatccccct ggtggcatat attttctggc aggtggcaac gcttggcagc attgattcaa
     1741 caacctttat gggattgctg gctaatcatg ctggattaaa cgggctgtta caggcgttac
     1801 gcgaaatggt ggcctctccg catgttgagc tggcagtgca tttatttgct gatttagccc
     1861 tcgccacgtc atttctcggc gttgcgttag gcttatttga ttatctggct gatttatttc
     1921 agcgttcaaa taccgttggt ggacggttgc aaactggtgc aattaccttt ctgccgccgt
     1981 tggcgtttgc actgttttat ccacgaggat ttgtgatggc gctgggttac gccggtgtgg
     2041 cgctggcggt actggcattg attatccctt cgctgttgac ctggcaaagc agaaagcaca
     2101 atcctcaggc gggttaccgg gtgaaaggtg gtcgtccggc gctggtggtg gtgtttctct
     2161 gtggtattgc tgtgattggc gtgcaatttt tgattgcggc agggttgtta ccagaagtgg
     2221 ggtgatcaga tagcctcaaa ttccttattg ggtgccagaa ttaacgctga cacccaattt
     2281 gacctcttaa tgcaggcagc actgcttaaa tttcttacca ctaccgcacg ggcaaggatc
     2341 gttacgcccc ggtttctctt ctgctttgat cggttgctga acagcttttt cctgcggatg
     2401 cgccatccag tacgcatgta gatcaagcgc cgccagtcga atggcatcta cgctctcttc
     2461 aaacgcttct ggcgacattt tctctacccg ctcgaagttt tcctcagtac cgtgcagcgc
     2521 aatcgcctcc agcgctggtt ttaacgaatc gggcaacgtt gaccagtcag aaagtgccac
     2581 gccccgcata tagccaaagc accactcctc aacaatcgtc agctcgctgc catcaacttc
     2641 tcgcaagccg aataacggct caaactgctc cgggaattcg ttcagacgct ctgcggtatc
     2701 ggccatatgt tgaaaagcca gattcataaa gcgcgtcatc tctttctctg acgcccagcg
     2761 cggcacatag tcagccccac cccacacggc aaccagccac tgtgccggtt caatctcttg
     2821 cggagaactc aacaccgccg tcaataaacc gtccagctcc gccacatcaa ggatggcgtg
     2881 gtcagtgttg tatttggtca gaatatcgtc cagccattcc aactcacttt cgtttaacgg
     2941 tcccgttttc atacgctttt ccttgtggat ctcaactcgc cagcacctat cttacatgcc
     3001 ggtccgtatc agagatactt tttgagtggc tttgctggtg attaaaaatt aaggagggtg
     3061 taacgacaag ttgcaggcac aaaaaaacca cccgaaggtg gtttcacgac actgcttatt
     3121 gctttgattt tattcttatc tttcccatgg tacccggagc gggacttgaa cccgcacagc
     3181 gcgaacgccg agggatttta aatcccttgt gtctaccgat tccaccatcc gggctcggga
     3241 agaaagtgga ggcgcgttcc ggagtcgaac cggactagac ggatttgcaa tccgctacat
     3301 aaccgctttg ttaacgcgcc aaattcttca ggcctttcag ccagacatcc gcttgaggcc
     3361 gatgtctttt aaactggagc gggaaacgag actcgaactc gcgaccccga ccttggcaag
     3421 gtcgtgctct accaactgag ctattcccgc attcatcaag caatcagtta atcacttgat
     3481 tttattatcg tctggcaatc agtgccgccg ttcgatgcgt tgcattctac ttacctggcg
     3541 cgatgagtca acgatatttt tcaccacttt tgatcgtttg ctgaaaatta cgccgaaacg
     3601 atcactgatc aagcaaatct gcacgcgcag cgctcaaata ttgcaacatt gaccacagag
     3661 tcagtaccgc agccacaaag aaaagtgcaa taccggcgta ctcaacccaa atgttcggac
     3721 gccacagcag ccaggccaac gccaccatct gggcagtggt tttcactttc ccaatccagg
     3781 agacagccac gctactgcgt ttacccaact ccgccatcca ttcgcgtagc gcagaaataa
     3841 taatttcacg ggcgatcatc gttgccgccg gtagggtcac ccaccagctg tgataatgct
     3901 cggttaccag caccatggcg atagccacga gaactttatc tgctacaggg tcaaggaaag
     3961 caccaaaccg ggtactctgg ttccagcggc gtgccagaaa accatcgaac cagtcagtca
     4021 ccgccgcaac gcagaaaatg agcgcggcgg caaacggcga ccaggtgaca ggcagataaa
     4081 agaccaatac aaagaatggg ataaggatga cacggaacag tgtaagcaac gtagggatat
     4141 taaattgcat aatgacgggt aactatctgt tgtcagtaag attaccccta tgttgctaca
     4201 gagacatcaa tgtttcaacg accagaagat cttttctgcc agaccttgcg aaatacccgg
     4261 cacttttgca atttcctcga cgctggcgtt acgtaaacct tgcaaaccgc ccatatattt
     4321 caacaacatt tgccgacgtt ttggcccgac gccttcaatg gtttccaggg agctggtatt
     4381 tttgaccttc gcccgttttt tacggtgccc gccaatcgcg tgatcgtgtg attcatcgcg
     4441 aatatgctgg ataacatgca gcgcgggaga atctggcggc aaactgaatc cctcaccttc
     4501 cggctcaaag aacagcgttt ccagcccagc cttacgatct gctcctttgg caacgccaag
     4561 tagcagcgga tgatttttat cccatgagac atccagttcg gcgatgacat ttttcgcctg
     4621 cgcaagctgg cctttgccgc cgtcgataag gatcacatcc gggatcttac tgtcgtcaat
     4681 ggctttacca taacgccgac gcagcacctg attcatcgcc gcataatcat cgcccggcgt
     4741 gatgcctgta atgttatagc gccgatactc cgcacgcagc gggccgttag catcaaacac
     4801 cacacaggaa gcgacggttt gttcacccat ggtatggctg atgtcaaagc actccatccg
     4861 cttcacttcc ggcaatttca acacgctggc aagcgcggtc agtcgctggt gaacggtaga
     4921 ttgctgcgaa agtttgctgg ttaaggccgt cgccgcattg gtgcgcgcga gtttcagata
     4981 acgcgcccta tcgccgcgag gtttggtttg aacattaatc ttgcgtcccg ccagttctga
     5041 aagggaatcg gcgagaagcg ttttatcgct aagattaaaa tcgagcagga tctcacccgg
     5101 taaggtgcgc atctggctgc cttgtaaata gaactgtcct acgaaggttt ccaccacctc
     5161 gctcagttcc gtaccgccag gcactttcgg gaaatagctg cggctgccaa gcactttgcc
     5221 ctgacgaatg aacaatacgt ggacacaagc catgcccgca tcgaacgcca caccaataac
     5281 gtcaaggtcg tcgccggtat tggaaacaaa ctgtttttcg gtgacgcgtc gcaccgcctg
     5341 aatttggtcg cgaatacgtg ccgcttcttc aaactccaga ttgtgactgg cagtttccat
     5401 acggctaatc agttgcgtaa gcacctgatc atctttgcca gacaaaaaca ggcgcacata
     5461 ctcgacctgc tgagcgtatt cttcttcact caccagtcct tcaacgcacg gtcccagaca
     5521 gcgccctatc tgatattgca gacatggacg cgagcgattg cgataaacac tattttcgca
     5581 ctggcgaatg gggaaaatct tttgcagtag cgccagtgtt tcacgtacgg catagccatt
     5641 cgggaacggg ccgaaatatt cacctttggc atgcttcgca ccacgatgca tcgccagacg
     5701 cgggtgggta tcgccactca ggaagataaa aggatatgat ttatcatcgc gtagcaaaac
     5761 attgtaacgc ggctgataga gtttgatgta gttgtgttcc agcaacagcg cttcggtttc
     5821 tgtatgagta accgttacat caatttgctg gatctgggcg accagcgctt cggttttgcg
     5881 cgaagcgagg ttgctacgga aatagctgga aagccgtttt ttcaggtctt tcgctttgcc
     5941 gacatagata accgtaccac cagcatcgta catgcgataa acgccaggct ggctggttac
     6001 ggtttttaaa aacgcttttg cgtcaaactg atcactcact gacttgataa tgtctccgca
     6061 ttacacagac catggcgaat tgccaggtga gtcagctcaa catcgccatg aatgtttagt
     6121 ttactgaaca tacgatagcg gtagctgttc accgttttcg gactgagatt gagctgttct
     6181 gagatctcat tgaccttctg gcccttggtg atcatcagca taatctgcaa ttcacgttca
     6241 gacaaactgg caaatgggct ttctgttttt tctggttcga tctggcttaa cgccatttgt
     6301 tgagcgatat cagaagcaat gtaacgctgc cctgaataga cagaacgaat cgcactcacg
     6361 acttcctgcg gagccgcgcc tttgctgagg tagcccacag caccggcctg catgactttc
     6421 gctggtaaag ggttttctgt atggacggta agcatgatga ttttgacatc agctgtggaa
     6481 cgcgcgattt tacgcgtcgc ctcaagaccg ccaatgcccg gcatactcat gtccattagc
     6541 accacgtcaa cggcatttgc ccggcaccac ttaacggcgt cttcaccgca cgatgcctca
     6601 ccgacgactt ttataccctt tatatcttcc agaatgcgtc gtatccctgc gcgcaccagt
     6661 tcgtggtcat caacaagtag aacgttgatc aaaggaatat ctccagaaat agggataacg
     6721 ctactgatag ttagtcattc gtatattagc ggtttttatt gcaactttga aacgttaaaa
     6781 atggtgggta ttcgattttt ctctcgtttt tgtaaattcg attgtccaca gttatggtca
     6841 cgcccgtcac caacggcttc cgggatcgtt tcacagtaag cccggcgatg aaatgtgaca
     6901 aaaattgact ttattcagca aaaatgaaaa tcagcccgct gaatatgtaa cattaattaa
     6961 cccgaacaat acgggcaaaa aacattcatg ataaaaatat ttattggtca ttatattaac
     7021 gtattttata gtactgttga tatcacgctc aaaaaataac cactgctatt tttagcaaag
     7081 cttatggtat actccgccgc cttaacattt ttcaccgcaa attttcattg caacatgacg
     7141 aggaaaataa atgagtacgc ctgatttttc tactgccgag aataatcaag aactggcaaa
     7201 tgaagtctcc tgcctgaaag cgatgctgac gctgatgctg caggcgatgg gacaagctga
     7261 cgcgggccgc gtgatgttaa agatggaaaa acagcttgcg ctgatcgaag acgaaaccca
     7321 ggctgcagta ttttccaaaa cggttaagca aattaaacaa gcctaccgtc agtaatataa
     7381 aaccggctga tagcgtgcct ttcagccggt ttttgcatct ggcacgcagg acagaaaaga
     7441 gatcaaatta agccagtagc ggccgcgtaa caggcaacct gggtcttatt tggtgcatta
     7501 atttttttct gcatgttttt ctggtggaaa ttgaccgtat tctccgagat tgacaaaatc
     7561 atcgctatct ctgctgatgt cttcccttcc gccgtccacc tcagaatttc tttttcgcgc
     7621 ttgctgaaat tcatctctgg cgtcatcact atttcatcat ttaaccgcat cagagccatc
     7681 agactttcgc gcaccagtaa ctgcattttt aattgcagct catcactaag aatgggtatt
     7741 tcgcgcgcgc tgcaacggga aaaggacaaa aagcccagcg cccggtttgg cagcattaaa
     7801 tactgagtga caccgcggcg taaaccatgt gcgcgcgcgg cttcccataa cggctgtgct
     7861 tcgctgaata agtcatcatt ccatattaaa tggccctgac taaagttttc agggttcagc
     7921 accggatcaa ttgcgagaaa gttttttgcc tgataataac taacccacgc ctcagggtaa
     7981 ttggtgtaaa aagccacttt aggtcgcgtg aatggtaccg ggtggcgaac acataacgaa
     8041 tagtaatcgt actccagcag ctgagcctga agctctattt catggtagac ctcttctgcg
     8101 gcctccatcc tctgaaaacg caacagcatc gtgcgacgcc agctgaaaaa atccgtatcc
     8161 tgcatagtaa accg
//
LOCUS       DAPHBJ010000192         8161 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.194, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000192 DAPHBJ010000000
VERSION     DAPHBJ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.194"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            111..1088
                     /gene="csiD"
                     /locus_tag="RHZ76_003784"
     CDS             111..1088
                     /gene="csiD"
                     /locus_tag="RHZ76_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HDV2814344.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1107..2375
                     /gene="lhgO"
                     /locus_tag="RHZ76_003785"
     CDS             1107..2375
                     /gene="lhgO"
                     /locus_tag="RHZ76_003785"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HDV2814345.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYHKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2398..3846
                     /gene="gabD"
                     /locus_tag="RHZ76_003786"
     CDS             2398..3846
                     /gene="gabD"
                     /locus_tag="RHZ76_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDV2814346.1"
                     /translation="MKLNDSKLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLLMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALSSKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLEKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            3860..5140
                     /gene="gabT"
                     /locus_tag="RHZ76_003787"
     CDS             3860..5140
                     /gene="gabT"
                     /locus_tag="RHZ76_003787"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HDV2814347.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGNFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYASS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            5378..6778
                     /gene="gabP"
                     /locus_tag="RHZ76_003788"
     CDS             5378..6778
                     /gene="gabP"
                     /locus_tag="RHZ76_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HDV2814348.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            6799..7461
                     /gene="csiR"
                     /locus_tag="RHZ76_003789"
     CDS             6799..7461
                     /gene="csiR"
                     /locus_tag="RHZ76_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HDV2814349.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7462..7911)
                     /gene="kbp"
                     /locus_tag="RHZ76_003790"
     CDS             complement(7462..7911)
                     /gene="kbp"
                     /locus_tag="RHZ76_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HDV2814350.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(7995..8153)
                     /locus_tag="RHZ76_003791"
     CDS             complement(7995..8153)
                     /locus_tag="RHZ76_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HDV2814351.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         1811 a         2152 c         2326 g         1872 t
ORIGIN      
        1 acaatatgtc gcttttgtgc gcatttttca gaaatgtaga tatttttaga ttatggctac
       61 gaaatgagca tcgccatgtc accctacatc tcataagagg atcgcttctg atgaatgcac
      121 tgaccgccgt acacaataac gctgtcgatt caggccagga ctatagcgga ttcactctca
      181 tcccgtcggc gcaatccccg cgtctgctgg aactcacctt caccgaacag acgaccaaac
      241 agtttctcga acaggttgcc gaatggcccg tgcaggcgct ggagtacaaa tcgtttctgc
      301 gttttcgggt aggcaaaatt cttgacgatc tgtgtgcgaa tcagctgcaa ccattgctgt
      361 tgaagaccct gctaaaccgt gctgaaggtg cgctgttgat caatgcggtg ggtatcgatg
      421 atgtggcgca ggcagatgag atggtgaagc tggcaacggc ggtagcgcat ctgattggtc
      481 gctcaaattt cgatgcgatg agcggtcagt attacgcgcg tttcgtggtg aaaaatgtcg
      541 ataactcaga cagctatctg cgtcagccgc accgcgtgat ggaactgcac aacgacggca
      601 cgtatgtcga agagatcacc gattacgtgc tgatgatgaa aatcgatgag caaaacatgc
      661 agggtggaaa ttcgttgctg ctgcatctcg atgactggga acatctggac cactatttcc
      721 gccacccgct ggcgcgtcgc ccaatgcgct ttgccgcgcc gccgagtaaa aacgtcagca
      781 aagatgtttt ccatccggtg ttcgacgttg atcaacaggg tcgcccggtg atgcgttata
      841 tcgaccagtt cgtccagcca aaagacttcg aagaaggcgt gtggttgagc gagctttcgg
      901 acgccattga aaccagcaaa ggcattcttt ctgtgcccgt tcccgttggc aaattcctgt
      961 tgattaacaa cctgttctgg ctgcacggtc gcgaccgctt tactccgcac ccggatctgc
     1021 gccgtgaact gatgcgtcag cgtggctatt tcgcttacgc cactcaccac taccagacgc
     1081 atcagtagcg caaaggaatt gagcggatgt atgattttgt gattattggc ggtggcatca
     1141 tcggcatgtc gaccgccatg caactgattg atgtctaccc ggacgcccgc attgcgttgc
     1201 tggaaaaaga gtccggcccg gcctgtcacc agacgggcca caacagcggc gtgatccatg
     1261 ccggggttta ttacacgccc ggtagcctga aggcacagtt ttgcctggcg ggaaaccgcg
     1321 ccactaaagc cttttgcgat caaaacggca ttcgctatga caactgcggc aagatgctgg
     1381 tcgccacctc tgaactcgaa atggaacgga tgcgcgcgtt gtgggaacgc acggcggcaa
     1441 acggtatcga gcgcgagtgg ttaaacgccg atgaactgcg cgagcgcgaa ccgaatatca
     1501 ccgggcttgg cggtattttt gtgccgtcca gcggcattgt cagctaccgc gaagtaacgg
     1561 cggcgatggc aaaaattttc caggccagag gcggcgagat tatctataac gccgaagtca
     1621 gcggcctcag tgagcataaa aacggcgtgg tgatacgtac ccgccagggc ggcgaatatg
     1681 aagcatcgac gctgattagc tgttccgggc tgatggctga ccggctggtg aaaatgctcg
     1741 gactcgaacc gggctttatc atctgcccgt tccgtggtga gtatttccgc cttgcgccgg
     1801 agcataacca gattgttaac cacctgattt accccattcc cgaccccgcg atgccatttt
     1861 tgggcgtgca tctcacccga atgatcgatg gcagcgtgac cgtcgggcca aacgcggtgc
     1921 tggcttttaa acgcgaaggc taccacaagc gcgacttctc gtttagcgac acgctggaaa
     1981 ttttgggctc gtcggggatt cgccgggtgc tgcaaaacca tctacgctca ggactgggcg
     2041 agatgaaaaa ctcgctgtgc aaaagcggct atctgcggct ggtgcaaaag tattgtcccc
     2101 ggctttcgtt aagcgatctc cagccctggc ccgccggtgt gcgggcgcag gcggtatcgc
     2161 cggacggcaa gctgattgac gattttctgt ttgtcaccac cccgcgcacg atccacacct
     2221 gcaatgcgcc ctccccggca gcgacatcag caattcctat tggtgcgcat attgtcagca
     2281 aggtacaaac gctgttggca agccagagta accccggacg cacgctgcga gcggcacgta
     2341 gtgtggatgc cttacacgcc gcatttaatc aataaccttt gaaaacagga cgtaacaatg
     2401 aaacttaacg acagtaaatt attccgccag caggcgttga ttaacgggga atggctggac
     2461 gccaacaatg gcgaggtcat cgatgtcacc aatccggcga acggcgacaa gctgggtagc
     2521 gtacccaaaa tgggcgctga tgaaacccgc gccgctatcg acgccgccaa ccgcgctctg
     2581 cccgcctggc gtgcgctcac cgccaaagaa cgcgccaaca ttctgcgcaa ctggttcaat
     2641 ttgttgatgg agcatcagga cgatttagcg cgcctgatga ccctagaaca gggcaaaccg
     2701 ctggctgaag cgaaaggcga aattagctac gccgcctcct ttattgagtg gtttgctgaa
     2761 gaaggcaaac gcatttatgg cgacaccatt cctggtcatc aggccgataa gcgcctgatt
     2821 gttatcaagc agccgattgg cgtcaccgca gctatcacgc cgtggaactt cccggcggcg
     2881 atgattaccc gcaaagccgg tccggcgctg gcggcaggct gcaccatggt gctgaagcct
     2941 gccagtcaga cgccgttctc tgcgctggcg ctggcggagc tggcgattcg cgcgggcatt
     3001 ccggctgggg tatttaacgt ggtcaccggt tcggcgggcg cggtgggtaa cgaactgacc
     3061 agcaacccgc tggtgcgcaa gctgtcgttt accggttcga ccgaaattgg ccgccagtta
     3121 atggaacagt gcgcgaaaga catcaaaaaa gtgtcgctgg agctgggcgg caacgcgccg
     3181 tttatcgtct ttgacgatgc cgacctcgac aaagccgtgg aaggtgcgct gtcctcgaaa
     3241 ttccgcaacg ccgggcaaac ctgcgtctgc gccaaccgtt tatacgtgca ggacggcgtg
     3301 tatgaccgct ttgccgaaaa attgcagcag gcggtgagca aattgcacat cggcgacggg
     3361 ctggagaaag gcgtcaccat cgggccgctg atcgatgaaa aagcggtagc aaaagtggaa
     3421 gagcatattg ccgatgcgct ggagaaaggt gcgcgtgtgg tttgcggcgg taaagcggat
     3481 gaacgcggcg gcaacttctt ccagccgacc attctggtgg acgttccagc caacgccaaa
     3541 gtgtcgaaag aagagacgtt cggccccctc gccccactgt tccgttttaa agatgaagcc
     3601 gatgtgattg cgcaagccaa tgacaccgag tttggccttg ccgcctattt ctacgcccgt
     3661 gatttaagcc gcgtcttccg cgtgggcgaa gcgctggagt acggcatcgt cggcatcaat
     3721 accggcatca tttccaatga agtggccccg ttcggcggca ttaaagcctc gggtctgggt
     3781 cgtgaaggtt cgaagtatgg catcgaagat tacttagaaa tcaaatatat gtgcatcggt
     3841 ctttaactgg agaacgcgaa tgagcagcaa taaagagtta atgcagcgcc gcagtcaggc
     3901 gattccccgt ggtgttgggc aaattcaccc gattttcgct gaccgcgcgg aaaactgccg
     3961 ggtgtgggac gttgaaggcc gtgagtatct tgatttcgcg ggcgggattg cggtgctcaa
     4021 taccgggcac ttgcatccga aagtggtggc tgcggtggaa gcgcagttga aaaaactgtc
     4081 gcacacctgc ttccaggtgc tggcctacga gccgtatctg gagctgtgcg agattatgaa
     4141 tcagaaggtg ccaggcaatt ttgccaagaa aacgctgctg gtcaccacag gttctgaagc
     4201 ggtggaaaac gcggtgaaaa tcgcccgcgc cgccaccaaa cgtagcggca ccatcgcttt
     4261 tagcggcgcg tatcacgggc gcacgcatta cacgctggcg ctgaccggca aggtgaatcc
     4321 gtactctgcg ggcatggggc tgatgccggg tcatgtttat cgcgcgcttt atccttgccc
     4381 gctgcacggc ataagcgagg atgacgctat cgccagcatc caccggatct tcaaaaatga
     4441 tgccgcgccg gaagatatcg ccgccatcgt gattgagccg gttcagggcg aaggcggttt
     4501 ctacgcctcg tcgccagcct ttatgcagcg tttacgcgct ctgtgtgacg agcacgggat
     4561 catgctgatt gccgatgaag tgcagagcgg cgcggggcgt accggcacgc tgtttgcgat
     4621 ggagcagatg ggcgttgcgc cggatcttac cacctttgcg aaatcgatcg cgggcggctt
     4681 cccgctggcg ggcgtcaccg ggcgcgcgga agtaatggat gccgtcgctc caggcggtct
     4741 gggcggcacc tatgcgggta acccgattgc ctgcgtggct gcgctggaag tgttgaaggt
     4801 gtttgagcag gaaaatctgc tgcaaaaagc caacgatctg gggcagaagt tgaaagacgg
     4861 attgctggcg atagccgaaa aacacccgga gatcggcgac gtacgcgggc tgggggcgat
     4921 gatcgccatt gagctgtttg aagacggcga tcacaacaag ccggacgcca aactcaccgc
     4981 cgagatcgtg gctcgcgccc gcgataaagg cctgattctt ctctcctgcg gcccgtatta
     5041 caacgtgctg cgcatccttg taccgctcac cattgaagac gctcagatcc gtcagggtct
     5101 ggagatcatc agccagtgtt ttgatgaggc gaagcagtag cgccgctcct atgccggagg
     5161 cgacgctgcg cgtcttgtcc ggcctacggg gatccaggtc ggataaggcg tttacgccgc
     5221 atccgacaat ctgtacgtga acaggaagaa atctatgttg gccgggtaag gcggagccgc
     5281 tctccggcaa aaagaatcaa taacaattat acgcgtgacc cggcgcggga aatgtcgggg
     5341 cgctctccca agtgacacac tttcgagagg attcaggatg gggcaatcat cgcaaccaca
     5401 tgagttaggc ggcgggctga agtcacgcca cgtcaccatg ttgtctattg ccggtgttat
     5461 cggcgcaagt ctgtttgtcg gttccagcgt cgccatcgcc gaagcgggcc cggcggtatt
     5521 actggcctat ctgttcgccg ggctactggt ggttatgatt atgcggatgt tggcggaaat
     5581 ggcggttgcc acgcccgata ccggttcgtt ttccacctat gccgataaag ccattggtcg
     5641 ctgggcgggc tataccatcg gctggttgta ctggtggttt tgggtactgg ttatcccgct
     5701 ggaagccaac atcgccgcca tgatcctgca ctcatgggtt ccaggcattc ccatctggtt
     5761 attttccctc gtcattaccc tcgccttaac tggcagtaac ttattaagcg ttaaaaacta
     5821 cggcgaattt gagttctggc tggcgctgtg caaagtcatc gctatcctgg cctttatttt
     5881 ccttggtgca gttgcaatta gcggttttta cccgtatgcc gaagtgagcg ggatctcaag
     5941 attgtgggat agcggcggct ttatgcccaa cggtttcggt gcggtattaa gcgcgatgtt
     6001 gatcaccatg ttctcgttta tgggcgcaga aattgtcacc attgccgccg cggaatccga
     6061 cacgccggaa aaacatattg tccgcgccac caactcggtt atctggcgta tttctatctt
     6121 ctatttatgt tctatttttg tcgtcgtggc attaattccg tggaatatgc ccggactaaa
     6181 agccgtcggt tcttatcgct cggtactgga attgctcaat attccccatg cgaaattaat
     6241 catggactgc gtgatattac tttccgtaac cagttgcctg aactcggcgc tgtataccgc
     6301 gtcaaggatg ctctactcct taagtcgtcg cggtgatgct cccgcggtaa tgggtaaaat
     6361 caaccgcagt aaaaccccgt acgtggcggt gttactctcc accggcgcgg cattcttaac
     6421 ggtggtggtg aactattacg cgcctgcgaa ggtatttaaa tttctgatcg acagctccgg
     6481 cgctatcgcc ctgctggttt atttagtcat cgccgtttca cagttgcgga tgcgcaaaat
     6541 tctgcgagca gaaggaagcg aaattcgctt gcgaatgtgg ctttatccgt ggctcacctg
     6601 gctagtcatc ggctttatta cctttgtgtt ggtagtgatg ctattccgtc cggcgcaaca
     6661 gttagaagtg atctccaccg gcttattagc gatagggatt atctgtaccg tgccaattat
     6721 ggctcgctgg aaaaagctgg tattgtggca aaaaacaccc gttcataata cgcgctgatc
     6781 atgatcagga gtcacaccat gaccattacg tctctggatg gctatcgctg gctgaagaac
     6841 gatattattc gcggtaattt tcaaccggat gaaaaattac gaatgagttt gctgacatcg
     6901 cgttatgcac ttggcgttgg gccgttacgg gaagctcttt cgcaactggt ggcggaacgg
     6961 ctggtcacgg tggtgaatca aaaagggtat cgggtggcgt ctatgtcaga gcaggagctg
     7021 ctcgatattt tcgacgcccg cgccaatatg gaagcgatgc tggtcagtct ggcgattgcc
     7081 cgcggtggcg atgaatggga ggcagacgtt ctcgcaaaag cgcatctgct gagtaagctg
     7141 gaggcctgtg acgccagcga gaaaatgctt gatgagtggg atctgcgtca tcaggcgttt
     7201 catacagcaa ttgtggcggg ctgtggttct cactatttgc tgcaaatgcg tgaacggttg
     7261 tttgatctgg cggcgcgcta tcgatttatc tggctgcggc gaacggtgct ttcggtagaa
     7321 atgctggagg ataaacacga tcagcaccag accctgactg cggcggtact ggcgcgagat
     7381 accgcgcgcg ccagtgagtt aatgcgccag catttactga cgccaattcc cattatccag
     7441 caggcgatgg ctggcaatta actactcttc cggaatacgc aacacttgcc ccggataaat
     7501 tttatccggg ctttttagca tcggtttatt cgcttcgaag attttattgt acagattagc
     7561 gttaccgtag acctgtttgg aaatggcact cagagtgtcg ccagacttaa cggtataaaa
     7621 ctggctggca gtggctggtg tcgccgtttt cacctgatca tcaacactgg caataccgga
     7681 aatattcccc accgcaacaa ggattttctc cttcgcctcc tgactcaggc cgtcaccagt
     7741 gaccgtcgct ttgccgtcgg caatttgaat gttcacttta tcggcatcag gtataccggt
     7801 tttgctcaga tgctcctgca ccttcttcgc ctgatcgtct ttatcgtgct gacctgtaac
     7861 cgcgtcccag agtttttctc cggcatcttt cacaaaattg aacagaccca tagctacctc
     7921 attcgttaac ggaaacactc agaaagtgta gcagaggaac gcggcgcgtt aacgccgcgg
     7981 gggtgagcac aggtttaatc gcgggtttgc acccagaacg cgtgaatcaa accggggata
     8041 tacccaagca gtgtcagcag aatattaata atgaacgccc aaccgaaccc tttaccgagc
     8101 agcacgccga gcggcggcag aatgatggtg atgacgattc tccagaaacc catatgtact
     8161 c
//
LOCUS       DAPHBJ010000193         8161 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.195, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000193 DAPHBJ010000000
VERSION     DAPHBJ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.195"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..460)
                     /locus_tag="RHZ76_003792"
     CDS             complement(<1..460)
                     /locus_tag="RHZ76_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HDV2814352.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMS"
     gene            complement(484..1098)
                     /locus_tag="RHZ76_003793"
                     /pseudo
     CDS             complement(484..1098)
                     /locus_tag="RHZ76_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1097..1327)
                     /locus_tag="RHZ76_003794"
                     /pseudo
     CDS             complement(1097..1327)
                     /locus_tag="RHZ76_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            1518..3659
                     /locus_tag="RHZ76_003795"
     CDS             1518..3659
                     /locus_tag="RHZ76_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDV2814353.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQKLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILGTILQENGVTEWSPLFSEPHPYREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPCWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     SGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQESSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCCKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3735..7979
                     /locus_tag="RHZ76_003796"
     CDS             3735..7979
                     /locus_tag="RHZ76_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDV2814354.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPLLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDNGGRSIHFEPLFPGEISYSRSESLWLARGGVAEQHSSQPLSALWQ
                     VLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLTFHRAAEGDVAGAVTGVTDGAGRCFHLVLTTQAQRAEAFRKQRATSLSSPAGPRSA
                     SSSSAFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTASGELRAV
                     YDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLDYRFEYG
                     ERRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRR
                     TEYSLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKGRLTAET
                     SRSGETMRYSYDDPASELPTGIEDATGSTKQMAWSRYGQLLAFTDCSGYTTRYEYDRY
                     GQQIAVHREEGISTYSSYNPRGQLVSQKDAQGRETRYEYSAAGDLTAIVAPDGSRSEI
                     QYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGR
                     TQRYHYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGDPAEQWQYDEHGWLTTISH
                     TSDGHRVSVHYGYDDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLATRQEPDGL
                     PPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETVRSFGSMAGSNAAYELTSTYTLT
                     GQLQSRHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDID
                     IPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRHDEYGRLAEKTDLIPEGV
                     IRMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTG
                     WMSLSRKPEETWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAKARHR
                     SLAEVLQEDTGVTLPAELAELAVMLGRLERELRAGAVSAESEAWLAQCGLTVEQMESQ
                     MEAEYIPERRLHLYHCDHRGLPQALISPEGETAWCGEYDEWGNQLNEENPHHLYQPYR
                     LPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGINLYTYPLAPIRYTDPLGLE
                     RVISVYGPPAPDRAGAETPLVLTDMTGGVTIYYDPETGDSMTFDSSNRIDRRSQRGAG
                     DPYTGEVVGCETNESGISAAYGTTKIYTTDTRARWLHGGGSSLRDPYAPRQGWKPTMG
                     CTRAQNEDVDELCKKVTSWMYSHPGERIRYERFKTR"
BASE COUNT         1887 a         2083 c         2561 g         1630 t
ORIGIN      
        1 tactcattgc attcgtaccg gctaacgtat gctcccagga aatatcttca taaataaagg
       61 caattctctc ctgtggttcc agttcttttt ccaggacagc atgcggtgtg acaaaatcaa
      121 tacttgatat tcgggctttt gacagattta ttttataata aggctggtaa atacccgctt
      181 tactggttcg gtagagtata aattcacata ttgccatttt ttcattgtca ctgagtgctt
      241 ttgccagcaa tggtgaggat ttatcaaccg gtttggtgat aattaattca tgatgattga
      301 cattttgact tcttgagatg gaatgcatta acgcataaac catgatctga tctttatgtt
      361 ctggctgcgc tttgtttccg acagaatcaa gcgttgagca acctgctgaa attagccctt
      421 gtgtctctcc actcaaagat aaataactta tatttgccat gataatctcc tttaattaaa
      481 taattatttt tcggccgtgg taccggttgc cgcctctggc atgacgttat tcttcagcag
      541 cagccagcgg cttaacacgc cttcgagtcg ctcattaatt tgtttttcca gcgcatctgt
      601 attctgcttc tgcgcacggg catcatggta ctgcgtcaac agagactcaa cgggtgtttc
      661 ctgccccagt ccccgctccg cctgccagat gacatttttg atataggaaa gggttagtcc
      721 ctctttctcg cgctgcatca ctagatctgc aaaagcatgt aagtcctggc gggtctgcaa
      781 ccagccacgc aactgcgggc tactctgtgc gcgggttttg agtgcctcat tccattgtgt
      841 cgaggcctgt tgctgttgca ggctttccgg ccagcgactg tcggcaacac gcatcatctg
      901 catcccggtt tccagcggct gtaccgggga ggtatcaaga agttgttgca gcctgcgctc
      961 atagttttcg agttcaggtg aggccatcca caccgttgct gcgccctgag cggtgtcgtt
     1021 aacctgtaac agttgctgct gcaccggata gacctgccac caccacaatg ctgaggcgac
     1081 agccactgaa caggcgatct gccagggctt cctcccgttc tttggcagag agtttttcag
     1141 aaaagacgcc tggctgtgtg ttgtcacgca ggacggtcag cgcccgctgc aaaggctggt
     1201 cgccgctgat aacctgctcc agcgccccgg tcagttgtcc gatggactca aaattgcgga
     1261 tattcagatt gtccatcccg gcccgccagg tggcggtgta gtcgctgata tactgctcgg
     1321 tcatccggct atcaggccgg atgctgtttt tgtattacgc cgtagaaaga aaggttgtca
     1381 gtaaggggtt acgttcacgg gagatggcgg ctgccggaaa cttcggcggt agcgtgcctg
     1441 aacgcaagcg taacactgta ctgtcacagc cgctttaaca gcgaactgtc tgtttttcct
     1501 taaccccgga ggttgctatg tcaaccggat tacgtttcac gctggaagtg gacggcctgc
     1561 caccggatgc ttttgcggtg gtctcctttc atctgaacca gtcactctct tcgctttttt
     1621 ccctcgatct ctctctggtc agccagcagt ttctctccct tgaattccag cagatcctcg
     1681 acaaaatggc ctacctgacg atatggcagg gcgatgacgt acagcgccgg gtgaaaggtg
     1741 tggtgacctg gtttgaactg ggggagaacg acaaaaacca gaagctgtac agcatgaagg
     1801 tatgcccgcc gctgtggcgc acagggctgc gccagaactt ccgtatcttc cagaatgagg
     1861 acatcgaaag catcctcggc acgatattgc aggaaaacgg ggtgaccgag tggagcccgc
     1921 tgttcagcga gccacatcct taccgtgagt tttgtgtcca gtacggtgag actgattacg
     1981 atttcctgtg ccggatggcg gcggaggaag gcatcttctt ttatgaggag cacgcgcaaa
     2041 aaagtaccga ccagagcctg gtcctgtgcg ataccgtgcg ttatctgccg gagtcctttg
     2101 agatcccctg gaatccgaac acccgtaccg aggtaagcac cctctgcatc agccagtttc
     2161 gctacagcgc acaaatccgc ccttcttccg tggtgaccaa agactacacc tttaaacgac
     2221 cctgctgggc agggcgtttt gatcaggaag gccagtacca ggattaccag cgcacacagt
     2281 atgaagtgta tgactacccc ggacgtttca agggtgctca cgggcagaac tttgcccgct
     2341 ggcagatgga tggctggcgc aacaacgcag aagtggcgcg cggaacaagc cgttctccgg
     2401 agatatggcc gggacggcga attgtgctga cggggcatcc gcaggcgaac ctgaaccggg
     2461 aatggcaggt ggtggcaagt gaactgcacg gcgagcagcc gcaggcggtg ccgggacgca
     2521 gtggttcagg aaccacgctg aataaccact ttgcggtaat accggcagac cggacatggc
     2581 ggccacagcc gttgctgaaa ccgctggtgg atggcccgca gagcgccgtc gtgacgggac
     2641 cggcaggcga ggaaatcttc tgtgatgaac atggccgcgt gcgggtgaaa tttaactggg
     2701 accgctataa cccgtcaaac caggaaagtt catgctggat ccgtgtggca caggcgtggg
     2761 caggcaccgg ctttggtaac ctggcgatac cgcgtgtggg tcaggaggtc attgtggact
     2821 tcctcaacgg cgatccggac cagccgatca ttatggggcg tacctaccac caggaaaacc
     2881 gcacccccgg cagcctgccg gggacgaaga cgcagatgac gatccgctcg aaaacctata
     2941 agggcagcgg gtttaatgaa ctgaagtttg acgatgcgac agggaaagaa caggtctaca
     3001 tccacgcgca gaagaacatg aacaccgagg tgctgaataa ccgcaccact gatgtgataa
     3061 acaaccatgc cgaaaaaata ggtaacaacc aggcgatcac cgttaccaat aaccagatcc
     3121 agaacattgg cgttaatcag atacagacgg ttggtgtcaa ccaggtggaa acggtgggca
     3181 gtaaccagat tatcaaagtg ggatcaaacc aggttgaaaa ggtggggatc attcgtgcgc
     3241 tgacggtggg tgtggcttac cagacgacgg taggcggcat tatgaatacc tcggtggcgt
     3301 tgttgcagtc ctcacaggta gggctgcata aatcactgat ggtgggaatg ggctacagcg
     3361 tcaatgtggg gaataacgtc accttctcgg tgggcaagac aatgaaggaa aacaccggac
     3421 aaacagcagt ttattctgcc ggtgagcatc ttgaactctg ctgttgtaag gcaaggctgg
     3481 tgctgacgaa ggacggaagc atatttctca atggtacgca cattcatctg gaaggggagt
     3541 cggatgtgaa cggtgatgcg ccagtgatta actggaactg tggtgccaca caacctgtac
     3601 cggatgcgcc tgtgccgaaa gatttacccc ccggaatgcc ggatatgcgg caattttgag
     3661 tcaggtgcaa aaaattaaaa cggaactggt gcagtgacca gataaaccat attaaaagca
     3721 gaaggaattg attcatgggc ggaaaaccgg cggcgcggca gggtgatatg acccgcaagg
     3781 gactggatat tgtgcagggt tcagcggggg tgctgatagg tgcgccgacg ggagtggcct
     3841 gttcggtatg tccgggaggg attacctatg ctaacccggt gaacccgctg ctgggtgcga
     3901 aggtgctgcc gggcgagacg gaccttgcgc tgcccggccc gctgccgttt attctttccc
     3961 gcgcctacag cagctaccgg accagaacac ccgcgccggt gggggtgttt ggtcccggct
     4021 ggaaagcgcc cttcgatatc cgcttacaga tacgcgatga aggcctgata ctcaacgaca
     4081 acggcggcag gagcattcac tttgagccgc tgtttcccgg cgagataagc tacagccgca
     4141 gcgagtcact ctggctggcg cggggcgggg tggcggagca gcacagttcg cagccgctaa
     4201 gcgcactctg gcaggtgctg ccggaagatg ttcgcctgag tccgcatgtg tacctggcga
     4261 caaacagcct tcaggggccg tggtggatac tcagctggcc ggagcgggtg ccgggggcgg
     4321 acgaggtgct gccgccggag ccgcccgcat accgggtgct gacgggcgtg gtggacggct
     4381 tcgggcggac gctgactttc caccgggcgg cggagggtga cgtggcgggc gcggtgacgg
     4441 gggtgacgga cggcgcgggc cgctgttttc acctggtgct gaccacacag gcgcagcggg
     4501 cggaagcctt ccgtaaacag cgcgccacgt ctttatcttc ccctgccggt ccccgttctg
     4561 cttcctcttc ttctgctttc cccgacacgc tgcccgccgg tacagaatac ggtgccgata
     4621 acggtatccg gctggaggcg gtgtggctga cacacgaccc ggcatacccg gatgaacagc
     4681 cgactgcgcc gctggcgcgc tacacgtaca cggccagcgg agaactgcgg gcggtgtatg
     4741 accgcagcgg gacgcaggtt cgcgggttta cttatgatgc ggagcacgcc gggcggatgg
     4801 tggcacacca ttatgcgggg aggccggaga gccgctaccg gtatgatgat accggtcggg
     4861 tgacggagca ggtcaacccg gaggggctgg actaccgctt tgagtacgga gagcgccggg
     4921 taatcatcac ggacagcctg aaccggcggg aggtgctgta cacggagggc gaaggcggcc
     4981 tgaagcgggt ggtgaagaag gagcatgcgg acgggagcat cacccgcagc gagtatgatg
     5041 aggcggggag gctgaaggcg cagacggatg cggcggggcg gcggacggag tacagcctgc
     5101 atatggcgtc gggcgcggtg acggcggtga cggggccgga cggcaggacg gtgcggtatg
     5161 gctataacag ccagcggcag gtgacgtcag tgacgtaccc ggacgggctt cgcagcagcc
     5221 gggagtatga tgagaaggga aggctgacgg cggagacctc gcgcagcggt gagacgatgc
     5281 ggtacagcta tgatgacccg gcgagtgagc tgccgacagg gatagaggat gcgacgggca
     5341 gtacaaaaca gatggcatgg agccgttacg gtcagctgct ggccttcacg gactgctcgg
     5401 ggtacacgac gcggtatgag tatgaccggt acggtcaaca aatcgccgtt caccgggaag
     5461 aaggcatcag cacttacagc agttataacc cgcgtgggca actggtcagt cagaaggatg
     5521 cgcaggggcg ggagacccgt tatgagtaca gtgccgcagg cgacctcacc gctatcgttg
     5581 ccccggacgg cagccgcagt gagatacagt atgatgcgtg gggaaaggcc gtcagcacca
     5641 cgcagggcgg tcttacgcgc agcatggggt atgacgctgc cgggcgcatc accgtgctga
     5701 ccaacgagaa cggcagccag tccacgttcc ggtatgaccc ggtcgacagg ctgacggagc
     5761 agcgtggctt tgatggcagg acgcaacgtt accattatga cttgaccgga aaactcacgc
     5821 agagtgaaga tgaggggctt atcaccctct ggcattacga tgcgtcagac cgtatcaccc
     5881 ggcgcacggt gaacggcgac ccggcggaac agtggcagta tgatgaacac gggtggctaa
     5941 ccaccatcag ccataccagt gacggccacc gagtgtcggt ccactacggc tatgacgata
     6001 aaggccgcct gacgggcgaa cggcagacgg tggagaaccc ggagacgggg gagatgctgt
     6061 gggagcatga gacggggcat gcgtacagcg aacaggggct ggcgacccgt caggagccgg
     6121 acggtctgcc gccggtggag tggctgacgt atggcagcgg ttatcttgcg gggatgaagc
     6181 tgggcggaac accgctggtc gagtacacgc gggaccggct gcaccgtgag acggtgcgca
     6241 gcttcggcag catggcaggc agtaatgcag catatgaact gaccagcaca tacaccctca
     6301 cagggcagct acagagcagg cacctgaacc tcccgcagct tgaccgggac tacgactgga
     6361 acgacaacgg acagctaatc cgcatcagcg gcccgcagga gagccgggag taccgttaca
     6421 gtgacacggg aaggctgacg ggcgtccaca ccacggcggc gaacctggat atcgatatcc
     6481 cgtatgcgac ggacccggca ggaaaccggc tgccggaccc ggaactgcac ccggacagca
     6541 cgctcacggc gtggccggat aaccgcatcg cggaagatgc gcactatgtc taccgccacg
     6601 atgaatacgg caggctggcg gagaagacgg acctcatccc ggaaggggtt atccggatgc
     6661 acgacgagcg gacccaccac taccattacg acagccagca ccgtctggtg ttctacacgc
     6721 ggatacagca cggcgaacca caggtggaga gccggtacct ctatgacccg ctgggccgcc
     6781 ggacgggaaa acgggtatgg cggcgggagc gtgacctgac ggggtggatg tcgctgtcgc
     6841 gtaaaccgga ggagacctgg tacgggtggg acggtgacag gctgaccact gtacagaccc
     6901 aacagacaag aatccagacg gtataccagc cgggaagctt cacgccgctc ctgagaatcg
     6961 aaacagagaa tggcgaacag gcgaaggcgc ggcaccggag cctggcggag gtgttgcagg
     7021 aggacacggg ggtgacgctt ccggcggagc tggcggagct ggcggtgatg ctggggaggc
     7081 tggagcggga actgcgtgcg ggagcggtga gtgcggagag tgaggcatgg cttgcgcagt
     7141 gcgggctgac ggtggagcag atggaaagcc agatggaggc ggaatacatc ccggagcgga
     7201 ggctgcatct ttaccactgt gaccaccggg gactgccgca ggcgctcatc agcccggaag
     7261 gggaaacggc gtggtgcggg gagtatgacg agtgggggaa ccagcttaat gaggagaacc
     7321 cgcatcacct gtaccagccg taccgtttgc cggggcagca gtatgatgag gagtcgggac
     7381 tgtattacaa ccgtcaccgg tactacgatc cgttgcaggg gcggtatatc acccaggatc
     7441 cgatagggtt gaagggggga ataaatctat acacgtatcc actggcacca atacgttaca
     7501 ctgatccact ggggttagaa cgagttataa gtgtatatgg gcctcccgct ccagaccggg
     7561 caggtgctga aactcctctt gttcttactg atatgactgg aggagtgact atttattatg
     7621 atcctgaaac aggtgattca atgacttttg atagtagcaa tagaattgat cgtcgttctc
     7681 aacgtggtgc tggtgatcca tatacaggtg aagtcgttgg ttgtgagaca aatgaatcag
     7741 gaatatcggc agcatatggc acaacgaaga tctatactac agatactaga gcaagatggc
     7801 tacatggtgg gggatcatcg ttgcgcgatc cttatgctcc tcgacaaggc tggaaaccaa
     7861 ctatgggatg tacccgtgca cagaatgaag atgtagatga gttgtgtaag aaagtgacaa
     7921 gttggatgta ttcgcatcct ggagagagaa ttagatatga acgttttaag acacgttaat
     7981 gcgattaaca tgggagtgtg cattctaatt ttggttattg gatgtgcaaa tgctaaagtt
     8041 cttaaacgat gtatattacc cgctcctgat ggtggcgtag aaaaactatc aaaacccaac
     8101 ttacatggag taattaaaaa agttgatttg aacacaaatg aagcggaaat taaaataaaa
     8161 g
//
LOCUS       DAPHBJ010000194         8106 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.196, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000194 DAPHBJ010000000
VERSION     DAPHBJ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.196"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(39..728)
                     /locus_tag="RHZ76_003797"
     CDS             complement(39..728)
                     /locus_tag="RHZ76_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDV2814355.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(822..2969)
                     /gene="fdhF"
                     /locus_tag="RHZ76_003798"
     CDS             complement(822..2969)
                     /gene="fdhF"
                     /locus_tag="RHZ76_003798"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(2550..2552),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDV2814356.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INGIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(3167..4633)
                     /gene="mdtP"
                     /locus_tag="RHZ76_003799"
     CDS             complement(3167..4633)
                     /gene="mdtP"
                     /locus_tag="RHZ76_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HDV2814357.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYSMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLATGVAQLYYSMQASYQMLDLLEQTHDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIVAVKGQITETRESLRALIGAGASDMPE
                     IRPVALPQVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLHTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAGPVVEKK"
     gene            complement(4630..6681)
                     /gene="mdtO"
                     /locus_tag="RHZ76_003800"
                     /pseudo
     CDS             complement(4630..6681)
                     /gene="mdtO"
                     /locus_tag="RHZ76_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
     gene            complement(6681..7712)
                     /gene="mdtN"
                     /locus_tag="RHZ76_003801"
     CDS             complement(6681..7712)
                     /gene="mdtN"
                     /locus_tag="RHZ76_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HDV2814358.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAKRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(7731..8006)
                     /locus_tag="RHZ76_003802"
     CDS             complement(7731..8006)
                     /locus_tag="RHZ76_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814359.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         1859 a         2200 c         2146 g         1901 t
ORIGIN      
        1 gcctgatgcg acgcttgccg cgtcttatca ggcctatctt aaccgttggt taatttttca
       61 aactcttcac acccggtatc aaacccttcc atacagctca ggttcaacca gtgcagcgcc
      121 ttttgcttgt tcttctcgat aaaaccttct tcaccgttta agaacatcat tcccgcccag
      181 tactcggaat aaccggttcg ggaaattgca gagctgcgtt tgaaatacca ggttgccttg
      241 tcatcgtcgg ccttaatgcc cacgccgttg gcgtaaatca aaccaagcag catttgggca
      301 tcgacggcag agtcgttctc cagatcttcc gaggcgtttt ctaacagcga aattgccttt
      361 ggataatccg gtttacccgc ttgagtattt accagaatat gcgccagcgt tacttcacct
      421 tctttactgc ccgcttgcgc cgctttctct gcaagaactt ttgcttgtgg atagtccaga
      481 ctgaccggat tggtgatttt gatctgcgcc agcagcgcgc aggcatcagc atcaccgctg
      541 tcggcggctt tctgtgccca atattcggct ttgctcaaat cgccggagct aaaccagctg
      601 tcggcaagaa aatattgtgc gcgtcggtcc ccggcctctg ctgcctttaa atactggctg
      661 ccaggctcgg agtcgttggc gtgggcaaag aatgtcaaaa acaacatcaa tgcgataatt
      721 tttttcattt tgataatacg attgggtagg gagtaaccag tataaagagt tcgaatggat
      781 aaaaaaacag cctccgaaag gaggctgtag aaaggacggt attacgccag tgccgcttcg
      841 cgcaggcgag ttttcaactt gttgtactcg tcaatcacgt actgctcggc ggcgcgctga
      901 tcggcgatcg gctcgacgcg cacggcgcag tatttgtatt ccggcgtttt cgtaatcggg
      961 cttaagtttt cggtaaccag ctcgttacag gcaccaatcc accactggta ggtcatgtaa
     1021 atcgcccctt tgttcggacg atcgctgacc tgcgcacggg tgataatttt gcctttacgc
     1081 gagtgcaccc aaaccaatgc ctcatcttca atacccagac gtttggcgtc ttcggtattg
     1141 atttgtgcgt agccaggttc atcagccagc gccgccagtg ccgcacagtt accggtcatc
     1201 gaacggcaag agtagtgacc aacttcacgc accgttgaca gtaccatcgg gtactcgtcg
     1261 gtgagtttgt cgattggcgc tacccagtcg caggtgaaga actgcgccag accgttcggg
     1321 gtatcaaact tctctttaaa cagataagaa gtcccctgat cggcatctga agtatcgcgg
     1381 caaggccact gaatgaagcc cagttcgccc attttctcgt aagtcgcacc gtagaaatcc
     1441 gggcacagat gacgcaactc atcccagatc tcctgggtgt tgttgtagtg catcggataa
     1501 cccatacggg tggcgatttc actgatgatt tgccagtccg ttttcagatc ccatttcggt
     1561 tcaaccgcct tgaagaaacg ctggaagcca cggtcagccg cagtaaacac gccttcatgc
     1621 tcgccccacg acgttgacgg taaaataaca tccgccgccg acgcggtttt ggtcataaag
     1681 atgtcctgaa cgataaccag ttccagatct tcaaaggctt tacgtactgc cgacagctcc
     1741 gcgtcagttt gcagcggatc ttcgcccata atgtacgcgg cacgcacttc gccatgcgct
     1801 gcgcggtgcg gtagctcgct gatgcgatag ccggtatgcg ctggcaggct ttccacgccc
     1861 caggctttgg cgaatttctc gcggttagcc ggatctttca cgtactggta tcccggatac
     1921 gtatccggca gcgcgcccat atcgcaggca ccctgtacgt tgttctgacc acgaaccggg
     1981 ttaacacccg catgtggctt accgaggtta ccggtcagca tcgcgaggct ggtcagagaa
     2041 cgcacggttt ccacgccctg gtagaactgg gttacaccca tgccccacag gatggcggcg
     2101 cttttcgcct gggcatacat ccgtgccgcc tgacgaatct cactggcgct gacgccggtg
     2161 atatcttcaa ccgactccgg cgtgtagcct tcaacgattt tacgatactc ttcaaagcct
     2221 tctgtacggg aagcgacgaa tgctttgtcg tacaaatttt cttcaataat gacatggccc
     2281 atcgcattca acagcgcgat gttcgagccg tttttcagtg caatgtgcat gtcagcaatg
     2341 cgcgcggttt caattttgcg cggatcgcag acgataattt tcgccccgtt acgtttagcg
     2401 ttaattacgt gattcgctac gattgggtgg gaatccgccg ggttgtaccc gaaaacgaac
     2461 actaaatcgg tattatcaat tccgttaata gcattgctca ttgcgccatt accgaccgat
     2521 tggtgcagac ctgcaaccga tgggccgtgt cagacacgag cgcagcagtc aacgttattg
     2581 gtaccaataa cggcgcgcgc aaatttttgc attacatagt tggtttcgtt acccgtacca
     2641 cgcgaggagc cggtcgtctg gatggcatcc ggaccgtact tctctttgat ggcgctcagg
     2701 cgctcggcaa cgtaattcag tgcctcatcc caggaaacag gttcgagttt gccgccacgc
     2761 tggcgacgga tcatgggggt tttcaggcgc ggggtcagga tctgggtatc gttaatgaag
     2821 tcccagccat aataaccctt cagacacagg gtaccctggt tggttttccc ctgcgctgcc
     2881 tccgcccgga cgattttgcc gttatcgacg accaggttga ttttgcaacc tgatgcgcaa
     2941 taggggcaaa ccgtgacgac ttttttcatc ggtctcgctc cagttaatca aatcacgcat
     3001 acgcgctctc gactacagta tgcatctttt atgccacatt ttatgtgggg tcattccctg
     3061 atattacggg cactatttat tcaaaactct gacgaaaaac aggctgtcgt cagttttgac
     3121 gtgacgaaac gaaataccgc gtgacagcca tcacgcggca gacattttat tttttctcga
     3181 cgacgggacc tgcctgatac ccgccgccca gcgatttcat caactgaatg ctttggatca
     3241 cccggcggct gtccagcatc agtaatgaca tctcttctgc aagcactggc aaccgggctt
     3301 cggtggcctg taagcggctg gttaagccgc gctgataggc ggcctcggca gcgcgctggg
     3361 taaagcgcgt ggcttccacg cgttcagcct gcatttctcg ctcgtcgttg agcgtttgca
     3421 gacgcgtgcc gttgacggca acgtcacgca ccgcgttcag tactgactgg ttgtaacgtt
     3481 caatcatcat gttgctggcg gcgcgcgtgc cttcgagatt ggcattcaac cgtccaccgt
     3541 caaacagcgg caatttcaga cccgggatga agttgaactg gcgactggtt tttttgaata
     3601 aggtatgcag atggatggag tccagaccga aaaacgcttt gatatcaaag ctcggataga
     3661 acaacgcccg cgcggaatcc acctgatcta atgacgcctg aacataccag cgcatggctt
     3721 gcagatccgg gcgtctggcg agcaactcat aagagagtgt cgccggaatg ccggtctgga
     3781 cttgcggtaa tgccaccggt ctgatctccg gcatatcgct ggctcccgcg ccaatcaatg
     3841 cacgcagaga ttctcgcgtt tcggtgattt gccctttgac ggcaacaatt tgtttatcga
     3901 ccgccagaat ctgtgcccgc gcgccgtgga aaggcacttg cgcttccaga ccgtgcgcca
     3961 ctttactctg gtgcgctttc accgcgtaat caatcacatc gtgagtttgt tctaacagat
     4021 cgagcatctg atagctggcc tgcatactgt aataaagctg cgctacgccc gtggccagcg
     4081 atagctctac tgctgcggtt tctgccagcg cggcattatg cgcgccaatg gcggcggcaa
     4141 ccgctgagcg atgcacaccc cacaaatcaa gatccagtcc ggcaaacaaa cctactgtgg
     4201 cttccgtata gtacggcccg tccataccca gtgctggcgc atccattgaa taagggctta
     4261 aaaagccgtt cgccgagaca cgttggcggt tgagcatccc taacgctgcg acctgtaatt
     4321 gtgaaccggc atctaacaaa tcggcctggg actgcgcttt ttcttcccgc agtttcgctt
     4381 cggcgagggt gtgtgaacca cttagcgtcc gttggatcag cgcatccagc tgcgggtcat
     4441 tgagttgttt ccaccactgc gcctgcggcc agccggagct ggcaagatga atatcgtcgg
     4501 ccagtttgat ttgttccggt ttgagctgtt gatgaggtgc agaatcctta cgtaccaggg
     4561 cacagccgga aatcagcgtc gtgctgccga gaatgctgca caacagcaga cgtgaaagtt
     4621 gacgattgat cattgcgtgg ctccttgtgc ctgttccgtg gcgggcgtta atgccggggc
     4681 tgtccagtct ggcaagcggg cgaaaagctg gcagacgtgt tgctcctgtt ttaataaggt
     4741 gggcaggatg gcctgagagg cgggtgtctc ttcaagcgtt atttgaggag aacggctgag
     4801 tgcggttgcc agaccggcag cgtatttctc cagggcgtcg gcaaactgac ctgctctgtc
     4861 cggctgtagt gcgttatcca gcgcctgcgc cgagttccag gtggcatccc aggcgtgaag
     4921 aagatcgcgg ccctgacgaa taaccgtttg cgaacgttca atcagtaatg cgcgttcttc
     4981 gctgtccagt tgacgctcca gcgccacgcg ttggcacatc tcttcacagg cattaaacgc
     5041 cgcatgcaga cctatacgaa tttgcagata agtgcgcaga gccgtgactt cctgctggcg
     5101 tgggatccgc attactttac ttaacatacc cagcgcgcca gccagttttt gcggcagcgt
     5161 gcgcgcttca ctctcaggcc agacaaaggt gtaaatcacc gcggacacca cggtaccaat
     5221 gatgataccc agggcgcgat cgcgaatttc caccaggtcg tacaccgggc caaaaacgtt
     5281 ttcgagcgtg gcgagcgcga aggtgaccac catctgtgtg ccgatataag aagaacgttc
     5341 agagctggtg gcaatccatg cgcccaacag gaaaataggt gccagcacaa acagcaattc
     5401 gacaatattg tccagccagg gcatgaccag tagcgtgaat aacagcgcca gaatcgcgcc
     5461 gcaaaaagcc ccgccaaaac gcagcaccat cttctggtac gacgaaccga cattcgggtt
     5521 agcgacgatc acgcaggtca gcatacaggt gtgaatgcct tcccagtcga cgccgctgta
     5581 gaaggtgtaa cagatcaaac aggcgagcag cgtttttacc gcgtagcgca tatagtctgg
     5641 attggtaaaa gcatcggcgg ccattgatgg tggtttggcg gcgggcgttg gcggcgtatt
     5701 cgggttcatc tgacccagtt gtaacaacgt ctggcagata ttctccaggt tacattcccg
     5761 tgccgccatc gcttcacttt cactgatccg ccagtcgctt tgccagcact gaccttcggc
     5821 aatggaatgc tgcagcttgt tgatttatga ggctaatttt tgtcggaatt caataattgc
     5881 ctgagaatca gcaaaagagg tgggatcgta gcgattcagc gtcgagtaaa tgtaggttac
     5941 cgttgccacg cagctttgcc accatgcgtt ttgagttcgc cagttggcat cgtccgcgag
     6001 gcaaaagaca ttgagttttt gtagcgccag cgcctctctt tcaatccgcg tttcgggtag
     6061 cggtgcgagg ctgtctgtca ggtgactaat ggcatcatca agccgatcat taagcgcctg
     6121 atgcatttgc gaaatggcac gactgggaaa ccacagcacg ccgattaacg tcatcagcaa
     6181 ggttggatag aggccaacaa cgatacacca cagcgttaag cgcacgacca cttccggata
     6241 gtcgagcatg gcggggaagg tttgcccgta aatagcgaca atggcgacgg cgaaaaagac
     6301 cagccccaag cgatgggtgc gcatcaaaaa catgcagccc atcaggatcg gtccggcgat
     6361 gatcaatcgg atcaacggtt cgccgtatga ccatttgtag atcaaaaaca ggctgccgat
     6421 ctccagcacc gtggcaacca caaacaagat cgcgacaaat ttggtgtaaa acgcgttcga
     6481 ctgaataccg taaaacagca ctgccagcga taacgccaca aaagggatct caaaggtcat
     6541 cgagatcaga atcaccagca ggcagcctac ccagagttgc acggtctgcg gcacgcgacc
     6601 tggtcgccgc tcgcttaact cttcatgaaa gaacgccagc agcctgacca ccggtaatgg
     6661 cagggagttg agcgcgctca ttattgcggc tcaagattag cgactgccga agcgccgatg
     6721 cggaacattt ccgggtcagg tttatcgacc atgattttga ccggaaaacg ctgggcaacg
     6781 cggacccagt taatagaacg agacactttc ggcaggccgc ccagcaccag gccgccgtca
     6841 tccggtagca cgccgtagcc aatcgaatcc actttaccct cgaaggtttt gccgctgtca
     6901 ctcatcaggc gaatcgttgc gggtgtacct gagcgaatat ttttcagatc ggtttcgcgg
     6961 aagttggcga tcacatacca gtgacgagtg tcgattaggg taaaaatagg gcgcatggca
     7021 gaagcaaatt gcccgacgga ggttttgagg gaaatgaccc ggccatcaaa cggcgcgcga
     7081 acggtcgcca tttccagatg cagtttggtc agggcaatat ccgcttcgac cgccgcacgc
     7141 ttggcaacta atgcatccac gccgctgacg gcgctggcgg ctgactgcgc ctgtaacaat
     7201 acggcattaa gatccgcttc tgcggcgcgc tgcgccgttc ttgcacggtc aacatcttcc
     7261 gctgagacaa aaccttcttt cagtaatggc tcggtgcggc gtaatgtatc tgtggcctgt
     7321 ttcgcggcgg cacgggcttt ttctaccgtg gcattaaccg agtcggcacc aaactgttgc
     7381 gcgtcaacgc tacgctgggt gagcataatt tgcttatcca gcgccgcgag ggaggcttca
     7441 gctttcgcca gattggcttc gtacgggcgc gggtcgatgc ggaacagcaa atcgccctgt
     7501 ttgactgcct ggttgtcggt gaccgccagt tctacaatgc ggccgctgac ttccggtacc
     7561 acatcaatgg tatctgctga cgcgtaagcg tcattagttg atggcgcact gtctacgcgc
     7621 cagataacga aaacaagggc aaccagcgcc aacgcaacca ctaacagagc agggaattta
     7681 ctgcgaggag cttttttcgg cgtactttcc atgagcgcgt ctcaaataga ttagaaaaat
     7741 gccagccaga acagcatggc gtagagagca aaaagggcgg tataaataat tccgacaaat
     7801 gccagattga tatttgcccg ctgaataact cgcctcgtga tgagcgtcaa aataaggctg
     7861 gcaatggcgc agaaaaacca gctgggataa taagcgccga tcaccgggat tgccggagat
     7921 aatgagcatc ccgaaaccat gaagacggga attatccagg ctgttttttt gagttgcatc
     7981 gccatgcgag agagaactgt tggcatatta aatacatcct gtctgctgcg aacaatatct
     8041 gtagtgaata tcaatatcca ttgaaggcgt cagtatggct attttcttga tgaataaaaa
     8101 tagctt
//
LOCUS       DAPHBJ010000195         8093 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.197, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000195 DAPHBJ010000000
VERSION     DAPHBJ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.197"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..1091
                     /locus_tag="RHZ76_003803"
                     /pseudo
     CDS             1..1091
                     /locus_tag="RHZ76_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(1060..2796)
                     /gene="aceK"
                     /locus_tag="RHZ76_003804"
     CDS             complement(1060..2796)
                     /gene="aceK"
                     /locus_tag="RHZ76_003804"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HDV2814360.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3077..4381)
                     /gene="aceA"
                     /locus_tag="RHZ76_003805"
     CDS             complement(3077..4381)
                     /gene="aceA"
                     /locus_tag="RHZ76_003805"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HDV2814361.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4411..6012)
                     /gene="aceB"
                     /locus_tag="RHZ76_003806"
     CDS             complement(4411..6012)
                     /gene="aceB"
                     /locus_tag="RHZ76_003806"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HDV2814362.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6281..7210)
                     /gene="metA"
                     /locus_tag="RHZ76_003807"
     CDS             complement(6281..7210)
                     /gene="metA"
                     /locus_tag="RHZ76_003807"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDV2814363.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQDQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEFFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7367..7810
                     /locus_tag="RHZ76_003808"
     CDS             7367..7810
                     /locus_tag="RHZ76_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDV2814364.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRTELEDLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRVLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2011 a         2036 c         2038 g         2008 t
ORIGIN      
        1 tatattaatg atatttatca gaatgttgat tacaaacccc atcaagatga ttattcttca
       61 gctgaaaaat ttctcactca cttcaacaaa aaatgcaaaa accagacttt ggcgttgatt
      121 tccagccgtc ctgaggggcg ttgcgttgct ggctgcggtg atttcgggct agttatgaaa
      181 gcatattttg acaagatgga atcaaatggc atcagtgtta tggcagccat attactggtg
      241 gataaccatg ctttgacggt ccggctaaga ataaagaaca caactgaagg atgtacccat
      301 tacgtggttt cggtttatga tcctaatgta actaacgata aaataagaat catgagcgaa
      361 agcaaagagg atattaaaca ctattctctg atggatttta tgaatgtaga ttatagcctc
      421 ctgaaatggt caaatgatca tgttattaac caatctgttg caataattcc agcacttccg
      481 aaagaacagc tattgatgtt aaaaggatct gtggatgaaa taacccctcc attatcacca
      541 gcaacgatga atttgctaat ggcaattggt cagaatcacc aacttacgca actgatgatt
      601 cagctccaga aaatgccaga actacataga acagaaatgt tgactgccta taatagtgga
      661 catatgaacg ttattaatac tatttttaac gcattaccca ctctgtttaa tacgtttaaa
      721 ttcgataaaa aaatatgaaa cccctcctcc tggcaaataa ttctaatgaa tatcccggtt
      781 tgttttcagc gatacagcat aaacaacaaa atgttgtaga gacggtttat cttgctttat
      841 ctgaccatgc acgcctgttt ggatttaccg ctgaagatat tatggatttt tggcaacaca
      901 aagcgccaca aaaatactct gcctttgagt tggcttttga attgggtcac cgggttattg
      961 ctgaattaat ccttaataca ttaaataaga tggctgaaag ctttggcttt acggataacc
     1021 ctcgatacat tgcggagaaa aattatatgg aagctttact caaaaaagca tctccccata
     1081 ccgtacgcta aatctttgtc tgcgccgata cgcataaaca tcttccacat gcccttcacg
     1141 tatgcggttt tgtagtgcgc gccagtaatc agcgcggaac aggtcggcgt gcatctcttc
     1201 aaacagcgga ccaatacgcg ggtcggcgca tagccagtgg cgaaactctt ccgggaaaac
     1261 atcgcccggc gagacgctgt accacggttc gctggcaagt tcgtcttccg gatagcgcgg
     1321 cggcgggatg tcgcggaaat tcacttccgt catgtagcaa atttcatcgt aatcataaaa
     1381 aaccacacgc ccgtgacggg tgacaccgaa gtttttaaac agcatgtcgc cagggaaaat
     1441 gttagcggcg gcaagctggc gaatagcgtt accgtattct tcaatggcgt cgcgcaactg
     1501 ctgaccttcc acttgttcca gccagatatt gagcggcacc atccgccgct caatataaag
     1561 atggcgaatc acaatttgtt cgccaagatc ggtgattttt tccgctgctt cctggagcag
     1621 taattccatt aatgccgggg aaatatgccg cttctccagc acaaagtttt caaactcctg
     1681 ggtgtccgcc attcggccca cgcgatcgtg ctctttcacc agttgatagc aggcacgaac
     1741 gtgagcggca gacatctctt tctgcggcgc gaacttgtct ttgatgactt tgaatacccg
     1801 atcaaagccc ggcagcgtaa acaccaacat caccattcca cgaatacccg gcgcttcaat
     1861 gaactgctca ttacagccct gtagataaac gagatattcg cggtagcttt cggttttggc
     1921 gtgcttctgg cagccgatag ccatatacaa ttcagcagtg gttttacctg gcagaatttc
     1981 ccgtagccac tcgaccagcg ctgcgggcag cggtgcataa accataaaat aagaacgcgc
     2041 aaagccaaaa acaatgctcg cttcggcggt cgtcgtcagg caggtatcaa taaataactc
     2101 gccgtcgtcc gtctggtgga tcggcagtaa gaagggcaac gtgccggaag gcgtgatcag
     2161 tttgcccacc agccaggcgg ctttattgcg gtaaaacagt tcgttcgcca cctgtaaatg
     2221 actttccgcg aggttgtctg tccccagcgt ttccgtcaga tggcgaatga tgtaatgaat
     2281 gtcacggctt ttattctgcc agcgcaggcg cagcggcagg tcgctgataa cgcgcatcag
     2341 cagagattcc cagccgtgat cggggtgaaa gtctttcgcc agcgggcggg gaatggtacg
     2401 aaagcggcgc tctggctggg agctaaagat aaaaagccgc tcgggagtaa gcgagcggtg
     2461 gtcaaataac cgacagtaca cggagttaaa aaagctctcc gcaatctcga agcgcgggta
     2521 atccggcaac agccgcgtgt aatgctcttt aacgcgcagt aaaaatgccg cgtccgtact
     2581 ttggccgtta gtaatgcagc gcagttgctc cacgaccaga ccaacgtgat gatcgtaaag
     2641 atggatacgg tttttcatcg cctgctggac agcatgccag tcggcctgtt cgaaacgctg
     2701 ctgcgccccg gaagtcactt cgaggaatcg accatactga gcgtcgaagc cttgcaaaat
     2761 ggtttgagca atcagtaatt ccaggccacg cggcatttag cgccctcatc aggagcagag
     2821 aattgccgga tgcggcgtaa atgccttatc cggcctacgt tcggcaacgg ttgtaggcat
     2881 gataagacgc gcaagcgtcg catcaggcat cgtgcaccga ttgccggatg cggcgtgaac
     2941 gccttatccg gcctacgttc ggcaacggct gtaggcatga taagacgcgc aagcgtcgca
     3001 tcaggcatcg tgcaccgatt gccggatgcg gcgtgaacgc cttatccggc ctacagtcag
     3061 caacggttgt tgttgcttag aactgcgatt cttcggtgga gccggtcagc gctgtaaccg
     3121 acgacgtgcc gccctgaata atagtcgtca ctttatcgaa gtaacctgta cccacttcct
     3181 gctggtgaga tacgaaggta tagccatctt tcgcggcggc aaattccggc tgctgcactt
     3241 tctcaacgta gtgcttcata ccctcgccct gggcataggc gtttgccagg tcaaacatgt
     3301 tgaaccacat gctgtggata cctgccaggg tgatgaactg gaacttgtag cccatatccg
     3361 acagctgctg ctggaagctg gcaatagttt tgtcgtcgag gtttttctgc cagttgaacg
     3421 acggcgagca gttataagcc agcagtttgc ccggatattt cgcgtggata gcttgtgcaa
     3481 agcgacgcgc cagttccaga tccggcgtgg aggtttcaca ccagaccagg tcagcatatg
     3541 gcgcatacgc caggccacgg ctgatcgctt gctcaatgcc cgcatgagta cggaagaagc
     3601 cttcactggt acgctcgcca gtaataaatt cactgtcata cgggtcgcaa tcggaggtaa
     3661 tcagatccgc cgcatcggca tcggtacgag caaccagcag ggttggaacg cccgtcacgt
     3721 cagctgcaag acgcgccgcg accagtttct gaatagcttc ctgagttggc accaaaactt
     3781 tgccgcccat gtgaccgcat ttcttcactg acgccagctg atcttcgaag tgaactgccg
     3841 ctgcaccggc ttcaatcatc gctttcatca gttcaaaggc attcaggaca ccgccaaaac
     3901 cggcttccgc atcggcaacg atcggcagga agtaatcgac atagcgcgga tcgcccggct
     3961 caatgcctgc ggaccattgg atctgatcgg cacgacggaa ggtgttgttg atccgctcca
     4021 ccacagctgg caccgagttt gccggataga gcgactgatc cggatacatg ctggccgcca
     4081 ggttagcgtc cgccgctacc tgccatcccg acagatagac tgcttcaata cccgctttcg
     4141 cctgttgcag cgcctgaccg ccagtcagtg caccgaggct gttgatgtag ccttttttcg
     4201 actcgccatg cagcagacgc cacattttcg ccgcgcccag ttgcgccagg gtgcattcag
     4261 gattgactga accgcgtaat ttcaccacat cttccgcgct gtatgggcga gtaatgcctt
     4321 cccaacgcgg ttgagtccac tctttctgta attcttcaat ttgttgtgta cgggttttca
     4381 tgtgcagatg ctccatagtt atgtggtggt ttacgctaac aggcggtagc ctggcagggt
     4441 caggaaatca attaactcat cggaagtggt gatctgttcc atcaagcgtg cggcatcgtc
     4501 aaaacgcccc tgggagaaac gttcttcgcc cagttcgctg gcaatgactt tcatctcttc
     4561 acccagcatc tggcggaaca aggctttggt caccggtttg ccattgctca acgttttttg
     4621 atgatggatc cactgccaga ttgaggtacg ggaaatttca gccgtcgccg catcttccat
     4681 caggccataa atcggcacgc agccgttgcc ggagatccat gcttcgatgt actgcactgc
     4741 cacgcgaatg ttggcgcgca taccttcttc ggtgcgttca ccatcacaag gtgccagcag
     4801 ctgatcggca gtaatcggcg cgtcttgttc gcgcatcact tcaagctgat ttttacggga
     4861 gccgagaatg tcgttgaata ccgccattgc cgtatccgca aggcctgggt gagcaatcca
     4921 tgtgccatcg tgaccgttat tggcttccag cgctttatcc gcttttactt tgttgagcac
     4981 ctggttattg cgctcttcat ctttgctcgg aataaacgct gccatgccgc ccatcgcaaa
     5041 agcaccgcgt ttatggcagg ttttaatcaa caggcgtgag taagcattca ggaatggttt
     5101 atccatcgtc actgcctgtc tgtctggcag gacgcgatcg ggatagtttt tcaacgtttt
     5161 gatatagctg aagatgtaat cccaacgacc gcagttcaga ccaacaatat ggtcacgcag
     5221 cgcgtgaagg atttcatcca tctggaacac ggcgggcagc gtttcaatca gcaacgtcgc
     5281 cttgatggtg ccgcgcggca gattaaagcg atcttctgca tagctgaaga cttcgctcca
     5341 ccaggccgct tcctgccagg actgggtttt cggcagatag aaatagggac cactgccctt
     5401 tgccaacagt gcctgatagt tgtggaagaa atagagcgca aaatcaaaca ggctgccggg
     5461 gattgcctca ccacgccagg tgacatgttt ttccggcaag tgcagaccgc gtacccgaca
     5521 aatcaaaacc gctggattgg gcttgagctg gtaaattttg cctgcttcat tggtgtaact
     5581 gatggtgccg ttaaccgcat cacgcaggtt aatttgcccg tcgatcactt tgttccagtc
     5641 tggtgccagt gaatcttcga aatcggccat aaagactttc acattggcgt tgagcgcgtt
     5701 gatcaccatc ttgcgctcta ccgggccagt tatctctacg cggcggtctt ctaagtccgc
     5761 aggaatcccg cgaattttcc aatcagcatc gcgaatggaa gctgtttccg aaataaaatc
     5821 aggcaacgtt ccgttatcaa tatcttgctg ctgctgaatg cgcgctgcca gaagtttatt
     5881 gcgttgtggc gtaaaatgcg tcaccagctc agtcagaaat tctaccgctt cggcagtaag
     5941 aatttgcttc tcctgctcgc catacggcct tgtgaaagcc agttcatcgg ttgttgttgc
     6001 ctgttcagtc atcgtgcagc tcctcgtcat ggatccgaaa acttccccac tgtgaacgaa
     6061 ggatcgttgt gcgcttttcg ttcagcacaa ctaagactac tcatttaaaa tgcaaaatca
     6121 aaaacaattt ccattttaat taaaaatact tgataacttg ttgataacaa tatgaataaa
     6181 atttattcac ctgctgaggt acgtttcgga aataaaaaag gcacccgaag gtgcctgagg
     6241 taaggtgctg aatcgcttaa cgatcgacta tcacagaaga ttaatccagc gttggattca
     6301 tgtgccgtag atcgtatggc gtgatctggt agacgtaata gttgagccag ttggtaaaca
     6361 gtaaattacc gtgactacgc cagctcgctc gcggtgtatt ttgcggatca ttgtgcggga
     6421 aatagttata cggtacatcc gggtctagtc cggcttccac atcgcggaaa aattcctgcg
     6481 ccagcgtttg cgcatcatat tcgggatggc ccgtcacaaa ggcaatgcgc ttatctttac
     6541 tggcaaacag atatgcatcc ccttcttccg tctctgccag aatttccaga tcggtgtaat
     6601 cacgaatcaa cgctgccgga aagtcagcat agcgcgaatg cggtgccagg aatgaatcat
     6661 caaagccacg cgtcagaagc gcatgaggat ggagaatatg atgctcgtaa acgccagaga
     6721 gtttgtcggt gcgagtttgc ttaggaatgc cgtagaggat attgagcgcg gcctgtaccg
     6781 cccagcagac aaacagcgtc gaggtgacgt gatctttcga ccactccagc acctgtttga
     6841 tctgcggcca gtaagcgaca tcattaaact ccaccaggcc cagcggcgca ccagttacaa
     6901 tcaaaccgtc aaagttctga tcctgaatat cttcaaagtt acagtagaag ttgttcagat
     6961 gctctgcggg cgtgttgcgc gattcacggg aatcgatgcg caacagctga atatcgacct
     7021 gcaaaggtga gtttgaaagc aggcgcagaa actgattttc agtttcaatc ttcttcggca
     7081 tcaggttaag gatcagaacc ttaagtggac gaatttcctg accagacgca cgagaagttg
     7141 tcatcacaaa gacgttttct tcacgcaaga aattgacggc gggtagctcg tccggcacac
     7201 gaatcggcat aacctgatta cctcactaca tacgcttata cgtttagaca tccagatagc
     7261 tgaagataac cagaaaattt gccaatgtcg agcctgcatg ttgaaggtga gaaagtttca
     7321 aggaaaagac tacgttagaa tataagaatg actaaaagga gagaaaatgg ttattagtat
     7381 tcgccgctca cggcatgagg aaggggagga actcgttgcg atttggtgtc gttctgtcga
     7441 tgccactcac gattttctat cagcagagta tcggaccgag ctggaggacc tagttcgttc
     7501 cttcctgccg gaagcgccgt tgtgggtcgc ggttaatgag cgggatcagc cggttggatt
     7561 tatgttgcta agtgggcagc atatggatgc gctgtttatc gatcctgatg tgcgcggctg
     7621 cggcgtaggt cgggtgctgg tggagcatgc gctctcgatg gcaccggaac tgacaaccaa
     7681 cgtgaatgag caaaatgagc aggcggttgg gttctataag aaggtgggtt ttaaggttac
     7741 gggacgctct gaggtggacg atttggggaa accgtatccg ttgctgaatc tggcgtatgt
     7801 gggggaataa gccgccccct atcgtgctat cgtgcctgcg taagctggat aagacgcgcc
     7861 agcgtcgcat ccggcatttt tttctgcgcc aatgcaaaaa ggccatcctg acggatggcc
     7921 tttcgcttta tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc
     7981 cgttgcttcg caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc
     8041 accgacaaac aacagataaa acaaaaggcc cagtctttcg actgagcctt ttg
//
LOCUS       DAPHBJ010000196         8033 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.198, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000196 DAPHBJ010000000
VERSION     DAPHBJ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.198"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(95..382)
                     /locus_tag="RHZ76_003809"
     CDS             complement(95..382)
                     /locus_tag="RHZ76_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HDV2814365.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(457..600)
                     /gene="mgrB"
                     /locus_tag="RHZ76_003810"
     CDS             complement(457..600)
                     /gene="mgrB"
                     /locus_tag="RHZ76_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HDV2814366.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            759..998
                     /locus_tag="RHZ76_003811"
     CDS             759..998
                     /locus_tag="RHZ76_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814367.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1141..1932)
                     /gene="kdgR"
                     /locus_tag="RHZ76_003812"
     CDS             complement(1141..1932)
                     /gene="kdgR"
                     /locus_tag="RHZ76_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HDV2814368.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLNQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2109..3482
                     /locus_tag="RHZ76_003813"
     CDS             2109..3482
                     /locus_tag="RHZ76_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2814369.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3528..4409)
                     /gene="htpX"
                     /locus_tag="RHZ76_003814"
     CDS             complement(3528..4409)
                     /gene="htpX"
                     /locus_tag="RHZ76_003814"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HDV2814370.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4601..6649)
                     /gene="prc"
                     /locus_tag="RHZ76_003815"
     CDS             complement(4601..6649)
                     /gene="prc"
                     /locus_tag="RHZ76_003815"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HDV2814371.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6669..7367)
                     /gene="proQ"
                     /locus_tag="RHZ76_003816"
     CDS             complement(6669..7367)
                     /gene="proQ"
                     /locus_tag="RHZ76_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HDV2814372.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7464..7961)
                     /gene="msrC"
                     /locus_tag="RHZ76_003817"
     CDS             complement(7464..7961)
                     /gene="msrC"
                     /locus_tag="RHZ76_003817"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HDV2814373.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
BASE COUNT         1908 a         2123 c         1832 g         2170 t
ORIGIN      
        1 ttttaatttg aagcagttgc accatggcag tgcgtataat caggatgaat tgagtaaccc
       61 ttcaacgacg gggttactca agattgagga aggattatcg ttcggcgacc agacgaataa
      121 tatcttcgtc ttcaaccgat ttttgactct caacggtctt tttatctgct ttctcaggtt
      181 cgtttgcttc acacaaacga cgtaacagtg cattttgacg tttttgctga tccagcaacg
      241 cctcaagcag ttcaatctgt tcgttagtcc gagaactggc acgattgata aaaaaccaca
      301 agatgagccc gataagaaga accaccaccg atacaaccaa agacgcaatg ttcatcacgc
      361 ctgaatttac aacttcgttc atttcaccac ctctgagtag aggcgctatt ctaccactgc
      421 tggagaggaa gaaaatctag tgctgaaaaa atgatatcac cacgggataa actggttaat
      481 ggcacaaatt ccgctgaaaa attgtacatc ctgatcgcac atcatgttga atacctgcgc
      541 ccaaagcagc aagcaagcca acaccacgac aaccagaacg acccatcgaa actttttcac
      601 tccaatctcc gcttcaccta ccttatgtca ttaaactagc atgcgttagt taaacagcgc
      661 ctaactatgt cgatattcat gctttttcgg aatgagtagc ttgcttcagc gcatattgcc
      721 gctacgatta agcgaacata aaaagagaag aggttgtaat gcgattcatc attcgcacag
      781 ttatgctgat agcactggta tggataggat tattactcag cggatatggc gttttgattg
      841 gaagcaaaga gaatgccgca ggattaggtt tgcaatgtac ctatctgact gccagaggca
      901 ccagcacggt acaatacttg cacactaaaa gtggtttctt ggggataaca gattgcccac
      961 tgttacgtaa aagcaatatc gtggtcgata atggttaagg gtaataaata accagattct
     1021 cactcatgag taaaatattc ttatagcaag cgcgtttaat ataacgcacc ttcaacttta
     1081 tttttctcat gctaaataaa gaacctgtct gtttgatatt ccgacaggtt cttactctca
     1141 tcagaacgga tagtcgtgat aacccatttg ggcagaaatt ttgcgcgctg cggtatgcaa
     1201 cattgcgaca tattcctgta aacgctcttc agagaaacgc aacgtcggga aggagatgct
     1261 caaaccggca atgaccacgc caaagcgatc aaataccggt accgcaatgc atcgcagccc
     1321 ttcttcctgc tcttcgttat cttcgccata cccctgctcg cgcacctggt tcagaacggg
     1381 taataacgct tctgtactgg tgatggtccg ctcggtactg cgtttatact ccacgccctc
     1441 aagaatttgc ttcacttcat cgcgatcgcg ccatgccagc agtaccttac caatcgcggt
     1501 gctgtacagc ggattacgac gcccaatccg tgaatacatg cgcaaattgt acatagagtc
     1561 aattttgtga atgtaaacaa tactgtcttc gtccagtgcg ccgaggtgga tagtttcttt
     1621 ggtcaggcgg gagatctcac gcatctggat atctgcgcta cgaattaaat cgacgttttg
     1681 taacgcgcga gcgcccagtt caaacaattt cagggtcagg gaatatttct ccgactcccc
     1741 ttcctgcgcc acataaccta aggttttcat ggtctgtaaa aagcgataaa cggtgctttt
     1801 tgacatcatg acgcgctgcg acagctcggt tatccctatt tcgcgctctt cacccagcgc
     1861 ctgcaaaatg ccaaaaactt ttagcacgga agatacagaa tcaggctgtt tatccagatc
     1921 tgcgttagcc atttatcacc tcattgcgag tgttttataa aaatcagaac tgttttttat
     1981 tataatttcg caccagggtg gtcgcaatcc atcttttgcc ggttagttac aattctgcga
     2041 catccaccgt gaatatcagt gctagaatca tacccctgtt gattattcac caaagatata
     2101 aaattcctat gccaaaagtt caggccgacg gcctgccatt gccccagcga tacggtgcga
     2161 tattaaccat tgtgattggt atttcgatgg ctgtccttga cggcgcaatc gccaacgtcg
     2221 ccctgccaac aatcgccacg gaccttcatg ccacgccagc cagttccatc tgggtagtga
     2281 acgcctatca aatcgccatt gtcatctccc tgctctcgtt ttcgtttctg ggcgatatgt
     2341 ttggctatcg acgtatttat aaatgcggtc tggtcgtttt tctgttgtct tcactgttct
     2401 gcgccctttc tgattcgctg caaatgctca cccttgcgcg tgtcatacaa ggtttcggcg
     2461 gtgcagcgtt gatgagcgtt aataccgcac ttatccgcct gatctatcca caacgttttc
     2521 tggggagagg gatgggcata aactcgttta ttgttgccgt ctcttctgct gccgggccga
     2581 caattgctgc agcaatcctc tccatcgcat cctggaaatg gttattttta atcaacgtac
     2641 cgttaggtat tatcgccctg cttctggcga tgcgttttct gccacccaat ggttctcgcg
     2701 ccagtaaacc ccgtttcgac ctgcccagcg ccgtgatgaa cgcgttaacc ttcggcctgc
     2761 ttatcactgc gttgagtggt ttcgctcagg ggcaatcgct aacgttaatt gctgcggaac
     2821 tggtggtaat ggttgttgtt ggtattttct ttattcgccg ccagctttct cttcccgtac
     2881 cgctgctacc ggtggattta ctgcgtatcc cgctgttttc actttctatt tgcacatctg
     2941 tttgctcttt ctgcgcacaa atgctggcaa tggtttccct gcccttttac ctgcaaaccg
     3001 tgctcgggcg tagtgaagtc gaaacaggtt tacttctgac accgtggccg ttagcaacga
     3061 tggtgatggc tccgctggca ggctatttga ttgaacgcgt acatgcagga ttgctgggtg
     3121 ctttagggtt gttcatcatg gctgcggggc ttttttccct ggttctgctg cccgcgtcac
     3181 ctgcggatat caatattatc tggccgatga tcttatgtgg cgctggattt ggcttattcc
     3241 agtcacccaa taaccacacc attattacct ccgctccgcg cgaacgtagc ggtggggcca
     3301 gtggcatgtt aggaacggct cgtctactgg gtcagagtag tggcgcggcg ctggtggcgc
     3361 tgatgctaaa tcagttcggt gataatggta cgcacgtttc gctgatggct gcggctattc
     3421 tggcagtgat tgctgcctgt gtgagtggtt tacgtatcac tcagccacga tccagggcat
     3481 aaaaaaagcg cgtcgatcag gacgcgcttt ttagtattta cttcatatta cttcaggtat
     3541 tcacccgtac gcagagcttc aattcgttta tccagcggcg ggtgagtcat gaacaactca
     3601 ctgagcgatt tcgacttacc gttaatgcag aaagccatca tgctggttgc ttcttgcggt
     3661 tcatagctgg ttttcaggcg ctgcagcgcg gcaatcattt tctcgcgacc aaccagtttt
     3721 gccgaaccgg catcagcatg gaattcacga tgacgcgaga accacatggt gataatgctc
     3781 gccagaatac caaacaccag ttccagaacc gttgcaaccg caaagtagat cagcgggttg
     3841 ccgttgctct cttcaccttc atcacgattt ccgcccataa aacccgcggc aagctgcgcc
     3901 agaatacggg aaataaagat aacgaaggtg ttcaccacgc cctgaatcag cgtcatggta
     3961 accatatcac cattggcgat gtggctgatt tcgtgagcaa ttaccgcctc ggcttcatcc
     4021 gggctcatgt tctgcagcaa accggtgctg acagcaacca gagaggcatc acggcgcgca
     4081 ccggttgcaa aagcgttgat gtccggcgca tggtagatag ccacttgcgg catagcgatc
     4141 cccgcctgac gagcctgggt tgctacagta ttgaccagcc aacgttccct ttcgttacgc
     4201 ggttgctcga tcacttcccc gccaacagat cgtaatgcca tccatttgga catcagaagc
     4261 gaaacgaagg aaccaccaaa accgaacagc aaggccatga tcatcagccc ctgaacgctg
     4321 ctcgactgta tccctgtcag gctcagtacc agcccgaaaa cgaccattac ggccaggttc
     4381 gttagcagga agagcgcgat tcgcatcata attttctttt tacctcagtt taacaaaacg
     4441 caatatgcga tacccacatc gtatgggtta cgcgactatt ttcaagtctg gatagtgcgt
     4501 aagtcaccag aaagacacaa ctttacattt tgtagcatct gatttacggc atcttgccgc
     4561 tgttaaaaaa tcaggcacaa tttcttgtgc ctgattgata ttacttgacg ggagcgggtt
     4621 gttccgcggg tctggctttt tcaagcttcg ccagatcgag tgcgatattc accgtctcat
     4681 ccagataagg atccggctcc tggtaatctt tcggtagatc atccagcttc ttcaactccg
     4741 gtttaccttc gcgtttaaag cgttcgttca aacgcgccag acgcgtcgca tcatcttcat
     4801 tattctcttt ctcacgcaca gcgtagttca gagaaacgat attgcgcttg tccttcatag
     4861 cgttgaagcg cgcgatatcc ttcatgatgt tctggaactc aggatctttc gcgatacgcg
     4921 cattatgttc cttcagcagc tccggttcaa aggcagttaa atctcctgat ttcacataag
     4981 tcgcggcatc gatgctatcc cacggcagcg cgttatcttc gaatttctca cccgtttccg
     5041 tttcttcatt acccgtcggc atgatgatgt ctggcgttac gcctttacgt tgcgtactgc
     5101 cgccgttaac gcgatagaat ttctggatcg tgtactgcac agaacccagc gctggccatt
     5161 caggacgtaa catctgatcg taaatacggt tcaatgaacg gtattgctga acggtgcctt
     5221 taccaaacgt cggttcaccc acaaccagcg cacgaccgta atcctgcatt gccgcggcaa
     5281 agatttctga agccgaagca ctgaaacggt caaccagtac caccagcggg cctttataga
     5341 acacctgccc gtcggtatcg ctgtcttcac gaaccttgcc gttgttatcg cggacctgaa
     5401 caatgggacc cgcaggaata aacagacctg agagcgatac cgcttcggtc aacgccccac
     5461 cgccattgct acgcaggtcg atgatcacac tgctgacatt ctgtttttcc agtttctgca
     5521 gttgaacttt gacatcgtct gtcaaaccca catagaagcc cggaatatcc agcacgccga
     5581 ctttctcttt accgacggtc ttcaccgaca ttttaaccgc gcggtcttcg agacgaatac
     5641 gttcacgggt caatgttaca gtgcgggtct tggtcccttt accagcaggt aaaatttcca
     5701 gacgaacttt actgcccttc ggccctttaa ttaaggcaac cacatcatca agacgccagc
     5761 caatcacgtc aaccatcggc ttgcctgttt gaccaacacc gacaattttg tcaccaacgc
     5821 tgatagcttt actcttcgct gccggaccac ctgccaccat cgaattgata acggtgtagt
     5881 catcatccat ttgcagcact gcgccaatac cttccagcga caaactcatt tcagtgttga
     5941 actgttcggt attacgcggg gaaagatagt tggtatgcgg gtcgatttca cgcgcaaacg
     6001 ccgtcattgc cagcgagaaa acatcttcgc tgttggtttg cgccagacga cgaatggcaa
     6061 atttgtagcg gcgtgtcagg gtttcacgaa tttctttatc cgtttttcct gccagcttca
     6121 ggcttaactc gtcgaatttg actttactgt cccacagcgc gttcaactca gcctcgtttt
     6181 tcggccaggg cgctttgctg cggtcaaggt tataagtgtc gttgccggtg aaatccatcg
     6241 gcttttccag taccgacaaa gcgtactggt aacgctcaaa acggcgcttt tgcgccagat
     6301 tgtagagatc gtagaaaacg tcgagtttgc ctgaacgcag ttcatcgcct aactcggttt
     6361 tctttttcgc gaactgttca acatcgcttg ccagcagcac gttgtggctg taatcgagca
     6421 gattcaggta gcggtcaaag attttggccg aaaatgcctg atcgaggtcg aactggcgat
     6481 aatgagaacg ggtgaagcgc gacgttacgc gctcacttac cgtcgcatgc tgcgtctctt
     6541 cctttaatac cggaatttga tcagcacgcg tgatatcttc tacagcgaag gtctggcctg
     6601 ctattgcaag caggccagct aacgcggtaa gcctaaaaaa catgttcatg cctggcccgg
     6661 cctccgtttc agaacaccag gtgttctgcg cgcacaatca aagacatacc cgaattcagc
     6721 tggacgcgga cgccgtcttt ggtgatttct aatacggtgg catccatcgc gttttgaccc
     6781 gctttcacct tcagggcttg tccgacagtc agagctgaaa tgtcagaaac cggggtgtgc
     6841 tgttcttcgc gaggtgcttt tactgttttt ggcgctttct ctaccggctt ttgcgcacga
     6901 ggtttacgtt cagcgccttc tttgcggcgt ggcgtggtcg gacgtggctt gcgttcgcgg
     6961 cgcggtgcgt cttctttctc accagcagct gcggcagctt cgcgtttttt cgcttgctgt
     7021 tcagcacgct gtgcctgaac acgcgctttc gcttcttcaa gctgcttgcg agcatgctct
     7081 acatgttgct cgtccagctc accgcatggg ttgccgtcaa gatcgacacg cgttgcgccg
     7141 ggtttaacac cgtaaagata acgccagctc gaagtgtaga gacgtaaagc ggatcgtaat
     7201 tgcgttttgc tcaggttcat ttccccggca acacgatcga ccaaatcctg aaaaataccg
     7261 attttcagcg gacgcgcttc accttccgca ctgaaacagt ggggaaaacg ttcggccaga
     7321 aacgcgatta cttctttact gctattcaac ttaggttgat tttccatgaa atttcctgat
     7381 tacaacggac gtagccaaca agcgcaggca tgaacaggcg tcattataat gacgctacca
     7441 gtaaatgcta cgttatccgt tgattatcct gcgacgctcg caaagaattt tttgtaatcc
     7501 gtcgttgcaa gcactttttc aagctgtgcc acaagctgac gtaagccttg ctcgtcctcg
     7561 tctgtaaagc gaccgaagac ggtactatcg atgtcaagaa caccaataat ctgatttttc
     7621 accaccagcg gcagaacaat ttcagaatta ctcgccgcat cacaggcaat atgcccgtca
     7681 aacgcgtgaa catcctcgat acgctgcact tgattgcggg caaccgcagt gccgcacacc
     7741 ccgcgcccga cgggtatccg gacacaggca attttgccct gaaatggtcc gagtaccagt
     7801 gtatcgtcct caagcaaata aaaacctgcc cagtttacgt cagtgagacg ctcgtataac
     7861 aacgcactgg tgttcgcaag cgttgccaga aaactggttt ctcccgccat cagcgcgtta
     7921 aagtcgcgat ttaaatccgc gtaaaattct gttttgttca ttatataatc acttggttgt
     7981 cttacctgga actgccagcc tattaaaata agcattaaat gcgttaatgc tca
//
LOCUS       DAPHBJ010000197         8002 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.199, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000197 DAPHBJ010000000
VERSION     DAPHBJ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.199"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..737)
                     /locus_tag="RHZ76_003818"
     CDS             complement(<1..737)
                     /locus_tag="RHZ76_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HDV2814374.1"
                     /translation="MFKARICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSP"
     gene            complement(801..1661)
                     /gene="pdxI"
                     /locus_tag="RHZ76_003819"
     CDS             complement(801..1661)
                     /gene="pdxI"
                     /locus_tag="RHZ76_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HDV2814375.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALAHDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(1869..7931)
                     /locus_tag="RHZ76_003820"
     CDS             complement(1869..7931)
                     /locus_tag="RHZ76_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDV2814376.1"
                     /translation="MQRKTLLSACIALALSGQGWAADITEVETTTGEKKNTNVTCPAD
                     PGKLSPEELKRLPSECSPLVEQNLMPWLATGAAALITALAVVELNDDDDHHHRNNSPL
                     PPTPPDDESDDTPVPPSPGGDEIIPDDPDDTPTPPKPISFNNDVILDKAEKTLTIRDS
                     VFTYTENADGTISLQDSNGRKATINLWQIDEANNTVALEGVSADGATKWQYNHKGELV
                     ITGDNATVNNNGKTTVDGKDSTGTEINCNNGKVIQDGDLDVSGGGHGIDITGDSATVD
                     NKGTMTVTDPESIGIQIDGDKAVVNNEGESTITNGGTGTQINGDDATANNNGKTTVDG
                     KDSTGTEINGNNGKVIQDGDLDVSGGGHGIDITGDSATVDNKGTMTVTDPESIGIQVD
                     GDQAVVNNEGESAITNGGTGTQINGDDATANNNGKTTVDGKDSTGTEIAGNNGKVIQD
                     GDLDVSGGGHGIDITGDSATVDNKGTMTVTDPESIGIQVDGDQAIVNNEGESTITNGG
                     TGTQINGNDATANNSGKTTVDGKDSTGTKIAGNIGIVNLDGSLTVTGGAHGVENIGDN
                     GTVNNKGDIVVSDTGSIGVLINGEGATVSNTGDVNVSNEATGFSITTNSGKVSLAGSM
                     QVGDFSTGVDLNGNNNSVTLAAKDLKVVGQKATGINVSGDANTVNITGNVLVDKDKTA
                     DNAAEYFFDPSVGINVYGSDNNVTLDGKLTVVSDSEVTSRQSNLFDGSAEKTSGLVVI
                     GDGNTVNMNGGLELIGEKNALADGSQVASLRTGYSYTSVIVVSGESSVYLNGDTTISG
                     EFPLGFAGVIRVQDKALLEIGSGATLTMQDIDSFEHHGTRTPELTYADSGAKIVNKGT
                     VEIQNLGFAFVTGENTTGINSGTISLLQNGKDPAPSPIVLLATNGGSATNAGTITGKV
                     TERHSVFNKYSTGTSNSFIFNNDVSSITGLVAQSNSTIINTDSGIIDLYGRGSVGMLA
                     IADSTAENQGKITLDSMWVDANDTTAMRDIASNSAIDFGTGVGVGTDSYSGAGKNATA
                     INQLGGVITIYNAGAGMAAYGASNTVINQGTINLEKNGNYDDSLAANTLVGMAVYEHG
                     TAINDQTGVININVGTSQAFYNDGTGTIVNYGTICTFGVCQSGNEYNNTDDFTSLIYT
                     GGDTITRSGETVTLNKSAAVTDKLAGNVVNSGTLSGDQIMVSSGLLENTSGGIINNLV
                     TLAKGAVIKNTGVMTNNVDVSGGTLNNAGEMTAQITMNAGADSSLVNNTGTINKIVQN
                     AGVFNNSGSVTGRMMSAGGVFNNQTDGAIMRGAALTGTAVANNEGTWNLGSSSEGNNT
                     GMLEVNNNSAFNNRGEFILDNDKNAVHINQSGTLYNTGHMNISNSSHNGAVNMWGGNG
                     RFINDGTIDVSAKSLVVSANNAGDQNAFFWNQDNGVINFDHDSASAVKVTHSNFIAQN
                     DGIMNISGTGAVAMEGDKNAQLVNNGTINLGTAGTTDTDMIGMQLNANATADAVIENN
                     GTINIFANNSFAFSVLGTVGHVVNNGTVVIADGVTGSGLIKQGDSINVEGMNGNNGNS
                     SEVHYGDYTLPDVPKPNTVSVTSGSDEAGGSMNNLNGYVVGTNVNGSAGKLKVNNASM
                     NGVEINTGFTAGTADTIVSFDNVVEGSNLTDADAITSTSVVWTAKGSTDASGNVDVTM
                     SKNAYTDVATDASVNDIAKALDAGYTNNELFTSLNVGTTAELNSALKQVSGSQATTVF
                     REARVLSNRFSMLADAAPKVGNGLAFNVVAKGDPRAELGNNTEYDMLALRKTIDLSES
                     QTMSLEYGIARLDGDGAQKAGDNGVTGGYSQFFGLKHQMSFDNGMNWNNALRYDVHNL
                     DSSRSIAFGNTNKTADTDVKQQYLEFRSEGAKTFEPSEGLKVTPYAGVKLRHTLEGGY
                     QERNAGDFNLNMNSGSETAVDSIVGLKLDYAGKDGWSASATLEGGPNLSYAKSQRTAS
                     LAGAGSQHFNVDDGQKGGGINSLTSVGVKYSSKESSLNLDAYNWKEDGISDKGVMLNF
                     KKTF"
BASE COUNT         1888 a         2152 c         1737 g         2225 t
ORIGIN      
        1 tcaggagaag gcttagggcg aaacagcgct tcttttttca tctgattaca gacattccct
       61 gccatgcgaa tattgcttcg cttaaaaatt tctttcagag catcttcatc atttattaga
      121 ccccaataat atgcatcagg atttattatc atcagcgctt ttttgacatt ttcagtgtca
      181 tttttgtcac tcgcacggac catgaattca ctatttgtcg aagacgcaac aacaggtgca
      241 attatgtcat tgataaatat cccacgcgca ttaatactcc ccttaatcgt agacggatta
      301 tcgtaatatg agtcgataaa taccagtcca cttgcattta tcgaaccggt aattaacagt
      361 ggtgaatccg taactatatt cgttgccgtc acgttaccga gaataaccca tggattagaa
      421 agagagaaag tggaaatatt acctttgact gtcaaattac caagaacaat caatttttga
      481 ttactctcta tattaccttc taccactaaa tcgccacgat gcactttcca gtccgatgca
      541 gataaaccga aggggacaag attcacgcta ctgttgatag cttgttcaac ttgttgttta
      601 tcttcagcgg tagccgcaga aaaaaatggt tcaatatcaa ctgtatttgc aacacatcga
      661 agttccgcct gtactggaag actcagcata aacaggggaa gtaaacctat ccagccgcaa
      721 atcctggcct taaacatatg cactccatgc cattaaaaaa gaaacaaaag ggaaatgcat
      781 ctttcccttt tgtatattcg ttattctcgc gaaataccat ccaacgtaga caacacttcc
      841 tcagaaagat gcaatttttc agcagccata ttctcccgta aatgcgcaac cgaagacgtc
      901 cctgggatca gcaaaatatt cggtgaacgt tgtaacagcc acgccagcgc cacctgcatg
      961 ggtgttgcac ccaggctcgc agcaacatcg gaaagtgtgg acgattgcag cggtgtaaag
     1021 cccccgagcg ggaagaacgg cacgtaggca atgccatcgt gggccaaagc atcaatcatt
     1081 gcatcatcag cacggtgcgc gatgttgtat tcgttttgca cacagacaat ttcggcaatc
     1141 ttgcgcgcct ctgcaacctg cgtcggtgtg acgttgctca ggccaatatg ttttaccaag
     1201 ccttgttgtt gcatctctgc cagcacggtc aggctggcct caatcgatcc ttccgctggg
     1261 ccatgaccat cccccatcat aacgcgcagg ttaaccacat ccagcacgtc cagcccgaga
     1321 ttacgtagat tatcgtgcac cgctttttgc agctctgccg gagaaaatgc gggcaaccag
     1381 gatgcgtcct ctccacgccg cgcaccaatt ttagtgacaa ttgtcaggtc gtcagagtaa
     1441 ggataaagcg cttcgcggat aatctgattg gtgacgtgcg gaccataaaa gtcgctggta
     1501 tcaatatgat tgacgcccaa tgccagcgcc tcacgcagca cggttatagc gacgtggcga
     1561 tctcgtgggg ggccaaaaac tccaggacct gccagttgca tcgcgccata accaagacgg
     1621 ttaacggatt ttgtaccgag agtaaatgta ttgctgctca tcgcaccctg ccctgtaata
     1681 aaacggatta agaaaaatca gtatagaaac gaaaagagat aaataatgcg aaggtgtaaa
     1741 caagattact ggatgagttg ctctattgtc ctgaaagaca caaaacaaaa cgcgtcacgt
     1801 accacatccg gcaataaaaa aaccgcaacc tgtatcagcg ttgcggttta gggatcacat
     1861 gctaaaaatt agaacgtttt cttgaagttc agcatcacgc ctttatcgct gatgccatcc
     1921 tctttccagt tgtacgcatc cagattcagc gaactttctt tgctgctgta cttcacgccg
     1981 acgcttgtca ggctattgat gccgccgccc ttctgaccgt catcgacgtt aaagtgctga
     2041 ctacctgcgc ctgccaggct tgccgtacgc tggctcttcg cgtagctcag gttcggcccg
     2101 ccttccagcg tagcgctagc gctccagccg tctttacctg cgtagtccag tttcagcccg
     2161 acgatgctgt ccaccgccgt ttcgctgcca ctgttcatat tcaggttaaa gtctccggca
     2221 ttgcgctcct gatagccacc ttccagtgtg tgacgcagtt ttacacccgc atatggcgta
     2281 accttcagtc cttcgctcgg ttcgaaagtc ttcgcccctt cgctgcggaa ctccaggtac
     2341 tgctgtttca cgtcggtatc agccgttttg ttcgtgttgc caaatgcaat cgagcggctg
     2401 ctgtcaaggt tgtgaacgtc gtaacgcaag gcgttattcc agttcatgcc gttatcgaac
     2461 gacatctgat gtttcaggcc aaagaactgg ctataaccgc ctgtaacgcc attatcaccc
     2521 gctttctgcg caccatcacc atcgagacga gcgataccgt actccagact catcgtctgg
     2581 ctttcgctca ggtcgatagt tttacgcaat gccagcatgt cgtattcggt attattacct
     2641 aactcggcac gcggatcgcc tttcgcgaca acgttgaacg ccagaccgtt acccactttc
     2701 ggcgcggcat ctgccagcat actaaagcgg ttgcttaaca cgcgcgcttc gcggaatacc
     2761 gtggtcgcct ggctaccgct gacctgtttc agagcactgt tcagttcagc agtcgtgccg
     2821 acgttcaggc tggtaaacag ttcgttgttg gtgtaacccg catccagtgc tttcgcgatg
     2881 tcattcaccg aggcatctgt tgccacatcg gtataagcgt ttttgctcat ggtgacgtca
     2941 acgttaccgc tggcatcggt gctgcctttg gcagtccata ccacggacgt tgaggtgatg
     3001 gcgtcagcgt cggtcaggtt gctaccttcc actacgttat caaaactcac aatggtgtct
     3061 gccgtaccag cggtaaagcc cgtgttaatc tccacgccgt tcatgctggc attgttaacc
     3121 ttcagcttcc cggcgctgcc gttaacgttg gtaccgacga catagccgtt gaggttgttc
     3181 atgctgccac cagcctcatc acttcccgac gttacactaa ccgtattggg cttcggcaca
     3241 tccggcaacg tatagtcgcc ataatgcact tcgctgctat taccgttgtt accgttcata
     3301 ccttcaacat tgatgctgtc gccctgcttg atcaggccag aacccgtaac cccatcggca
     3361 atcaccaccg tgccgttgtt aaccacatga cctactgtac ccagtacgct aaatgcaaac
     3421 gagttattag cgaagatatt gatggtgccg ttgttctcga tcaccgcatc cgccgtggcg
     3481 ttggcattga gttgcatacc gatcatatcc gtgtcagtag tgcctgcggt accgaggttg
     3541 atggtgccat tgttaaccag ctgcgcgttc ttatcacctt ccatagccac agcaccggtg
     3601 ccgctgatgt tcatgatgcc gtcattctgg gcaataaagt tgctgtgggt gactttcacg
     3661 gcactggcgc tgtcgtgatc gaagttgatg accccgttat cctggttcca gaagaaggca
     3721 ttctgatcgc cggcattatt agcgctgact accagtgact tcgcagaaac atcaatcgtt
     3781 ccgtcattga taaaacgacc atttccgccc cacatattaa cggctccgtt gtgggaagaa
     3841 ttactgatgt tcatgtgacc ggtattataa agcgtaccgg actggttgat gtgcacagca
     3901 ttcttgtcgt tatcaagaat aaactcgccg cggttattga aagcagaatt attattaact
     3961 tccagcatcc cggtgttgtt accctcactg ctacttccga ggttccaggt tccttcgtta
     4021 tttgccactg cagtacctgt cagcgcagca cctctcataa tcgccccgtc agtttgatta
     4081 ttaaagaccc cgccagccga catcatccgc cctgttacac tgccactatt attgaatacc
     4141 cccgcgttct gcacgatttt attgatagtt ccggtgttgt tcactaacga actatcagca
     4201 ccagcattca tggtaatttg cgcagtcatt tctccggcat tattgagggt tccaccgcta
     4261 acatcgacgt tattcgtcat caccccggta tttttaatga cggccccctt ggcaagtgtt
     4321 actaagttat tgatgatgcc accgctggta ttttccagaa gaccgcttga taccataatt
     4381 tgatcaccgg aaagcgttcc gctgttaaca acattcccag ccagcttatc agtcacagca
     4441 gcagatttat ttagcgttac agtttctccg cttcgtgtaa tcgtatcgcc accggtatag
     4501 atcagtgagg tgaaatcatc tgtgttattg tactcattcc ccgattggca cacgccgaaa
     4561 gtgcagattg taccatagtt aacaattgtt cctgtgccat cgttataaaa cgcctgacta
     4621 gtaccaacat tgatattgat aacacccgtc tggtcgttga tagcagtacc atgctcataa
     4681 acagccatcc ctaccagagt atttgctgcc agactatcgt cataattacc atttttttcg
     4741 aggttaatcg tcccctggtt aataactgta ttgctggcgc cataggccgc catacctgcg
     4801 ccggcgttat aaatagttat aacaccgccc aattggttaa ttgctgttgc atttttccct
     4861 gcaccactat aactatcagt accaactccc acacctgtac cgaagtcaat ggcgctgttg
     4921 ctagctatat ctcgcattgc ggtagtgtca tttgcatcta cccacataga atccagtgta
     4981 attttaccct gattttctgc tgttgaatct gctatagcaa gcatgccgac actaccacga
     5041 ccatacaaat caatgatgcc gctgtcagta ttgataattg tgctattcga ttgagcgact
     5101 aaccctgtta tgctactgac atcgttatta aaaataaatg aattcgatgt gcccgttgaa
     5161 tacttgttaa atacgctatg tcgttccgtc actttacctg tgatcgtacc tgcattagtg
     5221 gcgctccctc cgttagtagc cagtaaaaca atgggagacg gtgccggatc tttaccattt
     5281 tgtaataacg agatcgtgcc actatttata cctgttgtat tttcaccagt aacaaaagca
     5341 aaacctaaat tctgaatttc aacagtacct ttattaacaa ttttcgcacc ggaatcagca
     5401 taagtaagtt ctggggttct tgtcccatga tgttcaaaac tgtcaatatc ctgcattgtt
     5461 agcgtagcgc cactgccaat ttccagcaaa gctttatcct gtacccgaat aaccccggca
     5521 aaccccagag ggaattctcc gctgattgtc gtatctccat tcagatatac cgacgactca
     5581 ccactaacga caataacgct ggtataacta tatcctgtgc gcaaggaagc aacctgcgac
     5641 ccatctgcaa gcgcgttttt ctctccaatc agttcaagtc caccattcat attaacggta
     5701 ttgccatcgc caatcacaac cagacctgac gttttctctg cgctgccatc aaataaatta
     5761 ctctgacgag aagtaacctc actgtctgat acaacagtta actttccatc cagcgtcacg
     5821 ttattatcac tgccgtaaac gttgataccc acggatggat cgaagaaata ttccgccgca
     5881 ttgtctgcgg ttttatcctt atcaaccaga acgttaccag tgatattcac tgtattcgca
     5941 tcgccagaaa cgtttatgcc cgtcgctttc tgcccgacca cttttagatc ttttgccgcc
     6001 agcgtcacgc tgttattgtt gccattaaga tctaccccgg tcgagaaatc gccaacctgc
     6061 atactgcctg ccagcgaaac cttcccactg ttggttgtga tgctgaaccc tgtcgcttca
     6121 ttgctaacgt taacatcacc cgtattggat actgttgccc cctcaccgtt gatgagcacg
     6181 ccaatcgatc cagtatcgga aacaacaata tctcctttgt tgttaaccgt gccgttgtca
     6241 ccaatgttct caacaccatg cgcaccgcct gtaacagtca ggctaccatc cagatttaca
     6301 atgccgatat tgcccgcgat tttggtaccc gtggaatctt ttccatcaac agtggttttt
     6361 ccactgttat tcgcggtggc gtcgttaccg ttgatctgag tgccggtgcc gccattggtg
     6421 atagtgctct cgccttcgtt attgacgatg gcctggtcgc cgtcaacctg gataccgatg
     6481 gactccggat cggtgacggt catggtgccc ttgttatcca ccgttgcgct gtcgccggtg
     6541 atatcaatac cgtgaccgcc gccgctgaca tccagatcgc cgtcctgaat caccttcccg
     6601 ttattgccag caatttccgt accggtggaa tccttgccgt caacggtggt tttgccgttg
     6661 ttgtttgccg tggcgtcatc accgttaatc tgcgtgccgg tgccaccgtt ggtgatggcg
     6721 ctctcgcctt cgttgttaac aaccgcctgg tcgccgtcaa cctggatacc gatggactcc
     6781 ggatcggtga cggtcatggt gcccttgtta tccaccgtcg cgctgtcgcc ggtgatatca
     6841 atcccgtgac cgccgccgct gacatccaga tcgccgtcct gaataacttt tccgttatta
     6901 ccgttgattt ccgtaccggt ggaatccttg ccgtcaacgg tggttttgcc gttgttgttt
     6961 gccgtggcgt catcaccatt aatctgggtg ccggtgccgc cgttggtgat ggtgctctcg
     7021 ccttcgttat taacaaccgc cttgtcgccg tcaatctgga taccgatgga ctccggatcg
     7081 gtaacggtca tggtgccctt gttatctacc gtcgcgctgt caccggtgat atcaataccg
     7141 tgaccgccgc cgctgacatc cagatcgccg tcctgaatca ctttcccgtt attacagttg
     7201 atttccgtac cggtggaatc cttgccgtca acggtggttt tgccattgtt gtttactgtg
     7261 gcattatcac ccgtaataac aagctcacct ttatgattat attgccactt cgttgcgcca
     7321 tctgcgctca ccccttcaag ggcaacagtg ttattcgctt catcaatctg ccaaagatta
     7381 atcgttgcct tacgaccatt gctatcttgc agagaaatag tcccgtcagc attctcggta
     7441 taagtaaaaa ctgaatcgcg aatagttaac gttttttctg ctttatcgag aataacgtca
     7501 ttattaaacg aaatcggttt gggaggtgta ggcgtatcat ccggatcgtc cggtattatc
     7561 tcatctccgc caggacttgg gggaactgga gtgtcgtctg attcatcatc agggggtgtc
     7621 ggtgggagtg gagaattgtt gcgatgatga tgatcatcat cgtcgtttag ttccactacg
     7681 gctaaggccg tgattaacgc agcagcgcct gtggcaagcc atggcatcag gttttgttcg
     7741 actaaaggag agcattcaga gggtaagcgt ttaagctctt ccggactgag ttttcctggg
     7801 tctgccggac aagtcacatt ggtatttttc ttttcacctg tggtggtttc tacctctgtg
     7861 atatctgccg cccaaccctg accactcaga gctaatgcaa tacaggccga caatagagtt
     7921 ttcctttgca taataaatcc ttctggcgaa tataaagaaa gccggatgca ctaacccgac
     7981 caattagtta tttaaaacat tg
//
LOCUS       DAPHBJ010000198         7999 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.200, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000198 DAPHBJ010000000
VERSION     DAPHBJ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.200"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            70..480
                     /locus_tag="RHZ76_003821"
     CDS             70..480
                     /locus_tag="RHZ76_003821"
                     /inference="COORDINATES: protein motif:HMM:NF012354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2814377.1"
                     /translation="MRHDFNDYVAFMAVAQECSFSRAAVRLGISASTLSHTIRRLEER
                     VGMPLLMRTTRRVAVTEAGKQLLLTLEPGLARIEAGVTALQRLRHTPAGLVRLTVSDH
                     MIHECLWPRLSPLLKKIFGYQGGNVAAERFCRHC"
     gene            446..1006
                     /locus_tag="RHZ76_003822"
     CDS             446..1006
                     /locus_tag="RHZ76_003822"
                     /inference="COORDINATES: protein motif:HMM:NF015431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814378.1"
                     /translation="MSQQNGFVDIVEGNFDAGVRIGDDILKDMVAVRIAPDIRFVVIG
                     SSTYLAERGLPLHPSQLEKHDCINIRLSPTGPLYGWEFVRQGENIVKKVDGQLTFSSI
                     YPMLHAVKDGYGLAYLPESSALAGIEAGHYCRVLEDWSEAFPADMIIPIMSITGSGKD
                     NTDTLHEKGKSNITCCAPDFKNERFT"
     gene            1089..1964
                     /locus_tag="RHZ76_003823"
     CDS             1089..1964
                     /locus_tag="RHZ76_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDV2814379.1"
                     /translation="MTSQLNWQAPAINRKKVGDMTVTMLSDGYLDVSFELLSGIDGSR
                     AEDLLQKRGVPAVPRININVYVIQTPERTILIDSGAGGINGWGGRLQVALAAAGIDPL
                     QIDTLLLTHAHPDHIGGLAGPLQTPVFRNVQQLFVHEKELAFWRDETISAGAPDAFKP
                     FFEVARNAFSAYDDKLVPFREEAILPGIQTVPLFGHTPGHTGYLLGDEKESLLIWGDI
                     VHFPHIQVAQPDVTIAFDSDPAAAAAIRSKVLDRAASDNLAVSGMHFNLPTTGKVIRE
                     GNSFALNYDLWSPAV"
     gene            2074..2925
                     /locus_tag="RHZ76_003824"
     CDS             2074..2925
                     /locus_tag="RHZ76_003824"
                     /inference="COORDINATES: protein motif:HMM:NF013088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV2814380.1"
                     /translation="MAHYSCALAFCIPLESGFVAAKFTVQYSAPFHLLFLRGAFSCLV
                     LLLLTLGFRARFPSGRGMVRNLIVGLFLQSFFLGGCFYAIHRGMPSGIAALITGLQPV
                     LTAILVSLMHRQSLHPRKWLGIAIGFAGVFLVLSPGQHSFALDPVAFGSVMLGLFGVT
                     IGALYQRRAQGDGHIFANTFLQYVTLTISMGAISLMFESAPTQWSLSFALGLGWLVIG
                     VSVLAIMLLIFMIRSGEATKVATYFYLVPILTAAEAWLLFGEAITLPMLVGMAVTIIG
                     LIFVIRE"
     gene            2995..3837
                     /locus_tag="RHZ76_003825"
     CDS             2995..3837
                     /locus_tag="RHZ76_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2814381.1"
                     /translation="MNMQTVSFSDSPVDDLLAELLTLARSRFPLPCDCLRAEQTHQVK
                     KFEVEGATLSFPLEGEFRYRENEKWHRVIPGEIMVVPNARSIDIEYAPSEQSGEFVAL
                     SVVLAEEQLEAARLLLAAPPPQDTGSIAAISVSALLSPLNRWTAAMREGKRPLSLHAM
                     VEVVLWLYGMGYHALLRARQPSLAMKIRQMVADKPAHPWSAAEIEDLTGLSGPTLRRH
                     LASESTSLRSVIADARIAEALRLLMTSTLPVKTVAGRVGYNSVSSFSRQFSERYGIEP
                     SGFR"
     gene            4132..4791
                     /locus_tag="RHZ76_003826"
     CDS             4132..4791
                     /locus_tag="RHZ76_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV2814382.1"
                     /translation="MVHVFIIGGAGKIGQRLCRILSGRGHAVRALYRKPEQQALLGEL
                     GAEPVSGSLTELDAAALAMHFAGSDTVVFTAGAGGKGGQEMTNAVDGEGLKKSVAAAL
                     SAGVSRFLLVSAFPESARGETTSESFENYMRVKKMADVELAQSELDWVILRPGALQDK
                     TGTGYVRAGLAIPYGNVPRDDVAATLAELIEQPAVSRVIIELTSGDAPVREAIQKLAG
                     R"
     gene            complement(4833..5486)
                     /locus_tag="RHZ76_003827"
                     /pseudo
     CDS             complement(4833..5486)
                     /locus_tag="RHZ76_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5894..6220)
                     /locus_tag="RHZ76_003828"
     CDS             complement(5894..6220)
                     /locus_tag="RHZ76_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002437303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HDV2814383.1"
                     /translation="MLDEYKKCIEACYLCATACDNCAASCLDEENLEMMRECIKLDMQ
                     CANICRLAAQFMTLNSGSAQDLCRLCADVCQKCGDECGKHEHDHCQDCSSACHHCAEQ
                     CRKMAA"
     gene            6652..7785
                     /locus_tag="RHZ76_003829"
     CDS             6652..7785
                     /locus_tag="RHZ76_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-independent formaldehyde
                     dehydrogenase"
                     /protein_id="HDV2814384.1"
                     /translation="MKAVIYNGPYDVKVKDVPDAKIVRPTDVLVRITTTNICGSDLHM
                     YEGRTSFEQGRILGHENLGEVVEIGSGVERIKVGDYVCLPFNVGCGFCENCEKGLTGF
                     CLTTNPGSAGAAYGFAEMGPWEGGQAELLRVPFADFNCLVLPPDAAEKEDDYVMLSDI
                     FPTGWHATELAGLKPGESVAIYGAGPVGLMAAHSAIIKGASQVFVVDTHTDRLALAEK
                     MGATGINATGDEAVQKILDLTDGRGTDCGCECVGYQCCNKHGHEDNSVTMNSLVASTK
                     ATGGIGVVGVFIPQDPGAESELAREGKMPFDFGSFWFKGQAIRTGQANVKAYNRQLSR
                     LIHHDKASPGKIISHRLSLEEAPAGYKHFDERDEGWTKVILKP"
BASE COUNT         1832 a         1999 c         2115 g         2053 t
ORIGIN      
        1 ctcacagaat cggcatcatg aacctaaatg gttctgataa tgttatcgga gatcctgaac
       61 gggaacggta tgcgtcacga ttttaatgac tatgttgcat tcatggcagt agcacaggag
      121 tgcagttttt cacgggctgc tgtccgcctc gggatttcgg catccacgct ttcacacacg
      181 atacgtcgtc tcgaggagcg cgttggcatg ccgttactta tgcgtacaac caggcgggtg
      241 gctgtcaccg aagccggtaa acaattatta ctgacgcttg aacccggtct tgcgcgcatt
      301 gaagcgggcg tgacagctct tcagagactg cgccatacac cggctgggct ggtcagactt
      361 acggtatcag atcacatgat tcatgaatgt ctctggccac gattgtcacc gttactaaaa
      421 aaaatattcg gatatcaagg tggaaatgtc gcagcagaac ggttttgtcg acattgttga
      481 gggcaatttt gatgccggag tccgaattgg tgatgacata ttaaaggata tggtggccgt
      541 ccgaatcgca ccggacatac ggtttgtcgt cattggatcg tccacctatc ttgcagaaag
      601 aggtctgccg ctacatccct ctcagctgga aaaacatgac tgcatcaata tacgtctgtc
      661 acccacaggc ccgctgtatg gctgggaatt tgtcaggcag ggggagaata ttgtgaaaaa
      721 agtggacggg cagcttactt tttcaagcat ttatccaatg cttcatgcag taaaagatgg
      781 ctacggcctg gcctacctgc cggagtcttc tgccctggcg gggattgagg cgggacacta
      841 ttgtcgggtg cttgaggact ggtcagaggc ttttcccgcc gacatgataa ttccaataat
      901 gtccattaca ggctccggta aagataatac cgacacctta catgagaaag gcaaaagcaa
      961 tataacctgc tgtgctccgg attttaagaa tgagcggttt acgtagcagg atgagcagtt
     1021 cgaaccagaa atttcgttac tattgctaaa gtaaaacctg atgacctcta atcgtaacgg
     1081 agaccaatat gacttcacaa ctgaactggc aggccccggc tatcaatcgc aaaaaagtag
     1141 gtgacatgac tgtgaccatg ctgagtgatg gctatctgga cgtatctttt gagctgctgt
     1201 ctggtattga cggctcacgt gccgaggatt tactgcagaa gcgcggtgtg ccagcagtac
     1261 cccgtattaa tatcaacgtc tacgttatcc agacgcctga gcgaaccatc ctgattgaca
     1321 gtggtgccgg aggtattaat ggctggggcg gtcggcttca ggtagccctg gccgcggcag
     1381 gcattgaccc gcttcagatt gatacccttc tcctgactca cgcgcatcct gaccatatcg
     1441 gcgggcttgc aggccccctg cagactcccg ttttccgcaa tgttcagcag ttgtttgtgc
     1501 atgagaaaga actcgctttc tggcgtgatg aaaccatttc cgctggcgcg cctgacgcat
     1561 tcaagccgtt ctttgaagta gcaaggaacg ccttcagcgc ttatgacgac aaactggtgc
     1621 cattccgcga ggaagcgatt ttaccaggta ttcagactgt accgctgttc ggccatacgc
     1681 caggacatac cggttatctg ctgggtgatg aaaaagagtc gctgctcatc tggggtgata
     1741 ttgttcactt cccacacatt caggttgctc agcctgacgt tactatcgcg ttcgacagcg
     1801 atcctgcggc ggcggcggcc atccgtagca aagtgcttga ccgcgcagcg agcgataacc
     1861 tggctgtcag tggtatgcac ttcaacctgc ctactaccgg taaggttatc cgtgaaggta
     1921 acagcttcgc actgaattac gacctgtggt cacccgcagt ctgatcacct tccttcccgg
     1981 tgcacacaat agtgaccggg aagagatccc ctttcatatc gcccgacaag aacccacctg
     2041 caatactatg aatatcactg cctcacaaaa aaaatggctc attactcttg cgccctggct
     2101 ttttgtattc ctctggagtc agggtttgtc gcagccaaat ttaccgtgca gtacagcgcg
     2161 ccgtttcatc ttttattcct gcgtggcgca ttttcctgtc tggtccttct gctgcttaca
     2221 ctggggttca gggcccgttt tccgtccggc agaggaatgg ttcgcaacct gattgtcgga
     2281 cttttcctgc agagcttctt tctgggcgga tgcttttatg ccatccatcg tggcatgccg
     2341 tcaggcattg cggcgctcat caccggcctg cagccggtac tgactgcaat actggtttca
     2401 ctcatgcacc gtcagtccct gcatccccgg aaatggctgg gtattgcgat tggctttgcg
     2461 ggcgttttct tggtgctatc gccagggcag catagttttg cactggatcc tgtggctttt
     2521 gggtcggtca tgctggggtt atttggcgta acgatcggag cgctttatca gcgccgtgct
     2581 cagggagatg gacatatctt tgcgaacacg tttttacagt acgtcacttt gacaatatcg
     2641 atgggtgcaa ttagcctgat gttcgaatct gctccgacgc agtggagctt atcatttgcg
     2701 ctgggtctgg gctggctggt tattggcgta tcggttctgg cgattatgtt gctgattttt
     2761 atgatacgaa gcggtgaagc aaccaaagtc gctacttact tttacctggt tcccattctc
     2821 acggcagctg aagcgtggtt gctgtttgga gaggccatta cattgcctat gcttgtgggt
     2881 atggcagtaa ctataatcgg cctgattttt gtcatccggg aatagacaac cgcacgcgtc
     2941 gctgtatgct tttgtatatg gcgacgtata atagcggtca aagtcaaatt ccggatgaat
     3001 atgcaaacag tcagcttttc cgattctccc gttgatgatc tcctcgctga gcttcttacc
     3061 ctggcacgct cccgttttcc acttccctgc gattgccttc gcgcagaaca gacgcatcag
     3121 gtaaagaagt tcgaggtgga aggtgcgacc ctttccttcc cgctggaagg agaattccgg
     3181 tatcgtgaaa atgagaaatg gcaccgggtg atcccgggcg agattatggt cgtcccgaat
     3241 gcccgtagca tcgatataga atatgcccct tcagaacaaa gtggtgagtt tgtcgccctg
     3301 agcgtggtgc tcgcggagga acagcttgaa gcagccaggc tgttacttgc cgcaccacca
     3361 ccacaggaca ccggcagcat cgcagccatt tccgtatcag ctcttctttc accccttaac
     3421 cgctggacag ccgcaatgcg ggaagggaag cgtccgcttt ccctgcacgc catggttgaa
     3481 gtggtgctct ggctttatgg catgggatac catgctttgc tgcgagcccg gcagccaagt
     3541 ctggccatga aaatccgtca gatggtagcg gacaaaccgg ctcatccctg gagtgctgct
     3601 gaaattgaag atctgacggg actcagtggc cccacgcttc ggcgccacct ggccagtgag
     3661 tcaaccagct tacgctcagt tatcgccgat gcacgtatcg cggaggctct ccgcctgctg
     3721 atgacatcga cgctgccggt aaaaacggtc gcggggcggg tgggttacaa ttcagtatcg
     3781 agcttcagca ggcagttttc tgaacgctat ggaatcgaac cttcagggtt ccgctgactt
     3841 cagttcgttc aaggtacaca ttgcgtccct cgcacttcag taacttgcgt ggatctttcg
     3901 aatataacgg aagatattgg ccggcattgc gtgtaaccgg ttcaggagtt ctcatcgtga
     3961 cgggagagct aaatcggtac ggctgttgtt ctcagctata agaacaactg agtatctgac
     4021 cagctgtctg gtgctggcag cagcatgcag tgcaatcggt caggctcaaa ggaactgacc
     4081 tgatgtcagg ttataatttc ggacacatca taaccccaga aggagacaac tgtggttcat
     4141 gttttcatta tcggtggtgc aggcaaaatc ggccagcgtc tgtgcaggat cctttcagga
     4201 agggggcatg ctgttcgtgc cctctaccgt aagccagagc agcaggcatt gctaggcgag
     4261 ctgggtgctg agcccgtcag tgggagcctg acagagctgg atgcagctgc gctggcgatg
     4321 cactttgcag gtagtgatac agtggtgttt actgccggtg ccggcggtaa gggggggcag
     4381 gagatgacca atgcggtcga cggcgaaggg ctgaaaaagt cggtagctgc cgccctcagt
     4441 gccggtgtgt ctcgctttct gctcgtttct gcattcccgg agtccgcacg gggagaaacc
     4501 acttctgaat cattcgaaaa ttatatgcgt gttaaaaaaa tggccgatgt ggaactagca
     4561 caaagcgagc ttgactgggt gatcctgcgc cccggcgcgc tgcaggataa gactggtacg
     4621 ggttatgtac gtgccggcct ggccatcccg tatggtaacg tgccccgtga tgacgtggcg
     4681 gctacgctgg ctgaactcat tgagcaacct gccgtcagcc gcgtcatcat tgagctgacg
     4741 tctggcgatg ctcctgtccg tgaagccata cagaagctgg cgggccgata aaaggcagcg
     4801 cacgtaccag caggctgcag gcccccaggc gtttacaata aactttccag ccagtcggta
     4861 aacgcccgta cttttcggga gagatggcgg tgcggataca gaagttacag tttgcgattc
     4921 ggggacggat tatctggcag aacctctacc aacagcccgc gatcgagata aggctgcaaa
     4981 aaaatattca tgccctgtag tattcccagc cccgccaggc ctgctgcaat gtaggcttct
     5041 gagttatcca cgacaagcct gcctggaagt tcaatttcgg tggagccctc tgacgtcccg
     5101 aatgtccagg gcataacttg tctgttgctg ctgttaatcc agttgacggc ctgatgctgc
     5161 tgcaactcgt ccattgtttc cggggtgccg tgttgatcaa gatacgcagg actggcgcag
     5221 gtcgtcattt taacggttcc caccttcttt gcgatatagt cgccatcgtc cagctcccct
     5281 accctcagta tgcagtccag gccatcatgc agcatattcc gtcggcggtc tgagctgctc
     5341 aggataattt caattccggg atgctggcga taaaaatcag gcaggttggg tataacaagt
     5401 aaagccgcaa acgcgacagg catatctatg agcgaatagc ggacgtcaaa atgatagcga
     5461 caggaaagtt ctgccatatt aaacataaca acccttgggc gcgccgccgc aggcgccctc
     5521 agcacgtttc tgcggcctcc cgcctgtttc cggcaccttt cctgccccaa cccctgaaaa
     5581 acgcccctga tgatttctgc gcgtctgcat ggctatgcag tgagatctgc tctccaccga
     5641 ttagtgcatg cagattttgg aaacgtccaa ttctggcacg aagcggtcac cccggcgggg
     5701 tgcagtccgc tgtcagcgaa aacggacatt gagccaatca aataatctgt gccagaattt
     5761 gtcattgagg ctagtacttt cgagtagatc tatttcccgc ccgtactctt aaactcatct
     5821 atctcatcct tctacgactt attaccggga atataaaacc ccgccgaagc gggtttggta
     5881 atttgagttt ttctcaggcg gccatctttc tgcattgttc ggcgcagtga tgacaagcgc
     5941 ttgagcaatc ctgacaatgg tcatgttcat gctttccaca ctcgtcaccg cacttctgac
     6001 aaacatcagc gcaaagacgg caaagatcct gggcagaccc gctatttagt gtcatgaatt
     6061 gagctgcaag acggcagata tttgcacact gcatatccag ttttatgcac tcgcgcatca
     6121 tctccagatt ctcttcgtcg agacacgaag ccgcacagtt atcacatgcc gttgcacata
     6181 gataacaggc ttcgatgcat tttttgtatt cgtctaacat aatttctcct tagctaaggt
     6241 tacgtattaa gcatggaaga gataactgat tattcaaatt ttgaattagt taataacctt
     6301 ttcagcattt catatcattg attcaaagtg catctctagg gttacctaat tcacctatgt
     6361 ctacttctgg cacaatgcag aaatgagata gatttacttt atccgattct cgctcaaagc
     6421 tgtccgtaat cccctgcctg cttaaaaaaa ccggattaac gtgtgatgtt tttacgtttt
     6481 ccaggcagcc ttcatcggta agtacctgtc ttacagactt caatgcactt agttaagaaa
     6541 catcctaatc gtcctttcgc cgatcgcgag atctgttatg gcagctaaac tcgattgata
     6601 ccccaatccg ccgggcagca tttcctttcc gcacgcaaga aggagaagat catgaaagca
     6661 gtaatttaca acggtcctta tgatgtaaaa gtaaaagacg taccggatgc gaaaattgtc
     6721 cgccccacgg atgtactcgt ccgcatcacc accaccaaca tttgtgggtc ggacttgcac
     6781 atgtatgagg gccggaccag ctttgaacag ggacggattc tgggtcacga aaacctgggt
     6841 gaagtggtcg aaattggctc aggcgttgaa cgtattaagg tgggcgacta tgtttgtctg
     6901 ccgtttaacg tgggatgtgg tttttgcgaa aactgtgaaa aaggtctgac cggcttttgt
     6961 ctcactacca atccgggttc ggcaggcgca gcttatggtt ttgctgaaat gggtccctgg
     7021 gagggggggc aggcggaact gcttcgggtg ccatttgccg actttaactg ccttgttctt
     7081 ccccctgatg cggcagaaaa agaagatgat tatgtgatgc tctctgacat ttttcctacc
     7141 ggctggcatg caacagaact tgccggattg aagcctggtg aaagcgtggc catttatggc
     7201 gcggggccgg tcggtttgat ggcagctcat tcggcgatca ttaaaggtgc ttcacaggtg
     7261 tttgtggttg atacgcacac agaccggttg gctctggctg aaaaaatggg ggcgacgggc
     7321 atcaatgcta ctggcgatga agctgtacaa aaaattctcg acctgacgga tggacgcggt
     7381 acggattgtg gctgcgaatg tgttggctat cagtgctgta acaaacatgg ccatgaagat
     7441 aactctgtca ccatgaacag tcttgttgcg tcgacgaaag caaccggggg aattggtgtg
     7501 gttggcgtct ttattcctca ggatccgggg gctgaatcgg agcttgccag agagggtaag
     7561 atgccatttg attttgggag tttctggttt aaaggacagg caatccgtac cggccaggcg
     7621 aacgttaagg cctataaccg tcagctgtct cgcctcatcc atcacgataa agccagtccg
     7681 ggcaaaataa tttctcatcg tctttcgctt gaagaggctc ctgccggata taagcacttc
     7741 gatgagcggg atgaaggatg gacgaaggtt atccttaagc cttaaataga gttatccggt
     7801 atgagaaagc aaaaaccggg aaagaatgta tgacttttga cagggtgcaa taaaatatcc
     7861 tgtcagggcg ttattattca tactttcagc tgccaggtga tgctgccaac ttactgattt
     7921 agtgtatgat ggtgtttttg aggtgctcca gtggcttctg tttctatcag ctgtccctcc
     7981 tgttcagcta ctgacgggg
//
LOCUS       DAPHBJ010000199         7933 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.201, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000199 DAPHBJ010000000
VERSION     DAPHBJ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.201"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            53..148
                     /locus_tag="RHZ76_003830"
     CDS             53..148
                     /locus_tag="RHZ76_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814385.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            312..2987
                     /gene="acnA"
                     /locus_tag="RHZ76_003831"
     CDS             312..2987
                     /gene="acnA"
                     /locus_tag="RHZ76_003831"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HDV2814386.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKGIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(3051..3641)
                     /gene="ribA"
                     /locus_tag="RHZ76_003832"
     CDS             complement(3051..3641)
                     /gene="ribA"
                     /locus_tag="RHZ76_003832"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HDV2814387.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            3811..4575
                     /gene="pgpB"
                     /locus_tag="RHZ76_003833"
     CDS             3811..4575
                     /gene="pgpB"
                     /locus_tag="RHZ76_003833"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HDV2814388.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKGKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            4724..5032
                     /gene="lapA"
                     /locus_tag="RHZ76_003834"
     CDS             4724..5032
                     /gene="lapA"
                     /locus_tag="RHZ76_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HDV2814389.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            5039..6208
                     /gene="lapB"
                     /locus_tag="RHZ76_003835"
     CDS             5039..6208
                     /gene="lapB"
                     /locus_tag="RHZ76_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HDV2814390.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            6401..7138
                     /gene="pyrF"
                     /locus_tag="RHZ76_003836"
     CDS             6401..7138
                     /gene="pyrF"
                     /locus_tag="RHZ76_003836"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDV2814391.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            7138..7464
                     /gene="yciH"
                     /locus_tag="RHZ76_003837"
     CDS             7138..7464
                     /gene="yciH"
                     /locus_tag="RHZ76_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HDV2814392.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(7590..7808)
                     /gene="osmB"
                     /locus_tag="RHZ76_003838"
     CDS             complement(7590..7808)
                     /gene="osmB"
                     /locus_tag="RHZ76_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HDV2814393.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
BASE COUNT         1936 a         1882 c         2124 g         1991 t
ORIGIN      
        1 taacagcttg agttatctca acacaaaata ataaccgtta agggtgtagc ctatgatcaa
       61 cacaaatatg aaatattggt cctggatggg cgcgttttct ctgtcgatgc tcttctgggc
      121 cgaactcctc tggatcatta ctcactgatc cttgaccccg ctgcggcggg gttgtcattt
      181 gctttgccac aaggtttctc ctcttttatc aatttgggtt gttatcaaat cgttacgcga
      241 tgtttgtgtt atctttaata ttcaccctga agagaatcag ggcttcgcaa ccctgtcatt
      301 aaggaggagc tatgtcgtca accctacgag aagccagtaa ggacacgttg caggccaaag
      361 ataaaactta ccactactac agcctgccgc ttgctgctaa atcactgggc gatatcaccc
      421 gtctacccaa gtcactcaaa gttttgctcg aaaacttgct gcgctggcag gatggtaact
      481 cggttaccga agaggatatc cacgcgctgg caggatggct gaaaaatgcc catgctgacc
      541 gtgaaattgc ctaccgcccg gcaagggtgc tgatgcagga ctttaccggc gtacctgccg
      601 ttgttgatct ggcggcaatg cgcgaagcgg ttaaacgcct cggcggcgat actgcaaagg
      661 ttaacccgct ctcaccggtc gacctggtca ttgaccactc ggtgaccgtc gatcgttttg
      721 gtgatgatga ggcatttgaa gaaaacgtac gcctggaaat ggagcgcaac cacgaacgtt
      781 atgtgttcct gaaatgggga aagcaagcgt tcagtcggtt tagcgtcgtg ccgccaggca
      841 caggcatttg ccatcaggtt aacctcgaat atctcggcaa agcagtgtgg agtgaattgc
      901 aggacggtga atggattgct tatccggata cactcgttgg tactgactcg cacaccacca
      961 tgatcaacgg ccttggcgtg ctggggtggg gcgttggtgg gatcgaagca gaagccgcaa
     1021 tgttaggcca gccggtttcc atgcttatcc cggatgtagt gggcttcaaa cttaccggaa
     1081 aattacgtga aggtattacc gccacagacc tggttctcac tgttacccaa atgctgcgca
     1141 aacatggcgt ggtggggaaa ttcgtcgaat tttatggtga tggtctggat tcactaccgt
     1201 tggcggatcg cgccaccatt gccaatatgt cgccagaata tggtgccacc tgtggcttct
     1261 tcccaatcga tgctgtaacc ctcgattaca tgcgtttaag cgggcgcagc gaagatcagg
     1321 tcgagttggt cgaaaaatat gccaaagcgc agggcatgtg gcgtaacccg ggcgatgaac
     1381 caatttttac cagtacgtta gaactggata tgaatgacgt tgaagcgagc ctggcagggc
     1441 ctaaacgccc acaggatcgc gttgcactgc ccgatgtacc aaaagcattt gccgccagta
     1501 acgaactgga agtgaatgcc acgcataaag atcgccagcc ggtcgattat gttatgaacg
     1561 gacatcagta tcagttacct gatggcgctg tggtcattgc tgcgataacc tcgtgcacca
     1621 acacctctaa cccaagtgtg ctgatggccg caggcttgct ggcgaaaaaa gccgtaactc
     1681 tgggcctcaa gcggcaacca tgggtcaaag cgtcgctggc accgggttcg aaagtcgttt
     1741 ctgattatct ggcaaaagcg aaactgacac cgtatctcga cgaactgggg tttaaccttg
     1801 tgggatacgg ttgtaccacc tgtattggta actctgggcc gctgcccgat cctatcgaaa
     1861 cggcaatcaa aaaaggcgat ttaaccgtcg gtgcggtgct gtccggcaac cgtaactttg
     1921 aaggccgtat ccatccgctg gttaaaacta actggctggc ctcgccgccg ctggtggttg
     1981 cctatgcgct ggcgggaaat atgaatatca acctggcttc tgagcctatc ggccatgatc
     2041 gcaaaggcga tccggtttat ctgaaagata tctggccatc ggcacaagaa attgcccgtg
     2101 cggtagaaca agtctccaca gaaatgttcc gcaaagagta cgcagaagtt tttgaaggca
     2161 cagcagagtg gaagggaatt aacgtcacac gatccgatac ctacggttgg caggaggact
     2221 caacctatat tcgcttatcg cctttctttg atgaaatgca ggcaacacca gcaccagtgg
     2281 aagatattca cggtgcgcgg atcctcgcaa tgctggggga ttcagtcacc actgaccata
     2341 tctctccggc gggcagtatt aagcccgaca gcccagcggg tcgatatcta caaggtcggg
     2401 gtgttgagcg aaaagacttt aactcctacg gttcgcggcg tggtaaccat gaagtgatga
     2461 tgcgcggcac cttcgccaat attcgcatcc gtaatgaaat ggtgcctggc gttgaagggg
     2521 ggatgacgcg gcatttacct gacagcgacg tagtctctat ttatgatgct gcgatgcgct
     2581 ataagcagga gcaaacgccg ctggcggtga ttgccgggaa agagtatgga tcaggctcca
     2641 gtcgtgactg ggcggcaaaa ggtccgcgtc tgcttggtat tcgtgtggtg attgccgaat
     2701 cgtttgaacg aattcaccgt tcgaatttaa ttggcatggg catcctgccg ctggaatttc
     2761 cgcaaggcgt aacgcgtaaa acgttagggc taaccgggga agagaagatt gatattggcg
     2821 atctgcaaaa cctacaaccc ggcgcgacgg ttccggtgac gcttacgcgc gcggatggta
     2881 gccaggaagt cgtaccctgc cgttgtcgta tcgacaccgc gacggagttg acctactacc
     2941 agaacgacgg cattttgcat tatgtcattc gtaatatgtt gaagtaacaa ctatttgctt
     3001 gccggttatt ttgcttccgg caagcaaatg aattacacaa tgcaagaggg ttatttgttc
     3061 agcaaatggc ccattttctc ggctttggta tcgagataat gttcgttatt ggggttacga
     3121 cctacaatca atggtacgcg ttcaacaata ttaatccctg cttcggtcag aatttcgact
     3181 tttttcgggt tattggttaa caagcggact tcattgacgc caaggagttt gaacatatca
     3241 gcgcaaagag tgaagtcgcg ctcatcagcg gcgaagccta actggtggtt agcctctacg
     3301 gtatcgtaac cttgatcctg cagtgcgtaa gcgcggattt tattcagcag accaatgtta
     3361 cgaccttcct gacggtgata cagcaaaata ccacggcctt cctcggcaat ttgcgtcaat
     3421 gccgcttcga gctggaagcc acaatcgcag cgcaagctga acagggcgtc accggtcaga
     3481 cattcggaat ggacgcgcgc aagtaccggg gtatgcccgg aaatatcgcc atagactagc
     3541 gcgacatgat cgtgtccggt tgccagttct tcaaatccca ccatcaggaa atcgccccat
     3601 ggggttggca gtttggcttc tgccacacgt ttaagctgca tgaaattctc cagataatgc
     3661 tggttctgta ttggcttatt ttgccataac gagaaggggt tcacctaatc acgacgcgtc
     3721 gatcgttcac ggaatggcac aaatctgtca atttttcctg gaactggcgt tttcagttat
     3781 gattgtggga cttatcaaaa aggagaggcc atgcgttcga ttgccagacg taccgcagtg
     3841 ggagctgcac tattgcttgt catgccagta gccgtatgga tttctggctg gcgttggcaa
     3901 cctggagaac aaagttggct actaaaagcg gctttttggg ttactgaaac tgtcacccag
     3961 ccctggggcg tcattacaca tttgatttta ttcggctggt ttctctggtg tctgcgtttt
     4021 cgcattaagg ctgcctttgt attatttgcc attctggcgg ccgcaatcct tgtgggacaa
     4081 ggcgttaaat cctggatcaa aggcaaagtc caggaaccac gaccttttgt tatctggctg
     4141 gaaaaaacac atcatattcc ggttgatgag ttctacactt taaagcgagc agaacgcgga
     4201 aatctagtga aagaacagtt ggctgaagag aaaaatatcc cacaatattt gcgttcacac
     4261 tggcagaaag agacggggtt tgcctttcct tccggtcaca cgatgtttgc tgccagttgg
     4321 gcactgctgg ccgttggttt gctgtggccg cgtcggcgaa cgttaaccat tgctatcttg
     4381 ctggtctggg caacgggagt catgggaagc cgcctgctgc tcgggatgca ttggccacgc
     4441 gatctggtag tagctacgtt gatttcgtgg gcgctggtgg cggtggcaac gtggcttgcg
     4501 caacgaattt gtgggccatt aacaccacct gcggaagaaa atcgcgaaat agcgcaacga
     4561 gaacaagaaa gttaacgctg gttgattttc cgaatttagc ccttaaatca tcaacaatgc
     4621 gtgtggatgc catttcgcag acggcgcgaa aatggtactt taaagggcta ttgcggtaag
     4681 ttgaccataa tttattcgct ctaaccacat aacgggaagt aatgtgaaat atttactcat
     4741 tttcttactg gtgttagcga tcttcgtgat ttcggtcacg ttgggtgcgc agaacgatca
     4801 acaggtgacg tttaattatc tgttagcgca aggggagtac cgtatttcca cattgctggc
     4861 ggtattgttt gctgcggggt ttgctatcgg ttggttgatt tgtggcctgt tctggctgcg
     4921 agttcgtgtt tccctggcgc gcgctgaacg taaaataaag cgactggaaa accagctttc
     4981 acccgcgact gacgtggctg tagtgccgca ctcgtcagcg gcgaaggaat aactttctat
     5041 gctggagttg ttgtttctgc ttttgcctgt agccgctgcc tatggctggt atatgggccg
     5101 cagaagtgcg caacaaaaca agcaagatga agccaaccgc ttgtcgcgtg attacgtagc
     5161 gggggttaac ttcctgctta gtaatcaaca ggataaagcg gtagacctgt ttctcgatat
     5221 gcttaaagag gatacaggca ccgttgaagc ccaccttacg ctcggaaacc tgttccgttc
     5281 gcgtggcgaa gttgatcgcg ctattcgcat ccatcagacc ctaatggaaa gcgcctcgct
     5341 gacctatgaa cagcgtctgt tggcgattca acaactgggg cgtgattaca tggccgccgg
     5401 gttatatgac cgcgcggaag acatgttcaa tcagctgacc gatgaaactg acttccgcat
     5461 tggcgcgctg caacagttgc tacaaatcta ccaggctacc agtgagtggc agaaagcaat
     5521 tgatgttgcc gaacgcctgg tgaagctggg taaagataaa cagcgcgtcg aaattgccca
     5581 tttctactgt gagttagccc tgcagcatat ggccagcgac gatctcgatc gtgccatgac
     5641 cttgctaaaa aaaggggcgg cggcagataa aaacagcgcc cgcgtatcca taatgatggg
     5701 acgcgtgttt atggcgaaag gagaatacgc caaagccgtc gaaagtctgc aacgcgtgat
     5761 atcccaggac agagaactgg tcagcgaaac gctggaaatg ttgcaaacct gctatcagca
     5821 gttgggtaaa actgccgaat gggcagaatt cctgcagcgt gcggtggaag agaacaccgg
     5881 tgccgatgct gaattgatgc ttgctgatat catcgaagcg cgcgacggta gtgaggccgc
     5941 acaggtctat attacgcgcc agcttcagcg tcatccgacc atgcgtgtgt tccataagtt
     6001 aatggattac cacttaaatg aagcggaaga agggcgtgcc aaagagagcc tgatggtgct
     6061 gcgtgacatg gttggcgaaa aggtacgtag taagcctcgt tatcgctgcc agaaatgtgg
     6121 ttttaccgca tacaccctct actggcattg tccgtcttgt cgggcctggt caaccattaa
     6181 accgattcgc ggtcttgatg gcctgtaatt tttaaaaaaa tcctacttta gttacaacat
     6241 actagttatt gatgttccat tgtgctccgg caacgacggc gcagaaaaag cctgccaggg
     6301 gaaaaatcgc aactgttaat tttttatttc cacgggtaga atgctcgccg tttacctgtt
     6361 tcgcgccact tccggtgccc atcatcaaga aggtctggtc atgacgttaa ctgcttcatc
     6421 ttcttcccgc gctgttacga attctcctgt ggttgttgcc cttgattatc ataatcgtga
     6481 tgacgcgctg tcctttgtcg acaagatcga cccacgcgat tgtcgtctga aggtcggcaa
     6541 agagatgttt acattgtttg ggccacagtt tgtgcgcgaa cttcaacagc gtggttttga
     6601 tatctttctt gacctgaaat tccacgatat tcctaacact gcagcgcacg ctgtcgctgc
     6661 tgcagctgac ttaggcgtgt ggatggtgaa tgttcatgcc tctggtgggg cgcgtatgat
     6721 gaccgcagcg cgtgaggcac tggttccgtt tggcaaagat gcaccgcttt tgattgctgt
     6781 gacagtgttg accagcatgg aagccagcga cctggtcgat cttggcatga cactgtcacc
     6841 tgcagattat gcagaacgtc tggcggcact gacgcaaaaa tgtggccttg atggtgtggt
     6901 gtgttctgct caggaagctg tgcgctttaa acaggtattc ggtcaggagt tcaaactggt
     6961 tacgccgggc attcgtccgc aggggagtga agctggtgac cagcgccgca ttatgacgcc
     7021 agaacaggcg ttgtcggctg gtgttgatta tatggtgatt ggtcgcccgg taacgcaatc
     7081 ggtagatcca gcgcagacgc tgaaagcgat caacgcctct ttacagcgga gtgcatgatg
     7141 agtgattcca acagccgtct ggtctactca acggaaaccg gacgtattga tgagcccaaa
     7201 gcggcccctg tacgccctaa aggcgacggt gtggtgcgta ttcagcgtca gaccagtgga
     7261 cgtaaaggta agggcgtttg cctgattacc ggtgtcgatc tcgatgatgc cgaactgaca
     7321 aaacttgcag cggaactgaa gaaaaaatgc ggctgcggcg gagcagttaa agatggagtt
     7381 attgaaatcc agggcgataa gcgtgattta ttaaaatcac tacttgaagc gaaagggatg
     7441 aaggtaaaac tcgcaggcgg ttaacataaa aagccacgga tatatccgtg gctttcgaat
     7501 attttactgt gtgtattatt cagttatttc gtactgaaca gaatcaagcg tattataatt
     7561 gcgagtcgat aatcgacatt tatttacgat tatttaccga cctggtgacc aataacacca
     7621 ccgacagctg caccacctaa tgtacccaac gtactgccat cggtcagtac tgcaccgcct
     7681 aatgcccctg cacccgcgcc gattgcggtg ttgcggtccc gtttagacca gttagaacag
     7741 gcactcagag acattgccaa agtaattgcc agaacagccg cggtcatttt tttgctcgtt
     7801 acaaacataa tactctctcc tgaatttatg attcacggaa gtaagctctc tataactata
     7861 atagctaaga ataagcttgg tgaaattatt ccgtgaaatc tgctcgcgcg cagggttata
     7921 tcacgcaggt gat
//
LOCUS       DAPHBJ010000200         7858 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.202, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000200 DAPHBJ010000000
VERSION     DAPHBJ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.202"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(34..879)
                     /locus_tag="RHZ76_003839"
     CDS             complement(34..879)
                     /locus_tag="RHZ76_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDV2814394.1"
                     /translation="MSAITVSRPEVVNGHTDVICSTSVSHILAVRKTTLLQIDTLIRQ
                     LAEISSMTESIGGKTSLDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANIWSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRGDRWGLHLITGQQADRLADLERMLYLFSGKPIPDNR
                     ENIIIRLDDHIRSVQGKACYEDEMFNIRYFKKGSAHITFRKPELVDRLNDIIARHYSE
                     MLPSL"
     gene            complement(964..1161)
                     /locus_tag="RHZ76_003840"
     CDS             complement(964..1161)
                     /locus_tag="RHZ76_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HDV2814395.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAWEDIRTLRQLQLPDQNR"
     gene            complement(1181..1669)
                     /locus_tag="RHZ76_003841"
     CDS             complement(1181..1669)
                     /locus_tag="RHZ76_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814396.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLSELINKVSENGYS
                     LRVVDKSDQHATSTPPPLTTLTCIRCSTAHITETDNAWLYSLSHQTNDDGETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDAGAECLPGLPTF
                     DW"
     gene            complement(1666..2043)
                     /locus_tag="RHZ76_003842"
     CDS             complement(1666..2043)
                     /locus_tag="RHZ76_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDV2814397.1"
                     /translation="MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRNNYRMVNNITQGKHPEAKR"
     gene            complement(2090..2467)
                     /locus_tag="RHZ76_003843"
     CDS             complement(2090..2467)
                     /locus_tag="RHZ76_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDV2814398.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTITLYAKGLTCEAD
                     TLGSCGYAYLAVYPTPAEPAITV"
     gene            complement(2517..2663)
                     /locus_tag="RHZ76_003844"
     CDS             complement(2517..2663)
                     /locus_tag="RHZ76_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814399.1"
                     /translation="MKAKQENRLERQLQHPENEYDWLSSDAAVDEVLLINEYTFTEAG
                     LRAG"
     gene            complement(3303..3905)
                     /locus_tag="RHZ76_003845"
     CDS             complement(3303..3905)
                     /locus_tag="RHZ76_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814400.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLKHHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWTENLKRK
                     SEKELEEWIIDRQKQSAALTDLMDQYVLLAYRTTVDDNRN"
     gene            complement(4000..4206)
                     /locus_tag="RHZ76_003846"
     CDS             complement(4000..4206)
                     /locus_tag="RHZ76_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDV2814401.1"
                     /translation="MTTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            4914..5126
                     /gene="hha"
                     /locus_tag="RHZ76_003847"
     CDS             4914..5126
                     /gene="hha"
                     /locus_tag="RHZ76_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HDV2814402.1"
                     /translation="MEVKTKEDWLYQFRRCSSRETLEKVISHTRYKLTPAELETFNSA
                     VDHRLAELTMNKLYDRVPASVWKYVI"
     gene            5870..6439
                     /locus_tag="RHZ76_003848"
     CDS             5870..6439
                     /locus_tag="RHZ76_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDV2814403.1"
                     /translation="MTHEYNPFWQQRIRETVLRALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARINAYQKRKRREGKRVHPTTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPRSLAGMIKQAWCSALGVDAGPYATLAHFPDTSAVWLERDDDTGFQ
                     QVLERADYLAKEHTKAHATGERNFGCSRS"
     gene            6605..6988
                     /locus_tag="RHZ76_003849"
     CDS             6605..6988
                     /locus_tag="RHZ76_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814404.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTAHGQQCPYVRPERREVRLIQRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGRFSEEVVAG"
     gene            6985..7410
                     /locus_tag="RHZ76_003850"
     CDS             6985..7410
                     /locus_tag="RHZ76_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814405.1"
                     /translation="MNGIKMGLGITPGEHIISANSALSRNIRHCFCLSCRGRLILQTD
                     AQGAWFEHDLHALSAQQKAACVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACATGIYCRAVCTRSVYSYQPDLFEDCGGAST"
     gene            7656..7852
                     /locus_tag="RHZ76_003851"
                     /pseudo
     CDS             7656..7852
                     /locus_tag="RHZ76_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305349.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
BASE COUNT         1820 a         1963 c         2128 g         1947 t
ORIGIN      
        1 ctaaaataca taattgaatt cattcttaga gctttataat gaaggtaaca tttccgaata
       61 gtgcctggca ataatatcat ttagccgatc aaccagttct ggtttcctga acgtgatgtg
      121 tgcggagccc ttcttaaagt atctgatgtt gaacatctca tcttcatagc acgctttgcc
      181 ctgaacggag cggatgtgat catccagccg aatgatgata ttttcccggt tgtcagggat
      241 tggtttgcca ctgaacagat acagcattct ttccaggtcg gcgagtcgat ctgcctgttg
      301 cccggtgata agatgcaatc cccaccggtc ccccctcacc agattgttga cgataatttt
      361 actgccaaat ttacaggggg agttggtttt gtaattccag ctcagccctc tgaagacgtt
      421 gatcactcct cgctcaaaca cttcatcctt attctggtgc agttgctcaa atgtgctcca
      481 aatgttcgct tcactgattt ccggaaagtt atcgtactcc agtgagcggt accatgtatc
      541 acgggcctgc gcatccatta atgacagcat cccggaacgt tgcatcaggt cacgccagat
      601 ttcgcggtca agattgcggg tgatggcttt cattgcggtt tccggtttct ccatgagcca
      661 gcagccacag cgaaaatcct gtttcatggc ccagtccaga gaagttttac ctccaatact
      721 ttctgtcatt gatgagattt cagccagttg ccggataagt gtgtcgattt gcagcagtgt
      781 agtctttcgc acagccagaa tgtggctgac tgaggtggag cagataacgt ccgtatgccc
      841 gttgaccact tccggacggg agactgtgat tgcagacata atcgatattc cttttcgcag
      901 ataaatgaaa acgccagccc tgttcaggct ggcgtcagtg gaatgaagta tggtgaatga
      961 gactcaccgg ttctggtctg gaagctgcaa ctggcgcagg gtacggatat cttcccaggc
     1021 ctgttcaata cagggcagca gtgctgccga atccgtttta tcctcatcgt tgtcgaagat
     1081 aatccctgat tcgcagtcaa tattgtcctg cagccacgca atcagaatat ccagcgccgt
     1141 ttctgtggtt aataatttca tccagtgtct tttgtcctga ttaccagtcg aaagtgggga
     1201 gccccggcag acattctgcg ccggcgtcca gatgaatgag actgacgcca taacgccggg
     1261 tgagagtgac gaccagacga cggaacgttc tggacaggcc caggcgttta agccgcagaa
     1321 ccgggtacga ccatgcatcg gtacgtaaca gatagccgct gcctgtaaaa tgaatccatt
     1381 ctgtttcgcc gtcgtcatta gtctggtgtg acagcgagta cagccaggcg ttgtccgttt
     1441 ccgtgatatg tgcggtgctg caacgtatgc aggtaagggt tgttaacggt ggtggtgtac
     1501 tggttgcgtg ttggtcggat ttatcgacca cgcggagtga ataaccgttt tcagagacct
     1561 tgttaatcag ttcactgaga ttaacaccat cgacgtcaac gacaatgcgc cccatgttca
     1621 gtgcctgcac gttaacgctg tcggcttcca gcgtcagggc cagtttcatc gttttgcctc
     1681 cggatgcttg ccctgggtaa tgttatttac cattctgtaa ttgttccggg tcatcaggcc
     1741 ggtcgccctg cgagcccgga gaatatcaat gctgttgatt aactgcgacg gggctcctgc
     1801 gctgaatccc ggctggtcgg tacgtaccag cgcgtatttt tcaacgagaa agttcaccgc
     1861 gtcacacagt gaaatccctg cctcaatatg ctgctcaatc acacgttcat cagcgaacgg
     1921 tgtgtcattc agcgtgaggc catagtgctg gtccagcagt cgggtgagca gtgtctgcca
     1981 gatggtgacg ggagacgggc agcccgatgc ctcccgtacg tgagtgtcgg gtaatgtatt
     2041 catcttaagt tctctgtaac aggtggtgat aaaggggaga tatccggggt tatacggtga
     2101 ttgcgggctc tgccggtgtc ggataaacag ccagataagc gtaaccacaa ctgccaaggg
     2161 tatcggcttc gcaggtcagc cccttcgcat acagtgtgat ggtatgctga tggcggggat
     2221 tcagttcacc gctggtgagc atgagttcca gttgtttcat cagcagcgga aaggcctggt
     2281 ccaggtggta tgcatccgcg tcgctgaacc ggcctctgat accggcacgg tcggcaaggt
     2341 aatgcaaccg gttaccctcc tgcaccagac gggcgccgaa acagggcgtc actgtgcagg
     2401 gtagtcccca ccagggatgg tcgtcattgt catcgggatg cgttgtcccg gaaagtgtgt
     2461 ctgacacgat aaaatcctca cagaaaatcg gtgaatgata gtttaacgat gacgggtcag
     2521 ccggcgcgca gaccggcttc ggtaaaggtg tattcgttga tgagcaggac ctcatccaca
     2581 gcggcgtctg agctcagcca gtcgtactcg ttttccgggt gttggagctg tcgctcaagc
     2641 cggttttcct gtttcgcttt cattagcacc attttgtgac gggtccttat ggctgtcggt
     2701 tcagggtatg tttcctggtt attgtgtacc acgaagcagg ggtattgtgt cgatggtcac
     2761 atttcagcac cataatgccc agaatgctcc ctgattccac agatcactca actcaccgtc
     2821 cgttcctaca accggcaata cccgtaacct gtgccacaca gacggagccg ggccatgtgc
     2881 agagaccggt aaaattcgtt actacaactg ttcctgaata tctgacgaag aaacaatgat
     2941 ctccggggac ttcagccata acatcctgcc gtgtgagttt cttccgcagt gcaaggagcg
     3001 ctgtcgccat gtacttattg tcaccaggat gaggcatgta ccgcagcatg ccatttctgg
     3061 taaataacct ctgcctgcat attcagtccg tagagaacgt ggcgtgtatc tggctgtaaa
     3121 gaacatccct gtccatggct ttcattcact ggtggtgttc atgccagttc agcaatgaca
     3181 ggatgccctt aagccatccg tcatggctca gtatgatgac gacgaacacg ctctgcaggt
     3241 gagtatgtgg ggccggaagc atcattcccg tattttgccg caccttccgg aagcaaccgg
     3301 catcagttac ggttatcgtc aaccgttgtg cggtatgcca ggagcacgta ctgatccatc
     3361 agatccgtca gtgctgcgct ctgcttctga cggtcgatga tccattcctc cagctctttt
     3421 tctgatttcc gtttcaggtt ctctgtccag tcttcaaggc tgtttccggt cattaaatca
     3481 agccagcaaa gccagaccag tttaaggcgt aactccgtgc caccaaactg acggaagaga
     3541 ctgatcagtt tatttgccag agaatggtgt ttcagacgtt cacattcaga cagcagaatg
     3601 ccttccagca tcggatgatg aaccagcaga tgttcactgg tgcataaatt cactatcaga
     3661 tcatcatcca ggggcggcgt ggcccgtttg tcagggccat aaatcagccg gtgcaggctc
     3721 tgggggacga tggcaatctc cggtttttca ccattgaatg acacccaacc gggcgtcaga
     3781 ttatggaagt agtagatatt cggatgacac acgccccagg atacggcgtg ccgggtgaag
     3841 ccggtcagcc atttctggta gctttcgtgg agccattcgt agcgggtctg atactgttgt
     3901 ttcattttca tattcctcac ggggatataa caggcagcag aatgccgtcg gcaggccggg
     3961 gattacgtct gctgcgggtg aacggataag gggcagaaat tacggacggg actgtgcaat
     4021 acgcgcctgt agccaggctt ccacttcact tttcagccag cgggagctgc ggccgagttt
     4081 gatgggagcc ggaaaggccc catccttgat gagcttgtaa aaccacttat cggtcaggcc
     4141 ggtcagctga gtgataaacg ccatatcgac catctggtca tccatcagcg aaactggggt
     4201 ggtcatgaga atgttcctcc ggatgttgtt aaaccagccg gtgtggtcat gaaaacaacg
     4261 caccaccttc accggcataa caaccgggga gaaacgtaaa aaataatcgg gaatgtgacc
     4321 gccaggagat aaacggggag gttgcggtgg ctgagccgta ccggtatgcc gggtggttgt
     4381 tacgaaacga tgcttccgca cgcgcgcgcg ataaagaagg gatgattttc gtgaggaaga
     4441 ggggaaaact ggcgttctga taattgcaga gttatttacg gggcggttac ggtggggcag
     4501 cccgtgcctt gtgggcaggg acaggtatca ctgttgggat aagactgtct gattggtatt
     4561 ataaatatat aagagagatc aaacacatga gttcatatat gtgaaataaa cactgtgagt
     4621 gcgatcagtg ggcatgagaa cgcagtagca gcagattgcg ttgtacccac agggctatct
     4681 ctgatagctg gcgtataacc tgcgtatata aaacgtacct gcaacccacg tgaacagagt
     4741 gcattaagca tccgtctgag cggtaagcgt atccgacctg gtgagtgaca gtctgagaga
     4801 ccggttaagt ggtggcccgg gagtaatgag tttggggagg gtaccagaat gccagtctgg
     4861 tgcggtatgc tgacagtatc acgttaattg tagcataaag tgatgagagg ctaatggaag
     4921 ttaaaaccaa agaggactgg ctgtatcagt tccgtcgttg ttcatcccgg gagacactgg
     4981 aaaaagtgat ttcccacacg cgttataaac ttacccctgc ggagctggaa accttcaact
     5041 ctgcggtcga tcaccgactg gcagaactga caatgaacaa gctttacgat cgtgttcccg
     5101 cttcagtctg gaaatatgtc atctgagcgt tcggtaatca caacggcatc gcgccaggtg
     5161 ttatccgctt tgtgattctt attggctctt ctcaggttat taaaagctga atatgttcct
     5221 gttgtcggga accaggataa tgtcgggaac gttaagctgt ataacgacaa caaagaattc
     5281 gaaatcaaaa gggaatggtg tccggaggtg tgggtgatca tgggctgatt ttcgggtatt
     5341 aacggcctgt atctgctgag gttttctcgt atttaccggg gatccggtgc gttactgtct
     5401 ggcacagcaa acgcagaggc ggcggggcgt tctgccccgt tgattccgga taatccctcc
     5461 cctgtaacct actgcaacag tacaaataca ctgacgcctg aaacgacagc cagaatcagc
     5521 acaggcagta acgtcagtag aaagagcaga cgatcggtat ttcggtaata gcctgaggaa
     5581 gctgttgacg cgcagctcgg gcagcattcc gtattatgag taattgcgtt actacaatcc
     5641 ctgcacaccg ttttgcgtat caccataacg ccctcctcac agttttaccc gtaaccggtt
     5701 ttttgcatta aaaaaacaac gctgaaaatt caaccgtcag tccggagacg acagtcgggg
     5761 tatcactgag tgcctgagac attgcccggt tggagatcac ttcagggact ctgcacattt
     5821 tttacagcgt ggttattccg tatgaacgaa accatacgga gatttaacca tgacacacga
     5881 gtacaacccc ttctggcaac aacgtatccg tgagacggtg ctgcgcgcac tggatgttca
     5941 tccccgcctg acggcattgc gggttgacct gcgtctcccg gatgtaccgg cagcaacgga
     6001 cgcagctgtg atatcccgct tcatcaatgc cctgaaagcc cgaatcaacg cttaccagaa
     6061 gcgtaagcgc cgtgaaggta aacgcgtaca tcccacgacc ctgcattacg cctgggcccg
     6121 ggaattcggg gagcttaaag gcaaaaaaca ctatcacctc ctgctgctgg tcaaccggga
     6181 tacctggtgt cgtgccggcg attaccgcgc tccgagatca ctggccggga tgattaaaca
     6241 ggcctggtgc agtgccctgg gggtggatgc cgggccttat gccacgttgg cgcattttcc
     6301 tgacacgtcg gcggtgtggc tggaacgcga tgatgacact ggctttcagc aggtgctgga
     6361 acgtgctgac tatctggcga aggaacatac caaagctcac gccactggtg agcgcaactt
     6421 tggctgcagt cgtagctgag cccgacagac gagttatctg acctcagtgt gatgacgcgc
     6481 tcacggccac cacactgatt ttcccttctg agaccggact gaaaccgcca ttcacaccag
     6541 accaggctgc ccgtaccggg acggctgttg cctgcgtgtc gtctggcgct aaggaacata
     6601 tactatgtac gcaaaatctt ttctcgcact tgatggcaac ggacgtctga ccggtgcccg
     6661 taccgcacag actgcacctt atgatcgcta cacctgccac ctttgcggca gtgcactcag
     6721 ataccacccg caatacgaca ctgaacgtcc ctggtttgaa cacactgacg acgggctgac
     6781 agcgcacggt cagcagtgtc cttatgtcag accggaacgc agggaggtac gcttgattca
     6841 gcgtctgcag caattcgtac cggatgcctt acccgtggtg cgtaaagcca gctggcactg
     6901 cagacaatgt caccacgatt attatgggga gcggtactgc acacactgcc agaccgggcg
     6961 cttcagtgag gaggtggtag caggatgaac ggcataaaaa tggggctggg gatcacgccg
     7021 ggtgagcaca ttatcagtgc caactctgcg ctcagcagaa atatccggca ttgtttctgc
     7081 ctttcctgtc gtggccggtt gattttgcag acagatgctc aaggggcgtg gtttgagcat
     7141 gatttacatg ccctgagtgc gcaacagaag gcggcctgtg tggtgctgaa tccggagaag
     7201 tcccaccctt acattgagga tatggcgatg tttttatcac cattaccggt cgtgctggag
     7261 tggcattgtg tgatgtgtga gcagtttttt catgggaaga agttctgtga ggcctgtgct
     7321 acggggattt attgcagagc ggtatgtacg aggtctgttt acagctacca gccagacttg
     7381 tttgaggact gcggaggcgc gagtacttaa tccaaaatcg ccgctctgcg cagggcgttc
     7441 agaggtatcg caacctgcat tttatccggt gtttcgcacc tgccccgttc gtgacacaac
     7501 ggcgcgacac cggatatccg cagtcctgct tacctgaata aaacccgggg tattgattgg
     7561 tagtgtggtg cggaatcgta ctgtgatctc gcattcgccg aattcgcact gtggtgtcaa
     7621 tcaaccatac gtgagagtcc tctccgtatt tatcagtgcc tcaggacgat ttctgcatag
     7681 ttaatccagg ggctgcattg ttcatttccc tgctaatgga cgtgacatcc aggatattaa
     7741 cgatcctgat ttccctgagc agtatcagcg cttattggca cgtctggatt atctgacgaa
     7801 gcccggtact aaagcatcag gccagcgtaa ctctggctac agcggttttt gacctgtt
//
LOCUS       DAPHBJ010000201         7813 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.203, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000201 DAPHBJ010000000
VERSION     DAPHBJ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.203"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            376..1689
                     /locus_tag="RHZ76_003852"
     CDS             376..1689
                     /locus_tag="RHZ76_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HDV2814406.1"
                     /translation="MIKETVTMSHKELHRLQIIQEQAAARIGISIRQVKRLVQRYRNE
                     GPSGLVSHRRGKRPNNSFSTEFRATVISLLKGRYADFGPTFACEKLREIHGLSLSVET
                     LRKWMIEEGLWRERRRKIARIYQRRMRRPSYGELIQIDGSPHDWFENRGPRCTLIVFI
                     DDATSALMALRFVPAETTRAYMETLRGYLNDHGVPLALYSDRHSIFRVNNPEREGELT
                     QFTRAIKTLGIEPIHANSPQAKGRVERANQTLQDRLVKEMRLQNISDIETANAWLPTF
                     IEAYNNRFATSPRTTDNAHLDVHHSEEELGYIFSLQAKRVLSKNLTFQYKSSAFQVRS
                     EGRGYRLRHSVVTVCENFDGEINVLYDGKALGWEKYVDGPEPIPLDDEKSVHERVDNA
                     RIDLRSKYYVKPKADHPWLTRRTQSHQQVKPPKLPKKKPDPDKKD"
     gene            complement(2126..2458)
                     /locus_tag="RHZ76_003853"
     CDS             complement(2126..2458)
                     /locus_tag="RHZ76_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein FlxA"
                     /protein_id="HDV2814407.1"
                     /translation="MSVTIQGNTSTVISNNSAPEGTSEIAKITRQIQVLTEKLGKISS
                     EEGMTTQQKKEMAALVQKQIESLWAQLEQLLRQQAEKKNEDATVQPDKKEEKKDDTNT
                     AGTIDIYV"
     gene            complement(2661..2966)
                     /locus_tag="RHZ76_003854"
     CDS             complement(2661..2966)
                     /locus_tag="RHZ76_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814408.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLVFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMSVLLSRRRVSGDLTCR"
     gene            2991..3230
                     /gene="relB"
                     /locus_tag="RHZ76_003855"
     CDS             2991..3230
                     /gene="relB"
                     /locus_tag="RHZ76_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HDV2814409.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            3230..3517
                     /gene="relE"
                     /locus_tag="RHZ76_003856"
     CDS             3230..3517
                     /gene="relE"
                     /locus_tag="RHZ76_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HDV2814410.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            3589..3744
                     /gene="hokD"
                     /locus_tag="RHZ76_003857"
     CDS             3589..3744
                     /gene="hokD"
                     /locus_tag="RHZ76_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HDV2814411.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            3961..4212
                     /locus_tag="RHZ76_003858"
     CDS             3961..4212
                     /locus_tag="RHZ76_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814412.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDARVTDGFILREGEFVTSLQALKEIGIKAGFSAFSGE"
     gene            4279..4557
                     /locus_tag="RHZ76_003859"
     CDS             4279..4557
                     /locus_tag="RHZ76_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814413.1"
                     /translation="MARNAKYYNSDNSPVLACTHGRYSHAFKSEWFQHPPCTAEQAEW
                     LIHSYRRRGFEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
     gene            4559..5608
                     /locus_tag="RHZ76_003860"
     CDS             4559..5608
                     /locus_tag="RHZ76_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDV2814414.1"
                     /translation="MRVLLKPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHVTSCQWPNGDYHH
                     TETVIHRYGTGAMVLCWHCDNQLRDQTSESLELLAQQNLTAWVIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKICSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPYAHQQQKSPQEKTVVSITVDPESPESFMKLPKRRRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHTDTVAFEDKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            5622..6374
                     /locus_tag="RHZ76_003861"
     CDS             5622..6374
                     /locus_tag="RHZ76_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDV2814415.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGTGCLTITDVMAAQGMVQSK
                     APLGLALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVSFAFAD
                     YSRSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEASTA
                     CRGCKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIGKLVTKCWQEEAYAEAQLRKVTR"
     gene            complement(6652..6741)
                     /locus_tag="RHZ76_003862"
     CDS             complement(6652..6741)
                     /locus_tag="RHZ76_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814416.1"
                     /translation="MNNPVCLDDWLIGFKSLCCTLAVIALLII"
     gene            complement(6796..7008)
                     /gene="cspF"
                     /locus_tag="RHZ76_003863"
     CDS             complement(6796..7008)
                     /gene="cspF"
                     /locus_tag="RHZ76_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="HDV2814417.1"
                     /translation="MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEE
                     ITTGLRVEFCRINGLRGPSAANVYLS"
     gene            7309..7524
                     /gene="cspB"
                     /locus_tag="RHZ76_003864"
     CDS             7309..7524
                     /gene="cspB"
                     /locus_tag="RHZ76_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HDV2814418.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIESGAKGPAAANVIITD"
BASE COUNT         2120 a         1660 c         1948 g         2085 t
ORIGIN      
        1 gggtgttcat ggcggtcatg tgcattgaat actggcggta aatacagcga actccattgg
       61 tttagttgga tatttactgt gctggacaaa aacggtttgc ggggaaatct tagttaagta
      121 gaatgactgc gggtgcttga ggctatctgc ctcgggcatg gacaccaacg gcagatagag
      181 aaaagcccca gttaacatta cgcgtcctgc aagacgctta acattaatct gaggccaatt
      241 tcatgctaga cacatgtagg ttagcctctt acgtgccgaa aggcacggag aagcaggcta
      301 ttgttaacac caagctgtaa tgtccccttt gaaccattct aaaatgtccc cagacaattc
      361 tctgggggat ttttcatgat caaagagact gttacgatga gtcataagga actccaccga
      421 cttcagatta ttcaggaaca agctgcggca cgcattggca tttctattcg gcaggttaaa
      481 cgtctggtgc aacggtatag aaatgaaggg ccttctggtc tggtttccca ccgacgtgga
      541 aagcgtccta ataattcctt ttctactgaa ttcagagcaa cagtaatttc actcctcaaa
      601 ggccgttacg ctgattttgg acctacgttt gcgtgcgaaa aattgcgcga gatacacggt
      661 ttatctttat ccgttgaaac tctcagaaag tggatgatag aagaggggtt atggcgtgaa
      721 cgccgtcgta aaattgcccg tatatatcaa cgccgcatgc gacgaccatc ttacggtgaa
      781 ctgatccaga ttgatggctc acctcatgac tggtttgaaa atcgaggccc cagatgtaca
      841 ctgatcgttt tcattgatga tgccaccagt gcgttgatgg cgttgcgttt tgtgcctgct
      901 gaaacaaccc gggcttacat ggaaaccctc cggggttacc ttaatgatca tggcgtaccg
      961 ctcgctctct actctgatag acacagtata ttcagggtaa ataacccaga gcgggaaggt
     1021 gagctgaccc agttcactcg tgcgataaag acactgggca tcgagccaat ccatgccaac
     1081 agcccgcagg caaaagggcg ggtagagcgc gccaatcaga cactacagga caggctggtc
     1141 aaagaaatgc ggcttcagaa tatcagtgat attgaaacag caaatgcatg gttgccgacc
     1201 tttattgaag cctataacaa ccggttcgct acgtcgcctc gtactactga taatgctcat
     1261 cttgatgtgc accattctga agaggaactg ggttatatct tcagcctaca ggcgaagcgc
     1321 gttctgtcta aaaatctcac tttccagtac aaaagcagtg cgtttcaggt acgcagtgag
     1381 ggccggggat atcgacttag gcattcggtt gttactgtat gcgagaactt tgacggtgaa
     1441 attaacgttc tgtatgacgg gaaagcgctg ggctgggaaa agtatgttga tggcccggag
     1501 cctataccac tggatgatga aaagagtgtc catgaacgag tggataatgc ccgtattgat
     1561 ttacgctcaa aatactatgt taaacctaaa gctgaccatc cctggcttac gcgccgaacg
     1621 caaagtcatc agcaagttaa gcccccgaag ttacctaaaa agaagcctga tcccgataaa
     1681 aaagattgaa accaagatcg attcggttga gtgcatatcc attcataggg tagattctta
     1741 agtcgcgttt ctggtgttca ttttcgggtg gtttgttact tgttttaccg gggatatgcc
     1801 agaaacgcgc tgagtcagtc tgggcggtgc gcgtaatgag gcgttatggt aaatagccta
     1861 tgctaatgtc cgctaagagc aagaagcgga agttggcagt tttgtggact gtccccacaa
     1921 aagtgactac agaaatagtt gcaattcata attgatcatg ggttgtcagt taaactcgtg
     1981 gcgatttaaa tagactaatt gggagtgcgt ccattactta tatcttgtaa tgttaactat
     2041 cagaaatgat acaaagataa tatgtcttta aagaaaaggc tgatggcgaa aagtggcccg
     2101 atgagggcca caatacggct gtcacttaga cgtaaatatc aatggtgcca gcggtatttg
     2161 tatcgtcttt tttctcttct tttttatcag gctgaactgt cgcgtcttca ttctttttct
     2221 ctgcctgctg ccttaacaac tgctccagtt gagcccagag gctttcaatt tgcttctgta
     2281 ccaatgcagc catttctttt ttctgctgtg tcgtcatccc ctcttccgat gagattttcc
     2341 caagcttttc agtcagcacc tgaatttgtc ttgtgatttt ggctatttct gatgttcctt
     2401 ccggggcgga gttgtttgaa ataacggttg aggtatttcc ctgaattgtg acagacatag
     2461 atttctcctt ttaaaaaagc actatcggca tgcacaaaaa aatctttaat cgtatttctt
     2521 gtgtcattaa ttgtttgatg ttcagattgt tttcctcgcg ggctggcgcg cctcagaaag
     2581 taaagcttgt tgacaggggt aaacgttcgg caataatttt ctgccacatg cgggtgttgc
     2641 ataaaacgtg ttacgttcct ttatcgacag gtcaggtcac cgctcacccg ccgacgagaa
     2701 agcaacactg acatgctaaa gcaaaaaata gatgaataag ttgagttgtg catatgtagc
     2761 ctgaccgtca caaagtatat ggtgtctgta ccagtaagat gatggccgga ctctttaaaa
     2821 acgagctgac ctgcacaata caggatggac ttagcaatgg ctgctcctgg cacaaagcgg
     2881 acagtgatca ccgttcttac gactactttc tgacttcctt cgtgacttgc cctaagcatg
     2941 ttgtagtgcg atacttgtaa tgacatttgt aattacaaga ggtgtaagac atgggtagca
     3001 ttaacctgcg tattgacgat gaacttaaag cgcgttctta cgccgcgctt gaaaaaatgg
     3061 gtgtaactcc ttctgaagcg cttcgtctca tgctcgagta tatcgctgac aatgaacgct
     3121 tgccgttcaa acagacactc ctgagtgatg aagatgctga acttgtggag atagtgaaag
     3181 aacggcttcg taatcctaag ccagtacgtg tgacgctgga tgaactctga tggcgtattt
     3241 tctggatttt gacgagcggg cactaaagga atggcgaaag ctgggctcga cggtacgtga
     3301 acagttgaaa aagaagctgg ttgaagtact tgagtcaccc cggattgaag caaacaagct
     3361 ccgtggtatg cctgattgtt acaagattaa gctccggtct tcaggctatc gccttgtata
     3421 ccaggttata gacgagaaag ttgtcgtttt cgtgatttct gttgggaaaa gagaacgctc
     3481 ggaagtatat agcgaggcgg tcaaacgcat tctctgaacc aaagcatgac atctctgttt
     3541 cgcaccgaag gtgacacttc tgctttgcgt tgacaggaga agcaggctat gaagcagcaa
     3601 aaggcgatgt taatcgccct gatcgtcatc tgtttaaccg tcatagtgac ggcactggta
     3661 acgaggaaag acctctgcga ggtacgaatc cgaaccggcc agacggaggt cgctgtcttc
     3721 acagcttacg aacctgagga gtaagagacc cggcggggga gaaatccctc gccacctctg
     3781 atgtggcagg catcctcaac gcacccgcac ttaacccgct tcggcgggtt tttgttttta
     3841 ttttcaacgc gtttgaagtt ctggacggtg ccggaataga atcaaaaata cttaagtagc
     3901 gcgcagggat aagagggatg gtcccttaaa ggggagagct aattatccgg aaggattctg
     3961 atgatgaaca tcgaagaact gcgtaaaatt ttttgtgaag atggcctcta tgctgtgtgc
     4021 gttgaaaatg gaaatcttgt tagtcattac cgcattatgt gtttgcgaaa gaatggggct
     4081 gcgttaatta attttgtgga tgctcgggtc acggacggat ttatcttgcg cgaaggtgag
     4141 tttgtcactt cattacaggc attgaaagag atcggaataa aagctggctt ttctgctttt
     4201 tcaggagaat aaactcatct acaatcttgc gcggggctga actcccgctg agtaacaccg
     4261 tgccaccgga gaaaaccgat ggcacgcaac gcaaaatatt acaattctga taattcgccc
     4321 gttcttgcct gcacgcacgg gcggtattct cacgcattca agtctgaatg gttccagcac
     4381 cctccatgca ctgcagaaca ggccgaatgg ctgattcatt cttaccgcag gcgcgggttc
     4441 gaggttaaga aagctctcag tctcgactat cggcactgga taatctctgt caggctgcct
     4501 tattccgaac gcccaccacg tgcgtcccgc actttccagc aacggatctg gaggtaacgt
     4561 gcgggtatta cttaaacctg ttctggtgcc tgagcttggg ctggtggtcc ttaagccggg
     4621 ccgtgaatcc atacagatat ttcataatcc tcgagtgctg gtggaaccgg aaccaaaaag
     4681 catgcgtaat ctgccatccg gagtcgttcc tgccgttcgc cagccgctgg cggaagacaa
     4741 aacattgctg ccgtttttta gtaacgaacg ggtgattcgt gctgctggcg gcgttggcgc
     4801 attgtccgac tggctattac gtcatgttac atcctgccag tggcctaatg gcgattacca
     4861 tcacactgaa acagtcattc accgttatgg taccggcgca atggtgttgt gctggcactg
     4921 cgacaaccaa ctgcgtgacc agacatcgga atcactggag ctgcttgctc aacaaaatct
     4981 gacagcatgg gtgattgacg tcatccgtca cgcaataagc ggtacgcagg agcgggaatt
     5041 atctttggct gaattatcct ggtgggcggt ctgcaatcag gtggtggatg cactacctga
     5101 ggctgtatcg cgtcgttcgc tgggattacc agcggaaaaa atctgctcgg tgtaccgcga
     5161 aagcgacatc gtaccgggag agcagaccgc caccagcata ttgaaacaac gcacaaaaaa
     5221 tcttgcaccg ttgccttacg cccaccagca acaaaaatca ccacaggaaa agacggtggt
     5281 aagcatcacc gttgatccag agtctccgga atctttcatg aagctgccta aacgtcgccg
     5341 ctgggttaag gagaaataca cacgttgggt taagacacag ccgtgtgctt gctgcggtat
     5401 gccagccgac gatccgcatc atctgattgg tcacgggcag ggcggaatgg gaacaaaagc
     5461 acatgatctc tttgtgttgc ctttgtgcag aaagcatcac aacgagctgc atacggatac
     5521 agtggcattt gaagataagt atggctccca actggagctg atatttcgtt ttatcgatcg
     5581 cgcgctggca attggcgtac tggcgtaagt ggagaacgag catgaacctt gaagccttac
     5641 caaaatatta ctccccaaaa tctccaaaat tgagcgatga cgctccagcg acaggcaccg
     5701 gttgtttaac aattacggat gtaatggcag cgcaggggat ggtgcagtcg aaagcaccac
     5761 ttgggttggc cttatttctg gcaaaagttg gtgttcagga ccctcagttt gcgattgaag
     5821 gcctgctaaa ttacgcgatg gcactggata acccgacatt gaacaaattg agtgaagaaa
     5881 tccggttaca gattattcct tacctcgtga gttttgcctt tgctgattac tccaggtctg
     5941 cggcaagtaa ggctcgctgt gagcattgtt caggtacggg attttataat gtattgcgcg
     6001 aagtggtgaa acactacaga cgcggggaat ctgtaatcaa ggaagaatgg gtgaaggaac
     6061 tatgtcagca ttgccatggt aagggcgaag ccagcacagc gtgcagaggg tgtaagggta
     6121 aagggattgt tctggatgaa aaaagaaccc ggtttcatgg cgtaccggta tataagattt
     6181 gtgggcgttg taatggaaac cggtttagtc gtttaccgac cacgctggca cgacgtcatg
     6241 tccagaagct ggtaccagac ctgaccgatt atcagtggta taaggggtat gcggacgtca
     6301 ttggtaaact ggtaacaaag tgctggcagg aagaagcata cgcggaagcg caattgagga
     6361 aggtgacgag ataaatgatt tttgctgaag atggcgacat gatgtttgca tttttcaaaa
     6421 aatatggata aaattttttc aacgatgggc tttgtatacc cgacgttaag aaaaagtaga
     6481 aaacccgctg atgagcgggt tttgtgcttt aaatggggca atggtaatgt tgaatctcat
     6541 cccgggactc atgtctgtta acttattatt tagctggtga cttggttatt tgcctgatgt
     6601 ttaaaatgtt ttcttccagt acaatgtccc taaacacaat gagtctgctt attatattat
     6661 tagcagagct attacggcca aagtacagca taagctttta aagccaatca accagtcatc
     6721 aagacagacg gggttattca taaaaactct ccatgtgtga tccgatgggg cctgaaatta
     6781 aagctttaat atagctcatg aaaggtaaac attggcagct gaagggccac gcagaccatt
     6841 tatccggcaa aattccacgc gtaatccggt ggtaatttct tctgcatcgc ggagattgag
     6901 cgctgaaaca tgaagctgga catcgatacg accatcggat ggggtgataa gacccttgcc
     6961 gcttttgccg tcaaaggttt tgacaattcc tgtcatttta cgggacaaaa aaattcctta
     7021 atactgataa cttggcgcac tatacacacg ttcctgaaga aagctatagt tttttgatgg
     7081 ggttgaagat ggctggatgt ctaaaataaa cattgcttca tatgttcaac tatgcgttaa
     7141 tgattgcgtc ggtttgaaga acagacgata tacgaagtag tttactaaag cagttctcat
     7201 ttcaggtgtt attcacttat tccttctttg agtctctcca attaagtacg aagtcgtttc
     7261 tgttatgcaa accatttatg ccgaaaggct caagttaagg aatgtagaat gtcaaataaa
     7321 atgactggtt tagtaaaatg gtttaacgct gataaaggtt tcggctttat ttctcctgtt
     7381 gatggtagta aagatgtgtt tgtgcatttt tctgcgattc agaatgataa ttatcgaacc
     7441 ttatttgaag gtcaaaaggt taccttctct atagagagtg gtgctaaagg tcctgcagca
     7501 gcaaatgtca tcattactga ttaaaattca tcgctcgtct gtatacgata acgaagaagg
     7561 ctgatgcctg agtagagata cggacagagt agtgaatatt ggatctcttt aataaaaagt
     7621 aaggaggtcc aatacatgaa acaatggcta gcatatttgg caaaatctta atcaggaaaa
     7681 gtatgctaac cattgtggtg aagtgcaggt ttgctgcatg aatagtttta cagcagaagc
     7741 taactgctgg catggcaaaa caaagtgcgt aagtggatga ctcccacaaa aagcaccaca
     7801 atctcaaacc cgc
//
LOCUS       DAPHBJ010000202         7804 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.204, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000202 DAPHBJ010000000
VERSION     DAPHBJ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.204"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(173..436)
                     /gene="cedA"
                     /locus_tag="RHZ76_003865"
     CDS             complement(173..436)
                     /gene="cedA"
                     /locus_tag="RHZ76_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HDV2814419.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            619..2880
                     /gene="katE"
                     /locus_tag="RHZ76_003866"
     CDS             619..2880
                     /gene="katE"
                     /locus_tag="RHZ76_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HDV2814420.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3138..3887)
                     /gene="chbG"
                     /locus_tag="RHZ76_003867"
     CDS             complement(3138..3887)
                     /gene="chbG"
                     /locus_tag="RHZ76_003867"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDV2814421.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEQGIALRADRQMAFDLP
                     VNLRTTQGFSSAFYGEEISESLFLQVLDDAGHRGDRSLEVMCHPAFIDNTIRQSAYCF
                     PRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(3900..5252)
                     /gene="celF"
                     /locus_tag="RHZ76_003868"
                     /gene_synonym="chbF"
     CDS             complement(3900..5252)
                     /gene="celF"
                     /locus_tag="RHZ76_003868"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDV2814422.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSLF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5356..6198)
                     /gene="chbR"
                     /locus_tag="RHZ76_003869"
     CDS             complement(5356..6198)
                     /gene="chbR"
                     /locus_tag="RHZ76_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HDV2814423.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6206..6556)
                     /gene="chbA"
                     /locus_tag="RHZ76_003870"
     CDS             complement(6206..6556)
                     /gene="chbA"
                     /locus_tag="RHZ76_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HDV2814424.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRLALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6607..>7804)
                     /gene="chbC"
                     /locus_tag="RHZ76_003871"
     CDS             complement(6607..>7804)
                     /gene="chbC"
                     /locus_tag="RHZ76_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HDV2814425.1"
                     /translation="FGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMA
                     PFFIGMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIG
                     LVVAEMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNF
                     HQIIMDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIA
                     TYQQYGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVA
                     KLALPSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIA
                     PWTMPTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDI
                     ANALKF"
BASE COUNT         2082 a         2061 c         1754 g         1907 t
ORIGIN      
        1 caaaacttaa ccattttaaa ataaataaac aattaaagaa aaaagatcac ttatttatag
       61 caatagatcg tcaaaggcag ctttttgtta caggtggttt gaatgaatgt agcaacgaaa
      121 tacagaattt caggtcatgt aactcccggc aaaaccggga ggtatgtaat ccttactcag
      181 tcacttcccc ttcctggcgg atctgatttg cccaacgttg ggcagattca ggcacagtaa
      241 acgccggtga gcgcagaaat gactctccca tcagtacaaa cgcaacatat ttgccacgca
      301 gcatccagac atcacgaaac gaatccatct ttatcgcatg ttctggcggc gcgggttccg
      361 tgcgtgggac atagctaata atctggcggt tttgctggcg gagcggtttc ttcattactg
      421 gcttcactaa acgcatatta aaaatcagaa aaactgtagt ttagccgatt tagcccctgt
      481 acgtcccgct ttgcgtgtat ttcataacac cgtttccaga atagtctccg aagcgggatc
      541 tggctggtgg tctatagtta gagagttttt tgaccaaaac agcggccctt tcagtaataa
      601 attaaggaga cgagttcaat gtcgcaacat aacgaaaaga acccacatca gcaccagtca
      661 ccactacacg attccagcga agcgaaaccg gggatggact cactggcacc tgaggacgga
      721 tctcatcgtc cagcggctga accaacaccg ccaggtgcac aacctaccgc ccctgggagc
      781 ctgaaagccc ctgatacacg taacgaaaaa cttaattctc tggaagacgt acgcaaaggc
      841 agtgaaaatt atgcgctgac cactaatcag ggcgtgcgca tcgccgatga tcaaaactca
      901 ctgcgtgccg gtaaccgtgg tccaacgctg ctggaagatt ttattctgcg cgagaaaatc
      961 acccactttg accatgagcg catcccggaa cgtattgttc atgcacgcgg atcagcagct
     1021 cacggttatt tccagccata taaaagctta agcgatatca ccaaagcgga tttcctctca
     1081 gatccgaaca aaatcacccc agtatttgtt aggttctcta ccgttcaggg tggcgctggc
     1141 tctgccgata ccgtgcgtga tatccgtggt tttgccacca agttctatac tgaagagggt
     1201 atttttgacc tcgttggcaa taacacgcca atcttcttta tccaggatgc gcataaattc
     1261 cccgattttg ttcatgcggt aaaaccagaa ccgcactggg caattccaca agggcaaagt
     1321 gcccacgata ctttctggga ttatgtttct ctgcaacctg aaactctgca caacgtgatg
     1381 tgggcgatgt cggatcgcgg catcccccgc agttaccgca ccatggaagg cttcggtatt
     1441 cacaccttcc gcctgattaa tgccgaaggg aaggcaacgt ttgtacgttt ccactggaaa
     1501 ccactggcag gtaaagcctc actcgtttgg gatgaagcac aaaaactcac cggacgtgac
     1561 ccggacttcc accgccgcga gttgtgggaa gccattgaag caggcgattt tccggaatac
     1621 gaactgggct tccagttgat tcctgaagaa gatgaattca agttcgactt cgatcttctc
     1681 gatccaacca aacttatccc ggaagaactg gtgcccgttc agcgtgtcgg caaaatggtg
     1741 ctcaatcgca acccggataa cttctttgcc gaaaacgaac aagcagcatt ccatccaggt
     1801 catattgttc ctggtctgga cttcaccaac gatccgctgt tgcaggggcg tttattctct
     1861 tataccgata cacaaatcag tcgtcttggc gggccaaatt tccatgagat tccgattaac
     1921 cgtccgacct gcccttacca taatttccag cgtgacggca tgcatcgcat ggggatcgac
     1981 actaacccgg cgaattacga accgaactcg attaacgata actggccgcg cgaaacaccg
     2041 ccggggccga aacgcggcgg ttttgaatca taccaggagc gcgtggaagg caataaagtt
     2101 cgcgagcgca gcccatcgtt tggcgaatat tattcccatc cgcgtctgtt ctggctaagt
     2161 cagacgccat ttgagcagcg ccatattgtc gatggtttca gttttgagtt aagcaaagtc
     2221 gttcgtccgt atattcgtga gcgcgttgtt gaccagctgg cgcatattga tctcactctg
     2281 gcccaggcgg tggcgaaaaa tctcggtatc gaactgactg acgaccagct gaatatcacc
     2341 ccacctccgg acgtcaacgg tctgaaaaag gatccatcct taagtttgta cgccattcct
     2401 gacggtgatg tgaaaggtcg cgtggtagcg attttactta atgatgaagt gagatcggca
     2461 gaccttctgg ccattctcaa ggcgctgaag gccaaaggcg ttcatgccaa actgctctac
     2521 tcccgaatgg gtgaagtgac tgcggatgac ggtacggtgt tgcctatagc cgctaccttt
     2581 gccggtgcac cttcgctgac ggtcgatgcg gtcattgtcc cttgcggcaa tatcgcggat
     2641 atcgctgaca acggcgatgc caactactac ctgatggaag cctacaaaca ccttaaaccg
     2701 attgcgctgg cgggtgacgc gcgcaagttt aaagcaacaa tcaaggtcgc tgaccagggt
     2761 gaagaaggga ttgtggaagc tgacagcgct gacggtagtt ttatggatga actgctaacg
     2821 ctgatggcag cacaccgcgt gtggtcacgc attcctaaga ttgacaaaat tcctgcctga
     2881 tgggaggcgg cgcaattgcg ccgcctcaaa tgatttacat agtgcgcttt gtttatgccg
     2941 gatgcggcgt gaacgcctta tccggcctac aaaactgtgc aaattcaata tattgcagga
     3001 aacacgtagg cctgataagc gaagcgcatc aggcagtttt gcgtttgtca gcagtctcaa
     3061 gcggcgcagt tacgccgcct ttgtaggaat taatcgccgg atgcaaggtt cacgccgcat
     3121 ctggcaaaca tcctcactta cacatcccga taactcccca accgataacc acgctgagcg
     3181 atagcacctt tcaacgacgc tgatgtcaac acatccagct ccgttaagcg tggaaaacag
     3241 taagcactct gacggatagt attatcgata aacgccggat gacacatcac ctccagcgaa
     3301 cgatcacccc gatggcctgc gtcatccagc acttgcagga acagcgactc actgatctct
     3361 tcaccgtaga atgcactgct gaatccctgg gtagtgcgca ggttgaccgg caaatcaaac
     3421 gccatctgac gatccgcccg caacgcaatg ccctgctctg ccgcaaacct ggcaacaatc
     3481 gggaaaatct gcgggaacat atgcacatga tgatggctat caagatgcgt aggtttgcgt
     3541 ccaaatagct caatgaaacg caaatactga ctggcaagct cctgagtaat ttcttccagc
     3601 ggtaaagcat cttcttctgc caactgccag atccattttc ccagcacacc atcgcgggtt
     3661 aaccccggca tagctgtcag tggcttaccc atagtaagga caaagtgcat ccctatggcc
     3721 agactcggtt catcacgact caactgcacc gcatggtcaa tagcctgccc attcaccagc
     3781 gccgtcgtcg acgtgacaat cccattgcga caggcctcga taatgccgta gttctgacct
     3841 ttgcttaagc caaaatcatc ggcattaaca atcagtaagc gttccataat cagcctcggt
     3901 taatgtgctt ttttaagctc tgcgatgcag tcggcaaagt ttggcagcca tttctcgtgc
     3961 gccagaatca tctcgcgtgc cagcagctca gcatcgcgat cggaatgcac caacggacta
     4021 aggtttagcg ccagtaacac atcgttaaat tctccgctaa gtgcggcgtt gctggcagca
     4081 atctcgaagc ctttaatggt gtgaatcagc cccatcactt tatcatcgaa atgcgtaatg
     4141 cgcggatgtg gcgtcgcgcc atcgcgcccc agcttacagg tcatttctac cgcccagtct
     4201 gccggaatat tatcaatatg cccatgatgc gggatattaa cgtaatgttc agcttgcttg
     4261 tcgttgtaga tagcgttgat cacttcgcac gctgcatcag agtaataagc cccaccgcgc
     4321 tgttccagtt ctttcggctt aactttcaac tccggatttt tatacagctc aaaaagttgt
     4381 ttctcgactt tctgtactac ctgtgctcgt gcgccgcctt tgtagtattc gcccatttca
     4441 atagccagca tctctttctg cttgaagtaa tacagcagat aagaacatgg cagcagattt
     4501 aacgaacgaa ttaagccttc gctaaatggc agatcgaaaa tatttttaac gccagatgct
     4561 tttaactgcc ctgacgccac accatcaagc aattcggcaa agagcgactt gccatttacc
     4621 agcacatcct taatgaacac catatggttg aggccgaaca gatcgataga taaatcatcg
     4681 ctgtctttca gcatcagaac atcgcgaata aacatcttca tgccgatcgg aatattacac
     4741 acgccgataa agcgtttaaa tccagtatga cgataaacgg cttcagtgac cattcccgcc
     4801 gggttagtga agttaatcac ccatgcattc ggacaaagtt cttcgacatc ttttacgatg
     4861 tcaaaaatca ccggaatggt acgcagacct ttaaacaaac cgcccgcgcc gttggtttcc
     4921 tgaccaagat aaccatgact taatggaata cgttcatcca gttcacgcgc cggtaattgg
     4981 ccaacgcgca gttgggtagt aacgaaatca gcatctttca atgcttcgcg gcgatccagc
     5041 gttttataaa gcttcatcgg gacgccagcg ttatcaatca tccgttggca gagatcgaaa
     5101 ataatatcca gtttagcttt accaccttcg acatccacca gccataattc gctgaccggc
     5161 aattcgtggt atcgcttaat aaatccttcc agtaactccg gggtatagct gctcccgcca
     5221 ccaatagtga cgacttttaa tttctggctc ataatttctc ccttcagtac agaatactga
     5281 tatctggcat atctgccccc cggacataaa taatccagca acaggacaga tatgtgaatt
     5341 gtcaggtata acgacttact gattaaattc agtcaatttc ttacgatagc tcttaggcgt
     5401 aaaggacgtt aatttcttaa acgttttaat aaacaaactc gggctactat aaccggcctc
     5461 aaacgcaata tccgtcactg aatagttggt catttccagt tgttttttgg caaaattaat
     5521 acggatttca ttaataatct gcattggcgt tttgccataa tatcgttgag tcgcccgcgt
     5581 caaatattcc tgtgatttgg ctgacaacgt caccatattc tccagcgccg attcactaaa
     5641 ctgctcttta tcatgcatct tttctaccgt acttttcagc cactgcggta catcatcaat
     5701 cacctgttct tcgcggtaat gacgtaaacg gttaatgaca taaaaagtaa ccatctcaac
     5761 aaactcttcc agccctgttt cgcggaaatt caatgaagaa atcactgttt ccacataggt
     5821 gagaaaagcg ttgttggtcc ggtatacctg cgaagctaca aagcaataag gcaacaacgg
     5881 caggtaatgc tgctcaaaaa agcgtttact gatcccaacg ttcaatatac gcgtggcacc
     5941 aaactcatag aaactttgat ggtgcgaacc taacggaata aaaacaaaat caccccgttc
     6001 cagtaacacg cgcttaccgt taatctcctg gaaataacgc ccggttaata ccagagtaaa
     6061 ttcataatag tcgtgctgat gcagtccgct gatactctca gttttgttat agataaacac
     6121 atggaagttt ttgccattaa acaactgctg ttctcgggca gtggcaattt ccggcgcgtt
     6181 aatcactggc tgcatcatcg actccttatg ccttcagttt ttcatgaagc tcaattaatt
     6241 cagtaatcag ttcacgcgca agcatggacg tcattaaatg atcctgagcg tgaaccagca
     6301 ccagactcac tttcatctta ccttcgcccg catcgccttc aatcagtttc gtctgtacca
     6361 gatgcgcttc attcaatgcc agccgtgact gatccatcat ggcttttgct gcggcaaaat
     6421 cgccctgctt cgcctgtttc agtgccgcat acgccaggct gcgcgcttgt ccggagttga
     6481 tgataagccc catcaccact tcttccagct cttcagcttc cgtttgcgta tcgggaatgt
     6541 tgtcgagatc catcatacat cgttcctctc tctgcttacc ggcacgatta cccgtaccgg
     6601 catcgattaa aatttcaggg cgttagcgat atcttcttcg ctctcttctt tatcaatcgc
     6661 attttgtgct ttgttagcca ccacaacaaa gggcagataa attaacgttg cgatgccaag
     6721 gttgaagagt gcgaccagca atgcggcgac gctaccgttg gtgttaaaga aggctcccag
     6781 accggttggc atggtccacg gtgcaatatt ggtcaccgga ggaatgatgc ccatgtagta
     6841 cgctgcgagg gtgattgccg ccagaatcgg ttgtaccagt acaaacggga taaacatcac
     6901 cgggttcatg ataattggca gaccaaacag aatcggttcg ttaatctgga agatgccgga
     6961 cggcagcgcc agttttgcca cctgacgata atcagcacgg cgagaggcga taaagatagc
     7021 caggatcagg cctaaagtcg caccactgcc cccaaggaaa ataaaggagt ccagcatcgg
     7081 cttggcccag atatggaagg tcttaccggc tgccagcgcc gcttcgacgg aaccatattg
     7141 ctgataggtc gcgatatttt ccagcgccca cggcgtcata atgccgttgt ccagtgcggt
     7201 cagcgccagc gcgccatgaa taccgaagaa ccagagcagt ggaacaaaga tcacataggc
     7261 ccagcccacc acgctaccca acgatgccag tggggttgag atggtatcca taatgatctg
     7321 atggaagttg gtgccccagg tattcaacgc ccaggcaata atccccatca cggaaagaat
     7381 aataaagccg ggaattaatg ccgagaagga acgcgatact gaagcaggta cgctgtcggg
     7441 cagtttaatg acccaattgc ggcggacaat aaaggtaaac atttctgcca ccaccaggcc
     7501 aataataatc ccggagatga tattcgcccc acctaaccag tttgcgccaa ccgcataggc
     7561 ctcaccgaca ctatatgggg tgacggtcat aaatgctgca acggataaca accccgccgc
     7621 cagcgcatcg actttacgct cttctgccag cgccatgcca ataaagaacg gtgccattaa
     7681 agacattatc cctaatgttc cgttatatac gttgccgcca atacctttca ggccattaag
     7741 tgtttcaatg gttgaggcgt cgaggcgaat acctaaggaa taaaaaaacg acccctcccc
     7801 aaag
//
LOCUS       DAPHBJ010000203         7736 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.205, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000203 DAPHBJ010000000
VERSION     DAPHBJ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.205"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            244..1056
                     /locus_tag="RHZ76_003872"
     CDS             244..1056
                     /locus_tag="RHZ76_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDV2814426.1"
                     /translation="MSMNYQPELKLSTRTEQLASSRDAAMQKFLDGIMLIAEASAICG
                     FSLFNSKIMAPNAFGLPASLAASIEEGRQQIDRKTWNNLFEETGIDRFWNHNQRAEFS
                     ESLRSAPPIASLTVIRSTLRQAVAIRSITLAQGFVDLLCQLDRRYKTNAQQFVMPKKL
                     VLRGIFPDSNMMRYNGFSQDNLFYLNDFENIVCICSNAATPPVGSGLNMYDRLAALRK
                     TDFTGDITDPKGWKCRLFVNGNVHISVECESLHNTLNDLISIYFANQIPAKG"
     gene            1062..2279
                     /locus_tag="RHZ76_003873"
     CDS             1062..2279
                     /locus_tag="RHZ76_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814427.1"
                     /translation="MTDNENLMNGDEQTASNLSEETTKKRPLSPELLKNSPYSSIAKV
                     KYFTEIEQICLDKGISTENLDQFFKAHWLRDKMGGSFARAQEMLAAYKQYVDEVPEEA
                     RAIEIPDQIKEAFSDFTAFIIWYFRLSYTAIQSDSVKIAKAEITQLRHRNAEILEELS
                     QSKEQVTVLNNEKVNLINQLEQQRELASKLGDSLREAEETLATTQSELQYAQNEIQLI
                     QQTVGTLNQQLSERKQELVSQQEYQKQLNDENKAQQVELTALKSQNDHLQRTVSDLKV
                     SVSQLEQDLSSVQTHSSELSSSLAEKDTTLTLVRSELSTAKGENDQLRAEAQRLADES
                     LVAKKVQTDQTEELQQLRNQMISMEATLNAEKTIAESLRGTIKQLTEAMTGAVAIKPK
                     SSGASKPRNKKTT"
     gene            complement(2334..2537)
                     /locus_tag="RHZ76_003874"
     CDS             complement(2334..2537)
                     /locus_tag="RHZ76_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814428.1"
                     /translation="MAYILSMSEQVKLKAFLAAVLDDYKLGAISQQQAVGGITSLVDA
                     IEISDSGKISLMLSEGRKLLRTG"
     gene            complement(2551..3870)
                     /locus_tag="RHZ76_003875"
     CDS             complement(2551..3870)
                     /locus_tag="RHZ76_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1173 family protein"
                     /protein_id="HDV2814429.1"
                     /translation="MGQKFAVFIAYDDEPNTKRYSAEFQTQDEYVKGWQSALKKAHHT
                     SGQKSVISCGCRGKGAKRLYVRSLPNSDTFILIKAANTGTEHDPSCVFFDLDARHTGL
                     KGYASNVVRINNEGTMSIRLGIGMTEKDPPEKSEVPSLPQIQRPEGGQASMTLSGLLS
                     LLWTEAGLNVWYPNMAGKRNDSLVRYRMLEAAKQIRSGRACIGDHLFIGVADSKSKVA
                     SEQVQLLSSAELSDKRLLLLSVLPRYDAEKHEKPLKFLPMRNFGGMPLTFFNSDGHWD
                     SVKRRFPLEYAAWKNGGKVVVFALTSPVSVTSRGISARAHQIVLMLVSDNWIPLDSSY
                     EAIVSRKLDAEHRHYVKPMRYDASASDVFPDFYLLDTRSDKPFPMEVFGMSTPAYLAR
                     KELKKAYYNQEYGAYGWWHWDATEKTEYQELPHFPEARRYNSPESQP"
     gene            complement(3895..4065)
                     /locus_tag="RHZ76_003876"
     CDS             complement(3895..4065)
                     /locus_tag="RHZ76_003876"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814430.1"
                     /translation="MMTYFDSAEDLTISKQRALQELAKHGVVASDIDVFFSELGEREE
                     YNAQEVLIWLGY"
     gene            complement(4194..5585)
                     /locus_tag="RHZ76_003877"
     CDS             complement(4194..5585)
                     /locus_tag="RHZ76_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDV2814431.1"
                     /translation="MLNDETHSSIHKLLVQVASIYDVKDLAAQLNAATGSDWSRASLI
                     RQIKGSVNECRITQEEYHYLRSLLPSRPADYDQKFFRFIDLFAGIGGLRSGFDAIGGK
                     CVFTSEWNQFSRRTYSANWYCEDTEHHFNSDIRDITLSNLPDVSEDQAYASIAASIPD
                     HDVLLAGFPCQPFSIAGVSKKNSLGRKHGFECDTQGTLFFDVARIIRAKQPAIFVLEN
                     VKNLKSHDKGNTFRIIMKALDELGYDVADCDAHGSKDPKIIDGKNFRPQHRERIVLVG
                     FRRDLKLHDGFSLSGVSALYPSRRPTLGELLDAEVDSRYILSPKLWEYLYNYAKKHQA
                     KGNGFGFGLVNPLDKSVVCRTLSARYYRDGSEILLDRGWDHSIGEKDFNDPVNMARRP
                     RRLTPRECARLMGFEQPGKVTFRIPVSDTQAYRQFGNSVIVDVFAAVASLLRSRIEIA
                     VAARLKGEFEAAS"
     gene            5844..6359
                     /locus_tag="RHZ76_003878"
     CDS             5844..6359
                     /locus_tag="RHZ76_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDV2814432.1"
                     /translation="MTLLKKLLLPTLIVLFPAAVLAGQSSFEAKVVKIIDGDTITALD
                     GQNTSIKIRMYGIDAPESKQAFGQKAKQALSSAIAAQNITVIDHGPDIYGRMLGTIWL
                     DGYDINASMVDSGYAWVYRFDGNPIVPNYLKFEASAQKAVKGLWVDPNPVAPWEWRQQ
                     NQKPQKTKSRG"
     gene            6365..7297
                     /locus_tag="RHZ76_003879"
     CDS             6365..7297
                     /locus_tag="RHZ76_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814433.1"
                     /translation="MTSQHPLLKIALNGDGLLSWPNSPGQTMRCPTCMRDVITGPDGD
                     LIHQMEEGANPCTPSVDVVISKAIIELLSTGERLYVNPVKNANRTLAPSFIFLHKDKQ
                     LRPFINTEYQPAGATWRSDKGNRLGFFYMDARASKINKDQFDFIAVIDPSTFQAEFKT
                     LWSMCEFDNPLAALQHLLVSENMSSVWVKWPVKSLQPRKEAVKADYWYDYYSPHYPAQ
                     QAACTATVIGLEGNVSGETIYMLQIAVDRSIVEYQLVKSLGVILLLDNTGNLVSETRP
                     HFDIIIKACISGVRDFVRQNPRMRSVEHQITSAI"
     gene            complement(7505..>7736)
                     /locus_tag="RHZ76_003880"
     CDS             complement(7505..>7736)
                     /locus_tag="RHZ76_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814434.1"
                     /translation="LINSCLSQNLIPAYLQSQFTSLKTMLETGIPTIRNKNAGHGQGA
                     DIKEVPEELASYMLHLTATNLLFLLKCEKNIK"
BASE COUNT         2143 a         1843 c         1764 g         1986 t
ORIGIN      
        1 taatagaaac taaatcctta tatatcaata acttgaattc gccatcacgc acccatagag
       61 agcacccgtc ttttgtcggg ccgtagggtt gttggcccca tagtaactgc atgctaatct
      121 ctttcatgct tagtcactag taaaggcttt actaattgcg atgctacgtg aatattattg
      181 acggaggagg gggttggggc agtgaaaatc tctcagccaa aacagtggga aaaggatcgt
      241 gaaatgagca tgaattatca gccggaactg aagttgagta cccgcaccga acaactcgcc
      301 agtagtcgtg atgccgctat gcaaaaattt ctggacggta ttatgctgat tgccgaagcc
      361 tcagctattt gcgggttttc actatttaac agcaaaatta tggcccctaa tgcttttggc
      421 cttcctgcgt ccctggccgc aagcatcgag gaagggcgac agcaaataga ccgtaagaca
      481 tggaacaatc tttttgaaga aacgggtatt gaccgattct ggaatcacaa ccagcgagcg
      541 gagttcagtg agtctctccg gtccgctccg ccaattgcgt cgttaaccgt aatccgtagc
      601 acattgcgcc aggcagtggc cattcggagc atcacgcttg ctcaagggtt tgttgatctg
      661 ctatgccagc ttgatcgccg gtataaaact aatgcccagc aatttgtgat gccaaaaaaa
      721 ctggtactac gtggcatttt tcccgactcg aacatgatga gatataacgg cttttctcag
      781 gataatctct tctatttgaa cgactttgag aatatcgttt gcatctgctc caacgcagcg
      841 acacctcctg tcggcagtgg attgaatatg tacgaccggc ttgctgcctt aaggaaaacc
      901 gattttactg gtgacattac cgacccgaag ggctggaagt gccgattgtt cgtaaatggt
      961 aatgtccata tttcagttga gtgtgaaagc ctgcataaca cactgaatga cctgattagc
     1021 atttattttg ccaaccagat cccggccaaa ggataagaat gatgactgac aatgaaaatt
     1081 tgatgaacgg ggatgaacag acagcgtcca atttaagtga agagacaaca aaaaagcgcc
     1141 ctctcagccc cgaactactg aagaattctc cttattcatc aatagctaag gttaaatatt
     1201 tcaccgagat tgaacaaatc tgcctcgata agggcatcag caccgaaaac ctcgatcagt
     1261 ttttcaaagc ccactggctg cgggataaaa tgggagggtc ttttgcccgc gctcaggaga
     1321 tgctggctgc ctacaagcaa tacgtggacg aggtgcccga ggaagcgcgt gcgattgaaa
     1381 tccctgacca gattaaagaa gcatttagtg attttacggc gtttatcatc tggtacttcc
     1441 gcttaagcta tacggctatc cagagtgata gcgtgaaaat tgcgaaggca gaaattactc
     1501 agttaagaca tcggaatgct gaaattctgg aagaactgtc acagtcgaaa gaacaggtta
     1561 cagtactgaa taacgaaaaa gttaacctga ttaaccagct ggaacaacag cgtgaactcg
     1621 ccagtaaatt gggagattca cttcgggaag cagaagaaac gctggctaca acccaaagtg
     1681 aattacagta tgcgcaaaac gagattcagc ttatacagca gacagtaggg acactgaacc
     1741 aacaactgag tgagcgcaag caggaactgg tctcccagca ggagtaccag aagcagctga
     1801 atgatgagaa taaagcgcag caggtagagc tgacagccct caaaagccag aatgatcact
     1861 tacaacggac cgtgtctgat ttaaaagtca gtgtcagtca gcttgaacag gacctttcct
     1921 cagtacagac ccattcctct gaattgagct catctttagc cgaaaaggac acgaccctga
     1981 cgctcgtgcg gtcggagctc tcaacagcta aaggggagaa tgaccaacta cgagctgaag
     2041 cacaacgctt agctgatgag agcctggttg ccaaaaaagt gcaaacagat cagacagaag
     2101 agcttcaaca gctcaggaat cagatgattt ccatggaagc gacactcaat gcggaaaaga
     2161 ccattgccga gtctttacga ggaacaatca agcagcttac cgaagctatg accggcgcag
     2221 ttgccatcaa acctaaatcc tctggggcat caaaaccgcg aaacaaaaag acgacataac
     2281 tgctcccgcc aggaatttgc aatagactta aattcctggc gtccacaggc tctttatccc
     2341 gttcgcagga gtttacgtcc ttccgacagc attaggctga ttttccccga atcactgatc
     2401 tcaatagcat caaccaggct agtgatcccg ccaacagctt gctgttgtga aatagctccc
     2461 agtttgtagt catcgagtac tgccgccaaa aacgccttca gtttaacctg ctcactcatc
     2521 gaaagtatgt acgccatttt tagccccatt ttaaggttgt gattcgggtg aattgtatcg
     2581 tctagcttcc gggaaatgag ggagttcctg gtattcagtc ttctccgtag catcccagtg
     2641 ccaccatcca taggccccat attcctggtt gtaatacgcc ttcttaagtt cctttcgcgc
     2701 aagataggca ggtgtcgaca tcccgaaaac ttccatcggg aacggcttat cacttcgtgt
     2761 atccagtaaa taaaaatctg ggaaaacatc gctcgcactg gcgtcatacc gcataggctt
     2821 aacatagtgc cggtgttcgg catcaagttt acgggacact atcgcttcgt aggaggagtc
     2881 cagaggtatc cagttatcac tcaccagcat aagcactatc tgatgagccc gtgcagagat
     2941 acctctactg gtcaccgata cgggagatgt aagagcaaaa acaacaactt tgcccccatt
     3001 tttccaggcg gcatattcca gagggaatcg ccgcttcacg ctatcccagt gcccgtctga
     3061 gttaaagaag gtaaggggca taccgccgaa gttacgcata ggtagaaact tcagcggctt
     3121 ctcatgcttt tctgcgtcat agcgcggcag tactgatagc agcagcagtc gtttgtcgct
     3181 cagttccgcc gacgacagca gttgaacctg ctcactggcc actttgcttt tagagtccgc
     3241 caccccgata aaaagatggt cgccaataca tgctctaccc gatctaatct gctttgcggc
     3301 ttccaacatc cggtaccgaa ccagcgaatc atttcgcttc ccggccatat tcggatacca
     3361 gacattaaga ccggcttcag tccacaacag actcagaagt ccggacaggg tcattgaagc
     3421 ctggccacct tcaggtcgct ggatttgggg cagggaagga acctcagact tttccggcgg
     3481 atctttctcc gtcatgccta ttccaaggcg gattgacatg gtcccctcat tgttgatgcg
     3541 tacaacattg ctggcataac cctttaaccc tgtatgcctg gcatcaaggt cgaaaaacac
     3601 acacgatgga tcatgctcag tgccggtatt tgcagcctta atcaggatga acgtatcact
     3661 gtttggtagt gagcggacgt agagacgctt tgcgcctttt ccccgacatc cgcagctgat
     3721 cacagatttc tgcccggacg tgtggtgtgc tttcttcagg gccgactgcc atccctttac
     3781 atactcatcc tgtgtctgaa attcagccga gtatcgtttc gtgtttggtt cgtcatcgta
     3841 agcaataaaa actgcaaact tctggcccat gggacctcct gaaatttaaa aatactaata
     3901 gcctagccat atcaatactt cttgcgcgtt gtactcttcc ctctcaccca actcggaaaa
     3961 aaatacgtct atgtcgctgg ctacgacgcc atgttttgca agctcttgca gagctctctg
     4021 ttttgaaatg gtgaggtctt ctgctgaatc aaaatacgtc atcattacct cctgatgaag
     4081 gagttgactg aagcaggcct ttctctccct gcccaggttt ttttctgtgc aaaagttaca
     4141 atattagtga aataaaccat tacgttaacc tgcaaaatca cacagaagaa ttactatgat
     4201 gccgcttcaa attctccctt gagacgagcc gcaacggcga tctcaatacg gcttcgaagc
     4261 aaactggcca cggccgcaaa tacgtcgacg atgaccgaat tgccgaactg gcgataggcc
     4321 tgggtatcag agactggaat gcgaaaagta acttttcccg gttgctcgaa tcccataagg
     4381 cgggcacatt ctcgcggggt taaacggcgc gggcgccttg ccatatttac aggatcatta
     4441 aagtcctttt cgcctatgct gtgatcccat ccacggtcta gcaaaatttc agagccatca
     4501 cgatagtacc gtgcgctgag tgttctgcaa acaactgatt tatcaagtgg atttaccagt
     4561 ccgaagccaa atccattacc ttttgcctgg tgctttttag cgtagttata aaggtactcc
     4621 cagagctttg gagaaaggat gtaacggcta tcgacctcag cgtccagcag ctccccaagg
     4681 gttggccgtc gtgaaggata aagagccgat acaccactta acgaaaagcc atcatggagc
     4741 ttcagatcgc gcctgaatcc aaccaacacg atacgttccc ggtgctgagg cctgaagttc
     4801 ttaccgtcaa ttattttggg gtcctttgat ccgtgagcat cgcagtcggc aacatcgtag
     4861 ccaagttcat caagcgcttt catgatgatg cggaacgtat ttcccttatc gtggctcttc
     4921 agatttttta cgttctcaag aacgaaaatg gcaggctgtt tggctcggat gatccgggcg
     4981 acatcaaaaa aaagggtccc ctgggtgtcg cattcaaagc cgtgttttcg gcccaaagag
     5041 ttctttttgc tgacaccggc aatactgaac ggctggcatg ggaaaccggc gagcaaaaca
     5101 tcatggtcag gaatggatgc agcgatagaa gcatacgcct ggtcttccga cacatcggga
     5161 agattgctga gtgtaatgtc ccggatatct gagttgaaat ggtgctcggt gtcttcgcag
     5221 taccagtttg cactgtatgt ccggcgggag aactgattcc attcgcttgt aaacacgcat
     5281 ttcccgccaa tggcgtcaaa accactccgg aggccaccga tcccagcaaa caagtcaata
     5341 aaacggaaaa atttctggtc atagtctgct gggcgtgagg ggagcaacga tcgaagataa
     5401 tgatattctt cctgggttat tcgacattca ttaactgaac ctttgatctg gcgaataagt
     5461 gatgccctcg accagtcgct gccggttgca gcattaagtt gcgcagccaa atcttttacg
     5521 tcatagatac ttgcaacctg aaccagtaat ttatggatag aggaatgtgt ctcatcattt
     5581 agcaatgcag ttattgcagg ctcatttctc gcgctgtaca tccttactcc gtgaaatgtg
     5641 attaatttag gttctcagac taccacgcgc agaaaattca atttttcttt aaagcccttt
     5701 attttcatct cctgaaataa attttttatc cacttaaatt gcctccgtta acggaaattt
     5761 gcccgggttc taattggtac aataagcact tctaatcaca cgactaattt gttactacaa
     5821 accatctaat tcagggagaa tgtatgactc tgttgaaaaa gttacttttg ccgacattaa
     5881 ttgtcctttt cccggcggca gttctggctg gtcagtcttc atttgaagca aaagtggtaa
     5941 aaattatcga tggggacacc attaccgcgc tcgacggtca gaatacaagc atcaaaatcc
     6001 gtatgtacgg tattgatgcc cccgaaagta agcaggcctt tggacaaaag gctaaacaag
     6061 cgttgagctc cgcgattgct gcacagaata ttacggtaat agaccacgga cctgatattt
     6121 acggcaggat gttggggact atttggcttg atggttatga catcaacgct tcaatggtag
     6181 acagtggtta tgcatgggta tatcggtttg acggtaatcc catcgtccca aactatctta
     6241 aattcgaagc ttcagctcag aaagccgtta aaggtctctg ggtagatcct aatcccgttg
     6301 ctccttggga gtggcgccag caaaaccaga aacctcaaaa gacgaaaagc agggggtgag
     6361 cgcgatgaca agccagcatc ctttactgaa aattgcactt aatggcgatg gacttctttc
     6421 gtggccaaat tcacccggac agactatgcg ctgcccaacg tgtatgcgag atgttattac
     6481 gggtccggat ggtgatttaa tacatcaaat ggaagagggc gctaacccct gcacgccatc
     6541 tgttgatgtt gtcatttcaa aggcgatcat tgaactactc agcacgggtg agcgcctcta
     6601 tgtcaaccca gttaagaacg cgaatagaac actggcccca tcgtttatct ttctgcataa
     6661 agacaagcaa cttcgaccat tcataaatac ggaatatcaa ccagcaggtg ccacctggag
     6721 aagtgataaa gggaacaggc ttggtttttt ctatatggat gcgcgagcgt ctaaaattaa
     6781 taaggaccag ttcgatttta ttgctgtaat agacccatct acttttcaag cagaatttaa
     6841 gaccttatgg tccatgtgtg aattcgataa cccccttgcc gcgttacagc acttattggt
     6901 ttcagagaat atgtcttcag tctgggtgaa atggcctgtt aaaagcctac aaccgaggaa
     6961 agaagcagtg aaggccgatt actggtatga ctattattca cctcactacc cagcgcagca
     7021 ggcagcttgc acagcaacag tgattggact ggaaggaaat gtatccgggg aaactattta
     7081 catgctccag atcgctgtag accggtcgat agttgagtac cagcttgtaa aatcgctagg
     7141 ggttatattg ctattggata atacgggaaa tttggtatcg gagacgcgcc cacactttga
     7201 tataatcatc aaagcttgca tctcgggtgt cagagacttt gtacgtcaaa atcctcgaat
     7261 gcgaagtgtg gagcatcaga ttaccagtgc aatatgagga aatggatcag cgactaaatc
     7321 tgttgctcgc cgtaaataca agtgcgcacc ataaaataaa aacactttat aaatcaatga
     7381 ctttgtcaaa gctcagtgaa aatgtctaat cactggctca ttataaatgt ccatatgttg
     7441 aaaaggccgc tgcgaaaatc cctcccgcag cggcctcttt agcataacgt cattatgcga
     7501 accatcactt aatgttcttc tcgcatttta agagaaatag taaatttgta gcagtaagat
     7561 gaagcatata gcttgccaat tcttccggaa cctctttaat atcagctcct tgcccatgac
     7621 ctgcattttt gtttctgatg gttggaattc ctgtttccag cattgttttc agacttgtga
     7681 actggctttg taaatatgcc ggaattaaat tttgacttaa acagctattt attagt
//
LOCUS       DAPHBJ010000204         7678 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.206, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000204 DAPHBJ010000000
VERSION     DAPHBJ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.206"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..1175
                     /locus_tag="RHZ76_003881"
                     /pseudo
     CDS             1..1175
                     /locus_tag="RHZ76_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
     gene            1541..2608
                     /locus_tag="RHZ76_003882"
     CDS             1541..2608
                     /locus_tag="RHZ76_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV2814435.1"
                     /translation="MDKSKRHLAWWVVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2605..5340
                     /gene="rbbA"
                     /locus_tag="RHZ76_003883"
     CDS             2605..5340
                     /gene="rbbA"
                     /locus_tag="RHZ76_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HDV2814436.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDILPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLIIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5340..6464
                     /locus_tag="RHZ76_003884"
     CDS             5340..6464
                     /locus_tag="RHZ76_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2814437.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDTMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(6629..7678)
                     /locus_tag="RHZ76_003885"
                     /pseudo
     CDS             complement(6629..7678)
                     /locus_tag="RHZ76_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         1830 a         1905 c         2019 g         1924 t
ORIGIN      
        1 ctgagtcgta caaacccatc tggaactgga aaatatcgca atacaatgta tttgatatta
       61 aattcgaaac aatgataaaa ccccagttcg ctgacagaac ctatttctcc aattgttcac
      121 ctgttgatcc cacaagtacc aggccgacct attttggcga taccgatggc tcagtcggtg
      181 cagttttgta cgcgcttttt gcaacgggac atttgggaat catggctgaa ggagaaaatt
      241 tccttagcca gttgcttaat atagaagatg aagttctcaa cgtattgcta agggagaact
      301 ttaatgagca acttgacact aatgttaata taataatcag tatcctgaac agaagagaca
      361 ttgttttaga aagtcttcag ccttatttgg ttattaataa agacgcagtc acgccatgta
      421 cgtttctggg cgaccagact ggcgatcgat ttagtaatat ctgtggcgat aaatttatca
      481 tcgatttatt aaaacgcata atgagtatca atgaaaacgt tcatgtatta gctggaaatc
      541 atgaaacgaa ttgcaatggt aactatatgc agaacttcac gcgcatgaaa ccacttgatg
      601 aggataccta ctctgggata aaagattatc ctgtatgttt ctatgacccc aaatacaaaa
      661 taatggcaaa tcatcatggg ataacttttg atgatcagcg aaaacgttac attataggac
      721 cgataacagt ctctagtgat gaaatgacca atgctctcta accggtcgaa ttagcggcaa
      781 ttatcaataa aaagcaccat gctgtaatta atggcaaaaa gtttaaaaat agccgggcta
      841 tctcttgtcg atcctttaat cgttattttt ctgtttcgac agattacaga cctaaacttg
      901 aagcactatt agcatgctca caaatgttag gtataaatca agttgttgca cataacggta
      961 atggtggaag agaatgtata ggtgaaacgg ggacagtttt aggtcttaac gcccgtgaca
     1021 gtaaacatgc tggaagaatg ttcagcatgc ataattgcca aattaaccct ggtgcagggc
     1081 ctgagataac cactccctgg aagtcttacc agcatgaaaa aaacagaaat ggacttatgc
     1141 ctttgatcag aaggcgcaca atgttacagc tctgaaccat agacaacatg aatattaatg
     1201 ttactatctg gttgagactg ttgataaaac gtaaaaagga atgctctgtt cctgcaactg
     1261 aacaggcgtg aaacgctatc caacaggatg gataccgttt tgtcagccag ttttatagat
     1321 cacacttatg agtatgcgtg tttcatttac ggccaggaat gctgatgatg acaacattac
     1381 tacccgtttt cactaagcct tccccgttgg cgctcaatgc tctgcgcgct ggtcgtattt
     1441 gtcgtttcct tcttatcccg gatggaagaa tccgctgaac gtcgttcggc ggcagttgta
     1501 acacgcgtcg tttgcttaca gggattatgg cgggtcgatt atggataaga gtaagcgcca
     1561 tctggcgtgg tgggttgtcg ggttactggc ggtggcggct atcgtggcgt ggtggctgtt
     1621 gcgcccggca ggtgtgccgg aaggctttgc cgtcagcaat gggcgcattg aagcgacgga
     1681 agtggatatc gccagcaaaa ttgccgggcg tatcgacacc attctggtga aagaaggcca
     1741 gtttgttcgc gaaggcgaag tgctggcgaa gatggatact cgcgtgttgc aggaacagcg
     1801 actggaagcc atcgcgcaaa tcaaagaagc acaaagtgcc gttgctgccg cgcaggcttt
     1861 gctggagcaa cggcaaagcg aaactcgtgc cgcgcagtcg ctggttaatc aacgccaggc
     1921 cgaactggac tccgtggcta aacgtcatac gcgttcccgc tcgctcgccc aacgaggggc
     1981 tatttctgcg caacagctgg atgacgatcg cgccgccgct gagagcgccc gagctgcgct
     2041 ggaatcggcg aaagctcagg tatcggcttc taaagcggct atagaagcgg cacgcaccaa
     2101 tatcattcag gcgcaaactc gcgtcgaagc cgcacaagcc actgaacggc gcattgccgc
     2161 agatatcgat gacagcgaac tgaaagcccc gcgtgatgga cgcgtacaat atcgcgttgc
     2221 cgagccaggg gaagtgctgg cggcaggcgg tcgggtgctg aatatggtcg atctcagcga
     2281 cgtctatatg actttcttcc tgccaaccga acaggcgggc acgctgaaac ttggcggtga
     2341 agcccggctg atcctcgatg ccgcgccaga tctgcgtatt ccggcgacca tcagttttgt
     2401 cgccagtgtc gcccagttca cgccaaaaac cgtcgaaacc agcgatgaac ggctgaaact
     2461 gatgttccgc gtcaaagcgc gtatcccacc ggaattactc cagcagcatc tggaatatgt
     2521 caaaaccggt ttaccgggcg tagcgtgggt gcgggtgaat gaagaacttc cgtggcctga
     2581 cgacctcgtg gtgaggttgc cgcaatgacg catctggaac tggttcccgt cccgcctgtc
     2641 gcgcaactgg cgggcgtgag ccagcattat ggaaaaaccg ttgcgctgaa caatatcact
     2701 ctcgatattc cggcccgttg catggtcggg ctgattggtc cggatggcgt cgggaagtcg
     2761 agtttattgt cactgatttc cggtgcccgc gtcattgagc agggcaatgt ggtggtgctg
     2821 ggtggcgata tgcacgaccc gaagcatcgc cgagacgtct gcccgcgcat cgcctggatg
     2881 ccgcaggggc tgggcaaaaa cctctaccac accttgtcgg tgtatgaaaa cgtcgatttt
     2941 ttcgctcgcc tgttcggtca cgacaaagcg gagcgggaag tgcgaattaa tgagctgctg
     3001 accagcacgg ggttagcacc gtttcgcgat cgtccggcag ggaaactctc cggcgggatg
     3061 aagcaaaaac ttgggctgtg ctgcgcgtta atccacgacc cggaactgtt gatccttgat
     3121 gagccaacaa cgggggttga cccgctctcc cgctcccagt tctgggatct gatcgacagt
     3181 attcgccagc ggcagagcaa tatgagcgtg ctggtcgcca ccgcctatat ggaagaggcc
     3241 gaacgcttcg actggctggt agcgatgaat gccggagaag tgctggcaac tggcagcgcc
     3301 gaagagctac ggcagcaaac gcaaagcgct acgctggaag aagcatttat aaatctgtta
     3361 ccgcaagcgc aacgccaggc gcatcaggcg gtagtgatcc caccgtatca acctgaaaac
     3421 gcagagattg ccatcgaagc gcgcgatctg accatgcgtt ttggttcctt cgttgccgtt
     3481 gatcacgtta atttccgcat tccacgcggg gagatttttg gttttcttgg ttcgaacggc
     3541 tgcggtaaat ccaccaccat gaaaatgctc accggactgc tgcccgccag cgaaggtgag
     3601 gcgtggctgt tcgggcaacc ggttgatcca aaagatatcg atacccgccg tcgggtgggc
     3661 tatatgtcgc aggcgttttc gctctataac gaactcaccg tgcggcaaaa ccttgagtta
     3721 catgcccgtt tgtttcacat cccggaagcg gaaattcccg caagagtggc tgaaatgagc
     3781 gagcgtttta agctcaacga cgttgaagat attctgccgg agtcattgcc gctcggcatt
     3841 cgccagcggc tttcgctggc ggtggcggtg attcatcgcc cggagatgtt aatcctcgat
     3901 gagcctactt ctggtgtcga tccggtggcg agggatatgt tctggcagtt gatggtcgat
     3961 ctctcgcgcc aggacaaagt gactatcttc atctccaccc actttatgaa cgaagcggaa
     4021 cgttgcgacc gcatctcact gatgcacgcc ggaaaagtgc ttgccagcgg tacaccgcag
     4081 gaactggttg agaaacgcgg agccgccagt ctggaagagg catttatcgc ctatttgcag
     4141 gaagcggcag ggcagagcaa cgaagccgaa gcgccgcccg tggtacacga caccacccac
     4201 gcgccgcgtc agggatttag cctgcgccgt ctgtttagct acagccgccg cgaagcgctg
     4261 gaactgcgac gcgatccagt acgttcgacg ctggcgctga tgggaacggt gatcctgatg
     4321 ctgataatgg gttacggcat cagtatggat gtggaaaacc tgcgctttgc ggtgctcgac
     4381 cgcgaccaga ccgtcagtag ccaggcgtgg acactcaacc tctccggttc ccgttacttt
     4441 atcgaacagc cgccgctcac cagttatgac gagcttgatc gtcggatgcg tgcgggcgat
     4501 atcactgtgg cgattgagat cccgcccaat ttcgggcgcg atatcgcgcg tggtacgcct
     4561 gtggaactcg gcgtctggat cgacggagcg atgcccagtc gtgccgaaac ggtaaaaggt
     4621 tacgtgcagg caatgcacca gagttggctt caggatgtgg cgagccgaca atcgacaccc
     4681 gccagccaaa gcgggctgat gaacattgag acgcgttatc gctataaccc ggacgtaaaa
     4741 agcctgccag cgattgttcc ggcggtgatc ccgcttctgc tgatgatgat cccgtcaatg
     4801 ctaagcgccc ttagcgtggt gcgggaaaaa gagcttgggt cgattatcaa cctttacgtg
     4861 acccccacca cgcgtagtga atttttgctt ggtaaacagt tgccatacat cgcgctgggg
     4921 atgctgaact ttttcctgct ctgcggcctg tcggtgtttg tgtttggcgt accgcataaa
     4981 ggcagtttcc tgacgctcac cctggcggcg ctgctgtata tcatcattgc caccggaatg
     5041 gggctgctga tctccacctt tatgaaaagc cagattgccg ccattttcgg aacggcgatt
     5101 atcacgttga tcccggcgac acagttttcc gggatgatcg atccggtagc ttcgctggaa
     5161 gggcctggac gttggatcgg cgaggtttac ccgaccagtc attttctgat tatcgcccgc
     5221 gggacgttct cgaaagcgct ggatctgact gatttgtggc aactttttat cccgttgctg
     5281 atagccatcc cgctggtgat gggcttaagt atcctgctgc tgaaaaaaca ggagggatga
     5341 tgcgccattt acgcaatatt tttaatctgg gtatcaaaga gttgcgcagt ctgctcggtg
     5401 ataaagcgat gctgacgctg attgtcttct cgtttacggt gtcggtgtat tcgtcagcga
     5461 ccgttacgcc aggatcgttg aacctcgcgc cgatcgccat tgccgatatg gatcaatcgc
     5521 agttatcgaa ccggatcgtt aacagcttct atcgtccgtg gtttttgcca ccggagatga
     5581 tcaccgccga tgagatggat gctggactgg acgccggacg ctataccttc gcgataaata
     5641 ttccgcctaa ttttcagcgt gatgtcctcg ccggacgcca gccggatatt caggtgaacg
     5701 tcgatgccac gcgcatgagc caggcattta ccggcaatgg gtatatccag aatattatca
     5761 acggtgaagt gaacagcttt gtcgcgcgct accgtgataa cagcgaaccg ttggtatcgc
     5821 tggaaacccg gatgcgcttt aacccgaacc ttgatcccgc gtggtttggc ggggtgatgg
     5881 cgatcatcaa caacattacc atgctggcga ttgtattgac cggatcggcg ctgatccgcg
     5941 agcgtgaaca cggcacggtg gaacatttac tggtgatgcc gataacgccg tttgagatca
     6001 tgatggcgaa gatctggtcg atggggctgg tggtgctggt ggtatcggga ttatcgctgg
     6061 tgctgatggt gaaaggtgta ctgggcgtac cgattgaagg ctcgatcccg ctgtttatgc
     6121 tgggcgtggc gctcagtctg tttgccacca cgtcaatcgg catttttatg gggacgatag
     6181 cgcgttcaat gccgcaactg gggctgctgg tgattctggt gctgctgccg ctgcaaatgc
     6241 tttccggtgg atccacgccg cgcgaaagta tgccgcagat ggtgcaggac actatgctga
     6301 ccatgccgac gacacacttt gttagcctcg cgcaggccat cctctaccgg ggtgccggat
     6361 tcgaaatcgt ctggccgcag ttcctgacgc tgatggcaat tggcggcgcg tttttcacca
     6421 ttgcactgct gcgattcagg aagacgattg ggacaatggc gtaaatacaa ttgcccgatg
     6481 cgtaagctta tagggactac gcatcggtgc aatttattga attttagttg tttttaggcc
     6541 ggataaggcg tttatgaact gacccccaaa acctggacag tttcagttaa ccggcttttt
     6601 gatactcagt cctgtattca gggcaagatt acgaaagccc gctccccgca aggactgacg
     6661 ccagatagtt tctgtccatg gctgcttttc gcatcttacg tcttaaccct gccttgaata
     6721 ccttatcatt cgtcaaaata ttaatagcaa tgtgccgtat ccctgaaaat aattctgctg
     6781 catttcctct tcttattttg cagtcgtctt cattcattac cacgtccaga cgccagtgca
     6841 gcttattctc cacgtgccag tggtttcgga ttgctgtggc gaacttctct gcggttaaat
     6901 cagcagaact gatataatat ctgaccgtca tttctggctc tttcttttgt tctgctatta
     6961 tggaccgaaa ggagactgcc acgcataatt tcttcagccc tttccattca aacgtgaaat
     7021 caataagttc atcagggaca tcgcaaacaa tatgaagacg gatttcttct ctgccgtgac
     7081 tcttttcact cattgcgtaa ctgtcatgct ctggattatt taattctttc agcggaaatt
     7141 tttcctcaaa ggctttattt agccgcccct ggtttccttt taccgcgaat aaataattac
     7201 ctccctgttt ttgtatcttt tctgcaatat ctttctggca gcccatcgca tcagttgtga
     7261 tgatttttcc tttaatatcc atcatgttaa gaagttcagg aatggctgta atctcattgg
     7321 atttctcatc cgtcttgatc tgtccgagga ccagactatg cattgttgag aacgcactaa
     7381 tgacatgaat cgctcccctg cgacgactct tgtcataaga gtgccggagc gtttttccat
     7441 caattgcaat gacgtcttta tcatctgaag aatggcagtc acgcatccag ttaataaagc
     7501 actcgtgaaa ttttgcagga ctgatacagg atacaactct ggcaatgata tcgtgaacag
     7561 gaataccatt ttcaaaatca ccatatcact tcaaaacatc gggatgtgtt tccccaaaat
     7621 cctctatatc ttcccaacct tctgcaccag aaataacggc acaaatagtc aacagtag
//
LOCUS       DAPHBJ010000205         7559 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.207, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000205 DAPHBJ010000000
VERSION     DAPHBJ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.207"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(80..544)
                     /locus_tag="RHZ76_003886"
     CDS             complement(80..544)
                     /locus_tag="RHZ76_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HDV2814438.1"
                     /translation="MRFCLILITALLLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            complement(622..1371)
                     /gene="btuD"
                     /locus_tag="RHZ76_003887"
     CDS             complement(622..1371)
                     /gene="btuD"
                     /locus_tag="RHZ76_003887"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HDV2814439.1"
                     /translation="MSIVMQLQDVAESTRLGPFSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            complement(1371..1922)
                     /gene="btuE"
                     /locus_tag="RHZ76_003888"
                     /pseudo
     CDS             complement(1371..1922)
                     /gene="btuE"
                     /locus_tag="RHZ76_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
     gene            complement(1985..2965)
                     /gene="btuC"
                     /locus_tag="RHZ76_003889"
     CDS             complement(1985..2965)
                     /gene="btuC"
                     /locus_tag="RHZ76_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HDV2814440.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            complement(3066..3365)
                     /gene="ihfA"
                     /locus_tag="RHZ76_003890"
     CDS             complement(3066..3365)
                     /gene="ihfA"
                     /locus_tag="RHZ76_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HDV2814441.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(3370..5757)
                     /gene="pheT"
                     /locus_tag="RHZ76_003891"
     CDS             complement(3370..5757)
                     /gene="pheT"
                     /locus_tag="RHZ76_003891"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDV2814442.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVQPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVMYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            complement(5772..6755)
                     /gene="pheS"
                     /locus_tag="RHZ76_003892"
     CDS             complement(5772..6755)
                     /gene="pheS"
                     /locus_tag="RHZ76_003892"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDV2814443.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     misc_feature    complement(6965..7089)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(7039..7083)
                     /gene="pheM"
                     /locus_tag="RHZ76_003893"
     CDS             complement(7039..7083)
                     /gene="pheM"
                     /locus_tag="RHZ76_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HDV2814444.1"
                     /translation="MNAAIFRFFFYFST"
     gene            complement(7206..>7559)
                     /gene="rplT"
                     /locus_tag="RHZ76_003894"
     CDS             complement(7206..>7559)
                     /gene="rplT"
                     /locus_tag="RHZ76_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDV2814445.1"
                     /translation="ARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQY
                     AYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFDK
                     VAFTALVEKAKAALA"
BASE COUNT         1805 a         2107 c         1844 g         1803 t
ORIGIN      
        1 gaatttttgt ctgcaaaata ctactgtatt caggagtaaa tggacggcga tgtatcatca
       61 ccgtccaatt ttcgacatac tagattcgtc tcgcctgcca gaaatttttc tgccaataga
      121 cattatcaag tgaggaacgc atcactccct tgctggtaga ggcgtggata aattggttgt
      181 tggtatcgta aatacctaca tgcaaaccat tttgtccgga acccgttttg aaaaagacca
      241 ggtcaccagg cagcaactcg tctttatcaa tttgcgtgcc gatagaggct tgttgtttgg
      301 tttctcgggg cagttgcaaa tcaaaacgat cgcgcatcgt cacaaccaca aatcccgaac
      361 agtccacacc gcgccgcgtc atgccaccat aacgatacgg cgtgccatgc cagctttgta
      421 gctggtcgtt caaaccggca ataacggtaa tcgaatcaga aagtctggca tttggcggcg
      481 gtgctttatg gtggctacac ccggccagaa gcagtgctgt gatcaaaata aggcagaaac
      541 gcattccgta cggttcctct gttttttatt cttgcattaa tttagcgtcg taattacccg
      601 attttcaaga tactaatgaa atcagatggt cgaaatcagc attctgtgac cttcgatatc
      661 cagacggcga aaattcatcc cataggcctg cgccagattt ggcggcgtga gcacctcttc
      721 cctgcgtcca ctggccagca tttttccacc ttttagcaac cacgcccgat gcgcatgacg
      781 caatgtgtgg ttgagatcgt gactgctcat cacaatcgcc agtccttgct gacacagcgc
      841 gctcagaatt ttgtctaacg cactttgttg cgcaacatca agactgttca tcggctcatc
      901 aagaagcagc aattggcctg cgggattggc ttgtggtgtg atttgcagca ccaccgcagc
      961 aagacgtacg cgttgccatt caccgccgga aagttgattg gtgctacgtc cgagtttgtc
     1021 atcaagagcc agcgcccctg cgacatcatt cagtagttcg gtacgcgttt tatcgtgctg
     1081 atgcagtgtc aggtagtgcc agaccggcat tgcaaacggc ggcgtctgct gttgtgaaag
     1141 gtaggcgcga tgcagcgcga gttttgttgc ggaccatgct tccagtggtt gccccgcgaa
     1201 ctgaatgctt cccttaccgc tggtcattcc ggccattcgt gcaagcaagg tactcttacc
     1261 cgcgccattc ggccccacca ggtgcaggat ctccccagcc cgaacctcgc cagaaaacgg
     1321 ccccaggcgg gtagattccg caacatcttg taactgcatc acaatagaca ttattttgcc
     1381 aacgccagtt taatgctttc catcacaatg ggatcttccg gcgtcatatc cggggaaaaa
     1441 cgctggatga cttttccgtc catgccaacc aggaattttt caaaattcca taaaatatca
     1501 tccgggtaca gcggtgcacg gcctttgctg accataaggg catagaatcc gctctcttcc
     1561 ggcgcgactg cggtcggcgc tgcggcaatc aatttttgat acagcggatg gcgtccttcg
     1621 ccattaactt caatcttact gaacatcggg aacgtcaccc cccatgtggt ggtacagtaa
     1681 gttttaatct attcatcgct gcccggttct tgttccagaa actggttgca cgggaatccc
     1741 agcaccacaa aacctcgatc ggcccaggct ttctgaatat tctccaactg ctcatattgc
     1801 ggcgttaagc cacactttga ggcgacattg acaatcaaca gcacattacc ggcgtacttc
     1861 tccagcgtgg tcacttcacc gtcgatatct ttcactacgg tcgtcagaat ggaatcttgc
     1921 atcgtttctc ctgggtgtgg tcagtaaaaa tcttagcttt taatcataga ccgtcttttt
     1981 gcggctaacg tcttgctttt aacaataacc agataaacac cggcgcaccc aacgttgcgg
     2041 tgaccacgcc aataggcagc tctgcggcag ctaatgccag gcgcgctaca atatcggcca
     2101 gcagcaatgc gctcgcccct gccagcgcgc agccgggaag taatacgcga tgatcggtta
     2161 aaccacacaa ccgaagaata tgtgggatca ccagaccaat aaagccgata gcacccgcca
     2221 gcgccacact gacgccaacc atccagccgg tcgcggccac cagcacattg cgccagaacc
     2281 acaggggtaa acccagttgc cgcgccgaga tctcgccaag tgctaacata ttcatcggcc
     2341 tggactgaca acagatccac aacaacacgg ggatcaatgc cagcatcagc cagctttgcc
     2401 gccagtctac gccgccaaaa ccgcccatca tccagtacat cagctgacgt aaatcaacag
     2461 aggtggaaaa gtagatagcc cacgtcatta gtgcgctaca gataatccct aatgcaacgc
     2521 cagccagcaa taaccgactg gtcgaaagat gacgacgggc gaaacgtaag agtattaaag
     2581 tgatgataag cgcgccagca atcgcacaca gccctagcgc ccagttgggg agttgccctt
     2641 gcccaagcaa taccgcggcg ataagcccca cgcctgcgcc gttagagacg ccaagtagtc
     2701 caggttctgc cagaggattt tcaaacaacg cctgcattac agcgccggat atagccagcg
     2761 ccgcaccaac cagcaataca gccagcgtac gtggcaggcg aatttgccag acgaacagtt
     2821 cgccacgagg agtaaaccag tcacctggcg agatccattg ttcaccggcg caaaggctta
     2881 agagaagcgc cagcagcatc aaaactgaca ggcataataa ccagcgaata ttttgtcgct
     2941 gttgttggcg ggcaagtgtc agcatggtat ccgttctgct gaagtgtcat ggcgttgatt
     3001 ttacggtgac tcttcgacag tgaaaagaaa aaaggccgca gagcggcctt tttagttaga
     3061 tcagattact cgtctttggg cgaagcgttt tcgacccggc tttttaactt ctgcccgggt
     3121 ctgaaggtca ccacgcgccg tgctgtaatg ggaatatcct cgcccgtttt cgggttacgt
     3181 cccgggcgtt gattcttatc acgcagatcg aagttaccaa aaccagagag tttcacctgt
     3241 tcgccgtttt ccagagcgcg acggatctct tcgaaaaaca gttcaaccag ttctttggca
     3301 tcccgcttgc taagcccaag cttatcaaac agatattctg acatttcagc ttttgtaagc
     3361 gccataggtt caatccctca atgatgcctg gaatcgctct tttaatgcct ctacacattt
     3421 ggcgacggta gcggcaatct cctcttcttc gagtgtacgg ctggtatctt gcaggatcag
     3481 gcttatggcg aggctcttat acccctccgc aacaccctta ccgcggtaca cgtcaaataa
     3541 gtttacgcca actacctgat ttacgccaac tttcttacat tcggataaaa tatccgctgc
     3601 gggaacgttt tctgcgacca ccaccgcgat gtcacgacgg ttcgccggga agcgagaaat
     3661 ctcgcgcgcc tgaggcacca cgcggtctgc gagcttgttc cactccagtt cgaacaccag
     3721 agtgcgaccg ttaagatcca gtttacgttc cagttcagga tgaacaaccc caacaaaacc
     3781 aatacgttca cctttcagat aaatcgctgc ggattgcccc ggatgcagtg ccggattcgc
     3841 ttctgcacgg aactcaacct cattcagttt accggtcagg tcgagaacgg attcaagatc
     3901 gcctttcaaa tcatagaaat caacggtctc ttttgccagg ttccagtgct cttcgtaacg
     3961 gttaccgcaa atcacaccgg ctaacatcag atcctgacga atgcccaacg gtgcctgagt
     4021 atctggtacg aaacgcagac cgctttcgaa aatgcgcaca cggttctgct gacggttctg
     4081 gttgtacatc acggttgcca gcaggccagt ccacagagaa agacgcattg ctgacatttc
     4141 aacagagatc gggcttggca gcagtaaggc ttcaacgcct ggatggatca tctgctgcac
     4201 tttcggatca acgaagctgt aggtgatcac ttcctgatag cctttgtcgt tgagcagcgt
     4261 tttcacgcgc ttgagcgaca ggtcagcttc acggtgagta cccataatca ggcttgcctg
     4321 taccggctca tccgggatgt tgttgtagcc gtaaacacgc gcgacttctt caaccagatc
     4381 ttcttcaatc tccatatcga aacgccagct cggcgcaact gcctgccatt cgtctttgcc
     4441 ttcggtcact tcgcagccga gacgacgcag aatgtcagtt acctgctcat ccgcaatatg
     4501 atggccgatc aggcgatcca gtttgctacg acgtagagtg atggttgcac gcttcggcag
     4561 cgttgcttcg ttggtgatat caattaccgg gccagcctca ccaccgcaga tgtcgatcag
     4621 cagacgggtc gcacgttcca tcgctttgtg ctgcagtgcc ggatcaacgc cacgctcata
     4681 acggtgagac gcatcggtat gcaggccatg acgacgagca cgaccggtga tagacagcgg
     4741 gctaaagaac gcgcattcca gcagcacgtt ttgtgtttcg tcattcacgc cagagtgttc
     4801 gccaccgaag atgccgccca tcgccagcgc cttgttgtgg tcggcgatga ccagagtgtc
     4861 agcattcagc ttcgcttcag taccgtcgag cagcaccagc gtttcgccct ctttcgccat
     4921 ccgcaccaca atgccgcctt caatgcgatc tttatcgaaa gcgtgcatcg gctggcccag
     4981 ttcgagcagc acatagttgg tgacgtcaac aactgcatcg atagaacgga tcccgcaacg
     5041 acgcagtttt tctttcatcc acagcggagt tggcgcttta acgttaatgc cttttaccac
     5101 acggccaaga taacgcgggc aggcttccgg cgcttcgact gtaatcggca gcgtgtcgtc
     5161 gatggtcgca ccaaccggaa cgatttccgg ttgaaccagc ggcagctggt tcagcacggc
     5221 aacgtcacgc gcaacaccaa tgatgcctaa gcagtcggca cggtttggcg tcacgctgat
     5281 ttcgatggtg ttgtcatcaa gtttcaggta ttcacggata tcggtgccaa tcggcgcatc
     5341 cgcaggcagt tcgataatgc cgctgtgatc gtcagaaatg cccagttcag agaaggagca
     5401 cagcatccct tcagacggtt cgccacgcag tttcgccgct ttaattttga aatcacccgg
     5461 cagaacagca ccaatggtcg ctaccgccac acgcaggccc tgacggcagt ttggcgcacc
     5521 gcagacgatg tccagcaggc gatcgccgcc gacattcact tttgtcacac gcagtttgtc
     5581 agcgttcgga tgctgcgcac actcaaccac ttcaccaacg accacgccgt ggaagctgcc
     5641 ggcaaccggt tctacaccgt caacttccag gcccgccata gtgatttgat ttgccagcgc
     5701 atcgctatca atcgccgggt tcacccattc gcgtaaccac agttcactga atttcataat
     5761 ctattcctgc cttatttaaa ctgtttgagg aaacgcagat cgttttcgaa gaatgaacgc
     5821 aggtcggtga cgccgtaacg caacatagtc agacgctcca tccccatccc gaaggcgaaa
     5881 ccagagtaaa cttccgggtc gatgccaacg ttacgcaaca cgttcggatg caccatcccg
     5941 cagcccagca cttccagcca tttaccgttt ttacccatga cgtccacttc tgcagaaggt
     6001 tcggtaaacg ggaagtagga aggacggaag cgaatctgca aatcttcctc aaagaagtta
     6061 cgcaggaagt cgtgcagcgt gcctttcagg ttggtaaagc tgatgttggt atcaacaatc
     6121 agaccttcca tctgatggaa catcggcgtg tgagtctggt cgtagtcgtt acgataaaca
     6181 cggccaggcg cgatgatacg aatcggtggc tgctgggctt tcatggtgcg gatctgtacg
     6241 ccagaggtct gggtacgcag caggcgggta gtgtcaaacc agaaagtgtc gtggtcagcg
     6301 cgcgccgggt ggtgaccagg aatgttcaga gcatcgaagt tatgataatc gtcttcgatt
     6361 tccggcccgg ttgccacggt aaagccaagc tcaccgaaga aactttcgat acggtcgatg
     6421 gtacgggtaa ccggatgcag accgccgttt tcaatgcgac gacctggcag agagacatca
     6481 atcgtttccg ccgccagacg cgcattcagt gcagcgcttt ccagttccgc tttacgcgca
     6541 ttcagcgcct gctgaacctg ctctttcgct tcgttgataa ccgcaccagc tgccggacgc
     6601 tcttctggcg gcagctcacg cagggtcgtc atctgaaggg ttaagtgccc ttttttaccc
     6661 aaatattcga cgcgcacatt atctaacgcg gcaacatctg acgcctggct aatggccgcc
     6721 ttcgcactgg caaccagttc tgcgagatgt gacatggttt tcctcattgt gtcagtggtg
     6781 acactggttc gttggactta gagcctatcc catcaggcta ttttacttgc cattttggtc
     6841 ccgggctgtg ctcgaaattc tcacgtactt aaatacgctc cggtttctcc gcgctggccg
     6901 tgtccagtct ggctgcgaca attacacctg atgagacagg ctttttattt ttcaaaacgc
     6961 gcatacaaaa aaagcctcca ctgggaggct ttcaggcgct gttttccgtt tctcttctca
     7021 cgcgctagcc tcctggattc aggtgctaaa gtaaaaaaag aagcggaaaa tagcagcatt
     7081 cattgcttgc gttacctttt ggtactcttc aaaagacctt tattgaaaag gctacggcga
     7141 taaaagtcaa tgttttgatg gcgttgaaac gaaaagaggg agactagctc cctctttcaa
     7201 ctggcttatg ccagagctgc tttcgctttt tcaaccagag cggtgaacgc tactttgtcg
     7261 aatactgcga tatcagccag gatcttacgg tcgatttcaa cagaggcttt tttcaggcca
     7321 ttgatgaatt tgctgtaaga aataccgttc tgacgtgctg ctgcgttgat acgcgcaatc
     7381 cacagttgac ggaactgacg cttacgttga cgacggtcac ggtaagcata ctgaccagct
     7441 ttgataacag cctggaaggc aacgcggtat acgcgagaac gcgcaccgta gtagccttta
     7501 gcttgtttca aaattttctt gtgacgtgcg cgtgcaataa caccacgttt tacgcgagc
//
LOCUS       DAPHBJ010000206         7513 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.208, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000206 DAPHBJ010000000
VERSION     DAPHBJ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.208"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..396
                     /gene="yiaM"
                     /locus_tag="RHZ76_003895"
     CDS             <1..396
                     /gene="yiaM"
                     /locus_tag="RHZ76_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HDV2814446.1"
                     /translation="RYGFQTSILSVDELSRYLFVWLTFIGAIVAFMDNAHVQVTFLVE
                     KLSPAWQRRVALVTHSLILFICGALAWGATLKTIQDWSDYSPILGLPIGLMYAACLPT
                     SLVIAFFELRHLYQLITRSNSLTSPPQGV"
     gene            399..1676
                     /gene="yiaN"
                     /locus_tag="RHZ76_003896"
     CDS             399..1676
                     /gene="yiaN"
                     /locus_tag="RHZ76_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HDV2814447.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLALYSLLVVFVFIPDLIILPLKWIN"
     gene            1689..2675
                     /gene="yiaO"
                     /locus_tag="RHZ76_003897"
     CDS             1689..2675
                     /gene="yiaO"
                     /locus_tag="RHZ76_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HDV2814448.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKFLSLTHHAYSPLLVVINKAKFDGLSPEFQQALVSSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGNQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            2679..4175
                     /gene="lyxK"
                     /locus_tag="RHZ76_003898"
     CDS             2679..4175
                     /gene="lyxK"
                     /locus_tag="RHZ76_003898"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HDV2814449.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRALLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAALTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYHNFSE
                     AQRDLRHPVRTLLPDMTAHQLYQKKYQRYQHLIAALQGFHARIKEHTL"
     gene            4172..4834
                     /gene="ulaD"
                     /locus_tag="RHZ76_003899"
     CDS             4172..4834
                     /gene="ulaD"
                     /locus_tag="RHZ76_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDV2814450.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTLLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPAQVAGDFHAQIDAIWGGA
                     RA"
     gene            4827..5687
                     /locus_tag="RHZ76_003900"
     CDS             4827..5687
                     /locus_tag="RHZ76_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDV2814451.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTG
                     QSPGQFRDVPFGEGCVDFVGIFKTLHKLNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            5681..6376
                     /gene="araD"
                     /locus_tag="RHZ76_003901"
     CDS             5681..6376
                     /gene="araD"
                     /locus_tag="RHZ76_003901"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDV2814452.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(6408..6509)
                     /locus_tag="RHZ76_003902"
                     /pseudo
     CDS             complement(6408..6509)
                     /locus_tag="RHZ76_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            6513..6701
                     /locus_tag="RHZ76_003903"
                     /pseudo
     CDS             6513..6701
                     /locus_tag="RHZ76_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6723..7463)
                     /locus_tag="RHZ76_003904"
     CDS             complement(6723..7463)
                     /locus_tag="RHZ76_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HDV2814453.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
BASE COUNT         1707 a         2011 c         2025 g         1770 t
ORIGIN      
        1 agatatggtt ttcagacaag cattttatct gttgatgaat tgtcacgtta tttatttgtc
       61 tggttaacgt ttattggcgc gattgtagct tttatggata acgcccacgt tcaggtcacc
      121 tttctggtgg agaaactctc ccccgcatgg cagcgacgag tcgctctggt tacccattcc
      181 ttaatcttat ttatttgtgg cgcactggcc tggggcgcaa cgctaaaaac aattcaggac
      241 tggagcgatt attcaccgat cctcggttta cccatcggcc tgatgtatgc cgcttgtctg
      301 cccaccagcc tcgttatcgc attctttgaa ctgcgtcatt tatatcaact catcacgcgt
      361 agcaattctc taacgtcgcc accgcaagga gtttaatcat ggctgtgctg atttttctgg
      421 gctgtctgtt gggtggtatt gctatcggtt tgcctattgc ctgggcactg ttgttgtgcg
      481 gggcggcgtt aatgttctgg ctggacatgt ttgatgtcca gatcatggca caaacgctgg
      541 tgaacggagc cgatagcttc tccctgctgg cgattccgtt ctttgtgctg gcgggtgaaa
      601 tcatgaatgc gggcggcttg tcaaaacgca ttgttgacct gccgatgaaa ctggtggggc
      661 ataaaccagg cgggctgggc tacgtcggcg tgctggcagc tatgattatg gcgagccttt
      721 ctggttctgc cgttgcggat accgccgccg ttgccgctct actggtgccg atgatgcgca
      781 gcgccaacta tccggtcaac cgggcggcgg ggctgattgc ctctggcggc attatcgcgc
      841 caattattcc cccttccatt ccgtttatta tcttcggcgt ttccagcgga ttatccatca
      901 gtaagctgtt tatggcgggc attgcccctg gcatgatgat gggcgcaacg ctgatgctta
      961 cctggtggtg gcaggccagt cgccttaatt taccccgcca gcaaaaagca acgatgcagg
     1021 aaatctggca ctcttttgtc tccggtatct gggcgctgtt tcttccagtc atcattattg
     1081 gcgggtttcg ctccggtctg tttaccccta ccgaagccgg ggccgttgct gctttctatg
     1141 cgctgtttgt cgccacagtt atttaccgtg aaatgacctt cgccacactt tggcatgtac
     1201 tcattggcgc ggcgaaaacc acctcagtgg ttatgtttct ggtggcctcg gcacaagttt
     1261 ccgcctggct gattaccatt gctgaactgc cgatgatggt ttctgattta ctgcaaccgt
     1321 tggtcgattc accgcgtctg ctgtttatcg tcattatggt ggctatcctg attgtcggca
     1381 tggtcatgga tttaacgccg acggtattaa ttcttacccc ggtattaatg cctttagtta
     1441 aagaggcagg aatcgatccg atttatttcg gtgtcatgtt tatcattaac tgctcaatcg
     1501 gcttaattac accgcctatc ggtaatgtac ttaacgttat ttcaggggtg gcaaaactca
     1561 aattcgatga tgcagtcaga ggcgttttcc cttacgtcct ggctttatat tcattattag
     1621 tcgtatttgt ttttattccc gatctcatca tcctcccttt gaaatggatt aattaaaagg
     1681 aaaatattat gaaattacgc tctgtaacct acgcattatt cattgctggc ctggctgcat
     1741 tcagcacatc ttctctggcg gcacaatctt tacgtttcgg ttatgaaaca tcacaaaccg
     1801 actcgcaaca tattgcggcg aaaaaattca atgatttatt gcaggagaga accaaaggcg
     1861 agctgaaatt aaaactgttc ccggacagca ctctcggtaa cgcgcaggcg atgatcagcg
     1921 gcgtacgtgg cggcaccatc gatatggaaa tgtccggctc gaataacttt gccgggttat
     1981 caccagtgat gaacttgctt gatgtccctt tcctgttccg cgataccgct cacgcgcata
     2041 aaacgctcga cggcaaagtc ggtgatgatc tgaaagcctc acttgaaggt aaaggactga
     2101 aagtactggc ctactgggaa aacggctggc gcgatgtcac caactcgcgc gcaccggtta
     2161 aaacccccgc cgacctgaaa gggctgaaaa tccgcaccaa caatagcccg atgaatatcg
     2221 ccgcattcaa agtctttggc gctaacccga tcccgatgcc gtttgccgaa gtctataccg
     2281 ggctggaaac ccgcactatc gacgctcagg aacacccgat caacgtcgtc tggtcagcaa
     2341 aatttttcga agtgcagaag ttcctttctc tgacgcacca cgcctattcc ccgcttctgg
     2401 tggtgatcaa caaagcgaag tttgatggct taagtccgga gttccagcag gcgctagttt
     2461 catctgcaca agaagcgggt aactatcagc gcaaactggt tgctgaagat cagcaaaaaa
     2521 tcatcgacgg catgaaagaa gcgggcgtgg aagtcatcac cgatctcgac cgcaaagcct
     2581 ttagcgacgc actggggaat caggttcgcg acatgtttgt taaagatgtg ccgcagggag
     2641 ctgatctgct gaaagccgtg gatgaggtgc aataaaccat gacgcaatac tggctggggt
     2701 tagattgtgg cggtagctgg ctgaaagccg ggctgtatga ccgcgaaggc cgggaggcag
     2761 gcgtgcagcg cctgccgctg tgcgcattaa gcccgcagcc aggctgggca gagcgcgata
     2821 tggcagaact gtggcaatgc tgcatggctg tcattcgcgc cctgcttact cattctggtg
     2881 ttagcgggga acaaattgtc ggtatcggca tctccgcaca gggaaagggc ttgtttttgc
     2941 tggataaaaa cgacaaaccg ctcgggaatg ctattttgtc ctcggaccgc cgggcgatgg
     3001 aaatcgttcg tcgctggcag gaagatggca tcccggaaaa actctacccg ctgacccgac
     3061 aaaccttgtg gaccgggcat ccggtgtcgc tgttacgctg gctgaaagag cacgaaccag
     3121 aacgctacgc gcaaattggc tgcgtgatga tgacgcacga ctacctgcgc tggtgtttaa
     3181 ctggcgtcaa aggctgtgaa gagagcaata tttccgagtc caacctctac aacatgagtc
     3241 ttggggaata tgacccgtgc ctcaccgact ggctggggat cgctgaaatc aatcacgccc
     3301 tgccgcctgt tgtcggatct gccgaaatct gcggggagat caccgctcag acagccgccc
     3361 tgaccggtct gaaagcgggt acgcccgttg ttggcggcct gtttgatgtg gtttccaccg
     3421 cactctgcgc cgggatcgaa gacgaattta ccctcaatgc ggtgatgggg acctgggcgg
     3481 tgaccagcgg cataacccgc ggtttacgtg acggtgaagc gcatccgtat gtctatggtc
     3541 gctacgttaa cgatggtgaa tttatcgttc acgaagccag ccctacctct tccggcaacc
     3601 tcgaatggtt taccgcacag tggggagaaa tctcgtttga tgagatcaat caggccgttg
     3661 ccagcttgcc gaaggctggg ggcgatctct ttttcctgcc gttcctgtac ggcagcaacg
     3721 ccggactcga gatgaccagt ggtttctacg ggatgcaggc cattcacacc cgcgcgcacc
     3781 tgttgcaggc catctatgaa ggggtggtgt tcagccatat gacccacctc aaccgaatgc
     3841 gcgaacgttt tactgatgtt cacaccctac gcgtcactgg cggcccggcg cactccgatg
     3901 tctggatgca aatgctggcg gacgtcagcg gtctgcgtat cgagctgccg caggtggaag
     3961 aaaccggctg ctttggtgcg gcccttgccg cccgcgtcgg caccggggtt tatcacaact
     4021 tcagcgaagc ccaacgtgac ttgcgacacc cggtgcgcac cctgctgcca gatatgaccg
     4081 cccatcagct ttaccaaaaa aaatatcaac gttatcagca tctcattgcc gcacttcagg
     4141 gctttcacgc ccgcattaag gagcacacat tatgagccga ccacttctgc aactggccct
     4201 cgaccactca tcacttgaag ccgcgcagcg cgacgtgacg ctgttaaaag acagcgtcga
     4261 tatcgtcgaa gcgggcacca ttctctgttt aaacgaaggg cttggcgcgg tgaaagcctt
     4321 gcgcgaacag tgcccggaca aaatcatcgt tgctgactgg aaggtcgccg acgctggtga
     4381 aacgctcgcg caacaggcgt ttggcgcagg cgctaactgg atgaccatca tctgcgccgc
     4441 gccgctcgcg acggtagaaa aaggccacgc aatggcacaa cgctgcgggg gtgaaattca
     4501 gatagagctg ttcggtaact ggacgctgga cgacgcccgc gactggcatc gtattggcgt
     4561 gcggcaggcc atttatcatc gcggtcgtga tgcacaggcc agcgggcaac agtggggcga
     4621 agccgatctg gcacgcatga aggcgctttc agatatcggc cttgagcttt ccattactgg
     4681 cgggattacc cctgctgacc tgccgctgtt taaagatatc cgcgtgaaag cgtttattgc
     4741 cgggcgggca ctggcaggcg cggcaaatcc ggcgcaagtg gctggcgatt tccatgcgca
     4801 aatcgacgct atctggggag gtgcgcgtgc gtaatcatcc gttagggatt tatgaaaaag
     4861 cgctggcgaa agatctctcc tggccggagc ggctggtgct ggcaaaaagc tgcggttttg
     4921 attttgtcga aatgtcggtg gatgaaaccg acgaacggct ctcacgtctt gactggagcg
     4981 ccgcacaaag gacttcgctg gttgccgcga tgatcgaaac aggtgttggg atcccgtcga
     5041 tgtgcctgtc cgcacatcgt cgctttccct ttggtagccg tgacgaggca gtgcgcgaac
     5101 gggcgcggga aatcatgagt aaagcgattc gcctggcgcg cgatctcggc attcgcacca
     5161 tccagctggc aggttatgac gtctattacg aagaccacga cgaaggcacc cggcaacgtt
     5221 ttgctgaagg gctggcgtgg gcagtcgaac aggcggcggc atcacaagta atgctggcgg
     5281 tggagattat ggataccgcg tttatgaact ccatcagcaa atggaaaaaa tgggacgaga
     5341 tgctcgcctc accgtggttc accgtctacc cggacgtcgg caacctcagc gcctggggca
     5401 atgatgttcc cgccgaactg aaactgggca ttgaccgtat cgccgcgatc cacctgaaag
     5461 acacccagcc agtcaccggg caaagtcccg gacagttccg cgacgtgccg tttggcgaag
     5521 gctgcgtcga tttcgttggc atcttcaaaa cgctgcataa actgaactat cgcggttctt
     5581 tcctgattga gatgtggacc gaaaaagcca aagagccggt gctggagatt attcaggcgc
     5641 ggcgttggat tgaagcgcgt atgcaggagg ctggatttat atgttagagc aactgaaagc
     5701 cgacgtgctg gcggcgaatc tggcgcttcc cgctcaccat ctggtgacgt tcacctgggg
     5761 caatgtcagc gcggtagacg aaacgcggca atggatggta atcaaacctt ccggcgtcga
     5821 gtacgacgtg atgaccgccg acgatatggt ggtggttgag atagccagcg gtaaggtggt
     5881 ggaaggcagc aaaaaaccct cttccgatac accaacgcat ctggcgctct accgtcgcta
     5941 tgccgaaatt ggcggtattg tgcataccca ctcgcgccac gccaccatct ggtcacaggc
     6001 cgggctggat ctccccgcct ggggcaccac ccacgccgat tatttttacg gtgccatccc
     6061 ctgcacgcga cagatgaccg cagaggagat taacggcgaa tatgaatatc agaccggcga
     6121 agtgatcatt gaaaccttcg aagaacgtgg caggagtccg gcacaaatcc cggcggtgct
     6181 ggtgcattct cacggcccgt tcgcatgggg taaaaacgcc gccgatgccg tgcataacgc
     6241 cgtagtactc gaagaatgcg cctatatggg tctattctcg cgccagcttg cgccgcagct
     6301 ccctgcgatg caaaacgaac tgctggataa gcactacctg cgtaagcatg gggccaatgc
     6361 ctattacggg cagtaatccc tcacgccggg gcttcatcgc cccggcacta cgaattgata
     6421 tgttccttgc tgtaacgccg cttccacgct gctggcgtta aaccagtatg tttctgaaaa
     6481 atctgccgga aatagccaac gtcattaaac cgtattgcat ggagcaggcc gacaacgtcg
     6541 agtcactgca taacctgttg ctgcccgccg atgcgccatt tacgacattt gaaggcaagg
     6601 gattgttcag ccataagatc ctgattcaga cgccaggttc ccggtatgaa cagagcaatc
     6661 cagagcaaca acctttgtgg cattacgacg gagcaccagc cgcaccgaca tccaccggtg
     6721 aattaaaaac gccagctcac cccggtgaca aaggtaaaac tctcattacg atcaatcatc
     6781 gggctgtcgg taatctcttc cggcagctcg ctgtatcctg cgctcgccat caatacgacg
     6841 tgctctccta tcgggtattt tgccgtcagg ctgacatagg gcacccaggc atcctgcgcg
     6901 gaataacttg ccagaccgct acgacgggac tcactctctg aaatgccata gtaatagtca
     6961 ctgaaattct cgtcataata gagcacgccc agcgcaggtg tcagcgacag gcgacctatc
     7021 tgcattttgt ggaaaaccga tagctccccc acccagccgt tgctgttatc cagaacgtcc
     7081 gcagccgcag aggctttcac agttccccaa cgctcgtggt gataccaggc aacccccgcc
     7141 atagccgtac tatcacgctt atcaagctgt tgcatggcat aatcgtcatt atcggcaggg
     7201 tcaaattcca gcggcatcca ggatgcggtc aggctaagtt catttttttc actttgcgac
     7261 agaataaaac ctaacgtggt ctgacgaaca taaaaagaat caccttcata actaatcagg
     7321 ggaattgcct ttgtattttc attataaccg cgataaggcg attcattata agccgcaccg
     7381 gcaccaatgg ataattcaga agcagttgcg cttgccataa aaggcaacgc aaataacgcc
     7441 acaatattgc ggttaattaa catgttcggt attccatttt aaaataagat aaaaaagagt
     7501 cggcatagta atg
//
LOCUS       DAPHBJ010000207         7506 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.209, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000207 DAPHBJ010000000
VERSION     DAPHBJ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.209"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            15..869
                     /gene="gatY"
                     /locus_tag="RHZ76_003905"
     CDS             15..869
                     /gene="gatY"
                     /locus_tag="RHZ76_003905"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDV2814454.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVNAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLSFAQNISRVKKVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            898..2160
                     /gene="gatZ"
                     /locus_tag="RHZ76_003906"
     CDS             898..2160
                     /gene="gatZ"
                     /locus_tag="RHZ76_003906"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HDV2814455.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHISQVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYRELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            2170..2622
                     /gene="gatA"
                     /locus_tag="RHZ76_003907"
     CDS             2170..2622
                     /gene="gatA"
                     /locus_tag="RHZ76_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HDV2814456.1"
                     /translation="MTNLFVRSGISFVERSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            2653..2937
                     /gene="gatB"
                     /locus_tag="RHZ76_003908"
     CDS             2653..2937
                     /gene="gatB"
                     /locus_tag="RHZ76_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HDV2814457.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCWVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            2941..4296
                     /locus_tag="RHZ76_003909"
     CDS             2941..4296
                     /locus_tag="RHZ76_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol permease IIC component"
                     /protein_id="HDV2814458.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            4344..5384
                     /gene="gatD"
                     /locus_tag="RHZ76_003910"
     CDS             4344..5384
                     /gene="gatD"
                     /locus_tag="RHZ76_003910"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDV2814459.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRNNVFALPADMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSREMSAP
                     QMQGVLRELRFNQLILETTGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            5490..6263
                     /locus_tag="RHZ76_003911"
     CDS             5490..6263
                     /locus_tag="RHZ76_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV2814460.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(6345..7244)
                     /gene="yegS"
                     /locus_tag="RHZ76_003912"
     CDS             complement(6345..7244)
                     /gene="yegS"
                     /locus_tag="RHZ76_003912"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HDV2814461.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT         1891 a         1684 c         1960 g         1971 t
ORIGIN      
        1 gacaggatat gaaaatgtac gtggtatcga caaagcagat gctgaacaac gcacagcgcg
       61 gcggttatgc ggttccggca ttcaatattc acaatctcga aacgatgcaa gtggtggtag
      121 aaaccgctgc caacctgcat gcgccggtca tcatcgccgg aacgcctggc acatttactc
      181 atgctggtac agaaaatctg ttggcgctgg tcaacgcgat ggcgaagcaa tatcaccatc
      241 cactggcaat tcatctcgac catcacacga aatttgacga tatcgctcag aaggttcgtt
      301 ctggcgtgcg ctcagtcatg attgacgcct cgcatttgtc ttttgcgcaa aatatttcac
      361 gggtcaaaaa ggtggtggat ttttgccatc gctttgatgt cagtgtcgaa gcggagctgg
      421 ggcaacttgg cggccaggaa gatgatgtgc aagtcaatga agccgatgct ttttatacca
      481 accccgctca ggcgcgtgaa tttgccgagg caaccggaat tgattccctg gcggtcgcca
      541 tcggcacggc tcatggtatg tatgccagcg caccggcgct tgatttttct cgactggaga
      601 acattcgcca gtgggtgaac ttaccgctgg tactgcatgg cgcgtcaggg ttatcgacta
      661 aggatattca gcaaaccatc aaactgggga tatgcaaaat caacgttgca acggagctga
      721 aaaatgcctt ctcgcaggcg ttaaaaaatt acctgaccga gcaccccgaa gcgaccgatc
      781 cccgggatta tttgcagtcg gctaaatccg caatgcgcga tgtggtgagc aaagtgattg
      841 ccgattgtgg ctgcgagggc agggcataac gcacctgcca tttaacaagg aaaaaacatg
      901 aaaacgttaa ttgcccggca taaagctggt gaacatatag gcatatgttc agtctgttct
      961 gcccatccgt tggttatcga agcggcgctg gcatttgatc gcaacagcac gcgcaaagtg
     1021 ctgattgaag caacgtcaaa ccaggtcaat caatttggcg gttataccgg aatgacaccg
     1081 gcggactttc gcgaatttgt ttttacgatt gccgataaag ttgggtttgc acgcgaacgc
     1141 attattctcg gcggcgatca tctggggcca aactgctggc agcaagaaaa tgcggatgcg
     1201 gcgatggaaa aatccgtcga gctggtaaag gcatatgttc gtgctggctt cagtaaaatt
     1261 catcttgatg cgtcaatgtc ctgcgcgggg gatcccatac cgttagcacc agaaacggtt
     1321 gcggaacgag ctgctgtgct ttgctttgct gcggaaagtg tggcgacaga ttgccagcgt
     1381 gagcaactga gctatgtcat tggcaccgaa gttccggttc cgggcggtga ggccagcgcc
     1441 attcagtcag tacacatctc ccaggttgaa gatgccgcca atactttacg cacacatcaa
     1501 aaggccttta ttgcccgtgg gctgacagag gcgttaacac gcgtgattgc catcgtggtg
     1561 cagccgggtg tggaatttga tcacagcaat attatccatt atcagccgca ggaagcgcag
     1621 gcgctggcgc aatggataga aaacacccga atggtttatg aagcacattc taccgattac
     1681 cagacccgga cggcttatcg ggaattagtc cgcgatcact ttgcaatatt gaaagtcggt
     1741 cccgcattaa cctttgcttt acgtgaggcg atatttgcgc tggcacaaat tgagcaggaa
     1801 cttatcgccc ccgaaaatcg cagcggttgc ctggcggtaa ttgaagaagt gatgctcgac
     1861 gaaccgcagt actggaagaa atattatcgc acgggtttta acgattcatt actggatatt
     1921 cgttacagcc tgtcggatcg tattcgttat tactggccgc atagtcggat taaaaatagc
     1981 gttgaaacga tgatggtgaa tcttgaaggc gtggacatcc cactgggcat gattagtcag
     2041 tatcttccca aacaatttga acgcattcag tccggggaat tatcagcaat accgcatcag
     2101 ctgattatgg ataaaattta tgatgttttg cgcgcctatc gctacggctg tgcggaataa
     2161 ggacggtata tgactaacct gtttgttcgt agcggaattt cttttgtcga acgtagcgaa
     2221 gttttaaccc atatcggtaa tgagatgctc gcgaaaggtg tggttcatga tacctggcca
     2281 caggcattaa ttgccagaga agcagaattc cctaccggga taatgcttga gcagcacgcc
     2341 attgcaatac cgcattgtga ggcgattcat gctaagtcgt cagccattta tctgttaagg
     2401 ccaacaaata aagttcattt tcagcaagcg gatgatgata acgacgtgga ggtatcgttg
     2461 gttattgcgt tgattgtgga aaatccgcag cagcaattga aacttttgcg ctgtttattt
     2521 ggcaagttac aacagcccga tatcgtcgag acactaatta ctcttcctga aactcagtta
     2581 aaggaatact tcacaaagta tgttttagat tcagacgaat aaatccctct gtaacaataa
     2641 aaaggactat ttatgaaacg caagattatt gtcgcttgcg gaggcgcggt tgcgacctcc
     2701 acgatggcgg cggaagaaat taaagagttg tgtcagagtc ataatattcc tgttgaatta
     2761 atccagtgtt gggttaatga aatagaaacc tatatggatg gcgtgcattt gatatgcacc
     2821 actgccaggg tggatcgtag ttttggcgat attccgttag ttcacggcat gccttttgtt
     2881 tctggtgtcg gtatcgaagc attacaaaat aaaattctga ctatcttaca ggggtgacct
     2941 atgttttcag aagtcatgcg ttatattctc gacctcggcc ctacggtgat gctgccgatt
     3001 gtcatcatta ttttttctaa aatattaggc atgaaggcag gcgattgctt taaagcgggt
     3061 ctgcatatcg ggattggctt tgttggcatt ggccttgtga ttggcttaat gctggattcc
     3121 attggtccgg cggcgaaagc gatggcggaa aatttcgacc tgaatctgca tgtggtcgat
     3181 gtcggctggc cgggctcttc accaatgacc tgggcgtcgc aaattgcgct ggtggcgatt
     3241 ccgattgcga ttctggttaa cgtcgcgatg ttactgaccc gtatgacgcg ggtggtaaat
     3301 gttgatatct ggaatatctg gcatatgacc ttcaccggcg cgttgctgca tctggcaacc
     3361 ggttcatgga tgatagggat ggcgggtgtg gtaattcacg cggcgtttgt ttataagctc
     3421 ggcgactggt ttgcccgtga tacccgaaat ttctttgagc tggaaggcat tgctattccg
     3481 cacggtacgt cggcgtatat gggaccgatt gcggtgctgg tcgatgctat catcgagaaa
     3541 atcccaggcg ttaaccgaat taaatttagc gccgacgata ttcagcgtaa atttggtccg
     3601 tttggcgagc ctgtcaccgt gggttttgtg atggggctga ttatcggcat cctcgcgggt
     3661 tacgatgtca aaggtgtatt gcaactggcg gtaaaaacgg cggcagtcat gctgctgatg
     3721 ccacgggtga ttaaacccat catggatggc ttaacaccta ttgcgaagca ggcgcgtagc
     3781 cgtttgcagg cgaagttcgg cggtcaggag ttcctgattg gcctagatcc ggcattactg
     3841 ctggggcata cggcggtggt atcggcaagc ctgattttta tcccgctcac cattttaatt
     3901 gctgtttgtg ttccgggtaa tcaggtactg ccgtttggcg atcttgccac catcggcttc
     3961 ttcgtggcga tggcggtcgc ggtgcatcgt ggaaatctgt tccgcacctt aatctcgggt
     4021 gtcatcatta tgagcatcac cctgtggatc gcgacgcaaa ctattggttt gcacacccaa
     4081 ctggcggcta atgctggggc gttaaaagcc gggggtatgg tggcttcaat ggatcagggc
     4141 ggttctccca ttacctggtt actgattcag gttttctccc cgcaaaatat tcccggtttc
     4201 attattatcg gcgcaattta tctgaccggt attttcatga cctggcgtag agcgcgtggc
     4261 tttattaaac aagagaaagt cgttctcgca gaataatttt tacctgaggg ggaggttatc
     4321 ccccttaata atcaggagtt tttatgaaat cagtggtgaa tgatactgat ggtatcgtgc
     4381 gcgttgcaga aagcgtcatt cctgaaatta aacatcagga tgaggtgcgg gtaaaaattg
     4441 ccagctcggg cttatgtggt tccgatttac ccaggatatt taaaaatggt gcacattatt
     4501 atccaataac gttaggccat gaatttagcg gctatattga tgcggtggga tccggtgttg
     4561 atgatttaca tcctggcgat gcggttgcct gtgtgccgtt attaccctgt tttacttgtc
     4621 cagagtgtct gaaagggttt tattcccagt gcgcaaaata tgattttatt ggctcacggc
     4681 gtgatggtgg atttgccgaa tatattgtcg ttaagcgaaa caatgtcttt gcactacctg
     4741 cggatatgcc tattgaggat ggggctttta ttgaaccgat taccgttggc ctgcatgctt
     4801 ttcatttagc gcaaggttgc gaaaataaaa acgttattat tattggtgcc ggaaccattg
     4861 gcctgctggc cattcagtgc gctgtcgcgc tgggagcaaa gagtgtgacg gctatcgaca
     4921 ttagctcaga aaaactggca ctggcaaaat ctttcggtgc gatgcaaaca tttaacagcc
     4981 gtgaaatgag cgcgccgcaa atgcaggggg ttttacgcga gctgcgcttt aatcagctta
     5041 tcctcgagac gactggcgta ccgcaaactg tcgaactggc ggtagagatt gccggtcctc
     5101 atgcccaact ggcgctggtg ggcacgttgc atcaggatct gcatttaaca tcggcaacgt
     5161 ttggcaaaat attgcgtaaa gagttgacgg ttatcggcag ttggatgaac tactccagcc
     5221 cttggccagg gcaggagtgg gaaacggcga gccggttgct gacagaacgt aagttaagcc
     5281 tggagccatt aatcgctcac cgtggaagct ttgaaagctt cgcccaggcg gtgcgtgaca
     5341 tcgctcgtaa tgctatgccg ggcaaagtgt tgctcattcc ctgaaaccgc gggccagcat
     5401 gatgctggcc cggtattgtg caaaacagat cattcaccaa tggtccccct tcgtttacac
     5461 tagccacaat tgaatatggg taaatgacga tgaattcatt cgagcgaagg aataagatca
     5521 tccaattagt gaatgaacag ggaaccgtgc ttgttcagga tctggcggga gtatttgctg
     5581 cctcggaagc gacaatccgt gccgatttgc gctttctcga acaaaaaggc gtggttacgc
     5641 gctttcatgg cggtgcggcg aaaataatgt ccggtaatag tgaaaccgag acccaggaag
     5701 tcgggtttaa agagcgtttt cagctcgcca gcgcgccaaa aaacagaata gcgcaggcgg
     5761 cagtcaaaat gatccacgaa gggatgaccg ttattctcga cagcggaagt acgacaatgc
     5821 ttatcgccga aggattaatg accgccaaaa atatcacggt gattaccaac agtctcccgg
     5881 cagcgtttgc cctttccgaa aataaagaca ttactctggt cgtctgcggt ggcacggtcc
     5941 gccataaaac gcgctcgatg catggttcca ttgccgagcg ttcattgcag gatattaatg
     6001 ccgatttaat gtttgtcggt gctgatggta ttgatgcggt taatggtatt acgaccttta
     6061 atgaaggtta ttcgattagc ggggcgatgg tcaccgccgc taataaagtg attgccgttc
     6121 tcgattcatc gaaatttaac cgtcgcggtt ttaatcaggt actgccaatc gaaaaaatcg
     6181 acattattat taccgacgat gctgtgtcag aggtggataa actggcattg cagaagacac
     6241 gggtaaaatt aattacggta tagtattgtt ctgtatgccg gataaggtgt ttacaccgca
     6301 tccggcatta cggattctcc ctggttcaag agagaatagt ttgattaacg caataatgga
     6361 caatctggtg gtaatcgaca acgcaacgcc gccggaagta tttcaatatg gaagttttgc
     6421 ccactcaacg gttcgccatc aagattaaaa gtgatttcgt gtggggcttg tatatcaaac
     6481 cacgacgaag cgccttcgat aatattcggg ttatcttcgt cagattttaa ggttgatacg
     6541 agagctggaa gaatttcatc gccggtaaaa atgcgcagtt gcagcaagcc atcgttaatt
     6601 aacgcgttcg gacacaattg ctgaccgcca ccggcctgac gcccgttacc aataccaatg
     6661 accagggcgt caccttgcca gtgaaagttt tcaccgcgga tttcacaacg gtctggttgc
     6721 agagtatcca tgcgcattaa gccatgaatg atgtaagaga cgccacccag cgcggctttt
     6781 aatttttccg gcgtttctgt ggtaatacgc gtcccaaatc cgcctgtcgc catattaata
     6841 aaacaggttt gtttgttgac ctgcgccata tctatcgcaa tggcgttacc ggcaattgcc
     6901 agtttcagcg ccttatccag tgcctcagga atccctacac tggtggcaaa atcattggca
     6961 gttcctaatg gcaaaattcc cagcgcgggt atgtcatccc cctcacactg aatcaacgcc
     7021 gtagaaactt cattaatggt gccatctcca ccaccggcaa tcaccgttgc gacgcccaac
     7081 ttccgggcct cctctacata tcgtgcggca tcgcctttct cccaggtgac ccgcacatgg
     7141 atcgtcattc cttcctcacg caacagcata attgcttcgc gcaagggtag attgtcagta
     7201 cttttgccat taagaatcag taagctggcg ggaaattctg ccatgatcgt atgtgccttt
     7261 atgattggtc tgtagatagt gtagagcaga aaagaaaagg tggagtcaga acaggatgaa
     7321 agtcggagga taaaaagtca gttcaagtat ggaggattca gtggttacca ccaggagctt
     7381 gcaatgggaa cagcgaatat taacggtagc caccgataaa tttcaaaaaa gagcatatgc
     7441 ctaatattca accaaacagt ggcatcttca atatactatt ccaaatatat taaagcccca
     7501 tggagt
//
LOCUS       DAPHBJ010000208         7299 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.210, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000208 DAPHBJ010000000
VERSION     DAPHBJ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.210"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..987)
                     /gene="pyrD"
                     /locus_tag="RHZ76_003913"
     CDS             complement(<1..987)
                     /gene="pyrD"
                     /locus_tag="RHZ76_003913"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDV2814462.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLI"
     gene            complement(1098..1836)
                     /locus_tag="RHZ76_003914"
                     /pseudo
     CDS             complement(1098..1836)
                     /locus_tag="RHZ76_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(1802..2317)
                     /locus_tag="RHZ76_003915"
     CDS             complement(1802..2317)
                     /locus_tag="RHZ76_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV2814463.1"
                     /translation="MLKRIIWILFLLGLTWGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(2325..2867)
                     /locus_tag="RHZ76_003916"
     CDS             complement(2325..2867)
                     /locus_tag="RHZ76_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2814464.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(2879..3949)
                     /locus_tag="RHZ76_003917"
     CDS             complement(2879..3949)
                     /locus_tag="RHZ76_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2814465.1"
                     /translation="MQIIFGEKCVSLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEANTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGGDTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLYECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            complement(3940..6540)
                     /locus_tag="RHZ76_003918"
     CDS             complement(3940..6540)
                     /locus_tag="RHZ76_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HDV2814466.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVELTPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACALLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGGSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGVQANSGIIDDDGSLYMAGLPAKGTISVRWGEAPDQICHINYELTEQQINS
                     AITRMDAICR"
     gene            complement(6565..7266)
                     /locus_tag="RHZ76_003919"
     CDS             complement(6565..7266)
                     /locus_tag="RHZ76_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV2814467.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLGYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLAEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIDGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
BASE COUNT         1855 a         1879 c         1618 g         1947 t
ORIGIN      
        1 aatcagcggc ggacctttaa aaataaaacc agaataaatt tgcaccagtg aggcacccgc
       61 agcaatcttt tcacgcgcag cgataaccga gtcgatgccg ccaacaccga tgatcggtaa
      121 gcgaccgttt aattccagtg acaagcggcg aataatttcg gtgcttttta actgaagcgg
      181 acgaccactt aagccaccgg tttgatcgca atttttcatt ccctgaacaa gagaacgatc
      241 gagtgtggta ttggttgcaa taacgccatc aatattatgg cgaactaaac tatcggcaac
      301 ctggatcaat tcttcttcag aaagatccgg cgcgatcttc actgcgatcg gcacatattt
      361 atggtgcatc gcttgcaaat cattttgctt atttttaatc gcggttaaga gatcatccag
      421 cgcttcacca tattgcagcg tgcgtaatcc tggggtattc ggcgatgaaa tattgatggc
      481 gatatatccc gcataggcat agattttttc catacaaatc agatagtcat ctttgccctg
      541 ctccactggc gtatctttat ttttgccgat gttaataccc aggacgccgt cataatgggc
      601 cttttttacg ttctctacga ggttatcaac gccaagatta ttaaagccca tacggttgat
      661 caaaccttcg gcatctacca gacgaaagag acgcggcttg tcattacctg gctgtggacg
      721 tggcgtgacg gtaccgatct cgatcgatcc aaatcccatc gcgcctaacg cgtcaatgca
      781 ctccccgtct ttatcaagac cggctgccag accaagcgga tttttaaacg tcaggcccat
      841 gcagttaaca ggtttcgcag gcactttctg ccgcaccagt gcttcaaacg gcgttcctgt
      901 aatacggcgt aattgctgaa aagtaaactc atgagcgcgc tctggatcga gctggaaaag
      961 ggctttacga acgaaggggt agtacatgaa ctctcctgga ttcccggtgt gcaaaccggg
     1021 ggcgtattat gtgcgagttc ggaccaaaag ggaattgacc tgcggcaaaa aaaacgcaaa
     1081 cgttttccga acctgtttca gggcagattt tgctccttct ttgcactcaa cccgccgtaa
     1141 tcatttatcg cccgccaggc gacatgacac ggtgcgattg cctgacacca gcttgtctga
     1201 cgctggcttt taggcggcac aactcccaca ttacttaacg ctttaccatt tacactcacc
     1261 tgaatcaggt taatgtaata aggcgttggg ttagtgagtt gtacaccctg cgaattccgt
     1321 gtccagcgta attgttgata agcttccagc gggtcgcccg gcaaaccttc gggtcgccag
     1381 aacagtttaa ataccgagcg catcgccact tttacgctct gattttcaac tttgccggat
     1441 ggcacgctgg caatgcttaa ctcaaatagc gtttcgcgat ccacaggcaa gatgtcgctt
     1501 tccgttctca gcaagcgcaa cgtgcctgtc gtaccgggct tcaggagaat aagtggcgga
     1561 gcggccagta atggtgctgg cactgtcgac gcttcccccc ccggcccaac gcgttgggcg
     1621 gttgatttta ctgttaataa gccaggattc ctgactggta ttagtcagta aaatagatat
     1681 cgattctctg tctgccggaa agataaatcg cgttccgccg accaccactc ctgcctgcaa
     1741 ttcacccgcc accagcagcg ataaaacggc gaaaatcaag agcgccaatc tgttccacgt
     1801 attagtcata atgaagtaca aatgtcgcgc tggcattcac tttacccgac tgcacatcgc
     1861 tattgacagg tcgcaattgc gcataaaaag ttaatggcac cttaccgcta tgtaggctgt
     1921 agtctttact cgcctggtta agcggaatca atatcttcgc atcatccaga atcgcaattg
     1981 ccagcccgct ggctgcatcc gtaccacttt caatggacaa catttcgctc tgcactccgt
     2041 cgggggttcc tgaaaaggtg acgtcaaccg tgctgacatc tgttccgcaa tcctgcaaat
     2101 taatgatgaa agatttctcc gggccataat tcccatgaga aaaacgtgtc agcgagacat
     2161 cgcctaactg cacattgatt tgcttgctat cctgtgccag cacgcatgta ttacggcgaa
     2221 acgatccgct aatgttgacc gtgccatcat gggcaaatag ctcacagccc cacgttaatc
     2281 ccaataagaa cagaatccag attatccgtt tcaacatcac tccttcactg gtaaaccaga
     2341 tcaaaataga gaaccgccgt cgcattaccg cccgttgctc ccgcctttgt ggacttataa
     2401 cgaagttgat atttgagtgt gttatccccg gcttttaagg gcgtaagagg ctggacctga
     2461 ttgagcggga tttcttgctg actttgcgca tcaagaattt gcacggcaat ccccgttgcc
     2521 ataccaccgc ttccgcctgt gtcagttagt ttgagcagtg tcggcgcaag ggtgtcagcc
     2581 tcgccgctaa aaaggacgtc cgcgccagta ataccagcgg cacaaccggt gatatcgata
     2641 ttcaaatcag cagcagtgct tacggtattc gcggcctgaa aatcactgat attaaaatca
     2701 ccaatatgaa tattttgtaa tgcacttttg gtggcgacat cacagccctg atcgaccaca
     2761 ttcccataaa tatgcatact aacttgttca ggcccagcca tcccgacatg gatgttaaga
     2821 atgctaaaga acaaaataac gcactgaaat atcgtttttt tcttcatacg ttcacgcgtt
     2881 aatcaaaatt tattctcacc gttgccgtgg cttcaaatcg cccggtttct ggtggtacac
     2941 ctgtcgtgct gactggatag acattcattc gcgcgatact gtctgcatca tcaggaatag
     3001 agagcggtaa tgttccacca gcaggggaaa taatgttgtt ctggctatcg gtcaccacca
     3061 cgcccacgcc aggacgtgac gtcttaatcg cctggggata gctggggtct gtggttgccg
     3121 tcagactcag gttgacatta actaatcccg cgtttcccgt gttacacctg actggaattg
     3181 caagttctgc tgttctggcc ccaagaggtt tatgccctac aacacggaaa ttggcagcga
     3241 agacatcacc taaatcaact tcaattgtct ctccggcatt gacggaacat gagccaagtg
     3301 aattgatcgt accagacagg taatacacta aaaccggtgt acctgtagtc gtgcaggaat
     3361 cacctgcggg tatgttgtag cattcatata atcgcgccag cgccatagga ggaatgacga
     3421 ccgagccaac gaacggctgc aatattttca gggaaaagct accgctatta ccgcttgccc
     3481 cctgcccacc taaacgttga ccgccagggt cgcaaatatg ccatcgggta tccccccctg
     3541 tgcccacgtt ttcgaaagga accgcagtag gagaactgcc tttaacctgc aataaaactt
     3601 tggcggcaag atagtcatta attttgtacc agttgtcgcc taaatagact aacggtgaat
     3661 ccgcggcgaa tgcccatcgg ccacttctga cattgtcttt atcgcaatca caggaaacat
     3721 aataatcatt cgattcgttc cactgataaa atttgtcttt aactacccct gccgtatttg
     3781 cttcttcctc cagggtttta acgccaaaat tgacgccaat atacggattc ccctgagtag
     3841 tatgacactg cccgctataa gcagcggttt gagcgcacca tagcattaag acggcggcaa
     3901 aaaatagtcg tagtaatgac acgcattttt ctccaaagat tatctgcata tggcatccat
     3961 tcgcgtaatc gcagagttaa tttgttgttc ggtaagctcg taattgatat gacaaatttg
     4021 atcgggagct tcgccccagc gcacgcttat tgttcccttc gccggtaaac ccgccatata
     4081 gagcgagcca tcatcatcaa ttatgccgct attagcctgt acgccgtcca gcgtcgctat
     4141 cgcgccaaaa cgcagcggta taccctgctt tgatgttttc attaataccc ttgcacccgc
     4201 atgggcattg aactccgcca gaaccaacgc ccctttggtg ggaaccacgt tgactactgc
     4261 attttcaaga tccacgttac gttttaacga cgccgcatca agtgcgattc gattttcccg
     4321 ataagaagtg gcataaggaa caacggtata tccgcgccag tcagttttca gcccggtgct
     4381 gttcgccaca cgagtattct ctgcgcctgg tgctgcaatc agaacgttag tttcccccag
     4441 cgattgcccc agggtaatgc cctgtgaatg ggcaactaaa ctgcctgaaa gagcgtagtt
     4501 cagttgttgt tgactgccgt tatcgctgta gttgtagccc actcgggcat ctgcaaacgc
     4561 ccctttgtag tccatgctgg cgctaccatt agccgttttc ccctcgctgt tatatccctg
     4621 ttgcacgctg tagctcaggt taccgtcatc gagtagtgtc tccgttaaac ccaggttttg
     4681 tgtaatgtgc ccttcgttat cgatactggt gttagaggtc atccaggcgt aatttttttt
     4741 cctggtatag ctatcattac ttctggggag caacagattg agcggcaggg aaaaatttag
     4801 tgcaaacacc tgatcagcat caggttgacc acgggactta ctgtagttcc aggaaccccc
     4861 cagcgagaga tctttaattt gcgtgttgta gccaaactgt aatagccgat cctgttgatc
     4921 ggtatgccag taagtttgct ggctaccact taaataaaac gaaccgtact cgcctaattg
     4981 ctgggagata ttgacctgca gtttgccacg tttggtgtaa aacaaattgt aataacgcga
     5041 ccacatcggc gtatcttcat catcaccgtc attaaattcg tagccatcca tatgtttata
     5101 catggtgtcg gaaagggtgt agaaacccga cgtcgaatag cgatagccca tcaattgaaa
     5161 attagtgccc aattggttga gcgttttggc atacagaaaa cgatatgact gacctttatg
     5221 ttcgctttgg tctgccagcg tactcttcgc ctgggtcgca tcgaacgaaa ttgctccgaa
     5281 atcgccaagg ttaaatccca gaccaaacat ggcggcacgg taatattcag catattgtcc
     5341 accaccatac catgtcgttc cccacggtcc gccccactgc aacgtggcct gggcaaattt
     5401 gctctcctgc tgttcattac tattggttct gtatttcgcc agtgtcaccg cgtatttgat
     5461 tcgcccctga cgctggagta atggcacgct ggaaaacggt acgctgtagc tattgacgct
     5521 gccgtccgct tctttgattt caactaacaa atcaccgctc gacgacgtgg aatagagatc
     5581 actaatttca aaagcaccag gcgatacata agtttggtaa atggtgtaac cattttgctt
     5641 aatcgttatc tgggcattgc ttttggcgat accacgcacc acaggcgcaa accctttcag
     5701 gctgtctggc agcatgttgt catctgacgc cagctgcaca ccacgaaagc tgacactatc
     5761 gaaaaaatcg cctgcagtat aatcatcacc tacggtcagt tcgcctttta gtggaataac
     5821 cgcccgctgc aaatagctgc tgagattctt ccattctgcg gtttggccac tactgcgact
     5881 ccatgttgaa ttgttgcgca atctccatgg gcctaaatta acgccactgt ttaaattcag
     5941 aaaataactg tcgccagaat cactgtctgc gctgctatga atactgttag ccccgctaaa
     6001 tgaatatccc agtaatagcg cgttgatccc ttcatcccaa cgttcagggg ggacataacc
     6061 acgagcgtta ctttttatag caatttgcgg tactgaaatt tccagacgga gtttattcac
     6121 attaaaagtt acgctggcat ccggaataat ctccgccaat agcgcacacg cttgtttgtc
     6181 cagttgttta aaagccggaa acgcatcagt attcacaccc attgcatcca ggctttcagt
     6241 cgtcaaacag gcggcaatcc ccttctctgg cgttaactca acaaaattga cgttgcgcgt
     6301 atctacaatt gtctgattca gaacaatatc gacacgataa acccctggcg ttattttttg
     6361 tcctttttcg aaacgagaca aatcggccac cattgaggga tcatctttga aaaaggcagg
     6421 atcgaaccag gtttcagctt gtgcattgaa cgctgccgac acaaacacca ccagcccacc
     6481 aataaacgat ggcactccac cagagcttaa caggctgtgt tgtcggtgag ttctatacat
     6541 ctcgctctct taactgaaga cgaattacga tttacagttg agttgcggcg tttccgcccc
     6601 gtagtcattg atggttcgat acgttacgct gctatttttg gcaccctttg gcagagagaa
     6661 ttggtattta tcaaaggggg aaatcatttg atttttaact acctttccat ctattttcag
     6721 gtctgtcagg gtaatgaaat aaggtgttgg gttactgacc gtaaccatgt cagctgacga
     6781 agtgcaacgc agtttcgccg gagcctcggc taagggatac gctaaacctt ttggtcgcca
     6841 gaaaagtttg atgacgcttt gtgtggcaat cttcagggta tttccctctt cccgcttcgt
     6901 tgttgaaggg actgcccgta cgctagtgaa gaaaagggtt tctctgtctt tcgccaacgg
     6961 cgctccaatg tacataatgc gtaacagatt ctcatttgcc ggattcagca caaatagcgg
     7021 tggtgtaata ataaagtctg ctgatttttt attatctggg gtcatcaccc atgactgaat
     7081 aagataagga acatccggac tggtgttacg tacacccagt gaaatttgct cttcaccttc
     7141 tgaataacca agacgtgttg ctgcaagtcc aatcccgcct ttacctgccg cccatgcgga
     7201 tgagcagctg agtaatattg ccgttaaact tacggtcaca attccctttg ttatgcaagt
     7261 tttcataaat agtcactctt aaaaaatatg aatatcccc
//
LOCUS       DAPHBJ010000209         7281 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.211, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000209 DAPHBJ010000000
VERSION     DAPHBJ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.211"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..343
                     /locus_tag="RHZ76_003920"
     CDS             <1..343
                     /locus_tag="RHZ76_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDV2814468.1"
                     /translation="AWHTRLMEDGVQAMRDYLAGLDIASPEHQVLMNVTAKSEVAPSI
                     IKENLSLHLTHTVKWTESLDTFLNMPTPVAFLEISNKPYLGNMLNDFAGVDQQRVMHC
                     RKAFSDAKVFK"
     gene            340..1326
                     /locus_tag="RHZ76_003921"
     CDS             340..1326
                     /locus_tag="RHZ76_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814469.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFIFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEVMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFYCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(1519..1606)
                     /locus_tag="RHZ76_003922"
     tRNA            complement(1519..1606)
                     /locus_tag="RHZ76_003922"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1570..1572),aa:Ser,seq:gga)
     gene            1840..2778
                     /gene="ghrA"
                     /locus_tag="RHZ76_003923"
     CDS             1840..2778
                     /gene="ghrA"
                     /locus_tag="RHZ76_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HDV2814470.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGEKVCGQVDRARGY"
     gene            2833..3570
                     /gene="ycdX"
                     /locus_tag="RHZ76_003924"
     CDS             2833..3570
                     /gene="ycdX"
                     /locus_tag="RHZ76_003924"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HDV2814471.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            3594..4148
                     /gene="ycdY"
                     /locus_tag="RHZ76_003925"
     CDS             3594..4148
                     /gene="ycdY"
                     /locus_tag="RHZ76_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HDV2814472.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            4250..4741
                     /locus_tag="RHZ76_003926"
     CDS             4250..4741
                     /locus_tag="RHZ76_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDV2814473.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4805..5638)
                     /gene="csgG"
                     /locus_tag="RHZ76_003927"
     CDS             complement(4805..5638)
                     /gene="csgG"
                     /locus_tag="RHZ76_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HDV2814474.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(5665..6081)
                     /gene="csgF"
                     /locus_tag="RHZ76_003928"
     CDS             complement(5665..6081)
                     /gene="csgF"
                     /locus_tag="RHZ76_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HDV2814475.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(6106..6495)
                     /gene="csgE"
                     /locus_tag="RHZ76_003929"
     CDS             complement(6106..6495)
                     /gene="csgE"
                     /locus_tag="RHZ76_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HDV2814476.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(6500..7150)
                     /gene="csgD"
                     /locus_tag="RHZ76_003930"
     CDS             complement(6500..7150)
                     /gene="csgD"
                     /locus_tag="RHZ76_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HDV2814477.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYSMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT         1816 a         1610 c         1783 g         2072 t
ORIGIN      
        1 tgcctggcac acgcgtttga tggaagacgg cgttcaggca atgcgcgatt atcttgccgg
       61 actggatatc gcttcgccag agcatcaggt tctgatgaac gtaacagcaa aaagcgaggt
      121 cgctccgtca attatcaaag aaaatctctc attacactta actcatacgg tgaaatggac
      181 tgaatctctc gatacatttc tgaatatgcc aaccccggta gctttccttg aaataagtaa
      241 taagccctat ttggggaata tgttaaatga ttttgctggc gtggatcagc aacgagttat
      301 gcattgtcgt aaagcattta gtgatgcaaa ggtattcaaa tgattcctga ttatttaact
      361 tttattcgct ttcaggataa acgaaatctg atatacattt atgctattgg acttattctg
      421 ataggctttt attggaagaa tgcagggttt atttttccat cagaggatat tggtgtagtt
      481 agtgggattc tggctctggt gctgtataat tttatttttg atctcaaggc gtactgggct
      541 tataaatgcg tcacgaagaa tatcgatttt tcgtggttta agaaaaagca gaaccacaaa
      601 atagaattat ttcttacaca acctctggtg gcaggatttc tgtcgttaat catgttgagt
      661 gcaatgagtt gggggctata ccagcttcta ccctcgttat atgcgctgtt cctgatttcg
      721 ttacttgggc cgttggtcat ctttctgctg tttcggatga tccgcaccag ttatgtcaag
      781 caggtcgcta tttcagtagc gaaaaaagta aaatataaaa gtctgactcg ctatgtgctg
      841 ctttcggtgt gcatctcaac ggttgttaac ctgcttacta tcagcccgtt gcgtaacagt
      901 gattcttttg tgacagaggg gcagtggtta acgtttaaat cgataattgc attgctcatt
      961 ctttgtggcg tagtgttggc gattaatctg ttttttctgc gcttctccaa gcggtacgct
     1021 tttctgggca ggcttttttt gcaggaaatc gatctgtttt tctccagtga aaatgcgttg
     1081 tcgacctttt ttgccaagcc gctttggctt cggttattca tattgctggt tattgaagtg
     1141 atgtggatta cgctggtgtc ggtattggca acgcttgtgg aatggcggat ttggtttgaa
     1201 gcctattttt tactctgcta tgtaccgtgc ttaatttact attttttcta ttgtcgattc
     1261 ctctggcata acgattttat gatggcatgt gacatgtatt tccgttgggg gcattttaat
     1321 aagtgaggaa gtgataggaa gtgaccagat aatacatata tgttctgtac tctcttgcgc
     1381 attttgattg ttgactgagt aaccagacag ttgatgtgca cgatttcccc tcgccctaac
     1441 agacgtgggc gggggcaccc ggtgtgtgca atatacgaaa aaaaagcccg tactttcgta
     1501 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     1561 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgtcgttcct
     1621 gtcttgctgg aacgggcgct aatttaggga aatcatgacc tgaggtcaac aaactttttt
     1681 aaaaaatcgc acgtttattc aaacttcaat caatatgcag ttttaataag cgaaatctgc
     1741 tttttttgcc accgaccacg gatttgttat gctggtggct tttgtagatc ataacgataa
     1801 gtgcgaataa atttcgcaca acgcttttcg ggagtcagta tggatatcat cttttatcac
     1861 ccaacgttcg atacccaatg gtggattgag gcactgcgca aagctattcc tcaggcaaga
     1921 gtcagagcat ggaaaagcgg agataatgac tctgctgatt atgctttagt ctggcatcct
     1981 cctgttgaaa tgctggcagg gcgcgatctt aaagcggtgt tcgcactcgg ggccggtgtt
     2041 gattctattt tgagcaagct acaggcacac cctgaaatgc tgaacccttc tgttccactt
     2101 tttcgcctgg aagataccgg tatgggcgag caaatgcagg aatatgctgt cagtcaggtg
     2161 ctgcattggt ttcgacgttt tgacgattat cgcatccagc aaaatagttc gcattggcaa
     2221 ccgctgcctg aatatcatcg ggaagatttt accatcggca ttttgggcgc aggcgtactg
     2281 ggcagtaaag ttgctcagag tctgcaaacc tggcgctttc cgctgcgttg ctggagtcga
     2341 acccgtaaat cgtggcctgg cgtgcaaagc tttgccggac gggaagaact gtctgcattt
     2401 ctgagccaat gtcgggtatt gattaatttg ttaccgaata cccctgaaac cgtcggcatt
     2461 attaatcaac aattactcga aaaattaccg gatggcgcgt atctcctcaa cctggcgcgt
     2521 ggtgttcatg ttgtggaaga tgacctgctc gcggcgctgg atagcgggaa agttaaaggc
     2581 gcaatgctgg atgtttttaa tcgtgaaccc ttaccgcctg aaagtccgct ctggcaacat
     2641 ccacgcgtga cgataacacc acatgtcgcc gcgattaccc gtcccgctga agctgtggag
     2701 tacatttctc gcactattgc ccagctcgaa aaaggggaga aggtctgcgg gcaagtcgac
     2761 cgcgcacgcg gctactaata aagcatcagg attcctgcta tccttggcgg gaattgaata
     2821 caggagagag ttatgtatcc cgtcgacctt catatgcata ccgttgccag cacacatgca
     2881 tatagcacat tgagtgatta cattgcccag gccaaacaaa agggcattaa actttttgcg
     2941 atcaccgatc atggcccgga tatggaagat gctccgcatc actggcactt cattaacatg
     3001 cgtatctggc cgcgagtggt tgatggggta gggatcctgc gcggcatcga agctaacatt
     3061 aaaaatgttg atggtgaaat tgattgcagc ggtaaaatgt ttgactcgct ggatctgatt
     3121 attgccggtt ttcatgagcc ggtttttgca ccacatgaca aagcgaccaa tacacaagcg
     3181 atgatcgcca ctatcgccag cggcaatgtg catataataa gccatcccgg aaatcccaaa
     3241 tatgaaatag atgtgaaagc cgttgctgaa gcagccgcga aacatcaggt ggcgctggaa
     3301 atcaataatt cttcattttt acactcacgt aagggcagtg aagacaactg tcgtgcggta
     3361 gccgcagcgg tacgtgatgc tggaggttgg gtggcattag gctcggattc tcacaccgcg
     3421 tttaccatgg gggaatttga agagtgtctt aaaatcctcg acgcggtaga ttttccgcca
     3481 gagcgcattt tgaatgtttc tccgcgccgc ttactgaact tccttgaatc tcgcggtatg
     3541 gcaccgattg cggaatttgc agacctttaa ttactagtaa tggaaatata ttaatgaacg
     3601 agttttctat cctctgtcgt gtgctgggtt cgctctatta ccgccaaccg caagatcctt
     3661 tactggtgcc gctgtttacc ctgattcgtg aggggaaact ggctgcgaac tggccactgg
     3721 agcaggatga gttactgacg cgtttacaga aaagctgtga tatggcgcaa gtctctgccg
     3781 attacaatgc gttgtttatc ggcgatgaat gtgccgtgcc gccatatcgt agcgcatggg
     3841 ttgaggacgc aacggaagcg gaagtgcgcg cttttctttc cgaacgaggg atgccactgg
     3901 cggatacgcc agccgatcac atcggcacat tgctgcttgc ggcttcctgg ctggaagatc
     3961 agtcaacgga agatgagagc gaagcactgg aaacactgtt cagtgagtat ctgttaccct
     4021 ggtgtggtgc gttccttggc aaagtggagg cccatgcaac cacgcctttc tggcgcacca
     4081 tggcaccgct aacccgcgat gccattagtg caatgtggga cgagctggaa gaagattctg
     4141 aagaataagt gtgatctacg tcactcataa ctgcaacgga taatttgttg ttgcataaaa
     4201 tgtgtgctcg atctcattca tggccgcgtt ttctgctatc atgcgcggca tgaacatact
     4261 tctctctatt gcaatcacaa caggcattct ctccggtatc tggggatggg tggctgtttc
     4321 cctgggctta ctaagctggg cgggcttcct gggctgtacg gcctactttg cctgcccgca
     4381 aggtgggctg aaagggctgg cgatctccgc tgcaaccctg cttagtggcg tggtgtgggc
     4441 gatggtcatt atttacggta gtgcactggc accacatctg gaaattctcg gttatgtcat
     4501 aaccggtatt gtcgcgtttc tgatgtgtat tcaggccaaa cagctgctgc tttcatttgt
     4561 tccggggacg tttataggcg catgcgcgac gtttgccggg cagggtgact ggaaactggt
     4621 gttaccttcg ctggcgcttg ggctgatatt tggttacgca atgaaaaaca gtggcctgtg
     4681 gctggcggcg cgtagtgcaa agacggcgca ccgtgagcag gaaatcaaaa ataaagcgtg
     4741 aggggcactc acgctttcgc ttaaacagta aaatgccgga tgataattcc ggctttttta
     4801 tctgtcagga ttccggtgga accgacatat ggcggtattt caccagaatg tcattctgcc
     4861 gttctgcttt attttgcaaa tcccacagac cacggtcgat accatcatta atcaggaaaa
     4921 tgacccctgt ttcgatagcc gacatcaggc acagcataac aggttcgttc gaggtgtaac
     4981 ccacttcccc ttcaagcaag cgctggtagt caataaagcg gaaaaccccg gcctgaactt
     5041 cataggaaag tatcgtctta ctggtgttca ccgaagaaag gatctcgccg gtactcacat
     5101 tgacgacgcg caggttcacg gcaatctgat cgagctggta ttgcgtgtcg gcaccgatgc
     5161 caaaatatct tgccccaacc ccgccagatt tgacgttgct ttcataaccg ataatcgaac
     5221 cttcaaccat gatatttgcc gccgttaaag attgcagcgg gattcggtta ttaatggcaa
     5281 ccgtgccgtt ttcttgtgcc gcacgaataa tcttgcgctc gttaagcagg ttttgtaagc
     5341 cctggcgctc cagcggtata aaccagcgag aatctttcag tgccgtgacc agcattgccg
     5401 tggcgctttg cggaacagca gtggagaagt tacttgccgg gtagggttta aattgcccgg
     5461 tttcgtcctg aatgttgtat accgaaacaa agattttacc cgtcggcgct ggcagatggg
     5521 tcaaatcttt gtagctctga gcacgaggca ttaatgtcgg tctggcggct tctttaggcg
     5581 gggcggttaa gcatccgctc agtaacatga cggcaaccaa aagaaataag cgctgcatga
     5641 ttattttcct tatgaagctg gggcttaaaa atcggttgag ttattttgta aacccgaaac
     5701 ctggatggtc gaggtttgtc cggttttacg atctgtcacg ttcaactgca attgaccatc
     5761 gcggttggca atatcgacaa tataatcgtt ggtcaccatg cggcccggtt taccggtatt
     5821 aatattcgac agtagcccac ctaaaatttg tgactggatg gcctgagtaa agttatctaa
     5881 cgctgagggt gtttcaatac caaagtcatc gttatagctc ggatctttat aagagttttg
     5941 ggcctgagcg ctatttaata aaaaagcgcc attatttggg ttaccaccaa agtttggatt
     6001 acggaactgg aaagtcatgg ttccagccca acttaatggc gaaataagca tgagtagaac
     6061 tactgcatgt ttgacacgca ttgcagcctc cgaacaattt tttatttaga attcatcatg
     6121 cgccaaatcg cccgtactta ataacgcctg atttatctgg cgacgattta gtgcttcttc
     6181 agtttgaatc agtgcaaaga cgacagtttt ctcgaagtct cttttcaacg gaaataaaaa
     6241 agtctggaaa ataacgtcct gattgaccgt tatagtgatc cagcttcccc atcgtgcact
     6301 gggcctttca ttaatcgtta agttacccgt atagtcactt tcccatttat cactaaaggc
     6361 tcggtaaaaa tcatggccaa tagatgaaac agtatggtca gttagcaatc ccgggacttc
     6421 tacctcaacg gcgtgaagat tccctgcggc gaacagaaat tctgccgcca caatccagcg
     6481 taaataacgt ttcatggctt tatcgcctga ggttatcgtt tgcccaggaa accgcttgtg
     6541 tccggttttt tacggctatc ttcttgaaaa gattataaag atgcgtttta accgtatttt
     6601 cgctgatgaa caacgaacga gcgatctcgt tattagacgc gccgatacgc agcttattca
     6661 ggatctcttt ttcccgatga gtaaggaggg ctgattccgt gctgttataa cgatagttac
     6721 ctgaatgcgt aatcaggtag ctggcaagct tttgcgtaaa gtagcattcg ccgcgcagga
     6781 cgccttgcaa cccattgaca acacgttctt gatcctccat ggaataaaaa acgccgttga
     6841 tatgaggcca gttttcaatg tcgcggtacg ggtaatcttc aggcgtattt agcaacaata
     6901 ttttgatatt gttgtttttc ctgctcaaag tatcctgcca ataatggata agctttttat
     6961 ccgcttccat catatccaga agaataatag agcctgaaga gatatcgtcc agagaacgtt
     7021 gaatattatg taattttcct gtaattgcca gcgattgttt aaggtgctgc aagagagctg
     7081 tcgcctgcaa agaaggttta gtgatcaaca ataatgtatg accatgaata ctatggactt
     7141 cattaaacat gatgaaaccc cgcttttttt attgatcgca cacctgacag ctgcctctaa
     7201 aatagaagca ccagaagtac tgacagatgt tgcactgctg tgtgtagtaa taaatcagcc
     7261 ctaaatgggt aaaatataaa a
//
LOCUS       DAPHBJ010000210         7280 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.212, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000210 DAPHBJ010000000
VERSION     DAPHBJ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.212"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            148..390
                     /locus_tag="RHZ76_003931"
     CDS             148..390
                     /locus_tag="RHZ76_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814478.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(720..1649)
                     /gene="fdhE"
                     /locus_tag="RHZ76_003932"
     CDS             complement(720..1649)
                     /gene="fdhE"
                     /locus_tag="RHZ76_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HDV2814479.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKDPKIEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1646..2281)
                     /gene="fdoI"
                     /locus_tag="RHZ76_003933"
     CDS             complement(1646..2281)
                     /gene="fdoI"
                     /locus_tag="RHZ76_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDV2814480.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2278..3180)
                     /gene="fdoH"
                     /locus_tag="RHZ76_003934"
     CDS             complement(2278..3180)
                     /gene="fdoH"
                     /locus_tag="RHZ76_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HDV2814481.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3193..6243)
                     /gene="fdnG"
                     /locus_tag="RHZ76_003935"
     CDS             complement(3193..6243)
                     /gene="fdnG"
                     /locus_tag="RHZ76_003935"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5656..5658),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDV2814482.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSI
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPSSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6437..7270
                     /gene="fdhD"
                     /locus_tag="RHZ76_003936"
     CDS             6437..7270
                     /gene="fdhD"
                     /locus_tag="RHZ76_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDV2814483.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1628 a         1922 c         1966 g         1764 t
ORIGIN      
        1 ttgaacttat aataactgca actgttacat catatctgga aaacgcctcg caaaaataaa
       61 aaatgatgcg taaatgagct ttctttaaag attcggcaag ggtacttttc tctccataga
      121 ttcaaaaatg atattggcga gatatttatg aactctctgg caggtataga tatgggcaga
      181 attttactcg atttatcgaa tgaggtgatt aagcaacttg atgatctcga ggtgcagcgt
      241 aatcttcctc gtgcggacct attaagggaa gcggtagatc aatacctgat aaatcaatcg
      301 caaacagcaa gaaccagtgt tcctggcatc tggcaagggt gtgaggaaga tggtgtcgaa
      361 tatcagcgta agctgcgcga ggaatggtaa tgggaaaaaa tgatgtcaat cagattgctg
      421 acaacgtgcg cgttgttcat gccggatgcg gcgtgaacgc cttatccggc ctacaaagtc
      481 gtataaattc aatgtattgc agtttattgg taggcctgat aagcgcagcg catcaggcaa
      541 tcttacgctt gtcatcagtc tcatgcccat aaccttattg atgtggtggt gggtaaattg
      601 aggccaaacc ggcactggcg gcatagcttg aggtggtaac gcggaatacg agagatggtg
      661 tcgtcacacc ctatgagctg taatccgggc gacctgcgcc acccggaaaa cgtcagagat
      721 tactctcctt cacccggaaa caggaacggg ttgatggaac tgcgggcata gccttcttgc
      781 tccattcgtg cgtccagtac cagagaggcg aggtcatctg ctacggcttc gatttttgga
      841 tctttttcct gatagagaat tttcaggtaa gtgccgcagt catcgcagct ttcggcttta
      901 atcgcggcct gttcgtcatc cagcgaccag taatgcagtt tgccgctctg ctcgcagttg
      961 ctgcatttta cgcgcactac gtgccattca gtttcacaca ggttgcagtg caggtaacgt
     1021 agcccctgag tggtgccaat ttgcaccatg ctggacaccg gcatagaacc acaaaccggg
     1081 caatattgac gttgctcgcc gtattcagcg cgggctttgc cggggatcag attggccatc
     1141 tgcgcccagt agagcgacag tgcagcccag ataaacggcg ctttatcgct gctgacggac
     1201 gagaaatcag aggcaaacag cgcgctggcc atatcttcca gctcctgagt cgatgccttc
     1261 tccagattct caatcactgc cagcgccggg ccgctcattt caggtttcag ctcagcaatc
     1321 agcgccatca gcagcttttg ccagtgctta tcacgcggca gaacgtgaat atccagcggg
     1381 ggcttgcctt gtgcgctggc ttctttaatg cgcgcagtca gatccatctc cagcggatgg
     1441 tcgtacagca ccacttcctg ggcgtgggcg ataagcgcag caaagcgcag gtaatcaccc
     1501 agaggattat tttctgccag ctcgcgcaga cgctcggcgc ggcggttgta taaattcttg
     1561 agccgaggga acaataacgg cggaatcata tccgccgtac gtttctcgct tgaacccagc
     1621 tcatcttgcg ggattatgcg aatactcatt cagctttctt ttccgttgtc ttgcggacct
     1681 cacggtacca gcgcgggtga tgtttcttcg cccaggcgct ggtaacccat ccttccacca
     1741 tggcggtaat cgtgcctttc acccaaaggg cggcgtagat atgcaccatg ataaccacaa
     1801 ttaacgccac tgcggcaaat gaatgcagca ttaacgcgaa tcggatcacc gggattgaga
     1861 aagcaggcgc aaaataagga cgccagataa tcacgccgct caccagcagc agaaccagga
     1921 aaataatcgc cgcccagaaa acgcatttct gaccgaagtt ataacgcccg gtgtcaccta
     1981 cttcctcgtt gacgacgatc ttacgaatat tcttcgccca aaagatatca tcccgattga
     2041 ttaggttgtg atgccagtaa cggaaaaaca tgatgatgaa cgaggcaaac ataaccacgc
     2101 cgacaaacgg gtgcagaatt cgcgccagct gcggtgtgcc catgatttgc atcaaccagt
     2161 tgaaggacgg gaacaaaaag cccagcccgc tcaccgccgc caggatgaag cagaaggcgg
     2221 tgatccagtg gttgatacgt tccggcgcgg tgtagcgcac gatggtgtca cgtcgtttca
     2281 tttgcgctcc tcgtctttct cttcgtgcag attattctct tcctcatccg cacggttcgg
     2341 accgacaccc acgtagtgga agatactggc cgcaaaggta gccgcaaagc caacagccgc
     2401 gagcggtttc cagatgcctt tccagaattt cacggtttcg ctgatttccg ggttctccgg
     2461 caagccatga tacagatttg gcttgtcagc atggtgcagc acgtacatga cgtgtgtacc
     2521 accgacgcct gccggatcgt acagacccgc attgtcgtaa ccgcgggttt tcagctcagc
     2581 aacgcgctcg ctcgccagcg ttttcatcga ctctttcgta ccgaagtgaa tcgcgcccgt
     2641 tgggcaggtc ttcacgcagg ctggttcttg cccaaccacc acgcggtcaa cgcacagcgt
     2701 acatttgtag acgcggttgt cttccgggtt gaggcgcgga atgtcgaacg gacagcccgc
     2761 aatgcaataa ccgcagccaa tgcactgctc ggactggaag tcgacgatac cgttggcata
     2821 ctgaataatt gcaccttccg ccgggcacgc tttcaggcag cctggatcgg aacagtgcat
     2881 acagccgtct ttgcggatca gccattccag tttgtcgttc tgctccactt ccgagaagcg
     2941 cattaccgtc cacgatttgg cgcttaaatc attggggttg tcgtacaccc caatgttatt
     3001 gccgacggta tcgcgaatgt cgttccactc tgaacacgcc acctgacagg ctttacagcc
     3061 gatacaggtg gtaacgtcga tgagtttcgc cacttcttcc tggaagtccc gcgcctgagg
     3121 cgcgggggtc agaccgttag tcgcggaacg acgaatgata tcttgagatt gataagccat
     3181 atgtcgtctc cgttacacct tttccacatt cacaaggaag gacttaaact ccggcgtctg
     3241 cgtgttcgca tcaccaacga atggcgttaa agtattggca ataaagcctt ttttagctac
     3301 gccttcatag ccccagtgaa tcggaatacc gatggtatcg atatctttgc cgttcgcttt
     3361 cagcgtgcga atacgtttgg tcaccaccgc tttggctttg atatagccac ggttggagga
     3421 gactttcacg gtatcgccct gggcaatgcc aagtttattc gccagcgact ccccgatttc
     3481 cacaaactgc tctggctgca aaatcgcgtt caacagcgcg tgtttggtcc agtagtggaa
     3541 gtgctcggtc agacgatagg tggttccgac atacgggaac ttatcggctt tacccaatgc
     3601 ttcggcgtcg tctttaaaga tacgtgcagc cgggttcgag ataacgtttg gatgcagcgg
     3661 gttagttccc agcggcgttt caaacggctc gtagtgttcc gggaacggac cttccgccat
     3721 cttatcgagg gcaaacagac gccccatgcc ttcctgctgc atgataaacg gcccgacgcc
     3781 gctacccgga ggcgctgcgc tgtagtccgg aatatcccag ccggtccact tagtgccgtc
     3841 ccatttcagc aactgacgct tcggatccca cgggttaccc tgcggatctg cggaggcgcg
     3901 gttatacaga atgcggcggt taagcggcca tgcccatgcc cagcccagcg tgttaccgag
     3961 gccagacgga tcggcgttat cacggcgtgc catctggttg ccttccggcg tccagctacc
     4021 ggcgaaaatc cagcagccac aggaggttgt accgtcatcg cgcaattggg cgaacgaact
     4081 aagttgttgg cctttcttga cgataaccgc accggttgcc ggatcggtaa tatcggccag
     4141 cgccttaccg ttgctctcca tcgccacttc ttctgaagat ggctcatgcg gaatggcgta
     4201 gttccatgtc atgttcagca cctggtccgg gttcgcgcca ccctgttcgg catacatctt
     4261 gcgcaagcgc aggaagatac cggagaggat ctcgccgtca gtcagcgcaa tccccggggc
     4321 gtccgcacct ttccagtgcc actgtaacca gcgacctgag ttaacgatag aaccgttctc
     4381 ttccgcgaag caggtcgatg gcagacggaa cacttcggtc tggatcttcg acgagtcaac
     4441 ttcgttcagc tcaccgtggt tctgccagaa gttagaggtt tcagtgttca gtgggtcgat
     4501 ggtgacgagg aacttcagtt tcgacagaca accgatcact ttgtttttgt tcgggaatga
     4561 ggcaacaggg ttaaagccct ggcagatata gccattgacc ttgccctctt tcatcatctc
     4621 gaagtattgc aggacgtcgt agcctttatc ccacttcggc aaccagtcaa agccccagct
     4681 attttccgcc gtcgctttat caccaaagaa ggccttcatt atagagacga agaatttcgg
     4741 gtagttgccc cagtagttta cctggccttc cagcagcggt tttggcgtgt tggcggtaag
     4801 gtaggtttgc agatcggtct gcttctcgct tggcagcgtc atgtaacctg gcaggctctg
     4861 cgacagcagc cccaggtccg tcagcccctg aatattggag tgaccgcgca gggcgttaac
     4921 gccgccgcct gccatcccca tattgccgag cagcagctgg atcatcgcca tcgtacgaat
     4981 gttttgcgca ccaacggagt gttgcgtcca gccgagggca tacaggaacg aggcagtttt
     5041 atcgtgagca ctggtttctg cgatgtattc gcagactttc aggaacgcgt cttttggcgt
     5101 accacagatg ttttcaacca catctggcgt gtagcgggaa acgtgctgtt tcagcaagtt
     5161 ccacacgcag cgcggatgtt gcagcgtggt atcgcgtttg gcgaagccgt tttcgtccag
     5221 ttcataagtc caggaggatt tatcgtactt gcgtttttcc gcgtcgtagc cggtgaacag
     5281 gccatcttca aagccgtaat cctcacgcac gatcaggctg gcgttggtat aggcttcggt
     5341 gtattcgcgg ttgaattttt cattgttcag caggtacagc aatacgcctg acaggaaagc
     5401 aatgtcagta ccggaacgaa taggggcata gtagtcagct accgccgccg tacgcgtaaa
     5461 gcgaggatcg atcacaatca gcttcgcgcc gttgtgaatt ttggcttcca tcgcccagcg
     5521 gaacccgacc gggtgagctt cagcggcgtt accgcccatc accacgacga ggttggcgtt
     5581 cttgatgtcg acccagtggt tggtcatcgc accgcgacca aatgttggag caagacttgc
     5641 taccgttggt ccgtgtcaga cacgcgcctg gttgtcgacc gcgagcatac ccagcgcgcg
     5701 ggagaatttt tgcgttaaat agccggtttc gttactcgac gcggaagcac acagcatccc
     5761 ggtggagagc cagcggttaa cagtcacgcc ttcggcgttt tgcgcaatgt agttagcatc
     5821 gcggtcttct ttcatcagtt tggcgatgcg atcaaacgcc tcttcccaac tgatttgctg
     5881 ccatttatca gaacctggcg cacggtattc cggaaacttc agacggcttt cggagtggat
     5941 gaaatccacc aggccagcgc ctttcggaca aagtgcaccg cggttgaccg ggtgatccgg
     6001 atcgccttcg atatggaaga tagatgcttt ggcgtttttt gctccgtcac cgaggctgta
     6061 catcaacagc ccacagccta cggaacaata ggtgcaggta ttacgggttt cgcgggtgcg
     6121 cagcagttta tactgccggg tttccgcgag tgctacgctg ggtgcaaaac ccagtgccgc
     6181 tgccgtggtg cctgccatac cgccagcgca gatcttaaag aactgccttc tgctgacctg
     6241 catggattgc tccttgtttc gacattgtca cgtcccattt acattcgctt gctgcgtgtg
     6301 cagggagtgg gagttatttt tctttgcgga aggggccgca aaggtccaga attggctcaa
     6361 tttccctcca tccaggaagg gtttgtaaca gaataccata atgttggtgt gtgtgttctt
     6421 atctggttaa gagaaagtga aaaaaacaca gcgaaaagaa atcgaaaatg tgacaaatat
     6481 cacaggtgtt cgtcaaattg agttatggcg gcgtgatgat ttacaacacc cacggttaga
     6541 tgaggtcgcg gaagaagttc ccgttgcgct ggtctacaac ggcatttcgc atgtggtgat
     6601 gatggcgtcg cccaaagacc ttgagtactt tgcgctcggt ttttcgcttt ccgaggggat
     6661 tatcgaaagt ccgcgcgata tcttcggcat ggatgtcgtt ccttcctgta atggtcttga
     6721 agtacaaatt gagctttcca gccgccgctt tatggggttg aaggagcgcc gccgggcgct
     6781 ggcgggacgt acgggctgtg gcgtatgcgg cgtggagcaa cttaatgaca tcggaaaacc
     6841 ggtgcagccg ctaccgttca cccagacgtt tgatctcaac aaactggatg atgcattacg
     6901 tcatctcaac gattttcagc cagtggggca actgactggt tgtactcacg ccgctgcctg
     6961 gatgttgcca tctggcgaac tggtcggcgg gcatgaagac gtgggtcgcc atgtggcgct
     7021 ggacaaactg ttaggccgcc ggtcacaaga aggggaaagc tggcagcaag gtgcggtact
     7081 ggtttccagt cgtgccagtt atgaaatggt gcaaaagtcg gcgatgtgcg gcgtagagat
     7141 tttgtttgcg gtgtctgccg cgaccacgct tgctgtagaa gtggctgagc gctgtaatct
     7201 gacactggta ggtttttgta aaccgggtcg ggcaacggtt tatacccatc cgcagcgttt
     7261 gagcaattaa ttaaaataag
//
LOCUS       DAPHBJ010000211         7261 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.213, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000211 DAPHBJ010000000
VERSION     DAPHBJ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.213"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            379..1416
                     /gene="dusA"
                     /locus_tag="RHZ76_003937"
     CDS             379..1416
                     /gene="dusA"
                     /locus_tag="RHZ76_003937"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HDV2814484.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDIDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            1550..1792
                     /gene="pspG"
                     /locus_tag="RHZ76_003938"
     CDS             1550..1792
                     /gene="pspG"
                     /locus_tag="RHZ76_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HDV2814485.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(1958..2941)
                     /locus_tag="RHZ76_003939"
     CDS             complement(1958..2941)
                     /locus_tag="RHZ76_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HDV2814486.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNII
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3024..4439
                     /gene="dnaB"
                     /locus_tag="RHZ76_003940"
     CDS             3024..4439
                     /gene="dnaB"
                     /locus_tag="RHZ76_003940"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDV2814487.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4492..5571
                     /gene="alr"
                     /locus_tag="RHZ76_003941"
     CDS             4492..5571
                     /gene="alr"
                     /locus_tag="RHZ76_003941"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDV2814488.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            5824..7017
                     /gene="tyrB"
                     /locus_tag="RHZ76_003942"
     CDS             5824..7017
                     /gene="tyrB"
                     /locus_tag="RHZ76_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HDV2814489.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLKQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVMCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGIYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1715 a         1774 c         1920 g         1852 t
ORIGIN      
        1 taaatcggag aaataatatg tgaagttctt cgatggtatg gaaggcatta cataaaagaa
       61 cccaatgctt attgggttct ttttttctct atcagtgcta ttagcaggga gagatatcac
      121 cggagtttaa tgtgtgattt ttatttatcg tcgaacctgg attgtttatc attggcctta
      181 acaaagctaa cggctaataa gattatttcc atcacttcgt gagagcttca tgccttgaga
      241 ggatctcaat tttcttttgc aatgagacag gcgcttcctg ttgttatggt atagtacccc
      301 gttattgagc ctcctgaaca gtgatgctga ataacataac cccatgatat atcgataaaa
      361 taatctctac atttgaaaat gcacggtaat tctgaaatgc aaaaaatcaa ccaaaccagc
      421 gcaatgcctg aaaaaactga cgttcactgg agtggtcggt ttagcgttgc accaatgctc
      481 gactggacgg acagacattg ccgctatttc ttgcgtctgc tttcccgcaa tacgttgctg
      541 tataccgaaa tggtgaccac aggggcgatt attcacggta aaggtgatta cctggcgtac
      601 agtgaagaag aacatccggt agcgttgcaa ctcggcggta gcgatccggc ggcgctggca
      661 cagtgtgcga agctggcaga agcgcgtgga tatgatgaga tcaacctgaa tgtcggctgc
      721 ccgtctgacc gggtgcagaa cggcatgttt ggtgcgtgtc tgatgggtaa tgcgcagctg
      781 gttgccgact gcgtgaaagc gatgcgcgat gtggtgtcga ttccggtgac ggtgaaaacg
      841 cgtattggca tcgacgacca ggacagctat gaatttctct gcgatttcat caacaccgtt
      901 tccggcaaag gcgagtgtga gatgttcatc atccatgcac gtaaagcctg gctttcgggg
      961 ttaagtccga aagaaaaccg tgagatcccg ccgctcgatt atccgcgtgt gtatcaactg
     1021 aagcgtgact ttccgcatct gacaatgtcg attaacggtg gtatcaagtc gctggaagag
     1081 gccaaagcac acctgcaaca tatggatggc gtgatggtcg ggcgcgaggc gtatcagaat
     1141 ccgggtattc tggcggcggt agaccgggag atctttggtt cctcggatat cgatgccgat
     1201 ccggtggcgg tagtgcgcgc catgtatccg tacattgagc gtgaactcag ccaggggacg
     1261 tatctcggtc atattacccg gcatatgttg ggcttgttcc agggtattcc tggcgcgcgg
     1321 cagtggcggc gttatttaag tgaaaatgcc cataaagcgg gtgcagacat taatgtgctg
     1381 gaacacgcgc tcaaactggt ggcggataag cgttaacttt tcaccaaaaa atagtcaaat
     1441 tcaccacgcc ctgcgcaccg tcgcggggcg ttttgctgtt aaatcaatag attatttttg
     1501 gcatgattct tgtaatgcca gcaagagatt tcatatttgg gagtgcatca tgctggaact
     1561 actttttgtg attggctttt ttgtcatgct gatggtcacc ggcgtttcgt tgctgggcat
     1621 tatcgccgcg ctggttgtgg cgacggccat tatgttcctc ggcggtatgc tggcattgat
     1681 gattaagttg ctgccgtggt tactactggc gattgcggtg gtgtgggtta ttaaggcgat
     1741 taaagcacca aaagtgccga aatatcagcg ttatgaccgc tggcgttact aagggattgt
     1801 gcggatgatc acaacctaag gttttatcct tagaacaaaa taggaattga taatcaaatc
     1861 tgtcactatt gcgcctctaa cagattcatc gtgctgtacc ctacatacag ccgaactata
     1921 aaaagaaagg gcttcccagg tggaagccct atttctttta tggaatcaac aggctggaac
     1981 cttgcgttgc ccggctttcc agaatttcat gcgcacgctg cgcatccttc agcggatatt
     2041 tctgctgctc ggcgacatcg accttaatca caccgctggc aatcaaagag aacagttcat
     2101 tactggcctc ggttaattcc tcccgcgtgg tgatatagcc ttgcagggaa gggcgtgtca
     2161 catacaacga gcctttctga ttgagaatgc ctaagttcac accggtaacc gcacctgatg
     2221 agttgccaaa actcaccatt aagccgcggc gttgcaggca atccagcgac cgttcccagg
     2281 tgtctctgcc cacggaatcg tacaccacgc gcactttctt accgccggtg atctctttta
     2341 accgctcgac cagattctct tcacgatagt taataacctg ccacgcgccc gcttttagcg
     2401 cgctctgcgc tttttgcgcg gttcctacgg tgccgataag tttcgcgccc agggcttttg
     2461 cccactggca ggcaatcaag ccaacgccgc cagctgctgc gtggaacagg aactgctcat
     2521 cgggtttaat ttcataggtt ttgcgcagca gataataaac cgttaagcct ttcaggaagg
     2581 atgccgcagc ttgctcaaaa gaaattgccg caggcagaat cgccgcttta tccgcaataa
     2641 tgttatgcac agagctgtaa gcgcctaacg ccgactgcgc atagactaca cgatcgcctg
     2701 ccttaatatg ctttacacca ctgccgactt tactcacgat gcctgctgct tcggtgccta
     2761 atccgctggg tagcgatggc ggcgggtaaa ggccgctgcg gatatatgtg tcgataaaat
     2821 tgatgccgat ggctttattt tcgacctgga tttcattctc cgccggatcg gcaggagtga
     2881 actctacggc ttgaagtact tccgggccac cgtgcttgtg aaattcaatt cgtgttgcca
     2941 tgtgtcctcc agaacgtatc gtcagggtct gcttcatatg gtaaagtttc gacccattct
     3001 ttatctcggt aactccattc actatggcag gaaataaacc cttcaacaaa cagcaggctg
     3061 aaccccgcga acgcgatcca caagttgccg ggctgaaagt gcctccgcac tcgatcgaag
     3121 cggagcagtc ggtgttgggc ggtttaatgc tagataacga acgctgggat gatgtagccg
     3181 agcgtgtggt agcagacgat ttttacaccc gcccacaccg tcatatcttt actgaaatgg
     3241 cgcgtttgca ggaaagcggt agccctatcg atctaattac tcttgcggaa tcgctggaac
     3301 gccaggggca actcgatagc gtcggtggtt ttgcttatct ggcagagctg tcaaaaaata
     3361 cgccaagtgc ggcgaacatc agtgcttatg ctgacatcgt gcgtgaacgt gccgttgtcc
     3421 gtgagatgat ctcggttgcg aatgagattg ctgaagccgg ttttgatccg caggggcgta
     3481 ccagcgaaga tctgctggac cttgctgaat cccgcgtctt taaaattgcc gaaagtcgtg
     3541 caaacaaaga cgaagggccg aagaacatcg ccgatgtgct cgacgcaacc gtggcgcgta
     3601 ttgagcagtt gtttcagcag ccacacgatg gcgttaccgg agtaaacacc ggttatgacg
     3661 atctcaacaa aaaaaccgct ggcttgcagc cgtcggattt gatcatcgtc gccgcgcgtc
     3721 cgtcgatggg taaaacaaca tttgcgatga acctcgtcga aaacgcggcg atgttgcagg
     3781 ataaaccagt acttatcttc tcgctggaga tgccttcaga acagattatg atgcgttctc
     3841 tggcgtcgct gtcgcgcgtt gaccagacta aaatccgtac cgggcagctc gatgatgaag
     3901 actgggcgcg catttccggc accatgggta ttttgctcga aaaacgcaat atctatatcg
     3961 atgactcctc cggcctgacg ccaacggaag tgcgttcccg cgcacgccgt attgcccgtg
     4021 aacacggcgg catcgggctt atcatgatcg actacctgca actgatgcgc gtaccggcgc
     4081 tttccgataa ccgtacgctg gaaattgcag aaatctctcg ctcgctgaaa gcactggcga
     4141 aagaactgaa cgtgccggtg gtggcgctgt cccagttgaa ccgttctctg gaacaacgtg
     4201 ccgacaaacg cccggtcaac tccgacctgc gtgaatctgg ctctatcgag caggatgcgg
     4261 acttgatcat gtttatctat cgtgatgagg tgtatcacga aaacagtgat ttaaaaggca
     4321 tcgcggaaat tattatcggt aaacaacgta acggcccaat cgggacggta cgcctgacct
     4381 ttaacggtca atggtcgcgc ttcgacaact atgcggggcc gcagtacgac gacgaataat
     4441 aattatttta tgaattaggt aattaaagca aacacttatc aaggaacaca aatgcaagcg
     4501 gcaactgttg tgattaaccg ccgcgctctg cgacacaacc tgcaacgtct tcgtgaactg
     4561 gcccctgcca gtaaaatggt tgcggtggtg aaagcgaacg cttatggtca cggtcttctt
     4621 gagaccgcgc gaacgctccc cgatgctgac gcctttggcg tagcccgtct cgaagaagct
     4681 ctgcgactgc gtgcgggggg aatcaccaaa cctgtactgt tactcgaagg cttttttgat
     4741 gccagagatc tgccgacgat ttctgcgcaa cattttcata ccgccgtgca taacgaagaa
     4801 cagctggctg cgctggaaga ggctagcctg gacgagccgg ttaccgtctg gatgaaactc
     4861 gataccggta tgcaccgtct gggcgtaagg ccggaacagg ctgaggcgtt ttatcatcgc
     4921 ctgacccagt gcaaaaacgt tcgtcagccg gtgaatatcg tcagccattt tgcgcgcgcg
     4981 gatgaaccaa aatgtggcgc aaccgagaaa caactcgcta tctttaatac cttttgcgaa
     5041 ggcaaacctg gtcaacgttc cattgccgca tcgggtggca ttctgctgtg gccacagtcg
     5101 cattttgact gggtgcgccc gggcatcatt ctttatggcg tctcgccgct ggaagatcgc
     5161 tccaccggtg ccgattttgg ctgtcagcca gtgatgtcac taacctccag cctgattgcc
     5221 gtgcgtgagc ataaagccgg agaacctgtt ggttatggtg gaacctgggt aagcgaacgt
     5281 gatacccgtc ttggcgtagt cgcgatgggc tatggcgatg gttatccgcg cgccgcgccg
     5341 tccggtacgc cagtgctggt gaacggtcgc gaagtaccga ttgtcgggcg cgtggcgatg
     5401 gatatgatct gcgtagactt aggtccacag gcgcaggaca aagccgggga tccggtcatt
     5461 ttatggggcg aaggtttgcc cgtagaacgt atcgctgaaa tgacgaaagt aagcgcttac
     5521 gaacttatta cgcgcctgac ttcaagggtc gcgatgaaat acgtggatta atcgttctgt
     5581 aatatttgat tgtctgtgcc ggatgcggcg tgaatgcctt atccggccaa taaaatccta
     5641 aaaattcaat aagttgatgt tctttcatgc tcttataaag gtcgtgcctc tggcggatgt
     5701 acgtttgtca tgagtctcac tctgttgcta attgccgttc gctcctgaac atccactcga
     5761 tcttcgcctt cttccggttt attgtgtttt aaccacctgc ccgtaaacct ggagaaccat
     5821 cgcgtgtttc aaaaagttga cgcctacgct ggcgacccga ttcttacgct tatggagcgt
     5881 tttaaagaag accctcgcag cgacaaagtg aatttaagta tcggtctgta ctacaacgaa
     5941 gacggaatta ttccacaact gcaagccgtg gcggaggcgg aagcgcgcct gaatgcgcag
     6001 cctcatggcg cttcgcttta tttaccgatg gaagggctta acagctatcg ccatgccatt
     6061 gcgccgctgc tgtttggtgc ggaccatccg gtactgaaac aacagcgcgt agcaaccatt
     6121 caaacccttg gcggctccgg ggcattgaaa gtgggcgcgg atttcctgaa acgctacttc
     6181 ccggaatcag gcgtctgggt cagcgatcct acctgggaaa accacgtagc aatattcgcc
     6241 ggggctggat tcgaagtgag tacttacccc tggtatgacg aagcgactaa cggcgtgcgc
     6301 tttaatgacc tgttggcgac gctgaaaaca ttacctgccc gcagtattgt gttgctgcat
     6361 ccatgttgcc acaacccaac gggtgccgat ctcactaatg atcagtggga tgcggtgatt
     6421 gaaattctca aagcccgcga gcttattcca ttcctcgata ttgcctatca aggatttggt
     6481 gccggtatgg aagaggatgc ctacgctatt cgcgccattg ccagcgctgg attacccgct
     6541 ctggtgagca attcgttctc gaaaattttc tccctttacg gcgagcgcgt cggcggactt
     6601 tctgttatgt gtgaagatgc cgaagccgct ggccgcgtac tggggcaatt gaaagcaaca
     6661 gttcgccgca actactccag cccgccgaat tttggtgcgc aggtggtggc tgcagtgctg
     6721 aatgacgagg cattgaaagc cagctggctg gcggaagtag aagagatgcg tactcgcatt
     6781 ctggcaatgc gtcaggaatt ggtgaaggta ttaagcacag agatgccaga acgcaatttc
     6841 gattatctgc ttaatcagcg cggcatgttc agttataccg gtttaagtgc cgctcaggtt
     6901 gaccgactac gtgaagaatt tggtatctat ctcatcgcca gcggtcgcat gtgtgtcgcc
     6961 gggttaaata cggcaaatgt acaacgtgtg gcaaaggcgt ttgctgcggt gatgtaatgc
     7021 aggaaagcag gctggagtta cccagcctgc agtgaaatta aactgtcgtc gctttcacta
     7081 tttctttgta gatgattttt ttgatgccgt tgttctacgt gagagataat aaaacgttgt
     7141 cagttctttt attgttaagt ttatcccaat tatctggaat tcctttccct attttttcgt
     7201 gtggagtaat aatttctgca catctaacca ttattaaatt taataataag tatcgggttt
     7261 g
//
LOCUS       DAPHBJ010000212         7179 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.214, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000212 DAPHBJ010000000
VERSION     DAPHBJ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.214"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..3029)
                     /gene="rhsB"
                     /locus_tag="RHZ76_003943"
     CDS             complement(<1..3029)
                     /gene="rhsB"
                     /locus_tag="RHZ76_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HDV2814490.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWVLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHHEAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQASSGGTEPSAFPDT
                     LPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVR
                     SFTYDDKYRGRMVAHRHTSRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDHITITDS
                     LDRREVLHTQGEAGLKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVT
                     GLITRITTPDGRASAFYYNHHNQLTSATGPDGLELRREYDELGRLIQETAPDGDITRY
                     RYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHRE
                     EGLSQYRAYDSRGQLIAVKDTQGHETRYEYNIAGDLTAVIAPDGSRNGTQYDAWGKAV
                     RTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLT
                     GKLIRSEDEGLVTHWHYDEADRLTHRTVNGETAERWQYDERGWLTDISHISEGHRVAV
                     YYGYDEKGRLTGERQTVHHPETEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYG
                     SGYLAGMKLGDTPLVEYTRDRLHRETLRSFGRYELTTAYTPAGQLQSQHLNSLLSDRD
                     YTWNDNGELIRISSPRQTRSYSYSTTGRLTGVHTTAANLDIRIPYATDPAGNRLPDPE
                     LHPDSTLSMWPDNRIARDAHYLYRYDRHGRLTEKTDLIPEGVIRTDDERTHRYHYDSQ
                     HRLVHYTRTQYEEPLVESRYLYDPLGRRVAKRVWRRER"
     gene            3257..3865
                     /locus_tag="RHZ76_003944"
     CDS             3257..3865
                     /locus_tag="RHZ76_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDV2814491.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            3963..5354
                     /gene="selA"
                     /locus_tag="RHZ76_003945"
     CDS             3963..5354
                     /gene="selA"
                     /locus_tag="RHZ76_003945"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDV2814492.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASRKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            5351..>7179
                     /gene="selB"
                     /locus_tag="RHZ76_003946"
     CDS             5351..>7179
                     /gene="selB"
                     /locus_tag="RHZ76_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDV2814493.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDAL"
BASE COUNT         1413 a         1998 c         2156 g         1612 t
ORIGIN      
        1 cgttcacgcc gccacacccg ttttgccacc ctgcggccca gcgggtcgta aagatagcga
       61 ctttcgacca gcggctcttc atattgtgtc cgcgtgtagt gcaccagccg gtgctgactg
      121 tcgtaatggt accggtgagt ccgctcatca tccgtgcgga taaccccttc cgggatgagg
      181 tcggttttct ctgtcagcct gccgtgacgg tcataccggt aaagatagtg cgcgtcacgg
      241 gcgatacggt tatccggcca catgctgagg gtgctgtccg ggtgcagctc cgggtcgggc
      301 aggcggttac ctgccgggtc tgtggcatac gggatgcgga tatccagatt cgctgcggtg
      361 gtgtgaacgc cggtcagcct gccggtggtg ctgtagctgt aactccgggt ctggcgcggg
      421 ctgctgatgc ggatgagttc gccgttgtcg ttccaggtgt aatcgcggtc agacagcagg
      481 ctgttcaggt gctggctctg taactgcccg gcaggggtat aagcggtggt gagttcataa
      541 cggccgaagc tgcgcagcgt ttcccggtgc aggcggtcgc gggtgtactc caccagcggt
      601 gtgtcgccga gtttcatgcc tgccaggtaa ccgctgccgt aggtcagcca ttccacggcg
      661 ggcaggctgt ccggtataca gcggttcgcc agcccctgcg cgttgtacgc atgtctggtc
      721 tcatgctgcc agagcagtgc ttccgtctcc gggtgatgca ccgtctgacg ctcaccggtc
      781 aggcggcctt tctcatcata cccgtaatac accgccaccc ggtgcccttc gctgatatgg
      841 ctgatgtctg tcagccagcc gcgttcgtca tactgccacc gctctgcggt ttcgccattc
      901 acggtgcggt gcgtgaggcg gtctgcttcg tcatagtgcc agtgggtgac cagcccctca
      961 tcctcgctgc ggataagttt gccggtcagg tcgtggtgat aacgctgtgt gcggccgtca
     1021 aagccggttt cctgtatcag ccggtcaagt acatcgtaac ggaaggtggt gtggctgccg
     1081 ttttcactgg tcaggcggat gacccgtccg gcagcatcgt attccatact gcgcgtcagc
     1141 ccgccctgcg tggtacggac ggcctttccc cacgcatcgt actgtgtccc gtttctgctg
     1201 ccgtccgggg caatgacggc ggtcaggtca ccggcgatgt tgtattcata ccgcgtttca
     1261 tggccctgcg tgtctttcac ggcaattaac tgtccacggc tgtcgtatgc gcggtactga
     1321 ctcagccctt cctcgcggtg caccgccgtc atctgcccga agcggtcatg gtcataacgg
     1381 gttacataac cggaacagtc ggtgaagctc agcaactgac cgtaacggct ccacgtcatg
     1441 gttttccggc tgccggtggc atcttccgtt gcgcagggta agtcactgtg tggattatca
     1501 taacggtagc gggtgatatc gccgtcaggg gcagtttcct gaatcagacg gcccaattca
     1561 tcatattccc ggcgcaattc cagcccgtca ggcccggtgg ctgacgttaa ctggttgtgg
     1621 tggttatagt aaaacgccga tgccctgcca tccggcgtgg ttatgcgcgt gatgaggccc
     1681 gtcaccacat ccgggctgta ttctgttgtc cggcctgcgg catccgtctg tgccctgagc
     1741 ctgcccacgg cgtcaaactg actctgcgtg acgctgccgt ccgcgtgttc ctttttcacc
     1801 acccgcttca gcccggcttc gccctgcgtg tgcagcactt cacggcggtc caggctgtcg
     1861 gtgatggtga tgtggtcttt ctcatactga tacgtgtagc ttaagcctgc cgggtttagc
     1921 tgttctgtca cccgcccgtc gctgtcgtaa cggtaacgga tttccggtcg gctcgtgtga
     1981 cggtgcgcca ccatccggcc ccggtattta tcatcgtaag taaagctgcg cacctgtgta
     2041 ttgctgcggt catacactgc cgccagttcg ccgcgcggcg tccagccata gcgcaccagc
     2101 ggcgcggcag gtaaattctc cgggtattcc gggtcgtgcg tcagccacac ggcagacaga
     2161 cggatgccgt tgtcccggcc atattcggtg taacccggca gggtatcagg aaaagcggac
     2221 ggttccgtcc cgccggaaga ggcctgctgc cgggcttctt ctgcccgctg cgcctgcgtg
     2281 gtcagtacca gccggaagtg acgcccggca ccatccgtca cgccggtgat ttcgccgctg
     2341 aattcaccgg cggcttcgtg gtggaacgtc tgtgtgcgcc cgaagcggtc caccagcccg
     2401 gtcagtaccc ggtacggcgg cagcggcgca ggcagcacct catccgcttc cggcacccgc
     2461 tcacaccagc cgagtaccca ccacggcccc tgcggactgt ttgtcgccag ataacgatgc
     2521 ggactcaggc gaagctcttc cggcagcgcc tgccagagtg cggccagccg gtgaccttca
     2581 tccagtttcg ccacaccgcc gcgcaccagc cagagtgatt cgctgcggct gtaaccgtcc
     2641 tcaccgggaa acaggtgctc aaaatacagg cttctgccgc cgttatcact gagtatcagt
     2701 gtgttatcgc gcagctgtaa gcggatatcc gcaggcattt tccagccggg gccgaggctc
     2761 cccaccggcg cgggcgtttt tgtccggtaa ctgctgtagg tgcgggagag aatgaacggc
     2821 agcgggccgg gcagggcgat gtcggtttca ccgggaagga cctttgcacc cagcagcgga
     2881 ttgaccggat ggccggacgt caccccgccg gggcacaccg aacaggccac gccggtgggg
     2941 gcaccaatgc gcaccccggc tgaaccctga acaatgctac cgccatactg cgtcatgtcg
     3001 ccctgacgcg ctgccggttt tccgctcatt tctcatcctt gttttatcgg gttatttgtc
     3061 tgctgacagg cgaaacccta cagatttata agaaagcctg aagtgagata tccatcagag
     3121 tgaaataatt aggaaaatat ttatatcacc atgataaata tgtgatttat ttggaagtaa
     3181 caaaccatca acattttctg ccctttttcc catcaccagg ccatactgat atttttcctc
     3241 gcacaaagga ctcggcatga aactcgtcgg tagctacacc agcccgtttg tacgcaaact
     3301 ttctattctg ttgttagaaa agggcataac tttcgaattt attaatgaac tgccctataa
     3361 cgcggacaac ggcgtggcgc aatttaaccc gttaggaaaa gtgccggtgc tggtgaccga
     3421 agagggcgaa tgttggtttg attcgccgat catcgctgaa tatattgaat taatgaatgt
     3481 cgctccggcg atgttgccgc gcgatccgct ggagtcgttg cgggtgcgca aaattgaggc
     3541 actggcggat ggcattatgg atgccgggct ggtatcggtg cgtgaacagg cgcgtccagc
     3601 ggcgcagcag tctgaagatg aattgttacg ccagcgggag aaaatcaacc gcagtctgga
     3661 tgtgctggaa ggatatctgg tcgatggcac actcaaaacc gatacggtca atctggcgac
     3721 tatcgccatt gcctgtgccg tcggatatct caatttccgc cgcgttgcgc caggttggtg
     3781 tgtcgaccgc ccacacttag tcaaactggt cgaaaacctg tttagccgcg aaagttttgc
     3841 tcgcaccgaa ccgccaaagg cttgatgcgc gatatgtcct cctgacccat ctcacgttac
     3901 aatccgtggt tatgttaaac gcccttctcc gtgtgagagg gccttgatca gccaggtttc
     3961 ctatgacaac cgaaacgcgt tccctctata gtcaacttcc ggctattgat cgcttattgc
     4021 gcgatagctc ctttctttct ttgcgtgata cttatggtca cacccgcgtg gtggaattgt
     4081 tgcgtcagat gctcgacgaa gcgcgagaag tgattcgtgg cagccagacg ctgcctgcgt
     4141 ggtgtgaaaa ctgggcgcaa gaagtcgatg cccggttgac gaaagaagcg cagagcgcgc
     4201 tgcgtccggt gatcaacctg acgggaaccg tgctgcatac caaccttggg cgagctttac
     4261 aggcggaagc cgcggtggaa gccgttgcgc aggctatgcg ttcgccagtg accctcgagt
     4321 acgatctgga cgacgccgga cgcggacatc gcgatcgggc gctggcgcag ctgctgtgcc
     4381 gtattacggg ggcggaagat gcctgtatcg tcaataacaa tgcggcggcg gtgttattga
     4441 tgttggcggc cactgccagc agaaaagagg tggtggtatc tcgcggcgaa ctggtggaga
     4501 ttggcggcgc gtttcgtatt cccgatgtta tgcgtcaggc aggctgcacc ctacacgaag
     4561 tagggaccac caaccgcacg cacgcgaatg attatcgtca ggcggtgaat gaaaataccg
     4621 cactgttgat gaaagtacat accagtaact acagcattca ggggttcacc aaagcgatag
     4681 atgaagcgga actggtggcg ctcggcaaag agctggatgt tcccgtagtg actgatttag
     4741 gcagtggctc gctggtcgat cttagccagt acggtttgcc gaaagagcca atgccgcagg
     4801 agttgattgc ggcgggcgtc agtctggtga gtttctccgg cgacaagttg ttaggcgggc
     4861 cgcaggcagg aattattgtt ggtaaaaaag agatgatcgc ccgcctgcaa agccacccgc
     4921 tgaagcgtgc attacgcgcg gataaaatga ccctcgcggc gctggaagcc acgttgcgtc
     4981 tttatttaca ccctgaagct ctgagtgaaa aattaccgac cctgcgcctg cttacccgca
     5041 gcgcagaggt cattcaaatc caggcacaac gtttacaggc cccccttgcc gcacattacg
     5101 gcgcggagtt tgcggtacag gttatgccat gtctttcgca gattggcagt ggttcgctgc
     5161 cggttgatcg cctgccgagc gcggcattaa cgtttacacc ccatgatgga cgcggtagcc
     5221 accttgagtc attagccgcc cgctggcgtg aattgccagt gccggtgatt ggtcgtattt
     5281 atgacggacg attgtggctg gatttacgct gccttgaaga tgagcaacgg tttttggaga
     5341 tgttgttgaa atgattattg cgactgccgg acacgttgac cacggcaaaa caaccttatt
     5401 gcaggcgatt actggcgtaa atgctgaccg tctgccggaa gaaaaaaagc gcggcatgac
     5461 catcgatctc ggctatgcct actggccgca gccggatggt cgcgtgcctg gttttatcga
     5521 cgtacccggt catgaaaagt ttctttccaa catgctggcg ggcgttggtg gtatcgatca
     5581 cgcgctgttg gtggtggcgt gcgatgacgg cgtgatggca cagacccgtg agcatctggc
     5641 gattttgcag ctgaccggta acccgatgct gacagtggcg ctgaccaaag ctgatcgcgt
     5701 ggacgaagcg cgtgttgatg aggttgaacg ccaggtaaag gaggttctgc gggaatacgg
     5761 ttttgctgag gcaaaactgt ttatcaccgc agcaaccgaa ggtcggggaa ttgatgccct
     5821 gcgcgagcat ctgcttcagt taccaggacg tgaacacgcc agccaacata gtttccgcct
     5881 cgcgatcgac cgcgcattta ccgtaaaagg tgccgggctg gtcgtcaccg gtacggcgtt
     5941 aagcggggaa gtgaaggtag gcgattcact ctggctgact ggtgtaaata aaccgatgcg
     6001 tgtacgtgcg ctgcatgcac aaaaccagcc aacagaaacc gcccatgccg ggcagcgtat
     6061 cgcgcttaac atcgcgggtg atgcggaaaa agagcagatt aaccgtggcg actggctgct
     6121 tgccgatgcg ccgccagagc cgttcacacg ggtgattgtc gagcttcaaa cccatacgcc
     6181 gctgacccag tggcagccgc tgcatattca ccacgccgcc agccacgtta cgggacgcgt
     6241 ttcgctgctg gaagataacc ttgccgagct ggtgttcgac acgccgctgt ggctggcgga
     6301 taacgatcgc ttagtgctgc gcgatatctc ggcccgcaac acgctggcgg gggcgcgtgt
     6361 ggtgatgctt aacccgccgc gtcgcggtaa acgtaagccg gaatatctgc aatggctggc
     6421 gtctcttgca cgggcgcaga gcgatgccga tgcgttatct gttcatctgg aacgcggcgc
     6481 ggttaacctt gcggatttcg cctgggcgcg ccagctcaac ggcgaaggga tgcgcgaatt
     6541 gctgcaacag cctggttata ttcaggctgg ttatagcttg ttgaatgcgc cagttgccgc
     6601 ccgctggcag cggaaaattc tcgacacatt agcgacttat catgagcaac atcgcgatga
     6661 acctggtcct gggcgcgaac gtctgcgacg tatggcgttg ccaatggaag atgaagcgct
     6721 ggtactgttg ctgattgaaa agatgcgcga aagcggcgac atcctcagtc atcacggttg
     6781 gctgcatctg ccagatcaca aagcgggctt cagcgaagag cagcaggcca tctggcaaaa
     6841 agcagagccg ctgtttggtg acgagccgtg gtgggtgcgt gacctggcaa aagagacggg
     6901 aaccgacgag caggcaatgc gcctgacgct gcgccaggcg gcgcagcaag gaataattac
     6961 cgcgatcgtc aaagatcgtt attaccgtaa cgatcggatt gtcgaatttg ccaatatgat
     7021 ccgcgatctc gatcaggagt gtggttcaac ctgcgcggcg gatttccgcg atcgcttagg
     7081 cgtaggccga aagctggcaa ttcagattct ggaatatttt gaccgcattg gctttacgcg
     7141 tcgtcgtgga aatgatcatt tattgcgcga cgcattatt
//
LOCUS       DAPHBJ010000213         7174 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.215, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000213 DAPHBJ010000000
VERSION     DAPHBJ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.215"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..206)
                     /locus_tag="RHZ76_003947"
     CDS             complement(<1..206)
                     /locus_tag="RHZ76_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HDV2814494.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTTGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRAT"
     gene            474..908
                     /gene="uspF"
                     /locus_tag="RHZ76_003948"
     CDS             474..908
                     /gene="uspF"
                     /locus_tag="RHZ76_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HDV2814495.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEEEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1085..2020
                     /gene="ttcA"
                     /locus_tag="RHZ76_003949"
     CDS             1085..2020
                     /gene="ttcA"
                     /locus_tag="RHZ76_003949"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDV2814496.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2149..3522)
                     /gene="dbpA"
                     /locus_tag="RHZ76_003950"
     CDS             complement(2149..3522)
                     /gene="dbpA"
                     /locus_tag="RHZ76_003950"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HDV2814497.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTTAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIATLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(3999..4982)
                     /gene="zntB"
                     /locus_tag="RHZ76_003951"
     CDS             complement(3999..4982)
                     /gene="zntB"
                     /locus_tag="RHZ76_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HDV2814498.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            5237..6469
                     /gene="dgcM"
                     /locus_tag="RHZ76_003952"
     CDS             5237..6469
                     /gene="dgcM"
                     /locus_tag="RHZ76_003952"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HDV2814499.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAAKLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRKLTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(6490..7053)
                     /gene="smrA"
                     /locus_tag="RHZ76_003953"
     CDS             complement(6490..7053)
                     /gene="smrA"
                     /locus_tag="RHZ76_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HDV2814500.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
BASE COUNT         1872 a         1835 c         1700 g         1767 t
ORIGIN      
        1 gttgcccggc atccaacgcc attcatggcc atatcaatga ttttctggtg cgtaccgggt
       61 tgagaagcgg tgtaagtgaa ctgcagttgc catgttttac ggcagtgaga gcagagatag
      121 cgctgatgtc cggtggtgct tttgccgtta cgcaccacgc cttcagtagc ggagcaggaa
      181 ggacatctga tggaaatgga agccacgcaa gcaccttaaa atcaccatca tacactaaat
      241 cagtaagttg gcagcattac cgacagcttc tacgctgcca atggcatctc taccgatgat
      301 attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt tatggcgggc
      361 tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc gtcattacac
      421 aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt tttatgaaca
      481 gaacgattct tgtccctatc gatatttccg attcagaatt aactcaacgc gtgattagcc
      541 acgttgagga agaggcaaag attgatgatg cagaggttca tttcctgacg gtaatacctt
      601 cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg gcaatggatg
      661 acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt aaactgccaa
      721 ccgacagagt gcatgtccat gttgaggaag gctcgcccaa agaccgcatt ctggaattgg
      781 cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat atcaccactt
      841 atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc gtgctggttg
      901 tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc gcaaatgtgc
      961 tgacattttc ccctctaatc cgtaccatac acgccacagt tttttatatc agatttctta
     1021 tgctgggcgt tccggcatga cgcatactct tctgatgcca tataacgaat tgagtcgctt
     1081 ttaaatgtcg caaaatcaag aaattagtaa gaaagaacaa tacaacctga acaaattaca
     1141 aaaacgtctg cgtcgtaacg tgggcgaagc cattgctgac ttcaatatga ttgaagaagg
     1201 cgatcgcatc atggtttgcc tctccggggg taaagacagc tataccatgc tggagattct
     1261 gcgcaatttg cagcaaagcg cgccaatcaa tttttcgctg gtggctgtta acctcgatca
     1321 aaagcaaccg ggcttcccgg aacacgttct gcccgagtat cttgaaaagc tgggcgttga
     1381 gtacaagatt gttgaagaga atacttacgg tatcgtgaaa gagaagattc cagagggcaa
     1441 aaccacttgc tcactgtgtt ctcgccttcg tcgcggtatc ctttatcgta ccgcaacgga
     1501 actgggggcg acgaagatcg cgttgggtca ccatcgtgac gatatcctgc aaacgttgtt
     1561 cttaaatatg ttctacggcg gtaagatgaa aggtatgcct ccgaaactga tgagcgatga
     1621 tggcaaacat atcgttattc gtccgctggc ctactgccgc gagaaagata ttcagcgatt
     1681 tgccgatgca aaagcgttcc cgattattcc gtgcaacctg tgcggttcac agcctaacct
     1741 gcaacgtcag gtgattgctg acatgttgcg tgactgggat aaacgttatc cagggcgtat
     1801 cgagacgatg ttcagcgcga tgcagaatgt ggtgccgtcg catctgtgcg ataccaacct
     1861 gttcgatttc aaaggcatca cccacggttc tgaagtggtt aacgggggtg atctggcgtt
     1921 tgatcgcgaa gagatcccac tacaaccggc gggctggcag ccagaagaag atgaaaatca
     1981 gttggatgag ttacggctga atgtggttga agtgaaataa ccaggatagc gcccgatgcg
     2041 caagcgtatc gggctactct tatggaggcc ggataagacg cggccagcgt cgcatccggc
     2101 aatcccgaat aagatgttta ctcttgcacc cggcaattca acatttcatt attttaataa
     2161 ccgcacccgg cacgtttttc ctttaatctt cccgccctgt aactgtttcc atgctttatg
     2221 agcaacagcc tgacggaccg cgacatagac atgcgccgga tgcacggcga ttttgccaat
     2281 atctgcgcca tcaagcccga tatctcctgt cagtgcacct aatacatcac ccgggcgcat
     2341 tttggctttt ttcccgccat cgatacacaa cgttgccatt tctgcttcca gcgtcgcaat
     2401 ggaactatta gctggcggcg tttgccagtt aagttttatc tgcaacatgt cagaaatgat
     2461 attggcccgc tgtgcttctt ccggagcaca gaaactgatc gccagaccgc tatttcctgc
     2521 acgagctgta cgaccgatgc gatgtacatg aacttcaggg tcccacgcca gctcaaagtt
     2581 caccaccagc tcaagcgatt taatatccag accacgcgca gcaacatcag tcgcgaccag
     2641 tacacgggcg ctaccgttag caaaacgtac cagggtctga tcgcgatcgc gttgctccaa
     2701 atcgccgtgt aatgacaatg cactttgccc tacttcattc agcgcgtcgc agacagcctg
     2761 gcaatctttt ttggtattgc aaaacaccac gcaagaggat ggctgatgca agcttaataa
     2821 ccgttgcaac agaggaattt tgcctttgct ggatgtctca taaaattgtt gttcaatggg
     2881 tggcaaagca tctgttgagt caatttcaat cgccaaagga tcgcgttgca ctcgtccgct
     2941 gattgcagcg atggcttccg gccaggttgc cgaaaacaga agcgtctgtc gagatgcagg
     3001 cgcaaaacgg atgacatcat caatggcatc gctaaatccc atatccagca tgcggtcggc
     3061 ctcatccatc accagcgtat tcaacgcatc cagtgatacc gtgccttttt gcaggtgatc
     3121 cagcaaacgc cccggcgttg ccacgataat atgcggcgca tgttgcaacg aatcacgctg
     3181 cataccgaac ggttgaccac cgcacaacgt caaaattttg gtatttggca gaaaacgcgc
     3241 cagccgacgc aattcacctg ccacctgatc cgccagttca cgcgtaggac acagcactaa
     3301 agcctgggtt tgaaatagcg acgcatcaat ttgctgtaac aagccgaggc caaaagccgt
     3361 cgttttgccg ctgccggttt tcgcctgcac gcgaacatct tttccggcaa ggatcgccgg
     3421 aagcgcggcg gcctgcaccg gcgtcatggt taaataaccc aactcattaa ggttcgtgag
     3481 ttgggcggga ggcaaaacat tcagggtaga aaaagcggtc acaatctatt ctcgtggtca
     3541 tcgacgcaaa gttagcaggc gcgtatcctc gcagatctac gctcacgatg cgacaattta
     3601 atcggttctt catcgggtgg tgggtcaggc atgggttgcg ggcgagggat cggatcgggc
     3661 actggaacag gatcgccagg aatcggttca gggacaggaa tttgcaaata aataagtgtc
     3721 gtcatatttc cctctggtca ttgggtggac tcttaaaggg tagacgctga taaataacag
     3781 gcaaaaaaag ccgactcatc aaagtcggcg tcgtacgaat caattgtgct atgcagtaat
     3841 tcaaaaaagg aagtaagaca atatggagcg caacgcccat cgcttgacgt tgcattcacc
     3901 tgcaagagag atattgccct gaatgggtag agagtttatt gacttcgctc aaactttgcg
     3961 gcgtttttgt atacagacag ccggaaaaat tgcttttgtt acaaccattt actacgatgc
     4021 aaccataaag caacaccacc aataagaaca actaacagaa tacaaaaaat tgaaaatccg
     4081 aattgccacc cgccgccagg gatcccacca aggttgacgc caaataaccc ggtcagaaag
     4141 gtactgggta aaaagaccat tgccatcaac gacattgtat aggtacgacg agctaaattt
     4201 tcctgcatca cctgagcgat ttcatccgcc atcacgccag tccgtgctat acaggcgtcg
     4261 atttcgtcaa ggccgcgccc aaggcgatcg gcaatatcct gcatccgacg gcgttggtca
     4321 tcgctcatcc acggcaaacg ttcactggca agacgagcat aaacatcacg ttgcggtgcc
     4381 atatagcgac gcatcacaat taattgtttg cgcagcagag ccaggaatcc acgcggtgga
     4441 atttgctgat caaggagatt atcttcaagg tcgataattt tatcgtgcag ctgctcgata
     4501 aattcactgg aatgatcggt caacgcatcg cacacatcca ccagccatcc cccgcaatcg
     4561 gtcggacccg tgccctcttc cagatcgctc accacatcgt ccagcgccag cactttgcgt
     4621 tgtcgggtcg aaacaattaa ccgcccgtcc atatatacac gcatggcgac cagttgatcg
     4681 gggcgttcat cggtgctgcc gtttatacag cgcaatgtaa tcagcgtgcc ttcaccgaga
     4741 cggctgactc ggggacgcgt gctctcgccc gccagcgcat cacgtacgtt attgggaagc
     4801 agcggtgttg tcgccagcca ttgggcgcta tcatggtgta cataattaag gtggagccaa
     4861 cagggatgcg cttcatcaat cacatctgta ttttccagcg gtttaacgcc gcctctacca
     4921 tccagcatcc aggcaaatac tgcatccggg acattaacgt ccgatccctt aatcgcttcc
     4981 acagtgcctc catcatcaac gcattatttt gtagtctagc cttctggccc tgttacgcaa
     5041 catctcatca ccccattacc ctgaaatgat taataaaatt ctgtctaaat tgaatacaaa
     5101 aagcaaaatg cttttccgta tacaaaccgt gtgaagtgtt aaatagcgtc tatcattatc
     5161 agaattatct gatcatatga cgtggctttt ttgcgatcgg atagcaacaa aaattgataa
     5221 aaataacggg atctcaatga ttacgcacaa cttcaatacc ctggacttac tcaccagtcc
     5281 tgtctggatc gtttcgccct ttgaggaaca gttaatttat gccaatagcg cggcgaaact
     5341 gttgatgcaa gacctcacgt ttagtcagct acgaaccgga ccctattccg tctcctcaca
     5401 aaaagaactg ccgaaatacc tctccgatct gcaaaaccaa cacgatatta tcgaaatcct
     5461 cactgttcag cgtaaagaag aggaaacagc attgagctgt cggcttgttt tgcgaaagct
     5521 gacagaaaca gaaccggtga ttattttcga aggtatcgaa gcgccggcaa cgctgggttt
     5581 aaaagccagt cgctcggcaa attatcagcg caaaaaacaa ggtttttatg cgcgcttttt
     5641 tctgactaac tctgcaccaa tgttgttgat tgacccgtca cgagatggac aaatcgtcga
     5701 tgctaacctc gccgcgctca atttctatgg ttataaccat gaaacgatgt gccagaaaca
     5761 tacctgggaa ataaatatgc tcgggcgtcg cgtcatgcct atcatgcatg aaatctcgca
     5821 tttacccggt ggtcataaac ctttgaattt tgttcataaa ctggcggatg gttcgactcg
     5881 tcatgtgcag acctatgccg gaccgattga aatttatggc gacaagctca tgttatgtat
     5941 tgtgcatgat attactgagc aaaaacggct ggaggagcag ctggaacatg ctgctcacca
     6001 tgacgcgatg accggattac tgaatcggcg acagttttat cacattacgg aaccaggcca
     6061 aatgcagcat ctcgccatcg ctcaggatta cagcttgttg ctcatcgaca ccgatcgttt
     6121 taaacacatt aacgatctct atgggcattc taaaggtgat gaggtgttat gcgccctcgc
     6181 ccgcaccctc gaaagttgcg ctcgcaaagg cgatttggtg tttcgttggg gaggcgaaga
     6241 gtttgtctta ttgctaccaa gaaccccact ggataccgcg ctttcgctgg ctgaaactat
     6301 ccgcgtaagc gtggcaaaag tgagtatttc gggcttacca cgctttaccg tcagcattgg
     6361 tgtggcgcat cacgaaggaa atgaaagcat cgatgaactg tttaaacgcg ttgatgatgc
     6421 tttgtatcgg gcgaaaaatg atggacgcaa ccgcgtgctg gcggcataag ccgcggatgc
     6481 gtctcgagat caacgactgc gcttagcgtg gcgctcccag ttttcttgct tcgcctgcgc
     6541 cgttttacgt agtgcgacgt aacacgcccc gctgccgcca tgatgcggta gcgcggtgca
     6601 atatgcctga acatcatcaa attcggtcag ccagcgcgcc acatagctgc ggacaatatt
     6661 ggcatgcgat ttatcatccc gccctttacc atgaataatc agcacgttac gcaaaccatc
     6721 cgccagggct tgttgaataa aactgaacac cattttgcgg cactcttcca ccggctggcg
     6781 caaaagattc aggctcgcct gttgcggata tttaccactg cgcagcttat ccagcacccc
     6841 atgttgcaac ccttcccgcc gaaactccag cggctgactt agtgggatga tgtcgagaaa
     6901 tccggtggtg aggaaattat caagctgcag cgtgtcgata cgctgcggcg cacgttggtt
     6961 acgcgttgga tgccagtgga catcggtagc acgtttcagc ggctggacat cttccatggc
     7021 gtcaagaaac agcgatttgt cgtcaaggtt catgttacat cctcccgcaa ttaagagcgc
     7081 gatatgataa ccagaccggg tcggtccaac aacgtattac ccaaatttcc agtaataagt
     7141 tccaaatatt gccgatattt taagcaaaat actt
//
LOCUS       DAPHBJ010000214         7171 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.216, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000214 DAPHBJ010000000
VERSION     DAPHBJ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.216"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(229..1365)
                     /locus_tag="RHZ76_003954"
     CDS             complement(229..1365)
                     /locus_tag="RHZ76_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV2814501.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1368..1730)
                     /locus_tag="RHZ76_003955"
     CDS             complement(1368..1730)
                     /locus_tag="RHZ76_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDV2814502.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2267..4180
                     /gene="mtlA"
                     /locus_tag="RHZ76_003956"
     CDS             2267..4180
                     /gene="mtlA"
                     /locus_tag="RHZ76_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDV2814503.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4410..5558
                     /gene="mtlD"
                     /locus_tag="RHZ76_003957"
     CDS             4410..5558
                     /gene="mtlD"
                     /locus_tag="RHZ76_003957"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDV2814504.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5558..6145
                     /gene="mtlR"
                     /locus_tag="RHZ76_003958"
     CDS             5558..6145
                     /gene="mtlR"
                     /locus_tag="RHZ76_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HDV2814505.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6157..6366)
                     /locus_tag="RHZ76_003959"
     CDS             complement(6157..6366)
                     /locus_tag="RHZ76_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814506.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6651..7013
                     /locus_tag="RHZ76_003960"
     CDS             6651..7013
                     /locus_tag="RHZ76_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HDV2814507.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTAF"
BASE COUNT         1814 a         1811 c         1834 g         1712 t
ORIGIN      
        1 attagagccg tcgcaggagg aacaactcat atcatcattg cgatctcgac gatgtatagt
       61 ctcatcctgc ggcagaacaa gacggataaa aaatccaatg agagcacact catccagaac
      121 ttattaacat aatgaatatt aaagaaaaat catcgcagct aatgaagtaa tatgcaaaaa
      181 taattagtcc ttacaaggag caaagttcca taatctcccc cctccccctc aatgatccaa
      241 ataaagataa tgcatccagc tggtcattct tagcagcact ttccgcatca ccgaaacatg
      301 gctgaaatgg tcggagtaaa ccgccacctg ggcgtagatg ccgtcgggta aggcatcgat
      361 atcatcgtta gggtccagtt caatggttcc cagcacaccg tccgtgccgg gcacgaccgt
      421 taatgattgc aataccccct gcgcctgata agaaccgcct ggcacgacag gtaaaatact
      481 agtcagtttg ccgtgaaaca cctgcccagg tagcgcgtta aacaccactt ccgcatcatc
      541 gccgggtttc agacgtaaca gcgagttttg ccgaaattgg gcgacaattt gccgtttttg
      601 ctcggggatg aagaccatca ccggacgcag cggcaaggca gctgcgtatg tacctgggcg
      661 gatcagtacc tgagtaacgt agccattgct cggcgcgcga atgacagtct gctcaaggtt
      721 atattttgct tcagtaagtt gcgctcttaa gctcacaatc tgcgattgct cgccgttaac
      781 catactgtcg agctggctct ggatctgcgc ctgctccgcc accgagcctt tcaccagcgc
      841 atcctgcgcg aggaaatttt gccgcgcatc gtcgatgtca cgttccgaga acggattcac
      901 cgccgcctgg ctgcctttca agtaacgttg ataattttta aacagacggt cgcgctccgc
      961 tgaaacctgg gtggtgttgg cctgcgcttc agtgagctgc gcacgcagcg tctttatatt
     1021 atgcgtcgcc gtcatcaggt cagcctgaag tctgtcaact cgcgcctggt aacgaaccgg
     1081 gtcgagctta aaaagcacct cgcccttttg aataagctga ttattcttgt cagtgacttc
     1141 agtaacaatt cccgtcacct gtggcgtgat agggatcgct atcactgcct tttgcgcggt
     1201 aaaagtgtaa gggtggttgt agttcatcaa caaaatcaaa ccactcacca gaaacacgcc
     1261 tcccagcgcc gccgtcgcca gcgtccactg atttaccgga atgcggaaga ttttaaagac
     1321 cgcccacgcc agcgccacgt aagttaaaac aatcaataga tccataatta gatctccggg
     1381 aacgtggaat agtcagtctt tttctcggcg gcgagttgat gctccagccg ggaaattcgg
     1441 tcagaaagtg aggcgatttc cgggtcagtc gctttctcct gcgacgcaac atgtgactgc
     1501 ataccccagc cacgctccgg ttgatagagc gtcgcccaga tccacagaaa cggccagata
     1561 acatgcagag taaacaggct cacccagccc gccgtatgaa tagcgtcggc atgggggtga
     1621 ttgcgctttt tggcaatcag ataagggatg tcatgtatcg cgatgattcc ataaaaaatc
     1681 accagaaaga cgaagatcag cactcccaac gcgaaatagt ttagaaacat atctgcctcg
     1741 gattcacgtt tatcagtgtt gtttttgggc tggcagccag aagggagtca ggctgatatt
     1801 ttgacaataa tccgggttcg cgattctcgc cataacacca aagaataatt tttagaggtg
     1861 atgagttgct aagttacata acgattgtat gacgaaggca taacatgctg tagatcacat
     1921 caggtgaacg ccgtaagaaa atatcttgtg attcagatca caaagattca acaaaccatc
     1981 aaaacaaaaa tgtgacacta ctcacattta aatgccattt ttagcgaaaa tcgccgcctt
     2041 gttgcttttt tacacaagcg ttttgtgatg aacgtcacgt caattacctc tctaccccct
     2101 atatttatgt gattgatatc acacaaaagg ccgtcgactg gacagttaac cgattcagtg
     2161 ccagatttcg cagtatctac aaggtccggc tacctctgcc gccacattaa caaaaaacct
     2221 cgggcttcca gcctgcgcga cagcaaacat aagaaggggt gtttttatgt catccgatat
     2281 taagatcaaa gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc caaatatcgg
     2341 cgcgtttatc gcgtggggta tcatcaccgc gttatttatt ccaacagggt ggttaccgaa
     2401 cgagacgctg gcgaagctgg tcgggccgat gatcacttat ctcctgccgc tgctgatcgg
     2461 ttataccggt ggtaagctgg taggcggcga acgtggcggc gtagtcggtg ccatcaccac
     2521 catgggcgtt atcgtcggcg cagacatgcc gatgttcctc ggttctatga ttgcaggtcc
     2581 gctgggcggc tggtgcatta agcacttcga ccgctgggta gacggtaaga tcaaatccgg
     2641 ttttgagatg ctggtgaata acttctccgc aggcatcatc gggatgatcc tcgctattct
     2701 ggcattcctc ggcattggcc cgattgttga agccctgtcc aaaatgctgg ctgcgggcgt
     2761 taacttcatg gttgtccatg acatgctgcc gctggcgtct atctttgttg aaccggcgaa
     2821 aatcctgttc ctcaacaacg ccattaacca cggtatcttc tcgccgctgg gtattcagca
     2881 gtcccatgaa ctgggtaaat caatcttctt cctgattgaa gctaacccag gtccaggtat
     2941 gggcgtgctg ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg
     3001 tgcggcaatc atccacttcc tggggggtat ccacgaaatc tacttcccgt atgtgctgat
     3061 gaatccgcgt ctgatcctcg cagtcatcct cggcggtatg actggcgtgt tcacgctgac
     3121 tatactgggc ggtggtctgg tttctccggc atctccgggt tctatccttg ctgtactggc
     3181 gatgacacca aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg cgatggctgt
     3241 ctccttcgtt gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag aagatgatat
     3301 tgaagcagca actcgtcgta tgcaggacat gaaagctgag tctaaaggca catctccgct
     3361 gtctgcgggc gatgtgacta acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga
     3421 cgccggtatg ggttccagtg cgatgggcgc aggcgtgctg cgtaagaaaa ttcaggatgc
     3481 aggtctgtca cagatttcgg tcactaacag cgcgatcaac aacctgccgc cagatgtgga
     3541 cctcgtcatc actcaccgtg acctgaccga acgcgctatg cgccaggttc cgcaggcgca
     3601 gcatatttcg ctgaccaact tcctcgacag cggcctgtac accagcctaa ccgaacgtct
     3661 ggttgctgcc cagcgccata ctgaaaacga agtgaaagtg aaagacagtc tgaaagacag
     3721 ctttgacgat tccagtgcta acctgttcaa actgggcgcg gagaacatct tcctcggtcg
     3781 caaagcagca accaaagaag aagcgattcg ttttgctggc gagcagctgg tgaaaggcgg
     3841 ttacgttgag ccggaatacg ttcaggcgat gctggatcgt gaaaaactga ccccgactta
     3901 tctgggtgag tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc gcgtactgaa
     3961 aacgggcgtg gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag aagaagatga
     4021 cattgcccgt ctggtgattg gtattgcagc ccgtaacaac gagcacattc aggttatcac
     4081 cagcctgacc aatgcactgg atgatgagtc cgtcatcgag cgtctggcac acaccaccag
     4141 cgtggatgaa gtgctggaac tgttggcagg tcgtaagtaa tccaatccca ccctctccac
     4201 atggagaagg tggggttaat tgcctgatgc gctacgctta tcaggcctac aggatgcatc
     4261 acgatttgtt gaatttgcac gttcttgtag gccggataag gcgcttacgc cgcatccggc
     4321 gctgcccctc tcctcacgga gagggtttgg gtgagggaaa agcctcaccc cagccctctc
     4381 gggtaaaaac attgatgaag gttaatacta tgaaagcatt acattttggc gcaggtaata
     4441 tcggtcgtgg ctttatcggt aaactgctgg cagacgcggg tatccaactg acgtttgccg
     4501 atgtcaatca ggtggtactt gatgccctga atgcccgtca tagctatcag gttcatgtgg
     4561 ttggtgaaac cgagcaggta gataccgttt ccggcgtcga tgctgtcagc agcattggtg
     4621 atgatgtcgt tgatctgatt gctcaggtcg atttagtcac taccgccgtt ggcccggttg
     4681 tgctggaacg tattgctccg gctatcgcca aagggctggt gaaacgtaaa gaacaaggta
     4741 atgaatcccc gctgaacatc atcgcctgtg aaaacatggt acgcggtacc acgcagctga
     4801 aaggtcatgt gatgaacgcc ctaccagaag acgccaaagc gtgggtagaa gaacacgttg
     4861 gctttgtcga ttccgccgtt gaccgcatcg taccgccttc ggcttcggca actaacgatc
     4921 cgctggaagt gacggtagaa actttcagcg aatggattgt cgataaaacg cagttcaaag
     4981 gcgcactgcc gaacatccca ggcatggagt taaccgacaa cctgatggca tttgtcgaac
     5041 gtaaactctt caccctgaac acgggtcatg ctataaccgc gtacctcgga aaactggccg
     5101 gtcatcagac cattcgtgac gctattctcg acgagaaaat ccgcgcggtg gtaaaaggtg
     5161 cgatggaaga aagtggtgcg gtactgatca agcgctacgg ctttgacgca gacaaacatg
     5221 cggcgtacat ccagaaaatc ctcggtcgtt ttgagaaccc gtatctgaaa gatgatgtag
     5281 agcgcgtagg ccgtcagccg ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccac
     5341 tgctcggtac gctggaatac agccttccgc acaagaatct gattcagggg attgctggtg
     5401 caatgcactt ccgcagtgaa gatgatccgc aggctcagga actggcagca ctgatcgctg
     5461 acaaaggtcc gcaggcggcg ctggcacaga tttccggtct tgatgccaac agcgaggttg
     5521 tatccgaggc ggtaaccgct tataaagcaa tgcaataatg gtggaccagg cgcaggacac
     5581 cctgcgcccg aataacagat tgtcagatat gcaggcaaca atggaacaaa cccaggcctt
     5641 tgaaaaccgt gtgcttgagc gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac
     5701 cgccgtcgag cttttgaccg aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga
     5761 cgattacgcg gtgaagtatg ctgtagaacc gttactcgac ggcgacggtc cgctgggcga
     5821 tctttctgtg cgtttaaaac tcatttacgg gttgggcgtc attaaccgcc aggaatacga
     5881 agatgcggag ctgctgatgg cattgcgtga agaactaaat cacgacggca acgagtacgc
     5941 ctttaccgac gacgagatcc ttggaccctt tggtgaactg cactgcgtgg cggcgttacc
     6001 accgccgcca cagtttgaac cagcagactc cagtttgtat gcaatgcaaa ttcagcgcta
     6061 tcaacaggct gtgcgatcaa caatggtcct ttcactgact gagctgattt ccagaatcag
     6121 cttaaaaaaa gcctttcaaa agtaagcaac gtctgcttac tgcccctcta cctgcttcgg
     6181 ccgataaagc cgacgataat actccagacg ttgtagatat aacggcacgc tttcatcggg
     6241 tattccggac ggaatcgcgt tacggggagg aagttttttc agatactccc ggaacgcctg
     6301 gcttgatgcc atgaaatcta cggctttatc gataagaagc ggaacgtttt cacctaattt
     6361 gcccatgatg atatctccgt attaccccgc gccgggaatg cgcggccgcc aattttagtt
     6421 taggttcccg gtaaagatga cattaaggaa aacgtgctga atcctcaata atcatccgct
     6481 gctgtttatt atcttttttc tatagatttc ttaattaatc aacgaattgc gtagtttttg
     6541 agcatgttaa tgattacgtt gcagaactat tacaatgtca tcagttgtga acaaagcacc
     6601 tggtcgcgca tactaggggc tataaattta tctttatcag aagccatcac atgaaagaag
     6661 tcgaaaaaaa cgaaatcaaa cgtctcagcg atcgcctgga cgccatccgc caccagcagg
     6721 ccgatctgtc gctggttgaa gccgcagaca aatatgccga gctggaaaaa gagaaagcca
     6781 cgctggaagc agaaattgct cgcctgcgtg aggttcatag ccaaaaactg agtaaagaag
     6841 cacagaagct gatgaagatg ccgtttcagc gcgcaattac caaaaaagag caggctgata
     6901 tgggcaagct gaagaaaagt gttcgcggac tggtggttgt gcacccaatg accgcactgg
     6961 gccgtgaaat gggcctggaa gagatgaccg ggttttcaaa gaccgcgttt taagcccctc
     7021 agacgactgg tacgatcgta tcaacccgca tctgacttgc gttagctggt caatgcgggg
     7081 actctcctgt ctactcgcct aatgaccaaa caattcactc cctttacttc gatttaccgc
     7141 tggttgatat tctgaaaatg catctttacc g
//
LOCUS       DAPHBJ010000215         7094 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.217, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000215 DAPHBJ010000000
VERSION     DAPHBJ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.217"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            160..1494
                     /gene="adeQ"
                     /locus_tag="RHZ76_003961"
                     /gene_synonym="yicO"
     CDS             160..1494
                     /gene="adeQ"
                     /locus_tag="RHZ76_003961"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HDV2814508.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAIVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1547..1999
                     /locus_tag="RHZ76_003962"
     CDS             1547..1999
                     /locus_tag="RHZ76_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HDV2814509.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2210..3400
                     /gene="nepI"
                     /locus_tag="RHZ76_003963"
     CDS             2210..3400
                     /gene="nepI"
                     /locus_tag="RHZ76_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HDV2814510.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3441..3734)
                     /locus_tag="RHZ76_003964"
     CDS             complement(3441..3734)
                     /locus_tag="RHZ76_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814511.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3956..4774
                     /gene="nlpA"
                     /locus_tag="RHZ76_003965"
     CDS             3956..4774
                     /gene="nlpA"
                     /locus_tag="RHZ76_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HDV2814512.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            complement(4778..5701)
                     /locus_tag="RHZ76_003966"
     CDS             complement(4778..5701)
                     /locus_tag="RHZ76_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV2814513.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLSVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(5812..6996)
                     /locus_tag="RHZ76_003967"
     CDS             complement(5812..6996)
                     /locus_tag="RHZ76_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HDV2814514.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTTAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
BASE COUNT         1779 a         1598 c         1785 g         1932 t
ORIGIN      
        1 attttatcaa tttgattatt atttatcata aagaaacgtc gcgagattat taaagaatat
       61 ccattaatgt gcaattgaaa tgtgataatt atcactgaac cgttgttgtc atttgtttta
      121 caaaagcaag ggattaaatc tcctccaatg gacaaaaaaa tgaataatga caataccgat
      181 tacgtgagta atgaatcagg gacgctttcg cgattattta aactacctca gcatgggacc
      241 accgtccgca cagaattgat tgcggggatg accacttttt taaccatggt gtacatcgtt
      301 tttgtgaacc cgcaaatcct cggcgcggca caaatggacc cgaaagtggt gtttgttacc
      361 acctgtttga ttgccggtat cggcagtatt gcgatgggga tatttgctaa cttacccgtg
      421 gcgctggctc cggcaatggg gctgaacgcc ttctttgcct tcgtggtcgt gggggcgatg
      481 ggcatctcct ggcagaccgg gatgggcgca atattctggg gcgcagttgg actatttttg
      541 ctcacgctgt ttcgtatccg gtactggatg atctccaaca ttcccttaag tttacgtatt
      601 ggtatcacca gcggaattgg attatttatc gccttaatgg gattaaaaaa tactggcgtt
      661 attgtcgcca ataaagacac gctggtgatg attggcgatt taagttctca cggcgtgttg
      721 ttaggtattt tagggttttt tattataacc gtgttgtcat cacgtcattt tcatgccgcg
      781 gtgctggttt ctattgtggt gacgtcttgc tgtggattat ttttcggtga tgttcatttt
      841 agcggcgtct attccattcc gcctgatatt agcggcgtca ttggtgaagt agatttgagc
      901 ggcgcgttaa cacttgaact cgccggtatc attttctcct ttatgctgat caacctattt
      961 gattcatcag gaacattaat tggtgtaact gataaagcgg gcttaataga tggtaacggt
     1021 aaattcccca atatgaataa ggcgctgtat gttgatagcg tcagttcggt ggcgggtgcg
     1081 tttatcggca cctcgtctgt tactgcctat attgaaagta cttctggtgt ggcagtcggt
     1141 ggccgcacgg ggctgactgc ggttgtggtt ggcgttatgt tcctgttggt tatgttcttc
     1201 tcaccgctgg tggcgatagt tcctccttac gcaaccgccg gagcgttaat ctttgttggc
     1261 gtgctgatga cttcgagcct ggcgcgcgtt aactgggatg attttaccga atcggtgcct
     1321 gcgtttatta ccacggtgat gatgcccttt actttctcga tcaccgaagg gattgcactc
     1381 ggctttatgt cgtactgcat catgaaagta tgcaccgggc gctggcgcga tctgaacctg
     1441 tgtgtggtgg tggtcgcagc tctgtttgca ctgaagatta ttctggtgga ttagtaactg
     1501 gcgagggttt ccagatatca tgagttctga ttacgcagga gaactcatga tctggataat
     1561 gctcgccacg ctggcggtag tgtttgtggt tggttttcgg gtgctgacat ccggggccag
     1621 aaaagcgatt cgccgtctca gcgatcggct gaacatcgat gtcgtacccg tggagtcgat
     1681 ggtcgatcaa atgggaaagt cagctggtga cgaattttta cgttatttgc atcgtccgga
     1741 tgagtcgcac ctgcaaaacg ccgcgcaggt gttgctcatc tggcaaattg tcattgtcga
     1801 tggtagcgaa cagaacctgc tgcaatggca tcggatttta caaaaagctc gcctcgccgc
     1861 gccgattacc gacgctcagg tcaggctggc gctaggtttt ctgcgcgaaa ccgaacctga
     1921 aatgcaggat attaatgctt ttcagatgcg ctataacgcg ttctttcagc ctgccgaggg
     1981 cgttcactgg ttgcattgaa accgtttcgt cttatcagcc tacagaccgc gcctgacctg
     2041 tcgggtggat aagacgttca cgccgcatcc ggtaatcttt gccctgttcc gtttcgatct
     2101 taactccagc tcaagcaaca ccgtaaaagt tgcaaaaacg ttaaatgcgt cacacatttc
     2161 aatgttaaag tggtcggctt ttcccctgaa acatgccacg ggtaacacca tgagtgaatt
     2221 tattgccgaa aaccgcggcg cggatgccat cacccgaccg aactggtcag ccgttttctc
     2281 ggtggcgttt tgtgtcgcct gtctgattat cgttgagttt ttgcccgtca gtttgttgac
     2341 gccaatggcc caggatttag gcatttcgga aggggttgcc gggcaatcgg tgaccgtgac
     2401 cgcctttgtg gcaatgtttg ccagtttgtt tattacccag acaattcagg ctactgaccg
     2461 ccgctacgtt gttattttgt ttgccgtttt gctgacgctc tcctgcttgc tggtttcctt
     2521 tgctaactca ttcagtttgc ttttaatcgg tcgtgcctgt ctggggctgg cgctgggcgg
     2581 gttctgggcg atgtcggcgt cgctgaccat gcgtctggtg ccgccgcgta cggtgccgaa
     2641 ggcgctgtcg gtgatcttcg gcgcggtttc tattgcgctg gtgattgccg cgccgttggg
     2701 cagtttttta ggcgagctta tcggttggcg caatgtcttt aatgcggcgg cggtgatggg
     2761 cgtgctgtgt attttctgga ttatcaaatc attgccttca ctgccaggcg aaccctcgca
     2821 tcagaaacaa aatactttcc gcttattaca acgtccgggt gtgatggcag ggatgatcgc
     2881 catcttcatg tctttcgccg ggcagtttgc tttcttcacg tatattcgcc cggtgtatat
     2941 gaacctggcg ggattcggcg tggatggctt aacgctggtg ctgttgagtt ttggtatcgc
     3001 cagctttatt ggtacgtcgc tttcgtcgtt cattcttaaa cgttcggtaa aactggcctt
     3061 agcaggcgcg ccgttaatac tggctgtgag tgcgttggta ctgacgttgt ggggaagcga
     3121 taaaatcgtt gctaccggcg tggcgattat ctgggggcta acttttgcat tggttcccgt
     3181 cggctggtca acgtggatca cccgctcgct ggccgatcag gcagaaaaag ccgggtctat
     3241 tcaggtggcg gttattcagc ttgctaatac ctgtggcgcg gcaatcggcg gttatgcgct
     3301 ggataatatt ggtctgactt cgccgttgat gttgtccggc acattgatgt tgctgactgc
     3361 attgttggtt actgcaaagg tgaaaatgaa gaaatcctga tatcaggtaa agaagcgttg
     3421 gtcagtagtt gttgctgttc ttacatatcc gggcattcta ccttacccaa cgcttcccaa
     3481 taattgctct gattattacg ttccatcgcc acgatggcct cgcgtatatg ttgtttattg
     3541 ttttcatctg acaacattaa cttttcccac gcttcatctg ataatgacgc ctgtggcgtg
     3601 caaattttac gtaaatcttg taataccgtg gttttctctt ttgcggactg cagtgagcta
     3661 aaaggcgact cggcgtatac gatgcctggc atagcgaaaa aagtgaaaat aagcagtagt
     3721 gtcgttggtt tcatggattc aaccttttac aaatgtgtat cgaatgaagc ataagtgtag
     3781 tttgctttca tcgctctcat catgctgtac gaaacgcgct tgcgtggtca gtaagaagtg
     3841 ccagacttta tattccactt ttattccttt ttattcttat cgatagcgtt tcgtttttta
     3901 aaccgcagcg accttaccgc tatagtcagg taatcattaa taaaaggata aaaaaatgaa
     3961 actgacaaca catcatctac ggacaggggc cgcattattg ctggccggaa ttctgctggc
     4021 aggttgcgac cagagtagca gcgatgcaaa acacattaaa gttggcgtta taaatggcgc
     4081 agaacaagat gtcgcggaag tcgctaaaaa ggtggcaaaa gagaaatatg gcctcgatgt
     4141 tgaactggta ggatttagcg gttcgttgct accgaacgat gcgacaaatc atggcgaact
     4201 tgatgcgaat gtcttccagc atcgaccctt tcttgaacaa gataatcagg cgcatggtta
     4261 taaactggta gcggtgggaa atacgtttgt cttccctatg gcgggttatt ccaaaaaaat
     4321 caaaaccgtg gcgcaaataa aagaaggcgc gacagtggcg atccccaacg acccgaccaa
     4381 ccttggtcgc gcacttttac tgttgcaaaa agagaagcta attactctga aagagggaaa
     4441 agggttatta cctaccgcgc tggatataac cgataatcct cgtcatttgc agattatgga
     4501 actcgagggg gcgcagctcc cgcgcgtact ggatgatccc aaagttgatg tggcgattat
     4561 cagcaccact tacattcagc agaccgggct ttctccggtg cacgacagcg tatttattga
     4621 agataaaaat tcgccgtatg tgaatatttt ggtggcacgg gaagataata agaatgcaga
     4681 aaacgtgaag gaatttctgc aatcttatca atcatccgaa gtcgctaaag cggcagaaac
     4741 catctttaac ggtggcgcgg tgcctggctg gtaattctca ctcatcccgc gccggacgat
     4801 ttatttttct ggcgcggcga cgggaatcca ttgaaatcaa aattaccgat gacagaatca
     4861 gcagcgttcc cagccagtcc ggtaatgtga acgtgatccc cagcaacagc aaagagagta
     4921 gcgcgctact taatggttct gcacagctca aaatgctggc ttttggaccg ccaattaatt
     4981 gtgctccttt caggtacaga ctaaatgtca gggacgtacc aatgacgatc aaataaaaaa
     5041 acgccaaaat caaactgccg ttaacgacaa agtttgtccc ttgtctggca taaaaaggca
     5101 acagaatcag accgccgatc agcatactcc agccgacgac agacaacgtg ccatagcggg
     5161 caattagcgt tgagggatag gtggtataga atgcagcagc aaatgccgag gcaatgcccc
     5221 agaacaacgc ggcaggagag atcgataatg acgtcggatt accgtgtgtc accaataaaa
     5281 aagtcccgac cagcgatgtc aaaatagcgc agaaaaccag aatgcccggg cgcgatttac
     5341 gcaccagtga gaaccaggcg acgataatcg tcggtgagag gaattgcagc accgttgccg
     5401 tggctgcgtt cgatttttcg atggttagca aaaaagtgag ctgtacagtt agcgcgccaa
     5461 ccacggaaaa aatcagcagg ctaatggcat ctttatgatt gttaataata gaaaagattt
     5521 tatcgccatg aacaaatgac agcgtcagta gaatcaaacc ggcgaatatc aaacgcgtca
     5581 tagtcaaaaa ctgcgacgac atctggcttt gctccatgat gtattgcgcg cagacccctg
     5641 aacttcccca caacacggcg gcaatcagaa cgttcagcat cccctttctg gtggaaccca
     5701 tttttccctc tgcatactgt tttgtcgttt atgtatgcag gcatgatagc aaaaatggcg
     5761 aggatggctc tgtttgcgcc acgccgattt ttcttagccc agactggtga gctaaatatc
     5821 tttaataaac agcaggcaaa tcatcgcaat acccaacatc cctatcgcca gccagaacaa
     5881 cgcatggtag ctccagattt caaccataat tccgtcaaca gagccggcga taatccagcc
     5941 gacgcgtgaa gtatttgcat ataacgttgt cgcagagcct attttttcag gcatcaggtc
     6001 ctgaaaataa agcatgccga taccacagag aataccaagg aagatggcat taagaatttg
     6061 caattccagc tcaaccgccg gagtcgtcgc catgagtacg ctggcgtaaa aacacattcc
     6121 actcacgata gcaatgagca ttaatagtcg cttgccaata cgtttcatgt aatagcctgc
     6181 gattaacatc atcggaattt ccagaccggc agcgatacca atcatttctc cagccagttt
     6241 atcggttaga tgcagttcat caataataaa tagcggcata tttatcatgt agagattatt
     6301 cgccgcccac atcattgaac agaccacaaa aagtagccgc gtatcccgct tcctgtgggt
     6361 ggagggtaaa atttctacgg gttgggtaac gacaggaata tttctttgta ttgatggcaa
     6421 aaacaaccag actatcagcc cgcaaacaac aaatgcgatg gcagtggtga gatacatcac
     6481 tttaaaacta aatcccattg ccagttcata agcgagcggt ggcccgataa cccaggcaag
     6541 cgagatctga gcacgtaaaa atgtactgaa catgaccgtc tcacggcctg ttctgtcggc
     6601 gtgttcacgg gcgagggcga acatttgcgg gtttgcggtg gaagcaaaac tactcagaag
     6661 tacgcccgtt gagaggagaa tgaagtagtt gcgattccac gcaaaaagcg tgcaggccag
     6721 cactccaaat aagcaacata gcagaatcag taatttacgg tcgccttgtt tatcggagtg
     6781 ccttgccaga aattgactga ccagaattcc cataatagcg ctaccggtga agaaaaaacc
     6841 taccattata ggacgggctt tcagttcatc tgcgaggaat atacttaggg taggagtctg
     6901 aagagcgccc gcaatacccg tcagaaaggc gacaagtaaa aatgccgcgg cagtgagatc
     6961 aagtattttt gatggagtgg tagccgtttt ttgcattctt ctgaatcacc gggcgaaaaa
     7021 atataactga gttacaaata agtaccagtc gaatgagtat tgtattaaga atttttgagt
     7081 atcgctgata caac
//
LOCUS       DAPHBJ010000216         7055 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.218, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000216 DAPHBJ010000000
VERSION     DAPHBJ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.218"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            46..894
                     /locus_tag="RHZ76_003968"
     CDS             46..894
                     /locus_tag="RHZ76_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2814515.1"
                     /translation="MGLSQSAVSHSVKELENHTGVRLLDRTTREVVLTDAGQQLALRL
                     ERLLDELNSTLRDTGRMGQQLSGKVRVAASQTISAHLIPQCIAESHRRYPDIQFVLHD
                     RPQQWVMESIRQGDVDFGIVIDPGPVGDLQCEAILSEPFFLLCHRDSALAVEDYVPWQ
                     ALQGAKLVLQDYASGSRPLIDAALARNGIQANIVQEIGHPATLFPMVAAGIGISILPA
                     LALPLPEGSPLVVQRITPVVERQLMLVRRKNRSLSTAAEALWDVVRDQGNALMAGREG
                     DPLYQI"
     gene            complement(885..1226)
                     /locus_tag="RHZ76_003969"
     CDS             complement(885..1226)
                     /locus_tag="RHZ76_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HDV2814516.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGGDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(1346..1421)
                     /locus_tag="RHZ76_003970"
     tRNA            complement(1346..1421)
                     /locus_tag="RHZ76_003970"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1386..1388),aa:Lys,seq:ttt)
     gene            complement(1426..1501)
                     /locus_tag="RHZ76_003971"
     tRNA            complement(1426..1501)
                     /locus_tag="RHZ76_003971"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1466..1468),aa:Val,seq:tac)
     gene            complement(1548..1623)
                     /locus_tag="RHZ76_003972"
     tRNA            complement(1548..1623)
                     /locus_tag="RHZ76_003972"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1588..1590),aa:Val,seq:tac)
     gene            complement(1668..1743)
                     /locus_tag="RHZ76_003973"
     tRNA            complement(1668..1743)
                     /locus_tag="RHZ76_003973"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1708..1710),aa:Val,seq:tac)
     gene            2002..3417
                     /gene="gltX"
                     /locus_tag="RHZ76_003974"
     CDS             2002..3417
                     /gene="gltX"
                     /locus_tag="RHZ76_003974"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDV2814517.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(3469..3861)
                     /locus_tag="RHZ76_003975"
     CDS             complement(3469..3861)
                     /locus_tag="RHZ76_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDV2814518.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(3863..4222)
                     /locus_tag="RHZ76_003976"
     CDS             complement(3863..4222)
                     /locus_tag="RHZ76_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDV2814519.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            4443..4518
                     /locus_tag="RHZ76_003977"
     tRNA            4443..4518
                     /locus_tag="RHZ76_003977"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4476..4478,aa:Ala,seq:ggc)
     gene            4558..4633
                     /locus_tag="RHZ76_003978"
     tRNA            4558..4633
                     /locus_tag="RHZ76_003978"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4591..4593,aa:Ala,seq:ggc)
     gene            4842..7031
                     /locus_tag="RHZ76_003979"
     CDS             4842..7031
                     /locus_tag="RHZ76_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HDV2814520.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALESREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
BASE COUNT         1643 a         1681 c         1842 g         1889 t
ORIGIN      
        1 gtcacagtag cgcaggagaa aagttttagt cgtgcaggag agcgtatcgg cctgagccag
       61 tcggcagtga gtcacagtgt gaaggaactg gaaaatcata ctggtgttcg cctgctggac
      121 agaaccacgc gtgaagtggt gcttacagat gcagggcagc agctggcttt gcgtcttgag
      181 cgactgctgg atgaactgaa cagcacgttg cgcgataccg ggcgtatggg gcaacaactg
      241 agcggaaaag ttcgggtcgc tgccagccag accatttccg cgcatcttat tccgcaatgc
      301 attgctgaaa gccatcgccg ctatccagat attcagtttg tcctgcacga tcgtccgcag
      361 cagtgggtaa tggaaagtat tcgccaggga gatgtcgatt ttggcatcgt catcgatcca
      421 ggcccagtgg gcgatctgca atgtgaagcg attctttccg agcctttctt tctgctttgc
      481 catcgcgata gcgctttggc cgtggaagat tatgtaccct ggcaggcatt acagggagcg
      541 aaattggtgt tgcaggatta cgcgtcaggc agccgaccgc tgattgacgc agcgctggcg
      601 cgcaatggta ttcaggcgaa tattgtgcag gagattggac atccggcgac gctgttcccg
      661 atggtagccg caggcatcgg catcagtatt cttcccgcac tggcgctacc attgcctgaa
      721 ggtagcccac tggtggtaca gcgcattacg ccggttgttg aacgccagtt gatgctggtg
      781 cgcaggaaga accgttcact ttccaccgcc gctgaagcgt tatgggacgt ggtacgagac
      841 cagggcaatg cgctaatggc tggtcgggaa ggggatccgt tgtatcagat atagatatcg
      901 atatgagtcg ttttttcaac aggatttaga agggatacat tcagagacaa acgctgctct
      961 ttctcctgcg ccttttctgc ctgctgtctc tgcaactgcg ccagttgtgt ttccagtagg
     1021 gtgatttgct gttgaatcaa ttcggcctgt ttttgcttgt cttcagcact gccgctagtg
     1081 tctacaatct ctttaatttg ttgagtgagt ttgatgattt gctgacttat ccgcgcgatt
     1141 tgtgaagcaa catcgccacc ttgtgatgcc ctgttactac tctgtgtttg tatggaagga
     1201 attgaagttg aagagatagt ggccattgtt cagtcctcgt cactgtacga ggtaatatcg
     1261 gtagaagaac aaggaacttg agaagaaaaa tcaatcttac aaatcgcaga tagcaaaaaa
     1321 gcgcctttag ggcgcttttt tacattggtg ggtcgtgcag gattcgaacc tgcgaccaat
     1381 tgattaaaag tcaactgctc taccaactga gctaacgacc cgaagtggtg ggtgatgacg
     1441 ggatcgaacc gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc
     1501 cacttcggta cttcacattt ttacaagaaa tttagctggc gagaaagtgg tgggtgatga
     1561 cgggatcgaa ccgccgaccc cctccttgta agggaggtgc tctcccagct gagctaatca
     1621 cccacttctc aatttcttgc tacacggcgg agactacata aagtagttgg tgggtgatga
     1681 cgggatcgaa ccgccgaccc cctccttgta agggaggtgc tctcccagct gagctaatca
     1741 cccccgctgt gtggagtcgc attataggga gagttcaaaa tgagtcaacg cattttctaa
     1801 agaaattgtt cgttcgtcgt aaatttaagc aagatgatcg caaaacagac cgtgttgcgc
     1861 aatttgtcaa cgaaaacaat aatgcgtaag gtagaaaccc gaactacatt gaggaatcag
     1921 gcgggagtga tagaatatcg cccactcaat ttttccagga tttgccggtt gtcggcatct
     1981 ttctaaacgt aaggccattt catgaaaatc aaaactcgct tcgcgccaag cccaacaggc
     2041 tatctgcacg ttggcggcgc gcgtactgct ctttactcct ggctttttgc acgtaaccac
     2101 ggcggtgagt tcgtgctgcg tattgaagac accgatcttg agcgttccac gccggaagct
     2161 atcgaagcca ttatggatgg catgaactgg ctgagcctgg agtgggatga aggtccgtac
     2221 taccagacca aacgttttga tcgctacaac gcggtgatcg atcagatgct ggaagagggc
     2281 actgcttata aatgctattg ctctaaagag cgcctggaag cgctgcgcga agagcaaatg
     2341 gcgaaaggtg agaagccgcg ttatgacggt cgctgccgcc acagccatga gcatcatgct
     2401 gatgatgaac cgtgtgttgt acgttttgct aacccgcagg aaggttctgt tgtttttgac
     2461 gatcagatcc gtggtccgat cgagttcagc aaccaggaac tggacgatct tattatccgc
     2521 cgtaccgatg gttccccaac ctataacttc tgtgtggttg tcgatgactg ggatatggaa
     2581 atcacccacg ttatccgtgg cgaagaccat atcaacaaca cgccacgcca gatcaacatt
     2641 cttaaggccc tgaaagcgcc ggtgccggtt tacgcgcacg tttctatgat caatggcgat
     2701 gacggtaaaa aactgtccaa acgtcacggg gcagtcagcg taatgcagta tcgtgatgac
     2761 ggttatttgc cagaagcact gctgaactat ctggtgcgtc tgggctggtc ccacggcgat
     2821 caggaaatct tcactcgtga agagatgatc aaatacttca ctttgaatgc cgtcagcaaa
     2881 tctgccagtg cgttcaacac cgacaagctg ctgtggctga accatcacta cattaacgcg
     2941 ctgccgccgg agtatgttgc tactcactta cagtggcaca ttgagcagga aaatatcgat
     3001 acccgtaacg gcccgcagct ggctgatctg gtgaaactgc tgggcgaacg ctgcaagacg
     3061 ctgaaagaga tggcacagag ctgccgttat ttctacgaag attttgctga gttcgatgcc
     3121 gacgccgcga aaaaacatct gcgtccggta gcgcgtcagc cgctggaagt ggttcgtgac
     3181 aaactggccg cgattactga ctggaccgct gaaaacgttc atcacgctat tcaggcgacg
     3241 gcggatgagc tggaagtggg tatgggtaaa gttggtatgc cgctgcgtgt cgccgtaacc
     3301 ggtgcggggc agtctccagc actggatgtt accgttcacg caattggtaa gacccgcagt
     3361 atcgagcgta tcaacaaagc gctggatttt attgctgaac gcgaaaatca gcagtaatta
     3421 gcgtgtaaaa agataaacgg caggagataa tatcctgccg ttttttattt atgctgattc
     3481 gtcaatatcg agacgggcga ggaaattgcg aattccttca cgtgacaaaa attgcgcgac
     3541 tttttcctgc tcaatgtctg tcatattatc tatcgcgttg atgatttgtc gatacgccgg
     3601 ggcgcgtgct ccaggagcgt attcagcaat tctctcttca gcttccatca acattggtgt
     3661 gttatggaag gctggcgtgt tctgaataaa ctcgcaaact tgcgtatcga ccagaataag
     3721 tctggcacga ccgcctttca cgccgggaaa tttttccgtt ttccagcctt tttctctgat
     3781 ccaacggtta acagtttgtt tagccacacc gaggcattca gccagttctt cggtggtcat
     3841 tttattgcgt aatcttttca tattatttgc tatcgcgaat ccctaagcgt tgtaacaatc
     3901 cgataatccc ttcccgcaga agcaaggatg taaactgttt ttgttcaact ggcgtcatct
     3961 ctttcgccag tgtcaccagc aatacttcaa gcggcgcatc accagaaagg gcaggcgctt
     4021 ctccctgacc ttctggacgt tcagcattgc gaatatattc acgaacctgt tcattgacgt
     4081 gaaccagtcg ggctttgcca ccctggacgc caggttttgg tgacgttgtc cagccttcct
     4141 tgcgtaccca tttattaatg gtctggcggc tatagccggt cagtctggca agttcatctg
     4201 gtgtcatcct ctccttgaac ataacaattc cctgaatgta tgtgggtcta ttagtagttc
     4261 attttataac accactttac aggaaaccgt gacatgaata actactgaat gcgagcggtt
     4321 ttccgaaatt gttcatttgc ctgatttttc agcgattgaa actacacgtt gcaatttgcc
     4381 gttgacacat tcgggcggaa ttcatatgat gccgcccgtc aactcgacaa gctttacgtg
     4441 acggggctat agctcagctg ggagagcgct tgcatggcat gcaagaggtc agcggttcga
     4501 tcccgcttag ctccaccaaa ttttgcaccc agcaaacttg gtacgtaaac gcatcgtggg
     4561 gctatagctc agctgggaga gcgcttgcat ggcatgcaag aggtcagcgg ttcgatcccg
     4621 cttagctcca ccaaatttcc aaccctcgct gcaaagcggg ggttttttgt ctctgctttt
     4681 tgccgctttt gtaatacagt ctacgtccgg gttagtgccg cctggtgaaa gcatcattgg
     4741 atgaaaaaac ggcaacaggc tggccccctg tttgcttcgc gatgcgaata aacttattat
     4801 ttgtgtgcct gaaaaccccg atcagtgaga gtagtgtact catgtttgtg gagcataacc
     4861 tgataaaaaa tatcaagata ttcacactag cgtttacgct caccgtggta cttattcagc
     4921 tatcccgttt tatttcgcca cttgccatta tccattccag ttatatcttt ctggcgtgga
     4981 tgccactgtg cgtaatgctg tcaatcttgt ttatctttgg ctggcgcggt gtcgttcccg
     5041 ttttatgcgg gatgttttgc accaatctgt ggaactttca tctctctttt ttacagactg
     5101 cggtcatgct cggtagccag acgtttgtcg tgttgtgtgc ctgcgcaata ttacgctggc
     5161 agctggggac gcgttggcgt tatggattga ccagccgata tgtctggcaa cgtctgttct
     5221 ggcttggttt ggtgacgccg atcggcatca aatgcagcat gtatcttgtg gggagcttct
     5281 ttgatttccc gctaaagata tccacctttt tcggcgatgc ggatgccatt ttcacggtcg
     5341 ttgatttgct aagccttttc accgctgtgc tgatttacaa catgcttttc tactatctca
     5401 ctcgcatgat tgtaagtccc cactttgcgc agatattgtg gcgcagggat atcgctccgt
     5461 cgttgggcaa agagaaacgc gcatttacct taagctggct ggcagcgtta agcgtgctgc
     5521 tacttctgtt gtgcacacct tatgaaaacg actttattgc cggttacctg gtacccgttt
     5581 tcttcatcat ctttaccctc ggggtcggta agcttcgcta tccgttttta aatctcacct
     5641 gggctgtttc aacgctttgc cttctgaatt acaaccagaa ctttttgcaa ggggtggaaa
     5701 ccgaatattc gctggcattt attcttgcgg tgctgatttc ctttagcgtt tgcctgctct
     5761 atatggtgcg catttatcat cgcagtgaat ggcttaatcg ccgctggcat ttgcaggcgc
     5821 tgacagatcc gttaacgctc ctacccaact ttcgtgcgtt ggaacaagcg ccggagcaag
     5881 aggcgggcaa gagtttttgc tgcctgcgca ttgataatct tgagtttatg agtcgtcatt
     5941 acggcttaat gatgcgcgtt cactgtatcc gctcaatttg ccgtacgctg ctgccgttga
     6001 tgcaggaaaa cgaaaagttg tatcaattgc cgggtagtga actgctgtta gtgctgagcg
     6061 ggccggaaac ggaagggcga ctccagcata tggttaacat cctgaatagt cggcaaattc
     6121 actggaacaa taccgggctg gatatgggct atggtgctgc ctgggggcgt tttgatggaa
     6181 atcaggaaac cctgcaaccc ttgttggggc agttaagctg gctggcggag caatcctgcg
     6241 cacatcatca tgtgctggcg ctggagagca gagaggagat ggtttccggg cagaccacta
     6301 aacaggtgct attgctgaat accattcgca cggcgttaga tcagggtgat ttgctgctct
     6361 acgcccagcc aattcgcaac aaagagggtg aaggttatga tgagatcctc gcgcgactga
     6421 aatatgacgg cggcattatg accccggata agtttctgcc ccttattgct cagtttaacc
     6481 ttagcgcgcg ttttgatttg caagtgctgg aatccttgtt gaagtggctg gcaacacacc
     6541 cttgcgacaa aaaaggaccg cgcttttcag tcaatttaat gccgctcacg ctgctgcaaa
     6601 agaatattgc cgggcggatt attcgtctgt ttaagcgtta tcacatctcc ccgcaggcgg
     6661 tcattcttga gatcaccgag gagcaggcgt tttctaacgc agaaagcagc atgtacaaca
     6721 tcgagcagct gcataagttt ggtttccgga ttgcgattga tgactttggc accggatatg
     6781 ccaactacga acggttaaag cgtttgcagg ctgatatcat caaaattgat ggcgtctttg
     6841 tgaaagatat tgtcacgaac acgctggatg cgatgattgt gagatcaatt accgatctgg
     6901 cgaaagcgaa gtcattgagt gtggtcgcgg agtttgtcga gacgcaacag cagcaggcgc
     6961 tattgcataa gctcggggtg caatatctgc aagggtattt gattggtcgc ccgcagccat
     7021 tagctgatta acaggcgtaa aaaaaccggg gaatt
//
LOCUS       DAPHBJ010000217         7048 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.219, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000217 DAPHBJ010000000
VERSION     DAPHBJ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.219"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..467
                     /gene="hlyE"
                     /locus_tag="RHZ76_003980"
                     /pseudo
     CDS             1..467
                     /gene="hlyE"
                     /locus_tag="RHZ76_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353800.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            421..852
                     /locus_tag="RHZ76_003981"
     CDS             421..852
                     /locus_tag="RHZ76_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin E"
                     /protein_id="HDV2814521.1"
                     /translation="MIFQKKNSYFQSQVDKIRKEAYAGVVAGPFGLIISYSIAAGVVE
                     GKLIPELKNKLKSVQNFFTTLSNTVKQANKDIDAAKLKLTTEIVAIGEIKTETETTRF
                     YVDYDDLMLSLLKEAAKKMINTCNEYQKRHGKKTLFEVPEV"
     gene            complement(1059..1505)
                     /locus_tag="RHZ76_003982"
     CDS             complement(1059..1505)
                     /locus_tag="RHZ76_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HDV2814522.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1597..2256)
                     /locus_tag="RHZ76_003983"
     CDS             complement(1597..2256)
                     /locus_tag="RHZ76_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDV2814523.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            complement(2328..2621)
                     /locus_tag="RHZ76_003984"
     CDS             complement(2328..2621)
                     /locus_tag="RHZ76_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HDV2814524.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            2863..3264
                     /locus_tag="RHZ76_003985"
     CDS             2863..3264
                     /locus_tag="RHZ76_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HDV2814525.1"
                     /translation="MKIKSISKAVLLFALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(3367..3735)
                     /locus_tag="RHZ76_003986"
     CDS             complement(3367..3735)
                     /locus_tag="RHZ76_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814526.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGNDAGGLQSPACGVVCDPYIC
                     VSSDGISPALTRKYLGEKAAENLQSLQGYDPSEFTFSNGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            4255..4950
                     /gene="minC"
                     /locus_tag="RHZ76_003987"
     CDS             4255..4950
                     /gene="minC"
                     /locus_tag="RHZ76_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDV2814527.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPTPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4974..5786
                     /gene="minD"
                     /locus_tag="RHZ76_003988"
     CDS             4974..5786
                     /gene="minD"
                     /locus_tag="RHZ76_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDV2814528.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            5790..6056
                     /gene="minE"
                     /locus_tag="RHZ76_003989"
     CDS             5790..6056
                     /gene="minE"
                     /locus_tag="RHZ76_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDV2814529.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(6428..6771)
                     /locus_tag="RHZ76_003990"
                     /pseudo
     CDS             complement(6428..6771)
                     /locus_tag="RHZ76_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(6806..6925)
                     /locus_tag="RHZ76_003991"
     CDS             complement(6806..6925)
                     /locus_tag="RHZ76_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDV2814530.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(6886..>7048)
                     /locus_tag="RHZ76_003992"
     CDS             complement(6886..>7048)
                     /locus_tag="RHZ76_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814531.1"
                     /translation="TITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQRTACKKCLF
                     LMRDIIISS"
BASE COUNT         2015 a         1681 c         1551 g         1801 t
ORIGIN      
        1 tcgaaaccgc agatggagca ttagatcttt ataataaata tctcgatcag gtcatcccct
       61 ggcagacctt tgatgaaacc ataaaagagt taagtcgctt taaacaggag tattcacagg
      121 cagcctccgt tttagtcggc gatattaaaa ccttacttat ggatagccag gataagtatt
      181 ttgaagcaac ccaaacagtg tatgaatagt gtggtgttgc gacgcaattg ctcgcagcgt
      241 atattttgct atttgatgag tacaatgaga agaaagcatc cgcccagaaa gacattctca
      301 ttaaggtact ggatgacggc atcacgaagc tgaatgaagc gcaaaaatcc ctgctggtaa
      361 gctcacaaag tttcaacaac gcttccggga aactgctggc gttagatagc cagttaacca
      421 atgatttttc agaaaaaaaa cagctatttc cagtcacagg tagataaaat caggaaggaa
      481 gcgtatgccg gtgtcgtcgc cggtccattt ggattaatca tttcctattc tattgctgcg
      541 ggcgtagttg aagggaaact gattccagaa ttgaagaaca agttaaaatc tgtgcagaat
      601 ttctttacca ccctgtcaaa cacggttaaa caagcgaata aagatatcga tgccgccaaa
      661 ttgaaattaa ccaccgaaat agtcgccatc ggggagataa aaacggaaac tgaaacaacc
      721 agattctatg ttgattatga tgatttaatg ctttctttgc taaaagaagc ggccaaaaaa
      781 atgattaaca cctgtaatga gtatcagaaa agacacggta aaaagacact ctttgaggta
      841 cctgaagtct gataagctat tattctctcc atgtactcaa ggtataaggt ttatcacatt
      901 taaaaacaca gacgtttttt aaagattgag agttacctgt tgataataag gtatcgggta
      961 actctcatta tgacagagcg ctttcaaaaa cagggctacg aaatggcgag catccagacg
     1021 tatattaaga aagcccccat caacatctat taaatttatc actgcgccca gtcaggttta
     1081 tttaagatat gatcctgcca gtcaatcact tctgattctt tcactgcgat atgacgcaca
     1141 gagatacgtt caccatgcat tgccgctttc gaacccgtaa gtagcggatg ccacgcaggt
     1201 aaatctttac cttccgccag caaccgataa gcgcaggtca ttggcagcca ttcgaatgtt
     1261 ggcagatttt cacgggttaa tttaatgcag tcgggttcaa actcgaaacg acgttcgtag
     1321 ttccgacatt gacaggtttt aatattgagc tggcgacagg cgacgttagt gaagtagatt
     1381 tcgtcggtgt cttcatccat cagtttatgc aggcaacact gaccgcaacc atcacacaac
     1441 gactcccatt ccgcatcgct catttcgtcc agggttttac tttgccagaa aggtacatcg
     1501 ctcatcaggt gttccgccat tacgttaaaa cgcaccttat aaccagtctg gcacagcgat
     1561 gcaagttttg ccgccgcttt caggcggcaa aaagtattac aaaacgcgag ttgtcaaaga
     1621 atgcccatcg aaagtgactg tcagctcatc accgctttgc aacgggccga cgccatcagg
     1681 cgtgcctgtc aacacaacgt caccggcctt gagggtaaaa aacttgctca tataagcgat
     1741 cagcggaacg attttatgga tcatgtccgc agtcgtacct tgctggcgtt gctcgccgtt
     1801 tacgctcagg cccagcgttg tattttgcgg gtcgccggta aattccgccg cgggaataaa
     1861 cccggaaagc ggacaagagt tatcaaacgc tttagccttt tcccacggct gcccggcttt
     1921 cttcattttt ccctgaacat cacgcaacgt cagatcgagc gccacgccat aaccggcaat
     1981 ggctttgcgg acatgctctt ccgtagcctg acgcagcgtc gcgccaatca acaccgccag
     2041 ttcgacttca tgatgaactg aaccgaaatc ggatgggatc gccaatggct gccgcagatc
     2101 gcacagtgcc gtttctggtt taataaacag cactggctct tcgggcactg cgctgcccat
     2161 ctctttaata tgtttggcat agttactgcc aacacagact actttactca ccggataatc
     2221 cagcagcgca ccttgccagt tgtgatgttg atacataacg ttcccctcac cttatcgtgg
     2281 atatgaaaac ccgcgccgac aaagataatg ccaccgccgg atatcggtta tttgttagtg
     2341 tcgtctgttt tctgccccat gacggaaaga tgttgcttca gcaaatcttc gggtggtggc
     2401 ggtaactgca aatagtaacc ttgctcggtt aatgccagtt taaccttttc aatatcagca
     2461 ttgaccagtt tcttccgccc atccaacggc agaatcatcg ctaactgagg ctgaccaaaa
     2521 cctttcatca gttcctcagg aacacgcgaa aaatcgtctt ttttttcgac atataaatag
     2581 gtctggtcac gcttgctgct tcgataaatc acacaaaaca tacttttact ctttaagatg
     2641 ccaggttttg gcataagcta acatcaatga aacccgcgcc acaagccagc cagctgtaga
     2701 tttctgcggt ttgttgagtc catgcccctt tataagtaat ccccccggca aaaaatgccg
     2761 cgccgggatt ttaccgaacg cagaaaagcc actggaattc tcccgtaagg gattgttata
     2821 ttataacagt tcatcgtact cattctgaac aggagactac caatgaaaat caagagcatc
     2881 agtaaggctg tattgctgtt tgcactgcta acctccacga gctttgctgc gggtaagaat
     2941 gtcaatgttg agttcagaaa aggccatagc agcgctcagt actccggcga aataaaggga
     3001 tacgattacg atacatatac cttctatgcc aaaaaaggtc agaaagtaca tgtgagtatc
     3061 tctaatgaag gcgctgatac ttacctcttc gggccaggaa tcgatgattc tgttgattta
     3121 tccagatatt ccccagaact cgatagtcac ggacaatact cactccctgc ttctggcaaa
     3181 tatgagctgc gagtgctgca aacccgaaac gacgcccgta aaaacaaaac taaaaaatat
     3241 aacgtcgata ttcaaattaa gtaaatatca accgacgggg actctccccg tcataaacgt
     3301 taacgcccca ctcctctctg tcgcttaaga ggttaatgac gaatagaaca taatgtaaac
     3361 aagacatcac tcacgacaca taaataacat ctttgtggtg gtttgattga tttttccact
     3421 ccgcttacca tccacaccaa aataacgatc atcacgacat aatttttctt taacatcgca
     3481 aaatacaccg ttagagaatg taaattcgct gggatcgtag ccttgtaatg attgtaagtt
     3541 ttcagcggct ttttcgccga gatatttcct tgttaacgct ggggaaatgc catctgaact
     3601 cacacaaata tacgggtcgc aaacaacgcc acacgcagga gattgaagcc cgccagcgtc
     3661 atttccagac gccattgatg agaacatcat ccccacacct gacaatccga tataaaaaat
     3721 acgcatcata ttcatagcac atcctgataa tccaaccgta caacacttat gctcatttca
     3781 tcatcaatcc gtaatcaaaa ccgctgacct ggacgagtta cgatgacctg acagaagtat
     3841 cagacgtctt ctgcggtttc tatgatagag caattctgtg gcggatatta agataacggg
     3901 tatctgttct ttaatgatgc tgcaaaaaat ggaaagttaa gaaagcctgg cgtggtgaaa
     3961 aaatacactc gcctcaacac tggatattta aacaggtctg aagatgctgt cacgattagg
     4021 gtgcggaaaa aggaagcgta aataagaaca atcaaaagta cgacggcaat gggttgattg
     4081 acaagggtat tttttaagct atgaatcagc gccatttatc acagaataga cttttactct
     4141 gaataaatgg gagggtgact tgcctcaata taatccagac tataacatgc cttatagtct
     4201 tcggaacatc atcgcgcgct ggcgatgatt aatagctaat tgagtaaggc caggatgtca
     4261 aacacgccaa tcgagcttaa aggcagtagc ttcactttat ctgtggttca tctgcatgag
     4321 gcagaaccta aggttatcca tcaggcgctg gaagacaaaa tcgctcaggc ccccgcattt
     4381 ttaaaacatg cccccgttgt actcaacgtc agtgcactgg aagacccggt aaactggtca
     4441 gcgatgcata aggcggtttc ggcaaccggt ttgcgggtta ttggcgtaag tggctgcaaa
     4501 gatgcgcaac ttaaagccga aattgaaaag atggggctgc ctatcctgac ggaaggaaag
     4561 gaaaaagcgc cacgtccagc tcccacaccg caggctccag cgcaaaatac aacgccggtc
     4621 acaaaaacgc gtttaataga taccccggtg cgttccggtc agcgtattta tgctccacaa
     4681 tgtgatctga ttgttacaag ccacgttagc gctggggccg aattgattgc cgatgggaac
     4741 attcatgtct atggcatgat gcgcggtcgt gcgctggcag gggcaagtgg tgaccgggaa
     4801 acgcaaatat tttgtacgaa cctgatggcg gaactggtgt ccatcgcagg tgaatactgg
     4861 ctgagtgatc aaatcccagc agaattttat ggcaaagcgg cgcgactgca gttagtcgaa
     4921 aacgctttga ccgttcaacc gttaaattga tcccttttta acaaggaatt tctatggcac
     4981 gcattattgt tgttacttcg ggcaaagggg gtgttggtaa gacaacctcc agcgcggcca
     5041 tcgccactgg tttggcccag aagggaaaga aaactgtcgt gatagatttt gatatcggcc
     5101 tgcgtaatct cgacctgatt atgggttgcg aacgccgggt cgtttacgat ttcgtcaacg
     5161 tcattcaggg cgatgcaacg ctaaatcagg cgttaattaa agataagcgt actgaaaatc
     5221 tctatattct tccggcatcg caaacacgcg ataaagatgc cctcacccgt gaaggggtcg
     5281 ccaaagttct tgatgatctg aaagcgatgg attttgaatt tatcgtttgt gactccccgg
     5341 cagggattga aaccggtgcg ttaatggcac tctattttgc agacgaagcc attattacca
     5401 ccaacccgga agtctcctca gtacgcgact cagaccgtat tttaggcatt ctggcgtcga
     5461 aatcacgccg cgcagaaaat ggcgaagagc ctattaaaga gcacctgctg ttaacgcgct
     5521 ataacccagg ccgcgtaagc agaggtgaca tgctgagcat ggaagatgtg ctggagatcc
     5581 tgcgcatcaa actcgtcggc gtgatcccag aggatcaatc agtattgcgc gcctctaacc
     5641 agggtgaacc ggtcattctc gatattaacg ccgatgcggg taaagcctac gcagataccg
     5701 tagaacgtct gttgggagaa gaacgtcctt tccgcttcat tgaagaagag aagaaaggct
     5761 tcctcaaacg cttgttcgga ggataagtta tggcattact cgatttcttt ctctcgcgga
     5821 agaaaaacac agccaacatt gcaaaagaac ggctgcagat tattgttgct gaacgccgtc
     5881 gcagcgatgc agaaccgcat tatctgccgc agttgcgtaa agatattctt gaggtcattt
     5941 gtaaatacgt acaaattgat cctgagatgg taaccgtaca gcttgagcaa aaagatggcg
     6001 atatttctat tcttgagctg aacgtgacct taccggaagc agaagagctg aaataagccc
     6061 gctgtaaaag cgcatttatc ttcaaggcag agttatctct gccttgagtt tttcatccct
     6121 ctcatccacg ttgtggtaaa gcggcgagta ttcttgctga tactcctcat tgctatttcc
     6181 acctccccct ttacacctta aggctgttcc cacgcacaaa acaaaatgtt tatgcctggt
     6241 tgagtaaata accttattgt tagaatggat atactgggag atgataaccc cgcaatttca
     6301 ttattcattg ttctcccata ggatgaggtt tcccgccgta actggcgggc tttttttgcc
     6361 ccagaatttt ccctttcaac atcctgtaaa cgaaaactgc gccgaagcgc agtttaatcg
     6421 ttgatggtca gaacgaccag ttcacaccag ccaccgcgtt ccacggggat tccacaccgg
     6481 caccatggct ataccccacc ccgagatgtc cgctaaacgt actgctgaat gaggctttaa
     6541 tacctgcctg gtatattcca cgtctgcccg acaaatcatt gacgaaatta ccgtcattat
     6601 tcaccttcac ccggttatca tcgacaaatt ctttgcgcac agccgccttc agccagggtt
     6661 catttccata ccgttcccca ggcgcatgtt gtaactcagc gttgcaccca gttcacgata
     6721 tatactgcgg gtatcgactg atttcgattc catgccattg gataaatgat agtagcgtct
     6781 aagtgaccta agctctgctg caacatcagc ccgcaggcac cagaccaggg gattcatcct
     6841 gaagagacag cgcaggtgta ttgtgttcac cgctcaccaa agacatcatg atgaaatgat
     6901 gatatcccgc ataagaaaga ggcatttttt acacgcagtg cgctgaagtg tagttggata
     6961 aaaaatcact cttttcagat aatactgtcg tacgcttatc tttcgacaat gaggccgtcg
     7021 gcaaaataaa atgactaatg taatcgtt
//
LOCUS       DAPHBJ010000218         7028 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.220, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000218 DAPHBJ010000000
VERSION     DAPHBJ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.220"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(72..2855)
                     /locus_tag="RHZ76_003993"
     CDS             complement(72..2855)
                     /locus_tag="RHZ76_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDV2814532.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYVFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLPTRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(2912..5284)
                     /locus_tag="RHZ76_003994"
     CDS             complement(2912..5284)
                     /locus_tag="RHZ76_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDV2814533.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYLKGKGRLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFANQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIMLGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLQQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5322..7007)
                     /locus_tag="RHZ76_003995"
     CDS             complement(5322..7007)
                     /locus_tag="RHZ76_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HDV2814534.1"
                     /translation="MITIPITLRMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILA
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
BASE COUNT         1818 a         1614 c         1585 g         2011 t
ORIGIN      
        1 tataaaatta ttacataaaa atagcgaata ttgctaaaat ccccgccaac gatgtgttga
       61 cggggctgtt attattttgg caataatact ccggtataag tatttaccgg atgagaaaga
      121 tattgtttaa cggcagtgtt aacattctct accgtcattt gtttcaacaa ttgctcctgc
      181 tcagtccatg ctgcaggatc gtcatattga ataagactat ttacaatggt gttcgctaat
      241 tgttgaacgc tacgctgttg gatatcgagg ctgcgctgaa cgttttgctg gtattcattc
      301 agttcttgct cactgatccc tttagccaga cgcttaacca tcacttcatt cgctaacgtt
      361 aacagttcat catgtcgttc tggttgacaa gtaaaagcca gcaaatgact gatatctttg
      421 gcctgaggat caaccgagag gcgagaagaa acgctgtatg ccccagatgc ctgttcacga
      481 atattaacgc gtagatcttt tgccagtgcg acgttaaaag catcgagcgc catacgcgtc
      541 ggcagattaa caggtgtccg ggaatcataa cgcttccact gtgaaacctg tgccacaggt
      601 tcattttgtt cttttacagt aaccgatgcg ttgtccgtcg cgcgagttaa tggtttacct
      661 gcggctaatg gcgaatcaga gtgtttgatt gatcctaagt aacgcgtaat taacgccacg
      721 agtttgtctt ctgcgacatt accgacaatg acaaacgtga tatccgctgg agatgaaaac
      781 aattggcgat cggcagccag cgcatctgcg gcagtaaact gtgcaatctg attttcttgc
      841 agtaatttcg tgcggtcatc agcatagcgc gtctcataca tctgctgggc gaatttctcc
      901 gctggacgct ggtcgagcgt tttcaacgcc tgaatttgag cattttgtag cgatgcccaa
      961 atattatcgt taatcgtgct gtgggtgatt cgctggttaa tcaactgaaa accaggttca
     1021 gggttattag tccgcgcgct aacggagagc aacgtattca tgccactgac tttactgctc
     1081 atggttaccg aattttccgc actccagcgt ttcaggctag aagaggagag ttcgccaacg
     1141 ccgcttccgc taactgcttt atttgccagc gcgataagtg atttttgctg cgcagggaaa
     1201 cttaaatcgc ctttattcga tacggcaata atttgtagct tttgctcttc acccgcggat
     1261 tttgccagaa taaccctggc accattggaa agtgttaatg atgtcagatt ctccgccagc
     1321 gtttctttgc tgctaatttc cgcctgtggg tctgcgtcta ctgttaacga taaatttctg
     1381 cctgggaaga cgtaagccgc cagctttttg ttggcgtact ccttttccag cgccaggata
     1441 gccgcaggag acaatgcttt tttggcggca acctcattgt ttaccatttg ctcccaaaat
     1501 gcgtcctggt tctttcttaa ctgctgccat ttttccgcca gactttgcac ggtaatttgc
     1561 tgccacagac gtttcgaaag ttgatatgtc tcttccggcg acaagaacgg cgtattattt
     1621 aatgagctgg atgccaggcg actggtcagc atacgtaaat cacgttcagc ttgctgatca
     1681 accgcatttt tcagccaggt gaggcgggta gatttgacat catcgagttc ttcagcagaa
     1741 aaaccatgct gatcaatggt tgccaactct gccattaatg cattcgcagc atcctgcata
     1801 ttatcgtctc gtgcatttac acggaaaaac agcgactgat aatcgggtgc aattttaacg
     1861 ctgcgcgcag tgccgccaga aatagtcttc aactcgcccg actgtatgcg ttcctgcaga
     1921 cgttgattga acagctgaac taacatgctc cattcagctt gttcgacaaa gctttgctca
     1981 tcgttcactt gtaccattgg caggcgataa tagagtgcga tgccgttcac ccggttttct
     2041 ttatcattaa tgatattaaa gcgcaggtgg ttttcggctt ttgtcggcca gacgcgattt
     2101 tcagctgctt tgttagccgg aagcttactt aaattatcct ttatcagcgc cagcgcttct
     2161 ttactgtcga tatcgccgac cacgataaag gtcatattat ttggttgata ccagcgttga
     2221 taaaattggc gcaattgtgc cggtgtgacc gtggcgacag tatccatcag gccgataggt
     2281 tcacggtcta aattacgggt atttgccagc aggaaagggc ggcgcgcctg agaggtgcgc
     2341 catttcgcat cctgatgggc acgccattcc tcagtaatta cgccacgttc agcgtctact
     2401 tcgagttttt caaaggtggc ggcattactc cattcactga agattgccat cacttgttgc
     2461 agattttgtt tctgcgtagt cggcaaactc acctgataca ccgtttcgtc atagctggta
     2521 taggcattaa catcgcgacc aaaacgcagg cccattgact caaatgtttc gatgacttta
     2581 ttacccggcc atgtttttgt gccgttaaac atcatatgtt ctacaaaatg agccacgccg
     2641 cgctcattgt cttcttcctg caatgaaccg gtatgaattt gcagccataa atttacctga
     2701 tcctttggat gagcatgcgg ataaatcata tatcgcaagc cattgtccag ttgcccggta
     2761 attaactttt catcctgcgg taaggcggcg gcaatcagcc gcccggggag caacagagtt
     2821 gccactaacg tcagtaagaa acagaggttt ctcataatta tctccatgcg aaaaccgggc
     2881 gaatttaccc ggttaagtaa aatccgaact attaaaattt catgctgaca tcaagccaga
     2941 aagtacggcc actggcgtag gtcgccacac cggtgttcgt tgtatcaaca gcggttttgc
     3001 tatccagtac attgagaata tcggcactga tcgtcaggtt ttgttgttgc agaaattgtg
     3061 gcgtccagga caagcgggtg tcccaggtca ggctgctatc cagtttttcg tcaacatact
     3121 gcttgtaatc tgaatattcg ctgatgtatt gcgcatttgt cttaccaagc ataatgcgag
     3181 ctttacgcgc ttcttgccag gccagcgtat tcgcccacac taacccgctc ggttgatgcg
     3241 tgaaatccat gtttaaggag atctttaatg ggttattaaa atctgccact gggacgctat
     3301 cgtaagagac cagattaccg ttataaacca cctggttatc accggtattg ctctcctcat
     3361 aaccattgtt taacgacaag ttgcccttgc tcttgatata gctaaagaca atttgtgggt
     3421 taatatctac ctggcggata tgcaggggtt cggccagttc aaaactgagg ctgaacgaat
     3481 gcgttttggt tttgccatcg ttgttatatt cagtaatagt ggttttagtc gcgctgtcgg
     3541 tacgactgct tttgctgatt tgatcatgcg cttcacggta aacatagttt gcgcgcgcaa
     3601 taacgttctt accgattttc tgctgcaatc ccattgccag ttcatcgtta taaggcgttt
     3661 tcaaatcctg ataacgcgtc agagttttat tacctgatac cgattccgtc cagctattgc
     3721 ggatatcacg taatcccata tcaagaatat tcccgccata gtaacggtta taacctgctg
     3781 taatcattga ggtttgatta gcaaaaatat cccattccgt cataaagcgc ggggagatat
     3841 tgtggtttga cagatagttg tcatagtcat accgcacgcc gggcattaat gacacattac
     3901 gccagctaat gcgatccgcc atatacagcg tgtagttgtc aattcccagg cggcctttac
     3961 ctttaaggta aatggtatgg ttagttttct ttccggcagc attaatcaca taggattcag
     4021 actggttatg gcgttcagtc cacgcatcgg aatagatgta ttccgcgccg aagtagggtt
     4081 gatgcgaaac attacccacg gcgaattttt gccagtccag gcgtgttttg aaggtgtaat
     4141 tatctacagc ctgggaaatg tgtcctaatc cgccacgggt gcaacgccct gtaatatcac
     4201 catatgtaca tgaaagttcg gtgtaccaga tgtcatgatc gtgacgggta taatcactaa
     4261 tatgatccca accaacggtg gtacgcagtt tggcccatgc gagctgcgta tccatatccc
     4321 atgccagacc ataggattta ttacccattt cgcgatcaga ctgcgggaag gtgctggtat
     4381 tataatcacg gctggagccg gtatatttta aggttaaatc gtgggtaaag cggtcgctgg
     4441 caaaccaggt aaatttgctc aatgcagtat cgataacgtt tttatactgt gcccgaccgg
     4501 cgacaatgcc gtcattcgaa acataatccg cgcgggtgat atcagactgg cggcgcgata
     4561 aaccggcggt aacgccgaag ttatcagcga gttcctgatt aaacgacaag gtataaaagt
     4621 tctttttaaa atctggggag taataagtac ttcctgaaga gccttgatta aatgcgctct
     4681 tgttattctc atcgatatgc gatgttaacc agtccgaacg cgtagtgcga taacccaatt
     4741 tcaccttgct atcatcagcg ttgaagcgtt tgatctttgc atcaattacc ccgccattga
     4801 agcgaccaaa ttcaaccggc acaaaactgt cataaagcgt cacattgtcc agtaagctga
     4861 catcaagata atacccctgt gacatcccgc taatattggt tgcactactg gcatcggact
     4921 cattcgctgg gttcaggtta ttagttgcac taataccgtc aatcaaatag gcattctggt
     4981 agggcgacgc accgtgaata gagattttct cagggcgaat atctccctgg ttcaacgagg
     5041 tactttgcgt tgaatccatg cgtaccgcag ggttggttct cagcagatcg ctgatattgc
     5101 cattcccggt cggtaaacgc tcgatgcttt cactggtgta atgcgtgttg ccgtttacgg
     5161 gtacagggac aggagcatag accgtcatct cttcaaaaaa atgcatgtac atgtttttgt
     5221 catcgacggc ctgcgccaca tcagcgccac ataaaatgac gccaggaata agaactcgct
     5281 tcatttattt atatctccgt acagcagggc ttttattgtt tttataaaac cgcgctaatg
     5341 tcacaaatat catcggccag gttccagaca ccgggttgat gtgtaaccat aatgacgccg
     5401 cttgtgggta gtttttcacg cactaaacgc agtaagcgga tagcctcttg ttcctcaaga
     5461 tgagaggtag tttcgtcaag aaatatccat ttcggacgtc gtaaaattaa tcgtgccagg
     5521 gcgatacgtt gtttttcgcc gctggaaaga atatctcccc agcgatcgtg gtcatgaata
     5581 cgcgcagcca atttcccaag accaacctga tgcagtactt cgctcaacga tttatcgtct
     5641 acgggcaggg gaagtgcttt acaaataatc tctttcagta agccggtttt gattaacggt
     5701 gtttgtgaca cataccaact gtcagcagga gaagaaatat cacctttaaa ccacggccag
     5761 cagtgggata atgttttaag cagtgtggtt tttcccgcac cagagtagcc tttcagtaat
     5821 agccatttgc ctggcgaaac atgaaagttc aagttctcta atatgatctt attatcaggc
     5881 gtacgaatac tcgcatcagc cacttgtacc gcatgttggc aatttttagg cttattcgta
     5941 gggcgctgtt cagtgagttg atggaactca tacaagcgat cgataaccgc agccagttca
     6001 gcaagttcgt catatttata aataaaccag cttaaattgt tcgataccag cataaatgcc
     6061 tggcgcgatt tcatcagtcc gcccagattg atctgcccgc taataaactg cggcaataac
     6121 agaaagtagg gaagaacgct aagcgagcgc gaataaatat tttgccagta atcaagccac
     6181 cgctgacgat tcattaaacg atgccagttc tctttaatgg tatgaaaatt gtcgctcaac
     6241 tcctggcgtt gtagagactc agcattgctt agcgcaatca gttctgcctg tttattatgc
     6301 tgcacaagat tagttcgaaa tgtcgcttcg ctacgttgtt tttccacatt aagcggacga
     6361 atacgtttac ccaccttatg ggtaaataaa gttccaccga tcacaatgag cacgacggta
     6421 tagaccatat acccctggat attccattct gttccaccaa cagtgaatga gagcgtaccc
     6481 gcgctttgcc aaagaataac ggtaaaggtg atcagcatgc taagtgactg gatgaagcca
     6541 aatgaaagac tgagcgtttt gctgatcagt aagagaatgt cttcagcgat acgttggtca
     6601 gggttatctg tatttttatg ctcgccgtag atctgcgtga agtagtagtt tttatctgca
     6661 aaccaccgat taaggtagta atctgttaac cattcgcgcc agcgaatagt cagcagttta
     6721 attaaccatg ttttgtttac ggatattaat acaaagatcc ccagcagggc agggaaccaa
     6781 aggacaagct gccaaagctt atcggtttct ttctggctta gcgcattgaa aaaatcatta
     6841 ttccagtcgt ttaaccagac ctgaatttta accacggcaa ggatcattgc gaggataata
     6901 atgattaaca acactgaagt tttattattc ttacgcagcc aaaagggctt caacagacag
     6961 agatactttg ctatcaacat acgaagcgta atgggaatgg ttatcattag cgaaaattga
     7021 ttgtgcca
//
LOCUS       DAPHBJ010000219         7006 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.221, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000219 DAPHBJ010000000
VERSION     DAPHBJ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.221"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..151
                     /locus_tag="RHZ76_003996"
                     /pseudo
     CDS             1..151
                     /locus_tag="RHZ76_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(202..972)
                     /gene="yafV"
                     /locus_tag="RHZ76_003997"
     CDS             complement(202..972)
                     /gene="yafV"
                     /locus_tag="RHZ76_003997"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HDV2814535.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            1153..1599
                     /gene="ivy"
                     /locus_tag="RHZ76_003998"
     CDS             1153..1599
                     /gene="ivy"
                     /locus_tag="RHZ76_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HDV2814536.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(1642..4086)
                     /gene="fadE"
                     /locus_tag="RHZ76_003999"
     CDS             complement(1642..4086)
                     /gene="fadE"
                     /locus_tag="RHZ76_003999"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HDV2814537.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALVKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            4326..4904
                     /gene="lpcA"
                     /locus_tag="RHZ76_004000"
     CDS             4326..4904
                     /gene="lpcA"
                     /locus_tag="RHZ76_004000"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HDV2814538.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            5110..5877
                     /locus_tag="RHZ76_004001"
     CDS             5110..5877
                     /locus_tag="RHZ76_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HDV2814539.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(5848..6588)
                     /locus_tag="RHZ76_004002"
     CDS             complement(5848..6588)
                     /locus_tag="RHZ76_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HDV2814540.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(6744..>7006)
                     /gene="yafQ"
                     /locus_tag="RHZ76_004003"
     CDS             complement(6744..>7006)
                     /gene="yafQ"
                     /locus_tag="RHZ76_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HDV2814541.1"
                     /translation="EYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDH
                     PLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
BASE COUNT         1694 a         1887 c         1785 g         1640 t
ORIGIN      
        1 cagagttgta tcctgtatca gtcctgcaaa atttcacgag tgctttatta actggatgcg
       61 tgactgccat tcatcaaatg ataaagacgt catcgcgatt gatggaaaag cacttccaca
      121 ctcctatgat aaaagtggtc gcaggagagc gggctttcgt agtcttcccc gactctcccc
      181 agccctaaac acaaccccca ctcaccgcaa cctaaactca tccgcatcct gccatgccgg
      241 aaacttttct ctatattccc gcaatgccat catcgacagc tccgcatcaa tgcgcgttgc
      301 ctgatgcgcg tcggcagtag cgataatctc gccttgtgga ttaatcaccc ggctgtcacc
      361 gcgataatgg cagccgttgc catcgctgcc gacgcgattg catcccgcca catacgcctg
      421 attctcaatc gcgcgggccg tcagcaatgc ctgccagtgc agagagcgcg gagcaggcca
      481 gttggcgacg tacagggcga ggtcataatc gttgagattg cgcgaccaca caggaaaacg
      541 taagtcgtag cacaccagcg gcaaaatacg ccagccgcgc cattccacaa tcactcgcgc
      601 attgcccgct ttataatgta gatgctcatc tgccatgcgg aacagatgac gcttatcata
      661 aaaatgtacc gtgccgcccg gctcaaccag caaaaagcgg ttaaccgaac cagactccgt
      721 ttgtaatgca acactgcctg caatcagcgc attgcactgc tgcgccttag ctgtcatcca
      781 gttcactacg tcatcttgtg ctagcgacga agctgccgct tccatggcaa agccgctggt
      841 aaacatctcc ggtagaacga tcacatcgcg cccggtaata ccttccagtt gacgatcaaa
      901 atgacgcagg ttggcaggac catccatcca caccagtggt tgctgcaaaa gcgtaatctt
      961 caaaccaggc acggtgtaca actcctttat gcgaagggtt ttataacttt aacaccttat
     1021 caggcagttg ccttagcgca gaataaattg ataacaaatg ctgatattgg aaatatctga
     1081 tttgcaaatt atcgtgttat cgccaggctt taggaggtta ataacatggg caggataagc
     1141 tcgggaggaa tgatgtttaa ggcaataacg acagtcgccg ctctggtcat cgccaccagt
     1201 gcaatggcgc aggatgattt aaccattagc agccttgcaa agggcgaaac caccaaagct
     1261 gcatttaatc agatggtaca agggcataag ctgcctgcct gggtgatgaa aggcggtact
     1321 tatactcccg cacaaaccgt aacgttggga gatgagacgt atcaggtgat gagcgcgtgc
     1381 aaaccgcatg actgtggctc gcaacgtatc gctgtgatgt ggtccgagaa atctaatcag
     1441 atgacggggc tgttctcgac tattgatgag aaaacgtcgc aagagaaact cacctggctg
     1501 aatgtgaacg atgcgctttc gattgatggt aaaacggtgt tgttcgcggc gttgaccggc
     1561 agcctggaaa accatccgga tggctttaat tttaaataat tagcggataa agaaacggag
     1621 cctttcggct ccgttattca tttacgcggc ttcaactttc cgcactttct ccggcaactt
     1681 taccggcttc gtcgccagct cttccggatc aaagtcatca acgttaatac tgcgcagacg
     1741 gctttcttca gctttcacca gaatagcggc ttcatcttta tcaatcagcc ccttcaccag
     1801 cgcgttgtgc gccagttcat ccagacgggt aaacggcagg tttttaccca gctctttaca
     1861 gatccgctga tgaattgggt cggcggcaat cacatccacc agcgcctctt ccagcaagcc
     1921 aaccggatta tgctcgctcg gcgtcaggta ctgaccgcga ccaatgcggg aacgggtggc
     1981 gttcggcact tgtaaaatct tcgccacttt atgatccagc ttgtcagaag gtgccagata
     2041 atgacgtccg gtcgggaaga tcaccacatt cagcagcccg gcaaccacgc ggttcgggaa
     2101 gttttgcagt aaatcatcca tcgcctgttc agcctgatac agcgcatctt gtacgcccca
     2161 gtgcaccagc ggcaggtcgg cttcattacg gccttcgtcg tcataacgct tcagcacggc
     2221 agaggcgagg tagagctggc ttaaaatatc ccccagacgg gccgagatgc gctcgcgacg
     2281 tttcaggctg ccgcccagca cagccatcga gacatcagaa agcagggcga ggttggcgct
     2341 caggcggttc aggtgctgat agtagcgttt agtggcatcg ccggttggcg tgctgctggt
     2401 taaaccgcgc gtcaggccca gccagaagct gcgaactttg ttgctaccga cgtgaccgat
     2461 atgtttgaac aacagtttat cgaacgcgtt gacgtcattg ttcttcgccg cttccatctc
     2521 ttccagcacg tacggatggc aacgaatcgc tccttgtccg aagatcatca tgctgcgggt
     2581 cagaatgtta gccccttcaa cggtgatggc aatcggtgcg ccctggtaag cacgcgccag
     2641 gaagttgctt tgcccgagca taatgccttt accgccggta atatccatcg catcaataat
     2701 cgactgctgc ccgcggtggg tacagtgata cttaacgata gccgacagca cggcaggttt
     2761 ttcgccgagc ataatgccgt aggtaatcag cgatgccgca gcatccatca cgtaggcatt
     2821 accggcaata cgcgccagcg gctcttcaat cccttccatc ttaccaatag agattttgaa
     2881 ctgacggcga atgtgagcat acgcgccggt tgccagcgct accgatttca cgccgccggt
     2941 tgagttggaa ggcagggtga tgccgcggcc taccgagagg cactccacca gcatccgcca
     3001 gccttgcccg gccattttcg gcccgccgat gatgtaatcg atcggcacga agacatcttt
     3061 accgcgcgtc ggtccgttct ggaacggtac gttcagcggg aagtggcgac gaccaatttc
     3121 cacgcccggc gtggtggttg ggatcagcgc acaggtaatg cctaaatctt ctgcaccgcc
     3181 gagtaatttt tccgggtcgg agagtttaaa cgccagccca agcacggtcg caatcggtgc
     3241 cagcgtaatg tagcgtttgt tccaggtcag acgcatcccc agcacctgct ggccctgcca
     3301 ttcgcccatg cagacaatcc cggtgtccgg aatcgcgccc gcatcggaac ccgcttccgg
     3361 gctggtcagt gcaaagcagg ggatctcctg accacgcgcc agacgcggca gatagtgatc
     3421 tttctgctcg tcagtgccgt aatgttgcaa cagttcgccc gggcctaatg agtttggcac
     3481 gccgacggta atcgccagga tcccgctcac gccggagagt ttttgcagca cgcgagactg
     3541 ggcataagcc gagaactcca gcccgccgta ctcttttttg atgatcatcg cgaagaaacg
     3601 atgctcttta aggtacgccc acaactccgg cggcagatcc gccagctcat gggtgatctg
     3661 gaaatcattc gccatccggc aggcttcttc taccgggccg tcgagaaacg cttgctcttc
     3721 ggcggtcagg cgcggctgcg gatagttatg cagctttttc cagtccggct tgccctggaa
     3781 caagtcgccc tcccaccagg tggtgcccgc atcaatcgct tctttctcag tgcgcgacat
     3841 cggcggcatc accttacgga aaccgcgaaa taccggcgcg gaaatcatcg acttacgcat
     3901 aggcgcaaag ttaaatggca cgaggataat ggccagaggc accagtaccc acgccgacca
     3961 cagaccagca acgccgaggg cggctgtcca ggcgagcaaa atcagactgc tgataaataa
     4021 gctcacgcgg tgatagaaca acgcgccgag caggacaacc gtagcgagaa tactcaaaat
     4081 catcataacg aaaagcccct tacttgtagg aggtctgacc acttgtgatg atatggttgt
     4141 agtggatgta aaaacattta gcaatgtgtt tacaatataa ttacaacaaa gctcacattg
     4201 ttgctgtttt tatccgcact tcaggtcaaa aagtcctggt catagcacct gcccgtactt
     4261 ctcgcttttg gcggtatccg gtacactgca ttttgtctat tacatttatg ctgaaggata
     4321 tcctcatgta ccaggatctt attcgtaacg aactgaacga agcggcggaa acgctggcta
     4381 actttttaaa agatgacgcc aatattcacg ccattcagcg cgcggcggtc ctgttagcag
     4441 acagctttaa agccggtggc aaagtgcttt cctgcggcaa cggcggttcc cattgcgacg
     4501 ctatgcactt tgccgaagag ttgaccggtc gctaccgtga aaaccgtccg ggctacccgg
     4561 cgattgctat ttctgacgtt agtcatattt cctgcgtcgg taatgatttc ggtttcaatg
     4621 atattttctc ccgctacgtt gaagcggtag gtcgcgaagg cgatgtactg ctggggatct
     4681 ccacctccgg taactctgca aacgtgatca aagcgatcgc agcggcgcgt gagaagggga
     4741 tgaaagtgat caccctgacc ggtaaagacg gcggcaaaat ggctggcacg gcggatatcg
     4801 aaattcgcgt accgcacttt ggttatgccg accgcattca ggagattcac attaaagtga
     4861 tccatatcct gatccagttg attgaaaaag agatggttaa gtaagtctgg cgtaggccgg
     4921 ataagacgtt tacgccgcat ccggcatttg tgcgctgatg cctgatgcga cgctgacgcg
     4981 tcttatcatg cctacaaatc tgtacgcgaa ccgtaggccg aataatgcgt tcacgccgca
     5041 tccgacctga aaattcttaa atcaatcttc gccgggggcc atgcgctccc gctgttgtgg
     5101 aggttaccca tgtgcgaatt gctcgggatg agcgccaacg tccctaccga tatctgcttt
     5161 agtttcaccg ggcttgtaca gcgtggtggt ggaaccgggc cacataaaga tggctggggc
     5221 attacctttt acgaaggtaa aggctgtcgc acatttaaag atccacaacc cagctttaat
     5281 tcccccatcg ccaaacttgt ccaggactac ccgataaaat cctgttcggt ggtggctcat
     5341 attcgccagg ctaatcgggg cgaggtggcg ctggaaaata ctcacccgtt tacccgcgag
     5401 ttatgggggc gtaactggac ttatgcccat aacgggcaac tgacgggcta caaatcactg
     5461 gaaaccggca acttccgccc ggtcggtgaa accgacagcg aaaaagcctt ttgctggctc
     5521 ctgcataaat taacgcagcg ttacccgcgc acgccgggca acatggcggc agtgtttaaa
     5581 tatatcgcct cactggcgga tgaactgcgg gcgaagggcg ttttcaacat gctgctttcg
     5641 gacggtcgct atgtaatggc gtattgctcg actaatttac actggatcac ccgccgcgcg
     5701 ccgtttggcg tggcaacgtt gctggatcag gatgtggaaa tcgacttcag ctcgcagacc
     5761 acaccgaatg atgtggtcac ggtgattgcg acacagccgc tgacgggcaa tgaaacctgg
     5821 caaaaaatta tgccaggcga atggcgctta ttttgcctcg gggagcgtgt agtttgatgc
     5881 cagttgtggc tgcaccactt cgtggcttaa cggcttgctg accacgtaac ggccattgac
     5941 cacagaaacg gtcggtggct tacgggtttg ctcaaagtag tcgtagcccg gcttcagctg
     6001 ctcccagaag tccttaaagt tggaatattt atggcgcttc atattggcgt cggtcatgcg
     6061 gaatgggtaa atactcacct gcacgctcgg ctgaccaaac accagcgcac cagtaacgaa
     6121 ctggaagatc tcatcaatac cctgattggt catcgcgtag cagccgatgg aaacacaatc
     6181 gccgtggatc atcagatatt tcccttcata accatgcgca cggtcatagg cattggggaa
     6241 accaatatta atcgctttgt agtaacggct gtctggtttt aactgattac gctggacgct
     6301 ataaaaccct tccgggcttt tgaaatcgcc ctgacgctgt tttggcccta agccgccgga
     6361 atatttacag attttatagc tgtcgagcag ttgatattgc tcgcccattt tgacgtagag
     6421 atcgagcgta cgttcttcct tgaagatctg gatgtagaca ggggatccca tcaactgctg
     6481 cttatactct ttgctcactg gcgtggtcga gctactgctg cccagcaaac cggcaaacga
     6541 aacgcacggg atcaacaaca tcgcaagaat taatgcgatt ttacgcatac tgcttattcc
     6601 ttgataaaac ggttacacac gccaggacgg caaaatggat cccaaatcgg aatagtctgg
     6661 atttggaagg ctcacattat caccaaaaga gttttacgca agcctgtcgg cgcagggttt
     6721 acaaatttca tcggaagcgg gctttaccca aatagcgccg cgtgagttcc agttctctca
     6781 aatcgtaaaa gtttatcggt aagtttgtaa atcaggatcc agtccggttc gacatgagca
     6841 tcgcgataac ctttccatga accttgcagc gggtggtctt tataaacagc tggaagcggt
     6901 aaagtattat tgataagaag cgtcataaga tatttcaatt tattcatatc cttatgacgc
     6961 ttttgtgcaa gttttacatc ctttgaaaat tgtcccgagt attcaa
//
LOCUS       DAPHBJ010000220         7005 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.222, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000220 DAPHBJ010000000
VERSION     DAPHBJ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.222"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            512..1501
                     /gene="araF"
                     /locus_tag="RHZ76_004004"
     CDS             512..1501
                     /gene="araF"
                     /locus_tag="RHZ76_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HDV2814542.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1571..3085
                     /gene="araG"
                     /locus_tag="RHZ76_004005"
     CDS             1571..3085
                     /gene="araG"
                     /locus_tag="RHZ76_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HDV2814543.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3100..4086
                     /gene="araH"
                     /locus_tag="RHZ76_004006"
     CDS             3100..4086
                     /gene="araH"
                     /locus_tag="RHZ76_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HDV2814544.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4253..5053
                     /gene="otsB"
                     /locus_tag="RHZ76_004007"
     CDS             4253..5053
                     /gene="otsB"
                     /locus_tag="RHZ76_004007"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HDV2814545.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIIHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5028..6452
                     /gene="otsA"
                     /locus_tag="RHZ76_004008"
     CDS             5028..6452
                     /gene="otsA"
                     /locus_tag="RHZ76_004008"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HDV2814546.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6459..6887)
                     /gene="uspC"
                     /locus_tag="RHZ76_004009"
     CDS             complement(6459..6887)
                     /gene="uspC"
                     /locus_tag="RHZ76_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HDV2814547.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1773 a         1634 c         1822 g         1776 t
ORIGIN      
        1 gatgaagtga tgattttata aaacgttttt tcatttttgc gagatggctc tcattatacg
       61 tgttctgtta attaatgagc acagtgataa ttaatggcga aatgaatgac gtgcattttc
      121 cacatctttg agttgcggtt attacaccat ttcaaaaact caacgccagg taatgcggcc
      181 tattgactgg ttaaaaagaa gacatcccgc atgggtacca aagacaacaa ggatttccag
      241 gctaatctta tggattaatc tgctgtgcat tcgacaattt gtctgacaaa ttggctttcc
      301 cttatgtctt ttcccgctaa atttatgcac gttctcactg taattctgcg atgtgatatt
      361 gctctcctat ggagaattaa tttctcgcta aaactatgtc aacacggtca cttatctttt
      421 agttaaaagg taatgctttg ttttccgatt aatttaacga atgtcattcg tttttgccct
      481 acacaaaacg acactaaagc tggagagaac catgcacaaa tttactaaag ccctggcagc
      541 cattggtctg gcagccgtta tgtcacaatc cgctatggcg gagaacctga agctcggttt
      601 tctggtgaag caaccggaag agccgtggtt ccagaccgaa tggaagtttg ccgataaagc
      661 cgggaaggat ttagggtttg aggttattaa gattgccgtg ccggatggcg aaaaaacatt
      721 gaacgcgatc gacagcctgg ctgccagtgg cgcaaaaggt ttcgttattt gtactccgga
      781 ccccaaactc ggctctgcca tcgtcgcgaa agcgcgtggc tacgatatga aagtcattgc
      841 cgtggatgac cagtttgtta acgccaaagg taagccaatg gataccgttc cgctggtgat
      901 gatggcggcg actaaaattg gcgaacgtca gggccaggaa ctgtataaag agatgcagaa
      961 acgtggctgg gatgtcaaag aaagcgcggt gatggcgatt accgccaacg aactggatac
     1021 cgcccgccgc cgtactacgg gatctatgga tgcgctgaaa gcggccggat tcccggaaaa
     1081 acaaatttat caggtaccta ccaaatctaa cgacatcccg ggggcatttg acgctgccaa
     1141 ctcaatgctg gttcaacatc cggaagttaa acattggctg atcgtcggta tgaacgacag
     1201 caccgtgctg ggcggcgtac gcgcgacgga aggtcagggc tttaaagcgg ccgatatcat
     1261 cggcattggc attaacggtg tggatgcggt gagcgaactg tctaaagcac aggcaaccgg
     1321 cttctacggt tccctgctgc caagcccgga cgtacatggc tataaatcca gcgaaatgct
     1381 ttacaactgg gtagcaaaag acgttgaacc gccaaaattt accgaagtta ccgacgtggt
     1441 actgatcacg cgtgacaact ttaaagaaga actggagaaa aaaggtttag gcggtaagta
     1501 atttgccgga aaaattcccc tctgcatgat gcagaggggg tgtgaacgac cagtgattca
     1561 cggagacgtt atgcaacagt ctaccccgta tctctcattt cgcggcatcg gtaaaacgtt
     1621 tcccggcgtt aaggcgctga cggatattag ttttgactgc tatgccggtc aggttcatgc
     1681 gttgatgggt gaaaatggcg caggaaaatc aactctctta aaaatcctca gcggcaacta
     1741 tgcgccaacc acgggttctg tagtgattaa tgggcaggaa atgtcctttt ccgacacgac
     1801 cgcagcactt aacgcgggtg tggcgattat ttaccaggaa ctacatctcg tgccggaaat
     1861 gaccgtcgcg gaaaacatct atctcggcca gctgccgcat aaaggcggca ttgtgaatcg
     1921 ctcattgctg aattatgagg cgggtttaca acttaaacat cttggtatgg atattgaccc
     1981 ggacacgccg ctgaaatatc tctccattgg tcagtggcag atggttgaaa tcgccaaggc
     2041 gctggcgcgt aacgctaaaa ttatcgcctt tgatgagcca accagctccc tttctgcccg
     2101 cgaaatcgac aatcttttcc gcgttattcg tgaactgcga aaagaggggc gggtgatctt
     2161 atacgtttct caccgtatgg aagaaatatt tgccctcagc gatgccatca ctgtctttaa
     2221 agatggacgt tatgtcaaaa cctttaccga tatgcagcag gttgaccacg acgcactggt
     2281 gcaggcgatg gtcgggcgcg acattggcga tatctacggc tggcaaccgc gtagttatgg
     2341 cgaggagcgc ctgcgtcttg atgctgtgaa agcaccaggc gtgcgtacgc caataagtct
     2401 ggcggttcgc agtggtgaaa ttgttgggct gtttggtctg gtaggggcgg ggcgtagtga
     2461 attaatgaaa ggcatgtttg gcgggacgca aatcaccgcc ggtcaggttt atatcgacca
     2521 acagccgatc gatattcgta aaccgagcca cgccattgcc gcaggcatga tgctctgccc
     2581 ggaagatcgc aaagcggaag gcattattcc cgtgcactcc gttcgcgaca atatcaacat
     2641 cagtgccaga cgtaaacatg tgctcggcgg ttgtgtaatc aacaacggtt gggaagaaaa
     2701 caatgccgat caccacattc gttcgctcaa catcaaaacg ccgggcgcgg agcaactgat
     2761 catgaatctc tcaggcggaa atcagcaaaa agccattctg ggccgctggt tatcggaaga
     2821 gatgaaggtc attttgctgg atgaacctac gcgcggcatt gatgttggcg ctaagcacga
     2881 aatatataac gtaatttatg cgctggcggc gcagggcgtg gcggtgctgt ttgcctccag
     2941 cgacttacct gaagtcctcg gcgttgccga ccggattgtg gtgatgcggg aaggtgaaat
     3001 cgccggtgaa ttgttacacg agcaggcaga tgagcgtcag gcactgagcc ttgcgatgcc
     3061 taaagtcagc caggctgttg cctgagtaag gagagtatga tgtcttctgt ttctacatcg
     3121 gggtctggcg cacctaagtc gtcattcagc ttcgggcgta tctgggatca gtacggcatg
     3181 ctggtggtgt ttgcggtgct ctttatcgcc tgtgccattt ttgtaccaaa ttttgccacc
     3241 ttcattaata tgaaagggtt gggcctggca atttccatgt cggggatggt ggcttgtggc
     3301 atgttgttct gcctcgcttc cggtgacttt gacctttctg tcgcctccgt aattgcctgt
     3361 gcgggtgtca ccacggcggt ggttattaac ctgactgaaa gcctgtggat tggcgtggca
     3421 gcggggttgt tgctgggcgt tctctgtggc ctggtcaatg gctttgttat cgccaaactg
     3481 aaaataaatg ctctgatcac gacattggca acgatgcaga ttgttcgagg tctggcgtac
     3541 atcatttcag acggtaaagc ggtcggtatc gaagatgaaa gcttctttgc ccttggttac
     3601 gccaactggt tcggtctgcc tgcgccaatc tggctcaccg tcgcgtgtct gattatcttt
     3661 ggtttgctgc tgaataaaac cacctttggt cgtaacaccc tggcgattgg cgggaacgaa
     3721 gaggctgcgc gtctggcggg tgtaccggtt gttcgcacca aaattattat ctttgttctc
     3781 tctggcctgg tatctgcgat agccggaatt attctggctt cacgtatgac tagtgggcag
     3841 ccaatgacgt caattggtta tgagcttatt gttatctccg cctgcgtttt aggtggcgtt
     3901 tctctgaaag gtggcatcgg aaaaatctca tatgtggtgg cgggtatctt aattttaggc
     3961 accgtggaaa acgccatgaa cctgcttaat atttctcctt tcgcgcagta cgtggttcgc
     4021 ggcttaatcc tgctggcagc ggtgatcttc gaccgttaca agcaaaaagc gaaacgcact
     4081 gtctgatgct tttttccaca acaatttaac gttttttccc accacagcca gccgccacaa
     4141 cggttggctg ttcttcattg caaatggcga cccccgtcac actgtctata cttacatgtc
     4201 tgtaaagcgc gttctgcgca aaacaataag aaaagagaag gaggagagcc gggtgacaga
     4261 accgttaacc gaaacccctg aactatccgc gaaatatgcc tggttttttg atcttgatgg
     4321 aacgctggcg gaaatcaaac cgcatcccga tcaggtcgtc gtgcctgaca atattctgca
     4381 aggactacag ctactggcaa ccgcaagtga tggtgcattg gcattgatat cagggcgctc
     4441 aatggtggag cttgacgcac tggcaaaacc ttatcgcttc ccgttagcgg gcgtgcatgg
     4501 ggcggagcgc cgtgacatca atggtaaaac acatatcatt catctgccgg atgcgattgc
     4561 gcgtgatatt agcgtgcaac tgcatacagt catcgctcag tatcccggcg cggagctgga
     4621 ggcgaaaggg atggcttttg cgctgcatta tcgtcaggct ccgcagcatg aagacgcatt
     4681 aatgacatta gcgcaacgta ttactcagat ctggccacaa atggcgttac agcagggaaa
     4741 gtgtgttgtc gagatcaaac cgagaggtac cagtaaaggt gaggcaattg cagcttttat
     4801 gcaggaagct ccctttatcg ggcgaacgcc cgtatttctg ggcgatgatt taaccgatga
     4861 atctggcttc gcagtcgtta accgactggg cggaatgtca gtaaaaattg gcacaggtgc
     4921 aactcaggca tcatggcgac tggcgggtgt gccggatgtc tggagctggc ttgaaatgat
     4981 aaccaccgca ttacaacaaa aaagagaaaa taacaggagt gatgactatg agtcgtttag
     5041 tcgtagtatc taaccggatt gcaccaccag acgagcacgc cgccagtgcc ggtggccttg
     5101 ccgttggcat actgggggca ctgaaagccg caggcggact gtggtttggc tggagtggtg
     5161 aaacagggaa tgaggatcag ccgctaaaaa aggtgaaaaa aggtaacatt acgtgggcct
     5221 cttttaacct cagcgaacag gaccttgacg aatactacaa ccaattctcc aatgccgttc
     5281 tctggcccgc ttttcattat cggctcgatc tggtgcaatt tcagcgtcct gcctgggacg
     5341 gctatctacg cgtaaatgcg ttgctggcag ataaattact gccgctgttg caagacgatg
     5401 acattatctg gatccacgat tatcacctgt tgccatttgc gcatgaatta cgcaaacggg
     5461 gagtgaataa tcgcattggt ttctttctgc atattccttt cccgacaccg gaaatcttca
     5521 acgcgctgcc gacatatgac accttgcttg aacagctttg tgattatgat ttgctgggtt
     5581 tccagacaga aaacgatcgt ctggcgttcc tggattgtct ttctaacctg acccgcgtca
     5641 cgacacgtag cgcaaaaagc catacagcct ggggcaaagc atttcgaaca gaagtctacc
     5701 cgatcggcat tgaaccgaaa gaaatagcca aacaggctgc cgggccactg ccgccaaaac
     5761 tggcgcaact taaagcggaa ctgaaaaacg tacaaaatat cttttctgtc gaacggctgg
     5821 attattccaa aggtttgcca gagcgttttc tcgcctatga agcgttgctg gaaaaatatc
     5881 cgcagcatca tggtaaaatt cgttataccc agattgcacc aacgtcgcgt ggtgatgtgc
     5941 aagcctatca ggatattcgt catcagctcg aaaatgaagc tggacgaatt aatggtaaat
     6001 acgggcaatt aggctggacg ccgctttatt atttgaatca gcattttgac cgtaaattac
     6061 tgatgaaaat attccgctac tctgacgtgg gcttagtgac gccactgcgt gacgggatga
     6121 acctggtagc aaaagagtat gttgctgctc aggacccagc caacccgggc gttcttgttc
     6181 tttcgcaatt tgcgggagcg gcaaacgagt taacgtcggc gttaattgtt aacccctacg
     6241 atcgtgacga agttgcagct gcgctggatc gtgcattgac tatgtcgctg gcggaacgta
     6301 tttcccgtca tgcagaaatg ctggacgtta tcgtgaaaaa cgatattaac cactggcagg
     6361 agtgcttcat tagcgaccta aagcagatag ttccgcgaag cgcggaaagc cagcagcgcg
     6421 ataaagttgc tacctttcca aagcttgcgt aggagcggtt aatcacccgt aagtggaact
     6481 aacagcacat cgacctcact tgaggcaatc actcttttcg ccgagcagga tgctcgcgaa
     6541 aagaaactgt gattgtgatt accgcaaatc accaaatcga aatgatgttt gtgacatact
     6601 tccagaatat gttcgcttaa ctcaccgtag gcaataaacg tcttgtcaac gggatacccc
     6661 gcatcctgaa ttaacttatc aagaaagctt tgcgtttctt cttgcatcac gctgcgtaaa
     6721 tcttcaagca tcggcgcggc taattgattg tacatttccg ggtcggaagc gagagtaatt
     6781 aaactgatgt gtccctttac tggcctggcg atagataccg cttttgccag cagttgctga
     6841 ctttccggtg taaccgcaac agcgacaaga atattgctat agctcataac ctgttcctta
     6901 ttctgtgaac ttcaggtgac attaaagctt tactcctaat ggagaaacga cgcaatccca
     6961 actcggtcaa acaggtaaat attagtctga ttattagtaa agaca
//
LOCUS       DAPHBJ010000221         6807 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.223, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000221 DAPHBJ010000000
VERSION     DAPHBJ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.223"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..161
                     /locus_tag="RHZ76_004010"
     CDS             <1..161
                     /locus_tag="RHZ76_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HDV2814548.1"
                     /translation="ASMNPARSFGPALAMGYWDNQWLYWAAPLSGGLAAVACCQLFMP
                     QLKSPSPE"
     gene            complement(211..708)
                     /locus_tag="RHZ76_004011"
     CDS             complement(211..708)
                     /locus_tag="RHZ76_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004196778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV2814549.1"
                     /translation="MAEIIIRKMHEEDWCAVREIYQEGIATRNATFQTAAPEWLEWNE
                     GHLQDCRYVATVDNRVVGWAALSPFSRRHAYRGVAELSIYVSTHFQGKGAGRALLSGL
                     IKGSEGAGFWTLLAGIFPENQASVALHRSQGFREVGCREKVGEMNGKWRDVLILERRS
                     RTVGC"
     gene            1040..1366
                     /locus_tag="RHZ76_004012"
     CDS             1040..1366
                     /locus_tag="RHZ76_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2814550.1"
                     /translation="MELKIAAMVLKELGHTTRLDIYKTLVKAGRQGLPVGELQQHLAI
                     PASTLSHHLSSLISVSLVRQERQGRTLFCHACYDNLAALIAFLTEECCADEYAPPGFM
                     SPPETK"
     gene            1363..2080
                     /gene="arsH"
                     /locus_tag="RHZ76_004013"
                     /pseudo
     CDS             1363..2080
                     /gene="arsH"
                     /locus_tag="RHZ76_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151351.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical resistance protein ArsH"
     gene            2089..2634
                     /locus_tag="RHZ76_004014"
     CDS             2089..2634
                     /locus_tag="RHZ76_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDV2814551.1"
                     /translation="MSDVTAFNNCMSVFWQQHEAELSRFLASRTGDREQAADLLQEVF
                     LRARACADRFCEMENPRAWLYRTARNLLTDEYRAARDVVVLEDEIPLPDAFHDAVSTL
                     EICLPETLQALPDEEIWLIEEADLNRRPQRRLADELGITLTAFKSRLLRARKHLKETM
                     TELCQVEADDASSVCCHKKMD"
     gene            2710..3072
                     /locus_tag="RHZ76_004015"
     CDS             2710..3072
                     /locus_tag="RHZ76_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HDV2814552.1"
                     /translation="MSKIEIFEAAGCCATSSVVVSDEAVKWNSSAEWAKKHGVNIQLY
                     SLAKNPQQFLNTPVIRAFLNTSGMESLPATLLDGQLVMAGKLPSREDIARWAGISLTQ
                     DWNEDSTQPRCCSIPRMP"
     gene            3097..4848
                     /gene="arsA"
                     /locus_tag="RHZ76_004016"
                     /pseudo
     CDS             3097..4848
                     /gene="arsA"
                     /locus_tag="RHZ76_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_131199602.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical pump-driving ATPase"
     gene            4970..5398
                     /locus_tag="RHZ76_004017"
     CDS             4970..5398
                     /locus_tag="RHZ76_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV2814553.1"
                     /translation="MHIINAEEQHIPAIRRIYAHHVLHGTGSFETAPPNTQEMLARVK
                     NVQSRGFPWYIALQGGGKALLDHALTWARSQGYRQMIAVVGDCANVASVALHLRSGFT
                     EIGTLKDIGFKHGRWLDTVLLQCQLGKGSCTLPDSPVPGR"
     gene            complement(5431..5856)
                     /gene="arsC"
                     /locus_tag="RHZ76_004018"
     CDS             complement(5431..5856)
                     /gene="arsC"
                     /locus_tag="RHZ76_004018"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HDV2814554.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGNEPTVIHYLENPPSRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDQLIDFMLQHPILINRPIVVTPLG
                     RRLCRPSEVVLDILPDAQKGAFAKEDGEKVVDEAGKRLK"
     gene            complement(5869..>6807)
                     /gene="arsB"
                     /locus_tag="RHZ76_004019"
     CDS             complement(5869..>6807)
                     /gene="arsB"
                     /locus_tag="RHZ76_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HDV2814555.1"
                     /translation="LTPIVIAMLLALGFSKGTTLAFVMAAGFIADTSSLPLIVSNLVN
                     IVSADFFGLGFTEYASVMVPVDIAAIVATLVMLHLFFRKDIPPTYDLSLLKAPAKAIK
                     DLATFRTGWIVLILLLVGFFVLEPLGIPVSAIAAVGAVILFAVAKRGHAINTGKVLRG
                     APWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADKGLWAATLGTGFLTAFLSSIM
                     NNMPTVLVGALSIDGSTATGVIKEAMIYANVIGCDLGPKITPIGSLATLLWLHVLTQK
                     NMTITWGYYFRTGIVMTLPVLFVTLAALALRLSFTL"
BASE COUNT         1548 a         1893 c         1874 g         1492 t
ORIGIN      
        1 gggcaagcat gaacccggcc cgtagcttcg ggccggcatt ggccatggga tactgggata
       61 atcagtggct gtactgggcc gctccgctgt ctggcgggct tgcagctgtt gcgtgctgcc
      121 agctgtttat gccgcaactg aagtcaccct caccggaata aagtcccttc caggacagaa
      181 tgcctctgat gaacagaggc attctttttt tcagcaaccc actgtccggc tacgcctttc
      241 cagaatgaga acatctctcc attttccgtt catttcaccc actttttccc ggcagccgac
      301 ttccctgaat ccctggctgc gatgtaaagc aacactggcc tggttttccg gaaagatgcc
      361 agcaagtagc gtccagaaac ccgcaccttc cgagcccttt atcagcccgg agagcaaagc
      421 cctgcctgcg ccttttccct gaaaatgagt actgacataa atgctcagct ctgcgacacc
      481 gcggtatgca tgccggcggg aaaaaggaga tagcgcagcc catcccacca ctctgttatc
      541 aacagtggcg acgtagcggc agtcctgaag atgcccttca ttccattcca gccactcagg
      601 tgctgcggtc tgaaaggtgg cgttccttgt tgcaattcct tcctgataaa tttccctgac
      661 tgcacaccag tcctcttcgt gcatttttct aatgatgatc tctgccattt ctctgtaccg
      721 tatgtttgct gtattcatag gcagtcagaa aacgagcctg cctgggcttc ccggcggcgg
      781 gacataatgc gcccgtcagc aaaggatgaa ggtcatttca tttttagcgg gaatcggttt
      841 ctactgtgac ggattcatat gcatttttcc atatatattg tgacgtacct catttctgag
      901 aggtataaac accgatcatg ttttctccat gccgcgtaag tgtaaggccc cggagatgga
      961 gtcatattat ggacgtggtt gcgctcacta tatatttcta ttattctgga aatatagaag
     1021 taaaattacg aggaatggga tggagctgaa aatcgccgcg atggtgctga aagaacttgg
     1081 ccacaccact cgtctcgata tttacaaaac actcgttaag gccggacgtc agggactgcc
     1141 cgtgggggaa ctgcagcaac atctcgccat tccggcctcg accctgagtc atcatctttc
     1201 atcactgata tccgtctcac tggttcgcca ggaaaggcag gggagaacac tgttctgcca
     1261 cgcctgttat gacaaccttg ctgcattgat tgcctttctg acagaagagt gctgtgcaga
     1321 cgaatatgcc ccgcctggtt ttatgtcacc accggagaca aaatgattgc tgaactgaaa
     1381 aatgttgttc ctgagctgtt tgatacaggt ctcaccgggg tccgcctggg tgtaaagaca
     1441 gttgaccatc ctccccggat cctgatgttg tacggttccg ttcgagagcg gtcctacagc
     1501 cgtctggcca ccgaagaggc tgcccggctt cttaccgcca tgggtgcgga tgtccgcatc
     1561 tttaatcctt ccggtctccc tctgccggat gatgcagcgg atacgcaccc cagggttatg
     1621 gaacttcgcg aaatggtgcg ctggtctgaa gggatggtat ggtgctctcc tgaacgtcat
     1681 ggtgcgatga ccggcattat gaaagcccag attgactgga taccactatc agagagcgca
     1741 gtacgccctt ctcagggaaa aaccctggcc gtcatgcagg taggtctgcg gaggctccca
     1801 gtctttcaat gcggtaaacc agatgcgtat tcttggccgc tggatgcgaa tgatcactat
     1861 acctaatcaa tcctcggtag cgaaagcctg gcaggagttt gatgaagacg gacggatgaa
     1921 accatccccg tactacgacc gtattgtgga tgtaatggaa gagctgatga agtttaccct
     1981 gctgacccgg gagtatgcag cgtatctcgt cgatcgctac agtgaacgta aagagtcggc
     2041 agaagcactt tcccggcggg tgaatcagag caaaatctga gggctggtat gagtgatgta
     2101 acggcattca acaactgcat gagcgtgttc tggcaacagc atgaagcaga gctctcccgt
     2161 tttctggcat caaggacagg cgatcgggag caggcagccg atctgcttca ggaagtcttc
     2221 ctgcgcgccc gggcctgtgc agaccgcttc tgtgaaatgg agaacccgcg ggcctggctg
     2281 taccggacgg cgaggaatct gctgacggat gagtaccgtg ccgccagaga cgtcgtggtg
     2341 ctggaagacg agatcccgct gcctgacgcc ttccatgatg cggtttcaac gctggagatt
     2401 tgcctgcctg aaaccctgca ggccttacct gacgaggaaa tatggctgat agaagaggcc
     2461 gatctgaacc ggcgtcccca gcggcgcctg gccgacgagc tggggatcac gctgacggcg
     2521 tttaagtcgc gcctgcttcg cgcccggaaa catctgaagg agacgatgac ggaactctgc
     2581 caggtggaag cggatgatgc gtcttccgtc tgctgccata aaaaaatgga ttaaccgcgc
     2641 atctttttcc cgcctcactc gtttacctca gtgtaaagcc ccatcattca acactgaggt
     2701 aaacagtcca tgtcaaaaat tgagatcttt gaagccgcag gctgctgtgc gaccagcagc
     2761 gtcgtggtca gcgatgaagc cgtcaaatgg aactccagcg ccgaatgggc gaaaaagcat
     2821 ggtgtgaata ttcagcttta cagcctggca aagaacccgc agcagttcct gaatacgccc
     2881 gtcatcaggg ccttcctgaa cacgtcagga atggagtccc ttcccgctac cctgcttgac
     2941 ggccagctgg tgatggcggg caagctcccg tcccgggaag atatcgcccg ctgggccggt
     3001 atttccctca cgcaggactg gaatgaagac agcacgcagc cgcgctgctg cagcattccc
     3061 cgtatgcctt aatcccggag gagctcaaca atgacgatgc catttttaca gaatatcccc
     3121 ccgtttatct tcttcaccgg caaaggcggc gtggggaaaa cctctctggc ctgcgcgacg
     3181 gcggtatggc tggctgacca gggccggaga acgcttctgg tcagtaccga tccggcctcc
     3241 aatgtcggtc aggtcttcag ccagactgtg ggccaccgga tcactggtat ccgcaccgtg
     3301 gaaaaccttg cggcaatgga agttgacccg atggcggccg cgcaggctta ccgcgatcgc
     3361 gtgctcgatc ctgttcgtga actgatgcct gccgatgtca tcagcagcat tgaagaacag
     3421 ctttccggtt catgtaccac ggagatcgcg gcatttgatg aatttaccgc actgctgacc
     3481 agtcatgaac tgcgcgaaaa atatgatcat atcgtctttg atactgcacc aaccggacac
     3541 accatccgta tgcttgagct gccgggcgcc tgaagcgggt atctcgatgc acatcctgat
     3601 gctgcggcga acctcggccc tctggtaggg ctggagaaac aacaggacca gtacgctgac
     3661 gcagtgaaag ccctctctga tacggcactc acgcgactgg ttctggttgc ccgcgcccag
     3721 gcgtcgaccc tgaaagaggt ctcccatacc cacgaggagc tgtcggctat cggtctgcag
     3781 catcagcacc ttgccatcaa tggcgtgctg cctccctttg ccggtgaacg ggatccgctg
     3841 gcgcacagta ttctggcgcg ggaggaacgg gccttacggg ccatgcctga aaacctcgcg
     3901 catcttcccc ggtcgatgct gtatctgaag ccttttaatc tggttggtct ggacgccctg
     3961 agggcgctgt ttacagagag cacacctgtt ctgccggatc cgggagccac gctcacgacg
     4021 gtcgatttac cggagcttgc ctccctggtg gaggacctca gccgggcggg aaaagggctg
     4081 gtcatgacaa tgggaaaagg gggcgtggga aaaaccacgg tggcggctgc ggtcgcggtc
     4141 tcgctggcca gacgcggtca taaagtccac ctgaccacct ccgatccggc agccaacctg
     4201 tcttacaccc tggatggctc gctgccaggc cttcaggtta gccgtatcga tccaaaggca
     4261 gagactgagc gctatcgtcg ctttgtgctg gaaaatcagg gaaaagggct ggatgcggag
     4321 ggactggcgg tactggagga agatctccgt tccccctgca ccgaagagat tgccgtgttt
     4381 caggcattct cccggatcat caaagaggcc aacgaccatt ttgtcattat ggacactgcg
     4441 cccaccgggc atacgctgct gttgctggat gcaaccgggg cctatcaccg ggaaatggtg
     4501 cgccagatgg ggcatgctca cgatcacgtg ataacgccaa tgatgcagct gcaggatccg
     4561 gagaagacca gagtcattat cgtgacgctt gccgaaacca cgccggtact ggaagcggcc
     4621 gggctgcagc aggacttacg ccgggccggg attgacccct gggcgtgggt catcaataac
     4681 agcctcgcgg ccgcgaagcc gtcctccccg ttcctggtca cccgtgcccg tcgcgaactg
     4741 ccgctgatcg acgacgttgc cgggcattat gcacagcgca ttgccctgac gccactgctg
     4801 aaggacgacc cggtaggcgt ggacctgctg gctgaaatgg cgggctgaca ggtggggcga
     4861 cgccccacct ccacttaaaa catttcctta tacaggtcat aagatgaagc gcttaatctg
     4921 ccgtcgcgaa ttccggtagt gtgtcagtca acgtcacagg agtcatacaa tgcacattat
     4981 caatgctgaa gagcagcaca ttcccgccat acgccgtatt tacgcccatc atgtcctgca
     5041 cggcacaggc agcttcgaga cggccccccc gaacacgcag gaaatgcttg cccgggtgaa
     5101 aaacgttcag tcacgtggat ttccctggta tatcgccctg caggggggcg gaaaagcctt
     5161 actcgatcat gccttaacat gggcccggtc tcagggatac cgccagatga tagcggttgt
     5221 gggggactgt gcgaacgtcg cgtctgtagc gctgcatctt cgctccggat ttactgaaat
     5281 cggcacgctg aaggacatcg gtttcaagca tggccgctgg ctggacacgg tgttgctgca
     5341 gtgtcagctg ggaaaaggga gctgtacgct gccggacagt ccggtacccg gacgctgagg
     5401 caggagaaag ggggcatcgc cccctttcgg ttatttcagc cgtttccctg cctcatctac
     5461 tactttctcg ccgtcttcct tagcaaatgc gcctttttgc gcatccggaa gaatatccag
     5521 taccacttcg gaagggcggc acagacgcct tcccaacggt gtcaccacaa tcggacggtt
     5581 aatcaggatc ggatgctgaa gcataaaatc gattaactga tcgtcagtaa acttatcttc
     5641 cgcaagcccc agctcttcat aaggctcgac gtttttgcgc agcaaagccc ggactgaaat
     5701 gcccatatct gcaatgagtt tgaccagctc atcgcgtgac ggtggatttt caaggtaatg
     5761 aataacggtc ggttcattac cactgttgcg gatcatctcc agcgtattac gcgacgtgcc
     5821 gcaggccgga ttgtgataaa tggtgatgtt gctcatatca gtatctcatt acaaagtgaa
     5881 agagagacgt agcgccagcg ctgccagcgt tacaaacagc acaggcagag tcataacgat
     5941 cccggtgcgg aaatagtatc cccaggtgat ggtcatattc ttctgtgtaa ggacatgcag
     6001 ccagagcagc gttgccaggc taccaatagg tgtaatttta ggtcccagat cgcagccaat
     6061 cacgttggcg tagatcatcg cttctttgat aacgcctgtt gcggtgcttc catcgataga
     6121 aagagcgcca accagcacgg taggcatgtt gttcatgatg gaagacagga aggccgtcag
     6181 gaagccagta cccagcgtcg cagcccagag tcctttatcc gccagtacat tcagcacacc
     6241 tgaaaggtat tcggttagcc cggcgttgcg cagaccgtag accaccaggt acatccccaa
     6301 tgagaagatg acgatctgcc atggcgcgcc gcgcagcact ttgccagtgt taatggcatg
     6361 gcctcgttta gctactgcaa acaggatcac agcgccaaca gccgcaatcg cgctaacggg
     6421 aataccgagc ggctcaagga caaagaaccc aaccagcaga agtattaaaa ctatccagcc
     6481 ggttctgaag gttgccagat ctttaatcgc ttttgccggt gctttcagaa gagacaggtc
     6541 gtaagtcggt gggatatcct tgcggaagaa cagatgcagc ataaccagcg tggcaacaat
     6601 ggctgcaata tccaccggca ccattaccga cgcatactcg gtgaagccca gtccaaagaa
     6661 gtctgcagaa acgatgttaa ccaggttgga cacgataagc ggcaggctgg atgtatcggc
     6721 aataaaccct gcagccatga cgaatgccag tgtcgtgcct ttgctgaacc ctaatgccag
     6781 cagcatggcg atgactatcg gcgtcag
//
LOCUS       DAPHBJ010000222         6790 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.224, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000222 DAPHBJ010000000
VERSION     DAPHBJ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.224"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..109)
                     /locus_tag="RHZ76_004020"
     CDS             complement(<1..109)
                     /locus_tag="RHZ76_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDV2814556.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNI"
     gene            322..1113
                     /locus_tag="RHZ76_004021"
     CDS             322..1113
                     /locus_tag="RHZ76_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HDV2814557.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDLPDADSVE
                     KLHITQQECIAEGVEVICFPADVGDLSLHEEMLDAAQNLWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVTIDGGLSMPRF"
     gene            1123..1965
                     /locus_tag="RHZ76_004022"
     CDS             1123..1965
                     /locus_tag="RHZ76_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDV2814558.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIAHQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            1975..2751
                     /locus_tag="RHZ76_004023"
     CDS             1975..2751
                     /locus_tag="RHZ76_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDV2814559.1"
                     /translation="MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFNNQ"
     gene            2761..4302
                     /locus_tag="RHZ76_004024"
                     /pseudo
     CDS             2761..4302
                     /locus_tag="RHZ76_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
     gene            4299..6380
                     /locus_tag="RHZ76_004025"
     CDS             4299..6380
                     /locus_tag="RHZ76_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDV2814560.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPA
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDYAGFICHADKVRRATRDALLLNEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARIILDRDGNSIP
                     QTRNGFCDAWRFRSERTEDKKDKLLIHYCNGIFHVRETPVREFPIIRGIWFEKNWPGF
                     LNGTIYQPQDE"
     gene            6401..>6790
                     /locus_tag="RHZ76_004026"
     CDS             6401..>6790
                     /locus_tag="RHZ76_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDV2814561.1"
                     /translation="MRNIIRSINALLAALNITILAIIVACVTWQVAARFIFTSPSIFT
                     DELSRLLFICLGLFGGAYTAGQNRHLAIDLLPMMLKGKARRHLFLCIQIIVIIFATII
                     MVYGGGLLTMDTFDSGQTSPALGWQMGY"
BASE COUNT         1641 a         1671 c         1813 g         1665 t
ORIGIN      
        1 caatattatc gaccctggcc ccttcataac catattcggc aaattcatta accgcgcata
       61 acagcagctt ttccttcagg ctctgtacgt tgctggtatc aagatacata acgcttcccc
      121 acgacctctt caagataact atcttaaccc gattaattag aagctccagt aaaaaggata
      181 aaaacttgta ctttcatcaa gttatcaaat gtcctgtgat cactatcgca tatcaaccaa
      241 ccagatggtt gatctttctt gcggcacgtc gcaagctaat tgtcacttag aaaacaatat
      301 tcaaaaaaag agagctattg tatgaaatcc actcgtcctg ttgccgtgat taccggagcc
      361 gctcgtggta ttggaaaggg ctgtgcgctg gagcttgctc gtggcggatt taacctgctg
      421 attaacgatc ttcccgatgc cgacagcgta gaaaaactgc atatcacaca acaagaatgt
      481 atagctgaag gcgtggaagt gatttgcttc cctgccgatg tcggcgatct ctccttgcat
      541 gaagagatgc tcgacgcggc gcaaaacctg tggggacgtc tggactgcct gctcaacaac
      601 gcaggcattt cagtgaaaaa acgcggtgac ctcctcgacc tcgaaccaga cagcttcgat
      661 caaaacattg ccattaacac ccgcgcgccg ttcttccttg ctcaggcatt cagtaagcgt
      721 ttactggcac agccgaaacc ggaagcggaa ctgccgcacc gctcgattat tttcgtcagt
      781 tccatcaacg ccatcatgct ggcgatgaat cgcggcgaat acaccattgc caaaaccgcc
      841 gtttctgccg ctgctcgtct atttgcagct cgcctttgta atgaacaaat tggcgtttat
      901 gaagtgcgcc caggtctcat caaaactgat atgactattc cggcaacggc ttattacgac
      961 gaactgattg ccaaaggtct ggtgccgtgg ggacgttggg gctatccggc tgatattgct
     1021 tccactgtcc gcgcgatggc ggaaggtaaa ctgatttaca cctgtggtca ggcggtcacc
     1081 atcgacggcg gcttaagtat gccgcgcttc tgaggctgaa tcatgagaga tctgttacaa
     1141 cgcccggatt tattcagtat caataccgca acactgggct ataaaacgcc gctacccgcc
     1201 attattgatg cctgcgccgc gcgtggtatt ggcgcgattg ctccctggcg cagggagttg
     1261 caaggtgaag atctccagca aatcgcccac cagcttgccg ccagtaacat gagcgtttcc
     1321 ggtttgtgcc gtagcactta ctacaccgcg ccgacactgg cggaacgcaa actggcgatt
     1381 gatgataacc gccgggcgct ggacgacgca gcggtgctta acgccgcctg ttatatgcag
     1441 gtggttggcg gtctgccaac gggcactaaa gatctatatg aagcgcgaga acaagtgaaa
     1501 caagggattc gccagttgct gccacattcg aaagatgtcg gcgtgccaat tgcgctggag
     1561 ccactgcacc cgatgacagc tgccgatcgc tcttgcctgt gcacgttacg ccaggcgctg
     1621 gactggtgtg atgaactcga tcccgatggt gagtttggcc tcggcgtggc ggtggatgtt
     1681 tatcacgtct ggtgggatcc ggatctcgcc agtcagatcc tgcgtgccgg aaaacgcatc
     1741 ctcgcgtttc atgtttccga ctggttagtg ccgaccactg atctggtcaa tgaccgtggt
     1801 atgccgggag atggcgtcat caatattccg tcaattcgtc ggctggttga aaacgccgga
     1861 tttaatggcg ctattgaact ggaaatattc tcgccgtact ggtggcaaaa agatattaac
     1921 agcacgctgg atattagcgt cgatcgcatc gcgcattatt gttaaggaat gatcatgagt
     1981 gaccaacccg ttctgttcag cagagcggcg gcttcctgcc gcctgacatt gaaccgtgaa
     2041 gataaatgtc atgctattaa cgaagagatg atcgaatctc tcgaccatta tttaaatgaa
     2101 attgaaaacg acaccacatt acgattagtc gaattaacgg caaccggcga taaattcttt
     2161 tgtgcgggcg gcgatattaa atcgtggtcg gcctattcgc cattagatat gggcagaaaa
     2221 tggattaaac gcggtaatga cgtttttaat cgcctgcgaa acttacccca attaaccgtt
     2281 gcgaatctca acggtcatac catcggcggt ggtattgaac tggccttgtg ctgcgatatt
     2341 cgtattgctc gccccggcgc gaaattctct aacccggaag tgatgctcgg catggtgcct
     2401 ggctggatgg gcattgaacg tgtgcttaat caggttggtc cggtggtcgg tcgccagatg
     2461 ctgatgctgg gtaaacgcct gacggcgcag gaagctcagg cggcgaattt aattgatgaa
     2521 gtggtagaaa aagaacaggt ggaaagctgg atggcgaacc agttagcgca gctggaaaaa
     2581 tgtggtccgg tggcgcttgc acatattaag caactgattc tggcactgga aaataaacat
     2641 gccgattacc cgcaccagct actggccgga ttaatgtccg ccactcagga ttgccagcag
     2701 gcgacgcgcg cctttgccga aaaaagtagc gtgagcttca ataatcaata aggaaattgt
     2761 atgcgtaaga taacaaccgc cgaggcactg gcggcgcaaa ttcaggacgg tgcgactatt
     2821 gctattagcg gtaacggcgg cggtatggtg gaagccgacc atattctggc tgctattgaa
     2881 gcgcgtttcc tgcaaaccgg acaccctcgc gacctgacgc taatccactc gctgggtatt
     2941 ggcgatcgcg actgcaaagg cactaatcgt tttgcgcacg cagagatgct caaacgcatt
     3001 attgccgggc actttacctg atcgcccaaa atgcaggcgc tggtaaaaaa taacacgatt
     3061 gaagcctact gctttcctgg tggcgtgatt caggcactgc tacgggagat cggcgccgga
     3121 cgtccggggc tttttaccca cgttgggctg ggatcgtttg ttgatccacg caatggcggc
     3181 ggtaagtcga atgaatgcac taccgactag ctggtagaac tgattgaaat cgacggtgaa
     3241 accaaacttc gttatcgccc tttcaaagtg gattacgcga ttttgcgtgg cacttatgcc
     3301 gatcctcgag gcaacgtcag ccttgaagaa gaagcgattg atatggatag ctattccatg
     3361 gcgctggcag cacacaacag cggcggcaaa gtgttcgtac aggtacgcga tgtgctggaa
     3421 gctggcgcga ttgaaccacg ccgggttaaa ttaccgggca ttctggttga tggcatcgtt
     3481 gaacaccgcg aacagccaca aacctatctt ggcggttacg acctgaccat cagcggccaa
     3541 catcgccgtc tgagttctaa cgacgctatt gaactggtta gtcatccggt acgtcgcctg
     3601 attgcccgtc gggcagcacg cgaactggta gctggcgctt caaccaactt tggctttggt
     3661 attcccggcg gcattccagg agtagcgctg cgcgaaggcg ttccttacca aagtttatgg
     3721 ctgagcgtcg agcaaggcgt acataacggc atgatgctgg atgatgcgtt tttcggctgc
     3781 gcccgcaacg ccgatgccat tattccatca ctcgatcaat ttgagtgcta cagtggcgga
     3841 gggatcgata tcaccttcct cggcatggga gagatggatc agtacggtaa cgtcaacgtc
     3901 tcccatctca atggcaatct gattggcccc ggcggatttc tcgaaattgc gcaaaatgcc
     3961 cgtaaagtgg tgttctgcgg cacgttcgac gctaaaggca gcaagatcga tgtaacgcca
     4021 gatggcctgc atatcgccca gtcaggtcaa atccctaaac tggttaccca ggtggaaaaa
     4081 atcactttta gcgccgccta cgcacagcaa agtggtcagg aagtgttgta tatcactgaa
     4141 cgcgcagtat tccagttaac ggcagaaggc gttgaattaa ttgaaatcgc gccgggtgtg
     4201 gagattgagc gcgacattct gccgtatatg gctttccgtc caattatcaa gcacccacgc
     4261 ctgatggaaa gtagcctgtt tacgccgatg gaggatgcat gagtcagctt gatgacacca
     4321 tccttgatgc gctgacgcac gttactttcc caaagggttt tgcacaggca gagcccgcat
     4381 gggttgtcac ggtagacggt gttgattacc cactctggca aacagatgcc ctggtagtcg
     4441 gcagtggtgc agcaggatta cgtgcggcag ttgaacttaa acgccgccag caaaatgtgc
     4501 tgatcgctac cgccgggtta tatatgggga cgtcggcatg ttctggttcc gataaacaga
     4561 cactatttac cgccgctacc gcgggcaacg gcgacaactt caccaaactg gcagaggcac
     4621 tggcgagcgg tggggcgatg gatcacgaca ccgcttatgt cgaagcggta ggttctctgc
     4681 acactctagg cgggttgcaa tatcttggtc tggaattacc ggaagatcgc tatggcgcga
     4741 ttcttcgtta tcaaaccgac catgacgaag ccgggcgtgc aacctcgtgt gggccgcgta
     4801 cctcaaggtt aatggtgaaa gtgctgttgg aagaagtaca gcgcctcgcc attccagtgt
     4861 tgaccagtgc aacagtgatt aaactgctgc atcagcgtga cgaaaacggc gaagaccgcg
     4921 tggcaggggc aatcctcgcg accggtcatc gcgcccataa cccatggggg ctggcaattg
     4981 tgactgcgcc caatgtggta ctggcaacgg gcggaccggg cgagctttat cgcgacagtg
     5041 tttatccaca taaatgcttc ggctcgctgg ggctggcgct ggaggaaggt ctgacgctaa
     5101 ccaatctgac cgaaagccag tttggtatag gcacgccgcg tagcacgttt ccgtggaatt
     5161 tatccggcac ctatgtacag gtgatcccgt atatctattc cgtggatgct gagggtaacg
     5221 agtataactt cctcgcggat tactatcgca ccacccagga gctggcttca aacattttcc
     5281 gtaaaggcta tcagtggccg ttccacgcca ctcgggtgat ggattttggc tccagcttgt
     5341 tagatatggc agtagcgcaa gagcagcaat cagggcgtca ggtatttatg gatttcaatc
     5401 gcaatcctga agcggtgccg ggtgacctgc cgttctcatt agatcgactg gacgacgacg
     5461 ttcgcgcgta tctggaaaat aacgacgctc tggcaccatc gcccatcgaa cggctgcaac
     5521 gaatgaatcc gctgtctatc tcgctgtata agatgcacgg ttacgatctc accacacagc
     5581 cattgcagtt tgccatgaat aatcagcata tgaatggcgg cattgaagtg gatatctggg
     5641 gacaaacctc cctgcccggc tgtttcgccg tgggggaagt tgctggcaca cacggcgtca
     5701 ctcgccctgg tggtgcggca ttgaatgccg ggcaggtttt tgctgttcgt ctggcacgtt
     5761 ttattggttg cacgcaaaaa cgtaatattg atggagatat agcacagctg gtagctccgg
     5821 cactggcttc tataagagag atcattactc aggcgcacga taacgggacc gggatgccgt
     5881 tgtcggttgt gagagaaaaa attcaggcac gaatgtctga ctatgcggga tttatttgcc
     5941 atgccgataa agtccgacgt gccactcgtg atgccctgct attgaacgaa tttgtccaac
     6001 ggcatggatt ggctatcaaa catgtgggtg aagttgccga gctgtttatg tggcggcata
     6061 tggcactgac ttctgccgcc gtattaacgc aattaacgca ttatattgac gcaggtggtg
     6121 gtagccgggg agcacgtatt attctcgaca gggatggaaa ttctattccg caaacacgaa
     6181 atggtttttg tgatgcctgg cgctttcgtt cagagcgtac tgaggataaa aaagataaac
     6241 tacttattca ttattgcaac ggtatctttc acgtcagaga aacacctgtt cgtgagtttc
     6301 ctattatccg aggtatttgg tttgaaaaaa actggcctgg ttttcttaat gggacaattt
     6361 accagccaca ggatgaatga tattgcaaag gttaatcact atgcgaaata taattagatc
     6421 tattaatgct ctacttgcag cattgaacat taccattttg gcgatcatcg tggcatgtgt
     6481 gacgtggcag gtggctgcac gatttatatt tactagtccc agtattttta cagatgaact
     6541 cagccgtttg ctctttattt gtcttggact ttttggtggg gcatacaccg cagggcaaaa
     6601 ccggcatctg gcgattgacc tgctacctat gatgctgaaa ggaaaagccc gacgtcattt
     6661 atttttgtgt attcagatta tcgttattat cttcgctacc atcattatgg tatatggtgg
     6721 tggtctatta acgatggata cattcgattc aggtcagacc tcgcccgctt taggctggca
     6781 gatgggatat
//
LOCUS       DAPHBJ010000223         6699 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.225, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000223 DAPHBJ010000000
VERSION     DAPHBJ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.225"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(89..574)
                     /gene="sixA"
                     /locus_tag="RHZ76_004027"
     CDS             complement(89..574)
                     /gene="sixA"
                     /locus_tag="RHZ76_004027"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HDV2814562.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(777..2921)
                     /gene="fadJ"
                     /locus_tag="RHZ76_004028"
     CDS             complement(777..2921)
                     /gene="fadJ"
                     /locus_tag="RHZ76_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HDV2814563.1"
                     /translation="MEMASAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELVKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMSKRGESFWKTTATDLQ"
     gene            complement(2921..4231)
                     /gene="fadI"
                     /locus_tag="RHZ76_004029"
     CDS             complement(2921..4231)
                     /gene="fadI"
                     /locus_tag="RHZ76_004029"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HDV2814564.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDFNKARIMSQRLKLF
                     SRLRLHDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLNELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4412..4696)
                     /locus_tag="RHZ76_004030"
     CDS             complement(4412..4696)
                     /locus_tag="RHZ76_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDV2814565.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIGFTFACEAEMLIFQLGLR"
     gene            5068..6408
                     /gene="fadL"
                     /locus_tag="RHZ76_004031"
     CDS             5068..6408
                     /gene="fadL"
                     /locus_tag="RHZ76_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HDV2814566.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTQQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1663 a         1817 c         1598 g         1621 t
ORIGIN      
        1 tcgatagtag tgaggttgac agatatccgc tgcctgatga gcaattatgc ccaatgaatc
       61 tacctcattg ggcataattt ggaactcatc agatagcttt tgccatcttc agattacacg
      121 ggctcatctg ccagttgaat gtgccgtttc cgctttcatc aagcgttacg ctggcaatgg
      181 ccgaggtggt aaacataggc ggcgtttcgc ccggacataa ctcggcaacc agatacccga
      241 ctaacggtaa gtgagagatc accagtaccg aagcaacacc ttcattggtc aacgcctgca
      301 aataggcact gaccagacca acatcgccgc agggcgttaa ctccggcaga acttctgcac
      361 tggaaggcag gttcagacaa tcccctactt cttccagtgt ttgctcggct cgcaggaacg
      421 ggctcaccag aacgcgttcg atttccactt tttgaccttt tagccagttc gccatcaggc
      481 gagattcgtc acaaccatta gtggtcagag gacgaacgga atcactggcg gcatcgaggg
      541 ctgcgtcgcc gtgacgcatg ataaaaactt gcatattgca ccgcttttgt taaccagatt
      601 cgcccgtttt ctttcatctg aaaccagaga aagaaaacgg tggccggcat tgtgccttat
      661 ccattcaccg aatgaaacgc tgttttttac ctcaatggcg taagtatagt caatatgcgt
      721 ttacattttg agcaacactc cgccattcag cgcggattca tatagctttg accttcttat
      781 tgcaggtcag ttgcagttgt tttccaaaaa ctttccccac gcttgctcat ctcgactaaa
      841 cgctcgcaag gggtaaaacg ggaaccatac tgcgtggcca gtcgttgcat tattgcaacc
      901 acttcgcccg cgccgagaga atcgatatag cggaacggtc caccgagaaa tggcggaaaa
      961 ccaatgccaa ataccgcacc aatatcccca tcacgcacgc tacggataac ctgctcatca
     1021 acacaacgta ctgcttcatt cagcatcaac atcacacacc gttcagcaac ctgcggtgcg
     1081 gagagtcgcc cctgcccttg tgcgccaatg agcgggtaaa tggcaggatc gacctgtttt
     1141 ttgcttttac gccctttctg accataaaga tagaaacccc gaccattttt tctgcctttg
     1201 cgatcgtcgt tcaaaattga agaaacaaca tttgcaggcg cgctaaaacg ttctccataa
     1261 gcggcttcca gtacaggaat aattttagtc ccggtgtcga ttcctacctc atccaaaagt
     1321 tggattgggc ctaccggaaa accaaatttc actagcgcgg catcaatgtg ctctacccgt
     1381 tcaccttcgg tcagcatgcg gatagcttca ttaatgtaag gcgctaagat gcgattgacg
     1441 taaaacccgg ctttgtcacg cacgacaatg ggcgttttgc cctgtttttt cgccagtttt
     1501 accgtagtgg cgatggtttg cgctgatgta ccagcatgag gaataatctc caccagcggc
     1561 attttttcca ccggactgaa gaaatgcagg ccgataactt gctcaggtcg cgtggcgtga
     1621 gcggcgatat caccaatcgg caaagatgac gtattcgaag caaagatggt atgagcggcg
     1681 caattttgct ccacttccgc caccatctgt tgttttaatt cgagattttc aaacaccgct
     1741 tcaataatca gatcgcgatg ggcaaagccg cgatagtccg tcgttccgga gattaatgcc
     1801 agctgtttgt cacgttcgct ggctttgaga tgacgacggc gaactttgcc ctccagctga
     1861 tcccaactgt acttcagcgc atgatttatg ccctgcgggt tgatatcttt aattctgacc
     1921 ggaagccccg ctttacaagc agtgacataa gcaataccgc cgcccatcaa gccaccacct
     1981 aaaatcccca cgctgtttaa tggcgcaggc ggcgcatcac tgccgggatc tttcttcacg
     2041 tccgtactgg caaaaaagat actacgcaga gcctgcgatt gcggcgtcat cgccagttcg
     2101 ccaaacgccc gtgcttcagc gtcatagcca ctgctggtgc cctgcgctaa tcccgtttcg
     2161 acaacctcca ggatgcgttc tgtcgccgga tagttacctt gagttttgtg ttctgttttc
     2221 ttgccgacca ttttgaacag caacgcacga cctaacggcc ccgccagaat acgctcgcgt
     2281 acaggtagag ggcgggaaga tggacgatcc tgctttacca gctcaacagc ggcttccagc
     2341 aaaatggagt gcggaacgac gtcatccacc agccccagct ttaacgcctg tttcgcccga
     2401 agttgttttc cggtgaggat catctctaat gctgtgctga cgcctatgag acgcggtaaa
     2461 cgctgggtgc cgcctgaacc gggtaacaat ccaagttgta cttcaggcaa accgagcacc
     2521 gttttaggat cgtcagtaca aacgcgaccg tggcacgcca gcgccagctc cagcccaccg
     2581 cccaggcaag cgccatgaat agcggcaata accggaatgg gcaaagcatg aatctccgcc
     2641 atcaactgtt ggccctgccg cgccagagct tccgcttctt gcgccgtttt gcagttgccg
     2701 atcatgttga tgtctgctcc ggcgataaag ttgtccggtt tagcggagac aaacaccacg
     2761 ccgcgcaact ctttgttttc acggagttgc ttaataatgg cgcgcacctg cgaggcaaat
     2821 tccgccttca gggtattcat tttctcacca ggaacatcaa tggtgataac ggcaatgttg
     2881 tccagacgaa cattaagagt aaacgctgat gccatttcca ttattccgcc tccagaacca
     2941 ttgccgcgcc aagcccaccg gcagcacagg cggtaactaa accaaatcca ccgccgcgac
     3001 ggcgaagttc attcagtgtc tgggtaatca tccgcgcgcc ggtcgccgcg aagggatgcc
     3061 cataagcaat cgaaccgcca agcacgttaa atttgctatc gtccacttcg ccagtggcat
     3121 gtgcacgccc cagtacttca cgagcaaaac gttcactacc cagcaactga atattcgcca
     3181 gtgtctgagc tgcaaaggct tcgtgcatat cgatcaatgt cagatcgctc atcgtcaaac
     3241 cggcacgctc cagcgccagc ggtgttgacc aggctggacc gagcaacatg tcctgccaga
     3301 catcaatcgc agtaaatgcg tagctgcgca gataccccag cggcaccagc cctaattctt
     3361 tcgcccggga ttcagtcatc aggatcaccg ctgccgcgcc atcggtcagc ggcgtactgt
     3421 ttgccgccgt taccgttccg tgtttgcgat caaacgccgg gcgcagcttt gcgtaatcgg
     3481 caagcgagga attaccgcga atattgttgt cttcgacaag cggttgttta taaggaggga
     3541 taaaggcagt catcacctct tctttgagtt ttccgtctga ccatgcctga gcggcacgct
     3601 gatgcgaacg gtgcgctaat gcatcttgct gttctcgggt gatgccgtag gttttcgcca
     3661 tttgctctgc ggtgtcaccc atccgcaagc cggtagaata ttctgctacc gcaggcggta
     3721 cgggcattaa gtcgtgcaaa cgcaggcgag agaagagttt caggcgctgg ctcatgattc
     3781 gagctttgtt gaaatcaacc agcacgcgcg ccagtttttt actgacgcca attggcaata
     3841 cggaagagga atctgcccca ccggcaatcc ccgctcgaat agttcccgcc atcaggcttt
     3901 ctgcgacgtt tgcaactgcc tggaaactgg tagcgcaagc gcggctgacg ctgtaagcat
     3961 cggtatgcac attcattccc gtaccgagaa caatttcacg cgcaatgttg ggggcttcag
     4021 gcatttgtac gacctgacca aagaccagtt gttcaatcac ttcagcgggg atctcgctgc
     4081 gtgccagcag ttcgcctacc accatcttcc ctaaatcaac cgcgggaatg ccatgaaaag
     4141 ccgtcgcctg gcgggcaaaa ggcgtacgta aaccgctaac aatggcgata cgatcgccct
     4201 ggcgggtaac cagcggtaaa acctgaccca taacactccc ctgtaaaaaa ataaataaag
     4261 tggtctgacc tgatcatagt cttaaccatt tttttacatt tagccaagcg gagaaaaggg
     4321 aaagtgggag ctatgacaca gagagaaaag aagaagagaa aagaaaacgc ccctgccagc
     4381 aactgacagg ggcgtacgta cccgagagga attaacgcag acccagctgg aagatcagca
     4441 tttcagcttc gcaggcaaaa gtgaaaccga tatccaggcg cacaccgtca gactcttcag
     4501 tgaaagtcgg ggagattttg cacggttcgg attccacgct acgggctttc tcagtcagcg
     4561 ccgccagcgt ttgttctgct tctgcgcggt ttgcaaacac gcggctgtaa gacgcggtgc
     4621 agtcggagtt gtccataatg gtgccaacat ccatacagca gcaaaccggg gtttcatcag
     4681 cactacattt actcatcgtt gatttcctct gtatgtgcac ccaaggtgcc agataaacgt
     4741 tgtggatatt ttacgcttcc ggaaagtgct gctccagttg ttaattctgc aaaatcggat
     4801 aagtgaccga aatcacactt aaaaatgatc taaaacaaaa ttcacccgaa tccatgagtg
     4861 cgccacctcc aaattttgcc agctggatcg cgtttcttag atcatatttg aaaaaagata
     4921 gaaacatact tgcaacattc cagctggtcc gacctatact ctcgccactg gtctgatttc
     4981 taagatgtac ctcagaccct acacttcgcg ctcctgttac agcacgtaac atagtttgta
     5041 taaaaataaa tcattgaggt tatggtcatg agccagaaaa ccctgtttac aaagtctgct
     5101 ctcgcagtcg cagtggcact tatctccacc caggcctggt cggcaggctt tcagttaaac
     5161 gaattttctt cctctggcct gggccgggct tattcagggg aaggcgcaat tgccgatgat
     5221 gcaggtaacg ttagccgtaa ccccgcattg attaccatgt ttgaccgccc gacattttct
     5281 gcgggtgcgg tttatattga tccggatgta aatatcagcg gaacgtctcc atctggtcgt
     5341 agcctgaaag ccgataacat cgcgcctacg gcatgggttc cgaacatgca ctttgttgca
     5401 ccgattaacg accaatttgg ttggggcgct tctattacct ctaactatgg cctggcaaca
     5461 gagtttaacg atacttatgc aggcggctct gtcgggggta caaccgacct tgaaaccatg
     5521 aacctgaact taagcggtgc gtatcgctta aataatgcat ggagctttgg tcttggtttc
     5581 aacgccgtct acgctcgcgc gaaaattgaa cgtttcgcag gcgatctggg gcagctggtt
     5641 gccggtcaga ttatgcaatc tcctgctggc caaactcagc aagggcaagc attggcagct
     5701 accgccaacg gtattgacag taataccaaa atcgctcatc tgaacggtaa ccagtggggc
     5761 tttggctgga acgccggaat cctgtatgaa ctggataaaa ataaccgcta tgcactgacc
     5821 taccgttctg aagtgaaaat tgacttcaaa ggtaactaca gcagcgatct taatcgcgcg
     5881 tttaataact acggtttgcc aattcctacc gcgacaggtg gcgcaacgca atcgggttat
     5941 ctgacgctga acctgcctga aatgtgggaa gtgtcgggtt ataaccgtgt tgatccgcag
     6001 tgggcgattc actatagcct ggcttacacc agctggagtc agttccagca gctgaaagcg
     6061 acctcaacga gtggcgacac gctgttccag aaacatgaag gctttaaaga tgcttaccgc
     6121 atcgcgttgg gtaccactta ttactacgat gataactgga ccttccgtac aggtatcgcc
     6181 tttgatgaca gcccagttcc ggctcagaat cgttctatct ccattccgga ccaggaccgt
     6241 ttctggctga gtgcaggtac gacttacgca tttaataaag atgcttcagt cgacgttggt
     6301 gtttcttata tgcacggtca gagcgtgaaa attaacgaag gcccatacca gttcgagtct
     6361 gaaggtaaag cctggctgtt cggtactaac tttaactacg cgttctgata acacgttcgc
     6421 ctggataaag tcacctgcat agcaggtgac tttaactccc ccacttcacc gaaagtagtg
     6481 tcctcattgc ttacctcctg agttttgcaa acagcctgat ggcagcttgc cctttcaaat
     6541 caataagcgg tatccacaga caaacttgaa gggaaaggca tattttcagg cgttctgctc
     6601 gtccttctca aagagtttac ttttctgaat ttccaggata ctcacccccc cctggctatt
     6661 gtgcgctcat acactcaaat taaagatagg ttctaaata
//
LOCUS       DAPHBJ010000224         6538 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.226, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000224 DAPHBJ010000000
VERSION     DAPHBJ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.226"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..603
                     /locus_tag="RHZ76_004032"
     CDS             <1..603
                     /locus_tag="RHZ76_004032"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814567.1"
                     /translation="KIIQDSFLSVIDQLRIRYRTNNQILKLISEITALWTEQYNRFPS
                     PFSWLNYKNKDHCQWLWDEMHKRCIGIPFQPRNQTQRWNFVIATFDNWKGWTIPQEEY
                     LTIKNPKRKPGKLLSGGLDPSNKEIGHKMILLEELKNAWEQKERRARRAKEPTAAKLT
                     KAAQKKLEFIAHLEKSTSKDILNDLINNAFNDAKSKSRQS"
     gene            726..1601
                     /locus_tag="RHZ76_004033"
     CDS             726..1601
                     /locus_tag="RHZ76_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDV2814568.1"
                     /translation="MTKPHGLQAFEQPLASLPDTLRQLILERIQSLTHYEPVIGIMGK
                     SGAGKSSLCNELFRGEVSPVSDVNACTRDVLRFRLRSGRHSLVIVDLPGVGESGQRDH
                     EYRALYRRMLPELDLVLWVMKADDRALSVDEQFWLGVMQPYQQQVLFVLNQADKIEPC
                     HEWDTRTSTPSAQQRVNLQEKQAAIAAMFRPRHPVCVVSACSGWGVEEMVATMMRCLP
                     DRATSPVATQLHGRLCTEPVKSQARNDFGEAVGRVFDTAESSSLLPAPLKTVIRTVRD
                     AVLSVARGVWDWIFF"
     gene            1815..2522
                     /locus_tag="RHZ76_004034"
     CDS             1815..2522
                     /locus_tag="RHZ76_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDV2814569.1"
                     /translation="MTQAERRHDRLAVRLSMIIGRLVAGETLDMCKLAAEFGVSVRTL
                     RRDFRERLMYLDLEYRQGRCRLLSGSRQRELVVMAFARQSGVESLFPELDGHLVSSLL
                     SGSGESPCLIWQGSPSPAVTDSGVFTRLVNAVQECRKVTLRCNGCCYTDVAPYRLVLY
                     DGGWYLTGTHQTRIIALPLADIRAVTSHPDSFTPEANVRDILSRSEFLQALPHFHVFH
                     SLLGDGSYGQLIHKEQV"
     gene            2519..2977
                     /locus_tag="RHZ76_004035"
     CDS             2519..2977
                     /locus_tag="RHZ76_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814570.1"
                     /translation="MKLLTSALMAIAVGFNINHVQADVFSGFQGSIDGGIPAYTRDIN
                     ANNKPYWCTQRKLLQKSCSVVSISSNKRVAIVTSHTGSKACAGGFYGVVDRANGDAMD
                     VFPTKGQLNIDQVCSTDFKVGFIKPTNRNTLASVALYYKGKIVEVLDYSE"
     gene            2997..3467
                     /locus_tag="RHZ76_004036"
     CDS             2997..3467
                     /locus_tag="RHZ76_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814571.1"
                     /translation="MKRYLSVLFALLAFTGVAHAIPNMWASGFGMGVTEYIISSPEKV
                     IFNLNCTGNPDEQNILQHGVLVTLPNGTMLDSHDDGTEITVVMDNSQYPLPSSLGWRN
                     GDNAWVSFIDALGQSSHFDVYVNDNKVGSFSPGLKNTQKELSDLSECRTTHYSD"
     gene            3587..4408
                     /locus_tag="RHZ76_004037"
     CDS             3587..4408
                     /locus_tag="RHZ76_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDV2814572.1"
                     /translation="MRLASRFGRINQIRRDRPLTHEELMSHVPSVFGSDKHESRSDRY
                     TYIPTITILESLQREGFEPFFACQTKVRDQSKREHTKHMLRLRRAGQLTGHQVQEIIL
                     LNSHDGSSSYQMLPGLFRGVCTNGLVCGQSFGEVRVPHKGNVVEKVIEGAYEVLGVFD
                     RVEEKRDAMQSLALPEPARNALANAALKYRFGEDHQPVTVSQLLTPRRREDYSDDLWT
                     VYQRVQENLMKGGLSGRTAQGKSSRTRAVIGIDGDVKLNRALWVMAENMLDFFGR"
     gene            4439..4882
                     /locus_tag="RHZ76_004038"
     CDS             4439..4882
                     /locus_tag="RHZ76_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDV2814573.1"
                     /translation="MNTQNFEFAEQAAITASVVPDELRIGFWPQHFGSIPQWITLEPR
                     IFGWMSRLCVDYHGGIWNFSTLSNGGAFMAPESEHDKTWALFNSMNGNGAELTGEAAG
                     IVACLMAYSHHACRTECDAMTEHYYRLRDFALHHPECSAIMHLID"
     gene            4895..5437
                     /gene="radC"
                     /locus_tag="RHZ76_004039"
     CDS             4895..5437
                     /gene="radC"
                     /locus_tag="RHZ76_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDV2814574.1"
                     /translation="MNTELSPLSSQPELFPLAVMTQHGCLLPATSGLTPYAQRTIRRA
                     INLLDKYLRQPGIAFTSSTAARDWLRLQLAGQEREVFMVLYLDNQHCLLESETLFAGS
                     INHVQVHPREVVKSALRFNAAAVVLAHNHPSGDPEPSQCDRNITGRLKEALALVDVNT
                     LDHLVIGSEGIVSFAERGWI"
     gene            5434..5655
                     /locus_tag="RHZ76_004040"
     CDS             5434..5655
                     /locus_tag="RHZ76_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019106756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 family protein"
                     /protein_id="HDV2814575.1"
                     /translation="MKIISKRQAMTLYRQHPGSRLFRFSTSKHKWSGSICHYAGREVE
                     DIRGVLTVFAERRQDRNGPYVILRSVTLN"
     gene            5681..6037
                     /locus_tag="RHZ76_004041"
     CDS             5681..6037
                     /locus_tag="RHZ76_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDV2814576.1"
                     /translation="MNNHSESGAIPENSSCQQWGLKSTITPCFGARLVQEGDRLHFLA
                     DRAGFNGAFSNEHALRLDQAFPLILKQLELILTSGELSPRHQHCVTLYHNGLTCEADT
                     LGSCGYVYIAIYPTQR"
     gene            6058..6381
                     /locus_tag="RHZ76_004042"
     CDS             6058..6381
                     /locus_tag="RHZ76_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDV2814577.1"
                     /translation="MKFLPTTNMRAAKPCLSPVTIWQMLLSRLLEQHYGLTLNDTPFC
                     DETVIQEHIDAGITLANAINFLVEKYELVRIDRRGFSWQEQTPYLTIIDIMRARRDLG
                     LMNRN"
BASE COUNT         1587 a         1670 c         1681 g         1600 t
ORIGIN      
        1 aaaattatac aagatagttt tcttagtgtt attgatcaat tacgcatcag atatagaact
       61 aataatcaga tcctaaaatt aatatctgaa atcactgcct tatggacaga gcaatataac
      121 agattcccgt cccctttttc ctggcttaat tataaaaaca aagatcattg ccagtggctt
      181 tgggatgaaa tgcataaacg ttgtattggc attccatttc aaccccggaa tcaaacccag
      241 agatggaatt ttgttatcgc gacattcgat aactggaaag gctggacaat acctcaggag
      301 gagtatttaa caataaaaaa tccaaagcga aaaccaggaa agctactatc tggcggtctg
      361 gatccaagca acaaagaaat tggacacaaa atgatcctgc ttgaagaact caaaaatgca
      421 tgggagcaaa aggaaagaag agccagaaga gcgaaggaac caaccgctgc gaagttgaca
      481 aaagcggctc aaaaaaagct tgagtttatt gcgcacttag agaaaagcac atcaaaggac
      541 attcttaatg acctgattaa taatgctttt aatgatgcga agagtaaatc ccggcaatca
      601 taaagcaaca tgatggtgac aataagcttc attcaaaata atttcagaca gatattatct
      661 acgtgaatcc cccgcctcct tatccggtga gcggggattt ttgtttttca cgctgagaac
      721 cctgtatgac taaacctcac ggccttcagg ccttcgaaca accgctggcg tcgctgcccg
      781 atacgcttcg ccagcttatt cttgaacgca ttcagagcct cacccactac gagccggtga
      841 ttggcatcat gggcaaaagc ggggccggta agtcctcgct ctgtaatgaa ctcttccggg
      901 gtgaggtctc ccccgtcagc gatgtaaacg cctgtacccg cgacgtactg cgattccggt
      961 tacgcagcgg gcgtcacagc ctggtgattg tcgatttgcc gggcgtggga gaaagcggac
     1021 agcgggacca tgaatatcgc gccttgtacc ggcgtatgct accagaactg gacctggtgc
     1081 tgtgggtcat gaaggctgat gaccgggcgc tgtcggtgga cgagcagttc tggctcggag
     1141 taatgcagcc ttatcagcaa caggtgctgt ttgtgctgaa ccaggccgac aaaatcgaac
     1201 cctgccacga atgggatacc cgtaccagca cgccttcagc acagcaacgg gttaatcttc
     1261 aggaaaagca ggccgccatt gctgcgatgt ttcgcccccg tcatccggtc tgtgtggtgt
     1321 cagcctgtag tggatggggc gttgaggaga tggtggcaac catgatgcgc tgcctgccgg
     1381 atcgagccac cagtccggta gcgacgcaac tgcacgggag gctatgcaca gagccggtaa
     1441 agtcgcaagc ccgtaacgat ttcggtgaag cggtggggag ggtgtttgat acggcagaat
     1501 catcgtcgct cctgcccgct cccctgaaaa ctgtgattcg taccgtacgt gatgctgtgt
     1561 tatcggttgc ccggggcgtc tgggactgga ttttcttctg acactatgca ggcaaggcaa
     1621 taatgcgagg acataccctg tccgtttatg gcggatttac accgggtaaa gagctgaatt
     1681 cccgggcaga ccgcgccctt cctcgaatca accgatttaa aaaatcgtct ttctgtgtcc
     1741 ataccctgcc ataccccctt gttacactaa tccgcatctt ttcagtgaat tagcttaccg
     1801 gagaacgaag tcttatgact caggctgaac gtcgccatga ccggcttgcg gtcagactgt
     1861 cgatgattat tggtcgtctg gtggcgggtg aaacgctgga tatgtgcaaa ctggccgccg
     1921 agtttggtgt gtcggtccgc accctgcgcc gggatttccg cgaacggctg atgtatctgg
     1981 acctcgagta tcgtcagggc cggtgccgtc tgctgtccgg cagtcgccag cgggaattgg
     2041 tagtgatggc ttttgcccgc cagtcaggcg tcgaatcgct gtttccggaa ctggatggtc
     2101 atcttgtcag ttcacttctg agtggctcgg gcgaatctcc ctgcctcatc tggcagggga
     2161 gcccttctcc ggcagtcacc gatagtggag tctttacccg gctggtgaat gccgtccagg
     2221 aatgcaggaa agtgaccctg cgatgtaacg ggtgttgcta tacagatgtc gctccctacc
     2281 gactggttct gtatgacggt ggctggtatc tgacaggaac gcaccagact cgcatcatcg
     2341 cgctgccact ggcggatatt cgggctgtca cttcccatcc cgacagtttt actcctgaag
     2401 ccaatgtgcg ggacatcctg tctcgctccg aatttttaca agcgctacct cattttcatg
     2461 tttttcattc actgctcggt gacggcagtt acgggcaact tatccataag gaacaggtat
     2521 gaaattactc acttcagcct taatggctat tgccgttggg tttaacatca accatgtgca
     2581 ggctgatgtc ttcagtggct tccaggggag tatcgatggc gggatccctg cctacactcg
     2641 cgatatcaat gcgaacaata agccttactg gtgtacccag cgtaagttgc tgcagaagag
     2701 ttgttccgtt gtctccatct cttccaataa gcgggttgcc attgttacca gccacactgg
     2761 tagtaaggcc tgcgcaggtg gcttctacgg cgtcgtagac cgtgctaacg gtgacgcgat
     2821 ggatgtcttt ccaaccaaag gtcaactgaa catcgaccag gtctgttcta ccgattttaa
     2881 agtgggcttt attaaaccta ctaaccgtaa caccctggcg tctgttgctt tgtactacaa
     2941 aggcaaaatc gtcgaggttc tcgactattc agaataaaat aaaaagaggg ggtgtcatga
     3001 aacgttactt atctgtcttg ttcgccttac tggcattcac tggcgtcgca cacgcgatcc
     3061 ccaatatgtg ggccagcggt tttggtatgg gggtgacgga atacattatc agtagccctg
     3121 agaaggtgat ttttaacctc aactgtactg gtaatcccga tgaacagaac attctgcagc
     3181 atggcgtatt agtaaccctg cccaatggta cgatgctgga ttctcacgac gacgggacag
     3241 aaataacggt ggtcatggat aacagccagt atccgttacc ttcgtccctc ggctggagga
     3301 atggcgataa cgcctgggtg tcatttattg acgcgctggg tcagtcgtct catttcgatg
     3361 tctacgtcaa cgataacaaa gttggctcct ttagtccggg cctgaaaaat acgcaaaaag
     3421 agctgtctga cctcagtgag tgtcggacca cacactacag cgattaataa ttcacatttc
     3481 agacctctcc agcccgctac gcaccaacac cagatagcaa caggccatgc tccttcgaga
     3541 acatggcctt tttgttttta tttcattatc agataaggag ttagccatgc gattagccag
     3601 ccgctttggc cgtataaacc agatacgccg tgaccggcct ctgacccacg aagagctgat
     3661 gagtcatgta cccagcgtgt tcgggagtga caagcatgaa tctcgttcag acaggtacac
     3721 ctacatcccg accatcacca tcctcgaaag cctgcagcgt gaaggctttg agccgttctt
     3781 tgcctgccag acaaaggttc gcgaccagag caagagggag cacaccaaac acatgcttcg
     3841 cctgcgtcgt gccggtcaac tgaccggcca tcaggtgcag gaaatcattc tacttaacag
     3901 ccatgacggc tcatcaagct accagatgct gccggggtta tttcgtggcg tctgtaccaa
     3961 tggcctggtc tgcggccagt cattcggaga agtgcgggta ccgcataaag ggaatgttgt
     4021 tgagaaggtg attgaagggg cttacgaagt gctcggtgtg tttgaccggg tggaggagaa
     4081 gcgcgatgca atgcagtctc tggcactgcc agaaccagcc cgtaacgccc tggcgaatgc
     4141 cgcgctgaag tatcgctttg gtgaagacca ccagccggtc acggtatcgc agttgcttac
     4201 cccacgtcgt cgggaggact acagcgatga cctgtggacc gtataccagc gcgtgcagga
     4261 gaacctgatg aaagggggat tgtcagggcg aactgctcag gggaaaagca gccgcacccg
     4321 tgctgttatc ggtattgatg gcgatgtgaa gctcaatcgc gccctgtggg taatggcaga
     4381 aaacatgctc gattttttcg ggcgttaaac aaccgctccg gcataaggag atagtttaat
     4441 gaatacacaa aactttgagt tcgctgagca ggcagcaatt actgcctcgg tagtccctga
     4501 cgagttacgc atcggcttct ggccgcagca ttttggctcc atcccacaat ggataaccct
     4561 tgagccccga atttttggct ggatgagccg tctgtgtgtc gattaccacg gtggtatctg
     4621 gaacttttcg acgctcagca acggcggtgc tttcatggct cctgagtcag agcacgataa
     4681 gacgtgggca ttgttcaaca gcatgaacgg caacggcgct gaacttactg gcgaagcggc
     4741 agggatagtc gcctgtctga tggcatacag ccaccacgcc tgtcgtaccg aatgtgatgc
     4801 gatgactgag cactattatc gcttacgtga cttcgcgcta caccatccag agtgcagcgc
     4861 cattatgcat ctcatcgact gaggaatgcc tgacatgaat accgaattat ccccgctgtc
     4921 atctcagcct gaactctttc cgctggcggt tatgacccag catggttgcc tgctcccagc
     4981 gacatccgga ttgacacctt atgcccaacg gaccatccgc cgggctatca atttgcttga
     5041 taaatatctg cgccagcctg gaatagcctt tacctcaagt actgctgccc gtgactggct
     5101 tcggttacag ctggccggac aggagcggga agtgtttatg gtgctctatc tcgataacca
     5161 gcactgttta ctggagagcg aaacgctgtt tgccggttcg attaaccatg tccaggtcca
     5221 tccccgtgag gtggtgaagt cagcgcttcg cttcaatgct gcagctgtcg tactggcgca
     5281 taaccatcca tccggtgacc cggagcccag ccaatgcgac cgcaacatta cgggcaggct
     5341 taaagaagcg ctggcactgg ttgatgtgaa cacgctcgat catctggtca tcggttcaga
     5401 gggcattgtg tcgttcgctg agcgaggctg gatatgaaaa tcatcagcaa acgccaggca
     5461 atgaccctgt accggcaaca tcctggctcc cgactgtttc gcttcagtac gagcaaacat
     5521 aaatggtcag gtagtatctg ccattatgct ggccgcgagg tggaggacat acgcggcgtt
     5581 ctgaccgtgt ttgctgaacg tcgtcaggac cgcaatggtc cctatgtcat tcttcgaagc
     5641 gttactctca attaatcctt cactttaatc agaaacaata atgaacaatc actcagaatc
     5701 cggtgccata ccggagaatt cctcctgcca gcagtggggg ctaaagagta cgatcacacc
     5761 ctgttttgga gcacgtctgg tgcaggaagg cgaccgcctg cattttctcg ctgaccgcgc
     5821 cgggtttaac ggtgctttca gcaacgagca tgcgttgcgc ctcgaccagg cctttccgtt
     5881 gatactcaag cagctggagc tgatactcac cagcggagaa ctctcccccc gccatcagca
     5941 ctgtgtcacc ctctatcaca acggactcac ctgcgaagcc gatacgcttg gtagttgtgg
     6001 ctacgtatac atcgccattt atcccactca acgttaatta ctttcacgag agaaaaaatg
     6061 aaatttctac ctacaacaaa tatgcgggcg gcgaagccat gcctgtcgcc cgtgactatc
     6121 tggcaaatgc tacttagtcg tctgctggaa cagcattatg gcttgacact aaacgataca
     6181 cctttctgtg atgaaaccgt catacaggaa catatagatg ccggtatcac tctcgctaat
     6241 gccattaact ttctagtcga aaagtacgaa ctggttcgta tcgatcgcag agggtttagc
     6301 tggcaagaac aaacgccata tctcacgatt attgatatca tgagggcccg tcgcgatctg
     6361 ggtttaatga atcgtaatta gtctgatgct aacggtatcg ctgcttttga gtatgcctgc
     6421 catttggtat catctcgctt cctgctgatt tatcgataaa ggtaattgct tgaattcaaa
     6481 gcctctgttc tttgggggta tctttggggg tataagtaaa gttataactt gaaaattt
//
LOCUS       DAPHBJ010000225         6528 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.227, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000225 DAPHBJ010000000
VERSION     DAPHBJ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.227"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(1..1008)
                     /locus_tag="RHZ76_004043"
                     /pseudo
     CDS             complement(1..1008)
                     /locus_tag="RHZ76_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611476.1"
                     /note="programmed frameshift; incomplete; partial in the
                     middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSen4 family transposase"
     gene            complement(1175..1519)
                     /locus_tag="RHZ76_004044"
     CDS             complement(1175..1519)
                     /locus_tag="RHZ76_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814578.1"
                     /translation="MELGIMVCIVLLLSLAAVWISNKTVGLLEIHYFKRPLSMEYAAW
                     LRVMCAGLLFIFIQMVPAFTFLYTLKLVALLNLGVQTMKLNGVYKFKRTGMMPPKDAP
                     HDLGRFNPFRKK"
     gene            complement(1455..2363)
                     /locus_tag="RHZ76_004045"
     CDS             complement(1455..2363)
                     /locus_tag="RHZ76_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814579.1"
                     /translation="MQWISTTSQKLDERLYRVCVWVKKYHEDAINRVVLTVDLSKARH
                     DPIEDQVRAELLCGHYFLMRKENNAKSAPADQNFEFLPDGRNLSWQTSTPGLQHLLLN
                     SNVPESLRPLADYIQMRLAKLTMVPMSGTIMKAALEDSISWVKVDLRYYWQYELVNSH
                     LGPVQVTHKALVRFGRLAKHDENASAIRMLRQRLSSPFIQEFEMPGEELKRKQKIMNT
                     VDVKMLFHTHYPGQKLLLARYANGWVLVDCFLFHHIKPKKKAKNKTKAGKSETQNIKS
                     TGSEDHGTGHYGVHRVVTLTGSGVDQ"
     gene            complement(2374..3333)
                     /locus_tag="RHZ76_004046"
     CDS             complement(2374..3333)
                     /locus_tag="RHZ76_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HDV2814580.1"
                     /translation="MASEDLLFYRDRILERIEKLRSFLNDHQPVMAELMTVGTVTRHE
                     ERLKEVNPIEVSTISDASVDEIIRVIQDFCIDDIDVLSRNSTKVTTKYPGLIIVPEGA
                     EQLSGIICDINDAKSDFASAMKRVNSEKHIRFKEVHRKLPGLVTAHSTRKILFVEEQL
                     KKVTFAWRLNRNQVKTNASDLIAMLDKRRLVAVKSPVTTDLTVVANIDRAKALLERKV
                     LRDGEGYRLCRTNTFPVPIAHLFTYRPEGKERGSSKYAETDYKVVKASLPIFGVGQKP
                     TIKRLQDWVPAQDEALSNGRRSNHSYTELVPGADLGIFIMRKV"
     gene            complement(3422..3697)
                     /locus_tag="RHZ76_004047"
     CDS             complement(3422..3697)
                     /locus_tag="RHZ76_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814581.1"
                     /translation="MHLLVEGKGVIPDVSVSDLGNVQNIRNELIGLDWWVKFGVKQDW
                     QRINLQRQPVSFGLLNDPNRCFRHDGQQVEPLPLYTTGNPGELRKVL"
     gene            complement(3915..4307)
                     /locus_tag="RHZ76_004048"
     CDS             complement(3915..4307)
                     /locus_tag="RHZ76_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814582.1"
                     /translation="MVFWIIISFMFLSAMLMTGHLLNKVVLQPVWLCIWSLPWLGFLL
                     KVRVTEMDKGLVNTLQLGDLPVFRWSRSVVPGTWSVDRINGRYRLNRIGTDGLTVHYP
                     WRLNYQPLFLTPDKQISDINGREHERKF"
     gene            complement(4387..4923)
                     /locus_tag="RHZ76_004049"
     CDS             complement(4387..4923)
                     /locus_tag="RHZ76_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814583.1"
                     /translation="MKSKIHSSGTSGTKRVLKTDIALPLLCWVFTSPFSNWTDKFFTG
                     TEVPEGSLPGLEQAPEAIFRFVLNDEGFDVGFDAVGMDLCCFSIPLSTMPTKNLDDEE
                     TLSRLTGDVIHGVLLSLPEYIEMPDRLVYQLTDEVMAFNSHCGNGILHGWTTAQELWR
                     NEILPRTTILMQQTSVIH"
     gene            complement(4997..5254)
                     /locus_tag="RHZ76_004050"
     CDS             complement(4997..5254)
                     /locus_tag="RHZ76_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814584.1"
                     /translation="MSNTDSQTVPADALCENSSQVKIQLSRSASRALTTAKEILETRG
                     LVSVSTEDLVQACLEENQPLDLASIYLEHRLKQKGTSICEF"
     gene            complement(5262..5852)
                     /locus_tag="RHZ76_004051"
     CDS             complement(5262..5852)
                     /locus_tag="RHZ76_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814585.1"
                     /translation="MMTESFFDWVVYCITGVILASAFLEGKRTRFPAAFFYGSIVLLV
                     VLIVAGALNWWFYRSKFGSVVVIFAATTLLMTRFRLVIYAFRFRTLVADLQSHVAKGF
                     RIILVLPENEDEKTVLLSKLSKVIHSGTLFYTRTALGPYSGDLLHALGQKHRNGEGYL
                     LLCEQQLPARTWLSTVENGQPEKSIAVNFHSIPDME"
     gene            complement(5907..6176)
                     /locus_tag="RHZ76_004052"
     CDS             complement(5907..6176)
                     /locus_tag="RHZ76_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814586.1"
                     /translation="MPATTILSNNPVNSVFSNDNSHFTYRHIDFSIELLLSSGTDSQL
                     EYLFLKPAAEVKIDLQRRLASGERYVTTESCDNFSEVDGCMGHDC"
BASE COUNT         1721 a         1697 c         1472 g         1638 t
ORIGIN      
        1 agcccgctgc aaattcagac ggtgtctgat aattcagcgt ggagtgcggg cggcattcgt
       61 tataatcctg ccgccagtca ttaataattt tcctggcatg aacgatatcg ctgaaccagt
      121 gctcattcaa acattcatcg cgaaatcgtc cgttaaagct ctcaataaat ccgttctgcg
      181 ttggcttgcc cggctggatt aagcgcaact caacaccatg ctcaaaggcc cattgatcca
      241 gtgcacggca agtgaactcc ggcccctggt cagttcttat cgtcgccgga tagcctcgaa
      301 acagtgcaat gctgtccaga atacgcgtga cctgaacgcc tgaaatccca aaggcaacag
      361 tgaccgtcag gcattccttt gtgaaatcat cgacgcaggt aagacacttg atcctgcgac
      421 cggtggaaag tgcgtccatg acgaaatcca tcgaccaggt cagattgggc gccgccggac
      481 ggagcagcgg cagacgttct gttgccagcc ctttacgacg tcttctgcgt tttacgctca
      541 ggccactgag gtgataaagc cggtacacgc gcttatgatt aacatgaagc ccttcacggc
      601 gcagcaactg ccaaatacga cggtagccaa aacgcctgcg ctccagtgcc agctcagtga
      661 tgcgccctga taaatgcgca tcagcagccg gacggtgagc ctcatagcgg caggtcgaca
      721 gggataaacc tgtaagcctg caggcacgac gttgcgacag accggtcgca tcacacatca
      781 acatcacggc ttcccgcttc tggtctgtcg tcagtacttt cgcccaagag ccacctgaag
      841 cgcctcttta tccagcatgg cttcggcaag cagcttcttg agtctggcgt tctcttcctc
      901 aagcgacttc aggcgcttaa cttcaggcac ctccataccg ccatacttct tacgccaggt
      961 gtaaaacgtg gcatcggaaa tggcatgctt gcggcagagt tcacgggcgg gtaccgccga
     1021 aggactcggc gcaaataaaa aacgtacata ccattataca cagtatgtac gtttgaaatg
     1081 aaagccgctg acgttatatg taaagccctg tcactcactc acacgcgaat agacactgtt
     1141 tttactatat actccttcgg ggcgctaatg aggattactt ctttcgaaat ggattaaagc
     1201 ggccaagatc atgaggggcg tctttcggcg gcatcatccc ggtgcgttta aatttataaa
     1261 ccccgtttag cttcatcgtc tgcacgccca gatttaacag tgccaccagc ttcagggtat
     1321 acagaaacgt gaatgccggg accatctgaa taaaaataaa tagcaaaccg gcacacatca
     1381 cacgcagcca ggctgcatac tccatgctca gcggccgctt aaagtaatgg atttccagca
     1441 atccgaccgt cttgttactg atccacaccg ctgccagtga gagtaacaac acgatgcaca
     1501 ccataatgcc cagttccatg atcctcactc cccgtcgatt tgatattttg agtttctgat
     1561 ttacccgctt tggttttgtt tttcgccttc tttttgggtt tgatatggtg gaacaggaaa
     1621 caatccacca gcacccaacc attagcatat cgggccagca gtaatttctg gcccggataa
     1681 tgtgtgtgga aaagcatttt cacatcaacg gtattcatga tcttctgctt gcgcttcagc
     1741 tcctccccag gcatctcaaa ttcctgaata aacggtgatg acaaccgctg gcgaagcatt
     1801 cgaattgcac tagcgttttc atcgtgtttt gccagcctcc cgaatcgcac aagcgcctta
     1861 tgagttacct gaacaggccc cagatgggaa ttaaccagtt catactgcca gtagtatcgc
     1921 agatccactt ttacccagct gatgctgtct tccagtgcgg ctttcatgat ggttcccgac
     1981 atcgggacca tggtcagctt ggccagacgc atctgaatgt aatcagccag gggcctaagg
     2041 gactcaggta cattgctgtt caggagaagg tgctgaagcc ccggtgtcga cgtctgccag
     2101 ctcaggttgc ggccgtcagg caaaaattca aagttctgat ccgctggcgc cgattttgcg
     2161 ttgttctctt tccgcatcag aaaataatgc ccacacagta actcggcccg tacctggtcc
     2221 tctatcgggt catggcgggc cttactgaga tcaaccgtca acacaacacg gttgatggca
     2281 tcctcatggt attttttcac ccagacacag acacgataaa gtctctcatc aagtttctgg
     2341 ctggtcgtgg aaatccattg catgcttttc tcctcagacc tttctcataa tgaaaatgcc
     2401 aagatctgca ccgggtacaa gttccgtgta gctatggttg ctccttcgac cattggacag
     2461 tgcttcatcc tgcgctggca cccagtcctg aagtctttta attgttggct tttgtccgac
     2521 gccaaaaatg ggtaatgagg ctttcaccac cttgtaatca gtctcggcat atttgctact
     2581 tccacgctcc ttgccttcag gccggtatgt gaacaaatgc gctatcggaa ccgggaaggt
     2641 attggttcga caaaggcgat acccttcgcc gtctctcagt acttttcttt ccagtaatgc
     2701 cttcgctcta tcgatgttcg ccaccactgt caggtcagtt gtcacgggcg atttgaccgc
     2761 tactaaacga cgcttatcca gcatcgcgat gagatcactt gcgtttgttt ttacctggtt
     2821 tcggtttagc cgccaggcaa aagtaacttt tttgagctgt tcttccacaa acagaatttt
     2881 gcgagtggaa tgcgcagtca ccagaccggg aagcttacgg tgaacttcct taaaccggat
     2941 gtgtttctcc gaattcaccc ttttcattgc agaggcaaag tcacttttgg catcatttat
     3001 atcgcaaata attccgctca gctgttcggc accctcagga acaataatca accccgggta
     3061 tttggttgtc actttcgtcg aattcctgct gagtacatca atgtcatcga tacagaaatc
     3121 ctgaataaca cggataattt catccacgga ggcatctgaa atagtactca cctcgatggg
     3181 gttcacctct ttcaaccgct cttcgtgtcg tgtgacggtc cccacagtca tcagctcggc
     3241 catcaccggc tgatggtcat tcagaaagga tctgagcttt tcaattcgct ccagaatccg
     3301 gtcacggtaa aagagtaaat cttcactggc cattcatcac tccggtttca ataaagcagc
     3361 gcaacaccgt tacgtcttca acgacttctg aagagtcgat atgcagccgg tagttgtact
     3421 tttacagcac cttccgcaat tccccgggat tgccggttgt ataaagtggc agaggttcaa
     3481 cctgctgacc gtcatgccga aaacaacgat tagggtcatt caaaaggcca aaactgaccg
     3541 gctggcgttg cagattgatg cgctgccagt cctgctttac accaaacttc acccaccaat
     3601 ccagaccaat cagttcatta cgtatgttct gcacattgcc caaatccgat actgaaacat
     3661 ccgggattac acccttccct tccaccagca aatgcatcac gctctaaaaa tcaggctgac
     3721 ggctcttcct tgcgtgcttc agaattgtac gaatcgaacg caagctgtgg cgggaatgca
     3781 aattacgacg ttcagccgaa ctaagccatc tctggataag ctcatttgaa tatgacaccg
     3841 gcatttcacc ggggcctgac ctttcgattt cacgcacata cagcaggcag gaagtagcgg
     3901 cactcactac tgcgtcagaa ttttcgttca tgttcacgtc cgttaatatc agaaatctgc
     3961 ttatccggtg tcaggaacaa aggctgatag ttcaggcgcc acggatagtg taccgtcaaa
     4021 ccatccgttc caatccggtt caaccggtag cgtccattaa ttctgtcaac agaccaggtg
     4081 ccaggaacta cagaacgact ccagcggaaa accggcagat ctccgagctg aagtgtattc
     4141 acgagccctt tatccatctc ggtcacgcgt accttcaaca agaagcccag ccagggtaaa
     4201 gaccagatac acagccaaac gggctggagg acaactttgt ttaacaaatg acctgtcatg
     4261 agcatcgctg ataaaaacat aaaactgatg ataatccaga acaccatcca tcctaaccag
     4321 gctcttgtct cacgaagttt ccattcaaga aggtaaagtc gctttttcat ccagagcccc
     4381 cggaactcag tgaatgactg atgtctgctg catcaaaata gtggtgcgtg gcaggatctc
     4441 gtttcgccac aactcttgag ccgtcgtcca tccatgtaga attccgttgc cacagtgtga
     4501 attgaacgcc atgacctcat cagttagctg atagaccagc ctgtctggca tttcaatgta
     4561 ctccggaagc gaaagcagga cgccgtgtat cacgtcgccg gtaagcctcg acaacgtctc
     4621 ttcgtcgtcc agattctttg tgggcatcgt cgaaagcggg atagagaaac aacagagatc
     4681 cattcccacc gcgtcaaaac caacatcaaa accttcgtca ttgagcacaa atcggaagat
     4741 agcctcaggc gcctgttcta gacccggcag cgagccctcc ggcacttctg tgccggtaaa
     4801 aaacttatca gtccagttac tgaatggact ggtgaacacc caacaaagca gaggcaacgc
     4861 aatatccgtt ttaagtactc gtttcgtgcc ggacgttcct gaagagtgaa ttttgctttt
     4921 cattatttac ctgtagagtg ctttacttac agctatataa acacattccg gaatttcccc
     4981 aataggttgg aaaacactaa aattcacaga tactggttcc cttctgtttt agccggtgct
     5041 caagatagat acttgccagg tcgagtggct gattttcttc gagacaggcc tgaacaaggt
     5101 cttcagtgga tacggatacc agccctcggg tttcaagaat ttctttcgcc gtcgtcaatg
     5161 ctcgtgaggc gcttcgactt agctgaattt tcacctgaga actgttttca cacagggcat
     5221 cagctggtac ggtctgtgaa tctgtattgc tcataggggt atcactccat atcagggatc
     5281 gaatgaaaat taactgcaat agacttctct ggttgaccgt tttctacagt gcttagccag
     5341 gttcgagccg gaagttgttg ctcacaaaga agcagatagc cctcgccatt tcggtgcttt
     5401 tgtcccagag catgcaggag atccccagaa tatggaccca gagcagttcg ggtataaaac
     5461 aacgtcccag aatgtatcac tttcgacaat ttgctaagca aaacggtctt ctcgtcttca
     5521 ttttcgggta agacaaggat tatcctgaat cccttggcta catgtgattg gagatctgcc
     5581 accagcgtcc gaaacctgaa cgcataaata accaatctga atcgggtcat taacagcgtt
     5641 gtcgcggcaa aaataacgac aactgagccg aatttagaac ggtagaacca ccagttgagg
     5701 gcaccggcca ctatcaaaac gaccagcagc acaatgcttc cgtaaaaaaa tgcagccgga
     5761 aaacgggttc tttttccttc gaggaacgca gatgccagta ttacgcctgt gatgcaatac
     5821 acaacccagt caaagaacga ttcagtcatc attaggtgat agtgtctcgt gttgttttaa
     5881 atcggacgcc tgccgttata aagtcctcag caatcgtgtc ccatgcagcc atcaacttcg
     5941 ctaaaattgt cacaactctc ggttgtaacg taacgttccc cactagctaa acggcgctgt
     6001 aaatcgatct ttacttcagc cgccggtttg agaaacagat attccagttg tgagtcagtt
     6061 cctgaagaaa gcagtagctc aatactgaaa tcaatatgcc ggtatgtgaa atggctattg
     6121 tcattagaga agaccgaatt cacagggtta ttgctcagga ttgtagtggc aggcatagtg
     6181 actcctttgt tttaaacagt atacaaatct ctaacgatcc atttttcaga taagagacct
     6241 attttcgcga aaccgaaaca aaagaagtaa aaagtaattt ggaggctcca gcattcggac
     6301 tgtgtgctgc cggaaacgtc agcaaggggc aaaacattcg agtaaaagaa gcagagaagg
     6361 aaacagctgg aaggatgtaa attaggctag gtttggttga agaaggtaat ggaaggctta
     6421 catgtaacgg tgccaagagt acagtaccgt aaaacatata gggcagtaag aacgttgtaa
     6481 aattgtgaaa tcaatgcaag ttttgacgaa agcaaaaaaa aaggcacg
//
LOCUS       DAPHBJ010000226         6389 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.228, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000226 DAPHBJ010000000
VERSION     DAPHBJ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.228"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..3126
                     /gene="trhN"
                     /locus_tag="RHZ76_004053"
     CDS             <1..3126
                     /gene="trhN"
                     /locus_tag="RHZ76_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein TrhN"
                     /protein_id="HDV2814587.1"
                     /translation="NTMSWAFYVSLINIALTPKIFAADAIFDQLENNFNLANPNANRN
                     ATTSAQDIVEKYKNADSGENLSGKISEKYVGKAESTNLNVGKYGTPNSNESVMSNAVS
                     DGKSIGKAVQLPSMSGGTINSNYTKEGAKLLSRDSSGNIGISNNPNTTAGTKTSTGEL
                     FSSEQKHSDVQFNAGGRYGDENGFINDIKNRKSQLFEAQSYDGVAYRTLVNANKENPA
                     STIKPNDPMFNAGRNEIGNAVAGTGNWLQNCNTETSKQTITTHYPDYKEFYCNSPKKD
                     NFNSCTITRDFSVPVYISGGNGDMSMCGDNCVRVWFGRRDDNYWNDGVYDNSLTLKFH
                     PDAKLATAKIINAEWDDHMRVTLDGTQIFAHIDGAYRSSNYPSPQGQWELKKSWKLDK
                     VYDITEQVKTSVYQEQDREVTMASRVWVGGGGEGYFEVEMTFENMKLEDKHIQEPAGC
                     FDAVQTPNSFCRFDRFVNMDVGTKRLPESVLKMATPLYKGDTGYLTWKTNLEGYFCDP
                     LAKEKLCSYDAKGNIMKDPTGKDLCYNYDDIKNMPDACSTYKNDAACVLDNQTCAEGW
                     FDEGTNTCYMYEQKYTCDRGKDVVREVESQTNSCVGMIPCSGGTCETGPKEENKDFGK
                     VAAYSNMVQYMQGEAKCEDPNDPNSCSVFEGKAEWCGRSVGFVNGLAKTDCCEKPQGA
                     AGSLEAIMLAGSMIRNTNWTRVNAQLVNWTGGESGTWASMANSVGEWTASAGKTVGQM
                     WNNVTSSITSVYENVAGNLGRTVGSSAAGESGQLAKETMSSFGLGKLKQMAMEKAYDL
                     LPDTVRDFVFKNVATTGGEIVFSAAVQNFMLALNVIGWIYTAYQVTKMLLEMLVACDQ
                     KEMEASIHKNQKSCFTLDTNRCVKYLNLGFTKKCVKKATDMCCYNSMLSRVIMQQAYP
                     QLGIDPVTSNCVGLSIGQIQKLDFDKIDLTEWINDAVQVGEVPDQYATFSESSITANL
                     PFKNENYQLPSERTKDAMGGEENMIKARQENAQAIKEENVDCSYLPRPAICEVGSTYV
                     DPITGKEIPKY"
     gene            complement(3154..4026)
                     /locus_tag="RHZ76_004054"
     CDS             complement(3154..4026)
                     /locus_tag="RHZ76_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV2814588.1"
                     /translation="MKNKRFVSLVSALVASASLVGCAGNAGQSPNSAQHSDTHGLYWH
                     ANAKDSVAKNAYSLAGFDVSFNEETVQPGGDADNFLSKLLISGSMGYVTGGLSGLSIM
                     SLGSLYSSSDAEYIQVNQYVVFVPNPKKLPYNDESLVRAGAAYVYNHTKESQVMLGFN
                     PSKQSAALASCKIDRAVINKWSTCELTSKPAPDVMPTNSMYSFQAIRPATGTEIPQLN
                     LPAGEYSVIRYVFIPFKGNESSNNFSGIIFRSDSPKFTMPGGAAASINGKDYYLFSGE
                     YGQKGFPEKTLKSK"
     gene            4208..5992
                     /gene="trhI"
                     /locus_tag="RHZ76_004055"
     CDS             4208..5992
                     /gene="trhI"
                     /locus_tag="RHZ76_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer helicase TrhI"
                     /protein_id="HDV2814589.1"
                     /translation="MGKPTDEQRVIIENANANNMVIAAPGSGKSFTMIEAVISILRQF
                     PYAKVGMVTFTRAATNSLAEKLKRRLSKKDQDRVLVNTFHGFIRMQLDMVNWKGKMLI
                     SSAQRSVIHRALKESGAPFRYPDAEFAIDAIGREMDTDIISVRHTRQQIHLFNTYQAI
                     CQKDHVADLNALSRFVVGQMYSGKMQPLNLTHLVVDEVQDTDSIQYAWISLHTRAGVN
                     TSIVGDDDQAIYSFRASGGVKIFQQFEKQFRPNIFYLNTCFRCEPEILKVAGALIEKN
                     VYRYAKDLRSAKGGGGKVHFRSYVDMDEQIQGILNLINQDPIGWAILSRGNAHLDQLE
                     SLIEQPVLRYGGKSFWDEKETSDVLHLMAFFRHSNDVRLMKRVLALFGENEEVLDQTA
                     LSMKGRKVTFGELNIPNESSLETRTLHSNFTRFTQETREKVEIEKRFANLIKWMELSS
                     IKMRTQKGSPSLSRIALDTCKQWAEKTGWQNMINRAAAMCLGPKKKDEEYTPDKVVLS
                     TLHGSKGLEWKNVIIMSCNADQIPSKRSVGQEAIEEERRLLFVGFTRAEQQLHVMWYG
                     DPSFFLSECAEDKLKEAAKSRTESTLTE"
BASE COUNT         2006 a         1217 c         1551 g         1615 t
ORIGIN      
        1 aatactatga gttgggcatt ttacgtgtcg ctcataaaca ttgcgttaac gcctaaaata
       61 tttgcggcag acgcgatttt tgaccagctt gaaaacaatt ttaaccttgc caatcccaac
      121 gccaaccgga acgcaaccac cagtgctcag gatattgtgg aaaagtataa aaatgctgat
      181 tccggggaaa atctgagcgg caaaatttcc gagaaatatg ttggtaaagc agagtcaaca
      241 aacctaaatg ttgggaagta cgggacgccg aactcaaatg aaagtgttat gagtaatgcg
      301 gtgtccgacg ggaaatcgat cggtaaggct gtacaattac ctagcatgtc gggcggtaca
      361 attaactcga attatacgaa agagggggca aagttacttt ctagggacag cagcggtaat
      421 attggaataa gtaacaaccc gaatacaacg gcaggtacca aaaccagtac aggggaacta
      481 ttcagctcag agcagaagca cagtgatgtt cagttcaatg ctggtggccg gtatggagat
      541 gaaaatggct tcatcaatga catcaaaaat cgaaaaagtc agctgtttga agcgcaaagt
      601 tatgatggag tcgcctaccg tactctggtg aatgcaaata aagaaaaccc agcatctact
      661 attaaaccta acgaccctat gttcaatgcg ggtcgaaacg aaataggaaa tgctgtagct
      721 ggtacaggaa actggctaca gaattgtaat acggaaacat caaaacaaac aattactacg
      781 cactatccag attacaaaga gttttactgc aattcaccca agaaagataa ctttaacagt
      841 tgcacgataa ctcgtgattt ttccgtgccg gtttatatct caggtggtaa tggtgatatg
      901 tccatgtgcg gagataattg tgtgagagtc tggtttggca gaagggacga caactattgg
      961 aacgatggag tctatgacaa ctcattaacc ttaaaattcc atccagatgc aaagctcgca
     1021 actgcgaaaa taattaacgc agagtgggat gaccatatga gggtcacgct tgatggtaca
     1081 cagatttttg ctcatataga tggagcatat agaagtagca attatccatc tccacaaggt
     1141 caatgggagt taaagaaatc ctggaagctc gataaagtct acgatattac tgaacaagta
     1201 aaaacctctg tatatcagga gcaggatcgt gaagtgacta tggcttcgcg cgtatgggtt
     1261 ggcggtggtg gggaaggcta ttttgaagtt gagatgactt ttgaaaatat gaagttggag
     1321 gataaacata tacaggagcc cgcaggatgt tttgatgcag tacaaacgcc gaattcattc
     1381 tgtcggtttg acaggtttgt caacatggat gttggtacaa agcgactccc ggaaagtgtg
     1441 cttaagatgg ccacaccgct ttacaaaggt gatacaggat acttaacctg gaaaacaaac
     1501 cttgaaggct atttctgtga tcccctggcg aaggaaaaac tgtgctctta tgatgcgaag
     1561 ggaaacatca tgaaagaccc cacaggcaaa gacctttgct acaactacga cgatataaaa
     1621 aatatgcctg acgcatgcag cacatataaa aatgatgctg cgtgtgtgct agataatcag
     1681 acatgtgctg aaggttggtt cgatgaaggc acaaatactt gctacatgta tgagcagaaa
     1741 tatacatgtg acagagggaa agacgtagtc agagaggtag agtcacagac taactcctgt
     1801 gtaggtatga tcccatgttc tggcggaaca tgtgaaacag gcccaaaaga agaaaacaaa
     1861 gattttggga aagtggccgc gtactccaat atggttcagt acatgcaagg tgaagcaaaa
     1921 tgcgaagatc caaacgaccc taactcctgc tctgtctttg aggggaaagc agagtggtgc
     1981 ggcaggtccg ttggttttgt gaacggactg gccaaaacag attgctgcga aaagccgcag
     2041 ggtgctgctg ggtcactgga ggcgataatg ctggccgggt cgatgatccg aaacacgaac
     2101 tggacccgag tgaatgctca actggtaaac tggactggag gggagagcgg aacctgggca
     2161 tcaatggcta actcagtggg tgaatggact gcatcggcag gtaaaactgt cggacaaatg
     2221 tggaacaatg tgaccagttc aataaccagc gtttacgaaa acgtggccgg taaccttggc
     2281 cgaacagtag gatcatccgc tgctggtgaa agtggtcagc ttgctaagga gactatgagt
     2341 tcattcgggc ttggtaaact taagcagatg gccatggaaa aagcctatga cctgcttccc
     2401 gatacagttc gagacttcgt atttaaaaat gtagcgacaa cgggtggaga aatagtcttt
     2461 tctgctgctg ttcaaaactt tatgcttgca ttaaatgtaa tcggctggat ttatacggca
     2521 tatcaagtca caaaaatgct gcttgaaatg ctggtggcct gcgatcaaaa agaaatggaa
     2581 gcatctattc acaaaaatca gaaatcctgt tttacactgg atacaaaccg ttgtgttaaa
     2641 tatctgaatc tgggatttac aaagaaatgt gttaaaaaag caacagacat gtgctgttac
     2701 aattcaatgt tatctcgcgt tattatgcag caggcctacc cgcagcttgg aatagacccg
     2761 gttacttcaa actgcgttgg cttatcaata gggcagatcc aaaagttaga ttttgacaag
     2821 attgatttaa cagaatggat taacgatgct gttcaggtcg gagaagtacc ggaccagtac
     2881 gctacattct cagaatcgtc aataacagca aatttgccat ttaaaaacga gaattatcaa
     2941 cttccatcag aaagaacaaa agatgcaatg ggaggagagg agaatatgat aaaagccaga
     3001 caggaaaacg ctcaggccat taaagaggag aacgtagact gtagctatct tccacgccct
     3061 gcgatttgtg aagttgggtc aacttatgta gatccaatta caggtaagga aataccaaaa
     3121 tattaaaaaa aaggggctgt aaagcccctt ttgttatttt gatttcagag ttttttccgg
     3181 gaatcctttc tgaccatact caccagagaa gaggtaatag tctttcccat taattgaagc
     3241 agctgcgccg cctggcatgg tgaactttgg actatcagaa cggaaaataa tacctgagaa
     3301 attattgctt gactcattgc ctttaaatgg aatgaacacg taacgaataa ctgaatactc
     3361 accggcaggt agattaagtt gggggatttc agttccagtc gcagggcgaa tagcctggaa
     3421 gctgtacatg gaattagttg gcattacgtc aggagctggt tttgaagtga gctcgcatgt
     3481 actccattta ttgatgaccg ccctatcaat cttgcaggac gccagagctg cactttgctt
     3541 cgatgggttg aatccgagca ttacctgtga ctctttggtg tgattgtaaa catacgcagc
     3601 accggcacga actaggcttt catcgttata aggaagtttc ttcgggttcg ggacaaagac
     3661 aacatactgg ttaacctgaa tgtattcggc gtcagaggag ctgtatagag agcccaaact
     3721 cataatgctt aatccgctga gaccgcctgt cacatatccc atagagccgc taatcaagag
     3781 tttggaaagg aagttatctg catcaccacc tggctgaaca gtttcctcat taaaagaaac
     3841 gtcaaacccg gccagagagt aagcattttt ggctacagaa tcttttgcgt tagcatgcca
     3901 gtagagccca tgagtgtcgc tgtgctgggc ggagttaggg gattgacctg cgttcccagc
     3961 acaccctacc agagacgcac ttgcaactaa agctgaaacc aaggatacaa accgtttatt
     4021 tttcatttat gacccttttt gaatgaattt tatgtctaga agcattattt catactcgcc
     4081 agtttctcaa acaacctaaa tgaagaaaaa atggaaaaat atgaagtgac caaaaatgag
     4141 tcttgtaaag aaaaatccgt caaatttaaa tcgtataata aaggggacta aatattcaga
     4201 ggcttttatg ggtaaaccaa cagatgagca aagggtcatc attgaaaatg caaatgctaa
     4261 taatatggtt attgcagcac cgggatctgg aaagtcattc acgatgatag aggcggtgat
     4321 ctctatcctc aggcagttcc cttatgccaa agtggggatg gtcacattta cccgtgcagc
     4381 aacaaattca ttggctgaaa aactgaaacg tcgattaagt aagaaagacc aggatagggt
     4441 gctggtcaat acattccacg gctttatccg gatgcaactg gatatggtca actggaaggg
     4501 caagatgttg attagctcgg cacagcgttc tgtaattcac agggcattga aggagtccgg
     4561 cgcgcctttt cgatacccgg atgcagaatt tgctattgac gcgattggaa gggaaatgga
     4621 tacggacatc atttcagttc gccatacgcg gcaacaaatc cacttgttca acacctatca
     4681 ggcaatatgt cagaaagacc acgttgcaga tttgaatgca ctctcacgtt ttgttgtggg
     4741 ccagatgtac tcggggaaaa tgcagccact taacttgaca catttagttg tagatgaagt
     4801 tcaggatacc gactcgattc agtatgcctg gatctcacta catacgaggg caggtgttaa
     4861 cactagcatc gttggggatg atgatcaggc aatttattca ttcagggcat caggtggagt
     4921 caaaatattc cagcagtttg aaaaacaatt caggcccaac atattttatt tgaatacttg
     4981 tttcagatgt gaaccagaga ttttgaaggt agcaggggca ttgattgaaa aaaatgttta
     5041 cagatacgct aaagatctcc gttcagctaa gggcggtggg ggaaaagttc atttccgctc
     5101 atatgttgat atggatgagc aaattcaagg cattctcaat ctgattaacc aagacccgat
     5161 tgggtgggca atcctcagca gaggtaatgc acatctcgat cagcttgaaa gcctgataga
     5221 gcagccggta cttcgttatg gtgggaagtc attttgggat gaaaaggaga caagcgatgt
     5281 attgcatttg atggcattct tccgacattc caacgatgtc cggttaatga agagggtgct
     5341 ggcgcttttc ggtgaaaatg aagaggtctt ggaccagaca gcattgagca tgaaaggaag
     5401 aaaagtaact tttggtgaac tgaacatccc gaatgaaagt tccctggaaa caagaacgct
     5461 tcattcaaac ttcacccggt ttacacagga gactcgggaa aaggttgaaa tagaaaaaag
     5521 gttcgcaaat ctgattaagt ggatggagtt gtcgtcgata aaaatgagga cgcagaaagg
     5581 atcaccatca ctgagcagaa ttgcgctgga tacctgtaag caatgggccg aaaagacagg
     5641 ctggcaaaat atgataaatc gagctgcggc gatgtgtctg gggccaaaga agaaggatga
     5701 ggaatataca ccggataaag tagttttatc gacgttgcac ggttcgaagg gtcttgaatg
     5761 gaaaaatgta attattatga gttgtaatgc agatcagatt ccaagtaagc ggtcagtggg
     5821 gcaggaggcg attgaagaag aaagacgact gctttttgtt gggtttacac gagcagagca
     5881 acagctgcat gtaatgtggt acggtgatcc gtctttcttt ttaagcgaat gtgctgaaga
     5941 taaattgaaa gaggctgcga aaagtagaac agaatccact ttgacagaat aattttgatt
     6001 tctttattta caggaatgaa cccgtgtagt ataacgtata tgagcttgaa tttattgctc
     6061 ccacatttca agtaacttta taaaagttac tttgcgcgtt aagcgcatat tcagccgtga
     6121 aaagcggcca gctttctaaa gctgcaaggc accaccggca aaatgtcacc tttgtcgctg
     6181 gtgcctttcc catgtctgaa aaaggacaat cgaatttacc aaatcgtaac taacttagct
     6241 cttaattgag ctattttcgt tgcgtacttc taatgccaga aagtataaat caggcggttc
     6301 atagcttacg ggagatgaat gacaggcttt gcctttgtgt tgggatattt aatgattcct
     6361 tccttcttac tatatatagg tgaccccaa
//
LOCUS       DAPHBJ010000227         6368 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.229, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000227 DAPHBJ010000000
VERSION     DAPHBJ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.229"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(283..1953)
                     /gene="betA"
                     /locus_tag="RHZ76_004056"
     CDS             complement(283..1953)
                     /gene="betA"
                     /locus_tag="RHZ76_004056"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HDV2814590.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDMGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     YAMTDHIIFDGKRAVGVEWQEGGSTIPTRATANKEVLLCAGAIASPQILQRSGVGSTE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPVLDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(1967..3439)
                     /gene="betB"
                     /locus_tag="RHZ76_004057"
     CDS             complement(1967..3439)
                     /gene="betB"
                     /locus_tag="RHZ76_004057"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HDV2814591.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWASMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGLDNGAWVAPTVFTDCSDEMTIVREEIFGPV
                     MSILTYESEEEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3453..4040)
                     /gene="betI"
                     /locus_tag="RHZ76_004058"
     CDS             complement(3453..4040)
                     /gene="betI"
                     /locus_tag="RHZ76_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HDV2814592.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKTRANSLTRHFITQHLPTD"
     gene            4169..6202
                     /gene="betT"
                     /locus_tag="RHZ76_004059"
     CDS             4169..6202
                     /gene="betT"
                     /locus_tag="RHZ76_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HDV2814593.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
BASE COUNT         1317 a         1763 c         1744 g         1544 t
ORIGIN      
        1 cacgaatctt tttcttcaga taaaataaaa tctcaatcag atcgatatcc tgtttgattt
       61 gttcacgcat aatatatcca gaaaataaaa tctgtcgcag ataaggttgt attaatagtc
      121 tgtatcagga atgttcgggg taaatatcag caaaaaacca gcatcatgaa tactggatat
      181 gaagcatgag agttacctca gtgtttatat aaggattcgg tccccctctc tggaacggta
      241 actctcaatc tgatcggttc ctgcgttagt tcacatcacg actcattttt tcgctctcac
      301 cggcatccca tttgccacaa aatatcccgc cgtgctcctc ggcagcgctt cctgtccacg
      361 aatcatatcc gctattttct cgccaatcat aattgtcgtg gcgttcaaat tcccggtgat
      421 aatctgcggc ataattgacg catccaccac gcgcaggcct tccagcccat gcacgcggcc
      481 ttcgccgtca accacggaca tctcgtcgta acccattttg caggtaccgc acggatggaa
      541 ggcggtttcg gcgtgattac gcacgaactc atcgagctgc tcatccgtct gacattccgt
      601 gccggggctg atttcgcggc cacgatactg atccagcacc ggttgatgca tgatctcccg
      661 ggtgatgcga attgcgtcgc ggaactcctg ccagtcctgc tcgtgtgaca tgtagttaaa
      721 cagaattgcc ggatgctggt gcgggtcgcg ggatttaatc cgcacatgcc cacggcttgg
      781 cgagcgcatt gagccgacgt ggcactggaa accgtgctct ttcactgcat tcgagccgtt
      841 atagttaatc gctactggca ggaaatggta ctgaatattc ggccacgcaa attcctctcg
      901 gctgcgaata aatccgcctg cttcaaagtg gttgctggca ccaacgccgg tgccgccaaa
      961 cagccactcc gcaccgattt tcggctgatt ccaccactgc agggcagggt agagggaaac
     1021 cggttctttg cactcatatt gcaggtacat ctccaggtga tcctgaagat tttcgccgac
     1081 gccgggtaaa tcatgcacca gcggaatatc aaactccgcc agcagttcag tgctacccac
     1141 gccggaacgt tgcaggatct gcggagaggc gatcgcacct gcacataaca gcacctcttt
     1201 attcgctgtc gcgcgggttg gaatggtgct gccgccttcc tgccactcga cgcctaccgc
     1261 gcgtttgccg tcaaaaatga tgtgatcggt catagcgtaa gtacgaatgg tcaggttcgg
     1321 gcgcgatttg gcctgatcga gatagccgcg cgcggtgctg gcgcgacggc cctgcggcgt
     1381 cacggtgcga tccatcgggc caaaaccttc ctgctgataa ccgttgagat cgtccgtgcg
     1441 cgggtagccc gcctgcacgc ccgcttcaat catcgcttca aacagcggat tgacgccggg
     1501 tttggaggta gtgacgctca ccgggccatc accgccgtga tagtcgtttt cacccatatc
     1561 gcgagtctcg gccttgcggt agtagggcag gcagtcgagg tagctccagt tctccagacc
     1621 gggttcttgc gcccagttat cgagatccag cgcattgcca cggatgtagc acatgccgtt
     1681 gatcagcgac gatccaccca gacctttacc gcgtccgcac tccatgcggc ggttattcat
     1741 aaacggttca ggttccgttt cataggccca gttgtagcgt ttaccctgta gcgggaatgc
     1801 cagggcagcg ggcatctggg tgcggaagtc aaagcgatag tccgggccgc ccgcttcaag
     1861 cagcagcacg gaggtattcg gatcttcagt cagacgggta gcgagaacgt tgccggctga
     1921 gccggcacca ataatgatgt agtcaaattg caaataaacc tcctggttag aatatggact
     1981 ggaatttagc catctcaacc tggatggact tcacctgggt gtaactctgg agcgtcatca
     2041 cgccgttctc gcggccaatg ccggagtgtt tgtagccgcc aacgggcatc tctgccgggg
     2101 attcgcccca ggtgttgatc cagcaaatac ccgcttccag ctgatgaatg acgcgatgcg
     2161 cgcggttcag gtccgccgtc acgattcccg ccgccaggcc gtagtcggta tcgttagcgc
     2221 ggcgaatgac ttcctcttcc gactcgtagg tcagaatgga catcaccggc ccgaagatct
     2281 cttcgcgcac aatggtcatt tcgtcgctgc aatcggtgaa cacagtcggc gcgacccatg
     2341 cgccgttatc gaggccatcg cctttcagta catcgccgcc gcacagtacg cgcgcgcctt
     2401 cctctttgcc tttggcgata tagcgcagca cgttatcgcg atgcgggaag ctgaccagcg
     2461 ggccgaagtt agtttgcgga tcgaaaacgt cgcccgcgcg aatgcgctca acgcgcgcca
     2521 gaattttctg ctcaaatgcg gctttgcatt tcgccggaac gaagacgcgg gtgccattgg
     2581 tacacacctg accggagctg aagaagttcg ccatcatggc gatatcggcg gcgagatcga
     2641 gatccgcatc gtcgaaaacg atcagcggcg atttaccgcc cagttccatg gtcacttctt
     2701 tcaaggaaga ggccgccgag ttagccatca cttttttgcc gctggcgaca ccgccggtaa
     2761 atgacacttt ggcaatgccc ggatgctcgg tcagatattg cccggtctcc gcgcccacgc
     2821 ccggcaacac gttaaatacg ccgtccggca ggcccgcttc gctgtaaatt tcagccagct
     2881 ttaacgcggt aagcggggtg acttcgctcg gtttgaaaat cattgcgttg cctgccgcca
     2941 gcgccggggc ggatttccac agggcaatct ggatcgggta attccacgcg ccaatccccg
     3001 ccaccacgcc cagcggttcg cggcgggtat aaacaaatga cgtctcacgc aacgggatct
     3061 ggctgccttc cagcgccggg atcagcccgg cgtagtactc cagcacgtcc gcaccggtaa
     3121 cgatatcgac ggttgaggtt tccgaatatg cttttccggt gtcgagggtt tccagttttg
     3181 cgagttcgtc attgcgttca cgcagaatat caacggcccg acgcagaata cgcgagcgct
     3241 ccatggcggt catcgacgcc cagatttttt gcccctgctg ggcgcttttc acggcgcgat
     3301 cgacatcctc gcgcccggcg gcctgcacgg tcgccagcac gttaccgttg gccgggttaa
     3361 tggtctcgaa ggtgcgaccg ctggtggcgg aggtataacc accatgtata taaagctgct
     3421 gttctgccat tcgggacatc acgtctcctc ggttaatcgg tgggtagatg ctgagtgata
     3481 aagtggcggg tcagggaatt agcgcgggtt ttatccagcg gtttgccgct cagagccgcg
     3541 cgcagccata atccatcaat cagcgcggcc aggccgtagc ccgcttcctg tgcctgttcg
     3601 cgcggcaatt cgcgacgaaa ctcgctcacc agattcgaca gcaagcggcg gctgctgacc
     3661 tgctgtaaac gatagagcat cggctgatgc atactgctgg cccagaacgc cagccaggct
     3721 ttcatcgccg cactgctcac ctgcgtttca tcgaagtttc cgccaacaat cgcctgtaat
     3781 cgctgctctg cactgccctg cggaagtgca tgtaatcgat tcaaaaccgc gtcacgcagc
     3841 tgactggtga tatcgcgcat ggttgcttcc agcagaccat ttttgtccct gaaatagtgg
     3901 ctgatgatcc ccgtagaaac gcctgcacgg cgggcgatct gcgcgatcgt tgcatcgtgc
     3961 atgcccactt catttattgc ttccagtgtg gcgtcgatca gttgtctgcg ccggatcgac
     4021 tgcatcccca atttgggcat tttcgccact ccattcatca gcggtgttta tctattaaag
     4081 cggttattga ttggacgttc aatataaaat gtgtcttaat tgttacgaat ttgattttaa
     4141 atagtaacaa taacagtggg gatactggat gacagacctt tcacacagca gggaaaagga
     4201 caaaatcaat ccggtggtgt tttacacctc cgccggactg attttgttgt tttccctgac
     4261 aacgatcctg tttcgcgact tctcggccct gtggattggc cgcacgctgg actgggtttc
     4321 taaaaccttc ggttggtact atctgctggc ggcaacgctc tatattgtct ttgtggtctg
     4381 tatcgcttgt tcgcgttttg gttcggtgaa gctcgggcca gaacaatcca aaccggaatt
     4441 cagcctgctg agttgggcgg cgatgctgtt tgctgccggg atcggtatcg acctgatgtt
     4501 cttctccgta gccgaaccgg taacgcagta tatgcagccg ccggaaggcg cgggacagac
     4561 gattgaggcc gcgcgtcagg cgatggtctg gacgctgttt cactacggct taaccggctg
     4621 gtcgatgtat gcgctgatgg gcatggcgct cggatacttt agctatcgtt ataatttgcc
     4681 gctcaccatc cgctcggcgc tgtacccgat cttcggtaaa cggattaacg ggccgatagg
     4741 tcactcagtg gatattgcag cggtgatcgg cactatcttc ggtattgcca ctacgctcgg
     4801 tatcggtgtg gtgcagctta actatggctt gagcgtactg tttgatattc ccgattcgat
     4861 ggcggcaaaa gcggcactga tcgccttgtc ggtgatcatc gccactatct cggtgacatc
     4921 cggtgtcgat aagggcattc gcgtgttatc ggagcttaat gtcgcgctgg cgctgggatt
     4981 gatcctgttc gtgttgttta tgggcgacac ttcgttcctg cttaatgcac tggtgctgaa
     5041 tgttggcgac tatgtgaatc gctttatggg catgacgctc aacagttttg ccttcgaccg
     5101 tccagttgag tggatgaata actggacgct cttcttctgg gcatggtggg tggcatggtc
     5161 gccgtttgtc ggcttgttcc tggcgcgtat ctcgcgtggg cgtaccattc gccagttcgt
     5221 gctgggcacg ttgattattc cgtttacctt cacgctgtta tggctctcgg tgttcggcaa
     5281 tagcgcgctg tatgaaatca tccacggcgg agcggcattt gccgaggaag cgatggtcca
     5341 tccggagcgc ggcttctaca gcctgctggc gcagtatccg gcgtttacct ttagcgcctc
     5401 cgtcgccacc attactggcc tgctgtttta tgtgacctcg gcggactccg gggcgctggt
     5461 gctggggaat ttcacctcgc agcttaaaga tatcaacagc gacgcccccg gctggctgcg
     5521 cgtcttctgg tcggtggcga ttggcctact gacgctcggc atgctgatga ccaacggtat
     5581 atccgcgctg caaaacacca cggtgattat ggggctgccg ttcagctttg tgatcttctt
     5641 cgtgatggcg gggttgtata aatctctgaa ggtagaagat taccgccgtg aaagtgccaa
     5701 ccgcgatacc gcaccgcgac cgctggggct tcaggatcgc ctgagctgga aaaaacgtct
     5761 ctcgcgcctg atgaattatc cgggcacgcg ttacactaaa cagatgatgg agacggtctg
     5821 ttacccggca atggaagaag tggcgcagga gctgcggttg cgcggcgcat acgtggagct
     5881 aaaaagcctg ccgccggaag agggacaaca gttggggcat ctggatttgt tggtgcatat
     5941 gggcgaagaa caaaactttg tctatcagat ttggccgcag caatactcgg tgccgggctt
     6001 tacctaccgc gcacgtagcg gtaaatcgac ctactaccgg ctggaaacct tcctgttaga
     6061 aggcagccag ggcaacgacc tgatggacta cagcaaagag caggtgatca ccgatattct
     6121 tgaccagtac gagcggcacc ttaactttat tcatctccat cgtgaagcgc cgggccatag
     6181 cgtgatgttc ccggacgcgt gattgttatt gcataaaaca gcgccatgtc tgcatatggc
     6241 gctgtttctc ctacctcttg atacgttata tctatacggt taagccctta gtatctattg
     6301 atgattacca gacaacagat aataaaaaag aaaagaacta ttgcagccca aaacctacat
     6361 ttgggctg
//
LOCUS       DAPHBJ010000228         6316 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.230, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000228 DAPHBJ010000000
VERSION     DAPHBJ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.230"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            108..1037
                     /gene="pfkB"
                     /locus_tag="RHZ76_004060"
     CDS             108..1037
                     /gene="pfkB"
                     /locus_tag="RHZ76_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HDV2814594.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNENEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSTLVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            1138..1428
                     /gene="ghoS"
                     /locus_tag="RHZ76_004061"
     CDS             1138..1428
                     /gene="ghoS"
                     /locus_tag="RHZ76_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDV2814595.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            1534..2394
                     /locus_tag="RHZ76_004062"
     CDS             1534..2394
                     /locus_tag="RHZ76_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDV2814596.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSSNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(2435..2971)
                     /locus_tag="RHZ76_004063"
     CDS             complement(2435..2971)
                     /locus_tag="RHZ76_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HDV2814597.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            3118..3786
                     /gene="hxpB"
                     /locus_tag="RHZ76_004064"
     CDS             3118..3786
                     /gene="hxpB"
                     /locus_tag="RHZ76_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HDV2814598.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLADVKLSSLTELTAK
                     DLLG"
     gene            3949..4539
                     /locus_tag="RHZ76_004065"
     CDS             3949..4539
                     /locus_tag="RHZ76_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV2814599.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            4672..6063
                     /gene="tcyP"
                     /locus_tag="RHZ76_004066"
     CDS             4672..6063
                     /gene="tcyP"
                     /locus_tag="RHZ76_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HDV2814600.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHH"
     gene            complement(6088..>6316)
                     /locus_tag="RHZ76_004067"
     CDS             complement(6088..>6316)
                     /locus_tag="RHZ76_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814601.1"
                     /translation="PGMGSGILEDIMSIPDSSLRKRLGYEVLSFSLQAHSLSQECIDK
                     LDIFFADDLFKYESVCIAAMEHLKSKATAPI"
BASE COUNT         1557 a         1556 c         1568 g         1635 t
ORIGIN      
        1 atatttatct acaaaatttt aaataaagct ccaataaatc atattgttaa tttcttcact
       61 ttccgctgat tcggtgccag actgaaatca gcctatagga ggaaatgatg gtacgtatct
      121 atacgttgac acttgcgccc tctctcgata gcgcaacaat taccccgcaa atttatcccg
      181 aaggaaaact gcgctgtacc gcaccggtgt tcgaacccgg gggcggcggc atcaacgtcg
      241 cccgcgccat tgcccatctt ggaggcagtg ccacagcgat cttcccggcg ggtggcgcga
      301 ccggcgaaca cctggtttca ctgttggcgg atgaaaatgt ccccgttgct actgtagaag
      361 ccaaagactg gacccggcag aatttacacg tacatgtgga agcaagcggt gagcagtatc
      421 gttttgttat gccaggcgcg gcattaaatg aaaatgagtt tcgccagctt gaagagcaag
      481 ttctggaaat tgaatccggg gccatcctgg tcataagcgg aagcctgccg ccaggtgtga
      541 agctggaaaa attaacccaa ctgatttccg ctgcgcaaaa acaagggatc cgctgcatca
      601 tcgacagttc tggcgaagca ttaagtgcag cactggcgat tggtaacatc gagttggtta
      661 agcctaacca aaaagaactc agtacgctgg tgaatcgcga actcacccag ccagacgatg
      721 tccgcaaagc cgcgcaggaa atcgttaata gcggcaaggc caaacgggtt gtcgtttccc
      781 ttggtccaca aggagcgctg ggtgttgata gtgaaaactg cattcaggtg gtgccaccac
      841 cggtgaaaag ccagagtacc gttggcgctg gtgacagcat ggtcggcgcg atgacactga
      901 aactggcaga aaatgcctct cttgaagaga tggttcgttt tggcgtagct gcggggagtg
      961 cagccacact caatcagggt acacgtctgt gctcccatga cgatacgcaa aaaatttacg
     1021 cttacctttc ccgctaacaa aaacattccc ccagcattgg gggaatcatc accaacctgt
     1081 cggcaacgcg tttctccgac tatgctcaaa agtcatgtga taacaaaggg gtgaactatg
     1141 gccagtggcg atcttgtccg ttatgtcata accgtcatgt tgcatgagga tacattgact
     1201 gaaattaacg agttgaataa ttacctgact cgcgacggtt ttttgctcac catgacggat
     1261 gatgagggaa atatccatga gctggggact aacacttttg gacttatcag tacccaaagt
     1321 gaagaagaaa ttagagaact ggtttcgggg cttacccaaa gtgcaaccgg caaagatcct
     1381 gaaatcacca tcacgacctg ggaggaatgg aatagtaaca gaaaataaat ggtttttggg
     1441 caataatcag tctgtggtgt gcgttagctc gtgtttttac accgcattct tgcgctaacc
     1501 ttatgatctg gcagacaaca tgggagagac atcatgtggc aggcaatcag tcgtcttttg
     1561 agcgagcagt taggtgaagg cgaaatcgaa ctgcgtaatg aactgcctgg cggagaagtc
     1621 catgccgcat ggcatttgcg ctatgcagga catgactttt tcgtcaaatg tgatgaaagg
     1681 gaactgcttc ccggttttac cgccgaagcc gaccaactgg agttactgtc gcgtagtaaa
     1741 accgtcaccg tgcctaaggt ttgggcagtt ggcgctgacc gtgactacag ttttctggtg
     1801 atggattatc tcccacctcg tccgctggat gcgcatagcg catttattct tggtcagcaa
     1861 attgcgcgtt tacatcaatg gagtgaccaa ccacaatttg gcctcgattt tgataactcg
     1921 ctctccacaa ctccacagcc caacacctgg caacgtcgct ggtcaacgtt ttttgctgaa
     1981 caacggattg gctggcagtt ggaactggca gcagagaaag ggatcgcttt cggcaatatc
     2041 gacgccatcg tcgagcatat tcagcagcgt ctggcctcgc atcaaccgca gccttctctg
     2101 ttgcacggcg atttatggtc cagcaactgt gcgctgggtc cggatggccc gtacattttc
     2161 gaccctgcct gctactgggg tgaccgagag tgcgacctgg cgatgttacc gctgcatact
     2221 gaacagccgc cacaaatcta tgacggctat cagtcagtat ccccgctacc tgccgatttc
     2281 cttgaacgtc aaccggttta ccaactctac acgctgttaa atcgtgcaag attatttggc
     2341 ggtcagcatt tggttattgc tcagcagtca ttggatagat tattagcagc atgatatggg
     2401 ttgaggataa tggccgctcc gtgcggcctt ttgattaaat aaacccaagc agagaaaaga
     2461 atacataacc tgcagcaata caaataaccg gcaggatata cagtgagaaa aactgcagga
     2521 aaatagtatg gtgcggaaca acaatacggg actcaatttg ttcacgcgtt agcccctctt
     2581 cccctttggc tttttccaga atgagttgat cttcaacacc ttcccgcaag aagcgcgcct
     2641 ggcgactcat ccgggcaccg gaatcctgca acgccagtcc gacaaaaatc aggatgaaaa
     2701 tcacccagaa cataacgttc acgccaccat taaaattggg cgtcggggag ttataccaga
     2761 atagattcaa aaaaggcgta ttcgcctgca tcatatcgat catgacatga gtaaagtcga
     2821 gcatgaccgc attaatgcct tcctggtttt cctgtcgggt attcataaat ttcagcaaag
     2881 aaatcaatgt agagatcaat gcaggtataa aaatcaccca acccaaaatc cttttcaaaa
     2941 cagcaatgcg tccagcttgt tgatacgtca tgagttctcc ttgattaaga cgcgtcgttt
     3001 cacttagttt acctgtagat atctgttttc gcccattctt taaaggcgat atgataggcg
     3061 cttaatcata agcacggctt aataccttac acataatgct ctaaaggaga ggttgtaatg
     3121 tcaaccccgc gtcagattct tgctgcaatt tttgatatgg atggattact tatcgactca
     3181 gaacctttat gggatcgagc cgaactggat gtgatggcaa gcctgggggt ggatatctcc
     3241 cgtcgtaacg agctgccgga caccttaggt ttacgcatcg atatggtggt cgatctttgg
     3301 tacgcccggc aaccgtggaa tgggccaagc cgtcaggaag tagtagaacg ggttattgcc
     3361 cgtgccattt cactggttga agagacacgt ccattattac caggcgtgcg cgaagccgtt
     3421 gcgttatgca aagaacaagg tttattggtg gggctggcct ccgcgtcacc actacatatg
     3481 ctggaaaaag tgttgaccat gtttgactta cgcgacagtt tcgatgccct cgcctcggct
     3541 gaaaaactgc cttacagcaa gccgcatccg caagtatatc tcgactgcgc agcaaaactg
     3601 ggcgttgacc cactgacctg cgtagcgctg gaagactcgg taaatggcat gatcgcctct
     3661 aaagcagccc gcatgcgttc catcgtcgtt cctgcgccag aagcgcaaaa tgatccacgt
     3721 tttgtattag cagacgtcaa actctcatcg ctgacagaac tcaccgcaaa agaccttctc
     3781 ggttaatgac caggggcagt gatctcgctg cccctggttc tttatctgaa tttgcattca
     3841 actgacggat taatcgtcaa tttaagagaa agagttacac cgtcaccact tccgtgcact
     3901 gtataaaaat cctatactgt acgtatcgac agtttagtga gttttatcat gacggcggaa
     3961 ggtcaccttc tcttttctat tgcttgtgcg gtatttgcca aaaatgccga gctgacgccc
     4021 gtgctggcac agggtgactg gtggcatatt gtcccttccg caatcctgac gtgtttgtta
     4081 ccggacatcg atcacccaaa gtcgtttctt gggcaacgat taaagtggat atcaaaaccg
     4141 atcgcccgcg cttttgggca tcgtggtttt acccacagtc tgctggcggt atttgcgctg
     4201 ctggcaacct tttaccttaa ggttcctgaa agctggttca ttccggctga tgcgctacaa
     4261 ggaatggtgc tgggttattt aagccacata cttgccgata tgctgacacc cgccggtgtt
     4321 cccctgctct ggccatgccg ctggcgtttc cgcttaccta tcctggttcc ccaaaagggc
     4381 aaccaactgg aacgttttat ctgcatggca ttatttgtct ggtcggtatg gatgccccat
     4441 tcattacccg aaaacagcgc tgttcgttgg tcatcgcaaa tgattaatac cttgcagatc
     4501 cagtttcatc ggcttattaa gcatcaggtt gaatactaaa aaggcaaaaa tcacctttct
     4561 gtaataagca attccatttg aatataagag ccagctcaca gttctgttaa tcttgcgcca
     4621 acactatgac tgctacgcag tgatagaaat aataagatca ggagaacggg gatgaacttt
     4681 ccattaattg cgaacatcgt ggtgttcgtt gtactgctgt ttgcgctggc tcagacccgc
     4741 cataaacagt ggagtctggc gaaaaaagtg ctggtgggtc tggtgatggg tgtggttttt
     4801 ggccttgccc tgcataccat ttatggttct gacagccagg tacttaaaga ttctgtacag
     4861 tggtttaaca tcgttggtaa cggctatgtt caactgctgc aaatgatcgt tatgccgtta
     4921 gtcttcgcct ctattctgag cgcggttgcc cgtctgcata acgcatctca gttaggcaaa
     4981 atcagttttc tgaccatcgg tacgcttttg tttaccacgc tgattgcggc gctggtcggt
     5041 gtgctggtca ccaacctgtt tggtttgacg gctgaaggtc tggttcaggg tggtgcagaa
     5101 actgcacgtc tgaacgccat tgaaagtaac tatgttggta aagtctctga cctgagcgtt
     5161 ccgcagctgg tcttgtcctt tatcccgaaa aacccgtttg ccgatctgac cggagccaat
     5221 ccgacgtcaa ttatcagcgt ggtaattttt gccgcattcc tcggtgtagc tgcgctgaaa
     5281 ctgctgaagg atgatgcgcc gaaaggtgaa cgcgtcttaa ccgctatcga taccctgcaa
     5341 agctgggtga tgaaactggt tcgcctggtc atgcagttga ccccttacgg cgttctggca
     5401 ctaatgacca aagtggttgc aggttctaac ctgcaagaca tcatcaaact gggaagtttc
     5461 gttgtcgcgt cctacctcgg tctgctgatt atgtttgcag tgcatggcat tctgctgggc
     5521 attaatggcg tgagtccgct gaagtacttc cgtaaggtat ggcctgtgct gacgtttgcc
     5581 tttaccagcc gttccagtgc tgcgtctatc ccactgaatg tggaagcaca aacgcgtcgt
     5641 ctgggcgttc ctgaatccat cgccagtttc gccgcctctt tcggtgcgac cattggtcag
     5701 aacggctgcg ccggtttgta tccggcaatg ctggcggtga tggttgcgcc tacggttggc
     5761 attaacccgc tggacccgat gtggattgcg acgctggtcg gtattgttac cgttagctcc
     5821 gcaggcgttg ccggtgtcgg tggtggtgca actttcgccg cactgattgt gctgcctgcg
     5881 atgggcctgc cagtaaccct ggtggcgctg ttaatctccg ttgaaccgct tatcgacatg
     5941 ggccgtacgg cgctaaacgt tagtggctcg atgacagctg gcacgctgac cagccagtgg
     6001 ctgaagcaaa ccgataaagc cattctggat agcgaagacg acgccgaact ggcacaccat
     6061 taattcttat gctggcaacg gtccgtttca tataggggcc gttgccttac tttttaaatg
     6121 ttccatcgcg gcgatgcata ctgactcata tttaaacaga tcgtcagcga aaaaaatgtc
     6181 caacttatca atacattcct ggctcaatga atgtgcctgt agcgagaagc ttaaaacctc
     6241 atacccgaga cgttttctaa gtgaactatc tggaatactc ataatatctt caagtatacc
     6301 acttcccatt cccgga
//
LOCUS       DAPHBJ010000229         6309 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.231, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000229 DAPHBJ010000000
VERSION     DAPHBJ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.231"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            352..1902
                     /locus_tag="RHZ76_004068"
                     /pseudo
     CDS             352..1902
                     /locus_tag="RHZ76_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001568839.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2154..2483)
                     /locus_tag="RHZ76_004069"
                     /pseudo
     CDS             complement(2154..2483)
                     /locus_tag="RHZ76_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
     gene            2601..3326
                     /locus_tag="RHZ76_004070"
     CDS             2601..3326
                     /locus_tag="RHZ76_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDV2814602.1"
                     /translation="MAYSIGEFARLSGITATTLRAWQRRYGLLKPQRTEGGHRQYSDE
                     DVQQALKILDWVKKGVPIGQVKPLLERPTSRLTNNWLTLQQSMLQRLQDGKIESLRQM
                     IYDAGREYPRPELVANVLRPLRSQVSANVAAAMTLREILDGIIIAYTSFCLEGDKKAP
                     GDNILLSGWNLNDPCEVWLEALTRTGQGHRIDILPTPPATLAPEIFPDRHWLLVTRGK
                     LTAARKKQIEQWQQQVSLEVIIL"
     gene            3426..3836
                     /gene="uraH"
                     /locus_tag="RHZ76_004071"
     CDS             3426..3836
                     /gene="uraH"
                     /locus_tag="RHZ76_004071"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HDV2814603.1"
                     /translation="MKKTASLLMLTTLAFAPAAFSAPAGTLSVHILDQQTGMPPSDVT
                     VTLEKQQQDKWTPIASGKTDHDGRIKSLYPEDQDMQPGVYKVAFKTADYFHGKKLDSF
                     FPEIPVLFTVTRTNEKLHIPLLLSQYGYSTYKGS"
     gene            3939..4898
                     /locus_tag="RHZ76_004072"
     CDS             3939..4898
                     /locus_tag="RHZ76_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HDV2814604.1"
                     /translation="MTTKPVLGISGCLTGSAVRFDGGHKRMGFVMDELAQWVSFRPVC
                     PEMAIGLPTPRPAIRLTLTDSGETQLRFSKPPHDDITQKMADFTADYLPKIGDLSGFI
                     VCAKSPSCGMERVRLYDENGNRGRKEGVGLFTAALLETYPWLPVEEDGRLHDPVLREN
                     FIERVFALHELNTLRAKGLTRRALLDFHSRYKLQLLAHHQAGYREIGPFVASLHEWED
                     LDAFFVAYREKLMTILKKPASRKNHTNVLMHIQGYFRNQLNSRQRVELRDVILHYRDG
                     LLPILAPLTLLKHYMAEYPDRYLMTQNYFDPYPDDLGLRLAVT"
     gene            complement(5042..6262)
                     /locus_tag="RHZ76_004073"
     CDS             complement(5042..6262)
                     /locus_tag="RHZ76_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISKox3 family transposase"
                     /protein_id="HDV2814605.1"
                     /translation="MDEKSLYAHILNLSDPWQVKSLSLDENAGSVTVTIEIAENTRLA
                     CPTCGKSCSVHDHRHRKWRHLDTCQFTTIVEADVPRIMCPEHGCLTLPVPWAGPGSRY
                     TLLFESFVLSWLKISTVDAVRKQLKLSWNAVDGIMTRAVKRGLARIKKPLSARHMNVD
                     EVAFKKGHRYITVISDRDGRALALTDDRGTESLAGYLRTLTDGQLLAIKTLSMDMNAG
                     YIRAARIHLPSAVEKIAFDRFHVAKQLGEVVDKTRQNEHPHLPVESRHQAKGTRFLWQ
                     YSDKWMTESRQEKLMWLRAQMKLTSQCWALKELAKDIWNRPWSEERRSDWQRWLALAA
                     NSDVPMMKNAAKTIGKRLYGILNAMRHSVSNGNAEALNSKIRLLRIKARGYRNRERFK
                     LGVMFHYGKLNMAF"
BASE COUNT         1602 a         1637 c         1553 g         1517 t
ORIGIN      
        1 gaagagcgtc ttgttgccta caatgctgtt ggacaactgg agtggaatgc cggttcctgg
       61 cgttttggtg agaaaggtgt tgcatggctt aaagaaacaa aagatcgcgg ctttaagtgg
      121 gacgaagttt ctagtcgtat taaggggctc tcacaaatga atatttcttc ggaatttcag
      181 gattttatgc gagcttatca catgcatctg gtgtgtatca ttggcggatt accatcaggt
      241 tctacactag acaaaccatt acaggtcatg aagcgctggt attgggaaat ggtaaataaa
      301 acaggtcaaa cgcatccaat gtatctgact tccgatatta ttcatgcagc gatggagcgg
      361 catcatgaga attctgacag tcctgataat gtcagtgact attgcgatat tgcagtaaag
      421 gccattgctt tactacgtca atatgatctt ttcttagtta atattgaagt caaaaataag
      481 tatccttatc gaaatggttc taacagcact aaagaaagga agaaagcgca agaagccacc
      541 cctgatcaaa ctgatgatga aagactcata tcaatccgtg catttatgtg tgttattgag
      601 ttgatcgctt tagctgagaa tagttatcag cgtatctttt acaatatgtt gttactcaca
      661 attatttgcg gtttccgttt ccaggaaata atgttgctga aaatgacttc tttggttaag
      721 cgagaaatta cggataaaga taaacgtcag cacgcaatag atcaagactg gcctacttac
      781 aggttgggta ttgagtatct tggtgcaaaa aaggctggat ggcggatcca ttggctggcg
      841 ccaagcactt attctgtcgt tgagatgatt tataaatctg ttcgagaatt gacctctggg
      901 ttccgtaaaa cgattcaggg cttcagagat tctaatttca ctaatatgct cccaggtaag
      961 atcgctgatt ttcctgatga atggattgag caaaattatc ttgaagacat catgtttagt
     1021 ggttctggtg gctccaggaa tgatcttgga aagagtattc ggaattccat tgcgaacttt
     1081 agcggacata aacctaaggt aatacccgtt cataaacaga agaacagctg gtacttcact
     1141 aaagaacagt tgaatgatta tgtctatcag cgttacgctc aagcaaaaaa ctttgagcct
     1201 gggcaccagt gcataatttc agccaaaaac aatggtgact gggaacactt caattatgaa
     1261 gacctgatgt tcatcatgcc ggaaggtgca tttggaattg gtaaagattt tgtttcattg
     1321 caacatattt ttccacttga tgaaaactca atagaagctt ggttaggcgg aacacaaaac
     1381 cgaaaatcta tctttgatca ctttaatctt tttgaagatg atgggtcccg tgtcattatc
     1441 aaaaagcatg taccgagaca taacataaat acattcttgg caatcgctgg agtgacggat
     1501 catattcagg ccatcctaat ggggcgtgtt gatatcaccc agaataaaca ctatcagcat
     1561 caggccgaat cacagtccta tcagaccgct tctctagcaa tcaatatgct ggaaaaggct
     1621 tatgatgaga acaatcaagc tctcacgaag agtttccagc tatcactctt tactacgcag
     1681 cagaagccct gcattccggt caacctaccc tcccccagaa ctgaggttag ccgcagaatg
     1741 gcagctctat cacggtcttc caagacggta aaaaacacag gtgttggaaa cgttaaatta
     1801 acaacctcac taatgataaa tcctgagctc aataccgagc ataatctgaa gcagaacatg
     1861 cagacttttg gtgaaacaac tgctgaggtt gtggactata tggggtcagt ttggagatcg
     1921 gaaatttttg tacgttaagc gcattattta accaatcttg gttcgtaagg ggttgatttt
     1981 gcgtcagtgg ctatcgcttt taagttgtcg ctcttgcagt atcgcattta ggttatccga
     2041 ttaaggtcaa acctctgaaa atgccgtata gcgcgggagt acaccctatt gctacaggta
     2101 agcctgttca aaaaacaggc ttaccgtaca ataattctct atatccaaac tgaccccaag
     2161 aaggacacct tcagcgcggc cgtggtcggt tcgccggtaa agtaggcttt accctcgctc
     2221 tcattccact tgttcttcac agggtcgtag ccgcggttaa gcacgctgat gccgccgtcg
     2281 ctgcgcttgc cgtaggtggc ggtaacctgc tccaggccgc gttcaaagcg gttttccagg
     2341 cgagcgactt cgtaccattt gccaagatag cggctggcgt caaaaccgga aatgggctgc
     2401 acgcccttcg gtggggtagg ggatttacag gctaccagcg tcagggcaat ggcaacaccg
     2461 gtcacaacag gccatagctt catgagaatt cctttcattt gcacggttgg aaattaagtg
     2521 tagtgcaccg cggcggcgct tcatcccatc cgcgaaaatt cgtagtatat tgagagtatt
     2581 cctcaactct ggacacggac atggcttact ccatcggcga attcgccaga ctcagcggga
     2641 tcaccgccac cacgctgcgg gcctggcagc ggcgctacgg cttactcaag cctcagcgca
     2701 cggagggcgg ccaccgccag tacagcgatg aggacgtcca gcaggcgcta aaaatcctcg
     2761 actgggtaaa aaaaggcgtc ccgattggcc aggtcaaacc gctgctggag cgccctacgt
     2821 cgcgcctgac caacaactgg ctcacgctgc agcagagcat gttacagcgc ctgcaggacg
     2881 gcaaaatcga gtccctgcgc cagatgattt acgacgcagg acgcgagtac ccaagaccag
     2941 aactggtcgc gaacgtgctg cgtccgctgc gcagtcaggt gtcggcaaac gtcgccgccg
     3001 ccatgaccct gcgggagatc ctcgacggca tcatcatcgc ctacacctcg ttttgcttgg
     3061 aaggcgataa aaaagcgccg ggcgacaaca tcctgctcag cggctggaac ctgaatgacc
     3121 cgtgcgaagt ctggctcgaa gccttaaccc gcaccgggca aggccaccgt atcgatattt
     3181 tacccacgcc gcccgccacg ctggccccgg agattttccc ggaccgccac tggcttctgg
     3241 tcacgcgcgg taaactcacc gccgcgcgca aaaagcagat cgagcagtgg cagcagcagg
     3301 tatcgcttga agtgattatc ctctaatcat tttctcctgc gggaatcttc acgaaaagtt
     3361 gaatattttc ccgcgccaca aatgctaacg ttttcctgtg accgacaacc ccacaggaaa
     3421 acacaatgaa aaaaactgcc tctctgctga tgctgaccac cctggccttt gcccctgccg
     3481 cctttagcgc ccctgccggc acgctgagcg tacacattct tgaccagcaa accgggatgc
     3541 cgccatcgga cgttaccgtc acgctggaaa aacagcagca ggacaaatgg accccaattg
     3601 ccagcggcaa aactgaccat gacggccgca tcaaatcgct ttatccggag gatcaggata
     3661 tgcagcccgg cgtctataaa gtggccttta aaaccgcaga ttacttccac gggaagaagc
     3721 tggactcctt cttcccggag atcccggtgc tgttcaccgt aacccggacc aacgaaaagc
     3781 tgcacatccc tctcctgctg agccaatacg gttattcaac ttacaagggc agttaagaaa
     3841 cgttgatttt aaaggcaaat aaaaaataat tccgctaata cttggacaaa ttagtcatgt
     3901 tgtgtaagtt ttaaagagaa gaacgggagg accacaccat gacaacaaaa cctgtgctcg
     3961 gtattagcgg atgtttgacc gggtctgccg ttcgcttcga cggcggacac aagcgtatgg
     4021 gctttgtcat ggatgaactg gcccagtggg tgagtttcag gcccgtctgc ccggaaatgg
     4081 cgattggcct gccgacgcca cgcccggcca tacgcctgac gctgacggac agcggcgaaa
     4141 cgcagctccg cttcagcaag ccgccccatg atgatataac gcagaaaatg gccgacttta
     4201 cggcggacta tctgccaaaa atcggcgatc tgtcgggatt tatcgtctgc gcgaaatcgc
     4261 caagctgcgg catggaacgc gtgcggctat atgatgaaaa cggcaaccgg ggccgcaagg
     4321 agggtgtcgg gctgttcacc gccgccctgc ttgaaaccta cccgtggctg cccgtggagg
     4381 aagacggtcg gctgcacgac ccggtgctgc gggaaaactt tatcgagcgg gtatttgccc
     4441 tgcacgagct gaacacgctg cgcgctaaag gcctgacgcg ccgcgcgctg ctggatttcc
     4501 acagccgcta taaattgcag cttctggcgc accatcaggc gggctaccgc gagattggcc
     4561 cgttcgttgc gtcgctgcat gaatgggaag atctggacgc gttcttcgtg gcgtaccgcg
     4621 aaaagctgat gaccatcctg aaaaagcccg cctcgcggaa aaaccatacc aacgtgctaa
     4681 tgcatatcca gggctatttc cgtaaccagc tgaacagccg ccagcgcgtc gagctgcgcg
     4741 atgtgatcct gcactatcgc gacgggctgc tacccatcct cgcgccgttg acgctactga
     4801 aacactacat ggccgaatac cctgaccggt atctgatgac gcaaaactac tttgatccct
     4861 atcctgatga tttgggtctg cgtctggcag taacctgacg ataaaaatgc gaaggcccgt
     4921 ttgacccaca gaggcccaaa gcagtacccc gtttagacga caccctcact gtcgtctctt
     4981 ttttgcattc cacacaaaag actgtaatta tgtgggtctt ccccgatcat ggtgggaagg
     5041 ctcagaacgc catattcagc tttccgtagt ggaacatcac ccccagttta aagcgctccc
     5101 ggtttcggta tcccctggct tttatcctca gcagcctgat cttgctgtta agtgcctccg
     5161 catttccgtt tgagacactg tgtcgcatcg cattcaggat cccgtacagc ctttttccta
     5221 tcgttttcgc ggcatttttc atcatgggaa cgtcactgtt agccgccagc gccaaccatc
     5281 tctgccagtc actccgtctt tcctcgctcc atggcctgtt ccagatatcc tttgccagct
     5341 ctttcagcgc ccagcactgg ctcgtcagct tcatctgtgc acgcagccac atcagctttt
     5401 cctgccggga ttcggtcatc cacttatcgc tgtactgcca caggaagcgg gttccttttg
     5461 cctggtgtcg gctttcaaca gggaggtgcg gatgttcatt ctgacgggtt ttatcaacta
     5521 cctcgcccag ttgcttcgcc acatggaagc ggtcaaaggc gattttctca accgcactgg
     5581 gtaagtggat acgcgctgct cttatatagc ccgcgttcat gtccattgag agcgttttga
     5641 tagccagcaa ctgcccatca gtgagcgtgc gaagatagcc ggcaagactc tctgtgccgc
     5701 gatcatccgt taaggccagc gcccgaccat cgcgatcgga gatcaccgtt atgtaacgat
     5761 gtcctttttt aaaggcgacc tcatccacat tcatatgacg ggcggataat ggctttttta
     5821 tccgggcaag acctcgctta actgcccggg tcataatgcc gtcaaccgca ttccaactga
     5881 gcttaagttg cttcctgaca gcatcaacgg tgctgatttt cagccatgag agaacgaacg
     5941 attcgaatag caacgtatac cggcttccgg ggccagccca cggaacaggc aacgtcaggc
     6001 agccatgctc cggacacata attcgtggaa catcggcttc aacaatagtg gtgaactggc
     6061 aggtatcaag atggcgccat ttacgatgac ggtgatcgtg aacagaacag gatttaccgc
     6121 aggtcggaca ggctagccgg gtgttttcag cgatctcaat agtgacagta acagaaccgg
     6181 cattttcatc gagagaaagg gactttacct gccacggatc ggacaggttg agaatatgag
     6241 cgtagaggga cttttcgtcc atggcggtga cctctggcga ttaaatacac cattatcatg
     6301 ccttcagcc
//
LOCUS       DAPHBJ010000230         6246 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.232, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000230 DAPHBJ010000000
VERSION     DAPHBJ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.232"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            84..974
                     /gene="alsR"
                     /locus_tag="RHZ76_004074"
                     /gene_synonym="rpiR"
     CDS             84..974
                     /gene="alsR"
                     /locus_tag="RHZ76_004074"
                     /gene_synonym="rpiR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="als operon DNA-binding transcriptional repressor
                     AlsR"
                     /protein_id="HDV2814606.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            1033..1968
                     /gene="alsB"
                     /locus_tag="RHZ76_004075"
     CDS             1033..1968
                     /gene="alsB"
                     /locus_tag="RHZ76_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HDV2814607.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLCNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEAFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            2095..3627
                     /gene="alsA"
                     /locus_tag="RHZ76_004076"
     CDS             2095..3627
                     /gene="alsA"
                     /locus_tag="RHZ76_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HDV2814608.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNISYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGIVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHETVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNMAISRSLKDGGYKGAMGLFHEVDEQRTAENQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCCPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     QE"
     gene            3606..4586
                     /gene="alsC"
                     /locus_tag="RHZ76_004077"
     CDS             3606..4586
                     /gene="alsC"
                     /locus_tag="RHZ76_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HDV2814609.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGV
                     DPFLAAMIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            4597..5292
                     /gene="alsE"
                     /locus_tag="RHZ76_004078"
     CDS             4597..5292
                     /gene="alsE"
                     /locus_tag="RHZ76_004078"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HDV2814610.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLATKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHDMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            5276..6205
                     /gene="alsK"
                     /locus_tag="RHZ76_004079"
     CDS             5276..6205
                     /gene="alsK"
                     /locus_tag="RHZ76_004079"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HDV2814611.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPG
                     LVSGIGKMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQPRNYPLHDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLVAMTQKYLRRPLPHQVV
                     RFIAASSSDFNGAQGAAILAHQRFLPQFCAKAP"
BASE COUNT         1597 a         1484 c         1618 g         1547 t
ORIGIN      
        1 tttttttgtg aagtcgccag catcttttct gttcttgctg tggtgatata gtggcgtctt
       61 caattcaagg acaagagaac gtgatgagcc agtcagagtt tgattcagcg cttccgaacg
      121 gtatagggtt agcgccttac ctgcgaatga agcaggaagg aatgacagaa aatgaaagcc
      181 gcatcgtgga gtggttactc aaacccggta acctgagttg tgcacccgca attaaagatg
      241 tcgcagaagc tctggcggta tctgaagcga tgatagttaa ggtatcaaag ctgctggggt
      301 ttagcggctt tcgtaactta cgcagtgcgc tggaagatta tttttctcag tcagaacagg
      361 tattgccttc cgagttggct tttgatgaag cgccgcagga tgtggtgaat aaggtattta
      421 acatcacttt acgcaccatt atggaaggtc agtcgatcgt caacgttgat gagatccacc
      481 gtgccgcccg ctttttctat caggccagac agcgggattt gtacggtgcc ggaggatcaa
      541 atgctatctg tgctgatgta cagcacaagt tcttgcgcat tggcgtacgc tgtcaggcct
      601 atcctgatgc tcacatcatg atgatgtccg cttcgttgtt acaggaagga gatgttgtgc
      661 tggtagtgac ccattccggg cgaaccagtg atgtaaaagc ggccgtagaa ctggcaaaaa
      721 agaacggggc aaagattatt tgtataaccc atagctacca ttcaccgata gcgaaactgg
      781 ccgattatat tatttgctca ccagccccgg aaacgccgtt attaggtcgt aatgcctcgg
      841 caagaatatt acaactaact ttgctggacg ctttttttgt ctctgtcgcc cagctcaaca
      901 ttgaacaagc taatattaat atgcaaaaaa ccggcgcaat tgttgatttc ttctcaccag
      961 gcgcgctgaa ataagaaaaa ggtaattccg ccatcgcgga gttacctgtc cccttaccgt
     1021 gagaacatta ttatgaataa atatctgaaa tatttcagcg gcacactcgt gggcttaatg
     1081 ttgtcaacca gcgcttttgc tgccgccgaa tatgctgtcg tattgaaaac cctctccaac
     1141 ccattttggg tagatatgaa aaaaggcatt gaagatgaag caaaaacact gggcgtcagc
     1201 gttgatattt ttgcctctcc ttcagaaggc gattttcaat ctcaattgca gttatttgaa
     1261 gatctctgta ataaaaatta caaaggtatc gccttcgctc cattatcctc agtgaatctg
     1321 gtcatgcctg tcgcccgcgc atggaaaaaa ggcatttatc tggttaatct cgatgaaaaa
     1381 atcgacatgg ataatctgaa aaaagctggc ggcaatgtgg aagcttttgt caccaccgat
     1441 aacgttgctg tcggggcgaa aggcgcgtcg ttcattattg acaaattggg cgctgaaggt
     1501 ggtgaagtcg caatcattga gggtaaagcc ggtaacgcct ccggtgaagc gcgtcgtaat
     1561 ggtgccaccg aagccttcaa aaaagcaagc cagatcaagc ttgtcgccag ccagcctgcc
     1621 gactgggacc gcattaaagc actggatgtc gccactaacg tgttgcaacg taatccgaat
     1681 attaaagcga tctattgcgc gaatgacacg atggcaatgg gtgttgctca ggcagtcgca
     1741 aacgccggaa aaacgggaaa agtgctggtc gtcggtacag atggcattcc ggaagcccgc
     1801 aaaatggtgg aagccggaca aatgaccgcg acggttgccc agaacccggc ggatatcggc
     1861 gcaacgggtc tgaagctgat ggttgacgct gagaaatccg gcaaggttat cccgctggat
     1921 aaagcaccgg aatttaaact ggtcgattca atcctggtca ctcaataaaa ctgaatgatg
     1981 ccggatgatg atgctaatga agtgtcttat ccggccaaca aaataacctg aacgtagacc
     2041 tgataaatgt agccatcagg caataatacg atatttcgtg cagagaggtt atttatggcc
     2101 acgccatata tatcgatggc ggggatcggc aagtcctttg gtccggttca cgcattaaag
     2161 tcggttaatt taacggttta tcctggtgaa atacatgcat tactaggaga aaatggcgcg
     2221 ggtaaatcca cgctaatgaa agttttatcc ggaatacatg agccgaccaa aggcaccatt
     2281 accattaata acattagcta taacaagctg gatcataaat tagcggcaca actcggtatc
     2341 gggattattt atcaggaact cagcgttatt gatgaattaa ccgtactgga aaatttatat
     2401 attggtcgtc atctgacgaa aaaaatctgt ggcgtcaata ttatcgactg gcgagaaatg
     2461 cgtgtccgcg ccgccatgat gttattacgc gtgggcttga aagttgatct agatgagaaa
     2521 gtggcgaatt tatctatcag ccacaagcag atgctagaaa ttgccaaaac gctgatgctc
     2581 gatgccaaag tcatcatcat ggatgaaccc acctcctcac tcaccaataa agaggtggac
     2641 tatctgtttc tgatcatgaa tcagttgcgt aaagagggta cggccatcgt ctatatctcg
     2701 cataagttgg cggaaattcg ccgtatttgc gaccgctata cggtgatgaa agacggcagc
     2761 agcgtttgca gcggcatagt aagcgatgtg tcaaatgacg atatcgtccg tctgatggta
     2821 ggccgcgaac tgcaaaaccg ttttaacgcg atgaaggaga atgtcagcaa ccttgcgcac
     2881 gaaacggttt ttgaggtgcg gaacgtcacc agtcgtgaca gaaaaaaggt ccgggatatc
     2941 tcatttagcg tctgccgggg agaaatatta ggctttgccg gactggtcgg ttccggacgt
     3001 actgaactga tgaattgtct gtttggcgtg gataaacgcg ctggcggaga aatccgtctt
     3061 aatggcaaag atatctctcc acgttcaccc ctggatgccg tgaaaaaagg gatggcttac
     3121 atcactgaaa gccgccggga taacggtttt ttccccaact tttccatcgc tcagaacatg
     3181 gcgatcagcc gcagtctgaa agacggcggc tataaaggcg cgatgggctt gtttcatgaa
     3241 gttgacgagc aacgtaccgc tgaaaatcaa cgcgaactgc tggcgctgaa atgtcattcg
     3301 gtaaaccaga atatcaccga actctccggg ggaaatcagc agaaagtcct gatctccaaa
     3361 tggctgtgct gttgcccgga agtgattatt ttcgatgaac ctacccgcgg catcgacgtt
     3421 ggcgcgaaag ccgaaattta caaagtgatg cgccaactgg cggacgacgg aaaagtcatc
     3481 ctgatggtgt catctgaact acctgaaatt atcaccgtct gcgaccgcat cgccgtgttc
     3541 tgcgaaggac gactgacgca aatcctgacg aatcgcgatg acatgagcga agaggagatt
     3601 atggcatggg ctttaccaca agagtaaaaa gcgaagcgag cgagaagaaa ccgttcaact
     3661 ttgcgctgtt ctgggataaa tacggcacct tttttatcct ggcgatcatc gtcgccatct
     3721 ttggttcgct gtcaccagaa tattttctga ccaccaataa tattacccag atttttgttc
     3781 aaagctccgt gacggtattg atcggcatgg gcgagttttt cgctatcctg gtcgctggta
     3841 tcgacctctc ggttggcgcg attctggcgc tttccggtat ggtgaccgcc aaactgatgt
     3901 tggcaggtgt tgacccgttt ctcgcagcga tgattggcgg tgtactggtt ggcggcgcac
     3961 tgggggcgat caacggctgc ctggtcaact ggacggggct acacccgttc atcatcaccc
     4021 ttggcaccaa cgcgattttc cgtgggatca cgctggtgat ctccgatgcc aactcggtat
     4081 acggcttctc atttgacttc gtgaacttct ttgccgccag cgtaattggg atacctgtcc
     4141 ccgttatctt ctcactaatt gtcgcgctca tcctttggtt tctgacaacg cgtatgcggc
     4201 tcgggcgcaa catctacgca ctgggcggca acaaaaattc ggcgttctat tccgggattg
     4261 acgtgaaatt ccacatcctg gtggtgttta tcatctccgg tgtttgtgca ggtctggcag
     4321 gcgtcgtctc aactgcacga ctcggtgccg cagaaccgct tgccggtatg ggttttgaaa
     4381 cctatgccat tgccagcgcc atcattggcg gcaccagttt cttcggcggc aaggggcgca
     4441 ttttctctgt ggtgattggc gggttgatca tcggcaccat caacaacggt ctgaatattt
     4501 tgcaggtaca aacctattac caactggtgg tgatgggcgg attaattatc gcggctgtcg
     4561 cccttgaccg tcttatcagt aagtaaggaa ttgaacatga aaatctcccc ctcgttaatg
     4621 tgtatggatc tgctgaaatt taaagaacag atcgaattta tcgacagcca tgccgattac
     4681 ttccacatcg atatcatgga cggtcacttt gtccccaatc tgacactctc accgttcttc
     4741 gtaagtcagg ttaaaaaact ggcaactaaa ccgctcgact gtcatctgat ggtgacgcgg
     4801 ccgcaggatt acattgctca actggcgcgt gcgggagcag atttcatcac tctgcatccg
     4861 gaaaccatca acggccaggc gttccgcctg attgatgaaa tccgccgtca tgacatgaaa
     4921 gtggggctga tccttaaccc ggagacgcca gttgaggcca tgaaatacta tatccataag
     4981 gccgataaaa ttacggtcat gactgtcgat cccggctttg ccggacaacc gttcattcct
     5041 gaaatgctgg ataaacttgc cgaactgaag gcatggcgtg aacgagaagg tctggagtac
     5101 gaaattgagg tggacggttc ctgcaaccag gcaacttacg aaaaactgat ggcggcaggg
     5161 gcggatgtct ttatcgtcgg cacttccggc ctgtttaatc atgcggaaaa tatcgacgaa
     5221 gcatggagaa ttatgaccgc gcagattctg gctgcaaaaa gcgaggtaca gcctcatgca
     5281 aaaacagcat aacgtcgtag cgggcgtgga tatgggggca acgcatatcc gcttttgtct
     5341 gcggacagca gaaggtgaaa cgctacactg cgaaaaaaag cggaccgcag aagtcattgc
     5401 tcccggcctg gtgtcgggta tcggcaaaat gattgacgag caactcaggc gctttaacgc
     5461 tcgctgtcat ggtctggtga tgggatttcc ggcgctggtc agtaaagata aacgcaccat
     5521 tatttctacg cctaacctgc cgttaacagc ggcggattta tatgatctcg ccgataagct
     5581 cgaaaatacg ctgaattgtc cggttgagtt ttcccgcgac gttaacctgc aactctcctg
     5641 ggacgtagta gaaaaccgcc ttacgcaaca actggttctg gcggcctatc tcggtacggg
     5701 gatggggttc gcagtgtgga tgaacggtgc gccgtggacg ggtgcacacg gtgtggcagg
     5761 cgaactgggt catatccccc tgggagatat gacccaacac tgcgcgtgtg gcaatcctgg
     5821 gtgcctggaa accaattgct ctggaatggc gctaagacgc tggtacgaac aacagccccg
     5881 aaattaccca ttgcacgatc ttttcgtcca tgcggaaaac gcccctttcg tccagagtct
     5941 gcttgaaaac gcggcacggg ccattgccac cagcattaat ctgttcgatc ccgatgcggt
     6001 gatcctgggc ggtggcgtga tggatatgcc cgccttccca cgcgagactc tcgttgccat
     6061 gacccaaaag tacctgcgcc gtccactgcc gcatcaggtc gtgcgcttta ttgccgcctc
     6121 atcttctgac tttaatggcg ctcagggtgc agcaatattg gcgcatcaac gttttttgcc
     6181 acagttctgt gctaaagccc catgagtaaa acgttggcac agtagcgcaa tgtcccgctg
     6241 tgccaa
//
LOCUS       DAPHBJ010000231         6177 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.233, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000231 DAPHBJ010000000
VERSION     DAPHBJ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.233"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            135..1355
                     /gene="astC"
                     /locus_tag="RHZ76_004080"
     CDS             135..1355
                     /gene="astC"
                     /locus_tag="RHZ76_004080"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HDV2814612.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            1352..2386
                     /gene="astA"
                     /locus_tag="RHZ76_004081"
     CDS             1352..2386
                     /gene="astA"
                     /locus_tag="RHZ76_004081"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HDV2814613.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            2383..3861
                     /gene="astD"
                     /locus_tag="RHZ76_004082"
     CDS             2383..3861
                     /gene="astD"
                     /locus_tag="RHZ76_004082"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HDV2814614.1"
                     /translation="MTLWINGDWITGQGALRVRRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSFAERQAVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLRRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            3858..5201
                     /gene="astB"
                     /locus_tag="RHZ76_004083"
     CDS             3858..5201
                     /gene="astB"
                     /locus_tag="RHZ76_004083"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HDV2814615.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTLPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMVLVLPQECREHAGVWCY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTQEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            5194..6162
                     /gene="astE"
                     /locus_tag="RHZ76_004084"
     CDS             5194..6162
                     /gene="astE"
                     /locus_tag="RHZ76_004084"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HDV2814616.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRRHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFKKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         1318 a         1583 c         1857 g         1419 t
ORIGIN      
        1 ttgatttccc gttagcctcc gccgtttatg cacttttatc actggctggc acgaaccctg
       61 caatctacat ttacagcgca gacattactt attgttaaca tataaataac gaattattta
      121 ctgtagaggt cgctatgtct cagccaatta cgcgtgaaaa ctttgatgaa tggatgatac
      181 ctgtttacgc tccggcaccc tttataccgg tacgtggcga aggttcgcgc ttgtgggatc
      241 agcaggggaa agagtatatc gactttgcgg gtggcattgc ggtgaacgcg ctgggccatg
      301 cgcatccgga actgcgtgaa gcgctgaacg aacaggcgag taagttctgg cataccggca
      361 acggttacac caacgagccg gtactgcgac tggcgaaaaa attgatcgac gccacgtttg
      421 ccgatcgcgt cttcttttgt aactccggtg cggaagccaa cgaagcggcg ctaaaactgg
      481 cgcgtaaatt cgctcacgac cgctacggca gccataagag cggcatcgtg gcgttcaaaa
      541 atgcgtttca tggtcgcacg ctgtttactg tcagtgcggg tgggcagcca gcctattcac
      601 aggattttgc gccactgccg gcggatattc gtcatgctgc atataacgat attaactctg
      661 ccagcgcgct gattgacgac tctacctgtg cggtgattgt cgaacccatc cagggggaag
      721 gtggtgtggt gccagccagc aacgcgtttt tacaaggtct gcgtgaattg tgtaaccgcc
      781 acaatgcgct gttgattttt gatgaagtac aaaccggcgt cgggcgcacc ggggaactgt
      841 atgcctatat gcactacggc gtgacgcctg atctgttaac taccgccaaa gcgctgggcg
      901 gcggtttccc ggtcggtgcg ttgttggcaa ccgaagagtg cgcccgcgtg atgaccgttg
      961 gcactcatgg caccacctat ggcggtaatc cgctggcctc ggcggtggca ggcaaagtgc
     1021 tggagctcat caacacacca gagatgctta atggcgttaa acagcgtcac gactggtttg
     1081 ttgagcgtct taatactatt aatcaccgct atggtttgtt cagtgaagtt cgcggcttag
     1141 gtttgctgat tggctgtgta ctgaatgccg attacgccgg gcaagcgaaa cagatctctc
     1201 aggaagcggc gaaagcaggc gtgatggtac tgattgcggg tggcaacgtg gtgcgttttg
     1261 cgcctgcgct caatgtcagc gaagaagagg tgacgaccgg actggatcgc tttgcagctg
     1321 cttgcgaaca ctttgttagc cgaggttcat catgatggtc atccgtcccg ttgagcgatc
     1381 agatgtctcg gcgctgatgc agcttgccag caaaacgggc ggcggcctga cgtcgcttcc
     1441 cgccaatgaa gccacgcttt cggcgcgtat cgaaagggca atcaaaacct ggcaaggcga
     1501 actgcccaaa agtgagcagg gctatgtgtt cgtgctggaa gatagcgaga caggcaccgt
     1561 ggcggggatt tgtgccattg aggtggcggt tgggctgaac gatccctggt acaactatcg
     1621 cgtcggcacg ttggttcacg cctcaaaaga gctgaatgtc tataacgcat tgccgacgct
     1681 gtttctcagt aacgatcaca ccggcagcag cgagctgtgc acgctgtttc tcgacccgga
     1741 ctggcgcaaa gagggcaacg gctatttgct gtcgaaatcg cgctttatgt ttatggcggc
     1801 ttttcgcgac aagtttaatg acaaagtggt tgctgaaatg cgcggggtga ttgacgaaca
     1861 cggctattca ccgttctggc aaagcctcgg taaacgcttc ttttcgatgg attttagccg
     1921 cgccgatttt ctctgcggca ccgggcaaaa ggcatttatt gcagaactga tgccgaaaca
     1981 tccgatctat acccactttt tatcccagga agcccaggac gtcatcggtc aggtacatcc
     2041 gcaaaccgcg cctgcccgcg cggtgctgga gaaagaaggt tttcgttacc gcaactatat
     2101 cgacatcttc gacggcgggc caacgcttga gtgtgacatc gaccgcgtgc gcgccatccg
     2161 taaaagtcgg ctggtggaag tggcagaagg gcagcctgcg cagggcgatt ttccggcctg
     2221 cctggtcgcc aatgaaaatt atcaccattt ccgcgtggtg ctggtgcgta ccgatccggc
     2281 aaccgagcgt ttgatattaa ccgccgcaca actggatgcc ctgaaatgcc acgccgggga
     2341 tcgcgttcgt ctggtgcgcc tgtgcgcaga ggagaaaaca gcatgacttt atggattaac
     2401 ggtgactgga taacgggcca gggcgcattg cgtgtgaggc gtaatccggt atcgggcgag
     2461 gtgttatggc agggcaatga tgccgatgcc gctcaggtcg agcaggcttg tcgggcagcc
     2521 cgtgcggcgt ttcctcgctg ggcgcggctc tcatttgctg aacgtcaggc cgttgtcgaa
     2581 cgctttgccg gattgctgga aaggaataaa ggcgaattaa ccgcaatcat tgcccgagaa
     2641 accggcaagc cgcgctggga agcggcaacc gaagtgacgg cgatgatcaa taaaatcgcg
     2701 atatcaatta aggcgtatca cgttcgtacc ggcgagcagc gtagcgaaat gccggacggc
     2761 gcggcgagcc tgcgacatcg cccgcacggc gtgctggcgg tgtttgggcc gtataatttc
     2821 cctggtcatt tgccgaacgg acatatcgtt ccggcattgc tggcaggtaa caccattatc
     2881 tttaaaccca gcgaactgac accgtggagt ggcgaagcgg taatgcgttt atggcagcag
     2941 gctggcttgc cgccgggcgt actgaacctg gtgcagggcg ggcgagaaac gggtcaggcg
     3001 ctgagtgcgc tggaggatct cgacggtttg ctgtttaccg gtagcgccaa taccggctac
     3061 cagctgcgtc gccagctctc cggtcagccg gagaaaattc tcgcccttga gatgggcggt
     3121 aataatccgc taattatcga tgaggtggcg gatatcgacg cggctgtcca tctgaccatt
     3181 cagtcggcgt ttgtcacagc cgggcaacgc tgcacctgcg cccgccgttt attgctcaaa
     3241 agcggagcgc agggcgatgc gtttcttgct cgtctggttg ccgtcagcca gcgattaacg
     3301 ccgggcaact gggatgacga accgcagccg tttattggcg ggctgatttc tgaacaggcc
     3361 gcacagcagg tggttactgc ctggcagcaa ctggaagcga tgggcggacg aaccctgctt
     3421 gcgccgcgct tattacaatc agagacatcg ttgctgacgc cggggatcat tgaaatgaca
     3481 ggcgttgctg gcgtaccaga tgaagaggtg ttcgggccgc ttttgcgcgt ctggcgttat
     3541 gattctttcg aggaagcgat tctaatggca aataacactc gcttcggact ctcttgcggt
     3601 ctggtttccc ccgagcggga aaaattcgat caactgttgc tggaggcgcg ggcggggatt
     3661 gttaactgga acaaaccgct tactggtgct gccagtaccg cgccattcgg cggcattggt
     3721 gcatccggta accatcgccc cagcgcctgg tatgccgcag attactgcgc atggcctatg
     3781 gcgagcctgg agtcggactc gttaactttg cccgcaacgc ttaaccccgg gctggatttt
     3841 tccgatgagg tggtgcgatg aacgcctggg aagttaattt cgacgggctg gtagggctga
     3901 cgcatcatta cgcgggcctg tcgtttggta atgaagcctc tacccgtcac cgttttcagg
     3961 tgtctaaccc gcgactggcg gcgaagcagg gcttactgaa aatgaaagcc cttgccgatg
     4021 cgggattccc ccaggccgtg atcccgccgc acgagcgtcc gtttattccg gtgctgcgtc
     4081 agttgggatt cagcggtagc gatgagcagg tactggaaaa agttgcacgc caggcaccgc
     4141 actggctttc cagcgtcagc tccgcttcgc caatgtgggt agccaatgcg gcaacgatcg
     4201 cgccatctgc cgatacgctg gatggcaaag tgcatctcac cgttgccaac ctgaacaata
     4261 aatttcaccg ttcgctggaa gcgcccgtca ctgaatcgct gttaaaagct atttttaacg
     4321 acgaagagaa atttagcgtc cattcggcgt tgccgcaggt agcgttgctc ggtgatgagg
     4381 gggcggcaaa ccacaatcgt ctcggcggtc attacggtga gccgggtatg caactttttg
     4441 tctacgggcg agaagaaggc aatgataccc tgccttcccg ttatccggcg cgacagactc
     4501 gcgaagccag cgaggcggtg gcaaggctga atcaggtgaa tccccaacag gtgattttcg
     4561 cccagcaaaa cccggacgtt atcgaccagg gcgtttttca taatgacgtg attgccgtga
     4621 gtaaccgcca ggtgctgttt tgccaccaac aggcgttcgc tcgccagtca cagttactgg
     4681 caaacctgcg tgcgcgggtc aatggtttta tggcgataga agttccggca actcaggttt
     4741 ccgtgtcgga tgcggtgtct acatatctgt tcaacagcca actgctgagc cgcgatgatg
     4801 gttccatggt gttggtgctg ccacaggagt gtcgggaaca cgccggagta tggtgttatc
     4861 tcaatgaact ccttgccgct gacaacccga tcagcgaact aaaagtcttt gatttacgtg
     4921 aaagcatggc gaatggcggc ggcccggcgt gcctgcggtt gcgggtggta ttgacacaag
     4981 aagaacgccg ggcggtgaat ccggcggtga tgatgaacga taccttgttt aatgcgctca
     5041 atgactgggt ggatcgttac taccgcgatc gtcttactgc tgccgatctg gccgacccgc
     5101 aattgctgcg cgaagggcgg gaagcactgg atgtattgag ccaattactg aatctcggtt
     5161 cggtttatcc gttccagcgc gagggagggg gcaatggata atttccttgc tctgacctta
     5221 acgggtaaaa aaccggttat caccgagcga gaaatcaacg gcgttcgctg gcgctggctg
     5281 ggcgatggtg tgctggaact gacgccatta acgccaccgc aaggcgcact ggtgatttct
     5341 gcggggatcc acggtaatga gacggcacct gtggagatgc tggacgcgtt gcttggcgcg
     5401 atatctcacg gcgagatccc gttacgttgg cggttgctgg tgatcctcgg gaatcctcct
     5461 gcgctgaagc aagggaaacg ttattgccat agcgatatga accgaatgtt tggcggtcgt
     5521 tggcagctgt ttgctgaaag cggggaaacc tgtcgggcgc gcgaactgga acagtgcctg
     5581 gaagattttt atgaccaggg taaagaatct gtgcgccggc accttgatct acatacagca
     5641 attcgtggct ccttgcatcc gcagttcggt gtattaccgc aacgcgacat accctgggac
     5701 gagaaattcc tgacgtggct gggtgcggcg gggctggagg cactggtgtt ccatcaggag
     5761 cctggtggta cgtttaccca tttcagcgcc agacattttg gcgcgctggc ctgtacgctg
     5821 gaactcggca aagcgttgcc ctttggacaa aacgatcttc gccagtttgc agtaactgcc
     5881 agcgcaattg ctgcgctgct atctggtgag agtgttggta tcgtgagaac gccgccgctc
     5941 cgttatcggg tggtttcgca aattactcgc cactcgccgt ccttcgaaat gcatatggca
     6001 agtgacacgc tgaattttat gccgtttaag aaaggaacgt tgctggcgca ggacggtgag
     6061 gaacgtttta ccgtaaccca tgatgtagag tatgtgttat tccctaatcc gttggtagcg
     6121 ttgggattac gcgcgggatt aatgctcgaa aaaataagct aatacttacc cgcagaa
//
LOCUS       DAPHBJ010000232         6140 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.234, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000232 DAPHBJ010000000
VERSION     DAPHBJ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.234"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1428
                     /locus_tag="RHZ76_004085"
     CDS             <1..1428
                     /locus_tag="RHZ76_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDV2814617.1"
                     /translation="LPVYDRNNLAPRIVHLGFGAFHRAHQGVYADILATEHFSDWGYY
                     EVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQIDGLETVLAAMC
                     EPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATGVIVEALARRKA
                     AGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFPSTMVDRIVPAV
                     TEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLPYEEM
                     KLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGLMLQEQAPTLKVQGVDLQD
                     YANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLALGVAGWM
                     RYVGGVDEQENPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDDLPDNSLFT
                     AKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1464..1667)
                     /gene="ydfZ"
                     /locus_tag="RHZ76_004086"
     CDS             complement(1464..1667)
                     /gene="ydfZ"
                     /locus_tag="RHZ76_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HDV2814618.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1844..2530)
                     /gene="ydfH"
                     /locus_tag="RHZ76_004087"
     CDS             complement(1844..2530)
                     /gene="ydfH"
                     /locus_tag="RHZ76_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HDV2814619.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2619..3365)
                     /gene="ydfG"
                     /locus_tag="RHZ76_004088"
     CDS             complement(2619..3365)
                     /gene="ydfG"
                     /locus_tag="RHZ76_004088"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HDV2814620.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKVEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3502..5547
                     /gene="dcp"
                     /locus_tag="RHZ76_004089"
     CDS             3502..5547
                     /gene="dcp"
                     /locus_tag="RHZ76_004089"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HDV2814621.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNKTHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPKIMPMLQHRGLNI"
     gene            complement(5591..6109)
                     /locus_tag="RHZ76_004090"
     CDS             complement(5591..6109)
                     /locus_tag="RHZ76_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HDV2814622.1"
                     /translation="MNINRDKIVQLVDTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1528 a         1480 c         1571 g         1561 t
ORIGIN      
        1 ctccctgttt atgatcgtaa taacctggcc ccaagaattg ttcatttagg ctttggtgca
       61 tttcaccgtg cgcatcaggg cgtgtatgcc gatattcttg ctacggaaca tttcagtgac
      121 tggggatatt atgaggtcaa cttaatcggc ggcgaacagc aaattgccga tttacaacag
      181 caagataatc tttataccgt tgcggaaatg tcggccgacg cgtggacggc tcgcgtcgtt
      241 ggcgtcgtta aaaaagcctt gcacgtacaa atagatggct tagaaaccgt gctggctgcg
      301 atgtgtgaac cgcaaattgc gattgtctct ctgacaatca ccgaaaaagg gtatttccac
      361 tctccggcga ccggacagtt aatgctcgat cacccgatgg tcgctgccga cgtgcaaaat
      421 ccccaccagc cgaaaacagc aacaggggtg attgttgagg cgctggctcg ccgtaaagcg
      481 gcaggacttc ccgcatttac cgttatgtca tgtgacaaca tgccagaaaa cggtcatgtt
      541 atgcgtgacg ttgtcacttc ctacgcgcaa gccgttgatg taaaactggc acaatggatc
      601 gaagataacg tgactttccc atcaacaatg gtggaccgta ttgtgcccgc agtgacagag
      661 gatactcttg cgaaaatcga acaacttacc ggtgtgcgcg atcctgctgg cgttgcctgt
      721 gaacctttcc gccagtgggt aatagaagat aattttgttg ccggacgtcc ggaatgggaa
      781 aaagcgggag cagaactggt tagcgatgta ctgccttatg aagagatgaa gttgcgcatg
      841 ctcaacggca gtcattcatt cctggcgtat ctgggttatc ttgctggata tcagcacatt
      901 aatgactgta tggaagatga acattatcgt catgcggcgt atggcttgat gttgcaggaa
      961 caagcgccga cgctgaaagt gcagggcgtt gatttgcaag attacgctaa ccgattaatt
     1021 gcacgctata gcaacccggc gctacgtcat cgaacctggc agattgcgat ggatggcagc
     1081 cagaaattgc cacagcggat gttggattct gttcgctggc atctggcgca tgacagcaag
     1141 ttcgatctgc tggcgctggg cgtcgcgggt tggatgcgtt atgtcggtgg tgttgatgaa
     1201 caggaaaatc cgatagaaat cagtgaccca ctgttacctg ttattcagaa ggctgtacaa
     1261 agtagtgccg aagggaaagc gcgcgtccag tcattgctgg cgattaaggc aatctttggt
     1321 gatgatttgc cagacaatag cttgtttact gcaaaagtga cggaagcgta cttgtcttta
     1381 ttagcgcatg gcgcgaaagc gaccgtggca aaatattccg tgaagtaaaa aaagcaatgc
     1441 cgccatcggc ggcattcaca cgcttagttc atgccgagac gaatcaggtc cagtggtgcc
     1501 agtttctctt cacaaccttc aacaactacg gtttttccgc ggcggatttg ctcagcggtc
     1561 agaccttcag tatcaatcga caggatcttg ccagtgtgac cggtgccgct aaccataaca
     1621 cggctgccag tggtgattgc gttacggtta cgatcgtagg tcgtcatagg tgttttctcc
     1681 tttctgattt acagtcgaag cccacgtaac gggcgcaata taaatactcc tgtcgagtga
     1741 aatatttttt gtttttgatc aagctcacat cttttttgat gcatgcatga gccataaggc
     1801 gggggaaaag actctcaccc cgaatgggat gagaggaaat gaattactct tcgctaaacc
     1861 agtcgctatt ttcctggcgg atctggcgca cagattcgct gatttcctgc aaatgttgcg
     1921 tcattgcacg ttctaccgca tcgccatcac gtttttgcag ggcagagaaa atatcaagat
     1981 gctggcgtaa cagcatttct ggtggagaaa cgtggtcgaa actcatatag cgcacgcgat
     2041 caacggtcgc tttcaggttc tcaatggtat cccacgccag ttgacagtcg gcaatctgcg
     2101 tcaggagttg atggaagttg tcatcaagtt caaaaaaatc atccagttgc ttgcgctcaa
     2161 tggcaatgcg ttgctggtga agattttgtt ccagttggta gcactggctt tcggtaatca
     2221 tgctcgccgc ccgacgcgcc accgcgcact caatggcctg acggataaaa ctgccgttgc
     2281 gcacctgggc catggaaatt ttgttgacgt agctgccacg ctgcggacga atttgaatca
     2341 ggccgttttc cgccagttta ataaaggctt cacgaaccgg ctggcgtgac acattgaaac
     2401 gaacagaaac ttctttttcc gacaacggtg tgcctggtgc aatcaggcaa tggacaatgt
     2461 cgcgacgaag aatacgataa atctgctgat tgacaggctg tgtgggatta agttgcgttt
     2521 cgacggtcat tcgttcttgc ttgtgagtga gttaactgca tgagtctacc acttttttgt
     2581 gacaagcaat aacccggcag ttacgccggg tataaaaatt actgacggtg gacattcagt
     2641 ccggcatagc tttgggtaac cggcatcatt tccagggtat tgatattgac gtgagcaggc
     2701 agcgttgaca cccaccagac ggcttcgctg acatcttctg gcgtcaatgc aacggtattt
     2761 tgataggttt tttccacttt accgtcatcg cctttaaagc ggacattgga aaactcggtg
     2821 ccacccacca gacccggctc gatgtcggtg acgcgcaccg ccgtaccatg cagatccgta
     2881 cgcagattca ggctaaactg acgaacaaac gctttcgtcg caccgtaaac gttaccaccg
     2941 gcatacggcc agctacctgc cgttgagcca atgttaataa tatgaccatg attacgttca
     3001 accatacccg gtaagacggc gcgcgtcata tataccaggc ctttattgtt ggtatcaatc
     3061 atcgtttccc agtcttcaac gctggcttta tgcgcaggct ccatgcccaa cgccaggccg
     3121 gcattattta ccaggatatc aatattgctc cactcggcag ggagcgatgc cagcatctct
     3181 tcaatagcgg cgcggttgcg aacgtccagt tgggcgatat acagattatc tcccagttcg
     3241 tcttttaact cctgcaaccg ttcctgacga cggccagtgg cgataacttt atgcccttgt
     3301 tgaataaaac gacgagtaat gcattcacca aaacctgccg ttgctccagt tactaaaacg
     3361 atcatctcac tgttcctcaa cgcttttgtg tgtcaataac atagcacggg atgcttaaga
     3421 gcgggtaata ctggattgca agattgcact ccgttatcag taagcctacg ctatggaaca
     3481 cctcgcgttc aggagaagaa aatgacaaca atgaatcctt tccttgtgca aagcacactg
     3541 ccgtatctgg ctcctcattt tgatcaaatt gccaatcatc actatcgccc ggcattcgat
     3601 gagggaatgc agcaaaagcg ggcagaaatt gctgccatcg cgcttaaccc gcaaacgcct
     3661 gatttcaaca atactattct ggcactggaa caaagcggag aattacttac ccgcgttacc
     3721 agcgtctttt ttgcgatgac tgcggcgcat accaatgatg aattacagcg tcttgatgaa
     3781 cagttttccg ctgaactggc ggaactggct aatgatatct atctgaacgg tgaattattc
     3841 gcgcgggtag atgctgtctg gcagcgccgt gaatccctgg ggcttgatag tgaatccatc
     3901 cgcctggtgg aggtgattca tcaacgtttt gtccttgccg gagccaaact tgcgcaagct
     3961 gataaagcaa aattaaaagt actgaataca gaagctgcga cactgaccag tcagtttaac
     4021 cagcgattac tggcagcaaa taaatccggc ggtctggttg tgaacgatat cgcgcagctg
     4081 gcaggaatga gtgagcaaga gattgcgctg gcggcagagg cggctcgcga gaaaggtctg
     4141 gataacaaat ggctgattcc gctgctgaat accacccaac aaccggcgct tgccgagatg
     4201 cgcgatcgtg cgacgcgtga aaaactgttt attgcgggct ggacgcgagc ggaaaaaaat
     4261 gatgccaatg atacccgcgc tatcattcaa cgtctggtag agattcgcgc acagcaggcg
     4321 aaactgcttg gttttcctca ttatgccgca tggaaaatcg ccgatcagat ggcaaaaaca
     4381 cctgaagcag cacttaactt tatgcgggaa attgttccag cggcgcgtca acgtgcgagc
     4441 gatgaattag cctccataca ggcggttatc gataagcagc agggcgggtt tagcgcgcag
     4501 ccgtgggact gggcatttta tgcggaacag gtacggcggg agaaatttga tcttgatgag
     4561 gcgcagctca agccatattt tgaattaaac acggtgttaa atgaaggtgt attctggacc
     4621 gcgaatcagc tcttcggtat taagtttgtc gaacgttttg atattcctgt ctaccatcct
     4681 gacgttcgtg tgtgggaaat ttttgatcat aatggcgtgg ggctggcgtt attttacggt
     4741 gatttcttcg cccgtgattc aaaaagcggc ggtgcatgga tgggcaattt tgttgagcaa
     4801 tcaacgctta ataaaacaca tccggtaatt tataacgtct gcaattatca gaaacccgct
     4861 gccggtgagc ctgcgttgtt actctgggat gatgtcataa ccttattcca tgaatttggt
     4921 catacgctgc acggcctttt tgcccgccag cgttatgcca cgctttccgg caccaacacg
     4981 ccgcgtgatt ttgtcgaatt tccgtcgcaa atcaacgaac actgggcaac gcatccgcag
     5041 gtattcgctc gctacgcccg gcattatcag agcggggcag caatgcctga cgaactgcaa
     5101 cagaaaatgc gtaatgccag cctgttcaac aaagggtatg agatgagcga actgcttagc
     5161 gccgcacttc tcgatatgcg ctggcattgc ctggaagaaa acgaagcaat gcaggatgtc
     5221 gatgattttg aattgcgggc gctggtggcg gaaaatatgg atcttcctgc tataccgcca
     5281 cgctatcgca gcagttattt cgcccatatt tttggtggcg gatatgctgc aggttattac
     5341 gcttatctgt ggacgcaaat gttggccgat gatggttatc agtggtttgt tgagcagggc
     5401 ggattaacgc gtgaaaatgg gctgcgtttt cgcgaggcga tcctttccag aggtaacagc
     5461 gaagatctgg aacgcctgta tcgacaatgg cgcggtaagg cacctaagat tatgccgatg
     5521 ctgcaacatc gtggcttgaa catataaaca ccaccaatgg ctggcctgag atatcgccag
     5581 gccagcctgg ttatagcagt aattgcagca gctgggcgag ggcaaacctc accgctaaag
     5641 ctaataccgt ttcgcagtcg ccagcaaaat gcataaccgc agtgtagttc tggcctttaa
     5701 tatgccacgc aaaccagacg gtacccgctg gcgtaccatc ttcaccgccc tccggtccgc
     5761 cgtagccggt aatggcaata ctgacatcag catccgcacg ctctatggca ccggttgcca
     5821 tttctgctgc cactttctca ctcaccgcag aatatcgttc aagagattgc tggcttacgc
     5881 tgaggatttt catctttgcc tgatcggtga aagtaacaaa gcctgcaccg taaaatttgg
     5941 gtgtatcttc agctgcacac agggcgctag ccaacttacc gccggtgcat gattcggcgg
     6001 tagttaagcg taattgatcc gcgataagtc tttgactcaa cgcggatgtc aggttttcaa
     6061 tagtatcggt atctacaagt tgtacaattt tatctctatt aatgttcact gttttttgtc
     6121 cacgctttta tatgtaaagg
//
LOCUS       DAPHBJ010000233         6089 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.235, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000233 DAPHBJ010000000
VERSION     DAPHBJ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.235"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            40..816
                     /gene="exuR"
                     /locus_tag="RHZ76_004091"
     CDS             40..816
                     /gene="exuR"
                     /locus_tag="RHZ76_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HDV2814623.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1161..1823
                     /locus_tag="RHZ76_004092"
     CDS             1161..1823
                     /locus_tag="RHZ76_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV2814624.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1827..2210
                     /gene="mzrA"
                     /locus_tag="RHZ76_004093"
     CDS             1827..2210
                     /gene="mzrA"
                     /locus_tag="RHZ76_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HDV2814625.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPQGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2357..2725
                     /locus_tag="RHZ76_004094"
     CDS             2357..2725
                     /locus_tag="RHZ76_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HDV2814626.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2763..3068
                     /locus_tag="RHZ76_004095"
     CDS             2763..3068
                     /locus_tag="RHZ76_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDV2814627.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3071..3475
                     /locus_tag="RHZ76_004096"
     CDS             3071..3475
                     /locus_tag="RHZ76_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDV2814628.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3465..3764
                     /locus_tag="RHZ76_004097"
     CDS             3465..3764
                     /locus_tag="RHZ76_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HDV2814629.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSANRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3950..4342
                     /locus_tag="RHZ76_004098"
     CDS             3950..4342
                     /locus_tag="RHZ76_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDV2814630.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4412..5398
                     /gene="yqjG"
                     /locus_tag="RHZ76_004099"
     CDS             4412..5398
                     /gene="yqjG"
                     /locus_tag="RHZ76_004099"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HDV2814631.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLRADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVEKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5691..6055
                     /locus_tag="RHZ76_004100"
                     /pseudo
     CDS             5691..6055
                     /locus_tag="RHZ76_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
BASE COUNT         1508 a         1505 c         1547 g         1529 t
ORIGIN      
        1 tatgataact ttgtccgcat tccactccgg gagacgcata tggaaatcac tgaaccacgc
       61 cgtttgtatc aacaacttgc cgctgacctg aaagagcgca tcgaacaggg cgtctatctg
      121 gtgggtgata aactgcctgc cgagcgcttt attgccgatg aaaagaacgt cagccgtacg
      181 gtagttcgtg aagccatcat catgctggaa gttgaaggct atgtcgaagt gcgtaaaggt
      241 tcaggtattc acgtggtttc caaccagccg cgccatcagc aggctgcgga caataatatg
      301 gaattcgcca attacggtcc gtttgagttg ctccaggctc gccaactcat cgaaagtaat
      361 attgccgagt tcgcagcaac tcaggtaacg aaacaggaca tcatgaaatt gatggccatc
      421 caggagcagg cgcgcggcga acaatgcttc cgtgattccg agtgggattt gcagttccat
      481 attcaggtag ccctggcgac gcagaactcc gccctggcgg ctatcgttga aaaaatgtgg
      541 acccagcgta gccataaccc gtactggaaa aaactgcacg aacacattga ttcccgtacc
      601 gtcgataact ggtgtgatga ccacgatcaa atcctcaagg cgctgattcg caaagatcct
      661 catgctgcta agctggcaat gtggcagcac ctggaaaaca ccaagatcat gttatttaac
      721 gaaaccagcg acgacttcga gttcaatgcc gaccgttatc tgttcgccga aaacccggta
      781 gtgcatctcg atacggccac cagtggcagt aaatgaaata cctgtccgac aggcgctgcg
      841 acgcgcctgt ttgtctcgtc ggtaagcgaa aagtataaag tgtcagcctg tgtaaatcct
      901 ctcgccaccc tcccctgcat tcagcaaaat cagactccac ggacatgtaa ttttgataac
      961 gaacaacgtt gacctttgtt acaattagat tcaatttgaa tttatgtttt tgaatgcttt
     1021 cttatctcac gatttaacag ggaatagttc aggctgtgtt gatgtatcaa acccgcagaa
     1081 cataccaaaa cagcaataac attgcggtag tgcatctttt aaaaccagcg tggcgttaac
     1141 cgattcacca ggaataatga atggaacttt tgacccaatt gctgcaagcc ctgtgggcgc
     1201 aggattttga aaccctggcc aatccgtcga tgattggcat gttgtatttt gtcttgtttg
     1261 taattttgtt ccttgaaaac ggcttgcttc cggcggcctt tttaccgggc gacagtttac
     1321 tggtattggt cggcgtgttg attgcgaaag gcgcgatggg ctatccgcaa acgattctgc
     1381 tgctgaccgt tgccgccagc ctcggctgct gggtcagcta tattcagggg cgatggctgg
     1441 gcaatacccg caccgtacaa aactggctat ctcatttacc cgcgcattat catcaacgcg
     1501 cacaccatct ttttcataaa cacggtttat cggcgctgtt aattggtcgc tttattgcgt
     1561 ttgtcagaac actgctgccg acgattgccg ggttatcagg gctgaataac gcgcgctttc
     1621 agtttttcaa ctggatgagc ggtctgctgt gggtattgat cctgacaact ctgggttaca
     1681 tgctcggcaa aacgccggta tttttaaagt acgaggacca gctgatgtca tgcctgatgc
     1741 tgctcccggt ggtgctgctg gtgtttggcc tggcaggttc tctggtcgtg ttatggaaaa
     1801 agaaatatgg aaatcggggg taagggatgc aaatacctcg catgtcgctt cgccagctag
     1861 cctggtccgg cgctgtttta cttctggtcg gcacgctgtt gctggcctgg tccgcggttc
     1921 gccagcaaga gtctacgctg gcgattcgtg ccgttcatca aggcacaacg atgccagacg
     1981 gtttttcaat ctggcatcac cttgacgctc atggcattcc tttcaaaagt atcaccccca
     2041 aaaacgacac tctgttaatt acctttgact ccagcgacca gagcgccgct gcaaaagcgg
     2101 tcctcgacag aacattgccc caaggctaca tcattgcgca gcaggacaat aacagtcagg
     2161 ctatgcagtg gctgacccgg ctacgggata actctcatcg cttcggataa cttcctgtta
     2221 tccgaaacag atcactcact ttggtgattt caccgtaact gtctatgatt aatgaggcgg
     2281 tggttaacac taccgcttcg ttgtctggac aatcgttcct ttgtaatagg tccgaatcac
     2341 aatggaaggt tcaagaatga aataccgcat cgctttagct gtttctcttt ttgctcttag
     2401 tgctggtagt tatgccacta ccctgtgtca ggaaaaggag caaaatatcc ttaaggagat
     2461 cagctatgcc gaaaaacacc aaaaccagaa tcgtattgac ggtctgaata aagccctgag
     2521 tgaagtccgg gccaactgtt cagatagcca gctgcgtgcc gatcatcaga agaaaatcgc
     2581 aaagcagaaa gatgaggtgg cggaacgcca gcaagattta gccgaggcga agcaaaaagg
     2641 cgatgccgat aagattgcca aacgcgaacg gaaactggca gaagcgcagg aagagctgaa
     2701 aaagctggaa gcgcgcgact actaactcac aatagtcact acttactcac ctggagaaaa
     2761 ctatgtcgaa agaacacact acggaacatc tgcgtgctga gttgaaatcc ctttccgata
     2821 cgctggaaga ggtgcttagc tcatctggcg agaagtcgaa agaagagttg agtaagattc
     2881 gtagcaaagc ggagcaggca ctgaaacaga gccgttatcg cctgggtgaa accggtgatg
     2941 ccatcgccaa acaaacccgt gtcgcggcgg cgcgtgccga tgagtatgtg cgtgaaaatc
     3001 catggacggg cgtgggcatt ggcgctgcaa tcggtgtagt gctcggcgtt ctgctgtcgc
     3061 gtcgttaatt atggcggaca ctcatcacgc gcaagggccc ggtaaaagcg ttctgggcat
     3121 cgggcagcga attgtttcca tcatggttga aatggtggaa acacgtctgc ggctggcggt
     3181 ggttgagctg gaagaggaaa aagctaatct ctttcaactt ttactgatgc tgggcctgac
     3241 gatgcttttc gctgcatttg gtcttatgag cctgatggtg ctaattattt gggcggttga
     3301 tccgcaatat cgcctgaatg cgatgattgc caccaccgtg gtgttgctgc tactggcact
     3361 gattggcggt atctggacgt tacgtaaatc gcgtaaatcc accttgctgc gccatacacg
     3421 ccatgagctg gcaaacgatc ggcagctgct cgaggaggag tcccgtgagc agtaaagtcg
     3481 aacgtgaacg acgtaaggcg caactgctta gccagatcca gcaacaacgg ctggatcttt
     3541 ctgccaatcg tcgtgaatgg ctggaggcaa caggcgctta cgatcgtcgc tggaatatgc
     3601 tgctaagtct gcgctcctgg gcgctggttg gcagtagcgt gatggcgatc tggacgattc
     3661 gccatcctaa tatgctggtc cgctgggcca gacgcggttt tggcgtatgg agcgcctggc
     3721 gtctggttaa aacgaccctc aagcagcaac agcttcgcgg ttaacttccc tctggccgga
     3781 gccattccgg ccgtacccat caaatttttt gaagattttt gacagttttc cttgctaaca
     3841 atcatcattc accacgttta tgattctctc catcgacagc aacgacgcta ataccgcgcc
     3901 cttgcacaaa aaaacaatca gcagcctgag tggcgcagtg gagagtatga tgaaaaaatt
     3961 agaagatgtt ggtgtactgg tagcacgcat tttaatgccg attctgttta ttaccgctgg
     4021 ctggggaaaa attactggct acgcgggtac ccaacaatat atggaagcaa tgggcgtccc
     4081 gggttttatg ctgccgctgg tgattctgct tgagttcggt ggtggtctgg caatcctgtt
     4141 cggtttcctg actcgcacca ctgccctgtt tactgcgggc tttacgctgc tgacggcatt
     4201 tttatttcac agcaactttg ctgaaggcgt caactcgctg atgttcatga aaaacctgac
     4261 aatttctggc ggattcctgc tgctggcaat taccggtccg ggcgcgtata gcatcgaccg
     4321 cctgctgaat aaaaagtggt aagcacgcgc tatacttaac gataaaaaac gaggaggaag
     4381 ctcctcgttt ttgctattgg aggagagaaa aatgggtcaa ctgattgacg gcgtctggca
     4441 tgacacctgg tacgatacca aatctaccgg cggtaaattt caacgttcag cttccgcatt
     4501 tcgtaactgg ctcactgccg atggcgctcc tggccccact ggcacaggcg gttttatcgc
     4561 agagaaagat cgttatcatc tctatgtttc actcgcctgc ccgtgggcgc accgcacgct
     4621 gatcatgcgc aaactcaaag gactggaacc gtttatttcc gtttccgtag tgaacccgct
     4681 gatgctggaa aacggctgga cctttgatga cagttttccg ggagcaaccg gagacacgct
     4741 ctatcaacat gaatttttgt atcagcttta tctccgcgcc gatccccact acagcggacg
     4801 agttactgtt cccgtgctgt gggacaaaaa gaaccacacc atcgtcagca acgaatcagc
     4861 agaaatcatc cgcatgttta ataccgcgtt tgatgcgctg ggcgcgaaag cgggtgatta
     4921 ctacccacca gccctgcaaa cgaaaattga cgaacttaac ggctggattt atgacaccgt
     4981 taacaacggc gtgtataaag ctggttttgc caccagccag caagcctacg acgaagcggt
     5041 ggagaaagtg tttgaatcgc tggcgcgact ggaacagatt ttaggtcagc atcgttacct
     5101 gaccggcaac cagctaaccg aagccgatat tcgcctgtgg accacgctgg tccgttttga
     5161 tccagtgtat gtgacccact tcaagtgtga taagcaccgc atcagcgatt accttaatct
     5221 gtatggcttc ctgcgcgata tctaccagat gccgggaatt gccgaaacag tcaatttcga
     5281 tcacatccgt aatcattact tccgcagcca taagaccatc aaccctacgg ggattatttc
     5341 aattggtccg tggcaggatc tcgatgaacc gcatggacga gatgttcgct tcggttaaaa
     5401 aaaggcacct tacgaggtgc ctctgataat tagctaagtg catgttgttc atgtcggatg
     5461 cggcgtgaac gccttatcaa acctacaaaa catttatatt caatatgttg caatgaccgc
     5521 gcaggccaga taagcgtagc gcatctggcg attttgcgct tgtcatcagt cttagcacgc
     5581 cttcatagcc ctttattaat tccttgaacg aatatttact gccatttcat tcacgtctat
     5641 tcttaatttg ctgctttaaa aacaagtgat tgagcaaaat tgaggcaaaa atggactggt
     5701 atctgaaagt actaaaaaat tatgtcggtt tccgggtcgt gcgcggcgca aagagtactg
     5761 gatgtttatt ctggtcaaca tcatctttac gttcgtgctg gggctgctgg ataaaatgtt
     5821 aggctggcaa cgcgctggtg gcgaaggcat cctgacgaca atctacggta ttctggtgtt
     5881 tttaccgtgg tgggcagttc agttccgccg cctgcacgac accgaccgct cggcgtggtg
     5941 ggcactgcta ttcttaatcc cgtttatcgg ctggcttatt atcatcgtct ttaactgcca
     6001 ggcgggtacg ccaggcgaga accgctttgg accagatcca aaactggaac cttaaaacaa
     6061 atctgctcgc cggatgtctg actcatccg
//
LOCUS       DAPHBJ010000234         5971 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.236, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000234 DAPHBJ010000000
VERSION     DAPHBJ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.236"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(45..236)
                     /gene="yaiA"
                     /locus_tag="RHZ76_004101"
     CDS             complement(45..236)
                     /gene="yaiA"
                     /locus_tag="RHZ76_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HDV2814632.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(286..810)
                     /gene="aroL"
                     /locus_tag="RHZ76_004102"
     CDS             complement(286..810)
                     /gene="aroL"
                     /locus_tag="RHZ76_004102"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HDV2814633.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(987..1451)
                     /locus_tag="RHZ76_004103"
     CDS             complement(987..1451)
                     /locus_tag="RHZ76_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDV2814634.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAAINPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRGQM"
     gene            1571..2380
                     /gene="proC"
                     /locus_tag="RHZ76_004104"
     CDS             1571..2380
                     /gene="proC"
                     /locus_tag="RHZ76_004104"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDV2814635.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLNEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(2397..3512)
                     /gene="adrA"
                     /locus_tag="RHZ76_004105"
                     /gene_synonym="dgcC"
     CDS             complement(2397..3512)
                     /gene="adrA"
                     /locus_tag="RHZ76_004105"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HDV2814636.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(3614..3934)
                     /gene="psiF"
                     /locus_tag="RHZ76_004106"
     CDS             complement(3614..3934)
                     /gene="psiF"
                     /locus_tag="RHZ76_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HDV2814637.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(4053..5468)
                     /gene="phoA"
                     /locus_tag="RHZ76_004107"
     CDS             complement(4053..5468)
                     /gene="phoA"
                     /locus_tag="RHZ76_004107"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HDV2814638.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     SGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNAVTEVNQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(5569..5829)
                     /gene="iraP"
                     /locus_tag="RHZ76_004108"
     CDS             complement(5569..5829)
                     /gene="iraP"
                     /locus_tag="RHZ76_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HDV2814639.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         1403 a         1595 c         1444 g         1529 t
ORIGIN      
        1 ccttgtagtg gcactcagaa tcccttccga gcagtctgat gcggtcactc tttgtcaggg
       61 tcctcatagc gttttttcgc atccatcgct tcctgagcgg tcggatgttc actgatcaac
      121 gagtctggtt taggatgatc ggctctgagt tggtaccagg ttaccgtctg tcctggtttt
      181 cctttttcaa tcgtcactat atatgcttca cgaggataag gcggtttcgt tggcatcgtt
      241 cttccttatt tcacgggatg aacgttaagt ataggcgctc gaaaatcaac aattgatcgt
      301 ctgtgccagg gcgctacgaa tttcagaaat cacctggctg ggttcgtttg ttgcgtcgat
      361 gataatatgc gcaacttcgc gatatagcgc atcgcgttct tccagcactt cctgaacttc
      421 ttcgctcagc ggttttcccg ttaaggttgg ccgtaaatct tcttccggtg cagcttgcag
      481 tcggttaacc aggactgata ctggcgcaca caaataaacc acgatcccgt tattttgcat
      541 gaagtgacga ttaaattccg tcagaataat gccgccgcct gtagcgataa cggtggatgg
      601 cgcagttacc gcttccagcg ccgccgtttc tctggcgcga aatcccgccc actcttccct
      661 ttcgacgatc tccgcgaccg tcatattgag ctgtgattgc aaccactgat cggtatcgac
      721 aaaccgacgg ttaagcgaat cggcaagggc cattccgacc gttgttttac cacagccccg
      781 aggcccgatc agaaaaagag gttgtgtcat cgtgggtttt ccccaatagg tcgcaatgcg
      841 gcgaaagccg gtgtcatgag aatagcgatc ataccatcaa actagtacaa tttcgattgt
      901 aaaggaaaaa ttccacttaa agtgaaaatc tcaatacacc ccttactata ccaataaata
      961 ttcaagaatg aagtgtaaat aataaattac atttggccgc gactacgttg cacttccagc
     1021 caccatttct ccagctccgc agcaaaggcc tggcggtcac gttgtgaaag actatctggt
     1081 ccgccggtct ggatcccact ggcacgtaag gtatccataa aatcgcgcat cgtcaggcgc
     1141 tcacgaatgg tcgctggcgt gtaacgttcg ccgcgcggat taatcgccgc agcgcctttc
     1201 tcgatggctt cagcagccaa aggtatatct gcggtgatca ccaaatcgcc cgcttcacac
     1261 tgccggacaa tttcgttatc ggcaacgtcg aaacctgccg cgacgcgcag cgtacgaata
     1321 aatcgcgatg gcggcacgcg taaactctgg tttgctacca gtaccagcgg catctgcata
     1381 cgttccgccg cgcgatacaa aatctcttta attacattgg gacacgcgtc ggcatccacc
     1441 caaattgtca taaagtcatc ctttgttggg taatcctcta ttgtgtcgcg cttttgcctt
     1501 ccggcatagt tctgtttatg cttctgccag cgattatcaa aacaatgaat ttcacggcag
     1561 gagtgaggca atggaaaaga aaatcggttt tattggctgc ggcaatatgg gaaaagccat
     1621 tctcggcggt ctgattgcca gcggtcaggt gcttccaggg caaatctggg tatatacccc
     1681 ctccccggat aaagtcgccg ccctgcatga ccagttcggc atcaacgccg cagaatcggc
     1741 gcaagaagtg gcgcaaatcg ccgacatcat ttttgctgcc gttaaacctg gcatcatgat
     1801 taaagtgctt aacgaaatca cctccagcct gaataaagac tctctggtcg tttctattgc
     1861 tgcaggtgtc acgctcgacc aacttgcccg cgcgctgggc catgaccgga aaattatccg
     1921 cgccatgccg aacactcccg cgctggttaa tgccgggatg acctccgtaa cgccaaacgc
     1981 gctggtaacc ccagaagata ccgctgatgt gctgaatatt ttccgctgct ttggcgaagc
     2041 ggaagtgatt gctgagccga tgatccaccc ggtggtcggt gtgagcggtt cttcgccagc
     2101 ctacgtattt atgtttatcg aagcgatggc cgacgccgcc gtgctgggcg ggatgccacg
     2161 cgcccaggcg tataaatttg ccgctcaggc agtaatgggt tccgcaaaaa tggtgctgga
     2221 aacgggagaa catccggggg cactgaaaga tatggtctgc tcaccgggag gcaccaccat
     2281 tgaagcggta cgcgtactgg aagagaaagg cttccgtgcc gcagtgatcg aagcgatgac
     2341 gaagtgtatg gaaaaatcag aaaaactcag caaatcctga tgactttcgc cggacgtcag
     2401 gccgccactt cggtgcggtt acgtccggct ttctttgctt tgtaaagcgc caaatctgcc
     2461 gatttcaacc actcacgata gtgactcatt tgtgggttca gcggcgcaac ccccacacta
     2521 atccgtaaag ttacctgtgg cgtattcggc aaacgtaatg tatttagccc ttcatgcacc
     2581 cgtaacatgg cggtaatagc gctctcagct ggcgtaccgg acatgattac tgcaaactca
     2641 tcgccgccaa accgaccaat cacatcgcta ccgcgcaggg taatttgtaa ctgtcgggta
     2701 agcgccacta tcgcttcatc gcccacatca tggccccagg tatcgttgat gctcttgaaa
     2761 tggtcgatat cgataatcag taacgttgca tcgcgattat gtcgccgaca gttatcaaat
     2821 tcattgcgta acatagtttc ccaatgacgt cggttataca cgcccgtcat gccgtcgcgg
     2881 gtactcatga cctgcaacct gcgtttatgt tccgccagtt tggttgccgt ctggtagctg
     2941 acccagccaa acagcagagg ataaatgaca ataatgggaa gggagagcca ccattccagc
     3001 ggcgcactat tgaacgacac ggtaatgccc gtcagctcga gggtgacaag gcaggaaacc
     3061 accatcaaca ccagacccgc gacaaacaga cgggggccgc ctgcccccat caaattcaga
     3121 cacataatca tcaacatcgc ggtggaaggc agcacgttta cgcccattac gcctacccac
     3181 attcccgcta atactgcatc ggtttttaaa ttgtaaattt cccggctaag cggatcgacg
     3241 gccctgctcg ctatctgcca ggctaaatgc ggccagacga acgcccagcc gaccaacacc
     3301 agccaccacc agcccggcgg cgggtgtgaa accagcgttg aagcaatcgg taagaacatg
     3361 ccagccaggc caaccgcgcg gggtagtctg acgcgacggg cgaagcgcag cccggaccgc
     3421 tgatgttcgt tttgaggagt taaaggaggt tcctccccat gcgccgccgc ttttttgaaa
     3481 aagttttcat cattcattat ttttgggaac attcagaaac aattttccca aattatagag
     3541 acggagctaa aggcagaagt atgaaatttc gggtgagcca ttttcgtagc tcacccgtta
     3601 gcactatcaa gtatcaggcg gctttcttga ggcaggcact cataaactta ttacgatcat
     3661 cccctttcag agattgttgc gttgcttgat tattacattc gcgcatcttt tgctgctgtg
     3721 gcgtcaaact tttttcgcca ggcgcagact tgctgttctt caggcaatca ctcatgtagg
     3781 tcttacgagc atcccctttc aacgcctgcg ccgtcgcctg ctgattacag gaggtcatac
     3841 gctgttgttg tggggttaaa gttctctcgg cagcgccgac agtggttaaa aaaaccagac
     3901 caaaaagcaa ggtaaccagt aatgttattt tcatagcacc atccctcttc atgttttaac
     3961 catgagcgtt tgcgcccgtg atctgccatt aagtctggtt gctaacagca aaaaaaccac
     4021 ccggcagcga aaattcactg ccgggcgcgg ttttatttca gccccagggc ggctttcatg
     4081 gtgtagaaga gatcggtctg gtcggtcagt ccaacaacat tggcggcatg cgggccatac
     4141 gccgcaatac gcaactgact gcctgtatgt tcttgtgaat cctcttcaga gttcccgtaa
     4201 ctcatcacca tcactgcgcc atctttggta tttagcgcct gggtgaggcc cggagctttg
     4261 gtatccggcg caacaatctg gctggcgtgg gcgtgatcag cggtgactat gaccagcgtg
     4321 ttaccctcct ttttagcgaa ttccagcgcc cgttgtacgg cttcatcgag atcgaccgtc
     4381 tcgccaattt gcccacaagg attcgcagca tgatcctgtt tatcgattga cgcaccttca
     4441 acttgcagga aaaagccttt ctcattttta ctcaacaatt caatggcttt gtcggtcatc
     4501 tgcgccaggg ttggtacact gtcattacgt tgcggattag gcgtacaggt gactgcgggc
     4561 ttatcgatat tgccatggta cgttgctttc ggtcctagcc agcgcactgg catattgccg
     4621 tcagcaaaca ggccaagcag gggtttttgc tgattcactt ccgtcaccgc atttagcgag
     4681 gcggcatcgt tcaccaactg ataaccacgc gcctgtgcct gttcacgcag cgtttttccc
     4741 tgccattcac cggcggttgc cgtttcagca aaggtttttg cgccaccgcc aagcgtaacg
     4801 tcggcacgag cgttaagcag ctgttcggta atcgatcctt ttccgccttt ttccagagcg
     4861 ttacccggac atttttcact ggtcgcgctc ggaccgtagc atttgcgcga ggtcacatgc
     4921 gccaccagcg cagcgggcgt ggcatcctgc aactctgcgg tagaaacgtt accggtcgcc
     4981 agacctgagg cttttgccat ttccagaatc gttgggtgat ctttttcgtg aatatcgacg
     5041 cccagcgcgc cgttataggt tttgacaccg gttgaccagg cggttgctga tgcagccgag
     5101 tcggtgacgt agtccggttt gccggttttt ttattcagcg catagtgagt gtattgcccg
     5161 gtaagcggta aggcatcgat acctttaaaa aagccgcccg caccttcggc ataattacgt
     5221 gcggcagtaa tttccgaatc ccccatccca tcgccaatca gcaaaataat attttttgca
     5281 ggtttatcgc taagagaatc acgcagagcg gcggtctgat cacccgttaa gcggcgagca
     5341 ccgccgggtg cagtaatatc gccctgagca gcccggtttt ccagaacagg catttctggt
     5401 gtccgggctt ttgtcacagg ggtaaacagt aacggtaaga gtgccagtgc aatagtgctt
     5461 tgtttcactt tattttctcc atgtacaaat acattaaaaa ataaaaacaa agcgactata
     5521 agtctcggcc gtgacaactt tatgacagct gttgaaaaga ttaacttttt actgacgagg
     5581 atgcttcaat aacttcttta cgtaatcgcg cagcagctcc gtatcgtcgt caggaatgct
     5641 ggcatcgggc tttacctcgt acagcgcccc ctctacctga tcaatcaacc gctgttggtc
     5701 attttgcgcc atattgcgaa gcattgcagt gacgataatc tccaaagctt ctacctgcgc
     5761 acacagttct ttcgactctt cttctttttg ggcaagctta aataacaact cagcaatgag
     5821 atttttcatg tctgtatttc cttatccaaa gtatggagaa gttaacatta tgtttcacaa
     5881 cttcatagtg tctggaagta ttatttttat gacaaaggaa atactttgcg caaatatttt
     5941 ttgtttgatt accagcgaaa cgtttctctt a
//
LOCUS       DAPHBJ010000235         5941 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.237, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000235 DAPHBJ010000000
VERSION     DAPHBJ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.237"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..376)
                     /gene="yeiL"
                     /locus_tag="RHZ76_004109"
     CDS             complement(<1..376)
                     /gene="yeiL"
                     /locus_tag="RHZ76_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HDV2814640.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALSMKHYRPLLLNDTLFL"
     gene            506..1447
                     /gene="rihB"
                     /locus_tag="RHZ76_004110"
     CDS             506..1447
                     /gene="rihB"
                     /locus_tag="RHZ76_004110"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HDV2814641.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            1547..2797
                     /locus_tag="RHZ76_004111"
     CDS             1547..2797
                     /locus_tag="RHZ76_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDV2814642.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(2853..3941)
                     /locus_tag="RHZ76_004112"
     CDS             complement(2853..3941)
                     /locus_tag="RHZ76_004112"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDV2814643.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3944..4801)
                     /gene="nfo"
                     /locus_tag="RHZ76_004113"
     CDS             complement(3944..4801)
                     /gene="nfo"
                     /locus_tag="RHZ76_004113"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDV2814644.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(4875..5924)
                     /locus_tag="RHZ76_004114"
     CDS             complement(4875..5924)
                     /locus_tag="RHZ76_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HDV2814645.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
BASE COUNT         1457 a         1503 c         1541 g         1440 t
ORIGIN      
        1 gtaaaaatag cgtgtcgttt aataacagcg ggcggtaatg tttcatagag agcgcaaggc
       61 accaacactc ttcaatagcc tgcaccgcac gcggttcatg gtctttgtcg attaactcaa
      121 tctcaccaat aaaacagggg gcggcaaaga aatcgatcag cgacacgcga ccattagcaa
      181 gcgtggcgta aagcctggcg cggcctcgcg tcagataaaa cagccaggac ggctgttgcc
      241 cttcctgcac aatgtagtca cgcgctaaaa aatgaaacag ccgcgtatcg gctgaaacat
      301 ccgttaaaaa acaatcctta aacgcagatt cactcatcaa ttgctgctta agatcattat
      361 tgtggatttc tttcacgatg tttatccgct atgagatttc tcatatttag catacgtacg
      421 cggttggtaa gattcaatca tcggcccggt cgaattttct ttgctgtaga aaaaataacg
      481 caactggaaa cagaggaaat aaacaatgga aaagagaaaa attattctgg attgtgatcc
      541 gggtcatgat gatgctattg caatgatgat ggcagcgaaa catccggcaa tagatttatt
      601 aggcatcact attgtagcgg gtaatcagac gcttgataaa acattaatta atggcctgaa
      661 tgtttgccag aaactggaga ttaatgttcc ggtttatgcg gggatgccgc aacccattat
      721 gcgtaaacaa atcgttgccg ataatattca cggtgaaacc ggactggatg gcccggtatt
      781 cgagccgctg acccgccagg cagaaagtac ccacgcggtg aaatacatca ttgataccct
      841 gatggcaagc gatggcgata tcactctggt gccggttggt ccgctttcaa atatcgcggt
      901 ggcaatgcgt atgcaacccg cgatcctgcc caaaatccgt gaaattgtgc taatgggcgg
      961 cgcttacggt acaggcaact tcacgccatc tgccgagttc aacatctttg ctgatccgga
     1021 agcagcccgc gtggtgttca cctccggcgt tccattagtg atgatgggcc tcgatctcac
     1081 caaccagacc gtttgcaccc cggacgtgat tgcacggatg gaaagggcag gcggccctgc
     1141 cggagagctg ttcagcgaca tcatgaactt cactctcaaa acgcagttcg aaaactacgg
     1201 cctggctggc ggcccggtgc acgacgccac ctgcatcggt tatctgatta accctgatgg
     1261 cattaaaacc caggagatgt acgtcgaagt ggacgtcaac agtggccctt gctatgggcg
     1321 taccgtctgc gacgagctgg gcgttcttgg caagcccgct aataccaaag tcggcatcac
     1381 tattgatacc gactggttct ggggcttagt cgaagagtgc gtgcgcggct acatcaaaac
     1441 ccattaagtg ttgataacaa gccgggtcag tatcgcaaga tccaagcccg gctatttctg
     1501 atgggcagaa agccgtcgcc caatatgaca tgaagagata ataactatgg atgtcatgag
     1561 aagtgttctg ggaatggtgg tattgctgac gattgcgttt ttgctgtcag taaacaagaa
     1621 gaagatcagc ctgcgtaccg ttggcgcggc gttagtgtta caggtcgtga ttggcgggat
     1681 tatgctttgg ttaccgccag ggcgttgggt cgctgaaaaa gtcgcttttg gcgtgcataa
     1741 agtgatggcg tacagcgacg cgggtagcgc atttatcttc ggttcgctgg tcgggccgaa
     1801 aatggacacg ctgtttgatg gcgcaggatt tatctttggt ttcagggtat taccggcaat
     1861 tatcttcgtc actgcactgg tgagtattct ctactacatc ggtgtgatgg ggattttaat
     1921 tcgcattctc ggcggtatat tccagaaagc attaaatatc agcaagattg agtcattcgt
     1981 cgcggtcacc accattttcc tcgggcaaaa cgaaattccg gcgatcgtca aaccctttat
     2041 cgatcgtctg aatcgcaatg aattatttac agcgatttgt agtggcatgg cctcgattgc
     2101 tggttcgaca atgattggtt acgccgccct gggcgtacct gtggaatatt tgctggcggc
     2161 atcgttaatg gcgatcccag gcgggatctt gtttgcccgc ctgttaagcc cggctacgga
     2221 atcttcgcag gtttccttta ataacctctc tttcaccgaa acaccgccaa aaagcattat
     2281 tgaagccgct gcgacagggg caatgaccgg gctgaaaatc gccgccggtg tagcgacagt
     2341 tgttatggca tttgtcgcca tcattgcgtt aattaatggt attatcggcg gcgttggcgg
     2401 ctggtttggt tttgcacatg cctcgctgga gtccatttta ggttacctgt tggccccatt
     2461 ggcgtgggtg atgggggttg actggagtga tgcaaatctt gccgggagtt tgattgggca
     2521 gaagctggcg atcaatgaat ttgtcgctta tctcaatttc tcgccatatc tgcaaacggg
     2581 tggcactctg gatgctaaaa ccgtggcgat tatttctttc gcgttgtgcg gtttcgctaa
     2641 ctttggttct atcggggtgg tggtgggggc gttttctgcg gttgcgccac accgtgcgcc
     2701 ggaaatcgcc caacttggtt tacgcgcgct ggcggcggcg acactttcta acctgatgag
     2761 tgctactatt gcaggattct ttattggttt agcgtagtgc gcaatttatg agccggataa
     2821 gatgcggtca agcgtcgaat ccggcaacat tgttacacca ttggcactaa tgaaagcgcg
     2881 ttatcggcag acagggtagg gttgtttaag ctgccgctgg cgcgcgaaat tgccgcgcag
     2941 gctaccgcaa aacgggcgct gtcgcggaaa ctgtatcctt caagaaagct atataccagg
     3001 cccgccataa aaccatcgtc agcaccaaaa ctgtctactg tcgtatgcgc tggcgcggtc
     3061 agcaaaaatt gttctccatc cttttcgctg caataaactg actcatcggg caaataaaca
     3121 aacagttgct gaacaccttg ctgatgcaat gcattcactg cggcattacg gtcagcatcg
     3181 ctggtgatcg cctgtcccca taaaatttcc agctccggta aagtgggttt cagggtgtga
     3241 atatgcgcca gccagtgttt gattttgccc gctttgaatt ctgaaacggt atcgacaaac
     3301 accgggattt catcagcgag ggtaaagacc cattccagcg cctcggctgt caggttgcaa
     3361 tcagccagta ccacgcccgc atgacgaagt aaatcgcgcg acccgttcag tagttgcggt
     3421 gtcaactgtt ccagcagatg ggtatcgtta atcgccagca cggtttgatc gtctcgattg
     3481 gcaattgcca gatacgtcga tgtgctttga ccatgcaaac gaacgcagcc ggagacattc
     3541 acgccggcac ggcgcgtttc ttccaggagc atttcgccat aaaagtcatc gccaatcact
     3601 gaaagcaaat ggacgtcacg gcctaacagc gccagattgt gggcgatgtt gcgtcccacg
     3661 ccgcccgccg agcaatgaat tgtaccggga tgagaagccg cttgcgggta acggatatcc
     3721 gccatcccgc gaatatccat attgattgtc cccaccacta cgcagtattc ctgctcggtg
     3781 agaatgtaac ctttgccttt aatccgccct ttgcgcatta aatccataat atgcgccgca
     3841 acgcgcgaac gactgatttg cagcatgtcc gcaatttcgt tctgctgaat cagcgggtta
     3901 cgccgtaaaa ttgcaaggat ctccttttcc cggttattca tcttcaggct accgcttttt
     3961 cagtttgttg cgctttcagc caggcgatct cttctgccca gatatccggg ttgatggttt
     4021 caaggatcag cggaatgccg tcgaaacggt cgtcctgcat gatccagcgg aacgcatcat
     4081 gaccgatatt gccttcaccg aggctatgat ggcggtcaac gcggctgcca aaggtgcttt
     4141 tcgcatcgtt aaggtgcatc ccgcgcagat acttaaagcc aacaatacgg gcaaaatccg
     4201 cgaacgtttt ctcgcattcg gctggagtac gcaaatcata cccggcagcg aaagcatggc
     4261 aggtatcaat gcagacgccg acacgggatt tatcttccac gccgtcgata atcgccgcga
     4321 gatgttcgaa tttaaaccct aagttactgc cctgaccggc ggtgttttca atcaccgctg
     4381 ttacaccttg agttttatcc agcgcaatgt tgatggattc ggcaatacgc gcaaggcaat
     4441 cctcttctga aatctgcatc agatggctgc cagggtggaa gttgagcaaa gaaagcccca
     4501 gttgttcgca acgctgcatt tcatctataa aggcatcgcg cgatttttcc agagcttcag
     4561 tgaccggatg tccgaggtta atcagatagc tgtcgtgggg aagaatttgc gccgatgtgt
     4621 agtgatattt ttcacaggcc gctttgaatt catcgatggt ttgcgtcgtg agcggtgcgg
     4681 cacgccactg acgttggttt ttggtgaaca aggcaaacgc ggttgcgtcg atttcggcgg
     4741 cgcgaattgc ggcatttgcc agaccgccag cagcactaac gtgcgctcca atgtatttca
     4801 tgcgaggact cctgttaaac ccgctggagg aaaacggtaa tgatagcggg ttaacaggag
     4861 caagatgtag tggtttatgc gatgacgctt tgaatcacat agttaatcgc accaccacca
     4921 acaatcagcc aggcaaacag taccagtgcc atcagcagag gtttcgcccc ggcttttttc
     4981 agcgcgctga cgtgagtggt cagacccagc gccgccatcg ccattgccag caggaaggta
     5041 tccagcgtta ccagcatgtt caccacgctc tgcggtaaca ggtggaacga gttaaagatg
     5101 gcaacgacga tgaacaagat ggcaaaccac ggaatagtga ttttgctttt ctcgccgctg
     5161 ttcgccccag acagctgttt aacacgcgcc gccagcagga tgaggaacgg agccagcatc
     5221 atcacgcgca gcattttgga aataactgct gcgttttccg catccgggct gatagcatga
     5281 cctgccgcca ccacctgcgc cacttcgtgc acagtagaac caatgtagat accgaaagtt
     5341 tccggactaa accattgaga catcagcgga tatatcgccg ggtagaggaa aatcgcgacg
     5401 gtcccgaaga taacaacggt tgcaacagcc acggttactt tactggcttc cgctttcact
     5461 accggctcag tcgccagtac cgcggcagca ccacagatac tgctaccggc accgatcaac
     5521 cagctggtgt gcttatccag accaaacact ttctgcccca ggaagcaagc cagcaggaag
     5581 gtactggaca gcgtcaacac gtcaatgatg atcccactga taccgacatc ggcaatttgc
     5641 gagaacgtca gacggaagcc ataaagaatg atacccagac gtaataaata ttgcttggca
     5701 aacagcacac caccgtcaca gcttttccag atgtgcggat agatggtgtt gcctaaaacc
     5761 atccccaaca agattgcgag ggtgagggca ctaaacccgg cacccgcaac cgcgggaatg
     5821 gaaccacccc acagggcgac cccggtgata actgcactca gggctaaccc cggaataaaa
     5881 tgccacagtg tacgatgttg tttctgtaag gtgatattcg tcataaccct ctcctttaac
     5941 c
//
LOCUS       DAPHBJ010000236         5914 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.238, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000236 DAPHBJ010000000
VERSION     DAPHBJ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.238"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            57..1208
                     /gene="yiaY"
                     /locus_tag="RHZ76_004115"
     CDS             57..1208
                     /gene="yiaY"
                     /locus_tag="RHZ76_004115"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HDV2814646.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1373..2911
                     /gene="aldB"
                     /locus_tag="RHZ76_004116"
     CDS             1373..2911
                     /gene="aldB"
                     /locus_tag="RHZ76_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HDV2814647.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3130..3285)
                     /locus_tag="RHZ76_004117"
     CDS             complement(3130..3285)
                     /locus_tag="RHZ76_004117"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814648.1"
                     /translation="MAKLMNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRP
                     LTIETRD"
     gene            3456..3779
                     /locus_tag="RHZ76_004118"
     CDS             3456..3779
                     /locus_tag="RHZ76_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDV2814649.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQSHDSSVMQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            3785..4921
                     /locus_tag="RHZ76_004119"
     CDS             3785..4921
                     /locus_tag="RHZ76_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV2814650.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(4918..5892)
                     /locus_tag="RHZ76_004120"
     CDS             complement(4918..5892)
                     /locus_tag="RHZ76_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2814651.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANASKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQQPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         1465 a         1410 c         1542 g         1497 t
ORIGIN      
        1 cgaatgaaaa tcctcagtaa gctgcccgcc cttttttaca ctttcaggag tgtgttatgg
       61 catcttcaac tttctttatt ccttctgtga atgtcatcgg cgctgattca ttgactgatg
      121 caatgaatat gatggcagat tatggattta cccgtacctt aattgtcact gacaatatgt
      181 taacgaaatt aggtatggcg ggtgatgtgc aaaaagcact ggaagaacgc aatattttta
      241 gcgttattta tgatggcacc caacctaacc caaccacgga aaacgtcgcc gcaggtttga
      301 aattacttaa agaaaataat tgcgatagcg tgatttcctt aggcggtggt tctccgcatg
      361 actgtgcaaa aggtattgcg ctggtggcag ccaatggtgg tgatatccgt gattatgaag
      421 gcgttgaccg ctctgcaaaa ccgcagctgc cgatgatcgc catcaatacc actgcgggta
      481 cagcatcaga aatgactcgt ttctgcatca tcaccgacga agcgcgtcac atcaaaatgg
      541 cgattgttga taagcacgtg actccgctgc tttctgtcaa tgactcctcg ctgatgatcg
      601 gtatgccgaa gtcactgacc gccgccactg gtatggacgc cttaacgcac gctatcgaag
      661 cgtatgtttc tattgccgcc acgccgatca ctgacgcttg tgcactgaaa gccgtgacca
      721 tgattgccga aaacctgccg ttagccgttg aagatggcag taatgcgaaa gcgcgtgaag
      781 caatggctta tgcccagttc ctcgccggta tggcgttcaa taatgcttct ctgggttatg
      841 ttcatgcgat ggcgcaccag ctgggcggtt tctacaacct gccacacggt gtatgtaacg
      901 ccgttttgct gccgcatgtt caggtattca acagcaaagt cgccgccgca cgtctgcgtg
      961 actgtgccgc tgcaatgggc gtgaacgtga caggtaaaaa cgatgcggaa ggtgctgaag
     1021 cctgcattaa cgccatccgt gaactggcga agaaagtgga tatcccggca ggcctacgcg
     1081 acctgaacgt gaaagaagaa gatttcgcgg ttctggcgac taatgccctg aaagatgcct
     1141 gtggttttac taacccgatc caggcaactc acgaagaaat tgtggcgatt tatcgcgcag
     1201 cgatgtaatc atcatttcca caacggctgg caaattgtca gccgcttttt caactatctc
     1261 tgtaaccctt gcccgtaaat tcgtgatagc tgtcgtaaag ctgttaccga ctggcgaaga
     1321 tttcgccagt cacgtctacc cttgttatac ctcacaccgc aaggagacga tcatgaccaa
     1381 taatccccct tcagcacaga ttaagcccgg cgagtatggt ttccccctca agttaaaagc
     1441 ccgctatgac aactttattg gcggcgaatg ggtagcccct gccgacggcg agtattacca
     1501 gaatctgacg ccggtgaccg ggcagctgct gtgcgaagtg gcgtcttcgg gcaaacgaga
     1561 catcgatctg gcgctggatg ctgcgcacaa agtgaaagat aaatgggcgc acacctcggt
     1621 gcaggatcgt gcggcgattc tgtttaagat tgccgatcga atggaacaaa acctcgagct
     1681 gttagcgaca gctgaaacct gggataacgg caaacccatt cgcgaaacca gtgctgctga
     1741 tgtaccgctg gcgattgacc atttccgcta tttcgcctcg tgtatccggg cacaggaagg
     1801 cggtatcagt gaagttgata gcgaaaccgt ggcctatcat ttccacgaac cgttaggcgt
     1861 ggtggggcag attattccgt ggaacttccc gctgctgatg gcgagctgga aaatggctcc
     1921 cgcgctggcg gcgggcaact gtgtggtgct gaaacccgca cgtcttaccc cgctttctgt
     1981 actgctgcta atggaaatcg tcggtgattt actgccgccg ggcgtggtga acgtggtcaa
     2041 cggcgcaggt ggggaaattg gcgaatatct ggcgacctcg aaacgcatcg ccaaagtggc
     2101 gtttaccggc tcaacggaag tgggccaaca aattatgcaa tacgccacgc aaaacattat
     2161 tccggtgacg ctggagctgg gcggcaaatc gccaaatatc ttctttgctg atgtgatgga
     2221 tgaagaagat gcctttttcg ataaagcgct ggaaggcttt gcactgtttg cctttaacca
     2281 gggcgaagtt tgcacctgtc cgagtcgtgc tttagtgcag gaatctatct acgaacgctt
     2341 tatggaacgc gccatccgcc gtgtcgaaag cattcgtagc ggtaacccgc tcgacagcgt
     2401 gacgcaaatg ggcgcgcagg tttctcacgg gcaactggaa accatcctca actacattga
     2461 tatcggtaaa aaagagggcg ctgacgtgct cacaggcggg cggcgcaagc tgctggaagg
     2521 tgaactgaaa gacggctact acctcgaacc gacgattctg tttggtcaga acaatatgcg
     2581 ggtgttccag gaggagattt ttggcccggt gctggcggtg accaccttca aaacgatgga
     2641 agaagcgctg gagctggcga acgatacgca atatggcctg ggcgcgggcg tctggagccg
     2701 caacggtaat ctggcctata agatggggcg cggcatacag gctgggcgcg tgtggaccaa
     2761 ctgttatcac gcttacccgg cacatgcggc gtttggtggc tacaaacaat caggtatcgg
     2821 tcgcgaaacc cacaagatga tgctggagca ttaccagcaa accaagtgcc tgctggtgag
     2881 ctactcggat aaaccgttgg ggctgttctg atataagaag ctggtcgcat tgggtattca
     2941 ttgcctgatg cgacgcttac gcgtcttatc atgcctacgg gaacctgacc gtaggccgga
     3001 taaggcgtac acgccgcatc cggcataaac aaagcgcacg ttgttacaat ttacgtttta
     3061 aacctacgtg cgcaataacc cagactacgc caacagaagt acgtttctgg ggcgtgtttt
     3121 gtcaggagtt taatctcgcg tttcgatggt gagcgggcga accaggccgt aaatcaccat
     3181 tggtgatccg aggccaatca gcaataaacg gtagataatt gaaggtttaa tacgcttaat
     3241 ctgtactgtg gatgttttac tggagttatt cattagtttg gccaatagtt ccactatttc
     3301 aatatgataa acctcgtcct gttgaaaaac cagacagcac atttaagaaa gtatataaaa
     3361 aacaatatgt cgttatataa ataaggaaat tgcactgcat catggcattt cataatatgc
     3421 gttaattgtt cttttttata ttctttgagg gaaatatgtt cctggactat tttgcactgg
     3481 gagtgcttat ttttgtattt ctggtgatct tctatgggat cattatttta catgatattc
     3541 cctacctgat tgccaaaaaa cgtaatcatc ctcatgccga cgccattcat gttgctggtt
     3601 gggtgagtct ttttacgtta catgttatct ggccgttttt gtggatttgg gccacgcttt
     3661 accgcccgga gcggggatgg ggaatgcaaa gccatgattc atccgttatg caactgcaac
     3721 agcgcattgc cgggctggag aaacagctcg ccgacatcaa atcctcttct gccgagtaat
     3781 acttatggat ttactgatta ttttgaccta tgtggctttt gcatgggcaa tgtttaagat
     3841 cttcaaaatt cccgtaaata aatggaccat tcccacagcg gccctgggtg gaatatttat
     3901 tgtcagcggt ctaattctgt taatgaacta taaccatccg tataccttta aagcgcaaaa
     3961 agcggttatt tctattcctg ttgtcccaca ggtgactggt gtggtgattg aagtgacgga
     4021 taagaaaaat acgctgatta aaaaaggtga ggtgctattt cgactggacc cgacgcgtta
     4081 tcaggcgcgg gtggatcggc tgatggcgga tatcgttacc gcagaacata aacagcgggc
     4141 gttgggcgca gagttagatg agatggcggc gaatactcag caggcaaagg ccacgcggga
     4201 taaattcgct aaagagtatc agcgttacgc acgtggcagc caggcgaaag taaacccgtt
     4261 ttcagaacgc gatatcgatg tggcgcggca aaattatctg gcgcaggaag cctccgtgaa
     4321 gtcatcggcg gcggaacaaa aacagatcca gagccagctg gatagcctgg tgttgggtga
     4381 acattctcaa atcgccagcc tgaaagcaca gctcgcggaa gcaaaatata accttgagca
     4441 gacgatagtg cgtgcgccga gcgatggtta tgtgacccag gtactgattc gtccgggtac
     4501 ctatgccgcg tcgctgccgc tacgtccggt gatggtgttt atacccgatc agaaacgaca
     4561 aatcgtggcg cagttccgtc agaactcctt gctgcgactg gctcctggcg acgatgcgga
     4621 agtggtgttt aatgctctgc caggtaaggt attcagcggt aagctggcag ccattagtcc
     4681 agccgttccc ggcggagctt atcagtcgac cggcacctta cagacgttaa acacagcgcc
     4741 gggttcagat ggcgttatcg cgaccattga actggatgag cacactgatt tgagcgcttt
     4801 accagacggt atttacgccc aggtggcggt ttactctgat catttcagcc atgtctcggt
     4861 gatgcgcaaa gtgctattac gcatgaccag ctgggtgcat tacctttatc tcgatcatta
     4921 atcttcgctt tccgccgctt gttcaggccg cagttgctgc gactgagcaa gctggtggcg
     4981 aatttcacgc agcatttcat ccagcacata ctggacccgc cagggctggt attcccgttt
     5041 acggaagata gccaccagat ccagccacca ttcatagggt tgctgaaagc agggcaccag
     5101 cgtgccgtct tgcaggtcgc tctgcacact tttgtctggc gcaaacacaa tccccaggtg
     5161 atttcgtgcc agctccagcg cagattgcgt gttgtcgcaa acgtaatttc ccttcacgcg
     5221 ataatcccgc acctctttac tcccggcgac gttgaagcgc cagatattcg cgtcgtcgat
     5281 catcatcgag tcgatcaaaa tacaggagtg atgttcgagt tcgtcaggac ggctaatggg
     5341 atgtttctca agatagcgct ggctggcata agcggttacg gcatattggg taataaaact
     5401 ggcaaccagc gattcatctt ttggtggagc gtagctgatt aaaacatcac aatcatcagg
     5461 aaattcgacg ccttcataaa aggcattacg ctcaagattg caggttttta gcgataaggt
     5521 gatatcaccg atatctttaa ttttatcgat tacatgtttg gataaatagg taattatgcc
     5581 ggttggggcg tagatggtga cccgaccacg tttctcatgc ttataatctg caataaaatt
     5641 attaagctgc tcgtttctgt ccagcatgtc gttgatgtac ggtaacagcg cggtaccaaa
     5701 aggtgtgagc atcagctgcc gggtggttcg gtcaaagact tttaaaccca cttttgattc
     5761 aaaatcagca agatatttgc tggcgttggc ctgtgcgatg ccaagtacag tcgcggcatg
     5821 gctgatattt tcactggcag cgattaccga gataattttt aactcccggt acttaagttg
     5881 taattttgtc atcgcaacag cccaattata tata
//
LOCUS       DAPHBJ010000237         5904 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.239, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000237 DAPHBJ010000000
VERSION     DAPHBJ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.239"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1040
                     /locus_tag="RHZ76_004121"
     CDS             <1..1040
                     /locus_tag="RHZ76_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HDV2814652.1"
                     /translation="SSAIIVAIMSLGLTGCDDKKAETETLPPANSQPAAPAPEAKPTE
                     APVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYADWLKDFKQ
                     GPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTI
                     NEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAELK
                     NIEEREGKTFHYYSLAVMISAKQINNLISQDKFDAEAAMKKVSELETLVAQAKEADKG
                     GMNFSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPRALREY
                     NEMVDDYNSLR"
     gene            complement(1090..2838)
                     /locus_tag="RHZ76_004122"
     CDS             complement(1090..2838)
                     /locus_tag="RHZ76_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HDV2814653.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNAVRTLTGLIEKQHQQAQVISADNVQGLLQRVPGIASLNIIDAQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(2838..3908)
                     /locus_tag="RHZ76_004123"
     CDS             complement(2838..3908)
                     /locus_tag="RHZ76_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDV2814654.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEVEKRGVEIGNFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYEALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            complement(3898..5349)
                     /locus_tag="RHZ76_004124"
     CDS             complement(3898..5349)
                     /locus_tag="RHZ76_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HDV2814655.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            complement(5360..5806)
                     /locus_tag="RHZ76_004125"
     CDS             complement(5360..5806)
                     /locus_tag="RHZ76_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDV2814656.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS"
BASE COUNT         1527 a         1524 c         1498 g         1355 t
ORIGIN      
        1 tatcttccgc tattatagtc gccatcatgt ccctcggtct gacgggttgt gatgataaaa
       61 aagccgaaac agaaacgctc ccgcctgcca atagtcaacc tgccgcacca gctcctgaag
      121 cgaaacctac tgaagctccc gttgcaaaag cagaagctaa acctgaaaca cctgcgcaac
      181 cggtggtcga tgaacaagcg gttttcgacg aaaaaatgga tgtctatatc aagtgctaca
      241 acaagttaca gatcccggta cagcgcagtc tggcgcgtta tgctgactgg ctgaaagatt
      301 ttaaacaggg gcctaccggt gaagagcgta ctgtttatgg catctacggc attagtgaat
      361 ccaacctcgc tgagtgtgaa aaaggcgtaa aaagtgctgt ggcgttaacg cctgcgctgc
      421 aaccaattga tggcgtggcg gtgagttata tcgatgctgc cgtggcgttg ggtaatacca
      481 ttaacgaaat ggataaatat tacacccagg aaaattataa agacgatgcg tttgcgaaag
      541 ggaaaacgct gcaccagaca ttcttaaaaa atctggaagc ctttgaacct gtagccgaat
      601 cttatcatgc ggcgattcag gaaattaatg ataagcgtca gcttgcggaa ctgaaaaata
      661 ttgaagaaag agaagggaaa acattccact actactctct ggcagtcatg atttcagcga
      721 aacaaattaa taacctgata tcgcaagata agtttgatgc agaagcggca atgaagaaag
      781 tgtctgaact ggaaacgctg gtggcgcagg ccaaagaagc ggataaaggc ggcatgaatt
      841 tctcgtttat taattcggca ggccagtatc aactcgaggc taaaaaatac gttcgccgca
      901 tcagagataa agtcccgtac tctgactggg ataaagagca acttcaggac gcaaactcaa
      961 gctggatggt cgaagactct ttcccgagag cattacgcga gtacaacgaa atggttgatg
     1021 actataatag cctgcgttaa acgtttttga tgataaatgc agtgtcggat gcggcgcatc
     1081 cgacacagtt caccccttgt gaagtaactc cataaatgcc tcatatcctg ttaacccggc
     1141 aataacctct gccggatgct gacacaatac gcttgccaga tagctgaata ttttcaactc
     1201 gtgacgcgca tcggcggcgg cgcaggcgat caagacatgg ttaatgactt cgttattcac
     1261 ctcaactggt tgggcgaggg taataaaaaa tccacgaaaa cgtcgctcct gttcgctcca
     1321 gcaatgtggg atggcgaggc gattaacaat caggttttca ccttcgcctt cgcgggcgat
     1381 gatccgttgc gcttcatcgg cggtaatatg gtgttgtgcc actaattgcg cacaaatttg
     1441 ccgggtaata tgttgccagc tttcaccgcg tacattcgaa taatgaaaac ttccgggggc
     1501 agagaaaaaa cgctccggtt gctggcgaat aaatgccgtc gccagaaaat gttttatttg
     1561 ttcgataccg gcagccgtaa tgatattttt tacggtgatg taattattga ccgcgtcatc
     1621 cagtaaataa tggctgttgt taatgatcaa taacggctca atctcttcgc gaatggcaac
     1681 aagttcgctt aagctacggg caataatcac ccgacaatgt aaaacatcgc gctcaatggc
     1741 gagctgatta atggtggcaa tcgcgttctg gtccgagagc aaaatgatcg gctggcgttc
     1801 gttttgatgc cgttccagcg cacaggcaaa atacaaaccg atcagatcgc tgtcgaaaag
     1861 aggaatatcg agctgttcac gcagtagcgt aataaagtgc agactcatat caaaggccgc
     1921 aggccaggcg gctttcaggt tattcatgct gctctggcgg tgctcagcaa tccacactgg
     1981 tgcggcaagg caacgtaata aatgcccggt aatattctca accagctgcg catcaataat
     2041 attcaatgac gcgatgcccg gcaccctttg cagcaacccc tgcacattat cggctgaaat
     2101 tacctgcgct tgctgatgct gtttctctat caaacccgtc agggttctta cagcgttatc
     2161 accaagaaac agaccgctat gttcaacgat ttgttttatc tcgtcaccgg gatattgcgg
     2221 ccactcatcg gtcagttgat tacgcagggc gtaaatcgcc agaatcaggc tggagagata
     2281 atcaccctgc atgagcggcc agcggtgttg gttgtcgcag gccgtggata aatgctgaag
     2341 gttgtctcgt gttatgcccg ccagcggaat taaaaacgga tctttgcgca gcaagttcgc
     2401 cagcaagata acgcgtttct cttctgtctc atcaatgaaa tggcccaaac cagggcggct
     2461 ggccaggcaa caagtgcgtt cataacgctg ttttaaccgg ggcagacgct ctgctaccca
     2521 cgtttctggc aaattaagcg ccgaggcgag ttgcgcacgg ggagtgaaag tattcagtaa
     2581 taacagcgcc agcagccgat cgtcgttatc gtgcttttgc agcaactgaa aaaagctgcg
     2641 gcgctcgaag atttccagct gatagcccgc actgccactg gcgaaaatgc gggctttgcc
     2701 gttaagggtg aagttgagat agtcaatatc acgcaggatg gtcctgcctg aaacgccagt
     2761 ctgttgcgcc agctcgccag gcgtgcgcgg ctgttgctcc agcagatcga caatctttaa
     2821 ctggcgttcg tttagcatta atccacctga cggaactggc taaacgcgtt gactttctcc
     2881 gcagttaagg tcgtcagaaa accccgtatc agcgtgagca gagcttcgta atcggctttt
     2941 gaaatcatac cgctgttggc gtgcagataa cgagttggca gacacagcgc gacaaccgga
     3001 cgcccgccgc ccatcacgtt gtagcgcccg ccatccgtcg caccggtttt catggtggaa
     3061 aattgcagcg gtaaatcgtt atgtgcggca cagcttttta acgctgctac cagtttctgg
     3121 ttggggaagt agcgcttgtc aaacagcatc agccccggcc cctggcccag tttcagcggg
     3181 tatttaatgt tatcaatgcc cggaacatcg cccgctacgg cggtatccaa cacgatcacg
     3241 acgtccggtt taatgtgttc cgccgaggtt tgcgccccgc gtagcccaac ttcttcttcc
     3301 acactgccaa cgccatacag cgtaatttcg ggattattca ccgtctgcaa tagttcagcc
     3361 atcattgcgc agccgatgcg gttatccaac gccttgccga ccactttatc ttcgccccag
     3421 caggcaaaat tggcttccgg gctaataaaa ttaccaattt ccacgccgcg cttttccacc
     3481 tcttcgcgac tgttcgcgcc aatatcaatg aacatctcat caaatgacag cggttgttgc
     3541 ttttgctttt ccgttaacgc atgaggcgcg acggaaccaa tcacaccagg gattttcact
     3601 cccttgtgtg tgcgtatggt tacccggtgg ttgagcatcg actgattcca ccagccgcca
     3661 atggtggtaa aacgcagaaa accgctctcg tcgatgtggg tgaccataaa gccgacttca
     3721 tccatatgtc cgacaacggc aacttttgga cctttattcc ccttacgggc aacaaagctg
     3781 cccagaccat cgaaggtgat ttcattcacg caaggttcca gcgtgtttat cagaatgtcg
     3841 cgaacttcct gttcatcgcc gctgacggcg ctggcttcgc acaactgttg cagtaactca
     3901 atgttcatgg acaagctcct gttgcgcggc tttcgctttg cggcgtaacc acaatccttt
     3961 caggacgata atgaatgaaa tgttcagcgc gaggccaatc gccagcacca gataaaacga
     4021 acccaccggc gacatcaggc cgatcagcgg gtcaaagata cccaaaccag gtgccagacg
     4081 tttgattccg aaagcgatca ccagcacgcc ggtaatgccg cccgccagcg tattggcggt
     4141 aatcatcggc aacggagctg ccagagcgta aggaatagcc ggttcggttg cgactgtggc
     4201 accgaccaca atggcactac tggcagcctc tttttcctgt cgggtaaaca gtttgggcgc
     4261 aatgaacgtc gcaagacccg ccgcgacggg cggcatcagc gcgacaacgc cgacgatggc
     4321 gtaccagtca taaatgtgtt tttccagcag tgagaagcag aagaaccagg cggttttatt
     4381 gatggggccg cccatatcga acgccagcat cgcaccgacc agaaacgctg ccgcaaactt
     4441 cattgatgga ggaatggtat tcaggaagtg gagcagcccg cccatgaggt cggacatcac
     4501 cggcccgata acgtagtaag tgagcacgcc gaaaaccagc agagtaacga acgggatcag
     4561 cattgaaccg agcagcggtt gtaatgcttt gccaaggcgc actttgcgga accagaagac
     4621 gaaatagcca atcgccagcc ccagcaccac cgcgccgagg aatcccgcac ccgactgggt
     4681 gccgagcagc gctttgtcgt tcgccagata gcaaaccaga aacgccggag cgaaagccgg
     4741 tttatcggca atcgacgagg caatataagc gcccatgatg gggatcataa aggtgaagcc
     4801 aaggtaaccg atggattcaa ctacccaggt aaacgacggc gcgcctttcg acatatcggt
     4861 atacggcaaa ccgaactgca ccagcatgtt ggcaagcgcc accaggatac caccgccgat
     4921 gacaaacggc agcgccgcag agacgcccgc catcaggtgg ctcatcacgc taccgctttg
     4981 cacttcctgt ttgccaagct tcacgccgct atctgcggca aaaagctgcg agtttgtcgg
     5041 taattcgctg aaaatctggt cgatattttt caacgcctgg gagatggcaa tctcataaac
     5101 tttcttcccg gcaaaccgcg cgcgatcatc accgctcagg ccacgcccgg tagcgagaat
     5161 gacgtaatcg gcagcggcaa tctcttcgct ggataagcgg ttttcaacgc cactggaccc
     5221 ttgagtttcc acttttatgg tatgaccgag agaacgggct ttctgttcca gggcttccgc
     5281 caccatgtag gtgtgagcga tcccggcggg gcagttggtg atcgcgacaa tacgtaagga
     5341 tgactccatc gccacttcct tatgagctga gggtttgatt taacaaggca agcacctggt
     5401 cggtatcgcc ctgttgcagt tgatgaataa attccttgtg aataattttg cgacacagtg
     5461 tgccgatgat tttgacctga tcctcttcgc cgctttgcgg cacgccgagg cagatccagc
     5521 aattgacgtc ttcgccatcg ctggctttcc agtcaatagc ctgcgctttg cgggcgaata
     5581 atacgaacgg ttgtttaacg caggcgcttt taccgtgcgg cacggcgaca ccggaaccaa
     5641 atccagtaga gtgcattttt tcgcgcagca gcagggtttg caggaactgg tgtgagtcgg
     5701 tgataaaacc gttttgtaac gctattgtcg ccaactgctt cagaacggaa taagcgccat
     5761 tgccctgaat attcaggtca atacagcttg cagtaagagc tgccattaat cggactccat
     5821 aaaatatatt ttaatcaatt gtaatggtgg agcgattaag tgatgagagg cgatatgtcg
     5881 gtaaacaatg acaatttgag acag
//
LOCUS       DAPHBJ010000238         5891 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.240, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000238 DAPHBJ010000000
VERSION     DAPHBJ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.240"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            236..4492
                     /gene="fdeC"
                     /locus_tag="RHZ76_004126"
                     /pseudo
     CDS             236..4492
                     /gene="fdeC"
                     /locus_tag="RHZ76_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001635811.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
     gene            complement(4632..5483)
                     /locus_tag="RHZ76_004127"
     CDS             complement(4632..5483)
                     /locus_tag="RHZ76_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV2814657.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLINFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCEYADMPKEWTTTRGLY
                     ASFRYEGNWENYPDWVRNIYLIELPARGLARVNGSDIERFYYNEDFVEKDGNDVVCEI
                     FIPVRPV"
     gene            5786..5891
                     /locus_tag="RHZ76_004128"
                     /pseudo
     CDS             5786..5891
                     /locus_tag="RHZ76_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
BASE COUNT         1588 a         1336 c         1558 g         1409 t
ORIGIN      
        1 gacttgcaca aaaagctaaa ttttccccca taaataaaaa tataatcccg cgcccaacca
       61 cctgatgagt ggctacaggc actggatata ttaggtggcg gtgcactttc ttacataaag
      121 gtatttcctt ttctgcggaa aaggaaatcg ggaaatcccc ggtttttctg acaagcagac
      181 gccattattt gtgtctgcct atgttcgtta attcgttcat caggaaatta tctcaatgtc
      241 acattataaa acaggtcata aacaaccacg atttcgttat tcagttctgg cccgctgcgt
      301 ggcgtgggca aatatctctg ttcaggttct ttttccactc gctgtcacct ttaccccagt
      361 aatggcggca cgtgcgcagc atgcggttca gccacggttg agcatgggaa atactacggt
      421 aactgctgat aataacgtgg agaaaaatgt cgcgtcgttt gccgcaaatg ccgggacatt
      481 tttaagcagt cagccagata gcgatgcgac acgtaacttt attaccggaa tggccacagc
      541 taaagctaac caggaaatac aggagtggct cgggaaatat ggtacagcgc gcgtcaaact
      601 gaatgtcgat aaagatttct cgctgaagga ttcttcgctg gaaatgcttt atccgattta
      661 tgatacgccg acaaatatgt tgttcactca gggggcaata catcgtacag acgatcgtac
      721 tcagtcaaat attggttttg gctggcgtca tttttcagga aatgactgga tggcgggggt
      781 gaatactttt atcgatcatg atttatcccg tagtcatacc cgcattggtg ttggtgcgga
      841 atactggcgc gattatctga aactgagcgc caatggttat attcgggctt ctggctggaa
      901 aaaatcgccg gatattgagg attatcagga acgcccggcg aatggttggg atatccgcgc
      961 agagggctat ttacctgcct ggccgcagct tggcgcaagc ctgatgtatg aacagtatta
     1021 tggcgatgaa gtcgggctgt ttggtaaaga taagcgccag aaagacccgc atgctatttc
     1081 tgccgaggtg acctatacgc cagtgcctct tctgacactg agcgccgggc ataagcaggg
     1141 caagagcggt gagaatgaca ctcgctttgg cctggaagtt aactaccgga ttggcgaacc
     1201 tctggagaaa caactcgata cagacagcat tcgcgagcgt cgcatgctgg caggcagccg
     1261 ctatgacctg gttgagcgta ataacaacat cgttcttgag taccgcaaat ctgaagtgat
     1321 ccgtattgct ctgcctgagc gtattgaagg caagggcggt cagacacttt ccctcgggct
     1381 tgtggtcagc aaagcaactc acggactgaa aaatgtgcag tgggaagcgc cgtcattact
     1441 ggctgaaggt ggcaaaatta ccggtcaggg tagtcagtgg caagtaacgc tcccggctta
     1501 tcgtccaggc aaagacaatt attatgcgat ttctgcggtt gcctacgata acaaaggcaa
     1561 tacctcaaaa cgcgtgcaga cagaggtggt cattaccgga gctggtatga gcgccgatcg
     1621 cacggcgtta acgcttgacg gtcagagccg tattcaaatg cttgctaacg gtaatgagca
     1681 aaaaccgctg gtgctgtctc tgcgcgacgc cgagggccag ccagtcacgg gcatgaaaga
     1741 tcagatcaag actgaactaa ctttcaaacc ggctggaaat attgtgactc gtaccctgaa
     1801 ggccactaaa tcacaggcaa agccaacact gggtgagttc accgaaactg aagccggggt
     1861 gtatcagtct gtctttacta ccggaacgca gtcaggtgag gcaacgatta ctgttagcgt
     1921 tgatggcatg agcaaaaccg tcactgcaga actgcgggcc acgatgatgg atgtggcaaa
     1981 ctccaccctg agcgctaacg agccgtcagg tgacgtggtt gctgatggtc agcaagccta
     2041 tacgttgacg ttgactgcgg tggactccga gggtaatccg gtgacgggag aagccagccg
     2101 cttgcgattt gttccgcaag acactaatgg tgtaaccgtt ggtgccattt cggaaataaa
     2161 accaggcgtt tacagcgcca cggtttcttc gacccgtgcc ggaaacgttg ttgtgcgtgc
     2221 tttcagcgag cagtatcagc tggacacatt acaacaaacg ctgaagtttg ttgccgggcc
     2281 gcttgatgca gcacattcgt ccatcaccct gaatcctgat aaaccggtgg ttggcggtac
     2341 agttacggca atctggacgg caaaagatgc taatgacaac cctgtaactg gtctcaatcc
     2401 ggatgcaccg tcattatcgg gcgcagctgc tgtaggttct acggcatcag gctggacgga
     2461 taatggcgac gggacctaga ctgcgcagat ttctctcggc actacggcgg gtgaattaga
     2521 ggttatgccg aagctaaatg gacaggatgc ggcagcgaat gcggctaaag taaccgtggt
     2581 ggctgatgcg ttatcttcag gccagtcgac agtgtctgta gccgcagatc gcgtaaaagc
     2641 aggcgaaagc acaaccgtga cgcttattgc aaaagatgca catggcaacg ctatcagtgg
     2701 tctttccctg tcggcaagcc tgacgggtgc tgcgtctgaa ggggcgactg tttctggttg
     2761 gaccgaaaaa ggtgatggtt cctatgtcgc tacgctgaca acaggtggaa agacgggtga
     2821 gcttctcgtc atgccgctct tcaacggcca gccagcagcc accgaagccg cgcagttgac
     2881 ggtcatcgcc ggagagatgt catcagcgaa ctctacgctt gttgcggaca ataaggctcc
     2941 gaccgtcaaa acgacgacgg aactcacctt cactgtgaag gatgcgtacg ggaacccggt
     3001 caccgggctg aagccagatg caccagtgtt tagcggtgcc gccagcacgg ggagtgagcg
     3061 tccttcagca ggaaactgga cagagaaagg taatggggtc tacgtgtcga ccttaacgct
     3121 gggatctgcc gcgggtcagt tgtctgtgat gccgcgagtg aacggccaaa atgccgttgc
     3181 tcagccactg gtgctgaatg ttgcaggtga cgcatctaag gctgagattc gtgatatgac
     3241 agtgaaggtt aataaccaac tggctaatgg acagtctgct aaccagataa ccctgaccgt
     3301 tgtggacagc tatggtaacc cgttgcaggg gcaagaagtt acgctgactt taccgcaggg
     3361 tgtgaccagc aagacgggga atacagtaac aaccaatgcg gcagggaaag tggacattga
     3421 gcttatgtca acggttgcgg gggaacacag catcacggcc tcagtgaata atgctcagaa
     3481 gacggttacg gtgaaattca aggcggattt cagtaccggt caggcgaccc tggaggttga
     3541 tggcagcacg ccaaaagtgg caaacgacaa tgatgccttt acgctgacgg caacggttaa
     3601 ggatcaatac ggcaaccttc tgcctggcgc tgtggtcgtc tttaatctgc ctcggggcgt
     3661 caaaccgctt gcagacggta atatcatggt gaacgccgac aaggagggta aagcggaact
     3721 gaaagtggtc tccgtgactg ccggaacgta tgagatcacg gcgtcggcag gaaatgacca
     3781 gccttcgaat gcgcagtctg taacgtttgt ggccgataag actacggcga ccatctccag
     3841 tattgaggtg attggcaacc gtgcagtggc ggatggcaaa accaaacaga cgtataaagt
     3901 tacggtgact gatgccaata acaacctgtt gaaggatagc gacgtgacgc tgactgccag
     3961 ctcggaaaat ttagttctgg atcctaaagg gacggcgaaa actaatgagc aaggacaggc
     4021 tgttttcacc ggctctacca ctatcgcagc gacatataca ctcacggcga aagtggaaca
     4081 ggccaacggt caggtatcga cgaaaactgc tgaatctaaa ttcgtcgcgg atgataaaaa
     4141 cgcggtgctc gccgcatctc cagaacgtgt agattctctg gtggcggacg ggaagactac
     4201 tgcaacaatg acggttaccc tgatggcggg agtcaatccc gtaggaggaa gtatgtgggt
     4261 cgacattgag gctccggaag gagtgacgga gaaggattat caattcctgc cgtcgaaggc
     4321 tgaccatttc tcaggtggga aaatcacgcg tacatttagt accagcaagc caggtgtcta
     4381 tacgttcaca ttcaacgcac tgacgtatgg cgggtacgaa atgacgcctg tgaaggtgac
     4441 aattaacgcc gttgctgcag agactgaaaa tggcgaggag gagatgccat aatttgaata
     4501 gtatgaaagt tacgatctaa taaagaataa tggtgttgaa atattcaacg ccattattca
     4561 ctgaatacct tgcctgatac tgattaaata gtatcaggca aggataaaac tcaataagag
     4621 atagtgacca actaaaccgg acgaacggga ataaaaattt cgcaaacaac atcattgcca
     4681 tccttttcta cgaaatcttc attgtaataa aagcgctcaa tatcgctgcc gttcactctg
     4741 gctaaccccc tggcaggtaa ctctatcaga tagatgttac gcacccagtc aggataattt
     4801 tcccagtttc cttcataacg gaaagaggca taaagtcctc gggtcgttgt ccactctttt
     4861 ggcatgtcag catattcaca taccgtacca gcaaaagttt caaccgtgag caaatcgacc
     4921 gattttgttg acggttcaaa cgtggaaacg aaatagatat ccgtcttaaa attcttcata
     4981 catctggcta aatgatgacg tcgtagtttg acttcttcat ccggtgattt attcgacact
     5041 tcaatgtcat atttaaatga gttgccaaaa ataggtgtct caggaaagtt gattaatcta
     5101 cactccgtct tttgctgttg acgtattaaa aaagaaggga agatatttgc gagatcccaa
     5161 taatcacggt ggcggtattc acggggagag cagccgaata actttttaaa ttcgcggctg
     5221 aatgactgct gtgaatcaaa atgcaaagag agtgcaatat caagcataga tttcgcggtg
     5281 agccggacaa gaatggcggc acgacaaagc cttcgtttgc gaatgtattc tcccaaaggc
     5341 acatgcatga aatttcggaa cagaagctgg atgttgcgtc tgctgtagcc agatttctgt
     5401 gcgatatctt cgattgaaat agggtgctca agattgcact caatccactc caggagctgc
     5461 tgtagaatct tctgcctgat catctcattc tccgttaatt ctttgtcata tcgcacattt
     5521 tcgttatacc ctgggcccta agtgtatcaa aaatagcaag ggcgaaggca aaagcactgg
     5581 tagcgtctgc ttcgttgtcc ccgaaaatag agaggtttca gtcctacatt attaatgaat
     5641 tttttgcata agtgatatca aaatccacat actactttga ggttacgttt taacgtagac
     5701 tcattgttca tgcctaatgg agggactgac agtggaattt tccgtatagt ctgcttcaga
     5761 gtattgcaga tgcccatcaa aaatcgattt ctcactgaca gataaagaaa tggcacaaat
     5821 tagttcgctt gatttgggtt atgttgggga gtcggtaaaa cattttaatc ctgaatttgt
     5881 tcgtggttgt c
//
LOCUS       DAPHBJ010000239         5768 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.241, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000239 DAPHBJ010000000
VERSION     DAPHBJ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.241"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(323..1282)
                     /gene="nrdF"
                     /locus_tag="RHZ76_004129"
     CDS             complement(323..1282)
                     /gene="nrdF"
                     /locus_tag="RHZ76_004129"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDV2814658.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(1292..3436)
                     /gene="nrdE"
                     /locus_tag="RHZ76_004130"
     CDS             complement(1292..3436)
                     /gene="nrdE"
                     /locus_tag="RHZ76_004130"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDV2814659.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYTRTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFTRSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(3409..3819)
                     /gene="nrdI"
                     /locus_tag="RHZ76_004131"
     CDS             complement(3409..3819)
                     /gene="nrdI"
                     /locus_tag="RHZ76_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDV2814660.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(3816..4061)
                     /gene="nrdH"
                     /locus_tag="RHZ76_004132"
     CDS             complement(3816..4061)
                     /gene="nrdH"
                     /locus_tag="RHZ76_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDV2814661.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(4309..4638)
                     /locus_tag="RHZ76_004133"
     CDS             complement(4309..4638)
                     /locus_tag="RHZ76_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDV2814662.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            4790..5134
                     /locus_tag="RHZ76_004134"
     CDS             4790..5134
                     /locus_tag="RHZ76_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HDV2814663.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(5171..5620)
                     /gene="alaE"
                     /locus_tag="RHZ76_004135"
     CDS             complement(5171..5620)
                     /gene="alaE"
                     /locus_tag="RHZ76_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HDV2814664.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         1479 a         1420 c         1429 g         1440 t
ORIGIN      
        1 cgatatatta cccaatggaa atagtcactt ttttctaccc taacatactg agaatctgag
       61 gcaacccctg atggcaaatg tggcatgaat attgatgcga gcaaatcgcc ggaatttccc
      121 gtgatataag ggctgagagc aaatcgagaa agttgatatt ttttgtcaaa gatagcgaaa
      181 aaacgcctga atattgtggt ttcaggcgaa taatgcaaca gagtatcagt gtagatcacc
      241 acaaattatt tgcgtgataa aaagctattt ggcgggaatt atttccctgc tgcgggtagt
      301 gatatttttg aaaataacac cctcagaaat tccagtcttc atcttctgtt tcaaccgctt
      361 tccccatcac ataagaggag cctgaaccgg aaaagaaatc gtgattttca tcggcattcg
      421 gcgaaagcgc ggcgaggatt gccggattca cttccgccat ttctgcggga aataacggtt
      481 cgtagcccag attcatcaaa gccttattgg cgttgtaaca gagaaacgct ttcacatcgt
      541 cagcccacgg ggtttcggcg tacagctcat cggtgtattg caactcgttg tcgtagagtt
      601 ccagcaacaa atcgaaggcg aaactcttca actcttcacg ttgtcccaga gatatctttt
      661 ccatgttttt ctgatattta tagcctatgt agtaaccgtg gactgcttca tcgcggataa
      721 tcagacggat caggtccgcg gtattggtca gctttccgcg gctggaaaaa tacatcggca
      781 gccagaaacc ggaatagaac aaaaaagatt caagaaacac actggcgatt ttctttttca
      841 gcggatcatc accgcgataa tgttgctgaa taatctgagc ttttcgctgc aacggtgcgt
      901 tttcttcact ccaggcgtag gcggcatcga catctttggt ctggcatagc gtcgagaaaa
      961 tcgaactgta agagcgggca tgaaccgctt ccataaagct gatattcgat aataccgctt
     1021 cttcatgagg cgtgagtgca tcgggcatca gagaaggcgc gccgataaca ttttgcagcg
     1081 tgtcgagcag cgtcaggcca gtaaaaacgc gcatcgtcag ttgttgttct acgacagtta
     1141 atgtctgcca ggcaggaata tcgttcgaca gcggcacctt ttctggtagc cagaaattgc
     1201 tggtcaggcg attccacacc tccagatctt tatcgtcaga tatcttgttc cagttgatgg
     1261 cgctgatacg tgagagtttc atagatattc cttaaagtgc acaggagacg cagccttcaa
     1321 tttcagtgcc ttccagcgcc atctgacgca ggcggatgta atagagcgtt ttgataccct
     1381 tgcgccaggc gtaaatctgc gctttgttga tatcgcgagt ggtggcggta tcggggaaaa
     1441 aaagcgtcag cgacagcccc tgatcgacat ggcgagtcgc ttccgcgtag gtgtcgatga
     1501 tcttttctgc gccaatttcg taagcgtcct gatacagcgc cagattctcg ttagtcataa
     1561 acggggcagg gtagtaaacg cgtcctgttt tgccctcttt gcgtatctct actttcgcca
     1621 caatcggatg aatactcgac gtagcatggt tgatataaga gatagaaccg gttggcggca
     1681 ccgcctgaag attctggttg tatatgccgt agcgcatcac gtcgtcgcgc agctgcgccc
     1741 acatctcacg ggtaggtaac gtaataccgc tacgggtaaa cagttcgcca actttcgccg
     1801 ttttcggctg ccagttccct tgcagatatt ggctaaaata ttcaccactg gcatagcgtg
     1861 actgtttgaa cccggcgaag gtttcaccgc gttcgcgtgc cagcaacatc gaggtacgca
     1921 gtgcatgcca ggtgatggca tagaaataga gattggtgaa atccagtgct tccggcgaac
     1981 cataagcgat gccttctcgc gccagatagc cgtgtaaatt catctgcccc agtccgatgg
     2041 cgtgcgaggc ggcatttccg gcttcgatgg acggcacgct gcggatatga ctcatatctg
     2101 atactgccgt taaaccgcgc acggcagtct ctaccgtgcg ggcaaaatcg ggggaatcca
     2161 tggtgtgcgc aatattcaac gaacctaaat tgcaggaaat atcatggcct gtgcgggtat
     2221 agtcgagatt ctcgtcatac tctgaggcgc tgttaacctg caaaatttct gagcagagat
     2281 tactcatatt tatgcgcccg gcgatagggt tagcacggtt taccgtgtct tcatacatga
     2341 tgtagggata gccggactca aactggattt ctgccagtcg ctggaagaaa tcacgggcgt
     2401 tgaggtattt tttgcgaatg cgttcatcgg caaccagttc gtcatagtgt tggctgatgg
     2461 ccacatcggc aaacggcttg ccataaactc gctctacgtc ataaggcgaa aacagcgcca
     2521 tctgcgcatt ctcttttgcc agatggaaag tgatatccgg gatcaccacg ccaagcgaca
     2581 gtgttttaat gcggattttt tcgtcggcat tttcccgttt cgtgtcgaga aaacgcagaa
     2641 tatcgggatg atgagcatgc aaatagacag caccagcccc ctgacgagcg ccgagttggt
     2701 tggcatagga aaatgcgtct tccagcattt tcatcaccgg aattacgcca gaagattgat
     2761 tttcaatacg tttaattggc gcgcccgctt ctcgcagatt cgacagcaaa aatgctacgc
     2821 cgccgccgcg tttcgacagt tgcagtgcgg aatttaccgc ccgaccaatc gactccatat
     2881 tgtcttcaat acgcagcaaa aaacaggaaa ccagttcgcc gcgctgctgc ttaccgcagt
     2941 tgaggaatgt tggcgtggct ggctgaaagc gtcctgacag catttcatct gtcagttgca
     3001 acgccagcgt ctcatcgcct tgtgccagcg tcagcgccac catcgtgact cgatcggcaa
     3061 aatcttccag ataacgttta ccgtcgaatg tcttcaacgt atagctggtg taaaacttcc
     3121 atgccccgag gaatgtctgg aaacgaaaac cgctggtgtg tgcgtgggca aacagcgtaa
     3181 tgacaaagtc gcgagagtag cgattaagaa cgctttcatc atagtaacct tcgttgacca
     3241 gccagttcag gcgctgctgc tggctactga aggtcacact gttcggacgc acatgcgtcg
     3301 caataaaggc gtcaacggcc tggcggtctt tatcgaactg aatgcgacct gcgctatcgt
     3361 agaggttaag catcgcattc agcgcgtggt aatccatcgt ttcctgcgtc aggcattctg
     3421 cggttgtcgt tgccaaaatt cggttactcc tttacgaacg ttttcgatat cgctttgcgt
     3481 acccatgagt tcaaaacggt acagccacgg cacgccgcat ttccgggcaa tcacatctcc
     3541 ggcgcggcca tacgcctcac caaagttgcg attaccagaa gcaataacgc cgcgaagcaa
     3601 cgcccggttg tgctcgtcgt ttaaaaagcg aattacctgt cgtggcaccg cgccagccgt
     3661 accgccgccg ccgtaagagg gcacgatcag gatgtaaggc tcgtctacct gaatccgttc
     3721 ccgctcattg agcgggatgc gcaccgcggg cagacctaaa cgttcgataa aacgctgcgt
     3781 gttttcggag ctgctggaga agtagacgag ctggctcatg cactggccgc gtgtggcgct
     3841 ggatgcagac ggttaatcat gtccggacgg aaaccagacc agctaagatc gccagcaatc
     3901 actaccggca actgacgaaa gccctgagca cgcaacgctt ctgccgcttc aggaacgcga
     3961 tcgacattaa tcatttcaaa atcaaagccc cggttttcca tcgcccgttt ggtggcgtgg
     4021 cactgaacgc aatcgttacg agtgtaaata gtaatgcgca tgattcgtat ttccgtttaa
     4081 aatgaagata cggcgcgatg atacgcgtcg ggttgtctct ctgttgatac agagatacta
     4141 gatgtagttg aaaaaagatt caaccacaca atatatagca aatagtggtc gaaattaccc
     4201 tggatatgag cgtgacgggg tagggggatt tttgtgattc accaggcaaa aagaaacccc
     4261 gaagacaggc ttcggggtca aagacgcgta tttattatca tttttgcact acgatttgcg
     4321 catgcttaac agtgcgccga taaaaatacc taccgcagct gctgtaccca cgctacacca
     4381 gggtctttca cgaacaaaag aatccgcgca gccaacggca tcgcgcgcgg cttgctggac
     4441 gcgagtacga ccatgcattc gcgccctggt ttctttcagc aatgcctgag ctttactgcg
     4501 tgcggcttca gcttcccctt tggcgtcgct gccccaggac ttcaatacag attcgaggct
     4561 gtcagctaat tgattgacat cattctggat atcctgcacg ccatcatcaa cgtcgttgcg
     4621 gttcggtcgg ttaaacatat aatcccccat tagaattcga tgtaattttc agtgtagacc
     4681 ataattttct taactgaggt tcatcgcggc tttatggact tttctgaaag cgttgtgaat
     4741 gcgcaatcag gtaaggtagg cccgcggttg ataataacga ggatcaaata tgtatttacg
     4801 accagacgag gtggcgcgcg tacttgaaaa agtcggtttt actgtcgatg tggtaaccca
     4861 gaaagcgtat ggttaccgcc gtggggaaaa ttatgtctat gttaatcgcg aagcgcgaat
     4921 gggacgtacc gctctggtaa ttcatccaac attaaaagaa cgcagttcga cactggctga
     4981 acccgcttcc gatattaaaa cttgcgatca ttaccagcaa tttccgctct atttagcagg
     5041 cgagcgacac gagcattacg gtatcccgca tggctttagt tcgcgtgttg cgcttgaacg
     5101 ttatttgaat ggtttatttg gcgaagccag ttaaagacgc gactggcgat gccagtcgcg
     5161 aaaagaagag tcaggctttt acctgctggt aacggctgac tttaaacagt cggcggcaat
     5221 aatcgaggaa gtagccataa accgccccca tcaacatcga aacaacgatg tttgaactga
     5281 ccgccgccat aatctgatgc cagtctgcgc ccaccactaa caagatcgcc acatacaccg
     5341 gtgactggaa cgtcacataa gccaggatat ccgccagatt ttttatccag cccgacgggc
     5401 taactttgcg tgccgcgcgc ataaacagat cacggtacat accgtatggc catgcaatta
     5461 agatgttcac cggaatcgct accaatctgg aataaaaaga ctgttcgaag ctcattccgg
     5521 agaggaaaac ttcaatacac atgttcacga cagaacagta aacaaccatc gcgaacgtat
     5581 ctgcaactgc atgacgcaag cgtgactgcg gtgagaacat gctaatgctc cttaataaaa
     5641 gcgtaatggg atgttaatgg agatgaaaac agtggatctc attgccatct gcattttcgc
     5701 atagcgtata tcgctggcta tgactaatca attagtgaaa aatttttatt tagtcagttt
     5761 ttgtagat
//
LOCUS       DAPHBJ010000240         5635 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.242, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000240 DAPHBJ010000000
VERSION     DAPHBJ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.242"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..167)
                     /locus_tag="RHZ76_004136"
     CDS             complement(<1..167)
                     /locus_tag="RHZ76_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical efflux pump membrane protein ArsB"
                     /protein_id="HDV2814665.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSAMLGAGLALISGVVHVG
                     DIPVVWNIVWNA"
     gene            complement(215..1966)
                     /gene="arsA"
                     /locus_tag="RHZ76_004137"
     CDS             complement(215..1966)
                     /gene="arsA"
                     /locus_tag="RHZ76_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter ATPase subunit ArsA"
                     /protein_id="HDV2814666.1"
                     /translation="MKFLQNIPPYLFFTGKGGVGKTSISCATAIRLAEQGKRVLLVST
                     DPASNVGQVFDQAIGNTIRPVTAVPALSALEIDPQEAARQYRARIVDPIKGLLPDDVV
                     NSISEQLSGACTTEIAAFDEFTGLLTDASLLTRFDHIIFDTAPTGHTIRLLQLPGAWS
                     SFIESNPDGASCLGPMAGLEKQREQYAHAVEALSDPERTRLVLVARLQNSTLQEVART
                     HEELAEIGLKNQYLVINGVLPEAEAEHDALAAAIWQREQEALANLPAGLSELPTDTLL
                     LQPVNMVGVSALKGLLDTRSEALPLPVTNILYTPENLSLSGLVDDIARSEHGLIMLMG
                     KGGVGKTTMAAAIAVRLADMGFDVHLTTSDPAAHLSTTLNGSLKNLQVSRINPHDETE
                     RYRQHVLETKGRDLDEAGKRLLEEDLRSPCTEEIAVFQAFSRVIREASKRFVVMDTAP
                     TGHTLLLLDATGAYHREIAKKMGSKGHFTTPMMQLQDPDRTKVLLVTLPETTPVLEAA
                     NLQADLERAGIHPWGWIINNSLSIADTRSPLLCQRAHQELPQIEAVKDQHADRIALVP
                     VLASEPAGIEKLRELMS"
     gene            complement(1984..2346)
                     /gene="arsD"
                     /locus_tag="RHZ76_004138"
     CDS             complement(1984..2346)
                     /gene="arsD"
                     /locus_tag="RHZ76_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter metallochaperone
                     ArsD"
                     /protein_id="HDV2814667.1"
                     /translation="MKTLTVFDPAMCCSTGVCGSDVDQVLVDFSADVQWLKERGVQVE
                     RYNLAQQPMSFVHNEKAKSFLDASGAEGLPLLLLDGETVMAGRYPKRAELARWFGIPL
                     EKVGLAPTSCCGGNTSCC"
     gene            complement(2398..2748)
                     /gene="arsR"
                     /locus_tag="RHZ76_004139"
     CDS             complement(2398..2748)
                     /gene="arsR"
                     /locus_tag="RHZ76_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HDV2814668.1"
                     /translation="MTLTALKLFKNLSDETRLGIVLLLREMGELCVCDFCTALDESQP
                     KISRHLAMLRESGLLLDRKQGKWVHYRLSPHIPSWAAQVIEQAWLSQQDDVQAIARKL
                     ASANCSGSGKAVCI"
     gene            complement(3104..4078)
                     /locus_tag="RHZ76_004140"
     CDS             complement(3104..4078)
                     /locus_tag="RHZ76_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HDV2814669.1"
                     /translation="MQAPGIPENEEQRLKSLYITGLLDTRDDERFERLTRLARKAFQV
                     PVALISLLDRERQWLLACQGVGVRETPRDISFCGHAILQEGPFIIHDAAADARFHDNP
                     LVTGEPHIRFYAGQPVSLPDGTVAGTLCLIHTVPRAFTDEDIASLRDLACIVEDEFEA
                     ISMAMTDSLTGIPNRRGFYRAGEKLFRAMNRQDASFSLIYFDLDRFKPVNDLWGHAEG
                     DEVLKIFAGFLHQHLEPGEIAGRLGGDEFAALIRRNGNTQSYLHALSASLDHYNDTSG
                     KPYNINYSFGELINNADHYTTLAEMIGKCDEVMYLKKKRKTLSRKTEP"
     gene            4358..5326
                     /locus_tag="RHZ76_004141"
     CDS             4358..5326
                     /locus_tag="RHZ76_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HDV2814670.1"
                     /translation="MKDQITHLPDNADRSVAKQKFKITNWPTYNKALINRGSITFWLD
                     DEAIQAWYESAAPSSRGRPQRYSNLAITTVLVIKRVFRLTLRAAQGFIDSIFSLMNVP
                     LRCPDYSCVSRRAKSVNVSFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRI
                     WRKLHLAVDSKTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRSAAADGAYDTRLCHD
                     ELRRKKISALIPPRKGAGYCPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETA
                     MYRVKQLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIA"
BASE COUNT         1418 a         1467 c         1449 g         1301 t
ORIGIN      
        1 gcgttccaga cgatattcca caccaccgga atatcgccca catgcacaac gccagatatc
       61 agagccagtc ccgcgcccag cattgcactc cagccgatcc ctaatccctt tggttgccag
      121 ataaccaaaa caatggtcag gacaaaaata gcgccggcca gtaacataaa acctcctgac
      181 agggcgactt tgtcgccctg tatatgtcaa aaaattagct catcaactct ctgagctttt
      241 caataccggc gggctctgac gccagcaccg gaacaagcgc tatacggtca gcatgctgat
      301 ccttaacagc ctcaatctga ggcaattcct gatgagcacg ctgacaaagc aacggagaac
      361 gcgtatccgc aatggaaagg ctgttattga taatccagcc ccacggatga atccccgctc
      421 tttcaaggtc agcctgcagg tttgccgctt ccaggaccgg tgtggtttca ggaagcgtaa
      481 ccagcagaac tttggttcta tccgggtcct gaagctgcat catgggggta gtaaaatgac
      541 ctttactccc catttttttg gcaatctctc ggtggtaagc cccggtcgca tccagcaaca
      601 gcagcgtgtg tccggtaggg gcggtatcca tgacaacaaa gcgcttactc gcttcacgaa
      661 ttacacggga gaaggcctga aacacggcaa tttcttctgt acagggagag cgtaaatcct
      721 cttccagtaa ccgtttccct gcttcgtcca gatctcttcc cttcgtctca agaacatgct
      781 ggcgatagcg ttcggtttca tcgtgagggt tgatgcggct gacctgcagg tttttgaggc
      841 tgccgttcag cgttgtactc aggtgcgcag caggatcaga ggtcgtgaga tgcacgtcaa
      901 atcccatgtc tgccagcctg acggcgatgg cagcagccat cgtggttttc cctacaccac
      961 ctttgcccat cagcataatc aggccgtgtt cactgcgagc gatatcatcg accaggccag
     1021 agagagataa gttttcaggc gtgtacagga tattcgttac cgggagcggt aatgcctcag
     1081 aacgggtatc cagcagtccc ttcaatgctg aaacaccaac catattgact ggctggagta
     1141 acagggtatc tgtcggtagc tcagataaac cggcaggaag atttgccagt gcctcctgct
     1201 cacgttgcca tatcgccgcg gccagggcgt catgttcagc ttcggcttca ggcagcacac
     1261 cattaatcac cagatattga tttttcaggc caatctcagc cagttcctca tgagtgcggg
     1321 cgacttcctg cagtgtagaa ttttgcagtc gtgcaaccag aaccaggcgg gtacgttcag
     1381 gatcggacaa agcctctacc gcatgagcat actgctcacg ctgcttttcc agcccggcca
     1441 ttgggccaag acaggaagca ccatctggat tactttcaat gaagctactc caggcaccgg
     1501 gaagctggag cagacgaatc gtgtggcccg tcggcgctgt atcaaaaatg atgtgatcaa
     1561 agcgggtcag cagggaagcg tctgttagta agcccgtgaa ttcatcgaac gcagcaatct
     1621 cagtcgtaca ggctcctgaa agctgctcgc tgatactgtt aacaacgtca tcaggcaaaa
     1681 gacctttgat aggatcaacg attctggccc gatattgccg ggcggcttcc tgcgggtcaa
     1741 tctccagtgc ggaaagtgca ggaactgctg tcacagggcg aatcgtgtta ccgatagcct
     1801 gatcgaatac ctgaccaaca ttggaggccg ggtcggtact gaccagcaaa acccgcttac
     1861 cctgttcggc aaggcggata gccgtcgcgc aggaaatgga agtttttcct acacctccct
     1921 tgccagtaaa aaacaggtaa ggcgggatat tctgtaagaa tttcatatgt cctcctgaca
     1981 tattcagcaa caggaagtat taccaccaca gcaactggtg ggagctaacc ctaccttctc
     2041 cagcggaata ccgaaccagc gagccagctc agcgcgtttt gggtatcgcc cagccatcac
     2101 cgtttcgccg tccagcaaca gcagcggaag cccttctgcg ccggatgcgt cgaggaagga
     2161 tttcgctttc tcattgtgaa caaagctcat gggctgctgc gccaggttgt aacgttcaac
     2221 ctgtacacca cgttctttca gccactgcac atcagcagaa aaatcaacca gaacctgatc
     2281 gacatctgaa ccacatacac cggtactgca gcacattgcc gggtcaaaca ccgttaacgt
     2341 cttcattctg aatacctcat acattcgaaa aaacacatat gttcaggcaa atttttttta
     2401 gatgcaaaca gccttaccgc taccagagca gtttgccgat gccagcttac gggcgatggc
     2461 ctgtacgtcg tcctgttggc ttaaccaggc ctgctcaatc acctgagcag cccaggaagg
     2521 aatatgcggg gataagcgat aatgaaccca tttcccctgc ttgcgatcca gcaatagacc
     2581 gctttcccgg agcattgcca gatgacggga aatcttgggc tgggactcat ccaacgccgt
     2641 gcagaaatcg cagacgcaca gttctcccat ctccctgagc agcaggacaa tacccagacg
     2701 ggtttcatcg gacaggtttt tgaaaagctt aagggctgta agggtcatta ttcctcctgt
     2761 tcggttgcgt aaagcatacg cccggagaca aaaaaaacaa ccatacattc ggaaaatcga
     2821 atgtgtgaaa gtgtcagatg acgctgtcac gctggtatac gtccgttact ttccggggaa
     2881 aatacatgtg tgttacagag atccgacttt accttttttc ggatctcaga agtggagaag
     2941 aggaattatt gcagcatgga cacaaaaaac gagtaattgt tgcgatgagt ggactattgc
     3001 accatgagca aatataaatg actttatgcg tcctgcggtt aaatatattg ttcatgccgt
     3061 ccggctgaac cccgggtcag gggatcagcc gggaaggaga acgtcacggt tctgttttcc
     3121 gcgacagggt ctttcttttt ttcttcaggt acatcacttc gtcacattta ccgatcatct
     3181 ccgccagtgt cgtataatgg tctgcgttat tgatcagttc gccaaaggaa taattgatgt
     3241 tgtagggctt gcccgaggtg tcattgtaat gatcgagact ggcactcagc gcgtgcagat
     3301 aagactgagt gttaccgttt cgccggatca gggcggcaaa ctcatccccg ccgaggcggc
     3361 ctgctatttc acccggttca aggtgctgat gtaaaaagcc ggcaaagatc ttcagcacct
     3421 cgtcaccttc cgcgtgtccc cagagatcgt taaccggttt gaatctgtcc agatcgaaat
     3481 agatgaggct gaacgacgca tcctgccggt tcattgcgcg gaagagtttt tcgccggcgc
     3541 ggtaaaatcc gcgccggttg ggaatgcccg tcagactgtc cgtcatggcc atgctgatgg
     3601 cctcgaattc gtcctccacg atacaggcca ggtcccggag ggacgcaatg tcctcatccg
     3661 tgaaggcgcg gggaacggtg tggatcaggc acagcgtgcc ggccaccgtg ccgtccggca
     3721 ggctcaccgg ctggccggcg tagaaccgga tgtgtggctc accggtaacc aggggattat
     3781 cgtgaaatcg cgcatcggca gccgcatcgt gaatgataaa aggcccttcc tgcagaatgg
     3841 cgtgcccgca gaacgaaata tcacgtggag tttcgcgcac gccgacgccc tggcaggcga
     3901 ggagccactg gcgctcgcga tccagcaggc ttatcagggc caccgggacc tgaaaggctt
     3961 tgcgggccag ccgcgtcagg cgttcaaaac gctcatcgtc acgggtatca agaaggccgg
     4021 taatatacag ggacttcagc ctctgttctt cattctccgg tataccgggt gcctgcatgt
     4081 aatctcctgg tatgtacaaa gtatatatgc agatgaaaag gtgtttggat ctgactgagt
     4141 gtaattatag ttcacgactt atctgccagc ggctgaactt caggtacagg aattcacgtt
     4201 tatcactcgt accagactct ccggaggacc aggggaatga ccgggtctgt tacggtgagg
     4261 gaatctgtaa acctcaggat ttttgccggg aaagtacgta taactgctat cttgattagt
     4321 aaatgggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga tcacgcatct
     4381 tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca actggcccac
     4441 ctacaataaa gccctcatca accgtggctc cataactttc tggctggatg atgaagctat
     4501 tcaggcctgg tatgagtcgg cagcgccttc ttcacgaggc agacctcagc gctattctaa
     4561 ccttgccatc acgactgtgc tggtcattaa acgcgtattc aggctgaccc tgcgcgctgc
     4621 gcagggcttt attgattcca ttttttctct gatgaacgtt ccgctacgct gcccggatta
     4681 cagctgtgtc agcaggcggg caaagtcggt taatgtcagt ttcaaaacgc ccacccgggg
     4741 tgaaatcgca cacctggtaa ttgattccac cgggctgaag gtcttcggtg aaggcgagtg
     4801 gaaagtcaaa aagcatggcc aggaacgccg ccgtatctgg cgtaagctgc atctcgccgt
     4861 tgacagtaaa acacatgaaa tcatctgcgc tgacctgtcg ctgaacaacg ttacggactc
     4921 agaggccttc cccgggttaa tccggcaaac ccaccggaaa atcaggtcag ccgccgccga
     4981 tggcgcttac gatacccggc tatgtcacga tgaactgcgg cgtaagaaaa tcagcgcgct
     5041 tatccctccc cgaaaaggtg cgggttactg tcccggtgaa tatgcagacc gtaaccgtgc
     5101 agtggctaat cagcgaatga ccgggagtaa tgcgcggtgg aaatggacaa cagattacaa
     5161 ccgtcgctcg atagcggaaa cggcgatgta ccgggtaaaa cagctgttcg ggggttcact
     5221 gacgctgcgt gactacgatg gtcaggttgc ggaggctatg gccctggtac gagcgctgaa
     5281 caaaatgacg aaagcaggta tgcctgaaag cgtgcgtatt gcctgaaaac acaacccgct
     5341 acgggggaga cttacccgaa atctgattta ttcaacaaag ccattcactg gatgtggaag
     5401 agaatatcat atcactatgt tgcaactgcc ataaacaaat tcatcttggc aaggggtttg
     5461 aagatatgtt gaggaaaata tatgccgagc ggaaagatgt attaaaaaaa gcgggaatcg
     5521 agatattatt agaggatttg attctttttt acaagatgga aggtaattaa tcttcgagaa
     5581 gatgcatgct tatttttatt ttttagcaat gaatgtgaaa tactgcctga ctatc
//
LOCUS       DAPHBJ010000241         5580 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.243, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000241 DAPHBJ010000000
VERSION     DAPHBJ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.243"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..176
                     /locus_tag="RHZ76_004142"
     CDS             <1..176
                     /locus_tag="RHZ76_004142"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="catalyzes the hydrolysis of S-D-lactoylglutathione
                     to D-lactic acid and reduced glutathione; plays an
                     important role in cellular detoxification using
                     glutathione; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDV2814671.1"
                     /translation="LRAKNQITLPVILKNERQINVFLRTEYIDLINVINEETLLQQPE
                     ERFAWLRSKKDRF"
     gene            248..1606
                     /gene="mltD"
                     /locus_tag="RHZ76_004143"
     CDS             248..1606
                     /gene="mltD"
                     /locus_tag="RHZ76_004143"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HDV2814672.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1654..2250)
                     /locus_tag="RHZ76_004144"
                     /pseudo
     CDS             complement(1654..2250)
                     /locus_tag="RHZ76_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(2281..3081)
                     /locus_tag="RHZ76_004145"
     CDS             complement(2281..3081)
                     /locus_tag="RHZ76_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDV2814673.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3322..4236
                     /locus_tag="RHZ76_004146"
     CDS             3322..4236
                     /locus_tag="RHZ76_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2814674.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4233..5036)
                     /gene="dkgB"
                     /locus_tag="RHZ76_004147"
     CDS             complement(4233..5036)
                     /gene="dkgB"
                     /locus_tag="RHZ76_004147"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HDV2814675.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKEGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLPPEWD"
     gene            complement(5199..5275)
                     /locus_tag="RHZ76_004148"
     tRNA            complement(5199..5275)
                     /locus_tag="RHZ76_004148"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5239..5241),aa:Asp,seq:gtc)
     gene            complement(5330..5445)
                     /gene="rrf"
                     /locus_tag="RHZ76_004149"
     rRNA            complement(5330..5445)
                     /gene="rrf"
                     /locus_tag="RHZ76_004149"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1467 a         1350 c         1376 g         1387 t
ORIGIN      
        1 agttacgggc aaaaaatcaa ataacactac ccgtaattct gaaaaatgag cggcaaatta
       61 atgttttttt aagaacggaa tatattgatt taattaatgt aattaatgaa gaaacattat
      121 tgcaacaacc tgaagagcgt tttgcatggt taaggtcaaa gaaagatagg ttctgataaa
      181 actttcttgt catcggctcc gttcgccgtt atgatcggtc gtcttttaag caactattga
      241 cacacacatg aaggcaaaag cgatattact cgcctctgtc ctgctcgtgg gttgccagag
      301 taccggcaac gttcaacagc acgcacagag cctttctgca gctggtcaag gggaagcagc
      361 aaagtttaca agtcaggcac gatggatgga cgatgggacg tctatcgcgc cagatggtga
      421 cttgtgggct ttcattggcg acgagctaaa gatgggaatt ccggaaaatg accggattcg
      481 cgaacagaaa cagaaatatt tacgcaataa gagctatctc cacgatgtaa ctttacgggc
      541 agagccgtat atgtactgga tagcagggca agttaaaaaa cgtaacatgc ctatggaact
      601 ggtactacta cccatagtgg agagcgcttt tgatcctcac gcaacgtctg gcgccaatgc
      661 cgcaggcatc tggcagatca ttccgagcac ggggcgcaat tatggtttga aacagacccg
      721 caattatgac gcgcgtcgcg atgttgttgc ttcaacaact gccgcgctga atatgatgca
      781 gcgtctgaac aaaatgtttg acggcgactg gcttctgacc gtagcggctt ataacagcgg
      841 cgaaggtcgg gtcatgaagg caattaaaac gaacaaagcg cgtgggaaat ccacggactt
      901 ctggtcgtta ccgttgccgc aggaaacgaa gcagtacgtg cctaaaatgc tggcattgag
      961 tgatattctc aaaaacagca agcgttatgg cgtacgtctg ccaacgaccg atgaaagccg
     1021 tgctctggcg cgtgtgcacc tgagtagccc ggttgaaatg gcgaaggttg cagatatggc
     1081 ggggatttcc gtcagcaagc tgaagacatt caacgctggc gtgaaaggct ccacgctggg
     1141 cgcaagtggg ccgcagtacg tgatggtgcc aaagaagcat gcagatcaac tgcgtgaatc
     1201 tctcgcttca ggcgaaattg ctgctgtaca gtcgacgctg gttgccgaca atacgccgct
     1261 taacagccgt gtttacaccg tacgctctgg cgacacgctt tcaagcatcg cttcacgtct
     1321 cggcgtaagc accaaagatt tgcagcagtg gaacaaactg cgcggatcta agctgaagcc
     1381 aggccaaagt ttgacgattg gtgcaggcag tagcgcacag cggttggcaa acaacagcga
     1441 tagcattacg tatcgtgtgc gcaaaggcga ttcgctttca agcattgcta aacgccacgg
     1501 cgtgaacatc aaagatgtga tgcgctggaa cagcgacact gcgaatctgc aaccaggcga
     1561 caagctgacg ttgtttgtga aaaacaacaa catgccagat tcctgacaaa ccagataata
     1621 aaaaggcacc gattcccccg gtgccttttt tatttatgcc gctttatgtg cttctaccat
     1681 gatgatatca ctggtgaaag agccatcagt ctgcaaggaa aaatacgttc tcacctctgt
     1741 cgatgcgctc tgttggtaaa tgcgaatagc gtctactaac gcttctggcg tacgcattct
     1801 cacgacccat gaagaaaatt ccagcggtaa cttatctgta attaaattat caactatcag
     1861 attggcttca ttgattaacg tcaaccactc accgctggcg tagtttcgta cgtgagaggt
     1921 atcgcgtaat gcttctaccg tctgtaacca gatgtcgcgc actgggtggc ccggagacat
     1981 tacgtccatc acaatcagcc taccgccagg tttcaatatc cggttcactt cacgcagtgc
     2041 tgcaccaaca tcatgccagt gatgggcaga ataacggctg ataacaatat caaatgcgtt
     2101 atcggcaaat ggcagacttt cggcataacc ctggcgggtg gtgatatttt tcagttgccg
     2161 gacttcggca gcttgtgcca cgacatccag catgtgggca gataagtcat acgccaccac
     2221 cgtgctcacg ttttgcgcag cgacaaacgt tggtctgccc tgtggcagac ctgacatacc
     2281 ttatttatca ggcttgccgg gactgaattc aacgagtagc ggattgtgat cggaagcgcg
     2341 cgtaaccagt acagaagctt cactgacgtt cagaccacgg tagaaaacaa aatcgagcgg
     2401 gcgaccaaac gcccgacggc gctgatcatc ggtaaaacgc acctggcgca gcgacatttc
     2461 ccgcgcaaag cgatataacg cgttcatcct tctacggctc caggcattga aatctcccgc
     2521 cataatgacc gggccgctgt ggtgagctat ctgatcacca ataggaagta actgcttact
     2581 atagacatcc acgcccagac tgaagttgac ggcgtgtata ttaaccacca tcaacaggcg
     2641 ggtgtcaggt aatggataga ccgtcaccag tgccgacttc gccagacgca aaatgggttc
     2701 tcgttcacgt aacgggcagc aatacactgg atgtgccgcc gaaagggtca ttacgccaga
     2761 aggatgttgt ggcagcacga aagcgggtac ctgatcggcg gcaagatagt tagcggtcgc
     2821 aaactgtact aactctggcg ttgtctgcgc ttcctgcaat aacaccagat gtgcatcttt
     2881 gccgtagttc tttaataccg acaaccattc agcgcgttgc tgtttgtata tgttccacac
     2941 caggatccga atacgctctt cggtacttaa cggttcccca ggtggtaatg cctggccgat
     3001 gctcgcaaaa gaccccggcg gtaagatcct ttccgcaggt tgtccggcaa catagcgcat
     3061 ggcataggtg ttttttcgca cttttgactt tgaaacctct attacgtcaa cccttccgca
     3121 ctcgggaggg ttctactgtt atagggattt tagctcactc tttcaacgag caattgtata
     3181 ttgttatgta agcaagtgct tacaccggtc agaattttgc tcattatgga gaacattaaa
     3241 tcgcatgaca tatcacgcat cctgatggtt taatgacaaa taaattcaaa atatctcttc
     3301 tattttgcaa caggagcaaa aatgaaagcc acgtcggaag aactcgccat ttttgtttcg
     3361 gtcgtcgaaa gcggcagctt tagccgggca gcggaacaat tagggcaagc aaactcagcg
     3421 gtaagccggg cggtgaaaaa gctggagatg aaacttggcg ttagcctgct taatcggacc
     3481 acgcgacaac ttagcctgac ggaagaaggc gagcgttatt ttcgtcgcgt acagtcaatt
     3541 ttgcaggaga tggcagcggc agaatcagaa attatggaga cgcgtaatac accgcgtgga
     3601 ctgttacgga tcgatgccgc aactccagtg gtgctgcact ttctgatgcc gttaattaag
     3661 cctttccgtg aacgctatcc ggaagtcact ttgtcgctag tctcctccga aacgattatt
     3721 aatttgatcg aaagaaaagt ggatgtcgcg atacgcgctg gtacgttaac ggattccagc
     3781 ttacgtgcca ggccgttatt taacagttat cgaaaaatta tcgcctcccc cgattatatt
     3841 tcccgctacg ggaagccaga aacgatcgac gatttaaagc aacatgtttg cctgggattc
     3901 actgaacccg cttccctcaa tacctggccg atagcctgta gtgatggaca attacatgag
     3961 gtgaagtacg gtttgtcatc caatagtggg gaaacactga aacagctttg cctgagtggc
     4021 aacgggattg cgtgtttgtc cgactacatg atcgacaaag aaatcgctcg cggagaattg
     4081 gtggagttaa tggcagataa agtgttgcca gtggaaatgc cattcagtgc cgtctattac
     4141 agcgaccgtg cggtaagtac gcgcatccgg gcttttatcg atttccttag cgagcatgta
     4201 aaaacagctc ccggaggagc tgtcagagag gcttaatccc attcaggagg cagaccttcc
     4261 gggctaacca ggcggtcgtt gcaatccagt gcggcgatcg cttttttatc ttcggcatca
     4321 agctgtaaat tttgtgcctt aagattactt tccaggtttt tacgtttagt agaagaagga
     4381 attactgagt aaccttcccc catagcccac gccagaatca cttgtgccgg agtcgcattg
     4441 tgtttagctg cgatacgagc aataacctca tctttcaggg ccttaccata cgccagcgtc
     4501 atataggaag taatatggat gccgtgctgt ttagcccagg caaccacttt acggttttgc
     4561 agataaggag agagttcaat ctggttagta gcgatgtttt cagcgccaac agcagcaatc
     4621 gccttttcca tcaatgggat cgtgaagttg gaaataccga tctcacgcgt caacccttct
     4681 tttttggctt ccagcagcgc ctgcataaac tcttcaacag agacttcatc gtttggtgac
     4741 ggccagtgga ttagagtcag atcaacataa tcagtacgca atttttgcag gctctctttc
     4801 agactcggga tcaatttgtc tttgctgaga ttttcaatcc agattttagt ggtgatgtag
     4861 agttcatgac gtggcacgcc actttctgca atcgcctgac ctactgcggc ttcgttatca
     4921 tagatttgtg cagtatcaat tgcgcgataa ccaagttcaa gcgccgtttt cacagatgaa
     4981 ataacaacgt cgtctttcag acggaaagta cctaaaccaa atgcagggat agccataata
     5041 tgcctctttt aatgctattt tcgttaactg aggtgattat gggcttcaga ctgttgagga
     5101 aaaagagcgt aaaatgcaga ggatttttgc gattctggca ataatagata tacagacgaa
     5161 aaaaaacctc gagcgttaac tcgaggcttc ttaataagtg gcggaacgga cgggactcga
     5221 acccgcgacc ccctgcgtga caggcaggta ttctaaccga ctgaactacc gctccaccga
     5281 attcttttac aaccaccggt tttatgaccg gcttactgct taatttgatg cctggcagtt
     5341 ccctactctc gcatggggag accccacact accatcggcg ctacggcgtt tcacttctga
     5401 gttcggcatg gggtcaggtg ggaccaccgc gctacggccg ccaagcaaat tcctgtgctc
     5461 tgtcctgtgt cttttgtgct gatactgcgt cggctgcatt cgcgaaagtc agtcacatac
     5521 ttcagtatgc tccttccttc gcttcatttg ccgccttgtc tcagcgcaaa atccttcgga
//
LOCUS       DAPHBJ010000242         5569 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.244, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000242 DAPHBJ010000000
VERSION     DAPHBJ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.244"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..307)
                     /locus_tag="RHZ76_004150"
     CDS             complement(<1..307)
                     /locus_tag="RHZ76_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDV2814676.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     "
     gene            506..1204
                     /locus_tag="RHZ76_004151"
     CDS             506..1204
                     /locus_tag="RHZ76_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV2814677.1"
                     /translation="MIFQKIARLLKSEINGYSWHVGDLLPSETELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFTEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVPGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTYETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(1249..2148)
                     /gene="eamA"
                     /locus_tag="RHZ76_004152"
     CDS             complement(1249..2148)
                     /gene="eamA"
                     /locus_tag="RHZ76_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HDV2814678.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            2343..3530
                     /gene="ydeE"
                     /locus_tag="RHZ76_004153"
     CDS             2343..3530
                     /gene="ydeE"
                     /locus_tag="RHZ76_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HDV2814679.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            3657..3752
                     /gene="mgtS"
                     /locus_tag="RHZ76_004154"
     CDS             3657..3752
                     /gene="mgtS"
                     /locus_tag="RHZ76_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HDV2814680.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(3971..4861)
                     /gene="dgcZ"
                     /locus_tag="RHZ76_004155"
     CDS             complement(3971..4861)
                     /gene="dgcZ"
                     /locus_tag="RHZ76_004155"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HDV2814681.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(5116..5508)
                     /gene="ydeI"
                     /locus_tag="RHZ76_004156"
     CDS             complement(5116..5508)
                     /gene="ydeI"
                     /locus_tag="RHZ76_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HDV2814682.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         1524 a         1341 c         1166 g         1538 t
ORIGIN      
        1 gttgcagtag tttcatcgca aattcaagaa ctgcctgtcc gttcatggtg ccgtagtctt
       61 tcatctcgat aacggctaca ggcattttcc cctgtgcaac ggcttcaatc tccggtttct
      121 gaaagcgaac ttgcgggcca agtaaaacaa cgtcaacttc gttattttta atttttcctt
      181 tagcctctgc aatcgccaat gcggaaatat taacttcaag tgaaatagcg ttagcatgat
      241 caatcatacg tttaaccagc atactggttg acatgcccgc agcgcaaact aacaatatct
      301 ttttcatatt ttcccttact ggtatataac agactacatt gtgtaaatat catatcccgc
      361 agtagctata catcctggta cattgatcac agtaatacag taataaagtt gttcaacatc
      421 agggtaataa ttaaacgact cggccatacg ataaaccaat caggactcaa ctccttgttt
      481 tcaatggctc ttaaaaggtg caaaagtgat ttttcaaaag attgcccgct tacttaaatc
      541 tgagatcaat ggctattcat ggcacgttgg tgatttgttg ccgtcagaaa ctgaactcgc
      601 agttcgttat aatgtttcgc gtaataccct gcgtaaggca ttatccttgc tggaaggcga
      661 aggaattatt cacagaaagc atggttcagg aacatacatt caaaaaaaga attttgttgc
      721 acacattgat cacatgaaca gcttcactga aattgcgcat aaaagcggca aagaggcagg
      781 gagtcagatt atgaaatttg aagtgcagga tgcttcaccc actattgcaa ctgagctgaa
      841 tttagtgccc ggtgagcagg tttattacat aaagagactg cgattcattg aggataatgc
      901 ggcacaatta gaagaaacgt ggatgtcagt ggcacgtttt cctgatttaa ccgtatcaca
      961 tatgcaaaaa tccaaatttt cgtatattga gaacgaatgc gggatcaaaa tcattggcac
     1021 ctatgaaact ttctccccta cttttcctac cccagaaatc gccagtattt tacggatcag
     1081 cccacgggat cccatactta aaattcagac ccaggctgtg gacagtaact ctattccgct
     1141 ggattattcg ttactttaca gcaatatttt cgagttccag gtaaagtact ttttcccgcg
     1201 ataaaagcag acgcacagag tgctgacatc acattctgtg cgtatggttt agcctctaac
     1261 ctttaccgct ttacgccacc gcaagccaaa tacattgata tacagcccgg tcataatgag
     1321 caccgcacct aaaaattgca gacccgttaa gcgttcatcc aacaatagtg ccgcacttgc
     1381 cagtccgact acgggcacca gtaacgataa cggtgcaacc cgccaggttt catagcgtcc
     1441 cagtaacgtc ccccagatcc cataaccaac aattgtcgcc acaaacgcca gatacatcag
     1501 cgacaagatg gtggtcatat caatagtaac cagactgtga atcatggatg cggaaccatc
     1561 gagaatcagc gaggcaacaa agaagggaat gattgggatt aaagcgctcc agattaccag
     1621 cgacatcacc gccggacgcg ttgagtgcga catgatcttt ttattgaaga tgttgccaca
     1681 cgcccaacta aatgctgcag ccagggtcaa cataaagccg agcatcgcca catgctgacc
     1741 gttcagacta tcttcgatta acaccagtac gccaaaaatc gctaaggcga tccccgccaa
     1801 ttgtttgcca tgcagtcgct ccccgaaagt aaacgcgcca agcacgatag taaaaaacgc
     1861 ctgtgcctgt aacaccagcg aagccagtcc agcaggcata ccaaagttaa tggcgcagaa
     1921 cagaaaggca aactgcgcaa aactgatggt taatccatac cccaagagca aattcagtgg
     1981 tactttcggt cgtgcaacaa aaaagatggc cggaaaagcg accagcataa agcgcaaacc
     2041 ggccagcatc agcggtggca tgttatgaag ccccactttg atgaccacaa aatttagccc
     2101 ccatacgacc actaccagta gcgccaacac accatctttt cgcgacattc taccgcctct
     2161 gaatttcatc ttttgtaagc aatcaactta gctgaattta cttttcttta acagttgatt
     2221 cgttagtcgc cggttacgac ggcattaatg cgcaaataag tcgctatact tcggattttt
     2281 gccatgctat ttctttacat ctctaaaaca aaacataacg aaacgcactg ccggacagac
     2341 aaatgaactt atccctacga cgctctacca gcgcccttct tgcctcgtcg ttgttattaa
     2401 ccatcggacg cggcgctacg ctgccattta tgaccattta cttgagtcgc cagtacagcc
     2461 tgagtgtcga tctaatcggt tatgcgatga caattgcgct cactattggc gtcgttttta
     2521 gcctcggttt tggtatcctg gcggataagt tcgacaagaa acgctatatg ttactggcaa
     2581 ttaccgcctt cgccagcggt tttattgcca ttcctttagt gaataacgtg acgctggttg
     2641 tgctcttttt tgccctcatt aactgcgcct attctgtttt tgctaccgtg ctgaaagcct
     2701 ggtttgccga caatctttcg tccaccagca aaacgaaaat cttctcaatc aactacacca
     2761 tgctaaacat tggctggacc atcggtccgc cgctcggcac gctgttggtg atgcagagca
     2821 tcaatctgcc cttctggctg gcagctatct gttccgcgtt tcccatgctt ttcattcaaa
     2881 tttgggtaaa gcgcagcgag aaaatcatcg ccacggaaac aggcagtgtc tggtcgccga
     2941 aagttttatt acaagataaa gcactgttgt ggtttacctg ctctggtttt ctggcttctt
     3001 ttgtaagcgg cgcatttgct tcatgcattt cacaatatgt gatggtgatt gctgatgggg
     3061 attttgccga aaaggtggtc gcggttgttc ttccggtgaa tgctgccatg gtggttacgt
     3121 tgcaatattc cgtgggccgc cgacttaacc cggctaacat ccgcgcgctg atgacagcag
     3181 gcaccctctg tttcgtcatc ggtctggtcg gttttatttt ttccggcaac agcctgctat
     3241 tgtggggtat gtcagctgcg gtatttactg tcggtgaaat catttatgcg ccgggcgagt
     3301 atatgttgat tgaccatatt gcgccgccag gaatgaaagc cagctatttt tccgcccagt
     3361 ctttaggctg gcttggtgcc gcgattaacc cattagtgag tggcgtagtg ctaaccagcc
     3421 tgccgccttc ctcgctgttt gtcatcttag cgttggtgat cattgctgcg tgggtgctga
     3481 tgttaaaagg gattcgagca agaccgtggg ggcagcccgc gctttgttga tttaagtcga
     3541 acacaataaa gatttaattc agccttcgtt taggttacct ctgctaatat ctttctcatt
     3601 gagatgaaaa ttaaggtaag cgaggaaaca caccacacca taaacggagg caaataatgc
     3661 tgggtaatat gaatgttttt atggccgtac tgggaataat tttattttct ggttttctgg
     3721 ccgcgtattt cagccacaaa tgggatgact aatgaacgga gataatccct cacctaaccg
     3781 gccccttgtt acagttgtgt acaaggggcc tgatttttat gacggcgaaa aaaaaccgcc
     3841 agtaaaccgg cggtgaatgc ttgcatggat agatttgtgt tttgctttta cgctaacagg
     3901 cattttcctg cactgataac gaatcgttga cacagtagca tcagttttct caatgaatgt
     3961 taaacggagc ttaaactcgg ttaatcacat tttgttcgtc aataaacata cagcgatttc
     4021 ttcccgtttg cttaccctca tacattgccc ggtctgctct tccaatgacc acatccagag
     4081 gctcttcagg aaatgcgcga ctgacacctg ctgtcacggt aatgttgata tgcccttcag
     4141 aatgtgtgat ggcatggtta tcgactaact ggcaaattct gacacctgca cgacatgctt
     4201 cttcatcatt agccgctttg acaataatga taaattcttc gcccccgtag cgataaaccg
     4261 tttcgtaatc acgcgtccaa ctggctaagt aagttgccag ggtgcgtaat actacatcgc
     4321 cgattaaatg cccgtaggta tcattaacca atttaaatcg gtcaatatcc aacaacatta
     4381 aataaagatt cagaggctca gtgttgcgta actgatgatc aaaggattca tcaagaaccc
     4441 gacgccccgg caatcccgtc aaaacatcca tattgctacg gatcgtcagc aaataaattt
     4501 tgtaatcggt taatgccgca gtaaaagaaa gcaacccctc ctgaaaggcg tcgaaatgcg
     4561 cgtcctgcca gtgattttca acaatagcca gcattaattc ccgaccacag ttatgcatat
     4621 gttgatgggc agaatccatt agccgaacgt aaggtaattc atcgttatcg agtggcccca
     4681 gatgatcaat ccaccgacca aactggcaca gtccataaga atggttatcc gttatttctg
     4741 gcttactggc atctctcgcg accacgctgt gaaacatact caccagccac tggtagtggg
     4801 catcgatagc cttattgaga tttaacaaga tggcatcaat ttccgttgtc ttcttgatca
     4861 ttgccactcc tttttcacag ttccttgtgc gcgctattct aacgagagaa aagcaaaatt
     4921 acgtcaatat tttcatagaa atccgaagtt atgagtcatc tctgagataa cattgtgatt
     4981 taaaacaaaa tcagcggata aaaaagtgtt taattctgta aatcacctct gcattatcgt
     5041 aaataaaagg atgacaaata gcataaccca ataccctaat ggcccagtag ttcaggccat
     5101 caggctaatt tatttttatt tctgcaaatg agtgacccga acgacggccg gcgcgctttt
     5161 cttatccaga ctgccgctaa tgttgatcat ctggtccggc tgaacttctc gtccatcaaa
     5221 gacggccgca ggaataacga cattaatttc accgctctta tcgcgaaaaa cgtaacggtc
     5281 ctctcctttg tgagaaatca aattaccgcg tagtgaaacc gaagcgccat cgtgcatgtt
     5341 ttttgcgaaa tcaacggtca ttttttttgc atcatcggtt ccgcgatagc catcttctat
     5401 tgcatgaggc ggcggtggcg ctgcatcctg ttttaaaccg ccctggtcat ctgccaacgc
     5461 ataaggcatg acaagaaaac ttgctaatac aatggcctga aatttcatac tgactcctta
     5521 attgcgtttg gtttgactta ttaagtctgg ttgctatttt tataattgc
//
LOCUS       DAPHBJ010000243         5567 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.245, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000243 DAPHBJ010000000
VERSION     DAPHBJ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.245"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            68..409
                     /locus_tag="RHZ76_004157"
     CDS             68..409
                     /locus_tag="RHZ76_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814683.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDDFFTHICQLVALITIGLLFIGVLNAPINTYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            455..892
                     /locus_tag="RHZ76_004158"
     CDS             455..892
                     /locus_tag="RHZ76_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HDV2814684.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(934..1929)
                     /gene="wecH"
                     /locus_tag="RHZ76_004159"
     CDS             complement(934..1929)
                     /gene="wecH"
                     /locus_tag="RHZ76_004159"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HDV2814685.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAVLFVTGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLIKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            2104..2403
                     /locus_tag="RHZ76_004160"
     CDS             2104..2403
                     /locus_tag="RHZ76_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814686.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPPPEQKAQRVKVDPIRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            2498..3409
                     /gene="glyQ"
                     /locus_tag="RHZ76_004161"
     CDS             2498..3409
                     /gene="glyQ"
                     /locus_tag="RHZ76_004161"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDV2814687.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            3419..5488
                     /gene="glyS"
                     /locus_tag="RHZ76_004162"
     CDS             3419..5488
                     /gene="glyS"
                     /locus_tag="RHZ76_004162"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDV2814688.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFTEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
BASE COUNT         1415 a         1359 c         1428 g         1365 t
ORIGIN      
        1 tggcattttt cagttttgtc tttcgttggt tactcgtaat gtatcgctgg tagatatgga
       61 gatcgttatg aaaacctcaa agactgtggc aaaactatta tttgttgtcg gggcgctggt
      121 ttatctggtt gggctatgga tctcatgccc attgttaagt ggaaaaggct attttcttgg
      181 cgtgttaatg acagcaactt ttggcaacta tgcatatctt cgcgcagaaa aactcgggca
      241 actggatgat ttttttaccc atatctgcca gttagttgcg ttaatcacta tcggtctctt
      301 gtttatcggt gttttaaacg cacctatcaa tacttatgaa atggtgatct atcccatcgc
      361 cttttttgtc tgcttgtttg gtcaaatgcg tttgtttcgc tcggcatgag caacataaag
      421 ctcttacata tttaggaatg aaaggaatac tgtgatggac aacaaaatat caacctattc
      481 accggccttt agtattgtgt catggatagc tctcgttggt ggtatcgtta cctatctgtt
      541 agggctatgg aatgcagaga tgcagttaaa tgaaaaagga tattattttg ccgtactggt
      601 attaggactg ttttctgcgg cgtcttatca aaagaccgtt cgggacaagt atgaaggcat
      661 accgaccact tccatttatt atatgacctg cctgactgtc tttattatct ctgttgcatt
      721 actgatggta ggtctgtgga atgcgacatt attactcagc gaaaaaggtt tttatggact
      781 ggctttcttc ttaagcttgt ttggtgcagt agcggtgcag aaaaatattc gtgatgccgg
      841 aataaaccca ccaaaagaaa cacaggttac ccaggaagaa tacagcgaat aactcacgta
      901 agcccggtca gtccaatgtg accgggcttt tacttaactc actaatctgt ttctgtcgat
      961 tcgttgtacc agcatagaaa gtaacaaact cgctgccaac gtcgcgcaaa agatccaaat
     1021 aatatccagt attggccaat ttttaagctc aattccccgg gtgcgcagcg catggataat
     1081 caaggcgtgg aatccgtata tacccaacga atggcgtgag attaagccaa gtccgcgaat
     1141 ggtacgcgta tccagcgtgt ttttaatcag agtcaatagc gcgattgcgc agataaaaac
     1201 catcggccca cagtaaagat accaggtatc ggcaaaattt ccgcgccact gcaattcata
     1261 taatgtcccg cgagagataa taaaaacccc cgtcacaaac agtactgcgc tcacccacga
     1321 cagtgcttta tgctgcgtgt ccatcatccc tatagcgcgg cccaacatgc catacagaat
     1381 gtagtaaaaa gtatcgccat tgatatataa gttaattggc agccattcaa aaccgtcgat
     1441 tttctgcggc acggtgtttg ggttagcgat gataccaatc actaccatta gtaccagcaa
     1501 catttttccg ccgacgttct tcacctgaat cagcggtgaa accagataaa tcaccgcaat
     1561 cgcgaagaaa aaccataagt ggtaaaacac tggcttttgc agcaggtttt tcagcgctaa
     1621 ctccacattg atggaggtaa atagtgcaat gtagagcagc gcgattgcgc tataaaaaaa
     1681 cagacataag ccgatacgca agaaatggcg cggctgggcg ctgcgttcgc caaaaaagag
     1741 atagccggat atcatgaaaa atagcggcac gctgacacga gaggcagaat tcagaacatt
     1801 ggcgatatcc caggtgacgg ggctaacact atgagcattg gtcacatacc aggtagtggt
     1861 gtgaatcatc accaccatta aacacgctat ccctcgcagg ttatcaatcc agtaaatttt
     1921 gggctgcatc tgtgtctctg tatctggtta aaaaaagtct gaccgataaa tcattcagtt
     1981 ggcacactgg aataatctga gttttatcac tacagcttat agaggcttaa ggaaattcgt
     2041 aagatatcag ccactatacc gatataaata ataagactca cctgcaaacc agacggtaat
     2101 ttaatgatga tgaacgcttt ctttccggca atggcgctta tggtgctagt gggttgttct
     2161 acaccaccac cagaacagaa agctcaaagg gtgaaagttg atcccattcg gtcattgaat
     2221 atggaagcgt tatgcaagga tcaggcggca aaacgttata acaccggcga gcaaaaaatc
     2281 gacgtcaccg ccttcgaaca gttccaggga agctatgaaa tgcgcggtta taccttccgt
     2341 aaagagcagt ttgtctgttc ttttgacgcg gatggccatt ttttgcatct ttccatgcgt
     2401 taagccctgc tttttcccgt ttcgtactgt atatcttcca tccagcgggt atactgatcc
     2461 cttcctttaa atccacacgt atccagcacg aaataatatg caaaagtttg ataccaggac
     2521 cttccagggc ttgatcctga ccttacagga ttactgggct cgccagggct gcaccattgt
     2581 tcaaccattg gacatggaag tcggcgcggg aacctctcac ccaatgacct gtctgcgcgc
     2641 gctggggcca gaaccgatgg cggctgctta tgttcagcct tctcgtcgcc cgaccgatgg
     2701 tcgctacggc gaaaacccca accgtttaca gcactactat cagttccagg tggtcattaa
     2761 gccatcgccg gacaatattc aggagctgta cctcggttct ctgaaagagc tgggcatgga
     2821 cccgactatt cacgacatcc gtttcgtgga agataactgg gaaaacccga cgctgggtgc
     2881 ctggggactg ggctgggaag tgtggctgaa cggcatggaa gtgacgcagt tcacttactt
     2941 ccagcaggtt ggtggtctgg agtgtaaacc ggttaccggc gagatcacct acggtctgga
     3001 acgtctggcc atgtacattc agggcgtaga cagcgtttac gacctggtct ggagcgacgg
     3061 cccgctgggt aaaaccacct acggcgacgt gttccatcag aacgaagtgg agcagtccac
     3121 ttacaacttc gaatacgcgg atgtggactt cctgttcacc tgcttcgagc agtacgagaa
     3181 agaagcgcag cagctgctgg cgctggaaaa tccgctgccg ctgccagcct acgagcgtat
     3241 tctgaaagcc gcccacagct tcaacctgct ggatgcgcgt aaagccatct ccgtcaccga
     3301 gcgtcagcgc tacattctgc gcattcgcac cctgaccaaa gcagtggcag aagcatacta
     3361 cgcttcccgt gaagccctcg gcttcccgat gtgcaacaaa gataagtaag aggcggctat
     3421 gtctgagaaa acttttctgg tggaaatcgg cactgaagag ctgccaccaa aagcactgcg
     3481 cagcctggct gagtcctttg ctgcgaactt tactgcggag ctggataacg ctggcctcgc
     3541 acacggcacc gttcaatggt ttgctgctcc gcgtcgtctg gcgctgaaag tagctaacct
     3601 ggcggaagcg caaccggatc gtgaaatcga aaaacgcggc ccggcgattg cccaggcgtt
     3661 cgacgctgaa ggcaaaccga gcaaagcggc agaaggttgg gcgcgtggtt gcggtattac
     3721 cgttgaccag gctgagcgtc tgactaccga taaaggcgaa tggctgctgt atcgcgccca
     3781 tgtgaagggc gaaagcaccg aagcactgct gccgaatatg gttgcgactt ctctggcgaa
     3841 actgccgatc ccgaaactga tgcgttgggg cgcaagcgac gtgcacttcg tgcgtccggt
     3901 gcacaccgtg accctgctgc tgggcgacaa agtcattccg gcaaccattc tgggcattca
     3961 gtccgatcgc gtgattcgcg gccaccgctt tatgggcgag ccggaattca ccatcgataa
     4021 cgccgatcag tatccggaaa ttctgcgtga gcgtgggaaa gtcatcgccg attacgaaga
     4081 acgtaaggcg aagattaaag ccgatgccga agaagcagcg cgtaagattg gcggtaacgc
     4141 tgacttaagc gaaagcctgc tggaagaagt ggcttcgctg gtggagtggc cggtcgttct
     4201 gaccgcaaaa ttcgaagaga aattcctcgc ggtgccggct gaagcgctgg tttacaccat
     4261 gaaaggtgac cagaaatact tcccggtgta tgcgaacgac ggcaaactgc tgccgaactt
     4321 tatcttcgtt gccaacatcg aatcgaaaga tccgcagcag attatctccg gtaacgagaa
     4381 agtcgttcgt ccgcgtctgg cggatgccga gttcttcttc aacaccgacc gtaaaaaacg
     4441 tcttgaagat aacctgccgc gcctgcaaac cgtgttgttc cagcaacagt tggggacgct
     4501 gcgcgacaaa actgaccgca tccaggcgct ggctggctgg attgctgaac agattggcgc
     4561 tgacgttaac cacgctaccc gtgcgggtct gctgtctaag tgcgacctga tgaccaacat
     4621 ggtcttcgag ttcaccgaca cccagggcgt tatggggatg cactatgcgc gtcacgatgg
     4681 cgaagcggaa gatgtcgcgg tggcgctgaa tgagcagtat cagccgcgtt ttgctggtga
     4741 tgacctgccg tccaacccag tagcttgtgc gctggcgatt gctgacaaga tggataccct
     4801 ggcgggtatc ttcggtatcg gtcagcatcc gaaaggcgac aaagacccgt ttgcgctgcg
     4861 tcgtgccgcg cttggcgtgc tgcgaattat cgttgagaag aacctcaacc ttgatctgca
     4921 aacgctgacc gaagaagcgg tgcgtctgta tggcgataag ctgactaatg ccaacgtagt
     4981 tgatgatgtt atcgacttta tgctcggtcg cttccgcgcc tggtatcagg acgaaggtta
     5041 taccgttgac accatccagg cggtactggc gcgtcgtccg actcgtccgg ctgatttcga
     5101 tgcccgtatg aaagcggtat cgcatttccg taccctggat gcagctgctg cactggcggc
     5161 ggcgaacaaa cgtgtatcta acattctggc gaaatctgac gaagtgctga gcgaccgcgt
     5221 gaatgcctct accctgaaag agccggaaga aattaaactg gcgatgcagg ttgtggtgct
     5281 acgtgacaag ctggagccgt actttacgga aggtcgttac caggatgcgc tggtcgaact
     5341 ggctgagctg cgtgaaccgg ttgatgcttt cttcgataaa gtgatggtca tggttgatga
     5401 caaagaattg cgtatcaacc gtctgaccat gctggagaaa ctgcgcgaac tgttcctgcg
     5461 cgttgcggat atttcgctgt tgcaataata acgccgttat taaatagcct gccatctggc
     5521 aggctttttt tatcgctaaa taatacagca acctttaata atcttct
//
LOCUS       DAPHBJ010000244         5450 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.246, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000244 DAPHBJ010000000
VERSION     DAPHBJ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.246"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1943
                     /locus_tag="RHZ76_004163"
     CDS             <1..1943
                     /locus_tag="RHZ76_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HDV2814689.1"
                     /translation="NSIPKSRLAESVSKVNELARPQNNNFHDEMVEQYGRVKRFLPAV
                     LRDLHFQAAPAGEHTLSAIHYLTELNGSKKRILDNAPEHIITGPWKRLVYDAEGRIQR
                     AGYSLCLLERLQDALRRRDIWLENSDRWGNPREKLLQGEEWQAQRVPVCRALGHPTDG
                     HKGVQQLAVQLDETWKTVASRFEGNAEVHICHDGKHPSLTISSLEKLEEPPSLHRLNS
                     RVRLLLPPVDLTELLLEIDARTGFTREFTHVSESGARAQDLHISLCAVLMAEACNIGL
                     EPLIKHNIPALTRHRLSWVKQNYLRAETLVSANARLVDFQSTLELAGRWGGGEVASAD
                     GMRFVTPVKTVNSGPNRKYFGSGRGITWYNFVSDQYSGFHGIVVPGTLRDSIFVLEGL
                     LEQQTGLNPVEIMTDTAGTSDIIFGLFWLLGYQFSPRLADAGEAVFWRVDKSANYGAL
                     DELARGCADLSKAEDQWDEMMRTAGSLKLGTIHASELIRSLLKSSRPSGLAQAIMEVG
                     RVNKTLYLLNYIDDEDYRRRILTQLNRGEGRHAVARAICYGQRGEIRKRYREGQEDQL
                     GALGLVTNAVVLWNTLYMQEALSWIRSNGEEIGVEDIARLSPLMHGHINMLGHYTFTL
                     PEDILKGELRELNFNLNNELTS"
     gene            1979..2773
                     /locus_tag="RHZ76_004164"
                     /pseudo
     CDS             1979..2773
                     /locus_tag="RHZ76_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008815934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein RepA"
     gene            3346..3588
                     /locus_tag="RHZ76_004165"
     CDS             3346..3588
                     /locus_tag="RHZ76_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDV2814690.1"
                     /translation="MARTTSVTIGEQLDSFISRLIDSGRYGSASEVMRSALRLLEQQE
                     TNDEVIRQAVIAGLESGESSLSLRDIAAQRKLRHRV"
     gene            3581..3865
                     /locus_tag="RHZ76_004166"
     CDS             3581..3865
                     /locus_tag="RHZ76_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV2814691.1"
                     /translation="MYKLTERAADDFAGIYDYTLLKFGEAQADHYTDALEAFFETLAG
                     MPDMGRDYHTVPGVMRIEFQRHAIFYTVRDTDILIARILHQQMNHKRHLL"
     gene            complement(4003..4095)
                     /locus_tag="RHZ76_004167"
                     /pseudo
     CDS             complement(4003..4095)
                     /locus_tag="RHZ76_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001436758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA partition complex ParG"
     gene            complement(4601..4933)
                     /locus_tag="RHZ76_004168"
                     /pseudo
     CDS             complement(4601..4933)
                     /locus_tag="RHZ76_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014594796.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
     gene            complement(4933..>5450)
                     /gene="glgC"
                     /locus_tag="RHZ76_004169"
     CDS             complement(4933..>5450)
                     /gene="glgC"
                     /locus_tag="RHZ76_004169"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462437.1"
                     /note="catalyzes the formation of ADP-glucose and
                     diphosphate from ATP and alpha-D-glucose 1-phosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDV2814692.1"
                     /translation="SCVQSDPESEPYWRDVGTLEAYWKANLDLASVTPELDMYDQDWP
                     IRTQHMESLPPAKFVQDRSGSHGMTLNSLVSGGCIISGSVVVQSVLFPRVRVNSFCNI
                     DSAVLLPEVWVGRSCRLRRCIIDRACVIPEGMVIGENAEEDARRFYRSEEGIVLVTRE
                     MLRKLQIKQER"
BASE COUNT         1366 a         1382 c         1431 g         1271 t
ORIGIN      
        1 tcaacagcat accgaaaagc aggctggcag aatccgtcag caaggtaaat gagctggccc
       61 ggcctcagaa caacaatttc catgacgaaa tggtggagca gtacgggcgg gtaaagcgct
      121 ttcttccggc ggtattgcgc gacctgcatt tccaggcagc gccagccgga gaacatacgt
      181 tgtccgccat tcattatctg accgaactga acggctcgaa aaagcgcatc ctggacaatg
      241 cgcctgaaca tattattacc ggcccctgga aacgcctggt atacgatgcg gagggccgga
      301 tacagcgtgc cggttactcg ctttgtctgc tggagcgcct tcaggatgca ctacgtcgaa
      361 gggacatctg gctcgaaaac agcgatcgct ggggaaatcc ccgcgagaag ctgttgcagg
      421 gggaagaatg gcaggctcag cgggtccccg tttgccgggc gctgggacat cccactgatg
      481 gacataaagg cgtgcaacag ttggcggtcc agctagatga aacctggaaa accgtcgcat
      541 cccgctttga aggaaatgcg gaggttcata tttgccatga cggtaaacat ccttccctga
      601 ctatcagcag cctggagaaa ctggaggagc caccatcgtt gcatcgtctc aacagtcggg
      661 taaggctgct actcccgcca gtagatttga cggaactgtt gcttgaaata gatgccagaa
      721 cgggatttac acgtgagttt acgcatgtca gtgaatccgg ggcccgggca caggatctgc
      781 acatcagcct gtgcgcggtc ctgatggcag aggcctgcaa tatcgggctg gaaccgctga
      841 taaagcacaa tataccggcg ctgacgcgcc accggcttag ctgggtaaaa cagaattacc
      901 tccgggcaga aacgctggtc agcgccaatg cccgcctggt tgattttcag tccacactgg
      961 agcttgctgg ccgctggggt ggtggcgaag tggcttcagc tgatggcatg cgctttgtca
     1021 cgccggtgaa aaccgtcaat tcaggaccta acagaaaata ttttggttcc ggacgtggca
     1081 tcacctggta caacttcgtc tctgatcagt actctggatt ccacggcatc gttgtccccg
     1141 gcacattacg agattccatt tttgtgctgg aaggccttct ggagcagcag acagggctga
     1201 atccggttga gatcatgaca gacacagccg gtaccagcga cattattttt ggcctcttct
     1261 ggctgctggg ataccagttt tccccccggc ttgccgatgc cggtgaagcg gtattctggc
     1321 gagtggataa atcggcaaat tacggtgcac tggacgaact ggcacgtggt tgtgccgatc
     1381 tgtcgaaagc cgaggatcag tgggatgaga tgatgcgaac cgccggttcg ctgaaactgg
     1441 gcaccattca tgcttcagaa ctcattcgct ctttgctgaa aagctcgcgc ccatcagggc
     1501 tggcacaggc gatcatggaa gtggggcgcg tcaacaagac gctgtacctt cttaattata
     1561 ttgatgatga ggattatcgt cggcggatcc tgacgcagct aaaccggggg gaaggccgcc
     1621 atgctgtggc gagggcgatc tgctacgggc agcgcggtga gatcagaaag cgctatcgtg
     1681 aaggtcagga agatcagctg ggggcactgg gcctggtcac taacgcagtg gtactgtgga
     1741 acacacttta tatgcaggaa gccctgagct ggatacgcag taatggagaa gaaatcgggg
     1801 ttgaagatat cgcccggttg tccccactga tgcacgggca tatcaatatg ctggggcatt
     1861 atacgtttac gctaccggag gatattttga agggggaact gagagaacta aatttcaatt
     1921 taaacaatga attaacttct tagcgtacgt tttcgttcca ttggccctca aaccccgtaa
     1981 acagcatggt cgagccttac gtaaccttat gagccaggac aaaacctctg gtgcctgggt
     2041 tattcgtcgt cgtgtttctg ccgatccaat cttcactttt ttgcgacctg tcgcggacag
     2101 aaaaagagct ttccgtgaag tacgccggcg tttgctcgat gcattgtttg tgcttttcgt
     2161 caacaaagcc gatcttgcta ccggtattgt cactatcaac atcactaagc tggctgaaga
     2221 actcagtcca cggaatgaag atggccagat catccctgaa actgccgtga cggtatccag
     2281 ggtgtcgcgt ctgatagacg aattggcgag gtttggcata gtccttgcgc ctgaaactga
     2341 gtgggattat gtgaacgggt gccgttttcc taagcatata atcattacag aagaaggctg
     2401 gcgtcttacc ggtgtggata tggacaagct acgagccgag caagaagaac gcctacgcgc
     2461 tattgaggac ggcattctac aacccggtga ggctatgaca gtaaaagaag cccgtaagcg
     2521 ctggtatgaa cgttgccgcc atcagacgat actcagccgc cgtacacgcg ctattgaggg
     2581 caaacaacgg cgtaagctgg ccgaattacc gttcgatgag cgaaagcgtc aggtggccga
     2641 gcgtattttc cgtgatatga agggtgatat acaccatcta acccctcagc agtttgaaaa
     2701 aatggtctgg actcagcttt accagcttga actggtaaat atggagcagc caggcacagc
     2761 cccgcctcac taacccgccc tctttttact aatccgattc cctgcggagg atgttcgttg
     2821 tccatctgct aaaacagctc gttttttagt caccattatc acgataacct caccgatatt
     2881 tggtcaaaac cccaatcaac accggcgtta tatcttttat gcacacttac ctgcaaatag
     2941 gtaaatggga ctatctgcaa tcaaccaata gttagctgca aaacaatcag gtcttcgacc
     3001 tacttaactt cattaaagat ctacttattc gctggataag ttctgtggat aacggggaaa
     3061 accgataatg accgcccttg cagcggccag atggccgcgc cggttcggca tggtcgctat
     3121 gctccccaaa agactttaag tagcacctcc ggccttagcc ggaggcatgg agtcaacgag
     3181 acgcaatgca gcgaaaaggc gcttttcgcc cttacctact tcatcgctaa aagtaagcta
     3241 aaaaaccagt ctggcaggaa gggggcgaaa tccgtttggg tgtattcgtt gacaccagga
     3301 gcaagtattc ctatcattgt gttgatagtt aacacagggg gcgttatggc cagaacaaca
     3361 agcgttacga ttggcgaaca actcgattcg tttattagtc gattaataga cagtggccgt
     3421 tacggctctg cgagtgaagt tatgcgttct gcgctgcgat tgctggagca acaggaaaca
     3481 aacgatgaag ttattcgtca ggctgttatt gctgggctgg aaagcggtga aagctcgcta
     3541 tcactgcggg atattgcagc acaaagaaaa ctgagacatc gtgtataaac tcactgaacg
     3601 ggcagcagac gacttcgccg ggatttatga ctatacgctc ttgaaatttg gtgaagctca
     3661 ggcagaccat tatacagacg cacttgaggc atttttcgag acactggccg gaatgccaga
     3721 tatgggacgt gactaccata ccgttcctgg tgtgatgcgc attgaatttc agcgacatgc
     3781 cattttttat acggttcgtg atacggatat tctgattgcg cgtattttgc accaacagat
     3841 gaaccataaa cgccatttac tgtaagtcta aacacgctgc cgcgctttgc ttggccaaca
     3901 ccgatctggc acataaaaag tttatgcacc agatcagtgt tctcggtaag gataggtgtg
     3961 tttttgagtt aatgtgtttt tactcatttg agtcttttgg ctgtcatttt ctttcagcca
     4021 attatcgacc aagtccttta tcacatcagt aatagatgtg ccttgttttg tactcgcagc
     4081 tttgaagcgg atatgtatcc taggccgcct ttcgagtatc ataggggtca gtttggatat
     4141 cgaaaattat tgtactataa ggacttcccc ggcatgtcaa agccggtatt tatggtttct
     4201 gtctgctctt cagtaaaaat aaaggggaga gactgcggta ctataaacgg ccatgatgaa
     4261 tcgtcatcga ttcgggccct gatgaaagac gcgcgtgagg ttcttgttcg ttaagcggat
     4321 acgtatatcc tgtacaatag ccagataggc ctcacggggt ctaacccttt atcatcaacg
     4381 aattgcaggt cagatggtta tacagcttcg agctttagtt gactgagtac agccggaaat
     4441 catcaccgta atgtagtgat gcccaacaca gccgcgcatt tttggcggcg atggccacaa
     4501 cagcccgcca gtaccctctg cgctcaacca acatacaaac ccaacggctg aatgagtcag
     4561 tccttttccc ggcgccaata agcactgaac gagccccctg aaccagaagc gttcgcagat
     4621 acgaatcacc ggccaggtta gtgtcgtgat acgggctacc ggggcggtca tagagatgcg
     4681 gcgcgtcaat caggtaaata cccacgccgt tgaaatgccc gaacagcagc gtgatacgtc
     4741 cggcgaaggt atcccgacgc gttacaacct gcgcatcggg aatgccgcga cgaatatcag
     4801 gaaacgccgg gagcagcacg cgggcatcca cgccgtccgc tatttgcgct gcgggtaacg
     4861 ccccaataac atccgccagt cccccggttt ttaacagggg gaacatctct gaacaaacgt
     4921 gtaaaacctg cattatcgct cctgtttgat ctgcagtttg cgtagcattt cacgcgtgac
     4981 cagcacaata ccttcctctg aacggtagaa acgacgcgca tcttcttccg cgttttcacc
     5041 aatcaccatg ccttcgggaa taacgcaggc gcggtcaata atgcaacgac gtaagcggca
     5101 cgaacgtccc acccacactt caggtaacaa cactgccgaa tcaatgttac agaatgaatt
     5161 cacccgcact cgcgggaaca gaacggactg caccaccacc gaacctgaaa ttatgcatcc
     5221 gccagaaacc agcgagttca gcgtcattcc gtggctgccg gaacggtcct gaacaaattt
     5281 cgccggtggc agcgattcca tgtgctgcgt acggattggc cagtcctggt cgtacatgtc
     5341 cagttcaggc gtcaccgaag ccagatcgag gttcgccttc cagtaggctt ccagcgtacc
     5401 cacgtcacgc cagtacggct cggattccgg gtcagactgc acgcaggaca
//
LOCUS       DAPHBJ010000245         5441 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.247, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000245 DAPHBJ010000000
VERSION     DAPHBJ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.247"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..4511
                     /gene="sslE"
                     /locus_tag="RHZ76_004170"
     CDS             <1..4511
                     /gene="sslE"
                     /locus_tag="RHZ76_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HDV2814693.1"
                     /translation="SATLLAGCDGGGSGSSSDTPPVDSGTGSLPEVKPDPTPNPEPTP
                     EPTPDPEPTPEPIPDPEPTPEPEPEPVPTKTGYLTLGGSQRVTGATCNGESSDGFTFK
                     PGEDVTCVAGNTTIATFNTQSEAARSLRAVEKVSFSLEDAQELAGSDDKKSNAVSLVT
                     SSNSCPANTEQVCLTFSSVIESKRFDSLYKQIDLAPEEFKKLVNEEVENNAATDKAPS
                     THTSPVVPVTTPGTKPDLNASFVSANAEQFYQYQPTEIILSEGRLVDSQGYGVAGVNY
                     YTNSGRGVTGENGEFSFSWGETISFGIDTFELGSVRGNKSTIALTELGDEVRGANIDQ
                     LIHRYSTTGQNNTRVVPDDVRKVFAEYPNVINEIINLSLSNGATLGEGEQVVNLPNEF
                     IEQFNTGQAKEIDTAICAKTDGCNEARWFSLTTRNVNDGQIQGVINKLWGVDTNYKSV
                     SKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKNYWLAFGEKRAWDKNEL
                     AYITEAPSIVRPENVTRETATFNLPFISLGQVGDGKLMVIGNPHYNSILRCPNGYSWN
                     GGVNKDGQCTLNSDPDDMKNFMENVLRYLSNDRWLPDAKSNMTVGTNLDTVYFKKHGQ
                     VTGNSAAFGFHPDFAGISVEHLSSYGDLDPQEMPLLILNGFEYVTQVGNDPYAIPLRA
                     DTSKPKLTQQDVTDLIAYMNKGGSVLIMENVMSNLKEESASGFVRLLDAAGLSMALNK
                     SVVNNDPQGYPDRVRQRRSTPIWVYERYPAVDGKPPYTIDDTTKEVIWKYQQENKPDD
                     KPKLEVASWQEEVEGKQVTQFAFIDEADHKTPESLAAAKKRILDAFPGLEECKDSDYH
                     YEVNCLEYRPGTGVPVTGGMYVPQYTQLSLNADTAKAMVQAADLGTNIQRLYQHELYF
                     RTNGRKGERLSSVDLERLYQNMSVWLWNKIEYRYENDKDDELGFKTFTEFLNCYANNA
                     YDGGTQCSAELKQSLIDNKMIYGEGSKAGMMNPSYPLNYMEKPLTRLMLGRSWWDLNI
                     KVDVEKYPGAVSAEGEEVTETINLYSNPTKWFAGNMQSTGLWAPAQQEVSIKSNAKVP
                     VTVTVALADDLTGREKHEVALNRPPRVTKTYSLDASGTVKFKVPYGGLIYIKSDSKEE
                     KSANFTFTGVVKAPFYKDGKWKNDLKSPAPLGELESASFVYTTPKKNLEASNYKGGLK
                     QFAEDLDTFASSMNDFYGRDGESGKHRMFTYEALTGHKHRFTNDVQISIGDAHSGYPV
                     MNSSFSPNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGATEVANNVLALYMQDRYLG
                     KMNRVADDITVAPEYLEESNGQAWARGGAGDRLLMYAQLKEWAEKNFDIKQWYPEGSL
                     PAFYSEREGMKGWNLFQLMHRKARGDDVGNDKFGNRNYCAESNGNAADTLMLCASWVA
                     QTDLSAFFKKWNPGANAYQLPGATEMSFEGGVSQSAYNTLASLDLPKPKQGPETINKV
                     TEYSMPAE"
     gene            4651..>5441
                     /gene="pppA"
                     /locus_tag="RHZ76_004171"
     CDS             4651..>5441
                     /gene="pppA"
                     /locus_tag="RHZ76_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HDV2814694.1"
                     /translation="MLFDVFQQYPAAMPVLATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSAQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASVIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGALSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATL"
BASE COUNT         1364 a         1333 c         1521 g         1223 t
ORIGIN      
        1 tgagcgcaac cctgttagcc ggttgtgatg gcggtggttc cggatcttcc tccgatacgc
       61 cgcctgtaga ttctggaaca gggtctttgc cggaagtgaa acctgatcca acaccaaacc
      121 cggagccgac gcctgagcca acgccggacc cagagcctac gccagaaccg atacctgatc
      181 ctgaaccaac accagaaccg gagccagaac ctgttcctac gaaaacgggt tatctgaccc
      241 tgggcggaag ccagcgggta actggtgcta cctgtaatgg tgaatccagc gatggcttta
      301 catttaaacc tggcgaggac gttacttgcg tggcgggtaa cacgacaatt gccaccttca
      361 acactcagtc agaagctgcg cgtagcttgc gtgcggttga aaaagtgtcg tttagccttg
      421 aggacgcgca agaactggcg ggctccgatg acaagaaaag caatgcggtt tcgctggtaa
      481 cgtccagtaa cagctgtccg gcgaatacag aacaggtttg tctgacgttc tcctcggtga
      541 tcgagagtaa acgcttcgac tcgctgtata agcaaatcga tctggcaccg gaagagttca
      601 aaaagctggt caatgaagag gtggaaaaca atgctgcgac cgataaagcg ccatccactc
      661 atacttcacc ggtcgtgccc gtcaccacgc cgggaacaaa accggatctg aacgcttcct
      721 tcgtgtcggc taacgcggaa cagttttatc agtatcaacc cactgaaatc attctctctg
      781 aaggtcgact ggtcgatagc cagggatatg gtgttgctgg cgtcaactac tacaccaatt
      841 caggccgtgg cgtgacaggg gaaaatggtg aattttcctt tagctggggc gaaaccatct
      901 cctttggtat cgataccttt gaactgggtt cagtgcgcgg caataagtcg accattgcgc
      961 tgactgaact gggtgatgaa gttcgcgggg cgaatattga tcagcttatt catcgctatt
     1021 cgacgaccgg gcaaaataat acccgtgttg ttccggacga tgtacgcaag gtctttgccg
     1081 aatatcccaa cgtgatcaac gagattatca atctctcgtt atccaacggt gcgacgctgg
     1141 gggaaggtga gcaagtcgtt aatctgccta acgaatttat tgagcagttt aatacgggtc
     1201 aggccaaaga gatcgatacc gcgatttgtg cgaaaaccga tggttgtaac gaggctcgct
     1261 ggttctcgct gacgacgcgc aatgttaatg acggccagat tcagggcgtt atcaacaagc
     1321 tgtggggcgt ggatacgaac tacaaatctg tcagcaagtt ccatgtattc catgactcca
     1381 ccaacttcta tggcagcacg ggtaatgcgc gcggtcaggc ggtggtgaat atctccaacg
     1441 cggccttccc gattctgatg gcgcgtaatg ataaaaacta ctggctggcc ttcggcgaga
     1501 aacgggcctg ggataaaaat gagctggcgt acattactga agcgccttcc attgtgcgac
     1561 cagagaacgt gacacgcgaa accgccacct tcaacctgcc gtttatttcg ctggggcaag
     1621 tgggcgatgg caagctgatg gttatcggta acccacacta caacagcatc ctgcgttgcc
     1681 cgaacggtta cagctggaac gggggcgtta ataaagatgg gcagtgtacg ctcaacagcg
     1741 acccggatga catgaagaac ttcatggaga acgtgctgcg ctatctgtca aatgatcgct
     1801 ggttgccgga tgcaaaatcc aatatgaccg tgggtactaa cctggacacg gtgtatttca
     1861 aaaaacacgg gcaggttaca ggaaatagtg ctgcgttcgg ctttcatccg gattttgcgg
     1921 gtatctctgt tgagcattta agtagctatg gcgatctcga cccgcaggaa atgccgctgc
     1981 tgatcctcaa cggctttgag tatgtgactc aggttggtaa cgatccttat gcaatcccgc
     2041 tgcgtgcaga taccagcaaa ccgaagctga cccagcagga tgtgaccgat ttgatcgcct
     2101 atatgaacaa aggtggatcg gtgctgatca tggaaaacgt gatgagcaat cttaaggaag
     2161 agagcgcatc tggctttgta cgtctgcttg atgccgcagg tttgtcgatg gcgcttaaca
     2221 agtcggtagt aaataacgat ccgcaaggct acccggaccg cgttcgtcaa cgacgttcaa
     2281 cgccaatttg ggtctatgag cgttatccgg ctgtcgatgg taaaccaccg tataccattg
     2341 atgacaccac gaaagaagtt atctggaaat atcagcaaga aaacaaacct gatgacaaac
     2401 cgaagctgga agttgccagc tggcaggaag aagttgaggg taaacaggta actcaattcg
     2461 cctttatcga tgaagccgac cacaaaacgc ctgagtcact ggctgcggcg aagaagagaa
     2521 ttctggacgc gttcccaggg ctggaagagt gtaaggattc tgactaccac tatgaggtca
     2581 actgtctgga atatcgtcct ggcacggggg ttccggttac tggtggcatg tatgttccac
     2641 agtatacgca actaagcctt aacgccgaca ctgcgaaagc gatggtgcag gctgcggatt
     2701 taggcaccaa cattcagcgt ctgtatcagc atgagcttta cttccgtacc aatggtcgca
     2761 aaggtgagcg tctgagcagc gtcgatctgg aacgtctgta ccagaacatg tcggtctggc
     2821 tgtggaataa aattgaatat cgctatgaaa acgacaagga tgacgagctg ggctttaaaa
     2881 cgttcaccga gttcctgaac tgttacgcca acaatgctta tgatggtggc acgcagtgct
     2941 ccgcagagct gaaacaatcg ctgatcgata acaagatgat ctacggtgaa ggcagcaaag
     3001 cgggcatgat gaacccgagc tacccgctta actatatgga aaaaccgctg acgcgcctga
     3061 tgctggggcg ttcctggtgg gatctgaaca tcaaggttga tgtcgagaag tatccggggg
     3121 cggtatcggc tgaaggtgag gaggttactg aaaccatcaa cctgtactcg aatccgacca
     3181 aatggtttgc gggtaacatg cagtctactg gcctgtgggc tccggctcag caggaagtca
     3241 gcattaagtc caatgcgaaa gtccctgtga ctgttaccgt ggcgctggct gacgacctga
     3301 ccgggcgtga gaagcatgag gttgcgctga accgtccgcc aagagtgact aaaacatact
     3361 ctctggatgc tagcggcacg gtgaagttca aggttcctta cggtggtctg atttatatca
     3421 agagcgacag taaagaggag aaatcagcca acttcacctt tactggcgtg gtaaaagcgc
     3481 cgttctataa agacggtaaa tggaaaaacg acctgaaatc ccctgcgccg ttgggtgagc
     3541 tggagtctgc gtcgttcgtc tataccacgc cgaagaagaa ccttgaggcc agcaattaca
     3601 agggcggtct gaaacaattc gctgaggatc tggatacctt tgccagctcg atgaatgact
     3661 tctacggtcg tgatggcgaa agcggtaagc accggatgtt tacctatgaa gcattgacgg
     3721 ggcacaaaca tcgtttcacc aacgatgtgc agatctccat cggtgatgcg cactctggtt
     3781 atccggtgat gaacagcagc ttctcgccga acagcaccac gctgccgacg acgccgctga
     3841 acgactggct gatctggcac gaagtagggc acaacgctgc agaaacgccg ctgactgtac
     3901 cgggcgcaac tgaagtggcg aacaacgtgc tggcgctgta catgcaggat cgttatctcg
     3961 gcaagatgaa ccgtgtcgct gacgatatta ccgttgcgcc ggaatatctg gaggagagca
     4021 acggtcaggc atgggcgcgt ggcggtgcgg gtgaccgtct gctgatgtac gcgcagctga
     4081 aggaatgggc agagaaaaac tttgatatca aacagtggta tccagaaggc tctctgccag
     4141 cgttctacag cgagcgtgaa gggatgaaag gctggaacct gttccagttg atgcaccgta
     4201 aagcacgcgg cgatgatgtt ggcaatgaca aatttggcaa cagaaactac tgtgccgaat
     4261 ccaacggtaa cgctgccgac acgctgatgc tgtgtgcatc ctgggtcgct cagacggacc
     4321 tttccgcatt ctttaagaaa tggaatccgg gcgcgaatgc ttaccagttg ccgggagcga
     4381 cagagatgag cttcgagggc ggtgtgagcc agtcggctta caacacgctc gcgtcactcg
     4441 atctgccgaa accgaagcaa gggccggaaa ccattaacaa ggttaccgag tattcgatgc
     4501 ctgctgaata acccggataa ggcgttcacg ccgcatccgg catgaaataa ggcgcaccgg
     4561 gttgcgccag cttatcgggc ctggaggaaa gccatatggc atacacctgc aggcccgcat
     4621 ccggcaagtt acaacaaaca acctttaacc atgctttttg atgtttttca gcaatacccc
     4681 gcggcgatgc ccgtcctggc aaccgtcgga ggattgatta taggtagttt tttgaatgtg
     4741 gtgatttggc gttaccccat catgctgcgc caacaaatgg cggagtttca cggtgaaatg
     4801 ccgagtgcgc agtcaaaaat aagcctggcg ctgccacgtt cgcactgtcc acattgtcag
     4861 cagaccatcc gcgttcgtga caatattccg ctgctctcct ggctgatgct caaagggcgc
     4921 tgccgcgact gtcaggcgaa aatcagcaag cgttatccgc tggtggagtt attgacagca
     4981 cttgcttttt tgctggcgag tctggtctgg ccagaaagtg gatgggggct ggcggtgatg
     5041 atattatccg cctggctgat tgccgcgagc gtcatcgacc tcgatcacca atggctgccc
     5101 gatgttttta ctcagggcgt attgtggaca ggactgattg cggcatgggc gcagcagagt
     5161 ccgctcacgc tacaagatgc agtcaccggc gtcctggtgg gatttatcac tttttactct
     5221 ctgcgttgga tagccggaat agttctgcgt aaagaagcat taggcatggg cgatgtatta
     5281 cttttcgccg cgttaggtgg ctgggtgggg gcattgtcgc tgcccaatgt tgctttaatc
     5341 gcctcatgct gcggcctgat atatgccgtt attacaaaaa gaggatcaac cacactgcct
     5401 tttggaccgt gtttaagtct gggcggtata gcaacacttt a
//
LOCUS       DAPHBJ010000246         5411 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.248, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000246 DAPHBJ010000000
VERSION     DAPHBJ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.248"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(113..1396)
                     /gene="aroA"
                     /locus_tag="RHZ76_004172"
     CDS             complement(113..1396)
                     /gene="aroA"
                     /locus_tag="RHZ76_004172"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDV2814695.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTGLGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKSTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1467..2555)
                     /gene="serC"
                     /locus_tag="RHZ76_004173"
     CDS             complement(1467..2555)
                     /gene="serC"
                     /locus_tag="RHZ76_004173"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HDV2814696.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2754..3446)
                     /locus_tag="RHZ76_004174"
     CDS             complement(2754..3446)
                     /locus_tag="RHZ76_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDV2814697.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3576..5336
                     /locus_tag="RHZ76_004175"
     CDS             3576..5336
                     /locus_tag="RHZ76_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HDV2814698.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1387 a         1404 c         1283 g         1337 t
ORIGIN      
        1 acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc cgaaaaaaaa cgccgacaat
       61 gtgccgacgt tttgataaag aaagatttgg ctatttattg cccgttgttc attcaggctg
      121 cctggctaat ccgcgccagc tgctcgaaat aatccggaaa tgttttggcc gtgcatttgg
      181 gatcaagaat cgtcactggt gtatctgaca acgccaccag cgagaaacac atcgccatcc
      241 ggtgatcatt gtatgtcgcg atctcggcaa agttcagttt ttccggtgga gtgatacgaa
      301 tgtaatcgtg cccctcttct acttccgcac cgactttacg cagttctgtt gccatcgcaa
      361 acaggcgatc ggtttcttta acacgccagt tatagatatt gcgcagcgtg gtggtgcttt
      421 ttgcaaataa cgccgccgtg gcaatggtca tcgccgcatc gggaatatgg ttcatatcca
      481 tatcaatagc gttcagttca ccacgcgtgc aggaaatata atcatcgccc cagcaaatgg
      541 tcgcgcccat tttttccagc acatcagcaa agcgaatatc accctgcata ctgttacgtc
      601 caataccggt cacttttaca gtgccgcctt tgattgctgc tgctgccaga aagtaagaag
      661 ccgaagatgc atcgccttcg accaaataag tacccggaga ctgataagac tgcccgcctt
      721 ttacgacaaa ttgttgatag tgctgatttt caatttcaac accaaacgtc ttcatcagat
      781 tgagtgtgat gtcgatataa ggtttagaaa ccagatcgcc tttaatacga atcaccgtat
      841 cttccggcgc aagaggcgca gtcattaaca gtgcggtgag gaattggctg gaaacggagc
      901 catcaacgtc aacgttgccg ccggtaaagc cgccctgtaa acgcaacggc ggataatttt
      961 cttgttccag gtaagtgatc ttcgccccgc ccaggcgcag agcatccacc agatgaccaa
     1021 tcgggcgttc tttcatacgc ggctcaccgg tcagcacaat atcattgcta cccagacaaa
     1081 gagctgccgc cagcggacgc attgccgttc cggcgttacc gaggaacaac tccagggcac
     1141 cttctgcgtg taatggaccg ccgttaccga taatttcgca acgcgtacga tcggctgaaa
     1201 gcgtatagct tacccctaac cctgttaatg cattcagcat atggcgcacg tcatcgctat
     1261 ccagcagatt ggttaatact gttttgccgt gtgctaatgc cgccagcaat aaagcgcggt
     1321 tagaaacgct cttggaaccg ggcagattaa tagtgccatc gacacgagcg atgggttgta
     1381 acgtcaggga ttccatgaac tcaactctct acaacagaaa taaaaacccc acaggctggc
     1441 tgtggggatt aagcaaaatt tcggcattag ccgtgacggc gttcgaactc aaccatgaag
     1501 tctgtcagcg ctttaacgcc ttccagcggc atggcgttat aaatagacgc gcgcattccg
     1561 ccgaccacac ggtgaccttt cagtgcatga aggccagcag caaaagactc ttcaaggaac
     1621 aatttgtcaa gcgcactgtc cgccaactgg aacggcacgt tcatccgcga acggttagct
     1681 ttcgccacgt cattgcggta gaaatcgctg ttatcaatca ccccatatag cagttctgct
     1741 ttttgctgat tgattttatc catttcagct acaccgccgt tcgctttcag ccatttaaag
     1801 accagaccag atagatacca ggcaaatgtc ggcggcgtgt taaacatgga gccgttatcg
     1861 ttgaggatgg aataatccag aatcgacgga cacgcgatat tcgctttgcc cagcaaatct
     1921 tcacgaacga tgacgattgt caggccagcc gggccgatat ttttctgcgc gccagcgtaa
     1981 attacaccat aacggctgac gtcaatcgga cgggaaagaa tggttgaaga gaagtcggcg
     2041 gcgaccacca catctttgcc gaagtctggc gtctcgtcga tggcgatacc gtcgatggtt
     2101 tcattcgggc agtaatgcat ataagccgca ttatcagaga gttgccattc acgcattggc
     2161 ttaaccgcgc gcagaccatc aacagtcact ttggcgtcaa agacattagg cgtgcagtat
     2221 tttttcgctt ctttaatggc acttgccgcc cagtaaccgg catcaacata atctgcggtg
     2281 gttttatcac cgagaatatt cagcggtacc gcagcaaact gaccgcgacc accgccatgg
     2341 cagaataata ccttgtagtt ggaggggaca ttaagaagat cgcgaaaatc cttctcggct
     2401 tcctctgcaa cctgaatgaa ctctttgcca cggtgactca cttccatcac cgacgtacca
     2461 agaccgttcc agtcgcgcag ttcctgttga gcctgtttaa gcacctctgc cggtagcatt
     2521 gccggaccag aactaaaatt gaagatttga gccatttccc ctcaccacgt tgcgttgcta
     2581 ttaacccgcg gtcaatcgat tgcgaccaca ggtaatacat tattcctgtg gctatcggtt
     2641 ttatcattca gtgacacaca tcgcaatgag taaaaccgtt gagggcaaaa atgtggcttg
     2701 catcacacaa aagaatctct cggattatta gcaaaaagcc gatgttttta ctgtcaggtc
     2761 acccgttttg cccgacttgc cttcgtccat tctggatttg cacagcgcgg acataattgt
     2821 ttttcccccg ctttcatatg aatgatttca ctacaacgaa tacaggcata gtccgccgac
     2881 tccggcacca ctttgtaacg ctgcgtacaa tcactgggta aaataagtaa acccggtttc
     2941 accttgtcat cttcaaagaa atagacactg atttgcccgt tggtttcgag aatcgccaga
     3001 cgtacctgcc ccagctgctc cacgccacgc aatcgtagct ccataaagaa ctcaaattcc
     3061 gtcatgttgg agttattgag tttcgaccag gccagctcgc catcttcaat aatgacaact
     3121 ggcttgcctt ccagaagatc ttccagtttt tcactgtgcg ccatcaacca cattaccagg
     3181 cggtataaca acgccagagt aataaagacg ataagtaccg ggaccatcgg cacatcatca
     3241 taaaacgcca catctcccgc cgctgatccc agcgtcagaa tgattaaaac ttcaaacagc
     3301 gacatctgcc gcacaccgcg tcttccggtc attttgagga acaaaaagac cagtacaaag
     3361 gtataaagac tacgtagtgc aacttcgcca aggaaatcaa aaggcacttt atcaaatgcc
     3421 atacggtgga gatcgaacgc cttcattttt atgctcttaa cgtggtttat ccgctaaaag
     3481 catagtcaac agttcatcgg catggcggcg gtgatagcgc ctttgagata aaacccgtat
     3541 tattgcgcgc tttccgtacg actaaagtga ttttcatgac gcaaacattt atccccggca
     3601 aagatgccgc tctggaagat tccatcgctc gcttccagca aaaactttca gacctcggct
     3661 ttcagattga agaggcctcc tggctgaatc ccgtgcctaa cgtctggtct gtacatattc
     3721 gcgacaaaga gtgcgcactg tgttttacca acggtaaagg cgcaaccaag aaagcggcgc
     3781 tggcttctgc actcggtgaa tatttcgagc gtctctcaac caactacttt tttgcggact
     3841 tctggctggg cgaaaccatc gccaacggtc cgttcgtgca ttatcccaac gaaaaatggt
     3901 tcccactgac cgaaaatgac gatgtgccag aagggctgct cgatgaccgt ctgcgcgcgt
     3961 tttatgatcc ggagaatgaa ctgaccggca gcatgctgat tgacctacaa tccggtaacg
     4021 aagatcgtgg tatttgcggc ctgccgttta cgcgtcagtc cgacaatcag accgtttata
     4081 ttccgatgaa tatcattggt aacctgtacg tctccaacgg tatgtccgca ggtaataccc
     4141 gcaacgaagc acgcgttcag ggattgtctg aagttttcga acgctacgtg aaaaaccgca
     4201 ttattgctga aagcatcagc ctgccagaga tcccggcaga cgtgctggcg cgttacccag
     4261 cagtagttga agccatcgaa acgctggaag cggagggttt cccaatcttc gcttatgacg
     4321 gttcgcttgg cggccagtat ccggtgattt gcgtggtact gttcaatcct gctaacggta
     4381 cctgctttgc ctctttcggt gcgcatcctg attttggcgt agcactggaa cgtaccgtga
     4441 ccgagctgct gcaaggtcgt ggcctgaaag atttggatgt gtttactccg ccaaccttcg
     4501 atgatgaaga agtcgctgaa cataccaacc tcgaaacgca ctttatcgat tccagcggtt
     4561 taatctcctg ggacctgttc aagcaggatg ccgattatcc gtttgtggac tggaatttct
     4621 ccggcaccac ggaagaagag ttcgccacac tgatggctat cttcaacaaa gaagataaag
     4681 aagtttatat tgccgattac gagcatctgg gcgtttatgc ttgccgtatt atcgtgcctg
     4741 gcatgtccga tatttatccg gctgaagatc tgtggctcgc gaataacagt atgggcagcc
     4801 atttacgtga aacgattctt tcgctaccag gcagcgagtg ggaaaaagaa gattacctga
     4861 acctcatcga gcaactggat gaagaaggtt ttgatgactt tacccgcgtg cgtgagctgt
     4921 tgggtctggc gaccgggtcg gataacggtt ggtacaccct gcgtatcggt gaattaaaag
     4981 ccatgctggc gctggctggt ggcgatctgg aacaggctct ggtctggacc gaatggacga
     5041 tggagtttaa ctcatcagta tttagtccgg aacgcgccaa ctattatcgc tgcctgcaaa
     5101 cgttgttatt actggcacag gaagaagatc gccagccgct gcaatatctg aatgcgtttg
     5161 ttcgcatgta cggcgcagat gccgtagaag ccgccagtgc ggcaatgagc ggcgaagcgg
     5221 cgttttacgg cctgcaaccg gtagatagcg atctgcacgc gtttgctgca catcagtcgt
     5281 tgttgaaggc ctacgaaaag ctgcagcgcg ccaaagcagc attctgggca aaataaaatc
     5341 aaatagccta cgcaatgtag gcttaatgat tagtctgagt tatattacgg ggcgtttttt
     5401 taatgccccg c
//
LOCUS       DAPHBJ010000247         5374 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.249, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000247 DAPHBJ010000000
VERSION     DAPHBJ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.249"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            17..568
                     /locus_tag="RHZ76_004176"
     CDS             17..568
                     /locus_tag="RHZ76_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HDV2814699.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQSDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPSKDATPGNVYAQADYKIAYE"
     gene            628..3126
                     /locus_tag="RHZ76_004177"
     CDS             628..3126
                     /locus_tag="RHZ76_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV2814700.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVIIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLSTLINNKRTPIHLDLQWVLIDNLTAVCVTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFSITNLFNTLQGQLDVKVEEE
                     DGRVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLATGVGFNLNSLGSLSFDVTRSDAQLHNQNKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVSMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRINWEDDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNNISHTASWYDSSDRNNSWSVSASGDNDEFKDMEASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDETSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCCIQPPKSSNLTTGTVILPCIS"
     gene            3148..3897
                     /locus_tag="RHZ76_004178"
     CDS             3148..3897
                     /locus_tag="RHZ76_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV2814701.1"
                     /translation="MRYLNTKNIIAAGVLLSCMNSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKKLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPNEQIQIRMITQEKIAQL
                     PKDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMTYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGVTMLPQTQSLMVLPFSHADTQFKNVPSNFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            3899..4963
                     /locus_tag="RHZ76_004179"
     CDS             3899..4963
                     /locus_tag="RHZ76_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2814702.1"
                     /translation="MRYLLTVIAFIMGFSALPVWAMNCYAEHEGGNTVVIGYVPRIAI
                     PSDGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWVNIMMLLASGQDFYNQNSY
                     TLGVTYNGVDYDSTSTRAIAAPECIDVKGAGIYSNTYKNPAVCSGGPEPQLSVTFPVR
                     VQLYIKLAKNANKVNKKLVLPDEYIALEFKGMSGTGTIDTDKNLTFRIRGLNNIHVLD
                     CFVNVDLEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDIGNGLLLKIFHNDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(5006..5206)
                     /gene="glgS"
                     /locus_tag="RHZ76_004180"
     CDS             complement(5006..5206)
                     /gene="glgS"
                     /locus_tag="RHZ76_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HDV2814703.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT         1656 a         1133 c         1096 g         1489 t
ORIGIN      
        1 tatataaagg aatattatgt ctgcttttaa aaagtcactt cttgttgctg gtgtagcaat
       61 gattctttcc aataacgtgt ttgctgatga aggtcacggt attgttaaat ttaaaggtga
      121 agttatctct gctccatgtt ctattaaacc aggtgacgaa gatttaactg taaatctggg
      181 tgaagtagca gataccgtac tgaaaagcga tcagaaatct ttggcggaac ctttcactat
      241 tcacctgcag gactgcatgc tgagtcaggg tggaactact tattcaaaag cgaaggttac
      301 tttcactaca gcaaatacca tgacgggtca gtctgatctt ctgaaaaata ccaaagaaac
      361 tgaaatcggc ggcgccactg gcgttggcgt acgtattctg gacagccaga gcggcgaagt
      421 aactttaggt actccggtag taattacgtt caacaatacc aacagctatc aggaactgaa
      481 tttcaaagct cgtatggaat ccccaagcaa agatgccacc ccgggcaacg tttacgctca
      541 ggctgactac aagattgctt acgagtaatc gcatttggcc gggttcaccc ggccacatca
      601 ttaacggatt aatgataagt ggatcagatg tataaaaaat taaaattaac cacaataagc
      661 gaattgatta aaaatattta ttgttcatta tccgttatca tcattggttg tgcgtcagct
      721 tatgccgttg aattcaacaa agatttaatc gaagccgaag atcgtgaaaa cgttaacctt
      781 tcccaatttg aaactgatgg ccaattaccc gtcggcaaat attcactaag cactctgatt
      841 aataataaga ggacgccaat ccaccttgac ctccaatggg tattaattga taacctaact
      901 gcagtttgcg tgacaccaga gcaattaaca ttattaggct ttactgatga gattattgaa
      961 gaggctcagc aaaacctgat cgatggttgt taccctatcg aaaaagaaaa acaaattaca
     1021 acttatctcg ataaagggaa aatgcaatta tccatatctg cacctcaggc atggttaaaa
     1081 tacaaagatg caaactggac gcctcctgaa ctttgggatc atggtattgc tggggcattt
     1141 cttgactaca atttatatgc ctctcattat gcaccacatc agggcgataa ttcacaaaat
     1201 ataagttcct atgggcaggc tggggttaat cttggggcct ggcgcctgcg tactgattac
     1261 cagtacgatc agtcatttaa caatggcaaa agccaggcga acaacctgga ttttccgcgt
     1321 atttatttgt ttcgcccaat cccagcaatt aatgcaaaac taactatagg tcaatacgat
     1381 actgaatcct ctattttcga ctctttccat ttttctggcg tttcgttgaa aagcgatgag
     1441 aatatgttgc cgcctgatct acgtggttac gcaccgcaaa tcacgggtgt tgcacaaacg
     1501 aatgcaaagg tcactgtctc acagaacaac cgtattattt atcaagaaaa cgttcctcca
     1561 ggcccatttt ctattaccaa tttattcaat acattacagg ggcaactgga cgtcaaggtt
     1621 gaagaagagg acgggcgcgt cacgcaatgg caagttgcat ctaatagcat tccttatctg
     1681 acgcgtaaag ggcagattcg ctacactact gcaatgggga aaccgaccag cgttggcggc
     1741 gattccttac aacagccctt cttctggacc ggtgaattct catggggttg gctgaacaat
     1801 gtatccctgt atggtggttc agtattaaca aaccgtgatt atcaatccct ggctaccgga
     1861 gttggtttta accttaactc gttgggctca ttatcctttg atgtcacgcg atctgatgct
     1921 cagttacata atcagaataa agaaacgggt tatagctacc gtgctaacta ttcaaaacgt
     1981 tttgaatcta ccggtagcca gctcactttc gctggctacc gtttctctga taaaaacttt
     2041 gtgtcgatga atgaatatat caatgacact aaccattaca cgaattatca gaatgaaaaa
     2101 gagagttata ttgtcacgtt taaccagtat cttgaatcat tgagattaaa tacatacgta
     2161 agtctggctc gtaatactta ctgggacgcc agcagtaatg tgaattattc attatcactt
     2221 agccgcgatt ttgatatcgg cccattaaaa aacgtatcca cctcactaac atttagccga
     2281 ataaactggg aagatgacaa ccaggatcaa ctgtacctaa atatttcaat tccctgggga
     2341 accagtagaa cattgagcta tggtatgcaa cgaaatcagg ataacaacat ttcgcatact
     2401 gcttcgtggt atgactcttc cgatcgaaat aattcctgga gcgtttctgc ttcaggcgac
     2461 aatgacgaat ttaaagatat ggaggcgtca ctgcgcgcca gttatcagca taataccgag
     2521 aacggtcgtc tctatctctc cggtacatca cagcgggaca gctactattc tctgaatgcc
     2581 agttggaacg gttcattcac tgcaactcgc cacggcgccg ctttccatga ctatagtggt
     2641 agtgctgact cgcgttttat gatcgacgca gacggcgctg aagatattcc gttgaataat
     2701 aaacgcgcgg taactaatcg gtatggcatc ggagttattc catcagtcag cagttacata
     2761 acaacatcat taagcgttga tacccgaaat cttccagaaa atgtggatat cgaaaactcg
     2821 gttatcacca ccaccttaac cgaaggtgct attggctacg ccaaacttga tacccgcaag
     2881 ggctatcaaa tcatgggggt tattcgcctg gcagatggta gtcacccacc actggggatt
     2941 agcgtaaaag atgaaaccag ccacaaagaa ttaggacttg ttgctgatgg cggctttgta
     3001 tacctcaacg gcattcagga tgacagcaaa cttactttac gctggggtga caaatcttgc
     3061 tgtattcaac cacccaagag cagcaactta accaccggaa cggttatttt accgtgtatt
     3121 agctaaaatt aattcaggat gaaactaatg cgctacttaa atacaaagaa tatcattgca
     3181 gcaggagtat tactctcctg catgaacagc attgcctggg gtgccatcat cccggatcgt
     3241 acccgtataa taatgaatga gtcagataaa ggcgaagcat taaaacttac taatcaaagt
     3301 aaaaaactgc cctatctggc ccaaacatgg atagaagata cgaaaggcaa taaatctcgt
     3361 gactttattg ttacggttcc tcctatggtg cgtttaaatc ccaatgagca aattcagatt
     3421 agaatgataa ctcaggaaaa aattgctcaa cttcctaagg acagagaaac tctgttctat
     3481 tttaacgtgc gagaaatacc gccaaaaaca gataaaaaaa atgtgatgca agtgactatg
     3541 cagcacgctt tgaaattatt ctggcggcca aaagccattg agttagaaga cgatggggta
     3601 atgacttacg aaaaagttga aatcatccgt agaaatgatg gttcgatccg ctttaacaat
     3661 aaaatgcctt atcacgttac gctgggttat atcggaacaa atggggtcac gatgttaccg
     3721 caaacgcaaa gcttaatggt cctccctttt agccatgcag atacgcagtt taaaaatgtt
     3781 ccatcaaatt ttcaggttgg ttatatcaat gattttggtg gcttaagttt ttatgaaata
     3841 aactgcccaa cagtgaataa ttcttgtaat gtttccgtag ccaaacgaga taaataaaat
     3901 gcgctactta ttaactgtaa ttgcatttat tatgggtttt agtgcgttac ctgtatgggc
     3961 gatgaactgc tatgctgaac atgaaggggg gaataccgta gtcataggct acgtaccaag
     4021 aattgctatc cccagcgatg gtaaaaaagg tgataaaatc tggcaaagca gtgaatattt
     4081 tatgaatgtc ttctgtaata atgcactacc cgccccatca ccaggagaag aatacccatc
     4141 tgcatgggta aatataatga tgttgttagc atcaggtcag gacttttata atcaaaactc
     4201 atatactctt ggtgtaacct ataatggagt cgattatgat tcaacctcca cacgagctat
     4261 cgcagcacca gagtgcattg atgttaaagg cgcagggata tacagtaata cctataaaaa
     4321 ccccgctgtc tgtagcggtg gtcctgaacc tcaattgtca gtaacttttc cagtacgtgt
     4381 acagctgtat attaagctgg ctaaaaatgc taataaagta aataaaaaac ttgtattacc
     4441 tgacgaatat atcgccctgg agtttaaagg tatgagtggc acaggaacta tagacacaga
     4501 caaaaatttg accttcagaa ttcgtgggtt aaataatatt catgtccttg actgctttgt
     4561 taatgttgat ctggaaccag ctgatggcgt tgtcgacttt ggtaaaataa attcccgaac
     4621 aattaaaaat accagcgtga gtgagacgtt tagcgtagtc atgaccaaag atccgggtgc
     4681 ggcctgtact gagcagttta atattttagg gagttttttc actacggata ttttgagtga
     4741 ttatagccat ctggatatag gtaatggtct gctattgaag atatttcata acgatggaac
     4801 agcaacggaa tttaaccgct tctcacaatt tgcttctttt tcatcgtcta gtgcgccttc
     4861 ggtcaccgca ccattcaggg cagaactgag tgcgaacccg gcagaaacgg ttgttgaggg
     4921 accgtttagt aaagacgtaa tcctgaaaat cacctataac tagtatctaa tacaaacact
     4981 aaaacgggcc atcaggcccg ttatatcagt gctctaactc cagctctctt gcctgcatca
     5041 tctgttgaca ataccaggca taacgtgcgc aaaaccaggt tctatgttct tcatccatgt
     5101 taccagtaac ggccagaata tcgaccgggc gaccttctgc gtgcattctg gcaaaactac
     5161 gcgccaggaa atcgaaatta tttaaagaat taagactatg atccataagc acatcctccg
     5221 gttatcgcat accttttaag gccatatgct ttttctgatt tcagtattcc ccaatcagtg
     5281 tagtgccttt aaacataacg tgcgtaaata tattgtcccc gatcacactt tttagtgaaa
     5341 aataaaaaac catattaaaa gactttctct tcca
//
LOCUS       DAPHBJ010000248         5373 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.250, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000248 DAPHBJ010000000
VERSION     DAPHBJ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.250"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            110..544
                     /locus_tag="RHZ76_004181"
     CDS             110..544
                     /locus_tag="RHZ76_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HDV2814704.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            899..1279
                     /locus_tag="RHZ76_004182"
     CDS             899..1279
                     /locus_tag="RHZ76_004182"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDV2814705.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDATAEQPESLAEAPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            1276..1581
                     /gene="tnpB"
                     /locus_tag="RHZ76_004183"
     CDS             1276..1581
                     /gene="tnpB"
                     /locus_tag="RHZ76_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV2814706.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGID"
     gene            1673..3063
                     /locus_tag="RHZ76_004184"
                     /pseudo
     CDS             1673..3063
                     /locus_tag="RHZ76_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(3302..4660)
                     /locus_tag="RHZ76_004185"
     CDS             complement(3302..4660)
                     /locus_tag="RHZ76_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HDV2814707.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            4942..5073
                     /locus_tag="RHZ76_004186"
                     /pseudo
     CDS             4942..5073
                     /locus_tag="RHZ76_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1254 a         1411 c         1468 g         1240 t
ORIGIN      
        1 aagccagctg gcactgcaga caatgtcacc acgattatta tggggagcag tactgcacac
       61 actgccagac cggaggattc agtattcccc ggacaactca ggaggaaata tgcgaattct
      121 gaactgttat atggcgaatg acagcaaagg ccattttgtt acggcgaaag aagctgcgaa
      181 gcacaaccga caggacgttt tgtgctgtgt gtcctgtgga tgcccgttaa cacttcagcg
      241 gggcaatgac ggacaaccac cgtggtttga acatgaccag atgactgtcg ctgaaaaaat
      301 cctgctgcga tgtacctggc ttgacccggc agagaaagag gcccgtcgtt tgcatctgca
      361 gggcatgacg gttccggatt atacggtgaa ggtgagaaag tggttttgtg tgatgtgtga
      421 cgaagattat gagggggaaa agtgctgccc acgctgcggt accggggtat acagcagggc
      481 gtgggggcgg caggaggtgc cgtcggaaga tgccagggct gataatccgt tacagaggct
      541 gtaatggttg cctccggagc agtttgcggt gatgcctttc ccgggaatgg attgccggtg
      601 cggggactgt ggagcttaat tccggtgttg gtggcctttc cgttgatttt atgccaacag
      661 ccccctgtga ctgacaggct gcctcgtcat tccattcgta tctccagtaa cagggggttg
      721 tattgtattt tattgtgccg gttcaggtgt gcgtttcccg gcgcgtctgc accggcttaa
      781 ccaaattcaa cagggataaa atagtggtaa gcgtacagcc tgaaccgtct ggtcagaatc
      841 tgacgaatta gacaaagtgg tgtccaccaa ataagtagtg ggaaccaaag tgtcagatat
      901 gcagaaaaat gtgactcccg gcaggcgaaa aggctgccct aattatcctc ccgaatttaa
      961 acagctgctt gttgctgcct cctgtgaacc cgggatatcc atctcaaaac ttgctcttga
     1021 aaatggcatt aacgccaatc tgttgttcaa atggcgacaa caatggcgcg agggaaagct
     1081 gctattacct tcttcagaga gcccccagct acttcctgtg actctcgatg caactgccga
     1141 acagccagaa tcgctcgcag aggccccgga aaccctcagt atcagctgtg aggtaacgtt
     1201 ccggcacggg acgctccgct tcaatggcaa tgtcagcgaa aagctcctga ctctgctgat
     1261 acaggaactg aagcgatgat cccgttacct tccgggacca aaatttggct ggttgccggt
     1321 atcaccgata tgagaaatag cttcaacggc ctggctgcga aagtacaaac ggcgctgaaa
     1381 gacgatccca tgtccggcca tattttcatt ttccggggcc gcagcggcag tcaggttaaa
     1441 ctgctgtggt ccaccggtga cggactgtgc ctcctgacca aacggctgga gcgcgggcgc
     1501 ttcgcctggc cgtcagcccg tgatggcaaa gtgttcctta cgcaggcgca gctggcgatg
     1561 ctgctggaag gtatcgactg acgacagccc aagcggctgc tgacctccct gaccatgctg
     1621 taaatctctt tatcctggtt gtcacagaat aagcccggta aaatacgggc ttatgaacga
     1681 catctcttct gacgacatct tcctgctgaa acagcgcctg gccgaacagg aagcgctgat
     1741 ccacgccctg caggaaaagc tgagcaaccg ggagcgcgaa atagaccatc tgcaggcgca
     1801 gctggataaa ctccgccgga tgaacttcgg cagtcgttcc gaaaaagcct cccgccgtat
     1861 cgcacaaatg gaagccgatc tgaaccggct tcagaaagag agcgatacgc tgactggtag
     1921 ggtgtatgac ccggcagtac agcgtccgtt gcgtcagacc cgcacccgta agccgttccc
     1981 tgaatcacta ccccgtgacg aaaagcgact gttgcctgcg gcgccgtgct gcccgaactg
     2041 cggcggttca ctgagctatc tgggcgagga taccgccgaa cagctggagt tgatgcgtag
     2101 cgccttccgg gttatccgga cggtacggga aaaacatgcc tgtactcagt gcgatgccat
     2161 cgtgcaggca cctgcacctt cgcggcccat cgagcggggt atcgccggac cggggctgct
     2221 ggcccgcgtg ctgacctcga agtatgcaga gcacaccccg ctgtattgcc agtcagaaat
     2281 atacggccgg caaggtgtgg agctgagccg ttcactgctg tcgggctggg tggatgcatg
     2341 ctgccggctg ctgtctccgc tggaagaggc gcttcatggc tatgtcatga ctgacggcaa
     2401 actccatgcc gatgataccc cggtccaggt actgctgccg ggtaataaga agacgaagac
     2461 cgggcggttg tgggcgtatg ttcgtgatga ccgcaatgcc gggtcagcgt tggcacctgc
     2521 agtgtggttc gcttacagcc cggacagaaa aggcatccat ccgcagactc atcttgcttg
     2581 cttcagcggt gtgctgcaag cggatgcgta cgccgggttc aacgagctgt atcgcaatgg
     2641 tgggataacg gaagctgcct gctgggctca tgcccgccga aagatccacg atgtgcacgt
     2701 ccgcatcccg tcagcactga cggaagaagc cctggagcag atcggtcagt tgtacgccat
     2761 agaggcggat ataaggggaa tgccggcaga gcagcggctt gctgaacgtc agcgaaaaac
     2821 gaaaccgctg ttgaaatccc tggaaagctg gttgcgtgaa aagatgaaga ccctgttctt
     2881 cggctctggc catggtggtg agcggggagc gctactgtac agcctgatcg ggacgtgcaa
     2941 actgaatgac gtggatccag aaaggaaagc taccttcgcc atgtgcttgg cgtcatagca
     3001 gactggccgg tcaaccgggt cagcgaactg cttccgtggc gcatagcact gccagctgaa
     3061 taacacatcc ccgtcaatac ggccctcgct gtacgcttac ttttgttcgg actgcttatg
     3121 gcctttttcc actggtggcg caggaaagtt aaccggctgc caccatggga tgacgtgtaa
     3181 accgtgcagg catgttttca cgtggtgacg ctttcattgg gggtctacgc cggaacccca
     3241 atattgttca catacatgaa tttgaaaaac taagcgatac tgctgtccgt accttataaa
     3301 cttatttcaa cgcttccggc agcgtcacaa tccacagttc actgtcagac tccggtttct
     3361 ccagtggctt cactctgagg gtcgtttcaa cctgcttgtc gtcaagcgtc agctttcctt
     3421 ccgcgttcac ccgataatcc ttgaatgtct tgccctcgac cggatgttgc atcgccacct
     3481 tcacgccaaa gtcgttatcg ttcgccaccg ccagcgtttt gctgtcgatc agcgccaacc
     3541 cttcagcctt ctcctgctgc cagcccaagg agcgcagatc aacaacctgc gttttctgcg
     3601 caagggtaat gccgcgctgt gacagggttt tctcatcatc aaactccgga tactcacccg
     3661 gcttgtcgaa gcctgacagg tcgctcgcct tattgagatc gacctcgtaa atcaggttac
     3721 gcattctgtt gtttttatct cttccctgtt cgatcagcag gatgtgctgg ttatcgagcg
     3781 ccacgatgtc tccgatttta gcgtcgctgt ttttgctgta ggccgcgctg tcgataggat
     3841 aaccgtacat cgcggttttt ccgcttgccg gatcgaagct caccagacgc gtaaacagcg
     3901 cctttttctt gctcttagcg tcgatatcca gcgtactttg cacggcgaca ataatacgcc
     3961 cgtccggcat acgggtgagg ccttcaaagc cgcggttcgc ctgacgccat ttgaggatat
     4021 ttggcaaacc gcccgcaatc gctttttccc cttccgccgc ctgcggaccg tggattgcca
     4081 ggattttccc tttactgtcg atgttaatca ggaacgggcc atactcatcg cacagccagt
     4141 aacctccttt accatccgga gtgatccctt ccgtatccag cccgcgatta tcccccttca
     4201 gtctatgtaa cgtgtcgctg aaggccactt cattggtgga gccaatcacg tcgcttgcca
     4261 gcggcagacc gttgatcgcg cctttatcgt catgcagagg acggggatcg atggcctccg
     4321 ctttgccgtt ttgtacacga atcgtcatca gcagcggcgc gaaatctggc gtaacgaaaa
     4381 ttttagtttc gtttttcccc tctttcggtg aatccgcgtt cggaccgcga tcggtaatgg
     4441 ttgcgaatgt cagggcatcg ccctgtttac cggtgaacag caggccagaa cctatcccaa
     4501 ccggaaggcc gttcgggaat gcactggcaa atgcgccggc atagttcacg tgcgtgcctt
     4561 ctggaaaact gacgacatag cgctcagccg taggttgtgc cgccagggca gagaaagaga
     4621 gcgtacaacc gattagcacg ggaataattt ttcttttcat attgttagca tccataaagt
     4681 gaagatcaca ctacaccata aagaagaaac gagtcaggaa aatgacatcg aatggcaaca
     4741 tgcgcgggcc tgaggccaaa gaaatgtaaa acactgttgt tatttgtaac aacggaaaca
     4801 tttacggaaa ttatttgtct ttcttcgtga tcaaaaatgc tgtgtcttgt tggattggag
     4861 tgatgagggc tgcgtttagg aaacgcccta tattgtacgg ttgcattatc gcggtagggg
     4921 taatgcacgt gtcagtatga tgagctgacc acactcacgt gggtggactg gtataacaat
     4981 cgacgattgc tggaaaggca gggccatatc ccaccggcag aagcagaaaa agcttattat
     5041 gcttccatcg gaaacgatga tctggcagcc tgagttcaca gataaaacac tctccaggaa
     5101 acccggggcg gttcaattgc tggataagat cataacgata caccgggtta aacaggtggt
     5161 cactgcttca ctgaagctgc gtgattatga tggtcaggta gcagaagcta tgcccatgat
     5221 acaaaaatga cgaaagccga aatgtctgaa agcgtgtgta ttacctgaaa atatgaccgg
     5281 ctccggggta cgtaaccgat tcaacaaagc caatcgatat tacaaaacga gaggaactat
     5341 atatttcgtt ttcagataag actccttgag aga
//
LOCUS       DAPHBJ010000249         5227 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.251, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000249 DAPHBJ010000000
VERSION     DAPHBJ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.251"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(212..1465)
                     /locus_tag="RHZ76_004187"
     CDS             complement(212..1465)
                     /locus_tag="RHZ76_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDV2814708.1"
                     /translation="MPDKREQMTYASQAVKRTPHEVTDHFIKMVHARIAEVSGWRYVF
                     DRIPAFKDACDKAPGQVPCPFSGVGKSKFRFRKKDLFTGCAIHNDFPVNAFCDGIDVL
                     AEYYKLSKTQTCKKILTDFFGMDLYAPLTDADLESERRYKSTVRATETLDSDEVEKRG
                     RKLEVIYHYTGEIKPESPVWVYLRNRGLNRVLSNLPKDLGLNKRLYYMDKSLEKPTIY
                     PGMIAIYRDTRGRPLTIHRTFVELNGDKAHVENPKLMMKPPADMTGGSIQLYDPHFNP
                     GTRTWTLGVAEGIENALSVTEATSTPCWAASSAWCLENVEVPDSLLPPPGVKVIQFYI
                     WADKDLVNTKGTSPGMESAKRLQERMKEFFAKRYPTSELIIKVFEPDFDIPVGKKGVD
                     WNDVLKLTGPDGFPVKWAPECLAQL"
     gene            complement(1559..1780)
                     /locus_tag="RHZ76_004188"
     CDS             complement(1559..1780)
                     /locus_tag="RHZ76_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814709.1"
                     /translation="MTQIKTYRVEYEKVGTMHRVRIFGRMGEIVKSELPEERILRDVS
                     IPEGNGEMATSMVDGFIQRLENIGFKTEA"
     gene            complement(1852..3864)
                     /locus_tag="RHZ76_004189"
     CDS             complement(1852..3864)
                     /locus_tag="RHZ76_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDV2814710.1"
                     /translation="MKFDLKSSPRHIQRLTNIASVISGINGIYVIIDNKVSTPYFNTQ
                     NNVCVLPNGDYSDERFVKLIEGFICHEAGHGRYTEHEVYREAFVGELINADGFISIDD
                     DLKADFQNLKQKQKAYARACRLKGLINLFDDVQMEEKTGIDYQEAKKRLAVTYALMVE
                     AGRMTVDISSTPQNPVQFIEMYLLNTLRVNVLQQEGHKETLDPFFDYAKKILAPVTSE
                     VDEIIHQALSCKSTQNCDSLARKTLALLERLRDEAKEKQQEEEQSKDPHDDTDESSGS
                     ENEPDTEPNGDSQGEGDGDKEGQGDDSGDGKAPAEGNGLPDQSGGDEQNAENSNGEPD
                     GESEGDESATPGSDDADSTPSLNDSSNGNSSDGESNFSPEQWDMLAKLLDDFLNSDEE
                     SEDFHEVLAKEISVIAASVSDEVKAEFGASEWDVPDLNIDLNVYNEALNISQTLGADL
                     SVLQQVKMRGQFKTRDRGLSFDINRLIQSPMGVRDVFRSQSESKNRGHVGLVIVRDIS
                     GSMSLEHRYIHAIKTDLALTLAIEAISKMHVANVIYPFVDKDFEVIKTFDENAEEKLS
                     KFSLGCKGNNTPTGSALNAALELLLESQFDRKIVFLITDGYPTESAYTINDVFSVAES
                     NGIEIAGVGIKTDVLMGFNEGTFVNVDDISLLPNEVSKLVHQILSN"
     gene            complement(3942..5069)
                     /locus_tag="RHZ76_004190"
     CDS             complement(3942..5069)
                     /locus_tag="RHZ76_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3150 domain-containing protein"
                     /protein_id="HDV2814711.1"
                     /translation="MTFNTSKLNKVSAKEISENFIKAYPDLLEGAVITKIEISGCQGS
                     RRTDKIELSYGGDDITDQKSVSKSEVRWIDIKALSFKSTITSRISTLCSRLCIRYSNM
                     WILPVERMEEFLEEAQEIEREFQAGIQNVVDNYDTHIQAEKDRSPLMSDLIDQLKLTK
                     DDFVKSFRFNLAHFIPFSPISVEGDETQDFYQEQLITDLADEAMKVYTKISKNDNLRS
                     STIDRLKQMQDKIISFMFIHKEAVVLAEAIKHIMNNLPKGAISSPRDVAVLKQWFYFM
                     SDASMLKRIISGEQKVTDWLDSITRSFNIDSQVTAANSLNTAEDDVFSSANVFREDPV
                     EDLENKAVTAPEPAVGPDEDVSIQASGSNRAFDCELELTGW"
BASE COUNT         1461 a         1227 c          990 g         1549 t
ORIGIN      
        1 ttaagtttta tggatatacc atgtcataca accgaccgta gccagatgta tcgcacattt
       61 attaaactga gcaagcatct cagccgtaaa aaaaacctga ataacaggtg attttcacga
      121 aggaaatcgg attatctgtt taggatttaa cgtaaataaa cactaaagag ataacccccc
      181 ccggcctgaa aaggcggggg agttatcgga tttaaagctg agcaagacat tcaggagccc
      241 attttacggg gaatccatcc ggtcctgtta attttagtac atcgttccag tcgactcctt
      301 tcttccctac aggaatatcg aaatcaggtt caaagacttt aattatcagc tctgaagtgg
      361 gatatcgctt tgcaaaaaac tctttcatac gttcctgaag tcgtttagct gactccattc
      421 ccggggaagt gccttttgta ttcacaagat ccttatccgc ccagatgtaa aactgtatta
      481 ctttcacacc agggggaggc aggagactat ccggcacttc aacgttttca aggcaccagg
      541 cagagctcgc ggcccagcac ggtgttgaag tcgcttcagt aactgacagc gcgttttcta
      601 tgccttcagc cacacctaac gtccaggtac gggtacctgg attgaagtgt ggatcgtaca
      661 gctgaattga gccgccagtc atatcggcag gcggtttcat catcagtttc ggattctcaa
      721 catgtgcctt atcaccattt aactcgacga acgttctgtg gattgtcaga ggacgcccac
      781 gggtatcgcg ataaattgcg atcattcccg ggtaaatcgt gggtttttcc agtgacttat
      841 ccatgtagta aagtcgtttg ttcagaccta aatccttggg aaggttgctc agaactcgat
      901 taagacctcg atttcggaga tacacccaca cagggctctc aggtttgatt tcaccggtat
      961 agtgatatat cacttcaagt ttacgccctc gtttttcaac ttcatcgctg tcaagcgttt
     1021 cggttgcacg aactgttgat ttataacgac gttcactttc gagatccgca tcagttaatg
     1081 gagcgtacaa atccatccca aagaaatcag tcaggatctt cttacaagtc tgggtcttgc
     1141 tcagcttgta gtactctgcc aatacatcaa taccgtcaca aaatgcattc actggaaaat
     1201 cattgtgaat ggcacaaccg gtaaagaggt cctttttacg gaaccggaac tttgatttcc
     1261 cgaccccact aaatgggcaa ggaacctggc caggtgcttt atcacaagcg tctttaaatg
     1321 ccggtattct atcgaataca tagcgccagc ctgatacttc cgctatacga gcgtgaacca
     1381 tttttataaa gtggtcagtg acttcatgtg gtgtacgctt aacagcctgg gatgcataag
     1441 tcatttgttc tctcttatct ggcattgtgg gtttctcaat gccctctgcg atgcagaaaa
     1501 cagggagaaa cccacaacgc agaatctaag aatgagggcc cacaagggcc ctcaacaatt
     1561 acgcttcagt cttgaagccg atattttcaa gacgctggat aaaaccatca accattgacg
     1621 ttgccatttc tccattaccc tctggtatag atacatcccg gagaattctt tcttctggca
     1681 attcactttt gacaatttca cccatgcgac caaaaatacg gacacgatgc atcgtcccga
     1741 ctttttcata ctcgacacgg taagtcttta tctgagtcat atattgctcc ttaatccttc
     1801 agggagcaaa gcgacccaac cgggcgtcgt tttgccccga ttgggtagag tttaattaga
     1861 tagaatttga tgtactaatt tactgacctc attcggcaat aaagaaatat catcaacatt
     1921 tacgaaggtg ccctcattaa aacccatcaa cacatcagtt ttaataccaa caccagcgat
     1981 ttcaatgccg ttactttcag caactgaaaa aacatcatta atggtgtaag ccgactcagt
     2041 cggataacca tcagtaataa ggaaaacaat tttcctgtca aactggcttt caagtaacaa
     2101 ctctaaagca gcatttaatg cagagcccgt tggggtatta ttgcccttgc agccaagtga
     2161 gaattttgaa agtttctctt ccgcgttttc atcaaacgtt ttgataactt caaaatcttt
     2221 gtctacaaac ggataaatca cattagcaac atgcattttt gaaatagctt caattgccag
     2281 agttaatgca aggtcagtct taattgcatg gatataacgg tgttccagac tcattgaacc
     2341 tgaaatatca cgaacaatga ccaagccgac gtggccccgg tttttagatt cgctttgtga
     2401 ccgaaataca tccctaacac ccatcggaga ctgaatcagt cgattaatat cgaatgacaa
     2461 gcctctatca cgggttttaa attgaccacg catttttacc tgctgtaaaa cagataaatc
     2521 tgccccaagg gtctggctga tatttaatgc ttcattgtag acattcagat caatgttcaa
     2581 atctggaaca tcccactcac ttgcaccaaa ttcagcttta acctcatccg acacactggc
     2641 agcaattacg gagatctctt ttgcaagaac ctcatgaaaa tcctcgcttt cctcatcact
     2701 gtttaaaaaa tcatccagca atttggcaag catatcccac tgttcaggag aaaagttact
     2761 ttccccatca gaagaatttc catttgaact gtcgtttagg gaaggtgtcg agtcagcatc
     2821 atccgagccg ggggtcgccg attcatcacc ttcagactca ccatcaggtt cgccgttaga
     2881 gttctcagca ttttgttcat cccctcctga ttggtccggt aacccattac cttccgcagg
     2941 cgccttacca tcaccagaat catcgccttg accttcttta tcaccatccc cttcaccctg
     3001 gctatcacca ttgggttcgg tatcaggttc attctcagag ccagatgatt catctgtatc
     3061 gtcatgcggg tctttcgact gctcctcttc ttgctgtttc tcttttgctt catcccttaa
     3121 acgctctaaa agagccagag ttttacgcgc cagagaatcg caattttgcg tacttttgca
     3181 acttaaagct tgatgaatga tctcatcgac ttctgaagtt acaggtgcaa ggattttctt
     3241 tgcataatcg aaaaatgggt caagcgtttc tttatgccct tcttgttgga gaacgttaac
     3301 ccgcaatgta ttaagcaggt acatttcaat aaactgaacc gggttttgag gggttgaaga
     3361 aatatcaacc gtcattcgcc cagcttcaac catcaaggca taagtcaccg cgaggcgttt
     3421 ttttgcctcc tgataatcaa tacccgtctt ctcttccatc tggacatcat caaaaagatt
     3481 aataagtcct tttagtcgac aagcacgagc atatgctttt tgcttttgtt ttaggttttg
     3541 aaaatcagcc ttcaagtcat catcaatact gatgaagcca tctgcattga tcaattctcc
     3601 aacaaaagct tcacggtata cttcatgctc tgtataacga ccgtgtcctg cttcatgaca
     3661 tatgaatcct tcaattagct tgacaaatct ctcatcgcta taatcaccgt taggtaaaac
     3721 acagacattg ttttgggtgt tgaaataagg tgtactcacc ttattgtcaa taattacata
     3781 aatcccattt ataccgctta tcacgctggc tatattcgtc aggcgttgaa tatggcgtgg
     3841 tgaagatttt aaatcaaatt tcatttgagt acctttattt gataaagggt actcacccag
     3901 atggtgaagt acccatctgg ggatattttt acaagactaa attaccaacc ggtcaattcg
     3961 agttcgcagt caaatgctct attactacca ctggcctgaa tagaaacatc ttcatcaggg
     4021 ccaacagcag gctcaggtgc agtcactgcc ttgttctcaa gatcttcaac cgggtcttct
     4081 ctgaaaacat ttgctgaaga aaaaacatca tcctcagcag tattcagaga gttagccgct
     4141 gtaacctgtg aatcgatatt gaatgaccgg gttatggaat ccagccagtc ggttaccttt
     4201 tgttcaccgg agataatacg tttcagcatt gacgcatcac tcatgaaata gaaccactgc
     4261 tttaataccg caacatcacg gggagaagaa atggcacctt ttggtaaatt attcatgata
     4321 tgcttaatag cttctgcaag aacaacagcc tctttatgaa tgaacatgaa gctaattatt
     4381 ttatcctgca tctgctttaa tcggtcaatc gtgctggaac gcaaattatc atttttgctg
     4441 atttttgtat aaaccttcat agcttcatcg gccaagtcag ttatcagttg ttcttgataa
     4501 aagtcctgcg tttcatcccc ctccacacta atcggtgaaa acggaatgaa atgtgccaga
     4561 ttaaaccgga atgatttaac aaaatcatct ttagtcaatt tcaactggtc aattaaatct
     4621 gacatcaacg gactgcgatc tttctcagcc tgaatatgag tgtcatagtt atcaaccacg
     4681 ttctggatac cggcttgaaa ttcacgttcg atttcttgtg cctcttcgag aaactcctcc
     4741 atcctttcaa ctggcagtat ccacatattg ctatagcgga tgcacaaccg tgaacaaagg
     4801 gttgaaattc gactagtgat agtgcttttg aaactcagtg cctttatgtc tatccagcga
     4861 acttcgcttt tagacacgct cttctggtct gttatatcat caccgccata ggacagttcg
     4921 attttatcag ttcgccgaga gccctgacac ccactaattt caatttttgt tataacagca
     4981 ccttccagca agtcaggata tgcttttata aagttttcac tgatttcctt tgcagaaact
     5041 ttattaagtt tagaagtatt gaaagtcata ggttttctcc agatttagaa atgggaaaac
     5101 ctccccgtcg ggaaagattt cccgataggg ttaataatat tacttttcaa gtgttgttaa
     5161 aagaattcca ggaaattcat tttcgacatc cgccgattga atgctccctt gatacatgaa
     5221 tcctttt
//
LOCUS       DAPHBJ010000250         5166 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.252, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000250 DAPHBJ010000000
VERSION     DAPHBJ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.252"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1294
                     /gene="garP"
                     /locus_tag="RHZ76_004191"
     CDS             <1..1294
                     /gene="garP"
                     /locus_tag="RHZ76_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HDV2814712.1"
                     /translation="TRYLILLIIFIVTAVNYADRATLSIAGTEVAKELQLSAVSMGYI
                     FSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGFVDMFPLAWAGISMF
                     FMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFSLALFSPLLGWLTFA
                     WGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISENGAVVDMDHKKPGS
                     AAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQEKGMSILKVGL
                     VASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLLASTIILCNYTNNTT
                     LVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPLVIGYLV
                     SELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1310..2079
                     /gene="garL"
                     /locus_tag="RHZ76_004192"
                     /pseudo
     CDS             1310..2079
                     /gene="garL"
                     /locus_tag="RHZ76_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            2109..2999
                     /gene="garR"
                     /locus_tag="RHZ76_004193"
     CDS             2109..2999
                     /gene="garR"
                     /locus_tag="RHZ76_004193"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDV2814713.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3096..4241
                     /gene="garK"
                     /locus_tag="RHZ76_004194"
     CDS             3096..4241
                     /gene="garK"
                     /locus_tag="RHZ76_004194"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HDV2814714.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4274..4650
                     /gene="rnpB"
                     /locus_tag="RHZ76_004195"
     ncRNA           4274..4650
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="RHZ76_004195"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1203 a         1240 c         1486 g         1237 t
ORIGIN      
        1 tacccgctat ttaatattac tgattatttt tattgttacc gccgttaact acgccgatcg
       61 tgcaacgctg tctattgctg gtaccgaagt ggcaaaagag ttgcagttaa gtgcggtttc
      121 gatgggttac atcttctccg cttttggctg ggcctacttg ctgatgcaaa tccccggcgg
      181 ctggctgctt gataagtttg gctcgaaaaa agtttacacc tacagcctct ttttctggtc
      241 gctattcacc ttcctgcaag gctttgttga tatgttcccg ctggcctggg cagggatctc
      301 catgttcttt atgcgcttta tgctcggctt ctcggaagcg ccatcattcc cggcgaacgc
      361 ccgaattgtc gccgcctggt tcccgacgaa agaacgtggt actgcctccg ccatctttaa
      421 ctcggcgcaa tatttctcgc tggcgctctt ttcgccgctg cttggctggc tgactttcgc
      481 ctggggctgg gagcacgtct ttaccgttat gggggtgatt ggttttgtgc tgacggcgct
      541 gtggatcaag ttgattcata acccgacaga tcacccacgt atgtctgcgg aagagctgaa
      601 gtttatctct gaaaatggcg cggtggtcga tatggaccac aaaaagccgg gcagtgcggc
      661 agcaagcgga cccaaactgc attacatcaa gcaattgctc tctaaccgca tgatgctggg
      721 cgtatttttc ggacaatatt ttatcaacac catcacctgg ttcttcctca cctggttccc
      781 gatttatctg gtgcaggaaa aaggcatgtc gattctgaaa gtgggtctgg tcgcctcgat
      841 tccagcactg tgtggttttg cgggcggcgt gctgggaggt gtcttctcgg attatctgat
      901 caaacgcggt ttatccctga ccctggcacg taagctaccg attgtgctgg gaatgttgct
      961 ggcttccacc atcatcttat gtaactacac caacaacacc acgctggtgg tcatgctgat
     1021 ggcgctggct ttctttggca aaggatttgg tgcgctgggc tggccggtga tttctgacac
     1081 cgcgccgaaa gagattgttg gcctctgcgg cggcgtcttt aacgtctttg gcaatgttgc
     1141 ctccattgtc actccactgg tgattggcta cctggtaagt gaactgcact ccttcaatgc
     1201 agcactggtt ttcgtgggat gttcagcgct gatggcgatg gtctgctacc tcttcgtagt
     1261 tggcgacatt aaacgtatgg aattgcagaa ataagcaaag gtataagcga tgaataacga
     1321 tgttttcccg aataaattca aagccgcact ggctgcgaaa caggtacaaa ttggttgctg
     1381 gtcagcactc tctaacccga ttagcactga agttcttggt ttggctgggt ttgactggct
     1441 ggtgctggat ggcgaacatg cgccaaacga tatctccacg tttattccgc agttaatggc
     1501 cttgaaaggc agcgccagcg cgccagtagt gcgagtgccg accaacgagc cggtaattat
     1561 taagcgtctt ctggatatcg gtttctataa cttcctgatt ccttttgtag aaacaaaaga
     1621 ggaagcagag ctggcggtgg catcaacccg ttacccaccg gaaggcattc gcggcgtctc
     1681 cgtttctcac cgcgccaata tgtttggcac cgtggcggat tatttcgctc agtcgaacaa
     1741 gaacatcact attctggtcc agatagaaag tcagcagggc gtagataacg tcgatgccat
     1801 tgccgctacc gaagcgtaga cggcatcttc gtcggcccca gcgatctggc cgcggcatta
     1861 ggccatctcg gcaatgcatc acacccggat gtacaaaaag caattcagca catttttaac
     1921 cgtgccagcg cgcacggcaa acccagcggt atcctcgcgc cggtcgaagc cgatgcgcgt
     1981 cgttatctgg aatggggcgc gacgtttgtg gctgtcggca gcgatctcgg cgtcttccgc
     2041 tctgccactc agaaactggc tgataccttt aaaaaataac caccaccgca acaagagaga
     2101 tgattgatat gactatgaaa gttggtttta ttggcctggg gattatgggt aaaccaatga
     2161 gtaaaaacct tctgaaagca ggttactcgc tggtggttgc tgaccgtaac ccagaagcta
     2221 ttgctgacgt gattgctgca ggtgcagaaa cagcgtctac ggctaaagcg atcgctgaac
     2281 agtgcgacgt catcataacc atgctgccaa actcccctca tgtgaaagag gtggcgctgg
     2341 gtgagaatgg cattattgaa ggcgcgaagc caggtacggt attgatcgat atgagttcta
     2401 tcgcaccgct ggcaagccgt gaaatcagcg aagcgctgaa agcgaaaggc attgatatgc
     2461 tggatgctcc ggtgagcggc ggtgaaccga aagccatcga cggtacgctg tcagtgatgg
     2521 tgggcggcga caaggctatt ttcgacaaat actatgattt gatgaaagcg atggcgggtt
     2581 ccgtggtgca taccggggaa atcggtgcag gtaacgtcac caaactggca aatcaggtca
     2641 ttgtggcgct gaatattgcc gcgatgtcag aagcgttaac gctggcaact aaagcgggcg
     2701 ttaacccgga cctggtttat caggcaattc gcggtggact ggcgggcagt accgtgctgg
     2761 atgccaaagc gccgatggtg atggaccgca acttcaagcc gggcttccgt attgatctgc
     2821 atattaagga tctggcgaat gcgctggata cttctcacgg cgtcggcgca caactgccgc
     2881 tcacagctgc ggttatggag atgatgcagg cactgcgagc agatggttta ggaacggcgg
     2941 atcatagcgc cctggcgtgc tactacgaaa aactggcgaa agtcgaagtt actcgttaat
     3001 gacgtcgtgc ccggatggcg tattgcaatc cgggcctgga atccaggccg aataagagaa
     3061 acgccctccg gcgtcatgtg gtaacaggca taggtatgaa aatcgtaatc gccccagact
     3121 cttataaaga aagtttatct gccagcgagg ttgcgcaggc gatagaaaaa ggatttcggg
     3181 aaatttttcc tgatgcacag tacgtttctg ttccggttgc cgacggtggc gaaggaacgg
     3241 tggaagcgat gattgcagcc acccaggggg ctgaacgtca cgcctgggtt acagggccgc
     3301 tgggcgagaa agtgaatgcc agttggggga tctccggcga tggcaaaacc gcgtttattg
     3361 aaatggcggc ggccagtggg ctggagctgg tacctgcgga aaaacgcgat ccactcgtga
     3421 ccacttcacg cggcacaggc gagttaatcc tgcaggcgct ggagagcggt gcgacaaaca
     3481 ttattatcgg cattggcggc agcgctacaa atgatggcgg cgcaggcatg gtacaggcgc
     3541 tgggggcgaa attatgcgac gccaacggca atgaaattgg ttttggcggc ggtagtctta
     3601 atactctgaa tgatattgat atttccggcc tcgatccgcg cttaaaagat tgcgtcattc
     3661 gcgtcgcttg tgatgtcacc aatccgctgg tgggcgataa cggcgcatcg cgcatctttg
     3721 gcccacaaaa gggagccagt gaagcgatga ttgttgagct ggacaataac ctctctcact
     3781 atgccgaggt cattaaaaaa gcgctgcatg ttgatgtgaa agatgtcccc ggtgcaggag
     3841 ctgcgggtgg tatgggcgcg gcgctaatgg cgtttcttgg tgcggaactg aaaagtggta
     3901 ttgaaatcgt cactacggcg ctgaatctgg aggaacatat tcacgattgt acgctggtga
     3961 tcaccggtga agggcgtatt gacagccaga gtattcacgg gaaggtaccg attggtgtcg
     4021 caaacgtggc gaagaagtac cataaaccgg tgattggcat tgcgggtagc ctgaccgatg
     4081 atgttggcgt tgtacatcag catggcattg atgcggtctt cagcgtattg accagcatag
     4141 gtacgttgga cgaagcattc cgcggggctt atgacaatat ctgccgtgct tcacgtaata
     4201 tcgccgcgac actggcgatt ggaatgcgca acgcggggtg acaagggcgc gcaaaccctc
     4261 tatactgcgc gccgaagctg accagacagt cgccgcttcg tcgtcgtcct cttcggggga
     4321 gacgggcgga ggggaggaaa gtccgggctc catagggcag ggtgccaggt aacgcctggg
     4381 ggggaaaccc acgaccagtg caacagagag caaaccgccg atggcccgcg caagcgggat
     4441 caggtaaggg tgaaagggtg cggtaagagc gcaccgcgcg gctggtaaca gtccgtggca
     4501 cggtaaactc cacccggagc aaggccaaat aggggttcat aaggtacggc ccgtactgaa
     4561 cccgggtagg ctgcttgagc cagtgagcga ttgctggcct agatgaatga ctgtccacga
     4621 cagaacccgg cttatcggtc agtttcacct gatttacgta aaaacccgct tcggcgggtt
     4681 tttgcttttg gaggggcaga aagatgaatg actgtccacg acactatacc caaaagaaag
     4741 cggcttatcg gtcagtttca cctgatttaa cgaaaacccg ccagcgtaaa ccggcgggtt
     4801 tttgctttgg gaggggcaga aagatgaatg actgtccacg acgctatacc caaaagaaag
     4861 cggcttatcg gtcagtttca cctgatgtac gtaataaacc gttccggcgg gtttcagatt
     4921 gttgagtgcg ctttattcat gccggatgcg gcgtgaacgc cttatcctgc ctacaaaagc
     4981 acgcaaattc aatatattgc agagatggcg taggcctgat aagcgtagcg catcaggcat
     5041 ttttgcggtt gccatcagtt tcaaacccgc atcgataagt ttttgctttt ggtgcacagg
     5101 gtaagaagca tgaatattta cggtattaat aatagacttt gtaagaagtt attaagtcta
     5161 tattgc
//
LOCUS       DAPHBJ010000251         5062 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.253, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000251 DAPHBJ010000000
VERSION     DAPHBJ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.253"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            43..384
                     /locus_tag="RHZ76_004196"
     CDS             43..384
                     /locus_tag="RHZ76_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdK family transcriptional regulator"
                     /protein_id="HDV2814715.1"
                     /translation="MAQKNRISETEWKQLLPQMASFAHITTDIGYSVLVKGEKSSDVA
                     TRVGRSKQNISSTVKRIWDLYQNTTLKAENGEPLKLVQVWIPASLAETVLKEAAKYSI
                     NNITTSEMEKK"
     gene            complement(399..1190)
                     /locus_tag="RHZ76_004197"
     CDS             complement(399..1190)
                     /locus_tag="RHZ76_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HDV2814716.1"
                     /translation="MNLPTPETYDELQRAYDFFNEKLFSNELPPCLITLQREKRTYGY
                     CSFKRFVGRESGYTVDEIAMNPVYFSIRTIKATLSTLVHEMVHQWQFHFGEPGRRGYH
                     NKQWAARMERVGLMPSDTGEPGGRKVGQSMTHYIIAGGPFDMACDELLTGHFRLSWMD
                     RFPPYQPKPGAVLSPTGKGYIDDEEDDSEHEQEVEEGRDPVELDDEIIEAMRFVTPPP
                     EAPVNKTNREKYSCPVCHINLWGKPGIVVYCGGEHCNKAALVVLK"
     gene            complement(1341..2606)
                     /gene="mucB"
                     /locus_tag="RHZ76_004198"
     CDS             complement(1341..2606)
                     /gene="mucB"
                     /locus_tag="RHZ76_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     MucB"
                     /protein_id="HDV2814717.1"
                     /translation="MFALIDVNGMYASCEQAFRPDLANRAVAVLSNNDGNIVARNYLA
                     KKAGLKMGDPYFKVRPIIERHNIAIFSSNYTLYASMSARFAAVVESLASHVEQYSIDE
                     LFVDCKGITAAMSLDAFGRQLREEVRRHTTLVCGVGIARTKTLAKLCNHAAKTWPATG
                     GVVALDDGARLKKLMSILPVAEVWGVGHRTEKALATMGIKTVLDLARADTRLIRKTFG
                     VVLERTVRELRGEACFSLEENPPAKQQIVVSRSFGQRVETLTDMQQAVTGFAARAAEK
                     LRNERQYCRVISVFIRTSPYSVRDTQYANQATEKLTVATQDSRTIIQAAQAALARIWR
                     EDIAYAKAGVMLADFSGKEAQLDLFDSATPSAGSEALMAVLDGINRRGKNQLFFAGQG
                     IDNSFAMRRQMLSPDYTTDWRSIPIATIK"
     gene            complement(2594..3034)
                     /gene="mucA"
                     /locus_tag="RHZ76_004199"
     CDS             complement(2594..3034)
                     /gene="mucA"
                     /locus_tag="RHZ76_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107685442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit MucA"
                     /protein_id="HDV2814718.1"
                     /translation="MKVDIFESSGASRVHSIPFYLQRISAGFPSPAQGYEKQELNLHE
                     YCVRHPSATYFLRVSGSSMEDGRIHDGDVLVVDRSLTASHGSIVVACIHNEFTVKRLL
                     LRPRPCLMPMNKDFPVYYIDPDNESVEIWGVVTHSLIEHPVCLR"
     gene            3447..3872
                     /locus_tag="RHZ76_004200"
     CDS             3447..3872
                     /locus_tag="RHZ76_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDV2814719.1"
                     /translation="METIEITARYISENARMNFMPAAFRGAFFSADHFIQSFLNRYAK
                     DYQGGYWEYLQASNGAFFMEAPQPLWLSLPNYFEGECSAREVGIIVCLYAYSYFCGLA
                     YEEGKAELNETMANRYHLLREYVNTLENESQNRIYRAID"
     gene            3999..4334
                     /locus_tag="RHZ76_004201"
     CDS             3999..4334
                     /locus_tag="RHZ76_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020320118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdR"
                     /protein_id="HDV2814720.1"
                     /translation="MFDYRNSDQERYGQQIYHHYRKQGNHRWDTSVHQDSGGQYAIIF
                     RHSFSKKQADGVKRTMIRDETVIRAGTAQELTEATFPDFQDSDILKASDFFKSLIQRK
                     AADVTQTDI"
     gene            4344..4583
                     /locus_tag="RHZ76_004202"
     CDS             4344..4583
                     /locus_tag="RHZ76_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HDV2814721.1"
                     /translation="MLNKTENNTNISSINPCSTESLIAVTGNDGETHIIQRELISHSV
                     EGHEATIIHLVTRKSIRVSLSSEILAKKLGITVSE"
BASE COUNT         1230 a         1322 c         1245 g         1265 t
ORIGIN      
        1 gagtatatcc tttctgtaac caaaactacg gaggcgccac agatggctca gaaaaacaga
       61 atttcagaaa cagaatggaa acagctgctg ccgcaaatgg cttcctttgc acacatcaca
      121 acagatattg gctattccgt gcttgtcaaa ggtgagaaat catcagacgt agccacgcga
      181 gtaggccgtt ccaagcaaaa catttccagc accgttaagc gaatctggga tttgtaccag
      241 aacaccactc ttaaagccga gaacggcgaa ccattaaaat tagttcaggt ctggatacca
      301 gcaagccttg ctgaaactgt tttaaaagaa gccgcgaagt acagcattaa taacatcacg
      361 acctccgaaa tggaaaaaaa ataaagtccg aaaggacttt attttaagac tactaacgcg
      421 gctttattac agtgctcgcc accacagtaa accactatcc ccggtttacc ccagagattg
      481 atatgacaca ccgggcagct gtacttttcc cggtttgttt tgttcaccgg cgcttcaggc
      541 ggcggggtta caaatcgcat ggcctctatg atctcgtcgt cgagttcaac cgggtcgcgc
      601 ccttcctcca cctcctgttc gtgttcgcta tcatcttcct cgtcgtcaat atagcctttt
      661 cctgtagggc ttagcacagc gccaggctta ggctggtaag gcggaaacct gtccatccag
      721 gaaagccgga aatggcctgt cagcagttca tcacaggcca tatcgaaagg gccaccggca
      781 ataatataat gggtcatgct ctggcccact ttcctgcctc ccggttcgcc ggtatcagaa
      841 ggcattagtc ctacccgttc catccgggcc gcccactgtt tgttgtgata gccacggcgg
      901 ccaggctcgc caaaatggaa ttgccactga tgaaccatct catgcaccag tgttgaaagc
      961 gtggccttta tggttctgat cgagaaatac accggattca tagcgatctc gtctaccgtg
     1021 tacccactct cacggccgac gaaacgctta aaggaacaat agccatacgt tcgcttctca
     1081 cgctgcaacg ttatcaggca tggcggcagc tcgttgctga atagcttctc attgaaaaaa
     1141 tcgtaggctc tctgaagttc atcgtaggtt tcgggtgtag gcaaattcac tgctaacctc
     1201 acatatatgt attgtgcaat acacataatt tagatgatct tttatacaga ggaaacggct
     1261 tttaccgaaa accgcgaaac cgtgccgaaa cttgttacgg ccggttgagg ggttaaccgg
     1321 cccgggtacg gcgccggtaa ttatttgatg gtggctattg gtattgagcg ccagtctgtc
     1381 gtgtaatcag gtgacaacat ctgacgacgc atggcaaagg agttatcgat gccctggcct
     1441 gcaaaaaaaa gctggttctt tccacgccgg tttataccat caagaacagc cattaaagcc
     1501 tcgctgccag ctgaaggcgt agcagagtcg aataaatcaa gctgggcctc cttcccgcta
     1561 aaatctgcca gcatgacccc tgcttttgca tacgcaatat cttcccgcca gatccgcgcc
     1621 agcgcggctt gtgctgcctg aattatcgtg cggctgtcct gggttgccac cgtcagtttt
     1681 tcggttgcct gattggcata ctgtgtatca cgcactgaat aaggactggt acggataaag
     1741 acgcttatga cgcggcagta ttgcctctca ttacgcagtt tttcagctgc gcgcgctgca
     1801 aatccggtga cagcctgctg catgtccgtc agggtttcta cgcgttggcc gaatgagcgc
     1861 gacacaacaa tctgctgctt cgcaggaggg ttttcttcca ggctgaagca agcctcgccg
     1921 cgcaactccc gtaccgttct ttcaagcaca acgccgaatg ttttacggat taggcgcgta
     1981 tctgccctgg ctaaatccag caccgttttg atccccattg tggcgagtgc tttctctgta
     2041 cgatggccga cgccccagac ttccgcaacc ggcaggatgc tcattaattt cttcagtctg
     2101 gcgccatcgt ccagagcaac caccccgcca gtagcgggcc atgtttttgc agcgtggtta
     2161 cacagcttcg ccagcgtctt agtacgggca ataccgaccc cgcataccag cgttgtgtgt
     2221 cgcctgactt cctcgcgcag ttggcgcccg aaagcgtcaa ggctcatggc ggccgttatc
     2281 cctttgcagt caacaaaaag ctcgtcgatt gaatactgtt cgacgtggct tgcaagggac
     2341 tcaactacgg ccgcgaaccg ggccgacatg gaggcataaa gagtgtaatt agagctaaaa
     2401 atagcgatgt tatgacgctc gattatgggt ctgactttga agtacggatc gcccattttc
     2461 aggcccgctt tcttcgccag gtaattacgg gccacaatgt tgccgtcatt gttggataaa
     2521 acggccactg ctcggtttgc cagatctggc ctaaatgcct gctcacagct ggcgtacatg
     2581 ccattgacat caatcagcgc aaacataccg gatgctcgat aagggaatgc gtaaccactc
     2641 cccagatttc aacgctctca ttatccgggt caatgtagta cacaggaaaa tctttgttca
     2701 tcggcatcag gcagggtctg ggcctcagca gtagtcgctt cacggtaaat tcattatgga
     2761 tgcaggcgac tacgattgag ccgtggctgg ccgtcagcga gcgatccaca accagtacgt
     2821 caccatcatg gatgcggcca tcttccattg acgagccaga aacccgcagg aagtaagttg
     2881 ctgaagggtg acgaacacaa tactcatgca ggtttaactc ctgcttttca tagccctggg
     2941 ccgggctggg gaaccccgca gaaattcttt gcagataaaa agggatgctg tgtacccggc
     3001 tggcgccgga gctttcaaaa atatcgacct tcatagcgat cttccaaata actgtttatt
     3061 tatacagtat cgctgttatg gtcaggttgg caagttttac aggttctcta ctatatgtag
     3121 tgctgcttca gctgccaacg aacgctatat atagtgtttg ttggctttct gctttctcag
     3181 tgtcccctgt aaccggccgc gtggcggcgg gctagcagga aggggggtgg cctgtcgctc
     3241 cgatcagtcc cgcagtttcc ggcgatttgc ccccggtgtc cggtggagtc cggcgaggtc
     3301 gccttccatg ccctgactgg cataaaatag gtaaataaat tccgtacttg tattgacctt
     3361 taaggcataa tcaccgataa ttaaatcatc gggaggcaat accgccgccc gaaaaattgc
     3421 cggtatcggc ttaaagagga agtatcatgg aaactatcga aatcaccgcc cgctacattt
     3481 ctgaaaacgc ccgcatgaac ttcatgcctg cggcatttcg tggcgctttc ttcagcgcag
     3541 atcacttcat tcagtccttt ctgaatcgtt acgctaaaga ctaccaggga gggtattggg
     3601 aataccttca ggccagcaat ggcgcgtttt ttatggaagc gccccaacct ctttggctct
     3661 cgttacctaa ctattttgaa ggtgaatgca gcgcgcgcga agtagggatt attgtttgtc
     3721 tgtatgccta cagttacttt tgcgggctgg catatgaaga aggcaaagcc gaactgaacg
     3781 aaacgatggc taaccggtat cacctgttgc gggagtacgt taatacgctg gagaacgaaa
     3841 gccagaatcg tatttatcgt gccattgatt aactgataac caggcgggca aagcccgcct
     3901 tatcgtgact gtcacgctca ggggaaacta tgttagcgac acttcaacac cctaccgtct
     3961 ggcaattgcc agatcggtta atgcttttgg aactgttaat gttcgattac cgaaattccg
     4021 atcaggagcg ctacggccag caaatctatc accactatcg taaacagggt aatcatcgct
     4081 gggatacttc tgttcatcag gatagcggcg gccaatacgc gattattttc cggcactcct
     4141 tcagcaaaaa acaggctgat ggcgttaagc gaacgatgat ccgcgatgaa accgttatac
     4201 gggcaggtac ggcgcaggag ctgacagaag cgacatttcc cgacttccag gatagcgata
     4261 ttctgaaggc gtcggatttc tttaagtcgc ttatccagcg caaagcggca gacgtaacac
     4321 aaaccgatat ttgaggatca gcgatgctga acaaaaccga aaacaacacc aacattagta
     4381 gtatcaaccc ctgttctaca gagagcctaa tagccgtgac gggaaatgat ggagagacgc
     4441 atatcattca gcgtgagtta atatcccact ctgttgaagg gcatgaagcc acaatcattc
     4501 accttgtaac caggaagtcg ataagggttt ctttgtcatc tgaaattctg gcaaaaaaac
     4561 taggcattac cgtgtctgaa taacgggttt tgttgcttgt tataaggttt tatctgtgtg
     4621 tggcagagtt ctaaaaaggg aaacgtgaga gattctgaat acgattagta taacggaaat
     4681 cgtgttgagt gtgcttcttg cgggcgcttt ggggccggtt ttattacgtc gaaaaaaagg
     4741 tctgctgtta ctgttgttga ttctggtctg cacgatactt ttgcttcatg gtgtcactac
     4801 gatttactga tgtggtcgaa aatctttaaa agcgaacccc cggacgtcac ccctccgggg
     4861 ccgttctgct gtgctgcgca tcgagccgct acgcgtctcg ccccgctcac gcgggcagct
     4921 gccactgacg cctccggcct gcggcctccg cgctccgctt ggcaacccct tcccctgctg
     4981 ccgctcattt ccctttcagc ctcctatcat gattcgtctc ctgcattatc cgtggattgt
     5041 gcagctcagt ggggcgctgg cc
//
LOCUS       DAPHBJ010000252         5049 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.254, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000252 DAPHBJ010000000
VERSION     DAPHBJ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.254"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..546
                     /locus_tag="RHZ76_004203"
     CDS             <1..546
                     /locus_tag="RHZ76_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814722.1"
                     /translation="INKDNIKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQR
                     ITTFFHTSPDVKNREIKLEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDE
                     IQIPSAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIV
                     ISVMFCLVKKMVDEYQQNSGQ"
     gene            complement(789..1484)
                     /locus_tag="RHZ76_004204"
     CDS             complement(789..1484)
                     /locus_tag="RHZ76_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HDV2814723.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1477..2904)
                     /locus_tag="RHZ76_004205"
     CDS             complement(1477..2904)
                     /locus_tag="RHZ76_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HDV2814724.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2915..3634)
                     /locus_tag="RHZ76_004206"
     CDS             complement(2915..3634)
                     /locus_tag="RHZ76_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDV2814725.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(4161..5015)
                     /gene="rclR"
                     /locus_tag="RHZ76_004207"
     CDS             complement(4161..5015)
                     /gene="rclR"
                     /locus_tag="RHZ76_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HDV2814726.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRLAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSRETLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT         1349 a         1324 c         1073 g         1303 t
ORIGIN      
        1 atcaataaag acaatataaa agtgatttac agcctaaaaa taaaccagac aacgccaggt
       61 atacttgtta gctgtgatag caataataat tttgcctgtc agaccacaat gactgaagat
      121 gttatacagc gaattactac attttttcac accagcccag atgtcaagaa cagagaaata
      181 aagctggaat ggtcaggaga taagagagat ttaccaactg ctgaagcgga aatatctcgc
      241 gtgcaggcgt ctattatcaa atggtatgcg tcagaatacc ataatggcag gcaagttctc
      301 gatgagatac aaataccttc agcaattaac agtgagcttt atacaaaaat gatatacctg
      361 accaggaact ggtcattgta tccgaacggt gatggctgtg taactataag ctcaccagaa
      421 ataaaaaata aataccctgc tgccatttgc ctggctctgg gattttttct aagcattgta
      481 atttctgtaa tgttttgcct tgtcaaaaaa atggtagatg aataccaaca aaactctggg
      541 cagtaataaa caacattctg atagagagtg cctgtataaa gctgaagtga caatgaccgg
      601 gaagggaccc cgttttcagg aatggatgcc tgtctttttc actgtaaata tcctggcatt
      661 ggtaatacca ggatatttac agaagagcag cataaccatg acggaacggg attaagtcag
      721 gttgtcctaa acgctatttt caacctcgta tgccttcttc aggtttatgt ccagacttca
      781 tacctctctc agcaatcctc aataatcaga tacaccgctt tcaccggacc atgaaccccg
      841 acgactttga taagctcaat gtccgccgtt gagctgggac cactgatgat gttaatacat
      901 gacggcattc gttcaccggc ctgcgctttc tgatgcaatt tttctgcgag ttgcgctaca
      961 cgcggcagga ttgtgctttt acgcaggata aaaagagaag attccgggag caggctcaat
     1021 gaacgcccgc gctcggcggc ggaaaaaaga accacgcctc ccgattcggt taaaccatat
     1081 tcagcataca caacacccac tttagcctgc tctgcctgcg agatattctc ggcacctttc
     1141 gccggatccc aaacaacggc attgcattcc tgctgcaaac gttcactaat ccccaattcc
     1201 tccagcctcg tgtcaccgct aatcacgacc gactgatctc ccagctcttt acacagacgt
     1261 attgcagctt ctgccgcctt cgcctcgctg gtcagctcac agcgcgtcaa cataacatcg
     1321 ctggcaaact gaataaacgc gtcacagcgc tgctgttggt taagttgggt aagccgctcg
     1381 ttagcatagt tgttaagcgg cgcatcttct gcttgcggtt caagtcgcag cgggcgaccc
     1441 agtgcctgag caacgttatt caaaaattcg cctcgattat ccattctttt tctcctgcgc
     1501 ctgatgtttc ttaaaccaac tacggaaact ctctccgtca gcttcaggaa gatcgcgtgc
     1561 ttccatccag tcgctaatcg cgccaaattt gagtggtgtt ttgccgccat tgataaacca
     1621 gcttgccgca tgagcgccgg ccatcatccc gactttccac aatcctggat gactattggc
     1681 ataagcgaac atttttatcg cccgttgctc tgcttttgcg gtgatccctt tttcagccat
     1741 cacccgacga tgacgcaaaa tcagttttga cagcggaata cgcaccggac acacgctgtc
     1801 acaagcagtg cataaagagc aggcgtaggg taaatcttta aaatctttat agccgccaag
     1861 tagcggagaa atcaccgcac caattggccc tggataaata gagccatatc catgaccgcc
     1921 aatatggcga tatgccggac aagtattcat acaagccccg cagcgaatac agcgcagcac
     1981 atcccgaaat tcagaggcca gcacctcaga acgcccgtta tcgacaataa ccagatgaaa
     2041 ctcttcagga ccatcaacgt gccccgcttc gcgcggtcct gtcagccagg tgttgtatcc
     2101 cgtcaaacgt gcaccaacgg cactgcgcgc cagcatggtg atcaatacat ctacctcggc
     2161 aaacgtgggg gcaatacgct ccattcccat cactgcaata tgcgttttag gcagcgtggt
     2221 acacattcgc gcattacctt cattggtcac caggcatacc gaacctgtct ctgccaccgc
     2281 gaaattacag ccggtaatac ctatttcagc actgaggaaa tcttcgcgga ttttttgccg
     2341 gatgaataag gtcatcgctt caggcgtttc cggcccctca tagcccagac gttcatgtag
     2401 cactcgacgg atctgatggc gatctttatg aattgccggg accacaacat gagatggcgg
     2461 atcttgatcc agctggagaa tatattcacc cagatcggtt tcaatcacct gaatgccagc
     2521 atcctgcaac acatgattga caccaatctc ttcggtcacc atcgatttag atttcaccac
     2581 cttccgggca tttttgcgtt gggcaacctg taaaatgtag cgggtagcgt cttctttggt
     2641 ttttgcaaac cagacgtgac cgccgttttg cgtcactttt tctgagagct ggtacagata
     2701 agcgtcgaga ttactcagaa catgatcacg tatctgggcg gcccgatcgc gccactcctc
     2761 ccagtgcccc aattcatcga ccattttttg ccgatttgcc ccaatacgct gctgcgcgtt
     2821 tgccaccgct ttgcgcatga tcggatcttc aatttgctga cggatgcgtg tcttaaaatc
     2881 tgtattactg gttttgatcg acatctttat atcctcagcg gctcatcaac acttcagcaa
     2941 tatgcatcac tttgactttc tgcccttccc gttgtaatcg cccactgatg ttcagcaggc
     3001 aactcacgtc agcaccaatt aaatactcag ggcggacttc catcaggtgc acaacctttt
     3061 ctttcaccat ctcgccggat atttcggcca ttttgaccga gaacgtgccg ccaaatccgc
     3121 agcaggtatc ctgttcagca aaggtcaaca gctccagtcc acgcacattt ttcagcagcg
     3181 taagtggctc gtccttcact cccagcttac gggccaggct acaagatggg tgatacacag
     3241 ctctcccttg caaactggca cctacatcga ctacccctaa tttattaaca ataaaagagg
     3301 tgagatcctg catacgcgcg gcaacctttt cggcacgtga tgcccattca ggttcatccg
     3361 ccagatacgt cgggtaactt tttacggcat aggtgcaaga gccagccggt gaaataatgg
     3421 gatcgtcgtt atcctccagt gcggcgatca gatttttcat ccctggaatc gcttctttga
     3481 tataaccgct attgatcgca ggctgaccgc agcatccctg tttctccggg aaatttacgc
     3541 gacagccgag tttttccagt agcagcacgg agtctcgtgc cattcttgat ttcagggcgt
     3601 caccaataca ggtgacaaag aaattgacat tcacaactaa tactccatta cttcatgccc
     3661 atttatgccc gatgtttccc ccccaccggg ggatcaatca tcaataaata tggcgttctt
     3721 ttttaattgg ctagcaacat accacccctc atttgtatga caaatgataa tctttcatcc
     3781 aaacgtcaaa tgcaataact aattttattt ctttcggcat cgctcacatt ttatggggaa
     3841 atcgacgatc atttttggca taagaaatcc atttaaaaat ggctacatcc cttataccta
     3901 ttcagagcta cttattcagg ggttattgag atatacactg atggtgtaca aaaaaatcac
     3961 cgacaataat cacgtatcaa gtaatagtcg ggattttatt taattgaagc aatttagttt
     4021 gcatttttat ttaaaaactg agctagagtc catcagaaaa aatatataaa gcccaaacca
     4081 ccgttggaaa tagaaatagt tgaaaggctc acaactcccg gaatgcggat tatctttgcc
     4141 ctgatttctg gcggttttat tcagggtgca agctgtctga ccctttcccg atattctccc
     4201 ggggtacaac caaactcgcg gacaaacgcc ttgtgaaaag atgattcact ggcatagcct
     4261 actgactcag cgatcaccac aacagggagc gtttcccggg aaaacatctg ggccgctatt
     4321 tgtagacgca actttgttaa tacagccagc ggcgtggttc cggaaacatc acggaaaagc
     4381 tgggcaaaac ttgcccggga catgtgggcg atgctggcca gcgattcgac ggtccaggcg
     4441 tgtcctggca tttccaacat ttgctgtatt accgcaccaa gacgtggatg cagaagcaaa
     4501 ctgagaatgt ttttctcagt attaacctgt gcaatccact cacgcaccgc gagggtaaag
     4561 aatgtagcgc agatctggct acacagtgca tccactcccg gcattgccaa tctggattcc
     4621 tgttgtaaaa acgggatcgc ctccctcagc cagttatatt ctacgctgtg gttaaccggt
     4681 gctaaaaaca gcgtttccgg caaagacgtt aaaaaataac gcgccgaatg ttgcagccga
     4741 agagtgccgc agacaataca ggttgattca ttatcgacat gactcaggcg atgggctgaa
     4801 ttttgtggta gcaggaccac atttcccggg cgtaatgtaa aaatctcccc cgtcggcatt
     4861 tccagcttcg ccgctccttg cgttaacgca tgccaacgaa taaccgataa ttccccggca
     4921 ccatgtggaa gctgccagtc acttcctaac acgcaattct tatcgatcgt tccttgtgga
     4981 gcgttaagca tcaacaaacg gctaagggca tccatatcag actcctgagt ggtttttgat
     5041 tacaaacac
//
LOCUS       DAPHBJ010000253         4887 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.255, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000253 DAPHBJ010000000
VERSION     DAPHBJ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.255"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            48..404
                     /locus_tag="RHZ76_004208"
     CDS             48..404
                     /locus_tag="RHZ76_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HDV2814727.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDNESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQRVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            504..1709
                     /locus_tag="RHZ76_004209"
     CDS             504..1709
                     /locus_tag="RHZ76_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2814728.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYIAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISMLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRRHHS
                     LKENVAPGG"
     gene            1936..3201
                     /locus_tag="RHZ76_004210"
     CDS             1936..3201
                     /locus_tag="RHZ76_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2814729.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTQLNTIKHRG"
     gene            3213..4079
                     /gene="ydiB"
                     /locus_tag="RHZ76_004211"
     CDS             3213..4079
                     /gene="ydiB"
                     /locus_tag="RHZ76_004211"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HDV2814730.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVY
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            4110..4868
                     /gene="aroD"
                     /locus_tag="RHZ76_004212"
     CDS             4110..4868
                     /gene="aroD"
                     /locus_tag="RHZ76_004212"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDV2814731.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
BASE COUNT         1231 a         1097 c         1132 g         1427 t
ORIGIN      
        1 ataatgttat ttatatttta ttgtttattt catgcagcca ttacacgatg aacgcttatg
       61 aactccaggc tcttcgccat atttttgcta tgactattga tgaatgcgcg acctggattg
      121 cccagacagg tgacaatgaa agctggcgtc agtgggaaaa tggcaaatgc gccattcctg
      181 atcgtgtggt cgagcaactg ttggctatgc gacagcaacg aaaaaaacat cttcatgcca
      241 tcatagagaa aataaataac cgcatcggca acaacaccat gcgttttttc cccgacttaa
      301 ccgcgtttca acgtgtctac cctgatggca attttattga ctggaagatc tatcaatcgg
      361 tagccgctga attatatgca cacgatctgg agcggctttg ttaatttttc cacagaaagg
      421 aattgtcgtt gttacaacaa taatgaatgg atactgacac aacatcgcta cactttttaa
      481 agcacctttg ctaagtagaa cctatgaaaa atccctatta ccctaccgca cttgggttgt
      541 attttaatta cctggtgcat ggtatgggcg tcattttgat gagcctgaat atggcctcgc
      601 tggagacgct ttggcagact aatgccgcgg gcgtctcgat agttatctca tcgctgggca
      661 ttggtcgatt aagtgtcttg ctttttgcag gattattatc cgatcgcttt ggtcgccgcc
      721 cttttatcat gctcgggatg tgctgctata tagccttctt ttttggcatc ctgcacacca
      781 ataacatcat tatcgcttat gtttttggct ttctggcggg aatggcaaac agttttctcg
      841 atgcaggcac ttatcccagt ttgatggaag cttttccacg ctcaccggga acagccaata
      901 ttttaattaa agcatttgtt tccagcggac aatttttatt accgctaatc atcagcatgt
      961 tagtgtgggc tgaattgtgg ttcggttggt cctttatgat tgctgcaggc attatgttta
     1021 ttaacgctct gtttttatac cgttgtacgt tcccacccca tccgggtcgt cgcttacctg
     1081 tcataaagaa aaccaccagc tctacggaac atcgctgttc aattatcgat ttagccagtt
     1141 ataccttata tggctatatc tcaatggcaa cgttttatct ggttagccag tggctggcac
     1201 agtacggaca atttgttgca ggcatgtcat acactatgtc gatcaaacta ctcagtatct
     1261 acaccgtggg ttcgctgctt tgtgtattta ttaccgctcc actcattcgt aataccgttc
     1321 gcccaacaac attactcatg ctgtacacct ttatctcatt tattgctctg tttaccgtct
     1381 gcctgcatcc cacattttat gtggtgataa tatttgcttt tgtcattggt tttacctctg
     1441 ctggaggtgt tgtgcaaatt ggcctgacgt taatggctga acgtttccct tacgctaaag
     1501 gtaaagctac agggatctat tacagtgcgg gcagtattgc gacctttact attccgttga
     1561 ttacggctca tctgtcccaa agaagtattg ccgatattat gtggttcgat accgccatcg
     1621 ctgccatcgg ctttttactg gcactgttta tcggcttacg cagccgccgg catcactcgc
     1681 taaaggaaaa tgtcgctccg ggtgggtaat gcaatattct tttcaggtca tgcaagatct
     1741 tacggataaa taactctttc tgcgctaact aaggaaaatc gcgatcaaaa acaaattatg
     1801 atatgcaata ttcctggaaa cataaacttt atgccatgta cccagggaaa atcatcttca
     1861 gtatagtaat tatgtaaacc gtcggagaac aatacgtacg gtaacgaaat aatctttcag
     1921 caaggagctg tgaaaatgtc tcaaaataag gctttcagca cgccatttat cctggctgtt
     1981 ctttgtattt acttcagcta cttcctgcac ggcattagtg ttattacgct tgcccaaaat
     2041 atgacatctc tggcagaaaa gttttccact gacaatgcgg gcattgccta tttaatttcc
     2101 ggtatcggtt tggggcgatt gatcagtatt ttattcttcg gtgtgatctc cgataagttt
     2161 ggtcgtcggg cggtgatatt aatggcagta ataatgtatc tgctattctt ctttggtatt
     2221 cccgcttgcc cgaatttaac tctcgcctac tgtctggcag tgtgcgtagg tatcgctaac
     2281 tcagcgctgg atacgggtgg ctaccccgcg ctcatggaat gctttccgaa agcctctggt
     2341 tcggcggtca tactggttaa agcgatggtg tcattcggac aaatgttcta cccaatgctg
     2401 gtgagctata tgttgctcaa taacatctgg tacggctatg ggctgattat tccgggtatt
     2461 ctgtttgtac tgatcacgct gatgctattg aaaagcaaat tccccagcca gttggtggac
     2521 gccagcgtag ctaatgaatt accgcaaatg aacagcaaac cgttagtctg gctggaaggt
     2581 gtttcatcgg tactgttcgg tgtagccgca ttctcgactt tttatgtgat tgtggtgtgg
     2641 atgcccaaat atgcgatggc ttttgctggt atgtcagaag ctgaggcatt aaaaaccatc
     2701 tcttattaca gtatgggctc gttggtctgt gtctttattt ttgccgcact actgaaaaaa
     2761 atggtccggc ccatctgggc taatgtattt aactctgcac tggcaacaat aacagcagcc
     2821 attatctacc tgtacccttc tccactggtg tgtaatgccg gcgcctttgt tatcggtttc
     2881 tcagcagctg gcggcatttt acagctcggt gtttcggtca tgtcagagtt tttcccaaaa
     2941 agcaaagcca aagtcaccag tatttatatg atgatgggtg gactggctaa ctttgttatt
     3001 ccgctgatta ccggttatct gtcgaacatc ggcctgcaat atatcattgt tctcgatttt
     3061 actttcgcgc tgctggccct gattaccgca attattgttt ttatccgcta ttaccgcgtt
     3121 tttattattc ctgagaatga tgtgcggttt ggcgagcgca aatttagcac ccagttaaac
     3181 acaattaagc atagaggtta aaggagttaa ttatggatgt taccgcaaaa tacgaattga
     3241 ttgggttgat ggcttatcct atccgccaca gtttatcgcc tgaaatgcag aataaagcct
     3301 tagaaaaagc gggattgcca tttacctata tggccttcga agtggataac gatagctttc
     3361 ccggagcaat tgaaggatta aaagctctaa aaatgcgcgg aactggtgta tcaatgccga
     3421 acaaacaact ggcgtgtgaa tatgttgatg aattaacgcc agcagccaaa ctggtgggtg
     3481 ccatcaacac catcgttaat gatgatggct acctgcgcgg ctataacacc gacggcacgg
     3541 gccatattcg cgccattaaa gagagcggtt ttgatatcaa aggcaaaacg atggtgctgt
     3601 taggggccgg aggtgcctca acggcgattg gcgcgcaggg ggcaattgaa ggtttaaaag
     3661 aaattaaact ctttaaccgt cgggatgagt tcttcgataa agccctcgcc ttcgcgcagc
     3721 gggttaacga aaacaccgat tgtgtcgtca cggttaccga tctcgccgat cagcaagcct
     3781 ttgctgaagc cctggcttcc gccgacattt taaccaatgg cacaaaagtg ggtatgaaac
     3841 cccttgagaa tgaatcattg gtttatgata tcagtctgtt acatccggga ctgctggtca
     3901 ctgaatgcgt gtataacccg catatgacga agttattgca gcaggcacaa caagctggtt
     3961 gcaaaacgat tgatggatac ggcatgttgt tgtggcaagg ggctgaacag ttcacgttat
     4021 ggactggcaa agatttccct ctggaatatg ttaaacaggt catggggttc ggtgcctgac
     4081 aggctgaccg cgtgcagaaa gggtaaaaaa tgaaaaccgt aactgtaaaa gatctcgtca
     4141 ttggtgcggg cgcacctaaa atcatcgtct cgctgatggc gaaagatatc gccagcgtga
     4201 aatccgaagc tctcgcctat cgtgaagcgg actttgatat tctggaatgg cgtgtggacc
     4261 actatgccga cctctccaat gtggagtctg tcatggcggc agcaaaaatt ctccgtgaaa
     4321 ccatgccaga aaaaccgctg ctgtttacct tccgcagtgc caaagaaggc ggcgagcagg
     4381 cgatttccac cgaggcttat attgcactca atcgtgcagc catcgacagc ggcctggttg
     4441 atatgatcga tctggagtta tttaccggcg atgatcaggt taaagaaacc gtcgcctacg
     4501 cccacgcgca tgatgtgaaa gttgtcatgt ccaaccatga cttccataaa acgccggaag
     4561 ccgaagaaat cattgcccgt ctgcgcaaaa tgcagtcctt cgacgccgat attcctaaga
     4621 ttgcgctgat gccgcaaagt accagcgatg tgctgacgtt gcttgccgcg accctggaga
     4681 tgcaggagca gtatgccgat cgtccaatca tcacgatgtc gatggcaaaa actggcgtaa
     4741 tttctcgtct ggctggtgaa gtatttggct cggcggcaac ttttggtgcg gtaaaaaaag
     4801 cctctgcgcc agggcaaatc tcggtaaatg atttgcgcac agtattaact attttacatc
     4861 aggcataagc aataatattt cggcgga
//
LOCUS       DAPHBJ010000254         4858 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.256, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000254 DAPHBJ010000000
VERSION     DAPHBJ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.256"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            77..1993
                     /gene="mngA"
                     /locus_tag="RHZ76_004213"
     CDS             77..1993
                     /gene="mngA"
                     /locus_tag="RHZ76_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HDV2814732.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHVLTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLWVIGSFVLGSMVTG
                     AIVGAMNIGLSTSGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLMWRRH
                     AVKHGNYLTDGVMP"
     gene            2011..4644
                     /gene="mngB"
                     /locus_tag="RHZ76_004214"
     CDS             2011..4644
                     /gene="mngB"
                     /locus_tag="RHZ76_004214"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDV2814733.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLIRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YDMAVPLNLSERSARQSTGRVGVVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDVMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     VSNLSGPFLPGQSRTFSYRLA"
BASE COUNT         1181 a         1140 c         1340 g         1197 t
ORIGIN      
        1 tgaaattgat cacataatgg tattgtttta tcgggcacgc tggcgcgact ataaaaacga
       61 tcaagtgagg atcatgatga atctgacgac tctgacccac cgcgatgcgt tgtgtctgaa
      121 tgcgcgcttt accagccgtg aagaggccat ccacgtgttg actcaacgtc ttgctgctct
      181 ggggaaaatt tccagtactg agcaatttct ggaagaagtg tatcgccgtg aaagccttgg
      241 cccgaccgcc ttaggtgaag ggttggctgt gccgcatggc aaaactgctg cggtaaaaga
      301 agcggcgttt gcggtcgcca cactcagcga gccgcttcag tgggaaggcg ttgatggccc
      361 ggaagcagtt gatttagtgg tgctgctggc gattcccccc aatgaagcgg gtactacgca
      421 tatgcaactg ctgacagcgc tgaccacgcg ccttgcggat gatgagattc gggcgcgtat
      481 acagtcggcg acgacgcctg atgagttgct ctcggcgctg gatgacaagg gaggcacgca
      541 accttctgcc tctttttcca acgcgccaac tatcgtctgc gtaacggcct gtccggcggg
      601 tattgctcac acctatatgg ctgcggaata tctggaaaaa gccggacgca aactcggcgt
      661 aaatgtttac gttgaaaaac aaggcgctaa cggcattgaa gggcgtttaa cggcggatca
      721 actcaatagt gcaaccgcct gtatttttgc ggctgaagtc gccatcaagg agagtgagcg
      781 ttttaatggc attcccgcgc tttcagtgcc tgttgccgag ccgattcgcc atgcagaagc
      841 gttgatccaa caagcgctta ccctcaagcg tagcgatgag acgcgtaccg tacagcaaga
      901 tacgcaaccg gtgaaaagtg tcaaaacgga gctgaaacag gcactgttga gcggaatctc
      961 ttttgccgta ccgttgattg tcgcgggggg cacggtgctg gcggtcgcgg tattactgtc
     1021 gcaaatcttc gggctacaag atctgtttaa tgaagaaaac tcctggctgt ggatgtaccg
     1081 caagctgggc ggcgggctgc tcggaatttt gatggtaccg gtgctcgcgg cctataccgc
     1141 ctattctctg gcagataaac cggcgttagc gccaggcttt gcggctggac ttgccgccaa
     1201 catgatcggc tccgggtttc tcggcgcggt cgttggcgga ttgatagccg gttacttgat
     1261 gcgctgggtg aaaaatcact tgcgtcttag cagtaaattc aatggattcc tgacttttta
     1321 tctctacccg gtgctcggta cgttgggagc gggcagtctg atgctgtttg tggtggggga
     1381 acctgtcgcc tggatcaata actcgcttac cgcctggctg aacggtctgt caggaagtaa
     1441 cgcgctgttg ctgggtgcca ttctcggttt tatgtgttcc tttgaccttg gagggccagt
     1501 gaataaagcc gcttatgcat tctgcctggg cgcaatggcg aacggcgttt acggcccgta
     1561 tgccattttc gcctccgtca aaatggtttc ggcatttacc gtaaccgctt ccacgatgct
     1621 cgcaccgcgc ctgtttaaag agtttgaaat tgagaccggg aaatccacct ggctgttagg
     1681 gctggcaggt attaccgaag gggcgatccc gatggcgatt gaagatccgc tgtgggttat
     1741 tggttcgttt gtgctgggct ctatggtaac gggcgctatt gtcggtgcga tgaatatcgg
     1801 cctttcgaca tccggtgccg gcattttctc gctcttttta cttcatgata atggcgcggg
     1861 cggtgttatg gcggcaattg gctggtttgg cgcggcattg gtgggggctg caatctcgac
     1921 tgcaattctc ctgatgtggc ggcgtcacgc ggttaagcat ggcaactatc tgactgatgg
     1981 cgtaatgcca taaacaaaaa ggaaacgacg atgaaagcag tatctcgcgt tcacatcacc
     2041 ccgcatatgc actgggatcg agagtggtat ttcaccaccg aagagtcacg tattctgctg
     2101 gtcaataata tggaagagat cctgtgccga ctggaacagg acaacgaata caaatattac
     2161 gtactcgacg ggcaaacggc gatcctcgaa gattatttcg cggtgaaacc ggaaaacaaa
     2221 gaccgtgtga agaaacaggt agaagccggc aagttgatta tcggcccctg gtatacccag
     2281 accgatacca cgattgtttc tgcggaatcc atcgtccgta atttgatgta cggaatgcgt
     2341 gactgcctcg cgtttggcga gccgatgaaa ataggttatt taccagattc ctttggcatg
     2401 tccgggcaac tgccgcatat ctacaatgga tttggcatta cccgcaccat gttctggcgc
     2461 ggatgttcgg agcgccacgg tactgataaa accgagtttt tgtggcaaag cagtgacggt
     2521 agcgaagtga cggcgcaggt gctgccgctg ggctacgcca tcggtaagta cttacctgcc
     2581 gacgaaaacg gattacgtaa acgcctcgac agttattttg acgtgctgga aaaagcgtct
     2641 gtaaccaaag agattttgct gccgaatggg catgaccaga tgccattgca gcaaaatatc
     2701 ttcgaagtga tggataagct acgtgagatc taccctcaac gtaagtttgt gatgagccgc
     2761 tttgaagagg tatttgagaa gatcgaagcg cagcgagata atctggcaac cctgaaaggg
     2821 gaatttattg atggcaaata tatgcgcgtg catcgcacca tcggttctac gcgtatggat
     2881 atcaaaattg cccacgcgcg tattgaaaat aagattgtta atctgctgga accgctggca
     2941 acactggcct ggacgttggg ttttgaatac caccacggct tgctggagaa aatgtggaaa
     3001 gagatcttaa aaaatcatgc ccacgacagt atcggctgct gctgtagtga caaagttcat
     3061 cgcgaaatcg tcgcccgctt cgaactggct gaagacatgg cggataatct gattcgtttc
     3121 tacatgcgca aaattgccga caacatgccg cagagcgacg ccgacaaact cgtcctgttt
     3181 aacctgatgc cctggccgcg tgaagaagtt atcaacacca ctgtgcggct gcgcgccagc
     3241 cagtttaatt tgcgggacga tcgcggtcag cctgtaccgt attttattcg ccatgcccgt
     3301 gagatcgatc caggcctaat cgatcggcaa atagttcatt acggtaatta cgatcccttt
     3361 atggagtttg atatacagat caaccagatt gtcccttcta tgggctatcg cacgctttat
     3421 atcgaagcga atcagcctgg caacgtgatt gcggcaaaaa gtgacgctga agggatactg
     3481 gaaaatgctt tctggcaaat tgcgctcaat gaggatggtt ctctgcaact ggtagataaa
     3541 gacagcggtg tgcgctatga ccgggtattg caaattgaag aaagctctga tgatggtgat
     3601 gaatatgact attcacccgc aaaagaagag tgggtaatta ccgcagcgaa cgcgaaaccg
     3661 caatgcgata ttattcatga agcctggcag agcagggctg ttatccgcta tgacatggca
     3721 gtgccgctca atttgtcaga acgcagcgcc cggcaatcca ctggcagagt aggggtggtg
     3781 ttggttgtca ctcttagtca taacagcagg cgtattgatg tggatatcaa tcttgataac
     3841 caggctgacg atcatcgcct tcgtgtcctg gtccctacac cttttaacac cgacagtgtt
     3901 ctggcagata cgcagtttgg ttcgctaacg cgccccgtga acgacagtgc aatgaacaac
     3961 tggcagcaag aaggctggaa agaagcgccg gttccggtat ggaatatgct caactatgtt
     4021 gccttacagg aagggcgtaa cggcatggct gtctttagcg aagggttacg tgaatttgaa
     4081 gtcatcggtg aagagaagaa aacctttgcc attacgttgc tgcgtggcgt gggcttactg
     4141 ggcaaagaag atctgctttt aaggcctggg cggccttcgg gaattaaaat gccagtcccg
     4201 gactcacaac tacgtggtct gctttcttgt cgcctaagtt tattgagtta taccggtacg
     4261 ccaaccgccg ctggtgtagc tcagcaggcg cgagcatggc tgactccagt acagtgttac
     4321 aacaaaatcc catgggatgt gatgaagctc aacaaagccg gattcaacgt gccggaaagt
     4381 tatagtttgt tgaaaatgcc cccagtggga tgcctgataa gcgcacttaa gaaagctgaa
     4441 gaccgacaag aagtgatttt acggctgttt aatccggctg aatcagcaac ctgtgatgcg
     4501 actgttgctt tcagtcgcga ggtgatttct tgctcagaaa cgatgatgga tgaacacatt
     4561 accaccgagg aaaatcaagt ttcaaatcta tcggggcctt ttttacccgg ccagtcacgg
     4621 acgttcagtt accggcttgc ctgaatagca atcaaaccga agccacatat gcgcggccag
     4681 attgttgaca aagggcgctt tgttcatgcc ggatacggca tgaacgcttt attcggtcta
     4741 caaaagcagg caaattcaat atattgcaga gatgatgtag acactggcaa gcgtagcgca
     4801 tcaggtaatt ttgcgtttat cttcactctc aagccacgta tatgtggctt tattttta
//
LOCUS       DAPHBJ010000255         4850 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.257, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000255 DAPHBJ010000000
VERSION     DAPHBJ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.257"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            4..939
                     /locus_tag="RHZ76_004215"
     CDS             4..939
                     /locus_tag="RHZ76_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDV2814734.1"
                     /translation="MEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVNAA
                     KVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESILGALD
                     IEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQLSQFQTD
                     LIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLLPEEQNF
                     SGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPF
                     PSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            1148..2113
                     /gene="lipA"
                     /locus_tag="RHZ76_004216"
     CDS             1148..2113
                     /gene="lipA"
                     /locus_tag="RHZ76_004216"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDV2814735.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2214..2417)
                     /gene="tatE"
                     /locus_tag="RHZ76_004217"
     CDS             complement(2214..2417)
                     /gene="tatE"
                     /locus_tag="RHZ76_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HDV2814736.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2546..3334)
                     /locus_tag="RHZ76_004218"
     CDS             complement(2546..3334)
                     /locus_tag="RHZ76_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HDV2814737.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3427..3810
                     /gene="crcB"
                     /locus_tag="RHZ76_004219"
     CDS             3427..3810
                     /gene="crcB"
                     /locus_tag="RHZ76_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDV2814738.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3865..4074)
                     /gene="cspE"
                     /locus_tag="RHZ76_004220"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3865..4074)
                     /gene="cspE"
                     /locus_tag="RHZ76_004220"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDV2814739.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(4249..4809)
                     /gene="pagP"
                     /locus_tag="RHZ76_004221"
     CDS             complement(4249..4809)
                     /gene="pagP"
                     /locus_tag="RHZ76_004221"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HDV2814740.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         1286 a         1231 c         1087 g         1246 t
ORIGIN      
        1 caaattgaac cctgtttaag cagaaagtcg tcagaaggta aaccacaaat atttacaacc
       61 ttacgaaata ttgatcttaa ccttctgact atttttgaag ctgtatatgt acataaaggg
      121 atcgttaatg cagcgaaagt gcttaatctg accccctcgg caatcagtca gtctattcag
      181 aaactgcgcg ttatattccc tgacccattg tttattcgca aaggccaggg tgtcactcct
      241 accgcatttg cgatgcatct acatgagtat atcagtcagg gccttgagtc cattcttggc
      301 gcgctggata tcgaaggaag ctatgataag caacgaacga taactattgc gaccactccc
      361 tcggtcggag ccctggtcct tcctgtcatc tatcgggcga ttaaaactca ctatccgcag
      421 cttttactgc gcaacccgcc catcagcgac gcggaaaacc aactcagtca gtttcaaacc
      481 gatctcatca tcgataacat gttttgcacc aatcgtacgg tgcaacatca tgttctgttc
      541 accgacaata tggtgttaat ttgccgcgag ggaaatccac tactctcttt agaagatgac
      601 agagagacta tcgacaacgc tgcgcatgta cttctgttac cggaagagca aaatttcagc
      661 ggtctgcggc agagagttca agaaatgttt ccggaccggc aaatcaattt caccagctac
      721 aacattttga caatcgctgc actggttgcc aacagtgaca tgttagcgat tattcccagc
      781 cgtttttata acctgtttag ccgctgctgg ccgctggaaa aactgccttt tccgtcctta
      841 aatgaggagc aaatagattt ctctatccac tacaacaaat tcagcctgcg tgatcccatc
      901 ctgcacggag taatagatgt tatccgtaat gcattttgaa aaagtattgt tacagccaga
      961 tcacagcgca gaacgataaa atggcacaat ccaacaacaa ttttacattt tcgcgaccgc
     1021 tttggctgct ttcaggtccg tttcaatgat atactgccag tcgttaattc aaaaatagtt
     1081 gataattaca acaatctatt gaattgaaac gctttccttc gtaattcgca actggaacac
     1141 gcacgctatg agtaaaccca ttgtgatgga acgcggtgtt aaataccgcg atgccgataa
     1201 gatggccctt atcccggtta aaaacgtggc aacagagcgc gaagccctgc tgcgcaagcc
     1261 ggaatggatg aaaatcaagc ttccagcgga ctctacacgt atccagggca tcaaagccgc
     1321 aatgcgcaaa aatggcctgc attctgtctg cgaggaagcc tcctgcccta acctggcgga
     1381 atgcttcaac cacggcacag caacgtttat gatcctcggc gctatttgta cccgccgttg
     1441 tccgttctgt gatgttgccc acggtcgccc ggtagctcct gatgccaatg aaccagtgaa
     1501 actggcgcag accattgccg atatggcgct gcgttatgtg gttatcacct ccgttgaccg
     1561 tgatgacctg cgcgatggcg gtgcccagca ctttgcggat tgcattactg ccattcggga
     1621 aaaaagcccg caaatcaaaa ttgaaactct ggtgccggat ttccgtggtc gtatggatcg
     1681 tgctctggat attctgactg caacgccacc agatgtgttc aaccataacc tggaaaacgt
     1741 accgcgtatt taccgtcagg tacggcctgg tgcagattac aactggtcgc tgaagctgct
     1801 ggaacgcttt aaagaagcgc atccggaaat cccgaccaag tctggtctga tggtgggact
     1861 gggtgaaacc aatgaagaaa ttattgaggt aatgcgcgac ctgcgccgtc atggtgtgac
     1921 gatgttaacg ctggggcaat atttgcagcc aagccgccat cacctgccgg ttcaacgtta
     1981 cgttagcccg gatgagttcg acgaaatgaa agccgaagcg ctggcgatgg gctttaccca
     2041 tgctgcatgc ggtccgtttg tccgctcttc ttaccacgcc gatttgcagg cgaaagggat
     2101 ggaagttaag taatcctgta ataattactt acatctgtct gataaaaaac ccgctttttg
     2161 aagcgggttt tttgtatcga acaagatatt gagggagcgt cctgctcgcc acgtcactct
     2221 ttatgagaga gcttttcagc ctgaagatca acgtctgcgc cttttttcgc cgcagcatca
     2281 tcatcattca tcgccttctt gaacccttta atggccgctc caaggtctcc gcccagcgta
     2341 cgtaacttct tagtcccaaa cagcagaacg accagcgccg caactaccag cagtttggta
     2401 atactaatct cacccataga taccttcttg acatataacc ggcaacagaa cgcgccttaa
     2461 aaaccagaga atattaacgg tcattcgacg cgcgcacaca atagcaatct gtaacaaggt
     2521 gaatcaagcg ccgagtaaaa aaacatcata ataattgcgg cggcgcaaag cgacggttgt
     2581 ttaagacggg cagttgcgcg cgcacctgac gcacacgttc gggcgtcact tccgccataa
     2641 tgagtgcagg catttctgac gctgccgcaa tggtgactcc aaagggatct ataatccggc
     2701 tttgaccgat atttttgttc ccgcactccc ccgccgccac catataacag gtggtatcca
     2761 gcgcacgagc ggcaagcaac gttgaccagt gatgctcttt gagcggcccg cgaacccagg
     2821 cggcaggaag taccaggatt tcagctccct gtaatgcctg tgccagcgcc agctctggaa
     2881 agcgtaagtc ataacaggtc atcagaccga ccttcatccc ctccacctcg agtaacggag
     2941 cgatttcatt accagcatca acacggcgtg attcctgaat ggcaaatgca tcatagagat
     3001 gcaatttggc ataacgggcg acgatgtttc ctgcctgtag cgccaccagc atattccatg
     3061 cgcgccccgg cgttgaagga acatgaatcg tcagaattgt cgtcatcatg ttacgtttac
     3121 tttctcgccg taaacgtccg aggaattcgc cttccagcag ctgtgctgat ttaaccgata
     3181 gatctgcatc atgatcatcg cgcgccagca atgcttccgg caggacgaac agcgatacgt
     3241 cgttttccgc cgcctgcgcc atcaacgagg cacaaatctc agcgttcttt tcccacacag
     3301 atgtaacagc aaactgtcct gcggcaacca gcattgccac ctcctgtcag agtcgtaaaa
     3361 ttcgccaacg acgattattc ggcgttacac ttatcactca tacaaatcaa atagcaggat
     3421 tttgcagtgt tacaacttct tttagcagtt tttattggcg gtggtacggg aagcgtggcg
     3481 agatggctgt taagtatgcg atttaacccg ctgcatcagg cgattccgtt ggggacgctg
     3541 gcagcaaacc tgattggggc attcatcata ggaatgggat tcgcctggtt cagcaggatg
     3601 acgaacattg atccagtgtg gaaagtatta atcaccaccg gattttgtgg cggtctaaca
     3661 accttctcaa cattttcggc agaagtggtg tttttgttac aagagggccg ctttggctgg
     3721 gcgttactga acgttttcgt caaccttctg gggtcttttg ccatgacagc actggctttc
     3781 tggctgtttt cggcctcaac cgcacactaa agaccaaaaa aaaacccgct gattaagcgg
     3841 gttttgaatt cttgctgacg tatcttacag agcgattacg tttgcagcag aagggccttt
     3901 ggcaccgtta gtgatttcga actctacgcg ctgaccttca gcaagagttt taaaaccatt
     3961 agtctggatt gcagagaagt gtacgaacac gtctttgctg ccgtcttccg gagtaatgaa
     4021 accgaatcct ttggactcat taaaccactt aacgttacct ttaatcttag acatcaaaat
     4081 tacctttaca tgaaaaatag acacaaatgc tgtgtcggtt accagtacac caattgtggt
     4141 acgctttgtc cagtgcaact ttactaaaaa gtgataaaag tcgcgttatt tttgatgcta
     4201 cttattttag ttattgattt taaagaagtt actaaaactt catttgtctc aaaactgaaa
     4261 gcgcatccag gcaaagtaca cattgccatt gttgtaggta cccggaatgt aggtcatctg
     4321 aaaagtcact gggccataac ccacggaggc caatggcagt agaaccggga gagggatgta
     4381 attccagtta tcgcgtgccg ttacgccagc ggtgaatccc agacctaaat gaaaattttc
     4441 atccgccagc ggtcgccagg tactttccca tccgtatccg gcaatcggtt cccatttgtt
     4501 ccacgagtcc ttaaatgcca tggcatacag gccatgccag tttccttttt catcccaacg
     4561 cgacaggcca aaaccgccac cccacggtcg ctcgttatag cgatcggttt tttctttgtc
     4621 gtaagcgaaa cgtgcatgcc aggtgatggc aggaatatat aaatcataat gttcaggctg
     4681 ttgccaggtt tgtgcaatat tttctctaaa cgttgtcatc cactcatctg cgttagcaaa
     4741 aactttacca acgctgatta actgaataaa aacaaaggaa aagatagcga catatttact
     4801 cacgttcatt tgtgaccata aaacatttat caaaaatcta ctactagcat
//
LOCUS       DAPHBJ010000256         4805 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.258, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000256 DAPHBJ010000000
VERSION     DAPHBJ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.258"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            204..872
                     /locus_tag="RHZ76_004222"
     CDS             204..872
                     /locus_tag="RHZ76_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814741.1"
                     /translation="MAASRITHLITSCTKGKHSQCGSMPELSIRSGQTPEEAMSSWAA
                     TIKRSQSASPVPALSLYAGNHWSTAKEILRTTENLELWVISAGLGFLNSRDLVDAYEA
                     TFHDLPFSHRQWWRELTNTFGKERTAKSIETLMAARPFDDYVIAASPVYIEATEDDIL
                     AGASKLNNHIAQLTVVTSGEYSGLLESYLIRSESRMMRQLSSNMVCLNIKLAQYIIGS
                     RRYS"
     gene            complement(1095..4103)
                     /locus_tag="RHZ76_004223"
     CDS             complement(1095..4103)
                     /locus_tag="RHZ76_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HDV2814742.1"
                     /translation="MAADFLTDKQTQNYGRYAAEPNEIQLARYFHLDERDLTFINLRR
                     GRHNRLGIALQLTTARFLGTFLPDLMQIPPGVQFYVARQLNIRYPEIISRYAQRENTR
                     WEHHGLIRQHYSYHDFGDFPWSFRLKRLLYTRAWLSNERPGLMFDFATAWLLQNKVLL
                     PAASTLTRVIGEIRERATRRLWRKLAALPNRWQAAQLAGLLEIPEGQRLSVMEHLKRG
                     PVTISGPAFTEALERYTRLRSLEFSCLNFTGLPAIQLRNLARYAGMASVKYISRMPEE
                     RRLAILTAFVKAQEISALDEAVDVLDMLILNITREAKKTGQKKRLRTLKDLDRAALLL
                     ARACALLLDEDTGDDLLRKTIFSSVPVARLAESVEKVNELARPQDTNFQDEMVEQYGR
                     VRRFLPALLRDLHFRAAPDGEHTLAAIHYLAELNGSKKRILDDAPEHIISGPWKRLVY
                     DAEGRIQRAGYSLCLLERLQDALRRRDIWLENSDRWGDPRQKLLQGEEWQAQRVPVCR
                     ALGHPTNGSKASVQLAARLDETWKTVASRFDRNTAVDICNEGKHPSLTISSLDKLDEP
                     PALIRLSSRVRQLLPPVDLTELLLEIDARTGFTREFSHVSESGARAQDLHIILCAVML
                     AEACNIGHEPLIKHNIPALTRHRLSWVKQNYIRAETLVSANARLVDFQSSLALAGYWG
                     GGEVASADGMRFVTPVKTVNSGPNRKYFGSGRGITWYNFVSDQYSGFHGIVIPGTLRD
                     SIFVLEGLLEQQTGLNPVEIMTDTAGTSDIIFGLFWLLGYQFSPRLADAGEAVFWRAD
                     KAANYGALDELARGCVDLSKIESHWDEMMRVAGSLKLGTIHASELIRSLLRSTRPSGL
                     AQAIMEVGRVNKTLYLLNYIDDEDYRRRILTQLNRGEGRHAVARAICYGQRGEIRKRY
                     REGQEDQLGALGLVTNAVVLWNTLYMQEALSHLRSTGEGPEDEHIARLSPLMHGHINM
                     LGHYTFTLPEDIMKGELRPLNLNLNNELPP"
     gene            4262..>4805
                     /locus_tag="RHZ76_004224"
     CDS             4262..>4805
                     /locus_tag="RHZ76_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV2814743.1"
                     /translation="MALLGYARVSTSHQKLTSQVTELNTAGVRDDRVFTDMMSGATDE
                     REGLQRLLARAEKDDIILCTKMDRLGRNTADMIRIVDTCYKKGIAIRFLENGLSTEGT
                     MGKMVIQILAAVAEAERERILERTNDGRQAAMIKGVKFGRKPHRKAELANELINKDVE
                     VKEVMEKTGISRATYFRLKRK"
BASE COUNT         1226 a         1205 c         1200 g         1174 t
ORIGIN      
        1 aaaatgaact aaataaagcc gaaaaacgtg acggtgtcac ctctggggtg tttactttgg
       61 tatctagtta ttagtaaagg catgactaaa tgtataatgg ggtagtcgtt tacgtttagg
      121 gcttcctctt tgtcgttatg gcggatgagg ggctgttacg atagttagac agtgattaca
      181 cacatttggc tgagggctac aaaatggcgg cgtcgcgaat tacacaccta ataacgtcct
      241 gtaccaaagg taagcacagc cagtgcggct ccatgccaga actgagtatc cggtctggac
      301 agacaccaga agaggcaatg tccagctggg cagcaacgat taaacggtcg caaagtgctt
      361 caccggtacc ggccctgtcg ctctatgccg gtaatcactg gtcaacggca aaagagatat
      421 tacggacaac tgagaacctg gagttgtggg tgatctcggc cgggttgggt tttttgaata
      481 gtcgtgacct cgtggacgct tatgaagcca cctttcatga tttaccgttc agtcaccgac
      541 aatggtggcg ggaattaaca aatacattcg ggaaagaaag gacggcgaaa tctattgaga
      601 cattgatggc agcaagacct ttcgatgatt atgttatcgc tgcctccccc gtttatatcg
      661 aggcgacgga ggatgatatc ctggcagggg caagcaaatt aaataatcac attgcccagt
      721 tgacggttgt aacgtcaggg gaatattctg gactacttga atcctattta attcgtagcg
      781 aatcccgtat gatgcgccaa ctatcaagca acatggtatg tcttaacatc aaacttgctc
      841 aatatattat tggaagtcgg cgctattcat gaaccgcact gatgattcca taacgcaact
      901 atctacactt tatgaacggc tcggagctgt ggctcatgac tggaatattg taaaattctg
      961 ctccctgaac actctgaaca actggatctt gatgccgcag caacatatgc aatgatgctc
     1021 cgcattgaac agtgggcagc aaacgttaag aacgcatata aggggtttga gggccaatga
     1081 tgtaaaaaac cacgctaagg aggtaattca ttgtttaaat tgagatttaa cggcctcaat
     1141 tcccccttca taatatcctc cggtagtgtg aacgtataat gtcccagcat gttgatatga
     1201 ccatgcatca gaggcgaaag ccgcgcgata tgttcatctt ctggcccttc cccagtgcta
     1261 cgcaggtgcg acaaggcttc ctgcatataa agcgtattcc acagcaccac tgcgtttgtc
     1321 accagaccca gcgcacccaa ctgatcttcc tgcccctcgc ggtaacgttt tctgatctca
     1381 ccacgctgac cgtagcaaat ggctcttgcc acggcatggc gaccttcgcc ccggttaagc
     1441 tgtgttagga tcctgcggcg atagtcctca tcatcaatat agttgagaag atacagtgtc
     1501 ttgttaactc ttccgacctc cattattgcc tgagcaagcc ctgacggtcg ggtacttctc
     1561 aatagcgatc gaatgagttc tgacgcatga attgtgccca gcttcagcga acctgcaacc
     1621 cgcatcatct catcccagtg gctttcgatt ttagaaaggt caacacaacc ccgagccagt
     1681 tcgtccaacg ccccgtaatt cgctgctttg tcagcccgcc agaacaccgc ctcaccagca
     1741 tccgccagac gaggtgaaaa ttggtatccc agtagccaga acaagccaaa gataatgtca
     1801 ctggtacctg ctgtgtccgt catgatttca accgggttta gtcccgtttg ttgctcaagt
     1861 agtccctcca gcacaaaaat ggaatcccgc agcgttccag ggatcactat gccatgaaaa
     1921 cccgaatatt ggtcagagac aaagttgtac caggtaatgc cgcgccctga accaaagtat
     1981 tttctgttgg ggccagaatt caccgttttc actggtgtga caaaacgcat accatcagcg
     2041 gaggccacct caccgccacc ccagtatcct gccaacgcta acgaggactg aaaatcaacc
     2101 agccgcgcat tggcgctgac cagcgtttct gctcggatgt agttctgttt cacccagctc
     2161 aggcgatggc gggtcagtgc agggatattg tgcttgatca gcggctcatg tccgatattg
     2221 caggcctcag ccagcatcac cgcacacagg attatatgca gatcctgcgc gcgggcacct
     2281 gactcgctga catggctgaa ttcacgggtg aaacccgttc tggcgtcaat ctcaagcaac
     2341 agttctgtca ggtcaacggg tgggagcaat tgtctgactc ggctgctcag tcgtatcagc
     2401 gccggtggct catccaattt atccaggctg ctgatggtca gcgaaggatg tttaccttca
     2461 ttgcagatgt ctacggcggt attgcggtca aaacgtgatg ccaccgtttt ccaggtttca
     2521 tccaaccgtg ctgccaattg caccgatgct ttactgccat tggtggggtg acccaatgcc
     2581 cggcacactg gaacccgctg cgcctgccat tcctccccct gaagaagttt ttggcgcgga
     2641 tcaccccaac gatcgctgtt ttccaaccag atgtcacgac ggcgtagcgc atcctgaagt
     2701 cgctccagca gacacaacga gtaacccgct cgctgtatcc gaccttcagc atcgtacacc
     2761 aggcgtttcc aagggcctga aatgatatgc tcaggtgcat cgtccaggat ccgttttttc
     2821 gagccgttta gctctgccag gtaatggatg gccgcgaggg tatgttcacc gtctggggcc
     2881 gcccggaagt gcagatcgcg cagtagtgcg ggcaaaaaac gccttacccg tccgtactgc
     2941 tccaccattt catcctgaaa gtttgtatcc tgggggcggg ccagttcatt cactttttcc
     3001 actgactctg ccagtctggc gacaggtaca ctgctgaata tggtctttcg cagcaaatca
     3061 tcaccagtat cttcatcaag taaaagtgca catgcccgcg ccagcaacaa cgccgcacgg
     3121 tcaagatcct tgagcgtcct aagtcgcttt ttctgcccgg ttttcttcgc ctccctggta
     3181 atattgagga tcagcatatc cagcacgtca acggcctcgt ccagcgccga gatttcttgc
     3241 gctttcacga acgcggtaag gatagccagc cgccgttctt ctggcattct gctgatatat
     3301 ttcaccgatg ccattccggc atagcgtgcc aggttgcgga gctgtatggc aggcagccca
     3361 gtgaaattca ggcaggaaaa ttccaggctg cgcaggcgag tgtaacgttc aagtgcttca
     3421 gtgaatgcgg ggccgctgat agtgacaggg cctcttttta ggtgctccat cactgagagt
     3481 cgctgtcctt cggggatttc aagtaaccca gccagttgtg cggcctgcca acggtttggc
     3541 agcgcggcca attttcgcca taagcggcgg gtcgcacgct cacggatttc accaatgacc
     3601 ctcgtaaggg tggatgcggc tggcaacaac actttatttt gaagcaacca cgcggtggca
     3661 aaatcgaaca tcagtccggg gcgttcatta ctgagccatg cacgagtata cagcaatcgc
     3721 ttcagtctga acgaccacgg gaaatcaccg aaatcatgat agctgtagtg ctgcctgata
     3781 agcccgtggt gctcccaacg tgtattttcc ctttgagcat agcgggagat gatctctgga
     3841 tagcggatgt taagttgcct tgcgacataa aactggacac cgggcggtat ttgcatgaga
     3901 tcgggcaaga aggttcccag aaagcgggcg gttgtgagtt gaagggcaat ccccaaccgg
     3961 ttatgcctgc ccctacgtag gttgatgaaa gtaaggtctc gctcatcaag atgaaaatac
     4021 cgtgccagtt ggatctcgtt tggctctgcg gcataacgcc cgtagttctg tgtctgtttg
     4081 tcggtcagaa aatcagcagc catcacgcat cctttcatta tttattgatc cgtattattt
     4141 tggcggtatc agatattata aattttgaga ccaatgaaaa ctgccctaaa catgagtatc
     4201 aaaaatcgtg atttatgcct tatgatacta cgcagtatca atatcgattt atgagaccaa
     4261 aatggcatta ttagggtatg caagagtatc aaccagccat cagaaactga cgagccaggt
     4321 tactgagctc aacacagcgg gagtgaggga tgatcgagtg tttactgaca tgatgtcggg
     4381 agcaactgat gagcgcgaag ggctacagcg actccttgcc agagcggaga aagatgacat
     4441 tattctctgt acaaaaatgg atcgtcttgg acgtaatacc gcagatatga ttcgcatcgt
     4501 ggatacttgc tataaaaaag gcatcgctat tcgctttctt gagaacggcc ttagtactga
     4561 aggcactatg ggaaaaatgg tcatccagat actagctgct gttgccgaag cggaacgtga
     4621 aaggatcttg gaacgaacta acgatggacg gcaggcagct atgattaagg gcgtcaaatt
     4681 cggaagaaaa cctcatagaa aggctgaatt agctaatgaa ttgatcaata aggacgtaga
     4741 agtaaaagag gtaatggaaa aaacgggtat atcacgggcg acgtatttta ggctgaaaag
     4801 aaaaa
//
LOCUS       DAPHBJ010000257         4801 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.259, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000257 DAPHBJ010000000
VERSION     DAPHBJ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.259"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..155)
                     /locus_tag="RHZ76_004225"
     CDS             complement(<1..155)
                     /locus_tag="RHZ76_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310470.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates concomitant with their translocation across the
                     cell membrane; involved in N,N'-diacetylchitobiose
                     transport; protein IIA transfers a phosphoryl group to IIB
                     which then transfers the phosphoryl group to the sugar;
                     IIC forms the translocation channel for the sugar uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HDV2814744.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNV"
     gene            complement(240..560)
                     /gene="chbB"
                     /locus_tag="RHZ76_004226"
     CDS             complement(240..560)
                     /gene="chbB"
                     /locus_tag="RHZ76_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HDV2814745.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVATIKK
                     AAAN"
     gene            complement(859..1197)
                     /gene="osmE"
                     /locus_tag="RHZ76_004227"
     CDS             complement(859..1197)
                     /gene="osmE"
                     /locus_tag="RHZ76_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HDV2814746.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1399..2226
                     /gene="nadE"
                     /locus_tag="RHZ76_004228"
     CDS             1399..2226
                     /gene="nadE"
                     /locus_tag="RHZ76_004228"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDV2814747.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALACPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2456..3343
                     /gene="cho"
                     /locus_tag="RHZ76_004229"
     CDS             2456..3343
                     /gene="cho"
                     /locus_tag="RHZ76_004229"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HDV2814748.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3303..3878)
                     /gene="ves"
                     /locus_tag="RHZ76_004230"
     CDS             complement(3303..3878)
                     /gene="ves"
                     /locus_tag="RHZ76_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HDV2814749.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESTDRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(4081..4566)
                     /gene="spy"
                     /locus_tag="RHZ76_004231"
     CDS             complement(4081..4566)
                     /gene="spy"
                     /locus_tag="RHZ76_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HDV2814750.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT         1226 a         1143 c         1139 g         1293 t
ORIGIN      
        1 aaaacgttgt taattaatac aaacatggcc cccgcaaggg ttaacggcat taagcgaata
       61 aagccatttt tgattgcatt aacgtgtggc tgctttccta ttttaactgc aaaagggagg
      121 agtacctttt caagcgatgc aataacatta ctcatagaaa aataccctta aaaaccgcaa
      181 tttaaatatt gcggtattga tttatgaaat aactctttga cgggaaaatt taaaataaat
      241 taatttgctg cggctttttt aatcgttgca accgcagcct taagcacgcc taaaccatcg
      301 actttgccat aaagcagcga gtcaattact tcaaccggtt tgttgggtaa caaacgctgg
      361 atttcgggca acatataagc aatctgcggc cctaataaca cgacatcggc attctgacct
      421 ttttcaccag ccagtgtttc cggaaatgct tcaataatga ccggaacttc atatttttct
      481 gcctgtgcgc gcatttttga taccagtaaa gaggtagaca tgcccgcaga acaaaacaga
      541 taaatgtgtt tcttttccat aaaactgccc tcatcgacga ttatctgtca gccagacact
      601 ccgcaagcct taacctgctt ccatgctctg ggtaacttgc gaaaccaaca tgactttttt
      661 tgtggctgaa aggagtatac ggtatagacc tgagcaatag tatttctttg accttataaa
      721 ttgtttctcc ttgacctgtg gttatgaccc tcttcacatt tcgggcaaat aattcgcgaa
      781 gataattaag aactcaggcc aaaaaaaacc ggcgcaatgg ccggtttccg ttgttactca
      841 agctttcaga cgaattgatt acttcgcagc ctgtggatca gtgtcgtatt cagcacaggt
      901 ctgataaccg gagttgatga catgtccggt atcatctaac gcgacaaagt aggtttctgc
      961 tttaccatca cgttgaccca ggatgtaggt ctggcaagta ccgcgagcat ggatcatgct
     1021 cacttcagac gaaggtttac ccgcaatttg tgcaacctgc gcccggctca tgcctttttt
     1081 gacgtctttc accacaggct gtacaaactg gtctttggta cgatcataag ccgtacaacc
     1141 cgccagcatg gttaataccg ccgctgcact cagaattcct gccatattct tgttcatatt
     1201 ccgtcctctt gtttatcagc gtgttagata agcctggaat acattgggcg ctttttcaag
     1261 cccgtgaacg aaacggctcc gctttcagag gattcctgta tgacgtttta accaccattc
     1321 agcccgctgt cgcttgtcgt ttcagtagca acgggttagc tttaaggaag ttttgtcttt
     1381 tctgtctgga ggggttcaat gacattgcaa caacaaataa taaaggcgct gggcgcaaaa
     1441 ccgcagatta atgctgaaga ggaaattcgt cgtagtgtcg attttctgaa aagctacctg
     1501 caaacttatc cgttcattaa atcactggtg ctcgggatca gcggcggtca ggactccacg
     1561 cttgccggaa agctgtgcca gatggcgatt aatgagctgc gcctggaaac cggcaacgaa
     1621 tcactgcaat ttattgccgt acgcctgccc tatggtgttc aggccgacga acaagattgc
     1681 caggatgcca ttgcctttat tcaaccggat cgcgtattaa ccgttaatat caagggcgcg
     1741 gtattggcca gcgaacaggc attgcgggaa gcaggcattg aactgagcga ttttgtccgt
     1801 ggcaatgaaa aagcgcgtga gcggatgaaa gcacaatata gcattgcggg tatgaccagc
     1861 ggtgtcgtgg tgggcaccga tcatgcagca gaagccatta ccggattctt cactaaatat
     1921 ggtgacggcg gtacggacat taatccgctg tatcgtctca acaaacgtca gggtaaacag
     1981 ttactggcgg cattagcttg cccggaacac ctttataaga aagcgccaac ggccgatctg
     2041 gaagatgatc gcccttctct gccagatgaa gtggcactcg gcgtgaccta tgacaatatc
     2101 gacgactatc tggaagggaa aaacgtacct caacaggtcg ccagaacaat agagaactgg
     2161 tatctgaaaa ccgaacataa acgccgtccg ccaattaccg ttttcgatga tttctggaaa
     2221 aagtaataat ttgcacatat tggattgtgc gaaaaagagt aatttgttca cgccggatgc
     2281 ggcgtgaacg ccttattcga cctataaaac tatgcaaatt caatacattg caggagtcga
     2341 ataggcctga caggcgtagc acgtcagacg gtgtaacctt tgtcatcgac ccgcctcttt
     2401 tttaatcgct tcccgcctgt tacactggat agataaccag cattcggagt caacagtggt
     2461 acggcgttta acttctccgc ggctcgaatt tgaagctgcg gcaatttatg aatatcccga
     2521 acatttacgt tcattcctta atgacttacc cacccgacca ggggtgtatc tgtttcatgg
     2581 tgaaagtgac accatgccgc tctatatcgg caaaagcgtt aacatccgca gccgcgtcct
     2641 ttctcattta cgtaccccgg atgaagccgc catgctacgg caatcccgac ggatcagctg
     2701 gatatgtacc gccggtgaaa tcggcgctct gctccttgaa gcgcgattaa tcaaagaaca
     2761 acagccgctg tttaataaac ggttgcgccg caatcgccag ctctgtgccc tgcaattaaa
     2821 tgaaaagcgc gtcgatgtgg tgtatgccaa agaggtggat ttttcacgag cccccaacct
     2881 gtttggcctg tttgccaata ggcgcgcagc tttgcaagca ttgcagacca tcgctgatga
     2941 acaaaaactt tgttatggcc tgttgggact ggaaccgtta agtcgcggtc gtgcatgttt
     3001 tcgttcagcg ctaaaacgtt gcgctggggc gtgttgcggt aaagagagcc atgaggaaca
     3061 tgcgctacgc ttgcgccagt ctctggagcg tttgcgggtg gtgtgttggc cttggcaagg
     3121 ggcggtggcg ctgaaagaac agcacccgga aatgactcaa tatcatatta ttcaaaactg
     3181 gctgtggctg ggggcggtta attcgctgga agaagcgaca acgttaattc ggacacccgc
     3241 cgggtttgat cacgacggtt ataaaattct ttgtaagccg ctgctttccg gtaactatga
     3301 aattactgaa cttgatccgg cgaatgacca gcgagccagt tgatttcact gaacaacagt
     3361 ttaccctgcg gttgtaaaag ccgcagggtg tgcctgccat cgaaccagca cgcgccctgg
     3421 tcggtagtga gcagtttgtc gcccagttgc cacgccccgt taatcacaaa caccaccccg
     3481 ccgcgtgagc caaaggtagt aaacgtacgt tcggcaattc tgactttcgc cttacagaca
     3541 tccaggcgtg tcatgatgtt gaaatccatc gacatctgcc cttccgtcag tttcgctttt
     3601 accacctggt ccgccgcaaa ggcaaaaggt tgtaacggtt ttaaagtatg gttaaagcgg
     3661 tctgtgcttt caagaaacat ctcgccgcct tcaagcaacg tcactatcct ttccatgcct
     3721 ggaaacaaag aaaactcccc attcgccgcg atggatgcaa tgctggcacg ccagtaaaaa
     3781 tcacgttttg ccggtgggaa cgtgcaaatt tcgcgcgttt caccggcagc gtttcgccac
     3841 agattcaccg acattttacg catatcaaag tattccatac tcgctcccgg cccgttcttt
     3901 tcattgttat cgttcaggta accgtggatg gttatcggca actcgggtga tttacgttag
     3961 tggcgatcag gcttttttta caactgtttg agaagagaaa agaaaaccgc cgatcctgtc
     4021 caccgcatta ctgcaaggta gtggacaaga ccggcggtct taagtttttt ggctgaaaga
     4081 ttattcagca gttgcaggca ttttaccttt tgccgctgga cgttctgtca gacgcttctc
     4141 aaaattagca ttaaattgct ttttctgttc cggcgtcagg atgttgtaaa ttttgttctg
     4201 ggtttccatg tgcgccagca tgttagcttt gcgctgttct tccatttttg cgatctgcgc
     4261 ttcagctttt gctttatcga aggtatcgct ggcaatgatg tcatgcattg cgcggcgttc
     4321 ttccagcggc ggacgtttca tctggtcacg ctggcctttc atgatttcgc ggatctgctg
     4381 tttttgcgcg tcggtcaggt tcaggtcttt gaacatcatg tcctgatgcg gaccgaactt
     4441 gcctttatgg tgcatcatcg gcttcgcgtc agccggtgct gcggtagtgg tgtctgcggc
     4501 atgggccagg ttagccgcgc caagagccag ggtagaggca acaaacagtg cagttaattt
     4561 acgcatattc tatatccttc ctttcagtta tttattacgg ctttcttaag tagcgtgccg
     4621 tgttgacgag attaacttta ctgactttag cgtcaattaa tcagagcaac ggtaaaacaa
     4681 tgaaagtgta aaaaacactt ttgcgccaat tatggagaaa aaaagaaaat gtgatggaga
     4741 gtgatgagag aatattacaa caccatgatt ttgcagagat tatgaagaac tataccggat
     4801 g
//
LOCUS       DAPHBJ010000258         4798 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.260, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000258 DAPHBJ010000000
VERSION     DAPHBJ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.260"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..645
                     /gene="fimC"
                     /locus_tag="RHZ76_004232"
     CDS             1..645
                     /gene="fimC"
                     /locus_tag="RHZ76_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDV2814751.1"
                     /translation="MSASAHAAGGIALGATRIIYPADAKQTAVWIRNSHTNERFLVNS
                     WIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAADRESLFWMNVKTIPSVD
                     KNALNGRNVLQLAILSRMKLFLRLIQLQELPAEAPDTLKFSRSGNYINVHNPSPFYVT
                     LVNLQVGNQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDYGSVTPVREVNLN"
     gene            676..3285
                     /locus_tag="RHZ76_004233"
     CDS             676..3285
                     /locus_tag="RHZ76_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HDV2814752.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERISLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGIRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGTVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGRKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            3298..4305
                     /gene="fimH"
                     /locus_tag="RHZ76_004234"
     CDS             3298..4305
                     /gene="fimH"
                     /locus_tag="RHZ76_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HDV2814753.1"
                     /translation="MKIICRLLLAMACLWLTNISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLHD
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            4316..>4798
                     /gene="sfmF"
                     /locus_tag="RHZ76_004235"
     CDS             4316..>4798
                     /gene="sfmF"
                     /locus_tag="RHZ76_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HDV2814754.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     K"
BASE COUNT         1239 a         1062 c         1186 g         1311 t
ORIGIN      
        1 atttcggcca gcgcccatgc tgccggaggg atcgcattag gtgccacgcg tattatttat
       61 cccgctgatg ctaaacagac tgcggtatgg attagaaata gccataccaa tgagcgcttt
      121 ctggtcaatt cgtggattga aaacagcagc ggtgtaaaag aaaagtcatt catcattaca
      181 ccgccactgt ttgttagtga acccaaaagc gaaaatacct tgcgtattat ttacaccggt
      241 ccaccgctgg cagcagatcg tgagtctctg ttctggatga atgttaagac gatcccttcg
      301 gtagataaaa atgcattgaa cggcaggaat gttttgcaac tggcgatttt atcgcgcatg
      361 aaattatttc tccgtctaat tcaattacaa gaattacccg cagaagcacc ggacacactc
      421 aagttttcgc gatccggtaa ctatatcaat gttcataatc cctcaccttt ttatgtcact
      481 ctggttaact tacaagtggg caaccaaaag ttggggaatg ctatggctgc acccagagtt
      541 aattcacaaa ttcccttacc ctcaggagtg cagggaaagc tgaaatttca gaccgttaat
      601 gattatggtt cagtaacccc ggtcagagaa gtgaacttaa actaaccgaa tcatctgaca
      661 atatcagagc taattatgaa aatacccact actacggata ttccgcagag gtatacctgg
      721 tgtctggccg gaatttgtta ttcatctctt gccattttac cctccttttt aagctatgcg
      781 gaaagttatt tcaacccggc atttttatta gagaatggca catccgttgc tgatttatcg
      841 cgctttgaga gaggtaatca tcaacctgcg ggcgtgtatc gggtagatct ctggcgtaat
      901 gatgagttca ttggttcaca ggatatcgta tttgaatcga caacagaaaa tacaggtgat
      961 aaatcaggtg ggttaatgcc ctgttttaac caggtactcc ttgaacgaat tagccttaat
     1021 agcagtgcat ttcccgagtt agcccagcag caaaacaata aatgcatcaa tttactgaaa
     1081 gctgtacctg atgccacaat taactttgat tttgcagcga tgcgcctgaa catcactatt
     1141 cctcagatag cgttgttgag tagcgctcac ggttacattc cgcctgaaga gtgggatgaa
     1201 ggtattcctg ctttactcct gaattataat ttcaccggta acagaggtaa tggtaacgat
     1261 agctattttt ttagtgagct cagcgggatt aatattggcc cgtggcgttt acgcaacaat
     1321 ggttcctgga actattttcg cggaaatgga tatcattcag aacagtggaa taatattggc
     1381 acctgggtac agcgcgccat tattccgctg aaaagtgaac tggtaatggg agacggcaat
     1441 acaggaagtg atattttcga tggtgttgga tttcgtggta tacggcttta ctcttccgat
     1501 aatatgtatc ctgatagcca gcaagggttt gccccaacag tacgtgggat tgcccgtacg
     1561 gcggcccagc taacgattcg gcaaaatggt tttattatct atcaaagcta tgtttccccc
     1621 ggcgcttttg aaattacaga tttgcacccg acatcttcaa atggcgatct ggacgtcacc
     1681 atcgacgagc gcgatggtaa tcagcagaat tacacaattc cgtattcaac agtgccaatt
     1741 ttacaacgcg aagggcgttt caaatttgac ctgacggcgg gcgattttcg tagcggtaat
     1801 agtcagcaat catcgccttt cttttttcag ggtacggcac tcggcggttt accacaggaa
     1861 tttactgcct acggcgggac gcaattatct gccaattaca ccgccttttt attagggctg
     1921 gggcgcaacc tcgggaactg gggcacagtg tcgctggatg tgacccatgc gcgcagtcag
     1981 ttagccgacg acagtcgtca tgagggggat tccattcgct tcctctatgc gaaatcgatg
     2041 aacaccttcg gcaccaattt tcagttaatg ggttaccgct attcgacaca aggtttttat
     2101 acccttgatg atgttgcgta tcgtcgaatg gaggggtacg aatatgatta cgattacgac
     2161 ggtgagcatc gcgatgaacc gataatcgtg aattaccaca atttacgctt tagccgtaaa
     2221 gaccgtttgc agttaaatat ttcacaatca cttaatgact ttggctcgct ttatatctct
     2281 ggtacccatc aaaaatactg gaatacttcg gattcagata cctggtatca ggtggggtat
     2341 accagcagct gggttggcat cagttattca ctctcatttt cgtggaatga atctgtaggg
     2401 atccccgata acgaacgtat tgtcggactt aatgtttcag tgcctttcaa tgttctgacc
     2461 aaacgtcgct acacccggga aaatgcgctc gaccgcgctt atgcctcctt taacgccaac
     2521 cgtaacagca acgggcaaaa tagctggctg gcaggtgtag gtgggacctt actggaaggc
     2581 cacaacctga gttatcacgt aagccagggt gatacctcga ataatgggta tacgggcagc
     2641 gccacggcaa actggcaggc cgcttacggt acgctggggg tcgggtataa ctacgaccgc
     2701 gatcaacatg acgttaactg gcagctgtct ggcggtgtgg tcgggcatga aaatggcata
     2761 acgctgagcc agcctttagg ggataccaat gttttgatta aagcgcctgg cgcaggcggt
     2821 gtacgcattg aaaatcaaac tggcatttta accgactggc gcggctatgc ggtgatgccg
     2881 tatgccacgg tttatcggta taaccgtatc gcgcttgata ccaatacgat ggggaattcc
     2941 atcgatgttg aaaaaaatat tagcagcgtt gtgccgacgc aaggcgcgtt ggttcgtgcc
     3001 aattttgata cccgcatagg cgtgcgggcg ctcattaccg ttacccaggg cagaaaaccg
     3061 gtgccgtttg gatcactggt acgggaaaac agtaccggaa taaccagtat ggtgggtgat
     3121 gacgggcaag tttatttaag tggtgcgcca ttgtctggtg aattactggt tcagtgggga
     3181 gacggcgcga actcacgctg cattgcgcac tatgtattgc cgaagcaaag cttacagcaa
     3241 gccgtcacag ttatttcggc agtttgcaca catcctggct cataaaggaa attatcaatg
     3301 aagataatct gcagattatt attggcgatg gcatgtttgt ggttaacaaa catatcctgg
     3361 gctactgttt gtgcaaatag tactggcgta gcagaagatg aacactatga tctctcaaat
     3421 gtctttaata gcaccaataa ccagccaggg cagattgttg ttttaccgga aaaatccggc
     3481 tgggtaggtg tgtcagcaat ttgtccaccc ggcacgctgg tgaattatac ataccgtagt
     3541 tatgtcacca actttattgt tcaggaaact atcgataatt ataaatatat gcaattacat
     3601 gattatctat taggtgcgat gagtctggtt gatagtgtta tggatattca gttccccccg
     3661 caaaattata ttcggatggg aacagatcct aacgtttcgc aaaaccttcc attcggggtg
     3721 atggattctc gtttaatatt tcgtttaaag gttattcgtc cctttattaa catggtggag
     3781 atccccagac aggtgatgtt taccgtgtat gtgacatcaa cgccttacga tccgttggtt
     3841 acacctgttt ataccattag ttttggtggc cgggttgaag taccgcaaaa ctgcgaatta
     3901 aatgccgggc agattgttga atttgatttt ggtgatatcg gcgcatcgtt atttagtgcg
     3961 gcagggccgg gtaatcgacc tgctggtgtc atgccgcaaa ccaagagcat tgcggtcaaa
     4021 tgtacgaatg ttgctgcgca ggcttattta acaatgcgtc tggaagccag tgccgtttct
     4081 ggtcaggcga tggtgtcgga caatcaggat ttaggtttta ttgtcgccga tcagaacgat
     4141 acgccgatta cgcctaacga tctcaatagc gttattcctt tccgtctgga tgcagctgcg
     4201 gcagccaatg tcacacttcg cgcctggcct atcagtatta ccggtcaaaa accgaccgag
     4261 gggccgttta gcgcgctggg gtatttacgc gtcgattatc aatgaggtac ggagaatgag
     4321 aagagtactc tttagctgtt tctgcgggtt actgtggagt tccagtggat gggcagctga
     4381 ccctttagga acgattaata tcaatttgca cggtaacgtt gttgatttct cctgtaccgt
     4441 aaacacagcg gatattgata agacggtaga tttaggcaga tggcctacga cacaattact
     4501 gaacgctggc gataccacgg cactcgtccc ttttagcctg cggctggagg gatgtcctcc
     4561 gggttcagtt gcgattttat ttacgggaac gccggcatcc gataccaacc tgctggctct
     4621 ggatgatccc gcaatggcac aaaccgtcgc catcgaatta cgtaatagcg atcgctcccg
     4681 gctcgcactg ggggaggcga gcccgactga ggaagtagat gcaaatggca atgtcacact
     4741 aaactttttt gccaattatc gagcgttagc cagcggtgtt cggccaggtg tggcgaaa
//
LOCUS       DAPHBJ010000259         4745 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.261, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000259 DAPHBJ010000000
VERSION     DAPHBJ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.261"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..636
                     /gene="hprR"
                     /locus_tag="RHZ76_004236"
     CDS             <1..636
                     /gene="hprR"
                     /locus_tag="RHZ76_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HDV2814755.1"
                     /translation="QEWVTQGLSEAGYVIDAVSDGRDGLYLALKEDYALIILDIMLPG
                     MDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPFSFSELLARVRAQ
                     LRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLASRAGEIIPRTVI
                     ASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSFVAVKK"
     gene            636..1994
                     /gene="hprS"
                     /locus_tag="RHZ76_004237"
     CDS             636..1994
                     /gene="hprS"
                     /locus_tag="RHZ76_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HDV2814756.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDGMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(2102..2953)
                     /gene="hchA"
                     /locus_tag="RHZ76_004238"
     CDS             complement(2102..2953)
                     /gene="hchA"
                     /locus_tag="RHZ76_004238"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HDV2814757.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(3545..4732)
                     /gene="ompC"
                     /locus_tag="RHZ76_004239"
     CDS             complement(3545..4732)
                     /gene="ompC"
                     /locus_tag="RHZ76_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDV2814758.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEVYNKDGNKLDLYGKVAGLHYF
                     SDDAGSDGDKSYARIGFKGETQIADQFTGYGQWEFNIGANGTESDKGNTATRLAFAGL
                     GFGQNGTFDYGRNYGVVYDVEAWTDMLPEFGGDTYAGADNFMNGRANGVATYRNNGFF
                     GQVDGLNFALQYQSNNENGGPFGQEGSGKGKGRDIAQENGDGFGMSTSYDFDFGLSLG
                     AAYSNSDRTDNQVHKGTHNTRYGDRFDATAGGETAEAWTVGAKYDANNVYLAAMYAET
                     RNMTGYGDADAIANKTQNFEVVAQYQFDFGLRPSIAYLQSKGKYLGGVNSDNFGSQGN
                     HHYTNKDLVKYVDIGMAYYFNKNMSTYVDYKINLLDNDDDFYKENGIATDDIVAVGLV
                     YQF"
BASE COUNT         1321 a          982 c         1110 g         1332 t
ORIGIN      
        1 caggagtggg taacgcaggg gctttccgaa gcgggttatg tcattgatgc cgtttctgat
       61 ggcagagatg ggctttatct tgcgctgaag gaagattatg cattgatcat tctggatatt
      121 atgcttccgg gtatggatgg ctggcagatc ttacaaacgt taagaacggc aaagcaaacc
      181 cctgttattt gccttactgc aagggattct gtcgatgaca gagtcagagg actggacagt
      241 ggagcaaatg attatctggt aaaacctttt tcattttctg agttgctggc aagggttcgg
      301 gcacaattaa ggcaacatca cgctttgaat tcaacattag aaatcagcgg cttaaaaatg
      361 gactctgtta gtcaaagtgt gagcagggac aatatcagta ttacactgac gcgcaaggag
      421 tttcagttac tttggctact ggcctccaga gctggcgaaa ttatacccag aacggttatt
      481 gcgagtgaaa tttggggaat caactttgat agtgatacca atacggtgga tgtcgccatt
      541 cgcaggctcc gcgcaaaagt tgatgatcct tttcctgaaa agctaattgc cacaatccgt
      601 gggatgggat attcgttcgt agcggtaaaa aaataatgaa aagattgtct ataaccgtcc
      661 gtttaacctt gctttttata ttgctactgt ctgttgctgg tgctggaatt gtctggactc
      721 tctataatgg cctggcaagt gagttgaaat ggcgcgatga tacaacactc attaaccgga
      781 cagcacagat caagcagttg ttaattgatg gggtaaatcc agatacgtta cctgtgtact
      841 ttaaccggat gatggatgtt agtcaggata tcttgattat tcatggtgat agcatcaata
      901 aaattgttaa ccggacaaat gtcagtgatg gcatgttaaa taacatacct gctagtgaga
      961 caatcagcgc agctggcatt tacagaagca ttattaatga tacagagata gatgctttac
     1021 gaattaatat tgatgaagtt tcgccatcat taacggttac tgtggctaaa ttggcttcag
     1081 ccagacataa catgcttgaa cagtataaaa tcaatagcat tataatttgc attgtcgcca
     1141 ttatactgtg ctcagtatta agtccgctgt taatccgaac ggggttacga gagatcaaaa
     1201 agttgagtgg tgtaacggaa gcgctgaatt ataacgatag ccgggagcct gttgaggtta
     1261 gcgcattacc gagagaacta aaacctcttg ggcaggcgtt gaataaaatg catcatgctt
     1321 tagtcaaaga ttttgagcgt ctaagtcaat ttgctgacga tctcgctcat gaacttagaa
     1381 cgccaattaa tgcattactg ggtcagaatc aggttacgct cagtcaaacc agaagtatcg
     1441 ctgaatatca aaaaacaatt gccggtaaca ttgaagagct ggaaaatatt tcgcggttaa
     1501 cagagaacat actgtttctt gccagggcag ataaaaacaa tgttttggtg aaactggact
     1561 cgctttctct caataaggaa gtcgaaaatt tgttggacta tcttgaatat ctttcagacg
     1621 agaaagagat ttgctttaag gtcgagtgca atcagcaaat ctttgcggat aaaattttac
     1681 tgcaacgaat gttatcgaat cttattgtta atgccattag atattcacca gaaaaatcgc
     1741 gtattcatat aaccagtttt cttgatacca acggatctct taatattgat atcgccagcc
     1801 ctggaacgaa aattaatgag cctgaaaaac tcttccgtag attttggcgg ggagataatt
     1861 cgcgtcattc cgtaggtcag ggactaggcc tttctttagt caaagcgatt gccgaattac
     1921 atgggggaag tgctacgtat cactatctca ataagcataa tgtgttccgg attacgttgc
     1981 cgcaaagaaa ttaacatagg gcttcagtac gccataaatt aaccgtgaaa aaacgcagcc
     2041 acgtcgtgag tactaacgcg gccgcgattg attatgcgct tacattcaaa cgtaacaggg
     2101 attaacccgc gtaagctgcc agcatttcct gcgccgccag tttacccaac gcattcgctg
     2161 caaaaggact gtcgccggtg agaagtttac ggtccttatg tactcgcccg gtgatgtcgt
     2221 cattaatgat attcatgccc attttcttca gttcttcgcc gaagtaccag gtgagatgac
     2281 ccggcatata gccaatctct ggcgtttgtt tgtctgcggc gtctgggaat gcgcaaatgg
     2341 aataaccatt cagtgggtta tcgccgtggc gaagcgccag aaaagccgcc gggccgtggc
     2401 aaagggagat aacaaaacgg tcatttttga ttgcccactg taaagcggcg gctacgtcct
     2461 ggctttcagg taaaccaata agtgcgccat gaccaccagg aacaaagatt gctgcatatt
     2521 cgctatcagc gttgaggctg gcgacaacat ccgcgagttt cttcggattg cggaacaacg
     2581 atttatgctg ctcaaagaat ggcattactt tttcatcttt gtgcggcata gcccagtatt
     2641 caaacttggt catcagaccg gaaatggtcg ccacttcgaa ttcgaaacct gcagcatgga
     2701 gatgatacaa cggcagcaac gtttcaatcg gatggttacc ggtcgagaac agctttccgt
     2761 tatcggtcgg caaataacgt tcgtccgcgg cgatcaccag aattttatgt ttaccgcgat
     2821 acggttttgg atagtccacg ccatcaagat cagagacagg actggtatat tggctaagcg
     2881 aatattctga agggaagaat gcattatctt cagcaatatc gacctgcgga tttttacttg
     2941 tttgaacagt catagtgtat tccttattgt tgcttattta gggtagtcac tatatttgcg
     3001 actgagctgg tcgctatttt taaccaggct agaaaattga gtcaatatat cgaaagcggt
     3061 agtcggcggg tgtaagtatg taatgatgat gctgcactgg tgttgaatat tattcacgcc
     3121 aaattttcct tattgagcaa cgtcgtcgca agtggcctca atgattttag accattatca
     3181 gaggtacggg gcgacgggga gagatttagt gctgtggaat attgtggtgc tgatgaatat
     3241 ttatatcccg gattggtaga gaaaaagctt atttcatgca ctatttttgc aaatatttct
     3301 atatgggagg cggttttgtt gcagattaat tcataaagaa atgcatttgg gtcttggctg
     3361 gaagagtcgc tggtagatgt tattgtgcca gcaggggcag ggtaaggtgc tgtatcgata
     3421 tgctttggca attgcgcgtg ccgcagaggg agataaaatg ccaataaaaa agcctgagtt
     3481 cgatgaggga cgaactcagg ctagtgttaa gttttacagt aacacgccgc ggcgcgttgg
     3541 ctgattagaa ctggtagact aagcccacag ccacgatatc atcggtagcg atgccgtttt
     3601 ctttgtagaa gtcgtcgtcg ttgtccagca ggttgatttt gtaatcaaca taagtggaca
     3661 tgtttttgtt gaagtagtag gccataccaa tatcaacata tttaaccaga tctttgttgg
     3721 tgtaatgatg attaccctgg ctgccgaagt tatcactatt tacaccgcct aaatacttac
     3781 ctttagattg caggtaagcg atggacgggc gcagaccgaa gtcgaactga tactgagcta
     3841 caacttcaaa gttttgggtt ttgttagcga tggcatcagc atcaccgtaa ccagtcatgt
     3901 tacgggtttc agcatacata gctgctaagt aaacgttatt agcgtcgtat ttagcaccaa
     3961 cggtccatgc ttctgctgtt tcaccgccag cagttgcatc gaagcgatct ccataacgag
     4021 tgttatgagt acctttgtga acctgattgt cggtacggtc agaattagaa tatgcagccc
     4081 ccagacttaa accgaagtcg aagtcatagg aagtagacat accgaaacca tcaccatttt
     4141 cttgagcaat gtcacgaccc ttacccttac cagaaccttc ctgaccgaat ggaccaccgt
     4201 tttcgttatt actttgatat tgcagagcaa agttcaggcc atcgacctga ccaaagaagc
     4261 cattgttacg gtaggttgca acaccattag cacgaccgtt catgaagttg tcagcgccag
     4321 cataggtatc gccaccaaat tctggcagca tatcggtcca tgcttctacg tcatatacga
     4381 caccgtagtt acgaccatag tcgaaagtac cattctgacc aaagcctaaa cctgcaaatg
     4441 ccagacgcgt tgcagtatta cctttgtcgc tttcagtacc gtttgcgcca atgttaaatt
     4501 cccactgacc gtaaccagtg aattgatcag cgatctgagt ttcacctttg aaaccgatac
     4561 gggcatatga cttgtcgcca tcgctgccag catcatcaga gaagtagtgc aggcccgcta
     4621 cttttccgta caaatccagt ttattaccat ctttattata gacttcagcc gcatttgctg
     4681 cgccagcaac taataacgcc gggaccagca ttgccagaac ttttcttttc attatgtatt
     4741 ccctt
//
LOCUS       DAPHBJ010000260         4726 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.262, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000260 DAPHBJ010000000
VERSION     DAPHBJ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.262"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            100..588
                     /gene="eco"
                     /locus_tag="RHZ76_004240"
     CDS             100..588
                     /gene="eco"
                     /locus_tag="RHZ76_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HDV2814759.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(1079..2725)
                     /gene="mqo"
                     /locus_tag="RHZ76_004241"
     CDS             complement(1079..2725)
                     /gene="mqo"
                     /locus_tag="RHZ76_004241"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDV2814760.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPKPVQKEVADIAL"
     gene            complement(2943..4586)
                     /gene="yojI"
                     /locus_tag="RHZ76_004242"
     CDS             complement(2943..4586)
                     /gene="yojI"
                     /locus_tag="RHZ76_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HDV2814761.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1155 a         1312 c         1165 g         1094 t
ORIGIN      
        1 ttaagttatt cataaagcaa attaataaat ctgatgaata tgttaacctt cagcgacatc
       61 atcggtgaaa acctataaat gaagaaggaa agcaaaaaaa tgaagaccat tctacctgca
      121 gtattgtttg ccgctttcgc taccacttcc gcctgggcgg cagaaagcgt ccagccactg
      181 gaaaaaatcg cgccttatcc acaagctgaa aaagggatga agcgtcaggt gattcagtta
      241 accccgcaag aagatgaatc taccctgaaa gtagaactgt taatcggtca gacgctggaa
      301 gtcgattgca atttgcatcg tctcggcggg aagctggaaa acaaaacgct ggaaggctgg
      361 ggctatgatt attatgtctt tgataaagtc agttccccgg tttcgacgat gatggcctgc
      421 ccggatggca agaaagagaa gaaatttgtc accgcgtatc tgggcgatgc tggaatgctg
      481 cgttacaaca gcaagctgcc gatcgtggtg tatacgccag acaatgtaga tgtgaagtac
      541 cgcgtctgga aggcggaaga gaaaattgac aacgcggtag ttcgctaaac tgccgtgaag
      601 tgcggcaccc cgtaggtcag acaaggcggt cacgccgcat ccgacatcca acgcccgagc
      661 cggttgcctg atgcgacgct ggcgcgtctt atcaggccta caccgctgtg aagtgctcca
      721 ccccgtaggt cggataaggc ggttacgccg catccgacat ctaacgccca agccggttgc
      781 ctgatgcgac gctggcgcgt cttatcaggc ctacaccgct gtgaagtgct ccaccccgta
      841 ggttggataa ggcggttacg ccgcatccga catctaacgc ccaagccggt tgcctgatgc
      901 gacgctggcg cgtcttatca ggcctacacc gctgtgaagt gctgcacccc gtaggttgga
      961 taaggcggtt acgccgcatc cgacatctaa cgcccgagcc ggttgcctga taagacgctg
     1021 gcgcgtctta tcagcatacg ccacatcccc ggcataccat gccggatgtg gcacatcatt
     1081 acaacgcaat atccgccact tctttttgaa cgggtttcgg tttcaactgc ggtttcggcg
     1141 tactatctgc tgcctgcggc ttgtcgtatt tcagccccag cacttcgctg gtgtactgca
     1201 actcgcgttc tgttgccgct acatcaccgt tcagcttgcg tccataagac ggaacgatcg
     1261 ctttcaacgt agcctgccat tgcgggctgg aaacgcgatc gccaaatact ttttccagca
     1321 gatccaacat aatcggcgcg gcggttgacg cccctggcga tgcccccagg agcgcggcaa
     1381 tggtcccttg ctggtcactg acgacttcag tacccagacg cagtacgcca cctttctcgg
     1441 catcacgctt gataatctgc acgcgctgcc ccgcttgcca caaacgccag tcctcttttt
     1501 tcgcctgcgg atagtactct ttcaacgctt caaaacgatc ctcttcactc aacatcacct
     1561 gactcaccag atatttcacc agatcgaaat tatccagccc gacgtgcatc atcggcatca
     1621 cgttagaggt ggtggtggaa ctcatcagat cccacaatga accgtttttg aggaatttgg
     1681 ttgagaaggt ggcaaatggc ccaaacagca ctacgcgttt accgtccaga acgcgggtat
     1741 cgatatgcgg aaccgacatc ggtggtgcgc caacggatgc tttaccgtaa accttcgcca
     1801 gatggtgatt aaccacgtcc gggttttccg aaacaaggaa ctgtccgccc accgggaaac
     1861 cggcgtagtc tttcgcttcc ggaatccccg attcctgtaa cagcttcagc gccgcaccgc
     1921 ccgcgccgat aaagacaaat ttggcacgaa tgttctgtgc agtgccattt ttcagatcgg
     1981 caacggtaac ggtccaggtg ttatcgtcat tacgctttag ggcgcggact tcgctgctga
     2041 gttgcagcga gaagttagat ttcttctgca aggaagcaat taactggcgg gtgatctcgc
     2101 cgtagttcac atcggtacca atttccgtac gcgtggctgc cactttctgt tgcggatcgc
     2161 gcccttccat cactaacggt gcccactctt tgatctgcgc gtgatcttca gagtaacgca
     2221 taccgcgaaa cagcgagctt tgttgcaacg cggcgtaacg ggcgcgcagg aaattaacgt
     2281 tatcctcgcc ccagacaaag ctcatatgcg gaacggtatt gataaatgaa cgcggagtac
     2341 gcagcacgcc gcgctcaacc tggtgcgccc agaactggcg ggaaatctga aatgcttcgt
     2401 taatggcgac tgctttttca atactgatgc tgccatcggc gttttgcggg gtgtagttca
     2461 gttccatcag tgcagaatgc ccggttccgg cgttattcca gccgttcgaa ctctcctgcg
     2521 cgacaccctc caggcgctcc accatggtca tcgaccattc aggctccagc tcgcgtaaat
     2581 aggtccccaa cgtggcgctc ataatgccgc cgccaatcaa cagtacatca gtttcctgct
     2641 cctcggacgc tttcgctttt gccgccatcg aaacggcatt aagccccacg gccatcgaga
     2701 agagcatggc agtcactttt ttcatcttgt taatgcctta cttttagtcg ctttattgca
     2761 ggtgagattt gcgcggcatc aacggtaaca cttaagtaac aatatttaaa taatgtttat
     2821 aaattatatt ctaattttag aaacattata atttcgttga ttaattatag ggttattaga
     2881 aatgaaggga tttttagcat attgacctga cggcagcagt ccgccaggtc agcgtcgtaa
     2941 tattatgccg tccgggcaac ggcatcacgc gaagcagcat cgcgctcttc gcccgtcagc
     3001 tcgctaagtt gcccgttgcg catttccagc aggcggtcgg cgtggataaa gtaatgatca
     3061 tcatgactga tggcgaaaat agttttaccc atctcctgca tcagcggcag caacacctga
     3121 taaaactcac gacggaagtg tggatcctga tccgccgccc attcatccag caggataata
     3181 tcgcgttctt ctgccagcgc cagcaacagc gccacgcgtt ttttctgccc ttttgataac
     3241 ttcaggttaa caatacgccc gttgcttaac tcaagcttat gagccatttt cagctgcgcc
     3301 agccacttct caaccagttg cgggttagcg ggtttaccct ccggccccag cagttgatca
     3361 aacagccaga catcggtaaa cactgccgaa aacagtttgc gataatcttc cggttgttcg
     3421 ccgctgacag gtttgccatc cagcaagatt tcgccgcttt gtggctgata caagcccgtc
     3481 aacaacatcg ccagcgtcga ttttccgcta ccgttgccgc caatcagaaa cagcagctcg
     3541 ccacgtttga tggtgagatt aatcggacca acggaaaacg cgttatcctg ataagcaaac
     3601 gtcacgttac gcagctccag cgtttgccag ttgggaaacg cctgcgggcg cggaaactct
     3661 gctttgaaag gcgcgagcgc gaatttgttc agcttgttaa acgccacctg cgccgtcagc
     3721 agcgtcggca atgcgccaac cgccgaaagc agcggcgtac gcaggaataa aagcgtcaac
     3781 gaataggtcg cggcaacgtt ggtatcagcc caaccgaggc tgttcgccat ccagaacacc
     3841 aggccgattg cgcccagcat catgatgttt gaccagttca cggcactaag atggaaggtg
     3901 tctgcgcgaa taatatgatg gcgatactct tgcgcatcag gaatgtagag gttgttaaac
     3961 acatactcgg cgcgttcccg gttcagagtc agctctttgc gcccttcaag tacagtttga
     4021 aaatccgtgt acagcttgtc ttcggtttca cgcagggtcg ccatatgttt gtacacccgc
     4081 gccaccagca caaaaccgcc ccagatggtg atcgccatcc agatagccgt taccagcaac
     4141 attttgcccg acagcatcca cagatacgcc gctgaaccga tagtgagaat gatcccctgc
     4201 accagttccg gcagacgcac aaaagcaatg gtgatattgc gcacatcgct ggttaacccc
     4261 gccagcaacg aggcgctacc gagttgttca atgcgctcga cgtgagtatc cagaatccgc
     4321 ttgataaatt cactacgcag tcggtaaacg aagtgatgcc ccaaagtggt gagcgccagt
     4381 tgcgatccga gagtgactgc catcaacagc agcaataatc ccagaaactc cggcaacacc
     4441 agcagactgg tatccgccgt ttcgataagg cgctgattga taaaagcaat taagccaatg
     4501 cctaatgccg cactggcgag gcttagcgcc atcacactga taaatggcca gcgatactgc
     4561 cgccagacaa gtacaagaag ttccatgcag aaaacccgga caatgaatta cagcccgcag
     4621 tttaaacatc ttcgcgcgca cagcaataat aattcttatt tttattcttt tttacctgcc
     4681 tgacggaatg tcaggttgta gcggcagtcg atggtgagtg gatgaa
//
LOCUS       DAPHBJ010000261         4706 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.263, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000261 DAPHBJ010000000
VERSION     DAPHBJ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.263"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            109..1071
                     /gene="tauA"
                     /locus_tag="RHZ76_004243"
     CDS             109..1071
                     /gene="tauA"
                     /locus_tag="RHZ76_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV2814762.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1084..1851
                     /gene="tauB"
                     /locus_tag="RHZ76_004244"
     CDS             1084..1851
                     /gene="tauB"
                     /locus_tag="RHZ76_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HDV2814763.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1848..2675
                     /gene="tauC"
                     /locus_tag="RHZ76_004245"
     CDS             1848..2675
                     /gene="tauC"
                     /locus_tag="RHZ76_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HDV2814764.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2672..3523
                     /gene="tauD"
                     /locus_tag="RHZ76_004246"
     CDS             2672..3523
                     /gene="tauD"
                     /locus_tag="RHZ76_004246"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HDV2814765.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3629..4603)
                     /gene="hemB"
                     /locus_tag="RHZ76_004247"
     CDS             complement(3629..4603)
                     /gene="hemB"
                     /locus_tag="RHZ76_004247"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDV2814766.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1046 a         1221 c         1376 g         1063 t
ORIGIN      
        1 gtttttttgc taagaataaa atcatctgtg cgataacgac taattctttt aatgaatgtt
       61 tttattcctg aatactgctc ccataacaag acaggggagc agacaatcat ggcaatttca
      121 tcgcgtaaca cacttcttgc cgcactggca ttcatcgctt ttcaggcaca ggcggtgaac
      181 gtcaccgtgg cgtatcaaac ctcagccgaa ccggcgaaag tggctcaggc cgacaacacc
      241 tttgctaaag aaagcggagc aaccgtggac tggcgtaagt ttgacagcgg agccagcatc
      301 gtgcgggcgc tggcttctgg cgatgtgcaa atcggcaacc tcggttccag cccgttagcg
      361 gttgcagcca gccaacaggt gccgattgaa gttttcttgc tggcgtcaaa actgggtaac
      421 tccgaagcgc tggtggtaaa gaaaactatc agcaaaccgg aagatctgat tggcaaacgc
      481 atagccgtac cgtttatctc taccacccac tacagcctgc tggcggcgct gaaacactgg
      541 ggcattaaac ccgggcaagt ggagattgtg aacctgcagc cgcccgcgat tatcgctgcc
      601 tggcagcggg gagatattga tggtgcttat gtctgggcac cggcggttaa cgccctggaa
      661 aaagacggca aggtgttgac cgattctgaa caggtcgggc agtggggtgc gccaacgctg
      721 gacgtctggg tggtgcgcaa agattttgcc gagaaacatc ctgaggtcgt gaaagcgttc
      781 gctaaaagcg ccatcgatgc tcagcaaccg tacattgcta acccagacgc gtggctgaaa
      841 cagccggaaa acatcagcaa actggcgcgt ttaagcggcg tgccggaagg tgacgttccg
      901 gggctggtga aggggaatac ctatctgacg ccgcagcaac aaacggcaga actgaccgga
      961 ccggtgaaca aagcaatcat cgacaccgcg cagtttttga aagagcaggg caaggtcccg
     1021 gctgtagcga atgattacag ccagtacgtt acctcgcgct tcgtgcaata aaaggaggcg
     1081 cagatgctgc aaatctctca tctctatgcc gattatggcg gcaaaccggc actggaagat
     1141 atcaacctga cgctggaaag cggcgagcta ctggtggtgc tggggccgtc cggctgcggt
     1201 aaaaccaccc tgctgaatct gattgccggt tttgtgcctt atcagcatgg cagcattcaa
     1261 ctggcgggta agcgtattga gggaccggga gcagagcgcg gggtggtttt tcagaatgaa
     1321 gggctactac cgtggcgcaa tgtacaggac aacgtggcgt tcggcctgca attggcaggt
     1381 atagagaaaa tgcagcgact ggaaatcgcg caccagatgc tgaaaaaagt ggggctggaa
     1441 ggcgcagaaa aacgctatat ctggcagctt tccggtggtc aacgtcagcg ggtggggatt
     1501 gcccgcgcgc tggcggcgaa tccccagctg ttattactcg acgaaccgtt tggtgcgctg
     1561 gacgccttca cccgcgacca gatgcaaact ctgctgctga aactctggca ggagacgggc
     1621 aagcaggtgc tgttgattac ccacgatata gaagaagcgg tgtttatggc gactgaactg
     1681 gttctgcttt catccggccc tggccgtgtg ctggagcggc tgccgctcaa ctttgctcgc
     1741 cgctttgttg cgggagagtc gagccgcagc atcaagtccg atccacaatt catcgccatg
     1801 cgcgaatatg ttttaagccg cgtatttgag caacgggagg cgttctcatg agtgtgctca
     1861 ttaatgaaaa actgcattcg cggcggctga aatggcgctg gccgctctcg cgtcaggtga
     1921 ccttaagcat tggcacgtta gcggttttac tcactgtatg gtggacggtg gcggcgctgc
     1981 aactgattag cccgctgttt ttgccgccgc cgcaacaagt actggcaaaa ctgctcacca
     2041 ttgccggacc gcagggattt atggacgcca cgctgtggca gcatctggca gccagcctga
     2101 cgcgcattgt gctggcgcta ctcgcggcgg tggtgatcgg tattccggtc gggatcgcca
     2161 tggggctaag cccgacggtg cgcggcattc tggatccggt aatcgaactt tatcgtccgg
     2221 tgccgccgct ggcttatttg ccgctgatgg tgatctggtt tggtattggt gaaacctcga
     2281 agatcttact gatctattta gcgatttttg caccggtggc gatgtcggcg ctggcggggg
     2341 tgaaaagcgc gcagcaggtt cgcattcgtg ccgcccagtc gctgggtgcc agccgtgcgc
     2401 aggtgctgtg gtttgtcatt ttgcccggcg cgctgccgga aatcctcacc ggattacgca
     2461 ttggtctggg tgtgggctgg tctacgctgg tagcggcgga gctgattgcc gcgacgcgcg
     2521 gtttaggatt tatggttcag tcagcgggtg aatttctcgc gactgacgtg gtgctggcgg
     2581 gaatcgcggt gattgcgatt atcgcctttc ttttagaact gggtttgcgc gcgttacagc
     2641 gccgtctgac gccctggcat ggagaagtac aatgagtgaa cgtctgagca ttaccccgct
     2701 ggggccgtat atcggcgcac aaatttcggg tgccgacctg acgcgcccgt taagcgataa
     2761 tcagtttgaa cagctttacc atgcggtgct gcgccatcag gtggtgtttc tgcgcgatca
     2821 agccattacg ccgcagcagc aacgcgcgct ggcccagcgt tttggcgaac tgcatattca
     2881 ccctgtttac cctcatgccg aaggggttga cgagatcatc gtgctggata cccataacga
     2941 taatccgcca gataacgaca actggcatac cgatgtgaca tttattgaaa cgccacccgc
     3001 aggggcgatt ctggcagcta aagaattacc ttcgaccggc ggtgatacgc tctggaccag
     3061 cggtattgcg gcctatgagg cgctctctgt tcccttccgc cagctgctga gcgggctgcg
     3121 tgcggagcat gatttccgta aatcgttccc ggaatacaaa taccgcaaaa ccgaggagga
     3181 acatcaacgc tggcgcgagg cggtcgcgaa aaatcctccg ttgctacatc cggtggtgcg
     3241 aacgcatccg gtgagcggta aacaggcgct gtttgtgaat gaaggcttta ctacgcgaat
     3301 tgtcgatgtc agcgagaaag agagcgaagc cttgttaggc tttttattcg cccatatcac
     3361 taaaccggag tttcaggtgc gttggcgctg gcaaccgaat gatattgcga tttgggataa
     3421 ccgcgtgact cagcactatg ccaatgccga ttacctgcca cagcggcgga ttatgcatcg
     3481 ggcgacgatt cttggggata aaccgtttta tcgggcgggg tgatacgagt gcggacggtt
     3541 ccctcgcccc cttggggaga gggttagggt gagggggcgt tcaccgtact ttcaacaggt
     3601 taactccccc tttctgagag gaaaaaaatt aacgcagaat cttcttctca gccaaatcca
     3661 gcgcaaagta gctgaaaatc agatccgcac ccgcacgctt aattgaacct aagctttcga
     3721 gcacgacttt ctcttcatct atagcacccg ccagcgcggc gaacttaatc atcgcgtact
     3781 caccgctcac ctgatacgcg ccaatcggca attcagtacg ttcacgcagc tcacgcacga
     3841 tgtcgaggta cgctccagca ggtttaacca tcaggcagtc tgcgccctgg gcttcatcca
     3901 gcagtgactc acgaatcgcc tcgcgacggt tcattgggtt catctgatag cttttgcggt
     3961 cgccttttaa tgcgcttccg gcagcttcac ggaacgggcc ataaaaggaa gaggcgaact
     4021 tggtcgaata cgacataatc gccgtatctt taaagcctgc agcgtccagc gcctgacgaa
     4081 tcgcctgtac ctggccgtcc atcgcggcgg aaggggcgat gaagtctgcg cctgcagcag
     4141 ctgcaaccac ggcttgcttg cctaaatttt ccagagtcgc gtcgttgtcg acgccatgct
     4201 cgcacagcac accgcagtga ccgtgagaag tgtattcaca gaaacaagtg tctgacatga
     4261 cgatcatttc tggcacagtc tgcttgcaga tgcgcgacat acgcgccacc agtccatctt
     4321 cccgccaggc gtcgctgccg gtttcatcgg tatggtgaga gatgccgaaa gtcatcacgg
     4381 aacgaatacc ggcgttggcg atgcgttcaa tttcgcgtgc cagatgtttc tctggaatgc
     4441 gcatcacgcc tggcatggct tcaacggctt tgtagtcgtc aatttcttct tcaacaaaga
     4501 tcggcaacac caggtcgtta aggctaagtg ttgtctcttc aaacatagcg cgcagcgcag
     4561 gagatttgcg caggcgacga gggcgttgga ttaagtctgt catggtctgc ctgatgtttg
     4621 tggaattaag gtgcacagta tacctgaagc ggggcaggag tgttttacga aagttgtcgt
     4681 gatgttgaca agaagagaat gaaaga
//
LOCUS       DAPHBJ010000262         4582 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.264, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000262 DAPHBJ010000000
VERSION     DAPHBJ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.264"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..600
                     /locus_tag="RHZ76_004248"
     CDS             <1..600
                     /locus_tag="RHZ76_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDV2814767.1"
                     /translation="GIGYAVIRRTIFIVYPPILSNGMFNFIVMQTLFYLPFFILGALA
                     FIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETESVITMVLGLWMVN
                     VVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHITSNWLGFLCGLI
                     FVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(608..2089)
                     /gene="clsC"
                     /locus_tag="RHZ76_004249"
     CDS             complement(608..2089)
                     /gene="clsC"
                     /locus_tag="RHZ76_004249"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HDV2814768.1"
                     /translation="MMKKTPTSTKDSLLNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2031..2564)
                     /gene="ymdB"
                     /locus_tag="RHZ76_004250"
     CDS             complement(2031..2564)
                     /gene="ymdB"
                     /locus_tag="RHZ76_004250"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HDV2814769.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSFTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(2659..2970)
                     /locus_tag="RHZ76_004251"
     CDS             complement(2659..2970)
                     /locus_tag="RHZ76_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV2814770.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTVQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(3091..3423)
                     /gene="csgC"
                     /locus_tag="RHZ76_004252"
     CDS             complement(3091..3423)
                     /gene="csgC"
                     /locus_tag="RHZ76_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HDV2814771.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTRLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(3482..3937)
                     /gene="csgA"
                     /locus_tag="RHZ76_004253"
     CDS             complement(3482..3937)
                     /gene="csgA"
                     /locus_tag="RHZ76_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HDV2814772.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGANVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(3978..4433)
                     /gene="csgB"
                     /locus_tag="RHZ76_004254"
     CDS             complement(3978..4433)
                     /gene="csgB"
                     /locus_tag="RHZ76_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HDV2814773.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQEGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         1165 a         1139 c         1016 g         1262 t
ORIGIN      
        1 ggcatcggtt atgcggtaat aagaagaacg atttttattg tgtatccgcc cattctgagt
       61 aatggcatgt tcaattttat tgtcatgcaa acgctatttt atttaccgtt ctttatcctc
      121 ggcgcactgg ctttcatttt ccctcatctt aaagccttgt ttaccacgcc gtctcgtggc
      181 tgtaccctcg ctgcagcatt ggcgtttgtc gcttatttac tcaaccagcg ctatggcagt
      241 ggcgatgcct ggatgtacga aaccgagtca gtgatcacca tggtcctcgg tctgtggatg
      301 gtgaatgtgg tcttctcctt tggccaccgt ttgcttaact tccagtcagc gcgggtgact
      361 tactttgtta acgcatcgct atttatctat ctggttcacc acccgttaac gctgtttttc
      421 ggcgcataca ttacaccgca catcacctcc aactggcttg gttttctctg tggcctgata
      481 ttcgtagtag ggattgcgat aattctgtat gaaattcatt tacgcatccc gttactgaag
      541 tttttgtttt ctggtaaacc ggttgttaag cgtgagaacg ataaagcacc agcccgttaa
      601 gccacattta caataaccat tccacgggca atatcgacgc cagtctgacc ataacccgct
      661 tccagaaact ggtggcgggt tcttttttga gaataatctc tttctttgca tgacgatcaa
      721 cccagttgat ccgtccccac ctgtccagac ggagctgcca ggccgcatca tactggctct
      781 gaataaagcg tttatcaatt aactgtgcca gcgtttcgct ctctatcacg aagcccattt
      841 cagtattgag caatgttgaa cgcggatcga aattgaaaga gccgataaac accgttttac
      901 catcgatgct aaaagtttta gcatgcaggc tagctccgga attaccggtt atgccgcgat
      961 cgtgtaacgt actactttgt tcacgcgtcg gcttgagttc atataattcc acgccatagc
     1021 ggagcaattt tttgcgccag cgcgcgtatc cggcatggac gacagcaaca tcgttagcgg
     1081 caagagaatt ggttaggatc gcaatcttta ccccttttct caccatccgt aagagttgcg
     1141 ccacacctgc gcgtgtcggt acaaaatagg aagagataat atcgatgcgt tcactgggtg
     1201 agcccatgat atcgaacagg cgctgcggta acagtgaatg ccgttttgcc ttgccctccc
     1261 ctttcgccgg atcatcactt aataaacgtg ttttcgccca gataagcggc aacgttccat
     1321 caaccagatg atttataaac ggactggatt ccatttggcg taagtaacga tgcgtcatgg
     1381 catcgttatg ccaggaggcg ggtaactcga tgcgatccgc catttcacct tccgggacat
     1441 ccagcacctg ctgtaagggt gaaaccgatt tgcaatacca gtagcgggcg aaatcatcgg
     1501 caacgtcctc taccacgggt cctattgcca taacatctaa atccgaaaaa agtggctctt
     1561 cccctgcccc aaaataggca tcaccaatat ttcgtcctcc caccagggta accacgccat
     1621 cgacagtgaa acttttattg tgcatacggc gattgagacg ggaaaagtcg gtgatataac
     1681 caagcggacg cagcaagcga aacgagaaag gattaaaaag ccggacttca atgcggggat
     1741 ggctgtcaag caagcgtaaa atatcgtcaa gtccgggcgt attgttgtcg tccagcaaca
     1801 aacggacgcg aacgccacgc tttgctgcgg ctaacagggc ggaaaacagt aaccgacccg
     1861 acatatcgtc ctgccagatg taatactgaa catccagcgt atgctctgcc atttcggcca
     1921 gacgataccg ggctgcaaac gcatccagac ttttctccag aggaaaaagg ccacactgac
     1981 cgggatgttg cgaacacagt ggcagcaccg cgctcgccag ccggggcaaa tcattcatct
     2041 ccttgttgag taaggagtct ttcgtagagg tgggcgtttt cttcatcata acagacaaag
     2101 tatacctgtt cgggtaaagc gtgacgggta ataaattctg aaacggtttt tactgcgatt
     2161 tcagccgctg ccgcacgagg gtaaccataa accccagtac tgattgcagg aaaagccact
     2221 gacgtaaagc tgtttgccgc caccagtcgt aggctattga gataggcatc ctgcaaaagc
     2281 tggtcttcat tttgttcacc accacgccag actggcccga cggtgtgcac tacggcttta
     2341 gcaggaagat cgcctgcaag cgtaataacg gcatgccccg tagggcaatc gccctgctgt
     2401 tgcctgactt ttaaacaagc atccagcagg gccggacccg ctgcgcgatg aatggcccca
     2461 tcgacgccgc cgcctcccat taatgacgga ttagccgcat tcacaatcac atcaacggcc
     2521 agtttggtaa tatcaccctg cacaacatga atacgcgttt tcataacgcc tccttacacc
     2581 ttcttgtttc ttaagtgtac agcaggcttt ttggttaaat cgcatttttc tgctcagcaa
     2641 cgtgagttgt tcagaaactt agttatattc gatgttcatt acagccagct ttttctgttt
     2701 atcaagatac tgcactttta ctgaggctat ttgcgcgttt ttgacattgc cgctcattaa
     2761 cgctttgctg gagtaggttt tgcccggaat agaaccattc tgtgggcagt tcatttctac
     2821 gggtgatgac tgggtgctga cgtcacatgc cggttccaca atttggccat aaaaatggat
     2881 cacgccccct tgcacggtgc ttccagcaat ggcaataaaa ggaaaaaaca aactgccgag
     2941 cataagagag tttaaaaaag gtctgaacat ttattctatt tcctcaatga ttagtcatcc
     3001 ttgagggttg tgttatccat actttcaggt tggctgattc gcagcagacc attctctcca
     3061 gattcatctt atgctcgata tttcaacaaa ttaagacttt tctgaagagg gcggccattg
     3121 ttgtgataaa tgaagtgact gtccatcaga aacagtaaca actattttca cccgatcgtc
     3181 cggggaaata tttaaactca acctcgtcaa agcaatgggt tgattagcag gcaatgaaag
     3241 ggtcttttct tgcttcgtct gactttgccc tgaactgcct tcgcgcaggg acaatatttg
     3301 tactctgcac agacaagatt gagtaagagt gacttcagga ataatggtat acacatcccc
     3361 ttgctgggtc gtattaaagg ttatctgact ggaaagtgcc gcaaggagta ataacgcatt
     3421 catattcttc tcccgaaaaa aaacagggct tgcgccctgt ttctgtaata caaatgatgt
     3481 attagtactg atgagcggtc gcgttgttac caaagccaac ctgagtcacg ttgacggagg
     3541 agttagatgc agtctggtca actgcagcac cgttgccacc accgaactgt ttaaccgtca
     3601 tttcagaatt tttgccgttc cactgatcaa gagtagcgct gttaccgaag ccacgttggg
     3661 tcagatcgat tgagctgtca tctgagccct gaccaacatt tgcaccatta ccgccgccat
     3721 gctgggtaat agtcaagtca gagttacggg catcagtttg cagagcaagt gcagagttac
     3781 cgccaccgta ctggtaaatg ttcagctcag aatttgggcc gctattatta ccgccaccac
     3841 cgtggttacc gccgccgccg tactgaggaa caacacctgc cagagcgcta ccggagaata
     3901 cgattgctgc aattgctgct acttttaaaa gtttcatgta aaacccccat cggattgatt
     3961 taaaagtcga atggaaatta acgttgtgtc acgcgaatag ccatttgcga ctgtctctgc
     4021 actacaattg ccgttttttg agtaccatac tgtgtaatat ttgctttatt accagaacct
     4081 ttctggataa tcatcgcagt attaccataa gcaccttgcg aaatactggc atcgttggca
     4141 ctgcccgcct gatcaatata tgcaaggtta taatctcctg tctggtcaat ctttgcccgg
     4201 ttgctactac cttcttgcgc aacaaccgcc aaaagttttg agccttcctg ccgtaactga
     4261 gcactattat tagtcccagc ttgaccaatt atggctgcct gattaaatga agacttactc
     4321 aattcattta ccgcgaagtt atattctgaa ttagctaaat cataacctgc tgcggctgca
     4381 atcccaggcg cacccagtat tgttaacatc ataaataaca atttgttttt catgttgtca
     4441 ccctggacct ggtcgtacat ttaagaaatt aaatcatttc aacttggttg ttaacgcaac
     4501 ctgtattttg ttaacgctgc gttacgatgg aaagtatgtc tgcggaaata tttttaataa
     4561 ctcacccgcg cgttgtattt tt
//
LOCUS       DAPHBJ010000263         4509 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.265, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000263 DAPHBJ010000000
VERSION     DAPHBJ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.265"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..234)
                     /locus_tag="RHZ76_004255"
     CDS             complement(<1..234)
                     /locus_tag="RHZ76_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HDV2814774.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFY"
     gene            complement(237..956)
                     /gene="sanA"
                     /locus_tag="RHZ76_004256"
     CDS             complement(237..956)
                     /gene="sanA"
                     /locus_tag="RHZ76_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HDV2814775.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(1106..1990)
                     /gene="cdd"
                     /locus_tag="RHZ76_004257"
     CDS             complement(1106..1990)
                     /gene="cdd"
                     /locus_tag="RHZ76_004257"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDV2814776.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(2120..2815)
                     /locus_tag="RHZ76_004258"
     CDS             complement(2120..2815)
                     /locus_tag="RHZ76_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HDV2814777.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(2812..3210)
                     /locus_tag="RHZ76_004259"
     CDS             complement(2812..3210)
                     /locus_tag="RHZ76_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDV2814778.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            3449..4396
                     /gene="dusC"
                     /locus_tag="RHZ76_004260"
     CDS             3449..4396
                     /gene="dusC"
                     /locus_tag="RHZ76_004260"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HDV2814779.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGDIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
BASE COUNT         1199 a         1145 c         1163 g         1002 t
ORIGIN      
        1 atagaaaata agataaacaa tcatgccgat agataaaatt ccgaagccga tatatgccac
       61 catataagcg aaaaagagtc cgggattttt tgctcgccag acatacaccg gttccggtgc
      121 atttttaacc cgtttatcaa tcgcgcctgc gcgccagcct ttccatgctt cgcggaaaca
      181 aaatatcggg ataaggaaaa aaacggcaac gacaaaaaac tgctgtacat ccataactac
      241 tttccttgtt tcttttgtaa ttcaagtaac tgttcgggtg tgacggcggg atacccctgc
      301 gcatcttccg gtacctggtg catagccgga atagggacca gcggccctaa aaaacgcggt
      361 tcacgtttaa aaatataaag gtcagccagc gcaccgaaac gggcggcaaa ttcacgaata
      421 cgtactgaca gcatatcttt cggtgacggt acggcataac actgagcctg aatccccata
      481 tgcagcgcaa taaataatgc tcgctcacag tggaaacgtt gggtgataat aatgaaatca
      541 ttagtatcga aaactttgcg tgtacgcacg atggaatcca gcgtacgaaa gcctgcgtaa
      601 tcgagaacaa tatctgatgg gtcgacacca gcagcgatta aatctttgcg catggtcatc
      661 ggctcattat aactttgcaa tgcgttatcg ccgctcagta atagataatt taccttaccg
      721 ctgttatagg cattaatcgc tccttgaatg cggtagcgat aatactgatt aattacgcca
      781 gtacgataat attttgctgt tccgagcacc acaccgacct ggcggtaggg gagatcctgc
      841 aattcgtcgt agatataagg cgcggttttc cagctcatcc agcgatcgag gccgagcaca
      901 gtcaacagca gcaagccgat caggactaac aggctgagga acacgcgctt taacatgcag
      961 gagactcgat ctaaggaaaa tttccatcag gctactttac ccgctggttt tgcgcaagaa
     1021 acggcaaact gtttgccggg attttcgaca ccaggagagg gctggcaagc cggacaaggc
     1081 gttcacgccg catccggcac caggcttaag cgagaagtac gcggtcgata ctgtgacagc
     1141 cgagagcttt cagcgttgcg gaggtggcat cccactgaat caacggcgca tcggcttttt
     1201 ctgccagaac cgcgcgctgg atatccgggt aatcataacc cttgagattc aatagaatta
     1261 acgctccctg caacggtggc agagtcgggt tgaatgcggc gttttcagcg tagctgccgc
     1321 tgaaaattcg accgtcttta cattccagcg caacaccgct tggcgactta ctgtaaggca
     1381 tgtgcgaacg gtttgccgcc gcaatcgccg cctgagaaag cgcatcacca gtcagcgcat
     1441 agccgtgatc ctgctcgtcc atcagcagcg ttttaatctc cagatctttc ggcccaaagg
     1501 catccggcag atagtcacgc agcgcgtgtg cctcgcggcc cggcagatga atacgcagat
     1561 ccagaccgct gttcagctca ttcataaact gacggcagtg accacaaggc gtgtagttaa
     1621 cggtgatggc tgcaagcgct ttttcaccac tcaaccaggc gtggctgatc gcgctttgtt
     1681 cggcatgaac ggtttgctgc attgtcgcac caataaattc catattggca ccaaaatacc
     1741 aggttccgct cacaccgcgc gcaattgcgc caacattaaa attcgacaat ggcgtacgcg
     1801 cacaggccgc cgccagcgga agtagtgcga atgccagcgc gtcttcgtcc agccccgttg
     1861 cgctcttcag cgatgagact tgctccccgg tcaacaaagc ggggaagtac ttgtctgcca
     1921 gaataggttc cagtgcagat tgcaagttat ccgcaagttg ggcaaaagcg gtttgaaaac
     1981 gtggatgcat ggcgttgcct cataatcatg taatgggata acagtttacg aacccgttat
     2041 ggctttatat gtgatctgaa tctcattaat tatgcaacgc gtgaaactta catcaaaaat
     2101 gtgcgacgca tcgcaaattt tagcccatta ctgccagaat aatcgggaaa aggaacggtg
     2161 cgatcagcga agtaattatc ccgcataaca ccagcgctag cgaactaaat gcaccttcct
     2221 gataatccag ctcggcgcag cgcgccgtac cgagggcgtg cgaggcagtc cccattgcca
     2281 gaccgcgcgc agctttggta cgaatacgca tcgcattaag caatgtatgg ccaaataccg
     2341 cgccgaggat gccgacgaaa atcacgcaaa ctgcgctgat tgccggaata ccgccaatac
     2401 tgccgccaac cgccattgca ataggcgtgg tgactgattt cggcaggatt gacgcggcga
     2461 tttccggtga agcgcccatc aacaatgcca cggaagtccc cgtcaccatc gcaaccacgc
     2521 tgccgataaa acagatggtg atgatcgatt tccagcgcgc gcggatctgg tgtagctgct
     2581 catataaagg ataggccagc gcgacaaccg ccggttgcag cagatcgttt agcacttcgc
     2641 tacctttaaa gtagctgtcg taagagatgc cggtcagcat taaaaaaggg atgatcacca
     2701 ccattgctac cagcaacggg ttaagcaagg gaaacttata ccgtgccgcc agtttgcggg
     2761 cggcaaaaaa gacaatcaat gtcagtggta atgaccacca gatattcgcc atcattcttc
     2821 tgatcctttc tgacctacga ctttacgttc accgtgcacc agttgcgaac tccagctcac
     2881 caccagaaaa accaccagcg tactgacagc acaggaaacc actaccgggc caaactgtgc
     2941 gcggagcaaa tcaaaatatt gcatgacgcc tacgccaatc ggcacaaaca atagcgccat
     3001 atagcgaatc agtacgtagc accccggatt gacccatttt gccggaagaa tttgcaaggc
     3061 cagcaggaca aacaggatca gcatcccgat gatgctgccc ggaatggtta ccggtagcag
     3121 ggaagcaatg aaaatgcctg catacaggca ggcataaatc aggacgaaag cgcgtaaata
     3181 ttgccagata atgttcagtg tcttactcat aatcaacacc ccagatgaga tggattcatc
     3241 atacaattaa ttcgtaaaac gtgctaccga tcacattaag aaatcagcgc ttattttttc
     3301 gctccccggt attacgcaat gagcagcgaa ctcaggcata catcaagcat agagagggat
     3361 tgcagtttgc agcaatccct ctttgcagac cttcaatccg ctgaccatag gcgctaacat
     3421 agcgcctcat ttttttgcgg gtgatgatat gcgtgtgtta ctggcaccga tggagggagt
     3481 gcttgactct ctggtgcgtg aattgctgac cgaagttaac gactacgatc tgtgcatcac
     3541 cgagtttgtc cgcgtggtgg atcaactgct gccggtaaaa gtctttcatc gcatttgccc
     3601 tgagctacaa aacgccagcc ggacaccatc tggtacgctg gtgcgcgtgc agttgttagg
     3661 tcagttccca caatggctgg cagagaacgc cgcccgtgcg gtggagttag gttcctgggg
     3721 cgtggatctc aattgcggct gcccgtcgaa aacggttaac ggtagcggcg gcggcgcgac
     3781 gttactcaaa gatcctgaac tcatctacca gggtgcaaaa gcgatgcgtg aagctgtacc
     3841 agcgcatttg cccgtcagcg tgaaagtgcg tctgggctgg gacagcggtg agaagaaatt
     3901 tgaaatcgcc gatgcggttc aacaggctgg cgctacggag ctggtggtgc atgggcggac
     3961 gaaagagcag ggttaccgcg cggagcatat tgactggcag gcgattggcg atattcgcca
     4021 gcggctgaat attccggtga ttgccaacgg tgaaatctgg gactggcaga gcgcgcaaca
     4081 atgcatggcg atcagcggct gcgacgcagt gatgattggt cgcggggcgc tcaatattcc
     4141 caacctgagc cgggtggtaa aatataacga accgcgaatg ccgtggccgg aggtggttgc
     4201 tttgctgcaa aaatataccc gtctggaaaa gcagggcgat accgggttat atcacgttgc
     4261 gcggattaaa cagtggttga gttatttgcg taaagaatac gatgaagcaa cggaattatt
     4321 tcagcatgtt cgggtgttga ataattcccc tgatattgca agggctattc aggcaattga
     4381 tatcgagaaa ctctaaaaaa ccatagtgat gttttttgac aatgtgttag ctctttatca
     4441 ataggccgca cggttgtttg gtggcgtcaa aattgccgag gcgttccctg aaggccgggg
     4501 cagcccaca
//
LOCUS       DAPHBJ010000264         4495 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.266, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000264 DAPHBJ010000000
VERSION     DAPHBJ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.266"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..482)
                     /locus_tag="RHZ76_004261"
     CDS             complement(<1..482)
                     /locus_tag="RHZ76_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDV2814780.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     "
     gene            complement(597..672)
                     /locus_tag="RHZ76_004262"
     tRNA            complement(597..672)
                     /locus_tag="RHZ76_004262"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(637..639),aa:Thr,seq:ggt)
     gene            complement(679..753)
                     /locus_tag="RHZ76_004263"
     tRNA            complement(679..753)
                     /locus_tag="RHZ76_004263"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(718..720),aa:Gly,seq:tcc)
     gene            complement(870..954)
                     /locus_tag="RHZ76_004264"
     tRNA            complement(870..954)
                     /locus_tag="RHZ76_004264"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(918..920),aa:Tyr,seq:gta)
     gene            complement(963..1038)
                     /locus_tag="RHZ76_004265"
     tRNA            complement(963..1038)
                     /locus_tag="RHZ76_004265"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1003..1005),aa:Thr,seq:tgt)
     gene            1400..2350
                     /gene="coaA"
                     /locus_tag="RHZ76_004266"
                     /gene_synonym="panK"
     CDS             1400..2350
                     /gene="coaA"
                     /locus_tag="RHZ76_004266"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDV2814781.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2379..3344)
                     /gene="birA"
                     /locus_tag="RHZ76_004267"
     CDS             complement(2379..3344)
                     /gene="birA"
                     /locus_tag="RHZ76_004267"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HDV2814782.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3341..4369)
                     /gene="murB"
                     /locus_tag="RHZ76_004268"
     CDS             complement(3341..4369)
                     /gene="murB"
                     /locus_tag="RHZ76_004268"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDV2814783.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAEALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1147 a         1128 c         1019 g         1201 t
ORIGIN      
        1 tactgagaca gaagttcacg aacttccatt tcaaccagtt ccagcagctc ttcgtcatca
       61 accatgtcgc atttgttcag gaacacgatg atgtacggaa cgcctacctg acgacccagc
      121 aggatgtgct cacgagtctg cggcatcggg ccgtcagtcg cagcaactac caggatcgcg
      181 ccgtccatct gagcagcacc ggtgatcatg tttttaacat agtcggcgtg ccccgggcag
      241 tctacgtgtg cgtagtgacg ggtcggggtg tcgtattcaa cgtgagaagt gttgatggtg
      301 ataccacgag ctttttcttc cggcgcgtta tcgatctggt cgaatgcgcg agcagcaccg
      361 ccgtaggttt tagccagtac ggtagtgatt gcagcggtca gcgttgtttt accatggtca
      421 acgtggccga tagtaccgac gttaacgtgc ggttttgtac gttcaaactt ttctttagac
      481 atcgattgtc cctctaagac acggataaat cggtgatatc accacatcaa ccaggcaaca
      541 tgcccgactt gttgaatgca ataaacagaa ggaaaaaaca gggaggagaa aagaagtggt
      601 gctgataggc agattcgaac tgccgacctc acccttacca agggtgcgct ctaccaactg
      661 agctatatca gcacatcttg gagcgggcag cgggaatcga acccgcatca tcagcttgga
      721 aggctgaggt aatagccatt atacgatgcc cgcatcctgg aactcggcta cctgattttc
      781 attctgcact gaatatcgag agaagctctc ttcattcgag ccggtaagcg aacttatcgt
      841 ctcgggctac gccatcgcgt ggccgaaatt ggtggtgggg gaaggattcg aaccttcgaa
      901 gtctgtgacg gcagatttac agtctgctcc ctttggccgc tcgggaaccc caccggactt
      961 gatggtgccg actaccggaa tcgaactggt gacctactga ttacaagtca gttgctctac
     1021 ctactgagct aagtcggcat caagtagcgc gcattctatg gagacatgcg agttcatgca
     1081 actaaaaaat tgcataattt gttttattgg tcacatttta tgcgacacga tgaagaaaca
     1141 gccgaacgga gggcagatta gcacactttt tcaacatcat tgtgctcaac aatgcgctcc
     1201 tgctaaacca taattctttt tatcagatgg aatatctgtc acattgcttt tcaacgatag
     1261 cttcctggca gagatttttt cttattattc ctccccatct ggtgttaccc tcctgcccat
     1321 taacccattc aacagaactg tgacgcgcca tggcaaatat cgctttgccg atagagctat
     1381 gaccgccaga aacatgctta tgagtataaa agagcaaacg ttaatgacgc cttacctaca
     1441 gtttgaccgc aaccagtggg cagctctgcg tgattccgta cctatgacgt tatcggaaga
     1501 tgagatcgcc cgtctcaaag gtattaatga agatctctcg ttagaagaag ttgccgagat
     1561 ctatttacct ttgtcacgtt tgctgaactt ctatataagc tcgaatctgc gccgtcaggc
     1621 agttctggaa cagtttcttg gtactaacgg gcaacgcatt ccttacatta tcagtattgc
     1681 tggcagtgtc gcggtgggga aaagtacaac cgcccgtgta ttgcaggcgc tattaagccg
     1741 ttggccggaa catcgtcgtg ttgaactgat cactacagat ggcttccttc accctaatca
     1801 ggttctgaaa gaacgtggtc tgatgaagaa gaaaggcttc ccggaatcgt atgatatgca
     1861 tcgcctggtg aagtttgttt ccgatctcaa atccggcgtg ccaaacgtta cagcccctgt
     1921 ttactcgcat cttatttatg atgtgatccc ggatggagat aaaacggttg ttcagcctga
     1981 tattttaatt cttgaagggt taaatgtctt acagagcggg atggattatc cacacgatcc
     2041 acatcatgta tttgtttctg attttgtcga tttttcgata tatgttgatg caccggaaga
     2101 cttacttcag acgtggtata tcaaccgttt tctgaaattc cgcgaagggg cttttaccga
     2161 cccggattcc tattttcata actacgcgaa attaactaaa gaagaagcga ttaagactgc
     2221 catgacattg tggaaagaga tcaactggct gaacttaaag caaaatattc tacctactcg
     2281 tgagcgcgcc agtttaatcc tgacgaaaag tgctaatcat gcggtagaag aggtcagact
     2341 acgcaaataa tttgcagggg agcgaatact cccctttctt atttttctgc actacgcagg
     2401 gatatttcac cgcccatcca gggttttatt attccatcct gctcaagtaa taaagccccc
     2461 tgtttgtcta ttccgcgtga aatgccaaat atttctttat caccaatgat aagtttcact
     2521 gggcgattaa taaaattatc cagcttttcc cagcgcgaca gataaggtgc caatccttct
     2581 tgttcgaaga gttccaacgc agcacgtaat tcacgtatta gcatggccgc caacgtatta
     2641 cgatcgagat tgatccccgc ttcctgcagc gtgatccacc cctgattaac gacactctct
     2701 tcaacacggc gcattgccat gttgatcccg gctccaatga ctatttgcgc cgcatcgcca
     2761 gttttgccag tcagctccac aagaatgcct gccagcttgc gatcctgcag atagaggtca
     2821 ttaggccatt tgacacgaac tttatctgct cccagcttgc gtaatacttc cgccatcacg
     2881 ataccgataa ccagacttaa accaatcgcc gccgccgggc cttgttccag acgccagaac
     2941 atcgacaaat ataagtttgc gccaaaaggc gaaaaccatt tccgcccccg gcgaccacgg
     3001 ccagcctgct ggtattctgc aacacaggca tcgcccgatt taagctctcc gatacgatca
     3061 agaaggtact gattcgtgga gtcaataact ggcagcacgg ctacactacc gccatccagc
     3121 tgacccaata tctgttcagc attaagtaac tggatgggct caggcaggct gtatccttta
     3181 cccggaacgg taaagacatc aacgccccag tcacgcagtg tctgaatgtg tttattaata
     3241 gccgcccggc tcattcccag cgtttcaccc aactgctcgc cagagtgaaa ttcaccgttc
     3301 gctaacaggg caatcaattt cagtggcacg gtgttatcct tcatgaaatt gtctccactg
     3361 cgctcacttc acctgatgca ccaataaagc ggacttcagg ctcaagccag acattaaatt
     3421 tttcacccac tttttgtctt acatggtgtg ccagttgcac cacatcttcg ctttttgcat
     3481 tgtcttcatt aatgagaact aacgcctgtt gacggtgcac cgcagcccca cccatttgca
     3541 tcccttttag ctggcactga tcaataagcc aacctgctgc cagttttact gaaccatccg
     3601 cctgggggta atttggtgct gttggaaatt gtgacagtaa tgcttcagcc gtttcggcag
     3661 atacaacagg gtttttgaag aaactaccgg cattgccatt cacttttgga tcagggagtt
     3721 tggtggtgcg catatgacac accgcattaa atacttgctg tggcgttact gttgtaggat
     3781 ccagacgagt taagtcacca tacgttagta caggttgcca ctcttttggc agacgcagac
     3841 ctacggctac aatggcgaag cgatcctggt attcatgttt aaaaatactg tcgcgatagc
     3901 caaaacggca ctctttggca gttaagcgca cttgcttgcc tgtcgccagt tcaacacaat
     3961 caacataagc gcaaactcgc tgtaattcta cgccataagc accaatattc tggataggtg
     4021 atgagccgac acaaccagga attaatgcca gattttccag accaggcata ccttcctgca
     4081 aagtgtattt taccaggcga tgccagtttt ctccggctcc tacatgtaaa taccacgcat
     4141 caggttcatc atgaatttcg atacctttga tccggttgat gatcaccgtg ccgcgatagt
     4201 cttccagaaa aagtacatta cttccttcac ccagaataag aacgggttgt ccttctgcgg
     4261 ttgcatgctg ccaggcattg agtaattgtt gttcgtcttc ggcacataca atgtgctgag
     4321 cattatgatc aatgccaaat gtgttccagg gttttaagga gtggttcata gctgctttcc
     4381 tgatgcaaaa acgaggctag tttaccgtat ctgtgggggg atggcttgta gatatgacga
     4441 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgctt
//
LOCUS       DAPHBJ010000265         4436 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.267, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000265 DAPHBJ010000000
VERSION     DAPHBJ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.267"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            191..805
                     /gene="evgA"
                     /locus_tag="RHZ76_004269"
     CDS             191..805
                     /gene="evgA"
                     /locus_tag="RHZ76_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HDV2814784.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            810..4403
                     /gene="evgS"
                     /locus_tag="RHZ76_004270"
     CDS             810..4403
                     /gene="evgS"
                     /locus_tag="RHZ76_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HDV2814785.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTSTLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPEHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLKLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
BASE COUNT         1399 a          914 c          880 g         1243 t
ORIGIN      
        1 taaatcatcg gtacaatccc tgattttatt gttgacattt catttatgcc gactatttat
       61 atggtatact tgtcgaatta tcttaaagga agctcagatt ttcttatttt tattgagaaa
      121 atgagatgac gccttatgtc tgtattacta cagggagaag ggagatgctt cattgcaaag
      181 ggaataatct atgaacgcaa taattattga tgaccatcct cttgctatcg cagcaattcg
      241 taatttattg atcaaaaacg atattgaaat cttagcagag ttgactgaag gcggaagtgc
      301 cgttcagcgg gtggaaacac ttaagcctga tatcgtcatc attgatgtcg atatccccgg
      361 agttaacggt atccaggtgt tagaaacgct gaggaagcgc caatatagcg gaattattat
      421 tatcgtctcc gctaaaaatg accattttta cgggaaacat tgtgctgatg ctggcgctaa
      481 tggtttcgtg agtaaaaaag aaggcatgaa caatatcatt gcggctattg aagctgcaaa
      541 aaatggctac tgctatttcc ccttctctct caaccggttt gttggaagtt taacgtccga
      601 ccagcaaaaa ctcgactcct tatcgaaaca agaaattagt gtcatgcggt atattcttga
      661 tggcaaggat aataatgaca ttgctgaaaa aatgttcatc agcaacaaaa ctgtcagcac
      721 ttataaaagt cgcctgatgg aaaaattaga atgtaaatca ctgatggatc tttacacatt
      781 cgcacaacgt aacaaaatcg gctaaccaca tgaagttttt accctatatt tttcttctct
      841 gttgtggtct ttggtcgacc ataagtttcg cagacgaaga ttacatcgaa tatcgtggca
      901 tcagtagtaa caaccgtgtc acacttgatc cactacgtct gagcaacaag gaattacgtt
      961 ggttagcgag caaaaaaaat cttgtgattg cagtacataa gtcccaaacg tctacgttgt
     1021 tgcataccga ttcgcagcaa cgggttcgtg gtattaatgc tgattattta aatcttttaa
     1081 aaagagcgtt aaatatcaaa ttaacactcc gggaatacgc agatcatcaa aaagcaatgg
     1141 acgcgcttgc agaaggtgaa gtcgatatag tgttatcaca tttagttact tcgccgcctc
     1201 ttaataatga cattgctgca accaaaccat tgataattac ctttccggcg ctggtaacca
     1261 cccttcacga ctcaatgcga ccgcttacct caccaaaacc agtaaatatt gctcgggtag
     1321 caaattaccc cccagacgag gtaattcatc aatcatttcc aaaagcaaca attatctctt
     1381 ttacaaattt atatcaggca ttagcatccg tctcagctgg gcacaatgat tactttattg
     1441 gtagtaacat cattaccagc agtatgattt cccgctattt cactcactcc ttaaatgtag
     1501 tgaaatatta taactcgccg cgtcaatata attttttctt gaccagaaaa gaatctgtca
     1561 ttcttaatga agtactcaat agatttgttg atgctttaac aaatgaagtt cgctatgaag
     1621 tatcacaaaa ttggcttgat acaggaaacc tggcctttct gaacaaacca ttagaactca
     1681 ctgaacatga aaaacagtgg attaagcagc atcccaattt aaaggtgctg gaaaatcctt
     1741 actcgccccc ctattctatg acggatgaaa atggctcggt tcggggcgtt atgggggaca
     1801 ttcttaatat tattaccttg caaacaggtt taaatttttc tccgatcacc gtttcacaca
     1861 atatccatgc tggaacacag cttagccccg gaggatggga tataatacct ggcgctattt
     1921 atagtgaaga tcgagaaaat aatgttttat ttgctgaagc cttcataaca acgccttacg
     1981 tttttgtcat gcaaaaagcg cctgacagtg aacaaacatt aaaaaaagga atgaaagttg
     2041 ccattccata ttattatgag ctgcattcgc aattaaaaga gatgtatccg gaggttgaat
     2101 ggatacaggt cgataatgcc agcgctgcat ttcacaaggt taaggaaggt gaacttgatg
     2161 ctctggtcgc gacacagcta aattcgcgtt acatgatcga tcattactat cctaatgaac
     2221 tttatcattt tcttattcct ggcgttccga atgcatcgct ttcgttcgct tttcctcgcg
     2281 gagaaccgga acttaaggat attattaata aagcactgaa tgcaattccc ccaagcgaag
     2341 ttctgcgcct gacggaaaaa tggattaaaa tgcccaatgt gaccattgac acatgggacc
     2401 tatatagcga gcaattttat attgttacga cattatccgt tttattagtt ggcagtagcc
     2461 ttttatgggg attctacctg ttacgctcag ttcgtcgtcg taaagtcatt cagggtgatt
     2521 tagaaaacca aatatcattc cgaaaagcac tctcggattc cttaccgaat ccaacttatg
     2581 ttgtaaactg gcaaggtaat gtcattagtc ataatagtgc ttttgaacat tatttcactg
     2641 cggattacta caaaaatgca atgttaccat tagaaaacag tgactcaccc tttaaagatg
     2701 ttttttctaa tgcgcatgaa gtcacagcag aaacgaaaga aaatcgaaca atatacacac
     2761 aggtatttga aattgataat ggcatcgaga aaagatgcat taatcactgg catacattat
     2821 gcaatcttcc tgcaagtgac aatgcagtat atatttgtgg ttggcaagat attactgaaa
     2881 cgcgtgatct aattaatgca ctcgaggtag aaaaaaataa agcgataaag gctaccgtag
     2941 caaaaagtca gtttctggca acgatgagtc acgaaataag aacaccaata agctctatta
     3001 tgggcttcct ggaacttctg tcgggttctg gtcttagcaa ggagcaacgg gtggaggcga
     3061 tttcacttgc ctacgccacc ggacaatcac tcctcggctt aattggtgaa atccttgatg
     3121 tcgacaaaat tgaatcgggt aactatcaac ttcaaccaca atgggtcgat atccctactt
     3181 tagtccagaa cacttgtcac tctttcggtg cgattgctgc aagcaaatcg atcgcattaa
     3241 gttgcagcag tacgtttcct gaacattacc tggttaagat cgaccctcag gcgtttaagc
     3301 aggtcttatc aaatttactg agtaatgctc tcaaatttac caccgagggg gcagtaaaaa
     3361 ttacgacctc cctgggtcac attgatgaca accacgctgt tatcaaaatg acgattatgg
     3421 attctggaag tggattatcg caggaagaac aacaacaact gtttaaacgc tacagccaaa
     3481 caagtgcagg tcgtcagcaa acaggttctg gtttaggctt aatgatctgc aaagaattaa
     3541 ttaaaaatat gcagggcgat ttgtcattag aaagtcatcc aggcatagga acaacattta
     3601 cgatcacaat cccggtagaa attagccagc aagtggcgac tgtcgaggca aaagcagaac
     3661 aacccatcac actacctgaa aagttgagca tattaatcgc ggatgatcat ccgaccaaca
     3721 ggctattact caaacgccag ctaaatctat taggatatga tgttgatgaa gccactgatg
     3781 gtgtgcaagc gctacacaaa gtcagtatgc aacattatga tctgcttatt actgacgtta
     3841 atatgccgaa tatggatggt tttgagttga ctcgcaaact ccgtgagcaa aattcttcct
     3901 tacccatctg ggggcttaca gccaacgcac aggctaacga acgtgaaaaa gggttaagtt
     3961 gcggcatgaa cttatgtttg ttcaaaccgt tgaccctgga tgtactgaaa acacatttaa
     4021 gtcagttaca ccaagttgcg catattgcac ctcagtatcg ccaccttgat atcgaagccc
     4081 tgaaaaataa tacggcgaac gatctaaaac tgatgcagga gattctcatg actttccagc
     4141 atgaaacgca taaagatcta cccgctgcgt ttcaagcact agaagctggc gataacagaa
     4201 ctttccatca gtgtattcat cgcatccacg gtgcggctaa catcctgaat ttgcaaaagt
     4261 tgattaatat tagccatcag ttagaaataa cacctgtttc agatgacagt aagcctgaaa
     4321 ttcttcagtt gctgaactct gtaaaagaac acattgcaga gctggaccag gagattgctg
     4381 ttttctgtca gaaaaatgac taaatagcgg ctcccacaat gttcaaatgt gggagc
//
LOCUS       DAPHBJ010000266         4230 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.268, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000266 DAPHBJ010000000
VERSION     DAPHBJ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.268"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            264..1436
                     /gene="istA"
                     /locus_tag="RHZ76_004271"
     CDS             264..1436
                     /gene="istA"
                     /locus_tag="RHZ76_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HDV2814786.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKSMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRRFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1436..2233
                     /gene="istB"
                     /locus_tag="RHZ76_004272"
     CDS             1436..2233
                     /gene="istB"
                     /locus_tag="RHZ76_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HDV2814787.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVKSGQHQ"
     gene            complement(2393..2743)
                     /locus_tag="RHZ76_004273"
     CDS             complement(2393..2743)
                     /locus_tag="RHZ76_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814788.1"
                     /translation="MFTALNDKNTFGYPFEKIRNAIAVPSEKNVDAATSSGLEVLSRR
                     YDAFRQELDAAGELGNWEYDLDTYNHCIAVLQRYFTGNPSGLTERDARIYSHYLQTEH
                     KRFVKLAEELAAGR"
     gene            complement(2887..3348)
                     /locus_tag="RHZ76_004274"
     CDS             complement(2887..3348)
                     /locus_tag="RHZ76_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814789.1"
                     /translation="MARLLPFLKMDTVLIISSWPTGLYDYVQYMQMQSICIVIAYADG
                     KYMRMPHLCRCPAYGYVEPMLYSLFSRSDPKKGSSGSAFRPPARCGPATQDVAVFLAD
                     DDARCCVPPLPRDPARSSRKQCFAYFLTVVHFRKLRNNIKVGSGRNGHPVI"
     gene            3305..3535
                     /locus_tag="RHZ76_004275"
     CDS             3305..3535
                     /locus_tag="RHZ76_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDV2814790.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFAQFEKADPDFMAEREDVVSDEGRFNL"
     gene            3532..3948
                     /locus_tag="RHZ76_004276"
     CDS             3532..3948
                     /locus_tag="RHZ76_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HDV2814791.1"
                     /translation="MNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITY
                     SEMRFGATGPKASPRHVELVDAFCARLDAILPWDRAAVDATTEIKVALRMAGTPIGPN
                     DTAIAGHAIAAGAVLVTNNTREFERVPDLVLEDWVR"
     gene            complement(4046..4186)
                     /locus_tag="RHZ76_004277"
     CDS             complement(4046..4186)
                     /locus_tag="RHZ76_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004123533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV2814792.1"
                     /translation="MLAHTGLPVGTIAQTLGFEETTHFSKFFKRVADLTLGEFRLKQT
                     ES"
BASE COUNT         1044 a         1084 c         1169 g          933 t
ORIGIN      
        1 gtaaaaaaac cagctctgta ctaatgtaaa gacgctcgct ggccagcttc gctgctgcag
       61 ttctctcctc acgtctgagt gtgaagtggt gagtaaggaa caccgctccg acagcagatg
      121 cgatggtcga tacagctgta attatctgcg gccccagact ggtgtcagcg ccagtgatat
      181 aagacggtaa ttcaccattt ggattgtccg ctccacccaa catgttgttt ccttaaggtt
      241 ctcacaccag aaaggacatc aacatgctga gcagagagga cttttacatg ataaagcaaa
      301 tgcgccagca gggcgcgtac attgtcgata ttgcgactca gattggttgc tctgaacgga
      361 cggtcagacg ctacctcaaa taccctgaac cgccagccag aaagacccgc cacaaaatgg
      421 ttaagctgaa accgtttatg gattacatcg acatgcgcct ggcagagaat gtctggaata
      481 gcgaggttat cttcgcggag attaagtcaa tgggctatac gggcggacgt tccatgctgc
      541 gttactacat ccagcccaaa cgtaaaatgc gtccgtcaaa aagaacagtt cgcttcgaaa
      601 ctcagcctgg ataccagctc cagcatgact ggggcgaagt tgaggtggag gttgccgggc
      661 aacggtgcaa agttaacttt gcggttaata cgctggggtt ctcccgccgc ttccatgtct
      721 tcgccgcacc aaaacaggat gctgagcata cctacgaatc actggttcgc gccttccgct
      781 acttcggtgg ttgtgtgaaa acggtgctgg tcgataacca gaaggctgcg gtgctgaaaa
      841 ataacaacgg gaaagtcgtg ttcaactccg gattcctgtt gctggccgac cactataact
      901 tcctgccacg ggcatgccgt ccacgcaggg ccagaacaaa aggtaaggtt gagcggatgg
      961 tgaaatacct caaggagaac ttcttcgtcc ggtaccgcag gttcgacagc ttcactcatg
     1021 ttaatcaaca actggagcaa tggatagccg atgtggctga caaacgggaa cttcgccagt
     1081 tcaaagaaac gccggaacag cgcttcgcgc tggagcagga acatctgcag ccgttaccgg
     1141 atacggactt cgataccagt tacttcgata tccgccatgt gtcctgggac agctatatcg
     1201 aggttggtgg taatcgttac agcgttcccg aagcgctgtg tggtcagccg gtatcgatac
     1261 gaatatcgct ggatgacgag ttgcggatct acagtaatga gaaactggtg gcctcacatc
     1321 gcctctgttc ggcatcgtct ggctggcaga cagtgccgga gcatcacgcc ccgctctggc
     1381 agcaggtcag tcaggtggaa catcgaccac tgagtgccta tgaggagctg ttgtgatgca
     1441 tgagctggaa gtcctgctga gtcgcctgaa aatggagcat ctgagttatc acgttgaaag
     1501 cctgctggaa caggcagcta aaaaagagct gaactaccgg gagttcctgt gcatggcgct
     1561 acagcaggaa tggaacggca ggcatcagcg cggtatggag tccaggctga agcaggctcg
     1621 tctgccgtgg gtcaaaacgc tggagcagtt cgactttacc ttccagccgg gcatcgaccg
     1681 taaggttgtc cgggaactgg ctggtctggc gttcgtggag cgcagcgaaa acgtgatcct
     1741 gctgggacct cctggtgtcg gaaaaactca tctggccata gctcttggcg tgaaagcagt
     1801 ggatgcggga catcgggtac tgtttatgcc actggacaga ctgatcgcga cactgatgaa
     1861 agcgaaacag gaaaaccggc tggagcgtca gctgcagcaa ctgagttatg cccgggtgtt
     1921 gatcctggat gaaataggct atctgccgat gaacagagag gaagccagcc tgttcttccg
     1981 gctactgaac cgtcgatatg aaaaagcgag catcatactg acgtcaaaca aagggttcgc
     2041 agactgggga gaaatgttcg gagatcacgt gctggcaaca gcgatactgg atcggttgct
     2101 acatcactca accacgctga atatcaaagg agagagttac cggttaaaag agaaacgtaa
     2161 agctggagtg ctgaccaaaa acacaacgcc aatcagtgat gatgaaatgg tgaaaagcgg
     2221 acagcatcag taacgaaagt atcttagcgg gcatgaaaat ggcaaataac ggtcaaacat
     2281 cgtggcgttg acactggcgc tgtccttcac caccttcacg ccttcggtcg caataaacca
     2341 gtcagccatg tttattcctt tattgttgag aaatcccgcc ctctgtccgt agtcagcggc
     2401 cggcagccag ctcttcagcc agtttcacaa accttttgtg ctcagtctgc aggtaatggc
     2461 tgtaaatccg ggcatcacgt tctgtgagcc cggatggatt gccggtgaag taacgttgca
     2521 gcaccgcaat acagtgattg taggtatcga gatcgtattc ccagtttccc agctcaccgg
     2581 ccgcatccag ctcctggcga aaagcatcat acctccggct gaggacttca agaccggaac
     2641 tggtggcagc atcaacgttt ttttcactag gtacagcgat agcattacgg atcttctcaa
     2701 acgggtaacc gaaagtgttt ttatcgttca gggcagtaaa catgtccttc cctcagttga
     2761 atagttagcc gtgcaatttt accacacctc aatcatcgtc agatgagtgc cactctgtcg
     2821 cgacagtgac tttcacgaga gtgattttca catactaaaa taataagcag ccatataacc
     2881 acgtaattaa attaccggat ggccattccg gccagatccg acttttatgt tattccgcag
     2941 tttccggaag tgtaccacgg tcaggaagta agcgaagcac tgttttctgc tgctgcgcgc
     3001 cgggtcacgg ggcaggggag ggacacaaca tctagcgtca tcgtcagcca ggaacaccgc
     3061 tacatcttgt gttgccgggc cgcagcgcgc cggaggccgg aaagcagatc cggaactacc
     3121 ttttttcgga tctgaccggg aaaagagaga atacaacata ggttcaacat atccatatgc
     3181 cgggcatctg cataggtgtg gcatacgcat atacttacca tctgcatatg ctatgactat
     3241 acatatgctt tgcatctgca tatactgaac atagtcatac aggccggtcg gccaggagga
     3301 aatgatgaga actgtatcca tttttaaaaa cggcaataac cgcgccattc gtctgccccg
     3361 cgatctggat tttgagggag tgagcgagct ggagatcgtc cgggaagggg acagcattat
     3421 tttgcgtccc gtccggccga cctggggctc gttcgcgcag ttcgagaagg cagacccgga
     3481 ctttatggcg gagcgcgagg acgttgtcag cgacgaagga cgatttaacc tgtgaacaag
     3541 acctatatgc tcgacacctg tatctgctcg ttcatcatgc gcgagcagcc ggaagccgtc
     3601 ctgaagcgcc tggagcaggc ggtgctgcgc ggccagcgca tcgttgtctc ggccatcact
     3661 tattctgaaa tgcgcttcgg ggccaccggc ccgaaggcct ccccgcgcca cgtcgagctg
     3721 gtcgacgcgt tctgcgcccg cctcgatgcc atcctgccct gggatcgcgc tgcggtggac
     3781 gccaccacgg agattaaggt ggcgctgcga atggccggga cgccgatcgg cccgaacgac
     3841 acggccattg ccgggcacgc catcgccgcc ggtgccgtgc tggtgacgaa taatacgcgg
     3901 gagtttgagc gggtgccgga tctggtgctg gaagactggg taagataacg ttgtaaaaca
     3961 atgcggtgca ggtgaaaaat ccggtcagat accggaccag ggtgcagtct ctcctgtaca
     4021 gggcaatcac tctcaccggc cccgttcagc tctctgtctg cttcaggcgg aactcgccaa
     4081 gggtaaggtc tgcaacgcgt ttaaagaact ttgagaaatg tgtggtttct tcaaatccga
     4141 gcgtctgtgc aatggtccct acgggtaaac cggtatgcgc cagcattcgt tttgcttcaa
     4201 gaacgatccg ggcagctata aacgcttttg
//
LOCUS       DAPHBJ010000267         4228 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.269, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000267 DAPHBJ010000000
VERSION     DAPHBJ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.269"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            193..1125
                     /gene="rfaD"
                     /locus_tag="RHZ76_004278"
     CDS             193..1125
                     /gene="rfaD"
                     /locus_tag="RHZ76_004278"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HDV2814793.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1135..2181
                     /gene="rfaF"
                     /locus_tag="RHZ76_004279"
     CDS             1135..2181
                     /gene="rfaF"
                     /locus_tag="RHZ76_004279"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HDV2814794.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDIIPQR
                     VLEELNALLLQEEA"
     gene            2185..3177
                     /gene="rfaC"
                     /locus_tag="RHZ76_004280"
     CDS             2185..3177
                     /gene="rfaC"
                     /locus_tag="RHZ76_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HDV2814795.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRDLIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3174..>4228
                     /gene="rfaL"
                     /locus_tag="RHZ76_004281"
     CDS             3174..>4228
                     /gene="rfaL"
                     /locus_tag="RHZ76_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HDV2814796.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTCLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELIAYYKDYQQGVMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFAVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTANLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVVDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     ISLMMLYCAILKECIKNG"
BASE COUNT         1109 a          967 c         1042 g         1110 t
ORIGIN      
        1 tgatactaaa gcactattca cgtgcaaatc caacatccgc catgaaggac tggctaaaac
       61 ccaaactagt ttgttgcaat tagcatcctt gcatctctat gtaaagggct gaagggattc
      121 ggatgtgatg gtatgattac agacattcgt gtctgagatt gtctctgact ccataattcg
      181 aaggttacag ttatgatcat cgttaccggc ggcgcgggct ttatcggcag caacatcgtt
      241 aaagccctga atgacaaagg catcaccgat attctggtgg tggacaacct gaaagacggc
      301 accaagtttg tgaacctggt ggatctggat atcgcggact atatggataa ggaagacttc
      361 ctgatccaga ttatggctgg cgaagagttc ggcgatgtcg aagcgatttt ccacgaaggc
      421 gcgtgctctt ccaccaccga gtgggacggc aagtatatga tggataacaa ctatcaatac
      481 tccaaagagc tgctgcacta ctgcctggag cgcgaaatcc cgttcctgta cgcttcttct
      541 gcagccactt acggcggtcg cacctccgac tttattgaat cccgcgaata tgaaaaaccg
      601 ttgaatgtct acggttactc aaaattcctg tttgatgaat atgttcgcca aatcctgccg
      661 gaagccaact cacagattgt tggcttccgc tatttcaacg tttatggacc gcgtgaaggc
      721 cataaaggca gcatggcgag cgtcgctttc catctcaaca ctcagcttaa caacggtgaa
      781 tcgccgaagc tgttcgaagg tagcgagaac ttcaaacgcg acttcgttta cgtaggcgac
      841 gtggcagatg taaacctgtg gttcctggaa aacggcgttt ccggtatctt caacctcggc
      901 accggtcgtg cagaatcctt ccaggccgta gcagatgcta ctctggctta tcacaagaaa
      961 ggccagatcg aatacattcc gttcccggat aaactgaaag gccgctatca ggcgttcact
     1021 caggcagatc tgacaaatct gcgcgcggcg ggttacgaca aaccgttcaa aaccgttgct
     1081 gaaggtgtaa cggaatacat ggcctggctg aatcgcgacg cataagagct ctgcatgaaa
     1141 atactggtga tcggcccgtc ttgggttggc gacatgatga tgtcgcaaag tctctatcgc
     1201 acgctccagg cgcgctatcc ccaggcgata atcgacgtga tggcaccggc atggtgccgt
     1261 ccattgttat cgcggatgcc ggaagttaac gaagctatcc ctatgcctct cggtcatgga
     1321 gcgctggaaa tcggcgaacg ccgcaaactg ggtcatagcc tgcgtgaaaa gcgctacgac
     1381 cgcgcctacg tcttacccaa ctccttcaag tccgcattag tgcctttctt cgcgggtatt
     1441 cctcatcgca ccggctggcg cggcgagatg cgctacggtt tacttaacga tgtacgcgtg
     1501 ctcgataaag aagcctggcc gctaatggtg gaacgctatg tcgcgctggc ctatgacaaa
     1561 ggcattatgc gtaccgcaca agatctgccg cagccattgt tatggccgca gttgcaggtg
     1621 agcgaaggtg aaaaatcata tacctgtaat caattttcgc tttcatcaga acgtccgatg
     1681 attggctttt gccccggagc ggagtttggt ccggcaaaac gctggccaca ctaccactat
     1741 gcggaactgg caaagcagct gattgatgaa ggttatcaag tggttctgtt tggctcggcg
     1801 aaagatcatg aagcgggcaa tgagattctt gccgctttga ataccgagca gcaggcatgg
     1861 tgtcgtaacc tggcggggga aacacagctt gatcaagcgg ttatcctgat tgcagcctgt
     1921 aaagccattg tcactaacga ttctggcctg atgcacgttg cggcggcgct caatcgtccg
     1981 ctggttgccc tgtatggtcc gagtagcccg gacttcacac cgccgctatc ccataaagcg
     2041 cgcgtgatcc gcctgattac cggctatcac aaagtgcgta aaggtgatgc tgcggagggt
     2101 tatcaccaga gcttgatcga cattattccc cagcgcgtac tggaagaact caacgcgcta
     2161 ttgttacaag aggaagcctg acggatgcgg gttttgatcg ttaaaacatc gtcgatgggc
     2221 gatgttctcc atacgttgcc cgcactcact gatgcccagc aggcaatccc ggggattaag
     2281 tttgactggg tggtggaaga agggttcgca caaattcctt cctggcacgc cgctgttgag
     2341 cgagttattc ctgtggcaat acgtcgctgg cgtaaagcct ggttctcggc ccccataaaa
     2401 gctgaacgga aagcgtttcg tgaagcgcta caagcagaga actatgacgc agttatcgac
     2461 gctcaggggc tggtaaaaag cgcggcgctg gtgacgcgtc tggcacatgg cgtaaagcat
     2521 ggaatggact ggcaaaccgc tcgcgaacca ctggcaagct tgttttacaa tcgtaagcat
     2581 catattgcaa aacagcagca cgccgtagaa cgcacccgcg aactgtttgc caaaagtctg
     2641 ggctatagca aacctcaaac ccagggcgat tatgctattg cacagcattt cctgacgaac
     2701 ctgcctacag atgctggcga atatgccgta tttcttcatg cgacgacccg tgatgataaa
     2761 cactggccgg aagaacactg gcgagatttg attggtttac tggcagattc aggaatacgg
     2821 attaaacttc cgtggggcgc gccgcatgag gaagaacggg cgaaacgact ggcggaagga
     2881 tttgcttatg ttgaagtatt gccgaagatg agtctggaag gcgttgcccg cgtgctggcc
     2941 ggggctaaat ttgtagtgtc ggtggatacg gggttaagcc atttaacggc ggcactggat
     3001 agacccaata tcacggttta tggacctacc gatccgggat taattggcgg gtatgggaag
     3061 aatcaagtag agtgtcgttc tacaagtatg tctcttgcag atttgccagc tcaaaccgtt
     3121 tttcaaaatt taaatcttga aataataacc aataagttga catcggagat aagatgacct
     3181 caacattatt tttctctctc gagaaaaaaa actggatagc gtactggaac agagctctcg
     3241 tattcttatt cattaccacc tattttttgg gtgggattac aaggtataaa catcttattg
     3301 ttattcttat gacaataacg acaatcgtct atctctgcaa acggccaaaa cactatcttt
     3361 cactatttaa aacatgtctt tttggtagtg ttgccatatt aactattgct gcattgctat
     3421 cacttcttca atccccagat gcaggtgcta gcatgaagga aattttcaaa gctatcattg
     3481 aaaatacttt actatgcaca atagcaatac cggtcatatt gagagacgag aaaagagaag
     3541 atgtcgaaaa aatcgttttc ttctcattta taagtgcgtt gggcttacgt tgtttttctg
     3601 aattgattgc ctattataag gactatcaac aaggggtaat gccatttgca gattatagac
     3661 accgtagcat ttctgactcg atggtctttt tatttcctgc attgttaaat ctctggctta
     3721 tcaaatcagc aaaataccgc atttcttttg cggttctaag cgttattttt atttttctga
     3781 tattaggaac tttatccaga ggagcctggc tttccgtgtt agtcattgga ttaatatgga
     3841 ttctgatgtt taaacaatgg aagttactat tagtaggagt aatggtttcc atcattgcat
     3901 tatcggttat tttcacacat aaggagatga ccgcaaatct aacgtataaa cttcaacaaa
     3961 ctaatagttc ttatcgctat gcaaatggta ctcaaggcag cgcactcgat ctaatattag
     4021 aaaatcctgt tattggttac ggttacggta acgttgcata taaagatgtc tataataaac
     4081 gtgtcgttga ttatccagaa tggaccttta gacaatcgat agggccacat aattttgcgc
     4141 tattcatctg gtttggcact ggtttattag ggctgataag tcttatgatg ctatactgcg
     4201 caatattgaa agagtgtata aaaaatgg
//
LOCUS       DAPHBJ010000268         4153 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.270, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000268 DAPHBJ010000000
VERSION     DAPHBJ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.270"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(52..309)
                     /locus_tag="RHZ76_004282"
     CDS             complement(52..309)
                     /locus_tag="RHZ76_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814797.1"
                     /translation="MQKPLPVRWLIIQGLTENEHCGIQAYACNTESQEDLYAEVPLVG
                     RLTGIEEVKVEAIAMSKLHNMSCPPYMGHSAAVIFHLIYLS"
     gene            432..2630
                     /gene="speF"
                     /locus_tag="RHZ76_004283"
                     /pseudo
     CDS             432..2630
                     /gene="speF"
                     /locus_tag="RHZ76_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            2627..3946
                     /gene="potE"
                     /locus_tag="RHZ76_004284"
     CDS             2627..3946
                     /gene="potE"
                     /locus_tag="RHZ76_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HDV2814798.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            4022..4153
                     /locus_tag="RHZ76_004285"
                     /pseudo
     CDS             4022..4153
                     /locus_tag="RHZ76_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="response regulator in two-component regulatory
                     system with KdpD; regulates the kdp operon involved in
                     potassium transport; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
BASE COUNT         1004 a          976 c         1047 g         1126 t
ORIGIN      
        1 ttcacagttc aaccgtattt cctggataga atatcttcac cttcattcac atcaggaaag
       61 gtaaattaaa tggaaaataa cagccgcact atgccccata taaggcggac aactcatatt
      121 atgaagtttg ctcatcgcaa tagcttcgac tttcacttct tcaatgcccg ttagtctacc
      181 gactaagggc acttcagcgt acaggtcttc ctgactctct gtattacagg catatgcctg
      241 tattccgcag tgctcattct ctgttaaccc ttgtattatc agccacctga ctggtaaggg
      301 tttctgcatt ctgaaaaaag caatctacct gatttattta tcagcggggt aactttgctt
      361 ttttccggca cgatcgattt ctcattcgag aaattgagga cctgctatta cctaaaataa
      421 agagatgaaa aatgtcaaaa ttaaaaattg cggttagtga ttcttgcccg gactgtttta
      481 ccacgcagcg agaatgtatc tacattaatg aaagtcgtaa tatcgatgtg gcggcaatag
      541 ttttatcgct caacgatgtt acatgcggaa aactcgatga aatcgatgcc acgggttaag
      601 gcatcccggt atttattgct actgaaaatc aagaacgtgt acccgcagag tatttgcccc
      661 gtatttcggg tgtctttgag aattgcgaat cgcgacgaga attttatggt cgccagttag
      721 aaaccgctgc cagccattat gaaactcaac tgcgcccacc tttcttccgc gcactggtcg
      781 attatgtcaa tcaaggtaac agcgcgtttg attgccctgg tcatcagggc ggcgaatttt
      841 tccgtcgcca tccggcgggg aatcagtttg tggaatactt tggtgaggcg ctgttccgtg
      901 ccgacttgtg caacgccgac gtagcgatgg gcgatctgct gattcacgaa ggcgcgccat
      961 gcattgcaca gcaacatgcg gcaaaagtgt ttaatgccga taaaacctac ttcgttttaa
     1021 atggcacttc atcttctaac aaagtggttt taaacgccct gctaacaccg ggtgatctgg
     1081 tgctgtttga tcgcaataac cacaaatcta accaccacgg agcgttgcta caggctggtg
     1141 caacaccggt ttatctggaa acggcacgta acccgtatgg ctttatcggt ggcattgatg
     1201 cgcactgttt tgaagaaagt tacctgcgtg agctgatcgc ggaagtcgca ccgcagcggg
     1261 caaaagaggc tcgtcctttc cgcctcgctg tgattcagtt aggcacctac gacggtacga
     1321 tttataacgc ccgccaagtg gtggataaaa ttggtcatct gtgtgactac atcctgtttg
     1381 actcagcatg ggtcggctat gaacagttta ttccgatgat ggcggactgt tcgccgctgt
     1441 tgctggatct taatgagaac gatccgggta ttctggttac gcaatctgtg cataaacaac
     1501 aggctggttt ttctcagact tcacaaattc ataaaaaaga cagccacatc aaagggcaac
     1561 agcgttatgt accgcacaaa cgcatgaaca acgcctttat gatgcacgcc tccaccagcc
     1621 cgttctatcc gctgtttgcc gcactggata tcaacgccaa aatgcatgaa ggtgtcagcg
     1681 gtcgtaatat gtggatggat tgtgtggtaa atggcattaa tgcccgcaaa ctgatcctcg
     1741 ataactgtca gcatattcgt ccgttcgtac ctgaactggt ggatggtaaa ccctggcagt
     1801 cgtatgaaac agcgcaaatt gcggttgatc tgcgcttctt ccagtttgta ccaggggaac
     1861 actggcattc ttttgaaggc tatgcagaga atcaatactt tgtcgatcca tgcaaactgt
     1921 tgctgacaac cccaggtatt gatgcacgta acggcgaata tgaagcgttc ggtgtacccg
     1981 cgacgattct tgctaacttc ctgcgcgaaa atggcgtagt gccggaaaaa tgcgatctta
     2041 actccatcct cttcctgctg actccggcag aagatatggc caaacttcag caacttgttg
     2101 ccctgctggt acgcttcgaa aaactgcttg agtccgacgc gccattagca gaagtgctac
     2161 cttccatcta caaacagcat gaagagcgct acgccggtta taccctgcgt cagttgtgtc
     2221 aggaaatgca tgatttgtat gcccgccaca acgtgaaaca actgcaaaaa gagatgttcc
     2281 gtaaggagca cttcccacgc gtcagcatga atccgcaaga agccaactac gcctatttac
     2341 gcggtgaagt ggaactggtt cgtctgccgg atgcagaagg ccgtatcgct gccgaaggtg
     2401 cgcttcctta tcctccgggt gtgctgtgtg ttgttccggg tgaaatctgg ggtggtgctg
     2461 ttctgcgtta cttcagcgct ctggaagaag ggatcaacct gctgccaggt tttgcaccgg
     2521 agctgcaggg tgtctatatc gaagaacatg atggtcgtaa gcaagtttgg tgctatgtca
     2581 tcaagcctcg tgatgcgcaa agcaccctgt tgaaagggga aaaattatga gtcaggctaa
     2641 atcgaacaaa atgggcgtcg ttcagttaac catactgacg atggtcaaca tgatgggctc
     2701 cggtatcatc atgctgccga caaagcttgc cgaagtcggg acaatctcaa ttatctcctg
     2761 gctggtgaca gccgtaggct caatggcact ggcatgggca ttcgcaaaat gcggtatgtt
     2821 cagccgtaaa tcaggcggta tgggcggtta tgccgagtat gctttcggta aatccggtaa
     2881 ctttatggcg aactatacct acggcgtctc actgctgatt gctaacgtcg cgattgctat
     2941 ttcggcggtt ggttacggca ccgaactgct cggcgcgagt ttgtcgccag tgcagattgg
     3001 tcttgcgacc atcggggtgc tgtggatttg taccgtggct aactttggtg gtgcgcgcat
     3061 taccgggcaa atcagtagca ttaccgtgtg gggggtcatt attccggtcg tcggtctgtg
     3121 cattattggc tggttctggt ttagcccgac gctgtacgtt gattcctgga atccgcatca
     3181 tgcaccgttc ttcagtgcgg taggttcttc catcgctatg acgctgtggg cttttcttgg
     3241 tctggagtct gcgtgtgcga atactgatgt agtggaaaac ccggaacgta atgtgccaat
     3301 cgcggtactc ggcggtacgt taggtgcggc ggtgatttat atcgtctcca ccaacgtgat
     3361 tgccgggatt gtgccaaata tggagctggc aaattcaacg gcaccatttg gtctggcctt
     3421 cgcgcagatg ttcacgccgg aagtgggtaa agtcattatg gcgctgatgg tgatgtcctg
     3481 ctgcggttcg ctacttggct ggcagttcac cattgcccag gtgtttaaat cttcatctga
     3541 tgaaggctac ttccctaaaa ttttctcccg tgtaaccaaa gtggatgcac cggtgcaggg
     3601 aatgttgacc attgtgatta ttcagagtgg attggcactg atgaccatta gcccgtcgct
     3661 aaacagtcag ttcaacgtgc tggttaacct ggccgtggtg accaatatca ttccgtatat
     3721 tctgtcaatg gcggcattag tcattattca gaaggtcgct aatgtgcccc cctcaaaagc
     3781 gaaagttgca aactttgttg cttttgttgg cgcgatgtat agcttttatg cgctgtactc
     3841 atccggggaa gaagccatgc tgtacggttc catcgtgacc ttccttggtt ggacactgta
     3901 tggtctggta tcaccacgct ttgaactgaa aaataaacac ggttaataaa acaaagtaaa
     3961 aaagggcgat tgaatgatcg ccctttctgt ttgttggcaa atagttcgtt atttatgccg
     4021 catccggcag atgtagggtc taaacgcgat cgaacacagc aactatttgc gtaattatat
     4081 ggggcatctg cgacaaaaac tggaacagga tccggcccgg cctcgccatt ttattactga
     4141 aaccggtatt ggg
//
LOCUS       DAPHBJ010000269         4120 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.271, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000269 DAPHBJ010000000
VERSION     DAPHBJ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.271"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            146..1249
                     /gene="proB"
                     /locus_tag="RHZ76_004286"
     CDS             146..1249
                     /gene="proB"
                     /locus_tag="RHZ76_004286"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDV2814799.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1261..2514
                     /gene="proA"
                     /locus_tag="RHZ76_004287"
     CDS             1261..2514
                     /gene="proA"
                     /locus_tag="RHZ76_004287"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDV2814800.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADALANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2629..2704
                     /locus_tag="RHZ76_004288"
     tRNA            2629..2704
                     /locus_tag="RHZ76_004288"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2662..2664,aa:Thr,seq:cgt)
     gene            2868..4064
                     /locus_tag="RHZ76_004289"
     CDS             2868..4064
                     /locus_tag="RHZ76_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV2814801.1"
                     /translation="MKLNARQVDAAKPREKAYKLADGAGLYLEVVPSGSRYWRMKYRF
                     NGKEKRMAFGVYPAVSLAQARALRDEAKKKLAEGIDPSFAKKEEKLVRDVQLNNTFQS
                     VALEWHGTKVSRWSEGYASDIIEAFNKDIFPYIGQQPVNEIKPLVLLNVLRRMESRGA
                     TEKAKKVRQRCSEVFRYAIVTGRAEYNPAADLTSAMSGHESKHYPFLTVEELPDFFKA
                     LSGYTGSPLVVLAARLLILTGVRTGELRGAFWSEFDLEKAVWEIPADRMKMKRPHLVP
                     LSTQALEIVQQLKVMSGQYPLVFPGRNDPRKTMSEASINQVFKRIGYTGKVTGHGFRH
                     TMSTILHEEGFNTAWIETQLAHVDKNAIRGTYNHAQYLEGRREMMQWYADYINNIGKN
                     KLIIAL"
BASE COUNT         1007 a         1004 c         1157 g          952 t
ORIGIN      
        1 taaaagcgta aacaactgcc gctaggcttg ctgatcccgc gcaacaaaac gccatgcttt
       61 gctcgcagat ggttggcaac cgacgacagt cctgctaaaa cgttcgtttg atatcatttt
      121 tcctaaaatt gaatggcaga gaatcatgag tgacagccag acgctggtgg taaaactcgg
      181 caccagtgtg ctaacaggcg gatcgcgccg tctgaaccgt gcccatatcg ttgaacttgt
      241 tcgccagtgc gcgcagttac atgccgccgg gcatcggatt gttattgtga cgtcgggcgc
      301 gatcgccgcc ggacgtgagc acctgggtta cccggaactg ccagcgacca tcgcctcgaa
      361 acaactgctg gcggcggtag ggcagagtcg actgattcaa ctgtgggaac agctgttttc
      421 gatttatggc attcacgtcg ggcaaatgct gctgacccgt gctgatatgg aagaccgaga
      481 acgcttcctg aacgcccgcg acaccctgcg tgcgttgctc gataacaata tcgttccggt
      541 aatcaatgag aacgatgctg tcgctacggc agagattaag gtcggcgata acgataacct
      601 ttctgcgctg gcggcgattc tggcgggtgc cgataaactg ttgctgctga ccgatcaaaa
      661 aggtttgtat accgctgacc cgcgcagcaa tccgcaggca gaactgatta aagatgttta
      721 cggcattgat gacgcactgc gcgcgattgc cggtgacagc gtttcaggcc tcggaactgg
      781 cggcatgagt accaaattgc aggccgctga cgtggcttgc cgtgcgggta tcgacaccat
      841 tattgccgcg ggcagcaagc cgggcgttat tggtgatgtg atggaaggca tttccgtcgg
      901 tacgctgttc catgcccagg cgactccgct tgaaaaccgt aaacgctgga ttttcggtgc
      961 gccgccggcg ggtgaaatca cggtagatga aggggcaact gccgccattc tggaacgcgg
     1021 cagctccctg ttgccgaaag gcattaaaag cgtgactggc aatttctcgc gtggtgaagt
     1081 catccgcatt tgcaacctcg aaggccgcga tatcgcccac ggcgtcagtc gttacaacag
     1141 cgatgcatta cgccgtattg ccggacacca ctcgcaagaa attgatgcaa tactgggata
     1201 tgaatacggc ccggttgccg ttcaccgtga tgacatgatt acccgttaag gagcaggctg
     1261 atgctggaac aaatgggcat tgccgcgaag caagcctcgt ataaattagc gcaactctcc
     1321 agccgcgaaa aaaatcgcgt gctggaaaaa atcgccgatg aactggaagc acaaagcgaa
     1381 atcatcctca acgctaacgc ccaggatgtt gctgacgcgc tagccaatgg ccttagcgaa
     1441 gcgatgcttg accgtctggc actgacgccc gcacggctga aaggcattgc cgacgatgta
     1501 cgtcaggtgt gcaacctcgc cgatccggtg gggcaggtaa tcgatggcgg cgtactggac
     1561 agcggcctgc gtcttgagcg tcgtcgcgta ccgctggggg ttattggcgt gatttatgaa
     1621 gcgcgcccga acgtgacggt tgatgtcgct tcgctgtgcc tgaaaaccgg taatgcggtg
     1681 atcctgcgcg gtggcaaaga aacgtgtcgc actaacgctg caacggtggc ggtgattcag
     1741 gacgccctga aatcctgcgg cttaccggcg ggtgccgtgc aggcgattga taatcctgac
     1801 cgtgcgctgg tcagtgaaat gctgcgtatg gataaataca tcgacatgct gatcccgcgt
     1861 ggtggcgctg gtttgcataa actgtgccgt gaacagtcga caatcccggt gatcacaggt
     1921 ggtataggcg tatgccatat ttacgttgat gaaagtgtag agatcgctga agcattaaaa
     1981 gtgatcgtca acgcgaaaac tcagcgtccg agcacatgta atacggttga aacgttgctg
     2041 gtgaataaaa acatcgccga tagcttcctg cccgcattaa gcaaacaaat ggcggaaagc
     2101 ggcgtgacat tacacgcaga tgcagctgca ctggcgcagt tgcaggcagg ccctgcgaag
     2161 gtggttgctg ttaaagccga agagtatgac gatgagtttc tgtcattaga tttgaacgtc
     2221 aaaatcgtca gcgatcttga cgatgccatc gcccatattc gtgaacacgg cacacaacac
     2281 tccgatgcga tcctgacccg cgatatgcgc aacgcccagc gttttgttaa cgaagtggat
     2341 tcgtccgctg tttacgttaa cgcctctacg cgttttaccg acggcggcca gtttggtctg
     2401 ggtgcggaag tggcggtaag cacacaaaaa ctccacgcgc gtggcccaat ggggctggaa
     2461 gcactgacca cttacaagtg gatcggcatt ggtgattaca ccattcgtgc gtaaataaaa
     2521 ccgggtgatg caaaagtagc catttgattc acaaggccat tgacgcatcg cccggttagt
     2581 tttaaccttg tccaccgtga ttcacgttcg tgaacatgtc ctttcagggc cgatatagct
     2641 cagttggtag agcagcgcat tcgtaatgcg aaggtcgtag gttcgactcc tattatcggc
     2701 accatttcaa actcttctca agtctaccaa aatcaactta aagcccgtat actgcggttt
     2761 ctagcccgta tcttatctcc tgttatcaac tggactcaac cggaatcaag ttacagttgg
     2821 gggcataagt gggggcattc tgtgttcggt ccagggagat gcccccaatg aagctaaatg
     2881 cacgacaggt agacgctgcg aaacccagag agaaagccta taagcttgca gatggtgctg
     2941 gtttgtatct tgaggttgtt ccttctggtt ctcgatactg gcggatgaaa tatcgcttca
     3001 atggaaaaga gaagcgtatg gcttttgggg tctatccggc agtgtccctt gcacaagcga
     3061 gggcactgcg tgatgaagcc aagaaaaagc tggctgaggg tatcgatcca tcatttgcta
     3121 agaaagaaga aaagctggtt cgggatgtgc agctcaacaa cacgttccag tctgtggcgc
     3181 ttgaatggca cggaacgaag gtaagccgat ggtcagaagg ttatgcctcc gacattatcg
     3241 aagctttcaa taaagatatt ttcccctata tcggccagca gccggtgaat gaaatcaaac
     3301 cgctggttct gcttaatgtg ctgcgtcgaa tggaaagccg tggcgcgaca gagaaggcca
     3361 agaaggttcg ccagcgttgc agtgaagtct ttcgttacgc catcgtgact ggtcgtgcgg
     3421 aatataaccc tgcagcggat cttaccagcg cgatgtcagg gcatgaatcg aagcattatc
     3481 ccttccttac cgttgaggag ttacctgatt tctttaaagc tctctctggt tatacaggca
     3541 gcccgctggt tgttcttgcc gcacgtttgc tgatccttac gggagttcgc accggcgagc
     3601 ttcgaggtgc tttctggagt gagtttgacc ttgagaaagc ggtgtgggaa attcctgccg
     3661 accgaatgaa gatgaaacgg cctcaccttg tccccctctc tacccaagca ctagagatcg
     3721 tacagcagct caaagtgatg tctgggcaat atcctctggt gtttcctggg aggaatgatc
     3781 cccgcaaaac gatgagcgaa gcgagtatta atcaggtgtt taagcggatt ggatacacgg
     3841 ggaaggtaac ggggcatggt ttccgtcaca cgatgagtac gattttgcat gaggaagggt
     3901 tcaatacggc gtggattgaa acccagctgg ctcacgttga taagaatgcg attcgtggga
     3961 cgtataacca tgctcagtat ctggaagggc gtagggagat gatgcagtgg tatgccgact
     4021 atattaataa cattggcaaa aataaactta tcatagcctt atgatactag ctccgaattt
     4081 agccgtttat tggtagataa cgtgaaaact caagcagagt
//
LOCUS       DAPHBJ010000270         4103 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.272, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000270 DAPHBJ010000000
VERSION     DAPHBJ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.272"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..78
                     /gene="ldrD"
                     /locus_tag="RHZ76_004290"
     CDS             <1..78
                     /gene="ldrD"
                     /locus_tag="RHZ76_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HDV2814802.1"
                     /translation="WHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(165..1844)
                     /gene="bcsG"
                     /locus_tag="RHZ76_004291"
                     /pseudo
     CDS             complement(165..1844)
                     /gene="bcsG"
                     /locus_tag="RHZ76_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417995.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
     gene            complement(1841..2032)
                     /gene="bcsF"
                     /locus_tag="RHZ76_004292"
     CDS             complement(1841..2032)
                     /gene="bcsF"
                     /locus_tag="RHZ76_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HDV2814803.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2029..3600)
                     /gene="bcsE"
                     /locus_tag="RHZ76_004293"
     CDS             complement(2029..3600)
                     /gene="bcsE"
                     /locus_tag="RHZ76_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HDV2814804.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3873..4061
                     /locus_tag="RHZ76_004294"
     CDS             3873..4061
                     /locus_tag="RHZ76_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HDV2814805.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
BASE COUNT         1060 a         1004 c         1046 g          993 t
ORIGIN      
        1 tggcatgatt tagcggctcc ggtcattgct ggcattcttg ccagtatgat cgtgaactgg
       61 ctgaacaagc ggaagtaacg tgtcatgcgg gcgtcaggct gccgtaatgg caatttgcgc
      121 ccggaccagg ccgcaggggg gaaactctgc ggcctttttc gttcttactg cgggtaaggc
      181 acccagtcgc cgccgttcag gcgaacgtac ggtttatcct ggtattgaat aactactgca
      241 tttgagttct cggagaccgg tgctgtttgt ggcaacccac tggtgagttt tttccagtca
      301 acattgtctt cggtgaaaat cttgccatcg agaacgcgaa ccaccagatc ggagatagcc
      361 aggaagctgc tcggttgttc gatgacaatc ggtgccccct gatgcggtgc cttcatgccg
      421 aagaatttca ccccaacggg gacgtcggtg atagacgggc tagggatatc acgtaggcca
      481 gatacctgca ttctgtcgcc cttcagcgcg ccgccgtgtt ccggcaccac gaccaccatc
      541 actttacgac ccgatttctc aagttcagta aagaaggcgt ccagttcatc aaagaatttc
      601 tgcgcccgcg ctttgtaatc cgctgttttg ctgacccccg gataatggtt gccgtcatgc
      661 agtggaagcg tgttgtagaa cgtggcacta cggctgtttt tatctttttc ggtaacgtcc
      721 agccagcggt taagcacagc ggtatcgtca taaaccggcg aaccatcaaa gcccagcaaa
      781 ataaccggca gatttgtttg atccatcaat tcgctctgca tgccgccatt ttcgcgaact
      841 tctttcaaaa aaccgccgaa ctggccgtta tgtcccatca tcaggtgctg ggtaaagccc
      901 agtttcgaaa ggttatcaaa cagatagcag tcgttatttg ccggttgata cagattagtg
      961 tgcgaagtct gcccgcagct ggcgcgcagt aaacggatcg ccgccgggcc actgtaggag
     1021 gtggcggagt taaagttctt gaactcaata tcgaaatgcg accacagtgg atgcgacatc
     1081 aacccggcgg cttctatatc cgaccaggaa agcgaacaga tgttaatcac cagtagttca
     1141 aatggctgag catcagcggg cagcgaagac gggaaggtcg atttacgttt cgcctccgcg
     1201 ttatagaaat tattcagcca ggcgttaagg ttcgccgttg ttggcggtgc agtttgtgcg
     1261 ggcatatcac ccactaccgg tgcgccaccc gtcgccgcaa cggttgccgc tgcgttacca
     1321 cccgtcgttg ttacagtggt cgtcggttgt ccggctggtc acaaggagaa acttggtccc
     1381 gccagggtaa gtacgttcag ccatagcagt atggcaacca caaaaacggt aatgcgaatc
     1441 cattgtgaca ggaataacca ggccactaat aaaacaaaaa tggccccaat catctgccag
     1501 ttaataaagc gtgtgacaag gtcgattaaa taatcggtac tgaaccccgc cacctgcgaa
     1561 ccctggctca ttatgctttc cgggccaggc aaccaggtgt catgccagaa caaagcaaag
     1621 ccgatcggca gggcaatcca gtggcgcaag cgatgcaggc tgtagcgcgg aaggggcatc
     1681 agcagaaacg cggcaaacac caaattgagg agcggatgga agttaagata tcccgcccac
     1741 aacaggccga acttaaccag aaaatagaag ttccagccgg aaaggccgcg ccagtattgc
     1801 cagagggaag aaggcatggc ggtattttgc gtaaattgag tcattttttg gttgccctgg
     1861 ctttttccgt gcggcgtaac gtcccggccg gtttaacata acgaggttta gcaaagaaca
     1921 aacgtaaggt gtcgcgaatg cgtcgcaaag agtgccgcgc cagatagccc agcgggaaaa
     1981 atatcagtgc gcaaacgaca ataatttcaa tgatatcgct gatggtcatc atgatgagcg
     2041 ctccacagca tcatctaaca gtcgcatggg ttctggtatt cgtcgccagt ggcgaccatc
     2101 gtgctcggca ttgatgaccg gtttagaact ttgcgttaaa ggcagcggca tgccccattg
     2161 ttctggggca agcaagcgca tctgcaccag ctcggcactg atttgatcat cttcaaacca
     2221 gaccatacgg tttgagaaaa tgtcgccagt aggcaatggg aaaatatgat tcaacgcggt
     2281 atccagatcg ttaatccgac agaatgagag aaacagcacc agccgattac cgccaatggt
     2341 catgatatcg ccggtacggt tagggcgaca cagcgtcagg gcttgttcaa cgcggatacc
     2401 cggtaccgga cgtagggcaa ccagaacgcc tttaccgtgg gcaggtaata gagggttatt
     2461 catcatgttg ttgacggcat tacagaacac atcccacttc tggaaaccac gcagtttgag
     2521 cggctgggtc attgacagca aggtagtgat atcttccggc acatagcgac taaacttctg
     2581 cccttgcacg ctttcgatca tcgtcagaca acgggagagt ggcgcattcc acggaataac
     2641 catatttgca ccgcaggcca ataacaaacg ttcatcggtg gcgcgcaggc tagcggtatt
     2701 ttcccgcacg aggattttca tcgcactacc gcgctggcga cgcagggtat gaatgctgcg
     2761 ggccagtggc tcgatttgcg cattttgctg taaagaaaag accaccgtcg ccgcctgagc
     2821 ggtacgggct tcattgaaca ggacttcgtt attgttgaac agttgccagt gttccgatag
     2881 cggcggcgca ccttccagta cagcaacatt actcagaatg cgtttttcgt cgctgcgtgg
     2941 ttggatctcc gcctcttcgc tttgaactaa tgtccagata ccattttgtt gctgaacgct
     3001 aagctgctga cgggcgctga cccctttttc gttgcaccag aaggcaatat ccagcaaatg
     3061 ttggtcaccc tgaaaacgca aactggcaag accaaaaagt gaacggtatt cctcaagcaa
     3121 caatgaaaat tgtttatcgt tattatttcc gggattaatt accaaaagcg aacaatggtt
     3181 taacctgctc catttattca ttttatccaa ccatgagcga agccgctcgg caggaatgtt
     3241 ttgccatgcg ttatttgcgc aaacaagaat aaaaaggtaa ttatggggat caatagaaca
     3301 ctgcaaatca cgggtcaaat agtatagacc tttttcatga tttagcattg aaaataattt
     3361 tattttttcc ggcccttgag aatcatctaa ttgaaagatt ttcgccggat cgctgtccat
     3421 gctaatgacc gcgacgtgtg cggtttcagc ctgggatgca attgtttgat tcgccagact
     3481 gatagcatct tcatggcgat cgacgttaaa ccaccagacg ccgcctgctg gcatatgtcg
     3541 cagctcatcc cataatgatg agataccgat agagaataca gggtccacaa tgtccctcat
     3601 gtcaccattt tgtctgatta tcagtttact agcgaaagct aagaaataaa cctaccattg
     3661 aaattaaaac ttatcagatt tagcatgtga atcaatttag aaggtcacaa gacgtgtgat
     3721 tttagtcttt tcgtttttaa ctttttctgt tgcgtaacgg aaagtcaaaa agtgagcaaa
     3781 ttcccgtctc gccgtaaaca ataaaccggc gaaatgctca cggttagaat tgtctcatga
     3841 acggtacggt tatttcatag ggatcaagca atatgaataa caatgaacca gatactctgc
     3901 ctgatcccgc gataggctat atcttccaga atgatattgt ggcgttaaag caggcatttt
     3961 cactgcctga tattgattat gccgatattt cccaacgcga acagttggcc gcggcattaa
     4021 aacgctggcc gttgctggca gagtttgcgc aacaaaagta ggggattggt gaatggccgt
     4081 actgggattg cagggggtgc ggg
//
LOCUS       DAPHBJ010000271         4075 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.273, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000271 DAPHBJ010000000
VERSION     DAPHBJ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.273"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            79..1185
                     /gene="ompC"
                     /locus_tag="RHZ76_004295"
     CDS             79..1185
                     /gene="ompC"
                     /locus_tag="RHZ76_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDV2814806.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGETQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRDALRQNGDGVGGSITYDYEGFGIGAAVSSS
                     KRTDAQNTAAYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIGTRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1297..2352
                     /gene="apbE"
                     /locus_tag="RHZ76_004296"
     CDS             1297..2352
                     /gene="apbE"
                     /locus_tag="RHZ76_004296"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HDV2814807.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENEVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2426..3490
                     /gene="ada"
                     /locus_tag="RHZ76_004297"
     CDS             2426..3490
                     /gene="ada"
                     /locus_tag="RHZ76_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HDV2814808.1"
                     /translation="MKKATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADHVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3490..>4075
                     /gene="alkB"
                     /locus_tag="RHZ76_004298"
     CDS             3490..>4075
                     /gene="alkB"
                     /locus_tag="RHZ76_004298"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HDV2814809.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAG"
BASE COUNT          993 a         1061 c         1096 g          925 t
ORIGIN      
        1 ccgactgatt aatgagggtt aatcagtatg cagtggcata aaaaagcaaa taaaggcata
       61 taacagaggg ttaataacat gaaagttaaa gtactgtccc tcctggtccc agctctgctg
      121 gtagcaggcg cagcaaacgc tgctgaagtt tacaacaaag acggcaacaa attagatctg
      181 tacggtaaag tagacggcct gcactatttc tctgacaaca agtcagaaga cggcgaccag
      241 acctatgtac gtcttggttt caaaggcgaa actcaggtta ctgaccagct gaccggttac
      301 ggccagtggg aatatcagat ccagggcaat acctctgaag acaacaaaga aaactcctgg
      361 acccgtgtgg cattcgcagg tctgaaattc caggatgtgg gttctttcga ctacggtcgt
      421 aactacggcg ttgtttacga cgtaacttcc tggaccgacg tactgccaga attcggtggc
      481 gacacctacg gttctgacaa cttcatgcag cagcgtggta acggctttgc gacctaccgt
      541 aacactgact tcttcggtct ggttgacggc ctgaactttg ctgttcagta ccagggcaaa
      601 aacggtagcg taagcggcga aggcatgacc aacaatggtc gcgatgctct gcgtcagaac
      661 ggcgacggcg ttggcggttc tatcacttat gattacgaag gcttcggtat cggtgctgca
      721 gtttccagct ccaaacgtac tgatgctcag aacaccgctg cttacatcgg taacggcgac
      781 cgtgctgaaa cctacactgg tggtctgaaa tacgatgcta acaacatcta cctggctgct
      841 cagtacaccc agacctacaa cgcaactcgc gtaggttccc tgggttgggc gaacaaagca
      901 cagaacttcg aagctgttgc tcagtaccag ttcgacttcg gtctgcgccc gtctgtagca
      961 tacctgcagt ctaaaggtaa aaacctgggc accatcggta ctcgtaacta cgacgacgaa
     1021 gatatcctga aatatgttga tgttggtgcg acctactact tcaacaaaaa catgtccacc
     1081 tatgttgact acaaaatcaa cctgctggac gacaaccagt tcactcgtga cgctggcatc
     1141 aacactgata acatcgtagc tctgggtctg gtttaccagt tctaatctcg atggatatcg
     1201 aacaaagggc ctgcgggccc ttttttcatt gttttcagcg tacaaactca gttttttggt
     1261 gtactcttgc gaccgttcgc atgaggataa tcacgtatgg aaataagctt tacccgcgtg
     1321 gcactgctgg ctgccgcgct cttctttgtt ggttgcgatc aaaaaccaca acccgccaaa
     1381 acccacgcta ctgaagttac cgttcttgaa ggcaaaacca tgggtacctt ctggcgtgcc
     1441 agcatcccgg gcattgatgc caaacgcagt gccgaactta aagaaaagat tcagacccag
     1501 ctggacgccg acgatcagct gctttcgacc tataaaaaag attccgcgct gatgcgcttt
     1561 aacgactcgc aaagtttgtc gccgtggccg gtaagtgaag cgatggccga tatcgtcacc
     1621 acctcgctgc gcattggcgc aaggaccgat ggtgcgatgg atataaccgt cgggccgctg
     1681 gtgaatctgt ggggctttgg cccggaacaa cagccggttc aaattccgag ccaggaacag
     1741 atcgatgcga tgaaagccaa aaccggctta cagcacctga cggtcattaa tcagtcgcat
     1801 cagcaatatc tgcaaaaaga cctgccggat ttatatgtcg atctctccac cgtcggtgaa
     1861 ggttatgcgg cggatcacct ggcacgcttg atggagcagg aaggtatttc acgttatctg
     1921 gtgtcggtgg gcggcgcgct gaacagccgt ggtatgaacg gtgaaggcca gccgtggcgg
     1981 gtagcgattc aaaaaccaac cgataaagaa aacgaggttc aggccgtggt ggatatcaac
     2041 ggtcatggca ttagcacctc tggcagctac cgtaactatt acgaactgga cggcaaacgt
     2101 ctttctcatg ttatcgatcc gcaaaccggg cgtcctatcg aacacaatct ggtatccgtg
     2161 acggtgattg ccccgacggc gctggaagcc gatgcctggg atactggctt aatggtactc
     2221 gggccggaga aagccaaaga ggttgttcgc cgggaagggc tggcggtcta tatgatcacc
     2281 aaagaaggtg atagctttaa aacctggatg tcgccgcagt ttaaaagctt ccttatcagc
     2341 gaaaaaaatt aaagcgcaag attgttggtt tttgcgtgat ggtgaccggg cagcctaaag
     2401 gctatcctta accagggagc tgattatgaa aaaagccaca tgcttaactg acgatcaacg
     2461 ctggcaatct gtcttagccc gcgacccgaa tgccgacggc gaattcgttt tcgccgtgcg
     2521 taccacaggc atcttttgcc gtccgtcttg ccgcgccaga catgctttgc gggaaaacgt
     2581 ctccttctac gcaaatgcca gcgaggcact cgccgctggc tttcgcccct gcaaacgttg
     2641 tcagccagac aaagccaatc cccggcaaca tcggttggat aaaatcaccc acgcctgtcg
     2701 actgctggaa caggaaacgc ctgtaacgct ggaagcctta gccgaccatg tggcgatgag
     2761 tccgttccat ctgcatcggt tgtttaaagc tactaccgga atgacgccta aagcctggca
     2821 acaggcctgg cgcgctcgcc gtttgcgcga atcgctggcg aaaggggaga gcgtgacgac
     2881 gtctattctt aacgccggat tccccgacag cagcagttac tatcgcaaag ctgacgaaac
     2941 gctgggcatg acggctaaac aattccgtca cggtggcgaa aatctggcgg tgcgttacgc
     3001 gctggctgat tgtgagctgg gtcgttgcct ggtggcagaa agcgagcggg ggatttgcgc
     3061 gatattgctg ggcgatgatg acgcgacact aatcagcgag ttgcagcaga tgtttcccgc
     3121 tgccgacaac gcgcctgccg atctgatgtt tcagcaacat gtgcgtgaag tgatcgccag
     3181 cctcaatcaa cgcgatacgc cgctgacgtt accgctggac attcgcggca ctgcttttca
     3241 gcaacaagtc tggcaggcac tgcgcacgat accttgcggt gaaaccgtca gttatcagca
     3301 actggctaac gccatcggca aaccgaaagc ggtacgggcc gttgccagcg cctgtgccgc
     3361 caacaagctg gctatcgtaa taccctgtca tcgagtggtc cgtggtgatg gcacactttc
     3421 cggttaccgc tggggcgtgt cgcgtaaagc gcaactgctg cgccgcgaag ctgaaaatga
     3481 ggagaggtaa tgttggatct gtttgccgat gctgaaccgt ggcaagagcc actggcggct
     3541 ggagcggtaa ttttgcaccg ttttgctttt aacgctgcgg agcaactaat ccgcgatatt
     3601 aatgacgttg ccagccagtc gccgtttcgc cagatggtca cccccggggg atataccatg
     3661 tcggtggcga tgaccaactg tgggcatctg ggctggacga gccatcggca aggttatctc
     3721 tattcgccca ttgatccgca aacaaataaa ccgtggcccg ccatgccaca gagttttcat
     3781 aatttatgtc aacgtgcggc tacggcggcg ggctatccag atttccagcc agatgcttgt
     3841 cttatcaacc gctacgctcc tggcgcgaaa ctgtcgctgc atcaggataa agacgaaccg
     3901 gatctgcgcg cgccaattgt ttctgtttct ctgggcttac ccgcgatttt tcaatttggc
     3961 ggcctgaaac gaaatgatcc gctcaaacgt ttgttgttgg aacatggcga tgtggtggta
     4021 tggggcggtg aatcgcggct gttttatcac ggtattcaac cgttgaaagc ggggt
//
LOCUS       DAPHBJ010000272         3894 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.274, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000272 DAPHBJ010000000
VERSION     DAPHBJ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.274"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            70..1728
                     /gene="dpiB"
                     /locus_tag="RHZ76_004299"
     CDS             70..1728
                     /gene="dpiB"
                     /locus_tag="RHZ76_004299"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HDV2814810.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKTIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1697..2377
                     /gene="dpiA"
                     /locus_tag="RHZ76_004300"
                     /gene_synonym="citB"
     CDS             1697..2377
                     /gene="dpiA"
                     /locus_tag="RHZ76_004300"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HDV2814811.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2418..3803)
                     /gene="dcuC"
                     /locus_tag="RHZ76_004301"
     CDS             complement(2418..3803)
                     /gene="dcuC"
                     /locus_tag="RHZ76_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDV2814812.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLTHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1057 a          934 c         1008 g          895 t
ORIGIN      
        1 ctatgtattt tgattcaaca ttttaattac atccgtcaaa gaggctcggg acaacccgca
       61 aggaaaacaa tgttgcagct taacgagaat aaacagtttg catttttcca aagactggca
      121 tttccgctgc gtatcttttt gctgattctg gtgttctcaa tatttgtcat tgcagccctg
      181 gcgcaatatt ttacggccag ttttgaggac tatttaacgc ttcatgtacg cgacatggca
      241 atgaatcagg cgaaaattat tgcctccaat gacagtgtca tctctgcggt gaaaacgcgt
      301 gactacaaac ggctggcgac catcgctaac aaattacaaa gagataccga ttttgattat
      361 gtggtgattg gggaccggca ctcgatccgc ctttaccatc ctaatccgga gaaaattggt
      421 tatcctatgc agttcaccaa acagggcgcg ctggagaaag gggagagcta cttcattacc
      481 gggaaagggt caatggggat ggcgatgcgc gccaaaacgc caatctttga tgacgatgga
      541 aaagtcatcg gcgtggtgtc gattggctac ctggtgagta aaatcgatag ctggcgggct
      601 gagtttttat taccgatggc aggtgtgttt gtcgtgctgt tagggattct gatgttgctg
      661 tcgtggttcc tggccgcgca tatccgtcgg cagatgatgg gcatggagcc aaagcaaatc
      721 gcacgcgtgg tccgtcagca agaggcgctg tttagttcgg tttatgaagg gctgattgcg
      781 gtggatccgc acggttacat taccgccatc aatcgtaacg caagaaagat gctggggctg
      841 agttcccccg gacggcaatg gttgggtaaa accattgctg aagtggtcag gcccgccgat
      901 ttctttaccg aacagattga tgaaaaacgt caggatgtgg tggctaactt taacggtctg
      961 agcgttattg ccaatcggga agctattcgt tcaggtgatg atttgctggg ggccattatc
     1021 agctttcgca gtaaagacga aatttccacc ctcaatgcgc aactgacgca aataaaacaa
     1081 tacgttgaga gccttcgtac attgcgacac gagcatctca attggatgtc gacgctcaat
     1141 ggtctgttgc agatgaaaga gtatgatcgc gtgctggcga tggtgcaggg ggagtctcag
     1201 gcccagcaac agcttattga cagcctgcgc gaggcgtttg ccgatcgcca ggtggcgggg
     1261 ctgctttttg gtaaagtgca gcgcgcccgg gaactggggc taaaaatgat cattgtcccc
     1321 ggtagccagc tttcgcaact gccgccagga ctggatagca ccgagtttgc agccattgtg
     1381 ggcaatttac ttgataacgc cttcgaagcc agcctgcgta gcgatgaagg aaacaagatc
     1441 gttgaattat tcctcagcga tgaaggcgat gatgtggtga ttgaagtcgc cgatcagggc
     1501 tgcggcgttc cagagtctct acgagacaaa atatttgagc agggggtcag tacgcgtgct
     1561 gacgagcccg gtgaacatgg cattgggttg tacttgattg ccagctacgt aacgcgctgc
     1621 ggtggtgtta tcactctcga agataatgat ccctgcggta ccttattttc aatctatatt
     1681 ccgaaagtga aacctaatga cagctccatt aaccctattg atcgttgagg acgaaacgcc
     1741 gctggcagag atgcatgcgg aatatattcg tcacattccc ggattcagtc agatattact
     1801 ggcgggaaac ctggcgcagg cccgaatgat gatcgagcgt tttaagccgg ggctaatctt
     1861 gctcgataac tatcttcctg acggcagagg gattaattta ttgcatgaac tggtgcaggc
     1921 gcattatccc ggcgacgtgg tgtttaccac tgcagccagc gatatggaaa cggtgtctga
     1981 agccgtacgt tgtggtgtat ttgattatct cattaagccc attgcctatg aacggctggg
     2041 gcaaacgcta acccgtttcc gccagcgtaa acatatgctg gaaagtattg atagcgccag
     2101 ccagaagcaa attgatgaga tgtttaatgc ctatgctcgc ggtgaaccta aagacgaact
     2161 gccgaccggc attgatccct tgacgctaaa cgcggtgcga aaactgttta aagagcctgg
     2221 tgtgcaacat acggcagaaa cggtggcgca ggcactgacc atcagccgca ccactgccag
     2281 acgttatctt gaatattgcg ccagccgcca tctgattatt gctgaaattg ttcacggcaa
     2341 agttggcaga ccacaacgca tataccacag tgggtgacat gataaagccg gaggaaactt
     2401 gcctccggca ttactattta cttgcctgtg accgctgctg ccgttcctgg caccatcagc
     2461 tctgtagcaa cgataacaat caccaaacca acaagcaccg gtaccgaggt gcgttttacg
     2521 acttcaaacg gcgagatctt cgccatcccg gcaaccgcaa cgactacgcc agaaacgggc
     2581 gaaagggtac ggccaaggtt tgacgcctgc agcatcggga tagtcaaata cgccgggtta
     2641 atgccggaag agtgcgtcag tttcgggatc atctcaacaa acgcataaaa cggcgcattg
     2701 cctgaaccgg tcgtgactgc cgccagcatt gtcagaatca ccaataccag catcaggatg
     2761 atactcgccg aaccaaacga ggtagcgata gaaatcagac tttgaataaa gccgatggtg
     2821 ctaagcccct gagcgaatac cccagcggca accagcagca tcaccacgtt agcaaacgca
     2881 tctgccatcc cgcgataagc cacttccaga ccagagaaaa ctttctgggt attaaagctg
     2941 cggaggaact ccagaatgga ggcaatcagc atacaaatca ccagaatagt gatgatgtgt
     3001 aattgcggac cccatttacc gtcaaaaatc agtacaccga tgatcggcgt gaacggcaaa
     3061 atggcataaa acgcaggagc agtggtggtg atttcactga catctaacat ttcatgagag
     3121 atgtgctctt ttttatccag ataacgttgc cagaagaagt gggcgatcgc catgccgata
     3181 attgcagcaa ttgagatagg cagcgtcgtt ttgaaggcga agtcaatcag cgacatttcg
     3241 gaagcttgcg ccgccagcac cacatcccct gaagtcggtg cgagaataat cgccgccggg
     3301 gaggcacaaa tggcagcagc tgcgccacga ctgataccaa cgtttaccat caccggaaat
     3361 agggttgcca tcagcaaaac acccagaccg gttgcggaag agacggccag agacatcaga
     3421 caggcgacaa aataggcggc aatcatcagc aggtaagggg agttaatata ctgcaatggt
     3481 tttgacgcca gcttgaccac catatcattc gcgccgatat gggtcatgta agcggcaaat
     3541 ccacacagca tcataatcat catgccgagg tcgccgccgc ggctcattag taatatttta
     3601 acgtattcaa cgatatccgt ggcgctgtag cctgttgaag cctggctgga cggtaacact
     3661 ttgtgcccca taatggcact gataatcagc aataacaggc caccgacaaa taacacacca
     3721 gtggcggaat accctttaat gatgtagcga gctacaccca caataaccac aaccccaata
     3781 aggagctcaa tgaatgtcag cataattttt cctgtctcca ggccccaaag taaataataa
     3841 aaaattccta aagcttaagg aaaaaatatg cccaataaat tggcgatgaa tgct
//
LOCUS       DAPHBJ010000273         3865 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.275, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000273 DAPHBJ010000000
VERSION     DAPHBJ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.275"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            200..1345
                     /locus_tag="RHZ76_004302"
     CDS             200..1345
                     /locus_tag="RHZ76_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2814813.1"
                     /translation="MTEQTSPAQLDLDSAAQPPRVYIRGDWVLAHYCVLEPRVAQLRA
                     NMPSGGVFDLNQLGALDTAGATLLALLLGEDRVANLRELAPKLPEERRILLETVSHVL
                     PDLTPELSEKPTRFWLEMLANTGRSVDNLWQDIKSLLGFIGLALETLLGTLFRPSRWR
                     ITSLIANIQQIGLNAVPIIMLLTFLVGAVIAFLGATVLTTFGAGIFTVDLVVFSFLRE
                     FAVLLTAILMAGRTASAFTAEIGLMKANEEIDAIQTLGLNPVELLVLPRVLALLISLP
                     MLTFIGMVCGIFGGMVVCALTLDISPTMFLSIMQNSNGLQHFLVGISKAPIFAFLIAI
                     IGCLEGFKVTGSAESVGVHTTTSVVHSIFVVILLDAVAALFFMEMGW"
     gene            1351..2142
                     /locus_tag="RHZ76_004303"
     CDS             1351..2142
                     /locus_tag="RHZ76_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2814814.1"
                     /translation="MTQDAIIQIRDLVNCFGVQCVHQGLNLDVKRGEVLGVVGGSGTG
                     KSVLLRSIVGLRRPTDGKIHVFGKDLMALSGQSRAMVERRFGVLFQRGALFSSLTVTE
                     NVALPLIENAGLPRSEAERLAQVKLALAGLPPGTGSKYPASLSGGMVKRVALARALAL
                     DPDILFLDEPTAGLDPIGAAAFDSLIRTLRDALNLTVFLVTHDLDTLYTLCDRVAVLS
                     QKKVLVVDTLDKVAATDDGWIQAYFHGPRGRAAYQAAAINSGERL"
     gene            2144..3082
                     /locus_tag="RHZ76_004304"
     CDS             2144..3082
                     /locus_tag="RHZ76_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005158681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HDV2814815.1"
                     /translation="METRAHHVVIGLFTLIVVSAALLFCLWLTNSGSDRQFRLYDIVF
                     NEPVSGLSQGSTVQYSGIRVGEVTQLRLDRDNPNKVWARIRVSASTPIREDTQARLTV
                     AGITGTSNIQFSSGSQTSPLLEGKDDEVPIIIATPSPMSQLLANGENFMTNANEVLVR
                     LNQLLAPDNQQRLVTTLDNLALVTQTVADQRGDLRTLLQQLAQASKQANETLTQTNRL
                     LRNANGLMDGQGKQLVSDAAKTMASLQNTSTILNKLVSENKTSLNNGMQGMNELGPAV
                     DELRRTLATLRAAVSRLEENPAALLRGRERTQEFTP"
     gene            3082..3723
                     /locus_tag="RHZ76_004305"
     CDS             3082..3723
                     /locus_tag="RHZ76_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HDV2814816.1"
                     /translation="MLSFNVMRYAGRRVWLLMISGGAILLSACTILPSAPVSQVYLLP
                     VPPAVSVPHAQTVNWSLRVSQPATNQFLNSSRIAVQPQGQEIAVYKNSRWSDSAPILV
                     RNRLIQAFRSDGRIRAVSSDDDSLQADVELSGDLSAFQGVYHVGSSEVLIRFDARLVR
                     ISDRRIIATRQFDIRQPIKGNEMNEVVQAFGLASDQLATQVLNWTMQQSPAQG"
BASE COUNT          859 a          884 c         1055 g         1067 t
ORIGIN      
        1 taattctgct ggcggtagtc gtttggttta ccctgccaga gaggaaaata cggcaattgg
       61 gcaaacagcg tgccacacct cactgattag ggtgtggtta acacttcatt aattaaacag
      121 tcattactct tacttaactg tgtgatatac ctatttaggg tttgaaacat tagagtttga
      181 aataggtaag gtctgagcaa tgactgagca aacgtccccc gcacaacttg atctcgatag
      241 cgcggctcag ccccccagag tttatatccg gggggactgg gtgttggcgc attactgcgt
      301 tttggagccg cgcgttgcgc aattgcgcgc taatatgccc agtggggggg tatttgatct
      361 caaccaactg ggggcgttag ataccgccgg tgcaacgttg ttagctctgc ttctgggtga
      421 ggaccgtgta gcaaatttac gcgaacttgc gcctaaattg ccagaagagc ggaggatatt
      481 acttgaaacg gtaagccatg tattgcctga cttaacccct gagttgtctg aaaaacccac
      541 acggttttgg cttgagatgc tggctaatac cggccgttcg gtagataact tatggcaaga
      601 tattaagtca ctattgggct ttattggtct ggcgctggaa accttgctgg gaacactttt
      661 tcgcccctcc cgctggcgca ttacctcatt aattgccaat attcaacaga ttggcctcaa
      721 tgctgtgccg atcattatgt tgctcacctt tttggtgggg gcagttatcg cctttctcgg
      781 tgcaaccgta ctcactactt tcggcgcggg catttttacc gtcgatttgg tggtattttc
      841 ttttctacgt gaatttgcgg tactgcttac cgcgatcctg atggcagggc gaacagccag
      901 tgcttttacc gctgaaattg gcttgatgaa agccaatgaa gagattgatg ctatccaaac
      961 actgggcttg aatccggtcg aactgttggt gctaccgcgc gtcttggcac tattgatctc
     1021 tctgccgatg ctaaccttta ttggcatggt ttgcggtatt ttcggtggca tggtggtgtg
     1081 tgccttaaca ctggatatct ctcccactat gtttttgtcg attatgcaaa acagtaatgg
     1141 gttgcagcat tttctggtgg gaataagtaa agcccctatt tttgctttcc tgattgctat
     1201 tattggctgt ctggaggggt tcaaagtaac cggtagtgct gaatcagtcg gggttcatac
     1261 caccaccagt gtggtgcatt cgatctttgt ggtgattttg cttgatgccg tggcagcgct
     1321 atttttcatg gaaatggggt ggtgagccaa atgacgcaag atgcaatcat tcaaattcgc
     1381 gatctggtta actgctttgg cgtacagtgc gtgcatcaag ggttgaatct tgatgttaag
     1441 cgcggcgaag ttcttggcgt ggttggtggc tcgggtaccg gtaaatctgt tctgttacgc
     1501 agtattgttg ggttacgtcg tccgactgac gggaaaattc atgtttttgg caaggattta
     1561 atggcactgt caggtcaatc ccgcgccatg gtcgagcgcc gattcggcgt attatttcaa
     1621 cgaggcgcac tgttcagttc tctaaccgtg accgagaacg tggcattacc attaatagaa
     1681 aacgccggat taccccggtc tgaggctgag cgattggctc aggttaaact ggcactggct
     1741 gggctaccgc cgggcactgg cagtaagtat ccagcatctc tgtcgggtgg tatggtcaag
     1801 cgtgttgcgc tagcccgtgc cttagcactg gacccggata ttctgtttct tgatgaaccg
     1861 actgcgggtc ttgatcccat tggtgcggcg gcgtttgaca gtttgatccg caccctgcgg
     1921 gatgccctca atctaacggt attcttggtg acccacgatc tggatacgct gtatacactg
     1981 tgtgatcgtg tggcggtgct ttcgcagaaa aaagtgctgg tggttgatac tctggataaa
     2041 gttgcagcga ctgatgatgg ctggattcag gcgtattttc atggcccacg tggccgggct
     2101 gcttatcagg cagcggcaat taacagtgga gagaggttgt aatatggaaa cccgtgctca
     2161 tcatgtcgtt attggtttgt tcactcttat tgtggtgagt gccgcgctgc tgttttgcct
     2221 atggctgaca aattccggtt ctgatcgcca gttcagattg tacgatatcg ttttcaatga
     2281 gcctgtgagt ggcctgtcac agggtagcac cgttcagtac agcgggattc gagtcggtga
     2341 ggtgacgcaa ttacgcctgg accgagacaa tccgaataag gtttgggcac gtattcgcgt
     2401 ttctgcatct actccgatac gcgaagatac ccaagctcgt ctcaccgtcg cgggtattac
     2461 cggcacctca aatattcaat tcagcagtgg cagccagacc agcccgttgc tggagggcaa
     2521 agatgacgag gttcctatca ttattgctac cccttcgccg atgagccagt tactggctaa
     2581 cggtgagaat ttcatgacca atgcgaatga agtgttggtg cgtttgaacc agttgctggc
     2641 accggataac cagcagcgcc tagtaacgac actggataat ctcgcgctgg tcacccaaac
     2701 tgtggctgat caacgtggtg atctccgtac cctgttgcag cagttggcgc aggccagtaa
     2761 acaggctaat gagacactga ctcaaaccaa tcgcctgctg cgtaatgcta acggcttaat
     2821 ggatgggcag gggaaacaac tggtcagtga tgccgccaaa accatggcat cgttgcaaaa
     2881 taccagtacc atcctcaata aattggtgag tgaaaataaa acctcactca ataacgggat
     2941 gcaagggatg aatgagctgg ggccggcagt agatgagctg cgcagaacat tagcgacatt
     3001 acgtgccgcc gtttctcgtt tggaagaaaa cccagcggcc ttgttgcgcg gccgtgaaag
     3061 aacacaggag tttacgcctt aatgctctct tttaatgtga tgcgctatgc aggccgccgc
     3121 gtgtggctat tgatgatatc cgggggggcg atactgctct ctgcctgcac cattttacct
     3181 tcggcccctg tatcacaagt ttatcttctg ccggtgccac ctgcagtcag tgtgccacac
     3241 gcgcagacag taaactggtc attacgggtt tcacaaccgg cgacgaacca gtttcttaat
     3301 agctcacgca tagcggtgca gcctcagggc caggagattg cggtttataa aaactcccgt
     3361 tggagtgatt cggcgccgat tttggtgcgc aatcgtctta ttcaggcgtt ccgtagcgat
     3421 gggcgaatcc gtgcagtcag tagcgacgat gatagcttgc aggctgatgt ggagttgagt
     3481 ggtgatctgt ccgcgttcca aggggtatac catgttggta gcagcgaagt gctgatccgt
     3541 tttgatgctc ggctggtaag aatttcagat cgtaggatta ttgcgactcg ccagtttgac
     3601 attcgccaac cgataaaagg taacgagatg aacgaggtgg ttcaggcctt tggcctggcc
     3661 agtgaccagt tagccactca agtgctcaac tggacgatgc agcaatcccc tgcgcagggt
     3721 tgatatctaa accagcaccg gttggtttta tctgaggcga gttggcagta gctaactcgc
     3781 cttttttatc gtctagttta atgattcaag gcgaataatt gcgtctaagg ggaaaatatt
     3841 ctctttattg ttttgtgatt agtat
//
LOCUS       DAPHBJ010000274         3857 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.276, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000274 DAPHBJ010000000
VERSION     DAPHBJ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.276"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            75..791
                     /gene="nanC"
                     /locus_tag="RHZ76_004306"
     CDS             75..791
                     /gene="nanC"
                     /locus_tag="RHZ76_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HDV2814817.1"
                     /translation="MKKAKILSSVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            811..1917
                     /gene="nanM"
                     /locus_tag="RHZ76_004307"
     CDS             811..1917
                     /gene="nanM"
                     /locus_tag="RHZ76_004307"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HDV2814818.1"
                     /translation="MNKTITALTIIMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKRWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAYYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGDNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSADIHLWYNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVMDSVLISVKDNKVTVQN"
     gene            1982..2962
                     /locus_tag="RHZ76_004308"
     CDS             1982..2962
                     /locus_tag="RHZ76_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HDV2814819.1"
                     /translation="MNAIISPDYYYILTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPHNKFLGVCWMQGEFDLMTSDYAPHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEVIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDKFVEAILQFWRER"
     gene            complement(2970..3620)
                     /locus_tag="RHZ76_004309"
     CDS             complement(2970..3620)
                     /locus_tag="RHZ76_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDV2814820.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIEQGFHIHLDKAAAAPIADVLFPD
                     ELPSQETYLEGAAIKVTVNRYERDKKARDKAVEHHGCQCNVCGVDLVKIYGDIAEGFI
                     HVHHLVPLSAIKEDYQLDPVNDLLPVYPNCHAMLHRRKPPFTPEQLKALMDANKSN"
BASE COUNT         1109 a          846 c          903 g          999 t
ORIGIN      
        1 attacacaac ataaatgcta attgtttatg cgggctttgt attgctttct gtatcctaca
       61 aatgagtgaa atttatgaaa aaggctaaaa tactttctag cgtattatta ctgtgctttt
      121 cgtccccatt aatttctcag gctgcgacac tggacgtacg tggtggatat cgtagtggaa
      181 gccacgccta tgagactcga ctcaaagtca gtgagggatg gcaaaatgga tggtgggcaa
      241 gcatggaaag taatacctgg aataccattc atgataataa aaaggaaaat gccgcactca
      301 atgatgttca ggttgaagtt aattacgcga ttaaacttga tgatcaatgg acggtgcgcc
      361 cgggaatgtt aacgcatttt agcagcaatg gcacacgcta cggaccctac gtaaaactgt
      421 cctgggacgc gacaaaagat cttaattttg gcattcgcta tcgttacgac tggaaagctt
      481 accgacaaca agacttatct ggtgatatgt ctcgtgataa cgttcatcgt tgggatggat
      541 atgtcactta ccatattaat agtgatttca ccttcgcatg gcaaacgacg ctatacagta
      601 aacagaacga ttatcgctat gcaaaccata agaaatgggc gacggaaaat gcatttgttc
      661 tacaatacca tatgacgccc gatattacgc catacataga atatgactac cttgaccgtc
      721 agggtgttta caacggcaga gataatttat cggaaaacag ttatcgcatt ggtgtgtcat
      781 ttaaactgta gtagacagga gacagtcaca atgaataaaa caataacggc gcttactatc
      841 ataatggctt catttgccgc aaacgcgtct gtattaccgg aaactcctgt gccatttaaa
      901 agtggtaccg gagcaattga taacgacact gtctacattg gtttaggtag cgcaggtacg
      961 gcatggtaca agctggatac acaggccaaa gataaaagat ggacagcgtt agctgcattc
     1021 cctggcggac cgagagatca agcaacctct gcatttattg atggcaatct gtatgtgttt
     1081 ggcggcattg gcaaaaacag cgagggcttg actcaggtat ttaatgacgt acacaaatac
     1141 aaccccaaaa ccaatagctg ggttaaattg atgtcgcacg cgccgatggg catggcgggc
     1201 catgtgactt ttgtacacaa cggcaaggct tatgttactg gcggtgttaa ccagaatatc
     1261 ttcaatggct attttgaaga tctcaacgaa gctggaaaag attcaaccgc tatagataaa
     1321 atcaacgcct actattttga caaaaaagca gaagattatt tcttcaataa gtttctgttg
     1381 tcttttgatc cctcaacaca gcaatggagt tacgctggcg aatctccctg gtacggaacg
     1441 gctggtgcgg cggttgtgaa taaaggtgat aaaacctggc ttattaatgg cgaagccaaa
     1501 ccaggattgc gaacggatgc cgtatttgaa cttgacttca ccggtgataa tttaaaatgg
     1561 aataagcttg atcccgtctc atcaccagat ggcgtcgctg gcggttttgc ggggataagc
     1621 aatgattctc ttatatttgc tggaggggcc ggattcaaag gttcacgaga aaattaccag
     1681 aacggtaaga actatgcgca tgaaggcctg aaaaaatcat atagcgctga tattcatctt
     1741 tggtataacg ggaaatggga taaatcgggt gaattatcgc aaggtcgggc ctacggagta
     1801 tcattgccct ggaataatag tttattaatt attggcggtg aaactgcagg cggcaaagca
     1861 gtgatggatt cagttttaat ttctgtgaag gataataaag tcacagtaca aaactaacgc
     1921 ttcagggccc cggtaaaggg gccctgctat caatttgtca tttcaattac gcgaaattta
     1981 tatgaacgca ataatatcgc ctgattatta ctatattctt accgttgctg gtcagtctaa
     2041 tgccatggcg tatggcgaag gactgccatt accggacagg gaagatgcgc ctcatcccag
     2101 aattaaacaa ttagcgagat ttgcgcatac gcatcccgga ggcccgtcat gtcactttaa
     2161 cgacattatt ccactgactc actgcccaca cgatgttcag gatatgcagg gttatcacca
     2221 tcctctggca acgaatcatc aaacacagta cggcaccgtt ggccaggcac tgcatatagc
     2281 acggaaatta ctgcccttta ttcctgataa tgcaggggtt ctcatcgttc cgtgttgccg
     2341 tggcggatcg gcttttaccg cgggcagcga agggacatat tcagaacggc acggagccag
     2401 ccatgatgct tgtcgttggg gaacggatac tccgctatac caggatttag tcagcagaac
     2461 gcgagccgca ctggcaaaaa atccgcataa caaattcctc ggcgtatgct ggatgcaagg
     2521 cgaatttgac ttaatgacca gtgactacgc gccacaccct caacacttta atcatatggt
     2581 tgaagccttt cgtagggatc taaaacaata ccattctcag cttaataata ttactgacgc
     2641 accgtggttt tgcggcgata ccacctggta ctggaaagaa aatttccctc atgcgtatga
     2701 agttatttat ggcaattatc aaaataatgt tttagccaat attattttcg tcgacttcca
     2761 gcaacaaggt gaaagaggac tgacgaacgc gcctgatgaa gacccggacg atttaagcac
     2821 gggatattac ggttcagcgt accggtcacc ggagaactgg acgacggcac tgcgaagcag
     2881 tcatttcagc acggcagccc gtcgggggat tatttctgac aagtttgtag aagcaatttt
     2941 gcagttttgg cgcgaaaggt gagcgttcat taatttgatt tattcgcatc catcagcgct
     3001 tttaactgct ccggcgtaaa cggtggtttg cgtcggtgca acatcgcatg gcaattaggg
     3061 tacacaggca gcaaatcatt aactggatcc agctggtaat cctctttaat ggctgacagc
     3121 ggcaccaggt gatgcacatg gataaagcct tcagcgatat caccatagat cttcaccaga
     3181 tcaacaccgc atacgttgca ctggcatcca tgatgttcga ccgctttatc tcgggccttt
     3241 ttatcgcgtt catagcggtt tactgtcacc tttatcgccg caccttcaag gtatgtctcc
     3301 tgcgacggca actcatccgg gaacagaacg tcggctatgg gagccgcagc ggctttatcc
     3361 agatgaatat gaaatccctg ctctatgagt agcttgacac atttagattt tattccgccg
     3421 gtgaaatccg caggggtaaa ttgcgtcccc gtcatcaatg tcgctgcgat gccagcaatc
     3481 gccttcgagg ggtaacgtcg gccatgatat tcaatctcat acaaccgcgc aggctggaac
     3541 tgatgagttt ctcccgcatc ccataaatgg atagcttcga tgatataccg ccgcggaaga
     3601 tgatcaggga gtttattcat ggagaatgtc cttatacgtt ctcagaacag gcagcaaaaa
     3661 aacaaatgag atcaaggtaa tatatcttca cttgcaggct tgaacagcag aaaatttgcg
     3721 agcatgatcg caattatttc actgccaggg atatcaatag cttccagtaa atcgctacag
     3781 caggcaattg ccaatataaa aatctggcac aagggtgaac agcgcgcgcc gcataagcca
     3841 ttgttattgc tatacgt
//
LOCUS       DAPHBJ010000275         3839 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.277, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000275 DAPHBJ010000000
VERSION     DAPHBJ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.277"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(398..1237)
                     /locus_tag="RHZ76_004310"
     CDS             complement(398..1237)
                     /locus_tag="RHZ76_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814821.1"
                     /translation="MLKLELIILPLLAFLGPSNITNASTKTPIPSSQLSDAKTGVNYE
                     FITIAANYVNGMWLVDGRQRPVIKTSMTKRNYLQIENDSASTPLNLVIPKIEFSIIAK
                     NGVFISKFISLDEDASGKRILWLEPGSSMTISFVNDLSSTPLQALVSVTKQKKEVIAI
                     FGPDQGKKFTLPLPSDSQQSSIDYVPSPEIALRINAPKKLQPAIAIPEDASASLLPSH
                     TYKIRAAEKVRTFKFSSFVWVGKRNNLLGGSDYFTKELKIYPGENFDISTIQKTTMRT
                     END"
     gene            complement(1237..2184)
                     /locus_tag="RHZ76_004311"
     CDS             complement(1237..2184)
                     /locus_tag="RHZ76_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814822.1"
                     /translation="MASEHVQTIDLTNFSGRVFFLADPHGHYSTLCNLIHSISEPDEE
                     LIVFSTGNLFDYGPEPMELMTAINTGIFDGRSVRVFSAAGAGEEMLKKLLPINKDRRT
                     YYPSTFLNERWCARGGRWHKQINRNYLEEEICKLLSTQLATVMKVLFKGNISIGICPS
                     DYTDIRQGFNNTYNALLAFNQANVNIFQSQFLYGMDHAVRPVNVSDVNLVVLGRNPVN
                     SIRKAHGLPLTNLPVLIGNCLHINTGSLYMSEISSPALLAPGIPPTTVPAITLVELIL
                     ASTPTLICHQMILNKNGIYTQNTAPLNLDSNDNNIEATL"
     gene            2558..3592
                     /locus_tag="RHZ76_004312"
     CDS             2558..3592
                     /locus_tag="RHZ76_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stbA family protein"
                     /protein_id="HDV2814823.1"
                     /translation="MTELAKTKLAVAESNSTELETLKVVIDDGSKAAKLVCTNNQGDL
                     VPLLTQNSFVADFRVSHDGLIPFNYLIDGLQRFSHHSESSNALETTDVAHQYDEISRL
                     NVHHALHSSGLMPQDVHLYVTLPLSQFYTALGETNNENIQRKKDNLMKPVERYIDGKR
                     VSFNVVSVTVFPESLPAVTRADEIESIESFESSLVIDLGGTTLDVASITGQLEQISSV
                     RGFDRIGCSIVYDEIRRYLDSAKLNASNAYIHHLVDNRENKASLKVSPEDLDGVFNAV
                     NGAVAQLQEKVIKAVTQVEERPHNVFLVGGGSYLIEPAVRKHFDKSKIIMVDNPQFAL
                     SLAIADTVFA"
BASE COUNT         1085 a          742 c          849 g         1163 t
ORIGIN      
        1 tccatctcgt gaagactgtg agatgttctt aaatgagtta ttaataaatg ttaattggtc
       61 attgttatcg taacaaagac caagtaaacc aactgtgggc accgtaaaaa ttgtaactga
      121 actaggtgat atcaactgtt ttccacgtga cgaataggtg caacgactcc cgccgagatt
      181 cagatcagac aactcaataa attctttggt cccattcttg gcgagtaacc agacatcagt
      241 gcctgaactc tcaatcagaa aagtaacttt tccgtcggtg taggcttcag ttctgttgct
      301 cattataggg tcgaacccta ccgaagggag gactgcaatt ttgcttccaa ccgactgaca
      361 tccagtcaca agtaaggtaa gaataaatgc tgaaactcta atcattttca gtcctcatag
      421 tcgttttctg tatggtactg atatcgaagt tttctcctgg gtaaattttc aactctttgg
      481 tgaaataatc tgaaccaccg aggaggttgt tacgctttcc aacccaaacg aacgacgaaa
      541 atttaaaagt ccttactttt tctgccgctc tgatcttgta ggtatgtgaa ggtaataaag
      601 atgcactagc gtcctccggg attgcgatag cgggttgaag ttttttgggg gcattgatac
      661 gtaatgcgat ttctggagag gggacatagt cgattgacga ttgttgtgaa tctgacggta
      721 ggggcagggt gaatttttta ccctgatctg ggccaaaaat ggcaataacc tcttttttct
      781 gctttgttac ggacactaac gcctgtaacg gggtactgga tagatcattg acgaatgaaa
      841 ttgtcatgct ggaaccaggt tctaaccaga gaattcgttt cccggatgca tcttcatcaa
      901 gggagataaa ttttgatata aacacgccat tttttgcaat gatggaaaat tcaattttgg
      961 gtattacaag atttaaagga gttgaagcag agtcgttttc aatctgaaga tagttacgct
     1021 tagtcatgct tgttttaatt actggtcttt gtcgaccatc aacaagccac attccgttga
     1081 cgtaatttgc agcaatagta ataaattcat agttaacccc agttttggca tcagatagct
     1141 gggacgacgg tatcggggtt ttagtcgatg catttgtaat gttggatgga cctaaaaatg
     1201 ccagcaacgg caaaattatc agttctaatt taagcattat aatgtggcct ctatattatt
     1261 atcatttgaa tccaggttaa gtggtgcagt gttttgtgtg taaataccgt ttttattcag
     1321 gatcatttgg tggcaaatca gggtcggtgt agatgccagg atcaactcga ccagtgttat
     1381 tgccggaact gtagtaggcg gaatacctgg tgccagtagt gctgggctac tgatttctga
     1441 catgtacaga gaacctgtat tgatgtggag gcagtttcct atgagtaccg gtaagttagt
     1501 cagcggtagc ccatgggcct ttctgatgct gttaactgga ttccgaccca atactacgag
     1561 gtttacatcg ctgacattca ctggcctgac tgcgtgatcc atgccgtata gaaactgact
     1621 ctgaaaaata ttcacatttg cctgattaaa agcgaggagg gcgttatatg tattgttgaa
     1681 gccctgccgt atgtctgtgt aatcggatgg gcaaatacct atagaaatat tgcctttaaa
     1741 aagcaccttc atcacagtag ccagctgggt ggagagcagt ttgcagattt cctcttcaag
     1801 atagttgcgg ttaatttgtt tatgccatcg cccgcctctt gcacaccacc gttcattaag
     1861 aaatgtgctt ggataataag tcctgcggtc tttatttatg ggtaagagct ttttcagcat
     1921 ctcttctccg gccccggcag ctgagaacac acgaacagaa cggccgtcaa aaatccctgt
     1981 gttgatcgct gtcattaact ccataggctc aggtccataa tcgaagagat ttcctgtgga
     2041 gaaaacgatc aactcctcat caggttctga tatcgaatgg ataaggttac atagcgtcga
     2101 ataatgacca tgagggtctg ccagaaagaa aacccttcct gaaaaattag tcagatcaat
     2161 tgtctggacg tgctcggatg ccataaatgc ctctttctga aattgcatta atactaccgg
     2221 atgaaagtca tcaatttttt cgataagagg ggtaattttt gtgagtgtcc aaaatggagc
     2281 tagaaatcat ttttcggttt ccgagccgta tcaactgtaa tgcacctcag ttgttttatt
     2341 tttggtataa aaacgctgag caaaatatag tttttgcgtg tgttgctcag gtgtacatga
     2401 atctagggtt gtatctggat taaaactgaa ggattctttt ttaacaagga cttacgttag
     2461 tgttttgtcc cgtttaatgt tgacctaaag caaccattgg tgtataaatt ccataacaaa
     2521 agtggtgggc taaataaacc aatacgaggg tttctgcatg actgaattag caaaaacaaa
     2581 actggctgtg gctgaatcaa actctactga acttgagaca ctcaaagttg ttattgatga
     2641 tggtagtaaa gcggcaaaac tggtttgcac taacaatcaa ggtgaccttg tacctttatt
     2701 gactcaaaat agtttcgttg cagatttccg ggtaagccat gacggactta ttccattcaa
     2761 ttatctaatt gatggtcttc aacgattctc gcaccacagc gaatcaagca atgctttaga
     2821 aactaccgac gttgcgcatc agtacgatga aatcagtcgt cttaatgttc accatgctct
     2881 acattcctca ggtctaatgc cacaggatgt tcatctctac gtaactttgc cactcagtca
     2941 gttttatacc gctctgggtg aaaccaataa tgaaaacatc caacgaaaaa aagacaacct
     3001 gatgaagccg gtggagcgtt atattgatgg taaacgtgtt tcatttaatg ttgtttcagt
     3061 taccgttttc ccggaatccc ttccagcggt aacgcgtgct gatgaaatcg aatcaattga
     3121 atcttttgaa tcaagcctgg ttatcgattt aggtgggaca acacttgatg ttgcaagcat
     3181 tacaggtcag ctggaacaga tctccagtgt caggggtttt gaccgtattg gttgctcaat
     3241 cgtatatgat gaaatccgtc gttatttgga ttctgcaaag ctgaatgcaa gtaacgccta
     3301 tattcatcac ctagtcgata atcgtgaaaa caaagcctcg ctcaaggtgt ccccggaaga
     3361 tcttgacggc gtgttcaatg ctgttaatgg ggctgtggcg caacttcaag aaaaagtgat
     3421 taaagcagtc actcaagtgg aagaacggcc gcataatgtt ttccttgttg gtggaggttc
     3481 ttacctgatt gaaccggcag ttcgtaaaca ttttgacaaa tcgaaaatca tcatggttga
     3541 taatccgcaa ttcgcacttt cactggctat cgcagatact gtttttgcat gatgatggtt
     3601 ttgcatgatg atggttttgc aaaggggaga gttttatgac taaaaaatct gagaaggaaa
     3661 acgatcgtat tcagattagt gcgttttggt tatcggaacg acaatctccg tatgcatata
     3721 attttttgaa aaaaaatgcc ctaacacatc gtggcgagca gatttctcta ataagatctg
     3781 caatcactac ggggttagtg ctaaataatc tcttccccga gttatctagt tttattaat
//
LOCUS       DAPHBJ010000276         3648 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.278, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000276 DAPHBJ010000000
VERSION     DAPHBJ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.278"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1097
                     /locus_tag="RHZ76_004313"
     CDS             <1..1097
                     /locus_tag="RHZ76_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008175578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDV2814824.1"
                     /translation="VERHIRGKWACDSCETLIQAPVPPQVIDKGIPTAGLLAQVLVAK
                     YADHLPLYRQERMFGRAGLEIPRSTLAEWVGACGVQLQPLVDALRNTLLEHSVLHADE
                     TPVSMLAPGKKKTHKAYVWAYCTTPFADLKAAIYDFAPSRAGEHARTFLGDWRGKLVC
                     DDYAGYKAGFGNGITEIGCMAHARRKFYDLHEANKSELAAKALEYIGGLYEIERETKD
                     LPPDMRREIRQTKAKPLADALHQWMLAHRQKVPDGSGTAKALDYSLKRWEALTRYLDD
                     GAVPIDNNWVENQIRPWALGRSNWLFAGSLRSGQRAAAVMTLIQSAKLNRHDPYAYLK
                     DVLTRLPTQKNNAIDELLPHNWKPAIPNKV"
     gene            complement(1348..1911)
                     /locus_tag="RHZ76_004314"
     CDS             complement(1348..1911)
                     /locus_tag="RHZ76_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HDV2814825.1"
                     /translation="MNTRLFTFAGGETGVWRVVRMDAVAGAPLPGIPRLDVAAGSVSP
                     QPLGTKWLLRGITSNERYVVREEKDRLVAKQPSLGRAEATCAALIPIRKNPSWWGLAQ
                     DERRKIFEEQSRHIHIGLQYLPAVARRLHHCRDLGESEPFDFLTWFEYSPSDEPGFNR
                     LLAELRASVEWKYVDREIDIRLVHEPA"
     gene            complement(1935..2309)
                     /locus_tag="RHZ76_004315"
     CDS             complement(1935..2309)
                     /locus_tag="RHZ76_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDV2814826.1"
                     /translation="MGVNMRAETESRIFSVDEYVRPSNGEPIRSVVLETNDSVVVVWH
                     AHPGQEIASHVHPHGQDTWTVISGEAEYHQGNGIVTHLKAGDIAIAKPGQVHGAMNSG
                     PEPFIFVSVVAPGNAGFALAEK"
     gene            complement(2374..2937)
                     /locus_tag="RHZ76_004316"
     CDS             complement(2374..2937)
                     /locus_tag="RHZ76_004316"
                     /inference="COORDINATES: protein motif:HMM:NF015038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2814827.1"
                     /translation="MAHQIFRSSCSITPLAVPRRCRSASWRQKGVERIGCWACAAQVH
                     QKTQSKGKIGRAEMALTLINKLYGIERDMEEAGDAQRYQTRQSQSQAFLEQLKARLDK
                     TPPQVTSSKCLGQAINCLASNWLKLIRYIERGTPAHRQQPCRAGEPPSHVHMTKASPV
                     SSRYVMPTHDGVLTVSVRQSIVFPKGA"
     gene            3180..3515
                     /locus_tag="RHZ76_004317"
     CDS             3180..3515
                     /locus_tag="RHZ76_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDV2814828.1"
                     /translation="MAQGGITGRLRYNGQRLRLLSIDTPETYKPRCDAELTKGREATA
                     RLRELVANAQRIEVVDSGQADKYDRRLVHLLIDGRDVGQTLMAEGLAVEWRPGPNAWR
                     ERRRHWCGY"
BASE COUNT          813 a         1045 c         1056 g          734 t
ORIGIN      
        1 ccgttgaacg tcacatccgg ggcaaatggg cctgcgacag ctgcgagacg ttgatccagg
       61 caccggtgcc accgcaggtg atcgacaaag gcatccccac cgctggcctg ctggcccagg
      121 tgctggtggc caagtatgcc gaccatctgc cgctgtaccg tcaggaacgc atgttcggcc
      181 gtgccggcct ggaaatcccg cgctccaccc tggccgaatg ggtgggtgcc tgtggtgtgc
      241 agttgcagcc gctggtggat gcgttgagaa atactctgct ggaacacagt gttctgcatg
      301 ccgatgaaac cccggtcagc atgctggccc cgggcaagaa gaaaacccac aaggcctacg
      361 tctgggccta ctgcaccaca ccgtttgccg acctgaaggc agctatttac gacttcgcac
      421 ccagccgtgc aggcgaacac gcccgaacct tcctgggtga ctggcggggc aagctggtct
      481 gtgatgatta cgctggctac aaggccgggt tcggcaacgg cattaccgaa atcggctgta
      541 tggcccacgc ccggcgcaag ttctatgacc tgcacgaggc caacaagagc gaattggcgg
      601 ccaaggcctt ggaatacatc ggtggactct acgagatcga gcgggaaacc aaagatctgc
      661 caccggatat gcggcgggaa atccggcaaa ccaaagccaa accccttgcc gatgcgctgc
      721 atcagtggat gctggcccat cgccagaaag tgccggatgg ctccggcacg gccaaagccc
      781 tggattacag cctcaaacgt tgggaagcgc tgacgcgcta cctggacgat ggcgctgtgc
      841 ccatcgacaa caactgggtg gaaaaccaga tccgaccctg ggcgctgggg cgcagcaatt
      901 ggctgtttgc gggttcactg cgcagtggtc aacgtgcggc tgcggtcatg accctaatac
      961 agtcagcgaa gctgaatcgg catgatccct atgcctacct gaaggatgtg ctgactcgcc
     1021 tgccgacaca gaagaacaac gccatcgacg agctattgcc ccacaactgg aaaccggcca
     1081 tacctaacaa ggtgtaggcc ggacgtgtat ggagagcctt cttcgaaagc tggacaaggt
     1141 gccaactcta gacggcagga tatgccctgg gagatcgatg agagaaagag gaggccataa
     1201 gtagaaaatt tcccaagcga ctggtggctc gctaggcata cgtgtcgtgt tactcctaag
     1261 ttattagaca cactagattt tccgggcttt gctgggctgc gggtgtcgca ggtgtatgcc
     1321 gcacggagat cgcagttaaa cctgatacta ggccggctca tgcactagcc ggatgtcgat
     1381 ttctctgtcg acatacttcc actccaccga ggcccggagt tcggccaaaa gcctgttaaa
     1441 gcccggctca tcagacgggg aatattcgaa ccacgtcaag aagtcgaagg gttcgctctc
     1501 gcccaggtcc cgacaatggt ggagacgacg ggccacagca gggagatatt ggagcccaat
     1561 gtgaatgtga cgggactgct cctcaaaaat cttccggcgt tcgtcttgag ccagtcccca
     1621 ccatgagggg tttttccgga tagggatcag ggcggcacag gtggcctctg ctcgacccaa
     1681 acttggctgc ttcgccacga gacgatcctt ctcttcacgc acgacataac gctcgttact
     1741 cgttatcccg cgaagaagcc atttggtgcc caaaggctgc ggcgaaacag agccagcggc
     1801 tacgtcgagc cgggggattc cagggagagg ggctcccgcc acggcatcca tccgaacaac
     1861 gcgccaaaca cccgtttctc caccagcgaa cgtaaataat cgtgtattca tcagtttcct
     1921 ctcaggcctt tcgcctattt ctcagccaac gcaaaaccgg cattgcccgg cgcaaccact
     1981 gaaacaaata taaaaggttc ggggccagag ttcattgcac catgtacctg cccaggtttt
     2041 gcgatcgcaa tatctccagc tttaagatga gtgactatgc cgttaccttg atgatattcg
     2101 gcttcacctg agataaccgt ccaagtatct tggccgtgag gatgtacatg ggaagctatt
     2161 tcctgcccag ggtgggcgtg ccaaacaacg acgacagagt cattggtttc cagaacaacc
     2221 gaacgaatcg gttcgccgtt agacggacga acatattcat ccaccgaaaa aatccgcgat
     2281 tcagtttctg ctctcatgtt cacacccatt taaaactgat tggacacgta ctctaaacgc
     2341 aactgcgcct ggacagcaca tgaagatatg tacctaagcg cccttcggga aaactatgct
     2401 ctggcgcaca gagaccgtca atactccgtc gtgggtaggc atgacataac gggaggagac
     2461 gggcgacgct ttggtcatat gtacatgaga aggtggctcg cccgctcggc agggttgttg
     2521 tcgatgggca ggtgtccccc tttcgatgta gcggatcagc tttaaccagt tgctcgcaag
     2581 gcaattgatt gcttgcccaa ggcactttga cgacgtgacc tggggagggg ttttgtccag
     2641 cctagctttc agttgctcca ggaaagcctg gctttgcgac tggcgcgtct ggtatcgctg
     2701 ggcatcgccc gcctcttcca tgtcgcgctc gatgccgtag agcttgttga taagagtcag
     2761 tgccatctcc gctcggccga tcttgccctt ggattgagtt ttctgatgaa cttgcgccgc
     2821 gcatgcccag caacctatac gctcgacgcc tttctgcctc caggaggcgc tgcggcacct
     2881 cctgggtacg gctagcggtg taattgaaca ggatgaccgg aagatttggt gggccaccgc
     2941 gatctcctca gtagctatag ccaaacaggt tgggtatgag aagttcaagg gggggcgagc
     3001 aaagcccccg ttccaattga ttctctattc cacacaaatg gcgatataag ccggcgggtg
     3061 atgacgaccc tggaagctct ggcatcgaga gtggatgtct actccatcga cgaggcgttt
     3121 ctggatctga ccggaatcga ccgggtggta agcttcgagc cttttggtcg gcaggcgatt
     3181 tggctcaagg tggaatcacc ggacggttac gatacaacgg tcaacgcttg cgcctgctgt
     3241 cgattgacac cccggaaacc tacaagccca ggtgcgacgc cgagctgacg aagggccgcg
     3301 aggcaaccgc acgcctccgc gaactggttg ccaacgctca gcgcatcgag gttgtcgatt
     3361 ccggccaagc cgacaagtac gaccgccgcc ttgttcacct gctgatcgac gggcgggacg
     3421 tggggcaaac cctcatggcc gaggggttag ccgttgagtg gaggcccggc ccgaatgcgt
     3481 ggagggaacg ccgtcgccac tggtgcggat actgatcgct tttcagcttg agccaggagc
     3541 gccccgccgc agtgatcggc acttgtgtcg atccgaggag gcgcaagagc cacaagcccc
     3601 tgataggacc acgtaacgcg gccatgccac tagagcacgg ccgctttt
//
LOCUS       DAPHBJ010000277         3613 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.279, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000277 DAPHBJ010000000
VERSION     DAPHBJ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.279"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..307)
                     /locus_tag="RHZ76_004318"
     CDS             complement(<1..307)
                     /locus_tag="RHZ76_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814829.1"
                     /translation="MSQNDIIIRTHYKSPHSLHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENILITLHYKKEII
                     "
     gene            831..2759
                     /gene="thrS"
                     /locus_tag="RHZ76_004319"
     CDS             831..2759
                     /gene="thrS"
                     /locus_tag="RHZ76_004319"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDV2814830.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSDYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2763..3305
                     /gene="infC"
                     /locus_tag="RHZ76_004320"
     CDS             2763..3305
                     /gene="infC"
                     /locus_tag="RHZ76_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDV2814831.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3402..3599
                     /gene="rpmI"
                     /locus_tag="RHZ76_004321"
     CDS             3402..3599
                     /gene="rpmI"
                     /locus_tag="RHZ76_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDV2814832.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
BASE COUNT         1005 a          778 c          911 g          919 t
ORIGIN      
        1 aaatgatttc ttttttatag tgtaatgtaa tgagtatatt ttctgcatgg atgggtgaag
       61 tggcaaagtt aatggtaaaa agggcagtgg aataacaatt ttgtgatatt tttcgacagt
      121 ttgcggtaaa ttcctgagta acacagtatg acagcatcga acttaagtca gaggtatcaa
      181 gtagggtgat gagctggcgc gcaaattgat taataggctc tgatgaaggt gttggtatgt
      241 cgctatcgat gtgcaagcta tgaggagact tataatgagt tctgataatg atatcgtttt
      301 gactcataat ctgttcgcct tatatatatc tggaggtttt gaggatgatg attaattatt
      361 atcgccagaa cgaaatgtgc acgaaaaatt gttataagaa ttgtgtcact tttatcattg
      421 tgatatgacc acttcgtcat taatctcatg agcgtttaaa atactaccgc caaatcacgc
      481 aacgaacttt gctggattca gtttatgctg ctgtaaatcc gctcgcagta aacctttcag
      541 acgcaccgtg atgttatcag ttgttcttta agcgttttgc tggtgtactc actacaaacg
      601 aattgcgaat cgaatcaatg tgaaacggaa aggtacaatc tccctttttt tagtgttaac
      661 atcgctcaac cgggtggaag gtgagaggtt aatgtctttc atttgtgtac ctgattgcga
      721 accaatttag catttgttgg ctagatggtt tcgcaatgaa ctgttaataa acaaattttt
      781 ctttgtatgt gatctttcgt gtgggtcacc actgcaaata aggatataaa atgcctgtta
      841 taactcttcc tgatggcagc caacgccatt acgaccacgc tgtaagcccc atggatgttg
      901 cgctggacat tggtccaggt ctggcgaaag cctgtatcgc agggcgcgtt aatggcgaac
      961 tggttgatgc ttgcgatctg attgaaaacg acgcacaact gtccatcatt accgccaaag
     1021 acgaagacgg tctggagatc attcgtcact cctgtgcgca cctgttaggg cacgcgatta
     1081 aacaactttg gccgcatacc aaaatggcaa tcggcccggt tattgacaac ggtttttatt
     1141 acgacgttga tcttgaccgc acgttaaccc aggaagatgt cgaagcactc gagaagcgga
     1201 tgcatgagct tgctgagaaa aactacgacg tcattaagaa gaaagtcagc tggcacgaag
     1261 cgcgtgaaac tttcgccaac cgtggggaga gctacaaagt ctccattctt gacgaaaaca
     1321 tcgcccatga tgacaagcca ggtctgtact tccatgaaga atatgtcgat atgtgccgcg
     1381 gtccgcacgt accgaacatg cgtttctgcc atcatttcaa actaatgaaa acggcagggg
     1441 cttactggcg tggcgacagc aacaacaaaa tgttgcaacg tatttacggt acggcgtggg
     1501 cagacaaaaa agcacttaac gcttacctgc agcgcctgga agaagccgcg aaacgcgacc
     1561 accgtaaaat cggtaaacag ctcgacctgt accatatgca ggaagaagcg ccgggtatgg
     1621 tattctggca caacgacggc tggaccatct tccgtgaact ggaagtgttt gttcgttcta
     1681 aactgaaaga gtaccagtat caggaagtta aaggtccgtt catgatggac cgtgtcctgt
     1741 gggaaaaaac cggtcactgg gacaactaca aagatgcaat gttcaccacg tcttctgaga
     1801 accgtgaata ctgcattaag ccgatgaact gcccgggtca cgtacaaatt tttaaccagg
     1861 ggctgaagtc ttatcgcgat ctgccgctgc gtatggccga gtttggtagc tgccaccgta
     1921 acgagccgtc aggttcgctg catggcctga tgcgcgtgcg tggatttacc caggatgacg
     1981 cgcatatctt ctgtactgaa gaacaaattc gcgatgaagt taacggatgt atccgtttag
     2041 tctatgatat gtacagcact tttggcttcg agaagatcgt cgtcaaactc tccactcgtc
     2101 ctgaaaaacg tattggcagc gacgaaatgt gggatcgtgc tgaggcggac ctggcggttg
     2161 cgctggaaga aaacaacatc ccgtttgaat atcaactggg tgaaggcgct ttctacggtc
     2221 cgaaaattga atttaccctg tatgactgcc tcgatcgtgc atggcagtgc ggtacagtac
     2281 agctggactt ctccttgccg tctcgtctga gcgcctccta tgtgggcgaa gacaacgagc
     2341 gtaaggtacc ggtaatgatt caccgcgcaa ttcttgggtc gatggaacgt ttcatcggta
     2401 tcctgaccga agaattcgct ggtttcttcc cgacctggct tgcgccggtt caggttgtta
     2461 tcatgaatat taccgattca cagtctgatt acgttaacga attgacgcaa aaactatcaa
     2521 atgcgggcat tcgtgttaaa gcagacttga gaaatgagaa gattggcttt aaaatccgcg
     2581 agcacacttt gcgtcgcgtc ccatatatgc tggtctgtgg tgataaagag gtggaatctg
     2641 gcaaagttgc cgttcgcacc cgccgtggta aagacctggg aagcatggac gtaaatgaag
     2701 tgatcgagaa gctgcaacaa gagattcgca gccgcagtct taaacaattg gaggaataag
     2761 gtattaaagg cggaaaacga gttcaaacgg cgcgccctaa ccgtatcaat ggcgaaattc
     2821 gcgcccagga agttcgctta acaggtctgg aaggcgagca gcttggtatt gtgagtctga
     2881 gagaagctct ggagaaagca gaagaagccg gagtagactt agtcgagatc agccctaacg
     2941 ccgagccgcc ggtttgtcgt ataatggatt acggcaaatt cctctatgaa aagagcaagt
     3001 cttctaagga acagaagaaa aagcaaaaag ttatccaggt taaggaaatt aaattccgtc
     3061 ctggtacaga tgaaggcgac tatcaggtaa aactccgcag cctgattcgc tttctcgaag
     3121 agggtgataa agccaaaatc acgctgcgtt tccgcggtcg tgagatggcg caccagcaaa
     3181 tcggtatgga agtgcttaat cgcgtgaaag acgatttgca agaactggca gtggtcgaat
     3241 ccttcccaac gaagatcgaa ggccgccaga tgatcatggt gctcgctcct aagaagaaac
     3301 agtaaggcct tcaagtagca ctgtctgtgg agccttcggg cttcacaggc tttgttcgcc
     3361 tttgtttcgt ttattaacaa tgcgaagtgg aagttattaa aatgccaaaa attaagaccg
     3421 tacgcggtgc tgctaagcgc ttcaaaaaaa ccggtaaagg tggttttaag cacaagcacg
     3481 ctaacctgcg tcacattctg accaaaaaag cgaccaaacg taaacgtcac ctgcgtccga
     3541 aagccatggt ttccaaaggc gatctgggcc tggtaatcgc gtgcctgccg tacgcataag
     3601 ccgttaacgt ttt
//
LOCUS       DAPHBJ010000278         3538 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.281, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000278 DAPHBJ010000000
VERSION     DAPHBJ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.281"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            144..1019
                     /locus_tag="RHZ76_004322"
     CDS             144..1019
                     /locus_tag="RHZ76_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-1"
                     /protein_id="HDV2814833.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQS
                     GGRLGVALINTADNSQILYRADERFAMCSTSKVMAVAAVLKKSESEPNLLNQRVEIKK
                     SDLVNYNPIAEKHVDGTMSLAELSAAALQYSDNVAMNKLISHVGGPASVTAFARQLGD
                     ETFRLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNT
                     TGAASIQAGLPASWVVGDKTGSGDYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESR
                     RDVLASAAKIVTNGL"
     gene            complement(1066..1518)
                     /locus_tag="RHZ76_004323"
     CDS             complement(1066..1518)
                     /locus_tag="RHZ76_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013023839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HDV2814834.1"
                     /translation="MRPARLSTLLVRSLASLYRPCLRGSWLYSRSVFNLLPGTYVRPH
                     RHPHTFELLLPLRGRFVVLNFDDRGTVTHRAILGETCTVLEMAAGTWHAVLSLDTGGI
                     IFEVKHGGYQPVAADDYAHWAPAEGEPGTTELMAWYAQAQVGDSAFAV"
     gene            complement(1409..2593)
                     /locus_tag="RHZ76_004324"
                     /pseudo
     CDS             complement(1409..2593)
                     /locus_tag="RHZ76_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(2590..3495)
                     /locus_tag="RHZ76_004325"
     CDS             complement(2590..3495)
                     /locus_tag="RHZ76_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HDV2814835.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
BASE COUNT          800 a         1054 c         1073 g          611 t
ORIGIN      
        1 agtctaaatt cttcgtgaaa tagtgatttt tgaagctaat aaaaaacaca cgtggaattt
       61 aggttagact ataaatagaa aaaggcgttt tgacagacta ttcatgttgt tgttaattcg
      121 tctcttccag aataaggaat cccatggtta aaaaatcact gcgtcagttc acgctgatgg
      181 cgacggcaac cgtcacgctg ttgttaggaa gtgtgccgct gtatgcgcaa acggcggacg
      241 tacagcaaaa acttgccgaa ttagagcggc agtcgggagg aagactgggt gtggcattga
      301 ttaacacagc agataattcg caaatacttt atcgtgctga tgagcgcttt gcgatgtgca
      361 gcaccagtaa agtgatggcc gtggccgcgg tgctgaagaa aagtgaaagc gaaccgaatc
      421 tgttaaatca gcgagttgag atcaaaaaat ctgacttggt taactataat ccgattgcgg
      481 aaaagcacgt cgatgggacg atgtcactgg ctgagcttag cgcggccgcg ctacagtaca
      541 gcgataacgt ggcgatgaat aagctgattt ctcacgttgg cggcccggct agcgtcaccg
      601 cgttcgcccg acagctggga gacgaaacgt tccgtctcga ccgtaccgag ccgacgttaa
      661 acaccgccat tccgggcgat ccgcgtgata ccacttcacc tcgggcaatg gcgcaaactc
      721 tgcgtaatct gacgctgggt aaagcattgg gtgacagcca acgggcgcag ctggtgacat
      781 ggatgaaagg caataccacc ggtgcagcga gcattcaggc tggactgcct gcttcctggg
      841 ttgtggggga taaaaccggc agcggtgact atggcaccac caacgatatc gcggtgatct
      901 ggccaaaaga tcgtgcgccg ctgattctgg tcacttactt cacccagcct caacctaagg
      961 cagaaagccg tcgcgatgta ttagcgtcgg cggctaaaat cgtcaccaac ggtttgtaat
     1021 agcggaaacg gaatggggaa actcattccg tttttgttta tcgccttaga cggcaaaagc
     1081 gctgtcgccc acctgcgctt gcgcatacca ggccataagc tccgtggttc ctggttctcc
     1141 ttccgctggc gcccagtgcg catagtcatc ggcagccacg ggttgatagc caccgtgttt
     1201 tacttcaaaa attatgccac cggtatccag cgacagcacg gcatgccagg ttcctgcggc
     1261 catctccagc accgtacagg tttcccccaa tatcgcccga tgggtgacgg taccccgatc
     1321 gtcaaaattc agcaccacga aacgacccct taatggcaac agtagttcga aggtgtgagg
     1381 gtgtcggtgc gggcgcacgt aggttcctgg tagcaaattg aagacggagc gagagtagag
     1441 ccacgagccc cgcaaacacg gccgatacaa cgaggccagg gagcggacca gcaaggtcga
     1501 caaacgcgcc ggccgcaagc ataaccatgg gcgaggctga cagcatcacc gccgagaccg
     1561 tgccgagtac ccggccgaga agttctggcg gcgtgcggtt gtagatggca gcgttgagaa
     1621 tgggagagac tgagccggtc agcagtccca cgagcgcgcc caacaacatc agcaccggca
     1681 cgcctggcaa ctgtgaaagc agaagcgagc ccaccgcaga gccacaaaat gccaccgcca
     1741 gccagttctg cgctgatatc cgggcgccga ccgacgcatg aatggcaatg ccaaggagac
     1801 caccagcccc catcattgag gagaacagcc cgagctctgc tacttggcgt cctgcatcta
     1861 caaacagcgc aggcatgatg acgctgccgt tggcgccaac gatgcccacg aagatcatca
     1921 ctataccaaa gagagggcgc agcaggggtt cgctccagag aaaagcgacg ccggcgcgca
     1981 tggagagagt cgccgtcgtg gtcatcgtcc gagcggcacg cgcgggaagc acccacgcgc
     2041 cgagcagacc tgcaaggacg gagcagaacg ccgtcagccc gagcgttggc gcagcgccaa
     2101 gcaggccgat tgcggccccc ccaagggccg ggccacctag aatcgcgacg ttcccgatca
     2161 ccgctttcag tgacgagacg cgctcaacgg agagcccggc gacgtggccg agtttgggca
     2221 gctcactgtc ctgcgcggcc ataccgggtg cgtcgaacgc ggcaccgagc accacgcaag
     2281 cgatcagccc agtgttcgag agggcgccaa cggcatcgag cagtgggatg ctcgccatgg
     2341 ccacgccgcc caccacaccc gagatcaatg cgacgggcgc gcgcccgaac cgatcgacga
     2401 ggccaccacc aacccacgcg ccgatgatgg tcgcgatgac gctgctagcg gccgtggcgc
     2461 ccgcccaggc cgcgctcttt gtatgagaca ggacgaacca tggaagcgcg agggccgcca
     2521 ccgcgttgcc gatccggaag agaaaggtcg ccgcgaacag cgtcgcgagc gggctatatc
     2581 gacgttcgct cattccgctg cggcgagctg cgccttcgcc gcagcgaggt actcttcgtt
     2641 acccgagtcg agggcgaaga gtgcgtaggt gaccgccccg aacgcaaggc gctccgcgat
     2701 gtggtgggcg agccgcggcc acacccggcc accggccgct tcatacgtga ggaggagctt
     2761 cgcgagcccc tcttcaccaa agaccataag gtgcgcggcc atgtcgatgg cagggtcatc
     2821 aacgcgggcc tcgctccagt cgatcatccc gctgacgcgc tccgtgttgt cgatgagcac
     2881 atggcccacg tagagatcgc catgcaccac cacggagaaa tctggccacg acgaatcgtc
     2941 gtcgagccag cgctgccacc ggtggaggcg cttgtcgttc accacgaact cgcgtcggac
     3001 gcggtcaacg tcgtcggcca ccttctgacg ggcctgcgtc ggtgtacgga tgagcatccc
     3061 cgcatccacg gcggcggaaa tggggacggc atgcagggcg gcgagcgcgg tcgcgaagct
     3121 ctccgcgaag acctccgagt cctgcggcac gacccagtcg ggcgtggacg aaccaggctg
     3181 gatgaccatc gcagtcgagt cttcgagcat gggataggca acgagctcgg cgttggccac
     3241 gcgccagtcc ggcaccgcga acggcaggcg attcttgagc attgccagca cccgcgcctc
     3301 tggttcgacc ttcgcgctta cctcggctcg gcgcgggatg cgcagcaccc accgacgtcc
     3361 atcgtcgacg gtggcgatca cgatcctata gtcgagccca agctcattga cagtcagcgg
     3421 gccatggagc ttgagcccat gtcgggctgc aagtgcgtac agttgggagg tatcggcggt
     3481 cgtgactacg gtcatgattc actcctgagg gcttgacggg tttagccacc taaatgta
//
LOCUS       DAPHBJ010000279         3529 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.282, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000279 DAPHBJ010000000
VERSION     DAPHBJ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.282"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..312)
                     /gene="cas5e"
                     /locus_tag="RHZ76_004326"
     CDS             complement(<1..312)
                     /gene="cas5e"
                     /locus_tag="RHZ76_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HDV2814836.1"
                     /translation="MRSYLILRLAGPMQAWGQPTFEGTRPTGRFPTRSGLLGLLGACL
                     GIQRDDTSSLQALSESVQFAVRCDELILDDRRVSVTGLRDYHTVLGAREDYRGLKSHE
                     TI"
     gene            complement(315..1406)
                     /gene="cas7e"
                     /locus_tag="RHZ76_004327"
     CDS             complement(315..1406)
                     /gene="cas7e"
                     /locus_tag="RHZ76_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HDV2814837.1"
                     /translation="MSNFINIHVLISHSPSCLNRDDMNMQKDAIFGGKRRVRISSQSL
                     KRAMRKSDYYAQNIGESSLRTIHLAQLRDVLRQKLSERFDQKIIDKTLALLSGKSVDE
                     AEKISADAVTPWVVGEIAWFCEQVAKAEADNLDDKKLLKVLKEDIAAIRVNLQQGVDI
                     ALSGRMATSGMMSELGKVDGAMSIAHAITTHQVDSDIDWFTAVDDLQEQGSAHLGTQE
                     FSSGVFYRYANINLAQLQENLGGASREQALEIATHVVHMLATEVPGAKQRTYAAFNPA
                     DMVMVNFSDMPLSMANAFEKAVKANDGFLQPSLQAFNQYWDRVANGYGLNGAAAQFSL
                     SDVDPITGQVQQMPTLEQLKSWVRNNGEA"
     gene            complement(1419..1901)
                     /gene="casB"
                     /locus_tag="RHZ76_004328"
     CDS             complement(1419..1901)
                     /gene="casB"
                     /locus_tag="RHZ76_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HDV2814838.1"
                     /translation="MADEIDAMALYRAWQQLDNGACAQIRRVSEPDELRDIPAFYRLV
                     QPFGWENPRHQQALLRMVFCLSAGKNVIRHQDKKPEQTTGISLGRALANSGRINERRI
                     FQLIRADRTADMVQLRRLLAHAEPVLDWPLMARMLTWWGKRERQQLLEDFVLTTNKNA
                     "
     gene            complement(1894..3402)
                     /gene="casA"
                     /locus_tag="RHZ76_004329"
     CDS             complement(1894..3402)
                     /gene="casA"
                     /locus_tag="RHZ76_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HDV2814839.1"
                     /translation="MNLLIDNWIPVRPRNGGKVQIINLQSLYCSRDQWRLSLPRDDME
                     LAALALLVCIGQIIDPAKDDVEFRHRIMNPLTEDEFQQLIAPWIDMFYLNHAEHPFMQ
                     TKGVKANDVTPMEKLLAGVSGATNCAFVNQPGQGEALCGGCTAIALFNQANQAPGFGG
                     GFKSGLRGGTPITTFVRGIDLRSTVLLNVLTIPRLQKQFPNESHTENQPTWVKPVKPN
                     ESVPASSIGFVRGLFWQPAHIELCDPIGIGKCSCCGQESNLRYTGFLKEKFTFTVNGL
                     WPHPHSPCLVTVKKGEVEEKFLAFTTSAPSWTQISRVVVDKIIQNENGNRVAAVVNQF
                     RNIAPQSPLELIMGGYRNNQASILERRHDVLMFNQGWQQYGNVINEIVTVGLGYKTAL
                     RKALYTFAEGFKNKDFKGAGVSVHETAERHFYRQSELLIPDVLANVNFSQADEVIADL
                     RDKLHQLCEMLFNQSVAPYAHHPKLISTLALARATLYKHLRELKPQGGPSNG"
BASE COUNT          957 a          861 c          722 g          989 t
ORIGIN      
        1 aatcgtttca tgacttttca aaccacggta atcttctcgc gctccaagga ctgtatggta
       61 atcacgcaac cccgttacag acacacgacg atcgtcaaga atgagttcat cgcagcgcac
      121 tgcaaattgc acactctctg ataacgcctg taatgaagaa gtatcatcac gttggatccc
      181 aagacaagcc ccgagtagcc ctaataaccc gcttcgggtc ggaaatcttc cggtaggtcg
      241 cgttccttca aaggtcggct gcccccaggc ttgcattggc ccagcaagtc gcaagatcaa
      301 ataagatctc atgttcacgc ctcgccatta ttacgaaccc aggactttaa ctgttctaaa
      361 gtaggcattt gctgtacctg gccagtaata gggtctacat cagacaagct gaattgcgca
      421 gcggctccgt tcagaccata tccattggca acgcgatccc aatattgatt aaacgcctgt
      481 aaagacggtt gcaaaaagcc atcgttcgct ttcaccgctt tttcaaaagc atttgccata
      541 gaaagtggca tatcggagaa attaaccatt accatatccg cagggttaaa agcggcataa
      601 gtacgctgtt ttgctccagg gacctctgtt gccagcatat gaacaacatg ggttgcaatt
      661 tccagagcct gctccctgga ggcaccgcct aaattttcct gaagttgagc gaggttaata
      721 ttggcataac gataaaaaac acccgatgaa aattcctgtg ttcccagatg tgcagaacct
      781 tgttcctgta aatcatctac ggcggtgaac cagtcaatat cagaatcaac ctgatgggta
      841 gtgatcgcgt gcgcaataga cattgcacca tcaacttttc ccaactcact catcatgccg
      901 ctggttgcca ttcttccact aagcgcaata tcaacaccct gctgtaaatt cacacgtatg
      961 gcggcaatat cttccttaag aactttgagc agctttttat catccagatt atcagcctct
     1021 gcttttgcaa cctgctcaca gaaccaggct atttccccca caacccaggg agtaaccgca
     1081 tcggcagaaa tcttttcggc ttcatcaact gatttaccgg agagcagcgc taatgtctta
     1141 tcgatgattt tttggtcaaa acgttcacta agtttttgcc gaagaacatc acgtaattgt
     1201 gcaagatgaa ttgttctgag actggattca ccaatatttt gtgcgtaata atcactttta
     1261 cgcatcgcac gtttaaggct ttgacttgaa attcttactc gtcttttccc gccgaaaata
     1321 gcgtctttct gcatgttcat atcgtcgcgg ttcagacatg aagggctgtg agagatcaga
     1381 acatgaatat tgataaagtt agacatagaa aggtttcctt acgcattttt gtttgtggtc
     1441 aatacaaaat cttccagaag ttgctggcgt tcgcgctttc cccaccaggt caacatcctg
     1501 gccattaatg gccagtcaag tacgggttcg gcgtgagcaa gcaatcgacg taactggacc
     1561 atatcggctg ttctgtcagc ccgaattaat tgaaagatac ggcgctcgtt aattcttcca
     1621 ctattggcta aagctcttcc caacgagata cctgttgttt gctccggttt tttgtcctga
     1681 tgtcggatga cattctttcc tgcgctcagg caaaacacca tgcgtaaaag agcctgctgg
     1741 tgacgtgggt tttcccaacc aaaaggttgc accagcctat aaaacgcagg gatatcgcgt
     1801 aattcatcag gttctgaaac acgtctaatt tgcgcacatg ctccgttatc cagttgttgc
     1861 caggctcgat ataaagccat tgcatcaatt tcatcagcca tttgatggcc ctccttgcgg
     1921 ttttaactcc cgtaaatgtt tgtatagcgt ggcgcgggcg agcgctaatg tgcttattaa
     1981 tttaggatga tgtgcatagg gcgctacaga ttgattaaat aacatttcac acaattgatg
     2041 aagtttgtct cgtaaatcag ctatgacctc atcagcctgg gaaaaattaa cattcgccag
     2101 tacatcggga attaataatt cactctgtcg atagaaatgc ctttctgcag tctcatgaac
     2161 agagactcca gcccctttaa agtctttatt tttaaaccct tctgcaaagg tatataacgc
     2221 cttgcgtaag gctgttttat atcccaaacc aacagtaact atttcgttta tcacattgcc
     2281 gtattgttgc cacccctgat taaacatcaa cacatcatga cgccgttcaa gaatagatgc
     2341 ttgattatta cgatatcccc ccataatcaa ttcaagagga ctttgcggcg caatatttct
     2401 gaattgattc acaaccgccg ccacgcgatt tccattttca ttttgaataa tcttatctac
     2461 cacaactcgg ctgatttgtg tccatgatgg tgcggaggtg gtgaaagcaa gaaatttttc
     2521 ctcaacctcc cctttcttga ctgttaccag acaaggggaa tgcggatggg gccatagccc
     2581 attaactgta aaggtaaatt tttccttaag aaaaccggta taacgcaaat tgctttcctg
     2641 tccacagcaa gaacattttc caatcccaat gggatcgcat aattcaatat gcgcaggttg
     2701 ccagaataag ccgcggacaa agccaattga cgaagctggc acagattcat tgggtttgac
     2761 aggcttaacc caggtaggtt ggttttccgt atgtgattca ttgggaaatt gtttttgaag
     2821 acgaggtatt gtgaggacat tgagtaacac cgtcgagcga agatcgatcc cacgtacgaa
     2881 ggtggttata ggtgttcctc cacgtaaacc gcttttaaag ccaccaccaa aaccaggtgc
     2941 ctgattcgcc tggttgaata acgcaatcgc agtgcatcca ccacataatg cttcaccctg
     3001 acccggttga ttgacaaaag cacaattcgt cgcgccgctt acccctgcca acagtttttc
     3061 cattggagtc acatcatttg ctttgacacc tttggtctgc ataaagggat gttctgcgtg
     3121 attaaggtag aacatatcta tccacggcgc gatgagttgt tgaaactcat cttcagtgag
     3181 cggattcatt atgcgatgtc gaaattcaac gtcatctttt gccgggtcga taatttgccc
     3241 aatgcaaacc agcagtgcta aagcggccag ttccatatcg tcacggggca aacttaatcg
     3301 ccactgatct ctactgcagt atagcgattg cagatttatg atttggactt tccccccgtt
     3361 tcgcgggcgt acagggatcc agttatcaat aagcaaattc atttgttctc cttcatatgc
     3421 tccgacattt ctcctgcatt tctatacgtc ggcacacttc cgttatcggt aaaaacgaag
     3481 aaaaaatacg gcgtaattaa ttcaataatc acattcactg caaaaatat
//
LOCUS       DAPHBJ010000280         3526 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.283, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000280 DAPHBJ010000000
VERSION     DAPHBJ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.283"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(84..260)
                     /locus_tag="RHZ76_004330"
     CDS             complement(84..260)
                     /locus_tag="RHZ76_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814840.1"
                     /translation="MSKSSPVLPEDRDDIEFVMDDFQRSLGKSKPVREVQFLGESQQS
                     EESANSADSPWFES"
     gene            complement(275..1951)
                     /locus_tag="RHZ76_004331"
     CDS             complement(275..1951)
                     /locus_tag="RHZ76_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDV2814841.1"
                     /translation="MSAVESDSLPMGAPGSSLVAYMPHTGLLINDDNFLPEMREELDV
                     INRQVESYNDELSLLRSMSAQFLKCGNQKLYSYLSGYNHLISEADASYAENALRSEYW
                     KRVMALTDVLSVMSDEKKKEWDKQFTADRYHLPPQEIPDFTLDAVVTTVVALLNDRHQ
                     FIKDRVYAVFQSLSRQHKTNKAFGFSTRMITSGVCEGVKDKWVKLKVEFKESGLMPLS
                     ELRVICSYFRGETVKPVYDTKKMVENMVGHVGFRNWISLDGNSIRFRVYKNGSMHIDV
                     HPDIAERLNNILAAIVPLALPAERVAHTKATLQEFPALKRCIDFHSRMQLAELDFTQN
                     EKEWSSWTSLGYAGENADKTRQVNADVLRFLGARVTSYHVTFDYDPTEVMRFIGHMGA
                     MPDIKSHQFYPSSARLSEYVASIIAAGEGERLLEPNIGHGDLLQCFPKSVNVTGIELD
                     TLNCLVSRAKGYETTEADFLAWSAANQIQKFDCVVMNPPFADNRAKLHLLAAASHLVP
                     GGRLAAVLPLSLQGIDNLLGEGFRTEWMDTFDNEFDNTAVSVRVLYAERF"
     gene            complement(2215..3372)
                     /locus_tag="RHZ76_004332"
     CDS             complement(2215..3372)
                     /locus_tag="RHZ76_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDV2814842.1"
                     /translation="MSDLMTDSQQQTNSRVFKSPLVSFSTVTGSGRFVSGMKQFRDAN
                     PDLCNEVTEQKAWAISPTVVQITEGFNSREMGMGEAYYQLPEVADHLNNIKNAYIRGD
                     YVDPIRVLIKDGIPYVRQGHCRLKAALMAVAEGHSNLTILCIEMKADEISCELTTIDG
                     NRGLALSPVALGESYRRLQALGGWSLDRIAEREGKSNTTIAALIRLTGCCVYLKKLIH
                     ADAISYVAVLSLIDEFGETEAVARIEKMIADLEKADSSGIQIKKTPRGQIRVVPSDFK
                     PARVPPVLATKAVEGVKLITTSLLQKLGDIELPEVTDSSEDEEITFTLNRSMLEMLKN
                     LQSEITESENKQLRRAENRQARLKGEKVTYKTKKNDKNGGEPQSETPPAQA"
BASE COUNT          906 a          876 c          842 g          902 t
ORIGIN      
        1 gttcagtaca ggtaaaagtt gtaataactc cccgctcact gccggggagt cattttaaat
       61 cagcacggcg ccatcagaat agattagctt tcaaaccagg gcgaatcggc agagttcgca
      121 gattcttcac tttgctgtga ctctcccaga aattgcactt cacgtactgg tttacttttc
      181 cccaggctcc gctggaaatc atccatcaca aactcaatat catcacggtc ctcaggcaag
      241 acaggcgaac ttttggacat agtctttcct tcagttaaaa gcgttctgca tacagaacgc
      301 ggacggagac ggcagtgttg tcaaactcat tatcgaaggt atccatccac tcggtcctga
      361 acccctcgcc cagcaggtta tcgatcccct gaagggacag aggcaggacg gctgctaaac
      421 gcccgccggg aaccagatga gacgcagcgg ccagcagatg cagcttagcc cggttgtcag
      481 caaaaggagg gttcatcacc acgcaatcaa atttctgaat ctgattcgcc gccgaccagg
      541 ccagaaaatc ggcttccgtg gtctcgtaac ctttagcccg ggagaccaga caattcagtg
      601 tatccagttc gatgccggtt acgttgacac ttttcgggaa acattgaagc aaatccccgt
      661 gcccaatatt gggctccagc agccgttccc cctccccggc cgcaataatg gatgcgacgt
      721 attcactgag tcgcgccgac gacggataaa actggtggga tttaatatca ggcatggcgc
      781 ccatatgtcc gatgaagcgc atcacttcgg tcgggtcata atcaaacgtc acatggtagc
      841 ttgtcactcg ggcaccgaga aatcgcagaa catccgcgtt cacctgccgc gttttatcag
      901 cgttctcacc ggcatagccg agcgatgtcc agctggacca ttccttttca ttctgggtaa
      961 aatccagctc tgcaagctgc atccgtgaat gaaaatcgat gcagcgtttc agtgccggga
     1021 actcctgcaa cgtcgctttg gtatgggcca cgcgttcagc aggcagagcc agagggacga
     1081 tggcagcaag aatgttgtta agccgttcgg cgatatcagg atgcacatca atgtgcatcg
     1141 atccgttttt gtagacccga aaacgaatac tgttgccatc caggctgatc cagtttctga
     1201 atccaacatg accaaccata ttctcaacca tttttttcgt gtcatagacc ggtttaactg
     1261 tttcgccccg gaaataagaa cagatcaccc gcaactccga aagtggcatg aggccggatt
     1321 ctttaaactc aaccttcagt tttacccatt tgtctttcac gccttcacat acgccggagg
     1381 tgatcatgcg agtagagaag ccaaatgctt tattggtttt gtgttgacgg cttaatgact
     1441 gaaagacggc atagacacga tctttaatga actgatgccg gtcattgagc aacgcgacaa
     1501 ccgtggtaac aacagcatcg agtgtaaaat cagggatctc ctgaggagga aggtggtaac
     1561 ggtcagcagt aaactgctta tcccattctt tcttcttttc gtcagacatc acagacagta
     1621 catcggtcag ggccatgacc ctcttccagt attctgaacg cagcgcgttt tctgcgtagc
     1681 tggcatccgc ttctgaaatg agatgattgt atccggagag gtatgaatag agcttctgat
     1741 tcccgcattt caggaactga gcggacatgg acctcagtaa gctaagctca tcgttatatg
     1801 attcgacctg cctgttaatg acatcgagct cttcccgcat ttcgggcagg aagttatcgt
     1861 cgttaataag gaggccagta tgaggcatat aagcaaccag agaactccca ggggcaccca
     1921 ttggcaggga atcagattca acagcagaca tagcaccagt cctttttgaa gggtttatct
     1981 gataccagga taacaacctt gaacaaaaag ctaataggtt ggctgacatg aaaatggaga
     2041 aattaacact gcggaaagtc agaaagacag atgtggtttt aatcagaaga aacgggccgg
     2101 agacaggaca aaaaaacccc tacatagtag gggcaacggg gggagagcct gggtggctcg
     2161 ctctaaaata tcgtacttct tattgtagtc ttctcacact ctgaaataat tcatttaagc
     2221 ctgtgctggg ggtgtttcgg attgcggttc tccgccattt ttatcgtttt tcttggtttt
     2281 gtaagtcact ttttcccctt tcaatcgggc ttgccgattt tcggcccggc gaagctgctt
     2341 gttttctgat tccgtaatct cactttgcag gttcttgagc atctccagca tgctccggtt
     2401 cagcgtaaag gtaatttctt catcttcgct ggagtcggtc acctccggca actcgatatc
     2461 accgagtttc tgcaatagac tcgtcgtaat gagtttgacc ccttcgacgg cttttgtagc
     2521 gagaactggt ggaacacgtg cgggtttaaa atcggaaggg acaacacgga tctgcccgcg
     2581 tggcgttttc ttaatctgga taccgcttga atcagccttt tcaaggtctg cgatcatctt
     2641 ttcgatccgc gcaacagctt ccgtttcacc gaactcatcg ataagggaca ggactgctac
     2701 ataggaaatg gcatcggcat ggatcagttt tttgagatag acacagcagc cggttaaacg
     2761 aataagagca gcaattgtgg tattactttt tccctcacgt tcggcaatac ggtcaagcga
     2821 ccatccaccc agcgcctgaa gacgtcggta ggattcaccc aaagccacag gagaaagagc
     2881 caggccacgg tttccgtcaa tcgttgtcaa ttcgcagctg atctcatcgg ctttcatctc
     2941 gatgcaaaga atggtgaggt tagaatgccc ttctgccaca gccatcaaag cagcctttaa
     3001 acggcaatgt ccctggcgaa cgtacggaat gccgtctttg atcagaacgc ggatagggtc
     3061 tacgtaatca ccccggatgt acgcattctt gatgttattg agatgatcgg cgacttctgg
     3121 caactggtaa taagcttccc ccattcccat ctcacgggag ttgaatcctt ccgtgatttg
     3181 aaccacggtt gggctgatag cccatgcttt ctgttcagtg acttcattac aaaggtcagg
     3241 gttcgcgtcg cggaactgct tcataccact gacgaatcgg ccggaaccgg tcactgttga
     3301 aaaagacacg agtggggatt taaagacccg tgagttagtt tgctgttggg aatcagtcat
     3361 cagatcactc atagatactc cgttgaattc ttttttgtca tacctttcat ctgtgcgtga
     3421 atactaccgg aaaaaaaaat ctcacttttt cgttaaaaag gtactttttg ggtctattcc
     3481 gaattgaacg aggaaaattg ttctggcgat cagaacaaaa agaaaa
//
LOCUS       DAPHBJ010000281         3510 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.284, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000281 DAPHBJ010000000
VERSION     DAPHBJ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.284"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            99..1073
                     /locus_tag="RHZ76_004333"
                     /pseudo
     CDS             99..1073
                     /locus_tag="RHZ76_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612075.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2166..2495
                     /locus_tag="RHZ76_004334"
     CDS             2166..2495
                     /locus_tag="RHZ76_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001647051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV2814843.1"
                     /translation="MFSVVYHPEAREEATALPVKIRVKFDRLIGKLEYDARLLREPDT
                     KPLGDGLFEIRTMGTDIARGIWVYHKGNTIIMLRVFIKKSQKTPAKEIDLAKKRLAEV
                     LNETGKP"
     gene            2476..2757
                     /locus_tag="RHZ76_004335"
     CDS             2476..2757
                     /locus_tag="RHZ76_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2814844.1"
                     /translation="MKLVSHKELHNEWMQDDEYRAAWQEEERKEKLRELLAEWRKHDN
                     LTKAQVAERMGVTPPVISRLENNITKASIDTLTRYAHACGIKKPVIALY"
     gene            3035..3510
                     /locus_tag="RHZ76_004336"
                     /pseudo
     CDS             3035..3510
                     /locus_tag="RHZ76_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          945 a          884 c          863 g          818 t
ORIGIN      
        1 aaaaataacc ttaacgaatg gaagggactt cccctggata tgcgcctgat taactcttgt
       61 gcaatcatgg ggtaaacata gccccggagg gattcgttat gaccatcacg actgtcggta
      121 tcgatcttgc taaaaatgtg ttcgctgttc actgcgttga ccaacacggt aaaactgttc
      181 tggttaaacc caaagtatcg cgtgctgccc ttcctgagct gattgccagt ttacctccct
      241 gtgttatcgg gatggaggca tgctccgggg ctcactactg ggcaaggttg tttcggcaat
      301 atggtcatga accacggctg atggctgcta aatttgtatc tccttaccgc atggccggta
      361 aatcaggcaa aaatgatgca gctgatgctc agtctatctg tgaagcagtt cgtcgcccgc
      421 atatgcggtt tgtaccagtg aaggatgaga gccagcaggc gatgcaatgc ctacaccgta
      481 tcagacaggg ttttatcgaa gaggaaacag caacatataa ccgattgaga ggtttgatat
      541 cggaattcgg tgtcatcgcc ccacaaagta cagatgccct gcgtcacatg gtttctgagc
      601 agaagagttc tttgccactt caggttcaac aatgtgttaa tgacctgctg gaacacattg
      661 atcgtattga agataatatt actgaatatg accggatttt gtcccgcatg gcaaaaacag
      721 atcaccgcag ccagcgattg atggaactga aagggattgg tcccacaacg gcctgtgcgc
      781 tggtcgccag tatcggtaat gcacacgatt ttaaaaatgg gcgacaactg gcagcctggc
      841 tgggactaac gccatcgcag tacagcagtg gtgggaaatc aaagcttggc aggataacga
      901 aagctggcga ttcgtatctg cgaacgctac tggttcaggg ggctcgttcg gttctgattg
      961 gtgccgagaa aaggactgat ttattcagtc gttgggtctg ttcactggtc gatcgcagag
     1021 gatactggcg tgccgttgta gccatcgccg ccaaaaacgc aaggctgtgc tggcatcatt
     1081 gcattacggt gatgatttca ggctgtactc agccagctaa agcacgaaac aaaataacca
     1141 tctgacctgc aattcgttga tgataaaggg ttagaccccg tgaggcctat ctggctattg
     1201 tacaggatat acgtatccgc ttaacgaaca agaacctcac gcgcgtcttt catcagggtc
     1261 cgaatcgatg acgattcatc atggccgttt atagtaccgc agtctctccc ctttattttt
     1321 actgaagagc agacagaaac cataaacacc ggcgttgaca cgccggggaa gcccttatag
     1381 tacaataatt ttcgaaatcc aacccatccc gcctacagga atgacggatt tttcggcggt
     1441 tccggcgtag acccaccaca actgctgtat agcttgtggg taagtgataa tgaagtgcaa
     1501 acgagatggc gcaaatactc ttttggttta tcttttaatt acttacgtat taaaagataa
     1561 accaaaaaat aaaagacatt actgttatcc cctttaaaaa gtggataacc gaacagcatc
     1621 ggcgcgagcg cctcaaaagt agctccccac gctaaagcgc ggggcattca gaacggaatc
     1681 cctcgatggc taacagctcg cctgttagcc atcgagggat tggacaaatc cggccacgcc
     1741 ggatacgtca gcaaatatcg ctgaagaatt agcttacacg ctcgtaagcg gctcttacga
     1801 cccgctaacg cggagatacg ccccaactgc ggcttcgcct tgttgtgacc actccgaccg
     1861 cgcacaaaag cctctgagcc gcttttagcg gaatatggct ttgcaaggtg aagggatgga
     1921 taggcgatcc ctttcgatcc ctcactggta tcggcggcta accctcggtg ctacctgtca
     1981 tttcatcctg ccgccttaca tgcgctaacg cgcataaatc cccgttcctc agtggaacga
     2041 aaactcacgc taacagggta gtgaccgggt agccacgata actgccaaca aaattagctt
     2101 ataggttaat tctaccttgt ctgaaattag cctataggtt aatattaata gaaatgggag
     2161 gtcacatgtt tagcgttgtt tatcacccgg aagccaggga agaagccaca gcattacccg
     2221 taaaaatcag ggtgaagttt gaccgtctca tcggcaaact ggagtatgac gcccgattac
     2281 tgcgggagcc ggacacgaag cccctgggcg atggactttt tgagatccgc acgatgggga
     2341 cggacatagc caggggaatc tgggtatacc acaaaggcaa taccatcatc atgctccgtg
     2401 ttttcatcaa gaagtcacag aaaacgccag cgaaagaaat cgatctcgcc aaaaagcggt
     2461 tagcggaggt acttaatgaa actggtaagc cataaagaac tgcataacga gtggatgcag
     2521 gacgatgaat atcgcgctgc atggcaggaa gaagaacgta aggaaaagct cagggagctg
     2581 cttgctgaat ggcgcaagca tgacaacctg acaaaagcgc aggttgctga acgcatgggg
     2641 gttacacctc cggttatttc tcgcctggaa aacaacatca ccaaagccag catcgacaca
     2701 ctgacccgct acgctcacgc ctgcggcatc aagaaacctg tcattgccct gtactgaata
     2761 gccagccctc cggggctggc ctttttgttt cggtcagtcc caaaattttg ttaaagcaag
     2821 tttaaaatgt cacctgtttc tccaaagttg aaatgtcact tctaagccaa tctatttata
     2881 cataatcttt acatctttcc ccggtgacaa agtgctaatt ggtctgtaat gttcaatgtg
     2941 tggcaggcaa gccgccggtg acttagggaa ggtgcgaaca agttcctgat atgagatcat
     3001 catattcatc cggagcgcat cccagaggga catcatgagc catcaactca ccttcgccga
     3061 tagtgaattc agcactaagc gccgtcagac ctgaaaagag attttcctct cccgcatgga
     3121 gcagattctg ccatggcaga atatgaccgc tgtcatcgag ccgttttatc ccaaggcggg
     3181 caatggccga cggccctatc cgctggaaac catgctacgc attcactgca tgcagcattg
     3241 gtacaacctg agcgatggcg cgatagaaga tgctctgtac gaaatcgcct ccatgcgcct
     3301 gtttgcccgg ttatccctgg atagcgcctt gccggaccgc accaccatca tgaatttccg
     3361 ccacctgctg gagcagcatc aactggcccg ccaattgttc aagaccatca atcgctggct
     3421 ggccgaagca ggcgtcatga tgactcgagg caccttggtc gatgccacca tcattgaggc
     3481 acccagctcg accaagaaca aagagcagca
//
LOCUS       DAPHBJ010000282         3387 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.285, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000282 DAPHBJ010000000
VERSION     DAPHBJ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.285"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..130
                     /locus_tag="RHZ76_004337"
     CDS             <1..130
                     /locus_tag="RHZ76_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814845.1"
                     /translation="IIPIITATLILNYLNNSERSLVKQILIEDEIIVCATYLIPDI"
     gene            132..476
                     /locus_tag="RHZ76_004338"
     CDS             132..476
                     /locus_tag="RHZ76_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HDV2814846.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            480..815
                     /locus_tag="RHZ76_004339"
     CDS             480..815
                     /locus_tag="RHZ76_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HDV2814847.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(872..3114)
                     /locus_tag="RHZ76_004340"
                     /pseudo
     CDS             complement(872..3114)
                     /locus_tag="RHZ76_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          895 a          791 c          709 g          992 t
ORIGIN      
        1 cataattcct atcatcacag ccacgctcat tttgaattat ctgaataata gcgagcgttc
       61 tttagttaag cagatcttaa ttgaagacga aattattgtg tgtgcaactt acttaatacc
      121 ggacatctaa gttgaagaaa aaaatacttg ccttcgggct tatctcagca ttgttctgct
      181 ccacgccagc gatggcggat atgaaccgca ccacgaaagg tgcattgcta ggcgcaggcg
      241 ttggtttact tactggcaat ggtgttaatg gcgtactgaa aggtgcagct gttggcgctg
      301 gtgttggtgc agtaacagaa aaaggccgcg acggtaaaaa tgcgcgtaaa ggtgcaaaag
      361 tgggtgccgc cgttggtgct gtcactggcg ttctgaccgg caatggcctc gaaggggcga
      421 ttaaaggtgc cgtgattggc ggtacgggtg gtgcaattct gggtaaaatg aaataaggaa
      481 tgatgatgaa aaaatttgct ttactggcgg gcctgtttgt tttcgcacca atgacctggg
      541 cgcaggacta caatattaaa aacggtttgc cgtcagaaac ctatattact tgcgcagaag
      601 ccaacgaaat ggcaaaaact gacagcgcac aggttgcaga aattgttgcg gtaatgggta
      661 atgccagcgt tgccagccgt gatttaaaaa ttgagcaatc tccggaactg agcgccaaag
      721 tggtagaaaa actgaatcag gtttgcgcta aagacccgca aatgctgttg atcacggcaa
      781 ttgatgacac catgcgtgct atcggcaaaa aataatattt catatgtacc gcgtcgatga
      841 atacgacgcg gttttttagc taacggcggg atcagaactt atatttcagg cccaccatca
      901 ctgcggtgtc attgtagcca ttatcaccca gctgcacgcc cacattcccc cagacgctgg
      961 ccgctggatt caggttgcca tttacgccaa gtttaatttc ccccaaatta ctcgccccat
     1021 cttgttcgac tttcacaccg ttcattgaga cagcaaaatc tttactgtta tggatccagt
     1081 tcatttcaac aaaaggttca aagtcgtcac cgtgcggacc gctgtttttc tcctgagtac
     1141 gaataaaggt gcggaaaccg agtcgggtct ggatgttgtt atttgcgtca ttctcaatac
     1201 gtgagccatt ggcttccgtg tgcgcattat gatcgacgcc catccagata gcctgtgcct
     1261 gtggctggaa gatccacgta tatttcgccg cttccagacc aaaggattta ttcagggcaa
     1321 aaccgtaacc cgattccagc gagacggttg cacctttcgc gctataggat tcataggcta
     1381 attcatcccc tttcaccgag tttttaaacc agctatattg cgcccaggag tcaatgtatg
     1441 cgcctttttt actgatgtca tcggcaaacc aggtggcata cagccctgcg ctatagcctc
     1501 tgacactccc tttcgaacga taatccgaca cgctggaatg agttaagttg tagtcgcggg
     1561 cataaccagc catcactccc aaacgccaac tgtcactatc ggtaaaacca ccggttaaca
     1621 gatcgccccc taactgcgag acgtagcgat gcgaggtcgt tctcaactgt ccgttgctgt
     1681 cacgccaggc attatgcccg ccaatttgac gtagccaaag tcggcttgaa cgctcctgtt
     1741 cagttacagg atcgatgtag cgcgtttcac ccgcacggtc gttcagacgc ataacaaaaa
     1801 gagtgttagc agctgccagg ttagcgacgt aacttccagc ttcggggcgc aggacggggg
     1861 taggcgtttc tgttgattct gaatttccct ctgagctgtt atcctcctca ttcactgact
     1921 gataacttgt caaataccag tttttgcctt gttgttttag ttgatattcg aaagctccag
     1981 ctgcaatcct tgcatcacct tgtctgaagg catctgctgg agattcatcg ccattcacag
     2041 ttatgagttc gatcccgttt agcgtggccg cgccgcttcc tccagcattt ttcacataga
     2101 cggtagttga acccgaagta ttaccctcaa caataagtct gtccgtagcc gatttatcat
     2161 cacctaatac ggtgcggata ttaagctgac cattattacc gacataatta ccttttacgg
     2221 ttaatgtttt ccttgggtaa atgtttgacc atcatcatac gaaagattga taatactgtc
     2281 tgaattagtc agatgggtaa caacggagtc atctttcata ttccagattg tattttctga
     2341 aagcttcagc gtggttgtgc cgctgacgag tttagtcgct ccatttagaa tagagtcgga
     2401 cgaaattatg tttaagtcac ctccagtatt attaaccaga acattattac caggtgtgat
     2461 aatacttcga ttaaggatac ttacatccgt tgtagttttg ttaacatcaa tactatccaa
     2521 agcacttgaa agttcaacac ttaatagtgc tattttggct tgagaatcga tatttgatgc
     2581 atcaattgat attcctttat cttgttttga taccgcggtg acattggaaa aaaataactc
     2641 tccaccattg tatatttttg caatgggggc tttagtgcct gttgttgtaa tagaactatt
     2701 ttttatatca atgttacctt gtcgaatagc aactgcataa cttccatcag ccacagtttc
     2761 aagtgccaca taatctaaat taatatatgc ttttttccca ttagcataag caccatagct
     2821 attaatgcca ttagttgtta tggaaccacc attcattttt aaatcagcac ggctaataac
     2881 ttcgtaactg ttagaatcac taacttcatc atctacagca tcgacacatg ttcgagactc
     2941 tccaggactt gaacatgcaa gaacacctac tgcattttcc ccagaggtaa caatgttcgt
     3001 tttatctgtg gtttcaagac gggtgttatt taccatcttt tcagaatcat taacctggct
     3061 taaaagtccc gctgaatatg ccccacccga ctcgactgaa ttatcaccat tcataaagat
     3121 ggtgccacca aattcactga ctactgcgct ggctgttatc cctccatttt ctttgtcaga
     3181 tacgcttcca gaggtcttaa tatcgctatc gcttatatat aatactttgt tttgactatc
     3241 aacagaaata ccataagatc catcaccact agtcatcaca ctggcattat ttatatatcc
     3301 agttacaccg tcatccactt tcacagcatg gctttcgtct tcagtggtta gatactctcc
     3361 attacttatc ggattattat ctttgat
//
LOCUS       DAPHBJ010000283         3384 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.286, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000283 DAPHBJ010000000
VERSION     DAPHBJ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.286"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            138..1103
                     /gene="dusB"
                     /locus_tag="RHZ76_004341"
     CDS             138..1103
                     /gene="dusB"
                     /locus_tag="RHZ76_004341"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDV2814848.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1129..1425
                     /gene="fis"
                     /locus_tag="RHZ76_004342"
     CDS             1129..1425
                     /gene="fis"
                     /locus_tag="RHZ76_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HDV2814849.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1511..2395
                     /gene="yhdJ"
                     /locus_tag="RHZ76_004343"
     CDS             1511..2395
                     /gene="yhdJ"
                     /locus_tag="RHZ76_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDV2814850.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NRSKRCRVSEVDPDLIAK"
     gene            2479..2658
                     /locus_tag="RHZ76_004344"
     CDS             2479..2658
                     /locus_tag="RHZ76_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HDV2814851.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(2661..3323)
                     /gene="acrS"
                     /locus_tag="RHZ76_004345"
     CDS             complement(2661..3323)
                     /gene="acrS"
                     /locus_tag="RHZ76_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HDV2814852.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKTLYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT          923 a          785 c          806 g          870 t
ORIGIN      
        1 attatcgcac cacgctcatt ttaaatgcaa ttctttgatc catctcagag gattggtcaa
       61 agtttggcct ttcatctcgt gcaaaaaatg cgtaatatac gccgccttgc agtcacagta
      121 tggtcatttc ttaactcatg cgcatcggac aatatcagct cagaaatcgc ctgatcgcag
      181 cgcccatggc tggcattaca gacagacctt ttcggacgtt gtgctacgag atgggagccg
      241 gattgacagt atccgagatg atgtcttcta acccacaggt ttgggaaagc gacaaatctc
      301 gtttacggat ggtgcacatt gatgaacccg gtattcgcac cgtgcaaatt gctggtagcg
      361 atccgaaaga aatggcagat gcagcacgta ttaacgtgga aagcggtgcc cagattattg
      421 atatcaatat gggttgcccg gctaaaaaag tgaatcgcaa gctcgcaggt tcagccctct
      481 tgcagtaccc ggatgtcgtt aaatcgatcc ttaccgaggt cgtcaatgca gtggacgttc
      541 ctgttaccct gaagattcgc accggctggg caccggaaca ccgtaactgc gaagagattg
      601 cccaactggc tgaagactgt ggcattcagg ctctgaccat tcatggccgt acacgcgcct
      661 gtttgttcaa tggagaagct gagtacgaca gtattcgggc agttaagcag aaagtttcca
      721 ttccggttat cgcgaatggc gacattactg acccgcttaa agccagagct gtgctcgact
      781 atacaggggc ggatgccctg atgataggcc gcgcagctca gggaagaccc tggatctttc
      841 gggaaatcca gcattatctg gacactgggg agttgctgcc cccgctgcct ttggcagagg
      901 ttaagcgctt gctttgcgcg cacgttcggg aactgcatga cttttatggt ccggcaaaag
      961 ggtaccgaat tgcacgtaaa cacgtttcct ggtatctcca ggaacacgct ccaaatgacc
     1021 agtttcggcg cacattcaac gccattgagg atgccagcga acagctggag gcgttggagg
     1081 catacttcga aaattttgcg taaacagaaa taaagagctg acagaactat gttcgaacaa
     1141 cgcgtaaatt ctgacgtact gaccgtttct accgttaact ctcaggatca ggtaacccaa
     1201 aaacccctgc gtgactcggt taaacaggca ctgaagaact attttgctca actgaatggt
     1261 caggatgtga atgacctcta tgagctggta ctggctgaag tagaacagcc cctgttggac
     1321 atggtgatgc aatacacccg tggtaaccag acccgtgctg cgctgatgat gggcatcaac
     1381 cgtggtacgc tgcgtaaaaa attgaaaaaa tacggcatga actaattcag gttagctaaa
     1441 tgcttgatta aaaaggcgct actcggcatg gggaagcgcc ttttttatag gtgtcacaaa
     1501 gggagtgacc atgagaacag gatgtgaacc gacccggttt ggtaatgaag ctaagaccat
     1561 tattcacggt gatgcccttg ccgaacttaa aaagctacct actgaaagcg tcgatctgat
     1621 ctttgccgac ccaccgtata acatcggtaa aaattttgat ggtctgatcg aagcctggaa
     1681 agaagatctg tttatcgact ggctgtttga agtgattgca gagtgccacc gcgttctgaa
     1741 aaagcagggc agcatgtaca ttatgaacag tacggaaaac atgcccttta tcgatctcca
     1801 gtgccgcaag ctttttacca tcaaaagtcg catcgtctgg tcatatgaca gttctggagt
     1861 acaggcgaaa aaacactacg gctccatgta cgaacccatc ctgatgatgg tgaaagacgc
     1921 aaagaactac acattcaacg gtgatgctat tctggtagaa gccaaaaccg gatcgcagcg
     1981 cgcgttgatc gattatcgca aaaatcctcc acagccatac aatcatcaaa aagtaccggg
     2041 taacgtctgg gattttccgc gcgtgcgtta tttaatggat gaatatgaga accacccgac
     2101 gcaaaaaccg gaagccttac tgaaacgcat tattctcgcc tcttccaacc caggcgatat
     2161 cgttctcgac ccgtttgccg gtagctttac taccggtgcc gtagccatcg ccagcggacg
     2221 aaaattcatt ggtattgaga tcaacagcga gtacatcaaa atggggcttc gacggctgga
     2281 tgtcgcgtcg cattactccg cggaagaact ggcgaaagtg aaaaaaagaa agacgggcaa
     2341 ccggtcaaaa cgatgccggg ttagcgaagt tgaccccgat ctcattgcaa agtaaaggaa
     2401 gtgtaagcct ggttttcaga ttattcattt cgtgtatatt tcctgccaga cttggttaaa
     2461 catgcacagg ctcaggtaat gattcgcaag tattggtggc tcgtcgtttt cgctgtcttc
     2521 gtttttctgt ttgatacttt actgatgcag tggattgaac tgctggcaac agaaacagac
     2581 aaatgccgta atatgaactc agttaatcca ctaaaactgg ttaactgtga cgaactgaat
     2641 tttcaggaca gaatgtgaat ttacatgaca cttaattcat tcgtttgatg aattaatttc
     2701 gttatgtttt catctggcat gaacattctt aatacgttat cgaccagcgc gggtgcttgt
     2761 ttataaagat cataacccgc catattcatt aaccagtttt gaacaattcc gctgaaggca
     2821 ccatcaataa taatcatcac aacatctaaa tcgaggttat ttgctacaca accttgttgc
     2881 tgacacgcct gcaatacttc gcggagagtc tgcggattaa agcccatctt ttcgcgtatc
     2941 actccctcgg ccagcatctc atcattaaat tcacatttgt gatataaggt tttcagcaac
     3001 gcctgctggc ggggaatttt ggcaatatat tgcaagccga caatcaattt ttcacgcaat
     3061 tgttgaaacg ggtcatgctc taatccagcc gtcaagtgtt cctggattaa ctcccgcaat
     3121 gaaggctgtt gcaaccacat ctcattaaac agttgggtct tgttctcgaa gtgccagtag
     3181 atagcgccac gcgtaacgtt agcggcatcg gcaatgtcgt tgagcgtcgt cttgcttacg
     3241 ccatgttgcg caaactgggc gatggcagtt tcaatcagtt cttgccgggt cttcagagct
     3301 tcggctttgg ttctttttgc catgattaat tattcaggaa ataaatatat tcgacacagg
     3361 gtgagaaaat agcgaaggtt aatc
//
LOCUS       DAPHBJ010000284         3281 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.287, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000284 DAPHBJ010000000
VERSION     DAPHBJ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.287"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(58..819)
                     /gene="kduD"
                     /locus_tag="RHZ76_004346"
     CDS             complement(58..819)
                     /gene="kduD"
                     /locus_tag="RHZ76_004346"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HDV2814853.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(849..1685)
                     /gene="kduI"
                     /locus_tag="RHZ76_004347"
     CDS             complement(849..1685)
                     /gene="kduI"
                     /locus_tag="RHZ76_004347"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDV2814854.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTGTPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1972..3153)
                     /locus_tag="RHZ76_004348"
     CDS             complement(1972..3153)
                     /locus_tag="RHZ76_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDV2814855.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
BASE COUNT          875 a          885 c          734 g          787 t
ORIGIN      
        1 tctacaggta taaaaaaccc tgccatgcgg cagggtcata aaagtaagaa gaatgaatta
       61 acgcgccagc caaccgccat ccacggcaat ggtataacca ttcacataat ctgaagcgct
      121 ggaggcaagg aacactatcg gccccatcag gtcactcggc agtccccaac gaccagctgg
      181 aatgcggtcg agaatttccg cgctacgttg ttcatctgcc cgtagttgtt gagtattgtt
      241 ggtcgccatg taacccgggg ctatcgcatt aacattaatg ttgtgtttag cccattcgtt
      301 cgccatcaat cgcgtcacac ccatcacgcc gctttttgat gcggtataag aaggcacacg
      361 gatcccgccc tggaaggaga gcattgacgc gatattgata atcttgccgc cattgccttg
      421 cgcgataaag tgtttcgccg ctgcctgaga catgaagaat acgctcttga tattcaggtt
      481 catgacatcg tcccagtcct tttcgctgaa ctcgagagca tcttcgcggc gaatcaatcc
      541 ggcgttattc accaggatat caatatgacc aaactccgct accgcgcgat ccagcagtgc
      601 tggaatacca tcaatctttc gcagatcggc ggtcaggctt aaaaaacgac gccccagcgc
      661 tgtgacctgc tcgatggttt cagtcggttc aacgatgtta atgccaacaa tgtcacagcc
      721 cgcttgcgcc agccccaacg ccatcccctg acccagtcca gtatcacaac cagtgacgac
      781 cgcaacttta ccttcgagag aaaatgcact taaaatcata acaatacctt attcgtttat
      841 gcccacaact agcgcaaatc tttaacggcc acatggtcca tatcatcaaa gacctggttt
      901 tcaccaacca tgccccagat aaaggtataa gctttggttc cgacgccgga atggatcgac
      961 cagctcgggg agatcaccgc ctgctcgtta tgcatcacaa tgtgacgcgt ttcttgcggt
     1021 tgccccatca tgtggaaaac gcaggcgtca tcatccatat tgaaatagaa ataaacttcc
     1081 atccggcgct cgtgggtgtg acacggcatg gtgttccaca agttacctgg agccagctcc
     1141 gtcagcccca tactcaattg gcaggtttcc agcacatccg ggacaaaata tttgttaata
     1201 gtgcgacggt tactggtgag gttatcgcct aacgtgactg gagatacttc gtccggggtg
     1261 acttttttgg tgggatacgt cgtatgcgcg ggtgcgcaat tgtaataaaa cttcgccgga
     1321 gtgccggtat caatactggc aaagacaact tcttttgcac ctttaccaac atacagggcg
     1381 tcgcggtgac cgatttcata gcattggcca tcgacagtaa tcgtaccggc accgccaata
     1441 ttgataacac ctaactcgcg acgttcaagg aaatagctta cgccgagttg tttaccaact
     1501 tccccgccaa cggaaaccgt tttagttatc ggcataatgc cgccaacaat aattcggtca
     1561 atgtggctgt aaaccatggt gtactcatcg gcgacaaata ccttttcaac caaaaattca
     1621 ttgcgcagcc cttgggtatc cagcgttttt gcgtgcgcac tgtggatgct ctgtcttacg
     1681 tccacatcaa cctccgataa tcagtgaagc gtgaaaatag aaaacgatag tgaaacactg
     1741 tttcgttttc tctataaagg gatgatatgc ccatgggcta gggttttcaa ttaaaaataa
     1801 aacaatgttt catttttaag ttaaggatta aaaaaagtgc agtgtcgatc acgaatatgt
     1861 catgagaatg gaaaaatgag ggagcccaga aaatgtatgt cgacttgcca gccatcatta
     1921 ttcaacagat taatgacggc agatatttat cggtaaggag gaaatgcaga gctattcgtc
     1981 acgttcaatg gtcaatgcca caccctggcc cccgccgata caaagcgttg ccagtccttt
     2041 gcgggcatta cgtttcacca tttcatgaac cagagaaacc aggattcggc aaccggaagc
     2101 gcctatcggg tgaccaagtg cgatcgcacc accattgaca ttgacccgac gctcatccca
     2161 ctcaagcatc ttgccaaccg aaagcgcctg tgcagcaaac gcttcattag cctcgataag
     2221 atcgacatca gccaactgcc agcctacacg ctccaggcaa cggcgggtcg catacaccgg
     2281 cgcaattccc atcaatgccg gatctacacc aacgctggca aatgcgcgaa tgcgggccag
     2341 cacgggtaaa ttcaacgctc gtgctttggc ttcgctcatc atcattaccg cagctgcgcc
     2401 atcgtttatg gatgatgcat tacccgctgt cacagaaccg agactatcaa atgaaggatt
     2461 taaacgggct aaaccttctg cactggcgtc agtgcgtggc tgttcatcgg tatcaacaac
     2521 caacgtctga ccgttacttt gggtcattac cgggacgatc tcatctttaa atcgtccggc
     2581 gtcaatcgcc gctcgcgctt tttgttgcga gctaagtgcg taagcatcct gcaattgacg
     2641 gctgatgcca tattcgcgag ccagattttc ggcggtgaca ccaatatgat aatcattgaa
     2701 ggcatcccat aacccatcat gcacaagact gtcaaccagc tggctattgc caagctgtgc
     2761 accggtgcgg ctatcagtca gaacatgtgg tgcgcggctc atgttttcct ggccaccggc
     2821 gatgacaata tcagcctcgc cacactgtat cgcctgagta gccagatgca gtgctttaag
     2881 tccggaaccg cagacgtcat taatcgtgat tgcagaaacg ctattaggca gaccaccttt
     2941 aatagccgat tgccttgccg gattctgccc tgcccctgca gtcaacacct gaccaagaat
     3001 tacttcatcc accgcatatg caggaacgcc ggtacgttct attaacgctt tcacgaccag
     3061 actaccaagt tccacggcgg aatgacccgc taacgcaccg cgaaagcagc cgataggtgt
     3121 ccgtaacgcc ccgacaatca caacgtcttt catcacaacc tcgcaccaat taacagtgca
     3181 atagtaaatg attgttaatt acagttatct taattgttta aaaaaagtga tttttatcac
     3241 aaaggaaata tgcctgagca gtagtcagag tcataactgg c
//
LOCUS       DAPHBJ010000285         3261 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.288, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000285 DAPHBJ010000000
VERSION     DAPHBJ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.288"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            183..1499
                     /gene="shiA"
                     /locus_tag="RHZ76_004349"
     CDS             183..1499
                     /gene="shiA"
                     /locus_tag="RHZ76_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HDV2814856.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDSQRA"
     gene            1601..3055
                     /gene="amn"
                     /locus_tag="RHZ76_004350"
     CDS             1601..3055
                     /gene="amn"
                     /locus_tag="RHZ76_004350"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HDV2814857.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          751 a          803 c          791 g          916 t
ORIGIN      
        1 acaccagcaa ctcctgcgct ttcatcttcc atcgaatttt tcatgttgcc gctaatcagc
       61 cataaaatca tttgcagatg cgctctgtcg aggtagtctc ataaggttcg tttatagatc
      121 gacggcaatg tgagttacct tttccatact aattataaaa agacagtaca aacaggatca
      181 ttatggactc cacgctcatc tccactcgtc ccgatgaagg gacgctttcg ttaagtcgcg
      241 cccgacgagc tgcgttaggc agtttcgctg gtgccgtcgt cgactggtat gattttttac
      301 tctatggcat taccgccgca ctggtgttta atcgcgagtt tttcccgcaa gtaagcccgg
      361 cgatgggaac gctcgccgca tttgctacct ttggcgtcgg attccttttc cgtccgctcg
      421 gcggtgtcat tttcggtcac tttggcgacc gactgggacg taagcgcatg ttaatgctga
      481 ccgtctggat gatgggcatc gcgacagcct tgattggtat tcttccttca ttctcgacca
      541 ttgggtggtg ggcacctatt ttgctggtga cactgcgtgc cattcaggga tttgccgtcg
      601 gcggcgaatg gggaggcgcg gcgttgcttt ccgttgaaag tgcaccgaaa aataaaaaag
      661 ccttttacag tagcggtgta caagttggct acggtgtagg tttactgctt tcaaccggac
      721 tggtttcatt gatcagtatg atgacgactg acgaacagtt tttaagctgg ggctggcgca
      781 ttcctttcct gtttagcatc gtactggtac tgggggcatt gtgggtgcgt aatggcatgg
      841 aggagtccgc ggaatttgaa caacagcaac ataatcaagc ggccgcgaaa aaacgcatcc
      901 cggttatcga agcactatta cgacaccccg gtgctttcct gaagattatt gcgctacgac
      961 tgtgcgagtt gctgacgatg tacatcgtta ccgcctttgc acttaattat tcaacccaga
     1021 atatggggtt accgcgcgaa cttttcctta atattggttt gctggtcggt ggattaagct
     1081 gcctgacaat tccctgtttt gcctggcttg ccgatcgttt tggtcgccgc agggtttata
     1141 tcacaggcgc gttgatcgga acgttgagcg catttccttt ctttatggcg cttgaagcac
     1201 aatctatttt ctggatagtt ttcttctcca tcatgctggc aaacattgct catgacatgg
     1261 tggtgtgcgt gcaacaaccg atgtttaccg aaatgtttgg tgccagttat cgctatagtg
     1321 gcgctggagt cggatatcag gttgccagtg tggttggcgg tggatttaca cctttcattg
     1381 ccgctgcact catcacttac tttgccggga actggcatag cgtcgccatt tatttgctgg
     1441 ctggatgcct gatttccgca atgaccgctt tgttgatgaa agacagtcaa cgcgcttgat
     1501 agcctggcga agatcatccg atcttcgcct tacacttttg tttcacattt ctgtgacata
     1561 ctatcggatg tgcggtaatt gtatggaaca ggagacacac atgaataata agggctccgg
     1621 tctgacccca gctcaggcac tggataaact cgacgcgctg tatgagcaat ctgtagtcgc
     1681 attacgcaac gccattggca actatattac cagtggcgaa ttacctgatg aaaacgcccg
     1741 caaacaaggt ctttttgtct atccatcact gaccgtaacc tgggacggta gcacaaccaa
     1801 tccccccaaa acgcgcgcat ttggtcgttt tactcacgca ggcagctaca ccaccacgat
     1861 tactcgccct actctctttc gttcgtatct taatgaacaa cttacgttgc tgtatcagga
     1921 ttatggtgcg catatctctg tgcaaccctc gcagcatgaa atcccttatc cttatgtcat
     1981 cgatggctct gaattgacac ttgatcgctc aatgagcgct gggttaactc gctacttccc
     2041 gacaacagaa ctggcgcaaa ttggcgatga aactgcagac ggcatttatc atccaacgga
     2101 attctccccg ctatcgcatt ttgatgcgcg ccgcgtcgat ttttccctcg cacggttgcg
     2161 ccattatacc ggtacgccgg ttgaacattt tcagccgttc gtcttgttta ccaactacac
     2221 acgttatgtg gatgaattcg ttcgttgggg atgcagccag atcctcgatc ctgatagtcc
     2281 ttacattgcc ctttcttgtg ctggcgggaa ctggatcacc gccgaaaccg aagcgccaga
     2341 agaagccatt tccgaccttg catggaaaaa acaccagatg ccagcatggc atttaattac
     2401 cgccgatgct cagggtatta ctctggtgaa tattggcgtg ggaccgtcca atgctaaaac
     2461 catctgcgat catctggcag tgctacgccc ggatgtctgg ttgatgattg gtcactgtgg
     2521 cggattacgt gaaagtcagg ccattggcga ttatgtactt gcacacgctt atttacgcga
     2581 tgaccacgtt cttgatgcgg ttctgccgcc cgatattcct attccgagca ttgctgaagt
     2641 gcaacgtgcg ctttatgacg ccaccaagct ggtgagtggc aggcccggtg aggaagtcaa
     2701 acagcggcta cgtacaggta ctgtggtaac cacagatgac aggaactggg aattacgtta
     2761 ctcagcttct gcacttcgtt ttaacttaag ccgggccgta gcaattgata tggaaagtgc
     2821 aaccattgcc gcgcaaggat atcgtttccg cgtgccatac gggacactac tgtgtgtttc
     2881 agataaaccg ttgcatggcg agattaaact tcccggtcag gctaaccgtt tttatgaagg
     2941 tgctatttcc gaacacctgc aaattggcat tcgggcgatc gatttgctgc gcgcagaagg
     3001 cgaccgactg cattcgcgta aattacgaac ctttaatgag ccgccgttcc gataataagt
     3061 tctgaacctt ctcatcagaa atagatgtga aggattaagt aagacatctg gcaaatagcc
     3121 tgcaatcaac gcgggctgtt tgcttccttg ggcagatgcg agtttcatta tcgtcttaat
     3181 gatttccgca tattaaaagc atgtatgctt tcaaaatata attataaaaa atcccgccaa
     3241 caacattagt ttttataaaa c
//
LOCUS       DAPHBJ010000286         3229 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.289, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000286 DAPHBJ010000000
VERSION     DAPHBJ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.289"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(91..618)
                     /gene="mobB"
                     /locus_tag="RHZ76_004351"
     CDS             complement(91..618)
                     /gene="mobB"
                     /locus_tag="RHZ76_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDV2814858.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDRAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQDG"
     gene            complement(600..1184)
                     /gene="mobA"
                     /locus_tag="RHZ76_004352"
     CDS             complement(600..1184)
                     /gene="mobA"
                     /locus_tag="RHZ76_004352"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HDV2814859.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1254..1523
                     /locus_tag="RHZ76_004353"
     CDS             1254..1523
                     /locus_tag="RHZ76_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HDV2814860.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1600..2586
                     /gene="srkA"
                     /locus_tag="RHZ76_004354"
     CDS             1600..2586
                     /gene="srkA"
                     /locus_tag="RHZ76_004354"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HDV2814861.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEHGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAVQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2603..3229
                     /gene="dsbA"
                     /locus_tag="RHZ76_004355"
     CDS             2603..3229
                     /gene="dsbA"
                     /locus_tag="RHZ76_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HDV2814862.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          812 a          777 c          795 g          845 t
ORIGIN      
        1 cgaaaggcca tcctgacgga tggctttttt gcgttggtgc aaaaaaatgc cggatgcgac
       61 gctggcgcgt cttatccagc ctgctcttgc ttatccgtcc tgcttttgca tccactccac
      121 cacaaaatca gccagcccct caacatcatt aatatccagt aatgcgacat caagattaag
      181 cggcacatca ctggctacag caataacatg tctgtctatc actaattctt ccggtcgatg
      241 tccggctcta tcgcgaaaca gcacaatctt tgcgatctct tcatgcttaa acccttcgac
      301 cagaatcaaa tccagctttg aggtatccat tcgacttgcg agaaaatgta gatccagctc
      361 ttcttcgtct ggtgtttccg tcatcaaggc ccatcgttgc tggctggcaa cgatggtttg
      421 tgccgcgcca gccttgcgca gctcatagct atctttgcct ggcttatcaa catccatatc
      481 atggtgcgta tgcttaatca gccctggacg gatccctctg gcgcataatg ccgggatcag
      541 ttgtttcaaa agcgtagttt ttccggtacc actccacgcg gcaaaggcga gtaacggtat
      601 catcgttttt cctgccatcg ggcaagctcc tctggcgtat tcacgttaac aaatgcatct
      661 ttatgatcgc tgaaatcaac cgcatgaccg ccagccagac gcataaatgc cattacccgg
      721 cgttctcctg cttgcagata ttctagtaat aaaggctcaa tagcgcggtt taccagagca
      781 atagtcgggt gatcgcgttc accgtcatgg acccacacga caggcgcatc tttgcgctga
      841 tgattaagcc gggcggctaa atcatgggga atgtaaggcg tatcgcacgg acaaaacaaa
      901 aaccactcac cagcttcctg ctgcattact gaaagcatcc ctgccagagg gcctgggtaa
      961 tccgccagtg aatcttcaat cactttcaga ccgcctgcct gatagatttc ctgatgacga
     1021 ttagcattaa ccacgacgtg agagagctgc gtcataagcg cgtcagcgac atgttgccat
     1081 aatggtttgc cgtttaattc aagcaatcct ttatctacgc cgcccattcg tctggcttta
     1141 ccgcctgcca gcacaacgcc tgttatcgtc gtcatcagat tcaccgatat cgcctctttt
     1201 attgtgggat tgaccctgct aacgtgtctg tctcaagagt aaggagcatt actatgaaat
     1261 gtaaacgtct gaatgaagtt attgaactcc tccagccagc ctggcaaaaa gagccagacc
     1321 ttaacctgtt gcaatttttg cagaaactgg cgaaagagtc aggttttgac ggcgaactgg
     1381 cggatttgac ggatgacatt ctgatctatc acctgaaaat gcgtgattcc gcgaaagatg
     1441 cggtgatccc gggtttgcag aaagattatg aagaagactt caaaacggcg ctgttacgcg
     1501 ctcgcggcgt aattaaagag taaaagcttg taagcggcgc caccaaaatc atcgtgaaat
     1561 gatatccttc gtcattcgta atgttttccg gatgatggga tgaataacag cgcttttact
     1621 ttccagacac tacacccgga taccatcatg gacgctctgt ttgagcatgg gatccgggtg
     1681 gattccggtc ttaccccgct taacagctat gaaaaccgtg tctatcaatt tcaggacgaa
     1741 gatcgtcgac gttttgtcgt caaattttat cgccctgaac gttggacagc cgatcaaatc
     1801 ctcgaagaac atcaatttgc gttgcagctg gtaaatgatg aagttccggt cgcagcacct
     1861 gtggccttta acggtcagac tttattgaat catcagggat tttatttcgc tgtttttcca
     1921 agcgtcggtg gtcgccagtt cgaagctgat aatatcgatc agatggaagc ggttgggcgt
     1981 tatttagggc gtatgcacca gacggggcgc aaacagcttt ttatccatcg cccgaccatc
     2041 ggtttgaacg aatatctcat tgagccacgc aagctgtttg aggacgctac actgatacct
     2101 tccgggttga aagcggcatt cctgaaagcg acagatgagc tgattgccgc cgttacagca
     2161 cactggcggg aagatttcac cgttctgcgg ctacatggag actgccacgc cgggaatatt
     2221 ctctggcgcg atggtccaat gtttgttgat ctggatgatg cacgtaatgg tccagccgtt
     2281 caggatttgt ggatgttgct caatggcgat aaagccgagc agcggatgca actggaaact
     2341 attattgaag cttatgaaga atttagcgag ttcgacaccg ctgaaatcgg actgattgaa
     2401 cctttacgcg ccatgcgttt ggtttattat cttgcctggc taatgcggcg ttgggctgat
     2461 cccgcgttcc cgaaaaattt cccgtggtta accggggaag attactggct gcgacagacg
     2521 gcgactttta tagaacaggc aaaagttcta caagaacccc ctttgcaatt aacacctatg
     2581 tattaatcgg agagagtaga tcatgaaaaa gatttggctg gcgctggctg gtttagtttt
     2641 agcgtttagc gcatcggcgg cgcagtatga agatggtaaa cagtacacta ccctggaaaa
     2701 accggtagct ggcgcgccgc aagtgctgga gtttttctct ttcttctgcc cgcactgcta
     2761 tcagtttgaa gaagttctgc atatttctga taatgtgaag aaaaaactgc cggaaggcgt
     2821 gaagatgact aaataccacg tcaacttcat gggtggtgac ctgggcaaag atctgactca
     2881 ggcatgggct gtggcgatgg cgctgggcgt ggaagacaaa gtgactgttc cgctgtttga
     2941 aggcgtacag aaaacccaga ccattcgttc tgcttctgat atccgcgatg tatttatcaa
     3001 cgcaggtatt aaaggtgaag agtacgacgc ggcgtggaac agcttcgtgg tgaaatctct
     3061 ggtcgctcag caggaaaaag ctgcagctga cgtgcaattg cgtggcgttc cggcgatgtt
     3121 tgttaacggt aaatatcagc tgaatccgca gggtatggat accagcaata tggatgtttt
     3181 tgttcagcag tatgctgata cagtgaaata tctgtccgag aaaaaataa
//
LOCUS       DAPHBJ010000287         3200 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.290, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000287 DAPHBJ010000000
VERSION     DAPHBJ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.290"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            158..1840
                     /locus_tag="RHZ76_004356"
     CDS             158..1840
                     /locus_tag="RHZ76_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HDV2814863.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFQNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1892..3049
                     /locus_tag="RHZ76_004357"
     CDS             1892..3049
                     /locus_tag="RHZ76_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDV2814864.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRIPAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3015..3137)
                     /locus_tag="RHZ76_004358"
     CDS             complement(3015..3137)
                     /locus_tag="RHZ76_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814865.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
BASE COUNT          973 a          690 c          641 g          896 t
ORIGIN      
        1 taatagaaca gaaagtaatg tcaacgcttt atggactgtt atttcccttt ttttagctaa
       61 atctgctatc tctttatgtg actaacttca cttacatcca cttatttctc ttcgtaaaat
      121 tacttcggaa ttaagtacaa taagaagagg aacatttatg aagtctgcat taaagaaaag
      181 tgtcgtaagt acctcgatat ctttgatact ggcatctggt atggctgcat ttgctgctca
      241 tgcggcagat gatgtaaagc tgaaagcaac caaaacaaac gttgctttct cagactttac
      301 gccgactgaa tacagtacca aaggaaagcc aaatattatc gtactaacca tggatgatct
      361 tggttatgga caacttcctt ttgataaggg atcttttgac ccaaaaacaa tggaaaatcg
      421 tgaagttgtc gatacctaca aaatagggat agataaagcc attgaagctg cacaaaaatc
      481 aacgccgacg ctcctttcat taatggatga aggcgtacgg tttactaacg gctatgtggc
      541 acacggtgtt tccggcccct cccgtgccgc aataatgacc ggtcgagctc ccgcccgctt
      601 tggtgtctat tccaataccg atgctcagga tggtattccg ctaacagaaa ctttcttgcc
      661 tgaattattc cagaatcatg gttattacac tgcagcagta ggtaaatggc acttgtcaaa
      721 aatcagtaat gtgccggtac cggaagataa acaaacacgt gactatcatg acaacttcac
      781 cacattttct gcggaagaat ggcaacctca aaaccgtggc tttgattact ttatgggatt
      841 ccacgctgca ggaacggcat attacaactc cccttcactg ttccaaaatc gtgaacgtgt
      901 ccccgcaaaa ggttatatca gcgatcagtt aaccgatgag gcaattggcg ttgttgatcg
      961 tgccaaaaca cttgaccagc cttttatgct ttacctggct tataatgctc cgcacctgcc
     1021 aaatgataat cctgcaccgg agcaatatca gaagcaattt aataccggta gtcaaacggc
     1081 agataactac tacgcttccg tttattctgt tgatcagggt gtaaaacgca ttctcgaaca
     1141 actgaagaaa aacggacagt atgacaatac aattattctc tttacctccg ataatggtgc
     1201 ggttatcgat ggtcctctgc cgctgaacgg ggcgcaaaaa ggctataaga gtcagaccta
     1261 tcctggcggt actcacaccc caatgtttat gtggtggaaa ggaaaacttc aacccggtaa
     1321 ttatgacaag ctgatttccg caatggattt ctacccgaca gctcttgatg cagccgatat
     1381 cagcattcca aaagacctta agctggatgg cgtttccttg ctgccctggt tgcaagataa
     1441 gaaacaaggc gagccacata aaaatctgac ctggataacc tcttattctc actggtttga
     1501 tgaggaaaat attccattct gggataatta ccacaaattt gtccgtcatc agtcagacga
     1561 ttacccgcat aaccccaaca ctgaggactt aagccaattc tcttatacgg tgagaaataa
     1621 cgattattcg cttgtctata cagtagaaaa caatcagtta ggtctataca aactgacgga
     1681 tctacagcaa aaagataacc ttgccgccgc caatccgcag gtcgttaaag agatgcaagg
     1741 cgtggtaaga gagtttatcg acagcagcca gccaccgctt agcgaggtaa atcaggagaa
     1801 gtttaacaat atcaagaaag cactaagcga agcgaaataa ctaaaccttc atgcggcgga
     1861 attttccgcc gccttattga gcgagatagc gatgcacgtt acagccaagc cctccagttt
     1921 tcaatgtaat ctcaaatgtg attactgttt ttaccttgaa aaagagtcgc agtttactca
     1981 tgaaaaatgg atggatgaca gcactctgaa agagttcatc aaacaatata tcgcagcgtc
     2041 tggcaatcag gtctatttta cctggcaagg cggtgaaccc actctggctg gcctggattt
     2101 tttccgtaaa gttattcact atcaacaacg ctatgcaggc caaaaacgta tttttaatgc
     2161 attacaaacg aatggcattt tattgaataa tgaatggtgt gcctttctca aagaacatga
     2221 gtttctggtt ggtatctcga tcgatggacc ccaggagcta catgaccgtt acagacgcag
     2281 taattcaggt aatggtactt ttgcaaaagt gatagcagcc atcgagcgtc tgaaatcata
     2341 tcaagtagag tttaatacgt taaccgtcat taataacgtt aatgtccatt accctcttga
     2401 ggtttatcat tttttaaaat ctatcggcag taaacatatg caatttatcg aattgctaga
     2461 aaccgggacg ccgaatattg atttcagtgg tcatagtgag aacacattcc gtatcattga
     2521 tttttctgtg cctcccacgg cttatggcaa gtttatgtca accattttta tgcaatgggt
     2581 taaaaacgat gtgggtgaaa ttttcatccg tcagtttgaa agctttgtca gccgtttttt
     2641 ggggaatggg cataccagtt gtattttcca ggagtcctgc aaggataatc tggttgttga
     2701 aagtaatgga gacatttacg aatgcgacca ttttgtctat ccacagtaca aaattggaaa
     2761 cattaataaa tctgaactca aaacgatgaa cagtgtacaa ctgacagcgc aaaaaaaacg
     2821 gattccagcg aaatgtcagc aatgtgcata taaacctatc tgcaatggcg gttgtcctaa
     2881 gcatcgtatt actaaagtaa acaatgagac tgtttcttat ttttgcgaag gttataaaat
     2941 ccttttttca accatggtac cttatatgaa cgccatggta gagttagcta agaacagagt
     3001 accgctttac cacattatgg atgttgcaaa acaaatggag aataattaat aacgctctct
     3061 tttaactcca ttcttagcct ccttcttatg accttaacaa ggcacccgac ataaattcct
     3121 gtcgggtgtt tcgtcatcat catcttccct tgcgaaaatc ctggcttacg tggtatgcat
     3181 cagggcatta gcaatatcct
//
LOCUS       DAPHBJ010000288         3142 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.291, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000288 DAPHBJ010000000
VERSION     DAPHBJ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.291"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..635
                     /locus_tag="RHZ76_004359"
     CDS             <1..635
                     /locus_tag="RHZ76_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV2814866.1"
                     /translation="PAHAGIVIYGTRIIYPAENKEVMVQLMNQGNRSSLLQAWIDDGD
                     TSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKESIFYLNVLDIPPNSP
                     EQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNGLVIKNDSANWVTIS
                     DVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGNYISDKI"
     gene            651..3131
                     /locus_tag="RHZ76_004360"
     CDS             651..3131
                     /locus_tag="RHZ76_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV2814867.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYL
                     SQYYSDYKASGNNKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDLAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGAGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     STPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVIYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQSLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
BASE COUNT          901 a          595 c          801 g          845 t
ORIGIN      
        1 ttcctgcaca tgccggaatt gttatctacg ggacgcgcat tatttacccg gcagaaaata
       61 aagaagtgat ggtgcagttg atgaaccagg gaaaccgttc ttcgctgctg caggcgtgga
      121 ttgatgatgg cgatacgtca ttaccaccag aaaaaattca ggttcctttc atgttaacgc
      181 caccagtggc aaaaataggg gcaaattccg ggcagcaagt aaaaatcaaa attatgccga
      241 ataaactgcc cactaataaa gaaagcattt tttatctgaa tgttctggat attccaccaa
      301 atagtccaga gcaagaaggt aagaatgcac tgaagtttgc gatgcaaaac agaattaagt
      361 tgttttaccg gccagcgggt attgctccgg taaataaagc gacatttaaa aaattgctgg
      421 taaatcgcag tggcaatggt ttggtgataa aaaatgactc agctaattgg gtgacgattt
      481 cggatgtcaa agctaataat gtcaaagtca attatgaaac tattatgatt gcccccttag
      541 aaagtcagag tgttaatgtc aaaagtaata atgcaaataa ctggcatctg accattatcg
      601 atgaccatgg caactatatt agtgacaaaa tttaattgca ggagctgcct atgttgagaa
      661 tgaccccact tgcatcagca atcgtagcgt tattgctcgg cattgaagct tatgcagctg
      721 aagaaacctt tgatacccat tttatgatag gtggaatgaa agaccagcag gttgcaaata
      781 ttcgtcttga tgataatcaa cccttaccgg ggcagtatga catcgatatt tatgtcaata
      841 agcaatggcg cgggaaatat gagattattg ttaaagacaa cccgcaagaa acatgtttat
      901 caagagaagt tatcaagcgg ttaggcatta atagcgataa cttcgccagc ggtaagcaat
      961 gtttaacatt tgagcaactt gttcagggtg ggagctatac ctgggatatc ggggtttttc
     1021 gtctcgattt cagtgtcccg caggcctggg tggaagaact ggaaagtggc tatgttccac
     1081 cggaaaactg ggagcggggt attaatgcgt tttatacctc ttattatctg agtcagtatt
     1141 acagcgacta taaagcgtcg ggtaataaca agagtacata tgtacgtttt aacagcgggt
     1201 taaatttact ggggtggcaa ctgcattctg atgccagttt cagtaaaaca aataacaatc
     1261 caggggtgtg gaaaagcaat accctgtatc tggaacgtgg atttgcccaa cttctcggca
     1321 cgcttcgcgt gggtgatatg tacacatcaa gcgatatttt tgattctgtt cgcttcagag
     1381 gtgtgcggtt gtttcgtgat atgcagatgt tgcctaactc gaaacaaaat tttacgccac
     1441 gggtgcaggg gattgctcag agtaacgcgc tggtaactat tgaacagaat ggttttgtcg
     1501 tttatcagaa agaggttcct cctggcccgt tcgcgattac agatttgcag ttggccggtg
     1561 gtggagcaga tcttgatgtc agcgtgaaag aggcggacgg ctcggtaacc acctatctgg
     1621 tgccttatgc agcggtgcca aatatgctgc aacccggcgt gtcgaaatat gatttagcgg
     1681 cgggtcgtag ccatattgaa ggggcgagca aacaaagtga ttttgtccag gcgggttatc
     1741 agtatggttt taataattta ttgacgctgt atggtggctc gatggtcgcg aataattatt
     1801 acgcgtttac tttgggggct ggctggaata cacgcattgg tgccatttcc gtcgatgcca
     1861 ctaagtcgca tagtaaacaa gacaacggcg atgtgtttga cgggcaaagt tatcaaattg
     1921 cctacaacaa atttgtgagc caaacgtcga cgcgttttgg tctggcggcc tggcgttatt
     1981 cgtcgcgtga ttaccggaca tttaacgatc acgtttgggc aaacaataaa gataattatc
     2041 gccgtgatga aaacgatgtc tatgacattg ccgattatta ccagaacgat tttggccgca
     2101 aaaatagctt ttccgccaat atgagccagt cattgccaga aggttggggg tctgtgtcat
     2161 taagtacgtt atggcgagat tactgggggc gtagcggcag tagtaaggat tatcagttga
     2221 gttattccaa caacctgcga cggataagct ataccctcgc ggcaagccag gcttatgacg
     2281 agaatcatca tgaagagaaa cgttttaata tttttatatc gattcccttt gattggggtg
     2341 atgacgtttc gacgcctcgt cggcaaatat atatgtctaa ctcaacgacg tttgatgatc
     2401 aggggtttgc ctcaaataat acgggattat caggaacagt agggagtcgg gatcagttca
     2461 attatggtgt caacctgagt catcaacatc agggaaatga aacgacagct ggggcgaatt
     2521 tgacctggaa cgcgccggtt gcgacagtga atggcagtta tagtcagtcg agtacttatc
     2581 gacaggctgg agccagtgtt tcagggggca ttgtcgcctg gtcgggtggc gttaatctgg
     2641 cgaaccgtct ttccgaaacg tttgctgtga tgaatgcgcc aggaattaaa gatgcttatg
     2701 tcaatgggca aaaatatcgc acaacaaacc gtaatggagt ggtgatatac gacggaatga
     2761 caccttatcg ggaaaatcac ctgatgctgg atgtgtcgca aagcgatagc gaagcagaat
     2821 tacgtggcaa ccggaaaatt gccgcccctt atcgcggcgc ggttgtactg gttaattttg
     2881 ataccgatca gcgcaagcca tggtttataa aagcgttaag agcagatggg caatcattaa
     2941 cgtttggtta tgaagtcaat gatatccatg gtcataatat tggcgttgtc ggccagggaa
     3001 gtcagttatt tattcgcacc aatgaagtac cgccatcggt taatgtggca attgataagc
     3061 aacaaggact ttcatgcaca atcaccttcg gtaaagagat tgatgaaagt agaaattata
     3121 tttgccagta atagtcaggt gg
//
LOCUS       DAPHBJ010000289         3086 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.292, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000289 DAPHBJ010000000
VERSION     DAPHBJ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.292"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            281..460
                     /locus_tag="RHZ76_004361"
     CDS             281..460
                     /locus_tag="RHZ76_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HDV2814868.1"
                     /translation="MTTVTTPSQLKKEVESQKELLLRACLEAFNQLPNQRLQGAFTST
                     YALAAKLDQLLQQTK"
     gene            515..835
                     /locus_tag="RHZ76_004362"
     CDS             515..835
                     /locus_tag="RHZ76_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcgAII protein"
                     /protein_id="HDV2814869.1"
                     /translation="MTTQTLEQTLEDFRRQCESFARKQQPRCGLIYELYQRRLSADID
                     GYLAGVPAEYREELIAVARREFDYLTQDEIAEEIRQDRENDYCSHGIERNCCPLGCGD
                     LDDY"
     gene            complement(946..1179)
                     /locus_tag="RHZ76_004363"
     CDS             complement(946..1179)
                     /locus_tag="RHZ76_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814870.1"
                     /translation="MLQELCRVRRPGRTAYSTNEFFQLLLIRNWQQWQEQKAQLGKCQ
                     ACGKLKAEGGCGGERQSETFNCWLAVEANELNV"
     gene            complement(1472..1840)
                     /locus_tag="RHZ76_004364"
     CDS             complement(1472..1840)
                     /locus_tag="RHZ76_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StbC"
                     /protein_id="HDV2814871.1"
                     /translation="MEKQPDKFEVLMDWFLGDAKEITASQKEMTEILSALSEKLAKDT
                     ESLGETADSLKRTLVENQRSISLAISDDAKAREEFLTKFRRAQASRAETLTRQILFIT
                     AGCTIVGAAVGAAIAIILLR"
     gene            complement(1842..2558)
                     /locus_tag="RHZ76_004365"
     CDS             complement(1842..2558)
                     /locus_tag="RHZ76_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StdB protein"
                     /protein_id="HDV2814872.1"
                     /translation="MKVAVINYSGSVGKTLISSYLLAPRLTGAKFYAVETINQSASDL
                     GIENVSIFKGDDFSRLIEDIVFEDAGIIDIGASNVEAFLMAMSRFDSGANEFDKYVIP
                     VTPDNKAIDESMKTAHTLSKAGVSSDKIIFVPNRISPDSEVEDVLAPVFEFVKRTKIG
                     KISKKSVIYNSEVFEYLAYHRISFEALTAEDPEEFKARAKQTTDADERKKLARRYTYM
                     KQAIPVKANLDKAYAALMGE"
     gene            complement(2567..2704)
                     /locus_tag="RHZ76_004366"
     CDS             complement(2567..2704)
                     /locus_tag="RHZ76_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StbA"
                     /protein_id="HDV2814873.1"
                     /translation="MHNTQTPPEPEPQGSEKKESPGIIDKEFFNKISEDFDPKMFNKK
                     F"
BASE COUNT          717 a          801 c          694 g          874 t
ORIGIN      
        1 gggggacgtg cggcagctac gtgacggcgg gctgggttat ggcttagcag ggagggggat
       61 tggcctgttg ctccggtcag tcccgcagtt tccggcgatt tgcggccggt gtccggtgga
      121 gtccggcgcg gtcgccttcc atgccctgac gggcataaaa acaggtaaat aaattccgta
      181 cttgtattga cttttaaggc ataatcaccg ataattaaat catcgggagg caataccgcc
      241 acccgaaaaa ttgccggtat cggatcacta aggagtaagc atgaccactg ttaccacccc
      301 ttcccagctg aaaaaagaag tcgaatctca gaaagaatta ttgctgcgcg cctgcctcga
      361 agcgttcaac cagctgccta atcagcgcct gcaaggcgct ttcacatcga cgtatgcact
      421 ggcggcaaaa ttagaccagc tgctgcaaca aactaaatag cactgacgac ccggccaagc
      481 cgggtcttta acagaaagat cgaggacaaa cgacatgacc acacaaaccc ttgagcagac
      541 tcttgaagat tttcgtcgcc agtgtgagag ttttgcgcgc aaacagcagc cccgctgcgg
      601 cctaatttat gaactgtatc agcgccgcct gagcgcggat attgacggct atcttgctgg
      661 cgttccggcc gagtatcggg aagaactgat cgcggtagcg cggcgtgaat ttgactatct
      721 gacccaagac gagatcgcgg aagaaatccg gcaggatcgg gaaaacgatt attgcagcca
      781 cggtattgag cgtaattgtt gcccgctggg ctgcggagat ctagacgatt actgagccgc
      841 cgaaagaaaa aaacccggat tgaccgggct tttttctttc cgctgcgcgg ccgtttcagt
      901 tattactccg cgctgctttt ggctatttct gtatagcaca atacactaca cattaagctc
      961 gtttgcttcg acggctagcc agcagttaaa ggtttcgctc tgccgttcgc cgccgcaacc
     1021 tccctccgct ttcagctttc cgcaagcctg gcatttcccc agctgcgcct tttgctcctg
     1081 ccactgttgc cagtttcgga tcagcagcag ttggaaaaac tcattcgttg aataggctgt
     1141 tcgtcctgga cggcgaaccc ggcaaagctc ctgtaacatt gcttcttcct gttttattag
     1201 cgtgactgtc acgctagcgc cttcctcttt gagtcgcgcg cggcgccggg cctggcgaat
     1261 acgggcttgt tcacgctgtc ggtcattcat atttttcctc cacgtgactg tcacgttgaa
     1321 ttaaactggg cattacccct gcggggccgt tctgccgtgc tgcgcatcga gccgctacgc
     1381 gtctcgcccc ttcgggcggc ggccactaat gcctccggcc tgcggcctcc gcgcttcgct
     1441 tgaaaaaaaa ccgggacatg cccggtttac attatctcag tagaattatg gctatcgcgg
     1501 cacccacggc ggcgcccaca atagtgcagc cagctgtaat aaaaaggatc tgacgggtta
     1561 acgtctcagc tctggacgcc tgcgcgcggc ggaacttcgt cagaaattcc tcacgcgcct
     1621 tagcatcatc actaattgcc aggctaattg aacgctggtt ttctactaaa gtccgtttaa
     1681 gagagtctgc cgtctctcct aaactttcgg tgtcttttgc cagcttttcc gaaagcgcag
     1741 aaagtatctc agtcatttct ttctgacttg cggtgatttc cttcgcgtca cctaaaaacc
     1801 aatccatcag aacttcaaat ttatctggct gcttttccat tttattctcc cattaaagcc
     1861 gcatatgctt tatcgagatt agctttaaca ggaattgcct gtttcatgta ggtataacgg
     1921 cgggccagtt ttttgcgctc gtcagcatcc gttgtttgtt tagcgcgggc tttaaattct
     1981 tctggatctt cagcggtcaa tgcttcgaat gagatacggt gatacgcgag atattcgaaa
     2041 acttcactgt tataaataac agatttcttg ctgattttgc caattttcgt tcgtttgaca
     2101 aattcaaata ccggcgccag tacatcctct acttcactat ctggactaat gcggtttgga
     2161 acaaagatga ttttatcgct gcttacacct gccttactta acgtatgtgc tgttttcatg
     2221 ctttcatcaa tggccttatt atcaggcgta accgggatta catacttatc aaattcattc
     2281 gcgccactgt caaagcggga cattgccatc aggaacgctt ctacgtttga cgcaccaata
     2341 tcaataatcc cggcatcttc aaaaacaata tcttcaatca accgtgaaaa gtcatcacct
     2401 ttaaaaatgg aaacattctc aatccccaga tcggaagcag actggttgat agtttcaacc
     2461 gcatagaatt ttgctccagt cagacgcggg gctaacaggt aggatgaaat taaggtttta
     2521 ccaacgctgc cactgtaatt aattaccgct actttcataa atcacctcag aattttttgt
     2581 tgaacatctt agggtcgaaa tcttcactga ttttattgaa gaactcttta tcaataatgc
     2641 cgggactttc ttttttttca cttccttgcg gttcaggctc aggcggcgtt tgtgtattgt
     2701 gcaatacact ttcctctgca cctttctcgc tacctccatt ttgtgtattg tgcaatacac
     2761 tatcttcatt gttcggcatc cttccatgtg tattgtgcat tccatttttc cggatttgcc
     2821 gcgctctata tagggacgta tcaaagctac gaacgttcac accttccagg ccgaagcgtt
     2881 cagaaactgc tttataaatt tccgccctgg aaactcccag tgacatcatt tcttctattt
     2941 ccggcaacat caactgaagt gccgccctga tacttttggg cttcatagta ccctcattta
     3001 gaatgatgta attttgatgt atttctgatg tatttatgat gtatttttga tgtacttttg
     3061 ttgtactggc tacctcatga ccagat
//
LOCUS       DAPHBJ010000290         2944 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.293, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000290 DAPHBJ010000000
VERSION     DAPHBJ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.293"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(16..288)
                     /locus_tag="RHZ76_004367"
                     /pseudo
     CDS             complement(16..288)
                     /locus_tag="RHZ76_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            412..1815
                     /locus_tag="RHZ76_004368"
     CDS             412..1815
                     /locus_tag="RHZ76_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDV2814874.1"
                     /translation="MLEKAEAEEQLRQENDKKLLGQVLEIYDQKYVAELLRKVGKNEW
                     SRETLNRWINGKCSPKTLTSAEEELLRKMLPEAPAHHPNYAFRFIDLFAGIGGIRKGF
                     ETIGGQCVFTSEWNKEAVRTYKANWFNDAQEHTFNLDIREVTLSDKPEVPENDAYAYI
                     NEHVPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECEAQGTLFFDVARIIRAKKPA
                     IFVLENVKNLKSHDKGKTFKVIMDTLDELGYEVADAAEMGKNDPKVIDGKHFLPQHRE
                     RIVLVGFRRDLNIHQGFTLRDISRFYPEQRPSFGELLEPVVDSKYILTPKLWEYLYNY
                     AKKHAAKGNGFGFGLVNPENKESIARTLSARYHKDGSEILIDRGWDMATGETDFANEE
                     NQAHRPRRLTPRECARLMGFEKVDGRPFRIPVSDTQSYRQFGNSVVVPVFEAVAKLLE
                     PYILKAVNADSCKVERI"
     gene            complement(1849..>2944)
                     /locus_tag="RHZ76_004369"
     CDS             complement(1849..>2944)
                     /locus_tag="RHZ76_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_143212653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II site-specific deoxyribonuclease"
                     /protein_id="HDV2814875.1"
                     /translation="YIPSGIVEKLFPSINHTRELNPSVFLTAHVSSHDCPDSEARAIY
                     YNSRHFGKTRNEKRITRWGRGSPLQNPENTGALTLLAFKLDEQGGDCKEVNIWVCAST
                     DEEDVIETAIGEVIPGALISGPAGQILGGLSLQQAPVNHKYILPEDWHLRFPSGSEII
                     QYAASHYVKNSLDPDEQLLDRRRVEYDIFLLVEELHVLDIIRKGFGSVDEFIALANSV
                     SNRRKSRAGKSLELHLEHLFIEHGLRHFSTQAITEGNKKPDFLFPSAGAYHDTEFPVE
                     NLRMLAVKTTCKDRWRQILNEADKIHQVHLFTLQEGVSLAQYREMRESGVRLVVPSSL
                     HKKYPEAVRAELMTLGAFIAELTELYADIP"
BASE COUNT          782 a          708 c          716 g          738 t
ORIGIN      
        1 ggtaatgact ccaacttact gatagtgttt tatgttcaaa taatgcccga tgaccttgtc
       61 atgcaactcc accgattttg agaacgacag tgacttccgt cccagccttg ccagatgttg
      121 tctcagattc aggttatgtc gctcaatgcg ctgagtgtaa cgcttgctga taacgtgcag
      181 ctttcccttc aggcgggatt catacagcgg ccagccatcc gtcatccata ccacgacctc
      241 aaaggccgac agcaggccca gaagacgctc cagcgtggcc aacgtgcgtt acctaaaacc
      301 tgtttaaata tccagataaa aacattcaat ctgggtcaaa tgagtgatac agtttcaccc
      361 ataagaccca atggaggcaa tatgtctgaa tttgaattac tggcgcagga tctgcttgag
      421 aaagcagaag cggaagaaca actgcgacag gaaaatgata aaaagctgct cgggcaggtg
      481 ctggaaatct atgaccagaa gtacgtggct gaactgctta gaaaagttgg taaaaatgag
      541 tggagtcgcg agactcttaa tcgctggatt aatggtaagt gctcacctaa gacgctgacg
      601 tcagccgaag aggaacttct acgaaaaatg cttccggaag cgcctgcaca tcaccctaac
      661 tatgccttcc ggtttattga cctgtttgct gggattggag gtatacggaa gggcttcgaa
      721 accatcggtg gccagtgcgt ttttaccagt gaatggaata aagaggctgt gcgcacatat
      781 aaagctaact ggtttaacga tgctcaggaa cacactttca atctcgatat tcgggaagtc
      841 acgctcagtg ataaacctga agtacctgaa aacgatgcct atgcttacat taatgagcat
      901 gtgccggatc atgatgtact tctagcaggt ttcccctgtc aaccgttcag ccttgcgggc
      961 gtaagcaaga aaaactcgct cgggcgcgcg catggtttcg aatgtgaggc tcagggaaca
     1021 cttttcttcg atgtggcgcg tattatccgc gcaaaaaaac ctgccatctt tgttcttgaa
     1081 aacgttaaaa acctgaagag ccatgacaag ggtaaaacct ttaaagtcat catggatacc
     1141 ctcgacgaac tgggctatga agttgcggat gcagctgaga tgggcaaaaa cgatcctaaa
     1201 gttatcgacg gaaagcactt tttacctcag caccgagaac gtatcgtttt ggtcggtttc
     1261 cgtcgtgatc tgaacattca ccagggcttt accctgcgcg atattagtcg tttttatccg
     1321 gaacagcgtc cgtcatttgg cgaactgctg gaacccgtgg ttgacagcaa atatatactg
     1381 acgccgaaac tctgggagta tctctataac tacgccaaaa agcacgcagc taagggtaac
     1441 ggattcggtt ttggcctcgt taatcctgaa aataaagaaa gcattgcccg tacgctttct
     1501 gctcgctatc acaaagacgg gtctgaaatt ctgatagacc gtggctggga tatggccaca
     1561 ggtgaaacag acttcgcgaa cgaagaaaat caggcgcatc ggccccgtag gctgactcca
     1621 cgagagtgcg cgcgccttat gggttttgaa aaagtagatg gcaggccttt tcgcattcct
     1681 gtgtcagaca ctcagtcgta caggcagttc ggtaactccg tagtggtgcc cgtgtttgaa
     1741 gccgtagcca aactgcttga accttatatc ctgaaagcgg ttaatgccga ttcgtgcaag
     1801 gttgaacgaa tctgatcgct cctcccggta tttatgccgg gagataatct atggaatatc
     1861 tgcgtaaagc tctgtcagct cagcaataaa cgcacctagc gtcattagct cagctctcac
     1921 cgcctccggg tattttttgt gcagagatga tggcacgacc aatctgacac ccgactcccg
     1981 catctcccga tattgagcca gagaaactcc ctcctggagt gtaaacagat gcacctgatg
     2041 aattttatcg gcctcattca gtatctgacg ccagcgatcc ttacaggtag tcttgactgc
     2101 cagcatgcgc agattttcta cgggaaactc agtatcgtgg taagcccctg cggaagggaa
     2161 aaggaaatcg ggttttttat taccttctgt gatggcctgc gtcgaaaagt gtcgcaggcc
     2221 gtgctcaatg aatagatgct ccaggtgcag ttccagcgac ttcccggctc tggatttacg
     2281 gcgattgctg acagaattgg ccagcgcaat aaattcatcc acagagccaa atcctttccg
     2341 gatgatatcc agaacatgca gttcctcaac caatagaaat atgtcgtact ccacgcgccg
     2401 gcggtcaaga agttgctcat ccggatcaag ggaatttttc acataatggc tggctgcata
     2461 ctgaataatt tcacttcccg acggaaagcg caggtgccag tcttcaggta gaatatattt
     2521 atgatttact ggcgcttgct gtagagatag tccgcctaga atctgtcctg cggggccgga
     2581 tataagcgct ccgggtataa cttcaccaat agcggtctca atgacgtcct cttcatcagt
     2641 gctggcgcat acccaaatat ttacttcctt acagtccccc ccttgctcat caagcttgaa
     2701 agctaggagc gtcagagcac ctgtattttc aggattctga agtgggctgc ctctacccca
     2761 gcgggtaatc cttttttcat tccgggtttt accaaaatga cggctgttat aataaattgc
     2821 ccgggcttcg ctgtcagggc aatcatgcga tgacacatgt gcggtgagaa aaaccgaagg
     2881 gttcagttca cgggtatggt tgatagacgg aaagagtttt tcaacgatac ctgaagggat
     2941 ataa
//
LOCUS       DAPHBJ010000291         2939 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.294, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000291 DAPHBJ010000000
VERSION     DAPHBJ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.294"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(154..1554)
                     /locus_tag="RHZ76_004370"
     CDS             complement(154..1554)
                     /locus_tag="RHZ76_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HDV2814876.1"
                     /translation="MAINDAGMFTVKEINRLKILQDVIDRNIRPGQAAEMLGITPRHC
                     SRLLKRYRQYGPLGMNNQSRGRTGNRLLPASLTDQALSIIREHYRDFGPTLAREKLEE
                     VHGLVLGKETIRRLMIKAGLWIPRRQRAPKIHQPRYRRPCTGELIQIDGCDHHWFENR
                     GRPCTALVYVDDATSRLMHLLFVKSESTFTYFEATRGYIEKYGKPMILYSDKASVFRV
                     NNKHATTGPGETQFARAMRCLNITPLCAETSQAKGRVERAHLTLQDRLVKELRLKGIC
                     TIEAANAFAEEYMADYNRRFAKEPLHDFNAHRPLALDDDLDAEFTWREPRRVSKNLTV
                     QYDKMLYLIEDNEYSRRAIGKYIDVWHYPDGHKELRLNGVLLPYSTYDRLSEVDPVAI
                     VDNKRLGHVLDVARQVQRKRDNNRSQSLPCSGDEPSRRRHAPSINKSQRSLNEDDLLE
                     AMIKLQGSSEAIFGKR"
     gene            1836..>2939
                     /locus_tag="RHZ76_004371"
     CDS             1836..>2939
                     /locus_tag="RHZ76_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV2814877.1"
                     /translation="MAYSILHLSRNNQRTHLIVDDVTTLPVMFATIYGMNELSKKHLG
                     TQGNILCSLRFFYVYYYKKHKRTFDYDFYRSGYNISCFIRQLDGFFTYLLGKQHLSDE
                     TDIISNGFLHSALSRTNKSTYGNHVRNVGRFLKYLNYRYMNLAYQDMSPTEAHQINQA
                     NHRDLAARIKVFNRVEVSRNEPAHRYKSITSQQSIELTNMLIPSTPEFSDIETGELFT
                     AVVNPQNPFDSGFQQYRNYLIHRLMFNYGLRVGEVQLLMKDCVGPTLPDSRGNIRFIL
                     IVQNLPDDVVDPRKQQPSLKTEHSQRQIELTEDDFIMFTIYMEQYRSPLFEEKKIVDH
                     EFVFIKGSGRLTPLTYDAIRTFYKEKIDPAFIAL"
BASE COUNT          782 a          692 c          662 g          803 t
ORIGIN      
        1 ctgtgacacc cgaattttac ctcggtttcc tttgtatata atatcattga agttatttgc
       61 atttctcaga aatcttctct ggctttgatt gcaatagccg aaagatagcg cacacatcgc
      121 cgttggtcag ttagtacgag gtttccgcca agttcacctt ttaccaaaga tggcctcaga
      181 gcttccctga agctttatca tggcttcaag taggtcatct tcgttcagtg agcgctgaga
      241 tttgttgatg ctaggggcgt gccgtctgcg ggaaggctca tcaccagaac aaggtaacga
      301 ttgtgagcga ttattgtccc gttttctctg gacctgacgg gcaacatcca acacatggcc
      361 aagtctcttg ttatctacaa tggcgacggg atcaacctca gaaagccggt cataggtgga
      421 gtagggcagc agcacgccat ttagtcgcag ctccttatgt ccgtcaggat aatgccagac
      481 atcaatatac ttgccgatgg ctcttcgact gtactcgttg tcctcaatca gatagagcat
      541 tttatcgtat tgtacagtca gattttttga tacccggcga ggctctcgcc aggtaaattc
      601 tgcatcaagg tcatcgtcta atgccagcgg ccggtgcgcg ttaaaatcat gcaagggttc
      661 tttggcaaag cgccggttat aatccgccat gtattcttct gcaaatgcat tggccgcttc
      721 tattgtacag atccccttaa gcctgagctc cttaacgagt cggtcctgaa gtgtcaaatg
      781 agcacgttca acgcggcctt ttgcctggct ggtttcagca cacaaaggag taatattaag
      841 gcagcgcatg gccctggcaa actgggtttc gccggggccg gtggtggcgt gcttattatt
      901 gactctgaat acgctggctt tatcgctgta gaggatcatc ggtttaccgt atttctcgat
      961 atagccacgt gtagcttcaa aataggtaaa tgtggactca gatttcacaa acagcaggtg
     1021 catcaggcga ctggtggcat cgtcaacata gaccagggca gtacagggtc gaccccggtt
     1081 ttcaaaccag tggtggtcgc agccatcaat ttgtatcagc tcgccagtac aaggacgccg
     1141 gtaacgaggc tgatgaattt tcggtgcgcg ttgtctgcgg ggaatccaga gtccggcctt
     1201 tatcatgaga cgtcggatgg tttcctttcc aaggaccaga ccgtgaactt cctccagctt
     1261 ttcccgcgct agcgtggggc caaaatcacg gtagtgctcc cggattatac tgagtgcctg
     1321 atctgtcagt gacgctggca acagacggtt accagtgcgg ccacgactct ggttgttcat
     1381 gccaagcggc ccatactggc gataacgttt gagcagacgg ctgcaatgac gtggcgtgat
     1441 gcccagcatt tcggcagcct gtccaggacg gatattgcga tcgatgacat cctgaaggat
     1501 tttgaggcga ttgatttctt tcacggtgaa cattccggcg tcatttatag ccatagaaaa
     1561 ctcctcatca ggaaaaagtg agtcgttgtc tagacatagg gatctgagaa actatagcct
     1621 ggacattcct aatgagccac aaatagacat tagcattgag cctctacaga cttagtgcgc
     1681 ataagatgga ttatgttaaa tatggtgggt ttgctcttaa cttcatgagt tcctctcttt
     1741 cagtaactta ccgatgaatc agtcagttgc caaagtttag ttgtggactt acgatgactt
     1801 cataacgcaa aaaatacgag gcatccgcta agttcatggc atattccatc ttacatttat
     1861 cgaggaacaa ccagagaact catctcatag ttgatgatgt gactacccta ccggtcatgt
     1921 tcgccacaat ttatggaatg aatgaactga gcaagaaaca tctggggact caaggaaaca
     1981 ttctgtgttc gctgcgtttc ttttacgtct attactataa gaagcacaaa cgaacatttg
     2041 actacgattt ctatcggtct ggctacaata tctcctgttt tatcaggcag cttgatggct
     2101 tctttactta cctgctcggg aagcagcacc tgagtgacga gaccgacata atctctaacg
     2161 ggtttttaca ttcggcactc tccagaacca acaagtccac ctatggtaac cacgtcagaa
     2221 acgttggccg attccttaag tatctcaact atagatatat gaatctggca taccaagata
     2281 tgtcacctac tgaagctcac cagattaacc aggcgaacca tagagatctc gctgccagaa
     2341 ttaaggtctt caatagagtt gaagtatcca ggaacgaacc agctcaccga tacaaaagta
     2401 tcaccagcca gcaaagtatc gaactaacga acatgttaat cccctccact cctgaatttt
     2461 cagatatcga gaccggcgaa ctattcacgg ccgtggttaa tccccaaaac ccattcgatt
     2521 caggatttca acaataccgt aactacctta ttcaccggtt aatgtttaac tatggcttac
     2581 gtgttggtga agttcaattg ttgatgaagg attgcgtggg gccaaccctt ccagactcac
     2641 gaggtaacat tcgtttcatc ctgattgtac aaaatctacc agatgatgta gttgacccaa
     2701 ggaaacaaca accttcactc aagactgagc attcacagcg acaaatcgaa ctaaccgaag
     2761 atgacttcat tatgtttacc atttatatgg agcaatatcg gtccccatta tttgaagaga
     2821 aaaaaattgt tgaccatgag tttgttttta ttaaaggctc aggtaggtta actccattaa
     2881 cttacgatgc aattcgaaca ttttataagg aaaaaattga tccggcattc attgcccta
//
LOCUS       DAPHBJ010000292         2928 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.295, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000292 DAPHBJ010000000
VERSION     DAPHBJ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.295"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1395
                     /locus_tag="RHZ76_004372"
     CDS             <1..1395
                     /locus_tag="RHZ76_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDV2814878.1"
                     /translation="PTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRISDLAEQNKI
                     IAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKEDLIKLVEFD
                     NKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNNGGIVMMQVQ
                     KMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLDDSTKLSLPL
                     NQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVETGPIGGITSQ
                     GIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKFNGKIMGTGG
                     FIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEITFSGKIALE
                     RGLDVHYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPELKLMDERLF
                     IDAAMGFVLPEAAH"
     gene            1409..2560
                     /locus_tag="RHZ76_004373"
     CDS             1409..2560
                     /locus_tag="RHZ76_004373"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDV2814879.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(2603..>2928)
                     /locus_tag="RHZ76_004374"
     CDS             complement(2603..>2928)
                     /locus_tag="RHZ76_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2814880.1"
                     /translation="ITLEQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKWS
                     LTNTELSQAEISWRVGYENVDHFGKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQP
                     VSLAG"
BASE COUNT          775 a          678 c          757 g          718 t
ORIGIN      
        1 ccaacagggc ttggcgatcg cgccgaccgc ggtattagtc cgctggcgca agaaggtctg
       61 gtgaaatggg cattatgcgg tcactgggga caatcgccgc gtatttctga tctcgcagaa
      121 caaaataaaa ttattgctta taactatcca caaggtgtac ttacacaaac cttacgcgcc
      181 gccgcagccc accagcctgg tattattagt gatattggca tcgggacatt tgtcgatcca
      241 cgccagcaag gcggcaaact gaatgaagtc actaaagaag acctgattaa actggtcgag
      301 tttgataaca aagaatatct ctattacaaa gcgattgcgc cagatatcgc cttcattcgc
      361 gctaccacct gcgacagcga aggctacgcc acttttgaag atgaggtgat gtatctcgac
      421 gcattggtta ttgcccaggc ggtgcacaat aacggcggta ttgtgatgat gcaggtgcag
      481 aaaatggtta agaaagccac gctgcatcct aaatctgtcc gtattccggg ttatctggtg
      541 gatattgtgg tggtcgatcc ggatcaaacc caactgtatg gcggtgcacc ggttaaccgc
      601 tttatttctg gtgacttcac ccttgatgac agtaccaaac ttagcctgcc cctaaaccaa
      661 cgtaaattag ttgcgcggcg tgcattattc gaaatgcgta aaggcgcggt ggggaatgtc
      721 ggcgtcggta ttgctgacgg tattggcctg gtcgcccggg aagaaggttg tgctgatgac
      781 tttattctga cggtagaaac aggtccgatt ggcggaatta cttcacaggg tatcgccttt
      841 ggcgcgaacg tgaatacccg cgccattctg gatatgacgt cccagtttga tttttatcac
      901 ggtggtggtc tggatgtttg ttatttgagt tttgctgaag tcgaccagca cggtaacgtc
      961 ggcgtgcata aattcaatgg caaaatcatg ggtaccggtg gatttattga tatcagtgcc
     1021 acctcgaaga aaattatttt ctgcggcacg ttaactgcgg gcagtttaaa aacagaaatt
     1081 gccgatggca aattgcatat cgtccaggaa ggacgggtaa ataaatttat tcgggaacta
     1141 ccggaaatta cttttagcgg aaaaatcgcg ctcgaacggg gactggatgt tcattacatc
     1201 accgaacgtg ctgtattcac gctgaaagaa gacggcctgc atttaatcga aatcgcccct
     1261 ggcgtcgatt tacaaaaaga tattctcgac aaaatggact tcaccccagt gatttcgcca
     1321 gaactcaaac tgatggacga aagattattt atcgatgcgg cgatgggttt tgtcctgcct
     1381 gaagcggctc attaacagga gtataatgat ggatttttct ttaactgaag aacaagaact
     1441 gctgctggcc agtattcgcg aactgattac gactaacttt ccggaagagt atttccgcac
     1501 ctgcgatcaa aacgggacat atccgcgtga gtttatgcgg gcgctggcgg ataacggcat
     1561 ttctatgctt ggcgtgccgg aagagttcgg tggcatccct gcggattacg tcacccagat
     1621 gctggcgctg atggaagtgt caaaatgcgg tgctccggca tttttgatta ccaacggtca
     1681 atgtattcac agtatgcgcc gtttcggttc tgcagagcag ctacgtaaaa cggcagagag
     1741 caccctggaa acgggtgatc ccgcctatgc cctggcgttg acggaaccag gtgctggctc
     1801 agataacaac agtgccacta ccacttacac gcgtaaaaac ggcaaggtct acatcaacgg
     1861 acaaaaaacc tttattaccg gtgcgaaaga gtacccgtat atgctggtgc tggcgcgcga
     1921 tccgcaaccg aaagatccca aaaaagcctt caccctgtgg tgggtcgact ccagtaagcc
     1981 cggcattaag attaacccgc tgcataaaat cggctggcat atgctcagca cctgcgaagt
     2041 ctatctcgac aatgtggaag ttgaagagag cgacatggtg ggcgaagaag gaatgggttt
     2101 cctcaatgtg atgtacaact ttgagatgga gcgcctgatc aacgccgcgc gcagcaccgg
     2161 ctttgccgaa tgcgcctttg aagatgccgc ccgctatgcc aaccaacgta tcgcttttgg
     2221 taaacccatt ggtcataacc agatgatcca ggaaaaactg gcgctgatgg cgattaagat
     2281 cgacaacatg cgcaacatgg tgttgaaagt ggcatggcaa gccgatcagc atcagtcact
     2341 gcgcaccagc gcggcgctgg caaaactgta ctgcgcacgt accgcaatgg aagtcattga
     2401 tgatgcgatt caaatcatgg gcggtctggg ctataccgat gaggcgcgcg tctcccgctt
     2461 ctggcgtgat gtccgttgtg aacgtatcgg cggcggtaca gacgaaatta tgatttacgt
     2521 agcaggtcgg cagatcctga aagattatca gaacaaataa tctgcaggcg gcgcagcttc
     2581 ttaacaaact gcgccgccag atttatccgg caagacttac tggttgagga aattcagata
     2641 ggatttcttg ttccgcaaaa cagtttttaa attgcctgcg gtaatcgctg ggcgaacagc
     2701 cgacatggcg caaaaacagt ttgccaaagt gatcgacatt ttcataaccc acacgccagg
     2761 agatctctgc ctgtgataat tcagtattag tgagtgacca tttcgcttcc gtcatacgcc
     2821 gttgaataac atagttaata ggggaaatac gatactcttt ggtaaattca tggcagatat
     2881 aactgacgct ggcacgaaat tttttcgata actgctctaa agtgattt
//
LOCUS       DAPHBJ010000293         2899 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.296, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000293 DAPHBJ010000000
VERSION     DAPHBJ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.296"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(139..215)
                     /locus_tag="RHZ76_004375"
     tRNA            complement(139..215)
                     /locus_tag="RHZ76_004375"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(179..181),aa:Asp,seq:gtc)
     gene            complement(348..1079)
                     /gene="dnaQ"
                     /locus_tag="RHZ76_004376"
     CDS             complement(348..1079)
                     /gene="dnaQ"
                     /locus_tag="RHZ76_004376"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HDV2814881.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEINNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DDELAAHEARLDLVQKKGGSCLWRA"
     gene            1144..1611
                     /gene="rnhA"
                     /locus_tag="RHZ76_004377"
     CDS             1144..1611
                     /gene="rnhA"
                     /locus_tag="RHZ76_004377"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDV2814882.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1608..2330)
                     /locus_tag="RHZ76_004378"
     CDS             complement(1608..2330)
                     /locus_tag="RHZ76_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDV2814883.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2364..>2899
                     /gene="gloB"
                     /locus_tag="RHZ76_004379"
     CDS             2364..>2899
                     /gene="gloB"
                     /locus_tag="RHZ76_004379"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDV2814884.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSVLPDDTL
                     VCCAHEYTLSNMKFALSIL"
BASE COUNT          776 a          707 c          685 g          731 t
ORIGIN      
        1 gatatggtct accatatgtg attaacacat ttacagcata acaaaaccat agaccgaaga
       61 aatagcggga tttaaatgca aaaaaaccgc aaaattaaaa atcttgcggt tctctgaact
      121 cattttcatg agtgaatttg gcggaacgga cgggactcga acccgcgacc ccctgcgtga
      181 caggcaggta ttctaaccga ctgaactacc gctccgcgtt gtgttccgtt gggaacgagg
      241 cgaatattac ggattgcctc gaccttcgtc aacggttttt ctcatctttt gaatcgtttg
      301 ctgcaaaaat cgcccaagtc gctattttta gcgcctttta caggtattta tgcccgccag
      361 aggcagcttc cgcccttctt ctgcaccaga tcaagacggg cttcatgtgc tgcaagctcg
      421 tcatctgtcg caaaaacaac gcgtaactta cttgcctgac gtacaatgcg ctgaattgtt
      481 gcttcacctt gttgctgttg tgtctctcct tccatcgcaa aagccatcga cgtttgacca
      541 ccggtcatcg ccagataaac ttccgcaagg atctgggcat cgagtaatgc cccgtgtagc
      601 gttcgtttac tgttatttat ttcgtagcgg gcacataacg catcgaggct gttgcgctta
      661 ccaggaaaca ttttcctcgc caccgcaagg ctatcggtga ccttacagaa ggtattggtc
      721 ttcggaatat cgcgcttaag caacgaaaac tcgtagtcca taaagccgat atcgaacgct
      781 gcgttatgga tcaccaactc cgcgccgcgg atatagtcca tgaactcatc ggctacttcg
      841 gcaaacgtgg gcttatcgag caaaaattca tcggcaatac catgtacgcc aaaggcttcc
      901 ggatccacca gccgatcggg tttgagataa acatggaagt tattgcccgt caggcgacgg
      961 ttcaccactt caacggcacc aatctcaatg atcttgtggc cttcatagtg cgcaccaatc
     1021 tggttcatac cggtggtttc ggtatcgaga acgatctggc gtgtaattgc agtgctcata
     1081 gcggtcattt atgtcagact tatcgtttta cagttcgatt caattacagg aagtctacca
     1141 gagatgctta aacaggtaga aattttcacc gatggttcgt gtctgggcaa tccaggacct
     1201 gggggttacg gcgctatttt acgctatcgc ggacgcgaga aaacctttag cgcaggctac
     1261 acccgcacca ccaacaaccg tatggagttg atggccgcta ttgtcgcgct ggaggcgtta
     1321 aaagaacatt gcgaagtcat tttgagtacc gacagccagt atgtccgcca gggtatcacc
     1381 cagtggatcc ataactggaa aaaacgtggc tggaaaaccg cagacaaaaa accagtaaaa
     1441 aatgtcgatc tctggcaacg tcttgatgct gccctagggc aacatcaaat caaatgggaa
     1501 tgggttaaag gccatgccgg acacccggaa aacgaacgct gtgatgaact ggctcgtgcc
     1561 gcggcgatga atcccacact ggaagatacg ggctaccaag tcgaagttta agcttgtggt
     1621 ttacgacatt gccgggtggc tccaaccgcc tggcgaattc gtggcttgtt tttactctgt
     1681 ttcatcgggt ttagcgttaa aggaatagtc cgtttccggg caacaataag ttgtaagcaa
     1741 ccaagcgcag gaatatgcgc attcaatagt tttcctccgt gtttgttcca cgggagaacg
     1801 tggaaacggc tggcgtgcag cacttcaaaa ttcaacaaag agagccagtc cagttgccgc
     1861 atcagagtaa acatccggct gttatagggc gaggtttttc gcaataccgg cacaagtttg
     1921 cgtaatccca ttaaactaat gggattgaag ccactaatca ccagccagcc atcatcaatc
     1981 aatacccgat cggtttcacg caataaacga tgcggatcgg tgcaccacgg caatgtatgt
     2041 gccagtagac aaacatcaac ggatttatcg gcaaaaggaa gatgaagtgg atccgcctgc
     2101 acctggacgg gcattccttg cgcagaaaca ttcacttgat gagaaaccgc gcacgcttcg
     2161 caattgattt ctgcgcttaa attgccaatc ttaagcagat gaaaaccata cattttagtg
     2221 aaccacgggt tgagctggcg ctccagcgcc ttgcgataaa gctctcccca gggcaaatcg
     2281 ccccagcgat caggagccac gacagtttga gggactcttg ccggtttcat cacaaccttc
     2341 cgtttcacac taagaggtaa tctatgaatc ttaacagtat tcccgccttt gatgacaatt
     2401 acatctgggt tttgaatgat gaagcaggtc gctgcctgat tgtcgatccc ggagacgcag
     2461 agccagtatt aaacgccatt gccgccaata actggcaacc ggaggccata tttctcaccc
     2521 accatcacca cgatcacgtt ggcggcgtaa aagaactggt ggaaaagttt ccacaaattg
     2581 tggtgtatgg tccacaagag acacaagata agggaacaac acaggtagtc aaagatggcg
     2641 aaactgcctt cgttttgggg catgaattta gtgtaattgc tacgccgggt cacactttag
     2701 gacatatctg ttacttcagt aaaccttatc tattttgcgg cgacacactg ttttctggtg
     2761 ggtgtggtcg gttgtttgaa gggacagcat cacaaatgta tcaatcactt aaaaagttaa
     2821 gtgtgttacc tgacgataca ttggtatgtt gtgctcatga atatacctta tcaaatatga
     2881 agtttgcttt gagtattct
//
LOCUS       DAPHBJ010000294         2888 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.297, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000294 DAPHBJ010000000
VERSION     DAPHBJ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.297"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..475
                     /gene="agaB"
                     /locus_tag="RHZ76_004380"
     CDS             <1..475
                     /gene="agaB"
                     /locus_tag="RHZ76_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDV2814885.1"
                     /translation="SSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDDI
                     QQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDLK
                     DVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIHDVPGDQKEQIPD"
     gene            514..1317
                     /gene="agaC"
                     /locus_tag="RHZ76_004381"
     CDS             514..1317
                     /gene="agaC"
                     /locus_tag="RHZ76_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HDV2814886.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGDILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1307..2098
                     /gene="agaD"
                     /locus_tag="RHZ76_004382"
                     /pseudo
     CDS             1307..2098
                     /gene="agaD"
                     /locus_tag="RHZ76_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
     gene            2099..2854
                     /locus_tag="RHZ76_004383"
     CDS             2099..2854
                     /locus_tag="RHZ76_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HDV2814887.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPSTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATDRFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          702 a          678 c          729 g          779 t
ORIGIN      
        1 gagcagtcca aatattctct taacccgtat tgataaccgt ctggttcatg gccaggttgg
       61 cgtgacctgg acatccacca tcggtgcaaa tctgctggta gtcgtggatg atgttgtcgc
      121 taacgatgat attcaacaga aattaatggg tattaccgcg gaaacctacg gctttggcat
      181 tcgtttcttt actatcgaaa aaaccattaa cgtcatcggc aaagctgcac cgcatcagaa
      241 gatcttcctg atttgccgta cgccacaaac ggtacgtaaa ttggtagaag gtggcattga
      301 cctgaaagat gtcaacgtcg gcaatatgca tttctcggaa gggaaaaagc aaatcagcag
      361 taaagtttat gtcgatgacc aggatctcgc ggacttacgt tttattaaac aacgtggcgt
      421 gaatgttttc attcatgacg tccctggcga tcaaaaagaa caaatccctg actaaatcta
      481 aaatcgcctt aatattggtt tgaggtaata aaaatgcatg aaataaccct acttcaggga
      541 ttatccctgg cggcgttagt ttttgttctg gggattgatt tttggctgga agccttattt
      601 ttattccgcc cgataatcgt ttgtacccta actggcgcta ttctcggtga tattcagact
      661 ggcttaatta ccggtggtct gacagagttg gctttcgccg gattaacccc tgcaggtggt
      721 gttcagccgc ccaacccgat tatggcgggt ctgatgacca ccgtcattgc atggtctacg
      781 ggcgttgatg ccaaaacagc aattggtctt ggcctgccgt ttagtttgtt aatgcagtac
      841 gtcattctgt tcttctattc cgctttctca ttatttatga ccaaagccga taaatgcgcg
      901 aaagaggcgg atacggcagc gttttcccgg cttaactgga caacgatgct catcgtcgct
      961 tcagcgtatg cggtgattgc tttcctctgt acttacctgg cacagggggc gatgcaggcg
     1021 ctggtgaaag cgatgcccgc ctggctgacc cacggctttg aagtggcagg cgatattctg
     1081 cctgccgttg gttttggctt gctgctgcgc gtaatgttca aagcgcaata tatcccttac
     1141 ctgatcgccg gtttcctgtt tgtttgctac atccaggtca gcaacctgtt gccggttgcc
     1201 gtactgggcg caggctttgc ggtgtatgag tttttcaatg cgaaatcccg gcagcaagcg
     1261 caaccgcagc ccgttgccag taaaaatgaa gaagaggact acagcaatgg gatctgaaat
     1321 cagtaaaaaa gatatcaccc gtctgggctt tcgttcgtcg ctgctgcaag cgagctttaa
     1381 ctacgaaagg atgcaggcgg gcggttttac ttgggcgatg ttgccgatcc tgaaaaagat
     1441 ttataaggac gacaaaccgg gcttaagcgc ggcgatgaaa gataacctcg aatttattaa
     1501 tacccacccg aatctggtcg gattcctgat ggggttatta atttcgatgg aagaaaaagg
     1561 agaaaaccgc gacaccatta aaggcctcaa agtggcactg tttggcccaa tcgccgggat
     1621 tggcgatgcg attttctggt ttactttgtt gccgattatg gcgggaattt gctcatcatt
     1681 tgccagccag ggaaacctgc tggggccgat tctatttttc gccgtttacc tgcttatctt
     1741 tttcctgcgc gtcggctgga cccacgtcgg ttattcagtc ggcgtgaagg cgatcgataa
     1801 agtgtgagag aactcgcaga tgattgcccg ttcggcaacc atcctcggga tcacggtaat
     1861 cggcgggctg atcgcttcgt atgtgcatat taacgtggtg acatcgtttg ccatcgacag
     1921 tacccacagc gtcgcgctgc agcaggattt cttcgataaa gtcttcccga acattttacc
     1981 gatggcctac accctgctga tgtattactt cctgcgggtg aaaaaagcgc atccggtgct
     2041 gttaatcggc gtgacttttg tgctctctat tgtttgttcc gcattcggca ttttgtaaat
     2101 ggaacgaggc actgcgtctg gtggtgcctc tttacttaag gaatttcatc ctgtgcaaac
     2161 ccttcagcaa gttgaaaact atacggcgtt aagtgaacgt gccagcgaat atttattggc
     2221 cgtgatccgt agcaaaccgg atgccgtgat ttgcctggcg accggagcca cgccattact
     2281 gacgtatcat tatctggtag aaaaaatcca ccagcagcag gttgatgtca gccagctcac
     2341 cttcgtgaag ctcgacgaat gggtggatct gccattaacg atgcctagta cctgcgaaac
     2401 tttcctgcaa cagcatatcg tgcagccgct ggggctacgt gaagaccagc tcatcagctt
     2461 tcgctccgaa gagataaatg agacagagtg cgaacgggta acgaacctga ttgcgcgcaa
     2521 aggcggtctg gatttatgcg ttctcggatt ggggaaaaac ggtcatcttg ggctgaacga
     2581 accgggagaa agcctgcaac cggcctgcca tatcagtcaa cttgatgcca gaacacagca
     2641 acatgagatg ttaaaaaccg cgggtcgccc cgtgactcgt gggatcacct taggcctgaa
     2701 ggatattctc aatgcccgcg aagttttgtt actggtgact ggcgaaggaa agcaggatgc
     2761 gacagatcgt tttctcacgg ctaaagtctc taccgctatc ccggcttcat ttttatggct
     2821 gcacagtaac tttatttgtt taattaacac ctaaccagat gaaaaatttg ctcaaaatgt
     2881 ttaaatta
//
LOCUS       DAPHBJ010000295         2883 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.298, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000295 DAPHBJ010000000
VERSION     DAPHBJ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.298"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            109..741
                     /gene="comR"
                     /locus_tag="RHZ76_004384"
     CDS             109..741
                     /gene="comR"
                     /locus_tag="RHZ76_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HDV2814888.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(803..1342)
                     /locus_tag="RHZ76_004385"
     CDS             complement(803..1342)
                     /locus_tag="RHZ76_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDV2814889.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1569..2873)
                     /gene="ndh"
                     /locus_tag="RHZ76_004386"
     CDS             complement(1569..2873)
                     /gene="ndh"
                     /locus_tag="RHZ76_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HDV2814890.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQINGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT          693 a          783 c          706 g          701 t
ORIGIN      
        1 atttcagtta aatagaaagc aattattttt atgggatctg gactggtgaa cggaggtgat
       61 tatttttata ataacctttc attcacagtc accaggtaca acggcatcat ggcaactgac
      121 tcaacacaat gtgtaaaaaa aagccgtggc cgcccaaaag tgttcgacag ggatgccgcg
      181 cttgataagg ccatgaaatt gttctggcaa cacggttatg aagcgacttc tcttgcggac
      241 ctcgtcgaag cgaccggagc caaagcgccc acgctatacg cggaatttac caacaaagag
      301 gggttatttc gtgccgttct cgaccgctat atcgatcgtt ttgccgctaa gcatgaagca
      361 cagctgtttt gtgaagagaa aagcgtggag tctgcgctgg ctgactattt tgctgccatc
      421 gccaactgct ttaccagcaa agacaccccg gcaggctgct tcatgatcaa caactgcacc
      481 accctctccc cagattcagg agatatcgcc aatacgttga aatcacgcca tgcgatgcaa
      541 gagcgcactt tgcagcagtt tttatgtcaa cgacaagcgc gcggggaaat cccgacccac
      601 tgtgacgtga cacatctggc agaattcctt aattgtatta ttcaggggat gtcgatcagc
      661 gcacgcgaag gtgcatcgct ggaaaaacta atgcagattg ccggaacgac tttgcgttta
      721 tggcccgaac tagtgaaata agtcgcaaaa aaagcccctc agcctgagcg aatgaggggc
      781 caaattgcag agtacagcct tgttatactt tgttattcaa aatcagttgc ccattactat
      841 ctagcgggat ctgcgtaccc ggatcgcggt ccatgcggat tttgccctgc tgatcgccaa
      901 tcttataggt cacatcataa ccgagcattt tttctgactt gtcatacacc gttttacaac
      961 gctgttgcgt agtcgtgtaa gtatcgcttt cctggagaga gccctggatc tggttaccgg
     1021 catatccacc acccagcgcc cccacaacag tggcgacatc tttaccgcga ccaccgccaa
     1081 actgatgccc tatcacgccg ccagcaacag cgccgagcac cgacccggta atgcgatttt
     1141 catcctgcac cggtcgacga tgggtcactg tgacgttgcg acactcctga cgcggtgttt
     1201 taaccgtttc cttgattggg gttgcagaaa caacctgagc gtattgcggg ccacgttcaa
     1261 acacgttcag actggccact gccgctacgc ccagcgcagc tgcgacacca atcccgatac
     1321 ccgccaacat tgatttattc acgggaacac ctccttcttt gcagttatgc caatcatgca
     1381 agcgaaggag aaaagcagct atcagacaga agatgaaaag cgcgggaatg gctggaaata
     1441 cttaatgatt cggaggagtc tgaaggaatg cctgatgcga cgcttaacgc gtcttatcag
     1501 gcctaccgct cgtaccattt gtccagggcg gatgaggcgt ttatgccaca tccgccagtg
     1561 tacgtcgatt aatgcaactt caaacgcgga cggataacgc ggttaatact ccccaccagc
     1621 atcattaatc cggttttaaa gtaaccatgc agcgcaatct gatgcattcg gtatagcgag
     1681 atatatacaa agcgcgcaat tcgtccttca atcatcattg agccgcgcgt caggttaccc
     1741 atcaggctac caacggtgga gaagttcgac agcgatacca gtgaaccatg atctttatac
     1801 tgataatttt tcagcggctt accgtttatc tgcgccagaa tattgttcat tgcgcaggtc
     1861 gccatctggt gtgcagcctg agcacgcggc ggaacaaagc ccccttccgg acgcgggcat
     1921 gacgcgcagt cgccaatagc gtaaatgtct ggatcgcggg tggtttgcag cgtcggttcc
     1981 accaccagct ggttgatacg gttagtttca agaccaccga tatctttcag gaagtctggc
     2041 gctttgatcc cggctgccca taccatcaga tccgcctcaa tatattcgcc atctttagtg
     2101 tgcaggccgc cttcatcagc actggtgacc atggtttgcg tcagcacgcg aacgccaagt
     2161 ttcgttagct cgttgtgggc cgcagcagag atacgtggcg gtaacgcagg caaaatacgt
     2221 tctcccgctt ctaccagcgt tacgttcagg gcttcgttgg tcaggccttt gtaaccgtag
     2281 ctgtgcagtt gcttgaccgc gttgtgcaat tcagcggaga gttctacacc cgtcgcgccg
     2341 ccgccgacaa tcgcaatgtt cactttgcca ttcgcgccca ggttggcgga gtatttcagg
     2401 aacagattca gcatctcctg gtggaagcga cgcgcctggt gcgggttatc gaggaaaatg
     2461 cagttctctt tgacacctgg tgtattgaaa tcgttagagg tgctacccag cgccattacc
     2521 agtgtgtcat aggcgatttt acgttccgga accagcagtt cacctttctc atcgcgcagt
     2581 tctgcaatag tgattgtttt cgcttcacga tcaatatcaa tgacggaacc cagctggaac
     2641 tggaaaccat gattgcgcgc atgggccaga tagctcaacg catcgacgcc ttcatcaagc
     2701 gagccagtcg ccacttcgtg cagcagcggt ttccacaggt ggctgtggtt acgatcgacc
     2761 agcgtaattt tggctttttt cttgcgtccc agcttatgcc ccagctgtgt tgccatttcc
     2821 agcccaccag caccgccgcc gacaatcaca atttttttca atggcgtagt caacgtgacc
     2881 ccc
//
LOCUS       DAPHBJ010000296         2782 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.299, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000296 DAPHBJ010000000
VERSION     DAPHBJ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.299"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1112)
                     /locus_tag="RHZ76_004387"
     CDS             complement(<1..1112)
                     /locus_tag="RHZ76_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016538301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HDV2814891.1"
                     /translation="MSEYRVPALRIRQGEERQLYSLAIEGKQISKIAAISRIRRGEES
                     LVGYQRPEVRNHIREIQRYIESKNPMIPNPVIIAFDKRVRFEPLTDNGDMGHLVIPFS
                     EDKDFEKPGFIVDGQQRTAALRDAEIDSFMMPVSAFIANDTEEQREQFMLVNSTKPLP
                     KTLLYELAPHTHGRLPSDLQMRKFPSLLAQRLNFGEGPLAGRIKTATNPDGVIADNSM
                     IKMIDASLREGALYRFRDPATGLGDEGKMVKLLNNFWSAVETVFTDDWDKKPRYSRLL
                     HGVGILALGSLMDEIDQVHQDYKGEPGWTEIPSYTRFVEELNRIKPLCAWSGGVWNFG
                     TDIDGQPIVRKWNELQNLSKDISLVTDFLVMNYIKAV"
     gene            complement(1109..2359)
                     /locus_tag="RHZ76_004388"
     CDS             complement(1109..2359)
                     /locus_tag="RHZ76_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HDV2814892.1"
                     /translation="MSKLKYFFPDSQDFIDPSFDFFRETRNEHRVRQRDDQYPHEVFP
                     HPYDGMLVSKAVVDGLGGGESKYTRAQRLRYFRNGMKHFFRLPENMETMGDCGAFTYV
                     NQDVPPYRVEEVIEFYETSRFNHGVSLDHIVFGYEKPGEVFSGEVLAECRRRQDITLT
                     LAQEFLNKSQKSCFTPFGVAHGWSKTSYRQSVEALLAMGYRNITMGGMVPLKTAQILE
                     TLEEIKPLLKSDTRIHLLGIARPESFADFIKFGVTSIDSTTPLQQAFKDRKNNYHTPD
                     GPAYTAVRVPQFDANPSLSRKIKSGVIDQDIARHLEKDAMAALFEYDKGALSLEKTLD
                     AVLAYERLHSGEKEADKIRADYERTLGERPWNQCQCNICQAIGINVIIFRGAERNRRR
                     GFHNIQVLYNRLQHTLSLRSEELS"
     gene            complement(2433..2738)
                     /locus_tag="RHZ76_004389"
     CDS             complement(2433..2738)
                     /locus_tag="RHZ76_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814893.1"
                     /translation="MLMLNISVAKYIVKEFTSKQLNDLNELSQKLIEELKELPEREVK
                     KGIRRSPEEVKSFILKIMEKNPGISATHALREFRDSGNSFEEKRFRTEFMALREAKP"
BASE COUNT          748 a          703 c          595 g          736 t
ORIGIN      
        1 acagctttga tataattcat aaccaaaaaa tcagttacta atgatatatc cttggacagg
       61 ttctgcagtt cattccattt acggacgata ggctgaccat caatatccgt accgaagttc
      121 cacacgccac cactccatgc acaaagcggt ttgatgcggt ttaactcttc gacaaaacga
      181 gtataggaag ggatctcagt ccagcctggt tcacctttat agtcctgatg cacctgatcg
      241 atttcatcca tcaggctacc cagcgcaaga ataccgacgc cgtgtaatag gcgggaatag
      301 cgcggctttt tgtcccagtc atcggtaaac acggtttcca ccgccgacca gaagttattc
      361 agcagtttca ccatcttccc ttcatccccc aaccctgtcg cagggtcacg gaagcgatac
      421 aatgctccct cacgaagact agcatcaatc attttgatca tcgagttgtc agcaataacc
      481 ccatcaggat tggttgccgt tttgatgcgc cccgcaagtg gaccttcccc gaagttcagt
      541 cgttgagcca acagcgaagg aaacttacgc atttgcagat cggatggcaa acgaccgtgg
      601 gtatgcggtg ccagttcgta taacaacgtt ttgggcagag gttttgtgga gttaaccagc
      661 ataaactgct cgcgttgttc ttctgtatca ttagcgataa atgcagagac cggcatcata
      721 aacgagtcga tttccgcatc ccgcaaagca gcagtacgtt gttgaccatc cacaataaac
      781 cctggcttct caaaatcctt atcttcggag aaaggtatca ccagatgccc catgtcgcca
      841 ttatctgtaa gcggttcaaa acgcacacgc ttatcaaaag cgataatcac cgggtttggt
      901 atcatcgggt ttttgctctc aatatagcgc tgaatttcgc gaatatggtt acgtacttct
      961 ggacgctgat aaccaaccaa actctcttct ccgcgacgaa tacgcgaaat cgctgcaatt
     1021 ttgctgatct gtttaccttc tatcgcaagg ctatagagtt gcctctcctc accctgtcgg
     1081 attcgcaggg cgggaacacg atattcactc atgacagttc ctctgagcgt aatgacaatg
     1141 tgtgctgtaa acggttgtac aacacctgaa tattatggaa accgcggcga cggttacgtt
     1201 ctgctccgcg gaaaatgatg acgttgatac cgatggcctg gcaaatattg cactggcatt
     1261 ggttccaagg acgttctcct agcgtacgtt cataatccgc acggatttta tcggcctctt
     1321 tttcacccga atggaggcgt tcatatgcca ggactgcatc aagagtcttc tcaagtgaca
     1381 aagccccttt atcatattca aacagggcag ccatggcatc cttttctaga tgtcgtgcaa
     1441 tatcctgatc aatcacgcca gattttattt tgcgactcaa acttggattc gcatcaaact
     1501 gagggacacg cacagcggta taggccgggc catcaggcgt atgatagttg tttttccgat
     1561 ctttgaaggc ttgctgcagc ggtgttgtcg agtcaatact ggtcacacca aacttgataa
     1621 agtcggcgaa actctctgga cgggcaatgc caagcaagtg aatacgtgta tcacttttca
     1681 gcagcggttt aatctcttcg agagtctcaa ggatctgcgc cgttttcaac ggcaccatcc
     1741 cccccatggt gatatttctg tatcccatgg ccagcagggc ctctacagac tgacgatagg
     1801 aggttttgct ccatccatgt gcaacgccaa agggagtgaa acaggacttt tgagacttgt
     1861 ttaaaaactc ttgagccaag gtcagagtga tatcctggcg tcggcgacat tcagccaaca
     1921 cttcgccact gaatacctca ccaggttttt catagccaaa aacgatatga tcgagagaaa
     1981 cgccatggtt aaagcgggaa gtttcgtaaa actcaatcac ctcttccaca cggtatggcg
     2041 ggacgtcctg attaacatat gtgaaggctc cgcaatcacc catagtttcc atattttccg
     2101 gcagacggaa aaagtgcttc attccattac ggaagtaacg caaacgctgg gcacgagtat
     2161 atttgctctc gccgccgcca agaccatcaa ctacagcttt agaaaccagc ataccatcgt
     2221 aaggatgagg aaaaacctca tgcggatact gatcgtcacg ttgacgcacg cgatgttcgt
     2281 tacgcgtttc gcggaagaag tcgaaactgg gatcgataaa atcctgacta tcagggaaaa
     2341 aatactttag cttcgacaac ttactctcct gtgacgccac tgcaaagcct caaaaaacgg
     2401 gggattctac catgaaaccc aggcaagtag cattaaggtt tagcctctct cagggccata
     2461 aactcagtcc gaaaacgttt ttcctcaaat gaatttcccg agtcacgaaa ttcacgcaac
     2521 gcgtgtgtag cactgatacc aggatttttc tccataatct ttaaaatgaa agacttaact
     2581 tcctccggac tacgacgaat tccttttttc acttcacgct caggtaactc cttgagttct
     2641 tctattaact tttgagataa ctcatttaaa tcattgagtt gttttgacgt aaattccttc
     2701 acaatgtatt ttgccacact gatattaagc atgagcatat tgcatctcag ccactgagcc
     2761 atattttggt tggttttgaa aa
//
LOCUS       DAPHBJ010000297         2770 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.300, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000297 DAPHBJ010000000
VERSION     DAPHBJ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.300"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            219..1385
                     /gene="nhaA"
                     /locus_tag="RHZ76_004390"
     CDS             219..1385
                     /gene="nhaA"
                     /locus_tag="RHZ76_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HDV2814894.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1451..2350
                     /gene="nhaR"
                     /locus_tag="RHZ76_004391"
     CDS             1451..2350
                     /gene="nhaR"
                     /locus_tag="RHZ76_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HDV2814895.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEDRRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2388..>2770)
                     /locus_tag="RHZ76_004392"
     CDS             complement(2388..>2770)
                     /locus_tag="RHZ76_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HDV2814896.1"
                     /translation="VLSRINNENYQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQ
                     ESGTSILKNELENSEDGAGQVGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIR
                     PRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT          651 a          616 c          705 g          798 t
ORIGIN      
        1 aatccctcag aaatcctcac cttaagctat gattatcagg gcttagggtc acccgttagc
       61 gcttacagcc gtcaaaaact catctcaccg ctgatggcgc aaatccttta attgctcgta
      121 aaaaacgagt tattcctaca ctataatctg attttaacga tgattcgtgc ggggtaaaat
      181 agtaaaaacg atctattcac ctgaaagaga aataaaaagt gaaacatctg catcgattct
      241 ttagcagtga tgcctcggga ggcattattc ttatcattgc cgctatcctg gcgatgatga
      301 tggccaacag cggcgcaacc agtggatggt atcacgactt tctggagacg ccggttcagc
      361 tccgggttgg ttcactcgaa atcaacaaaa acatgctgtt atggataaat gacgcgctga
      421 tggcggtatt tttcctgtta gtcggtctgg aagttaaacg tgaactgatg caaggatcgc
      481 tagccagctt acgccaggcc gcatttccag ttatcgccgc tattggtggg atgattgtgc
      541 cggcattact atatctggcc tttaactatg ccgatccgat tacccgcgaa gggtgggcga
      601 tcccggcggc tactgacatt gcctttgcac ttggtgtgct ggcgctgttg ggaagtcgtg
      661 ttccgttagc actgaagatc tttttgatgg ctctggctat tatcgacgat cttggggcca
      721 tcattatcat cgcattgttc tacactaatg acttatcgat ggcctctctt ggcgtcgcgg
      781 ctgtagcaat tgcggtactc gcggtattga atctgtgtgg tgtacgccgc acgggcgtct
      841 atattctggt tggcgtagtg ttgtggactg cggtgttgaa atcgggggtt cacgcaaccc
      901 tggcgggggt aattgtcggc ttctttattc ctttgaaaga gaagcatggg cgttctccag
      961 cgaagcgact ggagcatgtg ttgcacccgt gggtggcgta tctgatttta ccgctgtttg
     1021 catttgctaa tgctggcgtt tcactgcaag gtgtcacgct ggatggcttg acctccattc
     1081 tgccattggg gatcatcgct ggcttgctga ttggcaaacc gctggggatt agtctgttct
     1141 gctggttggc gctgcgtttg aaactggcgc atctgcctga gggaacgact tatcagcaaa
     1201 ttatggcggt ggggatcctg tgcggtatcg gttttactat gtctatcttt attgccagcc
     1261 tggcctttgg tagcgtagat ccagaactga ttaactgggc gaaactcggt atcctggtcg
     1321 gttctatctc ttcggcggta attggataca gctggttacg cgttcgtttg cgtccatcag
     1381 tgtgacagga cggtttaccg gggagccata aacggctccc ttttcattgt tatcagggag
     1441 agaaatgagc atgtctcata tcaattacaa ccacttgtat tacttctggc atgtttataa
     1501 agaaggttct gtggttggcg cagcggaggc gctttattta acaccacaaa ccattaccgg
     1561 acagattcgg gcgctggaag agcgcctgca aggcaaatta tttaagcgca agggacgtgg
     1621 tctcgaaccc agcgagctgg gagagctggt ctatcgctat gccgataaaa tgttcacctt
     1681 aagccaggaa atgctggata tcgtgaacta tcgcaaagaa tccaatttat tgtttgacgt
     1741 tggcgtggct gatgcacttt ccaaacgcct ggtcagtagc gtgctgaatg ctgcagtagt
     1801 agaaggcgaa ccgattcatc ttcgctgctt cgaatccacc cacgaaatgc tgctggagca
     1861 attaagccag cataaactgg atatgatcat ttccgactgt ccgatagatt ctacgcagca
     1921 ggaaggcctg ttctccgtga gaattggcga atgtggcgtg agcttctggt gtacaaatcc
     1981 accaccggaa aaaccgttcc cggcttgtct ggaagaccgg cggcttttga ttcctggacg
     2041 acgttcaatg ttagggcgta aattgcttaa ctggtttaac tcccagggat taaacgtaga
     2101 aatcctcggc gagtttgatg atgccgcgtt gatgaaagct tttggtgcga tgcacaatgc
     2161 aatcttcgtt gccccaacgc tttatgcata tgacttttat gccgataaaa ctgtcgtaga
     2221 aattggtcgc gtcgagaatg tgatggaaga gtatcatgct atttttgctg agcggatgat
     2281 tcagcacccg gcggtacagc gaatctgcaa tacggattat tctgcgcttt ttagtccagc
     2341 agcacgttaa tcggcagctc ccccaaagtt aaggtggggg agatagatta gttgtacatt
     2401 accacgattt tgactcggct cattatttgc ccgcttgaga cattgtttcc atatgtacgc
     2461 gggcgaataa acagaggaat ctgattactt ccttcatggg gatgctgaaa agagtagtaa
     2521 ttgcttttac catactggat ttcattcgta ccatcattgg agagttgaaa gcctacttga
     2581 cccgcaccgt cctcactgtt ttctaactca ttttttaata tgctggttcc tgattcctgt
     2641 atcgtcactg gttcaaagta atattcaaca tcactgggtt caaaaactaa ttcaccttgc
     2701 tcgccctgga tgcagtcaaa tcttatatga tgaaactgat aattttcatt attgatacga
     2761 gacagtacaa
//
LOCUS       DAPHBJ010000298         2730 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.301, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000298 DAPHBJ010000000
VERSION     DAPHBJ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.301"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..1509
                     /gene="espL1"
                     /locus_tag="RHZ76_004393"
                     /pseudo
     CDS             1..1509
                     /gene="espL1"
                     /locus_tag="RHZ76_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1680..1787
                     /locus_tag="RHZ76_004394"
     CDS             1680..1787
                     /locus_tag="RHZ76_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814897.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1840..2598)
                     /locus_tag="RHZ76_004395"
     CDS             complement(1840..2598)
                     /locus_tag="RHZ76_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HDV2814898.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          819 a          608 c          561 g          742 t
ORIGIN      
        1 agatcgtaac cattgtatga aactgtctaa aaaaatccat agagaaaaaa actatacacc
       61 caacagtaaa tctcaatggt agtgcatgtt ttttgcaatc tccttctgac gctatttttt
      121 gtcgccatct gtcattgcaa tacgcccttg attcattgag aaatggaaaa ggcaaagtca
      181 acctgattaa acattactcc tccgttgaat ccatacagca gcatgtcccc ttagtccggg
      241 acgcggagtt cagagcatta cttcgccatc ctcctgcagg gagtcgcgtt atcgcgagta
      301 aggattttgg cttcgcttta gatattttct tttgtcgaat gatggcaaac aatgtcagtc
      361 atatgtccgc gattttatat atagacaatc atactttgtc agtaaggcta cgaataaagc
      421 agtcagtgta tggacaatta aattatgttg tgtccgttta cgacccgaac gataccaacg
      481 ttgccgtcag agacacccac aggacagcac ggggctttct ttcgcttgat aagttcatca
      541 gttcaggtcc cgatgctcag acctgggctg atagatatgt tcgcaactgt gcaattgcta
      601 ttctgcccct attacctgtg ggagttccag gggctatttt cgcgggtatt gcatcacgaa
      661 tgccatttgc ccctatacat ccatcggcaa tgttgttaat aatggctaca ggccagactc
      721 aacagcttat tacattattc aaacagttgc ccatactccc tgaaaaagaa atcattgaaa
      781 taataaccgc gcagaatagc gttggtacac ctgctttatt tttggctatg atgaacggac
      841 atactgacaa cgtgaaaata tttatgcaag aaattcagtc actggtagat aatcacatca
      901 ttcatgaaga taatctggtt aaattactgc aaactaaaag tgctaacgaa acacctggac
      961 tttatatctc catgttgtat ggattcgatg aaataatcga tatctttctg aatgcattaa
     1021 ccactccaat agcacaagag cttttaaaca aaaaactggt gatgagtatt ttagccatga
     1081 aaatacatga tggtgagcca ggattatacg ccgcaatgga aaataatcac cctttgtgtg
     1141 tcacacggtt cctctctaaa attaatggca tcgcctttaa atacaagttg agcaaagcta
     1201 acatcatgga tttattaaaa ggagctacag cacagggaac ccctgcatta tacatcgcca
     1261 tgagcaaggg taatgaagac gtcgtgttat cttatatatc gacgctgggt gcttttgcaa
     1321 aaaaaacatt cttttagtca acatcagtta tttacactat tgaccgctaa aaatcatgac
     1381 aacatgtcag ctgttcatat agccattcat cataagcatt ataaaactgt agaaacatat
     1441 tatgctgcta ttaatgcaat cagccaaagc ctgagtttta gtgctgatga aataaagacg
     1501 tatttataac cagctattta cgatatatat cgatacataa ttttattttt tcataaaacg
     1561 cagaaacagt gagcaggtgt acaacgccac ccgacggctg taataacaaa aaatcccttg
     1621 atgcctgtcc cttttgttac actccgttat cacgcacaag agatatgcag gacactggta
     1681 tgccgactaa acgctttgat aaaaaacact ggaagatggt ggtggtgcta ctggcaatct
     1741 gtggcgctat gttgttgctc cgttgggcag caatgatttg gggctgagca gtgacaaacg
     1801 aagacagcca gaccgcataa cacggtccgg cagataattt tacatgctgt aacccagcga
     1861 taaagttgtc cgacgatcgg tatgttccgg tgcagactct ggaggttcag agttccatgt
     1921 gacgttgtaa gccaccttca gtccaaaatg ttcattaata gcaacattta atgcgctttc
     1981 ggagttcagc gttgtgtctt ccgcgccaaa gaccgaaaca ccctgcgtaa atttagcgtt
     2041 gtcagtcaac tgccatgcat aagccccgga ggcatagccc agcggctggg tttcactggc
     2101 attgtcggta tatttgtcgt aacgcacacc tggaccgaat tcaaagcgga aactgtgtac
     2161 cgggccattc aaaaactgac gaccataacc cgcggtcaac acatcgcgct ctcgatagcc
     2221 gttataacgg tctgtcagcc agcttgcctg accaaataaa tagtcataat cagttaaatt
     2281 aaaacggcta cgcccgcccg ccgcatattt ttctgaagaa cgctcatcat tagaagaagt
     2341 attactggcg ttcccccaca gcgaccaggc agtggtttgt ccataccagg tcatggtggt
     2401 gtcagccgta agtgaggagc ttttcgtatt gcctgattgt gcaagatatc ctgcgttcag
     2461 attaccttcg aaaggttttt tagcgctggc aggatcgtcc atgacagtaa aaacggaatc
     2521 atcggcagct gcattcagtg acgcaaacat gccccccgcc aacataacga tggcaggaac
     2581 tgtcttcaaa agcttcattt atcaagagtc cgtacaacaa aaaaagagac catcgcggtc
     2641 ccggaaactt tcttaaggat caaagattag cgtccctgga aaggtaacga attataaaaa
     2701 ggcgcgaata acttagcaat gtattcttat
//
LOCUS       DAPHBJ010000299         2716 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.302, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000299 DAPHBJ010000000
VERSION     DAPHBJ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.302"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(60..245)
                     /gene="rzoD"
                     /locus_tag="RHZ76_004396"
     CDS             complement(60..245)
                     /gene="rzoD"
                     /locus_tag="RHZ76_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HDV2814899.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSGNDW"
     gene            complement(462..680)
                     /gene="rrrD"
                     /locus_tag="RHZ76_004397"
                     /pseudo
     CDS             complement(462..680)
                     /gene="rrrD"
                     /locus_tag="RHZ76_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
     gene            668..832
                     /locus_tag="RHZ76_004398"
     CDS             668..832
                     /locus_tag="RHZ76_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 family protein"
                     /protein_id="HDV2814900.1"
                     /translation="MFPVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEEINNKRG
                     AVCTKHLLLS"
     gene            805..996
                     /locus_tag="RHZ76_004399"
     CDS             805..996
                     /locus_tag="RHZ76_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HDV2814901.1"
                     /translation="MHKASPVELRTCIEMAHSLAQIGVRFVPIPVETDEEFHTLAASL
                     SQKLEMMVAKAEADERDQV"
     gene            1073..1288
                     /locus_tag="RHZ76_004400"
     CDS             1073..1288
                     /locus_tag="RHZ76_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDV2814902.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            1387..1608
                     /locus_tag="RHZ76_004401"
     CDS             1387..1608
                     /locus_tag="RHZ76_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HDV2814903.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAVSATHCCECGDPIDE
                     LRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            1605..1856
                     /locus_tag="RHZ76_004402"
     CDS             1605..1856
                     /locus_tag="RHZ76_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HDV2814904.1"
                     /translation="MSVIHSQALREAAEQAMPDNWGFDADLFHELVTPSIVLTLLDER
                     ERNQQYIKRRDQENEDIALTVGKLRVELETAKIKTQRAA"
     gene            2155..>2716
                     /locus_tag="RHZ76_004403"
     CDS             2155..>2716
                     /locus_tag="RHZ76_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814905.1"
                     /translation="MKKSILILGLTLIVSSQIPSAMAKNESKLWVVVDRTERHTCPSS
                     KCGVAGKLFFREGVDFLEKKGEWVRITEPYSASCVGGESEYIKEGNKSCTRKNGIVNG
                     KFSEWVKFSDLSSERPSDPAENASGDDTLIKGSDDYRIYKKEFSSAARKLINEGVCTE
                     SDFKEIGGWMASSNKGENIYFTYCGGM"
BASE COUNT          798 a          582 c          684 g          652 t
ORIGIN      
        1 ttactttaaa cactgctcgg ttatgtattg ctgagcacct tcaagttggg cctgcatcat
       61 taccagtcgt tcccggaggg tgaaataatc ccgttcagcg gtgtctgcca gtcgggggga
      121 ggctgcatta tccacgccgg aggcggtggt ggcttcacgc actgactgac agactgcttt
      181 gatgtgcaac cgacgacgac cagcggcaac atcatcacgc agagcatcat tttcagcttt
      241 cgcatcagct aactccttcg tgtattttgc atcgagcgca gcaacatcac gctgacgcat
      301 ctgcatgtca gtaattgccg cgttcgccag cttcagttct ctggcatttt tgtcgcgctg
      361 ggctttgtag gtaatggcgt tatcacggta atgattaaca gcccatgaca ggcagacgat
      421 gatgcagata accagagcgg agataatcgc ggttaccctg ctcattgttg cccccacaaa
      481 cagacctcac gctcaatctc acgacgagtc atcaggcctt tccattgctt accgccagcg
      541 tatgcccagc gacgtagctg gtcacatgcg cccttgatat cgccctggtt tattttgcga
      601 agaagagtag atgttctgaa attgcctgcg cccacgttat agacgaacga gtaaagagcg
      661 ccgcgcgttg tttccggtag gtgcgatcac tttcgtctac tccgttacaa agcgaggctg
      721 ggtatttccc ggcctttctg ttatcagaaa tcccctgaaa gcacagcggc tggctgagga
      781 gataaataat aaacgggggg ctgtatgcac aaagcatctc ctgttgagtt aagaacgtgt
      841 atcgagatgg cacatagcct cgctcaaatt ggagtcaggt ttgtgccaat accagtagaa
      901 acagacgaag aatttcatac gttagccgca tccctttcac aaaagctgga aatgatggtg
      961 gcgaaagcag aagcagatga gagagaccag gtatgacaac cactgaatgc atttttctgg
     1021 cagcgggctt catattctgt gtgcttatgc ttgccgacat ggggcttgtt caatgacacc
     1081 tcagcaagaa aacgcccttc gcagcattgc ccgtcaggct aattctgaaa tcaaaaaagc
     1141 cagacagcag tttccggata aaaacgtcga tgacatttgc cgtagcgtac tgaagaagca
     1201 ccgcgaaacg gtaacgctga tgggattcac accgactcat ttaagcctgg caatcggcat
     1261 gttaaacggc gtctttaagg aacgatgaac atgaaaagca aaattatcag ggagctacag
     1321 gctccttttt tattgctcgc atttaccccc aagcgtatta accaacaatt cagggattaa
     1381 tggaagatgg cagacatcat tgattcagca tcagaaattg aagaattaca gcgcaacaca
     1441 gcaataaaaa tgcgccgcct gaaccaccag gctgtatctg ccactcattg ttgtgagtgt
     1501 ggcgatccca tagatgagct aagacgcctg gccgttcagg gttgtcggac ttgtgcaagt
     1561 tgccaggaag atctggagct tatcagtaaa cagagaggtt cgaagtgagc gtaattcact
     1621 ctcaggcact gcgtgaagcg gcagagcagg caatgcctga caactgggga tttgacgcgg
     1681 accttttcca tgagctggta acaccatcga ttgtgctgac actgctggat gaacgggaaa
     1741 gaaaccagca atacatcaaa cgccgcgacc aggagaacga ggatattgcg ctaacggtgg
     1801 ggaaactgcg tgttgagctg gagacagcaa aaatcaaaac tcaacgagca gcgtgagtag
     1861 aaggtgttat ctcggatgga agtaagcgta ttgctgaact ggaggcctgg gttgaataca
     1921 caagagctgc atacgtaaga gcaaaagaca agggagattt gatcagagtt attacccgcc
     1981 aaccaacggg attttacgct tacgtaccat gtaattagga atccttgaag tggcagccta
     2041 actgcggata cactgaaatg gcgatttggt aacatgtttc gcacaaggct gttactacac
     2101 ttagagataa tcagccatga ttaaacgctt tgtaaaaagt aaaaggaaat tacaatgaaa
     2161 aaatcaatac taattttagg gcttacgtta attgtctcat ctcaaatacc atcggcaatg
     2221 gcaaaaaatg aatcaaaact atgggttgtt gttgatcgaa cggaaagaca tacctgccct
     2281 tcaagtaaat gtggagtggc tgggaaacta tttttcaggg aaggcgtaga ttttctagaa
     2341 aaaaaaggtg aatgggttcg tataactgag ccatattcag cctcatgtgt tggaggggaa
     2401 agcgaatata ttaaagaagg taataaatcc tgcacaagaa aaaatggaat cgttaatggc
     2461 aagttttcag aatgggttaa atttagtgat cttagcagtg aaaggccatc agatcctgct
     2521 gaaaatgcga gcggagatga tactttaatc aaaggatctg atgactaccg tatatacaaa
     2581 aaagagtttt cttcggcagc taggaagtta ataaatgaag gggtctgcac ggaaagcgat
     2641 tttaaggaaa tcggagggtg gatggcatca agcaataagg gtgaaaacat ctatttcaca
     2701 tattgcggag gaatga
//
LOCUS       DAPHBJ010000300         2673 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.303, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000300 DAPHBJ010000000
VERSION     DAPHBJ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.303"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..227)
                     /locus_tag="RHZ76_004404"
     CDS             complement(<1..227)
                     /locus_tag="RHZ76_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HDV2814906.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTTGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARIMGVG"
     gene            295..1232
                     /locus_tag="RHZ76_004405"
                     /pseudo
     CDS             295..1232
                     /locus_tag="RHZ76_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491235.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1392..2196
                     /locus_tag="RHZ76_004406"
     CDS             join(1392..1742,1744..2196)
                     /locus_tag="RHZ76_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611700.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HDV2814907.1"
                     /translation="MAGNKWQISDELWEKMAPLLPEHKTHHPLGTHRRRVNNRAAMNA
                     ILFVLRTGCQWNALNATGICSSSSAHRRFQEWRDAGVFERFWQNGLLACEQLDAIDWS
                     WLSMDGCMTKSPLAGQKTGRNPTDRGKQGVKRSLMTDANGLPLSLVVAGANTHDIKLV
                     ADTLDTLQTGRPGRRLHLCMDKGYEAEWLEACLKNRRYEPHIQSRKEESEAIKSTDFK
                     AHRWVVERTHSWMNRFRRVLTLWEKKVENYVAMLYLTCGVIVWNKILLG"
     gene            2425..>2673
                     /locus_tag="RHZ76_004407"
     CDS             2425..>2673
                     /locus_tag="RHZ76_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814908.1"
                     /translation="MRIVYFVNAAWYFELHWLDRSEAAISKGYEVHLVSNFADDAIKN
                     NLEKKGIKCWDIELNRFSKNVFKNISIFTAFRKICKQIK"
BASE COUNT          714 a          616 c          727 g          616 t
ORIGIN      
        1 ccaacgccca taatgcgggc ggttgcccgg catccaacgc cattcatggc catatcaatg
       61 attttctggt gcgtaccggg ctgagaggcg gtgtaagtga actgtagttg ccatgtttta
      121 cggcagtgag agcagagata gcgctgatgt ccggtggtgc ttttgccgtt acgcaccacg
      181 ccttcagtag cggagcagga aggacatctg atggaaatgg aagccacgca agcaccttaa
      241 aatcaccatc atacactaaa tcagtaagtt ggcagcatta cccatcgtgt caccatggca
      301 aagcaaaagt ttaaaatcac caactggtca gcttacaaca aggcactcag acagcgtggt
      361 tcgctgacga tctggcttga tgaatctgct attgctgcat ggactgactg cgcaaagcct
      421 gaaggtcgtg gtcggcctct ccactacacc gatatggcga tcaccacggt tctgatgatg
      481 aagcgcgtgt ttaatctgcc actccgggca ttacagggct tcgttgactc gatttttacg
      541 ctgatggctc tgccgctgcg ttgcccggac tactctctgg tcagcaaaca tgcaaagagc
      601 gttaacatca gcataaaaac gccaacccgg ggtgaaatct cgcatctggt tatcgatgct
      661 actggcctga aggtcttcgg ggaaggcgaa tggaaagtca ggcaacatgg ggctgacagg
      721 cgcagagtat ggcgcaaact tcatctggct gcagacggtg tgacgcatga gataatctgt
      781 gccgatttat cgctcagcgg tacgacagat gcgcaggcac tgccggggct gattaaccag
      841 acccaccgaa aaataagaga agcttcagca gacggcgctt atgacacgcg caactgtcat
      901 gatgcgttgc tgaggaaaaa aatcaggcca cttatccctc cacgaaacgg ggcgcaatac
      961 tggccgggca gataccatga acgtaaccat gcggtggcta atcagcatct gagcggtggc
     1021 aatgatgtat gaaaaaagaa agtaggctac caccgacgtt ccgtggcaga aacggcgatg
     1081 ttccgtatca aaaccttgct gggcggacac ttgagcctgc gagactacga tgcgcaggtg
     1141 gggacgcgca ggtgggcgaa gcgatggcaa tggttaaagc gctaaaccgg atgacgttgt
     1201 tgggtatgcc gcatagtgtc cggatcgcat aacgggtgta ctgctggccc cctcttcggc
     1261 tgactctgat ttattcaaca aagcctgtca ggagctgact gccatgatgc ttgttttttt
     1321 attcatctta ttaagaacct atcccaaaag gaattgtcag ttaataagta atgacaattt
     1381 ctgagggaag tatggcaggt aacaagtggc agatcagcga tgaactctgg gaaaaaatgg
     1441 ctccgttgct cccggaacac aaaactcacc atccgctggg tacgcatcgc aggcgggtta
     1501 ataatcgtgc cgcaatgaac gccattctct ttgtgctcag aacgggttgc cagtggaatg
     1561 ccctgaatgc caccgggata tgctcgtcaa gctctgctca ccgccgcttc caggagtggc
     1621 gggatgcagg agtttttgaa cggttctggc aaaacgggtt gctggcctgt gagcagttgg
     1681 atgccatcga ctggtcatgg ctgtcgatgg atggctgtat gacgaagtca ccgctggctg
     1741 gctcaaaaaa caggacggaa ccccacagac cgagggaaac agggcgtaaa acgtagcctg
     1801 atgaccgatg cgaacgggct tcccctctca ttggttgtcg caggtgcaaa tacccacgac
     1861 ataaagctgg ttgctgatac tctcgatacc cttcagacgg gcaggcctgg cagaaggctc
     1921 cacctctgta tggacaaagg ctatgaagcc gaatggctgg aggcctgtct gaaaaaccgc
     1981 cgttacgaac ctcatatcca gtcaaggaaa gaggagtcgg aagccatcaa aagcacggat
     2041 tttaaagctc accgctgggt cgtggagaga acgcacagtt ggatgaatcg cttccgtcgg
     2101 gtgttgactc tctgggagaa gaaagtcgaa aattatgtgg caatgctgta tcttacctgc
     2161 ggcgtcattg tctggaataa aatcctattg ggataggctc taagattatt acactcaaaa
     2221 tacaacccgt tgtcttgagt aatactacgc tgcaatggga gtgttaggct tagggtagtt
     2281 gcagcggtca gtagcctggc cgtcaatact cctgacaaaa aacaagagtt tcgccctttg
     2341 gcagccagag ccaagggcgg tagcgtgcct acattacatg tagtaagccg tgattgacca
     2401 tctcgcgagt tgttgaggaa tggtatgagg atagtgtatt ttgtcaatgc agcgtggtac
     2461 ttcgaactgc attggttgga ccggtcagaa gcggcaatta gcaaagggta tgaagttcat
     2521 cttgtaagta attttgctga tgacgccatc aaaaataacc tggaaaaaaa aggtataaaa
     2581 tgttgggata ttgagcttaa cagattttct aaaaatgtgt ttaaaaacat ttcaatattc
     2641 actgcattta gaaagatctg taagcaaatt aaa
//
LOCUS       DAPHBJ010000301         2672 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.304, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000301 DAPHBJ010000000
VERSION     DAPHBJ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.304"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1390
                     /locus_tag="RHZ76_004408"
     CDS             <1..1390
                     /locus_tag="RHZ76_004408"
                     /inference="COORDINATES: protein motif:HMM:NF015371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDV2814909.1"
                     /translation="SQAATPKAVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSTSE
                     TLAATPKAVKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGGAGW
                     FKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQRT
                     LTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPEGL
                     TDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQAFDKSAYPKLAAAYPSGVI
                     PDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNT
                     GAHTHSLSGSTNAAGNHSHRDGRRFNPSVFKDTYQYGYTSSGQNTWGVQGSVGMSTGW
                     LANTSTDGNHSHSLSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTV
                     KNIAFNYIVRLA"
     gene            1390..1965
                     /locus_tag="RHZ76_004409"
     CDS             1390..1965
                     /locus_tag="RHZ76_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDV2814910.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDKASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAQDIEWPALP"
     gene            complement(2063..2653)
                     /locus_tag="RHZ76_004410"
     CDS             complement(2063..2653)
                     /locus_tag="RHZ76_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV2814911.1"
                     /translation="MSRIFAYCRISTLDQTIENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTEIGIRVHCLALGG
                     IDLTSPTGKMMMHVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
BASE COUNT          725 a          605 c          653 g          689 t
ORIGIN      
        1 gtcacaggcg gcaacgccaa aagccgttaa ggccgcgtat gacctggcta acgggaaata
       61 caccgcacag gacgctacga cagcacaaaa aggaattgtc cagctcagta gtgcaaccaa
      121 cagcacatct gaaacgcttg ctgcgacacc gaaagcagtg aaagcagcta atgacaatgc
      181 gaatggtcgg gtaccttctg cccgtaaggt gaatggtaag gcgctttcag cggatataac
      241 actgacgccg aaagatattg gtacgcttaa ctcaacaaca atgtcattca gcggtggtgc
      301 tggttggttc aaattagcaa cggtaaccat gccacaggcg agttctgttg tttcaattac
      361 gttgattggt ggcgctggat ttaacgtggg gtcacctcaa caggcaggta tatctgaact
      421 tgttttacgt gcaggtaatg gtaatccgaa ggggattact ggtgctttat ggcagcgcac
      481 attgacaggg tttacaaatt ttgcctgggt taatacatct ggtgatactt acgatattta
      541 cgttgcaatc ggaaattatg cgactggtgt aaatattcaa tgggattata ccagtaatgc
      601 cagcgtgacg attcatacgt caccagcata ttctgctaat aagccggaag ggttaacgga
      661 cggtacagtt tattcactct atacgccatc agagcagttt tatccgcctg gcgcaccaat
      721 cccgtggcca tcagataccg ttccgtctgg ctatgccctg atgcaggggc aggcttttga
      781 caaatctgca tacccgaaac ttgcagcggc ttatccgtca ggcgtgatcc ctgatatgcg
      841 tggctggacg attaagggca aacctgccag tggtcgggcc gtattgtctc aggaacagga
      901 cggcattaaa tcgcataccc acagcgccag cgcatccagt acggatttgg ggacgaaaac
      961 cacatcgtcg tttgattacg gcactaaatc cacgaataac actggtgcgc atacccatag
     1021 tttaagtggc agcacgaatg cagctggtaa tcacagccat agagatggcc gtcgatttaa
     1081 ccccagtgtt tttaaagata cttatcaata tggttataca agctcaggtc aaaatacctg
     1141 gggtgtacaa ggctcagtag gtatgtctac ggggtggtta gcgaatacca gtacagatgg
     1201 taatcatagc cattcactgt ccggcacagc agcatctgca ggtgcacacg cgcatactgt
     1261 cggtattggt gctcatacgc actccgttgc gattggttca catggacaca ccatcaccgt
     1321 taacgctgct ggtaacgcgg aaaacaccgt caaaaacatc gcatttaact atattgtgag
     1381 gcttgcataa tggcattcag aatgagtgaa caaccacgga ccataaaaat ttataatctg
     1441 ctggccggaa ctaatgaatt tattggtgaa ggtgacgcat atattccgcc tcatacaggt
     1501 ctgccagcaa acagtaccga tattgcacca ccagatattc ctgctggctt tgtggctgtt
     1561 ttcaacagtg ataaggcatc gtggcatctc gttgaagacc atcggggtaa aacggtttat
     1621 gacgtagcgt caggggacgc gttatttatt tctgaactcg gtccgttacc ggaaaatgtt
     1681 acctggttat cgccggaagg ggagtttcag aagtggaacg gcacagcctg ggtgaaagat
     1741 gcagaagcag aaaaactgtt ccggatccga gaggcggaag aaacaaaaaa cagcctgatg
     1801 caggtagcca gtgagcatat tgcgccactt caggatgctg tagatctgga aatcgcaacg
     1861 gaggaagaaa cctcattgct ggaagcctgg aaaaagtatc gggtgttgct gaaccgtgtt
     1921 gatacgtcaa ctgcacagga tattgaatgg ccagcactgc cgtagggtaa aacatataaa
     1981 ttctataatt agatgtatct ttccatttac ggcaaggaag ggggcttgga agacgtaaag
     2041 catctcacac cgagattatt ttttatatgt caggtgtctg aagttttgct ttggctctta
     2101 aaatggtttg ccgcgaggtt ttgaattccc gggcaatggc acttatactt acacctgact
     2161 taattcgttc gaataccacc tgtttctgtt cttcatttaa cacaggtggt cgaccaaaac
     2221 gtttccctgc gccgcgggct cttactatcc cggaatgagt gcgttcaagt aaaaggtctc
     2281 gttcaaattc agcgactgct gaaattacgt gcatcatcat ttttcctgtt ggactggtca
     2341 ggtcaatgcc ccccaatgct aagcaatgca ctctgatacc tatttcggtc agttgttcca
     2401 ctgttttcct gatatccatt gcattacaac caaggcgatc cagttttgtc acaatcaatt
     2461 gatcaccaca tttcaggcga gcaagcaacc ggttaaaacc aggacgctca ctggttgctg
     2521 ctgagccgct aatgtgttct tcgattattt gctgaggttt gattttaaaa cctgcacttt
     2581 cgatttcccg gcgttgattt tcgatggtct gatccagcgt tgatatccga cagtaagcaa
     2641 aaattcgaga catagtgaga ctctatacga aa
//
LOCUS       DAPHBJ010000302         2670 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.305, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000302 DAPHBJ010000000
VERSION     DAPHBJ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.305"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(63..1133)
                     /locus_tag="RHZ76_004411"
     CDS             complement(63..1133)
                     /locus_tag="RHZ76_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV2814912.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTVLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(1111..1329)
                     /locus_tag="RHZ76_004412"
     CDS             complement(1111..1329)
                     /locus_tag="RHZ76_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDV2814913.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(1369..1536)
                     /locus_tag="RHZ76_004413"
     CDS             complement(1369..1536)
                     /locus_tag="RHZ76_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814914.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYNHWMIWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            complement(1620..2048)
                     /locus_tag="RHZ76_004414"
     CDS             complement(1620..2048)
                     /locus_tag="RHZ76_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2513 domain-containing protein"
                     /protein_id="HDV2814915.1"
                     /translation="MKLDQQYLKDLLIAFEKTRGPDTMLSELEDNGFNRYDQDFIFHM
                     RLLCDYELIVRVDGKPGFGHILSKALGEGVGYSWIEVPLRLTARGHDFIADLRQKDVW
                     QAIKTNFKDEGISTLMSVSKSLAKGFARKKIKEITGIDIE"
     gene            complement(2077..2415)
                     /locus_tag="RHZ76_004415"
     CDS             complement(2077..2415)
                     /locus_tag="RHZ76_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814916.1"
                     /translation="MAKSATEHKADQRAKQASSGMRKLELVLDAQEIEMLERNCATRR
                     FRRAPCEFGEYIALLSRQDDARVRWRIKSISRKRCGKCGERVPVNSCPCNGDSQCWVT
                     KGWHETKLMI"
BASE COUNT          653 a          656 c          525 g          836 t
ORIGIN      
        1 aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
       61 cattatttga tttcaatttt gtcccactcc ctgcctctgt catcacgata ctgtgatgcc
      121 atggtgtccg acttatgccc gagaagatgt tgagcaaact tatcgcttat ctgcttctca
      181 tagagtcttg cagacaaact gcgcaactcg tgaaaggtag gcggatcccc ttcgaaggaa
      241 agacctgatg cttttcgtgc gcgcataaaa taccttgata ctgtgccgga tgaaagcggt
      301 tcgcgacgag tagatgcaat tatggtttct ccgccaagaa tctctttgca tttatcaagt
      361 gtttccttca ttgatattcc gagagcatca acatgcaata ctgttgggat ggcaattttt
      421 acgcctgttt tgctttgctc gacataaaga tatccatcta cgatatcaga ccacttcatt
      481 tcgcataaat caccaactcg ttgcccggta acaacagcca gttccattgc aagtctgagc
      541 caacatggtg atgattctgc tgcttgataa attttcaggt attcgtcagc cgtaagtctt
      601 gatctcctta cctctgattt tgctgcgcga gtggcagcga cagggtttgt tgttatatgg
      661 ccttcagcta ttgcctctcg gaatgcatcg ctcagtgttg atctgattaa cttggctgac
      721 gccgccttgc cctcgtctat gtatccattg agcattgccg caatttcttt tgtggtgatg
      781 tcttcaagtg gagcatcagg cagacccctc cttattgctt taattttgct catgtaattt
      841 atgagtgtct tctgcttgat tcctctgctg gccaggattt tttcgtagcg atcaagccat
      901 gaatgtaacg taacggaatt atcactgttg attctcgctg tcagaggctt gtgtttgtgt
      961 cctgaaaata actcaatgtt ggcctgtata gcttcagtga ttgcgattcg cctgtctctg
     1021 cctaatccaa actctttacc cgtccttggg tccctgtagc agtaatatcc attgtttctt
     1081 atataaaggt tagggggtaa atcccggcgc tcatgacttc gccttcttcc catttctgat
     1141 cctcttcaaa aggctacctg ttactggtcg atttaagtca acctttaccg ctgattcgtg
     1201 gaacagatac tctcttccat ccttaaccgg aggagggaat atcctgcact cgcgtaccca
     1261 tcgacgaact gtttcaaggc ttcttgggcg tcgctggcgt gcgttccact cctgaagtgt
     1321 caagtacatc gcaaagtctc cgcaattaca cgcaagaaaa aaccgccatc aggcggcttg
     1381 gtgttctttc agttcttcaa ttcgaatatt ggttacgtct gcatgtgcta tctgcgccca
     1441 tatcatccag tggttataac agtcgttgat gttctctgct tcgataaccc tgttgaatgg
     1501 ctctccattc cattcacctg tgactcggaa gtgcatttat catctccata aaacaaaact
     1561 cgccgtagcg agttcagata taatttccac caaaggcagt agttgcttga tgctaagaat
     1621 tattcaatat ctattcctgt aatctctttt atctttttcc ttgcaaagcc ttttgctagt
     1681 gattttgaaa cactcataag tgtactaatt ccctcatcct taaagtttgt ttttatagct
     1741 tgccagacat ccttttgacg taagtcagca ataaaatcat gccctcttgc tgtcaacctc
     1801 agtggtactt cgatccaact atatccaaca ccttccccta acgctttgga caatatatga
     1861 ccgaacccag gttttccatc aaccctgact attaattcgt agtcacataa taatcgcata
     1921 tggaaaataa aatcttggtc atatctatta aagccattat cctctagttc actaagcatc
     1981 gtgtcagggc cacgagtttt ttcgaatgcg ataagtagat cttttagata ttgctggtct
     2041 aatttcattg ccgcctccgt gacatgtcac agagatttat atcattaatt ttgtttcgtg
     2101 ccagcctttg gtcacccagc attgtgagtc accattacac gggcatgaat taacaggaac
     2161 tctctcgccg cacttaccgc accgttttct gctgatcgat tttatacgcc agcgcacacg
     2221 tgcatcatcc tggcggctca gtaacgcgat gtactcacca aactcgcaag gcgcacgcct
     2281 gaagcgacgc gtggcacagt tacgctccag catttcaatt tcctgagcat caagtacaag
     2341 ctccagctta cgcataccgg atgatgcttg cttggctctc tgatcggctt tgtgctctgt
     2401 tgctgattta gccattctgc ttttcctgca tcaggagaaa gacaatcatg gcggcgcgga
     2461 tacttccggt atctttatac gcgatattcc ccttcaatgg cacatacaag aagagagcca
     2521 tcaacaggag tgattgagga atcgacaacc agcaacgctt aattatctat agtgtcgtat
     2581 ttgcttcata aaatatggcg aataacacaa agcccgcagc agctcattgt gcgggctttt
     2641 cttataccta cttcctagag ataattctat
//
LOCUS       DAPHBJ010000303         2660 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.306, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000303 DAPHBJ010000000
VERSION     DAPHBJ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.306"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            10..1062
                     /locus_tag="RHZ76_004416"
     CDS             10..1062
                     /locus_tag="RHZ76_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HDV2814917.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLASGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(1378..2580)
                     /locus_tag="RHZ76_004417"
     CDS             complement(1378..2580)
                     /locus_tag="RHZ76_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDV2814918.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
BASE COUNT          622 a          675 c          632 g          731 t
ORIGIN      
        1 gagtttaaag tgaacatata tatcgggtgg cttttcaaat taatcccttt gattatgggc
       61 ttaatttgta tcgcgttagg tggctttgtg ctggaaagtt cagggcaaag cgagtatttc
      121 gtcgcgggtc atgtgctgat ttctctggcg gccatatgcc tggcattatt cactaccgca
      181 tttattatca tttcgcagct cacgcgcggc gttaatacgt tttacaatac attgttcccc
      241 attattggct atgcggggtc aattatcacc atgatatggg gttgggcact gttagcaggc
      301 aatgatgtga tggcagacga gtttgtcgcc ggccatgtta ttttcggcgt tggtatgatt
      361 gccgcctgtg tatcgacggt ggcagcgtca tccggtcact ttctgctcat tcccaaaaat
      421 gcagcgggga gcaagagcga cggaacaccg gtacaggctt attcttcatt aatcggtaac
      481 tgcctcattg ccgttcccgt tttactcacc ctgctcggtt tcatttggtc tattacgctg
      541 ttacgtagtg ctgacataac tccgcattat gtcgcgggtc acgtattgct tgggttaacc
      601 gcaatctgtg cctgtctaat tggccttgtt gccacaattg tccatcaaac acgtaatacg
      661 ttttcaacta aagaacactg gctgtggtgt tattgggtta tttttctcgg ctcaatcacg
      721 gtactgcagg ggatatacgt cttagtcagt tccgatgcaa gcgcccgact ggcttccggc
      781 attattctta tttgcctcgg aatgatctgt tacagcatat tctcaaaagt ctggctactg
      841 gcactggtat ggagacgtac ctgttcgtta gccaacagaa taccgatgat tcccgtcttc
      901 acctgcctgt tttgcctttt cctggcatcg tttcttgcgg aaatggcgca gaccgacatg
      961 ggatatttta ttccttcgcg ggttttggtc ggtttgggag cggtatgctt tacgttgttc
     1021 tcaatcgttt caatattaga agcaggttct gctaaaaaat aattgcaacg taccggataa
     1081 aaccagcgtt gaccatttgc gtaacgctgg tttttcttag gcatcatgaa ataacgcagc
     1141 attaatgcat agtggttaag gataaaaaaa caacaaagtt actgtctttt tcagcctgtt
     1201 catattttat aaagatctgg ccactgggtg agttttcaat cacctttcct tccaccttat
     1261 attaagcctg gagggtttca gttcacgggc tcattagaaa ataatcacaa gatagccact
     1321 atcaaaatta gctcattttt aatgcggctc taataatttt catctttagg taacaggtca
     1381 ggacgacttc gctgcaatca aatcctgcgc acaagcccat gcactcgacc acgcccactg
     1441 gaagttatag ccccccagcc agccggtgac gtccatcact tcgccgatga agtacagccc
     1501 aggcactttg cgcgcttcca tcgtccgtga agagagttcg ttggtgtcca cgccgccgag
     1561 cgtcacttcg gcagtgcgat agccttcagt gccgttgggt tgtacgcgcc agtcggtcaa
     1621 tgtgctaatc agtgcctgtt ggtcacgcac gttgagctgt tttagcgaaa catccgggat
     1681 ttgcccgagt tgctgtaagc gttcaaccaa ccgcttcggt agatgaaccg ccagtgtgtt
     1741 tttcaggctc tgattcggat gtgcgttacg ctgctcattc aggaaggttt cgaggtccac
     1801 atcaggtagc agattgatac tgacaaattc acccggttgc cagtagcttg aaatctgcaa
     1861 caccgccggt ccagacaagc cgcgatgggt gaagagtaag ttctcacgga aaacgatgcc
     1921 gttttcagcg gtaatcacgg aaggcaccgc cacgcccgcc agcacctgta actcttcgag
     1981 caacggttta tgcagagtga atggcaccag acccgcacgg gtcggcagca cgttgaggcc
     2041 aaattgttcg gcaatcttat aaccaaacgg cgacgcgccc agccccggca ttgacagccc
     2101 accggtcgcg atgaccagct tttcgcaacc gacagtcatg ccgttcagtt caagcgtgaa
     2161 gcctgtttca tccttcgcca cactcagcac ttcgctacgc aatctgaagg ttacattgcc
     2221 cttctcgcac tcatccacca gcatgtcgac aatctgctgc gcggagtcat cgcagaagag
     2281 ttgtcctaac gttttctcgt gccaggcgat gccgtgttta ttgaccagat caatgaaatc
     2341 ccactgggta aaacgcgcga gtgcagactt acaaaaatgc ggattctggc tcagataagc
     2401 gcctggttcg acataaaggt tggtaaagtt gcagcgccca ccgccagaca taaggatttt
     2461 gcgccctggt tttttaccat tatcgatcag cagaacccgg cgtcctgcct gacctgccag
     2521 cgcagaacag aacatacccg ccgcaccagc gcctataata atggcatcaa acctttccac
     2581 gttgcgctcc tcttagaaaa aacgggcgtg aattgtaaag attcctcagt ggtcgcacca
     2641 gcatcaatat tactaaaagg
//
LOCUS       DAPHBJ010000304         2601 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.307, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000304 DAPHBJ010000000
VERSION     DAPHBJ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.307"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            102..1760
                     /locus_tag="RHZ76_004418"
     CDS             102..1760
                     /locus_tag="RHZ76_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007372656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDV2814919.1"
                     /translation="MKIVIVGGVAGGASAAARARRLSEDVSIVVFERGSDVSFANCGL
                     PYHIGGKIPLRQSLILKTPEDFKSRFNIDVRICHEVTSVDPVNKTVTVKNLTSGEVYS
                     EEWDRLLLSTGAAPVVPPLPGLQEEGVFTLRNLTDMDAILGWIEQHHVAHTTLVGGGF
                     IGLEVMEALSERGISVTLLEMGEQVMAPVDPEMASALHQEIRSHGVDLRLRTALTEVL
                     RTETGFRVALSEGGFLQTDMVILAIGVKPENSLATGAGLAVGKRGGISVNACMQTSIP
                     DIYAVGDAVETPDFVFQEPANFPLAGPANRQGRIAADNMLDRHSLYHGSQGTSICKVF
                     SLSIGSVGANEKQLKVHGTRYEKVYVHAADHAGYYPGATMISLKLLFSPDTGKILGAQ
                     ASGKKGVDKRIDVLSVAQRAGLTVNDLEHLELTYAPPFNSARDVVNQAGMVATNVMKG
                     DTVICHVSDVLQREPGSYCLLDIRSPAELKQFGEYPDALSIPLDSLRENLEKVPKDKE
                     IFIGCQSGLRGHVAYRILQAHGFRTYNLSGGFITWQAVTESISK"
     gene            2006..2470
                     /locus_tag="RHZ76_004419"
     CDS             2006..2470
                     /locus_tag="RHZ76_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HDV2814920.1"
                     /translation="MRNFVTRLRVWFPPPLILLLFFVTDVLTAIPRFTFDIVNIVLSV
                     LLTGVCITMILHTAWQMSTNGTTLNPLHPDRTTKLVTVGCYAWSRNPIYLGMSGLQLS
                     VALCFGSLVGILAVPLFMFVVARLHIHVEEVQLRKRFGLEWERYTQRVRRWL"
BASE COUNT          625 a          591 c          715 g          670 t
ORIGIN      
        1 aattttgcca tcgtgttcgt caggtctgca gcgtcctgcc ggcccgggga aacgattata
       61 tctggcaggc atttttgccc cgggtgaaca gagaggatgt aatgaaaata gtgattgttg
      121 gtggtgttgc cggcggggca tccgctgcag ccagagctcg tcggttatca gaggatgtca
      181 gtattgttgt gtttgaacgg ggaagcgatg tctctttcgc caactgtgga ttaccttatc
      241 atataggcgg aaaaattcct ttaaggcagt cactgattct gaaaaccccg gaggatttta
      301 aatcccgctt taacatagac gtccgtatct gtcatgaagt gacgtcagtg gatccggtta
      361 ataaaacggt gacggttaaa aatctgacct caggggaggt ctatagcgaa gaatgggatc
      421 gtctgctcct cagtaccggc gccgctccgg ttgtccctcc tttgcccggg ctacaggaag
      481 agggtgtctt tacgctgcga aatcttactg acatggatgc tatcctgggg tggattgagc
      541 aacatcacgt agctcacacc acgcttgtcg gggggggatt tatcggactt gaagtgatgg
      601 aggctctgag cgaacggggg atcagtgtga ccctgctgga gatgggggaa caggttatgg
      661 ctccggttga tcctgaaatg gcatctgccc tgcatcagga aatccggagt catggcgtgg
      721 atctgcgtct cagaacggcg ctcaccgagg tactgcggac agagacggga ttccgcgttg
      781 cactgtctga gggtgggttt ctgcagaccg acatggttat tctcgccatc ggggtaaaac
      841 ctgagaacag ccttgccaca ggagccgggc tcgcagtggg taaacgggga ggcataagcg
      901 tcaatgcctg tatgcagacc agtatccctg acatctacgc cgtgggggat gccgttgaaa
      961 ctccagactt tgtctttcag gaacccgcta attttcctct ggcagggccc gccaaccgcc
     1021 agggacgcat tgctgctgat aacatgctag atcgtcacag cctttaccat ggcagtcagg
     1081 ggacatccat ttgtaaggta ttttcgctga gtattggcag cgtcggtgcg aatgaaaagc
     1141 agctgaaagt tcatggcacc cgatacgaga aggtttacgt ccatgccgct gatcatgccg
     1201 gttactaccc gggggcaacg atgatcagtc tgaaactgct gttcagcccc gataccggta
     1261 aaattctcgg ggctcaggca tcagggaaaa aaggggttga taaacgcatc gatgtcctgt
     1321 ctgtcgcaca gcgcgcgggg cttaccgtca acgatctcga acatcttgag ctgacttatg
     1381 ctccaccttt taacagtgca agggacgtcg ttaaccaggc tggtatggtg gcgacaaatg
     1441 taatgaaagg agatacggtc atatgtcatg tcagcgatgt gttacaacgg gaaccgggca
     1501 gttattgttt acttgatatt cgctctcctg ccgaactgaa acaatttggc gaatatcctg
     1561 acgcgctatc cattccactg gattccctgc gggagaatct tgaaaaagtg cctaaagata
     1621 aagagatttt tatagggtgc cagagtggcc tgcgcgggca tgtggcatac cgtattcttc
     1681 aggcccacgg cttcaggaca tataatctga gtggtggctt tataacctgg caggcggtaa
     1741 cggagtcaat aagtaaataa gagatgaggt gccatacaca tggcaccctg aatcgccaca
     1801 ggtttaacag tgaagtggtc tgctgaattt gttcacctga acagaggtgt ggactgaccc
     1861 cgcaccgtta taaggacagt tatgaaaaaa ataacttttc agatcaggca tataatgtca
     1921 gattcatggt cactatagga atggcaagga ctggattttc attttgcagc catcaggctg
     1981 cttagttcag agataggtct gtgtaatgcg taacttcgtg actcgattga gagtatggtt
     2041 tccgccccca ctcatcttat tgctgttttt cgtcactgat gtgttgactg caatcccgcg
     2101 ttttaccttc gacatcgtga atatagtgct aagcgtgttg ctgaccggtg tttgtattac
     2161 catgatactg cacaccgctt ggcagatgag cacgaacggc acgacgctta atccgcttca
     2221 tccggataga accaccaagc tggtaactgt cggttgttac gcatggagtc gaaaccctat
     2281 ttaccttgga atgagcgggc tccagttatc cgttgctctc tgctttggca gcctggtggg
     2341 gatacttgct gtaccgttgt ttatgtttgt agtagccaga ttacatatcc atgttgaaga
     2401 ggtacagctt cgaaaacgtt tcgggttgga atgggaacgc tatacccaac gggttcgtcg
     2461 ctggttatag caaccccgtg gtgttgcagg agacaaataa cgtcgaaatc ataaagaaaa
     2521 gaattcatca tccttgagct tttatatcgt aatcgtaaac caggtgccgt atgtagccat
     2581 tgagccagtg ctggtacctt a
//
LOCUS       DAPHBJ010000305         2547 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.308, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000305 DAPHBJ010000000
VERSION     DAPHBJ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.308"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            331..474
                     /locus_tag="RHZ76_004420"
     CDS             331..474
                     /locus_tag="RHZ76_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HDV2814921.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            487..696
                     /gene="cspE"
                     /locus_tag="RHZ76_004421"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             487..696
                     /gene="cspE"
                     /locus_tag="RHZ76_004421"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDV2814922.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            862..1671
                     /gene="rlmA"
                     /locus_tag="RHZ76_004422"
     CDS             862..1671
                     /gene="rlmA"
                     /locus_tag="RHZ76_004422"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HDV2814923.1"
                     /translation="MSFSCPLCHQPLTREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVSQLRERLNEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1668..2234)
                     /gene="mntP"
                     /locus_tag="RHZ76_004423"
     CDS             complement(1668..2234)
                     /gene="mntP"
                     /locus_tag="RHZ76_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HDV2814924.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          681 a          599 c          611 g          656 t
ORIGIN      
        1 gttttatgag aaaaagtgat ataacttttt attcattgca tagcaaaaaa tgtgatattg
       61 cacgcactat gtaataactt ctcccactgg cctggaacaa ctgaacttat tgaactatgt
      121 tagaaactac gccagtttaa gtatctgcct gaactggcaa ggttaagcac aatgatatat
      181 cggcgcgtat tccgttgcat aagtgtgcaa aaaaagtgga agacgtatcg agatttgtgc
      241 gtctgatcga gacatgttta aaaatggctt gccataatta acgttgtatg tgataacaga
      301 tttcgggtta aacgaggtac agttctgttt atgtgtggca ttttcagtaa agaagtcctg
      361 agtaaacacg ttgacgttga ataccgcttc tctgccgagc cttatattgg tgcctcatgc
      421 agtaatgtgt cagttttatc tatgttatgc ctgcgggcga agaaaacaat ctaaggaatt
      481 tttcaaatgg caaagattaa aggtcaggtt aagtggttca acgagtctaa aggttttggc
      541 ttcattactc cggctgatgg cagcaaagac gtgttcgtac acttctccgc tatccagggt
      601 aatggcttca aaactctggc tgaaggtcag aacgttgagt tcgaaattca ggacggccag
      661 aaaggtccgg cagctgttaa cgtaacagct atctgatcga atccactgat ctgaagtgtg
      721 aatacgcttc aatctcgcta taaagcctcg tcgaatgcga ggctttttac tatgctttgt
      781 cttcgctcct ggcgttcgga tatttgcccg ccgcgtgatt cgcgttacac ttgcggcctt
      841 tagtatcctg ccggagttgt catgtcgttt tcctgtccac tttgccatca gcctcttacg
      901 cgtgaaaaaa acagctatat ctgtccccaa cgacatcagt ttgatatggc gaaagaaggg
      961 tatgtcaatc tgctgcccgt tcagcataaa cggtctcgtg atccgggcga cagcgcggaa
     1021 atgatgcaag cacgccgcgc attcttagat gccggacatt atcagccgct gcgtgatgca
     1081 attgtctccc aactgcggga acggcttaat gaaaaggcca cggcggtgct ggatattggc
     1141 tgtggtgaag ggtattacac acacgcattt gccgatgcgt tgcccgaaat caccaccttt
     1201 ggtctggatg tttcgaaggt agcgataaaa gcggcggcga aacgctatcc gcaggtcact
     1261 ctttgtgtcg cttccagcca ccgtttgccg ttttccgata ccagtatgga cgccataata
     1321 cgtatttacg cgccgtgtaa agcagaagaa ttagcacgag tagtgaagcc cggcggctgg
     1381 gtcattactg ccacgccggg accgcgacat ttgatggagc tgaaggggct gatttacaat
     1441 gaagtacatc ttcatgcacc tcatgcagaa caactggaag gttttacatt acagcagagt
     1501 gcggagttgt gttatccgat gcgtcttcgc ggtgatgaag ccgtcgcatt attgcagatg
     1561 acgccgtttg cctggcgtgc gaagccagaa gtctggcaaa cactggcagc aaaagaagtg
     1621 ttcgactgcc agacggactt taatattcac ctctggcagc gttcttatta accgtggaag
     1681 tgcgtccaga ggatctggac gccgatgccg atcagcacca gcccgccgag aatttccgct
     1741 tttttcccaa taattgagcc gataaagcga ccaaccatca tccctaatgt tgacataatc
     1801 aaggttgcac aaccaatggc caatgcggtc gcgataatgt tgacctgcag gaaagcaaga
     1861 ccaacaccca cagccatggc atccaggctg gtggcaatcg cggtggttac cagtagccag
     1921 aaaccgtgtc gacggcgcgg ctcttcatct tcatcatctg cgccacgaaa accctcaata
     1981 atcattcgcc cgccgaggaa tatcagcagc acaaacgcaa tccagtggtt ccattcaagg
     2041 acaaaccggc tggctaacat gcccattccc cagccgatca gcggcgtcag ggtttcgacg
     2101 gcaccaaaaa taaggccggt tcgcaatgct tcagaaaatt tcggtttatg gagggtggca
     2161 cctttaccga ttgatgcagc aaatgcatcc atcgacatac caaacgcaag aagaacagta
     2221 gcagtgatat tcatgacaat gtcctgaccg gggtatccat aaaacacatc atcgccccca
     2281 gcaaacatgc atacatgaac aacaagccgt gatggttttc gcgttcatgc aggcatagcg
     2341 attgatgtgc atatggtctc gcctgactga aagtattcag tccgtacgtg ccacgttttg
     2401 caacgagtat gttgacacgt acatttccgg aatctggaaa tcggctactc cccaaatgag
     2461 ggcgcaacct taacatattt tgagaatata aaacaactgt tggaatatat tatttcattg
     2521 ctttattgat aacgattttc atttaga
//
LOCUS       DAPHBJ010000306         2534 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.309, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000306 DAPHBJ010000000
VERSION     DAPHBJ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.309"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            56..1486
                     /locus_tag="RHZ76_004424"
     CDS             56..1486
                     /locus_tag="RHZ76_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDV2814925.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVATETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(1527..2459)
                     /locus_tag="RHZ76_004425"
     CDS             complement(1527..2459)
                     /locus_tag="RHZ76_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDV2814926.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
BASE COUNT          783 a          528 c          563 g          660 t
ORIGIN      
        1 tatttcagca taagtggata ttcaaagttt tgctgtttta tcagggaata tttatatgat
       61 acgtaagtca gctacaggtg ttattgttgc gttagccgta atctggggtg gtggcacatg
      121 gtacacaggt acgcaaattc agcctggtgt cgaaaaattt attaaagatt ttaacgatgc
      181 taaaaagaaa ggtgaacatg cctacgatat gacgttaagt tatcaaaatt ttgacaaagg
      241 cttttttaat tcccgttttc aaatgcaaat gacattcgat aacggtgcac ccgatctcaa
      301 tatcaagcca ggccagaaag ttgtatttga tgtggatgtt gagcacggtc cgttgcccat
      361 cacaatgtta atgcatggta atgtcatccc agcactggca gcggcaaaag tgaacttagt
      421 gaataatgaa ctgacacaac cgctatttat cgccgcgaaa aataaatcgc ccgtggaagc
      481 gacattgcga ttcgcgtttg gtggctcatt ctctacgaca ttagatgttg cccctgcaga
      541 gtatggaaag ttttcttttg gtgagggcca gtttactttt aatggtgatg gtagttcatt
      601 gtctaacctg gatattgaag gcaaagtcga agatattgtt ctgcaattat caccaatgaa
      661 caaagtaacg gcaaaaagtt ttaccattga ttctctggcg cgattagaag aaaagaaatt
      721 tccggttggt gaaagcgagt cgaaatttaa tcagattaac attatcaatc acggggaaga
      781 cgttgcccaa atcgatgctt tcgttgcaaa aaccatgctg gatcgcgtta aagacaaaga
      841 ttatatcaat gtcaatctga cctacgaact tgataagtta acaaaaggga atcagcaact
      901 cggtagtggt gagtggtcat tgattgctga atctattgat ccctcagcgg tgcgccaatt
      961 tatcatccag tataacattg caatgcagaa gcagcttgct gcacaccctg agttagcaaa
     1021 cgatgaagtt gctctgcaag aagtgaatgc tgcattgttc aaagagtatt taccgttatt
     1081 acaaaaaagt gagccgacca ttaaacaacc ggtaaaatgg aagaacgcac tcggcgaact
     1141 aaatgccaat ctggatatca gtattgccga cccagccaaa tcttcatcat ccacaaacaa
     1201 agatattaaa tcgctcaatt ttgatgtgaa gttaccgctt aatgtcgcca cagaaaccgc
     1261 aaaacagctt aatttatctg aaggaatgga tgcggaaaaa gcgcaaaagc gggctgataa
     1321 acaaatcagc gggatgatga ccttaggtca gatgtttcag ttaatcacga ttgacaacaa
     1381 taccgcctcg ctgcaactgc gttatacacc gggtaaagtt gtttttaacg gacaggagat
     1441 gagcgaagaa gaatttatgt ctcgtgccgg acgttttgtt cattaaaata ctcactcgcc
     1501 tctgctcatt cagaggcgag caaaatttac ttgtcctgcc gctgtgatga catcaacgac
     1561 gtcagaagtc ggtctttttc ctgccacagt gagttcagcc attgctgaaa atggcgctta
     1621 aagcttttgt cattgatgta gtcgccatgt aactcatcgg caataggctg taaatccaca
     1681 tgtacgacaa tccgcgtcag tttaccgctt aacatatcga agaacggctg acggttattg
     1741 tccggataac acagcgtaac attaagcagt ttatcgaatt gtttacccag tacatttagc
     1801 gccatcgcaa tgcctgccgc ctttggtggc aacaagtttt gaaaagtgga gtgggtttgc
     1861 tgatgttttt cttgcgtgaa acgggagcct tcaacgaaat tgacaatggt ggtgggatgc
     1921 aggcgaaact tttcacaaga acgccgagtg gtttcaacat ctttaccacg tcgctccgga
     1981 tgacgtaata aataagcgcg ggaataacgc ttcataaacg gcatatccag cgcccagcac
     2041 gccaggccaa gaaatggcac ccaggccagc tgttgcttga ggaaatattt attcatcgga
     2101 atgtgcttac gaaacagcac gcacagtacg acaatatctg cccaactacg gtggttacaa
     2161 ataagcagat accagttctt cttacttaac ccttccagcc cgtgaacttc ccattgcagg
     2221 tgtgggttca gatgcagtaa taccgccaga ccttcacacc agcaatacat cataaaatca
     2281 caaaaacgcg aaacctttcg ccaaatgact ggcacaggta gcaatagttt tacaatcccg
     2341 gcaatgatga tcgggacgga gcaaaaaata gtgaccaaaa tggtcaatac gatactcagc
     2401 aaaagggtta tcgcagcgag tattctcgtc ataatgaatt tattcaaaag gttagccata
     2461 gacagtgcgc taaagaagcg caaggcgcag attttagcag aaaacgaccc aaataacgga
     2521 tgatccttaa ggag
//
LOCUS       DAPHBJ010000307         2503 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.310, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000307 DAPHBJ010000000
VERSION     DAPHBJ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.310"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..168
                     /gene="hemP"
                     /locus_tag="RHZ76_004426"
     CDS             <1..168
                     /gene="hemP"
                     /locus_tag="RHZ76_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HDV2814927.1"
                     /translation="LTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDHDGQEYLLR
                     KTQAGKLLLTK"
     gene            complement(172..1608)
                     /locus_tag="RHZ76_004427"
     CDS             complement(172..1608)
                     /locus_tag="RHZ76_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HDV2814928.1"
                     /translation="MTLSFITRWRDELPATYTALSPTPLNNARLIWHNAELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLADDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPTLVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(1671..2384)
                     /gene="ydiV"
                     /locus_tag="RHZ76_004428"
     CDS             complement(1671..2384)
                     /gene="ydiV"
                     /locus_tag="RHZ76_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HDV2814929.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQIMHRSFEPFIRAIQAQISPCCNCIIVGGVDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT          679 a          646 c          573 g          605 t
ORIGIN      
        1 ctcacgcctg aaacggatgc caatcacaag accgcttccc cgcagcctat tcggcgaatt
       61 tccagccaga cactgttagg tccggatggc aaactgatta tcgatcatga cgggcaagaa
      121 tatctgctcc gtaaaactca ggcaggaaag ctgctgttga ccaagtagcc tttaactcga
      181 gcagctgact tccagccgtt taccccagtc aggtggacga ctgacatagt catcatctct
      241 gtcgctgaaa ggatttcgca acgcctcatg caggcggtgc aattccgtca tatcaccctt
      301 ttctgccgct tcaatcgccc gttgcgccaa ccagttgcgc aataccagag ttgggttaac
      361 gctttgcatc agttgctgac gctcactatc gctaacctcg tcttgttgca aacgccctcg
      421 ataacgggca aaccagtcat caaatgccgc acgatcaata aactcatcac gtagcggtga
      481 cgccgcgctg tgctgctcgg tcagactcag catgcggaat gtgcgggtat aatcgctgcg
      541 ctctcgcgcc atcagactga ataattcatt cagtagcgcg ttatcctctt tttgctccgt
      601 catgaagccc agtttctgcc gcatccgttg tccataatgc gtcaacaaaa cctgctgata
      661 gctgtccagg gcctcattca gggcatcaac ggcaacaaat ggtgacaatg tctgcgccag
      721 acgctgtaaa ttccacaatg cgacggcagg ttgattgtca aagctgtaac gcccttgatg
      781 atccgagtga ttacaaataa aaccgggttc gtaatcatca agaaaaccaa acggcccgta
      841 atcaagcgtc agccccagca gcgacatgtt gtcggtattc atcaccccat gtgcaaagcc
      901 gaccgtctgc cattgggcaa ttaacgatgc ggtacgtgcg acaacatcgt taaaccagag
      961 acggtatttg tcctcatcat ctgcaagatg tgaccagtaa tgacgaatgg caaagtcagc
     1021 caactgacga accttttccg gctcgcggcg atagtaaaaa tgttcgaaat gaccaaagcg
     1081 caaatgactt ggtgccacac gcatcagcat cgcgcctggc tctaccgttt ccctatacac
     1141 tggcgaatcg ctggtgacga tacttaacgc gcgggtcgtc ggaatgccca gataatgcat
     1201 cgcctcactg gcgagacttt ctcgtatcgt cgaacgtaaa accgcccgtc catcacccat
     1261 tcgcgaataa ggcgtcaggc cagcaccttt cagatgccag tccattgtag tgccatcagc
     1321 aagcagttgt tcgccgagta aaataccgcg cccatcaccc agttggcccg cccagacgcc
     1381 gaactgatga ccactgtaaa cctgcgccag tggtgacata ccaggcagta gggtttcgcc
     1441 gccccagaca cctgcgccat ttttaaacag cgacgatgga atactcagcg tgttagccag
     1501 ttcagcatta tgccaaatca gccgggcatt atttaaaggc gtaggggaaa gtgctgtata
     1561 ggttgctggc aattcatcgc gccagcgggt aataaaagac agggtcatag atcctcctgt
     1621 ttgatagtgt agacggttac tctcgtctta aacaccagca aacaggaggg ttatcgctga
     1681 accaacgtcg ttatctgatt tatgggaacg gcaggccaca ggcatccctg gagagcatgg
     1741 aaatcaaagg gggtaatctg tgccaatatt tctggcgtat caacgccccc cacgatgatg
     1801 caattgcaac aaggggaaat ttgcgcctgg atcgcacgaa taaacggttc gaaggagcgg
     1861 tgcatgattt gttgctgaat aaaactttta tccagcatga cgcgggtgaa aagaccatca
     1921 aaaaccgctt tcattgtgct attgcctgct cccaaattac ctaacacaag gggatacatt
     1981 tgcgataagg acaaaagatc gcgattatct tttccttcat tgagatgcgg gtaattctca
     2041 ttaatcaaca gttcaataaa cggatatttt aatagttcgc ccgcgtaatt atccctgtct
     2101 aataataatg tagccacttg tggcgttaaa ttaagccagg caaagagttt atgttgaata
     2161 aaaaaatgtt ggcatgattt tagtaattcc aactgctctg aaaataattg ccaatgttgc
     2221 tcctcggtaa gttgcgctat aacccgactc gtcgggatac gcaccgtgcc atcttcactg
     2281 gagaaatggg taattaactc aacgccgaca aggtcttgct ggttatctct tatcggcagg
     2341 aaatagcaat cagaatgata aagattctcc aaaaaaatct tcatggtaac cgtccctctc
     2401 gaaggatgtt tttcagtatc cggctaaaag ggatgaggcc ataagacata acaattacag
     2461 aaggagtaac tttcatttgt tccatgttaa ccactttttc agg
//
LOCUS       DAPHBJ010000308         2478 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.311, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000308 DAPHBJ010000000
VERSION     DAPHBJ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.311"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1246
                     /gene="frc"
                     /locus_tag="RHZ76_004429"
     CDS             <1..1246
                     /gene="frc"
                     /locus_tag="RHZ76_004429"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HDV2814930.1"
                     /translation="TPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDVTR
                     HQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAIDH
                     MGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPPLV
                     SAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRLDK
                     LGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQNWE
                     NTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIPCA
                     PVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEHTA
                     AVLQELGYSDDEIAAMKQNHAI"
     gene            1300..>2478
                     /gene="oxc"
                     /locus_tag="RHZ76_004430"
     CDS             1300..>2478
                     /gene="oxc"
                     /locus_tag="RHZ76_004430"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HDV2814931.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     N"
BASE COUNT          671 a          547 c          607 g          653 t
ORIGIN      
        1 aactccactt caaggaatta aagttctcga tttcaccggt gtgcaatctg gcccatcttg
       61 tactcaaatg ctggcctggt ttggcgctga cgttattaaa attgaacgtc ccggcgttgg
      121 tgacgtaacg cgtcaccagc tgcgagatat tcctgatatc gatgcgcttt acttcaccat
      181 gcttaacagt aacaaacgtt ctattgagtt aaataccaaa acagcggaag gcaaagaggt
      241 aatggaaaag ctgatccgcg aagctgatat cttagtcgag aactttcatc caggggccat
      301 tgatcacatg ggcttcacct gggagcatat tcaagaaatc aatccacgtc tgatttttgg
      361 ttcgatcaaa gggtttgatg agtgttcgcc ttatgtgaat gtaaaagcct atgaaaacgt
      421 tgctcaggca gcgggtggcg cggcatccac tacgggtttt tgggatggtc cgccgctggt
      481 aagcgctgca gcgttgggtg acagcaacac cggaatgcat ttgctgatcg gtttacttgc
      541 tgctttgctg catcgcgaaa aaacggggcg tgggcaacga gtcaccatgt caatgcagga
      601 tgccgtattg aacctttgcc gcgtgaaatt acgtgaccag cagcgtctcg ataaattggg
      661 ttatctggaa gaatacccgc agtatccgaa tggtacattt ggtgatgcag ttccccgcgg
      721 tggtaatgca ggtggtggcg gtcagcctgg ctggatcctg aaatgtaaag gctgggaaac
      781 cgatcctaac gcctatattt atttcactat tcaggagcaa aactgggaaa acacctgtaa
      841 agccatcggc aaaccagaat ggattaccga tccggcatac agtacagccc atgcacgaca
      901 gccacatatt ttcgatattt ttgctgaaat cgaaaaatac actgtcacta ttgataaaca
      961 tgaagcggtg gcctatttga ctcagtttga tattccttgt gcaccggttt taagtatgaa
     1021 agaaatttca cttgatccct ctttgcgcca aagtggcagt gttgttgaag tggaacaacc
     1081 gttgcgtgga aaatatctga ccgttggttg tccaatgaaa ttctctgcct ttacgccgga
     1141 tattaaagct gcgccgctat taggtgaaca taccgctgct gtattgcagg agctgggtta
     1201 tagcgacgat gaaattgctg caatgaagca aaaccacgcc atctgatatt aacgggggct
     1261 tctggctccc aatttataaa aatttcgagg ttattaatca tgtcagatca acttcaaatg
     1321 acagatggta tgcatatcat cgttgaagca ttaaaacaga ataatattga cactatttat
     1381 ggtgttgtag gtattcctgt gacggatatg gcacgccatg cccaggcgga aggcattcgt
     1441 tatattggtt ttcgtcatga gcagtcggca ggctatgccg ctgcggcaag cggttttctt
     1501 acccaaaaac cggggatctg cctgacagtt tctgcgccag gattcctcaa tggtttgacc
     1561 gcattggcca acgcaacggt aaatggtttt ccgatgatca tgattagcgg ctccagcgac
     1621 cgcgcgatcg tcgacctaca gcaaggtgat tatgaagagc tggaccaaat gaatgcggca
     1681 aaaccgtatg ccaaagcagc atttcgcgtt aatcagccgc aggatcttgg cattgcattg
     1741 gcacgcgcta tccgggtctc tgtatcgggt cgccctggcg gagtttatct tgatttgcca
     1801 gcaaatgtcc tggccgcgac gatggaaaaa gacgaagcgt taaccacgat tgttaaagtt
     1861 gaaaatccgt cgccagcatt attgccatgc ccgaagtcag tcactagcgc aatttcgctt
     1921 ttagcaaaag ctgaacggcc attaattatc cttggcaaag gcgcggcgta ttcacaagct
     1981 gatgaacagc ttcgtgaatt tattgaaagt actcagattc cattcctgcc aatgtctatg
     2041 gcgaaaggga tccttgaaga tacgcatcca ctttctgcgg cagctgcgcg ttcgtttgcc
     2101 ctggcaaatg ctgacgttgt catgcttgtt ggtgcacgac tgaattggtt attggcacac
     2161 ggtaaaaaag gatgggcggc agatacacag tttattcaac tggatattga accgcaggaa
     2221 attgacagca accgccccat tgctgtgcca gtcgttggtg atattgcatc cagtatgcaa
     2281 ggtatgctgg cagaactgaa acaaaacaca tttacgactc cactggtatg gcgcgatatt
     2341 ttaaatatcc acaagcagca aaatgcacaa aaaatgcatg aaaaattaag tacagatact
     2401 caaccattaa attactttaa tgcattaagt gctgtgcgcg acgtattgcg cgagaaccag
     2461 gatatttatt tagttaat
//
LOCUS       DAPHBJ010000309         2424 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.312, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000309 DAPHBJ010000000
VERSION     DAPHBJ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.312"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            72..2351
                     /gene="ydeP"
                     /locus_tag="RHZ76_004431"
     CDS             72..2351
                     /gene="ydeP"
                     /locus_tag="RHZ76_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HDV2814932.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKNGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
BASE COUNT          621 a          578 c          625 g          600 t
ORIGIN      
        1 atccctttgt atgtactgtg catcatcgct attacaaatc ctaataattc atttccacac
       61 aggataagta gatgaagaaa aaaattgaat cctaccaggg tgctgccggt ggttggggtg
      121 ctgttaaatc cgtagcgaat gcagtacgta agcagatgga tatacgccag gatgttattg
      181 ccatgtttga catgaataag ccagagggct ttgactgtcc gggttgtgca tggccagatc
      241 ctaagcacag tgcgtcattc gacatttgtg aaaacggcgc aaaagcaatc gcctgggaag
      301 tcacggataa gcaggtaaac gcctctttct ttgctgagaa tacggttcaa tcattactta
      361 cctggggaga ccacgagctt gaggctgcgg ggcgactcac tcagcctttg aaatatgatg
      421 ccgtcagcga ctgttacaag ccattaagct ggcaacaagc tttcgacgaa attggcgcac
      481 gccttcaaag ctatagtgat cccaatcagg ttgaattcta tacttcgggc cgcacttcca
      541 atgaagctgc ctttctttat cagctttttg cccgtgaata cgggagcaat aactttcccg
      601 actgctccaa catgtgccat gaaccgacaa gcgtgggttt ggcagcgagt atcggtgtag
      661 gtaaagggac cgtgttgctg gaagactttg agaagtgcga tttagtcatt tgcattgggc
      721 ataaccctgg tacaaaccac cctcgcatgc tgacttcgtt gcgcgcttta gtgaaacggg
      781 gagcgaaaat gatcgccatc aatcctctac aggaacgtgg cctggagcga tttaccgcac
      841 cgcaaaaccc gtttgaaatg ctgacgaact ctgagactca gttggccagt gcctactata
      901 acgtgcgcat tggtggcgat atggcgttgc tcaaggggat gatgcgcctg ttaattgagc
      961 gcgatgatgc tgcaagcgcc gcaggtcggc cttcattgct tgatgacgag tttattcaaa
     1021 cgcataccgt cggctttgac gagctacgcc gtgacgttct caattccgag tggaaagata
     1081 tcgaacgtat ttctggacta agtcagacac aaatcgccga actggctgac gcatatgccg
     1141 ctgccgaacg caccattatc tgttacggaa tggggatcac tcagcacgaa catggtaccc
     1201 agaacgtaca gcaactggtc aatctgctgt tgatgaaagg taacattggc aagcctggtg
     1261 cgggtatctg cccactacgt ggacactcta atgtacaggg cgaccgaacc gtcggtatca
     1321 ccgagaaacc gtctgcagag tttctggctc gtctgggtga gcgctatggc ttcaccccac
     1381 ctcatgcacc tggacatgct gcaattgcca gcatgcaagc aatatgtacg gggcaggctc
     1441 gagcattgat ctgcatgggg ggcaattttg cgctggcaat gccagatcgg gaagcgagcg
     1501 ctgtaccgtt aacgcaatta gatttggcgg tacacgtagc cactaagctt aaccgctctc
     1561 atctgttgac cgcacggcat agctatattc tgccggtcct gggacgtagc gagattgaca
     1621 tgcaaaaaaa cggtgcgcag gcggtaaccg ttgaggattc aatgtcgatg attcatgcct
     1681 cgcgtggcgt gttaaaaccc gccggtgtaa tgctgaaatc agagtgtgca gtggtcgcgg
     1741 gaatcgcgca ggcagcacta ccccagagcg tggtagcctg ggagtatctg gtggaagatt
     1801 atgatcgcat tcgcaatgac attgaagctg tgctgccaga gttcgccgac tataaccagc
     1861 gcatccgtca tcccggtggt tttcacctga taaatgcagc tgctgaaagg cgctggatga
     1921 cgccgtcagg taaggctaat ttcattacca gcaaagggct gttagaagat ccctcttcag
     1981 cgtttaacag taagctggtc atggcgacag tacgcagcca cgatcagtac aacacgacga
     2041 tttatggtat ggatgatcgc tatcgagggg tattcggtca acgagatgtg gtctttatga
     2101 gtgctaaaca agctaaaatt tgccgtgtaa aaaacggcga aagagttaat cttattgcgc
     2161 ttacgccaga cggtaagcgc agctcacgcc gcatggatag attaaaagtg gtcatttacc
     2221 ctatggctga ccgctcactg gtgacctatt ttccagaatc gaatcacatg ctaacacttg
     2281 ataaccacga tccattaagt ggcattcctg gctataaaag tattccggtt gaattagaac
     2341 catcaaatta atgcctcttc tcatttcttc tgctgtcatc cgcacagcag aagaattcct
     2401 cattgactat tatttcgcaa tttg
//
LOCUS       DAPHBJ010000310         2421 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.313, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000310 DAPHBJ010000000
VERSION     DAPHBJ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.313"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            465..692
                     /locus_tag="RHZ76_004432"
     CDS             465..692
                     /locus_tag="RHZ76_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814933.1"
                     /translation="MQAAPQREETEWRVQSKRGLMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            complement(727..867)
                     /gene="ykgO"
                     /locus_tag="RHZ76_004433"
     CDS             complement(727..867)
                     /gene="ykgO"
                     /locus_tag="RHZ76_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HDV2814934.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(867..1130)
                     /locus_tag="RHZ76_004434"
     CDS             complement(867..1130)
                     /locus_tag="RHZ76_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDV2814935.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGS"
     gene            complement(1493..1594)
                     /locus_tag="RHZ76_004435"
     CDS             complement(1493..1594)
                     /locus_tag="RHZ76_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814936.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
BASE COUNT          707 a          451 c          533 g          730 t
ORIGIN      
        1 ctttgccctg aagtgttagg aatagtctta atgcgctgtg tgatggacta gtaaaaaaac
       61 aagttttaag ggtaagggat agaatataaa actatgatga gctgtcattg aagatgattt
      121 gccaatcagt aagatatttt aatggattaa catgccgcac aaaatggtta caggtgttca
      181 ttccaataag taatggaaaa ttgaacgggc gtatgtacgt catctgttcg gtaagccccc
      241 aagagccaga ttaagtgaag ttaaacgaag gctgccagct gtcacaggaa cgatggcctt
      301 tttgtcaggg cactgcattc agttatctgt gcgcgttact ctaactggca ctcgaacgtc
      361 ggcgacgggg ttaccggtgg atcagactgt tcctgtggcg taaacgtttt tatgttagta
      421 ccaacagatt taccacattt atcatcttaa cagtttgagt gaaaatgcag gctgcgccgc
      481 aaagggagga gactgagtgg cgtgttcagt ctaaaagagg gttgatgcca gcatacaggg
      541 gcgaagctgg tcaacaagtc aacattaaga taatggaata ttcggagaga aacgtcaggc
      601 agttggcctc aaatgaacag gaagaatata tccccaggaa aattaatgtt ggtgttataa
      661 acacaccaac attaatccga tctgattatt aatcagacaa atatcgttaa tccgtcgaat
      721 tttgaatcaa cgttttttct tacgaccctg aacggcctta aaacgtggat tagatttaca
      781 aatcacatat agccgtcctt ttcgcttcac aatctgacag tctggatggc gttcttttgc
      841 ggtacgcaga gagttaagaa ctttcatcac gacccctttt tcgtgctaac aaaacgacca
      901 aaacgttggg tgaatcgtgc aacatttcct tctgatgcca ctgttctcag cttccctgta
      961 tagaacgggt gcgatttaga agagacatca attgtcacgt atggatacgt tacgccatcc
     1021 agctcaatct cacggtctgt tttgatagtc gagccgattt taaagtactc atcaacactg
     1081 gtgtcgtgga acaccacagt acgatactca ggatggatat tgggcttcat catttttacc
     1141 tgttatgtta taacataacc ataaagatac atgctcatcc ggatcatttc aacacccaat
     1201 ccctgtgact ctttgtggat gtggaatttt cgcaacgggg aactctggca ttgttagcag
     1261 ccgttggtga cgctcccata aacgcctgga tgagtgattg atgatggttt tgctgagagt
     1321 atggattttt cgagaatgga ggttatctcc actttactgg ccgatgattt atcgcagtat
     1381 gattgattac ctgacagcgt gtattcctct cccacggata taataattac ttacaaaaag
     1441 ggagaggatg catattttaa atatcactga agtgaacagt ttatttccgt tattaataga
     1501 aatggagaaa taaataggcg tattctacaa ttgcgacaaa aacaacgata ttaatcagtt
     1561 tatgactgat ttgctgtact ttattctctt tcattggtac ttcctcgctt taaaaaagag
     1621 tgcacttcgt aagtgccctt atataaataa cgagtttggt caaccaattt tttgacatgt
     1681 atcacaaatt tgaatagatg tattacatca actatctttt attgcaccaa cgtcattgat
     1741 atatgtcgcc tgaagtcagt tccgggaatg agtctgatct caagactggc ccagtccggg
     1801 cgttgattgg tgctgaggag catatcgcat ctcatcataa tgtcgtatct cctggggtgt
     1861 tatacaagat atcgttgttg gtgacctggg agaggaattg agttctatta aaccgtcaac
     1921 tatgccggat acatactgga ttacactgca ggcacgcctt atgagagaac gtgccgcagt
     1981 gacgggttaa ttatctgaaa gaatttgtga ggctgtatcg gttactcatt gatttgatag
     2041 ttttactctc gggagaataa tagatattta atccattaac ggaaaccagc cagttccttt
     2101 cgatgcctga atttgatccc atagtttatc cggaatggtt aattcatgaa caagttgtgt
     2161 tatcgttcat gagaagcata acgtaaaggg aaaagctcga ttagacggca gaatttgtca
     2221 ggggttatga acgaaattca taaatctgtt tgagtgttgc gatgggtagt gcaagttcga
     2281 tatctccgca atttacagtc cgatgaagga aaatgaatat ccataaaaat atattggttt
     2341 atcctggcat atatacctat ttcgacgtat ttccaatagt tttaattaaa ggcaggtcat
     2401 tgttattcac tctgaatagt g
//
LOCUS       DAPHBJ010000311         2324 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.314, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000311 DAPHBJ010000000
VERSION     DAPHBJ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.314"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            37..450
                     /gene="hiuH"
                     /locus_tag="RHZ76_004436"
     CDS             37..450
                     /gene="hiuH"
                     /locus_tag="RHZ76_004436"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HDV2814937.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVIFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            559..1563
                     /gene="msrP"
                     /locus_tag="RHZ76_004437"
     CDS             559..1563
                     /gene="msrP"
                     /locus_tag="RHZ76_004437"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HDV2814938.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKALEFSKPAAWQNNLPLTPVDKVSGYNNYYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            1564..2199
                     /gene="msrQ"
                     /locus_tag="RHZ76_004438"
     CDS             1564..2199
                     /gene="msrQ"
                     /locus_tag="RHZ76_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HDV2814939.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
BASE COUNT          596 a          569 c          558 g          601 t
ORIGIN      
        1 atcctcaaaa ctcattcaca tgacaaggat ataaacatgt taaagcgtta tttagtactc
       61 tccgtagtaa cggctgcatt ttcattacct tctttggtct atgctgcaca acaaaacatt
      121 cttagcgtgc acattttgaa ccagcaaaca ggaaaacccg ctgccgacgt gacagtcact
      181 cttgaaaaga aagcggacaa cggctggtta caacttaata ccgccaaaac agataaggat
      241 ggacgaatta aggcactgtg gcccgagcaa actgcaacta cgggcgatta ccgtgtcata
      301 tttaaaaccg gggactattt caagaaacaa aatcttgaaa gtttcttccc tgagatcccc
      361 gttgaatttc atattaataa agtgaacgag cattatcatg tgcctttatt acttagccaa
      421 tatgggtatt caacctatcg tggcagttaa tgcataaata tgtaaaagca gatctctgct
      481 actgttaggg aggacactcc ctgacagatt aacagtaaac ggctcttgct ggctaacgac
      541 aaaaaagtgt gatggcttat gaaaaagaat caatttttaa aagaatcaga tgttacggcc
      601 gagtcggtat tctttatgaa gcgtcggcag gtgttaaaag cactgggcat cagcgcagct
      661 gcactttctt tgcctcacgc tgcgcatgcc gatctgctta gctggtttaa agggaacgat
      721 cgcccgcccg cccccgccgg aaaagcgctg gagttcagca agcctgccgc ctggcaaaat
      781 aatctgccac tgacgccagt agataaagtc tccggttata acaactacta tgaattcggg
      841 ctggataaag ccgatcccgc cgctaatgct ggtagcctga aaaccgatcc atggacactg
      901 aaaatcagcg gcgaagtggc aaaaccattg accctcgatc acgatgattt aacccgtcgc
      961 tttccgctgg aagagcgtat ttatcgtatg cgctgcgtag aagcatggtc gatggtggtg
     1021 ccgtggattg gttttccgct gcacaaattg ctggcgcttg ccgaacccac cagcaatgcg
     1081 aagtatgtcg ctttcgaaac aatttatgca ccggaacaga tgccaggcca gcaggaccgc
     1141 tttatcggcg gcgggctgaa atatccttat gtcgaaggat tgcgtctcga cgaagcaatg
     1201 catccgctca cactgatgac cgtgggtgtt tatggcaagg cgttaccgcc acaaaatggc
     1261 gcgccggtgc gactgattgt gccgtggaaa tatggcttta aagggattaa atcgatcgtc
     1321 agtattaagc tgacccgcga gcgtccgcca accacctgga atctggcagc gcctgacgaa
     1381 tacggttttt acgccaacgt taatccgcat gttgatcacc cgcgctggtc acaggctacc
     1441 gaacgattta ttggttcagg cggcatcctc gatgtacagc gccagccaac gctactgttt
     1501 aatggttacg ccgaccaggt ggcatcgctg tatcgtggcc tggatttgcg ggagaatttc
     1561 tgaatgcgtc tgacagcaaa acaggtgaca tggctgaaag ttagcctgca tcttgccgga
     1621 ttgttgccgt ttctctggct ggtctgggcg atcaatcacg gtggactggg tgccgatccg
     1681 gtgaaagata ttcagcattt taccggtcgc actgcactga aatttttgct ggcgaccttg
     1741 ttaatcacac ctctggcacg ctacgcaaaa cagccgttat tgatacgcac tcgccgcctg
     1801 ttaggcttat ggtgctttgc ctgggcgaca ctgcatttaa ccagttacgc attgctggag
     1861 ctgggcgtga ataatctggc gttattaggc aaggagttaa ttacccggcc ttatttaacg
     1921 ttaggaatta tcagctgggt tattttgctt gctttagcgt ttacttcaac ccaggcgatg
     1981 cagcgaaaac tcggcaagca ctggcaacag ttgcataatt tcgtctatct tgtcgcgatc
     2041 ctggccccaa tacattatct gtggtcagtg aagattattt caccgcagcc gctcatctac
     2101 gctgggctgg ctgtactgct tttagcctta cggtataaga agttgcgttc cctttttaac
     2161 cggttacgca agcaggttca caacaaatta tctgtataac ttcatattgc cgacaaagta
     2221 cgcgttatcc ctgccggatg tggcgcgagc gccctgtccg gcctgcgcag atcacatcag
     2281 gttatgtttc gtttgtcatc aacctcaaat tatcaacata attc
//
LOCUS       DAPHBJ010000312         2324 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.315, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000312 DAPHBJ010000000
VERSION     DAPHBJ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.315"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            49..678
                     /locus_tag="RHZ76_004439"
     CDS             49..678
                     /locus_tag="RHZ76_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDV2814940.1"
                     /translation="MCEHLITVVEKYGAAERVHLGKQAGNVGRAVTKLPLMGKSLHKT
                     IERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLACRRRGLIVLTDRYPQDQIPGA
                     YDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNVDLEVACARKPDHKRE
                     SLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAITDFMTARGYH"
     gene            complement(727..2226)
                     /gene="pitB"
                     /locus_tag="RHZ76_004440"
     CDS             complement(727..2226)
                     /gene="pitB"
                     /locus_tag="RHZ76_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HDV2814941.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
BASE COUNT          622 a          591 c          568 g          543 t
ORIGIN      
        1 cctccattaa ttgccgttat tggtagcgat ggttcaggca agtcaacggt gtgtgaacat
       61 cttattaccg ttgtcgaaaa atatggtgct gccgaaagag ttcatttagg aaaacaggcc
      121 ggaaatgtcg gtcgtgcagt gacaaaatta ccgttgatgg gaaaatcctt acataaaaca
      181 attgaacgaa atcaggtgaa aacagcaaaa aaattgcctg gaccagttcc ggcgctggta
      241 attacagcgt ttgtcgcccg tcgcttactg cgctttcgtc atatgcttgc ctgtcgtcgt
      301 cgcgggttaa ttgttctaac cgaccgttat cctcaggacc aaattcctgg cgcttacgat
      361 ggtacggtgt tcccacctaa cgttgaaggt ggtcgttttg tctcatggct ggcaagccag
      421 gaacgtaaag cgtttcactg gatggcgagc cataagcctg atctggtcat caaactcaat
      481 gttgaccttg aagttgcctg tgcacgtaaa cccgaccata aacgggaatc gctggcgagg
      541 aagattgcca taacgccaca gttaaccttt ggtggtgcac aactggttga tatcgatgcc
      601 aatcagccac tggaacaggt gttggttgat gcagaaaaag cgattacgga ttttatgacc
      661 gcgcgtggtt atcactagtc aaaaatggaa atgcccgatc gccaggaccg ggcattttca
      721 ggaaggttaa atcaactgca atgctatcca gtacagccca ccagaaagaa aaattgccgc
      781 cggtaaagta aatacccacg ccatcaggat gctggttacc gttttacgct gtaacccacc
      841 gccgtccacc accatcgtcc ctgcaactgc agacgagagg acgtgtgttg tggagacggg
      901 catcccaata taactggcaa gaccgataga cactgccgcc gtcatttgtg ccgccatgcc
      961 ttgcgcatac gtcatgccgc gcttaccaat cttctcaccg atggtcatcg ctacacgacg
     1021 ccagccaatc atggtgccaa tgccgagcgc cagtgctacc gccatgatga tccacaccgg
     1081 agcgtactca atggtgctta acatatcgct gcgaagtttt ttcagcaggt tctggtcttc
     1141 tttactgacg cctggcagtt tcgctagctt cgcggaggta tcagagatgc acagcataat
     1201 gcggcgcagc tggctgcgct gactcacgct taacggctcg taactttcca tattgcctgg
     1261 cagcatcgtt ttaacgcgcg caatagcatc aaaggtattt gccggatgac agtgaaactc
     1321 tgttacttgc gtgccatcag tcgatgctgc aggcaatgga ggttccatcg caatcaactt
     1381 ctgcggcagt tcaggatgct gttgcaggta gtgttcgaag ttggtaacgg catcgcgggt
     1441 acgggtaatt tcatagccgg acgcattcat attgacgacg aagccagcag gggcaatccc
     1501 caccagtacc agcattacca ggccgatccc tttttgtccg tcgttcgcgc cgtgcgaaaa
     1561 cgccacgccc gcagcggaaa caatcagcgc aatacgcgtc cagaatggcg gtttacgttt
     1621 gccttttttc tttttgcgat cttccggaat gcggtgaata cggtcacgct ttttcgtccc
     1681 gctccagtag cgtcgcagca ggaatatcag gcctcccgca atgaccaggc cgacgatagg
     1741 ggaaacaatc agcgaggaga aaattttggt cacttcacgc aggtttaacg catccatcac
     1801 cgatgagccg gttaacagcg cgttggttaa accgatgccg ataatcgcac caatcaaggt
     1861 gtgcgaactg gaggccggta aaccgaagaa ccacgttccc aggttccaga taatcgccgc
     1921 cagcagcatg gaaaagacca tcgccaggcc gtgggttgac cccatattca gcaacaaatc
     1981 ggttggcaac atatggacaa tggcataggc aacgctaagt ccgcccaata acacgccaaa
     2041 aaagttaaaa aatgccgcca tcaccacagc aagttgtggt tgcatggcac gagtataaat
     2101 aacggttgcc accgcattcg ccgtgtcatg aaaaccattg attgcttcgt agaacaacac
     2161 aaatgccaga gcaagcaata acaaaagccc tgtgtatata tcaaggccaa caaataaatt
     2221 tagcatatat tagattacgc cattttgaat ttacgaacgg acgcattatc agtgacttta
     2281 acggcatggg caaagtgaaa tatcattttt taacgataaa aaga
//
LOCUS       DAPHBJ010000313         2322 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.316, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000313 DAPHBJ010000000
VERSION     DAPHBJ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.316"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            3..785
                     /locus_tag="RHZ76_004441"
     CDS             3..785
                     /locus_tag="RHZ76_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013202940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2814942.1"
                     /translation="MIIQKRELIWQMSKRDVMGRYKGSFLGLAWSLFNPILMLTVYTF
                     VFSVIFKSRWGSSPDAGHADFAIILFTGLIIFNLFAECINRSTSLITSNVNFVKKVVF
                     PIEILPIVNLLAALFHASISLIVLFIAILVFKHQLHFTVLLLPVITIPLMLSILGLSW
                     ILASLGVFVRDIPQTISIIVTILMFLSPVFYPLSALPVVFQKIVMLNPLAFMIEEARK
                     VVYWGNEPNWTMLAINMLIGLVICAAGFLFFQKTKKGFADVI"
     gene            775..2184
                     /locus_tag="RHZ76_004442"
     CDS             775..2184
                     /locus_tag="RHZ76_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2814943.1"
                     /translation="MSSDNIAISVSNISKCYNIYQNPRDRLKQFFLPKVQSMVGKPVQ
                     RYYNEFWALNNINFTINKGESAGIIGRNGSGKSTLLQLITGTLTPTTGTIQTKGRIAA
                     LLELGSGFNPEFTGRENIYLNGAVLGLSRAEIDAKFDAIAGFADIGTHLDQPVKTYSS
                     GMMVRLAFAVQVQLEPDILIVDEALAVGDALFQKRCFQQIEKLTSNGVTLLFVSHDQE
                     SVRTLTNRAILLDRGTQLSTGLSSEVLLEYRRLLHREESKYFSQLAKEVAAHADATRD
                     INQTPTAPAKIEISANAETPAVEETSPSRTDKLSFGEGEAEVENVELFTASGERSNAF
                     NPGDKIRVRVNCKVNKDINHINVGIRIRNKEGVKIYSWGTLNQDMYVRFHQLKDPQFW
                     EREFKAGERFYVDMEFECTLGTNLYEIQAAISYEGTPNYFSQRILHWRDEAAFFHVNV
                     LRDEYFFGGILDLKMTAEW"
BASE COUNT          681 a          456 c          483 g          702 t
ORIGIN      
        1 agataattat ccagaagcgc gagttaatct ggcaaatgtc taaaagagac gttatgggac
       61 gctataaagg atcctttctt ggtctggcat ggtcactttt caaccctata ttgatgttaa
      121 cagtctacac atttgtattc tcggttatat tcaaatctcg ttggggaagc agtccagatg
      181 ccgggcatgc agattttgca atcattttgt ttaccggact gattattttt aacttatttg
      241 cagaatgtat taacaggtca accagcctta taacctcaaa tgtaaacttc gtcaaaaaag
      301 ttgttttccc tattgagata ttacctatag taaatctgct ggctgcttta tttcatgcca
      361 gcattagcct catagtctta tttattgcaa tactggtgtt taagcatcag cttcatttta
      421 ctgtcctgtt actgccagtc attactattc cacttatgct ctctatactt ggattaagct
      481 ggatcctggc ctcactgggc gtttttgttc gtgatatacc acaaactatt agtatcatcg
      541 taacaatatt aatgttttta tctcccgtat tttatccgtt atcagcatta ccggttgttt
      601 tccaaaaaat tgtaatgttg aatcctctgg catttatgat tgaagaagca agaaaagttg
      661 tttactgggg aaatgagcct aactggacca tgctcgcaat taatatgctt attggtttag
      721 ttatctgtgc tgcaggtttt ttatttttcc aaaaaacaaa aaaaggattt gccgatgtca
      781 tctgataaca ttgcaattag cgtaagtaac attagcaaat gctataacat ttaccaaaac
      841 cccagagata ggttaaaaca gtttttctta cctaaagtcc agagtatggt tgggaaacct
      901 gtacaacgct actataacga attttgggcg ttaaataaca tcaactttac catcaataaa
      961 ggtgagtcag cgggaattat tggacgcaat ggttctggca aaagtacatt actacagcta
     1021 atcaccggca cgctaactcc aacaacgggc acgatacaaa ccaagggaag aatagctgcc
     1081 ctcctggaat taggctccgg tttcaaccca gagtttaccg gtagggaaaa catctatctt
     1141 aacggcgccg ttctgggatt atcgcgggca gaaatagatg caaaatttga tgctattgcc
     1201 ggttttgctg atataggaac ccatctcgac cagccggtaa aaacatactc cagcggaatg
     1261 atggtgcgcc ttgcatttgc cgttcaggta caattagagc cagatattct catcgtagat
     1321 gaagcacttg ctgtaggcga cgcacttttt caaaagcgct gtttccaaca aattgagaaa
     1381 ttaaccagca atggcgtaac tttactgttt gtttcacacg atcaagagtc cgtgcgtacc
     1441 ttaacaaaca gagctattct tctcgatcgt gggacgcaat taagtactgg cttatcatca
     1501 gaagttttac ttgaatatcg tcgtttacta caccgggaag aaagtaagta tttttctcag
     1561 ttagcaaagg aagttgctgc ccacgcggac gccactcgcg atattaatca aacccccacc
     1621 gcgccggcta agatagaaat tagtgctaat gctgaaaccc ctgctgtaga ggaaacctcg
     1681 ccctctcgta cagataaact atcgttcggt gaaggtgaag cagaggttga aaatgttgaa
     1741 ttgtttacgg cttctggtga acgtagcaat gcctttaacc ctggtgataa aatcagagtt
     1801 cgtgttaatt gtaaagtaaa taaagatata aaccacatta acgttggcat tcgtattcgc
     1861 aataaagaag gcgtcaaaat ttattcatgg ggaacgttaa accaggacat gtatgttcgt
     1921 ttccatcaac ttaaagatcc ccaattttgg gaacgtgagt ttaaagcagg cgagcgtttt
     1981 tatgttgata tggaatttga atgtacttta ggaacaaacc tatatgaaat acaggcggcg
     2041 atatcctatg aggggacacc taattatttc tcgcagcgca ttttacactg gcgtgatgaa
     2101 gccgcgtttt ttcacgttaa tgtcctgcgt gatgaatatt tctttggtgg cattctggat
     2161 ctcaagatga ctgcagagtg gtaagttgat gaatgatatt tcaataactg attatctcgg
     2221 gcctggggtg tatttattac aaaattaccc aaaagaaact gagggcctga ttgcagagaa
     2281 gggttataag gtgcacaact gcgctgatct ggcgcagtgt aa
//
LOCUS       DAPHBJ010000314         2315 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.317, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000314 DAPHBJ010000000
VERSION     DAPHBJ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.317"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            72..1244
                     /gene="istA"
                     /locus_tag="RHZ76_004443"
     CDS             72..1244
                     /gene="istA"
                     /locus_tag="RHZ76_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HDV2814944.1"
                     /translation="MLSREDFYMIKQMRQQGVYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRRFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFSLEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1244..2041
                     /gene="istB"
                     /locus_tag="RHZ76_004444"
     CDS             1244..2041
                     /gene="istB"
                     /locus_tag="RHZ76_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HDV2814945.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERCENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVESGQHQ"
BASE COUNT          614 a          563 c          636 g          502 t
ORIGIN      
        1 tcaccattta gattgtccgc tccacccaac atgttgtttc cttaaggtcc cacaacaaaa
       61 aggacatcaa catgctgagc agagaggact tttacatgat aaagcaaatg cgccagcagg
      121 gcgtgtacat tgtcgatatt gcgactcaga ttggttgctc tgaacgaacg gtcagacgct
      181 acctcaaata ccctgaaccg ccagccagaa aaacccgcca caaaatggtt aagctgaaac
      241 cgtttatgga ttacatcgac atgcgcctgg cagagaatgt ctggaatagc gaggttatct
      301 tcgcggagat taaggcaatg ggctatacgg gcggacgttc catgctgcgt tactacatcc
      361 agcccaaacg aaaaatgcgt ccgtcaaaaa gaacagttcg cttcgaaact cagcctggat
      421 accagctcca gcacgactgg ggcgaagttg aggtggaggt tgccgggcaa cggtgcaaag
      481 ttaactttgc ggttaatacg ctggggttct cccgccgctt ccatgtcttc gccgcaccaa
      541 aacaggatgc tgagcatacc tacgaatcac tggttcgcgc cttccgctac ttcggtggtt
      601 gtgtgaaaac ggtgctggtt gataaccaga aggctgcggt gctgaagaat aacaacggga
      661 aagtcgtgtt caactccgga ttcctgttgc tggccgacca ctataacttc ctgccacggg
      721 catgccgtcc acgcagggcc agaacaaaag gtaaggttga gcggatggtg aaatacctca
      781 aggagaactt cttcgtccgg taccgcaggt tcgacagctt cactcatgtc aatcaacaac
      841 tggagcaatg gatagccgat gtggctgaca aacgggaact tcgccagttc aaagaaacgc
      901 cggaacagcg cttctcgctg gagcaggaac atctgcagcc gttaccggat acggacttcg
      961 ataccagtta cttcgacatc cgccatgtgt cctgggacag ctatatcgag gttggtggta
     1021 atcgttacag cgttcccgaa gcgctgtgtg gtcagccggt atcgatacga atatcgctgg
     1081 atgacgagtt gcggatctac agtaatgaga aactggtggc ctcacatcgc ctctgttcgg
     1141 catcgtctgg ctggcagaca gtgccggagc atcatgcccc gctctggcag caggtcagtc
     1201 aggtggaaca tcgaccactg agtgcctatg aggagctgtt gtgatgcatg agctggaagt
     1261 cctgctgagt cgcctgaaaa tggagcatct gagttatcac gttgaaagcc tgctggaaca
     1321 ggctgctaaa aaagagctga actaccggga gttcctgtgc atggcgctac agcaggaatg
     1381 gaacggcagg catcagcgcg gtatggagtc ccggctgaag caggctcgtc tgccgtgggt
     1441 caaaacgctg gagcagttcg actttacctt ccagccgggc atcgatcgta aggttgtccg
     1501 ggaactggct ggtctggcgt tcgtggagcg ctgcgaaaac gtgatcctgc tgggacctcc
     1561 tggtgtcgga aaaactcatc tggccatagc tcttggcgta aaagcggtgg atgcgggaca
     1621 tcgggtactg tttatgccac tggacagact gatcgcgaca ctgatgaaag cgaaacagga
     1681 aaaccggctg gagcgtcagc tacagcaact gagttatgcc cgggtgttga tcctggatga
     1741 aataggttat ctgccgatga acagagagga agccagcctg ttcttccggc tactgaaccg
     1801 tcgatatgaa aaagcgagca tcatactgac gtcaaacaaa gggttcgcag actggggaga
     1861 aatgttcgga gatcacgtgc tggcaacagc gatcctggac cggctgctac atcactcaac
     1921 cacgctgaat atcaaaggag agagttaccg gttaaaagag aaacgtaaag caggagtgct
     1981 gaccaaaaac acaacgccaa tcagtgatga tgaaatggtg gaaagcggac agcatcagta
     2041 acgaaagtat cttagcgggc atgaaaatgg caaataacgg ttaaaaatcg tggcgttgac
     2101 aaaattccat gggacacgcc cagctttcct ccatggtctg gcgattaagg gggttaaatg
     2161 ccacggtacg caaccagctt ccctccatgg tctggcgatt aagggggctc aatgccacgg
     2221 taccctacca actttctacc atggtcagtc gattcaagtg gttaaatttc gttattgaat
     2281 aagcttaaaa tggtttctta ttccaaccag aaagt
//
LOCUS       DAPHBJ010000315         2298 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.318, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000315 DAPHBJ010000000
VERSION     DAPHBJ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.318"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            9..632
                     /locus_tag="RHZ76_004445"
     CDS             9..632
                     /locus_tag="RHZ76_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDV2814946.1"
                     /translation="MNIYDSEYGSHVKSYKERIIDVITKSVKEHGRTLAIRVDLHDPV
                     MLDNGDTISCIANTDSGSISRFTNSLKAKLAADEQRKRKEGKRVHPNTLRYAWVREFT
                     QNGKRHFHVFLFLNKDAYYHLGDFNLDEDTLRTMITSAWCSALSITPEEGQHLVQYPS
                     NGKYTLNRDDILNDIYPDDLLNRIDYLTKVKSKNFDDGYRSFGCSNN"
     gene            complement(722..2266)
                     /locus_tag="RHZ76_004446"
     CDS             complement(722..2266)
                     /locus_tag="RHZ76_004446"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814947.1"
                     /translation="MAMNLGFFFGAGAEIGYGLPSGGKFAIDLFRQDPSQYKTELRQQ
                     LRLVDSRSPYANDWLPQGYADKSIYAFGRNEFSSIIESSIEYKREEIIRRLNRFDQEC
                     DDALLTLGIDKAILENLFSELTGHVIGERLYTHDIRLNELLARDVRLFESEYYSAMLD
                     VVRESDNNDDLKRYVISFLQLLVGAHGQSLVQRLNQELFEAAPDDLPIFDDITGMFRL
                     EFNRIGSTALELLLEENRNFNIDEDATALTLFCAIAQKVLENLFTTVLDYQKLIDDHF
                     RYLFSPSTEWAKFTRMVIFLKIARDYISSQAPSIEDLPDHGYYHDLATFDGDLTISAI
                     GTANYNSLLAEVFRGMNIELPQIIHLNGSVHDYYNPYKNAVITCENPDNVDQRQIHVP
                     FMLTQSGLKPLTSVTMSRRYVSLFDAYAESDAIVVVGFGFNKDDSHINGLFRELVENK
                     GRKLILISRRVDGSVEEQKRRLRNKLRISHEFNHLLIVIPVDDLTRCQGDRLWLEQVV
                     DTLNEG"
BASE COUNT          733 a          458 c          437 g          670 t
ORIGIN      
        1 gaattattat gaatatctac gatagcgagt atggcagtca tgttaaatca tacaaagaaa
       61 ggattatcga tgttattaca aaatctgtta aagaacacgg acgtacactg gcaatacggg
      121 tggatctgca tgacccggtt atgctggata atggtgatac tatttcctgt atcgctaata
      181 ctgattcagg atctatatca agatttacca attcactgaa agccaaactg gcagcagatg
      241 aacagcgcaa gcgtaaagaa ggtaaacggg tacacccgaa tacgctccgc tatgcgtggg
      301 tgagggaatt tacccagaat ggtaagcgtc attttcacgt atttctgttt ctgaacaagg
      361 atgcttacta ccacttaggg gatttcaatc ttgatgaaga tacgttaaga actatgatca
      421 cctcggcatg gtgcagcgca ttaagtataa ctcccgaaga gggacagcac ctagtacagt
      481 atccttctaa tggtaaatat accctgaatc gtgatgatat tcttaatgat atttatcccg
      541 acgatttact gaatcgaatt gattatctca ccaaagtgaa aagcaagaac tttgatgacg
      601 gttatagatc gtttgggtgt agtaataatt aactagcatt aattagggcc cttatcagag
      661 agataagggc tggcgagtat taatagaaac cataatggtt atagcaccat catcagctgc
      721 tttatccctc gttcaaagta tcaacaactt gctctaacca tagtcggtca ccttgacacc
      781 ttgtgagatc atctactgga attactatta gcaaatggtt aaactcatgc gaaatacgca
      841 gtttgtttcg caaccgccgt ttctgttctt caacacttcc atccacacgc ctcgaaataa
      901 gaattaattt ccgtccttta ttttcgacca gttcacggaa aagtccatta atatggctat
      961 catctttatt aaatccaaaa cctacaacaa caatagcatc agattcagca taagcatcaa
     1021 aaagactaac gtatcgtctt gacatagtta ctgatgttaa aggcttaaga ccgctttgcg
     1081 tcagcatgaa tggaacatga atctgacgtt ggtctacgtt atctggattt tcacaagtaa
     1141 taaccgcatt tttataaggg ttataatagt cgtgtacact gccgttcagg tgaataattt
     1201 gtggtaattc tatattcata ccacggaaaa cctctgccag aaggctgtta taattagccg
     1261 taccaatggc tgaaattgtt agatcaccat caaatgtagc taagtcatga taatagccat
     1321 gatcgggaag atcttcgata gatggtgcct gggaactaat ataatcgcgt gcgatcttga
     1381 ggaaaataac catccgagta aatttagccc attctgtgga aggtgagaag agatatcgga
     1441 aatggtcatc tatcaatttc tggtagtcga gtactgtagt gaacagattc tctaatactt
     1501 tttgggctat agcacagaaa agtgtaagag ctgttgcatc ctcatcaata ttgaaatttc
     1561 tattttcttc cagcaatagt tccaaagctg tcgaaccaat gcgattaaac tccagacgga
     1621 acataccggt aatatcatcg aagatgggta aatcatccgg tgcagcctca aataactctt
     1681 ggtttaggcg ttgaacaaga gattgtccgt gtgcaccgac caataattgt aaaaatgata
     1741 tgacgtacct ttttaaatca tcattgttat ctgattctcg tactacgtcc aacatagcag
     1801 agtaatattc agactcgaat aacctaacat ctctggctag taactcgtta agacgaatat
     1861 catgtgtata cagacgctca ccaatgacat gtcctgttaa ctctgaaaat aaattttcta
     1921 atatggcctt atcaattcct agagttaata gggcatcatc acactcttga tcaaaacgat
     1981 ttagacggcg aattatttct tcacgtttat attcgataga tgattcaata atactggaaa
     2041 actcgttacg tccaaaagca taaatgcttt tatcagcata cccctgaggt agccaatcat
     2101 tggcgtaagg tgagcgtgaa tccactaatc gcagttgttg gcggagctca gttttatact
     2161 gacttggatc ttgtctaaaa agatcaatgg caaatttgcc tccacttggt aaaccataac
     2221 caatttctgc accagcacca aaaaaaaatc ctaaattcat agccatcacc tacttattta
     2281 agttaactag tggaaccg
//
LOCUS       DAPHBJ010000316         2262 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.319, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000316 DAPHBJ010000000
VERSION     DAPHBJ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.319"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            81..584
                     /locus_tag="RHZ76_004447"
     CDS             81..584
                     /locus_tag="RHZ76_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV2814948.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(630..1046)
                     /gene="rraB"
                     /locus_tag="RHZ76_004448"
     CDS             complement(630..1046)
                     /gene="rraB"
                     /locus_tag="RHZ76_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HDV2814949.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1208..2212
                     /gene="argF"
                     /locus_tag="RHZ76_004449"
     CDS             1208..2212
                     /gene="argF"
                     /locus_tag="RHZ76_004449"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDV2814950.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNTLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
BASE COUNT          579 a          535 c          570 g          578 t
ORIGIN      
        1 ctttataaga cgcttgcgca cgtaagaaga gagtggtatt aaacaaatgt ataattactt
       61 tatttttatt gggttaaact atgaataaca ttgcgccgca atcacctgta atgcgtcgcc
      121 tgacgttgca ggataatcct gctatcgccc gcgtcattcg tcaggtatcc gccgaatacg
      181 gtcttaccgc tgataaaggc tacaccgtcg ccgatccgaa tcttgacgag ctgtatcaag
      241 tgtatagtca gcctggtcat gcatattggg tggttgagta cgagggtgaa gtggtcggcg
      301 gcggtgggat tgcgccatta gcaggtagtg aatcggacat ttgcgaactg caaaagatgt
      361 attttcttcc ggctattcgc ggtaaagggc tggcgaaaaa actggcctta atggcgatgg
      421 agcaagcacg agagatgggc ttcaaacgct gctatctgga aacgaccgct tttttaaagg
      481 aagccattgc gctttatgag catttgggct ttgagcatat cgactatgcg cttggctgca
      541 cgggccatgt cgattgtgaa gtgcggatgc tacgcgaact ctaaaaaaat gcccgctagt
      601 gtgtgctatc gggcatttta atcaattaat tagtggcgaa caccgtcatc gtcttcatcg
      661 acaaaatctt catcgtcgcc gtcttcgcca ttgggatctt caaagtaagt gccccaaccg
      721 tcgtactcaa cgtcaaattt ctccgccagc gtcatcagtt gttcaacctg ggcatcgatc
      781 agatcggcat tcaacgcgca ctcgctgagg atgtcgcagc aaatcacgat atcaccatct
      841 tcaacttcca gctcttctgg atcggtcact tcgtaaccga gtttaaacgc ttcaactgct
      901 gctttttcca gggtttccag atcgtctgcg gaaagatggt gttcgatggt gtacagcgcg
      961 tccggatcgc tgccatcttc cagtaattct tcaataatca aacgtgtttc ttcacgctgt
     1021 tcttccagtt gttccgggtt tgccatggct cattcctctt aatgtcctgc cgatactctt
     1081 attgtcacac accactgaca ttgcctccac ctttgtgaca aagatttatg ctttagactt
     1141 gcaaatgaat aatcatccat ataaattgaa ttttaattca ttgaggcatt agccacagga
     1201 gggatctatg tccgggtttt atcataagca tttcctgaaa ttactcgatt tcacgccagc
     1261 tgaactcaac accttgttgc agttagccgc gaagctgaaa gccgataaga aaagcggtaa
     1321 agaagaagct aaactcactg gtaaaaacat cgcgctcatc ttcgaaaaag actcgactcg
     1381 tacccgatgc tctttcgaag ttgccgcata tgaccagggt gctcgtgtta cttatctcgg
     1441 cccaagcggc agccagattg gtcataaaga gtcgattaaa gacactgccc gcgtgcttgg
     1501 tcgcatgtat gacggtattc agtatcgcgg ctatggtcag gagattgtcg aaacactggc
     1561 ggaatacgct ggcgtgccgg tatggaacgg cctgaccaat gagtttcacc ccacgcagct
     1621 gctggcggat ctcctcacca tgcaggagca tttgcccggt aaaacattca acgaaatgac
     1681 gctggtctat gcaggtgacg cacgtaacaa catgggcaat tcgatgctcg aagctgcggc
     1741 gcttaccggt ctggatttgc gtctggtcgc gccacaggca tgctggccgg aagctgcgct
     1801 ggttacggaa tgccgcgccc tggcacagca aaatggcggg aatattacgc tgacggaaga
     1861 tgtagccaaa ggagttgaag gtgctgactt tatctatacc gatgtatggg tgtcgatggg
     1921 ggaagcaaaa gagaaatggg cagaacggat tgcattactg cgtgattatc aggtgaacag
     1981 caagatgatg cagttgaccg gtaacccgga ggtcaaattc ctccactgcc tgcccgcgtt
     2041 tcatgacgac cagacgacgc ttggtaagaa aatggcggaa gagtttggcc tacatggcgg
     2101 aatggaagtg actgatgagg tcttcgaatc tgccgccagc attgtttttg atcaggcgga
     2161 aaaccgcatg cataccatca aagcagtgat ggtcgcgacg ctcagtaaat aattgatatc
     2221 gctataaaga tgccgggaaa tacaaaaaca tcccggcatc aa
//
LOCUS       DAPHBJ010000317         2243 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.320, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000317 DAPHBJ010000000
VERSION     DAPHBJ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.320"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..784
                     /gene="nirC"
                     /locus_tag="RHZ76_004450"
     CDS             <1..784
                     /gene="nirC"
                     /locus_tag="RHZ76_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HDV2814951.1"
                     /translation="SAANAARIARLSANNPLGFWVSSAMAGAYVGLGIILIFTLGNLL
                     DPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMWAILPQTWL
                     GNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALCNWLVCLAI
                     WMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYTLAGIGHNL
                     LWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            803..2176
                     /gene="cobA"
                     /locus_tag="RHZ76_004451"
     CDS             803..2176
                     /gene="cobA"
                     /locus_tag="RHZ76_004451"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDV2814952.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT          482 a          569 c          646 g          546 t
ORIGIN      
        1 gtctgcggct aacgctgcgc gcattgcacg cctgtcggca aataacccgc tcggattttg
       61 ggtcagctcc gccatggcgg gcgcgtatgt gggtcttggg atcatcttga ttttcacgct
      121 cggtaatttg ctcgacccgt ccgtacgtcc gctggtgatg ggcgcgacct ttggtatcgc
      181 cttaacgctg gtgattatcg ccggttctga actgttcacc ggacacacca tgttcctcac
      241 ctttggggta aaagcgggca gcatcagcca cgggcaaatg tgggcaatcc tgccgcaaac
      301 ctggctgggt aatctggtcg gttccgtctt cgttgccatg ctctatagct ggggtggcgg
      361 tagcctgctg ccggtagata ccagcatcgt tcactccgtc gcgctggcta aaaccactgc
      421 accggcaatg gtactgttct tcaaaggtgc attgtgtaac tggctggttt gcctggctat
      481 ctggatggcg ctgcgcactg aaggggcggc gaaatttatc gctatctggt ggtgtctgct
      541 ggcgtttatt gcatccggtt atgagcactc catcgctaac atgacgctgt tcgcgctctc
      601 ctggttcggc aaccacagcg aagcctacac gctggcgggt attggtcata acctgctgtg
      661 ggtgacgctg ggtaatactt tatcaggtgc cgtattcatg ggattgggtt attggtatgc
      721 tacgccgaaa gcgaatcgtc cggttgcgga caaatttaat caaactgaaa cggctgccgg
      781 ttaattacta aggggttttt acgtggatca tttgcctata ttttgccaat tacgcgatcg
      841 cgactgtctg attgtcggcg gtggtgatgt cgcggaacgc aaagcaaggt tgctgttaga
      901 cgcaggcgct cgcttaacgg tgaatgcatt agcgtttatt ccacagttca ccgcatgggc
      961 agatgcaggc atgttaaccc tcgtcgaagg gccatttgat gaaagccttc tcgacacctg
     1021 ctggctggcg attgcagcga cggatgatga cgcgcttaac cagcgcgtca gcgaagccgc
     1081 tgaagctcgt cgcatcttct gtaacgtggt cgatgcgccg aaagccgcca gctttattat
     1141 gccgtcgatt attgaccgct caccgctcat ggtagcggtc tcctctggcg gcacctctcc
     1201 ggttctggca cgcctgttgc gcgaaaaact tgaatcactg ctgccgttac atctgggcca
     1261 ggtagcgaaa tacgccgggc aattacgcgg gcgagtgaaa caacagttcg ccacgatggg
     1321 tgagcgtcgc cgtttctggg agaaattgtt cgttaacgac cgcctggcgc agtcgctggc
     1381 aaacaacgat cagaaagcca ttactgaaac caccgaacag ttaatcaacg aaccgctcga
     1441 ccatcgcggt gaagtggtgc tggttggtgc aggtccgggc gatgccgggc tgctgacact
     1501 gaaaggactg caacaaattc agcaggcaga tgtggtggtc tacgaccgtc tggtttctga
     1561 cgatattatg aatctggtac gccgcgatgc ggaccgtgtt ttcgtcggca aacgcgcggg
     1621 ataccactgc gtaccccagg aagagattaa ccagatcctg ctgcgggaag cgcaaaaagg
     1681 caaacgcgtg gtgcggctga aaggtggcga tccgtttatt tttggccgtg gtggcgaaga
     1741 gctggaaaca ctgtgcaacg cgggtattcc gttctcggtg gttccgggta ttaccgcagc
     1801 ttctggttgc tctgcctatt cgggtattcc actcacgcat cgcgattatg cccagagcgt
     1861 acgcttaatt accggacact taaaaaccgg tggcgagctg gactgggaaa acctggcggc
     1921 agaaaaacag acgctggtgt tctatatggg gttgaatcag gccgcgacta ttcagcaaaa
     1981 gctgattgaa cacggaatgc caggcgaaat gccggtggca attgtcgaaa acggtacggc
     2041 agtcacgcag cgcgtgattg acggtacgct cacacagctg ggagaactgg cgcagcaaat
     2101 gaacagtcca tcgctaatta ttattggtcg ggttgttggc ctgcgcgata aactgaactg
     2161 gttctccaac cattaattta acccgggcca gagaattctg gccttcttaa tgactctttt
     2221 tatatattca gcaaataaaa cat
//
LOCUS       DAPHBJ010000318         2216 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.321, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000318 DAPHBJ010000000
VERSION     DAPHBJ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.321"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..106)
                     /locus_tag="RHZ76_004452"
     CDS             complement(<1..106)
                     /locus_tag="RHZ76_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV2814953.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGCEQIFEDKL"
     gene            211..1173
                     /locus_tag="RHZ76_004453"
     CDS             211..1173
                     /locus_tag="RHZ76_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2814954.1"
                     /translation="MQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNNADSDTDSY
                     MWFETGDNGNEYFKWRSRQSTTTKDLMTLKWDALSVLVKALFSSEVKISTVNALRIFN
                     SSFGAIFRRSEECLHIIPTRENEGENGDIGPLRPFTLNLRTGRISMGHGLDVTGDIFA
                     NRFAINSSTGMWIHMRDQNVILGRNAVSTDGAQALLRQDHADRKFMIGGLGNKQFGIY
                     MINNSRTANGTDGQAYMDNNGNWLCGAQVIPGNYGNFDSRYVRDVRLGTRVVQTMQKG
                     VMYEKSGHAITGLGIIGAVDGDDPAVFRPIQKYINGTWYNVVQV"
     gene            1177..1704
                     /locus_tag="RHZ76_004454"
     CDS             1177..1704
                     /locus_tag="RHZ76_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDV2814955.1"
                     /translation="MQHLKNIKSGNPKTKEQYQLTKNFDVIWLWSEDGKNWYEEVKNF
                     QPDTIKIVYDANNIIVAITKDASTLNPEGYSVVEVPDITANRCADDSSKWMFRDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(1708..2139)
                     /locus_tag="RHZ76_004455"
     CDS             complement(1708..2139)
                     /locus_tag="RHZ76_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001698244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDV2814956.1"
                     /translation="MSEKLKIVYRPLQELSPYAHNARTHSTEQVAQLVESIKQFGWTN
                     PVLIDEKGEIIAGHGRVMAAEMLKMDSVPVIVLSGLTDEQKKAYRLADNRLPMNAGWD
                     EDLLRMELSDLINADFDVSLTGFGPTEIDELLTDVLPGIGP"
BASE COUNT          651 a          491 c          516 g          558 t
ORIGIN      
        1 ttaatttatc ttcaaatatt tgttcacatc ctgcacaaac aagagcgttt cgttgcaggt
       61 ctgtattctg gtcatttgtt gataccctta catagccaat cagcacgctg aatctcccgt
      121 ccaaaagcac aaatcatgcc atgcaggcca gaaaccgcca ttatctaaaa cctcggttta
      181 caggaaacgg taaacagggc caggaacgcc gtgcaaaaga atggcgatac cttgtccggt
      241 gggcttactt ttgaaaacga ctcaatcctt gcctggatta gaaatactga ctgggcaaag
      301 attggtttta aaaataatgc cgacagcgac actgattcat acatgtggtt tgaaacaggc
      361 gacaacggca atgaatattt caaatggaga agccgccaga gcaccacaac aaaagacctg
      421 atgactctta aatgggatgc tttgtctgtc cttgttaaag cccttttcag cagtgaagta
      481 aaaatatcga cagtcaatgc actgaggata tttaattcat cttttggtgc tatttttcgt
      541 cgttctgaag aatgcctgca tatcatccct acacgagaga atgagggaga aaatggtgat
      601 atagggccac tacgcccctt tacgcttaat ctcagaactg gtcggataag catggggcat
      661 ggtcttgatg ttacagggga tatatttgca aaccgttttg caattaacag tagtaccggc
      721 atgtggattc atatgcgtga ccagaatgtt attttgggac gcaatgcggt atccaccgat
      781 ggtgcgcagg cattacttcg tcaggaccac gctgatcgca aatttatgat tggtggactg
      841 gggaataagc aatttggcat ctacatgatt aataactcaa ggacagccaa tggcaccgat
      901 ggtcaggcgt acatggacaa caatggcaac tggctttgcg gtgcgcaagt tattcccggc
      961 aattatggta attttgactc acgttatgtg agagatgtcc gacttggtac acgtgttgtt
     1021 cagactatgc aaaaaggcgt gatgtatgag aaatcaggtc atgcaattac ggggcttggc
     1081 attatcggtg cagttgatgg cgatgatccg gcagtattca gaccaataca aaaatacatc
     1141 aatggcacat ggtataacgt cgtacaggtg taatttatgc agcatttaaa aaatattaag
     1201 tctggaaatc caaaaacaaa agaacaatat cagctaacaa agaattttga tgttatctgg
     1261 ttatggtccg aagacggaaa aaactggtat gaggaagtga aaaactttca gccagacaca
     1321 ataaagattg tttacgatgc aaataatatt attgtcgcca tcaccaaaga tgcctccacg
     1381 cttaaccctg aaggttatag cgtcgttgag gttccagata ttacagccaa ccgctgcgct
     1441 gatgattcca gtaagtggat gtttagggac ggagctgtgg ttaaacggat ttatacggca
     1501 gacgagcaac aacaacaggc cgaatcacaa aaggccgcat tgctttccga agctgaatca
     1561 gtcatccaac cgctggaacg cgctgtcagg ctgaatatgg caacagacga ggaacgcaca
     1621 cgactggaag catgggaacg ctacagtgtt ctggtcagcc gtgtggatac ggcaaatcct
     1681 gaatggccac aaaaaccaga gtaaaaatta aggcccgata ccgggcaaaa catccgtcaa
     1741 cagttcatca atttctgtcg ggccgaagcc tgtcagggag acatcaaaat cagcattgat
     1801 taggtccgac agctccatcc gcaacagatc ttcatcccag ccagcattca tcggtaggcg
     1861 attatctgcc aggcggtacg cctttttctg ctcatccgtc aggccagaca gaacaatgac
     1921 cggaacagaa tccattttga gcatttcagc cgccataaca cgaccgtgac ccgcaataat
     1981 ttcgcccttt tcgtcaatca gcaccggatt agtccagccg aattgcttaa tactttctac
     2041 cagttgtgcc acctgctcag tactgtgcgt cctggcgttg tgcgcatacg gtgacaattc
     2101 ttgtaatggg cgatagacta tctttaattt ctcgctcata cagccttgct ttatgaataa
     2161 aacgcacccc agcagccagt gctactgggg gcggaggtgt tgctggtaaa gttagg
//
LOCUS       DAPHBJ010000319         2196 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.322, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000319 DAPHBJ010000000
VERSION     DAPHBJ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.322"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            113..292
                     /locus_tag="RHZ76_004456"
     CDS             113..292
                     /locus_tag="RHZ76_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDV2814957.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            378..878
                     /locus_tag="RHZ76_004457"
     CDS             378..878
                     /locus_tag="RHZ76_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDV2814958.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLAINNVNK
                     KAENKA"
     gene            924..1430
                     /locus_tag="RHZ76_004458"
     CDS             924..1430
                     /locus_tag="RHZ76_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDV2814959.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPTIEAILNEEKQMADWLIQNIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(1490..2128)
                     /gene="ompW"
                     /locus_tag="RHZ76_004459"
     CDS             complement(1490..2128)
                     /gene="ompW"
                     /locus_tag="RHZ76_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HDV2814960.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
BASE COUNT          688 a          486 c          482 g          540 t
ORIGIN      
        1 tatcttctcg ttgcgcttaa tttgactaat tctcattagc gactaatttt aatgagtgtc
       61 gacacacaac actcatatta atgaaacaat gcaacgcaac gggagaaata acatggccga
      121 acatcgtggt ggttcaggaa atttcgccga agaccgtgag aaggcatccg acgcaggccg
      181 taaaggcggt cagcatagcg gcggtaattt taaaaatgat ccgcaacgcg catctgaagc
      241 gggtaaaaaa ggcggtcaac aaagcggtgg taataaatca ggcaaatcct gattcaccgt
      301 taattattaa tatcagctga taaagataat tgcatctgcg ggccgtatgg ctcgcagtga
      361 tttcactgga gaaaaatatg aatatgaaga ccattgaaga tgtatttatt cacctgcttt
      421 cagataccta cagcgcagaa aaacaattaa cccgggcact ggcaaaactc gcaagagcaa
      481 catcaaatga aaaattaagt caggcttttc atgcgcacct cgaggaaact catggacaga
      541 ttgaacgtat tgatcaagtt gtggagtccg aatcgaacct gaaaattaag cgcatgaaat
      601 gtgtggcaat ggaaggtctt attgaagaag ctaatgaggt catcgaaagt accgagaaaa
      661 acgaagtgcg tgatgccgca ctgattgccg cagcacagaa agtcgagcat tatgagattg
      721 ccagttacgg gacattagcg acgctggctg aacaattagg ttaccgtaaa gcagcgaagc
      781 ttctgaaaga aaccctggaa gaagaaaagg ccaccgacat caaactgact gatctggcca
      841 ttaataacgt aaataagaaa gccgaaaata aagcctgaaa tatgaatttt aacttttagt
      901 cattttataa agaggacatt ttcatgaatc gtattgaaca ttatcatgac tggttacgtg
      961 acgcccacgc aatggaaaag caagccgaat ctatgcttga atccatggcc agccgtatag
     1021 ataattatcc tgaactacgc gctcgtattg aacaacatct tagtgaaacc aaaaaccaga
     1081 ttgttcaact ggaaactatt cttgatcgta atgacatttc acgttcagtc attaaagatt
     1141 ccatgagtaa aatggctgcg cttgggcagt caatcggtgg tatattccct tctgatgaaa
     1201 tagtcaaagg ctctattagc ggatatgtct tcgagcaatt tgaaatcgcc tgttacacct
     1261 cactattagc agcagcaaaa aatgccggtg atacagcttc aattccaacc atcgaagcga
     1321 ttttaaatga ggaaaagcaa atggccgact ggctgattca gaatattccg caaacaactg
     1381 agaaattttt aattcgctct gaaactgatg gcgtagaagc gaagaaataa taccctttat
     1441 accatgtcct tattgacccc gtatattacg gggtcgtttt tgtgcggaat taaaaacgat
     1501 atcctgctga gaacataaac acccacggat cgaggcgtac gctatcgtgt tgctgtgcac
     1561 cgcccagctt ataattggcg gtggtatcga tatccatgta ccacactgac atgttaacca
     1621 accagtcacg gttaatcaga taatcaaccc ccacctgccc ggcagctccc caggaatctt
     1681 tcagactgag atcggaaagc cctgcctctt tgccatgatc gttaaatcca ttatcaaaga
     1741 aggtggtgta gttaatacct gccccaacgt aaggacggaa tttgctgctg gcatcaccaa
     1801 aataccactg cgccatcagt gttggtggca gatgatgaac ggttgcaata tcgccggtcg
     1861 cccgggtgcc gattttatgg cggaacggcg tcgctgccag taattccaca ccaatgttgt
     1921 cggtcgccat ataagtaaac gtaaggccca gttgcgtgtt attggtcacg ctgaatccac
     1981 ccagacttcc taacgtacca ccagcacctt ctgttggacg tacggttgca gaacctgcac
     2041 gcataaaaaa ttcgcctgct tcatgcgcaa aggcactgcc agagagaaga gttgttactg
     2101 ccaaagccgc cactgttaac tttttcatat ccgctccgtc gttatggtta taaaaacgtg
     2161 acgaatatac ctacattgga gtaacaaatg atccta
//
LOCUS       DAPHBJ010000320         2194 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.323, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000320 DAPHBJ010000000
VERSION     DAPHBJ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.323"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            300..1553
                     /locus_tag="RHZ76_004460"
     CDS             300..1553
                     /locus_tag="RHZ76_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDV2814961.1"
                     /translation="MNLIDKIALVGQRMKSEQISLKESLLVSSRVSVSDDSVDGVDRL
                     IYNHCLNKKNLSDFFGKSRVTFNKILADLEEKELVGAPIYQNKNHLYTRWDVQKIMDA
                     LNYPRYSDYYCSRTIVTQNHKGGTGKSTTSGVLAVAAALDLHLNARILLIEWDPQGSI
                     GSGMIQSVAEDDVFLTAIDAILGVYEEDSDYRKYLDLGYSEEQIIEGMPFSTHLPNLD
                     VITAFPTDARFKDKYWQCSREERTELLLRFKEVILPVLKSKYDLIIIDTPPEDSPITW
                     AADEAADGILVAVSPREYDYASTTDFMLTISERFKQSPSKGENLSWFKVLAVNVDDKS
                     PYEKIVLDKLVRTVQELFMSANIKNSEAFKAAASRGRTVLDIKKSEELCSPKQLDVAE
                     ESVMAVYQQFINEIKSFSVKQGGNV"
     gene            1550..>2194
                     /locus_tag="RHZ76_004461"
     CDS             1550..>2194
                     /locus_tag="RHZ76_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007372313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDV2814962.1"
                     /translation="MSDEQHIGNDKSRYINAPKRTEVIHRSGLQGLKGQPRLKKLFTL
                     HNGRKLEAEHIIVPAEKVELETTVHPLNPRNQEALSVNAVRDILKDIEARGVDTEGVA
                     VKRNGVYLLIEGSRRRFCCIQSAKELPLWVLPDDVNADDINSIISATQTSRRFSYREV
                     GLKYLREMEEHGFVTNEELANYHGISHVSVSKRVQAAKINSTLIALFPDYEAIPN"
BASE COUNT          671 a          421 c          511 g          591 t
ORIGIN      
        1 aatgctggtc gggtaccgtg ggatttaccc cccttattgc cacatctatg gagtgatcct
       61 gctatgtcgg aagattggta ttgttgatac ttaagaggct tttggaagac ttcaatgaac
      121 tgccaattga aattcaataa tgcagatatt ccaaaatcta agaaaagaat acagaacgtt
      181 agcgtaataa tagaattaag gttactatta aacgcataac gctaaagtat acgcattatg
      241 cgtaaagttt acgtatatac gtttaatttc actatttagg taaaagggat cggaagagaa
      301 tgaatctaat tgataagatc gcccttgtcg gtcaacgcat gaagagcgag cagatctctc
      361 tgaaagagtc attattggtc tcttcgaggg tttcggtatc ggatgatagt gtagacggtg
      421 ttgatcgcct aatctataac cactgcttga ataaaaaaaa tctgtcagat ttttttggta
      481 aatctcgcgt aacttttaac aaaatcttag cggatcttga agaaaaagaa ctggttggtg
      541 ctcctatcta ccagaacaaa aaccatctct atacccgctg ggatgtccag aaaataatgg
      601 atgctctgaa ctatcctcga tatagcgact actactgtag ccgtacaatc gtaacgcaga
      661 accataaggg tggtacgggg aagagtacaa cttcaggcgt tcttgcagtt gcggcagcac
      721 ttgatttgca tctaaacgcg cggatactgt tgatcgaatg ggacccacaa gggtctattg
      781 gaagcgggat gatccagagc gtagcggaag atgatgtttt cctgactgct attgacgcca
      841 tcttaggggt ttacgaagaa gactcagatt acagaaaata tcttgattta gggtactcgg
      901 aagaacaaat tattgaaggt atgccttttt caacacacct tccgaatctt gatgttatta
      961 ccgcattccc tacagatgca cgtttcaaag ataaatactg gcagtgttcc agagaagaac
     1021 gaactgagtt attactacgt tttaaagagg taatcctgcc ggtcttgaag agcaagtacg
     1081 acctgatcat tatcgatact ccaccagaag attcgccaat cacatgggca gcggatgaag
     1141 ctgctgatgg cattttggtt gccgtttcac ctcgtgagta tgattatgcc tcaacaactg
     1201 atttcatgtt gacgatcagt gagcgattca aacaatcgcc gagcaagggc gaaaatctaa
     1261 gctggttcaa agtgttggca gtgaatgtcg acgataagag tccctatgaa aagattgtcc
     1321 tggataaact ggtaaggaca gtgcaggaac tctttatgtc tgcaaatatc aaaaactcag
     1381 aagcgtttaa agccgcagct tctaggggta gaaccgtatt ggatatcaaa aaatcagaag
     1441 agctctgctc tcccaaacag cttgatgttg ccgaagaatc cgtgatggcg gtctaccagc
     1501 aatttattaa tgagattaag agtttttcag tgaagcaagg gggtaatgta tgagtgatga
     1561 gcagcatatc gggaacgaca aatcccgtta tattaatgcg cctaagcgta ctgaagtaat
     1621 ccatcgctcc gggcttcaag gcctgaaagg tcagccacgc ctgaagaaac tttttacatt
     1681 acacaatggg cggaagcttg aggcggaaca catcattgtt ccggcggaaa aggtggaact
     1741 ggaaaccaca gtacaccctc tgaacccacg aaatcaggag gctctttctg taaatgcagt
     1801 aagagatatt ctgaaggaca ttgaagcaag aggtgttgat actgagggcg ttgcagttaa
     1861 gcggaacgga gtgtatctgc taatagaagg tagccgcagg cgtttctgtt gcattcaatc
     1921 agcaaaagaa ttgccattat gggttttgcc tgatgatgtt aacgcagatg atattaattc
     1981 catcatatca gcaacacaaa cttctcgacg cttttcatat cgtgaagtag gtttaaagta
     2041 tttaagggaa atggaagagc atggatttgt taccaatgaa gagcttgcaa actatcatgg
     2101 tatcagtcac gtgtccgtct ctaagcgagt tcaggctgca aagattaaca gcactcttat
     2161 tgcactgttt cctgactatg aagctattcc gaat
//
LOCUS       DAPHBJ010000321         2187 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.324, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000321 DAPHBJ010000000
VERSION     DAPHBJ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.324"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(76..873)
                     /gene="mtfA"
                     /locus_tag="RHZ76_004462"
     CDS             complement(76..873)
                     /gene="mtfA"
                     /locus_tag="RHZ76_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HDV2814963.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLLRLHHANDTDSFSATNVH"
     gene            967..1056
                     /locus_tag="RHZ76_004463"
     tRNA            967..1056
                     /locus_tag="RHZ76_004463"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1001..1003,aa:Ser,seq:cga)
     gene            complement(1626..2156)
                     /locus_tag="RHZ76_004464"
     CDS             complement(1626..2156)
                     /locus_tag="RHZ76_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HDV2814964.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
BASE COUNT          632 a          471 c          480 g          604 t
ORIGIN      
        1 ttagcggtac cttgtctgtc aacactaaat ttcataagtc atttcaattg gttaattaat
       61 ctgcaaagtt gttaattaat gaacattcgt cgccgaaaac gagtctgtat cattagcgtg
      121 atgcagtctc agcaaaggat cttgttgata aaattggcag aaacgttgcc acaatgaagg
      181 gaaacgagga gcaaaaagtt ctggggcgct aaagaaatat tcagaaagta cggcaaaaca
      241 ttcagcagga tcactggcag cataagcatc aatgctcgcc gcattctcgc caaccaattc
      301 gatttcttcc tgaatgttgt tcattgcagc atgaagatcg tgttcccagc cagcaacctc
      361 acgcaacgga ataaagggaa ctccgctggc gcgatcgccg ttacgggtgt ccagcttatg
      421 agcgacttca tgaataatca ggttaaaacc agaagcatca aaagaatctt gtatatccaa
      481 ccagttcaaa acgataggcc cttgctgcca gctctgacct gactgaacaa tacgttggtt
      541 atgcaccaga ccgatatcgt cttcccattc atcatcgacc acaaatggcg caggataaat
      601 taagacttca tgaaaaccat ccagccattc cagtcctaac tccagaacgg gtaggcaaaa
      661 tagaagtgct atccggcagc ttcttaatga atccagttca aagccctgta aaggaacaag
      721 ccgcttttgc tgtaaaaaac gttcggcaag agtgactaat ttgctttgtt cctgttccgt
      781 cagacccgtt aaaaggggga tcgatagtgc ttcctgccag ggaagggcag tttgatgtgc
      841 tgattcttgt actttccagg gccacttaat catcgttttg ctcgcaaact cgtcacttga
      901 acaaaattgc acggacgggg actgttaaaa tgccaaattt cctggcatca tggcaaccat
      961 ctgaacggag agatgccgga gcggctgaac ggaccggtct cgaaaaccgg agtaggggca
     1021 actctaccgg gggttcaaat ccccctctct ccgccacttt atcaatgact tatctcccga
     1081 cttcccgcct tgcttttcct aaacagaaca atcgtagaat attcttgaag ggttagatcg
     1141 tcactgtttt ctgttcgata ctgtgacatt cagcacttga ttcgctatgg atctgacagg
     1201 aaagtttcga gcgaatatgt gcagttattc agtcgttttc ttatcggtca ccattcttct
     1261 tttagacatt catcctatga aactgctgta aagttggtgg tgggaactga agttgcgtag
     1321 agaaggggtc aatatttgag ccaaaaaagg aacgcggccg tcagattaaa gccgcgggac
     1381 aaagtccata atttccaaga gttgttgaat ttcccagctt ttttgaaagc ctttgtatca
     1441 tactaacaag taaaaaaggt ttaaatacca aaattaacct gtatttttga taagtcttac
     1501 cattgtacga tttacacagt ttctgctcct ccttgttgat agtggaaagg gtgcattgtg
     1561 cacttgcgta aacatttatg acgcgttgat gtccatggtt cacatttcct tgtcagggga
     1621 tcgacttatt tgcggcgagg catcattcgt aacagagtat tatctttctg aatgtagtga
     1681 tgaaagagtg ctgcgccagc gtgagctgcg attaaaaagt aacctatatt tgcccaggtt
     1741 tcgtgaatat tttttatcag tgctttaatt tcgctgtttg gggtaacaaa gggagacaca
     1801 ttgaaaccaa ggaaactcca cgattttcca ctgtaagcca tcaaagcaat ccccagcaga
     1861 ggaagtgcaa ggaaggttat ataaagcatg atgtgcatta gcgaagccgc tttcatttgc
     1921 caggcaggtg gcggtggtac aatagaagga tcactataac ggtgttttat aatcaggcgt
     1981 gaaaacatta acacccaaac gaatataccc gcattgtaat gtgtttctcg catcagcaga
     2041 taagtactac tacctttagg aaaccagcca cggagttcca tcgcggcata ggttattgca
     2101 acgaaaagca gcgttatcca atgtaaataa atttgagttt ttgagaatcg gttcattttt
     2161 gtagctcctg taattttcac agtcaag
//
LOCUS       DAPHBJ010000322         2174 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.325, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000322 DAPHBJ010000000
VERSION     DAPHBJ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.325"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..499)
                     /locus_tag="RHZ76_004465"
     CDS             complement(<1..499)
                     /locus_tag="RHZ76_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
                     /protein_id="HDV2814965.1"
                     /translation="MFGLRKVNRNTHTFETTFQNWKENGEDVALLMPEFSAKWLPIAE
                     ESGSWSGWVLLGEIFPLISSELAGMALMPETERLIGEWLSLSSSPLNLKHPELKYNRL
                     RVGKVFDGVLSPAQPLIRIWTGELNLWLDKVTVCQYGNAPALDKKSLYCSIHFVIGFS
                     KTCYRS"
     gene            complement(499..618)
                     /locus_tag="RHZ76_004466"
                     /pseudo
     CDS             complement(499..618)
                     /locus_tag="RHZ76_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314987.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            complement(631..>2174)
                     /locus_tag="RHZ76_004467"
     CDS             complement(631..>2174)
                     /locus_tag="RHZ76_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814966.1"
                     /translation="GAEVTSESVFNILNSTGAATDKSYLSLNPDKYPKYRLLIHSAKL
                     QNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGTFILASTFEDDKTAAETHYDAVWL
                     RDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPDQIKRMQDVISNPKRLDGIPVQMN
                     AVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGLYLDLLIQANNTGTINAEDWEKGD
                     RLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTSSVALVTSGLERLSNLLSKKDSVF
                     VSDLLREAKVNELDETLSTTRLNHLIDKGYERITLQLDLGGESPGYLEKDKHYREADA
                     ALLNVIYPANLSKINTRRKEQVLKIVKKLAGPYGIKRYEKDNYQSANFWFNDIKTDTD
                     QNSHTKREKSFIPSTEAEWFFDSWYAKSAAIVYKESRKEEYLNDSVQFMNRSLAQITG
                     ENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSPIIPLNWSKASMTLMLKEMSNLIN
                     DEGIK"
BASE COUNT          560 a          475 c          390 g          749 t
ORIGIN      
        1 gacttctgta acatgtttta ctaaatccaa ttacaaaatg aatggagcaa tataacgatt
       61 ttttgtctaa cgctggagcg tttccgtatt ggcagactgt gactttatct aaccaaagat
      121 tcaattcccc tgtccatatt cttattagtg gttgagcagg gctcaatact ccatcaaata
      181 ctttacctac gcgcaaacga ttatatttta gttcaggatg ctttagattt aacggggaac
      241 tggataaact gagccattcc cctattaacc tttccgtttc tggcattaaa gccatcccag
      301 cgagctcaga tgaaattaag ggaaatattt ctccaagcaa tacccatcca gaccagctgc
      361 cactctcttc tgctatcggt agccatttag cactaaattc gggcattaat aatgcaacat
      421 cttcgccatt ttctttccaa ttttgaaaag tggtttcaaa cgtatgagtg tttcgattta
      481 ctttcctcag accgaacatt aactttcatc cttctgatcg ttttcgaatt tgctttcatt
      541 ttcttgctcc tcataaggct gatgtcgttg tccttgtcgt tcatcttgct cagtcaatac
      601 ccaacgttgt gctgtaactc attatgaaaa ctatttaatt ccctcatcat tgataagatt
      661 ggacatctct ttcaacatga gagtcattga agctttcgac caatttagcg gaatgatagg
      721 gcttggcgct tcatgcaaag ttcccgactt gtggatatag ttatagcttt cgggaagtgc
      781 catttctggt acgctcctgc catttgcacc aatcatattc tctcctgtta tttgggccaa
      841 tgatctattc ataaattgca ctgagtcatt caaatactct tcttttcttg attctttata
      901 aacaattgct gctgattttg cataccatga gtcaaaaaac cattctgctt ctgttgatgg
      961 aatgaagctc ttttctcttt ttgtatgcga attttgatcg gtatcggttt taatatcatt
     1021 gaaccaaaaa tttgccgact gataattatc cttctcgtat ctttttatac catacggacc
     1081 tgctagtttt ttaactattt ttagaacttg ctcttttctt ctggtgttta tctttgaaag
     1141 atttgcagga taaataacat taagtaaagc cgcatcggct tctctgtaat gtttatcttt
     1201 ttccagataa cctggggatt ctccccctaa atcaagctgt aacgttattc tttcataacc
     1261 tttatctatg aggtggttca acctcgttgt tgaaagagtt tcatccagtt cattgacttt
     1321 agcttcgcgt agtaaatcag agacaaaaac agagtctttc ttcgagagta ggtttgacaa
     1381 acgttccagt ccagaggtca ccagtgcaac ggaagatgtg tttaatcggg catcttcttc
     1441 ccatgctccg gaatcttcca taacatagaa attggcttta tccagataag caatcagcag
     1501 cgcaaccgac tttaatcggt cacctttttc ccaatcttca gcgttaatag taccagtgtt
     1561 gtttgcttgt ataaggagat caaggtaaag acctaaagca tcattttgtt tatggttcca
     1621 cagctgcggc ttgccttctt cctgcacatc tgccatcacg ggggagttgc tgtcaaatcg
     1681 gatatgcaca gcattcattt gcacgggaat tccatccagt cgttttgggt tgctaatgac
     1741 gtcctgcatc cgcttaatct ggtcgggcgt agacatataa tcccacaggg tgagcagaac
     1801 cttttttgcg gctacactat ttccctgatc tgatactaat gccatatagc cccataaact
     1861 atccctcaac cagacagcat catagtgggt ttctgccgct gttttgtcat cttcgaaagt
     1921 agaagcaaga ataaacgttc cccagggttt ctccgtcaac gttaactttc gtgtgttttc
     1981 tgttaatgtt agcaatcctt gaatttcgtc tttggtatag tggctcttta tttcattttg
     2041 taatttggct gagtgaatca aaagacgata tttaggatac ttatctggat ttaacgataa
     2101 ataactttta tctgttgctg cccccgtgga attaagaatg ttaaatacag attcactcgt
     2161 cacttctgcc cctg
//
LOCUS       DAPHBJ010000323         2171 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.326, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000323 DAPHBJ010000000
VERSION     DAPHBJ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.326"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            64..396
                     /gene="hdeA"
                     /locus_tag="RHZ76_004468"
     CDS             64..396
                     /gene="hdeA"
                     /locus_tag="RHZ76_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HDV2814967.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            512..838
                     /gene="hdeB"
                     /locus_tag="RHZ76_004469"
     CDS             512..838
                     /gene="hdeB"
                     /locus_tag="RHZ76_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HDV2814968.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            902..1549
                     /locus_tag="RHZ76_004470"
     CDS             902..1549
                     /locus_tag="RHZ76_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDV2814969.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1591..2121)
                     /locus_tag="RHZ76_004471"
     CDS             complement(1591..2121)
                     /locus_tag="RHZ76_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2814970.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
BASE COUNT          639 a          422 c          492 g          618 t
ORIGIN      
        1 tataacctca gtgtcgaaat tgattcgtga cggctctttc actttatagt tgaggatatt
       61 acgatgaaaa aagtattagg cgttattctt ggtggtctgc ttcttctgcc agttgtgagc
      121 aatgcagcgg atgcgcaaaa agcagctgat aacaaaaaac cggtcaactc ctggacctgt
      181 gaagatttcc tggctgtgga cgaatccttc cagccaactg cagttggttt tgctgaagcg
      241 ctgaacaaca aagataaacc agaagatgcg gttttagatg ttcagggtat tgcaaccgta
      301 accccagcta tcgttcaggc ttgtactcag gataaacaag ccaactttaa agataaagtt
      361 aaaggcgaat gggacaaaat taagaaagat atgtaattcc gggaatgcgt tacatcgtac
      421 ttccttgcat attgaacagg ccggaatatc ttctttaaaa gcagctattc ctcctgttca
      481 tatataatct ctatattgaa tgggttacaa aatgaatatt tcatctctcc gtaaagcgtt
      541 tatttttatg ggcgctgtag cggctttgtc actggtgaac gcacaatctg cgctggcagc
      601 caatgaatcc gctaaagata tgacctgcca ggaatttatt gatctgaatc caaaagcaat
      661 gaccccggtt gcatggtgga tgctgcatga agaaacagta tataaaggtg gcgataccgt
      721 tactttaaat gaaaccgatc tcactcaaat tcctaaagtg atcgaatact gtaagaaaaa
      781 cccgcagaaa aatttgtata ccttcaaaaa tcaagcatct aatgacttgc cgaattaatg
      841 aggtacaagt aaaaaggagt agcaagttga gccatcttgc tgctcctttt tgcattttta
      901 tatgacagca gaatttatta tacgtcttat actcgcggca attgcctgtg gcgctattgg
      961 catggaaagg caaatgcgcg gcaaaggagc agggttacgc acacatgtat taattggcat
     1021 gggaagcgcc ctgtttatga ttgtttcgaa atatggtttt gctgatgtgc tgtctttaga
     1081 tcacgtcgga ctcgacccca gccgtattgc tgctcaggtg gtgacgggcg tcgggtttat
     1141 cggtgcaggt aacattctcg ttcgtaacca aaatattgta ggtctgacga cggcagcgga
     1201 tatctgggtg accgccgcca taggtatggt tattggcagc ggtatgtacg aactgggtat
     1261 ttatggttca gtgatgacct tgttggtgct ggaagtcttc catcaattaa ccttccgcct
     1321 gatgaacaaa aattaccatt tacagctgac attagtgaac ggtaatacgg tatcaatgct
     1381 cgactggttt aagcagcaaa aaataaaaac tgatttagtt tcgttgcagg aaaatgaaga
     1441 tcatgaagtg gtcgccattg atatccagct tcatgccaca acgtcgatag aagacctgtt
     1501 gcgattatta aaaggtatgg caggggtgaa aggcgtttca attagctaat ctgtgtggca
     1561 gcgtagccag actcaccgta agcctgaaat tcacaccaga taatcaatat gctgatggcg
     1621 taccagctca ttgatccgct taagattgag gatcatcatg atattgtgtt tatggcagta
     1681 gaacgttttt agcgatattt tcagtttctc cagaatatct tccgttgtca tttgattcgc
     1741 aatgcagctc agaacctgat gttgcttgcg cgaaagtgac acagccgctt caggcacaga
     1801 gcagtaactg ccattgatga gtgcgacaaa atcaatgata gtgatagtcc gcggaacaaa
     1861 ggtcaccggc actttcccca ggcagcgttt aatattaaag ggggagaaaa taatgacatg
     1921 gacaggtttt aatcgttcaa tttgagtgag aaaattagaa tgaaatacat tattcattaa
     1981 tgtatcaata atgacgaagg cattctgatg caacattacg tcgatctctt cttcgttctg
     2041 tatatgaacg acattacctt tactcagaat gtttttcatc gcggtgaaga acatcgtatc
     2101 cctggtaatt ataagaaaca taaccgactc ctggtgcgga cattaacttt ataatcagtt
     2161 tgccaaaggt a
//
LOCUS       DAPHBJ010000324         2164 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.327, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000324 DAPHBJ010000000
VERSION     DAPHBJ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.327"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..282)
                     /gene="cas2"
                     /locus_tag="RHZ76_004472"
     CDS             complement(<1..282)
                     /gene="cas2"
                     /locus_tag="RHZ76_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HDV2814971.1"
                     /translation="MSMLVVVTENVPPRLRGRLAIWLLEVRAGVYVGDVSAKIREMIW
                     EQIAGLAEEGNVVMAWATNTESGFEFQTFGVNRRTPVDLDGLRLVSFLPV"
     gene            complement(284..1201)
                     /gene="cas1e"
                     /locus_tag="RHZ76_004473"
     CDS             complement(284..1201)
                     /gene="cas1e"
                     /locus_tag="RHZ76_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HDV2814972.1"
                     /translation="MTWLPLNPIPLKDRVSMIFLQYGQIDVIDGAFVLIDKTGSRTHI
                     PVGSVACIMLEPGTRVSHAAVRLAAQVGTLLVWVGEAGVRVYASGQPGGARSDKLLYQ
                     AKLALDEDLRLKVVRKMFELRFGEPAPARRSVEQLRGIEGSRVRATYALLAKQYGVTW
                     NGRRYDPKDWEKGDTVNQCISAATSCLYGVTEAAILAAGYAPAIGFVHTGKPLSFVYD
                     IADIIKFDTVVPKAFEIARRNPGEPDREVRLACRDIFRSSKTLAKLIPLIEDVLAAGE
                     IQPPAPPEDAQPVAIPLPVSLGDAGHRSS"
     gene            complement(1217..1816)
                     /gene="cas6e"
                     /locus_tag="RHZ76_004474"
     CDS             complement(1217..1816)
                     /gene="cas6e"
                     /locus_tag="RHZ76_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HDV2814973.1"
                     /translation="MYLSKVIIARAWSRDLYQLHQGLWHLFPNRPDAARDFLFHVEKR
                     NTPEGCHVLLQSAQMPVSTAVATVIKTKQVEFQLQVGVPLYFRLRANPIKTILDNQKR
                     LDSKGNIKRCRVPLIKEAEQIAWLQRKLGNAARVEDVHPISERPQYFSGDGKSGKIQT
                     VCFEGVITINDAPALIDLVQQGIGPAKSMGCGLLSLAPL"
     gene            complement(1803..>2164)
                     /gene="cas5e"
                     /locus_tag="RHZ76_004475"
     CDS             complement(1803..>2164)
                     /gene="cas5e"
                     /locus_tag="RHZ76_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HDV2814974.1"
                     /translation="TWREYLCDASFTVALWLTPQATMVMSELEKAVLKPRYTPYLGRR
                     SCPLTQPLFLGTCQASDPQKVLLNYEPVGGDIYSEESVDGHHLKFTVRDEPMITLPRQ
                     FASREWYVIKGGMDVSQ"
BASE COUNT          572 a          578 c          467 g          547 t
ORIGIN      
        1 aacaggtaaa aaagatacca accttaaacc atccaaatct accggggtac gcctgtttac
       61 cccaaatgtc tggaactcaa atcccgattc cgtattcgtt gcccatgcca tcactacatt
      121 gccttcttcc gccagtccag ctatttgttc ccagatcatt tcacgaattt ttgcggatac
      181 atcacctaca tatacccctg cacgtacctc caacaaccag atggctaatc tgcctcgtaa
      241 gcgcggaggt acattttcag tgaccacgac caacatactc atctcagcta ctccgatgtc
      301 cggcatctcc cagagaaacg ggaagcggaa tggcaacagg ctgtgcatct tcaggtgggg
      361 ccggcggttg tatttctcca gcagcaagca cgtcctctat aagcggaatc aatttggcta
      421 atgttttact actgcgaaaa atatccctgc acgccaaacg gacttcccgg tccggctcac
      481 cagggttacg acgcgctatc tcaaaagctt tcggtacaac agtgtcaaat ttaatgatgt
      541 ctgcaatatc gtaaacaaag gaaagaggct ttcctgtatg cacaaaccca atagcgggtg
      601 cataaccagc tgcaagtatc gccgcttcag ttacgccgta taaacaggaa gttgcagcgc
      661 taatgcattg gttgaccgta tcgccctttt cccagtcttt cggatcgtag cgacgtccat
      721 tccatgtcac gccgtattgc ttcgccagaa gtgcgtaggt tgcccgcacg cgactgcctt
      781 ctatacctcg gagttgttct acggagcgtc gggcgggcgc tggttcgcca aaccgtagtt
      841 caaacatttt gcgtaccact ttcagacgca aatcttcatc cagagcaagt tttgcctgat
      901 agagcagctt atctgaacgc gcaccaccag gctgaccaga agcataaaca cgaacacccg
      961 cttcccccac ccataccaat aatgttccaa cttgcgcagc caagcgtaca gctgcatgcg
     1021 aaacccgtgt accaggctcc agcatgatgc aggcaaccga gccaacagga atatgagtgc
     1081 ggctccctgt cttgtcgata agtacaaacg cgccatcgat tacatcgatc tgcccatatt
     1141 gcagaaagat catggagacg cgatctttga gtggaatggg gttaagagga agccaggtca
     1201 ttttattaca ccttaatcac agtggagcca gggatagcaa gccacatccc atcgatttgg
     1261 ctggcccaat accttgctgt acaagatcta ttaacgctgg cgcgtcgttg atggtgatca
     1321 ccccttcaaa gcaaaccgtt tggatctttc cacttttacc atcaccagaa aaatactgtg
     1381 gtcgttccga tatgggatgc acatcttcaa cgcgcgcggc attgcccaat ttacgctgca
     1441 accacgcgat ttgttctgct tcttttatta acggaacccg acagcgtttt atattccctt
     1501 tactgtccag gcgtttttga ttgtcgagaa tagtttttat cggatttgcc cgaagccgaa
     1561 aatagagtgg aacaccaacc tgaagttgaa attcaacctg tttagtttta atgactgtcg
     1621 caacggcagt tgaaacaggc atttgcgctg actgcaataa aacatgacag ccttctggtg
     1681 tgtttcgctt ctcaacatga aaaagaaaat cacgagcagc atccggtctg tttggaaata
     1741 aatgccataa tccctggtga agttggtaaa gatccctgct ccaggccctg gcaatgatga
     1801 ctttactgag atacatccat acctccttta atcacatacc attctcggga agcaaattgt
     1861 cgaggcaagg tgatcatcgg ttcgtcgcgc accgtaaatt ttaaatgatg cccgtcaact
     1921 gattcctcac tataaatatc gccgccaacg ggctcataat ttaatagcac cttctgagga
     1981 tccgatgcct gacatgtccc gagaaaaagc ggttgtgtta gcggacaact cctccgcccc
     2041 agatacggtg tataccgagg ctttaatact gctttttcaa gttctgacat aaccatcgtt
     2101 gcctgtggtg ttaaccagag agcgacggta aaggaggcat cacataaata ttcgcgccat
     2161 gttt
//
LOCUS       DAPHBJ010000325         2158 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.328, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000325 DAPHBJ010000000
VERSION     DAPHBJ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.328"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            207..1229
                     /locus_tag="RHZ76_004476"
     CDS             207..1229
                     /locus_tag="RHZ76_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5708 family transposase"
                     /protein_id="HDV2814975.1"
                     /translation="MTITTVGIDLAKNVFAVHCIDQNGKAVLVKPKVSRAALSELIAG
                     LPPCVIGMEACSGAHYWARLFRQYGHDVRLMAPKFVSPYRMAGKTGKNDAADAIAICE
                     AVQRPHMRFVPVKDESQQAMQCLHRTRQGFIQERTATYNRLRGLISEFGVIAPQSTDA
                     LRHVVSDQKETLPVQVRLCIDDLLTHVTNIEEKIAEYDRVLSRVAKTDHRSQQLMELK
                     GVGPTTASALVASIGNAHDFKNGRQLAAWLGLTPSQYSSGGKSKLGRITKAGDAYLRT
                     LLVQGARSVMVGAENRTDPFSRWVCSLIGRRGYWRAVVAIAAKNARLCWASLHYGDDF
                     RLYSTT"
     gene            complement(1609..>2158)
                     /locus_tag="RHZ76_004477"
     CDS             complement(1609..>2158)
                     /locus_tag="RHZ76_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDV2814976.1"
                     /translation="IFESLQRYRRLYNKSLPTVLVAEEAHTFIKRYRDDSENQDVAAV
                     CCQVFEKIAREGRKFGLGMVVSSQRPSELSPTVLSQCNTFLLHRISNDKDQEQVHKMV
                     PDNLRGLLRELPSLPSQHAILMGWASELPVLVKMKNLTKDQQPHSDDPDFWDVWTRKY
                     ADGKLVERTVDWGAVVKEWQQK"
BASE COUNT          592 a          491 c          499 g          576 t
ORIGIN      
        1 tagctaaaca tgatttcccc atcaaacagt aagtcgtgtc aattcctaga gttaacacat
       61 ttctgatcat gactcacacg ttacgttcag tcggtagcca tctgtgcaaa aaatgcgctt
      121 agcgaatgga agggacttcc cctagatatg cgcctgatta actttcgtgc aatcatagga
      181 taaacattga cccagaggga ttcgttatga ccatcaccac tgtcggtatc gatcttgcta
      241 aaaatgtgtt tgctgttcac tgcattgacc aaaatggtaa agctgttctg gttaaaccca
      301 aggtgtcgcg tgcagcactt tctgaactca tcgcaggttt acctccctgt gtcattggaa
      361 tggaagcctg ctcgggtgcc cactattggg caagattatt caggcagtat ggccatgatg
      421 tccggttgat ggctccgaaa tttgtctcac cctatcgcat ggcgggtaag accgggaaaa
      481 atgatgcagc tgatgcaata gctatttgtg aggcagtgca gcggccgcat atgcgctttg
      541 taccagtcaa agacgaaagc cagcaggcaa tgcaatgctt acatcggaca agacagggat
      601 ttatccagga aagaacagca acgtataacc gtcttcgggg cctgatatct gaattcgggg
      661 ttattgcccc acaaagcact gatgctctgc gtcatgttgt ttctgaccag aaggaaactc
      721 tgccagtaca ggttaggctg tgcattgatg atctattgac acatgtcact aatattgaag
      781 agaaaatcgc cgaatatgac agggtattat ctcgtgtggc caaaacagat catcgcagcc
      841 agcagttaat ggaactgaaa ggtgttgggc ccaccacagc gagtgcgctg gtcgccagta
      901 ttggcaatgc ccatgatttt aagaacgggc gccaactggc agcctggttg gggctaacgc
      961 catcacaata cagtagtggc ggaaaatcaa agcttggcag gataacaaaa gcgggtgatg
     1021 cctatctgcg aaccctactg gtccagggag cccgttctgt aatggtaggg gcagagaata
     1081 gaacggaccc gtttagccgt tgggtctgct cgttgattgg taggcgggga tactggcgtg
     1141 ctgtcgttgc aatcgccgcc aagaatgcga ggttgtgctg ggcatcatta cattacggcg
     1201 atgacttcag actgtattca acaacatgaa aaatttaact gagtagtaac tgaactgtaa
     1261 attgttgatg gtaaagggtt agacccccgt gagaagtatc tgcatatcct actggatatc
     1321 actatccgat taacgaagga ggatctcacg agcgtctttc atcagggtcc gaatcactag
     1381 cgattcagca tgaccgttta tagtagcgca gtcttatccc ttgcctttca ttgatgaaca
     1441 gacagaaatt aaaaaccggc gttgactgtt cggggaagcc cttatagtag gataatttcc
     1501 tttttccaag cggaccccag ggacaacgga acgcaacacc aagtagattg atgacataaa
     1561 gttccttcaa ttccgatctg ccctcccgct ttcattttct gccgcagatc acttttgttg
     1621 ccattccttt acaacagctc cccaatcgac agttctctcg accaatttcc catcagcata
     1681 ctttcttgtc caaacatccc agaagtcagg gtcatcagaa tggggttgct gatctttcgt
     1741 caaatttttc attttaacca ggacaggaag ctcagatgcc cagcccatca gtattgcatg
     1801 ttgggatggc agtgaaggca actcacggag taatcctcgc aaattatcag gtaccatttt
     1861 atgaacttgc tcctgatctt tgtcattact aattcgatgc agaagaaaag tattacattg
     1921 agataaaact gtaggtgata attctgatgg tcgttgcgag gaaaccacca tcccaagacc
     1981 aaattttctg ccttctcgtg caattttctc aaacacttgg cagcaaacag ctgcgacatc
     2041 ctgattttca gagtcatcac gataccgttt aataaatgta tgtgcttctt cagctacaag
     2101 taccgtaggt aaagatttat tatatagtct tctatagcgt tgcaatgatt caaaaata
//
LOCUS       DAPHBJ010000326         2126 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.329, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000326 DAPHBJ010000000
VERSION     DAPHBJ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.329"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..231
                     /locus_tag="RHZ76_004478"
     CDS             <1..231
                     /locus_tag="RHZ76_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814977.1"
                     /translation="ERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLKDETSVI
                     LSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            563..2086
                     /locus_tag="RHZ76_004479"
     CDS             563..2086
                     /locus_tag="RHZ76_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDV2814978.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFIFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT          574 a          449 c          470 g          633 t
ORIGIN      
        1 gagagattta tttttcaatg tgctttgttt aaaaatctcg agagggaggt gttcatgacg
       61 catgggtatg ttgatagtca tattattgat caggctttac gcttgcgttt aaaagatgaa
      121 accagtgtga ttctttctga tctctatctg caaatattgc agtacattga aatgcataag
      181 actacactaa cggatatcat tattaatgac agggaatccg tgctctctta gaacttgagt
      241 aaggttttag aataaacatc agctgtatca ccatgctgat acaaagtgag catttcaggc
      301 gttatgcttt cttattatgt ccgcaatatc aggtgtcaag aatggagagt tctcgctctc
      361 cattcttgac gcctgatatc ccgcctaact tattttgtac tgcctacatt atgctcaata
      421 attaatgggt tagatcaaga tggagaaaat tttatcttcg gcgtggatat ttcttatgct
      481 ctctaccatg ctctctgtag atgccagcgc gagcgaatga atggcatttt catgtattaa
      541 accctttggc cgggacaagc aaatgcgcaa tacactcata cccatcctcg ttgcgatatg
      601 cttgtttatt accggtgtcg ctattttgaa catccagctc tggtattccg caaaagcaga
      661 gtacctggcg ggagcaagat atgccgccaa caatatcaat catatacttg aagaagcgtc
      721 acaggcgact caaacagcgg ttaacattgc cgggaaggaa tgcgacctcg aggagcaata
      781 tcagcttggc actgaagcag ctctgaaacc tcacctgcgc acaatcatca ttctcaaaca
      841 gggaatagtc tggtgtacat ccctgcctgg gaatcgggtc ctgctgtctc gtattcctgt
      901 tgtcccggac agtaatttac tgttggctcc ggcaatcgac accgttaata gattacctat
      961 cctgctctat cagagccaat ttgcagatac gcgcattttg gttacgataa gtgatcaaca
     1021 tattcgcggg gcgcttaatg tacccttgaa aggggtaagg tatgtattac gcgtggcgga
     1081 tgacattatt ggaccaacgg gtgatgtgat gacgcttaat ggacattatc cctataccga
     1141 gaaggttcac tccacaaaat atcatttcac tattatcttt aacccgccgc cactctttat
     1201 cttctacaga cttatcgata aaggctttgg gattttaata tttattctgt taatcgcctg
     1261 cgccgctgcc ttcctgcttg atagatattt caataaaagc gcaacgcctg aagagatcct
     1321 gcgacgggca ataaataatg gggagatcgt ccctttttac caacctgtgg taaatggtcg
     1381 ggaagggaca ttgcggggag ttgaggtgtt agcccgctgg aaacaacctc acggtggata
     1441 tatatcaccc gcggcattta ttccacttgc tgaaaaatcc ggattaatcg ttccgcttac
     1501 gcaaagcctg atgaatcagg ttgccagaca gatgaacgct atcgcgagta aattgccgga
     1561 aggttttcat attggaatta attttagcgc ttcgcatatt atttcgccga cgtttgtcga
     1621 cgagtgttta aatttccgtg acagttttac ccgccgcgat ttaaaccttg ttctggaagt
     1681 caccgagcgt gagccattga atgttgatga aagtctggtt cagcggttga acattctgca
     1741 tgaaaatggt tttgtcatcg cgctggatga tttcggtact ggctactcag ggctttctta
     1801 tcttcatgac ctgcatattg attatatcaa aattgatcac agtttcgttg gccgcgtaaa
     1861 cgcagaccca gaatcaaccc gaattctgga ttgtgtattg gatctggcgc gtaaactttc
     1921 gatcagtatc gtcgctgaag gtgtcgaaac gaaagaacaa cttgactatc tgaaccaaaa
     1981 taatatcaca tttcagcagg gttattattt ctataaacct gttacataca tcgacctggt
     2041 caaaattatc ctttctaaac cgaaggtgaa ggttgtggtt gagtgaaaat tgatcaataa
     2101 ggccatggtg cggtgtaatt atagac
//
LOCUS       DAPHBJ010000327         2122 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.330, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000327 DAPHBJ010000000
VERSION     DAPHBJ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.330"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(148..855)
                     /locus_tag="RHZ76_004480"
     CDS             complement(148..855)
                     /locus_tag="RHZ76_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814979.1"
                     /translation="MKHSLFFTQDGYLLPSSVKPIYGKDGHYYAVITRECFECGGTGK
                     IANAVCVTCNGSKTSPATEEKLVLKECIEFLETIPELRCNPDLEAWKRRHSEQLALLQ
                     ADNSKFAASIAQQLNNNLFLTVRQLESFYSTYQPVSEAFSENAGFPIEHSFNIGDVVS
                     FDIIFTEVRAAFSKGSHSYFFTFTGKKQNDGFALTGNVQDHVLLGHSYHLSGKVRKIH
                     YFDSVPYALLTVFKLTA"
     gene            complement(852..1052)
                     /locus_tag="RHZ76_004481"
     CDS             complement(852..1052)
                     /locus_tag="RHZ76_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814980.1"
                     /translation="MDKIAQFTVINTYNDLLITKGTLDRNIEDIKAGKRFDHFEEGHD
                     QYVQKVTDALQLVEARLENLKK"
     gene            complement(1070..2122)
                     /locus_tag="RHZ76_004482"
     CDS             complement(1070..2122)
                     /locus_tag="RHZ76_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814981.1"
                     /translation="MEISPKALFIESGFNPREAVIGELCYEQEPVKSTIVSIKQAYKE
                     GRRVDLIKVVKKDSNYVVRQGHCRYRALSLALAEGADIQLISVVVMNYKNKSEEYLEN
                     LDGNRNNGLNPVALAYALAEALKLGSTVESLAMRYQRSSTAIRNQLKILDMPLELQRL
                     ISLNLIKKTLAIEIMLKYSNDHAKVLAHLESCSLLVPSTEPEPVLSKTSSVQTSSNDE
                     KLDNPETLPVHVIPKEVKTKSITRSSLGIKRLSHKKTERLKTEFIGLIDTVRPALNDS
                     NKLTELIFDGQHSNLFTNISSTSITKTTESVKITLDEAKLQLLLSLLNDENTAIIIPK
                     SKVTELHEIRDLLTSE"
BASE COUNT          601 a          422 c          447 g          652 t
ORIGIN      
        1 cggcttattt tcggagtgta aaaccgggta aaaacccgat gcgtgagcaa cagatttgac
       61 gtgagtgagt cggcacgaca gtgcagaaag ccggtggcta atgtaaaaac ggcctgtatt
      121 tgggagttcc ggtcactaga caccaggcta tgctgttaat ttaaacacag tcagtaatgc
      181 gtagggcact gagtcaaagt agtggatctt tctcactttt cccgaaagat gatagctatg
      241 ccccaataaa acgtgatcct gaacattacc agtcaacgcg aatccatcat tctgcttttt
      301 gcctgtaaaa gtaaaaaaat agctatgaga accttttgaa aacgcagctc gcacttctgt
      361 aaaaataata tcaaaactaa ctacatcacc aatgttgaaa gaatgttcaa ttggaaaacc
      421 ggcgttttct gaaaaagctt cagaaacagg ctggtatgtc gagtagaaag actccaactg
      481 acggactgtt aaaaagagat tgttgtttaa ctgctgtgca atgcttgctg caaacttaga
      541 attgtcagct tgcagcaatg ctaactgctc gctgtgccta cgtttccagg cttcaagatc
      601 gggattgcag cgaagttccg ggatagtttc aaggaattca atgcactctt tcagtaccag
      661 cttttcctct gtggctggag acgttttgga gccattgcat gtcacacaga cggcgttcgc
      721 aatctttcca gtccctccgc attcaaagca ctcacgggtg ataacggcgt aatagtgtcc
      781 atctttaccg tagataggct ttacggatga tggcagtaaa taaccatcct gagtaaaaaa
      841 aagagaatgt ttcatttttt aaggttctcc agtcgagcct ccacgagctg taaagcgtca
      901 gttaccttct gtacgtattg atcgtgccct tcttcaaaat gatcaaaacg ctttccagcc
      961 ttgatatctt caatgttgcg atcgagtgtg ccttttgtaa taagtagatc gttataagta
     1021 ttaattacag taaactgtgc aatcttgtcc ataaacggtc cttagcattc tactcacttg
     1081 ttaacaggtc acgaatttca tgcagttctg taaccttaga ttttggaatg atgatagcag
     1141 tattttcatc attcagaagg cttaataaaa gctggagttt tgcttcatct agagtgatct
     1201 ttacactttc cgttgtcttt gtgatggaag tgctgctgat gttcgtaaat aaattggaat
     1261 gttgtccgtc gaaaattaat tcggtgagtt tattactgtc gtttagtgct ggccgtacag
     1321 tatcaatcag cccgataaat tcagttttta agcgttcagt ttttttatga gaaagccgtt
     1381 taatacccaa tgaactgcga gtgatggatt ttgttttaac ttccttagga ataacatgaa
     1441 cagggagtgt ttccgggtta tctagtttct catcgtttga tgatgtttga acgcttgatg
     1501 tcttagataa aacaggttct ggttcagttg atggtactaa aagagaacac gattccaaat
     1561 gtgcaaggac ttttgcatgg tcattggaat acttcaacat gatctcaatc gcgagtgttt
     1621 ttttaatcag gtttagactg attaaacgtt gtagttctaa aggcatatca aggattttaa
     1681 gctggttacg aatagcagtg ctagaccgtt gatagcgcat agcaagggat tcaacggtag
     1741 agccaagctt cagcgcctct gccagtgcat aagctaaggc tacgggatta agaccgttat
     1801 tcctattccc atcaaggttc tccaggtatt cttcactttt attcttatag ttcataacaa
     1861 ctactgaaat gagctgaata tcagctcctt ccgcaagagc aagagatagt gctctgtaac
     1921 ggcaatgccc ctgccttaca acgtaattgg agtctttctt aacaacctta attaaatcga
     1981 ctcgtctgcc ctctttatag gcttgtttaa tagaaacaat ggtcgatttt actggttcct
     2041 gttcataaca aagttcacca attaccgctt cacgtggatt gaatccagat tcgataaaaa
     2101 gtgctttagg actgatctca at
//
LOCUS       DAPHBJ010000328         2111 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.331, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000328 DAPHBJ010000000
VERSION     DAPHBJ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.331"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1403)
                     /locus_tag="RHZ76_004483"
     CDS             complement(<1..1403)
                     /locus_tag="RHZ76_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HDV2814982.1"
                     /translation="MSCGRPFPVHVFPQIIRNAVYEVEQHTQAPQALIAASVLGVISL
                     ACQNRIDVCRLNNLRSPVSLFLLTLADSGERKSTVDKLLMKPLYQLEEACFEKYTQDL
                     IIWRNEETTFNIEKKALISKLKSDIRRNKDHSTTNERLKTLLSANPKAPVRFRQIFND
                     ATPAAIKDYLSGCWRSIGLMSDEAGTIFNGYALNELPFINKMWDGAMFTVERKSEPDK
                     LIKDARMTLSLMVQPDVFKKGYLERKGDTAKGIGFFARCLMCQPSSTQGYRQITSPVI
                     SSEHLPVFHKRLMEIVNESIVRNDENARQCLRFSAEAEKNWIEFYNKVESEMGLIGFL
                     SGFKDYASKMAENMARIAALLHKFNGDEGDIPLSAVEAAVEISAWYVDEYVRIFSKPQ
                     TLLLVSSEADELYSWIKDYCYRCVVPYIRKTTILQYGPNRFRNRSKANELLSTLYSQK
                     KIRAEKIGKTIFIQPVD"
     gene            complement(1534..1746)
                     /locus_tag="RHZ76_004484"
     CDS             complement(1534..1746)
                     /locus_tag="RHZ76_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDV2814983.1"
                     /translation="MNNPSTVRILRLPAVIQKTGMARATIYDWLNPKSPRYDASFPKK
                     RMLGVKSVGWVESEIEEWLSQRSKLI"
BASE COUNT          567 a          474 c          436 g          634 t
ORIGIN      
        1 atatcaacag gttgtatgaa aatggttttc cctatcttct ccgcccgtat tttcttttgc
       61 gaataaagtg tactgagaag ctcattcgct ttgctccgat tcctgaaccg gttcggacca
      121 tattgcaaga tggttgtctt cctgatatat ggcaccacac accggtagca ataatctttt
      181 atccacgaat aaagttcatc tgcttctgaa ctaaccaaca gcaacgtctg gggtttagag
      241 aaaattctca catactcatc cacataccag gcgcttatct ctactgcggc ttctacggca
      301 ctgaggggta tatccccttc atcaccatta aatttatgca gcagagcggc aatccttgcc
      361 atattttccg ccattttaga ggcgtaatcc ttaaagccag acaggaagcc aatcaacccc
      421 atttctgact cgaccttgtt ataaaattcg atccagttct tttcggcttc tgcagagaaa
      481 cgtaggcact gacgcgcatt ttcatcattt ctgacaatgc tctcgttaac gatctccatc
      541 aatcgtttat gaaataccgg tagatgctcg cttgatataa ccgggctggt aatttgtctg
      601 taaccctgtg ttgaactagg ctgacacatc aaacaccgcg cgaagaatcc gatccctttc
      661 gctgtatcgc ctttacgctc cagataccct tttttgaaca catcaggctg aaccatcaac
      721 gacagtgtca ttctggcgtc tttgattaat ttatctggct cgctttttct ttccacagta
      781 aacattgcgc catcccacat tttattgatg aaaggcagtt cattcagcgc atagccgtta
      841 aaaatggtac cagcttcatc agacataagc ccgatggacc gccaacaacc actgagatag
      901 tcttttatcg ccgctggtgt ggcatcattg aatatctgcc tgaacctgac cggtgctttc
      961 gggttcgccg acagcagcgt tttcagccgt tcattcgttg tggagtgatc tttgttacgg
     1021 cgaatatctg atttcagttt tgatatcagg gctttctttt caatgttaaa agtcgtttcc
     1081 tcattccgcc agataattaa atcttgggta tatttttcaa agcatgcttc ttccagttga
     1141 tacaatggct tcattagcag tttgtccacc gtgcttttgc gttcaccaga atccgccagt
     1201 gtcagtaaaa aaagtgatac tgggctgcgc agattattca gtcggcacac atcaatccgg
     1261 ttctggcagg caagcgaaat aacccccaac accgatgcag caatcaaagc ctggggtgcc
     1321 tgcgtatgct gttccacttc ataaaccgca tttctgatga tctgcgggaa gacatgtaca
     1381 gggaaaggac gaccacacga catagatacc tctcattcta ttaatttaat aaataattca
     1441 gcgaaatggc tattctgcta ttaatagcgg aaatagcagg atgactaccg acaatccgat
     1501 gacagcaatt gagtatgaat tcatgtagca tcctcaaata agtttgctgc gttgcgatag
     1561 ccattcttca atctctgatt caacccatcc aacagatttc actccgagca ttcgcttttt
     1621 ggggaaggaa gcatcgtaac gtggtgattt ggggttcaac caatcgtaga ttgttgctcg
     1681 tgccatacca gttttttgga taacagcagg taagcggagt atcctaacgg ttgatggatt
     1741 attcatgctt gattgcctct tattagttca ttttggttgt agaaagatta gcggcaagca
     1801 tcaatgcaaa aaatccgtgt accaaaaaaa cattttttac aacacttaac taattgatat
     1861 ttggtgtatt tatgttgtca gcgattgagt tttgcgaaga aattagtcaa aaatcggatt
     1921 ggcttgctgg gcatatggtc gaatgtgatt gggacatata tgttgatctg ctcagcgaaa
     1981 ggtatcccgt gatacagaag gaactaagcg agatgcgaga tcaattctgg aattctgata
     2041 aggtaggaac acgtgtgatt ttgtacagtg atccatctcg taaggagtat aaatattgta
     2101 ctgttgacgg a
//
LOCUS       DAPHBJ010000329         2048 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.332, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000329 DAPHBJ010000000
VERSION     DAPHBJ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.332"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..630
                     /locus_tag="RHZ76_004485"
     CDS             <1..630
                     /locus_tag="RHZ76_004485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDV2814984.1"
                     /translation="RKPLVIIGGGGHASVIVDILKRQKREIVAIISPDDITQRKVYSG
                     INVFSNDNEIFRFQPKDIRLINGIGALPDSEVRYKVNLYFEKMGYSFETIVADNAYVS
                     PFAFLEEGVQIFPGAIIQPGTHIGAHTIINTRVVIEHDVSLGAYNVISPGAIICGQCK
                     TEERVFIGAGAIVIQNIEIGSRAAIMANALVAENIHPQQKVYASRGIVR"
     gene            649..1707
                     /locus_tag="RHZ76_004486"
     CDS             649..1707
                     /locus_tag="RHZ76_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004723045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HDV2814985.1"
                     /translation="MNQQWKNVLISPDSSILEALEIINNEALRVALVVNENNTLLGVI
                     TDGDIRRGILKNLPLTAEVHQVMNKKPVTASPALSKKELNNLMSSHGILSIPIVDKGI
                     IVGLETITSIAATEKYDNPVFIMAGGFGTRLRPLTDNCPKPMLKVGDKPILETVVRSF
                     VKAGFTNLYISTHFLPDMIHQHFGDGDAFNAKITYIHEETPLGTGGALGLLPDSLSDS
                     LPLIMINGDVLTNIDFERLLSFHNNNNADATICVRKYDYQIPYGVITGNGNKIVSMVE
                     KPVHHFFVNAGIYVVSPDIFKSVPKNHRIDMPTLLEQFMSKNKEILMFPIHEYWLDIG
                     RIDDFNRAQADIHSLGLD"
     gene            1709..>2048
                     /locus_tag="RHZ76_004487"
     CDS             1709..>2048
                     /locus_tag="RHZ76_004487"
                     /inference="COORDINATES: protein motif:HMM:NF013567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV2814986.1"
                     /translation="MKKVAVIGLGNIATRHRHNLKKLFPGIIVFSMSSSERVLSELVS
                     NCDGYLANVDAIIQEQVDFVIVASPATYHLRHSEKLLAAGIPTFIEKPVTASFDDAKK
                     LHEIAERHATP"
BASE COUNT          655 a          345 c          429 g          619 t
ORIGIN      
        1 agaaaaccac ttgttattat tggtggggga gggcacgcca gcgtaattgt tgatattttg
       61 aaaagacaga agcgagagat tgttgctatt attagccctg atgatatcac tcagcgaaag
      121 gtatattcag gaattaatgt tttttcgaat gataatgaaa tatttcgttt ccaaccaaag
      181 gatattcgct taattaatgg aattggtgcc ttaccagact cggaagttag gtataaagtt
      241 aatttgtact ttgaaaaaat gggctatagt tttgaaacaa ttgtagctga taacgcatat
      301 gtgtcaccct ttgccttttt ggaagaagga gtacagatat ttcctggtgc cataattcaa
      361 ccagggacac atattggtgc ccatactatt attaataccc gtgtagtcat tgagcatgat
      421 gtgtctctcg gtgcatataa cgtaatctct cctggggcta taatatgtgg gcagtgcaaa
      481 actgaagagc gtgtattcat tggtgcaggt gcaattgtta ttcagaatat tgaaatcgga
      541 tcaagagccg caatcatggc gaacgcattg gtagcagaaa atatacatcc acaacaaaaa
      601 gtttatgcat cacgcggtat agtcagataa tgtttcagaa ggtatataat gaatcagcaa
      661 tggaaaaatg tattaatctc accagatagc tcgatacttg aagcactcga aattatcaat
      721 aacgaagcat taagagttgc gctggtagtt aatgaaaata acacgttatt aggagtcatt
      781 accgatggtg atattcgaag aggaattttg aaaaacctgc ctctgactgc agaagtacac
      841 caagttatga ataaaaaacc cgtaacggca agtccagcgc tttcaaaaaa agagctcaat
      901 aatctgatgt catcgcatgg tatactgtct ataccaatag ttgataaagg tatcattgtg
      961 gggctggaga ctattacaag tatcgctgca acggaaaaat atgataatcc tgtttttatt
     1021 atggccggag ggtttggtac tcgtttgaga ccactcactg ataattgccc taaaccaatg
     1081 cttaaagtag gtgataaacc tattttggag acagtggtaa gaagctttgt taaagcaggg
     1141 tttactaatt tatatatatc tacccatttt ctgccagata tgattcatca gcacttcggt
     1201 gatggtgatg cgtttaatgc aaaaatcacc tatatacatg aagaaactcc cttaggtacg
     1261 ggaggagcgt tgggattgct tcctgactcc ttatctgatt ctttgccact cattatgatc
     1321 aatggtgatg tgcttaccaa tattgatttt gagcggttac tatcatttca taataataac
     1381 aatgcggatg ctacaatctg cgtaagaaaa tatgactacc agatcccata tggtgttata
     1441 acaggtaatg gtaataaaat agtcagtatg gttgaaaagc cagtacatca tttctttgtt
     1501 aatgcaggaa tctatgttgt ttctcccgat atttttaaat cagtacccaa aaaccatcga
     1561 attgatatgc cgacgttact cgaacaattt atgagtaaga ataaagagat actaatgttc
     1621 ccgattcatg agtattggct tgatataggg cgaatagatg attttaatcg ggcacaggcc
     1681 gatattcatt ctttagggct ggattaaaat gaaaaaggtc gctgtaatcg ggttgggaaa
     1741 tatcgcaaca agacatcgcc acaatcttaa aaaacttttt ccaggaatta ttgttttttc
     1801 tatgtcttca agtgagcgag tgctatccga gttagtgagt aactgtgatg gttacttagc
     1861 taatgtagat gctatcatac aagagcaagt cgattttgta attgtagcat caccagcaac
     1921 ttaccattta cggcatagtg aaaaactttt ggcagcagga attcctactt ttattgaaaa
     1981 acctgttaca gcttcgtttg atgacgctaa gaagttacat gaaatagctg aaagacacgc
     2041 aactccgg
//
LOCUS       DAPHBJ010000330         2031 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.333, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000330 DAPHBJ010000000
VERSION     DAPHBJ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.333"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(133..1053)
                     /locus_tag="RHZ76_004488"
     CDS             complement(133..1053)
                     /locus_tag="RHZ76_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814987.1"
                     /translation="MKAIYKIIEAMLVKMGFEGAVIQSVNLETGVIQSRVVKADIPVR
                     TLMPHLRRYLRDCEPLWGHDVDYHPIAQYCSGAALLKAGPVTLETFRFEVSVSTAHLR
                     LVDIEPDCLNEGVNREKLEYVYYRSSNADKDLILGMVEKGHNFINHLHELLRSRLSTA
                     LPLIFAVTEKIAESSVPQVVTSFFSVDHQINAQIVVEPVPVSVADQIISADWNVCSVS
                     GADKFLNHVNRYLSGRVMKRRIYAVIEACDGGSLSLNANLGCFTSPLLVTEYKMVQSH
                     SSSLYRKAINNSLKQVNSYAPEHSDDLLSM"
     gene            complement(1131..>2031)
                     /locus_tag="RHZ76_004489"
     CDS             complement(1131..>2031)
                     /locus_tag="RHZ76_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814988.1"
                     /translation="ISRKHSVKISNVICLSGTDGKFTGICCDADVSFDFLYSYAPAYS
                     STFLDIPFPGFEDQDIADCVKCQLDVVKNKRNRSFLIDHIRFPVSSREGFTLTRGDSY
                     DVTECEYNKERLLHLTRQGRFCEDYLTFKDGLSSFFSFVNFELHEIVKEGIRLALDVL
                     NKITSDQPDRLIKDFKYHDCFGSYNVQIFSKGLPVDILETMIAPDRLLSNLSGSRQIM
                     KNASRYLKGFALSNRVYMKYGWRLADVDTSDYAKVMSDREMHARDDLKAFCSVFFKEC
                     FASGKYEYEAYLSERQHAIRNGY"
BASE COUNT          631 a          463 c          375 g          562 t
ORIGIN      
        1 gccaagttga catttaagca ttgcactaac aaagttttta tcatcacttt cattaaacat
       61 attcacttcc tctttaatga agaaaggaag caacccccac ggggaatacg cttccccaat
      121 gggtaaaatt aattacatag aaagcaggtc atctgaatgc tctggagcat acgaattaac
      181 ttgcttaagc gagttattta tagcctttcg ataaagcgat gaagagtgtg actgaaccat
      241 tttatactca gttacgagaa gaggtgatgt aaagcaacct aagttcgcat tgagggataa
      301 agatccaccg tcgcaagctt ctataacagc gtaaatacgc cgcttcatca ccctacccga
      361 cagataacga ttaacatggt tcaggaactt atccgctcct gaaacactac acacattcca
      421 gtcagcagaa attatctggt ctgcgactga aacgggtaca ggttcgacta ctatctgggc
      481 attgatttga tggtcaacac tgaaaaagct ggtgactact tgtggaactg aactctccgc
      541 aatcttttct gtaactgcga aaatcagcgg caaagccgtt gataagcgcg agcgtaaaag
      601 ttcatgcaga tgattgataa aattatgtcc tttttcaacc ataccaagta ttagatcctt
      661 gtctgcattg ctggagcggt aatatacata ctccagtttt tcacggttca caccctcatt
      721 tagacagtcc ggctcaatat ccacaagccg caggtgagca gttgacactg aaacctcaaa
      781 ccgaaaggtt tcaagagtga ccggcccagc cttcaacaac gcagctcctg agcaatattg
      841 tgctataggg tggtaatcca cgtcatgtcc ccataaaggc tcacaatcgc gcagatatcg
      901 ccttaaatgc ggcattaaag tccggacagg aatatcagcc ttaacaactc gactctgaat
      961 cacacccgtt tccagattga cactttggat cacggctcct tcaaagccca ttttcaccag
     1021 catcgcttca attattttat aaatcgcttt cattttgtag ccctcggtta agagggaatc
     1081 ctctccaagg gaagaattcc ctcagggatt agataagttg ataataagta ttaatacccg
     1141 ttacgaatcg cgtgttgtct ttcactcagg tatgcttcgt attcatattt tccggatgcg
     1201 aaacactctt taaaaaagac actgcaaaac gccttaagat catctctggc atgcatttct
     1261 ctatctgaca taactttcgc atagtcagat gtgtcaacat ctgctaaacg ccatccgtat
     1321 ttcatgtaaa cacgattact gagggcaaag cctttaagat agcgacttgc atttttcata
     1381 atctgacgac tacctgacaa gttagacaac aacctatcag gtgcaatcat cgtttcaagg
     1441 atatcaactg gcagaccctt agaaaatatt tgtacattat aactgccgaa gcaatcatga
     1501 tacttaaagt cttttataag acggtctggt tgatcagatg taatcttgtt taaaacatca
     1561 agtgccaaac gtataccttc cttcactatt tcatgtaatt cgaaattcac aaatgaaaag
     1621 aaggaactca ggccatcctt gaacgtgaga tagtcttcac aaaaacgtcc ttgtcgggtc
     1681 agatgtagca atcgttcctt attatattca cattcagtca cgtcgtaact atcaccgcgt
     1741 gtcagagtga atccttccct tgaggaaact ggaaatctaa tatggtcaat taaaaaagac
     1801 cgattccgtt tatttttaac tacatctaac tgacatttaa cacaatccgc gatgtcctgg
     1861 tcttcaaacc caggaaacgg gatatctaaa aatgttgaac tgtatgcggg ggcatacgaa
     1921 taaagaaaat caaatgaaac atcggcgtca cagcatattc cagtaaattt accgtcggtg
     1981 ccactcaagc agataacatt actgatcttc actgaatgtt tacgcgatat c
//
LOCUS       DAPHBJ010000331         2029 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.334, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000331 DAPHBJ010000000
VERSION     DAPHBJ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.334"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..1986
                     /gene="yjcS"
                     /locus_tag="RHZ76_004490"
     CDS             1..1986
                     /gene="yjcS"
                     /locus_tag="RHZ76_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HDV2814989.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKVYYRADDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     TGKNISLNFNMGNGDNLNLTLNDSVLNYRKTLQPQANASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT          524 a          544 c          526 g          435 t
ORIGIN      
        1 atgaataact ctcggttatt ccgtttgagc aggattgtta ttgcgttaac tgccgccagc
       61 ggcatgatgg taaataccgc taacgcgaaa gaggaagcga aagccgccac tcaatatacc
      121 caacaggtta atcagaatta cgccaaatca ttaccgttta gcgatcgtca ggattttgac
      181 gatgcccagc gtggatttat cgccccgctg ctggatgaag gtattctgcg tgatgcgaac
      241 ggtaaagttt actaccgcgc ggacgattac aaatttgata ttaatgccgc agcgccggaa
      301 accgtaaacc ccagcctgtg gcgtcagtcg caaatcaacg gtatttctgg cctgttcaaa
      361 gtcaccgata aaatgtatca ggtgcgcggc caggatatct ctaacattac gttcgttgag
      421 ggcgagaaag gcattattgt tatcgacccg ctggtgacgc cgcctgccgc aaaagccgca
      481 cttgaccttt acttccagca tcgtccgcaa aaaccgattg ttgccgttat ctacactcac
      541 agccacaccg accactatgg tggcgtgaaa ggcattatct ctgaagccga tgttaaatcc
      601 ggcaaagttc aggtgattgc ccctgcaggc tttatggacg aagccatcag cgaaaacgtg
      661 ctggcgggta acatcatgag ccgccgtgcg ctctactctt acggtctgtt actgccgcac
      721 aacgcgcaag gcaacgtggg taatggcctt ggcgtgacgc tggcaacggg cgacccgagc
      781 attattgcac cgactaaaac tatcgtccga actggcgaga agatgattat cgacggcctg
      841 gagtttgact tcctgatgac cccaggtagc gaagcgccag ccgaaatgca cttctatatt
      901 ccggccctga aagccctgtg taccgccgag aacgccacgc ataccctgca caacttctac
      961 actctacgcg gcgcgaaaac ccgcgacacc agcaagtgga ccgagtatct gaacgaaacg
     1021 ctggatatgt ggggtaacga cgcggaagtc ctgtttatgc cgcacacctg gccggtctgg
     1081 ggcaataagc atatcaatga ttatattggt aaatatcgcg atactatcaa gtacattcac
     1141 gaccagaccc tgcacctggc gaaccagggc tacaccatga atgaaatcgg cgacatgatt
     1201 aagctgccgc ctgcacttgc caataactgg gccagccgtg gctattacgg ttctgtcagc
     1261 cacaacgccc gcgcggtgta taacttctat cttggctatt acgacggtaa cccggctaac
     1321 ctgcatccgt atggtcaggt ggagatgggt aaacgttacg tgcaggcgct gggcggttct
     1381 gcccgtgtca tcaacctggc gcaagaagcg aacaagcaag gtgattaccg ctggtcggca
     1441 gaactgctga aacaggtgat tgccgccaac ccgggtgacc aggtcgcgaa gaatctgcaa
     1501 gcgaataact ttgaacagct gggctatcag gccgagtccg ccacctggcg cggtttctac
     1561 ctgaccggcg cgaaagagct gcgcgaaggg gtgcataagt tcagccacgg caccaccggt
     1621 tccccggaca ccattcgcgg gatgtcggtc gaaatgctgt tcgactttat gtccgttcgc
     1681 ctcgatagcg cgaaagccac gggtaaaaat atcagcctga acttcaatat gggcaacggc
     1741 gataacctca acctgacgct gaacgatagt gtgcttaact accggaaaac gctgcaaccg
     1801 caagccaacg cctctttcta catcagccgt gaagatctgc acgccgtgct gaccggacag
     1861 gcaaaaatgg cggatctggt aaaagcgaag aaagccaaaa ttattggcaa tggcgcgaaa
     1921 ctggaagaaa ttattgcctg tctggataat ttcgatttgt gggtgaatat cgtaacccca
     1981 aattaatgcc actcgctttt gggggcgctg aagcccccaa aagcattta
//
LOCUS       DAPHBJ010000332         1990 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.335, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000332 DAPHBJ010000000
VERSION     DAPHBJ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.335"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(99..1283)
                     /locus_tag="RHZ76_004491"
     CDS             complement(99..1283)
                     /locus_tag="RHZ76_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV2814990.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            complement(1583..1677)
                     /locus_tag="RHZ76_004492"
     tRNA            complement(1583..1677)
                     /locus_tag="RHZ76_004492"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1641..1643),aa:Sec,seq:tca)
BASE COUNT          548 a          438 c          430 g          574 t
ORIGIN      
        1 cgcaaaaaaa ataatataat aacaaaaatc ctccagcctt gtaggccaga ggataaaata
       61 cttaattgag gaaaatatca gggaaaagta tttctgattt aactttcccg gctggagagt
      121 gaatcaatgt aatcagcgta ccattgcatc atttcacggc ggccatcgag atacagggca
      181 tgattatagg tacccctgat ggaattttta tccacatgag ctaactgcat ttcaatccag
      241 gcggaattaa agccttgttc atgcagaatt gtactcatgg tgtgtctgaa accgtgccct
      301 gtgagtcttc ccttatatcc aagtaattca attacttggt taatgctttc cttactaatt
      361 ggtttgcggg gatcatttct accgataaag actaggggat gatgttttga gattggttca
      421 agctgcttaa atagcataat gacctgctca gagagcggca caatatgagg tcgcttcatc
      481 ttcattactt ctgccgggat ctcccacaat tttgtctcaa aatcgatatc ttcccagcgt
      541 gcaaaacgca gttcttgtgt tcgtacgccg gttaacataa tgatctgagt agctgtctta
      601 gtgattatac ttccggtata acccgccaga tcgttgagga agtggggaag ttcattagca
      661 gtaagaaagg gaaaatgtac ttttttcggt gtagctaaag cactggcaag atcgggggct
      721 gggttatagt ctgcccggcc agtaacaatt gcatagcgga acacctcacc acagcgctgg
      781 cggaccttgc gcattttctc cagagctcca cgtttttcca ttttacgcag tgcttccagt
      841 aactccattg gtttgatttc ggcaataggg cgcttgccaa tgtagggaaa aatgtctttt
      901 tcaaaggtat caatgatttc atcacgatag cgtaatgacc accgatcagc ttttgactgg
      961 tgccattcgc gagcaatagc ctcgaacgta tttttcagac tcatttgctg cgcgattttt
     1021 tcttcctttt ttgcctcccc tggatcgcct ccagcagcga ggagtttcct ggcttcactg
     1081 cgtttctcgc gagcatcggc taacgttaca tcggggtata cgccaataga aagcatcttt
     1141 tctttaccag caaagcggta cttcatgcgc cagtatttag aaccattagt gttaatcagt
     1201 agatacatac cgccgccatc agcaagctta tagggtttgt ctttgggttt tgcggttttc
     1261 actttgatat cggttaatgc catgacatcc tccggctttt gatgaattca tcttcctgag
     1321 ggggatttct gccagtaact aacctgatac ccccaataca ccccttgtat aacaaagata
     1381 ttgttaaaag ccgttgttat gtatcatatt tttcctatta tgggggtgct tgtatttcaa
     1441 taggaaataa caccccctaa agtaccccca aaagaaactt gatatggggt gaagtaggtt
     1501 gacctcagga tacaaataaa tactgatata caagggatta gaagagattt attgacattg
     1561 ggagagttca gaagaggtaa actggcggaa gatcacagga gtcgaacctg cccgggaccg
     1621 ctggcggccc caactggatt tgaagtccag ccgcctcacc ggagacgacg atcttccgcg
     1681 cctcgattgc tacatggagg cggggcgcat tatagctact tccttgagtt tctacatccc
     1741 ccagatcagt tttttccgcc taatacatgc agcatattat cacgaaggca ttatagttca
     1801 tataaaacct atattttaca atgatatatg agcgtaattt acgctaccac gatcacataa
     1861 aacaagaatc ataaattaat aaccagatat cggaatattc gctctcacag ggatggttta
     1921 caaaatgcgt tatcggtctc agcaccccta tagcatcaag gaaaagcaga tgaagagtga
     1981 agtgttgtcc
//
LOCUS       DAPHBJ010000333         1975 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.336, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000333 DAPHBJ010000000
VERSION     DAPHBJ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.336"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(3..1673)
                     /locus_tag="RHZ76_004493"
     CDS             complement(3..1673)
                     /locus_tag="RHZ76_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV2814991.1"
                     /translation="MTKTSHSKIYSTESGITGGGFFKTHPLHFTKEQLEWIENIQLPK
                     RLFLISATEFDDRYLYLHDDWRYRFSGNNLAILFSTGKYFCDLSPVSLKLLKYLTIAY
                     INENSASVADKFAQFVATTFKQIEVITRESLIEKMQMLVAKTERNHSDSSQFYYTLYA
                     LRKLDRNGFFESKDDSDELEDLLLEVPRPRNNNWARYQNLDLVIPEEVCLMIENGIQR
                     WASKLCPKIDVEENKNIHLETVRKTIKIDTLRDCIIIGIVYYIGARPVQIGKLSGGDF
                     IVDTENKYGMRYSVLVPYAKKSKLTIDRVRVAIPEELGKLILLYKYLVGIGDTDPMFS
                     IIVSSMKMVEASLKKMLFRFSPQEIQEAVKNDDYQLPVYTASLFRHNVGHSMAMSGAS
                     APEIAYILGQSSYVVADRYIAATPELADIREAALGRNPVFKNMMALMLTGNLVHSSEW
                     EGRNVAGSIGGQLHYHLGGCNYEEDICPFAQGRGCYGCLYFKPFIDGNHKKVFLSLND
                     EIQNVRDVADDAGILNHPLLKELVRRKEHVNQVMARIEMAKAGGLFNR"
     gene            complement(1636..>1975)
                     /locus_tag="RHZ76_004494"
     CDS             complement(1636..>1975)
                     /locus_tag="RHZ76_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV2814992.1"
                     /translation="ANITLEFIYNSLLSESLILAQDYGIRARMNGVLEPAIEQLRALG
                     GWSLTSKVPLRYAKRFIEVVSNQSNYKRTKHSDMHLASPETNISQRKTSNLLEDNVYD
                     EDFTFKDLFN"
BASE COUNT          565 a          421 c          374 g          615 t
ORIGIN      
        1 agtcatcgat taaatagtcc tccggccttt gccatttcaa tgcgagccat aacctgattt
       61 acatgctctt tccgccgcac taattccttg agaagtggat gatttaaaat gccagcatca
      121 tcggcaacat cacgaacatt ttggatttca tcattcagcg aaagaaatac ttttttgtgg
      181 tttccatcta taaatggttt gaaatacagg caaccgtaac atccacgacc ctgtgcaaag
      241 gggcaaatgt cttcttcata attacatcca ccgagatggt aatgaagttg cccaccaatt
      301 gaccctgcaa cattgcgacc ttcccattcg ctgctgtgaa ctaaattccc agtcaacatg
      361 agtgccatca tgttcttaaa cacagggttg cgacccaaag ctgcctcacg aatatcagcc
      421 agctcagggg tggctgcaat gtatcgatct gcaacaacat aagaggattg tcccaatata
      481 taagctatct caggagcact ggcaccactc attgccattg agtgaccaac gttgtgacga
      541 aagaggcttg cggtatatac aggtagttga taatcatcgt tttttactgc ctcctgaatc
      601 tcttgtggtg aaaatctgaa aagcattttc tttaatgacg cttccaccat cttcattgaa
      661 gatactatga tggaaaacat cgggtcggta tcaccaattc caactaggta cttatacagt
      721 aagattaact ttccaagttc ttccggaatt gcaactcgaa ctcgatcgat agttagtttt
      781 gactttttgg catatggcac aagtactgaa taacgcatcc cgtacttatt ctcggtatca
      841 acgataaaat cgcctccgga aagtttaccg atctgtacag gtctggcccc aatgtaataa
      901 actatgccaa taataatgca gtcccgtaat gtatcaattt taatggtttt ccttacagtt
      961 tccagatgaa tgtttttatt ttcttcaaca tcaatttttg gacatagctt agatgcccaa
     1021 cgttgaatgc cattttctat cattaggcat acttcttctg gtataacgag gtctaggttt
     1081 tggtagcgag cccagttatt gttcctaggg cgtggcactt ccagcaatag atcttccagc
     1141 tcatcactgt catccttact ttcgaagaat ccattacgat caagttttcg taatgcatag
     1201 agggtgtaat agaactgact gctgtctgag tgatttcgct cagtcttcgc aactagcatt
     1261 tgcatttttt caataaggct ttcgcgagtg ataacctcaa tttgtttgaa agttgttgcg
     1321 acaaactgag caaatttatc agcaacactt gccgagttct cattaatgta cgcgatcgtt
     1381 aagtatttca agagcttgag tgaaactggt gataaatcac aaaagtattt accagtagag
     1441 aataatatag ccaaattatt acctgagaat ctgtaacgcc aatcatcatg tagatataaa
     1501 tatcggtcat caaattctgt tgcacttatt aaaaacaatc gtttagggag ctgaatattt
     1561 tcaatccatt caagctgttc ttttgtaaag tgaaggggat gggttttaaa aaaaccaccc
     1621 ccggtgatac cgctctcagt tgaatagatc tttgaatgtg aagtcttcgt cataaacatt
     1681 atcttctagc aagttggagg tttttctttg cgaaatgttt gtttctgggg aagctaaatg
     1741 catatctgaa tgtttcgtac gcttatagtt gctctggtta ctcaccactt caataaaccg
     1801 cttggcatat cttaatggaa ctttactggt cagtgaccag ccgcctaaag cacgaagttg
     1861 ttcaatagct ggttcaagta ctccattcat ccttgctcga ataccatagt cttgcgccag
     1921 tattagtgat tcactcagga gactattgta aataaactca agagtgatat tagcc
//
LOCUS       DAPHBJ010000334         1972 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.337, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000334 DAPHBJ010000000
VERSION     DAPHBJ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.337"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..750
                     /locus_tag="RHZ76_004495"
     CDS             <1..750
                     /locus_tag="RHZ76_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000045554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814993.1"
                     /translation="DLIVIMNEDGPTIIELNSLKSVSMLAQKLSAFSTYYHVEMQQIL
                     VNPIDFEKAYTLLKEAPAIPMFKTLADLDKFLNEEFEKYGLNTFLDVDNLDYSLAKSR
                     ELKNDQLVAWVSEIIEKREKLALRNRFNEVTKAHYETVDAMYAAVRPLMKELGFPDEL
                     MLHTFSELSVFDSKGWDYAIKSKIEFLTKREEQCLDYQMKADKRQATVDELLAQINNA
                     KTVKAPRSFGQLFGFSVIAMMTFMFIVNKFI"
     gene            809..1186
                     /locus_tag="RHZ76_004496"
     CDS             809..1186
                     /locus_tag="RHZ76_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814994.1"
                     /translation="MSYLEYVNEVKVSGIVISAMEKEMTSGNVGLLIKLKNRMKTEIN
                     GEITEREFSIQIKVSPEMYSGCFTGINQGDELMVSGYLVVDTITLEGREHPLDYMRVV
                     ATSKLAHIPKPPKGFGQSSFRQI"
     gene            1247..>1972
                     /locus_tag="RHZ76_004497"
     CDS             1247..>1972
                     /locus_tag="RHZ76_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814995.1"
                     /translation="MKQKIRPTAFCSKQMDFFSIFDFEEEEKEIITIVESPLDYINDA
                     IAAQQSIQSEDKHSGPELEPSVSEDVALPGNCPKLLKNQILHDISFAAETDYLFTLEE
                     YSAELGGLVIDWAPEDVYQLYVVAMEESLENVRHLVITKSLYSTDEFGNITVNPLLEA
                     ETRWYMSKSFELTCATHGIDAIEFRSELKKSLYEYTHSYGGENAELARYHQDKEVILH
                     DCKEEMGWDIFFEQDYLLQENKLA"
BASE COUNT          641 a          333 c          421 g          577 t
ORIGIN      
        1 gaccttattg ttattatgaa tgaagatggt ccaaccataa ttgaactgaa ttctttgaaa
       61 tcagtttcaa tgctggctca aaagctttct gctttttcta cttattatca tgtggaaatg
      121 cagcaaattc ttgtcaatcc aattgacttt gagaaagctt acacacttct caaagaagca
      181 cctgcaattc caatgtttaa aactttagcc gatctggata agttcttaaa tgaagagttt
      241 gaaaagtacg ggttgaacac atttcttgat gtggataacc ttgattattc actcgctaaa
      301 tccagagagt taaaaaatga tcagttggtt gcctgggttt ctgaaattat tgagaagcgc
      361 gagaaattag cattgcgtaa tcgatttaat gaagtgacca aagcacatta tgaaactgta
      421 gatgcaatgt atgcagccgt tcgcccatta atgaaagagt taggttttcc tgatgaactc
      481 atgttacaca cctttagtga attgagcgtt ttcgattcca aaggctggga ttatgctatc
      541 aagtcgaaaa ttgagtttct cacaaaacgc gaggagcaat gtttagatta tcaaatgaaa
      601 gcagataaac ggcaagcgac agttgatgaa ctcttggcac aaatcaataa cgctaaaacg
      661 gtaaaagcac caagatcttt tggtcagctg tttggctttt ctgttattgc catgatgaca
      721 tttatgttca ttgtaaacaa atttatctaa tcctgttaag gggaaaccct gacggggctt
      781 ctctttgctt aaattgagga gagaagtaat gtcatatctt gaatatgtta atgaagtaaa
      841 agtgtccggg atagttatca gtgcaatgga gaaagaaatg actagcggaa acgttgggtt
      901 attgataaag ctcaaaaaca gaatgaaaac tgaaattaat ggtgaaatta ctgagagaga
      961 attttcgatt caaatcaaag tatcaccaga gatgtattca ggctgtttca ctggtataaa
     1021 tcaaggtgat gagttaatgg tctccggtta tcttgttgtg gataccatca cattggaagg
     1081 gagagagcat cctcttgact acatgagagt agttgcaaca agtaagttag ctcacatacc
     1141 taaacctcct aagggttttg ggcaaagtag ctttagacag atctgacatt taaaccaccc
     1201 aaaaggggag aaatcccttc atgggagttc tcccttagga gatcccatga aacaaaaaat
     1261 aaggccgact gcattttgca gtaagcaaat ggattttttc tctatattcg attttgaaga
     1321 agaggaaaaa gaaataataa ctatagttga gtctccactc gactatataa atgatgcaat
     1381 agccgcacaa caaagtattc aatcagaaga taaacattca ggcccggaac tggagcctag
     1441 tgtgtcggaa gatgttgctt tgcctggtaa ttgtcccaaa cttctcaaaa atcaaattct
     1501 gcatgacatt agttttgctg cggaaactga ttatctcttc actctagaag agtacagtgc
     1561 ggagttaggt ggtctggtta ttgactgggc ccctgaagat gtatatcagt tatacgtcgt
     1621 cgcaatggaa gagtcgttgg aaaacgtaag gcatctcgtc attacaaaat cactctattc
     1681 cactgatgaa ttcggtaaca taaccgtgaa tccgcttctt gaagccgaaa cacggtggta
     1741 tatgtcgaaa tcatttgagc ttacatgtgc cactcatggt attgatgcta ttgagttcag
     1801 gtcagagctt aaaaaaagct tatatgaata tacgcacagt tacggaggtg aaaatgctga
     1861 attagctcgt tatcatcaag ataaagaggt tattctgcat gattgtaagg aagagatggg
     1921 atgggatatc tttttcgaac aggactattt gttgcaagaa aacaaactgg ca
//
LOCUS       DAPHBJ010000335         1965 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.338, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000335 DAPHBJ010000000
VERSION     DAPHBJ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.338"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(80..1492)
                     /gene="pykF"
                     /locus_tag="RHZ76_004498"
     CDS             complement(80..1492)
                     /gene="pykF"
                     /locus_tag="RHZ76_004498"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HDV2814996.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          500 a          454 c          474 g          537 t
ORIGIN      
        1 attgcttctg gttatcgatt aaataaaaaa agcgcccatc agggcgcttc gatatacaaa
       61 ttaattcaca aaagcaatat tacaggacgt gaacagatgc ggtgttagta gtgccgctcg
      121 gtaccagtgc accagaaacc ataactacaa cgtcaccttt gtgtgccaga ccgctctgca
      181 gagccagttc tttacccaga cggtagaaat catcagtaga agtgatctct ttaaccagct
      241 gcggcacaac gcctttgctc agtaccaact gatgagccgt tttttcgttg gtggtcagtg
      301 ccaggatggt ggcatccggg aagtatttac gtactgcgcg agcagattta cctccctgag
      361 tagcaaccac gatcagcgga gcatccagtt tttcagcagt ttcaacggca ccacggcata
      421 ccgcttcggt aatgcgcagt ttacggttgt cattgttgaa ctcgagacgg ctgttcatca
      481 cgcggtcggt acgttcgcag atggtcgcca tgatagaaac cgcttccagc gggtatttac
      541 cttttgcgga ttcaccagac agcatcactg catcagtacc gtcgaggatg gcgtttgcaa
      601 cgtcaccggc ttctgcgcga gtcgggcgtg ggtttttgat catggaatcc agcatctggg
      661 tcgcagtgat aacgacttta cgtgcacgga tacatttttc gatcatcatc ttctgggcga
      721 agataacttc ttctaccggg atttctacac ccaggtcgcc acgcgcaacc atgatgccgt
      781 cagaggcttc gaggatttcg tcgaagttgt tgaggccttc ctggttttcg attttggaga
      841 tgatgtggat gttttcgccg ccgtgcgctt tcaggtgctc acggatttcg ataacgtcag
      901 aacgcttacg aataaaggaa gcagcaacaa agtctacgcc ttgttcgcaa ccaaagatca
      961 ggtcctgttt gtctttttca gccagtgctg gcagagcaat ggaaacgcca ggcaggttca
     1021 cacctttgtt ttcgcccagg tcaccgttgt tcagcacttt acagataact ttgttacctt
     1081 caatggcggt aacttccata ccgatcagac catcgtcaac cagtacggtg ttgccaactg
     1141 acaggtcagt agtgaaacct tcatacgtta ccgcaaccat ttcgctgttg ccgataactg
     1201 atttatcagt ggtgaaagta aaggtctgac cagctttcag agaaacgtcg ttaccgcctt
     1261 ccagtttcat ggtgcggatt tccggacctt tggtatcaag caggatagcg gcggttttac
     1321 cagttttgct catcacgttg cgcagattct gaatgcgctg accgtgttct gcatagtcac
     1381 catgagagaa gttcagacgc ataacgttca tgccagcgtc cagcatttta gctaacatct
     1441 cttcagattc ggttttcggt ccgatggtgc aaacaatttt ggtctttttc atgacagtct
     1501 tagtctttaa gttgagaagg atgggagaaa cttgctttct gggcgcactg ccggaaagcg
     1561 aatccggtat tacgaaagtg gtggtgcctc gctctaagga taggtgacat cgaaaaagcg
     1621 tgcagaggaa tgtgtgcttg tgtctcagcc caacgtgagt gcctgttttt gagtcgttga
     1681 gttctacagt ccaaagtgct gaaaccattc aagagtcaat tggcgcgcat tatacgctga
     1741 aattaatcca aaaggaaaac aaaaacagcg tttcaaaaaa tatacaactt gttagacaat
     1801 aaattgttat caaagattta acgttccagg gaaaaggtta cgcaaaaact tcgctgtttg
     1861 cgcaacgtct ggtgccaaag ttgtcagtgt gaggataagt gaaagttcat ccggtgtata
     1921 aatatatcat cgctcaattg ctacagttaa aataatttat cgcgt
//
LOCUS       DAPHBJ010000336         1878 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.339, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000336 DAPHBJ010000000
VERSION     DAPHBJ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.339"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..715)
                     /locus_tag="RHZ76_004499"
     CDS             complement(<1..715)
                     /locus_tag="RHZ76_004499"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814997.1"
                     /translation="MNKFKLKTVDVWDTILRRDCHPDFIKRSTAYYFFLKFHSLHNVD
                     SVDQLFTKRVETERLIGKKNKDLGYDDEYLIQDVMLQWVENYISAEQYDTPAIAHELY
                     SWEIEQEKKHIYLDPGIEDFLAQYPADKTIFLSDFYTSSTDLTELLVSAGLDQSVISD
                     GVSSIDERLNKRSGRLFDFIQQKYQLAGVDWIHIGDNEWSDVQMPTSKGIKSIRYLPA
                     QQHQLREQKEFLWNKNEDLT"
     gene            complement(721..>1878)
                     /locus_tag="RHZ76_004500"
     CDS             complement(721..>1878)
                     /locus_tag="RHZ76_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HDV2814998.1"
                     /translation="IEGSGGHRTMLQHAAYLEKMGHTCTIFLEDKSGKTSGAEVIHKL
                     FGLKFKDVIYDWNNAQPCDAAVATIWYSAPFVAALPFDCKRLYFVQDFEAYFNPVGDA
                     YLMAENSYCYGLKPITIGRWLRYKLQNDFAVESYHFEFGANHNIYKRVPTIQKEKAVC
                     FIYQPDKPRRCSRIGIEALGIVKHCMPDVKIYLYGSSINDNLWFEHENLGLLDLPKCN
                     ELYNKCSVGLCISSSNPSRIPFEMMNAGLPVVEVWRENNLYDFSEDAMLLCKQTPESI
                     AAGIMKILNDADLAASMSSAGQKFMNGRTLETETEGFYKAFNCVMNEQAYTAEVISRS
                     YNKPPFEAPLARPDIANTLTFAHQSLLKRIALRLPLFVRTPLSALYFKLKN"
BASE COUNT          554 a          397 c          363 g          564 t
ORIGIN      
        1 ccgtgagatc ttcattttta ttccagagaa actctttttg ctcacgcagc tgatgctgtt
       61 gggctgggag ataccgaata ctttttatac cttttgatgt cggcatttgc acatcggacc
      121 attcattatc gccaatatgt atccagtcaa cgccggctaa ctgatatttt tgttgaataa
      181 aatcgaagag acgtccactt ctcttgttca gtcgctcgtc aattgaagaa acgccatctg
      241 atatgacaga ctgatccaat ccagcgctaa cgagtaactc tgttaaatca gtggatgaag
      301 tataaaaatc ggacagaaag attgtcttat cagcgggata ttgcgcaaga aagtcctcaa
      361 tacctggatc cagatagatg tgcttcttct cttgctcaat ttcccaggaa tatagctcat
      421 gtgctatcgc tggcgtatca tattgctctg cagagatata gttttctacc cattgaagca
      481 ttacatcctg aatgagatac tcatcatcat aaccaagatc tttgtttttc ttaccaatta
      541 agcgctctgt ttcaactcgc ttagtaaata gctggtcgac ggaatcaaca ttgtgtagcg
      601 aatgaaattt aaggaaaaaa taatatgcag ttgaccgctt tatgaagtct ggatggcaat
      661 cacgacgcag aattgtatcc catacatcaa cagttttgag tttaaattta ttcatctgga
      721 ttaattcttc aacttaaaat aaagcgcgct taaaggtgtg cgcacaaata acggtaaacg
      781 tagagcaatt ctctttaaca gactctgatg cgcgaatgtt aacgtgttgg caatatcagg
      841 tctggcaaga ggggcttcaa acgggggctt attatacgat cgtgaaatca cttctgctgt
      901 gtatgcctgc tcgttcatca cgcagttgaa cgctttgtaa aagccttctg tttccgtttc
      961 cagggtacgc ccattcataa acttttgacc agccgagctc atactcgcgg ctaaatcagc
     1021 atcgtttaga attttcataa taccggcggc aatgctttca ggtgtctgtt tacacaacag
     1081 catagcatct tcgctaaaat cataaagatt gttttctcgc cagacttcaa caacgggaag
     1141 gcctgcattc atcatttcaa aggggatgcg agagggattt gaagaactaa tacagagtcc
     1201 tacggagcat ttattatata gctcattaca tttcggcaaa tctaataaac ctagattttc
     1261 atgctcgaac cagagattat cattaattga tgaaccataa agataaattt taacatctgg
     1321 catgcaatgt ttaactatac ctaaagcctc aataccaata cgcgaacaac gtcgcggttt
     1381 atctggctga tagataaagc aaaccgcttt ttctttttga atggtgggaa cgcgcttata
     1441 gatattatga ttcgccccaa attcgaagtg atagctttca acagcaaaat cattttgcag
     1501 tttatatcgt aaccagcgcc caatcgtaat tggctttaag ccgtaacagt atgaattttc
     1561 agccatcagg taagcatcgc ccaccgggtt gaagtaagct tcaaaatcct gaacgaaata
     1621 taaacgttta caatcaaatg gtagagcggc cacgaatggt gcagaatacc atattgttgc
     1681 aactgcagca tcacatggtt gcgcattatt ccagtcataa atgacgtctt taaattttag
     1741 accaaataat ttatgaatta cctctgcacc gcttgttttg ccgcttttat cttcaagaaa
     1801 aattgtacat gtatgaccca ttttttccag ataagcggca tgttgcagca ttgtgcggtg
     1861 gccgccagac ccttcaat
//
LOCUS       DAPHBJ010000337         1858 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.340, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000337 DAPHBJ010000000
VERSION     DAPHBJ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.340"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..947)
                     /locus_tag="RHZ76_004501"
     CDS             complement(<1..947)
                     /locus_tag="RHZ76_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2814999.1"
                     /translation="MTNSTDNQNYVRAVLAGIGIDFDETEMFISVSHCQSDEVSFTCS
                     ISASELRESAGHYVDTLNDTQLAGLDADALKKRLVYFLEVFDLVSGQYLDISGKHFAT
                     SRFEYDDVCSEILSNSADSAQPGGYDREEYKRLMEVDGQVLIARFALEKFWDTHFIGL
                     INYVSDEITSGLYEVYRTFSDINMAGYTFSEYSYTRRITDELSLHISLKEDDFEEQLT
                     DCYMDETTLPSGKVVLRRNNESIIGIYEGYASKSYFPMVANVRVLDTDGEVVTELYQG
                     VNVSELAGGRIKIHDRQELISEVFANLREFIPASEDKIF"
     gene            complement(1124..>1858)
                     /locus_tag="RHZ76_004502"
     CDS             complement(1124..>1858)
                     /locus_tag="RHZ76_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815000.1"
                     /translation="PYSETEVLRTKIKYFLKVYEKSGFQNIRIRGYHNAHSTIDIVDI
                     ASLILAGSVPESEHDSIDPVLRKEIYQNRMSVEGKVLIARFALKQFFHSDFGDFILEF
                     EKSISKCLNTSLQIIKSVKNSFNRLGQYQYQRRVKDDLTLHLDLNTDEYPTCMPDLYI
                     GFKESEGTTGVYRDDEKIIRLYTGVSSGKDVPVMMTVRFTGCDGSVLSESSHGTYCSV
                     GPTGRVQVCDRVALVQEAVEELRDVV"
BASE COUNT          571 a          425 c          349 g          513 t
ORIGIN      
        1 tcaaagattt tatcctctga tgctgggatg aattcacgta agttagcgaa cacctcagaa
       61 attaattcct gacggtcatg gattttaatc cgaccaccag ctaattctga tacattcacc
      121 ccttggtaaa gttcagtaac aacttcacca tctgtatcaa ggactctaac attagcaacc
      181 ataggaaagt aagattttga agcatatcct tcataaatcc caataattga ttcattatta
      241 cggcgtaaaa ccaccttgcc agacggcagc gttgtttcat ccatataaca atcggttaat
      301 tgctcttcga aatcgtcctc tttaagtgaa atatgcaaac taagttcgtc ggtaatacga
      361 cgcgtatatg aatactcact aaatgtataa ccggccatgt ttatatcact gaacgtgcga
      421 taaacctcat aaagcccgga tgttatttca tctgatacat agttgataag cccaataaaa
      481 tgcgtatccc agaatttttc cagtgcaaat cgggcgatta aaacctgacc atcaacttcc
      541 atcagtcttt tgtactcttc acgatcatag ccacctggct gggcgctgtc cgcagagtta
      601 ctcaggatct cactacagac atcgtcgtat tcaaagcggc tggtcgcgaa atgtttaccg
      661 gaaatatcca gatattgacc ggaaaccagg tcgaaaacct caaggaagta aaccagtcgc
      721 ttcttgagtg catccgcatc caggccagcc agttgggtat cattaagagt atccacataa
      781 tgccccgcag attctcttaa ctcactggct gatatactgc atgtaaacga aacttcgtcg
      841 ctctggcaat gagagacact gataaacatc tctgtttcat caaaatcaat accaatgcct
      901 gccagtactg cacgaacgta gttctggtta tcagtagaat tagtcatctt cacttcctca
      961 ggattattaa aggaagtgcc ccacagggga gcgacttccc cagtgggtgg attaaaaata
     1021 tgtactactg cgccattctt tcctgcaagt tgatcagaat aggtagcgcg gtcatttggt
     1081 agaacaaagt tactttttgg aatgaagagg taaaaaacaa gggttaaaca acatcccgta
     1141 actcttcgac cgcttcttgg accaacgcaa ctctgtcaca aacctgcact cggccggtag
     1201 gtcccacgga acagtaagta ccgtgcgatg attcagacaa aacactcccg tcacagccag
     1261 tgaatcgaac ggtcatcatt acaggaacat cctttccgga actaacgcca gtataaagac
     1321 gaatgatttt ttcatcatcg cgatacactc ccgtcgtccc ttcagactcc ttgaaaccga
     1381 tgtaaaggtc tggcatacag gtcgggtatt catctgtatt caaatcaaga tgcaaagtca
     1441 ggtcatcttt tacacgacgc tgatattgat attgacctaa acggttaaat gagttcttta
     1501 cagatttaat tatctggaga gaagtattca aacacttgga gatacttttc tcgaactcta
     1561 agatgaaatc accaaaatca gaatggaaaa attgcttaag tgcaaaacgg gcaatgagaa
     1621 ctttaccctc aacactcatg cgattttgat agatttcctt tcttaaaaca ggatctattg
     1681 aatcatgttc agactccggt acagaccccg ccaatattaa tgatgcgata tcgacaatat
     1741 ctatagtgct gtgcgcgtta tgataaccgc gaattcttat gttctgaaaa ccagactttt
     1801 catatacttt aagaaagtat ttgattttag tccgaaggac ttcagtttca gaatacgg
//
LOCUS       DAPHBJ010000338         1854 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.341, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000338 DAPHBJ010000000
VERSION     DAPHBJ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.341"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            380..>1854
                     /locus_tag="RHZ76_004503"
     CDS             380..>1854
                     /locus_tag="RHZ76_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDV2815001.1"
                     /translation="MAGQYEKAITIKQAIDSINLRHYLLPAIQRKFVWSSSQICLLFD
                     SIMRDYPINSFMMWDIRSASIKNDYKFYEFLKEYCQRFNEENPCVATNAGFHDFKAVI
                     DGQQRLTSLYIGLCGTYAYKQPRMWWPSAQDDRILPPRKLYVDLTAPLKSDDELMMKY
                     NFRFLTEKQYTDSLADNKHHWFCLHEIFKYEQHDSPDDILFNVVVPELEKRDLISSEF
                     SRKTLLKLYTKIRTENLIHYFNESSQDIDHVLDVFIRTNSGGTKLEFSDLLMSIAVAH
                     WQGDFRKELDELTKNIYQNNEMGFYIERDWFLKTSLMLIDSDVRFKVKNFTSEEVGKI
                     QQQWSEIKSCIKETFILIRRFGINPQSLISKNAVIPVVYWLYKKQTSGHPLYTTINLL
                     NKNHNERSVISQWFYMVLLKGIFGSQADALLTSIREVMKNSLSDIHFPLEKIIDRYKG
                     SNKDLRFDDEYIESLLNIRYGEGRCRALLHLLFPEMNPTEV"
BASE COUNT          607 a          375 c          375 g          497 t
ORIGIN      
        1 atcgataaag aatacgcact tgtaaatgca acagcaagac ttaacactga cctgagggat
       61 tatgaatatg aaataaacaa cgccgccatc ataacatttg gcaatgacct cattgaggta
      121 atcgtatatc agttcagctt tgtcatcagc atcagggcag agggtgaaaa aataaagcac
      181 ggcctacttg ttaacttcgg taaaaatata gcaagacagg tttcatcact ttgtgcatcc
      241 gcaatgcgag tctacccgaa tgaaaaacac aaaccgagcc gtcagctttt ccattgcata
      301 aattagtttg tgcaaagact gtactaccga cacgcaataa attaaaatag gccagctaat
      361 ataacatatg aggtttatga tggctggcca gtatgaaaaa gcaataacga taaaacaagc
      421 gatagattca attaatttac gccactatct tctgcctgca attcagagga agttcgtctg
      481 gagcagcagt cagatatgtt tgctgtttga ttccatcatg cgagactacc cgataaactc
      541 tttcatgatg tgggatatcc gtagtgccag tattaaaaat gattacaaat tctatgagtt
      601 cctgaaagaa tactgtcagc gatttaatga agaaaacccc tgcgtagcaa caaatgctgg
      661 gtttcatgat ttcaaggccg tgattgatgg tcagcagcgt ctcacatcat tatatattgg
      721 attgtgtgga acgtatgcgt ataaacagcc ccggatgtgg tggccttcgg cacaggatga
      781 tcgcatcctg ccacccagaa agctttacgt cgatttaaca gcgccactta agtcagacga
      841 tgagctcatg atgaagtata acttcaggtt cctgaccgag aagcaatata ctgattcact
      901 tgccgataac aaacatcact ggttctgcct gcacgaaata ttcaaatacg agcaacatga
      961 ttcgcctgat gatattttat ttaacgttgt cgtaccagaa cttgaaaaaa gagatctcat
     1021 ttccagtgaa ttttccagaa aaactctgct taaactttat accaagataa gaactgagaa
     1081 tcttatccac tacttcaatg agagcagtca ggacattgat catgtgctgg atgttttcat
     1141 ccgcacgaac agcgggggga caaaacttga gttctccgac ttactgatgt caatagcggt
     1201 agcgcactgg cagggtgatt tcagaaaaga actggatgaa ctgacaaaaa acatttatca
     1261 gaataatgaa atgggttttt atattgaaag agactggttc ttaaaaacca gcctgatgct
     1321 tattgactct gacgtccggt tcaaagtaaa aaacttcact tcagaggagg tcggtaagat
     1381 acaacaacaa tggtctgaaa taaaatcctg tatcaaggag acctttattc ttatcaggcg
     1441 attcggcatc aatccacagt ctttgatatc taaaaatgca gtcattcctg tggtctactg
     1501 gctttacaaa aagcaaacca gtggacatcc attatataca acgattaatc tcctgaataa
     1561 aaaccacaat gaacgctcag taattagcca gtggttttac atggtacttc tgaaagggat
     1621 ctttggaagc caggcagatg cgctactcac gagtatcaga gaggtaatga aaaatagtct
     1681 ttcagatatt cactttcctc ttgaaaagat tattgacaga tataaaggct cgaataaaga
     1741 cctcagattt gacgacgaat acatcgaaag ccttctcaat atcagatatg gcgaaggtcg
     1801 ttgccgcgca ctattgcatc ttctgttccc tgaaatgaat ccgaccgagg tgtt
//
LOCUS       DAPHBJ010000339         1818 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.342, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000339 DAPHBJ010000000
VERSION     DAPHBJ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.342"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            158..661
                     /locus_tag="RHZ76_004504"
     CDS             158..661
                     /locus_tag="RHZ76_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HDV2815002.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(740..991)
                     /locus_tag="RHZ76_004505"
     CDS             complement(740..991)
                     /locus_tag="RHZ76_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HDV2815003.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1106..1192)
                     /gene="azuC"
                     /locus_tag="RHZ76_004506"
     CDS             complement(1106..1192)
                     /gene="azuC"
                     /locus_tag="RHZ76_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HDV2815004.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1455..1778
                     /locus_tag="RHZ76_004507"
     CDS             1455..1778
                     /locus_tag="RHZ76_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HDV2815005.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          472 a          431 c          404 g          511 t
ORIGIN      
        1 tatacccgtt cagacgttat tcttatttca gatcatcgtc agaattgact ccacgatcac
       61 atttcggacc ggcagaaagg aattattctg caaacagtaa ttatggtgtt ttgatttatc
      121 ttgcacctct ccacttctgg atataaggat attaggtatg gcaaccgctg gaatgcttct
      181 caaactcaac tctcaaatga accgcgagtt ttacgcatcc aatctctacc ttcacctgag
      241 taactggtgt tctgaacaga gtctgaacgg caccgccact ttccttcgcg cccaggcaca
      301 gagtaatgtg acccaaatga tgcgcatgtt taactttatg aagagtgtcg gcgctacccc
      361 catcgttaaa gccattgatg ttcccggtga aaaactgaac tctctggaag aactgttcca
      421 aaaaacgatg gaagaatacg agcaacgttc tagtacgttg gcacagttag ccgatgaagc
      481 gaaagaactg aatgatgatt caaccgtcaa tttcctgcgc gatctggaaa aagaacagca
      541 gcatgatggt ctgttgctgc aaaccattct tgatgaagtg cgcagtgcga aacttgcggg
      601 tatgtgccct gtgcagaccg accaacatgt tctgaatgtc gtgtcacacc agctgcattg
      661 atcatcatcg gcgctaatgc attgcgccga tgaaggtttt gagaaaccgc tgcctcatct
      721 gtttgaagca gcggtttttt taatgggatt caccctgtgg ggtaaacttg agttcaataa
      781 gcgcgatggc tttttggatt gcccgcatgg tgaccgggtc tgcggcggcg ggatggttag
      841 taaagtcgat attcttcagc tgactggaca ttttttcacg aacttcaacg ggcgcgatta
      901 catcgagaac atccagaatt tgtttgataa ccaactggca agcaaccaca tcagaaacca
      961 gttcctgatc ggcattcagc ggctgggaca tcgtaaactc ctgatagcat tttgaaagcc
     1021 gttatagtag cggcttcaca tcttcagcga tagtcacatc caccgtcatc aggacacaaa
     1081 aaaacctgcc ggagcaggtt ttttattatc ggaacatatt gcctggcggt acgtctttga
     1141 acgtcttgca atagttattg aacatacttt tcaggatttt gcgcagtttc atcgcggcac
     1201 tccgaccatt tgttatacag gtgttattgt ctttgcgctt ataatatgac aaccatcaca
     1261 aaaatcaatc tttatgtgat acaaatcaca taaatacccc tttaatgtta taaaaatgat
     1321 aatcaaaaaa cagcccccct atttctgaca cctacagatg gcaagaaata gcgcctgcca
     1381 ggcgtctttt ccggccattg tcgcagcgct gtaacgcgta aaatagtgct ttctcttact
     1441 cttctggctg gaccatgaga cttctgattc tgactctttc attaataacg ctggcggggt
     1501 gtacggttac tcgccaggcc catgtgagcg aagttgacgc ggcaacaggt attgtacggt
     1561 tggtttatga ccaggctttt ttgcagcatg cccataccga tcgttatgtc agtcgcggga
     1621 tcgccgatcg cgcatgccag caggaaggct atacccacgc ggtccccttt ggtcagccag
     1681 taggcaactg tagccttttt gcgggttcct tatgtttgaa tacggaattc actttgtcat
     1741 atcagtgcca tcattccgcc ttccctgtct ttctctaaat attttaatca gcgaggggat
     1801 cttcgctgat taaagaaa
//
LOCUS       DAPHBJ010000340         1774 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.343, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000340 DAPHBJ010000000
VERSION     DAPHBJ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.343"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1362
                     /locus_tag="RHZ76_004508"
     CDS             <1..1362
                     /locus_tag="RHZ76_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDV2815006.1"
                     /translation="EKTDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYAEPLVES
                     RYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRSRIQTIY
                     QPGSFTPLIRVETATGELARTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEI
                     LADRVSEESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLPLALVSTEGAT
                     EWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDP
                     IGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKPPTPDPSKLPDIAKQ
                     LRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCTPQDDSQYPGMDTKT
                     ASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDLGEILAKMKQRTSRG
                     IRK"
     gene            1359..1774
                     /locus_tag="RHZ76_004509"
                     /pseudo
     CDS             1359..1774
                     /locus_tag="RHZ76_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          506 a          417 c          477 g          374 t
ORIGIN      
        1 gagaaaaccg acctcatccc ggaaggggtt atccgcacgg atgatgagcg cacccaccgg
       61 taccattacg acagtcagca ccggctggtg cactacacgc ggacacaata tgcagagccg
      121 ctggtcgaaa gccgctatct ttacgacccg ctgggccgca gggtggcaaa acgggtatgg
      181 cgacgtgaac gggacctgac gggctggatg tcgctgtcac ggaaaccgca agtgacctgg
      241 tacggctggg acggcgaccg gctgaccacg atacagaacg acaggagccg catccagacg
      301 atttatcagc cggggagctt cacaccactc atcagagttg aaactgccac cggtgaactg
      361 gccagaacgc agcgccgcag cctggcggat gcgcttcagc agtccggcgg cgaagacggt
      421 ggcagtgtgg tgttcccgcc ggtgctggtg cagatgctcg accggctgga aagtgaaatc
      481 ctggctgacc gggtgagtga ggaaagccgc cgctggctgg catcgtgcgg cctgaccgtg
      541 gagcagatgc aaaaccagat ggacccggtg tacacgccgg cgcgaaaaat ccacctgtac
      601 cactgcgacc atcgcggcct gccgctggcg cttgtcagca cggaaggggc aacagaatgg
      661 tgcgcagaat acgatgaatg gggcaacctg ctgaatgaag agaacccaca tcagctgcag
      721 cagcttatcc gcctgccggg gcagcagtat gatgaggagt ccggcctgta ttacaaccgc
      781 caccgctatt atgacccgct gcaggggagg tatatcactc aggatccgat tgggctgaag
      841 gggggatgga atttttatca gtatccgctg aatccggttc agtatataga ttcaatggga
      901 ctggcatcaa aatatggaca cttaaataat ggcggatatg gagcgagacc caacaaaccg
      961 cctacgcccg atccaagtaa attgccggac atagcgaaac aattaagact gccatatcct
     1021 attgaccagg ccagtagtgc gcctaatgtt ttcaaaacat tcttcagagc attaagccct
     1081 tacgactaca cactgtattg caggaagtgg gtaaaaccaa atctgacttg tacgccacag
     1141 gatgattccc agtatccagg gatggataca aagacagcaa gtgattacct gccacagaca
     1201 aattggccaa caactcaatt accaccagga tatacttgtg cagaacccta tttattccca
     1261 gacattaata aacccgatgg gccagcaaca gcagggatag atgatttggg tgaaatttta
     1321 gctaagatga aacagagaac atcgagagga ataagaaaat gaaaagagtt ttgttctttt
     1381 tgctgatgat atttgttagt tttggtgtta tagctgattg cgaaatacaa gctaaagatc
     1441 atgattgttt tactattttc gctaagggta ctattttttc cgcgtttcct gtcttaaata
     1501 ataaagccat gtggagatgg tatcagaatg aggacatcgg tgagtattat tgacaaacag
     1561 aactgggcac atgtaaaaat aataaattca caccaagcgg ggcgagactc ttaattcgcg
     1621 ttgggtcact acgccttaat gaaaatcatg ccatcaaagg aacgttgcag gagttgataa
     1681 atacagcaga aaaaacggcg tttctcggcg atcggtttag aagttatata agagcgggta
     1741 tctatcaaaa aaagagcagt gatccagctc agtt
//
LOCUS       DAPHBJ010000341         1766 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.344, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000341 DAPHBJ010000000
VERSION     DAPHBJ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.344"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            25..846
                     /locus_tag="RHZ76_004510"
     CDS             25..846
                     /locus_tag="RHZ76_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815007.1"
                     /translation="MQNDLGLGFIRKSARTSAKIISRKVTFPLDDLDNAKLTLTGLQC
                     HPSCRFPFYQLSMLIHNQLAEHGVISADENAPFTVSISIHEWLSRARVAFIDYHGLRN
                     SDKTVMITRAHLEELLTYLSTTLAVYVPYSKKRLNFSFLTSFTLVKTSQSYTLTFPGV
                     LRPFLMLLGGLIQEFMTEKLLKRRNAQFVIFDYLRKSRRHSHKITDIVNDLQLHTVNV
                     RIMNVLTQLADQGFISFICEGKRGERSIEELQFIPYASRDHSEVLSFEEWISPID"
     gene            complement(861..1685)
                     /locus_tag="RHZ76_004511"
     CDS             complement(861..1685)
                     /locus_tag="RHZ76_004511"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HDV2815008.1"
                     /translation="MRKHTPIIKWAGGKTKLMPFLSKHFPHDKSRRWVEPFIGGGAVF
                     LNMFATEALLADSNPDLINLYRNIQRNKPAFIREVQLLAERHFEEEDYYVLRNTFNST
                     SFDDAPLQRAAIFYAMNRLGYNGLCRYNLKRKFSVPWGKRYQFSLDIQKVDYLSFRLS
                     SVELKTADFGQTLEFAGGGDQIYCDPPYDKISKTSFVSYDGIPFDKSAHVKLADMLVD
                     ANRKGASVAISNSMTPFTLELYEERGFDIHTHNAYRSVGSQSKSRKKEIEILAVLR"
BASE COUNT          450 a          419 c          399 g          498 t
ORIGIN      
        1 gattttgtta aaacggagtg atatatgcaa aatgatcttg gacttgggtt catccgcaag
       61 tcagcaagaa cgtcagcaaa aattatttcc cgaaaagtta cttttccgct cgatgacttg
      121 gataatgcaa agctaacgct aactggccta cagtgtcatc catcctgccg ttttccattt
      181 taccagctgt ctatgcttat ccataaccaa ctcgctgagc acggcgtcat ttctgccgat
      241 gaaaacgcac cgttcactgt atccataagc attcacgagt ggctgagtcg tgccagagtg
      301 gcatttattg attatcacgg tcttcgtaat agcgataaga cggtcatgat cacacgcgct
      361 catctggaag aattgcttac ttacttgtct acgacattgg ctgtttatgt cccgtattca
      421 aagaagcgac taaacttcag cttcctgacg tccttcactc tggtcaaaac atcgcagtct
      481 tatacgttaa cgttccctgg ggtacttcgt ccgttcctga tgttgctggg gggattaatt
      541 caggaattta tgacggaaaa attattgaag cgacggaatg cccagtttgt catctttgat
      601 tatttgcgaa agtcccggcg ccattcacac aaaattaccg atatcgtgaa tgatcttcag
      661 ttgcatacag tgaacgtacg aatcatgaat gtccttaccc aacttgcaga tcaggggttc
      721 atatcgttta tatgcgaagg taagagaggg gagcgatcaa ttgaagagct tcagtttatt
      781 ccctatgcca gccgtgacca ttccgaagtg ctgagcttcg aggaatggat aagcccgatc
      841 gactaactac tgatcgccgc tcaacgaagg acggccagaa tttctatttc cttcttacgc
      901 gactttgatt gactgccgac agaccgataa gcgttgtgag tatgaatatc aaaaccgcgt
      961 tcttcataga gctccagcgt aaagggagtc atgctgttgg atatggccac tgaggcccct
     1021 ttacgattag catccaccag catatccgcc agtttaacat gtgcgctctt atcgaacggt
     1081 ataccatcat agcttacaaa gctggtttta ctgattttgt catatggcgg atcgcaatat
     1141 atttggtcac caccacctgc aaactccagc gtctgaccaa aatctgcggt tttcaattca
     1201 acgctcgaaa gcctgaatga gaggtaatca accttctgga tatccagact gaactggtat
     1261 cgctttcccc atggaactga gaactttcgc ttcaggttgt aacgacacag accgttatat
     1321 ccgagtcgat tcatcgcgta gaaaatggct gctcgttgta gcggggcatc atcaaaactg
     1381 gtgctgttaa aggtattacg cagcacgtag taatcctctt cttcaaaatg cctttccgcc
     1441 agcagctgca cttctctgat gaatgccggt ttatttcgct gtatgttgcg gtacagattg
     1501 attaggtccg ggttgctgtc tgctaggaga gcctcggtgg caaacatgtt cagaaacacg
     1561 gcgccaccgc cgataaacgg ttcaacccag cgtcggctct tatcatgtgg gaagtgctta
     1621 ctgaggaacg gcatcagttt ggttttaccc cccgcccatt tgatgattgg tgtatgtttt
     1681 cgcaacgagt cttctccgtt cagctttatc ggtatgccac acttatggta ccgaaaggcg
     1741 gattgctacc aataaggtat gagcag
//
LOCUS       DAPHBJ010000342         1765 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.345, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000342 DAPHBJ010000000
VERSION     DAPHBJ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.345"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..223)
                     /locus_tag="RHZ76_004512"
     CDS             complement(<1..223)
                     /locus_tag="RHZ76_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HDV2815009.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESV"
     gene            complement(297..1241)
                     /gene="yfdV"
                     /locus_tag="RHZ76_004513"
     CDS             complement(297..1241)
                     /gene="yfdV"
                     /locus_tag="RHZ76_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HDV2815010.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(1311..>1765)
                     /locus_tag="RHZ76_004514"
     CDS             complement(1311..>1765)
                     /locus_tag="RHZ76_004514"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="catalyzes the formation of formyl-CoA from
                     oxalyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HDV2815011.1"
                     /translation="LDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAFGFSGMEIE
                     TICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLMHHARYDKLMDAFRGVGYNV
                     TTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITQLNPKQVAGN"
BASE COUNT          550 a          428 c          365 g          422 t
ORIGIN      
        1 ccacactctc tttgccatga ttaataaaac tgtaatagag tgactgtcca tccacatagg
       61 gaccaaatgt gcgggtatca tcaccatgac ccggcggctc aactttaatt acccttgcgc
      121 ccatattaca aagaagttga gttccgaaag gtccattaag gacatgtgtc atatcgataa
      181 ctaataagcc ttcaaacggc cctttgcttt cattatttgt catttgctta tgccttgaaa
      241 aaagccgaag gtgaaaagac acttcggcat ttatgtaaaa tcaataacat gattaattat
      301 gaaaccagtc gactgacata aatccacaag ggagccgtga cgacaaagcc cagtacgctc
      361 acagccaatg acgctgtacc agtacgggtg tagacattaa accggctggc aataatgatt
      421 ccggagaatg ccggcggtaa tgcgcctgcc agtaccatca tctgcagatg ttcgctgttc
      481 aaatgacatg ccataccaac gagaagcagt gccagtggca tcagaatcag cttcaggaag
      541 gtgttataag caatttcggc actgaactcg aatttatgtg cagccagagt caaccccgca
      601 gcgaataccg ctacccctga gttagccttc gcaatcagat taaaggttgg gtcccatgct
      661 gccggaattt ttaccccaac caacaccagg atcgttgcca gaacaggtgc ccatactact
      721 ggctcctttg ccgcagaaat taatgcgctc agattactat tcttcttacc atccgctcct
      781 gaggaaggat tcagcaaata cagaccaata ggaatagtaa ttgcgttaac aataatagaa
      841 ataattgcta ccactaaacc tgttgatacg gaatcaccat aaataggatc gagaactgca
      901 aaccccagga atccaatggt aggtgaaccc gcaattaatg cacatacagc tgcttctgca
      961 tgggtacgtt taaaaaattt gtagcaacca aaccaggaga aaaagaaaca tccgacaata
     1021 accacaagtg atacaagggt cagacgagtg tccgcaaaaa tcatttccct gtttgcccgg
     1081 gtaatagaaa caaatagagc cgcaggaagc gcgtagttta ataccagttt attaaatgcg
     1141 cgggcttgat cttctgaaaa agtttctcgt ctgccgctaa aataacccaa cagcatgatt
     1201 acgataatag gcaataaatc gccaataaaa aatgttagca taatagtatt tccgataagg
     1261 gtcccctcac catcgcataa tgagttggca agaggagacg atgtcagtat ttaattacca
     1321 gcgacttgtt ttgggttaag ttgggtaata tggccacttt cagttcctgc tgcagggtcg
     1381 atgaccacat taataatggt cggtttgcgc gactgaatac cggtggttaa agcatgacga
     1441 agttcatctg tcgtggtgac gttatagcca acgccacgaa acgcatccat taatttgtca
     1501 taccttgcat ggtgcatcag atccgttggt gatggtgcac cagcgccact gagatcaaca
     1561 ccgtctcctc tgtagatgcc gccattatta aaaataacga tcgtcaccgg caggttatat
     1621 cgacaaatcg tttcaatttc catcccactg aaaccaaaag cactatcacc ttcaatggcg
     1681 acaaccggag aaccagaagt cacgctagca ccgatagcat agcccatacc gatgcccatg
     1741 acaccccagg taccacaatc cagac
//
LOCUS       DAPHBJ010000343         1711 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.346, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000343 DAPHBJ010000000
VERSION     DAPHBJ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.346"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..715
                     /locus_tag="RHZ76_004515"
     CDS             <1..715
                     /locus_tag="RHZ76_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDV2815012.1"
                     /translation="ETGAEIAGLRLIDRFVGTIAPIFRSSRLEVDYHYNPPGIRGEPR
                     YLEGVARFTKLHGSLDWYATEGAIRRFGLPFGASSVEPFLQVETTGTANYQQLMIYPN
                     SVKDRETSEYPYVELFRDLASATCRPNSTLVTYGYSFGDEHINRVIIDMLTIPSTHIV
                     IIAYGDPLGRIMRFINDSGRKAQISLLLGDHFGDIKKLVDFYLPKAAIDRSSIRMAEL
                     LKSRGLYRATTSNNSGDSE"
     gene            712..>1711
                     /locus_tag="RHZ76_004516"
     CDS             712..>1711
                     /locus_tag="RHZ76_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDV2815013.1"
                     /translation="MNSFPIEQGEQLRVGTVDFVSPNEIRALLDIDSPDAVALNAGTP
                     RNFPRVNSYVLVSCDNGYLVGQIEWLAVEHSPYPKQRDIQDFGLVNLPFPRKKISLNP
                     VGTLKRFSKDGTDYFKFQRGSESFPSIGSAILLPTDLQLRSIVESGDNRRIVIGQSPL
                     ANNANVAVDPDRLFGRHIAVLGNTGSGKSCSVAGLIQWSLEAAMESEVKPNARFIILD
                     PNGEYVRALGPTTKFKGRIFKVEAGENEHQLQVPSWFWNSSEWASFTQASPKAQLPLL
                     KRSLRAMRNEDFELETTSNIEIKRFLGNVLITLKHDKSSGKPFGAWGPAKGFNEIMG"
BASE COUNT          530 a          302 c          363 g          516 t
ORIGIN      
        1 tgaaacgggc gctgaaatag caggtcttcg tttaattgac agatttgtcg gtactatagc
       61 accaattttt agatcttctc ggttagaagt ggattatcat tacaacccac cagggattcg
      121 gggtgagcca agatatttgg aaggtgtggc gcgatttact aaattacatg gttcattgga
      181 ctggtatgct actgaagggg ctataaggcg ttttggattg ccatttggag ccagttcggt
      241 tgaacccttt ttacaggtgg aaactaccgg aactgcaaat tatcaacaat taatgattta
      301 tccaaattca gttaaagacc gtgaaacatc tgaatatcct tatgttgagc tatttaggga
      361 tttagcatct gcaacatgtc gtcctaatag caccttggta acatatggtt atagttttgg
      421 cgatgagcat ataaatcggg ttattattga catgttgaca ataccctcaa cgcatatagt
      481 aataattgca tacggtgatc cacttggtag aatcatgcgt tttataaacg atagtggaag
      541 aaaagctcaa atatctttat tgcttggtga tcactttgga gatataaaga aattagtaga
      601 tttttatctt cctaaagccg cgatagacag atcatcaatt cgtatggcgg agctattgaa
      661 gtcaagaggt ttgtatcgtg caactacctc aaataatagt ggggattccg aatgaactca
      721 tttcccattg aacaaggtga gcaactcagg gtaggtactg tagattttgt ttcacccaat
      781 gaaattagag cactattaga tatcgattca cctgatgctg tcgctttgaa tgcaggcaca
      841 cctagaaatt ttcctagagt aaacagctat gtattagtct catgtgataa tggttactta
      901 gttgggcaaa tagagtggct tgcagttgag cattcacctt atcctaaaca aagagatatt
      961 caagattttg gtctagtaaa cctaccattt cctcgaaaaa aaataagttt aaatccagtc
     1021 ggaactctta aacgcttttc caaagatggt actgattatt ttaaatttca acgcggctct
     1081 gaatcatttc cttctattgg ttcagctatt ttactaccaa cagatttaca gcttagatca
     1141 attgtcgagt ctggtgataa tagacgaata gttattgggc aaagtccact tgctaacaat
     1201 gctaatgtag ccgtagaccc tgatagatta tttggtcgtc atatagcagt acttggtaat
     1261 actggtagtg gtaagtcttg ttctgtcgca ggattaatcc aatggtcact cgaagctgca
     1321 atggagtcag aagtaaaacc aaacgccaga tttattatat tagaccccaa tggcgagtat
     1381 gtacgtgctt tgggaccaac tacaaaattt aaaggcagaa tttttaaagt cgaagctggg
     1441 gaaaatgagc atcaactcca agtgccatct tggttttgga atagctcgga atgggcatca
     1501 tttacgcaag cgagtcctaa agcccaactt cctttactaa agcgttctct aagggcaatg
     1561 agaaacgaag attttgaact tgaaacaacc tcaaatatag aaataaaacg ttttctaggg
     1621 aatgttctca taaccttaaa acatgataaa agttcaggga aaccctttgg agcttggggg
     1681 cctgcaaaag gctttaacga gataatgggt a
//
LOCUS       DAPHBJ010000344         1700 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.347, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000344 DAPHBJ010000000
VERSION     DAPHBJ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.347"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(86..1369)
                     /gene="gltA"
                     /locus_tag="RHZ76_004517"
     CDS             complement(86..1369)
                     /gene="gltA"
                     /locus_tag="RHZ76_004517"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HDV2815014.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1575..1682)
                     /locus_tag="RHZ76_004518"
     CDS             complement(1575..1682)
                     /locus_tag="RHZ76_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815015.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
BASE COUNT          445 a          405 c          412 g          438 t
ORIGIN      
        1 tacaattttt tatgttcagc catataaaaa agaacccgct ctattaaagg cgggtccgga
       61 aagtaaacgg cttagcaatc aatcattaac gcttgatatc gcttttaaag tcgcgttttt
      121 catatcctgt atacagctga cgcggacggg caatcttcat accgtcactg tgcatctcgc
      181 tccagtgggc gatccagcca acggtacgtg ccattgcgaa aatgacggtg aacatggaag
      241 acggaatacc catcgctttc aggatgatac cagagtagaa atcgacgttc gggtacagtt
      301 tcttctcgat aaagtacggg tcgttcagcg cgatgttttc cagctccata gccacttcca
      361 gcaggtcatc cttcgtgccc agctctttca gcacttcatg gcaggtttca cgcattacgg
      421 tggcgcgcgg gtcgtaattt ttgtacacgc ggtgaccgaa gcccatcagg cggaaagaat
      481 catttttatc tttcgcacga cgaacaaatt ccggaatgtg tttaacggag ctgatttctt
      541 ccagcatttt cagcgctgct tcgttagcac caccgtgcgc aggtccccac agtgaagcaa
      601 tacctgctgc gatacaggca aacgggttcg cacccgaaga gccagcggta cgcacggtgg
      661 aggtggaggc gttctgttca tggtcagcgt gcaggatcaa aatacggtcc atagcacgtt
      721 ccagaatcgg attaacttca tacggttcgc acggcgtaga gaacatcata ttcaggaagt
      781 taccggcgta ggagagatcg ttgcgcgggt aaacaaatgg ctgaccaatg gaatacttgt
      841 aacacatcgc tgccatggtc ggcattttcg acagcaggcg gaacgcggca atttcacgat
      901 gacgaggatt gttaacatcc agcgagtcgt gatagaacgc cgccagcgcg ccggtaatac
      961 cacacatgac tgccattgga tgtgagtcgc gacggaaagc gtggaacaga cgggtaatct
     1021 gctcgtggat catggtatga cgggtcaccg tagttttaaa ttcgtcatac tgttcctgag
     1081 tcggtttttc accattcagc aggatgtaac aaacttccag gtagttagaa tcggtcgcca
     1141 gctgatcgat cgggaaaccg cggtgcagca aaataccttc atcaccatca ataaaagtaa
     1201 ttttagattc gcaggatgcg gttgaagtga agcctgggtc aaaggtgaac acaccttttg
     1261 aaccgagagt acggatatca ataacatctt gacccagcgt gcctttcagc acatccagtt
     1321 caacagctgt gtccccgttg agggtgagtt ttgcttttgt atcagccatt taaggtctcc
     1381 ttagcgcctt attacgtaag actgccggaa cttaaatttg ccttcgcaca tcaacctggc
     1441 tttacccgtt tgttatttgg ctcgccgctc tgtgaaagag gggaaaacct gggtacagag
     1501 ctctgggcgc ttgcaggtaa aggatccatt gatgacgaat aaatggcgaa tcaagtactt
     1561 agcaatccga attattaaac ttgtctacca ctaataactg tcccgaatga attggtcaat
     1621 acttccacac tgttacataa gttaatctta ggtgaaatac cgacttcata acttttacgc
     1681 attatatgct tttcctggta
//
LOCUS       DAPHBJ010000345         1695 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.348, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000345 DAPHBJ010000000
VERSION     DAPHBJ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.348"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            148..909
                     /gene="envY"
                     /locus_tag="RHZ76_004519"
     CDS             148..909
                     /gene="envY"
                     /locus_tag="RHZ76_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HDV2815016.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(955..1031)
                     /locus_tag="RHZ76_004520"
     tRNA            complement(955..1031)
                     /locus_tag="RHZ76_004520"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(995..997),aa:Arg,seq:tct)
     gene            1274..>1695
                     /gene="fimZ"
                     /locus_tag="RHZ76_004521"
     CDS             1274..>1695
                     /gene="fimZ"
                     /locus_tag="RHZ76_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HDV2815017.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKC"
BASE COUNT          495 a          388 c          367 g          445 t
ORIGIN      
        1 cctctttcgc tctcgtcaga attccatcaa gattaattgc taaaaagcgt gctaaagaaa
       61 gaagatcgtt atcggcgaac gggccgatca ctcatgcagg aagcatagat cctttgagcg
      121 gagtttctga tatctgagga gtgcgaaatg caattgagca gcagtgaacc ttgcgtggtg
      181 atcctgaccg aaaaagaggt agaggtaagc gtcaataacc atgctacgtt tacccttccg
      241 aaaaactacc tggccgcctt cgcgtgcaac aataacgtca ttgaactctc aacgttaaat
      301 cacgtattaa tcacccacat caaccgtaac atcatcaacg attatctgtt gtttttaaat
      361 aagaacttaa cctgtgtaaa gccctggtcg cggctggcaa ccccggttat cgcttgtcat
      421 agccgtacac cggaagtgtt ccggctagcc gccaaccaca gcaagcagca acccagcaaa
      481 ccctgcgaag cggagttgac gcgcgcatta ctttttaccg tattgtctaa ctttcttgag
      541 caatcgcggt ttattgccct actgatgtat atcttacgca gcagcgtccg cgacagcgtc
      601 tgccgcatta ttcaaagcga tattcagcat tactggaatc tgcgaattgt cgccagttcg
      661 ctatgtttaa gccccagcct gctcaaaaag aaattaaaaa acgaaaatac cagctatagc
      721 cagattgtca cagagtgtcg tatgcgttac gccgtacaga tgttattgat ggataacaaa
      781 aatatcactc aggtggcgca attatgtggc tatagcagca cgtcgtactt tatctctgtt
      841 tttaaggcgt tttacggcct gacaccgttg aattatctcg ccaaacagcg acaaaaagtg
      901 atgtggtgaa gggcaaagcg gaaacggata agacgggcat aaatgaagaa gaaatggcgc
      961 gccctgcagg attcgaacct gcggcccacg acttagaagg tcgttgctct atccaactga
     1021 gctaagggcg cgttgatacc gcaatgcggt gtaatcgcgt gaattatacg gtcaaccctt
     1081 gctgagtcaa tggcttttga tctggttgct gaacaagtga acgaccgcgt ctgattttct
     1141 gatttatttc gctatagtgg caaacaaacg cacaccgctg cgcgtctgaa tcaagaaaac
     1201 ccgtattttc atgtatcaaa gtacaattta ccgccctaac ggaaaattgt ccgctcctat
     1261 gagactggta actatgaaac caacgtcggt gatcattatg gatactcatc ctatcatcag
     1321 aatgtctatt gaagttctgt tgcaaaaaaa cagtgaattg cagattgtcc tgaaaacgga
     1381 tgattaccgc ataaccatcg attatctccg aacccgtcct gttgatttaa tcattatgga
     1441 tatagacttg cccggaacag acggttttac cttcctgaaa aggatcaaac aaatccagag
     1501 cacagtgaaa gtgttatttt tatcatcgaa atcagaatgc ttttatgctg gcagagcgat
     1561 acaagctggt gctaacggtt ttgtcagtaa atgcaatgat cagaatgata tttttcatgc
     1621 cgttcagatg atcctctccg gatacacgtt ttttcctagc gaaacgctta actatataaa
     1681 aagcaataaa tgtag
//
LOCUS       DAPHBJ010000346         1695 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.349, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000346 DAPHBJ010000000
VERSION     DAPHBJ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.349"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            97..312
                     /gene="essD"
                     /locus_tag="RHZ76_004522"
     CDS             97..312
                     /gene="essD"
                     /locus_tag="RHZ76_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HDV2815018.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            317..628
                     /locus_tag="RHZ76_004523"
     CDS             317..628
                     /locus_tag="RHZ76_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HDV2815019.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     L"
     gene            625..1158
                     /locus_tag="RHZ76_004524"
     CDS             625..1158
                     /locus_tag="RHZ76_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDV2815020.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            1155..1652
                     /locus_tag="RHZ76_004525"
     CDS             1155..1652
                     /locus_tag="RHZ76_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HDV2815021.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIYESLRD
                     KKHQM"
BASE COUNT          486 a          350 c          443 g          416 t
ORIGIN      
        1 tagcgccgtt gagaacaacg tgtgctgtca aaactacccc gtagactccg atcttttcaa
       61 acatattgca ccatccgtgt acatcggggt gaggatatga aatcaatgga taagttaaca
      121 acaggtgttg cctatggcac atcggcgggt aatgctggtt tctgggcatt gcagttactc
      181 gataaagtaa ctccgtcaca gtgggctgca atcggtgtgc tgggtagcct ggtttttggc
      241 ctgctgacgt atctgacaaa tctttatttc aagattaaag aagacaggcg taaggctgcg
      301 agaggagagt aatccaatga ctcaagacta tgaactggtt gtgaaaggag tccgtaattt
      361 tgagaataaa gttacggtaa ctgtagcctt acaggacaaa gaacgctttg acggtgaaat
      421 ttttgacctg gatgtcgcca tggaccgtgt tgaaggagct gcgctggagt tttatgaggc
      481 agcagccaga aggagcgtcc ggcaagtctt cctggaagta gcagaaaaat tgtcagaaaa
      541 agttgagtct tatctgcagc atcagtactc ctttaagatt gaaaatcctg ccaataagca
      601 cgagcgtcct catcataaat atctatgaac acaaaaatca gatacggcct gtcggctgcc
      661 gttctggcgc tgattggtgc tggcgcatct gctcctcaga tacttgacca gtttctggac
      721 gaaaaagaag gtaaccacac aatggcatac cgcgatggtt ctggcatatg gaccatctgt
      781 cggggtgcca cagtggtgga tggaaaaacc gtttttccca atatgaaact gtcgaaggaa
      841 aaatgcgacc aggtcaacgc cattgagcgt gataaggcgc tggcatgggt ggagcgcaat
      901 attaaagtac cactgaccga accacaaaaa gcgggtatcg cgtcattttg tccctataac
      961 attggccccg gtaagtgttt cccgtcgacg ttttataagc ggctgaatgc tggtgatcgt
     1021 aaaggtgcat gcgaagcgat tcgctggtgg attaaggatg gcggacgcga ttgccgcatt
     1081 cgttcaaata actgttacgg tcaggttatt cgtcgtgacc aggagagcgc attaacctgc
     1141 tgggggatag aacagtgaat cagatattca tggtgatttt tctcgtgttg tcaggattta
     1201 tcgtcggaaa tgtctggagc gaccgaggat ggcaaaaaaa atgggcggaa cgtgatgctg
     1261 ccgcattatc acaagaggta aatgctcaat ttgctgctcg aataattgaa caggggcgaa
     1321 ctatagcccg tgatgaggct gttaaagatg cgcaacagaa atctgctgaa atttctgcca
     1381 gggctgctta tctgtctgat agtgttaacc agttgcgtgc cgaagcaaaa aaatatgcca
     1441 tacgccttga cgcagcgaag cataccgcag atcttgccgc tgccgtcaga ggcaaaacaa
     1501 ccaaaaccgc cgaaggaatg ctcaccaaca tgctcggaga tattgcagca gaagctcagc
     1561 tttatgctga aattgctgac gaacgctaca tcgcaggagt gacttgtcaa cagatctatg
     1621 aatctttaag agataaaaag catcaaatgt agggtaatat taaatcggaa catttacatc
     1681 gcggaatgta aaatt
//
LOCUS       DAPHBJ010000347         1690 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.350, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000347 DAPHBJ010000000
VERSION     DAPHBJ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.350"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(106..1287)
                     /locus_tag="RHZ76_004526"
     CDS             complement(106..1287)
                     /locus_tag="RHZ76_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV2815022.1"
                     /translation="MNSKPVTRQFEDSDLHQELVTFEVPNNDLKELIFYFSHMKYHTA
                     KTYLQWLRSWNEWYQANAGKEGNEAWPASSLPVTQPPLLSYLDYLQGSLSHSSIKGCL
                     HALNSIHRKALDRPGIITSKVKSILASLEQAEAREQKVTRQATPFLVSDLKALIKAHG
                     TTQSVRKLRDLCIIWTGFETLLRSAELRRIRMQDLVLNEQTGSFTLTVYRTKSTVSTL
                     LTYHLTPHLTATLIRLMGMVKRDQQSHPKDYLFQAVNFQDSGYMPPGWGLRSKGNEIN
                     TLLKNHNMPYRPTRPPIGKNGKPIIVDDEGMLSKNTLLRAFEAFWDELHPQEAGTRCW
                     TGHSVRVGGAIELADAGYTHLQIMEMGNWSNAEMVSRYIRNIDAGKKAMTKFMREALD
                     E"
     gene            1502..>1690
                     /locus_tag="RHZ76_004527"
     CDS             1502..>1690
                     /locus_tag="RHZ76_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815023.1"
                     /translation="MSETKQAYLMKFRKCSSFDTLEKVFERLCEKNAGIASLEISGAY
                     DHRKAELTMKKLYDKVPAS"
BASE COUNT          451 a          385 c          407 g          447 t
ORIGIN      
        1 taagttggag tcattaccag agtttattgt caaataaaaa ggcagatttc tctgccttaa
       61 tacacaatat acatagttca aggtactata tataacgcag gggcgctact catccagcgc
      121 ctcacgcatg aacttcgtca tcgctttctt acctgcgtca atgttacgga tatagcggga
      181 aaccatttct gcattgctcc agttacccat ctccatgatc tgcaagtgcg tgtacccggc
      241 atcagcaagt tcgatggcgc cgccaaccct aacgctgtga ccagtccagc atcgggtgcc
      301 cgcttcctgc ggatgaagtt catcccaaaa cgcttcaaag gcccggagca atgtgttttt
      361 actgagcata ccctcatcat cgacaataat gggctttcca tttttcccga tcggggggcg
      421 ggtaggtcgg taaggcatat tatgattttt cagcagcgta ttgatctcat tccctttgct
      481 ccggagaccc catccagggg gcatatatcc gctgtcctga aagttaactg cctggaagag
      541 ataatccttc ggatggcttt gctggtccct tttgaccata cccatcagcc tgattagggt
      601 ggcagtgaga tgaggggtca gatgataggt aagcagggta ctgacagtgg attttgtccg
      661 gtacaccgtc agggtaaaac tgcctgtctg ttcattcagc acaaggtcct gcatccgaat
      721 gcgccgaagt tctgctgagc gcaacagtgt ttcaaaacct gtccagataa tgcaaaggtc
      781 tcggagtttg cgcaccgact gtgtcgtacc gtgggcttta atcaaagctt tcaggtcgga
      841 gactaggaat ggcgttgcct gacgagtcac tttttgctct cgggcttcag cttgttcgag
      901 gcttgcaaga atactcttga ccttggatgt gatgatccca gggcgatcga gtgctttcct
      961 atggatactg ttcagcgcgt gcagacaacc tttaatgctg ctgtgtgaca gcgagccctg
     1021 aagatagtcc agataggaca acaacggggg ttgagtcacc gggagagaac tggcaggcca
     1081 ggcttcgttt ccctcctttc ctgcattagc ttggtaccac tcgttccagg aacgcagcca
     1141 ttgaaggtaa gttttagccg tgtggtattt catatgagaa aagtaaaaaa tgagctcctt
     1201 aaggtcatta ttgggcacct caaacgtgac cagttcctga tggaggtctg agtcttcaaa
     1261 ctgtctggtt actggcttgc tgttcatggt ggtcacctga atacactgtt tttatttaca
     1321 gctattttag cactgaggta tccacttatg aacaggttca tagcaactaa aaaagagaga
     1381 gcgagtacaa caacgatccg gagataaaca tcaaatattt gtgaaaaaaa ttagattcta
     1441 gggacaaaac gacataagat tgggaaaccg tactttcgcg ggaaataaat taaggttgac
     1501 catgtccgaa actaagcagg cgtatttaat gaaatttcgt aagtgctctt ctttcgacac
     1561 actggaaaaa gtattcgaac gactctgtga aaaaaatgca ggtatagcat cactggaaat
     1621 ttccggggca tatgaccatc gaaaagctga actgacaatg aagaaactct acgataaagt
     1681 acccgcaagc
//
LOCUS       DAPHBJ010000348         1673 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.351, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000348 DAPHBJ010000000
VERSION     DAPHBJ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.351"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(30..1313)
                     /gene="ydfJ"
                     /locus_tag="RHZ76_004528"
                     /pseudo
     CDS             complement(30..1313)
                     /gene="ydfJ"
                     /locus_tag="RHZ76_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
BASE COUNT          486 a          425 c          335 g          427 t
ORIGIN      
        1 cctctccggt aagagaaggg attaagggtt tacagacttc tggaaggttg cgcagctctt
       61 acaacacgcg gttgatcttc cgcagcgtct tccagcgcac ttaaatcacg gtctttcacc
      121 tctggcattt tcagcgcaga gattaaacca atcactgaat atgccatgat cataatggcg
      181 atcggatacc aggattccgt catggtgcag aaaatacccg ccaggatagg accaaaaccg
      241 taagcgataa gaccaccaat ttctttagaa atagccatcc gggtaaagcg gtttttacag
      301 ccgaacattt ctgccatggt aatgttttcc agagcaaata atcccagcac cgcacagtta
      361 tgaatcacaa tcagtgcaac cataatggtg ctcggggcat agcttttatc tacaatgata
      421 gaaagcattg gccatgccag cacaatcgcg gaggtattca taataatata cgggatccgg
      481 cgaccaattt tatcggataa ccaaccaagg aacggaatgg tcataaagcc gagaatcgaa
      541 ctgatcatca atgcatctgt tggaattgct ttgttaaaca ataacgtctg cactaaatag
      601 cctgcaagga aagtctgaat taacccggag ttacccgcct gaccaaaacg cagccctgtt
      661 gccagccaga aggatttgct ctggaacatg ctaccagcag gtgcaggttt tgctgtcggt
      721 tggttactgt cgttaacctt ctcaaagacc gggctttctt tcagattcat acgcaaccag
      781 atagcaaaga ccatcacgac aacgctcgcc aggaacggta tacgccatcc ccacgccagc
      841 agttcctctt tactgagaat gaagaacata aaggcccaga ttgccgttgc gctcaaggtt
      901 ccgcagttag ttcccatagc cacaaatgag gagataattc cgcgcttacc ttttggcgca
      961 tattccgcca gcatcgtacc ggcaccggaa atttccgcac ctgcacccaa cccctgaata
     1021 atacgcaacg tcaccagcaa gatgggtgca aaaacaccaa tctgtgcata ggtcggtaac
     1081 acaccaatta aggtggtaca gatccccatc atggtgatgg taataaagag cactttttta
     1141 cgccctattc tgtcgcccat tttgccgaaa ataaatgctc cgacaatacg cgccacataa
     1201 cctgcaccgt aggttcccat tgccagaatt aacgccattg ccgttgatga ttcaggaaaa
     1261 aatatttcat gaaacactaa cgctgcgccg agcgaatata actggaaatc cataattcac
     1321 aggtgttttt tcccatcctg tggtttcctt ggcgttttct aggttttttc agatagttgc
     1381 attttttaaa aagcatccta agttcgatct cagtgtctat ctggggccta tttctgtccc
     1441 atatatgccc caaaaaaact ccccaacaga taagtagttt tttcatggat ttatgcgtaa
     1501 aatcaagaac ggctggaaat cattcaatac tcacactatc gaaaaattta ccagccaacc
     1561 gcagcacgtt cttgcataag gcgtgtctgc ggtttttcaa ctattcagat acatcactcc
     1621 catcacattc attcctccgc atcaaaggca tataggctat attaccttga tat
//
LOCUS       DAPHBJ010000349         1644 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.352, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000349 DAPHBJ010000000
VERSION     DAPHBJ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.352"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            45..739
                     /locus_tag="RHZ76_004529"
     CDS             join(45..291,291..739)
                     /locus_tag="RHZ76_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HDV2815024.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTAKTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDVQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(858..1598)
                     /locus_tag="RHZ76_004530"
     CDS             complement(858..1598)
                     /locus_tag="RHZ76_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005096525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HDV2815025.1"
                     /translation="MINLLPGEDIIYCGDNMNAPYGNRSADDIISLTKKMLTFLQSRN
                     VKLVAVACNTISSTLESEEYSGYAKSFPFPILSIIEPAVEDVIRQQYKNVGIIATEFT
                     IKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGNLNAPAILNDIKKHVNHLISLHPV
                     NEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAISIKNHLGQLNLLNSSNIGTLQIN
                     TSGSMEIYKTVLGELSITKPHTFSIRQF"
BASE COUNT          464 a          352 c          346 g          482 t
ORIGIN      
        1 attagttgaa tctgatgtat aatacgggct tttgaggtta tctcatgacc agcgttaata
       61 ttcattgtcc ccgttgtcag tcagctcagg tttaccgcca tggtcagaac cctaaaggcc
      121 gtgacagact tcgctgccgt gactgccacc gtgtgtttca gttcacttat acttatcaag
      181 cacgtaagcc gggtatgaaa gagctgataa ctgaaatggc ctttaatggt gccggggttc
      241 gcgataccgc caagacactg aaaattggta gtaacaccgt catccggact ttaaaaaact
      301 cgcgccaaag cgaataactt cttcgcctgt tgcccatgct gatgtggcgt ttatctgcga
      361 gcttgatgtg caatggagct acgttggcag taaagcccgg caacactggc tcgggtacgc
      421 gtacaacacc aaaacaggcg gtgtactggc ctacactttt ggcccccgaa ccgatcaaac
      481 gtgccgggag ctactggcac tgcttacacc cttcaacatc ggcatgctga ccagcgatga
      541 ctggggcagc tatggccggg aggtgccgaa gaataagcat ctgaccggca aaatattcac
      601 ccaacgcatt gagcgtaata atctgacgct acgcacccgc attaagcggt tgggtcgtaa
      661 aacaatctgc ttctcacgct cagttgagat ccacgaaaaa gtgatcggag catttatcga
      721 aaaacacatg ttctactaat tggatgcact acccagtatg taactgggtt tgtccataaa
      781 atgcacctta ctcagttggt gtccaacttt tggggtgcag ttcagaagga cggggtttta
      841 cggtgcaccg gataaattta aaactgcctg atactgaatg tatgaggttt cgtaatgctc
      901 aattccccta gtaccgtttt gtagatctcc atacttccac tagtatttat ttgcaaagtt
      961 cctatatttg aggagtttaa taaatttagc tgtcccaagt gatttttaat agagatagcc
     1021 tgatcatgtg caggattaat aaattttata tccggtgcaa ctgcttcaaa aaaattctga
     1081 acaattggat agtgagtaca gccgagaata atttcattta ctggatggag ggaaattaaa
     1141 tggttaacat gttttttgat gtcattcaag attgcaggag cattaagatt accttcctca
     1201 attaacattg caagattttt gcttggttct ccaaatacat ttattgttga gttcaatttt
     1261 tttattagct ctttgtgaca ccccgtttta atagtaaact cggtagcgat aataccaaca
     1321 tttttatatt gctggcgaat cacatcttct actgcaggtt caatgatgga taagatagga
     1381 aacggaaagc tcttagcata tccgctgtat tcttcggatt ccaaagttga ggatatagta
     1441 ttacatgcga cagcaactag tttgacattc cttgactgta aaaaagtcag cattttttta
     1501 gtcaaactga tgatatcatc ggcagaacgg ttgccataag gtgcattcat attatctcca
     1561 caatagataa tatcttcacc aggaagcaaa ttaattattt cctttaccga tgtcaatcca
     1621 cctacacctg agtctattac acca
//
LOCUS       DAPHBJ010000350         1632 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.353, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000350 DAPHBJ010000000
VERSION     DAPHBJ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.353"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..539
                     /locus_tag="RHZ76_004531"
     CDS             <1..539
                     /locus_tag="RHZ76_004531"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV2815026.1"
                     /translation="QYLPDWRPSKSYRESVSASKVLGGGALLELSHELDYAQWLFGEL
                     KLVNSVLRTSSELEMDVESLADIIVINSAGSLINIHLDFLQKKPWRQCHIIGSKGRIV
                     WDLIRNEIIHHTRQSSDIIFSDPGWDKNGMYTDMLLDFIAEISGSDNNCVTLESSINI
                     VSLIDKIKETSQCLGEVQ"
     gene            536..1231
                     /locus_tag="RHZ76_004532"
     CDS             536..1231
                     /locus_tag="RHZ76_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010431994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDV2815027.1"
                     /translation="MKINAFIFARGGSKGLPGKNIKPLAGKPLLQYSIETAKQSPSIS
                     SIYVSTDDDDIALVAENCGATVIRRPAELAGDTSPEWLAWRHAIEWVQKEVGDFDGFV
                     SLPTTSPLRSVDDVECAIAKRVESGADICISVTPASRSPYFNMVKFHESGYVRLVNEP
                     EGKVLRRQDSPDVFDITTVVYATTPKFVLNNYGLFSGKVASIIVPKERAVDIDDIFDF
                     YMAEILLKELNRG"
     gene            1224..>1632
                     /locus_tag="RHZ76_004533"
     CDS             1224..>1632
                     /locus_tag="RHZ76_004533"
                     /inference="COORDINATES: protein motif:HMM:NF013530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDV2815028.1"
                     /translation="MANILKGKKILIAGAGGLLGTHLVKKVIDEGGYVIAGDFDLVST
                     QNKLNELGITTGYELHQLDVTSLESVQEILAIAPDLDGAINTTYPRNKTYGAHFYDVT
                     LESFNENLSLHLGSSFLFSQQCAAYFKKNQRSFS"
BASE COUNT          488 a          284 c          366 g          494 t
ORIGIN      
        1 gtcaatatct gccggattgg agaccctcaa aatcatatcg tgaaagtgta tcagcaagta
       61 aagttttggg tggcggagcg ctactcgaat taagtcatga gctggattat gcgcaatggc
      121 tatttggtga actgaaactt gtgaattcag tgctacggac ttcgtcagaa cttgaaatgg
      181 atgtcgaatc tttagctgat attatagtga taaattccgc cggttcgctt attaatatcc
      241 atcttgattt ccttcaaaag aaaccatggc gacaatgtca tattattggc agtaagggac
      301 gaattgtctg ggatctcatc cgcaatgaaa tcattcatca tactcgacag agtagcgata
      361 ttatctttag tgatcctggc tgggataaaa atggtatgta tacagatatg ctgctcgact
      421 tcattgcgga aatatccggg agtgataaca actgtgtgac attggagtca tcaataaata
      481 ttgttagctt aattgataag ataaaagaaa caagtcaatg ccttggagaa gttcaatgaa
      541 aataaacgct tttatatttg cacgtggggg atcaaaagga ttacctggta aaaatattaa
      601 acctctagca ggcaaacctc tcctgcaata ctctattgag actgccaaac agagtccttc
      661 gatttcctcc atatatgttt caaccgacga tgatgatatt gctctcgttg cagaaaattg
      721 tggtgctact gttattcgta gaccggcgga gcttgccgga gacactagtc cagaatggtt
      781 ggcatggcgt cacgcaattg agtgggttca gaaggaagtg ggggatttcg atggatttgt
      841 aagtttaccg actacaagcc ctttgcgaag tgtcgatgat gtcgaatgtg ctattgctaa
      901 aagagtcgag tctggtgctg acatatgcat ttcagtcact ccggcgagca gaagtcctta
      961 ttttaatatg gtaaaatttc acgaaagtgg ttatgtgagg cttgtcaatg aaccggaagg
     1021 gaaagtgctc agacggcagg attcaccaga cgtatttgat attactactg ttgtgtacgc
     1081 gacaacgcca aaatttgttt tgaataatta tgggctattc tcaggcaaag ttgcaagcat
     1141 tattgtgcct aaagaacgtg ccgttgatat agatgatatt tttgattttt acatggcgga
     1201 aattttactt aaggaattga atcgtggcta atatacttaa agggaaaaaa atccttattg
     1261 ctggtgccgg tggattgcta ggcactcatc ttgttaaaaa agtcatagat gaaggtggtt
     1321 atgttattgc cggtgatttt gacttagtgt caacacagaa caaattaaat gagctgggta
     1381 tcacaactgg atatgaatta catcaacttg atgtaacaag tcttgaatct gttcaggaaa
     1441 tactagccat tgcacctgat ctggatggtg ctattaatac gacttatcct cgaaataaaa
     1501 cttatggtgc ccacttttac gatgtaacgc tggaaagttt caacgaaaat ctttcgttac
     1561 atcttggaag ttcatttctt ttctcacaac agtgtgcagc atatttcaaa aaaaatcagc
     1621 gttcattttc ac
//
LOCUS       DAPHBJ010000351         1596 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.354, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000351 DAPHBJ010000000
VERSION     DAPHBJ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.354"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            30..344
                     /locus_tag="RHZ76_004534"
     CDS             30..344
                     /locus_tag="RHZ76_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncN plasmid KikA protein"
                     /protein_id="HDV2815029.1"
                     /translation="MKKLLIPLIAAGSLLYLPASHAEDPCKVIMCMAGKLTGDSGGSE
                     CNSAEAAFFNIVKKNKHGFLPNHTKDARKAFLNECPDNGEGGSNQSMISQIISKYGKV
                     RL"
     gene            380..691
                     /locus_tag="RHZ76_004535"
     CDS             380..691
                     /locus_tag="RHZ76_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001452736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815030.1"
                     /translation="MKKIILSALACTALLTGCVSQDKGNAMQSQLNNQQRQINELSVR
                     LQSAESRLSKQEEKLRNELLQSSGYCYLNGARYSTGTVLYGRICQNQSGSASWQVYSR
                     R"
     gene            747..1388
                     /locus_tag="RHZ76_004536"
     CDS             747..1388
                     /locus_tag="RHZ76_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HDV2815031.1"
                     /translation="MIPFPTTENLILWACSAIVLLAVVFFRRSVRHRRHKRKQQSARR
                     VLERIKTLPGFPQKINYLRKIDPFVFEELLLEGFEAHGFRTIRNKRYTGDGGIDGQVI
                     IGKYRYLIQAKRYRGHIALQHVQEFEKLLKRHNCRGLFCHTGKTGAGSKSVSIASERM
                     EIISGQRLIDLLTPGSSFTIATAPQTMMKRTAATLETSTIVKDAGKENRYHES"
     gene            1393..1590
                     /locus_tag="RHZ76_004537"
     CDS             1393..1590
                     /locus_tag="RHZ76_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815032.1"
                     /translation="MKSVTIEAKTFAEMLGITEGELIFAIKKTGTFKNKTIPQPHEPH
                     KSNNRFLYSDVMRFIESLKDK"
BASE COUNT          496 a          365 c          369 g          366 t
ORIGIN      
        1 atatctgaac aaactgttaa ggacaaaaaa tgaagaaact cttaatacct ctgatagcag
       61 ctggtagtct gctttatctt cctgccagcc atgctgaaga tccctgcaaa gttattatgt
      121 gcatggcggg caagctcacc ggcgatagcg gcggaagcga gtgtaacagt gctgaagctg
      181 ctttcttcaa tatcgttaaa aagaacaagc acggcttttt acccaaccac acgaaggatg
      241 ctaggaaggc ttttcttaat gaatgcccgg ataatggcga aggtggaagt aaccagtcga
      301 tgataagcca gatcataagt aaatacggga aagttcgctt ataggcgaac caggaataat
      361 ctcaatttaa ggagccaacg tgaaaaaaat aatattaagt gcattagcct gtacagctct
      421 cttaactggt tgcgtcagcc aggataaagg caatgcgatg cagagccagc tgaataacca
      481 gcaacgccag attaacgaat tatccgttcg tttgcagtct gcggagtccc ggctatcaaa
      541 gcaggaagaa aagctgcgca acgaactgct gcaatccagc ggctattgct atctgaatgg
      601 cgcccgctac tcgaccggca ccgtacttta cgggcggatt tgccagaatc agtcaggcag
      661 cgcttcgtgg caggtataca gccgtcgcta aacatcaggg cggtttaacc gcccttttct
      721 ttaccctcag aaagcccact ataaccatga tccccttccc cactacagaa aaccttattc
      781 tatgggcttg cagcgccatc gtactgcttg ccgttgtatt cttccggcgt tcagtacggc
      841 acaggcgaca caaaaggaag cagcaaagtg cgcggcgggt gctggagcgc ataaagacgt
      901 tgccgggctt cccacaaaaa attaactacc tgagaaaaat tgatcctttt gtgtttgaag
      961 aactgttgct ggaaggattt gaagcgcatg gcttcagaac catcagaaac aaacgctata
     1021 ccggcgatgg aggcattgac ggccaggtaa taataggaaa atatcgctat cttattcagg
     1081 ctaaacgcta tcgcggccat attgctttac agcacgtaca ggagttcgag aagttgctta
     1141 aacgtcataa ctgtcgcggt ctgttttgcc ataccgggaa aaccggcgca ggttcaaaat
     1201 ctgtcagtat tgccagtgaa cggatggaga ttatcagcgg ccagcgcctg atagatttgc
     1261 tcacgcccgg tagctccttc actatcgcaa ccgccccgca gacgatgatg aagcgtaccg
     1321 cagcaacgct agaaacgagc accattgtta aagatgccgg taaagaaaat cgataccatg
     1381 agagttaatt aaatgaagtc agtaactata gaagcaaaaa catttgctga aatgttagga
     1441 ataacagaag gtgagttaat ctttgccatt aagaaaactg gcacattcaa aaacaagaca
     1501 atcccacaac ctcatgagcc acataaatca aataatagat ttttatattc agacgtaatg
     1561 aggtttatag aatcactaaa agacaaatag aaccgg
//
LOCUS       DAPHBJ010000352         1521 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.355, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000352 DAPHBJ010000000
VERSION     DAPHBJ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.355"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            12..536
                     /locus_tag="RHZ76_004538"
     CDS             12..536
                     /locus_tag="RHZ76_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815033.1"
                     /translation="MNIENQDLFNKFAAVISSRIVEQPSSCYYLHDSEIDFTILKHSI
                     IDKDKNLLYVIRPSGTCLLRCDKYFFPNYYLTSRGDYKAFKYVHFNLATREAEEITWQ
                     QAFEILSKPGRPPLRGSLGKFDYLKLVIDDLRARGYADFLPAYNLDGLRHFAVKDERP
                     SLVSYIDNVMALCA"
     gene            614..1492
                     /locus_tag="RHZ76_004539"
     CDS             614..1492
                     /locus_tag="RHZ76_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815034.1"
                     /translation="MKLATDLVNAVLIKNGIVPTSINELPVTSLVKPLTQVHLSLYLK
                     TTHLSQFVPDYVESLKDCPVSYDIGLELQQCINSAELLCEDMESLEFISVWIELYTCN
                     SKQQQFEKSNFSFRTEDIELAKMLSCSHEILISQAACFWLLYFEDFMKYVRNRLCALL
                     RQVLILIAGLRNVYLQRNEDQLIKRVKHGNEMLEVTLKAVALCSIERYLKSYASLRTE
                     AAASGFIKCIQSYISTGWRHGYIVCQYSKNGRSLGKSLPLFIKETSEHNIQKRVNRLI
                     SSTISGRNKDLGLYDF"
BASE COUNT          437 a          281 c          317 g          486 t
ORIGIN      
        1 agagcattgt tatgaatatt gaaaatcaag atttatttaa taagttcgct gctgtgattt
       61 cttctcgtat agttgagcaa ccatcatcgt gttattacct tcatgatagt gaaattgact
      121 ttacgatttt aaagcacagc ataatagata aagacaaaaa cctgttgtac gttattcgtc
      181 cttcaggaac ctgtttgttg cgttgtgata aatacttctt tccgaattac tatcttacaa
      241 gtcgtggaga ttataaagca tttaagtatg tgcattttaa tcttgcaaca cgagaagcgg
      301 aagaaatcac ctggcagcaa gcttttgaaa ttttgtccaa gccgggaaga cctccattaa
      361 gaggttcact tggtaaattt gattatttaa aacttgttat tgatgatctt cgtgcgcgag
      421 ggtatgccga tttccttcca gcatacaacc ttgatggcct tcgtcacttt gcagtaaagg
      481 atgagcgacc atcacttgtc agttatatcg acaatgtcat ggcactttgc gcataaagat
      541 aacacatatt tttactacat ccctgatggc acctcccatc ggggtggccc tcagagttga
      601 atctggaggt cttatgaaat tagctacaga tctggtcaat gcggttctga taaagaatgg
      661 cattgtacca acttccatta atgagttgcc tgtaacgtcg ctggtaaagc cattgacaca
      721 agtgcatctc agcctgtatc tgaaaacaac gcatttatct cagttcgtcc ctgactacgt
      781 tgagtctctg aaagattgtc cggtttcgta tgatatcggc cttgaacttc agcagtgtat
      841 caacagtgct gaattgctct gtgaagacat ggagtcactt gagtttatct ctgtatggat
      901 agagctctat acgtgtaatt caaagcaaca gcagtttgag aaaagtaact tttcttttcg
      961 aactgaagat atcgagctgg ctaagatgct gtcttgctcg catgaaatct taataagtca
     1021 ggcggcttgt ttctggcttt tgtattttga agatttcatg aaatatgttc gtaataggct
     1081 ctgtgcatta ctcagacagg ttttaatttt gattgcagga ctgcgaaatg tatacttgca
     1141 acggaatgaa gatcagttga ttaagagagt taagcacggg aatgaaatgc tggaggtgac
     1201 tcttaaagct gttgcactat gttcaataga aaggtatctg aaatcatatg cctctttacg
     1261 aacagaagcg gcggcttctg ggtttatcaa atgcattcag tcttatatca gtactggttg
     1321 gagacatggc tatatagtct gtcaatattc aaaaaatgga cggtctcttg gtaagtcatt
     1381 accacttttt ataaaagaga catctgagca taatatacag aaacgtgtca atcgccttat
     1441 atcttccacg atttcggggc gaaataaaga tttaggtctt tacgatttct gattaacatt
     1501 acccatccgg ggattacatc c
//
LOCUS       DAPHBJ010000353         1514 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.356, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000353 DAPHBJ010000000
VERSION     DAPHBJ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.356"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..1514
                     /locus_tag="RHZ76_004540"
                     /pseudo
     CDS             1..1514
                     /locus_tag="RHZ76_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          346 a          482 c          457 g          229 t
ORIGIN      
        1 ccggacaaaa acgcccgcgc cggtggggag cctcggcccc ggctggaaaa tgcctgcgga
       61 tatccgctta cagctgcgcg ataacacact gatactcagt gataacggcg gcagaagcct
      121 gtattttgag cacctgtttc ccggtgagga cggttacagc cgcagcgagt cactgtggct
      181 ggtgcgcggc ggcgtcctga gactggatga aggtcaccgg ctggccgcac tctggcaggc
      241 gctgccggaa gaactccgct taagtccgca tcgttatctg gcgacaaacc ggctttgacg
      301 gccgcacaca gcgttatcac cacgacctga ccggcaaact tatccgcagc gaggatgagg
      361 ggctggtcac ccactggcac tatgacgaag cagaccgcct cacgcaccgc accgtgaatg
      421 gcgaaaccgc agagcggtgg cagtatgacg aacgcggctg gctgacagac atcagccata
      481 tcagcgaagg gcaccgggtg gcggtgtatt acgggtatga tgagaaaggc cgcctgaccg
      541 gtgagcgtca gacggtgcat cacccggaga cggaagcact gctctggcag catgagacca
      601 gacatgcgta caacgcgcag ggactggcga accgctgtat accggacagc ctgcccgccg
      661 tggaatggct ggcctacggc agcggttacc tggcaggcat gaagctcggc gacacaccgc
      721 tggtggattt cacccgcgac cgcctgcacc gggaaacgct gcgcagcttc ggccgttatg
      781 aactcaccac cgcttacacc cctgccgggc agttacagcg tcagcacctg aacagcctgc
      841 agtatgaccg cgattacacc tggaacgaca acggcgaact catccgcatc agcagcccgc
      901 gccagacccg gagttacagc tacagcacca ccggcaggct gaccggcgtt cacaccaccg
      961 cagcgaatct ggatatccgc atcccgtatg ccacagaccc ggcaggtaac cgcctgcccg
     1021 acccggagct gcacccggac agcaccctca gcatgtggcc ggataaccgt atcgcccgtg
     1081 acgcgcacta tctttaccgg tatgaccgtc acggcaggct gacagagaaa accgacctca
     1141 tcccggaagg ggttatccgc acggatgatg agcgcaccca ccagtaccat tacgacagtc
     1201 agcaccggct ggtgcactac acgcggacac aatatgcaga gccgctggtc gaaagtcgct
     1261 atctttacga cccgctgggc cgcagggtgg caaaacgggt atggcgacgt gaacgggacc
     1321 tgacgggctg gatgtcgctg tcacggaaac cggaagtgac ctggtacggc tgggacggcg
     1381 accggctgac cacgatacag aacgacagaa cccgcatcca gacgatttat cagccgggga
     1441 gcttcacgcc actcatcaga gttgaaaccg ccaccggtga gctggcgaaa acgcagcgcc
     1501 gcagcctggc ggat
//
LOCUS       DAPHBJ010000354         1508 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.357, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000354 DAPHBJ010000000
VERSION     DAPHBJ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.357"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(176..517)
                     /locus_tag="RHZ76_004541"
                     /pseudo
     CDS             complement(176..517)
                     /locus_tag="RHZ76_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414786.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
     gene            complement(609..1472)
                     /locus_tag="RHZ76_004542"
     CDS             complement(609..1472)
                     /locus_tag="RHZ76_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDV2815035.1"
                     /translation="MNNAQGLQPLNHSLSGLPQWVSERILQQINQLTHYEPVIGIMGK
                     TGAGKSSLCNALFVGDVSPVSDVAACTREPLRFRLQVGERFMTIVDLPGVGESGARDS
                     EYAALYREQLPRLDLVLWLLKADDRALAVDEHFYHQVIGEAYRHKVLFIISQSDKTEP
                     TSGSGQLSTEQKQNISRKICLLHELFQPVHPVCAVSVRLQWGLKVMAERMIKCLPREA
                     SSPVVAQLQSPFRTTAVQEKARDDFGETVGAVLDTVSAFPLIPAPVRAVIQAVRNTVV
                     SVARTVWDFFF"
BASE COUNT          373 a          420 c          372 g          343 t
ORIGIN      
        1 tgtttcatct ttataaatgc ccccttttgt gcccccacct tgtttttcat aggactcgat
       61 gagcctttca tcgagtcgcc aatatcacca atttcgttac agaccgtgca aatcttctcg
      121 actcctggag ctctcctcac tgccgtgatc ggctaagaac aatattactt acttcccgat
      181 acgagctggt gccatcatgg gagttgagca ggataatttc cggcacctgc ttaccggtaa
      241 tctgcccctc cctacgcaga cgcagcatat gcttcgtatg ttcacgacgg ccaggatcac
      301 gtacccgggt ctgacaggca aagaatggct ggaagccttc ccgctgcagg ctgtccagca
      361 gggagatggt tggaatatag gtataacgtt cgctgcggga tttgtgtttg tcctcgctga
      421 atacgctggg gaccactcga aacaactctt cacgggttaa cggacggtca cggcggataa
      481 gatttgctgc accaaagcgc gaggccagac gggtcataag cagactcctc ataatggaaa
      541 aacaaataaa agaaatcccc gtcgcacagg agacagggtt agggaaataa cagggatggg
      601 ttaaatactt agaagaagaa atcccacaca gtacgggcaa cggataccac ggtgttacgg
      661 acggcctgta ttacggcacg taccggagcc ggtatgagtg ggaaagcact taccgtgtcc
      721 agtaccgcgc cgaccgtttc accaaaatcg tcacgcgctt tctcctggac tgctgttgtg
      781 cgaaagggag actgcagttg cgccaccacg gggctgctgg cctcacgtgg caggcacttt
      841 atcatccgct cggccatcac cttcagtccc cactgcagtc ggaccgacac cgcacatacc
      901 ggatgcacgg gttggaacag ttcatgcagc agacagattt tgcggctgat attctgcttc
      961 tgctccgtgg acaattgacc actaccgctg gtgggttcag tcttatctga ctggctgata
     1021 ataaacagta ccttatgccg gtaagcctcc ccaatcacct gatgataaaa gtgctcatcc
     1081 actgctagcg cccggtcatc agccttaagc agccacaaca ccaggtcaag tcggggaagt
     1141 tgttcacggt acagcgcagc atactcgcta tcgcgagcgc cgctttcgcc cacgccgggc
     1201 aggtccacga ttgtcataaa ccgctcaccg acttgcagac gaaatcgcaa aggttcacgg
     1261 gtacaggctg ccacatcgct gaccggcgat acgtcgccga caaacaaggc attgcaaaga
     1321 ctactctttc ccgcccctgt tttacccata atgccgatca ctggctcgta atgggttaac
     1381 tgatttatct gctgcaatat acgttcagac acccattgcg gtaggccgga aagcgaatgg
     1441 tttagtggct gtaagccctg tgcattattc atgactactc ctgcaataaa taaaactaaa
     1501 acggcaga
//
LOCUS       DAPHBJ010000355         1500 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.358, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000355 DAPHBJ010000000
VERSION     DAPHBJ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.358"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            41..1447
                     /gene="gndA"
                     /locus_tag="RHZ76_004543"
     CDS             41..1447
                     /gene="gndA"
                     /locus_tag="RHZ76_004543"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDV2815036.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDSNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIVDDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT          385 a          364 c          384 g          367 t
ORIGIN      
        1 tcacaggtcg tgtggagacc gcatctgaca ggagtaaaca atgtcaaagc aacagatcgg
       61 cgtagtcggt atggcagtga tggggcgcaa ccttgcgctc aacatcgaaa gccgtggtta
      121 taccgtctct attttcaacc gttcccgtga aaagacggaa gaagtgattg ccgaaaatcc
      181 aggcaagaaa ctggttcctt actatacggt gaaagagttt gttgaatctc tggaaacgcc
      241 tcgtcgcatc ctgttaatgg tgaaagcagg tgcaggcacg gatgctgcta ttgattccct
      301 taagccatac ctcgataaag gtgacatcat cattgatggt ggtaatacct tcttccagga
      361 caccattcgt cgtaaccgtg agctttctgc agaaggcttt aacttcatcg gtaccggtgt
      421 ttctggcggt gaagaaggtg cgctgaaagg tccttccatt atgcctggcg gacagaaaga
      481 agcctatgaa ctggttgcac cgatcctgac caaaatcgcc gcagtggctg aagacggtga
      541 gccatgcgtt acctatattg gtgccgatgg cgcaggtcac tatgtgaaga tggttcacaa
      601 cggtattgaa tacggcgata tgcagctgat tgctgaagcc tattctctgc ttaaaggtgg
      661 cctgaacctt tccaacgaag agttggcgca gacctttacc gagtggaata acggtgaact
      721 gagtagctac ctgatcgaca tcaccaaaga catcttcacc aaaaaagatg aagacagtaa
      781 ctacctggtt gatgtgatcc tggatgaagc agcaaacaaa ggtaccggta aatggaccag
      841 ccaaagcgcg ctggatctcg gtgaaccgct gtcgctcatt accgagtctg tgtttgcacg
      901 atacatctct tctctgaaag agcagcgcgt tgcggcgtcc aaagtactgt ctggtccgca
      961 agcacagcca gcaggcgaca aggctgagtt catcgaaaaa gttcgccgtg cgctgtatct
     1021 tggcaaaatc gtttcttacg cccaaggctt ctctcagctc cgtgctgcgt ctgaagagta
     1081 caactgggat ctgaactacg gcgaaatcgc gaagattttc cgtgctggct gcatcatccg
     1141 tgcgcagttc ctgcagaaaa tcactgatgc atatgccgaa aatccgcaga tcgctaacct
     1201 gctgctggct ccgtacttta aacaaattgt cgatgactac cagcaggcgc tgcgtgatgt
     1261 cgttgcttat gcagtacaaa acggtattcc ggttccgacc ttcgccgctg cggttgccta
     1321 ttacgatagc taccgtgccg ctgttctgcc tgcgaacctg atccaggcac agcgtgacta
     1381 tttcggtgca catacttata agcgcattga taaagaaggt gtgttccata ctgaatggct
     1441 ggattaatct gatttaacca acaataaaat tgaggcccgg cgtatattgc accgggcttt
//
LOCUS       DAPHBJ010000356         1473 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.359, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000356 DAPHBJ010000000
VERSION     DAPHBJ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.359"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            685..933
                     /locus_tag="RHZ76_004544"
                     /pseudo
     CDS             685..933
                     /locus_tag="RHZ76_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            998..>1473
                     /locus_tag="RHZ76_004545"
     CDS             998..>1473
                     /locus_tag="RHZ76_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HDV2815037.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPR"
BASE COUNT          442 a          329 c          355 g          347 t
ORIGIN      
        1 aggtttttaa agaaccttac ctagatcaaa ccaaccccca taaactcgtt cagagtgccg
       61 tagaaatctc agcaaaaaaa atcacgccca atcccctaaa acccgcacct tacctatggt
      121 cagagctttc agcatcaaga ttaacgcaat gctaaagcac ctcatatttt gtaatgaaaa
      181 ttttatcgtt ccagaaaccg cagtcgggtg cggtgtagtg ccgtagcgtc acgtgacagc
      241 acagatcctc ttgtggtgtt gcacggtagc gcagtgatat tcgggcccga cagtcattag
      301 tagcatgaca aagggatgtg aaaaccacat gggcctcaat gaaccgtgct gtggggaact
      361 atccgcgctg attaagctgc gaccaaaggc ccgcttcttg ttcaaagcag ccctttgaac
      421 cttaacggtg gcagctcagt gccatgaatc acctgatatt taaaagctca aggattacta
      481 ggatagatat gtggttgaga tatcgatctg gttctgatcg atttttccgc taatgtgtaa
      541 caggtgaaga tcatagtcta aacgtcggat gttaaaaccg ggctgagctt cgttccgaac
      601 aagaagcttt tggctcaaaa gtgaatcgcc acaggtttaa cagacacctc agcgtcattt
      661 aagatgactt aaagagaggt gcccatgagc ggtaagcgtt atcccgaaga gtttaaaatt
      721 gaagcagtca aacaggttgt tgatcgcggt tattctgttg ccagcgttgc aacacgtctc
      781 gatatcacca cccacagcct ttactcctgg ataaagaagt acggtccgga ttcttccatt
      841 aataaagaac agtcagatgc tcaggccgag atccgccgtc tccagaaaga gctgaagcgg
      901 gttaccgacg aacgggacat attaaaaaaa gccgctgtag attcaattgg tcaacgcaac
      961 agttatgtga aaacatgggg ttgcggaggt tttttgaatg agacgaacat ttacagcaga
     1021 ggaaaaagcc tctgtttttg aactatggaa gaacggaaca ggcttcagtg aaatagcgaa
     1081 tatcctgggt tcaaaacccg gaacgatctt cactatgtta agggatactg gcggcataaa
     1141 accccatgag cgtaagcggg ctgtagctca cctgacactg tctgagcgcg aggagatacg
     1201 agctggtttg tcagccaaaa tgagcattcg tgcgatagct actgcgctga atcgcagtcc
     1261 ttcgacgatc tcacgtgaag ttcagcgtaa tcggggcaga cgctattaca aagctgttga
     1321 tgctaataac cgagccaaca gaatggcgaa aaggccaaaa ccgtgcttac tggatcaaaa
     1381 tttaccattg cgaaagcttg ttctggaaaa gctggagatg aaatggtctc cagagcaaat
     1441 atcaggatgg ttaaggcgaa caaaaccacg tca
//
LOCUS       DAPHBJ010000357         1472 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.360, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000357 DAPHBJ010000000
VERSION     DAPHBJ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.360"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1440
                     /locus_tag="RHZ76_004546"
     CDS             <1..1440
                     /locus_tag="RHZ76_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDV2815038.1"
                     /translation="IEHLRSQIEKLRRMMFGTRSEKLRREVAQAEALLKQREQASDRY
                     SGREDDPQVPRQLRQSRHRRPLPEHLPREILRLEPEETCCPACGGEMAYLSEVSAEQL
                     ELVASALKVIRTVRVKKACTKCDGIVEAPAPSRPVERGIAGPGLLARVLTAKYCEHLP
                     LYRQTEILARQGVELSRALLSNWVDTCCRLMAPLDEALYHYVMNCRKLHTDDTPVPVL
                     APGRKKTKTGRIWTYVRDDRNAGSSDPPAAWFAYSPDRQGKHPELHLRSFRGVLQADA
                     FAGYDRLFSTSREGGVLTEAACWAHARRKIHDVYISSQSATAEEALKRIGELYAIEAE
                     IRGMPETERLAARQSRSAPLLASLHEWMVEKSATLSKKSRLGEAFTYALNQWDALCYY
                     CRDGLAEPDNNAAERALRAVCLGKKNYIFFGSDHGGERGALLYGLIGTCRLNGIDPEA
                     YLRHILSVLPEWPSNRVGELLPWNVAIPA"
BASE COUNT          316 a          423 c          470 g          263 t
ORIGIN      
        1 atagaacacc tgaggtctca gatagaaaaa ctgcgccgta tgatgttcgg cacccgttct
       61 gaaaaactgc gccgcgaggt ggcacaggcc gaagcgctgc tgaaacagcg cgaacaggcg
      121 agcgatcgtt acagtgggcg ggaagacgac ccgcaggttc cccgccagct ccgtcagtcc
      181 cggcaccgtc gtccgcttcc ggaacatctt ccccgtgaga tactccggct ggagccggaa
      241 gaaacctgct gcccggcatg tggcggtgaa atggcgtacc tcagcgaagt cagcgcggag
      301 cagctggagc tggttgccag cgccctgaaa gtgatccgca cagtgcgggt aaaaaaagcc
      361 tgcacaaaat gtgacggtat cgtggaagct cccgcgccgt cacgtcctgt cgagcggggt
      421 atcgccggac cgggtctgct ggcccgtgtg ttaacagcca aatactgtga acatctgccg
      481 ctgtaccgcc agacggaaat ccttgcccgc cagggggtgg agctgagccg cgcactgctc
      541 tccaactggg tggatacgtg ctgccgttta atggcgccgc tggacgaagc cctttatcac
      601 tacgtgatga actgccgcaa actgcataca gatgatactc cagtgccggt gctggcgccg
      661 ggcaggaaaa agacgaaaac cgggcgtatc tggacgtatg tccgggatga ccggaatgcc
      721 ggttcgtcag atcctccggc agcgtggttc gcgtactcac cggaccggca gggaaaacac
      781 ccggagctgc accttcgctc cttccgtggc gtcctgcagg cagatgcctt cgcggggtat
      841 gaccggctgt tcagtacatc gcgagaaggc ggcgtgctga cagaggcggc atgctgggcg
      901 catgcccgcc gaaaaatcca cgacgtgtac atcagcagcc agagcgccac ggcggaggaa
      961 gcgctgaaac gcatcggcga actgtacgcc atcgaagcgg aaatacgcgg catgccggag
     1021 acagagcgtc tggcggccag acagtcgcgg agtgcgccgc tgctggcgtc actgcatgag
     1081 tggatggtgg agaaaagtgc gacactgtcg aaaaaatccc ggctgggaga ggcgttcacg
     1141 tatgccctga accagtggga tgccctgtgt tactactgcc gtgacggtct ggcggaaccc
     1201 gacaataacg ccgcggagcg ggcgcttcgc gccgtctgcc tgggaaagaa aaactacatc
     1261 ttcttcggga gcgatcatgg tggcgaacgt ggtgcactgc tgtacggact gatcggaacg
     1321 tgccgtctga acggtatcga tccagaagcg tatctgcgcc acatcctgag cgttctgcct
     1381 gagtggcctt ccaacagggt tggtgaactt ctgccgtgga acgtggctat acctgcctga
     1441 ccagtgtcaa tacggcgtcc ggttaacgct ta
//
LOCUS       DAPHBJ010000358         1460 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.361, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000358 DAPHBJ010000000
VERSION     DAPHBJ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.361"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            126..1195
                     /gene="ompN"
                     /locus_tag="RHZ76_004547"
                     /pseudo
     CDS             126..1195
                     /gene="ompN"
                     /locus_tag="RHZ76_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            complement(1211..>1460)
                     /locus_tag="RHZ76_004548"
     CDS             complement(1211..>1460)
                     /locus_tag="RHZ76_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HDV2815039.1"
                     /translation="LMSLLSPFDVVIWMTDGWPLYESRLKGKLHVISKRYTQRIERHN
                     LNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          401 a          331 c          347 g          381 t
ORIGIN      
        1 tgattaaggt tttacttaag gcgtaacaaa tgatatttat cggctaactg aacttctcct
       61 ttaggatgtt ttcactcccc gaaatgggga taaaaggcaa ataaaataac taaaggattt
      121 attcaatgaa aagcaaagta ctggcacttt taattcctgc cctgctcgcc gcaggtgctg
      181 cacatgcagc cgaagtttat aataaagacg gcaacaaatt agatctgtat ggcaaagttg
      241 atggcctgca ttatttttct gataattcag cgaaagatgg cgaccagagc tatgcgcgtc
      301 tgggttttaa aggcgaaacc caaattaacg atcaactcac tggctacggt caatgggaat
      361 acaatattca ggcaaacaac actgaatctt caaaaaacca gtcatggacc cgtctggcat
      421 ttgccgggct gaaatttgca gattacggtt ctttcgatta cggacgtaat tatggcgtaa
      481 tgtacgacat cgaaggctgg accgatatgc tgcctgaatt tggcggtgac tcttatacca
      541 atgcagacac tttatgactg gtcgagccaa tggcgtcgcg acttatcgta atactgattt
      601 cttcggtctg gtaaatggtc tgaacttcgc ggtgcagtat caaggtaaca acgaaggagc
      661 cagtaatggt caggaaggca ccaacaacgg acgtgatgtt cgccatgaaa acggtgacgg
      721 ctggggtctt tccacaacat atgatttagg catgggcttt agcgctggtg cggcatacac
      781 ctcttctgac cgcaccaatg accaggttaa ccatactgcg gcgggtggtg ataaagcaga
      841 cgcgtggact gctgggctaa aatacgatgc taacaatatt tacctggcaa ccatgtattc
      901 agaaacgcgt aatatgaccc cgtttggcga cagcgattat gctgtggcaa acaaaaccca
      961 gaattttgaa gtcactgcac agtaccagtt tgattttggt ctgcgtccgg cagtctcttt
     1021 cctgatgtct aaaggccgtg acctgcacgc tgcgggtggt gcagacaacc cggcaggtgt
     1081 tgatgataaa gatctggtta aatacgccga tgttggcgcg acttactatt tcaataaaaa
     1141 catgtccacc tacgttgact ataaaatcaa cctgttggat gaagatgaca gcttcggtaa
     1201 tgactccaac ttattgatag tgttttatgt tcagataatg cccgatgact ttgtcatgca
     1261 gctccaccga ttttgagaac gacagcgact tccgtcccag ccgtgccagg tgctgcctca
     1321 gattcaggtt atgccgctca attcgctgcg tatatcgctt gctgattacg tgcagctttc
     1381 ccttcaggcg ggattcatac agcggccagc catccgtcat ccatatcacc acgtcaaagg
     1441 gtgacagcag gctcataaga
//
LOCUS       DAPHBJ010000359         1446 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.362, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000359 DAPHBJ010000000
VERSION     DAPHBJ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.362"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            119..1330
                     /gene="bluF"
                     /locus_tag="RHZ76_004549"
     CDS             119..1330
                     /gene="bluF"
                     /locus_tag="RHZ76_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HDV2815040.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
BASE COUNT          421 a          300 c          299 g          426 t
ORIGIN      
        1 actttttcca tttttatcaa tgtaaagaaa cggttatgtt ttggtattaa aagaaagaca
       61 caagaagata tgtctgttac cgtcttactc tcgcctcacc cattaccctg gattgttaat
      121 gcttaccacc cttatttatc gtagccatat acgtgacgac gaacctgtca aaaaaatcga
      181 agaaatggtt tcgatagcaa atcgcaggaa catgcagtct gacgtaacag ggatcttact
      241 gtttaatggt tctcattttt tccagcttct ggaaggtccg gaagaacagg ttaaaatgat
      301 atatcgggct atatgccagg atccacggca ctataatatt gttgagctgc tgtgcgatta
      361 cgcgcctgct cgccgttttg gcaaagcggg aatggaatta tttgatttgc gcctgcacga
      421 gcgagatgac gttttacagg ccgtattcga caaaggcaca tcaaaatttc agctaactta
      481 tgatgacaga gcgctacaat tttttcgtac ttttgtcctt gcaaccgaac aatcaaccta
      541 tttcgagatc cctgccgaag actcctggct ttttatcgct gacggatctg ataaagaact
      601 tgattcctgt gccctttcac caactataaa cgaccacttt gcctttcatc ctattgtcga
      661 tcccttatcg cggcggataa tcgcttttga agccattgtg caaaaaaatg aagatagccc
      721 atcagccata gcggttgggc agcgtaaaga cggggaaatc tacacagcgg atctcaaaag
      781 taaggcgctt gcattcacga tggcacacgc acttgagctc ggtgataaaa tgatttcaat
      841 caatctatta cctatgaccc tggttaacga acctgacgca gtctcttttt tacttaatga
      901 aataaaggcc aatgctctgg tgcctgaaca aatcatcgtt gaatttactg aaagtgaagt
      961 catatctcgg tttgatgagt ttgccgaagc gattaaatcg ctaaaggctg ctggtatcag
     1021 tgtagcaatt gatcattttg gcgcaggttt tgctggtttg ttactcctgt cacgcttcca
     1081 gcctgacaga attaaaatca gtcaggaatt gattaccaat gttcataaaa gtgggccacg
     1141 gcaggcaatt attcaggcga tcataaaatg ctgtacatca cttgaaattc aagtcagtgc
     1201 tatgggcgtg gcaacaccag aagagtggat gtggcttgaa tccgcaggaa ttgagatgtt
     1261 tcagggagat ctgtttgcta aagctaaatt gaatggtatc ccttccatag cgtggccaga
     1321 gaaaaaataa ttttcagcac attctttcac atgatttcag taaatcatcg tgaaaatgaa
     1381 tacgttacgt ttaaatttaa tccacattaa aaataatgtt tcatccaatt tagctgaaat
     1441 cttatt
//
LOCUS       DAPHBJ010000360         1442 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.363, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000360 DAPHBJ010000000
VERSION     DAPHBJ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.363"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            239..1114
                     /locus_tag="RHZ76_004550"
     CDS             239..1114
                     /locus_tag="RHZ76_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDV2815041.1"
                     /translation="MSDNNEVTHPFDVTNTETGKTYQLSPNSSKSVQPIALLRLSVFT
                     PVGTKEKRYRNFEVDASDELSSMELARSEGYDDIRITGLKLSMSTDFKCWLGCIMAFS
                     KYGFASDKITLSFNEFAKMCGISSTNINKRTRSRFQEALANLASVVISFRDSKTERFT
                     VTHLVQKAVIDPKKDTVELVGDPSMWELYRYDHKTLLSLQVLSVLAKKEAAQSLYIYF
                     EAMPAGTLFVSMKRLRERLLLTTPVRTQNQIIRKAMLELKSIGYLEYQEVKKGRDIQF
                     QIFKRSPKLALAKHS"
BASE COUNT          434 a          279 c          307 g          422 t
ORIGIN      
        1 tttagatcaa gagagatcaa gagagtacaa aaaagatcac agattacgta aatgactgat
       61 tttattatgg aataagcatg atcaggcatt caggtgcatg taaaaagcat ttgattgaat
      121 aagaaaagca tttacttgaa ttgaaaaggc atttagttta atgcgagaaa agcatttgca
      181 cgaataatct attcacatct aaatgtatca agggataaac catggtgggt aaatttaaat
      241 gagtgataac aatgaggtaa cacatccttt tgatgtgacc aacacggaga cagggaagac
      301 ttatcaactg agtccaaact cttcgaaatc ggtccaaccc attgctttac tgcgcttaag
      361 tgtttttacc ccggtgggaa ctaaagagaa gcgatacaga aattttgagg ttgacgcttc
      421 tgatgagttg tcgagcatgg agctggcccg ttcagaagga tacgatgaca ttcggatcac
      481 tggtcttaag ctttcgatgt caaccgattt taagtgttgg ctcggttgca taatggcgtt
      541 cagcaaatat ggtttcgctt ccgataaaat cacattgtca ttcaatgagt ttgcaaaaat
      601 gtgtggtatc agttctacaa acataaacaa gcgaactcgc tcacgatttc aggaggcgct
      661 agcaaaccta gcttccgttg tgatttcttt ccgtgattca aaaactgaac gttttactgt
      721 cacgcacctt gtgcagaaag cagtgattga tcctaagaag gacaccgtag agttggtggg
      781 cgatccctca atgtgggagc tttaccggta tgatcataaa accttactta gcttgcaggt
      841 gctttcggtt ctcgctaaaa aagaggcagc acaaagcctg tacatatatt ttgaggcgat
      901 gcctgccggt acgttattcg taagcatgaa gcgtttgcgt gagcgacttc tgttgacgac
      961 tcctgtacga actcaaaacc agatcatcag gaaggcgatg ctggagttaa aatcaatagg
     1021 atatcttgaa tatcaagagg ttaagaaagg tagagatatc cagttccaga tttttaaaag
     1081 aagtccaaaa cttgctctgg caaaacacag ttgatgctcc gggtatagta cgcacaaaag
     1141 ttactttgga tgtaatgagg agtatgggca ttcatccaaa tgctttttaa tgtttctccg
     1201 gatttgaaca cccttccctg tttccaaact tcctatcatt tctgataccc gggttcttct
     1261 ggtattcgtt cgaatgcttt tcagaaaaaa tcatgcttgc tagttgagac aacgtaacct
     1321 attcattttg ctagtttaat aagactcatt catacatatg cctttaacta cactaccctc
     1381 catcgtgttg tattgggctt taaacgcaaa tccctatact ccttgtgcca ctcggcgtat
     1441 tt
//
LOCUS       DAPHBJ010000361         1438 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.364, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000361 DAPHBJ010000000
VERSION     DAPHBJ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.364"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1143
                     /gene="nhaA"
                     /locus_tag="RHZ76_004551"
     CDS             <1..1143
                     /gene="nhaA"
                     /locus_tag="RHZ76_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005393226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HDV2815042.1"
                     /translation="VIRDFFKMESAGGILLVIAAAIAMTIANSPLGETYQSVLHTYVF
                     GMSVSHWINDGLMAVFFLLIGLEVKRELLEGALKSKETAIFPAIAAVGGILAPALIYV
                     AFNANDPEAISGWAIPAATDIAFALGIMALLGKRVPVSLKVFLLAVAIIDDLGVVVII
                     ALFYTGDLSTMALLVGFIMTGVLFMLNAKEVTKLTPYMIVGAILWFAVLKSGVHATLA
                     GVVIGFAIPLKGKQGEHSPLKHMEHALHPYVAFGILPLFAFANAGISLEGVSMSGLTS
                     MLPLGIALGLLVGKPLGIFTFSWAAVKMGVAKLPEGVNFKHIFAVSVLCGIGFTMSIF
                     ISSLAFGNVSPEFDTYARLGILMGSTTAALLGYALLHFSLPKKAQA"
     gene            1336..>1438
                     /locus_tag="RHZ76_004552"
     CDS             1336..>1438
                     /locus_tag="RHZ76_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012472779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDV2815043.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEM"
BASE COUNT          333 a          304 c          346 g          455 t
ORIGIN      
        1 gtcattcgtg actttttcaa aatggaatca gctggtggta ttcttctcgt gattgccgcg
       61 gcaattgcga tgaccattgc taactcaccg ctgggcgaaa cttatcaatc tgtactgcat
      121 acttatgtat ttggtatgtc agtttctcac tggattaatg acggcctaat ggctgttttc
      181 ttcttactca ttggccttga agttaagcga gagctgcttg aaggtgcgtt aaaatcaaaa
      241 gaaaccgcaa ttttccctgc gattgcagcg gttggcggta tattagctcc tgctcttatt
      301 tacgtagcct ttaatgccaa cgatcctgag gcgatttctg gttgggcaat tccagcggcg
      361 acagatatcg cttttgcttt aggtatcatg gcgttattag gtaagcgtgt accagtaagc
      421 ctaaaagtgt tcttgctggc ggttgctatc atcgatgatt taggtgtagt tgttattatc
      481 gcgctatttt acaccggtga cctgtcgacc atggcgttgc ttgttggctt catcatgact
      541 ggtgtcctat tcatgttgaa tgcgaaggaa gtaacaaaac ttacgccata catgatcgtc
      601 ggtgcgatct tatggttcgc ggtgcttaaa tctggtgttc acgcaacatt ggcaggtgta
      661 gtgattggtt ttgctatccc actaaaggga aagcagggcg aacattctcc acttaaacat
      721 atggagcacg cgcttcaccc atacgtggca tttggtattc tgcctctatt cgcatttgcc
      781 aatgcgggga tttcgttaga aggcgtatcg atgtcaggtc taacttcaat gctgccactc
      841 ggtattgctt tgggtctact ggttggtaag cctctaggta tctttacctt tagctgggca
      901 gcggtaaaaa tgggcgttgc taagttacct gaaggcgtta acttcaagca tatctttgcg
      961 gtatctgtgt tgtgtggtat tggctttaca atgtctatct tcatttcctc tttagcgttc
     1021 ggaaatgtaa gtcctgaatt tgatacctat gctcgactcg gtatcttaat gggttctacg
     1081 acagcagcat tacttggcta tgccttactg cacttttctt tgcctaagaa agcgcaagcg
     1141 taagataaaa atgagctatt ttgtaaagcc tcccaatcgg gataacaccg atcagttaag
     1201 gcattgatca gttgaacgat cctacggatg gttaacaata ccctcagtac actaattgct
     1261 gagggtattt tgggcactgt tgcaaatagt cggtggtgat aaacttatca tccccttttg
     1321 ctgatggagc tgcacatgaa cccattcaaa ggccggcatt ttcagcgtga catcattctg
     1381 tgggccgtac gctggtactg caaatacggc atcagttacc gtgagctgca ggagatgc
//
LOCUS       DAPHBJ010000362         1416 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.365, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000362 DAPHBJ010000000
VERSION     DAPHBJ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.365"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            209..1261
                     /locus_tag="RHZ76_004553"
     CDS             209..1261
                     /locus_tag="RHZ76_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV2815044.1"
                     /translation="MTNAATVLKSENSVQEEIIGNLLPANVISSKMFANKEQYYLVKA
                     KDLFGIPEMDEDMIVPVFKKFSPYRANLNKDYIPNSRILEQVVKLLVSNDIDLSVCLK
                     GESGSGKTEMAMYISHMLNWPLTIKQINSNIRADELEGERSLINGNTSFVPSELVTAF
                     QEGHLILLDEVDKIDPDTAAKLHMPIERKPWSLAANGGEVVKANKFTRFMGTSNTNMS
                     GGSRKFVSSQRQDAAFIKRFLIVEMERPSEVALTNVLLKRYNNLPMLVIEKFVSVAVA
                     VNNTGTDDSVMDIRQLVAWVGTSMTLKTMSLLDTFKIAFASALPAHATGMVLEAIDLI
                     LGDDKNRTMEYYTAKK"
BASE COUNT          425 a          268 c          335 g          388 t
ORIGIN      
        1 ttggttgggt aatcgtaaac cctacatttg aacgccagtt tgaatggatt gactcagaag
       61 tattcgaaat cgtaggaact catcttggtt acaacgttgc cgctatcaga acacagatgg
      121 cgacggcgtg taggatgact ttccactaac atgccactgg caaacatctg ccagcgggcg
      181 gtgtttgcat tttaccggag ttttaaaaat gactaacgcc gcaactgtat taaaatccga
      241 aaattccgta caggaagaaa tcattggtaa tctgttaccc gcaaatgtta tatcaagtaa
      301 gatgtttgcg aataaagaac agtattatct tgtcaaagca aaagatttat ttggcattcc
      361 tgagatggat gaggatatga ttgttccagt gtttaaaaag ttttctccat atcgtgcgaa
      421 tctgaataag gactatatac caaattctcg aatacttgag caagtagtaa aattgcttgt
      481 aagtaacgat atagatttga gtgtatgcct gaaaggagaa tctggatcag gtaagactga
      541 aatggcaatg tatattagcc atatgctgaa ctggccgcta accataaagc aaatcaacag
      601 caatattcgc gctgatgagc ttgaaggtga gcgtagtctt attaacggta acaccagttt
      661 tgtgcctagc gagcttgtaa cggcatttca ggaaggccac ctcattcttc tggatgaagt
      721 ggacaagatt gaccctgata ctgccgcaaa gcttcatatg cctatagagc gaaagccctg
      781 gtcactagct gccaacggtg gcgaagtggt taaggcaaat aagtttacgc ggtttatggg
      841 tacttcaaac acaaacatga gtgggggatc tcgtaagttc gtttcatcgc agcggcagga
      901 tgcagcgttt atcaagcgct tcctgattgt ggagatggaa aggccttcag aggttgcatt
      961 aaccaacgtt ttgcttaaac gctacaataa cctgcctatg ctggtaattg aaaaattcgt
     1021 ttcggtagcc gttgcagtaa acaacacagg aactgacgac agtgtaatgg atatacgcca
     1081 gcttgtggca tgggttggaa cgtcaatgac gttaaagacc atgtccttac tggatacatt
     1141 taaaattgct tttgcgtcgg ctctgcctgc acatgccact ggtatggtgt tggaagccat
     1201 cgacttaatt ctcggtgacg acaagaaccg tacaatggag tattacactg caaaaaagta
     1261 gttatagatg ggacgagtta tcagaaggct gaatgccgtg tgacagactt tgctcatctt
     1321 acgttttacg gttttctgag tgcaaacgga actgaaactt tcgtgattgc acaagatgga
     1381 aatcaatata taggtctgaa gcagcataat aatgac
//
LOCUS       DAPHBJ010000363         1410 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.366, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000363 DAPHBJ010000000
VERSION     DAPHBJ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.366"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            69..1298
                     /locus_tag="RHZ76_004554"
     CDS             69..1298
                     /locus_tag="RHZ76_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDV2815045.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTILQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPSLKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT          337 a          314 c          294 g          465 t
ORIGIN      
        1 catacttaac ctggtttatg gtaaattgcc ctccattttg tttaatttgt agatgatacg
       61 ttcagataat gtctaatatt tggtctaaag aagaaactct gtggagtttc gcgctctacg
      121 gcacagccgt tggtgcaggc acgctcttcc ttcctattca gttaggttcg gcaggggctg
      181 tggtcctgtt tattactgct ctggtcgcct ggcctttaac atattggcca cataaagcct
      241 tatgccagtt catcctctca tcgaaaacat cagcaggtga agggataacg ggcgcagtaa
      301 cacactacta tggcaagaag attggtaatc tgattaccac gctgtacttc atcgcctttt
      361 ttgtcgtcgt gttgatatat gcagtggcaa ttaccaactc acttacggaa cagctggcaa
      421 agcatatggt tattgatctt cgcatccgta tgttggtgag tctgggtgtt gtattaatac
      481 tgaatctcat ttttctgatg ggacgccatg caaccattcg ggtaatgggt tttttggtat
      541 tcccattgat tgcctatttc ttatttcttt ccatttacct tgtcggtagt tggcaacctg
      601 atctattaac gacccaggta gagttcaatc agaataccct tcaccagata tggatatcga
      661 ttcccgtgat ggttttcgcc tttagccata cgcccattat ttctacgttt gccatagaca
      721 gacgtgaaaa atatggcgaa cacgctatgg ataaatgcaa aaaaattatg aaagtcgctt
      781 atctcatcat ctgcataagt gtactgttct ttgtctttag ctgcctgctt tctattccac
      841 cttcgtatat tgaagcggct aaagaagaag gggtcaccat tttatcggcg ctttctatgc
      901 tgccgaacgc cccagcatgg ctgtcaattt ccgggattat tgtcgcagta gttgcgatgt
      961 cgaaatcatt cctgggtacg tactttggcg ttattgaagg tgccacggag gtcgtcaaaa
     1021 caatactaca gcaggttggt gtaaagaaaa gtcgtgcatt taaccgcgca ctatcaatta
     1081 tgttggtatc gctgatcacc ttcattgttt gttgcattaa cccgaacgcg atttcgatga
     1141 tttacgcgat cagcggcccg ctcattgcca tgatactttt catcatgcct acgttgtcaa
     1201 cgtatctgat cccgtcgctt aaaccctggc gttccatcgg aaatctgatt acgctgatcg
     1261 tgggtatcct gtgcgtatcg gtaatgttct ttagctgata tccattgtga atggttcaaa
     1321 attgctacaa ttttgaacca ttccagtttt cataatgttc attcgttatt attccgcact
     1381 atcacttccc tcatcatatc ctagtattat
//
LOCUS       DAPHBJ010000364         1403 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.367, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000364 DAPHBJ010000000
VERSION     DAPHBJ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.367"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..357
                     /locus_tag="RHZ76_004555"
     CDS             <1..357
                     /locus_tag="RHZ76_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV2815046.1"
                     /translation="KDEYYDAIESISPHITRHTWAYLTLQRIYRDKLQKIKANSHLAA
                     IDFSIVGLMDEAKDELRLLGGWSHNSHMPDLYAKRFLSQQANTANLQRIVIDNEALKS
                     TFSHVCDEWSAYESNQ"
     gene            341..907
                     /locus_tag="RHZ76_004556"
                     /pseudo
     CDS             341..907
                     /locus_tag="RHZ76_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078877.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(923..1403)
                     /locus_tag="RHZ76_004557"
                     /pseudo
     CDS             complement(923..1403)
                     /locus_tag="RHZ76_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          383 a          343 c          288 g          389 t
ORIGIN      
        1 aaagatgaat attatgatgc cattgaaagc atctcgcctc atataacgcg gcatacttgg
       61 gcttatttaa cacttcaacg tatttatcgt gataaacttc aaaaaattaa ggccaactca
      121 cacttggcag ccatagactt ttctatcgtt ggattgatgg atgaagccaa ggatgaatta
      181 cggctacttg gcggttggag tcacaatagc catatgcctg atctttacgc taaaagattt
      241 ctatctcagc aggctaatac ggccaatctt cagcgaattg tgattgataa cgaagccctg
      301 aaatcaacct tttcccatgt atgcgatgaa tggagtgcat atgagtcgaa tcaataaaca
      361 gttaaatgcg tcgaacagca acatttacac acctgtattt gttgagagta ttttaaaggt
      421 taatatacca gttagtattt atcttaaatc ggcagatcat tttgatgaca aatatatcag
      481 ctcaacaagt gacagttggg tattcgatta ttcaggaagc cgctgctatg cccactttcg
      541 tcaggataaa atcagcaacc aattaatcaa atatatttgc ttccattatg catctgccaa
      601 gtgtccagct caggttcctg gggtactcca tgcgtggtct ttggcaactg atcattgcat
      661 aagcaaatct gctttcactt ttacggtgtt gaaagattac ctcgaaactc aggaaattaa
      721 acctcaatat ttctacttta tcattttcgg cctaaaaatc ctctgtaccg aagcattccc
      781 tggttttacc ctcgaagatt atgaagacct cgaattcatc cctcgcccgc actcccatga
      841 ctggggaatc tatcaggaaa ttgatcatgt tctggatccc ctcgaaaaaa acatgatcag
      901 taaaggcggt aatgactcca acttattgat agtgttttat gttcagataa tgcccgatga
      961 ctttgtcatg cagctccacc gattttgaga acgacagcga cttccgtccc agccgtgcca
     1021 ggtgctgcct cagattcagg ttatgccact caattcgctg cgtatatcgc ttgctgatta
     1081 cgtgcagctt tcccttcagg cgggattcat acagcggcca gccatccgtc atccatatca
     1141 ccacgtcaaa gggtgacagc aggctcataa gacgccccag cgtcgccata gtgcgttcac
     1201 cgaatacgtg cgcaacaacc gtcttccgga gcctgtcata cgcgtaaaac agccagcgct
     1261 ggcgcgattt agccccgacg tatccccact gttcgtccat ttccgcgcag acgatgacgt
     1321 cactgcccgg ctgtatgcgc gaggttaccg actgcggcct gagtttttta agtgacgtaa
     1381 aatcgtgttg aggccaacgc cca
//
LOCUS       DAPHBJ010000365         1403 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.368, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000365 DAPHBJ010000000
VERSION     DAPHBJ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.368"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..803
                     /locus_tag="RHZ76_004558"
     CDS             <1..803
                     /locus_tag="RHZ76_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005183576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDV2815047.1"
                     /translation="VRDLIAANDSGVLPEFSLNDAFKFVTDVAAKDIMFMLKSAEINT
                     ALSDEGQRKNYGLNVSGALSQARKNGFISTDLLSQMLINTTAASDARMGGAPLPAMSN
                     YGSGNQGITVTMPVVTLARHLNTDDETLARALALAHLAAISIHMRYTRLSALCAASTA
                     AMGAAAGMSWLLTQDFQTISYAVSNMISDISGIICDGASNSCAMKVSTATACAFKSVL
                     MAQQNSVAGERDGIVSCDVEGSINNLCKLVLSPMRQTDKEIISIMTRK"
     gene            885..>1403
                     /locus_tag="RHZ76_004559"
     CDS             885..>1403
                     /locus_tag="RHZ76_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004711301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HDV2815048.1"
                     /translation="MSKSKSIATLVIHAGDQVDRGNNAIFPPITTASSFIQPNLGEEG
                     EFCYSRCSNPTRYAYETALAELEGGIYATATASGMAATALILELLPKDSHVLVMKGVY
                     GGTYRLFERLRKQTSGTTFSYVDLNDLASVERHIQENTGLIWIETPTNPLLELVDIEA
                     VCQVAKSRGIMSC"
BASE COUNT          391 a          290 c          343 g          379 t
ORIGIN      
        1 tagtccgaga cctaattgcc gccaatgata gtggggtact acccgaattt tcccttaacg
       61 acgcatttaa atttgtaacc gatgtcgccg ctaaagacat catgtttatg ctgaaaagtg
      121 ctgagattaa taccgcgctt tctgacgagg ggcaaaggaa gaactatggt ttgaacgtga
      181 gtggcgcgtt gtcgcaagcc agaaaaaatg gctttatcag cactgactta ctgagtcaaa
      241 tgttgatcaa caccactgcg gcatcagatg cccgtatggg cggcgcaccc ttacccgcga
      301 tgtcaaatta tggctctggt aatcagggga ttaccgtgac aatgccggta gtgacacttg
      361 cccgacatct taatacagat gatgaaacac tggcccgggc attagcgttg gcccatctag
      421 ccgcaatctc aattcatatg cgttataccc gattgtctgc attgtgtgcg gcgtcaacgg
      481 cagcaatggg ggcggcggca ggtatgagtt ggttattgac ccaagatttt caaactatct
      541 cctacgcggt cagtaatatg atcagtgata ttagtggcat tatttgcgat ggtgcatcaa
      601 atagttgtgc catgaaagtg tctaccgcta ccgcctgtgc atttaagtcg gtgttgatgg
      661 cgcaacaaaa tagtgttgca ggggagcgtg atggcattgt ttcctgcgat gttgaaggtt
      721 ctatcaataa tttatgtaaa ttagtgttat cgccaatgag gcaaacagat aaagaaatta
      781 tcagtataat gacacgtaaa taaatattac ggtttaaccg cgtggcctaa cgtcattatt
      841 aataatgatc ggcgggctat cgttaaaatt taaagggaca aataatgagc aaatcaaaat
      901 cgatcgcgac tctggttatc catgccggag atcaagttga ccggggcaat aatgctattt
      961 ttccaccgat tactaccgcc agttccttca ttcagccaaa tttaggcgaa gaaggggagt
     1021 tttgttattc ccgttgcagt aacccgacaa gatatgcata tgaaaccgcc ttggctgagt
     1081 tggaaggagg tatctatgct acggcaaccg cttccggtat ggcagcaact gcacttatat
     1141 tggaattgct acctaaggat tcccatgtat tagtaatgaa aggggtctat ggcggtactt
     1201 atcggttgtt tgaacgtctg cgtaaacaaa cttcaggtac gacattcagt tatgtcgatc
     1261 ttaatgatct ggcgtcagta gagcgccata ttcaagagaa tacaggtcta atctggatcg
     1321 aaacgcccac taacccactg ttggaattag ttgatattga agcagtatgc caggttgcca
     1381 aaagcagggg cataatgagc tgc
//
LOCUS       DAPHBJ010000366         1373 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.369, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000366 DAPHBJ010000000
VERSION     DAPHBJ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.369"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            46..852
                     /locus_tag="RHZ76_004560"
     CDS             46..852
                     /locus_tag="RHZ76_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815049.1"
                     /translation="MANNYTQASFTILCSQEQAQMALDAIAYVTDTDVAEGEHLLSKP
                     VSECSLTELLVLGIIQNHPECDPFEPTFGQSESPEDNYELELKAEITGKGLAICHDES
                     INLDHAIAVTTAVLSVFNLPEMVTINAAFVCDRPRENEFGGATIVVTKDTHHYEEGFN
                     FSRLMNEAHDAGVQYALVKVNQYNHEYTYTQCYLMSCKKSESAYDVARHRLASDESTP
                     DNPGEDGVIILSEEDNTSMALHSVTELMPVVYESLSKLLPSLDELCPVTA"
     gene            900..1346
                     /locus_tag="RHZ76_004561"
     CDS             900..1346
                     /locus_tag="RHZ76_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815050.1"
                     /translation="MTNKILILSASCINEFASDVPQSVFFSIDTSLAKRIRELAAYVK
                     ENDLYAAEFFFYDCHWSETLEEDIEVLMSQAAFIESDSSGQETMLRDVMPSCRTETTS
                     IRVMKESFHLTAVPRHCGDDMMLNTPLIPLAELESNATVLITQQYS"
BASE COUNT          370 a          336 c          334 g          333 t
ORIGIN      
        1 tattcacaga cagcaatcac atttaattaa ataaggagtt atgcaatggc gaacaattac
       61 actcaggcat ccttcacaat tctctgttca caggaacagg cacagatggc actggacgcg
      121 attgcgtatg ttacagacac ggatgtggcg gaaggtgaac acttgctgtc taaaccggtt
      181 tcagagtgtt ccctcactga attgcttgtc ctgggtatta ttcagaacca cccggagtgc
      241 gatccgtttg agccgacttt tggtcagtcg gagtcaccag aagacaatta cgagctggaa
      301 ctcaaagcgg aaatcaccgg taaaggcctg gcaatctgcc atgatgaaag cattaacctc
      361 gaccacgcta tcgcggtaac aactgccgtg ttgtcggtgt ttaacctgcc tgagatggtc
      421 acaatcaatg ccgctttcgt ctgcgaccga ccacgcgaaa acgagttcgg cggtgcgact
      481 attgtggtga ctaaagatac acaccactac gaagaaggct ttaatttttc tcgcctgatg
      541 aacgaagctc atgatgccgg tgttcagtat gcgttggtga aagtgaacca gtacaaccac
      601 gaatacacat acactcaatg ctatctgatg agctgcaaaa aatcagaatc ggcttatgac
      661 gttgcacgcc accgcctggc ctctgacgag agcacgcccg acaatcctgg tgaggatgga
      721 gttatcattc tcagcgaaga agacaatacc agcatggcgc ttcacagcgt cactgaattg
      781 atgcccgtcg tgtacgaaag cctgagcaaa ttactgcctt cacttgacga attgtgcccc
      841 gtgactgcct gataatccct gattgccacc ggaacagccg gtggcatgga gagctgaaaa
      901 tgaccaataa aatcctgatt ttgtccgcta gctgcatcaa tgaatttgcg agtgatgtcc
      961 cgcaaagcgt cttcttttcc attgatacgt cgctggctaa gcgtatccgt gaactggcgg
     1021 catacgtgaa agaaaatgat ttgtacgccg ccgagttttt cttctatgac tgccactggt
     1081 ccgaaacatt ggaagaggac attgaggttc tcatgtcgca ggctgcgttt attgaaagtg
     1141 acagcagcgg acaggaaacg atgctccggg acgtaatgcc ctcctgccgc acggaaacaa
     1201 cttccatccg ggtcatgaaa gaatcatttc atctgacggc tgtaccccgt cattgcggcg
     1261 atgacatgat gctcaatacg cccctaattc cgttggcaga gctggaatca aatgcgacgg
     1321 ttttaattac ccagcagtat tcgtgagagc gtggagaaca taatatattt att
//
LOCUS       DAPHBJ010000367         1339 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.370, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000367 DAPHBJ010000000
VERSION     DAPHBJ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.370"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(10..>1339)
                     /locus_tag="RHZ76_004562"
     CDS             complement(10..>1339)
                     /locus_tag="RHZ76_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDV2815051.1"
                     /translation="TVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYLMVMIALFFID
                     TVAFIFMQLYFIYDRRQFSNCVLSLAFLSCLIYFVKTVIIIQQIIEERLTSSVVQNDI
                     AIYYLFRQMSLYILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFGGPIVAHILSS
                     HYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRYDIWNGVTVIA
                     FCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALRVTKNIAHRDP
                     LTNIFNRNYFFNELTVQSASAQKTPYCVMIMDIDHFKKVNDTWGHPVGDQVIKTVVNI
                     IGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGDNPEYAIPQKV
                     TISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCESP"
BASE COUNT          445 a          301 c          219 g          374 t
ORIGIN      
        1 aatgccgctt tatggtgact cacaaaaatt caccacatcc ctcacaacca ccttattacg
       61 cccggtctct ttcgcctcgt aaagtgcgtt atcagccagt cgatagatct catttgggtt
      121 taacgcattt tcctgagtca caacagcgcc aatactaatc gtcacttttt gtggtatagc
      181 gtattcagga ttatcgccag ttaaacgctc aacattttcc cgaatccttt ccgctaaagc
      241 tttcgcacgt tcagtatcga tgtccgtcag caagacgcca aactcttcgc cgccgacgcg
      301 cgctaaaagg tcatctggtc gtatgctttt cccaatgata ttcaccactg tttttatcac
      361 ctgatcgcca accggatgcc cccaggtgtc gttgactttt ttgaagtggt cgatatccat
      421 aatcatgacg caataaggcg ttttttgggc tgatgctgat tgaactgtca gttcattaaa
      481 aaaataattt ctgttaaata tattggttaa gggatcgcga tgtgcaatgt tctttgttac
      541 tcgtagcgca ctgaatatat ggcacataaa tattaccata acggttaact tactgactac
      601 ttcaatagtg cgactaatat accaagtgga cacgctatac ctgctcataa ataacagaga
      661 gatattgtac agtacggcac aaaaagcaat caccgtcact ccattccaga tatcatagcg
      721 taaaccatta aagtatagat taaccgaaag caaggttaac cacatgaaaa tcattatggt
      781 aacatatgag gctttccaaa ccacctgacc attttcattg gttaactcgg caatgtgtaa
      841 attgtagctc tcataatgac tagaaagtat gtgagcaact atcggccccc caaaaacaaa
      901 aaacaaactt atgcacaaag tcattttttt ggaaaataaa ttgcgctgtt ttgtgttttc
      961 actaacttta ttcaccaatg ccagaaatat taatatgtac aaactcatct gacgaaacaa
     1021 ataatagatt gcaatgtcat tttggacaac actgcttgtc aaacgctcct caataatttg
     1081 ctgaatgata atgacggttt taacaaagta aatcaaacaa ctcagaaaag ccaaactaag
     1141 tacacagttt gaaaattgcc tacggtcata aatgaaatac aactgcataa aaataaatgc
     1201 aacagtatcg ataaaaaata aagcaatcat taccatcaga taggttgatc gtggaacaac
     1261 ttcctcaacg cctggctgat taaaccaact attcaccaaa accaaagcaa gcccaaacaa
     1321 taagctcact aatacagta
//
LOCUS       DAPHBJ010000368         1338 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.371, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000368 DAPHBJ010000000
VERSION     DAPHBJ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.371"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            71..475
                     /gene="stpA"
                     /locus_tag="RHZ76_004563"
     CDS             71..475
                     /gene="stpA"
                     /locus_tag="RHZ76_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HDV2815052.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLL"
     gene            complement(522..1046)
                     /gene="ygaP"
                     /locus_tag="RHZ76_004564"
     CDS             complement(522..1046)
                     /gene="ygaP"
                     /locus_tag="RHZ76_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HDV2815053.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRHEQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1056..>1338)
                     /locus_tag="RHZ76_004565"
     CDS             complement(1056..>1338)
                     /locus_tag="RHZ76_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2815054.1"
                     /translation="LQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGELTRITGL
                     SASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          356 a          324 c          318 g          340 t
ORIGIN      
        1 ataataagtg atcgcttaca ctacgcgacg aaatactttt ttgttttggc gttaaaaggt
       61 tttctttatt atgtccgtaa tgttacaaag tttaaataac attcgcactc tccgtgcgat
      121 ggctcgcgaa ttctccattg acgttcttga agaaatgctc gaaaaattca gggttgtcac
      181 taaagaaaga cgtgaagaag aagaacagca gcagcgtgaa ctggctgagc gtcaggaaaa
      241 aattagcacc tggctggagc tgatgaaggc tgacggaatt aacccggaag agttattagg
      301 taatagctct gctgctgcac cacgcgctgg taaaaaacgc cagccgcgtc cggcgaaata
      361 taaattcacc gatgttaacg gtgaaactaa aacctggacc ggtcagggcc gtacaccgaa
      421 gccaattgct caggcgctgg cagaaggtaa atctctcgac gatttcctgc tctaattact
      481 gaggccggat gtcgctgcta gggcgcgtcc ggcgtcgctt ctcaagctcg ttggttccac
      541 ggcatcttat ctaacaacct tgccattcca caaaaaccgc tgattcctgc aaacagtaac
      601 ccggcaccaa caaagccact taataagaag aaaccgctat ttacggtata acccagtaca
      661 acgccgatta atattaaacc acccgcagcg atctgcacct ggcgcattaa cggcaagggt
      721 tgagatttat ttaccgctac tggcaatcca gctcttttcc agccatcaat cccatcttcg
      781 agtaaaaata tttctgcggg ggcggcaatc gctgctaatt tatcggcatt attactggtg
      841 cgtttacctg cctggcagtg gaatataatt tgctcgtgac gtaatttagc cggaagacct
      901 gactgttcca gcacggataa tggagccaga tctgcttcag gaatatgttc acgaagatat
      961 tcatcagcat cacgaatatc gattaacttt gcgccgcgtg cgattaattc ttgtgcatca
     1021 tgcggcgaaa tggttgtcaa agccatagtg actccttacg gacaatagac atttttcagg
     1081 gtggcgataa tggcatttac cgcctcattt ttaatggaat atagaatgcg ttgggcatcc
     1141 cgttggctgt cgataagccc ttcgtcccgc atacgagcga gatgctgtga tgtcgcagag
     1201 gcactcagtc cggtaatgcg cgtcagctct cccgcgctgg tgccggggga accgctaagc
     1261 atgcacagaa tcagcaatcg tttaggatgg ctcattgctt tcaataaggc cgctgcctgt
     1321 tcggcactgg cctgtaat
//
LOCUS       DAPHBJ010000369         1329 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.372, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000369 DAPHBJ010000000
VERSION     DAPHBJ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.372"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..338)
                     /locus_tag="RHZ76_004566"
     CDS             complement(<1..338)
                     /locus_tag="RHZ76_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HDV2815055.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKR"
     gene            complement(559..1290)
                     /gene="bluR"
                     /locus_tag="RHZ76_004567"
     CDS             complement(559..1290)
                     /gene="bluR"
                     /locus_tag="RHZ76_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HDV2815056.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSIFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          338 a          308 c          300 g          383 t
ORIGIN      
        1 ctgcgtttgt tatgattaac atcagtaacc caccggaaaa cgcccgcgcc tgccagtgtt
       61 gaacagtatt cccgaaatgt agattttccg caaatatgaa gcaatgcggc ctcttttatt
      121 ttagcagggt tcttggtcgt actaactttt aacaggttcc tggttcctct taataacaaa
      181 accgtatcat cgtgagtaat aattctgatg ttatccgtag ccagataata aatgtaatgt
      241 gcaatacggt gatgttttaa ttctgaataa aaccaggaga agttttgctc ttttctcact
      301 tgctcaaaca tcttttgaaa aacaacgacc tgatccatca ggataataac ctcttgttag
      361 ttgtgagact gcgtagtgtg cacgatcggt tttaccactt caatctggtc tgtcctttgg
      421 ctgtgatatg tacagagtgt gatagaggga atatctgaat tctcccggtg agcattttgc
      481 aacggaccag ctccggtaca aacgctgttg tgggttcaga ttataacatt ctgtctaagg
      541 ggcgggataa aggtgaaatt agggggcatg aaagatgact ttataacctt gctcacccca
      601 gtgttgtaaa agttcgtttt gccttctcgt tggtgccatg cctgtccaga caatcaatgt
      661 ttgcgtcggg aacagttccg ggcgcggcga ttcaatggga tcagcaagaa cagaaatgtg
      721 ccatcctgac aatgataaac gccatgcttc cagccacaat cgtgctctct cttcaacatt
      781 ccacgccatc agaatggctt ctttaccggg cttacggcgc atttcgaaaa gcgaggttgc
      841 tgcgtattca attaatgcgc cgtcaaatat actgctcata atgcgggagg tgttgtgatc
      901 aagcacgaga cgctggcgaa caggaaggta aacatgatta atcaattgat ctactgggta
      961 ctctcgaccc agtgaaataa ttctcgcgcg tagtttggca ggattagcca tgcgaagaat
     1021 tgacatcatc tcttcttgca ggcggctcca gtcatcttcc gtatcctggc tggtggtttc
     1081 cagtaatgct ttaactttgc ctacagggac gccattactt atccaacgct tgatctcttc
     1141 gatgcgttgt atgtcttctt catcaaagag tcggtgtccg ccttcactgc gctgtggttt
     1201 taacaaaccg tagcggcgtt gccaggcccg gagagtgaca ggattaatcc cgcaacgttc
     1261 agcaacatca ccaatgctgt aataagccac aattcctcct tgcggtcaca aatctccgtc
     1321 gcctgtaca
//
LOCUS       DAPHBJ010000370         1319 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.373, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000370 DAPHBJ010000000
VERSION     DAPHBJ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.373"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            183..785
                     /locus_tag="RHZ76_004568"
     CDS             183..785
                     /locus_tag="RHZ76_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDV2815057.1"
                     /translation="MLAFCRSSLKSKKYIIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDSLVMHVEKGKVYLDGEFVNH"
     gene            complement(824..1249)
                     /gene="nudI"
                     /locus_tag="RHZ76_004569"
     CDS             complement(824..1249)
                     /gene="nudI"
                     /locus_tag="RHZ76_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HDV2815058.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWAISGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
BASE COUNT          366 a          289 c          274 g          390 t
ORIGIN      
        1 tagacgtaaa tattgaaatt tttatatttt ttcttattta ggctttgcat ttggcaaaat
       61 tttgaggcat tttgccgaca tcgtaggatt tttaatatta caccaactgc gaattatcgc
      121 cagaaatgtc gctcaatttc acggtaattg tctggttgcg cttgtctata ggtggagttt
      181 acgtgttagc tttttgccgc tcttcgttga agtcaaaaaa atatatcatc attttactgg
      241 cgctcgctgc aattgccgga ctgggtactc atgccgcctg gagtagcaat ggtttgccac
      301 gtatcgacaa taaaacactg gccagactgg cacagcagca cccggttgtc gttttgtttc
      361 gtcatgctga acgttgcgac cgttcaacca atcaatgctt gtcagataaa acaggtatta
      421 cggttaaagg tacccaggat gcccgtgaac tgggcaacgc ttttagtgct gatatccctg
      481 atttcgatct ttattccagt aataccgtcc ggaccattca gtcggctacc tggttttcag
      541 cgggtaaaaa attgacggta gataaacgac ttcttcagtg cggtaatgag atttatagtg
      601 caattaagga cttacaaagc aaagcgcctg ataaaaatat cgttattttc acccataatc
      661 attgcctgac atatattgct aaagataagc gtgacgcgac atttaaacct gattatctgg
      721 atagtttagt catgcatgtg gaaaaaggca aagtttatct ggatggggaa ttcgttaacc
      781 actaaatttt caatctgaca gccagtaatg gctgtcatca ttgttacaga agacctttca
      841 aacgtaacgt ttttcgggtg gcgacattca aatcataatg caccagatct tcaggtttta
      901 cccacgcgta gtcctgaaac tcttcgttta ttttcacttc tcggttggca gaaacgcagt
      961 caaaaatcag gtaaatcata taaatctctt ccttgcgacc atctgcatac gtcttggtgc
     1021 gaatatcatc gctgaaggtc cacggcgtga tttctgtcaa aagcagctgt tctcccagtt
     1081 cttcgcgaat ttcgcggcgt agtgcctctt caattcgttc gccaggctcc acgccgccac
     1141 ccgaaatcgc ccattgaccg gggaaaacgc cgcgatcgtc ggccatttta cacagcaaat
     1201 aagcaccatc attttgaatc aaagggcata caatagtccg ttgtcgcaca gggatattcc
     1261 ttataaataa aaagaaacca tgagaatacg gtaactcaca aatatgtgaa agtgacagt
//
LOCUS       DAPHBJ010000371         1318 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.374, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000371 DAPHBJ010000000
VERSION     DAPHBJ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.374"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(251..>1318)
                     /locus_tag="RHZ76_004570"
     CDS             complement(251..>1318)
                     /locus_tag="RHZ76_004570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815059.1"
                     /translation="DIIEVSRLATFAPSLQEISIKEMMRVWNLYSTQSISSLFKTLNV
                     APETFQSFIDKYGIPADEQIQYPWQDSRIQQLFDDSGFKETLWQHVMQQRALLKNYFA
                     TKGLTDDINARICVVDVGWRGTIHDNIALLYPDIHFTGIYLGLQKFLNEQPSNTSKVA
                     FGPDLNHQLEYPHFLDSVAPIEMITNSPSGSVTGYGLENGKIVAIRSVNDDENSAWHN
                     FTKTFQEGILAGMESFSAAVLSYGITHDVVRGYALNIWDVLISGSNKSLTDAFNNLNH
                     NETFGLGGYVKKNHVPTTFEILSSLWNKNNRAALIEFIKANQWSDGIRKRDNLPSLNK
                     YILALTIDLAVFYKRKFYRKY"
BASE COUNT          395 a          274 c          276 g          373 t
ORIGIN      
        1 ccgtcagtag ctgaacagga gggacagctg atagaaacag aagccactgg agcacctcaa
       61 aaacaccatc atacactaaa tcagtaagtt ggcagcatca ccggctgatt cgatgtattt
      121 gcgggaaaaa aatcggccca gatccgcgaa attttaatca gcgagtcagc tggggaagaa
      181 atgacctgct tattcgcacc ttccctaact ttaaaaaaaa gcatctgtcg atgccctgaa
      241 taaaaagtaa ttaatacttg cgatagaatt ttcttttata aaaaaccgca aggtcaatcg
      301 tcagtgctaa aatgtattta tttagcgagg gcagattgtc tcttttacgt atgccatcgc
      361 tccattgatt tgctttaata aattcaatca atgctgcacg gttgttttta ttccataaag
      421 agcttaatat ctcgaaggtc gtgggtacat ggtttttctt aacgtatcca cctaagccga
      481 atgtttcgtt atgatttaaa ttattaaaag cgtcagttag agatttatta ctaccactaa
      541 ttagcacatc ccaaatattt agggcgtagc cacgtacaac gtcgtgagta atgccataac
      601 tcaacactgc agcggagaag gattccatac cggccaggat cccttcttga aatgtttttg
      661 tgaaattatg ccacgcgctg ttttcgtcgt cgttaacact gcgaatagcg actatcttac
      721 cattctccag cccatagcct gttacgctac ctgaagggga gttggtaatc atttcaatag
      781 gagcaacgga atcaagaaaa tgaggatact ccagttggtg attcaaatct gggccaaaag
      841 caacttttga tgtgtttgat ggttgctcgt tgagaaattt ctgcaggccc agatatatgc
      901 cggtaaaatg aatatcagga taaagaagag caatattatc gtgaatggtg ccacgccagc
      961 cgacatcgac tacgcaaata cgcgcattga tatcatcagt tagacctttt gtcgcaaaat
     1021 agttcttcaa aagagctctt tgctgcatca catgctgcca gagcgtctct ttgaaaccag
     1081 agtcgtcaaa aagttgctga atacggctgt cctgccatgg atattggatc tgttcatctg
     1141 caggaatccc atatttgtcg atgaaactct ggaatgtttc aggggcaacg ttaagggtct
     1201 taaataaaga gctaatggac tgtgtgctat ataaattcca aacacgcatc atctctttta
     1261 tgctaatttc ttgtagagac ggcgcgaagg tagccagacg gctgacttct ataatatc
//
LOCUS       DAPHBJ010000372         1306 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.375, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000372 DAPHBJ010000000
VERSION     DAPHBJ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.375"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..473)
                     /locus_tag="RHZ76_004571"
     CDS             complement(<1..473)
                     /locus_tag="RHZ76_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDV2815060.1"
                     /translation="MNRTSPYYCRRSVLSLLISALIYAPPGMAAFTTNVIGVVNDETV
                     DGNQKVDERGTTNNTHIINHGQQNVHGGVSNGSLIESGGYQDIGSHNNFVGQANNTTI
                     NGGRQSIHDGGISTGTTIESGNQDVYKGGISNGTTIKGGASRVEGGSANGILIDGG"
     gene            complement(538..657)
                     /locus_tag="RHZ76_004572"
     CDS             complement(538..657)
                     /locus_tag="RHZ76_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDV2815061.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(812..1030)
                     /locus_tag="RHZ76_004573"
     CDS             complement(812..1030)
                     /locus_tag="RHZ76_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV2815062.1"
                     /translation="MKKLVRTSSAGNALLVCKLNRLGRSMWHLVVLLEELCERGINFR
                     ALAQSIFAQQWGDECCKSKTICDLKVIV"
BASE COUNT          368 a          313 c          262 g          363 t
ORIGIN      
        1 ccaccatcaa tgagtattcc attcgcactc cctccctcta cgcgtgaagc accgccctta
       61 attgtcgttc cattgctgat acccccttta taaacgtcct gattgccact ctcgattgtc
      121 gtacctgtgg aaataccccc gtcatgaatt gactgtctgc caccgttaat ggttgtatta
      181 ttagcctgcc ccacaaaatt gttatgactt cctatatctt gatatccacc agattcaata
      241 agacttccat tagatacccc gccatgaaca ttctgctggc catggttgat aatatgagtg
      301 ttatttgttg tacctcgttc atccactttt tggttgccat ctacagtctc atcgtttacc
      361 acaccaataa cattagtagt gaaggcagcc atcccgggcg gggcatatat caaggcagat
      421 atcaataagg aaagtactga gcggcgacaa taatagggac tggtcctgtt cataaatttc
      481 atcctctgaa aagtgaatac tgagtagcgt ttaagcgacc ttagctttgc tgcaacatca
      541 gcccacaggc accagaccag gggattcatc ctgaagagac agcgcaagtg tattgtgttc
      601 accgctcatc aaagacatca tgatgaaatg atgatattcc gcataagaat gaggcatttt
      661 ttaaacgcag tgcgctgaag tgtggttgga taaaaaagtc aatccattca ggaaatacgg
      721 gcgtattctt ttctttcgac aatgaggccg ttggcaaaat aaaatgattt acataatcgt
      781 ttctgatgaa tatcttctgc tcacataaaa atcacacaat aactttgaga tcgcagattg
      841 ttttactttt acagcattcg tccccccatt gttgggcaaa tatagattgg gccagagcac
      901 gaaagttaat accacgttcg cacagttcct ccaacagcac gacaagatgc cacatactgc
      961 gccccagtcg gttcagttta cagactagca gtgcgttccc tgccgatgat gtcctgacca
     1021 gttttttcag tccggacctc ctgccacggt tttacgttga agtggtaacc ctgagcacgc
     1081 agttcttcag tcaggcgtgg tgcaccgtaa cgctgttatt gctgggtaag atcaaaaaac
     1141 tttcaggcag ctaaggaaag ttgaaccaga cattagatga aatatttcaa ccaattacag
     1201 caccaattca gtcactgcca gcccaccaaa taaatcaagg ggttacatga aaacgtagcc
     1261 cctttttctt tggtagtgac actaaaatgg atgtagtgtg aagaat
//
LOCUS       DAPHBJ010000373         1294 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.376, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000373 DAPHBJ010000000
VERSION     DAPHBJ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.376"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..331
                     /locus_tag="RHZ76_004574"
     CDS             <1..331
                     /locus_tag="RHZ76_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDV2815063.1"
                     /translation="NGDVRNWAAFAININKYDNSDIRDCFVEMLQDKNEEVRIEAIIG
                     LSYRKDKRVLSVLCDELKKNTVYDDIIEAAGELGDKTLLPVLDTMLYKFDDNEIITSA
                     IDKLKRS"
     gene            359..828
                     /locus_tag="RHZ76_004575"
                     /pseudo
     CDS             359..828
                     /locus_tag="RHZ76_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          385 a          253 c          329 g          327 t
ORIGIN      
        1 aaatggagat gtcagaaact gggccgcatt tgcaataaat atcaataaat atgataatag
       61 tgatattagg gattgttttg tggagatgct tcaggataaa aatgaggaag tccgtattga
      121 agcaataatc ggactttcct acagaaaaga taaaagggtt ttatctgttt tatgcgatga
      181 gttaaaaaaa aatactgttt atgatgatat cattgaagct gcgggtgaat taggtgataa
      241 aacgctactt cctgttttag atactatgtt gtacaagttt gatgacaatg aaattataac
      301 ttccgctatt gataagctga agcgttcata gcgtgagttg cctatgcaca gtgggggatt
      361 cccgccggca cggtgcagat gctcgaccgg ctggaaagtg aaatcctggc tgaccgggtg
      421 agtgaggaaa gccgccgctg gctggcatcg tgcggcctga ccgtggagca gatgaaaaac
      481 cagatggacc cggtgtacac gccggcgcga aaaatccacc tgtaccactg cgaccatcgc
      541 ggcctgccgc tggcgctcat cagcacggaa ggggcaacag cgtggtgcgc agaatatgat
      601 gaatggggca acctgctgag tgatgagaac ccgcatcatc tgcagcagct cattcgcctg
      661 ccgggtcagc agtatgatga ggagtccggc ctgtattaca accgccaccg ctattatgac
      721 ccgctgctgg ggcggtatat cactcaggat ccgattggac tgaagggggg atggaatttt
      781 tatcagtatc cgttgaatcc ggtcataaat gtagatccgc aaggtttggt tgatataaat
      841 ttataccccg aaagtgatct tatccattct gtagctgatg agattaatat cccaggcgtt
      901 ttcacaatcg gggggcatgg tacccccaca tctattgaat ccgcaacgcg cagtatcatg
      961 acagctaaag atctagcata tctaattaaa tttgatggga attataaaga tgggatgaca
     1021 gtttggttat tttcttgtaa tacaggtaaa ggacaaaatt catttgctag ccaattagct
     1081 aaagagttac atacaaatgt aataggacct gacacgctat ggacgtggtg ggggcgagga
     1141 actaatggta agttaaaaat ggatacagtg ctaacagcac caacgaacct taattcaaat
     1201 aaggatctaa tggctataac aacaaaagac cttggtaatt ggataacata tgggccatct
     1261 gggcacccca tttctaatat gcaaggtacg ccag
//
LOCUS       DAPHBJ010000374         1282 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.377, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000374 DAPHBJ010000000
VERSION     DAPHBJ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.377"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(457..1089)
                     /locus_tag="RHZ76_004576"
     CDS             complement(457..1089)
                     /locus_tag="RHZ76_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HDV2815064.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          345 a          272 c          259 g          406 t
ORIGIN      
        1 atatcttcta taaataaccc gcaaaatatc gtcgtagctg cactcgatat ttttaaaatc
       61 ctgtatcaag tgctgaaaat catttcatcg ccacttccaa ttcctttctg catggtaagt
      121 agtgaggacg aaaagaagaa tatgataaat attgattcgt ggtaatcaga gagagtcgcg
      181 acatttgtga tgttattctt acaactgtaa gaagcgccgg tgatttacaa gcaagggtat
      241 gattacgctt tttaaagcgt accataaaac ataacataat aaatgatttg tgatgacacg
      301 ggattattgc ggtttacttt gaggaatata aaatgcgagt ttatagcacc gcgagcgcca
      361 aataatttta tgaccacaac ctgtcgtatt gtggtcataa aaaattgtcg gctggaggga
      421 gttaaatcct ttacctcaat atactgcctg tcagcattag ttcacaaaca gtgcttcgct
      481 atcgacgatg attccatctt ccgcaccttt aagcgccagg tttgcctcgt agtattgctt
      541 tttatcaagc agtttctgcg catcagcaag tgcattacga gtctgtttaa gtggcatcag
      601 atattggttt tccattacgc caacaccggc caggcgaagc tcttccattg cgcccttttt
      661 atcccctttc gccatttttt cattggcaat ttttatcgcg gcttcttttt cgggagttgc
      721 tacataactc tcagaaattc cgacagatgc attaatgaca atatattggt catcattgag
      781 attggtcttt ttacctggct tagcgaactt tgcccattcg gtactgtcat ctgacagcaa
      841 agcggaagct tcattggtca gttcttttgc cttttctgga tcgccatgaa acagagccag
      901 gcgcgctacc tgcacgtcac gcatcgcata taaaccttgt tcagagatac gctctgctgc
      961 tgccgttttc tgggtttgct gaacttgctg agtttgctgt tgtgccgcta ccggtgcatt
     1021 atcagcagcc catacagttg aagatgccag aattgctgtg accatcgtag ccataattaa
     1081 acgtttcata cttacctcat tctttaacag ttttagaaaa cgccgccatc atattgggtg
     1141 agcatgaaat gagaaatcag agagcaacgg tcattttgaa agggttatct tacagttgta
     1201 gttattatcg caagaagtat tttagtctta caattgtagg aatctgcttc ctgtcgcaga
     1261 gaactattct tatgtaccac aa
//
LOCUS       DAPHBJ010000375         1273 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.378, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000375 DAPHBJ010000000
VERSION     DAPHBJ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.378"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..587
                     /locus_tag="RHZ76_004577"
                     /pseudo
     CDS             1..587
                     /locus_tag="RHZ76_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(607..672)
                     /locus_tag="RHZ76_004578"
                     /pseudo
     CDS             complement(607..672)
                     /locus_tag="RHZ76_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
     gene            905..1009
                     /locus_tag="RHZ76_004579"
                     /pseudo
     CDS             905..1009
                     /locus_tag="RHZ76_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          371 a          279 c          294 g          329 t
ORIGIN      
        1 aaacatggca actacagttc acttacaccg cctctcagcc cggtaagcac cagaaaatca
       61 ttgatatggc catgaatggc gtcggatgtc gcgccagtgc acgcattatg ggcgttggcc
      121 tcaacacggt tttacgtcac ttaaaaaact caggccgcag tcggtaacct cgcgcataca
      181 accgggcagt gatgtgattg tctgcgctga aatggacgaa cagtggggct acgtcggtgc
      241 taaatcacgt cagcgctggc tgttttacgc gtatgacagg atacggagga cggttgtggc
      301 gcacgtcttc ggtgaacgca ctctggccac actggagcgt cttctgagcc tgctgtcggc
      361 ctttgaggtc gtggtatggg acggatggct ggccgctgta tgaatcacgc ctgaagggaa
      421 agctgcacgt tatcagcaag cgttacactc agcgcattga gcgacataat ctgaatctga
      481 gacaacatct ggcaaggctg ggacggaagt tactgtcgtt ctcaaaatcg gtggagttgc
      541 atgacaaggt catcgggcat tatctgaaca taaaacacta tcagtaagtt ggagtcatta
      601 cctcctcccg aactcaaagc tatcatacag ataattactg gttaacccta tatagggcat
      661 tttaaagtgt tgtgatctta cccagcaata gtggacacgc ggctaagtga gtaaactctc
      721 agtcagaggt gactcacaag acaaaacagt ttcaaccagt aaaaaaacgt aaacaccatt
      781 cgcatgaatt tcgtagttaa gatctgaagc ttgcagaatg catcggtgtt actgccggag
      841 tccgtgaact tagtctgcat gaatcaccac agctttacat ctgtcgcagt aaacagcaaa
      901 atcagcaaac ttacttaatt atatcgagtg cgattacaat cgctggcgat ggtacagttg
      961 gtgtggcggt ctcagtccgg aacaatttga aaatcagaat ctcacttaaa acggcatcca
     1021 ttttacaaaa gatggccgtc tgtgtatttg taatgaatac cttctacatg tttagagtgc
     1081 ataatccacg acaaggcgat atcctaaaaa ttagctagca ggcaactttg atggcataga
     1141 taatgttttt agtaaaaaag agttgactga atataataca gcctcttcct acataagaag
     1201 gaatgtgctc acaaaaaaaa ccattagtct gcttcctgtt cttacaataa tcagtattat
     1261 gtattttatt tat
//
LOCUS       DAPHBJ010000376         1248 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.379, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000376 DAPHBJ010000000
VERSION     DAPHBJ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.379"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..159
                     /locus_tag="RHZ76_004580"
     CDS             <1..159
                     /locus_tag="RHZ76_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2815065.1"
                     /translation="VEHPFRIIKRQFGIVKARYKGLLKNDNQLAMLFTLANLFRVDQM
                     IRQWERSQ"
     gene            complement(476..976)
                     /locus_tag="RHZ76_004581"
     CDS             complement(476..976)
                     /locus_tag="RHZ76_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HDV2815066.1"
                     /translation="MIASGIVQKLNAQMNQEFYTSNLYLQLSQRCSENSLNGTALFLR
                     HQAQNTVTQMMRMYEYIKQSGATPVLQEQHARCGEFSTLEDLFEQTVSDYQKRINALT
                     SLTEDAQAANDKSAINFLKRYRKEEIVDGTLLQIILDEVRSAKKAGINMQQTDHYLVG
                     VIDRYH"
BASE COUNT          326 a          291 c          270 g          361 t
ORIGIN      
        1 gtggagcacc cgtttcgcat catcaagcgg cagttcggca tcgtgaaagc cagatacaag
       61 gggctgctga aaaacgataa ccaactggcg atgttattca ccctggccaa cctgtttcgg
      121 gtggaccaaa tgatacgtca gtgggagaga tctcagtaaa aaccggaaat aacgccagaa
      181 atggtggaaa aaatagccta aataggctga ttcgatgtgt ttgcgggaaa aaaatcggcc
      241 cagatccgcg aaattttaat cagcgagtca gcttgggaag aaatgacctg cttattcgca
      301 ccttccttaa tataaagaca caagcagaga catttttggc acgatataaa aacgctaagt
      361 taaaaaatca ctatgacgtg ttgggtatca aatgttcact ggcataaacc tctgtttctg
      421 atgtaaaaca gattttctga ggaggcgtaa aacctcctcg tactgcgaaa ttgatttagt
      481 gatagcgatc gatcacgcca accagatagt ggtcggtttg ctgcatgttg atgcccgctt
      541 ttttagcgct gcggacttca tccagaatga tttgcaacag cgtcccgtcc acgatttcct
      601 cttttctgta tcgcttcagg aagttaatcg ctgacttatc gtttgctgcc tgggcgtctt
      661 ccgtcaaaga ggtcagcgca ttaatgcgct tctggtaatc gcttaccgtc tgctcaaaca
      721 gatcttccag cgtggaaaat tcaccgcacc gggcgtgttg ctcctgtaac accggcgtgg
      781 cgccgctctg tttgatgtat tcgtacatgc gcatcatttg cgtcaccgta ttttgcgcct
      841 gatgacgcaa aaatagtgcg gtgccattaa ggctattctc gctgcacctc tggctgagct
      901 gaagatacaa atttgaggta taaaactcct gattcatttg cgcattcagt ttttgaacta
      961 ttccgctagc gatcatagga cttccttatt tcttgtcatc tcagaaaacg gtcactcttc
     1021 gaaaaaacaa ccagaataaa agcgattctt tcgctttttt tattgcgcct ggttacgcag
     1081 tcatattcat tttaagtcgg gatgctcctt atttaaatat ccaaccctac tatcgcattt
     1141 ctatctggca ttattctgtg atggtgtttc gatattcaca ctccctgtgc caatcctttc
     1201 ttatcgacag taggaaactt cctccggcta ttctgctgaa cacgatta
//
LOCUS       DAPHBJ010000377         1238 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.380, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000377 DAPHBJ010000000
VERSION     DAPHBJ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.380"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            168..845
                     /locus_tag="RHZ76_004582"
     CDS             168..845
                     /locus_tag="RHZ76_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HDV2815067.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNRGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            917..1201
                     /gene="ppnP"
                     /locus_tag="RHZ76_004583"
     CDS             917..1201
                     /gene="ppnP"
                     /locus_tag="RHZ76_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDV2815068.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          312 a          271 c          316 g          339 t
ORIGIN      
        1 atattaaaca ataacaatcc attactggaa tcatttggaa tctttacatt atgccgtgca
       61 cgtctgctgc tacgcttttt gtcatttgta gcacaagtaa gtgtcagcag tggtgcttca
      121 cacttgcccg gtaattaacg acgaaagaaa agtaaggtgg atgaacaatg agtgcgtcgt
      181 tggcgatcct gaccatcggc attgtaccta tgcaggaggt tttgccgctc ctgacggaat
      241 acattgacga agataatatt tcccatcata gcctgctggg gaagttaagt cgtgaagaag
      301 tgatggcgga gtacgcgcca gaagcaggcg aagacaccat tctcacatta ttaaatgaca
      361 accagctggc ccatgtttcg cgtcgcaaag tggagcgtga cctgcaaggt gtggttgaag
      421 tactcgataa tcggggttac gacgtcatta tattaatgag tacggcaaac attagtagta
      481 tgactgcgcg taatacgatc tttcttgagc cgtcgcggat attgccaccg ctggtttcct
      541 ctattgttga agatcatcaa gtgggggtta tcgttccggt tgaggagatg ctgcccgttc
      601 aggcgcaaaa atggcaaatt ttgcagaaat cgccggtatt ttcattgggt aaccccattc
      661 atgattcaga acaaaaaatc attgatgccg ggaaagaatt actggcaaaa ggagctgatg
      721 tcatcatgct ggattgttta ggatttcacc aacgtcatcg cgatttactg caaaaacagc
      781 tcgatgttcc tgtcttgctg tctaacgtat tgattgcacg gctggctgcg gaattactgg
      841 tgtaattttg cgtgacagtc agcgcctctg gcccctatag tgaagtagat attcaactac
      901 cagacagggc cagtttatgc ttcaaagtaa tgagtacttt tccggcaaag tgaaatcgat
      961 cggcttttcc agcagcagca ctggtcgcgc cagtgtgggt gttatggttg aaggcgaata
     1021 caccttcagc accgctgagc cggaagagat gacggtaatc agtggcgcgc tgaatgtgtt
     1081 actgcctgac gcgaccgact ggcaggtgta tgaagccggt tcggtgttta atgttcccgg
     1141 tcacagtgag tttcatctgc aagttgccga acccacctct tatctgtgcc gctatctgta
     1201 attcctcgcc ttccccttga acgggagggc atttttct
//
LOCUS       DAPHBJ010000378         1223 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.381, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000378 DAPHBJ010000000
VERSION     DAPHBJ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.381"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(95..994)
                     /gene="lysO"
                     /locus_tag="RHZ76_004584"
     CDS             complement(95..994)
                     /gene="lysO"
                     /locus_tag="RHZ76_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HDV2815069.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT          394 a          276 c          306 g          247 t
ORIGIN      
        1 gcttaacgcg gggataaaag ggatttttca tgcaacttta agggagattg atttagcgca
       61 attttggcgg cagggatcta ccgccagaga ggtattacgc agagaaaaag gcgatgagga
      121 tcggcactaa caggctaaga ataaaaccgt gaacaattgc cgccgggacc atatccagcc
      181 cgccagtacg ttgaagaacg ggcagggtga aatccattga tgtggcaccg cataagccca
      241 gtgcagtaga gcggctgcgg cgaatcagcc cagggatcaa cataatagca atcagttcac
      301 gggccagatc attaaaaaac gccgcgctcc cgattaccgg gccaaaagat tcggtcaata
      361 aaataccgga aagagaatac cagccgaaac cggaggccat tgccagcgcg gtattgatgg
      421 ggagatcaag aataaaggcg ttaattaaac caccaattaa tgaactggca accaccacca
      481 cggcgacaat cattccccgg cgattaagga caatctgctt taaggtcatg ccattattgc
      541 gcaactgaat accgacgagg aaaagtagca gaattaacgt gtattcgctg gcttcggtcg
      601 cgtgttgtaa gaaagccagt ccactcagac caatggcaaa accaatcact actacgccgc
      661 acagttttag cgactccagc gccatcgcaa tacgcgacgg gagtttttct tgctgatggt
      721 gattgcgcca tggcaggcct cgctccagcc acatcagggc ggcaatatta catagtaaaa
      781 taacggtaat actgacagca gaataatgca gaatcgccaa caggttactg gcgaggttat
      841 cgagaaacgc cagactgata cccataaaaa agagaataag gtaaaccatc cagcttaata
      901 gctgattaat aacttttaat gcagcttttt ggcgaagcgg aatgaggtaa cccacaatca
      961 ggggaaccag aatgattaac agcccagaaa acatgaaaac ccagtccttg caaagatgaa
     1021 gtcgaaatgc gcgatgacac actactgaaa gcggaaggac gagtaaagtt gcaattaaaa
     1081 ggaaatgtta tgcataagga gcagtagagt attcgttttc atttaaagat attcttgcgc
     1141 tttaattaca aactgcaccg atgttggtgg cgtcaaaatc gccgaggcgt tccctgaagg
     1201 ccggggcagc ccacatggat gtg
//
LOCUS       DAPHBJ010000379         1216 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.382, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000379 DAPHBJ010000000
VERSION     DAPHBJ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.382"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            8..>1216
                     /locus_tag="RHZ76_004585"
     CDS             8..>1216
                     /locus_tag="RHZ76_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV2815070.1"
                     /translation="MTVGTLAIMNITAVVSLRFIPSEAEYGLGAIFYYTFAAIMFLVP
                     VAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYFPTVLTFGAVSVA
                     FIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSKWCGIIGTIIPAI
                     ILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAYAGMEMNAVHVND
                     LDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLLVAYDMLFEWAGV
                     PWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKTNRHGMGHHLMFV
                     QGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIYLRHSQPDTKRPY
                     RAPALFFWATL"
BASE COUNT          296 a          229 c          268 g          423 t
ORIGIN      
        1 taagaaaatg acagtgggga cactcgctat catgaatata actgctgtag taagtctacg
       61 atttatacct tctgaagcag aatacggctt aggtgccatt ttttactata cctttgctgc
      121 aattatgttt ttagtacctg ttgcctttgt tgcggctgag ctagctacaa catatccaca
      181 aaaagggggg gcatttcgat ggataggaga agcatttggg gctcgctggg gttttgtagg
      241 aatgcttatg acctggctac aagtcatccc ctactttcca acagtactga catttggtgc
      301 agtctcggtt gcatttattg atgttgatat gggggtggca gaatcaattg ccagtaataa
      361 atggtatatt ttatttttcg tactatttgt ttactgggga gctatttgtg ttgcattacg
      421 tggcgttggt attttctcgc aggtatctaa gtggtgcggt attataggga caattattcc
      481 agctattatc cttgttattc ttggtttctc ctatctgtgg ttcagcggta aaccagcggt
      541 gatcccatta ggttggggtg atcttttccc taatatagct aacttccaga atattgttct
      601 tgccgccagc atatttcttg cttatgcagg gatggaaatg aatgcggtcc atgttaacga
      661 tttagataat ccaactaaaa aatacccaat tgcaattacc atcgcttcta taggtactat
      721 tgcaatattc ctgctcagta ctttaggggt cgcctttatt attcctacag ataaaattag
      781 tctaactcaa agtttgttgg ttgcctacga tatgctgttc gaatgggctg gagtgccatg
      841 gcttggctct gtgatggcat ttatgctagc aattggcgta ctgggtgggg tggttacttg
      901 gattgcaggg cctaatacag gtgtattagc gatcgcgaaa gctggttatt tacctaagtt
      961 tttccaaaaa accaatcgtc atggaatggg gcaccatcta atgtttgtac aaggtataat
     1021 tgtctcagtt ctttctgtta cttttgtgat aatgccttca gttcaagcag cttttcagat
     1081 tttatcacag ttaacagtta tgctctattt agttatgtat atgctcatgt tcgctagtgc
     1141 tatctatcta cgacattctc aacctgatac aaaacgccca tatcgagccc ctgctttatt
     1201 tttctgggca acactt
//
LOCUS       DAPHBJ010000380         1206 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.383, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000380 DAPHBJ010000000
VERSION     DAPHBJ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.383"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            242..673
                     /gene="silE"
                     /locus_tag="RHZ76_004586"
     CDS             242..673
                     /gene="silE"
                     /locus_tag="RHZ76_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="silver-binding protein SilE"
                     /protein_id="HDV2815071.1"
                     /translation="MKNIVLASLLGFGLISSAWATETVNIHDRVNNAQAPAHQMQSAA
                     APVGIQGTAPRMTGMDQHEQAIIAHETMTNGSADAHQKMVESHQKMMGNNTVSTTVPS
                     TSYAAMNEHERAAVAHEFMNNGQSGPHQAMAEAHRRMINAG"
     gene            817..1167
                     /locus_tag="RHZ76_004587"
     CDS             817..1167
                     /locus_tag="RHZ76_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDV2815072.1"
                     /translation="MKIKNTLFVILMLSLPAISAEHSEMKMSDMHSSASSQEYMAGMK
                     NMHEKMMAAVNESNPDKAFAKGMIAHHEGAIAMAETELKYGKDPEMRKLAQDIIKAQK
                     GEIEQMNKWLDSHK"
BASE COUNT          360 a          279 c          279 g          288 t
ORIGIN      
        1 tgtaaatgac aaagatgtca ttagcctgtc atgcagcaaa cagaagccat tcgatataat
       61 tagtgcaact tatcaggaag gctggattgc ttcatcaata cccggcgtca gagtacgcca
      121 ggaaatgaat atccagccct cttccggaga aataaacgct gccgaacttg tttcagttat
      181 ggaactgaaa acaccggttg tacttccccg gacatcacta attcagaaat ggagagttat
      241 catgaaaaat atcgtattag catctttgct gggctttggt ttaatttcat cggcctgggc
      301 cactgaaacc gtgaatatcc atgatcgcgt caacaatgca caggctcctg ctcaccagat
      361 gcagtctgct gcggctcctg tcgggatcca ggggactgct cctcgtatga ccggtatgga
      421 ccagcatgaa caggccatta ttgctcatga gaccatgacg aacggctcgg cggatgcgca
      481 ccagaaaatg gtggaaagtc atcagaagat gatgggaaat aacacggtat ccacgaccgt
      541 cccgtcaacg tcttacgcgg cgatgaatga gcatgaaaga gcagcggttg cccatgaatt
      601 catgaataac gggcaatccg gcccacatca ggccatggcc gaagcgcacc gccgcatgat
      661 caatgcaggc tgaatgcgac ggtagtatcc gtgctgttac cttttccctg atagattttc
      721 ctgaaacaac gttgctacat ttttgccccc cgtcagaggg cttttttgtc tgactcagct
      781 acacttaacc ggtacctgtc tgaaaggaat tattatatga aaatcaaaaa cacccttttt
      841 gtaatattaa tgttatccct gccagccatt tctgctgaac attcggaaat gaaaatgtca
      901 gatatgcact catcggcatc atcacaggaa tatatggctg gcatgaaaaa tatgcatgaa
      961 aaaatgatgg ccgccgttaa tgaatccaat cccgataagg cctttgcaaa aggtatgatc
     1021 gcacaccatg aaggggcaat cgcaatggct gagaccgagc tcaaatacgg aaaagatccc
     1081 gaaatgagaa agcttgcgca ggacatcatt aaagctcaaa aaggtgaaat tgagcagatg
     1141 aataaatggc ttgacagtca taaataatga acactggagt gttgtcttgc gcaccctctt
     1201 agggta
//
LOCUS       DAPHBJ010000381         1146 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.384, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000381 DAPHBJ010000000
VERSION     DAPHBJ010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.384"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1146
                     /locus_tag="RHZ76_004588"
     rRNA            <1..>1146
                     /locus_tag="RHZ76_004588"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          308 a          256 c          359 g          223 t
ORIGIN      
        1 tgtttcggca agggggtcat cccgacttac caacccgatg caaactgcga ataccggaga
       61 atgttatcac gggagacaca cggcgggtgc taacgtccgt cgtgaagagg gaaacaaccc
      121 agaccgccag ctaaggtccc aaagtcatgg ttaagtggga aacgatgtgg gaaggcccag
      181 acagccagga tgttggctta gaagcagcca tcatttaaag aaagcgtaat agctcactgg
      241 tcgagtcggc ctgcgcggaa gatgtaacgg ggctaaacca tgcaccgaag ctgcggcagc
      301 gacgcttatg cgttgttggg taggggagcg ttctgtaagc ctgtgaaggt gtgctgtgag
      361 gcatgctgga ggtatcagaa gtgcgaatgc tgacataagt aacgataaag cgggtgaaaa
      421 gcccgctcgc cggaagacca agggttcctg tccaacgtta atcggggcag ggtgagtcga
      481 cccctaaggc gaggccgaaa ggcgtagtcg atgggaaaca ggttaatatt cctgtacttg
      541 gtgttactgc gaagggggga cggagaaggc tatgttgacc gggcgacggt tgtcccggtt
      601 taagcgtgta ggctggtttt ccaggcaaat ccggaaaatc aaggctgagg cgtgatgacg
      661 aggcactacg gtgctgaagc aacaaatgcc ctgcttccag gaaaagcctc taagcatcag
      721 gtaacatcaa atcgtacccc aaaccgacac aggtggtcag gtagagaata ccaaggcgct
      781 tgagagaact cgggtgaagg aactaggcaa aatggtgccg taacttcggg agaaggcacg
      841 ctgatatgta ggtgaagcga cttgctcgtg gagctgaaat cagtcgaaga taccagctgg
      901 ctgcaactgt ttattaaaaa cacagcactg tgcaaacacg aaagtggacg tatacggtgt
      961 gacgcctgcc cggtgccgga aggttaattg atggggttag cggtaacgcg aagctcttga
     1021 tcgaagcccc ggtaaacggc ggccgtaact ataacggtcc taaggtagcg aaattccttg
     1081 tcgggtaagt tccgacctgc acgaatggcg taatgatggc caggctgtct ccacccgaga
     1141 ctcagt
//
LOCUS       DAPHBJ010000382         1141 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.385, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000382 DAPHBJ010000000
VERSION     DAPHBJ010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.385"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(11..922)
                     /locus_tag="RHZ76_004589"
     CDS             complement(11..922)
                     /locus_tag="RHZ76_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDV2815073.1"
                     /translation="MHFLNLQVTQAELLSGLISVSQTVAQIVLVMTVAWLVSAVLSRA
                     ITRISKRTRTSKRSADEVKRIETSLRLIRFVSRLVVWALAVTISLSILGISVAPILTT
                     AGVSGIAVGFAAQSLIKDYFSGLVLLLEGQLRIGDIVDIGGVAGQVEEMTLRYVRLRQ
                     LNGDVVYVPNGAITNLTNKTTDYSMAVIDVGVAYRENLQEALNVMESVARELASETTW
                     STRILAEPEVFGVETLGDSSVILRLRLKVLPGEQWSIRREYLHRIKQRFDELGIEIPY
                     PHLTLYAGQDKDGKAPAFRIEAPAGAQ"
BASE COUNT          270 a          314 c          290 g          267 t
ORIGIN      
        1 tttggccgtg tcattgggca ccagccggag cttcgatccg aaaggcaggg gcttttccat
       61 ccttgtcctg gccggcataa agcgtcagat gcggataagg gatttcgatg ccaagttcgt
      121 cgaagcgctg ttttatcctg tgcagatatt cgcgccggat tgaccattgc tcacctggca
      181 agaccttgag gcgaagacgc aggataaccg aagagtcacc caacgtttcg accccaaaga
      241 cctctggctc ggctaatatt ctcgttgacc aggttgtttc cgaagctagc tcacgggcga
      301 cactctccat aacgttcaat gcctcctgaa ggttttcgcg ataggcgaca ccgacatcga
      361 ttacggccat cgaatagtcc gttgtcttat ttgtcagatt ggtgatggct ccgttgggca
      421 catagaccac gtcaccattg agttgacgca ggcgcacata gcgcaacgtc atctcttcga
      481 cttggcctgc cacaccgcca atatccacga tatccccgat gcggagttgc ccttccagca
      541 acaagaccaa acccgaaaaa tagtctttta tcaggctctg cgcagcaaac cccactgcga
      601 tgccgcttac ccccgcagtc gtcaggatcg gtgccacaga aatgccaagg attgagaggg
      661 aaatggtcac ggccaaggcc cacacgacca gccgactgac gaaacggatc agacgaagag
      721 atgtttcaat gcgtttgacc tcatctgcag atctcttgga agtgcgggtt cttttgctga
      781 tgcgtgtgat ggcccgcgaa agcactgcgg acacgagcca cgccacggtc atgacgagca
      841 caatctgtgc aactgtttgg gaaacggaaa taaggccgga taggagctct gcctgtgtga
      901 cctgcaaatt caaaaaatgc atttatatgc actccctatt attctggggt aagcggatta
      961 aatggttgat cttacctttg catgagagtg agacagaccg gcatctcatc ggattatctg
     1021 gttgatgctt ggcggcggca aaaactgagt gcaacacctg acctttccgg tacggtgctg
     1081 cccccatgtc ttataccgaa ccgctttttc ttccggcacg gattgccatt ccacctcagc
     1141 t
//
LOCUS       DAPHBJ010000383         1131 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.386, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000383 DAPHBJ010000000
VERSION     DAPHBJ010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.386"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            37..1125
                     /locus_tag="RHZ76_004590"
     CDS             37..1125
                     /locus_tag="RHZ76_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV2815074.1"
                     /translation="MITTLHIQNYRSIREMSLELEQLNIVFGPNGTGKSNIYKAIHLM
                     HSAAQGQFSQALANEGGILKVFWAGKTRSDQLRRMNLAVETETYEYELQVGFVEKLPY
                     PSQFQLDPVIKEESIWLSGQYRRPSSQLMRRKNQAVFLNNVHHEKVTHSGTLYENESV
                     FGQLGEPHLYPEVSQMRESLRNWRFYQEFSVSIGSAMRAPQVGFRSPVLASDGANLAA
                     AFQTIVEIGDELLLMRILDQAFPGCVFYSDNTGGRFRMMMQREGLSRPLEPAEFSDGT
                     LRFLCLAVALLSPRPPAFIALNEPENSLHPQMLPALASLIAEASRYSQIWLTSHSPEL
                     ANLIEKQRSFSLYQLSMVEGETKVERLG"
BASE COUNT          257 a          321 c          324 g          229 t
ORIGIN      
        1 ttatgtacag gattgtaacg caaaaaggga ctgcctgtga tcaccaccct gcacattcaa
       61 aactaccgct cgattcgcga aatgtcgctg gagctggaac agctcaacat cgtctttggc
      121 ccaaacggca ccggaaaatc caatatctac aaggcgatcc acctgatgca cagcgccgca
      181 caggggcagt tttcgcaggc actggcgaac gagggtggga ttttaaaagt gttctgggca
      241 ggcaaaaccc gcagcgacca gctgcggcgg atgaatttgg ccgttgaaac cgaaacctac
      301 gaatacgagc tgcaggtagg ctttgtcgaa aaactgcctt acccctcaca gtttcaactc
      361 gatccggtga tcaaagaaga gtccatctgg ctgagtggtc aataccgccg cccgtcgtca
      421 cagctgatga ggcgtaaaaa tcaggccgta tttctgaaca acgtgcatca cgagaaagtc
      481 acccacagcg gcacgctgta tgagaacgaa tcggtgttcg gccagcttgg cgagccgcat
      541 ctttacccgg aagtgtcgca gatgcgcgag tcgctgcgta actggcgctt ttatcaggag
      601 ttttctgtat caatcggctc ggccatgcgc gcgcctcagg tcggtttccg ctccccagtg
      661 ctggccagcg acggcgcaaa cctggcggcg gcgtttcaga ccatcgtgga aattggcgac
      721 gagctgctgc tgatgcgcat cctcgaccag gccttccccg gctgcgtgtt ttacagcgac
      781 aacaccggcg gacgcttccg gatgatgatg cagcgtgaag ggctaagcag accgctggag
      841 ccggcggaat tctccgacgg cactctgcgt tttctgtgcc tggcggtggc gctattaagc
      901 ccgcgtccac cggcctttat cgccctcaac gaaccggaaa acagcctgca tccgcagatg
      961 ctgcccgcgc tggcgagctt aatcgccgag gccagccgct actcgcaaat ttggctgacc
     1021 agccactcgc cggaactggc gaatttaatt gagaaacaga gatcgttttc gctgtatcag
     1081 ctgtcgatgg tggaagggga aacgaaggtg gagaggctgg ggtagtctcc c
//
LOCUS       DAPHBJ010000384         1101 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.387, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000384 DAPHBJ010000000
VERSION     DAPHBJ010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.387"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT          297 a          267 c          255 g          282 t
ORIGIN      
        1 taagggggtt aaatgccagg atacgcgacc agcattcccc atggtctggc gaataaggga
       61 gttaaatacc acggtacctg accagcatga ccccatgtcc tggcgattaa agggttaaat
      121 gccacgctac gcgaccagcc tccccatggc ctggcgatta agggggttaa atgccacggt
      181 acccgaccag cttgcctcca tggtctggcg attatggggg ttaaatccca cggtacccga
      241 ccagcaggca ccgtggtcat tggctaaagg ggaaattgcc tcggcttgca gacaggacaa
      301 cgtgacgccc tagcggttaa gtgggtttaa tacgacggta cacaataagt atcccaccag
      361 aatctgtcta ttgagtggat tagattaaaa gatattcggc ctggatctcg acttagaatg
      421 acgaaatatt tggcaaaaaa atagggtatt cggcaaatcc tgttgttagg acatcccgaa
      481 tacccaaaaa atatttggca atcaatatga attgcccata cccccaattg acaataagag
      541 agttttttgg caattcgtat aattaggcca attcatccgt gtcgtctatt gcactaacga
      601 agtattccgt gcggtagtca tctccccccc tcaactagac tgaaggtgat ccatcttttg
      661 caagcgtacg gtcacctgac atgctgtccg ctcgtacagg aattgtcatg cattctttaa
      721 tggtatctgc ttcagccacg tgtccagtac actggttttc aaatccagca actactacat
      781 acgcatccgg gtcgagcctt ttaagctggt ctataagctc ttttactttt atgggttacc
      841 tctacgtttt tgtatatttt atcacgctga acggaagggc gaataactgt agtgaaacaa
      901 aaaaagtttt ggccggaaaa ccttttagac tggctttcct ttagcaaaga actggtgaat
      961 aggggagcaa ttccgcgcta cgcgaccagc atcccatcgt gtacagacga gtaagtggtt
     1021 aaatggcctg gtacccggca ataatccctc catggtctgc cgattaaggg gttaaatgcc
     1081 acagtacccg accagcttcc c
//
LOCUS       DAPHBJ010000385         1099 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.388, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000385 DAPHBJ010000000
VERSION     DAPHBJ010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.388"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            79..1053
                     /gene="cysB"
                     /locus_tag="RHZ76_004591"
     CDS             79..1053
                     /gene="cysB"
                     /locus_tag="RHZ76_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HDV2815075.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT          287 a          259 c          265 g          288 t
ORIGIN      
        1 attattaaat cgtattagtc acccgccagg tgtgacgaaa aaacgatgtt ctgatggcgt
       61 ctaagtggat ggtttaacat gaaattacaa caacttcgct atattgttga ggtggtcaat
      121 cataacctga atgtctcatc aacagcggaa ggactttaca catcacaacc cgggatcagt
      181 aaacaagtca gaatgctgga agacgagcta ggcattcaaa ttttttcccg aagcggcaag
      241 cacctgacgc aggtaacgcc agcagggcaa gaaataattc gtatcgctcg cgaagtcctg
      301 tcgaaagtcg atgccataaa atcggttgcc ggagagcaca cctggccgga taaaggttca
      361 ctgtatatcg ccaccacgca tacccaggca cgctacgcat taccaaacgt catcaaaggc
      421 tttattgagc gttatcctcg cgtttctttg catatgcacc agggctcgcc gacacaaatt
      481 gctgatgccg tctctaaagg caatgctgat ttcgctatcg ccacagaagc gctgcatctg
      541 tatgaagatt tagtgatgtt accgtgctac cactggaatc gggctattgt agtcactccg
      601 gatcacccgc tggcaggcaa aaaagccatt accattgaag aactggcgca atatccgttg
      661 gtgacatata ccttcggctt taccggacgt tcagaactgg atactgcctt taatcgcgca
      721 gggttaacgc cgcgtatcgt tttcacggca acggatgctg acgtcattaa aacttacgtc
      781 cggttagggc tgggggtagg ggtcattgcc agcatggcgg tggatccggt cgccgatccc
      841 gaccttgtgc gtgttgatgc tcacgatatc ttcagccaca gtacaaccaa aattggtttt
      901 cgccgtagta ctttcttgcg cagttatatg tatgatttca ttcagcgttt tgcaccgcat
      961 ttaacgcgtg atgtcgttga tgcggctgtc gcattgcgct ctaatgaaga aattgaggtc
     1021 atgtttaaag atataaaact gccggaaaaa taatttcttg cgttattttc ggcacctttt
     1081 atgtagcgaa ggtgccgga
//
LOCUS       DAPHBJ010000386         1090 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.389, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000386 DAPHBJ010000000
VERSION     DAPHBJ010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.389"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(65..1009)
                     /gene="leuO"
                     /locus_tag="RHZ76_004592"
     CDS             complement(65..1009)
                     /gene="leuO"
                     /locus_tag="RHZ76_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HDV2815076.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          308 a          259 c          225 g          298 t
ORIGIN      
        1 ttctacattc aataaaaaat gcagaataaa ccagacattc atgtctgacc tattctgcaa
       61 tcagttagcg tttgcaaatt gagactaatt gctcttccat ccactgatgg cctttatcgc
      121 gcccggcagc ttcatgccag gagagataac aggttctgct gttttgtttt aacggcagcg
      181 gtaatacctg taattctaag gattcagcga actcttcagc cagccaacgc ggcgcaatag
      241 cgaccaaatg cgtttgcgac accacgctaa gtacgctcat cattgccatg ccctgatacg
      301 cgatactggc ttgcttatct accgtgtcat accaaggttg actaaatgac gcgaaacgat
      361 cgagcgaaac cgccgcatgt tgttcgttat aaacatcatg tttcagtaac gggcccttaa
      421 ttgttggatg atttttgctg gctaccagca ccatttcatc tttaaataat ggtacgctgg
      481 taaattcagg acgatggaag tcttcataac taatcacaaa ctccgtttcc tgataacgca
      541 gctgatgttc agtgttctga tttaatgaag acttgaacat aacatgtata tttggcgcaa
      601 tctgctcaat gtgattataa atctgcgagg tcagaatgct gtctaacggg ctgcaaacac
      661 aaagatgaaa tacacgttca ctgctcgcgg gttcaaaacc tgaaccaggc aattcatttt
      721 gtactagttg caatgcctga cgaactgaac caaaaagttg aaatgcgcga gcagtcggtt
      781 gaataccacg gccataacga acaaaaagct cgtcattaaa catcaccttc aggcgtgcaa
      841 cagcgttact gaccgcaggt tgcgacattc ccagaacatg agcggcacga gtaatgtttt
      901 gctcctgcat cacggcatcg aaaacggtta ataagttgag atcgaccatg cgtagctgtg
      961 gcttgcttaa ctccgccgtc tctggatgat ctgtttgtac ctctggcata cttaactcca
     1021 ctgtcacact taactccctt tcccttattg gaatgcagag aaataatcct cgagaatatg
     1081 atttatcatg
//
LOCUS       DAPHBJ010000387         1086 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.390, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000387 DAPHBJ010000000
VERSION     DAPHBJ010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.390"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..1086
                     /locus_tag="RHZ76_004593"
                     /pseudo
     CDS             1..1086
                     /locus_tag="RHZ76_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          388 a          221 c          205 g          272 t
ORIGIN      
        1 gaataaagca gcacagcgta gcattacagc gccagacagg atgggagaaa gaaaaacata
       61 ggtttatcaa cgagatacaa tgattgaaag aggtgataac agcggtgttt atcagtatgg
      121 tcgtgctgaa catttcaccc acattatatc tgacaaacct tccccaaaag ataaatatgt
      181 tgcatatgct attaacattc ctgactatga gctggcagcc gatgtatata atattaacgt
      241 gacgtcacct tccggacagc aagaaacatt taaaatatta atcaatccag aacatctacg
      301 gcaaacactt gagcgtaaat ctcttactgc tgttcagaaa tcacaatgtg aaatcatcac
      361 ccccaaaaaa cctggcgaag cgattcttca tgcttttaat gccacctacc agcagatcag
      421 agaaaatatg tctgaatttg cacgttgcca ttatgggtat atacaaatcc ctccagtgac
      481 aactttccgt gccgacggac cagaaactcc cgaagaagaa aaaggttact ggtttcacgc
      541 ttatcaaccc gaagatcttt gtaccatcca caatccaatg ggagatttgc aggattttat
      601 cgcattggtt aaagatgcta aaaaatttgg tatcgatatc attcctgatt atacctttaa
      661 ctttatggga atcgggggta gtggtaaaaa tgacctggat tatccctctg ctgatatacg
      721 agcgaagatc agtaaagata tagaaagtgg tatccctggc tattggcaag gtcaggtttt
      781 gattccattt actatagatc cagtaacaaa agaacgtaaa caaatccatc cagaagatat
      841 acatctcact gcaaaagact tcgaagcaag taaagataat atttctaagg atgaatggga
      901 aaacctccat gcattaaaag aaatcgttta aatggaatgc ctaaaacaac ccccaaaagt
      961 gaccaggtta ttatgctgca aaatcaatac gttcgtgaaa tgcgaaaata tggcgtacga
     1021 ggtttacgtt acgatgcagc aaaacactca aaacatgaac aaatagaaag atcaataacc
     1081 ccaccg
//
LOCUS       DAPHBJ010000388         1086 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.391, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000388 DAPHBJ010000000
VERSION     DAPHBJ010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.391"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..154)
                     /gene="lacA"
                     /locus_tag="RHZ76_004594"
     CDS             complement(<1..154)
                     /gene="lacA"
                     /locus_tag="RHZ76_004594"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="transfers acetyl group from acetyl-CoA to the
                     6-hydroxyl of galactopyranosides; exact physiological role
                     is unknown; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HDV2815077.1"
                     /translation="MNMSMTEKIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRVMTP"
     gene            complement(220..>1086)
                     /gene="lacY"
                     /locus_tag="RHZ76_004595"
     CDS             complement(220..>1086)
                     /gene="lacY"
                     /locus_tag="RHZ76_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HDV2815078.1"
                     /translation="EKVSRRSNFEYGRARMFGCVGWALCASIVGIMFTINNQFVFWLG
                     SGCAFILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFRQPKLWFLSLYVIG
                     VSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIMFFAPLIINRIGGK
                     NALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCFKYITSQFEVRFSA
                     TIYLVCFCFFKQLAMIFMSILAGNMYESIGFQGAYLVLGLVALGFTLISVFTLSGPGP
                     LSLLRRQVNEVA"
BASE COUNT          331 a          268 c          243 g          244 t
ORIGIN      
        1 ttggagtcat tacccttttt tcaacttctg atggatgcga gtgattgaac tcatacatta
       61 atgttttccc acgaagtctt ttttcaggta agccttcgca catatcggta aatagcttgc
      121 ctgcttttat tttttctgtc atcgacatgt tcatttttaa cattccgtcc tgataagttg
      181 gtcggataag gcgctcgcgc cgtatccgac attaatttct taagcgactt cattcacctg
      241 gcgacgcagc agagaaagtg ggccggggcc gctaagcgtg aacacggaaa ttaaggtgaa
      301 gcccagcgcc accagaccca gcaccaggta agcgccctgg aaaccgatgc tttcatacat
      361 attgcccgcc agaatagaca taaaaatcat cgccagttgc ttaaagaagc agaaacagac
      421 cagataaatc gtcgctgaaa aacgcacttc aaactggctg gtaatatatt taaagcagcc
      481 caccagcagg aacggtactt caaacatatg cagcgttttc agaataacca cttccagtgc
      541 tgaggtagcg aacgatgagc caataatacg tactgacata atagtgccag ccagcagcag
      601 ggcgtttttc ccaccgatgc gattaatgat cagtggcgca aaaaacataa tcgaggcgtt
      661 aagtaattcg cccattgtcg ttacgtagcc aaatacccgc gtaccctgtt caccggtagc
      721 aaagaacgaa gtaaagaaat tagcaaactg ttggtcaaaa acatcgtagg tgcaggaaac
      781 gccaataaca tacagtgaca aaaaccacag ttttggctgt ctgaacagtt ccagcgccag
      841 cttaaggcta aatgccgaat ggttggcacc taccgcattg gcaaccgtgg cagaagaggg
      901 cgcatccgtt ttggcgaaaa agagtaaaac ggcgaggatg aatgcacagc cagaacccag
      961 ccagaaaaca aactgattat tgatggtgaa catgatgccg acaatcgagg cacacagcgc
     1021 ccagccaaca cagccaaaca tccgcgcgcg accatattcg aaattactgc gacggctgac
     1081 tttctc
//
LOCUS       DAPHBJ010000389         1074 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.392, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000389 DAPHBJ010000000
VERSION     DAPHBJ010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.392"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(93..1007)
                     /locus_tag="RHZ76_004596"
     CDS             complement(93..1007)
                     /locus_tag="RHZ76_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2815079.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT          304 a          240 c          224 g          306 t
ORIGIN      
        1 ttttcttaca ggtgtaggct aatagccttc acaaaataat tatccgcgca attttcgtga
       61 atggagcgca tagactaagg tcaaaatcac aattattcat agataaaagt gacaccaatg
      121 actgactgga cggttccggc tgaaaccttg tttcctgttt gtccataggt tgcgcttaaa
      181 cccagaggta ctttgctttt atttactgtc cccagggaga cattttctcc cgtagccagt
      241 atttttccat tacgcattag agtgacaccc actcccgatg cttttgttgc atcaggtgct
      301 gtgttagcaa acacttgtcg gctactgtca gtggtggcac ccgaaagata aaatgagagc
      361 ttttgttcgc ttgagcaata tacgccaagg ggtatttctg cgcttccagg aaagtccggg
      421 agatccacgg tgacattacg actatcaacg gtgcagccgc ctgttggcat aacaacattg
      481 ttatttgaaa taatattcca cgtaaaatta cgcggatttc cgcttcctaa tgttgcaatt
      541 ttatacatat gaatacgggc aatgacttcc cccgctttta tgacaactcc accggccgcc
      601 ccaactgggg taatatagag ttttaacggt aatggcatcg gtgtcttatc accaatatcc
      661 aatacgttgg tatttgtcgt caacgggaag gggtaggtca cattattcca gtacagcgat
      721 cctttatatg actgtaggga gcctgcgaaa gctgagcctt gtaccaggtt tatatgatca
      781 gtgtcgtacc agccgccgta atcattccag caacttatat gctgagacaa gtctacaacc
      841 agattctggc ccggctgtat tacagggtcg agattaacat aaacagaggt tgtacctgct
      901 ccaatacttg acccgccatc gacattacaa ctgaaggcaa aaactttgcc tgccattaat
      961 agaagataaa taccgaacag gacctttata ctgattgttt tacccatgat atatcctaag
     1021 gttaaaaatt gatttaaaaa gaaggctaag aaaatcgaat cgacgttatt gcca
//
LOCUS       DAPHBJ010000390         1073 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.393, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000390 DAPHBJ010000000
VERSION     DAPHBJ010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.393"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..493
                     /locus_tag="RHZ76_004597"
     CDS             <1..493
                     /locus_tag="RHZ76_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HDV2815080.1"
                     /translation="KGDDKFITTDYLQQCPEFTWREPRRVLKSLTVQYDKVLYLIEDS
                     KDSRRAIGKYIDVWHYPDGHKELRLNDVQLPYSTYDRLSEVDPVAIVDNKRLGHVLEV
                     SRQVQRKRDNNRSQSIPSGDEPSRRRHAPSINKPQRSLNEEDMLEAIIKLQGSSEAIF
                     GKR"
BASE COUNT          326 a          221 c          250 g          276 t
ORIGIN      
        1 aaaaggggat gataagttta tcaccaccga ctatttgcaa cagtgccctg aatttacctg
       61 gcgtgagccc cgtcgggtat taaaaagcct tacagtacag tacgataaag tgctgtatct
      121 gattgaagac agcaaggaca gccgccgggc catcggtaag tatatcgacg tctggcatta
      181 tcctgacgga cataaggaac tgcgcctgaa tgatgtacag ctcccctatt ccacctacga
      241 ccggttgtcg gaagttgatc ccgtcgcgat tgttgataat aaaagactgg gccatgtgct
      301 tgaagtttcg cgccaggtcc agagaaaacg ggacaataat cgctcacaat cgataccttc
      361 tggtgatgag ccttcccgaa gacgtcatgc tccgagcatc aacaaacccc agcgctcgct
      421 gaacgaagaa gatatgcttg aagccataat aaagcttcag gggagctctg aggccatctt
      481 tggtaaaagg tgaacttgac gcaaatcaga aaggaaactc cggactcact gacctatggc
      541 gatatgtgca ctgtgttgca accatgtcct gagaagattt ctgggaaatg caattaactt
      601 caatgatatt atatacaaag gaaaaagtcg ggaagccggg gtaaagttcg ggtgtcacag
      661 aaccccgaac cgctgcgcgg tatctggaga taaaggccat tcatgaaaat ggcctagagg
      721 atagaagcaa aaaaattaac gagaaatgac caagttatag atcgagtcta aatttttgaa
      781 tacatcatcg acttcaaagt gatgtgcagt gtctctgtaa caggtcgttg tgaaagatag
      841 accgctaggt tttatcgcat tgattttatt gattttatca ttggttacat tatgcatgtg
      901 tcgtaaatat aatggatatg aaaacacagc ctggtcaaat gattcattcg cagctactac
      961 acgaaattca tcaagtgatt tgcgcagttc agttggacga tctttatgtt cgctcttttc
     1021 aagcattgaa ctcattaata actcagtgaa tatccatgga gattgggttc taa
//
LOCUS       DAPHBJ010000391         1052 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.394, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000391 DAPHBJ010000000
VERSION     DAPHBJ010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.394"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1052
                     /locus_tag="RHZ76_004598"
     CDS             <1..>1052
                     /locus_tag="RHZ76_004598"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDV2815081.1"
                     /translation="DLSINKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAPKNGQAVG
                     TNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELR
                     EIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGI
                     DIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEA
                     LPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAY
                     LADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFT
                     LGGEATDIVVMRIMCR"
BASE COUNT          225 a          288 c          302 g          237 t
ORIGIN      
        1 tggatttgtc gatcaataaa aactggatcg acaaagaaga atatccgcaa tccgcagcca
       61 tcgacctgcg ttgcgtaaat atggttgccg atctgtggca tgcgcctgcg ccgaaaaatg
      121 gtcaggccgt tggcaccaac accattggtt cttccgaggc ctgtatgctc ggcgggatgg
      181 cgatgaaatg gcgttggcgc aagcgtatgg aagctgcagg caaaccaacg gataaaccaa
      241 acctggtgtg cggtccggta caaatctgct ggcataaatt cgcccgctac tgggatgtgg
      301 agctgcgtga gatccctatg cgccccggtc agttgtttat ggacccgaaa cgcatgattg
      361 aagcctgtga cgaaaacacc atcggcgtgg tgccgacttt cggcgtgacc tacaccggta
      421 actatgagtt cccacaaccg ctgcacgatg cgctggataa attccaggcc gacaccggta
      481 tcgacatcga catgcacatc gacgctgcca gcggtggctt cctggcaccg ttcgtcgccc
      541 cggatatcgt ctgggacttc cgcctgccgc gtgtgaaatc gatcagtgct tcaggccata
      601 aattcggtct ggctccgctg ggctgcggct gggttatctg gcgtgacgaa gaagcgctgc
      661 cgcaggaact ggtgttcaac gttgactacc tgggtggtca aattggtact tttgccatca
      721 acttctcccg cccggcgggt caggtaattg cacagtacta tgaattcctg cgcctcggtc
      781 gtgaaggcta taccaaagta cagaacgcct cttaccaggt tgccgcttat ctggcggatg
      841 aaatcgccaa actggggccg tatgagttca tctgtacggg tcgcccggac gaaggcatcc
      901 cggcggtttg cttcaaactg aaagatggtg aagatccggg atacaccctg tacgacctct
      961 ctgaacgtct gcgtctgcgc ggctggcagg ttccggcctt cactctcggc ggtgaagcca
     1021 ccgacatcgt ggtgatgcgc attatgtgtc gt
//
LOCUS       DAPHBJ010000392         1040 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.395, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000392 DAPHBJ010000000
VERSION     DAPHBJ010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.395"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1040
                     /locus_tag="RHZ76_004599"
     rRNA            <1..>1040
                     /locus_tag="RHZ76_004599"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          257 a          243 c          328 g          212 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga cttggaggtt gtgcccttga ggcgtggctt
      361 ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa
      421 tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa
      481 gaaccttacc tggtcttgac atccacagaa ctttccagag atggattggt gccttcggga
      541 actgtgagac aggtgctgca tggctgtcgt cagctcgtgt tgtgaaatgt tgggttaagt
      601 cccgcaacga gcgcaaccct tatcctttgt tgccagcggt ccggccggga actcaaagga
      661 gactgccagt gataaactgg aggaaggtgg ggatgacgtc aagtcatcat ggcccttacg
      721 accagggcta cacacgtgct acaatggcgc atacaaagag aagcgacctc gcgagagcaa
      781 gcggacctca taaagtgcgt cgtagtccgg attggagtct gcaactcgac tccatgaagt
      841 cggaatcgct agtaatcgtg gatcagaatg ccacggtgaa tacgttcccg ggccttgtac
      901 acaccgcccg tcacaccatg ggagtgggtt gcaaaagaag taggtagctt aaccttcggg
      961 agggcgctta ccactttgtg attcatgact ggggtgaagt cgtaacaagg taaccgtagg
     1021 ggaacctgcg gttggatcac
//
LOCUS       DAPHBJ010000393         1039 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.396, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000393 DAPHBJ010000000
VERSION     DAPHBJ010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.396"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            39..548
                     /locus_tag="RHZ76_004600"
     CDS             39..548
                     /locus_tag="RHZ76_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDV2815082.1"
                     /translation="MTDITTPSVYVGTYHKYNCGSIAGAWLDLTDFDSSEEFYERCRE
                     LHANEADPEFMFQDWEGIPSDMASECHINWDFINGFKQAREEGNEAAFVAFVDLFNST
                     DFDLFRDAYMGEAKDEETFAEEYLNDSGLLNEIPESVARYFDIVAYARDLFIGDFSLH
                     DGHVFNMTC"
BASE COUNT          204 a          294 c          289 g          252 t
ORIGIN      
        1 cgaaaaattg ccggtatcgg cgctttaagg agtacaccat gactgatatt acgacccctt
       61 ctgtttatgt tggcacttac cataaataca actgcggaag cattgcggga gcctggctcg
      121 atctgaccga ttttgatagc tcagaggagt tttacgagcg ctgccgcgag ttacacgcaa
      181 atgaggctga tccagaattt atgttccagg attgggaggg tattccgtct gatatggcgt
      241 cagagtgcca tatcaactgg gattttatta acggctttaa acaagcccgc gaggaaggaa
      301 acgaagcggc gtttgtggcc tttgttgatc tgtttaatag cactgatttc gacctgttta
      361 gagatgccta tatgggcgaa gctaaggacg aagaaacttt tgcagaggaa tacctgaatg
      421 atagcggtct actgaatgaa attcctgaat ccgtagcccg atattttgac atcgtagcct
      481 atgcgcggga tctgtttatt ggggatttca gtttacatga cggacacgta tttaacatga
      541 cttgctgaaa cctagtcccc cgccacgcgg gggatattaa acaccgctct aattaatcgc
      601 cggtatcggc aaacagccat ctgggcggct gcgccgcccc tttgctgctg cttgccggtg
      661 atctctacag ctcatgttct gccattgcgc taaatcctga tgccacaccg ggcgttaccc
      721 cttcggggtc gctctgctgt gctgtgcatc gagccggttc ccgtctcgcc ccgcttacgc
      781 gggcagcggc cgctaacgcc tccggccttc ggcctccgcg ctccgcttgg caggccacac
      841 cctgcgccgg actcgcccgg ccgcaagcgg ccgccccgcc ccttcggggt gccaacgccc
      901 ttcaggcgtc ggcatccttt gccagtgcgc tattgtgtta tccgtggatt gtgcagctca
      961 gtggggcgct ggccgtgacg gtgcggtgtc ccccgtaacc ggccgcgcgg cggccgctaa
     1021 ctcgcagtac ggcgccgcg
//
LOCUS       DAPHBJ010000394         1014 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.397, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000394 DAPHBJ010000000
VERSION     DAPHBJ010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.397"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(57..653)
                     /locus_tag="RHZ76_004601"
     CDS             complement(57..653)
                     /locus_tag="RHZ76_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV2815083.1"
                     /translation="MIIGYARKSTHLQDVTHQVDELTKAGCEQIYHEQISRGGTKRAK
                     NGAPELENCLKALREGDTLVVWALDRLGGSLSQVITLLDDLKKRGITFIAIKDRIDTS
                     APVIGEIYTHLMAIFSNFERNRNIERTRSGLAAARARGRVGGRKPSLSEDDIKQMKIL
                     LADPEMTVGAVAKRFNVSRMTIYRYTTKGNHSEKINDE"
     gene            892..>1014
                     /locus_tag="RHZ76_004602"
     CDS             892..>1014
                     /locus_tag="RHZ76_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_143212653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HDV2815084.1"
                     /translation="MFMSVFHNWLLEIACENYFVYIKRLSANDTGATGGHQVGLY"
BASE COUNT          250 a          235 c          224 g          305 t
ORIGIN      
        1 ggcggcacat cgcattcgac gaaacccaac ctaaccggag tcatcaggct agaatgttat
       61 tcatcattta ttttctctga atggttgcct tttgtcgtgt agcgataaat agtcatgcga
      121 gaaacattga agcgcttcgc aacagctcct actgtcattt caggatcagc aagcagaatt
      181 ttcatttgct taatatcatc ttccgaaagt gacggttttc ttccccctac acggcccctt
      241 gcgcgagcag ctgcgagtcc agatctcgtt cgttcaatat tgcggttgcg ttcaaagtta
      301 gagaatatag ccatcagatg agtatagatt tccccgataa caggagcgct agtatctatt
      361 ctgtctttga tggctataaa ggtgattccg cgctttttca ggtcgtcaag caacgtaatc
      421 acctggctca atgaaccgcc gagccgatca agcgcccaaa caaccagggt atcaccttcg
      481 cgcaatgcct tcagacagtt ctccagttcc ggggcaccat ttttggcgcg tttagtgccg
      541 ccgcgtgaga tctgttcatg atagatttgc tcgcaacccg cttttgtcag ttcatcaacc
      601 tgatgcgtta catcctgaag atgtgtcgat ttacgcgcat agccgattat cattttgcca
      661 ccctattttg ttacatcgtt tttgttgtat tgatactgta acgggttttg ttacagccgg
      721 agagcgtttt attgtatagg caactgctta tagggatgtg tcacagaaac catcgttttt
      781 gatacattaa tttaaccaat aggttgcaga tcaaatcgcc tgtaacagcc tttctggctg
      841 tttgtatata attatgaaaa aatggtgagt agagtttcag ggtaacaggg gatgtttatg
      901 tcggttttcc acaactggct actcgagatc gcatgtgaga attacttcgt ctacatcaaa
      961 cgcctttccg ccaacgatac cggcgcaaca ggtggtcacc aggtaggact ttat
//
LOCUS       DAPHBJ010000395         1011 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.398, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000395 DAPHBJ010000000
VERSION     DAPHBJ010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.398"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            94..>1011
                     /locus_tag="RHZ76_004603"
     CDS             94..>1011
                     /locus_tag="RHZ76_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDV2815085.1"
                     /translation="MQVNKFTDDIDISSHERFIAARKSTWVSVLVNCFLTSGQVITGI
                     FSGSQGLIADGIHSLSDLVSDFVVLIANHKSKKNPDDDHHYGHHRYENGASLILGTIL
                     FIVGIGMLWSAVNKMKQPDDIPQVHIVALWVALAALVIKELLFRYMLAVATRVKSTML
                     VANAWHARSDAASSLVVAIGIIGNLSGFKLLDPVAAMVVGLIVTRMGYSFMSDSLHDL
                     MDRSVDIETENKIKTTLLSTPGVEGIHDLKTRKMGDLVVVDVHLEINGNLSVKVGHDI
                     AVLARKSVLDNHHVLNVMTHVDPFFKEVSG"
BASE COUNT          273 a          178 c          224 g          336 t
ORIGIN      
        1 atctcaccaa ttattatatt ttcgccacag aacctgtccg ggaaggatag tccgtttagt
       61 attaaccgtt gatgctgtta taaggagttt ctgatgcagg taaacaaatt tacggatgat
      121 attgatatta gttctcatga gcgtttcatc gcggcccgta aaagtacgtg ggttagtgtg
      181 cttgttaatt gttttctgac atccgggcag gtgattactg gtatattttc tggatctcag
      241 ggacttatcg cggatggtat tcattccctg tctgatttag tctcagactt tgttgtactc
      301 attgcaaacc ataaaagtaa aaaaaatccc gatgatgacc atcactacgg tcatcatcgt
      361 tatgaaaatg gtgcatcatt aattctgggg acaattttat ttattgttgg catcggcatg
      421 ttatggtcag cagtaaataa aatgaaacaa ccagatgata ttcctcaggt acatattgtt
      481 gctctgtggg tggctttagc cgcactggtt atcaaagagc ttctttttcg ctatatgctt
      541 gccgtagcaa cccgagtaaa atccactatg ctggtcgcta atgcctggca tgcacgctct
      601 gatgccgctt cgtctttggt tgtggctatt ggtattattg ggaacttatc aggttttaaa
      661 ttactcgacc cagttgctgc aatggttgtc ggactgattg taaccagaat gggatattca
      721 tttatgtcag attcgcttca tgacttgatg gacagatccg tggatattga aactgaaaat
      781 aaaataaaaa ccacattatt gtcaacgcct ggagttgaag gtatccatga tctcaaaacc
      841 cgtaaaatgg gagatcttgt tgttgttgat gttcatctgg aaattaatgg taatttatca
      901 gtgaaagtag ggcatgacat tgctgtttta gccaggaaat cagttttaga taatcatcat
      961 gtattaaatg tcatgacgca tgttgaccct ttttttaagg aggtaagtgg c
//
LOCUS       DAPHBJ010000396         1004 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.399, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000396 DAPHBJ010000000
VERSION     DAPHBJ010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.399"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(133..288)
                     /locus_tag="RHZ76_004604"
     CDS             complement(133..288)
                     /locus_tag="RHZ76_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815086.1"
                     /translation="MREYPNGEKTHLTVMAAGFPSLTGDHKVIYVAADRHVTSEEILE
                     AAIRLLS"
     gene            complement(368..433)
                     /locus_tag="RHZ76_004605"
     CDS             complement(368..433)
                     /locus_tag="RHZ76_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815087.1"
                     /translation="MKAPSGAFLLGVYSMDTHILR"
     gene            complement(436..624)
                     /locus_tag="RHZ76_004606"
     CDS             complement(436..624)
                     /locus_tag="RHZ76_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDV2815088.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDMNPFGAKCIFCKSMMITF
                     DNISQYLNASRLSLDLKK"
     gene            complement(635..847)
                     /gene="cspI"
                     /locus_tag="RHZ76_004607"
     CDS             complement(635..847)
                     /gene="cspI"
                     /locus_tag="RHZ76_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HDV2815089.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
BASE COUNT          348 a          197 c          174 g          285 t
ORIGIN      
        1 agtaagtcga ttatatggtg caaacgtgta aaagataaga tgaaacatcg caataatcaa
       61 catacgatag tctaaatttt acacaaacag acaaagagaa ttttcctgaa ttatcaatgc
      121 aatagcatca aatcaactca agagccttat tgctgcttcc agaatttctt ctgaagtaac
      181 atgtcgatcc gcggctacat aaatgacttt atgatctccg gtcagagatg gaaaccctgc
      241 ggccattaca gtaaggtgtg ttttttcgcc atttggatat tcacgcatga tggtgttaac
      301 tccagtcatc gctggcacta ccactgctgg ttcagagtta aaaaaaacta tgattttttt
      361 catgatgtta ccgtagtatg tgagtatcca tcgaatagac accaagcaaa aaagctcccg
      421 aaggagcctt cattttcact tttttaaatc caacgacaga cggctggcat ttaagtattg
      481 tgaaatatta tcaaatgtaa tcatcattga tttacaaaag atacattttg ccccgaaagg
      541 attcatgtca gaaacatcaa aagatgatgt tctatactgg gaaccatgac aacacgggca
      601 tctaaagtga atatggtttg taatattgtc tacctcaaag cgccactaca tgaacagcgg
      661 caggaccttt aggtccgttc tcaataccaa attcaacttc ctgattctca gttaatgttt
      721 tgaaatcgtt gctctgaatt gctgagaaat ggacaaacac atctttgctg ccatctttcg
      781 gcgtgatgaa accaaaacct ttttcagggt taaaccattt cactaaacca gtcattttgt
      841 tagacataat tattaccttt tgaagaaatt agcccttggg cagaatggtc cgaaaaaaaa
      901 tatcagagag aaaaaccaac aaggaaatct caagaggtac aaataataaa attataacaa
      961 tgactgcttc agataaattt gtaacaaacc agaacaccat taac
//
LOCUS       DAPHBJ010000397          972 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.400, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000397 DAPHBJ010000000
VERSION     DAPHBJ010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.400"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            128..241
                     /locus_tag="RHZ76_004608"
                     /pseudo
     CDS             128..241
                     /locus_tag="RHZ76_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611474.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(467..>972)
                     /locus_tag="RHZ76_004609"
     CDS             complement(467..>972)
                     /locus_tag="RHZ76_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDV2815090.1"
                     /translation="YSTKPLEDAENGLSSRMRRTIAELYDLFNDLERRIHFFDKEIET
                     VFRQSEACQRIAKVKGIGPKTATAVVAAIGKGTEFKNGRHFAAWLGLVPRQHSSGDKH
                     LRTLFIHGARAVVRVATNNNDGHMNQWVNQLKERRGFNKTTVAVANKNARIIWSMLRN
                     ETEYQVV"
BASE COUNT          247 a          246 c          205 g          274 t
ORIGIN      
        1 aatttttaaa cattaattta ttcttaacag aatatgaaaa aattgacagt taccactgac
       61 ctgctccccg ttgattagta caccccgatg ttagtaatgt cttcataagc cacatgagga
      121 catccccatg aagaagcgtt tttccgacga acagatcatc agtattctcc gcgaagccga
      181 agctggggta cccgcccgtg aactctgccg caagcatgcc atttccgatg ccacgtttta
      241 catctatgga ctacctccgt tttgcaagta ctgaatctgg ttttgggttg ttgcttacat
      301 ctatccggca tcaggaaaat ctgtgcccaa atgggtaatc cgcacataat cgcctcaaca
      361 actggacggc ctctgaggcc agtataaaaa tcaggttccg attgtgcagg tgcaacctgt
      421 caccatttct cagtacgctg caactttttc tggcagggga ttaatttcac actacctgat
      481 actcggtctc atttctcagc atcgaccaga ttattctcgc gtttttgtta gcgaccgcca
      541 cggtcgtttt attaaatccg cgccgttcct ttaactggtt aacccactga ttcatatggc
      601 catcattgtt attcgtggca accctgacga cagcgcgggc accatgaata aaaagtgtcc
      661 gcagatgctt gtcgccactc gaatgctggc gtggaaccag acccagccat gcagcaaagt
      721 ggcggccatt cttaaattca gttcctttgc caatagcagc aacaacggcc gtggccgttt
      781 tagggccaat gcctttaact ttggcgatac gctgacaggc ttctgattgc ctgaatactg
      841 tttcaatttc cttatcaaaa aaatggatcc gacgctcgag atcgttaaag agatcataga
      901 gttcggcaat tgttctgcgc atacgggaac ttagaccgtt ttctgcatct tcaaggggct
      961 ttgttgaata aa
//
LOCUS       DAPHBJ010000398          963 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.401, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000398 DAPHBJ010000000
VERSION     DAPHBJ010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.401"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>963)
                     /gene="trkG"
                     /locus_tag="RHZ76_004610"
     CDS             complement(<1..>963)
                     /gene="trkG"
                     /locus_tag="RHZ76_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Trk system potassium transporter TrkG"
                     /protein_id="HDV2815091.1"
                     /translation="LWIDSELNLTFIDALFEGVSGITTTGATVIDDVSSLPRAYLYYR
                     SQLNFIGGLGVIVLAVAVLPLLGIGGTKLYQSEMPGPFKDDKLTPRLADTSRTLWITY
                     SLLGIACIVCYRLAGMPLFDAICHGISTVSLGGFSTHSESIGYFNNYLVELVAGSFSL
                     LSAFNFTLWYIVISRKTIKPLIRDIELRFFLLIALGVIIVTSFQVWHIGMYDLHGSFI
                     HSFFLASSMLTDNGLATQDYANWPTHTIVFLLSSSFFGGCIGSTCGGIKSLRFLILFK
                     QSKHEINQLSHPRALLSVNVGGKIVTDRVMRSVWSFFFLYTLFTV"
BASE COUNT          352 a          215 c          167 g          229 t
ORIGIN      
        1 accgtgaaga gagtataaag aaaaaagaaa ctccatacag acctcattac acgatctgta
       61 actatcttcc ctcctacatt tacactcaac aacgctctgg gatgagaaag ctgatttatc
      121 tcgtgtttgc tttgtttgaa aagtataaga aatcgaagtg acttaattcc accacaagtt
      181 gaacctatac atcccccaaa gaaacttgac gacagcaaaa acactatcgt gtgcgtgggc
      241 cagtttgcat aatcctgcgt agctaaacca ttatcagtga gcatggagct ggcaagaaaa
      301 aacgaatgaa taaaacttcc atgcaagtca tacataccta tatgccagac ctggaaagag
      361 gtaacaatga tcacccctaa ggctattaac agaaagaaac gaagttcaat atctctgatt
      421 aaaggtttta tcgttttcct gctaataaca atataccaaa gagtgaagtt gaaagccgat
      481 agcagggaaa aagaaccagc caccagctca accaaatagt tattaaaata tccgatactc
      541 tcgctatgag ttgagaaacc accaagcgaa actgtggaaa tcccgtgaca aatagcatca
      601 aacaaaggca ttcctgcaag tctataacag acaatacaag caatacctaa taaagaataa
      661 gttatccaca gtgtccgtga cgtatcggcc aggcggggag tgagtttgtc atccttaaat
      721 ggccccggca tttctgactg ataaagcttt gtaccaccaa tacccaataa tggcaataca
      781 gcaaccgcca gaacaataac tcctaaacca cctataaaat ttaactgtga ccgatagtac
      841 aaatatgccc gaggtaatga actaacatca tcaattacag ttgctcctgt tgttgttatt
      901 ccagaaaccc cttcaaacag agcatcaatg aacgttaaat taagttctga gtcaatccat
      961 aaa
//
LOCUS       DAPHBJ010000399          954 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.402, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000399 DAPHBJ010000000
VERSION     DAPHBJ010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.402"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(1..589)
                     /locus_tag="RHZ76_004611"
                     /pseudo
     CDS             complement(1..589)
                     /locus_tag="RHZ76_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068158.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          334 a          196 c          138 g          286 t
ORIGIN      
        1 tcacattaaa aatgtaacca tcaaccgtat cgcttacagt gttaatacac tgaaagttaa
       61 ttcaattagc atgatcatca acaaaaacgt cgaaattatt cctttcaaaa aaaggatttt
      121 tcagattttg atcaccttta aaaacagtcg ttaactcctg gaccgaatgt atatatgaag
      181 catcagcagt atgttctaac ttatcattaa caaaaggact gaaatcactt ttaacagatg
      241 ggatagagaa tccttttaag ctatttaaaa caggaagaga aatctcacta tggcttgcta
      301 gccaaaacat tgtataacat gcaaccctgt cactatcttt catccaaata aaataaacct
      361 caggaacaat agtcgcagag gcatatgcct tcatatcacg aacgattatt ttccttcgtt
      421 ctcttaacga gtcattatca gcaataagtt tattgatcaa ataagcatat tgagtcgcag
      481 agtgaaaaag tctcacttct tcccagttca ccttttcttt gtagagcgtc tcaaggcatc
      541 tcagatactg atagtacttc ctacaacgac cataatccat ctgcgccatc caaatccccc
      601 acgaaaatca gcacaaaaaa cgcacaatga agcgtaatta tgtcataaaa atagaataat
      661 aaaaaactaa tttattagta attttttagt aatgttattt taatatttcc tttgatattc
      721 attgcaatag agccactata ttgaagttaa aacccatcaa aagatgtgtg caatcaagcg
      781 acgcgtaatt ttttacatta ctaggttatg gtataagccg ctccctcctc catacatagc
      841 caatttttgt tcgtatgagt tgccaaggtt tccagtttgc acaacagctt caaatgcagc
      901 acagatactt caacatcaaa cagcatcgta gaatcagtgc tagtgacgac tgtt
//
LOCUS       DAPHBJ010000400          950 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.403, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000400 DAPHBJ010000000
VERSION     DAPHBJ010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.403"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>950
                     /locus_tag="RHZ76_004612"
     CDS             <1..>950
                     /locus_tag="RHZ76_004612"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815092.1"
                     /translation="TNIKNIIAEQTKASEVEIYSLDEIKSVQLNGNGYHFLITDKYAD
                     DGLNNALKVIISYLVPAGRYVSFHTDKTVVETLSNYNLQPEVYLFYEHGLLKTQIHQG
                     EEITLSPELCVFMKSPLARSELPYQETIYGYSHPPKNLLAFARDYTNPWLIRGIVEFP
                     FRNRSTYHLQQYSHQILEHSAPDSPDYAAALAVLGYQMLSGSDDTADIYAKMLDYCSN
                     VSQMDNPTPHQYRWLISLSTLLGLICNKNNDKANALIHLSRAANSSIDKFSPSIGTKI
                     LQSFYLQSVILISLNRISCAEIIVDRGIKRGIQLLYQHPD"
BASE COUNT          304 a          188 c          169 g          289 t
ORIGIN      
        1 acaaacatta aaaatattat tgcagaacaa acaaaggcat ctgaagttga aatttactca
       61 ttggatgaaa ttaaaagtgt tcagctaaat ggcaatggct accattttct cattactgat
      121 aaatatgccg atgatggact aaataatgca ctcaaagtca ttatatctta cctggtgccc
      181 gctggcaggt acgttagttt tcatacagat aaaactgtag ttgagacatt aagtaattac
      241 aatttacaac ctgaagttta tttattttat gagcatggcc ttttaaagac tcaaatacat
      301 cagggagagg aaattacact ctctcctgaa ttatgtgttt ttatgaaaag ccctttagcc
      361 aggtcagagc ttccgtatca ggaaacgatt tatggctact cgcatccacc aaaaaaccta
      421 ttagcttttg ccagagatta tactaacccg tggcttattc gcgggatcgt agaattccca
      481 ttcaggaata ggtcaactta tcatctacag cagtatagcc atcagatttt ggagcacagt
      541 gctcctgaca gccccgatta tgccgctgca ttggcagtat taggttatca aatgctaagc
      601 ggcagtgatg atacagctga catttacgca aaaatgctag attactgctc aaatgtgtcg
      661 cagatggata atcctacgcc acatcaatat cgttggctca tatctttatc gacactttta
      721 ggtcttattt gtaacaaaaa taatgataaa gctaatgcgt taatccattt atctcgtgca
      781 gcaaactcca gcattgataa atttagcccc tctatcggca cgaagatttt acaaagcttt
      841 tatcttcagt ccgttatatt aatttcttta aatagaatct cctgtgccga gataatcgtc
      901 gatagaggga ttaaaagagg tatacagcta ctttatcaac atcctgacga
//
LOCUS       DAPHBJ010000401          946 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.404, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000401 DAPHBJ010000000
VERSION     DAPHBJ010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.404"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..821
                     /locus_tag="RHZ76_004613"
     CDS             <1..821
                     /locus_tag="RHZ76_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5-like element IS5 family transposase"
                     /protein_id="HDV2815093.1"
                     /translation="YPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSA
                     LPDRTTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNK
                     EQQRDPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEE
                     QFVSADAGYQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKNKTAINIESMKASIR
                     AKVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQWERSH"
BASE COUNT          265 a          256 c          252 g          173 t
ORIGIN      
        1 cttatccgct ggaaaccatg ctacgcattc actgcatgca gcattggtac aacctgagcg
       61 atggcgcgat ggaagatgct ctgtacgaaa tcgcctccat gcgtctgttt gcccggttat
      121 ccctggatag cgccttgcct gaccgcacca ccatcatgaa tttccgccac ctgctggagc
      181 agcatcaact ggcccgccaa ttgttcaaga ccatcaatcg ctggctggcc gaagcaggcg
      241 tcatgatgac tcaaggcacc ttggtcgatg ccaccatcat tgaggcaccc agctcgacca
      301 agaacaaaga gcagcaacgc gatccggaga tgcatcagac caagaaaggc aatcagtggc
      361 actttggcat gaaggcccac attggtgtcg atgccaagag tggcctgacc cacagcctgg
      421 tcaccaccgc ggccaacgag catgacctca atcagctggg taatctgctg catggagagg
      481 agcaatttgt ctcagccgat gccggctacc aaggggcgcc acagcgcgag gagctggccg
      541 aggtggatgt ggactggctg atcgccgagc gccccggcaa ggtaagaacc ttgaaacagc
      601 atccacgcaa gaacaaaacg gccatcaaca tcgaatccat gaaagccagc atccgggcca
      661 aggtggagca cccatttcgc atcatcaagc gacagttcgg cttcgtgaaa gccagataca
      721 aggggttgct gaaaaacgat aaccaactgg cgatgttatt cacgctggcc aacctgtttc
      781 gggcggacca aatgatacgt cagtgggaga gatctcacta aaaactgggg ataacgcctt
      841 aaatggcgaa gaaacggtct aaataggctg attcaaggca tttacgggag aaaaaatcgg
      901 ctcaaacatg aagaaatgaa atgactgagt cagccgagaa gaattt
//
LOCUS       DAPHBJ010000402          946 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.405, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000402 DAPHBJ010000000
VERSION     DAPHBJ010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.405"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..759
                     /locus_tag="RHZ76_004614"
     CDS             <1..759
                     /locus_tag="RHZ76_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS domain-containing protein"
                     /protein_id="HDV2815094.1"
                     /translation="VEQMQNQMDPVYTPARKIHLYHCDHRGLPLALISKEGTTEWCAE
                     YDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKG
                     GWNFYQYPLNPVTNTDPLGLEVFPRPFPLPIPWPKSPAQQQADDNAAKALTKWWNDTA
                     SQRIFDSLILNNPGLALDITMIASRGNVADTGITDRVNDIINDRFWSDGKKPDRCDVL
                     QELIDCGDISAKDAKSTQKAWNCRHSRQSNDKKR"
     gene            780..>946
                     /locus_tag="RHZ76_004615"
     CDS             780..>946
                     /locus_tag="RHZ76_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HDV2815095.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSA
                     RVLQLRGGQDAV"
BASE COUNT          304 a          198 c          230 g          214 t
ORIGIN      
        1 gtggagcaga tgcaaaacca gatggacccg gtgtacacgc cggcgcgaaa aatccacctg
       61 taccactgcg accatcgcgg cctgccgctg gcccttatca gcaaggaagg gacaacagaa
      121 tggtgcgcag aatacgatga atggggcaac ctgctgaatg aagagaaccc gcatcagctg
      181 cagcagctta tccgcctgcc ggggcagcag tatgatgagg agtccggcct gtattacaac
      241 cgccaccgct attatgaccc gctgcagggg cggtatatca ctcaggatcc gattgggctg
      301 aaggggggat ggaattttta tcagtatccg ttgaatccag ttacgaatac agatcctctg
      361 gggttagaag tttttcctag accattcccc ttgccaattc catggcccaa aagccctgca
      421 cagcagcaag cagatgataa tgctgcaaaa gcattgacaa aatggtggaa cgatacagca
      481 tcacaaagaa tatttgactc tctaatattg aataatccgg gactagcatt agatataaca
      541 atgatagctt ctcgtggaaa tgttgcagac acagggataa ctgatcgtgt caatgacata
      601 ataaatgaca gattctggag tgatgggaaa aaacccgaca gatgtgacgt acttcaggaa
      661 ctaattgatt gtggtgatat tagtgctaaa gatgcaaaaa gcacacagaa agcctggaat
      721 tgtcgtcact ccagacagtc aaacgataaa aaaagatagc ccttgtggag gttcctgcaa
      781 tgtcaaatac ataccagaaa agaaaggcaa gtaaagagta tggtttatat aatcaatgta
      841 agaaactaaa tgatgatgaa ttatttcgct tacttgatga tcacaattcc ttgaaaagga
      901 tttcatctgc cagagtatta cagttaagag gtgggcaaga cgctgt
//
LOCUS       DAPHBJ010000403          945 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.406, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000403 DAPHBJ010000000
VERSION     DAPHBJ010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.406"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(129..722)
                     /gene="rclC"
                     /locus_tag="RHZ76_004616"
     CDS             complement(129..722)
                     /gene="rclC"
                     /locus_tag="RHZ76_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HDV2815096.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(734..>945)
                     /locus_tag="RHZ76_004617"
     CDS             complement(734..>945)
                     /locus_tag="RHZ76_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV2815097.1"
                     /translation="TLLSGVMAFSTNADDKIILKHISVSSVSASPTALEDAIADIARK
                     YNASSWKVTSMRIDNNSTATAVLYK"
BASE COUNT          271 a          209 c          200 g          265 t
ORIGIN      
        1 aaaacctttt aacagggagt gatattgatc ttcactctgt catatctccg gtaatatggc
       61 gtcaggcttc atcatcagaa agggggcctg acgcctgaaa aagtgaacaa cagacagtgt
      121 tcggattatc aatattcagt ttttaacgtt gaactggatt cattactgcg ttgtttaaga
      181 atttcccgcg ccgaatctgc cattatcatg actgcacctg ccagcatcag agtatctttc
      241 aataccaggc gaccagcacc ggataaataa gggaaaccat gatgagcgtc acccaatgcg
      301 ggtacccatg cctccggggt ggtgattaaa aatgagagtg ttaccaacgg tgtggtaaat
      361 gccatcagcc ctcccaataa acctaaccag cgattgacag gattagccaa aaccagcaga
      421 gcaataatca cctccacgac gccaagaccg ttggaaaaac cataggtatt attggccgat
      481 tgccatgccc ttgcttctgg tttgtattcg ccttcgtgag tcagatactg tttatagtct
      541 tccgggtgtt cataaaagaa cgacattagt ggactgtttg cgacgaatgg tgtaatgctg
      601 tctgcctcgt aagggacaaa ctttaataac ccaatccaca taaaaacaat tgcaatactc
      661 agacgaatca atgtcaggcc aattttatct ccccgactta acaggtgcag gtatttttcc
      721 attgtgaaca tccttattta tacaatactg ctgttgcggt tgaattatta tcaattcgca
      781 tcgatgtgac tttccaggat gaagcattat attttctggc tatatcagca atggcatcct
      841 ccagagctgt cggtgatgct gatactgacg agacgctgat atgtttcaga attattttat
      901 catctgcatt ggtggaaaat gccataacgc cagataatag tgttg
//
LOCUS       DAPHBJ010000404          945 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.407, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000404 DAPHBJ010000000
VERSION     DAPHBJ010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.407"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(82..>945)
                     /locus_tag="RHZ76_004618"
     CDS             complement(82..>945)
                     /locus_tag="RHZ76_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HDV2815098.1"
                     /translation="KDVVEINPYAIMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLRE
                     GKALYDNLHPSRIVIGERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTY
                     LAMRVAYFNELDSYAESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQL
                     LANYQSVPNNLISAIVDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQ
                     GIMKRIKAKGVEVIIYEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVAD
                     KVYTRDLFGSD"
BASE COUNT          269 a          210 c          213 g          253 t
ORIGIN      
        1 ataggataaa tgataaaaac aatgagttat tgcacgggtt acaaaagaca atcagggcga
       61 aaccgccctg atcaaagaag attagtcgct gccaaagaga tctcgtgtgt agactttgtc
      121 tgccacatcc ttaagttctt ctgccatacg gttggagata ataacgtcgg cttgttgttt
      181 gaaggtcgcg agatcacgtt ccaggcgaga gttgaagaat gagtcttctt tcatcaccgg
      241 ctcgtaaatg ataacttcaa cgcctttcgc cttgatacgt ttcataatcc cctgaatgga
      301 agacgcacgg aagttatctg aaccgctctt cataatcaga cgataaatac ccaccacttg
      361 cggcttgcgt gacaaaatgg catcggcaat aaaatcttta cgcgtgcggt ttgcatcgac
      421 aattgccgag atcaggttat tcggcacaga ctggtagttc gccagtaact gcttggtatc
      481 tttcggcaga caataaccac cataaccaaa cgacggattg ttgtaatggt tgccaatacg
      541 cggatcgaga caaacgcctt cgataatctg gcgagtattc agacctaaac tttctgcata
      601 gctatccagc tcattaaagt acgctacgcg catcgccaga taggtattag caaaaagttt
      661 aatcgcttct gcttcagtgg agtcggtaaa cagggtcggg atattttgct taatcgcgcc
      721 ttcctgtaac aacgcagcaa aacgttctgc gcgttctgaa cgctcaccaa tgacaatacg
      781 tgacggatga aggttatcgt aaagggcttt accttcgcgg aggaattcgg gtgagaatat
      841 aatattttca gtgcgatatt tcttatgcat cgctgcggta aaaccaacgg gaaccgttga
      901 tttgatgacc ataatcgcat aaggatttat ctcaactacg tcttt
//
LOCUS       DAPHBJ010000405          940 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.408, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000405 DAPHBJ010000000
VERSION     DAPHBJ010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.408"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            72..647
                     /locus_tag="RHZ76_004619"
     CDS             72..647
                     /locus_tag="RHZ76_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HDV2815099.1"
                     /translation="MNDNYPYIAKYKMDWFLHSLINHHLNSVIERYSKVLGIRLDFSY
                     NQNSERYKKDYSCLQQDLRKLFKQVEQEHGIAGFFWVIEYGEDNRFHSHTVVYLDGQK
                     IRKTFPVAERIGNLWREITQGDGKYHRCEHKENYKADINKIINHRDTDYIQGLRYIIS
                     YLAKQEQKYDMNIYGSSEVPPPSGLGRPRNL"
     gene            789..>940
                     /locus_tag="RHZ76_004620"
     CDS             789..>940
                     /locus_tag="RHZ76_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HDV2815100.1"
                     /translation="MYTKPALLEDQFIDMKFITQLTGLSDKWFYKLIQDGLFPKPIKL
                     GRTSRW"
BASE COUNT          319 a          182 c          190 g          249 t
ORIGIN      
        1 gccatcaaac cacctgacaa aaaaatacaa cacatccctt tcctctggac cataacctca
       61 gaggaaataa catgaacgat aactatccat acattgccaa atataaaatg gactggtttt
      121 tacatagcct gataaatcac cacctgaata gtgtcattga acgctattca aaggtgctcg
      181 gtatccgact ggacttttcg tacaaccaga actcagaacg ttacaaaaag gactacagtt
      241 gccttcagca ggatcttcgg aaactattta agcaagtcga acaggaacat ggtatagctg
      301 gatttttctg ggtaattgaa tatggtgagg acaataggtt tcattctcat acagtggtgt
      361 atctggacgg acaaaaaata cgtaaaacat ttccggtagc agaacgtatt ggtaatttat
      421 ggagggagat aactcaggga gacggtaagt atcatcgatg tgaacacaag gagaactata
      481 aagctgatat aaataagatc attaaccatc gggatactga ctatatccaa ggattaagat
      541 atatcatcag ctatcttgct aagcaggaac agaagtatga catgaatatc tacgggagta
      601 gtgaagtacc accaccaagt ggattaggcc gcccaaggaa cttataagcg aagtatttat
      661 gtaggtaagg tttacccttg cctatgttcc aaactttttt acaacagaag ccattgatgt
      721 ggaggagctg gcagtaaata attttactaa cggctccggt tatttatcct taaccggaac
      781 acatcaccat gtatacaaaa cctgctctac ttgaagatca attcattgat atgaaattca
      841 ttacccagct tactggcctg agcgataaat ggttctataa gctgatacag gatggattat
      901 tcccgaagcc tattaagttg ggaagaactt cacgatggtt
//
LOCUS       DAPHBJ010000406          939 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.409, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000406 DAPHBJ010000000
VERSION     DAPHBJ010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.409"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..306)
                     /gene="hdeD-GI"
                     /locus_tag="RHZ76_004621"
     CDS             complement(<1..306)
                     /gene="hdeD-GI"
                     /locus_tag="RHZ76_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat resistance membrane protein HdeD-GI"
                     /protein_id="HDV2815101.1"
                     /translation="MNTDTITDSSADDPTKALCSLSQNWWLFVLRGVLALIFAALAFW
                     MPQSALLAMTIMFGAFSLVNGAFNLFAAVRHIQKKERWGWLLFSGIVGILTGVVVLVA
                     "
     gene            complement(396..>939)
                     /gene="yfdX2"
                     /locus_tag="RHZ76_004622"
     CDS             complement(396..>939)
                     /gene="yfdX2"
                     /locus_tag="RHZ76_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heat resistance protein YfdX2"
                     /protein_id="HDV2815102.1"
                     /translation="HDLFANTETIEAMTDEALDALKHGEVQQARHVLALLASEIVITV
                     TNIPLASYPAAVKAVVPLIDQGKIEEAKAALQSALSTLVEERSVLPLPVLRAKLLLKR
                     AEPLVEDGQRSEASNERLETLLNEARQQLEMAELLGYGKRKDFEPLYAELKKIKEKTG
                     GGGFGKGWLDEVKAKLSRLF"
BASE COUNT          212 a          278 c          243 g          206 t
ORIGIN      
        1 agcaaccagg acgacgacgc ccgtaagtat gccgacaatg ccgctgaaca acaaccagcc
       61 ccagcgctct tttttctgga tatggcgcac cgctgcaaac aagttgaacg cgccattgac
      121 caaggaaaat gcgccgaaca tgatggtcat ggccaacaga gccgattgtg gcatccagaa
      181 cgcgagggct gcaaaaatca atgccaagac accgcgcagc acaaaaagcc accaattttg
      241 gctaagcgaa caaagtgctt tggtggggtc atccgcactg gaatcggtga tggtgtctgt
      301 gttcatgcaa tatctcctaa agatctatac aaaataaaaa atcaaacact catgggacaa
      361 gagagggggc actatgcgcc ccctctccag cggtttcaga acaacctgga cagttttgcc
      421 ttgacttcat cgagccagcc ttttccgaag cctccccctc ccgtcttttc cttgattttc
      481 ttgagttcag cgtacagggg ctcaaagtcc ttcctttttc catagcccag cagttctgcc
      541 atttccaact gctgccgggc ttcgttcaac aatgtctcca ggcgctcatt ggatgcttcg
      601 ctgcgctgac catcttctac cagaggctcg gcgcgcttca ggagcagctt cgcacgcagg
      661 acgggcagcg gaagcacgct gcgctcctcg accagcgtgc tgagcgctga ctgcagcgcg
      721 gctttggctt cttcgatctt gccctgatcg atcagcggca cgactgcctt cacggctgcg
      781 ggataggacg ccagagggat gttggtgacc gtgatcacga tttcactggc cagcaaagcc
      841 agcacgtggc gagcctgttg cacctcgcca tgtttgaggg catccagcgc ctcgtcggtc
      901 atcgcctcaa tggtttccgt gttggcgaac aagtcgtgc
//
LOCUS       DAPHBJ010000407          935 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.410, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000407 DAPHBJ010000000
VERSION     DAPHBJ010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.410"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>935
                     /locus_tag="RHZ76_004623"
     CDS             <1..>935
                     /locus_tag="RHZ76_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HDV2815103.1"
                     /translation="SALWRNYWGRSGNAKDYQFSYSNNWQHISYTFSASQSYDENNKE
                     EERFNLFISIPFYWGDDIAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYG
                     IYVNQQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWSGGINIT
                     NQLSDTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDPIIPHRENHLVLDIANSESET
                     ELQGNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVV
                     GQGSRLFIRVDEIPTGIKVALNDEQNLFCTITFQH"
BASE COUNT          289 a          189 c          192 g          265 t
ORIGIN      
        1 taagtgcgct ttggcggaat tactgggggc gaagtggaaa tgctaaagat taccaattca
       61 gttactccaa taactggcaa cacattagtt atactttctc tgccagccaa tcttatgatg
      121 aaaataataa agaagaggag cgttttaatc tgtttatctc cattcctttc tactgggggg
      181 atgatattgc caaaacacgt caccaaatta acttatcgaa ttcgacctca ttttccaaag
      241 atggctattc ctccaacaat actggaatta ctggcatagc cggtgaacat gatcagttaa
      301 attatggtat atatgttaat cagcaacaac aaaataatga tacctcgctt ggtacgaatt
      361 taagctggag aactcccatc gccataatag atggcagcta tagtcattct aaaaacgcct
      421 ggcaaagtgg tggaagtatt agtagtggat tagttgtctg gtccggtggt attaatatca
      481 ctaaccagtt atccgataca tttgcaattc tggatgcgcc tggattagaa ggcgcgcata
      541 ttaatggaca aaaatacaac cgaacaaaca gcaaaggcca ggttgtttac gacccgatta
      601 tacctcatcg tgaaaaccat ctggtacttg atatagcaaa cagtgaaagt gaaacagaat
      661 tgcagggcaa tcgtcaaatt attgcgcctt accgtggagc agtttcttat gtgcagttta
      721 caactgacca acgtaagccc tggtatatac aggcactgcg tcccgatggt tcgccattaa
      781 cctttggcta tgacgtactg gatctccagg aaaacaatat tggagtcgtt ggccagggta
      841 gtcgcctttt tattcgcgta gatgaaattc caactggcat aaaagttgct ctcaatgatg
      901 aacagaattt attctgtact attacttttc aacac
//
LOCUS       DAPHBJ010000408          934 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.411, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000408 DAPHBJ010000000
VERSION     DAPHBJ010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.411"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            158..877
                     /locus_tag="RHZ76_004624"
     CDS             158..877
                     /locus_tag="RHZ76_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDV2815104.1"
                     /translation="MDKLLNKKIKVKQSNELTEAAYYLSLKAKRVLWLCLMQTYFTAS
                     VSEDDDEMAVLGDSTFKVKVADYQQIFQVSRNQAIKDVKEGVFELSRSAVIFYPKEGS
                     FDCVARPWLTEAGSRSARGIWEIEFNHKLLRYIYGLTNQFTTYSLRDCGSLRNPRTIR
                     LYESLAQFKSSGLWVTTHAWLNDRFLLPESQQKNLAELKRSFLDPALKQINEKTPLLA
                     KYSIDDSGKFLFSIIDKQNPV"
BASE COUNT          267 a          203 c          203 g          261 t
ORIGIN      
        1 ccacctatct atgggaaaca ccccacctta ctatgggaat tagcccacct tgttatggga
       61 attggcccac cttagacgaa actgtaaaaa atgtatttac ttgtttgaac tttgtggtag
      121 tgtggagagt aatttttaac ccacaaaggc aaggctcatg gataagttgc tgaacaaaaa
      181 gataaaagtt aagcagtcta acgagcttac cgaagctgct tactacctct cgctaaaagc
      241 aaagcgcgtt ctctggttat gtcttatgca gacgtatttc acagcttcag taagcgaaga
      301 tgatgatgag atggctgtac tcggtgactc tactttcaaa gtaaaggtgg ctgactatca
      361 gcaaattttt caggtaagcc gtaaccaggc tatcaaggat gttaaagaag gcgtgtttga
      421 gttaagccgt tctgcggtaa tcttttaccc gaaagaaggg agttttgact gcgtcgcgcg
      481 cccctggcta acagaggctg gcagccgatc agctcgtggt atctgggaaa tcgaatttaa
      541 ccataaactc ctgcggtaca tttacggcct gacgaaccag ttcaccacct actcgctccg
      601 cgattgtggc agtcttcgaa atccacggac gatccgcctt tatgaaagtc ttgctcaatt
      661 caaatcttca ggcttatggg ttactactca tgcttggtta aatgaccgtt tccttttgcc
      721 ggaatcccaa cagaagaact tggcagagtt gaaacgatct ttccttgatc ctgctctcaa
      781 gcagataaat gagaaaacac ctttacttgc taagtatagt attgatgatt caggaaaatt
      841 tctgttctca ataattgata agcaaaatcc cgtctgacat aaatcagcac acatgagcct
      901 gtcatttgac aaatttttgt catgaagatg ggcg
//
LOCUS       DAPHBJ010000409          931 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.412, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000409 DAPHBJ010000000
VERSION     DAPHBJ010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.412"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..820
                     /gene="yeeO"
                     /locus_tag="RHZ76_004625"
     CDS             <1..820
                     /gene="yeeO"
                     /locus_tag="RHZ76_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HDV2815105.1"
                     /translation="VGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFS
                     IIWEVMGIGIPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALTNLPGSAL
                     GSASTIITGRRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQ
                     VKHVVVILIWLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLG
                     IMLGWGVVGVWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
BASE COUNT          188 a          183 c          268 g          292 t
ORIGIN      
        1 tgtcggggct gggttggggt taaccatttc tcgttatatt ggcgcagtgg caattttgtg
       61 ggtgctggcg attggtttta atcctgcgct aaggatttcg ttaaagagct attttaaacc
      121 gctgaatttt agcattatct gggaagtcat ggggattggt attcccgcga gtgtcgaatc
      181 agtgttattt accagtggtc ggttattaac ccaaatgttc gttgccggga tggggaccag
      241 tgttattgcc ggaaatttta tcgcgttttc aattgcggct cttaccaact tacccggaag
      301 tgcgctcggc tctgcttcta cgatcattac aggccgaagg ttgggtgtag gacagatagc
      361 gcaagcagag attcagttgc ggcatgtgtt ctggctttcc actcttggat taacggccat
      421 cgcctggcta acggctccct ttgccggggt tatggcatcg ttttacaccc aggatccaca
      481 ggttaaacat gtcgttgtga ttctgatttg gctaaatgct ttatttatgc ctatttggtc
      541 tgcctcatgg gtgctacccg ctggatttaa gggtgctcgc gatgcacgtt acgccatgtg
      601 ggtttctatg ttgagcatgt ggggttgccg ggttgtagtc ggttatgtgc tgggaatcat
      661 gcttggctgg ggtgtggttg gtgtctggat gggaatgttt gccgactggg ctgtgcgggc
      721 cgtgctgttt tactggcgaa tggttactgg acgttggctg tggaaatatc ctcgacctga
      781 gccgcaaaag tgtgaaaaaa agccagttgt gtcggaataa acgacaaagt gcagattatt
      841 tcagcaaacg atttcaaatt taaaaaacag gctttgacat tgtggatgag cgtcgctaat
      901 attcgcctcg ttctcacgat tcctctgtag t
//
LOCUS       DAPHBJ010000410          926 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.413, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000410 DAPHBJ010000000
VERSION     DAPHBJ010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.413"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..350)
                     /locus_tag="RHZ76_004626"
     CDS             complement(<1..350)
                     /locus_tag="RHZ76_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HDV2815106.1"
                     /translation="MNNTLLPSINIPCTLFETISLFDDYSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMFS
                     FFGQYKTLIEDLIE"
     gene            503..679
                     /locus_tag="RHZ76_004627"
     CDS             503..679
                     /locus_tag="RHZ76_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HDV2815107.1"
                     /translation="MLNLPIRKLFPTRIASSYGMNFEFMPELKWGFGYPGAIIFMILA
                     GLAPYLYFKRKNWL"
     gene            complement(720..>926)
                     /locus_tag="RHZ76_004628"
     CDS             complement(720..>926)
                     /locus_tag="RHZ76_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815108.1"
                     /translation="FFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
BASE COUNT          299 a          168 c          171 g          288 t
ORIGIN      
        1 tgttcaatca gatcttcgat aagtgtttta tattgtccaa aaaaggaaaa catttttgct
       61 agttctttca tttctgcaaa taaaatatca gtgcattcac gttgtgatat ttttgttccg
      121 gaacttgagg cactaaaagc aacattaata gaacctgggc cagggaaatg gtatttaata
      181 agtctgtaag gatcaacttt tgctgagata tcgcttaggc cgagtgagag aaaatcttgt
      241 tctaccatat caccatattg catatcatca gcagaataat catcaaataa actaatagtt
      301 tcgaaaagtg tacagggaat attaattgat ggaagtaatg tgttattcat ttacattttc
      361 cttagttata tttaagctca atatcaattc gagaacaaat gctagggtaa aaaaatttta
      421 aaaaagaagt acacttcaca tttttattac attctgaagg gaatctcgtt attgattggt
      481 tttgcccatt gttaataaca aaatgttgaa tttaccgatt aggaaacttt ttcctacgcg
      541 gattgcctcc agctatggca tgaactttga gtttatgcca gaactgaagt ggggcttcgg
      601 ctaccctggc gcgattatct ttatgatcct cgcgggcctg gcaccgtatc tgtactttaa
      661 gcgcaagaac tggctgtaaa aagaaggcgg tggcttagct tggctaagcc acctgttatt
      721 caaaggctcc aggtatttaa cccttttacc tctttctcat aaaaccattt gttcgtgtta
      781 acagcaacat aggctgctac ggcaatgccc agaatgttaa cgccaattag tgcaccaaca
      841 aatagagata gaataccaag taatagaact ataattgctt ttttccataa ccccaggaca
      901 aataaatata tccaggaaca gaagaa
//
LOCUS       DAPHBJ010000411          919 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.414, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000411 DAPHBJ010000000
VERSION     DAPHBJ010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.414"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(106..>919)
                     /locus_tag="RHZ76_004629"
     CDS             complement(106..>919)
                     /locus_tag="RHZ76_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDV2815109.1"
                     /translation="LYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYY
                     QSYLANADIEIVPNGIDLETYQSTFQPITRSELNISPEEKIIFYAGRIVPDKGILLLM
                     QSFEKLAAAHKNLKLVVIGDYTEMSKSDKGAYQHNVREIAKRLKDRCIMLGSIPPEKM
                     HCYYPLADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEP
                     MTADSISSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
BASE COUNT          280 a          189 c          154 g          296 t
ORIGIN      
        1 cgtagaacaa attgaaccaa atttattagg cgtttacgcc ggcttattat tagcaatgaa
       61 aaacaagtaa aaaaataaaa aggctgcata atgcggcctt tttatttatt caaaccaatt
      121 atgaatgacc tcttcgaatc tttgagttac gccatcccaa ctgtagtgat caaacacaaa
      181 atcctgacct tgctttgcaa ctgctgttaa ttcaggattc gctaacgttt ttaaaatatc
      241 gctgcttatg gagtctgcag tcataggttc ttttaaatgg aatcccgtag tattttcttt
      301 gacaaattcc gtcatccccc cacgcgtact cactaaaaca ggcttaccag cccccatagc
      361 ttcaattgcc accatgcaaa atggttcctg gaactgggaa ggaatgacaa ctaaatctgc
      421 cagtgggtaa taacaatgca tcttttcagg cggtatgctt cctaacataa tgcaacgatc
      481 cttcaaacgt tttgcaattt ccctgacatt gtgttgatat gcacctttgt cacttttact
      541 catttctgta taatcaccaa tcacaactaa ttttaaattt ttgtgagcag cggccaattt
      601 ttcgaatgat tgcatcaaca gtaatatgcc tttgtcaggg acgatacgcc cagcataaaa
      661 aataattttc tcttcagggg agatattaag ttcgcttcta gtgatcggct gaaaagtaga
      721 ttgataggtt tctaaatcaa ttccatttgg tacaatctca atatctgcat ttgctaaata
      781 agattggtaa tactttttca agtataaact aggcactatc attttaacat tttgctcaag
      841 caattttggt tcaaatgcat tatgcatatg aataaccact cttgcctgag gagctcgctt
      901 acgaatttga gtgtatagc
//
LOCUS       DAPHBJ010000412          887 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.415, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000412 DAPHBJ010000000
VERSION     DAPHBJ010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.415"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(22..>887)
                     /gene="yfdE"
                     /locus_tag="RHZ76_004630"
     CDS             complement(22..>887)
                     /gene="yfdE"
                     /locus_tag="RHZ76_004630"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HDV2815110.1"
                     /translation="DVLAENFRPGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLK
                     DAPAYDTIIQAMSGIMMETGYPDAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQR
                     GAHVDIAMFDATLSFLEHGLMAYIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCG
                     NDKLFSALCQALELTELVNDPRFSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHE
                     VGVPVAPLLSVAEAIKLPQTQARNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQ
                     HGEQIRQEFSS"
BASE COUNT          227 a          231 c          190 g          239 t
ORIGIN      
        1 atttaccagc acatcttaca tttatgatga gaactcctgg cgaatttgtt ccccatgctg
       61 gtcgagcgtt gccgctcccg gcataacatg cgggtccgcg cagccgctga tttttatcgg
      121 attacccggc atcattattc ccccggcttc aatcaacata tttctcgcct gagtttgtgg
      181 caatttaatg gcctcagcca cacttaataa cggcgcgacg ggtacaccaa cttcatgtat
      241 tctggctaac caaacttcag ctgcctgcgt ttttaacgtc cgctcaatat attgtttaag
      301 aatagcctgg ttttgtacgc gtaaaatatt gctgctaaat cggggatcat taaccagttc
      361 cgtaagctcc agtgcctggc ataacgcaga aaaaagcttg tcattaccac aacaaatcgt
      421 aatcggctta tcctgagtat tgaaaacatc aaaaggtgcc atgtagggat ggcgatttcc
      481 cagacgttgt ggtgacttcc cagttgcgat atatgccatc agaccatgct ccagaaaact
      541 cagcgtggca tcaaacatcg ctatatcgac atgcgcccct ctctggctct tttcgcggcc
      601 ataaagtgca ctcactattc cgctgaataa atagacaccg ccgcatagat ccgcaagaga
      661 tgtaccaacg cgcactggcg gagcatcagg atatcctgtt tccatcatta tcccgctcat
      721 tgcctgaatg atggtatcgt aggcaggagc atcttttagc ggaccggtat gtccgaaacc
      781 tgacgatgaa gcatatatga ggcgcgggtt gatttcttga agcgtttccc atgaaaaccc
      841 cagtttttcc attgtacctg ggcgaaaatt ctcagctaat acatcag
//
LOCUS       DAPHBJ010000413          885 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.416, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000413 DAPHBJ010000000
VERSION     DAPHBJ010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.416"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>885
                     /locus_tag="RHZ76_004631"
     CDS             <1..>885
                     /locus_tag="RHZ76_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815111.1"
                     /translation="NNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPLIDELR
                     HQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNAGKLMTEFDDN
                     GKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLELAIGW
                     RGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGGVATAI
                     GSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDGLKRQQ
                     ELDKWKRENMTPFMYVLN"
BASE COUNT          278 a          161 c          180 g          266 t
ORIGIN      
        1 ggaataacaa caacacacag aatgcacagt gggctgatta tgtgggtgac attcttcggg
       61 gggctcagcc gataaaccag ctagtacctc agcaccctta tcttaacgac gtcccactca
      121 ttgatgagtt aaggcatcag aacactcatc atgttattct cttgacactt gatgttgcaa
      181 aaaaaatact cagcccaata acatcatttg attacattca ttttatcact acacatcctt
      241 caggtataaa agatacactc gcctggttag ttaacgcggg taaattaatg actgagtttg
      301 atgacaatgg gaaaattata tttaacctta atgccttaaa gtatacaaaa gcaagttact
      361 ttgaaatact tggagagaaa tatataaaaa tcacaacatc cagcccctgg cttttagaaa
      421 agctcggtaa atatattttc tcttcacgag cgcctcaagt tctggaactc gctattggct
      481 ggcgaggagc tttatctgaa tcaataaaag gagttaagtt ctgtatctgg ttttcagtag
      541 cctggagaac catagaattt attatgagtt cagagcgaga tctggttaat tttcttggcg
      601 atttttcaat ggatgtggcc aaagcagtta tagccggtgg tgttgcaacc gctattggaa
      661 gtctggcttc ttttgcctgt gttagctttg gctttccagt aattcttgtc ggaggagcaa
      721 ttttactgac agggatagtg tgtacagttg ttttaaatga aatcgatgct caatgccatt
      781 tatcagaaaa attaaaatat gcaattagag atggactaaa acggcaacag gaacttgata
      841 aatggaaaag ggaaaacatg actccattta tgtatgttct taaca
//
LOCUS       DAPHBJ010000414          882 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.417, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000414 DAPHBJ010000000
VERSION     DAPHBJ010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.417"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..483)
                     /gene="sctT"
                     /locus_tag="RHZ76_004632"
     CDS             complement(<1..483)
                     /gene="sctT"
                     /locus_tag="RHZ76_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HDV2815112.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELSTLNEIDIALFAAREIIIGLFIACLLSSPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTNGGLNFILETLWQSYNLWPSG
                     N"
     gene            complement(485..745)
                     /locus_tag="RHZ76_004633"
     CDS             complement(485..745)
                     /locus_tag="RHZ76_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HDV2815113.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(755..>882)
                     /locus_tag="RHZ76_004634"
     CDS             complement(755..>882)
                     /locus_tag="RHZ76_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HDV2815114.1"
                     /translation="MMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLSINP"
BASE COUNT          307 a          192 c          151 g          232 t
ORIGIN      
        1 attgcctgaa ggccataaat tataactttg ccaaagagtt tccagaataa agttcaatcc
       61 accgttagta agataaaccg ctgcagagaa taggttaaat aatcttgcta attccgaagt
      121 atcgacacct gtagcaggat ccaaagtact acttaacgtt gcaccacgtt ggttatcgat
      181 aaaactacct atagcaagga aaatccaaaa cggggatgat aataagcaag caatgaacag
      241 ccctatgata atttctctgg cagcaaacaa agcgatatcg atttcattta aggtcgataa
      301 ttcatagtgg taatgctgaa ctaatgcaca tgataccact attattattg gatgacggac
      361 tactgttggt atattcccac tggcgaaaaa tggtaacaaa tagaaagttg gtgccaatcg
      421 gcaaaaaccg agcgctgtag cagccagcag actatgcaac tgatagagta tcgcctcccc
      481 catcctatcc tctcgcgaat gctaaattca gcatttcgat accaaacgaa tataattttt
      541 caccatacca tccgctcatt aaaaaaaagc aaatacttac gcataataat ttgacgccaa
      601 atggcaacgt ctgttcttgt aactgtgtta ccgtttgaaa aagccctacc aataaaccaa
      661 caaatgtcgc aactgcaata ggtcctgcag acataactaa aatcagatac agcgccctat
      721 tccccgcaaa aacaatatcg tccatatttt tttctcaggg attaatggat aggtcaaaat
      781 attgtaaaat gagtcctttt gataacattg tccaaccgtc catcgcgaca aacaaaatta
      841 attttatcgg tgtagatatg gttaccggac tcatcatcat ca
//
LOCUS       DAPHBJ010000415          882 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.418, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000415 DAPHBJ010000000
VERSION     DAPHBJ010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.418"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(108..392)
                     /locus_tag="RHZ76_004635"
     CDS             complement(108..392)
                     /locus_tag="RHZ76_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HDV2815115.1"
                     /translation="MYHYLVFVPLALIISAAALALPWFIVRAGYRDLVQITCACVSIL
                     GLCAATGVFSYFDNKPLAFLYGCGALACFLYAVDCAIPLYMTRKKPRKQL"
     gene            complement(525..821)
                     /locus_tag="RHZ76_004636"
     CDS             complement(525..821)
                     /locus_tag="RHZ76_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HDV2815116.1"
                     /translation="MHFTNFLQRYFDIEIEHTFDPTIQGSNETGKDVTKIWIYEKGED
                     SEPLLTLTEAWWYTETKTAGNWLIGNVYSTLEHGREIHESEFRKLVTAGKVISA"
BASE COUNT          219 a          236 c          216 g          211 t
ORIGIN      
        1 gacaccgcac cgtcacggcc agcgccccgc tgagatgcac aatcgagtgc ctgaatcgct
       61 tcctgacagc ctcaataaaa aaaggcgggg attacccgcc ttttttctta cagctgctta
      121 cgtggctttt tacgcgtcat atacaacggt atcgcgcagt ctaccgcgta caaaaagcac
      181 gccagcgcgc cgcaaccgta cagaaacgca agcggcttat tatcgaagta gctgaaaacc
      241 cctgtcgccg cacacaggcc cagaatagag acgcaggcgc aggtgatctg caccagatcc
      301 ctgtatcccg cacgaacgat aaaccagggc agggcaagcg cagcggcgct aataattaat
      361 gcgagaggga caaaaacgag atagtgatac atgtgaactc cttaatggtt gccgataccg
      421 gcgattgttc gggcggcggt attgccaccc gatgatttaa ttagaggttt tgcgcgtcca
      481 ggagattgac ctgagccggg gtaacgtgaa acttttcccc ttactcatgc tgatatcacc
      541 ttgccagctg ttaccagttt acgaaattcg ctttcatgaa tttcacgccc atgctcaagc
      601 gttgaataca cgttgccgat aagccagtta ccagccgttt ttgtctcggt ataccaccat
      661 gcttctgtca gagtaagcag cggttcgcta tcctcgccct tctcatagat ccagatttta
      721 gtgacgtctt tacccgtttc gttgctgccc tgaatggtag ggtcaaaggt atgttcaatc
      781 tctatatcga agtaacgctg aaggaagtta gtaaagtgca tgacgactcc tgtaagcgcc
      841 gataccggca atttttcggg tggcggtatt gcctcccgat ga
//
LOCUS       DAPHBJ010000416          880 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.419, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000416 DAPHBJ010000000
VERSION     DAPHBJ010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.419"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..517)
                     /locus_tag="RHZ76_004637"
     CDS             complement(<1..517)
                     /locus_tag="RHZ76_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815117.1"
                     /translation="MQIQYSYRAIDKEFHEPWQRALGNVIEHALKPLLDKHTKDSARL
                     QIVLDRDKIKRQFLASGYMHLPGKKIVSVTASHDELTPLAQMLAKSLFRQAKKHFDRL
                     HAQDQIKRKARRERLRELKVRIAAKPASAAHEAEVIRMPLLSKLEAVARRELAYLRAV
                     GDLPRDYPTLRD"
     gene            complement(597..>880)
                     /gene="hdeD-GI"
                     /locus_tag="RHZ76_004638"
     CDS             complement(597..>880)
                     /gene="hdeD-GI"
                     /locus_tag="RHZ76_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heat resistance membrane protein HdeD-GI"
                     /protein_id="HDV2815118.1"
                     /translation="ASFLWASVGFWAIFTGVLEISAAVRLRREIKGEIWLALSGLLSI
                     ILGAIVLWIFFTRPVESFVAAGWLLGFHAAVYGVTLLFLSWSLRKTRQG"
BASE COUNT          212 a          241 c          230 g          197 t
ORIGIN      
        1 cgtcgcgcag cgtcggataa tcgcgcggta aatcgccgac ggcccgcaga taagccagct
       61 cacgccttgc gacagcctca agtttcgaca gtagaggcat tcggatcacc tcggcctcat
      121 gggcggctga tgcgggtttt gcggcaatcc gcaccttgag ctcgcgcaag cgctcgcgtc
      181 gtgctttgcg cttgatttga tcctgagcat gcagtcggtc aaaatgcttc ttggcctgac
      241 ggaacaacga ctttgcgagc atctgcgcga ggggcgtcag ctcgtcatgt gatgcagtaa
      301 cactgacaat ctttttgccg ggcaggtgca tatagccact ggccagaaat tgccgcttga
      361 tcttgtcgcg atcaaggacg atttgaagtc gcgctgaatc tttagtgtgt ttgtcgagca
      421 atggcttgag ggcgtgctcg atcacatttc ccaaagctct ctgccaaggt tcatgaaact
      481 ctttgtcgat agctcgatag ctatattgta tctgcatgta tgactccttc gatgtcattt
      541 ctatggaggc atgaatcgag gatgctcata tccatagatt ttggtttggc acgctcttac
      601 ccctggcgcg ttttgcgaag actccaactc aagaacaaaa gcgtgacgcc atagactgcc
      661 gcatggaaac ccaacagcca gcctgcggcc acgaatgact cgaccgggcg ggtaaaaaat
      721 atccacaaga cgatagcacc aaggataatc gagagcagtc cactgagggc aagccagatt
      781 tcacccttga tttcccgacg cagccgaacg gcggcagata tctccagcac accggtgaaa
      841 atcgcccaga agcccacgct agcccataaa aaagaagcca
//
LOCUS       DAPHBJ010000417          873 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.420, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000417 DAPHBJ010000000
VERSION     DAPHBJ010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.420"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            14..799
                     /locus_tag="RHZ76_004639"
     CDS             14..799
                     /locus_tag="RHZ76_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815119.1"
                     /translation="MNLRRVCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YQDMLQTGKYDSALKSTVWSDYKNDKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          282 a          175 c          191 g          225 t
ORIGIN      
        1 ggagtgtccg attatgaatt taagaagggt ttgttgtata tgtgtattat tctcgctgct
       61 tgcaggatgt gcctctgaat cttctattga tgaaaagaag aaaaaagctc aagtcacaca
      121 aagtaatatt aataaaaaca ctccccagca actgacagac aaagatttat tcggtaatga
      181 aaccactctg gccgtatccg aagaggatat tcaagctgcg cttgatggag atgagttccg
      241 cgttcccctt aattctcctg taatacttgt tcaatccggc aaccgcgcac cggaaaccat
      301 tatgcaggag gagatgcgaa aatattatac tgtttccaca ttctctggta tcccggacag
      361 gcaaaagcct ctgacttgta acaaaaacaa agataaaaat gagaacgagg atgttgccag
      421 tgctgagaat atgaactgga tgcaggcact gcgttttgtg gctgcaaaag gacatcagaa
      481 agcgattatt gtttaccagg atatgttgca gacaggaaaa tatgactctg cgctgaaatc
      541 aacagtatgg tccgactata aaaatgacaa actcactgac gctatctccc tgcgctacct
      601 ggtacgtttc acgctggtgg atgtggcaac aggtgagtgg gctacctggt cgccggtgaa
      661 ttatgaatat aaagtgcttc caccattgcc cgacaagaat gaagccagta cgactgatat
      721 gacagagcag caaatcatgc aacttaagca aaagacctat aaagcgatgg taaaagattt
      781 ggttaaccgc tatcaataat aaattatatc tgccgccagg aatttctggc ggcaataata
      841 caaaattctt tgcataagaa atttttcttg aca
//
LOCUS       DAPHBJ010000418          862 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.421, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000418 DAPHBJ010000000
VERSION     DAPHBJ010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.421"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            32..>862
                     /locus_tag="RHZ76_004640"
     CDS             32..>862
                     /locus_tag="RHZ76_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS186B family transposase"
                     /protein_id="HDV2815120.1"
                     /translation="MNYSHDNWSAILAHIGKPEELDTSARNAGALTRRREIRDAATLL
                     RLGLAYGPGGMSLREVTAWAQLHDVATLSDVALLKRLRNAADWFGILAAQTLAVRAAV
                     TGCTSGKRLRLVDGTAISAPGGGSAEWRLHMGYDPHTCQFTDFELTDSRDAERLDRFA
                     QTADEIRIADRGFGSRPECIRSLAFGEADYIVRVHWRGLRWLTAEGMRFDMMGFLRGL
                     DCGKNGETTVMIGNSGNKKAGAPFPARLIAVSLPPEKALISKTRLLSENRRKGRVVQA
                     E"
BASE COUNT          204 a          214 c          243 g          201 t
ORIGIN      
        1 ggtattacgc ctgatatgat ttaacgtgcc gatgaattac tctcacgata actggtcagc
       61 aattctggcc catattggta agcccgaaga actggatact tcggcacgta atgccggggc
      121 tctaacccgc cgccgcgaaa ttcgtgatgc tgcaactctg ctacgtctgg ggctggctta
      181 cggccccggg gggatgtcat tacgtgaagt cactgcatgg gctcagctcc atgacgttgc
      241 aacattatct gacgtggctc tcctgaagcg gctgcggaat gccgccgact ggtttggcat
      301 acttgccgca caaacacttg ctgtacgcgc cgcagttacg ggttgtacaa gcggaaagag
      361 attgcgtctt gtcgatggaa cagcaatcag tgcgcccggg ggcggcagcg ctgaatggcg
      421 actacatatg ggatatgatc ctcatacctg tcagttcact gattttgagc taaccgacag
      481 cagagacgct gaacggctgg accgatttgc gcaaacggca gacgagatac gcattgctga
      541 ccggggattc ggttcgcgtc ccgaatgtat ccgctcactt gcttttggag aagctgatta
      601 tatcgtccgg gttcactggc gaggattgcg ctggttaact gcagaaggaa tgcgctttga
      661 catgatgggt tttctgcgcg ggctggattg cggtaagaac ggtgaaacca ctgtaatgat
      721 aggcaattca ggtaataaaa aagccggagc tccctttccg gcacgtctca ttgccgtatc
      781 acttcctccc gaaaaagcat taatcagtaa aacccgactg ctcagcgaga atcgtcgaaa
      841 aggacgagta gttcaggcgg aa
//
LOCUS       DAPHBJ010000419          854 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.422, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000419 DAPHBJ010000000
VERSION     DAPHBJ010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.422"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            80..712
                     /locus_tag="RHZ76_004641"
     CDS             80..712
                     /locus_tag="RHZ76_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2815121.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT          279 a          149 c          165 g          261 t
ORIGIN      
        1 attacaaaat catggtgtat tgacatcatc aatcaccaat gaaattatgc aattatatac
       61 ggatagggag gttcttaaca tggggctttg tagtcgttat aaaagtctta catgcaatag
      121 ttgctcaatg cattgccaaa taatgccaga agagtcaccg cgtttgcagt actgcgcaaa
      181 ctcgtgtttt tgtatgtggc ccgaagaaag ttcatatttt aatcgtggtg tggtagaagg
      241 tatcttaaca aaaaaccaca atgcgagatt aagcggatat atttttgtcg atttttcagt
      301 aagtttttta cgtctattcc ttgaaaaaga ctggattgac tatcttgcaa gtactgacat
      361 ggggattgtt ttagtcagcg accgcaatat gcaatcgtta gctaattact ggcgaaaaca
      421 caactcagca atatctgctg tcatatataa tgatgatggt cttgatgttg ccaatgaaaa
      481 gatcagacaa ctatttattg gtcgttattt atcatttacc agaggtaaca cattaactca
      541 gatggaattc accataatgg ggtatatggt ttctggttat aatccatatc agattgctga
      601 agttctggat atggatatcc gtagcatcta cgcgtacaag caacgaatcg aaaagagaat
      661 gggtggtaaa ataaacgaat tatttattcg ttcacattcg gtccaacatt gatactcaac
      721 gaccagccag aatcatactc tgtttatacg ggcatttatt gaaatgtccg tattttataa
      781 ataggccgtc ccctcaagag taaacaccat agtatttaaa accatcacac gaaaaattca
      841 gaagcattac gaaa
//
LOCUS       DAPHBJ010000420          823 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.423, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000420 DAPHBJ010000000
VERSION     DAPHBJ010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.423"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            44..823
                     /locus_tag="RHZ76_004642"
                     /pseudo
     CDS             44..823
                     /locus_tag="RHZ76_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT          272 a          161 c          160 g          230 t
ORIGIN      
        1 actatgtgat ctccatttcg attgatttag tgtttattga cgtatgtact ggattattaa
       61 cgataatatc gagttctggc ctgagcaccg aaaattaata tcggtacata acgccgatct
      121 taacgtcgtt ctgacaacgc cagccagtcg atgtttatca cttctacttg aagcttttcc
      181 tgatgtggtt gcacaacaag attttttcac cagagtctgg gaagaagaag gtatgcgtgt
      241 gcctactaac acgttatatc agaacatatc cattatcaga cgcggatttc gcgctgttgg
      301 tgatactacc cactcgctaa ttgcaaccgt gccgagaaga ggattcaaga tccataatga
      361 cattaacata caaaatcatg taataaactc gtcaacagac gcacatacac acaatgcccc
      421 acctgccata aaagttaatg cgggatacaa agagagcatt ggtggcgcaa agaatttcaa
      481 taacaaaatc ctcaaacata taaaaagtca tctaattatg ttgagcgcat ttgtcatagg
      541 cgcatattct gcatattggc tatggaataa taatcaaccg aagccatttt tcaaggatta
      601 caagattgtt gcggaaatta atggctgaca ttttaatgta acagaagata caattgatgg
      661 gttgaaagag ttcgataaat ataaaacacg gatactggat tcggggatta actgtaaaaa
      721 acatccgtgg ttatacttcc ctcttgcaaa atcctcaccg gggatgattg ttatggcatg
      781 caataaaaac tataaccaac atgaagtggc agactgttta acc
//
LOCUS       DAPHBJ010000421          819 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.424, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000421 DAPHBJ010000000
VERSION     DAPHBJ010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.424"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..319)
                     /gene="sctI"
                     /locus_tag="RHZ76_004643"
     CDS             complement(<1..319)
                     /gene="sctI"
                     /locus_tag="RHZ76_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HDV2815122.1"
                     /translation="MSVSNMPPIDRAEQSTAHEIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALRSDKKGLTPEKLLVLQDHVLNYNVEVSLVGTLARKIVS
                     AVET"
     gene            complement(339..578)
                     /locus_tag="RHZ76_004644"
     CDS             complement(339..578)
                     /locus_tag="RHZ76_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
                     /protein_id="HDV2815123.1"
                     /translation="MADWNGYIMDISKQFDQGVDDLNQQVEKALEVLATNPSDPKFFA
                     EYQSALAEYTLYRNAQSNVVKAYKDLDSAIIQNFR"
     gene            complement(592..>819)
                     /locus_tag="RHZ76_004645"
     CDS             complement(592..>819)
                     /locus_tag="RHZ76_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HDV2815124.1"
                     /translation="KYKTANNVYFNIIGTLSDSELNKINNYVDEYYKQWGKQYVRFNV
                     NLKNQDTNNSSFSYGDNRFEKSQGSNWTFQE"
BASE COUNT          233 a          166 c          126 g          294 t
ORIGIN      
        1 acgtctcaac tgcagagaca atctttcttg ctaatgttcc caccaatgaa acctcgacat
       61 tataattaag cacatgatcc tgcaagacta ataatttttc aggagttaac ccctttttat
      121 cagaccttaa tgcttgtaac gcattttgct gccaattttc agtttgtaca gcatagttgg
      181 caaaagcgga gatcatttta tcatccgggt tgtcaagatt gagaacccgg tcatttaaat
      241 caatcacttt cgcttgctgt atttcatgag cagttgattg ctcagctcta tcaataggtg
      301 gcatattgct tactgacata ataaaatcct tatccagttt agcggaagtt ttgtatgatt
      361 gctgaatcaa gatctttata cgcttttaca acgttagatt gcgcatttcg atataatgta
      421 tactcagcta atgcactttg atattcggca aaaaatttcg ggtcggaggg attggttgct
      481 aaaacctcca acgctttttc aacttgctgg ttcagatcat caacgccctg atcaaattgt
      541 ttgctgatat ccataatata accattccaa tctgctataa gtcctcctca attattcctg
      601 aaacgtccag ttgctacctt gagacttctc gaatcggtta tctccgtagc taaatgaact
      661 attatttgtg tcctgatttt tcaaattcac attaaatctt acatattgtt taccccattg
      721 tttataatat tcgtcaacat agttattaat tttatttaat tcactgtcac ttaacgtacc
      781 aatgatattg aaatacacat tgttagctgt tttatattt
//
LOCUS       DAPHBJ010000422          813 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.425, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000422 DAPHBJ010000000
VERSION     DAPHBJ010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.425"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            178..780
                     /gene="fimB"
                     /locus_tag="RHZ76_004646"
     CDS             178..780
                     /gene="fimB"
                     /locus_tag="RHZ76_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HDV2815125.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          234 a          157 c          184 g          238 t
ORIGIN      
        1 tggcaaagtt attataagta actatcgcta ctccatttaa gttcactcag aagaactggt
       61 ccacttacgt tagttattaa gcaaacgttc gcttttataa acataatcag gattaaaatg
      121 ttggattatt gctaacccag cacagctagt gcgcgtctgt aattataagg gaaaacgatg
      181 aagaataagg ctgataacaa aaaaaggaac ttcctgaccc atagtgaaat cgaatcactc
      241 cttaaagcag caaataccgg gcctcatgca gcacgtaatt attgtctgac tttgctttgt
      301 tttattcatg gtttccgggc gagtgaaatt tgtcgattga ggatttcgga tattgatctt
      361 aaggcaaagt gtatctatat ccatcgatta aaaaaaggct tttcaacaac gcacccgcta
      421 ttgaacaaag aagttcaggc tttaaaaaac tggttgagta tccgtacttc gtacccgcat
      481 gctgagagcg aatgggtatt tttatcgcgt aaggggaatc cgctttctcg gcaacagttt
      541 taccatatta tctcgacttc cggtggtaat gccgggttgt cactggagat tcatccgcac
      601 atgttacgcc attcgtgtgg ttttgctttg gcgaatatgg gaatagatac gcgacttatc
      661 caggattatc ttgggcatcg caatattcgt catactgtct ggtataccgc cagcaatgca
      721 gggcgttttt acggcatctg ggatagggcc agaggacgac agcgtcacgc tgttttatag
      781 agatagttga gataccaggg atggtgttaa aaa
//
LOCUS       DAPHBJ010000423          802 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.426, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000423 DAPHBJ010000000
VERSION     DAPHBJ010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.426"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            11..>802
                     /locus_tag="RHZ76_004647"
     CDS             11..>802
                     /locus_tag="RHZ76_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2815126.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDSSVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNRHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEI"
BASE COUNT          249 a          174 c          147 g          232 t
ORIGIN      
        1 ggagtagtgt atggccaatc tctacgactt gaaaaagttc gacttaaatc ttcttgtcat
       61 atttgagtgt atttaccagc atttaagcat tagcaaagct gctgaatcac tatatatcac
      121 cccatcagcc gtgagccagt cgttacaacg cttacgtgcg caatttaatg accctttatt
      181 cattcgctca ggaaaaggta tcgcgccgac gacaacaggg ttaaatctgc atcaccatct
      241 tgagaaaaat ctcagaggcc ttgaacaaac aattaatata gtcaataagt ccgaactaaa
      301 gaaaaacttt attatctacg gccctcagct catttcttgc tctaataata gtatgctgat
      361 ccgctgccta cggcaagatt cctctgtcga gattgagtgc catgatatcc tcatgtcagc
      421 agagaatgct gaagaactgt tggttcaccg caaagccgat ctggtgatca cccaaatgcc
      481 agtgatcagc cgttccgtca tttgcatgcc attgcatacg attcgcaata ctctaatttg
      541 tagtaatagg catccacgaa ttactgacaa tagcacttat gaacaaatta tggcagaaga
      601 gttcacccaa ttaatatcta aatcagcagg ggttgatgat attcaaatgg aaatagacga
      661 gagatttatg aatcgcaaaa tctcatttcg tggttcctct ttgctgacaa tcattaatag
      721 cattgcagtt acagatttac tgggaatagt cccctatgaa ctttataatt cctaccgtga
      781 ctttcttaac ttaaaagaga ta
//
LOCUS       DAPHBJ010000424          798 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.427, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000424 DAPHBJ010000000
VERSION     DAPHBJ010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.427"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(17..688)
                     /gene="queE"
                     /locus_tag="RHZ76_004648"
     CDS             complement(17..688)
                     /gene="queE"
                     /locus_tag="RHZ76_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDV2815127.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAIIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTYDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          202 a          205 c          177 g          214 t
ORIGIN      
        1 gcttataaat gtttaatcag gcaatattta aatatttatg ggtttgcatc gacaaacgcc
       61 aattacgcgc aatacaggtt tcaatgcaca aacgtgtggc atcatccttt tggctaatcg
      121 gctgcagtgc aatgactcgc ggtttatcat aggtcagcgt tgccagtagt tcatccagtg
      181 cttcaatatc gcgtacgcgc cccactggat gcttgatttc gttggctcgc tccagtgcct
      241 gtgacaacac ttcatagccg ccgcgcatgt tcagctttgg cgatacggta acccaggtat
      301 tcggtgtgca gcgtacttca tgagtaccac tggtttcgat ctggcagcta aaaccgttct
      361 tttcgagcag atcagtcagt ggtagcaaat catgaatgca aggctcacca cccgtaatca
      421 ccacatgccg cgcggtgtat ccctggcgac caatgatagc cagcaagtct tcactgctcg
      481 ccgcccccca cttatcactc tctttggtct tcgccagaat gctgaaaagg gagacttccc
      541 gatcctcaag cttttcccag gtgtgtttgg tgtcgcacca ggcacagcca accgggcatc
      601 cctgtaaacg aataaaaatg gcgggaacgc cggtaaagta accctcacct tgcagggtct
      661 ggaacatctc gttaatcggg tactgcatag cattctctgt gaagtggata attgttaatt
      721 attgcagatc ctgccacaac aatcatgtct tattaacatt ctgttacatg cagatttaaa
      781 gaaaaatgcg aaacatat
//
LOCUS       DAPHBJ010000425          794 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.428, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000425 DAPHBJ010000000
VERSION     DAPHBJ010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.428"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..711
                     /locus_tag="RHZ76_004649"
     CDS             <1..711
                     /locus_tag="RHZ76_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDV2815128.1"
                     /translation="ATILRDQGVDIILNAHVERISHHENQVQVHSEHAQLAVDALLIA
                     SGRQPATASLHPENAGIAVNERGAIVVDKRLHTTADNIWAMGDVTGGLQFTYISLDDY
                     RIVRDELLGEGKRSTDDRKNVPYSVFMTPPLSRVGMTEEQARESGADIQVVTLPVAAI
                     PRARVMNDTRGVLKAIVDNKTQRILGASLLCVDSHEMINIVKMVMDAGLPYSILRDQI
                     FTHPSMSESLNDLFSLVK"
BASE COUNT          224 a          172 c          193 g          205 t
ORIGIN      
        1 gcgacgattt tacgcgatca gggcgtcgat attatcctca atgcccatgt ggagcgaatc
       61 agtcaccatg aaaatcaagt gcaagtgcat agcgagcacg cccaactggc ggtggatgca
      121 ctgttaatag cttccggtcg tcaaccggct accgcttcgt tacatccaga aaatgccggt
      181 atcgcagtaa acgagcgcgg ggcaattgtc gttgacaagc gattacatac caccgcagac
      241 aatatttggg cgatgggaga tgttaccggc gggctgcaat ttacttacat atcactggat
      301 gattaccgca ttgtacgtga tgagttactg ggtgaaggca aacgtagtac tgatgatcgg
      361 aaaaatgtgc cttattccgt atttatgaca ccgcccctgt ccagggttgg tatgacagaa
      421 gaacaagcca gagagagtgg tgctgatatt caggtggtga cattgcctgt agctgcaatt
      481 ccgcgcgcca gagtgatgaa cgatacccgt ggggtattaa aagcgattgt tgataataaa
      541 acccaacgta tattaggggc atcactgctg tgtgttgact cccacgagat gatcaatata
      601 gtgaaaatgg tgatggatgc cgggctgcct tatagcatat tacgcgatca gatatttact
      661 catccgtcga tgagcgaatc actcaatgat ctattttcat tagtcaaata aactcataat
      721 cagacgccag aacaaatatt ctggcgcctc agagaaaaga atcttattaa ttctctgtta
      781 ctctataact tctt
//
LOCUS       DAPHBJ010000426          793 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.429, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000426 DAPHBJ010000000
VERSION     DAPHBJ010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.429"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            46..336
                     /locus_tag="RHZ76_004650"
     CDS             46..336
                     /locus_tag="RHZ76_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDV2815129.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTTLRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANDSRGKNL"
     gene            360..>793
                     /locus_tag="RHZ76_004651"
     CDS             360..>793
                     /locus_tag="RHZ76_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HDV2815130.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNTLESI"
BASE COUNT          287 a          156 c          133 g          217 t
ORIGIN      
        1 tcgtttagaa aatacgttta acgataatta aaaggattct gttaaatgaa attaaaaata
       61 gttgcggtgg ttgtaactgg tttgttagct gcgaacgtag cacacgctgc cgaagtctat
      121 aacaaggatg gtaataaact cgacctttat ggcaaggtta ccactctacg ttattttact
      181 gatgataagc gtgacgatgg tgataaaact tatgcccgtc tcggctttaa aggagaaacg
      241 caaatcaatg atcaaatgat tggttttggt cactgggaat atgattttaa aggctataac
      301 gatgaagcca acgactcgcg cggcaagaat ctctgattga gttaagtgcc catcacaata
      361 tgatcactga ccttatatta cacaatcatc ccaggatgaa aacaatcact ttaaacgaca
      421 accatattgc acatttaaac gccaaaaaca ctacaaaact ggaatattta aacttaagca
      481 ataacaattt actgccaacc aatgacattg atcaactaat atcatcaaaa catctttggc
      541 atgtattagt taacggcatc aacaatgatc cacttgcaca aatgcagtac tggactgcag
      601 taagaaatat aattgatgac actaatgaag tgaccattga tttatcagga cttaatttaa
      661 ccactcaacc accagggctg caaaacttca cctctatcaa tcttgataat aaccaactca
      721 cacattttga tgcaaccaac tacgatagac tcgtaaagct aagtctgaat agtaatactc
      781 ttgagtcaat aaa
//
LOCUS       DAPHBJ010000427          791 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.430, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000427 DAPHBJ010000000
VERSION     DAPHBJ010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.430"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(387..>791)
                     /locus_tag="RHZ76_004652"
     CDS             complement(387..>791)
                     /locus_tag="RHZ76_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2815131.1"
                     /translation="TQIQEHLAMGVLLSLGAHHVNCLLLSDSKQSLTSRCYNLMLSEP
                     GTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNALSAIQTTVKPISEIA
                     RENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT          237 a          165 c          139 g          250 t
ORIGIN      
        1 atagttaaaa taaaagggct cacgatgaaa aaattaacag tggcaatttc tgctgtagct
       61 gcatcttaac gtataaacta gatttaataa caacgatatc cgtacctatc cggatatcta
      121 atgtcataag catcccttta attatcccct caacctcttg taactttccc actcttgaac
      181 tccacatagt ccaaacgccg gatgcaaaaa aatcacacgt gcatttctga aattgtgatg
      241 gaggaagata gtatcaatta tatgtgctta taaacgtccc cgattagcgc agaagtcacc
      301 ggattgttca ggctccaata ggttgagtat gccgtattta ttttccaaaa taaaaatcga
      361 tgctgttgct ccttcataaa atacttttac tctctggacg cttttcttag ctctcttggt
      421 gttatcttaa aacgattatg gaacctttca gtaaaacgag aaggacactt gtaaccattt
      481 tccctggcaa tctcgctgat gggttttact gtcgtttgta tagcagacaa cgcattattt
      541 aacctcacgt cgtccaatat actttgaaaa cttactccct cgcttgccag acggcgatgc
      601 aatgtggaaa cagaaatgta gagatatctc gctaccttgt ttgctgtcca ttttgttcca
      661 ggttcggata gcattaggtt ataacaacga cttgttaatg attgtttact atctgataaa
      721 agtaaacaat taacatgatg tgctcctaat gaaagtagaa cgcccattgc taagtgttcc
      781 tgaatttgcg t
//
LOCUS       DAPHBJ010000428          783 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.431, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000428 DAPHBJ010000000
VERSION     DAPHBJ010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.431"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     repeat_region   1..697
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1..26
                     /rpt_unit_seq="ttccccgcgccagcggggataaaccg"
BASE COUNT          180 a          233 c          221 g          149 t
ORIGIN      
        1 ttccccgcgc cagcggggat aaaccgctct tcagcaatga aatcgtcaaa cgagattaga
       61 gttccccgcg ccagcgggga taaaccgcac aattattcgg tacgacgggt tcaggcattg
      121 agttccccgc gccagcgggg ataaaccgga ccagagatgt cgtagccgta tttcgcagcc
      181 gagttccccg cgccagcggg gataaaccgt caccgggtca gatactgatg ttatggctta
      241 tgagttcccc gcgccagcgg ggataaaccg aggagtttaa tttccagatt gagcgctgga
      301 tagagttccc cgcgccagcg gggataaacc gggcacaaaa aaaaccgcgc acggcgggtt
      361 aatgagttcc ccgcgccagc ggggataaac cggtgagtcc gtcagcggtg cgccgctgca
      421 acacgagttc cccgcgccag cggggataaa ccgtaaggcc gtcgccggat cagcctggct
      481 atgccgagct ccccgcgcca gcggggataa accgttcttg cgggtgttgc aaatattctt
      541 cacgtagagc tccccgcgcc agcggggata aaccggagcc tgacgagact actgaggccg
      601 ttctgtcgag ttccccgcgc cagcggggat aaaccggacg ccgccgccgc gaagccgttt
      661 ccgatgttga gttccccgcg ccagcgggga taaaccacca tataacccgt tatctctttc
      721 tcaagttttt atattagcag tacttgtaat aagcaacata tccacgtaac acctcatgtt
      781 caa
//
LOCUS       DAPHBJ010000429          780 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.432, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000429 DAPHBJ010000000
VERSION     DAPHBJ010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.432"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..695
                     /gene="ydeO"
                     /locus_tag="RHZ76_004653"
     CDS             <1..695
                     /gene="ydeO"
                     /locus_tag="RHZ76_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HDV2815132.1"
                     /translation="AFSDGEILMVDNAVRTHFEPYERHFKEIGFTENTIKKYLQCTNI
                     QTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFAACKGFI
                     TLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQTTFSQILL
                     DARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYRCQSHTG
                     MNTGNTMSALAI"
BASE COUNT          232 a          160 c          163 g          225 t
ORIGIN      
        1 acgccttctc tgacggcgag atcctgatgg tagataacgc tgttcgtacg cattttgaac
       61 cttatgagcg gcattttaaa gagatcggat ttactgaaaa taccattaaa aaatatctac
      121 aatgcactaa catccagaca gtgacggtgc ctgttcctgc gaagttttta cgtgcttcaa
      181 atgtaccgac tggattgctt aatgaaatga ttgcttatct caactcggaa gaacgcaatc
      241 atcataattt ttcagaactt ttgctttttt cttgcctgtc tatttttgcc gcatgcaaag
      301 gtttcattac actattaact aacggtgtgc tatccgtttc cggaaaagtg agaaatattg
      361 tcaacatgaa gctggcgcac ccatggaagc tgaaagatat ttgtgactgc ctgtacatca
      421 gtgaaagcct gttgaagaaa aaactaaagc aagagcaaac gacattctca cagattctat
      481 tagatgccag aatgcagcac gcaaaaaatt tgatacgcgt agaaggttca gtcaataaaa
      541 ttgccgaaca atgtggttat gccagtacat cttattttat ttatgcgttc cgcaaacatt
      601 tcggcaacag tccgaagaga gtttctaagg agtaccgttg tcaaagtcac acgggtatga
      661 atacgggcaa cacgatgagt gctttagcta tttgattatt tgctaacgag tagtcaacca
      721 cgcacgctac gtaagaatta tatggggcag ccattccctg ccccgcgttg tttttaggcg
//
LOCUS       DAPHBJ010000430          764 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.433, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000430 DAPHBJ010000000
VERSION     DAPHBJ010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.433"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..764
                     /locus_tag="RHZ76_004654"
                     /pseudo
     CDS             1..764
                     /locus_tag="RHZ76_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          253 a          146 c          173 g          192 t
ORIGIN      
        1 attttgaaaa tggtattcct gttcacgata ccattgccag agttgtatcc tgtatcagtc
       61 ctgcaaaatt tcaccagtgc tttattaact ggatgcgtga ctgccattct tcagatgata
      121 aagacgtcat tgcaattgat ggaaaaacgc tccagcactc ttatgacaag agtcgccgca
      181 ggggagcgat tcatgtcatt agtgcgttct caacaatgca cagtctggtc atcggacaga
      241 tcaagacgga tgagaaatct aatgagatta cagctatccc aaaacttctt aacatattgg
      301 atattaaagg aaaaatcatc acaactgatg cgatgggatg ccagaaagat attgcagaga
      361 agatacaaaa atagggcggt gattatttat tcgcggtaaa aggaaaccag gggcggctta
      421 ataaagcctt tgaggaaaaa tttccgctga aagaattaaa taatccagag catgacagtt
      481 acgcaatcag tgaaaagagt cacggcagag aagaaatccg tcttcatatt gtttgcgatg
      541 tccctgatga acttattgat ttcacgtttg aatggaaagg gctgaaaaaa ttatgcgtgg
      601 cagtctcctt tcggtcaata atagcagaac aaaagaaaga acccgaaatg acggtcagat
      661 attatatcag ctctgctgat ttaactgcag agaagttcgc cacagcaatc cgaaaccact
      721 ggcacgtgga aaataagctg cactggcgcc tggacgtggt aatg
//
LOCUS       DAPHBJ010000431          756 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.434, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000431 DAPHBJ010000000
VERSION     DAPHBJ010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.434"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            91..>756
                     /locus_tag="RHZ76_004655"
     CDS             91..>756
                     /locus_tag="RHZ76_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDV2815133.1"
                     /translation="MTLITQENEHDRLATRLSIILSRLFQGEKLHIGTLAEEFGVTTR
                     TLRRDFNIRLTYLDIEQNNGVYQLASHYFRRRTERDMKILARLLHLDRLLPAMDAKLL
                     SLLLDGERPSPYRIMLPEPRQKPTLFGDFSRLTLAILNQTQVRIRTDENVSHTVHPYR
                     LLCSHAEWFLAGCTSSGVFVISLSRIRLVEVLPDTTFEIDNTLISLIEQSDFMEALPH
                     MNII"
BASE COUNT          184 a          203 c          183 g          186 t
ORIGIN      
        1 aggtattcag actttaaaaa acttcagagg gcggacaaat cctgtccgct actgctggca
       61 tcatttcccg gctaaattcg gaaatgatgt atgacgctga taacacaaga aaatgaacat
      121 gacagactgg caacccgcct ttccatcatc ctgagccgtt tgtttcaggg tgaaaagctg
      181 catatcggga cgctggcaga agaatttggg gtgacgacgc gcacgctgcg gcgggatttc
      241 aacatccgcc tcacctatct tgatattgaa cagaacaacg gcgtatatca gctcgcttcg
      301 cattactttc gtcgccgcac ggaacgggat atgaagatac tggcgaggtt gctgcatctt
      361 gaccgtctgt tacccgcgat ggacgccaaa ctgctgagcc ttctgcttga cggggagcgt
      421 ccctcgccat acaggattat gctaccggag ccgcgccaga aacccaccct tttcggtgat
      481 ttcagcagac tgacgctcgc catacttaac cagacccagg tgcgtatcag aaccgacgaa
      541 aacgtcagcc ataccgttca tccctaccgt ctgctttgca gccatgcgga gtggtttctt
      601 gccggatgta cgtcatcggg cgtgttcgtt atttccctgt cccgaatcag gctggttgag
      661 gtattaccgg atacaacgtt tgagatcgat aacacactca tttcgctgat tgaacagtct
      721 gactttatgg aagccttacc ccacatgaac attatt
//
LOCUS       DAPHBJ010000432          749 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.435, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000432 DAPHBJ010000000
VERSION     DAPHBJ010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.435"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(173..715)
                     /gene="fimA"
                     /locus_tag="RHZ76_004656"
     CDS             complement(173..715)
                     /gene="fimA"
                     /locus_tag="RHZ76_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDV2815134.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVIAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT          223 a          159 c          154 g          213 t
ORIGIN      
        1 agaaatgatt gctttggttt cttacaacac atcattccct tttcatttaa taaagcggca
       61 ggatacaaag tgaacatgat ttcgatttac attgccacca taccgcttca gacaaccggt
      121 acattacttt gttataagac gtgatatata attcaaaaca acgtggtttt gattattcgt
      181 atctcataat aaaagtcgcg tcagcatttg cttgccctgc tgatgcactt gtaccggtgc
      241 ctttataacg tgcagaaaaa tgaagaacgt tggtcccggg gatcaagttc tggttggttg
      301 agaagctatt cccatcaggt ttgagaattg cggatgtatt atcaagtatt tcaataccga
      361 cacccgttgc tgttgttgta ttggtactgg atgcaatggc caataaatta tcattgattg
      421 catcagcctg accagaaaat gcaacggcag cattagcggc aataacagga tcgcagtcgt
      481 tcaactgaat ggtgaatgga attaatgcag aggtattacc aacagcattg aaaatatcgg
      541 tacgatattg accgagtgtg acaacctggt ctgccgagtc agtattcact gaacaggcag
      601 cattcaccag ttcgccttca aagtgaattg taccgccatc tacaacggca gcataactac
      661 ccgtagcggc gaatagtgcg gcagccagcg cagacgaaat aaatcttaat ttcatatata
      721 ttccttcaat ctcatttatc gactccaca
//
LOCUS       DAPHBJ010000433          744 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.436, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000433 DAPHBJ010000000
VERSION     DAPHBJ010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.436"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            41..>744
                     /locus_tag="RHZ76_004657"
     CDS             41..>744
                     /locus_tag="RHZ76_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV2815135.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEEL
                     VDALEDDDDVQKVYHNV"
BASE COUNT          246 a          130 c          164 g          204 t
ORIGIN      
        1 ttaccagtaa tgtaaataaa ttttcgagga gatcattcca gtgggacgta aatgggccaa
       61 tattgttgct aaaaaaacgg ctaaagacgg tgcaacgtct aaaatttatg caaaattcgg
      121 tgtagaaatc tatgctgctg ctaaacaagg tgaacccgat ccagaattaa acacatcttt
      181 aaaattcgtt attgaacgtg caaagcaggc acaagttcca aagcacgtta ttgataaagc
      241 aattgataaa gccaaaggcg gcggagatga aacgttcgtg cagggccgtt atgaaggctt
      301 tggtcctaat ggctcaatga ttatcgccga gacattgact tcaaatgtta accgtacgat
      361 tgctaacgtt cgcacaattt tcaataaaaa aggcggcaat atcggagcgg caggttctgt
      421 cagctatatg tttgacaata cgggtgtgat tgtatttaaa ggatcagacc ctgaccatat
      481 ttttgaaatt ttacttgaag ctgaagttga tgttcgtgat gtgactgaag aagaaggtaa
      541 cattgttatt tataccgaac ctactgacct tcataaagga atcgcggctc taaaagcagc
      601 tggaatcact gagttctcaa caacagaatt agaaatgatt gcgcaatctg aagttgagct
      661 ttccccagaa gatttagaaa tctttgaaga gcttgttgat gcccttgaag atgacgacga
      721 tgtacaaaaa gtttatcata acgt
//
LOCUS       DAPHBJ010000434          742 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.437, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000434 DAPHBJ010000000
VERSION     DAPHBJ010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.437"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            11..>742
                     /locus_tag="RHZ76_004658"
     CDS             11..>742
                     /locus_tag="RHZ76_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDV2815136.1"
                     /translation="MAKQIIQGTTEINSLGIKNHFESVEPVQAILELVWNGFDAGAKN
                     ININIVYDHFDTVKNLTILDDGDGINFTDLGSNFNRFNDSLKKTSIDQHGSKGRGRLS
                     FHRLCHQAIWYTKYLGRNSYIKINDEDIKAYEAQIDIDADEQNSLLNAMDKGTCVELT
                     LLHSSLPDRRELISILSNEFCCKLALDDERKVFVNGAEVDIPEHEIKEVSFEIENNDF
                     SVKGIRWDKKPATEKSFIYLTDSSGK"
BASE COUNT          255 a          111 c          160 g          216 t
ORIGIN      
        1 gtgagcgcaa atggcaaagc agattattca gggcacaact gaaatcaaca gcttgggtat
       61 caagaaccat tttgagtcag ttgaacctgt acaggcgata ttggaactcg tatggaatgg
      121 atttgatgct ggcgctaaga atattaacat aaatatagtt tatgaccatt ttgatacagt
      181 aaaaaacctg accattcttg atgatggtga tgggataaac tttactgatt taggcagcaa
      241 ctttaatcga tttaacgatt cattaaaaaa aacaagcatt gatcaacacg gctctaaagg
      301 tcgagggcgt ttatcatttc atagactttg tcatcaggct atctggtata ctaaatactt
      361 aggtagaaat tcctatatca aaattaatga tgaggatatt aaggcatatg aagctcaaat
      421 agatatcgac gctgatgagc aaaacagttt attgaatgca atggataagg ggacgtgtgt
      481 tgaattaaca ttgttacatt caagccttcc agatagacgc gaactgattt ctattttgtc
      541 caatgaattc tgttgtaaat tggcattgga tgatgaaagg aaggttttcg taaatggtgc
      601 tgaagttgac atcccagaac acgaaataaa agaggtttcc ttcgagattg aaaacaatga
      661 tttctctgtg aaagggattc gctgggataa aaagccagcg acagaaaaat catttattta
      721 cttgactgat agttcaggaa ag
//
LOCUS       DAPHBJ010000435          731 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.438, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000435 DAPHBJ010000000
VERSION     DAPHBJ010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.438"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..593)
                     /locus_tag="RHZ76_004659"
     CDS             complement(<1..593)
                     /locus_tag="RHZ76_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDV2815137.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPRE"
BASE COUNT          207 a          167 c          139 g          218 t
ORIGIN      
        1 tcttcccgag gcaaaaacag tgaagctgct tctaaaatgg ttaccttgct gccaaaatta
       61 gcgaacatag aggcgaactc aacgccaata tatccgccgc ccaaaatacc taaatgccca
      121 ggcaattctt ttagattaag taatccggtg ctgtcatata ctcctggcgt ggtggtaatt
      181 ccaggaattg gcggaaccac ggtttgtgca ccggtattaa taaaaatttt ctcgccatga
      241 atttccagat ttccctcagg ccgatgaaca cgcaggctat gattattgat aaactccgcc
      301 tggccgtcga tcacgtcgat attgggcata tccgcaagat tatgaaaatt cttattacgt
      361 aaaaaattaa ccacttcatt tttacgctgt atggcacgga caaaatctgt gtgctgctgt
      421 gcgtcatgaa ccaatgtttt ggttgggatg cagccgatat taatacaggt cccgccatac
      481 attgcatttg attgttcgat gagagccact cgccaacctg cttttgccag cgtgacggct
      541 aatgtttttc cagccttgcc aaaaccaata atcactgcct gatatttatt catgatgatt
      601 tcctgtcgaa acgcgttcat ttgacatcat gactatagac gtaaaagagc aaataacatt
      661 tcttcacaac gcttgatatt ttgtcttatc gtctggtatt ttctttttct taatcagaat
      721 cacaaaacaa a
//
LOCUS       DAPHBJ010000436          718 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.439, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000436 DAPHBJ010000000
VERSION     DAPHBJ010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.439"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..709
                     /locus_tag="RHZ76_004660"
     CDS             <1..709
                     /locus_tag="RHZ76_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HDV2815138.1"
                     /translation="LAAFTRSKQRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAK
                     ASRKFSPVSYRAHGLPVSENLLEQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLW
                     SRAVIGWSMSPRMTAQLACDALQMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHN
                     LRGSMSAKGCCYDNACVESFFHSLKVECIHGEHFISREIMRATVFNYIECDYNRWRRH
                     SWCGGLSPEQFENKNLA"
BASE COUNT          165 a          182 c          215 g          156 t
ORIGIN      
        1 cctcgcggct tttacccggt caaaacagcg ttacggtgcc ccacgcctga cggatgaact
       61 gcgtgctcag ggttacccct ttaacgtaaa aaccgtggcg gcaagcctgc gccgtcaggg
      121 actgagggca aaggcctccc ggaagttcag cccggtcagc taccgcgcac acggcctgcc
      181 tgtgtcagaa aatctgttgg agcaggattt ttacgccagt ggcccgaacc agaagtgggc
      241 aggagacatc acgtacttac gtacagatga aggctggctg tatctggcag tggtcattga
      301 cctgtggtca cgtgccgtta ttggctggtc aatgtcgcca cgcatgacgg cgcaactggc
      361 ctgcgatgcc ctgcagatgg cgctgtggcg gcgtaagagg ccccggaacg ttatcgttca
      421 cacggaccgt ggaggccagt actgttcagc agattatcag gcgcaactga agcggcataa
      481 tctgcgtgga agtatgagcg caaaaggttg ctgctacgat aatgcctgcg tggaaagctt
      541 ctttcattcg ctgaaagtgg aatgtatcca tggagaacac tttatcagcc gggaaataat
      601 gcgggcaacg gtgtttaatt atatcgaatg tgattacaat cggtggcggc ggcacagttg
      661 gtgtggcggc ctcagtccgg aacaatttga aaacaagaac ctcgcttagg cctgtgtc
//
LOCUS       DAPHBJ010000437          683 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.440, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000437 DAPHBJ010000000
VERSION     DAPHBJ010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.440"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>683
                     /locus_tag="RHZ76_004661"
     CDS             <1..>683
                     /locus_tag="RHZ76_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDV2815139.1"
                     /translation="VAGIPFVVSFVGLGRLFGNKRGWLNKFIFNSVLSIYSLLLSAKS
                     PAQVIFEHNADFAELSKYIKFDENNIHIIEGAGVDRFRFSYQPEPTQNNFSVLFASRL
                     LWSKGLGEVVEAIGKVKQNENNTTLYVAGILDDKDPDRIELDQILQWEKEGKIIWLGR
                     RDDIQNLIRNSHVVILPTKYSEGVPRIIIEACAMGRSCIVGNVPGCKAIIKDNFNGCV
                     LKTHSANEI"
BASE COUNT          225 a          109 c          156 g          193 t
ORIGIN      
        1 gggttgcagg catccctttt gtcgtgagct ttgtgggatt agggagattg tttggcaata
       61 aacgaggttg gttgaataaa ttcattttca actcagtact gtcaatttat agtcttttgc
      121 tatcagcaaa aagcccagcg caagtaatat ttgagcataa tgctgacttt gctgagctga
      181 gtaaatatat taaatttgat gaaaataaca tccacatcat tgaaggagcc ggggtagaca
      241 ggtttcgctt tagttatcag ccagaaccaa ctcagaataa tttctcggta ttatttgcca
      301 gccgtttgct atggtcaaag gggctcggtg aagtagtcga ggccatcggt aaggttaaac
      361 aaaatgaaaa taacacaacc ttatacgttg cgggaattct cgatgacaaa gatccggata
      421 gaattgaatt agatcaaatt ttgcaatggg aaaaagaagg aaaaatcatt tggttaggtc
      481 gaagagatga catccaaaat ttaatcagaa atagccatgt cgtgattttg ccaactaaat
      541 attcagaagg cgtgccaaga ataatcattg aagcgtgtgc tatgggccga agttgcattg
      601 tcggaaatgt acccggctgt aaagccataa ttaaagataa tttcaatgga tgtgtactaa
      661 aaacacatag cgctaatgag att
//
LOCUS       DAPHBJ010000438          683 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.441, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000438 DAPHBJ010000000
VERSION     DAPHBJ010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.441"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..580)
                     /gene="fimE"
                     /locus_tag="RHZ76_004662"
     CDS             complement(<1..580)
                     /gene="fimE"
                     /locus_tag="RHZ76_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HDV2815140.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKL"
BASE COUNT          202 a          172 c          140 g          169 t
ORIGIN      
        1 ttaatttttc gtttataaga ttatttcttt cccataatcc ggcaaaacga gcagcattac
       61 tggcggtata acgcacagta tggcgaatat ttcgatgccc gagataatcc tgaattaaac
      121 gggtatctgc accacgctcc gccagttcat aaccgcaagc atgccttaac atatgaggat
      181 gagtctgcgt tacggttcca gcttcaatac cggcatcgcg aataatgcga taggcctgct
      241 ggcgagaaag ccgactcccg cggcgagaaa taaatatagc gtcggtccgg tcagcgcctt
      301 tccagttagc acgttcctgg gtccagcgtt ccacggcttc acgctcatca aaacgtaacg
      361 ggtgaacggt agaaaatccg ttcttcagtc ggcgaatatt tattcgacct tcattaaggt
      421 caaggtcctg ataatgcaga tcaagcagtt cactaatacg catcccatgc cgatatgcca
      481 acagaataag acaataatct ctggctcccg ttgccccgta acaaaccgcc tgcatcatgg
      541 cctgaacttc tttaccggta agataacgac gtttactcac aatagtagta ctcctgactg
      601 agatatattt gaatgaatac ctataggaaa cctcaatcgg tcaaaattag cccaatagat
      661 aaaaagataa ccacacacga agt
//
LOCUS       DAPHBJ010000439          648 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.442, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000439 DAPHBJ010000000
VERSION     DAPHBJ010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.442"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..104
                     /locus_tag="RHZ76_004663"
     CDS             <1..104
                     /locus_tag="RHZ76_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815141.1"
                     /translation="WKIEGKQPKTLGGNFTILIAKKDGQVLHVIHTK"
BASE COUNT          204 a          124 c          143 g          177 t
ORIGIN      
        1 gttggaaaat cgaggggaaa caaccgaaga cgttaggagg aaattttaca atactaatag
       61 cgaaaaaaga tgggcaagtg ttgcatgtaa tacacacaaa ataatccact gtacagtcga
      121 ttcaaatatt ttaccaaggt gtgtttcgta actattttgt gtacaatatg actctacata
      181 tcgacgaaaa aaccacttga tttgacagga acacattcaa aaaatttctt ttttcatttc
      241 aggagcgatt ttcatctgtt acttgaagta tctgtgagat taagtgactc cccctaacgt
      301 cgtactaaat agtaattact ggccataccg ggagcagtat tcggcgcttc ccacatgcag
      361 ctgaataacc gcatcagcga agatgtattg tatttttact atcacaatgt gtacggacgg
      421 tttatggaaa agaatgaaca ttagatacgc gatgcggcgg caggttacac ggttatcact
      481 acgatagcca atactggctg gtgcattacc atcgtgaaca ataggacgcc gtactgctgg
      541 aaatttctta cctttatgac tccccgcgcc gccgtatcgg aaaacgggaa gcaatcaaga
      601 tgaggctgat ataatgtgcg ggatgcctga gtatttttag aatagggg
//
LOCUS       DAPHBJ010000440          627 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.443, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000440 DAPHBJ010000000
VERSION     DAPHBJ010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.443"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>627)
                     /locus_tag="RHZ76_004664"
     CDS             complement(<1..>627)
                     /locus_tag="RHZ76_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815142.1"
                     /translation="RSSELLFNFYECCYKFLPRAQPQDKIDSYNSALQAFSIFCSSTL
                     THNNIGFDFKLFPEVKLSGEHLETVFKYKNGDDVREIAKINITLQKEEGGLYNLRGLD
                     FKGCFFSGQNFSNYDIQYVNWGTSLFDVDTPCIFNAPAYNKSNEKSLKPVSENGLSGV
                     LTDRNNKIKLITGVAPFDDILFMDDDFDDSSSEDDPVENSPVVTSPVVS"
BASE COUNT          210 a          128 c           99 g          190 t
ORIGIN      
        1 tgatacaacg ggactagtca caacaggact attctcaacg ggatcatcct cagaggaact
       61 atcatcaaag tcatcatcca taaataaaat atcatcgaat ggtgccacgc ccgtgatgag
      121 ttttatttta ttattacgat cagtcaatac tccacttaaa ccgttttcgc tcacaggttt
      181 taatgatttt tcattactct tgttgtaagc aggcgcatta aaaatacacg gagtatcaac
      241 atcaaacaat gacgttcccc agttcacata ttgaatatca tagttactga agttctgtcc
      301 agaaaagaag caccccttaa aatccaatcc acgtaaatta tataaaccac cctcttcttt
      361 ttggagagta atgttaattt tggctatctc ccggacatca tcgccatttt tgtatttgaa
      421 taccgtttca agatgttctc cagacagctt gacttcagga aataatttga aatcaaagcc
      481 tatattatta tgtgtcaacg tagatgaaca aaaaatggaa aaagcttgca gtgctgaatt
      541 atagctatcg attttatctt gaggctgagc tcttggtaaa aacttatagc aacactcata
      601 aaaattaaac agaagctctg aagacct
//
LOCUS       DAPHBJ010000441          612 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.444, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000441 DAPHBJ010000000
VERSION     DAPHBJ010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.444"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(78..440)
                     /locus_tag="RHZ76_004665"
     CDS             complement(78..440)
                     /locus_tag="RHZ76_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HDV2815143.1"
                     /translation="MIANPTHIAIGIYFKPHLSPIPLISVRETNEVALAVRKYAKEIG
                     IPIITDKKLARKIYATHRRYDYVSFENIDEILRLLLWLEDVENAGQPVPDEQLSSEDK
                     FIEGEEKEIENKDDNLKN"
     gene            complement(467..>612)
                     /locus_tag="RHZ76_004666"
     CDS             complement(467..>612)
                     /locus_tag="RHZ76_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HDV2815144.1"
                     /translation="FIAEYFLFMKDMKMDKQEVKREYKEQEGNPEIKSKRRERIRKFF
                     LSN"
BASE COUNT          169 a          115 c           93 g          235 t
ORIGIN      
        1 tcgtttaact cattaaataa tgaatctaca cggtttttgc gtgtagattc ttataaattt
       61 ctgactacga cagatgttta attttttaaa ttatcgtctt tattctcgat ttctttctct
      121 tcaccttcaa tgaatttatc ttctgaagag agctgttcat ctggaacagg ttgtccagca
      181 ttctccacat cttcaagcca cagcagaaga cgtaatattt catctatatt ttcgaagcta
      241 acataatcgt agcgacgatg ggtagcataa atttttcgtg ctaatttttt atctgtgata
      301 attggtattc cgatttcctt tgcatatttc cttacggcta aggctacctc attagtttct
      361 cttacagaaa tcaatggaat aggtgaaaga tgtggcttaa agtatatccc tattgcaatg
      421 tgagtagggt tggcaatcat caaacgacta ttactgacat cagacttcaa ttgctcagaa
      481 agaatttcct gatgcgctct ctgcgtttag acttaatttc tggatttcct tcttgctcct
      541 tgtattctct tttaacttct tgtttatcca ttttcatatc tttcataaat aaaaaatatt
      601 cagcgataaa at
//
LOCUS       DAPHBJ010000442          610 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.445, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000442 DAPHBJ010000000
VERSION     DAPHBJ010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.445"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            8..574
                     /gene="slp"
                     /locus_tag="RHZ76_004667"
     CDS             8..574
                     /gene="slp"
                     /locus_tag="RHZ76_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HDV2815145.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          169 a          139 c          142 g          160 t
ORIGIN      
        1 tagtaacatg aacatgacaa aaggtgcact catcctcagc ctttcatttt tgcttgccgc
       61 atgtagttca attccgcaaa atatcaaagg caataaccaa cctgatattc aaaaaagttt
      121 tgttgctgtt cataaccagc cggggttata tgttggtcaa caagcgcgct ttggtgggaa
      181 ggttatcaac gttatcaatg gcaaaacgga tacgttgtta gaaatcgctg tattaccgtt
      241 ggatagctat gcgaagcctg atattgaagc caactatcag ggccgactgc tcgccagaca
      301 aagcggcttc cttgatccag tgaactatcg taatcacttt gttaccatcc tcggcaccat
      361 tcagggtgaa caacctggct ttatcaataa agtcccgtat aacttcctgg aagtgaatat
      421 gcagggcatc caggtgtggc atttgagaga agtggttaat accacctata acctgtggga
      481 ttacggctat ggtgcattct ggccggaacc gggctggggt gcgccttact acaccaatgc
      541 ggtgagtcag gtaacacctg agctggtcaa ataacaccac cgaaagatgc agatgctttc
      601 tcagcatctg
//
LOCUS       DAPHBJ010000443          592 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.446, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000443 DAPHBJ010000000
VERSION     DAPHBJ010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.446"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..441
                     /locus_tag="RHZ76_004668"
     CDS             <1..441
                     /locus_tag="RHZ76_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDV2815146.1"
                     /translation="IHSSLELSFILHKYHLRVDEKKSRNVFSAIRDELILDGDSRQNL
                     ATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRMLSDSWSFPDSRHTLG
                     CSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANMLA"
     gene            422..>592
                     /locus_tag="RHZ76_004669"
     CDS             422..>592
                     /locus_tag="RHZ76_004669"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDV2815147.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKAD"
BASE COUNT          171 a          108 c          129 g          184 t
ORIGIN      
        1 attcatagct cacttgagct ttcatttata ttgcataaat atcatttgcg tgtagatgag
       61 aagaaatctc gaaatgtatt cagcgccata cgtgatgagc tcatccttga tggggattca
      121 cgacaaaact tagctacttt ctgtcaaaca tggcttgatg atgaaatccg tgagttgctt
      181 aatttagcta ttgataaaaa catgatttat aaagatgaat atccacaaat ggcggaacct
      241 gaaggtcgct gtgtgcgtat gctttccgat tcgtggagtt tccccgattc gcgacacacc
      301 ttaggttgct caaccattgg ttcatctgaa gctgctatgc tcggccgctt ggcgttaaaa
      361 tggcagtggt gtaaaaaacg cgaagtacaa ggtaaatcaa ccgaacctga tttgcgatcc
      421 tgtgcaaata tgctggcata aattctgccg ttactgtgat gtgaaattgt gtgaaattcc
      481 acttgatgat catcacctga taatgaatgc taaagaggtt ttgaagcatg tcgatgaaaa
      541 cactattggt gtcgttacga ctttaggtat tacttatttt tgtaaagcag at
//
LOCUS       DAPHBJ010000444          591 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.447, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000444 DAPHBJ010000000
VERSION     DAPHBJ010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.447"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>591
                     /locus_tag="RHZ76_004670"
     CDS             <1..>591
                     /locus_tag="RHZ76_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815148.1"
                     /translation="YHEKVVMNYMEYLVTCQLDEEQMSSLLYERDDLSAIDFSLLMKT
                     IKAFSFGGDLQTLASKPGSTISSIPSKRRILININHDFPNNGNLFNDFLFNHQQDEQL
                     AMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGDEAWLNKGCSTYQQLYNEFH
                     ALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHS"
BASE COUNT          186 a          113 c          117 g          175 t
ORIGIN      
        1 aatatcatga aaaagtcgtt atgaattaca tggaatatct ggtaacttgt cagttggatg
       61 aagaacaaat gtcatcgctg ctttatgaaa gagatgattt aagcgccatt gatttttcat
      121 tgctcatgaa gacgataaaa gctttttcat ttggtggcga tctccaaacc cttgcatcaa
      181 aaccgggttc cacaatctca agcatcccat caaaaagacg gatattgatt aacattaacc
      241 acgattttcc taacaatggc aatcttttca atgactttct atttaaccat caacaagatg
      301 aacaattagc aatggcatat atggccgctc tcccgttcag caggccttta gtttactggg
      361 atggccaagt attaaaatca acgactgaaa ttaaaaatta tgatgggtcg acgcgtgttg
      421 gcgatgaggc gtggcttaat aaaggttgct ctacctatca gcagctctac aatgaattcc
      481 acgcattata tatagataaa gcaggaatat ggagcgcatt tgagggtgta tttgcaacta
      541 aaaacgttct ggcctttagt cgtggggatt ctgtgaacat taatcactct c
//
LOCUS       DAPHBJ010000445          579 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.448, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000445 DAPHBJ010000000
VERSION     DAPHBJ010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.448"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>579)
                     /locus_tag="RHZ76_004671"
     CDS             complement(<1..>579)
                     /locus_tag="RHZ76_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HDV2815149.1"
                     /translation="DHSTIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNG
                     RWAYLYRAVDSRGRTVDFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYG
                     RALALLKREGRCPSDVEHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGI
                     EVMRALRKGQASAFYYGDPLGEMRLVSRVFEM"
BASE COUNT          128 a          155 c          154 g          142 t
ORIGIN      
        1 tacatttcaa aaactctgct taccaggcgc atttcgccca ggggatcacc ataataaaat
       61 gctgaggcct ggcctttgcg tagtgcacgc atcacctcaa tacctttgat ggtggcgtaa
      121 gccgtcttca tggatttaaa tcccagcgtg gcgccgatta tccgtttcag tttgccatga
      181 tcgcattcaa tcacgttgtt ccggtactta atctgtcggt gttcaacgtc agacgggcac
      241 cggccttcgc gtttgagcag agcaagcgcg cgaccatagg cgggcgcttt atccgtgttg
      301 atgaatcgcg ggatctgcca cttcttcacg ttgttgagga ttttacccag aaaccggtat
      361 gcagctttgc tgttacgacg ggaggagaga taaaaatcga cagtgcggcc ccggctgtcg
      421 acggcccggt acagatacgc ccagcggcca ttgaccttca cgtaggtttc atccatgtgc
      481 cacgggcaaa gatcggaagg gttacgccag taccagcgca gccgtttttc catttcaggc
      541 gcataacgct gaacccagcg gtaaatcgtg gagtgatcg
//
LOCUS       DAPHBJ010000446          562 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.449, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000446 DAPHBJ010000000
VERSION     DAPHBJ010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.449"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(1..230)
                     /locus_tag="RHZ76_004672"
                     /pseudo
     CDS             complement(1..230)
                     /locus_tag="RHZ76_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
     gene            complement(273..509)
                     /locus_tag="RHZ76_004673"
     CDS             complement(273..509)
                     /locus_tag="RHZ76_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HDV2815150.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQEILGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
BASE COUNT          157 a          124 c          100 g          181 t
ORIGIN      
        1 ttgtcagctg tcgctgttct tcggtttggc cctcctgaat cactattgct aaaatcacga
       61 attatgctgt caattaaatc gcgtcggaaa gcatcttcct cagcctattt cattacgagt
      121 aattgcttaa taagagtctg caatgaaaaa tttatcttca tgcgcagtat ctggagcacc
      181 gcctggccag taatttcata ttttatacgt tcattatcag atgtcttcat aacgacttca
      241 tagctaacct gttaaataat caggctgttg cattattcaa aaagcaaccc aattagtgca
      301 ttataatgtt tctgttcctc ttcccctgtc gcttgttcca gtcggcaaag aagtttggca
      361 caaagcgact ttcgactcag attacgtcca tctttcaaaa tctcagtcac aatttccccg
      421 agaatttcct gctgagtagg caagttagct tttgcaaagt aacgagtgat tgctcccgca
      481 gaatctgaag tcactgaatt ttgatgcatg ctacgcctct gttaaaaatg ttaatcaatg
      541 ttaagtgagg taacaatagc aa
//
LOCUS       DAPHBJ010000447          549 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.450, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000447 DAPHBJ010000000
VERSION     DAPHBJ010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.450"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..514
                     /locus_tag="RHZ76_004674"
     CDS             <1..514
                     /locus_tag="RHZ76_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2815151.1"
                     /translation="FSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFEAGGDSVSINM
                     PTVPTSVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGIKLSLITNDKITGNDKAIAS
                     SNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQSGDVL
                     SSLVMRVAQD"
BASE COUNT          170 a           95 c          120 g          164 t
ORIGIN      
        1 cttttcttct ttttttatga gcgctggagt atttgctgca gacgttgata ccggaacatt
       61 aactattaag gggaatattg cagaatctcc gtgtaaattc gaagcgggtg gtgattcagt
      121 aagtattaat atgccgactg taccaaccag tgtctttgaa ggtaaagcta aatattctac
      181 ctatgatgat gcagtcggtg taaccagcag catgttaaaa attagctgcc cgaaagaagt
      241 tgctggtata aaactctcgt tgattaccaa cgataaaata accggtaacg ataaggcgat
      301 agccagtagc aacgataccg tgggttacta tctctattta ggtgataaca gcgatgtcct
      361 ggatgtttct gcacctttta acattgagag ttataaaaca gcggaaggtc aatatgctat
      421 tccgtttaaa gcaaaatacc tgaaactgac agataactca gtgcaatcag gtgatgtgtt
      481 atcttctctg gttatgcgtg tggcgcagga ttaataaata aaattgtaag tttctccagg
      541 taataaaca
//
LOCUS       DAPHBJ010000448          549 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.451, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000448 DAPHBJ010000000
VERSION     DAPHBJ010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.451"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(1..395)
                     /locus_tag="RHZ76_004675"
                     /pseudo
     CDS             complement(1..395)
                     /locus_tag="RHZ76_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
BASE COUNT          183 a           97 c           93 g          176 t
ORIGIN      
        1 ttttacggtt tttaagccaa atatacgctt taagccattt actggattca atgaatcaaa
       61 cttaaattta acagcttcag ttgctaattg cattttactt tgaaaccagg agacaagtat
      121 tgttgtgaac atgcaaacca gcaaaaaagg aatgatcgtt ttcaaccaac caacccccat
      181 agctttaaaa tagtcccaaa tatctggttt agcgttatga tcaaggatgt attcaagaat
      241 cgacataatg tgatgcacat caaaaacata tccgagatat aaagtaccca caagcattat
      301 tacagagatg gttaagtctc tactttttag aatttgccct tttttagaag catcctggag
      361 tttctttgtg ttggcttttc agttttattg gccatgaaac actctatttc tcaatgtata
      421 gttgcaattg ttctgggaaa aaggataacg gcattactcg ttcagccagt attgggccaa
      481 aataaataat taatattaaa aatgccagcg cacttttgac agtcagtgaa atggcaaaag
      541 catttagtt
//
LOCUS       DAPHBJ010000449          545 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.452, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000449 DAPHBJ010000000
VERSION     DAPHBJ010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.452"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>545)
                     /locus_tag="RHZ76_004676"
     CDS             complement(<1..>545)
                     /locus_tag="RHZ76_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HDV2815152.1"
                     /translation="INSICNPEDNKDATPIERYTFDVETKNGLPSTVQLFYEESKDNE
                     PLANIHLQAIGSGFLTFVNACQEYDDNSLKLFASLLISLSYSSAYTDLAGAEKVNIND
                     DNENYLTAQFEELSQRDMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGKLAPDILS
                     KMRDAAPKLIDFAKSFDPNSK"
BASE COUNT          159 a          118 c           88 g          180 t
ORIGIN      
        1 tcctttgagt ttgggtcaaa cgacttagca aagtcaatta atttcggtgc agcatctcgc
       61 attttgctta aaatatcagg ggcgagcttt ccttcattca ccagatgtga catcttatcc
      121 agatagccat caaaattcat ttctccccca tctgccagac gcttcatctc tcccaggtac
      181 ttcttcatat cacgttgaga taactcttca aactgagctg tcagatagtt ctcattatca
      241 tcattaatat tcactttttc tgctcctgct aaatctgtgt aggcactaga gtatgaaagt
      301 gaaatcaaca gcgaagcgaa taattttaag ctgttgtcat catattcctg acatgcatta
      361 acaaacgtta aaaaaccaga acctattgct tgcaaatgta tattcgctaa aggttcatta
      421 tctttagatt cctcataaaa aagctgaacc gtggagggaa ggccattctt ggtttctaca
      481 tcaaaagtat atcgttctat gggcgttgca tctttattat cctcaggatt acatatacta
      541 ttaat
//
LOCUS       DAPHBJ010000450          543 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.453, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000450 DAPHBJ010000000
VERSION     DAPHBJ010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.453"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..505
                     /locus_tag="RHZ76_004677"
     CDS             <1..505
                     /locus_tag="RHZ76_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV2815153.1"
                     /translation="AECNKSTAQIFILGEFAGGMMGSELVKWVKSHKIDAHIITFVAK
                     MPYIDSIKLLEAGAKGCVWKTSHPAKLNRAIDSISNGYTYFDSVHMDCEKISSRYSSD
                     NQLKNRESEILQLIADGKTNKEIANFLQLSRKTVETHRLNIMKKLDVHSGIELIKTAL
                     RMGVCTI"
BASE COUNT          192 a           83 c          111 g          157 t
ORIGIN      
        1 agcggagtgt aacaaatcta cagcccaaat atttatttta ggagaatttg caggtggaat
       61 gatggggtcc gaactcgtaa aatgggttaa atcgcataag atagatgctc atattattac
      121 atttgtagca aagatgccgt atattgattc aataaaattg cttgaagcag gtgcgaaagg
      181 atgcgtatgg aaaaccagtc acccggcgaa actaaatcgt gctattgatt cgattagtaa
      241 tggttacact tattttgata gtgtacatat ggattgtgaa aaaatatctt ccaggtattc
      301 ctctgataat caacttaaaa atcgtgagtc agaaatatta caactcatag ctgatggtaa
      361 aacaaacaaa gaaatcgcta atttcttgca gttaagcagg aaaacagtcg aaactcatag
      421 actcaatatc atgaagaaat tagatgttca cagtggtatc gagttgatca aaacagcttt
      481 acgtatgggt gtttgcacta tataaaaaaa agaggcttta ggctcatttt gctgaagcct
      541 tac
//
LOCUS       DAPHBJ010000451          540 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.454, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000451 DAPHBJ010000000
VERSION     DAPHBJ010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.454"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>540
                     /locus_tag="RHZ76_004678"
     rRNA            <1..>540
                     /locus_tag="RHZ76_004678"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          125 a          121 c          181 g          113 t
ORIGIN      
        1 gggtagtttg actggggcgg tctcctccta aagagtaacg gaggagcacg aaggttggct
       61 aatcctggtc ggacatcagg aggttagtgc aatggcataa gccagcttga ctgcgagcgt
      121 gacggcgcga gcaggtgcga aagcaggtca tagtgatccg gtggttctga atggaagggc
      181 catcgctcaa cggataaaag gtactccggg gataacaggc tgataccgcc caagagttca
      241 tatcgacggc ggtgtttggc acctcgatgt cggctcatca catcctgggg ctgaagtagg
      301 tcccaagggt atggctgttc gccatttaaa gtggtacgcg agctgggttt agaacgtcgt
      361 gagacagttc ggtccctatc tgccgtgggc gctggagaac tgaggggggc tgctcctagt
      421 acgagaggac cggagtggac gcatcactgg tgttcgggtt gtcatgccaa tggcactgcc
      481 cggtagctaa atgcggaaga gataagtgct gaaagcatct aagcacgaaa cttgccccga
//
LOCUS       DAPHBJ010000452          539 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.455, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000452 DAPHBJ010000000
VERSION     DAPHBJ010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.455"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>539
                     /locus_tag="RHZ76_004679"
     rRNA            <1..>539
                     /locus_tag="RHZ76_004679"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          151 a          110 c          171 g          107 t
ORIGIN      
        1 tgtgtgttag tggaagcgtc tggaaaggcg cgcgatacag ggtgacagcc ccgtacacaa
       61 aaatgcacat gctgtgagct cgatgagtag ggcgggacac gtggtatcct gtctgaatat
      121 ggggggacca tcctccaagg ctaaatactc ctgactgacc gatagtgaac cagtaccgtg
      181 agggaaaggc gaaaagaacc ccggcgaggg gagtgaaaaa gaacctgaaa ccgtgtacgt
      241 acaagcagtg ggagcacgct taggcgtgtg actgcgtacc ttttgtataa tgggtcagcg
      301 acttatattc tgtagcaagg ttaaccgaat aggggagccg aagggaaacc gagtcttaac
      361 tgggcgttaa gttgcagggt atagacccga aacccggtga tctagccatg ggcaggttga
      421 aggttgggta acactaactg gaggaccgaa ccgactaatg ttgaaaaatt agcggatgac
      481 ttgtggctgg gggtgaaagg ccaatcaaac cgggagatag ctggttctcc ccgaaagct
//
LOCUS       DAPHBJ010000453          537 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.456, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000453 DAPHBJ010000000
VERSION     DAPHBJ010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.456"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>537)
                     /locus_tag="RHZ76_004680"
     CDS             complement(<1..>537)
                     /locus_tag="RHZ76_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HDV2815154.1"
                     /translation="SFVFHGTGERYFLICVVNVLLTIITLGIYLPWALMKCKRYLYAN
                     MEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVDCVLTLSLLVLLIFMA
                     AKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTVFFISTKMLHANSS
                     SSVIISVVLMAIVGIVSIG"
BASE COUNT          219 a          126 c           74 g          118 t
ORIGIN      
        1 accaatggaa acaataccaa ctattgccat cagaaccaca gatataataa cactacttga
       61 actattggca tgtagcatct ttgtcgagat aaagaaaaca gtccccatcc caatggccat
      121 taaaatcggc aagaaaaagg ttacccacca gaaccctttc aaagagcaat taaaactaaa
      181 tcttacgccg ttgagactag tcatcaaggc ctgataacgc agtccttttg ctgccataaa
      241 tataagcaaa accaacagtg acaaagtcaa aacacagtca acaagcggca tatctgctga
      301 cactgtcatt aagattgcga aatagaaaaa aacaaaaaca agacaactaa caaaaacatt
      361 cccaccggta atcccataag aaaatcgttg tccgttaact tccatattag catagagata
      421 acgcttacat ttcattaatg cccatggtaa atagatacct agcgttataa tcgttaacaa
      481 cacattcacc acacaaataa gaaaatatct ctcacctgta ccatgaaaaa caaacga
//
LOCUS       DAPHBJ010000454          535 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.457, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000454 DAPHBJ010000000
VERSION     DAPHBJ010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.457"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>535)
                     /locus_tag="RHZ76_004681"
     CDS             complement(<1..>535)
                     /locus_tag="RHZ76_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815155.1"
                     /translation="SQYLKLGDDQSPVQLDPHSEVGAFKVVGHCAWAKPEDKITPDFL
                     RSRVEPWLTALFQSEHLNILIGAGLSSAIQESATGTKPQGMGWIEDLKVCKAEIDSYV
                     AKTADASGRGKGNIEDQIRSINELIKGLEILTVQNLPVPPSPPGAPSYRNLKSELVEL
                     NNELTRCLKLFSDSVSYG"
BASE COUNT          144 a          121 c          102 g          168 t
ORIGIN      
        1 ccataactaa ctgagtctga aaataatttc agacaccttg ttagctcatt atttaactct
       61 actaactcac tttttaaatt tcgatatgag ggagcacctg gtggagatgg tggaaccggt
      121 aggttttgta ccgttaaaat ctctagtcct ttaatcaact cattaattga acgaatctga
      181 tcttcaatat taccctttcc ccttcctgag gcgtctgctg ttttagctac ataactgtct
      241 atttcagcct tacaaacttt gagatcttca atccatccca taccttgagg tttggtgccg
      301 gtcgctgact cttggatagc cgaactaagg ccggcgccaa taagtatatt taagtgttca
      361 gactgaaaaa gtgcagttaa ccaaggttct actcttgaac gcaaaaagtc aggagtgatt
      421 ttatcttctg gttttgccca tgcgcagtgt ccaacgactt taaatgcacc aacttcgctg
      481 tgtgggtcca actgaaccgg agactggtca tcgccaagct ttaaatattg actca
//
LOCUS       DAPHBJ010000455          519 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.458, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000455 DAPHBJ010000000
VERSION     DAPHBJ010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.458"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>519
                     /locus_tag="RHZ76_004682"
     CDS             <1..>519
                     /locus_tag="RHZ76_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HDV2815156.1"
                     /translation="GQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYGYQL
                     NPISDIDPLGLSMWEDAKSGACTNGLCGTLSAMIGPDKFDSIDSTAYDALNKINSQSI
                     CEDKEFAGLICKDNSGRYFSTAPNRGERKGSYPFNSPCPNGTEKVSAYHTHGADSHGE
                     YWDEIFSGKDEK"
BASE COUNT          164 a           98 c          126 g          131 t
ORIGIN      
        1 cggggcagca gtatgatgag gagtccggcc tgtattacaa ccgccaccgc tattatgacc
       61 cgctgcaggg gcgatatatc actcaggatc cgattggact gaagggggga tggaacctgt
      121 atggatatca attgaatccg atatcagaca tcgaccccct gggtttatct atgtgggagg
      181 atgcaaaatc gggggcatgt actaatggtc tttgcggcac actatccgct atgataggtc
      241 cagataaatt tgattctata gatagcaccg catatgacgc cttaaataaa ataaatagcc
      301 aatctatttg cgaagataaa gagttcgctg gtttaatatg taaggataat agtggcagat
      361 atttctcaac agcacctaac cgaggagaaa gaaaaggatc atatccattc aatagccctt
      421 gccctaatgg tactgagaaa gtatcagctt atcatactca tggtgcagat agtcatggag
      481 aatattggga cgaaatattt tcaggtaaag atgagaaaa
//
LOCUS       DAPHBJ010000456          509 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.459, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000456 DAPHBJ010000000
VERSION     DAPHBJ010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.459"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(11..178)
                     /locus_tag="RHZ76_004683"
     CDS             complement(11..178)
                     /locus_tag="RHZ76_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815157.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(181..345)
                     /locus_tag="RHZ76_004684"
     CDS             complement(181..345)
                     /locus_tag="RHZ76_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HDV2815158.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
BASE COUNT          151 a          114 c          103 g          141 t
ORIGIN      
        1 atgatgataa ttattgtttc ggctgatcat cttcagcaaa cagttctata aaggcttgct
       61 gctgcgatgt tagttgccag tcgtctggaa taacgacgga acattcccgt ttcgtttctg
      121 tttcattgtc actatgctgg cataatggtg acgactccag ttgctcctga ccgaccatag
      181 ttatccccac actttccaaa tcagcagtgc gacaaccacc cagaaaagtg cagaaccaag
      241 aaaaaccgcc agccaggctt tacgttttaa ttcaggatcg cggcggggtt cctgatttcc
      301 agaaggcatt gctaacctca tataatcgcc atcacttatc agcaagacgt tgaacgattg
      361 gtgtaattaa tcatacgatg agacaaatcc taaagaaact ttagtcaaaa atccagcgaa
      421 tttacgaatc gtctccagtg aataattcaa tcttttgacc tgaaatagat atttgaaaac
      481 tggagtttgc gaggcgggta attagacac
//
LOCUS       DAPHBJ010000457          509 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.460, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000457 DAPHBJ010000000
VERSION     DAPHBJ010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.460"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..509
                     /locus_tag="RHZ76_004685"
                     /pseudo
     CDS             1..509
                     /locus_tag="RHZ76_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
BASE COUNT          161 a           98 c           89 g          161 t
ORIGIN      
        1 gtggctttta ttaatcacct tgagtctata ttcttttatt gtgcaaagtg ctccctgtgc
       61 attgaccaat gtcggggaac aacgtggaac atatatactc aagccactga gtatgaaagg
      121 taatctcacc gccgaaaaaa ttaaaaacac cggaaatatc attgagtgtg cagacttaca
      181 agagacgacc aacgccatta aagttcattt tttaacacag aacgctttaa ggtttaaggt
      241 ttaaagtcgg aagtcgtcat tatattatta atttcccctt tgaatctgac ggtaaaaaaa
      301 tagcgcttcc agggaagtca tcttataatt tagaagaaat gctatctgcg ataaatttca
      361 ccattccata ctctatcgca tctgtatcag atagttccaa caccgcagat atttttccag
      421 gaactccttt tcctttgttg ttcgaaattg aagtgagttc atgtacaggg aataatacta
      481 attgtaatag cgcccgcttt aattttaat
//
LOCUS       DAPHBJ010000458          508 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.461, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000458 DAPHBJ010000000
VERSION     DAPHBJ010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.461"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT          153 a           85 c           99 g          171 t
ORIGIN      
        1 tagaaatgtc catatgttga aaaggccgct gcgaaaatct ctcccgcagc ggcctcttta
       61 gcataacgtt attgtgcgaa ccatcattct gatttaacgg tgtttatgct actttttctg
      121 tatgtctctc tatgtacatg cggcaggtca aaaagtgaac attattgaat ctaaaattgc
      181 tgggttaggg ttcctacagg cgatcatagc tagaatggct aataattcgt tttatattaa
      241 aggatgggac ctaacattat ttgttgcagt tgttgcgtta aaaaaggatg catcccaata
      301 ttcaggttat ttacttcttg cgttaatagt gagcaccata gcgttctgtt tattggatgc
      361 ttattatcta caacaggaga ggattttcag gaagatatat aattacttgg ctgaatcaaa
      421 ttcagaatct attaactatt ttagcataaa ccctgtgcta tacaaagata tactaaaggg
      481 tgaaatgctt tcatatagaa gctcatta
//
LOCUS       DAPHBJ010000459          505 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.462, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000459 DAPHBJ010000000
VERSION     DAPHBJ010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.462"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(155..>505)
                     /locus_tag="RHZ76_004686"
     CDS             complement(155..>505)
                     /locus_tag="RHZ76_004686"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815159.1"
                     /translation="SKINIKNSAKFNIRSTSRLTYDIISQLHLPQNGYFMERSNFLVA
                     IIMLILRSLAIIPAWFVSLGHEIVTFPFILEIWGNAGDAFSSTSVGNYGFGHYDLNLP
                     QSQLFAGRYGKQTK"
BASE COUNT          141 a           87 c          119 g          158 t
ORIGIN      
        1 ctcagtgagc ttggtgaaaa taagtgttta cccgccatca ggctgagtat aattctcatc
       61 atgaaatatg tttcttggtt tgtggcttgt aactggtcac tcctgatgtc gatctggaga
      121 ggcttgttga tgttggtgtt ttcaggatga tgtttcactt agtttgtttg ccgtatcgcc
      181 cggcgaatag ctgtgattga ggaaggttta agtcgtagtg accaaagcca tagttaccaa
      241 cggatgtaga tgaaaaagcg tcgcctgcgt tcccccatat ctccaggata aaagggaatg
      301 taacaatctc atggcctaag ctgacgaacc aggcaggaat aatcgctagg gatctaagaa
      361 ttagcatgat aatagccact aaaaaattac tgcgttccat gaaatagcca ttttgtggca
      421 aatgcagttg actaataatg tcatatgtga gacggctagt tgaacgaata ttaaattttg
      481 ctgaattttt tatgttgatt ttact
//
LOCUS       DAPHBJ010000460          500 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.463, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000460 DAPHBJ010000000
VERSION     DAPHBJ010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.463"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>500
                     /locus_tag="RHZ76_004687"
     CDS             <1..>500
                     /locus_tag="RHZ76_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815160.1"
                     /translation="AWCEHFSRFPRPFKWIDLNEEDNVLWLWDYMRKNHIGCDVLPSS
                     SKQRKYFLLATFDNWAGWTNEQFSKQKENARKVRANPEEPSSLKKLLNPTTPDDCRKV
                     FLDYARNAWVQRRHRQKVKKDTTGIKLTAQSRKKLLAIQQLSGVEPNAILKSLIDDAY
                     KRMKEK"
BASE COUNT          171 a           93 c          114 g          122 t
ORIGIN      
        1 gcgtggtgcg agcattttag tcgattccct cgcccattta agtggattga tttaaacgaa
       61 gaagataatg ttctctggtt gtgggactat atgcggaaaa atcatattgg atgcgatgtg
      121 cttccatcat cgtctaagca aagaaaatat tttttgctgg caacgtttga taactgggcc
      181 gggtggacga atgaacagtt tagtaaacaa aaagaaaatg cccgtaaagt gagggctaac
      241 cccgaagagc cgtcttcgct aaagaagcta ttaaatccca ctacccccga tgattgcaga
      301 aaagttttcc tggactatgc acgtaatgcc tgggtgcaga gacgacatcg tcagaaagta
      361 aaaaaagata caaccgggat aaagcttaca gcacaatcca gaaagaaact tttggctata
      421 cagcagttat caggtgttga gccaaatgcc atactaaaaa gcctcattga tgatgcttat
      481 aaaagaatga aggaaaaaaa
//
LOCUS       DAPHBJ010000461          493 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.464, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000461 DAPHBJ010000000
VERSION     DAPHBJ010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.464"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            103..>493
                     /locus_tag="RHZ76_004688"
     CDS             103..>493
                     /locus_tag="RHZ76_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815161.1"
                     /translation="MGGTGERSGPDIWGNPFYHQYLCVPDGKGDYTCGGQDQRGESKG
                     DGLWGPGKASNDTKEAAGRCDLVETDNSCVENCLKGKFKEVRPRYSVLPDIFTPINLG
                     LFKNCQDWSNDSLETCKMKCSGNNIGRF"
BASE COUNT          159 a           78 c          124 g          132 t
ORIGIN      
        1 ctgaaagggg gatggaattt ttatcagtat ccgttgaatc cgatctcaaa tatagatcca
       61 ttaggattag aaacactaaa atgcattaag ccactgcatt caatgggcgg aactggtgaa
      121 agaagcggtc cagatatatg ggggaatccg ttctatcatc aatatctttg tgtcccagat
      181 ggtaaagggg actatacttg tggtggccaa gaccaacggg gagaatcaaa aggagatggt
      241 ctatgggggc caggtaaagc aagtaatgat acaaaagaag ctgctggccg ttgtgacctc
      301 gttgaaaccg ataatagttg tgtggagaac tgtttaaaag ggaagtttaa agaggtaagg
      361 ccgcgttatt ctgtattgcc tgatatattc acacctataa atttagggct atttaaaaac
      421 tgccaagact ggtctaatga ttctttagaa acatgtaaga tgaagtgctc cggaaataac
      481 attggacgtt tta
//
LOCUS       DAPHBJ010000462          485 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.465, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000462 DAPHBJ010000000
VERSION     DAPHBJ010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.465"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(416..>485)
                     /locus_tag="RHZ76_004689"
     CDS             complement(416..>485)
                     /locus_tag="RHZ76_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HDV2815162.1"
                     /translation="QLINVNFFSSDRTSFCECNRFP"
BASE COUNT          140 a           97 c           94 g          154 t
ORIGIN      
        1 tacttatact gtaaatttta ttaacgtcat tggtggagac aatttctgca aactttcttt
       61 ttaccgtcca ataatcaata tttctattgt gattgttaac cactgcgtgc attggcggga
      121 ttgaagggtg aaaaatatgc gcaattggga agcctttcat tgatgcctgc cttgtcatca
      181 tgacaaacaa taatagatgc ttattttcct cgcagggttc tttcgcacta tcaaaaaacg
      241 ctcatttacc cggcgttaca gtaaacgaag caacattctg tggcctacgc cgatcaaaag
      301 ctctgtgaaa atttcccttg ttgtctttgt ctgaccttat cgcggcgatc agcaattatt
      361 cgttttgtga tcctcaacac aaaatgctat tcgtcgccta tgaggggaga agaatttatg
      421 gaaaacgatt gcattcacaa aatgacgtcc tgtcagaaga gaaaaagttg acgttaatca
      481 attgt
//
LOCUS       DAPHBJ010000463          484 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.466, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000463 DAPHBJ010000000
VERSION     DAPHBJ010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.466"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            18..368
                     /locus_tag="RHZ76_004690"
     CDS             18..368
                     /locus_tag="RHZ76_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000928911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2815163.1"
                     /translation="MLMNEDTKRQTDMKNAALRAADVLRGLANSDRLLLMCQLSLGEA
                     NVAGLEAAVGITQPTLSQQLGVMRRLKLVETRRAGKMVYYRIEDNRIMTLLNTLYDLY
                     CPQAGTGKGSHNNE"
BASE COUNT          134 a          115 c          133 g          102 t
ORIGIN      
        1 agtatgagaa atcagcaatg ctcatgaatg aagacacaaa acgtcagaca gatatgaaga
       61 atgcggccct gagagcagca gatgtcttac ggggactggc caacagcgac cggttgctgt
      121 tgatgtgtca gttaagcctg ggggaagcga atgtggccgg gctggaagcg gcggtcggta
      181 tcactcagcc gaccctgtcc cagcagctgg gtgtaatgcg ccgcctgaag ctggtcgaga
      241 cccgacgggc aggtaaaatg gtatattacc gtattgagga taaccgtatc atgacgttgt
      301 taaatacctt atatgatctc tactgcccgc aggcaggcac cggtaaagga agccataaca
      361 acgaataata tgatcctgct ttccccgtaa accgttttat ccgtgcatca ttaaccctgt
      421 tcaggctgtg aaatgccaga ccggaacctg cgatacctta acgggaaaac aacagtggtc
      481 cgga
//
LOCUS       DAPHBJ010000464          484 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.467, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000464 DAPHBJ010000000
VERSION     DAPHBJ010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.467"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(47..439)
                     /locus_tag="RHZ76_004691"
     CDS             complement(47..439)
                     /locus_tag="RHZ76_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815164.1"
                     /translation="MKYILTVLCIFFCQTASAYPYRIYTAPEGAIVKNILTNEVIGKT
                     PVEVDVSNTEAGSTFGISMYRHENVAIKIFTVMPNAENNFTVSGPDVATMSLPGKAPL
                     NVTNDSNAAAVHIDLRPFLSEPAHHPVQ"
BASE COUNT          148 a           91 c          101 g          144 t
ORIGIN      
        1 tatacggtat tgttaagcag gccccaaaga ttggggccta gaagtgttat tgaacagggt
       61 gatgggccgg ttcagacaga aaaggtctta gatctatatg aaccgctgct gcattactgt
      121 cattggtcac gtttaacggg gccttacccg gtaaagacat agtggcaacg tctggaccag
      181 aaacagtaaa attattttct gcatttggca ttactgtgaa aattttaata gctacattct
      241 catgacgata cattgaaatt ccgaaagttg atccagcttc agtattgctg acatcaactt
      301 caaccggtgt tttcccgatc acttcattag taagtatatt tttcacaatt gcaccttctg
      361 gggcagtgta aatgcgataa gggtaagcac ttgcagtctg gcagaaaaaa atacacaaaa
      421 cggtcaatat atatttcatg attgctcctt ttgcaaaaat tctaaatcaa atgattgagt
      481 gact
//
LOCUS       DAPHBJ010000465          471 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.468, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000465 DAPHBJ010000000
VERSION     DAPHBJ010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.468"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>471)
                     /locus_tag="RHZ76_004692"
     CDS             complement(<1..>471)
                     /locus_tag="RHZ76_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HDV2815165.1"
                     /translation="MDKDAQGYIDLSDLDLTSCHFKGDVISKVSFLSSNLQHVTFECK
                     EIGDCNFTTAIVDNVIFRCRRLHNVIFIKASGECVDFSKNILDTVDFSQSQLGHSNFR
                     ECQIRNSNFDNCYLYASHFTRAEFLSAKEISFIKSNLTAVMFDYVRMSTGNFKD"
BASE COUNT          164 a           95 c           68 g          144 t
ORIGIN      
        1 caatctttaa aattccctgt cgacattcgc acataatcaa acataacagc tgtcaaattc
       61 gatttaataa atgatatttc tttggcagac agaaactctg ctctggtgaa gtgcgaagcg
      121 taaagataac aattatcgaa gtttgaattt ctaatctgac attcgcgaaa attactatga
      181 ccaagttgac tctgcgagaa gtcaactgta tcaagaatat ttttgctgaa atcgacacat
      241 tcaccactcg ctttgataaa aatcacattg tgtaaacgtc gacatctaaa tatgacatta
      301 tcaactattg cagtagtaaa attgcaatcc ccaatttctt tacattcgaa tgttacatgt
      361 tgtagatttg atgataaaaa agacaccttc gatataacgt cacctttaaa atgacaactt
      421 gttaaatcca aatcagacag gtcgatatac ccctgcgcat ctttatccat c
//
LOCUS       DAPHBJ010000466          468 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.469, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000466 DAPHBJ010000000
VERSION     DAPHBJ010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.469"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..459
                     /locus_tag="RHZ76_004693"
     CDS             <1..459
                     /locus_tag="RHZ76_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2815166.1"
                     /translation="YAIEDEIRGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKK
                     SRLGEAFSYVLNQWDALCYYSDDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGER
                     GALLYGLIGTCRLNGIDPEAYLRHILSVLPEWPSNRVDELLPWNVVLTNK"
BASE COUNT          119 a          105 c          140 g          104 t
ORIGIN      
        1 tacgccatcg aggatgaaat acggggatta ccggagtcag agcgtcttgc cgtcaggcag
       61 cagcgaagca aagtgttact gacgtcgctg catgaatgga tggtggagaa gaatggtacg
      121 ctgtcgaaaa aatccagact gggcgaagcg ttcagctatg tactgaatca gtgggatgcc
      181 ctctgttatt acagtgatga cggtctggcg gaggcggata ataatgctgc ggaaagagcg
      241 cttcgtgcag tctgtctcgg aaagaaaaac tttatgttct ttggcagcga tcacggcggc
      301 gagcgtggag cactgttgta cgggctgatc ggcacctgcc gtctgaacgg tatcgatccg
      361 gaagcgtatc tgcgccatat cctgagcgta ctgccggaat ggccttccaa ccgagttgat
      421 gaactcctgc catggaacgt agtactcacc aataaataag cgtcaata
//
LOCUS       DAPHBJ010000467          455 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.470, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000467 DAPHBJ010000000
VERSION     DAPHBJ010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.470"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>455)
                     /locus_tag="RHZ76_004694"
     CDS             complement(<1..>455)
                     /locus_tag="RHZ76_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815167.1"
                     /translation="KVYAYIHEILSKEYGVFSLKEFAKNDFDALVDFFLKERNTEKCL
                     DFIEICFQILVSHVAKNHYEFKDITSQSPGDAVIELNERFREHGVGYQFESEEIIRID
                     SQLIHADVVKPTLILLSGEPLFEGANDEFLAAHEHYRHKRYKECLNDCL"
BASE COUNT          136 a           96 c           71 g          152 t
ORIGIN      
        1 cagacaatca tttagacatt ctttataacg cttatgcctg taatgctcat gcgcagcaag
       61 gaattcatca ttggcgcctt caaacaaggg ttctccactt aaaagaatca gggttggctt
      121 tacgacatca gcatgaatca attgagagtc tatccttatt atctcttcag attcaaactg
      181 ataaccaacc ccatgttctc taaacctttc atttagttca ataactgcat ctccaggact
      241 ttgacttgtt atgtctttga attcataatg gtttttcgca acgtggctca ctagtatctg
      301 gaagcatatt tctatgaaat ctaggcattt ctctgtattt ctctctttaa ggaaaaagtc
      361 gacaagagcg tcaaagtcat tctttgcaaa ttccttcaaa gaaaaaacac catattcctt
      421 ggaaagtatc tcatggatat atgcatatac tttgt
//
LOCUS       DAPHBJ010000468          453 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.471, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000468 DAPHBJ010000000
VERSION     DAPHBJ010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.471"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>453
                     /locus_tag="RHZ76_004695"
     CDS             <1..>453
                     /locus_tag="RHZ76_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815168.1"
                     /translation="KISRNLIHTVTKFDENEKSKPTDLFTWHGKDKKYNTLAIVIKNI
                     NGNDYLSLGYYDQDNYHIKRGIRINGDSLTQYCSENARNASASFESNKALMAELFATG
                     INHQVVNELNGERLKEPNEVFKRYGRAIRYNFQVDNAKYRRNNVKEIVS"
BASE COUNT          185 a           77 c           80 g          111 t
ORIGIN      
        1 aaaatatcac gtaacctcat acatactgta acgaaattcg atgagaacga gaaaagcaaa
       61 ccaacggact tatttacctg gcacggtaaa gataaaaaat acaacactct cgccattgta
      121 attaaaaata taaacggaaa cgattattta tcgctcggtt actacgatca ggacaactac
      181 cacattaaaa gaggaattcg tattaatggt gatagtctca cacaatattg tagtgaaaac
      241 gccaggaatg cttcagcgtc atttgaaagc aataaagctc tcatggcaga attatttgca
      301 acaggtataa atcatcaagt tgtaaacgaa ctcaacgggg aaagactgaa agaaccaaac
      361 gaagttttta aacgttatgg tcgagcaata agatataatt ttcaagtgga caatgcaaaa
      421 tatagacgca ataatgtaaa agaaatagtt tct
//
LOCUS       DAPHBJ010000469          451 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.472, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000469 DAPHBJ010000000
VERSION     DAPHBJ010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.472"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>451
                     /locus_tag="RHZ76_004696"
     CDS             <1..>451
                     /locus_tag="RHZ76_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2815169.1"
                     /translation="ADDNAITDGSVTFNGKVIAPACTLVAATKDSVVTLPDVSATKLQ
                     TNGQVSGVQTDVPIELKDCDTTVTKNATFTFNGTADTTQITAFANQASSDAATNVALQ
                     MYMNDGTTAITPDTETGNILLQDGDQTLTFKVDYIATGKATSGNVNAV"
BASE COUNT          130 a           92 c          109 g          120 t
ORIGIN      
        1 ctgctgatga taatgctatc acggatggct cagtaacatt taatggtaaa gttattgctc
       61 cagcttgtac cctggtagct gcgacgaaag attccgtggt gactttgcca gatgttagtg
      121 ccacgaagtt gcaaaccaat ggtcaggttt ctggcgtgca aactgatgtg ccaattgaat
      181 taaaagattg tgatactacc gtaacaaaaa atgcaacgtt cacctttaat ggcactgcgg
      241 atactactca gattacagcg tttgctaacc aggcctcatc tgatgctgct acaaacgtgg
      301 ccctgcaaat gtatatgaat gatggtacaa cggccatcac gccagacaca gaaaccggga
      361 acattttgtt gcaggatgga gatcagacgt tgacttttaa agttgattat atcgctacgg
      421 ggaaagcgac ttcaggtaat gtgaatgcgg t
//
LOCUS       DAPHBJ010000470          445 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.473, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000470 DAPHBJ010000000
VERSION     DAPHBJ010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.473"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT          152 a           68 c           84 g          141 t
ORIGIN      
        1 ttgacgaata gtttcatgct tgagaaatta caaatggaaa gagatgtaat tgaaaagaaa
       61 tctcgggagg aacttgttaa ggaattacaa gaagaaagcg ttgatgtcta aaattaaaaa
      121 agcgatacta cgcttgaaga ggtttctttc ttgctaatgt atttttcatg aaaaaaaggg
      181 ttacattgaa atgtaaccct aacctattga attgatgcac ctcacattca ctactcatag
      241 ttttccttcc gatattttga gatctattat ttctttggct tgagatccat tgtgcaggtc
      301 accgtaactt aaaatttcga gtaatcaggg agtttcatct ttggtattaa ttagaccgtc
      361 agacgcaaat gtaacgtttc tgaattaatc gtaaatgtaa attagcaaaa aaaatttcta
      421 gtgcagagct cctccaaaac ggagg
//
LOCUS       DAPHBJ010000471          435 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.474, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000471 DAPHBJ010000000
VERSION     DAPHBJ010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.474"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>435
                     /locus_tag="RHZ76_004697"
     CDS             <1..>435
                     /locus_tag="RHZ76_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2815170.1"
                     /translation="ALRQKDQQLSLVEETEAFLRSALTRAEEKIEEDEREIEHLRAQI
                     EKLRRMLFGTRSEKLRREVELAEALLKQREQDSDRYSGREDDPQVPRQLRQSRHRRPL
                     PAHLPREIHRLEPEESCCPECGGELDYLGEVSAEQLELVSSAL"
BASE COUNT          110 a          112 c          133 g           80 t
ORIGIN      
        1 gccctccgtc agaaagacca gcaactgagt ctggttgaag agacggaagc cttcctgcgc
       61 tctgcactga cacgtgccga agaaaagatc gaagaagatg aacgggaaat agaacatctg
      121 cgggctcaga tagaaaaact gcgccggatg ctgttcggta cccgttctga aaaactgcgt
      181 cgtgaagttg aactggctga ggctctgctg aaacaacgtg aacaggacag cgatcgttac
      241 agtgggcggg aagacgatcc tcaggttccc cgccagttgc gacagtcgcg ccatcgtcgt
      301 ccgttaccgg cacaccttcc ccgtgaaata caccgcctgg agccagaaga aagctgttgc
      361 ccggagtgtg gcggtgagct ggattatctg ggggaagtca gcgctgaaca gctggaactg
      421 gtgagcagtg ccctg
//
LOCUS       DAPHBJ010000472          434 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.475, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000472 DAPHBJ010000000
VERSION     DAPHBJ010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.475"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>434
                     /locus_tag="RHZ76_004698"
     CDS             <1..>434
                     /locus_tag="RHZ76_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815171.1"
                     /translation="TDNAKTPDTPKWPCDNWEEQQPINSTFSGEGYISDQYDLAQHQL
                     QQINACHTNTTYTNADYSKVVAQLVSLITNIETISSTQLTQQTQSILNQINNIRYEKN
                     KSAECRIIVVANPKPDKAIITKISVEEGIPITFSVQTMFSDT"
BASE COUNT          170 a           94 c           66 g          104 t
ORIGIN      
        1 accgataatg ctaaaacgcc agacacaccg aagtggcctt gcgataattg ggaagaacaa
       61 caacccatta attccacatt ctctggagaa ggatatatct ctgatcaata tgatttagcg
      121 caacaccaac ttcaacaaat taatgcgtgc cacacgaaca ctacatatac aaatgcagac
      181 tactcaaaag ttgtggctca acttgtaagt cttattacta acattgaaac gataagttcg
      241 acacaactta cgcaacagac acaatcaata cttaatcaga ttaataacat tagatatgaa
      301 aaaaacaaaa gtgctgaatg tcgaatcatt gttgtcgcta atcctaaacc cgacaaggca
      361 atcataacta aaattagcgt agaagaggga atacccataa cattcagtgt ccaaaccatg
      421 ttttcagata ctaa
//
LOCUS       DAPHBJ010000473          428 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.476, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000473 DAPHBJ010000000
VERSION     DAPHBJ010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.476"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>428
                     /gene="tuf"
                     /locus_tag="RHZ76_004699"
     CDS             <1..>428
                     /gene="tuf"
                     /locus_tag="RHZ76_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDV2815172.1"
                     /translation="PIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFL
                     LPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGR
                     AGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVY"
BASE COUNT          104 a           98 c          125 g          101 t
ORIGIN      
        1 ccgatcgttc gtggttctgc tctgaaagcg ctggaaggcg acgcagagtg ggaagcgaaa
       61 atcctggaac tggctggctt cctggattct tacattccgg aaccagagcg tgcgattgac
      121 aagccgttcc tgctgccgat cgaagacgta ttctccatct ccggtcgtgg taccgttgtt
      181 accggtcgtg tagaacgcgg tatcatcaaa gttggtgaag aagttgaaat cgttggtatc
      241 aaagagactc agaagtctac ctgtactggc gttgaaatgt tccgcaaact gctggacgaa
      301 ggccgtgctg gtgagaacgt aggtgttctg ctgcgtggta tcaaacgtga agaaatcgaa
      361 cgtggtcagg tactggctaa gccgggcacc atcaagccgc acaccaagtt cgaatctgaa
      421 gtgtacat
//
LOCUS       DAPHBJ010000474          427 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.477, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000474 DAPHBJ010000000
VERSION     DAPHBJ010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.477"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            30..>427
                     /locus_tag="RHZ76_004700"
     CDS             30..>427
                     /locus_tag="RHZ76_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HDV2815173.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFPHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDG"
BASE COUNT          135 a           84 c           89 g          119 t
ORIGIN      
        1 catatacaat gactctggag gaaactgttt tggcaattcg tcttcacaaa ctagctgttg
       61 ctttaggtgt ctttattgtt agcgctcctg cctttccgca tggtcatcac tcacacggca
      121 aacccttaac agaggtcgaa caaaaagctg ctaatggtgt ttttgatgat gccaatgtac
      181 aaaaccgaac gctcagtgac tgggatggag tctggcaatc cgtttatcct ttactgcaaa
      241 gtggcaaact tgaccccgtc tttcagaaaa aagcggatgc agataaaacc aaaacatttg
      301 ctgaaattaa agattattat cacaaaggtt atgcgacaga tatcgagatg attggcattg
      361 aggacggcat tgttgaattc catagaaata atgaaacaac atcctgtaaa tatgattacg
      421 atggata
//
LOCUS       DAPHBJ010000475          414 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.478, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000475 DAPHBJ010000000
VERSION     DAPHBJ010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.478"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            88..279
                     /locus_tag="RHZ76_004701"
     CDS             88..279
                     /locus_tag="RHZ76_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HDV2815174.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          102 a          106 c           80 g          126 t
ORIGIN      
        1 tacataacga agaattgacc attttgtcct gttgtgcctt aatgtaagta ccgtccacag
       61 cgtgggacat acttcaagga accttttgtg agtcaggcaa ccagtatgcg aaaacgacac
      121 cgatttaaca gtcgcatgac ccgtatcgta ctgctcatca gctttatctt cttctttggc
      181 cgttttatct actcgtccgt cggtgcctgg cagcaccatc agagcaaaaa agaagctcag
      241 caatccacac tctccgtcga atcaccggta caacgttagc ggttaccttc tccactttca
      301 cagaacataa cggcacttcg ctgtcggatg cttttgctgt ttgggattat caaagcggca
      361 gatattcttt catcttaaat tttacgtctt tatcctgact gatgtttatc ctgt
//
LOCUS       DAPHBJ010000476          412 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.479, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000476 DAPHBJ010000000
VERSION     DAPHBJ010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.479"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            48..>412
                     /gene="lacY"
                     /locus_tag="RHZ76_004702"
     CDS             48..>412
                     /gene="lacY"
                     /locus_tag="RHZ76_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HDV2815175.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGA"
BASE COUNT           87 a           87 c           81 g          157 t
ORIGIN      
        1 aaccgggcag gccatgtctg cccgtatttc gcgtaaggaa atccattatg tactatttaa
       61 aaaacacaaa cttttggatg ttcggtttat tctttttctt ttactttttt atcatgggag
      121 cctacttccc gtttttcccg atttggctac atgacatcaa ccatatcagc aaaagtgata
      181 cgggtattat ttttgccgct atttctctgt tctcgctatt attccaaccg ctgtttggtc
      241 tgctttctga caaactcggg ctgcgcaaat acctgctgtg gattattacc ggcatgttag
      301 tgatgtttgc gccgttcttt atttttatct tcgggccact gttacaatac aacattttag
      361 taggatcgat tgttggtggt atttatctag gcttttgttt taacgccggt gc
//
LOCUS       DAPHBJ010000477          405 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.480, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000477 DAPHBJ010000000
VERSION     DAPHBJ010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.480"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(149..367)
                     /locus_tag="RHZ76_004703"
     CDS             complement(149..367)
                     /locus_tag="RHZ76_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815176.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
BASE COUNT          139 a           83 c           73 g          110 t
ORIGIN      
        1 atagaaatta tttcaatgat atataaaaac aactgtgata acaccagggt tgacaactat
       61 ttgcgcaata attcctttaa ttcaatgatc caggcattca ctgccagcaa cccattcagc
      121 cgcatactga cagtgagaaa ctgaaagatt attgctgatc cctgtggctg acgagtcttt
      181 cccttaatcg attaaccaga ttgatcgcca gccaaacaag ggcattaagc aaatttatcg
      241 tcacaagcac ttctcccagc ccggtactgg gttttgcaat aagaatgacc aggagggtga
      301 tcagcataaa tgcaatggaa aagatgatat agagagtgaa ataatccaga ttattactat
      361 tgttcatgct actaatggca atcctgtcaa aacttttaaa caatt
//
LOCUS       DAPHBJ010000478          404 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.481, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000478 DAPHBJ010000000
VERSION     DAPHBJ010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.481"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(19..375)
                     /locus_tag="RHZ76_004704"
     CDS             complement(19..375)
                     /locus_tag="RHZ76_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDV2815177.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
BASE COUNT          150 a           89 c           86 g           79 t
ORIGIN      
        1 atcgccccaa ttctaagatt aatttgaacc aaacttcgga tcgtttccgt aacgattgct
       61 gccagaagta ccttctgtgc agaaaaaaac cagaagcacg agccagccaa taaacggaac
      121 aaaaaagagc aaggcccagg cacctgaacg gtcggtatcg tgtaagcgac ggatagcaag
      181 cgcaaggacg ggaaggaagg ttgccagcaa ataaagcata gacagatatg gaagctccag
      241 acctaaaatc aattgaatga cattgataat agcgccgacg atggcgttaa tcagagtaaa
      301 catccagtac tctttacgac gtgcgcgacc agagaaacca acataatttt ttaatacgga
      361 caaataccac tgcataaaaa catcccttta attaaaagtg atga
//
LOCUS       DAPHBJ010000479          392 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.483, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000479 DAPHBJ010000000
VERSION     DAPHBJ010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.483"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>392
                     /locus_tag="RHZ76_004705"
     CDS             <1..>392
                     /locus_tag="RHZ76_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="required for invasion of epithelial cells; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HDV2815178.1"
                     /translation="LCGCKQQELLNHLDQQQANDVLAVLQRHNINAEKKDQGKTGFSI
                     YVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKARLYSAIEQWLEQS
                     LKIMDGIISSRVHVSYDVDNGDSGKTALP"
BASE COUNT          118 a           74 c           85 g          115 t
ORIGIN      
        1 ttgtgcggct gcaagcagca ggaattattg aaccatcttg atcaacagca agctaatgat
       61 gtccttgctg ttttacaaag acataatatt aatgctgaaa agaaggatca aggcaaaact
      121 ggattctcaa tctatgttga accaacggat tttgcttctg cggtggattg gctaaaaatc
      181 tacaatctcc cgggtaagcc tgatattcaa atatcgcaga tgttccctgc agatgcactc
      241 gtttcatctc cacgagcgga aaaagcaagg ctatattctg ctattgagca gtggttagag
      301 caatctctca aaattatgga tggcattatt tcttcgagag tacatgttag ttacgatgtt
      361 gataatggcg atagtggtaa aactgcttta cc
//
LOCUS       DAPHBJ010000480          390 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.484, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000480 DAPHBJ010000000
VERSION     DAPHBJ010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.484"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            18..>390
                     /locus_tag="RHZ76_004706"
     CDS             18..>390
                     /locus_tag="RHZ76_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HDV2815179.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGRSDAFSELPENTIVVPYGELTGSFEIIITTDPDIVVTIRELTAQEPEDRTLTFN
                     QQVEVLGLKFAVKEKNEVWQYS"
BASE COUNT          132 a           55 c           83 g          120 t
ORIGIN      
        1 attagcgggg ggtattcatg gaaaataatg ataaattctt atcacaagac ttattggaat
       61 cttatgccat tcgtttgttg agcggaccat tgaatggatg cgagtatgaa atacttaatg
      121 ggcgtctact tgttatcatt ggtaatgatg tttcgttagg aagaagtgat gctttttctg
      181 agttaccaga aaacacaata gtcgttcctt atggcgagct tacaggtagt tttgagataa
      241 taattactac cgaccctgat atagtagtaa caatcagaga attaacagca caagaacctg
      301 aagatagaac gttaacattc aatcagcaag tagaagtttt aggtctcaaa tttgctgtaa
      361 aagaaaaaaa tgaagtttgg cagtattcat
//
LOCUS       DAPHBJ010000481          386 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.485, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000481 DAPHBJ010000000
VERSION     DAPHBJ010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.485"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(148..>386)
                     /locus_tag="RHZ76_004707"
     CDS             complement(148..>386)
                     /locus_tag="RHZ76_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HDV2815180.1"
                     /translation="EDEYSAEQVADCYRLRWQIELAFKRLKSLLHLDALRAKEPELAK
                     AWIFANLLAAFLIDDIIQPSLDFPPRSAGSEKKN"
BASE COUNT           91 a           98 c           89 g          108 t
ORIGIN      
        1 accatctgaa gaatgcgacg cctcggtgcc tcgttaagac gatgcctcgc gttcttcaat
       61 tgcgttttgt aggctgtcag ggatactgtc ccacgaatgg ccacctgtaa gctccagatg
      121 accatttttg ttattctcca caacgagtta gttcttcttt tcggatccgg cacttctggg
      181 ggggaaatcc agcgatggct ggattatgtc gtcaattaaa aatgcggcga gtagattagc
      241 aaatatccac gctttcgcga gttcaggttc ctttgcacgc aaagcatcca ggtgcagcaa
      301 acttttgagc cgcttaaaag ccagttcaat ttgccatcgc agacggtaac aatcagccac
      361 ttgctctgct gaatattcat cttccg
//
LOCUS       DAPHBJ010000482          382 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.486, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000482 DAPHBJ010000000
VERSION     DAPHBJ010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.486"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(285..>382)
                     /locus_tag="RHZ76_004708"
     CDS             complement(285..>382)
                     /locus_tag="RHZ76_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDV2815181.1"
                     /translation="AVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
BASE COUNT          107 a           90 c           66 g          119 t
ORIGIN      
        1 ctggaatact aagcacatca taaaaattat catcacatgg tatatctgaa acacacgaaa
       61 gcatacctaa agttatgctg tataaacagg tggctaatcc caaggggatc gattccagaa
      121 atcgggttca acggatactg ataaaaattc cacctacctt ccagcccaat cggagcctga
      181 gtgttatacc gcccctgctg cggttaatgt taccgataat agttatagta cagccctgat
      241 tcatcatcat actactgctg cggcagtctg atgaactatt gcttttacgg attctcctta
      301 ttcttccttc tatccattcg ctttagcttt ttgagctctt gaagtttgct ctttgcatca
      361 atattaccgt tatttacagc ga
//
LOCUS       DAPHBJ010000483          372 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.487, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000483 DAPHBJ010000000
VERSION     DAPHBJ010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.487"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT           78 a          109 c          122 g           63 t
ORIGIN      
        1 gaaaattacc tgaactgcgt caactcggag tctggccgcg tgcgcgtgga agcaaagcgg
       61 gcgagcgaag aatacgggcg agtgctgcgg cgctatccgc tgaaacccgg cgagacggtc
      121 gagcgtccgc aacgcgggga agtcgtcagc agctccgggg ttctcccccc actggaccca
      181 ggcgaatact aagcgcggcg gtgtgtaata gacggagcgc gaggcgacta ctggtcaggg
      241 tttgcgcaag gcgggtgtgt aatagacggg aggggatcga tccggcgatc cagcctgtgg
      301 atagtccgtc tattacccac ccggttccgt cgtttaccca cccaaccccg tctattaccc
      361 accggaaacc gt
//
LOCUS       DAPHBJ010000484          369 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.488, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000484 DAPHBJ010000000
VERSION     DAPHBJ010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.488"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>369
                     /locus_tag="RHZ76_004709"
     CDS             <1..>369
                     /locus_tag="RHZ76_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815182.1"
                     /translation="CNEESSVFHRKELSNLKIQWVDFPRTAAKQKESLIGLMSRKFSH
                     IADSKAAIEVILALFRNVETVYNQGQEICLLDPTKRVEGEEVKKVMNIIDSQQKAFDY
                     WRDEAQQFSMKFRIPLSIQKN"
BASE COUNT          143 a           60 c           70 g           96 t
ORIGIN      
        1 tgtaatgaag aatcttctgt tttccatcgt aaagaactaa gtaacctaaa aattcagtgg
       61 gtagattttc caagaacagc tgcaaaacaa aaagaatcgc ttattggact tatgtcaaga
      121 aagttttctc atatagctga ctcgaaagca gccatcgagg taatacttgc tctatttaga
      181 aatgtggaaa ctgtatataa tcaaggacag gaaatatgtc tactcgatcc aacaaaaagg
      241 gttgaaggcg aagaagttaa gaaagttatg aatataattg attcacagca aaaggcattc
      301 gactattggc gtgacgaagc acagcaattc tcaatgaaat tcagaatacc acttagcata
      361 cagaaaaat
//
LOCUS       DAPHBJ010000485          366 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.489, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000485 DAPHBJ010000000
VERSION     DAPHBJ010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.489"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT          116 a           77 c           60 g          113 t
ORIGIN      
        1 aaaatctctg ctgatgttta attgatttga attttcgtaa gctatatcgc ttactcaatc
       61 cattttaccc agagtcataa aatagagtgt ctgttgatct cattttcata ttcctttcag
      121 actgaagtgc gttttcatca cccgatgaga aagaactgaa gtgatgtttg ccatagatta
      181 cgagatgccc agtctcattg actatcgcgg tgcaccttta tacctgttat accagatcaa
      241 aaatcacgca atccatacaa caaaaccaga tttgcaattc gtgtcacaaa atatgtcgat
      301 cttttcctaa gaagcagatg ccatgtgaag acagacgaac acttgcattg gtcttcaaaa
      361 actaaa
//
LOCUS       DAPHBJ010000486          365 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.490, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000486 DAPHBJ010000000
VERSION     DAPHBJ010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.490"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>365
                     /locus_tag="RHZ76_004710"
     CDS             <1..>365
                     /locus_tag="RHZ76_004710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815183.1"
                     /translation="LDWLIKMVNIKNAIPGRKFNIANFDNGNTWSALLHERMNAINNM
                     SQMIDERDRTIHDQKCLIDERDRTIHDQKRLIDERDSTVLTQKNLIDERDLVSAQQNQ
                     LIEQNNKTIQQLIQNVTDL"
BASE COUNT          136 a           66 c           67 g           96 t
ORIGIN      
        1 tcttgattgg cttataaaga tggtcaacat taaaaacgcc ataccaggta gaaaattcaa
       61 cattgcaaat tttgacaacg gtaacacctg gtcggctttg cttcatgaac gcatgaatgc
      121 aataaataat atgtcgcaaa tgatagatga gcgtgacaga acaattcatg atcaaaaatg
      181 cttaattgat gaacgcgata gaactattca cgaccagaag cgccttattg atgagcgaga
      241 cagcactgtt cttacgcaaa aaaatttaat tgatgagcgt gacctggtct ctgctcagca
      301 aaatcaatta attgagcaga ataataaaac tattcagcag ctaattcaaa atgtaactga
      361 cttaa
//
LOCUS       DAPHBJ010000487          364 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.491, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000487 DAPHBJ010000000
VERSION     DAPHBJ010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.491"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT           85 a           99 c           68 g          112 t
ORIGIN      
        1 tcctgtgatc gccaaaatgc ctctcctgac gttctccaac cccttgtaat actgtcaatc
       61 cagatatttt ctactgctca atattcaatc ggcttcagga atacctggtc taaggttttg
      121 tgatggacga tgagcggctg aagaatccgc ctgtcggttc ttctgtcctc gaacgcatcc
      181 gcgatattca cgccagtatg cgccgtgttt atttgcaggt gatttgcccc cctgctgact
      241 ctcagccaac tgtatcgcca ttaaggtgaa gatgttgttt tactcgcccc agaacaccct
      301 tttttatacg actattctat ttctttaacc gctggcgaaa aaaactgact ataaacttca
      361 ccaa
//
LOCUS       DAPHBJ010000488          355 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.492, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000488 DAPHBJ010000000
VERSION     DAPHBJ010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.492"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(18..170)
                     /gene="mokC"
                     /locus_tag="RHZ76_004711"
     CDS             complement(18..170)
                     /gene="mokC"
                     /locus_tag="RHZ76_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HDV2815184.1"
                     /translation="MKQHKAMIVALIVICITAVVAVLVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT           76 a          104 c           77 g           98 t
ORIGIN      
        1 actccccgcc gttgctctta ctcggattcg taagccgtga aaacagcaac ctccgtctgg
       61 ccagttcgga tgtgaacctc acagaggtct tttctcgtta ccagcaccgc cactacggcg
      121 gtgatacaga tgacgatcag ggcgacaatc atcgccttat gctgcttcat tgctctcttc
      181 tccttgacct tacggtcagt aagaggcact ctacatgtgt tcagcatata gggggcctcg
      241 ggttgatagt aaaatatcac tcggggcttt tctctatctg ccgttcagct aatgcctgag
      301 acagatagcc tcaagcaccc gccgctatta tatcgctctc tttaacccat tctgt
//
LOCUS       DAPHBJ010000489          347 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.denovo.494, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000489 DAPHBJ010000000
VERSION     DAPHBJ010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.denovo.494"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>347
                     /locus_tag="RHZ76_004712"
     CDS             <1..>347
                     /locus_tag="RHZ76_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2815185.1"
                     /translation="DCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPLYRQSEIF
                     ARQGVELSRALLSNWVDACCQLMTPVNDALYRYVMNTRKVHTDDTPVKVLAPGQKKAK
                     TGRIWTYVRDDRNV"
BASE COUNT           82 a           91 c          100 g           74 t
ORIGIN      
        1 gactgtattg ttgaagcacc ggcgccgtcc cgcccgatag agcgtggtat cgcgggcccc
       61 ggattacttg cccgcgtgtt aacgggaaaa tactgcgaac atctgccact gtatcgtcag
      121 agtgaaatct ttgcccgcca gggtgtcgaa ctgagccggg ccttactctc caactgggtt
      181 gacgcgtgct gccagttaat gacaccggtg aatgatgccc tgtaccgtta tgtaatgaac
      241 acccgcaagg ttcacactga tgacacaccg gtaaaggtac tggcaccggg tcagaaaaag
      301 gcgaaaacag ggcgtatctg gacgtatgtc cgggatgatc gcaatgt
//
LOCUS       DAPHBJ010000490        18040 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.guided.087, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000490 DAPHBJ010000000
VERSION     DAPHBJ010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.guided.087"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="RHZ76_004713"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="RHZ76_004713"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDV2815186.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="RHZ76_004714"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="RHZ76_004714"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDV2815187.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDISDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="psd"
                     /locus_tag="RHZ76_004715"
     CDS             2040..3008
                     /gene="psd"
                     /locus_tag="RHZ76_004715"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDV2815188.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            3030..6353
                     /gene="mscM"
                     /locus_tag="RHZ76_004716"
     CDS             3030..6353
                     /gene="mscM"
                     /locus_tag="RHZ76_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HDV2815189.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6382..6696)
                     /locus_tag="RHZ76_004717"
     CDS             complement(6382..6696)
                     /locus_tag="RHZ76_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HDV2815190.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(6693..7007)
                     /locus_tag="RHZ76_004718"
     CDS             complement(6693..7007)
                     /locus_tag="RHZ76_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2815191.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            complement(7059..8561)
                     /gene="yjeM"
                     /locus_tag="RHZ76_004719"
     CDS             complement(7059..8561)
                     /gene="yjeM"
                     /locus_tag="RHZ76_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HDV2815192.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGMTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHLSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            complement(8780..9757)
                     /gene="epmA"
                     /locus_tag="RHZ76_004720"
     CDS             complement(8780..9757)
                     /gene="epmA"
                     /locus_tag="RHZ76_004720"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDV2815193.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            10082..11890
                     /gene="frdA"
                     /locus_tag="RHZ76_004721"
     CDS             10082..11890
                     /gene="frdA"
                     /locus_tag="RHZ76_004721"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDV2815194.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11883..12617
                     /gene="frdB"
                     /locus_tag="RHZ76_004722"
     CDS             11883..12617
                     /gene="frdB"
                     /locus_tag="RHZ76_004722"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDV2815195.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12628..13023
                     /gene="frdC"
                     /locus_tag="RHZ76_004723"
     CDS             12628..13023
                     /gene="frdC"
                     /locus_tag="RHZ76_004723"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDV2815196.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            13034..13393
                     /gene="frdD"
                     /locus_tag="RHZ76_004724"
     CDS             13034..13393
                     /gene="frdD"
                     /locus_tag="RHZ76_004724"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDV2815197.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            13456..14589
                     /locus_tag="RHZ76_004725"
     CDS             13456..14589
                     /locus_tag="RHZ76_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDV2815198.1"
                     /translation="MFKTTLCALLITASCFTFAAPQKINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWIRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVTAAWQILNALQ"
     gene            14678..15211
                     /gene="blc"
                     /locus_tag="RHZ76_004726"
     CDS             14678..15211
                     /gene="blc"
                     /locus_tag="RHZ76_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDV2815199.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(15208..15525)
                     /gene="sugE"
                     /locus_tag="RHZ76_004727"
     CDS             complement(15208..15525)
                     /gene="sugE"
                     /locus_tag="RHZ76_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDV2815200.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15707..15853)
                     /gene="ecnB"
                     /locus_tag="RHZ76_004728"
     CDS             complement(15707..15853)
                     /gene="ecnB"
                     /locus_tag="RHZ76_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDV2815201.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15964..16089)
                     /gene="ecnA"
                     /locus_tag="RHZ76_004729"
     CDS             complement(15964..16089)
                     /gene="ecnA"
                     /locus_tag="RHZ76_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDV2815202.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(16141..16707)
                     /gene="efp"
                     /locus_tag="RHZ76_004730"
     CDS             complement(16141..16707)
                     /gene="efp"
                     /locus_tag="RHZ76_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDV2815203.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16749..17777
                     /gene="epmB"
                     /locus_tag="RHZ76_004731"
     CDS             16749..17777
                     /gene="epmB"
                     /locus_tag="RHZ76_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDV2815204.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         4511 a         4752 c         4634 g         4143 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc caaaaaagtt ttttttgcgt ttttatctca agtgctgaaa ttatcagcaa
      181 gacgttcaat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccagctcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaaaccatcc agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctgacatc gagataacgg tagtggaagt
      421 aggcttccag ctccggcatg tatttaaaca ggaaacgacg gtcctgaccg atgctgttac
      481 cgcaaatcgg cgattttccc gcaggcaccc actgttttaa aaattcgagc gttgccagtt
      541 cagcttcccg atcgcccatc gtgctcgctt tcacgcgctc taccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcagactgg tgtactgcaa
      661 tggtcggccc ttctgccaga atattcaggt tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtctgtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgctgg taaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccggc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgta
     1201 cctcggtgtt gacgcgtccg gatttctacg acggcgtaaa acctattgcc gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat tgtgctcaac aagatagacc
     1381 tgctggacga cgaaggcatg gcgttcgtca acgagcagat ggatatctac cgcaatatcg
     1441 gttatcgcgt attgatggtt tccagccata ctcaggatgg gctaaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgtcggcaaa tccagcctgc
     1561 tgaatgcgtt gctggggctg caaaaagaga tcctgaccaa cgatatctct gacaactcgg
     1621 ggctcggtca gcacaccacg accgccgctc ggctgtatca cttcccgcac ggcggtgatg
     1681 tgattgactc cccaggagtg cgtgagttcg gcctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaagggaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgtagg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca atcgataccg
     2281 atccgaatgt actggtcatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg ctggcaggca
     2401 actatctgat ggcggacctg ttccgcaacg gtacgtttgt gaccacttac ctctccccgc
     2461 gtgactacca ccgcgtacac atgccgtgca acggtattct gcgtgagatg atctacgtgc
     2521 cgggcgatct cttctccgtt aaccatctca cggctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatttgc cttttcgata ccgaatttgg cccaatggcg cagattctgg
     2641 tcggagcgac gattgttggc agcattgaga cggtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggtat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcac
     2761 tgctgaaagg ccaggaaatg ggtcgcttta aactcggttc caccgttatc aacctgtttg
     2821 caccgggtaa agtgaatctg gttgagcaac tggaaagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga cgctgaacct gccccgcttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccagcccatt ggttgacgac aaaaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atcactcagg
     3121 aactggagca ggcaaaagcg gcgaaacccg cacagccgga agtcgtagag gcgctccagt
     3181 ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaaa caatatcagc
     3241 aagttatcga taattatccg aaactctccg ctactctgcg cgcacaatta aacaacatgc
     3301 gtgacgagcc gcgcagcgtg tcgccgggaa tgtctaccga cgcgctgaat caggaaattc
     3361 tccaggtcag cagccagttg ctggataaaa gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca gcaaaccgac gcccgccgtc
     3481 agttaaatga gatcgagcgc cgcctgggaa cgcttaccgg caatactccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactctg cacgtcttaa ggcgctcgtt gatgaactgg
     3601 agctggcgca gctgtctgcc aataaccgcc aggaattagc gcgcttacgc tcagagctgg
     3661 cggaaaaaga gagccagcaa ctggatgcgt atttgcaggc cttgcgtaat caattaaaca
     3721 gccaacgtca gcttgaggcg gagcgggcgc tggaaagtac cgaattgctg gcagaaaaca
     3781 gcgccgattt gccgaaagat atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcgg
     3841 ctttgaatca acaggcgcag cggatggatc tcgttgcctc gcaacagcgt caggctgcca
     3901 gccagacgtt acaggtccgg caggcgttga atacgctgcg tgaacagtcg caatggctgg
     3961 gatcgtccaa tctgctcggc gaagcgctgc gggcgcaggt ggcacggctg ccggaaatgc
     4021 cgaaaccaca acagcttgat accgaaatgg cgcagttgcg tgtgcaacgg ttacgttatg
     4081 aggatctgct taataaacag ccgctgctac ggcaaattca tcaggccgac ggtcagccgc
     4141 tgactgccga gcaaaaccgt attctggaag cacaactgcg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gacacgctac tattggaact gaccaagctg aaagtctcca
     4261 acgggcaact ggaggatgcg ctgaaagagg tgaacgaagc aacgcaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagcaaaga gacaattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtt ggcaaagtga
     4561 ctcaggatca cttctggctg acgttgcgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgttaccggt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggcgat ggtgtaacgg ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgccac ctttgcccgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgtgtttc ccgtgggatg cgctactacc tgatgagcat cgggcttatt gtgccgctga
     4861 ttatggcgct gatgatgttc gataacctcg acgaccgtga attctccggt tcgctgggac
     4921 ggctttgctt tatcctcatt tgcggtgcgc tggcggtggt caccctcagc ctgaaaaagg
     4981 ccgggattcc gctgtatctc aacaaagagg gcagcggcga caacattacc aaccatatgc
     5041 tgtggaacat gatgattggc gcgccgttgg ttgccattct ggcgtcggcg gtgggttatc
     5101 tggcaacggc acaggcgctg ttagcgaggc ttgaaacctc ggttgccatc tggttcctgc
     5161 tactggtggt ttatcacgtt atccgccgct ggatgctgat ccagcgccgc aggctggcgt
     5221 ttgatcgggc gaagcatcgc cgggcagaga tgttagcgca acgtgcgcgt ggcgaagagg
     5281 aagcgcatca tcacagtagc ccggaaggag caattgaagt cgatgaaagc gaagtcgatc
     5341 tcgatgccat cagtgcgcaa tccttgcggc tggtgcgctc aattttgatg ttgatcgccc
     5401 tgctttctgt cattgtgctg tggtcagaaa tccattccgc tttcggcttc ctcgaaaata
     5461 tttcgctgtg ggatgtcacc tccacggtac agggcgtaga aagtctggag ccaattaccc
     5521 tcggtgcggt gctgattgcc attctggtgt ttatcatcac cacgcagctg gtgcgcaact
     5581 tgcccgcgct gctggaactg gcgattttgc agcacctgga tttaacgccg ggtacgggtt
     5641 acgccatcac caccatcacc aaatatctgc tgatgctgat tggcgggctg gtcggcttct
     5701 caatgattgg tattgagtgg tcgaaattgc agtggctggt tgccgcgctc ggtgttggtc
     5761 tcggttttgg tttgcaggaa attttcgcca actttatctc tggtctgatt atcctgttcg
     5821 aaaaaccgat tcgcattggc gatacggtga caattcgcga tctcaccggt agcgtgacga
     5881 aaattaacac ccgcgccacc accatcagcg actgggaccg taaagagata atcgtgccga
     5941 acaaggcgtt tattaccgag cagtttatca actggtcgct ctctgactcg gtcacgcgcg
     6001 tggtgttgac gataccggcc cctgccgatg ccaatagcga agaagtgacg gaaatcctgc
     6061 tcaccgcagc gcgtcgctgc tcgctggtga tcgacaaccc ggcaccggaa gtcttcctgg
     6121 tggatctgca acaggggatt cagattttcg agctgcgtat ttacgccgct gagatgggtc
     6181 accgtatgcc gctacgccat gagatccacc agctgattct ggctggcttc catgcccacg
     6241 gtatcgatat gccattcccg cccttccaga tgcgtctgga aagcctcaac ggtaaacaaa
     6301 cggggagaac gctgacgtct gcgggcaaag gtcgtcaggc gggaagtttg taagtttgtg
     6361 tgccggatga accaatccgg ctcacaaaca aaactgatag cgcagaaaaa actgccacag
     6421 ccagtagccg aaaatagcca gcgcggtggc gtacataaac cagccgcgtt tttttcgcca
     6481 caaagcgaac cagagaaaag ggaaaaagag cggtaacgtc attatcacac caacatcacg
     6541 ggtatcgtct tcttcgagat acatcaacac atcgcagacg ttgttaatcc cgcgtccatc
     6601 aatcatccac tctttgtcca gcgcggaagt gatggttatc cagaggaacg ccacaataaa
     6661 ccagatgcag cataagacaa acatgcgcgc actcataagg taaccggtcg cttaatttcg
     6721 ctatagcgca tacgaaacaa atcgccgtat cccactaatt ccccgcgaaa aaccagacat
     6781 cggataagat aagcgcaata aatatccggc aggtttcccc accgtttacc atcgcgatct
     6841 ttgagggtgt taatggtgct acccaccacc atcgccactt tacgggtgtg gcaggcatcg
     6901 acaaaagaga gaatcgtctg ctctatttcc gctacatccc cggcagaaaa ttgcagcgcc
     6961 ctgatttcat cttcggttat tgccgggtcc cggctggtgt catccataac atttcctgtc
     7021 tttacgccct atcgtcaggt agacgatagg gcatcgggtt attccaccgc ccactgcgga
     7081 ttttttgcca ctctgctgca atagttctgg taaatcgcca tcgctaacag cgagaagaag
     7141 acagggccgc caatcatcca caatgtgctg tcccagtctc cggcttcaac cacaggttga
     7201 atgatggtga agacgttcgc aaatgtcacc accagtacga caaccactgt cgcaatcatt
     7261 gccgacaaat gcgttttgaa aatcacaaac ggtctgtcga gatcctgacg tgctttaaag
     7321 aacgggaaag ccagcgcgag gaacaggtaa ggaagcgtca tagacacgtt cgccatcagc
     7381 gtcagcttgt taaagaacgc cgatgcggta ccgccaccaa acgaaaccag caggatgaag
     7441 acagtaacca acccgcactg catccacatg gcgatagacg gcatccccat cgcattcagg
     7501 cgcgtcatcg gttccggcca caatgctttc ggcgtcccct ggatgatggc tttcaacggt
     7561 gaatagcaaa gcgtaaagaa cgcaccggta taggcgagga acatcgaaag tccagtaata
     7621 cgcgcaaacc atacgcccag cgacaatgac gcttcaggtg acaaatgcag tgcattaccc
     7681 agcgtcatcc caaggctctt catcagcaca taggtaatat tgccgaggtt aacggaacca
     7741 ttacttaata cctgctgcca gtttgtgctg acgccccata aaaatattgc cagcgaataa
     7801 ccgattgaaa taacaatagc ggcaaaaaca atacctttgg caaagttctt ttctggattt
     7861 tccgttttat cgaccagacc accaaccgct tcaattccgc cataggcaaa aatggcaaat
     7921 accacaaacg atagcattgc cagaccggac tgataacccg ggttcggtga tgcaaggaaa
     7981 ttaatatcct gcgcgaaatg cccaccattt aataacaaaa tagtaatgct tactaacagc
     8041 aatactaaat tcagacacat tactgcaata ccgcccaccg cagtaatgcg ggcaatttta
     8101 ttaatcccct tagaagcaac gacggtgacc agaatcatcc atgccactgc cagcagacca
     8161 accacctgcg taggctccag tccggcaata cgccagtgct gggtcatgtc gctaccatag
     8221 aggaatgttg agaacggtac ccaaactttc gcggaggtac tcaccatcca gatgatataa
     8281 gaggaaaacc acataaacgt accaatgaag gcaaaacgtg gtccgacact attattcatc
     8341 caggaataga taccgccttc ttctttgcga taagcagctc ccatttcagc catcattaag
     8401 gcgaatggaa taaagaataa caatgcagaa aatatataaa agggaatcgc actataaccc
     8461 attaagtaat aagccgatgg gctattggca aatccaaata cggaagtaaa gatcatcaat
     8521 atgagtccta tcagactcat cttttttatc gtgtgaggca tgaaaccatc cttcatttca
     8581 gcgcgtgtat aacacgtgcc cactgaactg cactgcgcaa cattgagcag gcagcgtaaa
     8641 aaccaataca gaattgatac ctggatgata tcaaatatcc agtcttaaat tgtggccttt
     8701 aacggaaaaa tgaaaacata tgctacagaa tggcgcttat cacgccattc ttcgctgtta
     8761 attcagtaat ttttcagaat tatgcccggt caacgctaaa ggcgatgact tcagccagtg
     8821 tctccgcgcc cagcgccaac atcaccagac gatcaacacc taatgccacg ccggaacagt
     8881 caggcatacc gactttcaag gcttcaatca gattctggtc aatggggtgc tgcggcaaac
     8941 cgcgcgccgc gcgcttacgg ttatcttgtt caaagcgttg ttgctgctca cgggcatccg
     9001 tcaattcatg gaaaccattc gccagctcaa tacctttata ataaacctca aagcgttcag
     9061 cgacccgatg atcttcggta ctgatttgcg ccagtgatgc ctggctggct ggaaagtggt
     9121 acacaaaggt cggtttttct ttgccaatat ttggctctac gccaaaggta aacagcaatt
     9181 gtagcagcgt gtcgcggtct tcttcggtat cagcaacatt gctcaaatcc agtttcgctg
     9241 cgacttcccg cagttgcgtt ttgtcggcag agagcgggtc aatttccaga taacgcaaga
     9301 aagcttgttg ataagaaagg ctttctgctg ccgggcagtc cagcacctgt tgtaagagat
     9361 cgtccacctc gttcatcaac cggtacatat catagtgcgg tcgataccac tccagcatag
     9421 tgaactcagg gttgtgataa cgccccatct cttcattacg gaagctgcgg cacagctgga
     9481 ataccggccc acaaccggca accagcaggc gtttcatatg gtattccggg ctggtcatta
     9541 accagagatt catcccctgc gaatgcccgg ggccaacgaa acgtgtctca aacgggacca
     9601 aatgaatatc ggttaccgtc gcctggctca tacaaggcgt ctccacctcc agcactccac
     9661 gatcggcaaa gaaacgacgg atctccgcca taatcgccgc gcgttttaat aagttaggaa
     9721 tggatgcgct cggctgccag gatgccgttt cgctcatagt taaatctcca gtttttgaca
     9781 agggcacgaa gtctactcgc aacgcgacgg cgagacaaat tttacgcagg aatcaaacag
     9841 cggtgggcag tgactaaaaa aagcacgatc tgatggttta gtaattaaat taatcatctt
     9901 cagtgataat ttagccctct tgcgcactaa aaaaatcgat ctcgtcaaat ttcagactta
     9961 tccatcagac tatactgttg tacctataaa ggagcagtgg aatagcgttc gcagaccgta
    10021 actttcaggc acttaccctg aagtacgggg ctgtgggata aaaacaatct ggaggaatgt
    10081 cgtgcaaacc tttcaagccg atcttgccat tgtaggcgcc ggtggcgcgg gattacgtgc
    10141 tgcaattgct gccgcgcagg caaatccaaa tgcaaaaatc gcactaatct caaaagtata
    10201 cccgatgcgt agccataccg ttgctgcaga agggggctcc gccgctgtcg cgcaggatca
    10261 tgacagcttc gaatatcact ttcacgatac agtagcgggt ggcgactggt tgtgtgagca
    10321 ggatgtcgtg gattatttcg tccaccactg cccaaccgaa atgacccaac tggaactgtg
    10381 ggggtgccca tggagccgtc gcccggatgg tagcgtcaac gtacgtcgct tcggcggcat
    10441 gaaaatcgag cgtacctggt tcgccgccga taagaccggc ttccatatgc tgcacacgct
    10501 gttccagacc tctctgcaat tcccgcagat ccagcgtttt gacgaacatt tcgtgctgga
    10561 tattctggtt gatgatggtc atgttcgcgg cctggtagca atgaacatga tggaaggcac
    10621 gctggtgcag atccgtgcta acgcggtcgt tatggctacc ggcggtgcgg gtcgcgttta
    10681 tcgttacaac accaacggcg gcatcgttac cggtgacggt atgggtatgg cgctaagcca
    10741 cggcgttccg ctgcgtgaca tggaattcgt tcagtatcac ccaaccggtc tgccaggttc
    10801 cggtatcctg atgaccgaag gctgccgtgg tgaaggcggt attctggtca acaaaaatgg
    10861 ctaccgttat ctgcaagatt acggcatggg cccggaaact ccgctgggcg agccgaaaaa
    10921 caaatatatg gaactgggtc cacgcgacaa agtttctcag gccttctggc acgaatggcg
    10981 taaaggcaac accatctcca cgccgcgtgg tgatgtggtt tatctcgacc tgcgtcacct
    11041 cggcgagaaa aaactgcatg aacgtctgcc gttcatctgc gaactggcga aagcgtacgt
    11101 tggcgtcgat ccggttaaag aaccgattcc ggtacgtccg accgcacact acaccatggg
    11161 cggtatcgaa accgatcaga actgtgaaac ccgcattaaa ggtctgttcg ccgtgggtga
    11221 atgttcctct gttggtctgc acggtgcaaa ccgtctgggc tccaactccc tggcggaact
    11281 ggtggtcttc ggccgtctgg ccggtgaaca agcgacagag cgtgcagcaa ctgccggtaa
    11341 tggcaacgaa gcggcaattg aagcgcaggc agctggcgtt gaacaacgtc tgaaagatct
    11401 ggttaaccag gatggcggcg aaaactgggc gaagatccgc gacgaaatgg gcctggcaat
    11461 ggaagaaggt tgcggtatct accgtacgcc ggaactgatg cagaaaacca tcgacaagct
    11521 ggcagagctg caggaacgct tcaagcgcgt gcgcatcacc gacacttcca gcgtgttcaa
    11581 caccgacctg ctctacacca ttgaactggg ccacggtctg aacgttgctg aatgtatggc
    11641 gcactccgca atggcacgta aagagtcccg cggcgcacac cagcgtctgg acgaaggttg
    11701 caccgagcgt gacgacgtca acttcctcaa acacaccctc gccttccgcg atgctgatgg
    11761 cacgactcgc ctggagtaca gcgacgtgaa gattactacg ctgccgccag ctaaacgcgt
    11821 ttacggtggc gaagcggatg cagccgataa ggcggaagca gccaataaga aggagaaggc
    11881 gaatggctga gatgaaaaac ctgaaaattg aggtggtgcg ctataacccg gaagtcgata
    11941 ccgcaccgca tagcgcattc tatgaagtgc cttatgacgc aactacctca ttactggatg
    12001 cgctgggcta catcaaagac aacctggcac cggacctgag ctaccgctgg tcctgccgta
    12061 tggcgatttg tggttcctgc ggcatgatgg ttaacaacgt gccaaaactg gcatgtaaaa
    12121 ccttcctgcg tgattacacc gacggtatga aggttgaagc gttagctaac ttcccgattg
    12181 aacgcgatct ggtggtcgat atgacccact tcatcgaaag tctggaagcg atcaaaccgt
    12241 acatcatcgg caactcccgc accgcggatc agggtactaa catccagacc ccggcgcaga
    12301 tggcgaagta tcaccagttc tccggttgca tcaactgtgg tctgtgctac gccgcgtgcc
    12361 cgcagtttgg cctgaaccca gagttcatcg gtccggctgc cattacgctg gcgcatcgtt
    12421 ataacgaaga tagccgcgac cacggtaaga aggagcgtat ggcgcagttg aacagccaga
    12481 acggcgtatg gagctgtact ttcgtgggct attgctccga agtctgcccg aaacacgtcg
    12541 atccggctgc ggccattcag cagggcaaag tagaaagttc gaaagacttt cttatcgcga
    12601 ccctgaaacc acgctaagga gtgcaacatg acgactaaac gtaaaccgta tgtacggcca
    12661 atgacgtcca cctggtggaa aaaattgccg ttttatcgct tttacatgct gcgcgaaggc
    12721 acggcggttc cggctgtgtg gttcagcatt gaactgattt tcgggctgtt tgccctgaaa
    12781 aatggcccgg aagcctgggc gggattcgtc gactttttac aaaacccggt tattgtgatc
    12841 attaacctga tcactctggc ggcagctctg ctgcacacca aaacctggtt tgaactggca
    12901 ccgaaagcgg ccaatatcat tgtaaaagac gaaaaaatgg gtccagagcc aattatcaaa
    12961 agtctctggg cggtaactgt ggttgccacc atcgtaatcc tgtttgttgc cctgtactgg
    13021 taaggagcct gagatgatca atccaaatcc aaagcgttct gacgaaccgg tattctgggg
    13081 cctcttcggg gccggtggta tgtggagcgc aattattgcg ccggtgatga tcctgctggt
    13141 gggtattctg ctgccgctgg gactgtttcc gggtgatgca ctgagctacg agcgcgttct
    13201 ggcgttcgcg cagagcttca ttggtcgcgt attcctgttc ctgatgatcg ttctgccgct
    13261 gtggtgtggt ttacaccgta tgcaccacgc gatgcacgat ctgaaaatcc acgtacctgc
    13321 gggcaaatgg gttttctacg gtctggctgc tatcctgaca gttgtcacgc tgattggtgt
    13381 cgttacaatc taacgcatcg ccaatgtaaa tccggcccgc ctatggcggg ccgttttgta
    13441 tggaaaccag accctatgtt caaaacgacg ctctgcgcct tattaattac cgcctcttgc
    13501 ttcacatttg ctgcccccca aaaaatcaac gatattgtgc atcgcacaat taccccgctt
    13561 atagagcaac aaaagatccc cggtatggcg gtggcggtaa tttatcaggg taaaccttat
    13621 tactttacct ggggctatgc ggacatcgcc aaaaagcagc ccgtcacaca gcaaacgttg
    13681 tttgagttag gttcggtcag caaaacattt acgggcgtgc ttggtggcga cgctattgct
    13741 cgaggggaaa tcaagttaag cgatcccgca acaaaatact ggcctgaact taccgctaaa
    13801 cagtggaacg ggatcacact attacatctc gcaacctaca ccgctggcgg cctgccattg
    13861 caggtgccgg atgaggtgaa atcctcaagc gacttgctgc gcttctatca aaactggcag
    13921 cctgcatggg ctccaggaac acaacgtctg tatgccaact ccagtatcgg tttgttcggc
    13981 gcactggctg tgaagccgtc tggtttgagt tttgagcagg cgatgcaaac acgtgtcttc
    14041 cagccactca aactcaacca tacgtggatt aatgtaccgc ccgcagaaga aaagaattac
    14101 gcctggggat atcgcgaagg caaggcagtt catgtttcgc caggggcgtt agatgctgaa
    14161 acttatggtg tgaagtcgac cattgaagat atggcctgct ggatacgaag caatatgaat
    14221 ccccgtgata tcaacgacaa aacacttcag caagggatac aactggcaca atctcgctac
    14281 tggcaaaccg gcgatatgta tcagggcctg ggctgggaaa tgctggactg gccggtaaat
    14341 cctgacagca tcattaacgg cagtggcaat aaaattgcac tggcagcacg ccccgtaaaa
    14401 gcgattacgc ccccaactcc tgcagtacgc gcatcatggg tacataaaac aggggcaacc
    14461 ggcggatttg gtagctatgt cgcgtttatt ccagaaaaag agctgggtat agtgatgctg
    14521 gctaacaaaa actatcccaa tccagcgaga gtcaccgccg cctggcagat tctcaacgct
    14581 ctacagtaaa atttcatcgg gtccgaattt tcggaccttt tctgcgcttt tccttgctgt
    14641 catctacact tagaaaaaaa ccagtaagga aacatttatg cgcctgctcc ctctcgttgc
    14701 cgcagcgaca gctgcatttc tggtcgttgc ctgcagttct cctacgccgc cgcgtggcgt
    14761 gacagtagtg aataatttcg acgctaaacg ctatctcggt acctggtatg agattgcccg
    14821 ttttgatcac cgttttgaac gtggactgga aaaagtcacc gcaacataca gtctgcgtga
    14881 tgacggcggc ctgaatgtca tcaataaagg ctataaccct gacagagaaa tgtggcagca
    14941 gagtgaaggg aaagcgtact ttaccggcgc accaacacgc gctgcgctga aagtgtcatt
    15001 ctttggtcct ttctatggcg gttataacgt cattgcactt gatcgggaat accgccatgc
    15061 gctggtttgc ggcccggacc gcgactacct gtggatactc tcccgcacgc caaccatttc
    15121 tgacgaagtg aaacaggaga tgctggcagt cgcgacccgg gaagggtttg atgtcagtaa
    15181 gtttatttgg gtacagcagc ccggtagtta gtgagtgctg agtttcagac caataatccc
    15241 caatacgatt aacgccagac tcgccaggcg catcgggtta gcggactcac cgagcagtac
    15301 aatgccggtg atagccgcgc cgactgcgcc aatacccgtc cacacggcat aagccgtccc
    15361 tactggtaac gatttcatcg cccaggcaag tagcgccata ctgacaatca tcgccgtcac
    15421 ggtaataaca ctcggcgtca aacgactaaa gccgtgggta tatttcaggc caacggccca
    15481 taccacttcc agcagaccag caataactaa gataatccag gacatatcag gctccggaga
    15541 attggggccg tccccggtga aaagacgcgt ttgcaggtcg tcctgcaaag ctgatggttg
    15601 aacggatatt ttgccaggtg gcaggggaat ttcaactatt accttagtga ctatcgcaga
    15661 aaaaacaaac ggcacgacac agctgtcgta ccgttattgc ttattgctat tgctgcgctt
    15721 tcgttgctgc gccagaaatc gcgttaccgc catcagaaat gtcttcacca acgccacgcg
    15781 tggtgttgca ggcagttaat actgttgaaa gcaccagaac agaaaagatc gctgcaattg
    15841 tcttcttcac cataacgtct tccttttagg taatgttatt tatgtttgcc tatagcaaat
    15901 taataataga caacatcgcc aaaaatgacg gaaatctgat gatttttcgg aagagaagaa
    15961 aaattagctg gcagcgcgag agatggagtt gccgagatgt ttgatgtctt cgccgaaacc
    16021 gcgagcggtg ttacagcccg tgagcagcgt gctggcaagc aaaacaagaa cgataaggcg
    16081 tttcatcatc tctgacctga ataagtgatg gtgcagcctg caggccgcac cacaaccgca
    16141 ttacttcacg cgagagacgt attcaccaga gcgggtatcc actttgatga cttcgccgat
    16201 ttgtacgaac agcggaacct taaccacagc gccagtagac agggtagccg gtttgccgcc
    16261 agtacctgcg gtatcacctt tcaggcccgg atcggtatca acgatttcca gttcaacgaa
    16321 gttcggcgga gtaacggaga tcggctgacc attccacaga gttacgatac actctgcctg
    16381 atccagcagc catttagcgt tatcgccaat tgcttttgca tcagcagaca gctgctcgaa
    16441 agtttcgttg ttcatgaagt gccagaactc accgtcgttg tacaggtaag tcaggttcat
    16501 atcgacaaca tcagcgcctt cagcggaatc agtagatttg aaggtttttt ctacgcgagt
    16561 accggtcagc agacgacgca gtttaacgcg agcaaatgcc tggcctttac ccggttttac
    16621 gaattcactc gcttcaaccg cgtaaggttc gccgtctaac atgattttaa gaccagcacg
    16681 aaaatcgttg ctatagtacg ttgccataag gccctctgaa atttgttaat tggtagctaa
    16741 gccacaaaat ggcgcatatt gtaaccctaa ataccccatc cagagaagat tggttaacgc
    16801 aacttgccga tgttgtgacc gatcctgatg aacttctgcg tcttttgaat atagacgcgg
    16861 acgaaaaact gttggccgga cgcagcgcca aaaagctgtt tgccctgcgt gtgccccgct
    16921 catttatcga tcgcatggag aaaggcaatc cggacgatcc tcttttgcgt caggtactta
    16981 cctcgcaaga tgagtttgtc gtcgcgtccg gattctccac cgacccactg gaagaacagc
    17041 acagcgtagt gcctggtttg ttgcataaat accacaaccg ggcgcttttg ctggtcaaag
    17101 gcggctgcgc ggtaaattgc cgctattgct tccgtcgcca cttcccctat gccgaaaatc
    17161 agggcaacaa gcgtaactgg cagactgcac ttgagtatgt tgctgcgcat ccggaactgg
    17221 acgagatgat tttctctggc ggcgatccgc tgatggcgaa agatcacgag ctggactggt
    17281 tgctcacaca actggaagcc atcccgcata tcaaacgtct gcggattcac agccgtctgc
    17341 cgattgtgat cccggcacgc atcacagatg cgctggttga acgcttttca cattctacgc
    17401 tgcaaatctt gctggtgaat cacatcaacc atgccaatga gatagatgaa acattccgtc
    17461 aggcgatggc taagttgcgc cgggtaggcg ttactttgct gaaccagagc gttctgttac
    17521 gtgatgtgaa cgataacgca caaacgctgg caaacctgag taatgcgttg ttcgatgccg
    17581 gcgtgatgcc gtattatctg catgtgctcg ataaagtaca gggcgcggcg cattttatgg
    17641 tgagtgatga cgaagcacgg cagattatgc gtgagttgct gacattggtg tcgggatatc
    17701 tggtgccgaa actggcgcga gaaattggcg gcgaacccag caaaacgccg ctggatctcc
    17761 agctacgcca gcagtaactg attaaacagg gaagttaaac attaaaattg cctgatgcgc
    17821 tacgcttatc aggcctacat cgtgcattga aattgaatgg atttgcaatg aactgtaggc
    17881 cggataaggc gtttacgccg catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc
    17941 attaaactga gaaatttaat ctttgcacgt tgcataacct ttaattattc atatgcattg
    18001 ctgttgttac tttttatata gctaacacca ataattaacc
//
LOCUS       DAPHBJ010000491        11027 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.guided.145, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000491 DAPHBJ010000000
VERSION     DAPHBJ010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.guided.145"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(36..1748)
                     /locus_tag="RHZ76_004732"
                     /pseudo
     CDS             complement(36..1748)
                     /locus_tag="RHZ76_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            1720..2505
                     /gene="lafU"
                     /locus_tag="RHZ76_004733"
     CDS             1720..2505
                     /gene="lafU"
                     /locus_tag="RHZ76_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HDV2815205.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRAFSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            2576..3631
                     /gene="dinB"
                     /locus_tag="RHZ76_004734"
     CDS             2576..3631
                     /gene="dinB"
                     /locus_tag="RHZ76_004734"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDV2815206.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            3683..3976
                     /gene="yafN"
                     /locus_tag="RHZ76_004735"
     CDS             3683..3976
                     /gene="yafN"
                     /locus_tag="RHZ76_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HDV2815207.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEKYLNDMTDDDFNDFKE"
     gene            3979..4377
                     /gene="yafO"
                     /locus_tag="RHZ76_004736"
     CDS             3979..4377
                     /gene="yafO"
                     /locus_tag="RHZ76_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HDV2815208.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKQARDNNQMHKIGKMAEAFRMRF"
     gene            4387..4839
                     /locus_tag="RHZ76_004737"
     CDS             4387..4839
                     /locus_tag="RHZ76_004737"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV2815209.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMISSQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITCVERYIDMLFVDPEYTRRGVACALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQY"
     gene            5085..5288
                     /locus_tag="RHZ76_004738"
                     /pseudo
     CDS             5085..5288
                     /locus_tag="RHZ76_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="mutations in this gene result in a compromised
                     ability for drug-inducible mexXY expression; expression is
                     inducible by the same ribosome-targeting agents that
                     induce mexXY; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            5287..5808
                     /locus_tag="RHZ76_004739"
                     /pseudo
     CDS             5287..5808
                     /locus_tag="RHZ76_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5865..7322)
                     /gene="pepD"
                     /locus_tag="RHZ76_004740"
     CDS             complement(5865..7322)
                     /gene="pepD"
                     /locus_tag="RHZ76_004740"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDV2815210.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQDILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIKSVGHYWTLLTELLKEIPAK"
     gene            7583..8041
                     /gene="gpt"
                     /locus_tag="RHZ76_004741"
     CDS             7583..8041
                     /gene="gpt"
                     /locus_tag="RHZ76_004741"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDV2815211.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            8133..9377
                     /gene="frsA"
                     /locus_tag="RHZ76_004742"
     CDS             8133..9377
                     /gene="frsA"
                     /locus_tag="RHZ76_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDV2815212.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            9435..9836
                     /gene="crl"
                     /locus_tag="RHZ76_004743"
     CDS             9435..9836
                     /gene="crl"
                     /locus_tag="RHZ76_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDV2815213.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(9875..10930)
                     /gene="phoE"
                     /locus_tag="RHZ76_004744"
     CDS             complement(9875..10930)
                     /gene="phoE"
                     /locus_tag="RHZ76_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HDV2815214.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
BASE COUNT         2689 a         2778 c         2828 g         2732 t
ORIGIN      
        1 gatgcctgat gcgacgctga cgcgtcttat catgcctaca tcaacgcccc cataatcttc
       61 aactccagct catccggcac ttcgttatac gacagcacat gcagccccgg cgcaaacaac
      121 cttgcataac gcgccagcaa agggcgcagc tgcggtggca ccagcagcac cgggtctttc
      181 cccgccgctt tcatctgctc tttcacctgt ggcatcgtgc tctggaactg gttgagcata
      241 ttcgggtcca ctggcacgct gtcaagcatc actttcccgc cctgttgcgc ctgattcacc
      301 acattggtca gcagattttc cagttcatta ttcagcgtat acaccgtcag ctcctgcttg
      361 cgaacgaacg gatgggtaat gctgcgccgc agcgccaggc gcacatcagc tgccagcaga
      421 atatgatctt tggtcaccgc gctactggcg accagcacgg tggcgatggt gacgatatcg
      481 cgcagggaaa cgccttcggt cagcagcgcc cggtacactt tcagcaactg gctgtaattg
      541 agcgccgcgc ttaaatcttc cgccaggcgc ggtgccatcg acgccaaacg gttatgcaac
      601 tgcgtaatat catcatagtt aaacaaatca ggaatatagc tgcgcacaat cttgttcaca
      661 tgggtagcaa tcacgctggc gctgtcgatc acctgatacc ccatattcag cgccttcgcc
      721 ttctgcgcag gctggatcca ggtgaccggc atcccatacg ccgggtcgtt ccccagcacg
      781 ccgtcaatct cgccgtaggt ttcgctggag ggcagggcca tcagtttatc cgccggaata
      841 tccgcttcat cagccttaat gccgttgatg aaaatagcgt actggctggg cttaaggcgg
      901 aagttttccc gaatgcggat ctccggcagc agcacgccgt tgccgtcaga aatcacctgt
      961 cgcacgccgc gaatacgctg ggtgagcggg ttgccctggg ctttgtccac cagcgccacc
     1021 agcttgtagc cgaggcttaa gctgatgggc tcgatcagcg gaatggtttc ccaactgacc
     1081 tgttgctcgc tggtttcggt gatggtgcgg gtcagcgttt cgagactttt ctcttccgct
     1141 tccgccgcct gcgggcgttt gctcatccgc cagccggtaa agccaagcag ggcgctgaac
     1201 agcaaaaacg gcagatgcgg cattcccggc accatcgcca gcacaaacat aatcccggta
     1261 gcggtgtaga gcaccgacgg gcttgccagc agctggtggc gcacgtcatg ggcgatatcg
     1321 ccgttgtcgc tgacgcgggt gacgataatc gccgccgccg tggagagcag cagggaaggg
     1381 atctgcgcca ccaggccgtc gccgatggtc atcagcacat actgctggaa ggcagcatcg
     1441 gcgctcaggt tgtatttgaa gatcccgata cagacgccgc cgatcaggtt gatcgccaga
     1501 atcatcatcc cggcgatggc gtccccgcgc acaaacttcg atgccccgtc catcgcgccg
     1561 tagaaatcgg cctcgctggc aacatcttta cgccgggttt gcgcctgcgc ctggttgatc
     1621 aatccggcgt taagatcggc gtcaatcgcc atctgtttgc cgggcatcgc gtctaaggta
     1681 aagcgggcag aaacctcgga aatacgctcg gcccctttgg tgacgacaat aaagttgatt
     1741 gtcaacagcg tcagtaaatc cgagcgtgaa agcattatcg ccgcgctgca cgggcagtcg
     1801 atttttaacg gtggtgggct gtcgccgtta aataaaatca gcccgtctca tccgccaaaa
     1861 cctgccaccg tggcggtgcc ggaagagacg gagaaaaaag cgcgcgacgt caacgagaaa
     1921 acggcgctgc tgaagaagaa atccgccacc gagcttggcg agctggcaac cagtatcaac
     1981 accattgccc gtgatgcgca tatggaagcg aatctggaga tggagattgt tccccaggga
     2041 ttgcgcgtgc tgattaaaga tgaccagaac cgcaatatgt ttgaacgcgg cagcgcgaag
     2101 attatgccgt tctttaaaac gctgctggtg gagctcgcgc cagtgttcga ctcgctcgat
     2161 aataaaatta ttattaccgg gcataccgat gcgatggcct acaaaaacaa tatctacaac
     2221 aactggaacc tttcgggtga ccgcgcgttt tcggctcgtc gggtgctgga agaggccgga
     2281 atgccggaag ataaagtgat gcaggtaagc gcaatggcgg accagatgct gctggacgcg
     2341 aagaatccgc aaagtgcggg taatcggcgc atcgagatta tggtgctgac caaaagtgcg
     2401 tccgatacgc tgtatcaata ctttggtcag catggggata aagtggtgca gccgctggtg
     2461 caaaagctgg ataagcagca ggtgctttcg cagcgaacgc gctaaatgct gaatctttac
     2521 gcatttctca aaccctgaaa tcactgtata ctttaccagt gttgagaggt gagtaatgcg
     2581 taaaatcatt catgtggata tggactgctt tttcgccgca gtggagatgc gcgacaatcc
     2641 cgccctgcgc gatatcccta ttgctattgg cggcagccgc gaacgtcggg gggtgatcag
     2701 caccgccaat tatcccgcgc gtaaatttgg cgtacgtagc gctatgccga cagggatggc
     2761 gctcaaatta tgcccgcatc tcaccttgct tccggggcgc tttgacgcct acaaagaagc
     2821 ctcaaatcat atccgcgaaa tcttctcgcg ctacacctcg cgtattgaac cgttgtcact
     2881 ggatgaggct tatctcgacg tcaccgatag cgtccattgc cacggttctg cgaccctcat
     2941 cgcccaggaa atccgccaga cgattttcaa cgagctgcaa ctgacggcgt ctgcgggcgt
     3001 ggcacccgta aagtttctcg ccaaaatcgc ctccgacatg aataaaccca acggccagtt
     3061 tgtgattacg ccggcagaag ttccggcatt tttacaaacc ttaccactgg caaaaatccc
     3121 cggcgtcggc aaagtctcgg cggcaaaact ggaagcgatg gggctacgaa cctgcggtga
     3181 tgtacaaaag tgtgatctgg tgatgctgct taaacgcttt ggcaaatttg gccgcatttt
     3241 gtgggagcgt agtcagggga ttgacgagcg cgacgttaac agcgaacggt tgcgaaaatc
     3301 cgtcggcgtg gaacgcacga tggcggaaga tatccaccac tggtctgaat gtgaagcgat
     3361 tatcgagcgg ctgtatccgg aacttgaacg ccgtctggca aaggtaaaac ctgatttact
     3421 gattgcccgc cagggggtga aattaaagtt cgacgatttt cagcaaacca ctcaggagca
     3481 cgtctggccg cggctgaata aagctgactt aatcgccacc gcgcgtaaaa cctgggatga
     3541 acgccgcggc gggcgcggtg tgcgactggt ggggctgcat gtgacgttgc ttgatccgca
     3601 aatggaaaga caactggtgc tgggattatg atgtatacta ttatgtctat tctggtgtgc
     3661 attattatga ggggatcact gcatgcatcg aattctcgct gaaaaatcgg tcaatattac
     3721 tgagttacgt aaaaacccag ctaaatactt tattgatcaa ccggttgcgg ttctttctaa
     3781 taatcgcccc gcaggatatc tcttaagtgc cagcgcattc gaagcgttaa tggacatgct
     3841 tgctgaacaa gaggagaaaa agcccataaa ggcgcgcttc cgtccaagtg ctgcaagatt
     3901 agaggaaatt acacgccgag ctgaaaaata tcttaatgat atgacggatg atgatttcaa
     3961 tgactttaag gaataaggat gcgggtattc aaaacaaaac ttattcgcct gcaacttaca
     4021 gcagaggaac ttgatgcgtt aacggcggat tttatttcct ataagcgtga cggtgttttg
     4081 ccagatatat ttggtcgcga tgcactctac gacgactcgt ttacctggcc attaatcaaa
     4141 tttgagcgag ttgctcatat tcatctggca aatgtgaata atccatttcc gccacagttg
     4201 cgccaattca gcagaacgaa tgacgaagcg catttggtat attgtcaggg tgcgtttgat
     4261 gagcaagcat ggttgctcat tgccattctg aaacctgaac ctcataaaca ggctcgagat
     4321 aacaaccaaa tgcataaaat tgggaaaatg gcagaagcgt ttcgcatgcg tttttgaatt
     4381 tatattatga ataacataca aataagagac tatcagcctg gcgattttca gcaactatgc
     4441 gctattttca ttagagcggt tacgatgatc tccagtcagc attattcacc acaacaaatt
     4501 gccgcctggg cgcagattga cgaatctcgc tggaaggaga aactcgcgaa atcacaagtg
     4561 cgggttgccg tcattaatgc acaaccggtt ggttttatta cctgcgttga acgttatatc
     4621 gatatgttat ttgttgaccc tgaatacacc cgccgcgggg ttgcctgcgc tttgttaaaa
     4681 cctttgatta agtctgaatc cgaacttacg gtggacgcca gcataaccgc aaaacccttt
     4741 tttgaacgtt atggttttca gacagttaag cagcagcgcg ttgaatgccg gggaacgtgg
     4801 tttactaatt tttatatgcg atataaaccg caatattaaa tccagcttgc aatgaaaata
     4861 acgcccgcct ggtatgtgcg cgttttctta ttccacaaac tgcaaggagg taaatcatga
     4921 caaatccttt atcaatgact ctttgcagac ctttccagga ttaattcttt ttttcttgcc
     4981 ctggattcgt ctgccatttc ctgattttta tatttatata ctctaaataa ttcgagtcgc
     5041 agtacatgca acttgaagta tgacgagtat aaatggaatg gtacatgggc aaatatattc
     5101 gtcccttatc cgatgcggta tttaccatcg catctgatga cctgtggatc gagagtttag
     5161 cgatccaaca attacacacc acggcaaatt tacccaacat gcagcgcgta gtggggatgc
     5221 cagatttaca ccccggacgc ggctacccga ttggcgcagc gttcttctct gttggtcgtt
     5281 tttacccgac aagacgtcgc tgtaacggtg ctggaaacag aaacgggccg ctactctgac
     5341 acgctgcgtt cggcgctggt ttctctggat ggcgataacg catgggcatt aagcgaaagc
     5401 tggtgtggca ctattcagtg gatttgtccg agtccgtatc ggcctcatca tgggcgcaaa
     5461 aactggtttc tgggcattgg gcgttttacc gctgatgaga aggaacaatc ggatgcaatc
     5521 cgttatgaga cgctgcgttc gtcggggccg ggcggtcaac atgtcaataa aaccgactcg
     5581 gcggtacgcg ccacgcatct ggcatccggt attagcgtga aggttcagtc agagcgcagc
     5641 cagcatgcta acaaacggct ggcgcgattg ctgattgcct ggaagctgga gcagcagcaa
     5701 caggaaaata gcgcggcgct gaaatcgcag cggcgaatgt tccatcacca gattgaacgt
     5761 ggcaacccgc gacggacatt tacagggatg gcttttatcg aaggataatg aaggatgaaa
     5821 ctgccggaag gcgattaaac gccatccggc agcaaatcaa ataattactt cgccggaatt
     5881 tctttcagca gttcagtcag cagtgtccag taatgaccta cgcttttgat gtgaacttgc
     5941 tcatccggag agtgtggacc ggtgatagtt ggcccgatag aaaccatgtc catttccgga
     6001 tacggttttt taaacagacc acattccagg cccgcgtgga taatctggat gttcggcgtc
     6061 ttgttgaaca ggcgctgata ggtttcacgt accagatgca tcaccggaga attagcgtcc
     6121 ggctgccagc caggatatgc gcctttcgct tcggttttcg cgccagccag tttacccagc
     6181 gaatccagca tgctcaccac gtagtcttta ccgctgtcga tcagtgaacg gatcaggcag
     6241 tgaatttcta cgttattgtc agtcatggtc accacaccga cgttcaggga ggtttcgacc
     6301 acaccttttg ccacgtcgga attgcggatc acaccgttcg gggtggcgtt cagcagacga
     6361 ataaaggtat cgcgagattt cgcaatcaga gcagctttat cgttcgctac agagtccagc
     6421 aacaaggcca gattcttctc tttctctgcc agctcgtttt tcaggatgtc ctgataggta
     6481 ttcaccagag atttcagggc gtcgacttta tcagctgcga cagcaatggt cgcaaaggct
     6541 tcacgcggga tggcgttacg cagtgtgcca ccgttgaaat cgataaggcg caggtccagc
     6601 tcttccgcat gacccgccag gaagcgcacc agcagtttgt tggcattacc caggccaacg
     6661 tggatttcac cgccggagtg accgcctttt agacctttta aggttaactt gaaggtttca
     6721 aagccagctg gaaccgcttc acgatccaaa tgcaggttgg aggtgaagtc gatacccccc
     6781 gcgcaaccca tgtagatttc accttcttct tcggagtcgg tgttaatcag aatatcagcc
     6841 tgcaaccagt tgctctgtaa gccgaacgca ccgtccatac cggcttcttc ggtcatggtc
     6901 agcagcactt ccagcgggcc gtgaaccacg ttttcgtcag ccagaaccgc cagcgcagac
     6961 gccataccaa tgccgttatc tgcacccagc gtggtgccgc gcgctttaac ccattcgcca
     7021 tcaatataag gctggatagg atctttcgtg aagtcatgca cggtgtcgtt atttttctgc
     7081 ggcaccatat cgaggtgggc ctgcaagacg accggtttac gattttccat acctgcggtg
     7141 gcaggtttac gaatcaggat attacctacc tgatcgcgtt cgacatggaa acctttctct
     7201 tttgcccaac caacaatgta ttcagcgagt tgctcttcat gataggacgg gtgaggaata
     7261 gaacagattt tggcaaaaat atcccacagc ggctgtggag ataattgaga cagttcagac
     7321 acgttaagtc tccttgtcga tcacccgcaa aacagtattg caggtcacag ggttagcaga
     7381 aaatgttgtc aacacaagac aggcttgcga gatatgtttg agaataccac tttatcccgc
     7441 gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg tttttagtgc
     7501 gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt aagccgtaga
     7561 taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg acatgttgca
     7621 gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga aaggcattat
     7681 tgccgtaagc cgtggcggtc tggtaccggg tgcgttactg gcgcgtgaac tgggtattcg
     7741 tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt
     7801 gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac
     7861 cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt
     7921 cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac
     7981 ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta
     8041 atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat
     8101 agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc
     8161 tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg
     8221 gcgcacaacc gcctgtgcag tcggctcttg atggtaaaac catccctcac tggtatcgca
     8281 tgattaaccg tctgatgtgg atctggcgcg gtattgaccc acgtgaaatc ctcgacgtcc
     8341 aggcacgtat tgtgatgagc gatgctgaac gtaccgacga tgatttatac gatactgtga
     8401 ttggctaccg tggtggcaac tggatttatg agtgggccac ccaggcgatg gtgtggcaac
     8461 aaaaagcctg tgcggaagaa gatccgcaac tcagtggtcg tcactggctg catgcggcta
     8521 cgttgtataa cattgctgcc tatcctcatc tgaaaggaga tgacctggcc gagcaagcgc
     8581 aggctttgtc aaaccgcgcc tatgaagagg ccgctcagcg tctgccgggc acgatgcggc
     8641 agatggagtt taccgtaccc ggcggtgcgc ccatcaccgg ctttttgcat atgccgaaag
     8701 gcgatggtcc gttcccgaca gtattaatgt gtggtggtct ggatgcgatg cagacggact
     8761 attacagcct gtacgaacgt tattttgcgc cgcgcggcat tgcgatgctg actattgata
     8821 tgccgtcggt gggcttttct tcaaaatgga agctgaccca ggactccagc ctgttgcatc
     8881 agcacgtctt aaaggcgctg cctaacgtac cgtgggtgga tcacactcgc gtcgcggcct
     8941 ttggtttccg tttcggcgct aacgttgccg tgcgtctggc ataccttgaa tcgccgcgtc
     9001 tgaaagcggt tgcctgtctt ggtccggtag ttcataccct gttgagtgat tttaagtgcc
     9061 agcaacaggt gccggaaatg tatcttgacg ttctggcgag tcgtttgggg atgcatgatg
     9121 cttccgacga agcgttgcgc gtggagctga atcgctattc attaaaagtg caaggattgc
     9181 tgggacgtcg ctgcccaacg ccaatgttat cgggctactg gaagaacgat ccgttcagcc
     9241 cggaagagga ctcacgctta atcacctcat catctgctga cggtaaatta ttagagatcc
     9301 catttaaccc ggtgtatcgg aattttgaca aaggtcttca ggaaatcaca gactggatcg
     9361 aaaaacgttt gtgttaaaaa tttgctaaaa tttgccaatt tggtaaaaca gttgcatcac
     9421 aacaggagat tgcaatgacg ttaccgagtg gacacccgaa gagcagattg atcaaaaaat
     9481 ttaccgcact aggcccgtat attcgtgaag gtaagtgcga agataatcga ttctttttcg
     9541 attgtctggc tgtatgcgtc aacgtgaaac cggcaccgga agtgcgtgag ttctggggct
     9601 ggtggatgga gcttgaagcg caggaatccc gttttaccta cagttaccag tttggtctgt
     9661 tcgataaagc aggcgactgg aagagtgttc cggtaaaaga cactgaagtg gttgaacgac
     9721 tggagcacac cctacgtgag tttcacgaga agctgcgtga actgctgacg acgctgaatc
     9781 tgaagctgga accggcggat gattttcgtg atgaaccggt gaagttaacg gcgtgagtga
     9841 aatgtgccgg atgtatcaca tccggcaata tccattaaaa ctgataggtc atgccaaccg
     9901 cgacaatatc atcattatta atattcaatt tgttatcgct atccagttgg ttgattttat
     9961 aatcaacaaa ggctgacata tttttgttga aataatacgt agcaccgacg tcgatataat
    10021 tgaccagatc ttcatcaccg ataccttcaa tatctttccc tttcgataag acataaccca
    10081 gcgatggacg cagaccaaag tcaaactggt attgagcgac cgcttcaaag ttctgtgtct
    10141 tattggcaaa gccgccagtt attggcgtca ttttgcgtgt ttcagaatag aaagttgcca
    10201 gataaatatt attggcatcg tatttcagac ctgttgccca tgcttctgca cgcttgcctg
    10261 tgccacggct ttgcaggttc tgctcgttgg tgcgatctga gttggtatag gccccactaa
    10321 tggcgaaatc gctgccgcca aagtcatatg tcaatgacgt gccgaagcca tcgccgtttt
    10381 gctttttaac gtcgcggttt tcgtttttcc cttgatattg cagggttaag ttcaggccat
    10441 cgataacgcc gaagaagtcg gtgttccgat acgtcgccag accgctggcg cgtttggtca
    10501 taaagttgtc ggtctgcgcc gaggaatcgc caccaaattc cgggaacata tcggtccagg
    10561 cttccacgtc atacaacgcg cccaggttac gaccataatc gaaagaaccc aaatctttat
    10621 atttcaaccc ggcaaaagcg agacgcgttt tttgctgtgc agtatcactc tctgctttat
    10681 taccggcaaa ctctgcttcc caacgaccat aaccagtcag ttgatcgtta atttgtgttt
    10741 cgcctttaaa accaaaacgg atataactct ggtcgccatc tttactggcg ttatcactca
    10801 tataatgcat ggctttaact ttgccataga catccagttt attaccgtct ttattatata
    10861 tttctgcagc ctgtacagat gcagatgcca caatgcccat caccactaat gccagagtgc
    10921 tctttttcat tttcattcct gattttaatt aacgcgcgaa tattcagcgg gagagtcccg
    10981 ttgaaaacag gaaagttttt aacctgagat tgttaaagat atattac
//
LOCUS       DAPHBJ010000492         3610 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10729
            SAMN37211584-rid19351653.guided.280, whole genome shotgun sequence.
ACCESSION   DAPHBJ010000492 DAPHBJ010000000
VERSION     DAPHBJ010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211584
            Sequence Read Archive: SRR25824741
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875465.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 07:15:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,666
            Genes (coding)                    :: 4,509
            CDSs (with protein)               :: 4,509
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 43 of 157
            Pseudo Genes (multiple problems)  :: 14 of 157
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211584-rid19351653.guided.280"
                     /isolate="ARS-CC10729"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            85..990
                     /locus_tag="RHZ76_004745"
     CDS             85..990
                     /locus_tag="RHZ76_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HDV2815215.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            987..2171
                     /locus_tag="RHZ76_004746"
                     /pseudo
     CDS             987..2171
                     /locus_tag="RHZ76_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            2062..2514
                     /locus_tag="RHZ76_004747"
     CDS             2062..2514
                     /locus_tag="RHZ76_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013023839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HDV2815216.1"
                     /translation="MRPARLSTLLVRSLASLYRPCLRGSWLYSRSVFNLLPGTYVRPH
                     RHPHTFELLLPLRGRFVVLNFDDRGTVTHRAILGETCTVLEMAAGTWHAVLSLDTGGI
                     IFEVKHGGYQPVAADDYAHWAPAEGEPGTTELMAWYAQAQVGDSAFAV"
     gene            complement(2561..3436)
                     /locus_tag="RHZ76_004748"
     CDS             complement(2561..3436)
                     /locus_tag="RHZ76_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-1"
                     /protein_id="HDV2815217.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQS
                     GGRLGVALINTADNSQILYRADERFAMCSTSKVMAVAAVLKKSESEPNLLNQRVEIKK
                     SDLVNYNPIAEKHVDGTMSLAELSAAALQYSDNVAMNKLISHVGGPASVTAFARQLGD
                     ETFRLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNT
                     TGAASIQAGLPASWVVGDKTGSGDYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESR
                     RDVLASAAKIVTNGL"
BASE COUNT          632 a         1093 c         1065 g          820 t
ORIGIN      
        1 gacaaaaggc tgcctcatcg ctaactttgc aacagtgccg ttacatttag gtggctaaac
       61 ccgtcaagcc ctcaggagtg aatcatgacc gtagtcacga ccgccgatac ctcccaactg
      121 tacgcacttg cagcccgaca tgggctcaag ctccatggcc cgctgactgt caatgagctt
      181 gggctcgact ataggatcgt gatcgccacc gtcgacgatg gacgtcggtg ggtgctgcgc
      241 atcccgcgcc gagccgaggt aagcgcgaag gtcgaaccag aggcgcgggt gctggcaatg
      301 ctcaagaatc gcctgccgtt cgcggtgccg gactggcgcg tggccaacgc cgagctcgtt
      361 gcctatccca tgctcgaaga ctcgactgcg atggtcatcc agcctggttc gtccacgccc
      421 gactgggtcg tgccgcagga ctcggaggtc ttcgcggaga gcttcgcgac cgcgctcgcc
      481 gccctgcatg ccgtccccat ttccgccgcc gtggatgcgg ggatgctcat ccgtacaccg
      541 acgcaggccc gtcagaaggt ggccgacgac gttgaccgcg tccgacgcga gttcgtggtg
      601 aacgacaagc gcctccaccg gtggcagcgc tggctcgacg acgattcgtc gtggccagat
      661 ttctccgtgg tggtgcatgg cgatctctac gtgggccatg tgctcatcga caacacggag
      721 cgcgtcagcg ggatgatcga ctggagcgag gcccgcgttg atgaccctgc catcgacatg
      781 gccgcgcacc ttatggtctt tggtgaagag gggctcgcga agctcctcct cacgtatgaa
      841 gcggccggtg gccgggtgtg gccgcggctc gcccaccaca tcgcggagcg ccttgcgttc
      901 ggggcggtca cctacgcact cttcgccctc gactcgggta acgaagagta cctcgctgcg
      961 gcgaaggcgc agctcgccgc agcggaatga gcgaacgtcg atatagcccg ctcgcgacgc
     1021 tgttcgcggc gacctttctc ttccggatcg gcaacgcggt ggcggccctc gcgcttccat
     1081 ggttcgtcct gtctcataca aagagcgcgg cctgggcggg cgccacggcc gctagcagcg
     1141 tcatcgcgac catcatcggc gcgtgggttg gtggtggcct cgtcgatcgg ttcgggcgcg
     1201 cgcccgtcgc attgatctcg ggtgtggtgg gcggcgtggc catggcgagc atcccactgc
     1261 tcgatgccgt tggcgccctc tcgaacactg ggctgatcgc ttgcgtggtg ctcggtgccg
     1321 cgttcgacgc acccggtatg gccgcgcagg acagtgagct gcccaaactc ggccacgtcg
     1381 ccgggctctc cgttgagcgc gtctcgtcac tgaaagcggt gatcgggaac gtcgcgattc
     1441 taggtggccc ggcccttggg ggggccgcaa tcggcctgct tggcgctgcg ccaacgctcg
     1501 ggctgacggc gttctgctcc gtccttgcag gtctgctcgg cgcgtgggtg cttcccgcgc
     1561 gtgccgctcg gacgatgacc acgacggcga ctctctccat gcgcgccggc gtcgcttttc
     1621 tctggagcga acccctgctg cgccctctct ttggtatagt gatgatcttc gtgggcatcg
     1681 ttggcgccaa cggcagcgtc atcatgcctg cgctgtttgt agatgcagga cgccaagtag
     1741 cagagctcgg gctgttctcc tcaatgatgg gggctggtgg tctccttggc attgccattc
     1801 atgcgtcggt cggcgcccgg atatcagcgc agaactggct ggcggtggca ttttgtggct
     1861 ctgcggtggg ctcgcttctg ctttcacagt tgccaggcgt gccggtgctg atgttgttgg
     1921 gcgcgctcgt gggactgctg accggctcag tctctcccat tctcaacgct gccatctaca
     1981 accgcacgcc gccagaactt ctcggccggg tactcggcac ggtctcggcg gtgatgctgt
     2041 cagcctcgcc catggttatg cttgcggccg gcgcgtttgt cgaccttgct ggtccgctcc
     2101 ctggcctcgt tgtatcggcc gtgtttgcgg ggctcgtggc tctactctcg ctccgtcttc
     2161 aatttgctac caggaaccta cgtgcgcccg caccgacacc ctcacacctt cgaactactg
     2221 ttgccattaa ggggtcgttt cgtggtgctg aattttgacg atcggggtac cgtcacccat
     2281 cgggcgatat tgggggaaac ctgtacggtg ctggagatgg ccgcaggaac ctggcatgcc
     2341 gtgctgtcgc tggataccgg tggcataatt tttgaagtaa aacacggtgg ctatcaaccc
     2401 gtggctgccg atgactatgc gcactgggcg ccagcggaag gagaaccagg aaccacggag
     2461 cttatggcct ggtatgcgca agcgcaggtg ggcgacagcg cttttgccgt ctaaggcgat
     2521 aaacaaaaac ggaatgagtt tccccattcc gtttccgcta ttacaaaccg ttggtgacga
     2581 ttttagccgc cgacgctaat acatcgcgac ggctttctgc cttaggttga ggctgggtga
     2641 agtaagtgac cagaatcagc ggcgcacgat cttttggcca gatcaccgcg atatcgttgg
     2701 tggtgccata gtcaccgctg ccggttttat cccccacaac ccaggaagca ggcagtccag
     2761 cctgaatgct cgctgcaccg gtggtattgc ctttcatcca tgtcaccagc tgcgcccgtt
     2821 ggctgtcacc caatgcttta cccagcgtca gattacgcag agtttgcgcc attgcccgag
     2881 gtgaagtggt atcacgcgga tcgcccggaa tggcggtgtt taacgtcggc tcggtacggt
     2941 cgagacggaa cgtttcgtct cccagctgtc gggcgaacgc ggtgacgcta gccgggccgc
     3001 caacgtgaga aatcagctta ttcatcgcca cgttatcgct gtactgtagc gcggccgcgc
     3061 taagctcagc cagtgacatc gtcccatcga cgtgcttttc cgcaatcgga ttatagttaa
     3121 ccaagtcaga ttttttgatc tcaactcgct gatttaacag attcggttcg ctttcacttt
     3181 tcttcagcac cgcggccacg gccatcactt tactggtgct gcacatcgca aagcgctcat
     3241 cagcacgata aagtatttgc gaattatctg ctgtgttaat caatgccaca cccagtcttc
     3301 ctcccgactg ccgctctaat tcggcaagtt tttgctgtac gtccgccgtt tgcgcataca
     3361 gcggcacact tcctaacaac agcgtgacgg ttgccgtcgc catcagcgtg aactgacgca
     3421 gtgatttttt aaccatggga ttccttattc tggaagagac gaattaacaa caacatgaat
     3481 agtctgtcaa aacgcctttt tctatttata gtctaaccta aattccacgt gtgtttttta
     3541 ttagcttcaa aaatcactat ttcacgaaga atttagactg cttctcacac attgtaacat
     3601 tatttacaac
//
